LOCUS       DAEPXF010000001        86273 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain HB27
            SAMN13376389-rid12479423.denovo.001, whole genome shotgun sequence.
ACCESSION   DAEPXF010000001 DAEPXF010000000
VERSION     DAEPXF010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13376389
            Sequence Read Archive: SRR10518432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 86273)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 86273)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001069624.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001069624.1
            Genome Coverage       :: 315x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/15/2021 01:54:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,165
            CDSs (total)                      :: 4,076
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 89
            rRNAs                             :: 10, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 10 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 19 of 69
            Pseudo Genes (incomplete)         :: 44 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 10 of 69
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..86273
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13376389-rid12479423.denovo.001"
                     /strain="HB27"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="China: Hebei"
                     /collection_date="2017"
     gene            38..1156
                     /locus_tag="KRL67_000001"
     CDS             38..1156
                     /locus_tag="KRL67_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBH1520923.1"
                     /translation="MVVVKKAYKFRMYPNKKQQELINKTFGCCRFVYNKYLAKRIDVY
                     KNNKETFTYKQCSSDLTNLKKELNWLKEPDKFSLQNALKDLDNAYKKFFKEKAGFPKF
                     KSKKINRFSYKTNFTNGNIMYCGQHIKLPKLGMVKVRDKQVPKGRILNATISKEPSGR
                     YYVSLCCTDVDIEAFENTNNQIGLDLGIKEFCISSCGDFIENPKYLKKSLNKLAKLQR
                     ELSRKTIGSLNRNKARLKVARLQEHIANQRKDFLQKLSTKLIKENDIICIEDLQVKNM
                     IKNHKLSRLISDVSWSEFIRQLEYKANWYGRQIVKVGKFFASSQICNKCGYKNEEVKD
                     LNIREWICPSCNETHDRDINAGMNILKEGLRLITIQNK"
     gene            1275..1793
                     /locus_tag="KRL67_000002"
                     /pseudo
     CDS             1275..1793
                     /locus_tag="KRL67_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860778.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
     gene            1820..3457
                     /gene="rlmD"
                     /locus_tag="KRL67_000003"
     CDS             1820..3457
                     /gene="rlmD"
                     /locus_tag="KRL67_000003"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HBH1520924.1"
                     /translation="MSVKKKDVIEFEVDKMEFGGTSLSQIGDRVIHMKGGISGQKVRA
                     GVKKVRSKKAEVKMIELLEDSPLETEETCKHFRECGGCTLLSVPYEKQLEIKEKQVMD
                     LFLKQDLFGFQFLGIEGSPENKYYRNKMEYTFGDEVKNGPLTLGLHKKGKHIDIQTVE
                     ECMLVDEDFSKILVSSVEFFNEKKLPYYRTMNHKGYLRHLVVRKGIHTNEIMVNIVTS
                     SQEDFDMNEFKDMLLGIDLKAELVGVLHTINDGLADAVQCDELRVLYGRDYIQEEILG
                     LKFKISPFSFFQTNTKGAEVLYSIARDFIGDYNDKVVFDLYSGTGSIGQVMAGAAKKV
                     YGIEIVEEAVVAANENAKLNGLTNCEFIAGDVAKVVKDLKDKPDLIIVDPPRPGIHKD
                     AIRDICGFGAKEIVYISCNPKSLVVDLVDFKGYGYEIKMVKCMDMFPNTPHCETVALL
                     SKLDVDKHIDVEIKLDELDLTSAESKATYAQIKEYVLEKFGLKVSTLYIAQIKKKCGI
                     EMREHYNKSKKDNQAIPQCTPEKEEAIMDALRHFKMI"
     gene            3476..3757
                     /locus_tag="KRL67_000004"
     CDS             3476..3757
                     /locus_tag="KRL67_000004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001267199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH1520925.1"
                     /translation="MRWILKIILFPISLLLSILTAVLSFLLGIGTVILYLLMMFCIFG
                     AIASFLQKEVTIGIEALIIGFLLSPYGIPMVGASIIALLQGINEKIMSV"
     gene            3798..4316
                     /locus_tag="KRL67_000005"
     CDS             3798..4316
                     /locus_tag="KRL67_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013852041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClbS/DfsB family four-helix bundle protein"
                     /protein_id="HBH1520926.1"
                     /translation="MRVYENKEELKTEINKTFEKYIAEFDNIPESLKDKRIDEVDRTP
                     AENLAYQVGWTTLVLKWEEDERNGLQVKTPSDEFKWNQLGELYQWFTDTYAYLSLQEL
                     KSKLNENINSIYVMIDSLSDEELFKPHMRKWADDATKTAVWEVYKFIHVNTVAPFKTF
                     RNKIRKWKKIVL"
     gene            4417..5427
                     /locus_tag="KRL67_000006"
     CDS             4417..5427
                     /locus_tag="KRL67_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HBH1520927.1"
                     /translation="MYLFDEQPIVANKTLARELGLNEALVLQQINYWIEINKKSGKNY
                     HDGKYWTYNSIRAWQENDFDYMSFDTVKRTFSKLEKAGYLLVGNYNKDPRDKTKWYTI
                     NNDKLEELYMDMDLKKQKIQTEALKNSMPNALGQNAPMDKGKISQCNNADCTNAQVQN
                     ALMQKDNLHQPLPENTTNNTTNNSAYISIHPSIKEKNQLFSENMKGGRIDISYKNIYQ
                     NCLEELRHQTGYYEHMSIGNKSVAEEFDEILKILADVMVLDDESFITINQTKISVQVV
                     KERFRKLNSGHLEYLVGVLRENEYKIRNIRAFVLTAAYNAPSGIDAYYSALVSYDMRG
                     GY"
     gene            5429..5914
                     /locus_tag="KRL67_000007"
     CDS             5429..5914
                     /locus_tag="KRL67_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009346332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PcfB family protein"
                     /protein_id="HBH1520928.1"
                     /translation="MINEEIARKTLNMEINAGKVTGKGLLNLLKKLMKEAEKFGGLEK
                     LVNVNGNEVKLKDMVKKGQLEEIPVEEAELKELKKELNRYGVKFSVMKNKETGKNSVF
                     FQAKDMKVMDKAFKHALSKSEKKAERKESIHKNIEKFEEMAKNSISKEKIKNKQKEQS
                     L"
     gene            5953..8871
                     /locus_tag="KRL67_000008"
     CDS             5953..8871
                     /locus_tag="KRL67_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009257917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4368 domain-containing protein"
                     /protein_id="HBH1520929.1"
                     /translation="MQDKAKFLKQNIPYLAFFYVGNIFSHHVRAYTGGDVIDKIFQGI
                     LELNTMSFLPSFHPTDILIGVGVATLIKFIAYTKGKNAKKFRQGKEYGSARWGTRKDI
                     EPYIDEKFQNNILLTQTERLTMNGRPKNPKYARNKNVLVIGGSGSGKTRFYVKPNLMQ
                     MHSSYCVTDPKGTIVLECGKMLEDNGYEIKILNTINFKKSMKYNPFAYLRSEKDILKL
                     VQTIIANTKGEGEKAGEDFWVKAEKLYYTALIGYIFYEAPREEKNFATLLDMIDASEV
                     REDDETYMNPIDRLFEALEKKEPTHFAVKQYKKYKLAAGVVCSKRLLNQAVGKSLRTH
                     NLKPKKGAQVMRKNEKITALYERLSRDDFGKDDDQQRESNSISNQKAMLEEFAARQGF
                     TNIVHFTDDGISGTCFDRPGFLAMMKEVEAGNVEYLCIKDMSRMGRDYLKVGQIMEIL
                     RQRGVRLIAINDGVDSARGDDDFTPFRNIMNEYYARDTSRKIRSTFQSKGKSGKHLTG
                     TVIYGYLWNEARDQWLVDPEAAEVVKRIFAMTIEGYGPYQIASKLKEEKVLIPSAYLA
                     QHGEGVNKNKTFKDVYGWGSSTICNILEKREYLGHTINFKTRKHFKDKKSHYVPEDEW
                     TIFENTHEPIIDQQTFDLVQKIRGNVRRYPDGWGEAAPLTGLLYCADCGGKMYVHRTN
                     NGKRISQYTCSQYTKVPCGTLCKTQHRINEDVVLSLVSEMLKAIAEYAKHDRAEFVRV
                     VQEAQSSQQTAEVRKQRTRLATAKQRVSELEVLLCKIYEDNILGKLSDSRYATLDAQY
                     EKEQSELTAEISALEKAIKSYEKHEKDADRFIALIDKYENFDKLTIAMLNEFIEKILV
                     HERDRKGSIQTTQEVEIYFNFVGRFVPPAFGEVELTPEELEEIRKREERKDRLHQNYL
                     KRKASGAQKRCEDKIKKRKKAEIEAKKAAIRAEDIAKGVFVPVSSLPQREPMKGVQTA
                     "
     gene            8868..9212
                     /locus_tag="KRL67_000009"
     CDS             8868..9212
                     /locus_tag="KRL67_000009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019161271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TnpV protein"
                     /protein_id="HBH1520930.1"
                     /translation="MNITYTQNGDYLIPNIIIRKTKPLGHYGRLRKAYLEMHRPILFN
                     ELVLSDKLFEHCAEIDETARNRMELIVRSLAEQNGVTEQLKAENQMEWVRQMNACKAQ
                     AEDIVKFELIYD"
     gene            9359..9688
                     /locus_tag="KRL67_000010"
     CDS             9359..9688
                     /locus_tag="KRL67_000010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH1520931.1"
                     /translation="MNKDEVLKMSREENEGRHDEREIIAFGTASRAGMLVGALVCVLL
                     VFASELLFHIPEIGLVGWLVYFAMQGSSNIVLYKDLKNRRNLIWGIIEIAFAVAFAVV
                     LVIRSVV"
     gene            9691..9897
                     /locus_tag="KRL67_000011"
     CDS             9691..9897
                     /locus_tag="KRL67_000011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBH1520932.1"
                     /translation="MNEKLILKNNLKECRSEKGLSQQQLADMVGVSRNTISSIETGQF
                     NPTAKLALILCIALDKKFEELFFF"
     gene            9919..10173
                     /locus_tag="KRL67_000012"
     CDS             9919..10173
                     /locus_tag="KRL67_000012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006861338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HBH1520933.1"
                     /translation="MDKQTTGIVIAATKQWWLKVNRKPMRLHTLDGAEFPYIIKIEYT
                     VDGNAYTKRKWINAGDPVPKVGSNVQVMYNSDKPSKARIL"
     gene            10527..10886
                     /locus_tag="KRL67_000013"
     CDS             10527..10886
                     /locus_tag="KRL67_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015553292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3847 domain-containing protein"
                     /protein_id="HBH1520934.1"
                     /translation="MAKPKTLDQLRAEKERAETQLAQEQHKLERLENRKKYLEKGERT
                     KRTHRLCNLGGTIESLAPEVKDLTRTEMTELMEHIFSLSEVQRAVRHMAITHISQANR
                     ERELKADGTISSERHAD"
     gene            10852..12348
                     /locus_tag="KRL67_000014"
     CDS             10852..12348
                     /locus_tag="KRL67_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015559840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobA/MobL family protein"
                     /protein_id="HBH1520935.1"
                     /translation="MALFHLSVTQTKRSAGQSAIASAAYRSGEKLYSEYYGEYSDYTR
                     KGGVICSDILLPSHAPPEYADRQTLWNAVEKAERGKNAQLAYSFDIALQNEFSLEENI
                     ALARQFLLENFVSRGMVVDFAVHQPDREDGGIPNPHFHVLCPIRPIEQNGKWGCKQHR
                     VYELDEDGNRIRDADGNFVFNAVPTTDWGSPETLEYWRQTWAELCNAKFAEKELDVRI
                     DHRSYERQGVELLPTVHEGATVRAMEKKGIRTEKGEFNRWIKATNAVIRDIKKKIALL
                     FDWIAEAKAELAKPQAPDLVSLLNAYYTQRRAGAYSQKGKVSNLKEMNETFNYLRANG
                     IYSLEDLESRVSEHSAATESLKKTLDEQTARMKAIKQLYDSSAAFQSLKPVYDGLQKI
                     KFEKPRAKYKAEHADELKMFYAARRKLTGEFPDGKVDMKKLTEEYDELEQAHNTTYGE
                     FKTVRDDLHRLWKVKSCVDTAARFNERTEEQKLQNRPKTRHKKEDMTR"
     gene            12345..12647
                     /locus_tag="KRL67_000015"
     CDS             12345..12647
                     /locus_tag="KRL67_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006875985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH1520936.1"
                     /translation="MKKYDWKNEYNPIRRTAKRLLSGEALTDGRPLSDLIELDEHTRK
                     CLAFEATLTDRQIDMLQELGYLPVTTRQYGEECANCPYTLDEREHGVCRRCDVFKK"
     gene            12659..14530
                     /locus_tag="KRL67_000016"
     CDS             12659..14530
                     /locus_tag="KRL67_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015553294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBH1520937.1"
                     /translation="MQYTRAQIDRANAVSLEDFLRTQGETLIKSGREYRWKEHDSLTV
                     RGSKWFRHSQSKGGYPIDFVMEFYGKSFPEAVQLLTGESGEGQTEASTAPPTAFHLPL
                     HNRTADRAIQYLTESRGLNKTLVEAFLLSGDIYEDAKRHNVVFVGRDRSGTPRYAHVR
                     GTADPFRQDIAGSDKSYPFRYEGNGNQLFVFEAPIDLLSFICLYPQDWQKRNYLALGG
                     VSGKALDRFLSERKDTQKVFLCLDSDTAGSEACTRLAQSIPSEIAVIRLVPARKDWND
                     VLRQQGDIPSRKFIAETITLRELPTAQPVPMLRMADVELTSVDWLWFPYIPFGKLTII
                     QGNPGEGKTYFAMRLAAACTNRKPLPGMEKLEPFNIIYQTAEDGLGDTVKPRLMEADA
                     DLERVLVIDDRDTPLTLADERIARAIRENNARLVIIDPVQAFLGADVDMNRANEVRPI
                     FRSLGDIAQATGCAIVLIGHLNKAAGTQSTYRGLGSIDITAAVRSLLFIGKLKDSPTT
                     RVLIHEKSSLAPPGQSLAFSLGDEQGFEWIGAYDITADELLAGTDTAKTESKTAQAQM
                     LILELLADGKRMPSAELEKTVNERGISSRTMRTAKSRIGDRLVTEKDGTAWVCYLRD"
     gene            14673..14831
                     /locus_tag="KRL67_000017"
     CDS             14673..14831
                     /locus_tag="KRL67_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015524582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposon-encoded TnpW family protein"
                     /protein_id="HBH1520938.1"
                     /translation="MNNTATNTATCPTVRKQIGKTTYIVRVHFSQTAKETMEDKIKRL
                     LREEVRKM"
     gene            14873..15673
                     /locus_tag="KRL67_000018"
                     /pseudo
     CDS             14873..15673
                     /locus_tag="KRL67_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000565754.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TraM recognition domain-containing protein"
     gene            15837..16704
                     /locus_tag="KRL67_000019"
                     /pseudo
     CDS             15837..16704
                     /locus_tag="KRL67_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009731661.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
     gene            16913..17224
                     /locus_tag="KRL67_000020"
     CDS             16913..17224
                     /locus_tag="KRL67_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006270424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-strand DNA-binding protein"
                     /protein_id="HBH1520939.1"
                     /translation="MKQEMININANLLAEPTFSKFEKEGEKVEVANFTLVKKYGKGKE
                     YINCAAYGEKAKTVKAFEKGDLIHIFGYFKKREKDGKTYKNFVVKSYNKIEKKEENEE
                     E"
     gene            17228..17368
                     /locus_tag="KRL67_000021"
     CDS             17228..17368
                     /locus_tag="KRL67_000021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004830098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH1520940.1"
                     /translation="MEFFTHAVNVLKILVMAVGAGLGAWGVINLMEGYGNDNPGANAH
                     VP"
     gene            17486..17929
                     /locus_tag="KRL67_000022"
     CDS             17486..17929
                     /locus_tag="KRL67_000022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002571021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBH1520941.1"
                     /translation="MEIQGILKNLREKNNLTQEQLAERVQVTRQAVSRWETGETQPNT
                     DTLKLLSRVFDVSINTLLGSPRQLVCQCCGMPLNEDGLISREPDGSYNEDYCKWCYAN
                     GKFAYQSKESLLDYLVANMPNPDNADEETRRNQFDLYLSQLKHWK"
     gene            18093..18515
                     /locus_tag="KRL67_000023"
     CDS             18093..18515
                     /locus_tag="KRL67_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002571020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HBH1520942.1"
                     /translation="MTEDEAYKVHIQYTFNAFCKVVIRHAAIDIILKLRRRWEREVSL
                     DYLMNEKFVQLAEPEQLEEYLFTACGQTAVLYHAELAAALALLPEQTQEEIFRYYFLR
                     QPQRVIGVHIGRTRSTAGRHIQLALKRLRRLMEGKRYE"
     gene            18508..18705
                     /locus_tag="KRL67_000024"
     CDS             18508..18705
                     /locus_tag="KRL67_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002571019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBH1520943.1"
                     /translation="MSKLLPYETIVKAHEGDPDAIDTVLSHYVGYIRYCSKVHGKVSA
                     EVEEHIKQKLIAALFKFRFDR"
     gene            19164..20093
                     /locus_tag="KRL67_000025"
     CDS             19164..20093
                     /locus_tag="KRL67_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002571018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA adenine N(6)-methyltransferase family
                     protein"
                     /protein_id="HBH1520944.1"
                     /translation="MKNLGYSQNFLVNKKLVERLISKSNIDVTDYVIEIGPGKGIITD
                     VLSQHAGEVVAVEYDQELYNNLVRYHSHDNVTYIFGDFLKYKLPLNRRYKIFSNIPFQ
                     ITADIIRKLTDDVNPPSDINIIIQREAAKKNCGIPLQKYEGFRAAIIKAQYKVEITHS
                     FKRSDFFPSPNVDTVMLHMQLWDDRLSGDDLQNYKDLVAFFYTNIKGETAKERLSILF
                     SNEQIKRLGKANRISLSNSYTLITAEQWMNIYYYSKIGLTDEKKKKFQDAYKKLQKMN
                     KKLKKQNRTSLRKSSSNKKCRTRIDTSNGTKNR"
     gene            20426..21037
                     /locus_tag="KRL67_000026"
     CDS             20426..21037
                     /locus_tag="KRL67_000026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002571017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Type 1 glutamine amidotransferase-like
                     domain-containing protein"
                     /protein_id="HBH1520945.1"
                     /translation="MRTLFLTSSGLNEKTMVLFWKCIGKEPINTKAILVPSAAVGNDG
                     AREGIIVCMERLMNMGIPMNNILIYNLAFLLSDGYKRTYSSYISDIPVPFRLMSVQEL
                     TQYDMIAFCGGNAHTLLSEINRTGFSKPLKQAIENGLVYLGISAGSMIAAGNFSDGLG
                     YLANPLIPHAEKESPFGDISKNDLIELADGQTVLIKGNRQEII"
     gene            21265..21741
                     /locus_tag="KRL67_000027"
     CDS             21265..21741
                     /locus_tag="KRL67_000027"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH1520946.1"
                     /translation="MKGKILCPAGCQNPVARITKAKEILKNPRPAGSWRPLKCIFSGK
                     GKSRSQRAAKPRSGVILAKGKKAAHSGPQALSCPFYVRKNQTGAVCGSLRASFYHGAK
                     IRNSAVLSLAGNLLGSAFPKPCYGGLRRKKISVRQAVFLRELPRKYLTDQPIFCDK"
     gene            21759..22157
                     /gene="mobC"
                     /locus_tag="KRL67_000028"
     CDS             21759..22157
                     /gene="mobC"
                     /locus_tag="KRL67_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002586614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization relaxosome protein MobC"
                     /protein_id="HBH1520947.1"
                     /translation="MPKRYNTPHRSRVVKTRLSEDEYADFTARLAPYGISQSEFLRQA
                     IRRTAIRPIIHVSAVNDELLSAVGKLTAEYGRIGGNLNQIARYLNEYGVPYNALSGEV
                     RAAIADLAALKYEILQKVGDAVGNTQAYQL"
     gene            22129..23745
                     /locus_tag="KRL67_000029"
     CDS             22129..23745
                     /locus_tag="KRL67_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HBH1520948.1"
                     /translation="MATLKHINSKNADYGAAEQYLLFEHDEFTMKPVLDETGRLIPRE
                     DYRLSTLNCGGEDFAVACMRANLRYEKNQRREDVKSHHYIISFDPRDGPDNGLTVDRA
                     QELGEKFCAEHFPGHQALVCTHPDGHNHSGNIHVHIVINSLRIEEVPFLPYMDRPADT
                     KAGCKHRCTDAALRYFKSEVMEMCHREGLYQIDLLNGSKNRVTDREYWAQKKGQAALD
                     KQNAPMIAGGITPRQTKFETNKEKLRQTIRAALSAAASFEDFSSLLLREGVTVKESRG
                     RLSYLTPDRTKPISARKLGDDFDRAAVLAVLEQNAARAAEKAAAISSTPPSIQDQLRA
                     DRAARTAPNVQRLVDIEQKKAEGKGRGYERWATMHNLKQMAATLNVYQEYGFTSPEQL
                     EAAVDTAYQEMRQTSGELKALETKLQGKKELQRQVLAYAKTKPVRDGLKAQKSPKARE
                     AYRQAHESDFIIADAAARYFKAHGITRLPARKALQDEIEQLVSKKSGLYNTYHAQKQR
                     FKELQTVKRNIDQILRREEPRRRKEQSHER"
     gene            23735..24121
                     /locus_tag="KRL67_000030"
     CDS             23735..24121
                     /locus_tag="KRL67_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009259636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich VLP domain-containing protein"
                     /protein_id="HBH1520949.1"
                     /translation="MSDNLPRMDYRQHRRARRLVHECCNYDEGNCLLLDDGEPCVCVQ
                     SISFSLMCRWFRVAVLPLDGELAAALLYRGSRKRCAVCGAAFVPKSNRGKYCPGCAGR
                     MKKIKAAERKQKQRQKCHALEPFKPA"
     gene            24137..24988
                     /locus_tag="KRL67_000031"
     CDS             24137..24988
                     /locus_tag="KRL67_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002596232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HBH1520950.1"
                     /translation="MGVKGYAIHLSFSPGNSVLNAYKPSKSTRTQGGDPIADYIMADT
                     KLPAYLPYPRFLLKMEISQTAKLLYALLLDRSTLSQKNKWQDGEGRIYIIYPIAEIAE
                     ILDKGSTTIKGALNELDMAGLLERERGGFSAPNRLYVKVPRVPQVQFSDQLMAGKPTL
                     IEPENRPTDGQKTDLMMVGKPSPNQTTINNLTENQTKGVSGEPPAPYGRYQNIFLSQT
                     EYDELQAEYPDRLERFIEEMSRYLAASGKSYQNYAAALRIWAGNDKKGAPKKGIPDYS
                     CKEGESL"
     gene            24985..25827
                     /locus_tag="KRL67_000032"
     CDS             24985..25827
                     /locus_tag="KRL67_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015518395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBH1520951.1"
                     /translation="MKNEINAVLENMTTATPEPEDYTGEDGLLYCGRCRKPKEAYFAP
                     DKAAIFGRDRHPAECDCQRAAREEREAAEKRRRHFDTVEELKRRGFTDPTMRDWTFEN
                     DNGRNPQAGIARRYVEHWEDMRADNIGCLFWGGVGTGKSYLAGCIANALMEKEIPVHM
                     TNFALILNDLAASFENRNEYISRLCRYPLLIIDDFGMERGTEYGLEQVFNVIDSRYRS
                     GKPLIVTTNLTLDDLRNPEDTAHSRIYDRLLSMCVPVRFTGDNFRQEAAQRKMESMKK
                     LITD"
     gene            25842..26030
                     /locus_tag="KRL67_000033"
     CDS             25842..26030
                     /locus_tag="KRL67_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002571011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposon-encoded TnpW family protein"
                     /protein_id="HBH1520952.1"
                     /translation="MTDNKQHDTRTARRPDCVTEIRMGNSVLVVSGYFKKDTTTTAAD
                     KMARVLEAEAAATQKPAI"
     gene            26142..27761
                     /locus_tag="KRL67_000034"
     CDS             26142..27761
                     /locus_tag="KRL67_000034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009259632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HBH1520953.1"
                     /translation="MLRQATQNLITALYPRLSHEDELQGESNSISNQKRILEAFAKQN
                     GFTNLRWYTDDGFSGANFQRPGFQAMLADIEAGKVGTVIVKDMSRLGRNYLQVGFYTE
                     MLFPQKGVRFIAVNDNVDSANGGMDNDFTPLRNLFNEWLVRDTSKKIKAVKRAKGMSG
                     KPVTSKPVYGYLMDEDENYLVDEETAPVVQQIYQLCLAGNGPTKIARMLTEQQIPTPG
                     TLEYRRTGSTRRYHPGYECKWATNTVVHILENREYTGCLVNFKTEKPSYKVKHSVENP
                     VEKQAIFENHHEPIIDRETWERVQELRKQRKRPNRYDEVGLFSGMLFCADCGHVMYQQ
                     RYQNKNRKQDCYICGSYKKRTRDCTAHFIRTDLLTAGVLSNLRQVTEYAAKHESRFVK
                     LLIQQNEIGGKRKTAAATKQLEQAQERIAEVSRIIKRLYEDNVNGKISDERFMELSAD
                     YEQEQRELKDRAAALQAELDKSQAATVNAEKFMGIVRKHLAFEELTPTLLREMIEKIV
                     VHECSYDENGTRRQDIEIYYSFVGKIDLPEA"
     gene            28023..28247
                     /locus_tag="KRL67_000035"
     CDS             28023..28247
                     /locus_tag="KRL67_000035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004080809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBH1520954.1"
                     /translation="MNISYQPLWNTLKERGMRKEDLRLAAGLTTNMIANMGKGKHISM
                     ETLLRICNALNCDISDVIELEQDESTAEKG"
     gene            28279..28803
                     /locus_tag="KRL67_000036"
     CDS             28279..28803
                     /locus_tag="KRL67_000036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006060469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH1520955.1"
                     /translation="MKKIFLKIMLGVVLVCALFVGFLYANNDIGVTSTNLEADIRSSQ
                     KIKDDWTVDGSVSDTMAAYISYPQDMSDHTFSVYVNRPGLSFGYFFRGGGSLSGVERG
                     IVEFTVEEYKERAFISMNQQQVTQLQIDDGNSIQGISIDSNKPFAIVLPINAGTITFY
                     DVNGNTVEYWNNPL"
     gene            28904..29035
                     /locus_tag="KRL67_000037"
     CDS             28904..29035
                     /locus_tag="KRL67_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019214762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH1520956.1"
                     /translation="MSTNYSRAYLRHLFKINDPLAYRLATIHNLRFYTNLMEKLRKS"
     gene            29032..29844
                     /locus_tag="KRL67_000038"
     CDS             29032..29844
                     /locus_tag="KRL67_000038"
                     /inference="COORDINATES: protein motif:HMM:NF018758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH1520957.1"
                     /translation="MKEGTPMSIENIVQTVMTSKKYSNIIPETINRICNEESIKYKKD
                     KDILRSSKKRLHIISESFLSIANANKIASIKKCIDNDGHISTEMIEQLLLLHISTKER
                     SSFYKEFYNDIFRVVGNVNSILDIACGLNPVAYVLSDNFDSRVRYEATDINLDNIELV
                     NKILSLYDLQAKIYGGDILCDIPNGEWDVVFLFKIVPLLEQQKKGYFRSIIEQINSKF
                     FIITFPTKSISGKNVGMKDYYENMMTKYIENSQMEIIFRKAYFNEIMFVIKK"
     gene            29877..30251
                     /locus_tag="KRL67_000039"
     CDS             29877..30251
                     /locus_tag="KRL67_000039"
                     /inference="COORDINATES: protein motif:HMM:NF012799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH1520958.1"
                     /translation="MATPIGNLSDITLRAIETLKAVDIIASEDTRRTLILLNKYNIST
                     KLVAYHKFNEKEQATKLIELILQKDISIALVSDAGTPCISDPGSILISEALKNSIEVI
                     PVPGVSSVIAALSVSGFIFNKK"
     gene            complement(30285..31106)
                     /locus_tag="KRL67_000040"
     CDS             complement(30285..31106)
                     /locus_tag="KRL67_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009249954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBH1520959.1"
                     /translation="MKDHVSLQERLKDLRVEKGLKLEELAEKTGISKSALASYENEEN
                     RNKEINHGNLIALADFYEVSIDYLFCRTENREQINTPLSELHLTDEAVALLKSGRINT
                     RLLCEMMSHEKFAELMADAEIYVDGMATMRFHDINSALAAVRAMILEEHPEAAADRSL
                     KILEAGQIEEEDFFCHVTHKTWDAILHDIRKVHENDMESAPDTTPADELIKEVRKAMQ
                     STGDRVQEFLEIFCKAFQLKYKRLTDEERTTLKRLFKRSPLIKNSGINFRRRPWK"
     gene            31639..31824
                     /locus_tag="KRL67_000041"
     CDS             31639..31824
                     /locus_tag="KRL67_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002569227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH1520960.1"
                     /translation="MPRMSKKRKLELSFFLNDRGRVAYNDLCRKCQHECKQSFRAVVI
                     DCPHYLSKRAKRKEKTD"
     gene            31825..32166
                     /locus_tag="KRL67_000042"
     CDS             31825..32166
                     /locus_tag="KRL67_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008395954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR family transcriptional regulator"
                     /protein_id="HBH1520961.1"
                     /translation="MEFEFMTADTVLPPCMPLPTAMLRLPVSSTAKVMYARLLDATLT
                     GGAEDTNGILFVRFPIVELAAALSRSSVTVKRSLKELEDAGLILRVRRGVGEPNRIYT
                     LLPKKEGCRDE"
     gene            32159..32458
                     /locus_tag="KRL67_000043"
     CDS             32159..32458
                     /locus_tag="KRL67_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008395955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3847 domain-containing protein"
                     /protein_id="HBH1520962.1"
                     /translation="MNEKLEKLNQQIAQGEAKLRRAQHEEKILAHQIKQLTRKERTHR
                     LCTRGAMLESFLIRPEVLTDDDVMDILKQAFSQTGMKEVVAESVKCRVTGEPLTE"
     gene            32772..32975
                     /locus_tag="KRL67_000044"
     CDS             32772..32975
                     /locus_tag="KRL67_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005603655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBH1520963.1"
                     /translation="MKNRLEELRKQRGIKQEELASALEVSRQTIGSLENGRYNPSIQL
                     AFKIARYFHMTIEEIFIYEEDGQ"
     gene            32972..33403
                     /locus_tag="KRL67_000045"
     CDS             32972..33403
                     /locus_tag="KRL67_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005947481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH1520964.1"
                     /translation="MKSKITNHATIIIGLLLLAVSLYLIKTSNDPQGIMRTLPYVCIG
                     IGCGLFGHGMGNVISERAINSYPELKRQLDIEKNDERNVAIANRAKGKAFDMMTFVFG
                     ALMLSFALMGIDMVAVLLLVFAYLLVHGFALYYRFKFDKEM"
     gene            33535..35124
                     /locus_tag="KRL67_000046"
     CDS             33535..35124
                     /locus_tag="KRL67_000046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005426572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobA/MobL family protein"
                     /protein_id="HBH1520965.1"
                     /translation="MPCPHHDIKIVQRSNRQSAVAAAAYQSGERLFSEYDQKQKYYAE
                     KRGIVHTEIMLPPHAPPEYADRNTLWNAAEAVEKQWNSQLARRIVLAIPREIPPEQHA
                     DLIRDYCREFFVSKGMIADFAIHDKGDGNPHAHILLTMRAMDENGKWLPKSRKVYDLD
                     ENGERIRLPSGNWKSHKEDTVDWNDQKYGEIWRQGWAAVANRYLEANDRPERLDLRSY
                     ERQGLDKIPTVHMGPAVSQMEKRGIQTNIGNLNRDITAANRLMQSIRQMVRHLKGWIA
                     DLKEKKAALLEALQEAKEPTLPELLGKYLDLRREERTGWTSKGQLKGTVNDFNKVMDA
                     IRYLRDKELSTVESLDAYLDTVSGQVVSIRAEMKPKEKRMKEIDTLLSHIANFEAHKP
                     VHAEYAAIRFKKPKEQFAAAHRDELDAYNAAVRYFKVHLEGTKYSTKKLNEERTQLAG
                     EVAEYKERLSAVQEDVKILRDVRHWLNQVLPSEQYRQTAEPGKKPSIQQAVKGRAQRI
                     QEEQAEKRQQPRTEKKQDMEL"
     gene            35188..35823
                     /locus_tag="KRL67_000047"
     CDS             35188..35823
                     /locus_tag="KRL67_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008395964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HBH1520966.1"
                     /translation="MNVFEAVKQSVTTRQAAEHFGIRVGRNGMCVCPFHADKNPSMKV
                     DRRFHCFGCQADGDVIDFVSRLEAVSPKEAALMLAQEFSIPYEDKEPPSHRKPPQETP
                     EQRFRRMERYCFRVLCDYRNLLRRWKRDYAPNTPDEDFHPLFVESLQKQDYVDYLLDV
                     LLSPDMEERAAFIIDCGKEVSNLERRMAELTAGDTASRRTDHARSTAVPER"
     gene            35792..37129
                     /locus_tag="KRL67_000048"
     CDS             35792..37129
                     /locus_tag="KRL67_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008395965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence-associated protein E"
                     /protein_id="HBH1520967.1"
                     /translation="MQEAPPFRSVEEVKAMLESTEKGGVRNSIRNCLTVFQNDPLLSG
                     AIAKNLLTERTDIIKPIGYHRTGTAITDTDMNYLLLYLEETYGLTSEKKIAAAIGIVA
                     NENGYHPIRDYLNGLTWDGTERIRTCLHHFLGADSDQYTYEALRLFLLGAIHRAFHPG
                     CKFEVMLCLVGGQGAGKSTFFRLLAVKDEWFSDDLRKLDDDNVYRKLQGHWIIEMSEM
                     IATANAKSIEEIKSFLSRQKEVYKIPYETHPEDRLRQCVFGGTSNALDFLPLDRSGNR
                     RFLPVMVYPEQAEVHILDDEAASRAYMEQLWAEAMTIYRSGDFKLSFSPEMVCYLKEH
                     QRDFMPEDTKAGMIQAYLDRYTGSMVCSKQLFREALNHPFDEPKQWEIREVNDIMNHC
                     VTGWHYFSNPRMFPEYGRQKGWEREAPATDTSNGAEKIPEGFVEVTEQMELPF"
     gene            37371..37568
                     /locus_tag="KRL67_000049"
     CDS             37371..37568
                     /locus_tag="KRL67_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009249737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH1520968.1"
                     /translation="MAKTKTEIHVTTVFDGELDAADVFVSLIAQKYGQNTGKDNLAKT
                     QDLGYNKDEVQKNRVPSGLCG"
     gene            37575..39239
                     /locus_tag="KRL67_000050"
     CDS             37575..39239
                     /locus_tag="KRL67_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016288561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HBH1520969.1"
                     /translation="MMNGYEYTGMDAILAGSFRAAIYCRLSKDDDLDGESASIANQRD
                     MLESYCEKQGWEVVAVYQDDGYTGLNMERPDLKRMLKAIERRQINLVITKDLSRLGRN
                     YLQTGTLIEDFFPRHGVRYIAMNDGIDTMRDNNDIAPFKNILNEMYSKDISKKVHSSY
                     LLKAQQGQFTGCVAPFGYRKDPEDKNHLLVDEETAPIVRQIFLWALEGHGPNFIRRRL
                     EEQKVPCPTWWNRERGFRNVRTKWEKKDPENGRYMWDFSVIKDILMNPVYTGAIASQK
                     KDYRFKIGTIGEKKPQDWIVVEERHEPLIDSKSFAIVQDKLKSRQRPRQDGETSLFAG
                     LIKCGECGKSLTIRTTHAKHPQQIYACKTYGAFGKNHCSQHRVEYDTLYHLVLNKIRE
                     CAKAALTDGEAIAGKLTNTCEAEQKGQREALERSLTKDEERIEVLEKMVLRLYEDMVA
                     GRISEANFNLMLDKTQKEQAELKERVAQGRKKLADEMRLAMDAKQWVDAIQEYADITE
                     LDAATLNRLIKEIVVHEHIDSDKTRHISIEIHFNLKPIPEVEQVKG"
     gene            39329..39412
                     /locus_tag="KRL67_000051"
                     /pseudo
     CDS             39329..39412
                     /locus_tag="KRL67_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002838961.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
     gene            39432..40295
                     /locus_tag="KRL67_000052"
     CDS             39432..40295
                     /locus_tag="KRL67_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000466911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH1520970.1"
                     /translation="MFGIFDKIEEFFKEFLLGGIQANLESMFLDINDKVGAIATDVGK
                     TPMGWNGQVFSFIKSINDSVIIPIAGLIITAVLCIELINMVMQKNNMHDTDTFDFFKY
                     IIKMWIAVWLVSHAFEFSMAVFDVAQHMVNKAAGVINTSATVSGNQIVAMIDSLKDKG
                     LGELVMILFETSLIKVAIQVISIVIMLVVYGRMFEIYIYSSVSAIPFATMGNKEWGQI
                     GTNYIKGLFAIGLQGLFLMVCLGIYAVLVKTIKITDIHTSTFTILGYAVLLGLMMLKS
                     GTLAKSVLNAH"
     gene            40313..40573
                     /locus_tag="KRL67_000053"
     CDS             40313..40573
                     /locus_tag="KRL67_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HBH1520971.1"
                     /translation="MNKRKTVFIVVIGAGIMAVIDRIRLHSKVNDLEERKKDIGRCHN
                     DFCLMQERFNRSIDEQITSIQEEIGSVYEHIEELSKGKEDGR"
     gene            40577..40966
                     /locus_tag="KRL67_000054"
     CDS             40577..40966
                     /locus_tag="KRL67_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PrgI family protein"
                     /protein_id="HBH1520972.1"
                     /translation="MAYVPIPKDLKKLKTKVAFNLTKRQLIGFTIAGLIGIPIYLFMR
                     KVVPNDIAVIFLIVSTLPIFFITLFEKDGLTFEKYFKYIYLHKFYQPQKRVRKEVYLE
                     QKKKDSAAKVRTKQKEVKKSKTGLKAK"
     gene            40878..43307
                     /locus_tag="KRL67_000055"
     CDS             40878..43307
                     /locus_tag="KRL67_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000653482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBH1520973.1"
                     /translation="MNKKRKIQQLKLEQNKKKLRSQRQDLKQNKGKSKKDKGGLLDPI
                     FRKEPKRYTVEDTIPYLRLLKSGICQIDENHFNKSIAFEDINYQLALEEDRDLIFNQF
                     ANFLNSFDPSISIEFSYINQLGRNEEMKSAIQIPDKGDGFDDIRLEFREMLKSQIVKG
                     NNGLKKSKYVTFTVEADNLEQATSKLERMEIDILSSFKSMGVRAESLNGEERLKILHD
                     VLNPNKTFEFSYKDLKKKESTKTYIAPDEFNFTPSRYFKFGNFIGAASHFQILASELS
                     DRMLSEFLDIDDNINISFHIKAIEQSEAIKMVKRKNTDIDKMKIEENKKAVRSGYDMD
                     ILPSDLITYGEDVKSLLKDLQTRDERMFVVTIIFMNFARTIQKLDNTISQISSIANKH
                     NCKLKRLDHSQEQGLISVLPLGVNKIEIDRGLTSSSTAVFMPFTTEELFINSANSLYY
                     GLNALSHNLIMADRKKLKNPNGLILGTPGSGKSFSAKREMANAILTTDDDVIICDPEG
                     EYGNLVRQFKGKVIKVSSKSKDYLNPLDINMNYGDGDAPLKDKANFIMSMLELVVGGS
                     GLTAEEKSVIDRCLPKIYGKYFENPILENMPILQDLYDMLKNQEEKVGKKLATEMEIY
                     VTGSLNVFNNRSNVDLNKQLLCFDIKELGSQLKKIGMLVIQDQVWNKVSQNRGNKATR
                     YYIDEFHLLLKDEQTASYSVEIWKRFRKWGGIPTGITQNVKDLLMSNEIENIFDNTDF
                     VLMLNQASGDREILARKLKISQPQLKYVTNSNAGEGLLFFGNTIVPFLDKFPKDTILY
                     QKMTTKPEEVR"
     gene            43312..45474
                     /locus_tag="KRL67_000056"
     CDS             43312..45474
                     /locus_tag="KRL67_000056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HBH1520974.1"
                     /translation="MGKKLKKDFKERQRARIEKETLQSNMGIEPEESKLKYNDDYRGK
                     IVHDKDRFQDKVHEKTSKMTSDSEPVRGTSKRNARYRASNKDSITSSTETGGVSQEEI
                     GKADYGTEVKDGKIYDPLGKDLDNDGIIDRYDNDFRDSDYFESTYDVDGLKKDEISGN
                     PVNKQNTQKKNYKRKNYSDKLYTRKKDKEAKEEKADNKKSGKEAVLDREKKNRLSKEQ
                     ETIFENKTSKVSALSGLAKGSETVRDYLSHGSDENQGVEAGEKTADTSSKLIHGIKNY
                     SDKKRTKKGYDLTNKDYKIRKRKSKLEFRDAKEELKKTEEYQKANNFKRFQKRKQMKD
                     SIKRQNKSRLRDRIKEGLIGSLKSSKEVILRKTKGLMIIFIGLILLGTFFINFAGTGM
                     TGFMNSTSTVLTTSYLSKPNVLTEIHQQFSSMEQGLQDELESVEENYPGYDEYIVNKE
                     GDIGHNTHELLSYITSRFGEVKNVSEVSSVLDELFKSMYRLEYKEEIEIRYRTVTEIY
                     TDEDGNEYTESHEEPYEYKKLIVTLYKREMDSVVREVFQNYPDNVKHYEALLASLGNM
                     SDFFGSGSNDYSEIINNPNFGNPGIAFNEETVKRLFSEAERHIGKRYVFGANGPSNFD
                     CSSFVCWSFTHSGIKNMPRTTAWRIYTDYCNPVSTGEAKAGDIIFFKNTYNSGSPISH
                     VGIYAGNGMMIHAGDPIQYTSINSPYWREHFYGFGRPR"
     gene            45486..45719
                     /locus_tag="KRL67_000057"
     CDS             45486..45719
                     /locus_tag="KRL67_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HBH1520975.1"
                     /translation="MKKELTAVKNRIKKLKDKKALIDEELEPLFIREEELENEEIIAI
                     CRKNNITISDLMAKVNREKSEMKKENGNEKNEE"
     gene            45700..47274
                     /locus_tag="KRL67_000058"
     CDS             45700..47274
                     /locus_tag="KRL67_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4366 domain-containing protein"
                     /protein_id="HBH1520976.1"
                     /translation="MKKMKNKKAAGVILGAVLLIVGVVISLFAGSKIVFAQGGLFTTL
                     VNPIEAVAQIEVEVNVKYIFEDEKVFKEEKIKAEKGWLLDSGDLPIIPDDMKFIDEFL
                     FYEVKGDGKDEIIRKVTKIDVKDKETQTENESSKSEQSSQSNASKIENKGTQTELSKD
                     DISKMERESKELKEKLDKLNQEIKDKDKLSEKQKDKIKDLEEKIDKLKEKLEKGKDDK
                     DLSADMKKEIDKLTDKIKELEKKANEVGRAPIMHNPNVTPISPISGIKQGTGNVSPQT
                     SINSSGKGSATQESKGANTKDTNSNESKEKEKQVRYPNKLTPKQPANNGSHDSSLDGS
                     SKNINTNKGVASEPSKARGTVTENKDNANKNYPIHHNDSKDNKETDKYSADARQFVTF
                     TTKNGKTFHLIINHDEDSENVMLLTEVSEDDLLNMVEKKAPEKEIVKEESQKEEPKQV
                     KKEESSNTGTYLILILVVAGALGAGYYFKVIKKKEDKELEGFEEEDDDFFSEAEESEN
                     EVNEAVTEDKEDDEIE"
     gene            47370..49076
                     /locus_tag="KRL67_000059"
     CDS             47370..49076
                     /locus_tag="KRL67_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000779853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase 3"
                     /protein_id="HBH1520977.1"
                     /translation="MKLVIAEKPSVAISIAKVIGATKKKDGYYEGNGYKVSWCVGHLI
                     QMANPESYDEKYAKWNMADLPIIPREYKYEIAKSTKKQFTILKKLMNDKEIDIVINAC
                     DAGREGEAIFRLVYNQANCKKKMKRLWISSMEDSAIKEGFDNLKDGSFYDNLFESAQA
                     RAIADWLVGMNLSRLYSCLYKQNYSVGRVQTPTLAMIVKRDDEIANFKKEKYFTVELD
                     LDGFSLSTDRIDDKTVTEQLINLLGNSIEITDVIQKEKITKPELPFDLTTLQRECNKY
                     LNYSAKQTLDYTQSLYEKKLITYPRTDSRYLTEDMITSTINNIIGKHDFDTGRIKMVF
                     NSKRVTDHHAIIPTASSMNEDLASLPESELKVYELVLNKLHASVGYPLIENTTKIVAV
                     FDGFEFTSSGKVIVDEGFTKYLKKYIKKKNEDTVFPDLKIGDSLDIKEKEIKEKYTSP
                     AKHFTEDTLLKDMELAGNDALEKGVEVERKGLGTPATRAGIIENLIFKGFVERDKKNL
                     IATHKGISLVTIVSDTFKSAGTTAKWEMELSDIAQGKASKENFLKAIENEIKELVSTY
                     SK"
     gene            49160..49345
                     /locus_tag="KRL67_000060"
     CDS             49160..49345
                     /locus_tag="KRL67_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HBH1520978.1"
                     /translation="MSEVFMNNAKVMAKGQVTIPKKIREILEIENGDYVTFVVTEGKI
                     QIVNSKTFIEKNIQGRK"
     gene            49379..50425
                     /locus_tag="KRL67_000061"
     CDS             49379..50425
                     /locus_tag="KRL67_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1016 family protein"
                     /protein_id="HBH1520979.1"
                     /translation="MNKEKNSLQNINIEKNNNSFEEIVMIVENAKDRAYRKVNEELIL
                     MYQEVGKYISEKTKEASYGSGFVDNVAEFFSINYPDLKGFNRRGLYRMKQFYELYKDD
                     EKVSTLLTQLSWSNHLKIMSGAKSREEREFYINLAIKENLTHRELVRQMDSGYYERCM
                     LSNEGNMPAIQRAKQETHNLFMDSYVLEFLDAPKIGNERDFQKSILENLKNFVLEIGK
                     DFSFIGNEYRVQVGNHDYYIDLLFYHRGLSCLVAFELKIGEFKPEYVGKMNLYLEALD
                     REVKKQAENPSVGVILCASKDDEVVEFALSRSLSPTMVSEYRLKLIDKELLQRKLKEY
                     TGIAEQEIERHDEK"
     gene            50536..51342
                     /locus_tag="KRL67_000062"
     CDS             50536..51342
                     /locus_tag="KRL67_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HBH1520980.1"
                     /translation="MRTGNEFNGVEDTLYIPLAVRIYVSEKFPHFFYDEKALSLKPKI
                     PTGGIDNNSAEYFYMASVCRQQTIDMKIKKFLDENKKGNVVFLGAGLETSYNRIGNQI
                     VNFYQVDLPGVIEVRKRVLGNAKNEKLISGDMFDLNWIKEIDTSLPTLISVAGVYQYF
                     DEYKIIDMIQKLKAQFSKGELIFDATNSKGLKLANKYVKKTGNKNAQMYFSVDNSKDL
                     ADKTGTILLEVDGFFHGALKACQGLKLMTRLYMYFADKLHRTLVIHLRFN"
     gene            51455..60436
                     /locus_tag="KRL67_000063"
     CDS             51455..60436
                     /locus_tag="KRL67_000063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HBH1520981.1"
                     /translation="MRINDFHNILELVKQDILQSEAEYLKLLKVVGNNQRYDFRSQLS
                     IYDKNPEATACAKFDYWRERFNRTVMRGQKGIPILEDYGTYKKVDYIFDISQTVSKNR
                     DVNEVNLWRFDKEAHKDVLKEMIKTEGYEESESTLENIFSLSRLYGDEKIDNLMNELR
                     IADEDRISFTKFVRDSISYAVASRFKLDYPMDKELLKENFAMLDSISLMSLGETVSDI
                     SGTVIDTTIQKSKELELKKEVLRGKEAGYNKIKEELEEVEENVLRRDDQERISENERI
                     FRNGKYRRDNRENQGEYTESVRGRDGLYEGISESSVRSDEAELSFKQRRAEPLRDVSG
                     SIQGEEADQTPDGYSETGDRVYESRKAKTDDVLADREREPSSVQSNDFSPQRNDNQGS
                     SGNLKENTDKEIREADKASFSLPENSSGQIKLTIPLAQNDIDSILVNGGNHEGGRLPI
                     IAEFSKGKTVEKLGEYLKDTMGGGNGFFLNGTEVSSWYSDKGIHLAYGTSAREDMTQI
                     LSWSDAARRINELLENGEFATNVELLEAQDYERNRVSESLWYLYHDLSEEGKAQGYFD
                     FIETGGGFPKETRQLSEALKNPDYLKDVVSEYSRFLTGYKENKEVLRFHYHKVDSLYQ
                     RLQELALTRKEYTSNLIELPKIQGFITEDEVLATISRGSGVDKGKERITKFFKENHTL
                     QEKANFLKGEYGIGGGSHAVSGERGSGEWHDAKGLKLQKNHCNDVFLTWSNVAKHIDE
                     LLSKNLYLEEKNIEKKVEIEEQKEPSYYSKDDPYNLITDEMLEKVPELYAQEDVALAD
                     KQVHAAYIIPFRSNWTWYMTEYDRESKDAFGLVLGIEPEWGYFNLDELKELNAQRLVL
                     EDFPKTFRELKDSELKKQMDEQELYQVFNGELSFEENNIELEAPEDVEERKQAEPFQA
                     TLFDYLKEKEEVELNEKENSLSDEFTVKEGDTVYFNHEEYKVRDLSKNQITGKNDLWL
                     DPVRQGNHQIPIVAFTDNEDLLRQISLERPEFIIGDEVKYKGKDYAITKFDNMGNNLK
                     TVTVKDNTEYLGGMITGSDVIPYRQESELNKIFENLTYKKEELYDLAVRIDEIAYDFD
                     SYDYADSVEAESEKRLDNINQIYGDLQAGNVSVYINSLNEMIEEDVEDTEIIKQVLTQ
                     LEEKKIDIQPKQEVFNFKITEETLPQRLSPSERLNNNLEAISMLNRVESGERELDITA
                     QEVLAKYVGWGGLSDVFDESKDGQWKDARAFLKENLSSSEYEAARESTLTSFYTPKIV
                     IDGIYKTLSGMGFKQGNILEPSMGIGNFIGNVPDEMGKSKFYGVELDSVSGRIGKLLY
                     PESEVQVKGLEETGFSNNFFDVAIGNVPFGEYKVNDREYNRNNFLIHDYFFAKSIDKV
                     RNGGVITFITSSRTMDKKDESVRRYLAARAEFLGAIRLPNDTFKGVAGTEVTSDIIFL
                     KKRDSVLERDEAWIHLSEDKNGLVYNKYFVDHPEQVLGSMREVSGRFGKTLTCEPIAY
                     LGQEINMASLKERIEIAGERISKDTKYEEIELLDDEITSIPATDDVKNFSYTLNDDEV
                     YYRENSLFIKKEVSDKNKEKIKDYLELNVALKDVIYKQKEDFSEEEIKASQEKLNEVY
                     DNFSKKHGFVNNLSNTRALKEDSNFPLVSSIEILDEEENFKAKGDIFSKRTITKAKVI
                     DHVDTSLEALVLSVSEKGYVDFDYMGSLTGKDRATLIEELRGEIYLNIREEQNFYRPL
                     SFNPEDGDLPFACANGSNSYKYGYVTKDEYLSGNIRDKIAIVDSYLSKLRQTERELPH
                     LGFVENGKEKELISYEMNRLEYQKAELTKVLPKELEASEINVRLGATWIPIKDIEKFI
                     FETLKTPGYARWDIKVKFSNITSEWNVEGKSRDKGNDLAEMTFGTSRVNAYKLIEDAL
                     NLKETKVFDQIINPDGSKTSVLNKKETMLAGQKQELLKEEFKNWIFNDQERRNRLVKL
                     YNERFNSIRNREYDGSNLSFEGMNTEIDLRPHQRNAIARSLYGGNTLLAHVVGSGKTF
                     EMVASAMESKRLGMCSKSLFVVPNHLTGQIGREFMQLYPSANIMVADKKDFEPKNRKR
                     FIGKIATGEYDAVVIGHTQFEKIPMSKEYQEKHIQDQINEIVNYVEEYKHDRNQNFTV
                     KQLEKTKKKLETRLEKLNDDFKKDDIITFEELGVDMLFVDEAHNYKNLYLYTKMRNVA
                     GIGQSEAFKSSDMFMKCRYMDEMTGGKGIVFATGTPVSNSMTELYTMQRYLQYESLKK
                     NHLEHFDSWASTFGETQSSFELSPEGTGYRVKTRFSKFYNLPELMSMFKEVADIQTAD
                     MLNLPTPEANYEVIKTEPSEEQKEILKSLSERADDVRNRVVEPDVDNMLKITNDGKKL
                     ALDQRLINPLLPDNPDSKVNVCVKNVFAIWDKTKENRSTQLLFSDMSTPKGDGEFNIY
                     DDIREKLVAMGIPKEEIAFIHEANSDKQKDELFAKVRKGEVRILMGSTQKMGAGTNVQ
                     NKLIALHDLDVPWRPADLEQRAGRIVRQGNENEKVNIYRYVTENTFDAYLWQTIENKQ
                     KFISQIMTSKTPVRVAEDVDESSLNYAEIKALATGDPKIKEKMDLDNEVTKLKMLEAN
                     YKSNRYRLEDKVAKNYPEEIARTEKLIEAVKKDIASVEPKAEGEEKFTSITIAGEKIN
                     DKKLAGERLLEAISKVKINESKVIGKYRNMDLEVSYNFFTNEHNFSLNGAAKHSGELG
                     TSADGNITRLDNALEKMPEKLNRLEEKLISTKEQLQNAKEELKKPFEKADELKTKVLR
                     LAELNKLLDMGEVEEKRNDNPLVEDVKMAIIDFCNREYEENHSYDEFNTLYPDLKHIG
                     IAYTNTPDERHGIQYELDLEEKTWTQYIDGTPIKTESFYYENKGENEALRNMKNEIEL
                     FSFNDLVYVDSEDLKAALGLDIDDEGNFYDPLSKDLDNDGIADRYDNDFRDSDYFEST
                     YDVEDNLNTKVESVTKAEDKPSILSQIRAYQNESKTEEKQKTKEQEILR"
     gene            60479..61138
                     /locus_tag="KRL67_000064"
     CDS             60479..61138
                     /locus_tag="KRL67_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006627214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HBH1520982.1"
                     /translation="MDYKTMINQIEDMVNYNHKDFVKAIISMEKGINDESALDKLYDA
                     YMDNDSLNLLHEEFDYMIEDLREQGQIKDLPYVQEEKDNLINIVGNIVGEVDVVEREN
                     KNGEAFKVANFSVVSKDDEGNKVYHNCSAYGGKSDIPKDFKQGDFVKLFGQIRTSIDD
                     NGKEHRNIRILSSKLLKEKEQMKGQEEKKESVLGAIKKYQAEDKEKPKEKKEANQEAE
                     R"
     gene            61279..61491
                     /locus_tag="KRL67_000065"
     CDS             61279..61491
                     /locus_tag="KRL67_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001263549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBH1520983.1"
                     /translation="MRAFSYKRLFKKLIDLDMTNNELMEKAKVSKSTFYKMKNGQNVT
                     TDVLLRICNALDCDIEEIVECIRIDK"
     gene            61512..62540
                     /locus_tag="KRL67_000066"
     CDS             61512..62540
                     /locus_tag="KRL67_000066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009643545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4297 domain-containing protein"
                     /protein_id="HBH1520984.1"
                     /translation="MSKEKYYMNLPKDLSGSIAKNRFRLELLWGISKIIDEHKANNEY
                     TIIFDFKCDIELHKEDELDFYQIKTKKSGNYNSNNLCKKGKNKNNSILGKLYALYSPN
                     YNIKLAIVCNKQLKINNKEIDFPEQCFGDLDQDVLDDVRKKLCTELNLDTVCLDNVFY
                     IFDNMDLLNPEDSIRGKLVKSFVDIKGEEPQNPNALYRLVVDSVKEKASYEFDSGTYE
                     DVVKNKGITRSEFDKMLNAHKKESKNGINETQEYINNLSFAKRRRYNTALGNILETQQ
                     SESLRLIKIKIYNYIVEHENSLDDIESYLKEISKLFDDDFDVEFTDDMKSVQYIMIYY
                     MYASGGIL"
     gene            62540..64222
                     /locus_tag="KRL67_000067"
     CDS             62540..64222
                     /locus_tag="KRL67_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytoplasmic protein"
                     /protein_id="HBH1520985.1"
                     /translation="MNVIFDKIYIFDVVKKKAYSTEFKKGVNIITSSDIDGTDRGKSV
                     LLRSLYHALGADSHFDSKWGEKDKVYILFFKVDDKDFSIYRSQKLFKIFNAKGDLIFT
                     TIHRSELADFFGNLFNFSICLPQKNTNQLVIAPPAYSFLLNFLDQDEYIGTKFNSFKN
                     LAQFSNFKLNVIYSHLGIYNKEYFELVKHKEELELEIKSKKEEIEDLIKMKDRTILIL
                     DGFSCPETSSALENELNIETQKYSSLINEMNLVRNKLVDLRNQLVEQNITLSQISKFE
                     IKEEKEIKTILKSKVCPECHSVLDSTLELRSKRYNHIDNALSLKDAIKTENAHIEDEI
                     LKNEGIYSNLTLVLEEHNKKICKNKKEIKDYIKFRGLNKLIDEINNDLTINSRVIDST
                     KEALKPLKKEFKSVKDKIKSVDENYYYLIDKLKIRFNLNELEADSYEQLSKNFCASGS
                     NKPLSTVIWYLTLNDLKQKFNPEGTTFPMVFDSPNNAETDQEKKQALVEYIMESSDQF
                     SQLIVSAIGFSEKDYSIDSNINIKVLENEKYSLLNSEMYVQNYELLQCMNDA"
     gene            complement(64249..65580)
                     /locus_tag="KRL67_000068"
     CDS             complement(64249..65580)
                     /locus_tag="KRL67_000068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HBH1520986.1"
                     /translation="MAITKIHPIKSTLNLAISYIVNGEKTDEQILVSTHKCHQETAHT
                     QFLRTRNDVGTNGTVLARHLIQSFLPGEASPEMAHQIGMELCKKILKDEYEFVLSTHI
                     DKGHIHNHIIFNNVNMVTGKCYHSNKKSYHQIRYQSDKLCKENNLSVIDKFYESYKRK
                     YKTNGKSWYENEQSKKGTSWKSKLQFDIDRLIKQSKDWEEFLKKMAELGYEIKYGKHI
                     AFKPKDKQRFTRAKTIGEDYTEERLKERITENQTIKTPPVKKRIGNVIDMNTNVKVKE
                     SKGYEYWATKHNLNTMAESVIYIREHGIKSVKQLDEYIQKVADERQNLQDKIKIIDKE
                     MQELSTTMEKVHTVKKYKQYYMEYKANPSDKAFFEEHKAEITRYETALAKLKKSYSKL
                     PDSKGILDKLDKLQEKKNTLMQEYSSTKSTMDELYQIRKNYGIYMDKEMER"
     gene            complement(65586..65936)
                     /gene="mobC"
                     /locus_tag="KRL67_000069"
     CDS             complement(65586..65936)
                     /gene="mobC"
                     /locus_tag="KRL67_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006270454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization relaxosome protein MobC"
                     /protein_id="HBH1520987.1"
                     /translation="MANRIRNERLEIKLTEEEKALFEEKRKLARCRNMSHFIRKCVLE
                     KEIYQVDLEPFRDLQGLLSNATNNINQIAKRVNSTGVIYKDDINDIKNEIEHFSKELW
                     QIHSLLLNRTSGGD"
     gene            66245..67402
                     /locus_tag="KRL67_000070"
     CDS             66245..67402
                     /locus_tag="KRL67_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA family protein disulfide reductase"
                     /protein_id="HBH1520988.1"
                     /translation="MKKIVSRGACLLLALCLSLNAVGCQKSNENNKIKEGQSISSEEA
                     GMSINANGKNETFKPSDYTLEAKKEYVYEYLGLKFKLSDKFRNYIADKKIAMLDDQSP
                     IDKELKYAILTFEKMTEEQKNAVIEKMGDGYKNWQNELERIGTIGIFEKNTSEEKISK
                     ITKCDTHTKIGVSSDGKYDCYLSTNSGAESNLLDELKRTEIQIIDKKERPKNGFVLSE
                     KTDLENTEAFNKESVKDLRKLSTKDINGKDFTSKDFEKYDLTMVNVFATWCTACVKEI
                     PDLVEVQNEMKSKGVNIVGVVTDTVDDNGENKEAIEKSKLIHEKTKASYPFLMPDKTN
                     FNGRLNGIQAMPETFFVDSNGNIVGDTYSGAKSAKEWKQVIEKELEKIKNK"
     gene            67580..68470
                     /locus_tag="KRL67_000071"
     CDS             67580..68470
                     /locus_tag="KRL67_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HBH1520989.1"
                     /translation="MDRIKNIINGSTKKDKYKIRDRFRHLLQSVWFLITNSYFEGFKT
                     GKIYGGNLKKICVPGMNCYSCPGAKGSCPIGSLQAVIGNSKYKISYYIVGILFFIGAL
                     VGRFVCGWLCPFGLIQDLLHKIPFFKKVETFKFDKHLRKLKYIILLVFVIILPLFLVD
                     ILGQGSPYFCKLICPIGMLEGGLPLVLLNKSMRSAIGFLYYWKGIILIVTLLLSIIMY
                     RPFCKYICPLGAIYSIFNPISIFRYRLDKDKCINCGKCKKVCQMNIDPVENCNHLECI
                     RCGRCKNACPVDAISYGVRK"
     gene            68489..69766
                     /locus_tag="KRL67_000072"
     CDS             68489..69766
                     /locus_tag="KRL67_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBH1520990.1"
                     /translation="MIKNAIAYITRKRNRTLIIFIILTIVLSCLYSCLTIMKSSNEIE
                     KALYESSNSSISITKKDGKYFNVNQFKDIEKLKEIEEKIIQYDGLAKLKDAKVVSGEQ
                     RINREDLSDEFKNVVSLEATNNTKRNILFSSGVFTIKEGKNIGENDKNSIIVHEEFAK
                     QNNLKLGDEVDLELLDIEKSGKIKSHKFKIIGIFSGKKQEIYTGLSSDFSENIVFVDY
                     STSQEILNKSENNKIANKILMYSGSAESTDLALNKLKELKIDESKYFVEKDSNAFEES
                     LESVSGIKHIIKIMTYSIMLGGMVVLSLILILWLRERIYEIGIFLSIGTSKIQIIMQF
                     IFELIFISIPSIISSLFLGNVLLKVIVDGFINSEDSMISGGSLINNSSFMLNITTLGQ
                     SYLILISIIVLSVVFASSLILIKKPKEILSKIS"
     gene            69776..70432
                     /locus_tag="KRL67_000073"
     CDS             69776..70432
                     /locus_tag="KRL67_000073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004612033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBH1520991.1"
                     /translation="MDILEIKNVAYSYANSKEKVLSGVNQKFELGKFYAIVGKSGTGK
                     STLLSLLAGLDKPQTGKILFKNEDIQKKGYSNHRKNNISLVFQNYNLIDYLSPIENIR
                     LVNKSADESILFELGLDKKQIKRNVMKLSGGQQQRVAIARALVSDAPIILADEPTGNL
                     DSVTAGEIINILKTLAKDRNKCVIVVTHSKEVADSADIILELSGKKLKKVNKMNLEVE
                     "
     gene            70432..71808
                     /locus_tag="KRL67_000074"
     CDS             70432..71808
                     /locus_tag="KRL67_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBH1520992.1"
                     /translation="MIKNAFAYVTRKSLKSLIIILVILSMSTLSLISLSIKDATDRAS
                     KETFDNITNSFSMEINRQVNPGTPRGGGNVKGEDIKKISQTDSIDSYVKRINSVADLV
                     DYDIIETQETLANQSPERAKNFKRTVMLTGVNDSSKETKFVSEAYKLVEGKHLENEDK
                     NKILMHKDLAEKNNLKVGDKIKIKSNLFDADNEKGADETVEVEIKGLFDGHNSGGVSA
                     AQELYENTLITDVHTAAKVYGNTEDTAVYQDATFFVKGDKNLDSVIKDLGKLDINWRE
                     YNLIKSSSNYPALQQSISGIYSISNKLFVGSLIFAGVVVSLLLFLWMNARKKEIAVLL
                     SLGISKLEIFGQFLIEMVFISIPAFVGSYFLAQYTADKLGNNILNKVTGDIAKQIARQ
                     SASSQLGGGAEAEGFNKTLSSLDINVLPKFIIYVVIFMSLVLLVSLIISSFNILRRNP
                     KELLIDNN"
     gene            71906..72559
                     /locus_tag="KRL67_000075"
     CDS             71906..72559
                     /locus_tag="KRL67_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBH1520993.1"
                     /translation="MKILTVEDDNMIREGISEYLSEFGYTVIQAKDGREALSKFNSDI
                     NLVILDIQIPFINGLEVLKEIRKKSNLPILILTAFSDEEYKIDAFTNLVDGYVEKPFS
                     LPVLKARIDSLIKKNYGHLEKFEYKDLSVNFNSYTAKINGEEIDVNAKELEVLKCLLD
                     NNGQVLTRMQIIDYVWKDSEETPYDRVIDVYIKELRKKLQLDCITTIRNVGYKLERK"
     gene            72556..73875
                     /locus_tag="KRL67_000076"
     CDS             72556..73875
                     /locus_tag="KRL67_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBH1520994.1"
                     /translation="MKKLKIFPKMFIQIFSVLGIIIILVHSLVFFIFPKTYLETRKEK
                     IHNMANEISSNMNGKEIKYIEQTLELYSKSSEIKAFIKEKNNKNEIQIKDNINVNLES
                     DSNSLIIEEREIKLNDGKKTNLQFVSTADMQKDAKDLSLKFLPYSLLISILFSAIISL
                     IYAKSIKNNIQEIKIVTDKMMKLDKKTRLKVSSNDEVGELKQQINDLYSTLLRTIDDL
                     EFKNKEILKLEKLKYDFFKGASHELKTPLASLKIILENMKYNIGKYKERDIYINECIE
                     IVDSLTKNISQILSVHSIENLNNDEEYLKINDTLEDILKKYEILANQKKITVNNYILD
                     ENVYIGKTALKIILSNLISNAVKYTDVNGVINIGIVNDWLYIENSYGNNRISNMDKIF
                     DVKFDLNKENSNGLGLYIVSNILNNYNIEYKALQDEEFFIFKIKIFS"
     gene            74399..74809
                     /locus_tag="KRL67_000077"
     CDS             74399..74809
                     /locus_tag="KRL67_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004634151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HBH1520995.1"
                     /translation="MAKEYYLYIRGQKVKVSEDIYIVYWREKEHEKYLEQVDRKNHLL
                     FFSALDHDGHFTDNIVDESVDVEKIVETQMMIEAVRSAITRLNAEERDIIERLYFNDE
                     TVRSVAKLKSITHPALIKRRNKILEKLKKFIEEL"
     gene            75298..75450
                     /locus_tag="KRL67_000078"
     CDS             75298..75450
                     /locus_tag="KRL67_000078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003062685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH1520996.1"
                     /translation="MDNDWNGLADLIANLITKYAGVLDLENLPNPTPVKNTEDKNKFD
                     IAKSTD"
     gene            75551..77233
                     /locus_tag="KRL67_000079"
     CDS             75551..77233
                     /locus_tag="KRL67_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HBH1520997.1"
                     /translation="MSKEKIKVYLYTRVSTSIQIDGYSLEAQKSRMKAFALYNDYEIV
                     GEYEDAGKSGKSIEGRVQFTRMMEDIKSGKDGVSFVLVFKLSRFARNAADVLSTLQIM
                     QDFGVNLICVEDGIDSSKDAGKLMISVLSAVAEIERENIRVQTMEGRIQKAKEGKWNG
                     GFAPYGYQLIDGKLIINEEEAIAIRTIFDQYVNTSIGANGLSKYLENHGIRKIPRQNG
                     KNPLFDAGLIRKILKNPVYNGKIAFGRRTLEKVHGTRNEYRQVEQDDYLVAEGIHQAI
                     IPDELWQAAQVKLKAQAKKYEHVNKGKNMRTHLLSGIVKCPICGAGMFGNKSIKYKKD
                     GTKYKDFYYYGCKHRLMNRGHKCTYNKQIREELLDDAVAEVIIKLVSNPKFASMIQEK
                     INMKVDTSAIENEIDNYQNELRKSHSTKYKLIEEIDNLDVEDKHYKRRKNDLDDRLYR
                     MYDKIEELESQLIEAKAKKETIEVEKLTGDNIYKVLIYFDKLYKVMNDVERRQLIEAL
                     ISEIQIYEEKQPNGQWLKSITFKLPIIDEDLNISLENDEQVETCVLLSHKNS"
     gene            complement(77212..78668)
                     /locus_tag="KRL67_000080"
                     /pseudo
     CDS             complement(77212..78668)
                     /locus_tag="KRL67_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002576182.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
     gene            complement(78717..78878)
                     /locus_tag="KRL67_000081"
     CDS             complement(78717..78878)
                     /locus_tag="KRL67_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH1520998.1"
                     /translation="MENIKNIQGDAIKAPAPNHTFEKTKAYQDFINVLSQIIEKHGAE
                     VLGEINRAN"
     gene            complement(79455..79760)
                     /locus_tag="KRL67_000082"
                     /pseudo
     CDS             complement(79455..79760)
                     /locus_tag="KRL67_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326549.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
     gene            complement(79880..80377)
                     /locus_tag="KRL67_000083"
     CDS             complement(79880..80377)
                     /locus_tag="KRL67_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBH1520999.1"
                     /translation="MKLENWSIEYINDLVKNANNRNVSKNLRNIFPYPYSVVDAEKFI
                     THCQRCNNKHINLAIIEDDKAIGSVGVSIGEDIYEKSAEIGYWIGESYWGQGIMTHAI
                     KEMIELCFSQYDIVRLYAVVFSHNKASCKVLEKCGFVFEGTLKNSIYKDGNIYDSRIY
                     ALVKK"
     gene            complement(80379..81305)
                     /locus_tag="KRL67_000084"
     CDS             complement(80379..81305)
                     /locus_tag="KRL67_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH1521000.1"
                     /translation="MNVAITRILNILGDVKIVDKIVNLNSSDINSFLLSVFQSAVIKK
                     TPQNILAAFSTNRFTSPSHFDPIAFHNLEVKLLSLSEKLGIKNLLLSPSAPLGSCSVF
                     GCVNQNNVISALRGTETLSDPSNMIAIIIADGLKKKILRNEDAIHHATTTRVVRAQAF
                     EGKGFYAHFGIFCIVSSGKDKGSYSCESELLIKHLNYYKDLFTNIENSKISICLRKRG
                     GYPDRDGFFEKMTAIVKSSLPEIQITIDLNSEDNAYYKGINFKIYLHCNDDVIEIADG
                     GFVNWISEMTGNKKNRCLISGIGIDRLLTLEE"
     gene            complement(81421..82044)
                     /locus_tag="KRL67_000085"
     CDS             complement(81421..82044)
                     /locus_tag="KRL67_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HBH1521001.1"
                     /translation="MNFIDIMPKEVKHKLQEKSYAAGNTILYAEEENNFVYFLLSGVA
                     EAYIQSSNGNFSTLYLYKSGSFFGEIEQFYDGKKPVEITALSDCIVKRLYRTYFLEWI
                     KNDFNATTFLIQGLAEKLIINSQLVENILELTVKERLLRSISLHYHRNTLDSLNKSRL
                     AKEVNSPIRSVNRAIDECKKQGLLDFSDNKFIIINPKELLKFLPYIE"
     gene            complement(82047..82235)
                     /locus_tag="KRL67_000086"
     CDS             complement(82047..82235)
                     /locus_tag="KRL67_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBH1521002.1"
                     /translation="MDYISVKAASEKWGISERRIQKLCEENRINGTEKFGRAWMIPKD
                     AQKPIDGRYKSTMKVGEI"
     gene            complement(82493..82588)
                     /locus_tag="KRL67_000087"
                     /pseudo
     CDS             complement(82493..82588)
                     /locus_tag="KRL67_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003253182.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
     gene            complement(82667..83030)
                     /locus_tag="KRL67_000088"
                     /pseudo
     CDS             complement(82667..83030)
                     /locus_tag="KRL67_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002578545.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
     gene            complement(83122..83268)
                     /locus_tag="KRL67_000089"
     CDS             complement(83122..83268)
                     /locus_tag="KRL67_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017353027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HBH1521003.1"
                     /translation="MLLENGTNIKDIQHILGHAKISTTMDTYSHVTNNMRNQTVNVFE
                     SIVE"
     gene            83563..84597
                     /locus_tag="KRL67_000090"
     CDS             83563..84597
                     /locus_tag="KRL67_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YHYH domain-containing protein"
                     /protein_id="HBH1521004.1"
                     /translation="MFKKKLIAVAMSITMISVGSFSYAHSGRTDSSGGHRDNKNKSGL
                     GSYHYHCGGYPAHLHNNGGCPYTGGGSSSGTTTSVNNEEKQKRSVGEKGYNQGYEDGY
                     KGNYSSSNYSGDYSDTYESKYSEGYEKGKAKLEEEEKVAKETGYNLGITGAKSNNTYE
                     KEALKNAYDTGYSTGYNEYKTKKIEEYKAKGIEDSNKDKEKMTFEENIDSEFIDAYNN
                     AYDEIQEQLKNDYTTQGFESAIKGERFDTSTIGNVKYANWFKEGYDNGKVKLPKVKES
                     VYNQGYNEEDFSVPDEMKSIETKLKGVYDEGLEKREEEKSRNATYGVGAGTAAVVAGV
                     VYKKKKRKKI"
     gene            84684..84887
                     /locus_tag="KRL67_000091"
     CDS             84684..84887
                     /locus_tag="KRL67_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4236 domain-containing protein"
                     /protein_id="HBH1521005.1"
                     /translation="MGFNFRKSINLGGGLKLNLGKKSVGISAGVKGARVSVNSKGRKS
                     ATLSIPGTGISYTKTSTKKRKPK"
     gene            85058..86218
                     /locus_tag="KRL67_000092"
     CDS             85058..86218
                     /locus_tag="KRL67_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp27"
                     /protein_id="HBH1521006.1"
                     /translation="MKLIKTLLAIGMSVSLLIASCPTSNALSSISSIKGTNKYETAGL
                     IADKQNYDIAILINSENGLSDGLSASGLSGAKNAPILLTKKDTIPSETLKRLEKVKEV
                     YLIGGKNSIGASVQKILSDKKISINRISGTDRIDTSHKVAKEIGRVSTVKKVILTNAF
                     KGEPDAMSVASIAVRDKSPIVLTDGNTTSFATDGLETYAIGGKVSMSDKLINETKATR
                     IGGVDRYDTNKQIINKFYNGSKEFYIASGTDLVYSLVGSTIAQKTPIVLVGNGSNKSI
                     LKNASKITAIGNIDDVVIKQCLNITNNVGDSNTGIVKDTNSNNTNKPSNEPSSNNPGL
                     TDKSTVYVTPNGKSYHLTKNCSTLKRSKTINKVTLKQAKSQGKTDPCNICVK"
BASE COUNT        29754 a        14559 c        18689 g        23271 t
ORIGIN      
        1 aaatatatta ataatcagaa aaagcaagta aagtgaggtg gtagtagtga aaaaagctta
       61 taagtttaga atgtatccaa ataaaaaaca gcaagagtta attaacaaaa cttttggatg
      121 ttgtagattt gtatataata agtatcttgc taaaagaata gatgtttata aaaacaataa
      181 agaaactttt acttataaac agtgtagttc tgatttgacc aatttaaaaa aagaattaaa
      241 ttggcttaaa gaacctgata aattttcact acaaaacgcc ttaaaagatt tagataatgc
      301 atataaaaaa ttctttaaag aaaaagcagg atttcctaaa tttaaatcaa agaaaattaa
      361 tagattttca tataaaacta actttacaaa tggaaacatt atgtattgtg gtcaacatat
      421 aaaactacct aaacttggta tggttaaggt aagggataaa caagtaccta aaggtagaat
      481 acttaatgct actatatcta aagagccaag tggtagatat tatgtatcat tatgctgtac
      541 tgatgtagat attgaagcat ttgaaaatac taataatcaa ataggtttag atttaggaat
      601 taaagaattt tgtatctcaa gttgtggaga ctttatagaa aatcctaagt atcttaagaa
      661 gtcattaaac aaacttgcta aattacaaag agaattatcg agaaaaacaa tcggtagttt
      721 aaatagaaat aaagcaagat taaaagttgc aagacttcaa gaacatatag ccaatcaaag
      781 aaaggatttt ctacaaaaac tatctactaa attaataaaa gagaatgata ttatttgtat
      841 agaagattta caagtaaaga atatgattaa aaatcataaa ctatctcgct taatttcaga
      901 tgtatcatgg tcagaattta ttcgtcaatt agaatacaag gctaattggt atggtagaca
      961 aattgtaaaa gtaggtaaat tctttgcaag ttcacaaata tgtaataaat gtgggtataa
     1021 aaacgaggaa gttaaggatt taaatataag agaatggatt tgtcctagtt gtaatgaaac
     1081 tcacgataga gatataaatg caggtatgaa tatactaaaa gaaggattaa gactaataac
     1141 aattcaaaat aaataactta tgtaagaacc gtaggaacta tggggatagc ctgtggagaa
     1201 ttagtaagac atattttagt atgcaaaatt ctatgaagcg ggaaccctgt gactttagtc
     1261 atgggaggtt caggatgaat aaacctgatg gatatatatc ggcaactaca gataaatatg
     1321 acccaacagt gttagattta attgatttat catacttagc ttttgaacca tttcctgttg
     1381 gaaggttaga taaagacaca gagggattat tagttttaac taatgatgga aagctttctc
     1441 atagagtttt atctcctaag aagcatgtgc ctaagactta ttatgcaaaa attgatggag
     1501 tagttactga agaagatgta gaagcttttt tagaaggtgt agtgcttgat gatggatata
     1561 aaactatgcc atcacaatta aatatattaa aaagtgatga tgaatcagaa atagagctta
     1621 caatacatga aggtaagttt caccaagtaa aaagaatgtt tgaaagcgta ggcaaaaaag
     1681 tagtatactt aaaaagattg tctatgggca atttaaaatt ggatgaaagt ttagagttag
     1741 gtgagtatag agagttaaca gatgaagaag taaaattgat tgaagaaaga taataaagat
     1801 actatagtaa attggaggaa tgtctgtgaa gaaaaaagat gttatagaat ttgaagtaga
     1861 taaaatggaa tttggtggta cgtctttaag ccaaattgga gatagagtta tccatatgaa
     1921 gggtggaata agtggtcaaa aggtgagagc tggagttaaa aaagttagaa gtaagaaagc
     1981 agaagttaag atgatagagt tattagagga ctcaccactt gaaactgaag aaacttgcaa
     2041 gcactttaga gagtgtggag gatgtacact attgtctgtg ccatatgaaa aacaattaga
     2101 aataaaagaa aaacaagtta tggatttatt tttaaagcaa gacttatttg gatttcagtt
     2161 tttagggata gaaggaagtc cagaaaacaa atattataga aataagatgg aatatacttt
     2221 tggagatgaa gtaaaaaatg gacctttgac attaggtctt cataaaaaag gaaaacatat
     2281 agatatacaa actgttgagg agtgtatgtt ggttgatgaa gacttctcaa agatattagt
     2341 ttctagtgta gagtttttca atgaaaaaaa gcttccttat tatagaacaa tgaatcataa
     2401 aggatatttg agacatcttg tagttagaaa agggattcat actaatgaga ttatggttaa
     2461 tatagtaact tcttctcaag aagattttga tatgaatgag tttaaagata tgcttttagg
     2521 gattgattta aaagctgagt tggtaggggt acttcatact attaatgacg gtttagcaga
     2581 tgcagtccaa tgtgatgagt taagagtatt gtatggaaga gattatatac aagaagaaat
     2641 tttaggtctt aagtttaaga tttcaccatt ttcgtttttc caaaccaata ctaagggagc
     2701 tgaggttctt tatagtattg ctagggattt tattggagat tacaatgata aggttgtatt
     2761 tgacttgtat agtggtactg ggtctattgg acaggttatg gctggtgctg ctaagaaagt
     2821 gtatggtatt gagatagttg aggaagctgt tgttgctgct aatgagaatg ctaagttgaa
     2881 tgggcttact aattgtgagt ttattgctgg ggatgtggct aaggttgtta aggatttgaa
     2941 ggataagcct gatttgatta ttgttgaccc tcctagacca ggcattcata aggatgctat
     3001 tagggatatt tgtggatttg gtgctaagga gattgtttat atttcttgta atcctaagtc
     3061 tttggttgtt gatttggttg attttaaggg gtatggttat gagattaaga tggttaagtg
     3121 tatggatatg ttcccaaata ctccacattg tgagacggta gcgttattgt ccaaacttga
     3181 tgtcgataag catatagatg ttgaaattaa gctggatgag cttgatttga caagtgcaga
     3241 gagtaaagca acatacgctc aaatcaagga gtatgtatta gaaaaatttg gactaaaagt
     3301 ttctacactt tatattgcac aaattaaaaa gaaatgtggg attgaaatga gagaacatta
     3361 taataaatct aaaaaagata atcaggctat cccacagtgt acgcctgaaa aagaagaagc
     3421 tatcatggat gctttaagac attttaaaat gatttagtag aaatggaggg tatttatgag
     3481 atggatatta aaaattatct tatttccaat tagcttgttg ttaagtatcc tcactgcagt
     3541 tctttcattt ttacttggca taggaacagt aatactgtac ttgctgatga tgttttgtat
     3601 atttggagca atagcatctt ttttacaaaa agaggttacc attggtatag aagccttaat
     3661 tataggattt ttacttagtc cttatgggat accgatggtt ggagcgtcaa ttatagcatt
     3721 attgcaaggt ataaacgaga aaataatgtc agtttaaatc agagaattga taaatcagaa
     3781 gttgtggagg taattttatg cgagtatacg aaaataaaga agaactcaaa actgaaataa
     3841 ataaaacatt tgagaagtat attgcagaat ttgataatat tccagaaagc ttaaaagata
     3901 aaagaattga tgaagttgac agaacgccgg cagaaaatct tgcctatcag gtgggatgga
     3961 caactttagt tcttaaatgg gaagaagatg aaagaaatgg gcttcaagta aagacaccat
     4021 cggatgaatt caaatggaat caacttggtg aattatatca atggttcaca gatacctacg
     4081 cttatttatc attgcaagag ttaaaatcta aattaaatga aaatattaat tctatatatg
     4141 taatgattga ttcattaagt gatgaagaat tatttaaacc acatatgaga aaatgggctg
     4201 atgatgcgac taaaacagca gtgtgggaag tttataagtt tattcatgtg aatactgtag
     4261 ctccttttaa aactttcagg aacaaaatta gaaaatggaa gaaaatagta ttataaatta
     4321 cagacagtaa agttaaaaat ttcataaatg ggttatcaaa ggcgatagaa aaactatcgt
     4381 ctttttattt gaaaaaagaa aggagtgttt gatggtatgt atttatttga tgaacaacct
     4441 atagtcgcga ataaaactct tgcaagagaa ttaggattaa acgaagcgct tgtactacag
     4501 cagatcaatt attggataga aataaataaa aaatcgggaa agaattacca tgatgggaaa
     4561 tactggacat ataattcaat tcgagcatgg caagaaaatg actttgacta tatgagcttt
     4621 gatacagtaa aaagaacttt ttcaaagtta gaaaaagcag gttatctttt agttggaaat
     4681 tacaataagg atccaagaga taaaacaaaa tggtatacaa ttaataatga taaacttgaa
     4741 gaattatata tggatatgga tttaaaaaaa caaaaaatac aaacagaagc attaaaaaat
     4801 tctatgccca atgcattagg gcaaaatgca ccaatggata agggcaaaat cagccaatgc
     4861 aataatgcag attgcactaa tgcacaggtt caaaatgcac taatgcaaaa ggataatttg
     4921 caccaaccat taccagagaa tacaacaaat aatactacaa ataattcagc atatatttcc
     4981 atccatcctt ccatcaaaga aaagaatcaa ttattttcag agaatatgaa gggagggagg
     5041 atagatataa gctataaaaa tatatatcaa aattgtttag aagaattgcg acaccaaaca
     5101 ggatactatg aacatatgag cattgggaat aagagtgtgg ctgaagagtt tgatgaaata
     5161 ttaaagatac tggcagatgt aatggtatta gatgatgaat cttttattac tatcaaccaa
     5221 acaaagatat ctgtacaagt agtaaaggaa agatttagga aacttaattc aggacattta
     5281 gagtacttag taggagtatt aagagaaaat gaatataaga ttagaaatat aagagctttt
     5341 gtactgaccg ccgcttataa tgcaccaagt ggaatagatg cctattattc ggctcttgta
     5401 agctatgata tgagaggagg atattgatgt gatcaatgaa gaaatagcaa gaaagacctt
     5461 aaatatggag ataaatgctg gtaaagttac tggaaaaggt ctattaaact tattaaaaaa
     5521 attgatgaaa gaagcagaga aattcggagg acttgaaaaa ctggtcaatg tcaacggaaa
     5581 tgaagtaaag ctaaaagata tggttaaaaa gggacagctt gaagaaatcc cagtggaaga
     5641 agctgagctt aaagaactta aaaaagagct gaatagatac ggtgttaaat tttcagtgat
     5701 gaaaaataaa gagacaggaa aaaactctgt attctttcaa gccaaagata tgaaagttat
     5761 ggataaagct tttaagcacg ctctttcaaa atcagaaaag aaagcagaaa ggaaagaatc
     5821 tattcacaag aatattgaga agttcgaaga gatggctaaa aactctattt caaaagagaa
     5881 aatcaaaaat aaacaaaagg agcaaagcct atgatagata agatactaaa agacatcaaa
     5941 ggcttattta aggtgcaaga taaggcaaag tttctaaagc agaacattcc ctatcttgca
     6001 tttttctatg ttggcaatat cttttctcat catgtaagag catatactgg tggcgatgta
     6061 atagacaaaa tatttcaagg tatattggaa cttaacacca tgagctttct tccaagtttt
     6121 catccaactg atattctaat aggcgtagga gtagctactt taatcaaatt cattgcctat
     6181 acaaaaggga aaaatgcgaa aaagtttagg cagggaaaag agtatggctc ggcaagatgg
     6241 ggaactagaa aagatattga gccgtacatt gatgaaaagt ttcaaaacaa tatcttgcta
     6301 acgcaaacag aacgattaac tatgaatggt agaccgaaaa atcccaagta tgcaagaaat
     6361 aaaaatgtac ttgtcatagg aggatctgga agtggcaaga caaggtttta tgtcaagccg
     6421 aacctaatgc aaatgcacag tagctattgc gtaacagatc caaagggaac gatagtcctt
     6481 gagtgtggca aaatgcttga agataacgga tatgagataa aaatccttaa taccattaac
     6541 tttaaaaagt ctatgaaata caatccattc gcctacctcc gcagtgaaaa agacatactt
     6601 aaattggtgc agacaatcat tgcaaacact aagggagagg gtgaaaaagc gggggaagat
     6661 ttttgggtca aagccgaaaa actctattat acagctctga ttggatatat cttctatgaa
     6721 gctccaaggg aagaaaagaa ctttgcaaca ctccttgata tgatagacgc ttcagaagtt
     6781 agagaagatg atgaaacata tatgaatcct attgataggt tatttgaagc attggaaaag
     6841 aaagaaccta cgcactttgc agtaaagcaa tataaaaaat acaaacttgc tgccggtgtt
     6901 gtatgctcta aaagacttct taatcaagcg gttgggaagt ctcttagaac acacaaccta
     6961 aaaccgaaga aaggagcgca agttatgaga aaaaacgaga aaatcactgc tctgtatgaa
     7021 cgattgagcc gtgatgactt tggcaaagat gatgaccaac agcgtgagag caattccatt
     7081 tccaatcaaa aggctatgtt ggaggagttc gccgcacggc aggggtttac gaacattgtc
     7141 catttcacgg acgatggcat tagtggtact tgctttgacc gtcccggatt tctggcaatg
     7201 atgaaagagg tggaagccgg gaatgtggag tacctgtgta tcaaggacat gagccgcatg
     7261 ggtcgtgact atctgaaagt cggtcagatt atggaaatcc tgcgtcagcg tggcgtgcga
     7321 cttatcgcca tcaatgacgg cgtggatagt gccagaggtg acgatgattt tacccctttc
     7381 cgcaacatta tgaacgagta ttacgccaga gacaccagcc gtaaaatccg ttccactttc
     7441 cagtccaaag gcaagtccgg caagcacctc acaggcacgg tcatttacgg ctatctctgg
     7501 aacgaagcca gagaccaatg gttggttgac cccgaagccg cagaggtggt aaagcgcatt
     7561 tttgccatga cgattgaggg ctacggtccg tatcagatcg ccagcaaact gaaagaagaa
     7621 aaagtcctca ttccgtccgc ttaccttgcc cagcacggcg agggcgtgaa caaaaacaag
     7681 actttcaaag atgtgtacgg ctggggttct tccaccatct gcaacattct tgaaaagcgt
     7741 gaatatctgg gacacaccat caatttcaag acccgaaagc acttcaagga caagaaaagc
     7801 cattatgtcc cggaggacga atggacgatt tttgagaata cccatgagcc tatcattgac
     7861 cagcagacct ttgaccttgt gcagaaaatc cgtgggaatg tcagacgcta cccggacggc
     7921 tggggcgaag cagctcccct cacaggcttg ctttattgtg ccgattgcgg aggcaagatg
     7981 tatgtccacc gtaccaacaa cggcaagcgc atttctcaat acacctgctc acagtacaca
     8041 aaagttcctt gcgggacact ctgcaagacc cagcaccgta tcaatgaaga tgtggtactg
     8101 tcccttgtct ctgaaatgct gaaagccatt gcagagtatg ccaagcatga cagagccgag
     8161 tttgtccgtg tggtgcagga agcacagtcc agccagcaga cggcagaggt caggaaacag
     8221 cggacacgcc ttgccacagc aaagcagaga gtttccgagc tggaagtcct gctctgcaaa
     8281 atctatgagg acaacatttt aggcaagctg tccgacagca gatacgccac tctggacgct
     8341 caatacgaaa aggaacagtc cgagcttacc gccgaaatct ctgctctgga aaaagctatc
     8401 aagagctatg aaaaacacga aaaggacgct gaccgtttta tcgctctgat tgacaaatat
     8461 gagaacttcg acaagctgac cattgccatg ctcaacgagt ttatcgagaa aatccttgtg
     8521 catgagcgtg accgcaaggg cagtatacag accacacagg aggtcgagat ttacttcaat
     8581 tttgtcggtc gatttgttcc cccggcgttt ggagaagtgg agctgacccc agaggaatta
     8641 gaggaaatcc gcaaacgtga ggaacgcaag gacaggcttc atcagaacta cttgaagcgg
     8701 aaagccagcg gagcgcagaa gcgatgcgag gacaaaatca agaagcggaa aaaggcggaa
     8761 atcgaagcca agaaagccgc cattcgtgcg gaggacattg caaagggagt gttcgtccct
     8821 gtcagcagtt taccgcagag agagccgatg aaaggagtac aaacagcatg aatatcactt
     8881 acacacagaa cggcgattat cttatcccaa acatcattat ccgcaagacc aagcccctcg
     8941 gacactacgg cagacttcgc aaggcgtatc tggaaatgca ccgtccgata ctgttcaatg
     9001 agctggtgct atccgacaag ctctttgagc attgcgccga gattgacgaa acagcacgaa
     9061 accgcatgga gctgatcgtg cggtcactgg cagagcaaaa cggcgtgacc gagcaactga
     9121 aagccgaaaa ccaaatggaa tgggtgcggc agatgaacgc ttgcaaggca caggcagaag
     9181 atatcgtgaa gtttgagctg atatatgatt gataccgaga ccgcccggac gaaaaatgtt
     9241 cgggcggtca aaatttcttg aaggaattgt tactttttct caaagctcat tgacaagaaa
     9301 acttggcgcg agtataatta agatatgcaa agaaaacttg gcgattggag gttatagtat
     9361 gaacaaagat gaagtcctca aaatgagccg tgaagaaaac gaaggcagac atgacgagcg
     9421 tgaaataatt gcatttggaa cagcttcaag agcgggaatg cttgtaggcg cattggtgtg
     9481 tgtattgctg gtatttgcaa gcgagttgct ttttcatatt ccagaaatcg gactggttgg
     9541 ctggctcgta tattttgcaa tgcaaggcag tagcaatatt gtactgtata aggacttgaa
     9601 aaaccgccga aatctcattt ggggcattat tgaaattgct tttgctgttg cgtttgccgt
     9661 ggtgcttgta attagaagcg tggtatgact atgaacgaaa agctgatatt aaaaaacaat
     9721 ctcaaagaat gtcgtagcga aaaaggctta tctcaacaac aactggcaga tatggtcggt
     9781 gtatctcgca atacgattag ttcaatcgaa acagggcaat tcaatccgac tgcaaagctg
     9841 gcgttgattt tgtgcatagc cttagataaa aaatttgaag aattgttctt cttctaaaag
     9901 aacataagga ggatatttat ggataagcaa acaacaggaa ttgtgatcgc tgcaacaaaa
     9961 cagtggtggt taaaggtcaa cagaaaaccc atgagattgc acacactgga tggtgctgaa
    10021 tttccttaca tcattaaaat tgaatacacc gtagatggaa atgcgtacac aaaaagaaaa
    10081 tggattaacg ccggtgaccc tgttcccaag gtagggagta atgttcaagt gatgtataat
    10141 tccgacaagc catccaaagc gagaatattg taaaagcatt cagatgacga gaacgaaaaa
    10201 tccctttagg aactaaaggg cgcacttcta tactctccta tcgggagtat cgtgcgtcct
    10261 gcggagcttc attctccgtc agcagaagaa aactgcttga ccgagaatgt tgcgtcactt
    10321 cgttccgtca aggtggctac acccccttgc gccgctaaag cggctatccc ttgtggactt
    10381 gccgtccgca aacagcataa aatgctgttc gccgccagca agtttgaaaa actgaatacc
    10441 gaaaaactga ccgttgactg gtcgcactat gcgaaaagtt gacggtcttt ttttatcccc
    10501 aaaatcaaaa aaggaggtca atcccaatgg caaaaccgaa aaccctcgac cagctccgag
    10561 ccgaaaagga acgagctgag acgcagcttg cacaggaaca gcacaagctg gagcgtctgg
    10621 agaaccgaaa gaagtatctg gagaaaggcg aacgcaccaa gcgcacccac cgcctttgca
    10681 atctgggcgg aacgattgag agccttgccc cggaggtcaa agatctcaca cgcaccgaaa
    10741 tgacagagct gatggaacac atcttttccc tgtccgaagt ccagcgagcc gtccgtcaca
    10801 tggcgattac ccacatcagc caagcaaaca gagaaaggga gttgaaagcc gatggcacta
    10861 tttcatctga gcgtcacgca gactaagcga agcgcaggac agtccgctat cgcttctgcc
    10921 gcctatcgtt ccggcgaaaa gctgtatagc gagtattacg gcgaatacag cgactacacc
    10981 agaaagggcg gcgtgatctg ctctgacatt ctcctgccgt cccatgcacc gcccgaatac
    11041 gcagaccgcc agacgctatg gaacgccgtg gaaaaagccg agcgtggaaa gaacgcccag
    11101 cttgcataca gctttgacat tgccttgcag aatgaatttt cccttgagga aaacatcgct
    11161 cttgcaaggc aatttttatt ggagaacttt gtgagccggg gcatggtggt tgacttcgcc
    11221 gtacaccagc cagaccggga ggacggcggc atacccaatc cccattttca tgtgctttgc
    11281 cccatccgtc ccatcgagca gaacggcaaa tggggctgta agcagcaccg agtttacgag
    11341 ctggacgagg acggcaaccg tatccgggac gcagatggaa actttgtgtt taacgccgtt
    11401 cccactaccg actggggcag tcccgaaacg ctggaatact ggcggcagac atgggcggag
    11461 ctatgcaacg ctaagtttgc ggaaaaggag cttgacgttc gtatcgacca ccgaagctat
    11521 gagcgtcagg gcgtggagct tcttcccacc gtccacgagg gcgcaaccgt ccgggcaatg
    11581 gagaagaaag gcatacgcac cgaaaagggc gagttcaacc gctggatcaa ggcaaccaat
    11641 gccgttatcc gggacatcaa gaagaaaatc gctctcctgt tcgattggat tgccgaagca
    11701 aaagcagagc ttgccaagcc gcaggccccc gaccttgttt ctctgctgaa cgcctattac
    11761 acccagcgca gagccggggc gtattcgcag aaaggcaagg tcagcaatct aaaggagatg
    11821 aacgagactt tcaattatct ccgggcaaac ggcatttact cccttgaaga tttggaaagc
    11881 cgtgtcagcg aacacagtgc tgccacagag agcttgaaga aaacgctgga cgaacagacc
    11941 gcccgaatga aagcaattaa gcagctctat gacagctctg ctgctttcca gagcttgaag
    12001 cctgtctatg acggcttgca gaaaatcaag tttgagaagc ccagagccaa gtacaaggct
    12061 gaacacgccg acgaactgaa aatgttctac gccgccagac gaaagctgac cggagagttc
    12121 ccggacggca aggtggatat gaaaaagctg accgaagagt atgacgagct ggaacaggcg
    12181 cacaacacca cctatggcga gtttaagacc gtcagagacg atttacaccg tctttggaag
    12241 gtcaagtcat gtgtagatac tgccgcccga tttaacgagc gcacagagga acaaaagctc
    12301 caaaatcgac ccaaaacacg acacaaaaag gaggatatga cacgatgaaa aaatacgact
    12361 ggaagaacga gtacaacccc atccgcagaa cggcaaaacg ccttttgagc ggagaagctt
    12421 tgaccgatgg cagaccgctt tccgacctga tcgagctgga cgaacacacc cggaaatgcc
    12481 ttgcctttga agccaccctc accgaccgcc agattgatat gttgcaggag ctgggctatc
    12541 tgcctgttac cacaagacag tacggcgagg aatgtgcaaa ctgcccctac acgttggacg
    12601 agcgtgagca tggcgtttgc agacgatgcg atgttttcaa gaagtaagga ggacgctcat
    12661 gcagtacacc cgagcgcaga ttgaccgggc aaacgccgtc agtctggaag attttctccg
    12721 cacacaggga gagacgctta tcaaaagcgg acgagaatac cgctggaaag aacatgacag
    12781 cctgaccgtc cggggaagca agtggtttcg ccacagccag agcaagggcg gctatcccat
    12841 tgattttgtc atggagtttt acggcaagtc ctttcccgaa gctgtccagc tcctgaccgg
    12901 cgaaagcggc gaggggcaga ccgaagccag cacagcaccg cccacagcgt ttcacttgcc
    12961 attgcacaac aggacagccg acagagcaat ccaatatctc acagaaagcc gaggtctcaa
    13021 caaaacgctt gttgaggctt ttcttctttc cggggatatt tacgaggacg caaagcggca
    13081 caatgttgtg tttgtcggca gagacagaag cggtacgccg agatacgccc atgtgcgagg
    13141 aacggcagac ccattcaggc aggacattgc cgggtctgac aagtcctatc cgttccgcta
    13201 tgagggaaac ggcaaccagc tctttgtctt tgaagcaccg attgacctgt tatcattcat
    13261 ctgcctttat ccgcaggact ggcagaaaag aaattatctt gccctgggcg gcgtttcggg
    13321 caaagctctt gaccgttttc tttctgaacg caaggacacc caaaaagtgt tcctctgcct
    13381 tgacagcgac accgcaggaa gcgaagcctg tacccgactg gcacagtcca ttcccagcga
    13441 gatcgccgtc attcgccttg tcccggcaag gaaagactgg aacgatgttc tccgtcagca
    13501 aggggacatt cccagccgca aatttatagc ggaaacaatc acgctgcgag agctgcccac
    13561 cgcccagcct gtcccaatgc tacgcatggc agatgtggag ctgacgagcg tggattggct
    13621 atggtttccg tatatcccct ttggaaagct gacgatcata cagggcaacc ccggcgaggg
    13681 taagacctac tttgccatgc gtcttgcggc ggcttgcacc aaccgaaagc ctttacccgg
    13741 tatggagaaa cttgagcctt tcaatatcat ctatcagacc gcagaggacg gcttgggcga
    13801 taccgtcaag ccccggctga tggaagctga cgcagacctt gaacgagtgc ttgtcattga
    13861 cgatagagac acgccgctga cccttgccga tgagcgcata gcaagggcaa tccgtgagaa
    13921 caacgcaagg ctggttatta tcgacccggt acaggcgttt ctgggcgcag atgtagacat
    13981 gaaccgggca aatgaagtcc gtcccatctt ccgcagtctg ggagacattg cacaggctac
    14041 cgggtgcgct atcgtgctga tcggacacct caacaaagcc gcaggaacgc aaagtaccta
    14101 ccggggattg gggtctatcg acattacagc ggcagtccgc agtctgctgt ttatcggcaa
    14161 gctgaaagac agtcccacaa cgagggtgct tatccatgag aaaagctccc ttgcgccgcc
    14221 cggacagtcc cttgcctttt ctctgggaga cgagcaaggc tttgagtgga taggggctta
    14281 tgacattacc gctgacgaac ttcttgccgg gacagacacc gccaagaccg aaagcaagac
    14341 cgcacaggcg caaatgctca ttttggaact gctggcagat ggaaagcgta tgccgagcgc
    14401 agagctggaa aagacggtca atgagcgtgg gatttcctca cgcaccatga gaacggcaaa
    14461 gagccgtatc ggggacagac ttgtgaccga gaaagacggc acagcatggg tctgctatct
    14521 ccgagactga caacaggaac acggcaagcg tggcaaaggc ggcaaggttt ttatagatac
    14581 cttaatgttg ccgccttgcc ttgttccctc ataccgacac cgcccgatga tgaacagtca
    14641 ccgggcattt ttcatttaca ggaggatttt gaatgaacaa taccgcaacc aacaccgcta
    14701 cttgcccgac tgtcagaaag cagatcggca aaacaaccta tatcgtccgt gtgcatttca
    14761 gccagaccgc aaaagagaca atggaggaca aaatcaagcg tctgctccgt gaggaagtcc
    14821 gcaaaatgta acttcttttt gaatttgatg aaaaagtcct tgacttttcg tctctggaaa
    14881 aacagcgaaa tctattctta tctcttgtgg tgcaaggctt gctccatttg atattaggga
    14941 acttagggac ttgatggggg aagatgaact tgagcttgat acactcggag atagaaaaac
    15001 ggcactcttt gttattatct ctgatacaga tgataccttt aactttgtag tgtctattat
    15061 gtattcgcag ttatttaatc tcttatgtga taaggcagat gatgtatatg gcggacgatt
    15121 acctgtccat gtgaggtgtc tacttgatga gttcgctaat atcggcttaa ttcctaagtt
    15181 tgagaagtta attgcaacaa tccgttcaag agagatttca gcaagtataa tacttcaagc
    15241 acaatctcag ttaaaagcaa tctacaaaga taatgcagat acaattgtag gtaattgtga
    15301 tagcaccttg tttcttggcg ggaaggaaaa gactacatta aaagagcttt ctgaaacact
    15361 tggaaaggaa acgatagacc tttacaatac ctcggaaaca agaagcaatc aaaagagctt
    15421 tggattgaat tatcaaaaga caggaaagga gctaatgagc caagatgaga taacagtaat
    15481 ggacggtgga aaatgtatat ttcagcttag aggtgttcga ccttttctat cagataaata
    15541 cgatattaca aagcataaga attacaagct ccttgaggac tatgacaaga ggaatttgtt
    15601 tgatatagag agctacatta agcgtaaagg gaaagtgaaa atgaatagac atactgtaat
    15661 tacaagattg taacattttt cttatcacta tatgtagatg tataaatttg gtataatgtt
    15721 gtttaaataa atgatatttt atgtatgaaa cctataatat ttgtactaat actgtggggt
    15781 aggaaaagaa aacttcctat atactaaaat caaccgaaag aaaggaggaa attagaatga
    15841 atattatcaa gtcatttaac agtacaattg attatcttga aacagttctt gatgatgaaa
    15901 ttgatgaaaa gaaagtaact agaatatccg gatattctta ttcgatgttc agtcgcttgt
    15961 tttctattct aactgaaatg acactttcag aatatttaag aagcagaaga ttaacggaag
    16021 ctgcgattat attaagagat acagatgaaa aaattattga tgttgcattc aaatttggat
    16081 atgagtcatc agattcgttt gggactgctt ttaagaattt tcatggattt actccttctg
    16141 aggtgagaat tggaaaaccg tttaaattag tttccagggc gcaattagca ttaagtgtaa
    16201 gaggaggaag aagtatgaat ataaggattc aaaagaaaaa aagcatttac agttgcaggt
    16261 gtaaatcaac aaaacatcaa ttcatcaata tgtgcaagcc tatggcaaaa actattttca
    16321 aaggtatctc atgaagaaat gttaaactta gggaccggag aagttgtagg agtatgtcat
    16381 gatatattaa gtcttgataa tcatgatgtt ttagatagta acacgataaa ttatatggca
    16441 ggatatattg ttacagatgt agataaggca agaagtatgg gactagatat tttagaaatt
    16501 ggagaaacag agtatgcggt tgtagagtta acaggtagtg ttccagagtg tattcataac
    16561 ggttggaagt atgcgatgga agtgttcttt cccgagcatg gatatgttca ttcaggaaag
    16621 ccggactttg aatattatta tgaaggagat atgtatagta aagactacaa aatggaactt
    16681 tggattcctg tatgtaaatc ttaacaaatt tcagtctata gttttaaaac aaaatatata
    16741 aaatacaagg tgatagaaag taaaagctat cgcctttttt atgtaaaaat ttaaggagat
    16801 agaatgataa ggataagaag tcctacttaa aggaaaatca aatagcaaga gtataagcga
    16861 ttggcaaagt atgaagtccg gtcgcttttt taaaagaaaa ggagaagttt taatgaagca
    16921 agaaatgatt aatattaatg ccaacttgtt ggcagaaccg actttctcaa aatttgaaaa
    16981 agagggagaa aaagtggaag ttgcaaactt cacgcttgta aaaaagtatg ggaagggcaa
    17041 ggaatatatc aattgtgcag cctatggtga aaaggcgaaa acagtaaaag cctttgaaaa
    17101 aggcgatttg atccatatct ttggttactt taagaagcgt gaaaaagacg gaaagactta
    17161 caaaaacttt gttgtaaagt catataacaa gattgaaaag aaagaagaaa acgaggagga
    17221 ataaatcatg gaatttttta cacatgcggt aaatgtatta aagattttag ttatggcagt
    17281 aggtgcagga cttggagcat ggggcgttat taacctgatg gaaggttatg gtaatgacaa
    17341 tccgggtgca aatgctcatg tgccataaag taacacataa gaattgatag acagcagccc
    17401 actattccgc agcaacagcg agttgcttgt taaaacccca ttttgaagtt ataatgagta
    17461 aaaacaacct ttaggaggga cgggcatgga aatacaaggt attcttaaaa atttacgcga
    17521 gaaaaacaac cttacgcaag aacaactggc tgaacgtgtg caagttaccc gacaggcggt
    17581 tagtcgctgg gaaacaggcg aaacgcagcc aaatacagat accttaaaac tgttatcacg
    17641 agtttttgat gtgtcaatca atacgcttct gggttctcca cggcaacttg tttgtcagtg
    17701 ttgcggaatg ccattgaatg aagatggtct gattagccga gaaccagacg gaagctataa
    17761 cgaggactat tgtaaatggt gctatgctaa tgggaaattt gcatatcaat ccaaggaatc
    17821 tttattggat tatctggttg cgaatatgcc aaatcccgat aacgcagatg aagaaaccag
    17881 acgtaatcag tttgatttgt acctttcgca attaaagcac tggaaataac tatattgtca
    17941 tagcaaagcc agccgagcca gtcaacggtc aagatgaacg gcgcgtaccg cgccgccgtt
    18001 gacagccccg cccgcctttg ctggtaggca atcaaggggc gacagcaagg agtgctgccg
    18061 ccccgcacta ttatcagaga gggggaattt ccatgaccga agatgaagcc tacaaagtgc
    18121 atatccagta cacctttaac gccttttgca aggttgtcat tcgtcacgcc gccatagata
    18181 taattttgaa gctgcgccgg aggtgggaac gggaagtttc ccttgactat ctgatgaatg
    18241 agaagtttgt ccagcttgcc gagccggagc agcttgaaga atatcttttt accgcctgcg
    18301 gccagaccgc cgttctgtac catgcggaac tcgccgccgc ccttgccctt ttgccagagc
    18361 agacacagga agaaattttc cgttactatt tcctgcgcca gccgcagcgg gtgatcggcg
    18421 tacatatcgg acggacacgc agcacagcag ggcggcatat ccagcttgcc ttgaagcggc
    18481 tccggcggct catggaggga aaacgctatg agtaaacttc tcccctatga aacaatcgtc
    18541 aaggcgcatg agggcgaccc ggacgcaatc gacaccgttc tttcccacta cgtcggatat
    18601 atccgatact gttccaaagt acacgggaaa gtcagcgccg aggttgagga acatataaaa
    18661 cagaagctaa tcgccgcgct gttcaaattt cgctttgacc gataagcaaa gaacgaacac
    18721 cgactgtcat tagcgatttt caccagcaag tttacaattc atctattccc ataagtaagc
    18781 ggtcatagta taataaagcc atcgacaaat agtaattata ttttttgaaa gaattctata
    18841 gagtaaaaat attttgtctt ctatgtttta gaaaatgtgc aacagtcgtt ccgtgagact
    18901 ggtatgtgcc caaaaaaaat ggtctatgca agaaaataga tacaacaata gtgagcaaat
    18961 gattcaggta aagatcagat atataatatt ttaaagtaat tgtacatttt ttaagaaatt
    19021 acaatggtca ttcatcgtgt ttgaaagaat aggttattgg cgcactttac ttatatatcg
    19081 ttttacaggg gctgtttttt atgcaacgtt agggcttact cgtatatagt tatgaaaata
    19141 aatagagaat gaggtgcgat tagatgaaaa atcttggata ttcacaaaat tttttggtta
    19201 ataaaaagtt ggtagaacgt ttaataagca agtcaaatat tgatgtaaca gattatgtta
    19261 ttgaaattgg tcctggaaaa gggataataa cagatgtact ttctcagcat gctggagagg
    19321 ttgttgcagt tgaatatgat caagaattat ataataattt agtaagatat cacagtcatg
    19381 ataatgttac atatatattt ggtgattttt taaaatataa gttgccttta aatcgcagat
    19441 ataaaatatt ctccaatatt ccttttcaga taacagcgga tattattagg aaattaacag
    19501 acgatgtaaa tccaccatca gatataaata tcataattca aagagaggca gctaaaaaga
    19561 attgcggtat tccattacaa aaatatgaag gttttcgtgc tgctataata aaggcacaat
    19621 ataaggtaga aattacacat agttttaaaa ggagcgattt ttttccttcg cctaatgttg
    19681 atacagttat gcttcatatg cagttatggg atgatagatt gagtggtgat gatcttcaaa
    19741 attataaaga tttagtggct tttttttaca ctaatattaa aggagagact gctaaagaga
    19801 gactttctat tttattttct aatgagcaaa tcaaaagatt gggaaaagcc aatagaatta
    19861 gtctttcaaa ttcatatacg ttaatcacgg ccgagcagtg gatgaatatt tattattact
    19921 ccaaaattgg attgacagat gaaaagaaaa aaaagtttca agatgcatat aaaaaacttc
    19981 aaaaaatgaa taaaaaattg aaaaagcaaa acagaacatc tttacgaaag tctagtagca
    20041 ataagaaatg tagaacaagg attgatactt ctaatggtac aaaaaataga tagtattgat
    20101 aatttctata aaaatgtgca tggtagtgcg aaaatctgag aagagaattt aatgctggat
    20161 tttgttgata gtagatttca atgagttaac cataaaagta tattagaaag taaaaacaac
    20221 aggggtagta gaatatattt gttagataca agaaggagag attgaattta aggtcatcta
    20281 atactcgcgg aatagtgata ataatggtaa gctgtattga aaaaaaacaa aataactaac
    20341 attatgggat tatttcggta tgaattgatg ttttatcatc ttacatagaa tcagaaattt
    20401 gaaaaattaa aagaggacta agtttatgag aaccctgttc ttaacttcaa gtggcttaaa
    20461 cgaaaagact atggtattat tttggaaatg tatcggaaaa gaaccgatta acacaaaagc
    20521 gattttggtt ccatccgccg cagttggaaa tgatggtgcg cgagaaggaa tcattgtatg
    20581 tatggagcgc cttatgaata tgggaatacc tatgaataat attcttatat ataatcttgc
    20641 gtttttactt tccgatggat ataaaagaac atattcaagt tatattagtg acatacctgt
    20701 accgtttcgc ttaatgagtg ttcaagaact cactcaatat gacatgattg ctttttgtgg
    20761 aggaaatgcc catacgcttc ttagcgaaat aaacagaaca ggattttcga agcccctaaa
    20821 gcaagcaata gagaatggat tggtgtatct gggaattagc gcaggtagta tgattgctgc
    20881 cggaaatttt tccgatggat taggatattt agcaaatccg ttgataccac acgctgaaaa
    20941 agagagccct tttggagata tatcgaagaa cgatttgatt gaattggctg acggtcaaac
    21001 tgtattgatt aaagggaatc gacaggaaat tatttaataa attgaatact gttgtttttt
    21061 tatcttcggg gcaccctgac ccgaactttc aaaactgaat atcagccgcc caccagcggc
    21121 cagcgaaagc agtaagtctg aaaaagattt gctgcttttt ttgtgtccat ttggcaaaat
    21181 cgaaaatcac ccgtagtagg taggtgaagc cagaaaaaac tgcctgctcc gtttggcaaa
    21241 tttctaagag cggtggtgtg gggagtgaaa ggaaaaattc tctgccccgc cggttgccaa
    21301 aatcctgttg cccggattac taaggcaaaa gaaattttga aaaatccccg tccagcgggt
    21361 agctggcggc ctttgaaatg tatctttagc ggaaagggaa aaagccgcag ccagcgggca
    21421 gcaaagcccc gttccggcgt catattggcg aaaggaaaaa aggccgccca cagcgggccg
    21481 caagccctgt cctgtccgtt ctatgtacgg aaaaaccaga caggggcagt ctgcggcagt
    21541 cttagagcaa gtttctacca cggtgccaaa atccgcaatt ctgcggtttt gtccctcgcg
    21601 ggaaacttgt tggggagtgc cttccccaaa ccctgctatg gcggcttgcg ccgcaaaaag
    21661 atttccgttc ggcaggccgt ttttttgcga gaactgcccc ggaaatacct aacagaccag
    21721 cctatttttt gtgataagtg aaaggaggtt tacagcccat gccgaagcga tacaacacgc
    21781 cccaccgcag ccgcgttgtg aaaacccggc tgtccgaaga tgaatacgcc gacttcacag
    21841 cgcggcttgc gccctatggt atcagccagt ccgaatttct ccggcaggcg atacggcgca
    21901 ccgccatacg cccaattatc catgtgtcgg cggtcaatga cgagctgctt tccgctgtcg
    21961 ggaaactcac agccgagtac ggcaggatcg gcggcaacct caaccagatt gcccggtatc
    22021 tgaacgagta cggcgtaccc tacaatgcgc tgtccggcga ggtacgcgcc gccatagccg
    22081 accttgccgc cctcaagtat gaaatcttac agaaagtagg tgacgcggtt ggcaacactc
    22141 aagcatatca actctaaaaa cgccgactac ggagccgccg aacaatacct gctttttgag
    22201 catgacgagt ttaccatgaa gcccgtcctt gatgaaaccg gcaggcttat cccccgcgag
    22261 gactaccggc tgtccacgct gaactgcggc ggggaggatt ttgccgttgc ctgtatgcgg
    22321 gcaaatctcc gctatgagaa aaaccaacgg cgggaagatg tgaaaagtca ccactatatc
    22381 atcagctttg acccacggga cggcccggac aacggcctga ccgtagaccg ggcgcaggaa
    22441 ttgggcgaga agttttgcgc cgagcatttc cccggccacc aagcccttgt atgcacccac
    22501 ccggacgggc ataaccacag cggcaatatc catgtgcata tcgttatcaa ttcgctgcgg
    22561 atagaggaag tcccgttcct gccctacatg gacaggccag ccgacacgaa agccgggtgc
    22621 aagcaccgct gtaccgacgc ggccttgcgc tacttcaagt ccgaagtcat ggagatgtgc
    22681 caccgggagg ggctttacca aatcgacctc ttgaacggca gcaagaaccg cgtcacagac
    22741 cgggagtatt gggcgcagaa aaagggacag gccgcgctgg acaagcagaa cgcccccatg
    22801 attgcaggcg gtatcacgcc ccggcagacc aagtttgaaa cgaacaaaga gaagctgcgg
    22861 cagaccatac gcgccgcgct gtctgcggcg gcctcattcg aggatttttc ctctctgctg
    22921 ctgcgggagg gcgtgaccgt caaggagagc cgggggcggc ttagttacct cacgccggac
    22981 aggacaaagc ccatttccgc ccgcaagctg ggcgacgatt ttgaccgcgc cgctgtcctt
    23041 gccgttttgg agcagaacgc cgccagagcc gcagaaaagg ccgcggctat atccagcacc
    23101 ccgccctcta tccaagacca actgcgggca gacagagccg cccgaaccgc cccgaatgtg
    23161 cagcggcttg tggacattga gcagaagaaa gccgagggga aaggccgggg ctatgaacgc
    23221 tgggcgacca tgcacaacct caaacagatg gccgcgacgc tgaatgtgta tcaggaatac
    23281 ggcttcactt ccccggagca gttagaagcc gccgttgata ccgcctatca ggaaatgcgc
    23341 cagaccagcg gcgaactgaa agcactggaa acgaagctgc aagggaaaaa ggaattgcag
    23401 cgacaggtgc tggcctacgc caagaccaaa cccgtccgcg acggcctgaa agcgcagaaa
    23461 tcccccaaag cccgcgaagc ataccggcag gcgcatgaga gcgattttat catagccgac
    23521 gcagccgccc ggtatttcaa ggcacatggc attaccaggc tgcccgcccg gaaagcgttg
    23581 caggacgaga tcgagcagct tgtttccaag aaatccggcc tgtataacac ctatcacgca
    23641 cagaaacagc ggtttaagga gttgcagacc gtcaagcgga acatcgacca gattttgcgc
    23701 cgggaggaac cccgccgcag aaaggagcag agccatgagc gataacctgc cccgcatgga
    23761 ctaccgccag caccggcggg cgcggcggct ggtgcatgag tgctgtaact acgacgaggg
    23821 gaactgcctg ctgttggacg acggggagcc ttgcgtatgc gtccagagca tttccttttc
    23881 cctcatgtgc cgctggttcc gtgtggctgt cctgcccctt gacggggagc tggccgcagc
    23941 cctcttgtac cgggggagcc ggaaacggtg cgccgtctgc ggcgcggcct ttgtccccaa
    24001 atccaaccgg ggaaaatact gccccggctg cgccggacgc atgaagaaaa tcaaagccgc
    24061 cgagcgaaag cagaaacaaa ggcagaaatg tcacgcttta gagcctttca aacccgcata
    24121 aatcaaggct tttttcgtgg gtgtcaaagg gtacgcgata catttatcct tttcccccgg
    24181 aaacagcgtt ctaaatgcgt acaaaccgtc aaaatcaacc cgaacacagg gaggtgatcc
    24241 tatcgctgat tatatcatgg cagacacgaa gctgcccgcc tatctgccct atccccgttt
    24301 cctgctgaaa atggagattt cccagaccgc caagctgctc tatgcgctgc tgttagaccg
    24361 ctccaccctc tcccagaaga acaagtggca ggacggcgag ggcaggattt atattatcta
    24421 tcccatcgcg gagatagccg aaatactgga taaaggcagc accaccataa agggggcgct
    24481 gaatgaactg gacatggcgg ggctgctgga acgggaacgg ggcggcttct ccgcaccgaa
    24541 ccgactgtat gtgaaagtgc cgcgtgtgcc acaggtacag ttttccgacc aactgatggc
    24601 cggaaaaccg accctcatag agccggaaaa ccgaccaact gatggtcaga aaaccgacct
    24661 tatgatggtc ggaaaaccgt cccctaacca aactactata aacaacctta cagagaacca
    24721 aacaaaggga gtgagtgggg agccgcccgc gccctatggc cgataccaga atatttttct
    24781 gtcacagacc gaatacgacg agttgcaggc agagtacccc gacaggctgg aacggttcat
    24841 cgaggaaatg agccgctacc ttgccgccag cgggaaaagc taccagaact atgccgccgc
    24901 ccttcggata tgggcgggga acgacaaaaa gggcgccccg aaaaagggca tccctgacta
    24961 ctcatgcaag gagggcgaga gtttatgaag aacgaaatca acgctgtttt ggagaatatg
    25021 acgactgcca ccccggagcc ggaggactac accggcgagg acggtttact gtactgcggc
    25081 aggtgccgca agccgaaaga agcctatttt gcgccggata aggccgctat ctttggccgc
    25141 gaccgccacc cggcagagtg cgactgccag agagccgccc gcgaggaacg ggaggccgcc
    25201 gagaagcggc gcaggcactt tgacaccgtg gaggaactga aacgccgggg ctttaccgac
    25261 cccaccatgc gggactggac ttttgagaac gacaacggca ggaacccgca ggccgggatt
    25321 gcccgccggt atgtggaaca ctgggaggat atgcgagccg acaatatcgg ctgcctgttc
    25381 tggggcggcg tgggaaccgg gaaaagctac cttgcgggct gtatcgcaaa cgcccttatg
    25441 gagaaagaaa tccccgtcca catgacgaat tttgccctta tcctcaacga ccttgccgcc
    25501 agctttgaga accgcaacga gtacatttcc cgcctttgcc gctatccgct gcttatcatt
    25561 gacgacttcg gcatggagcg cggcaccgag tacgggctgg aacaggtttt caatgtgatt
    25621 gacagccgtt accgcagcgg caagccgctg atcgtcacga ccaacctcac gctggacgac
    25681 ctgcggaacc cggaggacac cgcccattcc cggatttatg accgcctgct ttccatgtgc
    25741 gtcccggtac gctttaccgg cgacaacttc cggcaggaag ccgcgcagcg gaaaatggag
    25801 agcatgaaga aactgattac cgactgaaag gagtattgcc tatgacagat aacaagcagc
    25861 acgacacccg caccgcccgc cgccccgact gcgtgacgga aatccgcatg ggcaattccg
    25921 tccttgtcgt gtccggctat ttcaagaaag acactaccac caccgccgcc gacaaaatgg
    25981 cgcgggtact ggaagcggaa gccgctgcta cacaaaaacc ggcaatttga caaaccataa
    26041 agaagcagat tttacacttt tgccgctata cagcccccgc tattccgtgg tacaataaag
    26101 ctacggaata gtggggctgg ctgtcggaaa cggaggattt tatgttaaga caagccaccc
    26161 aaaacctcat taccgccctt tatccgagat tgtcccatga ggacgaattg caaggtgaga
    26221 gtaattccat atcgaaccaa aaaaggatac tcgaagcctt tgcaaaacag aatggcttta
    26281 ccaacctgcg ctggtacacc gacgacggct tttccggcgc gaacttccag cgccccggtt
    26341 ttcaagccat gcttgcagac attgaagccg ggaaagtggg tacggtcatc gtcaaggaca
    26401 tgagccggtt agggcgaaac tacttgcagg tagggtttta cacggaaatg ctgttccctc
    26461 aaaagggagt gcgttttatc gctgtcaacg acaatgtgga cagcgcaaac ggcggcatgg
    26521 acaacgattt tacccctctg cgaaatctgt tcaacgaatg gctggtgaga gatacgagca
    26581 agaaaatcaa agcagtaaaa cgagcaaaag gcatgagcgg caagcccgtt accagcaagc
    26641 cggtctatgg ctatctcatg gacgaggacg agaactatct cgttgacgag gaaaccgcgc
    26701 cggttgtcca gcagatatac cagctttgcc ttgccgggaa tggcccgacc aagattgccc
    26761 gtatgcttac ggagcagcaa atccctacgc cgggaacgct ggaataccgc aggacgggca
    26821 gcacccgccg ctaccacccc ggctatgagt gcaaatgggc gacgaacacc gttgttcata
    26881 tcctcgaaaa ccgggagtac accggctgtc tggtaaactt caagacggag aagccctctt
    26941 acaaggtcaa gcacagtgta gagaaccccg tagagaagca ggccattttt gagaaccacc
    27001 atgagcctat catagaccgg gaaacatggg agcgcgtgca ggagttacgc aagcagcgca
    27061 aacgcccgaa ccgctatgat gaagtggggc tgttctccgg tatgctgttc tgcgccgact
    27121 gcggtcatgt gatgtaccag cagcggtatc agaacaagaa ccgcaagcag gactgttaca
    27181 tctgcggcag ctacaagaag cgcacccgcg actgtacggc gcactttatc cgcaccgacc
    27241 tgttgaccgc cggtgtcctc tccaatctcc ggcaagtgac cgaatacgcc gccaagcacg
    27301 agagccgctt tgtgaagctg cttatccagc agaacgagat cggcggcaag agaaagaccg
    27361 ccgcagccac caagcagctt gaacaggcgc aggagcgcat tgccgaagtg agccgcatta
    27421 tcaagcggct gtatgaggac aatgtgaacg gcaaaatcag cgacgagcgt ttcatggaac
    27481 tgtcggcaga ctatgagcag gaacagcggg aactgaaaga ccgcgccgcc gctttgcagg
    27541 ccgaactgga caagtcgcag gccgccaccg tcaacgcgga aaagtttatg ggtatcgtcc
    27601 gaaagcacct tgcctttgag gaacttaccc ccaccctctt gcgggaaatg attgagaaaa
    27661 tcgtcgtgca tgagtgtagc tatgacgaga acggcacccg caggcaggac attgagattt
    27721 attacagctt tgtcggcaag attgacttgc ccgaagccta acgcccgacc tatccgacac
    27781 aatggccaag tgccggatag gaacggcaaa attttttaca cttctattac ttctttatca
    27841 cacataagca aaaagtcaag gaatcaagca gcttatggct aattagatgt aacaaaagct
    27901 aagaaaggaa aagcacaatc cagacggaac cggcggtgtg gtcaagaaat attgtggaaa
    27961 cgcaagattt ctgctataat gggtgcagaa cacttaacga tggaaagagg gacgggaagc
    28021 ctatgaacat cagctatcag ccattatgga acactttgaa agagcgtggc atgagaaaag
    28081 aggacttgcg gcttgccgcc ggtctgacaa ccaacatgat tgccaacatg ggcaagggaa
    28141 aacacatcag catggaaacc ctactgagaa tttgtaatgc tctgaattgt gatatttcag
    28201 atgtgattga attggagcag gatgaaagta ccgctgaaaa agggtgacaa atacaaagtt
    28261 gaaggagcgt gtgattacat gaaaaagatt tttctgaaaa taatgcttgg ggttgttctc
    28321 gtttgcgctt tgtttgttgg atttctttat gccaacaatg atattggtgt gacttcaacc
    28381 aatttagaag cggatattcg ctcgtcgcaa aagattaagg atgattggac agttgatgga
    28441 agtgtttccg acacgatggc tgcatatatt tcttatcccc aggatatgag tgaccacact
    28501 ttttccgtct atgttaatcg tccagggctc tcctttggat atttctttcg tggtggcggg
    28561 agcctttcag gagttgaaag gggaattgta gagttcacag tagaggaata taaagaacga
    28621 gcatttattt ctatgaatca acagcaggta acacaactac aaatagatga tggcaactcc
    28681 attcagggga tttctattga tagcaataaa ccttttgcga ttgtcttgcc tataaatgct
    28741 ggtaccatta ctttttatga tgtgaacgga aatactgtgg aatactggaa caatcctctt
    28801 taataaattt ccgtttatcg gagattaaaa cgaagtcata aattacgagt tattctttct
    28861 gttcatccca tgtggtatgg caagtatgag ataaaatttg aatatgtcaa cgaattattc
    28921 aagagcttat ttgaggcatc ttttcaaaat caatgatcca ttagcttata ggttagcaac
    28981 aatacataat ttgagatttt atacaaactt aatggagaaa ttaagaaaat cgtgaaagaa
    29041 ggaacgccta tgtctataga aaatattgta caaacagtga tgacatcaaa aaaatattca
    29101 aatataatcc ctgaaacgat caatagaatt tgtaatgaag aaagtataaa gtacaaaaaa
    29161 gacaaagata tattaaggtc gtcaaaaaaa agattgcata tcatttctga atcattttta
    29221 tcgatagcta atgcaaataa aattgccagt attaaaaaat gcattgataa tgatggacat
    29281 atttcaacag aaatgataga gcagttgtta ttattacata tatctacaaa agaaaggagt
    29341 agtttttata aagaattcta taatgatatt ttccgagtgg tgggaaatgt taattctata
    29401 ttggatatag cttgtgggct aaatccagta gcgtatgttc tgtccgacaa ctttgatagc
    29461 agagtaagat atgaagcgac agatataaat ttagataata ttgaattggt gaataaaata
    29521 ttatcgttgt atgatttgca agccaaaatc tatggaggcg atattttatg tgatattccc
    29581 aatggagaat gggatgttgt ttttttattt aaaatcgttc cattattaga gcaacaaaaa
    29641 aaaggatatt tccgttctat aatagaacaa ataaattcta aattttttat tattacattt
    29701 cctacaaaat cgatatccgg aaagaatgtt ggaatgaagg actattatga aaacatgatg
    29761 acaaaatata ttgaaaatag tcagatggag attatattta gaaaggcata ttttaatgaa
    29821 attatgtttg ttataaaaaa ataattgtgg agattataat gggaaaaata tatgtagtgg
    29881 caacaccgat aggaaatttg agtgatatta ctttacgagc tattgaaaca cttaaggcag
    29941 tagacatcat agcatcagaa gatacaagaa gaacattaat tttgttgaat aaatacaata
    30001 ttagcactaa attggttgca tatcataaat ttaatgaaaa agaacaagca actaaattga
    30061 ttgaattaat tttgcaaaaa gatattagta tagcgttggt atctgatgct ggaactccat
    30121 gtatatctga tccgggaagt attttaatta gtgaagcact aaaaaattca attgaagtga
    30181 ttcctgttcc gggtgtttcg tctgttatag cagctttaag tgtttctggt tttatcttta
    30241 acaaaaaata gccgttgtag gagccaatcc cacaacggct gtttttattt ccacggcctg
    30301 cgccggaagt tgatcccgga atttttaatc agcggcgatc ttttgaacag ccttttcagg
    30361 gttgttcttt cctcgtctgt gagccgcttg tattttagct ggaacgcctt gcagaagatt
    30421 tccagaaatt cctgcaccct gtcgccggtg gattgcatgg cctttcggac ttcctttatc
    30481 agttcatcgg cgggcgtggt atccggcgca ctctccatgt cgttctcatg gactttgcgt
    30541 atatcgtgaa ggatagcgtc ccatgttttg tgtgtcacat gacagaaaaa atcttcttcc
    30601 tctatctgac cggcttccag aattttcagg gaacggtctg cggcagcttc gggatgctct
    30661 tccagtatca ttgcccggac agcggccagc gcactgttga tgtcatggaa ccgcatggta
    30721 gccattccat ccacatagat ttctgcgtca gccatcagct ccgcaaattt ttcgtgggac
    30781 atcatctcgc acagcagccg ggtattgatc cgtccacttt tcagaagcgc cacggcttca
    30841 tcggtcaagt gcagttcaga cagcggggtg ttgatctgct cccggttttc tgtccggcag
    30901 aacagatagt caatggacac ctcataaaag tctgccaatg cgataaggtt gccgtgattg
    30961 atttccttat ttcggttttc ttcattttca tagctggcta aagcagattt tgaaatgccg
    31021 gtcttttccg ccagttcttc cagcttcaag cccttttcca cccgtaaatc tttcaagcgt
    31081 tcctgcaagg aaacatggtc tttcattggt ttgccactcc cttcccatgt ggttttcgag
    31141 catatataac cattatacca taccgttccg aaatcgtgga aattttcgat tttagggcgg
    31201 tattcctact ttctggacat acgggaaagg gttcaaaaat gagctatgat taagtcagtt
    31261 catcgatgga ttattccaat cgaatgaccg gcctgatggg atatgctccc cggcgatgta
    31321 gcgcaacgac ctgcggcaag gagttggagg aaccttgacc gcagcgagcg taccaacggg
    31381 aacgaaacgc tgctgtgaga ttcaggggca ggaacgacat cagggaaacc cggcagaact
    31441 gacaccaaaa cgctacccaa acatgacaaa tcggcggggc gtagtctgcc ctgtccgtga
    31501 tactatgggg cttttccaag cggctctatg gccgtatttt ccttgaaaat cgccccgaaa
    31561 catgacacca aaaaccgcta tccgcccatc tctaccgtta cggctgaaat ggacggattt
    31621 tctatgatag gaggcaccat gccgagaatg agcaagaaac ggaagttgga gctgtccttc
    31681 ttcctcaatg accgggggcg tgtggcctac aacgacctgt gccggaagtg tcagcatgag
    31741 tgcaaacaga gcttccgggc tgtcgtgatc gactgccccc attacctgtc caaacgagcc
    31801 aagcgaaagg agaaaaccga ctaaatggaa tttgaattta tgaccgcaga caccgttctg
    31861 ccgccctgta tgccacttcc taccgccatg ctacggctgc cggtcagcag caccgcaaaa
    31921 gtcatgtatg cccgcctgct ggacgctacc cttacgggag gtgcggagga taccaacggg
    31981 atactgtttg tccgtttccc cattgtggag ctggcagcgg ccttgtcccg cagctccgtg
    32041 accgtcaagc gttccttgaa agaattggag gacgccggac tgatccttcg ggtgcgccgg
    32101 ggcgtgggag aaccgaaccg catttacacg ctgctcccca agaaggaggg ctgccgtgat
    32161 gaatgaaaag ctggaaaaac tcaatcagca aattgcccaa ggcgaagcca aactgcggcg
    32221 ggcgcagcac gaagaaaaga tactggcaca tcagataaaa cagcttaccc ggaaggaacg
    32281 gacgcaccgg ctctgcacca gaggggctat gctggaaagt ttcctgataa ggccggaagt
    32341 gctgacggac gatgatgtga tggacattct caaacaggca ttttcccaaa ctggcatgaa
    32401 ggaagttgtt gcggaaagcg tgaaatgtcg ggtaaccggg gaacccctta cggagtaagg
    32461 gcgcaactat acaccggtcg agccggtgcg ttgcgccctg ccgggggctt cctgcggaaa
    32521 gcggatgatt tccagcacac ttgcgactgc ttccaatcat cacaggggac gctgcgcttc
    32581 ccctgcgctg gctgcgccag ctaccccttt gtggacttgc cgcccggaaa caccttgcgc
    32641 tgcaagctgt tcccgtccag caagttctat aattttatga cgagcaagat ggaaagtttg
    32701 cttgacattt cggatggatg tgatatacta aaaacacgga aagcaaactt tccattagaa
    32761 aggaggttcg catgaaaaat cgactggaag aactccgcaa acagcgagga atcaagcaag
    32821 aagaattggc aagcgccttg gaagtatcac gccaaacaat cggctcttta gaaaacgggc
    32881 gctacaatcc gtctattcag ttggcgttca aaattgcccg atactttcat atgacgattg
    32941 aggaaatttt tatttatgag gaggatggac aatgaaatct aaaattacaa atcatgcaac
    33001 tatcattata ggattgcttc tgctggcagt gagcttgtac ctaattaaaa caagcaatga
    33061 tccgcaggga ataatgagaa cattacccta tgtctgtatt ggcattgggt gtggcttgtt
    33121 tggacatggt atgggaaatg tgatttctga acgggccatt aatagttacc ccgaattaaa
    33181 aaggcaactg gatattgaaa aaaatgacga gcgtaatgtg gcgattgcaa accgggctaa
    33241 gggaaaagcg tttgatatga tgacctttgt ttttggggct ttaatgttat catttgccct
    33301 tatggggata gatatggttg ctgtacttct gcttgttttt gcatatcttc tcgttcatgg
    33361 ctttgctctc tactatcgct tcaagtttga taaagaaatg taactaaata gtagccgccc
    33421 accggtggcc ccaccaagca ggaaatccga aacggtttcc tgcttttctt ttgcccaaaa
    33481 tgaggtggca aaccaccacc ccatccaatc aaccacagga aggaggaaac cgttatgccc
    33541 tgtccccacc atgatataaa aatcgttcag cgcagcaacc gccagtcagc cgtagccgcc
    33601 gcagcttacc agagcggcga acggctgttt tccgagtacg accagaagca gaaatactat
    33661 gccgagaaac gagggatcgt ccacacagaa atcatgctgc cgccccacgc cccgccggag
    33721 tacgcagacc gcaacacctt gtggaacgct gccgaagccg tagaaaaaca atggaactcc
    33781 cagcttgccc ggagaatcgt gcttgccata ccgagggaaa tccccccgga gcagcacgcc
    33841 gacctgatcc gggactattg ccgggagttt ttcgtttcca aaggcatgat tgccgatttt
    33901 gccattcacg acaaggggga cgggaatccc cacgctcaca tcctgctgac catgcgggca
    33961 atggacgaaa acgggaaatg gcttcccaag agccggaaag tgtatgacct tgacgagaac
    34021 ggcgagcgta tccggctccc gtccggcaac tggaaaagcc ataaggagga caccgtggac
    34081 tggaacgacc agaagtacgg cgagatctgg cggcagggct gggcggctgt cgctaaccgc
    34141 tatctggaag ccaatgatcg cccggagcgc cttgaccttc gttcctatga gcggcagggg
    34201 cttgataaaa tccccactgt ccacatgggg ccagcggtca gccagatgga gaaacgaggg
    34261 atacagacca acattggcaa tctgaaccgg gacattaccg ccgccaaccg gctcatgcag
    34321 tccatccgtc agatggtgcg ccacctgaaa ggctggattg cagacctgaa ggagaaaaag
    34381 gccgccctgc tggaagcctt gcaggaagcg aaggaaccca ccctgccgga gctgctgggc
    34441 aaatatctgg atttgcgccg ggaggagcgc accggctgga cgagcaaagg ccagctcaaa
    34501 ggcactgtaa atgacttcaa caaggttatg gacgccatcc gctatctgcg ggacaaggaa
    34561 ctgtccaccg tggagagttt ggacgcctat ctggataccg tcagcgggca ggtcgtttcc
    34621 atccgggcag agatgaagcc gaaggaaaaa cgcatgaagg agatcgacac cctgctttcc
    34681 catatcgcca actttgaagc ccacaagccg gttcatgcgg aatatgccgc catccggttt
    34741 aagaaaccaa aggaacagtt tgccgccgcc catcgggacg aactggacgc ctacaatgcc
    34801 gctgtccgct atttcaaggt gcatttggag ggaaccaagt acagcaccaa aaagctgaat
    34861 gaggaacgaa cgcagcttgc cggagaagtg gccgagtata aggagcgcct atcagcggtg
    34921 caggaggatg tgaagattct ccgggatgta cgccactggc tcaatcaggt gcttccgtcc
    34981 gagcagtacc gccagactgc cgagccgggc aagaaaccgt ccatccagca ggcagtcaaa
    35041 ggccgggcac agcgtatcca ggaggaacag gccgagaaac gccagcagcc ccgcacagag
    35101 aaaaaacagg atatggaact ttaacaggcg cttaccattt tcttttcgag aatgacaggc
    35161 gcttttctta ttttcaggag gatttagttg aatgtatttg aagctgtcaa gcagtctgtt
    35221 accacccggc aggctgccga gcatttcgga atccgggtgg gacgaaacgg gatgtgcgtc
    35281 tgtcccttcc atgccgacaa aaacccaagc atgaaggttg accggcgctt tcactgtttc
    35341 ggctgtcagg cagacgggga tgtgatcgac tttgtttccc gtctggaagc tgtcagcccc
    35401 aaggaagccg cccttatgct ggcacaggag ttttccatcc cctatgagga caaggagccg
    35461 cccagccaca ggaaaccacc gcaggaaacc ccggaacagc ggtttcgccg catggagcgt
    35521 tactgcttcc gggtgctgtg tgattaccga aacctgctgc gccgctggaa acgggactat
    35581 gcccccaaca caccagacga ggatttccac cctttgtttg tggaatccct gcaaaagcag
    35641 gactatgtgg actatctgct ggatgtgctg ctctccccgg atatggagga acgggcagcc
    35701 tttatcatcg actgtggaaa ggaagtgagc aatcttgaaa gacgaatggc agagcttacc
    35761 gccggagata cggcaagccg cagaacagac catgcaagaa gcaccgccgt tccggagcgt
    35821 tgaggaagtc aaagccatgc tggaaagcac cgagaaaggc ggcgtccgca acagtatccg
    35881 caactgcctg accgtctttc agaatgaccc gctgctttcc ggggcaatcg caaaaaatct
    35941 gctgaccgag cgaaccgaca tcatcaagcc catcggctac caccgtaccg gcaccgccat
    36001 cacagacacg gatatgaact atctgctcct gtatctggaa gaaacatacg ggttgaccag
    36061 cgaaaagaag attgccgccg ccatcgggat cgttgccaat gaaaatggct atcaccccat
    36121 ccgggactac ctgaacggcc tgacctggga tggaaccgag cgtatccgta cctgcctgca
    36181 ccacttttta ggagccgaca gcgaccagta cacctatgaa gccctgcggc tgtttctgct
    36241 gggagccatc caccgggcgt ttcatcccgg ctgcaagttt gaggtcatgc tctgtctggt
    36301 gggcggtcag ggagccggaa aatccacctt tttccggctg ctggccgtga aagacgagtg
    36361 gttttccgat gaccttcgca agctggacga tgacaatgtg taccgcaagc tgcaaggcca
    36421 ctggattatc gagatgtcgg agatgattgc caccgcaaat gccaagagca ttgaggaaat
    36481 caagtctttt ttgagccgac agaaggaggt ctataaaatc ccatacgaaa cccacccgga
    36541 ggacaggctg cggcaatgcg tgttcggagg aacttccaac gccctggact tcctgcccct
    36601 tgaccgttcc ggcaaccggc gctttctgcc ggtcatggtg tacccggaac aggcggaagt
    36661 tcatattttg gacgatgaag ccgcttccag agcctacatg gagcagctat gggcggaagc
    36721 catgaccatt taccgcagcg gggattttaa gctgtctttc agccccgaaa tggtgtgcta
    36781 tctcaaagaa caccagcggg atttcatgcc ggaggacacc aaggccggga tgatccaagc
    36841 ctaccttgac cgctacaccg gcagcatggt gtgttccaag cagcttttcc gggaagccct
    36901 gaaccacccc tttgacgagc cgaaacaatg ggaaatccgg gaggtcaacg atattatgaa
    36961 ccattgtgtc acaggctggc actacttttc caatccccgg atgttcccgg aatacggcag
    37021 gcagaagggc tgggagcgtg aagccccggc aacggatacc agcaacggag ccgaaaaaat
    37081 cccggaggga tttgtggagg tcacagagca gatggagctt cctttctgaa aatcccaccc
    37141 cgttgccgac tttgttgcac tcccgttgcc aggctggttg ccggggaaag ccccgtaact
    37201 gcgggctttt tcccctatca gcaaccaaag caacagaaaa ataaaagaaa atataaattg
    37261 taaccaaacc gccagacaga gatggttttg aggttttttg aagcccgttg ccggacttcg
    37321 ttgccgacct tctccttgtc tggctattct attatggagg ataactgcct atggcaaaaa
    37381 cgaaaacaga gattcatgtt accacagttt ttgacggcga gctggacgca gcggatgtct
    37441 ttgtgagcct gattgcccaa aaatatgggc agaataccgg caaggataat cttgcgaaaa
    37501 cacaagattt aggatataat aaagacgagg ttcagaagaa ccgtgttccg tctggattgt
    37561 gcgggtaaac ggttatgatg aacggatatg aatacacagg catggacgca attctggcgg
    37621 gcagcttccg ggcggccatc tattgcaggc tttccaaaga cgatgacctt gacggggaga
    37681 gcgccagtat tgccaaccag cgggatatgc tggaaagcta ctgcgaaaag cagggatggg
    37741 aggttgtggc agtctatcag gacgatggct acacggggct gaacatggag cgccccgacc
    37801 tgaaacggat gttaaaggcc atcgagcgaa ggcaaataaa tcttgtgata acgaaagatt
    37861 tatcgagatt gggccgaaac tacttgcaga ccggcacact gatcgaggac ttcttccccc
    37921 gtcacggcgt ccgctatatc gccatgaatg acggtatcga caccatgcgg gacaacaacg
    37981 acattgcgcc gtttaagaac atcctgaacg agatgtacag caaggatatt tccaagaagg
    38041 tacattcctc gtatcttctg aaagcccagc aaggccagtt taccggctgt gtggctccct
    38101 ttgggtatcg gaaagacccg gaggacaaaa accacctgct ggtggacgag gaaaccgccc
    38161 ccattgtccg gcaaatcttc ctttgggcgc tggaaggaca cggccccaac tttatccggc
    38221 gcaggctgga agaacagaag gtgccttgcc ctacatggtg gaaccgggaa cggggcttcc
    38281 gcaatgtccg caccaaatgg gaaaagaaag acccggaaaa cgggcggtat atgtgggact
    38341 tctccgtgat aaaagacatc ttgatgaatc ccgtttatac cggggcgata gcttcccaaa
    38401 agaaggacta ccgcttcaaa atcggcacca ttggcgagaa gaagccgcag gactggatcg
    38461 tggtggagga acggcacgaa ccgttgattg acagcaaaag ttttgccatc gtgcaggaca
    38521 agctgaaatc ccgccagcgc ccccggcagg acggggaaac cagcctgttt gccgggctta
    38581 tcaagtgcgg cgagtgcggc aagtcgctga ccatccgcac cacccacgcc aagcacccgc
    38641 agcagattta tgcctgtaag acctatgggg cgttcggcaa gaaccactgt tcccagcacc
    38701 gggtagagta cgacaccctt taccaccttg tcctgaacaa aatccgggag tgcgccaagg
    38761 ctgccctgac cgatggggaa gccattgccg ggaagctgac caacacctgc gaagccgaac
    38821 agaaaggcca gcgggaagcg ttggagcgtt ccctgacaaa agacgaggaa cggattgagg
    38881 ttctggaaaa gatggtgctg cggctctatg aggacatggt tgccgggcgg atcagcgaag
    38941 ccaacttcaa tctcatgctg gacaagacgc agaaggaaca ggctgaactg aaagaacggg
    39001 ttgcacaggg gcgcaagaag ctggctgatg aaatgcggct ggcgatggac gccaaacaat
    39061 gggtggacgc cattcaggaa tatgccgaca tcacggagct ggacgcagcc accctaaacc
    39121 gcctgattaa agaaatcgtt gtccatgaac acatcgacag cgataagaca cgacacattt
    39181 ctatcgaaat tcacttcaat ctcaaaccca tcccggaggt ggaacaggtc aagggctgac
    39241 ctgtctacgc tggggcggtt ctaaaacaat tccatatatt ttttgacacg ccgccgcccg
    39301 ccatcgagca aggttttact cctaattagg gataaaacaa cttatggctg gaggtggcat
    39361 agtacttatc ggattaaagc tgattccact tcttgcaaat gtactcagat aagaagaaaa
    39421 ggagaaacta aatgtttggg atattcgaca aaatagagga gtttttcaaa gagtttctat
    39481 taggcggtat ccaagcaaac ttagagtcta tgtttcttga catcaacgat aaagttggtg
    39541 cgattgcaac ggatgtagga aagacaccga tggggtggaa cggacaggtc ttcagtttta
    39601 ttaaaagcat taacgattcc gttatcatcc ccatagcggg gctaatcata acggcagtcc
    39661 tttgtatcga gcttatcaat atggttatgc aaaagaacaa tatgcacgat acagatacgt
    39721 ttgatttttt taaatacatc ataaagatgt ggattgccgt atggttagta tctcatgcct
    39781 ttgagttttc aatggcagtt tttgatgtag ctcagcatat ggttaataaa gcagcaggag
    39841 ttataaacac atcagcaact gtttccggaa atcagattgt agcaatgatt gattctctaa
    39901 aagacaaagg tcttggagag cttgtgatga tactctttga aacctctctc ataaaggtag
    39961 ctatacaggt tatttccatt gtgataatgc ttgtggttta tggaagaatg tttgagattt
    40021 atatttactc atcggtttca gccattccat ttgccacaat gggaaacaaa gagtggggac
    40081 agattggaac aaactatatc aaaggattgt ttgccatagg tttacaagga ctctttttaa
    40141 tggtttgtct tggaatatac gcagtattag ttaagacaat aaagataaca gacatacaca
    40201 caagtacctt tacgatactt ggatatgcgg ttttgctagg gttaatgatg ttaaagagcg
    40261 gaacactggc caaaagcgta ttaaatgcac actaaaagaa aggaagggta atatgaacaa
    40321 aagaaaaaca gtatttatag tagtaattgg agcaggtatt atggcggtga tagataggat
    40381 cagacttcac agcaaggtaa atgacttgga agaaagaaaa aaagacatcg gtcgctgcca
    40441 caatgatttt tgcctaatgc aggaaagatt taacagaagt atagatgaac agattacaag
    40501 tattcaggaa gaaatcggct ccgtatatga gcatatcgag gagctttcaa agggaaaaga
    40561 agatgggagg taagttatgg cgtatgtacc tatccccaaa gacttaaaga agttaaaaac
    40621 aaaggtggca tttaacttga cgaaaagaca actgataggt tttacgattg caggacttat
    40681 tggaatacct atctatttat ttatgcgaaa ggtcgttcct aatgatatag cagtcatatt
    40741 tctcattgtg tccactctcc ctatattttt catcactctt tttgaaaagg acggattgac
    40801 atttgaaaaa tattttaaat atatttacct tcataagttt tatcagccac aaaaaagagt
    40861 gagaaaggag gtttaccttg aacaaaaaaa gaaagattca gcagctaaag ttagaacaaa
    40921 acaaaaagaa gttaagaagt caaagacagg acttaaagca aaataaggga aaatctaaaa
    40981 aagataaagg tgggctactt gaccctatct ttaggaaaga accaaaaaga tatactgttg
    41041 aagataccat tccctacctt agactattaa aaagtgggat atgtcagatt gatgaaaatc
    41101 attttaataa gagtatcgcc tttgaggata taaactatca gcttgcttta gaagaagata
    41161 gggatttgat ttttaatcag tttgcaaact ttcttaattc ctttgatcca agtataagca
    41221 tagagttttc atatatcaat cagcttggca gaaatgaaga aatgaaatcg gcaattcaaa
    41281 taccggataa aggagatggt tttgacgata tacgtcttga gtttcgagaa atgcttaaaa
    41341 gccagattgt aaaaggaaat aacggactga aaaaatcaaa atatgtaact tttacagtag
    41401 aagcggataa tttagaacag gcaacatcaa aactggaaag aatggagata gatatattat
    41461 caagctttaa aagtatggga gtaagagcag aaagccttaa cggggaggaa aggctaaaga
    41521 tacttcatga tgttttaaat ccgaataaga cctttgaatt ttcatataag gacttaaaga
    41581 aaaaggaaag tacaaagaca tatattgctc cagatgaatt taactttaca ccgtcaaggt
    41641 attttaagtt tggaaatttt atcggagcag caagtcattt tcaaattctt gcaagtgagc
    41701 tttcagatcg tatgctgtca gagtttttgg atatagatga taacatcaat atctcttttc
    41761 atatcaaggc aattgagcag tcagaagcca tcaagatggt caaaagaaaa aataccgata
    41821 ttgacaagat gaagattgag gaaaacaaaa aggctgtaag gtcagggtat gacatggaca
    41881 ttcttccaag tgatttaatt acctatgggg aagatgtgaa aagcctatta aaagacttac
    41941 agacaagaga tgagagaatg tttgttgtaa ccatcatttt tatgaacttt gcaaggacaa
    42001 ttcaaaagct ggataacacc atttctcaaa ttagctccat tgccaataag cataattgca
    42061 agttaaaaag gcttgaccac tcacaggagc agggcttaat cagtgtactt cctcttgggg
    42121 taaataagat tgaaattgat agagggctaa cctcatcatc tacggcggtc tttatgccgt
    42181 ttaccacaga ggagcttttc ataaactcgg caaacagttt gtactatgga ctaaatgccc
    42241 taagtcataa cctgattatg gcagatagaa agaagttaaa aaacccgaac ggtctaatcc
    42301 ttggaactcc gggaagtggt aaatccttta gtgcaaaaag agaaatggca aatgccattc
    42361 ttacaaccga tgacgatgtg attatctgtg atccggaagg cgaatacgga aaccttgtaa
    42421 gacaatttaa gggaaaagtc ataaaggtca gcagtaagtc gaaggactat ctcaatcctt
    42481 tagatataaa tatgaactat ggagatggtg acgcaccact aaaggacaaa gcaaacttca
    42541 ttatgtctat gcttgagctt gttgtaggag gtagtggtct tacagcagaa gaaaagtcgg
    42601 ttatagatag atgtctgcct aagatttatg gaaagtattt tgaaaatcca attcttgaga
    42661 atatgcctat tcttcaagac ctgtatgata tgttaaaaaa tcaggaagaa aaagtaggta
    42721 aaaagttggc aacagaaatg gagatttatg taacaggatc tcttaatgta tttaataaca
    42781 ggtcaaatgt ggacttaaat aagcagcttc tttgttttga tattaaggag ttaggctcgc
    42841 agcttaaaaa aattggaatg cttgttattc aggatcaggt gtggaacaag gtatcgcaaa
    42901 acagaggaaa taaggctaca aggtattata tagacgagtt tcacctattg ttaaaagacg
    42961 agcagacagc ttcctattcc gtagagattt ggaaaagatt tcgtaagtgg ggtggaatcc
    43021 cgacaggcat tacacaaaat gtcaaagact tacttatgag taatgaaatt gaaaatatct
    43081 ttgataatac ggactttgtt ttaatgctta atcaggcttc aggcgataga gaaattttag
    43141 caaggaaact taaaatctct cagccacagc taaaatatgt aactaattcc aatgcaggag
    43201 aaggactttt gttctttgga aacaccattg ttcctttcct tgataagttc ccaaaagaca
    43261 caatacttta tcagaagatg acaaccaagc ctgaagaagt gaggtaggct tatgggaaag
    43321 aaactgaaaa aggactttaa ggaaaggcaa agggcaagga ttgaaaagga aacgctgcaa
    43381 agtaatatgg gaattgagcc tgaagaaagt aagttaaagt ataacgatga ttacagaggg
    43441 aaaattgttc atgataaaga cagattccaa gataaggtgc atgagaaaac aagcaaaatg
    43501 acctctgaca gtgaaccagt tagagggact tccaaaagaa atgcaagata tagagcttcc
    43561 aataaggata gcataacttc ttcaactgaa actggaggtg taagtcagga agaaatcggg
    43621 aaagcggatt atggtacgga ggttaaggat ggaaaaatct atgatccttt aggaaaagac
    43681 cttgataatg acggaatcat agatagatat gacaatgatt ttagggacag cgactatttt
    43741 gagtcaacct atgatgtaga tggattaaaa aaagatgaga ttagcggaaa tccggtaaat
    43801 aagcaaaaca ctcaaaagaa aaattataag agaaaaaact attccgataa gctctataca
    43861 agaaagaagg acaaagaagc taaagaggaa aaagcagata ataaaaaaag cggaaaagaa
    43921 gccgttcttg atagagaaaa gaaaaacagg ctttctaaag aacaggaaac aattttcgag
    43981 aataagactt ctaaagtatc tgctttatca ggcttagcaa aaggaagcga aaccgtaaga
    44041 gattatttat ctcatggaag tgatgaaaat caaggggtag aggcaggaga aaagacagca
    44101 gatacaagct caaaactcat tcatggcatt aagaactact cggataagaa aagaacaaag
    44161 aaaggatatg accttacaaa taaggactat aaaatcagaa agcgaaaatc caaattagag
    44221 tttcgagatg ccaaagagga actgaaaaag acggaggagt atcaaaaagc aaataacttt
    44281 aaacgatttc aaaaaagaaa gcagatgaaa gattccatta aaaggcagaa taagtcaagg
    44341 cttagagatc gcattaagga agggcttatc ggcagtctta aaagttcaaa ggaagttata
    44401 ttaagaaaaa cgaagggact tatgattatt ttcataggtc ttattctttt gggaactttc
    44461 tttattaact ttgcagggac aggaatgaca ggctttatga actctacaag tacagttctt
    44521 acaacaagtt atttatcaaa gcctaatgtt ctcacagaaa ttcatcaaca attttctagt
    44581 atggagcaag gattacaaga tgagcttgaa agtgttgaag aaaattatcc cggttatgat
    44641 gagtatattg tcaataaaga gggagatatt ggtcataata cccatgagct tttatcctac
    44701 attacatcaa ggttcggaga agtaaagaat gtatcagaag taagctctgt attagatgag
    44761 ctttttaagt ccatgtatag gcttgagtat aaggaagaaa ttgaaatcag atacaggaca
    44821 gttacagaaa tctatactga tgaagatgga aacgaatata ccgaaagcca tgaagaacct
    44881 tatgagtaca agaaactcat tgtaactctt tataaaagag aaatggatag tgttgttcga
    44941 gaagtgtttc aaaattatcc agataatgtg aaacattatg aagcattact tgcaagtctt
    45001 ggtaatatga gtgacttttt tggaagtgga agtaatgatt attctgaaat aataaacaat
    45061 cctaattttg gcaatccggg aatagctttc aatgaagaaa cggttaaacg acttttctca
    45121 gaagcagaaa gacatatagg aaagaggtat gtttttggtg caaatgggcc gtcaaatttt
    45181 gactgctctt catttgtgtg ttggagcttt actcattcag gtattaaaaa tatgccaaga
    45241 acaacagctt ggagaattta tacggattac tgtaatcctg taagtacagg tgaagccaag
    45301 gcaggagata taatattctt taaaaatacc tataatagtg gaagcccgat ttctcatgta
    45361 ggtatatatg ccggaaatgg gatgatgata cacgcagggg atcctattca atatacttct
    45421 attaactcac catattggag agaacatttt tatgggtttg gaagaccaag ataggaggat
    45481 acaatttgaa aaaagaactg acagcagtaa aaaatagaat aaagaaattg aaagataaaa
    45541 aggctctgat tgatgaagaa ttggagcctt tgttcattcg tgaagaagaa cttgaaaacg
    45601 aagaaattat tgccatttgc agaaaaaata acattacaat ttctgatctt atggcaaaag
    45661 taaacagaga aaaatcagaa atgaaaaagg agaacggaaa tgaaaaaaat gaagaataaa
    45721 aaggctgcgg gagtaattct tggagcagtg cttttgatag tgggagtagt aatctcttta
    45781 tttgcaggta gtaagattgt atttgcacaa ggagggttgt ttacgacact tgtaaatccg
    45841 atagaagccg ttgctcaaat agaagtagaa gttaatgtta agtatatttt tgaagatgaa
    45901 aaagtcttta aggaggaaaa gattaaggct gaaaaggggt ggcttcttga cagtggagat
    45961 cttccaataa ttcctgatga tatgaaattt atagatgaat ttctatttta tgaagtaaag
    46021 ggagacggga aagatgaaat tatccgtaaa gttacaaaga ttgatgttaa ggataaggaa
    46081 acgcaaacag aaaatgaaag ctcaaaatct gagcaaagct ctcaatcaaa tgcttcaaag
    46141 atagaaaata agggaactca aacagagctt agcaaagatg atatttctaa aatggaaagg
    46201 gagtctaagg aacttaaaga aaaacttgat aagctaaatc aggagattaa ggacaaggat
    46261 aaactaagcg agaagcaaaa ggataaaatc aaagaccttg aggagaaaat agataaactg
    46321 aaggaaaaac ttgaaaaggg caaagatgat aaggacttat cagcagatat gaaaaaggag
    46381 atagacaagc taaccgataa gattaaggaa cttgagaaaa aggctaatga ggtcggtaga
    46441 gctcctatta tgcataatcc taatgtaaca ccaattagcc ctatttccgg aattaaacaa
    46501 ggaacaggaa atgtatcacc acaaacatct ataaatagca gtggtaaagg ttctgccacg
    46561 caggaaagta aaggggcaaa tacaaaagat acaaacagca atgaatcaaa ggagaaagaa
    46621 aagcaagttc gttatccaaa taagctgaca ccaaaacagc ctgctaataa tggtagtcac
    46681 gattcttctc tggatggttc aagtaagaat ataaatacca acaaaggagt tgcttcagaa
    46741 ccttcaaagg cacgaggaac agttactgaa aataaggata atgcaaacaa aaattatcct
    46801 attcaccata atgacagtaa agataataaa gaaacggata aatattctgc tgatgcaaga
    46861 cagtttgtta cctttacaac taaaaatggt aagacctttc atttaatcat taaccatgat
    46921 gaggatagcg agaatgttat gctcctaact gaagtatctg aagatgatct cttaaatatg
    46981 gtagaaaaga aagcaccgga gaaagagatt gttaaagaag aatcacaaaa agaagagcct
    47041 aaacaagtga aaaaagaaga aagcagtaat acaggaacct acttaattct cattcttgta
    47101 gtagcgggag ccttaggtgc aggatattat tttaaggtta ttaagaaaaa agaagataag
    47161 gagcttgaag gctttgagga ggaagatgat gatttctttt cagaggcaga agaaagcgaa
    47221 aatgaagtaa acgaagcagt aacagaagat aaagaagatg atgagataga gtaaacttgt
    47281 cccccaaatt atataatatt tggggcaaaa cacagggcga gagagtaaaa tctttcgccc
    47341 tcttatattg aaaacaggag gaaaactata tgaaacttgt aatagcagaa aagccaagtg
    47401 ttgcaatttc tattgctaaa gtaattggag caacaaaaaa gaaagacgga tattatgagg
    47461 gaaacggata caaggtaagt tggtgtgttg gacacttaat tcagatggca aatccggaaa
    47521 gttacgatga aaagtacgct aaatggaata tggcggattt accgattatt ccaagagaat
    47581 ataagtatga gattgcaaag tccacaaaga aacaatttac gattcttaaa aagttgatga
    47641 atgacaagga aatagacatt gtgataaatg cttgcgatgc aggacgtgaa ggagaagcaa
    47701 tcttcaggct tgtctataat caggcaaatt gcaaaaagaa gatgaaacgc ctttggattt
    47761 catcaatgga agatagtgca attaaagagg gctttgataa cctaaaagac ggaagttttt
    47821 acgataatct ctttgaatct gcacaagcaa gagcgattgc ggattggctt gtgggaatga
    47881 atttaagtag gctatactcg tgcctatata agcagaatta ttctgttggc agagtacaaa
    47941 caccaactct tgccatgatt gtaaaaagag atgatgaaat agcaaatttc aagaaagaaa
    48001 aatattttac agtagagctt gatttagacg gcttttctct ttctaccgat agaattgatg
    48061 ataagacagt tacagagcag cttatcaatc tattaggtaa ttccatagaa attaccgatg
    48121 ttattcaaaa ggaaaagata acaaaacctg agctgccatt tgaccttaca acgcttcaaa
    48181 gagagtgtaa taagtattta aattatagtg caaagcagac cttagattat acacaaagtt
    48241 tatacgagaa aaaactgatt acctatccaa gaacggacag tcgttatctt acagaagata
    48301 tgattacaag tacaatcaac aatattattg gaaagcatga ttttgataca ggtcgcatta
    48361 agatggtatt taattctaaa agggttacag accatcatgc gattataccg acagcaagca
    48421 gtatgaatga ggacttagct tcacttccgg aaagtgaatt gaaggtatat gaacttgttt
    48481 taaataagct acatgcaagt gtaggctatc ctttaattga gaatactaca aagattgtag
    48541 ctgtatttga cggatttgaa tttacaagca gtggaaaggt gattgtagat gagggcttta
    48601 ccaaatacct aaaaaaatat ataaaaaaga aaaatgaaga taccgtgttt ccggatttaa
    48661 agattggtga tagtcttgat attaaggaga aagagataaa agaaaaatac acatcaccgg
    48721 ctaaacattt tacggaagat acacttttaa aggatatgga gcttgcagga aatgacgcac
    48781 tggaaaaagg tgtagaagtt gagcgaaaag gacttggaac acctgcaaca agagcaggca
    48841 ttattgaaaa tttaatcttt aagggttttg tagaaagaga taagaaaaac cttattgcta
    48901 cacacaaagg aataagcctt gtaacgattg tctcagatac ttttaaatca gcaggtacaa
    48961 ccgcgaagtg ggaaatggag ttatcggata ttgcacaagg caaagcttca aaggaaaatt
    49021 ttttaaaagc gattgaaaac gagataaaag aattggtttc aacttatagc aagtaagata
    49081 gcactaattt taatatcgaa aaaatgtcct aaaaatgcta taatttttgt agggaaagta
    49141 ggaaatatgg aggtgtttta tgtcggaagt atttatgaat aatgcaaagg ttatggcaaa
    49201 gggacaggtt actattccaa agaaaataag agaaatatta gaaatcgaga atggagatta
    49261 tgtaactttt gttgtaactg aagggaaaat acaaattgtt aattcaaaaa cttttataga
    49321 gaaaaatata cagggcagga aataaattat agatggcaga tgcagatagg aggaatttat
    49381 gaataaagaa aagaattctt tacaaaatat aaacatagaa aaaaacaaca attcatttga
    49441 agaaattgtt atgattgtag aaaatgcaaa ggatcgtgca tatagaaaag ttaatgaaga
    49501 gctaatctta atgtatcaag aggttgggaa atatattagt gaaaaaacaa aggaagcatc
    49561 ttatggttca ggattcgtag ataatgtagc tgaatttttt tctattaact atcctgattt
    49621 aaaaggattt aatcgcagag gcctttatag aatgaagcaa ttttacgaac tgtacaaaga
    49681 tgatgaaaaa gtgtcaacgc tgttgacaca attgagttgg tctaatcatt taaaaataat
    49741 gtcgggagct aaaagtagag aagagagaga attttatatt aacttagcaa ttaaggaaaa
    49801 tttgacacat cgagagctgg taagacagat ggatagtgga tactatgaac gctgtatgct
    49861 ttcaaatgag ggaaatatgc ctgcaataca aagagcgaaa caggaaacac ataatttgtt
    49921 tatggatagc tatgtattag aatttcttga tgctcctaaa attggaaatg aaagagactt
    49981 tcagaagtca attcttgaaa atttgaagaa ctttgtttta gaaattggaa aagatttttc
    50041 ttttataggt aatgagtata gagtgcaggt tggaaatcat gactactata ttgatttatt
    50101 attttaccat agaggtttat cctgcttggt tgcttttgaa cttaaaatcg gagaatttaa
    50161 gccggaatat gttggtaaaa tgaacctata tctagaagct ttagatagag aagttaagaa
    50221 acaagcggaa aacccaagtg ttggagttat tctttgtgca tcaaaagatg atgaagtagt
    50281 tgagtttgca ctaagcagaa gcctttcgcc aacaatggta tctgaatata gattaaaatt
    50341 gatagataaa gaactattac aaagaaagtt aaaagagtat acaggaattg cagagcagga
    50401 gatagaaaga catgatgaaa aatagataaa tataactttg tgcatcaaaa aatgagtgaa
    50461 gtagtccatg tggaaacaat aataaaataa aagtgtttca atttagtaat atactatgaa
    50521 aggatatgaa aagaaatgcg tacaggcaat gaatttaacg gtgttgagga tactttatat
    50581 atacctttag ctgtcagaat atatgtatct gaaaaattcc cacatttttt ctatgatgaa
    50641 aaggctctat ctttgaaacc caagattcca acaggcggta tagataacaa tagtgcggaa
    50701 tatttttata tggctagtgt ttgtagacaa cagaccatag atatgaaaat taaaaaattc
    50761 cttgatgaaa ataaaaaggg aaatgtggta ttcttaggag caggtttaga gacttcatat
    50821 aatcgcatag gaaatcaaat tgtaaatttc tatcaagtag atcttccggg tgtaatagaa
    50881 gtacgaaaaa gagtgcttgg aaatgctaaa aatgagaaac ttatatcagg agatatgttt
    50941 gaccttaact ggattaaaga aatagatact tcattgccga cacttatttc tgtagcgggt
    51001 gtatatcagt attttgacga atataaaatt atagatatga ttcaaaaatt gaaagctcaa
    51061 ttttctaagg gagaattgat atttgacgca acaaactcta aaggtttgaa gcttgctaat
    51121 aaatatgtga agaaaacagg aaataaaaat gcacaaatgt attttagtgt tgataattct
    51181 aaagatttag cagataagac tggaacaata ctattggaag ttgatggatt ttttcatgga
    51241 gcattgaagg cttgtcaggg attaaaacta atgacgaggt tatatatgta ttttgcagat
    51301 aagttgcatc gaacattggt tatacatttg aggtttaatt aaattctatt caattagttc
    51361 ttattacttg tattcaacgt ataatatata gaatcaaaag gtggttagag aaaatctaat
    51421 tgcctttttt atttgtaaaa aagaaggagg aaatatgcga ataaatgatt ttcataacat
    51481 tttggagctt gtaaaacagg atattctgca aagtgaagca gaatatctga agctcttaaa
    51541 ggttgtcgga aacaatcaga gatatgactt tagaagtcaa cttagtatct acgataaaaa
    51601 tcctgaagcc acagcctgtg ccaagttcga ctactggaga gaacgcttta accgaacagt
    51661 aatgcgaggt caaaagggta tccctatttt agaggactac ggcacataca aaaaagttga
    51721 ctatatcttt gatataagcc agacagtttc aaagaatagg gatgtcaacg aggttaatct
    51781 ttggagattt gataaggaag ctcacaaaga tgtattaaaa gaaatgataa aaactgaagg
    51841 ctatgaagaa agcgaaagca ccttagaaaa tatcttttct ttaagcaggc tctacggaga
    51901 tgaaaaaata gataacctga tgaatgagct tagaatagct gacgaggata gaatttcctt
    51961 tactaaattt gtgagggatt cgataagcta tgcagtagct tcaagattta agttagacta
    52021 tcctatggat aaggagctgt taaaggaaaa ctttgcaatg cttgacagca tttctcttat
    52081 gagccttgga gaaacggtgt cggatattag cggaacagtt attgatacga ctattcaaaa
    52141 aagcaaagag ttagagctta aaaaagaagt tttaagaggt aaagaagcag gatataataa
    52201 aataaaagaa gaattagagg aggtagaaga aaatgtactt cgacgagatg atcaggaaag
    52261 aattagtgaa aacgagcgaa ttttccgaaa tggaaagtac aggcgagata atagagaaaa
    52321 tcaaggagaa tacactgaat cagttagagg aagagatgga ctttatgagg gaatatccga
    52381 atccagtgta cgcagtgatg aggctgaatt atctttcaaa cagcgaagag cagagccact
    52441 tcgagatgtt agtggatcta tacaaggaga agaagctgac caaacacctg atggatactc
    52501 agaaacaggt gatagagttt atgagagccg aaaagcaaaa actgatgatg tcttggcaga
    52561 tagagaacga gagccatcct cagtacaaag caatgatttc agccctcaaa gaaatgacaa
    52621 tcaaggaagt agtggaaatc taaaagagaa tacggataaa gagataaggg aagctgataa
    52681 ggcttctttt tccttgcctg aaaacagctc aggacaaatt aaacttacaa tacctcttgc
    52741 ccaaaatgat attgacagca ttcttgtaaa cggtggaaac catgagggtg gcagacttcc
    52801 tatcattgct gaattttcca aaggaaaaac agtagaaaaa ttaggagaat accttaaaga
    52861 taccatggga ggcggaaacg gtttcttcct taatggaact gaagtatctt cctggtattc
    52921 ggataaaggc attcatttag cctacggaac ttccgcaaga gaagatatga cgcaaatttt
    52981 aagctggagt gatgctgcaa gaagaatcaa tgagcttttg gaaaatggag agtttgctac
    53041 aaatgtagag cttttagaag cacaggacta tgaaagaaat agagtttcag aatcgctatg
    53101 gtatctatat cacgatttaa gcgaagaagg gaaagcacag ggctattttg actttataga
    53161 aacaggtggt ggctttccga aagaaacaag acagttatcg gaagctctta aaaatcctga
    53221 ctatctaaaa gatgttgttt ccgagtacag tcgctttttg acaggatata aagaaaacaa
    53281 agaggtacta agatttcact atcacaaagt agatagcctt taccagagat tacaggagct
    53341 tgcacttaca agaaaggaat atacctctaa ccttatcgaa cttccaaaga tacagggctt
    53401 tattacagaa gatgaagtcc ttgctacaat ttcaagagga agtggagtcg ataaaggaaa
    53461 agagcgaatt actaagtttt tcaaagaaaa tcataccttg caggaaaaag cgaatttcct
    53521 aaaaggtgaa tatgggatcg gtggaggctc tcatgctgta tcaggcgaaa gaggaagcgg
    53581 agaatggcat gatgcaaagg gactgaaatt acagaagaat cattgtaacg atgtttttct
    53641 tacatggtca aatgtagcaa agcatataga tgagctgctt tctaagaatc tttatcttga
    53701 agaaaagaac attgaaaaaa aggtagagat agaagaacaa aaagagccaa gttattactc
    53761 taaagacgat ccatataatc taattactga tgaaatgctt gaaaaagttc ctgaacttta
    53821 tgcacaagag gatgtagctt tagcagacaa acaggttcat gcagcataca tcattccatt
    53881 tagaagtaac tggacttggt atatgacgga atatgacagg gaaagtaaag atgcttttgg
    53941 acttgtttta ggaattgagc cggaatgggg atattttaac cttgatgagc taaaggaatt
    54001 aaatgcccag aggcttgttt tagaagattt tccaaagacc tttagagaac ttaaagacag
    54061 tgaacttaaa aagcagatgg atgagcagga gctttatcaa gtttttaacg gagagctaag
    54121 ttttgaggaa aataatatag aacttgaagc acctgaagat gtagaagaac gcaaacaagc
    54181 agaacctttt caggctactt tatttgacta tctaaaggaa aaagaagaag tagagcttaa
    54241 cgaaaaagag aacagccttt cagatgagtt tacagttaaa gaaggcgata ccgtctattt
    54301 taatcatgag gagtacaaag taagagatct ttctaaaaat caaatcacag gaaaaaatga
    54361 tttgtggctt gatcctgtaa ggcaaggtaa tcatcaaatc cctattgtag cttttacaga
    54421 taatgaggat ttattaagac aaataagtct tgaaagacct gaatttatca tcggtgatga
    54481 agttaagtac aagggaaaag actatgccat cacaaaattt gataatatgg gaaataacct
    54541 aaagactgta acggttaagg ataataccga atatcttgga ggtatgatta caggctccga
    54601 tgtaattcct tatcgccaag aaagtgagct taataaaatt tttgaaaatt tgacctataa
    54661 aaaagaggag ctttatgacc ttgcagtaag aatagatgag atagcttatg attttgattc
    54721 ttatgactat gcggattccg tagaagcaga atcagagaaa agacttgata atatcaacca
    54781 aatctatggt gatttacaag caggaaatgt ttcagtatat ataaatagtt tgaatgagat
    54841 gattgaagaa gatgttgaag atacggaaat catcaagcaa gtattaacac aattagaaga
    54901 aaaaaagatt gatattcagc ctaaacaaga agtattcaat ttcaagatta cagaagaaac
    54961 acttcctcaa aggttatctc caagtgagag gttaaataat aaccttgaag caatctctat
    55021 gcttaacaga gtggaaagtg gagaacgaga gcttgatatt acagctcagg aagttttagc
    55081 taaatatgtc ggctggggtg gactttctga tgtctttgat gaaagcaagg atggacagtg
    55141 gaaagatgca agagccttct taaaggaaaa tctatcgtca tcagaatatg aagctgcaag
    55201 agaatcaacc ctaacgagct tttatacacc gaaaatagtt atagatggaa tatataagac
    55261 tctttccggt atgggattta aacaaggtaa tatccttgaa ccgtcaatgg gtatcggaaa
    55321 ctttatcggt aatgtacctg atgaaatggg taagtcaaag ttttatggag tagagcttga
    55381 ctcggtaagt ggtcgcattg gaaaactact gtatccggaa agtgaagtac aggtaaaggg
    55441 acttgaagaa acgggattct ccaataactt ctttgatgta gcaatcggaa atgtcccatt
    55501 tggagagtac aaggtaaatg acagagaata caatcgcaat aactttctta ttcacgatta
    55561 tttctttgcc aagtccattg ataaggttcg taacggcggt gttatcacct ttattacatc
    55621 atctagaaca atggataaaa aggacgaaag tgtaagaagg tatcttgcag caagagctga
    55681 atttttagga gcaatcagac ttccaaatga tacctttaag ggtgttgccg gaactgaggt
    55741 aacaagcgat attatattcc taaagaaaag agatagcgta ttagagcgag atgaagcttg
    55801 gatacacctt tcagaagata aaaacgggct tgtatataac aaatattttg tcgatcatcc
    55861 tgagcaggtg cttggttcta tgcgtgaagt aagcggaagg tttggaaaaa ctctaacctg
    55921 tgaaccgata gcttatttag ggcaagagat aaatatggct tcattaaagg agcgtatcga
    55981 gattgcagga gaaagaattt caaaagatac caagtatgaa gaaatagagc ttttagatga
    56041 tgaaatcact tcaatccctg caaccgatga tgtaaagaac ttctcttata ccttgaatga
    56101 cgatgaagtc tattacagag aaaattcgct ctttattaaa aaggaagtat cagacaagaa
    56161 taaagaaaaa atcaaagatt atcttgagct gaatgttgcc ttaaaagatg tgatttacaa
    56221 gcagaaggaa gattttagcg aagaagaaat caaggcttca caggaaaaac taaatgaagt
    56281 atatgacaac ttttcaaaga agcatggctt tgtcaataat ttaagtaata ccagagcttt
    56341 gaaagaggat agcaactttc cacttgtttc ctccatagaa attcttgatg aagaagaaaa
    56401 tttcaaggca aagggagata ttttctccaa aagaactatt acaaaagcca aagttataga
    56461 ccatgtggat acttctttgg aagcccttgt tttatcggta tctgaaaaag gctatgtgga
    56521 ctttgactat atgggaagtc ttacaggaaa agacagagcg actttaattg aggagcttcg
    56581 aggggaaatc tacttaaata tcagagaaga acaaaatttt tacagaccac tttcttttaa
    56641 tcctgaagat ggagatttac cttttgcctg tgcaaatggc agtaattcct ataaatatgg
    56701 ctatgtaaca aaagatgaat acctaagtgg gaatatcagg gataagattg ccattgtaga
    56761 tagctattta tcaaagttaa gacaaacgga acgagagctt cctcatcttg gctttgttga
    56821 gaatggaaaa gaaaaagagc tgataagcta tgaaatgaat cgtttggaat atcaaaaagc
    56881 agagcttacc aaagttcttc caaaggaatt agaagcaagt gaaatcaatg tgcgtcttgg
    56941 tgcgacttgg ataccgatta aagatattga aaaattcatc tttgaaactt taaaaactcc
    57001 gggatatgca agatgggata ttaaggttaa attttcaaat attacaagtg aatggaatgt
    57061 agagggaaaa agcagggata aaggaaatga ccttgcagaa atgacctttg gtacatcaag
    57121 ggtaaatgcc tataagctga ttgaagatgc cctaaatctt aaagaaacaa aggtatttga
    57181 ccagattata aatccggacg gttctaaaac ttctgtctta aataaaaaag aaaccatgct
    57241 tgcaggacaa aagcaggagc tattaaaaga agaatttaag aactggatat ttaacgatca
    57301 ggaaagaaga aaccgtcttg taaaactgta taatgagcgt tttaactcaa tccgaaacag
    57361 agaatatgat ggaagcaatc tttcttttga gggaatgaat acagaaattg atttaagacc
    57421 tcatcaaaga aatgccatag caagaagtct ttatggagga aacacattgc ttgctcatgt
    57481 ggtaggcagt ggaaagacct ttgagatggt agcttccgca atggaaagta aaagacttgg
    57541 aatgtgcagt aagtctttgt ttgttgttcc aaatcactta acagggcaaa tcggtcgtga
    57601 atttatgcag ctatatccgt cggctaatat tatggtggct gataaaaaag actttgaacc
    57661 gaagaacaga aagagattta tcggaaagat tgccacaggg gaatatgatg cggttgttat
    57721 cgggcatacg cagtttgaaa aaatcccgat gagtaaggaa tatcaggaaa agcacatcca
    57781 agatcagatt aatgaaatcg taaactatgt agaagaatac aagcatgaca gaaatcagaa
    57841 ctttacagta aaacagcttg aaaagacaaa gaaaaaactg gaaacaaggc ttgagaaatt
    57901 aaatgatgat tttaagaaag acgatataat tacctttgag gaactgggag ttgatatgct
    57961 ctttgttgac gaggcacata attacaagaa cctttatctc tataccaaaa tgagaaatgt
    58021 tgcaggtatc gggcagtcag aagcctttaa gtcctccgat atgtttatga aatgcagata
    58081 tatggatgaa atgacaggag gaaaaggcat agtttttgcc acaggaacac ctgtaagtaa
    58141 ctcaatgaca gagctttata caatgcaaag gtatcttcag tatgagagtt taaagaaaaa
    58201 tcacttagag cattttgatt cttgggcttc cacttttgga gaaacacaaa gctcctttga
    58261 actatctcct gaaggaacag gatatagggt aaaaacgaga ttttcaaagt tctataactt
    58321 gcctgaacta atgagtatgt tcaaggaagt agcagatatt cagactgcgg atatgcttaa
    58381 ccttcctaca cctgaagcta attatgaagt tattaaaact gagccttctg aagaacaaaa
    58441 ggaaatccta aagagccttt ctgaaagagc ggatgatgtc agaaataggg ttgtagagcc
    58501 ggatgttgat aatatgctta agattaccaa tgacggtaag aaacttgcct tagatcagcg
    58561 tttaatcaat cccctacttc ctgataaccc tgatagtaaa gtaaatgtat gcgtaaaaaa
    58621 tgtatttgct atttgggata agacaaaaga aaataggtca acacagctac ttttctccga
    58681 tatgtctact ccaaaaggag atggagaatt taatatctat gatgatatta gagaaaaact
    58741 tgtagcaatg ggaataccga aagaagaaat cgcctttatc catgaagcga attctgataa
    58801 gcaaaaggat gaactctttg caaaggtaag aaaaggcgaa gttcgtatct taatgggttc
    58861 aacgcagaag atgggagccg gaacgaatgt gcaaaataaa ctgattgcac ttcatgattt
    58921 ggatgtgcct tggagaccag ctgaccttga acaaagagct ggtagaattg taagacaggg
    58981 aaacgaaaat gagaaagtaa atatttatcg ttatgttaca gagaatacct ttgatgcgta
    59041 cctttggcag acgatagaaa ataagcagaa gttcatttca cagattatga ccagtaagac
    59101 tcccgtcaga gttgcagaag atgtggacga aagctccctt aactatgcag agattaaagc
    59161 acttgccact ggtgatccaa aaatcaagga aaagatggac ttggataacg aggttacgaa
    59221 acttaaaatg cttgaagcaa actataagtc caaccgttac agattagagg ataaagtggc
    59281 gaaaaactat cctgaagaaa tcgcaagaac ggaaaaactc atagaagctg ttaagaaaga
    59341 tatagcaagt gtagaaccta aagcggaagg tgaggaaaag tttacttcca ttaccattgc
    59401 gggagaaaag attaacgata agaaattagc tggagaaaga ctgcttgaag ctatttccaa
    59461 agtgaaaatc aatgaaagca aggtgatagg caagtataga aatatggatt tagaggtaag
    59521 ctataacttc tttaccaatg aacataattt tagcctaaac ggtgctgcaa agcattcagg
    59581 agagcttgga acaagtgcgg acggtaacat tacaagactg gataatgctc ttgagaaaat
    59641 gcctgagaaa ttaaataggc ttgaagaaaa acttatcagc acaaaggaac aacttcaaaa
    59701 tgccaaagaa gaattaaaga aaccttttga gaaggcagat gagctgaaaa ctaaggtgtt
    59761 aagacttgca gaactgaata agctccttga tatgggagaa gtggaagaaa agagaaatga
    59821 taatccactt gtagaagatg taaaaatggc aatcattgat ttctgtaaca gagagtacga
    59881 agaaaatcat agctatgatg aatttaatac cttgtatcct gacttaaagc atatcggtat
    59941 cgcctatacc aatacaccgg atgaaaggca tggtattcag tatgagcttg atttagagga
    60001 gaaaacttgg acacagtata ttgatggtac gccaatcaag acagagagct tttattatga
    60061 gaataaagga gaaaatgaag ctcttaggaa tatgaaaaat gagattgagc tattctcttt
    60121 caatgatttg gtctatgtag attcagagga cttgaaagct gctcttggac ttgatattga
    60181 tgatgagggt aacttctatg atccgctttc taaagacctt gataatgatg gtattgctga
    60241 taggtacgac aatgacttta gagacagcga ttattttgag tccacttatg atgtggagga
    60301 taacttaaat acaaaagtgg aatctgtaac gaaagcagag gataagccgt ctattttaag
    60361 tcagatacga gcttatcaaa atgaaagtaa aacagaagaa aaacaaaaaa caaaagaaca
    60421 ggaaatttta agataagggg agggcaacct cccttttgcc atgaaaggag ataaaaacat
    60481 ggattacaaa acaatgataa atcaaataga agatatggta aattataacc ataaggactt
    60541 tgtaaaggcg attataagca tggaaaaagg tatcaatgat gaaagtgctt tagacaagct
    60601 ctatgacgct tatatggaca atgacagcct taatttactg catgaggaat ttgactatat
    60661 gattgaggat ttaagggaac agggacagat aaaagacctg ccttatgttc aggaagaaaa
    60721 ggataatctt atcaatattg ttggaaatat cgttggagag gttgatgtag tagaaaggga
    60781 aaacaagaat ggagaagcct ttaaggtggc gaatttctcc gtagtatcca aagacgatga
    60841 ggggaataag gtatatcata attgctccgc ttatggggga aaaagcgata ttccaaagga
    60901 ctttaagcag ggagattttg ttaagctctt tggacaaata agaacatcta ttgatgataa
    60961 cggcaaggaa cataggaata tcagaatact ttcttcaaaa ctgttaaagg aaaaggaaca
    61021 aatgaaagga caagaagaaa agaaagaatc tgtacttgga gctattaaga agtatcaggc
    61081 tgaagataag gaaaagccaa aagaaaagaa agaggcaaac caagaagctg agagataaat
    61141 ttatggtcgg tgtggtctta ggactgcacc gactcttttt ttatttgcac gagttcgatt
    61201 aaagagaata tatttatgaa aaaagtatat tttatggtat aatatactaa atatagtttt
    61261 cttgagcgga ggataagaat gagggctttt agttataaaa gattgttcaa aaaactcatt
    61321 gatttagata tgacaaataa tgaattgatg gaaaaggcaa aagtgagtaa aagtacattt
    61381 tataaaatga aaaacgggca gaatgtaact acggatgttt tgcttaggat atgtaacgct
    61441 ttggattgtg atattgagga aatcgtggag tgcataagaa ttgataaata gattttgaaa
    61501 ggaggttaag tatgtctaaa gaaaagtact acatgaactt accaaaagat ttatcaggaa
    61561 gcattgctaa aaatcgcttt agattagaat tattatgggg aatcagtaag ataatcgatg
    61621 aacataaagc gaacaatgaa tatacgataa tttttgattt taaatgtgat atagaattgc
    61681 ataaagagga tgaattggat ttttatcaaa tcaagacgaa aaaaagtggt aattacaatt
    61741 cgaataattt gtgtaagaaa ggtaagaata agaacaattc tattttaggg aaactatatg
    61801 ctttatattc accgaactac aatataaaat tagcaattgt gtgtaataaa caacttaaaa
    61861 tcaataataa agaaattgat tttcctgagc aatgttttgg agatttagat caggatgtac
    61921 ttgatgatgt taggaaaaag ttgtgtacag aattaaattt agacactgta tgtttagata
    61981 atgtgtttta tatatttgat aacatggatt tattaaatcc agaggattct attagaggga
    62041 aattagtaaa atcttttgtt gatattaaag gtgaagaacc tcaaaatccg aatgcattgt
    62101 atagactggt tgttgattct gttaaagaaa aagcttctta tgaatttgat tccggtactt
    62161 atgaagatgt tgtaaaaaac aaaggtatta caagaagtga atttgataaa atgttaaatg
    62221 cacataaaaa ggaatctaaa aatggaatca atgaaacaca ggagtatata aataacttgt
    62281 catttgctaa acgaagaaga tacaatacgg cacttggtaa tatacttgaa acacagcaaa
    62341 gtgagtcttt acgattaata aaaataaaaa tttataatta tattgtggaa catgaaaatt
    62401 cgttagacga tattgaaagt tatttaaaag aaatatcaaa attatttgat gacgattttg
    62461 atgtggaatt tactgatgat atgaaatcgg ttcaatacat aatgatttat tatatgtatg
    62521 cttcaggagg aattttataa tgaatgttat atttgacaaa atatatatat ttgatgttgt
    62581 aaaaaaaaag gcttattcaa cagaatttaa aaaaggagtt aatattatta catcgagtga
    62641 tatagacgga acagatagag gaaaatcagt tttattaaga agtttatacc atgccttggg
    62701 tgcggattct cactttgact cgaagtgggg agaaaaggat aaggtctata tcttattttt
    62761 taaggtggat gataaagatt tttcgattta tagatctcaa aaattattta aaatatttaa
    62821 tgcaaaaggt gatcttattt ttactactat tcatagaagt gaattagcag atttttttgg
    62881 aaatttattt aatttttcta tatgtttgcc acaaaaaaat acaaatcaat tagtaatagc
    62941 accacctgca tatagttttt tgctgaattt tttagatcaa gatgaataca tcggtacaaa
    63001 atttaattcg tttaaaaacc ttgctcagtt cagtaatttt aaacttaatg ttatttactc
    63061 acatttgggc atttacaata aagaatattt cgaacttgtg aaacacaaag aagaattaga
    63121 acttgaaatt aaaagtaaga aagaagaaat agaagaccta attaaaatga aagacagaac
    63181 cattttaatt ttggatggtt tttcatgtcc ggaaacaagc agtgcattag aaaatgagct
    63241 taatatagaa actcaaaaat attcatcgct aattaatgag atgaatttag taaggaataa
    63301 gcttgttgac ttgagaaatc aattagttga acagaatatc actttatctc aaatatctaa
    63361 attcgaaata aaagaagaaa aggaaataaa aactatttta aaatcaaaag tttgtcctga
    63421 atgtcattct gttttagatt caactttgga actaaggagt aagagatata atcatattga
    63481 taatgctctt agtctgaaag atgcaataaa aacagaaaat gcccatattg aagatgaaat
    63541 actcaaaaat gagggaatat attcaaactt aactttggtt ttggaggagc ataataaaaa
    63601 aatttgtaaa aacaaaaagg aaatcaaaga ctatatcaag ttcagaggac taaataaact
    63661 aatcgatgag attaataatg atttgactat taatagcaga gttatagaca gtacaaagga
    63721 ggctctaaag ccactaaaga aagaatttaa aagtgttaaa gataaaatta aatctgttga
    63781 tgaaaattac tattatttga ttgataaatt aaaaataaga ttcaatttaa atgagttaga
    63841 agctgacagt tatgaacaac tatctaaaaa tttttgtgca agtggaagca acaaaccatt
    63901 atcaaccgtt atatggtatc taacactcaa tgatttaaaa cagaaattta atcctgaagg
    63961 aacaacattt ccaatggttt ttgatagccc taacaatgcg gagacagatc aagagaagaa
    64021 gcaggcatta gtcgagtata ttatggaatc atcagatcaa tttagccagt taattgtttc
    64081 ggcaatagga ttttcagaaa aagattatag cattgattcg aatataaata taaaagtttt
    64141 agaaaatgaa aaatatagct tactaaatag tgagatgtat gttcagaatt atgagctttt
    64201 acaatgtatg aacgatgctt aatatagcag gggttaataa ggagatggct atctctccat
    64261 ctccttatcc atataaattc catagttctt tcgtatttga taaagctcgt ccatagtaga
    64321 ttttgtagaa gaatactctt gcattagggt attctttttt tcttgcaatt tatcaagttt
    64381 atctaaaatg ccctttgaat caggaagctt tgaataggac tttttaagtt ttgcaagagc
    64441 cgtttcatag cgtgtgattt cagccttatg ctcctcaaaa aatgccttat cggatggatt
    64501 tgctttatat tccatgtagt attgcttgta ttttttaacg gtatgaactt tttccatagt
    64561 ggtagaaagc tcctgcattt ctttatcaat aatctttatt ttatcctgta aattttgtct
    64621 ttcgtcggct actttttgga tgtactcatc aagctgttta acggatttaa tgccatgttc
    64681 tctgatataa attacagact cagccattgt attaaggtta tgtttggttg cccaatattc
    64741 gtatcctttg ctttccttta ctttcacatt ggtattcatg tcaataacat tgccgattcg
    64801 ttttttgaca ggtggggttt taatcgtttg attttctgta atacgctctt ttaatctttc
    64861 ttctgtatag tcctcaccaa tcgtcttagc tcttgtaaat ctctgtttgt cttttggctt
    64921 aaaggctata tgttttccat actttatttc ataaccaagc tccgccattt tctttagaaa
    64981 ttcttcccaa tccttagact gttttatcag acgatcaatg tcaaattgaa gtttgctttt
    65041 ccaagaagta cctttcttag attgctcatt ttcataccat gacttaccat ttgttttgta
    65101 ctttctctta tagctctcgt agaacttatc aatcacagaa aggttatttt ctttgcatag
    65161 cttatcgctt tgatacctga tttggtggta gcttttcttg ttggaatgat agcattttcc
    65221 tgttaccata tttacattat tgaagataat atggttgtgg atatgccctt tatctatgtg
    65281 ggtagataag acaaattcgt attcgtcctt taatatcttt ttacatagct ccataccgat
    65341 ttgatgtgcc atttcaggac ttgcttctcc gggtaaaaag gattgaataa ggtgtcttgc
    65401 aagaacggtt ccgtttgttc cgacatcatt tcgtgttctt aaaaattggg tatgagcagt
    65461 ttcctgatgg catttatgag tgcttactaa gatttgctca tctgtttttt ctccattaac
    65521 gatatatgaa atggcgagat taagggttga ttttatagga tgtatttttg taatagccat
    65581 aagatttaat cacctccgga agttctgtta aggagcaggg aatgaatttg ccatagctct
    65641 tttgagaaat gttcaatctc attttttata tcatttatgt catctttata gataacacca
    65701 gtggaattta cacgctttgc aatctgattg atgttgtttg ttgcattgga aagtaagcct
    65761 tgtaaatctc ggaaaggctc taaatctacc tgataaattt ccttttccaa aacgcatttg
    65821 cggataaagt ggctcatatt tctgcacctt gcgagctttc gtttctcctc aaaaagagcc
    65881 ttttcttctt cagttagttt aatttccagt ctttcatttc gtattctatt tgccataggt
    65941 aaatcctcct ttgaatgtat ttcggggtct tagggtatac cctaacaagt taaaaattga
    66001 taaaaaaaga agcatatccc agagggctgc ttcatcaatt ttttcgtaag tgtccgtaca
    66061 cttactgtgc ttgctcatat actaatcgtg taagaacaac tttaagcata atacttatat
    66121 tattatacca tagtttggaa aaataaaaaa ttatatttca tcaacgtttc ataaaaggtg
    66181 tgtagaatgt tattgaaagt agctataaaa tgctattgtg taaaaaataa aggaggaata
    66241 atttatgaaa aaaatagtat caagaggagc atgcctatta cttgcattat gtttgagctt
    66301 aaacgcagtt gggtgtcaaa aaagtaatga aaacaataaa attaaagaag gacaatccat
    66361 aagttctgaa gaggcaggca tgtcaattaa tgcaaatggc aaaaatgaaa cttttaagcc
    66421 atcagattat acattggagg caaagaaaga atatgtttat gaatatttag ggcttaaatt
    66481 taaactctca gacaaattta gaaattatat agctgataaa aagatagcga tgttagatga
    66541 tcaaagtcct atagataaag aactaaagta tgctattttg acatttgaaa aaatgacaga
    66601 agaacaaaag aatgcagtta tagaaaagat gggcgatgga tataaaaatt ggcaaaatga
    66661 gcttgaaaga attgggacta ttggtatatt cgaaaaaaac acatcagaag aaaaaatatc
    66721 taaaattaca aaatgtgata ctcatactaa aataggagtt tcaagtgatg gaaaatacga
    66781 ttgctattta agcacaaata gcggagctga aagtaatctt ctggatgaat taaaaagaac
    66841 tgagattcaa attatagata aaaaagaacg tcctaaaaat ggttttgtgc tatcagaaaa
    66901 aactgattta gaaaatacag aagcatttaa caaagaatcg gttaaagatt tacgtaaact
    66961 atcaacaaag gacataaatg gcaaggattt tacaagtaaa gattttgaga agtatgatct
    67021 tactatggta aatgtatttg caacttggtg tacagcttgt gtaaaggaaa ttcctgattt
    67081 ggttgaggtt cagaatgaaa tgaaaagcaa gggtgtaaat atcgttggag ttgttacaga
    67141 tactgttgat gataacggtg aaaacaagga agcaattgaa aagtcaaagt taatacatga
    67201 aaagacaaag gcttcatatc ctttcttaat gccagataag acaaacttta atggtagatt
    67261 gaatggtata caggctatgc cagagacttt ctttgttgat agtaatggaa atattgtagg
    67321 tgatacttat tctggggcaa agagtgcaaa ggaatggaag caggtaattg aaaaagaatt
    67381 ggaaaaaata aaaaataaat aaaaaataaa taaaagtaaa gcggtgattg gtatatggtt
    67441 aataagtttt taaaaagccg tgccttgtct ttattaatta tctttattag tatggcgatg
    67501 atttactatg gtcatagtaa tggagaaact aaagttgtct tggataaggc gataagaatt
    67561 tgtatggagt gtattggaat tggatagaat taaaaatatt ataaatggta gtacaaaaaa
    67621 agataaatat aaaataagag atcgttttag gcatttactt caatccgttt ggtttttaat
    67681 tactaattct tactttgaag ggtttaagac tggtaaaatc tacggtggga atttaaagaa
    67741 gatttgtgtt ccaggtatga attgttattc atgcccggga gcaaaaggtt cttgtccaat
    67801 aggatcacta caggcagtaa ttggaaattc aaaatataag attagttatt atatagttgg
    67861 gattttgttt tttataggag cattggtggg aagatttgtt tgtggatggc tgtgtccatt
    67921 tggattgatt caagatttgc tacacaagat tccctttttt aaaaaagttg aaacatttaa
    67981 gtttgataaa catttgagaa aattgaaata cataatatta cttgtatttg taataatatt
    68041 acctttattt ttggttgata ttttaggtca gggttctccg tatttttgta aacttatttg
    68101 tccgattggt atgctggaag gaggattacc acttgtttta ttaaataaaa gtatgagaag
    68161 tgcgataggt ttcttgtatt attggaaagg aatcattcta atagttactt tgttactttc
    68221 tataattatg tatagacctt tttgtaaata tatatgtcca cttggagcta tttactcaat
    68281 ttttaaccct atttcaatat ttagatatag attggataag gataagtgta ttaattgtgg
    68341 aaaatgtaaa aaagtttgcc aaatgaatat agatccagtt gagaattgca atcatctgga
    68401 gtgtatcaga tgtggacgat gtaaaaatgc ttgtccagtg gatgcaatta gttatggagt
    68461 aagaaaatag agaggagtag ttaggaaagt gataaaaaat gctatagcat atattacaag
    68521 aaagagaaat agaactttaa taatttttat catattaact atagttcttt cttgcttgta
    68581 ttcttgttta acaataatga aatcaagcaa tgaaatagaa aaggctttat atgaaagttc
    68641 taattcttca atatcaataa caaaaaaaga tggtaaatat tttaatgtta atcaatttaa
    68701 agatattgaa aaattaaaag aaattgaaga aaaaataatt caatatgatg gattagcaaa
    68761 actaaaagat gctaaagtag ttagcggaga acaaagaata aatagagaag atttatctga
    68821 cgaatttaag aatgttgttt cacttgaagc tacaaataat actaaaagaa atattttatt
    68881 tagtagtgga gtatttacaa ttaaagaagg taaaaatata ggagaaaatg ataagaattc
    68941 aattattgtt catgaagaat ttgctaaaca aaacaatcta aaattaggtg atgaagttga
    69001 tcttgaatta ctagatattg aaaaaagtgg aaaaataaaa agtcataaat ttaaaattat
    69061 aggaatcttt tctggtaaaa aacaggaaat atatacagga ttatcttctg attttagtga
    69121 aaatatcgtg tttgtagatt attcaacaag ccaagagata ttaaataaat cagaaaataa
    69181 taaaatcgca aataaaattt taatgtattc tggtagtgca gaatctacag acttagcctt
    69241 aaacaaattg aaagagctta aaattgatga gtcaaagtat tttgttgaaa aagattctaa
    69301 tgcgtttgaa gagtctttag agtcagtgag tggaataaaa catataataa aaataatgac
    69361 ttattctatt atgttaggtg ggatggttgt tctttcatta atcttgattc tatggttaag
    69421 agaaagaatt tatgaaatag gtatattttt atctattgga acatctaaga tacaaattat
    69481 aatgcaattt atattcgagt taatattcat atcgatacca agtataatat cttccttatt
    69541 tttaggaaat gtattactaa aagtaattgt agatggattc attaactcag aggactcaat
    69601 gatttccggt ggaagtttaa taaataatag tagttttatg ttaaatataa caacacttgg
    69661 acaaagttat ttaatattaa taagtattat tgttttatca gttgtatttg cttcttcatt
    69721 aatattaatc aagaagccta aagaaatatt atcaaaaata agttaggaga aaataatgga
    69781 tatattagaa ataaagaatg tagcatatag ttatgcaaat tctaaagaaa aagttttgtc
    69841 aggagtaaat caaaaatttg aacttgggaa gttttatgcg atagtaggaa agtcaggaac
    69901 aggaaaatct acacttcttt ccttacttgc tggacttgat aaacctcaaa caggaaaaat
    69961 attgtttaag aatgaagata tacaaaagaa aggatatagt aatcatagaa aaaataatat
    70021 atctttagta tttcaaaact ataatttaat agattattta tcaccaattg aaaatattag
    70081 attagtaaat aaatcagcag atgaaagtat cttgtttgaa ctaggtttag ataaaaaaca
    70141 aataaaaaga aatgttatga aattatctgg tggacagcaa caaagggtag ctattgctag
    70201 agcattggta tcagatgctc caataatact agctgatgag cctactggta atctagacag
    70261 tgttactgct ggagaaataa ttaatatatt aaagacatta gctaaagata gaaataaatg
    70321 tgtaatagtt gtaacacata gtaaggaagt agcagattct gcggatatca ttttagaact
    70381 aagtggtaaa aagttgaaaa aagtaaataa aatgaatttg gaggttgaat aatgataaaa
    70441 aatgcatttg cttatgtaac tagaaaaagc ttaaaatcat taattattat attagttatt
    70501 ttatctatgt caactttaag tctcattagt ttatctatta aagatgctac ggacagagct
    70561 tcaaaagaaa catttgataa tataacaaat agtttttcta tggagataaa tagacaagta
    70621 aatccaggaa cacctagagg tgggggaaat gtaaaaggtg aagatattaa aaagatatct
    70681 caaacagata gtatagactc ctatgtaaaa agaataaaca gtgttgcaga tttagttgat
    70741 tatgatatta ttgaaactca agagactctt gcgaatcaat cacctgaaag agcgaagaat
    70801 tttaaaagaa cagttatgtt aactggggtt aacgactcat caaaagaaac aaaatttgta
    70861 tcagaagcat ataaattagt agaaggaaag catttagaaa atgaagataa aaataaaatc
    70921 ttaatgcata aagatttagc tgaaaaaaat aatcttaaag ttggagataa gataaaaata
    70981 aaatctaatt tatttgatgc tgataatgag aaaggtgcag atgaaacagt agaagtagaa
    71041 attaaaggtc tattcgatgg gcataatagc ggtggagtaa gtgcagcaca agaactatac
    71101 gaaaatacat taatcactga tgttcataca gctgctaaag tttatggtaa tacagaagat
    71161 acagctgtat accaagatgc aacattcttt gtaaaaggtg ataagaatct tgatagtgta
    71221 ataaaagatc ttgggaaatt agatataaat tggagagaat ataatttgat taaaagttca
    71281 tcaaattacc ctgcattaca acaatctata tcaggtatct actcaatttc aaataaatta
    71341 tttgttggct cattaatatt tgcaggagtt gtagtttcat tattattatt cttatggatg
    71401 aatgctagaa agaaagaaat agcagtatta ctatcattag gtatatcaaa attagaaatt
    71461 tttggtcaat tcctaattga gatggtattt atatcaatcc ccgcattcgt tggttcttat
    71521 ttcttagctc aatatacagc tgataagctt gggaataata tattgaataa ggttactggt
    71581 gatatagcta aacaaatagc tagacaatct gcatctagcc aattaggtgg tggggcagaa
    71641 gctgaaggat ttaacaagac attatcaagt ttagatataa atgtattacc taaattcata
    71701 atttatgtag ttatatttat gagtttagta cttcttgtat cattgattat ttcttcattc
    71761 aatatattaa gaaggaatcc aaaagaatta ttaattgaca ataattaaaa ttttcggtgc
    71821 tttttagcac caaaaatttt ttggctttta aatatcaaat aatatgctgt ataatataag
    71881 tgtataggct taagtggagg tatatatgaa aatattaact gttgaagatg ataatatgat
    71941 aagagaggga ataagtgaat atctttcaga atttggatat actgttattc aagctaaaga
    72001 tggaagagaa gccttgtcaa aatttaatag cgatataaat ttggttatct tagacattca
    72061 gatacctttt ataaatggtt tagaagtgtt gaaagaaatt agaaagaaaa gcaatttacc
    72121 aattctaatc ttgactgcat ttagtgatga agaatataaa attgatgcat ttactaattt
    72181 agtagacgga tatgtagaaa agccattttc attgccggtc ttaaaggcta gaatagattc
    72241 tttaattaag aagaattatg gacatttaga gaagtttgaa tataaggatc tatcggttaa
    72301 ttttaatagc tatacagcta aaataaatgg tgaagaaata gatgtaaatg caaaagaact
    72361 agaagtatta aaatgtttat tggataataa tggacaagtg ttaacaagaa tgcaaattat
    72421 tgattatgta tggaaagata gcgaagaaac tccttatgat cgagtaatag atgtatatat
    72481 aaaagaactt agaaaaaaat tacaattaga ctgtataact actataagaa atgtaggata
    72541 taaattggag agaaaatgaa aaaattaaag atatttccaa aaatgttcat acaaatattt
    72601 tctgttcttg gaataataat tatattagtt cattcattag ttttttttat ctttcctaaa
    72661 acatatttgg agacgagaaa agaaaagatt cataatatgg caaatgaaat ttctagtaat
    72721 atgaatggaa aagaaataaa atatatagaa caaactttgg agctttattc taagagtagt
    72781 gaaataaaag catttataaa agaaaaaaat aataagaatg aaatacaaat caaagataat
    72841 ataaatgtta atttagaaag tgatagtaat tctttgataa ttgaagaaag agaaataaag
    72901 cttaatgatg gtaaaaaaac aaatctacaa tttgtttcta cagctgatat gcaaaaagat
    72961 gcgaaagatt taagccttaa atttttacca tattctttat taatatcaat tttattttct
    73021 gctattattt ctttaattta tgcaaaatca ataaaaaata atatacaaga aataaaaatt
    73081 gttactgata aaatgatgaa acttgataaa aaaacgcgtt taaaagttag ctctaatgat
    73141 gaagttggag aattaaaaca acaaattaat gatttgtatt ctactttatt aagaacaatt
    73201 gacgatttag aatttaaaaa taaagaaatt ttaaaattag aaaagttaaa atatgacttt
    73261 tttaaaggtg catcccatga acttaaaact cctcttgcta gtcttaaaat aatactggaa
    73321 aacatgaaat acaatattgg aaaatataaa gaaagagata tttatattaa tgaatgtatt
    73381 gaaatcgttg atagtttaac aaaaaatatt tctcaaatat tatcagttca ttctatagaa
    73441 aatttgaata atgatgaaga atatttgaaa ataaatgata cattagaaga catattaaaa
    73501 aagtatgaaa tattagcaaa tcaaaagaaa ataactgtca ataattatat attagatgag
    73561 aatgtttata taggaaaaac agctttaaag attattctat ctaatttgat tagtaatgcg
    73621 gtaaaatata ctgatgtaaa tggggtaatt aatattggga tagttaatga ttggttatat
    73681 atagagaatt catacggtaa caatagaatt tcgaatatgg ataaaatatt tgatgttaaa
    73741 tttgatttaa ataaggaaaa tagtaatgga ttaggtttat atattgtaag taatattctg
    73801 aataattaca atatagaata taaagcattg caggatgaag aattttttat ctttaaaatt
    73861 aaaatatttt cttaaacaag atataatttt gcaatagtta ttgaaaaata gagacttatt
    73921 attagccatg gaagtaaagg cttttttctt attgaaagtg aacagaaaat aattgataca
    73981 agaaatcagg atttgaacac gctaataaaa atattgttag taaaactata tgagttagtc
    74041 ttttcggatg cagagattgt ttaaatattt gatagaatag aaacttaaaa agtttaataa
    74101 aactcaatta tataagtagt agatgaatat gatggttatt tagattattg ataaaatgga
    74161 ggaaataaat atggaagcat ctaaacgttt aatgttagaa atatttagaa ttcaatatat
    74221 tcctcttttt tataatatgt atagtagatg attgtttcag gtgcgaattg aaacaatcaa
    74281 gttcatataa agaaatattt ttattctttc atttcagagg acttcttatg ccaagatgca
    74341 gaagtcctcc ttttttatct gaaaaacaag cagataaaaa agagatggag gaaactttat
    74401 ggcaaaagag tattatcttt atatcagagg acaaaaggta aaagtcagtg aagatatata
    74461 tatagtctac tggcgagaaa aagaacacga gaagtattta gagcaggtgg acaggaaaaa
    74521 ccacttgctc tttttttcag cattggatca tgacggacac tttacagata atattgttga
    74581 tgaaagtgtt gatgtagaaa agattgttga aacacagatg atgattgaag cagtaagaag
    74641 tgctataaca aggctcaatg cagaagaaag agatattatt gaacgcttgt atttcaatga
    74701 tgaaactgtt cgttcagtag caaagctcaa aagtattaca catccggctt taatcaaaag
    74761 aagaaacaaa attcttgaaa agctgaaaaa atttatcgaa gaactttaaa acttcggtaa
    74821 ccaaagggga caaattttcc ttatgtaaag tgaaggggag tttgatctcc tttactttct
    74881 tgagtaggag atacgcctgc ttatggcaaa taggaaaatg taaagaaaac agtccctttc
    74941 aaatattgct ctttgacaac tgaatagacc aaggtaggac tttatctact tactgagagt
    75001 tatgacctac gccaagactt ttctgctgat gaaaatcggt tcaaatgaat accggcataa
    75061 tgccgaggtt agaagaaaat gagcgataga taggaataca taaaagataa gcgtggcagc
    75121 ttatttcgat gatgtaagtg gtgataatga tacttcaata gtttaagctg cctcgtctga
    75181 aacaaggggc ggaggtgaga ttcctatgta gcagccaatg cacctaccaa tgtcataaaa
    75241 cttagtaaga aaaataatat ttggttataa agaatttaaa gtggaggagg tgtattagtg
    75301 gataatgact ggaatggatt ggctgatttg atagcaaatc tgattacaaa atatgctgga
    75361 gttttagatt tagagaatct tccaaatcct actccagtaa aaaatacaga ggataagaat
    75421 aaatttgata tagctaaaag cacggattga gaaagagaaa taaaagtgct ataatatact
    75481 tgagattaaa gtccaaactt ttattggtaa aaatatataa atatataata aggatattga
    75541 ggttaatgct atgtcaaaag aaaaaataaa agtatatctc tatacaagag tatctacatc
    75601 catacagata gatggttatt ctttagaggc acaaaaatca agaatgaagg cttttgctct
    75661 ctataatgat tatgagattg ttggtgaata cgaagatgca ggtaagtctg gaaaatctat
    75721 agaaggtaga gttcagttta ctcgtatgat ggaagatata aaatccggaa aggatggagt
    75781 gtctttcgtt cttgtattta aactatcgag atttgcaaga aatgctgctg atgtactttc
    75841 gactttacaa ataatgcagg attttggagt aaatcttatt tgcgttgaag atggaattga
    75901 ttcatctaaa gatgcgggta aattgatgat ttctgtttta tcggcagtgg ctgaaataga
    75961 aagggaaaac attcgtgtcc aaacaatgga aggtcgcatt caaaaggcaa aagagggtaa
    76021 atggaatggt ggatttgctc cgtatggtta tcaacttatt gatgggaaat tgataataaa
    76081 tgaggaagaa gcaattgcaa taagaactat ttttgatcaa tatgtaaata caagtattgg
    76141 agctaatggt ctttctaaat atttagaaaa tcatggtata aggaaaatac caagacaaaa
    76201 tgggaaaaat ccattgtttg acgcaggtct tataagaaaa atattaaaga atcctgtgta
    76261 taacgggaaa atagcatttg gaagaagaac tttagaaaaa gttcatggta caaggaatga
    76321 atacagacaa gtagagcagg acgattattt agtagcagaa ggcattcatc aagcaataat
    76381 cccagatgaa ttgtggcagg ctgcacaagt taaactgaaa gcacaagcta aaaagtatga
    76441 acatgtaaat aagggcaaga acatgaggac acacttatta tcaggaatag taaagtgtcc
    76501 gatatgtgga gctggaatgt ttgggaataa atctataaaa tataaaaaag atgggacgaa
    76561 gtataaagac ttctattact atggttgtaa gcatagactt atgaatagag gacataaatg
    76621 tacctataat aaacaaatta gagaagaatt gttagatgat gccgttgctg aggtaataat
    76681 caagttagta agcaacccta aatttgcatc tatgatacaa gaaaaaatta acatgaaggt
    76741 tgatacatct gctattgaaa atgagataga taattaccaa aatgagctaa gaaaaagtca
    76801 ttctacaaag tataagctta ttgaggaaat agataattta gatgtagagg ataaacatta
    76861 taagagaaga aaaaatgatt tagatgatag gctttatcgt atgtacgata aaattgagga
    76921 attagaaagt cagttaatag aagctaaagc aaaaaaagaa acgatagagg ttgagaaact
    76981 tactggtgat aacatatata aagtattgat ttattttgac aagctttata aagtcatgaa
    77041 cgatgttgaa cgcaggcagt taatagaagc actcatttca gaaattcaaa tttatgaaga
    77101 aaagcagcct aatggacaat ggctaaaatc cattactttt aagctaccta tcattgatga
    77161 agatcttaat ataagtttgg aaaatgatga gcaagttgag acgtgcgtac ttttgtccca
    77221 caaaaattcg taaacatctc caccgtcctt atacaccgga aaattgaata aaatggactt
    77281 cagaggtagc tctgattcat cacatggaaa aatctctatt tctttgatta acgaagaaat
    77341 tagggatttt ttctcttcat agctgatttt atcatacacc ttatcgaaat ttgctaatag
    77401 ggtgtagatg ttctccaagg taatggcatc ctgttccacg gcttttcggc gtagctttac
    77461 gtcctctctc tgttaagcgt agaggtatca atctgttttc ctattttgga ttttatttca
    77521 gctgaaaaat cctcattttt aatcagatct cggattgctt caacgacaag tggctcaata
    77581 tcagtttttt tgagcattgc tttgtagtca cagcttttcc ctcttgttgt ccgagccttg
    77641 ctgcacacat aatagtaaat ctctttatat gtaccgtctt tgtttgtcca tgcgtgtttg
    77701 ttggtgtaca tcggtccgcc acatttaggg cattttaaaa taccgcttaa caaatgtgct
    77761 ctgtcacgac caattttaga gggagctttc acacctgtta tttctcgttt ttcatgtgcc
    77821 tgtgtccaca gctcctcgct gataatacca tcgtgctgac agtctgcaag gatatagtca
    77881 tcttggtgta cttggcggta ttcattctta gtgtctttga ccttttctct tgttcgcctt
    77941 ccaaaagcaa tttttcccat gtacacaggg ttgtcaatta tcattctgac aaaatgggta
    78001 ctccattggg ttagtgtacc gttatcccgt ttgacttttt gaataccctg taaattaaga
    78061 tagttggcga ttttataaaa tcccatattc ccattcacat atttatcaaa gataatgcgt
    78121 accgcttcag cttcatcttc ttggataaaa agctgcttat ctttgagaaa gtagccatag
    78181 ggtgcaaaac caccattcca accaccttga cgagcttttt cttttcgtcc gttcatagtc
    78241 tgttcaatga tgttttcacg ctctatttca gcaacagcag acagcacaga gattaaaagc
    78301 tttccgcttg tctatgatga gtcaattcct tcttcaatgc aaatcaaatt cacaccaaaa
    78361 gattgaatat gctccaaaga attaagaatg tcggcggcat ttcgtccaaa gcgagagaat
    78421 ttataaacca aaacataatc aatgcctaaa ccattttcaa tatcactgag catttgcata
    78481 aaggcagttc tgccctcaat ggatttgccg gacttgcctg catcttcata aatatttacg
    78541 atttccattt cttctctatc tgcaaatcgc tttaagctgt ttttctgtcc gtccaagctg
    78601 aagccgtcaa cctgcatttc ggtacttaca cgagggtaaa gtacacattt ttttccactc
    78661 ctattcatag tcattcctct aatatagtat tggaaaacat caacacgcaa atgattttag
    78721 ttagcacggt ttatttcgcc taaaacttcc gcaccatgct tttcgataat ttgagaaaga
    78781 acatttataa aatcctgata ggcttttgtt ttctcaaatg tatgattagg tgcaggggct
    78841 ttaatcgcat caccctgtat gtttttgata ttttccattc gcaatcacct ccaagtgaat
    78901 tgcttacata aagtataagc aattttgtta tttatgaaaa gtttgtcact ttattagaaa
    78961 cagcataata aatgcaccgc aaatttacat tcaataaaaa tgcggtgcga ttaaattcac
    79021 ctgtttgccg tatttgccac gcaccccgac aaaggggata ttttgagttg ctgttggcac
    79081 agctatcata gtcccacagc accgtggctt gttttttcag aagccacgga taccgcagga
    79141 actccccttg tttctatcgt tttgggggcg gaacagcacg aagtatcatt atcccttatg
    79201 tgggtcattg cggacgagtg tgctaaactc ggtctaacag tccattttat cgctcaggcg
    79261 gtattatatt cacctcccca taccgctgtc atggcgatgt ctgttaagtc gctttgcctt
    79321 tcagttcaca taagtaatgt actcaaaata tcgtatatga aattgtcaaa gagcaattat
    79381 gctgttaggc atatcagaga aaaaatttcc tctcatatac ctttagaaaa agaggggcaa
    79441 acgacgtggg cagcttacaa attatttttc aaatactttt ctaaactgca tagccctctg
    79501 cgaattgcat ctttcacgct acttgctgat gtaccctcgg ctttggcaat agcggtctta
    79561 ctcatgccaa agaaataatg agcgtaaatg cgttttgcct gcttgtccgg caatgtggca
    79621 atggcagtgt ggagctgctc ggttgtgagc ttgcgttcgt aaatttcatc gggagagagg
    79681 gaaacaaaca aggcactatg ttcgatgccg tcaccacggt caagagagta gtaggctttg
    79741 tggtagcgta tgcggctttg attgttattc aaaaccgccg ctttgctact tatattttgt
    79801 tttggcatat gaatacctgc cgccacaaca acggtttttt ttatttccgt tgggacggta
    79861 catttagatg ttaaaattat tattttttaa ccaaagcata tattcgactg tcataaatat
    79921 tgccgtcttt gtaaatagaa tttttaagtg ttccttcaaa tacaaagcca catttttcaa
    79981 gcactttgca agaagcttta ttgtgtgaaa aaacaacagc ataaagtcta acaatatcat
    80041 attgagaaaa gcatagttct atcatttctt ttatagcatg tgtcataatt ccttgccccc
    80101 aatatgattc acctatccag taaccaattt cggcactttt ttcataaata tcttcaccaa
    80161 tagaaacacc tacacttcca atcgctttgt cgtcttcaat gattgcaaga tttatatgtt
    80221 tattattaca tctttggcag tgtgtgataa atttctcagc atccacaacg gaatatggat
    80281 atggaaaaat atttctcaaa ttttttgata cattcctatt gttagcattt tttactaagt
    80341 catttatata ttcaattgac caattttcta atttcatttt attcctccaa agtcaataat
    80401 ctatcaatac ctatcccact tatcagacaa cgatttttct tatttcctgt catttcacta
    80461 atccagttta caaaaccgcc atcagcaatt tcaattacat catcattgca atgtagataa
    80521 atcttaaaat ttattccttt gtagtaagca ttatcttcac tatttaaatc aatagtaatt
    80581 tgaatctctg gtaaagaaga ctttactata gcagtcattt tttcaaagaa tccatctcgg
    80641 tcgggataac ctccacgttt ccgtaagcaa atacttattt ttgagttttc aatatttgta
    80701 aagaggtctt tgtaatagtt tagatgcttt attagcaatt cagattcaca tgaatatgaa
    80761 cccttatctt ttccagagga aactatgcaa aatattccaa agtgagcata aaacccttta
    80821 ccttcaaagg cttgagcacg tactactctt gtagttgtag catgatggat agcatcttcg
    80881 ttacgtaata ttttcttttt taaaccgtca gcaataataa ttgctatcat attagaaggg
    80941 tccgataaag tttcggttcc ccgtaatgca ctaattacat tgttttgatt tacacatcca
    81001 aaaacactac aactaccaag tggagcggag ggagaaagta gcagattttt aattccaagc
    81061 ttttctgaaa gggataacaa cttcacctct aaattatgaa aggcgattgg gtcaaaatgg
    81121 cttggactag tgaaacgatt ggtagaaaac gcagctaata tgttttgagg tgtctttttt
    81181 attacagctg actgaaagac acttaataga aaagagttta tatccgagct atttagattt
    81241 actatcttat ctactatttt tacatcacct aagatgttta aaattctggt tatagcaaca
    81301 ttcatgacat acctcctaag aatttgtact tataagtata ttacatttag acagcttaat
    81361 aataaaggtc aattgacccc aaaaaaacag acatcttgaa aaataagatg tctgttttca
    81421 tcattctata tatggaagaa attttagaag ttccttagga tttataataa taaacttgtt
    81481 atctgaaaaa tcgagtaatc cttgcttttt acattcgtct attgcccgat taacgctcct
    81541 aataggcgaa tttacttctt tagcaagtcg agatttatta agtgaatcaa gcgtatttcg
    81601 atgataatgc aatgatatac tccgaagcaa gcgttctttt actgtgagct ccaaaatgtt
    81661 ttctaccaat tgggaattaa taattagttt ttcggctaat ccttgtatta agaatgttgt
    81721 tgcattaaaa tcatttttaa tccattccag aaaataagtt cgataaagtc gttttacaat
    81781 acaatctgaa agtgctgtaa tttctacagg cttttttcca tcataaaatt gttctatttc
    81841 tccaaaaaaa cttcccgatt tatataagta aagtgttgaa aaattcccat tggaactttg
    81901 aatgtaagct tcagcaacac cagataataa aaaataaacg aaattatttt cttcttcagc
    81961 atataatata gtattcccag cagcatagct tttttcttgt agtttatgtt ttacttcttt
    82021 gggcattata tcaataaaat tcatttttaa atctcaccta ccttcattgt tgatttatat
    82081 ctaccatcaa ttggcttttg ggcatctttt ggtatcatcc acgctcgacc aaatttctca
    82141 gtaccgttaa tgcgattttc ttcacataat ttttgtatcc gtctttctga tatgccccat
    82201 ttttcagaag cggctttcac agaaatgtaa tccataatat taacttcctt tcgcaaaaga
    82261 gtatataatt attatatacg gattgacgaa taatagcgaa tttgtttttc acagatatga
    82321 aattatacac tacctaatat aaaattgtct ttcgttctca tatcaccaca cgaaatgtaa
    82381 aatgatatag aggtgatatt aaaatgtacc aagatgaaag aaaacttgat ttcaagccaa
    82441 taggtatggc gataaaaaag gctcgtgaag caaaaggttg gacacaggaa taccttgtcc
    82501 aactggtaga cctttgtaaa tttacacagg tggtagtttt gcccacgcca cccttttggt
    82561 tggcgatggc gattgttttt gcgttcaagt tttttcctcc tttcgatttt tgggtacaaa
    82621 aaattgccct ttacttaatg taaagagcaa ttcctatggt gtgtttttat attacttcaa
    82681 aatgagcaag tgcatctttg atagcatcta cttgaatagg agaggggtgt tttcttggtt
    82741 gtcttagcgt ttctactgca ttaggagtat ccatacatag gtaagcccaa gctacgcttt
    82801 acttcggcaa tatatgctgt gtgtacctta aagctgtact tctcaagaat atattccttt
    82861 ataagcttgt aagttattct ctgttttggc ttgtatgcgt ctgccttttc tactatacta
    82921 ttaagagaaa tcatgccaac gctgtctcca aagtccacag ttacattgat atagctgtct
    82981 ggagctttgt gagtctttgg taataccgtt gagactgtag ttaaacttat tagagaataa
    83041 cagggtttca gctgtatata tgatgttata aaggtgggtt tgccaccgat ttgccatcgt
    83101 aattgttttt tggtggcaaa tttactctac aatactttca aaaacattta ctgtttggtt
    83161 tctcatgttg ttagttacat gtgaatatgt atccatagtt gttgaaattt tagcatgtcc
    83221 gagtatatgc tggatatctt ttatattagt accattttct aataacatag tatcaagatt
    83281 tttaaaataa agtgtgtatt cttctaaagt agtgataatt aaaatatcaa atatcactat
    83341 acaaaggcag gtgcacgctt tttatgttta acaaaaacga aatattaaaa aaacttattg
    83401 aagaatatga tgtcaaaaca actacatata ttcaaaacat gctaaaagat ttatttacaa
    83461 atacaattca tcaaatattt acattgtcga attttaacgg tttaaacata atatattgac
    83521 tgatataata aaatcataga atattttagg gggaaataga ttatgtttaa gaaaaagtta
    83581 atagctgttg caatgagtat aactatgatt agtgtaggga gcttttcata tgctcattca
    83641 ggaagaactg attcatcagg aggtcatcgt gataacaaaa ataaatctgg attaggaagt
    83701 tatcactatc attgtggtgg ttatccagct catttacata ataatggtgg ttgtccttat
    83761 actggaggag gaagcagttc tggaacaaca acttcggtta ataatgaaga aaagcaaaag
    83821 aggtctgttg gagaaaaagg atataatcaa ggatatgaag atgggtataa aggaaattat
    83881 agttcatcaa attatagtgg agattatagt gatacatatg aatcaaagta ctcagaagga
    83941 tatgaaaaag gtaaggctaa attagaagag gaagaaaagg tggctaaaga gactggatat
    84001 aatttaggga ttacaggtgc taaatctaat aatacatatg aaaaagaagc tttaaaaaat
    84061 gcttatgata ccggatattc aactggatac aatgaatata agactaaaaa gatagaagaa
    84121 tataaagcta aagggataga ggatagcaat aaagataaag aaaaaatgac atttgaagaa
    84181 aacatagatt ctgaatttat agatgcttat aataatgctt atgacgaaat acaagaacaa
    84241 ttaaaaaatg attacactac tcaaggattt gagagtgcta tcaaaggaga aagatttgac
    84301 acatcaacta taggtaatgt taaatatgct aattggttta aagaaggata tgataatggt
    84361 aaggttaagt tacctaaagt aaaagaaagt gtatataatc aaggctacaa tgaagaagat
    84421 tttagtgtac cagatgaaat gaagtctatt gaaactaagt taaaaggtgt atatgatgaa
    84481 gggttagaaa aaagagaaga agagaaaagt agaaatgcta cttatggggt tggagctgga
    84541 acagcagctg tagttgctgg agtagtatat aaaaagaaaa aacgtaaaaa gatatagatg
    84601 ctagatatac caatgatgac aagtgtataa caaatataat tttaagtata aatatttaaa
    84661 atatagaagg gaaggttata tctatgggat ttaactttag gaaaagcata aatctaggag
    84721 gaggactcaa attaaattta ggtaagaaga gtgtaggtat aagtgctggt gttaaaggtg
    84781 ctagagtgtc agttaattca aaaggaagaa aatcagctac attgagtata cctggaactg
    84841 gaatatcata tacaaaaact agtacaaaga aacgtaaacc taaataaata tttgatatac
    84901 taagaaatag actgttataa atagtctatt ttttagtata tagatttaaa aagtgacaaa
    84961 attttgaaat ataaactaat ttcataaagg aaaatattct tttatgtaga attataatat
    85021 ttaagtaata ttataaaaat tataggaaga tttcattatg aaattaatca aaacactatt
    85081 agcaataggg atgtctgttt cattattgat tgctagttgt ccaacatcaa atgctcttag
    85141 ttctattagt tcaataaaag gtacaaataa gtatgaaaca gcaggtttaa tagcagataa
    85201 acaaaattat gatattgcaa tacttattaa ttctgaaaat ggattatctg atggattgtc
    85261 agcaagtgga ttgtcaggag ctaaaaatgc cccaatattg ttaactaaga aagatacaat
    85321 cccttcagaa acattaaaaa gattagaaaa ggtaaaagaa gtatatctaa tcggaggaaa
    85381 aaattcgata ggagcatcag ttcagaaaat attaagtgat aaaaaaataa gtataaatag
    85441 aataagtgga actgatagaa tagatacaag tcataaagtt gcaaaagaaa taggaagagt
    85501 aagtacggtt aaaaaagtaa tattaactaa tgcttttaaa ggtgaaccag atgctatgag
    85561 tgttgcttct atagcagtta gagataaatc tccaatagta ttaactgatg gaaatactac
    85621 aagttttgct acagatggat tagaaactta tgcaattggt ggaaaagtca gtatgagtga
    85681 taagttaata aatgaaacta aagcaacaag aataggtgga gtagatagat acgatacaaa
    85741 taaacaaata ataaataaat tttataatgg ctcaaaagaa ttttacatag caagtggaac
    85801 tgatttagtt tactctttag ttggttctac aattgcacaa aaaacaccta tagtgttagt
    85861 tggaaatgga agcaataaga gtatattaaa aaatgcaagt aaaataactg ctattggaaa
    85921 tattgatgat gttgttataa aacaatgtct aaatataact aataatgttg gggattcaaa
    85981 tacaggtatt gtaaaagata ctaattcaaa taatacgaac aaaccaagta atgagcctag
    86041 ctctaataat cctggattaa ctgataaaag tacagtatat gtaactccta atggaaaatc
    86101 atatcatcta acaaaaaatt gttctacact aaaaagaagt aaaacaataa ataaagtaac
    86161 attaaaacaa gctaagtcac aaggtaaaac agacccttgt aatatttgtg tgaagtaata
    86221 cattagccaa atatatatta aaaaagacaa ttaattaaga ttgtcttttt tat
//
LOCUS       DAEPXF010000002        62002 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain HB27
            SAMN13376389-rid12479423.denovo.002, whole genome shotgun sequence.
ACCESSION   DAEPXF010000002 DAEPXF010000000
VERSION     DAEPXF010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13376389
            Sequence Read Archive: SRR10518432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 62002)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 62002)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001069624.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001069624.1
            Genome Coverage       :: 315x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/15/2021 01:54:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,165
            CDSs (total)                      :: 4,076
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 89
            rRNAs                             :: 10, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 10 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 19 of 69
            Pseudo Genes (incomplete)         :: 44 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 10 of 69
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..62002
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13376389-rid12479423.denovo.002"
                     /strain="HB27"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="China: Hebei"
                     /collection_date="2017"
     gene            203..346
                     /locus_tag="KRL67_000093"
     CDS             203..346
                     /locus_tag="KRL67_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH1521007.1"
                     /translation="MDDDINMIAIRPEDIKVEFNRDFKEVDIKVREILDIEFRRFNYI
                     VTV"
     gene            684..1751
                     /locus_tag="KRL67_000094"
     CDS             684..1751
                     /locus_tag="KRL67_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative 2-aminoethylphosphonate ABC transporter
                     substrate-binding protein"
                     /protein_id="HBH1521008.1"
                     /translation="MKFKKLVSLGILSTLLLSVITGCSNVSSGSNLKSNNKTKGELTI
                     YTAIEEDSIDTYLTTFKEKYPDIKLNILRLSVGNITARLLAEKDNPKADVLWGVAATS
                     LIILDDKEMLEPYAPKGFDKVNPVFKDDKKVPSWVGIDAWMTGITVNTKELKAKNIST
                     PSSYEDLIKPEYKDLISMPSPASSGTGYLTVSELIQIMGEEKAWQYMDKLHKNIGVYT
                     QSGSAPAILAASGEYPIGISFGNRGIKLKGEGYPVEVVFPKEGSGWEIESNALVKKDN
                     IKEASKVFLDWAISKDAMNEYSKNFAVTTIDTGNPVPEGFPKNPLEQMIDNDLKSAAR
                     NREAILNKWIEKYDGKTEKES"
     gene            1943..2473
                     /gene="rnmV"
                     /locus_tag="KRL67_000095"
     CDS             1943..2473
                     /gene="rnmV"
                     /locus_tag="KRL67_000095"
                     /EC_number="3.1.26.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease M5"
                     /protein_id="HBH1521009.1"
                     /translation="MIKEIIVVEGRDDVTAVKRAIDAELITTGGFGFPKGVMERIKAA
                     NERRGVIIFTDPDFAGEKIRKKIASEVPGCKHAFLPREEAKKDGDIGIENATPKSIIA
                     ALNKVRTENTEKRDEFKQVDLIRNGLIGNEEASLRRDALGKILGIGYGNAKQFLNRLN
                     NYGVERDEFIDALKQI"
     gene            2644..3513
                     /gene="rsmA"
                     /locus_tag="KRL67_000096"
     CDS             2644..3513
                     /gene="rsmA"
                     /locus_tag="KRL67_000096"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HBH1521010.1"
                     /translation="MDRLSSHNATKEVVQKYNFKFSKSLGQNFLIDSNIIDKILSGAR
                     ITRGDNIIEVGPGIGTLTREMGKIAEKVVAIEIDRNLIPILKDTLSDLDNTEVVNQDI
                     LKVDIQELVKDKLNGGPVKLVANLPYYITTPIVMKFLEEDIPVTDIVVMVQKEVADRM
                     NAIPGTKDYGALSIAVQYYCETEIVAKAPRHMFIPQPNVDSTVIGLHVRDKRKYDVHN
                     EEIFFKTVKASFGQRRKTLLNSLGGLGFLNKDEIREILKEANIDEKRRGETLSIEEFS
                     VLSNIINTKVSSK"
     gene            3579..4838
                     /locus_tag="KRL67_000097"
     CDS             3579..4838
                     /locus_tag="KRL67_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH1521011.1"
                     /translation="MGSKRKFKRDYIILEAKDINFRYKERVLPKAFAKVEVNDEKAII
                     ALYVENLKYVKDGYKAVAIKEDHGLIDLGNIILNEQGRGEFVLDLEDYDDEIKGIALL
                     YERNIPLIGFKGNKLENYEDILFQEVEEEEYEELEEEDEEIEDNEESDRDEDSEEEEY
                     IIEEYDEDEYEDYWEDDEDIEEGELKARTEVEDSGDEEIEEFNNYKEIENYNRVKVQE
                     EYIDIEYDEEEEDEEEVFEVNREEDRFVSSKPTKSKKVQKDQNNIEKIKRANGVSQGE
                     SGNKQTSTLLMPRQIKKGLKLFKEVKPFVTDYIDKTRWWKIEINPTTLCGYTMPYLGY
                     LNVLNYTMYSDAVLHSYKYRHYLFGVQYDEYNKRKHYIYAIPGNRNEQPDNGSTGFLR
                     YQACDNRTNSLGYWLCFIDSRQRNIVR"
     gene            4989..6104
                     /locus_tag="KRL67_000098"
     CDS             4989..6104
                     /locus_tag="KRL67_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HBH1521012.1"
                     /translation="MINEQRILNEFLELVQIDSLSLKEGNVAKVLVKKLEEIGCSVLI
                     DNAGEKANGETGNIIATLKGNKEGKKILFSSHMDTVTPGIGVKPIVDEANGIIKSDGT
                     TILGSDDKAGIAAILEGLRYINENNIEHTDIQVVFSICEECGLVGAKNLDYGKIDSEY
                     AFILDSGGSPGEIIVKAPAQDVINVKILGKTAHAGLEPEAGISAIMVAARAIENMNLL
                     RIDEETTANIGIINGGTATNIVTGEVNIVAEARSLKENKLDVQTKHMVETFEKAAKDF
                     GAQIEIDVNRAYTPIDVSEDSEIIKLAKKAFSNLGIEGHTESTGGGSDTNILSKNGIE
                     AITLGIGMKNAHTLSEHIAIKDLYDSAKMVVEIIKEA"
     gene            6290..7186
                     /locus_tag="KRL67_000099"
     CDS             6290..7186
                     /locus_tag="KRL67_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HBH1521013.1"
                     /translation="METRYEEANKITIQSILWNVVLTIIKVIAGVIGNSSAMIADGLH
                     SASDIISSIGVLIGNYVSSRPGDREHNYGHEKAETLVSFVLSILLIFVSITIGIEAIK
                     SLFNLDALSVPSILPLVVSVISILIKEYQYRITIKVAKKINSPALKADAWHHRSDALS
                     SVAAFIGIGGSILGFKPLDPIASVVVAIFVAKVGISILISSVNELMDVSVDEEEIKEL
                     KFIVADTEGVKNLGDIKTRKHGAMAYVDLTICVDENLTVKQGHDIATKLEKHIIKHME
                     FVKGITVHVEPCTNCQGNKCNN"
     gene            7256..8053
                     /locus_tag="KRL67_000100"
     CDS             7256..8053
                     /locus_tag="KRL67_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH1521014.1"
                     /translation="MSKKRKLKKKAAILIGVVVFFIIYFISFRIGINMEKDKSEVPKK
                     VEKEEEDNRSLRTQIKEKKKVYISDKNIENIKIEEDMWDEIKLFFSDFKKVRNTTSSY
                     EAVYEGYSDDGVKFSTDLNFFRVYTVNKEEYYKVPVSSKDAFKKLLDESIYTSFDLLS
                     HYKDWKEVTITHGKEIKKVHRWKFDDLAHKMISKRVVGKVQPEKSKERSDYNFTINIK
                     GDNFDVKVDTMGKDYVKVTSKKNVSYYEVHTSLFDYLKNEIFEIGKK"
     gene            complement(8108..9460)
                     /locus_tag="KRL67_000101"
     CDS             complement(8108..9460)
                     /locus_tag="KRL67_000101"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HBH1521015.1"
                     /translation="MNIHFIGIGGISMSALAEICINKGYQVSGSDSNESYLLDKLRDQ
                     GATIYIGQKKDNISDDVNMVVYTAAVHPDNEELVAAKEKNKLVMNRATFLGQIMREYK
                     NSIAVSGTHGKTSTTSMLSTIFEYADLDPTILVGGNLSMIGGNVKIGNSNHFITEACE
                     YVDSFLNFNPKISIVLNVEEDHLDYFSGIDEIKASFNKFGKLLPPEGYFIINGDDENI
                     DDILYDVKATIIKYGRDSDNDAVIKDIHFDNSGHGIFRIEYEGRDLGEFELSVYGLHN
                     IYNASSAIMAALVSGIDLETIRKNIKIYKGVGRRFETKGYYKNALVVDDYAHHPTELK
                     ATLAAAKKLKKSTLWCIFQPHTYTRTKSLLGEFSEAFYAADKVIITDIYAAREKDPGD
                     IHSKDLVEKLYQNNVDAIYIKEFEDIVKYLRENVKDNDLVITAGAGPIYKVADLLVEK
                     "
     gene            9679..10557
                     /gene="purR"
                     /locus_tag="KRL67_000102"
     CDS             9679..10557
                     /gene="purR"
                     /locus_tag="KRL67_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pur operon repressor"
                     /protein_id="HBH1521016.1"
                     /translation="MKFKRTERIGAIVKILSDNPNKIYTLSYFTNQFNAAKSTISEDL
                     LVVKNVFEKLHLGKVITISGAAGGVKYIPKTSIAENAEFLMELCERICDKSRILSGGF
                     LYLIDLIYDPTIAAKIGKIFASNIEYVDADYVVTMETKGIPMALMTAKAMNLPLVIIR
                     KDIKVSEGPTLSMTYVSGNSSKVESMSLPRKALKPDSKVIIIDDFMRGGGTIKGMVDL
                     MNEFGAEVIGTGVFISTTNPSEKMVKDYISLIQLDVNGDKIVVEPNLKTFKDEYRNED
                     LDEEEDSEFEFEIDED"
     gene            10713..10991
                     /gene="spoVG"
                     /locus_tag="KRL67_000103"
     CDS             10713..10991
                     /gene="spoVG"
                     /locus_tag="KRL67_000103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation regulator SpoVG"
                     /protein_id="HBH1521017.1"
                     /translation="MKITDVRVRKLTEEGKMKCIVSITFDNLFVVHDIKVIEGHNGLF
                     IAMPSRKVGEGNFRDIAHPINAEMRQVLEDAVLQAYHEALVQWEVAAE"
     gene            11204..12583
                     /gene="glmU"
                     /locus_tag="KRL67_000104"
     CDS             11204..12583
                     /gene="glmU"
                     /locus_tag="KRL67_000104"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HBH1521018.1"
                     /translation="MNFKAIILAAGKGTRMKSKYPKVIHKVCGKEMVNHIIDVSKKSG
                     VKDTVVILGHEADVVKEKLAEEIIIAMQTEQLGTGHAVKMAKEYINDEDTIVVLCGDT
                     PLIKEETLKRLFEYHIENKYHATVLTTRVGNPTGYGRIIRDKKGDLLKIVEQKDANSE
                     EKMISEINSGIYCFNGKSLREALDLLNNNNSQGEYYLTDTAKIMRDKGLKVGAFAGST
                     IEELMGVNSRVELSKAEEIMRRRINESHMVNGVTIIDTNSTYIESDVMIGNDTIIYPG
                     VMLQGKTRIGSDCIIGMNSSITNSEIGDGTEIKNSTIIDSKVGENSTVGPYAYLRPKS
                     DLGNNVKIGDFVEVKNAIIEDGSKASHLSYIGDAHVGKNVNIGCGVVFVNYDGKNKFK
                     SVVKDNAFIGSNSNLVAPVVVEEKGYIATGSTITHDVPDGALAIARERQVIKEGWVEK
                     KNQKDDQSK"
     gene            12678..13628
                     /locus_tag="KRL67_000105"
     CDS             12678..13628
                     /locus_tag="KRL67_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003361732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HBH1521019.1"
                     /translation="MNTSGSEIKIIAGNSSKELAQKIADYIGVSVLDCEVGTFSDGEI
                     CVNMNETVRGCDVFVVQSTNSPVNDNLMELLILIDALKRASAGRITAVIPYYGYARQD
                     RKAKARDPITAKLVANLITAAGADRVLTMDLHAAQIQGYFDIPLDHLLGGTILANYFN
                     EKKIEDLVVVSPDLGSVTRSRKFANTLNGEVPIAIIDKRRPKANVCEVMNLIGDVKGK
                     NVILLDDMIDTAGTIVNAANALKEFGAKDVYACCTHGVLSGPAIERIANSEISELIVL
                     DTIQLPEEKRIDKIKIKTVAPLFGDAIRMIFSNESVSKLF"
     regulatory      14469..14554
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01786"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class II; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01786"
     gene            14716..15237
                     /locus_tag="KRL67_000106"
     CDS             14716..15237
                     /locus_tag="KRL67_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HBH1521020.1"
                     /translation="MKLKKNKKGFTLVELLVVIAIIGILAVVAVPALFSNINKAKVAS
                     VESDYSSVKSAALSYYSDTNKIPVTPDGQTGLNVLETYMESLPDKADIGGKYKLIKVG
                     NKLVLQIGKDGEGVTLTEAQSAKLLSDIGKDKIYTGVTGDNFGDQLKDTTKIDNKALY
                     IVLIDNTVMDSTK"
     gene            15389..17065
                     /locus_tag="KRL67_000107"
     CDS             15389..17065
                     /locus_tag="KRL67_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II/IV secretion system protein"
                     /protein_id="HBH1521021.1"
                     /translation="MAKKVRIGDKLVEKGYITEDQLKWALSEQKNSGKRLGEFLVQEG
                     LIDSNLLISVLKELLDIESIFLEGTEIDTLATKMVPENICKRYTVFPFKIDGNKICLA
                     MSDPQDREAVQDVRRMSGKDVEIFISSTEDINKAIGHAYAHSEINKAMTEYNKNRTGG
                     VRETVILEEDVNAAPIVRLVNNILENAVRMEASDIHIEQSENYMRVRFRIDGMLREYM
                     RMNSAPYKAVISRIKIMSDINISEKRIPQDGRIYLKVDNKPIDFRVSTMPTNRDEKIA
                     MRVLDKSNFMVSKEVLGIDEHGSKIYDELINTPYGLILVVGPTGSGKTTTLYSMLNQL
                     NTENRNLLTIEDPIEYELPGVNQSQINEKAGLTFASGLRAFMRQDPDIIMVGEIRDTE
                     TAEIAIRASLTGHLVLSTLHANTAVGAISRLLDMDVESFLITSSVLGVISQRLTRKIC
                     EHCKVSYEADIGEKKALGIGVNESITIYRGKGCERCNNTGYKGRLGIFEMLEITPEIK
                     ELIDSSANQREILKMARKQGMVSLKEDIVKKVLNGKTTVEEMIRIILMTD"
     gene            17077..18285
                     /locus_tag="KRL67_000108"
     CDS             17077..18285
                     /locus_tag="KRL67_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HBH1521022.1"
                     /translation="MKSFKYTVIKEDGKKQTDVIEANDFNEARNLLRKRNLRVIEIKE
                     SKNKNIRLSSRKRKKDLGADQISHFCRQFAIIVSSGINSISGLETLARRSTNITLREE
                     INRIVAEIKVGSTIADSMLSSKSKFPKLLGAMVATGEATGKLDEVLKSMASFYASEHR
                     VKQKLRNAATYPLIVLISSFIMIFIFTTFMVPKMINSITTVGATLPLITRIVMGFGMF
                     MKKFWIIVLLVIILFIYQFKKYLKTPVGRAHKDRVINKIPVLGKGINCMVATRFSRAL
                     YLFVSTGYPLVQGLDYIIDSVNNTMAEKILVSAKDGITRGEGLAENLERYTYFDSVLV
                     QMIAIGEQTGELENISRQMAEFYEYESEIYLNRMASMIEPVLIIAVGIIVAILVVSIF
                     MPMLSIYDAM"
     gene            18300..20012
                     /locus_tag="KRL67_000109"
     CDS             18300..20012
                     /locus_tag="KRL67_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH1521023.1"
                     /translation="MNKVYVSYYGDSISIVEGRYKHDKDKFYVKNFNVFSIEDIAQDF
                     SKEKYALLRYALENVKLKSRHVVFCLNTRDVILKPQKLPNMDKKDLDGFMKLEMDEMM
                     ALESDEYVFSYEVSSEIPDNSDGDESSLNLIIGAIQKEEVNAIVEIMHEFDLILDRID
                     TLSTAYLRMLKNLDYNDIMVTNISDNSTIINIYKKDTLFISDNIPIKITAKDMDMQLL
                     NVVEESKGLMNYYSSRNFGKVTDTILILGKRYSNKLIYETFSSAFSSYVSQGLTEIMD
                     ISDMITGDIDEDDINSIVELLGSMIEPKGKRDFENINFLPMDILRKQLKAKQMKNILK
                     IAPIVILILAIPYLCLIFSNNLANKELENVNGEIRNIESAYYQIGNIEKMIQSKSEEI
                     KVYNMLIGKKVKWENLLDDIDKNTPSKVELTSLSTTYQNLGTNNKADNSKSNKTTADK
                     SSNNEIKSNTKSDSNGDKTNNDLNEDKTTNVSEALTGNSETNSNNTDKDDKTPLYDKI
                     PNVINIEGNAKDSSYVGQFLYKLKGLYYFEDVKLHSIKSNAEDSNYTFSMTLYLKEGV
                     LTNE"
     gene            20005..20913
                     /gene="pilO"
                     /locus_tag="KRL67_000110"
     CDS             20005..20913
                     /gene="pilO"
                     /locus_tag="KRL67_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 4a pilus biogenesis protein PilO"
                     /protein_id="HBH1521024.1"
                     /translation="MNKDIFKKDIKDIFKMEVSLKGILIKVIILLAILSYGYFGLYPQ
                     YQKYIATKNSVKQATEKLNIYKEKTALMPKYEKQLEEVESELKEKSKNLLYDMQDGLF
                     LIGLDKKMKAYNIDLTSYDVGNVREYEHFYGVSTTISVRGDYRNVKKLMAYLEEQKNV
                     TQILDYTMTSYIEPEKEEEETTNNKDLIKNVNINKDTKIYWVEGENKWHLYKDCPLIK
                     EGEILKNDKLLDRSTSYLCNTCVLRAATEKEAKKEVVAPKAEGVVDATFKFIMYTKEN
                     TTMILDTDNPSSWRPGKYNPFKSSAK"
     gene            20940..21506
                     /locus_tag="KRL67_000111"
     CDS             20940..21506
                     /locus_tag="KRL67_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HBH1521025.1"
                     /translation="MSLYKNNEKGLTLLEVIIAVFILTIVLSISYKVFNGITSAVKRQ
                     QIITDAQVNINLINKYLNRDLENCKELTKTGSGNNYEYNIEMPDNVVKYEVSIETKKH
                     TEVYSVTRIQKNTIDTENEVREEIIYNQPLVQNNKEMKETPFKIEKQTGKSIYTVSIY
                     YNESVQESHKNINLNNKTYTFDVMSRIG"
     gene            21543..22070
                     /locus_tag="KRL67_000112"
     CDS             21543..22070
                     /locus_tag="KRL67_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HBH1521026.1"
                     /translation="MSKKNSERGFSLIEVLVAMAIMGIVLFAFFNIINTNNKANTKND
                     TDITSLNYVQSEIENLREKIKSGEFDFDSLDKLEDGTVVYEKLIDKSKKVVYDKVLSE
                     SDVSLYDIPYEKITTIKDEDGNLIDKENITNKIKTIVEDKSGQIYKVAVTGKSMNDYS
                     SKKEVKIVTEIFKDK"
     gene            22091..23629
                     /locus_tag="KRL67_000113"
     CDS             22091..23629
                     /locus_tag="KRL67_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH1521027.1"
                     /translation="MRKWNKFKSERGAALVLVLVVVALLSIVGLIFSNQIANRIKSTK
                     TTNEGIQAKYLAETCVENSIDKAYEKLYDELEKMDNEFKSENQEKSISRSKLRNISDE
                     DFNNQDEENIEAERLGYMNNINFYLNKASSELEKASMELKKLYDLDMLDYRDIEYVDA
                     NIISHRDSILEICKNYTSGDISKINEHILKEDIDSTTLIEAKLVNNDILLKMFLEENK
                     IENEHLNSAFSHTYKALDNISLAMQNMIEYRHTFHIDEPKVEVSNGIPNSQEYYELIQ
                     NPIINSMEYSWNSKWDTLENLLEILPNQTQGFNSLRVHLRNNVRKFEKLSDNISSGKK
                     NTAKNFLKYKELLYEISDQCNQLKSMSYEKIPVKYDNMALITTFDYIQNELLAEIKCR
                     LKELKPQEVDKTEGISIKIPFYKADYDMTKEGWPKLKENGSGAELSLMVTGDKDGIKE
                     VEVTDSKKNIIGLGVEENSNSKYKVEAIVNFNLNIDTNVVGNYNIKDKILINHDISSY
                     KKVN"
     gene            23729..24811
                     /locus_tag="KRL67_000114"
     CDS             23729..24811
                     /locus_tag="KRL67_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HBH1521028.1"
                     /translation="MYIYDLLEQGIRLNASDIHITVGTNPVARVKGGFVKLSEQILTS
                     EVTMQMAKDIAGESMFKVIEEHGEADFSASLKTGERFRVNAYRQKGNYAIAIRTITAE
                     IPTFEKLGLPENIKSFTEKHKGLVLVTGPTGSGKSTTLASMINIINEKQQKHIITLED
                     PIEYVHHHKQSLVNQREVGTDTESFHSALRAILRQDPDVILIGEMRDPETVSIALTAA
                     ETGHLVFSTLHTVGAAKTIDRIVDMFQPSQQQQIKTQLSTVCEGVVSQQLLPTADGKG
                     RIAAIELMFATPAIKNLIREGKTYQIPNMIQTGVKSGMKTMDQDLMELYKNGKITKDM
                     ALSRCTDQEFMTRMIGGVNYNGYYNR"
     gene            24792..25574
                     /locus_tag="KRL67_000115"
     CDS             24792..25574
                     /locus_tag="KRL67_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HBH1521029.1"
                     /translation="MDIIIVNLYVFIVGIVFGSFFNVCIFRIPEGISISRPPSHCMSC
                     NTRLKPIDLIPVLSYLMSGRKCRYCYEKISSRYAIVELITGILFLTVFMLYGISTSTI
                     YYLVLVSLLIIITFIDIDHFIIPDKILIFGAIFSIIFNLLFKEIPVKDSIIGVFICGG
                     SVWIIVLLIEFVIKKECMGGGDIKLFAMLGLYMGVKNGLLTALLSIYVGAVYGICVII
                     TSRIKGKEYNSVIPYGPFISIGALITILCGKQLLEFYFSMVI"
     gene            25676..26326
                     /locus_tag="KRL67_000116"
     CDS             25676..26326
                     /locus_tag="KRL67_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HBH1521030.1"
                     /translation="MEYIIIISLFFAHLINKSLCVFCDREKKNLYNTFFIYIITVVLS
                     VLIYLKFGISVECVQYMVLIPFLIIISIVDYYTIYVYDITIVSGIIVQGIIFLVFFNI
                     VQFDYINHIFGLLIGFIIPYIIAKLTKGLGYGDVGLYSLCCFALGNNYGFYVIVLSFI
                     LAFLFFIILYITKIFKDVDIRKQIPFTPFISLSTILIIFTEYNIFNFYYSLLYKFI"
     gene            26340..26900
                     /locus_tag="KRL67_000117"
     CDS             26340..26900
                     /locus_tag="KRL67_000117"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HBH1521031.1"
                     /translation="MYVVVGLGNPGKKYEKTRHNVGFDVIDILAKEYNISVTKIKHKA
                     LIGEGRVGTEKVLLVKPQTYMNLSGETLIDIYKYYKIDLSNIVVVYDDIDLEVGKIRI
                     RKKGSGGTHNGMKSITKCLGSNDFPRVRVGVSKPEVGQDLADFVLSRFRKEESDNINE
                     ALEKAAYAIDSIIRENIDMSMNKYNG"
     gene            26917..30303
                     /gene="mfd"
                     /locus_tag="KRL67_000118"
     CDS             26917..30303
                     /gene="mfd"
                     /locus_tag="KRL67_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HBH1521032.1"
                     /translation="MNDVFLYPLQNSKEYKDIINCIKNTKGSLLVNGLLPVQKPHISY
                     SIFNDLSRQMIFITSSDLEAKKVYEDLSFYMEDKVEYLGFQDIYFYHLDAKDRNEEAK
                     KLKVLLKLANKEKIILVTSIEAVLRKYIPKQVLLDSVSHYKVGDSLDLEKLTEKLVSL
                     GYERVSKIEGFGQFSIRGGIIDVFSLEYTNPIRMELFDDEIDSIRTFDVYSQKSIDKL
                     QQFSITPSREFIYPEKTTDALVKLKKETTKNTDEGTFQNIDYISSKTYFEGVENYIDY
                     IYPEENKSIFTYLADDAIVFINDITRLKERCENYINEFRENYKLNLERGLAIKNQGKL
                     LYHYTDLEYLVKDKSVVLNSLLPKSINNFSIKSIINFESREVPTFNGKVDLLVEELNR
                     LKYNGYKIILATNTLERANKLGKDLLDKGLETTISKDRDIEIKSSQVIIVPAHINSGF
                     QYKSIKFVVITDNEMIGVYKRASKTSNKKVKKGKKIESFLDLSVGDYVVHENSGVGRY
                     TGIEQITVNAIKKDYMKIVYQGGDNLYVPIDQMDKVQKYIGAEVEKVKLNKLGTNEWT
                     KAKAKVKKEIEDMTKDLIELYAKREKIQGYKFSKDTPWQAEFESLFPYQETEDQLKAI
                     EETKKDMESSKVMDRLVCGDVGYGKTEVAIRSIFKACMDQKQVAVLVPTTILAQQHYN
                     TFKERFENYPLRVEVLSRFKTPKQQKQIIEDAKKGLVDILIGTHRIISKDINLPNLGL
                     VVIDEEQRFGVKHKEALKKIKSTVDVLTLSATPIPRTLHMSLSGIRDMSVIEEPPQER
                     HPVITYVTESKESVIQDEIERELSRGGQVFFVYNRVEHIEEMASMIQKLVPDARVAVA
                     HGRMTSKSLENIILGFLNKDYDVLVCTTIIETGMDISNANTMIIYDADKMGLAQLYQL
                     RGRVGRSSRQGYAYLLYEKDKTLSEIAEKRLKAIREFTEFGSGFKIAMRDLEIRGAGN
                     ILGSQQHGHMAVIGYDLYVKMLNDAIKKVKGEPIVEEIDVEIDLSVNAYIPDNYIKDE
                     LIKIEMYKKIASIENKEDMLDIQEELEDRFSDIPKPVQTLLTIAYIKSLCKILKIEKI
                     RQLKDEILLVPITKYRTKQKIGYNIVTELEELLEKMCKVK"
     gene            30334..31329
                     /locus_tag="KRL67_000119"
     CDS             30334..31329
                     /locus_tag="KRL67_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HBH1521033.1"
                     /translation="MKKVITLVITMILVVSVTACSSSKGETVATVEGTKISSDEFKKT
                     IALYKDSMEQTYGKDIWDKEVEKGVKYKDKFKDLILDQLITTEVIYSQAKKDNLLPKK
                     EDVEKSFKELKDAMGKDEKYKEQLKKLGIDDEFLRDQQEKDLAMQNYQSNFAKKTKIS
                     DEEMKKYYDTHKDEFKKDEVEASHILLKTVDDNNKPLSDKEKAEAKKKAEEALKEVKS
                     GEDFAKVAKKYSQDASASDGGKLGFFSRGQMVAEFEDAAFSMKKGEISDLVETQYGYH
                     IIKVTDRINEQTSFEDAKETIKEQLLKNKYQEQIEKLTKEAKVEKDEKVINKITI"
     gene            31453..31992
                     /gene="spoVT"
                     /locus_tag="KRL67_000120"
     CDS             31453..31992
                     /gene="spoVT"
                     /locus_tag="KRL67_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein T"
                     /protein_id="HBH1521034.1"
                     /translation="MKATGIVRRIDDLGRVVIPKEIRKTLRIREGDPLEIFTAKDGEV
                     ILKKYSPIGELNEFSQEYAETLAEVLGYGVLITDLDSIIAVSKLPKKDYKEKSISDEL
                     EAIISERTVKYSKDKNILPLFKEDQKEYTSQVIMPIISASGDCIGSIVVVSKDKDTIA
                     ESEEKSLKIAANFLGKQVQ"
     gene            32112..33725
                     /locus_tag="KRL67_000121"
     CDS             32112..33725
                     /locus_tag="KRL67_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HBH1521035.1"
                     /translation="MNNNKNKDSFLKGALILGLAGVIVKIMGGFFRIPLGNMIGSEGM
                     GYYQAAYPVYTLFLTLATAGFPTAVAKLVSEKVAIGNFKGANKIFKVSHTVLFITGII
                     SFCILFFGADYIVTNVMKNPGALYSMKAIAPALLFVPAMSAYRGYFQGRQDMTKIAVS
                     QVAEQFFRVVLGLTLAYLLMKSLGQTFGAAGAISGATIGSIASMLYLVFAYMLGRKER
                     RAEIDASQRFKDERVSYIFKKLLTVAIPITIGASVMPLVNMIDNVIVIRRLMEAGFTY
                     KVANSMFGQLTGMAMATINLPAVITTAMSMSLVPAISKAYALGNKSKARKDTKSAVKV
                     TLLIVLPCAFGMASLAIPIMGLLFPHEPSSVGTILFTLTPCVLFLGLIQTLTGIIQGM
                     GKPIVPVIALCVGMLCKIVISYTLTGIPDINVLGSAFGTVTAYFVAAMINLLYVKKHM
                     NVNFSKKEFIIKPFITVMTMFIMVKLSYGALVGFLGNSISTIIAICVGGIVYVVVILG
                     IGGIKKEEILTMPKGDKLYKLLKKVKLIR"
     gene            33832..35280
                     /gene="mazG"
                     /locus_tag="KRL67_000122"
     CDS             33832..35280
                     /gene="mazG"
                     /locus_tag="KRL67_000122"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HBH1521036.1"
                     /translation="MGKISIVGLGPGDYSLISQGVLESLINSPKVFFRTEKHPIIDKL
                     KETIQYTSLDYFYEKEENFDNVYLKISEFIIEESAKGDLVYAVPGHPRVAEKTVSIIE
                     RLANEKHIEVEILPSMSFVDAMFNYLAIDPSEGFKLVDAFEIENSYIDLDTSMIITQV
                     YDKFIASNVKLKLMEHYDYNQEVCIVNGAGVKDLESKKFIKLCELDRNENFFDYLTSL
                     YIPKSSKKMYNTVHDLEIIVNTLRSPSGCEWDKKQTHQSLKNSVIEEAYELCNAIDNN
                     DIDEMVEELGDVLLQVIFHCQIGNEEGYFDLKEVVNGICKKLIHRHPHIFSGAKLDMS
                     KFEKTWEELKKEEKGESTITDGLRRIPKHLPALMKASKVQHKAALVGFDWDNVEDVFK
                     KIEEEYQELLDEHRVGNIKYIKEELGDLLFSIVNLARFLDIDSGEALNCTTEKFINRF
                     DFIEKSAMNLNKKIEDMTLEEMDEFWNQAKKQ"
     gene            35369..35647
                     /locus_tag="KRL67_000123"
     CDS             35369..35647
                     /locus_tag="KRL67_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003153447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HBH1521037.1"
                     /translation="MNKAELVSKMAEKSGLTKKEAEAALNAFMSSVQDALVNNEKVQL
                     VGFGTFETRERAARQGRNPRDPEQVIDIPASKAPVFKAGKGLKDIING"
     gene            35719..35961
                     /locus_tag="KRL67_000124"
     CDS             35719..35961
                     /locus_tag="KRL67_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HBH1521038.1"
                     /translation="MRLDKFLKVSRIIKRRTVAKDACDKGIVTINGKVAKSSSEVNIG
                     DTLEIQFGEKKMKFKINEVKEHVLKNDAKEMYEILE"
     gene            36023..36277
                     /locus_tag="KRL67_000125"
     CDS             36023..36277
                     /locus_tag="KRL67_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation protein YabP"
                     /protein_id="HBH1521039.1"
                     /translation="MEQNITLKDRSKLVISGVEHIYSFNDKRVELKTSVGEMVIEGEN
                     LDMSKLSIDENIISIDGTINSMVYAKPKKPQESFLKKVFR"
     gene            36278..36811
                     /locus_tag="KRL67_000126"
     CDS             36278..36811
                     /locus_tag="KRL67_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH1521040.1"
                     /translation="MIPFTQDVSIFYATIYGGILIGVLFDFYRGLRGNFKFINYFAII
                     FDVLFWFLATVIIFVTINLTEFFDLRYYHFVALFIGFILYYNTISKIVLSIINKIIRF
                     VRNSFKKVTHYIVSFLNNLYYVIIYSLHLLFDIIFYIPNIFIATRKSIKRKSNKKLKN
                     KKKSKPKKKKNKTKKRV"
     gene            36817..37101
                     /locus_tag="KRL67_000127"
     CDS             36817..37101
                     /locus_tag="KRL67_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HBH1521041.1"
                     /translation="MNLRKKFSGQIIVISLFLGISIFSMMTGFVFEYTKAKEYKKEIA
                     SLNKQLKKTEIQINNLKKDEKSYEGDLEDIARKRLNMVKPNETVYVDINR"
     gene            37214..38134
                     /locus_tag="KRL67_000128"
     CDS             37214..38134
                     /locus_tag="KRL67_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ppx/GppA family phosphatase"
                     /protein_id="HBH1521042.1"
                     /translation="MKVGAIDIGTNSMRLLIADYRDGNFINRKKLVNTTRIGYGVDSK
                     GYIKEDAIKRNIKALKEFSDKCSDEKCEKIYCMGTSALRDSKNGDYFVKLAKEETGID
                     VDIVTGIEESNLGFKGVLEGIQGDDTIIVIDIGGGSTEFIVGDSEGIKFNKSENVGAL
                     RMTERFLKHDIIEDEEFEAMSSFIYDEIDKTLNHIKDRGLKTLIGIGGTITSLSAMNQ
                     ELEVYSMEKIHNSKIHKKEIEEILQKLIKMTLSDKKNLKGLQEKRADIITAGVKILNI
                     IMEKLEIEEITVSEYDNLEGLICQKSKKMY"
     gene            38224..40596
                     /gene="spoIIE"
                     /locus_tag="KRL67_000129"
     CDS             38224..40596
                     /gene="spoIIE"
                     /locus_tag="KRL67_000129"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein E"
                     /protein_id="HBH1521043.1"
                     /translation="MQRSVAVVKNKTVKPNVIKLISGYIDFTTAAFAVMGFLLSRSII
                     VDSVAPLGIAFFICASKIDKYKIPVFISTLLGILLSSNDFGNVAKYAVCLTIFMAMNK
                     KLKLSDSIAKVAFIGAIILLPISMGQAFFSNRYVYGLLIAGVEAIIMFISIYVFSYGI
                     GLMANVNNRMSVRTEEAISISLLLTFSIMGIGDISLFGISIRTILSTVLILLTSIVGG
                     ATMGASSGVIIGVASILNNMTSAIYMGIYSFSGLISGAFNKINKYFCILGYILSWTII
                     YLYTSGITSNMMQLRDILLGCLIVLVLPERLFNKIEKLIKSNVASNEIVYDYIMRSKN
                     LTNSRLNSIYKTYDDLADTFDKIREKDKVLDQRDIANVIDMIHNDECKSCSMRRMCWE
                     SRFNHTYTMIYNILEKIEEKGELSLNDIPKNFRKECMKPESIVKISNHYYKMFVLDYD
                     WSVKFSESRKLIANQIRSISKSIKSLSQDLEGDIMLDIEKEKNIYEQLERYDITVDKV
                     SYLTKSNSEFEISIEKKTCHDGCMCEDKIVNIISDLVGENMSVRKIGCHCLGGKCKAT
                     FVKSQKYKAVTEVSAMSRDGHILCGDNYTYMEINDGKYMMAISDGMGKGKKAYEESSA
                     TIDILEKMIDAKIKDEIVIDTINNMLLLKSSEEMFSTLDLGILDLKRGCLETIKMGAC
                     STYIKREDGEVDLISSSSLPVGILSDVKIDRKNVKVKEGDYVIMVSDGIVDAGRNNNL
                     GDNWLIYFLKNIETTNPKEISNLILDRALELQALQIEDDMTVLVTKICTK"
     gene            40947..42839
                     /gene="pepF"
                     /locus_tag="KRL67_000130"
     CDS             40947..42839
                     /gene="pepF"
                     /locus_tag="KRL67_000130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HBH1521044.1"
                     /translation="MNRKLCKAIIPLFISIFCMLIISIKADQGYQKNNDRAITVMNNN
                     DTEDDAEKKQEIDYEKTCWNLQSLFKSDDQWKKELKSFDKDTKELKNYIGKVTKSPTH
                     LSFALNIKEKLDIRLNKLSAYVKLKQDTNKNSYKYLDMNDSMSKSYGNYMGICSDLEL
                     EILKLSNKDYKKIISNKNINRKYGAYLRDIRRNKVHYLDDKSEDILSKVSSISSLPSQ
                     VYELFRNMDRKTNLTPAQYASELESSDRENRKKAYQDELITYNDNINTIAGLITGQVK
                     KNIFYSQERGYESSLEMYLESDNVDEKVYNNLIRTVNSNMDSLHKYIDLRKKVLKLDK
                     VHSYDMFVPMVKAVDNNINYEKAQSIVYSALSPLGKEYNDVVYKAFNEKWIDVYSHEN
                     KVSGGYCLSVYENHPYILLNYNNSLGSVSTLSHELGHAVYEYLSSKNQNYFNSNPSIF
                     THEVASTTNEALLYEMLIKEAKGNDEKAYYITQYLDLIKDTLYTQTMYAEFEKTIHEL
                     VEKGKSVNALVLNDAWGQLLKKYYGQSYELDQLSKVGWARIPHFYNSFYVYKYATGCS
                     AGVSFAQDILKNGSDNYMSFLKKGSSDYPINLLKSSGINLTNTKPIENTIKKFDSLVA
                     ELEKLL"
     gene            42953..43918
                     /gene="galU"
                     /locus_tag="KRL67_000131"
     CDS             42953..43918
                     /gene="galU"
                     /locus_tag="KRL67_000131"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HBH1521045.1"
                     /translation="MQVKVKKAVIPAAGLGTRFLPATKAQPKEMLPIVDKPTLQYIIE
                     EAVASGIEEILIITGRNKKSIEDHFDKSVELELDLEKKGKKELLEVVQNISNMINIHY
                     IRQKEPKGLGDAIYCARHFIGDEPFAVMLGDDIVDNDVPCLKQLTDAYEEYRTTILGV
                     QKVNQEDTNKYGIIEAKNIEGRVYKVKDMVEKPESGKAPSNIAILGRYIITPEIFDIL
                     KDLPPGKGGEVQLTDALKILSKKEAMYAYNFEGKRYDVGDKLGFLEATVDFALKKEDL
                     KEDFIKYLKHVCSEFDKNNNVLYNNTDEKIDLIEANGARELEVQK"
     gene            44418..46022
                     /gene="rho"
                     /locus_tag="KRL67_000132"
     CDS             44418..46022
                     /gene="rho"
                     /locus_tag="KRL67_000132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HBH1521046.1"
                     /translation="MRRIFLILDDKVLEGLGSKTLVELREIAKELKIKSITTYKKNEL
                     IEIINSKSKNEEKVNEETKEKNEDIKEDIRNRKENIERESIEKEVEYKSPTKSYNKKE
                     IDTQNISQNQENRNQRNDYNKTTSVNDSNKSSNISNRMVRNNNKNYYMPKQVDESKIV
                     DEFNTSKEDEVVGVLEILPDGFGFLRGSNYLSTEGDVYVSPSQIRRFNMKTGDKIKGI
                     TRHPKSGEKFRALLYVQKINDENPDTAIQRNAFETLTPIFPEERLTLETNRNEIATRI
                     IDLISPIGKGQRGLIVAPPKAGKTVLLKSVANSIAKNHPNVELIVLLIDERPEEVTDM
                     KESIEGDVIYSTFDQVSSHHVKVAEMVLNRAQRLVEHGKDVVILLDSITRLARAYNLT
                     ISPTGRTLSGGIDPGALHGPKKFFGAARNIRQGGSLTILGTALVETGSRMDDVIFEEF
                     KGTGNMELHLDRKLAEKRIFPAIDIYKSGTRRDDLLLDDEEKTALWRLRREMSNNSVM
                     EITDKVIELIKRTKDNKEFVKSIKNL"
     gene            46175..46375
                     /gene="rpmE"
                     /locus_tag="KRL67_000133"
     CDS             46175..46375
                     /gene="rpmE"
                     /locus_tag="KRL67_000133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018591942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HBH1521047.1"
                     /translation="MQKEIQPKYNPVEVRCACGNTFVAGSTKDEIKVEICSECHPFYT
                     GKQKNIEKGGRIDKFKKRFKMD"
     gene            46520..47413
                     /locus_tag="KRL67_000134"
     CDS             46520..47413
                     /locus_tag="KRL67_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1385 domain-containing protein"
                     /protein_id="HBH1521048.1"
                     /translation="MAKQAVGGQAVIEGVMMQSKDKRAVAVRKSDGEIALKEDRIKSW
                     VRDKNIDKIPFIRGSFVMIDTMIQGIKSLNFSSEFFMEEAEEDKFDLFIKKIFKDKAN
                     DIIIIFSLVIAMLLSAGLFIFIPTLVGGAFSKVMPNDFMLNLIEGIIRIAILFAYIVL
                     ISRSKDIERVFQYHGAEHKSIYCYENDLELTVENARKFKRLHPRCGTNFLFIVMAVSI
                     ILFAFFGWPNPILRIFMRIICVPIVAGLSYEVIRVLGKYDNGFTKIIAYPGMMLQYFT
                     TKEPDDEQLEVALEALKAVVD"
     gene            47431..48279
                     /gene="prmC"
                     /locus_tag="KRL67_000135"
     CDS             47431..48279
                     /gene="prmC"
                     /locus_tag="KRL67_000135"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HBH1521049.1"
                     /translation="MTIKDIIIKYSDKLKDISDTPRLDTELLLQKTLGVDRLYIHLNL
                     NKELTEEQKTKFIGFAEERLNGRPIAYIVENREFMGLDFFVKEGVLIPRPDTETLVEE
                     IIEICREKKDVSILDIGTGSGAITISLAKYIENSKIMSFDVSEIALEIAKKNSIINEV
                     GEKIKYINSDLFTAISDSNIKFDIIVSNPPYIKKQDIETLHKQVKDYEPYNALEGGED
                     GLDFYRRITEQGKKYLNKRGILAYEVGHNQAEDVINIMKSNGYKKIYTKKDIQGIDRV
                     VIGYNI"
     gene            48323..49387
                     /gene="prfA"
                     /locus_tag="KRL67_000136"
     CDS             48323..49387
                     /gene="prfA"
                     /locus_tag="KRL67_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HBH1521050.1"
                     /translation="MLKKLEVLEDTYKDLSEKIGDPDVINDQKVWQKYIKEHADLEPI
                     VMKYREYKSVLDSIKESKEILQEESDEELRELAKMELAEMEEKVAPLEEEIKILLLPK
                     DPNDDKNVIVEIRGGAGGDEAALFAGDLFRMYSRYAERRRWKIELLSASDTGVGGYKE
                     VSFMIKGKGAYSRLKYESGVHRVQRIPSTESGGRIHTSTSTVAVLPEVEDVEVEINPN
                     DLRIDVFRSSGNGGQSVNTTDSAVRVTHIPTGEVVSCQDGKSQLKNKEQALKILKARL
                     YDKALAEQHKDIAAERKSQVGTGDRSERIRTYNFPQGRISDHRINLTLYKLDAFLDGD
                     IDEMIDALITVDQTEKMTAI"
     gene            49516..50196
                     /locus_tag="KRL67_000137"
     CDS             49516..50196
                     /locus_tag="KRL67_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HBH1521051.1"
                     /translation="MILKVTIIGLLAGVIGTGLGGVISAIFKREVDKYLSFFMGLSGG
                     IMLAVVVFDLMKESMDKMGIINTVIFTFVGALITMYIKTKLDVSGNMASGYLIFISIL
                     LHNLPEGLAIGSSFMSTESLGITLAIVIGLHNIPEGLAMALGLVCNKMKLSKVILFTV
                     IAGLPMGLGSFLGVYFGGVFTSLIGVFLATAGGTMMYVVLEEIFPHSKSVYCIIGFLL
                     GTMIVNYI"
     gene            50225..51265
                     /locus_tag="KRL67_000138"
     CDS             50225..51265
                     /locus_tag="KRL67_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonylcarbamoyl-AMP synthase"
                     /protein_id="HBH1521052.1"
                     /translation="MKTIISNIDINNIDYEEIKIQAKLLREGKTVIFPTETVYGLGAN
                     ALDENAVKKIYEAKGRPSDNPLIVHIYEKKEVYDLAKNISDKAKLVIEKLWPGPITII
                     LNKKDIIPYKTSGGLDTVAIRMPSNIIARAIIKEAGIPIAAPSANISGRPSPTKAKHV
                     YEEMNGRVDGIVLGGDSNFGLESTVLDLTEETPMILRPGSITKEVLEELLGEVKLDPS
                     LSKKEDNQKAKAPGMKYKHYSPNADVYIISGKRENVATKINDMIKSNRDKGLKTGVMC
                     ISRNREFYDGEVIDLGNSLEEVASNLFDALIEMDKKGVDVIYSEEFPKTGVGQAIMNR
                     LLKSAGYKVIKA"
     gene            51282..51731
                     /locus_tag="KRL67_000139"
     CDS             51282..51731
                     /locus_tag="KRL67_000139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight protein arginine
                     phosphatase"
                     /protein_id="HBH1521053.1"
                     /translation="MNILIVCTGNTCRSPMAEAILRKAIKESGRSIEEYSISSAGIST
                     ANGMSASENSIEALKEIGIDLSNHKSKVITKKLIDESDIILTMTKSHKEILVQAVPKC
                     KEKVYTFKGFANKNEEDISDPFGGNLDIYRNTMREIMYSVNEIVKKI"
     gene            51748..52200
                     /gene="rpiB"
                     /locus_tag="KRL67_000140"
     CDS             51748..52200
                     /gene="rpiB"
                     /locus_tag="KRL67_000140"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 5-phosphate isomerase B"
                     /protein_id="HBH1521054.1"
                     /translation="MKIGLGCDHGGYNLKKEIISYLEGKGIECVDYGTNNATDSVDYP
                     VYGEIVANSVINKEVDYGILCCGTGIGISLAANKVPGIRCAVVSDVFSAKMSKAHNDA
                     NMLSLGERVLGKGLALEIVEAWINTDFEGDRHARRVNMIKSIEEKHNK"
     gene            52236..52865
                     /gene="upp"
                     /locus_tag="KRL67_000141"
     CDS             52236..52865
                     /gene="upp"
                     /locus_tag="KRL67_000141"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HBH1521055.1"
                     /translation="MSKVVETNHPLIQHKLTLMRDKNTGSKDFRELLTEIAMLMGYEI
                     TKDIPLKDVEIETPIQKTSSKVVAGKKLAIIPILRAGLGMVDGLVSLMPAAKVGHVGL
                     YRDPETLKPVEYYCKLPQDIGERDIIVVDPMLATGGSAVAAIDLLKSKGAKSIKLANL
                     VAAPEGIEEVQKYHDDVDIYVASVDERLNEHGYIIPGLGDAGDRLFGTK"
     gene            53164..53979
                     /locus_tag="KRL67_000142"
     CDS             53164..53979
                     /locus_tag="KRL67_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EF2563 family selenium-dependent molybdenum
                     hydroxylase system protein"
                     /protein_id="HBH1521056.1"
                     /translation="MKNCIVVRGAGDLATAVIHKLHNSGFKVVALEIEEPLAIRRQIS
                     FCEAVYEKEVTVEGVECIFCNTEKEIEEVLSQHKVALIIDPKGEKLSKLNPIVLVDGI
                     LAKKNLGTTKDMAPLTIALGPGFCAGEDVDVVIETMRGHNLGRIITNGRAEKNTGVPG
                     AIKGFSKERVMHSQYDGIMENVCKIGDIVEKGDLLCYINNYEKKHEVRATISGVLRGL
                     LKDGAKVNKNLKILDIDPRKEEVKNAFTISDKARCIAGGVLEAILNNGIYPQN"
     gene            54205..54642
                     /locus_tag="KRL67_000143"
     CDS             54205..54642
                     /locus_tag="KRL67_000143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dCMP deaminase"
                     /protein_id="HBH1521057.1"
                     /translation="MRPSWDEYFMEIAEVVKKRSTCIRRQVGAVIVRDKQILTTGYNG
                     SPRNLEHCENIGCKRQELNIPSGERHELCRALHAEQNAIIQAAHNGISVDGATLYVTT
                     RPCVLCAKMCINAGILKIVYEGDYPDDMSTELLKEAGIELIKF"
     gene            54827..55054
                     /locus_tag="KRL67_000144"
     CDS             54827..55054
                     /locus_tag="KRL67_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AtpZ/AtpI family protein"
                     /protein_id="HBH1521058.1"
                     /translation="MSKKNTHTEIAKLFSLISQIGLMIVISILGCTFLGKFLDSKLNT
                     TPVLTIIFLLLGVGGAFSGVYKTLIVYTRRK"
     gene            55059..55445
                     /locus_tag="KRL67_000145"
     CDS             55059..55445
                     /locus_tag="KRL67_000145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase subunit I"
                     /protein_id="HBH1521059.1"
                     /translation="MDIKLLNQVRSVTKGIVIFDVVLIVLMLITSTLSKPVLLGLIFG
                     SIIAMLNFRLLAISLEKSVTFPAGKAQAYASAQYAIRLFIMAVVLIASATVPHLNIIG
                     TALGLLSTKFVILSKNFINKLKRKEA"
     gene            55448..56152
                     /gene="atpB"
                     /locus_tag="KRL67_000146"
     CDS             55448..56152
                     /gene="atpB"
                     /locus_tag="KRL67_000146"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HBH1521060.1"
                     /translation="MNQAHYYLIGNFKISETILVSWGIMAFLAIASYFLTRNLKKVPT
                     SKVQIFLEFAVGGLAKMVTDVMGKNTVKRMPYMIPYIGSLFLFFACSNLSPLLGLRSP
                     TTDLDTTAAWAMITFFMIYFAGVKFKGLSYFKELAQPLAIMLPINIIGELARPISLSF
                     RPFGNILGGTIIMGLFYQLLGFISSLIPGLSIPIGQILIPVPLHLYFDLFAGLLQAYI
                     FIMLTMVFISSAGELD"
     gene            56221..56481
                     /gene="atpE"
                     /locus_tag="KRL67_000147"
     CDS             56221..56481
                     /gene="atpE"
                     /locus_tag="KRL67_000147"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HBH1521061.1"
                     /translation="METAIVAAASAIGAGIAVATGIGAGIGQGIAAAKAAEAVGNQPE
                     AKGDITSTLLLGVAIAESSAIYGLVISIILLFVNPFFKYLGM"
     gene            56604..57101
                     /gene="atpF"
                     /locus_tag="KRL67_000148"
     CDS             56604..57101
                     /gene="atpF"
                     /locus_tag="KRL67_000148"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HBH1521062.1"
                     /translation="MEKALVGITWEFVFQIVNTFIIFLLLRKLLFKPVLNIIESREND
                     IKSDLAEGEKAKNEGLALKKEYESKINFAKDEGQEIIKQATIRAEQKSDDIVNTAKKD
                     ALDIKEKANKDIEQERQKVINEIKNDISNIALLAASKVIEKDLDKSKHEELIENFIKE
                     VGEAK"
     gene            57098..57643
                     /locus_tag="KRL67_000149"
     CDS             57098..57643
                     /locus_tag="KRL67_000149"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HBH1521063.1"
                     /translation="MINVIANRYAEALFQLGEEENSTDVLFKELEKVVDMMTKVSKDF
                     YKVLKSPLVSKSEKKNLVEIIFSKEVSSNIKNFLKVLVDKDRISYLEDIELAYKELLN
                     KKNNVIDGVAISAIPMSETDIKELEVKLSNKYNKNVTIENVVDKTILGGVLVRIGNEQ
                     IDGTVKTRLDKMKEKLSEVIS"
     gene            57660..59162
                     /locus_tag="KRL67_000150"
     CDS             57660..59162
                     /locus_tag="KRL67_000150"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003405060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HBH1521064.1"
                     /translation="MNLKPEEISSIIKQQIKNYENKVELTDTGSVLTVGDGIASVYGL
                     EKAMSGELLEFPGEIYGMALNLEEEVVGAVILGDDSEIKEGDIVKRTGRIVEVPVGEA
                     LIGRVVNSLGQPIDGKGPIAYTKTRPVESEAPGIIDRRSVYEPLQTGIKSIDSMIPIG
                     RGQRELIIGDRQTGKTSIVIDTILNQKGKDVICIYVAIGQKRSTIAQLVSSLEKGGAL
                     DYTIVVSATASESAPLQYIAPYAGAAMGEEFMYNGKHVLIVYDDLSKQAVAYREMSLL
                     LRRPPGREAYPGDVFYLHSRLLERAAKLSDELGGGSMTALPIIETQAGDVSAYIPTNV
                     ISITDGQIYLQPELFYSGVRPAVDPGISVSRVGGSAQIKAMKKVAGTLKLAYSQYREL
                     AAFSQFGSDLDEDTKKRLAQGERIVEILKQGEHQPIKVENQVMIIYAVINNHLEDIPI
                     DNIARFESELYAFVDNNYPEISRKILGGEDFTHDLTDAINEFKEKFVVEV"
     gene            59190..60068
                     /gene="atpG"
                     /locus_tag="KRL67_000151"
     CDS             59190..60068
                     /gene="atpG"
                     /locus_tag="KRL67_000151"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit gamma"
                     /protein_id="HBH1521065.1"
                     /translation="MAGAGIKAIKTRMGTVESTKQITKAMELVASSKLRKAKEKAEDS
                     REYFNTLYNTVKDIAQTTRGIRNEFLKEREVKNKCYVVVAGDRGLAGGYNSNVLKESV
                     SHMKGKKETVITVGKKAHEFFSKRNYNIVKDINSVEACGYEDATEISQTIIDLYKKGE
                     VDEVYIAYTKFKSALSQEVEILKLLPLVFEKDTDEQGMAVKEEKQNRSKVQYIPSAEA
                     VLSYIVPQFISGTVYGGILESFASEQGARRTAMESATDNANEMLSNLELSYNRARQSA
                     VTQEITEIVGGVEALK"
     gene            60081..61475
                     /gene="atpD"
                     /locus_tag="KRL67_000152"
     CDS             60081..61475
                     /gene="atpD"
                     /locus_tag="KRL67_000152"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HBH1521066.1"
                     /translation="MANVGKVVQIVGAVLDVKFDSEQSLPNLLNALVIKLGDKEIVAE
                     VAQHIGDDTVRCIAMSATDGLVRGMEVVDTGGPISVPVGDETLGRIFNVLGKPVDGKP
                     APKSAPKLPIHRPAPAYDELETTAEILETGIKVVDLLAPYLKGGKIGLFGGAGVGKTV
                     LIQELINNIAKQHGGISVFSGVGERTREGNDLYGEMSESGVINKTALVFGQMNEPPGA
                     RMRVALTGLTMAEHFRDEQGQDVLLFVDNIFRFTQAGSEVSALLGRMPSAVGYQPTLA
                     TEMGALQERITSTKKGSITSVQAVYVPADDLTDPAPATTFSHLDAKTVLSRQISSLGI
                     YPAVDPLESTSRILDPSIVGKEHYEVARGVQSILQRYKELQDIIAILGMDELSDEDKL
                     IVARARKIQRFLSQSFTVAEQFTGNPGQYVPVKETVRGFKEILEGKHDDLPESAFLFV
                     GTIEDAVRKAKGSM"
     gene            61478..61738
                     /gene="atpC"
                     /locus_tag="KRL67_000153"
     CDS             61478..61738
                     /gene="atpC"
                     /locus_tag="KRL67_000153"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit epsilon"
                     /protein_id="HBH1521067.1"
                     /translation="MASEFSLKVVTPSNTFYDGDVEMIVVRTTKGDKGVLKGHIPFVS
                     GIPVGTLKIKKEGSFKEAKIAEGFINVTQDKTVILTESAEWI"
BASE COUNT        25092 a         6614 c        11492 g        18804 t
ORIGIN      
        1 attattgagc gattattaaa aagatatgta taaattttaa tttgggaact tgtattatat
       61 atcatgaaaa aagagaaaaa ataaaaagta aattgtttct tgataacttg aaattggaag
      121 tgagaatatt taactagaga agaattatta taagttcagg ttagctctag gagtagctga
      181 ttttatagga acaatagatt ttatggatga tgatataaac atgatagcaa ttagaccaga
      241 agatataaag gtagaattta atagagattt caaagaagtt gatataaaag tacgagagat
      301 tttggatatt gaatttagaa gattcaacta tatagttaca gtttaatatc gttctaagca
      361 aataaagttg aatgtagttt caaatgtagt ggaacaaatg gaactatgtg taggttataa
      421 aataaacttc aagataccta aagaaagaat catataatat aagtaatcca ttaatttttt
      481 tatagacctc aaactcagac cgcatcgata taaaaactaa agctagatga tatagtgttg
      541 tatataggac catcagctgt tatggcagta ttagtgcttt taacaaatat aatagtaaaa
      601 cttttgtatg aattgataat aaaaaaatta aaaaccaaga cataaaaatg gcaacaaaaa
      661 gaagttgaat aagggaggta aaaatgaaat ttaagaagtt agtctctttg ggaatattat
      721 caacactttt attgagtgta ataactggat gtagtaatgt ttcatcaggt tcaaacttaa
      781 agtctaataa taagacaaaa ggagagttaa caatttatac agcaattgaa gaagatagta
      841 tagatacata tttaactaca tttaaagaga aatatccaga tattaaattg aatatactta
      901 gattatcagt aggaaatata acagcaaggt tattagcaga aaaagataat cctaaagcag
      961 atgtgttatg gggggttgca gctacaagtt tgataatttt agatgacaaa gaaatgctag
     1021 agccatacgc tccgaaaggc tttgataagg taaatcctgt atttaaagat gataagaaag
     1081 ttccttcttg ggttggaata gatgcttgga tgactggtat aacagtaaat actaaagaat
     1141 taaaagctaa aaatatatca actcctagtt cttacgaaga tttaataaag ccagaatata
     1201 aagatcttat atctatgcct agtccggctt cttcaggaac aggatattta acagtatcgg
     1261 aactcataca aattatgggt gaagaaaaag catggcagta catggataaa ttacataaaa
     1321 atattggtgt atacactcag tctggttcag ctccagctat attagcagct tcaggagaat
     1381 atcctattgg tatatctttt ggaaatagag gtataaagtt aaaaggtgaa ggttatccag
     1441 tagaagtggt atttccaaaa gaaggttctg gatgggagat agaatcaaat gcacttgtga
     1501 aaaaagataa tataaaagaa gcatcaaaag tatttttaga ttgggctata agtaaagatg
     1561 caatgaatga gtattctaag aattttgcag ttactacaat tgacacggga aatccagtgc
     1621 cagaaggatt tccaaagaat cccttagaac agatgataga taatgattta aaatcagcag
     1681 caagaaatag agaagctata ctaaataaat ggatagaaaa atatgatgga aagacagaaa
     1741 aagagagcta agctttacaa taaaatttag tagaaaaagc gcctagaata taaattttat
     1801 atctaggctc ttttgatata tttatttaaa atgcatattt ctatataaaa ataaatagtt
     1861 tagtaccttg attaaattaa ataaaaacct taaaataaag agatgtaaag aaaaactaaa
     1921 aacgaataaa aggtgaattt atatgataaa agaaataata gtagtagagg gtagagatga
     1981 cgtcacagca gttaagagag caatagatgc agaacttata actacaggtg gatttggatt
     2041 tccaaaaggt gtaatggagc gtataaaagc tgctaatgaa agacgtggtg ttataatatt
     2101 tacagaccca gattttgcag gtgaaaaaat aagaaaaaaa atagcatcag aagttccagg
     2161 atgtaaacat gcatttctac caagagagga agctaaaaag gatggagata taggtattga
     2221 gaatgctact cctaaaagta taatagcagc tttgaataaa gttagaactg aaaatacaga
     2281 aaaaagagat gaatttaagc aagttgactt aattagaaat ggtcttatag gtaatgaaga
     2341 agcatcactt agaagagatg cacttggaaa aatacttgga attggatatg gaaatgcaaa
     2401 acaattttta aatagattga ataattatgg agttgaaaga gatgaattta tagatgcatt
     2461 aaaacaaatc taaacgtaat gtttaatgta tgaaaaatat gtatataaaa taaattgtgg
     2521 aattacggtt aatttaagat aaattattta taatatatac cttattttaa atcaatgtaa
     2581 atacatataa ataagtataa ttaataaaat taaatatcaa aataagttaa aaaggagtaa
     2641 ataatggata gactatcttc acataatgca acaaaggaag tagtacaaaa atataatttt
     2701 aaattttcaa agtctttagg tcaaaatttc ttgatagata gtaatataat tgataaaatt
     2761 ttatcgggag ctagaatcac aagaggagat aatatcatag aagtaggacc tggtatagga
     2821 actcttactc gtgagatggg taaaatagca gaaaaagtag tggctataga aatcgataga
     2881 aatttaattc ctatattgaa agataccttg tcagatttag ataatacaga ggttgtaaat
     2941 caagatatac taaaagtaga catacaggaa ttagtaaaag ataaattaaa tggtggacca
     3001 gttaagcttg tagcaaatct tccatattat ataactactc caatagttat gaaattttta
     3061 gaggaagata tacctgtaac agatatagta gtcatggttc aaaaagaggt tgcagataga
     3121 atgaatgcta ttccaggtac taaagattat ggggcattat ctatagctgt tcaatactat
     3181 tgcgagacag agattgtggc aaaagcgcca agacatatgt ttataccaca accaaatgta
     3241 gattccacag ttataggtct acatgtaaga gataaaagaa aatatgatgt tcataatgaa
     3301 gaaatatttt ttaaaacagt aaaagcatcc tttggacaaa gaagaaaaac tcttcttaat
     3361 tcgttgggag gtcttggttt tcttaataaa gatgaaataa gagaaatatt aaaagaagct
     3421 aatatagacg aaaaaagaag gggtgaaact cttagtatag aagagttttc agttctttca
     3481 aatataataa atacaaaagt atcttctaaa tagaaggtac ttttttttat tttgttaaca
     3541 tatattgttt agagaaactg tgttaagggg ggattgcagt gggttctaaa agaaagttta
     3601 aaagagacta tataatacta gaagctaaag acataaactt tagatacaaa gaacgagtac
     3661 tgccaaaagc ttttgcaaaa gttgaagtaa atgatgaaaa agcaattatt gcattatatg
     3721 tagaaaatct aaaatatgtt aaagatgggt ataaagcagt agcaataaaa gaagaccatg
     3781 gattaataga cttaggaaat ataattttaa atgaacaagg aagaggtgaa tttgttttag
     3841 atttagaaga ttatgatgat gagataaaag gtattgcatt attgtatgaa agaaatatac
     3901 ctttaatcgg atttaaggga aataagcttg aaaattatga ggacatatta tttcaagagg
     3961 tagaggaaga agaatatgaa gagttggaag aagaagacga agagatagag gataatgagg
     4021 aaagtgatag agacgaagat agtgaagaag aagaatatat tatagaagaa tatgatgaag
     4081 atgagtatga agactattgg gaagatgatg aagatattga agaaggagag ttaaaggcaa
     4141 gaacggaagt agaagattct ggtgatgagg aaatagaaga gttcaataat tataaggaaa
     4201 tagaaaatta taatagggtt aaagtacagg aagaatatat agacatagaa tatgatgagg
     4261 aagaagagga tgaagaagaa gtttttgaag taaatagaga agaagatagg tttgtttcct
     4321 caaaacctac taaatcaaag aaagttcaaa aagatcagaa taatattgaa aaaataaaaa
     4381 gagctaatgg agtaagtcaa ggagagagtg gaaataaaca aacaagtaca ttacttatgc
     4441 caagacaaat aaaaaaaggt ttgaaattat ttaaagaagt aaaacctttt gtaacagatt
     4501 atatagataa gacaagatgg tggaaaatag agataaatcc tacaactttg tgtggatata
     4561 caatgcctta tttaggatat ttaaacgtat tgaattacac catgtatagt gatgcagttt
     4621 tacattcata taaatacaga cactatttat ttggagttca gtatgatgaa tataataaga
     4681 gaaaacatta tatttatgct atcccaggaa atagaaatga acagccagac aatggaagca
     4741 caggattttt aagatatcaa gcttgtgata atagaacaaa tagtttagga tattggttat
     4801 gttttataga ttctagacaa agaaatatag ttagataaaa ataataaaaa tgaaaaaatt
     4861 tagactcaat tcttatttta ctgaaaaaat taatgaaatg tagtacaaaa ctttaaaaac
     4921 tattgataat atttaaatat tacactacaa tatatcttga aatacctatt atattatgga
     4981 ggaaaattat gataaacgag caaagaattt taaatgagtt tcttgaatta gtacagatag
     5041 atagtttatc tttaaaagag gggaatgttg ctaaagtatt agttaaaaaa ttagaagaaa
     5101 taggttgttc agtattaata gacaatgctg gtgaaaaagc aaatggagaa actggtaata
     5161 taatagctac attaaaggga aataaagaag gaaagaaaat attatttagt tcacatatgg
     5221 atacagtaac tccaggtata ggggtaaaac ctatagtgga tgaagcaaat ggaataataa
     5281 aaagtgatgg tactacaata ttaggttctg atgataaagc aggtatagca gctatattag
     5341 aaggcttaag atatataaat gaaaataaca tagaacatac tgatatacaa gttgtattct
     5401 ctatatgtga ggaatgtgga ttagttggag ctaaaaattt agactatgga aaaatagatt
     5461 ctgaatatgc gtttatatta gatagtggtg gttctccagg tgaaataata gtaaaagctc
     5521 ctgcacaaga tgtaataaat gttaagatat taggaaagac tgctcatgca ggtcttgaac
     5581 ctgaagctgg aataagtgct ataatggtag ctgccagagc aatagaaaat atgaatttac
     5641 ttagaataga cgaagaaaca actgctaaca taggaattat aaacggtgga acagctacaa
     5701 atatagtaac aggagaagtt aatatagttg cagaagccag aagtttaaaa gaaaataaat
     5761 tagatgttca aactaaacac atggtagaga cttttgaaaa agctgcaaaa gattttggag
     5821 ctcaaattga gatagatgtc aatagagcat atacaccaat agatgttagt gaagattctg
     5881 aaataataaa acttgcaaag aaggcttttt caaacttagg tatagaaggt catactgaat
     5941 ctacaggagg aggtagtgat acaaatatat taagtaagaa tggtatcgaa gctataactt
     6001 taggaatcgg tatgaaaaat gctcatacac taagtgaaca tatagctata aaagacttat
     6061 acgattctgc taaaatggta gtggagataa taaaggaagc ataaatttaa ataatagtgt
     6121 ataatatata tatcgcctag cttaagctag gtgatttttc attttataga agattgataa
     6181 tctctattga aagtaaagag tatacatttg catataagag agagtttaat ttatgttcag
     6241 atgtaggttc ttaataatat ttagattgtt tcataaggga ggaataaata tggaaacaag
     6301 gtatgaagaa gcaaataaaa taacaataca atcaatactt tggaatgtag tattaacaat
     6361 aattaaggtt atagctggtg ttattggtaa ttctagcgct atgattgcgg atggattaca
     6421 ttcagcatca gatattataa gttctatagg agtactaata ggcaattatg tatcttctag
     6481 acctggagat agagagcata attatggtca tgagaaagca gagacattag tatcatttgt
     6541 gttatcaata ttgcttattt tcgtttctat aacaataggt atagaggcaa ttaaatcttt
     6601 atttaactta gatgcattaa gtgttccatc aatattacca ttagtagtat cagttatttc
     6661 aatcctgata aaagagtatc aatatagaat aacaataaaa gttgctaaaa agataaattc
     6721 accagcccta aaagctgatg cttggcatca taggtcagat gctttatctt cagtagctgc
     6781 ttttataggt ataggaggtt ctattttagg atttaagcca ttagacccaa ttgcatcagt
     6841 tgtagtggct atatttgtag ctaaagtagg aattagtata cttataagtt ctgttaatga
     6901 attgatggat gtttcagttg atgaagaaga gataaaagaa ttaaaattta ttgtagcaga
     6961 tacagaaggt gttaaaaatc ttggtgatat aaagactaga aaacatggag ctatggcata
     7021 tgtagatttg actatatgtg tggatgaaaa tttgacagtt aagcaaggtc atgatattgc
     7081 tactaagctt gaaaaacata taataaagca tatggaattt gtaaaaggca taacagttca
     7141 cgttgaacca tgtactaatt gccaaggaaa taagtgtaat aattaattaa aacttaaaaa
     7201 aaagtgtaaa aagttatata attgttttat gattataaac tctaggggtg gttaaatgag
     7261 caagaaaaga aaactaaaga aaaaagctgc aatattaata ggagtagttg ttttctttat
     7321 aatatacttt atatccttta gaataggaat aaatatggaa aaggataaaa gtgaagtgcc
     7381 aaagaaggta gaaaaagaag aagaagataa taggtcttta aggactcaaa taaaagaaaa
     7441 gaagaaggtt tatatatcag acaaaaatat agaaaacata aaaattgaag aagatatgtg
     7501 ggatgaaata aagttatttt tctcagattt taagaaagta aggaatacta cttcttctta
     7561 cgaagcagtt tatgaaggtt attctgatga tggagttaaa ttttctactg accttaactt
     7621 ttttagagta tatactgtaa ataaagaaga atattataaa gtaccagtta gttctaagga
     7681 tgcttttaag aaacttttag atgagagtat atatacttcg tttgaccttt taagtcatta
     7741 taaagattgg aaagaggtta ctataactca tggaaaagaa attaaaaaag ttcacagatg
     7801 gaaatttgat gatttagcac ataagatgat ttcaaaaagg gttgtaggaa aagttcaacc
     7861 tgagaaaagt aaggaaagaa gtgactataa ttttactata aatataaaag gtgataattt
     7921 tgatgtaaaa gttgatacaa tgggaaaaga ttatgtaaaa gttacttcta agaaaaatgt
     7981 atcttactat gaggttcata caagtttatt tgattacctt aaaaatgaga tatttgaaat
     8041 tggaaagaaa taagacttta agaaggctat caaattagat atttgatagc ctaaattatt
     8101 ttatatatta tttttcaacc aacaagtctg caaccttata aattggacca gcaccagcag
     8161 ttattactaa atcattgtcc ttaacatttt cacgtaagta ttttactata tcttcaaatt
     8221 cttttatata gatagcatct acattgtttt gatataattt ctcaaccaag tccttagaat
     8281 gtatatctcc tgggtctttt tccctagcag catatatatc cgttatgata actttatcag
     8341 cagcatagaa tgcctctgaa aattcaccta aaagagattt tgttctagta taagtatgtg
     8401 gctggaagat acaccataaa gtggattttt ttaatttttt ggcagcagct aaagtagctt
     8461 ttaactcagt tggatgatgt gcataatcat ctactactaa agcattctta taataccctt
     8521 tagtttcaaa tcttcttccg acacctttat aaatttttat attttttctt atagtctcta
     8581 agtcaatacc agaaacaaga gctgccatta tagcagaaga agcattgtat atgttatgaa
     8641 gaccatatac agaaagttca aattctccta aatctcttcc ttcatattct attctaaata
     8701 ttccatgacc actattgtca aagtgaatat cttttattac agcatcattg tcagaatctc
     8761 taccatattt tataatagta gctttaacat catacaatat gtcatcaata ttttcatcat
     8821 cgccatttat tatgaaatag ccttctgggg gtaataattt tccaaatttg ttaaaagaag
     8881 ccttgatttc atctattcca gagaaataat caagatggtc ttcttcaaca tttaaaacta
     8941 tagatatttt tgggttaaaa ttaaggaaac tatctacata ttcgcatgct tcagttataa
     9001 agtggttgga attacctatc ttaacattac cacctatcat acttaagtta ccaccaacta
     9061 gtatagttgg gtctaagtct gcatattcaa agattgtaga taacatagat gttgtagaag
     9121 tttttccatg tgttccagaa actgctatag aatttttata ttccctcatg atttggccta
     9181 aaaaagtcgc tctgttcatt accaatttat ttttttcttt agcagccaca agttcttcat
     9241 tgtctggatg gactgctgct gtataaacaa ccatatttac atcatctgat atattatctt
     9301 ttttctgacc aatataaata gttgcccctt ggtccctaag tttgtctaat aggtatgatt
     9361 cgtttgaatc cgaacctgaa acttgataac ctttattgat acatatttca gctaatgcac
     9421 tcatgctgat accgccaatt cctataaagt gtatattcat aataaaacca cctttctttt
     9481 taattaagtc ctacaatatg aactatttct gtggtataat aatataatgt tcaatatttt
     9541 ataattaata tgcatataat attatatgta ttttattttt gaatataata aaacataagt
     9601 aatattatac cacaataaga attgttatgc attagttagt ttaacgttat ataaaggtac
     9661 ttgtaggagg aaataataat gaaatttaaa agaacagaga gaattggagc aatagttaaa
     9721 atactgtctg ataatccaaa taaaatatat actttaagtt attttacaaa tcaatttaat
     9781 gcagcaaaat caacaataag tgaagattta ttagttgtga aaaatgtatt tgagaagcta
     9841 catttaggta aagttataac tatctcagga gcagcaggag gagtaaaata tatacctaaa
     9901 acttctatag cagaaaatgc agagttctta atggaattat gtgagagaat atgtgacaag
     9961 tctagaatac tatctggtgg ttttttatac ttgattgacc taatttacga ccctacaata
    10021 gctgctaaga taggaaagat attcgcatca aatattgagt atgtagatgc agattatgtt
    10081 gtaacaatgg aaacaaaagg tatacctatg gcacttatga ctgcaaaagc tatgaattta
    10141 cctctagtta taataagaaa agatataaag gtatcagaag gtcctacact tagcatgaca
    10201 tatgtgagtg gaaacagctc taaagttgaa agtatgagtt tgccaagaaa agctcttaaa
    10261 ccagatagta aagttataat tatagatgat ttcatgaggg gtggaggtac tataaaagga
    10321 atggtagacc ttatgaatga atttggtgca gaggttatag gtactggagt attcatatct
    10381 acaacgaatc cttcagaaaa aatggtaaaa gattatattt cattgattca attggatgtt
    10441 aatggagata aaatagtagt agaaccaaac ttaaaaactt ttaaagatga atatagaaat
    10501 gaagatttag acgaagaaga agattcagaa tttgaatttg agatagatga agattaataa
    10561 aaaaaatatt tagaaaaata tgtaaaaata aaaagaggat atccctagtt gtccatagaa
    10621 ttatttaaaa aacagtttat attaatttat tccagcgata ataattttaa tataaattaa
    10681 tttctgtcaa aatcaaaggg ggatattttg gtatgaagat aactgacgta agagtaagaa
    10741 aattaacaga agaaggtaag atgaaatgta tagtttcaat aactttcgat aatttgtttg
    10801 tagtgcatga tataaaagtt atagaaggcc ataatgggct atttatagca atgcctagca
    10861 gaaaagtagg cgaaggaaat ttcagagata tagctcatcc aataaatgca gaaatgagac
    10921 aagttttaga agatgcagtt ttacaagcat atcatgaagc attagttcaa tgggaagtag
    10981 ctgctgaata ataataaata tataaaatgg agagagcccg aacatttagg ctctttttaa
    11041 atgtagaatt tctattaaaa tatgtacttt ttagcaaaaa agtgctatat tattagtgat
    11101 aatgtatatg ttgtatacaa attgttagat aaatgtatta taatgtatat tgattaagag
    11161 aataatacga ataaaattaa attagattga atggagtgta tcaatgaact ttaaagctat
    11221 aatccttgct gctggcaaag gaacaagaat gaagtctaaa tacccaaaag ttatacataa
    11281 agtatgtgga aaagaaatgg ttaaccatat tatagatgtt tcaaaaaaat caggggtaaa
    11341 agatactgtc gtgattttgg ggcatgaagc tgatgtagtt aaagaaaagc ttgcagaaga
    11401 aattataata gccatgcaaa cagaacaact tgggacagga catgctgtaa agatggctaa
    11461 agaatatatt aatgatgaag atacaatagt tgtactttgt ggagatactc cacttataaa
    11521 agaagagaca ctaaagagat tatttgaata tcatatagaa aataaatatc atgctacagt
    11581 tcttacaaca agagttggaa atccaacagg ttatggtaga ataataagag ataagaaggg
    11641 tgaccttctt aaaatagtgg aacaaaaaga tgcaaattca gaagaaaaga tgataagtga
    11701 gataaactct ggaatttatt gttttaatgg aaagagtctt agagaagcac tagatttgct
    11761 aaataataac aattcacaag gtgaatatta tttaactgat actgctaaaa taatgagaga
    11821 taaaggatta aaagttggtg catttgcagg ttcaactatt gaagaactta tgggcgttaa
    11881 ttcaagagta gaattatcaa aggcagaaga aattatgaga cgaagaataa atgaatctca
    11941 tatggtaaat ggagtaacta taatagacac taactctacc tatatagaat ctgatgttat
    12001 gataggcaat gatactataa tatatccagg tgttatgtta caaggaaaaa caaggatagg
    12061 ctcagattgt ataattggaa tgaattcatc tataacaaat tcagaaatag gtgatggtac
    12121 agaaataaaa aattcaacta taattgatag taaggttgga gaaaattcta ctgttgggcc
    12181 atatgcatat ttaagaccaa agagtgactt aggaaataat gtaaaaatag gtgattttgt
    12241 agaagtaaaa aatgctatta tagaagatgg ctcaaaagca tctcaccttt cttatatagg
    12301 agatgcacat gtaggtaaaa atgtaaatat tggatgtgga gtagtatttg taaactatga
    12361 tggtaaaaat aaatttaaat cagtagtaaa agacaatgcg tttataggtt caaattctaa
    12421 tttagttgca ccagtagttg tagaagaaaa aggatatata gcaacaggtt caacaataac
    12481 acatgatgtc ccagatggtg ctttggcaat agcaagagaa agacaagtta tcaaagaagg
    12541 atgggtagaa aaaaagaatc aaaaagacga ccaaagtaag taattatgta gtttattatt
    12601 ttggctaaaa aatttagctc gaaattatta taaattatat tcattagtat tttaaaattt
    12661 tcaggagggt tacataaatg aatactagcg gaagcgagat taaaattatt gcaggtaatt
    12721 catcgaaaga gttagcacaa aaaatagctg actatatagg tgtatcagta ctagattgtg
    12781 aagtaggtac atttagtgac ggtgaaatat gtgtaaacat gaatgagaca gtaagaggtt
    12841 gtgatgtgtt tgtagtgcaa tctactaaca gcccagtaaa tgataactta atggagttat
    12901 taatcttaat tgatgcgtta aagagagcat cagcaggtag aataacagca gttattcctt
    12961 actatggata tgcaagacaa gacagaaagg ctaaggcaag agatccaatc actgctaaat
    13021 tagttgcaaa tttaataact gctgctggag cagatagagt attaacaatg gatttacatg
    13081 cagctcaaat acaaggatat tttgatatac cattagacca cttactaggt ggaacaatat
    13141 tagcaaatta ttttaatgaa aagaaaatag aagatttagt agtagtatct cctgacttag
    13201 gaagtgttac tagatcaaga aaatttgcta atactttaaa tggagaagtt ccaatagcta
    13261 taatagataa aagaagaccg aaagctaatg tatgcgaagt tatgaactta ataggtgatg
    13321 ttaaaggtaa aaatgttata ctattagacg atatgataga tacagcagga acaatagtta
    13381 atgctgcaaa tgcacttaaa gagtttggag caaaagatgt ttatgcttgt tgtactcatg
    13441 gtgtgttatc aggtccagct attgaaagaa ttgctaattc agaaataagt gagttaatag
    13501 ttcttgatac tatacaactt cctgaagaga aaagaataga taagataaag ataaaaacag
    13561 tagctccttt atttggagat gctataagaa tgatattctc taatgaatca gtaagtaaat
    13621 tattctaatt tattgtattt aggagagttg tttaaaagga tgtaaacctt ttgagcaact
    13681 cttttttgat tttatactta tgaaatgcta tttttctctt gttagaaagg tatatattat
    13741 tgtagaaaga aattatagtt taaaattatg tatatttaag caaaataaac ttaataaagg
    13801 gcatattatt cataatattg tgaatataaa aaatattaca aaaaagtatt gaaaaaattc
    13861 atattatgta gtattatgta aatataaata aagaaagagg tgtttccaat gggaaggtta
    13921 gaagatgaac ccctaataat taaatgattc agtcacaatc taaagtgaat ataaaaatct
    13981 attgaaagtt tatttataag aattgaaaat agtatctatt ctatctttag tgtttgtgag
    14041 agggttctgg attatataag attactgtaa agaagtaatt ttgaacaata agcatatctg
    14101 taaatgaaga gtttcgttta tataatgatt gtggctgaat cataactata atagatagaa
    14161 tgtttataat agatgatttt ataaaatact atattgtaat agcttttagc tttcaaataa
    14221 atattagcaa aggatggaaa tgacaatgat aaaactaaat gataatttat aaatatctcc
    14281 ttacgatata taataattgt agggagatgt ttcttttttt gtatagtata attttttcta
    14341 gaaattctgt ttcaatatgt aaaaagttgg tatatataaa tagtataaac tgtgttgcta
    14401 aaatatggta gatattatat aatataagtg gatatgtata tgcattatga gttatataat
    14461 tttgaattta ataaaataga aacgttgatt tatgttctgc aatgtgggca ccttggagca
    14521 tattgagtta gtggtgcaac cggctatgaa attgtattta tttatagata ctattatttt
    14581 catagctttt tttatttaaa aaatggttat atgttagaaa taaatttggt tagtacgttt
    14641 aaataatttt acttagattt ttaaccagag atattataag taagtattaa ttaaaaaatt
    14701 taagggggaa taaaaatgaa gttaaaaaag aataaaaaag gtttcacttt agtggaatta
    14761 ttggtagtaa ttgcaattat aggtatatta gcagtagtgg cagttccagc tttatttagt
    14821 aatataaaca aggctaaggt agcaagtgtt gagtctgatt atagttcagt taagagtgca
    14881 gcattatctt attattcaga tactaataaa ataccagtta caccagatgg tcaaactggt
    14941 ttaaatgttt tagagactta tatggaatct cttcctgata aagctgatat aggtggaaaa
    15001 tataaattga ttaaagttgg taataaatta gtattacaga taggtaaaga tggtgaagga
    15061 gttaccttaa cagaagcgca atcagcaaaa ttattgagtg atataggtaa agataaaata
    15121 tatacaggtg ttacaggaga taattttgga gaccaattaa aagatactac aaaaatagat
    15181 aataaagctc tatatatagt acttatagat aatactgtga tggactcaac aaaataatta
    15241 aaactatatt tttataaaga atattgtaaa tttaatgaaa aatataatat aaaagacagg
    15301 aagaaaatct tcctgttttt ttatactatt ttctttaatg ttcaattatg aatgatgata
    15361 taattgagat agtaaaggag tgaaacctgt ggctaaaaaa gttagaatag gagacaaact
    15421 ggtcgaaaag ggatatataa cagaagacca gcttaaatgg gcactatcag aacaaaaaaa
    15481 cagtggaaaa agactaggag aatttctagt acaagaaggg cttatagata gtaacttact
    15541 aataagtgta ttaaaagaac tcttggatat agaaagtata tttttagaag ggacagaaat
    15601 agacacttta gctacaaaga tggttccaga aaatatatgc aaaagatata ctgtatttcc
    15661 atttaaaatt gatggaaata aaatatgtct agccatgtca gacccacaag atagagaagc
    15721 agttcaagat gtaaggcgta tgtctggtaa agacgtagag atatttatat caagtacaga
    15781 agatataaac aaagcaatag gtcatgctta tgcacattca gaaataaata aggcaatgac
    15841 tgagtacaac aaaaatagaa ctggtggagt aagagaaaca gtaatacttg aagaagatgt
    15901 aaatgctgct ccaatagtac gattagtaaa taacatacta gaaaatgctg ttagaatgga
    15961 agcaagtgat atacatatag aacaaagtga aaattacatg cgtgttaggt ttaggataga
    16021 tggaatgtta agagaatata tgaggatgaa ctcagcacca tataaggcag taatcagccg
    16081 tatcaagata atgtcagata taaatatatc tgaaaagaga ataccacagg atggtagaat
    16141 ctatttaaaa gtagataata aacccataga ttttagggtt tctacaatgc caactaacag
    16201 ggatgaaaag atagctatga gggttcttga taagagtaat tttatggtta gtaaagaagt
    16261 actaggaata gacgaacatg gctctaagat atatgatgaa ttgataaaca ctccttatgg
    16321 tcttatattg gttgttggcc ctactggaag tgggaaaacc actacattgt attcaatgtt
    16381 aaatcaacta aacacagaaa atagaaatct attaacaata gaagacccta tagagtatga
    16441 attacctggt gtaaatcaat ctcaaataaa tgaaaaggca gggcttactt tcgccagcgg
    16501 gcttagagct tttatgcgtc aagacccaga tataataatg gttggagaga taagagatac
    16561 tgagacagca gaaatagcaa ttagagcttc tcttacagga catttagttt taagtacatt
    16621 acatgcaaat acagcagttg gagcaatttc acgtctttta gatatggatg tggaaagttt
    16681 tttaataact tcatctgttt taggtgtaat atctcaaaga cttacacgaa aaatatgtga
    16741 gcactgtaag gtttcatatg aagcagatat tggagaaaaa aaggctcttg gtattggtgt
    16801 aaatgaatca ataactattt atagaggaaa aggttgtgaa aggtgtaata atactggata
    16861 taaaggacgt ctaggtatat ttgaaatgct tgaaattact ccagaaataa aagagttaat
    16921 agattcatct gcaaatcaaa gagaaatttt aaagatggct agaaaacaag gtatggttag
    16981 cttaaaagag gatatagtta aaaaagtttt aaatggaaag acaacagtag aagaaatgat
    17041 tagaataatt ttgatgactg attaaaggat gatattatga aaagttttaa atacacagta
    17101 ataaaagagg acggaaaaaa gcaaactgat gtgattgaag ccaacgattt taatgaagct
    17161 agaaatcttc ttagaaagag aaatcttaga gttattgaaa tcaaagaaag caaaaataaa
    17221 aatataagat tatctagtag aaagagaaaa aaagacttgg gagctgacca gataagccac
    17281 ttttgcagac agtttgctat tatagtatct tctggtataa atagtatttc tggattagaa
    17341 acattagcta gaaggtcaac caacattaca ttaagagaag aaattaatcg tatagtggca
    17401 gaaataaaag taggttctac tattgcagat tcaatgcttt catcaaaatc aaagtttcca
    17461 aagttactcg gtgctatggt agctacaggt gaagctacag gtaaattaga tgaagtatta
    17521 aaaagtatgg cttcatttta tgcaagtgaa cacagagtta aacaaaagct tagaaatgct
    17581 gcaacatatc cattaatagt tttgatttct tcatttataa tgatttttat atttacaaca
    17641 tttatggtac ctaagatgat taattcaatt acaacagtag gagcaactct tccacttata
    17701 acaagaattg ttatggggtt tggtatgttt atgaagaagt tttggataat agtactttta
    17761 gttattatat tatttatata tcagtttaaa aaatatctaa aaacgccagt tggaagggct
    17821 cataaagata gagttataaa caagatacct gttttaggaa aaggaataaa ttgtatggta
    17881 gctacacgat tttcaagagc attgtatcta tttgtatcaa caggataccc attagttcaa
    17941 gggcttgatt atattataga tagtgtaaat aacaccatgg cagaaaagat tttagtatca
    18001 gctaaagatg gaataacaag aggagaaggt ttagcagaaa atttagaaag atatacgtat
    18061 tttgactctg tattggtaca gatgatagct ataggggagc agacagggga attagagaat
    18121 ataagtagac agatggccga attttatgag tatgaatccg agatttattt aaataggatg
    18181 gcctcaatga tagagcctgt tttaataatt gcagttggta ttatagttgc gatactggtt
    18241 gtatcaatat ttatgccaat gcttagtata tatgatgcta tgtaggaggg aaaggacaaa
    18301 tgaacaaggt ttatgtatca tattatggcg attctatttc catagttgag ggcagatata
    18361 aacacgataa ggataagttt tatgtaaaaa attttaatgt gttttcaata gaggatattg
    18421 cacaagattt ttctaaggaa aagtatgctc tattgagata cgcattagaa aatgttaagt
    18481 taaaatcaag acatgtagtt ttttgtttaa acacaagaga tgtaatttta aaaccccaaa
    18541 aacttcctaa tatggataaa aaggatttag acggatttat gaagctagag atggacgaaa
    18601 tgatggcttt ggagtcagat gaatacgtat tttcgtatga agtatctagt gaaatacctg
    18661 ataattcaga tggcgatgaa tcaagcttaa atcttattat tggagctata caaaaggaag
    18721 aggtaaatgc aattgtagag ataatgcatg aatttgattt aatattagat agaatagata
    18781 cattatcaac agcttactta agaatgctta aaaatttaga ttacaatgat ataatggtaa
    18841 ccaatataag tgacaatagc acaattataa atatatataa gaaagatact ttatttatta
    18901 gtgataatat acctataaag ataactgcta aagatatgga catgcaatta ttaaatgtag
    18961 tagaagaatc aaaaggtctt atgaattact attcgtcaag aaactttggt aaggttacgg
    19021 atactatatt aatattagga aaaaggtatt ccaataaatt aatttatgaa acttttagct
    19081 ctgcattttc aagctatgtt tctcaaggtc ttactgagat aatggatata tctgatatga
    19141 taactggaga tatagatgaa gatgatataa attcaattgt agagctttta ggaagtatga
    19201 tagaacctaa agggaaaaga gattttgaaa atataaactt tttaccaatg gatatactta
    19261 gaaaacaact aaaagctaaa cagatgaaaa acatactgaa aattgctcca atagttattt
    19321 tgattcttgc tataccttat ttatgtttaa ttttttcaaa taatttagca aataaagaac
    19381 ttgaaaatgt taatggtgaa ataagaaata ttgaatctgc atattatcag attgggaata
    19441 tagaaaaaat gatacagagt aaaagcgaag aaataaaggt atataatatg ttaattggaa
    19501 aaaaggtaaa gtgggaaaat ttattggatg atatagataa aaatactcca agtaaggttg
    19561 aactaacaag tttatcaacg acttatcaaa atcttggaac taataataaa gctgataatt
    19621 caaaaagtaa taagactaca gcagataaat caagtaacaa tgaaataaaa tcaaatacga
    19681 aaagtgatag taatggagat aaaacaaata atgatttaaa tgaagataag actacaaatg
    19741 tgtcagaagc attaactgga aatagtgaga ctaatagtaa taacacagat aaagatgata
    19801 aaactccatt gtatgataag ataccaaatg ttataaatat agaaggaaat gctaaagact
    19861 cctcttatgt aggtcaattt ttatataaat taaagggatt gtactatttc gaggatgtta
    19921 aattacatag cataaaaagt aatgcagaag attctaacta tacatttagt atgacattat
    19981 acctgaaaga aggtgtatta actaatgaat aaagatatat ttaaaaaaga tataaaagat
    20041 atatttaaaa tggaagttag tctaaaaggg attcttataa aggttataat tttattagct
    20101 attttatcat atggttattt tggactttac cctcaatatc agaaatatat tgctacaaaa
    20161 aattctgtaa aacaagctac tgaaaaatta aatatatata aagagaaaac tgcacttatg
    20221 ccaaaatatg aaaaacaact tgaagaagtt gaaagtgaat taaaagaaaa aagcaaaaat
    20281 cttttatatg atatgcaaga tggtcttttt ttaataggtt tagataaaaa aatgaaggct
    20341 tataatattg acttaactag ttatgacgtt ggaaatgtta gagaatacga acatttttat
    20401 ggtgtatcaa caacaataag tgttagaggt gattatagaa atgtcaaaaa attaatggct
    20461 tatttagaag aacaaaaaaa tgttactcaa atactagatt atacaatgac ttcatatata
    20521 gaaccagaaa aagaagagga agaaacaaca aacaataagg atttaattaa aaatgtgaac
    20581 ataaataaag atacaaaaat atactgggtg gaaggcgaaa ataagtggca tttatataag
    20641 gattgtccat tgataaaaga gggagagatt ctaaaaaatg acaaactttt agataggtct
    20701 actagttatc tatgtaatac ttgtgtactt agagctgcaa cagaaaaaga agcaaaaaaa
    20761 gaagtcgtag ctccaaaggc agaaggtgta gtagatgcta catttaaatt tataatgtac
    20821 actaaagaga atactacaat gatacttgat acagataatc catcttcatg gagaccggga
    20881 aaatataatc catttaagtc tagtgcgaaa taaagctaag taagctttaa agataggtga
    20941 tgagtttgta caaaaataat gaaaaaggtc ttaccttatt agaagtaata atagctgttt
    21001 ttatactgac aatagtttta agcatttctt ataaagtgtt caatggtata acatcagcag
    21061 taaaaaggca acagattatt acagatgctc aagtaaatat taatctaatt aataagtatc
    21121 taaatagaga tttggaaaac tgtaaagagc taactaaaac tggttcaggt aataattatg
    21181 agtacaatat agagatgcca gataatgttg taaaatatga agtttctata gaaactaaaa
    21241 aacatacaga ggtatattct gtaacgagaa tccaaaaaaa tacaattgat acagaaaatg
    21301 aagttagaga agagataatc tataatcaac cactagttca aaacaataaa gaaatgaagg
    21361 aaacaccatt taaaatagaa aagcaaactg gtaaatctat ttatactgta agtatatact
    21421 ataatgaatc agtgcaagaa agtcataaaa atatcaattt aaataacaaa acttatacat
    21481 ttgatgttat gtcaagaata gggtaataag aatttattaa aaaataagta ggttgataaa
    21541 atatgagtaa aaagaatagt gaaagaggat tttccttaat tgaagtgtta gtagctatgg
    21601 ctattatggg aattgtacta tttgcatttt ttaatattat caatactaat aacaaagcaa
    21661 acactaaaaa tgacacagat ataacttcct taaactatgt ccaaagtgaa atagaaaatc
    21721 taagagaaaa gataaaaagt ggagaatttg attttgatag tttagataaa ctagaagatg
    21781 gaactgtcgt atatgaaaaa ttaatagata aatcaaaaaa agttgtatat gataaggttc
    21841 ttagtgagag tgacgtgagc ttatatgata ttccatatga aaagattaca acaataaaag
    21901 atgaagatgg taatttaatc gacaaagaaa atattacaaa taagataaag actattgtag
    21961 aagataaaag tggtcagata tataaagtag ctgtaactgg gaaaagcatg aatgactatt
    22021 caagtaaaaa agaggttaaa attgtaactg aaatatttaa agataaataa ttttacttgg
    22081 gggtgagatt ttgaggaagt ggaataaatt taaaagtgaa agaggggcag ctttagtttt
    22141 agttttggtt gtagtagcat tgctttctat tgttggattg atattttcaa atcagatagc
    22201 caatagaata aaatctacaa agactactaa tgagggtata caagcaaagt atttagcaga
    22261 gacatgtgtg gaaaatagta ttgataaagc atatgaaaaa ttgtatgatg agcttgagaa
    22321 aatggataat gagtttaaaa gtgaaaatca agaaaaatcc atatctaggt caaaattaag
    22381 aaatatatca gatgaagatt ttaataatca agatgaggaa aatattgaag cagaaagact
    22441 aggatatatg aataatataa atttctattt aaataaagct tcttcagaat tggaaaaagc
    22501 tagtatggaa ttgaaaaagt tatatgattt agatatgttg gactatagag acattgaata
    22561 tgtagatgca aatattattt cacatagaga ttctatctta gaaatttgca agaattatac
    22621 ttctggagat attagtaaaa taaatgaaca tattctgaaa gaagatatag actcaactac
    22681 attgattgaa gcaaagttag taaataatga tattctttta aaaatgtttt tagaagaaaa
    22741 taaaattgaa aatgaacatt taaattcagc cttttcacat acttataagg cgttagataa
    22801 catttctttg gcaatgcaaa atatgattga gtatagacat acatttcata tagatgagcc
    22861 aaaagtagaa gtatctaatg ggattccaaa ttctcaagaa tattatgaac taatacaaaa
    22921 tcctataata aatagtatgg agtatagctg gaatagtaaa tgggatacat tagaaaacct
    22981 attggaaatt ttaccaaatc aaactcaagg ttttaattct ttaagagttc atttgagaaa
    23041 taatgtaaga aaatttgaga agttaagcga taatatatct agtggtaaaa aaaatacagc
    23101 aaaaaatttc ttaaagtata aagagttatt gtatgaaata tcagaccaat gtaatcaact
    23161 aaaaagtatg tcttatgaga aaatacctgt taaatatgat aacatggctt tgataacaac
    23221 ttttgactat atacaaaatg agcttttagc agagataaaa tgtagattaa aagaattaaa
    23281 accccaagaa gttgataaaa cagaaggtat tagtataaaa ataccttttt ataaggctga
    23341 ctacgacatg acaaaagaag gatggcctaa attaaaagaa aatggttcag gggcggagtt
    23401 aagcttaatg gtaactggtg ataaagatgg aataaaagaa gtagaagtta cagatagtaa
    23461 aaaaaatatt attggtttag gtgttgaaga aaattcaaat tcaaagtata aggttgaggc
    23521 cattgtaaac tttaatctaa atatagatac aaatgtagtt ggaaattata atataaaaga
    23581 taaaattttg attaatcatg atataagttc atacaaaaaa gtaaactaaa tataaggagt
    23641 aacttagtat aaataaataa taaaataatt ttattattta ctactaaaaa aataagtaaa
    23701 ctaatagaag atatggagag taaatattat gtatatttat gatttattag agcaaggtat
    23761 aagactaaac gcatcagata tacacataac agttggaaca aatcctgtag ctagagtaaa
    23821 aggtggattt gtaaaactaa gtgaacaaat attgacatca gaagtaacaa tgcaaatggc
    23881 aaaagacata gcaggggaat ctatgtttaa agtaatagaa gaacatggag aagctgattt
    23941 ttcagcttct ctaaaaacag gagaaagatt tagggtaaat gcatataggc aaaaaggaaa
    24001 ttacgcaata gcaataagga ctataacagc agaaattcca acatttgaaa aattaggtct
    24061 tccagaaaat ataaaatctt ttactgaaaa gcataaagga ttagtcttag ttacaggtcc
    24121 aacaggaagt ggtaagagta caacacttgc aagtatgata aacataataa atgaaaaaca
    24181 acaaaaacat ataattacac ttgaagaccc gatagaatat gtacatcacc ataaacaaag
    24241 tttagtaaat caaagagaag taggaactga tacagaaagt tttcattcag cccttagagc
    24301 tattttgcgt caggacccag atgtaatact aataggagag atgagagacc ctgaaactgt
    24361 ttcaatagca ttaacagcag cagaaacagg tcatttagta ttttcaacac tacatacagt
    24421 aggggcagct aagacaattg atagaatagt agacatgttt caaccatccc aacaacaaca
    24481 aataaaaaca cagttatcta cagtttgtga aggtgtggta tcacagcagt tgcttcctac
    24541 agcagatgga aaaggcagaa tagcagcaat tgaattgatg tttgctactc cagcaataaa
    24601 aaacctgata agggaaggca aaacatatca aattccaaac atgatacaaa ctggagttaa
    24661 atcaggtatg aaaactatgg accaagattt aatggaactt tataaaaatg gaaagattac
    24721 aaaagatatg gcacttagta ggtgtactga ccaagagttt atgacaagaa tgataggtgg
    24781 agtaaactac aatggatatt ataatcgtta atctatatgt atttatagtt ggtatagtgt
    24841 ttggaagttt ctttaatgtc tgtattttta ggattccaga aggtatatct atttctagac
    24901 ctccctccca ttgtatgagt tgtaatacta gactaaagcc aatagactta ataccagtgc
    24961 taagctattt aatgagtggc agaaaatgta gatattgtta tgagaaaatt tctagtagat
    25021 atgctatagt ggaattaatt acaggaattt tatttttaac agtatttatg ttatatggaa
    25081 tatctacatc aactatttat tatttggttc ttgtgtcttt actgattata attacattta
    25141 tagatattga tcactttata ataccagata aaatactgat ttttggagct atattttcta
    25201 taatttttaa ccttttattt aaagaaattc cagttaagga ttcaattata ggtgttttta
    25261 tttgtggagg aagtgtctgg attatagttc ttttgattga atttgtaata aaaaaagagt
    25321 gtatgggtgg aggcgatatc aaactatttg caatgctagg tctttacatg ggtgttaaaa
    25381 atggtctttt gacagcttta ctaagcattt atgtgggagc agtctatgga atctgtgtaa
    25441 taattacaag cagaataaaa ggaaaggaat ataattctgt tataccatat ggacctttta
    25501 tatctattgg agcattaata acaatcttat gtggaaagca attacttgaa ttttattttt
    25561 ctatggttat atagatgata gtcaagttaa ataaagtttt aatttaaaaa aatgaattaa
    25621 agtatttaat aaggacaata ttattattaa ccctattact aaagtgctac aaactatgga
    25681 gtatataata ataatttcct tattttttgc acatttgata aataaatctc tatgtgtttt
    25741 ttgtgataga gaaaagaaaa atttatataa tacgtttttt atttacataa taactgtagt
    25801 tttatctgta ttaatatatc taaaatttgg gattagtgta gaatgtgtac aatatatggt
    25861 tttaattcca tttttaataa ttatatccat tgtagattac tacacaatat atgtatatga
    25921 tataacaata gttagtggta taatagttca aggtataatt tttttggtct ttttcaatat
    25981 agttcaattt gattatataa atcatatatt tggactattg ataggcttta ttatacctta
    26041 tattattgct aaactaacaa aaggattagg ctatggagat gtaggtctct atagcttatg
    26101 ttgctttgca ttgggaaata attatgggtt ttatgtgata gttttatcat ttattttagc
    26161 gtttttgttt tttattattt tatacataac taaaatattt aaagatgtag atataagaaa
    26221 gcaaattcct tttacacctt ttatttcctt gtcaacaatt ttaataatat ttacagagta
    26281 taatatattt aatttttatt atagtttgtt atacaaattt atataacagg aggtaagaaa
    26341 tgtacgtagt agtagggtta ggtaatccag gaaagaagta tgagaagact agacataacg
    26401 ttggatttga tgttatagat attcttgcta aagaatacaa tattagtgtt actaaaataa
    26461 agcataaagc tttaattggt gaagggagag taggtactga aaaagtatta ttagttaaac
    26521 cacaaactta tatgaattta agtggagaaa cattaataga tatatataaa tattataaaa
    26581 ttgatttaag taatatagta gtagtatatg atgatataga tttagaagtt ggaaagatac
    26641 gtataagaaa aaaaggaagt gggggaactc ataatggcat gaaatctata actaagtgcc
    26701 taggttcaaa tgactttcca agagtgagag taggtgtatc taaaccagaa gttggtcaag
    26761 acttagcaga ctttgtactt tctagattta gaaaagaaga atctgataac ataaatgaag
    26821 cattagaaaa agcggcatat gctattgact caataataag agaaaatata gatatgtcta
    26881 tgaacaaata caatggatag tgagagggag atttaaatga atgatgtttt tttatatcct
    26941 ttgcaaaatt caaaagagta taaagatata ataaactgta taaaaaatac aaaaggctca
    27001 ttattggtta atggactttt accagtacaa aaacctcata tatcgtattc tatatttaat
    27061 gatttatcac gacaaatgat ttttataacg agtagtgatt tagaagcaaa gaaagtatat
    27121 gaagatttaa gcttttatat ggaagataaa gttgaatatt taggttttca agatatttat
    27181 ttttatcatt tggatgcaaa ggacagaaac gaagaagcaa agaaattaaa ggtattgtta
    27241 aaactagcaa ataaagaaaa aataatttta gtaacttcta ttgaggcagt tttaagaaaa
    27301 tacataccaa aacaagtttt gctcgatagt gtatctcatt ataaggtggg agatagctta
    27361 gacttagaaa agttaactga gaaattagtt tcattgggat atgaaagagt ttcaaaaata
    27421 gaggggtttg gtcaatttag tataagaggt ggaattattg atgtattttc acttgaatat
    27481 acaaatccaa ttcgtatgga attatttgat gatgagatag attccatacg aacttttgac
    27541 gtatattcac aaaaatcaat tgataaattg caacaattct ctataactcc atctagggaa
    27601 tttatttatc ctgaaaaaac tacagatgca ttagttaaat taaaaaaaga aacaactaaa
    27661 aatactgatg aaggtacatt tcaaaatata gattatatat cgagtaagac ttattttgaa
    27721 ggtgtagaga actatataga ttatatttat ccagaagaaa acaaaagtat atttacttat
    27781 ttagcagatg atgcaatagt atttatcaat gatataacga gacttaaaga aagatgcgaa
    27841 aattatataa atgagtttag agaaaattat aaattaaatc ttgaaagagg tctggctatt
    27901 aaaaaccaag gtaaattgtt atatcactat acagatttag agtacttagt aaaagacaag
    27961 tctgttgtat taaactcgct attgccaaag tctataaata acttttctat aaaatctata
    28021 ataaattttg aaagtagaga agttcctacc tttaatggaa aggtagattt gcttgtagaa
    28081 gaattaaatc ggctgaaata taatggatac aaaataattt tagcaacaaa taccttagaa
    28141 agggcaaata aattaggaaa agatttattg gacaaaggct tagaaacgac catttctaaa
    28201 gatagagata ttgaaattaa atcttcacaa gttataattg tacctgcaca cataaacagt
    28261 ggttttcagt ataaatctat aaagtttgtt gttataacag acaatgaaat gataggtgtt
    28321 tataaaagag cctctaagac tagcaataaa aaagttaaaa agggaaagaa aatagaatcc
    28381 tttttagatt taagcgttgg agactatgta gttcatgaaa atagtggtgt aggtagatac
    28441 acaggaattg aacaaattac agtcaatgct attaaaaaag attatatgaa aatagtatat
    28501 caaggtggag ataatctata tgtgccaata gaccagatgg acaaagtaca aaaatatatt
    28561 ggtgcagaag tagaaaaagt taagttaaat aagcttggaa caaatgagtg gacgaaagca
    28621 aaagcaaaag ttaaaaaaga aattgaagat atgacaaaag acttgattga gctttatgca
    28681 aaacgtgaaa aaatacaagg gtataagttt tcaaaagata caccttggca ggcagagttt
    28741 gaatctttgt ttccatatca agaaacagaa gaccaattaa aggccataga agaaactaaa
    28801 aaagatatgg aatcaagtaa ggtaatggat aggctagtat gtggagatgt tggttatgga
    28861 aaaacagagg tagctattag aagtattttt aaagcttgta tggaccaaaa gcaagttgct
    28921 gtattggttc caacaacaat attagcccaa caacattata atacattcaa agagaggttt
    28981 gaaaattatc ctttgagagt agaagtttta agtagattta aaacaccaaa acaacaaaaa
    29041 cagattattg aagatgctaa aaaaggatta gtagatattt taattggaac acatagaata
    29101 atatccaaag atataaattt accaaactta ggtcttgtag tcattgatga agaacaacga
    29161 tttggagtaa aacataaaga agctttaaag aaaattaaat ctacagtaga tgtacttaca
    29221 ctttcagcaa ctcctatacc aagaacatta catatgtctt taagtggaat aagagacatg
    29281 agtgttatag aagaacctcc acaagaaaga catcctgtaa taacatatgt tacagagagt
    29341 aaggaaagcg taattcaaga tgagatagag agagagttaa gtagaggtgg acaggtattt
    29401 tttgtataca atagagttga acacattgaa gagatggcaa gtatgattca aaaactcgta
    29461 ccagatgcta gagtggctgt tgcacatgga agaatgactt ctaaatcatt agaaaatata
    29521 atcctaggat ttttaaataa agattatgat gtactagtct gtactacaat aattgaaact
    29581 ggtatggaca tatcaaatgc aaatacaatg attatatatg atgcagataa aatgggattg
    29641 gctcaacttt accaattgag aggtagggtt ggaagaagtt caagacaagg atatgcatat
    29701 cttttgtatg aaaaagataa aactttaagt gagatagcag agaagagatt aaaagctatc
    29761 agagaattta cagagtttgg ttctggtttt aaaatagcaa tgagagacct tgaaataaga
    29821 ggagcaggaa atattttagg ttctcaacaa catggtcata tggcagttat aggttatgat
    29881 ttgtacgtta aaatgcttaa tgatgcaatt aaaaaagtca aaggtgaacc tattgtggaa
    29941 gaaattgatg ttgaaataga tttaagtgta aatgcataca ttccagataa ttatatcaaa
    30001 gatgagttga ttaaaattga aatgtataag aaaatagctt ctattgaaaa taaagaagac
    30061 atgttggata tacaagaaga acttgaggat agattctctg atatacctaa accagtccag
    30121 acacttctta caatagctta tataaaatcg ttatgtaaga tattaaaaat agaaaagata
    30181 agacaattaa aagatgaaat attattagtt ccaataacaa agtatagaac taaacagaaa
    30241 ataggttata atatagttac agaattagaa gaattattag aaaagatgtg caaggtaaaa
    30301 taacttgtaa atttaagaag ggatgtttta aatttgaaaa aagtaataac attggtaata
    30361 actatgattt tggtagtatc tgtgactgct tgcagttcta gtaaaggaga aactgtggca
    30421 acagttgaag gtacaaagat atcatcagat gaatttaaaa aaactatagc tctttataaa
    30481 gattctatgg aacaaactta tggaaaagat atatgggata aggaagttga aaagggtgta
    30541 aaatacaagg acaagtttaa agatttaatt cttgaccaac ttataactac agaagtaata
    30601 tattctcaag ctaaaaaaga taacttactt cctaaaaaag aggatgttga aaaaagcttt
    30661 aaagaattaa aagatgctat gggtaaagat gaaaaatata aagaacagct taagaaatta
    30721 ggtatagatg atgagttttt aagagaccaa caagagaaag atttagcaat gcaaaactat
    30781 caaagtaact ttgctaaaaa gactaaaata tcagatgaag aaatgaaaaa atactatgat
    30841 acacataagg atgaatttaa aaaagatgag gtagaagctt ctcatatttt attgaaaaca
    30901 gtagatgata ataacaagcc actttcagat aaagaaaaag ctgaagctaa gaaaaaggct
    30961 gaagaggctc ttaaagaagt taaatcaggt gaggattttg caaaagtagc aaaaaaatat
    31021 tcacaagatg cttcagcaag tgatggcggt aaattaggat tcttttcaag gggtcaaatg
    31081 gttgctgaat ttgaagatgc tgctttttct atgaaaaagg gtgaaatatc tgatttagtt
    31141 gaaactcaat atggatacca cataattaaa gtgacagaca gaataaatga gcaaacatca
    31201 tttgaagatg ctaaagaaac tataaaagag caattgttga aaaataaata tcaagagcaa
    31261 atagaaaaat taactaaaga agctaaggta gaaaaagatg aaaaagtaat aaataaaatc
    31321 acaatctaag actcaaaaaa agagtaatat gcttaaaaaa gctatatctg gataagatat
    31381 agcttttttt gtatattttt cttaacaatg gtaaaaataa acttaaagta atttatggag
    31441 ggattaaaaa atatgaaagc aacaggtata gttagaagaa tagatgatct tggaagggta
    31501 gttatcccaa aagagataag aaagacatta agaattagag aaggagaccc tttagagata
    31561 ttcacagcaa aagatggaga agtcatactt aaaaaatact ctccaatagg tgaattaaat
    31621 gaattttcgc aagaatatgc tgaaacatta gctgaagtac taggatatgg agtgttgata
    31681 acagacttag attctataat agctgtatct aagcttccta aaaaagatta taaagaaaaa
    31741 agtataagtg atgagttaga agcaataata agtgaaagaa cagtaaaata ttcaaaagat
    31801 aaaaacattt taccattatt caaggaagac caaaaagaat atactagtca agttatcatg
    31861 cctataatta gtgcatctgg tgactgtata ggttcaattg tagttgtttc aaaggataaa
    31921 gatacaattg cagaaagtga agaaaaatct ttaaaaattg ctgctaactt tttaggaaaa
    31981 caagttcaat aattttatca taaagacctt ttacatgaag gtcttttgaa gtttttgtgt
    32041 aaaatattga atatagcatt aaaaatatgg tatattaaag tttgttatat aaacagtatg
    32101 gaggttatgt tatgaacaat aacaaaaata aagactcctt tctaaaagga gcccttatac
    32161 ttgggctagc aggtgtaatc gtaaaaataa tgggtggatt ttttaggatt ccccttggaa
    32221 acatgattgg ttcagaagga atgggatact atcaggctgc ttatcctgtg tatactctat
    32281 ttttaacatt agctactgct ggatttccaa cagctgtggc aaaattggtt tcggaaaaag
    32341 ttgccattgg aaattttaaa ggtgcaaata agatatttaa ggtatcacat acagttttat
    32401 tcattactgg tataatatca ttttgtatat tattttttgg agccgactac atagtaacaa
    32461 acgtaatgaa aaatccaggt gcattatact ctatgaaagc cattgctcca gcattactat
    32521 ttgttcctgc aatgtctgca tatagagggt attttcaagg gagacaagat atgactaaga
    32581 ttgctgtgtc tcaagttgca gagcaatttt ttagagttgt attaggtctt actttggctt
    32641 acttgcttat gaagagtcta gggcagactt ttggtgcagc aggtgctata tcaggggcta
    32701 caataggttc aatagcatct atgctttatt tggtgtttgc ttatatgcta gggagaaaag
    32761 agcgtagagc agagattgat gcaagccaaa gatttaaaga tgaaagagtg tcttatattt
    32821 ttaaaaagct tttaactgtt gccattccta taacaatagg agcatcagta atgccacttg
    32881 taaatatgat agataatgtt attgtaataa gaagacttat ggaagcaggc tttacatata
    32941 aagtggcaaa ttcaatgttt ggtcaattaa caggtatggc aatggccaca ataaatttac
    33001 cagcagtcat aacaactgca atgagtatga gcttagttcc agcaatttct aaagcatatg
    33061 cattaggaaa taagtcaaaa gctagaaaag atactaagag tgcagttaag gtgactttac
    33121 ttatagtatt gccttgtgcg tttggtatgg cttctttagc aataccaata atgggtcttt
    33181 tatttccaca tgaaccatct tctgtaggaa ctattttgtt tactcttact ccatgtgtat
    33241 tatttttagg attaatacag acattgactg gtataattca aggaatggga aaaccaatag
    33301 tacctgtaat tgctttatgt gttggtatgc tatgtaaaat tgtaataagt tatactttaa
    33361 caggtatacc agatattaat gtactgggtt cagcatttgg tacagttaca gcatactttg
    33421 tggcagctat gattaactta ctatacgtta aaaaacacat gaatgtaaac ttttctaaaa
    33481 aagaatttat aataaagcca tttataacag ttatgacaat gtttataatg gttaagttga
    33541 gttatggtgc tttagttgga ttcttaggaa acagcatatc tacaataatt gcaatatgtg
    33601 ttggtggaat tgtatatgta gtagtaatac ttggaatagg tggaattaaa aaagaggaga
    33661 ttttaactat gccaaaaggt gataaattat ataaattgtt aaaaaaagtt aaacttataa
    33721 gataatatag gaatatatat gcaaataaag gaaatactat acaaatagta aatctcctta
    33781 tttgtataat aatataaaaa taaagtgaaa tttgctaata ggagtgatgt tatgggtaag
    33841 ataagtattg taggtcttgg accaggagac tattctctta taagccaagg tgtgttagaa
    33901 tctttaataa atagccctaa agtttttttt agaacagaga aacatccaat aattgataaa
    33961 cttaaagaaa ctatacaata cacttcttta gactactttt atgaaaaaga ggaaaatttt
    34021 gataatgttt atttgaaaat atctgaattt atcatagaag aatccgcaaa gggtgaccta
    34081 gtgtatgctg ttcctggtca tccaagagtt gcagagaaga ctgtaagtat aatagagaga
    34141 ttagctaatg aaaagcacat agaagtagag atattgccct ctatgagctt tgtagacgct
    34201 atgtttaact atttggctat agacccatct gaaggattta aacttgtaga tgcttttgaa
    34261 atagaaaatt catatataga cttggataca agcatgataa taactcaggt atatgataaa
    34321 tttatagcat ctaatgtaaa gttgaaactt atggaacatt atgactataa tcaagaagta
    34381 tgtatagtaa atggagcagg tgtcaaagat ttagaaagta aaaagtttat aaagctttgt
    34441 gaattagaca gaaatgaaaa cttttttgac tatttaacta gcttatatat acccaaaagt
    34501 tctaaaaaga tgtataatac agtacatgac ttagaaataa tagttaatac acttagaagt
    34561 ccatctggat gtgaatggga taaaaaacaa acacatcaat cattaaaaaa ttctgttata
    34621 gaggaagcat atgaactttg taatgcaatt gataataatg atatagatga aatggtagaa
    34681 gagttaggtg atgttctttt acaggttata ttccattgtc agataggaaa tgaggagggc
    34741 tactttgatt taaaagaagt agtaaatggc atatgtaaaa aattaattca tagacatcct
    34801 catatattta gtggtgcaaa actagatatg tctaagtttg aaaaaacttg ggaagaatta
    34861 aagaaagaag agaaaggcga aagtactata acagatggcc ttagaaggat accaaaacat
    34921 ttaccagctc ttatgaaggc tagtaaagtt caacataaag cagcacttgt cggctttgat
    34981 tgggacaatg tagaagatgt atttaaaaaa attgaagaag aatatcagga attacttgac
    35041 gaacacaggg tcgggaatat aaaatacata aaggaagaac taggcgattt attattttcg
    35101 atagtaaatc tagctagatt tttagacata gactcaggag aagccttgaa ctgtactaca
    35161 gaaaaattca taaatagatt tgactttata gaaaaaagtg caatgaactt gaataaaaaa
    35221 atagaagaca tgacacttga agagatggat gagttttgga atcaggctaa aaaacaataa
    35281 aattgtggaa ggaaatttgt caaaaatcta gaacttttat cacaaagata gaagtctata
    35341 ttataaacat ttttaggagg taaatatcgt gaataaagct gaattagtat caaagatggc
    35401 agaaaaaagt ggattaacaa agaaggaagc agaagctgca ttaaacgcat ttatgagttc
    35461 tgttcaagat gcactagtaa ataatgaaaa agttcaatta gttggatttg gaacatttga
    35521 gacaagagaa agagctgcta gacaaggaag aaatccaaga gatccagagc aagttataga
    35581 tataccagct tctaaagcac cagttttcaa agctggaaaa ggattaaagg atataataaa
    35641 tggataatta gaaatcttgt gaaaaagtgt ggataaattt gccacacttt ttctatagag
    35701 ccaaggaagg tgataattat gagattagat aaatttttaa aagtatctag gattataaaa
    35761 agaagaactg ttgcaaaaga tgcatgtgat aaaggaattg tcactataaa tggaaaagtt
    35821 gctaaatctt cttcagaagt gaatattgga gacacattag aaatacaatt tggagagaag
    35881 aagatgaagt ttaagataaa tgaagtaaaa gagcatgtat taaaaaatga tgcaaaagaa
    35941 atgtacgaaa ttttagaata aaattaatag tcctctagta tattaatagt agtattattg
    36001 aaatatttac tggggggata ttatggaaca gaacataact ttgaaagata gatcaaagct
    36061 ggttatttcg ggagttgagc acatatactc ttttaatgac aaaagagtag agcttaaaac
    36121 ttctgtaggt gagatggtaa ttgaaggcga aaacctggat atgagcaagc taagtataga
    36181 cgaaaatata attagcatag atggaactat aaattctatg gtatatgcaa agcctaaaaa
    36241 gccacaagag agtttcttga aaaaggtatt tagatagatg atacctttta ctcaagatgt
    36301 aagtattttt tatgctacta tttatggtgg aatactcata ggggtgttgt ttgattttta
    36361 tagagggcta agaggaaatt ttaaattcat caattatttt gctataatct ttgatgtatt
    36421 gttttggttt ctagctacgg ttataatatt cgtaacaata aaccttacag aattttttga
    36481 tttaagatat tatcattttg tggctttgtt tattggtttt attttatact ataacaccat
    36541 aagtaaaatt gttttatcaa ttatcaataa aataattcgt tttgtgagaa attcttttaa
    36601 aaaagttaca cattacatag tcagtttttt aaataatttg tattacgtta taatctattc
    36661 actacaccta ctatttgata ttatatttta tataccaaac atatttatag caacaagaaa
    36721 aagtataaaa agaaaatcaa ataaaaaatt aaagaacaag aaaaaatcaa aacctaagaa
    36781 gaaaaaaaat aaaactaaaa aaagggtgta ggaaaaatga acttaagaaa aaaattttca
    36841 ggacagatta ttgttatcag tttattttta ggaatatcaa tttttagtat gatgacagga
    36901 tttgtatttg agtatacaaa agctaaggaa tataaaaaag aaatagcaag tctaaataaa
    36961 caattaaaaa agactgaaat acaaataaac aacttaaaga aagatgaaaa atcatatgaa
    37021 ggagacttag aggatatagc aagaaaaaga ttgaatatgg taaaaccaaa tgaaacagtt
    37081 tatgtagaca taaatcgttg attaaaactt gctaataagt ttacagatgt attgatttta
    37141 aaatttaaga tttaataaaa aagtcattca gttaaggatg gctttttgtt tttattgagg
    37201 agggaatttc ataatgaaag taggagctat agatattggg acaaactcaa tgagattact
    37261 gatagcagat tatagagatg ggaattttat aaacagaaaa aaacttgtta atacaactag
    37321 aattggttat ggtgtggata gtaaaggata tataaaagaa gatgctatca aaagaaatat
    37381 aaaagcatta aaagaatttt ctgataagtg tagtgatgaa aagtgtgaaa aaatttattg
    37441 tatggggaca tctgctctta gagacagtaa aaatggagat tactttgtta aattagctaa
    37501 ggaagaaact ggaatagatg ttgatatagt tacaggaata gaagaatcaa atttgggctt
    37561 taagggtgtt ttagaaggaa tacaaggtga tgatactatt attgttatag atattggagg
    37621 aggttcaaca gaatttattg taggtgattc agaaggaatt aaatttaata aaagtgagaa
    37681 tgtaggtgct cttaggatga cagagagatt tttgaagcac gatattattg aagatgaaga
    37741 gtttgaagct atgagttcat ttatatatga tgagattgac aaaacgttaa atcatataaa
    37801 agacagagga ttaaaaactc ttataggtat aggaggtacg attacatcat tatcagcaat
    37861 gaatcaagaa ttagaggttt attctatgga aaaaatacat aatagtaaaa tacacaaaaa
    37921 agaaatagaa gaaattctac aaaaattaat aaagatgaca ttaagtgata aaaagaactt
    37981 aaaagggcta caagaaaaac gtgcggatat tataactgct ggtgtaaaaa tattgaatat
    38041 aataatggaa aaattagaaa tagaagaaat aaccgtaagt gaatacgata atttggaagg
    38101 cttaatatgt caaaagtcaa aaaaaatgta ttaatatttt ttgattttaa acaccgatta
    38161 tgacaaaaaa aattactcct ctttgttaca attcttaata taaacaaaag aggaggtttt
    38221 tttatgcaaa gaagtgtagc agttgtgaag aataagactg taaaaccaaa tgtcataaaa
    38281 ttaataagcg gatatattga ttttactacg gcggcattcg ctgtgatggg atttttactt
    38341 agtaggtcta ttatagtaga ctctgtagct cctttaggaa ttgccttttt tatatgtgca
    38401 tcaaagattg ataagtacaa gatacctgta ttcatatcaa cactattagg aatattgtta
    38461 tcatctaatg attttggaaa tgtagcaaaa tatgctgtgt gccttacaat atttatggct
    38521 atgaataaaa aattaaaatt atcagattca attgctaagg ttgcatttat aggtgcaata
    38581 atactacttc caatctccat gggacaagct ttcttttcaa ataggtatgt gtatggtctt
    38641 cttatagcag gagtagaagc aataataatg tttatatcta tatatgtgtt ttcttatgga
    38701 ataggtttaa tggccaatgt caacaatagg atgtctgtac ggacagaaga agctatatca
    38761 ataagtttac tgctaacctt tagcataatg ggaataggag atatatccct gtttggaata
    38821 tcaataagga ccattttatc gaccgtattg atattgctga cctcaatagt aggtggagct
    38881 accatgggag catctagtgg tgtaattatt ggtgtagcat ccattttaaa taatatgaca
    38941 tcagctattt atatggggat atattctttt tcaggattaa taagtggagc atttaataaa
    39001 ataaataaat atttctgtat actaggatat attctaagtt ggacaataat atacctatat
    39061 acttctggta ttacttctaa tatgatgcaa ttaagagata tactattagg ttgtttgata
    39121 gtactagtat tgccagagag attatttaat aagattgaaa aattaataaa aagtaatgta
    39181 gcttcaaatg aaatcgtata tgattatatt atgagaagca aaaacctaac taatagcaga
    39241 ttgaacagta tttataaaac ttatgatgac cttgcagata catttgataa gataagagaa
    39301 aaagacaaag tgctagacca aagagatata gctaatgtaa tagatatgat tcataatgat
    39361 gaatgtaaaa gttgtagtat gagaagaatg tgctgggagt caagatttaa tcatacatac
    39421 actatgatat acaacattct tgaaaaaatt gaggagaaag gggagttaag cctaaacgat
    39481 atacctaaaa attttagaaa ggaatgtatg aaaccagagt caatagtgaa aatatcaaat
    39541 cactattaca aaatgtttgt attagattat gattggagtg tgaagttttc agaaagtaga
    39601 aagttaatag caaatcagat aagaagtatt tcaaaatcca ttaaatcgtt atctcaagat
    39661 ttagaaggcg acataatgct agatatagaa aaggaaaaaa atatatatga acagttagaa
    39721 agatatgata taacagttga taaagtaagt tacttaacaa aaagtaacag tgagtttgaa
    39781 atatcaatag aaaagaaaac ttgccatgat ggatgtatgt gtgaagataa aattgtaaat
    39841 ataatttcag acctagtagg tgaaaatatg tctgttagaa aaatagggtg ccattgttta
    39901 ggaggtaaat gtaaggcaac attcgttaaa tcccaaaaat ataaggctgt tacagaagtt
    39961 tcagcaatgt caagggatgg gcatattctt tgtggagaca attacacata catggagata
    40021 aatgatggta aatacatgat ggctataagt gatggtatgg gaaaaggtaa aaaagcatat
    40081 gaagaatctt ctgccactat tgatatactg gaaaaaatga tagatgcaaa aattaaagat
    40141 gagatagtta ttgatactat aaataatatg ttgttgttaa agtcttcaga agagatgttt
    40201 tcaactctgg atttgggaat tttagattta aaacgaggtt gtttagaaac tataaagatg
    40261 ggagcgtgtt ctacttatat aaaaagagag gatggagaag ttgacttaat atcgtcatca
    40321 tcattacctg tgggtatatt atcagatgta aaaattgata gaaaaaatgt aaaagttaag
    40381 gaaggagact atgttataat ggtttctgat ggaattgtag atgcaggaag aaataacaat
    40441 ttaggagaca attggttaat atacttctta aagaatatag agactacaaa tccaaaggaa
    40501 atttcaaatt taatattgga tagagcatta gagttacaag cgttacaaat cgaagatgac
    40561 atgactgttt tagttacgaa aatatgtaca aaataagggt tacaaaatta tgtgtataag
    40621 atgtggataa cttgtgtata atatgttaat agacttttga aaaaagtata gttattaaca
    40681 aaaataacat ataaatcaac aaaatttgtt gataagtatg gcagaaatgt taataaaatg
    40741 taaaaaaaga aaaaacaaaa gctattatca aaagagattc atgttgaaat aactaaattt
    40801 ctaagtaaag aaaacctgtg aatatgttga ttattatgtg gataactatt aattcgatat
    40861 ttatttatat agtatgaata attgaataat atatctaaaa aaagccatcc tattattaag
    40921 taaacttatt gaaaggatgg ctttttatga atagaaaatt gtgcaaagca ataatacctt
    40981 tatttatttc tattttttgc atgttaataa taagtataaa agctgaccaa ggatatcaaa
    41041 agaataatga tagagcaatt actgtcatga ataataatga tactgaagat gatgctgaaa
    41101 aaaaacaaga aatagactat gaaaaaacat gttggaattt gcaatctttg tttaaaagtg
    41161 atgaccaatg gaaaaaagaa cttaagagtt ttgataaaga tactaaagag ttgaaaaatt
    41221 acataggtaa agtaacaaaa tcgccaacac atttgtcttt tgccttaaac attaaagaaa
    41281 aacttgatat aagactaaat aaactttctg cctatgtaaa attaaaacaa gatactaata
    41341 aaaattcata taagtactta gatatgaatg atagtatgag taaatcctat gggaattata
    41401 tgggaatttg ttcagattta gagttagaaa tactaaaatt atcaaacaaa gattacaaga
    41461 aaattatttc aaataaaaat ataaatagga aatatggtgc atatttaaga gatataagaa
    41521 ggaataaagt acattatttg gatgataaat cagaagatat tctaagtaaa gtatcttcaa
    41581 taagtagtct tccaagccaa gtatatgaat tatttagaaa tatggataga aaaactaatt
    41641 tgacaccagc tcaatatgca agtgaattag agtcatcaga tagagaaaat agaaaaaaag
    41701 cttatcaaga tgaacttata acttacaatg ataatataaa tacaattgca ggtttaatta
    41761 ctggtcaagt taaaaagaat attttttatt ctcaggaaag aggctatgag tcttctttag
    41821 aaatgtactt ggaatctgat aatgtagatg aaaaagtata caataatttg ataaggactg
    41881 taaatagtaa tatggacagt ctacacaaat atatagattt aagaaaaaaa gtattaaaat
    41941 tagataaggt tcattcatat gatatgtttg tacctatggt taaggctgtt gataataata
    42001 ttaattatga aaaggcacaa agtatagtat actcagcgtt gtcaccttta ggaaaagaat
    42061 acaatgatgt agtatataaa gctttcaatg aaaaatggat agatgtttat tcacatgaaa
    42121 ataaagtaag tggtggatac tgtttatctg tttatgaaaa tcatccatat atacttttaa
    42181 attataataa ttcattaggt tcagtttcga ctctatcaca tgagcttggt catgctgtat
    42241 acgaatactt aagttctaag aatcagaatt attttaattc aaatccgtct atatttactc
    42301 acgaagttgc atctacaacg aatgaagcat tactttatga gatgcttata aaagaagcca
    42361 aaggaaatga tgaaaaagct tattatataa cacagtattt agacttaata aaagatactc
    42421 tttatacaca aactatgtat gctgaatttg aaaaaactat acatgaattg gtagaaaaag
    42481 gaaaaagcgt aaacgcatta gtattaaatg atgcttgggg acaacttctt aaaaaatatt
    42541 atggacaaag ctatgaatta gaccaattat ctaaggtcgg atgggctaga atcccgcatt
    42601 tttataatag tttctatgtc tataaatatg ctacaggttg tagtgcagga gtaagttttg
    42661 cccaagacat cctaaagaat ggttcagata actatatgag ctttcttaaa aagggaagtt
    42721 cagattatcc aataaacttg ttaaaatcaa gtggtataaa tttaactaat acaaaaccca
    42781 tagaaaatac aatcaaaaaa tttgatagtt tagtagcaga acttgaaaag ctattataaa
    42841 cattattgta atccacatgg taaatatatc gaattaatga taaaatatac taaaagttta
    42901 aagtcctatc aagataggac tttttattta ttaattagtg ggagatgatt ctatgcaagt
    42961 taaagttaaa aaggctgtaa taccagcagc aggtttagga actaggtttt taccagctac
    43021 aaaggcacaa ccaaaagaga tgttaccaat agttgataag ccaactctac aatacataat
    43081 agaagaagct gtggcctcag gaatagagga aatactaata ataacaggta gaaataaaaa
    43141 atctattgaa gaccactttg ataaatctgt agaattggaa cttgatttag agaaaaaagg
    43201 caagaaagaa ttgttagagg tagtacaaaa catatctaac atgattaaca tacattacat
    43261 aagacaaaaa gagccaaaag ggttgggaga tgcaatttat tgtgcgaggc attttatagg
    43321 tgatgagccg tttgcagtaa tgttaggaga tgatattgta gataatgatg tgccttgtct
    43381 aaaacaacta acagatgcat atgaggaata tagaactaca atattgggtg ttcaaaaggt
    43441 aaatcaagaa gatactaata aatatggtat aatagaagca aaaaatatag agggtagagt
    43501 ttataaggta aaagatatgg tagaaaaacc agagtcagga aaagctcctt caaatattgc
    43561 tattttgggt agatatataa taactcctga aatctttgat attcttaagg atttaccacc
    43621 aggtaaaggt ggagaggtcc aacttacaga tgctttaaaa atattatcta aaaaagaagc
    43681 tatgtatgca tacaattttg aaggtaagag atatgatgtt ggggacaagc taggattttt
    43741 agaagctact gttgactttg ctctaaagaa agaagacctt aaggaagatt ttataaaata
    43801 tttgaaacat gtatgtagtg aatttgataa aaataacaat gtgttataca ataatacaga
    43861 tgaaaaaata gatttaattg aagccaatgg agctagagaa cttgaagttc aaaaataatt
    43921 aatgaaaggt gatgtatggt ggcaaaatca agtcagaaaa gaattaatag aataatagct
    43981 gttgggattg tgggaatcct cgtattaacg agtataacag ctgttttgag tatgattgca
    44041 gctatgtaat aagataaatg acagcagttg atatacataa atataaaaac catatataac
    44101 ttgatgtagt taaaaactta caatatttta gttatatatg gtttttagaa tttataaatg
    44161 taaaaaaaaa tttttataat agttgcatat tttaaaggaa aatattatta taagatataa
    44221 atatagtgat aaatttataa aaacgtgcta agattggact tgaaatacag taatatagta
    44281 ggtaataatg ttttaaattt tatttattga ccataatata taaataatga tattattaga
    44341 cttaactgtt taaataaaac ttttcttttt catgccttca atttccaaaa taagtatgta
    44401 tataaatgaa tatatagatg aggaggattt ttttgatttt agatgataaa gtattagaag
    44461 gattaggtag taagacctta gttgagctac gagagatagc taaagaatta aaaataaaat
    44521 caattactac atataagaaa aatgagttaa ttgagattat aaattccaaa agtaaaaatg
    44581 aggaaaaagt aaacgaagag actaaagaaa aaaacgaaga tattaaagaa gacataagga
    44641 atagaaaaga aaatatagag cgtgaaagta tagaaaaaga agttgaatat aagagcccta
    44701 ccaaatcata caacaagaaa gaaatagata cccaaaatat ttcccaaaac caagaaaata
    44761 gaaatcaaag aaatgactat aataagacaa cgagtgtaaa tgatagtaat aagtcatcaa
    44821 atatatctaa tagaatggtc agaaataata ataaaaacta ttatatgcct aaacaagttg
    44881 atgaatctaa gatagtagat gaatttaaca catcaaaaga ggatgaagta gtaggtgtac
    44941 ttgaaattct gccagatgga tttggttttt taagaggttc aaattatttg tctactgaag
    45001 gagatgttta cgtttcacca tctcaaataa gaagatttaa tatgaaaaca ggagacaaga
    45061 ttaagggaat aactagacat ccaaaaagtg gagagaaatt tagagcactt ttatatgttc
    45121 aaaaaataaa tgatgaaaac cctgacacag ctatacaaag aaatgcgttt gagacattaa
    45181 caccaatatt tccagaagaa cgacttacac tggaaactaa tagaaatgaa atagctacaa
    45241 gaattataga tttaatttca cctataggaa aaggacaaag aggattgata gtagcacctc
    45301 caaaagctgg gaaaacagtt cttttaaaaa gcgttgcaaa tagcatagct aaaaatcatc
    45361 caaatgttga acttatagta ctccttatag atgaaagacc agaggaagtt actgatatga
    45421 aagaatctat tgaaggagac gttatatatt ccacttttga ccaagtatca agtcatcatg
    45481 tcaaggttgc agagatggtg ttaaatagag ctcaaagatt agttgaacat ggtaaagatg
    45541 tagtaatttt actagatagt ataactagat tggcaagggc atataacctt acaatatctc
    45601 ctacaggtag aactctatct ggaggtattg accctggtgc acttcatgga cctaaaaagt
    45661 tctttggtgc agcaaggaat attagacaag gtggttcttt gacaatatta ggaacagcat
    45721 tggttgaaac cggttctagg atggatgatg taatatttga ggaatttaaa ggtacaggaa
    45781 atatggaact acatttagat agaaaattag cagaaaaaag aatattccct gcgatagata
    45841 tttacaagtc tggtacaaga agagatgatt tattgcttga tgatgaagaa aagactgctc
    45901 tttggagatt aagaagagaa atgagtaata attcagtaat ggaaattaca gataaagtca
    45961 tcgaacttat aaagagaact aaggacaata aagagtttgt aaaatcaata aagaatttgt
    46021 aaaatcaata atacaaatgc aaatgtatgt tatataaact aatagaaaaa tcacttgaat
    46081 ggcataataa gttgtgctat aataatgtag ttgataatgt aaaaagaaag ttaatataat
    46141 taattttttt aaataaagag aagaggtgac tataatgcaa aaggaaatac aaccaaaata
    46201 caatccagtt gaagtgcgtt gtgcttgtgg gaacacattt gttgctggtt caactaagga
    46261 cgaaataaaa gttgaaatat gttcagagtg ccatccattc tatacaggaa aacaaaagaa
    46321 catagaaaaa ggtggaagaa tcgacaaatt caagaaaaga ttcaaaatgg actaattttt
    46381 ataaaagtgg agttggagga agccaactcc tattttatta caaaaaaaca ttcatattct
    46441 actttaaatt gtctttttgt attataataa taaatatgta aaattgagta ttctaaatac
    46501 aataaaatga ggtgagtgga tggcaaaaca agctgtaggt gggcaagctg ttattgaagg
    46561 tgtaatgatg cagtcaaaag acaaaagagc ggtagctgtt agaaaaagcg acggagaaat
    46621 agcactaaaa gaagatagaa taaaaagttg ggtaagagat aagaatatag ataagatacc
    46681 ttttattaga ggttcttttg ttatgataga tacaatgatt caaggcataa aaagcttaaa
    46741 cttttcttca gagtttttta tggaagaagc tgaagaggat aaatttgatt tatttataaa
    46801 gaaaattttt aaagataagg caaatgatat aattataata ttttcattgg tcatagcaat
    46861 gttactttct gctggattat tcatttttat acctacattg gtaggaggag ctttttctaa
    46921 agtaatgcct aatgatttta tgcttaattt aatagaagga atcataagga tagctatatt
    46981 atttgcatat atagtattga tttcacgaag taaggatata gaaagagtct ttcaatatca
    47041 tggagcagaa cataaatcta tatattgcta tgaaaatgat ttagaactta cagtagagaa
    47101 tgctagaaaa tttaaaaggt tacatcctag atgtggtaca aatttcctat ttatagttat
    47161 ggctgtatca ataatattgt ttgcattttt tggttggcca aatccaatcc ttagaatatt
    47221 tatgagaatt atatgtgtgc ctatagtagc aggtttatct tatgaagtaa ttagagttct
    47281 tgggaaatat gataatggat ttactaaaat tattgcatat ccaggaatga tgttgcaata
    47341 ttttacaaca aaagagcctg atgatgagca attagaggtt gctttggaag cattgaaggc
    47401 agtagtagat taaagaaaag gatatttgtt atgacaataa aagatataat tattaaatat
    47461 tcagataaac taaaagatat aagtgatact ccaagattag atacagagtt attgttacaa
    47521 aaaacattgg gtgttgatag gttatatatt cacttaaatc tgaataaaga gctaactgaa
    47581 gaacaaaaaa caaaatttat aggttttgca gaagaaagat taaatggaag accaatagca
    47641 tatatagttg aaaataggga gtttatggga ttagattttt ttgttaagga aggcgtattg
    47701 attccaaggc cagatacaga aactttagta gaagagataa ttgaaatatg tagagaaaaa
    47761 aaagatgtaa gtatattaga tataggaact ggttctggag ctattacaat aagtttagca
    47821 aagtacattg aaaattctaa aatcatgtct tttgatgtat cagagatagc attagaaata
    47881 gctaaaaaaa attctattat aaatgaagtt ggtgaaaaaa taaagtatat taattctgat
    47941 ttatttacag caataagtga tagtaacata aaattcgata tcatagtttc aaatccacca
    48001 tatattaaaa aacaagatat agaaactctg cacaaacaag ttaaagatta tgagccatat
    48061 aatgctcttg aaggtggaga agatggatta gacttttata gaagaataac agaacaaggt
    48121 aagaaatatt taaataagcg tggaatatta gcatatgaag ttggtcataa tcaagctgaa
    48181 gatgtaataa atattatgaa aagtaatgga tataaaaaaa tatacacaaa gaaggatatt
    48241 caaggcattg atagagttgt tataggttac aatatatgat ataatcaaca attgatatgt
    48301 tacatttaat cgaggtgaaa gtatgttaaa aaaattagag gtattggaag atacatataa
    48361 agacttaagt gaaaaaatag gtgatccgga tgtaataaat gatcaaaaag tttggcagaa
    48421 atatataaaa gaacatgctg acttagaacc tattgttatg aaatacagag agtataaaag
    48481 cgttttagat agtataaaag aatcaaaaga aattttacaa gaagagtcag atgaagaatt
    48541 aagagaatta gctaaaatgg agttagctga gatggaagaa aaagtagcac ctctggagga
    48601 agaaataaaa atactattat tgcctaaaga ccctaatgac gataagaacg ttatagttga
    48661 aattagaggt ggagctggag gagatgaagc agctctattt gcgggtgatt tatttagaat
    48721 gtactcaaga tatgctgaaa gaagaagatg gaaaatagaa ctattgagtg caagtgatac
    48781 tggagttggt ggatataaag aagtatcttt tatgataaaa ggtaaaggtg cttattcaag
    48841 attaaagtat gaatctggag ttcatagagt tcaaagaata ccatcaactg aatctggtgg
    48901 aagaatacac acatcaactt ctactgttgc agtattacca gaagttgaag atgtagaggt
    48961 tgaaataaat ccaaatgatt taagaataga cgttttccgt tcttcaggta atggaggaca
    49021 aagtgtcaat actactgact ctgctgttag ggtaacacat atacctacag gtgaagtagt
    49081 atcttgtcaa gatggaaaat cacaattaaa aaataaagag caagctttaa aaatattaaa
    49141 agctagactt tacgataaag cattggcaga acaacataaa gatatagcag cagaaagaaa
    49201 aagccaagtc ggaacaggag acaggtcaga aagaataaga acatataatt tccctcaagg
    49261 tagaataagt gaccatagaa taaatttaac tttatataag ctagatgcat tcttagatgg
    49321 agatatagat gaaatgatag atgctttaat tactgttgac caaacagaaa aaatgactgc
    49381 aatataagta atgttttaaa gaagctgttt ctttctagaa attattttct attttgaaac
    49441 agctattttg cattttaggt catgttatca tattctactg tatataattg ttatatacaa
    49501 gtataggagg gttatatgat actaaaggta acaataattg gacttttggc aggtgttata
    49561 gggacaggat taggaggagt aatatcagct atttttaaaa gagaggtcga taaatatcta
    49621 agttttttta tgggattatc tggtggaata atgttggctg tagtggtgtt tgatttgatg
    49681 aaagaatcca tggataaaat gggaataatc aatacagtta tatttacatt tgttggagct
    49741 ctcataacta tgtacataaa aacaaaatta gatgtgtctg gaaatatggc atctggatat
    49801 cttatattca taagtatatt actacataat ttaccagaag gactagccat tggttcttct
    49861 ttcatgtcaa ctgaaagttt aggtattaca cttgctatag ttataggtct acataatata
    49921 ccagaagggt tagctatggc attaggctta gtttgcaata aaatgaaact aagtaaagtt
    49981 attttgttta ctgtaatagc tggattacct atgggtctag gaagtttttt aggtgtttat
    50041 tttggaggag tatttacttc tcttatagga gtatttcttg ctacagcagg aggtactatg
    50101 atgtatgttg tattggaaga aatttttcca cattcaaaaa gtgtgtattg tataattggt
    50161 ttcttgttag gtacaatgat tgtaaattat atataaaatt aaaatgaaag caggcataca
    50221 aaagatgaaa actattataa gtaatattga tataaataat attgattatg aggaaataaa
    50281 aatacaagca aaacttctta gagaaggtaa aacagttatt tttccaactg agacagtata
    50341 tgggttgggt gcaaatgcat tagatgaaaa tgcagtaaag aagatttatg aagctaaagg
    50401 aagacctagt gacaatccat tgatagtcca tatatatgaa aaaaaagaag tatatgactt
    50461 agccaaaaat ataagtgata aagctaaact tgttatagaa aagctttggc caggacctat
    50521 aacaattata ttaaataaaa aagatataat tccatacaaa actagtggag ggcttgatac
    50581 agttgcgata aggatgcctt ctaatattat tgccagggca ataataaaag aagctggtat
    50641 acctattgca gctccttctg caaatatctc aggtcgccca tctccaacaa aagcaaaaca
    50701 tgtatatgaa gagatgaatg gaagagtaga tggaatagtt ttaggaggag acagtaattt
    50761 tggattggag tcaactgtat tggatttaac agaagaaact cctatgatac tgagaccagg
    50821 aagtataact aaagaagttt tagaggaact tcttggagaa gtaaagcttg acccatcatt
    50881 aagtaaaaaa gaagataatc aaaaagctaa agctcctgga atgaaatata aacattattc
    50941 tccaaatgca gatgtatata taatatctgg aaaaagagaa aatgtagcaa ccaagattaa
    51001 tgatatgata aaatctaata gagacaaagg tctaaagaca ggtgtaatgt gtattagtag
    51061 aaaccgagag ttttatgatg gagaagtaat cgatttagga aactctcttg aagaagttgc
    51121 aagtaatctg tttgatgctc ttatagaaat ggacaaaaag ggtgttgatg taatatattc
    51181 agaagagttc cctaagactg gagttggaca agcaattatg aatagactac tgaagtcagc
    51241 aggatacaaa gtaataaagg cttaagaggg agttgtaaaa tatgaatata cttattgtgt
    51301 gtacaggtaa tacttgtaga agccctatgg ctgaggctat actaaggaaa gcaattaaag
    51361 aatctggaag aagtattgaa gaatattcta tatcatcagc aggtatatct acagcaaatg
    51421 gaatgagtgc ttctgaaaat tctatagaag cactaaagga aatcggtata gacttatcaa
    51481 atcataaaag taaagtaatt actaaaaaat taattgatga atcagatata atactaacaa
    51541 tgactaaatc tcataaagaa attttagtac aagcagttcc aaaatgcaaa gaaaaagtgt
    51601 atacttttaa agggtttgca aataaaaatg aggaagatat atctgaccca tttggtggaa
    51661 atttggatat ttatagaaac actatgagag aaataatgta ttctgtcaac gaaatagtaa
    51721 aaaaaatatg aaacggagga taaacaaatg aagataggat taggctgtga ccatggagga
    51781 tataatttaa aaaaagaaat aataagctat ttagaaggta aaggtataga atgtgtagat
    51841 tatggaacta ataatgcgac agattctgtt gattatcctg tttatggaga aatagttgca
    51901 aattcagtta taaataaaga agtagattat ggaatattat gttgtggtac aggaatagga
    51961 atatctttag ctgctaataa ggtccctggg ataagatgtg cagttgtatc agatgtattt
    52021 tctgcaaaaa tgtcaaaagc acacaatgat gcaaatatgt tatcattagg cgaaagagtt
    52081 ttaggaaaag gtctagcact tgagatagtt gaagcatgga ttaatacaga ttttgaagga
    52141 gatagacatg ctagaagagt aaacatgatt aaatcaatag aagaaaaaca taataaataa
    52201 taaaaattac agttttatca ggaggaatta tcaaaatgag caaagtggta gagacaaacc
    52261 atccattaat acagcataaa ttaactttaa tgagagataa aaatactggt tctaaagact
    52321 tcagagagct tttaactgaa attgcaatgt taatggggta tgaaataaca aaagatatac
    52381 cattaaaaga tgtagaaata gaaactccta ttcaaaaaac atcatctaaa gtagttgcag
    52441 gtaaaaaatt agcaataatt ccaattttaa gagcaggtct tggaatggta gatgggttag
    52501 taagcttaat gccagcagcg aaagttggac atgtaggact atatagagac ccagaaacat
    52561 taaaaccagt agaatattat tgtaagcttc ctcaagatat aggagaaaga gatataatag
    52621 tagttgaccc tatgcttgca acaggaggtt ctgctgtagc agctatagat ttacttaagt
    52681 ctaaaggagc taaaagtata aaattggcta atttagtagc tgcaccagaa ggtatagaag
    52741 aagtacaaaa atatcatgat gatgttgata tatatgttgc atcagttgac gaaagattaa
    52801 atgaacatgg atatataatt cctggtcttg gtgatgctgg agatagattg tttggaacta
    52861 aataaaaata aataactata aatatttatt agggactttc ttatatagtg tatataattt
    52921 tatgtaagaa ggttcttttt tatatatttt ctcaaaatag gaattattga attgataaat
    52981 ttctcgaatt gagaataaaa agtttttacc aattaaaaaa gattgcttat tttacaggtt
    53041 tttatagaat agtagtctat tttaatatgg catgttagtt gcttacgatt aagtttgtag
    53101 gtattaagtg tattagttaa attgagaata gaatattaat aatattattg ggagtggtaa
    53161 tacatgaaga attgtatagt agtaagaggg gctggagatt tagcaacagc agttattcat
    53221 aaattacaca acagtggttt taaagtagtt gcgttagaaa tagaagagcc attagctata
    53281 agaaggcaaa tttctttttg cgaagctgtt tatgaaaaag aagtaactgt tgaaggtgta
    53341 gaatgcatat tttgcaatac tgaaaaagaa atagaagaag ttttatctca acataaagta
    53401 gccttgatta tagacccaaa aggagaaaaa ttatctaagt tgaatccaat cgttttggtt
    53461 gatggaatac ttgctaagaa aaacttaggt acaactaaag atatggcacc acttacaata
    53521 gctttaggtc ctggattttg tgcaggagaa gatgtagatg ttgttataga gacaatgaga
    53581 ggtcataatc taggaagaat aataactaat ggtagagcag aaaagaatac aggagtacca
    53641 ggagcaataa aaggtttttc taaagagaga gtaatgcatt cgcagtatga tggtataatg
    53701 gaaaatgtat gtaagatagg cgatatagtt gaaaagggag acctactttg ctatattaat
    53761 aattatgaaa aaaaacatga agttagagca acaatatctg gagtccttag aggtttatta
    53821 aaggatggag caaaggtaaa caaaaattta aaaatactag acatagaccc tagaaaagaa
    53881 gaagttaaga atgcttttac aatatcagat aaagctagat gtatagctgg aggggtttta
    53941 gaagccatac taaataatgg gatttatccc caaaattaat ttatattaga taaagctatt
    54001 aatattttat taagatgata taataaattt aatagattaa agagagcata tgacgggtat
    54061 ttaaaattta aatggtaaaa atgctctttt tttattggac attcaattaa catgtgtata
    54121 taatataaaa ataagtattt atttaggttg aaatctaata aatatataat accagtacat
    54181 aagcaaagag gagaaaagta aattatgaga ccatcatggg atgaatactt catggaaatt
    54241 gcagaagtag tgaaaaagcg ttcaacttgt attagaagac aagtaggagc tgtaattgta
    54301 agagacaaac aaatattaac aactggatat aatggttctc cgagaaatct tgaacactgt
    54361 gaaaatattg gatgtaagag gcaggaatta aatattccct caggagaaag acatgaatta
    54421 tgcagagcat tacatgctga acaaaatgcg ataatacaag ctgctcataa tggtattagt
    54481 gtagatgggg caactttgta tgttactaca agaccttgtg ttttgtgtgc aaaaatgtgc
    54541 ataaatgcag gtatattgaa aattgtttat gaaggtgatt atccagatga catgtctaca
    54601 gaattattaa aagaagcagg aatagaactc ataaaatttt agtggataaa atttatatga
    54661 caataaatgt caaattatga cactgaaata caatggtatc aaatgattaa aagaaagtat
    54721 ttgaaaaatc atcagattag tagtaagata tattttataa ttaaataaat ttatgagata
    54781 gattaattac atgttattta ttttataata atgagggata ctatttatga gtaaaaaaaa
    54841 tacacataca gaaattgcaa agttgtttag tttaataagt caaataggat tgatgattgt
    54901 aatatcaata ttaggatgta ctttcttagg aaaattttta gatagtaaat tgaatactac
    54961 tccagttctg acaataatat ttttattgtt gggtgttgga ggtgctttta gtggtgttta
    55021 taaaactcta atagtatata cacgaaggaa gtgatatatt ggatataaaa ttacttaacc
    55081 aggtaaggtc agtaacaaaa ggtatagtaa tatttgatgt agttttaatt gtgctaatgt
    55141 tgataacatc aacattaagt aaaccagtat tattaggact tatatttggc tctataatag
    55201 ctatgttaaa ttttagatta ttggcgatat cactggaaaa atcagtgaca tttcctgctg
    55261 gaaaggcaca agcttatgca tcagcacaat atgctattag attatttata atggcagttg
    55321 ttctaattgc atcagctacg gtaccacatt taaatataat cggaacagca ttgggattat
    55381 tgagcacaaa gtttgtcata ttatcaaaaa actttataaa taaattaaag aggaaggagg
    55441 cataagtatg aatcaagcgc attattatct cataggaaac tttaagatta gtgaaacaat
    55501 actagtaagt tggggaataa tggccttttt agcgatagca tcttattttt tgactagaaa
    55561 cttaaaaaaa gttccaacta gtaaagttca aatattctta gaatttgcag taggtggtct
    55621 tgctaaaatg gttacagatg ttatgggtaa aaatacagta aaaagaatgc catatatgat
    55681 accatacata ggaagcttgt ttttattttt cgcatgttca aatttaagcc cattacttgg
    55741 attaaggtca ccaacaaccg acttagacac aactgctgca tgggcaatga ttacattttt
    55801 tatgatttat tttgcaggag ttaaatttaa ggggttatca tactttaaag aattggctca
    55861 accacttgcc ataatgttac caatcaatat tataggtgag ttagctagac caatatcatt
    55921 aagtttccgt ccatttggga atattttagg tggaacaata ataatgggtc tgttttatca
    55981 acttttaggt tttatatcta gtttaatacc aggattatca ataccaattg gacagatatt
    56041 aataccagtc ccattacact tgtactttga cttatttgca ggattgttac aagcatatat
    56101 atttataatg ttaacaatgg tatttatatc aagtgcagga gagttagatt aagaaatcaa
    56161 cttatattaa gataaaaata aaataattta aaaaattaaa gttttaagga ggaaataatt
    56221 atggaaacag caatagtagc ggcagcatca gcaataggag cagggatagc agttgcaaca
    56281 ggtataggtg caggtatagg tcaaggtata gcagcagcta aagctgcaga agcagtagga
    56341 aaccaaccag aagctaaagg tgatataaca tcaactttat tactaggagt tgctatagcg
    56401 gagtcttcag caatatatgg tttggttata tcaataatac ttctattcgt aaatccattc
    56461 tttaaatact taggaatgta gatttagtcc ttaaatattt tgagataggt ttataggtaa
    56521 atcatctcaa acttataatc atgagatgaa ttttatcatc tcgtgattat aagtagttat
    56581 gatttatgga aaggaggata attatggaga aagcactagt aggtataacg tgggaatttg
    56641 tgttccaaat tgttaatact tttataatat ttttacttct gagaaagctt ttattcaagc
    56701 cagtattaaa tataattgaa tcaagagaaa atgatattaa aagtgatttg gcagaaggcg
    56761 aaaaagcaaa aaatgaaggt ttagctctga aaaaagaata tgagtcaaaa atcaattttg
    56821 ccaaggatga aggtcaagaa atcataaaac aagcaactat aagagcagag cagaaatcag
    56881 atgatatagt aaatactgca aagaaagatg ctcttgatat aaaagaaaaa gcaaacaaag
    56941 atatagaaca agaaagacaa aaggttataa atgaaatcaa aaatgatata tctaacatag
    57001 cactgcttgc agcatctaaa gttattgaaa aagatttaga taaatctaaa catgaagaat
    57061 tgatagaaaa ctttataaaa gaggtagggg aagctaaatg ataaatgtaa tagcaaatag
    57121 atatgccgaa gccctatttc aattaggaga agaagaaaat tcaactgatg tcttattcaa
    57181 agaactagaa aaagtagtag atatgatgac taaagttagt aaagatttct acaaagtttt
    57241 aaaatcacca ttagtttcaa aatcagaaaa gaaaaacttg gtagaaatta tttttagtaa
    57301 ggaagtaagt tctaacataa aaaacttttt aaaagtttta gtagataaag atagaatatc
    57361 atatttagaa gatatcgaat tagcttataa agaattatta aataagaaaa acaatgtaat
    57421 tgatggagta gcaataagtg ctattccaat gagtgaaaca gatattaaag aacttgaagt
    57481 caaactttca aataaataca acaaaaatgt tactatagaa aatgttgttg acaagactat
    57541 actaggggga gttctagtta gaattggaaa tgaacaaata gatggcactg tgaaaactcg
    57601 tcttgataag atgaaagaaa agctatctga agtaatatct taagaatggc ggtgaaccca
    57661 tgaacttaaa acctgaagaa ataagttcta taattaaaca gcaaataaaa aattatgaga
    57721 ataaagttga gttgacagat acaggtagtg ttttaacagt tggggatggt atagcgagtg
    57781 tatatggatt agaaaaagct atgtctggtg agttgctaga gttcccaggt gaaatatatg
    57841 gtatggcact taacttggaa gaagaagtag ttggagctgt tatccttgga gatgacagtg
    57901 aaataaaaga aggcgatata gttaagagaa ctggaagaat agttgaagtt ccagttggag
    57961 aagctttaat aggtagagtt gtaaactcac taggtcagcc aatagatgga aaaggaccta
    58021 tagcgtacac aaaaactaga ccagttgaat ctgaggcacc tggtataata gatagaaggt
    58081 ctgtttatga accattacaa acaggaatca aatcaataga ttcaatgata ccaattggta
    58141 gaggtcaaag agagttaata ataggtgaca gacaaactgg taaaacatct atagttattg
    58201 acactatact aaatcaaaag ggaaaagatg ttatttgtat atatgtagca attgggcaaa
    58261 aacgttctac tatagctcaa ttagttagct ctttagaaaa aggtggagct ttggattata
    58321 caatagtagt ttcagcaaca gcatctgaat cagcaccact tcaatatata gcaccatatg
    58381 ctggagcagc tatgggtgaa gaatttatgt acaatggaaa acatgtactt atagtgtatg
    58441 atgatttaag taaacaagct gttgcatata gagaaatgtc attgttactt agaagaccac
    58501 caggacgtga ggcatatcct ggagatgtat tctatttaca ttcaagatta cttgaaagag
    58561 cagctaagtt atctgatgaa ttgggtggag gttcaatgac tgccttgcct ataatagaaa
    58621 cacaagctgg tgacgtttct gcatatatac caacaaatgt tatatctata acagatggtc
    58681 aaatatactt acaaccagag ttattctatt caggtgtaag accagcagtt gaccctggta
    58741 tatcagtatc aagggttggt ggttctgcgc aaattaaagc aatgaaaaaa gttgcaggaa
    58801 cattaaaact tgcatattca caatatagag aacttgcagc attctcacaa tttggttctg
    58861 acttagatga agatactaaa aagagacttg ctcaaggtga aagaatcgtt gagatattaa
    58921 aacaaggtga gcatcaacca ataaaagttg aaaatcaagt tatgataata tatgctgtta
    58981 taaataatca tctagaagat attccgatag ataatatagc tagatttgaa tcagaattgt
    59041 atgcatttgt agataataat tatccagaaa tatcaagaaa aatattaggt ggagaagact
    59101 tcactcatga tttaacagat gcaataaatg aatttaaaga aaagtttgtt gttgaagtat
    59161 aatccttttt aaaaaaggag gtaaattaat tggcaggagc tggaatcaaa gcaatcaaaa
    59221 cacgtatggg aactgttgaa agtacaaagc aaataactaa agctatggaa ttagttgcat
    59281 cttctaaact tagaaaagca aaggaaaagg cagaagattc aagagaatat tttaatactt
    59341 tatacaatac agtaaaagat attgcccaaa caacacgtgg aattagaaat gaatttttaa
    59401 aagaaagaga agtaaaaaat aagtgctatg tagtagtagc tggagacaga ggacttgcag
    59461 gcggatacaa ttccaatgta ttaaaggaat ctgtctctca tatgaaaggt aaaaaggaga
    59521 cagtgattac tgttggaaag aaagcgcatg aatttttttc taaaagaaac tacaatatag
    59581 taaaagatat aaatagtgtt gaagcttgtg gatatgaaga tgcgacagaa atttcacaaa
    59641 ctattattga cctgtataaa aaaggtgaag tagatgaggt ttatattgct tatacaaaat
    59701 ttaaatctgc tttatcacaa gaagttgaaa ttttgaaatt gcttccatta gtctttgaaa
    59761 aagatacaga cgaacaagga atggcagtaa aagaagaaaa acaaaataga tcaaaagtac
    59821 aatatatacc atcagcagaa gcagttttaa gttatattgt acctcagttt atatcaggta
    59881 cagtatatgg tggaattttg gaatcttttg catcagagca aggtgctaga agaactgcga
    59941 tggaatcagc cacagacaat gcgaatgaaa tgctatctaa tttagagtta agttataaca
    60001 gagcaagaca gtcagctgta actcaagaaa taacagagat agttggtgga gttgaagctc
    60061 taaaataggg aggttaggaa atggcaaatg taggtaaagt agtacagatt gtaggagctg
    60121 tactagatgt taagtttgat agtgaacaaa gcctacctaa cttattaaat gcgttagtaa
    60181 taaagttagg agataaagaa atagttgcag aagttgctca acatataggt gatgatactg
    60241 taagatgtat agctatgagt gcaacagacg gacttgtaag aggtatggaa gttgtagata
    60301 caggaggacc aataagtgtc cctgttggag atgagacttt agggagaata ttcaatgttc
    60361 ttggaaaacc agttgatggg aaaccagctc caaaatctgc acctaaatta cctatacata
    60421 gaccagcacc ggcatatgat gaactagaaa ctacagcaga gatactagaa actggtatta
    60481 aggtagttga cttactagct ccatatctaa aaggtggaaa aataggtcta tttggaggag
    60541 caggagtagg aaagacagtt ctaatacaag agcttataaa caatattgct aagcaacatg
    60601 gtggtatttc tgtattttca ggcgtaggag aaagaacaag agaaggtaac gacctttatg
    60661 gcgaaatgag tgagtctgga gttataaata aaacagctct agtatttggt caaatgaatg
    60721 aaccacctgg agcgagaatg agagttgctt taactggact tacaatggca gagcatttta
    60781 gagatgagca aggacaagac gttttacttt tcgttgataa tatattccgt ttcacacaag
    60841 ctggttcaga agtttcagca cttctaggac gtatgccatc agctgttggt tatcagccaa
    60901 cattagctac tgaaatgggt gcacttcaag agagaataac atcaactaag aaaggttcaa
    60961 taacatctgt tcaagcagta tatgtaccag cagatgactt aactgaccca gcaccagcta
    61021 caacattctc tcacttagat gcaaaaacag ttttatcaag acaaatatca tcactaggta
    61081 tatatccagc agttgaccct cttgagtcta catcaaggat tcttgaccct agtatagtag
    61141 gaaaagaaca ttatgaagta gctagaggtg tacaatcaat acttcaaaga tataaagaac
    61201 ttcaagatat aattgctata cttggtatgg atgaactatc tgatgaagat aagttaatag
    61261 ttgcacgtgc tagaaagata caaagattct tatctcaatc attcacagtt gctgagcaat
    61321 ttacaggaaa tccaggtcaa tatgttcctg ttaaagagac tgtaagagga tttaaagaaa
    61381 ttcttgaagg gaaacatgat gacttaccag agtcagcatt cttatttgta ggaacaatag
    61441 aagacgctgt tagaaaagcg aagggaagta tgtagtcatg gccagtgaat tttcattgaa
    61501 agttgtaact ccaagtaata ctttttatga tggagatgtt gaaatgatag tagttagaac
    61561 tacaaaagga gacaaagggg tgttgaaagg tcatatacca tttgtttcag gtatacctgt
    61621 aggaacttta aagataaaaa aagagggaag ttttaaagag gcgaaaatag cagaaggatt
    61681 catcaatgta actcaagata aaactgtcat attgacagaa tctgctgagt ggatttaata
    61741 atttaattta gaatataaaa agtcattttg accaaaaaaa cttttgggta aaatggcttt
    61801 tttgtatttt gatgttaata aaataacgat aaaattgtaa atttttagtg ataataaatt
    61861 cacaaaatgt ttaaatttac tgcaaaatta tgttgtaatt aataagataa agaagattta
    61921 gatagctttt atgtaagtga aaaagtcttc ctataagaaa tacatataaa atttttataa
    61981 aatttaaaaa atttgcttag tt
//
LOCUS       DAEPXF010000003        59124 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain HB27
            SAMN13376389-rid12479423.denovo.003, whole genome shotgun sequence.
ACCESSION   DAEPXF010000003 DAEPXF010000000
VERSION     DAEPXF010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13376389
            Sequence Read Archive: SRR10518432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 59124)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 59124)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001069624.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001069624.1
            Genome Coverage       :: 315x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/15/2021 01:54:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,165
            CDSs (total)                      :: 4,076
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 89
            rRNAs                             :: 10, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 10 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 19 of 69
            Pseudo Genes (incomplete)         :: 44 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 10 of 69
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..59124
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13376389-rid12479423.denovo.003"
                     /strain="HB27"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="China: Hebei"
                     /collection_date="2017"
     gene            <1..1243
                     /locus_tag="KRL67_000154"
     CDS             <1..1243
                     /locus_tag="KRL67_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="bifunctional diguanylate
                     cyclase/phosphodiesterase"
                     /protein_id="HBH1521068.1"
                     /translation="KVTYIGNQNKFYRECSKYLLDKPSLNYIIVYFDINNFKMINDTF
                     GYEFGDNLLITIAKALKEELTEGEVYARLSSDYFAIFCDYKNGRNKIIRKLDSIRNNI
                     ESNLSIVFEISLCVGIYFVEEGEVDIQKAVNKANMARSVAKGKNINYAIYNEDVRNKL
                     SEESMILDDIKIALVKNQFEVYYQPKFSLVNGEMIGSEALIRWNHPEHGFISPAVFIP
                     IAEKSKLILKIGRFVFERVCTDLSEWKKQGKKIVPVSVNLSRVELYQPDIVKFINKTI
                     QMYNLSSDLIEIEITETVAINELNILKNILNELRKYGFSISMDDFGTGYSSISCLRDM
                     PIDILKLDKSFLDGIEHDERSRNIAKSIVSLAKSLDLVVIIEGVESKEQAELMKQFGC
                     DLVQGFYFARPMPAKNFLDLL"
     gene            1497..2159
                     /locus_tag="KRL67_000155"
     CDS             1497..2159
                     /locus_tag="KRL67_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH1521069.1"
                     /translation="MIDVSIDDKLKERWPNAKLGCIQAKVAVNKSSEKLINEINEFCD
                     TINQSLKIEDITKLDKIKDARNAYKELGKNPSRYRTSSEALVRRIIQGKGLYTINNIV
                     EINNLISIKSLYPVGTYNVSKLHSPVCFTVGDEGEQYKGIGKELINIENLPILSDSVG
                     KFGSPTSDSERAMITEDANEILICIFSFSQESDIEEYFGYAKELLKEYADGKDIETKI
                     IK"
     gene            2529..3938
                     /locus_tag="KRL67_000156"
     CDS             2529..3938
                     /locus_tag="KRL67_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase/peptidoglycan binding
                     protein"
                     /protein_id="HBH1521070.1"
                     /translation="MIDGKEVKQESGQKNLKFKIAIVVGVLFVAYIGMAIFFRNHFYF
                     GTTINGVKASGKTVEQVNDLLTSSTNSYTLNLEERNNKKEQIKAKDIDLKFTPDDKVQ
                     KIKDSQSAIGWIGGIFKSKEYNNMVNLSYNKDLLEKRFNALSCFDSKNVVEPKSAYPK
                     YDSTKNAYVIVPEVLGNKVKKDDFYKLVQNSINSGNTSISLDESKVYEAPNNTKDSEK
                     LKQAIKTLDKYVGVVVTYDFSDRKETLDGSEINKWLKVDSEKNYDITFDNDAMVEYTR
                     GLSKKYSTFGDSRPFKTATGKDITISGGIYGWLIDKKKEAEALVDVVKAGKNVTREPI
                     YIQKAVSRNRDDIGNTYVEISLSGQHMWFFKNGEMLVSTDVVTGDLARGFATPSGIYP
                     LNYKARNVSLTGQGYSSPVSYWLPFNGNIGIHDATWRNSFGGGIYKSSGSHGCVNTPF
                     SKAKKIYENIEPGTPIILY"
     gene            4053..4328
                     /locus_tag="KRL67_000157"
     CDS             4053..4328
                     /locus_tag="KRL67_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif11-like leader peptide family natural product
                     precursor"
                     /protein_id="HBH1521071.1"
                     /translation="MNEKLTKILEEAKNDDEFRKELIKTREQRDVMDSFCKVVTSRGY
                     DITVGELFSIGEEYTSNLLKSVNGGAAYPIEDWSDWYEDFFIMLSNI"
     gene            4957..5268
                     /locus_tag="KRL67_000158"
     CDS             4957..5268
                     /locus_tag="KRL67_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH1521072.1"
                     /translation="MAFEIIKSIVEAEQTADSIKVKAVTDAESIRADAVNKCESIFAD
                     VKKQAKLMEETLIEKAVTDSRAEVDKILANAKSECLKIEKTAEERKSKAIEAVIGKVV
                     R"
     gene            5270..7195
                     /locus_tag="KRL67_000159"
     CDS             5270..7195
                     /locus_tag="KRL67_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit I"
                     /protein_id="HBH1521073.1"
                     /translation="MAIVNMNKISIVGLEAQKSQILKLLMNRGFVQIDDSAFLTEEEE
                     LKNYLVKDSEESEVIMLEQKMSHVNQAINIVSSLVKQKKSLLAPKREFNKIDSEKEGQ
                     VYAEALELNNLHKEIGTIRSNINLLNNNKSMLEPWVDFDIPLENMETKYTKTILGTVP
                     ISANIDALRVKLEDEIPESVIGIVNSDKLMNYVYLITLKETFDDVLEVLKEFNFSVVS
                     LPNEEGTPLQAIKKYNEMIIRQENVSKDKTEEIKRHADSIHMLENLYDFYTIERDQKK
                     IIERLVKTKTTFCLNGWLPSKRADELIKEITEKFDCCVQTEEGSKEEGFPILLENNKL
                     VTPFESVTNMYSYPSTKDIDPNTILTFFFVVFFGMMLSDAAYGLIIAVVCGFVVYKLK
                     IQKGEGNLIKLIGICGVSTTVWGLIFGGILGDLIPIKALINPLEDVMTLMGMSLLFGI
                     IHIYVGLGIKAYTLIRSGKVVDAIFDIGFWYLCITGVCLLIIPFVAGDIGVFSEVGKY
                     LAIIGAIGLVLTQGRSFKGIPVKLFKGFSSLYGITSYFADILSYTRIMALCLTTGVIA
                     QVINLLGAIAGPILAVVIGVVGHTINLLINALGAYVHTSRLQYVEFFNKFYEGGGVPF
                     VPFKYKTKYTSINKKEM"
     gene            7198..7689
                     /locus_tag="KRL67_000160"
     CDS             7198..7689
                     /locus_tag="KRL67_000160"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit K"
                     /protein_id="HBH1521074.1"
                     /translation="MEFLNALGNGQFLAISGAAIAALFAGMGSAKGTSIAGQAAAGVV
                     TEDPSKFGQLLVLQLLPGTQGLYGFIIAFLILSKAGIIGGDAIPTSAQGLQLLMAGLP
                     IGLVGLVSGIAQGKAAAAGVGIVAKRPEELGKAITFAAIVETYALLGLLVSFLAYNGI
                     AIG"
     gene            7741..8304
                     /locus_tag="KRL67_000161"
     CDS             7741..8304
                     /locus_tag="KRL67_000161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit E"
                     /protein_id="HBH1521075.1"
                     /translation="MGNEQKMIDRIIADAKQEAQEILDKAKSEADLKVNSANEKAEKE
                     MASYTKLAEAEAEKAASKEISGAYMEAKKQILSKKQEILEEVILEAKNKLLNLKDNEY
                     EEIILNMIEKSNCTDDSEIVLSKKDKKTLKDVLSKKGIKVSDETRDITGGFIVKKGDI
                     EYNYSFEAIIAVEHEYIEQIAAEILFN"
     gene            8319..9296
                     /locus_tag="KRL67_000162"
     CDS             8319..9296
                     /locus_tag="KRL67_000162"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit C"
                     /protein_id="HBH1521076.1"
                     /translation="MAEEKTYPYAVARIRVLEKQLLNRQMFIQMAEAKSPEDSLRIIA
                     EAGYADTSNNNIHNFENILTQELSKTYEILKSLAPEEKFVDVFLYKNDYHNLKVLIKE
                     EISGVDGEKYLIDGGTIPLIKLRESLANRSFSDLPKIMSSAILEAFDVYSKTQNGQMV
                     DIVLDKATFTSMKETAKESQNKFVIDYVMKVCDLTNLKSFIRVKNMKKDFDMFMNVFV
                     SGGSLDKEKFLEAFSSDTPASCFKSTSYSDVCKNGMDSGFTVFEKLCDDYLMEYVRGA
                     KFKPLTLEPLIAYLYAKESEIKTIRIILTSKLNNIDADTIKERLRDAYV"
     gene            9283..9606
                     /locus_tag="KRL67_000163"
     CDS             9283..9606
                     /locus_tag="KRL67_000163"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit F"
                     /protein_id="HBH1521077.1"
                     /translation="MLMYKVGVVGDKDSIMGFLALGIDIFPAYDSDEIKKSIHKLVED
                     EYAIIYITEQASLLAKESIAKYKDYQLPAIIVIPGIGGSMGLGMNEVRESSKRAIGAD
                     ILFKE"
     gene            9723..11501
                     /locus_tag="KRL67_000164"
     CDS             9723..11501
                     /locus_tag="KRL67_000164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HBH1521078.1"
                     /translation="MKTGTIVKVSGPLVVAEGMRDANMFDVVRVSDKHLIGEIIEMHG
                     DKASIQVYEETSGLGPGEEVVSLGMPMSVELGPGLISTIYDGIQRPLEKMYDISGTNI
                     TKGVEVSSLDRTTKWEFKPKKSVGDKVVAGDIIGGVQETAVVECKIMVPYGVKGTIKE
                     IYSGEFTVEDTVCVITDEKGNDIPVTMMQKWPVRKERPYREKKTPDSLLVTGQRVIDT
                     FFPITKGGVAAIPGPFGSGKTVTQHQLAKWADADIVVYIGCGERGNEMTDVLLEFPEL
                     KDPRTGESLMQRTVLIANTSDMPVAAREASIYTGITIAEYFRDMGYSVALMADSTSRW
                     AEALREMSGRLEEMPGEEGYPAYLGSRLAQFYERAGKVNCLGMDEREGTLTAIGAVSP
                     PGGDTSEPVSQATLRIVKVFWGLDASLAYKRHFPAINWLTSYSLYQEKVDVWADKNVN
                     DNFSAQRAQAMKLLQVESELQEIVQLVGVDSLSDGDRLILEVTRSIREDFLHQNSFHE
                     VDTYTSLHKQYRMMGLILKFYKSAQDAIKQNVTINDIIKLSVLEKIGRAKYVEEGDID
                     AAYDSIEDEIDNELADLIKKRGAEEL"
     gene            11498..12871
                     /locus_tag="KRL67_000165"
     CDS             11498..12871
                     /locus_tag="KRL67_000165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HBH1521079.1"
                     /translation="MIKEYKSIQEVAGPLMLINGVEGVKFDELGEIELSNGEIRRCKV
                     LEVNGDTALVQLFESSTGINLAESKVRFLGKSLDFGVSPDILGRVFSGMGRPIDGGPE
                     IIPDKRLDINGAPINPAARDYPAEFIQTGVSAIDGLNTLVRGQKLPIFSGSGLPHANL
                     AVQIARQAKVRGTDSKFAVVFAAVGITFEDAEFFISDFKATGAIDRSVVFVNLANDPA
                     VERVSTPRMALTAAEYLAFEQDMHVLVIITDITNYAEALREVSAAKKEVPGRRGYPGY
                     LYTDLATMYERAGKMKGHEGSITMIPILTMPEDDKTHPIPDLTGYITEGQIILSRELY
                     KKDIQPPIDVLPSLSRLKDKGIGKGKTREDHADTMNQLFAAYSRGKDAKELMAILGES
                     ALSEIDLMYAKFADEFEKEYVSQGFRTDRTIEQTLEIGWKLLSMLPKSELKRIRDEYF
                     EKYMPKE"
     gene            12877..13545
                     /locus_tag="KRL67_000166"
     CDS             12877..13545
                     /locus_tag="KRL67_000166"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HBH1521080.1"
                     /translation="MARLNINPTRMEMTRLKKLLKTATRGHKLLKDKLDELMKQFLEI
                     VRENKRLREEAENALDTAYKNFIIARAVMSQEYLGSALMMPKQSVSVDVSTRNIMSVD
                     VPVFDFKTENNQSDIYPYGLAFTSGELDSAMEAFSDAMQPLLRLAESEKSAQLLAQEI
                     EKTRRRVNALENVMIPNYIETIKYIAMKLEENERASTTRLMKVKDMVLKKALEEKKKN
                     DLVV"
     gene            13822..14871
                     /locus_tag="KRL67_000167"
     CDS             13822..14871
                     /locus_tag="KRL67_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative 2-aminoethylphosphonate ABC transporter
                     substrate-binding protein"
                     /protein_id="HBH1521081.1"
                     /translation="MKEFIFTCLCIVVITFMTSCISTTPQTTQGKGNNKLTIYAALEE
                     EQIAYYMEGFREEYPNIDVEIVMDSHGVIASKLLAEKDNPQADIIWGLSAMNMVELKK
                     DGALEAYKPKNYDKVDKKFKDTEDNVSWVGMSVPETAIVVNYKELKKLGIDVPKSYED
                     LIKPEYKGLITMPNPASSGTGYLTVSGLIQMMGEKDAYNYMDKLHKNIATYTHSGSKP
                     AKLAGSGEYPIGITLGYRATQQLSSGEPIEVVFPKEGSGWDLEANALVKKDNIKKEAK
                     LFLDWAISDSAMDRYAHEVAVTSIKTDNPVPKGYSKQPFEQLIKEVDLNSAAKNRSTV
                     LKTWESKYGSKSESK"
     gene            14961..15077
                     /locus_tag="KRL67_000168"
                     /pseudo
     CDS             14961..15077
                     /locus_tag="KRL67_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728969.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="putative 2-aminoethylphosphonate ABC transporter
                     ATP-binding protein"
     gene            complement(15100..16458)
                     /locus_tag="KRL67_000169"
     CDS             complement(15100..16458)
                     /locus_tag="KRL67_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HBH1521082.1"
                     /translation="MLRVALYIRVSTEEQALNGDSIRTQIEALEQYSKENDFNIVGKY
                     IDEGCSATNLKRPNLQRLLRDVEKDKVDLVLMTKIDRLSRGVKNYYKIMETLEKHKCD
                     WKTILENYDSSTAAGRLHINIMLSVAENEAAQTSERIKFVFQDKLRRKEVISGTIPIG
                     YKIENKHLVIDKEKKYIVKAIFDEYEKSGSVRTLIETINNLHGELYSYNKIKNILRNE
                     LYIGIYNKRGFYVEDYCEPIISKKQFKQIQRILEKNKKTTPNKNIHYHIFSGLLKCKE
                     CGYTLKGNSSNVGEKLYLSYRCSTFYLNKNCVHNVTHNEKHIENYLLTNLKPQLHKHM
                     VKLEAQNEKIRRNKKSNKKDEKKKIMKKLDKIKDLYLEDLIDKETYRKDYEKLQSQLD
                     NITEEQESQIIDTSHIKKFLDIDINEMYSDLSRVERRRFWLSIIDYIEIDNNKNITIN
                     FI"
     gene            complement(16523..16996)
                     /locus_tag="KRL67_000170"
     CDS             complement(16523..16996)
                     /locus_tag="KRL67_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HBH1521083.1"
                     /translation="MNSYEKLLSEADDNNIIVREVPLISNSHGLYKNNRIALNKNTLN
                     NISEKACVLAEELGHHYTSYGNILDLNKVENSKQEYKARLMAYNKLIGLKGIIDSFNA
                     GCKTITEMAEYLDVTEKFLNEALECYKSKYGFSATLDNYVIFFEPRFSIMNANFL"
     gene            complement(17014..17469)
                     /locus_tag="KRL67_000171"
     CDS             complement(17014..17469)
                     /locus_tag="KRL67_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBH1521084.1"
                     /translation="MNTENDLKMLEITNRIKNKRLELNMSYQDLANKTGLSKSTLQRY
                     ETGAIKNIPLDKLEILAHALDESPAFIMGWNEDCKQIKIKENKLLSSFNALNDIGQNE
                     AIKRVDELTQIGKYVNKNHIDTIAAHNEHLHEEGEIEKIYQDLDDMDNW"
     gene            17623..17844
                     /locus_tag="KRL67_000172"
     CDS             17623..17844
                     /locus_tag="KRL67_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBH1521085.1"
                     /translation="MNISKLKGKMVEKKYTQKKLAEELGMTVQALNAKLNNRSQFKIE
                     EAVKISNILQIEEPGEIFFDNYIPKMQRN"
     gene            17892..18686
                     /locus_tag="KRL67_000173"
     CDS             17892..18686
                     /locus_tag="KRL67_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ORF6N domain-containing protein"
                     /protein_id="HBH1521086.1"
                     /translation="MSNNLQVIERNNKRVLTTQQLADVYETDSKNISNNFNNNKDRFI
                     EGKHYFLLQGDDLKNFKGIHTEYENLKFASKMYLWTERGANRHCKILDTDKAWEQFDN
                     LEETYFKVKQQKPTCIEDVLIESLKEMKDLRLQVNQANNIALEAKTEVKTIKEVVSLN
                     ATDWRKDTQQLIAKIAKKQGGFEHINMLRRESYELLNNRFGVDLNRRLINKRRKMAEE
                     GVSESKREKVNNLDVIQDDKKLIEGYVAIVKDMALKYGISSDLSKN"
     gene            18752..18940
                     /locus_tag="KRL67_000174"
     CDS             18752..18940
                     /locus_tag="KRL67_000174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH1521087.1"
                     /translation="MGKKSEYTVTTVGEPNLDALGETLAKILTEKFGVLITHTYIENY
                     YVDKERGGDVDGAIESEL"
     gene            18915..19142
                     /locus_tag="KRL67_000175"
     CDS             18915..19142
                     /locus_tag="KRL67_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH1521088.1"
                     /translation="MELLKVNFNLEKLKIGNVVSVSSKRFGFNINCIVVVATEEELNL
                     AYYSKDKGCIEYQALIPEDIRYGDYILERLG"
     gene            19242..19559
                     /locus_tag="KRL67_000176"
     CDS             19242..19559
                     /locus_tag="KRL67_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH1521089.1"
                     /translation="MESLKIVKQYVEGQLNLSSLEIDKNKETYEILKNKSSRDMLDDI
                     NLNDALREVTVNERLKIFAESLLELLDTQIKIKESEESEDYKRLCMYLDEFGRDRPID
                     VQI"
     gene            19645..20154
                     /locus_tag="KRL67_000177"
     CDS             19645..20154
                     /locus_tag="KRL67_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siphovirus Gp157 family protein"
                     /protein_id="HBH1521090.1"
                     /translation="MSTLYELTTDLLEIEEGLTETTGNEAEKLEEIKEIIKQEIQNKN
                     TRIVSVILNIDSDINSIDSEIKRLQELKKVKKNTLDRLKSNIKDCMELLGTKKVETFL
                     GNISIRKSAGSLVIEDEEKIPAIYKTVEQVIKVDKNAIKDFIKKGHEIEGCRIEYGTT
                     LTIPKAKKE"
     gene            20164..20748
                     /locus_tag="KRL67_000178"
     CDS             20164..20748
                     /locus_tag="KRL67_000178"
                     /inference="COORDINATES: protein motif:HMM:NF016302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ERF family protein"
                     /protein_id="HBH1521091.1"
                     /translation="MESNNIYIKLMDVRVKFSKLNLKKSGENKFANFKYFELADFLPQ
                     ATGLLEEAKLCPIVTFTNEYATLTLINGENPSEQIVFTSPMRDLQLKGSNELQALGGI
                     ETYQTRYLYIQLLNITESDTFDATSGKNEAKSNSNNRILTDKQLSRLYAIASNANVDK
                     ESLKEKVFKRFGKEIKDLTKQEYDTICNAYENKQ"
     gene            20763..21644
                     /locus_tag="KRL67_000179"
     CDS             20763..21644
                     /locus_tag="KRL67_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016056364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HBH1521092.1"
                     /translation="MGIIRVSKDKDNPYVVLNKTCLEDVKLSWQAKGLHSYLISKPDH
                     WKIYVNDLYKRSKNGRDATANILRELIENGYITRTPCRDSNTNKMLGGYDYQVYEIPL
                     ENPQKLKSRKTDFPETGFPGNRVSRKPENTEVVSNDFKVNNDITTIVINEQPQQDETT
                     YIKKYFEKYIGVITPNNFIELMSYLDDGMEADVIIRAIDEAIANGVKNYKYVKTILNN
                     WIEAGVKTVLELTEYQNEFERKKKSKQEKKQSNSKTVNTHNVNKNKFANFNQTFTQYE
                     EKELDEIIKKSQKEKFK"
     gene            21703..21876
                     /locus_tag="KRL67_000180"
     CDS             21703..21876
                     /locus_tag="KRL67_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="resolvase"
                     /protein_id="HBH1521093.1"
                     /translation="MARIYAQRSGSLNEQDRLELLRLLGKAGYTVKVAREKQNSKTTY
                     TYFVEYTEEQEEK"
     gene            21892..22299
                     /locus_tag="KRL67_000181"
     CDS             21892..22299
                     /locus_tag="KRL67_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HBH1521094.1"
                     /translation="MNTITLVGRLVADAELKYLPNSGTPKITFSMAVGRRFKDKNGNK
                     ITDFIQCEQLGKHVENLVQYLVKGKPIYAVGELNIYNYKDENGCWKSITKVNVNALEL
                     LSSKNDNNAKQEYVPPGLDPQGFQAIDDDDIPF"
     gene            22368..22619
                     /locus_tag="KRL67_000182"
     CDS             22368..22619
                     /locus_tag="KRL67_000182"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH1521095.1"
                     /translation="MRFEIGKTYKFDREKFIACNGIEHYKRCEELWIEDVEDVEFTVK
                     KTFDDGYVHYPNEFYFAFGIVPEWCVEVNEVGGIRDDNN"
     gene            22606..22857
                     /locus_tag="KRL67_000183"
     CDS             22606..22857
                     /locus_tag="KRL67_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH1521096.1"
                     /translation="MIIIRSQDRLDLMRVNRVEISNNRVYAMLEDDIRKIGEYESNER
                     AMQVLNEIQKFIENGVKKDYIDSCRVRHNQEKVFEMPVE"
     gene            22873..23226
                     /locus_tag="KRL67_000184"
     CDS             22873..23226
                     /locus_tag="KRL67_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016056367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH1521097.1"
                     /translation="MAKIWMDAGELLEKTIDIEDMFGCNLSKMRNKSKQKGLVGRTNS
                     KGQGRKSKKVECTNIITGESKIFVSAVEASKYLYFTELHITRLARTGKTTKNGWKVRY
                     IQEVSDGISKCGTSN"
     gene            23195..23344
                     /locus_tag="KRL67_000185"
     CDS             23195..23344
                     /locus_tag="KRL67_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH1521098.1"
                     /translation="MVLANVEQVIKLAEKILNKKKCSVNKAIDIAIKILSKYECEGMI
                     RNEFN"
     gene            23331..23780
                     /locus_tag="KRL67_000186"
     CDS             23331..23780
                     /locus_tag="KRL67_000186"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH1521099.1"
                     /translation="MSLIKYRGYDFENEKWIYSATIMWSDAVDCLFMLKENCEWQRVC
                     NVGVCSGEWARNNQEICEGDILKEYDNFHDTSQYGVVKRDVDSIKIYLEWHYLKKLEG
                     EWIEIINKTEIYHSRDYKVVGNEYENLEEVRKEFLERKEMLENEHLG"
     gene            23883..24059
                     /locus_tag="KRL67_000187"
     CDS             23883..24059
                     /locus_tag="KRL67_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH1521100.1"
                     /translation="MDDRLEMINASVNYIQMICESSNIAIITERGRVRILDLETKEKY
                     DLLKNKLEEMLEEI"
     gene            24173..24868
                     /locus_tag="KRL67_000188"
     CDS             24173..24868
                     /locus_tag="KRL67_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH1521101.1"
                     /translation="MDLNKIMNDALVEIEESGFVEETVKKELEETIKRAVNEIFGSYS
                     KFRKNIEQHLGENININLDELDIQKYNLLVANVVKEKVDATMKGQGIEHLKRNLDNML
                     VGLKKEYKMSELLEELKEDKYNLDEYPCDEDCITFILESNFNSEWARWIDFDECPNVS
                     RNNCEHSILLRNDGTIAAFRYQNREISSKDIMNGFGNFGDLLFKIYAHNSKIILDLGN
                     DVDDYDLTNGEDY"
     gene            24884..25105
                     /locus_tag="KRL67_000189"
     CDS             24884..25105
                     /locus_tag="KRL67_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH1521102.1"
                     /translation="MSKYVLRWQKGLLLDERKINYSCGSIENLKKKAELLAKDDKILF
                     ITIDKVEEVIKDTRSQKMAEYYCDGGIEI"
     gene            25102..26106
                     /locus_tag="KRL67_000190"
     CDS             25102..26106
                     /locus_tag="KRL67_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein"
                     /protein_id="HBH1521103.1"
                     /translation="MIIHKFIIHVLDKNSDTPILNDFEGRVSQDIEAFFQKKISKVSR
                     DNDIRTAVFNDYSNNLIKKCCEQIIYDESSFLNNSKEIAAYLFDVMKLNAILESCDLA
                     ICLYSQKDEKKVAILKLDYNNSYTHSISFEDDKFNIQMSKNEINIQETKTVKIAALVG
                     LSGMNDEYHLKVLDKDAEKEEANSKFVTEFLNATRVKDDKYRTKKFKDTVENWITNVL
                     GNDIKQAEDIRSILNYTLKEKHEIDIKDFVDKSIKDDELKNSFKEHMEEKGLDESFSI
                     DKKWVEKKLKKRNIKTDNGFEIKGNLTDFEDPMKYTVRQNQNGSIDIIIKNVNFYNEK
                     "
     gene            26117..26407
                     /locus_tag="KRL67_000191"
     CDS             26117..26407
                     /locus_tag="KRL67_000191"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH1521104.1"
                     /translation="MENKDNFVLNKLGPASKVMKKVEIDEKEIKSYLKELKECEEVKV
                     FIIRKDGIEIPIVFKDGNMNEWLRKEIEISFDRFKDEIAQVKKALIKVTGEN"
     gene            26427..26594
                     /locus_tag="KRL67_000192"
     CDS             26427..26594
                     /locus_tag="KRL67_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH1521105.1"
                     /translation="MTNKEMCKSNNLDEREVYKSFGKEICGSCKSNKGDCESKNCSEA
                     CKNWLEKVCKS"
     gene            26591..26944
                     /locus_tag="KRL67_000193"
     CDS             26591..26944
                     /locus_tag="KRL67_000193"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH1521106.1"
                     /translation="MTELGELIREIREEEGEYISDMAKRLNISCEDIYAINRGKRELN
                     KNEINNVVKKYELEGEHLYLLKNITHKDKLFDIACNECGSKNVAIGMFDVANDIIEFR
                     CRNCNMIDVVSVDFY"
     gene            27038..27376
                     /locus_tag="KRL67_000194"
     CDS             27038..27376
                     /locus_tag="KRL67_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor-like protein"
                     /protein_id="HBH1521107.1"
                     /translation="MANIYCENYNCKNYFEDMCMLERIEINNLKVCESYIEGKNELYK
                     LENGYTIHPKDLKMVKSKDYSVEVTHIPTGITVKCRSTNSILKNKNKCLEVLEEELIK
                     NSHLELEDLR"
     gene            27502..27909
                     /locus_tag="KRL67_000195"
     CDS             27502..27909
                     /locus_tag="KRL67_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HBH1521108.1"
                     /translation="MKVFLVIDGEPVGKERPRFNLATKRTYTPNKTRDYEGLIRWLYQ
                     SKVKYRFTGYIKMTIRCYYSIAKSNSKKVKEQKRNNVLRPSKKPDIDNVVKIIADSLN
                     EIAYKDDTQIVEVVASKYYSDNPRVEVILEDVI"
     gene            27924..28649
                     /locus_tag="KRL67_000196"
     CDS             27924..28649
                     /locus_tag="KRL67_000196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antA/AntB antirepressor family protein"
                     /protein_id="HBH1521109.1"
                     /translation="MNEIMTNENLRVVADDLVTVYETDTGEKIVFARELHNNLEVKRQ
                     FIDWIEDRIKQYGFKENEDYSVFHKNMKNSTGGRPSKEYVLKLDVAKELAMVQNNNKG
                     REIRRYFIRLEKLLNRTLSNSQLSQINTIVNESLLKMEVKHNIQIEQLKKECSEYYRP
                     TSKTKYDISSYIKERLGISKVNEEFELVKKRTLLVLGADKWEDIPKDVLLNSLNLIDE
                     SIRIIKSERKTSQISFFEKDNFC"
     gene            28761..29243
                     /locus_tag="KRL67_000197"
     CDS             28761..29243
                     /locus_tag="KRL67_000197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015994385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH1521110.1"
                     /translation="MNKKTLFQEVEGRLYNYKKLESQIRIKDIYIKKLESEFCACKAQ
                     SYEEKTGPTYNISSSVENEVIKREEDLNRLKEDKKTLQIEKETIECALTSLNSFETEF
                     FNEMYMNNEKINMDYMSNAMHIDRSHCFRIRKRIVCKIMDMLYPKIKEFELPIFSWKA
                     "
     gene            29848..30891
                     /locus_tag="KRL67_000198"
     CDS             29848..30891
                     /locus_tag="KRL67_000198"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH1521111.1"
                     /translation="MDKDIIKKVKFSEIDINDEFFKSLISDYINFEKWFLKKYNEEAY
                     ILKTNRGLQGFLYLKDEYDEDYSISPNFDKKRRLKIGTFKINSHGTVLGQRFISIILR
                     KMVEEDFKEAYVTLFEKQDGLIKLFERFGFSYWGKKDNGELVYVRDDADKYDIYENFP
                     RIKTTNVRSYLLGIYPKYHTELFPDSRLCTEKNHIIKDVSFTNTVEKVYLCNMSGIMN
                     LKRNDLLLIYRTKEGEKQGKFSSVATSICTVVETKHISSFSNYEDFMNYCGKGSIFSQ
                     DDLKYFWSKRKYPYLIKMIYNLPLNKRIIRKNLIEKIGLREKEYWGFFEIKQEQLRKI
                     LEMGEVNESFIID"
     gene            30869..31252
                     /locus_tag="KRL67_000199"
     CDS             30869..31252
                     /locus_tag="KRL67_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012450285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HBH1521112.1"
                     /translation="MKVLLSIKPEFVYEIINGTKKYEYRKSIFKREGISSIVVYATKP
                     YGKIVGEFEIDNIIQDKPSNIWKQTKEFSGITKKFFNEYFNGKDTGFAIKIKDFIKYE
                     VPLELNDFDKNIKVAPQSFCYLGED"
     gene            31254..31772
                     /locus_tag="KRL67_000200"
     CDS             31254..31772
                     /locus_tag="KRL67_000200"
                     /inference="COORDINATES: protein motif:HMM:NF024605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBH1521113.1"
                     /translation="MKYIFLAGVHAVGKTTLTKELEKKIDIVSYSISDLIRKSGNNIP
                     KNEKKTKDISNNQVLWIKELQNLNVENSTLILDGHFTLLNENEEVFPIDFNVFEKTNM
                     DKIILIKQNPKEIKRRLLNRDGNEYSLDKIEFHQSKEEEFAKKYAKTKQIPIYIYDSN
                     SDLESLVIFIDS"
     gene            31791..32030
                     /locus_tag="KRL67_000201"
     CDS             31791..32030
                     /locus_tag="KRL67_000201"
                     /inference="COORDINATES: protein motif:HMM:NF024363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3850 domain-containing protein"
                     /protein_id="HBH1521114.1"
                     /translation="MVHELKIDHEFFIPILENIKTFEIRKNDRNYKKGDIIILKELNE
                     DKASFTGRYIKAKITYISDYQQKDNYVVFSFEKEL"
     gene            32180..32932
                     /locus_tag="KRL67_000202"
     CDS             32180..32932
                     /locus_tag="KRL67_000202"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH1521115.1"
                     /translation="MKNFIKEVKLFILSNKILTLAFCIATIIFVSYEVTKDLPEIIPY
                     GEFIFNLFSQLSLAFMGCFVFYIMQVYIPERKRKKVTNNILANKIEYILIRMIEPAQC
                     ITNSILKKNLDINNMDKTKFASLYKKDYLDKGTKTIFRGGKEYTHKDYIKENICEINN
                     CIFEIYMTIGYNLDIDILDILSKIRDSKYNKIFIRESVLEPGVYDFKKINETPVTVTM
                     KEESFDKEKNRNEIIKEYYELYLSLRKYKRTL"
     gene            32981..33595
                     /locus_tag="KRL67_000203"
                     /pseudo
     CDS             32981..33595
                     /locus_tag="KRL67_000203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861025.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            33729..35084
                     /locus_tag="KRL67_000204"
     CDS             33729..35084
                     /locus_tag="KRL67_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014793000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBSX family phage terminase large subunit"
                     /protein_id="HBH1521116.1"
                     /translation="MALLRRKQKRQEKRFEFKPFSKKQLKLLNWWREGSKYKDYDIII
                     ADGAIRSGKTIAMICSFLMFTQTNFEVENFIIAGKTIGSLKKNVIEPMKQILNAWGWR
                     FEYNRSENFLVIGSNTYYMYDANNEASQDKLQGLTAAGALADEVALFPKNFVDQMIGR
                     CSVDGSKIFMNCNPAGPYHFIKTEFIDKVKEKLICYLHFTMDDNLSLSEKVKNKFKRM
                     FTGVFYKRYILGLWCQAEGVIYDMFNEKIHKVLTKLREYTEHYVSCDYGTQNATVFIL
                     WGKCKDVWYAVKEYYYDGRKEGKQNSDNKYYTELVNFLGNIHPKAIIIDPSAASFITL
                     IRDKGKYRVKKGNNDVLNGIRNVGTALNREMIKFNDCCSNIFKEFFSYVWDEKALEYG
                     EDKPVKVMDHAMDAIRYFVHTILFGGKEPNYDDEIYNKGLGLKKNNIQNQYNKKGGTV
                     F"
     gene            35084..36607
                     /locus_tag="KRL67_000205"
     CDS             35084..36607
                     /locus_tag="KRL67_000205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011099331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HBH1521117.1"
                     /translation="MNIKNILLNLDEKELRDRKHAERDFLFYLGECRNKTMGLLDDDF
                     LGQSWITFDNLDYTPSQIVDNKVKPLINKQARFMFGKEPTIILKAYEKEHKEACEELR
                     QYIDSILNASKFWSNTLKAFKIATITKRVLLRLEAEPNQPIRLFYHSINDFKYQVDSN
                     DITKLKSVVFVRFDSSTIKEVTAKQIWYRYTYYMKKSNVSNQESCFIKIEKFKGDNLS
                     KPIEVKESDTKLSKIPCWVIVNEQSITNITGISDIEDLKPLQDTYNKRLSDFNDSLKF
                     LMFGQTVVVDATEETVNACKIAPNALMALKTLEEGSEKGKQAQAYRVESSFSNADPVN
                     SFFKRLEDSMYEKLAIPRPEQLQNIPSAKALKYLYTELIARCSEKWNDWEPAIRSMLR
                     LIVEACSKFNCYDDWNHDWDDLMFSIVLNKNYPIPEDEEDSKRLALEEVNNNVRSHRN
                     YIKEFGDDEDYEEAFNEVLEDNEKIQSVEQDQFRKDAEIEIDNIDEELNSKSNNSDE"
     gene            36620..38239
                     /locus_tag="KRL67_000206"
     CDS             36620..38239
                     /locus_tag="KRL67_000206"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH1521118.1"
                     /translation="MKDNTYTKKVLEARKKLLLLDKKVQLEILSVYKDASKTILSDIV
                     KNKELNLSTKYLKKLNKSIEKYINELNQRLVPVTEKSIIEASNIAKELQMYYYQSIVP
                     NESIKFACDAMCIKTTTSVVEKLVASNFYKDKRSLDSRIWGYSNKNRKDIDRLIKANV
                     ARGANAKTLAKSLDNYVNPVKKTEAKTLEVGMNKSISYQAQRLARTSITHAFVETSVQ
                     NAINNPMCIGLQWNLSSQHYIRQVKWRGEDECDEYAEQNRYGLGEGVFPPEKYPIPHP
                     NCLCYPTQVIVPINEASKMMNDWATGGDNDILDSWFDGIIHQNNSIPVIKKQINKKNK
                     ITTNSKDDRIKKNLTNSIKNKSKKAPKQIQKYINKYVNTNKIIIDNGQKIPFVYYTKV
                     DLVGINPNIREFKSYNKEAALLHEFAHRIDIKEIKSYNNIKFQKAIESSSIYVMENIE
                     NLQSIYTNSNELYNNEFISDILGALSNNEFEDLLATHSEKYWSKNRNKEKEIFANLFT
                     LKYQNNKEINSFIKEHLNSLDKIFNELLGGI"
     gene            38241..38393
                     /locus_tag="KRL67_000207"
     CDS             38241..38393
                     /locus_tag="KRL67_000207"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH1521119.1"
                     /translation="MLEEMRKDKKLLELRRLYKEKYGKNAPGFNYDEYNSYAEYKEKL
                     KELIQK"
     gene            38460..39113
                     /locus_tag="KRL67_000208"
     CDS             38460..39113
                     /locus_tag="KRL67_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003494198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage scaffolding protein"
                     /protein_id="HBH1521120.1"
                     /translation="MLEYFKKLLGDEEGQKVYEKLSKDKENKLLLDNIKSPRYVEKTE
                     LENANKEIKEYKKQIGDRDKQLNDLQGKVKDNKELSDEIESLKNANKEIRENAEKEIE
                     VLKFNTAFDRAIESYNPRNPKALAALINKENINFVDGKIIGLDEQIKAYQQSDSYLFN
                     NEKNKGGDEIGGTGGLEGGTTSLLDNDSEISSIGELLANSKVKSENIEAQKKFFGEE"
     gene            39125..39481
                     /locus_tag="KRL67_000209"
     CDS             39125..39481
                     /locus_tag="KRL67_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003494196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH1521121.1"
                     /translation="MSIEKSEIYMGENKTILKFAGNLFQNVNIKVKKTDVAIVDEKRI
                     LKAGTLISKDGKLVDGTTVTNDKAFGLVYRDIDFTYSNGNESIPVFIFGFIDEKTLPT
                     AIPEEAKQAMKMIMFL"
     gene            39505..40533
                     /locus_tag="KRL67_000210"
     CDS             39505..40533
                     /locus_tag="KRL67_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011099336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major capsid protein"
                     /protein_id="HBH1521122.1"
                     /translation="MDWKDFIDSKEIAKYIKKLPLEMLIGEALFPRKKQIGMDLKYIK
                     GAKKKPVVLKQSTFDVAVKIRALKAQIEVKSKRMPFFKESVLVNEEDRQQLLLASQAQ
                     NKELLLMIISQIYDNYLALVDGGDMQMERMRMQALADGVINIVSEDGDLVFDFEVPSN
                     HKEVLTGSATWDNPDADIIGDIQRWMRIMRDEGNPLPKRMVMTSKTFGYFAKNKAIKL
                     DIDKDGRVILTDEMIKNYLKNKVGLSVAIVSGTYKLEDESEESYFPDNKITFIPDGDL
                     GKTYYGTTPEEADKVYGSKLDCSVVRTGIAITTMRLIDPVTVQTKVSQLGMPSFERAD
                     ECFFATVA"
     gene            40551..40757
                     /locus_tag="KRL67_000211"
     CDS             40551..40757
                     /locus_tag="KRL67_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003378055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH1521123.1"
                     /translation="MAKKKDNLMQVKALVYLKYDNGCYKIGDVFEIRKADQESMEEKG
                     YIEVIGEIEEKNNSDNELLEKDGE"
     gene            40760..41077
                     /locus_tag="KRL67_000212"
     CDS             40760..41077
                     /locus_tag="KRL67_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002581887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH1521124.1"
                     /translation="MSITNLDKLKLNLQEEEYPYFTDEQLVMLLESNENNVLKASWRG
                     CLLKGATDDCIKIGPIETKSSNSSYWLSLADIYKTDYLEEKSKNETTNTGYKTSMIRV
                     DGQ"
     gene            41074..41457
                     /locus_tag="KRL67_000213"
     CDS             41074..41457
                     /locus_tag="KRL67_000213"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH1521125.1"
                     /translation="MRKLRADKIIKTINRGITLNPQTITIEQEVKNIVDGAIEVTNEV
                     KELTVVIYPEKTNDTVINSETIGTAYKNKNFGMVTDKEADLRLNTESNITFKCIEGTM
                     KLNYVNPVVVEGKICGYICGLEKLD"
     gene            41468..41878
                     /locus_tag="KRL67_000214"
     CDS             41468..41878
                     /locus_tag="KRL67_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016056321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH1521126.1"
                     /translation="MSVFTKAINEIDRKKSTMPLLCMNIAFMLEGEAKNSAKWTDRTG
                     NARQGITGTSLGGGNQYIVRLGHGVDYGTVLEEGSAPHIIKPRNAKALFWNGASHPVM
                     KVQHPGTKGTHSLESTISRNMPKIGKLIEGHWSK"
     gene            41880..42704
                     /locus_tag="KRL67_000215"
     CDS             41880..42704
                     /locus_tag="KRL67_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016056322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH1521127.1"
                     /translation="MRAGIRKALIENIPKLKDCYEPTVPNKKTIKPYAVIVQGEDADN
                     EGDVIGFRRIINIWLYEKRTTFNKLDELTKEVIETLDFKTITDNTSNEVFTCIYEGAV
                     GQDVIDEEWEAIIRCLRFSVIALDDKDDITSDRWVEALSKYTKDLLEIESYKDNWKKN
                     FIAPCCLWRTTNIENKRINYHLIEITKTMKCHVVSKNKDEIIKLLEALETKLIIDKRV
                     RLREDKNMYLTLVSVVEDRESDMFTTGQLTVIFKMIGKIKREGPIMNAIYNSGNLR"
     gene            42717..42920
                     /locus_tag="KRL67_000216"
     CDS             42717..42920
                     /locus_tag="KRL67_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015994350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH1521128.1"
                     /translation="MAETNNKKTNVSKQEEKYLKSDFIKNSEALGYKKEVVAGALFNC
                     KKDELTKSEFEKLIKDFLEREVK"
     gene            42922..44343
                     /locus_tag="KRL67_000217"
     CDS             42922..44343
                     /locus_tag="KRL67_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015994351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath subtilisin-like
                     domain-containing protein"
                     /protein_id="HBH1521129.1"
                     /translation="MATGTWNEKEKKEIPGFYNRFKTQAEKSTNKGLKGRLAIPVKAN
                     WGEVGKVVTIKNDLRQLKTLFGDDMNYSAYKLGKLALLGNVKELLLYRLVDGNQKKGT
                     LTLKDTTENTAKDVIKLETKYPTSRNFNVTIKSNLVDADKKDFVFFEGTKQLFSSSVK
                     GTIDEIVLEINSNLDNEYVIATKVADSDTTLANLVNVALEGGNDGCTSITNESYLKAL
                     EEFERYSFDSFVLDGVADEALQETTKAWVAKNKELGKDILLFLGGKTEDNIKQINDKS
                     KGFNDENIVNVGSSAYYEGIKYTPSEVAVYIGALAVSKGITGSICNAKTIFEEVEPRL
                     SQSEVKECLKSGTLILDFDDGDVIIVDDVNTFKKYTDDKNEAMGYISNIMFINTINKD
                     TSLKRKEFVGKIFNDSAGQTTVICALKKYFEELMSQGIISEFNVDIDTELQATAKADE
                     FYWKWNAVKVDVMKKIYGTGYLG"
     gene            44359..44772
                     /locus_tag="KRL67_000218"
     CDS             44359..44772
                     /locus_tag="KRL67_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016056325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH1521130.1"
                     /translation="MYNDDYIEEASFLNGSDVVILIDGVEELYMEEIKADFEQDEQSI
                     KLLGCQNEISRVGTTKGSFSLNGYKTDSRFAKLGFRSFEIIYNLSNSETLGYESIRLK
                     NCRLKKLPLINSKAGEIVKIEVEGSFRGYDLLNEL"
     gene            44856..45350
                     /locus_tag="KRL67_000219"
     CDS             44856..45350
                     /locus_tag="KRL67_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016056326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH1521131.1"
                     /translation="MSEIYKRELENEVVEEDNDLDEEIEETNEDRLKMKEDEIIAKLL
                     EDSPVPERTVFLDRLGIPITLKALTEKEISKIRKDCTKIVKVQGRREEKLNDDEFTLA
                     LIEKGTVKPNFSNQKLLDAMKVTNAREFIKRKFLAGELSKISDQILELSGFYDEISDD
                     DIKN"
     gene            45527..46513
                     /locus_tag="KRL67_000220"
                     /pseudo
     CDS             45527..46513
                     /locus_tag="KRL67_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016056328.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
     gene            46790..50077
                     /locus_tag="KRL67_000221"
                     /pseudo
     CDS             46790..50077
                     /locus_tag="KRL67_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016056328.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
     gene            50159..50887
                     /locus_tag="KRL67_000222"
     CDS             50159..50887
                     /locus_tag="KRL67_000222"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH1521132.1"
                     /translation="MWQKFKSLNTFLKVIIGIIAICFLPITLAVFSIEFLMKAIEAKN
                     KSKIILGCFLALLTLSCARTVYLTRDLDDKNSTQVNQVSSNKDKEDAEKKIKEETKKQ
                     EEVKKKEEIEKNSKEDNKGKVETKKQTLTGEELKKKVDSIIPAEYKGNYYTNDVLDTD
                     GSYVLSLQVQNASFDNESSCKVFTKDLINKLKEFRIDSAEIYFVGSSGQTTYQINIDD
                     FLKVQDNVNSVDNMEFFSFKDFKN"
     gene            50957..51628
                     /locus_tag="KRL67_000223"
     CDS             50957..51628
                     /locus_tag="KRL67_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016056330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HBH1521133.1"
                     /translation="MVIDIYLKNEKEKIDFHFPVTPQDSLSIKKEKRFETVDIVNLGE
                     FDIKKEGEKIREISFKTFLPFQYDVSYCRYSELKNPTETVAMLEKWVDQAEPLRLIMT
                     GFGYNGLVTISSFSNTQTAGREEDRDIEITFRTYRELKIETLKKETKSNAKTDLKDNR
                     PNTQSSSKTYTVTSADTLWSIAKKFLGKGSRWTEIYNIPENKKVIGKNPNVIKKGQKL
                     VIPSK"
     gene            51625..52587
                     /locus_tag="KRL67_000224"
     CDS             51625..52587
                     /locus_tag="KRL67_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015994357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdQ"
                     /protein_id="HBH1521134.1"
                     /translation="MKIILNGKYDIANFNEGITLSEAIDGVAYKMDISLVEPKQLQDI
                     GIKKGDKIVLIDIAYESKKEETIFNGVIWETRRSEKSKKLTLSCRERTVYMEESEEQY
                     SFKENTATQRIEYYCKQWNIPYYNLANTGKKLAKVIHKTNILDMIKKDLKETASKGGD
                     LFRVRMDNKLKLFKLGTNANVYKLDSILEDANFTSSFNDAVTSVKVLGKSKDENTKAP
                     IIGTYKKDSDKYGTLQKIKQDEKIKNAKEAKKAAETMFNSGEETISVDCAVDINRIRA
                     GDKVSLKGKEYYVIDVTHTLDSTPKMKLNIGTLEYTRRKFYTND"
     gene            52580..52945
                     /locus_tag="KRL67_000225"
     CDS             52580..52945
                     /locus_tag="KRL67_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016056332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH1521135.1"
                     /translation="MTDARFNGVARILKEKMNKSVNDGVFGIGCELAEITANGLKVNG
                     YKDEIQDYLVLENLTLKEDYFTLSDEMLGAEYRHKHKIETPKELKPLAIGDKVLVAVM
                     GAEFVVIGRVVNAKPISSK"
     gene            52920..53378
                     /locus_tag="KRL67_000226"
     CDS             52920..53378
                     /locus_tag="KRL67_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016056333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2634 domain-containing protein"
                     /protein_id="HBH1521136.1"
                     /translation="MPNLFPQNETFETIELKNNNESELDLKGSFLFDFEKGEFVKNAD
                     GTLKKCDKMQAYKQWCQKAILTPRYKRSAYSSVYGSEIKDLIASNLSQSAKELEITRL
                     IKETLLVHPYTKEVNNFTFNWLENSRLVNYEFDVLTKDDENIVIDGNIKR"
     gene            53387..54523
                     /locus_tag="KRL67_000227"
     CDS             53387..54523
                     /locus_tag="KRL67_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015994360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HBH1521137.1"
                     /translation="MERELPIPSFLTEDEDAVHERMLSNFQDVSTLEGDFIYDSTRPT
                     AEEITRLKQLGLQNNLKIAFPQTSYGTYLEWLGECKGVFKNQPTKSVGVITFIGIQGT
                     IITKGTIVTTVATDEKQSIEFELLETKTIGESEIVDIKAECRVAGTIGNVSKGSVSVL
                     LGSISGVKSVTNKEDFKGGTDIEDEEHFRERVLVAEQEDKLSGASSDYIRWAKEVNGV
                     GYAYVVSEWAGPGTVKVLILDKNRKAATQELIDKVQEYIYPLNTEEGQNRDGKAPIGA
                     IVTIATPQTLLINVKASFVFSNGFSQETVLNILKNKIDKYLDKIDIGGTVSYNAIYSL
                     TGSMMLADEGIQDFSNLTINDGTTNIILNDQVVGIGEIINEVIA"
     gene            54520..55149
                     /locus_tag="KRL67_000228"
     CDS             54520..55149
                     /locus_tag="KRL67_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016056335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmfQ family protein"
                     /protein_id="HBH1521138.1"
                     /translation="MITSKKGREMLLTLSPIYEQSLVMNSIYEAIGSEFDNLELLNEE
                     IELQLFPQSATWGLSFWENRVGLTTNIDEDIEIRRRKVIAKLQSKYIMTPKRMSMILQ
                     SYTGANIKINENISPYTFGVELTSTQGFPRDLEDLYKRVNVIKPSHLAVSYKLVSLLK
                     SKTYFAQTAIMSEEITVYPYTSKEVKASVKAKFALANNISSETLTVYPR"
     gene            55162..55962
                     /locus_tag="KRL67_000229"
     CDS             55162..55962
                     /locus_tag="KRL67_000229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009900142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBH1521139.1"
                     /translation="MADEQFYTILTNIGKAKIANAGMLGKSVVLEKIQAGDGGGNYYN
                     PTEDQTALKNKVWEGNINAFDNDENNPNWIIATACIPGSIGGFTVREMGLIDNEGDMI
                     AVCKSPETYKPKVDNGAMKDLYLKFIIEVSNVEKVTLVVDPTAIFLTKKDEEKILSNI
                     NKLDTKLDTTKTELTNSIETAKTELNNKIGDTTQLETTDKTSLVGALNEVKTSVDSIE
                     TTAEKTSYNNATSNLTATNVQGAIDEVVRKIENFNEVNISIQNDMLPI"
     gene            55980..57212
                     /locus_tag="KRL67_000230"
                     /pseudo
     CDS             55980..57212
                     /locus_tag="KRL67_000230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901445.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            57251..57433
                     /locus_tag="KRL67_000231"
     CDS             57251..57433
                     /locus_tag="KRL67_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015981517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH1521140.1"
                     /translation="MNINNVVVRILAERILSGGLNPLKNREFELDDVTNAEYRKAVED
                     YIIEHSGVVEGAEPTK"
     gene            57469..57699
                     /locus_tag="KRL67_000232"
     CDS             57469..57699
                     /locus_tag="KRL67_000232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin XhlA family protein"
                     /protein_id="HBH1521141.1"
                     /translation="MNEEVIKEKIKRNEIRINRHSDEIDELKIANIESKAELKALCEN
                     LNSLTSMLKWLIGTMITTLVGFFIFAIQKGIF"
     gene            57719..57976
                     /locus_tag="KRL67_000233"
     CDS             57719..57976
                     /locus_tag="KRL67_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HBH1521142.1"
                     /translation="MDNLISFIPEQLLILVAALYVIGAGCKKYKQLDNKYIPVVLLVL
                     GIGFSIWMLGLNPSAVLQGVICWGISIGINQTYKQLREENE"
     gene            57976..58734
                     /locus_tag="KRL67_000234"
     CDS             57976..58734
                     /locus_tag="KRL67_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HBH1521143.1"
                     /translation="MVDIIKMLTKKKCYPNKNSPKFIVIHETDNEDKGADAKRHAQAL
                     NNGNLEASVHYYVDDKVIYQTLDHKDGAWAVGKSYGTALVAGVTNYNSINIEICVNRD
                     GNYTKARQNAIDLTKKLMKDLNISADKVIRHYDAKRKYCPRKMLDNPSLWVDFKDKIK
                     NGGGVNSMVKYTIVYDGEVDRIPAEVMGWNYKKEECRVCDIKDYVPGQTQNLYIVGGG
                     ACNKISSMTKEKFTMIKGNDRFDTLYKVLDFIDR"
BASE COUNT        23835 a         6414 c        11163 g        17712 t
ORIGIN      
        1 taaagttacc tatataggaa atcaaaataa attttataga gagtgtagta agtatctttt
       61 agataaacca tctttaaatt atattatagt atattttgat attaataact ttaagatgat
      121 taatgatact tttggatatg aatttggtga caatttatta attacgatag caaaagcttt
      181 aaaagaagaa ttaacagaag gtgaagtata tgcacgactt tcaagtgatt attttgcaat
      241 tttctgtgat tataaaaatg gaagaaataa aattataaga aaacttgata gtatacgaaa
      301 taatatcgaa agtaatttga gtatagtctt tgagatttct ctgtgtgtgg gtatttattt
      361 tgttgaagaa ggtgaagtag atattcaaaa ggctgtaaat aaagcaaata tggctaggtc
      421 tgttgctaaa ggtaaaaata taaattatgc tatttataat gaagatgtta ggaataagct
      481 tagtgaagaa tcaatgattt tagatgatat aaaaatagca ttggtaaaaa atcaatttga
      541 agtgtattat caacctaagt tttcacttgt aaatggagaa atgataggga gtgaagcact
      601 aataagatgg aatcatccag aacatggttt tataagtccc gcagtattta ttccaatagc
      661 agagaaaagt aagttgattt taaaaattgg gagatttgta tttgaaaggg tttgcactga
      721 tttatctgaa tggaaaaagc aaggtaaaaa gatagttcca gtttcagtaa atttatctag
      781 agttgagttg tatcaaccag atattgtaaa gtttatcaat aaaacaatac aaatgtataa
      841 tctaagttca gatttaattg aaatagagat aacagaaact gtggctatta atgagttaaa
      901 tattttaaag aatattttaa atgagttgag gaagtatgga ttttctattt ctatggatga
      961 ctttggtaca ggatattctt ctattagttg tttaagagat atgccaattg atatattgaa
     1021 actagataag tcttttcttg atggtattga acatgatgag agaagtcgca atatagcaaa
     1081 atcaatagtg tctctagcaa aatcactaga tttagttgta attatagaag gtgttgagag
     1141 taaagaacaa gctgagttaa tgaaacaatt tggatgtgat ttagttcaag gattttattt
     1201 tgcacgacca atgccagcta agaatttttt agatttactt taaaacaaaa tttgattttt
     1261 atataaagca ctattatgaa tgcttgattt taataaatca ctgctaaata attagtgttt
     1321 ttttatttta tcaatgtatt gtctaatata aagcaaaaaa taattgtatt ttataacaat
     1381 tatattaaaa ataaatttat tttgctaata aacatgttat taacaaaaga atagtactat
     1441 aatgtataga tgataaaaac tttaacaaaa atttagtgta taggagttgt ttaagtatga
     1501 tagatgtaag tattgatgac aaacttaagg aaagatggcc taatgctaag ctgggttgca
     1561 ttcaagcaaa agtagcagta aataaaagct cagaaaaatt aattaatgaa ataaatgagt
     1621 tttgtgatac tataaatcaa agtctaaaaa ttgaagacat tacaaaacta gataaaatta
     1681 aagatgcaag aaatgcttat aaagagcttg gaaaaaatcc aagtagatat agaacgtctt
     1741 cagaagcact ggttagaaga attattcaag ggaagggtct ttatacaata aacaatatag
     1801 tagaaataaa caatttaata tcaataaaat cattatatcc agtaggtaca tataatgtaa
     1861 gcaaattaca ttcaccagta tgttttactg taggagatga aggggaacag tataagggga
     1921 ttgggaaaga attaataaat atagaaaatc ttcctatatt gtccgattca gtgggtaaat
     1981 ttgggagtcc aactagtgat tctgaaagag caatgataac agaagatgca aatgaaatat
     2041 taatttgtat attttctttt agtcaagaat ctgatatcga agaatatttt gggtatgcta
     2101 aggagttact taaagaatat gctgatggaa aagatataga aactaaaata attaaataga
     2161 atctatataa caacgttatg ttaagctaaa attcaatata aaaataagtt aagataaaga
     2221 aactaacata aaattagttt cttttttaaa agagtagttt agtgttataa aaacagatag
     2281 ataacccacg attattaaaa gtatggtaat atattaatat agtagagctt gtatttaagg
     2341 gattttgaaa ggggtgattt ataataatca ttttatctaa aagataaaat ggttttatgg
     2401 aaataactat atatttaaaa atataaataa tgatacagta atttgggtat ataaaataag
     2461 acatacatgg ttttgtttaa aaaccaataa agtacatatg taattacaat gtaaggaagg
     2521 atttaattat gattgatgga aaagaggtaa aacaagaaag tggacaaaaa aatctaaaat
     2581 ttaagatagc tatagtagta ggtgttttgt ttgtagctta tataggaatg gcaatctttt
     2641 ttagaaatca tttttacttt ggaacgacta ttaatggagt aaaggcttca ggaaaaacag
     2701 ttgagcaagt aaatgatttg ttaacgtcta gtactaattc ctatacttta aacttagagg
     2761 aaagaaacaa taagaaagaa caaataaaag caaaagatat tgatttaaaa tttacaccag
     2821 atgataaagt tcaaaagata aaagatagtc agagcgctat tggatggatt ggtggaatat
     2881 ttaagtctaa agagtataat aatatggtga atctttcata taataaagat ttattagaaa
     2941 aaagatttaa cgctttatct tgctttgata gtaaaaatgt tgtagaacct aagagtgctt
     3001 atcctaagta tgatagcact aaaaatgctt atgttatagt gccagaggta cttggaaaca
     3061 aagtgaaaaa agatgatttt tataagttag tacaaaactc aattaattct gggaatactt
     3121 ctatttctct ggatgaaagt aaagtgtatg aagcaccaaa caacacaaag gattcagaaa
     3181 agttgaaaca agctattaag acactagata agtatgttgg cgtagtcgtt acatatgatt
     3241 ttagtgaccg taaggaaact ttagatggtt ctgaaatcaa taaatggtta aaagtagact
     3301 cagaaaagaa ttatgacata acttttgata atgatgctat ggtagaatat acaagaggtt
     3361 taagtaaaaa atacagtact tttggagact caagaccatt taaaactgcc acaggtaaag
     3421 atattacaat ttcgggtgga atttatggtt ggttgataga caaaaagaaa gaagctgaag
     3481 cattagttga tgttgtaaaa gctggtaaaa atgttacaag agaacctatt tacatacaaa
     3541 aagctgtatc tcgtaataga gatgatattg gaaatacata tgtagagatt agtctttctg
     3601 gccaacatat gtggttcttt aaaaatggag aaatgcttgt atcaacagac gttgtaacag
     3661 gcgatttagc tagaggattt gctacaccat caggtatata tcctcttaat tataaggcta
     3721 gaaacgtttc attaactggt caaggttaca gttcacctgt aagttattgg cttccattta
     3781 atgggaacat aggcattcat gatgctactt ggagaaattc ttttggtgga ggtatatata
     3841 agtcttcagg ttcacatgga tgtgtaaata cacctttttc taaggcaaag aaaatatatg
     3901 aaaatataga accaggtaca ccaattattt tatactagta aatgtgttgc taacagttaa
     3961 gcgaagtcat cttagtttat ttaggatggc ttccttttta tataaagctt attgaagttt
     4021 acataaagac tatttaaaag gagaaataca taatgaatga aaagttaact aaaattttag
     4081 aagaagctaa gaatgatgat gaatttagaa aggaacttat aaaaacaaga gaacaacgtg
     4141 atgttatgga ttcattttgt aaggttgtta catctagagg ttatgatata acagtagggg
     4201 aacttttttc cataggagaa gaatatacat ccaatctact gaaaagtgta aatggaggag
     4261 cagcatatcc tattgaagat tggagtgatt ggtacgagga tttttttata atgttgtcga
     4321 atatatagag taataatgat attatctact taagattaat acattgcctt aaaataaaac
     4381 tattagaact aagtgtaact tttatcaatc tttaaaaata ttgatttatt tctaactaga
     4441 tataaaaaat ttagattaaa aataaggtga caaaaatcga tatagcttga tgatttataa
     4501 aagttagata aaatagtgcg agatatttct aaaaattcaa aaaaaacaat aaacttcaaa
     4561 ggttagaggt tgaaaaagaa tataaaaaac ttttatatgt aataaattaa attgaaaata
     4621 taaaaaaaat aaaataaaaa tttgaaatgt cgacataacg ttaattttaa aacaaaataa
     4681 cgctattgac aaactatatc gctttaaatt ataattattg aaaataatgg tatttaaaaa
     4741 ccatactcat gtcatagtct aaaagtaagt caaaaaagga tgtaaatcta ggcaagtact
     4801 attctaaaat atgttttatt aagaaataaa cgttgaaaaa tattaattat tatataaaaa
     4861 tgctgtttat actttaacaa tgaggtagta atctaattag aataaaaaaa tctaattggt
     4921 tatatacata gtaaaaaggt aagagaggtg atttggatgg cttttgaaat aataaaaagc
     4981 attgttgagg cagagcagac agcagacagt atcaaagtaa aagctgttac tgatgcagag
     5041 tctatcagag ctgatgctgt aaacaaatgt gaaagcatat ttgctgatgt aaaaaaacaa
     5101 gcaaagctta tggaagaaac tcttattgag aaggcagtca ccgacagtag agcagaggtt
     5161 gataaaatct tagctaatgc taaaagtgaa tgtctgaaaa ttgaaaaaac tgctgaagaa
     5221 agaaaaagta aggctattga agcggttatt ggaaaggtag tgagataata tggcaatagt
     5281 taatatgaac aaaatatcta ttgttggctt agaagctcaa aaaagccaga ttttaaagct
     5341 acttatgaac cgtgggtttg ttcagattga tgatagtgcc tttcttactg aggaggagga
     5401 attaaaaaat tatcttgtaa aagatagtga agaatctgaa gttataatgc tagagcagaa
     5461 aatgtctcat gtaaaccaag caattaatat tgtttcttca ctagtgaaac aaaaaaaatc
     5521 cctacttgca ccaaaaagag agtttaacaa aattgatagt gaaaaagaag ggcaagttta
     5581 tgctgaagct ctggagttaa acaatttgca caaggagata ggtacaataa ggtctaacat
     5641 aaacttactt aacaacaata aaagtatgtt agaaccatgg gtagactttg atattcctct
     5701 tgaaaatatg gaaactaagt atacaaaaac tatacttgga actgttccca taagcgcaaa
     5761 cattgatgct ttaagagtaa aattagaaga tgaaatacca gaaagtgtta ttgggattgt
     5821 aaattctgac aagttaatga actatgttta cttaattact ttaaaagaaa cttttgatga
     5881 tgttttagag gttttaaaag aatttaattt ttcagtagtt tcattaccaa atgaagaagg
     5941 tacaccgtta caagctatca agaaatacaa tgaaatgata attagacagg aaaacgtttc
     6001 taaggacaaa acagaggaaa ttaaaagaca tgcagatagt atacacatgc ttgaaaacct
     6061 atatgatttt tacactatag aaagagacca aaaaaagatt atagagagac ttgtcaaaac
     6121 aaaaacaaca ttctgtctta atggttggtt accaagcaaa agggcagatg aattaattaa
     6181 agaaattaca gaaaagtttg attgttgtgt tcagactgaa gaaggaagta aagaagaagg
     6241 cttcccaatt ctgcttgaaa acaataaatt agtaacacct tttgaaagtg taactaacat
     6301 gtacagctat ccaagtacaa aagatattga cccaaataca attttaactt tcttttttgt
     6361 tgtattcttt ggaatgatgc taagtgacgc tgcatatggg ttaataattg cagtagtctg
     6421 tggatttgtt gtatataaat taaagataca aaaaggtgaa ggaaatttaa ttaaacttat
     6481 tggtatctgt ggagtttcaa ctactgtatg gggtcttata ttcgggggta ttttggggga
     6541 tttaattcct ataaaagcat taattaatcc tttagaggat gttatgacac ttatgggaat
     6601 gtcgttactg ttcggtatta tccatatata tgtaggtttg ggaataaaag catatacatt
     6661 aataagaagt ggaaaggtag tagatgcaat ctttgacata ggtttttggt atttgtgtat
     6721 aacaggtgta tgtctactta taataccttt tgttgcaggg gacataggtg tattttcaga
     6781 agtaggaaaa tatttagcaa ttataggtgc tataggtctt gtacttactc aaggaagaag
     6841 ctttaaaggt attcctgtta agctttttaa aggattttca agtctttatg gcataacaag
     6901 ttattttgct gatatattat cttacacaag aattatggca ctttgtttaa caacaggagt
     6961 tatagcacaa gttatcaacc ttttaggggc tatagctggt ccaatacttg cagttgtaat
     7021 cggtgttgta ggacacacaa taaaccttct tataaatgca ctaggtgcat atgttcatac
     7081 aagtaggtta cagtatgtag aatttttcaa taagttttat gaaggcggag gagttccttt
     7141 tgttccgttt aagtataaaa caaaatatac aagcattaat aaaaaggaga tgtaaatatg
     7201 gaatttttaa acgcgttagg aaatggtcaa tttttggcaa tatcaggagc tgctatagca
     7261 gcattatttg caggtatggg ttctgctaaa ggtacttcaa tagcaggtca agccgcagca
     7321 ggtgttgtaa cagaagaccc tagtaaattc ggtcaacttc tagttttaca acttcttcct
     7381 ggtacacaag gtctgtatgg atttatcata gctttcctta ttttatcaaa agcagggatt
     7441 attggtggag atgcaatacc aacatcagct caaggtttac aacttcttat ggcaggactt
     7501 ccaattggtt tagttggttt agtatcaggt atagcacaag gtaaagcagc agcagcagga
     7561 gttggaatag ttgcaaaaag acctgaagaa cttggtaaag ctataacatt tgcagcaata
     7621 gttgaaacat atgctctttt aggactactt gtttctttct tagcttacaa tggaattgca
     7681 attggttaat tataaattat aattcataag taaaaaaaat gaaaaggagg aagcactcct
     7741 atgggtaatg aacaaaaaat gattgacaga attatagctg atgccaaaca agaagcacaa
     7801 gaaatattgg ataaagctaa aagtgaagca gatttaaaag taaattcagc taatgaaaaa
     7861 gctgaaaagg aaatggcttc ctatacaaag cttgcagagg cagaagcaga aaaagcggct
     7921 tctaaggaaa tatcaggagc ttatatggag gctaaaaagc aaatattatc aaaaaaacaa
     7981 gagattttag aagaggtaat tttagaagct aaaaacaaac ttttaaatct taaagataat
     8041 gaatatgaag agattatact aaacatgatt gaaaaatcaa attgtacaga tgattcagag
     8101 atagtattat ctaaaaaaga taaaaaaaca ttaaaagatg tgctatcaaa aaaaggcatt
     8161 aaagtgtctg atgaaactag agatattact ggaggtttca tagttaaaaa aggtgacatt
     8221 gagtacaatt attcatttga agccataatt gcagtagagc atgaatatat tgaacaaatt
     8281 gcagcagaaa ttttgtttaa ttaaggaggg gttaaaaaat ggcagaagaa aaaacttatc
     8341 cctatgccgt tgcacgaata agagtgcttg aaaaacagct tcttaacaga caaatgttta
     8401 ttcaaatggc agaagccaaa agccctgaag actctttaag aattatagct gaggcaggtt
     8461 atgctgatac atcaaataat aatatacata attttgagaa tattttgact caagaattat
     8521 caaagacata tgaaattctt aaaagtctag ctcctgaaga aaaatttgtt gatgtatttc
     8581 tttataaaaa tgactatcac aatttaaaag tgctgattaa agaagaaatt tctggtgtag
     8641 atggtgagaa atatttaatt gatggaggta caattcctct tattaagctt agagaatctt
     8701 tagcaaatcg tagttttagt gatttaccta agataatgtc atcagctata ttagaggcat
     8761 ttgatgttta tagtaagaca caaaatggcc aaatggttga tattgttcta gataaagcaa
     8821 cctttaccag tatgaaagaa actgctaaag aaagccaaaa caaatttgta atagattatg
     8881 ttatgaaagt ttgtgattta acaaatctaa agagttttat aagagtcaaa aatatgaaga
     8941 aagattttga tatgtttatg aatgtgtttg tttcaggtgg ctcacttgat aaggaaaaat
     9001 tcttagaagc tttttcctca gatactccag catcatgttt taaatctact tcttactctg
     9061 atgtctgtaa aaatggcatg gatagtggtt ttactgtctt tgaaaaactt tgtgatgact
     9121 atcttatgga atatgttaga ggagcaaaat ttaagcctct tacattagaa ccactaattg
     9181 catatttata tgctaaagaa tcagagataa aaacaattag aattattttg actagtaagc
     9241 ttaataatat agacgcagat actataaaag aaaggctaag ggatgcttat gtataaggta
     9301 ggagtagttg gagataaaga tagcatcatg ggatttttag ctcttggaat agatatcttc
     9361 ccagcctatg atagtgacga aattaaaaaa agtatacata agttggttga agatgaatat
     9421 gcgataattt atattacaga acaggcatca ttgcttgcaa aagaatcaat agcaaaatac
     9481 aaggattacc aacttcctgc cattatagtt attccaggta ttggaggaag tatgggatta
     9541 ggtatgaatg aagtaagaga atctagtaaa cgtgctatag gtgcagatat attatttaaa
     9601 gaatagatga aagttgaata aaagacaatc tcaaatttta ttggtgctaa atatatgtac
     9661 taataaaata taggagggaa tatttaaatt ttgataaagt atatgattgc aggtgagaga
     9721 atatgaaaac aggcacaatt gtaaaggtgt caggaccgct tgttgtagcg gagggcatga
     9781 gagatgcgaa tatgtttgac gtcgtaagag tttcagataa acatcttata ggcgagatta
     9841 tagaaatgca tggagataaa gcttctatac aggtttatga ggaaacttcc ggtctaggac
     9901 caggggaaga agtagtttct cttggaatgc ctatgagtgt tgaacttgga ccaggtctta
     9961 tttcaactat ttatgatggt attcaacgtc cacttgaaaa aatgtatgat atttcaggta
    10021 ctaatataac taaaggtgtt gaagtatctt cacttgatag aacaactaag tgggaattta
    10081 aacctaaaaa atctgttggt gataaggttg tagcaggaga tataattggt ggcgttcaag
    10141 aaacagcagt agtagaatgt aaaattatgg ttccttatgg agttaagggt actataaaag
    10201 aaatatattc tggtgaattt actgttgaag atacagtttg tgttattaca gatgaaaaag
    10261 gtaatgatat acctgtaact atgatgcaaa aatggccagt tagaaaagaa agaccatata
    10321 gagaaaagaa aacaccagat tcacttcttg ttacaggtca aagagttata gatacatttt
    10381 tcccaatcac aaaaggtggg gtagcagcta taccgggacc ttttggaagt ggaaaaactg
    10441 ttacacagca tcagcttgct aaatgggcag atgcagatat agttgtatat ataggctgtg
    10501 gcgagcgtgg aaatgaaatg acagatgttc ttcttgaatt tcctgaatta aaagacccaa
    10561 gaacaggcga gtcacttatg caaagaactg tgcttatagc aaatacatca gatatgccag
    10621 ttgctgcacg tgaagcttct atatacactg gtattacaat agctgaatac tttagagata
    10681 tgggatatag tgttgcactt atggcagact ctacatcaag atgggctgag gctcttagag
    10741 agatgagtgg tcgtttagag gagatgcctg gtgaagaagg ttatcctgca tacttaggtt
    10801 cgcgtcttgc tcaattctat gagagagcag gaaaggtaaa ttgtctaggt atggatgaaa
    10861 gagaaggaac acttacagca attggtgcag tttctcctcc tggtggtgat acatcagaac
    10921 ctgtcagcca agcaacgctt cgtatagtaa aagtattctg gggtcttgat gcatctttag
    10981 catataaacg tcatttccca gcaattaact ggttgactag ttattcactc tatcaggaaa
    11041 aagttgatgt atgggcagat aaaaatgtaa atgataattt ctcagctcaa agagctcaag
    11101 ctatgaaact tttacaagtt gaatctgaac ttcaagaaat agttcaattg gttggtgttg
    11161 attcactttc agatggagat agattgatac tagaggttac tcgttctatt agagaagact
    11221 tccttcatca aaactctttc catgaagttg atacttatac atcacttcat aaacaatata
    11281 gaatgatggg acttatatta aaattctata agtcagcaca agatgctatc aagcaaaatg
    11341 ttactataaa cgatattatc aagctttcag ttcttgaaaa gattggtcgt gctaagtatg
    11401 tagaagaagg cgatatagat gcagcttacg attcaattga agatgaaata gacaacgagc
    11461 ttgcagacct tattaaaaag aggggggctg aagaactatg ataaaggaat ataagtcaat
    11521 acaagaggta gccggtcctc ttatgttaat aaacggcgta gaaggtgtaa aatttgatga
    11581 acttggagaa attgaacttt caaatggaga aattcgccgt tgcaaagttc tagaggtaaa
    11641 tggagatact gcacttgttc agcttttcga aagctcaaca ggtataaacc ttgctgaaag
    11701 taaagttcgt tttttaggaa agagtcttga ctttggagtt tcaccagata tattaggtcg
    11761 tgtttttagt ggaatgggta gacctattga tggaggtcca gaaattatac cagataaaag
    11821 acttgatata aatggagctc caataaatcc agcagcacgt gattatccgg ccgagtttat
    11881 acaaactgga gtctctgcta tagatggact taatacattg gttagagggc aaaaacttcc
    11941 tatattctca gggtctggtt tgccacatgc caatcttgca gttcagattg caagacaggc
    12001 aaaagtacgt ggaactgatt ctaagttcgc cgttgtattt gctgcagttg gtataacttt
    12061 tgaagatgct gaatttttta taagcgattt taaagcaact ggtgcaatcg accgttctgt
    12121 agtgtttgta aaccttgcaa atgacccagc tgtagagcgt gtatctacac ctagaatggc
    12181 acttacagca gcagaatacc ttgctttcga gcaagatatg catgtacttg ttataataac
    12241 ggatattaca aactatgcag aggcattgcg tgaagtttca gcagctaaaa aagaagttcc
    12301 aggacgtcgt ggatatccag gatatcttta tactgacctt gctactatgt atgaaagagc
    12361 aggaaaaatg aaagggcatg aagggtctat aactatgatt ccaatactta caatgcctga
    12421 ggatgataag acacatccaa ttcctgacct tacaggatac attacagaag ggcaaattat
    12481 acttagccgt gaactttata aaaaagatat acagcctcct atagatgttt taccatcact
    12541 ttctcgtcta aaagataagg ggattggtaa aggtaagact cgtgaagacc atgcagatac
    12601 aatgaaccag ctttttgcag cttattcaag aggtaaggat gcaaaagaac ttatggctat
    12661 acttggtgaa tctgcacttt ctgagattga tttaatgtat gctaagtttg cagatgaatt
    12721 tgagaaagaa tatgtttcac aaggatttag aactgataga actattgagc agacattaga
    12781 aatcggatgg aagcttctta gtatgttacc aaaaagtgaa cttaaacgta ttcgtgatga
    12841 atattttgaa aaatatatgc ctaaggagtg atatctgtgg ctagattaaa tataaatcca
    12901 acacgtatgg aaatgactag acttaagaaa ctccttaaaa ctgctacaag aggtcataag
    12961 cttcttaaag ataaacttga tgaacttatg aaacaatttc ttgagattgt aagagaaaat
    13021 aagcgattac gtgaagaagc agaaaatgct cttgatactg cttataaaaa ttttataatc
    13081 gcaagagcag ttatgagtca ggagtatctg ggttcagcac ttatgatgcc aaagcaatca
    13141 gtttcagtag atgtttctac gagaaatata atgagtgttg atgttcctgt gtttgacttt
    13201 aaaactgaaa acaatcaaag tgatatttat ccttatggtc ttgcttttac atcaggtgaa
    13261 ttagattctg ctatggaagc tttctcagat gctatgcaac cacttttaag acttgcagaa
    13321 agtgaaaaaa gtgcacagct tcttgcacaa gagatagaaa agacacgtag acgtgttaat
    13381 gctcttgaaa atgtaatgat accaaattac attgagacta ttaagtatat agctatgaag
    13441 cttgaagaaa atgagagagc aagtactact agacttatga aagttaagga tatggtgctt
    13501 aaaaaagcac ttgaagaaaa gaaaaagaat gatttggttg tataattaaa tacttataaa
    13561 aataagtcta acattctact ttatagaggt tagacttatt ttttatgttt aaattaaaat
    13621 gtagatatta taaaaattaa tatattgttt aaaaacagac tgtatttaat taaaaataat
    13681 atatttaaca aaaatttcta aaaattaacg aactgttaat ttacacttaa ctttatatta
    13741 acaaattagg tgtattatat aaacataagc aaaaacaata aaaaacaaat aaaaaaagga
    13801 aaaaacacaa agggggaaga tatgaaagaa tttatattta cttgtctgtg cattgtagtg
    13861 attacattta tgacaagttg catatcaaca acaccacaaa ctactcaagg aaaaggaaat
    13921 aataaactta caatatatgc agcactagag gaagagcaga tagcttatta tatggaaggt
    13981 tttagagaag agtatcccaa tatagatgta gagatagtta tggattctca tggtgtaata
    14041 gcttcaaaac tattagcaga aaaagataat ccacaagcag atattatatg gggattatca
    14101 gcaatgaaca tggtagagct aaaaaaagat ggtgcacttg aagcatataa gcctaaaaat
    14161 tatgataaag ttgataaaaa atttaaagat actgaagata atgtatcttg ggtaggaatg
    14221 agtgtcccag agacagcaat agtagttaat tataaggaac ttaagaaact aggtatagat
    14281 gttccaaaaa gttatgaaga tttaataaaa ccagaatata aaggacttat aacaatgcca
    14341 aatccggcat catctggaac agggtatctt acagtatcag ggcttattca aatgatgggt
    14401 gaaaaagatg cttacaatta catggataaa ttacataaaa atatagctac atatacacac
    14461 tcaggttcaa aacctgcgaa gttagctggc tctggagagt atccaatagg tattacactt
    14521 ggatatagag ctacacaaca attatctagt ggagaaccta tagaggttgt atttccaaag
    14581 gaaggttcag gttgggactt agaagcaaat gccttagtta aaaaagataa tataaaaaaa
    14641 gaagcaaagt tatttttaga ttgggcaata agtgattcag ctatggatag atatgcacat
    14701 gaagtagcag ttacttccat aaagacagat aatccagttc caaaaggtta ttctaaacaa
    14761 ccatttgaac agcttataaa agaagttgat ttaaactctg ctgccaaaaa cagaagtaca
    14821 gtacttaaaa cttgggaatc aaaatacggt tcaaaatctg aaagtaagta aagtttaaat
    14881 taataattta gagaaaagaa gtttagaaga aaaagtagga tataaaattt aaaatataat
    14941 tatataagga gagataagat atgagttact taaaaataaa taatgtgttt aaaagttatg
    15001 accaaaaaag ggttttaaat aatattagtt tggatattga agaaggggaa tttctatgtt
    15061 tactcggacc tagtggatac tatatgttag tccaaataat tatataaaat taatagtgat
    15121 gtttttgtta ttatctattt ctatataatc tattatagat aaccaaaaac gtctgcgttc
    15181 aaccctgctt aaatcactat acatctcatt aatatcaata tctaagaatt tttttatatg
    15241 tgatgtatca ataatttgac tctcctgttc ttcagttata ttatccagtt gagattgtaa
    15301 tttttcataa tcttttctat aagtctcttt atcaatcaaa tcctctaaat ataaatcttt
    15361 gattttatct aattttttca tgattttctt cttttcatct tttttgttag atttcttatt
    15421 tcgtctgatt ttttcatttt gtgcttctaa tttcaccata tgtttatgta attgtggttt
    15481 taagttagtc aaaaggtagt tttctatatg tttttcatta tgagttacat tgtgtacaca
    15541 gtttttattt agatagaatg tagaacatct ataagataaa taaagttttt ctccaacatt
    15601 actagaatta ccctttaatg tataaccaca ctccttacat tttaaaagac cagaaaaaat
    15661 atgataatgt atgtttttat taggagtagt ttttttattt ttttctaata ttctttggat
    15721 ttgtttaaat tgtttttttg atataatagg ttcacaataa tcttcaacat aaaatcctcg
    15781 tttattatag attcctatat aaagttcatt tcttagtata ttctttattt tattataact
    15841 gtataattcg ccatgaagat tattaattgt ttctattaaa gtcctaacag aacctgattt
    15901 ctcgtattcg tcaaatatag ctttaactat atattttttt tctttatcaa taactagatg
    15961 cttattttct attttgtaac ctatgggaat tgtaccactt ataacttctt tacgtctcaa
    16021 tttatcctga aaaacaaatt ttattctttc agatgtttga gctgcttcat tttctgctac
    16081 agatagcata atgtttatat gtaatctacc agcagcagtg ctagaatcgt agttttctaa
    16141 aatggttttc caatcacact tatgtttttc caaggtttcc attattttat aatagttttt
    16201 aacgccacga cttagtctat caattttagt cattaaaact aggtctactt tatctttttc
    16261 cacatctctt aataatcgtt gtaagttagg tctttttaaa ttagttgcag agcaaccttc
    16321 atcaatgtat ttaccaacta tgttaaaatc attctcttta gagtactgtt ctaaagcctc
    16381 tatttgtgtc cttatgctat ctccatttaa agcttgttct tctgtactaa cacgtatata
    16441 aagtgctact cttaacattt tacattcccc cttattgtat atttaagagg agctgaacgg
    16501 ctcttaaata tactatgaat tgttacaaaa aatttgcatt cataatacta aatcttggct
    16561 caaaaaatat cacataatta tctagtgtag ctgaaaaacc atatttactt ttataacatt
    16621 ctaaagcttc atttagaaac ttttcagtta catcaaggta ttctgccatt tcggttatag
    16681 tcttacagcc agcattaaaa ctatctatta tacctttaag accaattaac ttgttgtaag
    16741 ccatcaatct agctttatat tcttgtttgc tattttcaac tttatttagg tctaatatat
    16801 ttccatatga agtataatga tgtcctagtt cttctgctaa cacacaagct ttttcactta
    16861 tattattaag tgtattttta tttaaagcta ttctattatt tttatataat ccatgagaat
    16921 ttgaaattaa aggaacttct ctaacaataa tattattatc atctgcttca gagagtaact
    16981 tttcataact attcataagt tcacctacct attttaccaa ttatccatat catctaaatc
    17041 ttgatatatt ttttcaattt ctccttcttc atgtaaatgt tcattgtgtg ccgctattgt
    17101 gtctatgtga tttttattta catatttacc tatttgagta agttcatcta ctctttttat
    17161 tgcttcattt tgtccaatat cgtttaaggc attaaagctt gataaaagct tattttcttt
    17221 aattttaatt tgtttgcagt cttcgttcca ccccattata aaagcaggag attcatctaa
    17281 tgcatgtgct aaaatttcta gcttatcaag aggaatattt tttatagctc ccgtttcata
    17341 tctctgtaaa gtggacttac ttagtccagt cttgttagct aaatcttgat aagacatatt
    17401 aagttctaat cgtttatttt ttattctatt tgtaatctcc aacattttta aatcattttc
    17461 agtattcaaa tttatcacct ctataaaata tattataaag tattttttca taaatgcaac
    17521 acatatattg gaaaatataa aaaaatgttg catttttgac ttgacaatat aaaatggatg
    17581 tattaatatt gaattatccc aaataagcaa cgaggtgatg gaatgaatat aagtaaatta
    17641 aaaggaaaaa tggtcgaaaa gaaatacact caaaagaaat tagcagaaga gttgggtatg
    17701 actgttcaag ctttaaatgc aaaattaaat aatcgttcac aattcaagat tgaagaagca
    17761 gttaagatat caaatatttt gcaaatcgaa gagcctggtg agattttttt tgacaattat
    17821 atcccaaaaa tgcaacgaaa ttaaataaga aaacctaaca gataaaaatt aaaactaggg
    17881 aggtagaaat tatgagtaat aatttacaag taatagaaag aaacaacaaa agagttctaa
    17941 ctacacagca actagcagat gtatatgaaa ctgactcaaa gaatataagt aacaatttca
    18001 acaataacaa agataggttt attgaaggta aacattattt tttattacaa ggtgatgatt
    18061 taaagaattt taaaggtatt catacagaat atgaaaacct aaaatttgct tcaaaaatgt
    18121 atctttggac tgaaagagga gcaaataggc actgcaaaat attagatact gataaagctt
    18181 gggagcaatt tgacaactta gaggaaacat atttcaaggt taaacaacag aaaccaacct
    18241 gtatagaaga tgtattaata gaaagtttaa aagaaatgaa agatttaaga cttcaagtta
    18301 atcaagctaa taatattgca ttagaagcaa agacagaggt taaaacaata aaagaggttg
    18361 tttcattgaa tgctacagac tggagaaaag atacacaaca actaattgca aagatagcaa
    18421 agaaacaagg tggctttgaa catataaata tgcttagaag agagagttat gagttgctaa
    18481 acaataggtt tggagttgac ttaaacagaa gattaatcaa taaaagaaga aagatggcag
    18541 aagaaggtgt atctgaatct aaaagagaga aagttaacaa tttagatgta atacaagatg
    18601 ataagaaact catagagggg tatgtggcta ttgtaaaaga tatggcttta aaatacggaa
    18661 tatcaagtga tttaagtaag aattaggtta aataaaatta gcactttgaa aactaaatac
    18721 ggaatatttt gaaaatagag gggtgattta aatgggtaaa aaaagtgaat acacagtaac
    18781 aacagttggt gaaccaaatt tagatgcatt aggcgaaact ttagctaaaa ttcttacaga
    18841 gaaatttggg gtactaataa ctcatacata cattgagaac tattatgttg ataaagagag
    18901 agggggtgat gtagatggag ctattgaaag tgaactttaa tctagagaaa ttaaaaatag
    18961 gaaatgtagt gagtgtaagt tctaagaggt ttgggttcaa tattaattgt attgtagtag
    19021 tagcaactga ggaggaacta aatttagctt attatagcaa agataaggga tgtattgagt
    19081 atcaagcatt gataccagag gatattagat atggtgatta tattcttgaa agattaggtt
    19141 aggaggaaat aaaatggcag ctttaataat agtaggttta tttgcaatat gtttagtagg
    19201 attagttcaa aatagagatt aattaaaaag ggggtttaat tatggaaagt ttaaaaatag
    19261 taaaacaata tgttgaagga cagttaaatt tatctagttt agaaatagac aaaaataagg
    19321 agacttatga gattctcaaa aataaatcta gcagagatat gttagatgac attaatttaa
    19381 atgatgcatt aagagaagta acagtaaacg aaagattaaa aatatttgct gagtcattac
    19441 tagagttgtt agatacacaa atcaaaataa aagagagtga agaaagtgag gactataaaa
    19501 gactttgtat gtacttagac gaatttggaa gagatagacc tatagatgtc cagatataaa
    19561 aaaagagcca ttaggagtgg ctcgattcag aaaatattta aaaataaaaa tatgactata
    19621 ttatagcata aacggaggga aattatgagt actttatatg aattaactac agatttatta
    19681 gaaatagaag aaggtttaac agaaacaaca ggaaatgaag ctgaaaaact agaggaaata
    19741 aaagaaataa taaaacaaga gatacaaaat aaaaacacta gaatagtgtc agtaatatta
    19801 aacattgaca gtgatataaa ctctatagat tcagagatta agaggttaca agagttaaag
    19861 aaggttaaga agaatactct tgatagatta aaaagcaata taaaagactg tatggaatta
    19921 cttggtacta aaaaagtaga aacattttta ggaaatataa gtataagaaa gtcagcaggt
    19981 agcttagtca tagaagatga agaaaagata cctgctatat ataaaacagt agagcaagtt
    20041 ataaaagtag ataagaatgc tataaaagac tttattaaaa aaggtcatga aattgaaggt
    20101 tgcaggattg aatatggaac tacactaaca attccaaaag ctaaaaaaga gtaggtgtca
    20161 aatatggaat ctaataatat ttacattaaa ttaatggatg taagagttaa atttagtaag
    20221 ttgaatctaa agaaaagtgg agaaaataag ttcgctaact tcaagtattt tgagttagca
    20281 gactttctac cacaagcaac tggattactt gaagaagcta agctatgccc tatagtgacc
    20341 tttacaaatg aatatgcaac tctaacatta attaatggag aaaacccatc agaacagatt
    20401 gtatttactt ctcccatgag agatttacaa cttaaaggtt ctaatgaatt acaggcacta
    20461 ggaggtatag aaacctatca aactagatat ttatatattc agttacttaa tataactgaa
    20521 agtgacactt ttgacgcaac tagtggcaaa aatgaagcta aaagtaattc taacaataga
    20581 attttaacag ataaacaatt aagtaggtta tatgcaatag caagtaatgc aaatgttgat
    20641 aaagaaagtt tgaaagaaaa agtatttaaa agatttggaa aagagataaa agatttaaca
    20701 aaacaagagt atgacactat ttgtaatgct tatgaaaata agcaataagg gcaggtgata
    20761 ttgtggggat tataagagta agcaaagaca aagataatcc atatgtggtt ttaaataaaa
    20821 cttgtttgga agatgtaaaa ttaagctggc aagcaaaagg tttacattca tatctgatta
    20881 gtaagcccga ccactggaaa atctatgtta atgatttata taaaagaagt aaaaatggga
    20941 gggatgctac agcaaatatt ttaagggagc tcatagaaaa tggatatata acaagaacac
    21001 cttgtcgaga ttctaatact aataagatgc ttggaggata tgattatcaa gtatatgaga
    21061 taccacttga aaatcctcag aagctaaaat cccgaaaaac tgatttcccg gaaaccggat
    21121 ttcccggaaa ccgggtttct cggaaaccgg aaaacacgga agtagtaagt aatgacttta
    21181 aagtaaataa tgatattact actattgtta ttaatgaaca acctcaacaa gatgaaacca
    21241 cctatataaa aaaatacttt gaaaaatata taggtgtgat tactcctaat aactttatag
    21301 agttaatgag ttacttagat gatggaatgg aagctgatgt aattataaga gctattgatg
    21361 aagcaatagc aaatggagtt aagaattata agtatgtaaa gacaatctta aataattgga
    21421 tagaagcagg tgtaaaaact gttttagaac ttacagaata tcagaatgaa tttgaaagaa
    21481 agaaaaagag taagcaggaa aagaagcagt ctaatagtaa aactgtgaat actcataatg
    21541 tgaataaaaa taagtttgct aacttcaatc agactttcac tcaatatgaa gaaaaagagc
    21601 tagatgagat tattaaaaag agtcagaagg aaaaatttaa ataaaattaa acttctagga
    21661 agtaaatatc aatatattac ttcctggaaa ggggaggtat aaatggcgag aatatatgca
    21721 caaagaagtg gttctttaaa tgaacaagat agattggagt tattaagatt acttgggaaa
    21781 gctggatata cagtaaaggt tgctagagag aagcaaaata gcaagacaac ttatacttac
    21841 tttgttgagt atacagaaga acaggaagaa aaatagaagg gggctagtta aatgaataca
    21901 ataactttag ttggaagatt agttgcagat gcagaattga agtaccttcc aaattcaggt
    21961 actccaaaaa taaccttttc aatggcagta ggtagaaggt ttaaagataa aaatggaaat
    22021 aaaataactg attttattca atgtgagcaa ttaggaaaac atgtagagaa tttagtgcaa
    22081 tatcttgtta aaggtaagcc tatatatgct gttggagagt taaatatata taattacaaa
    22141 gatgaaaatg gttgctggaa atctattact aaggttaatg taaatgcttt agaactactt
    22201 tctagtaaaa atgataataa tgctaaacaa gaatatgtac caccaggact ggacccacaa
    22261 ggttttcaag caatagatga tgacgatata cctttttaat taagttaaat agtctaggga
    22321 gtaactatac aatattactt cctaggagtt aaaaatggag ggataaaatg agatttgaga
    22381 taggtaaaac ttataagttt gatagagaga aatttatagc atgcaatggt atagaacact
    22441 ataaaagatg tgaagaactt tggattgaag atgttgaaga tgttgaattt actgttaaga
    22501 aaacttttga tgatggctat gttcattatc caaatgaatt ctattttgct ttcggtatag
    22561 ttccagaatg gtgtgttgaa gttaatgaag tagggggtat tagggatgat aataattaga
    22621 agtcaagata gattagattt gatgagagtt aacagagttg aaataagtaa taatcgagta
    22681 tatgcaatgc ttgaagatga tattagaaaa ataggtgaat atgaaagtaa tgaaagagct
    22741 atgcaagtgt taaatgaaat acagaagttt attgaaaatg gagtaaaaaa ggattatata
    22801 gattcttgca gagttagaca taatcaagag aaagtgtttg aaatgccagt tgaataagga
    22861 gggggcgaag atatggctaa aatttggatg gacgcaggag aattgttaga aaaaactatt
    22921 gatatagaag atatgtttgg atgtaatcta agtaaaatga gaaataaaag taaacaaaaa
    22981 ggtttagttg gtagaacaaa ttctaaaggt caaggaagaa aaagtaagaa agtagagtgt
    23041 actaacatta ttactggaga gagtaaaata tttgtcagtg ctgtcgaagc aagtaaatat
    23101 ttatatttta cagaacttca tattactcgt ctagcaagaa caggaaagac tactaaaaat
    23161 ggttggaaag ttagatatat tcaagaggtg tcagatggta ttagcaaatg tggaacaagt
    23221 aattaagtta gctgaaaaga tattaaataa gaaaaagtgt tctgttaata aagctattga
    23281 tatagctata aaaatattaa gcaaatatga gtgcgagggg atgattagaa atgagtttaa
    23341 ttaagtatag aggttatgat tttgagaatg agaagtggat ttattcagca acaataatgt
    23401 ggagtgatgc agtagattgt ttatttatgc taaaagagaa ttgcgaatgg caaagagtat
    23461 gtaatgttgg agtatgctct ggagaatggg ctagaaacaa tcaagaaatt tgtgaagggg
    23521 atatattgaa agaatatgat aatttccatg atacaagtca atatggagtt gtaaaaagag
    23581 atgttgacag tattaaaata tatttagaat ggcattattt aaagaaactt gaaggagaat
    23641 ggatagagat tataaataaa acagaaatat atcatagcag agattacaaa gtagttggca
    23701 atgaatatga gaatttagag gaagttagaa aagagttttt agaacgcaag gagatgttag
    23761 aaaatgaaca tcttggctag tttaatactt atagtaggaa gttttatagc tggtagagtt
    23821 tatgagtata gattgaatct aaaagagtgt gaaaattgcg acaataaaag aggtgtataa
    23881 gaatggatga tagattagaa atgataaatg cttctgtaaa ttatatacag atgatatgtg
    23941 aaagttcaaa tatagctatt ataacagagc gaggaagagt tagaatatta gatttagaaa
    24001 ctaaagaaaa atatgattta ttaaaaaata aactcgagga aatgttagaa gaaatataag
    24061 tgaaaatatc taattaaaac agtttagaga gttgcaaaat gtcttttggt ataaattatt
    24121 gttgaagtgt tttgtgactc tcaaaaatga aaatagagag gggaataata taatggattt
    24181 aaataaaatt atgaatgatg cactagtgga aatagaagaa agtggctttg tagaggaaac
    24241 agttaaaaaa gaattagaag aaacaataaa aagagctgtg aatgaaatct ttggaagcta
    24301 tagtaaattc agaaaaaata ttgaacaaca tttaggtgaa aatataaata taaatttaga
    24361 tgaattagat atacaaaaat acaatttact tgtggcaaat gttgtaaaag aaaaggtaga
    24421 tgctacgatg aaggggcaag gtattgagca tttaaaaaga aacttggata acatgcttgt
    24481 aggtcttaag aaagaataca aaatgagtga attattagaa gaattgaaag aagataaata
    24541 caaccttgac gagtacccat gtgatgaaga ctgtataaca tttatccttg aatctaattt
    24601 taattcagaa tgggcaagat ggatagactt tgatgagtgt cctaatgtaa gcagaaataa
    24661 ttgtgaacat tctatactat taagaaacga tggaactatt gcagcattta gataccaaaa
    24721 cagagaaata tcatcaaaag atataatgaa cggatttggt aattttgggg atttattatt
    24781 taagatatat gcacataatt caaaaattat cttagattta gggaatgatg tagacgatta
    24841 tgacttaaca aatggagaag attattagaa taggggtgaa taaatgtcaa agtatgtatt
    24901 gagatggcaa aaaggattat tactagatga acgcaagata aattattctt gtggaagtat
    24961 agaaaattta aaaaagaaag ctgaattatt agctaaagat gacaagatat tatttataac
    25021 aatagataaa gttgaagaag ttataaaaga tactagaagt caaaaaatgg ctgaatatta
    25081 ttgtgatgga ggaattgaaa tatgataata cacaaattta taatacatgt tttagataag
    25141 aatagcgata caccaatact aaatgatttt gagggtaggg ttagtcaaga tattgaagct
    25201 ttctttcaaa agaaaataag caaagtatca agagataatg acatcagaac agcagtattt
    25261 aatgactata gtaacaatct aattaagaag tgttgtgaac aaattattta tgatgaaagt
    25321 tcatttttaa ataactctaa agagattgca gcttatttat ttgatgttat gaaattgaat
    25381 gctatattag aatcttgcga cttagcaatt tgcttatact ctcaaaaaga tgaaaagaaa
    25441 gttgctatat taaagcttga ttacaataat tcgtatactc attctattag ctttgaagat
    25501 gataaattta atatacaaat gtctaagaat gaaattaata tacaagagac taagacggtt
    25561 aaaattgctg ctttggttgg attgagtgga atgaatgacg aatatcatct aaaagtattg
    25621 gataaggatg cagagaagga agaagctaat tctaagtttg ttacagagtt tctaaatgct
    25681 actagagtga aagatgataa gtataggact aaaaagttta aagatacagt tgaaaattgg
    25741 ataactaatg ttcttggaaa tgatataaaa caagctgagg acataagaag tatattaaat
    25801 tatactttga aagaaaagca tgaaattgat ataaaagatt ttgttgataa atcaattaaa
    25861 gatgatgagt taaaaaatag ttttaaagaa catatggaag aaaagggtct tgatgaaagt
    25921 tttagcatag ataaaaaatg ggttgaaaaa aagcttaaaa agagaaatat aaaaactgac
    25981 aatggttttg aaataaaagg taacttaact gattttgagg acccaatgaa atatacagta
    26041 agacaaaatc aaaatgggtc tatagatata attattaaga atgttaattt ctataatgaa
    26101 aaataagggg gctacaatgg aaaataaaga taattttgta ttaaataagc ttggaccagc
    26161 aagtaaagta atgaaaaaag ttgaaataga tgaaaaagaa attaagagtt atctaaaaga
    26221 actaaaagaa tgtgaagaag taaaagtttt tattattaga aaagatggaa ttgaaattcc
    26281 aatagttttc aaagatggaa atatgaacga atggttaaga aaagaaattg agataagttt
    26341 tgatagattt aaggatgaaa ttgctcaagt taaaaaagca ttgattaaag ttacaggaga
    26401 gaattaagaa gttagaaggt gctcatatga ctaataaaga aatgtgcaag tcaaataatc
    26461 ttgatgaaag agaagtatat aaaagttttg gaaaagagat ttgtggcagt tgtaaaagta
    26521 ataaaggaga ttgtgaaagt aaaaattgta gtgaagcatg taaaaattgg ctagagaagg
    26581 tgtgtaaatc ttgactgaac taggagagct tattagagaa ataagggaag aagaaggcga
    26641 atacatatct gatatggcta aaagattaaa tattagttgt gaggatatat atgcaattaa
    26701 cagaggtaaa agagaattaa ataaaaatga aattaacaat gttgtaaaaa aatatgagct
    26761 agagggagaa catctatatt tgcttaaaaa tattacacat aaagataagt tatttgatat
    26821 agcatgcaat gaatgcggaa gtaaaaatgt agctatagga atgtttgatg tagcaaatga
    26881 catcatagaa tttagatgca gaaactgcaa tatgatagat gtagtaagtg tagatttcta
    26941 ttgagaagat ttaaataatt taaatagtca aagtgaagtt tataaatgaa actagaatgt
    27001 tatagactta ctttggctta taaaaggagt gcgttaaatg gctaatatat attgtgaaaa
    27061 ttataattgt aaaaactact ttgaagatat gtgtatgctt gaaagaattg aaattaataa
    27121 cctgaaagtg tgtgaaagtt acattgaagg taaaaatgag ctatataaat tagaaaacgg
    27181 atatactata catcctaaag atttgaaaat ggtgaaaagt aaagattatt ctgttgaagt
    27241 tactcatatt ccaactggta ttacagtaaa atgccgttct acaaatagta ttttaaaaaa
    27301 taaaaataag tgtttggaag ttctagaaga agaattaata aaaaactctc acttagagct
    27361 agaagattta cgctaaatag gaagtgattt tatgaaacga agaagatgca gttggtgtgg
    27421 caagttattt tattttaagg aaaaatctaa ggagatttat tgttgtaagg aatgtagaaa
    27481 gaaggctaag aaggtgaaaa aatgaaagtt tttcttgtaa tagatgggga accagttggc
    27541 aaagaaagac ctagatttaa tttggctact aaaaggacct atacacctaa taagactaga
    27601 gattatgagg gactaataag atggttatat caatctaaag ttaagtatcg ttttactggt
    27661 tatataaaaa tgactataag atgctattat tctatagcta aaagtaacag taaaaaggtt
    27721 aaagagcaga aaagaaataa tgtgttaaga cctagtaaga aacccgatat tgacaatgtg
    27781 gtaaagatta tagctgattc actcaatgag atagcttata aggatgatac acagattgtt
    27841 gaggttgtag ctagtaaata ttatagtgat aatcccaggg ttgaggttat attagaagat
    27901 gttatttaag gaggactata aatatgaatg agattatgac aaatgaaaat ttaagagttg
    27961 tagcagatga tttagttaca gtttatgaaa ctgatacagg agagaaaata gtttttgcaa
    28021 gagaactaca taataactta gaagttaaga gacagtttat agactggatt gaagatagaa
    28081 ttaaacaata tggatttaag gaaaatgagg attattcggt ttttcacaaa aatatgaaaa
    28141 actcaactgg tgggagacct tcaaaagaat atgtattgaa gcttgatgta gcaaaagaac
    28201 ttgctatggt acaaaacaat aataaaggga gagaaataag aagatatttt ataagattag
    28261 aaaagttgct caatagaacc ttatcaaatt cacagcttag tcaaattaat actattgtga
    28321 atgaatcatt acttaagatg gaagttaaac ataatatcca aatagagcaa cttaaaaaag
    28381 agtgttcaga atattataga ccaacctcta aaactaaata tgatatatct tcttatataa
    28441 aagaaagact aggtatatca aaagttaatg aagaatttga attagttaag aaaagaactc
    28501 tattggtatt aggtgctgat aaatgggagg atataccaaa agatgtatta cttaattcat
    28561 taaacttaat agatgaatca ataagaatta taaaatctga aagaaagaca agtcagatta
    28621 gtttctttga aaaagataat ttctgttaat aaaaagaaaa aaggagtgct ttcacactcc
    28681 acttgtcaaa aatataaaac ttttatatac aaatattatt ataacataaa aacataaaaa
    28741 attgatggga gtgtggaagt atgaataaaa agacactatt tcaagaggtt gaaggtagat
    28801 tatataacta taaaaaatta gaaagtcaaa taagaataaa agatatatat attaaaaaat
    28861 tagaaagtga gttttgcgca tgtaaggctc aaagttatga agaaaaaaca ggacctactt
    28921 ataatataag ttcaagtgtt gaaaatgaag ttattaaaag agaagaagat ttaaatagat
    28981 taaaagaaga taagaaaaca ttacagattg aaaaggaaac tatagaatgt gcattaacaa
    29041 gtctaaatag ctttgaaaca gagttcttca atgaaatgta tatgaacaat gagaaaatca
    29101 acatggatta tatgtctaat gctatgcaca tagatagaag tcattgcttc agaataagaa
    29161 aaagaatagt ttgtaaaatc atggatatgt tatatcccaa aataaaggag tttgaattgc
    29221 ctattttttc atggaaagct taaaaatgag actattttga gactttttgg agactatcgt
    29281 gagacttttt attgccaatt atgtgagata ataatatcgt ggaaataaag atttccctct
    29341 caaaactaaa taattgctag gttagtttaa agggctaatc tagcaatatg aacagactag
    29401 gcagggcgtg aggacgctgt tagttcaatt ctaactatgt tcaaaaccta ttaatacact
    29461 atatgtagta gttgaattaa gattaaaatc tcatacaatt ttgtatctta attcagaagt
    29521 ctaaaaaaaa cgggtggggc ttgctaacct cactcaccat gcaggtgcag gtgcttaatc
    29581 taagttcgat tcttagaact tgcgacataa tccctactaa aaagactaag cttacccaaa
    29641 cttagtcttt tattttttta caggagtatt ataatatgaa tgatttaaga ggaaagataa
    29701 taaaagaatt agatgaattt aataacaaaa tagtaaaaat taaaggtttt tatgagaaaa
    29761 gagagaatta tatctttatg tttgcataaa taggtaattt tgttataata tactaagaac
    29821 gaatgttctt ataagttgga ggatgttatg gataaagata taataaaaaa agtaaagttt
    29881 tctgagatag atattaatga cgaatttttt aaaagtttaa tatcagatta tattaatttt
    29941 gaaaaatggt ttttaaaaaa atataatgaa gaggcatata tccttaaaac aaatagaggt
    30001 ttgcaaggat ttttatactt aaaagatgag tatgatgaag actattcaat tagccccaac
    30061 tttgataaaa aaagaagact taagataggt actttcaaga ttaattctca tgggactgta
    30121 ttaggacaac gatttatttc aattatctta agaaaaatgg ttgaagaaga ttttaaagaa
    30181 gcctatgtaa ctttatttga aaagcaagat ggattaataa aactttttga gagatttggt
    30241 ttttcatatt ggggtaaaaa agataatggt gaattggttt atgtgcgtga tgatgcagat
    30301 aaatatgata tatatgaaaa tttccctaga attaaaacca caaatgttag aagttattta
    30361 ttaggtattt atccaaaata tcatacagaa ttatttccag actcaagact ttgcactgaa
    30421 aaaaatcata ttataaaaga tgtatcattt acaaatacag ttgaaaaagt atatttgtgt
    30481 aatatgagtg gaataatgaa tttaaaaaga aatgatttat tactaatata tagaactaaa
    30541 gaaggcgaaa aacaagggaa atttagttct gtggcaactt ccatttgtac tgtagttgaa
    30601 actaaacata taagttcttt ttctaattat gaagatttta tgaattattg tggaaaaggt
    30661 tcgatttttt ctcaagatga tttaaaatac ttttggagca agagaaagta tccttattta
    30721 ataaaaatga tatataattt acctttgaac aaaagaataa taagaaaaaa tttaattgaa
    30781 aaaattggat taagagaaaa agagtactgg ggattttttg agattaaaca agaacaatta
    30841 agaaaaatat tagagatggg tgaagtaaat gaaagtttta ttatcgatta aacctgaatt
    30901 tgtatatgaa attattaatg gaacaaaaaa atatgaatat agaaaaagta tatttaaaag
    30961 agagggtatt tcgtcaattg ttgtttatgc aactaaacct tatggcaaaa tagtaggaga
    31021 atttgagatt gataacatta tccaagacaa accttccaat atatggaaac aaactaaaga
    31081 attttcaggt ataacaaaaa agttttttaa tgaatatttt aatggaaaag atactggatt
    31141 tgcaattaaa attaaagatt ttatcaaata tgaagttcca ctagagttaa atgactttga
    31201 taagaatata aaagttgcac cacaatcatt ttgttattta ggagaagatt aatatgaaat
    31261 atatattctt agctggggtt catgctgttg gtaaaacaac acttacaaaa gagcttgaga
    31321 aaaaaataga tattgtatct tattctatta gtgatttgat tagaaagtct ggtaataata
    31381 ttcctaagaa tgagaaaaaa accaaagata tatccaataa tcaagtttta tggataaaag
    31441 aactacaaaa tttaaatgtt gaaaattcaa cattaatctt agatggacac ttcaccttat
    31501 taaatgaaaa tgaggaagtt tttccaattg attttaatgt atttgagaaa actaatatgg
    31561 ataaaattat attaataaag caaaatccta aagaaataaa gagaagattg ttaaatagag
    31621 atggaaatga atattcttta gataaaattg agtttcatca aagtaaagaa gaggaatttg
    31681 ctaaaaaata tgcaaaaact aaacagatac caatttatat ttatgactca aattctgatt
    31741 tagagtcttt ggttattttt attgatagtt aaattgagag gtgactttat atggtacatg
    31801 agttaaagat tgaccatgaa ttttttattc ctatattaga gaatattaaa acatttgaga
    31861 taagaaaaaa tgatagaaac tataaaaagg gagatattat catacttaag gagttaaatg
    31921 aagataaagc ttcttttaca ggtagatata taaaagcaaa gattacttat atatcagact
    31981 atcaacaaaa agataattat gttgtattta gtttcgaaaa agagctttaa ttcaagacca
    32041 atcaataata atttacaaat gtgtactcta gcaagagttc tttttttagt gaaaatttta
    32101 ttattttgta gttgtcgaat ggtttttgaa ggatattgac ctttgaagtt gaattttata
    32161 ctttggaggg gataacataa tgaaaaattt cataaaagaa gtgaaacttt ttatattaag
    32221 taacaagatt cttacactag ccttttgtat agcaacaata atatttgtat catatgaagt
    32281 tactaaggat ttgcctgaaa taattcctta tggagaattt atatttaatt tgtttagtca
    32341 actaagtttg gcttttatgg gatgttttgt tttctatata atgcaggttt atataccaga
    32401 aagaaagaga aaaaaagtaa ccaataatat tttagcaaat aaaattgaat acatattaat
    32461 aagaatgata gaacctgcac aatgcataac taattctatt ttaaaaaaga atcttgatat
    32521 aaataatatg gataaaacta aatttgcatc tttgtacaaa aaagattatt tagataaagg
    32581 tactaaaacg atatttagag gtggaaaaga atatacacat aaagattata taaaagaaaa
    32641 tatatgcgaa ataaataatt gtatttttga gatatatatg actattggtt ataatttgga
    32701 tatagatata cttgatatat taagtaaaat aagagactca aaatataata aaatctttat
    32761 aagagaatct gtgttagaac ctggtgtata tgattttaaa aagataaatg aaactccagt
    32821 aacagtaact atgaaagaag aaagcttcga taaagaaaaa aacagaaatg aaataataaa
    32881 ggaatactat gaattatatt taagtctaag aaagtataaa agaactttat aatagagttc
    32941 tttttttatt cccaaaacaa acaaataaag aggtggtgat gtgcaagatg tcaaagaaaa
    33001 ggtaaaacaa gattacttaa aaggaatgaa acaaaaggaa atagcatcaa agtatgacat
    33061 tagcttaaac actttaaagt catggataaa aagatacaac tggtcaaagg aaaaaaagaa
    33121 gggtgcacct ataaataaaa ggggtgcacc cttttctaat aaaaattcag ttggccatgg
    33181 tgctccaaaa gagaataaga atgctgaaaa gtttggtttc ttctcaaaat acttacccaa
    33241 agaaactcaa gacctaatta atgagataaa gaataaagat aaatttgata ttctttggga
    33301 acagataaca atacaatatg cagcaataat aagagcacaa aagataatgt atgttaaaga
    33361 caaagaagaa atgattaagg aattaaagaa acatgaaagc acagaaaatg gcgagaagat
    33421 agagtatgaa tttcaatttg catgggatag gcaagcatct tttcttaatg cacagagtag
    33481 ggctatgagt gaacttagaa gtttaattaa acagtatgat gaaatgattc ataagaattg
    33541 gaacttagct acagaggagc aaaagacaag aatagataca atgaggacta aagtagaact
    33601 tgagaaaatt aagttgtttg gtgatgataa agacgataat tcggaagcta tacaaagctt
    33661 cttagaagct actaccatga gtgaagaaga tataaaagct ttatttgaag aagaaggaga
    33721 gtgcaaaaat ggctctactt agaagaaaac aaaaaagaca agaaaagaga tttgaattta
    33781 aacctttttc aaagaaacaa ttaaaacttc tcaactggtg gagagaaggt tctaaatata
    33841 aggactatga cattatcatt gctgatggag caataagaag tggtaaaact atagctatga
    33901 tttgtagctt cttaatgttt acacaaacta attttgaagt agaaaacttc ataattgcag
    33961 gaaagactat tggttcacta aagaaaaatg ttattgaacc tatgaaacag atactaaatg
    34021 cttggggttg gcgatttgag tacaatcgtt cagaaaattt tttagtaatt ggtagtaata
    34081 catattatat gtatgatgct aacaatgaag cttcccaaga taaattacaa ggtttgacag
    34141 cagcaggggc tttagctgat gaagtagctt tatttcctaa aaactttgtt gaccaaatga
    34201 ttggtcgttg ctctgttgat ggctctaaaa tatttatgaa ctgcaaccct gcaggtccat
    34261 atcattttat taaaactgaa tttatagaca aagtaaaaga aaaattaatc tgttatttac
    34321 atttcacaat ggatgataat ttatctttat ctgagaaagt taaaaataag tttaaaagaa
    34381 tgtttacagg tgtcttttat aagagatata ttttaggtct ttggtgtcaa gccgaaggtg
    34441 ttatctatga tatgtttaat gagaagatac ataaggtact aactaaactt agagaataca
    34501 cagagcatta tgtatcatgt gattatggta ctcaaaacgc tacagtattt atattatggg
    34561 gcaaatgtaa agatgtttgg tatgcagtca aagaatacta ttacgacgga agaaaagaag
    34621 gaaaacaaaa ttcagataat aaatattata ctgagctagt aaatttttta gggaatatac
    34681 atcctaaagc tataataata gacccaagtg cagcttcttt tataactttg ataagagata
    34741 aaggaaagta tagagttaaa aagggtaata atgatgtact aaatggaata agaaatgtcg
    34801 gaactgcttt aaatagagaa atgattaaat ttaatgattg ttgctctaat atatttaagg
    34861 agttcttttc ttatgtttgg gatgaaaaag ctttagaata tggagaagat aaaccagtta
    34921 aagtaatgga ccatgccatg gatgcaatta gatattttgt tcatacaata ttatttggag
    34981 gaaaagaacc taattatgat gatgaaatct ataataaagg cttaggattg aagaaaaata
    35041 atatacaaaa tcaatataat aagaaaggag ggactgtatt ttagtgaata taaaaaatat
    35101 cttactcaat ttagatgaaa aagagttaag agatagaaaa catgcagaaa gagatttttt
    35161 gttttatcta ggtgaatgta gaaataaaac tatgggactg ttagatgatg attttttagg
    35221 tcaaagttgg attacttttg ataacctaga ctatacacct tctcaaattg tggataataa
    35281 ggtaaaacca cttataaata aacaagctcg ttttatgttt ggaaaagagc caactataat
    35341 acttaaagca tatgaaaaag aacataaaga agcatgtgag gaattaaggc agtatattga
    35401 ttcaatatta aatgccagca agttttggag caacacacta aaagctttta agatagcaac
    35461 tataacaaaa agagtcttat taaggttaga agctgaacca aatcaaccta taagactctt
    35521 ttatcattct ataaatgatt tcaaatatca agttgatagt aatgatatta caaaattaaa
    35581 gtcagttgta tttgttagat ttgattcatc aactataaaa gaagtaacgg caaaacaaat
    35641 atggtataga tatacttact atatgaaaaa aagtaatgtc agtaatcaag aaagctgctt
    35701 tataaagata gaaaagttca aaggtgataa cttatctaaa cctattgaag taaaagaaag
    35761 tgataccaag ctttctaaaa taccatgttg ggtaattgtt aatgaacaaa gtattacaaa
    35821 tatcacaggc attagcgata ttgaggattt aaagccatta caagatacct ataataaaag
    35881 attatcagat ttcaatgact ctttaaaatt tctgatgttt gggcaaactg ttgtagtaga
    35941 tgcgacagaa gaaacagtta atgcttgtaa gattgctcct aatgctttaa tggcattaaa
    36001 aacacttgaa gaaggttcag aaaaaggaaa acaagcacaa gcgtataggg ttgagagtag
    36061 tttttcaaat gcagaccctg taaactcttt ttttaaaaga cttgaagata gtatgtatga
    36121 aaaattagcg atacctagac ctgaacaatt acagaatata cctagtgcta aagctttgaa
    36181 atatttatat acagagctta ttgcaagatg ttcagaaaag tggaatgatt gggaacctgc
    36241 aataagaagc atgttaagat taatagttga agcttgtagt aaatttaatt gttatgatga
    36301 ttggaatcat gattgggatg atttaatgtt ttctattgta ttaaataaaa attatccaat
    36361 tccagaagat gaagaagatt caaaaagatt ggcacttgaa gaagttaata ataatgttag
    36421 aagtcataga aattatataa aagaatttgg agatgatgaa gattatgagg aagcatttaa
    36481 tgaagtacta gaagataatg aaaagataca atcagtagag caagaccagt tcagaaaaga
    36541 tgcagaaata gaaattgata atattgatga agaattaaat agtaaatcta ataatagtga
    36601 tgagtaatga ggtgttgtta tgaaggacaa tacttataca aagaaagttc tcgaagctag
    36661 aaaaaaactt ttattattag ataaaaaagt acaattagag atattaagtg tctataaaga
    36721 tgctagtaaa actattttaa gtgatattgt taaaaataaa gagttgaatc taagtactaa
    36781 atacttaaaa aagctaaata aatcaatcga gaagtatatc aatgagctaa atcaaagatt
    36841 agtacctgtt acagaaaaaa gcataataga agcttctaat atagctaaag aattacaaat
    36901 gtattactat caatcaatag ttccaaacga atctataaag tttgcatgtg atgctatgtg
    36961 tataaagaca actactagtg tggtggaaaa actagttgca agtaactttt ataaagataa
    37021 aaggtcatta gatagtagga tttggggcta tagtaataaa aatagaaaag atattgatag
    37081 attaataaaa gctaatgttg caagaggtgc taatgcaaag actttagcaa agagtttaga
    37141 taattatgtt aatccagtta aaaagacaga agctaaaacg ttagaagttg gaatgaataa
    37201 aagtatatct tatcaagctc aaagacttgc tagaacttct ataacacatg cttttgtaga
    37261 aacaagtgtc caaaatgcaa taaataaccc catgtgtatt ggtctacaat ggaacctaag
    37321 ttctcaacat tatattagac aagtaaaatg gaggggcgag gatgaatgcg atgaatatgc
    37381 agaacaaaat agatatggat taggtgaagg agttttccca ccagaaaagt atcctattcc
    37441 acaccctaat tgtctttgtt atcccactca agttatagtg ccaatcaatg aagcttctaa
    37501 aatgatgaac gattgggcaa ctggtggaga taatgatatt cttgatagtt ggtttgatgg
    37561 aataatacat caaaataata gtattccagt cattaagaaa caaataaata aaaagaataa
    37621 aataactaca aatagtaaag atgatagaat taaaaagaac cttactaatt caattaagaa
    37681 taagtcaaag aaagcaccta aacagattca aaaatatata aataagtatg taaatacaaa
    37741 taaaataata attgataatg gtcaaaaaat accttttgtt tattacacta aggttgattt
    37801 agttggtatt aatccaaaca tcagggaatt taaatcttat aataaagaag ctgctttact
    37861 tcatgagttt gcacatagaa ttgatataaa agaaattaag agctataata atattaagtt
    37921 ccaaaaagct attgaaagta gctctatata tgttatggag aatattgaga acctgcaaag
    37981 tatatatact aattctaatg agttgtacaa caatgaattt attagtgata tattaggagc
    38041 attatctaat aatgaatttg aggatttatt agcaactcat agcgaaaagt attggagtaa
    38101 aaacagaaat aaagaaaaag aaatatttgc taatttattt acactaaagt atcaaaataa
    38161 taaagaaata aatagtttta ttaaagaaca tttaaatagt ttagataaaa tatttaatga
    38221 attgctagga gggatttaga ttgcttgaag aaatgagaaa agataaaaag ctgttagagt
    38281 tgagacgatt atataaagag aagtatggta agaacgcacc tgggttcaac tatgatgaat
    38341 acaacagtta tgctgagtac aaagaaaagt taaaagaatt gatacaaaaa taaagtgagc
    38401 acttattaat taaatattaa tgagtgcttt tgttatgctt aattttagga ggaaattaaa
    38461 tgttagagta ttttaaaaag ttacttggag atgaagaagg acaaaaagtt tatgaaaaat
    38521 tatctaaaga taaagaaaat aagcttcttt tagataatat taaaagtcct aggtacgttg
    38581 aaaagacaga gctagaaaat gctaataaag agattaaaga gtataaaaag caaataggtg
    38641 atagagataa gcaattaaat gatttacaag gcaaagttaa agataacaaa gagttatcag
    38701 atgaaattga aagtcttaaa aatgcaaata aagaaattag agaaaatgca gaaaaagaaa
    38761 tagaggtttt aaaatttaat acagcttttg atagagctat tgaaagttac aatcctagaa
    38821 acccaaaagc tttggcagct ttaataaata aagagaatat taactttgta gatggtaaaa
    38881 ttattggctt agatgagcaa ataaaagctt atcaacaaag cgattcttat ttattcaata
    38941 acgaaaaaaa caaaggaggt gatgaaattg gaggaactgg aggactagaa ggtggaacaa
    39001 catcattact tgataatgat agcgaaataa gtagtatagg tgagttgtta gctaactcta
    39061 aagtaaaaag tgaaaatata gaagctcaaa agaaattctt tggagaagaa taggaggaaa
    39121 atatatgagt atagaaaaat ctgagattta catgggggaa aataaaacca tattaaaatt
    39181 tgcaggaaat ttatttcaaa atgtaaatat taaagtgaaa aaaaccgatg tagctatagt
    39241 agatgaaaag agaattttaa aagcaggtac attaatatca aaagatggaa aattggtaga
    39301 tggtacaact gttacaaatg ataaggcttt tggtttagta tatagagata tagattttac
    39361 ttattcaaat gggaatgaaa gtattccagt atttatattt ggatttatag atgaaaagac
    39421 cttgccaaca gctataccag aagaagcaaa acaagcaatg aaaatgatta tgtttttata
    39481 aaataaatat taggaggaat taaaatggat tggaaagact ttatagattc taaggagata
    39541 gctaaatata taaaaaaatt accactagaa atgttaatag gcgaagcttt atttccaaga
    39601 aaaaagcaaa taggtatgga cctaaaatat attaagggag cgaagaaaaa accagtagtg
    39661 ttaaaacaaa gtacttttga tgttgctgta aaaataagag cattaaaagc tcaaatagaa
    39721 gttaaatcaa agagaatgcc attttttaaa gagagtgttc ttgtaaatga ggaagacaga
    39781 caacaattgc ttttagcttc acaagctcaa aataaagaac tgttactaat gataatttca
    39841 caaatatatg ataattattt agctcttgta gatggtggcg atatgcaaat ggaaagaatg
    39901 agaatgcagg cgctagctga tggagtaata aatatcgtat cagaagatgg agatttagta
    39961 tttgactttg aagttccaag caatcataaa gaagtgttaa ctggaagtgc aacatgggat
    40021 aatccagatg cagatattat aggagatatt caaagatgga tgagaataat gagagatgaa
    40081 gggaatccgt tacctaagag aatggtaatg acaagtaaaa cctttgggta ttttgctaaa
    40141 aataaagcta ttaaattaga tatagataaa gatggtagag ttattttaac tgatgaaatg
    40201 attaaaaatt atcttaaaaa taaagttggt ttatctgttg ccatagtaag tggaacatat
    40261 aaattagaag atgaaagtga agaatcttat ttccctgata acaaaataac ttttattcca
    40321 gatggagatt taggaaaaac ttattatggt acaacaccag aagaagctga caaagtatat
    40381 ggttctaaat tagattgttc tgttgttaga actggtattg ctataacaac aatgagatta
    40441 attgacccag taacagttca aactaaggta tcacaactag gtatgccaag ctttgaacgt
    40501 gctgatgaat gtttcttcgc tacagtagca taattaaggg ggtgataatt atggctaaaa
    40561 agaaagataa tttaatgcaa gtaaaagctt tggtatatct aaagtatgat aatggatgct
    40621 ataaaatagg tgatgtattt gagattagaa aagcagacca agaatctatg gaagaaaaag
    40681 gatatataga agtcatagga gaaattgaag aaaagaataa tagtgataat gagcttcttg
    40741 aaaaagatgg tgagtagata tgtctattac caatttagat aaattaaaac ttaatttgca
    40801 agaagaagag tatccttatt ttacagatga acaacttgtg atgttattag aatctaatga
    40861 gaataatgtt ttaaaagctt catggagagg ttgtttactc aaaggtgcta cagatgattg
    40921 tattaaaata ggccctatag aaactaagag tagtaatagt tcatactggt tatcattagc
    40981 agatatatat aaaactgact atttagaaga aaaatcaaag aatgaaacaa ctaacacagg
    41041 atataaaaca tctatgataa gagttgatgg gcaatgagaa aattaagagc tgataagata
    41101 ataaagacta tcaatagagg aataacttta aatcctcaaa caataactat agagcaagaa
    41161 gtgaaaaata tagtagatgg ggctattgag gttacaaatg aagtaaaaga actaacagta
    41221 gttatatatc ccgaaaaaac taatgataca gtaataaata gtgaaactat tggcacagct
    41281 tataaaaata aaaactttgg tatggttaca gataaagaag ctgatttaag attgaatact
    41341 gaaagtaata taacatttaa atgtattgaa ggtactatga aattaaacta tgtaaatcct
    41401 gttgtagttg aaggcaaaat ctgtggttat atatgtggtc ttgaaaagct agattagagg
    41461 tgatttaatg agtgtgttta ctaaggctat aaatgaaatt gataggaaaa agtctacaat
    41521 gccacttctg tgtatgaata tagcttttat gttagaagga gaagctaaaa atagtgcaaa
    41581 atggactgat aggacaggaa atgcaagaca aggtataaca ggaactagct taggtggagg
    41641 aaatcaatac attgttaggt taggacatgg agttgactat ggaactgttt tagaggaagg
    41701 ttcagcacct catattataa aaccaagaaa tgcaaaagct ttgttttgga atggagcttc
    41761 acatcctgtt atgaaggttc aacatcctgg tactaaaggt acacattctt tagagtccac
    41821 aattagtagg aatatgccta agatagggaa attaatagaa ggacattgga gtaaataata
    41881 tgagagcagg aataagaaaa gccttaattg agaacatacc aaagcttaaa gattgttatg
    41941 aaccaactgt acctaataaa aagactataa aaccatatgc tgtaattgtt caaggtgaag
    42001 atgctgacaa cgagggtgat gttataggtt ttagaaggat cataaatatt tggttatatg
    42061 agaaaaggac tacatttaat aaactagatg aacttacgaa agaagtcata gaaactttag
    42121 attttaaaac tataacagat aatacatcta acgaggtatt tacttgtatt tatgaaggtg
    42181 cagttggtca agatgttata gatgaagaat gggaagctat aataaggtgt ttaaggttta
    42241 gtgtaatagc tttagatgac aaagatgata taactagtga tagatgggta gaagctttat
    42301 ctaagtacac aaaagattta ttagaaatcg agagttacaa agataattgg aagaaaaact
    42361 ttatagcacc ttgttgttta tggagaacta caaatattga aaataaaaga attaactatc
    42421 atttaatcga gattactaaa actatgaaat gtcatgttgt tagcaaaaat aaggatgaaa
    42481 ttattaagct tcttgaagca ttggaaacaa agttaataat agataaaaga gtaagactta
    42541 gagaagataa gaacatgtat ttaactcttg ttagcgtggt tgaggatagg gaatcagata
    42601 tgtttacaac tggacaatta acagtcatat ttaaaatgat aggaaaaata aaaagagaag
    42661 gacctattat gaatgcgatt tataatagtg gaaatttgag ataggaggtg caaagattgg
    42721 ctgaaacaaa taataaaaag actaatgtaa gtaagcagga agaaaaatat ttgaaaagtg
    42781 attttataaa aaatagcgaa gcacttggct acaagaaaga agtagttgca ggtgctttat
    42841 ttaattgtaa gaaagatgaa cttacaaaat cagagtttga gaagcttata aaagatttct
    42901 tagaaagaga ggtgaagtaa aatggcaact ggtacatgga atgaaaaaga aaaaaaggag
    42961 atacctggtt tttataacag gttcaagact caagcagaaa agtctacaaa taaaggatta
    43021 aagggtagat tagcaatacc agttaaggct aattggggag aagttggcaa ggttgtaaca
    43081 ataaaaaatg acttgagaca gcttaaaact ttatttggtg atgatatgaa ctattcagca
    43141 tataagttag gtaaattagc tttattaggg aatgtaaagg aattactttt atataggctt
    43201 gtagatggaa atcaaaagaa gggtacatta acactaaaag atactacaga aaatactgca
    43261 aaagatgtaa ttaagctaga aactaaatat ccaactagca gaaattttaa tgtaacaata
    43321 aaatctaatc tagtagatgc agataaaaaa gactttgtat tcttcgaagg aactaaacag
    43381 ttatttagtt caagtgttaa aggtactata gatgaaatag tgctagaaat aaactcaaat
    43441 ttagataatg agtatgtaat tgcaactaaa gtagctgata gtgatacaac attagcaaac
    43501 ttagtaaatg tagcacttga gggtggtaat gatggttgta catctataac aaatgaatct
    43561 tatctaaaag cattagaaga atttgagaga tatagttttg attcttttgt acttgatggt
    43621 gtagctgacg aagctttgca agaaacaaca aaagcttggg tagctaaaaa taaagaatta
    43681 ggaaaagaca tactactttt tctaggtgga aaaacagagg ataatataaa acaaattaat
    43741 gataaatcaa aaggttttaa tgatgaaaat atagttaatg ttgggagctc agcttattat
    43801 gaaggaataa aatatacacc tagcgaagta gctgtttata taggagcatt agcagtaagc
    43861 aaaggtataa caggaagtat atgtaatgct aagactatat ttgaagaagt agaaccaaga
    43921 ctaagtcaat cagaagttaa agagtgtttg aaaagtggta ctttaatctt agactttgat
    43981 gatggagatg tgattatagt tgatgatgta aatacattta aaaagtatac agatgataaa
    44041 aacgaagcta tgggatatat atctaatatc atgtttatta atactataaa taaagatact
    44101 tctctaaaaa gaaaagaatt tgtagggaaa atatttaacg attcagcagg tcaaacaact
    44161 gttatatgtg cattaaagaa atattttgaa gaattgatga gtcaaggtat tatatcagaa
    44221 tttaatgttg atatagatac agaacttcaa gcaactgcca aagcagatga attttattgg
    44281 aaatggaatg ctgttaaagt tgatgtaatg aagaaaattt atggcacagg gtatttagga
    44341 taaaggaggt tgtagaatat gtataatgat gattatatag aagaagccag ttttttgaat
    44401 ggttctgatg tggttatact tattgatggt gtagaagaat tatatatgga agaaataaag
    44461 gctgattttg aacaagatga acaaagtata aaattgttag gatgccaaaa cgaaatatca
    44521 agagttggta ctactaaagg ttcattctcc ttgaatggat ataagacaga ttcaagattt
    44581 gcaaaattag gatttaggtc ttttgaaata atatataatt tatctaattc tgaaacatta
    44641 ggatatgaaa gtattagatt aaagaattgt agattaaaaa aattgcctct tataaattct
    44701 aaagctggtg aaattgtaaa aatagaagta gagggaagtt ttagaggata tgatttgtta
    44761 aatgaacttt aaaacaaaat agtgttgtac tttaaagcta taagcaatta attttgtttg
    44821 tagcttttta aaattaaaaa tattggagga atagtatgtc agagatatat aaaagagagt
    44881 tagaaaatga agtagtagaa gaagataatg atttagatga agaaatagaa gaaacaaatg
    44941 aagatagatt gaagatgaaa gaagatgaaa taatagcaaa gctattggag gattcaccag
    45001 tacctgagag aactgtgttt ttggatagat taggaatacc aatcactttg aaagcattaa
    45061 cagaaaaaga gattagtaaa ataagaaaag attgcactaa aattgtaaag gtccaagggc
    45121 gaagagaaga aaaattaaat gatgatgagt ttactctagc attaatagaa aaaggaactg
    45181 taaaacctaa tttttctaat caaaaattac ttgatgctat gaaagtaaca aatgctagag
    45241 aatttataaa gagaaaattc ttagctggag aattaagcaa aataagcgac caaatattag
    45301 aattatcagg tttttatgat gaaataagtg acgatgatat aaaaaactaa taaaaatggg
    45361 gggacgcctg actgttttaa ataacatctt tgttaagcat catgttcccc cagatattta
    45421 tgttaagaaa aatttaatgt ctcaacgctt aatgaaagta tttactcaaa atgaaataga
    45481 gcaagagaat aaagctatga aaaaataaat atctgtagaa aggtaggtga gggaaatagc
    45541 taaaaaggaa atgtatcata ttgatgttgt cataagtgca aaaggtgacg gagaaacaaa
    45601 aagcaaacta agtgttatgg aaaaatacat gaagcagaca gaaaaaagga tgcagacact
    45661 taatagaata aaggttaatc ctgctataaa agctacagat aaagcttcaa gtgttgtaaa
    45721 tagagttaat aacaatatga ataaagcaaa aaaaactgtt acagcaagaa taaaggctac
    45781 agataatgcg agtccagtag ccaatagggc tagcaataat gttaataaag ctagaaagac
    45841 agtaacagca aggttgaaag ccacagataa tgcgagttct acagttaaca aagttaataa
    45901 caagataaaa gaagttgcta aacctgtacc gcctgtaata attagaggac aagacgagtc
    45961 tagttctata atagataaag taaaagctaa gattcagaac ttaaaagctg atactattat
    46021 aaagataaaa tctcaagctg atgaagctat aaatactatt tctcgaacta aaaataaatt
    46081 acaagaattt gtgagtaaga gatatgaagc agcagttaaa attagggatg aagctagttc
    46141 ggttttagga gggttaacag ggaaaataga ttcttttgta aatggagcta ttagtaaatt
    46201 tgctagactg gctactaccg caggagcttt aataggtgga attggggtag gttctgctgt
    46261 aaaaggattt gctacttttg aacaaagtat gaagaatgca caagctgttt caggagcaac
    46321 aggaaaagaa atggaagctt taaccgcaaa agctagacaa cttgggagag aaacttcatt
    46381 tacagcaaca gatgcaggga acgcttttta ctatatgggt atggcaggat ggaagtctga
    46441 acaaatgata aaagcgatac ctgatgttct taacttggca gcagcaggag gaaccgattt
    46501 ggcactaacg tctaaagtat attggacagc tatagagaga tttatagtaa aaaatatggg
    46561 gttaaaatag ggaagcctaa gtccttaatg gatatggtaa tctattgcca ctttatttag
    46621 aaataggtaa aaggcttaac gactagatta gtaatctaag tccttaatgg atatgatgaa
    46681 agtccacgaa atcccacacc ctaacgtatt aggcgagggt gatgaaatag tctgaactat
    46741 atagaaatat atagaagcta agataaagag cttagtgtat aacaaaatgg atatagtgac
    46801 ggatggttta actgcgttgg ggttaacggc aaatgacaca actgaatttg tagatgtaat
    46861 ggcggcaacc ataacgaatt ctaatacaag tgttgaattg atgggtgaga catttaaata
    46921 tgtaggttcc atgggtggag ctttaggagt atctatgaaa gatttatctc ttgcgacagg
    46981 tttgatggct agtgcaagcg ttaaagggag catggcaggg acttcgctaa gaggaggtct
    47041 agttagatta ataaagccac cagcagaagc tgcttctgca attaaaaaat atggaataga
    47101 attaaagaaa aataaaaatg gaagtttgga tttagcagga acaataggaa gtcttagaga
    47161 aaaattaggt ggattaggag atgttgaaaa aggagttgca atatcttcta tatttggtcg
    47221 tacagctatg gcaggttggg cggctgtagt aaatgctagt gaaagtgatt ttaataagtt
    47281 aactacagct attgcagaaa gtgaaggaga agctaagaga attgctgaca tgaaactaga
    47341 taccctgtca ggacaatttg aacttttaaa aagcgctatt gatgatgtta ggataagtgt
    47401 aggaagtaaa ttaggtccta tgactcgcca atttgtagaa gatttaatta agaaaatgcc
    47461 acaaatcggt gatgctatag ttggagtagt agaaaagttt gttaataatt tcgataaaat
    47521 aaaagcagga tttcaagtat tattgcctgc aattggttct gttatagggg ctattatagt
    47581 tctcaaagga gtttttgctt ttggaagtgc aatcaagagt ttaagtcttt tagctaatgt
    47641 ttttggaaca gctaaattag ctgctcttgg tttgacagca ataataggag ctgtagttat
    47701 agcttttgca ggaatgacag tcgcaataag caataacaaa attgctataa tggatttaca
    47761 aactaggttt ggttcatttg gcgagtatat tactacaata atggagagca ttggtggagt
    47821 cataaaactt acattaggta acttgcttat aatgttaggt ggaattggaa aaggaatagg
    47881 catattgtta tctgataaga gttgggatga aaaagcttca tcattaaaaa atctatttgg
    47941 taaaacaaca gcagaaatta aaacaaatac aaaagaagct ttatcagata taaattcaga
    48001 aacttctaac gcaacagctc tattaaaaaa gtctacttca aaagagttac aaggtgttac
    48061 taaagctttc tcaacagctt ttgaccaatc taaaaatgta acagagaaga aatctagtga
    48121 tatagctaga gcattaacaa atggattaaa aggtttagat gaccaatctc ttactatgat
    48181 gcgaggatta aatgataata tggcgataat tttatctggt gtgacagcag atatgaaacc
    48241 tagtgacaaa gttaacaaaa tcaccaaaaa tcttgatgat gctttcaaag caggtaaatt
    48301 aagtgcacaa gaatacagaa gtagtataca agaaacttta agttttataa gtaaatatag
    48361 tgcagattct tcaaataatt taaaacaagg catgagtgat gctttcaatg ccttcaagga
    48421 agggacaaat gtaggtggat taaaagacgg agtaacagga atgttgaact ctttgaaagc
    48481 gacaggaccg caggttttag aaaccttaaa aagtttagga ggaaaagcaa gcgaaatttt
    48541 taagggagtg gattttaatt cttctataga tgcacagaaa actaaagtat tacagaatct
    48601 aaataatcta ggtttggaag gaacacaagc tatagataca ttaagaacta tcttctcaca
    48661 ggcttctact gcactagata ctacaaattt gaaacaaggt ttaagcgata catttaattc
    48721 atttaaaatc ggatttaatc aagatgggat aaatggagca ataaatagta tgcttggaac
    48781 gataaatcaa ataggaccac aaatgcaaga agctctaggc aaaatggatg gaaagatggg
    48841 gcaagtattt gctaatgtag actttagcac cccaatagaa acacaagctt ctaaagtttt
    48901 ggaaaatcta aaaaatttag gaatagaagg accacaagct ttagaaactg taagaagtat
    48961 atttgctcaa gcttcaagtc aaatacaagg ttcagcttct caaactgctc aacaagcaaa
    49021 tcaacaggtt gcaaatgctc taacacaaga aaacccagct gtgcaacagg cagggcaaca
    49081 attaggtaca gatttgacaa atggtgtagt aaatggaatt caagcagggg tgccagccgt
    49141 tcagcaaaaa gggaatgaat taggagtagc aactcagcaa ggtgttacag atgctttaaa
    49201 taatgcaact cctcaagtca ataattctaa ccttacaact ggtatagata cggcatttaa
    49261 ccaagctact gcaacagttc aacaaggagc gacgaatatg tataacggag ctaaacaaag
    49321 ctttagtcaa ttagctcaag ttggaaaaga agcaggaagt agcttgtata atggtgctac
    49381 aacttctttt aatatgttag catcaaatgt aagggttgca tgtagtagca tgtacaacgg
    49441 agctagaaca tcttttacga gtttaagtag ttcagctata agtgtcattt ctgctatgtg
    49501 tagttctgta gtaagtcaag ttagtgctat gtcaagtcaa gttataagct attggaatag
    49561 tgtaagagca acagtttcag caccaataag tgcatctttt aatgtcaaga ctacacaaac
    49621 aacagttaaa aggacagtaa atgaaagtag tggtggaata ctaagtaata tatttggtcg
    49681 ctttgcagaa ggaggagttg caagtaaacc atctatatgt ggagaagatg gagctgaaat
    49741 ggttattcct ttatcaaatg ctagaagaaa tcgagcaata ggcttgtatg aacaaacagg
    49801 taaaatgtta ggattaggct caactccttc tcaaaaagta gtaggtagtt ctaatgttgt
    49861 aaataacgtt agacaattta caaatatttt ggattcagac aataaagaat ataaagaagc
    49921 tcaaacacag agtttagttt caagtaatgg gaatacaata aatttaggtg gaatatctat
    49981 caatgttcaa aatagtgata ataaagaaga aatgatacaa gaaataatgt ctcaagtaga
    50041 aaatgaacta agagaagcat tgcaagacat tggataatgt cgaattgttg ttaaaaaaat
    50101 cttcctcata aatgatataa tattgttaaa atatgcaaat ttatgagggg gaaattttat
    50161 gtggcagaaa tttaaaagtc taaatacttt cttaaaagtt attataggta ttatagcaat
    50221 atgtttctta ccaatcactt tagctgtttt ttcaattgaa tttttaatga aagctattga
    50281 agctaaaaat aaaagcaaaa taatcttagg atgtttttta gcattattaa cattaagttg
    50341 tgctaggact gtttatctta caagagattt agatgataaa aattctactc aagtcaatca
    50401 agtttctagt aataaagata aagaagatgc tgaaaagaaa attaaagaag aaacgaaaaa
    50461 acaagaggaa gttaagaaaa aagaggaaat agagaaaaat tctaaagagg acaacaaagg
    50521 aaaagtggaa acaaaaaaac aaacattaac tggtgaagag ctaaagaaaa aagttgattc
    50581 tataattcca gcagagtata aaggaaatta ttacacaaat gatgtattag atacagatgg
    50641 tagctatgta cttagtttac aagttcaaaa tgcaagtttt gacaacgaaa gcagttgtaa
    50701 ggtatttact aaagacttaa ttaacaagtt gaaagaattt agaatagact cagcagaaat
    50761 atattttgta ggttcaagtg gtcaaacaac atatcaaatt aatatagatg atttcttgaa
    50821 agttcaagat aatgttaata gcgttgataa catggaattt ttctctttca aagactttaa
    50881 aaattagata gacaagaaaa aggcatttat ttattaggaa tagatgtctt ttttattgta
    50941 aaaaggaagt gataacttgg taatagacat ttatctaaaa aatgaaaaag aaaagataga
    51001 ttttcatttt ccagtaaccc cacaagattc tctatctata aaaaaagaaa aaagatttga
    51061 aactgtagat atagtaaatc taggtgaatt tgacattaaa aaagaagggg agaagataag
    51121 agaaatatca tttaaaacat ttttaccttt tcaatatgac gtttcttatt gcagatatag
    51181 tgagttaaaa aatccaactg aaacagttgc aatgcttgaa aaatgggtag accaagctga
    51241 acctctaaga cttatcatga cagggtttgg ctacaatgga ttagttacaa tatctagttt
    51301 tagtaatacc caaacagcag gaagagaaga agacagagac attgagataa catttagaac
    51361 ttacagagaa ctaaaaatag agacattaaa aaaagaaact aaaagtaatg ctaaaacaga
    51421 tttaaaggac aatagaccta acactcaatc atcatcaaaa acatatacag ttacatctgc
    51481 agacacatta tggagtattg caaagaaatt tttaggtaag ggttcaagat ggacagagat
    51541 ttataatata cccgaaaaca aaaaagtcat tggtaaaaat ccaaatgtga ttaaaaaagg
    51601 acaaaagttg gtgatacctt ctaaatgaaa ataatattaa acggaaaata tgatattgca
    51661 aattttaacg agggaataac tctctcagaa gctatagatg gagttgcata taagatggat
    51721 atatcattag tagaacctaa acaacttcaa gatataggaa ttaaaaaggg tgataaaata
    51781 gttctaattg atatagcata tgagagtaaa aaagaagaaa caatcttcaa tggagtcata
    51841 tgggaaacta ggagaagtga aaagagtaaa aaactgacat tgtcttgtag agaaagaaca
    51901 gtttacatgg aagaaagtga agaacaatac tcgtttaaag aaaatacagc gacacagagg
    51961 attgagtact actgtaaaca atggaatata ccttattaca acttagctaa tacagggaag
    52021 aaacttgcta aagtaataca taagactaat atactagata tgattaaaaa agacttaaaa
    52081 gaaacagcaa gtaaaggtgg agacttattt agagtaagga tggataataa gttgaaatta
    52141 tttaaacttg gtactaatgc aaatgtatat aaattagata gtatattaga agatgcaaac
    52201 tttacaagta gttttaatga cgcagtaaca agtgtaaaag ttttaggtaa gagtaaagat
    52261 gaaaatacaa aagcacctat aattggaaca tataaaaaag attcagacaa atatggaaca
    52321 ctacaaaaga ttaaacagga tgaaaagata aaaaatgcaa aagaagctaa gaaagcagca
    52381 gaaacgatgt tcaattcggg agaagaaaca ataagtgttg attgtgcagt agatataaat
    52441 agaataagag caggtgataa ggtatctcta aaaggtaaag agtactatgt tatagatgtt
    52501 actcatacgt tagattctac accgaaaatg aagctcaata tagggacttt agaatataca
    52561 aggaggaagt tttatacaaa tgactgatgc tagatttaat ggagttgcta gaatattgaa
    52621 agaaaaaatg aataaaagtg ttaatgatgg agtttttgga ataggatgcg aacttgcaga
    52681 aataacagca aatgggctta aggttaatgg ttataaagat gaaatacagg attatctagt
    52741 attagagaat ttaactttga aagaggatta ttttactctt tcagatgaaa tgttaggtgc
    52801 agaatataga cataaacata aaatagaaac tccaaaagaa ttaaaaccct tggctatagg
    52861 cgataaggtg ttagtagccg ttatgggagc tgaatttgta gtaattggga gggttgtaaa
    52921 tgccaaacct atttcctcaa aatgaaactt ttgaaactat agaattaaaa aataataatg
    52981 aaagtgaact ggacctaaaa gggtcttttt tatttgattt tgaaaaaggc gaatttgtta
    53041 aaaatgcaga tggaacatta aaaaaatgtg acaagatgca ggcatataaa caatggtgtc
    53101 aaaaggctat actaacacct aggtacaaaa ggtcagctta ttctagtgta tatggaagtg
    53161 aaataaaaga cttaattgcc agtaacttat ctcaaagtgc aaaagagctt gaaataacta
    53221 gattaataaa agaaactctt ttagtgcatc cttacacaaa agaagtaaat aattttacat
    53281 tcaattggct tgaaaacagc agacttgtta attatgaatt tgatgtgctc acaaaagatg
    53341 atgaaaatat agtaattgat ggcaatataa aaaggtaggt gattatatgg aaagagagct
    53401 acctatacca agcttcttaa cagaagatga agacgctgta catgaaagga tgctaagtaa
    53461 ctttcaagat gtaagtacat tagaaggtga ctttatatat gactcaacaa gacctacagc
    53521 agaagaaatc acacggctaa aacagttagg attacaaaat aatttaaaga ttgcatttcc
    53581 tcaaacatct tatggaactt atttagaatg gttaggagaa tgtaaaggag tatttaaaaa
    53641 tcaaccaact aaatcggttg gagttattac ttttataggt atacaaggaa ctataattac
    53701 aaaaggaact atagtaacta ctgttgcaac tgatgaaaaa caaagcatag agtttgagct
    53761 tcttgaaact aaaactatag gagaaagtga gatagtagat attaaagcag aatgtagagt
    53821 tgcaggaact atagggaatg tgtctaaagg tagtgtatcc gttttactag gttctattag
    53881 tggtgttaaa tctgttacta ataaggaaga tttcaaaggt ggaacagata tagaagatga
    53941 agaacatttt agagaaagag ttcttgtagc agagcaagag gacaaattaa gtggagctag
    54001 ttcagattat ataagatggg ctaaagaagt taatggagtg ggatatgctt atgtagtttc
    54061 agaatgggct ggaccaggta cagtaaaagt attaatacta gataaaaata ggaaagcagc
    54121 aacacaagaa ttaatagata aggtccaaga atatatatat ccattgaata cagaagaagg
    54181 tcaaaatcga gatggaaaag ctcctatagg tgctattgta actatagcta ctccacaaac
    54241 attactaatt aatgtaaaag ctagttttgt gttttcaaat ggatttagtc aagaaactgt
    54301 gctgaatatc ttaaaaaata aaatagacaa atacctcgat aaaatagata ttggaggtac
    54361 tgtttcttat aatgcaattt attcactaac aggttctatg atgcttgcag atgaaggaat
    54421 acaagatttt tctaacttga caataaatga tggaacaaca aatattattt taaatgacca
    54481 agtcgttgga ataggtgaga taatcaatga ggtgatagcg tgataacttc taaaaaaggt
    54541 agagaaatgc ttttaacatt atctcctatc tatgaacaat ctttagtcat gaactctata
    54601 tatgaagcta taggaagcga atttgataat ctagagttat tgaatgaaga aatagagtta
    54661 caattatttc ctcagagtgc gacatggggt ttgagctttt gggaaaatag agtaggttta
    54721 actaccaata tagatgaaga tatagaaatt agaagaagaa aagttattgc taagcttcaa
    54781 agtaaatata ttatgacacc taaaagaatg tctatgatac ttcaatctta tacaggtgca
    54841 aacataaaaa taaatgaaaa tatatctccg tatacttttg gtgttgaatt aactagtact
    54901 caaggttttc ctagagattt agaagattta tataagagag taaatgttat aaaaccttct
    54961 catttagctg taagttataa attagtatct ttattgaaaa gtaaaaccta ttttgcacaa
    55021 acagcaatta tgagtgaaga aataacagta tatccttata caagtaaaga agtaaaagca
    55081 agtgttaaag ctaagtttgc attagctaat aacataagtt cagaaacatt aacagtatat
    55141 ccaaggtagg aggtggtaca aatggctgat gaacaatttt acacaatact aacaaatata
    55201 ggcaaagcta agattgctaa cgcaggaatg ttaggtaagt cagtagtttt agagaagatt
    55261 caagcgggag atggtggagg aaattattat aatccaacag aagaccaaac agcattaaaa
    55321 aataaggttt gggaagggaa tataaacgct tttgataatg atgaaaataa tcctaactgg
    55381 attattgcaa cagcatgtat ccctggttcg ataggtggat ttacagttag agaaatgggt
    55441 cttatagata atgaaggaga tatgattgca gtttgtaaaa gcccagaaac ttacaagcca
    55501 aaagttgata atggagctat gaaagatttg tatttgaaat ttatcataga ggtatctaat
    55561 gtagagaaag tgactttagt tgttgaccca actgcaatat ttttgactaa aaaagatgaa
    55621 gaaaaaatat tatcaaatat taataagtta gacactaaac tagatacaac taagacagaa
    55681 ttaacaaaca gcatagaaac tgctaaaaca gagttaaaca acaaaatagg agatacaaca
    55741 caacttgaaa caacggataa aactagtctt gttggtgcat taaatgaggt aaaaactagt
    55801 gtagatagca tagaaacaac agcagagaaa acaagttata ataatgcaac aagcaatctt
    55861 actgctacga atgtgcaagg ggcgattgat gaagttgtta gaaagataga aaatttcaat
    55921 gaggttaata tatctataca aaatgatatg ttacctattt aagagaggag agtgagaaaa
    55981 tgcagactga atggaatttt gattttaagg cagaagcaca accaattact ctaaaagcag
    56041 gtaaatataa attagagtgt tggggagctc atggaaaggt ttggggtgga gattctcaat
    56101 ctagtggagg atactcttat ggggaactta cattaaaaaa agaaactaca ttatatgtat
    56161 atacaggtgc tactggttct agtaataaat atgaagaatt tacatttaat ggaggaggac
    56221 ttggtgttaa tagtggagga ggtggagcaa ctgatataag acttgtcaat ggagattgga
    56281 ataataaaca aggtcttttg tctaggataa ttgttgctgg tggtggtgga ggcgcattta
    56341 gtaaaactcc tgctggcaaa ggtggtggtt ttaaaggcgg aaatagtact aatgatgata
    56401 acagttcgat gcttattgta cctggtggta cacaatatga tggaggtaga ggttattgtg
    56461 atgaatggga tggtgttttt ggttgtggtg gaggttctat tcttggttta gaacaaggta
    56521 aataccctta taattcaggt ggaggtggat ggtttggtgg tgcaggtgct agaaatattt
    56581 caagtggtgg aggtggaagt ggctatgtat taactaaaga tagctataaa cctgttggat
    56641 atatacctac atctgaatat tggttagaaa atgttggtag tattactggg ggtaatactg
    56701 ctaaagtaaa tggttatgct aagataacat tactgcaagc attaccattt ttaacagtat
    56761 cttcttataa ttccacacaa gcaacattca aagctgccca cacagaccct acattattaa
    56821 ctaaaataga atattttata gatgatatat taaaagaaac tataacaacc gatttaactc
    56881 ttgaaaaaac aattaactat acattagaag ataatgcact gcacacactt aaaatagttg
    56941 ttacagacag cgctaatgct acagcagaaa agatattaag cataagtaaa ggaattgcac
    57001 ctttgccaag tgcttctagc ttagaagatg ttacgaataa atggatggaa attaaagatg
    57061 catttaaaac tggtaaaaca agtattataa acactttagc attaaaaagc atagaggcaa
    57121 acttgaataa tacactagtt gagctatcag agaaaataaa aacaagtttt gatagttcag
    57181 atgctagtgt acaagagtta gaaaatcaat tactaattgc agaaaatgag aaagtgggag
    57241 gaattttata atgaatataa ataatgttgt agtaagaata ttagcagaga gaattttaag
    57301 tggaggttta aaccctctaa aaaatcgaga atttgaactg gatgatgtaa ctaatgcaga
    57361 gtacagaaaa gcagtagagg attatattat agagcatagt ggagtagtag aaggagcaga
    57421 accaacaaaa taggttcttt ttttatttca attaattagg aggcttacat gaatgaggaa
    57481 gttataaaag aaaaaataaa acgaaatgaa ataagaataa acagacatag tgatgaaata
    57541 gacgaattaa agatagcaaa tatagagtct aaagcagagt taaaagcatt gtgtgagaac
    57601 ttaaactcac ttacaagtat gctaaagtgg ctaattggaa ctatgattac aaccttagta
    57661 gggttcttta tatttgccat acagaaagga atattttaat taattaggag gataaaagat
    57721 ggataattta ataagtttta taccagagca gttactaatt ttagtagctg ctctttatgt
    57781 tataggagca ggttgcaaga aatataagca attagataac aagtatattc cagtagtgtt
    57841 attagtactt gggataggat tctctatttg gatgttagga ttaaatccta gtgcagtctt
    57901 acaaggtgta atttgttggg gtatatcaat aggtataaat cagacttaca aacagttgag
    57961 ggaggagaat gaataatggt agatataata aagatgttaa caaagaaaaa gtgttatcca
    58021 aacaagaata gtcctaaatt catagtgatt catgagactg ataatgagga caagggagca
    58081 gatgctaaga gacatgcaca agcacttaat aatggtaatt tagaagcaag tgttcattac
    58141 tatgttgatg ataaagtaat atatcaaaca ttagaccata aagatggagc gtgggcagtt
    58201 ggtaaaagct atggaactgc attagttgca ggagttacaa attacaatag tattaatata
    58261 gaaatatgtg taaatcgtga tggtaattac acaaaagcaa gacaaaatgc aatagattta
    58321 actaaaaaac ttatgaaaga tttaaatatt agtgcagata aagtaattag acactatgat
    58381 gctaagagaa aatattgtcc tcgtaagatg cttgataatc cgtcattatg ggtagatttt
    58441 aaagataaaa taaaaaatgg tgggggtgtg aatagtatgg taaaatacac aatagtctat
    58501 gatggagagg ttgacagaat accagccgag gttatgggtt ggaattacaa gaaagaagaa
    58561 tgtagagtat gtgatataaa agattatgta ccaggtcaga cacaaaatct ttatattgta
    58621 ggtggaggag catgtaataa gataagttct atgactaaag aaaaatttac tatgataaaa
    58681 ggtaatgata gatttgacac tctttacaag gtattagatt ttattgatag atagattaga
    58741 aggtagcagc tagagttagt tgttacctcc tttttacacc gcaattaaaa caatttttat
    58801 aaaagatagt attttacaaa taagtaaaat tgataattca agttaaagat atttacttac
    58861 attttgccta cactttatat acaatttcta gactattttg taattcacat aatatatatt
    58921 aaatgtaata tatattatat gaaaataaca aggtatataa ttacatgtaa aaaatgtatt
    58981 aaattgataa tgattattat atactggtta attattaata caaagggtat tacaaatata
    59041 aaggatatca aaattgttca gaaaagagta tagtatgaga aaatattaaa ctttaatgaa
    59101 aaaagtaaca tgtttatata atca
//
LOCUS       DAEPXF010000004        57141 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain HB27
            SAMN13376389-rid12479423.denovo.004, whole genome shotgun sequence.
ACCESSION   DAEPXF010000004 DAEPXF010000000
VERSION     DAEPXF010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13376389
            Sequence Read Archive: SRR10518432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 57141)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 57141)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001069624.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001069624.1
            Genome Coverage       :: 315x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/15/2021 01:54:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,165
            CDSs (total)                      :: 4,076
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 89
            rRNAs                             :: 10, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 10 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 19 of 69
            Pseudo Genes (incomplete)         :: 44 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 10 of 69
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..57141
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13376389-rid12479423.denovo.004"
                     /strain="HB27"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="China: Hebei"
                     /collection_date="2017"
     gene            143..493
                     /locus_tag="KRL67_000235"
     CDS             143..493
                     /locus_tag="KRL67_000235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HBH1521144.1"
                     /translation="MSAKVTNQYGNIEIDNQVIAQVVYRAAMESYGLVGLAYKSKGIV
                     ELLKGENATKGVRVEEVEEDAIAIELYVIIQYGTNISTVANNIIDRVKYVVEKMTGVR
                     VTRIDINVQGIRVK"
     gene            512..2155
                     /locus_tag="KRL67_000236"
     CDS             512..2155
                     /locus_tag="KRL67_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HBH1521145.1"
                     /translation="MIQYIDGKRLREMFISGANNLQNNKDLVDKLNVFPVPDGDTGTN
                     MSLTISYALKELAKVENDNISDIGKALSKGSLMGARGNSGVILSQIIRGIAKSIEGKS
                     KLSTEDLAKAFKNGSDTAYKAVIKPIEGTILTVVRESGEFAIKTAKKEKDVVKFLSML
                     VKESNSSLERTPDLLKNLKEAGVVDSGGKGLVLIYEGMLASIKGDNIEIKNADLDTNI
                     STSMDFAKSTTSTDDIKYCYCTEFILESSKVEDTKIRDIMMAYGDSLAVVGDDGVIKV
                     HVHTNDPGNVLQEALKYGQLLTIKIENMKLQHENTLLDVEEKKENDSEPLEEEKEFGF
                     IATSMGEGLANIFKDFGVDHIIEGGQTMNPSTEDFMNAIKDINAKNIFIFPNNSNIIM
                     AANQAKELSDKNIIVIPTKNTPQGFAALVTFNGELSEDENKEAMMNALNSVKSGQVTF
                     AVRDTVMNEIDVKEGNIIGIAEGNLLSAGDYVDEVTSNLIEKLVDEDTAIITLFFGED
                     VTESQANELRTSLEEKFEDVDVELYYGGQPLYYYLISVE"
     gene            2236..4293
                     /gene="recG"
                     /locus_tag="KRL67_000237"
     CDS             2236..4293
                     /gene="recG"
                     /locus_tag="KRL67_000237"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HBH1521146.1"
                     /translation="MDLYKDVQYVKGIGPKKADKLNKLGIFTLKDLLYYFPRQFEDRN
                     NLKKIAQLEDGEKVTIKAVISSINTFSPKEGMTLTKIDVKDETGSAKLVFFNKSYIKN
                     TFRPGDSILVFGKVKKKFNNLELTSCELEYLTNSPKNTCRFMPVYQLTYGVTNKEIMS
                     IIRTVLEDKDLIIQEYMPQRIIEKYRLCSIDFAVRNIHSPSSKESLKIALYRIVFEEL
                     LILQLGLFVFKSGRNKEDGIKFETSKDLKKIISALPFKLTKAQNRALDEIIQDMNLEK
                     IMNRLVQGDVGSGKTVVALLALANCVLNGYQGALMAPTEILAGQHYISLTESLKDFGI
                     NVGLLIGSLTKKQKDTVLEQIKNNEIDILIGTHALIEDKVEFNNIGLVITDEQHRFGV
                     MQRSKLSLKGANPDILVMTATPIPRTLALILYGDLDISIIDELPPGRQPIETIAIEKS
                     KRDRAYNNLVRREVESGRQVYIVCPLVEESEAIEAKSAVELVEELRAEYFHDLRLGLL
                     HGKMKSSEKDEVMGLFKNKEIDILVSTTVIEVGVNVPNATLMIIENAERFGLAQLHQL
                     RGRVGRGSHKSYCVLIYDSKTDVCRQRMAIMEETNDGFKISEKDLEIRGPGEFFGTRQ
                     HGLPELKVANLFKHIKILKLAQQEARYILGEDNNLQLKENMALKKEIIDKFKDTLKEI
                     SLN"
     gene            4352..4906
                     /gene="rsmD"
                     /locus_tag="KRL67_000238"
     CDS             4352..4906
                     /gene="rsmD"
                     /locus_tag="KRL67_000238"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HBH1521147.1"
                     /translation="MRVISGKARGLKLNTPKNEDVRPTTDRVKESLFNMINSYIMESE
                     VLDLFAGTGSLGIECLSRGAKSCTFVDISKESIDIVKSNIKKARVESESIILNLDFKT
                     AIDKLKLQNSKFDIIFMDPPYYKNMFIEAIEKIDNSNLLNEDGIIVVEHDTNDLFPDK
                     ICKLEKTRDKKYGNTTLTFYKMEA"
     gene            4908..5405
                     /gene="coaD"
                     /locus_tag="KRL67_000239"
     CDS             4908..5405
                     /gene="coaD"
                     /locus_tag="KRL67_000239"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HBH1521148.1"
                     /translation="MENKPRKAIFAGSFDPITNGHLDIICRASKLFDELQIGVLNNPN
                     KKGLFSFDERVKLIEKSTSHLNNIKVVTFDGLLINYCQENGIGALVRGVRSGADVDYE
                     LQMAHMNRELNPDIETIILPSCTKYSFISSSLIKEVLLFDADIKNLVPKIVLEELKKK
                     TSGGN"
     gene            5409..5933
                     /locus_tag="KRL67_000240"
     CDS             5409..5933
                     /locus_tag="KRL67_000240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH1521149.1"
                     /translation="MKIDLEMIELFEQLEDIFRSASTIPFSHKCTVDKDEVLAIINDI
                     RTLIPEEVTQAVWINKERNKIISQAKQDATNIVEQAKKEAERIQQEYQDNIEELKKNS
                     EDVVKAYVESSEPVVQADQRAKDIVDRAERIANEIRIGSIEYAEDVLSSVEYNLREIL
                     EEVKRDKAELRPKK"
     gene            6277..8196
                     /locus_tag="KRL67_000241"
     CDS             6277..8196
                     /locus_tag="KRL67_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HBH1521150.1"
                     /translation="MKERAKKILDFIIKNNNVTSQELQEKFNVSKRTIYYDILAINKQ
                     LGKSGNIKNVKHKFIFEGNLCDARKIISAEEDKFLDSDYRKTFILNKILLGEKISIEK
                     LTNEMLLSKNTVVQTITDVKKYLQTMGLRLEYKGKYKIIGDEYVIRELFLIIVQENVL
                     EINSISKEVSSFDTKGHIKLTDYSLLNLTKFVEFLNKRIRDGKTLYSYKYLNEAKKIS
                     YFSNCKELLCEEANENEQAYICTYISSLPSLNSEVKEDVVEEYVDKLIDKFEVNTAIK
                     LESKHEFKKNILRHLHSSYNRIRFKFPIRNPMLDETKYKHESLYKIIKSIIENEEEFP
                     VFEGIREEEIGFIAAYFGGYLRGSRDNGLRRNKVLLVCPNGLMVSKSLEIQLYKYIPT
                     IEIVGIVSIKQLKEVNVYYDYIITTIDIQNVNNVIVVNPLLTSSDVQLLMNKLISVKE
                     NEKYFNLELIIQAIRKNGVINNEEALKVDLLNIIHKIDEGEMYQPMLKELINAERVNI
                     IKSVRDWKEAIKIASKPLLEDNSIEELYIENMIKSVEKYGPYIVLADRFALPHASSKE
                     GVNKLAMSLLIVEDEVDLLGKPVNIFMVLAAVDNTTHIRALASLSEMMYEEENVKLII
                     NGDKSSILELINKQN"
     gene            8209..8496
                     /locus_tag="KRL67_000242"
     CDS             8209..8496
                     /locus_tag="KRL67_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBH1521151.1"
                     /translation="MKILAVCGFGVGSSMVLKMTIQKALKQLGIECEVENTDINSARG
                     ADCDCIFTSQELLNELKGTCNVEVYPVKKYMDLAEVTEAVKTFLSDKNDKK"
     gene            8629..9888
                     /locus_tag="KRL67_000243"
     CDS             8629..9888
                     /locus_tag="KRL67_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HBH1521152.1"
                     /translation="MLDFISNNILRNPSMLIGLIAAFGLVLQKKKASDVIKGSLLAAF
                     GIIIMETGTGMLVSSIAPINGAFQSISGGAVTKGLSDVTFTASYGGIIGLAMFVGLAI
                     HLMIARFTPIKTIFLTGHMLYWFPFIFVASGVEAGLNGGVLIAFAAILSALYWSIMPW
                     LLRKYVFDVIGDDSFTLGHPSGCLALISGFVAKLVGNKEKSTEDINIPQSLSFFREIS
                     ISGGMAVFLMFIVVGIVIPGAFKDSNEFIGTYAIKQGLSFGAGLIIMLHGVRMLINQI
                     MPAFQGISEKVVPNALPAFDCPILFNYKPNAVLIGFLVAMLTSTAMILICNYFNVFNL
                     LLIPLVITSFFECGAAAVIGESQGGIRGAIIGTAIAAIFMVVLVGISAVIYSGTIQNW
                     ILIFGGNDLSLFGIISKFIGSLFGGIL"
     gene            9891..10631
                     /locus_tag="KRL67_000244"
     CDS             9891..10631
                     /locus_tag="KRL67_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HBH1521153.1"
                     /translation="MKFKYIDEIRRLIDVIENEEKVNMEKAVQILVEAIESKNAIFSF
                     GASHAGILTEELFYRAGGCVIINPVFAKSLMLDTAPITHTSKMERLEGYGTALADKTP
                     IKKGDVVIVHSVSGRNPVSIEFAMKAKENKAIVICITNLSYSKSVSSRHSSGKKLYEV
                     CDLVIDNHGQKGDACVEIDGLNQKVSPTSTVIATTVMNSIIAQATQELVNRGLKNPPI
                     FYSANIDGGDELNKKIFDEYKDVIHYEY"
     gene            10954..12264
                     /locus_tag="KRL67_000245"
     CDS             10954..12264
                     /locus_tag="KRL67_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HBH1521154.1"
                     /translation="MKKENKMEGIKVGMLKKLCSLAMVAIMTTTLITGCSSGNGKDSK
                     KDGEKEKLVLWMPPVENNMKEVWDPILDKFEEKNNCEVDLQIIPWDNYSEKFATAISA
                     GEGPDVGYMYAEMYPQFIESGAVEDLTNYATKEDKEQSIYIKTSEMMGGMYGMPFQAA
                     NPGVLYYNKDILEKLGEKPPKTWEDFKRICKKATQDTDGDGKIDQWGLAQGWGAKTFG
                     NLNWNWYPYLWQAGGDIFNDDLKSVKFNDKSGLEAANFLKELQAYVPEDSLSKDSNEM
                     IESVFGPGKAAFTIMLSSAATSVFDESFPDLNWGFVTGLEDKKAATFGAVDHLSLMSS
                     AKNKELAYKLIQHMLSVESMTEFHKAIPRAPITKGEPYQGDERFKEMVENDKDVYRPL
                     VVGPHGVEIYEYLWKELQTMISGDKTPKQALDDAAKYSNDLLAQ"
     gene            12362..13279
                     /locus_tag="KRL67_000246"
     CDS             12362..13279
                     /locus_tag="KRL67_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HBH1521155.1"
                     /translation="MENLVREFEENSRRKKTKLNIKPYMYILPLGIILVAFYVIPIIM
                     SIYFSFTKYNIISPATFIGLENYKKLFTDETLKVSIINTIKFTVVVVPCQTILSLILA
                     VWITGKGNSKIASFAKGAIFIPVLSSMVLIGMVWRALLNGEGSIIYQVLGAFGIESSK
                     LLGDSKTALPTLMFISMWKNIGYFMVIYISAIMNLPKHCYEVAKVDGATKLQEFIKIT
                     VPLLKPTTIMVVFLGSIWSLQVFDLVYTVTGGGPGISTMSIVMHAFNLNFKNFNSGYA
                     MTVANVLFLLIAVVSILQNKLVKRDNSDF"
     gene            13295..14104
                     /locus_tag="KRL67_000247"
     CDS             13295..14104
                     /locus_tag="KRL67_000247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HBH1521156.1"
                     /translation="MKTARRLLSDGLLLFIACASLVPFIYMLIISLKITYNSYSLDIS
                     FSTVTLQNYIDIFTKKGFAQYFLNTAIVSFSGVLLNLVFSTLAGYSFAKMDFKGSDKL
                     FLFMIMTLIIPSQVTMIPLYIIMKHLGWINSYLALIMPIPTAFGVFIMRQAILGVPKE
                     LLESAKIDGCSDFRILIQIVLPLIKPALITLAIFTFMGAWNEFMWPLIATTKDAMRTL
                     TVGLSTLKTFQITNYGQMMAGATITFLPPFIFYLILQSKFVEGVSLSGIKG"
     gene            14162..15910
                     /locus_tag="KRL67_000248"
     CDS             14162..15910
                     /locus_tag="KRL67_000248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4091 domain-containing protein"
                     /protein_id="HBH1521157.1"
                     /translation="MEISYVLKDCTFKHNKYSVIEAETWKNKDLGIVVAKGEKFAFQI
                     MLKATEEFNCTIDKSSTISWKGLGNRVRLALNVPNSLENNFSINILGYVQDDTKAFVN
                     DAILRDKDVLVEQYLPQTFWIEGQVPEDFEENNLDIGIDIFKSFGYEDEEKVCTIDVP
                     VKVRNVVLKPLDESKFFLDLWQHPSCLARMYKVELWSDIHFEIVDNYLKELASLGEKV
                     ATVIVSDYPWAGQSCYKVYKNPSNLYEYNMVSVSKGLDGKIKCNFESMDRYISIADKY
                     KMAQEIDLFGLLGNWCAGEFGNPVEGYKDPIRVRYFDENDKVFKFINNTNDLKEYIGL
                     LLNHLIECGLWDRVRIIADEPNNPEVVKECIEFINSTVGTHQVKYKSATHDQNFLDRA
                     KDEIDDMSINLKLTIQNYKDIESLKKKINDKGGILTWFVCCFPEKPNSFLSSPFVENR
                     IIGWYTYYFGLDGFLRWDYNLWTEDPWKDSSYKFPIWKAGDMFFVYPGKDLKPVRSVR
                     MENLRFGIQDFELFTMLEKEKGREYIEVELMQELLNKKENAEIKGFGDIELGYSLDNC
                     GYDKVKKYVLELLDRV"
     gene            16172..17194
                     /locus_tag="KRL67_000249"
     CDS             16172..17194
                     /locus_tag="KRL67_000249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 5 protein"
                     /protein_id="HBH1521158.1"
                     /translation="MFGGIKIKRIGIIIMFFLSIAFIGFYLVIRDNSDTVKMQRGINI
                     GNALESPKDFPWDVKMSNKFFDDIKDAGFDTVRIPVRFSDYTSDFDNFKIDEEFFKKI
                     DKYVDYALDKDLIVVLDLHHFEEIMKEPRVHKEEFLKIWQQIANRYQKYDKKLVFELL
                     NEPKENLSSQLLNEYIEEAIKIIRKTNPKRTIIVGPYNFYQIDYLNELNIPKDSNIVV
                     SFHYYEPNDFAFQGNIYHKGFEHLSNITWEGTNEQMEYLKKRFDTVENWANKNKVKIF
                     LGEFGITKEAPEASRRAWIKAVREEAEKRNFSWAYWELASGFGIYNQIEGTWDRDILS
                     ALIEKR"
     gene            17199..19505
                     /locus_tag="KRL67_000250"
     CDS             17199..19505
                     /locus_tag="KRL67_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HBH1521159.1"
                     /translation="MKKNSKKILITLTIITNIIYILWRIFYTVPKEEGMFALICAIIL
                     LFVEIMGMMEMFVHYYGMSNIEYPEKPIISEELYPHVDVFIATYNESVDLVRKTVNGC
                     IHMQYPDKKKVHIYICDDGNREEMRILAEKMGVNYITRTEREGAKAGNLNNAMQHTNS
                     PLIATFDADMIPMHDFLIATVPYFLKNEQAKKDGEKEEYEKVGFVQTPQSFYNPDLFQ
                     FNLHSERRIPNEQDYFYRDIQLARNRTNSVIYGGSNTVISREALEEVDGFYTYSITED
                     FATGILIQSKGYRCYAIPEVHASGLSPTDLKSLIKQRERWARGCIQTGRRLNILFRRG
                     LGFWQKISYISSITYWYASIKRFVYIMAPILFSVFNVIVVKCTLLQVLVFWLPMYILS
                     SLSLKIFSQNIRNIRWTNIYETIMFQSLMPAVILETFAISKNKFSVTNKSKLEENRMY
                     KFLQGIPYFIYMVLSIIGILKMFVAIFKMSSITYSVVLFWLIGNLFNLVMATLFISGR
                     QQLRKSERYIAEIDFKLKQNSYVLSSKTIDISENGFAFLLENPEYISPEEEFEVEFRE
                     KSGNEMYIANMKAKIVNVVEVNSKWKYAAYITHIEDSEIDNWMCIVHDRIPTLPMTIS
                     NQLGFFDDLQINVKKRIEKTRTLSRRSPRINMNFQMDIKNTGKLRIVNFNYQYVLLNF
                     ENKNIYPKEIELEINEGIVLECDLCEGKIDERGILYRVNNIDSIMQNFFLRDEMMDWI
                     LQNKTIFVSKPSEKKEKSIDEFEPMEYI"
     gene            19477..20592
                     /locus_tag="KRL67_000251"
     CDS             19477..20592
                     /locus_tag="KRL67_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH1521160.1"
                     /translation="MSLNPWNIFRRDEMKTLKVFFCSAKYLILVFIFFINTTFISSAT
                     SDKILDLSFKKIETDLSSKITYEDTGVKIETDSSKSDKERYLYIYQNIKENWSMYNNF
                     YIEIQNKNKSSQRINLSVQSKNMLEFRLKEGSEIFLEDKNIVYSDKIKEGCIEVPGEF
                     EGKIYVNFNSLINEESNVVLDSNMLSNIVSWGITFIPSDEEHNIVIIKKISLLSKEKL
                     VFLNNIKIIGDEEVQIPVLGQSISQYEVLGLKSDSKIKYSLMGKQDNVSISQNGKLTL
                     SNKAKPGKIILQVNVDDKFKIGKKITLTESWSVNKKDKDGVPYTLVSPEQSPTVQDMK
                     KINFMNNIITFVRILFVSLVIICFGIYLYWKKCSKTK"
     gene            20610..22028
                     /locus_tag="KRL67_000252"
     CDS             20610..22028
                     /locus_tag="KRL67_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBOAT family protein"
                     /protein_id="HBH1521161.1"
                     /translation="MLFSSLIFLFYFLPITLVLYYVFRFNRTIQNMILLAVSLFFYAW
                     GEPKFVVIMIVSIIMNYIFGLLVDRYRESKIKVKIFLVLMCVYNIGVLFIFKYLAFAL
                     RNISELVNIELTIPNIILPIGISFFTFQGMSYVIDVYRRHGEVQKNPFYVGLYIAFFP
                     QLIAGPIVRYESVAEQILNRKETWNKFSIGTCRFITGLGKKVLISNNMAIVADYIYTM
                     NSQGEIAASLAWLGSIAYTMQIFFDFSAYSDMAIGLGLMFGFKFEENFNYPYISKSIS
                     EFWRRWHISLGMWFKSYIYFPLGGSRVANKDIMIRNMFIVWLFTGIWHGAEWTFVIWG
                     ILNFVFLIIERFIMFEKIENHNFIKHIYALLVVNFGWVLFRSPNLKEAYNYFKAMLGG
                     NGVIWSDYTYMFLKEYWVFFIFAFIFSVPIAKKINKFVVEQVKYSMIFNVFYPISAIL
                     LFFISVTYLVTGSYNPFIYFNF"
     gene            22044..23252
                     /locus_tag="KRL67_000253"
     CDS             22044..23252
                     /locus_tag="KRL67_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH1521162.1"
                     /translation="MNRFFKVRLITAIMFLIVLFAFSIFNICYSGKDMIQEIKNLVAE
                     KSSLKDTISSIDNTVNDKVIYKNTFIETYGYLQKLMGKNEFSKFSSVKDTSGKMHYTY
                     FAKAPNPIGVISDRVRKFSEEMEKQNTKLVYLMTPDKYIQGVTKFPKGIPYSYSNETA
                     DNFLNQLKKYNVDYIDFRENILKSGIPKDDLFFKTDHHWKVETSFWAFGELVEQLNKK
                     YNMNLDENHYYRDKENYNSILYPKSFLGSMGRKSGILYGGIDDFTLIYPKFKTNYTYY
                     TDSKFQKFELKGRFEESLVLSYPFNADLNLMDGQSDKYSTYLLGNRPLVKIKNIENPD
                     GLKVLFVKDSLIVPTAAFFSSVCSSIDMIDPRYYDGDILEYAKSHDYDFVFISIYPQN
                     LTKEFFPFCE"
     gene            complement(23333..24721)
                     /locus_tag="KRL67_000254"
     CDS             complement(23333..24721)
                     /locus_tag="KRL67_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:dicarboxylase symporter family
                     transporter"
                     /protein_id="HBH1521163.1"
                     /translation="MESSFLSQFLMITDFKTIFFIILLLGTFFIVRQFEKKKIKFSLR
                     TIYATVLGLILGVIIQAFAGFPNDTTQVTWLQEVSKWYSLFGSGFMDLLKMLVVPLVF
                     LSIIKVIINMKDNNLGTLTFKSLGMLLATTAIAAIVGIIVANIMKLGVGANMPTLVEN
                     QELKEINSLVDTLRGLLPSNPVMAMANGNIVATIIFATFIATSIKRLSKKHFETIKPF
                     IDFIEASYKIIVSVSMTVIKFMPYAVVALLANTITSQGISSIVSVLQFILALYISVAI
                     LFIIHLIFITLNGLNPITYIKNSTEALLLAFTSRSSLGTLPVTIESLVQNHGVDEGVS
                     SFTASLGANMGMNGCAGIYPALMAVTLANMAGVNMDMSFYVMLLIVITISSFGIAGLP
                     GTATMAVSVVISGVGLGSYFPLVGGILAIDPILDMGRTMINVNGAMVSSITVGKSLGK
                     IDKEVFSKKNIS"
     gene            25063..26397
                     /locus_tag="KRL67_000255"
     CDS             25063..26397
                     /locus_tag="KRL67_000255"
                     /EC_number="1.8.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-disulfide reductase"
                     /protein_id="HBH1521164.1"
                     /translation="MRVVIIGGVAAGMSAAAKLKRIKPEYEVVVYEKTEIVSFGACGL
                     PYFVGGFFDDADELLARTPEKLREAGIDLNIFREVVEVDSESKKIKVKNIKTDEIYED
                     YYDKLMIATGARSIMPPIKNIKLKNVSTLKSLYDGEYLKKLLSNEDNKRVTIIGAGFI
                     GLEAVEACKKLGKDVDVIQLEDRILPQVFDKEITDVLEEEINRHNVNLHLDEMVVELY
                     GEDKVEKVITNKGEIDTDVVIIATGVRPNTEFLSNTNIKMLKNGAIVVDEYGRTSVED
                     IYSAGDCATIKNIVSNENVYVPLATGANKLGRIVGENLAGREVSYQGSLSSSCIKIMD
                     MEAASTGITERQAKDLGINIKSKFISDYNQTHYYPGRNKIYVKLIYDADTKVILGGQV
                     AGFKDAVQRANVLAAAIFGKMTTSQLGMLDLCYAPPFARTWDVLNVAGNVSK"
     gene            complement(26526..27443)
                     /locus_tag="KRL67_000256"
     CDS             complement(26526..27443)
                     /locus_tag="KRL67_000256"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011969064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HBH1521165.1"
                     /translation="MIDLTLLGCGGNVPMPNRFLSSVFINYKGRKILIDCGEGTQVSM
                     KLKKCGFKDIDLICITHLHGDHIFGLLGLLSTIGNSGRTSDLTIVGPVGIVDCVRSMR
                     NLVEYVPYTLKIIENPQGNFSLDNKVLRNLEISTISLEHSIECLGYSFNFRRNPKFDI
                     DKATKNEVPKILWNKLQEGQNIVLDSKQYTPDMVLGELRKGVKISLTTDTRPIESIPD
                     FIKDSDLFICESMYGDDLDISKAVRNKHMTFREAANLAKLGNVKQLLLTHFSPSLDIP
                     SMYLENATNVFENTILGEDRLSLRLNFDE"
     gene            27651..28535
                     /locus_tag="KRL67_000257"
     CDS             27651..28535
                     /locus_tag="KRL67_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4846 domain-containing protein"
                     /protein_id="HBH1521166.1"
                     /translation="MKVLKKITILSLITLTLVGCSNGEEKKVVEGSKKTQNSKKDEQP
                     IIVHKDANILKDRYEPPKGFKRVDVEKGSFESFLRNTKLKNYGEKVKYYDGRTKESKG
                     IYDSVFDVDIGNRDLHQCADAIMLMRAEYLYQNKLYDEISFDFVDGFKAKYSKWAQGY
                     RISVKDSGSSWYKATEYSTSYESFRKFMDIVFAYSGTLSLEKELEPVKVEDMKIGDVF
                     IVGGSPGHASIIMDMAENEKTGEKVFMIAQSYMPAQQTQLLINRKNPNLSPWYSLDFE
                     GDLTIPQWTFKRDQLKRF"
     gene            28748..30589
                     /locus_tag="KRL67_000258"
     CDS             28748..30589
                     /locus_tag="KRL67_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional metallophosphatase/5'-nucleotidase"
                     /protein_id="HBH1521167.1"
                     /translation="MKIRRYLILVIIILITTSQSFISYGLDRFEEITIFHTNDVHGRY
                     AEGDDHIQMGNLATLKKETPNSILVDAGDCLHGLPIVNMDRGKSAIELIKCAGYDYIT
                     PGNHDFNYGKDRLFELSKSANLGENTLKFLSSNIFENGKKVFDSNDIKEIDGIKIGFF
                     GLSTQETKSKTGFKNVEGLEFKNPIESATEQVSELKGKGANVIVAICHIGTNSLSKPN
                     SIDIANEVQGIDLIIDGHSHTKFENGKQVGDTLIVSAGQYLENIGEIKLTLDTNDVEK
                     IKIENKSARLIGKEEALKYKLDSVVSKKVDEIKEQQEKILDKVIGVTTNTLDGSYENV
                     RTKETNLGNLISDILLDKTKADISLFNGGNIRDTIEKGNITRRDIVDVFPFSNTIVTK
                     ELTGAQIKNVLEHGVKLYPEKSSAFLQVGGISYYFNPKQKEGERITSIQKEGKPLDLN
                     KKYVVATNDYIASGGDEFPCFSKEPVLKEFGNLESTVIEYIEYKKEISKNVDGRIGIK
                     SKDELIRKNHESDLILSENEKNINDNEENLSNNIKSSDSDKNKSLDKTSARESSNLGI
                     SKREAKEKSPKTGDLGFSNSIIIFLVSSTLICLLNFNQKELKDKKSK"
     gene            30894..31601
                     /locus_tag="KRL67_000259"
     CDS             30894..31601
                     /locus_tag="KRL67_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBH1521168.1"
                     /translation="MNNILLLEDDKSLNRGISFKLKKEGYNVFSAFGIEEAKNIFIKE
                     EICLIISDIGLPDGSGFDFCEEIRKKSDVYIIMLTALDEEVDIVTGYDLGADDYITKP
                     FSLMVLISKVNALMKRVNTVKNYTLLVCDDLFFHYIENKLIIKTDDKEEEIILSKTET
                     KLLKYLMENSMQTLTKEQLLESLWDNSGNFVDDNTIAVNIRRLRQKVEKNPSEPKYIK
                     TVRGVGYMWGERSIKKC"
     gene            31595..32905
                     /locus_tag="KRL67_000260"
     CDS             31595..32905
                     /locus_tag="KRL67_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBH1521169.1"
                     /translation="MLKNMNLKDGLSVKDDYIMRRVIGYSILSVLIVTLVFILFEYSR
                     LHKLYEYQIKNTQNIAGILIEKYPNDEIDIVKSIYNSDYSKVSIGEKAFKKFGYGLEN
                     KMSNDKNFSLYLKSFFKESFFIFLMMFIVVIDIIYYFIRYINKRLSKIYFIVDKMAQE
                     NYLKDTTESEETSYLVEYNEYFKEGTFSKINNCLYELNRSLKIKFIKLEKEKESVKSL
                     VTDISHQLKTPLASLKLYNTLLIEEELEEDEANEFLLTNKASINKLENLINSLVNISR
                     LEISMINIKKEDNDIKNTILNAVKSVTPKAKSKSITLNLNEFNSRIIPHDKKWTEESI
                     FNVLDNAVKYTQINGEINISVEETANYFKIIIEDNGIGIEKNEFNNIFKRFYRGSADE
                     IESVEGSGVGLYLSRRILEEQGGNIIVSSKVGIGSKFSLFLTTM"
     gene            33022..33705
                     /locus_tag="KRL67_000261"
     CDS             33022..33705
                     /locus_tag="KRL67_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBH1521170.1"
                     /translation="MKILYTENLSKHYGKGESLVKALDNVNLEINEGEFVAIIGKSGS
                     GKSTLLHMIGGLDIPTSGKVYIDNKNIFTLKEEELAVFRRRKIGFIFQSYNLIPSLNV
                     WENVVLPIGLDGREVDESFIKELLKSLGLENKHDVLPNTLSGGQQQRVAIARALATRP
                     AIILADEPTGNLDSKTSDEVMSILKSMSKKYSQTLVMITHDDSIAQMADRVIFIEDGR
                     VSKVGDKND"
     gene            33698..36085
                     /locus_tag="KRL67_000262"
     CDS             33698..36085
                     /locus_tag="KRL67_000262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HBH1521171.1"
                     /translation="MINTTKIAKSNLKQNKSKSILIIITIILSTLMLSSIGIYIVDAG
                     AYQKENTIKYSGNYQGILANVDEKQADILSNHADVELTGKMNGVGVEKLEDDSNISLA
                     YMNKDALKLNSFEFVKGKLPIRENEIVLDSGALKALGYKNKDLGEKIKISYNDYKNDK
                     KIEKEFIISGILKTSEISEAGKYYYAIISETYMRNTRDMSQEDFNIYVKFNDKSNLSI
                     EQVKEKLNKIANDIGLDTINTAVNENYINALKPDMETIMGGVFVGLVIVLSSILVIYN
                     IFYISIVTKVQEFGKLRAIGATKKQIKNIVFKEGFILAGIAIPIGIILGYVLANIIIK
                     LFMDIDVKSSRLPVILSVVVISFISVVLSLLKPMKVASKVSIVDAVRYTGNKISNKNK
                     RKGYKNINLKRLSHANLERNKKRTYMTLASLILSGTIFITVSTALESFDAEKMAREHF
                     PYDIEVRLSGYEMNSDKNPKNNLNILQMDNPLSKDFFNQIKNMEGIKRIESARSVKIG
                     MEDYDVEFKYDLLQSINENDVKSLNKNLIDGKINLERLQTGDEIVITHVDTAKEMGVK
                     AGDKIRLTLYDGDKKIKKEFKVQAIAMGVPSFGIGKDFIDRTLKYDSTSSLGIYTEEG
                     KYQEIKDSIKKIAKNNGFLETDFIDSRIESNKATISFIKIMGYTLTGIIGVIGFMNLV
                     NTMITSIVTRKKELGMLQAIGLTNKQLVKMLNSEAVYYTSGMMIGSILFGGILGYIAV
                     ILLKKTGLSYATYSLPIVPILLMIVCILIAQFITTYLIGRSFNKESLIDRVRYSE"
     gene            36365..37507
                     /locus_tag="KRL67_000263"
     CDS             36365..37507
                     /locus_tag="KRL67_000263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--glyoxylate aminotransferase family
                     protein"
                     /protein_id="HBH1521172.1"
                     /translation="MNKKINYAPGPTETRENVRLARAEKTTNPDIDIDFVEFYKKTCE
                     KFGNIIGTKNDVYILSGEGILGLEAACASLTEKGDRVLVIDNGIYGEGFKDFVTMYGG
                     EYVLFSSEYTKSIDIDELKAFLDKDSNFKYATVVHCDTPTGVLNDVSKICPLLKEYGI
                     LTVVDSVAGMVGERLSVDESKIDIILGGSQKAISAPAGLTIVGISQDAKNCIKNRKTD
                     VIGFYCNLNIWEGYYEKKYFPYTMPISDIMGLDKALDNILEEGIENVLNRHEKIAYSV
                     RKSIEEYGLELFLEEGYSNTVTAIKVPESIGALKLTDYMLKNYNTLVATSLNQYMDTI
                     LRIGHMGENANLNKIEHILNVLDKSLSALGFKGNGNLLNLFNKYYF"
     gene            37530..38054
                     /locus_tag="KRL67_000264"
     CDS             37530..38054
                     /locus_tag="KRL67_000264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HBH1521173.1"
                     /translation="MEVTAKQKFTTKDMVETSLLIALVFIATKFINIRLPISINGGLV
                     HLGTAMLFISAIVFGSKKGALSGAIGMSLFDLISGWTLWAPFTFIVRGVMGYLLGKIV
                     WANGNNGNNFLINVIGICVSSIWMLFGYYVTEVILYGNFVVPLTSIPGNLMQVLIGLI
                     IALPISKVLRKCIK"
     gene            38154..38972
                     /locus_tag="KRL67_000265"
     CDS             38154..38972
                     /locus_tag="KRL67_000265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HBH1521174.1"
                     /translation="MLSVTRYSIKNIRLKPWVKFIWYFETKSNILLNNKLLPTDSIDI
                     ILNLSDVMEYKIENQDYTASNMHFNGIRDKHGFIIQYGNIRVIGISFYPFGLYPFLKI
                     PISEFNRQIVDLEAISKLFAKKLEESLNPTKAIEKVVLCLEEVLLSILEEDLISNKSV
                     ELLNSFIYVNRYSNIKAFCDDTNINIKTLERTCLKYTGYTPKILKRIYRFKMASNQLI
                     YNYKDDELFDFIYENEYYDQAHFIKEFKKFSGTSPIKFIGENKTIKENIKYSYL"
     gene            39050..39361
                     /locus_tag="KRL67_000266"
     CDS             39050..39361
                     /locus_tag="KRL67_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HBH1521175.1"
                     /translation="MKIITEIVEFNVEENLSKEEFIEIVNDLELKFHSIQSGFIDTEL
                     LYDEKSQKWIMIQHWESIEDLKSASSKMFKDSSTEKFRKALKNQTVKMNIIPQIKSWK
                     L"
     gene            complement(39491..40174)
                     /locus_tag="KRL67_000267"
     CDS             complement(39491..40174)
                     /locus_tag="KRL67_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH1521176.1"
                     /translation="MQKEAIMNKLIKDCVNFFNDCGFSYSICGGYALELFANMHIRPH
                     SDIDIWISDTDKEKSIQYMLNQGWDIYEPLDNYLLRPILDLNKQHIEQICAFAIKPDC
                     SFIELKKVEDGNYKMNIQCDEQLQFDFIELIYNPQKDGKFLFSQNQRIIRDMDKARLI
                     TSYNIPYMAPEVVLFCKSIYIDRVGYQKDFDVTVPLLSQEQRDWLVKALITAYPDGHA
                     WIDQLANQY"
     gene            complement(40266..40907)
                     /locus_tag="KRL67_000268"
     CDS             complement(40266..40907)
                     /locus_tag="KRL67_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBH1521177.1"
                     /translation="MGRKKEPDNTIQKILDISHKLFLEKGYEQTTIQDIVDNLDGLSR
                     GAIYHHFKSKDEIIKAITSRLYNDGFYGYDVKNDEGKTALEKLQYRLLHAYEQHNKQD
                     IFLMSSSLLRNPKYLATQFDEIINDIVPWIKALIEEGNQDNSMQVKNPEIAAQMFGFL
                     LNTWCIPTVYPGNPQQMIERFTLFGEQAAFMGISFMNDKIMCEYQKFVNKFSK"
     gene            complement(41081..43084)
                     /locus_tag="KRL67_000269"
     CDS             complement(41081..43084)
                     /locus_tag="KRL67_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha amylase C-terminal domain-containing
                     protein"
                     /protein_id="HBH1521178.1"
                     /translation="MKSQKRFKILDIDVYLQPFESDIKKRMKHYEDMKSKTLKNYKDF
                     SSFANAHLYYGFHQTNDGWFYREWAPNADSLSLIGDFNNWNRKSHLLRKIGSGNWEIF
                     IPGQNSLPHKSEVKVQVTANGKTFDRIPLYIKRVIQKDFGGFNGQIWQPKTPFIWTDN
                     DFDLKNITSPLIYECHIGMSTESESIGTYNEFTEKILSKIKKAGYNTIQLMAIMEHPY
                     YASFGYQVSNFYAISSRFGTPEDLKNLINTAHSMGIAVLLDLVHSHAVKNTLEGINEF
                     DGSEHQFFHSGSKGNHPAWGTKLFNYGKPEVIHFLLSNIKFWLNEYHFDGFRFDGVTS
                     MLYRNHGLGVSFDSYEKYFSMNTDIEAITYLQFANELIKEINPNSISIAEDMSGMPGM
                     CIPIKDGGIGFDYRLAMGVPDFWIKTISNLSDEDWDLGKMWYELTTRRPGEKNIGYCE
                     SHDQALVGDKTIIFWLADKEMYWNMEINSNNHVINRAISLIKLIKLITFSLAGEGYLN
                     FMGNEFGHPEWIDFPREGNNWSYKYARRQWSLSEDNNLKYKQLLNFDKSMLELTKYSD
                     IFIQNSHELIHMDNDKKILVYSKGKYLFIFNFHPNLIQSVDISKFQNTNYKTILNTDM
                     VEFGGNTKKENLRDYDVYFSSTPPSSKMPIASRTAIVLLDNGI"
     gene            complement(43237..43875)
                     /gene="recX"
                     /locus_tag="KRL67_000270"
     CDS             complement(43237..43875)
                     /gene="recX"
                     /locus_tag="KRL67_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HBH1521179.1"
                     /translation="MGIITKIEQQKRNDDRVNIYVDDKFFIAIFKELVYTFNLKKGNE
                     INEEELKPILDDEMYIKAKNKALNILSHADQSEKKLKEKLSSEFDENIVERVIDFLKS
                     YNLVNDSVLAQKIVNTNVNLNKCGKNRIKQNLYNKGINRSTIDEAVSELDKDTEFENA
                     MYLAKKRYERVKKEDKKKIYQKISQHLSYKGFDYDIIKRVLNKLLNFDEYDI"
     gene            44151..44840
                     /locus_tag="KRL67_000271"
     CDS             44151..44840
                     /locus_tag="KRL67_000271"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HBH1521180.1"
                     /translation="MRSENLVKMAELKNTEKLEKFNRHKGKIKIGILGGTFDPIHYAH
                     LATAEFIRDKYDIDKIIFIPSGNPPHKLCITTDKYDRYNMTLLATESNEDFLVSKVEI
                     ERKKRTYTIDTLKYLKKKYKNADIYFITGADAICSVEEWKDVKKNFELATFIAATRPG
                     ISLLRSQETIEKLTKKYNADIITVYVPSLDISSTYIREQLNEGKSIRYLVPENVENYL
                     YENKLYQYGDD"
     gene            44841..45413
                     /gene="yqeK"
                     /locus_tag="KRL67_000272"
     CDS             44841..45413
                     /gene="yqeK"
                     /locus_tag="KRL67_000272"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     YqeK"
                     /protein_id="HBH1521181.1"
                     /translation="MNLENINQRLNQMLPVGRLSHSKNVAKCAEKLCEIYGCDKEKAY
                     LAGMIHDCAKYLSDKEIEDYVNKYEIYLDPIEDGNRSLSHSVIGAYICEYEFEVEDED
                     IINAIKYHTTGREDMSLLEKIIYIADLIEEGRKFPVVDTLRELAYGGKLDEALLTSFN
                     NTLMFVINKKEEIHPRTVMARNYLIKEKLL"
     gene            45530..45880
                     /gene="rsfS"
                     /locus_tag="KRL67_000273"
     CDS             45530..45880
                     /gene="rsfS"
                     /locus_tag="KRL67_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HBH1521182.1"
                     /translation="MTVEQMTKIAYDAIEDKLGQDTVIINIGKVSSLCDYFIITTASS
                     QRQVKAIADNVEDELAKLGLEPRGKEGQGTQTWVLLDYGDIMVHVFNEENRGFYNLEK
                     LWKDAPYIDIDTLA"
     gene            46234..48654
                     /locus_tag="KRL67_000274"
     CDS             46234..48654
                     /locus_tag="KRL67_000274"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HBH1521183.1"
                     /translation="MSVYNFKEVESKWQKIWKDNNQYKMDTAQTEKPNYYTLEMFPYP
                     SGKIHMGHVRNYSIGDVVARFKKMEGYNVLHPMGWDSFGLPAENAAIKHGIHPHKWTM
                     ENIEEMKEQLNLLGISYDWDKEVATSTPEYYRFTQEIFLKFLEHGLAYKKKSYVNWCP
                     SCETVLANEQVVQGACERCKATVLKKDLEQWYFKTTEFAEELLNDLDTLDGWPEKVKT
                     MQKNWIGKSTGADLVFDIDGTDKSMTVFTTRPDTTYGVTYMVLAPEHELVKELVAGTE
                     YEADVEAFVKKMHTMTEIERTAADVEKEGMFIGRYVINPLNGKKVPLWIANYVLVEYG
                     TGAIMAVPAHDERDRDFAEKYNLDIIDVITEDNKMIDSEEFNGLDASEGFEGIIDKLE
                     KEGRGKRTINYRLRDWLVSRQRYWGCPIPVVYCDECGIVPVKKEDLPVLLPTDVEFTG
                     KGESPLTTSKQFMSTTCPHCGKPARREVDTMDTFVDSSWYFLRYVDSNNENEPFSKEL
                     VNRWHPVDQYIGGVEHAIMHLLYARWFVKAFKSMGMVDFNEPFKNLLTQGMVLMDGSK
                     MSKSKGNTVSPMDIIDEYGADTARLFVLFAAPPERDLDWSEQGVDGCFRFLNRVYRLV
                     DELADVVKKDVEFGELNSQDKDMRYTIHSTLKKVTADLSEKFGFNTAISALMELINDM
                     YKYKELDNINEAVIKEGVQTIVTIIAPFAPHLGEELWTMIGKEGSVFDIDWPKYDEKA
                     LVKDEIEVVVQVNGKVRGKLTVNSNISKDEMEKVALEDEKIKGLVEGKTIVKVVAVPK
                     KLVNVVVK"
     gene            49054..49437
                     /locus_tag="KRL67_000275"
     CDS             49054..49437
                     /locus_tag="KRL67_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBH1521184.1"
                     /translation="MSQKELAKKIGVSTSQLIKIISRKTRMVSSGILIGVADIFKFGL
                     SEDIIKELVIGAVDVEILNRLMEHKNFQCLINLMRIYSQDMVDLGDMERNQFIDMVTI
                     FLSNLMKESPKYYTEVRKELTDGIK"
     gene            49536..50204
                     /locus_tag="KRL67_000276"
     CDS             49536..50204
                     /locus_tag="KRL67_000276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HBH1521185.1"
                     /translation="METVSNNQSGVEIQLSLFEAIKDLLLINPIFLLGIILIGISLIV
                     IFKKDSDIPKVKTSILSLAMYYYLCLTLSHIVGIPTFSEYIRLSQLGETFFNPNISFI
                     PFSDGFSLSFILNILLFVPLGFLCPLISKTFERLRNTFLIGFGLSLFIETIQLFTLHR
                     ATDINDLITNVIGTIIGYFCFKLIAKLRIVKLHSDDDSMEQDYTVCIPIAIIIVALIL
                     GFFS"
     gene            50244..50324
                     /locus_tag="KRL67_000277"
                     /pseudo
     CDS             50244..50324
                     /locus_tag="KRL67_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005541856.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
     gene            complement(50687..50872)
                     /locus_tag="KRL67_000278"
     CDS             complement(50687..50872)
                     /locus_tag="KRL67_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HBH1521186.1"
                     /translation="MRIHDLRHTSATLLLLGGTNMKVVSERLGHTDIKITMNRYSHVL
                     KEMDKEASDNLSELLFK"
     gene            complement(51190..51612)
                     /locus_tag="KRL67_000279"
     CDS             complement(51190..51612)
                     /locus_tag="KRL67_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH1521187.1"
                     /translation="MTFVERCYKYIDMNKNNWSPYTVTNTKSWVKNYIEPFFKNLKLT
                     EVNPSSVQAFVNYNFNNSTFTSAKVRYSFLSSVISEVYRLREIQENSCDFIKLPSKEK
                     YSTNEIYNREEVLLLIEKLLGNNIEIPILLMLLLGLRM"
     gene            complement(51631..51729)
                     /locus_tag="KRL67_000280"
                     /pseudo
     CDS             complement(51631..51729)
                     /locus_tag="KRL67_000280"
                     /inference="COORDINATES: protein motif:HMM:NF026008.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Arm DNA-binding domain-containing protein"
     gene            52011..53381
                     /locus_tag="KRL67_000281"
     CDS             52011..53381
                     /locus_tag="KRL67_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp29"
                     /protein_id="HBH1521188.1"
                     /translation="MELKKLLSLGLAVSMFAAGPISANALDTIYTIQGKDKYETAALI
                     ADEQNYTTAILINTDSTMADGLSASGLAGATNAPILLTKKNNIPNATLKRVEKAKKVY
                     IIGGESSIDKATETLLKDKGIEAKRLQGSDRIKTSYNVAKEINSINKVNKVILTNAFK
                     GEPDAMSAASVAVRDKAAIVLTDGKSVPFNTTGVESYVIGGTSSMSDKLVNDTNSTRL
                     GGVDRYDTNKKIVNKFYNGAKEFYITSGTDLVYALVASPMSKNNPIVLVDIGSNKDIL
                     KNAAKVTGIGNLSDKIMLECENTINNISLPSSNKNIICGLKIDFDSKHIIWQDKGSYL
                     ECYDDNLEYYTEKYKDPTVPINIENTSTQNLDVTKLKVYGEANSTLYMGQYAKIKMDF
                     NSFGVLKPGEKCTIYLDIISLDPSRSMTLTFSYVGDKENYKYEQGLASRILFYNPDGE
                     DFFEYK"
     gene            53739..53897
                     /locus_tag="KRL67_000282"
     CDS             53739..53897
                     /locus_tag="KRL67_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH1521189.1"
                     /translation="MDNFLQGILASLSASLIVYVASKLFRKRKKPLKAATKSGWEFDL
                     KIRFHKTK"
     repeat_region   54182..54470
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=54183..54205
                     /rpt_unit_seq="gttttatattaactatatggaat"
     gene            54767..55027
                     /locus_tag="KRL67_000283"
     CDS             54767..55027
                     /locus_tag="KRL67_000283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HBH1521190.1"
                     /translation="MDNLISFIPEQLLLLVVALNVLGFGFKKYKQLDNKYISVILLAL
                     GIVFSIWMLGFNPSSILQGILCWGVAIGANQVYKQLKDGEKQ"
     gene            55024..55821
                     /locus_tag="KRL67_000284"
     CDS             55024..55821
                     /locus_tag="KRL67_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HBH1521191.1"
                     /translation="MKIAIVPGHTLSGKGTGATGYIDEGKENRILTDLIVKWLKQGGA
                     TVYTGKVDKSNNYLAEQCQIANKQNVDLAVQIHFNANKTTLNPMGTETICKTNNGKVY
                     AERVNKKLSTIFKNRGAKSDVRGLYWLSHTKAPAILIEVCFVDSKADTDYYIRHKDIV
                     AKLIAEGILNKKIDNDGVKLMYKHAIVYDGEVDKIPATVVGWGYNDGKILICDIKDYI
                     PGQTENLYVVGGGACEKIGSMAKENYTLIKGNGRFDTLYKALDFIDR"
     gene            complement(55843..56004)
                     /locus_tag="KRL67_000285"
     CDS             complement(55843..56004)
                     /locus_tag="KRL67_000285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic lactone autoinducer peptide"
                     /protein_id="HBH1521192.1"
                     /translation="MKKILYNFMKACGVLAMFVAFLSANTTSLWHVYQPKTPEKLKNS
                     KNHQKEGNN"
     gene            complement(56022..56603)
                     /locus_tag="KRL67_000286"
     CDS             complement(56022..56603)
                     /locus_tag="KRL67_000286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory gene regulator B family protein"
                     /protein_id="HBH1521193.1"
                     /translation="MIKSCADKIAFLFVLNQVIEKDDLEICSYGLEILLSSIINTLSS
                     LMIGLILGRLIYSIVFLISFCIIRQFSGGYHANSNKNCILIFNSIFFISMVLGTILNN
                     SNPIPLFLVILSSFNFLIIFIFAPVCHTNNPLNETKYKKNKLLSRTISITLFLIILLG
                     CKYLSFYNYVVYTFLALLWINLLLIIQIIINKK"
     gene            complement(56646..>57141)
                     /locus_tag="KRL67_000287"
     CDS             complement(56646..>57141)
                     /locus_tag="KRL67_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HBH1521194.1"
                     /translation="LDEYSAVFDTGNITLNIILLEKKELCIEKGIDFTININFSDCNF
                     IKEEDTCSIFANALDNAIEACEKIKDDDKKIKLQGMVLNKFFVIKITNTKSNDILLKN
                     NAFLTTKEDKEFHGLGIKSIKNSLEKYDGAISINFSENLFCLNMMIPIRKSQEDSNIA
                     YENS"
BASE COUNT        22117 a         6326 c         9776 g        18922 t
ORIGIN      
        1 tttatccaaa aacttttcta ggtttaaatt ataccatatt tatagtatga tatgattata
       61 attttatata ataaataagt aaaaatagta aaaaataaat taagaaatgt ttttaataaa
      121 aataagctta agggggtcaa ccatgagtgc aaaggtaacg aatcaatatg gaaatataga
      181 aatagataat caagtaatag ctcaagtcgt atatagagct gcaatggaaa gttatggctt
      241 agtaggttta gcatataagt ctaaaggaat agtagagcta ttaaaaggcg agaacgcaac
      301 taaaggtgta agagttgagg aagtagaaga agatgccata gcaatcgaac tttatgttat
      361 aatacaatat ggtacaaata tatctactgt tgccaataat ataatagata gagtaaaata
      421 tgtagttgaa aaaatgactg gcgtaagagt tactagaatt gatattaatg tacaaggaat
      481 aagagttaag taatttcata ggaggaaaca aatgattcag tatatagatg gtaaaaggtt
      541 aagggagatg tttatttcag gtgcaaacaa tctccaaaac aataaagatt tagttgacaa
      601 gttaaatgta ttccctgtac cagatggaga tacaggaact aatatgtcat taacaatatc
      661 ttatgcctta aaagaattag caaaagttga aaatgataat atatcagata tcggaaaagc
      721 attatctaaa ggctctttaa tgggagctag aggaaactca ggagttatac tttcacaaat
      781 aatcagaggt atagctaaat ctatagaagg aaaaagtaaa ttatcaacag aagatttagc
      841 aaaagctttt aaaaatggtt cagacacagc atataaagct gtaataaaac caattgaagg
      901 aactatactt actgtagtta gagagagtgg agagtttgca ataaaaactg cgaaaaaaga
      961 gaaagatgta gtaaagtttt taagtatgct tgtgaaggaa tccaattctt cattagaaag
     1021 aactccagac cttctaaaga atctaaaaga agctggtgtg gttgactctg gtggaaaggg
     1081 tcttgtactt atatatgaag gaatgttagc ttctataaaa ggcgataaca tagaaataaa
     1141 aaatgctgac ctagatacta atatttcaac aagtatggat tttgcaaaga gtactactag
     1201 cacagatgat ataaaatatt gctattgtac agaatttatt ttagaaagtt ctaaggtaga
     1261 agataccaaa ataagagaca taatgatggc ttatggtgat agcttagcag tagttggaga
     1321 tgatggagtc ataaaggtac atgtacacac taatgaccca ggaaatgtac ttcaagaagc
     1381 tctaaagtac ggtcaattat taactataaa aatagaaaat atgaagttac aacatgaaaa
     1441 tacattactg gatgtagaag aaaagaaaga aaatgatagt gaacctttag aagaagaaaa
     1501 agagtttggg tttatagcta cttctatggg tgaaggtttg gcaaatatat ttaaagattt
     1561 cggagttgac catataatag aaggtggtca aactatgaat ccaagtactg aagactttat
     1621 gaatgctata aaggatataa atgctaaaaa tatatttatt ttcccaaata acagcaatat
     1681 aataatggct gctaatcagg caaaagaatt gagtgataaa aatataatag ttatacctac
     1741 aaaaaatacg cctcaaggat ttgctgcatt agtaactttt aatggagaat taagcgaaga
     1801 tgaaaataaa gaagctatga tgaatgcttt aaactcagtt aaatcaggtc aagtaacttt
     1861 tgctgttagg gatacagtga tgaatgaaat tgatgttaaa gaaggtaata taattggaat
     1921 tgcagaagga aatttattat cagcaggaga ttatgtggat gaagtaacat caaaccttat
     1981 tgaaaagttg gttgatgaag atactgctat aataacatta ttctttggtg aagatgttac
     2041 agaatctcaa gcaaatgaac tacgtacttc tttagaagaa aagtttgaag atgtagatgt
     2101 agagttatat tatggaggac aacctcttta ttattactta atttcagttg aataatgatt
     2161 ataagaacct gtatgaccaa gtagctttta taaaattggt tgtacaggat ttttgtgtag
     2221 taggtgatat atttgttgga tttatataaa gatgttcaat atgttaaggg aataggacct
     2281 aaaaaagcag ataagctaaa taagctgggt atatttacac taaaagacct attgtattat
     2341 tttccaaggc aatttgaaga tagaaataat ctaaagaaaa tagcacaatt agaagatggt
     2401 gagaaagtta ccataaaagc agtaatatca agtataaata catttagccc aaaagaaggt
     2461 atgactttaa caaaaattga tgtaaaagat gagacaggct cggctaaatt agtgtttttt
     2521 aataagagtt atataaaaaa tacttttcgt ccaggagatt caatattagt ttttgggaag
     2581 gttaagaaaa aatttaataa cttagaactt acatcatgtg aattagaata tcttactaat
     2641 tcacctaaga atacatgtag atttatgcct gtgtaccagc ttacatatgg tgttacaaat
     2701 aaagaaatta tgagtataat tagaacagta cttgaagata aagacttaat tatacaagaa
     2761 tatatgccac aaagaattat agaaaaatat aggctatgta gtatagattt tgcagttaga
     2821 aacatacatt caccaagtag taaggaatca ttaaaaatag ctttatatag aattgtattt
     2881 gaagaattac ttatactaca actagggttg tttgtattta aaagtggcag aaataaagaa
     2941 gatggaataa aatttgaaac aagtaaagat ttaaaaaaaa taataagtgc tttaccattt
     3001 aaactgacta aagctcaaaa tagggcatta gatgaaatta tacaagatat gaatttagag
     3061 aaaataatga acagattggt acaaggtgat gtagggagtg gtaaaacagt agtagctctc
     3121 ttagcacttg caaattgtgt tttaaatggt tatcagggtg ctttgatggc acctacagag
     3181 atattagcag gtcaacatta tatttcactt actgagtctc taaaagattt tggaataaat
     3241 gtaggtctac taataggtag tcttacaaaa aaacaaaagg atacagtatt ggaacaaata
     3301 aagaataatg agattgatat attaataggt acacatgcac taattgaaga taaggttgaa
     3361 tttaataata ttggtttagt tataacagat gaacagcata gatttggtgt tatgcaaagg
     3421 agcaaacttt cattgaaagg agcaaatcct gatatattag taatgacagc aacaccaata
     3481 ccaagaactt tagcacttat actttatgga gatttagata tttctattat agatgaactt
     3541 cctccaggaa gacagccaat agaaacaatt gctattgaaa aaagtaagag agatagagca
     3601 tataataatc ttgtaagaag agaggttgaa tcagggagac aagtgtacat tgtatgtcct
     3661 ttggtagaag aaagtgaagc catagaagcc aaatcagcag ttgaacttgt tgaggagtta
     3721 agagcagaat attttcacga cttaaggctt gggttgcttc atggtaaaat gaagtcaagt
     3781 gaaaaagatg aagtaatggg actttttaaa aataaagaaa tagatatttt agtttcaaca
     3841 acagtaatag aagttggtgt caatgtgcca aatgcaacat taatgataat agaaaatgca
     3901 gaaagatttg gacttgcaca attacatcag cttagagggc gagttggaag aggaagtcat
     3961 aaatcgtact gtgtactaat ttatgattct aaaacagatg tatgtagaca aagaatggct
     4021 ataatggaag agaccaatga tggttttaag atttcagaga aggatttaga aataagagga
     4081 cctggggaat tttttgggac aaggcaacat ggtcttccag aacttaaggt agcaaactta
     4141 tttaaacata taaaaatact gaagttagca cagcaagaag ctcgatatat attgggtgaa
     4201 gataataatc tacaattaaa agaaaacatg gcactcaaaa aagaaattat agataaattt
     4261 aaagatacat tgaaagaaat ttcattaaat taattttaaa ttgtgttaaa atattactaa
     4321 tgtaataatg tgctgaaggg agattgtcat attgagagta atttcaggaa aagcgagagg
     4381 attaaaacta aatactccaa aaaatgaaga tgtaaggcca actacagata gagtaaaaga
     4441 gtctctattt aatatgataa attcatatat aatggaaagt gaagttctgg atttatttgc
     4501 tgggactggt tctttaggaa tagagtgttt atctaggggt gccaagtcat gtacttttgt
     4561 agatatcagt aaggaaagca tagatattgt taagtccaat ataaaaaagg caagagttga
     4621 aagtgaaagt attattttaa acctagattt taagactgca atagataaac ttaaattaca
     4681 aaatagtaaa tttgacataa tttttatgga ccctccgtat tataaaaata tgtttataga
     4741 agctattgaa aaaattgata attctaatct attgaacgaa gatggaataa tagttgtaga
     4801 acatgatacg aatgatttat ttccagataa gatatgtaaa ttggagaaaa ctagagataa
     4861 gaaatatgga aatactacat taacttttta taagatggag gcataatatg gaaaataaac
     4921 ctagaaaagc tatatttgca ggaagttttg acccaattac taatggacat ttagatatta
     4981 tttgcagggc ttcgaagtta tttgacgaat tacaaatagg agtattaaat aatcctaata
     5041 aaaagggtct atttagcttt gatgaaagag taaaacttat agaaaaaagt acaagccatt
     5101 taaataatat aaaagttgtg acttttgatg gtttattaat aaattactgt caggaaaatg
     5161 ggattggagc attagttaga ggtgtgagaa gtggtgctga tgttgattat gaacttcaga
     5221 tggcgcatat gaatagagag cttaatccag atatagagac tataatttta ccaagttgta
     5281 caaaatattc atttataagt tcatctttaa taaaagaggt tttgctcttt gatgctgata
     5341 taaaaaattt ggttccaaag attgttttgg aagaattaaa gaaaaaaact agtgggggta
     5401 attgatatat gaagatagat ttagaaatga ttgaactatt tgagcagtta gaggatatat
     5461 ttagaagtgc ttctacaatc cctttctcac ataaatgcac agtggataaa gatgaggttc
     5521 tagcaataat taacgatata agaactttaa taccagaaga agtaacacag gcagtatgga
     5581 ttaataaaga aagaaataaa ataataagtc aagctaagca agatgctaca aatatagttg
     5641 aacaagcgaa aaaagaagct gagagaatac aacaagaata tcaagataat atagaggaat
     5701 taaagaaaaa ttctgaagat gtagtaaagg catatgtaga atccagtgaa cctgttgttc
     5761 aagcagacca aagagcaaaa gatatagttg atagagctga gagaatagcc aatgagataa
     5821 gaattgggtc aattgaatat gcagaggatg tgctttctag tgttgagtat aatttaagag
     5881 aaatattaga agaagtaaaa agagataaag ctgaacttag acctaaaaaa taaattttaa
     5941 ataaataaaa atggagctat cacacaaaaa attccaattg atgtgtgata gctctctttt
     6001 aatatagcaa ttttttgtta cctaattgtg cttttcagat tacttatatc ttattttgat
     6061 attataatgt agtttaatct tttatttgtt gaaatcaact tatttctttg tataaataat
     6121 ataggctatt ttcaaatttt tttaatactt ttaccactgt attcttttag attagagcta
     6181 gactgtgcaa aattataaaa taatactaaa tcatgtgcaa ttatttggta tggcactaca
     6241 tatagaaaat atagataata aaattaagga ggtagtatga aggaaagggc taaaaaaata
     6301 ttagatttta ttattaaaaa taacaatgta acatcacaag aactacaaga aaagtttaat
     6361 gtttcaaaga gaactattta ttacgatatt ctagctataa ataaacaact aggaaaaagt
     6421 ggtaatataa aaaatgtaaa acacaaattt atatttgaag gtaatttatg tgatgcaaga
     6481 aaaataattt cagctgaaga agataaattt ttagatagtg attacagaaa aacttttata
     6541 ttaaacaaga tattacttgg cgaaaaaata tcaatagaaa aactaacaaa tgaaatgttg
     6601 ctttcaaaaa acacagtagt acaaacaata actgatgtaa aaaaatacct tcaaacaatg
     6661 gggttaagat tagagtataa gggaaagtac aaaattatag gtgatgaata cgttattaga
     6721 gagctgtttc tgataattgt tcaagaaaat gtactggaaa ttaattctat aagtaaggaa
     6781 gtaagtagtt tcgacacaaa aggacatata aaacttactg attattcttt attaaatcta
     6841 actaagtttg tggaatttct aaataaaagg ataagagatg ggaaaacatt atactcatat
     6901 aaatatctaa atgaagctaa aaaaattagt tactttagta attgtaaaga acttttatgt
     6961 gaagaagcta atgaaaatga acaagcctat atatgcactt acataagttc acttcccagc
     7021 ttaaatagtg aagttaagga agatgttgtt gaagaatatg tagataaact tatagataag
     7081 tttgaagtaa atacagcaat aaaattagaa agtaagcatg agtttaagaa aaatatttta
     7141 aggcacctcc actcttctta caacagaata cgatttaagt ttcctattag aaatccaatg
     7201 ttagatgaga ctaagtataa acatgagtca ctttataaaa ttataaaatc aattatagaa
     7261 aacgaagaag agtttcctgt atttgagggg ataagagaag aagaaatagg atttatagca
     7321 gcgtattttg gagggtattt aagagggtct agggataatg gtctaagaag aaataaagtc
     7381 ttactagttt gtcctaatgg tcttatggta tcaaagagtt tggaaataca actatataaa
     7441 tacataccaa ctatagaaat agtaggtata gtgtccatta agcaactaaa agaagtaaat
     7501 gtatattacg attacataat tacaactata gatatacaaa atgtaaacaa tgtaattgtt
     7561 gtaaatcctc ttttaacatc atcagatgtt caattactta tgaacaaact aataagtgta
     7621 aaagaaaatg agaagtattt caatcttgaa ttaataatac aagctataag aaaaaatgga
     7681 gttatcaata atgaagaagc actaaaagtt gatttattaa acataataca caagatagat
     7741 gaaggagaga tgtatcaacc aatgttaaag gaattaatta atgcagaaag ggtcaatata
     7801 ataaaaagtg ttagagattg gaaagaagca ataaaaatag catcaaagcc attgttagaa
     7861 gataactcta tagaagagtt atatatagaa aatatgataa aatcagttga aaaatatgga
     7921 ccatatattg ttttagctga tagatttgct ctaccacatg caagttctaa agaaggagta
     7981 aacaagcttg ccatgtctct attgatagta gaagatgaag ttgatttatt aggaaaacca
     8041 gtaaatatat ttatggtatt agctgctgta gataacacta ctcacattag agcattagca
     8101 tcactatcag agatgatgta cgaggaagaa aatgtaaaac taataattaa tggtgataaa
     8161 agttcaatac tagaacttat aaataagcaa aattaggagg aatgaaaaat gaagatatta
     8221 gcagtatgtg gttttggtgt aggttcatcg atggtattaa aaatgacaat acaaaaggct
     8281 ctaaagcagt taggtataga atgtgaagta gaaaatacag atataaattc agctagaggc
     8341 gctgactgtg actgtatatt cacaagtcaa gaattgctaa atgaattaaa aggaacttgt
     8401 aatgtagagg tatatccagt taaaaagtac atggatttag cagaagtaac agaagctgtt
     8461 aaaacttttt taagtgataa aaatgataag aaatagtata agttttaaaa agtaaattta
     8521 agttaatcag aataaaataa aaacttacta aatttaaaaa tagcaagaaa gagaggaggg
     8581 atttagtggt agaaaactta aacagttgac ctatcactaa aaaatataat gcttgatttt
     8641 atttcaaata atattttacg taatccatct atgttaattg gattaatagc agcatttggt
     8701 cttgtattac agaagaagaa agcatctgat gttataaagg ggtcactact tgcagctttc
     8761 ggaattataa ttatggagac tggaacaggt atgttagtta gttcaatagc accaattaat
     8821 ggagccttcc aaagtataag tggtggtgca gtaacaaaag gtttaagtga tgttactttt
     8881 actgcaagtt atggtggaat tataggttta gcaatgtttg tgggtttagc tattcaccta
     8941 atgatagcaa gatttactcc tattaagaca atcttcttaa caggtcacat gctttattgg
     9001 ttcccattca tatttgtagc ttcaggagtt gaagctggat taaatggtgg agtattaata
     9061 gcttttgcag caatattatc agcactctat tggtcaataa tgccttggtt attaagaaaa
     9121 tatgtgtttg atgttatagg agatgattct tttacattag gtcatccatc tggttgttta
     9181 gcacttatat caggatttgt tgctaaatta gtaggaaata aagaaaaatc tacagaagat
     9241 ataaacatac ctcaaagttt atcatttttt agagaaatat ctataagtgg tggaatggca
     9301 gtgtttttaa tgtttatagt tgttgggata gtaataccag gtgcgtttaa agatagtaat
     9361 gagtttattg gaacgtatgc aataaagcaa ggtctttctt ttggagcagg tcttataata
     9421 atgcttcatg gggtaagaat gctaataaat caaataatgc cagcatttca aggtatatct
     9481 gaaaaagttg tgccaaatgc actacctgct tttgactgtc caatattatt taactataaa
     9541 ccaaatgctg tattaatagg attcttagtt gctatgctaa catctacagc tatgatttta
     9601 atatgtaact actttaatgt atttaatttg ttacttatac cacttgttat aacttcattc
     9661 tttgagtgtg gagcagcagc agtaatagga gaatcacaag gtggtatacg tggagctata
     9721 ataggtacag ctatagctgc aatatttatg gtagttttag taggaatatc tgctgtaatt
     9781 tactcaggaa ctattcaaaa ttggatacta atatttggag gtaacgacct ttcattattt
     9841 ggtataatca gtaagtttat aggttcatta tttggaggaa tattatagat atgaaattta
     9901 aatacataga tgagataaga agattaattg atgtaataga aaacgaagaa aaagtcaata
     9961 tggaaaaagc agtacaaatc cttgttgaag caatagaaag caaaaatgct atattttcat
    10021 ttggagcttc acatgcagga atattaacag aagaattatt ctatagagca ggtggctgtg
    10081 ttattataaa tccagtattt gcaaaatctc taatgttaga tacagctcct ataactcaca
    10141 ctagcaagat ggaaagatta gaaggatatg gtacagctct agctgataag actccaatta
    10201 aaaaaggaga tgtagttata gttcattcag tatctggtag aaatccagta agtatagagt
    10261 ttgctatgaa agctaaggaa aataaggcaa tagtaatatg tataactaat ttaagttact
    10321 caaaaagtgt atcttcaaga cattcttcag gaaaaaaact ttatgaagta tgcgatttag
    10381 tgatagataa tcatggtcaa aagggagatg cctgtgtaga gatagacgga ttaaaccaaa
    10441 aggttagtcc aacttctact gtaatagcaa ctactgtgat gaactctatt atagctcaag
    10501 ctactcagga attagttaat agagggttaa agaatcctcc gattttctat agtgccaata
    10561 ttgatggtgg agatgagtta aataagaaga tttttgatga gtataaagat gtaattcatt
    10621 atgaatacta aggtgaaaaa agtgtgttaa taaactttga agtttatcaa cggagaatat
    10681 taataatata taataaatgc tatcatttaa aagctgtagg taaaaatcta cagcttttta
    10741 ttattgaata ttaaaaagat gtgtaattaa attttaaatt ggtgaaaata aattgaaaga
    10801 tatcaattat aaatattaga aacgttgtca tagaaaaaaa ctatattaaa aaaaagtaca
    10861 tagaaaaaac tgattagaca ataaatacat gctttgataa tattttaaat ggagtgaaaa
    10921 tgtgtagaat tataatgaaa aaagaaaata aaaatgaaaa aagaaaataa aatggaggga
    10981 ataaaagtgg gtatgttaaa aaaattatgt tcactagcta tggtagcaat tatgactaca
    11041 acacttataa cagggtgttc aagtggaaat ggaaaagatt caaaaaagga tggcgaaaaa
    11101 gaaaaactag ttctttggat gccaccagta gaaaataata tgaaggaagt ttgggaccct
    11161 attttagata aatttgagga aaaaaataac tgtgaagtag atttacagat aattccttgg
    11221 gataattatt ctgaaaagtt tgcaacagct ataagtgctg gcgaaggtcc agatgtagga
    11281 tatatgtatg cagaaatgta tccacagttt atagaaagtg gagcagtaga agatttgact
    11341 aactatgcaa ctaaagagga taaagagcaa tctatttata taaaaacatc tgaaatgatg
    11401 ggtggaatgt atggtatgcc attccaagca gcaaatccag gggtattata ctataacaag
    11461 gatattttag agaaattagg agaaaaacct cctaaaacat gggaagattt taaaagaatt
    11521 tgtaaaaaag caacacaaga cactgatggt gatggaaaga tagaccaatg gggattagct
    11581 cagggatggg gagctaaaac ttttggtaac ctaaattgga actggtatcc ttatttatgg
    11641 caagctggtg gagatatctt taatgacgat ttaaaatcag ttaaatttaa tgataagagt
    11701 ggtttagaag cagctaattt cttaaaagaa ttacaagcat acgtacctga agattctcta
    11761 tctaaagata gtaatgaaat gatagaaagt gtatttggac cagggaaagc agcatttaca
    11821 ataatgttat cctctgctgc aacaagtgta tttgatgaat cattcccaga tttaaattgg
    11881 ggatttgtaa ctggactaga agataagaaa gcagcaactt ttggagcagt agaccattta
    11941 tctttaatgt catctgcaaa aaataaagaa cttgcataca agttaataca acatatgtta
    12001 agtgttgaat caatgacaga atttcataaa gcaataccaa gagcaccaat aacaaaaggt
    12061 gaaccatatc aaggtgatga aagattcaaa gaaatggtgg aaaatgacaa agatgtatac
    12121 agaccattag tagtaggacc tcatggagtt gaaatatatg aatatttatg gaaagaactt
    12181 caaactatga tttcaggaga taaaactcca aaacaagcgt tagatgatgc tgcaaaatat
    12241 tcaaatgatt tattagcaca ataaaagttt aaagctgtaa tatgttgtaa agtactttac
    12301 agattaaaat tagcattgta tttataaata caatgctaat atttatattg gagaggataa
    12361 aatggaaaac ttagttagag agtttgaaga aaattcaaga agaaaaaaaa caaaattaaa
    12421 tattaaacca tatatgtata tattgccatt gggaataatt ttagtggcgt tttatgtgat
    12481 accaataata atgtcaatat actttagttt tacaaagtat aatattatta gtccagctac
    12541 ttttataggt ttagaaaatt ataaaaaatt atttacagat gaaacattaa aagtatctat
    12601 tataaatact attaaattta ctgttgttgt agtaccttgc caaactatat tatcattgat
    12661 tttggcagtt tggattacag gaaaaggaaa tagtaaaata gcttcatttg ctaaaggtgc
    12721 tatatttata ccagtattat catctatggt tttgataggt atggtatgga gggctttatt
    12781 gaatggagaa ggttctatta tatatcaggt attaggtgcc tttggaatag aatcatctaa
    12841 acttttagga gatagtaaga cagcattacc tacacttatg tttatatcta tgtggaaaaa
    12901 tatagggtat ttcatggtaa tttatatttc agctataatg aatttaccaa aacactgcta
    12961 tgaagtagcg aaggttgatg gggctacaaa acttcaagaa tttataaaaa taactgtacc
    13021 tttattgaaa ccaactacaa taatggttgt atttttaggg tcgatatggt cattacaagt
    13081 atttgactta gtttatactg tgactggtgg aggtccaggt atatctacta tgagtatagt
    13141 tatgcatgca tttaacttaa actttaaaaa ctttaattct ggatatgcaa tgactgtagc
    13201 aaacgtatta tttttgttaa tagcggtagt ttcgatttta caaaataagc ttgtaaaacg
    13261 agacaattca gatttttaga aaggtaggta taatatgaaa acagcaagaa gattattaag
    13321 tgatggttta ctcttattta tagcgtgtgc aagtttggta ccttttatat acatgttgat
    13381 aatatcattg aagataacat ataactcata tagtttggat atatcttttt caacagtaac
    13441 ccttcaaaac tatatagata tctttactaa aaaaggattt gcacaatatt ttttaaatac
    13501 agcaatagta tctttttcag gtgtactatt aaatttagta tttagtacat tagcaggata
    13561 ttcttttgcg aagatggatt ttaaaggtag tgataagtta tttttattta tgataatgac
    13621 tttaattata ccttcccaag ttacaatgat accactatat ataataatga aacacttagg
    13681 atggataaat agttatcttg cacttattat gccaatacca actgcatttg gcgtatttat
    13741 aatgagacaa gctattttag gagtaccaaa agagttatta gaatcagcaa agatagatgg
    13801 ttgttcggat tttagaattt taatacaaat agtattgcct ttaattaaac cagcattaat
    13861 tacactagca atatttactt ttatgggagc atggaacgag tttatgtggc ctcttatagc
    13921 tacaactaaa gatgcaatga gaacattaac agttggatta tctactctta agacattcca
    13981 gataactaat tatggacaga tgatggcagg ggctacaata acatttttac ctccatttat
    14041 tttttatttg attttacaga gtaaatttgt tgaaggtgtg tctttgtctg gtataaaagg
    14101 ttaaatagtc taatttaata atatataaag aaacttatat aaatatggag gaatttaaac
    14161 tatggaaatt tcatatgtac tgaaagattg cacattcaaa cacaataaat actcagttat
    14221 agaagctgaa acttggaaaa ataaggattt gggtattgta gtagctaaag gtgaaaaatt
    14281 tgcatttcaa attatgctaa aagcaacaga agaatttaat tgtacaattg ataaaagcag
    14341 tactatatca tggaaaggtc tgggaaatag agtaaggtta gcattaaatg tgcctaatag
    14401 tttagaaaat aattttagta taaatatatt aggatatgtt caagatgata caaaagcatt
    14461 tgtgaatgat gctattttaa gggataaaga tgtattagta gagcaatatt tgcctcaaac
    14521 attttggatt gaaggtcaag taccagaaga ttttgaagaa aataaccttg atataggtat
    14581 tgatatattt aaaagctttg gatatgaaga tgaagaaaaa gtttgtacaa ttgatgttcc
    14641 tgtaaaagta agaaatgttg tgctaaagcc tttagatgaa agcaagttct ttttagattt
    14701 atggcaacat ccaagctgtt tagctagaat gtataaagtt gagttatggt ctgatataca
    14761 ttttgaaata gtcgataact atttaaaaga attagcatct ttaggagaaa aagttgcaac
    14821 agttatagtt tcagattatc catgggcagg gcaaagttgt tataaagttt ataaaaatcc
    14881 atcaaactta tatgaatata acatggtaag tgtatcaaaa ggattggatg gaaagattaa
    14941 atgtaacttt gagagtatgg ataggtatat aagcatagct gataagtata aaatggctca
    15001 ggagatagat ttatttggtc tattaggtaa ttggtgtgct ggtgagtttg gaaatcctgt
    15061 agaaggttat aaggacccta taagagtaag atattttgat gaaaatgata aagtatttaa
    15121 atttataaac aatacaaatg atttaaaaga atatatagga ttattgttaa atcatttaat
    15181 agagtgtgga ttgtgggaca gagtaaggat tatagctgat gaaccaaata atccagaagt
    15241 agtcaaagag tgtatagaat ttataaattc tactgtaggt actcatcaag ttaaatataa
    15301 atcagcaact cacgatcaaa actttttaga tagagctaaa gatgaaattg atgatatgtc
    15361 aataaattta aaacttacta tacaaaatta taaagatata gaaagtctta agaaaaaaat
    15421 caatgacaag ggtgggatat tgacttggtt tgtatgctgt tttccagaga aaccaaatag
    15481 ttttttaagt tctccttttg tagaaaatag aataatagga tggtatactt attactttgg
    15541 tcttgatggg tttttaagat gggactataa tttatggaca gaagaccctt ggaaagattc
    15601 aagttacaaa ttccctattt ggaaagctgg agatatgttc tttgtttatc ctggaaaaga
    15661 tttaaagcca gttagaagtg tcagaatgga gaatttaaga tttggaatac aggactttga
    15721 gctatttaca atgttagaaa aagaaaaggg aagagagtat atagaagttg agcttatgca
    15781 agaattatta aataaaaaag aaaatgctga aattaaaggt tttggtgaca tagaactagg
    15841 atattcactt gataattgtg gatatgataa agttaaaaaa tatgtattag agttgttaga
    15901 cagagtataa aaaatataag ggtgtctcaa aatgaatttt ttagttcatg aggcactctt
    15961 tttgtatgaa atcaaatata ttttcatcgt ttcaataatg aaaaataggc ttatttctat
    16021 tctgagaaag ccctttagtt tttagctgta tttagtgttt taaaatcaac tataaacgct
    16081 ataaagttta taaatgacaa taattatatg taacatgatg tactttaatt tgctataata
    16141 gggattgaat aatattatat agaagataac attgtttggg gggattaaga taaaaagaat
    16201 aggtattatt ataatgtttt ttttaagtat agcttttatt ggattttatc ttgtaataag
    16261 agataacagt gatacagtta agatgcaacg aggaataaat ataggtaatg cactagaatc
    16321 acctaaggat tttccgtggg atgtaaaaat gtcaaataag ttttttgatg atataaaaga
    16381 tgcaggtttt gatacagtac gtataccagt aaggttttct gattatacgt cagattttga
    16441 taattttaaa attgacgaag aattttttaa aaagattgat aaatatgtag attatgcatt
    16501 ggataaagac ttaatagttg tgttggattt acaccatttt gaagaaatta tgaaagaacc
    16561 aagagttcat aaagaagagt ttttaaaaat atggcaacag atagctaata ggtatcaaaa
    16621 atatgataaa aaattggtat ttgaattatt aaatgaacct aaagaaaatt tatcttcaca
    16681 attactaaat gaatacattg aagaagcaat aaagataatt agaaaaacta accccaaaag
    16741 aactattata gtgggaccat ataattttta tcaaattgat tatttgaatg aattaaatat
    16801 tcctaaagat tctaatatag tagtttcttt tcactattat gaaccaaatg attttgcatt
    16861 tcaaggaaat atctatcata agggatttga acacttaagc aatattactt gggaaggaac
    16921 aaatgaacaa atggagtatc taaaaaaaag gtttgatact gtggaaaatt gggcaaataa
    16981 aaataaggtg aaaatcttct tgggtgaatt tggaataaca aaagaagcac cagaagcttc
    17041 aagaagagca tggataaaag ctgtaagaga agaagctgaa aaacgaaact tttcatgggc
    17101 ttattgggag cttgcttctg gatttggtat atacaatcaa attgaaggaa cttgggatag
    17161 agatatatta agtgcattaa tagaaaagag gtgattttat gaagaaaaat tcaaaaaaga
    17221 tacttataac tttaactatt attactaata taatatatat attatggaga attttttata
    17281 cagtacctaa agaagaaggt atgtttgcct taatttgtgc aataatactt ctttttgtag
    17341 agataatggg catgatggaa atgtttgtac attattatgg tatgtcgaat attgagtatc
    17401 ctgaaaaacc aatcataagt gaagaacttt atccacatgt agatgtattc attgcaacat
    17461 ataatgaatc tgttgatttg gtgagaaaaa ctgtaaatgg atgtattcat atgcagtatc
    17521 cagataagaa aaaggtacat atatacatat gtgatgatgg aaatcgtgaa gaaatgcgta
    17581 ttctagcaga aaaaatgggt gtaaattaca taactagaac tgaaagagaa ggagcaaaag
    17641 ctgggaattt aaataatgca atgcaacata caaattcccc tttgattgct acatttgatg
    17701 cagatatgat accaatgcat gactttttaa ttgctacagt accatacttt ttgaaaaatg
    17761 agcaagccaa aaaagatgga gaaaaagagg agtatgaaaa ggtcggtttt gtgcaaactc
    17821 cacagagttt ttataatcca gacttgttcc aatttaatct gcactctgag cgaaggattc
    17881 caaatgagca agattatttt tacagagata ttcaattggc tagaaatagg acaaattctg
    17941 taatttatgg tggtagtaat acagttattt caagggaagc acttgaagaa gttgatggat
    18001 tttatactta ttctattaca gaagattttg caacaggtat cttaattcaa agtaaggggt
    18061 atagatgtta tgctattcca gaagtacatg catcaggttt atctcctaca gatttaaaaa
    18121 gtcttattaa gcaacgtgaa agatgggcac gtggatgtat acaaactggt agacgtctaa
    18181 atattttgtt taggagaggt ttaggttttt ggcagaaaat aagttatatt tcttcaatta
    18241 cttattggta tgcaagtata aaacgttttg tatatataat ggcacctatt ttattttctg
    18301 tgtttaatgt gattgtagta aaatgtacat tacttcaagt attggtattt tggttgccaa
    18361 tgtatatttt aagtagttta tctcttaaaa tattttctca aaatataagg aatattagat
    18421 ggaccaatat atatgaaact ataatgtttc agtccttaat gccagcagta atacttgaaa
    18481 catttgcaat atctaaaaat aaattttcag taacaaataa gagtaaatta gaagaaaata
    18541 gaatgtataa gtttttacag ggaatacctt attttattta catggtctta tctatcatag
    18601 gaattttaaa aatgtttgta gcaatattta aaatgagttc aataacatat tcagttgtat
    18661 tattttggtt gattggaaac cttttcaatc ttgttatggc tacactattt atatcaggaa
    18721 gacaacagtt aagaaagtct gagcgttata ttgctgaaat tgattttaag ttaaagcaaa
    18781 attcttatgt tctttcttca aaaacgattg atatatcaga aaatgggttt gcgtttttgc
    18841 tagaaaatcc agaatatatt tccccagaag aagagtttga ggtagagttt agagaaaagt
    18901 ctggaaatga aatgtatata gctaatatga aagcaaagat tgttaatgtt gtggaagtaa
    18961 attcaaagtg gaaatatgca gcttatatta cacatattga agatagtgaa atagataatt
    19021 ggatgtgtat tgttcacgat agaataccta ctttaccgat gacaatatct aatcaactag
    19081 gtttctttga tgatttgcaa attaatgtaa aaaaacgtat tgaaaaaact agaacattat
    19141 cacgaagaag tccaagaatc aatatgaatt ttcagatgga tataaaaaat acaggtaaat
    19201 taagaatagt aaattttaat tatcaatatg tattattaaa ttttgagaat aaaaatattt
    19261 atcctaaaga aatagaattg gaaattaatg aaggtattgt attagaatgc gatttatgtg
    19321 agggtaaaat tgatgaaaga ggtatcttat atagagtaaa taacattgac agcataatgc
    19381 aaaatttctt tttaagagat gaaatgatgg attggatatt acaaaataag acaatttttg
    19441 tttcaaagcc tagtgaaaag aaggaaaaaa gtatagatga gtttgaaccc atggaatata
    19501 tttaggagag atgaaatgaa gactttaaaa gtgttttttt gttctgcaaa atatcttatt
    19561 ttggtgttta ttttttttat aaatactact tttataagtt cagcaacaag tgataaaatt
    19621 ttagatttat cctttaagaa gatagagact gacttaagtt ctaagattac atatgaagat
    19681 acaggtgtaa aaatagaaac ggacagttct aaaagtgata aggaacgtta tttatatatt
    19741 taccaaaata taaaagaaaa ttggagtatg tataataatt tttatattga aattcaaaat
    19801 aaaaataaaa gttcccagag aataaattta agtgtacaaa gtaagaatat gcttgaattt
    19861 aggttaaaag aaggtagtga aatatttttg gaagataaaa atatagtata ttcagataaa
    19921 ataaaagaag gatgtataga agtaccaggt gaatttgaag gcaaaattta tgtgaacttt
    19981 aatagcctta taaatgagga aagtaatgtt gtgctggact caaatatgct ttcaaacatt
    20041 gttagttggg gaattacgtt tattccatct gatgaagaac ataatattgt tattataaaa
    20101 aagatatctt tattgtctaa agaaaaatta gtatttttaa ataatattaa aataattggt
    20161 gatgaagaag tacaaatacc agtattgggt caaagtataa gtcagtatga ggtattaggt
    20221 cttaaaagtg attctaaaat taagtactct cttatgggaa aacaagataa tgtgagcata
    20281 tctcaaaacg gtaaattaac attaagtaat aaagccaaac ctggaaaaat tattttacaa
    20341 gttaatgttg atgataagtt taaaattgga aagaaaataa cattaactga gtcttggagt
    20401 gttaataaaa aagataagga tggtgttcca tatacattgg tatctcctga acaatcacca
    20461 acagtacaag atatgaaaaa aataaacttt atgaataata tcataacgtt tgttcgaatt
    20521 ttatttgtat ctttagtaat aatatgcttt ggaatatatt tatattggaa aaaatgttca
    20581 aaaactaagt aaagagagga ggttagattg tgttattttc aagtttaatt tttttatttt
    20641 actttttacc tataacattg gttttatatt atgtatttag gtttaatcga acgatacaga
    20701 acatgatatt gcttgctgtt agcttatttt tttatgcttg gggagagcca aaatttgttg
    20761 ttatcatgat agtttccata ataatgaact atatatttgg tttactggta gatagatata
    20821 gggaaagtaa aataaaagta aaaatttttt tagttttgat gtgtgtatat aatataggtg
    20881 ttttatttat atttaaatat ctagcttttg cgctaagaaa tattagtgaa cttgttaata
    20941 ttgaactaac tatacctaat attattttac ctataggaat ttcatttttt acatttcaag
    21001 gaatgtctta tgttatagat gtatatcgta gacatggaga agtccaaaag aatccttttt
    21061 atgtgggact gtatatagca tttttcccac aattaatagc aggtcctata gttagatatg
    21121 agagtgtggc agaacaaatt ctcaatagaa aagaaacttg gaataaattt tctattggga
    21181 cttgtaggtt tataacaggt ctaggtaaaa aagtattaat ttcaaataac atggctatag
    21241 tagcagacta tatttacact atgaatagtc aaggagaaat agctgcatca cttgcatggc
    21301 ttggttcaat agcctatact atgcaaatat tctttgattt ttctgcttat tcagatatgg
    21361 ctataggctt aggtcttatg tttggtttta aatttgaaga gaactttaat tatccatata
    21421 tatcaaaatc aattagtgag ttttggagaa gatggcacat ttctttggga atgtggttta
    21481 agagctatat atactttccg cttggaggtt ctagagtagc aaataaagat attatgatta
    21541 gaaatatgtt tatagtgtgg ttatttactg gaatatggca tggagcagaa tggacatttg
    21601 taatttgggg gattttaaac tttgttttcc taattataga gcgatttata atgtttgaaa
    21661 agattgaaaa tcacaatttt ataaaacata tttatgcttt attagtagtt aattttgggt
    21721 gggtattatt taggtcacct aatttaaaag aagcatataa ttattttaaa gctatgcttg
    21781 gaggcaatgg ggttatatgg agtgattata catatatgtt tttaaaagaa tactgggttt
    21841 tctttatatt tgcatttatt tttagtgtgc ctattgcaaa gaaaatcaat aagtttgttg
    21901 ttgaacaagt taaatatagt atgatattta atgtatttta tccaatctca gctattttat
    21961 tgttctttat ttctgtgaca tatttagtaa ctggaagcta taatccattt atttatttta
    22021 atttttagct gaggaggaaa agtatgaata gattttttaa agtaagatta ataactgcaa
    22081 ttatgttctt aattgtttta ttcgctttct ctatatttaa tatttgttat tctggaaaag
    22141 atatgataca ggagataaaa aatttagttg ctgaaaaaag tagtttaaaa gatacaatat
    22201 catctattga taacacagta aatgacaaag taatatataa aaatacgttc atagaaactt
    22261 atggctactt acaaaagtta atgggtaaaa atgagttttc aaaatttagt tctgtaaaag
    22321 atacttctgg aaagatgcat tatacatatt ttgctaaagc tccaaatcca attggtgtta
    22381 tatcagatag agtaagaaaa ttttctgaag aaatggaaaa gcaaaacact aaattggttt
    22441 acctaatgac tccagataag tatatacaag gagtgacaaa atttccaaaa gggatacctt
    22501 attcctatag caatgagaca gcagataatt ttttaaatca actaaaaaag tataatgtgg
    22561 actatattga ttttagagaa aatattttaa agagtggtat tccaaaagat gatttatttt
    22621 ttaaaacaga ccatcactgg aaagtagaaa cttctttttg ggcatttggg gaattagttg
    22681 agcaacttaa taaaaaatac aatatgaatt tagatgaaaa tcattattat agagataaag
    22741 aaaattataa ttcaatttta tatcctaagt catttcttgg ttctatggga agaaagtctg
    22801 gtattttata tggtggtata gatgatttta cattaattta tcctaaattt aagactaatt
    22861 atacttatta tacagattct aaatttcaaa aatttgaatt aaaaggaagg tttgaagaga
    22921 gtttggtatt atcatatccc tttaatgcag accttaattt aatggatggt caatcagata
    22981 aatattctac atatttattg gggaatagac cactagttaa aataaaaaat atagaaaatc
    23041 cagatggatt aaaagtgcta tttgttaagg attctctaat tgtaccgaca gcagcatttt
    23101 tttcctctgt ttgttcatct atagatatga tagaccctag gtattatgat ggagatatac
    23161 tagaatatgc aaaatctcat gactatgact ttgtttttat atctatatat ccccaaaatc
    23221 ttacaaaaga attttttccg ttttgtgagt aaatttctaa agaaattttc ttataaaata
    23281 aaaaacaaat ggctaactgg ttaaaggtta gccatttatt ttaagagtta ttctaagaaa
    23341 tatttttttt actaaatact tctttatcta tttttccaag tgattttcct acagtaattg
    23401 atgataccat agcaccatta acatttatca tagtacgtcc catatctaat atagggtcta
    23461 ttgcaagtat accaccaact aatggaaaat aagaaccaag accaactcca gagataacaa
    23521 ctgaaacggc cattgtagca gttcctggaa gaccagctat tccaaacgaa ctaattgtta
    23581 taacaattaa caacataaca taaaagctca tatccatgtt tactccagcc atattggcaa
    23641 gtgttacagc cattaaagca ggatatatac ctgcacaacc attcataccc atatttgcac
    23701 ctaaacttgc tgtaaagctt gatacaccct catcaactcc gtgattttgt actagacttt
    23761 ctatcgttac aggcaatgtt cctaaactag aacgtgaagt aaatgccaga agtaaagctt
    23821 cagttgaatt ttttatataa gtaatgggat ttagaccatt taaagttata aaaattaaat
    23881 gtatgataaa taatattgca actgatatat aaagagctaa tataaactgt aaaacagata
    23941 ctattgaact aataccttgt gaagttattg tatttgcaag caaagcaacc acagcataag
    24001 gcataaattt aataacagtc atagacacac ttacaattat tttgtaagat gcttctataa
    24061 aatctataaa tggttttatt gtttcaaaat gttttttact taatctcttt attgaggtag
    24121 ctataaacgt agcaaatatt attgtagcta caatatttcc atttgccata gccattacag
    24181 gattagatgg tagtaatcct cttaaagtat ccacaagaga gtttatttct ttaagttctt
    24241 gattttcaac taaagtaggc atattagctc ctacacctaa cttcattata tttgcaacga
    24301 taattccaac aattgcagct atagcagtag tagcaagaag catacctaaa gatttaaatg
    24361 taagagttcc aagattgtta tctttcatat ttataattac ttttataatg gataagaaaa
    24421 ctaaaggtac tactaacatt tttaataagt ccataaatcc agaaccaaat aggctatacc
    24481 acttgcttac ttcttgtagc caagtgactt gagtggtatc attaggaaaa ccagcaaaag
    24541 cttgtattat gactcccaat ataagtccta gtactgtagc atatattgtt ctcaatgaaa
    24601 attttatttt tttcttttca aattgtctaa caataaagaa tgtacctaga agtaatatta
    24661 tgaaaaaaat cgttttaaaa tctgtaatca taagaaattg tgataaaaat gaactttcca
    24721 atgttttttc tccttttatt taaaaataaa tttttctaag tttaaatgat atcaatatat
    24781 taaaaaaatg tcaatgttat atcacaattt acttaaatag agtaaaacaa gttgattttg
    24841 aaaaatgtga ttaagtatag aaaaataggg tataataaat gtttaaagaa tttgaattgt
    24901 ggtaagaaaa taaaaaatag cttttataag atttgatttc aaattaaaat atgttaaata
    24961 aataactaaa aattgttatg aaaaatgttt acatagaata tatatcaaat gaaaagctta
    25021 aaataagtga agaaattaac tagacatcaa aggagagata aaatgagagt agtaataatt
    25081 ggtggtgttg cagctggtat gagtgctgca gcaaaattaa aaagaataaa acctgagtat
    25141 gaagttgttg tttatgaaaa aactgaaata gtatcttttg gtgcttgtgg gttgccatac
    25201 tttgttggtg gattttttga tgatgctgat gaattattag caagaacacc agagaaactt
    25261 agagaagctg gaattgattt aaatatattt agagaagtcg ttgaggttga tagtgaatct
    25321 aagaaaataa aagtaaaaaa tataaaaaca gatgaaatat atgaagatta ctatgataaa
    25381 ttgatgatag caactggagc aagaagtatt atgcctccta taaaaaacat aaaattgaag
    25441 aatgtttcta cattaaaaag tttatatgat ggagaatatc taaaaaaact tttaagtaat
    25501 gaagataata aaagagtgac aataatagga gctggtttta tagggttaga agcagttgag
    25561 gcatgtaaaa aattaggtaa agatgtagat gtaattcagt tagaagatag aatattgcca
    25621 caagtatttg ataaggagat tacggatgta ttagaagaag aaataaatag acataatgtt
    25681 aatttgcact tggatgaaat ggttgtagaa ctttatggag aagataaagt agaaaaagtt
    25741 ataacaaaca aaggggaaat agatacagat gtagtaataa ttgctactgg tgtgagacct
    25801 aataccgagt ttttgtcaaa tactaatata aaaatgctaa aaaatggagc tatagtagtt
    25861 gatgagtatg gaagaacttc tgtagaggat atatactctg ctggagattg tgcaactata
    25921 aagaatatag taagtaatga aaatgtatat gttcctttag ctactggagc taataaatta
    25981 ggtagaatag taggagaaaa cctagcagga agagaagtat cttatcaagg ttcactatct
    26041 tcaagttgta taaaaattat ggatatggag gcagcatcaa ctggaattac agaaagacaa
    26101 gctaaagatt taggcataaa tatcaaatct aaatttattt ctgattataa tcaaactcat
    26161 tattatccag gaagaaataa aatatatgta aagcttattt atgatgcaga tacaaaagtt
    26221 attttaggtg gacaagtggc tgggtttaag gatgctgtcc aaagagcaaa tgtattggca
    26281 gctgctatct ttggaaaaat gaccacaagt caacttggaa tgttagattt atgttatgct
    26341 ccaccatttg caagaacatg ggatgtttta aatgtagctg gaaatgtatc taaataatat
    26401 ttagattata gctaatatag tttacaagca attaagggaa tgaatttacc taaaatattc
    26461 ttatcatggt aaaattcatt cctaaatttt tattccaaat ttttagaaat aaggattcta
    26521 ttaaattatt catcaaaatt taaacgcaag cttaatctat cctctccaag aatagtattt
    26581 tcaaaaacat tagtagcatt ttctaaatac atactaggta tgtctaaaga aggactaaaa
    26641 tgagtcagta aaagttgttt gacatttcca agttttgcaa gattagcagc ttctctaaaa
    26701 gtcatatgtt tgtttctaac agcttttgat atatctaaat catcaccata catggattca
    26761 catataaata aatcactatc ttttatgaaa tctggtattg attctatagg tcttgtatcg
    26821 gttgtaagac ttatcttaac tccctttctc aattctccta agaccatatc tggagtgtat
    26881 tgttttgaat ctaaaactat attttgacct tcttggagtt tgttccataa tattttagga
    26941 acttcgtttt tagttgcctt atctatatca aatttagggt ttcttctaaa attaaaactg
    27001 tatcctaaac actctataga gtgttctaaa ctgattgttg aaatttctaa atttcttaaa
    27061 actttattat ctaaagaaaa attgccttga gggttttcta taatttttaa agtgtatggc
    27121 acatattcta ctaaatttct catagaacgt acacaatcga ctattccaac tggaccaact
    27181 atagttaaat cacttgtacg accactattt ccaattgttg ataaaagtcc taaaagtccg
    27241 aatatatggt caccgtgtaa atgggttata caaatcaagt ctatatcttt aaagccacat
    27301 ttttttagct tcatagatac ctgagtacct tcaccacagt caattagtat ttttctacct
    27361 ttatagttta taaatacaga ggatagaaat ctgtttggca taggtacatt tccaccacat
    27421 ccaagtaaag ttaaatctat cattttttcc cctttctaaa attaacacaa atctgttgtt
    27481 tgtttttatt atgatatcct attataatta ttttaacatt aaatataaca ataaatgtgt
    27541 atttgagtca agaaatattg tcaaataaag tttttatata acaatatata aaaatttgga
    27601 gaacgaaaag tgaaaattat atttaggagt atttaaagga ggaaaattta atgaaagtat
    27661 taaagaaaat tactattttg tcattgatta cattaacttt agttggttgt agtaacggag
    27721 aagagaaaaa agtggttgaa ggttctaaga aaactcaaaa ttctaaaaaa gatgaacagc
    27781 ctataatagt acataaagat gcaaatatat tgaaagatag gtatgagcca ccaaaaggat
    27841 ttaaaagagt tgatgtagaa aaaggcagtt ttgaatcatt tcttaggaac actaagctaa
    27901 aaaattatgg agagaaagta aagtattatg atggaagaac taaggaatca aaaggtattt
    27961 atgatagtgt atttgatgtt gatattggca atagagattt gcatcaatgt gcagatgcaa
    28021 ttatgcttat gagagcagaa tacttgtatc agaataaact atatgatgaa ataagttttg
    28081 attttgttga tggcttcaag gcgaagtatt caaaatgggc acaaggatat agaatttcag
    28141 taaaagatag tggttcaagt tggtataaag caactgaata ttcaacatct tatgagagtt
    28201 tcagaaagtt tatggatata gtgtttgcat attcagggac gttatctctt gaaaaagaat
    28261 tagagcctgt aaaagttgag gatatgaaaa taggagatgt gtttatagtt ggaggaagtc
    28321 caggacatgc aagtataatt atggatatgg cagaaaatga aaagactggt gaaaaggtat
    28381 ttatgatagc ccaaagctat atgcctgcac aacaaacgca acttcttatt aatcgtaaaa
    28441 atccaaattt gagtccttgg tatagtttag attttgaagg tgatttgaca atacctcaat
    28501 ggacttttaa aagagaccag ttgaaaagat tctaaaataa taattttata taataaaaga
    28561 aaaatataga tgtgaaaaaa tgtaattaga ttaaaactaa ttggatatgg acaaaattaa
    28621 attgatagat aaatattaaa aattacacta attaatctta ctatagttta atatttatct
    28681 aaaaagtttt atgtaaaata taaattttat attaaagcta aaactaattt gaggaggcta
    28741 atttatattg aagattagaa ggtatttaat tttggtaatt ataattctaa ttacaacaag
    28801 tcagagtttt atttcttatg ggctagatcg atttgaagaa ataactattt ttcacactaa
    28861 tgatgtacat ggaagatacg ctgaaggtga tgaccatata caaatgggaa atctagccac
    28921 attgaaaaaa gaaactccaa actcaatttt agtagatgca ggagactgtt tgcatggact
    28981 tccaatagta aatatggata gagggaaaag tgcaattgaa ttaattaagt gtgctggata
    29041 tgactatatt actcctggaa atcatgactt taattatgga aaagatagat tgtttgagct
    29101 atctaaaagt gctaatttag gagagaatac tttaaaattt ttatcatcaa atatttttga
    29161 aaatgggaaa aaagtttttg atagtaatga tataaaagaa atagatggaa taaaaatagg
    29221 attttttgga ctctctaccc aggaaacaaa gagcaaaact ggatttaaaa atgtggaagg
    29281 gttagagttt aaaaatccta tagaaagtgc aacagagcaa gtaagtgaat taaaaggaaa
    29341 aggtgccaat gtaatagttg caatatgtca tattggtaca aacagtttaa gtaagccaaa
    29401 tagtatagat atagcaaatg aggtccaagg aattgatttg attattgatg gacacagcca
    29461 cactaagttt gaaaatggta aacaagtggg tgatacattg attgtaagtg caggacaata
    29521 tttagaaaat attggcgaga taaaactaac tttggatact aatgatgtag aaaagataaa
    29581 aatagaaaat aaatcagcta gattaattgg aaaagaagaa gcattaaagt ataagcttga
    29641 ctccgttgta agtaaaaagg ttgatgaaat aaaagaacaa caagaaaaaa tattagataa
    29701 agttataggt gtaactacaa atactctaga tggttcatat gaaaatgtaa ggacaaaaga
    29761 aactaattta ggtaatttaa taagtgacat tttactagat aaaactaagg ctgatatttc
    29821 tttatttaat ggagggaata ttcgagatac gatagaaaaa ggcaatataa caagaagaga
    29881 tattgtagat gtctttcctt ttagcaatac tatagttacc aaagagctta caggagcaca
    29941 gattaaaaat gtattggagc atggagtaaa actttatcca gaaaaaagtt cagccttttt
    30001 gcaagtagga ggaatttcat attactttaa tcctaaacaa aaagaaggtg aaagaataac
    30061 aagtattcaa aaagaaggaa agccattaga tttaaataaa aagtatgtag ttgcaacgaa
    30121 tgattatata gcctcaggtg gagatgaatt tccttgtttt tcaaaagagc cagtactaaa
    30181 ggagtttgga aatttagaga gtacagtcat tgaatatata gagtacaaaa aagaaatatc
    30241 aaaaaatgta gatggaagaa ttggtattaa atctaaagat gagttaataa gaaagaatca
    30301 tgagagtgat ttgatattaa gtgaaaatga aaaaaatatc aatgataatg aagagaatct
    30361 atctaataat ataaaaagct ctgatagtga taaaaataaa agcttagata aaacaagtgc
    30421 tagagaaagt tctaatctag gaatatctaa aagagaagct aaagaaaaat ctcctaaaac
    30481 aggagattta ggtttttcaa atagtataat tatattttta gtttcaagta cattaatatg
    30541 tttattgaat tttaatcaaa aagagttaaa ggataaaaag tcaaaataaa aattaatgag
    30601 caaaaattag tagatagtaa tttaatttta gataatagca aacagcaaag gaaattagtt
    30661 atataaatac taatttcctt tgttttatac tttaacatga ttacttttaa ataaaattgt
    30721 attttataaa aaataattac actaaatggt atagtattat gggtattttt agtttttatt
    30781 caagtaaatg aaatcatatt aacaacttct gatatattta caaattgata aaaaaatgat
    30841 tttattagta tgatataatt tagctagtat gtatgataat ggaggatata gggatgaata
    30901 atatattact tttggaagat gacaaatctt taaatagagg aattagtttt aaattaaaaa
    30961 aagaaggata caatgttttt agtgcatttg gtatagaaga agcaaaaaat attttcataa
    31021 aagaagagat atgcttaatt atttccgata taggtctacc tgatggtagt ggttttgact
    31081 tttgtgagga gattagaaag aaaagtgatg tttacataat aatgttgaca gctcttgatg
    31141 aagaggtgga tattgtcaca ggatatgatt taggcgcaga tgattatatc acaaagcctt
    31201 ttagtttgat ggtattaata tcaaaagtga atgcgcttat gaaaagggta aatactgtaa
    31261 aaaattatac tctattggtt tgtgatgatt tattttttca ctatatagaa aataaattga
    31321 ttataaaaac agatgataaa gaagaagaga taattttaag taaaacagag actaaactct
    31381 taaaatattt gatggaaaat tctatgcaga cattgactaa agagcaactg ttagaatctt
    31441 tatgggataa tagtggtaat tttgtagatg ataatacgat tgcagtaaat ataaggagac
    31501 ttagacaaaa agttgaaaaa aatccatctg aacctaagta tataaaaaca gtaagagggg
    31561 ttggatatat gtggggagaa aggagtataa aaaaatgcta aaaaatatga acctcaaaga
    31621 tggtttaagt gtgaaagatg attatataat gagaagagtt attggatatt ctattttatc
    31681 tgtactaata gtaacccttg tatttatact ttttgaatat agtagattac ataaattgta
    31741 tgaatatcaa ataaaaaata ctcaaaatat tgctggaata ttaattgaaa aatatcctaa
    31801 tgatgaaata gatatagtaa aatctattta taattctgat tatagtaagg tgtctattgg
    31861 agaaaaggca tttaagaaat ttggctatgg attggaaaat aaaatgtcaa atgataaaaa
    31921 ttttagttta tacttaaaat ctttttttaa ggaatcattt tttatttttt taatgatgtt
    31981 tatagtagtt atagatataa tatattattt tataagatac ataaataaaa gattgagtaa
    32041 aatttatttt atagtagata agatggcaca agaaaactac ttgaaggaca ctactgagtc
    32101 agaagagact agttatctag tagaatataa tgagtatttt aaagaaggta catttagtaa
    32161 gataaataac tgtctatatg agcttaatag aagtttaaag ataaagttta ttaaattaga
    32221 aaaagaaaaa gaaagtgtaa aatcattagt aacagatatt tcacatcaat taaaaactcc
    32281 tttagcatcc ttaaaattat acaatacatt acttatagaa gaagaattag aagaagatga
    32341 agccaatgag ttcttattaa ccaataaggc ttctataaat aaattagaaa atttaataaa
    32401 ttctttagtg aacatatcaa ggttagaaat tagcatgata aacataaaaa aagaagataa
    32461 tgatataaaa aatactattc taaatgcagt aaaaagtgtt acccctaagg ctaagagcaa
    32521 aagtataaca ttaaatttaa atgaatttaa cagtagaatt ataccacatg ataaaaagtg
    32581 gactgaggag agtattttta atgtattaga caacgctgta aagtacacac agataaatgg
    32641 agaaataaat atatctgtag aagaaactgc taattacttt aaaattataa tagaggataa
    32701 tggcatagga attgaaaaaa atgagtttaa taatatattt aaacgttttt atagaggttc
    32761 tgctgatgaa attgaatctg tagaaggtag tggggtagga ttgtatttaa gtagaagaat
    32821 tttagaagag caaggtggaa atattatagt ttcttcaaaa gtagggatag ggagcaagtt
    32881 ttctttgttt cttacaacta tgtaatatta aatgaaagga aaatgtaagc ctctacattt
    32941 attatataag tataaacagc tccagaaata aaaaattaat aaaatatggg gtaatataac
    33001 ttagaatgag aggataattt tatgaaaata ctatatactg aaaatttatc aaagcattat
    33061 ggaaaaggag aaagcttagt aaaagctttg gataatgtta acttagaaat aaatgaaggt
    33121 gaatttgttg caataatagg aaaatcaggt tctggaaaaa gtacactttt gcatatgatt
    33181 ggaggtcttg atatacctac atctggcaag gtgtatatag ataataaaaa tatatttaca
    33241 ttaaaagaag aagaacttgc tgtgtttaga agaagaaaaa tagggtttat atttcaaagc
    33301 tataatttaa ttccatctct taatgtatgg gaaaatgtag tgttgccaat aggtctagat
    33361 ggtagagagg tagatgaatc ttttataaaa gaacttttga aatcattagg gcttgaaaat
    33421 aaacacgacg ttttaccaaa tacattatca ggtggtcaac aacaaagggt ggcaatagca
    33481 agagcattgg ctacaagacc agctataata cttgctgatg aaccaactgg aaacctagat
    33541 tctaaaacat ctgatgaggt tatgagtata ttaaaatcta tgtctaagaa gtacagtcaa
    33601 actctagtta tgataactca tgatgacagt atagcccaaa tggctgatag agttatattc
    33661 atagaagatg gcagggtgtc aaaggttggt gataaaaatg attaatacta ctaaaatagc
    33721 aaaaagtaat ttaaaacaaa ataaatcaaa aagtattcta ataataatta ctataatact
    33781 ttcaacctta atgctttcat ctattggaat ttatattgtt gatgcagggg catatcaaaa
    33841 agaaaatact ataaagtatt caggcaatta tcaaggtata ttagcaaatg tagacgaaaa
    33901 acaagctgat atattgagta atcatgcaga tgttgaatta actggtaaaa tgaatggagt
    33961 aggtgttgaa aaactagaag atgattcaaa tatttcttta gcatacatga ataaagatgc
    34021 tttgaaatta aatagttttg aatttgtcaa aggaaaattg ccaataagag agaatgaaat
    34081 tgtattggat agtggggctt taaaagcact tggttataaa aataaagatt taggtgaaaa
    34141 gataaaaata tcttataatg actataaaaa tgataaaaaa attgaaaaag agttcattat
    34201 aagtggaatt ttaaaaacta gcgaaatatc tgaagcaggt aaatattatt atgctattat
    34261 atcagaaaca tatatgagaa atactagaga tatgtcacaa gaagatttta atatatatgt
    34321 gaaatttaat gataaaagta atttatcaat agaacaagta aaagaaaaat taaataagat
    34381 tgctaatgat ataggactag atactattaa tactgctgta aatgaaaatt atataaatgc
    34441 tttaaaacca gatatggaaa cgattatggg tggagttttt gtaggattag taattgtatt
    34501 atctagtatt ttagtaatat acaatatatt ttatatttca atagttacga aagttcaaga
    34561 atttggaaaa cttagagcaa ttggtgcaac taagaaacaa ataaaaaata tagtgtttaa
    34621 agaagggttt atattagccg gaatagcaat accaatagga ataattttag gttatgtact
    34681 tgcaaatatt ataataaaat tatttatgga tatagatgta aaatcatcac gattaccagt
    34741 tatattatcg gttgtggtaa taagctttat atctgttgtt ttatcacttt taaaaccaat
    34801 gaaagtagca tcaaaagtat ctattgttga tgcagtaaga tatacaggaa ataaaataag
    34861 taataagaat aagagaaaag gatataaaaa tataaatcta aagagattat cacatgccaa
    34921 tttagaaaga aataaaaaac gtacttacat gacattggca tcacttatac ttagtggaac
    34981 tatatttata acagtatcaa ctgctttaga aagttttgat gctgagaaaa tggcaagaga
    35041 acactttcct tatgatatag aagttagact atctggttat gaaatgaaca gtgataaaaa
    35101 tcctaaaaat aatttgaata ttttacaaat ggataatcct ctaagtaaag atttttttaa
    35161 tcaaataaag aacatggaag gaattaaaag aatagaatct gcaagaagtg taaaaattgg
    35221 tatggaggac tatgatgttg aatttaagta tgacctttta caaagtataa atgaaaatga
    35281 tgtaaaatca ttaaacaaga atctaataga tggaaaaata aatcttgaaa gactgcaaac
    35341 tggagatgaa atagtaatta ctcatgttga tacagcaaaa gaaatgggag taaaagcagg
    35401 agataaaata agattaacct tatatgatgg agataaaaag ataaaaaaag aatttaaagt
    35461 tcaagcaata gctatggggg ttccaagttt tggtataggg aaagatttta tagatagaac
    35521 tttgaaatac gattcaacta gtagcttggg aatatataca gaagaaggga agtatcaaga
    35581 aatcaaagat agtatcaaga agattgctaa aaataatgga tttttagaga ctgattttat
    35641 agatagtaga attgagtcaa ataaggctac tatttcattt ataaagataa tgggatacac
    35701 gctaacaggc ataataggtg tgattggatt tatgaatctt gtcaacacaa tgataactag
    35761 catagttact agaaaaaaag aacttggcat gcttcaagct attggtctta ctaataaaca
    35821 attagtgaaa atgcttaatt cagaggcagt gtattacact tctggaatga tgataggaag
    35881 tattttattt ggaggtatat tagggtatat tgctgttata cttctaaaaa agactggatt
    35941 atcttatgca acatattctt tgccaatagt accaatcttg ttgatgattg tgtgtattct
    36001 tatagctcaa tttataacaa cttatttgat aggacgaagt tttaataaag aatctcttat
    36061 tgatagagta agatacagtg aataaaatgc aaatatttat ggttagttta actagcaaaa
    36121 agcaaataaa aaatatagtt aaagagattc ataattaaat aggttaaagt atagtcacag
    36181 agtaaacaat ttaaactgat gtttgctctg tattttatta tgtaatcaat aatatatcta
    36241 atagactgat attattcatg ttggaaaatg tatcggtaca attctaaaaa agtataaaat
    36301 tgtaccatat aaaatgaaaa ttttgtataa taatcgtata gaaaataaat aggagctgat
    36361 atatatgaat aagaagatta attatgcacc aggaccaact gaaactaggg aaaatgtaag
    36421 attagcaaga gcagaaaaaa caacaaatcc agatatagat attgattttg ttgaatttta
    36481 taaaaagaca tgcgagaagt ttggcaatat aattggaaca aaaaacgatg tttacatatt
    36541 gagtggtgaa ggaatattag ggcttgaagc agcatgtgca tcacttacag aaaagggaga
    36601 tagagtactt gtaattgaca atggtatata tggagaaggt tttaaagatt ttgtaacaat
    36661 gtatggtggg gagtatgtgc ttttttcaag tgaatatact aaaagtatag atattgatga
    36721 attgaaagca tttttagata aggatagtaa ttttaaatat gcaacagtag tgcattgtga
    36781 tactccaact ggtgtattaa atgatgtaag caaaatatgt ccattactta aggaatatgg
    36841 aatattaact gtagttgatt ctgtagcggg tatggtagga gaacgattaa gtgtagatga
    36901 atctaaaata gatataatat taggtggttc acaaaaagct atttctgcac cagcaggact
    36961 tactatagtt ggaataagtc aagatgcaaa aaattgtata aaaaatagaa aaactgatgt
    37021 aataggtttt tattgtaatt taaatatatg ggaaggctat tatgaaaaaa aatacttccc
    37081 ttacacaatg ccaataagtg atataatggg gcttgataaa gcacttgata atatattaga
    37141 agaaggaata gaaaatgtat taaatagaca tgaaaagata gcttatagtg ttagaaaatc
    37201 tattgaggaa tatggtctag aactattttt agaagaagga tattctaata cagtcacagc
    37261 tataaaagtt cctgaaagta taggagcttt aaaacttact gattatatgc ttaaaaatta
    37321 taatacactt gttgcgacat cactaaatca atatatggat actattttaa gaataggtca
    37381 tatgggagaa aatgcaaatc taaataaaat agagcacata ctaaatgttt tggataaaag
    37441 tttaagtgcg ttaggattta aaggaaatgg aaatttgtta aacttattta ataagtatta
    37501 tttttaatag atagataggg ggatatagta tggaagttac agctaagcaa aaatttacaa
    37561 cgaaagacat ggtggaaacc tcactattaa tagcattagt atttattgca actaaattta
    37621 taaatataag attaccaata tctataaatg gtgggcttgt gcatttagga actgcaatgt
    37681 tattcatatc agcaatagta tttggaagta aaaaaggtgc tttatcagga gctattggta
    37741 tgtcactttt tgatttaata tctgggtgga ctttatgggc accatttacc tttatagtta
    37801 gaggtgttat gggttattta cttggaaaga ttgtatgggc gaatggtaat aatgggaata
    37861 attttttaat caatgtaata ggaatatgtg tttcatcaat ttggatgtta tttggatatt
    37921 atgtaacaga agttatactt tatggtaatt ttgtagtacc tcttacttca ataccaggta
    37981 acttaatgca agtattgata ggtcttataa tagcgcttcc tatttctaag gtattaagaa
    38041 aatgtataaa ataaatataa agtgaactaa aatttacgat agcataaatt atactgtata
    38101 atattaagat ggagtacata attaaaaaat tttagtatag aggttagttc acaatgttat
    38161 cagttacaag atattcaatc aaaaatattc gtttaaagcc gtgggtgaag tttatttggt
    38221 attttgagac aaaatcaaat atactgttaa ataataagtt acttccgaca gacagtatag
    38281 atattattct taatctttct gatgttatgg aatataaaat tgaaaatcaa gactatactg
    38341 ctagtaatat gcattttaat ggaataaggg ataaacatgg atttattatt caatatggaa
    38401 atatacgtgt tatagggatt tctttttatc catttggtct atatccattt ttaaagatac
    38461 cgatttcaga gtttaacaga caaattgttg acctagaagc aatatcaaaa ctatttgcaa
    38521 aaaaattaga agagtcatta aatccaacaa aagcaataga aaaagttgtt ttatgtttag
    38581 aagaagtatt attatccata ttagaagaag atttaatttc aaataaatct gtagagcttt
    38641 taaattcatt tatatatgtt aatagatata gcaatataaa agcattttgt gatgatacaa
    38701 atataaatat taaaacattg gaaagaacct gtttaaaata tacagggtat acacctaaga
    38761 ttttaaagag aatttacaga tttaaaatgg caagtaatca acttatttat aattataaag
    38821 atgatgaact ttttgatttt atttatgaaa atgagtacta tgaccaagca cactttataa
    38881 aagaatttaa aaaattttca gggacatcac caattaaatt tattggtgag aataagacga
    38941 ttaaagaaaa tataaagtac agctatttat aattatactg tcgatttttt acaatacctc
    39001 caatttattt tttgatatga tataggaaaa taaattggag gttttacata tgaaaattat
    39061 tacagaaata gttgagttta atgtggaaga aaatttgtca aaagaagagt ttatagaaat
    39121 tgtaaatgac ttagaattaa aatttcattc tattcaatcc ggttttattg atactgagct
    39181 tttatatgat gaaaagtctc aaaaatggat tatgatacaa cattgggaat ctatagaaga
    39241 cctcaagagt gcttctagta aaatgtttaa agattcttca actgagaaat ttagaaaagc
    39301 tttaaagaat caaactgtta agatgaatat aataccacaa attaaaagtt ggaaattata
    39361 gaactgaatg taaaagatag ttaaatagta ttaaactaaa ttagccaaat aacattttgt
    39421 atttaaattg ttatttggct tttagtgtga gcgtggatta tcataatgtc atattcggga
    39481 gctaaaaggc ttaatactga tttgccaatt ggtcaatcca tgcatgtcca tctggatatg
    39541 ctgttattaa agcctttaca agccaatccc tttgctcttg tgataatagt gggactgtta
    39601 catcgaagtc tttttgataa ccaactctat ctatgtatat tgatttgcaa aacaaaacca
    39661 cttcgggagc catatatgga atattatatg atgttatcag tctggcctta tccatatctc
    39721 ttataattct ttgattttgg gaaaaaagaa attttccatc tttctgtgga ttatatatca
    39781 attcaataaa atcgaactgt aactgttcat cacactgtat attcatttta tagttaccat
    39841 cctcgacttt ttttaattca ataaatgagc aatcgggttt aattgcgaaa gcacaaattt
    39901 gctctatatg ttgcttgtta aggtcgagaa taggtcgtaa taaataatta tccaatggct
    39961 catatatatc ccatccttga ttgagcatat actgaatgct cttttcttta tctgtgtcag
    40021 aaatccatat gtcaatatcg ctgtgtgggc gaatatgcat attcgcaaat aattctaggg
    40081 catatccacc acagattgaa tatgaaaagc cacagtcatt aaaaaaattt acacaatctt
    40141 taattagttt gttcataata gcctcctttt gcatctactg aataaatcaa atttttaaaa
    40201 ataccaacta aatgtttgtt ttgtgtaaat ataaaactgc cgatattaag gcagttttat
    40261 atttactatt tagaaaattt attaacaaat ttttgatatt cacacattat cttatcattc
    40321 ataaaagaaa tacccataaa agcagcttgc tcaccaaaca aagtaaaacg ttctatcatc
    40381 tgttgtggat tacctggata aactgttgga atacaccatg tgtttagaag aaaaccaaac
    40441 atttgagcag caatctctgg atttttaact tgcatagaat tatcttgatt tccttcttca
    40501 atcaatgctt ttatccaagg cacaatatca tttataatct catcaaactg cgtagctaaa
    40561 tacttaggat tacgcaatag tgatgatgac attaaaaaaa tgtcttgttt attgtgttgt
    40621 tcataagcat gaagcaacct atattgtaat ttttctaatg cggtctttcc ttcatcattt
    40681 tttacatcat atccataaaa tccatcatta tacaaacgac ttgtgattgc cttaattatt
    40741 tcatcttttg atttaaagtg atgataaata gcacctcttg aaaggccatc taaattatct
    40801 acaatatctt gaatcgttgt ctgctcatac cctttttcta agaacaactt atgtgaaata
    40861 tcaagtattt tttgtatagt attatcaggt tctttttttc ttcccaaaaa gctcactcct
    40921 caaaacaatc atttagtatg tttatcttag caaatacata atgatttgtc aatgtgtctt
    40981 tttagcatgt tgaacaggta tattgccatg ctttcattat tttatttttt tgccttatac
    41041 tggactatat ttgcagtttc ctaatatatt tggctcatat ttaaatacca ttatctaaaa
    41101 gcactattgc tgtacgagag gcaataggca ttttagaaga aggcggagtt gagctaaaat
    41161 atacatcata atctcttaag ttttcttttt ttgtgtttcc tccaaactct accatatcag
    41221 tatttaatat cgttttgtag tttgtatttt gaaactttga aatatcaact gattgtatta
    41281 gattagggtg gaaattaaat ataaataaat atttgccctt actgtacacc aaaatctttt
    41341 tatcattatc catatgtata agttcatgac tattttggat aaaaatatca ctgtattttg
    41401 taagttcaag catagattta tcaaaattta ataattgttt atactttaag ttgttatctt
    41461 ctgaaaggct ccattgtctt ctggcatact tatagctcca attgttgcct tctcgtggaa
    41521 aatcaatcca ctcaggatgt ccaaattcat ttcccataaa atttagatat ccttcacctg
    41581 ctagagaaaa agttattaat ttaattaatt taataagaga tattgctcta ttgataacat
    41641 ggttatttga gtttatttcc atattccagt acatttcctt atcagctagc caaaaaataa
    41701 ttgttttatc gccaactaat gcttggtcat gagattcaca atatcctata tttttttctc
    41761 caggtcgtct agtagtaagt tcataccaca ttttgcccaa atcccagtct tcatcagata
    41821 agttagagat agtttttatc caaaaatcag gtactcccat tgcaagtcta tagtcaaatc
    41881 caattccgcc atcttttata gggatacaca taccaggcat tccgctcata tcttctgcga
    41941 tacttatcga attaggattg atttctttta ttaattcatt tgcaaattgg agatatgtta
    42001 tagcttcaat atctgtattc atactaaaat atttttcata actatcaaat gaaactccaa
    42061 gaccatggtt acgatataac attgaagtta caccatcaaa cctaaagcca tcaaaatggt
    42121 actcatttaa ccaaaatttt atatttgaaa gcaaaaaatg tattacttca ggtttaccat
    42181 agttaaaaag ttttgtaccc caagcaggat gattcccctt tgaacctgag tggaaaaatt
    42241 gatgttcact tccatcaaac tcatttattc cttctaaagt attctttaca gcatgagaat
    42301 gaactaaatc taataaaaca gcaatcccca ttgaatgagc agtatttatg agatttttta
    42361 agtcttcagg agtaccaaac ctagatgaga ttgcataaaa gtttgaaact tggtatccaa
    42421 aggatgcata atatggatgt tccataatag ccataagctg aatggtattg tatccagctt
    42481 tttttatctt agaaagtatt ttttcagtaa attcattata agttccaata gattcgcttt
    42541 cagtagacat acctatatga cattcataga ttagtggaga agttatgttt tttaagtcaa
    42601 aatcattgtc agtccagata aatggagttt ttggctgcca tatttgtcca ttaaatccac
    42661 caaaatcttt ttgaattact ctttttatat ataatggtat acggtcaaat gtttttccat
    42721 ttgcagtaac ttgtaccttt acctctgatt tgtgaggtaa gctattttgg ccaggaataa
    42781 atatttccca attaccagaa ccaatttttc ttagaagatg agattttcta ttccaattat
    42841 taaagtcacc aattagtgac aaactatctg catttggagc ccattctcta tagaaccatc
    42901 catcatttgt ctgatgaaat ccatagtaaa gatgagcatt tgcaaaagaa ctaaaatctt
    42961 tatagttttt caatgttttt gatttcatat cctcataatg tttcattcgc ttttttatat
    43021 cagattcaaa tggttgtagg tatacatcaa tatctaaaat tttaaatctt ttttgacttt
    43081 tcattgtatt tctcctccat aataaatatt acatctaaaa aataatattt atctcatact
    43141 atcttctctg tataattgat atatattata taatcctaat aaaaagctta agccaccaat
    43201 gattaatttc ataatttacc tccctagtat ttatacttat atatcgtatt catcaaaatt
    43261 taaaagtttg tttaaaactc ttttaataat atcataatca aagcctttat aggaaagatg
    43321 ttgagaaatt ttttgatata ttttcttttt atcttccttc tttactcttt catacctttt
    43381 ttttgctaga tacatagcat tttcaaattc agtgtcctta tctaattcag atactgcttc
    43441 atcgattgta cttctattga tacctttatt atataaattc tgcttaattc tgtttttgcc
    43501 acatttattt aggttaacat ttgtgttaac aattttttgg gctaatacac tgtcatttac
    43561 gagattataa cttttaagaa aatctatgac cctctcaact atattttcat caaattctga
    43621 tgatagtttt tcttttaatt ttttttcaga ttggtcagca tgagataaaa tatttagtgc
    43681 tttattttta gcttttatat acatttcatc atctaaaata ggttttaatt cttcttcatt
    43741 tatttcattc ccttttttta aattaaatgt ataaacaagt tctttaaata tagcaataaa
    43801 aaatttatca tccacataaa tattaactct atcatcattt cttttttgtt gttctatttt
    43861 tgtaattata cccataagtc ctcctttttt atatgtaatt atattaacaa aagcaactaa
    43921 taaaatttaa caaaaaactt atatttgtat gttatatttt atcaaaatta agacaaaata
    43981 gtaaaaagtt atttttatta aaaagtaaag tagaatcatg ttaaataatt attttttaaa
    44041 gtgtttttta gcatataaat ataattatat acccaaaaaa acaaattata agctttaaga
    44101 aataaaaaat aatggtacaa tagtattata acattaaaag gagacaaaat atgagaagtg
    44161 aaaatctagt taagatggca gagttgaaaa atactgaaaa actagagaaa tttaatcgcc
    44221 ataaaggcaa aatcaaaata ggtatattgg gaggtacttt tgatcctata cactatgctc
    44281 atttagcaac agcagaattt attagagaca agtatgacat tgacaaaata atctttatac
    44341 catcaggaaa tccaccacat aaattatgta tcactacaga taagtatgat agatataata
    44401 tgacacttct agcaactgaa agcaacgaag attttttagt ttcaaaagta gaaatcgaaa
    44461 gaaagaaaag gacttataca atagatacat taaaatactt aaagaaaaaa tacaaaaatg
    44521 cagacattta ttttataaca ggtgcagatg caatttgtag tgttgaagag tggaaagatg
    44581 taaagaaaaa ctttgagtta gctactttta tagctgcaac tagaccagga attagtttgt
    44641 taaggtcaca agagactata gaaaaactaa ctaagaagta taatgctgac attataacag
    44701 tttatgttcc atcactggat atatcgtcca catatataag ggaacagcta aatgaaggca
    44761 agtcaatacg ttacttagta cccgaaaatg tagaaaatta tttatatgaa aataaactat
    44821 atcaatatgg agatgattaa atgaacttag aaaatattaa ccagagacta aatcaaatgc
    44881 taccagtggg gaggcttagc cattcaaaaa atgttgcaaa atgtgctgaa aaattatgtg
    44941 agatttatgg atgtgacaaa gaaaaagctt acttagcagg aatgattcat gattgtgcaa
    45001 agtatttaag tgacaaggaa atagaggatt atgtaaataa gtatgaaata tatttagacc
    45061 caatagagga tggaaatcgt tccttgtctc atagtgtaat tggggcgtac atatgcgagt
    45121 atgagtttga agtagaagat gaagatatta taaatgcaat caaatatcat acaacaggga
    45181 gagaggacat gtctctttta gaaaaaataa tttacatagc tgatttaatt gaagaaggaa
    45241 gaaaattccc agttgtagat acattaagag aattagcata tggagggaag cttgatgaag
    45301 cacttttaac ttcttttaat aatactttaa tgtttgtcat caataaaaaa gaagagatac
    45361 atccaagaac agttatggca agaaactatc taattaaaga aaagttatta taaaaaagct
    45421 atataaaggc aaacatatta ataaatttaa atttgactat gttacaaaat cgtattaata
    45481 tgttacaata actaagtgta ttttaattag gaaagtgagg aatttttata tgacagtgga
    45541 acaaatgaca aaaatagcat atgatgccat agaagataaa ctgggtcaag atacagttat
    45601 aataaatata ggaaaagttt ctagtttgtg tgattatttt attattacta cagcatcttc
    45661 tcagagacag gtaaaagcta ttgctgacaa tgtggaagat gaattagcaa aactaggatt
    45721 agagccaaga ggtaaagaag gacaaggaac tcaaacttgg gtactccttg attatggaga
    45781 cataatggtt catgtattta atgaggaaaa tagagggttt tataatttag aaaaattatg
    45841 gaaagatgca ccttatatag atattgacac attagcataa acatgataat atatgttatt
    45901 ataattagaa aatttcaaaa attatattaa attgaataga ctagagcagt aaaagattat
    45961 attttttcag agagttagtg gaaggtgaga actaatatta tataactttg aacttactag
    46021 attaaaatct attctccatg gctggattaa aagctatcta agagggtcta tgtatatagt
    46081 aggctaatta gggtggtacc gcgaagattt atcctcgtcc ctaaacgtaa gtttagtgac
    46141 gaggattttt tattttcaaa gaattatttg gaaattataa gtaaataata ctaaataagg
    46201 tagttataca caattaaaaa taggaggcaa aatatgagcg tatataattt taaagaagta
    46261 gaatcgaaat ggcaaaaaat ttggaaagat aataatcagt ataaaatgga tactgcacaa
    46321 actgaaaaac ctaattatta cacattagaa atgttccctt atccatctgg aaaaatacac
    46381 atgggacatg ttagaaatta ttctataggt gatgttgtcg caagattcaa aaaaatggaa
    46441 ggctataatg tacttcatcc aatgggatgg gattcttttg gattaccagc tgaaaatgca
    46501 gctataaaac atggaataca tccacataaa tggacaatgg aaaatataga agaaatgaaa
    46561 gagcaattaa atttattagg tattagttat gattgggata aagaagttgc aacttcaact
    46621 ccagaatact atagatttac acaagaaata tttttgaagt ttttagagca tgggcttgca
    46681 tataagaaaa aatcgtatgt taactggtgt ccttcttgtg agactgttct tgcaaatgag
    46741 caagttgttc aaggagcatg tgagagatgt aaagcaacag tattaaagaa agacttagaa
    46801 caatggtatt ttaagactac tgaatttgca gaagaattac ttaatgactt agatacatta
    46861 gatggatggc cagaaaaagt taaaacaatg caaaaaaatt ggataggtaa aagtacagga
    46921 gcagatttag tttttgatat agatggaact gataaatcta tgacagtatt tacaactaga
    46981 ccagacacaa cttatggagt tacttatatg gtacttgctc cagagcatga attagttaaa
    47041 gaattggttg cagggactga atatgaagca gatgttgaag cttttgttaa aaaaatgcat
    47101 accatgacag aaatagaaag aacagcggct gatgtagaaa aagaaggaat gtttataggt
    47161 agatatgtta taaatccttt aaatggcaag aaagttcctt tatggatagc taattatgta
    47221 ttagttgagt atggaacagg tgctataatg gcagttccag cacatgatga aagagataga
    47281 gattttgctg aaaaatataa tttagacata attgatgtaa taacagaaga taacaaaatg
    47341 atagactcag aagagtttaa tggactagat gcttcagaag gatttgaagg aataatagat
    47401 aagttagaaa aagaaggtag aggaaagaga acaattaatt atagattaag agactggtta
    47461 gtttcaagac aaagatactg gggttgtcct atacctgtgg tttattgtga tgaatgtgga
    47521 atagttccag ttaaaaagga agatttacca gttcttttac caacagatgt tgaatttact
    47581 ggaaagggag aatctccact tactacttca aagcaattta tgtcaacaac ttgtcctcac
    47641 tgtggaaagc cagctagaag ggaagttgat actatggata cattcgttga ttcttcttgg
    47701 tatttcttaa gatatgtgga tagcaataat gaaaatgaac catttagtaa ggaattagtt
    47761 aatagatggc atccagtaga ccaatatata ggtggagtag aacatgctat aatgcattta
    47821 ctttatgcaa gatggtttgt aaaggcattt aagagtatgg gaatggtaga cttcaatgag
    47881 ccatttaaaa acttacttac tcaaggaatg gtacttatgg atggttctaa aatgagtaag
    47941 tctaaaggaa atacagtatc tcctatggat ataatagatg agtatggagc agatactgca
    48001 agactttttg tattatttgc ggcaccacca gaaagagatt tagactggtc agagcaaggt
    48061 gttgatggat gctttagatt cttaaatagg gtttatagat tggttgacga gctagcagac
    48121 gtggttaaaa aggatgtaga gtttggagaa ttaaattctc aagataaaga tatgagatat
    48181 actatacact ctacacttaa aaaagttact gctgatttaa gtgaaaaatt cggatttaat
    48241 actgctatat cagctcttat ggaattaata aatgatatgt ataaatataa agagttagat
    48301 aatataaacg aagcagttat aaaagaaggt gtacaaacta tagtaactat aattgcacca
    48361 tttgcacctc atttaggtga agaactatgg actatgatag gtaaagaagg aagtgtattt
    48421 gacatagact ggcctaaata tgacgaaaaa gctttagtaa aagatgaaat agaagttgtt
    48481 gtacaagtta atggaaaagt tagaggaaaa ttaactgtta actcaaatat atctaaagat
    48541 gagatggaaa aggtagcttt agaagatgaa aaaataaaag gattagttga aggtaaaact
    48601 atagttaaag ttgtagctgt acctaaaaaa ttagtcaatg tagttgttaa ataatttaaa
    48661 aatataaaca aaaatataaa tttataaaat aaatgaggct gtctcaaaat agagataagc
    48721 cttttttcat tgttgaaatt ataaaaatgg atttgatttt atacaaaaaa atgattaaag
    48781 aactaaaaag ttcattctta tacatcctaa ttgttgtttt aaacattatt ataattaatg
    48841 ttaaatcgcg ttaatgtatt aagtggtatg taaagcattt agtttttaaa gtattgttta
    48901 tcgaggaact atcctaagct tagatgaaaa aaatgtaatc attgtatttt tgaaattttt
    48961 ttatataatg aaaacaggca aaaaaagcgc atatctatgg ctttctctga aacacccaga
    49021 caacgaaatt cagattaata taatggctac catatttctc aaaaggaact tgcaaaaaag
    49081 ataggcgttt ccacttccca gttgattaaa attatcagca gaaaaacaag gatggtcagc
    49141 agcggtattc taataggtgt tgccgatata ttcaagtttg gattgtctga ggatattatc
    49201 aaagaacttg tgataggtgc agtagatgtg gagattctca accgtctgat ggagcataaa
    49261 aacttccaat gcctgataaa tctgatgcgg atttattctc aggatatggt tgatttggga
    49321 gatatggagc gtaaccagtt tattgatatg gtgactattt ttttgtctaa tttgatgaaa
    49381 gaatccccaa aatactatac agaagtaaga aaggaattaa ctgatggtat aaaatgataa
    49441 atgcaatttt aagaattttg atagtatgat tttaagattt atctgctatt ttgtttttta
    49501 gaaataattg aaatgttata aggaggcatg tacaaatgga gactgtatca aataatcaat
    49561 caggtgtaga aattcaactc agtctgtttg aagctatcaa agatttattg ttaattaatc
    49621 ctattttctt gttgggaatt atcttgattg gtatttcctt aattgtaata ttcaagaaag
    49681 atagtgatat tccaaaggtc aaaacatcta ttttatcact ggcaatgtat tactacttat
    49741 gtttaacact atcgcatatt gttggtatcc ctacattcag tgaatatata agactgtcac
    49801 agttagggga aacgttcttt aatccaaaca taagttttat tccttttagt gatggattta
    49861 gtttgagttt tattttgaat attttacttt ttgtcccatt aggtttttta tgtccgctta
    49921 taagcaaaac ttttgaacgt ttgagaaata catttctaat tggatttggg ttatccctct
    49981 ttatcgaaac tattcagctt tttacgttac acagagcaac cgatataaac gacttaatta
    50041 cgaatgtaat tggaacaata ataggctatt tctgcttcaa actgattgct aaattgcgaa
    50101 ttgtaaaact acattcagat gatgactcta tggaacagga ttatacagta tgtattccaa
    50161 ttgccattat aatagttgcc cttatattgg gattttttag ttaagttcca aatagttaat
    50221 ttgggaatta tggagatcat taagtgaata aaattacttt tatatcttta ggtgtaaaaa
    50281 gtatggagaa gtcaattaaa ttttatagag atggattagg attttagatt acaaacttac
    50341 agtttaccaa ggagatagca aagtcgtgtg acaaagggta accatttgga aatccagaca
    50401 acataacaga tagtatgtta ccttttacag gagcatactg tctgttatct tgtcaacatc
    50461 ctattttatg gtttgtgtgt agcttcttgt aaactgaact aaataattaa atagaagcta
    50521 ttagaattaa taataacaga tagatattaa taagcataaa caaatagtaa aatgtgtaga
    50581 tatagacgtt tttaagtgaa actattataa aataattgat tgacaatcaa ctgacaaaac
    50641 ctattttttt aggtttaact gacatttaac tgacagtcag tttttactat ttaaatagaa
    50701 gttcgcttag attgtcagaa gcttctttgt ccatttcttt aagtacatga gaatatctat
    50761 tcatagttat ttttatatct gtatgaccca atctttcaga aacaactttc atattagttc
    50821 cccctaaaag cagcagagtg gcacttgtat gtcttaaatc atgtattcta atcttttttt
    50881 aattattttt tttgtaaaat tgtgaaacgt ttttcttagt gaagaaggta gaaacggatt
    50941 taaattggtg tttagacaaa ctaagttatt tctgttttcc aggatacctt gcaattttaa
    51001 tttgctttgt cttagttttt caattttcaa tttttcaatt aattcttttg gagctgataa
    51061 agctcttttt gattttggtg tttttggctc tttaaaagat attttgccat tagaatagac
    51121 aagaatttga ttaacattta tcagattgtt atctaagtca acatctgacc acctaagtcc
    51181 agtagcttct tacattctta aacctagaag taacattaaa agaataggta tttctatatt
    51241 attaccaagt aacttttcta ttagcaataa tacttcttct ctattgtata tttcatttgt
    51301 agaatatttt tctttagaag gaagttttat aaaatcacat gaattttctt gtatttctct
    51361 taatctatac acttcactta ttacagaact tagaaagcta taccttactt tagcactagt
    51421 aaaggtagaa ttattaaagt tataatttac aaaagcttgt actgaactag gatttacttc
    51481 tgtgagtttt agattcttaa aaaaaggttc tatgtaattt ttaacccaag attttgtatt
    51541 tgtaacagta tatggagacc aattattttt attcatgtct atatatttat aacatctttc
    51601 tacaaatgtc ataccttttg gagcaacaaa cttattatta tttatagtag actttatttc
    51661 aattaaatgt ttttcagcat cctttttctt ttcatagctt ccataacttt tttgtttctt
    51721 tttgccagtt tcttattcca catatacata gaatttttcc catctttttc ttataaaagc
    51781 tgatttgata ttcatatatg tacgctatct ttataagtta gttttataaa agaacagttg
    51841 gattgctcta attatgtcat tatcttttta attctttaca gttacattat aacacgtatt
    51901 tgtatacact taaattaaca ggaagataaa tatcgttaaa ggaggaaaag aaaatttaat
    51961 gtagaattat taagatatgt agtatttcat atataaaagg tggagatatt gtggaattaa
    52021 agaaattatt atcactaggg ttagccgtgt caatgtttgc tgctggtcct atatcagcta
    52081 atgcacttga tacaatttat actattcaag gaaaagataa atatgaaacg gctgctctta
    52141 tagctgatga acaaaattat actacagcta tattaattaa tacagacagc acaatggctg
    52201 atggattaag tgcgagtgga cttgcaggag caacaaatgc accaattctt ttaactaaga
    52261 aaaataacat acctaatgca actcttaaaa gagtagagaa agctaaaaag gtatacataa
    52321 taggtggaga aagttctatt gataaagcaa cagaaactct tctaaaagat aaaggaatag
    52381 aggctaaaag acttcaaggt agtgatagaa ttaagacaag ctataatgtt gcaaaagaaa
    52441 taaactcaat taacaaagta aataaagtta tattgactaa tgcttttaaa ggagagcctg
    52501 atgcaatgag tgctgcatct gtagcagtta gagataaagc agctatagta ttaactgatg
    52561 gaaaaagtgt tccatttaat acaactggtg tagaaagtta tgtaataggt ggtacttcat
    52621 caatgagtga caaactagtt aatgatacta attcaactag attaggcgga gtagatagat
    52681 atgatactaa taaaaaaata gtaaataagt tctataatgg cgcaaaagaa ttttatataa
    52741 ctagtggtac tgatttagtt tatgctttag ttgcttcacc aatgtctaaa aataatccta
    52801 ttgtgctagt tgatattgga agcaataaag atatacttaa aaatgctgca aaagtaaccg
    52861 ggataggaaa tctaagtgat aaaattatgt tagagtgtga aaataccatc aataatatta
    52921 gtcttccatc ttcaaataaa aatataatat gtggattaaa gattgatttt gattcaaagc
    52981 acataatttg gcaagataaa ggaagttatc ttgaatgtta tgatgataac cttgaatatt
    53041 atactgaaaa atataaagac ccaacagttc ctataaatat agaaaataca agtacacaaa
    53101 atttagatgt aacaaaatta aaagtttatg gagaagctaa ttcaacatta tatatggggc
    53161 aatatgctaa aataaaaatg gattttaata gtttcggtgt attaaagcca ggagaaaaat
    53221 gtactattta tttagatata atttcattag atccatcaag aagcatgaca ttaacatttt
    53281 cttatgtagg tgataaagaa aattataaat atgaacaagg attggcaagt agaatattat
    53341 tttataaccc agatggagaa gatttttttg aatataaata actagtgggt atgtaaatta
    53401 ataagggttt tataagttta atttttttat tttcgtttta tattaactat gtggtatgta
    53461 aatgtctcga tattgctcat atgttgagtt attatattta gtttcatatg aactatatgg
    53521 atttaaaatt aaaaatactt aaaaacattt actttaatag taggtgtttt tttgattaaa
    53581 agaatattat tataatgtaa aaagtaataa ataggtaaaa tatgtaaaaa ttatatgtta
    53641 taatattttt agcaagaaga tgtaatctac aatttataga gtggagttca tgtatcaaaa
    53701 aattatcctc ccgacgttaa gaagggaggt gaatatacat ggataatttt ttacaaggca
    53761 tactagcaag tctatctgct agcttaatag tttatgtagc tagcaaacta tttagaaagc
    53821 gtaaaaaacc actcaaagcg gcaactaaga gtggttggga atttgattta aaaatcagat
    53881 tccataaaac taagtaattt ctaaattatg aactccactc tacagcaaaa tagattgtag
    53941 ctcttcttgc ttttattata tcacaaattg gtacagatat tcaaaaataa tatttttatg
    54001 atataataaa gtcatagaaa ttttgcagtg ttcgattttt tgaagaaatt aaggcttaac
    54061 agttgagata taaggcattg aggacttgtg ataagtgtta tcaattgcac tactcatggt
    54121 tcactgcaaa tttgagagat ttgtatatgt gtaggcattg gaaatattca atttattttg
    54181 gggttttata ttaactatat ggaatgtaaa tttgcctaaa agatttttag ctatcttata
    54241 taatgtagtt ttatattaac tatatggaat gtaaatcatg accatactgt attgtcattt
    54301 ttgattatga atggttttat attaactata tggaatgtaa atttttcttt ttcttaattt
    54361 tttattatgt agctttaagg ttttatatta actatatgga cttaaaatta aaaatactga
    54421 aaaacactta cttttgtggt aggtgctttt ttatattcaa acctgtgtac ttaattgaaa
    54481 tatttgttaa aatatgtaag aattatatga tataataatt gtagcaagga aaataaagtt
    54541 gaaaagctga gggtgatttt ctcatataaa cgccaaattc caatatagga aggaggtgaa
    54601 tttatatgtt aattaacttt ttattgagta tagtagctgg aattgtgtca gcttacattt
    54661 acgagaaaat aaaaaatcac ccaaacgcca atgattacta ctttaatagg tttttttata
    54721 tttgcagttc aaagaggaat attttaatta attaagaggt taagatatgg ataatttaat
    54781 aagttttata ccagagcaac tacttttatt agtagttgct cttaatgtat taggatttgg
    54841 atttaagaaa tacaaacagt tagataataa atatatctca gttatactgc tagcacttgg
    54901 tatagtattt tcaatatgga tgctagggtt taatccaagt tcaattttgc aaggaatttt
    54961 atgttgggga gttgcaatag gagcaaatca agtttacaaa cagttgaagg atggtgaaaa
    55021 gcaatgaaaa tagcaatagt accaggacac actttaagtg gaaaaggaac aggagcaact
    55081 ggctatatag atgaaggaaa agaaaacaga attttaactg atttaattgt taaatggttg
    55141 aaacaaggtg gagctactgt atatactgga aaggtagata aatctaataa ctatttagca
    55201 gagcaatgtc aaatagccaa taagcaaaat gtagacttag cagtacaaat ccatttcaac
    55261 gcaaataaaa caactctaaa tcctatggga acagagacaa tatgcaaaac taacaatggt
    55321 aaggtatatg ctgaaagagt caacaaaaaa ctatcaacaa tatttaaaaa tagaggtgca
    55381 aaatcggatg taagaggtct ttactggctt agtcatacaa aagctcctgc aatattaata
    55441 gaagtgtgtt tcgtagatag taaagcagat acagactatt atatcagaca taaagacata
    55501 gttgctaagt taatagcgga aggtatttta aataaaaaaa tagataatga tggagttaaa
    55561 ctgatgtaca aacatgcaat cgtttatgat ggagaagttg acaaaatccc tgcaactgta
    55621 gttggttggg gttataatga tggaaaaata ttaatatgtg atataaaaga ttatatacca
    55681 ggtcagacag aaaatttata tgttgttggt ggtggagcat gtgagaagat aggttccatg
    55741 gctaaagaaa attataccct gataaaaggt aatggtagat ttgatacact ttacaaagca
    55801 ttagatttta ttgatagata gattggaagg tagcaactag agttagttgt taccttcttt
    55861 ttggtggttt ttagaatttt ttagtttttc tggtgttttt ggttgataca catgccacag
    55921 agaagttgtg tttgcagata aaaatgctac aaacatagct aatacaccac acgctttcat
    55981 aaaattatat agaatctttt tcatgttagt atctccttaa attatttttt atttataatg
    56041 atttgaataa ttagtaataa atttatccaa agtaaagcta aaaaagtgta tacaacataa
    56101 ttgtaaaaag ataagtattt gcatcctaaa agaataatca agaataaagt tatagatata
    56161 gttcttgaca gtaacttatt ttttttatat tttgtttcat tcagtggatt gttagtgtga
    56221 catactggag caaaaataaa aatgattaga aagttaaaac tagacaatat cactaaaaat
    56281 aacggaatag gattagaatt atttaaaatc gttcccaaaa ccatagagat aaaaaaaata
    56341 ctattgaaaa ttagtataca gtttttattg ctatttgcat gatatccacc tgaaaattgt
    56401 cttattatac aaaaagatat taagaatact atagaataaa tcaatctacc taatattaaa
    56461 cctatcatta atgaagatag agtatttatt atggaagata gtaaaatttc taatccatat
    56521 gaacagattt ctaaatcatc tttttctatt acttgattta aaacaaacaa aaaagctatt
    56581 ttatcagcac aagattttat catttagttt tctccttaaa taattttatt aataaattat
    56641 ggatattaac tattctcata tgcaatattt gaatcttctt gtgattttct aattggtatc
    56701 atcatattta aacaaaataa attttcagag aaatttatag aaattgcacc atcatatttt
    56761 tctaaagaat tttttatact ttttattcct aatccatgaa actctttatc ttcttttgta
    56821 gttaaaaaag cattattttt taataaaata tcatttgatt ttgtattagt aatttttatt
    56881 acaaagaatt tatttaatac cattccctgc aatttgattt ttttatcatc atctttgatt
    56941 ttttcacaag cttcgattgc attatctaat gcatttgcaa atatagaaca tgtatcttct
    57001 tccttaataa aattacaatc actaaagttt atattaattg taaaatcaat tcctttttct
    57061 atacaaagtt cttttttttc taataatata atgtttaaag taatatttcc tgtatcaaat
    57121 acagcactat attcatcaag c
//
LOCUS       DAEPXF010000005        55811 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain HB27
            SAMN13376389-rid12479423.denovo.005, whole genome shotgun sequence.
ACCESSION   DAEPXF010000005 DAEPXF010000000
VERSION     DAEPXF010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13376389
            Sequence Read Archive: SRR10518432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 55811)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 55811)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001069624.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001069624.1
            Genome Coverage       :: 315x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/15/2021 01:54:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,165
            CDSs (total)                      :: 4,076
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 89
            rRNAs                             :: 10, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 10 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 19 of 69
            Pseudo Genes (incomplete)         :: 44 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 10 of 69
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..55811
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13376389-rid12479423.denovo.005"
                     /strain="HB27"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="China: Hebei"
                     /collection_date="2017"
     gene            complement(176..3511)
                     /locus_tag="KRL67_000288"
     CDS             complement(176..3511)
                     /locus_tag="KRL67_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HBH1521195.1"
                     /translation="MGNFDFLQKKKNFNSFNTACLEAEKSILVSPSTCATITRRALEL
                     AVKWLYANDSDLVLPYQDNLSTLIHNNSFIELIDYDMLPLLKYIVKLGNLSVHTSANI
                     ERGEAILSLNNLHQFVSWIDYCYSDEYTAEDFNEDLLLHGEEKRTRPDELKNLYERLS
                     SKDKKLEEVIKENEELRKSLTEKRKVNTVNYDFKIDEVSEFETRKRYINIELKLAGWE
                     FGKDIGEEIEVQGMPNESGVGYVDYVLYGENGKPLAVVEAKRTSKDPKIGQQQAKLYA
                     DCLEKRYSQRPVIFLTNGFDMYIWDDYSDRKVCGFYKKSELQLMIDRRKSKKSLTSVT
                     INDEISNRYYQKEAIRAVCEALENKQRKTLLVCATGTGKTRIAISIVDVLSRHNWIKN
                     ILFLADRKALVKQAKKSFTKLLPNLALCNLLDSKDSPEDARMIFSTYPTMMNAIDDTK
                     SKDGKRLFTPGHFDLIIIDESHRSIYKKYKSIFDYFDSYLMGLTATPKDEIDKNTYSV
                     FDMENGVPTYAYEYNKAVEDGYLVDYTSIEFKTKIMEDGIKYDELSDEEKEEYENTFN
                     DDESIGDEIGNNAVNEWLFNSDTIDLVLNKLMTEGLKIEGEEKIGKTIIFAKNTKHAR
                     AIVERFNKLYPKYGGNFVKAVDYSINYVDSIIDDFSDKNKLPQIAVSVDMLDTGIDIP
                     EILNLVFFKKVRSKTKFWQMIGRGTRLCPDLLGVDMDKERFLIFDFCNNFEFFKFNPK
                     GFEGNKAETLTEKLFNIKVSMVKELQDIKYIEGEYAELRKELLEELITSVKALNEDSY
                     IVRMNLSYVHKYKNENVWSNIGAVAQNEIREYISPLITSYSDDELAKRFDVVMYNIQL
                     AYLQNNNASKGIRHVMATAEKLSKLGTIPQIQQQKYTIEKAMTEDFWEDSDIFEVEEV
                     RISLRELIKYLEKSSQKIYYTSFEDMIVAEDRNDSVYNANNLKNYKKKVEYYLNSHKD
                     ELAIFKLRNNKKITKQDVETLEEILLKQLGNCDDYKKEFGNTPVSQLVRKLVGLDREA
                     ANEAFSEFLNNKSFNTKQIHFVKLIVDYVVKNGFIEDNKVLMEDPFRTVGSIIDLFEN
                     HIEERNKLIKTINKIKENASEIG"
     gene            complement(3561..3989)
                     /locus_tag="KRL67_000289"
     CDS             complement(3561..3989)
                     /locus_tag="KRL67_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH1521196.1"
                     /translation="MVIRNKLSWNSIEEVNRDFGSCLYDLQNFKILYDKEEMPELWAR
                     YIKEGFKSYMVSFLELTKAMLYYKSIDLNIKSKNFYDHLLACEYHNLLPKNSSIVIET
                     LRKLRNDDSHGYDIPQFEDMYELFTENEEVFVSIRNSCKK"
     gene            complement(3979..4359)
                     /locus_tag="KRL67_000290"
     CDS             complement(3979..4359)
                     /locus_tag="KRL67_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HBH1521197.1"
                     /translation="MRNISNEIKNSIINVFPKENTLAIIIFGSYGTDKHTSESDIDIA
                     WIPIKKVPITELATKTEALRNILDIDVDLKIVTDNYTIALKKAILEGEVIYESKDFKD
                     YINKFYFENSDVIDILDWRDMYGN"
     gene            complement(4392..4700)
                     /locus_tag="KRL67_000291"
     CDS             complement(4392..4700)
                     /locus_tag="KRL67_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH1521198.1"
                     /translation="MNQIELDLYHDMVNIYKEADLQCNYKPTRFLQLINNKGALLAAK
                     ELINKEGATEGFTRLWECKRLDLSLEVLVLKEKYKELFTDEEREICINRLKDYGYKFN
                     "
     gene            complement(4733..5905)
                     /locus_tag="KRL67_000292"
     CDS             complement(4733..5905)
                     /locus_tag="KRL67_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HBH1521199.1"
                     /translation="MEYRLNEVFEFIRNGASIKQFDTKFGYPITRIETIANSYIDTNK
                     MGYADIKELGKLEDYLLKNGDILMSHINSEKHLGKSAIYEDINKKIIHGMNLLCLRPD
                     KEVCYPKYLNYFFNSNEFRRQLPRIIKKSVNQASFSVNDLKNLKVDLPEMEAQKDISE
                     VLDKAQELIDKRKEQIEVLDELVKSRFIEMFGNFKEQKFVLLKDCTNFIDYRGKTPIL
                     SENGIRIINAKSVGNGVFKYINEYISENTYNSWMKRGFPVAGDVLFVTEGHTFGNVCR
                     IPKDLKKFAMGQRIITIQGDKKILNNAFLAQYMQTKAFRVEIDKYKTGSSAQGIRSKE
                     LQKILIPVPSIELQNKFEEFVNQVDKLKVGIKTSLKELEDNFNSLMQKAFKGELFN"
     gene            complement(5909..7372)
                     /locus_tag="KRL67_000293"
     CDS             complement(5909..7372)
                     /locus_tag="KRL67_000293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent DNA methyltransferase"
                     /protein_id="HBH1521200.1"
                     /translation="MITGELRSKVDKIWETFWTGGLTNPLEVIEQFTYLLFIKGLDEV
                     ETKNEAEATLLGVSFEKIFDDEHQHLRWSKFSNEGNSEKMYEIVQNEVFPFIKKLHGN
                     KESAYAKYMGDAIFKIPTPLMLSKIVDGISNLELSKDRDTKGDLYEYLLSKVATAGTN
                     GQFRTPRHIIDMIVRLIKPTPEDIIVDPAAGSAGFLVSSQQYLRDNHSSLFLVQGLKE
                     HFNNGMFYGFDMDRTMLRIGAMNMMLHGVDNPNIEYKDSLSEVNTDKDKFTLVLANPP
                     FKGSLDYEAVSADLLKVTKTKKTELLFLALFLRVLKIGGRCACIVPDGVLFGSTGGHK
                     SIRKEIVDNHKLEAIISMPSGVFKPYAGVSTAIMIFTKTGTGGTDKVWFYDMKSDGYS
                     LDDKRNEIKDSDIEDIIERFDNLDGEVDRKRTEQSFFVDVEEIRENGYDLSINKYKEV
                     VYEEVVYDAPSVIIGRVRELEKEIVDGLEELERMLEV"
     gene            complement(7778..8650)
                     /gene="gatY"
                     /locus_tag="KRL67_000294"
     CDS             complement(7778..8650)
                     /gene="gatY"
                     /locus_tag="KRL67_000294"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatY"
                     /protein_id="HBH1521201.1"
                     /translation="MALVTTKQILLDAQEGHYAVGAFNVENMEMVMAVIEAAEELKSP
                     VILQTTPSTIKYAGLDYYLANVKVASEKASVPVAMHLDHGSSFGLAMQALRVGYTSIM
                     IDGSHESYEDNISISKSVVDACMPSDIPVEAELGKVGGKEDDLDGGDGGAYTDPLEAK
                     EFVERTGVSSLAVAIGTAHGLYKGEPKLDLDRLSEIREVVSVPLVLHGGSGIPDEVIK
                     ESIKRGICKVNYATELRIAYSNGVKDVLNSDPEVIDPKKYGKKGLESVKDFVKSRMEV
                     CGCVGKAIEIVKCY"
     gene            complement(8670..10586)
                     /locus_tag="KRL67_000295"
     CDS             complement(8670..10586)
                     /locus_tag="KRL67_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIABC"
                     /protein_id="HBH1521202.1"
                     /translation="MGKKMILGVTGCPTGIAHTFMAEEALKNSARELGCDIKVETNGA
                     IGVENKLTAKDIEMADAIIVACDKNVDMDRFNGKPVIEVPVKEGIHKANELIQRCIDG
                     KVAIRKGNLSSSKIAEEGNLSFGQKLYKDLMNGVSHMLPLVVAGGVLTAISFLWGIYS
                     FDPTSEQYNQIAATLKSVGGYSMNLMVPVLAAFIAQSVSGRAGMLAGLVGGMISFDTG
                     SGFLGGIISGFLAGYLVKLFVHLLRKLPRQLEGLKSIFIIPIVSVGLVGVAMLLLGGP
                     CSALNNAMMNFLSGLQNSSPIILGIVIGCMSAFDMGGPVNKAAYVTGTMLLGQGNYLF
                     MAGVSAACITPPLVIAIASTLFKNRFTEEDRAAGLINYILGSTHITEGAIPFAAKNPL
                     KVLPVLMIASSISSILTYIMKIEVPAPHGGFLVLGLVNKPLLWVGCIFVGSLVGAVLY
                     MMVTPKVVNEKNTIENTNATKDEHKNKSAESENVFNNISLYSEETVILDVKGKNKTDV
                     IDEMIGILDKSGVLLDKKKFKEEILKREEISSTGFGMGIAIPHAKTDAVKVPRVAVGV
                     SKEGFDFESEDGSLAHIIFMIAATDNGDNLHLKTLSQLSSKLMNEEFLNELLNSKTSR
                     EIVSKLNNEEIKSV"
     gene            complement(10589..11509)
                     /locus_tag="KRL67_000296"
     CDS             complement(10589..11509)
                     /locus_tag="KRL67_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase family hexose kinase"
                     /protein_id="HBH1521203.1"
                     /translation="MIYTLTTNPAIDMNISTNGIKRKLVNRTSNAVYSPNGKGLNVTF
                     VLGHYGIESKILGFFGGFSGKYIVEESEKRGFDVLPTWVEDTTRINIFLNDGSDEFKF
                     VNSGSYVNEKQKLDMLEKIQSLEDISYLSISGSLPPGIDDNYYEDIFKICKNKNIKTI
                     LDISSPKLKELLQYNPYLIKPNDEEIKDIFGIIVRDEEDIKDVLKLLHLKGAQNILLT
                     LGEKGSYFYNGKSIYYASAQPVTVISSACAGDSALAAFLSIWLENSEHIEEALKRSAA
                     TGASVAESNGIGNLENVEDYIKNIRVRKVV"
     gene            complement(11611..12363)
                     /locus_tag="KRL67_000297"
     CDS             complement(11611..12363)
                     /locus_tag="KRL67_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HBH1521204.1"
                     /translation="MIIELNKSNSENLTKTELEIIKFINENESILPELSIVEIAHETY
                     SSPATVSRAIRKCGLNGFNELRYRLTVKDENDDIQNMGDIINKSFIEAQRVIEQISLT
                     TLINIIKTINKSSKIYILARGLTEYVAKEFSLKLQLLDFNVVFINDPNIMKIKSKSIK
                     KDELIIIFSLNGNTIELVESAQNANLVGAKVITCCCSESAKLIKYSDLYIVGYKHEHI
                     SISEYEVSSRLPLYIISRIIIDYMVRYNNSEN"
     gene            complement(12755..14200)
                     /locus_tag="KRL67_000298"
     CDS             complement(12755..14200)
                     /locus_tag="KRL67_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HBH1521205.1"
                     /translation="MIKLGKFPKGFLWDGAVAANQLEGAWNEGGKGWCLADIHMYDKD
                     RDISKPYESDMTIERIKFAMKDNNGYYPKRSGIDFYHTYKEDLKLLKELGLKCFRFSI
                     NWARIFPNGDDLEPNEEGLKFYDNLINEVIDNGMEPIVTLLHYETPLNLVLKYKGWSN
                     RKVVDFFVRYAELILNRYKDKVKYWIVINQINLIHYESFNSVAFCVDQVDNVEEARYQ
                     AIHHQFIATALTKKLGKEINSDMMIGMMMADCTSYPEDCDPENVVFALKRNRLQYFFT
                     DVSFRGEYPVYIKRFFKENNINIVMEEGDDKILKENTMDFLALSYYYSTTVSASKNTM
                     NPTDNIKNPYLKANPWGWAIDPKGLYNTLSQYYDRYQKPIIIAENGFGMHDKFEDGKV
                     HDSYRVDYLEKHIEQIGEAIADGTEILAYCMWSPIDIVSCSSQEMEKRYGFIYVDIDN
                     NGNGSKERCKKDSFYWYQNVIRTNGEELREK"
     gene            complement(14222..14575)
                     /locus_tag="KRL67_000299"
     CDS             complement(14222..14575)
                     /locus_tag="KRL67_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HBH1521206.1"
                     /translation="MTIEEHIIDEELVEVAMQIILRAGEARTEIKHALNDLEKFDYRN
                     ADLKLAKAKEFMTEAHRAQTKIIQGEAGGEKRAHSLLFAHAQDTLMTIFSELNITMSL
                     VGIVRSIEYKVNNSK"
     gene            complement(14604..15851)
                     /locus_tag="KRL67_000300"
     CDS             complement(14604..15851)
                     /locus_tag="KRL67_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBH1521207.1"
                     /translation="MGRVFMNKFIDFLSEKFTPVVNNMTKNIWVQSVQSTIMKVLPMV
                     FVGSLVTIVSVLKNYISFLPDLSPINQYTFGLLGLFIAFLLPMEIMRNKKFESMSVVA
                     GLAGAGLMLMMIRPEITNEGAIFNFNRFGGEGMLVSLVAGLFSGLIMSLFGSFSFFGE
                     DSALPDFVSKWFDNMLPITVIMFVGWGLIYGLNFDMFEIIVRVFKPVSDIAQTLPGFV
                     LLNFLVVFIYSLGISGWVLSPITYSIQLGAIAANAAAVASGGVATNIYVYEVIQTGWI
                     TLGGLGATLPLVIMMCLSRVGRIKAIGRACIVPSIMNINEPVIFGAPVAWNPFLMLPM
                     WIGTIVISITTYLSMSMGFVTIPSKVFNLWYVPFGISTFMVNQDVRGLIVLAINIVLL
                     FMIWYPFFKAYEKEELKLESQEI"
     gene            complement(15859..16176)
                     /locus_tag="KRL67_000301"
     CDS             complement(15859..16176)
                     /locus_tag="KRL67_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBH1521208.1"
                     /translation="MKNIRVLLACGSGASTGFMATSMRKSAKKKGIEADIKARSESEI
                     DKYLSEIDCLLLGPHLDYMKEEIQQKANEYNVPVACISKTAYGRLDGAKALEEALELI
                     SNK"
     gene            16418..18331
                     /locus_tag="KRL67_000302"
     CDS             16418..18331
                     /locus_tag="KRL67_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBH1521209.1"
                     /translation="MKTSKKELFLNFIKTQHDWIDSSTLANYLNVSTRSIRKYVNEIN
                     SNGEFILSSKKGYKVNLNNNCQTKIDSSENISPDNRLNLILKELIVNSNGINIFDLSE
                     ELFVSPATIEGDIVKANKFIGSYNLKIKQSKFLLKLVGNESAKRKLMSSIIFKETGSD
                     FLSLFDVQKIYREYNLTKLKENIIYILKKYNLFINEYAINNILLHLMITIDRIKKNNY
                     IESVEVVNYIDNNIDINIAKDISNFLESEYNITLTSAELYYLVFQLTNKTTVLNYNQM
                     DTKSLSNYIDEHFVKLTKKIIKNVYDLYLIDLSDEEFVVKFTLHVKNLISRAKNNQVL
                     RNQIPQKLKDSYPLIYDISVYICNQIQTLENVDIDEDEISYISLHVGSFFDRQKLLEY
                     KVLCALITPNYYDLQFKIVRDLEKRFNESIEIIQIFSDTHNLDFDNKVDMVITTLPIS
                     DRCPIPFVYVNLYLNRKDYDNIQSKFIQIKDRKNILTVQNHLEMYFSESLFMKNIYLD
                     SAKDYIKFMGNILCKNKYVKPNYIDDVLIREKMSSTAFNNNVAIPHSMKMDALKTGVC
                     LIVNDKPVKWGEEKVQIIAMIPINEKEKEKFNYIFESFIEILSEWNNIKELTKADNYS
                     SFMNRIAYLIQNI"
     gene            complement(18454..19608)
                     /locus_tag="KRL67_000303"
     CDS             complement(18454..19608)
                     /locus_tag="KRL67_000303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HBH1521210.1"
                     /translation="MKILISIDSLKGSLSSIEAGNAIKKGILKVKEDVQVKILPLADG
                     GEGTVDALVQGMNGKKETIEVTGPIAKKVDATYGLLKDTSTAIIEIAQASGLTLVPTE
                     LRNPLYTTTYGVGEIIKEAINKGYRNFIVGIGGSATNDAGVGMLQALGFEFYDENNKL
                     VGLGGKVLNEIRHIKIENRLKELDECKFKIACDVNNPLFGKNGAAYIYGSQKGATSEI
                     IEELDNGLRNFSKVVKNYLSKDVANVEGTGAAGGLGFAFLAFLNSKLESGIKIILEEI
                     KLEEELKDADFVITGEGRLDNQTAMGKAPIGVAKLAKKYGVKVIGLAGATTEDAVKCN
                     EEGIDAYFSIVNRAMTIEEAMDKATASENMTATTTQIFNLITSIQQSKNM"
     gene            complement(19890..22505)
                     /gene="mngB"
                     /locus_tag="KRL67_000304"
     CDS             complement(19890..22505)
                     /gene="mngB"
                     /locus_tag="KRL67_000304"
                     /EC_number="3.2.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosylglycerate hydrolase"
                     /protein_id="HBH1521211.1"
                     /translation="MKKNVHIVPHMHWDREWYFSTEESRILLVNNMEEIMDMLENNHN
                     YPYYVMDGQTAILEDYLAVKPECKERVKKLVQEGRLIIGPWYTQTDEMVVGGESILRN
                     LLYGIKDCDEFGEYMKIGYLPDSFGQSAQMPQILNGFDIKYSMFWRGCSERKGTNKTE
                     FNWKSDDGSSVLVQILPLGYAIGKYLPMNEDELRTRMDKYLPVLDKGATTDHIILPNG
                     HDQMPIQKNIFEVIYKLKECYPERKFFLSRYENIYKELEKNEDIDTIKGEFLDGKYMR
                     VHRSIFSTRMDIKSANARIESKITNILEPLASIAYSLGFEYHHGLIELIWKEIMKNHA
                     HDSIGCCCSDKVHQEIMNRFFLAEEKVDQLIIFYKRRIVDAMSCEMALDKLVAFNLMP
                     YDRNEIVSAQVITKMKAFEIFDRDNNKLDFEVVHKEEIDAGLIDRQIVHYGNYDSFMV
                     YTINFKDKIPAMGYKAYMIKEVEYMLEKAYEVCDMVDNDFYTIQVNENGTLKIFDKKL
                     NKTFENVLLMENGGDEGDEYDFSPLPNEKLIFNDNIVANHTIKKNRFNNEININYRLK
                     VPKDIQARKNNREDSFIDFDIVINVPNDKAIIDVVFNINNQARDCRVRTYIPTNIASK
                     FSVSDNQFGHIKRSVYDDAMEVWQQEEWSERPDSIYPMLTFVGLSDKEHGVAVLTNST
                     REFEIVGEKFDTIAITLFRSVGFLGKEEMVRRPGRPSGIKLPTPDSQMIGDIKIDLAI
                     VTHEKSTLEANVANMAKEYLTKIQTYNKMPYNAMKLNQSEIEVDYSYSLLKETNKDLV
                     LSVIKKAEKEEALVVRFYNSKEETKSANIVFNKNIKIAKWINLNEKELEFISIDNNSV
                     NVECLMNQVKTILVK"
     gene            complement(22648..24573)
                     /gene="mngA"
                     /locus_tag="KRL67_000305"
     CDS             complement(22648..24573)
                     /gene="mngA"
                     /locus_tag="KRL67_000305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS 2-O-a-mannosyl-D-glycerate transporter
                     subunit IIABC"
                     /protein_id="HBH1521212.1"
                     /translation="MEFKKITNENLVFLKQKIKTKEDAIKFLARKLYDEGKLTSYEEY
                     LEAVMYRETLSPTGFEKGLAIPHGKSKSVKEAAFAAMTLEIPIEDWESVDPNNKVELI
                     FLIAVPESEGGNTHIQILSELVTRLSNEDYMNMLLNSKTKKELFNNLDIKEKKEQIKE
                     EIRIQNESKKIVLAITACPAGIAHTYMSAEALIKAGKEIGVDVYVEKQGANGMVDPHT
                     PDIIKRADAIIYATDVAPKNTERFEHLPNVKTSVAAPLKRAKEIIYEALEVAQKQGKG
                     DYIEKSSDISYCEKSSWKKDVKSAILTGVSHIIPLIVAGGMILAFAVLASQAFGLQDL
                     YNQEGSWLWQLRKLSSGMLGTLMVPVLSAYMSYSLAEKPGLGPGFAAGLAADLIGSGF
                     LGGMLGGLLAGYIVKFLKKNIPAKGTLAGFVSFWVYPVVGSLVVGLLMLFVVGKPVAA
                     LNNGLINWLNGMTGVNAILLGAIIGAMVSFDLGGPINKAAYTFAIGAIASGNFIPYAI
                     FASVKMVSAFGVTIATILGRDLFSESEKEIGKQTWILGLAGITEGAIPFMIEDPIRVI
                     PSLVAGSAITGSIVAFFNIGLQVPGAGIFSLALLEGSMSGFMAASIWFFAAVLGAIIS
                     AMLLIITRKQKNQRKIY"
     gene            complement(24703..25428)
                     /locus_tag="KRL67_000306"
     CDS             complement(24703..25428)
                     /locus_tag="KRL67_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HBH1521213.1"
                     /translation="MEFYERAKKYEYKFTDTEDMIIEYMRKNKEEIVKLSIQSLADKF
                     FTVPNTIVRLCKKMEYDGFSQLKNNLKEELKNKNKESKYGFESDIKKTLELIDYEKIN
                     KVADIIQKSRNIIVYGIGQNIPLCEILVKEIRGSHNNITYYSQRHEVLHDIGKLGEQD
                     MFFALSLSGETKEVIEAAKIAVDKGCKVVALTHICENQLQQIADISLYCYSTIKQVNG
                     YNISDKVPIMMIMRAIIEQYWLR"
     gene            complement(25691..26470)
                     /locus_tag="KRL67_000307"
     CDS             complement(25691..26470)
                     /locus_tag="KRL67_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ChbG/HpnK family deacetylase"
                     /protein_id="HBH1521214.1"
                     /translation="MHKMKKLVVRADDIGYSEGVTLGIISSYKNGIVTSAGIMVNMPF
                     SRQAIEMVKNDDACFGLHVNLVIGEPCCKEHIKIKNLLDEGGKFVSSKIRRTQLLTGK
                     EVFNYEEVYEEVCAQIETYKLYMNKLPEYIDIHGIEEDVSISAMCDAAKEYGIPSCPY
                     YLDSCISMPSKYPQNDFYKLNKSFVKLFEEKYINLDKEITLMVTHPGFIDYSLIKSSS
                     LVQERVYEHAMLCDKEVKMWLKRNNIELVDFKDIFKANNYI"
     gene            complement(26463..27890)
                     /locus_tag="KRL67_000308"
     CDS             complement(26463..27890)
                     /locus_tag="KRL67_000308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HBH1521215.1"
                     /translation="MMQQLSLLGKTMLVPIAVMPAAGILGLLFGPNMLNVPAISNISN
                     IVFSNGDYLFLLGAASAYSKSKDKTSVMVAAVVAYMIFKSNLELLNDTLNAGVFGGII
                     VGACIAWTYNRTYKVKVPAFLSFFAGEKCVITLSPVVALILSYVFSLLWIYPQAAMNQ
                     FGFWLGGAGAIGIFLFLFCNRALIPTGLHQVLNAYILFEMGEYTTSTGEVIRGEIPRF
                     MAGDPNAGFFWTGFYIIMMFSIPAISYAIYKTAQDKKKQEVKGAMTAGALTSFMATVT
                     EPIEFSFLFASPKLYIIHSFYSGLGGVVLYLLGARLGSFNGASIIDYVLSFSYGDKAW
                     LVIPIGIVFFVLYYVTFKYIIIKDNVQTPGREVEIEIGDKVSEKEKNLKLSHGNFSYM
                     AKQIIKNCGGYENIVTLNNCMTRLRLEVKDATILNDDNIKKTGAKGVIKLSNTSVQII
                     IGTDVVKVKDEMEMQLDELRKQADA"
     gene            28281..29003
                     /locus_tag="KRL67_000309"
     CDS             28281..29003
                     /locus_tag="KRL67_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBH1521216.1"
                     /translation="MAKNKFSEIYEVLKEEILDGKYTSNMMLPTELQLIERFSCSRNT
                     VRRAISQLNAEGYVQSIKGKGVVVLENSCSNDFFLNMHNFKGVESIVEDKKVNTATSV
                     LHFSKILIDNKLSKKTGFKVGSEVYYLHRLRYIDNIPKILDINYFLCSIVKNLDVSIA
                     QGSIYKYIEESLGTKIVSSRKIFKIEKATELELKTLPLNDYNCVGVIKNSVYTDDGKL
                     FEYTESKHTPETFVFMDVTQRY"
     gene            29199..30866
                     /locus_tag="KRL67_000310"
     CDS             29199..30866
                     /locus_tag="KRL67_000310"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HBH1521217.1"
                     /translation="MKNWWKKATVYQIYPKSFKDSNNDGIGDINGIIEKLDYLSSLGV
                     DLLWLTPMYVSPQRDNGYDIEDYYNIDPKYGTMSDFEKLLKEAHKRDIKIMMDMVLNH
                     TSTEHKWFKESKKSKDNPYRDYYFWKDAKPDGSVPNNWISRFSGTAWKYDETTNQYYL
                     HLFEETQADLNWENEKVREECYKILEFWADKGIDGFRLDVVNLLSKTPGLPDDPITGP
                     KGDGRTHYADGPRIHEYLHNMNQKVFKPKNIVTVGEMSSTTPEECINYTRENREELSM
                     VFSFHHMKTDYKGGAKWTNEMFSLDKLKKAQSDFQYKMYEGKGWNALFYSNHDQPRAL
                     SRFGDDRFFRQESAKMLAITLFGLQGTTYIYQGEEIGMTNAYFTKIDEYDDAESKNAY
                     YHMIKSGIDEKEALLILQQKSRDNARTPMQWDNTIYKGFSNHKPWLKVNNDNISVENE
                     LNDSRSVFYTYQRLIKYRKQYDIFTEGTYRLLDENHKNLFVYEREYKKQNLLVISNFT
                     HDNVVYKLPETYLNKLNYTILETNYTRDIIENEMEIKPYESIMIYYK"
     gene            complement(30962..32281)
                     /locus_tag="KRL67_000311"
     CDS             complement(30962..32281)
                     /locus_tag="KRL67_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBH1521218.1"
                     /translation="MEKGIKLGFWSIVLLGINAIIGSGIFGLPGDAYTDIGPASILVL
                     VFCMLLAVSIALCFAEAGSWFDTDGGPYLYAKEAFGDFIGFEVGFMKWIVSMIAWATM
                     ANFFAVTLSSVWPQAAEPLTKNIIIGILVVGLGIINFMGMKQSKHLNNIMTIGKLLPI
                     VLFIAVGLFFIKGSNFTPFVIIQKGQSASSAFVAVSITLFYAFTGFESLAVAAKDMEN
                     PKKNVPKALVMVMFVVSVIYMLILGISIGVLGNGLAGSATPVADAAIKMLGPIGGYII
                     TIGTIVSVGGINIASSIFTPRSAAALVEQGLMPRSIRKTNKNGAPYIAIIVSVIGTLL
                     IAWSGSFTTLSQISVVSRFIQYIPTCLAVLILRKKYAGKDVNFRIPGGAIIPIFAVII
                     SILLLIKAGIDEPMKIVWGLGGMIIVVPIYFYMTKVYSKKYNDVEVK"
     gene            complement(32322..33068)
                     /locus_tag="KRL67_000312"
     CDS             complement(32322..33068)
                     /locus_tag="KRL67_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HBH1521219.1"
                     /translation="MKKVVKKKYPVIGISGNLLIDEGGMFPGYERAYVNNDYIQSVVM
                     CEAIPYIVPIVYDDEIIKEQVSNIDALILSGGQDVNPLIWKEEPHNKLGAISPKRDSF
                     DMKLLKHALDMKKPVLGICRGEQIINVTEGGSLYQDLSLIEGAYIKHNQQHLSNVPTH
                     TVQIKEGTKLYEILGEKEVLVNSFHHLVVNKVAPGYIVSATSKDGLIEAIEKEGSEFV
                     IGIQWHPEMMTRDYDNMKKIFMAIVKEASK"
     gene            complement(33312..35321)
                     /locus_tag="KRL67_000313"
     CDS             complement(33312..35321)
                     /locus_tag="KRL67_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBH1521220.1"
                     /translation="MKTIAVVTDGLSKLECFLNKNLEMIFKDKVIIKNYYLNKITEKE
                     LIKDDIILVMIDDRVLKIKKYVEDVSKIITINRSIKQKDIYKLFSLPEGIDVLVVNDN
                     KHTVLETISNLYNIGINHLNFIPYNPNEEYRNIKVAITPGEASLVPKYIKEVIDLGHR
                     YIDSSTFIQIIAKLKLDDKEITERLIKYTDEIISLYSGINTKYKELTIKADELNSIIN
                     LSNLGMAMVSDKGNIIICNNSLSDILDIQSNIIGKNIDELENENIKKIFSLSKAHDEV
                     IKFNNKYLNVNKYNIESFGRVTGYYFCIQEITYIRKLEQNLSKKLREKGQVARYTFDD
                     IKTVSTKMERTKKLGMKIAESDYTILITGESGTGKELMAQSIHNASPRHSQPFIAINC
                     AAMPENLLESELFGYEEGAFTGALKGGKKGLFEQANNGTIFLDEIGDMPIYLQSKLLR
                     VLQENQVMRVGGENVIDIDVRVIAATNKNLLEMIKGDRFRADLYYRINVLPINIPPLR
                     ERKEDIEVMLKHFMKRKRAISQDVQDIINTYEWPGNIRELKNTAMYINIMSSEDVILI
                     NDLPHNLLNITEDFSKEIRILNDRTSIEKVKLVMECLKNANNLNKNIGRNGIVNLINE
                     LGYSITEGEVRGILNILKELDLIICESGRKGSELNRRGKNILNSI"
     gene            complement(35759..37192)
                     /locus_tag="KRL67_000314"
     CDS             complement(35759..37192)
                     /locus_tag="KRL67_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HBH1521221.1"
                     /translation="MALRKDFLWGGATAANQCEGAWNLDGKGPSCSDMCTGGSQKVSK
                     RITRVIEKGTFYPSHEAIDFYHRYKEDIALFAEMGFKVFRFSIAWTRIFPTGMEKEPN
                     EAGLKFYENVIDECLKYNIEPLITISHYEIPFAITEKYNGWVSRDVIDLYMNYCETIF
                     KRYKGKVKYWLTFNEINSATMPMGGYLSQGILNEGTTDFINQVDRPQLRFQGLHHQFV
                     ASAKAVKLAHETDSNYQVGCMQIFATMYPYTCNPDDAVKTQRDSRVMNYFCGDVQVRG
                     EYPTYMNRYFGENNIEIKMEKGDLEILKEGCVDFYTFSYYMSTCVSSDSKEDNTSGNI
                     LGGVKNPYLKSSEWGWQIDPEGLRYALNEIYDRYRIPMMVVENGLGAYDKKESNGVIN
                     DDYRIEYLKAHIEQMKEAVEDGVDLFGYTPWGCIDLVSASTGEMAKRYGFIYVDKYDD
                     GSGDLSRIKKKSFDWYKQVIESNGERL"
     gene            complement(37279..39195)
                     /locus_tag="KRL67_000315"
     CDS             complement(37279..39195)
                     /locus_tag="KRL67_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HBH1521222.1"
                     /translation="MRKYEQLSKEIIANVGGKDNISSLSHCITRLRFKLKDESIANDD
                     VLKKMDGIVTVMKSAGQYQVVIGNHVPDVYAVVCDLAGLGGDTVDNSDSDAPKGLLNK
                     FIDIISGVFQPILGVLCAAGMIKGFNAVFSALGLYGETDGAYIMLNAIGDAMFQYMPI
                     ILGYTAAKKFKLKPFIGMLIGAVLCYSGIQLDTLASTGQPLYNLFTGTIFESPVYITF
                     LGLPVIAMNYTSTVVPVILIVLLAAKLEKVFAKIIPDVVKNFFVPMAVLLVSLPLGFM
                     VIGPIATYASNIVGNGFLALFNFSPILCGALTGLLWQVLVIFGLHWGLVPIAMSNLMT
                     MGFDTILVGAFVPSFAQTAVVAAMYFKLKDKKIKELCIPAIISGICGVTEPAIYGITL
                     PRKTPFIYSCVGGAVAGAVMGIMNVKSYVIGGLGIFGIPNYINPTTGDMTGVYATIIA
                     IIVAIVVGFVLTLFLWKDDAIVEISKDDNGQGVVEKNKREVIYSPIRGYVKVLEQMQD
                     AAFSTGVLGKGVAITPKEGKVVAPFDGTVMTLFPTKHAIGIISDNGCELLIHVGLNTV
                     QLEGKYFKSFVNQGDKIKKGQTLITFDIESISKEGYCLETPVVVTNYSDYVDIIENTQ
                     KNVNNSQELITVLA"
     gene            complement(39269..40129)
                     /locus_tag="KRL67_000316"
     CDS             complement(39269..40129)
                     /locus_tag="KRL67_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HBH1521223.1"
                     /translation="MIIEKILNNNVIITTDENHKEIVVMGRGLAYKKRTGDHISKDKI
                     DKIFKLSDPNISDKFKELIADIPMRYMELSDEIILYAKEKLGKRLNDSIYISLTDHMY
                     TAIERAREGVNVKNVLLWDIKRFYKNEFKIGLEALDYIEKKFEIRLSEDEAGFIALHI
                     VNAHMDQSIKIIYEITQIIQEIANVVKYHYRIVFDEDSVYYHRFITHLKYFAERIVSN
                     NLHESNEDDLLNVIKVKYKNAYKCIEKLDEFIHKKYNYDLTDEEKLYLTIHVERVVSK
                     SIKSDINPNN"
     gene            complement(40480..41667)
                     /locus_tag="KRL67_000317"
     CDS             complement(40480..41667)
                     /locus_tag="KRL67_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HBH1521224.1"
                     /translation="MIESIKKRAYEIENELGKEIEEVCNFIFNNPELGEEEYISSKYL
                     VEKMKEYGFDTTYPYCNMETAFRAELGDNDGPTIAFLAEYDALPGYGENKEPAHACGH
                     NWIAASTLGACIVLSKLKENFKGKIVLIGTPAEETTGGKCDLVKAGAFDDVDVSYQMH
                     IEAFNNINCKALAIDSLEFSFEGVSAHAASHPHMGVNALDAVQLTFAGINALRQHVKS
                     DVRIHGIVSNGGEAPNIVPEKAACKFFVRAAERSYLDEVTKKVINCAKGAELMTGAKL
                     SYRYFENSFDNIINNKVLQKITKNNLIEVGITDILEGKDGPVGSTDIGNVSQVCPTMY
                     TEIALDISPMVYVHEKEFLNYANSGEAYDKLHKAVKAMVGCALEIYLEDGLLDEIKKN
                     HLN"
     gene            complement(41681..43072)
                     /locus_tag="KRL67_000318"
     CDS             complement(41681..43072)
                     /locus_tag="KRL67_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HBH1521225.1"
                     /translation="MSSLKKKKFTMPQTLTIIFLLIVLMAILTWIVPSGNFERVDIDG
                     RSVVVAGTYEKAPSNPQGITDVFTAPINGFIDAAEVVGFVLIVGGAFGIVNKTGAIEA
                     VIAHTVNKMKKFQFLIIPISMILFGLGGTTFGMSEETLPFYMIFIPLMTSMGYDSLTA
                     VATVFIGATAGFGAATTNPFSVGIAQALSQIVPGSGIEFRVVMFIIYMAISIGFVMMY
                     ANKVKKDPKKSLVHDISLNQESMVNSDSNIKEFTKREAMVIAIFTIGMAIMIYGVLRL
                     EWYITEIAMIFTAIGIISGIASGLKQDEIVNSFISGAGDLITAGLCIAFARGIVIIAE
                     NGFIIDTILNSAANLLNGLPKTIFINLTFLIEGLIAFLIPSASGLASLTIPVLAPLGD
                     LVDVSRQMIVTAYQFGIGVTNLITPTSGVLMGALAVANIPWSKWVRFVVPLMVVLTIL
                     VMVFLTIGLYLGF"
     gene            complement(43668..45368)
                     /locus_tag="KRL67_000319"
     CDS             complement(43668..45368)
                     /locus_tag="KRL67_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HBH1521226.1"
                     /translation="MHYIAWHFNKNGGNNTMKDKLQVLSGAMMIPVILLVVAGVFIGL
                     GSAFANMENVSALGLSGLIKEGSFINVFFKVINDLGFMVMRYLPIFFVTGIAFGLSKK
                     EKGWGALGGIVLFIGMHTVISTLLAANGINSDTVTAEAFMASGLGEAEAYSRSSLYGS
                     FLGIFSYDCSIFGAIIAGFVASAVHNKFVDIELPSALSFFSGPRFSMIMMFIASIVLG
                     TAMYFIWPPVGLALSKLGNWIGSSGLLGTFTFGAMDKALLPFGIHHLIAFPIEYTRVG
                     GTMEVGGAIYEGVNNIRLAQMGDPDTLSYITRNFTTGRLLIHFAILPAAALAMYKLAD
                     NANKKKAISILIPAIVTAMLVGVTEPIEYTFLFVAPLLYFVAYVPLAGLTYVFTEMFN
                     VSIMGESFRNMFPNLLQPQKVDALPLLFLIPIFFIVTYIIFTWAIKKFDIKTPGRSSE
                     EVKLYSKKEYRERQSVDAGKTAYNNAVEEEVDDKDTKLVHSIIEGLGGSNNIKNVTNC
                     ATRLRVELNSIDGFYEDGFWVNELGASGVVKKKNSVQVIFGPRVITIASKLKAVLGVD
                     "
     gene            complement(45340..46743)
                     /locus_tag="KRL67_000320"
     CDS             complement(45340..46743)
                     /locus_tag="KRL67_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HBH1521227.1"
                     /translation="MIVNKELIIDYLKSNQENIIKDIRSLVEIPSIRDESTTDINQPF
                     GIEIRNAFDKLIQIAKDKDFVVKDFDGYAIHIEYGEGEEVVGVLNHIDVVPIYNKELW
                     KSKPFKVCQKDNYLYGRGVNDNKGPLIGILYALLFLRELNEKPKRKIRLIVGGAEETT
                     WECMEHYFSVNEQPKFAFSPDGNFPIVNGEKGILYFNLRKKIDKDKFRNHNLVDIKSN
                     KEDGFVCDKIEAVFKTNDKKDLVESLAYYTEIEELEEGKVLVRYTGERALSRNPHRSY
                     NCAFSLAKDLEKIKKLNDKGVIIRDILNSYFTDDNHGKKLGIYKEDLDMGVSTICIMS
                     IFLEKNELNMKIDFRYPKGISWEFITNRINEIGKKENLIVDIYKDLKLLYVKPDSELI
                     NKLSSAYKQGFGKEAELFTKGAASYARVLKNGVAFGPTIEGDNPNSHQANENISIDTL
                     YKAIEVYIYALYSLAFQ"
     gene            complement(47008..47499)
                     /locus_tag="KRL67_000321"
     CDS             complement(47008..47499)
                     /locus_tag="KRL67_000321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HBH1521228.1"
                     /translation="MFNIFKKKENKIEKIYSPFKGNLVNLEDVPDAVFAQKMMGDGVA
                     IIPSEGKLYSPVDGEIVNVFDTKHAIMIKSNDNTNILIHVGLETMSLEGKPFDVKVKA
                     GDKVKAKDLIMEVDLDFIMSNDLNIITPVVIMDDADSVKRTVKDRIEPGNVDINSIIM
                     EVE"
     gene            complement(47525..48337)
                     /locus_tag="KRL67_000322"
     CDS             complement(47525..48337)
                     /locus_tag="KRL67_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HBH1521229.1"
                     /translation="MRYKLICTDMDGTLMGKGFEVSEENVKALKEAMEKGIKVALVTG
                     RPYNAMKYFTSVLGDDIYIISTNGTYFKLLGYEYKKVLSKEALKKIYTIGEKYNLNKH
                     FKGCKIVISNNEIGQEHPYRLINSKNKEEDRIEIIENASCETLLEKADNEILKCILFS
                     ENVDSLREAKEEFKKQEDLEVVSSGKINFEVMSKGTSKGIAVKKFCDILGIDSKEVIC
                     IGDNENDISMIKFAGLGIAMGNATDEVKSMADFVTDTNVNDGVAKALRKILS"
     gene            complement(48350..49501)
                     /locus_tag="KRL67_000323"
     CDS             complement(48350..49501)
                     /locus_tag="KRL67_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HBH1521230.1"
                     /translation="MINFYQPSRINFGQGTLSELPRIIGKYGKKCLLVTTPNVEPLDK
                     LYKRVTLNLIEKGIEVVHFDRVEPNPTVELINEGFELAKKESVDVVLAVGGGSSIDTA
                     KVLALTFGLEKIDWDYMFSTYTNPFKIYDKVSEKELPLIAVSTTSGTGSQVTQAAVIS
                     RGSEKNTIFHQNCFSKECIVDPELMLTLPDRITASTAFDAFTHAFESYINPNANDFTE
                     IMSEKSMELVINYLPKVMEDKNNIEYRTKLAVADTLGGSSLANSGAAAPHPLSEIIGG
                     VTHIPHGEALAIVFPKFAEKFYKDNIKKFAKVARMLNQELEKVEDDEAAKTLGNEIES
                     FLKRVGLKTKMSEFNVTKEQLEQITSCPVLGFLPFGKKEDLVEILEKSY"
     gene            49771..50412
                     /locus_tag="KRL67_000324"
     CDS             49771..50412
                     /locus_tag="KRL67_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HBH1521231.1"
                     /translation="MKYLIDSANLDEIQKCLKYGFKGITANPSMYLKEKVNFYEFIET
                     CTNLNPEILTAEVIGNNLYELINSCDKILNINKNTIIKINFSEVGLELINILNSKGVK
                     TACTLIFNVNQASLAINAGADYLFPFIGRNDENGNDGIKNLTEICNLISINGYNTKVV
                     AASIKNVFHLTQASLCGCDYAAVTYDLFKKASFNQLTVDGANTFERDWSKVNK"
     gene            50438..51982
                     /locus_tag="KRL67_000325"
     CDS             50438..51982
                     /locus_tag="KRL67_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HBH1521232.1"
                     /translation="MNKRLLDIAELLLNSSDYITVDTIAEELKVSNKTIRNDLVILDE
                     WLLEFNLSLDKKTGSGVIILGNEDVKLKVIRDINDKSNCIYAYSPEDRKRYILSKLFI
                     KNSKFRIRDICNELHVSRATVHKDLVVIQNFLQNFKVTLVRKTNNGVYLEGKEKDIRK
                     AIFELATVNKSYTELKEILFSKSNECKDTPSTKIFKELFNYDFEKLSQITLHTLQVEK
                     SNLSDEYYINFLIHIAICIKRISIKRYINLSEGFFRELLEHEYFEKSKILCANLSEAF
                     DVEFIDEEVCYILLHIEGLLKSIKKTTTLEPLKNEEITEYNLSEAIAVHWGSILNLNL
                     KDDLILIKSLANHLKSVLHRINYGFTITNPILNDIKRTFPYTYKAAKESNTVIKQLMN
                     YEVNDDEIGYLALYLISAIDRSKSPLNTILICHCSDGVSNLLVQKLSFEFNQINIVKS
                     IPLSSISFTNFDDVDLILTTAPVDFEHNAELININALLSKNDISRLSTIIKKLYSEKN
                     KILSYK"
     gene            complement(52165..52791)
                     /locus_tag="KRL67_000326"
     CDS             complement(52165..52791)
                     /locus_tag="KRL67_000326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HBH1521233.1"
                     /translation="MKKEMIKDYLLITISAFLMALAVNFFFAEHTLAPGGITGLSVVL
                     STFLSMPVENISLGISMPLLVMGLLFLGKGFGIKTLYITLMGPLFLKVIPQTHITDNL
                     LVAGVIGGLLVGTAIGIAIIRGCSTGGTDLVAMLINKVIKFLKLPVILFMLDGFIIVA
                     SGIISKNFMISIYSLISLLLIIKTIGFVTTKFGKNTEEINNVNSACAQ"
     gene            complement(52926..53642)
                     /locus_tag="KRL67_000327"
     CDS             complement(52926..53642)
                     /locus_tag="KRL67_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentose-5-phosphate 3-epimerase"
                     /protein_id="HBH1521234.1"
                     /translation="MKHIKIAAGLAHVNYGHISAVVKEAMDAGVDYVHSDAADMHDLK
                     NMQLMGGHQIIEAIRPVTDKPIECHIYTRDCDRLFIEKIAAAGCNLLIIPAEHFLGAP
                     LAYIINYCREFGMKVGLTIGCYTPLCFVDEAIYDIDRLQIVVHGVDETDGKDNWGWRK
                     SAIDLVKRARKMIDEKNPKCELAIDGGLRADNMEPLIECNPDVVILSSAIFKDKDGIT
                     AGVKNCRKAIDEAATKFGLE"
     gene            54068..55282
                     /locus_tag="KRL67_000328"
     CDS             54068..55282
                     /locus_tag="KRL67_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HBH1521235.1"
                     /translation="MRNCIIAQSGGPTAVINASAVGIYEKNLETKYFDKVYFGINGIE
                     GILNKNIIDSDSLNKDNVLNLKQTPSSGLGSCRYKLADYKINSYDYDRVINILDEYEI
                     DTLFYIGGNDSMDTVMKFSQYCKNTTCKIKFIGIPKTIDNDLLGTDNCPGFGSAAKLI
                     STLIMETYLDFSIYKNNGIFIVETMGRDTGWLAASSSLARLNDTPLVDLIYLPEIDFY
                     EDKFIEDVRKIFKEKNKAYIVASEGIRDKYGNFICEQKTSAHDKFGHSQLGGVSNYLK
                     NLIVSSSITTRVKTLELGVIQRCAMHLASDTDLKQAAEVGAYGIELSKKGYTGVMAII
                     NRTCNNPYKYEVSHTDISKIANKTKYFPKEWINEECNYITDEALNYLYPLIQGKPDIK
                     YKNGLPYFSIIY"
BASE COUNT        18103 a        10053 c         6129 g        21526 t
ORIGIN      
        1 gagtaattca tagttattta tcaattaaaa agagatattg cataattatt caatacctct
       61 ctttgtatat ttacattatc catatctagt tatctatttc aacccaatat tagactacct
      121 actaataaat ttattctcta catcattaat aataattttg cactaaataa catcattagc
      181 ctatctctga tgcattttct ttaattttat ttatagtttt aatcaattta tttctctctt
      241 caatatgatt ttcaaataaa tcaattatac ttccaacagt tctaaatggg tcttccatca
      301 acactttgtt atcctctata aatccattct tgactacata atcgactata agtttaacaa
      361 aatgtatttg cttagtatta aaactcttat tatttaaaaa ctctgaaaaa gcttcatttg
      421 cagcttctct atccaatccc actagttttc tcactaattg acttactgga gtatttccaa
      481 actccttctt ataatcatca caattaccta actgctttaa gagtatttcc tccaacgttt
      541 caacatcttg ctttgttatc tttttattat ttcttaattt aaatattgct aattcatctt
      601 tatgactatt taaataatac tctaccttct ttttataatt tttaagattg tttgcattat
      661 atacagaatc atttctatct tctgctacaa tcatatcttc aaaacttgta taatatatct
      721 tttgactaga cttttcaaga tacttaataa gctctcttaa tgatatacgt acttcttcta
      781 cctcaaatat atctgaatct tcccaaaaat cctctgtcat tgctttttca atagtatatt
      841 tttgttgctg aatctgtggt atagtcccaa gttttgaaag tttttctgct gtagccatta
      901 catgccttat acctttactt gcattattat tttgtaagta tgcaagttgt atattataca
      961 taactacatc aaatctttta gccaattcat catcactata tgaagttatg agtggtgata
     1021 tatattctct tatctcattt tgtgctactg ctccaatatt actccaaaca ttttcatttt
     1081 tatacttatg tacataactt aaattcatcc taactatata actatcctca tttaaagctt
     1141 ttacagaagt tataagttct tctaacaatt cttttctaag ttctgcatac tcaccttcta
     1201 tatatttaat atcttgaagt tctttaacca tacttacctt tatattaaat aatttttcag
     1261 taagcgtttc tgctttattt ccctcaaatc ctttaggatt aaatttaaaa aattcaaaat
     1321 tattacaaaa atcaaatatt aagaatcttt ccttatccat atcaacacct aataaatctg
     1381 gacataatct agttcctcta cctatcattt gccaaaactt agtcttactt ctcactttct
     1441 tgaaaaaaac aagatttagt atttcaggta tgtcaattcc tgtatcgagc atgtccactg
     1501 aaactgctat ttgaggtaat ttattcttat cagaaaaatc atctattata gaatctacat
     1561 aatttatact atagtcaact gcttttacaa agttcccacc atactttgga tacagcttat
     1621 taaatctttc aactattgcc ctagcatgct ttgtattctt tgcaaaaatt atagtcttac
     1681 caatcttttc ttctccttct attttcaacc cctctgtcat tagcttattt aaaactaaat
     1741 ctattgtgtc agaattaaat agccactcat ttactgcatt attccctatt tcgtctccaa
     1801 tactctcatc atcattaaat gtattttcat attcttcctt ctcttcatca gaaagctcat
     1861 catacttaat tccatcttcc ataatcttag ttttaaactc aatactagta taatctacta
     1921 aatatccatc ctcgactgct ttattatact cataagcata tgtaggtact ccattttcca
     1981 tgtcaaaaac tgaataagta tttttatcta tctcatcctt tggtgtagca gtaagaccca
     2041 ttaagtaaga atcaaaataa tcaaagattg acttatactt tttataaata cttctatgcg
     2101 attcatctat tataattaaa tcaaaatgac caggtgtaaa caatctcttt ccatccttag
     2161 acttcgtatc atcaattgca ttcatcattg ttggatatgt agaaaatatc atcctagcat
     2221 cttctgggct gtctttgcta tccaataaat tacataacgc caagttaggt aatagcttag
     2281 taaaactttt tttagcttgc tttaccaatg cttttctatc tgctagaaat aatatatttt
     2341 taatccaatt atgtcttgaa agtacatcaa ctatagatat agcaattctt gtttttcctg
     2401 taccagtagc acataccaaa agagtttttc gttgcttatt ttcaagagcc tcacatacag
     2461 ccctaatagc ttctttttga taatatctat tggatatttc atcatttatt gttacagaag
     2521 ttaaagactt cttactcttt cttctatcta tcataagttg taattctgat tttttataaa
     2581 atccacaaac tttcctatct gaatagtcat cccaaatata catatcaaaa ccattagtca
     2641 aaaatattac tggtctttga ctatatctct tttccaaaca atctgcatat aatttagctt
     2701 gttgttgacc gattttcggg tctttactcg ttcttttcgc ctcaactact gctaatggct
     2761 taccattttc accataaaga acataatcta catatcctac accactttca ttaggcatac
     2821 cttgtacttc tatttcttcc cctatatctt ttccaaattc ccaacctgca agctttaatt
     2881 ctatatttat atatcttttt cttgtctcaa attcgcttac ttcgtctatc ttgaaatcat
     2941 agtttacagt attaactttt ctcttttcag ttagtgactt tcttaattcc tcattttctt
     3001 ttattacttc ttcaagcttt ttatcttttg aacttaatct ctcataaaga tttttaagtt
     3061 cgtctggtct cgtccttttc tcttccccat gaagtaataa atcttcatta aagtcttctg
     3121 ctgtatattc atctgaataa caatagtcta tccaactaac aaattgatgc aaattgttaa
     3181 gtgaaagtat tgcttctcct ctctctatgt tcgcactagt atgtacagat aaattaccta
     3241 atttaactat atattttaat aaaggtaaca tatcatagtc tataagttct ataaaactat
     3301 tgttatgaat caacgtacta agattatctt gataaggcaa tactaaatca ctatcattag
     3361 catatagcca ttttactgca agctctaaag ctcttcttgt tatagttgca caagtagatg
     3421 ggctaactag tatacttttt tctgcttcta agcaagcagt attaaaacta ttgaaatttt
     3481 tcttcttttg taaaaaatca aaattaccca aggcttttct cccccttttg tcccttagtt
     3541 aaaataaatc tctctaaaaa ctatttctta cagctgtttc ttatactaac aaatacttct
     3601 tcattttctg taaacaattc gtacatatct tcaaactgtg gtatatcata tccatgagaa
     3661 tcatcatttc tcaattttct aagtgtttct ataactatag aagaattttt aggtaataaa
     3721 ttgtgatact cacaagctaa aaggtggtca taaaagtttt tagattttat gtttaaatca
     3781 attgatttat aatacaacat tgcttttgtt aactctagaa atgagaccat atagctttta
     3841 aaaccttctt ttatatatct agcccataac tcaggcattt cttccttatc atataagatt
     3901 ttgaagtttt gcagatcata caagcaagaa ccaaaatccc tatttacttc ttctattgag
     3961 ttccaagata atttgtttct aattaccata catgtccctc caatctaaga tatctattac
     4021 atcactgttt tcaaaataga atttatttat ataatcctta aaatctttag attcataaat
     4081 aacttcacct tctaaaatag cttttttaag tgctattgta taattatctg taacaatttt
     4141 taggtctaca tcaatgtcca atatatttct tagagcttct gtcttagttg caagttctgt
     4201 aattggtact tttttgatgg gaatccaagc tatgtctata tcactttctg atgtatgttt
     4261 atctgttcca taactaccaa atattatgat tgctagagta ttttcttttg gaaatacatt
     4321 tattattgag tttttaatct catttgatat atttctcatt taatcacctc ttgtttatta
     4381 ggtaatattg cttaattgaa tttatatcca taatctttta atctatttat acatatttct
     4441 ctttcttcat cagtaaacaa ctctttatac ttttctttta atactaatac ttctaatgat
     4501 aaatctaatc tcttacactc ccatagcctt gtaaatcctt cagtagctcc ttctttattg
     4561 attaattctt tagcagctaa taaggctcct ttgttattta tcagttgtaa aaatcttgtt
     4621 ggcttatagt tacattgtaa atcagcttct ttatagatat ttaccatgtc atgatataaa
     4681 tctaactcta tttgattcat agattttatt cccccatttt aataattgta ttttagttga
     4741 ataattctcc tttaaaagct ttttgcatta atgagttaaa gttgtcttct agttctttta
     4801 gacttgtttt tattcctact ttcaatttgt ctacttgatt aacaaactct tcaaacttat
     4861 tttgaagttc tattgatggt actggtatta atattttttg aagttctttg cttctaattc
     4921 cttgtgcaga gctaccagtt ttatatttat ctatttctac cctaaaagct tttgtttgca
     4981 tatattgtgc taaaaaagca ttatttaaaa tttttttatc tccctgaatt gttataatcc
     5041 tttgacccat agcaaacttt tttaaatcct ttggtatcct acatacattt ccaaatgtat
     5101 gaccttctgt aacaaaaagt acatctccag ctactggaaa acctcttttc atccaagaat
     5161 tataggtgtt ttcagatata tattcattaa tatatttaaa tacaccattc cctacagatt
     5221 ttgcatttat tattctaatt ccattctctg aaagaattgg agttttacca cgataatcaa
     5281 taaaatttgt acaatctttc aataatacaa acttttgttc cttaaaattt ccaaacattt
     5341 caataaatct tgatttaact aattcatcca gtacttctat ttgctctttt cttttatcta
     5401 ttaactcttg agctttatct aaaacttctg atatatcttt ttgagcttcc atttcaggta
     5461 aatctacttt taaatttttt aaatcattaa ctgaaaaact tgcttgatta actgattttt
     5521 ttattatcct tggtaactgt cttctaaatt cattactatt aaaaaaataa tttaaatact
     5581 ttggataaca aacttcttta tctggtcgta aacataaaag attcattcca tgtataattt
     5641 ttttatttat atcttcatat attgcacttt tccctaaatg tttttcacta ttaatatgtg
     5701 acattagtat atctccattt ttcaataaat agtcttctaa ttttcctaac tcttttatgt
     5761 cagcatatcc cattttatta gtatcaatat aagagtttgc tatagtttct attcttgtaa
     5821 ttggatatcc aaattttgta tcaaattgtt taatactagc cccatttcta ataaattcaa
     5881 aaacttcatt caatctatat tccatactct acacctcaag cattctttct aattcctcta
     5941 atccatctac gatttccttc tctaactccc taactctacc tataataaca ctaggagcat
     6001 cataaactac ttcttcatac actacctctt tatacttatt aatagataaa tcatacccat
     6061 tctctcttat ctcttccaca tcaacaaaaa aactttgctc tgttcttttt ctatctactt
     6121 ccccatcaag attatcaaat ctttcaataa tatcctctat atcactatcc tttatctcat
     6181 ttctcttatc atccaaagaa taaccatcag atttcatatc atagaaccat accttatcag
     6241 ttccaccagt tcctgtttta gtaaatatca ttatagctgt agatactcct gcataaggct
     6301 taaacactcc acttggcatt gatattatag cttccaattt atgattatca actatttctt
     6361 ttcttatcga tttatgtcca cctgttgaac caaacaatac tccatctgga actatacatg
     6421 cgcatcttcc accaattttt aaaactctta aaaaaagtgc caagaataat aactctgttt
     6481 tctttgtctt agtcactttt aataaatctg ctgacactgc ttcataatct aaacttccct
     6541 taaaaggagg atttgctaac accaatgtaa acttatcttt atctgtatta acctccgata
     6601 aagaatcctt atactctata ttaggattgt caacgccatg caacatcata ttcatagcac
     6661 ctattctaag catagttcta tccatatcaa atccatagaa cataccatta ttaaaatgct
     6721 ccttcagccc ttgcactaag aaaagacttg aatgattatc ccttaagtat tgttgtgatg
     6781 atactaaaaa acctgctgaa cctgctgccg ggtctactat aatatcttct ggtgttggtt
     6841 taataagtct tactatcata tctattatgt gtcttggagt cctaaattga ccattagtac
     6901 ctgctgtagc cactttggat aataaatact catacaaatc tccctttgta tctctatcct
     6961 tacttaattc taaattacta ataccatcaa ctattttaga taacataaga ggtgtaggaa
     7021 ttttaaatat tgcatctccc atgtactttg cataagctga ttctttattt ccatgtaatt
     7081 tttttatgaa agggaacact tcattttgta ctatttcata cattttttct gagtttcctt
     7141 cattagaaaa cttactccat cttaaatgtt gatgttcatc atcaaatatc ttttcaaaag
     7201 aaacacccaa aagagttgct tctgcttcat ttttggtttc tacttcatcc aatcctttaa
     7261 taaataaaag atatgtaaat tgttctataa cttctaatgg attagttaat ccaccagtcc
     7321 aaaatgtctc ccaaatcttg tctactttgc tccttaattc tcctgtaatc atatatgact
     7381 ttccctccag tgttcataaa ataatttaaa tatatatttt agtcgtacgc cttaatagtt
     7441 agtgtcttat gttaaatcca agtttttact acacagttat taaattattt ttcctattta
     7501 catattttag ctaaaaaact aatgcaatac tcttatttta taattattat atataccata
     7561 caaaattatt ttatcatata tccatcatag aagtcatcta cctggataac tcttaccata
     7621 ttataacttt ttaatattaa tttaaactta gaaagaaatt ttttattttc ggaatgatga
     7681 aaatatattt aatttcatac aaaaaagagt gcctcctgaa ctaaaaagtt cattttgaga
     7741 cacctcttaa tttaatctta cttattcttt ccctttatta ataacacttc actatctcta
     7801 ttgcttttcc tacacatcca caaacttcca tcctactctt aacaaaatct ttaactgact
     7861 ctaaaccttt tttaccatat ttcttagggt caattacctc tgggtcagaa ttaagcacat
     7921 ccttaacccc attactataa gcaatcctta attctgttgc ataattaacc ttgcaaatcc
     7981 ctctttttat agattcctta ataacttcat ctggtattcc agacccacca tgtaatacta
     8041 atggaactga tacaacctct ctaatttcag ataatctatc taaatcaagc tttggctctc
     8101 ccttatatag cccatgtgcc gtacctattg ctactgccaa agatgatacc ccagttctct
     8161 ctacaaactc ttttgcttct aatgggtctg tatatgctcc accatcacca ccatctaaat
     8221 cgtcttcctt acctccaact ttacctaatt cagcttcaac aggtatatct gaaggcatac
     8281 atgcatctac aacagattta gatattgaaa tattatcttc gtaactttca tgagaaccat
     8341 caatcataat agaagtatac ccaactctaa gtgcctgcat agctagtcca aagcttgaac
     8401 catggtctaa gtgcattgcc actggaacac ttgccttctc tgatgctacc tttacattcg
     8461 ctaaataata atctagtcct gcatacttta ttgttgaagg tgttgtttgc aatattactg
     8521 gtgatttaag ttcttcagca gcctctatta ctgccataac catctccata ttttcaacat
     8581 taaatgcacc tactgcataa tgcccttctt gtgcatctaa taatatttgt ttagttgtaa
     8641 ctaatgccat aatttctcct cctatatgtt tatactgatt tgatttcttc gttatttaat
     8701 tttgatacta tttctctact tgttttgcta tttaataact catttaaaaa ttcttcattc
     8761 attaattttg aagatagttg agataatgtt tttaagtgta aattatcacc attatctgta
     8821 gctgctatca taaatataat atgtgccaag cttccatctt ctgattcaaa gtcaaaccct
     8881 tcttttgata cacctactgc tacacgtggt acctttactg catctgtttt tgcatgtggt
     8941 atcgctatcc ccataccaaa acctgtagat gatatttctt ctcttttaag gatttcttct
     9001 ttaaacttct ttttatctaa aagaacacca gatttatcaa gtattccaat catttcatca
     9061 attacatctg ttttattttt tcccttaaca tctaaaatta ctgtttcttc gctatataaa
     9121 gatatattat taaatacgtt ttccgattct gcacttttat ttttatgttc atctttagtt
     9181 gcatttgtat tttctatagt atttttctca tttactactt taggtgtaac catcatataa
     9241 agtacagcac ctactaatga acctacaaat atacacccta cccataaaag tggcttattg
     9301 actaatccta gaaccaaaaa ccctccatgt ggagctggta cttctatttt cattatgtaa
     9361 gttaaaattg atgatattga tgatgcaatc ataagtactg gcaacacttt tagtggattt
     9421 tttgctgcaa aaggtattgc cccctctgtt atatgagtac tacctagtat ataattgatt
     9481 agtcctgctg ctctatcttc ttctgtaaat ctatttttaa ataaagtaga tgctattgct
     9541 attaccaatg gtggtgttat acaagctgca gatacacctg ccatgaataa ataatttccc
     9601 tgtcctagaa gcatagttcc agttacatat gctgctttat ttactggacc tcccatatca
     9661 aatgctgaca tacatcctat tactattccc aaaattatag ggcttgaatt ttgtaaccct
     9721 gatagaaagt tcatcatagc attgttaagt gctgaacatg gcccgccaag taacaacatt
     9781 gctacaccca caagacctac gctgacaata ggaattataa aaatcgattt aagaccttct
     9841 aattgtcttg gtaattttct caacaaatgt acaaatagtt tgactaagta acctgccaaa
     9901 aatcctgata tgattccacc taaaaacccc gaacctgtat cgaatgatat cattcctcca
     9961 acaagacctg ccaacatacc agctcttccc gatacagatt gagctataaa tgctgccaat
    10021 acaggaacca tcaagttcat agaatatcca ccaacagatt ttaacgtagc tgctatttga
    10081 ttatattgtt ctgaagttgg gtcaaatgaa taaatccccc ataaaaaaga tatagcagta
    10141 agtactccac ccgccactac taaaggtaac atgtgagata caccattcat taagtcttta
    10201 tacaactttt gaccaaacga taaatttcct tcttctgcaa ttttactgct tgataagttt
    10261 ccttttctaa tagctacttt tccatcaata catctttgta taagttcatt agctttatgt
    10321 ataccttctt tgacaggtac ttctattact ggctttccat tgaatctgtc catatcaaca
    10381 tttttatcac aagcaactat tattgcatct gccatttcga tatcttttgc tgttaactta
    10441 ttttctacac ctatagctcc attcgtttca acttttatgt cacaccctaa ttccctggct
    10501 gaatttttta atgcttcttc tgccataaaa gtatgagcta ttccagttgg acaacctgtt
    10561 acccctagaa tcattttctt tcccattttt ataccacctt tctgactctt atatttttta
    10621 tataatcttc cacattctcc aaattaccta taccattact ttcagccaca cttgctcctg
    10681 ttgctgctga cctttttaat gcttcctcaa tatgttctga attttccaac catatactta
    10741 aaaaagcagc taatgcagaa tcaccagcac atgcagagct gattactgta actggttgag
    10801 cactagcata ataaattgat tttccattat aaaaataaga acctttttct cctaaagtta
    10861 gcaatatatt ttgagcacct tttaaatgca gtaatttaag tacatcctta atatcttctt
    10921 catctcttac aataattcca aatatatctt tgatttcttc atcatttggt tttatcaaat
    10981 atggattata ctgtagtaat tcctttaatt ttggtgaact tatatcgagt attgtcttta
    11041 tatttttatt tttacaaatc ttaaatatat cctcatagta attatcatca ataccaggag
    11101 gtagacttcc acttattgat aaataactta tatcttctaa tgactgtatc ttctctagca
    11161 tatctagttt ttgtttttcg tttacatatg acccagaatt tacaaattta aattcatcac
    11221 ttccatcatt taaaaatata tttatacgag ttgtatcttc tacccaagta ggtaatacgt
    11281 caaatcctct cttttcgctt tcttcaacta tatattttcc tgaaaatcct ccaaaaaatc
    11341 ctaatatctt agattctata ccataatgac ctagtacaaa agttacattt aatccctttc
    11401 cattaggaga atatacagca tttgatgtac gatttactaa ttttctcttt atcccattag
    11461 tagatatatt catatctatt gcaggattcg tagttaatgt atatatcatt gtcttccccc
    11521 ttgcttgtgc tgtgtatgtt ttcattataa taaaaaagtc tatgaaaatc ttttcacaaa
    11581 tagaaaatcg attttcatag acttttcaat ttaattttca gaattattat atctgaccat
    11641 ataatctata attatcctag atataatata caagggcaat ctggaggata cttcatattc
    11701 agaaattgat atatgttcat gtttgtatcc tacaatatat aaatccgaat actttataag
    11761 ttttgcactt tcactacaac agcaagttat aactttagct ccaactaaat tggcattctg
    11821 tgctgattcc acaagctcta tggtatttcc attcaatgaa aatataatta taagttcatc
    11881 cttttttatt gatttagatt ttattttcat tatgttgggg tcatttataa aaacaacatt
    11941 aaaatcaagt aattgcaatt taagactaaa ctcttttgct acatattcag taagccctct
    12001 tgctagaata tatatcttac tagatttatt tatagttttt attatattga ttagtgtagt
    12061 taaagatatt tgttctataa ctctctgtgc ttctataaag gatttattta ttatatcccc
    12121 catattttgt atatcatcat tttcatcttt tactgttaat ctatacctaa gttcattgaa
    12181 tccatttaaa ccacattttc taattgctct tgaaacggtt gctggagatg aatatgtttc
    12241 atgagcaatc tctactatag ataattctgg taatattgat tcattttcat ttataaattt
    12301 aattatttca agttccgtct tcgttagatt ttcagaatta cttttgttta gctcaattat
    12361 catatgttat ctccccttat ataagtttta tttgtctaaa tttatcgagt aatataatat
    12421 attacctctt aactatttat ttttatatac aattaagatt tagtcaatgt ctatatatgt
    12481 aaatttataa tatcataatt ttttgcctaa agtttattat gttattttat agcattttaa
    12541 aataaaaaag aattgtacct cttccaaaat ttggatgaag tacaatagat attcctcata
    12601 ctgatatgtt tatgattaaa agtataaata taaactttgc tataagtcat ataattaata
    12661 aatcatttta tttacaatta taacccccct tataaaacta tattaataat atatacagta
    12721 tttataaggg gggttattta tatttaaaaa ctatttactt ttctcttaat tcttctccat
    12781 tggttcttat tacattttga taccaataaa aactatcttt tttacaacgt tctttacttc
    12841 catttccatt gttatctata tctacatata taaatccgta tcttttttcc atttcttgag
    12901 atgaacaact tactatatct attggtgacc acatacaata agctaatatt tcagttccat
    12961 cagctatagc ttcacctatt tgctctatat gtttttctaa ataatctact ctataactat
    13021 catgaacttt tccatcttca aatttatcat gcataccaaa tccattttca gctataatta
    13081 ttggcttttg gtatctatca taatattgag ataaagtatt atataatcct tttgggtcaa
    13141 ttgcccaccc ccaaggattt gcttttaaat atggattctt tatattatca gttggattca
    13201 tagtgttttt acttgctgat actgttgtag aataatagta tgataaagct agaaaatcca
    13261 tagtattttc ttttaagatt ttatcatcac cttcctccat aactatattt atgttatttt
    13321 ctttgaaaaa tctcttaata tagactggat actctcccct aaaacttaca tcagtgaaga
    13381 aatattgtag cctatttctt tttaaagcaa atactacatt ttcagggtca caatcttctg
    13441 gataagatgt acaatctgcc atcatcatac ctatcatcat atctgaattt atttcttttc
    13501 caagcttctt agtcaaagct gtagctatga attgatgatg aatagcttga tatctagcct
    13561 cttctacatt atctacctgg tctacacaaa atgcaactga attaaatgat tcataatgta
    13621 ttaaatttat ttgatttata actatccaat atttaacttt atctttgtat ctatttagta
    13681 ttaactctgc atatctaaca aagaaatcta ccacttttct attagaccat cctttatatt
    13741 ttaatactaa attcaaagga gtctcataat gaagtagtgt tacaataggt tccataccat
    13801 tatctattac ttcatttata agattatcat aaaactttaa tccttcttca ttaggctcta
    13861 aatcatctcc atttggaaat attctagccc aatttattga aaatctaaaa cactttaacc
    13921 ctagctcttt caaaagcttt aaatcttctt tgtatgtatg ataaaagtct attccactac
    13981 gcttaggata atatccatta ttatctttca tagcaaactt tattctttct atagtcatat
    14041 cagactcata aggtttagat atatctctat ctttatcata catatgtata tcagctaaac
    14101 accatccttt gccaccttca ttccatgcac cttctaattg attagctgct acagctccat
    14161 cccacaaaaa acctttagga aacttaccca atttaatcac tccttatttt atttatttaa
    14221 atcatttact attatttacc ttatattcta tagaccttac tattcctact aaagacatag
    14281 ttatatttaa ctcgctgaat atggtcataa gagtatcttg tgcatgtgca aataacagcg
    14341 aatgtgctct tttctctcca ccagcttccc cttgtataat cttggtttga gctctatgtg
    14401 cttctgtcat aaactctttt gctttagcta gttttaaatc agcatttcta taatcaaact
    14461 tttccaaatc gttaagagca tgtttaattt ctgttctagc ctcacctgca cgaagtatta
    14521 tttgcattgc cacctctact aattcttcat ctattatatg ttcttcaatt gtcatattta
    14581 taatttcctt tcaatttcta aatttaaatc tcttgactct ctaattttag ctcttctttc
    14641 tcataagctt taaaaaatgg ataccatatc ataaatagta atactatatt tattgctaaa
    14701 acaataagac ctctaacatc ttgatttacc ataaaagtag atataccaaa gggaacatac
    14761 cataaattaa acaccttact tggaattgta acaaatccca tactcattga taaataagta
    14821 gttattgata ttactattgt acctatccac ataggtaaca ttaaaaatgg gttccatgct
    14881 actggagctc caaatattac tggttcattt atattcatta tagaagggac aatacatgct
    14941 cttcctattg cttttattct tccaactctt gataaacaca tcattataac taatggtaat
    15001 gtagcaccca accctcctaa agttatccaa ccagtttgaa taacttcgta aacatatata
    15061 ttagttgcta ctcctcctga tgctactgct gctgcattag ctgcaattgc accaagttga
    15121 attgaataag ttataggaga tagtacccat ccacttattc ctaaagaata tataaatact
    15181 actaagaaat ttaagagtac aaatccaggt aatgtctgtg ctatatctga tactggttta
    15241 aatactctaa ctattatttc aaacatatca aagttaagtc cataaattaa tccccaacct
    15301 acaaacatta tcactgttat aggtaacata ttatcaaacc acttgcttac aaaatctgga
    15361 agtgctgaat cttcgccgaa aaatgaaaaa gaaccaaata aagacattat taatccagaa
    15421 aataatcctg caactagtga tacaagcatt ccttctcctc caaacctgtt gaagttaaat
    15481 atagctcctt cattcgtaat ctctggacga atcatcatta acatcaatcc agcacctgca
    15541 agaccagcta ctacactcat actttcaaat ttcttattcc tcattatttc cataggcaat
    15601 aaaaatgcta taaataatcc taaaagtcca aatgtatatt gatttattgg acttaaatca
    15661 ggtaaaaagc ttatgtaatt cttcaagaca gaaactatag ttactaatga gcctacaaac
    15721 accattggca aaactttcat tatagttgat tgaactgatt gtacccatat attcttagtc
    15781 atattattta caacaggagt aaatttttca cttaaaaaat ctataaattt attcataaac
    15841 actctcccca taaatatttt atttgtttga tattaattct agtgcttctt ccaaagcctt
    15901 tgcaccatct agtcttccat atgcagtttt agatatacat gctactggaa cgttatattc
    15961 attagccttt tgttgaattt cttccttcat ataatctaaa tgtggtccta gtaataaaca
    16021 atctatttct gataaatact tatctatctc tgactcactt ctcgctttta tatctgcttc
    16081 tatccccttt ttctttgcac tctttctcat gctagtcgcc ataaaacctg tacttgctcc
    16141 tgaaccacat gccaaaagca ctcttatatt tttcataatg atacctccag tatttttatc
    16201 cattaattta tttcttaatg aactttttat tttattatgt ttatattgta cttaccaaat
    16261 aagtttattt cacataaata tatttcctaa taataggaaa tcatttactc ttaattaaac
    16321 atattaatat agtataatgt atcttataat acgtacattc aattatataa ttatcatatc
    16381 atccagattt tattttaaaa ctatgcgagg gaatctaatg aaaacatcta aaaaagaact
    16441 atttttaaat ttcataaaaa cacagcatga ttggatagat tcatctacat tggctaatta
    16501 tcttaatgta tcaacgcgtt caattagaaa atatgtaaac gaaattaatt ccaatggtga
    16561 atttatattg tcctctaaaa aaggatataa agtaaatctc aataataatt gtcaaaccaa
    16621 aattgatagt tcagagaaca tatcacctga taacagatta aatttaattt taaaagaact
    16681 tatagttaat agtaatggaa ttaatatatt tgatttaagt gaagaattat ttgttagccc
    16741 agctactatt gaaggggata tagtcaaagc taataaattt ataggaagtt ataatttaaa
    16801 aataaagcaa tcgaaatttt tattgaaact agttggaaat gagtctgcaa aacgcaaatt
    16861 aatgagctct ataatattta aagaaacggg aagtgatttt ttaagcttat ttgatgtaca
    16921 aaaaatatat cgagagtata atttgactaa actaaaagaa aatataatat atatattaaa
    16981 aaagtacaat ttatttataa atgagtatgc tataaacaac atacttcttc atttaatgat
    17041 aactatagat agaataaaaa agaataatta catagagtct gttgaagtag taaactacat
    17101 agacaataac atagatataa atattgcaaa agacatatca aattttttag aatctgagta
    17161 caatataact ttaactagtg cagaattata ctacttagta tttcagttaa caaacaaaac
    17221 aacagtctta aactacaatc aaatggatac taagtcattg tcaaattata tagatgaaca
    17281 ctttgttaaa ttaactaaaa aaataattaa aaatgtatat gacttatatc ttatagattt
    17341 atctgatgag gaatttgttg taaaatttac tcttcatgta aaaaatttaa tcagtagggc
    17401 caaaaataat caagttttaa gaaatcaaat accccaaaag ttaaaagatt cctacccttt
    17461 gatatatgat atatctgttt atatttgcaa tcagattcaa actttagaaa acgtagatat
    17521 agacgaagat gaaatatcgt acatatctct tcatgtaggt tcattctttg atagacaaaa
    17581 attattagaa tataaagttc tatgtgcatt aattactcca aattactatg atttacaatt
    17641 caaaatagta agagaccttg agaagagatt taatgaatcc atagaaatta ttcagatttt
    17701 ttcagatact cataatctgg attttgacaa taaggtagac atggttatta ctactttacc
    17761 aattagcgat agatgcccta taccttttgt ttatgtaaat ctttacctaa acagaaaaga
    17821 ttatgataat atccaaagta aatttattca gattaaagat agaaaaaata tacttactgt
    17881 acaaaatcat ttagaaatgt attttagtga atctttgttt atgaaaaaca tatatttaga
    17941 tagtgctaaa gattatataa aatttatggg aaatattctg tgcaaaaata aatatgtcaa
    18001 acccaattat atagatgatg ttcttataag agaaaaaatg tcttcaactg catttaataa
    18061 caatgtagca attcctcata gtatgaaaat ggatgcttta aaaactggag tatgtttaat
    18121 tgtcaacgat aaaccagtta aatggggaga ggaaaaagtt cagattatag ctatgatacc
    18181 tataaatgaa aaggaaaaag aaaaattcaa ttacatattt gaaagcttta ttgaaatatt
    18241 atctgagtgg aataatatta aagaacttac taaagcagat aactatagta gttttatgaa
    18301 tagaatagct tatcttattc aaaatatcta aatccattaa tttgtttcca atatatgttt
    18361 cttaagttat taacacaaaa tagacaagct gtcacactaa aaagtctaaa ttaatatgtg
    18421 acagcttttt acctaatata ccttgtttta atactacata ttttttgact gttgtatact
    18481 agttataaga ttgaatattt gtgttgttgt agctgtcata ttctcactag cagttgcttt
    18541 atccattgct tcttctattg tcatagctct atttactata gaaaaataag catctatacc
    18601 ttcttcatta catttaactg catcttctgt tgtggctcca gccaaaccta ttacttttac
    18661 cccatatttt tttgccagtt ttgcaactcc tattggagcc ttgcccatag cagtttgatt
    18721 gtccaatcta ccttcaccag ttatgacaaa atctgcatct tttaactctt cctctaattt
    18781 aatttcttct aatattattt ttattccaga ttctaatttt gaatttaaaa atgctaaaaa
    18841 agcaaaacct aatcctcctg ctgctcctgt tccttctaca ttagctacat ctttacttaa
    18901 gtaattttta actactttag aaaaatttct aagcccatta tctaactcct caattatttc
    18961 tgatgttgca cctttttgag acccatatat gtatgcagct ccattttttc caaacagtgg
    19021 attatttaca tcacaggcta ttttaaactt acattcatct aattccttta atctattttc
    19081 tatttttata tgtcttattt catttaaaac ttttccacct aatcctacta acttgttatt
    19141 ttcatcataa aactcaaatc ctaaggcttg aagcatacct accccagcat cattagtagc
    19201 acttccacct attccaacta taaagtttct atatccttta tttattgctt cctttattat
    19261 ttccccaaca ccataagttg tagtgtataa aggatttcta agttcagtcg gaactagtgt
    19321 taatcctgat gcttgagcta tttctattat tgctgtactt gtatctttta ataatccata
    19381 tgttgcatcc acttttttag ctattggtcc tgtaacttct atagtctctt tcttaccatt
    19441 cattccttga actaaagcat ctactgtccc ttcaccacca tcagcaagtg gcaatatctt
    19501 tacttgcaca tcttctttaa ccttcaatat cccctttttt attgcatttc ctgcctctat
    19561 tgaagataag cttcctttca aagaatctat agaaattaat attttcacat ttatctactc
    19621 ctttttattt actccttatt ttaaacatat aattttataa gcttaattta tcttttattt
    19681 tatactctac atctctcttt gtatacaact tataccatta atattctttt atcttataaa
    19741 aaagggagtg tctcaaaata aactatttag ttcatgagac actctttttg tataaactca
    19801 aatatatgct taccatttca acaatgataa ggaggattat cgctatttta agacaattac
    19861 tttttagcac tatctaatca tttttagcac tatttaacca atatagtttt aacttggttc
    19921 attaagcact ctacatttac agagttatta tctattgata taaattctaa ttctttctca
    19981 tttaggttaa tccatttagc tattttaatg tttttgttaa atactatgtt ggctgatttt
    20041 gtttcttctt tagaattata aaatctaact actaatgcct cttctttttc agcctttttt
    20101 ataacactta acacaaggtc cttattagtt tccttaagca aactatatga ataatctact
    20161 tctatttctg attgattcaa tttcatagca ttatatggca ttttattata agtttgtatt
    20221 tttgttaaat actctttagc catatttgca acatttgctt ctagtgtaga tttttcatga
    20281 gtaactattg ctaaatctat ttttatatct cctatcattt gagaatctgg agtaggcaac
    20341 tttattcctg atggacgacc tgggcgtctt accatctctt ctttacccag gaatcctaca
    20401 cttctaaata atgttatggc tattgtgtca aatttttctc ctactatttc aaactctctc
    20461 gtactgtttg taagaacagc tactccatgc tctttgtcag acaatccaac gaatgttagc
    20521 attggatata tagaatctgg tctttcactc cattcttcct gttgccaaac ttccatagca
    20581 tcatcataca cacttctctt tatatgtcca aactgattat cagacacaga aaactttgaa
    20641 gctatatttg ttggtatata agttctaacc ctacaatctc ttgcttggtt atttatgttg
    20701 aatactacat ctattatagc tttatcattt ggaacattta taactatatc aaagtcaata
    20761 aatgaatctt ctctattatt ttttctagct tgtatatctt ttggtacttt taatctataa
    20821 tttatattta tctcattatt aaatctgttt ttctttatag tgtgatttgc tactatatta
    20881 tcgttaaata ttaatttctc atttggaagt ggtgaaaaat catactcatc tccttcatct
    20941 cctccatttt ccatcaataa tacattttca aaagttttat ttaatttttt atcaaatatc
    21001 tttaatgtcc cgttttcatt aacttgaata gtataaaagt cattatctac catgtcacat
    21061 acttcataag ctttttctaa catatattca acttctttta tcatatacgc cttatacccc
    21121 atggcaggta ttttatcttt aaagtttatt gtatatacca taaatgaatc ataatttcca
    21181 taatgaacaa tttgacggtc tattaatcct gcatctattt cttctttatg taccacttca
    21241 aaatccaact tattattatc tctatcaaat atttcaaatg ctttcatttt agttataact
    21301 tgggcgctta ctatttcatt cctatcgtaa ggcattaagt taaatgctac taacttatct
    21361 aaagccattt cacaactcat tgcatcaact attcttcttt tataaaatat tattaattgg
    21421 tctacttttt cctcagctaa gaaaaatcta ttcattattt cctggtgaac tttatctgaa
    21481 caacaacatc caatactgtc atgagcatga tttttcatta tttctttcca aattaactct
    21541 attaatccat gatgatattc aaatcctaga gaatatgcaa tagaagctaa tggctccaat
    21601 atgttagtta tctttgattc tattctagca tttgctgatt ttatatccat tcttgttgag
    21661 aatatacttc tatgaaccct catatatttc ccatctaaaa attccccttt tattgtatct
    21721 atatcctcat ttttttctag ctctttatat atgttttcat atctacttaa gaaaaatttt
    21781 ctctctggat aacattcttt taatttatat ataacttcaa atatattctt ttgaattggc
    21841 atttggtcat gcccatttgg aagtattata tggtcagtag ttgcaccctt atcaagaact
    21901 ggtaaatatt tatccattct tgttcttagc tcatcttcat tcattggtag atattttcct
    21961 attgcataac ctaatggaag tatttgaacc aaaacagaag acccatcatc actcttccaa
    22021 ttaaactctg ttttattagt tccttttctt tcagaacatc ctctccaaaa cattgagtat
    22081 tttatatcaa acccatttag tatttgtggc atttgagcac tttgaccaaa tgagtctggc
    22141 aaatatccta ttttcatata ttctccaaat tcatcacaat cttttattcc atacagtaaa
    22201 tttctaagta tcgattctcc accaactacc atttcatcag tttgagtata ccaaggacct
    22261 attattagcc ttccttcttg aactaatttt tttactctct ctttacattc aggcttaact
    22321 gctaaataat cttctaatat agcagtttga ccatccatca cataatatgg atagttatga
    22381 ttattttcta acatatccat tatttcttcc atattattta caagtaatat tctagattcc
    22441 tcagttgaga agtaccattc tctatcccaa tgcatatgag gaactatgtg tacatttttt
    22501 ttcattgctt atcctccaaa acttatctga taacattttc tgtctaatta aaaatccaat
    22561 aaaaatacta ctgagtttac acaatttttt aatttttatt ttataatata atcaaatctt
    22621 tgatttaaat ctctaataat gtatcttcta atatatcttt ctttgatttt tttgtttcct
    22681 agttattatt aataacattg ctgatattat agctccaagt actgctgcaa agaaccatat
    22741 tgatgctgcc ataaatcctg acatacttcc ctctaaaagt gccaatgaga atataccagc
    22801 tccaggtact tgaagtccta tattgaaaaa tgctactata gacccagtta tggctgaacc
    22861 agctaccaat gatggtataa cacgaattgg gtcctctatc ataaatggta tagcaccttc
    22921 agttattcca gccaatccta atatccaagt ttgctttcct atctcctttt ctgattctga
    22981 aaataaatct ctgcctaaaa tagtagctat tgtaactcca aaagctgaaa ccatcttaac
    23041 tgatgcaaat attgcataag gtataaaatt cccacttgct attgctccta tcgcaaatgt
    23101 ataggctgct ttatttattg gtccacctag gtcaaacgaa accatagctc ctattattgc
    23161 acctagtagt attgcattta ctccagtcat tccatttagc caatttataa gaccattgtt
    23221 aagtgctgct acaggctttc ctactacaaa taacatcaat aaacctacta ctaaagaacc
    23281 aactactgga tatacccaaa aacttacgaa tccagctaat gtacctttgg ctggtatatt
    23341 cttctttaaa aattttacta tataaccagc aagtaaacct cctaacatac ctccaagaaa
    23401 tccactgcct attaaatcag ctgctagacc tgctgcaaat cctggtccaa gacctggttt
    23461 ttcagctagt gaataagaca tatatgctga taatactgga accatcaatg tacctaacat
    23521 accactagaa agctttctta attgccataa ccaagaacct tcttgattgt ataggtcttg
    23581 tagaccaaat gcctgagatg ctaatactgc aaatgcaagt atcatacctc ctgccacaat
    23641 aagtggtatt atatgagata ctcctgtaag tattgctgac ttcacatctt ttttccatga
    23701 tgatttttca caatatgata tatcagatga tttttctata taatcacctt taccttgttt
    23761 ttgtgctact tctaaagctt cgtatattat ttcttttgcc ctttttagtg gagctgctac
    23821 tgacgttttt acgtttggca aatgttcaaa tctttctgta tttttaggtg ctacatctgt
    23881 tgcatatatt attgcatctg ccctttttat tatatctggt gtatgagggt ctaccatacc
    23941 attagctcct tgcttttcta catacacatc cacccctatt tctttaccag ctttaattaa
    24001 tgcttctgct gacatatatg tatgtgctat acctgcaggg catgctgtta ttgctaatac
    24061 aatttttttt gattcatttt gaatcctaat ttcttctttt atttgttctt tcttttcttt
    24121 tatatctaag ttattaaata actctttctt agtttttgaa tttaacaaca tattcatata
    24181 atcttcattt gacagtctag taactaactc agaaagtatt tgtatgtgtg tatttccgcc
    24241 ttcactttct ggtactgcta ttaaaaatat tagctctact ttattattgg ggtctacgct
    24301 ttcccaatcc tctattggta tctctaaggt catagctgca aatgcagctt ctttaactga
    24361 cttagatttt ccatgaggaa tcgcaagccc tttttcaaaa cctgttggag ataaagtttc
    24421 tctgtacatt acagcttcta aatattcctc ataagatgtt aacttgcctt catcatatag
    24481 ctttcttgct aagaacttta ttgcgtcttc ctttgttttt attttttgtt ttaaaaaaac
    24541 aaggttttca tttgtgattt tcttaaattc caaaattacg ccctccttaa atcatttaat
    24601 gttatgaata tagcataaaa taaatcatga aaatgttttt ttgaaattat acacacaatt
    24661 tcttcttaaa aaataaagat ttgtgtattt atacacaaat ctctatctta accagtattg
    24721 ttctattatt gctctcataa tcatcataat tggaacctta tctgatatat tgtacccatt
    24781 tacttgttta attgttgaat agcaatataa acttatatct gcaatttgtt gcaattgatt
    24841 ttcacatata tgtgttagag ctactacctt acatccctta tcaactgcta tctttgctgc
    24901 ttctatcact tctttcgttt ctccacttaa actaagggca aaaaacatat cttgttcacc
    24961 taattttcct atatcatgaa gtacttcatg tctttgacta tagtaagtta tattattatg
    25021 gctacctcta atttctttta ctaatatctc acataaagga atattttgac ctattccata
    25081 aactataata ttcctagatt tttgaattat atctgctact ttatttattt tttcataatc
    25141 tattagctct aaggtctttt ttatatctga ttcaaaacca tattttgact ctttattctt
    25201 attttttaac tcttctttta aattattttt tagttgagag aaaccatcat actccatttt
    25261 tttacatagc ctaactatgg tatttggcac agtaaaaaat ttatctgcta aagactgtat
    25321 tgataatttt actatttctt ctttgttttt tctcatatat tcaattatca tgtcttctgt
    25381 atctgtaaac ttatactcat atttttttgc tctttcataa aattccatag tttctcctcc
    25441 atctatttca ttatttataa gtaaaattca aaaatactat ctaaaatttt cgttaatact
    25501 attataattt aatactttta actttttaat gcatactact ccttttatta tacataaaaa
    25561 atgtgtactt cctatatcaa tagagatgat tcaagttttt attttgatac atctccatca
    25621 atattctttt agaaaataca catttttttc aaatacttat ttttaattaa cactttatgt
    25681 aatctattct ttatatataa ttattagcct tgaaaatatc tttaaaatca accaactcaa
    25741 tattatttct ttttaaccac attttaactt ccttatcaca taacattgca tgttcatata
    25801 ctctctcttg aacaagagag gatgatttta taagactata atctataaat cctggatggg
    25861 taaccatcaa tgttatctct ttatctaaat ttatatattt ttcttcaaat aatttaacaa
    25921 aagatttatt taacttgtaa aaatcattct gaggatattt acttggcata ctaatacatg
    25981 aatctaaata atagggacaa cttggtatac cgtattcttt cgcagcatca cacattgctg
    26041 atattgatac atcttcttct ataccatgta tatcaatata ctcaggcaac ttattcatat
    26101 acaacttgta agtttcaatt tgagcacaaa cttcttcata tacttcttca taattaaaaa
    26161 cctctttacc tgtcagtagt tgcgttcttc ttattttgct agaaacaaat tttccacctt
    26221 catctaacaa attctttatc ttaatatgtt ctttgcaaca aggttctcct attactaaat
    26281 taacatgtaa accaaaacat gcatcatcat tttttaccat ttctatagct tgtcttgaaa
    26341 agggcatatt caccatgatt ccagcacttg taacaatacc gttcttataa gaagatatta
    26401 tccctagtgt aacaccttct gagtatccta tatcatctgc cctaactact aatttcttca
    26461 ttttatgcat ctgcctgctt tctcaactca tcaagttgca tttccatctc atcctttact
    26521 ttaacaacat ctgttcctat gatgatttga actgatgtat tactcaattt aattacccct
    26581 tttgctcctg tttttttaat attatcatca ttcaaaatag tagcatcctt cacttctaaa
    26641 cgtaatctcg tcatacaatt attaagagtc actatatttt cataaccacc gcaattttta
    26701 attatctgct tagccatata tgaaaaattg ccatgagaaa gttttaaatt cttttctttt
    26761 tcactaactt tatcacctat ctctatctca acttcacgtc ctggtgtttg tacattatct
    26821 tttattatta tatatttaaa tgttacataa tataatacaa agaatacaat tcctataggt
    26881 ataactaacc acgctttatc tccataacta aaacttaaga catagtcaat tatacttgct
    26941 ccattaaatg aacccaagcg agcacccagt aaatatagta caactccacc tagaccagaa
    27001 taaaaagaat gaattatata taactttgga gatgcaaata aaaacgaaaa ttctataggt
    27061 tcagttactg ttgccataaa agaggttaat gctccagcag tcattgctcc ttttacttct
    27121 tgcttctttt tatcctgtgc tgttttatat atagcataag aaatagctgg tattgagaac
    27181 atcataatta tgtaaaatcc tgtccagaag aatcctgcat ttgggtctcc tgccataaat
    27241 ctaggaattt cccctcttat aacttctcct gttgatgttg tatactcacc catttcaaat
    27301 aatatgtaag catttaaaac ttgatgtaat ccagttggta ttagtgcacg attacagaat
    27361 aaaaataaga atattcctat tgcaccagct cctcccaacc aaaaaccaaa ttggttcatc
    27421 gcagcttgtg gatatatcca taataaacta aatacatatg ataaaattaa agcaactact
    27481 ggtgataatg taattacaca tttttctccc gcaaagaaac ttaaaaatgc tggaacttta
    27541 actttatatg ttctattgta agtccatgct atacaagctc caacaattat accaccaaat
    27601 acacctgcat ttaatgtatc atttaataac tctaaatttg acttaaatat catatatgca
    27661 actacggctg caaccattac acttgtcttg tctttagact ttgagtaggc actagctgct
    27721 cctaataaaa ataagtagtc cccatttgaa aatactatat tagatatatt agatatagct
    27781 ggcacattaa gcatatttgg accaaataat aatcctaata tccctgctgc tggcattaca
    27841 gctattggca ccagcatagt ctttcctaaa agtgacagtt gttgcatcat tttgtttaag
    27901 aacctcttca tatccaatcc cctttctatt tcggtttaat tttttgtgaa aacgatttgt
    27961 tcacatttta tattttaaat attatatttg tttaaacaaa ttgtcaatat tttttattta
    28021 ttttattttt atcatttagt ttcacatcta aatcctttca agtatattgc tatatctagt
    28081 ctattttttt aatttatttt tttcggtttt aaaatatgtt taaccatata tttgaagcaa
    28141 cccacaaaat aattcaattt tatccttttt atggtttata tgattctatt ttcactatac
    28201 atgtctaaac acatttgata taatttgttc atatcagttc aataatatta ctaaagacct
    28261 ttaaggaggg gtaggggtat atggctaaaa ataaattttc agagatatat gaagtattaa
    28321 aagaagaaat acttgatggt aaatatacaa gtaatatgat gttgcctact gaacttcaac
    28381 ttattgaaag gttttcatgt agcagaaaca ctgtacgtag agcaatttct caattaaatg
    28441 cagaaggata tgttcaaagt ataaaaggca agggtgttgt agtgttagaa aatagttgtt
    28501 ctaatgattt ttttctaaat atgcacaatt ttaaaggtgt agaatcgatt gtagaagata
    28561 aaaaggttaa tactgctact agtgttctcc atttcagcaa aatattaatt gataataagt
    28621 tatctaaaaa gacaggtttt aaggttggta gtgaagtata ttaccttcat agactaagat
    28681 atattgataa catccctaag atactcgaca taaactattt tctatgtagt atagttaaaa
    28741 accttgatgt ttctatagct caaggctcta tttataaata tatagaagag tctctaggta
    28801 caaaaattgt ttcatccaga aagatattta aaattgaaaa agctacggag cttgagttaa
    28861 aaacactacc tctaaatgat tataactgtg ttggtgttat taaaaatagt gtatatactg
    28921 atgatggaaa gctttttgaa tatactgaat caaagcatac acctgaaaca tttgtattta
    28981 tggatgttac tcaaagatat taaaaaatta tgtttaatta aacataattt ttttacgttt
    29041 agacacttta tcccatacta acctcactat tttatatata attatatgtt tatacaaata
    29101 attatttatt ttatatttta gcttcaatat tagtttaata cttgacaatt tgtttaaaca
    29161 tattataatt aacttataaa ttacaggagg tatcaagtat gaaaaattgg tggaaaaaag
    29221 cgacggtcta tcaaatatat ccaaaaagtt tcaaagatag caacaatgat ggtataggtg
    29281 atataaatgg tattatagaa aagttagatt atctttcttc tttaggtgtt gatttattat
    29341 ggttgactcc tatgtacgtt tcccctcaaa gagataacgg atatgatata gaagattatt
    29401 ataatataga cccaaaatat gggacaatga gtgactttga aaaactctta aaagaagctc
    29461 ataaaagaga cataaaaata atgatggata tggtcctaaa ccatacatct actgaacata
    29521 aatggtttaa agaatctaaa aaaagcaaag acaatccata tcgtgattat tatttctgga
    29581 aagatgctaa acctgatggt tctgtaccta ataactggat atccagattt agtggaactg
    29641 cttggaaata tgatgaaact actaaccaat attatttaca tttatttgag gaaactcaag
    29701 ctgatttaaa ttgggaaaat gaaaaagttc gtgaagaatg ttacaaaata ctagaatttt
    29761 gggctgataa aggtattgat ggttttcgtt tagatgtagt caacctacta tccaaaacac
    29821 ctggattacc tgatgaccct ataacaggac caaaaggaga tggaagaacg cactacgcgg
    29881 atggtcctcg tatacacgaa tatttacata atatgaatca aaaagttttc aagcctaaaa
    29941 atatagttac tgtaggtgaa atgtcatcta ctacacctga agagtgtatc aactatacta
    30001 gagaaaatcg agaagaacta tctatggttt ttagcttcca tcatatgaaa acagattata
    30061 aaggaggcgc aaaatggact aatgaaatgt tctctttgga taaattaaaa aaagcacaat
    30121 ctgactttca atataaaatg tatgaaggaa aaggatggaa tgctctattt tactctaatc
    30181 acgaccaacc tagagcttta tctagatttg gagatgatag attttttcgt caagaaagcg
    30241 ctaagatgtt agccataaca ctatttggtc tacagggtac tacatacata tatcaaggtg
    30301 aagaaatagg tatgacaaat gcctacttta ctaaaataga tgaatatgat gatgctgaaa
    30361 gtaagaatgc atactatcat atgattaaat ctggtattga tgaaaaggag gcattgctta
    30421 tacttcaaca gaaatccaga gataatgccc gcactcctat gcaatgggat aatactattt
    30481 ataaggggtt ttctaatcat aaaccgtggt taaaagttaa taatgacaat attagtgtag
    30541 aaaatgagct taatgattct cgttcagtat tttatactta tcaaagatta atcaaatatc
    30601 gtaaacaata tgatattttt acagaaggta cttatagatt gttagatgaa aatcataaaa
    30661 atctatttgt ttatgaaaga gagtataaaa aacaaaacct tcttgttata agtaatttta
    30721 ctcatgataa tgtagtatat aaattgcctg aaacttattt gaataaatta aactatacaa
    30781 tacttgaaac caattacact agagatataa ttgaaaatga aatggaaata aaaccttatg
    30841 aaagtataat gatatattat aaataaagtt atttatgtaa aaatcggtat aacatccagg
    30901 tgttataccg attttactaa attcaaaata atatttatag aatctctata gattacaata
    30961 attatttgac ttctacatca ttatattttt ttgaatatac tttagtcata tagaaatata
    31021 taggaactac aattatcatt ccacctaatc cccaaactat cttcataggt tcatcgatac
    31081 ctgcttttat cagtaataat atacttataa taactgcaaa tattggtata attgctcctc
    31141 caggtattct aaaatttaca tctttaccag catacttctt tctcaatatt agtacagcta
    31201 aacatgtagg aatatattga ataaatctcg atacaacact tatttgagat agtgttgtaa
    31261 atgaacccga ccatgctatt agtagagttc ctatgacaga tactataatt gctatatatg
    31321 gagctccatt tttattagtt tttcttatag acctaggcat tagcccttgt tcaaccaacg
    31381 cagcagcaga ccttggtgta aatatggatg atgcaatatt tatacctcca acagatacta
    31441 tagttcctat tgtaattata tatccaccta taggacctaa catcttaatg gcagcatctg
    31501 caacaggagt agctgaccct gctaagccat tacccaacac ccctatactt attcccagta
    31561 taagcatata tattactgat actacaaaca ttaccattac caatgcttta ggtacatttt
    31621 tcttagggtt ttccatatct tttgctgcaa cagctaaaga ttcaaatcct gtaaaagcat
    31681 aaaataaagt tatagatact gctacaaaag ctgatgacgc actttgacct ttttgtatta
    31741 ttacaaatgg ggtaaaatta gaacccttta taaagaataa tcctacagct ataaataaaa
    31801 ctattggtaa taattttcct atagtcatga tattatttaa gtgcttagat tgtttcattc
    31861 ccataaaatt tataatccca agtcctacaa ctaatatacc aattattata tttttagtaa
    31921 gaggttctgc tgcttgtggc catacagatg atagtgttac agcaaagaaa tttgccatag
    31981 tagcccaagc aatcatacta actatccact tcataaatcc aacttcaaat ccaataaaat
    32041 ctccaaatgc ctcttttgca taaagatatg gacctccatc agtatcaaac caactacctg
    32101 cttcagcaaa acataaagca atagaaacag ctaaaagcat acaaaaaact aatactaata
    32161 tacttgctgg acctatatct gtataagcat caccaggtaa cccgaatata cctgacccta
    32221 ttattgcatt gattcctaat agtacaatac tccaaaaacc taacttaatg cctttttcca
    32281 ttgttattcc ccccaaagcc aacttaatat atttatattt ttcatttaga tgcttcttta
    32341 actattgcca taaatatttt tttcatatta tcataatctc tagtcatcat ttctggatgc
    32401 cattgtatcc ctattacaaa ctcacttcct tccttttcta tagcttcaat taaaccgtct
    32461 ttagatgtag ctgaaacaat atatcccggt gctactttgt tgacaactaa gtgatgaaaa
    32521 ctattaacta aaacttcttt ttctcctaat atttcatata attttgtccc ctcctttatc
    32581 tgtactgtat gtgtaggtac gttagataga tgttgctgat tgtgtttaat ataagcacct
    32641 tcaattaaag ataaatcttg atacaaacta cctccttcag ttacatttat aatttgttct
    32701 cctctacata tacctaaaac tggttttttc atatctaaag catgttttaa taatttcata
    32761 tcaaaactat ctctctttgg tgatattgcc cccaacttat tatgaggttc ttctttccat
    32821 atgagtggat ttacatcttg tcccccagat agtataagcg catctatatt agacacttgc
    32881 tcctttataa tctcatcatc atatacaatt ggcacaatat atggtatagc ttcacacata
    32941 actactgatt gtatatagtc attatttaca taagcccttt catagccagg aaacatgccc
    33001 ccttcatcaa ttaataaatt acctgatatt cctattactg gatatttctt tttaaccact
    33061 ttcttcaccc cttaacttat ttgtgaacaa taaaaaatgc tacttctctt aaagagcaag
    33121 tagcatacca agattttatt tatttgaaca gtataattta ttttagaaat actttttttg
    33181 aatactagta atataaataa ctttatttac ttatatttta ttttaatcct gtttagtttg
    33241 acttttttaa attgttttaa gtacaactaa taggtaaaat aggcatttag attttccttt
    33301 ttatcaacct attaaatgct gtttaatata tttttccccc ttctatttaa ttcacttccc
    33361 tttctaccag attcacatat tatcaaatcc agctctttaa gtatgttaag tattcccctt
    33421 acctcacctt ctgttatact gtagcctaat tcatttatta aattaacaat accatttcta
    33481 cctatatttt tattaagatt attggcattt tttaaacact ccattactaa ctttactttc
    33541 tctatagatg ttctatcatt taatattctt atttctttac taaaatcttc agttatattt
    33601 aataagttat gaggtaaatc atttattaaa ataacatcct cagaactcat aatgttaata
    33661 tacatagcag tattttttaa ttctcttata ttacctggcc attcatatgt attaataata
    33721 tcttgaacat cttgactaat tgctctcttc cttttcataa aatgctttag cataacttct
    33781 atatcttctt ttcgctctct taaaggtggt atatttatgg gcaatacatt tatcctataa
    33841 tataaatctg ctctaaatct atctcctttt atcatttcta ataagttttt atttgtagct
    33901 gcaattactc taacatctat atctattaca ttctctcctc caactctcat tacttgattc
    33961 tcttgtaata ctctaagtaa tttgctttgt aagtatatag gcatgtctcc tatttcatcc
    34021 aaaaatatag ttccattgtt agcctgttca aataatcctt ttttacctcc ttttaaagct
    34081 cctgtaaaag caccttcttc atatccaaaa agttcacttt caagtaaatt ttctggcata
    34141 gctgcacagt ttatcgctat aaatggctga ctatgtctcg gtgatgcatt atgaatagac
    34201 tgagccatga gttcttttcc tgttccactt tctcccgtta taagaattgt ataatcagat
    34261 tcagctattt tcatacctaa tttcttagtt ctttccatct tcgttgagac tgtttttata
    34321 tcatcaaaag tatatctagc aacttgtccc ttttctctaa gcttctttga taggttttgc
    34381 tccaattttc ttatgtaagt tatttcttga atacaaaaat aatatccagt aacccttcca
    34441 aaactctcta tgttatactt atttacgttt aagtacttat tattaaattt tataacttca
    34501 tcatgtgcct tactcaaact aaaaatcttt ttaatattct cattctctaa ttcatctata
    34561 tttttcccta taatattact ttgaatatct agtatatcac ttaaactatt attgcaaata
    34621 attatgtttc ccttatctga taccattgcc atacctaagt ttgataaatt tattatagaa
    34681 tttaactcat ctgcttttat agtaagttct ttgtatttag tatttattcc tgaatataaa
    34741 ctaattattt catctgtata tttaattagc ctctcagtaa tttctttatc atccagtttg
    34801 agttttgcta ttatttgaat aaacgttgaa ctatcaatat acctatgacc taagtcaata
    34861 acttctttta tatattttgg aactaaactt gcctcccctg gtgttatagc taccttaata
    34921 tttctatatt cttcattggg attgtatggt ataaaattca aatgatttat tcctatattg
    34981 tataaattag atattgtttc caatacagta tgcttattat cgtttactac taatacatct
    35041 attccttctg gcaatgaaaa taacttatat atatcctttt gctttatact cctatttata
    35101 gttattatct ttgagacatc ttctacatat ttttttattt ttaatactct atcatctatc
    35161 attaccaata ttatgtcatc ttttatcaat tccttctctg ttattttatt taaataataa
    35221 ttttttatta ttactttatc tttaaatatc atttctagat ttttatttaa gaaacattct
    35281 aatttagaca acccatcagt tactactgct attgttttca ttttattact ccttttaaaa
    35341 tatactctac tcttttataa tatacatttg attttcactt taacagcaca aaaaattgta
    35401 catttagtat actaacatat tcctttatta gttttatgtc cctaaatatc atagttgcaa
    35461 tatctatcat atttcttaaa cttccataaa gcaaaaattg tgccaaatta ataattttct
    35521 tacttttatt ctaaagttcc tcaatagtat ttacagactt agcacttata acttataagt
    35581 ctttatatta ttaatctatt tttataggtt aattgtacaa atcatatatc acttttaccc
    35641 attaaattca ttataaaaaa gatggaataa cgattcgtca ctccaccttt tttgtaataa
    35701 aaaattgtat atataaacac tatttgctac tcttctaagc acaaacagta tctacatact
    35761 ataacctttc accattagat tctattactt gtttatacca gtcaaaactc ttcttcttaa
    35821 tcctagacaa gtctccactt ccatcatcat acttatccac gtatataaac ccatatctct
    35881 tggccatctc accagtagat gcacttacta aatctataca tccccatgga gtatatccaa
    35941 ataaatcaac accatcttct acagcttctt tcatttgctc tatatgtgct tttaaatact
    36001 ctatacgata gtcatcattt atcactccat tagattcttt tttatcataa gctcctaaac
    36061 cattttcaac taccatcata ggtattctat atctatcata aatttcattt agtgcatatc
    36121 taagcccttc agggtctatt tgccatcccc attcactaga cttaagatat ggatttttca
    36181 ctccacctaa tatgttgcca cttgtattat cttctttaga atcactactt acacatgtag
    36241 acatataata actaaatgta taaaaatcta cacaaccttc ttttaaaatt tctaaatctc
    36301 ctttttccat ttttatttca atattatttt ctccaaaata tctattcata taagttggat
    36361 attcgccacg cacttgtaca tctccacaga aataattcat tactctacta tctctttgag
    36421 ttttaactgc atcatctgga ttacaagtat aaggatacat tgttgcaaat atttgcatac
    36481 atccaacttg ataatttgaa tctgtttcat gagctaattt aacagcctta gcacttgcta
    36541 caaattgatg atgtaaccct tgaaaacgaa gttgaggtct atctacttgg tttataaaat
    36601 ctgtagtccc ttcatttaat attccttgtg ataagtagcc tcccataggc attgtagctg
    36661 aatttatttc attaaatgtc agccaatatt ttactttgcc tttgtatctt ttaaatattg
    36721 tttcacagta attcatatac aagtctatta catctcttga aacccatcca ttgtatttct
    36781 ctgttattgc aaatggtatt tcatagtgag atattgttat taatggttct atattatatt
    36841 ttaaacattc atctattaca ttttcataaa atttaagtcc tgcttcattt ggttctttct
    36901 ccattccagt tggaaatatt ctagtccatg ctattgaaaa tctaaatact ttaaatccca
    36961 tttctgcaaa taaagctata tcttctttat atctgtgata aaaatctatt gcttcatgac
    37021 ttggatagaa tgttcctttt tctataactc tcgttattct tttacttact ttttgagaac
    37081 ctccagtaca catatctgaa caagaaggac ctttcccatc taaattccat gctccttcac
    37141 attgatttgc tgctgttgct ccaccccata aaaaatcttt tctaagtgcc ataactctac
    37201 ctccaataat ttatttatag taaatttatt ttaaaataaa aatacatata tctaattttt
    37261 aattaataaa actctttttt acgctaaaac agttatcaat tcttgactat tatttacatt
    37321 cttttgtgta ttttctataa tgtccacata gtcagagtaa tttgtaacaa ctactggtgt
    37381 ttctaggcaa tacccttctt ttgatatgct ttctatatca aaagttatta aggtctgacc
    37441 ttttttaatc ttatcccctt gattaacaaa agatttgaaa tacttacctt ctaattgaac
    37501 agtatttaat ccaacatgta tcaatagttc acaaccatta tcagatataa ttcctatcgc
    37561 atgttttgtt ggaaataaag tcattacagt cccatcaaat ggtgctacca cttttccttc
    37621 tttgggtgtt attgctacac cttttcccaa tacacctgtt gaaaatgctg catcttgcat
    37681 ttgttccaaa acctttacgt aacctcttat tgggctatat attacttctc ttttgttttt
    37741 ttctacaaca ccttgcccat tgtcatcttt agaaatttct actatagcat catctttcca
    37801 aagaaataat gttaaaacga atcctactac aatggctact attattgcta ttatagttgc
    37861 atatacaccc gtcatatctc cagttgttgg atttatataa ttaggtatac caaatattcc
    37921 aagtccacct attacataac tttttacatt cattattccc attacagctc ctgccacagc
    37981 accacctaca caactatata tgaatggtgt ttttcttggt aaagttattc catatattgc
    38041 aggttctgtt acaccacata tccctgaaat aattgcaggt atacacagct cttttatttt
    38101 cttatctttt aatttaaagt acatcgctgc cactactgct gtttgtgcaa aagatggtac
    38161 aaatgctccc actaatattg tgtcaaatcc catagtcata aggtttgaca tagctattgg
    38221 tactagaccc caatgtaatc caaatattac aagcacctgc cacaataatc cagttaatgc
    38281 gccacataaa attggtgaaa agttaaataa agctaaaaat ccatttccaa ctatgttaga
    38341 agcataggta gctataggcc ctataaccat aaatcccaat ggtaaagaaa ctaataaaac
    38401 tgccattggt acaaagaaat tctttactac atccggtatt attttagcaa acactttctc
    38461 taacttagct gctaatagta ctattaaaat tactggtact actgttgaag tatagttcat
    38521 tgctattact ggtaatccta agaaagttat atatactggt gattcaaata ttgttccagt
    38581 aaatagattg tataaaggtt gacctgtaga tgctagagta tctaattgaa tacctgaata
    38641 gcataaaact gcacctataa gcattccaat aaatggtttt aatttgaatt tcttagcagc
    38701 tgtatatcca agaattatgg gcatatactg gaacattgca tctccaattg catttaacat
    38761 aatatatgca ccatcagttt ctccatacaa gcccaatgct gaaaatactg cattgaatcc
    38821 ttttatcata cctgcagcac acaaaactcc tagtattggt tggaaaactc ctgatattat
    38881 gtctataaac ttattaagta atccttttgg tgcatctgag tctgaattat ccactgtatc
    38941 ccctcctaat ccagccaaat cgcatacaac cgcataaaca tctggaacat ggttaccaat
    39001 aactacttga tattgacctg cacttttcat tacagtaact ataccatcca ttttctttag
    39061 cacatcatca tttgctattg attcatcttt taacttaaaa cgtagtctcg ttatacagtg
    39121 agataaactg ctaatattat cctttcctcc tacatttgca ataatttctt ttgatagttg
    39181 ttcatacttt ctcatttttt ctcctccatt ttctttttga aatatgaagg tttggccaac
    39241 ttaatgtcac cacccaatat atataaacct aattattagg atttatatct gacttaatac
    39301 ttttgcttac tactctttca acatgaatag ttaagtataa cttttcctca tctgttaaat
    39361 cgtaattgta ttttttatgt ataaattcat ctaacttttc tatgcattta taagcatttt
    39421 tatatttgac ttttattaca tttaataaat catcctcatt actttcatgt aaattattac
    39481 taacaattct ttctgcaaaa tactttaaat gagtgataaa tctatgataa taaactgaat
    39541 cttcatcaaa tactattcta taatggtact taaccacatt tgcgatttct tgtattattt
    39601 gtgtaatttc atatataatt tttatacttt gatccatatg tgcattaact atatgtaatg
    39661 ctataaatcc agcttcatct tctgacagtc taatttcaaa ttttttttct atataatcta
    39721 aagcctctaa acctatttta aactcatttt tataaaatct ttttatatcc caaagtagta
    39781 cattttttac atttactcct tcacgagctc tttctattgc ggtatacata tggtctgtta
    39841 aagaaatgta aatactatca tttaatcttt tccctaattt ttctttagca tataaaatta
    39901 tttcatctga caattccata tatctcatag gaatatctgc tattaattct ttaaatttat
    39961 cagatatatt tgggtctgaa agtttgaata tcttatcaat tttatccttt gatatatggt
    40021 ctcctgtacg ttttttataa gctaaacctc ttcccataac tactatttcc ttatgatttt
    40081 catcagttgt aataattaca ttattattga gtattttttc aataatcatt gtgatgcccc
    40141 ctttattagc attaagttaa aataaatctt ttttaattat ttagaatatt taaaatgtaa
    40201 tttattacat aaaaaaaaac ctatttatta cacattaaat agactcccat gagtcctgag
    40261 taataaatag gtttagcttg tatttaaaca atcactatcc gtttgtattt tattttaatt
    40321 attatttatt atatgttagc aaatttattt tttagtgtca actttttctc aatattttaa
    40381 ttttaaataa attatataaa tttaatctac aaagtactat ttttatcaat aaacaaaata
    40441 aaaaaggtat tactttcttt aagtaatacc catttatttt taatttaagt ggttcttctt
    40501 aatctcatca agtagtccat cttctaaata aatttctaat gcacatccta ccattgcctt
    40561 tactgctttg tgaagtttat cataagcttc accagaattt gcatagttta gaaattcttt
    40621 ttcatgaaca tataccattg gacttatatc taaggctatt tctgtataca tagtagggca
    40681 aacttgactt acattaccta tatctgtaga acccactgga ccatctttac cttctaatat
    40741 atctgttata cctacctcaa tcaaattatt ctttgttatc ttttgaagta ctttgttatt
    40801 tattatgttg tcaaaagaat tttcaaagta tctataagaa agcttagctc ctgtcattaa
    40861 ctctgcacct tttgcacagt ttataacttt tttagttact tcatctaaat aacttctttc
    40921 agcagctctt acgaaaaact tacaagctgc tttttctgga actatgtttg gagcctctcc
    40981 tccatttgaa actataccat gtattctaac atctgatttt acatgttgtc ttagtgcatt
    41041 tatcccagca aaagtaagtt gtactgcatc tagtgcattg actcccatat gaggatgact
    41101 agctgcatgt gctgatacac cttcaaaact aaactctaat gaatctatcg ccaaagcctt
    41161 acagtttata ttattaaaag cttctatatg catctgataa cttacatcaa catcatcaaa
    41221 agctcctgcc ttaactaaat cacattttcc tcctgttgtt tcttcagctg gtgttcctat
    41281 tagtacaatt tttcctttaa aattttcttt taacttactt aatactatac aagcgcctag
    41341 tgtagatgct gcaatccagt tatgtccaca agcatgagca ggttctttat tttctccata
    41401 tccaggaagt gcatcatact cagctaaaaa tgctatagta ggtccatcat tatctcctaa
    41461 ttcagctcta aaagcagtct ccatattaca atatggataa gttgtatcaa atccatattc
    41521 cttcattttc tctactaagt attttgatga tatgtattct tcttccccta attctggatt
    41581 attaaatata aaattacaaa cttcttcaat ttcttttcca agttcattct ctatctcata
    41641 agctctcttc tttatactct ctatcataat ttacctccta ctagaatcct agatataatc
    41701 ctattgtcaa aaataccatt acaagaattg ttaatactac cattaaagga actacgaatc
    41761 taacccactt tgaccaagga atattggcta ctgctagtgc tcccattaaa acacctgatg
    41821 taggtgttat taagttagtt acacctatac caaattgata agctgtaact atcatttgtc
    41881 tggatacatc tactaagtct ccaagtggtg caagtacagg gattgtaagt gatgcaagcc
    41941 ctgatgctga tggtattaaa aatgctatta atccttctat taagaaagtt aaatttataa
    42001 atatagtttt tggtaatcca tttaataaat ttgctgccga atttaaaatt gtatctatta
    42061 taaatccatt ttctgctatt attacgattc cacgtgcaaa tgctatacac aatcctgctg
    42121 taattaaatc tcctgctcca cttataaatg aattaactat ctcatcttgt ttaagccctg
    42181 atgctattcc tgatattata cctatagctg taaatatcat tgcaatttca gttatgtacc
    42241 attctagtct taaaactcca tatatcataa tcgccatacc tattgtgaaa attgctatta
    42301 ccatggcttc tctttttgta aattctttta tgtttgaatc actattaacc attgattctt
    42361 gatttagtga tatatcatgt actaaagatt ttttagggtc ttttttaact ttattggcat
    42421 acatcataac aaatcctata gatatagcca tatatattat aaacataaca actctaaatt
    42481 ctattccaga acctggtact atttgtgata atgcttgtgc tattcccaca gagaaaggat
    42541 tagtagtagc tgctccaaat ccagctgttg ctcctataaa tactgtggcc actgctgtca
    42601 atgaatcata tcccatactg gtcataagtg gaataaatat catataaaaa ggtagtgtct
    42661 cttcactcat tccaaatgtc gttccaccta gtccaaatag aatcattgaa atcggtatta
    42721 ttaaaaattg aaacttcttc attttattta cagtatgagc tataacagct tctatagctc
    42781 ctgttttatt aactatccca aaagctcctc caactattaa aacaaaccca actacttcag
    42841 ccgcatctat gaatccgttt ataggtgctg taaatacatc agtaattcct tgtggatttg
    42901 atggtgcttt ttcataagtt ccagctacaa caactgacct tccgtctata tctactcttt
    42961 caaaatttcc acttggcact atccaagtaa gaattgccat taacactatt agcaaaaata
    43021 ttattgtaag tgtctgtggc atagtaaact ttttcttttt taaactgctc atttttaatc
    43081 tccccacttt ttaaattaaa atattttatt attgcatccc tttatcctat tttgtataga
    43141 tgtaaaaaag ctcaacatgg atacatgttg agctaaatgt gctaaataca acatacctgc
    43201 atcttccctt tatcttgttt aagatagctc tccatcaaaa ttcgggataa ttttgatgac
    43261 agtactgtac ctattaaata cagtcccagc actagcttcg gacactagca cttcggcact
    43321 tactcctttt atattgcctc attggtttcc aacctcttca atattaatct tagtaaatgc
    43381 aacctctacc tcaatctgtt cgttagatga ggtaattctt attaattttt attaattcaa
    43441 agtaattgta gcataatatg tcgagattgt aaatattttc agtataataa ttcttacttt
    43501 atgatatcat tcactttttc acaaataatt ttttcttcat ataatttaaa tactgtagta
    43561 tattttcaat acacacaaac ttcacacaaa tttaaaactc ttataatacc tttataataa
    43621 aagataaaag ccatgtaaac ttatcaattt acatagcttt atatacatta atctactcca
    43681 agtacagctt taagctttga tgctattgta ataactcttg gaccaaatat aacttgaact
    43741 gagtttttct ttttaacaac tccacttgca cccaattcat ttacccagaa accatcttca
    43801 tagaatccat caattgaatt tagctctact ctaagtctag ttgcgcagtt tgtaacattt
    43861 tttatattat tacttcctcc taaaccttct attatagaat gtacaagctt cgtatcttta
    43921 tcatctactt cctcttctac tgcattgttg tatgctgttt taccagcatc tacagattgt
    43981 ctttctctat attctttctt actataaagc ttaacttctt ctgaacttct tcctggtgtt
    44041 tttatatcaa acttcttgat tgcccatgta aatattatat aagtaactat aaaaaatatt
    44101 ggtatcaaaa ataataatgg taacgcatcc actttttgtg gttgtaataa attaggaaac
    44161 atatttctaa atgattctcc cattatagaa acattaaaca tttctgtaaa tacatacgtc
    44221 aatcctgcca gtggtacata tgctacaaaa tacaataatg gtgcaacaaa taagaatgta
    44281 tactctattg gttccgttac ccctaccaac atggctgtta ctattgctgg tatcaatatg
    44341 cttattgctt ttttcttatt agcattatca gccaacttat acatagctaa agctgctgct
    44401 ggtaatattg caaaatgtat taataaacga cctgttgtga agttacgagt tatataagat
    44461 agtgtatctg ggtctcccat ttgagctaat cttatattat taacaccctc gtaaattgct
    44521 ccacctactt ccattgttcc acctactctt gtatattcta tagggaatgc aattaagtga
    44581 tgtataccaa atggtaataa tgctttatcc attgctccaa aagtaaatgt tcctaataat
    44641 ccactagaac ctatccaatt accaagttta gataatgcta accctactgg tggccatata
    44701 aagtacattg ctgtacccaa tactattgat gctataaaca tcataatcat tgaaaatctt
    44761 ggacctgaga aaaaagataa tgcactagga agttctatgt caacaaattt gttatgtact
    44821 gcactagcta caaatccagc tattatagct ccaaatatac tacaatcata tgaaaatatt
    44881 cctaagaaag aaccatataa agaacttcga ctataggctt ctgcctctcc taatccagat
    44941 gccataaacg cttctgctgt aactgtatct gaatttatac catttgctgc taataatgta
    45001 cttataactg tgtgcattcc tataaataaa acaattcctc ctaatgcacc ccatcctttt
    45061 tcctttttag ataatccaaa ggctatacct gtaacaaaga atattggtaa gtatctcatt
    45121 accataaatc ctaagtcgtt tattacttta aagaatacat ttataaatga cccttctttt
    45181 attagtccac taagccctaa tgcagaaacg ttttccatat ttgcaaaagc tgaccctaat
    45241 cctataaata ctcctgctac aacaagtaat ataacaggta tcatcattgc tccagaaagt
    45301 acttgtaatt tatccttcat agtattattt cctccatttt tattgaaatg ccaggctata
    45361 taatgcatat atatatactt ctattgcttt gtataatgta tctatactta tattttcatt
    45421 tgcctgatgt gaatttggat tatccccctc tattgtgggt ccaaatgcaa ctccattttt
    45481 taacactctt gcataagatg cagctccttt tgtaaaaagt tctgcttctt ttccaaaacc
    45541 ttgtttatat gcacttgata atttatttat taattcacta tctggtttta catacagtaa
    45601 ttttaaatcc ttatatatat caactattaa attttctttt tttccaattt cattaattct
    45661 attagttata aactcccaac ttataccctt aggatatcta aagtctattt tcatattcaa
    45721 ttcatttttc tctaagaaga tactcattat acatattgta gaaactccca tatctaaatc
    45781 ttctttatat atccctaatt tttttccatg attatcatct gtaaagtatg aatttaaaat
    45841 atcccttatt ataacgcctt tatcgtttaa ctttttaatt ttctctaaat cttttgctaa
    45901 actaaatgca cagttatatg acctgtgtgg atttctcgat aaagcccttt ctcctgtata
    45961 tcttactaat actttacctt cttctagttc ttctatctct gtataataag ctaatgattc
    46021 tactaaatct ttcttatcat tagttttaaa aacagcctca attttgtcac atacaaatcc
    46081 atcttctttg tttgacttta tatcaactaa attgtgattt ctaaatttat ctttatctat
    46141 tttctttctt agattgaagt ataaaatacc tttttctcca tttactattg ggaagtttcc
    46201 atctggagaa aatgcaaatt ttggctgttc attgacacta aagtaatgct ccatacattc
    46261 ccaagtcgtc tcttcagccc cacctactat caatctaatc tttctctttg gtttttcatt
    46321 aagttctctt aagaaaagta atgcatataa aatccctatc aaaggtcctt tgttatcatt
    46381 aactcctcta ccataaagat agttatcttt ttgacatact ttaaaaggtt tactcttcca
    46441 aagttcctta ttatatattg gcaccacatc tatatgatta agcaccccta ctacttcttc
    46501 tccttctcca tattctatat gaattgcata tccatcaaag tcttttacaa caaaatcttt
    46561 gtcttttgct atttgaataa gcttatcgaa ggcatttctt atttctatcc caaatggttg
    46621 atttatatct gttgtacttt catctctaat tgaaggtatt tcaactaaac ttctaatatc
    46681 ctttatgata ttttcttgat tggattttaa ataatcaata attagttctt tatttactat
    46741 catcttaatt atttatcacc cctttatatt aattttacaa agcatttcag ttcatatcta
    46801 cattataaat tttgaatatt attttttcaa tatttcaaat acttctctaa gttaccataa
    46861 tgatacggta aacaatcaac agattatgac aaaatcatac tatgtctttt actaatattt
    46921 taacttatct attacgtctt ttttaatgta attttattcc aaaaaataca ttaaaaaaga
    46981 tgtagcctta gctacatctt cttatatcta ttcaacttcc attattatgc tattaatatc
    47041 tacatttcca ggttctatcc tatcttttac tgttctctta acagaatcag catcatccat
    47101 tataactaca ggagttatta tatttaaatc atttgacatt ataaagtcta aatctacttc
    47161 cattattaaa tctttagctt ttaccttatc tcctgccttt accttaacat caaacggctt
    47221 tccttctagt gacattgtct ctaaacctac atgtatcaat atatttgtat tatcattgct
    47281 cttaatcatt attgcatgct ttgtatcaaa tacattgact atttcaccat caacaggaga
    47341 atataactta ccttcacttg gtattattgc tactccatct cccatcatct tttgggcaaa
    47401 aactgcatct ggaacatctt ctaagttcac taaatttccc ttaaaagggc tatatatttt
    47461 ctcaatctta ttttcttttt tcttaaatat attaaacata acacactctc cacattataa
    47521 aattttatga taatattttt cttaaagctt ttgctacacc atcatttaca ttagtatcag
    47581 ttacaaaatc tgccatagat tttacttcat ctgtagcatt tcccatagca ataccaagac
    47641 ctgcaaattt aatcatggat atatcatttt cattatcacc tatacaaata acttctttgc
    47701 tatctatacc taatatatca caaaattttt taactgcaat accttttgaa gtacctttac
    47761 tcataacttc aaaatttatc tttccagaac ttactacttc taaatcttct tgttttttaa
    47821 actcttcctt tgcttctctt aaagaatcta cgttttctga gaaaagtata cattttaaaa
    47881 tttcattatc agctttttct aataaagttt cacaacttgc attttctatt atttcaattc
    47941 tgtcttcttc tttattctta ctatttatta atctatatgg atgttcttga cctatttcat
    48001 tgttggatat aactatttta cagcctttaa aatgtttatt taaattatat ttttcaccta
    48061 ttgtatagat tttctttaga gcttccttgc tcaaaacctt cttatactcg tatcccagta
    48121 acttaaaata tgtcccatta gttgatataa tatatatatc atcccctaaa acacttgtaa
    48181 aatatttcat agcattataa ggtcttcccg taactaaggc taccttgata cccttttcca
    48241 tggcttcttt tagtgcttta acattttctt ctgatacttc aaatccttta cccatcaaag
    48301 taccatccat atcagtacaa attaatttat atctcatttg tatcacatcc tagtaagatt
    48361 tttctaatat ttcaacaagg tcttccttct ttccaaaagg taaaaatcct aaaactggac
    48421 agcttgttat ttgttctaat tgttcctttg ttacattaaa ctcagacatt tttgtcttaa
    48481 gtcccactct ctttaaaaaa ctttctattt catttcctaa agttttagct gcttcatcat
    48541 cttccacttt ttctaattct tggtttagca ttctcgctac tttggcaaat ttctttatat
    48601 tatccttata gaacttttct gcaaattttg ggaacactat agccaaagcc tcaccatgag
    48661 gaatatgtgt aactcctcct attatttcac ttagtggatg tggtgctgct gctccagaat
    48721 ttgccagtga acttccccct aatgtatctg ctactgctaa ctttgttctg tattctatat
    48781 tatttttatc ttccattact ttaggtagat agttaatgac taattccata gatttttcag
    48841 acattatctc tgtaaagtca ttagcatttg gatttatata actttcaaat gcatgagtaa
    48901 aagcatcaaa tgctgttgat gcagtaattc tatctggcaa agttaacata agctctgggt
    48961 caactataca ttcttttgag aaacaatttt gatgaaatat tgtatttttc tcgcttcctc
    49021 tcgatattac tgctgcttgt gtaacttggc ttcctgttcc agaagtagtt gatacagcta
    49081 ttaatggtaa ttctttttca cttaccttat cgtatatttt aaatggattt gtatatgtac
    49141 taaacatata atcccaatct attttttcta aaccaaaagt caatgctaat acttttgcag
    49201 tatctatact tgaacctcca ccaacagcta aaacaacatc tacagattct tttttagcta
    49261 attcaaaccc ttcatttatt aattctacag ttgggtttgg ctctactcta tcaaagtgta
    49321 caacttctat acctttttct attaaattca atgtaactct tttatataac ttgtctaatg
    49381 gctctacatt tggagtagta actaacaaac atttctttcc atattttcct attattctag
    49441 gtaattcact taaagttcct tgtccaaaat ttattcttga tggttgataa aagttaatca
    49501 tatttttcct cccttaaatt attgccttca tattcaaatt atacatatca taggattttt
    49561 aaggcaatac cttcactgtt aactcttatt tactaaagct tatggcaatt attagacaat
    49621 taatgtttta ttattaccat atcattatgt aaattttatt aagtacccac gttataattt
    49681 tataatcaat cttattgtat aattgactta ataataaaaa tagagtatat acactttttt
    49741 atgacaaata gcttaaagga gaattaaatt atgaaatatt tgatagacag tgctaatctt
    49801 gatgaaattc aaaagtgttt aaagtatgga tttaagggaa taacagctaa tccatctatg
    49861 tatcttaaag agaaggtaaa cttctatgaa tttattgaga catgtactaa tttaaatcca
    49921 gaaatattaa ctgctgaagt catcggaaat aatttatacg aattaattaa ttcatgtgat
    49981 aagatattaa atataaataa aaacactata ataaaaataa atttctctga agttggctta
    50041 gaacttataa acatattgaa tagtaaaggt gtaaaaactg catgtacctt gattttcaat
    50101 gtaaatcaag catcattagc tataaatgca ggtgctgatt acctattccc ttttataggt
    50161 agaaatgatg aaaatgggaa tgatggtata aaaaacctta cagaaatatg taaccttata
    50221 tcaataaatg gatataatac taaagttgtt gcagcttcta ttaaaaatgt attccactta
    50281 actcaagcat ctctttgtgg atgtgattat gcagctgtaa catatgattt atttaaaaaa
    50341 gcttctttta accagcttac agtagatggt gccaatactt ttgaaagaga ttggtccaag
    50401 gttaataaat agtaactaag atttaggagg gtttgcattg aacaaaagat tattagatat
    50461 tgctgaatta ttactcaaca gctcagacta tattactgta gatactattg ccgaagaatt
    50521 aaaagttagt aataaaacta ttcgtaatga ccttgttatt ttagatgaat ggcttttaga
    50581 gtttaattta tccctagata aaaaaactgg ttcaggggtt attattctag gtaatgaaga
    50641 tgtaaaactt aaagttatta gagatatcaa tgataaatca aactgtatat atgcatactc
    50701 tcctgaagat agaaaaaggt atattctaag taaattattt attaaaaatt ctaagtttag
    50761 gattcgtgat atttgcaatg aactacatgt tagcagagct actgtgcata aagacttggt
    50821 agttattcag aattttttac aaaactttaa agtgacttta gttagaaaaa ccaataatgg
    50881 agtatattta gagggaaaag agaaagatat tcgaaaagca atttttgaac ttgctacggt
    50941 taataaaagt tatactgaac ttaaagaaat attattctcg aaatctaatg aatgcaaaga
    51001 taccccatca acaaaaatat ttaaggagct atttaactat gattttgaga aattatctca
    51061 aatcactcta cacaccttac aagtagaaaa aagtaacctt agtgatgagt attatataaa
    51121 cttcttaata catatagcta tatgtattaa aagaatttca attaaaagat atattaactt
    51181 atctgaaggt ttttttagag aattattaga acatgaatac tttgaaaaat caaaaatttt
    51241 atgtgctaac ctaagtgaag cttttgacgt agaatttatt gatgaagagg tctgttatat
    51301 ccttcttcat atagaaggat tacttaaatc aataaagaaa actactactc ttgaacctct
    51361 taaaaatgaa gaaattacag agtataatct atctgaagca atagctgttc attggggaag
    51421 tatactaaat ttaaacttaa aggatgattt aatattaata aagtctcttg ctaatcacct
    51481 taaatcagtt ctacacagaa taaactatgg atttactatt acaaatccca tattaaatga
    51541 tataaagaga acatttcctt atacttataa ggctgctaaa gagtctaata ctgttatcaa
    51601 acagttgatg aattatgaag tcaatgatga tgaaatagga tatcttgctt tatatttgat
    51661 atcagcaata gatagaagta aatctcccct aaatactatt ttaatatgtc actgtagtga
    51721 tggtgtatct aacttattag tccaaaagtt aagttttgaa tttaatcaga ttaatatagt
    51781 taaatctata cctctatctt ctatttcatt tacgaacttt gatgatgtcg acttaatact
    51841 aaccactgct cctgtagact ttgaacataa tgctgaacta ataaatataa atgctctttt
    51901 aagtaagaat gatatttcta gacttagtac aattataaaa aaactatatt ctgaaaaaaa
    51961 taaaatatta tcttataaat aagcaaaaat aggcttgtct caacatagag ataagtctat
    52021 ttttcattgt ccaaatgata aaaatatatt tgattacata gaaaaaagtg cctcatgaac
    52081 taaaaaattc attttgagac accttcattt tttatattta atctttttat ttaaattttc
    52141 atatttaaag taagttttat tattttattg agcgcaagca ctatttacat tattaatttc
    52201 ttcagtattc ttaccaaact ttgtagttac aaatcctatt gtttttatta tgagtaataa
    52261 agatatcaat gaatatatag atatcataaa gttcttactt attattccag acgctactat
    52321 tataaatcca tctagcataa ataatataac tggcaacttt agaaacttaa tcaccttatt
    52381 aataagcatt gcaactaaat cagttccacc agtactacag ccccttatta ttgctatccc
    52441 tatagcagtt cctaccaata acccacctat cactcctgct actaacagat tgtcagttat
    52501 gtgagtttgt ggtattactt ttaagaataa tggacccata agagttatat ataatgtttt
    52561 tattccaaaa cctttcccta aaaataataa tcccattact aaaaggggca tagatattcc
    52621 taacgatata ttctcaacag gcattgataa gaatgtacta agtacgactg ataatcctgt
    52681 tatacctcct ggagctaatg tatgttctgc aaagaaaaaa ttaactgcta atgccattaa
    52741 aaatgctgat attgttatca gtaaataatc ttttatcatt tcttttttca tttaataacc
    52801 tcctatgatt atattgtttc agtataaaaa atcaattaaa ttagtctagc ttcctgtagt
    52861 ttttcataaa attaatgctt ccttaaaaca ttaagaaagc atctctttta tcatagttta
    52921 ttatcttatt ctaaaccaaa ctttgtagct gcttcatcta tagcttttct acaattctta
    52981 acacctgctg ttataccatc tttatccttg aatattgctg aagataatat tacaacgtct
    53041 ggattacatt ctattaatgg ctccatattg tctgctctta gtccaccatc tattgctaat
    53101 tcacacttag gattcttttc gtctatcatt tttctagctc tttttactaa atcaatagca
    53161 ctctttctcc atccccagtt atcttttcca tcagtttcat caacaccatg aacgactatt
    53221 tgcaatctat ctatatcata tatagcttca tcaacaaaac atagtggtgt gtaacatcca
    53281 attgttaatc ctactttcat accaaattct ctacagtaat ttattatgta tgcaagagga
    53341 gcacctaaaa aatgttctgc tggtattatc aacaagttac atcctgctgc tgctattttt
    53401 tctataaata atctatcaca atctcttgtg tatatatggc attctattgg tttatctgtc
    53461 actggtctta ttgcttctat tatttgatga ccacccataa gttgcatatt ttttaagtca
    53521 tgcatatctg ctgcatctga atgtacgtag tctacacctg catccattgc ttctttaact
    53581 acggctgata tatgaccgta atttacatgt gctaatcctg ctgctatttt tatgtgtttc
    53641 atatttgtat acctccatat tttactttaa ttagtattaa atattagttt ttgatatttt
    53701 tttaatattt tttaatctag tattactttt atttagcatt acttttattg ctgtatatat
    53761 ttcttttatg aacttattct gcttttgtta actgtgaact tattttgctt tgtaaattaa
    53821 tattacactg atatattttt tttcacaaca ctttgactac tccttaaaag tacctttata
    53881 ttgtggtaaa ttatagacat taattgtata taaaaagtta gttttttacc acattcttat
    53941 ggcatttatt gtctatacta actcaattga tatagctagt tcatttaata tataatcaga
    54001 ctataaatat aaatttactc gataaaaagt accattcata acaatattaa tgagagggag
    54061 atgtaaaatg agaaactgta ttattgccca atctggaggc cctacagctg ttatcaatgc
    54121 ttctgcagtt ggtatttatg aaaaaaattt agaaactaaa tactttgata aagtatattt
    54181 tggtatcaat gggattgaag gtatattaaa caaaaatata atagattcag actccttaaa
    54241 caaagataat gtattaaatt taaagcaaac accttcgtct ggtcttggtt cttgtagata
    54301 caaattggct gattataaaa taaattctta tgattatgat agagttataa atatactaga
    54361 tgaatatgaa atagatacat tattttacat aggcggaaat gattctatgg acacagtaat
    54421 gaaattttct caatactgca aaaacactac ttgtaaaata aaatttatag ggattcctaa
    54481 aacaatagat aatgatttat taggaactga caactgtcct ggatttggtt ctgctgcaaa
    54541 gcttatttct acactaataa tggaaacata cttagatttt tctatataca aaaataatgg
    54601 gatatttata gtagaaacta tgggtagaga tactggatgg ttagcagctt cttcttcact
    54661 agctagactt aatgatactc cacttgttga tttaatatac cttccagaaa ttgatttcta
    54721 tgaagataag tttatagaag atgttagaaa aatatttaaa gaaaaaaata aggcttatat
    54781 agttgcatca gaaggtataa gggataaata tggcaacttt atatgtgaac aaaaaacttc
    54841 tgctcatgat aaatttggtc attctcaatt aggtggagta agcaactatc taaagaatct
    54901 tatagtatca tcatcaatta caacaagagt aaaaacctta gaacttggtg taatacaaag
    54961 atgtgctatg catttagctt cagatactga tttaaaacaa gcagctgaag ttggtgcata
    55021 tggtatagaa ctatctaaaa aaggatatac aggagttatg gctatcatta atagaacatg
    55081 caataatcct tataagtatg aagttagtca tactgatatt tctaaaatag ctaacaagac
    55141 aaaatatttt cctaaagagt ggataaatga agagtgtaat tatataactg atgaagctct
    55201 aaattatcta tatccactta ttcaaggtaa accagatatc aaatataaaa atgggctacc
    55261 ttatttttca attatttact aggaaattat tataatttga tatacactca attctatcgc
    55321 tcttaaatac ctaagtggct gttaaataaa attataaatt gttatatgac agtcacttaa
    55381 tcgcttaatt atttaactgt ttagtttttt aattttttaa tcatttttaa tactaatacg
    55441 atttgttgta ataatatctc acacccattt tcattttgga tagattataa ttagttagac
    55501 aataaatatt ttgtcatgat attattatat atagtgatac agatagaaat acttttatca
    55561 tggctagaaa aacaaagatt acacaaagga tttaaaaatc aaacaacttt aattaaaata
    55621 aatggttaaa ataacttcta aatcaactac ataaacaata aaagaacata aagtagttaa
    55681 atctacaatt ataaaattag taagactata acaacttaaa taaaattaaa gttaacaaaa
    55741 gactattaaa gttagcaaat attcctctac ctaaataatt gctttttata aagagttatt
    55801 tattttaatt g
//
LOCUS       DAEPXF010000006        54203 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain HB27
            SAMN13376389-rid12479423.denovo.006, whole genome shotgun sequence.
ACCESSION   DAEPXF010000006 DAEPXF010000000
VERSION     DAEPXF010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13376389
            Sequence Read Archive: SRR10518432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 54203)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 54203)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001069624.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001069624.1
            Genome Coverage       :: 315x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/15/2021 01:54:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,165
            CDSs (total)                      :: 4,076
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 89
            rRNAs                             :: 10, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 10 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 19 of 69
            Pseudo Genes (incomplete)         :: 44 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 10 of 69
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..54203
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13376389-rid12479423.denovo.006"
                     /strain="HB27"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="China: Hebei"
                     /collection_date="2017"
     gene            complement(75..2045)
                     /gene="ftsH"
                     /locus_tag="KRL67_000329"
     CDS             complement(75..2045)
                     /gene="ftsH"
                     /locus_tag="KRL67_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HBH1521236.1"
                     /translation="MNKLLKGAGFYLLVFIIIVGIVQFSGKPTEKIKDLKFSEVYREL
                     TDENISRLYFVNQTSVEGTIKDTNTKFKSYVPTEVMGNKLADEVLDQAKAGKLTFGGE
                     AKPSTPWFVEMLPTLLLIFFMVIIWFVFMNQSQGGGGKVMSFGKSKAKVHKDDEKTRV
                     TFKDVAGLDEEKEDLQEVVDFLKNPKKYIELGARIPKGMLMVGPPGTGKTYLSRAVAG
                     EAGVPFFSISGSDFVEMFVGVGASRVRDLFEQAKKSAPAIIFIDEIDAVGRKRGAGLG
                     GGHDEREQTLNQLLVEMDGFGVNQGIIIMAATNRPDILDPALLRPGRFDRQVVVGTPD
                     VKGREAIFKVHSRNKPLSDDVKMDVLARRTPGFTPADIENLMNEAAILTARKREKKIK
                     METIEEAITKVIAGVAKKSKVISEKERRLTAYHEGGHAVCAHVLEEVSPVHQVTIVPR
                     GRAGGFTMQLPVEDKFYATKNEMKENIVVLLGGRVAEELVLKDVSTGASNDLERVTAT
                     ARSMVTKYGMSSKLGPMSFDSDDEVFLGNSFSSKRNYSEEVAFEIDQETKRIVDGAYD
                     KTRSILQENMDRLEYVAQALLIYETLDAEQFVKAFNKELPLNEIENAVTEENSSKEIE
                     EQLTIKLEKDEEERNNVIDINKNLEDKSDKDK"
     gene            complement(2079..3467)
                     /gene="tilS"
                     /locus_tag="KRL67_000330"
     CDS             complement(2079..3467)
                     /gene="tilS"
                     /locus_tag="KRL67_000330"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HBH1521237.1"
                     /translation="MIFDKVLSTINKHNLIQKGDKIVLGLSGGPDSVCLLHVLNRLKK
                     DFNIEIYAAHLNHQIRGIEAQKDALYVSKLCEDMGIVFFVKSINVPKYCENEGLSLEE
                     GARKLRYEMFYEIKDKIKANKIAIGHNLNDQAETVMMRIMRGTGLKGLKGIDYIRDNC
                     IIRPILDVERNEIEEYCEAYNLNPRIDKTNLENIYTRNKIRLDLLPYMKDNFNSNVIE
                     SIVRMSNSLKSDNDYIEKEAEAKFREVSNIKEKGFVEINLDDFVCLHDAIKVRVLRNS
                     IKHILGDTNFVDQRHIEDIMSLEDNSKVNKMLTLPRNIFVYRKKDSIILTNEEIVNEE
                     IEFYYNVPSNGFIKIKELKQIIETQVMSIDRYKSMKLDNSSKGFDFNKVKGGIVIRSR
                     RQGDKIKLAMGSKKVKDLFIDLKIPREERCKIPIITDSEGIICVGDYKISENYKIDEN
                     TKEVLKINFNKL"
     gene            complement(3650..4078)
                     /locus_tag="KRL67_000331"
     CDS             complement(3650..4078)
                     /locus_tag="KRL67_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1934 domain-containing protein"
                     /protein_id="HBH1521238.1"
                     /translation="MEEKSLSVKISINTRQYDEKGNMDTIEMTAFGKIFYKNDGIYVI
                     YKEKEENIEITNTIKILKNEVSIKKFGAINSTMLFKCGESSTTKYVTPQGTLLIDIDT
                     RELDINIQEGEHIKLKIDYNIKVQDLFLGRNKIDIYIGTK"
     gene            complement(4095..4901)
                     /locus_tag="KRL67_000332"
     CDS             complement(4095..4901)
                     /locus_tag="KRL67_000332"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HBH1521239.1"
                     /translation="MNNKPIGVFDSGLGGLTVLKEIMKILPNEDIIYFGDTARIPYGS
                     RSKETIIKYTFQAINFLKTKGVKAIVIACNTATARSLKEAQEKYDIPIIGVIEAGART
                     AVSSTKNKIVGIIGTEGTISSKAYNLEISKIDESIEIVNKACPLFVPIVEEGWANTEV
                     AKLTAKIYLQELKEKNIDSLVLGCTHYPILKRTIGEEVGEHIKLVNPAKETAKDLKKI
                     LEVQNIINNTEIHGTYQYYVSDIPEKFSDIAREFLKKKIDKIQKVEIQKY"
     gene            complement(5103..5588)
                     /locus_tag="KRL67_000333"
     CDS             complement(5103..5588)
                     /locus_tag="KRL67_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall hydrolase"
                     /protein_id="HBH1521240.1"
                     /translation="MATLLALTFIFMPIKEVFAYEPTEKIKKVNKQEKNIAKKEVINL
                     SDNDLMLLSKLVAGEARGESYEGQVAVAAVVINRVLDPRFPDTIEEVIYQKNAFSVVL
                     DGSIKMAPTDSARKAAQEALYGTDPTNKAVYFWNPEIATCKWINRLNPYMRIGNHVFA
                     K"
     gene            complement(5639..6298)
                     /gene="spoIIR"
                     /locus_tag="KRL67_000334"
     CDS             complement(5639..6298)
                     /gene="spoIIR"
                     /locus_tag="KRL67_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein R"
                     /protein_id="HBH1521241.1"
                     /translation="MRKIKIRLGLLILSLISVISIMTIVINGEVKKVDNISKDYKDKL
                     IRFHVIANSNTDEDQELKLKVRDEVIKYLQPKLQNSKSIKESEAIIKKEYSSLEEISK
                     NIILKNGYNYSVKVGIQYSNFPTKQYSNIVLPAGEYKALKIIIGKGEGKNWWCVMFPP
                     LCFVDESNGVIDKSTDDKLKEVLTDKEYKLIKQDTPKKTSRVKIKFKVLEVVKDLEKK
                     F"
     gene            complement(6443..7123)
                     /locus_tag="KRL67_000335"
     CDS             complement(6443..7123)
                     /locus_tag="KRL67_000335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBH1521242.1"
                     /translation="MENLTKLNLDNYKPLRDVVFENLREAILEGKLKPGQRLMEVQLA
                     EQLGVSRTPVREAIRKLELEGLVVMLPRKGAYVANMSLKDIMDVLEVRASLEGLAAYL
                     AAERISDEDIKKLKDISEEFKKSTLESDVDALLKLDVEFHECIFKATNNKKLIQLINS
                     LWEQVYRFRVTYMSAYDTSHNIVEEHKYLLDAIIKGDSELSKKYATEHIEKAEQFMID
                     KAMISKDL"
     gene            complement(7107..7997)
                     /locus_tag="KRL67_000336"
     CDS             complement(7107..7997)
                     /locus_tag="KRL67_000336"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HBH1521243.1"
                     /translation="MDFIRLKSRAKINLSIDVLGKRQDGYHFVEMIMQTIDLYDIVKI
                     KELDEDEIKVKSTSLDIPLDEDNIVYKAAKILKNKFYIKKGVEIFIEKNIPVAAGMAG
                     GSSNAAAVLVGLNHLWKLRLSEDELKEIGLNLGADVPFCISGRPALAQGIGEKLTNIK
                     GLPCDTNILICKPDLFVSTKEVYQGLDLNNIKKRPNNKYLIECLKSEDIKAVSESMVN
                     ILENVTISKHKEISDIKQVMMKNNALGSMMSGSGPTVFGLFKNKEDALIGKKELLKKY
                     KQVYVVNSSQKGVEICGEFN"
     gene            complement(8115..9665)
                     /locus_tag="KRL67_000337"
     CDS             complement(8115..9665)
                     /locus_tag="KRL67_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3794 domain-containing protein"
                     /protein_id="HBH1521244.1"
                     /translation="MELIKDVIKVDNRIDFGKFQTFIEAEAVVPDKKLDVYEIVKTEG
                     YISLKKIEMADGKILCRGSFNYNVIYIADDKNTISNVDGKVDINEVIEKDNVVQDMEY
                     MLFSEVEHMDCTIMNERKIKVGALMNIRGSLFEKQRLDIVKDVAQVEGIQKHRKEICF
                     QDIVGIEKAESSIRDTITINTEEIQSIISLNPCVKVKESRVTDNKVIIGGVLEINPLA
                     CTYEGELVELDRVGIEFTQFVEVPGVSDGMTEEVLLSMSDFNHIFKQNSESNTGLLEI
                     DCMACCKVKVTDEVTREVLQDAYSPQKIIKFDHKPIQLNKTLRRSEETFMVREGIRND
                     NDDIQIKDIVSVCPTMSIENSYIEGNKSIIQGIIKVEILFVPVEGLKLVYKISEEIPF
                     EHDIEMDNLTDTASVFNTACIDKVEVDLNREQIDLIIKIKRFTEALDKKAENFIIKGE
                     DQGVYDLSKAPSIIVYICKEGDTFWNIAKKYNTTENEIAELNDIKLDEPIKPGKCLIL
                     EKKVVLVD"
     gene            complement(9759..10025)
                     /locus_tag="KRL67_000338"
     CDS             complement(9759..10025)
                     /locus_tag="KRL67_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Veg family protein"
                     /protein_id="HBH1521245.1"
                     /translation="MATVQTLDKIRVSLERHIGKKILLKANKGRKQIITKEGILEKVY
                     PSVFVIKLDDESNGYPRVSYSYSDLLTSNVKLQVFRDQDKLQIS"
     gene            complement(10173..11033)
                     /gene="yabG"
                     /locus_tag="KRL67_000339"
     CDS             complement(10173..11033)
                     /gene="yabG"
                     /locus_tag="KRL67_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation peptidase YabG"
                     /protein_id="HBH1521246.1"
                     /translation="MKVGDIVARKSHNKDIVFKIVSFGVDENNEKIAILKGIAFRVIA
                     DAYIDDLELVKAPDIKDILIDKNVENLLYKSVRKAKERQKRMTRAVPKLQLNPNTYGM
                     PGKVLQIDGDKEYLKICLDVYAQLGIPAVGVAVSEQNQYKEVRALLEKYNPDILVITG
                     HDAMTIKRGDIEDMSNYRNSSNFVKTVKEARKWQPNLDNLVIFAGACQSNYEKIISAG
                     ANYASSPGRIMIHALDPVFIVEKIACSRIDVVVPIDEVIEQTITGVKGIGGSETRGKF
                     RWAMPKTILH"
     gene            complement(11117..12055)
                     /locus_tag="KRL67_000340"
     CDS             complement(11117..12055)
                     /locus_tag="KRL67_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase"
                     /protein_id="HBH1521247.1"
                     /translation="MKFIDLHCDTIAKLMENVETSELKSNKYSVDIDRLKKGDSLAQT
                     FALFVDTEEVKHPFDYCMSMANKFHEEMKKNSDEIALATNYEEIMKNQSEGKLTALLS
                     IEEGAVLEGKLENLKKFYDLGVRMMTISWNHVNELSFPHNKVEYREKGLTDFGREVVH
                     KMNKLGMLVDVSHISDGGFYEIAKISSKPIIATHSNSRAMMNHSRNLTDDMIKVLANK
                     GGVTGINFFHLFLSDKSESKLEDMVRHIKHIVNVGGIDVVSLGSDFDGIDSKVEIEDI
                     SQMGKLYEPLKKEGFSEDDIEKIYYKNALRVIKEVL"
     gene            complement(12165..12326)
                     /locus_tag="KRL67_000341"
     CDS             complement(12165..12326)
                     /locus_tag="KRL67_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH1521248.1"
                     /translation="MFILRFADDDDNLSVKDFTSLTDLKKYISENNIDKTWHQIEEVK
                     KVIPNLKEE"
     gene            complement(12336..12833)
                     /locus_tag="KRL67_000342"
     CDS             complement(12336..12833)
                     /locus_tag="KRL67_000342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SEC-C domain-containing protein"
                     /protein_id="HBH1521249.1"
                     /translation="MSLYTEWRNLSDNHESQEAEVKFWEEYLKVEAGIYNEILNNKMD
                     VVEGKVSELAEKFGTTNEFFMGFIDGISESLKEDIVLEDVEADKEVSIKIDWEKLYNN
                     MVTVEAHWLYNLQGWEDILPADQRKEIIKAYKKSKTIVKEAKIGRNDSCPCGSGKKYK
                     KCCGK"
     gene            complement(12948..13658)
                     /locus_tag="KRL67_000343"
     CDS             complement(12948..13658)
                     /locus_tag="KRL67_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0489 family protein"
                     /protein_id="HBH1521250.1"
                     /translation="MKNYEYKGFYIEKPVGNNVFSYDKRENKSIYVPKLINGTLNNVR
                     LGNEVVFNEVDENKEIKAKGLENMVEYVLGNKKIYVFDNHNHAFYFWAKSLLKGEFTK
                     GCKLVHVDQHKDTREPENYDVDVNNLKDVFRYTNEVLNVGSFIKPALKYNIFSELIII
                     DSLYGFDLEVESEFVLDIDLDIFSSDMDYIPFDFKLDKIKNLIKKAKVITIATSPYFI
                     NQEYAIKVLKELFNYDII"
     gene            14592..15287
                     /locus_tag="KRL67_000344"
     CDS             14592..15287
                     /locus_tag="KRL67_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HBH1521251.1"
                     /translation="MLVDLNNSSSVFSDTQNNTSLESIYFNKWKGKSWITLGDSITRA
                     NGYQDKLKNILGFSRIDNMGENGQTMVSQSENKSTYTLGKTIDYKVYDLATIFIGTND
                     FRYNKKLGKIKVSGSIRFDETTFTGSYQLLIEKILSSNPSIDLVLITPPQRIRDGYDI
                     DFTNEVGSKLIDYVNVIKSLGEMYSLPVLDLYSEGGISKKNISTFTRDGLHPNDVGYD
                     RISDKMYRFLLSI"
     gene            complement(15301..16497)
                     /locus_tag="KRL67_000345"
     CDS             complement(15301..16497)
                     /locus_tag="KRL67_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH1521252.1"
                     /translation="MNNKKSKWISIPFVVIIIGVFLIHIVSKDKEISASENRTLAQKP
                     TIQDFKFEKYTSEFESYFSDQFPFREELSEIYSKVQIVLGKNKIKNYYLLDDNWIMPT
                     PSKSMSEKELKDAANEINELSKIASDSNKKVYYVSTPNKESMLAHLYPKYVDGLKNDI
                     SNKNAFKSYLDLETINVIDVDEHFLKEFNESQREDLYFKTDHHWNGIGAYEGFKCIIE
                     DMNIVNNISWDSYTQTMYDEGYFLGSYNKNLNKLVKEDETIPYVHLKEKPNYEYYKFD
                     GKTETKCKEEDVIATRKNEKEILYGGAYMFGNACSILKIRNEDALSDKKILIIRDSYQ
                     APTSWLFADIFSEVQLVDPRYIDKLDISIDDIIRDSKADLVMFMYNTSDFKMMIDVMK
                     EQREKS"
     gene            complement(16512..17927)
                     /locus_tag="KRL67_000346"
     CDS             complement(16512..17927)
                     /locus_tag="KRL67_000346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBOAT family protein"
                     /protein_id="HBH1521253.1"
                     /translation="MIFSSLTFLFAFLPVVFILYFLVNDKLKNFVLLLTSLFFYAWGE
                     PKYIFLMLGSIVVNYIFGLKVSSDNKKEQKLWLTLSVIFNISLLVIFKYSNFFVDNFN
                     ALFNSHISIPTIALPLGISFFTFQTMSYVIDVYRKDGRVQRNIFDLALYVSLFPQLVA
                     GPIVRYQTVDEQISKRIYSNEKFAVGVNRFICGLAKKVILANQLGMVADGVFSANIAN
                     LSIAESWLGIICYTLQIYFDFSGYSDMAIGLGKIFGFDFLENFNYPYISQSVSEFWRR
                     WHISLSSWFRDYVYIPLGGNRVSPIKQYTNLFVVWSLTGIWHGANWTFLTWGIYYGIL
                     ISIEKVFLGKLLKKVPQIFRHIYLILIVMIGWVFFRADNIVQASEFVQVLFGVGSSPI
                     CNNSFIMYINDYGYIIILSIIFAIPIIPKLKSILKSKTKKLVESNFVYMLHSTFLVAL
                     MFIVVVILINSTYNPFLYFRF"
     gene            complement(18575..19828)
                     /locus_tag="KRL67_000347"
     CDS             complement(18575..19828)
                     /locus_tag="KRL67_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBH1521254.1"
                     /translation="MESKNNVKRFLIIFILAFGTTAMYSLPYMKSSFYDPMQQALALS
                     HTQIGNLLSLYGLVGMVSYFIGGWFADRFSVRKLITFSLIASGILGFYFSTFPSYSMI
                     LLIFVLWGFTTILTFFSASVKVVRMQGSESEQGRIFGFYEGLSGVSGTLISFIGLYFF
                     GKFAEITVGFKYVVWLYSAASIICGILLFFLVEEKKDSGSSDEGLSIKSLLKVVTMPK
                     AWLIGLIIFSTYLVFSSLTYLSPYLSEVYVMPMTLVSALSIIRTYVIKMGASPVAGVI
                     TDKVGSSIRVMFVGFILMTVSTAAYLVIPESTGFIWIAVINMIILSVILFGFRGIYFA
                     SVSESNISLETTGAVVGFASFIGFSPDAFYYTIAGNWLDKYGQTGYTYIFILSVVCAV
                     IGIFATYALNKINKRENKNLNNKIA"
     gene            complement(19879..21033)
                     /locus_tag="KRL67_000348"
     CDS             complement(19879..21033)
                     /locus_tag="KRL67_000348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HBH1521255.1"
                     /translation="MSNNINQDLETKIIHWGHSADPTTGALATPICQTATFAAKTVEH
                     FEELCMTWGYVYTRECNPTLTELEAKLAMLENAESAISSTSGMGAITSTILALVKSGD
                     HIVSSDGIFSHTKLFMSELLSKFGVEVTFVDAVNPQNVKDAMKPNTKIVYIESPLNPS
                     LDLVDIKTIAEIAHENKSLAIVDSTFGTPIVQRPIDLGADLVIHSLTKFINGHGDTLG
                     GAVAGSKELIDLVRWPSLCCFTGASLPPMSAWMILRGMKTLDMRMKKHCENGLAVAEF
                     LEEEENVELVKYPALKSHPQYELCKTQMNGLGGGVVSFKLKDGINGLTRDQASRRLMN
                     SLELATIATSLGEEHTLVQMNGENLIRIAVGLESSNDIINDFKQAIKKLK"
     gene            complement(21593..22162)
                     /locus_tag="KRL67_000349"
     CDS             complement(21593..22162)
                     /locus_tag="KRL67_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HBH1521256.1"
                     /translation="MIIIYGYCRVSTKNQTDNNSLYQQQQEILNKYNDAKIIKETYTG
                     TTTNRPKFEKIISSLKKGDLLVATKLDRLARSTSEGIQLIEGLFAKEVSVHILNIGLL
                     ENTSMGKFFLTTLLAVAELERNMIIERTQLGKEFAKKKKDFKDGRPKKYSNQQIQYAL
                     GLLNSKPYREVVKMTGISKSTLIRAKKSI"
     gene            22365..22592
                     /locus_tag="KRL67_000350"
     CDS             22365..22592
                     /locus_tag="KRL67_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HBH1521257.1"
                     /translation="MNSLLKEAIDELKNIHCGEVFLLKELFKGYEWNRLEMNIRLKLG
                     KLFLYEVVTNPNLNVEVINKNSAYQQMYKKK"
     gene            complement(22811..23554)
                     /locus_tag="KRL67_000351"
     CDS             complement(22811..23554)
                     /locus_tag="KRL67_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3298 domain-containing protein"
                     /protein_id="HBH1521258.1"
                     /translation="MENYQSTNTCTYNILYVQFNEDFERSKYFSYDVKYPVIYNIRNQ
                     STYVNPVILNNINNAVRMTVNNFKNGLQEEEREYNNAASQNSLPKQSYWVSTSYAVTF
                     NKNYVLSMILNLMGFANNYGPKYNTLNNYNIDLTTGKELLLNDIFRDGVDYINIITDY
                     IKSQINENREMYYSNVELVIPEDQAFYLTDNGIIIYFGVDEIAPESFGIPKFELKFSD
                     FGNFINLRFYCISPDIYVQSRGRAKHFMG"
     gene            23722..24594
                     /locus_tag="KRL67_000352"
     CDS             23722..24594
                     /locus_tag="KRL67_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HBH1521259.1"
                     /translation="MLLLADKWKDYELIDMGNGEKLERWGNYVLRRPDPQVVWPMESE
                     WALWKNPHGHYHRSNKGGGQWEHKKRYPEQWTINYKNLKFHIKPTGFKHTGLFPEQAA
                     NWDWMIEKIKKANRPIKVLNLFAYTGGATVACASAGAEVCHVDAAKGMVNWAKENVEL
                     SGLKNQTVRFIVDDVVKFVEREIRRGKKYDAIIMDPPSYGRGPKGEVWQIEEKLYSFV
                     DLCMGVLSDNPLFFLINSYTTGFSPIVLENVLSTTIGKKTNTGDIYGGELGIPASRDG
                     KILPCGIFGRWEKK"
     gene            24594..25259
                     /locus_tag="KRL67_000353"
     CDS             24594..25259
                     /locus_tag="KRL67_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HBH1521260.1"
                     /translation="MIKVIYEDNHLLVVEKPVNILSQGDNTNDKDMVNLLKDYIKKKY
                     NKPGNVYIGLIHRLDRPVGGVMVFAKTSKAASRLSEQVRSKTFKKTYLAVVHGKIKSN
                     SDILKDFLYKNKKTNMVTVVKKNHKDAKEAELNYNVLDFKENLSLVQVNLKTGRSHQI
                     RVQFASRKHPLYGDQRYGKEINKIGQQISLWSNKIEICHPTTKEKMEFTCHPPDKYPW
                     NIF"
     gene            25280..26137
                     /locus_tag="KRL67_000354"
     CDS             25280..26137
                     /locus_tag="KRL67_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HBH1521261.1"
                     /translation="MSKKFSIGEMSKLHKISIQTLRYYDQIGLFKPVHVDQESNYRYY
                     SIDQFSQLDIIKYLKYLGMPLKEIKSKLDENNEEILKLLTNKINIVDEKIKELELIKK
                     VLSTKKETMEKGMKVDEVGKIVRKHIPQRSILSIEYEEGLSSEETMELSRRQIANILE
                     DNVSVFYGGVSGLVSIDKFLKEDKVTYDNSFVTVERDLFDIKSQKHITEISSGDFICM
                     TYKGPYKENYTAFKKLVDYVEYKRIPIENYIYEIPIIDPLSTNNEEELLTEIQIQVKK
                     REIDLRCLV"
     gene            complement(26293..28839)
                     /locus_tag="KRL67_000355"
     CDS             complement(26293..28839)
                     /locus_tag="KRL67_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HBH1521262.1"
                     /translation="MNIFNKVALQGLKKNRTRTLVTIIGVVLSAAMITAVTTFGVSLM
                     NYMTNGAIIKYGDWHVQFLDVDSSFVQEQKVNDEVANTATFENIGYATLEGNRNPDKP
                     YLFIAGFSKEAFDTLPITLISGRLPENSREILVSSKVATDGGVKFAVGDTISLDVGKR
                     MSENEKLGQSAPYTAGSEVFVPQDKKSYTVVGICRKPVFEESSAPGYTLITKEDKQVK
                     TDSLSLFVTLKNPHQIRSYTSSTGDHAYIFNDNVLRFIGVSDNNLFNMFLYSVGGIVL
                     AIIMLGSIFLIYNSFNMSLNERTHQFGILSSVGATAKQLRNSVLFEGICIGAIGIPIG
                     VAIGIVSIGLVISVIAKNFANILYVNVPLTLTLSIPAIVVAAVVSMVTILISAYIPAR
                     KAANTPVMECIRQTNEVKIESKSVKTSKLTQRVYGLEGVLAMKNFKRNKKRYRSIVLS
                     LVLSIVLFISTSALVADLKQMSNQRSEVTDQDIIFSTQGMKDNEMLKLYDKLKTASGV
                     YEGLYQKLMTYSCIAKVSELTDDYLNYEGSHSMSEMLELPVDMQFLDDRTYLNIIKSL
                     GLPTEEYTGQNAKLIAVSMVTKDNNIIDIFTSSSMNVSLTPETNGKLDTPERQNVNIS
                     FVKVVPPDSPPGIRSSDMKKASFRVLAPYSAKDKFEIPGVSTGIESLSFQSKNPTQSV
                     AEMEQIIESEGVTNEYNLYNMYGMMDENRNYIFIANVFAYTFIIMISLISIANVFNTI
                     STNIKLRRRELAMLRSVGMSEHDFQKMMNFECAFYGMRALFFGLPIAVISSWLIHKVM
                     VTDDIDFVIPWFSIGISIFSVLFIVFITMLYTINKIKKENIIDALRDDMA"
     gene            complement(28839..29525)
                     /locus_tag="KRL67_000356"
     CDS             complement(28839..29525)
                     /locus_tag="KRL67_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBH1521263.1"
                     /translation="MAFLKIEDLCKVYGKNENKVTALDHVSLTIEKGEFTAIIGSSGS
                     GKSTLLHSIAGVDVPTSGKIYLEGQDVYGQSNEKLAIFRRRQVGLIYQFHNLIPTLNV
                     VENITLPILMDKRKVNQERLNDLLELLGLKERKTHLPNQLSGGQQQRVAIGRALMNAP
                     AVMLADEPTGSLDSKNGQEIIQLLKESHSKYHQTLIIVTHDENIALQADRIICISDGK
                     VVRDERKVNR"
     gene            complement(29608..30597)
                     /locus_tag="KRL67_000357"
     CDS             complement(29608..30597)
                     /locus_tag="KRL67_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBH1521264.1"
                     /translation="MFRNREFRKFAILFLLITILTVALGFAISTMTGILSIVSATTFG
                     TAFFVFTKNRYKSIAQISEQINLVLHNANHLYITESDEGELSILQSEITKMTLRIREQ
                     NYALKKEKEHLADSLADIAHQLRTPLTSVTLILSLLENTSDEDERKELIRETEELLIR
                     MDWLITSLLKLSRLDAGIVVFQKEQIDVNNLISSALHQLLIPMELHNITLHIDIPKGV
                     KILGDLNWLSEAIQNIFKNCMESVGDNGKIHIVCEDNFLFTQLTIHDNGAGFKKEDLP
                     CLFNRFYRGKNSSTAGYGIGLALCKTIIMRQGGTITAQNHPQGGAIFVIRFPK"
     gene            complement(30584..31270)
                     /locus_tag="KRL67_000358"
     CDS             complement(30584..31270)
                     /locus_tag="KRL67_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBH1521265.1"
                     /translation="MKRIFLVEDDKAIAKNLILLLNSEGFIVTHVSTRSEALVEIAKN
                     KFDLALIDISLPDGNGFTICTEIKEEQDIPVIFLTASGDESSVVTGLNMGADDYITKP
                     FRPRELIARIRTALRKSGRFGSTFEICGLYVDTATGVVKKNGNEVFLSALEYRLLLVF
                     ISNPKSIITRVRLLDELWDAAGEFVNDNTLTVYIKRLREKIEDDPSSPQIILTVRGTG
                     YRLGGSYVSK"
     gene            31680..32009
                     /locus_tag="KRL67_000359"
     CDS             31680..32009
                     /locus_tag="KRL67_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HBH1521266.1"
                     /translation="MDLNKEVLKGHIDTLILSILNKGDSYGYEIAKTVRDETTFELKE
                     STLYVSLKRLESKGLIKSYWGSDQGLGSRRKYYNITDEGKENLELKIQEWNFIQSVMN
                     KFLKRGE"
     gene            32014..32463
                     /locus_tag="KRL67_000360"
     CDS             32014..32463
                     /locus_tag="KRL67_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH1521267.1"
                     /translation="MKIIEEYLEALYKNDTSKEIQDLKEELKEHLIDSTNEFIENGYD
                     INEAQNKAIERFDGSNGVSVELRSIFKQKIEPKKVLLRKLSNSRIVFLNLFGWLLAAT
                     AYTINRGFGHIAPPWLIILLIISIVILIILTIIIKRLKKEIKNDYNI"
     gene            32530..32907
                     /locus_tag="KRL67_000361"
     CDS             32530..32907
                     /locus_tag="KRL67_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HBH1521268.1"
                     /translation="MVKKILLEKFLDTSGVSYINEKLNLFKDTKWISFGTYIMTVDSF
                     CYREDSKHYNPIMSTFDYESEIFIVNALYKENIYSIKSKKLYRNVSKGDKIIVTIKYS
                     YDKYENLIHEEIHDYHLYPLSLD"
     gene            complement(33090..36296)
                     /gene="carB"
                     /locus_tag="KRL67_000362"
     CDS             complement(33090..36296)
                     /gene="carB"
                     /locus_tag="KRL67_000362"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011862003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HBH1521269.1"
                     /translation="MPKLDSIKKTLVLGSGPIIIGQAAEFDYSGTQACQALKEEGIEV
                     VLVNSNPATIMTDKEIADKIYIEPLTIEFIEKIIEKERPDSLLAGMGGQTGLNLAVEL
                     HDAGILDKYNVKVIGTSIESIKKGEDRDLFREVMKEINQPVIVSDIVTNLEAGLEFAN
                     KIGYPVVVRPAYTLGGTGGGIADTEEELREILSHGLQLSPVGQVLLEKSIKGWKEIEY
                     EVMRDGNGNCITVCNMENVDPVGVHTGDSIVVAPSQTLSDEEYQLLRKASIDIINAIE
                     VQGGCNVQIALNPHSLEYAIIEINPRVSRSSALASKATGYPIAKVAAKIALGYTLDEI
                     ENAVTKKTYACFEPTLDYVVVKIPKWPFDKFRHANRKLGTKMMATGEIMSIGSNFEAA
                     ILKGIRSLETGKYSLVHAPSEGRTLEELKARVVVPDDERLFDLAEMIRRGYKVEMISE
                     ITGVDKWFINKFKWIVEQEEKLKVLKIEDLDKEYLHELKKKGFSDKGISDLMKISPEK
                     LYELRSLYNIQPVYKMVDTCGGEFEALSPYYYSTYEQYDEVVVSDKRKVIVLGSGPIR
                     IGQGIEFDYCSVHCVKSLRKMGIETIIVNNNPETVSTDFDTSDKLYFEPLTEEEVLNI
                     IEKEKPEGVILQFGGQTAIKLAKFLHEKNIPILGTDFRDIDAAEDREKFDDLLEKLDI
                     NRPKGKGVWTTNEGVEIAKELGYPVLVRPSYVLGGQGMEITYNEEKLIQYLDDAFARD
                     HKNPVLIDKYLTGREIEVDAICDKEDILIPGIMEHLERAGVHSGDSTTMYPSQNISDE
                     IKEKIVEYTKKIALDLNVLGMVNIQFIEFQNELYIIEVNPRASRTVPYISKVSGVPIV
                     DLATKCMLGAKLKDLGYGTGVYKEPKLVSVKVPVFSMSKLSKVEVSLGPEMKSTGEVL
                     GVGENLEEALYKGFLSAGRHMSDERGVVLATVNNHDKDEFIEIAKDMKELGYTFVATE
                     GTAKSLRENGIEAETVNRVEEPRPNILDAIRNKQVDIVINTPTKGNDSTRDGFKMRRT
                     AIEFSTEIMTSLDTLKALVEVKKKHLNKDKLKVYNIAE"
     gene            complement(36476..37525)
                     /gene="carA"
                     /locus_tag="KRL67_000363"
     CDS             complement(36476..37525)
                     /gene="carA"
                     /locus_tag="KRL67_000363"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002845470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HBH1521270.1"
                     /translation="MKARLILEDGTVFIGKAFGYLEESVGEVVFNTSMIGYGEVLTDP
                     SYYGQIVTMTYPLVGNYGINLSSAESEKVQVKGFIVREKSDSPSNFRCEIDIDQYLKQ
                     NKVIGLEGIDTRALTKILRNNGTMKGIITLEDSKLEDVKHKLDKFSNTEAVRTVTRKE
                     VEHIKGNGPKVAVMDFGVKRNILRSFIARGCDITIFPATTSPEDVLSINPDLIFLSNG
                     PGDPEDLEDVIENIKALIGKKPIVGICLGHQLLALALGGKTAKLKFGHRGGNHPVKDL
                     EEGKVFITSQNHGYYVSEVPEQMKVTHINLNDNTVEGMRHEKLDVYSVQYHPEACPGP
                     KDNDYIFDKFLELVK"
     gene            complement(37782..40988)
                     /gene="carB"
                     /locus_tag="KRL67_000364"
     CDS             complement(37782..40988)
                     /gene="carB"
                     /locus_tag="KRL67_000364"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011862003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HBH1521271.1"
                     /translation="MPKLDSIKKTLVLGSGPIIIGQAAEFDYSGTQACQSLKEEGIEV
                     VLINSNPATIMTDKEVADKIYIEPLTIEFIEKIIEKERPDSLLAGMGGQTGLNLAVEL
                     YEKGILDKYGVKIIGTSVESIKKGEDRDIFREVMKEINQPVIVSDIVTDLQAGLDYAL
                     TIGYPVVVRPAYTLGGTGGGIADNEEELREILSHGLQLSPVGQVLIEKSIKGWKEIEY
                     EVIRDSKGNCIVVCNMENIDPVGVHTGDSIVVAPTQTLSNKECAMLKKASLDILNAVE
                     VQGGCNVQFALNPHSFEYAVIEINPRVSRSSALASKATGYPIAKVSSKIALGYTLDEI
                     ENAVTKKTYACFEPTIDYVVAKIPKWPFDKFKKANRKLGTKMMATGEIMSIGSNFEAA
                     ILKGIRSLETGKYSLVHTPSEERSIEELKKRVVVPDDERLFDLAEMIRRGYKVEMIEQ
                     ITGIDKWFINKFKWIVEQEEKLKVMKIEDLTKDYLLELKKKGFSDKGISDLMKISPEK
                     LYELRSLYNIKPAYKMVDTCAGEIDAISPYYYSTYEQYDEVVVSDKKKVIVLGSGPIR
                     IGQGIEFDYCSVHCVKSLRKMDIETIIINNNPETVSTDFDTSDKLYFEPLTEEEVLSI
                     IEKEKPEGVILQFGGQTAIKLAKFLHEKNIPILGTDFDDIDAAEDREKFDDLLERLDI
                     NRPKGKGVWSLNEGIEVANELGYPVLVRPSYVLGGQGMEITYNEEKLSQYLQDAFDRD
                     HKNPVLIDKYLTGREIEVDAICDKEDILIPGIMEHLERAGVHSGDSTTMYPSQNISDE
                     IKEKIVEYTKKIALDLNVIGMVNIQFIEFQNELYIIEVNPRASRTVPYISKVSGVPIV
                     DLATKCMLGAKLKDLGYGTGVYKEPKLVSVKVPVFSMSKLSKVEVSLGPEMKSTGEVL
                     GVGENLEEALYKGFLAAGRHMSDERGVVLATVNNHDKDEFIEIAKDMKELGYTFVATE
                     GTAKSLRENGIEADIVNRVEESRPNILDAIRNKQVDIVINTPTKGNDSTRDGFKIRRT
                     AIEFSTEIMTSLDTLKALVEVKKKHLNKDELKVYNIAE"
     gene            complement(41041..42084)
                     /gene="carA"
                     /locus_tag="KRL67_000365"
     CDS             complement(41041..42084)
                     /gene="carA"
                     /locus_tag="KRL67_000365"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011862004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HBH1521272.1"
                     /translation="MKGKLILEDGTVFEGKAFGYLKETIGEVVFNTSMAGYGELLTDA
                     TYYGQIVTMTYPLIGNYGINLEDVESNKVQVKGLIVREKSDNPNNFRCEMDIDTYLKQ
                     NKVIGLEGIDTRALTKIIRNNGTKRGIITLENVSLQDVQSKLEEHCNKDSVKNVTRKE
                     IENIKGTGAKVVVIDFGVKNSVLESLKACNCDITIVPAMTTVEEILNINPDLILLSNG
                     PGNPEDINDIVENIKKLIGKKPLTGIGLGHQILALALGGKTSKLVFGHRGGNHPVKCL
                     ETGKVFITSQNHGYNVSEMPENTEITHINLNDNTIEGMRHKELPIYSVQYIPEYTTKE
                     DLDCIFNKFLALI"
     gene            complement(42110..42826)
                     /gene="pyrF"
                     /locus_tag="KRL67_000366"
     CDS             complement(42110..42826)
                     /gene="pyrF"
                     /locus_tag="KRL67_000366"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HBH1521273.1"
                     /translation="MINGKEKLIVAIDTDEFDKAKELIDRLEDSVDIFKVGLEQYVAT
                     KGKTIDYLKEKGKKIFLDLKFHDIPNTMKSAVRAAVRDNVWLMTIHVSDMEGMRQCAL
                     IAREEAERLNIEKPLVVGVTVLTSLSNQDLQDIGCNMTTEELAIKRAKLAKESGIDGV
                     VCSAQEVDKIVEACGTDFITVCPGIRPKSAEVGDQKRVVTPSDAIKKGAHYLVVGRPI
                     TKAENPRESAINIVREIENA"
     gene            complement(43202..43834)
                     /locus_tag="KRL67_000367"
     CDS             complement(43202..43834)
                     /locus_tag="KRL67_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1847 domain-containing protein"
                     /protein_id="HBH1521274.1"
                     /translation="MYKCGKCNLRGCSNGNMDKLMKICPTREIELQQKAKQLYEEEEN
                     RKIAYNAGLVEAEGYCQYTRLKEIIEFAKKCGYKKIGIAFCIGLKREMQTVQKIFEHH
                     GFEVFSVICKNGAMPKSTIGLDASQTISKCSDEVMCNPIGQALFLNEAKVDLNVILGL
                     CVGHDTLAMKYMEAPITTLVVKDRVTGHNPVAVIYNAESYFEKKLFDKEE"
     gene            complement(43858..44772)
                     /locus_tag="KRL67_000368"
     CDS             complement(43858..44772)
                     /locus_tag="KRL67_000368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH1521275.1"
                     /translation="MELYEAIFYRKSVRNFSSKKIKKPLMEEIKRSCKNLEYLNEDLN
                     IKAHVVDRGHIIHFLMGKECKVKAPHYIVVTSDKGEDYLQNIGFATEGVVLRLTTLGI
                     ATCWLEGNLKREDILEFVDIGNKNFEEDEEDDLDYLAGIKTEEDDENKIENPYIIIAF
                     GYPAKNEDLFRTRYARPDRKPVKDMINKINRNRKWKDILELTRRAPSVKNSQPWYFHK
                     DERGLHLFEKRPKKHCEDMNKVSLGVALRHFDIACIKNKIDVSYEKLPIRNKIGKSYF
                     ITVVEHVKPEEEIQEENVILEKEESQDE"
     gene            45372..46601
                     /locus_tag="KRL67_000369"
     CDS             45372..46601
                     /locus_tag="KRL67_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HBH1521276.1"
                     /translation="MNLNRNLEKYAELAIKVGVNIQPGQILLIKSPIECADFARNALK
                     EAYKCGAKNVYIEWSDEESTLIKYLYAPDEAFHEFPKWTAEQYVDIAKEGGAFLSVYA
                     QNPDLLKDVDPERVANFQKASGKALKEWRSYTLTDKCKWSIVSVPTKDWAKKVFPNLS
                     EDKAIEKLWDAIFKCSRVDGQDPIKAWEEHNENLKSKMDFLNKNNFKTLKYKSSKTDL
                     TLDLPKGHVWLSGASKDPNGISFNPNIPTEEIFGMPHKFKVNGTVYSTKPLVYGGNII
                     DNFFLTFKDGKIIDFSAEKGLDTLEKLIETDEGSHYLGEVALVPYNSPISNTDIIFYN
                     TLYDENASCHFAIGSAYKTCLEGGNNLKDEELDEHGVNDSLTHVDFMIGSADMDIIGE
                     TYDGKQIQIFKNGNWAF"
     gene            complement(46806..49187)
                     /locus_tag="KRL67_000370"
     CDS             complement(46806..49187)
                     /locus_tag="KRL67_000370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine S-methyltransferase family protein"
                     /protein_id="HBH1521277.1"
                     /translation="MEIRQYLKNNILIFDGAMGTMLQQKGLKLGENPEVFGLQNPDKL
                     IEIHTAYLEAGSNVILTNTFGCNELKLDSKYTVEEVIDNAVLVARKAIENVDNTKPRY
                     VALDIGPIGEMLEPMGTLSFDKAYEIFKRQVLQGVKSGVDVIVIETMMDLYEAKVAVL
                     AAKENSDLPIFCTMTFDEGGRSFTGCMPECMVATIEGLGVDAIGVNCSLGPKQLLPIV
                     EKIASRATVPVMVQANAGLPNIVDGEAIYDVDAKEFFEGVKKFVEVGATIIGGCCGTN
                     PSFIKEISENINSVTKGCIEKIDECVVCSPSKFVEVQSPTVVGERLNPTGRKSLQEAL
                     KNENVDYAINLGLEQVNAGAQILGVNVGLPEIDEKKLMPKLIREIQAVVDTPLQVDSS
                     NVEALEQGLRYYNGRTIVNSVNGKEESLESILPIVKKYGSCVVGLTLDEKGIPKKAEE
                     RFEIAKRIVNRAVSYGIKPKDIFIDCLSLTVSAQQEEAIETIKAITMVKTLGVKTILG
                     VSNISFGLPNRKALNASFLTLALGAGLDLAIINPNEYSMMEAINSFKILNNTDKGCIN
                     YINQYSNINNSKKSDSSTKIDKDLPLDILVERGLKDEAKNITLNLLKEKDENYILDEI
                     LIPALDKVGKRYDSGDIFLPQLIQSAETVKVSLNTIKETLLSKSSNNVSKGKIIVATV
                     KGDIHDIGKNIVKIMLENYGYEVIDLGKDVPIEEVVEVAKKRNIKLVGLSALMTTTVQ
                     SMKDTIQALRDNEINAKVFVGGAVLTEEYAEEMGADYYSKDAKSAVEIAKLNF"
     gene            complement(49159..49884)
                     /locus_tag="KRL67_000371"
     CDS             complement(49159..49884)
                     /locus_tag="KRL67_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Vitamin B12 dependent methionine synthase,
                     activation domain protein"
                     /protein_id="HBH1521278.1"
                     /translation="MENNLIGREDISINQSEVLRYLQYRGQEIDKDLKYTIDECVAIT
                     KNKINPRYLSRIYPIRINKDNIVELEGTNLTLESKDIYELLKECNECVIMAATIGIDI
                     EKEIRRHSYSNLTKGLIIDSCGTTAIEEVCDIVQNNLEKVFLKQDKFITMRYSPGYGD
                     LPIEKNIDILNVLNAQKQIGLTITSNGIMIPRKSVVAIIGISNTRINKSKKEKSCENC
                     KNYRNCTYRKGANSCGNKTISEK"
     gene            complement(49874..50329)
                     /locus_tag="KRL67_000372"
     CDS             complement(49874..50329)
                     /locus_tag="KRL67_000372"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HBH1521279.1"
                     /translation="MEKVESFKLDHTKVKAPFVRKCSVLDGVKGDKVTKFDLRFLQPN
                     VESFGTAAMHGLEHLLATYLRDTLDGVIDLSPMGCRTGFYLILWGDVDAKTVKIGLEE
                     ALKKVLESDKMPAATAVECGNYRDLSLFGAKEYAKDVLDKGFSLNIYGE"
     gene            complement(50611..51792)
                     /locus_tag="KRL67_000373"
     CDS             complement(50611..51792)
                     /locus_tag="KRL67_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HBH1521280.1"
                     /translation="MKNKSLLNHMFRPILDLIISCGLTSMLILTSMYIIKLLYFNSEI
                     SVINQLVYQLRDFRSLIGESLFYPFLFTITSLAIYSMISYKRNKQIVDFIRKTNRKNQ
                     KLEIIKSAVNLMDEGNLEKSIDIEYDLDILKDKEKDDIDELAHKINNIVHQLRNITIE
                     ERQAQQTKTDLITNVSHDLRTPLTSIMGYLGVIEEGKYKDEVQMMYYVDIAYEKSKNL
                     NVLINDLFELTKMQNNTIKLNKIEINLVELLSQVVSQFEIYFKQEFMVSRINFNEEKL
                     IVKADPNKLVRAFENLITNAVKYGKDGHYVDIVTEKKEDMAVVKVINYGEPIPVLDLP
                     NIFDRFYRVEKSRNRNVGGSGLGLAITKNIVNLHNGEITVSSDKYQTVFEVQLPIIQS
                     A"
     gene            complement(51782..52474)
                     /locus_tag="KRL67_000374"
     CDS             complement(51782..52474)
                     /locus_tag="KRL67_000374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011948339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBH1521281.1"
                     /translation="MNNTVLVVDDEKEIRDLIEIYLLNSGYNVIKAEDGKEALDILKN
                     ENIHLMVLDIMMPKMDGIEVCRKVRESLNIPILMLSAKSEDMDKIQGIMTGADDYLTK
                     PFNPLELTVRVKSLIRRAYYFNGSNNEDKDVIEVGVVIIDKEKHSVTVAGNEIILTSR
                     EFDILYLLANNKGRVYSTEEIFEKVWKEKYYQSNNTVMVHMSRIRDKIEKYTDGEKII
                     HTVWGVGYKIEK"
     gene            complement(52608..53324)
                     /locus_tag="KRL67_000375"
     CDS             complement(52608..53324)
                     /locus_tag="KRL67_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HBH1521282.1"
                     /translation="MKKFKLIGAIILVVFISVFISKERLLKKDKDPIVVAQHDSEQDV
                     EANSQNKNHSNSISNILLVNKTNGISKNYTPENITKVNIPFVEEATEEEKQMAGEPAK
                     AVEDLVKQANSEGIQFLGSSAYRSYDTQLDTYTRRVKSQGREKADAYVAKPGYSEHQT
                     GLCIDLTNPERWFVGSTKEAKWLAENAHKFGFIIRYPEGKEDITGTAYEPWHIRYVGK
                     DAAEEIYSKGLTLEEYLQNK"
     gene            53450..54184
                     /locus_tag="KRL67_000376"
     CDS             53450..54184
                     /locus_tag="KRL67_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine biosynthesis protein TtcA"
                     /protein_id="HBH1521283.1"
                     /translation="MSIQKLLSKARKAIQDFDMIQENDKIAVGLSGGKDSLTLLHILK
                     NYQRFSPQNFELIAITLNTGGVDNSPLDKLCKEINVPFYEFQTDIKEIVFDIRQEKNP
                     CALCANLRRGALNDNAKKLGCNKVALGHHKDDAIETFLMSMFYEGRVNCFSPKTYLDR
                     QDLTVIRPMIYIEEYMTKKISKDSNYPIITNPCPANGHTRREYIKNLIANLNKEMPDF
                     KRNVFGALNNSEKLFIWDKEKIKNFK"
BASE COUNT        17327 a         9602 c         6215 g        21059 t
ORIGIN      
        1 tttaatatta tactttcagt tttagtattt aatccacatc agataataat cttaaaatga
       61 ccaaggtctt aaaactattt atctttatca gatttatctt ctaaattttt atttatatca
      121 ataacattgt ttctttcttc ttcatctttt tctaatttta tagttaattg ctcttcaatt
      181 tctttagaag aattttcctc tgtaactgca ttttcaattt cattcagagg taattcttta
      241 ttaaatgctt ttacaaattg ttctgcatct aaagtttcat atataagtaa agcttgtgct
      301 acatactcta atctatccat attttcttgt aatatacttc ttgtcttatc atatgcacca
      361 tcaactatac gctttgtttc ttggtctatc tcaaaagcaa cttcttcaga ataatttctc
      421 ttacttgaaa aactattacc taggaatact tcatcatcac tgtcaaatga cattggacca
      481 agtttagaac tcattccata ctttgttacc atacttcttg cagtagctgt aactctttct
      541 aagtcatttg aagcacctgt agatacatcc ttaagtacta gctcttctgc tactctacca
      601 ccaagtaaaa ctacaatatt ttctttcatt tcattttttg tagcatagaa cttatcctca
      661 acaggaagtt gcattgtaaa ccctccagct cttcctcttg gaactattgt aacttgatga
      721 acaggactta cttcttcaag tacatgggca caaactgcat gccctccttc atggtatgct
      781 gtaagtcttc tttctttctc acttattact ttcgattttt tagccacacc tgctataacc
      841 tttgttatag cttcttctat agtttccatc ttaattttct tttctctttt tctcgctgtt
      901 aatattgcag cctcattcat taagttctct atatcagcag gagtaaatcc tggtgttctt
      961 ctagccaaga catccatttt tacatcatca cttaatggtt tatttcttga gtgaacttta
     1021 aatatagcct ctctaccttt tacatctggt gtaccaacaa caacttgtct gtcaaatctt
     1081 cctggtctaa gtaaagctgg gtcaagtata tctggtctat ttgtagctgc catgattatt
     1141 ataccttgat ttactccaaa tccatccatt tcaactagaa gttgattaag agtttgctct
     1201 ctttcatcat gacctccacc aagacctgca cctctttttc taccaactgc atcaatttca
     1261 tctatgaata tgatagctgg agcacttttc ttagcctgtt caaataaatc tcttactctt
     1321 gaagcaccaa ctccaacaaa catctcaacg aaatcagaac cacttatact gaaaaaggga
     1381 actcctgctt ctcctgcaac tgctcttgat agatatgttt taccagttcc aggaggtcct
     1441 accataagca ttccttttgg tattcttgca cccaattcaa tatatttctt aggattcttt
     1501 aagaaatcta ctacctcctg caaatcttct ttttcctcat ctagtccagc tacatcttta
     1561 aatgtaactc tagttttttc atcatcttta tgaaccttag cttttgattt gccaaaactc
     1621 ataacctttc cgcctccacc ttgagactga ttcataaaca cgaaccaaat aatcaccatg
     1681 aagaatatta acagtaatgt aggcaacatt tcgacaaacc aaggtgtaga tggcttagct
     1741 tcaccaccaa aagttagttt cccagcctta gcttggtcta acacctcatc tgcaagttta
     1801 tttcccatca cttctgttgg aacatatgac ttaaactttg tatttgtatc ttttatagta
     1861 ccttctacag aagtttgatt tacaaagtat aatcttgata tattctcatc tgttaattct
     1921 ctatatactt cagagaattt taaatctttt attttttctg taggcttacc tgagaattga
     1981 actataccta ctataattat aaaaacaagt agataaaaac ctgccccttt tagcaactta
     2041 ttcaatatag aagccctcct ttcatacatt gctttgccct ataatttgtt aaaattaatt
     2101 tttaatactt cttttgtgtt ttcatcaatt ttataatttt cactgatttt ataatctcca
     2161 acacatatta ttccttcact atctgtgatt ataggaattt tacatctttc ttctcttggg
     2221 atttttaaat ctatgaataa atctttcact tttttacttc ccatagcaag ctttatctta
     2281 tcaccttgcc ttctgctcct tattactata ccccctttta ccttattaaa atcaaaccct
     2341 ttagatgaat tgtctaattt catactctta tacctatcta tgctcattac ttgggtctca
     2401 ataatttgtt ttaattcttt tattttaata aacccattac taggtacatt gtaataaaat
     2461 tcaatttcct cattaacaat ttcctcattc gttaatatta tactgtcttt ttttctataa
     2521 acaaatatat ttcttggaag agttaacatt ttatttactt ttgaattatc ttctaaagac
     2581 attatatcct ctatatgtct ttggtcaaca aaattagtat ctcctagtat atgttttatg
     2641 gaatttctaa gaactctaac tttgatagca tcatgtaagc aaacaaaatc gtctaaattt
     2701 atctctacaa agcctttttc ttttatattt gaaacttctc taaatttagc ttctgcctct
     2761 ttctcaatat aatcgttatc actttttaaa ctattgctca tcctcactat agattctatt
     2821 acatttgaat taaaattatc cttcatatat ggtaaaaggt ctaaccttat cttatttcta
     2881 gtgtatatat tttctaaatt tgttttatct atcctcggat ttaaattata agcttcacaa
     2941 tactcctcta tctcatttct ttccacatct aatatgggtc taattatgca attatctcta
     3001 atataatcaa ttccctttaa accttttaaa cctgttcctc tcatgatacg catcataaca
     3061 gtttcggctt ggtcattgag attatgacct attgctattt tattggcttt aatcttatcc
     3121 ttaatttcgt aaaacatttc atatcttagc ttccttgctc cttcttctaa tgataatccc
     3181 tcattttcac aatattttgg tacattaata gactttacaa agaatacaat tcccatatcc
     3241 tcacaaagtt tagatacata taatgcatct ttttgagctt ctatccctct tatttgatgg
     3301 tttaaatgtg ctgcatatat ttctatatta aaatcttttt ttaatctatt tagtacatgc
     3361 aataaacaaa ctgaatcagg tcctccagaa agacctaaaa ctattttatc gcctttttgt
     3421 attaaattat gtttatttat agtacttagt accttatcaa atatcatatt tatccctctt
     3481 acacttagtt ttattcactt ttaagtatac tttctataaa aaaacgctaa acccttgtat
     3541 caattttaag aaattaaagt ttaatataaa tataataata ccaaataaat taaataaaag
     3601 gtagtttttt cactaccttt ataaaatttc tggaaaatag ccatagttat tatttagtac
     3661 ctatgtatat atctatttta ttcctaccta aaaataaatc ttgtaccttt atattatagt
     3721 ctattttcaa ttttatgtgt tccccttctt gtatatttat atccaattct cttgtatcaa
     3781 tatcaattaa taaagtacct tgtggggtca catactttgt agtactactt tctccacatt
     3841 taaacaacat tgttgagtta atagcaccaa actttttgat actaacttca tttttaagta
     3901 tctttatagt attagttatt tctatatttt cttctttttc cttgtaaata acatatattc
     3961 catcattttt ataaaaaatt ttaccaaagg cagtcatctc tatggtgtcc atatttcctt
     4021 tttcatcata ttgtctagta tttatactta tttttacact caacgatttt tcctccatag
     4081 attcttcata taaactagta tttttgtatt tctacttttt gaatcttatc aatttttttc
     4141 tttaagaact ctcttgctat atccgaaaat ttttcaggta tatctgatac ataatattga
     4201 taagttccat gaatttctgt attgtttata atattttgca cctctaaaat cttttttaaa
     4261 tcttttgcag tctctttagc tggattaaca agttttatat gctctccaac ttcttcacct
     4321 attgttctct ttaaaatagg ataatgagta catcctaaca ctaaagaatc tatatttttt
     4381 tctttcaatt cttgtaaata tatctttgct gtcaatttag ccacttctgt atttgcccat
     4441 ccttcttcaa caataggtac aaataaagga catgctttat taacaatttc tatactctca
     4501 tctattttag atatttccaa gttgtatgct tttgaactta tagttccttc agttcctatt
     4561 attcctacaa ttttgttttt tgtagaactt acagctgttc tagctcctgc ttctataact
     4621 ccaattatcg gtatatcata tttttcttga gcttctttta aacttcttgc agtagcagtg
     4681 ttacatgcta taactatagc ttttacacct tttgttttta aaaagtttat agcttgaaaa
     4741 gtatatttaa ttatagtttc tttggatctt gaaccatatg gtattctggc agtgtctcca
     4801 aagtatatta tatcctcatt tggtaatatc ttcattatct cttttagaac tgttaatcca
     4861 cctaatcctg aatcaaaaac tcctattggt ttattgttca tctttataca acacctactt
     4921 tacatttttg tgtttaacaa caacttattt atcgctaaaa tttatattta tattcataat
     4981 taatatatta ttacctaaat gattattata gtttatttta cacaaaaaat aaaggagcta
     5041 tcttaaatta gaaaataatt tctacaaaaa gacaactcct ttaactactt aaaaatttaa
     5101 atttattttg caaacacatg atttcctatt ctcatataag gattcaatct atttatccat
     5161 ttgcaagtgg ctatttctgg attccaaaaa tatactgctt tattagttgg gtcagttcca
     5221 taaagagctt cctgagctgc tttacgtgct gagtctgttg gtgccatctt tattgaacca
     5281 tcaagcacaa ctgagaaagc atttttttga tatatgactt cttctattgt gtctggaaac
     5341 ctagggtcca atactctatt tattactaca gctgctacag caacctgtcc ctcatagctt
     5401 tctcctctag cttctcctgc aaccaactta gataatagca tcaagtcatt atcagataaa
     5461 tttattactt ctttctttgc tatatttttt tcttgtttgt ttactttctt aatcttttct
     5521 gttggttcat atgcaaatac ctctttaata ggcataaata taaaagtcaa agctaatagt
     5581 gtagccatca gtttactcca cctgtccatg attaaaacca ctccttacat tcttattttt
     5641 aaaatttctt ctctaaatcc ttcactactt caagtacttt aaacttaatt tttactctac
     5701 ttgttttttt tggtgtatct tgttttatta acttatattc tttatcagtt aaaacttcct
     5761 tcaacttatc atctgtagat ttatctatta ctccattaga ctcatcaaca aaacatagtg
     5821 gagggaacat aacacaccac cagttttttc cttcaccttt tccaatgata atctttaatg
     5881 ctttatattc accagctgga agaactatat tagaatattg tttagtaggg aaattgctat
     5941 attgtatacc tacctttaca ctgtaattat atccattttt aagaataata tttttactta
     6001 tttcttccag actactatat tcctttttta taatagcttc actttctttt atactttttg
     6061 aattttgaag ctttggctgt aaatatttta ttacttcatc tctaactttt agtttaagtt
     6121 cttggtcttc atctgtattg ctgtttgcta taacatgaaa tcttattaat ttatctttat
     6181 aatcttttga tatattgtct acttttttta cctcaccatt tattactatt gtcattattg
     6241 atatgactga tataagactt aatattaata gtcccaatct aatcttaatt tttctcattg
     6301 aaaattcccc cttaagataa atttatatca tcaattatgt ccaattaaat ttaatttatt
     6361 cataatattt tttaatacta ataaaaaaag ctatcttaaa atgattacaa tcattttaaa
     6421 atagcttttt aaaccttata ttttataagt ctttactaat catagcctta tctatcataa
     6481 actgctctgc tttttctata tgctctgtag catacttctt ggatagctca ctatcacctt
     6541 taattatagc atcaagtaaa tatttatgct cttcaacaat attatgtgat gtatcataag
     6601 cagacatata ggtaactcta aatctatata cttgttccca taatgagttt attagttgga
     6661 ttaatttttt attattagtt gctttaaata tacattcatg aaattctaca tcaagtttta
     6721 ataacgcatc tacatcggat tccaaagtgc tttttttaaa ttcttctgat atatctttaa
     6781 gctttttaat atcttcatca ctaattcttt cagcggcaag atatgctgct aagccttcta
     6841 ggcttgctct tacttctaat acatccataa tatcctttaa tgacatattt gccacatacg
     6901 caccttttct aggcaacata acgactaaac cttctaattc aagctttctt attgcctctc
     6961 tcactggagt cctacttact cctaattgtt ctgctagttg cacttccatt agcctttgac
     7021 caggctttaa ttttccttct aagattgctt ctcttaaatt ctcaaagact acatctctta
     7081 atggcttgta attatccaag ttcagtttag ttaaattctc cacaaatttc aacccctttt
     7141 tgactgctat taacaacata cacctgttta tattttttta ataattcttt ttttccaatt
     7201 agagcatctt ctttattttt aaatagtcca aaaactgtag gaccacttcc actcatcata
     7261 gaacctaaag cattattttt catcattact tgttttatat cacttatttc tttgtgttta
     7321 cttatagtaa cattttcaag aatatttacc atgctttctg atacagcttt tatatcttca
     7381 gattttaaac actctatcaa atatttatta tttggccttt ttttaatatt attcaaatct
     7441 aatccttgat aaacttcttt agtagaaaca aataagtctg gtttgcatat caatatattt
     7501 gtatcacatg gtaaaccttt gatgtttgtt agcttttctc caataccttg agctaaggca
     7561 ggtcttcctg atatacaaaa aggaacatct gcacctaaat taagtcctat ttcttttaat
     7621 tcatcttcag ataatctaag tttccatagg tgatttaaac ctactaatac tgcggctgca
     7681 ttagaacttc ctccagccat ccctgctgct acaggaatat ttttttctat gaatatttct
     7741 acaccttttt ttatataaaa tttattttta agtattttgg cagctttata aacaatatta
     7801 tcttcatcta aaggaatatc taaactagtg ctttttacct ttatttcatc ttcgtctaat
     7861 tcttttatct ttactatatc gtataaatct atcgtttgca ttatcatttc aacaaaatga
     7921 taaccgtctt gcctttttcc taagacatca atggataaat ttatttttgc tctactcttt
     7981 agtcttataa aatccattct atcctccttg tttaaagaat aatgcatcac taacaataat
     8041 attatactac aaaaaataaa ataatagggt attataatac cctattatac ttaattactt
     8101 taaatttttt aatattaatc tactaatacg actttttttt ctaaaataag acacttacca
     8161 ggcttaattg gttcatcaag ttttatatca ttaagttctg caatttcatt ttctgttgta
     8221 ttgtattttt ttgctatatt ccagaaagta tctccttctt tgcatatata tacgattatg
     8281 ctaggagctt tagataaatc atatacacct tggtcttcgc ccttgataat aaaattttct
     8341 gctttcttat ctaatgcttc agtaaatctc ttaattttga taatcaagtc tatctgttcc
     8401 ctattcaaat caacttcgac tttatcaata catgctgtat taaacacaga tgctgtatca
     8461 gttagattat ccatttctat atcatgttca aatggaattt cctcactaat tttataaact
     8521 aattttaaac cttcaactgg gacaaataaa atttcaactt ttatgattcc ttgtataatg
     8581 cttttatttc cttcaatata actattttct atagacatgg ttggacaaac gcttacaata
     8641 tcttttattt gtatatcatc attatcattt cttatgcctt ctctaaccat aaatgtttct
     8701 tcactacgtc ttaaagtctt atttaattga ataggtttgt ggtcaaattt tattattttt
     8761 tgaggcgaat atgcatcttg taatacttct cttgtaactt catctgtaac ttttacctta
     8821 caacaagcca tacaatctat ttctaaaaga cctgtgttac tttcactatt ttgcttgaaa
     8881 atatgattaa aatctgacat agacaacaat acttcttcag tcataccatc actaactcca
     8941 gggacttcta caaattgggt aaactcaata cctactctat ccaactctac aagttcaccc
     9001 tcatatgtac aagctaacgg atttatttct aatactccac caataataac tttattgtca
     9061 gtcactctac tttcttttac tttaacacat ggatttaaac ttattataga ttgaatttcc
     9121 tctgtgttta ttgttatagt atctcttata gaactttctg ctttttctat tccaactata
     9181 tcttgaaaac aaatttcctt tctgtgcttt tgtatgcctt ccacctgtgc cacatctttt
     9241 acaatgtcta acctttgctt ttcaaacaaa cttcccctta tattcataag agctcctact
     9301 ttaatctttc tctcattcat tatagtacaa tccatatgct ctacttctga aaataacatg
     9361 tattccatat cttgaacaac attgtctttt tctattactt cgtttatatc tacttttccg
     9421 tctacattag aaatagtatt tttatcatct gcaatataga ttacattata attaaaactt
     9481 cctctacaaa gtatcttccc atctgccatt tcaatttttt taagtgatat gtatccttct
     9541 gtttttacaa tttcatatac atctaatttt ttgtctggta caactgcctc cgcttcaatg
     9601 aatgtttgaa attttccaaa gtctattcta ttgtcaactt taattacatc tttaattaat
     9661 tccatagtct acctccttaa gtattataaa aatatcacac tatatttata tttaaggagc
     9721 aaaaaaaaaa ttacacctat ttttaaatag gtataatttt aacttatttg taatttatct
     9781 tgatctctaa aaacttgcaa ctttacatta gatgttaata aatctgagta actataagat
     9841 actctaggat atccattact ttcatcatct aactttataa caaacacgct tggataaact
     9901 ttttctagta taccctcttt tgtaataatc tgctttcttc ctttattagc tttcaacaat
     9961 attttctttc ctatatgtct ctctaagctt actcttatct tatctagagt ttgaacagta
    10021 gccacaatat caccttcttt aacgtttata tgtttacata atatcacata taaacatttt
    10081 tgtcaaatgt ttaaactaat tattttatat attttttaca aaaatgtcaa tactattttt
    10141 gaagatatac agtataatta tgttaatttt tgctagtgta atattgtttt tggcattgcc
    10201 catctaaatt ttcctcttgt ttcagaacca ccaattccct taacacctgt aatagtctgc
    10261 tctataacct catcaatagg aactactaca tcaattcttg aacaagcaat tttctccact
    10321 ataaaaacag ggtctaatgc atgtatcatt attcttccag gcgaacttgc atagtttgct
    10381 cctgcactta ttattttttc ataatttgat tggcatgctc ctgcgaatat gacaagatta
    10441 tctaaatttg gctgccattt gcgtgcttct tttactgttt taacaaaatt tgaagagttt
    10501 ctatagttac tcatatcttc tatatctcct ctttttattg tcattgcatc atgacctgtt
    10561 attactagta tatccggatt atatttttct agtagtgctc ttacttcttt atactgattt
    10621 tgctctgaaa cagctactcc tactgcaggt attccaagtt gagcatatac atccaaacaa
    10681 atttttaaat attctttatc tccatctatc tgtagtactt ttccaggcat tccatatgtg
    10741 ttaggattta attgaagttt tggaactgct ctagtcattc ttttttgtct ttctttagct
    10801 tttcttactg acttatataa taaattttct acatttttat ctattaatat atcttttata
    10861 tcaggtgctt ttactaattc taggtcatca atataagcat ctgctattac tctaaatgca
    10921 attcccttta gtatagctat tttctcatta ttttcatcta caccaaaaga gactatttta
    10981 aatactatat ctttgttatg tgactttcta gctacaatgt ctcctacctt catagttaaa
    11041 cctccaataa atttttatct tagtttatac tatgttcgat ataaaacttt gtttcctaat
    11101 gtcaaaaaaa catttcttat aatacttctt ttatcactct taaagcattt ttatagtata
    11161 tcttttctat atcatcttca ctaaaccctt ctttttttag aggttcatac agtttaccca
    11221 tctgagatat atcttcaatc tcaacttttg aatctattcc atcaaaatct gaacctagag
    11281 acacaacatc tattcctcca acatttacaa tatgctttat gtgtctaacc atatcttcaa
    11341 gtttactttc acttttatca cttaaaaata agtggaagaa gtttatacct gtaactccac
    11401 ctttatttgc taatacctta atcatatcat cagttaaatt tctagaatga ttcatcattg
    11461 ctcttgagtt agaatgtgta gcgattattg gtttagaaga aatctttgca atttcataaa
    11521 atccaccatc tgaaatatga gaaacatcaa ccaacattcc caatttattc atcttatgta
    11581 ctacttctct tccaaaatca gtaagccctt tttctctata ttctacttta ttatgaggaa
    11641 aacttaattc attcacatga ttccaactta ttgtcatcat tctaacccct aagtcgtaaa
    11701 atttctttaa attttctaat ttaccttcaa gcactgcacc ttcctcaata gataaaagag
    11761 ctgtaagttt accttctgat tggtttttca taatttcttc atagttagta gctaaagcta
    11821 tttcatcact attttttttc atttcctcat gaaatttatt tgccatactc atgcaataat
    11881 caaaaggatg ttttacttct tcagtatcaa caaaaagagc aaatgtttga gctaatgaat
    11941 cccccttttt caatctgtct atatcaacag aatatttatt agacttaagt tcacttgttt
    12001 ctacattttc cattaatttt gctatagtat cacaatgtaa atcaataaat ttcatattta
    12061 taccccctgt atattttata tttatatcta aaaatagaaa tagttgtcct tatagacaac
    12121 tattgacaac tattttttaa tattcttttt aatgttactt aatcttattc ttcttttaag
    12181 ttaggtatta cttttttaac ttcttcaatt tgatgccatg ttttatctat attattttca
    12241 ctaatatatt tttttaaatc agttaaagat gtaaaatctt taactgataa attgtcatca
    12301 tcatctgcaa atcttaaaat aaacatgaga ctcccttact ttccacaaca tttcttatat
    12361 ttttttccgc ttccacatgg gcatgaatca tttcttccta tttttgcttc tttaactatt
    12421 gttttagact tcttataagc ctttattatt tcttttcttt ggtctgctgg tagtatatct
    12481 tcccaacctt gtaaattata tagccaatga gcctcaacag ttaccatatt gttatataat
    12541 ttttcccaat ctatttttat agaaacttct ttatcagctt ctacatcttc taaaactata
    12601 tcttctttta aactttcact tattccatct atgaatccca tgaaaaattc attagtagtt
    12661 ccaaattttt cagctaattc acttacttta ccttcaacaa catccatttt attatttaat
    12721 atttcattgt atattccagc ttcaactttc aaatattctt cccagaattt tacttctgct
    12781 tcttgacttt catgattatc acttaaattt ctccattcag tatataaact catacttttt
    12841 cctcctaagt atacctatat ttttatagtt tttatagtat aaaatctttt agttagattg
    12901 gaagtttctt ctttttgatt ataccatatt ctatttaaag ttaactctta tattatatca
    12961 taattaaaca attcttttaa tacttttata gcatactctt ggtttataaa ataagggctt
    13021 gtcgcaattg tgattacttt ggctttttta attaaatttt ttatcttgtc taatttgaaa
    13081 tcaaatggta tatagtccat atcacttgaa aatatatcca aatctatatc aaggacaaac
    13141 tctgactcta cttctaaatc aaatccataa agactatcta ttattattaa ttctgaaaat
    13201 atgttatatt ttaaagcagg ctttataaaa cttcccacat ttaaaacttc atttgtatat
    13261 ctaaatacat cttttagatt atttacatct acatcatagt tctcaggttc tctagtatcc
    13321 ttatgttggt ctacatgcac taacttgcat ccttttgtaa actcaccctt taacaaagat
    13381 tttgcccaaa aataaaatgc atggttatga ttgtcaaata catatatctt tttatttccc
    13441 aggacatact caaccatatt ctccaatcct tttgctttta tttccttatt ttcatctact
    13501 tcattaaata ccacttcatt gcctaacctt acattattta atgtaccatt tataagtttt
    13561 ggaacgtata tgctcttatt ttctctctta tcatatgaaa acacattatt tcctactggt
    13621 ttttctatat aaaatccttt atattcataa tttttcataa aagttaaatt cctccattat
    13681 tataatatat aagctaaatt cacctatttt tgtattcttg gtgactatca ctaatatctt
    13741 ctctcaatgt gtattataca ataagtataa gatacattta taaattagta ttatttcttg
    13801 tttaaatata aacgagatta tctcaaaatt gatttttttt aatcaaattt taagttcaac
    13861 tcatttttga atttctgtat ttacattatt gtctgaagat attttttaag ttcattgtat
    13921 aaagataatt tttgtttttg taaaagtgtt atttttattg aaggctcaat gcatcctatt
    13981 atatcttcta tatcatagtt aataaaacta gatatgtctt ctaaattatt acttaaaatt
    14041 ttcagatgat aatgtgctaa aaccttcttc attttgtaat aaatatttgc taaatcaaag
    14101 tactcacttg cagattctat tatatttttt gttgaattta gatacttaat ttattttatt
    14161 aatttgtctt cttctgttga gttgttgcaa ttcttattat agaaagcaat taattcaaaa
    14221 acaaaattta aaattacatc tttacgattt tatatatcct gatttgaatt tgttccaaat
    14281 actttaatat attctaattt ttgaccaata tccttttctc cactttctgt taattgttca
    14341 acacttaaat tatctaattt ttcatactta caaatcaatc aaagcaacat agttatatat
    14401 tttattcaaa ttttttatag gtatatttag tttgagatat ccatgaattt tccactttat
    14461 ttgcatctaa attatgttaa gaataattta tgaatcttaa tacataataa aacatatagg
    14521 agtggtatca tatgaaaaag agcaccatat gttttttatt agttacaatg gttagctgta
    14581 ttacttcata tatgttagtt gatttaaata attcatcatc tgtatttagt gatacacaaa
    14641 acaatacatc cttagaatcg atatatttta acaagtggaa aggcaagtca tggataactc
    14701 taggtgatag tataacaaga gctaatggat atcaggataa acttaagaat atattaggat
    14761 tctcaagaat tgataatatg ggtgaaaatg gtcaaacaat ggtatcccaa tctgaaaata
    14821 aatcaaccta tacacttgga aagacaattg attataaggt atacgatttg gcaacaattt
    14881 ttattggaac taatgatttt aggtataata aaaaacttgg aaaaattaaa gttagtggaa
    14941 gtattaggtt tgatgaaact acctttacag ggtcatatca actgttaata gagaaaatat
    15001 tgtcatcaaa tcctagtata gatttggttt tgattactcc accccaaaga attcgtgatg
    15061 gatatgatat tgactttacc aatgaagttg gaagtaaact aatagattat gtaaatgtta
    15121 taaaatcttt aggtgagatg tattcgcttc ctgtattaga tttatactct gagggtggga
    15181 tatcaaaaaa gaatattagt acatttacaa gagatggtct tcatccaaat gatgttggat
    15241 atgatcgtat atcagataaa atgtatagat ttttattaag catatagcaa agagaaaaga
    15301 ttaactcttt tctctttgtt ctttcattac atctatcatc attttaaaat cgcttgtatt
    15361 atacataaac atgactaaat cagctttact atctcttata atatcatcaa tagatatatc
    15421 aagtttatca atatatctag gatctactag ctgcacctct gagaatatat ccgcaaataa
    15481 ccatgatgta ggagcttgat atgaatctct tataataagt atttttttat cagataatgc
    15541 atcttcattt ctaattttta aaatagaaca agcattgcca aacatatatg caccaccata
    15601 taaaatttct ttttcatttt tacgagtagc tataacatct tcttccttgc atttagtttc
    15661 tgtttttcca tcaaatttat agtattcata attaggcttc tcttttagat gaacgtatgg
    15721 aatagtctca tcttctttaa ctagtttatt taaattttta ttataacttc ctagaaaata
    15781 tccttcatca tacattgttt gagtataact atcccaagaa atattattta ctatattcat
    15841 atcttcaatt atacatttaa agccttcata agctcctatt ccattccagt gatggtctgt
    15901 tttaaaatac aagtcttctc gttgagattc attaaactct tttaaaaaat gttcatctac
    15961 atctataacg tttatagttt ctaaatccaa ataacttttg aaagcatttt tattactaat
    16021 atcatttttt agcccatcaa catatttggg gtataaatgt gctagcatac tttctttatt
    16081 tggagtagaa acataataaa cttttttatt tgaatcagat gctattttag ataattcatt
    16141 tatttcattt gcagcatctt ttaattcttt ttcagacatt gattttgatg gtgttggcat
    16201 aatccagtta tcatctagta aataatagtt tttaatttta tttttaccaa ggactatttg
    16261 taccttagag tatatttcag ataactcttc tctaaatgga aactggtctg aaaaataact
    16321 ttcaaattcg cttgtatatt tttcaaattt aaaatcttgt atagttggtt tttgagccaa
    16381 cgttctattt tcagatgcac taatttcttt atctttactt actatgtgta taaggaatac
    16441 tcctattatt ataactacaa aaggaatcga tatccacttt gatttcttat tattcatttt
    16501 aatttgcccc cttagaatct gaaatataaa aatggattat atgtagaatt tatcaatatt
    16561 acaactacta taaacattag tgccactaaa aaagttgagt ggagcatgta tacaaaatta
    16621 ctttcaacca gttttttagt ttttgatttt aatattgact taagcttagg tattataggt
    16681 attgcaaata taatagataa aattataata tatccataat catttatata cataataaag
    16741 gagttattac atattggact tgagccaact ccaaataaaa cttgtacaaa ttcagatgct
    16801 tgcactatat tatctgctct aaagaatacc catccaatca ttactattag tattaagtat
    16861 atatgtctaa atatctgtgg tacctttttt agtaatttac ctaaaaagac tttttctata
    16921 ctaattaaga ttccataata tataccccat gtaagaaatg tccagttagc accgtgccat
    16981 attcctgtta aagaccatac cacaaataaa tttgtatact gcttaatagg agaaactctg
    17041 tttccaccta aaggtatgta tacatagtct ctaaaccaac tacttagcga tatatgccat
    17101 cttctccaaa attcagatac agattgtgat atgtatggat agttgaagtt ttcaagaaaa
    17161 tcaaatccaa atattttacc aagtcctata gccatatcac tgtaacctga aaagtcaaag
    17221 tatatttgaa gtgtatagca tattattccc agccacgatt cagctattga aagattcgct
    17281 atatttgctg aaaaaactcc atctgctacc attccaagtt gatttgctaa aataactttc
    17341 ttagctagtc cacatataaa tctatttact cctactgcaa atttttcatt agaataaatc
    17401 ctcttactta tttgctcatc tacagtttga tacctaacga taggacctgc tacaagttgc
    17461 ggaaataaac ttacatatag tgctagatca aatatatttc tttgaactct tccgtctttt
    17521 ctgtatacat caattacata gctcattgtt tggaatgtga aaaatgatat acctaagggt
    17581 agtgctattg ttgggatgct tatatgagaa ttaaataaag cgttaaaatt gtctacaaag
    17641 aaattagaat atttaaaaat taccaataat gatatgttaa aaatcacaga taatgttaac
    17701 cataatttct gttctttctt attgtctgaa gatactttaa gtccaaatat ataattgact
    17761 actatagacc caagcattaa gaatatatat ttaggttctc cccatgcata aaagaataga
    17821 ctcgtaagta ataacacaaa atttttaagt ttatcattaa ctaaaaaata gagtataaaa
    17881 actacaggta aaaacgcaaa taaaaatgtt aagctactaa aaatcatatt tttatatcaa
    17941 tttaaatcct ctaaattgat atattcacct cctttaatca acccatttat ggatctattt
    18001 tattgtataa ctacatttga acaaactttg ttttaaaaaa atgtataata gtggactaac
    18061 atagttatat ttagacaaaa tgtattagta tgtaaattta agtaagagta ggacttaatt
    18121 ttataattag aaccatctct tgataatcat aaatgttatt tttgacagac ttatgcccta
    18181 tataaatata aaatgatata aacgcataaa atcacaatat ttttttgaat ctaagtctat
    18241 tctatattac ttatataggt taacataata acataaaaaa catattttgc aaatattttt
    18301 aatttaattg taattatttg ttattgaaaa tctgctttat atatactcat ctcgacacaa
    18361 aatattataa taacaagtag cacctgtaaa tctccaaaca tattgcaaat acttattcac
    18421 atatattttt cagttctaat tataaattca ttatattttt agttgtgtaa ataaattttc
    18481 tataaaaata aatgcattgt attatttttt gcctaaaaag gggatgactc aaaattttat
    18541 tttgagccat ccccatataa ataattataa atatttaagc aatcttgttg ttcaaattct
    18601 tattttctct tttgtttatc ttatttaaag cataagtagc aaatatccct ataactgcac
    18661 atactacaga taaaataaat atatatgtat atcctgtttg tccatattta tctaaccaat
    18721 tacctgcaat agtgtaataa aatgcgtctg gagaaaatcc tataaatgaa gcaaatccaa
    18781 ctactgctcc tgtagtttct aaagatatat tagactctga aacagatgca aagtatatac
    18841 ctctaaatcc aaataaaatt acacttagta ttatcatgtt tattacagct atccatataa
    18901 atcctgtact ctcaggaatc accaagtaag cagctgtact tactgtcatt aggataaatc
    18961 ctacaaacat tactcttata gatgaaccaa ctttatctgt tataacacca gcaactggag
    19021 atgctcccat ttttataaca taagttctta ttattgataa agcagaaact agagtcattg
    19081 gcattacata aacttctgat aagtatggac ttaaataagt taaacttgag aatactaaat
    19141 aagttgaaaa tattataaga ccaattagcc aggcttttgg cattgtcact acttttagta
    19201 atgacttaat acttaatcct tcatctgatg atccactatc ttttttctct tctactaaga
    19261 agaatagaag tataccacat attatagatg ctgcagaata taaccatact acatatttga
    19321 atcctactgt tatttcagca aatttaccaa agaaataaag acctataaaa gaaattaatg
    19381 tacctgatac acctgataac ccttcataaa aaccaaatat tcttccttgc tctgactcac
    19441 ttccttgcat tctaacaacc ttaacagatg ctgagaagaa ggttaatatt gtcgtaaatc
    19501 cccataatac aaatattaaa agtatcatac tatatgacgg gaatgtagag aaataaaatc
    19561 caagtattcc tgaagcaatt aaagaaaaag ttattagttt tctaactgag aatctatctg
    19621 caaaccatcc tcctataaaa taagatacca ttcctactaa tccatataaa cttaataagt
    19681 tccctatttg tgtatgactt aatgctaaag cttgttgcat tgggtcataa aatgatgatt
    19741 tcatatatgg taaactatac atagctgttg ttccaaatgc caatatgaat attatcaaaa
    19801 atcttttaac attatttttg ctttccattg attctctctc cttaaaattt tattgtttat
    19861 aatctcattc atgtaatttt attttaattt ttttattgct tgtttaaagt catttattat
    19921 atcatttgaa gattccaatc ctacagctat tcttattaga ttttctccat tcatttgaac
    19981 taatgtatgc tcttccccta agcttgttgc tattgttgca agctctaaag agttcattaa
    20041 tcttctacta gcttggtctc ttgtcaatcc attgatacca tccttaagtt taaatgaaac
    20101 tacacctcca cctaaaccat tcatttgagt tttacaaagc tcatattgag gatgagattt
    20161 taatgctgga tactttacca attccacatt ctcttcttcc tctaaaaact ctgctactgc
    20221 taatccattt tcacaatgct ttttcatcct catatctaac gttttcatac ctcttaaaat
    20281 catccatgca ctcattggtg gtaaagatgc tcctgtaaaa caacataaac ttggccatct
    20341 aactaaatct attaattctt ttgaacctgc tactgctccc cctaaagtat ccccatgtcc
    20401 atttataaac ttagtaagac tatgtattac taaatctgca cctaaatcta ttggtctttg
    20461 cacaattggt gtaccaaaag tactatcaac tatggctaaa cttttatttt catgtgcaat
    20521 ttctgctatt gtttttatat ctactaagtc taatgatgga tttaaaggac tttctatata
    20581 aactatctta gtatttggtt tcattgcatc ttttacattt tgtggattta ctgcatctac
    20641 aaatgtaact tcaactccaa atttagataa caactcagac atgaacaatt tagtgtgtga
    20701 gaatattcca tctgaactaa ctatatggtc accacttttt accaatgcta atatagtaga
    20761 tgttattgcc cccatccctg atgtactaga tatggcactt tctgcatttt caagcatagc
    20821 caacttagcc tctaactctg taagagtagg attacactct cttgtataca catatcccca
    20881 tgtcatacac aactcttcaa aatgctctac tgtctttgca gcaaatgttg ctgtttgaca
    20941 tattggtgta gccaatgcac ctgttgttgg gtctgcacta tgtccccaat gtattatttt
    21001 tgtctctaaa tcttgattaa tattgttact cattttaatt tcctcccatt ttgacttatt
    21061 tatacaacgt tttccctgcc aaatttcatc aatttagctt agtattaata cattttttca
    21121 agtatacttt tttatttgtt ataatttcta aaaatatgac acttattttg tctttttaca
    21181 gttctaatta taaactctat agtaactata gagtcaatat gtttttgata ttttttttac
    21241 aatatttaaa atagtcagta caaaaatagg tatatcttag aataactaaa gtcatcctaa
    21301 gatataccta tttttatatc acaatataaa taattacata taagcattag tttttttatc
    21361 ataaatagat ttttcaagat ttatcgtctt ttcataaata tattatagtt aaatatttta
    21421 atattaccta acatctacaa taaaatatac tcttaaaaca aaattatata attaaaaata
    21481 ctctataaaa gttaaaaagt ttttctattc actacatcta aaaataatta gatagaggta
    21541 taaatgtatt tagtttattt atacctctag tcttttataa attatatctc tactaaattg
    21601 attttttagc acgaataagt gtgctcttgg agataccagt catcttcaca acctctctat
    21661 atggcttaga atttaataat cccaatgcat attgtatctg ttgattgctg tatttctttg
    21721 gcctaccatc tttaaagtcc tttttctttt tagcaaattc ttttcctaat tgagtacgtt
    21781 ctattatcat atttctttca agttctgcaa ctgcaagtaa tgtagtcaaa aagaatttac
    21841 ccattgaagt attttctaaa agacctatat ttaaaatatg aacacttact tctttagcaa
    21901 ataaaccttc tattagttgt atcccttctg atgtacttct agctagtcta tccagcttag
    21961 ttgcaacaag caagtctccc ttttttagag atgatataat tttttcaaat ttaggcctgt
    22021 ttgtagtagt gcctgtataa gtttccttta ttattttagc atcattatat ttattcaaaa
    22081 tttcttgctg ttgctgatac aaactgttgt tgtctgtttg attttttgta cttacacggc
    22141 aatatccata tataataatc atcacttcct aacataaaga ttttgtacag aagttaccca
    22201 gaagcttgtt tttacttatc tttatccatt ttactaaaac gatttttggg tgtcaatact
    22261 gttaactttt gacaccataa taattccttt ataatttgtt tgactatgtc aaaaattacc
    22321 aatataatga aattaatgag tttatacaaa ttggaggaaa taatatgaat agtctgttaa
    22381 aagaagcaat agatgaatta aaaaacatcc attgcgggga agttttttta ttaaaagaat
    22441 tgttcaaagg ctatgaatgg aacaggttgg agatgaatat cagattgaaa ttaggaaaac
    22501 tttttttgta tgaagtggta actaatccca atttaaatgt agaggtcatc aataaaaatt
    22561 ctgcttatca gcaaatgtat aaaaaaaaat agtgacttaa atgtatataa tagtataatc
    22621 ttcttattga ttcaaaagtt aaaactttta atacaaaaca atcattctat tttaacattt
    22681 atcaatatgt ctaaatttat ataattaaca catacgaata gtattgttta aatcacatta
    22741 tttaagatag ataaatctta accctactcc agcataaata ctaaaatagg gcttagaatt
    22801 atctatattt ttatcccata aaatgcttag ctcttcctct tgattgaaca tatatatctg
    22861 gtgaaataca atagaagcgt aaatttatga aatttccaaa gtcagaaaac ttcagttcga
    22921 acttaggtat accaaaactt tcaggtgcta tttcgtcaac gccaaaatag atgataattc
    22981 cattatctgt aagataaaag gcttggtctt caggtataac taactcaaca ttagaataat
    23041 acatttctct attttcattt atttgagatt taatgtaatc agtaattata ttaatataat
    23101 caactccatc tctaaaaatg tcattaagta aaagttcttt tccagttgtc aaatctatat
    23161 tgtaattatt caatgtattg tatttaggcc catagttatt agcaaatccc ataaggttta
    23221 atatcatact cagtacataa tttttattaa aagtaactgc gtaactggta gatacccaat
    23281 aactttgttt tggaagtgaa ttttgagaag ctgcgttatt atattctcgt tcttcttcct
    23341 gtaagccatt tttaaaatta tttacagtca ttcttacagc attgtttatg ttatttaaaa
    23401 taactgggtt tacatatgtg gattgatttc taatattgta tataacagga tactttacat
    23461 catatgaaaa atacttactt ctttcaaaat cttcattaaa ttgtacatat aatatattat
    23521 atgtacaggt atttgtagat tgataatttt ccattaaaat cctccccctt ttataaaaaa
    23581 atcagataca atataatatg cttaaaacag taataatgtt aatagtaaat gtaaagaata
    23641 gaaagtatct aaaacagcaa aaaatgacta attttgtggt ataatacata ggattgagag
    23701 ttattttata ggaggaaaaa tatgttatta ttagcagata aatggaaaga ttatgaactt
    23761 atagatatgg gaaatggaga aaaattagaa cgttggggaa attatgtact tagaagacca
    23821 gacccacagg ttgtatggcc aatggaatct gaatgggctt tatggaaaaa ccctcatggg
    23881 cattatcaca gaagcaataa aggtggaggt caatgggaac acaaaaaaag atatccggaa
    23941 caatggacta taaattataa aaacttaaag tttcacatta aaccaactgg atttaaacat
    24001 actggattat tcccagagca agcagctaac tgggattgga tgatagagaa aataaagaaa
    24061 gctaacagac ctataaaagt gttgaatctt tttgcatata caggtggagc tactgtagca
    24121 tgtgcatctg ctggtgcaga agtatgccat gttgatgctg caaaaggtat ggttaattgg
    24181 gcaaaagaaa atgtagaatt atcaggtctt aaaaatcaaa cagtaagatt tatagtggat
    24241 gatgttgtaa agtttgttga aagagaaata agaagaggta aaaaatatga tgcaataata
    24301 atggaccctc cttcttatgg aagaggtcct aaaggagaag tatggcaaat agaagaaaag
    24361 ttatacagtt ttgtggattt atgtatggga gttttatcag ataacccttt atttttcttg
    24421 attaattctt atacaacagg attttctcca atagttcttg aaaatgtatt gtctacaaca
    24481 ataggtaaaa agacaaatac aggagatata tacggtggcg aattaggaat accagcttcc
    24541 agagatggta agatacttcc ttgtggtata tttggaagat gggagaaaaa ataatgataa
    24601 aagtcatata tgaagataac catttattag ttgtagaaaa accagttaac atattatctc
    24661 aaggagataa tactaatgat aaagatatgg taaatctttt aaaagactat attaaaaaaa
    24721 agtataacaa acctggaaat gtatatattg gtttaataca tagattggat agaccagtag
    24781 gtggagttat ggtttttgca aaaacatcaa aagcagcatc tagattgtct gaacaggtaa
    24841 gaagtaagac tttcaaaaaa acttatctag ctgttgttca tggcaagata aaatctaact
    24901 cagatatact aaaggatttt ttatataaaa ataaaaaaac taatatggta actgtagtga
    24961 aaaaaaacca taaagatgcc aaagaagctg agcttaacta taatgtatta gattttaaag
    25021 aaaatttaag tttagttcaa gttaatttaa agactggtcg ttctcatcaa ataagagttc
    25081 aatttgcaag cagaaaacat ccattatacg gagaccaaag atatggcaaa gaaataaata
    25141 agattggaca acaaatatct ctttggtcca ataagataga aatttgccat cctactacaa
    25201 aagaaaaaat ggaatttaca tgtcatccac ctgacaaata tccatggaat atattttaaa
    25261 tctcaaatga ggtgattata tgagtaaaaa attttcaata ggagaaatgt ctaaacttca
    25321 taaaataagc atacagacac taagatacta tgaccaaata ggtttattta aacctgttca
    25381 tgttgaccaa gaaagcaatt acagatatta ctcaattgac caattttcac aattagatat
    25441 tataaaatat ttaaaatatt taggtatgcc tttaaaagaa attaagtcaa agcttgatga
    25501 aaataatgaa gaaattctaa agttactaac aaataaaatt aatatagtag atgaaaaaat
    25561 taaagaatta gagcttataa aaaaggtact aagcacaaag aaagaaacta tggaaaaagg
    25621 tatgaaagta gatgaagttg gtaaaatagt gagaaaacat attccacaaa gaagtatctt
    25681 atctatagaa tatgaagaag gattaagtag tgaagaaact atggaacttt ccagaagaca
    25741 aatagcaaat atattagaag ataatgtatc tgtgttctat ggtggagtaa gtgggcttgt
    25801 atctatagat aaatttttaa aagaagataa agttacttat gacaatagtt ttgttactgt
    25861 tgaaagagac ttatttgata taaaatcaca aaaacatata acagaaattt catctggtga
    25921 ttttatttgt atgacatata aagggcctta caaagaaaat tataccgctt ttaagaagct
    25981 ggttgattat gttgaatata aaagaatacc tattgaaaat tatatatatg aaatacctat
    26041 aatagaccct ctatctacaa acaacgaaga ggaacttcta acagaaattc aaatacaagt
    26101 taaaaagaga gaaattgact taagatgtct agtgtagtaa tctagtttac ttacaaatct
    26161 taatatagtt gtctattatc aacatatgat ttttctagat agtgattcct aacagtgaga
    26221 ttttcaaaaa ataggaggga acatgcgtaa tgctaattcc ctcctatttt taattacacc
    26281 atgtcatttt aattaagcca tgtcatcacg aagtgcatca ataatatttt ctttttttat
    26341 cttattaata gtatacaaca ttgtaataaa tactataaat aaaacactaa atatactaat
    26401 tcctatacta aaccatggaa tcacaaaatc gatatcatct gtaaccatta ctttatgaat
    26461 tagccatgaa gaaattactg ctataggaag tccaaagaac aatgctctca taccataaaa
    26521 agcacactca aaattcatca tcttttggaa atcatgctca gacattccta cagaacgaag
    26581 catggcaagc tctctacgac gtaacttaat attagttgaa attgtattaa acacattggc
    26641 aatcgaaatc agagaaatca taattatgaa agtataagca aaaacattgg caataaaaat
    26701 gtaattacgg ttctcatcca tcattccata catattgtac agattgtact cattcgtaac
    26761 tccctcactc tcaataattt gctccatttc tgcaactgac tgagtaggat tttttgattg
    26821 aaagcttaaa ctttcaattc ctgtagaaac accaggaatc tcaaacttat ctttagccga
    26881 ataaggagct aatactctga agcttgcttt cttcatatct gagcttctga ttcctggagg
    26941 agaatcaggt ggcaccactt tgacaaatga aatatttaca ttttgtctct caggagtgtc
    27001 caacttgcca ttagtttcag gagtgaggct aacattcata gaagaactgg taaaaatatc
    27061 aataatatta ttatcctttg ttaccataga aacagcaatc agttttgcat tttgtccagt
    27121 atattcttct gttggcaatc caagactttt aataatattc agataagttc tatcatcaag
    27181 aaattgcata tctactggta attcaagcat ttcactcata gaatgtgaac cctcatagtt
    27241 caaataatcg tctgtaagct cacttacttt ggcaatacat gaataggtca taagtttctg
    27301 atacaaacct tcgtaaacac cacttgcagt ttttagctta tcataaagtt tcaacatttc
    27361 gttgtctttc ataccttgtg tagaaaaaat aatatcctgg tcagtaacct ctgacctctg
    27421 atttgacatc tgttttaggt ctgctactaa agcacttgta gatataaata gaactatgct
    27481 taaaacaagc gacaatacaa tacttcgata acgtttcttg tttcttttaa agtttttcat
    27541 agcaagaact ccctcaagac cataaactcg ctgtgttagt tttgatgtct tcacagattt
    27601 agattcaatt tttacctcat ttgtctgacg aatacactcc atcactggtg tgttggcagc
    27661 ctttcttgct ggtatgtaag cagaaattaa gattgtaacc atacttacca ctgctgcaac
    27721 gacgattgca gggatagaca gtgttaatgt caacggaacg tttacataga gaatatttgc
    27781 aaagttttta gctatgacag aaatcacaag tccaatactg actataccga ttgcaacacc
    27841 aatcggtata ccaatcgcac caatgcaaat tccttcaaat agcaccgaat ttcgtaactg
    27901 ttttgctgta gctccaacag atgaaagtat tccaaattga tgtgttcgct cgttcaatga
    27961 catgttaaac gaattatata ttagaaaaat tgagcctagc atgattatgg caagcacaat
    28021 gccaccaacc gagtacaaaa acatattgaa caaattgtta tcagaaacac ctatgaagcg
    28081 caacacattg tcattgaaaa tgtaagcatg gtctccagtg ctacttgtgt aagagcgaat
    28141 ctgatgtgga tttttcaatg tgacaaaaag gctaagactg tcagtcttga cttgtttatc
    28201 ttctttggtt atcaaggtat atcctggtgc agaagattct tcaaatacag gctttctaca
    28261 gatacccaca actgtataag atttcttatc ctgtggcaca aaaacttcac tcccagcagt
    28321 gtatggggca ctctgcccaa gcttttcatt ttcactcatt cgtttcccta catccaaaga
    28381 aatagtatca cctactgcaa acttgacacc accatctgtc gcaactttac ttgaaacaag
    28441 aatctctcta ctattttcag gtaatcttcc agaaattaaa gttataggca aagtatcaaa
    28501 agcttctttg ctaaatccag caataaaaag atatggttta tctggatttc tgttaccttc
    28561 aagtgttgca tagccaatat tttcaaatgt tgcagtattt gcaacttcat cattgacttt
    28621 ctgttcctgt acaaaagaag aatcaacatc caaaaactgg acatgccaat caccatattt
    28681 tataatggct ccatttgtca tatagttcat caaagaaact ccaaaggtag taactgctgt
    28741 aatcatagca gctgaaagaa caactcctat aattgttaca agagttcgag tgcgattctt
    28801 tttcaatcct tgcagagcaa ctttgttaaa aatgttcatt aacggttcac cttcctctca
    28861 tcacgcacta cttttccatc tgaaatacag ataattctat ctgcctgtaa agcaatgttt
    28921 tcatcatgtg tgacaataat gagagtttgg tgatatttac tatgactctc tttcagtaac
    28981 tggataatct cttgtccatt tttactgtct aagctacctg taggctcgtc agcaagcata
    29041 actgctggtg cattcatcaa tgcacgacca atggcaacac gttgctgttg acccccagaa
    29101 agttgatttg gtaaatgtgt ttttcgttct tttaaaccaa gtaattctaa caagtcattt
    29161 aaccgttctt ggttgacctt acgcttatcc ataagtatag gcaatgtgat attttccact
    29221 acatttaatg tgggtatcag attgtgaaac tgataaatca atccaacttg tcgcctacga
    29281 aaaatagcaa gtttttcatt gctttgccca tatacatctt gcccctccag atatatcttt
    29341 ccacttgttg gtacgtctac tcctgcaatg gaatgtagta gtgtagattt accagaacca
    29401 gaagaaccaa tgattgcagt aaactctcct ttttcaatag taagtgagac atggtcaagt
    29461 gcagtaacct tattttcatt cttaccataa accttacata aatcttcaat ctttaaaaac
    29521 gccattatat aagtctcctt tcatatttta taaatatatc ataggacttt caacttacat
    29581 ttaagtgaca tttaaatgag agattcgtca ctttggaaaa cgaataacaa atattgcacc
    29641 accttgtggg tgattttgtg cagtaattgt tcctccttgt ctcataataa tcgtcttaca
    29701 aagtgccaat cctattccat aaccagctgt acttgaattt ttcccacgat aaaatctgtt
    29761 aaatagacat ggtaaatctt cttttttaaa gcctgctccg ttgtcatgaa tagtaagttg
    29821 agtaaacaaa aagttgtcct cacaaacgat atgaattttt ccattatcac ccacactttc
    29881 catgcagttt ttaaatatat tttggattgc ttctgaaagc caatttaaat cgcccaaaat
    29941 ctttactcct tttggtatat caatatgtaa agtgatattg tgcagttcca tgggaatcaa
    30001 aagttgatga agtgcagaac taatcaaatt attcacatca atttgttctt tttgaaagac
    30061 tacaatacct gcatctaaac gtgataattt taaaagggaa gtaatcagcc aatccattcg
    30121 tataagcaat tcctctgttt ctcgtatcag ctcttttctc tcatcttcat cagaagtatt
    30181 ctctaacagt gacaaaataa gagttactga tgtaagagga gtgcgaagct ggtgggctat
    30241 gtcagctagt gaatcagcaa ggtgttcttt ttcttttttc agtgcgtaat tttgctctct
    30301 aatacgtaac gtcattttag ttatttcact ttgcagaata gaaagttcac cctcatctga
    30361 ttcagtaata tacagatggt tagcattatg aagcacaaga ttaatttgtt ctgaaatctg
    30421 tgcaatactt ttgtatcgat ttttagtaaa cacaaaaaat gctgttccaa aggtagtggc
    30481 agaaacaata gaaagtattc cagtcatagt actgattgca aatcccaggg ctacagtgag
    30541 aatagtaatt aataagaata aaatagcaaa ttttcgaaac tctctatttc gaaacataac
    30601 ttccccccaa tctataccct gtcccacgaa cagtcaaaat tatttgcgga cttgaagggt
    30661 catcttctat cttctctcgc aagcgtttga tgtacacagt caatgtgttg tcattaacaa
    30721 actcacctgc tgcatcccac aattcatcaa gtaatctcac ccttgtaata atacttttag
    30781 gattgctaat aaacaccaat aacaaacggt attctaaagc tgaaagaaaa acctcattgc
    30841 cattcttttt cacaacaccg gttgccgtat ctacataaag cccacaaatt tcaaaagttg
    30901 acccaaaacg cccacttttt cgtagtgctg ttctaattcg cgcaatcagt tcacgtggac
    30961 gaaaaggttt agtaatataa tcatctgctc ccatattcag cccagtgaca acacttgatt
    31021 catcaccaga agctgtcaaa aatataacag gaatgtcttg ctcttctttg atttctgtgc
    31081 aaatcgtaaa cccatttcca tcaggtaaag aaatatcaat aagtgccaaa tcaaatttat
    31141 tcttagcaat ctctacaagt gcttcacttc ttgtggatac atgggttact ataaaccctt
    31201 ctgaattaag caaaagaata agatttttgg caattgcttt atcatcctca actaaaaata
    31261 tccgtttcat ttactttcat cctcctttat tttattagtt ttatatattc cagcagagca
    31321 ataatacact actattttac ttattattac acaattttca ttagtttctt ctgttcttat
    31381 gtaatctatg cataatacct ccattgattt attatatttc ttataacaga aatacacaag
    31441 ataggacaaa aaacataatg taagtgcaac tattttttaa ttcactaact tataaacaca
    31501 tattccaatg cacgccacct ccctgatgat gtacttatca atctacatgt ttactatggt
    31561 actcccatgt tagtatacac tttataatca atacttgaaa agctaagctc gatgttttat
    31621 tatatactta tatatacctt ataatataag gtatattata atataaagta ggtgaatcta
    31681 tggacttgaa taaagaagta cttaaaggtc atattgatac tttaatatta tcaatactaa
    31741 ataaagggga ttcatatggt tatgaaatag caaaaacagt tagagatgaa acaacctttg
    31801 aattaaagga aagtactctc tatgtatctc ttaaaagact agaatctaag ggtctaataa
    31861 aatcatattg gggaagtgac caaggacttg gaagtagaag aaagtattac aatataacag
    31921 atgaaggaaa agaaaattta gaattaaaga ttcaagagtg gaactttata caaagcgtta
    31981 tgaacaaatt tctaaagaga ggggaataga tttatgaaaa taatagaaga gtatttagaa
    32041 gcactatata aaaatgatac tagcaaagaa attcaagatt taaaagaaga attaaaagag
    32101 catctaatag attccactaa tgaatttatt gagaatggat atgacataaa tgaagcacaa
    32161 aataaggcaa tagaaagatt tgatggcagt aatggagtat cagtagaatt acgttcaatc
    32221 tttaaacaaa aaatagaacc taaaaaagta ctattacgaa aattatcaaa ttcaagaatt
    32281 gtatttttaa atttatttgg atggcttctt gcagctactg catatacaat aaatagaggt
    32341 tttggtcata tagctccacc atggctaatt atacttttaa taatatcaat agttatatta
    32401 ataatcttaa ctattataat taagagatta aaaaaggaaa taaaaaatga ttacaatata
    32461 tagttataca gttatattgt aatatattat ttcttcttat aaacatatat tttgaaagga
    32521 gctttttata tggtcaaaaa aattctatta gaaaaatttc ttgatacctc aggagtttca
    32581 tatatcaatg aaaaattgaa tctatttaag gatactaagt ggatttcatt tggcacctat
    32641 ataatgaccg ttgatagttt ttgttatagg gaggattcaa agcactataa cccaataatg
    32701 tctacctttg attatgaatc agagattttt atagttaatg ccttatacaa ggaaaatatc
    32761 tattctatta aaagtaaaaa actctatagg aatgtatcta aaggagataa gataattgtt
    32821 acaattaaat actcttatga taaatatgaa aatcttatac atgaagaaat tcatgactat
    32881 catctctatc ctctatctct tgattaaaat aaaaggctat agcctctata gctaacacct
    32941 ttaatttaaa atactttgaa tactatataa ttttcataaa agactgtaaa taaactcgta
    33001 tagaatataa aatttatcta cagtcttttg ttatacaaaa tttataatct cttatagaac
    33061 aaatttacca cttttcaaac ttattacttt tactcagcta tattataaac ttttaattta
    33121 tctttgttta aatgtttctt cttaacttct actaaagctt ttagtgtatc caaagatgtc
    33181 attatttcag tagaaaattc aatagctgtt cttctcatct taaaaccatc tcttgtagag
    33241 tcatttcctt tagttggtgt atttattact atatcaactt gtttatttct tatagcatct
    33301 agtatatttg gtctaggttc ttctactcta tttactgttt cagcctctat tccattttct
    33361 cttaaactct tagctgttcc ttctgtagca acaaaagtat accctaattc tttcatatct
    33421 tttgctatct ctataaactc atctttatca tgattattta cagtagcaag tactactcct
    33481 ctttcatcag acatatgtct acctgctgac aagaaccctt tatataaggc ttcttctaaa
    33541 ttttctccta cacctagaac ttctcctgta gacttcattt caggacctag acttacttca
    33601 actttagata atttagacat tgagaatact ggtactttta ctgatactaa ttttggctct
    33661 ttgtaaactc ctgtaccata tcctaaatcc tttaatttag ctcctaacat acacttagta
    33721 gctaagtcaa ctattggtac tccacttact ttacttatat atggaactgt tctactagct
    33781 ctagggttaa cctcaattat atataattca ttttgaaatt ctataaactg tatattaacc
    33841 ataccaagca cattaaggtc taaagctatt tttttagtgt attctactat cttttctttt
    33901 atttcatcag atatattttg acttggatac attgttgtac tatctccaga gtgaactcca
    33961 gctctttcta aatgctccat tataccaggt atcaatatat cttccttatc acatatcgca
    34021 tctacttcta tttctctacc agttagatat ttgtctataa gtactggatt tttgtggtct
    34081 ctagcaaacg catcatccaa atactgaatt aatttttctt cattataagt tatttccatt
    34141 ccttgaccac caagtacata agatggtcta actaatactg gataacctaa ctccttagca
    34201 atctcaactc cctcattagt tgtccataca ccttttcctt ttggtctatt tatatctagt
    34261 ttttctaaca agtcatcaaa cttctctcta tcctctgctg catctatatc cctaaagtct
    34321 gttcctaata taggtatatt tttctcatgt aagaacttag ctaattttat agctgtctgt
    34381 ccaccaaact gtagtattac accttctggt ttttcttttt ctattatatt taaaacttct
    34441 tcttctgtta atggttcaaa atacagctta tctgatgtat caaaatctgt acttactgtc
    34501 tctgggttat tatttacaat tatagtctca attcccattt ttcttaaaga ttttacacag
    34561 tgaactgaac aatagtcaaa ctctatacct tgacctattc ttattggtcc agagcccaat
    34621 actattacct ttctcttatc actaactact acctcatcat attgttcata agttgaatag
    34681 taataaggtg atagagcttc aaattctcct ccacatgtat caaccatttt ataaactggt
    34741 tgtatattat ataaacttct caattcatat aatttttctg ggcttatctt cattaagtca
    34801 gatatacctt tatcagaaaa tccctttttc tttaattcat gtaaatattc tttatctaag
    34861 tcttctattt ttagaacttt taatttttct tcttgttcta ctatccattt aaatttattt
    34921 ataaaccact tatcaacacc agttatttct gatatcattt ctactttata gcctcttctt
    34981 atcatttcag ctaagtcaaa tagtctctca tcatcaggta ctactactct agcctttaat
    35041 tcttctaatg ttctaccttc tgatggagca tgaaccaatg aatattttcc tgtttcaagt
    35101 gatcttatcc ctttaagaat agcagcctca aagttacttc ctatactcat gatttcacca
    35161 gttgccatca tcttagttcc taattttcta tttgcatgtc taaatttatc aaatggccac
    35221 tttggtattt ttactaccac ataatctaat gttggctcaa aacaagcata agttttttta
    35281 gttactgcat tttctatctc atccaaagtg taacctaaag ctatcttagc tgctactttt
    35341 gctattgggt atcctgtagc ttttgatgct agggctgatg acctactaac tcttgggttt
    35401 atttctatta tcgcatattc taaactatgt gggtttaaag ctatttgaac gttacatcca
    35461 ccttgaacct ctattgcatt tattatgtct attgatgcct ttctaagtag ttgatactct
    35521 tcatcactta gagtttgact tggagcaaca actatactgt ctcctgtatg aactccaact
    35581 gggtctacat tttccatatt acatacagtt atacagtttc catttccatc tctcattact
    35641 tcatattcta tttctttcca accttttata ctcttttcaa gaagaacttg tccaacagga
    35701 cttagttgta gtccatgtga gagtatttct cttaattctt cctcagtatc agctatacct
    35761 ccaccagttc cacctagtgt gtatgctggt cttacaacta ctggatatcc tatcttattg
    35821 gcaaactcaa gacctgcttc taaatttgta actatatcac taactattac tggttgattt
    35881 atttctttca ttacttctct aaataaatct ctatcttcgc ccttttttat agactctata
    35941 gatgttccta taacttttac attatattta tctaatatac cagcatcatg taattctaca
    36001 gctaagttta atcctgtctg accacccata cctgcaagta aactgtctgg tctttctttt
    36061 tctattattt tttctataaa ttctattgtt aatggttcta tatatatttt atctgcaatt
    36121 tctttatcag tcattatagt agctgggtta ctatttacta atacaacttc aattccctct
    36181 tcttttaatg cttggcaggc ctgtgttcct gaatagtcaa actctgcagc ttgacctatt
    36241 attattggcc ctgatcctaa tactaaagtt ttctttatac tatctaattt aggcataact
    36301 ctttctcctt atctaaacaa ttttcacagt tcttatcttc atcatctata caatcaactt
    36361 taattacttt atttcatctt aaattcaaat ttactcatca atacatctat gttaattaaa
    36421 ccaaactttt tgatttaagt tattcttgtt ttttagttag ctattctaat gttatttatt
    36481 ttactaattc taaaaattta tcaaaaatat agtcattatc ttttggtcct gggcaagctt
    36541 caggatggta ttgaacactg tatacatcta gcttctcatg tctcattcct tcaacagtat
    36601 tatcatttaa atttatatgt gtcactttca tttgttcagg aacttctgaa acataataac
    36661 catgattttg agaagttata aatacttttc cctcttctaa atctttaact ggatgatttc
    36721 cacctctatg tccaaattta agtttagcag tttttcctcc aagagctaaa gctaaaagtt
    36781 gatgccctaa gcatattcca actataggtt ttttaccaat aagagctttt atattttcta
    36841 taacatcttc taggtcttct gggtctccag gtccatttga caagaatatt aagtcagggt
    36901 ttatactcag tacatcttct ggagatgttg tagctggaaa tattgttata tcacatccac
    36961 gagctataaa tgatcttaat atatttcttt ttactccaaa atccataaca gccacttttg
    37021 gaccatttcc ttttatatgc tctacttctt ttcttgtaac agttctaact gcttctgtat
    37081 ttgaaaactt atccaattta tgtttaacat cttctaattt actgtcttct aatgtaatta
    37141 tacctttcat tgttccatta tttctaagta tcttagtcaa agctcttgta tctattcctt
    37201 caagtccgat aactttattt tgctttaaat attggtctat atcaatttca catctaaaat
    37261 tacttggaga atcacttttc tcccttacta tgaatccctt tacttgaact ttttcagact
    37321 ctgctgatga taaatttata ccataatttc ctacaagagg ataagtcata gtaactattt
    37381 gaccatagta agaagggtct gttaaaactt caccatatcc tatcatcgaa gtattaaata
    37441 ctacttctcc tacactttct tctaaatatc caaatgcctt tcctataaaa actgttccat
    37501 cttctaatat taaccttgct ttcatactac agtacctcct ataattctct ttaggcaaac
    37561 taaagaacat taactaaata taagctttta aatatctata tctatttttt acttttacat
    37621 tctagtttta tacttattta catttatttt ttatcttttt ctattatact acaaatttct
    37681 taaaaaaaag aagttatctc aaaagtaaaa actcctagaa aaaataacct ctttttttga
    37741 tttaataatt aaattcttaa actatacaat cagcttaatt tctattcagc tatattgtaa
    37801 acttttaact catctttatt taaatgtttc ttcttaactt ctactaaagc ttttagtgta
    37861 tccaaagatg tcattatttc agtagaaaat tcaatagctg ttcttcttat cttaaaccca
    37921 tctcttgtag agtcatttcc tttagttggt gtatttatta ctatatcaac ttgtttattt
    37981 cttatagcat ctaatatgtt tggtctagat tcttctactc tatttactat atcagcctct
    38041 attccatttt ctcttaaact cttcgctgtt ccttctgtag caacaaaagt atatcctaac
    38101 tctttcatat cttttgctat ctctataaac tcatctttat catgattatt aacagtagca
    38161 agtactactc ctctttcatc agacatatgt ctacctgctg ccaagaatcc tttatataag
    38221 gcttcttcta ggttttctcc tacacctaga acttctcctg tagacttcat ttcaggtcct
    38281 agacttactt caactttaga taatttagac attgagaata ctggcacttt tactgatact
    38341 aattttggct ctttgtaaac ccctgtacca tatcctaaat cctttaattt agctcctaac
    38401 atacacttag tcgctaaatc aactattggt actccactta ctttacttat atatggaact
    38461 gttctactag ctctaggatt aacctcaatt atatacaatt cattttgaaa ttctataaac
    38521 tgtatattaa ccataccaat cacattaagg tctaaagcta tttttttagt atattctact
    38581 atcttttctt ttatttcatc agatatattt tgactcggat acattgttgt actatctcca
    38641 gagtgaactc cagctctttc taaatgctcc attataccag gtattaatat atcttcttta
    38701 tcacatatag catctacttc tatttctcta ccagttagat atttgtctat aagcactgga
    38761 tttttatggt ctctatcaaa cgcatcttgt aggtattgag ataatttttc ttcgttataa
    38821 gttatttcca taccttgacc accaagtaca taagatggtc taactagtac tggataacct
    38881 aattcattag caacttcaat accttcgtta agtgaccata caccctttcc ttttggtcta
    38941 tttatatcta gtctctctag taaatcatca aatttttctc tatcttctgc tgcatctata
    39001 tcatcaaaat ctgttcccag tataggtata tttttttcat gtaaaaactt agccaatttt
    39061 atagctgttt gtccaccaaa ctgtagtatt acaccttctg gcttttcttt ttctattata
    39121 cttaaaactt cttcttctgt taatggttca aaatacagct tatctgatgt atcaaaatct
    39181 gtacttacag tttcagggtt attgtttatg attatagttt ctatgtccat ttttcttaaa
    39241 gattttacac agtgaactga acaataatca aattctattc cttgacctat tcttattgga
    39301 ccagaaccta atactatcac ctttttctta tcactaacaa ctacctcatc atattgctca
    39361 taagttgaat aatagtaagg agatatagca tctatctcac ctgcacaagt atcaaccatt
    39421 ttatatgctg gtttgatatt gtatagactt ctcaattcat ataatttttc tggacttatc
    39481 ttcattaagt cagatatacc tttatcagag aatccctttt tctttaattc aagaagatag
    39541 tctttagtta aatcttcaat tttcataact tttaattttt cttcttgctc tactatccac
    39601 ttaaatttat ttataaacca tttatctata cctgtaattt gttctatcat ttctacttta
    39661 tagcctcttc ttatcatttc agctaagtca aatagtctct catcatcagg tactacaact
    39721 cttttcttta attcctcaat acttctctcc tcagatggtg tgtgaactaa tgaatatttt
    39781 cctgtttcaa gtgatcttat ccctttaaga atagcagcct caaagttact tcctatactc
    39841 ataatttcac cagttgccat catcttagtt cctaattttc tatttgcttt tttaaattta
    39901 tcaaaaggcc actttggtat ttttgccacc acataatcta tagttggttc aaaacaagca
    39961 taagtttttt tagttactgc attttctatc tcatctaaag tataacctaa agctattttt
    40021 gacgaaactt tagctattgg gtatcctgta gcttttgatg ctaaagctga tgacctacta
    40081 actcttggat ttatctctat aactgcatat tcaaaactat gtgggttaag tgcaaattga
    40141 acgttacaac caccttgaac ttctactgca tttaatatat ctaaagatgc ttttttaagc
    40201 attgcacatt ctttgttact taaagtttga gttggagcaa caactatact gtctcctgta
    40261 tgaactccga ctgggtctat attttccata ttacatacaa ctatacaatt tccctttgaa
    40321 tctcttatta cctcatattc tatttctttc caacctttta tactcttttc aattaatact
    40381 tgtccaacag gacttaattg tagaccatgt gaaagtattt ctcttaattc ttcctcatta
    40441 tcagctatac caccaccagt tccacctagt gtgtatgctg gtcttacaac tactggataa
    40501 ccaattgtaa gtgcataatc aagacctgct tgtaaatctg taactatatc actaactatt
    40561 acaggctgat ttatttcttt cattacttct ctaaatatat ctctatcttc accttttttg
    40621 atagattcta ctgaagtacc tattattttt actccatatt tgtctagtat acctttttca
    40681 taaagttcaa ctgctaagtt tagccctgtt tgtccaccca tacctgcaag taaactgtct
    40741 ggtctttctt tttctattat tttttctata aattctattg ttaatggttc tatatatatt
    40801 ttatctgcca cttctttatc agtcattata gtagctggat tactatttat taatacaact
    40861 tcaatccctt cctctttcaa agattggcaa gcttgtgttc ctgaatagtc aaactctgca
    40921 gcttgaccta ttattattgg tcctgaccct aatactaaag ttttctttat actatctaat
    40981 ttaggcataa atttttctcc ttatctaaat ggtttttact atctgtaaat aatactaaat
    41041 ctatattaat gctaagaatt tattaaatat acaatctaaa tcttcttttg ttgtgtattc
    41101 tggaatgtat tgaacactat atattggtaa ttccttatgt ctcattcctt ctatagtatt
    41161 gtcatttaaa tttatgtgag ttatctctgt attttctggc atttctgaaa cattatatcc
    41221 atgattttga gaagttataa atacctttcc agtttctaaa catttaactg ggtgattgcc
    41281 tcctctatgt ccaaatacaa gctttgaagt ctttcctcct aatgccaatg ctaaaatttg
    41341 atgtcctaaa cctattcctg tcaatggctt tttgcctatt aattttttta tattttcaac
    41401 tatatcattt atatcctctg gatttcctgg tccattagat aataagatta agtcaggatt
    41461 tatatttaaa atttcttcta ctgtagtcat tgctggtact atagttatat cacagttaca
    41521 agctttaaga gactctagta cactattttt aacaccaaag tctataacta caaccttagc
    41581 accagtacct tttatatttt caatttcttt tcttgtaaca ttttttactg aatctttgtt
    41641 acaatgctct tctaatttag actgaacatc ttgtaatgat acattttcta aagttataat
    41701 tcctctctta gttccattgt ttctaataat tttagttaat gctcttgtat ctattccttc
    41761 aagtcctatt accttatttt gctttaggta tgtgtctata tccatttcac atctaaaatt
    41821 atttgggtta tcactttttt ctcttacaat aagtcctttt acttgcacct tatttgattc
    41881 aacatcttct aaatttattc catagtttcc tataagtgga taagtcatag taactatttg
    41941 tccataataa gttgcatctg tcaatagttc tccataccca gccattgaag tattaaatac
    42001 aacttctcca atagtttcct ttagatatcc aaatgctttt ccttcaaata ctgttccatc
    42061 ttctaaaatt aatttcccct tcatgtaagc tacctcccaa atattttatc taagcatttt
    42121 ctatttctct tacaatatta atagctgatt ctcttggatt ttctgctttt gttataggtc
    42181 ttccaacaac taaataatga gcaccttttt ttattgcatc tgatggagtt acaactcttt
    42241 tttggtctcc aacctctgct gatttaggtc ttattcctgg acaaactgtt ataaaatcag
    42301 ttccacatgc ttctactatt ttatctactt cttgtgctga acaaacaacc ccatctattc
    42361 ctgattcttt tgccagttta gctctcttta tagctaactc ttctgttgtc atattacatc
    42421 ctatgtcctg taaatcttgg ttgcttaatg aagttagaac cgttactcca actactaatg
    42481 gcttttctat atttaatctt tctgcttctt ccctagcaat tagtgcacat tgtctcattc
    42541 cttccatatc tgatacatgt attgtcatta accaaacatt atctctcact gcagctctca
    42601 ctgcactttt catagtattt ggtatatcat ggaatttaag gtctaaaaat attttttttc
    42661 ctttttcttt aagataatct attgttttac cttttgtggc aacgtattgc tctagcccca
    42721 ccttaaatat gtctacacta tcttctaatc tgtctattag ttctttagct ttatcaaatt
    42781 cgtcagtatc aattgcaact atcaactttt ctttaccatt tatcattttt acatctcctt
    42841 tttaataaag cctaaataat cataaaaaaa tcctcacacc aaaagaggca caaggatttt
    42901 atctatacat aactctattc attagctatg agatatcttt cccagtctct ctggactagt
    42961 ttaaaagact ttattatatt taaattttta ttttatactc atcaaaaacc tctagaagat
    43021 ttactagagg ttatatacta acactatcta taacccttta gtctctctgg actactttaa
    43081 ggaatttagc tgtaatattt ttctttattt atataacttt atgtatcact aggataattt
    43141 aaagttttat cttttttata attaaaccac taattgcttt ttatgtcaat aagtctttga
    43201 attattcttc tttatcaaac aattttttct caaaatatga ttctgcatta tatattacag
    43261 caactggatt atgcccagtt actctatctt tgacaactaa agttgttata ggagcttcca
    43321 tatatttcat ggctaaagta tcgtgaccaa cacataatcc aagaattaca ttaaggtcaa
    43381 ccttagcttc atttaaaaat aaagcttgtc ctattggatt gcacattacc tcatctgaac
    43441 actttgatat tgtttgagat gcatctagtc caattgtact cttaggcatt gctccatttt
    43501 tacatataac agaaaatact tcaaatccat gatgttcaaa tattttttgt actgtttgca
    43561 tttccctttt tagcccaata caaaatgcta tgccaatctt tttataacca cactttttag
    43621 caaactctat aatctctttt agtctagtat attgacaata cccctctgct tcaactaatc
    43681 cagcattata agctattttt ctattctctt cttcttcata aagttgcttt gctttttgtt
    43741 gtagttcaat ttctctagtt ggacatatct tcattaattt atccatattt ccattagaac
    43801 agcctctaag attacatttt ccacacttat acatatccaa tatccccttc caaaatttta
    43861 ttcatcttga gattcctctt tttctaggat tacattttct tcttggattt cttcttctgg
    43921 tttcacatgc tcgactacag ttataaaata tgattttcct attttattcc ttataggaag
    43981 tttttcatat gaaacatcta ttttattttt tatacaagct atatcaaaat gtcttaaggc
    44041 tacacctaag ctaaccttat tcatatcttc acagtgtttc ttaggtctct tttcaaataa
    44101 atgaagacct cgttcatcct tatggaaata ccacggttga gaatttttta cagatggagc
    44161 tcttcttgtc aattctaaga tatctttcca ctttctattc ctatttatct tatttatcat
    44221 atcttttact ggctttctat caggtctagc atatctagtc ctaaacaagt cttcattttt
    44281 tgctggatat ccaaatgcta ttattatata aggattttct attttgtttt catcatcttc
    44341 ttctgttttt attccagcta gataatctaa atcatcttcc tcatcttctt caaaattctt
    44401 attcccaata tctacaaatt ccaagatatc ttctctcttt aagtttcctt ccaaccagca
    44461 agtagctatt cccaatgttg taaggcgcaa aactacacct tctgtagcaa atcctatatt
    44521 ctgtaaatag tcctcacctt tatctgaggt aactactata taatgaggtg cctttacttt
    44581 acattcctta cccatcaaaa aatgtattat atgacctcta tcaacaacat gtgctttaat
    44641 atttaaatct tcatttaagt attctaaatt tttacaactt ctttttattt cttccatcaa
    44701 tggctttttt atcttcttac tggaaaaatt tcttacagat tttctataaa aaatggcttc
    44761 ataaagctcc atttatgccc tcctaactga aatcttctga cgtataaact ctataaattt
    44821 atataaatac tttatatttt ttatagtttt atttcctttt tcctcctgcc ctattttgaa
    44881 attcataaga atatctactc tagtttaata tagcattcaa aaggcttaaa ttctacctta
    44941 tcaatgtcta tacattagca taattttttt atttgatttt ctatgctaat ttaaataaca
    45001 acactagcta ttaacttttt aattaagctt tgccagaaaa atttttttca tttataaata
    45061 tctatctcat tattatatta taagtttttt tcggtattct tgtatagctt ttcactaaaa
    45121 aagtattttt atttctactt taatactatt atcagcaaaa aacatactgc ttaaataagt
    45181 ttgttatcct cataatctgt tttaattaaa atattttttt tatttctcaa tctataaagt
    45241 ataaatattt tgaatatgac ccaaaatcta agtaaagtta taatatttta taagttgttc
    45301 aatattaaaa acataagtaa tgttataata ctttataagt tattcaatat caaaaatata
    45361 ggaggataaa tatgaattta aatagaaatt tagaaaaata cgctgaatta gcaataaaag
    45421 ttggagttaa catacagcca ggtcaaattc tacttataaa gtcaccaata gaatgtgctg
    45481 attttgcaag aaatgcactt aaagaagcat ataaatgtgg tgctaaaaat gtttatatag
    45541 agtggtctga tgaagaaagt actcttatta aatacttata tgctccagat gaagcatttc
    45601 atgagttccc caaatggaca gcagaacaat atgtagatat agcaaaagaa ggtggagcat
    45661 ttttatcagt ctatgcccaa aatccagatt tattaaaaga tgttgaccct gaaagagttg
    45721 caaattttca gaaggcatcc ggaaaagctt taaaagaatg gcgttcttat actctaactg
    45781 ataaatgtaa atggagtata gtctctgtgc caacaaaaga ttgggcaaaa aaggtgtttc
    45841 caaatctatc agaagataaa gcaatagaaa aactgtggga tgctatattt aaatgttcaa
    45901 gagtagatgg tcaagaccct ataaaagcat gggaagaaca taatgaaaat cttaaatcta
    45961 aaatggactt tctaaataaa aataacttta aaactttaaa atataaatcc tcaaaaactg
    46021 atttgactct agacttgcca aagggtcatg tttggctaag tggagcatcc aaagacccta
    46081 atggaataag ttttaatccg aatattccaa ctgaagagat ttttggaatg ccacataagt
    46141 ttaaagtaaa tggaactgtt tatagtacaa aacctcttgt ttatggtgga aatataattg
    46201 acaatttctt cttaacattt aaagatggta aaataataga tttctctgct gaaaagggac
    46261 tagatacact agaaaaactt attgaaacgg atgaaggctc tcattattta ggagaagtcg
    46321 ctcttgttcc atacaactca ccaatatcca atacagatat aattttttat aatactctat
    46381 atgatgaaaa tgcttcttgc cactttgcaa ttggctctgc ttataagact tgtctggaag
    46441 gtggaaacaa tttaaaagat gaagaattag atgaacatgg tgtaaatgac agtttaacac
    46501 atgtagattt tatgattggt tctgctgata tggatattat tggtgagact tatgatggaa
    46561 agcaaataca gatttttaaa aatggtaatt gggcttttta acatataatt ttaactaaaa
    46621 agggctgatg gaagttatac cagacttccc agcccttttt aatgttgatg ataaatattt
    46681 tttatattaa ttaatctcat tattatagat taattcctat tgttttaaac ataagtatct
    46741 aaaacttata tttatattat atataaaata tcctatttag taaaaccttt attttaattt
    46801 acatgctaaa aatttaactt tgctatttca acagcagact ttgcatcctt tgaataataa
    46861 tcagcaccca tttcttcagc gtattcttct gtcaaaactg cacctcctac aaataccttt
    46921 gcatttatct cattatctct cagtgcctga attgtatctt tcatactttg aacagtcgta
    46981 gtcataagtg cacttaatcc tactaatttt atatttctct ttttagcaac ttctactact
    47041 tcttcaatag ggacatcttt acctaaatct ataacctcat atccataatt ttctagcatt
    47101 atttttacta tatttttccc aatatcatgg atatcacctt ttactgtagc aactattatt
    47161 tttcctttag atacattatt gctactttta cttaataaag tttcctttat tgtatttaat
    47221 gaaactttta ccgtttctgc tgattgaatc aattgtggta aaaatatatc tccactatca
    47281 tatctttttc ctactttatc aagagcagga attaaaatct catctaatat ataattttcg
    47341 tccttctctt ttaaaagatt cagtgttata ttttttgctt catcttttaa tcccctttca
    47401 actaaaatat ccaagggcaa atctttgtca atctttgtag atgaatccga tttttttgaa
    47461 ttatttatat tactatattg atttatatag tttatacatc ctttatctgt gttatttaat
    47521 attttaaaag aattgatagc ttccatcata ctatattcat ttggatttat aattgctaaa
    47581 tcaagccctg cccctaaagc taatgttaga aaagaagcat taagtgcttt cctatttgga
    47641 agaccaaatg aaatatttga aacacctaat atagttttta ctcctagtgt ctttaccatt
    47701 gttatagctt ttatagtttc tattgcttct tcttgttgtg ctgaaacagt taatgataaa
    47761 caatctataa atatgtcttt tggttttatc ccataagaaa ctgctctatt aactatcctt
    47821 tttgctattt caaatctttc ttcagccttt ttaggtatcc ccttttcatc aagtgtaagt
    47881 ccaaccacac atgaaccata ctttttaact attggtaata tgctttctaa ggactcctct
    47941 ttgccattta cagagtttac tattgttcta ccattataat atctaagtcc ttgctctaag
    48001 gcctctacat tggaagaatc aacttgtaaa ggtgtatcta ctactgcttg aatctctctt
    48061 attaattttg gcattagttt tttttcatca atctctggaa gaccaacatt cactccaagt
    48121 atttgtgctc cagcatttac ttgttctaag cctaaattta tagcataatc aacattttca
    48181 ttttttaatg cttcttgtag agattttcta cctgtgggat tcaatctttc tccaacaact
    48241 gtaggcgatt gtacttccac aaattttgat ggtgaacaga ctacacattc atcaattttc
    48301 tcgatacatc cctttgtaac agaatttata ttctcagata tttcttttat aaaacttgga
    48361 ttcgtaccac aacatccacc tattatagta gcaccaacct caacgaactt tttaacccct
    48421 tcaaaaaatt cttttgcatc aacatcatat atagcctctc catcaactat atttggaaga
    48481 cctgcatttg cttgaaccat aacagggact gtagctcttg atgctatttt ttcaactatt
    48541 ggtaaaagtt gtttgggtcc taatgaacaa ttaactccta ttgcatctac acctagacct
    48601 tctatagtag ccaccataca ttctggcata catccagtaa aacttcttcc accttcatca
    48661 aatgtcatag tacagaaaat aggtaaatca gaattttctt tagctgctaa cacagctact
    48721 ttagcttcat ataaatccat catagtctct attactatca catcaactcc agacttaacg
    48781 ccttgtagta cttgtctttt aaatatttca tatgccttat caaaacttaa agtacccata
    48841 ggctctagca tttctcctat aggaccaata tctaaagcta catatcttgg tttagtatta
    48901 tctacatttt ctatagcttt tctagccacc aaaacagcat tgtcaatcac ttcttctaca
    48961 gtatacttac tatctaattt tagttcatta catccaaaag tatttgtaag aattacattt
    49021 gaacctgcct ctaagtatgc tgtatgtatt tctataagtt tatctggatt ttgaagacca
    49081 aatacttctg gattttcacc taactttaac cctttttgtt gaagcattgt tcccattgca
    49141 ccatcaaata ttaatatatt atttttcaga tattgtctta tttccacaag agttcgctcc
    49201 ttttctatat gtacagtttc tatagttttt acaattttca cacgactttt ctttcttaga
    49261 tttatttatt cttgtattag aaatacctat aatagccact actgactttc tcggtatcat
    49321 aattccatta gaagtaattg ttagacctat ttgtttttga gcattcaaaa cgtttaatat
    49381 atctatattt ttttctattg gtaaatctcc atatcctggg ctatatcgca tagttataaa
    49441 tttatcttgc tttaaaaaaa ccttttctaa attattttga acaatgtcac acacttcttc
    49501 tattgctgtt gttccacatg aatcaattat tagtcctttt gttaaatttg aataagagtg
    49561 tcgtcttatt tctttttcta tatctatccc tatagtagct gccataatta cacactcatt
    49621 acattctttt aaaagttcat atatatcttt actctctaag gttaagtttg ttccctctaa
    49681 ttctacaata ttatctttat ttattctaat aggataaatc ctagataaat atctaggatt
    49741 tattttattt ttagttatag ctacacattc atcaatagta tatttaaggt ctttatctat
    49801 ctcttgacct ctgtattgta aatatcttaa aacttcactt tgatttatgc ttatatcttc
    49861 tcttccaatt aaattattct ccatatatat ttaaagaaaa tcctttatct aaaacatctt
    49921 ttgcatattc ctttgcccca aataaagata agtctctgta gtttccacat tctacagctg
    49981 tagctgctgg cattttatct gattcaagaa ctttttttaa tgcttcttct aaaccaatct
    50041 taactgtttt ggcatctaca tctccccata gtattaaata aaatcctgtt ctacatccca
    50101 ttggagataa gtctattaca ccatctaatg tatctcttaa ataagtagct aaaagatgct
    50161 ctaaaccgtg catagcagca gttccaaaac tttctacatt tggctgtaaa aatctcaaat
    50221 caaatttagt tactttatct ccctttactc catcaagtac actacacttt cttacaaaag
    50281 gagcttttac ttttgtatgg tctagcttaa aactttctac tttttccatt atttaatcct
    50341 ccattatact ttaaaatttg ttacattaat tctaaaaaaa actcttccta tctattattg
    50401 ataagaagag tatattattt caagtcatct catcgttgtt agcaggacca cctgataaaa
    50461 ggttggtata ggatcactga gctaactctc tcccctaact cttgataagt atcttattaa
    50521 actgacttaa attatatatt acttttttat ttttgtaaac actttattgt tataatattt
    50581 atatcacata atcgtacttt tattttataa ctaagctgat tgtattattg gcagttgtac
    50641 ctcaaataca gtttgatatt tatcgcttga aacagtaatt tctccattat gtaaatttac
    50701 aatatttttt gtaatcgcaa gacccaatcc agaacctcca acatttctat ttctagattt
    50761 ttcaactcta taaaatctat caaatatatt tggtaaatct aacacaggaa ttggttctcc
    50821 ataatttatc acttttacaa ccgccatatc ttcctttttc tctgtgacta tatctacata
    50881 atggccatct tttccatatt tcacagcatt agttataaga ttttcaaatg ctcttaccaa
    50941 tttatttgga tcagctttta caattaattt ttcttcatta aaattaattc tactaaccat
    51001 aaattcttgt ttaaaataaa tttcaaattg agatactact tgacttaata actctactaa
    51061 atttatttct attttgttta attttattgt attattttgc attttagtca attcaaacaa
    51121 atcatttata agaacattta aatttttcga tttttcatat gcaatatcaa cataatacat
    51181 catctgaact tcatctttat acttaccttc ttctatcact cctaaataac ccattatgga
    51241 tgtcaaaggt gtccttaagt catgtgacac atttgttatt aaatctgttt ttgtctgctg
    51301 agcttgtctc tcttcaatag ttatatttct aagttgatgg actatattat tgattttatg
    51361 tgctagttca tcaatatcat ccttttcttt atcctttaat atatctaagt catactcaat
    51421 atctatactt ttttctaagt taccttcatc cattaaattt acagcacttt taatgatttc
    51481 taatttttga ttttttctat tagtctttct tataaaatct acaatttgct tatttctctt
    51541 gtatgatatc atggaataaa ttgctaatga tgtgattgta aataaaaatg gataaaataa
    51601 actctctcct ataagacttc tgaaatctct taactgatat acaagttgat taattacaga
    51661 tatctcacta ttgaaatata ataattttat tatatacata cttgttaaaa taagcattga
    51721 agttaaacca cagcttatta taagatctaa tatgggtcga aacatatgat ttaataaact
    51781 tttatttttc aattttatat cctactcccc atacagtatg aattattttt tctccatctg
    51841 tatatttttc aattttatcc cttattctgc tcatatgaac cattacagta ttattagatt
    51901 ggtaatattt ctctttccat accttttcaa atatttcttc tgtactatat actctgcctt
    51961 tattgtttgc tagtaaatat aatatatcaa attctcttga tgtcaaaatt atttcatttc
    52021 cagctactgt tacactatgt ttttccttat ctataattac tactccaact tctatgacat
    52081 ctttatcttc attattagaa ccattaaaat aatatgctct tctaatcagt gatttcactc
    52141 tcacagtaag ttcaagtggg ttaaatggtt ttgtcaaata atcatctgca ccagtcatta
    52201 tcccttgtat tttatccatg tcttctgatt ttgcacttaa cataagtatt ggtatattca
    52261 aactttctct aacttttcta caaacttcta tcccatccat tttaggcatc attatatcca
    52321 ataccattaa atgaatattt tcatttttta aaatatctag agcctctttt ccatcttcag
    52381 ctttaattac attatatcca ctatttaaaa gatatatctc tataaggtct cttatttcct
    52441 tttcatcatc cacaacaaga actgtattgt tcatttttac acatcctaac ttcatctttg
    52501 tatataactt tactcatata aaattatact atattttagt tagtgtaatc tataaattta
    52561 ttcatttcta aatttccaat ataatacaaa ttatttacac atttatatta tttattttgt
    52621 aaatattctt ctaaagttaa tccttttgaa taaatttcct cggcagcatc cttaccaaca
    52681 tatctaatat gccatggttc atatgctgta cctgttatat cttctttacc ttcaggatat
    52741 cttattataa atccaaactt gtgagcattt tctgccaacc atttagcttc ttttgtactt
    52801 ccaacaaacc atctttctgg atttgttaaa tcaatacaaa gtccagtttg atgctcactg
    52861 taaccaggct ttgctacata tgcatctgcc ttttctcgcc cctgagattt tacccttctt
    52921 gtataagtat ccagttgagt atcataagat ctgtatgctg aagaccctaa aaactgtatt
    52981 ccttctgaat tagcttgctt aactaaatct tcaactgcct ttgcaggttc tcctgccatt
    53041 tgtttttctt cctctgttgc ttcttcaaca aaaggtatgt ttaccttagt aatattttca
    53101 ggagtatagt ttttgctaat tccatttgtc ttgtttacca ataatatatt tgatatacta
    53161 tttgagtgat ttttattttg agaatttgcc tcaacatctt gttctgaatc atgttgagca
    53221 actactattg gatctttatc tttcttaagt aatctctctt tagatataaa tacacttata
    53281 aacactacta aaataatggc tcctattaat ttaaattttt tcataattac atattctcct
    53341 ttttgttatg tttgtctata atgttgattt tagagtataa gacttaagta ttttataagt
    53401 aaatcttaaa taaatcttaa atttaatatt tttaaaggat ggtgataaaa tgtctataca
    53461 aaaacttcta agtaaagctc gtaaggctat acaagatttt gatatgatac aagaaaatga
    53521 taaaattgcg gttggacttt ctggtggaaa agatagttta actctacttc atatacttaa
    53581 aaattatcaa agattttctc cacaaaactt tgaattgata gccataacat taaataccgg
    53641 aggtgttgat aattctccac tagataaatt gtgtaaagaa ataaatgttc ctttttatga
    53701 atttcaaact gacataaaag aaattgtatt tgatataaga caagaaaaga atccttgtgc
    53761 attatgtgct aatttaagaa gaggtgcttt gaacgataat gctaaaaaac ttggatgtaa
    53821 taaagtagct cttggtcatc ataaagatga tgcaattgag acatttttaa tgtctatgtt
    53881 ttatgagggt agagtaaatt gtttttcccc aaagacatac ctagatagac aggatttaac
    53941 agttataaga cctatgattt acatagaaga gtatatgact aagaaaattt caaaagattc
    54001 taactatcct attatcacaa atccatgccc tgcaaatggt catactagga gagagtacat
    54061 aaaaaatctt attgctaatt taaataaaga gatgcctgat tttaaaagaa atgtatttgg
    54121 agctttaaat aattctgaga aattgtttat atgggataaa gagaagataa aaaattttaa
    54181 ataataatta aaatttttaa ata
//
LOCUS       DAEPXF010000007        46339 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain HB27
            SAMN13376389-rid12479423.denovo.008, whole genome shotgun sequence.
ACCESSION   DAEPXF010000007 DAEPXF010000000
VERSION     DAEPXF010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13376389
            Sequence Read Archive: SRR10518432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 46339)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 46339)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001069624.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001069624.1
            Genome Coverage       :: 315x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/15/2021 01:54:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,165
            CDSs (total)                      :: 4,076
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 89
            rRNAs                             :: 10, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 10 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 19 of 69
            Pseudo Genes (incomplete)         :: 44 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 10 of 69
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..46339
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13376389-rid12479423.denovo.008"
                     /strain="HB27"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="China: Hebei"
                     /collection_date="2017"
     gene            complement(404..607)
                     /locus_tag="KRL67_000377"
                     /pseudo
     CDS             complement(404..607)
                     /locus_tag="KRL67_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860778.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA pseudouridine(516) synthase"
     gene            complement(607..936)
                     /locus_tag="KRL67_000378"
     CDS             complement(607..936)
                     /locus_tag="KRL67_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1904 domain-containing protein"
                     /protein_id="HBH1521284.1"
                     /translation="MPQIKIRGINENDICKISEKMIDDLVEAVKCPRDYFEIECIKSV
                     AIRDGKIADVYPFVEVAWFDRGQEVQDIVARIITDSIRNNLDVESMDLAFTVFEKEKY
                     YENGEHF"
     gene            1266..2192
                     /gene="fba"
                     /locus_tag="KRL67_000379"
     CDS             1266..2192
                     /gene="fba"
                     /locus_tag="KRL67_000379"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-1,6-bisphosphate aldolase"
                     /protein_id="HBH1521285.1"
                     /translation="MALITTKEMFKKAYEGGFAIGAFNISDLEQLQGVLKAAKAKNSY
                     VMIQASMSAVKYAGPHTLVEMVKAASDEIGVDVALHLDHGPNMDAIKTCIDAGFSSVM
                     IDGSHFDFEENVRITKEAVEYAHSKGVVVEAELGVLAGTEDDVTSDVHKYTQPAEAVE
                     FVERTGVDSLAIAIGTSHGAFKFKGEAKLRFDILEEIQSKLPGFPIVLHGASAVDQNA
                     VATCNEFGGNIAGAKGVPVDMLRKASSMAVCKINMDTDLRLAMTAAIRKFLAENPKEF
                     DPRKYIGAGRDAIQAVVESKIDDVLGSANSIN"
     gene            complement(2327..3958)
                     /locus_tag="KRL67_000380"
     CDS             complement(2327..3958)
                     /locus_tag="KRL67_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBH1521286.1"
                     /translation="MDNNLIGLIEHVENPAILCKESGEIVYCNHLIDSIFSFLDIKKP
                     RNINELDSNFDKTEILTDSKKKIAFRELRMTAHIYNMKDNNNENNIVYLFEKSLISDR
                     VIEDIIEHIDEVVVVFNKDGVIEKMNTVSDEILPFKRTEVLGRNITDLVRQGLVEEPI
                     ILNMLKVKKKIYRNIVYPDGKLIAYTAVPRWDSKGKLTGGVLTGRDISRVIKLESQIK
                     YSDISEDTEYISQSKIMDNIKKVVKRAAASDSSIFINGESGVGKEIIARTIYKYSSRR
                     DKPFIAINCGAIPNELLESEFFGYEEGSFTGAKKKGKKGLFEEANGGTIFLDEIGELP
                     MQMQKKLLRVIQENTITRIGGSKPIKIDVRYISATNISHEDLRNNLKFRQDLYYRLSV
                     IPVKIPPLRERKEDIVPLVNYFLKLYNEKYNREVEVSPKVIELLEEYSWPGNIRELKN
                     IIERFVVLSAKNVIGEDEFNMLINLDMIDNETDDLSPIVVNGIMNLNDAYKIVDQIMI
                     SKAINKYGSITKAAEVIGIYPSTIHRKIKSGHIHV"
     gene            complement(4441..5478)
                     /locus_tag="KRL67_000381"
     CDS             complement(4441..5478)
                     /locus_tag="KRL67_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HBH1521287.1"
                     /translation="MNDIKDLSSYKNVWIFAEQREGKIAPVVIELLGEGRKLAKEVDA
                     ELCAILLGKDVDGLAKELITFGADKVYVADDALLEKYTTDAYTKVIKDAIDEIKPEIM
                     LFGATHIGRDLAPRIASRVGTGLTADCTKLEIDPEDKKIKQTRPAFGGNIMATIICPN
                     HRPQMSTVRPGVMDKAEKDETRTGEVIALDYKITQDDIRTTVLETVKTKKDLVSLTDA
                     NVIVSGGLGLGGPEGFEMLKKLADKLGGVVGSSRAAVDAGWIDHSHQVGQTGTTVKPN
                     LYIACGISGAIQHLAGMQSSDFIIAINKNPAAPILEIADYGVVGDLHEIVPMLIEKLD
                     SVDDLLEAIKA"
     gene            complement(5497..6282)
                     /locus_tag="KRL67_000382"
     CDS             complement(5497..6282)
                     /locus_tag="KRL67_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012703997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HBH1521288.1"
                     /translation="MKILVCVKQVPDTNEVRINKETGTLIRDGVPSILNPDDANALEE
                     ALKIKDEHDDVTITVITMGPPQADFMLRECLAMGADDAILLSDRAFGGADTWATSNTI
                     AAGISKVGDYDIIFAGRQAIDGDTAQVGPQIAEKLDIPQVTYVQDFKIEGKDIIVQRQ
                     LEDGYELIKVSTPVLLTAVKELNTPRYMSVDKIVKAYKHDVKVWTIDDLDVNKEEVGL
                     KASPTKVFRSFTPEPRGKGEILEGKADEIASKIIIGLKQKHII"
     gene            complement(6315..7448)
                     /locus_tag="KRL67_000383"
     CDS             complement(6315..7448)
                     /locus_tag="KRL67_000383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003483287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HBH1521289.1"
                     /translation="MLYNKEQELLRKAVRDFVSKELDTLPAEMDKTGVMPKELIKKLA
                     DAKFISSNIPEEYGGGGAGYVSYAIVMEEIARRCASTATFVTAGSSLASLPILYNGTE
                     EQKQKYLKGIATGELIGAFGLTEPGAGSDAGGQQTTAELVGDHYILNGRKTFITNGPF
                     CDVAIVIAVTDRSKGLRGTSAFIVESKWDGFSTGAHEDKMGIRGTETSDLIFENVKVP
                     KENLLGKEGQGFKIAMGTLEVGRIGVAALALGIAQGALDEAVKYTKQRVQFGKPIAKF
                     QNTQFTIADMETKVCAARGLVYDAAQKRDAGMRVAQESAMAKYYASEIANEVAYKALQ
                     LHGGYGFIKDYEIERMYRDARIVSIYEGTSEVQKMVISSNVLK"
     gene            complement(7496..8623)
                     /gene="hadC"
                     /locus_tag="KRL67_000384"
     CDS             complement(7496..8623)
                     /gene="hadC"
                     /locus_tag="KRL67_000384"
                     /EC_number="4.2.1.157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(R)-2-hydroxyisocaproyl-CoA dehydratase subunit
                     beta"
                     /protein_id="HBH1521290.1"
                     /translation="MEAILSKMKEVVENPNAAVKKYKSETGKKAIGCFPVYCPEEIIH
                     AAGMLPVGIWGGQTELDLAKQYFPAFACSIMQSCLEYGLKGAYDELSGVIIPGMCDTL
                     ICLGQNWKSAVPHIKYISLVHPQNRKLEAGVKYLISEYKGVKRELEEICGYEIEEAKI
                     HESIEVYNEHRKTMRDFVEVAYKHSNTIKPSIRSLVIKSGFFMRKEEHTELVKDLIAK
                     LNAMPEEVCSGKKVLLTGILADSKDILDILEDNNISVVADDLAQETRQFRTDVPAGDD
                     ALERLARQWSNIEGCSLAYDPKKKRGSLIVDEVKKKDIDGVIFCMMKFCDPEEYDYPL
                     VRKDIEDSGIPTLYVEIDQQTQNNEQARTRIQTFAEMMSLA"
     gene            complement(8623..9849)
                     /gene="hadB"
                     /locus_tag="KRL67_000385"
     CDS             complement(8623..9849)
                     /gene="hadB"
                     /locus_tag="KRL67_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(R)-2-hydroxyisocaproyl-CoA dehydratase subunit
                     HadB"
                     /protein_id="HBH1521291.1"
                     /translation="MSEKKEARVVINDLLAEQYANAFKAKEEGRPVGWSTSVFPQELA
                     EVFDLNVLYPENQAAGVAAKKGSLELCEIAESKGYSIDLCAYARTNFGLLENGGCEAL
                     DMPAPDFLLCCNNICNQVIKWYENISRELDIPLIMIDTTFNNEDEVTQSRIDYIKAQF
                     EEAIKQLEIISGKKFDPKKFEEVMKISAENGRLWKYSMSLPADSSPSPMNGFDLFTYM
                     AVIVCARGKKETTEAFKLLIEELEDNMKTGKSSFRGEEKYRIMMEGIPCWPYIGYKMK
                     TLAKFGVNMTGSVYPHAWALQYEVNDLDGMAVAYSTMFNNVNLDRMTKYRVDSLVEGK
                     CDGAFYHMNRSCKLMSLIQYEMQRRAAEETGLPYAGFDGDQADPRAFTNAQFETRIQG
                     LVEVMEERKKLNRGEI"
     gene            complement(9864..10664)
                     /gene="hadI"
                     /locus_tag="KRL67_000386"
     CDS             complement(9864..10664)
                     /gene="hadI"
                     /locus_tag="KRL67_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyisocaproyl-CoA dehydratase activator
                     HadI"
                     /protein_id="HBH1521292.1"
                     /translation="MYTMGLDIGSTASKGVILKNGEDIVASETISSGTGTTGPSRVLE
                     KLYGKTGLAREDIKKVVVTGYGRMNYSDADKQISELSCHARGVNFIIPETRTIIDIGG
                     QDAKVLKLDNNGRLLNFLMNDKCAAGTGRFLDVMAKIIEVDVSELGSISMNSQNEVSI
                     SSTCTVFAESEVISHLSENAKIEDIVAGIHTSVAKRVSSLVKRIGVQRNVVMVGGVAR
                     NSGIVRAMAREINTEIIVPDIPQLTGALGAALYAFDEAKESQKEVKNI"
     gene            complement(10703..11902)
                     /gene="hadA"
                     /locus_tag="KRL67_000387"
     CDS             complement(10703..11902)
                     /gene="hadA"
                     /locus_tag="KRL67_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isocaprenoyl-CoA:2-hydroxyisocaproate
                     CoA-transferase HadA"
                     /protein_id="HBH1521293.1"
                     /translation="MLLEGVKVVELSSFIAAPCCAKMLGDWGAEVIKIEPIEGDGIRV
                     MGGTFKSPASDDENPMFELENGNKKGVSINVKSKEGVEILHKLLSEADIFVTNVRVQA
                     LEKMGIAYDQIKDKYPGLIFSQILGYGEKGPLKDKPGFDYTAYFARGGVSQSVMEKGT
                     SPANTAAGFGDHYAGLALAAGSLAALHKKAQTGKGERVTVSLFHTAIYGMGTMITTAQ
                     YGNEMPLSRENPNSPLMTTYKCKDGRWIQLALIQYNKWLGKFCKVINREYILEDDRYN
                     NIDSMVNHVEDLVKIVGEAMLEKTLDEWSALLEEADLPFEKIQSCEDLLDDEQAWAND
                     FLFKKTYDSGNTGVLVNTPVMFRNEGIKEYTPAPKVGQHTVEVLKSLGYDEEKINNFK
                     DSKVVRY"
     gene            12580..13578
                     /locus_tag="KRL67_000388"
     CDS             12580..13578
                     /locus_tag="KRL67_000388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-2-hydroxyacid dehydrogenase"
                     /protein_id="HBH1521294.1"
                     /translation="MKILVFGARDYEEPVIKKWSEEHKDVQVDIYPENMTEENVVKAK
                     GYDGISIQQTNYIDNPYIYETLKDAGVKVIASRTAGVDMIHFDLVNENGLIVTNVPSY
                     SPNAIAELAVTQAMNLLRKTPLVKKKVCEGDYRWIAELLGTEVRSITVGVIGTGKIGA
                     TSAKLFKGLGANVIAFDQYPNSDLNDILTYKDSLEDLLKEADLITLHTPLLEGTKHMI
                     NKDTLAIMKDGAYIVNTGRGGLIKTGDLIEALESGKIRAAALDTFETEGLFLNKKMNP
                     GELTDPEINKLLSMEQVIFTHHLGFFTSTAIENIVYSSLSSAVEVIKTGTATNRVN"
     gene            13940..14467
                     /locus_tag="KRL67_000389"
     CDS             13940..14467
                     /locus_tag="KRL67_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04002 family protein"
                     /protein_id="HBH1521295.1"
                     /translation="MEKMNKVTSKKTTYIVTSALFASIICLTIAYILHIPVGGNNGYV
                     HIGDAFIYLAATILPTNYAIAASAIGAGLADLSTGAAIWVIPTIIIKPILVLFFTSKS
                     DKIINKRNIVASVVAGIVGLVLYMFAEGIIIGSFTSAFVMSLLGLLQPIGSFIVFIIL
                     GMALDKLDFKKRYFN"
     gene            14494..15090
                     /locus_tag="KRL67_000390"
     CDS             14494..15090
                     /locus_tag="KRL67_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04100 family radical SAM protein"
                     /protein_id="HBH1521296.1"
                     /translation="MNILYTIENSIYVNITNTCPCSCVFCIRNEKDEVANSGSLWLEH
                     EPSVDEVKEAFNKYNLDAYDEIVFCGYGEPLMRIDELIEVAKFIKEKSSIKIRINTNG
                     LSDLIHNKKTAILLKDVIDAVSISLNAPNKEAYNRVTQPKFGEKSFDYMLDFARYCKK
                     YIKEVAFSVVDEISPEEIEESKQLAKKLDIPLRVRHKN"
     gene            complement(15155..16738)
                     /gene="asnB"
                     /locus_tag="KRL67_000391"
     CDS             complement(15155..16738)
                     /gene="asnB"
                     /locus_tag="KRL67_000391"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HBH1521297.1"
                     /translation="MINLEEKYSIRKDTCMIKILEYNGHIRNWKVLCEELGIDSSLCR
                     DERERAILVEAYKTWGYDMANHMHGMFAFALWDTVEKKLFCLRDQFGVKPFYYYKTEN
                     GKLLYGSSIRKIMEQPGFVKELNEDMLQIYMSFTYVAGENTFFRGVKKLMPGHYLVYQ
                     NKTVFIGRYWKPEFHPDYSKSIEEWTDEIHTTLQKIMPEVKSENETAELFLSGGVDSS
                     YILAMSDIEKTGSCGYEEEWFDESKLAQQTADILGCKNSRYIITPEEYFASVPYVMYH
                     MEQPTGDASAIVFAIGCKAAVESTSICYSGEGADEFFGGYNIYHKAECYGENLKKFYI
                     GNTNIMNEGEKRKILKKYNPDVLPIKVVKRIYEETEELDTLTNMSNVDIQIWLEGDIY
                     FNIDKMSTAVGLEVRMPLTDTRIFDIASRIPSEYKVKGKENKIAFRTAASKTLPEEIA
                     FRNKLGFIVPIRIWMADDQYNKDIRDKFNSEIADKFFNIDEINEIFNDYISGNSDNWR
                     KIWMIYTFLVWYEIYFVKC"
     gene            complement(17316..18149)
                     /locus_tag="KRL67_000392"
     CDS             complement(17316..18149)
                     /locus_tag="KRL67_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HBH1521298.1"
                     /translation="MNYIIKKVLNSSVVLVHDANDNEFILLGKGIGYGKKTGEYICGS
                     NDNQMFVPVENTKSKQFLELMDDIPVDILKITQEIIVEAEKLLNSSFNKNLYLILADH
                     FNFAIERMRKEIKITNRVFWEIKNYYPNEFKVGMVAIYMVEERLGIQLPEEEAANIAF
                     HFANAIASDGNSYDTIKYAKVIGEIINIFVFSLNRTLDKKSMHYMRFITHIKFFVERF
                     FSDSMLSSGDDLLFNQMKRSNPKEMLIALKVRDFLEKKYGKKLTNEEIAFLVVHIARV
                     K"
     gene            complement(18173..19639)
                     /locus_tag="KRL67_000393"
     CDS             complement(18173..19639)
                     /locus_tag="KRL67_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000110363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HBH1521299.1"
                     /translation="MRKFPEGFLWGGATAANQFEGGWNLGGKGWSVSDVAKAHFDVDV
                     KDYKSNNEITTKDIEEGLAHPEDEVNYPKRHGSDFYHHYKEDIALMAEMGFKTYRMSI
                     AWSRIFPNGDDKEPNEEGLQFYDDVFDELISYGIEPLVTMSHYEPPLNIVLNYDGWYS
                     RQVIDMFVRYVETICERYKNKVKYWLTFNEVDSMIRHPYTTGGLVRDRFKDKNFEEVI
                     FQAMHHQFVASALATKICHEIIPNSKVGCMLTKLTYYPYTCRPEDVLAAQQKMRSIYA
                     YSDTQVFGEYPVYLLSYFKNNNIQIAKEEHDDEIMKKYPVDFISFSYYMSSCEAADTT
                     GLDITPGNTLLAVKNPYLEMSEWGWQIDSIGLRISLIELYDRYRKPLFIVENGLGAKD
                     ILTEDKKVHDQYRIDYLKEHFKCMLDAIIEDGVELWGYTSWGCIDLVSESTKQMSKRY
                     GYIYVDADDYGKGTYNRYKKDSFYWYKKVIENNSIDFK"
     gene            complement(19642..21507)
                     /locus_tag="KRL67_000394"
     CDS             complement(19642..21507)
                     /locus_tag="KRL67_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HBH1521300.1"
                     /translation="MNYKKTAIDIIEQVGGSKNILDLEHCSTRLRFKLIDRSKVNQDA
                     LKKVQGVMSIVNGAQFQVVIGNSVVDVYDEILKVCPLDINDSTIKTGEKINLGARFIE
                     FIINIFQPLIFAIAGAGILKSLLMLLAMFNILKSDSTIYTLLVAISDATFYFLPLMVA
                     VTTANVLNCNRLVAIAAVGYLLLPATTTALSEGVELFGFTIPNIAYNAQVFPAILCVS
                     FLAIMEKIFNKYSPKSIRTFFVPMMSLAITIPITLTILGPLGYNVGTIFTIIILFLYN
                     KMGFLVVGLLAVVLPFMVATGMHKALIPYAINTIGKLGYEALYMTASLAHNISESGAC
                     FAVALRTKDETLKQTALSAGISALMGITEPALYGITLQHKRAILGVVLSSAISGTFLG
                     LFTVKGFVLVGPGLASMSMFIDPNNGNNLIFAIIGFVVAILGSFIITLIIWKEDANTA
                     ESSTEDIVEEELIEEEKEVNILVSPVEGKVIDISKVNDELFASKTLGDGVAIIPANGN
                     LYAPCDSEVVMLFETKHAIGLKTKNGAEILIHIGINTVSMNGDGFKTFVKTGDNVKEG
                     DLLIKFDLDKISHANLDSTVMIVNNNGSDYAYKVLNKSYGNVKKGSILFDVKGGI"
     gene            complement(21754..22617)
                     /locus_tag="KRL67_000395"
     CDS             complement(21754..22617)
                     /locus_tag="KRL67_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HBH1521301.1"
                     /translation="MILSYFALLIRVVLLAFERVVVRLLGDEEGDIYKNIASSFLFFF
                     IGGVCLLPFSIMDRVSDWSFLIPCYISSLVYSIGSVAYVTSLATGEVSLVTPINSLNS
                     LFLLLLSVIFLGEGLSLAKVAGIIIMIGGVFILKKVSSFLKNVSVIFNNLPCRLMFLY
                     IFLQSVGRVLDKAFFVQVSPILYSTILYFFVGLNLFIFLAFKKKQKIICEIFSNKKGL
                     SILSGAINGYSYLALLIALNNLELSIAEPFSQVSMIITLILAHFIFKENIKEKIPGSI
                     LILIGGWLLLL"
     gene            complement(22672..23604)
                     /locus_tag="KRL67_000396"
     CDS             complement(22672..23604)
                     /locus_tag="KRL67_000396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HBH1521302.1"
                     /translation="MNNKKAVFFFCSDLKKDPVASNVIKCCEEIMDLNQTDIVIDDNF
                     VLEFKDKNNNLFHFVKTKDVISHNYKYYLPILNRHFRDYDFAGVVNWHEGENAPEHIL
                     TAHTIGDVPTGEFGKSNPRYFKNLINAIEDIKNENLLDEFTTLTEATHWSGTTYGEES
                     KLITEYSVPMLDIEIGSSSDSFNNSIAIQVLAKSLIRVFDCDEPLKTLLCVGGVHFEK
                     SFSDIIKNKEYNISIGHVLPNQWIVSGMYDDESGFEKLEKCINSIEGGIDCIVFHDKL
                     KGTYKEQCRKIGEKLNVPVFKHKILKNPKDLPIW"
     gene            complement(23690..24241)
                     /locus_tag="KRL67_000397"
     CDS             complement(23690..24241)
                     /locus_tag="KRL67_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBH1521303.1"
                     /translation="MQSLNEIIAENLKKIRKEKRLSLDKIAQLSGVSKSMLSQIEKCE
                     VNPTISTLKKITNGLKISFTSLMERQESDIELIQKSDIDHFIEDNGKYISYPIFPFDS
                     KRRFEIFMIEIEEGGNLDSNAELPGTQEFITVFSGEVTIKINGEDYIVSSGNSIRFKA
                     DVSHIYRNSGKGIAKMSMVVYYI"
     gene            complement(24470..24961)
                     /locus_tag="KRL67_000398"
     CDS             complement(24470..24961)
                     /locus_tag="KRL67_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH1521304.1"
                     /translation="MIDLKIENRKGKIHVKSSEIKDILELRPDFEYVQDISNTINQEN
                     ILVFDCQLTGNSFDMDDLDIEEILEELGEEIDESYFSVLFEDVRAYLKDATDEIEADL
                     QDNYLFDNLRCYFDIYNINQEFTDFKFVFLVSFKDIKISSLANLAKIVSKRQLIGASK
                     FYS"
     gene            complement(24989..25549)
                     /locus_tag="KRL67_000399"
     CDS             complement(24989..25549)
                     /locus_tag="KRL67_000399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HBH1521305.1"
                     /translation="MNKLNICIDIDGTITSPYHFLPYLNDIFNKNITEAECITHNWEE
                     LYGSGIRDVYAEFNNKYIHSYDEAKIVEGATEVISTLSKGHNLSFVTARHECLTDVTK
                     NWLSRQGFSDIDVYLLGSDYKVEKARELSCDIFIEDNPLNSVQLADDGVKVILLDTNY
                     NKDVKHQNIVRVSNWIDIEKIIKQGI"
     gene            complement(25701..26858)
                     /locus_tag="KRL67_000400"
     CDS             complement(25701..26858)
                     /locus_tag="KRL67_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HBH1521306.1"
                     /translation="MNKQSIGKLNMFFMGITQRNLDNRDFFIELEVIFKSGRKEFKGI
                     AIEEDNKYKFNFKGKSDLYTFNELLKNISKEAENYDGLVFKYVERGTIVVIEGDNKKV
                     NVKYLDNKEEVPKIDEFTASQIKNRDYYVKVGQANALLKEIGVLTKDGKIKNDKIRKY
                     NQIDHFVELIDNILKEIKDKDCITILDCACGKSYLSFVLNFYIKEVLKKNCYFIGIDY
                     SDVVIEASKNMAKNLGYKNMSFIKEDLTNYTPNRDVDLVISLHACDTATDMAIGLGIR
                     AKSEAIVVVPCCHKELLGQYRYEAMEPILKHGVFKARFADLITDGLRTLLLEGNGYDT
                     SVVEYISPLDTPKNLMIRAIKTKTNNDKALKEYKELKSQFGVEPTLEKLIY"
     gene            complement(26895..27836)
                     /locus_tag="KRL67_000401"
     CDS             complement(26895..27836)
                     /locus_tag="KRL67_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH1521307.1"
                     /translation="MRWILVFIFLYLIPLVVIFRNYKNFKRSCIYSSIYVVLATTIMI
                     SNIYMSGLNKIKESMYYHNYISDETYDEKYVSNFKEKESTDLKQDKKKNEEISNNKNL
                     NNKQDRNIQNKESNEEGSNDIKENNNSDSNVSVNNKSEKQEDNTKENNKYTSKEKNSE
                     DIKKIDMESIGIFKKEIYNIERAALVPMRECIPYTKDIAKNITKLSSIKKDVTNARNK
                     CKEVVKEYENMQIPELSKEKYTETLSNARDDVKKAYELREKSMESALKLINTKSPKYI
                     GKITEYLDLSDKQIESFKNRLNDLKEEINNTETNNIN"
     gene            complement(27923..28615)
                     /locus_tag="KRL67_000402"
     CDS             complement(27923..28615)
                     /locus_tag="KRL67_000402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HBH1521308.1"
                     /translation="MSLYAIGDLHFSTSVNKPMNIFGSNWDGHEKKIIDNWKEVVKEE
                     DMVLVLGDTSWGINLSEAKKDLDIISKLPGQKILIKGNHDYWWTTVTSLNKLYEDMRF
                     IQTNFYEYKDYAICGGRGWICPNDVKFDETDEKVYKREEHRLRLSLESARKSGHSKII
                     VITHYPPTNDKLEESLFTKLFEEYNVEKVIYGHLHGKESFKMGLKGIRNGVEYTLASC
                     DYTEFNLIKVHD"
     gene            complement(28725..29537)
                     /locus_tag="KRL67_000403"
     CDS             complement(28725..29537)
                     /locus_tag="KRL67_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase family protein"
                     /protein_id="HBH1521309.1"
                     /translation="MEYEKWNEILAPYEHAVEELKIKFKNIRKEYLAKGEYSPIEFVT
                     GRTKKISSIISKLKRINAKDIETEIDDIAGIRIMCQFVEDIYAIVDLIKVRNDMTIIG
                     EKDYITNYKDSGYRSYHVIIKYPINSIAGSKEIICEIQIRTLAMNFWATIEHSLKYKY
                     DHYIPETLAERLRRASDAAFLLDQEMSEIREDIMKAQAMYQMKSIAIRDTLNRIQELY
                     DLGDTHKAMQYQRKLDRTETDKNITEILELKQEIDILLEQYKDTKVDDKVVQ"
     gene            complement(29588..30499)
                     /locus_tag="KRL67_000404"
     CDS             complement(29588..30499)
                     /locus_tag="KRL67_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HBH1521310.1"
                     /translation="MNLTKVKGNTFFIKGGTNTGIYVFEDNTALMIDPGLCGSRPRRV
                     NKVLDENNIQVRYIINTHGHDDHYGACNQFKEYYKDVCIMSSEYDKLYIENPELFSKY
                     IVGGKSNVFMDNILKNKMLDDIKIDKSIEAGKIELNNEMFDIIEFQGHTPGSIAVLTK
                     DNVIFVGDLLIGDEILSKYDFLFLYDIQEQINSLEKLKNIDFEYLVLGHGKQVISKED
                     SYKLIEKHLNALKKYLYQIRELLVEPKSLEILLKNIINNNNLSNNYKEYHFFKSSLVS
                     VISYLVDLEEIGYILENGELLYYTKTK"
     gene            complement(30558..31289)
                     /locus_tag="KRL67_000405"
     CDS             complement(30558..31289)
                     /locus_tag="KRL67_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HBH1521311.1"
                     /translation="MTSIKNKDILNCIDYSIKNKLFNKLNDVYKSLPAGNCSGCGNCC
                     MESVGINLIEFLNIFCYLEDRVDLKKSCISKVLDYYFEEYTRKNPCPFKDTNNRCLIY
                     EVRPLNCRMFGHWKKEDYNKNLDNVIEKNNNYRELMKKQYGFEINDEVVNYRIDYCDR
                     FIPNKDYLSKSERLSFFDELMTLDSNIYSSGNIDIDFRDRGIVEYFIESLLYRNLAYN
                     VKIRISKEYKIRKRTIDRIKRIVLI"
     gene            31378..31629
                     /locus_tag="KRL67_000406"
     CDS             31378..31629
                     /locus_tag="KRL67_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH1521312.1"
                     /translation="MNRYTKIINMMGSYYTKDFEKEKKNVIKVREVKEDTVRKFFLQG
                     DCEVLVVFEDTGKEILIDDFSPEEDIKKYLGTKFINKKR"
     gene            31863..32282
                     /locus_tag="KRL67_000407"
     CDS             31863..32282
                     /locus_tag="KRL67_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH1521313.1"
                     /translation="MQIYFRVKAVGKRKPMLELTPFDVSDNINCLKEAISEIVSKNVL
                     EYNEKIPEKAIINFLTNEEIESQADIGKVGFGSIYNENKESVEKAIEVALQAFEDGIY
                     KVLINEDVIEKLDYPINLKSGDIFTFIRLTLLAGRMF"
     gene            32307..37364
                     /locus_tag="KRL67_000408"
     CDS             32307..37364
                     /locus_tag="KRL67_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4132 domain-containing protein"
                     /protein_id="HBH1521314.1"
                     /translation="MLERTRVEKNPKIIEIFKNNFTKSSSKQYYDLAKSLWDCIENSS
                     YYYREPEDKFLNMIKKYYNCHYYEIKNMDELLSGPLKNALELIFTSEQVEELCQLIKR
                     KMDYTYSGGYYRKGYRSNKLVDYFRGIIRFISSYVDLIYYDFSVIDYLTLPNKVLYYD
                     SIMSDKIALEIDKGNQKVISALEEVIFGENQTALLTRQMIIGIMKSHSDKCYEMLSKL
                     LLAAKQQEGLRQQIVESLDEGTISASIYMLKTILDNNLERFSSVIRALDTWTGLGFSD
                     QKTGIIRKCLELSYKSLTDEKFRIESINSNDNLEIYFALWATATYNIYDSYSLIENLL
                     CCQEKYKKLVALYFLSHTESTYFKHSIASKNIKGDDLEILAWVITNFYVEGSVGYYYS
                     WQNRETNFDLSNTPIDKDFNARKEQFESLKHTLEILNSKTKSFSGSVFPWTWIEISSH
                     EILKCMMTLTAYDMNAQFIDVLMENCHLMSSDLRASLIVNFVNEPKTDRQREFLLECL
                     SDKSVSNRETVLSLLRKLELTDVELDKITNLLSLKTSSIRQTVIKILLEQDDSNLENI
                     ASSLIKSSNSQKCLGGLEIVNLLKDNTDRQELCNKLILLIEDIDKSKKNTSQVQILID
                     NLLGKAKNDYCKENGFGIYNPENKVQIPEQFDFKDDSHSSSKLNKIISNISLSTCEDS
                     VSVATKKEAKKILDTDINKIIKVYEKFDELITENANYEYEVERWSGNVDKVLLGYSTY
                     NLTPIKYKVSNDTLDNYPLAELWRQTSKKLDLDYKLILDILFYSISTCGYNETEKSWF
                     SSIYSNVLDSKKQNKIYTKLEKFKFNGLTRKLLRVLLNEFDHKEIFNYCLEIYYKLRS
                     LLTIEQCKKQRVEFTKNYYRQNEYCPALNAPILNYWLDKAHSFIYDDISFTKFFYTTF
                     SNYQLLEYKFPFYTSINVFARAHELGLMTEEQVNREMISGYNASNIIRTLTNPRWAGD
                     TLEKYPWLKDNLKTVTNRIIEIESRRGDMPTEVSHLANNIQYFEGINYFVNILVALGK
                     ETFQRGYSYGSNYSKKDVLSSLLKNCYPTKEDDSKKLKSLLKNTDITELRLIESAMYA
                     PQWVNIIEKYLNWKGLKSTIWYFHAHINEGFSAQKESEVALFSPISPRSFIDGAFDVD
                     WFLDSYKTIGEERFNILYKSAKYITSGGNSHRRAQLYADATLGKLDKDMLMKEIETNR
                     NQDKLRSFSLLPMKKGDLKEATFRYEFIQKFAKESKQFGAQRRESESKACTIALENLA
                     RSAGFGDVNRMMWALETEKLNEVAHLFKPKDIDGTSIWIDVDENGKASTKVQKGNKSL
                     KSIPKAIAKHPYVLELKSVQKDLKSQYSRAKQSLEQAMKNEIVFTVKEIANIYKNPVI
                     CPLIKNLVWVCDDNIGYVYNDKSKFFICNIEGETYKLTAKNKLKLAHPVHLFESGKWS
                     EFQHDLFSKQIVQPFKQVFREYYRINSDELSEKNISRRYAGHQIQPKKTVALLRNQLW
                     TVDYEDGLQKVFYKENIVAKMFALADWFSPADIEPPTIETVEFFDRKTYKSLELTSIP
                     PILFSEVMRDIDLVVSVAHAGGVDPETSHSTVEMRIAIAKELLLLLKINNVNFEGSHA
                     LITGNLGEYSVHMGSGIVHKKGMGALNILPVHSQSRGKIFLPFADEDPKTAEIISKIL
                     LLAEDNKIKDPSILSNL"
     gene            complement(37548..38285)
                     /locus_tag="KRL67_000409"
     CDS             complement(37548..38285)
                     /locus_tag="KRL67_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBH1521315.1"
                     /translation="MIKVLICDDDKIVRDDIANIVKESGYVDYVKKVKDGIEAIEFIK
                     NEKIDLLIIDIDMPYKNGIDSAKEILSIDKDIHIVFVTGFADYSLESFKVHPIDFVVK
                     PFKKEKILDSINIAIDHINSHKIANNNFIEDTLFVYKIRKQIHMINFDDIVMFEKNSR
                     AINLHTKDEDIIKFYENFDDLKKRLPPNFFMTHRQYIVNLRFIHKVIPINKSSLEIRF
                     INFQENAILNKSLEKDFLYRFYRVKRF"
     gene            complement(38303..39238)
                     /locus_tag="KRL67_000410"
     CDS             complement(38303..39238)
                     /locus_tag="KRL67_000410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HBH1521316.1"
                     /translation="MDNNFVFPIVILTLFIFSNLIMNKYKFYKELDILKRGILTFFIS
                     CFALILFMEGTKYIQLRLGYSFLLEGVLNIILFLVLIILSFYTGFKVIRLINNNNEQE
                     YKLQKLDSKINHKDNIIIKLRSQKHDYLKHLQVVYSLLNTGLDEDAKEYIMNISNSFK
                     SKNSRYGKVSYLDAIASLKYEECLEKDISFDVYIQDFMDNLEIEPNDMSSVLLNIIDN
                     AIESLSTIKKEKKYIRLHAYEDEFQYIIDIKNNGPKIHNVESIFWDGFSTKEGQDRGY
                     GLHLVKNILDKYNYSILVNSDDFLTEFVILIPKYY"
     gene            39446..42031
                     /locus_tag="KRL67_000411"
     CDS             39446..42031
                     /locus_tag="KRL67_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBH1521317.1"
                     /translation="MKDNLKIVLRYIKSYKARSLAIALSIILATSLIVGIGTLSRSAQ
                     QASVDRFRRELGPDHVYFKDIKRNQLELIKSSKDIKNLGITSHYGYTDINEKLPINIE
                     YVNKNYLTSHSKLIKGHFPKANNEVVVEKWVLNSMGLKPEINQDITFKLFKKEKPETF
                     KVVGILEDRAIQKKQGMCEIFLNINESKLDNFSYVYVEFNEGSDINTNINNIVKNTML
                     DKNSVGKNKTLIEATMENGTLDGSSKYIAIAMSIFSGIVIYSIYVISIYQRVQEYGIL
                     RAIGATNFRIFKLMFYELFILALIAIPTGICTGMVGAQIFNKTAGNIQFEGDVRVTPF
                     VIPDKVIFLAIGCIILTILIISFFTYLKIRRISPIDAIRKTFGTDKNISKVNSLISKL
                     TSNMSATKSISTKNILRNKKGFIIIILSMSIGGIMVIKENYKYSFSDTQNKDSQERAY
                     MNADFILSNFTFKLNENYKANSNADINGLNDNQIDKIKNIDGIDKVKTASILNTRIEL
                     DKINKLDYYNIISSSPYYNEFPILTKNKTTGKYTLAQRLRGYNDEMINSLEKYLVSGS
                     INLEKMKKENLAIVYVPQISKTNKYSKSYIPGVGTSVVDIKVGDTIKVKYPKGEIDTD
                     LYMRQKDNYEYLEYEFKVGAIVSYPFADNNSYSGDEGIDVITSSEYLKKLTGVNKYNT
                     VYIDLKENANIKKINTLLGKIGSEVPGTTTVDMSVEKENSDNMTARAMIYAYGIVAVM
                     FIISVFNIINNISYNLTSRTSEFGMLRAIGISDRRFKNMILYEGILYGTLSSVITIVA
                     GLIIQFRMYYTYNFVSYGLGFSIDYKIYIIVILANIIVGILATYIPLRKINKISIVES
                     INITE"
     gene            42043..42714
                     /locus_tag="KRL67_000412"
     CDS             42043..42714
                     /locus_tag="KRL67_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBH1521318.1"
                     /translation="MPILETINLGKIYGKKETSVHALKDANLKINKGEFVAIIGPSGS
                     GKSTFLHLVGGLERPSNGTIKVDGKDICCLSDKELARYRRQKVGFVFQQYNLIPVLNV
                     KENIELPLKLDNKKIDKEYIDDLINLLGLNERKNHLPNQLSGGQQQRVAIARALSAKP
                     SIILADEPTGNLDSKTTEEVMDLLKSSIKKYNQTLIIITHNENIARKADRIISIIDGE
                     LKLTL"
     gene            42741..43082
                     /locus_tag="KRL67_000413"
     CDS             42741..43082
                     /locus_tag="KRL67_000413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH1521319.1"
                     /translation="MLLILGNLNLNLDFDYRIIREENDDVDIFIDINYRSLDIDTDGS
                     NLFNSRIQFPFVRALILRLNKNNQCMTIHLLRDIDLFSAFANFEVDYTDSIINIKNQN
                     EKVILNKFIKK"
     gene            complement(43442..46084)
                     /gene="adhE"
                     /locus_tag="KRL67_000414"
                     /gene_synonym="adhC"
     CDS             complement(43442..46084)
                     /gene="adhE"
                     /locus_tag="KRL67_000414"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HBH1521320.1"
                     /translation="MEKKEKVVEKSNVEVCSPEIVNSVETLRMRLEEIRLAQKEFATF
                     TQEQVDKIFLAASTAANQQRIPLAKMAVEETGMGIVEDKVIKNHFASEYIYNAYKDTK
                     TCGVIEKDEAFGFTRIAEPVGVLSAVIPTTNPTSTAIFKSLIALKTRNGIIFSPHPRA
                     KNCTIEAARVVHDAAVKAGAPKGLIGWVDVPSIELTNVVMAEADLILATGGPGMVKSA
                     YSSGKPAVGVGPGNVPAIIDESADIKMAVSSILVSKSFDNGMICASEQAVIVPEKIYE
                     EVKKEFKYRGAHFLNKEETEKVAKVVIIDGSLNARIVGQPAHVIAKMADVEVPKTARI
                     IIGEVESVELNEPFAHEKLSPVLAMYKSKSFEDAVAKAEKLVADGGYGHTSSLYADSI
                     NHPERVEKFVNAMKTCRVLVNTPSSQGGIGDLYNFKLAPSLTLGCGSWGGNSVSENVG
                     VKHLLNIKTVAERRENMLWFRAPEKVYFKKGCLGVAAREFKDVMDKKKAFIVTDSFLY
                     NNGYTKKLTDLLDEMGIKHTTFFDVAPDPTLACAKEGAKAMADFQPDLIIAVGGGSAM
                     DAGKIMWVMYEHPEVDFQDLAMRFMDIRKRVYVFPKMGEKAYFAAIPTSAGTGSEVTP
                     FAVITDQDSGVKYPLADYELMPNMAIIDADMMMEMPPRLTASSGVDALTHALEAYVSM
                     LRTEPADGLALQAGKIIFEYLPRAYKNGKNDKEAREKMAMASTMAGMSFANAFLGICH
                     SLAHKLGAFHHVQHGVANALLINEVIKFNCAEAPNKMGAFSQYRYPDCIQRYAEFASF
                     AGIKGNTDQEKVDNLIKAIDELKAKVGLPKTIKEAGVEESKFLEGLDAMVEQAFDDQC
                     TGANPRYPLMSELKEIYLKVYYGK"
BASE COUNT        15497 a         7720 c         5681 g        17441 t
ORIGIN      
        1 ttatgaactt cttacacttt catgtaagca cagactatat cttatccttc ggaattatcc
       61 gctaaggtct acccacttcc actaccaata gcttgtagtg tacttccctc aagaggaata
      121 gtcgttgaag tttctcctat tcggagctta cctgctgatt gcccatttta aagtacttag
      181 gatttaacct tataccatct aaccaatttt ttctactttc gtaactttca cacttaggtt
      241 tatttcatcc ttatgtttta gtttggttag ctttaggggt ttccagcaat tcaagtagta
      301 ttggataggt gttatcctat ctctacatat aagtttccct atatgctgac taatttatct
      361 actaactcat gactaaagtc acaagtgtgc gtagctattt tttaatcaaa tatatatatt
      421 ctctatatat aacttcttct ccattaaaca atatctcatc attttcagtg tctacttgag
      481 ttcctggatt tgaaacttca cttctattta ctactacaga cccttgttta caatacttct
      541 ttatttcact tctacttcca taacctaaat ttgaaagaat tttatctata cgttgctttt
      601 tagccattaa aaatgctctc cattctcata atatttttct ttttcaaata ctgtaaatgc
      661 taaatccata ctttctacat ctaaattatt tcttatacta tcagttatta ttcttgctac
      721 tatatcttgt acttcttgcc ctctatcaaa ccaagctact tcaacaaaag gatatacatc
      781 tgctatttta ccatctctta ttgcaactga ctttatacat tctatttcaa agtagtctct
      841 tggacatttt acagcttcaa ctaaatcatc tatcattttt tcacttattt tacatatatc
      901 attttcatta attcctctta tttttatctg tggcatagtt tacctccttt attttaaatt
      961 tttagtgaat tatcatccac ttaattccct tcgataacgt ttgaagtggt agcttcttgg
     1021 tcaagacaac tattgttatc agtatctcca aactaataag gtagttccta cccttatagt
     1081 tagcattata tagctaagtt tcctaaattg tttatattct ttattatttt atttaatcct
     1141 attgtcttct atttaacatt atacttgtaa tttatagtta ttacaattta ctatatactt
     1201 tttttgattt aacgttataa tataagtgat gggtaaattt taaaagttaa taggaggagt
     1261 ttaaaatggc attaattact acaaaagaaa tgtttaaaaa agcttacgaa ggtggatttg
     1321 ctataggtgc atttaacata agtgacttag aacaattaca aggtgtttta aaagctgcta
     1381 aagctaaaaa ttcttatgtt atgatacaag cttctatgtc agctgttaaa tatgctggac
     1441 ctcatacatt agttgaaatg gttaaggctg cttctgatga aataggtgta gatgtagctc
     1501 ttcacttaga ccatggtcca aatatggatg ctattaaaac ttgtattgat gctggtttct
     1561 cttcagttat gatagatggt tctcattttg actttgaaga aaatgttaga ataactaaag
     1621 aagctgttga atatgcacac tctaaaggtg ttgttgttga agcagaactt ggtgttctag
     1681 ctggaacaga agatgatgtt acttctgatg ttcacaagta tactcaacca gctgaggcag
     1741 ttgagttcgt tgaaagaaca ggtgttgatt cattagcaat agctattgga acttctcatg
     1801 gtgctttcaa attcaaagga gaagctaaat taagattcga tatactagaa gaaatccaaa
     1861 gcaaattacc aggtttccca atagttcttc atggtgcatc tgctgttgac caaaacgctg
     1921 ttgctacatg taatgaattt ggtggaaata tagctggtgc taaaggtgta cctgtagata
     1981 tgttaagaaa ggcatcttct atggctgttt gtaaaataaa catggatact gacttaagac
     2041 tagctatgac tgctgctatc agaaaattct tagctgaaaa tccaaaagaa tttgacccaa
     2101 gaaaatacat tggtgctggt agagatgcta tacaagctgt agttgaaagc aagatagatg
     2161 atgttttagg ttctgctaac tctataaact aatataactt ttaaatattt ataaaagaaa
     2221 agagatgact taaagtaatt aaatttattt ttaagccatc tcttttaagt tgtatcaatt
     2281 atgtatctta taatatttta attatattgt ctttttaaac ttacagctaa acgtgaatat
     2341 gtccactctt tatctttcta tggatggtag aaggatatat acctataact tcagcagcct
     2401 ttgttattga accatactta tttatagcct tgcttatcat tatttggtcc actattttat
     2461 atgcatcatt aagattcata attccattta ccactattgg tgataaatca tctgtttcat
     2521 tatcaatcat atctaaattt atcagcatgt taaattcatc ttctccaatt acatttttag
     2581 ctgataatac tacaaatctt tcaatgatgt tttttaattc tcttatgttt cctggccaag
     2641 aatattcttc taataactca ataactttag gtgaaacttc tacttctcta ttgtactttt
     2701 cattgtaaag ttttaagaaa taatttacta atggtactat atcctctttt ctttctctaa
     2761 gaggaggtat ttttactgga ataacactta atctatagta caaatcttgt ctaaatttaa
     2821 ggttatttct aaggtcttca tgagaaatat tagtagcact tatatatcta acatctattt
     2881 ttataggctt actaccacct attctagtaa tagtattttc ttgtataacc ctaagtaatt
     2941 ttttttgcat ctgcattggt aactcaccta tctcatctaa gaaaatagtt ccaccatttg
     3001 cctcttcaaa aagtcccttt ttaccttttt tcttagctcc agtaaaagaa ccttcttcat
     3061 atccaaaaaa ctctgactct aataattcat ttggtatagc tccacagttt atagctataa
     3121 aaggtttgtc tctccttgaa ctatacttat atattgttct tgctattatt tcttttccta
     3181 cacctgattc tccatttata aatatagaag aatctgaggc tgcagctctc ttaacaactt
     3241 tttttatatt gtccataatc ttactttgac ttatatactc tgtgtcttct gatatatcac
     3301 tatattttat ctgagattca agtttaataa cccttgaaat atctcttcct gttagtactc
     3361 ctcctgtaag ttttccctta gaatcccatc ttggaactgc tgtatatgca ataagcttcc
     3421 catctgggta aacgatattt ctataaatct ttttcttcac tttaagcatg tttaatatta
     3481 ttggttcttc aactaatcct tgtctcacaa ggtctgttat atttctacca agtacctcag
     3541 ttcttttaaa aggtaatatc tcatcactga cagtattcat cttttcaatt accccatctt
     3601 tgttaaacac aacaacaact tcatctatat gctctattat atcttcaatt actctatcag
     3661 atattagaga cttctcaaat aaataaacta tattattttc attattatta tctttcatat
     3721 tgtatatatg tgcagtcatc cttaactctc tgaaagctat tttcttttta ctatctgtca
     3781 gtatttctgt tttgtcaaaa tttgaatcta attcatttat atttctaggc ttttttatat
     3841 ctagaaaact aaaaatacta tctattagat gattacagta tactatttcg cctgattctt
     3901 tacataaaat ggctggattt tctacatgtt ctataagccc tattaaatta ttatccatta
     3961 atattcgcac ctttcattaa gtaatttatt gaactacata caaaatttat tgtttgctta
     4021 caataaatac catcagcaca tataatcaac tccttataat attcgtcttt tcaagtagtt
     4081 tatttcatta aaacgtaact aagatataat tttaatgatt atagtttgtt aaaaatttca
     4141 ttttgctttt aattgtattt tatcacatat ttagtaatta tttgtctatt taatacaatt
     4201 taattaaatt taagatttat ttaaaaaaac aaagataatc cttacagaat ataactcaca
     4261 atttgtaagc aatatacata taaggattat ccagattatc ttgtattata aaattttatt
     4321 caaacagact ttttaataac tgacaaatac acactagcaa taacttatca aagggttaat
     4381 aattatgtta ctaactaata agacttattc aatcagtaat atttatatag tttgtatcta
     4441 ttaagctttt atagcttcta ataaatcatc aacactatct aatttttcta taagcattgg
     4501 aactatttca tgtaagtctc caactactcc atagtcagcg atttctaaaa ttggagctgc
     4561 tgggtttttg tttatagcaa ttatgaaatc tgatgattgc atacctgcta aatgttgtat
     4621 tgctcctgat ataccacaag ctatatatag gtttggttta acagttgttc ctgtttgacc
     4681 tacttggtga gaatggtcta tccatccagc atcaacagcc gcacgagaag aaccaactac
     4741 tccgcctaat ttgtcagcta atttcttaag catttcaaat ccttctggtc cacctaatcc
     4801 taaaccacct gatactataa catttgcatc tgtaagagat actaaatctt tcttagtttt
     4861 aactgtttct aaaacagtag ttcttatatc atcttgagtt attttgtagt ctaatgctat
     4921 aacttcacca gttcttgttt cgtctttttc agctttatcc ataacaccag gtctaactgt
     4981 agacatttga ggtctatggt ttggacaaat gattgtagcc attatgtttc caccaaatgc
     5041 tggacgagtt tgttttattt tcttatcttc tgggtctatt tctaacttag tacagtcagc
     5101 tgttaatcca gttccaactc ttgaagctat tctaggtgct aagtctctac ctatatgagt
     5161 tgctccgaaa agcattattt ctggttttat ttcatctatt gcatctttta ttacttttgt
     5221 atatgcatca gttgtatatt tttctaaaag agcatcatct gcaacataaa ctttatcagc
     5281 tccaaaagtg attaattctt tagctaatcc atcaacatct tttcctaata atattgcaca
     5341 aagttctgca tctacttctt tagctaattt tcttccttct cctaataatt ctataactac
     5401 tggagctatt tttccttctc tttgttctgc aaatatccat acgtttttat aagaacttaa
     5461 atcttttata tcattcatag tttgtactcc tccaacttat ataatgtgct tttgttttag
     5521 accgattatt attttactag ctatttcatc agctttacct tctaagattt ctccttttcc
     5581 tcttggttca ggagtaaatg atctaaatac tttagttggt gatgctttta aaccaacttc
     5641 ttctttattt acatctaaat catcaattgt ccaaactttt acatcatgtt tatatgcttt
     5701 tactatttta tcaacagaca tgtatctagg tgtatttaac tcttttactg ctgttaaaag
     5761 tacaggtgta cttactttta ttaattcgta tccatcttct aattgtcttt gaactattat
     5821 atcttttcct tctattttga aatcttgtac atatgtaact tgaggaatgt ctaatttctc
     5881 tgcaatttgt ggtccaactt gtgcagtatc tccatctatt gcttgtcttc ctgcaaatat
     5941 tatgtcatag tcacctactt tagatattcc cgctgcaata gtatttgatg tagcccaagt
     6001 atcagctcct ccaaatgctc tatcacttaa taatattgca tcatcagcac ccatagctaa
     6061 acactctctt agcataaagt ctgcttgagg aggtcccatt gttataactg ttatagttac
     6121 atcatcatgc tcatctttaa tttttaaagc ttcctctaat gcatttgcat catctgggtt
     6181 taatatactt ggaactccat ctcttataag agtacctgtt tctttattta ttcttacttc
     6241 gttagtatct ggaacttgtt ttacgcaaac taatattttc aaagtctata cctccaattt
     6301 cattagatta tttattattt taatacgttt gatgaaatta ccattttttg aacttctgat
     6361 gttccttcgt atattgatac gattctagca tctctgtaca ttctttcgat ttcataatct
     6421 tttataaatc catatccacc gtgaagttgt aatgctttat aagcaacttc atttgcaatt
     6481 tctgatgcat agtatttagc catagcagat tcttgagcaa ctctcattcc tgcatctctc
     6541 ttttgtgctg catcataaac taatcctcta gctgcacaaa cttttgtttc catatcagct
     6601 atagtaaatt gagtattttg gaatttagct ataggcttac caaattgaac tctttgtttt
     6661 gtatatttaa cagcttcatc taaagcacct tgagctattc ctagagctaa ggcagcaaca
     6721 cctattctac caacttctag agtacccatt gctatcttaa atccttgacc ttcttttcca
     6781 agtaagtttt cttttggaac tttaacgttt tcaaatatta agtcagaagt ttcagttcct
     6841 cttataccca tcttatcttc atgagctcct gttgagaaac catcccattt actttctact
     6901 ataaacgctg atgttcctct aagtccttta cttctatctg taactgctat tactatagct
     6961 acatcacaga atggtccatt agttatgaaa gtttttctac catttaatat atagtggtct
     7021 ccaactaatt ctgctgttgt ttgttgtcca cctgcatcag aaccagctcc tggctcagtt
     7081 aaaccaaatg ctcctattaa ttcacctgtt gctatacctt ttaagtattt ttgtttttgc
     7141 tcttcagtac cattgtaaag tattggtaat gaagcaagag aagaaccagc tgtaacaaaa
     7201 gtagctgttg aagcacatct tctagctatt tcttccatta ctatagcgta agaaacatat
     7261 cctgctcccc caccaccata ttcttctggg atattactgc ttataaattt tgcatcagct
     7321 aattttttga ttaattcctt aggcattaca cctgttttat ccatttctgc tggtaaagta
     7381 tctaattctt tactaacaaa atctcttact gcttttctta aaagttcttg ttctttgtta
     7441 tataacatag cttcctccaa ttttcatatt ttatagtaaa tttattttaa tgaacttacg
     7501 ctaaactcat catctcagca aaagtttgaa tacgagttct ggcttgttca ttattctgag
     7561 tttgttggtc gatttcaaca tataaagtag gtattccact atcttctata tcttttctaa
     7621 ctaaaggata atcgtattct tctgggtcac agaatttcat catacagaag ataacaccat
     7681 ctatatcttt ctttttaact tcatctacta taagtgaccc acgttttttc ttagggtcat
     7741 aagctaatga acatccttct atgtttgacc attgtcttgc taatctctct aacgcatcat
     7801 cacctgctgg tacatctgtt ctgaattgtc ttgtttcttg tgctaagtcg tcagctacaa
     7861 ctgatatatt gttgtcttct aaaatgtcta atatatcttt agaatcagct aatatacctg
     7921 ttaataaaac tttctttcca gaacagactt cttctggcat agcatttaat tttgctatta
     7981 aatctttcac tagctcagta tgttcttctt ttctcataaa gaatccactc ttaattacta
     8041 agcttcttat tgatggtttt atagtattag aatgtttata agctacttca acaaagtctc
     8101 tcatagtttt tctatgttca ttgtaaactt ctatactttc atgaattttt gcttcttcta
     8161 tttcatatcc acaaatttct tcaagttctc tttttacgcc tttgtactca ctgattaagt
     8221 attttacacc agcttcaagt tttctatttt gtgggtgtac taatgatata tattttatat
     8281 gaggtactgc tgatttccag ttttgtccta aacaaattag tgtatcacac atccctggta
     8341 taataactcc agataattca tcataagcac cttttaatcc atattctaaa catgattgca
     8401 ttattgaaca tgcaaatgca gggaaatatt gtttagctaa atctaattct gtttgtcctc
     8461 cccatatacc tactggaagc attccagctg catgtataat ttcttctggg caataaactg
     8521 ggaaacaacc tatagctttt ttaccagttt cacttttata tttttttaca gccgcatttg
     8581 gattttcaac tacttctttc attttagata aaatagcttc cattatatct cacctctatt
     8641 aagttttttt ctttcttcca ttacttcaac taaaccttga attcttgttt caaattgagc
     8701 attagtgaaa gctctagggt ctgcttggtc accatcaaat ccagcatatg gtaatccagt
     8761 ttcttcagct gctcttcttt gcatttcata ttgtattaaa ctcataagtt tacagcttct
     8821 gttcatatga tagaatgctc catcacattt accctctact aaagaatcaa ctctatattt
     8881 tgtcatacgg tctaggttta cattgttaaa catagtacta tatgctactg ccattccatc
     8941 taaatcatta acttcatatt gtaatgccca agcatgtggg taaacactac ctgtcatgtt
     9001 aactccaaat ttagctaatg ttttcatctt gtatcctata tatggccaac aaggtatacc
     9061 ttccatcatt attctgtatt tttcttcccc tctgaaagaa gatttaccag ttttcatgtt
     9121 gtcttctaat tcttctataa gtaacttaaa tgcttctgta gtttcttttt tacctctagc
     9181 acaaactatt acagccatgt aagtaaataa gtcaaatcca ttcattggag aaggagaaga
     9241 atctgctggt aaactcatag aatacttcca tagtcttccg ttttcagctg atattttcat
     9301 tacttcttca aacttcttag ggtcaaattt ctttcctgat ataatttcta gttgttttat
     9361 agcttcttca aattgagctt taatataatc tattcttgat tgagtaactt cgtcttcatt
     9421 attgaaagtt gtatcaatca ttattaaagg tatatctaat tctcttgaaa tattttcata
     9481 ccattttata acttggttac atatattgtt acagcaaagt aggaaatctg gagctggcat
     9541 atccaaagct tcacatccac cattttctaa aagaccaaaa tttgttcttg catatgcaca
     9601 taggtcaata gaatatcctt tagattcagc tatttcacat aattctaaag aacctttttt
     9661 agctgctact ccagctgctt ggttttctgg atataatacg tttaagtcaa atacttctgc
     9721 taactcttga ggaaatactg atgttgacca acctacaggt cttccttctt ctttagcttt
     9781 aaatgcattt gcatattgtt cagctaataa atcattaatt actactctag cttctttttt
     9841 ttcagacatt ttcatccctc tttttatata tttttcactt ctttttgtga ttcttttgct
     9901 tcatcaaaag catataacgc tgctcctaaa gcaccagtta attgaggtat atcaggtaca
     9961 ataatttctg tgttgatttc tcttgccata gctcttacaa taccactatt tctagcaact
    10021 ccaccaacca taactacatt tctttgtact cctattcttt ttactaggct agaaactctc
    10081 tttgctactg aagtatgaat acctgccact atatcttcaa tttttgcatt ttcagataaa
    10141 tgtgatataa cctcagactc tgcaaatact gtacatgtac tgcttattga tacttcattt
    10201 tgagaattca tagatatact tccgagttca gatacatcaa cctctattat ttttgccatt
    10261 acatctaaaa atcttcctgt acctgcagca catttgtcat tcataagaaa gtttaatagt
    10321 cttccattat tatctaattt taataccttt gcatcttgac cacctatgtc aataatggtt
    10381 cttgtctctg gaattatgaa atttacccct ctagcatgac agcttaattc acttatttgc
    10441 ttatcagcat ctgaatagtt cattcttcca tatcctgtaa ctacaacttt tttaatatct
    10501 tctcttgcaa gacctgtctt gccatataat ttttctaaaa ctcttgatgg tccagtagtc
    10561 ccagtaccag aggatattgt ttcagaagct acaatatctt ccccattctt taagattact
    10621 ccctttgatg cagttgaacc tatatctaat cccattgtgt acatctttag cattctcctt
    10681 ttaaaaaatc tagaattact aattaatatc ttacaacttt actatcttta aagttattta
    10741 ttttctcttc atcatagcct aaagatttta atacttctac agtatgttga cctacttttg
    10801 gtgctggtgt atattcttta attccttcat ttctaaacat aactggagta ttaactaaga
    10861 cacctgtatt tccgctatcg tatgttttct taaataagaa gtcatttgcc caagcttgtt
    10921 cgtcatctaa taaatcttca cagctttgaa ttttttcaaa tggtaagtct gcttcttcta
    10981 ataaagctga ccactcgtct aatgtttttt ctaacatagc ttctccaact atcttaacta
    11041 aatcttcaac atgattaacc attgaatcta tgttattata tctatcgtct tctaatatat
    11101 attctctatt tataacctta cagaatttgc ctaaccactt gttgtattgt attaaagcta
    11161 attgaatcca tcttccatct ttacatttat atgtagtcat taatgggctg tttggatttt
    11221 ctcttgataa aggcatttca tttccgtatt gtgctgttgt tatcattgtt cccattccat
    11281 atatagctgt atggaaaaga cttactgtta ctctctcacc tttaccagtt tgagcttttt
    11341 tatgtaatgc tgctaaactt cctgctgcta gtgctagacc tgcatagtgg tcaccaaatc
    11401 ctgctgctgt atttgctgga gatgttcctt tttccataac agattggcta actcctcctc
    11461 ttgcgaagta tgcagtatag tcaaatcctg gtttatcttt taaaggacct ttttcaccat
    11521 atcctaatat ttgagagaat attaatcctg gatacttatc ttttatttgg tcataagcta
    11581 tacccatttt ttctaatgct tgaactctaa cattagttac aaatatgtct gcttctgata
    11641 ataatttatg taatatttct actccttctt ttgattttac attaatactt acaccctttt
    11701 tatttccatt ttctaattca aacatagggt tttcatcatc tgatgctgga gatttaaatg
    11761 ttccacccat aactcttatt ccatcacctt ctataggttc aatcttaata acctctgcac
    11821 cccagtcacc taacattttt gcacaacatg gtgctgcaat gaaacttgaa agttctacta
    11881 ctttaactcc ttctaaaagc ataacgtttt cctccttaat agtatcaatt ataaaatttc
    11941 gattaaatca ctcataaaat acttttttct gatattaact gtttgatgtc aaagttccta
    12001 atcttatcta aaataactta aaaattatta aaattatttt ttattgtttt attgtcttac
    12061 acctatattg ttaagcattt atcgtgccaa ctttatattt aatcaaaata tcgcttgatt
    12121 tgaatgtttt ttaacaatat acatttgaat attctttttc atatcgtggc atcttgcaac
    12181 tatccattgc attttgctat tctttctatg cccataaaag ttttttttat atattttttt
    12241 atgaaaaagc ttgacatttc aaaaaaagtt ttcattgcta aactcaaaaa cacatgttat
    12301 cattgtaatt aatcagcaag ttagttaact aatttcaaac ttagataatt ttttaataat
    12361 atttaatcta aaaaataaca atttctttga taaattttta gagtaattat tacattaaat
    12421 aaaaaattac taaatatcaa aataacacag ggaaaacaat ttcaaagcta aatttttaac
    12481 cttaaaaatt ttgtataaaa aatataacct tatttgatac atattatttc taaatgaaaa
    12541 attaccataa taatttaata attacaggag ggtattgata tgaaaatact agtatttgga
    12601 gcacgcgatt atgaagaacc agtaataaaa aaatggtctg aagaacataa ggatgttcaa
    12661 gtggatattt atcctgaaaa catgactgaa gaaaatgtag ttaaagctaa agggtatgat
    12721 ggtatatcta tacaacaaac taactatata gataatcctt atatttatga aactttaaaa
    12781 gatgctgggg ttaaagttat agcttcaaga actgcagggg ttgacatgat acattttgat
    12841 ttagttaatg aaaatggact tatcgttaca aacgttcctt cttattcacc taatgcaata
    12901 gctgaattag ctgttactca agctatgaac cttttaagaa agactcctct agtaaagaaa
    12961 aaagtctgtg aaggtgatta ccgttggata gctgaacttc ttggaacaga agttagatct
    13021 attacagttg gtgttatagg tacaggaaaa ataggtgcta cttctgcaaa gttattcaaa
    13081 ggcctaggag ctaatgtaat tgcatttgac caatatccaa atagtgattt aaacgatata
    13141 ttaacttaca aagattcttt agaagacctt ctaaaagaag ctgaccttat aacattacat
    13201 actcctttac ttgaaggaac aaaacatatg ataaataaag atactctagc tataatgaag
    13261 gatggagctt acatagtaaa tactggccgt ggtggtttaa ttaagacagg ggacttaata
    13321 gaagcactag agtcaggaaa aattagagct gctgcccttg atacatttga aactgaagga
    13381 ttgttcttaa ataaaaaaat gaatccagga gaattaactg acccagaaat aaataaactt
    13441 ctttctatgg aacaagttat attcactcat caccttggtt tcttcactag tacagcgatt
    13501 gaaaatatag tttattctag tttaagtagt gctgtagaag ttataaaaac aggaactgct
    13561 actaatagag taaattagta ttttataaat atattttttc attaattagc tattatctaa
    13621 caaaatagcc tttaatttcc caaatataag agagtcagct caatatatat tgagtagact
    13681 ctcttatatt attttttata ttaaacttgt acccctctta ttcatagcaa attaaattta
    13741 tattaactta tcctaactac tcctacttta tatattttac ctatttttac ttttctaatt
    13801 atatacattt gaatgggtac aatctgtgtt ttacaaaaaa aacacctaac aaactatgaa
    13861 aattgtatga ttaatattaa tatatttata agaaaacata acaattatgt tagctctgga
    13921 tttttaggga gggagtatat tggaaaaaat gaataaagtt acttctaaaa aaactactta
    13981 tattgttact tcagcattat ttgcatccat aatatgcctg acaatagcat atatattaca
    14041 tattccagtt ggtggaaaca atggatatgt tcatatagga gatgctttta tttatctagc
    14101 tgcaactatt ttgccaacaa actatgctat agctgcttct gcaattggtg ctggtctagc
    14161 tgatttatct acaggtgctg ctatctgggt aataccaact ataatcataa aaccaatact
    14221 tgtattattt ttcacttcta aaagtgacaa gataataaat aagagaaata tagttgcttc
    14281 ggttgtagca ggtatagtag gattagtttt atacatgttt gctgaaggta taattattgg
    14341 aagttttaca agtgcatttg taatgagctt acttggctta ttacagccaa taggaagttt
    14401 tattgttttt ataatcttag gtatggcact tgataaactt gattttaaaa aacgttattt
    14461 taattaagaa tttaaattta tggagggaaa attatgaata tcttatatac aattgaaaat
    14521 agtatctatg taaatataac taatacatgt ccatgtagtt gtgttttttg tataagaaac
    14581 gaaaaagatg aagttgcaaa tagtggtagt ttatggttgg agcatgaacc aagtgtagat
    14641 gaagtaaaag aagccttcaa taaatacaac ttagacgcct atgatgaaat cgtattttgt
    14701 ggatatggcg aaccattaat gagaatagat gaactgatag aggtagcaaa gtttataaaa
    14761 gaaaaaagta gcataaagat aagaataaat acaaatggtc taagtgattt aatacataac
    14821 aaaaaaacag ctattctgtt aaaagatgtt atagatgcag tatcaataag tctcaatgcc
    14881 ccaaataaag aagcttacaa tagagttact caaccaaagt ttggagaaaa atcatttgat
    14941 tatatgttag attttgcaag atattgcaaa aaatatataa aagaagtagc tttttcagta
    15001 gtagatgaaa tatctccaga agaaatagaa gaatctaagc aactggcaaa aaaacttgat
    15061 ataccattga gagttagaca taagaattag caagtaaaat tataaaacat taagaatcac
    15121 ctgaatggtc tttcaggtga ttcttcctct tgtattaaca ttttacaaaa tagatttcat
    15181 accacactag gaatgtgtaa atcatccaaa tctttctcca attatcagag tttccactaa
    15241 tgtagtcatt aaaaatctca ttaatctcat ctatgttaaa gaatttatct gctatctcac
    15301 tattgaattt atctcggata tccttattat attggtcatc agccatccat atacgaattg
    15361 gcacaataaa tcccaattta tttctaaaag caatctcttc tggtaatgtt tttgatgcag
    15421 ctgtacgaaa ggcaatttta ttttcttttc ctttcacttt atattccgat ggtattctgg
    15481 atgcaatatc aaatattctt gtatctgtaa gaggcattct gacttccaat ccaactgcag
    15541 tactcatttt atctatatta aaataaatat ctccttccaa ccatatttga atatctacat
    15601 ttgacatgtt agtaagtgta tctagttctt ctgtttcttc ataaattctt tttaccactt
    15661 taataggaag cacatcagga ttgtatttct taagaatctt tcttttctca ccctcattca
    15721 taatgttcgt atttccaata taaaatttct tcaaattctc tccatagcac tctgccttat
    15781 ggtatatatt atatccacca aagaactcat ctgctccttc tccagagtaa cagatactcg
    15841 tgctctcaac tgctgccttg catccaatcg caaaaacgat agcagatgca tctccagttg
    15901 gttgctccat atggtacatt acataaggta ctgacgcaaa atactcttca ggtgtaatta
    15961 tgtaccttga atttttacac ccaagaatat cagctgtctg ttgtgctaat tttgattcat
    16021 caaaccactc ttcttcataa ccacatgaac ctgttttttc tatatctgac attgccaata
    16081 tataagatga atcaacacct cctgatagga ataactctgc agtttcattc tctgatttta
    16141 cctctggcat aattttctga agtgttgtat gaatttcatc tgtccattcc tcaatggact
    16201 tgctgtagtc aggatgaaat tcaggtttcc aataacgccc aataaatact gttttattct
    16261 gatagacaag gtaatgtcct ggcataagct tctttactcc acggaagaat gtattttcac
    16321 ctgctacata tgtaaaagac atataaatct gtaacatgtc ttcatttaac tctttcacaa
    16381 atcctggttg ctccataatc ttacgaatag atgaaccata gagaagcttc ccattttcag
    16441 tcttataata atagaaaggc tttactccaa actggtctct taggcagaaa agtttctttt
    16501 ccacagtatc ccataatgca aaggcaaaca ttccatgcat gtgatttgcc atatcatatc
    16561 cccaggtctt gtatgcttct actaaaattg ctcgttcacg ctcatctcta cataatgaag
    16621 aatcgattcc aagctcctca cagagaactt tccagtttct aatatgtcca ttgtattcta
    16681 ggatttttat catacatgtg tcttttcgta ttgaatactt ttcttctaaa tttatcatat
    16741 gtgtatcctt tcatattaaa acatttaact cttaaattta ttgattgttt ttatttatat
    16801 ttatttactt ataatcattg aaaattaact tttttcaaag taaacccttt tttaattagt
    16861 actaatatgc tctttattaa ggtcaccaat atgattatca gtgttataac attgaatttt
    16921 ttattcttca taaatattgt cctcctttat attatcagct aaatacaata tatcttatat
    16981 tacttacata ttctttaaga aattctgaag attttataaa attttctgta gtataattaa
    17041 ttaaaaatac aatgctagtt ataaagcata ttttctatta aacaaaaaat agttaaaata
    17101 aattcataac cacataaact tattttaact atttgcatat tttaattata aatattaact
    17161 taaattttta acaggataca ctcataaaat aaacataata aacaacaaaa taatatgtct
    17221 cagtttcttt atatacttta ccttctttca attttctacc atgtagacgt ataagataat
    17281 attagtaatt cactctattg ctgtaattta ctctattact taactcgtgc gatatggaca
    17341 accaagaaag ctatttcctc atttgttaac ttttttccat atttcttttc tagaaaatct
    17401 ctaactttta atgcaattaa catctctttt ggatttgacc ttttcatttg attaaataat
    17461 aaatcatcac cactgcttaa catactatct gaaaaaaatc tttccacaaa aaattttata
    17521 tgtgttataa atctcatata atgcatactc tttttatcta aggttctatt caaagaaaaa
    17581 acaaaaatat taattatttc tccaattact ttggcatact taatcgtatc atatgaattt
    17641 ccatctgaag ctatagcgtt tgcaaaatgg aaagcaatgt tggctgcttc ttcctctggt
    17701 aattgaatac ccaatctctc ttctaccata taaattgcaa ccattccaac cttaaactca
    17761 tttggataat aattttttat ttcccaaaat actcgatttg taatttttat ttctttacgc
    17821 atcctttcaa ttgcaaagtt aaaatggtct gctaatatta agtataagtt tttattaaaa
    17881 gatgagttca ataacttttc tgcctctact attatttcct gtgttatctt taaaatgtcg
    17941 acaggaatat catccattaa ttctaaaaat tgttttgatt ttgtattttc aacagggaca
    18001 aacatttgat tatcattgga accacaaata tattctcctg tttttttacc atatccaatt
    18061 cctttcccta gcaaaataaa ttcattatca tttgcatcat gaactaaaac aacgctcgag
    18121 ttcaaaactt tctttattat gtagttcatt ttatttgctc accttctatt ggtcatttaa
    18181 aatctatact attattttca attacttttt tataccaata aaaactatct ttcttatatc
    18241 gattatatgt cccctttcca taatcatcag catccacata aatatatcca taacgttttg
    18301 acatttgttt cgttgattca cttaccaaat caatacaacc ccatgaagtg tatccccaaa
    18361 gttcaacacc atcttcaata atcgcatcta acatacattt aaagtgttct tttaaataat
    18421 caattctata ttggtcatga acttttttat cttcagttaa aatatcttta gctcctaatc
    18481 cattttcaac tataaataaa ggctttctat atctatcata taattcaatc aaagatatac
    18541 gtagacctat tgaatcaatt tgccaacccc attcactcat ttctaaataa ggatttttta
    18601 cagctaataa cgtattccca ggtgtaatat ctaatccagt tgtatcagct gcctcacaag
    18661 aagacatata gtaagaaaat gaaataaaat ctactggata ctttttcata atttcatcat
    18721 catgttcttc ttttgctatt tgaatgttat tatttttaaa atatgacagt aaatatactg
    18781 gatactcacc aaaaacctgt gtatcactat atgcataaat acttctcatt ttttgttgag
    18841 ctgccaaaac atcttctggt ctacatgtat atggataata tgttaatttt gttaacatac
    18901 accctacttt tgaatttgga ataatctcat ggcaaatttt tgttgcaagg gcactagcta
    18961 caaattgatg atgcatagct tgaaaaatga cttcctcaaa gtttttatcc ttaaaacggt
    19021 ctcgaaccaa accaccagta gtgtatggat gacgaatcat agaatcaact tcattaaatg
    19081 taagccaata tttcactttg tttttatatc tttcacagat agtttctaca tacctaacaa
    19141 acatatctat aacttgtcgt gaataccatc catcataatt taaaacaata tttaatggtg
    19201 gttcataatg tgacatagta acaagtggtt caattccata acttattaac tcatcaaata
    19261 catcatcata aaactgtaaa ccttcttcat ttggttcttt atcatctcca tttggaaaaa
    19321 tacgagacca agcaatactc attcgatatg tcttaaatcc catttctgcc attagagcaa
    19381 tatcttcttt atagtgatga taaaaatcac taccatgacg ttttggataa tttacttcat
    19441 cttcaggatg tgctaatcct tcttctatat cttttgttgt gatttcatta ttagatttat
    19501 agtctttcac atctacatca aagtgtgcct ttgctacatc tgatacagac caccctttac
    19561 cacctaaatt ccaaccacct tcaaattgat tagcagctgt agctcctccc caaagaaatc
    19621 cttctggaaa ctttctcata tttatattcc tccctttaca tcaaacaaga tacttccttt
    19681 ttttacattg ccataagact tgtttaacac tttataagca taatctgacc cattattatt
    19741 tacaatcata acagttgaat ctaagttagc atgtgagatt ttgtctaaat caaacttaat
    19801 taacaaatct ccttctttta cattatcgcc tgtcttaaca aatgttttaa atccatctcc
    19861 attcatactt acagtattta ttccaatatg aatcaaaatc tcagccccat tttttgtttt
    19921 taaaccaatt gcatgttttg tttcaaatag catcacaact tctgaatcac aaggtgcata
    19981 taaatttcca ttggctggta taattgcaac tccatctccc aaagtcttac ttgcaaataa
    20041 ttcatcatta accttactta tatctattac ttttccttct actggagata ctaagatatt
    20101 tacttctttt tcttcttcta ttaattcttc ttcaacaata tcttcagttg atgactctgc
    20161 tgtatttgca tcttccttcc agataatcag tgtaataata aatgaaccta atattgcaac
    20221 aacaaaacct ataattgcaa aaattaaatt gtttccatta tttgggtcaa taaacatgct
    20281 catactggca agccctggtc caacaagtac aaatcctttt actgtaaata atcctaaaaa
    20341 tgtaccagaa atcgcacttg acaatacaac acctagtatt gctcttttat gttgtaaagt
    20401 aataccatat aaagctggct ctgtaattcc catcaaggca gaaattcctg cagataaggc
    20461 tgtttgtttt aatgtttcat cttttgtacg taaggctact gcaaaacaag caccactttc
    20521 agaaatattg tgagctaatg aagctgtcat atataatgct tcataaccca actttccaat
    20581 tgtattaatt gcataaggta ttaatgcttt gtgcattcct gttgcaacca taaacggaag
    20641 aacaacagca agtaatccta caactaagaa tcccattttg ttatataaga ataaaataat
    20701 gattgtaaaa attgtaccaa cgttatatcc taaaggacct aaaatagtta gagtaattgg
    20761 aatagtaatt gccaaagaca tcataggaac aaagaaagtt cgaattgatt ttggtgaata
    20821 cttgttaaaa attttctcca ttattgccaa gaaagacaca cataagatag ctgggaaaac
    20881 ctgtgcatta taggcaatgt ttggaattgt aaatccaaat agttcgacac cctcacttaa
    20941 agcagttgtt gttgctggaa gcaataagta tcctacagcg gcaatagcta ctaaacgatt
    21001 acagttcaat acattggcag tagttacagc taccataaga ggtaggaaat agaatgttgc
    21061 atctgaaata gcaactaata atgtatatat agtagaatct gattttaaaa tattaaacat
    21121 tgctaataac atcaacagtg attttaaaat accagctcct gcaatagcaa aaatcaatgg
    21181 ttgaaaaata ttaataataa attctataaa tcttgctcct aagtttattt tttctccagt
    21241 ttttatagta ctatcattta tatctaaagg acaaactttt aaaatttcat cataaacatc
    21301 aacaacacta tttccaatga caacttgaaa ttgtgcccca ttaactatac tcataacacc
    21361 ttgtactttt tttaaagcat cctgattgac tttacttcta tcaatcaact taaagcgtag
    21421 tctagttgaa cagtgttcta aatctaaaat attttttgaa ccaccaactt gttcaataat
    21481 atcaatggca gtttttttat aattcatctt gtaatctcct tctccataat tttttatgaa
    21541 tggccattca agatgcaaaa aaggcagaac ctatataaac accttgaaaa atcaaagcaa
    21601 tatattcgat cctgcctgca ttaccagtaa catgtcttct atttatacta ttttgtatta
    21661 ttatattaac atttgcctaa aagaattgtc aatacttttt taaattttct gatactaact
    21721 aatagcacct ttataattca ttttttatat caattaaagt aacagtaacc acccaccaat
    21781 tagtatcaaa attgaacctg gtattttttc ttttatattt tctttgaata taaaatgagc
    21841 aagtattaaa gttattatca tagatacttg agaaaatggc tctgctatac taagctctag
    21901 attatttaaa gcaattaata atgctaaata agaatatcca ttaatagcac cacttaaaat
    21961 tgaaagcccc tttttattac taaaaatctc acaaattatt ttttgtttct ttttaaatgc
    22021 taaaaagata aataaattta aacctacgaa aaaatacagt atagttgaat ataatatagg
    22081 agatacttgt acaaaaaaag ccttgtccaa tactcttcct actgactgca aaaatatgta
    22141 taaaaacatt aatctacatg gtaaattatt aaaaattaca gagacattct ttaaaaaaga
    22201 gcttactttt tttaatataa acactccacc aatcataatt atgattcctg caacttttgc
    22261 taaacttaaa ccttcaccta aaaatataac tgataaaagc agtaaaaaca atgaatttaa
    22321 actatttatt ggagtaacaa gtgaaacttc cccagttgct aaggacgtta catacgctac
    22381 tgaaccaatt gaatagacta aactacttat ataacatggt attaaaaacg accaatcact
    22441 aactctatcc attatactaa atggaagtaa acatactcca cctataaaga aaaatagaaa
    22501 tgatgaagct atatttttat aaatatctcc ttcttcatca cctaataatc ttacaactac
    22561 tctttcaaaa gctaatagca ctactctaat taataatgca aaataagata atatcatttt
    22621 tgacctcgct taaaaattaa tccgacttat tgttatatta actgtataag actaccatat
    22681 aggtaagtct tttggatttt ttaatatttt atgtttaaat acaggtacat taagtttttc
    22741 acctatttta cgacattgtt ctttataggt accttttaat ttatcatgaa aaactataca
    22801 atcaattcca ccttcaatac tatttataca cttttctagt ttctcaaatc ccgattcatc
    22861 atcatacatt ccacttacaa tccattgatt tggcaataca tgtccaattg atatgttata
    22921 ttctttattt tttataatat ctgagaaaga tttctcaaaa tgtacccctc caacacataa
    22981 taaagtcttc aaaggttcat cacaatcaaa tactctaatt aatgattttg ccaatacctg
    23041 tattgctata ctattattaa aactatcgct actacttcct atctcaatat ccaacatagg
    23101 tacagaatat tctgtaatta atttagattc ttcaccatat gttgttccag accagtgagt
    23161 tgcttctgtt aatgtagtaa attcatcaag tagattttca ttttttatat cctcaatagc
    23221 attgattaga tttttaaagt atcttggatt tgactttcca aattctcctg ttggaacatc
    23281 tccaattgta tgagctgtta ggatatgttc tggagcattt tctccctcat gccaatttac
    23341 aactcctgca aaatcataat ctctgaaatg tctatttaaa attggaagat aatatttata
    23401 attatgactt attacatctt ttgtttttac aaaatgaaat aagttattgt ttttatcctt
    23461 aaactctaat acaaaattat catcaataac aatatcagtt tgatttaaat ccattatttc
    23521 ttcacaacac tttatcacat tactggctac tgggtctttc tttaaatcac tacaaaagaa
    23581 aaaaactgct tttttattat tcatccataa atcacactcc tataacttaa atataatata
    23641 tcttcatacc taaaatcatc taaatatcac tctagttcca ttaaattatt tatatataat
    23701 atactaccat actcatttta gctatgcctt tgcctgagtt tctatatata tgtgatacat
    23761 cagctttaaa tcttatagaa tttccactag atacaatgta atcttctcca tttattttaa
    23821 tggttacttc gccactaaaa acagttataa actcttgtgt tcctggaagt tcagcatttg
    23881 aatctaaatt tccaccttct tctatttcaa tcataaatat ctcaaatctt cttttggagt
    23941 caaatggaaa tattggataa cttatatact ttccattatc ctcaataaaa tggtctatat
    24001 cacttttttg aatcaattct atgtcacttt cctgcctctc cattaatgat gtaaatgaga
    24061 tttttagtcc attagttatt ttctttaaag tagatatagt aggatttacc tcacactttt
    24121 caatttgaga tagcatactc ttacttacac cactaagttg tgcaatcttg tctaaactaa
    24181 gacgtttttc tttccttatc ttttttaaat tttcagcaat tatttcattt aaactttgca
    24241 taaatacccc ctaatacata ctaatataag ataaattctt ttgattatta aataaactta
    24301 gtaatttaat agaaactttt tatccattat aatgtacatt atgtttaata tacattactt
    24361 tttttagaat gtaaatagtt ttctgaaatt ttgtgaaaat gaacaaataa aaaactagta
    24421 acctatattt ttacaaggct actagctttt attataataa tatttcattt taactataaa
    24481 acttagatgc acctataagc tgtctcttag aaacaatctt agctaggttt gctaatgaag
    24541 aaatttttat atctttaaaa gaaactaaaa atacaaactt aaaatctgta aattcttgat
    24601 ttatattgta tatatcaaaa taacatctca aattatcaaa taagtaatta tcttgtaagt
    24661 ctgcttctat ctcatcagta gcatctttta aatacgctct tacatcttca aataaaacac
    24721 tgaaataaga ctcatctatt tcttcaccta actcttccaa tatttcttct atatctaaat
    24781 catccatatc aaaagagttt cctgtcaact ggcaatcaaa taccaatata ttttcttgat
    24841 ttatagtatt tgatatatct tgtacatatt cgaagtctgg acgtaattct aaaatgtctt
    24901 ttatttcact gctctttaca tggatttttc cttttctatt ttcaattttt aaatctatca
    24961 tatgctcccc ctttcaatct acaaatcttt aaataccttg ttttataatc ttttcaatat
    25021 ctatccaatt actaactcta actatatttt gatgtttaac atctttatta tagttagtat
    25081 ccagaagtat tactttaaca ccatcatcag ctagttgcac tgagtttaaa gggttatctt
    25141 ctataaatat gtcacaactc agttctcttg ccttttcaac tttataatca cttcctaata
    25201 agtaaacatc tatatctgaa aatccttgtc tcgatagcca atttttagtt acatcagtta
    25261 aacactcatg tctagccgtt acaaaagata aattatgtcc tttagataat gtgctaatta
    25321 cctctgtagc accctctaca atctttgctt catcatatga gtgtatatac ttattattaa
    25381 actccgcata cacatctctt attcccgaac cgtataattc ttcccaatta tgtgttatac
    25441 actctgcttc agttatattt ttattaaaga tatcatttag gtatggaaga aaatgataag
    25501 ggcttgttat tgttccatct atatcaatac atatattcaa tttgttcatt tttacctcca
    25561 tgtaacattt attgatttga ttaatcaaat attatagcac agctttaatt ataaatcaaa
    25621 actctagata tgactatacc tatataatac caaaaagctt gcatatttaa gcaagctttt
    25681 cgtaaatttt acatttttaa ctaatatata agtttttcta aggttggttc cactccaaat
    25741 tgtgatttca gttctttata ttcttttaat gctttgtcat tattcgtttt agtttttatc
    25801 gctcttatca ttaaattttt tggtgtatca agtggagata tgtattctac tacagaagta
    25861 tcatatccat tgccctccaa taacaatgtt cttaaaccat cagttattaa atctgcaaat
    25921 ctagctttaa atacaccatg ttttaatatt ggttccatag cttcatatct atattgccct
    25981 aataactcct tatgacaaca tggtactact actatagctt cagattttgc tctaattcct
    26041 agacctattg ccatatctgt agctgtatca caagcatgta gacttattac aaggtcaaca
    26101 tccctattag gtgtataatt agtcaaatcc tcttttataa atgacatatt tttataaccc
    26161 aagtttttag ccatattttt tgatgcttct ataactacat ctgaataatc tatgcctata
    26221 aaataacagt ttttctttaa tacttctttt atataaaaat ttaagacaaa gcttaagtaa
    26281 gattttccac aagcacaatc caagatagtt atacagtctt tatcctttat ttcttttaaa
    26341 atattatcta tcaattcaac aaaatggtct atctgattgt attttcttat tttatcattt
    26401 tttatcttac catcttttgt aagtactcca atttctttta gaagtgcatt tgcttgtcct
    26461 acttttacat aataatctct gttttttatt tgagatgccg taaattcatc tattttaggt
    26521 acttcttcct tattatctaa atattttaca tttaccttct tattgtctcc ttcaattaca
    26581 acaatagttc ctcgctcaac atatttaaat actaatccat cataattttc tgcctcttta
    26641 gaaatattct taagcaattc attaaacgta tataaatctg attttccttt aaaattaaat
    26701 ttatatttat tatcttcctc tattgcaatt cctttaaact cttttcttcc agatttaaaa
    26761 attacctcta actcaataaa aaaatcccta ttatcaaggt ttctttgagt aatccccata
    26821 aaaaacatat ttagttttcc tatactctgt ttattcatca taatctccct tttaacttat
    26881 atccttaaat cttattaatt gatattattt gtttctgtat tgtttatttc ttcttttaaa
    26941 tcgttcaatc tatttttaaa actttctatc tgcttatctg ataaatcaag atattctgtt
    27001 atcttcccta tatacttagg gcttttagta tttattaatt tcaaagcact ttccatagat
    27061 ttttctctaa gctcatatgc tttctttaca tcatctctag cattactcaa tgtttctgta
    27121 tacttctcct tagaaagttc tggaatctgc atattttcat attcttttac aacttcttta
    27181 cacttgtttc ttgcatttgt aacatctttt tttatactcg ataatttagt tatatttttt
    27241 gctatatcct tagtgtaagg tatacattct ctcattggaa ctaaagctgc tctttctata
    27301 ttataaattt ctttcttaaa aattcctata ctttccatat ctattttttt tatatcttca
    27361 ctattttttt ctttacttgt atatttgtta ttttcttttg tattatcttc ttgtttttct
    27421 gatttattat tcactgaaac attactatca gaattattat tttcttttat atcattactt
    27481 ccttcttcat tactttcttt attttgtata tttctatctt gtttgttgtt taaattttta
    27541 ttatttgata tttcttcatt ctttttctta tcctgtttta aatcagtact ttctttttcc
    27601 ttaaaatttg atacatactt ttcatcatat gtctcatcag agatataatt gtgataatac
    27661 atagactctt ttattttatt aagtccactc atataaatat tacttatcat tattgttgta
    27721 gctaatacca cataaatact actgtagata caagacctct taaaattctt gtaatttctg
    27781 aatatcacta ctaaaggaat aagatataaa aatataaaaa ctaatatcca cctcataagt
    27841 cgtgtctcct ttctcaaaac cgaaatccct caaatattta tttttaatca tgtatcttat
    27901 agtatttacc aatttatgtt ttttaatcgt gaacttttat taaattaaat tctgtatagt
    27961 cacaagatgc taatgtatac tctactccat ttcgaatccc cttcaatccc attttaaaag
    28021 attctttacc atgcaagtga ccataaatca ccttttcaac attatattct tcaaataact
    28081 ttgtaaataa agattcttct aatttatcat tcgtaggagg ataatgagta atcacaatta
    28141 tttttgagtg accactttta cgagcagatt ctaatgataa cctaagtcta tgttcttctc
    28201 ttttataaac tttttcatca gtctcatcaa atttaacatc attaggacat atccagcccc
    28261 tacctccaca aattgcataa tctttatatt cataaaaatt tgtctgaata aaacgcatgt
    28321 cttcatatag cttatttaaa ctcgtaacgg ttgtccacca gtaatcatga tttcctttta
    28381 taagtatttt ctgacctgga agtttgctta ttatatctaa atctttttta gcttccgata
    28441 aattaattcc ccaagatgta tctcctagta ctaagaccat atcttcttcc ttaacaactt
    28501 ctttccaatt atctataatt ttcttttcat gaccatccca attacttcca aagatattca
    28561 ttggcttatt gactgatgtt gaaaaatgta aatcacctat agcatataaa ctcataatat
    28621 cattcctttc ttgattacac taaataggct gtctcttttt agaaattatt ttccattaag
    28681 aggcagcccc attaatattt agcaaatttt ctacaagtag ttttttactg aactacttta
    28741 tcatctacct tagtatcctt atattgctct agtaatatat ctatttcttg ttttaattcc
    28801 aaaatttcag ttatattttt atctgtttca gttctatcaa gttttctttg atactgcata
    28861 gctttatgtg tatcacccaa gtcatataac tcttgtattc tatttagagt atctctaata
    28921 gctatagact tcatttggta catagcctga gctttcatta tgtcctctct tatttcactc
    28981 atttcttggt ctaaaagaaa ggcagcatca gatgctcttc taagtctttc agcaagagtc
    29041 tcaggtatgt aatggtcata tttatatttt aatgaatgtt ctattgtagc ccagaaattc
    29101 attgcaagag ttcttatttg tatttcacat ataatttcct ttgaacccgc aatggagttt
    29161 atagggtatt ttattataac atgatagctt ctataaccac tatctttgta atttgtaata
    29221 taatcttttt ctcctattat agtcatatca tttcttactt ttatcaaatc aactatagca
    29281 tatatatcct caacaaactg gcacattatt cttataccag caatatcatc aatttcagtt
    29341 tctatatctt ttgcatttat cctttttaac ttagatatta tggaagatat tttttttgtt
    29401 ctgcctgtaa caaactctat gggagaatat tcaccttttg ctaaatattc ttttcttata
    29461 tttttaaatt ttattttcaa ttcctctact gcatgttcat aaggtgctag tatctcattc
    29521 catttttcgt attccatatt gctcaccctt tatttgtttt ttatgacctt ctttatttta
    29581 tcataattta ttttgttttt gtatagtaaa gcaattctcc attttccaat atatacccta
    29641 tttcttctaa atcaactaag taacttatga cagatactaa cgaagattta aaaaaatgat
    29701 actctttata attattgctt aaattattgt tatttataat gttttttaat aaaatttcta
    29761 aagatttcgg ttctacaagc aactctctta tttgatataa atattttttt aaagcattca
    29821 aatgtttttc gattaattta taagaatctt cttttgatat aacttgcttg ccatgcccta
    29881 aaactaagta ctcaaaatct atatttttta atttttctaa tgaatttatt tgttcttgta
    29941 tatcatatag aaacaagaaa tcgtattttg atagaatttc atctcctatc aataaatcac
    30001 caacaaaaat cacattatcc ttcgttaaaa cagctatact accaggtgta tgaccctgga
    30061 actcaattat atcaaacatt tcattgttta attctatttt tccagcttct atagatttat
    30121 ctatttttat atcatccaac attttatttt ttaagatatt atccataaat acattgctct
    30181 ttcccccaac aatatacttt gaaaacaatt ctggattttc tatatataat ttatcgtatt
    30241 cagatgacat tatacacaca tctttataat actctttaaa ttgattacaa gctccatagt
    30301 ggtcatcatg accatgagta tttataatat atctaacctg tatattattt tcatctaata
    30361 ctttatttac tctcctaggt cttgaaccac ataaccctgg gtctatcata agtgctgtgt
    30421 tatcttcaaa tacatatatt cctgtattcg tgccaccttt tataaaaaag gtatttcctt
    30481 ttacttttgt aagattcacc ttcttcacct cattttgaca atatttatga tacaatttat
    30541 tacttactaa tatgaaacta tataagaaca atccttttta ttctatctat agttcttttt
    30601 cttattttgt attctttaga tatccttatt ttcacattat atgctaaatt tctataaagt
    30661 aatgactcta taaaatattc aactatacct ctatccctaa agtctatatc tatatttcca
    30721 cttgaataaa tatttgagtc taatgtcata agttcgtcaa aaaaacttaa tctctcagat
    30781 ttgcttaaat aatctttatt tggaataaat ctatcacaat aatctatcct gtaatttact
    30841 acttcatcat ttatttcaaa tccatattgc tttttcatta actctctata gttattattt
    30901 ttctcaataa cattatctaa gtttttatta taatcctctt ttttccaatg tccaaacatc
    30961 ctacaattta atggtctaac ttcatatatt aaacatctat tattagtatc tttaaatgga
    31021 catgggttct ttcttgtata ctcctcaaaa taataatcta atactttact tatacagctt
    31081 ttttttaaat caactctatc ttcaagatag caaaatatat tcaaaaactc aattaaattt
    31141 atacctactg actccataca acaatttcca catccagaac aatttcctgc tggcaaggac
    31201 ttatatacat catttagttt gttaaagagc ttatttttaa tggaataatc tatacaattt
    31261 aaaatatctt tattttttat actagtcatt attattactc cttgttataa ttattaagtt
    31321 aatacaaaca attattataa catatttttt aaatttaatc gcatatggag gtattttatg
    31381 aatagatata caaaaataat taatatgatg ggaagttatt acacaaaaga ctttgaaaaa
    31441 gaaaaaaaga atgtaataaa agttagagaa gttaaagaag atacagttag aaaatttttc
    31501 ttacaaggtg actgtgaagt tcttgtagta tttgaagaca cagggaaaga aatacttatt
    31561 gatgactttt ctcctgaaga agatatcaaa aaatatttag gaacaaaatt cataaataaa
    31621 aaaagatagt tatcaaaaat aattctaaaa ttaaaattta ataaatgtac agttataatt
    31681 ctatataatt atagttaaaa gttatctttt attgcttttt tagactttaa gatagctttt
    31741 tttctatttt ttgtaaaata attccataat tcacattaat aattcacaaa atgatataat
    31801 ataacaaata gataaatttt taaatttcta aatacataat taatattttg cgaggtgatt
    31861 ttatgcaaat ttattttaga gttaaggctg ttggcaaacg caaacctatg ctagaactta
    31921 ccccttttga tgtatctgac aacatcaatt gcctaaaaga agctatctct gaaattgtat
    31981 ctaaaaatgt tcttgaatat aatgagaaaa tcccagaaaa agcaataata aacttcttaa
    32041 ctaatgaaga aattgaatct caagctgata ttggaaaagt tggatttggt tctatttaca
    32101 atgagaataa agaatctgta gaaaaggcaa tagaggtagc gttacaggca tttgaagatg
    32161 gaatctacaa agtgcttata aatgaagatg ttattgaaaa actcgattac cctataaatc
    32221 tgaaaagtgg agatatattt acttttataa gacttacctt gcttgctgga agaatgtttt
    32281 aaaataaata atttggaggt acataaatgt tagagcgtac tagagtagaa aaaaatccta
    32341 aaataattga aatttttaaa aataatttta ctaaatcttc ttcaaaacag tactacgact
    32401 tagcaaaatc tctttgggac tgcatagaaa atagttcata ctattataga gaacctgaag
    32461 acaaattctt aaatatgata aagaaatact acaattgcca ctactacgaa attaaaaata
    32521 tggacgagct cctttctgga cctttaaaaa atgcattaga attaatattt acttctgaac
    32581 aagtagaaga actttgccaa ttaattaaaa gaaagatgga ttatacatat tcaggaggat
    32641 attatcgtaa agggtatcgc tcaaataaac ttgtggatta ttttagaggt attataaggt
    32701 ttatttcaag ctatgttgat ttaatttatt atgatttttc agttatagat tatcttactc
    32761 ttccaaataa ggtcttgtac tatgactcta taatgagtga taaaatagca ctagaaatag
    32821 acaaaggaaa tcaaaaagta ataagtgcct tagaagaagt aatatttgga gaaaatcaaa
    32881 ctgctctact tacaagacaa atgataattg gtatcatgaa gagccatagt gataaatgct
    32941 atgaaatgct ttctaaactt cttttggcag ccaaacaaca agaaggactt agacagcaaa
    33001 ttgtagagag tttagatgag ggtacaattt ctgcatcaat atacatgtta aaaactattt
    33061 tggacaacaa tttagagcga tttagttctg tcataagagc cttggataca tggacaggac
    33121 ttggtttttc agaccaaaaa actggaataa tacgtaaatg tttagaactt tcatataaat
    33181 cattgactga tgaaaagttc cgtatagaat caattaatag caatgataat cttgaaattt
    33241 attttgcctt atgggctact gcaacttaca acatatatga ctcttactct cttattgaaa
    33301 acttactttg ttgccaggaa aaatataaaa aacttgtagc tctttacttt ttatcgcata
    33361 cagaaagtac atattttaaa cattctatag catctaaaaa cattaagggt gatgatttag
    33421 agatactagc atgggtaatt acaaatttct atgtagaagg ttctgttggc tactactata
    33481 gttggcaaaa cagagaaact aattttgact tgtcaaacac tcctattgac aaagacttta
    33541 atgctagaaa agaacaattt gaaagtttaa agcatacttt agaaatttta aattcaaaga
    33601 caaaatcatt ttcaggaagt gtatttccat ggacttggat tgaaatttct tctcatgaaa
    33661 tacttaaatg tatgatgact ttaacagcat acgatatgaa tgctcaattt atagatgtgc
    33721 ttatggaaaa ttgtcatctt atgagttcag atttaagagc ttcacttata gtcaattttg
    33781 taaatgaacc aaagacagat agacaacgtg aatttttatt ggaatgttta agtgataaga
    33841 gtgtttccaa tagagaaacg gtattgagtc tacttagaaa gcttgaacta acagatgtag
    33901 aattagataa gataaccaat ctattgtctt taaagacaag ctctattaga caaacagtaa
    33961 taaaaatttt acttgaacaa gatgattcaa atttggaaaa tattgcttct tcactcataa
    34021 aatcttcaaa ttctcaaaaa tgccttggag gtcttgaaat tgtaaactta ttaaaagaca
    34081 atactgatag acaagaacta tgtaataagc ttattttact tatagaagat attgataaat
    34141 ctaaaaagaa tacatctcaa gttcaaatac taattgataa tttgttaggt aaagcaaaaa
    34201 atgattactg caaagaaaat ggttttggaa tatataaccc tgaaaacaaa gttcaaattc
    34261 cagaacaatt tgattttaaa gatgattctc atagctcatc aaagcttaat aaaattattt
    34321 caaacataag tttatctact tgcgaggatt ctgtttctgt agcaacaaaa aaagaagcaa
    34381 aaaaaattct cgatacggat ataaataaaa taataaaagt ttatgaaaaa tttgatgagc
    34441 ttattactga aaatgcaaat tatgagtatg aagttgaaag atggtcaggt aatgtagaca
    34501 aagtactttt aggttattct acttataatt tgactcctat taaatataaa gtttctaatg
    34561 atactcttga taattatcca ttggcagaac tttggagaca aactagcaaa aaacttgact
    34621 tagattacaa attaatactt gatatattat tttatagtat aagtacatgt ggatataatg
    34681 aaacagaaaa aagttggttt tcatctatat attcaaatgt ccttgactct aaaaaacaaa
    34741 ataaaatata tacaaaactt gaaaaattta aatttaatgg acttacaaga aaacttttaa
    34801 gagttttact aaatgagttt gaccataaag aaatatttaa ctattgtttg gaaatatact
    34861 ataaacttag aagcttatta actatagaac aatgtaaaaa acaaagagtt gaatttacta
    34921 aaaactatta cagacaaaat gaatactgcc ctgctttaaa tgcccctatt ttaaactatt
    34981 ggcttgataa agcacattca tttatttatg atgatatttc ttttactaag tttttctaca
    35041 ctacgttttc aaattatcag ttgctagaat ataagtttcc tttctatact agcataaatg
    35101 tgtttgctcg tgcacatgaa ctaggattaa tgactgaaga gcaagttaat agagaaatga
    35161 taagtggata caacgcttca aatataataa gaacattaac aaatcctaga tgggctggtg
    35221 acacattaga aaagtaccct tggcttaagg ataatttaaa aactgttaca aaccgaatta
    35281 tagaaattga atcacgtaga ggtgatatgc caacagaagt gtctcatcta gctaataata
    35341 tacaatactt tgaaggtata aattattttg taaatatttt ggtcgctctt ggtaaagaaa
    35401 ctttccagag aggatattca tatggtagca attattcaaa aaaagatgtt ttaagttctc
    35461 tacttaaaaa ttgctatcct actaaagaag atgattcaaa aaaattaaaa agtttactta
    35521 aaaataccga cattactgag ttaagattga tagaatctgc tatgtatgca cctcaatggg
    35581 taaacataat tgaaaaatat ttaaactgga agggtctgaa aagtacaatt tggtacttcc
    35641 acgcacatat aaatgaagga ttctctgcac aaaaagaatc agaagttgct ctattctccc
    35701 ctatttcacc acgtagtttc attgatggtg catttgatgt agattggttt ttagattcat
    35761 ataaaactat tggagaagaa agattcaata tactttacaa atcagctaaa tatataacta
    35821 gtggtggtaa ctctcataga cgtgcacaac tctatgctga tgctacactt ggtaagcttg
    35881 ataaagacat gttgatgaaa gaaattgaaa caaatagaaa tcaagataaa ttgagaagtt
    35941 tctctttact tcctatgaaa aaaggagacc taaaagaagc aaccttccgt tatgaattta
    36001 ttcaaaagtt tgctaaagaa agcaagcaat ttggtgcaca acgtcgtgaa agtgaatcaa
    36061 aagcttgtac tatagcgcta gaaaatctag ctagaagtgc tggatttgga gatgtaaatc
    36121 gtatgatgtg ggctcttgag actgaaaaac taaatgaagt tgctcatcta tttaaaccaa
    36181 aagatattga tggtacttca atttggatag atgttgatga aaacggaaaa gcgtctacta
    36241 aagttcaaaa aggcaataag tctttaaaga gtataccaaa agctattgct aaacatccat
    36301 atgtacttga acttaaatct gttcaaaaag acttaaagag tcagtattct cgtgcaaaac
    36361 agagtttaga gcaagctatg aaaaatgaaa tagtatttac agttaaagaa attgccaata
    36421 tctataaaaa tccagttatc tgccctttga ttaaaaatct agtatgggta tgtgatgata
    36481 atattggata tgtttataat gataagtcta agttctttat ttgcaatata gaaggtgaaa
    36541 cttataagtt gacggcaaaa aataaattaa aacttgcaca tccagttcat ttgtttgagt
    36601 ctggtaagtg gagtgagttc caacatgatt tatttagtaa gcaaattgtt caaccattta
    36661 aacaagtatt ccgtgagtat tatagaataa acagtgatga attatctgaa aaaaatatat
    36721 ctcgcagata tgctggtcac caaattcagc caaagaaaac tgtagcactt cttagaaatc
    36781 agctttggac tgtcgattat gaagatggat tacagaaagt attttataaa gaaaatatag
    36841 tggcaaagat gtttgcactt gcagattggt tttcaccagc agatatagag cctcctacta
    36901 ttgaaacagt agaatttttt gatagaaaaa cttataaaag cttagaatta acttctatac
    36961 ctccaattct attctcagaa gttatgcgtg atatagacct tgttgtaagt gtcgctcatg
    37021 ctggtggtgt tgaccctgaa actagtcatt ctactgtaga gatgcgtata gcaattgcta
    37081 aagagctttt attattatta aaaattaaca atgtcaattt tgaaggctct catgctctta
    37141 taacaggtaa tttaggtgag tattctgtgc atatgggttc tggtattgta cataaaaaag
    37201 gaatgggtgc tttgaatata cttcctgtac attctcaaag tagaggtaag atattccttc
    37261 cattcgctga tgaagaccct aaaactgctg agattatttc taagatacta ttgcttgctg
    37321 aagataataa gattaaagac cctagtatct taagtaattt ataagtataa aaactatctc
    37381 taaaatatat tggactttct taaaaaatta gaaagtccaa tatatcctac actataattt
    37441 atataaatta aatcataatt acaaataatt tttacataaa cttctcttat acattatctt
    37501 tacagtactt attgtattac acaaaaagaa tttttgttct cttttcacta gaatctttta
    37561 accctataaa atctatacag aaaatctttt tctagagatt tgtttaatat ggcattttct
    37621 tgaaaattta taaaccttat ctctaatgaa gatttattta taggtataac cttatgtata
    37681 aatcttagat ttactatgta ttgtctatga gtcataaaga aattaggagg taggcgtttt
    37741 tttaaatcat caaagttctc ataaaacttt attatatctt catctttagt gtgtagatta
    37801 attgctcttg aatttttctc aaacataact atatcatcaa aattaatcat atgtatctgc
    37861 ttccttattt tatagacaaa cagagtgtct tctataaaat tattattagc aattttatga
    37921 gagttaatat ggtctattgc tatgttaatt gaatcaagta ttttttcttt tttaaatggc
    37981 ttaactacaa aatctatagg atgcacttta aagctttcaa gactataatc agcaaagccc
    38041 gttacaaata ctatatgtat atccttatct atagaaagaa tttctttagc agaatcaatc
    38101 ccatttttat atggcatatc aatgtcaata atcaacaaat ctattttctc gttttttata
    38161 aactcaattg cctctatacc atccttaact tttttaacat aatctacata tccagattct
    38221 ttaactatat ttgctatatc atctcttaca attttatcat catcgcaaat caaaactttt
    38281 atcacagact ttctcctcct aactaataat attttggtat taaaattaca aactcagtta
    38341 aaaaatcatc actatttaca agtatactat aattatactt atctaatata tttttaacta
    38401 ggtgtagccc ataacccctg tcttgccctt ctttagtgct aaaaccatcc caaaatatac
    38461 tttcaacatt atgtattttg gggccattat tttttatgtc tataatatac tgaaattcat
    38521 cttcatatgc atgtaatctt atatacttct tttctttttt tatagtagac aaagactcta
    38581 ttgcattatc tattatgttt aatagtactg aactcatatc attaggttca atttctaaat
    38641 tatccataaa atcttgaatg tagacatcaa aagatatatc cttttcaaga cattcctcgt
    38701 attttaatga agctatagca tctaaataag atacttttcc ataccttgaa ttttttgatt
    38761 taaaactatt tgatatattc attatatatt ctttggcatc ttcatctagc ccagtattta
    38821 atagagaata caccacttgc aaatgtttca aatagtcatg tttttgactt cttaatttta
    38881 tgattatatt atctttatga tttattttac tatctagttt ttgtagttta tattcctgtt
    38941 cattattatt atttatcaat cttataactt taaagcctgt ataaaaactt aaaataatta
    39001 aaaccaaaaa aagtataata tttaatactc cttcaagtaa aaaactatat cctaacctta
    39061 attgtatata tttagtgccc tccataaata aaatcaatgc aaaacacgat ataaaaaatg
    39121 ttaatatgcc tctttttaat atgtctaatt ctttgtaaaa tttatactta ttcattataa
    39181 gattgctaaa tataaacaaa gttagtatta ctataggaaa tacaaaattg ttatccatga
    39241 ttcccctccc ctctacaatt tatataggga ttatatcatt ttaaaaaaat tatgtaaaaa
    39301 gttaaaattt acatacagat taattaaact tgtaaaaata cagatgaatt tgtacaatta
    39361 actacttaaa atttgaaaat acttactttg atatatattt tagataaata tataattgat
    39421 ttagttaaga aaggaagtgg taaatatgaa agataatttg aagatagtac taaggtacat
    39481 aaaaagttat aaagctagat ctttagcaat tgctctgagt ataattcttg ctacatcact
    39541 tattgttggt attggtactc tttcaagaag tgctcagcaa gcaagtgtag atagatttag
    39601 gcgtgaactt ggacctgacc atgtatattt taaggatata aagagaaatc agctagaact
    39661 cataaaatct agcaaagata ttaaaaattt aggtataaca tctcactatg gttatacaga
    39721 tattaatgaa aaattaccta taaacataga atatgtgaat aaaaattatt tgactagtca
    39781 ttctaaactt ataaaaggtc actttccaaa agctaataat gaggtggtag ttgaaaaatg
    39841 ggttttaaat agtatgggtt taaaaccaga aataaatcaa gacattactt ttaaattatt
    39901 caaaaaagaa aaaccagaga catttaaagt tgtaggaata cttgaagata gagctattca
    39961 gaaaaaacag ggtatgtgtg aaattttttt gaatatcaat gagtcaaaat tggataactt
    40021 ttcttatgtt tatgttgaat ttaatgaagg tagtgatata aatacaaaca taaataacat
    40081 agtaaaaaat actatgttgg ataagaatag tgttggaaaa aataaaacgc ttatagaagc
    40141 tactatggaa aatggtactt tagatggctc aagtaaatat attgcaattg ctatgagtat
    40201 attttctggg attgtaatat atagtatcta tgttatatca atataccaaa gagtacagga
    40261 atatggtatc ttaagagcta ttggagctac caactttagg atttttaaac ttatgtttta
    40321 tgaacttttc atacttgctt taatagcaat acctacaggc atatgtacag gtatggttgg
    40381 tgcacaaata tttaataaga ctgcgggaaa tattcaattt gaaggagatg taagggtcac
    40441 accttttgtt attccagata aagtaatctt tctagcaatt ggatgtataa tactgacaat
    40501 actaattata agtttcttta cgtatttaaa aataagaaga atttctccta ttgatgccat
    40561 aagaaaaact tttggaactg ataaaaatat aagtaaagtt aattctttaa tatcaaaatt
    40621 aacttcaaat atgtcagcaa caaaatctat atctactaag aatatcctta gaaataaaaa
    40681 gggatttatt ataattatat tatccatgag tataggaggt ataatggtca taaaggagaa
    40741 ttacaaatat tctttttcag atacacaaaa caaagatagt caagaaagag catatatgaa
    40801 tgcagatttt attctatcaa attttacttt caagttaaat gaaaattata aagctaattc
    40861 taacgcagat ataaatggtc ttaatgataa tcagatagat aaaataaaaa atatagatgg
    40921 aattgacaaa gtcaaaactg ctagcatctt gaatacaaga atagaattag ataaaataaa
    40981 taaattagat tactacaaca ttataagttc atcaccttat tataatgagt ttcctatatt
    41041 gacaaaaaat aagactactg gcaaatatac gctagcgcaa agattaagag gttataatga
    41101 tgagatgata aattcattag agaaatactt agtgagtgga agtataaact tagaaaaaat
    41161 gaaaaaggaa aacctggcta tagtatatgt cccacaaatt tctaaaacaa ataaatacag
    41221 caagagttat attcctggtg taggaacgtc cgttgtagac ataaaagtag gagatactat
    41281 aaaagtcaaa tatccaaaag gtgaaataga tacagatctt tatatgagac aaaaagataa
    41341 ttatgaatat ctagaatatg aatttaaagt tggagctatt gtgagctatc cttttgcaga
    41401 taataattca tactctggag atgaaggtat agatgtaata acgagtagcg aatatctaaa
    41461 aaaattaact ggtgtaaata aatataacac agtttatata gatcttaaag aaaatgcaaa
    41521 cataaaaaaa ataaatacac ttttaggtaa aataggaagt gaagttcctg gaactacaac
    41581 agtggatatg tcagtggaaa aagaaaactc tgataatatg acagctagag ctatgattta
    41641 tgcttatggc atagtagcag ttatgttcat aattagtgta tttaatataa taaataacat
    41701 aagttataac ttaacttcaa gaactagcga atttggaatg ttgagggcta ttggcatctc
    41761 tgatagaaga tttaaaaata tgatactcta tgagggaata ttatatggaa cactctcaag
    41821 tgtaattact attgtagcag gtcttataat acaatttaga atgtattaca cgtataattt
    41881 tgtatcatat ggattaggat tttcaataga ttacaaaata tatatcatag ttattttagc
    41941 aaatataatt gttggtattc tagctacata tattccatta aggaaaataa ataaaattag
    42001 tatagttgaa tctataaaca tcacagaata ggaggggtaa ttatgcctat attggaaact
    42061 attaacttag gtaagattta tggaaaaaag gaaacatctg tacatgctct aaaggatgca
    42121 aatttaaaaa taaataaagg cgaatttgtt gctataattg gaccaagtgg aagtggaaaa
    42181 agtacatttc tacatttagt aggtggtctt gaaagaccaa gtaatggtac tataaaggta
    42241 gatggtaaag atatatgttg tctttcagat aaagaattag caagatatag aagacaaaag
    42301 gtaggatttg tctttcaaca gtataactta attcctgtat taaatgtaaa ggaaaatata
    42361 gaattaccac taaagttaga taataaaaaa atcgataaag aatacattga tgaccttata
    42421 aatttattgg gtcttaatga aaggaaaaat catcttccga atcaattatc tggtggtcaa
    42481 caacaaagag ttgctattgc acgtgcttta tctgcaaaac caagtataat cctagcagat
    42541 gaaccaacag gaaatttaga cagtaaaact acagaagaag tcatggattt actaaaaagt
    42601 tctataaaaa aatataacca aacacttata ataataacac ataatgaaaa tattgctaga
    42661 aaagcagata gaattatatc tataatagat ggagagctta aattaacact ctaaaactat
    42721 ttatagaaaa gaggatttaa atgttattga ttttaggtaa tttaaattta aacttggatt
    42781 ttgactacag aataataaga gaagaaaatg acgatgtaga tatattcata gatattaatt
    42841 atcgtagctt agatatagat actgatggtt ctaacttatt taattctaga atacaatttc
    42901 catttgtaag agcattgatt ctaaggctaa ataagaacaa tcaatgtatg acaattcatt
    42961 tgttaagaga catagattta ttctctgctt ttgccaactt tgaagtggat tatactgact
    43021 ctataataaa tataaaaaat caaaatgaga aagtaatttt aaataaattt atcaaaaaat
    43081 agtaaggggg ccttaatagg ccccaattct ttttcttttc taaacttatt atatcatgat
    43141 aaataaatta gtcaatagaa ttttctgaat atttttataa ttttttcata aagtttttta
    43201 tatattatgc tttaaaatta tatgtaataa aaaaattttc cgactaattt taaagttaat
    43261 tgattaaatg cacatattca aaattaccat taaattactg actttttatc atttccattc
    43321 agaatctaag ttataatcta ttatttataa ctatttatac gtgtctttaa taaaactcta
    43381 tcttattcaa atctaataat ttatatacaa acccaataga acctatgctg tacaatgaat
    43441 attatttacc ataataaact tttagatata tctcttttaa ttcactcatc aatggatatc
    43501 taggattagc acctgtacat tggtcatcaa aagcttgttc aaccatagca tccagtcctt
    43561 ctaagaattt agattcttct accccagcct cttttatagt cttaggaaga cccacttttg
    43621 cctttagttc atctattgct tttataaggt tatctacttt ttcttggtct gtatttccct
    43681 taattcctgc aaaagaagca aattcagcat atctttgtat acaatcagga tatctgtatt
    43741 gtgagaatgc acccatctta tttggagctt ctgcacaatt aaacttgatt acttcattta
    43801 ttaataatgc atttgcaaca ccgtgttgaa catgatggaa tgcacctaat ttatgtgcta
    43861 aagagtgaca tattcctaag aaagcatttg caaaagacat tcctgccata gtagatgcca
    43921 ttgccatttt ttctcttgct tctttatcat tctttccatt tttatatgca cgaggtaaat
    43981 attcaaatat tattttacct gcttgtagtg ctaaaccatc agctggttca gttcttaaca
    44041 tagatacata tgcttctagt gcatgtgtta aagcatcaac acctgatgac gctgttagtc
    44101 ttggtggcat ctccatcatc atatcagcat caattatagc catgtttggc attaattcat
    44161 aatctgctaa tggatatttt acgcctgaat cttggtcagt tataactgca aatggagtta
    44221 cctctgaacc agttcctgct gaagttggga tagcagcaaa gtatgctttt tcacccatct
    44281 tagggaatac atatactctt tttcttatat ccataaatct catagctaag tcttggaaat
    44341 ctacttctgg atgctcatac attacccaca ttatcttacc agcatccatt gcgctacctc
    44401 cacctacagc tattattaaa tctggttgga aatcagccat agctttagcc ccttcttttg
    44461 cacaagctaa tgttgggtct ggagctacat caaagaatgt agtgtgctta attcccatct
    44521 catctaataa atcagttaac ttcttagtgt atccattatt atataggaaa gagtctgtta
    44581 ctataaatgc tttcttttta tccataacat ccttaaattc tcttgctgct acacctaaac
    44641 atcctttttt gaaataaact ttttctggtg ctctgaacca aagcatattc tctctccttt
    44701 cagctactgt cttgatatta agcaaatgtt ttactcctac attttcagat actgagtttc
    44761 caccccaaga accacaacca agagtaagag atggtgccaa tttaaagtta tataaatcac
    44821 ctataccacc ttgtgatgat ggagtattaa ctaatactct acaagtcttc atagcattta
    44881 caaacttttc tactctctct ggatgattta tagagtctgc atataatgaa gatgtatgac
    44941 catatcctcc atctgcaact aatttttctg ctttggcaac tgcatcttca aaacttttag
    45001 acttgtacat tgcaagtaca ggagaaagtt tttcatgtgc aaaaggttca tttaattcaa
    45061 cagattctac ttcaccaatt attattctag ctgtttttgg aacttctaca tcagccattt
    45121 tggctattac gtgtgctggt tgtccaacta ttctggcatt taatgaacca tcaattatta
    45181 ctacttttgc tactttttca gtttcttctt tatttaggaa atgtgctcca cgatatttaa
    45241 attctttttt aacttcttca tatatttttt ctggtactat tacggcttgc tcggaagcac
    45301 atatcatacc attatcgaat gattttgata ctaagattga acttacagcc atttttatat
    45361 ctgcactctc atctattatt gcaggcacat ttccaggccc aactccaaca gctggtttac
    45421 ctgaagagta tgcagattta accattccag gtccaccagt agctaatata aggtctgcct
    45481 ctgccatgac aacattagtt aattcaatcg aaggaacatc tacccaacct ataagacctt
    45541 ttggtgctcc tgccttaact gctgcatcat gtacaactct agctgcttct atagtacagt
    45601 ttttagctct tggatgggga gagaaaatta taccatttct agtttttagt gctattaggg
    45661 atttgaatat tgcagttgat gtaggattag ttgttggtat aactgctgat aaaacaccta
    45721 ctggctcagc aattcttgta aaaccaaatg cttcatcttt ttctatgaca ccacaagttt
    45781 tagtatcttt ataagcatta tagatatatt ctgaagcaaa atggtttttt ataaccttat
    45841 cttcaacaat acccatacca gtctcttcca ctgccatttt tgctagaggt attctttgtt
    45901 gattagctgc agttgaagca gctaagaaaa tcttatctac ctgttcttgc gtaaatgttg
    45961 caaattcttt ttgtgccaat ctaatttctt ctaatctcat tcttaatgtt tcaacactgt
    46021 tgacaatttc aggactacat acttcaacat ttgatttttc tactaccttt tcttttttct
    46081 ccatactaaa aacctcccta aaataattat gcatctctaa tttttataag tcttaaatgc
    46141 atcatctata tttttaactt atacttaaaa taattttata gatttcatct ctatacctta
    46201 gattttattt attttactct cttaggtcaa gaagtttttt tctgaatatt ttgtcaattt
    46261 attttttaac aaatatatta ttttttttac ttttactaaa attttttatc taaatgagat
    46321 atatttcatt tgaataatt
//
LOCUS       DAEPXF010000008        46230 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain HB27
            SAMN13376389-rid12479423.denovo.009, whole genome shotgun sequence.
ACCESSION   DAEPXF010000008 DAEPXF010000000
VERSION     DAEPXF010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13376389
            Sequence Read Archive: SRR10518432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 46230)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 46230)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001069624.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001069624.1
            Genome Coverage       :: 315x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/15/2021 01:54:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,165
            CDSs (total)                      :: 4,076
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 89
            rRNAs                             :: 10, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 10 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 19 of 69
            Pseudo Genes (incomplete)         :: 44 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 10 of 69
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..46230
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13376389-rid12479423.denovo.009"
                     /strain="HB27"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="China: Hebei"
                     /collection_date="2017"
     gene            complement(471..553)
                     /locus_tag="KRL67_000415"
     tRNA            complement(471..553)
                     /locus_tag="KRL67_000415"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(517..519),aa:Leu,seq:caa)
     gene            872..3520
                     /gene="polA"
                     /locus_tag="KRL67_000416"
     CDS             872..3520
                     /gene="polA"
                     /locus_tag="KRL67_000416"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HBH1521321.1"
                     /translation="MEKKLIIIDGNSIINRAFYALPEMNNKEGLKTNAIYGFTTMLFK
                     MIDIYKPTHISVAFDRKAPTFRHLEYKEYKAGRKGMPDELAEQLQPLKDLLDKFKINR
                     LEIDGYEADDIIGTVSKKAENNGYKVYIVTGDKDAIQLASDNTTTLITKKGVGEVEEY
                     NFNSVIEKYEMTPTQFIDLKGLMGDKSDNIPGVPGIGEKTGIKLIKEFSSIENLIENT
                     DKLKGSVKKKIEENKEIAIQSKRLATIIRDVPIEVDLDSMVFGDYDKDELLDKFRYFG
                     FTSLLSRLVDLVDSDDIEHVEEKIEILKLKDTEKFIDEVNKKGQVILKTVRGQGNILE
                     KNIIYIFISVDGEKIYYVDSNEVSKLDSIFSNNEIKKYGYNLKDDYISLKPYNINLEN
                     MYFDITIAEYLIDSTSSTSYDCSAIAMKYLSKKVKSKEELLGKGVKSKNYEDLEFEDL
                     AEYMGQIICTVKETVPMMERSLMDMSMDGLFYHVEMPLVEVLGHMEYEGIKVDKSILD
                     ELSVEFKEIIATLEKEIYELSGEPFNINSPKQLGVILFEKLELPIIKKTKTGYSTNAD
                     VLEKLRDKHPIIDKITEYRQIVKLNSTYVEGLLNIINPESNRIHSSFNQTITTTGRIS
                     STEPNLQNIPIKMEMGRKIRKVFIADDNCKLVDADYSQVELRVLAHMSQDENMIEAFK
                     HHEDIHTKTASQVFNVPMEDVTSELRGAAKAVNFGIIYGKSDFGLADDLNIPVPKAKE
                     YIESYFAKYHKIKEFMDTTVEKASEDGYAVTILNRRRYIPEIKSSNFMERNRGKRFAM
                     NAPIQGSAADIIKIAMINVHKKLEENNLKSKLILQVHDELIVEAIDDEIDIVKKIVKE
                     EMENAVNMDVHLDVDLNVGSSWYETK"
     gene            3529..4131
                     /locus_tag="KRL67_000417"
     CDS             3529..4131
                     /locus_tag="KRL67_000417"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HBH1521322.1"
                     /translation="MLILGLTGGIGCGKSSLSNIFRNLNISIVDADIISRKIFEDKLL
                     LEKVFVHFGQSIKNDDGTLNRKALGKIVFSDEEKLKELNNLTHPRIREKIISEIEELR
                     KKGENIVVLDAAILVESGFLDMVDKLLVVTCKQEVQISRIQKRDNCSKQEALSRINSQ
                     MSQEEKSKYGDYIIDNSGTITELESKAHKFIEYMKENWRE"
     gene            4124..4678
                     /locus_tag="KRL67_000418"
     CDS             4124..4678
                     /locus_tag="KRL67_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HBH1521323.1"
                     /translation="MNSKKVLILSIFIILFGALLMESKVIHKVLYPKKYSEYVEKYSK
                     EFNLDENIVYSVIKAESKFNSSAVSKKEAKGLMQILDITRDWGAEELNLKNVDIFDPE
                     TNIRLGCWYLSKLYKEFGKLDLVIAAYNGGSGNVKKWLENNEYSKDGENLHDIPFKQT
                     SKYVEKVKNNYEHYNKIYGKKGKN"
     gene            4678..6393
                     /locus_tag="KRL67_000419"
     CDS             4678..6393
                     /locus_tag="KRL67_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBH1521324.1"
                     /translation="MKRIKVLTVVLAITLMVAGCSNTKTKQSSSDSSLQSQSSIDAKY
                     INLTMVTPKTINPITNTDKSVGYIMNLVYDSLFTIDENYNVIPQLVKEYNIAQDGMSI
                     DIKLKDAKWHDGKNVTSNDVNYTIGLIQKSADSPYNEFTKNIASVNIKSDKDFTIKFK
                     ARYAFSIDSLIFPIVSQNHLDSKDVNDKNNNMIGNGKYKIESYTEREGMVLSVNKDYY
                     EEVPKTMKNIKVGMVPNEEARTSMVMALDSDITNVTLNDLSKFQEKEFNITRYQGRDY
                     ECVLFNYNNPFFKDVNFRKAIAHSIDKDRIISEGYMDDATPVNFPLNSKSKYYNSEMK
                     DLEFNKDKAIECLAKVEYANINSVNQNDNKVKNQKKSAKKNSKKLTPEEEAKAKKAEE
                     DRIKKEAEEKKNREKEEVKKSLSEINLKIIVNRDNSERIKTANIINENLKAIGINSTV
                     NQLSDKDMENALNSKNYDLALVGWKLSIIPDASSIIASSGYTDDKLNGYMSALTSSTS
                     EIETKKAYKDVQTYIKDNVTFISLAVRNNYIVSNSRLKGEITPNDFDVYEGISNLDIK
                     SNESN"
     gene            6466..6678
                     /locus_tag="KRL67_000420"
     CDS             6466..6678
                     /locus_tag="KRL67_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HBH1521325.1"
                     /translation="MKKKLLVEGMSCGHCVNHLKTALTEDIDGIEVLDVDLENKCASV
                     DMKDDVSIEKLKEVIAELGFELKGIE"
     gene            6879..7478
                     /locus_tag="KRL67_000421"
     CDS             6879..7478
                     /locus_tag="KRL67_000421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH1521326.1"
                     /translation="MESRGYKEDLKKMILDMAHKELESYVSQKGAKKYFEGNLDKIIN
                     NNIKKFNQSIGLNRVYLKMLKKCAYQESVTSISRTIKKEALFKSKKELFAFSEYLKLD
                     INKRLSYNQILRKISRYIYYNRSSYAQKYVVFKRGDEEYLLEPEKIKDELISSYRSKT
                     REDMRSIAKLLDIEVEDDYNAEDIRKKVINYIIKEKLKS"
     gene            7706..8077
                     /locus_tag="KRL67_000422"
     CDS             7706..8077
                     /locus_tag="KRL67_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HBH1521327.1"
                     /translation="MKFNFCHNNLNVTNLEKSLDFYKKALGLKEVKRKEAEDGSFILV
                     YLGDGITSHTLELTWLRDWDRPYNLGDNEFHLALEVEEFDEAKKLHKDLDCICFENES
                     MGIYFIADPDNYWIEILPKNH"
     gene            8209..9483
                     /locus_tag="KRL67_000423"
     CDS             8209..9483
                     /locus_tag="KRL67_000423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HBH1521328.1"
                     /translation="MKKAIAALGIGAVAVSVSSINASALEKGTVTASALNIRSGPSSD
                     CDKVAKLYKGKTVEILEKSNGWYKVRVSSSVVGWGSAKYISTSGSSEGTSNQNNPTSS
                     GTTISGNGKVNVSSRLNVRSGAGTNYSLVGKANNGDVVKLLEQSNGWYKIKLSNGVTG
                     WASSQYISKTSEDVGTNNSSNSNSTNNSDKKPSSEESIEGKNGKVTSAVSLNVRSGPG
                     TSYSIIGKLNGGDVVELKAKNNGWYKVKLSSGTIGWVSASYISETSEGTKEKPNSSSN
                     QNSQSNSNSKPSFTGNSDKSTAKGSTIVDFAYTLIGIPYQWGASGPDKFDCSGFTQYV
                     FKHSVGVSIPRVSREQANFGSAISMGNYAPGDLVYFDTDGDGTTNHVGIYVGNSKFIH
                     CSGTQTNPNKVKVDNLTSSYWSKVLLGARRFV"
     gene            9594..10538
                     /locus_tag="KRL67_000424"
     CDS             9594..10538
                     /locus_tag="KRL67_000424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH1521329.1"
                     /translation="MNRRKTIYRGFNNNRHKIDLRKYVITIACLCLIGYYSYTKIKDS
                     KILEYVSAKIPFLNNSSDITYKDISDELNSIKKGKKSKSQTNSDDKQETNPEKAVNNT
                     KEPEEVKLATIEGWDMYTIQVAAIDNNDDLKKIQTSLVNNDIPFSVMEKDGVKKIQTY
                     SSFDENDVRKQISSVRKVFPDAFLSHLDAPMLSLEYTSNYAYIESISKELNKLITNFK
                     EESSFWSNAENNVDMEKYNTILTNRKAISQNISKEAEKIDYSEMRLFKDNLMEYVKNV
                     NEKIDTASKAANEEKYSVSKSLLLSSMQEYSMFINSIK"
     gene            10754..12091
                     /gene="rsxC"
                     /locus_tag="KRL67_000425"
     CDS             10754..12091
                     /gene="rsxC"
                     /locus_tag="KRL67_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxC"
                     /protein_id="HBH1521330.1"
                     /translation="MKLLTFKGGIHPPYRKEYSNTKALEKAQAPKIVYIPLQQHIGAP
                     AKPIVEVGDEVKFGQKIGEQQGFVSCNVHSSVSGKVIAIEQHEVPGGSAQCVVIENDF
                     KEELHESVQPKGQLEDLSKEDIVGIIKEAGIVGMGGATFPNHVKVSPPPDSKAEVVIL
                     NGAECEPYLTADHRLMVENPEDVVFGLRALMKVLDVKKGFIGIETNKPDAIEAIQNVA
                     KDYSEIEVVGLQVKYPQGAEKQLIYACTGKEVPSGGLPIAAGAVVDNVATAAQIAKSI
                     KTGMPLVERITTITGSCIKEPKNLITKVGTLVSEIIEQCGGFKEDKKIGKVIMGGPMM
                     GIAQYTTEIATNKGSSGILCLDEEESRTPDIQNCLRCGRCTDVCPSFLQPLFISAYSL
                     KDDYDTAEYHRAMDCIECGSCSFICPARRPLLQSIRSAKREIGAKRRKQAAQK"
     gene            12114..13091
                     /locus_tag="KRL67_000426"
     CDS             12114..13091
                     /locus_tag="KRL67_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit D"
                     /protein_id="HBH1521331.1"
                     /translation="MENKLIVSSSPHVRSNEDTSYIMKQVIIALLPAAVAGVYFFRLN
                     ALSAMFFCILGTVGTEFLYQKFTKQKSTIGDFSAVVTGLLLAFNVPASLPWWMCLVGG
                     IFAILVVKMVFGGIGCNFVNPALAARAFLLASFPVAMTAWTQPGVNWIGKNLDAVTTA
                     TPLSFLKNGAAGLADLSSNGISLADMMIGNIGGCIGETSAILLLLGGVYLMYKGIINY
                     VIPVFYIATVFILTFLLGGFNITFAIYQLFAGGLMLGGFFMLTDYTTSPMTKKGQIIY
                     AVLAGLITTVIRMYGGYPEGVSYSILLVNCLAPLIDKFVRNRVFGEVAK"
     gene            13091..13660
                     /locus_tag="KRL67_000427"
     CDS             13091..13660
                     /locus_tag="KRL67_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit G"
                     /protein_id="HBH1521332.1"
                     /translation="MNSMVRLGGTLLAISAIAALALGATNQVTAPVIEQRNIQANNEL
                     RKAVLPEAKEFKEMDKSTYKDLGDGLIAEVYEGLDGSEVVGYTLKAKPSGYGGEIEVM
                     VGISSEGQVTGVDIGNMSETAGLGAKAKDDAFKGQYKGKTAEPLEVAKGSTTADNQIL
                     AISGATITSTAVTTGVNAAIDVFNSALNK"
     gene            13673..14266
                     /locus_tag="KRL67_000428"
     CDS             13673..14266
                     /locus_tag="KRL67_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit E"
                     /protein_id="HBH1521333.1"
                     /translation="MNLAKVFKNGLIDENPTFVQVIGMCPTLAVTTSAINGIGMGLST
                     AAVLICANLVISLIRKITPDKIRIPIFIVVIATFVTIVGMLLKAYVPALDKALGIYIP
                     LIVVNCLILARAESFAFKTGAMPSIVDGVGQGLGFTVALTIIGAVRELLGNGSLFGMT
                     LFGASFQPVLIFILPPGAFLTLGFLFAGFNKLRSKKA"
     gene            14281..14856
                     /gene="rsxA"
                     /locus_tag="KRL67_000429"
     CDS             14281..14856
                     /gene="rsxA"
                     /locus_tag="KRL67_000429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxA"
                     /protein_id="HBH1521334.1"
                     /translation="MNLILLFLSIVLVNNVITSQFLGICPFLGVSKKVDTAVGMGVAV
                     TFVLTLASIITYFIQILLVKTGTGFLQTIAFILVIASIVQFVEMVIQKMSPSLYQALG
                     VYLPLITTNCAVLGIALVNVQKSYNLVETIINGFGAGAGFTLAIVIFAGIRERLELAD
                     IPEAFKGFPITLISAGLMSIAFLGFTGLIKL"
     gene            14872..15843
                     /locus_tag="KRL67_000430"
     CDS             14872..15843
                     /locus_tag="KRL67_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit B"
                     /protein_id="HBH1521335.1"
                     /translation="MVILTAVLVLGIMGLIFGIVLDFASKKFAVEVDERVEAILGVLP
                     GANCGGCGFPGCGGLANAIVEGNAPVNGCPVGGADVGAKVGEIMGISAEAGEKQVAKV
                     ICKGTCSSAKDKYEYEGISDCRAANVLNSGAKMCKFGCLGLGTCKDACKFDAISIVDG
                     IAVIDEEKCVNCGKCKEVCPKGIIITKPESQEVVVECNSKEFGKAVKEKCTAGCIGCG
                     MCVKACKFDAIIFEDKIAKIDPNKCVGCMQCVAKCPTKVISGDITKKKKVTIDQELCV
                     GCTVCKKQCKFDAIEGELKEKHKVDADKCVGCHLCMEKCPKKAIKIL"
     gene            16063..16647
                     /gene="maf"
                     /locus_tag="KRL67_000431"
     CDS             16063..16647
                     /gene="maf"
                     /locus_tag="KRL67_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation inhibitor Maf"
                     /protein_id="HBH1521336.1"
                     /translation="MNIILASASPRRKEILENTNVRFDIIKNEIDEIILEGETPKHLV
                     MRLAFEKSMSVASEHNEDVVIGADTVVVLDNAILGKPKDESCARDMLKRLSGREHQVI
                     TGISLINLCEDKKVIDYVISNVKFKTLSEQDIEDYLKTNESFDKAGAYGIQGYGALLV
                     EEIRGDYFNIVGLPISRLGDLLKKYFSINLFYGV"
     gene            16692..17351
                     /gene="radC"
                     /locus_tag="KRL67_000432"
     CDS             16692..17351
                     /gene="radC"
                     /locus_tag="KRL67_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HBH1521337.1"
                     /translation="MAPEERPREKMLAKGVKSLSNAELLAILLRTGNKNKNAIELANY
                     IINRDIQGIRHLEDMTIEELCNIDGIGLSKSTQIKAALELGSRVASFKPIKYKIRNPW
                     DIQRYYMDSLRYLKKEVFKAVLLNTKNEIISDVDVSIGTLSSSLVHPREVFKEAIRRS
                     ASKIIVMHNHPSGSVEPSREDKNITSRLIKCGEIIGIEIIDHIIIGDGLYFSFKENMI
                     I"
     gene            17472..18482
                     /locus_tag="KRL67_000433"
     CDS             17472..18482
                     /locus_tag="KRL67_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HBH1521338.1"
                     /translation="MTKDMGIDLGTANTLVYIKGQGIVVREPSVVAIRDDSKEVLAVG
                     EEAKKMIGRTPGNIVAIRPMKDGVIADFDITQSMISYFIQKAADKKGVVSPRIAICVP
                     FGVTEVEKRAIEEAARQAGAKDAFLIEEPMAAAIGAGLKVEEPEGNMVVDIGGGTSEI
                     AVISLGGIVTAKSIRIGGDEFDESIVAYVKKEYNLMIGERTAENVKINIGSTFKDDEE
                     INMQIRGRDLISGLPKTIEICSTEVREALKEPVSSIVDAIKSTLERTPPELASDIMEN
                     GIMLTGGGALLRGLDKLITQETGMSVQIAETPLDCVALGTGKSVEDQEIFEKVLMMNT
                     KN"
     gene            18492..19385
                     /gene="mreC"
                     /locus_tag="KRL67_000434"
     CDS             18492..19385
                     /gene="mreC"
                     /locus_tag="KRL67_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HBH1521339.1"
                     /translation="MALRFDKNKNEKKRINVKVIATGVVAITLIGIVGISIGKFSSGS
                     PINLGVASDAITSVGKGINDGFSFIKNGFKDVANFKDNSKKVKKLEEENEKLKKDMIA
                     LNAKLDKTESLEELKKTLNFVQEEYKATSISASVVGKNDGNWYESFVIGAGKNSGVKK
                     ESIVMNGSGLVGIVYEVSNNYSKAISLLDSKASVSFKLAKDANAKGTITQNTTLDNKD
                     SYNSKGYLQGYMFDSSYNVIQGDIITTSGLGFFPDGIPIGEVEKVVDDKDKSLKYVVV
                     KPYVDFKNINDVVVIEPRNIG"
     gene            19400..19888
                     /gene="mreD"
                     /locus_tag="KRL67_000435"
     CDS             19400..19888
                     /gene="mreD"
                     /locus_tag="KRL67_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HBH1521340.1"
                     /translation="MKKVLLCLLGIVLMTIENSITNYIDIFGISFNLVLIYVTIISLY
                     LDELEGGIIAAIIGLIKDITVGGIFGVNALILFIIAYAIGYMRDQLYKGSYIITFVLI
                     FIGSLIDSIVNIGTSSIIYQSHNISTLFVKGLLVAPIVNSLIGLVIYRLSKKAVLKLK
                     ED"
     gene            19900..22878
                     /locus_tag="KRL67_000436"
     CDS             19900..22878
                     /locus_tag="KRL67_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HBH1521341.1"
                     /translation="MEENKQVDRLKIIKIVMSVIFLAILVKIIYMTTFKYEYYNELAE
                     NKTYKKLAIEAPRGEIKDRYGRLLAGNKNLFTVQVSGNDINKKDANKHSRANEISLKL
                     INLLERNGEEYVDEFPIYVENGKYYYTYDRDIREYKSENGIPNDYNAKESFYYLVDKL
                     ISAGILSQEDKRLDATRLQAKLNENGYYPPILVSKWMFTAERDKRDWLASYKIKETKL
                     SAKEAFEKVRNSDALEIDKSLSDEDARKIMVVRDLIKSKGYSQYNPVTIAKDVGETTI
                     AQIEERAMDLVGVSIAVEPVRYYPNGSLASHMLGYVGKMPSTQIESYLQKGYETGDMV
                     GLAGVEKSNESRLRGTDGYKMVKVDALGRISKEIESKKPKSGDTVYLTLDKDLQEVSD
                     NALKQIIEVASKGGTFKSKFGDKPISAYAGKAQSAALIAIDVKNGEVLASSSYPNYDP
                     NKFAKGISTEDYKALQPKNPNDLLAGSPLLNLVTQGEFQPGSSFKMVTSMAALENGLD
                     PNFTINDPGVIMLGKKSFGDYVWNHGRGNHGMTNLYKAIQESCNIYMATIGTGKTWPG
                     GKSIGIDMNANKILEYAKLFGLDQNTGLQDEVEERAGKVPSTEDKLKSTQALLKSNLE
                     REMANDFVDITREKNPKEYEKRINEIVSWAAEKKTPGRVETMNRLKKMNVKEDRIEDV
                     ADLAVFSYFNFAKWSTADTFNLAIGQGENAYTPAQISRSVAAIANGGNLVELSVVDRA
                     VSSDYSSVKINDQKKVEKIPFKNPDNLKELTKGMKLVARQGTAKSAFADFPIDVAAKT
                     GTAEKSGKIPTDNEYEYLKSHMSSYNVNLNDAIKLADKMKAEKEKELSLAKEKEIKKK
                     LENKDLKDEERKKLEEELEDGVKVRLEDTDKVNSSYLRKAIKELNPKITDDQIDRFKQ
                     DYGSFTWTVAFAPADDPEIAVVCVIPQGDSSVFSLLPTREVIGTYMGLEPTNSKNDNK
                     TNDVNNSSDENINFESQINR"
     gene            22976..23659
                     /locus_tag="KRL67_000437"
     CDS             22976..23659
                     /locus_tag="KRL67_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinC"
                     /protein_id="HBH1521342.1"
                     /translation="MSLREICSQELVEFKGNKRGIIVNIKREAPFEEIQEKIINKLEA
                     YVGFFNGAKISKINSDCLTDMEILELKEGITSRFDVEFVEDQKIEENSNFPTKYVNTL
                     RSGENIEFEGDVVILNDMKPGSKVLSKSNTVVMGDINAGAKVVAGGNVFVMGKIEGFV
                     HAGAEGNEFAYVVAGNLNPKILQIADNIAEAPDDEENYESESEISPEIAFVSNGRIVI
                     ESYLSKLDK"
     gene            23683..24480
                     /gene="minD"
                     /locus_tag="KRL67_000438"
     CDS             23683..24480
                     /gene="minD"
                     /locus_tag="KRL67_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinD"
                     /protein_id="HBH1521343.1"
                     /translation="MSEVIVITSGKGGVGKTTTAANLGTALSLENKKTVVVDADIGLR
                     NLDVVMGLENRIVYDIVDVVEGTCRLKQALIKDKRFDNLYLLPAAQTRDKNAVSVEQM
                     IDLCEKLKESFEYIIIDCPAGIEQGFKNAVAGADRAIVVTNPEISAVRDADRIIGLLE
                     ANEIKEIRLVINRIRNDMVKRGDMMDKQDIIEILAIDLLGLVPDDESIIISTNKGEPA
                     ILDSKSLAGQAYKNIAKRILNEEVPLLDLEVEDGFFGRLKKMFSMAK"
     gene            24498..24782
                     /gene="minE"
                     /locus_tag="KRL67_000439"
     CDS             24498..24782
                     /gene="minE"
                     /locus_tag="KRL67_000439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division topological specificity factor
                     MinE"
                     /protein_id="HBH1521344.1"
                     /translation="MLDLFRVFSNEAKTSKSVAKERLKLVLVHDRVDCSPQLLEMIKT
                     DILKVIANYAEIEDDGLEIKMSKCRGEHDDKPVSALVANIPLKNIKDRCM"
     gene            24867..25997
                     /gene="rodA"
                     /locus_tag="KRL67_000440"
     CDS             24867..25997
                     /gene="rodA"
                     /locus_tag="KRL67_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein RodA"
                     /protein_id="HBH1521345.1"
                     /translation="MKKININYKSTIKLIKQLDWKLIVTVLAIFIFGLVILSSATHAN
                     STGSYNQLIKQGLAFVLGIGMIIVILFFDYNLLGRYYKALYIISLVLLAIVLLPGIGT
                     VKGGARSWINLGPLDLQTSEIVKLTFVLSYAKILESKKDKLNTLKEVMPVVVYSLPFI
                     GLLIAQPDLGTGIVFCCMIFAMLFTAGLSSKLIKRGIIILLVSMPLMYLMMADHQKIR
                     IEAFLNPEDVTLKGNYQVMQSLIAIGSGGVTGKGLYNGSQNQEDFLPVQDSDFIFAVV
                     GEELGVIGMAVLIILFMIFLLRLLAIARDAKDFYGTLIVVGVMGMFGYQIIQNIGMTV
                     ALIPVTGVTLPFVSYGGSSLLTSLANLGLVLNVCMRRKKINF"
     gene            26038..26451
                     /locus_tag="KRL67_000441"
     CDS             26038..26451
                     /locus_tag="KRL67_000441"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HBH1521346.1"
                     /translation="MNIALVAHDQMKNTMVGFCIGYESILKKYGLYATGTTGKRIMDE
                     TELNINRLASGPLGGDQQIGSLIVTQEIDLVIFLRDPLTSQAHETDIQALIRLCDVYH
                     VPIATNLASAEIFIKALDRGELSWREVRKSKSQRV"
     gene            27101..28135
                     /locus_tag="KRL67_000442"
     CDS             27101..28135
                     /locus_tag="KRL67_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
                     /protein_id="HBH1521347.1"
                     /translation="MKKIRKLATKLLIATMVLTSCMSVTVYGMTAKDVTAKTPKSYVA
                     GTNSIYGPKLSQAQLNSVAQATADFMNKKITKNMTTDAKIMVAYNHIKNNTTYVDWNA
                     AEGANTAYTLVTKKGACSGMCRSMKALCDAMGIECYYVHSIANDHQWNLIRFGDGRLY
                     HVDIDANKSAGKDIIYKSLAHPLPFDKNAYPAVGAVKVQKPASPGKHTMLADQEGAIL
                     NEVYGNNMKPQNKMYEGVPCSSIDSSINTIKFTSSNSNLDPSKHTISVIGFMKTPYGM
                     VALAPDSNPVSYPFEYDKPFSLDFTGKNRSAAELSKYCKKNNIIIKVEIGETASNSGR
                     GMVAYARYVN"
     gene            complement(28232..29425)
                     /locus_tag="KRL67_000443"
     CDS             complement(28232..29425)
                     /locus_tag="KRL67_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FprA family A-type flavoprotein"
                     /protein_id="HBH1521348.1"
                     /translation="MSKVFEVKKDIYFTGVVDEGLKVFDIIMETEFGTTYNSYLIKDE
                     KTVLFDTVKANFKDEFLSNLSEVTDIAKIDYVVIHHTEPDHAGSLKYLLDINPNIEVY
                     CTKAAKLYLDGQINRPFNCHVIKDGEILNIGKRNLRFITAPFLHWVDTMFTYIEEDKT
                     LLTCDAFGCHFASVDAEVVNSEDYLKSAKHYYDCIVKPFAKHVLSAVDKVVGLNIEFD
                     TILTSHGPMLTKDPMAAVKRYVEWSTEAVNTTNQNQVSIFYLSAYSNTLEMAKKIKEG
                     LDKEGAKAELYDLEDMTLTEMHDTLVVSKVILLGSPTINKTMVKPMWDLFSVIDPMAN
                     QGKIAGVFGSFGWSGEGITMAETLLKSMSFKMPVESLKKKFFPSEETLKECMAFGAEF
                     AKLVK"
     gene            29744..31588
                     /locus_tag="KRL67_000444"
     CDS             29744..31588
                     /locus_tag="KRL67_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03960 family B12-binding radical SAM
                     protein"
                     /protein_id="HBH1521349.1"
                     /translation="MNKVDLKKILKKVEKPARYFGNEINSIHKDTSNSELIRYAHCFP
                     DLYEVGMSHLGSHILYDVINKDEDVFCERVYSPAVDMENMMREKSIPLFALESREPIT
                     NFDFVTFTLQYELSYTNILNILDLANIPILKEERTLEDPFIMVGGPCAYNSEPLADFV
                     DVVILGEGEEVNLEVVNEYKEWKKNKTTREDFLYKISSIEGVYIPSFYDVKYNEDGTV
                     QSVTPNREGITKNPTKRIIKDVETVDYPEKLIVPYIDTVHDRIVLELFRGCTRGCRFC
                     QAGMIYRPIREKSVKRLKEILDKLVKNTGYDEISLSSLSTSDYSKLSELTDYLVDEYA
                     SNNIGISLPSLRLDNFSMEIADKIQQVRKSGLTFAPEAGTQRLRDVINKGVTEEDLEN
                     ATERAFEMGWNSVKLYFMIGLPTETYEDLDGIAKLAYKVIDIYRKVNGGKLKRSFSVT
                     VSTSTFVPKPFTPFQWHGQDTTEEVINKQRHLVNKLRNNNIKYNYHDSKTSLMEAVVA
                     RGDRRIGKVIYDAFRLGAKFDGWAEHFNLDIWKEAMEKNNLSIDFYAHRNRNYEEVFP
                     WDHIDVGISKKFLIREDENAKKEKITSDCRHNCNGCGINIHDIGRGLC"
     gene            31590..32291
                     /locus_tag="KRL67_000445"
     CDS             31590..32291
                     /locus_tag="KRL67_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2344 domain-containing protein"
                     /protein_id="HBH1521350.1"
                     /translation="MSKIIRVKFKKEGDMIYISHLDLQRLLQRAFRRAEINLSHSQGF
                     NPHPKMSYGNALALGTESQGEYVDIEIEEDDLSVEEFLNKVSIQLPNGIDFIKAKEID
                     RKTPSLSSVIDYGEYLFNIDLKRPMTKEFVKRKVIDFMNNKEIIITKKNKKGKMVEVD
                     IRPMIRTFDVLNLEDEHITLTATIATGSKTNLNTNILIPKILEMFELDIDPLDVDILR
                     RDLYVLEDGELVTPM"
     gene            32309..33730
                     /locus_tag="KRL67_000446"
     CDS             32309..33730
                     /locus_tag="KRL67_000446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rne/Rng family ribonuclease"
                     /protein_id="HBH1521351.1"
                     /translation="MKKIVIESLIGSQKTAVLEDERLTELFVEDNLNKKTVSNIYRGI
                     VKKVIPGIEACFVDIGFKKLAYLQLKKGSTIKSGQDILVQINKEEIGTKGAKLNTEIS
                     ISGRYIVYIPSNDRTTISNKITDEKERFRLKKITKAVNKENLGLIIRTEAQGCTHDEI
                     KKDIEELKLKYENILKEYKLGIGPKLLYKSLDFATKYVKDNVNDDIESIITNSYDKYS
                     ELKSILRSIDKTYVDKLCLEENRDVFDLYRIESKIEKLLNKKVWLKSGGYLIIEKTEA
                     LTVIDVNTGKFIGTGKLDETVYKTNLEAAKEIVRQLRIRDIAGIIIIDFIDMHKKKHQ
                     KEILHILEEEFNKDKRKAEVLGMTKLGLVEVARRREKESIDKYYLMSCPCCDGEQTIK
                     SVHYILDSIEKEIMRISEHTVYKNIMVEFNDFIFEQIKGYYMDIIDKIGEKYNIKISL
                     NANSTLKHNKTNVIFDKIVDNKM"
     gene            33831..34142
                     /gene="rplU"
                     /locus_tag="KRL67_000447"
     CDS             33831..34142
                     /gene="rplU"
                     /locus_tag="KRL67_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012063435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HBH1521352.1"
                     /translation="MYAIVKTGGKQYKVSEGDVLFVEKLEANAGDVVTLNEVLACSKD
                     GELKLGSPVVEGASVQAKVVEQGKAKKVIVFKYKAKKDYRRKQGHRQSYTKIVVEKIN
                     A"
     gene            34157..34474
                     /locus_tag="KRL67_000448"
     CDS             34157..34474
                     /locus_tag="KRL67_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal-processing cysteine protease Prp"
                     /protein_id="HBH1521353.1"
                     /translation="MIKVKYYYNDDFLLQGFCLKGHADFAEIGYDIVCAAVTSNAIAV
                     INSLDKLQKIEFEKVVGEEGHIECIVKDAHIKDAQLLLNHFQLAVEEIKREYPKNIKI
                     LKK"
     gene            34488..34778
                     /gene="rpmA"
                     /locus_tag="KRL67_000449"
     CDS             34488..34778
                     /gene="rpmA"
                     /locus_tag="KRL67_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HBH1521354.1"
                     /translation="MLNMNLQLLASKKGVGSSKNGRDSISKRLGVKRFDGQLVTAGSI
                     IVRQRGTKIHPGTNVGKGSDDTLFALVDGTVKFERKDKKRKKVSIYPVAIAE"
     gene            34889..36166
                     /gene="obgE"
                     /locus_tag="KRL67_000450"
     CDS             34889..36166
                     /gene="obgE"
                     /locus_tag="KRL67_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000469293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HBH1521355.1"
                     /translation="MFIDKARIFVKAGNGGNGSVAFRREKYVPAGGPDGGDGGRGASI
                     IFEVDLGLRTLMDFKYQKKYQAQNGGDGSKGKRAGKNGENLVLKVPAGTVIRDEATGL
                     VLADLKKEGDTAIVAKGGIGGKGNQHFANAVRQAPAFAKSGTDGEERWITLELKMIAD
                     VGLLGFPNVGKSTFLSVVTKAKPKIANYHFTTLTPNLGVVQTKFGDSFVLADIPGIIE
                     GASEGVGLGHEFLRHVERTKVLIHIVDISGLEGRDPIEDFDKINDELKLYNEKLSKRP
                     QVVVANKFDILEDESKFEKFKSELEGRGYTVFKMSAATRQGIDEVIAYVSKMLKEVED
                     VELVSEEEMYRPELDIGTEEELSIDIEDGVYVVTGKALRRIMYSVNFDDMESLQYFQK
                     AMESQGVFDRLREMGIEDGDVVKIYELEFEFYN"
     gene            36218..36586
                     /locus_tag="KRL67_000451"
     CDS             36218..36586
                     /locus_tag="KRL67_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhbY family RNA-binding protein"
                     /protein_id="HBH1521356.1"
                     /translation="MLKGKQRAYLRSLANTLKPTTQIGKEGVTESFLEQLDGMLRTRE
                     LVKVTILENAGLDTKETANAVCEALRAEFVQAIGFKFTIYKRNIEEPKILFPGHEQAK
                     AKTKNNNVTKKGKPTKRAVR"
     regulatory      36678..36784
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            36887..38092
                     /locus_tag="KRL67_000452"
     CDS             36887..38092
                     /locus_tag="KRL67_000452"
                     /EC_number="1.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HBH1521357.1"
                     /translation="MKIAILGYGTVGSGLIDLIENNTSKRDIEVVGILVKNKEKHKYK
                     KYFDKITTDIEDIFDKDIDILVEVIGGLNPAFDYVTRALNKKIHVVTANKDLLAEKGS
                     DLIELANLNNVSIKFEASVAGGIPVLKPLIESLEGNNIKSINAILNGTCNFILSKMYD
                     ENLPYDVALKQAQELGFAEVNPDADVLGYDSARKLSILSTLSYGKIVYWKDLLLEGIT
                     SIDEKDIEYAKKLNCKIKLVARSKYESGEVSGFVRPALVDNNNMLSKIDNEFNVVILV
                     GDSVGELSFVGKGAGRGATGSAVYADIIDIIDNRNSNIKSFSKGKLDLSGLIEDECSA
                     VVRFGGYNNKDGILECLDKYVDSYDIIDDEELAIFVSAKSEHEIDRFLEVVKRNNYSE
                     SAKKLLKID"
     gene            complement(38293..38859)
                     /locus_tag="KRL67_000453"
     CDS             complement(38293..38859)
                     /locus_tag="KRL67_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HBH1521358.1"
                     /translation="MATRPIEIDEYKKIMELLHTGFTYSENGVEKRFRKNPKVALALM
                     LEANLGLRISDILRLKIGNFKGNMLEINEKKTGKLQYRPVNKNIIESINEHARKYNLK
                     SNDYLVNIKTKAIQKQLRIICKHLNLYNISTHSFRKLYATTQFEKSNNNLELVKELLN
                     HSSVATTQRYIRVTQQAINEASENFFIG"
     gene            complement(39127..40386)
                     /locus_tag="KRL67_000454"
     CDS             complement(39127..40386)
                     /locus_tag="KRL67_000454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation integral membrane protein YlbJ"
                     /protein_id="HBH1521359.1"
                     /translation="MKKNKIIAFFAPAIIVGVLVLGIILYPNEAIKSAREGFSIWSNV
                     LVPSLLPFIIGANLIVDLKIVDIIGFIINPITRLVFNVSGKSALAFAISTVSGYPVGV
                     SLASEFRSNGQISKHEAQRLVSFCSTSGPLFIIGSVGTGMFQNSNLGYLMILCHYLGT
                     ILVGLFFRNYGNENLPKTKLDLKTNIKNVINIRNSSGNGFFVLFGNAVFNGVNTLLTV
                     GGFVIVFSVVFKILSLFNVISLIASVIYLPLSFLGVSKELCQAFVSGLFEITIGCNKV
                     SSITSSPEILRASLASFLIGFSGLSILAQCCTFLSKTDIDLKLYILSKFAHGVLAAIF
                     IFILYPIANSAVLVSSFADTYNVIYNNLIWFYYLSYYDTILQIVVIIYLISAVFIAKK
                     HMKTLSTNGKLMRYKNTFFIKKFSRKK"
     gene            40511..41224
                     /locus_tag="KRL67_000455"
     CDS             40511..41224
                     /locus_tag="KRL67_000455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FadR family transcriptional regulator"
                     /protein_id="HBH1521360.1"
                     /translation="MFNSIASKKVYEQVIEQIQYKILNGELKKGDKLLSERELSEQMN
                     VSRTSIREAIRVLETMGVIESRQGEGNFICTNIEKTLIEPLSMIFKLNNGTLEDILEL
                     RIILEIEIAKLASKRITSSEIIELKHIIDEMRVETNKKDNNRVLVLLDQKFHNKLATL
                     SKNYLIQSLFMTASKLFDGFIEDAREKIIAEPFNENILLKQHEAIYNAVVENDVELAC
                     EKAKEHMDFISKNYRKNEN"
     gene            41342..42616
                     /gene="larA"
                     /locus_tag="KRL67_000456"
     CDS             41342..42616
                     /gene="larA"
                     /locus_tag="KRL67_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-dependent lactate racemase"
                     /protein_id="HBH1521361.1"
                     /translation="MVKLKVPYSKQGMILEIPDERFLGVLESKSDNYIEHKTQEEIVE
                     ESMNNPIGSNSLEELVKGKKNVVIITSDHTRPVPSRITMPIILRRIRNVNPEIDVKII
                     VATGFHRPSTREELIYKMGEEIVDNEDIVMHISTDDKSMCKVGILPSGGDLYINKLAY
                     EAELLIAEGFIEPHFFAGFSGGRKSVLPGIASAKTIMYNHCSDFIDSDNSRTGKLNNN
                     PIHEDMVYAAKVAKLAFILNVVIDKDKKIIASFAGDVEKAHTKGCRFVTALSKVNSIK
                     SDIVVTTNGGYPLDQNIYQSVKGMTAAEATCKDGGVIIMIAACNDGHGGQSFYENVAN
                     ADSPKQLLDKIRSVDRVDTIPDQWEFQILARILSKYTVIMVTDKCNPEMIKKMHMKHA
                     FNFKEALEMATSIVGKKSKVVVIPDGVSVIVK"
     gene            42633..43421
                     /locus_tag="KRL67_000457"
     CDS             42633..43421
                     /locus_tag="KRL67_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003457975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HBH1521362.1"
                     /translation="MEILTCIKQVPGTTSVEVDETTGVLKRDGVDSKMNPYDLYALET
                     ALRIKEEKKANLKVLSMGPPQAKKVIEESFMMGADEGALISDRRFGGADVLATSYTIS
                     QGIKKMGKVDLIICGKQTTDGDTAQVGPEVAEFLDIPHVTNVTKLIEVRDESIVVEID
                     MPNDLQVCEIEYPCLITVEKDIFQPRLPSFKLKLNTKDREIPVYSLDDFEDKDENNYG
                     LNGSPTQVVRIFPPKPNTDKNIVRGSADELSFALVNKLEELKLV"
     gene            43435..44655
                     /locus_tag="KRL67_000458"
     CDS             43435..44655
                     /locus_tag="KRL67_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HBH1521363.1"
                     /translation="MSKIVVNQDKITDLKRILEICPFGAIEEKSGIVEISAGCKMCKL
                     CVKSGPKGAFEFIESSKVQINKDEWRGIAVYVEHHNGNIHPVTYELIGKAREMASKIK
                     QPVYCVFVGKDIKDKCSNLISYGVDEVFVYDEDEFKDFRIEPYSKAIENFINKIKPTI
                     VLVGGTTLGRSLAPRLAARFRTGLTADCTILDIQSNTDLDQIRPAFGGNIMAHINTPN
                     NRPQFATVRYKIFSVPEKIENTTGKITLCKVEKKELKSKIKVLSVKEKNKEVGLEEAE
                     VIVVASRAIKKQEDMEMIYKLADKLNAQVAGTRPVIEAGWIDAKKQIGLSGRTVKPKL
                     IITCGVSGAVQFVAGMQGADYIVAINKDDKAPILDVAHLALIGDIYDIIPKLIEKIEN
                     NKDNNQKYMASAAK"
     gene            44666..46057
                     /locus_tag="KRL67_000459"
     CDS             44666..46057
                     /locus_tag="KRL67_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HBH1521364.1"
                     /translation="MYKLVDKKDIDFLIDTCGEENVLVGSDINEDFSHDELGGIEKYP
                     EVLVNVLETEQVSKIMKYAYKNNIPVTPRGQGTGLVGAAVAINGGIMINLCKMNKILE
                     VDYENLTLTVEPGVLLMTIGQYVQDRDLFYPPDPGEKSATIAGNINTNAGGMRAVKYG
                     VTRDYVRGLEVVLPNGEIINVGGKVVKNSSGYSIKDLLVGSEGTLGIVTKAILKLLPL
                     PKKSISLLIPFPDLSMAIETVPKIIKSKSIPTAIEFMERDVILAAEEFLGKKFPDNTS
                     DAYLLLTFDGNSTEDIEKEYEKVANLCLENGALDVFISDTQERNDSIWSARGAFLEAI
                     KASTTQMDECDVVVPRDKIAEFIRYTHELQDKLKIRIKSFGHAGDGNLHIYILKDGMD
                     DNTWKIRLKETFDYMYKKSRELSGQVSGEHGIGYAKKEYLHESNSDAYMMLIKNIKLA
                     FDPKNILNPGKIY"
BASE COUNT        18201 a         5188 c         8709 g        14132 t
ORIGIN      
        1 atttaattat tttacaatga aaaatctaaa ctacttttta aatagcttcc tcttccatta
       61 atttatttgt atattactga tttaattctt caagtaacta attgattgtt acaacacttt
      121 atcctaaact ctagaaactt tataaactac atctctagca tttggtaaat ttccatctgg
      181 aacacctaca gcaataccaa atggtacaga ataaccttct agtatatcaa gtagttctct
      241 gtatttttta tttgtatcaa aatttttaga gattgggaaa aaatcaatat gataagaagt
      301 atggatttat taagtgaata cataaaaaat cctactttgt ttagatatat tttaggagtt
      361 caagaagtta tgactatggt atttatactt atacaaggct agaaataaag atgaaagaat
      421 attacaataa aaaaaaactc tttagtatat actaaagagt ttttttttat tggtgccgct
      481 gatgggactc gaacccatat ggtttcccgt acgattttga gtcgtatgcg tatgccagtt
      541 ccgccacagc ggcttatatt ttaaatccga actacttgta tatcctacca ggttttccaa
      601 gaatatgcaa gaggaatatt gaatacaaaa tactcatcaa actgtaaaac attataaaaa
      661 cttatgaatc aacacagttg attttgaatc cattgtattt accaattcca ccactcgggc
      721 atgtcatgta tattattata acaaaaaaat atattgtttg caacatattt aattttaata
      781 tactattatt attatgggaa tattaaataa cactatattg gtatataata tatatgttac
      841 aaaaatagat aatttttagg agtgatataa tttggaaaaa aaattaatta taatagatgg
      901 aaattcaata ataaacagag ccttttatgc tctgccagaa atgaataata aagaagggtt
      961 aaagaccaat gcaatatatg gatttacaac aatgctattt aaaatgatag atatatataa
     1021 accaacacac ataagtgtag cctttgacag aaaagcacct acatttagac atttagaata
     1081 taaagagtat aaggctggaa gaaagggaat gccagatgaa ctggcagaac aattacagcc
     1141 attaaaagac cttttagata aattcaaaat aaatagattg gaaatagatg gctatgaggc
     1201 agatgacatt ataggaactg tttctaaaaa agcagaaaat aatgggtata aggtatacat
     1261 agtaactgga gacaaagatg ctattcaact tgcttcagat aatacaacta ctcttataac
     1321 aaaaaaagga gttggagaag ttgaagaata caactttaat tctgtaattg aaaaatacga
     1381 gatgactcca acacaattta ttgatttaaa aggtcttatg ggagataagt cagataatat
     1441 acctggagta cctggaatcg gtgaaaaaac aggtataaag cttataaaag agttttctag
     1501 tatagagaat ttaatagaga atacagataa gcttaaaggt agtgtaaaaa agaaaataga
     1561 ggaaaataaa gaaatagcta ttcaaagtaa aagacttgca acaattataa gagatgtgcc
     1621 aatagaagtg gatttagata gtatggtttt tggcgattat gataaagatg aacttttaga
     1681 taaatttaga tactttggat ttacaagtct cttatcaaga ttagttgatt tggtcgattc
     1741 agatgacata gaacacgtag aagaaaaaat agaaatatta aaattaaaag atacagaaaa
     1801 atttatagat gaagtaaaca aaaaaggaca agttatttta aaaactgtta gaggacaagg
     1861 taatatactt gaaaaaaata taatatatat ttttataagt gtagatggag aaaaaatata
     1921 ttatgtagat tcaaatgaag tgtcaaagtt agatagtata ttttcaaata atgaaattaa
     1981 aaagtatgga tataatttaa aagatgatta tatatcttta aaaccatata atataaactt
     2041 agaaaatatg tattttgata taacaatagc tgagtactta atagattcaa cttcatctac
     2101 ctcatatgat tgtagtgcaa tagctatgaa gtatttatca aaaaaggtta aatcaaaaga
     2161 agaattatta ggaaagggtg ttaaatctaa aaactatgag gatttagaat ttgaggattt
     2221 agcagagtat atgggtcaaa taatatgcac agttaaagaa acagttccta tgatggaaag
     2281 aagtctaatg gatatgagta tggatggtct tttttatcat gtagagatgc ctttagtaga
     2341 agtcttaggt catatggagt atgaagggat aaaagtagac aaaagtatat tggatgaatt
     2401 gagtgtagag tttaaagaaa taatagctac actagaaaag gaaatttatg aattatcagg
     2461 agagccattc aatataaatt caccaaaaca attgggtgtt atattatttg aaaaattaga
     2521 gcttccaata attaaaaaaa ctaaaactgg atattcaact aatgcagatg ttttagaaaa
     2581 attaagagat aagcatccaa taatagataa gataactgag tatagacaaa tagtaaagtt
     2641 aaattctact tacgttgaag gacttttaaa tataataaat ccagaaagta atagaataca
     2701 ttcatcattt aatcaaacta taacgactac aggaagaatt tcatctacag aaccaaatct
     2761 tcaaaatata cctataaaaa tggaaatggg tagaaaaata agaaaagtgt ttattgcaga
     2821 tgataactgt aagttggttg atgccgatta ctcacaagta gagctaagag ttcttgcaca
     2881 catgagtcag gatgaaaata tgatagaagc atttaaacat catgaagata ttcataccaa
     2941 aacagcttca caagttttta atgttcctat ggaagatgtt actagtgaat tgagaggtgc
     3001 agctaaagct gtaaactttg gtattatata tggtaaaagt gactttggtc tagctgatga
     3061 tttaaatata cctgttccaa aggcgaaaga atatatcgaa agttattttg ctaaatacca
     3121 taaaataaaa gagtttatgg acacaacagt agaaaaggct agtgaggatg gatatgctgt
     3181 aacaatttta aacagaagaa gatatatacc ggaaataaag tcaagcaatt ttatggaaag
     3241 aaatagagga aagagatttg caatgaatgc accaatacaa ggaagtgcag cagatataat
     3301 aaaaattgct atgataaatg ttcataaaaa attggaagag aacaatttga aatcaaaact
     3361 tatactacaa gttcacgacg aattaatagt tgaagcaatt gatgatgaaa tagatatagt
     3421 taagaaaata gtaaaagaag aaatggaaaa tgccgtaaat atggatgttc acttagatgt
     3481 agatttaaat gtaggtagtt cttggtatga gacaaagtag gtgattttat gttgatatta
     3541 ggacttacag gagggatagg ctgtggaaaa agcagcctat caaatatatt tagaaatctt
     3601 aacatttcta tagtggatgc tgatattata tccaggaaga tatttgagga taagttgtta
     3661 ttagaaaaag tatttgtcca ttttgggcaa agtataaaaa atgatgatgg aactctaaat
     3721 agaaaagctc ttgggaaaat agtttttagt gatgaagaaa aacttaagga gttaaataat
     3781 ttgactcatc cacgaataag agaaaaaata ataagtgaaa tagaagaatt gagaaaaaaa
     3841 ggagaaaata tagtagtatt agatgcagca atattggtag aaagtggttt tttagatatg
     3901 gttgacaagc tattagtagt tacttgcaaa caagaagtac aaattagtag aatacaaaag
     3961 agagataatt gtagtaagca ggaagcactt agtaggataa attcacagat gagtcaggaa
     4021 gaaaaatcca aatatggtga ttatataata gataattcgg gaacaataac tgaattagaa
     4081 agtaaagcac ataaatttat tgagtacatg aaggagaatt ggcgtgaata gtaagaaagt
     4141 attgattcta tctattttta taatcttatt tggagcacta ttaatggaaa gcaaagtaat
     4201 acataaagtt ttatatccta aaaaatattc agagtatgta gaaaagtatt caaaagaatt
     4261 taatttagat gaaaatatag tttacagtgt tattaaagcc gaaagtaagt ttaatagttc
     4321 tgctgtttca aaaaaggaag caaaaggatt aatgcaaata ttagacataa ctagagattg
     4381 gggagcagag gaactaaatt taaaaaatgt agatattttc gacccagaga ctaatataag
     4441 acttggatgt tggtatttaa gtaagttata caaagaattt ggtaaattag atttagtgat
     4501 agctgcatat aatggtggtt caggtaatgt gaaaaaatgg ttagaaaata atgaatatag
     4561 taaagatggc gaaaatctac atgatatacc ttttaagcaa acttcaaaat atgtagaaaa
     4621 agtaaaaaat aattacgaac attataataa gatatatggc aagaaaggaa aaaactaatg
     4681 aagagaataa aagtcttaac agtcgtttta gctataactc ttatggtagc tggttgtagt
     4741 aatactaaaa caaaacaaag tagtagtgat tcaagtttac aaagtcaaag ttctatagat
     4801 gctaagtata taaacttaac aatggtaact ccaaaaacta taaatcctat aactaatact
     4861 gataagtctg ttggttatat aatgaattta gtttatgata gtttatttac tatagatgag
     4921 aactacaatg taattcctca attagttaag gagtataata tagctcagga tggaatgagt
     4981 atagatataa agctgaaaga tgcaaagtgg catgatggaa aaaatgttac ttcaaatgat
     5041 gtaaactata ctattggttt aattcaaaaa agtgcagata gtccatataa tgaatttact
     5101 aaaaatatag cttctgtgaa tattaaatca gataaagatt ttacaataaa atttaaagct
     5161 agatatgctt tttcaattga tagtttaatt tttccaatcg tatctcaaaa tcatttagat
     5221 tcaaaggatg taaatgacaa gaataataat atgataggaa atgggaaata taaaatagaa
     5281 tcatatacag aaagagaagg aatggtttta tctgtaaaca aagattacta tgaagaagtt
     5341 ccaaagacca tgaagaacat aaaagtaggt atggtgccta atgaagaggc aaggacatct
     5401 atggttatgg ctcttgatag tgatataacc aatgttacat taaatgattt aagcaagttt
     5461 caagaaaaag agtttaatat aacaagatat caaggaagag attatgagtg tgtattattt
     5521 aattataata atccattttt taaagatgtt aattttagaa aagcaattgc tcactctata
     5581 gataaagata gaattattag tgaaggttat atggatgatg ctactccagt taattttcca
     5641 cttaattcaa aatcaaaata ctataatagt gaaatgaagg atttagagtt taataaagat
     5701 aaggcgatag agtgtcttgc taaggtagaa tatgcaaata taaactcagt aaatcaaaat
     5761 gacaataaag taaaaaatca gaaaaaatca gctaaaaaga actcaaagaa gcttacgcca
     5821 gaggaagaag caaaagctaa aaaagcagaa gaagatagaa tcaaaaaaga agctgaagag
     5881 aaaaaaaata gagaaaaaga ggaagtaaag aaaagtcttt ctgaaattaa cttaaagata
     5941 atagtaaata gagataatag tgagaggata aaaactgcaa acataataaa tgaaaactta
     6001 aaagctatag gaataaatag tacagtaaat cagctgtcag ataaggatat ggaaaatgct
     6061 cttaattcta aaaattatga tttagcatta gttggatgga aactatcaat tataccagat
     6121 gcaagtagta ttatagcaag tagtggatat actgatgata agttaaatgg atatatgtct
     6181 gcacttacaa gttctacgtc tgaaattgaa acaaaaaaag catacaaaga tgtacagaca
     6241 tatataaaag ataatgtaac atttataagt ttagctgtca gaaacaacta tattgtaagt
     6301 aatagtagat taaaaggtga aattactcca aatgattttg atgtatatga gggaatatct
     6361 aatttagata taaaatcgaa tgaatcaaat taaatgatta atttctattc aaaaagtaca
     6421 aactatatga taaaataccc ttatagggta taaggaggta attaaatgaa gaagaaacta
     6481 ttagtagaag gtatgagttg tggtcattgt gtaaaccatc ttaaaactgc attaacagaa
     6541 gatatagatg gtattgaagt tttagatgta gatttagaaa ataagtgtgc atctgtagat
     6601 atgaaagatg atgtatctat tgaaaaatta aaagaagtga tagcagaatt aggatttgaa
     6661 ctaaagggaa ttgaatagaa attaataaac taaaaaaaga cctctattgg gggagtaata
     6721 gaggtctaaa aatgggggag attgagtatg tttgatttta cattactatt atctcccctt
     6781 ttaaattaat atatacataa aaagtaaaaa aattttgtaa tgactcacat ttcttgagct
     6841 ttttgaatat actataaata gctaagggag ggagtatcat ggaatctagg ggatataaag
     6901 aggatttaaa aaaaatgata cttgatatgg cccacaaaga acttgagagt tatgtatcac
     6961 aaaaaggagc aaaaaaatac tttgaaggta acttggataa gattattaat aataatataa
     7021 aaaaattcaa tcaaagtatt ggtcttaata gagtatattt aaaaatgtta aaaaagtgtg
     7081 catatcaaga aagtgtaact tcaatatcaa ggactataaa gaaagaagca ttatttaaat
     7141 caaaaaaaga gctatttgct ttttcagagt acttaaaact tgatattaac aagcgacttt
     7201 catataatca aattttaaga aaaatatcaa gatatatata ctacaataga agttcatatg
     7261 ctcaaaaata cgttgttttc aaaagaggag atgaagaata tcttttagaa ccagagaaaa
     7321 taaaagatga attaatatca agctataggt caaagactag agaagatatg aggtcaatag
     7381 caaaactttt ggatatagaa gtagaagatg attataatgc tgaagatatt agaaaaaaag
     7441 taataaatta tattatcaaa gaaaaactta aaagttagag ttttaaagaa taagttctgc
     7501 caatttgcat gattaagtat aacttgggta taaatttttt gtgcatttga aagagcttat
     7561 tctttttttg acatattctt ttaaataatt taccaccatt tggtaattgg tcatcatcat
     7621 tcaaattttt aacttaatat gatttaaata aatttataat aatgatataa tataaaaaaa
     7681 ataaaaattg gagagtgatt tcaaaatgaa gtttaatttt tgccataaca atttgaatgt
     7741 tactaactta gaaaaaagtt tagattttta taaaaaagct ttagggctta aagaggtaaa
     7801 gcgtaaggaa gcagaagatg gaagttttat attagtttat ttaggagatg gtattactag
     7861 tcatacatta gaattaactt ggcttagaga ttgggacaga ccatataatt taggagacaa
     7921 tgaatttcat ctggctttag aagttgagga gtttgatgag gcaaaaaaac ttcataaaga
     7981 tttagattgc atatgttttg aaaatgaaag tatgggaatt tattttatag cggacccaga
     8041 taattattgg attgaaatac ttcctaaaaa tcattaattt taaggatatg tttataattt
     8101 ctttacaatt aataagagaa taagttatca ttaaattgta gggactaaaa atggataata
     8161 atgttatgaa tttatgacta taaaaatttg ttggggggag tgatagtagt gaagaaagct
     8221 atagcagctt taggaattgg ggcagtagca gtatctgtta gctccataaa tgcaagtgct
     8281 cttgaaaagg gaacagtaac agcaagtgcc ttgaatataa gaagtggacc aagttctgat
     8341 tgcgataagg tagcaaaatt atacaaggga aaaactgtag agattttaga aaagtctaat
     8401 ggatggtata aagtaagggt atcgagttct gttgttggat ggggaagcgc aaaatacata
     8461 tcaacaagtg gttcatctga aggtacatca aatcaaaata atccaacttc aagtgggaca
     8521 actattagtg gaaatggtaa agtaaatgtg agttctagac tgaatgtaag aagtggtgca
     8581 ggtactaact attcattagt tggtaaagca aataatggag atgtagtaaa attactagaa
     8641 caaagcaatg gatggtataa aataaaacta tcaaatggag ttactggatg ggctagtagc
     8701 caatatatct ctaaaacatc agaagatgtt ggaacgaata attccagtaa ttcaaatagt
     8761 actaataatt ctgataaaaa accatctagc gaggaatcaa ttgaaggcaa gaatggtaag
     8821 gtaacatctg ctgtgagttt aaatgtaaga agtggtcctg ggacaagcta ttctataata
     8881 ggaaaattaa atggtggaga tgttgttgaa ttaaaagcta aaaataatgg ttggtataag
     8941 gtaaagttat cgagtggaac aattggatgg gtgagtgcca gttatatttc tgagactagt
     9001 gaaggtacaa aagaaaaacc aaattcatca tctaaccaaa attctcaatc taatagtaac
     9061 tcaaagccat catttactgg aaattcagat aaatctacag caaaaggttc aacaatcgta
     9121 gattttgcat atactcttat tggaatacca tatcaatggg gagcttcagg acctgataaa
     9181 tttgactgtt ctggatttac acagtatgtt tttaaacatt ctgtaggcgt ttctattcct
     9241 agagtttcta gagaacaggc taattttgga agtgcaattt ctatgggaaa ttatgcacca
     9301 ggagatttag tttattttga cactgatggt gatggaacaa ctaatcatgt aggtatatat
     9361 gtaggaaata gtaaatttat ccattgtagt ggtactcaga ctaatcctaa taaggttaaa
     9421 gtggataatt taactagttc atattggtct aaagtattac ttggtgcaag aagatttgtt
     9481 tagtaagtac aatttcttat taaaaaatta gaagaaataa ttatttatat tcataaattg
     9541 tgactcttaa aaatatactt tattaataat ataattttgg gagggacaaa ctaatgaata
     9601 gaagaaaaac gatatacaga ggatttaata ataatagaca taagattgat ttacgtaaat
     9661 atgttataac gattgcttgc ttgtgtctaa ttggctatta ttcttataca aaaataaaag
     9721 atagcaagat attggagtat gtatcggcta aaataccatt tctaaataat tcatcagata
     9781 taacttataa agatatatct gatgaattaa attcaataaa aaaagggaaa aaatctaaat
     9841 cacaaaccaa ttcagatgat aaacaagaaa ctaatccaga aaaagcggta aataacacta
     9901 aagagccaga agaagtcaaa ttagctacta tagaaggctg ggatatgtac actattcaag
     9961 ttgctgcaat agataataat gatgatttga aaaaaataca gacatcatta gttaataatg
    10021 acataccttt ttcagttatg gagaaggatg gagtaaaaaa aatacagacc tattcctctt
    10081 ttgatgaaaa tgatgttaga aagcagataa gcagtgtaag aaaggtgttt ccagatgcat
    10141 ttttatcaca cttagatgca cctatgttgt ctttagagta tacaagtaac tacgcttata
    10201 tagagagtat atctaaagaa ttaaataaat taataacaaa ctttaaagaa gaatcaagtt
    10261 tttggtcgaa tgctgaaaat aatgtggata tggaaaaata taatactatt ttgacaaata
    10321 gaaaggcaat atcacaaaat atatcaaaag aagctgaaaa gattgattat tctgaaatgc
    10381 gtttatttaa agataattta atggagtatg taaagaatgt gaatgaaaaa attgatacag
    10441 cttcaaaagc agcaaatgaa gaaaaatata gtgtaagtaa aagtctgctt ttatcatcca
    10501 tgcaggagta ctctatgttt ataaattcta taaaataaaa tacagaaaat tttgacttta
    10561 gaaagctata tgaaaaggat tgtttaatta agcaatcctt ttcctaaatt tgacttaata
    10621 tagaggtgtt gatttataaa ataaattata aaaatacgga tattttttta acaattgtta
    10681 agatttttta atatatatag tatacttata gaaggcagaa aaaatgtttt tttatagata
    10741 agggggtaaa acaatgaaac tcttaacttt taagggaggg atacatcctc catatagaaa
    10801 agagtactct aacacaaagg cacttgaaaa agctcaagct ccaaagatag tttacattcc
    10861 tcttcagcaa catatagggg caccagctaa acctattgtt gaagttggtg atgaagtaaa
    10921 gtttgggcaa aaaataggtg aacaacaagg ttttgtttca tgtaacgttc attcttcagt
    10981 atcaggtaaa gttattgcta ttgaacaaca tgaagtacca ggtggttcag ctcaatgtgt
    11041 agttatcgaa aacgacttta aggaagaatt acacgagagt gttcaaccaa aaggtcagtt
    11101 agaggatttg agcaaagaag atatagtagg tatcataaaa gaggcaggaa tagtaggtat
    11161 gggtggagct acattcccta accatgtaaa agtatctcct ccaccagata gcaaggcaga
    11221 agtagttata ttaaatggtg cagaatgtga accatactta actgcagacc acagattaat
    11281 ggttgaaaat ccagaagatg ttgtatttgg tttaagagca ttaatgaagg tattagatgt
    11341 caaaaaagga tttataggaa ttgaaacgaa taaaccagat gctatagaag caattcaaaa
    11401 tgtagctaaa gactatagcg aaatcgaagt tgttggcctt caagtgaaat atccacaagg
    11461 agcagagaaa caacttattt atgcgtgtac aggcaaagaa gttccatctg gtggattacc
    11521 aatagcagca ggtgcagttg ttgacaacgt tgccacagca gcacagatag ctaagtctat
    11581 taaaacaggt atgcctttgg tagaaagaat cactacaata acaggtagtt gcatcaagga
    11641 gccaaagaac ctaataacaa aagttggaac attagtttca gaaataatag aacaatgtgg
    11701 tggatttaaa gaagataaga agattggtaa ggttataatg ggtggaccta tgatgggaat
    11761 agctcaatat actactgaaa tagcgactaa caaaggttct tcaggaattt tatgtttgga
    11821 tgaggaagaa tcacgtactc cagatattca aaactgttta agatgtggaa gatgtacaga
    11881 tgtatgtcca tcattcttac aaccactttt cataagtgca tattctttaa aagatgacta
    11941 tgacacagct gaatatcata gagcaatgga ttgtatagaa tgtgggtctt gctcatttat
    12001 ttgtccagca agaagaccat tacttcaatc tataagatca gcaaagagag aaataggagc
    12061 aaagagaaga aagcaagctg ctcagaaata aagaaagtta agggggaaaa acgatggaaa
    12121 ataagttgat agtatcatct tctcctcatg tgagaagtaa tgaagatact tcatatataa
    12181 tgaaacaagt tattatagca ctccttccag cagcagtagc aggagtatac ttctttagac
    12241 ttaatgcttt gagtgctatg tttttttgta tacttggtac agtaggtact gaatttttat
    12301 atcaaaaatt tacgaagcaa aaaagtacta taggagattt ttcagctgtt gtaacaggat
    12361 tattattagc atttaacgta ccagcatcac ttccttggtg gatgtgtcta gtaggaggaa
    12421 tatttgcaat attagtagtt aaaatggtat ttggtggaat tggatgtaac tttgtcaatc
    12481 cagcacttgc tgcaagagca tttttattag catcattccc agtagcaatg actgcttgga
    12541 ctcaaccagg tgttaactgg ataggtaaaa atttagatgc agttactaca gcaactccat
    12601 taagcttttt gaaaaatgga gcagcaggac tagcagacct ttctagtaat ggaattagtc
    12661 ttgctgacat gatgataggg aatattggtg gatgtatagg tgagacatca gcaatattat
    12721 tattattagg tggagtatac ctaatgtata aaggcataat aaattatgtt ataccagtat
    12781 tttacatagc aactgtattt atattaacat tccttttagg tggatttaat ataactttcg
    12841 caatatatca actgtttgct ggaggactta tgttaggtgg attcttcatg cttacggatt
    12901 atacaacttc gcctatgact aaaaaaggtc aaataatata cgctgtatta gctggtctta
    12961 taacaactgt tataagaatg tatggtggat atccagaagg tgtatcttac tcaatactat
    13021 tagtgaactg tcttgctcca cttatagata agtttgttag aaatagagta tttggggagg
    13081 tggctaaata atgaacagta tggtgagatt aggtggtact ttacttgcta taagtgcaat
    13141 agctgcatta gctcttggag ctactaatca agttacagct ccagtaatag aacaaagaaa
    13201 tatacaagca aataatgaac ttagaaaagc agtccttcca gaagcaaaag aatttaaaga
    13261 aatggataaa agtacatata aagaccttgg agatggtctt atagctgaag tgtatgaagg
    13321 tttagatggt tctgaagtgg ttggatatac tttaaaagct aaaccaagtg gatatggtgg
    13381 agaaattgaa gttatggtag gtatatcatc tgaaggtcaa gttacaggtg ttgacattgg
    13441 taatatgtca gaaacagcag gtcttggagc aaaagctaag gatgatgcat ttaagggtca
    13501 atacaaagga aaaactgctg aacctcttga agtagcaaaa ggaagtacaa cagcagataa
    13561 tcaaatatta gcaatatcag gtgcaactat tacatctaca gcagttacta caggagtaaa
    13621 tgctgctata gatgttttca atagtgctct taataaataa ggagggttaa ttatgaattt
    13681 agctaaagta tttaaaaatg ggctaataga tgagaaccca acatttgttc aagttatagg
    13741 tatgtgtcca acactagccg ttacaacttc agcaataaat ggaattggta tgggtctttc
    13801 aacagcagca gttttaatat gtgcaaattt agttatatct ttaataagaa aaattacacc
    13861 agataagata agaataccta tatttatagt agttatagct acatttgtta caatagtagg
    13921 tatgctttta aaagcatatg ttcctgctct tgataaggct cttggtatat atataccatt
    13981 aatagttgtt aactgtttaa tattagctcg tgcagaaagt tttgctttca aaacaggagc
    14041 tatgccttca atagttgatg gtgtaggtca aggtcttgga tttactgttg cacttacaat
    14101 aataggtgca gtaagagaat tacttggaaa tggtagttta tttggaatga cattatttgg
    14161 agcatctttc caaccagtat taatatttat attaccacca ggtgcattct taacattagg
    14221 attcttattt gcaggattta ataaattaag aagtaaaaaa gcatagtgga ggtgtaataa
    14281 atgaatttaa tacttttatt tttaagtatc gttcttgtta ataacgttat aacatctcaa
    14341 ttcttgggta tatgtccttt cttaggagtt tctaagaagg ttgatacagc agtagggatg
    14401 ggagttgcag ttacattcgt tttaactcta gcttcaatta taacttattt tatacagatt
    14461 ttattagtaa aaactggaac tggattctta caaacaatag cttttatatt ggttatagct
    14521 tctatagttc agttcgttga aatggtaatt caaaagatga gtccatcttt atatcaagca
    14581 ttaggagttt atttacctct tataactaca aactgtgcag ttcttggtat agctcttgtt
    14641 aatgttcaaa aaagttataa tttagtagaa acaataataa acggatttgg agccggagct
    14701 ggatttactt tagcaatagt aatattcgca ggtataagag aaagactaga attagctgat
    14761 ataccagaag catttaaagg tttccctata acgcttatat cagcaggttt aatgtcaata
    14821 gctttcttag gatttacagg acttataaag ctataaaaaa gaggggtgaa aatggtgata
    14881 cttacagctg tattagtttt aggaattatg ggcttaatct tcgggatagt actagatttt
    14941 gcatctaaaa agtttgctgt agaagtagac gagagagtgg aagctatact aggagtactt
    15001 ccaggagcca actgtggagg ttgtggattt ccaggttgtg gaggtcttgc aaatgcaata
    15061 gttgaaggca atgcaccagt taatggttgc cctgtaggtg gagcagatgt tggtgcaaaa
    15121 gttggagaaa taatgggaat aagtgctgaa gcaggtgaga aacaagtagc aaaagttatt
    15181 tgtaaaggaa cttgttcaag tgcaaaagat aaatatgagt atgaaggaat atcagattgt
    15241 agagcagcaa atgttttaaa ctcaggtgct aagatgtgta aatttggatg tttaggtctt
    15301 ggaacatgta aagatgcttg taaatttgat gctatttcta tagttgatgg aatagcagta
    15361 atagatgaag aaaagtgtgt aaattgtgga aaatgtaaag aagtttgtcc taagggtata
    15421 ataatcacaa aaccagaaag tcaagaagta gttgtagagt gtaatagcaa agagtttggt
    15481 aaagctgtta aggaaaaatg tactgctgga tgtattggct gtggaatgtg tgtaaaagct
    15541 tgtaaatttg atgcaataat atttgaagac aaaatagcta agatagaccc taataagtgt
    15601 gtaggttgta tgcaatgtgt agcaaaatgc cctacaaaag taatatcagg tgacataact
    15661 aagaaaaaga aagttactat tgaccaagaa ctttgtgttg gatgtacagt gtgcaaaaaa
    15721 caatgtaagt ttgatgcaat agagggagaa ttaaaagaaa aacataaggt agatgcagat
    15781 aaatgtgtag gatgccattt atgtatggaa aaatgtccaa agaaagctat taaaattttg
    15841 taatatggac aaaataagga tatggaataa tttccatatc tctaatgagt acataaaaca
    15901 gaggtatgga ataatttcca tacctctatt ttatgtatat ttattctaat ggtaataaat
    15961 ataacaatag tgtaacaaaa aaggttcgac atttctgttg aattttttac aatatagtga
    16021 tagactaaat aggtacaaat tacgtacaaa tgaggtgaat aaatgaatat aatcttggca
    16081 tcagcctctc caagaaggaa agaaattcta gagaacacga atgtaagatt tgatataatt
    16141 aaaaatgaaa ttgatgagat tatattggaa ggtgaaacac caaaacattt agttatgaga
    16201 ttagcttttg aaaaaagtat gtctgtagca tctgaacata atgaagatgt tgttattgga
    16261 gctgatacag ttgttgtttt agacaatgct attttaggaa aaccaaaaga tgagtcttgt
    16321 gcaagagata tgttaaaaag actatctgga agagagcatc aagtcataac aggtattagc
    16381 ttaataaact tgtgcgaaga taaaaaggta attgactatg taatcagcaa tgtgaaattt
    16441 aaaacgttat cagagcagga tattgaggat tatctgaaaa ctaatgagtc atttgacaaa
    16501 gcaggagcct atggtataca aggctatgga gctttgttag ttgaagaaat tagaggcgat
    16561 tattttaata tagttggtct tccaatttca aggttaggcg acctattaaa aaaatatttt
    16621 agtataaatc ttttttatgg agtgtgattt atctgaaaaa gtcatttaat tcaaccatta
    16681 aggtaaaaga aatggcacca gaggaaagac ccagagaaaa gatgcttgct aaaggagtaa
    16741 aaagcttatc aaatgctgag ttgttggcta tacttttaag aactggcaac aagaataaaa
    16801 atgctattga attagccaac tatataataa atagagatat tcagggtatt agacatttag
    16861 aagatatgac aatagaggag ctgtgtaata tagatggaat tgggttatca aaatcaaccc
    16921 aaattaaagc agctttagaa ctaggctcta gagtagctag ttttaaacct ataaagtata
    16981 aaataaggaa tccttgggat atacagaggt attatatgga tagcctaagg tatttgaaaa
    17041 aagaggtttt taaagcggtt cttttaaaca caaaaaatga gataatatct gatgtagatg
    17101 tatctatagg gactttaagc tcgtcattag tccatccaag agaggttttt aaagaagcta
    17161 taagaagaag tgcaagtaag ataatagtga tgcataatca cccttcagga agtgtagaac
    17221 catcaagaga agataaaaat atcacatcaa gacttattaa atgtggagaa ataattggaa
    17281 tagaaataat agaccatata attattggag atggattata ctttagcttt aaagaaaata
    17341 tgataatttg actttatatg tgataaaata ataggagaat gttttaaaca tgattcttaa
    17401 tagtaggaag gagtaaaatc atggctaagg aaaaaaagaa agaaaaaaaa ggttttttta
    17461 gttttaataa gatgacaaag gatatgggaa tagatttagg gactgcaaat acattagttt
    17521 acataaaagg acaaggtatt gttgttagag aaccatcagt tgtcgcaata agagatgata
    17581 gtaaagaggt attagcagta ggtgaagaag caaaaaaaat gataggtaga acaccaggaa
    17641 atatagtagc tataagacct atgaaagatg gagtaatagc tgattttgac ataactcaat
    17701 caatgattag ctattttata caaaaagcgg ctgataaaaa aggtgttgta agtccaagaa
    17761 tagctatatg tgttcctttt ggagttacag aagtagaaaa gagagctata gaagaggctg
    17821 caagacaagc aggagctaaa gatgcattcc ttatagaaga gccaatggca gcggctatag
    17881 gggctggatt aaaagttgaa gaaccagaag gtaatatggt agttgatatt ggtggaggta
    17941 cttcagaaat agctgtaata tctttaggag gaatcgttac tgctaaatct ataagaatag
    18001 gtggagacga atttgacgag tctatagtgg cttatgtaaa aaaagaatat aaccttatga
    18061 taggtgaaag aactgctgaa aatgtaaaaa ttaacatagg ttctacattt aaagatgatg
    18121 aagagataaa tatgcaaata agaggtagag atttaatctc aggattacct aaaacaatag
    18181 aaatatgctc tacagaagtt agagaagcat taaaagaacc tgtaagttca atagtagatg
    18241 caataaaatc tacactagaa agaactccac cagaattagc ctcagatata atggaaaacg
    18301 gaataatgct aacaggagga ggagctttgc ttagaggtct tgataagctt attacacaag
    18361 aaactggtat gtctgtacaa atagcagaaa cacctcttga ttgtgttgca cttggaacag
    18421 ggaaatctgt tgaagaccaa gaaatctttg aaaaagtatt aatgatgaat actaagaatt
    18481 agaagttggt gatggccttg agatttgaca agaacaaaaa tgagaaaaaa aggattaatg
    18541 ttaaagtgat agcaacgggc gtagttgcta tcactttaat tggaattgtg ggtatatcaa
    18601 ttggtaaatt ttctagtggt agtccaataa atttgggagt agcttcagat gctattacaa
    18661 gtgtaggtaa gggaataaat gatggatttt cttttatcaa aaatggtttt aaagatgtag
    18721 caaattttaa agataattct aaaaaggtaa aaaaattaga agaagaaaat gaaaaactta
    18781 aaaaagatat gatagcttta aatgcaaaac tagataaaac agaatcgcta gaagagttaa
    18841 aaaagacact gaactttgta caagaagagt ataaggcgac aagtatttca gcaagtgttg
    18901 ttggtaaaaa tgatggaaac tggtatgaga gttttgtcat aggtgcagga aaaaattcag
    18961 gtgtaaaaaa agaaagtata gttatgaatg gtagtgggtt agttggcata gtttatgaag
    19021 tttccaacaa ttatagtaag gcaatttcac tattggattc taaggcatct gtaagcttta
    19081 aactagcaaa ggatgccaat gctaaaggta caattactca aaatactact ttagataata
    19141 aagatagtta taatagtaaa ggatatttgc aaggatatat gtttgactca tcatataatg
    19201 taattcaagg agatataata acaacatcag gtcttggatt cttcccagat ggtataccaa
    19261 ttggcgaagt tgaaaaagtg gttgatgata aagataagtc attaaaatat gtagttgtaa
    19321 aaccttatgt tgattttaag aatataaatg acgttgtagt catagaacca agaaatatag
    19381 gataaagggg ataaaaacaa tgaaaaaagt tttactttgt ctgttgggga ttgtgcttat
    19441 gactatagaa aatagtataa caaattatat agatattttt ggcataagct ttaatttagt
    19501 tcttatatat gtaaccataa tctctcttta tttagatgaa ttagaaggtg ggattatagc
    19561 agcaataata gggcttatta aagacataac cgttggtgga atttttggtg taaatgcatt
    19621 aattttgttt attattgcat atgcaattgg atacatgaga gatcagctat ataaaggtag
    19681 ttatattatt actttcgttt taatttttat aggcagttta attgattcaa ttgtcaatat
    19741 aggtacatct agtataattt atcaaagcca caacatatca acactttttg taaaaggtct
    19801 actagtagca ccaatagtga atagtttaat tggtttagta atatatcgct tatctaaaaa
    19861 agcagtttta aaacttaaag aagattagtt ggtgatttca tggaagaaaa taaacaagtt
    19921 gatagactaa aaataattaa gatagtcatg agcgtgatat ttttagctat tttagtaaaa
    19981 ataatctata tgactacatt taaatatgaa tattacaatg agttagctga gaataaaaca
    20041 tataaaaagt tggcaataga agctccaaga ggagaaataa aagatagata tggaagactg
    20101 ttggcaggaa ataaaaactt atttactgtt caagtttcag gtaacgatat taataaaaaa
    20161 gatgcaaata agcattcaag agcaaatgaa atatcattaa aacttataaa tttgctggag
    20221 agaaatggtg aagaatatgt agatgagttt cctatatatg tagaaaatgg aaagtactat
    20281 tatacatatg acagagatat tagggaatat aaatcagaaa atggtattcc aaatgattat
    20341 aatgctaagg aaagttttta ttatttagta gataaactta tatcagctgg aatattatct
    20401 caagaagata aaagattgga tgcgacaaga ttacaggcaa aactaaatga aaatggatat
    20461 tatccaccta ttttagttag taaatggatg ttcacagcag aaagagataa gagagattgg
    20521 ttggctagtt ataagataaa agaaactaaa ctaagtgcca aagaagcgtt tgaaaaagta
    20581 agaaatagtg atgcattgga aattgataaa agtttaagtg atgaagatgc aagaaaaata
    20641 atggtcgtaa gggatttgat aaaatcaaaa ggatattctc aatataatcc agtaactatt
    20701 gctaaggatg taggagaaac tacgattgct caaattgaag agcgtgctat ggaccttgtt
    20761 ggtgtttcaa tagcagtaga accagtaaga tattatccta atgggtcatt agcttctcat
    20821 atgttaggat atgtaggaaa aatgccatcc actcaaatag aatcatacct tcaaaaaggt
    20881 tatgaaactg gagatatggt aggtcttgca ggagtagaga agagtaatga aagtagatta
    20941 agaggaactg atggttataa aatggttaaa gttgatgcat tgggtagaat cagtaaagaa
    21001 attgaatcta aaaaacctaa atcaggagat actgtgtatc ttacactaga taaagattta
    21061 caagaagttt cagataatgc attaaaacag ataatcgaag ttgcctctaa aggaggtact
    21121 tttaaaagta agtttgggga taaaccaatt agtgcatatg caggaaaagc acaatcagca
    21181 gctcttatag ctattgatgt taaaaatgga gaagtattag cttcatcaag ttatcctaat
    21241 tatgacccaa ataaatttgc aaaaggtatc tctacagagg attataaagc acttcaacca
    21301 aaaaatccaa atgacttact agcaggaagt ccacttttaa acttggttac acaaggggaa
    21361 tttcagccag gttcttcatt taagatggta acttcaatgg cagcgttaga aaatggtctg
    21421 gatccaaact ttactataaa tgaccctgga gttataatgt taggtaaaaa atcttttggt
    21481 gactatgtat ggaaccatgg tagaggaaac catggtatga caaacttata taaagcaata
    21541 caagagtctt gtaatatata tatggcaaca attggtacag gtaaaacatg gccaggtgga
    21601 aaaagcattg gtatagatat gaatgcaaat aaaatacttg agtatgcaaa attatttggg
    21661 cttgaccaaa atacagggct tcaagatgaa gtagaagaaa gagcaggtaa agttcctagc
    21721 acagaagata aattgaaatc aacacaagct cttctaaagt caaatttaga aagagaaatg
    21781 gcaaatgatt ttgttgatat aactagagaa aaaaatccta aagaatatga gaaaagaatc
    21841 aatgagattg taagctgggc agcagaaaag aaaactcctg gtagagtcga aacaatgaat
    21901 aggctaaaga aaatgaatgt taaagaagat agaatagaag atgtagcaga tttagcagta
    21961 ttcagttatt ttaattttgc aaaatggagt actgcagata cattcaactt agctataggt
    22021 caaggtgaga atgcttatac accagctcaa atatcaagat ctgtagctgc tatagcaaat
    22081 ggaggtaatt tagtagaatt atctgtagtt gatagagctg tttcaagtga ttattcatca
    22141 gttaaaatta atgaccagaa gaaagtagaa aaaatacctt ttaaaaatcc agataactta
    22201 aaagaattaa caaaaggtat gaaattggtt gcaagacaag gtacagcgaa gagtgctttt
    22261 gctgattttc caatagatgt agcagcaaaa actggtacag ctgagaaaag tggtaaaata
    22321 cctacagata atgagtatga atatttaaaa agtcatatgt catcttataa tgtcaaccta
    22381 aatgatgcga ttaaactagc tgataagatg aaagcagaaa aagaaaaaga gctttcatta
    22441 gctaaggaaa aggaaataaa aaagaaatta gaaaataaag atttgaaaga cgaagaaaga
    22501 aagaaattag aggaagaatt agaagatgga gtaaaagtta gattagagga cactgataag
    22561 gtgaattctt catatctaag aaaagctata aaagaattaa atccaaaaat aacagatgac
    22621 caaatagata gatttaaaca agattatggt tcttttacat ggactgttgc ttttgctcca
    22681 gcagatgacc cagaaatagc tgttgtatgt gtaattcctc aaggtgattc aagtgtattc
    22741 tcacttttac caactagaga agttattgga acttatatgg gattagagcc aactaatagt
    22801 aaaaatgaca acaagacgaa tgatgttaac aattcttctg acgaaaatat aaatttcgag
    22861 tcacaaatta atagatagct caatctatgt ataaacttta gctttatata gaaaaaataa
    22921 aagggtttaa agcgtatttg aagaatataa atgatatatc actctggagg ttgttatgtc
    22981 tttaagagaa atttgtagtc aagaactagt agagttcaag ggtaataaaa gagggataat
    23041 agtaaatata aaaagagaag ctccatttga ggaaatacag gaaaaaataa taaacaaatt
    23101 ggaagcatat gttggctttt ttaatggggc taaaattagt aaaattaata gtgattgttt
    23161 aactgacatg gagattctag aactgaaaga aggaattact tctagatttg atgtggaatt
    23221 tgttgaagat caaaaaattg aagaaaatag taattttcca acaaaatatg tcaataccct
    23281 aagatctggt gaaaatattg aatttgaagg tgatgtagtt atattgaatg atatgaaacc
    23341 tggttcaaaa gtcctttcaa aatcaaatac agttgttatg ggagatataa atgctggtgc
    23401 taaggttgta gcaggaggta atgtttttgt aatgggtaaa atagaaggtt ttgtacatgc
    23461 aggtgcagaa ggcaatgaat ttgcatatgt tgttgctgga aatcttaatc ctaaaatatt
    23521 acaaatagca gataatattg cagaagctcc agatgatgaa gaaaattatg aaagtgaatc
    23581 agaaattagc ccagaaatag cctttgtaag taatggaaga attgtaatcg aaagctattt
    23641 gtcaaagtta gacaaataga aagaaaaata gggggtagta ttatgagcga agttatagtt
    23701 ataacatctg gtaaaggtgg ggttggaaaa actactaccg cagcaaactt aggaactgcg
    23761 ctaagcctag aaaataaaaa aacagtagtt gtggatgcag acattggact tagaaactta
    23821 gatgtagtaa tgggtcttga aaatcgaata gtttatgaca tagtagatgt agttgaagga
    23881 acttgtaggc ttaagcaagc tctaataaaa gacaaaagat ttgataatct atatttatta
    23941 ccagcagcac aaactagaga taaaaatgct gtctcagttg agcagatgat tgacctgtgt
    24001 gagaaactaa aagaatcttt cgaatatata ataatagact gtccagcagg tattgagcaa
    24061 ggttttaaaa atgcagtagc tggagcagat agagctattg tagttactaa tccagaaata
    24121 tcagcagtaa gagatgctga tagaataata ggtctattag aagcaaatga aataaaagag
    24181 ataagattag ttataaatag aattagaaat gatatggtta agcgtggaga catgatggat
    24241 aaacaagata taatagaaat attagcaata gatttgttag gtcttgttcc tgatgatgaa
    24301 agcataatta tatcaacaaa taaaggagag ccggctatac ttgattctaa gtcacttgct
    24361 ggtcaagcat acaaaaatat cgccaaaagg atactaaatg aagaagttcc tctacttgat
    24421 cttgaagttg aagatggatt ctttggtaga cttaaaaaaa tgtttagcat ggctaagtag
    24481 caaaggagga tacatctgtg ttagatttat ttagagtttt ttctaacgag gctaaaacta
    24541 gtaagtctgt tgctaaagag agattaaagc tagttttagt tcatgataga gtagattgtt
    24601 caccacaact tttagagatg ataaaaacag acattttaaa agtaatagct aattatgcag
    24661 aaatagaaga tgatggcctt gaaataaaaa tgtctaaatg cagaggtgag catgatgaca
    24721 aacctgtatc tgcattagtt gctaatatac cactaaagaa tataaaagat agatgtatgt
    24781 aattagtgct taaaaggggg atatccttat tggtatatgc cccttttatt taattatatg
    24841 tttaaagata gaaagacggt gattctttga aaaaaatcaa tattaattat aagtcgacaa
    24901 ttaagttgat aaaacagtta gactggaaac ttatagttac agtattagct atctttattt
    24961 ttgggcttgt tatacttagt agtgcaacac atgctaattc aacaggttca tataatcaat
    25021 tgattaaaca agggctagct tttgtacttg gtataggaat gattatagtc atattatttt
    25081 ttgattataa tcttctaggc aggtattata aagcacttta tataataagt cttgttttgt
    25141 tagccatcgt tttattacct ggaattggga cagtaaaagg tggagctagg tcgtggatta
    25201 acttagggcc acttgacttg cagacatctg aaatagtcaa attaacattt gtactaagtt
    25261 atgcaaagat acttgaatcc aaaaaggata aattaaatac attaaaggaa gttatgcctg
    25321 ttgtagttta ttctcttcca tttatcggat tattgattgc acagccagac ttgggaacag
    25381 gaattgtatt ttgttgtatg atatttgcaa tgctgttcac agcaggtctt agttcaaagt
    25441 tgataaaaag aggaataatc atactattgg tgtcaatgcc actgatgtat ttaatgatgg
    25501 cagaccatca aaagataaga atagaagctt ttctaaatcc agaagatgta acactaaaag
    25561 gaaactatca agttatgcaa tctctgattg caattggttc tggaggtgta actggtaaag
    25621 gactttataa tggaagtcaa aatcaagaag attttttacc tgtacaagat agtgacttta
    25681 tatttgctgt tgtaggtgaa gaacttggtg taataggaat ggcagtgtta ataatcttat
    25741 ttatgatatt cctattaagg cttttagcaa ttgcaagaga tgcaaaagat ttttatggaa
    25801 ccctcatcgt ggttggtgtt atgggaatgt ttggttacca gataatccaa aatataggta
    25861 tgacagttgc gttaatacca gtaactggag taacattacc ttttgtaagt tatggaggta
    25921 gttcgttatt gacatcttta gcaaacttag gattagtact aaatgtgtgt atgagaagaa
    25981 agaaaataaa tttctaaatt taaatacatc taaacataaa ttgaggagat tagatatatg
    26041 aatatagcgt tagtagcaca tgaccaaatg aaaaacacta tggtcggatt ttgtataggt
    26101 tatgaatcaa ttttaaaaaa gtatggacta tatgcaactg gaactacagg aaaaagaata
    26161 atggatgaaa ctgaattaaa tataaacaga ttagcatctg gtcctttagg tggagaccaa
    26221 caaataggct ctttaatagt aacacaagag atagatttag ttattttttt aagagaccca
    26281 ttgacatctc aggctcatga aacagatata caagctttaa taagactttg tgatgtttat
    26341 catgttccaa tagctacaaa tctagcttca gcagaaatat ttataaaagc tcttgataga
    26401 ggagaactat catggagaga ggtcagaaaa agtaaaagtc aacgtgttta aaaaagtaaa
    26461 tagtatacaa aaattaggag atgtatcaaa ataaaaattt gaatcatctc ctttttatat
    26521 aaaaatagaa atgatatttc tagaaattaa taacctattc gataattatt cagaaaaagt
    26581 actgtatatc actgcttttt ctcatttgaa atatatgtat agatagtcta ataccagtag
    26641 actatctgtt tctttatatt ataaatataa ttgagatttc taatgtaact atactttcca
    26701 aaaaatgtat tgaatataca aattataatc tgtataatgt tgttagggga gaatgtatga
    26761 agtgtataca actttaattt taaatgtatt attgacaatt ctcctcactc tcgccattac
    26821 taataattgg aaaattgaca tatctgttac agaccaacat tttcgaatat agtaattatt
    26881 atcattttat tgtgatgata tcaaaaatgc aactttgcga gttggattgt atacgaacta
    26941 tataggaaat atagaacaga ttttccaaga aatctaaata ttctatgact acgtgaattg
    27001 ctatgcacac atgttgataa tccacatttt aaatctgaaa tgaacttaat taatggtagg
    27061 gaattgaaag taaaaatata ttataaagga gaacgctaat atgaaaaaaa tcaggaaatt
    27121 agctactaaa ctattaattg caacgatggt tttaacctca tgtatgagtg taacagttta
    27181 tggtatgaca gctaaagacg ttactgcaaa gacaccgaaa agctatgtag ctggaactaa
    27241 cagtatatat ggtccaaagt tgtcacaagc tcaacttaat tcggtagcac aagcaacagc
    27301 agactttatg aacaagaaaa ttacgaagaa catgaccaca gatgctaaga ttatggttgc
    27361 ttacaatcac attaaaaata acacaacata tgttgactgg aatgcagcag agggagccaa
    27421 tactgcctat acattggtta ctaaaaaagg tgcgtgctct ggaatgtgta gaagtatgaa
    27481 agctttatgt gatgctatgg gaattgaatg ttattatgta catagtatag caaatgacca
    27541 ccagtggaac cttattcgtt ttggtgatgg acgtttgtat catgtagaca ttgatgccaa
    27601 caaatcagca ggaaaggaca ttatatacaa gagtcttgct cacccattac catttgacaa
    27661 gaacgcatat ccagctgttg gagcagtaaa ggtgcaaaaa ccagcatcac ctgggaagca
    27721 cactatgcta gcagatcaag aaggggcaat acttaatgaa gtttatggaa ataatatgaa
    27781 gccacaaaat aaaatgtatg agggggttcc atgtagttca attgactcat caataaatac
    27841 aattaagttt acaagtagta attcaaattt agacccatca aagcatacga tttctgtaat
    27901 aggatttatg aaaacaccat atggtatggt tgcattagca ccagatagta atcctgttag
    27961 ttatcctttt gaatatgata agccattttc tttagatttt actggtaaga accgttctgc
    28021 agcagaattg tcaaaatact gtaaaaaaaa taatataatt attaaagtgg aaattggtga
    28081 aacagcatct aattctggaa gaggtatggt agcatacgct agatacgtga attaatacaa
    28141 atttaataaa tttataaaca atgcaccctc ttgttgaggg tgcatttaca ttatatcagt
    28201 tattttaaat aatagtatgt gtgattaatg actatttaac taattttgca aattctgcac
    28261 caaatgccat acactctttt aatgtctctt cactagggaa gaattttttc ttaagagatt
    28321 ctacaggcat tttgaatgac atagatttaa gtaaagtctc agccatagtt ataccttcac
    28381 cactccaacc aaatgaacca aatactcctg ctatcttgcc ttggttagcc attgggtcta
    28441 taacagagaa taaatcccac ataggtttaa ccatagtttt gtttatagtt ggagaaccta
    28501 ataatattac ttttgatact actaatgtat cgtgcatttc tgttaatgtc atatcttcta
    28561 aatcatataa ttcagccttt gcaccttctt tatcaagtcc ttcttttatc tttttagcca
    28621 tctctaaagt attactataa gctgataagt agaatataga aacttgattt tgattagttg
    28681 tattaacagc ttctgtagac cactcaacat atcttttaac agcagccatt gggtctttag
    28741 taagcattgg accatgagaa gtcaatatag tatcaaattc tatgtttagt cctaccacct
    28801 tatctacagc acttaaaaca tgtttagcaa atggcttaac tatacaatca tagtagtgtt
    28861 tagctgattt tagataatct tcactattca ctacctctgc atcaacactt gcaaagtgac
    28921 aaccaaatgc atcacaagtt aaaagagtct tgtcctcctc aatgtaagta aacattgtgt
    28981 caacccaatg taagaatgga gcagttataa atctaagatt tcttttaccg atatttaaaa
    29041 tttcaccatc ttttattaca tgacagttaa aaggtctatt tatttgacca tctaagtata
    29101 gtttagcagc tttagtacaa taaacttcta tattaggatt tatatctaat aagtatttta
    29161 aactacctgc atggtctggt tctgtgtgat gaattacaac atagtcaatt ttagcgatat
    29221 cagtaacctc tgataaatta cttaaaaact catctttaaa atttgccttt acagtgtcaa
    29281 ataaaaccgt tttttcgtcc ttgattaaat aagagttgta agttgtccca aactcagttt
    29341 ccattattat gtcgaatact tttaaacctt catctactac gccagtaaag tagatatctt
    29401 tttttacttc aaaaacttta ctcattttta accctcctaa aatttatcat tatataagta
    29461 acatacccca ttttgaggtg aatgaaacaa aagatgtcat gataagtcat gtaaaacaat
    29521 agttttatgt ttataatata tcatactttg taaaaaaatt gtatttcaat aaaaatttaa
    29581 tagaaaatat tcttaaatat ttttagctat tctattttgt gaataatatg tattttaaat
    29641 tcaatatttt agttatattt ttgatagaaa atattttatc gtgtataata ttacttataa
    29701 ataagaataa aaataaagag cttgtttgga ggaattttaa taaatgaata aagtagactt
    29761 gaaaaaaatc ttaaaaaaag tagaaaagcc tgctagatat tttggaaatg aaataaattc
    29821 aattcataaa gatactagta atagtgaatt gataagatat gcacattgct ttccagattt
    29881 atatgaagtt ggtatgagtc acttagggag tcatatatta tatgatgtaa taaataagga
    29941 tgaagatgta ttttgtgaga gagtgtattc tcctgcagtt gacatggaaa atatgatgag
    30001 agaaaaaagt ataccattat ttgcattaga gtcaagagag cctataacaa actttgattt
    30061 tgtcactttt acacttcaat atgaactttc atacactaat atcttaaata tattagattt
    30121 agccaatata ccaatattaa aagaagaaag aactttggaa gacccattta taatggttgg
    30181 tggtccttgt gcttataatt ccgaaccatt agcagatttt gtagatgtag taatattagg
    30241 tgaaggtgaa gaggttaatt tagaagtagt aaatgagtat aaagaatgga agaaaaataa
    30301 aacgactaga gaagattttt tatataaaat atctagcata gaaggagtat acataccaag
    30361 tttttatgat gtcaagtaca atgaagatgg tacagttcaa agtgtaactc ctaacaggga
    30421 agggattact aaaaatccta ctaaaaggat aataaaagat gttgagactg tagattatcc
    30481 agaaaaacta atagtacctt atatagatac tgttcatgat agaatagtac tagaattatt
    30541 tagaggatgt actagagggt gtagattctg tcaagcaggt atgatataca gaccaataag
    30601 agaaaagagt gttaaaagac ttaaagaaat attagataaa ttagttaaga atactggata
    30661 tgatgaaata tcattatcat cacttagtac gagtgattat agtaagttat cagagttgac
    30721 tgattatttg gtagatgaat atgcatctaa taatattggg atttcgcttc catcccttag
    30781 attagataat ttttcaatgg aaattgctga taaaatacaa caagtaagaa aatctgggct
    30841 tacttttgct ccagaagcag gaactcaaag acttagagat gttataaata aaggtgttac
    30901 agaagaagat ttggaaaatg caacagaaag agcttttgag atggggtgga atagtgttaa
    30961 gttgtatttt atgattggtt taccaactga gacttatgaa gatttagatg gtatagctaa
    31021 attagcttat aaagttattg atatttatag aaaagtaaat ggaggaaagc taaagagaag
    31081 ttttagtgtt acagtgagta cttctacatt tgtaccaaag ccatttacac ctttccaatg
    31141 gcatggtcaa gatactacag aagaagttat taataagcag agacatttag taaataaatt
    31201 aagaaacaac aatataaaat acaattacca tgattcaaag acgagtttaa tggaagcagt
    31261 agtagctaga ggagatagaa gaataggaaa agtgatatat gatgcattta gattaggtgc
    31321 aaaatttgat ggatgggcag aacactttaa tttagatata tggaaagaag ctatggaaaa
    31381 gaataatctt tctatagatt tttatgcaca tagaaataga aactatgaag aggtatttcc
    31441 ttgggaccat atagatgtag gtattagtaa aaaattctta atcagagaag acgaaaatgc
    31501 taaaaaagaa aaaatcactt ctgattgtag acataactgc aatggctgtg gaataaatat
    31561 acatgatata ggaagggggc tttgctaata tgagtaagat aataagagta aaatttaaaa
    31621 aggaaggcga catgatatat atatcacatc ttgaccttca gagattgtta caaagagcat
    31681 ttagaagagc tgaaataaac ttatcacatt ctcaaggatt taatcctcac cctaaaatga
    31741 gttatggcaa tgctttggct ttagggacag aaagtcaagg tgagtatgta gatattgaaa
    31801 tagaagaaga tgatttatca gtagaggaat ttttaaataa agtatcaatt caacttccaa
    31861 atgggataga ttttatcaaa gcaaaagaaa ttgatagaaa aacaccttct ctttcttctg
    31921 tgatagacta tggagaatat ttgtttaata tagatttgaa aagacctatg acgaaagagt
    31981 ttgtaaaaag aaaagtaatt gactttatga ataataaaga aataatcata acaaaaaaga
    32041 ataaaaaagg taagatggtt gaagtagata taagacctat gataagaacc tttgatgttt
    32101 taaatttaga agatgaacat attactctta ctgctactat agctacaggc tcaaaaacta
    32161 atttaaatac taatatactt ataccaaaga tattagagat gtttgaactt gatattgacc
    32221 cattagatgt tgatatactg agaagagatt tatatgtatt agaagatggg gaattagtga
    32281 ctcctatgta agctcgagag gtgaagttgt gaaaaagata gttattgagt ccctaatagg
    32341 gtctcagaaa acagcagtac ttgaagatga aagattaaca gaactttttg ttgaagacaa
    32401 tttaaataag aagactgtat ccaatatata tcgtggaatc gtaaaaaaag ttataccagg
    32461 aatagaagct tgttttgtag atattggatt taagaaactt gcttatttac aattaaaaaa
    32521 gggaagtact ataaaatctg gtcaggatat tttggttcag ataaataaag aagaaatagg
    32581 aactaaaggt gcaaaattaa acactgagat tagtatttct ggaaggtata ttgtctatat
    32641 accttccaat gatagaacta caatatcaaa taaaattact gatgaaaaag aacgatttag
    32701 attaaaaaaa attactaagg cagtaaataa ggaaaatcta ggattaataa taagaactga
    32761 ggctcaaggt tgtactcatg atgaaataaa gaaagatata gaagagttaa aattaaagta
    32821 tgaaaacatt ttaaaagaat ataaacttgg tataggcccg aaattattat ataagtcttt
    32881 agattttgca actaaatacg taaaagataa tgtgaatgat gatattgaat ctataattac
    32941 caatagttat gataagtact ctgaacttaa gtctatttta agaagtatag ataagactta
    33001 tgttgataaa ctatgtcttg aggaaaatag agatgtattt gatttataca gaatagaatc
    33061 taaaatagaa aaattattaa ataaaaaagt atggttaaag agtggaggtt atttaataat
    33121 tgaaaaaaca gaagctctta cggttataga tgtaaatacg ggtaagttta ttggaactgg
    33181 taaacttgat gaaaccgttt ataaaactaa tcttgaagct gcaaaagaaa ttgtaagaca
    33241 gcttagaatt agagacatag ctgggataat aataatcgat tttattgata tgcataaaaa
    33301 gaagcatcaa aaggagattt tacacatcct agaagaagaa tttaataaag ataaaagaaa
    33361 agctgaagta ttaggtatga caaaacttgg tttagtagaa gttgctaggc gtagagaaaa
    33421 agaatccatt gataagtatt atttaatgtc ttgtccatgt tgtgatggtg aacaaacaat
    33481 aaaatctgta cattatatac tagatagtat tgaaaaagaa attatgagaa taagtgaaca
    33541 tactgtttac aaaaacataa tggtagaatt taatgatttt atttttgaac aaataaaagg
    33601 atattatatg gatataattg ataaaattgg tgaaaaatat aatataaaga tttctttaaa
    33661 cgcaaatagt acattaaagc acaataaaac caatgttatt ttcgataaaa tagttgacaa
    33721 taaaatgtaa atattttata attaataatt gtaatgccgc acggtaaagg tttaaatctt
    33781 caaatgaagt accttatact ggcgagtttg aggccgagga ggtgtattta atgtacgcta
    33841 tagtaaagac tggtggaaag caatataaag tttctgaagg tgatgtatta ttcgttgaaa
    33901 aattagaagc taacgcaggt gatgttgtta ctttaaacga agtattagct tgctctaaag
    33961 atggagaatt aaaattaggt tctccagtag tagaaggagc atctgttcaa gctaaagttg
    34021 ttgaacaagg taaagctaag aaagttatag ttttcaagta taaagcaaaa aaagattaca
    34081 gaagaaaaca aggtcatcgt caatcataca ctaagatagt tgtagaaaaa ataaacgctt
    34141 agtaattggt taagttatga taaaggttaa atattattat aatgatgatt tcttgttgca
    34201 aggtttttgt ttaaagggac atgctgattt tgctgaaata ggctatgata tagtatgtgc
    34261 tgcagttaca tctaatgcta ttgcagttat aaattcttta gacaaattac aaaagattga
    34321 atttgaaaaa gtagtaggag aagaaggtca tatagagtgt atagttaaag atgcacatat
    34381 aaaagacgca caattattac taaatcactt tcaattagcg gttgaagaaa ttaaacggga
    34441 atacccaaaa aatataaaaa ttttaaaaaa gtagggggtg actccatatg ttaaacatga
    34501 atttacaatt attagcatct aaaaaagggg taggttcttc taaaaatggt agagattcta
    34561 tatctaaaag attaggtgtt aagagattcg atggtcaatt agtaactgct ggaagtataa
    34621 tagttagaca aagaggaact aaaatacatc caggaactaa tgttggaaaa ggtagcgatg
    34681 atactttatt tgcattagtt gatggtactg ttaaattcga aagaaaagac aagaagagaa
    34741 aaaaagttag tatatatcca gtagcaatag ctgagtaata tacataaaag gagtgttgag
    34801 gcactccttt ttcttataat actgatgaat ttataatggt atttttggta atgctaatta
    34861 aaagttttta attaagtagg tgaagaattt gtttatagat aaggcaagaa tatttgttaa
    34921 ggctggaaat ggtggaaatg gttcggtagc atttagaaga gaaaaatatg ttccagctgg
    34981 tggtccagac ggtggagatg gtggtcgtgg agcaagcatc atatttgaag ttgatttggg
    35041 gcttagaact ctaatggact ttaagtacca gaaaaaatat caagctcaaa atggtggcga
    35101 cggaagcaaa ggaaaaagag ctggtaaaaa tggagaaaat ttagtattaa aagtaccagc
    35161 aggtacagta ataagagatg aggctacagg tcttgtacta gcagatttaa aaaaagaagg
    35221 agatacagct atagttgcaa aaggtggtat aggtggtaaa ggtaatcaac actttgcaaa
    35281 tgcagtaaga caagctccag cttttgcaaa atctggtaca gatggagaag aaaggtggat
    35341 aactcttgaa cttaaaatga tagcagatgt cggtctttta ggatttccaa atgtaggtaa
    35401 atcaacattt ctatcagttg taactaaagc taaacctaaa attgcaaact atcattttac
    35461 aacacttaca ccaaatttag gtgttgttca aactaagttt ggagatagtt ttgtacttgc
    35521 ggatatacct ggaattatag agggtgcttc tgaaggtgta ggattaggtc atgagttctt
    35581 aagacatgtt gagagaacaa aggtattgat acatattgta gatatatctg gattagaggg
    35641 aagagaccct attgaagatt ttgacaagat aaatgatgag cttaaattgt acaatgaaaa
    35701 actttcaaag agaccacaag ttgtagttgc aaataaattt gatatattag aagatgagag
    35761 taagtttgag aagtttaaga gtgaattaga aggcagagga tatacagtat ttaaaatgtc
    35821 agcagcaact cgtcaaggca tagatgaagt aatagcctat gtttctaaaa tgctaaaaga
    35881 agtagaagat gtggagctag tttcagaaga agaaatgtat agaccagaac ttgatattgg
    35941 tactgaagaa gagttaagta tagatattga agatggtgtg tatgtagtta caggtaaagc
    36001 acttagaaga ataatgtatt ctgtaaattt tgatgatatg gaatcccttc aatatttcca
    36061 aaaagctatg gagagtcaag gtgtttttga taggcttaga gaaatgggaa tagaagatgg
    36121 agatgtggtt aaaatttatg aattagaatt tgagttttac aattaattta aatatagatt
    36181 acagataatt taagtataga attatagagg tgagaatatg ttaaaaggaa aacaaagggc
    36241 gtatttaaga tctttggcaa atactttaaa gcctactaca caaattggga aagaaggtgt
    36301 tacagagagt ttcttagagc aattagatgg tatgttaaga actagagaat tagtaaaagt
    36361 tactatatta gaaaatgcag gtcttgatac taaagaaact gctaatgcag tatgtgaagc
    36421 tttaagagct gaatttgtgc aggctattgg ttttaaattt actatttaca agaggaatat
    36481 tgaagagcct aaaatacttt ttccaggaca tgaacaggcg aaagcaaaga ctaaaaataa
    36541 taatgttaca aaaaagggta aaccaactaa aagagctgtt agataatagt ttatatcctt
    36601 ttagggaaat aagttaaaaa aatagtgaca attggaagaa attgtattat actattaaac
    36661 ataaatttaa attagcaatc ttatcaagag tggcagaggg actggccctg tgaagcccgg
    36721 caaccagtat atttaattat acattggtgc taaatcctgc agcatttatg ctgaaagatg
    36781 agtataaacc cgtcatatgg cttgagttta tactcaagct ttttttattg gtatatttat
    36841 atatataaat aatttaatat aatcaaaatg atgggggtat gttacagtga agatagcaat
    36901 attagggtat ggaacagtag gaagtggatt aattgattta atagaaaata atacttcaaa
    36961 aagagatata gaagttgttg gaattttagt taaaaataaa gaaaaacaca agtataagaa
    37021 gtattttgat aagataacta ctgatataga agatatattt gacaaggata ttgatatact
    37081 agttgaagta attggaggat taaatccagc ttttgattat gtaacaagag cgttaaataa
    37141 aaaaatacat gtagtgacag ctaataaaga tttactggct gaaaaaggta gtgaccttat
    37201 agaattagct aacttaaata atgttagcat caagtttgaa gcatctgtgg caggtggtat
    37261 tccagtttta aaaccattga tagaatcact ggaaggcaac aatataaaga gcataaacgc
    37321 tattttaaat gggacttgta attttatatt atcaaagatg tatgatgaaa atttaccgta
    37381 tgatgtcgct ttaaaacagg cacaagagtt gggatttgca gaagttaatc cagatgcaga
    37441 tgttttagga tatgatagtg ctagaaagtt atctatattg tctactttat cttatggaaa
    37501 aatagtatat tggaaagatt tattattaga aggaataact tcaatagatg aaaaagatat
    37561 agaatatgca aaaaaattaa attgtaagat aaaattggtt gctagaagta agtatgaatc
    37621 aggagaagta agtggatttg ttagaccagc attagtggat aataataata tgctttcaaa
    37681 gatagataat gagttcaacg tagttatttt agtaggtgat tcagtaggtg aactttcctt
    37741 tgtaggcaag ggagctggaa gaggtgcaac aggaagtgct gtttatgcag atattataga
    37801 tataatagat aatagaaatt caaatataaa atctttttca aaaggaaaat tagatttaag
    37861 tggacttata gaagatgagt gtagtgcagt agttagattt ggtgggtata ataataaaga
    37921 tggaatttta gaatgtttag ataaatatgt agatagttat gatattatag atgatgaaga
    37981 attggcgatt tttgtaagtg caaagtcaga acatgagatt gatagatttt tggaagttgt
    38041 aaaaagaaat aattatagtg aatcagctaa aaaattattg aagatagatt aatatgttta
    38101 tgcaagagtt atgaaaattt aatagggtgt ctcaaaatag atagataagc cttttttcca
    38161 gtattgaaat aataaaaata tatttgattt tataaaaaaa gagtgtctca tgaactaaaa
    38221 agttcatttt gagacactct tttttaatat ctggtgaatt aataagataa tagaaaaatg
    38281 tatacgttat aattaaccaa taaagaaatt ttcactagct tcattaatag cttgctgagt
    38341 tacccttata tatcgctgtg tagtggctac agagctgtga tttaaaagtt ctttaaccaa
    38401 ctctaaatta ttgttactct tttcaaattg agtagtagca tagagctttc taaagctatg
    38461 tgtacttata ttatacagat ttaagtgttt acaaattatc cttaactgtt tttgaatagc
    38521 ctttgtcttt atatttacta agtagtcatt tgatttaaga ttatactttc ttgcatgttc
    38581 atttatggac tcaattatat ttttattaac tggtctatac tgtaatttgc ccgtcttttt
    38641 ttcatttatt tccaacatat tacctttaaa attacctatc tttaatctta aaatatctga
    38701 tattcttaga cctaaattag cttctaacat tagtgccaat gcaactttag gattttttct
    38761 aaatcttttt tcaacgccat tttcactata agtaaagccc gtatgtaata gttccattat
    38821 ctttttatat tcgtctattt ctataggtct tgttgccatt ttcaaccact cctcttagtt
    38881 tgatttggta agtttaggtc ggtatttatt atataatata aatgaaatat tatataatgg
    38941 aaaaatttac cagtaaactt atacaagtgt gatatatgtg attaaggaga attttttgaa
    39001 aaatatttct aataaaatta ttaaattgtg atgaaataac tatgctgtta aactaattaa
    39061 aaaggttatt aaaattatga caaatataat aaaaaaatgg aagtatttag ttaagtttta
    39121 ttatttttat ttttttcgtg aaaatttctt aatgaaaaaa gtatttttat atcgcattag
    39181 tttcccattt gtagataaag ttttcatatg tttttttgca ataaatacgg ctgagattag
    39241 ataaattatt actacaattt gaagaattgt atcataatag ctaagataat aaaaccagat
    39301 taaattatta tatataacgt tgtatgtatc agcaaagcta gaaaccaaca cagcagagtt
    39361 tgcaattgga taaagtataa atataaagat agcagctaaa actccatgcg caaatttact
    39421 tagtatgtat aattttaaat ctatgtccgt ttttgataga aatgtacaac attgagctag
    39481 gatggataat ccactaaatc ctatcaaaaa gctagctaaa gatgctctta aaatttctgg
    39541 agaacttgtt atactagata ctttattgca tcctatagta atttcaaata gaccgcttac
    39601 aaatgcttga cagagttctt tactaacacc taaaaatgaa agaggcaaat atataactga
    39661 tgctattaat gagattacat taaaaagaga tagtatttta aatactactg aaaaaacaat
    39721 tacaaatcca ccaactgtaa gtaatgtatt tactccatta aatacagcat ttccaaataa
    39781 aacaaaaaaa ccattcccac ttgaatttct aatgttgatt acatttttta tatttgtttt
    39841 taaatccaat tttgtctttg gaagattttc attaccataa tttctaaaga aaagaccaac
    39901 caatatagtt cctagataat ggcagagaat cattaaataa cctaagtttg aattttggaa
    39961 catacctgta ccaactgaac ctattatgaa taaaggtcct gatgtagaac aaaaagacac
    40021 aagtctttga gcttcatgct tagaaatttg tccattactc ctaaattcag aagccaaact
    40081 tactcctact ggataaccag atactgttga tatagcaaaa gctaaagcac tctttcctga
    40141 aacattaaac acaagtctag ttatgggatt gatgataaac cctataatat ccactatttt
    40201 taaatcgaca attaagtttg ctccaattat aaatggtagt agtgatggaa ctaaaacatt
    40261 agaccatata gagaagccct ctcttgcaga ttttatagct tcatttgggt ataggataat
    40321 acctaaaact agaactccaa ctatgatagc tggagcaaaa aaagcaataa ttttattttt
    40381 tttcataata tcacctataa atttgagtta taagatatta tattaaaagt aataaaaata
    40441 tatgaatgta atggcttaag atatataata gatatatatt acaacaacga ttatcatatt
    40501 ggaggaaaaa atgtttaaca gtatagctag taaaaaagtt tatgaacaag taattgagca
    40561 aatacaatat aaaatactaa atggagagtt aaaaaagggg gataaattac tatcagaaag
    40621 agaattgtct gaacaaatga atgtgagtag aacttccatt cgagaagcta ttagggtatt
    40681 agagactatg ggagtgatag aaagcagaca gggtgaagga aattttatat gtacaaatat
    40741 agaaaaaact ttaatagaac ctttatctat gatttttaaa cttaataatg gtacacttga
    40801 agatatatta gagcttagaa taatcctaga aattgagatt gcaaaacttg cttcaaagag
    40861 aataacttct tctgaaatta tagaattgaa acatataata gatgaaatga gagtagaaac
    40921 taataaaaaa gataataatc gagtactggt tttattagac caaaaatttc acaataaact
    40981 agcaacatta agtaaaaatt atttaattca aagtcttttt atgacagcat caaaattatt
    41041 tgatggattt atagaagatg caagagaaaa aataatcgca gaacctttta atgaaaatat
    41101 attgcttaaa cagcatgaag ctatatacaa tgcagttgta gaaaatgatg tagagcttgc
    41161 atgtgagaaa gcaaaggaac atatggattt tataagtaaa aattatagaa aaaatgaaaa
    41221 ttaaaagttt gttataaaat aaattattct tgcaaatggt agataatggt actatcatat
    41281 aaatgtaaaa ctggtatgac cacataccaa ttttatagtt tattataatg ggagattaaa
    41341 aatggtaaag ttaaaagtac cgtattcaaa acaaggaatg attttagaaa tacctgatga
    41401 acgttttctc ggtgttttag agtcaaaatc ggataattat atagaacata agactcaaga
    41461 agagatagtt gaagaatcta tgaataatcc tataggctct aattctctgg aagaactggt
    41521 taaggggaaa aagaatgtag tcataattac aagtgaccat acaagacctg taccaagtag
    41581 aataactatg ccaattatat taagaaggat taggaatgtt aatccagaaa ttgatgtgaa
    41641 gattatagtg gcaactggat ttcataggcc aagtacaaga gaagagctaa tttataaaat
    41701 gggagaagaa atagtagata atgaagatat agttatgcat atatctacgg atgataaatc
    41761 tatgtgtaaa gtagggatat taccttcagg tggagattta tatataaaca aactagcata
    41821 tgaggcagaa cttttaatag cagaaggttt tatagaacct catttctttg caggtttttc
    41881 aggtggcaga aaaagcgtac ttccaggaat cgcatctgca aagactataa tgtacaatca
    41941 ctgtagtgac tttatagata gtgataattc aagaactggg aaattgaata ataatcctat
    42001 ccatgaggat atggtatatg cagctaaagt tgcaaaatta gcattcatat tgaatgttgt
    42061 aatagataaa gataaaaaaa taattgcttc ctttgctggt gatgtcgaaa aagcacatac
    42121 taaaggatgt agatttgtaa cagcgttatc aaaagtaaat tctataaaat ctgatatcgt
    42181 tgtaactaca aatggtggat atccactaga ccaaaatatt tatcaatctg ttaaagggat
    42241 gacagcagct gaagctacct gtaaagacgg tggagtaata ataatgattg cagcatgtaa
    42301 tgatggtcat ggtggtcaat ctttttatga aaatgtagca aatgcagatt ctccaaaaca
    42361 acttttagat aaaattagaa gtgtcgatag agtagatacg attccagacc aatgggaatt
    42421 tcagatacta gcgagaatat tatctaaata tactgtaata atggtaacgg ataagtgtaa
    42481 tcctgaaatg ataaaaaaaa tgcatatgaa acatgcattt aattttaaag aagctttaga
    42541 aatggcaact agtatagttg gtaagaagag caaagtagta gttatacctg atggagtatc
    42601 agttatagtt aaataaaaaa ggagtgtact ttatggaaat tttgacatgt attaaacaag
    42661 ttcctggaac gacttcagta gaagttgatg aaactacagg agtactaaaa agagatggag
    42721 tagattcaaa aatgaatcca tatgacctat atgctttaga aacagctctt agaataaagg
    42781 aagagaagaa agcgaatcta aaagtactta gcatgggtcc tcctcaggca aagaaggtaa
    42841 ttgaagaaag ttttatgatg ggagctgatg aaggcgcatt aataagtgat aggagatttg
    42901 gaggagcaga tgtattggct acttcctata ccatatcaca aggtattaag aagatgggaa
    42961 aggtagacct aataatttgt ggaaaacaaa ctactgatgg tgatacagca caggtgggac
    43021 ctgaagtagc tgaattttta gatatacctc atgttactaa tgttacaaaa ttaattgaag
    43081 ttagagatga aagtatagtt gttgaaatag atatgccaaa tgatttacaa gtatgtgaga
    43141 ttgaatatcc atgtttaata actgtagaaa aagatatttt tcaaccaaga ctaccttcat
    43201 ttaaattaaa acttaatact aaagatagag aaatacctgt atatagctta gatgactttg
    43261 aagataaaga tgaaaataac tatggactta atggttctcc tactcaagtt gtaagaatat
    43321 ttcctccaaa gccaaataca gataaaaata ttgtaagagg aagtgcagat gaactaagtt
    43381 ttgcacttgt aaataaatta gaggaattaa agttggtgta agggggagta ataaatgtca
    43441 aagatagttg taaatcaaga taaaataact gacttaaaga gaattttaga aatatgtcct
    43501 tttggggcta tagaagaaaa gtcaggtata gtagaaataa gtgctggctg taagatgtgc
    43561 aagttgtgtg tcaaaagtgg accaaaaggc gcatttgaat ttatagagag tagtaaagta
    43621 caaattaata aagatgaatg gaggggaatt gcagtatatg ttgaacatca taatggaaat
    43681 atacatcctg taacgtacga gcttataggt aaagctcgtg agatggcatc aaaaataaaa
    43741 caacctgtgt attgtgtatt tgtaggaaaa gacataaaag ataagtgcag taatttgatt
    43801 tcttatgggg tggatgaagt atttgtatat gatgaagatg aatttaaaga ttttaggata
    43861 gaaccatatt caaaagcaat agaaaacttt ataaataaaa taaaaccaac gatagtgtta
    43921 gttggtggaa ctacattagg taggtcatta gctccaagac ttgctgctag atttagaacg
    43981 ggtcttacag cagattgtac aatacttgat attcaatcaa atactgattt agaccagata
    44041 agaccagcat ttggtggaaa tataatggct catataaata cacctaataa tagaccacaa
    44101 tttgccactg taagatataa aatattttca gtaccagaaa aaatagagaa tactacagga
    44161 aagataacct tatgtaaagt agaaaaaaaa gaactaaaat ctaaaattaa ggtactgagt
    44221 gtaaaagaaa aaaataaaga agttggtctg gaagaagctg aagtgatagt tgtggcaagc
    44281 agagcaataa aaaaacaaga agatatggag atgatatata aattagctga taagttaaat
    44341 gctcaagtgg caggaacaag acctgtaata gaagcaggtt ggatagatgc taaaaaacaa
    44401 ataggtctta gtggtagaac tgtaaaacct aaattgataa taacttgtgg tgtatctgga
    44461 gcagttcaat ttgttgcagg aatgcagggt gctgactata tagtagcaat aaataaagat
    44521 gataaagcac ctatacttga tgtagcacat ctagctttaa taggtgatat ttatgatatt
    44581 attcctaaat tgattgaaaa aatagaaaat aacaaagata ataatcaaaa atatatggca
    44641 tcagcagcta aataggggga tagaaatgta taaattagtc gataaaaaag atatagattt
    44701 tttaatagat acttgtggag aagaaaatgt attagttgga agtgatataa atgaagattt
    44761 ttcacatgat gaacttggag ggattgagaa gtatcctgaa gttttagtaa atgtacttga
    44821 aactgaacaa gtatctaaaa taatgaaata cgcatataaa aataatatac cagtcacacc
    44881 aagaggtcaa ggtactggtc ttgtaggtgc tgctgttgcc ataaatggtg gaataatgat
    44941 taatctatgt aaaatgaata aaatacttga agttgattat gaaaatttaa cattgacagt
    45001 agagccaggt gttttgttga tgacaatagg tcaatatgtt caagatagag atttatttta
    45061 cccacctgac ccaggtgaaa aaagtgctac aattgcaggt aatataaaca ctaatgcagg
    45121 tggtatgagg gcagtaaaat atggcgttac aagagattat gtaagaggac ttgaagttgt
    45181 actaccaaat ggagaaatta taaatgtagg tggaaaggtg gttaaaaata gttctggata
    45241 tagtataaag gacttattag taggttcaga aggtacttta ggaatagtta ctaaagctat
    45301 attaaaattg cttcctttac caaagaaaag tataagtttg cttataccat ttccagattt
    45361 atcaatggca atagaaactg ttcctaaaat aataaaatca aaatcaatac caacggctat
    45421 agaatttatg gaaagagatg taatattggc agcagaagag tttttaggta aaaaatttcc
    45481 agataataca tcagatgctt atcttttact tacatttgat ggaaatagca ctgaagatat
    45541 agaaaaagaa tatgaaaaag tagctaattt atgtttagaa aatggtgcat tagatgtatt
    45601 tatatcagat acacaagaaa gaaacgattc tatatggtca gctagaggtg catttttaga
    45661 agctataaag gcatctacga cacaaatgga tgaatgtgat gttgttgttc caagagataa
    45721 gatagcagag tttataagat acactcacga gcttcaagac aaattaaaaa taagaataaa
    45781 aagttttggt catgcaggag atggaaatct acacatatat attttaaaag atggtatgga
    45841 tgataataca tggaaaataa gattaaaaga aacttttgac tatatgtata aaaaatctag
    45901 agaacttagt ggtcaagttt ctggtgagca tggcatagga tatgctaaaa aagagtactt
    45961 acatgaatca aattcagatg cttatatgat gttaattaaa aatataaagt tagcttttga
    46021 ccctaaaaat attttaaatc cagggaaaat atattagaaa tattaattta ataatattta
    46081 caaaacaaaa aatattaaat taggtttaaa tgtaatattt taaactttac ttttaaatct
    46141 gaagctaaat tgtaaaattt ttaggatggc aatatttact taaatttaga aagtaacata
    46201 atatttaagt tgatgttgtc atcctaaaag
//
LOCUS       DAEPXF010000009        45562 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain HB27
            SAMN13376389-rid12479423.denovo.010, whole genome shotgun sequence.
ACCESSION   DAEPXF010000009 DAEPXF010000000
VERSION     DAEPXF010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13376389
            Sequence Read Archive: SRR10518432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 45562)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 45562)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001069624.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001069624.1
            Genome Coverage       :: 315x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/15/2021 01:54:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,165
            CDSs (total)                      :: 4,076
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 89
            rRNAs                             :: 10, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 10 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 19 of 69
            Pseudo Genes (incomplete)         :: 44 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 10 of 69
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..45562
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13376389-rid12479423.denovo.010"
                     /strain="HB27"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="China: Hebei"
                     /collection_date="2017"
     gene            158..967
                     /locus_tag="KRL67_000460"
     CDS             158..967
                     /locus_tag="KRL67_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HBH1521365.1"
                     /translation="MKDYYKIGEISKIYGIGRDSLMYYEEIGILRPVRDINGYRMYNI
                     SDIWKLNLIKEFRSLNFPMKKIKEYLDDRSIESTKNILNEELDLIDKKIAEFISHKEN
                     IIKRLSSIESVIQNTKIDEIEVVYIEKRKALELNADIKRDEDFDFLIQKLQKEYEDRF
                     NILGNNNIGSAFSTEAINKGIFNEFKSVFCFLESNEKVYNIVFDEGYYVTLNYTGSNS
                     NNKMYMEKVFKFIEENNYKIIADPIEIYKIDIHETGIVEEFVTEIQVPITK"
     gene            1120..1359
                     /locus_tag="KRL67_000461"
     CDS             1120..1359
                     /locus_tag="KRL67_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH1521366.1"
                     /translation="MKCSNCGSVNIEKGILTTGGIYAETIGLKYVGDGKLDKVINKTF
                     PVQSTIYSDLCLDCGEIVRTYIKDNTDRNWCKDEK"
     gene            complement(1546..1722)
                     /locus_tag="KRL67_000462"
     CDS             complement(1546..1722)
                     /locus_tag="KRL67_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH1521367.1"
                     /translation="MFRDEMDKCTHMLTAYISSLYDYCDFIDTQLDDFILEYGENVVE
                     SCLHQVMVLVSKYN"
     regulatory      complement(1776..1872)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            complement(2214..2888)
                     /locus_tag="KRL67_000463"
     CDS             complement(2214..2888)
                     /locus_tag="KRL67_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HBH1521368.1"
                     /translation="MNHHILIQFEYIARIITAGFCGALIGYERKNRLKEAGIRTHFIV
                     ALASALIIIVSKYGFNDIVGTPGIGLDPARVAAQVVSGIGFLGAGLIFIRNQSVSGLT
                     TAAGIWATAGTGLAIGSGLYLVGIVSSIIIVIVQITLHRDRKWMKLPTAEQVSLKISN
                     STDSIEYIEGKFKKSNIEIINMNIKRLDGDWLEVVIYTKLPKGYKKTNLLKLFSDNPL
                     IETLEI"
     gene            complement(3108..3662)
                     /locus_tag="KRL67_000464"
     CDS             complement(3108..3662)
                     /locus_tag="KRL67_000464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HBH1521369.1"
                     /translation="MSIALILIDIQNDYFKNGKCELFQSEETAENAKKILLFFRKNNL
                     PIIHIQHISTDKTASFFLPNTHGSEINKIVFPLDNEEIITKHTPDSFFETDLQSILEK
                     KNITNLVICGMMSHMCIDTSVRTAKKLRYDITLISDACTTKNLSWNNIEINANTVHQV
                     FMASLQNSFADVKNTDKFLSNYYK"
     gene            complement(3740..4105)
                     /locus_tag="KRL67_000465"
     CDS             complement(3740..4105)
                     /locus_tag="KRL67_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBH1521370.1"
                     /translation="MKIRAEYTCPLEIVHDIIKGKWKTILIFELRQSNKTFSELQHNI
                     YGINQKMLIQQLKELRDFGIIDKISSAGYPLHVEYFLTNRGKKMLKAVEIMQEIGIEY
                     MLEHGQQEILDNKGICYNK"
     gene            4570..5355
                     /locus_tag="KRL67_000466"
     CDS             4570..5355
                     /locus_tag="KRL67_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBH1521371.1"
                     /translation="MIVKIDNTLEKEFWQYVSHEESLNLFIIGYVENYGFSSQYQDIW
                     FQVEDGNITSIILKNKSTLIIYSFKNNFDIGEMKNHIKDLDVESISGKKCVIDRLISK
                     YKDFYEKLDNKFCVLKEIKEIDFSNMKEYKIENAQEKDIDEIGKLLNRSDYKVSKNYI
                     EERKEHLKEGNVRAYFIRNDDTMISTVSTGIETSFLAMVVSVSTDKRYRGKGLASYMV
                     YNLSKELLLEGKVPCLFYNNDIAGKIYHNIGYKEVNEWTILFK"
     gene            complement(5451..6275)
                     /gene="pdaA"
                     /locus_tag="KRL67_000467"
     CDS             complement(5451..6275)
                     /gene="pdaA"
                     /locus_tag="KRL67_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="delta-lactam-biosynthetic de-N-acetylase"
                     /protein_id="HBH1521372.1"
                     /translation="MKKDSLKKYIMIGAFALILFGIASINFKSLDKTKTQISSPTLDT
                     HEYDWYFNPREDGKQPSPIKEADFFKKYGAYYVGNPNEKVIYLSFDAGYESGNTPKLL
                     DTLKKHNAKAQFFVVESYIKSNPDLIKRMEKEGHLVCNHSKSHPSMAGITDFEKFKEE
                     ITSVEKAYKDVTGKEMPKYFRPPMGKFSEQSLKYTQDLGYKSIFWSFAYVDWYEKKQP
                     THEFAKNKIYSRTHPGAIVLLHPNSSTNTEILDEVLTHWEKEGYKLKTLDYLNNKK"
     gene            6496..9615
                     /locus_tag="KRL67_000468"
     CDS             6496..9615
                     /locus_tag="KRL67_000468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease"
                     /protein_id="HBH1521373.1"
                     /translation="MDNIISVLNDVVFLDIEVTGLDCLNSEILEVGAVKVKDWKVYTY
                     ESLIKNKFEVPIEVFSVCRNLDKNDLEIANEIEMVEDRLVNFVEDNFIICHDLSLKKK
                     FFEYHMPKLKNKFIDLIELAVILEPYHKDYSLEYLKNTLTNCNSKVENRALSDAIDII
                     NIVNCLLVKFNNYEKTTLEPLSFKINSYLKKFNLPTWEWSRFLEEANYDLSNNINIKK
                     EYNIFDSKEEKKKERETLKILNEEEKNYEELLKYKTIWENKEGFTYEYRPGQYELTKT
                     IRELFRNSEDEEKIACIEAPTGIGKSVGYLLPAILEARINKKRLLISTDTKELQIQLI
                     NKDIPNVLDSLGLNGKVSYGYIKGKNNYICIDRLEAYIDDYESQNPTKGELLSLIFLK
                     RLVEGGKYGDIEEINYSVFDNFKEISTHLRNVSCDPNMCRPKKCKKDCLYKNRIEELK
                     EEHITVVNHSLLAKWPYKDEKPLENIIVDEAHNLTEKGYDFFSSIINSKSLRYLLQEI
                     YPYEFIQNSSFIYKKYSRNMRKIKAFDKFYNVLKIEREDKQKIARSINLIIEEIDSIL
                     NFGNCNEYNNVSNYNLRWELNLQIDEIVGKLKKDGVDTEISYRAYSEKIKLSCEKIIK
                     NLVSIIIIIYRNIDDDSIDKEADIYKFGKAKTRDLEDIKIIFEIFLEYDEKDDYARIV
                     EIDKNYNDFEFRVVPLKIADLFEENILSQLEKGIFLSATLSLSESMSYFKNTLGIDRV
                     KNVEKIIEPIFDYKNRVSVVGFSDICEYRNSEFPNEMSKIISNISKITEGHTLALFNS
                     KDRQEKTYEILKKYLHSFNLEIYADKKGIRHLNDLNRKCVVLGSKGCFEGVDIPGDGL
                     VCVTLDKLPNLNPKDPLYFTIMKKYSIDYYTINYPQMTIKVKQAMGRILRSKYDYGCF
                     VIFDVGTNLSVLKRLEKDLHDCKISKVNSNEFYTYIRRHLNKSRSLILKSVIFDTIKA
                     LNVDAKMDNNDVDKDIIKKDINENIRQRAVKGEVYHIDIIKKDMKVKYFDRNYLINLD
                     IFMREEDKN"
     gene            9633..10232
                     /locus_tag="KRL67_000469"
     CDS             9633..10232
                     /locus_tag="KRL67_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease H family protein"
                     /protein_id="HBH1521374.1"
                     /translation="MAKQKFYAVKKGKNIGVYNTWDECKKQVNGFSGAEYKSFSTFQE
                     AKEYIYGSEKLSFQEDKEFIEAYVDGSYEHSIKMYGSGVVILKNNEVIKTYSEKGKEE
                     SLVSMRNVAGEIEASKIAMQYCIDNNVQNLILYFDYEGIEKWCTGVWKTNKKGTIAYK
                     NFYDSIKNKLNVKFTKVKAHSGNKYNEEADKLAKKAIGV"
     gene            complement(10474..12603)
                     /locus_tag="KRL67_000470"
     CDS             complement(10474..12603)
                     /locus_tag="KRL67_000470"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HBH1521375.1"
                     /translation="MPNLPSLGSKAPDFKANTTNGPIRLSDYKGNWIVLFSHPGDFTP
                     VCTTEFLCFAKYYDEFKKRNTELIGLSVDSNSSHLAWMYNISLLTGVEIPFPIIEDRD
                     MRIAKLYGMISKPMSDTSTVRSVFIIDNNQILRTILYYPLTTGRNIPEILRIVDALQT
                     SDRDNIVTPANWFPGMPVILPYPKNYKELKNRVNSCNKKYSCMDWYLCFVPDNYNDEE
                     VNKKIDNTCSWKKEHTKNIENECNCEHEHHDYLNKALDCKQENKTDIKNDCNHEKKHT
                     KNTNKVHNSKQDKFKDKSCDEMNFNYDKDESCDKINSSYDKEDSSYEDFYKHNYKNYD
                     YTGEKNTKKIAMKTLKDSKKLIRPQITDPYNPIVENANCPDINPIVAEYVLGNPTNVD
                     AQLLDAVIFAFAEIDQSGNLFIPYPRFLNQLLALKGEKPSLKVIVAIGGWGAEGFSDA
                     ALTPTSRYNFARQVNQMINEYALDGIDIDWEYPGSSASGITSRPQDRENFTLLLTAIR
                     DVIGDDKWLSVAGTGDRGYINSSAEIDKIAPIIDYFNLMSYDFTAGETGPNGRKHQAN
                     LFDSDLSLPGYSVDAMVRNLENAGMPSEKILLGIPFYGRLGATITRTYDELRRDYINK
                     NGYEYRFDNTAQVPYLVKDGDFAMSYDDALSIFLKTQYVLRNCLGGVFSWTSTYDQAN
                     ILARTMSIGINDPEVLKEELEGIYGQF"
     gene            12869..13117
                     /locus_tag="KRL67_000471"
     CDS             12869..13117
                     /locus_tag="KRL67_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH1521376.1"
                     /translation="MMICFAMTFALLLINSVMGVHIGLDYYFLMGSVLTVCLTIGIVL
                     CNARKIFDSKPIHKTRTKTVSTNKKQKSHSRDIKRKIS"
     gene            13283..14674
                     /locus_tag="KRL67_000472"
     CDS             13283..14674
                     /locus_tag="KRL67_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HBH1521377.1"
                     /translation="MNFRDLFEKAVDFIDEKRKSIVAISLSILGVIVLIIVFFLSSNE
                     FSVGNEANELLKIIEKRQYSIAVDYYTSIEKKFSDSKMERFNKSVSKKINKLLLNSGD
                     KYLDGDISQESFIGLINTVKELKKISIDVDDLLTQADRVREMYKEENTTYDIAINYIN
                     TVSILNGIGSNLDVYKQNIETIKESRDVYDSAVKDQKAYKYYEAIEKYNKVLKEDEKY
                     YSMAQNGKEQCIEEMYDYYISRAEEANTSGDYERALQYIEYLKEDYSDDEKVQSLEKK
                     YKKNLSLYTLTSDDIINVIAKKSGKDKANLSINSLPQMIKNNKYYYAEVYEYNKLINE
                     VLVSAKTKDLYSYKDGKKDYKVDYGDGYFRILEDGSYQFGITKDKAKFVLTNTLDEKE
                     NKYKKIEILDIEKADRYVKSKKSLEELFGKYKNIYYYAVVNKGLFRGKEVYAINIYNE
                     KIYAISENGLNEY"
     gene            14789..16258
                     /locus_tag="KRL67_000473"
     CDS             14789..16258
                     /locus_tag="KRL67_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanW family protein"
                     /protein_id="HBH1521378.1"
                     /translation="MSNVNKKLVIAIGAVALVVIIFIGIMCIQFKGEKIAKNTYVNGV
                     NIGKLTKSQAKQELAKKYKLENVEFNYNDKSWKVKSKDLNLSTDLDKTVENAYNLNRK
                     SGFFGNLSKTISANFGKKSNLVVVINYDKNKLKAEMEKIAKEIDVDVKDATLDISGEK
                     VKVIPDSDGLKMDISKSMENFDNQTKKGNYKNELVVKATPAKVKKEQLANIDTNLGTY
                     STTFKTSQINRSINIKLATDNISNVLLMPGETFSFNKHTGKRSKENGYKSAPVIMEGE
                     MEEDYGGGVCQVSSTLYNSVLYAGLEIVNVKNHTIPSSYVPKGRDATVADSGIDFLFK
                     NNLKHPVYIKNYVSGNQIVCNIYGSAEDKQNITISTKLDGVSQTTMKRVNDPTMPKGK
                     EKVDKSGRNAYSVSTYRTFNDANGKKIKTEKIANSYYPKKEGIILVGTMEPKPEEKPN
                     IDENKNNQNTNNQNTNNQQKPETPPTDNKPNETQPQPQA"
     gene            16323..16916
                     /locus_tag="KRL67_000474"
     CDS             16323..16916
                     /locus_tag="KRL67_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fis family transcriptional regulator"
                     /protein_id="HBH1521379.1"
                     /translation="MNLNNITNYVQSICENISNVLDVDVTLVTKDLTRIAGTGIFRGK
                     IGETISEKSVYSLVLKDGKSYVINKEIEENCNKCANRENCKELADICTPVKMGEHILG
                     ILGIAAFNEEQKEKILSKDKELTAFVRSMSDLISFKLDELYKQEVKTIDDLEREAIEN
                     AIKKYGSNTEGMKKVAEALNIGIATLYRKVKKFNIKS"
     gene            17098..18444
                     /locus_tag="KRL67_000475"
     CDS             17098..18444
                     /locus_tag="KRL67_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HBH1521380.1"
                     /translation="MPRKILDISLIEDNKSDLTFFNIEEEDGDETLKALKQSYVTTNY
                     KEETLLNLQGALNSKSDTTSKELKLESYKRYNNALDLAYKNYITSAINMVNKALEINP
                     KDIDILNLRGLLKLLKCDFAKSFESFYTALCYENNHISRKYVNLISSEDFKTFLGRYN
                     HSIRFINEELNYESIHILENIIEEEPELIEPYVILSLLYDKLGNVKKREGYLDKLKEL
                     DKDNPVFEKNEEEKEDTSKKEEIKKVKRKKSILPYIIVGCVLIGMGIYLIQSKKRIEN
                     LNNQISSKEEKLSETDKKLGETSKKLEKTNKELNEVKNKEPKREITVADEEDLYYQAL
                     DLKKNKEYEKAIDNFKSVVSSGKTKKYISESIYQLAITNKLLGNKDEAIKYYKKYINT
                     YTKNDQYYDDSYYELGMLYYDNGDLKNAQQTFYSLRSEVPDSMYNNSKIKEILSEK"
     gene            18626..18997
                     /locus_tag="KRL67_000476"
     CDS             18626..18997
                     /locus_tag="KRL67_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HBH1521381.1"
                     /translation="MKVFNQLVIILSIWVIGEYISSFIQGIILIPGSIVGMLLLFLLL
                     QFKVLDLSSIENVSGFFLDNMAIFFIPAGVSLIKSLDLIRENVLVLLLVICLSTLIVM
                     YTTGIIVEKMIKKKEEGQKNV"
     gene            18990..19679
                     /locus_tag="KRL67_000477"
     CDS             18990..19679
                     /locus_tag="KRL67_000477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LrgB family protein"
                     /protein_id="HBH1521382.1"
                     /translation="MYNVLQTPVFGIIVTIVFFNLGRYIQRKTSNPICNPLLIAIVGI
                     ILFLSISKIPYENYKIGADSLNFFLGPVTVVLAVPLYKQFELFKKHMFEIIVGIGCGI
                     VISFVSVLIIGKIANSDVSIINSLIPKSITTPMGISLANSLNGIESITVVAIIFTGIF
                     GGMVASTVFKLGNINHPVAKGIALGTSAHALGTTKAFELGEVEGAMSGVSIGVSGTIT
                     VILIPIIMNFI"
     gene            20200..21066
                     /locus_tag="KRL67_000478"
     CDS             20200..21066
                     /locus_tag="KRL67_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HBH1521383.1"
                     /translation="MSEVKNTLQKHPVLRYSTMLFGMLMTSIGINGFLRPAHLLSGGA
                     TGIATSINYLTNINVGLLTFLINIPIFILGFIYLEKEFCISSLVNMIVFSLLLGATQE
                     ISNIIPIHDILLQSVYGGILSGLGVGVVFRTRSSQGGTDIIAAILKIKKNIEMKDTAL
                     AINGLIVLTGSFLFGLDLALYTLIGLFLNAYSMSFIKDAMNYQKSVMVMSNEVDLIAE
                     DIMKSLVRGVTFLDAEGAYTHQKKKIIYTIVSSNEIPKIKDIALKYDKKAFISVNDVT
                     EVKGRGFKAKDL"
     gene            21308..22117
                     /locus_tag="KRL67_000479"
     CDS             21308..22117
                     /locus_tag="KRL67_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH1521384.1"
                     /translation="MNLSKEYVNLYDNYLKYINEVNKEIRSIKNMKKRVLKRGDFAPE
                     NEVLELEGKTIRDIDDVDTKKPKNKIYKFSNGDVYIGKFLEGKMEGKGSYTFFVDEGT
                     VMEYIGEFKEDKKNGKGNFVFSNGNEYVGYFEEDVMSGIGNMLYNSKDEYIGTWKNGK
                     KDGKGIYKWSDGCIYAGEFKGGKMEGYGICYNSKGEVLYEGEWKNNLIHGKGTYIWEK
                     GKKYIGEFMHGKKHGQGTFYLNNELVYEGTWKFDKPSIFDRTLDEIFSLRL"
     gene            22261..23325
                     /locus_tag="KRL67_000480"
     CDS             22261..23325
                     /locus_tag="KRL67_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HBH1521385.1"
                     /translation="MKRLIALVLVVLIVLFYSPELLKEGYDLKEKFFQSEIWNEFLDK
                     ININDNSKKDKKEKSQNLQSNNTEGENLGESDIKNFEKISLGDNLSYVLSSIGKPGRI
                     DISEYGFDWYVYNQYGKEFAMVGLENDEVVALYSNSINSCENQDIKINQDRQTVRTKI
                     TPLKYKRKGNTRYIINSENQYDIISKEGKYITIFYDIHEENRVCSYLIIDKSTEDEFK
                     NLYPDDSEELKKCFELEIIDLVNSVRNQRGLNSLRYSEQATLSSRKHSEDMRDNNFFD
                     HVNKKNETPFDRMKREGIVYTSAGENIAAGQINAIYAHEAWMNSEGHRKNILGNYNNI
                     GVGVIFGGSYKTYYTQNFYK"
     gene            23353..24072
                     /locus_tag="KRL67_000481"
     CDS             23353..24072
                     /locus_tag="KRL67_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HBH1521386.1"
                     /translation="MTSWKKKTVYKCLIAVVLFCGIVLISNFSKVSALMMDTNGNVLI
                     KHGSREKKLIAITFDDGPHPKETSQVLDVLKKYNVKATFFIAGKHAKWYKEPLVRASK
                     EGHEIGNHTFNHPDISNLSSSQIEEEIVKCEDILKEVTGKKPTLFRPPFGSYREKDLI
                     EIAKKHDYKVVLWTGVDVKDWKNPGANSIADKIINKVQNGDIILLHDYATNDTVEALD
                     MFIPKMIEKGFKFVTVSELIK"
     gene            24095..24679
                     /locus_tag="KRL67_000482"
     CDS             24095..24679
                     /locus_tag="KRL67_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HBH1521387.1"
                     /translation="MILMIDNYDSFVYNLVQYIEELGETVVVKRNNEIKISDIEELNP
                     EVIVLSPGPCSPKEAGICIDVVEHFKGKKPILGICLGHQTIGHVFGGDIIKAQQPVHG
                     KVYSISHTNKGVFSGLKNPLNVTRYHSLIIDSNTVPKELEITATTDKGEIMGIRHKKY
                     LIEGVQFHPEAILSEYGHEMLKNFITEARERVHM"
     gene            24681..26030
                     /gene="pabB"
                     /locus_tag="KRL67_000483"
     CDS             24681..26030
                     /gene="pabB"
                     /locus_tag="KRL67_000483"
                     /EC_number="2.6.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component I"
                     /protein_id="HBH1521388.1"
                     /translation="MIREINTKLNSFEIFTIFRNEHDSFILDSAMDKEKLGRYSFISS
                     QPFKVLKYKDTDENPLEVLKEELHKYRVANDTNLPFVGGAVGYLSYDLGNYIENLPRT
                     AVDDIEMPDMYFGFYNHVIVIDHLVQKTYIATPNIDIELEEKIMDDIEQRILKEEKKG
                     IDSICYEEKEVTPIRLKSNFTKEEFKNAVQSVREYIRQGDIYQANLTQRFSGETELTS
                     FELYRDLRRFSPAPFGAFLNFEDTHILSNSPERFIKCVNKRIETRPIKGTRPRGKDKE
                     EDLKLQQELRNSEKDRAELLMIVDLERNDIGRISKTGSVKVPELFVIEPYANVNHLVS
                     TVVGELKDDKDATDVIKATFPGGSITGAPKIRAMEIIDELEPTQRNVYTGSIGYIGFN
                     GDMDFNIAIRTIIKNDKKVYFQVGGGMTWDSDPDEEYQETLDKAKSIMKALRGYYEE"
     gene            26020..26763
                     /locus_tag="KRL67_000484"
     CDS             26020..26763
                     /locus_tag="KRL67_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class IV family protein"
                     /protein_id="HBH1521389.1"
                     /translation="MKNNVGFDSSLSKFGIGLFETIRVKNGIAIDLNIHIERMISSIN
                     CLDLDINYKKDFLINEIVTYIKKENVINKALRITVFDEGYNISIRDIPYNQETYEKGF
                     KLAISPIVRGNSLIHRHKTTNYFENIYTKNIANKTGYNDGIFLNSEGVILECSMSNIF
                     FIKGERVCTPSDRLPILNGIIKKRIIEICDELHIELIENEINISEISSFDFVFVTNSL
                     MGAIKVTEIDKIKFNKENVVFDKIVKLLE"
     gene            26797..27498
                     /locus_tag="KRL67_000485"
     CDS             26797..27498
                     /locus_tag="KRL67_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH1521390.1"
                     /translation="MIGCIYEVYNEEMTKIIENQNTLAVYLVGSSKDVDFTLYDVEIN
                     DIDVFVFVKEGEKQERILFKKKGIEFDINYFSKDGVKKLLSNREYFFVKEMKDAKVLF
                     DRESISHIIKDISRNIYLEGPSKISLEEKSFIKQDIGAKISNLKNKEKFDVFEYHFLT
                     NLYLKDIIIGYFNINDKWVPKDKKLLKVLKKENNELFELVSKVSENYDYQRLLDVYNY
                     IFKEIETKEVIKLIF"
     gene            27524..28096
                     /gene="folE"
                     /locus_tag="KRL67_000486"
     CDS             27524..28096
                     /gene="folE"
                     /locus_tag="KRL67_000486"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HBH1521391.1"
                     /translation="MNKVDKEKIQHAVREILEAIGEDPDREGLIETPNRVARMYEEIF
                     SGLSEEPRDHLKVLFADEKHEELVLVKDIPFYSCCEHHLVPFFGKAHIAYLPKGGRLT
                     GLSKLARVIDTLAKRPQLQERITKNAADIIMEELQPYGVLVVVEAEHMCMTMRGVKKP
                     GSKTVTSAVRGIFEKDIASRAEAMSLITMK"
     gene            28162..28953
                     /gene="folP"
                     /locus_tag="KRL67_000487"
     CDS             28162..28953
                     /gene="folP"
                     /locus_tag="KRL67_000487"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HBH1521392.1"
                     /translation="MFDYGKRTYIMGILNVTPDSFSDGGDFNNLDIAIQHAKDMVDQG
                     ADIIDLGGESTRPGHSYVDSEEELRRVIPVIKKLKQELDIPISIDTYKADVAEEALKL
                     GVTMVNDVWGLRRDKNMASVIGKYDAEVCIMHNQDGTNYDKDIIASIKEFLKESIEMA
                     ISCGVKKEKIVLDPGIGFGKDFEQNIEVLRRLNELKDLGYPILLGTSRKSVIGKVLPV
                     EPKKRLEGTIATTVLGIRDGVDIVRVHDVYENLMAARMTDAIYRK"
     gene            28964..29329
                     /gene="folB"
                     /locus_tag="KRL67_000488"
     CDS             28964..29329
                     /gene="folB"
                     /locus_tag="KRL67_000488"
                     /EC_number="4.1.2.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HBH1521393.1"
                     /translation="MDKILLSNLGFYGYHGVLKEENFLGQKFFVDMELYIDSREAGLS
                     DDINKSVSYAEVYNVVKDITENKQFNLLEALAENIAEEVLNKFILINGVMVRVRKPEA
                     PVNGIYDYFGVEIRRTRDE"
     gene            29322..29828
                     /gene="folK"
                     /locus_tag="KRL67_000489"
     CDS             29322..29828
                     /gene="folK"
                     /locus_tag="KRL67_000489"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HBH1521394.1"
                     /translation="MNKVYLGIGTNMGDRFDNLSRACELLKNSDSIYKVKESSLYETK
                     PWGYTEQADFLNMCVEIETEFEPYELLEYCQEIERELHRERIVHWGPRTIDVDILFFN
                     DVVSTDEKLTIPHPRIQDRAFVLIPLMDLNEELIINEKTIKEHLNLLSAEEREEVKEL
                     VGYERKPI"
     gene            29809..30594
                     /gene="aroF"
                     /locus_tag="KRL67_000490"
     CDS             29809..30594
                     /gene="aroF"
                     /locus_tag="KRL67_000490"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HBH1521395.1"
                     /translation="MKENLFNNNSKIEINTNNKKITVKKDKLIIAGPCAIESYEQLLE
                     TAKFVKSQGANILRGGAYKPRTSPNSFQGLKKEGLEILKAVKDEVGMAVITELMDVRD
                     MDELYSISDIIQIGSRNMQNFTLLSEVGKQDKPIMLKRGIASTITEWIGASEYIAIEG
                     NSNIIMCERGIRTYNDYTRNTLDLAAVPIIQKETGLPVVVDPSHATGVRYLVKPMSLA
                     SFACGADGIMVEVHPDPENALSDGAQSLCFNEFEDLMKSINNY"
     gene            30849..32639
                     /locus_tag="KRL67_000491"
     CDS             30849..32639
                     /locus_tag="KRL67_000491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HBH1521396.1"
                     /translation="MNDIKDIIEEIKARCDIASIISEYMNIKQSGANYKGLCPFHGEK
                     TPSFYINTSKQIYKCFGCGEGGDVINFVMKMENLDFMDAVKILANKCGIEINTNMNEE
                     TRIKIEKSKKFQDIHTEAARFYLSNLLGSKNLGYEYLRIRGLDDKIIKKFGLGFSLDS
                     WNSLMNTLISKGYKKQDLLECGLIAKNRDGTNCYDKFRNRVMFPIFDYRGNIIGFGGR
                     VLDDSLPKYLNSPDTLIFNKKQNLYGLNFARKNLESKTIVLVEGYMDLISLYQYGIKN
                     VVATLGTALTEQQGILIKRYADTAIISYDSDEAGIKATLRAIDILTKLGINVKVLDLK
                     DAKDPDEFVRKYGLSDYKKAMDVSTHYIKYKIDHLKKEFNIQKDEERVKFAKEASKII
                     KQLTSPVEIDFYTKYLSNQIDINVESIKREVYGKNYNKPYNNKNQKKIEEKVIEKVEV
                     RQDGKQLVEETLIKIMLEDKKIREIALLKVEESDFLLKESKEILNYMIKNQELDKITI
                     DKLKSLNISEEYLKELNSISLNSINLENTKEIEGIITNIRKNSLEEQINSLLREQQEL
                     ENNNDMKEVDGRVMEIALKIVEINKILKSL"
     gene            32657..33823
                     /gene="rpoD"
                     /locus_tag="KRL67_000492"
     CDS             32657..33823
                     /gene="rpoD"
                     /locus_tag="KRL67_000492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HBH1521397.1"
                     /translation="MENKSNKKELKKVTAKTLIEKGKKQGSLTLAEIMEAFSETELDK
                     DQVENLYETLGNLGIEITETKNYKADIDFSVADDDLSIGHLDEDAEAISHDDSSAIEI
                     ETVDLSLPKGISIDDPVRMYLKEIGKIPLLKPHEEVEFARRMHEGDEIAKQRLVEANL
                     RLVVSIAKRYVGRGMLFLDLIQEGNLGLIKAVEKFDYTKGYKFSTYATWWIRQAITRA
                     IADQARTIRIPVHMVETINKLIRVSRQLLQELGRDPKPEEIAKEMEMTEDKVREIMKI
                     AQDPVSLETPIGEEEDSHLGDFIPDDDAPAPAEAAAYSLLKEQIEDVLGSLNDREQKV
                     LKLRFGLEDGRARTLEEVGKEFDVTRERIRQIEAKALRKLRHPSRSKKLRDYLD"
     gene            33988..34677
                     /locus_tag="KRL67_000493"
     CDS             33988..34677
                     /locus_tag="KRL67_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HBH1521398.1"
                     /translation="MKLTDRLLKIASLVSDGKKIADIGTDHGYIPVYLLKEGKVPFAV
                     LADVNKGPLDNAHKEVIQNNLLDKVDLRLGSGIEILEIGEVEEVIIAGMGGILISELL
                     EAKKEVAHNVEKLILQPMQAQEELRYYLLNNGYEILEEVLVREDFRIYEIIVAKYTGK
                     NTIIEDEIYYEVGIKLLENKDSLFNDFIEKKIKTYSSIVNKLEGKNGEAIDKKRKESE
                     VVIKKLENLIK"
     gene            34691..35788
                     /locus_tag="KRL67_000494"
     CDS             34691..35788
                     /locus_tag="KRL67_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HBH1521399.1"
                     /translation="MLLKSLTRKIEKKYPLNLAEDWDNVGLIVGDFDMDVKKVLVSLE
                     ANEDVIDEAISKNIDLIVTHHPFIFGKINKINSGDLKGRLIQKLIKNDISLYSMHTNF
                     DIAFDGLNDYFMEIMEFGNSKVLDITKSENLYKLAVYVPHNYSDELRKVLSNSGAGHI
                     GNYSDCTFSIEGEGQFKPLEGSNPFLGSVNDIETVNEVKIETVVPQKLLGGVISSMLD
                     AHPYEEVAYDLYKLENKGEIFGLGRISKLDKSMTLESLSKKIKEKLNMKHIRVVGNLS
                     TNITKVAVVTGAGSEFVKKAKRQGAEVLITGDVKYHEAQDALDIGMCIVDCGHFDTED
                     IFKNVMKRFLDEFSEIEVIKSNVYLNPFSTI"
     gene            35851..36423
                     /locus_tag="KRL67_000495"
     CDS             35851..36423
                     /locus_tag="KRL67_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HBH1521400.1"
                     /translation="MKVAVIFHSVCGSTYLLAREYKEVLEEMNIEVDIFRVSDEVAKT
                     LPQYYLINSKEYKDEFESIDVIKSGKELLDYDAIFMGSPTYYGNVSGPMKMFMDSFSD
                     IWVGAPLSGKIFGCFATAGSQHGGGELALQAMNIFAQHMGMTLLSVPCSVRGGHPAYG
                     ILHIAGDNSDIRPSDDVKLGIRDYLKRLNI"
     gene            complement(36483..36950)
                     /locus_tag="KRL67_000496"
     CDS             complement(36483..36950)
                     /locus_tag="KRL67_000496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HBH1521401.1"
                     /translation="MFKEMRLKKREMTKEDTVEVLKNGEFGTFSTISENGYPYGVAVN
                     YVYFNDSIYFHCARNGHKLDNISKNNKVSFLVVANESVIPDKFSTTYSSAIVFGKACT
                     VENEEKKNALVEIIKKYSKGFFEEGMKYIEKDMNLTTVVKIEIDHISGKASRL"
     gene            complement(37336..37812)
                     /locus_tag="KRL67_000497"
     CDS             complement(37336..37812)
                     /locus_tag="KRL67_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HBH1521402.1"
                     /translation="MLSKAEIKYFNECASEILSSEKVQLMRTFPHHGNVSCLEHSLSV
                     AYYSYLLCKKLHLSVDIQSVIRGALLHDFFLYDWHYKGDRKGLHGFTHPREALKNATL
                     FFQINEKETDIILKHMWPLTVKPPRYKEAFIVCLLDKFCCLVETLKIHSLLSPYHV"
     gene            complement(37932..39023)
                     /locus_tag="KRL67_000498"
     CDS             complement(37932..39023)
                     /locus_tag="KRL67_000498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1648 domain-containing protein"
                     /protein_id="HBH1521403.1"
                     /translation="MSKIECLFTIFPVLILMYATFYFMPYFVGKKHIYGVSIDQEHKN
                     YNYFIKLDKEFKKLLSLGFIIDFILVFILVFIFNKLELSYFIGIIGFLLYESILYIHT
                     HKKAKKLKSEIYSKLGHIDIDSKLIVDMDFINKKNKIIKKFKIIYLIPILFTFGMSIF
                     IAFNYNKLPDSIATHWNINGSPDVFIDKSFLNVFKLIGTDFCLMVLLYITSIGSIKSR
                     IKIDTNRIEESRIENIKYLNKIGYLFLILMIIMTTQFFTTLLSIKTKLSTAMNITTLL
                     VIIYLIATYINSPNLKFNSSYSPEEDEKYWIAGIMYNNPNDPSLMVNKRFGIGWTINF
                     GNPLGKILYIAIALLLIFSLFSLIKSLLL"
     gene            complement(39016..39405)
                     /locus_tag="KRL67_000499"
     CDS             complement(39016..39405)
                     /locus_tag="KRL67_000499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBH1521404.1"
                     /translation="MVLNLDFNSDKSIYVQIKEEITKAIASGELKINESLPSVRSMAE
                     NIGVNLHTVNKSYNELKEEGFLNIDRRKGAIVAQLPMKIDNTTRQKNKLDLELLVAKS
                     YLSGISKEEFLSLSSSLFDKYEVKYYE"
     gene            39597..40043
                     /locus_tag="KRL67_000500"
     CDS             39597..40043
                     /locus_tag="KRL67_000500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH1521405.1"
                     /translation="MLTYLMKRQMHKKDDGMSKPMMITGAVITGVGAYAVYKMVKSRK
                     SNSQMVNTNYYGNSDYDDYKYDEFDYDCDCRDTKQDYEYEELKKKVKEFNSRRINCEN
                     CPHVSQEEMMQYVNSVKDVKDEIDLHEDAKKDNIYKEDEYKKYEEE"
     gene            40328..41032
                     /locus_tag="KRL67_000501"
     CDS             40328..41032
                     /locus_tag="KRL67_000501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBH1521406.1"
                     /translation="MLKLLIVEDDSTISFGIKYALEQEGFSIDISKDLSSGKEMISSN
                     EYSMILLDVTLPDGTGYELCQYIRGFSQVPIIFLTACDEEVNIVMGLDIGGDDYITKP
                     FRIRELISRIKAVLRRKVNTVEESKKILKFGELSIYTLEARVYKNDKEIFLTSVEYKL
                     LLILIQNKNTVLSRTQILEKLWDVTYDFVNDNTLTVYIKRLREKIGDDLCEPIYIETV
                     RGIGYKWIGSENSVSI"
     gene            41019..42263
                     /locus_tag="KRL67_000502"
     CDS             41019..42263
                     /locus_tag="KRL67_000502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBH1521407.1"
                     /translation="MYLYNPEVKKFLSKYATLMFIVIIISIGFSVINVSLTKEMVVRN
                     NQAIIGTLSSKYPNLESDIVDIITQGKSTENIDYGQKILSKYNYDKSIKINSEPIISK
                     LVLETIKINIILVCIIFILILVLVVRYFKSIYNDLSDMTKYVYYSSEGKPFDMKNKNQ
                     EGQIGLLKTELLKMTTILNEKVELLKREKIFLNNTISDISHQLKTPMTSLIMLNDLLY
                     NDVPYEVKIDFLNKIKNQLNRMDWLIKSMLKLSKVEAKVINFKKDKVKFSELIHRAMQ
                     SMKIPMEIKNQNLTIEGNDNVSYIGDIDWSVEALVNIIKNCVEHTPEFGNIIITYKEN
                     PLFSELIIKDEGEGIDKKDIPHVFKRFYRGRSSSKEDSVGIGLAMSKSIIESQSGDIY
                     VNSEKGKGTEFHIIFHKMYDSD"
     gene            42343..43020
                     /locus_tag="KRL67_000503"
     CDS             42343..43020
                     /locus_tag="KRL67_000503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003517588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBH1521408.1"
                     /translation="MEILRVENLTKSYGKNETKVDAIKNVSLSVEKGTFIAITGPSGS
                     GKSTLLHLLGGVDRPTSGKVYINDVDIYNLKTKDLAIFRRRNIGLIYQFYNLIPVLNV
                     KENILLPAELDNRDIDGEYFDDLMETLGLIDREKHLPNQLSGGQQQRTSIGRALINRP
                     SIILADEPTGNLDSKNSKEILELLKLSVKKYNQTLIMITHDKNMALQADRIISIEDGI
                     IKSDEVM"
     gene            43021..>45562
                     /locus_tag="KRL67_000504"
     CDS             43021..>45562
                     /locus_tag="KRL67_000504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBH1521409.1"
                     /translation="MNLYTSLTIRYLKQNKRRTIVTIIGIILSTALICGIGNIFESFM
                     DYQIRETIKNDGSFHVTFYDVNRKNVEYVTKSAEVEKHAFTKQLGYSKLENSENGIVS
                     IKQYDKNAINGYKISIKEGRFPAKEGEIVLSDNIINLIDDNLKIGDKITLKVGDMFDS
                     SKKKIDSMVFHEGDYIANEKDRTFEVVGIIKKPGFERYNGIATAITYFNPMDNYNDTI
                     NVSVALRNPKDVYEISNKISKNIYSNKDKEAVSDMIKYNEHLLRLQGASKYANINSSI
                     KSIIIAVTILVIICTIATVYNSFSISITERKKQFGILNSIGATSSQIKRLVFIEGIII
                     SLIGIPIGIISGTVAIDLLFKIINKHFTESVVTQMSLKIVYNPIIIIVSVIIVLFTIF
                     ISILLPAISASNISPLNVIKNNGEYKVGKVKSSRLIKMIFKTEGVLAYKNIRRNRKKF
                     TVTLFSLMVSIIIFISFSGFTLLLLKGEEIRNSQRNYDLYLTAKGTAKSIDDTISKLE
                     AISGIKNFELATGQYMSIRVSENKINKSKEDLIRKYYQKYKIGDSYEYDFSNNELIFP
                     GDFAVKNKTNNLIQGSFNKERAIEENGVILVRKSAFEENGKKGVVELTNYKVGDTVNC
                     EYLDENALSKKIKVKILAITDEERLGLGYQNMGLQFITYDEVAKNLNLKLSKSLIFID
                     SGGSIKTKKEIEALANKNNFNFYDESASNESEKQDLKVIKIFVYGFIVVISLVSVTNI
                     LNTVSTSINLRKRELAVIQSIGVTPKGFRKMIYLESFIYGILSLLFGIPISIGIILIM
                     NKLISGVIEFSPIIPWTAIVICIVSVFIITFVAGYIPMSKLNKENIIDN"
BASE COUNT        18486 a         4634 c         7657 g        14785 t
ORIGIN      
        1 attacaatat aaaaatattt taataattga ttataggatg tttattttcc tatccttgtt
       61 ttagagattt ttttagtata ttctaatact atagtattat agagtaataa tgttgattta
      121 aaataaaaat aggatttcgg aggagtttat tatataaatg aaagattact ataaaatagg
      181 agagatatct aaaatatatg ggataggtag agattcatta atgtattatg aagaaatagg
      241 cattctaaga ccagttagag atataaatgg atatagaatg tataatatat cagatatatg
      301 gaagttaaat ttaataaaag aatttagaag cttgaacttt ccaatgaaga aaataaaaga
      361 atacctagat gacagaagta tagagtctac gaaaaatatt ttaaatgaag aacttgattt
      421 aattgataaa aaaatagctg aatttataag ccataaggaa aatattataa aaagattgag
      481 ttcgattgaa agtgtaattc aaaatacaaa aatagatgag atagaggttg tatatataga
      541 aaaaagaaaa gctttagaat taaatgctga tataaagagg gatgaagact ttgatttttt
      601 aattcaaaaa cttcagaaag aatatgaaga tagatttaat atacttggaa acaacaatat
      661 tggttctgcc ttttctactg aagcaattaa taaaggtata tttaatgagt ttaagtctgt
      721 attttgtttt ctggaaagta atgagaaagt atataatata gtttttgatg aaggatatta
      781 tgtaacttta aattacactg ggagtaattc taataataag atgtatatgg agaaggtgtt
      841 taaatttata gaagaaaaca attataagat aattgctgac cctatagaaa tctacaagat
      901 agatatacat gagactggaa ttgtagagga gtttgtaaca gaaattcaag ttcctataac
      961 taaatagcta tatttcaatg gattagatgg taaatagaat gaaaaaatgt tgatttgcca
     1021 ccgatttgcc actgtgttat aaaatcggta gcaaattata ttttaaatta taagatagca
     1081 tgataacatg aaaaaataat tgtttaggga gttataaatg tgaaatgttc taattgtgga
     1141 agtgtaaata ttgaaaaggg aattttaaca actggtggca tatatgctga aactattgga
     1201 ttaaaatatg taggagatgg aaaattagat aaagttataa ataaaacttt tccagtacaa
     1261 tctactatat attcagattt atgtttagac tgtggtgaaa tagttagaac ctatataaag
     1321 gataatactg ataggaattg gtgtaaagat gagaaataga atagtttttg aagtatatta
     1381 atatttgaag ttgaatttta ttaaagatac aagtgaaaaa gtataaatgg gaagaaattg
     1441 ttatatggaa gaaactttgc atataggaga gagtagtttt ttaatgtaat aacattttta
     1501 aatgtagata aaactaaaaa gggttctcta gaaaacaatt tctatttaat tatacttact
     1561 taccaatacc atcacttgat gtaaacaaga ttctactaca ttttctccat actctagtat
     1621 aaaatcatct agctgtgtat ctataaaatc acaataatca tataaactac tgatataagc
     1681 agttaacatg tgtgtacatt tatccatttc atctctaaac atatatatta ctatccccct
     1741 aaaaaacttt cataaaacaa aaagaacaat atctttttgg caactggctg acataactca
     1801 tagtttattt tataagcgtt agtccctata gctttgcgtc actaaatttc tctagttttg
     1861 ccgatttagt tttattctac aactaaaata atacactagg ataagttatt gttcaacata
     1921 cttgtcagaa aattgaaata tttaacaata tcttaacaat atgaaatcta ttttacattt
     1981 aagaaacaaa ctatacaagt agaatactta ataaaagtta tatttagttt ttgtattaaa
     2041 aagtttagtt cacaaaggtt actttgctag ataatagatg aacaaaaata tcttgaaaaa
     2101 atatatatga atggcaaaaa acaagggatg aattttatat agactcatcc cttaattatt
     2161 tatatattga ctttgattaa atatattatt atttttaagc ttcaattttt atattatatt
     2221 tctaaagttt ctataagagg attatcgcta aataatttta ataaatttgt ttttttatat
     2281 cctttaggta acttagtata tataactact tctaaccaat ccccgtcaag acgtttaata
     2341 ttcatgttta taatttcaat attagatttt ttaaatttac cttcaatata ttctatggaa
     2401 tcagtgctgt tagaaatttt aagagaaact tgctctgctg tagggagttt catccatttc
     2461 ctatctctat gaagtgttat ctgtacaata acaataatga tacttgaaac aataccaact
     2521 aagtataatc cagagccaat ggcaagacct gtaccggctg ttgcccatat accggcagct
     2581 gtagtaagac cacttactga ttgatttcta ataaatatta atccagcacc taaaaaacct
     2641 atcccactga caacttgtgc tgcaacgcgg gctgggtcta gaccaatacc tggagtaccc
     2701 actatatcat taaatccata cttggaaaca atgattataa gtgctgaagc taatgccact
     2761 ataaaatgag tgcgtattcc agcttctttt agtctattct ttctctcata tccaattaaa
     2821 gccccacaga aaccagcagt aatgattctt gctatatatt caaattgaat caatatatga
     2881 tggttcattt atctagcacc cccttctata taatattata tatactataa tactacattt
     2941 tttatgtgaa tatagtaaag cttaaaattt atataatagt tatatatgaa tataaaatgt
     3001 ataaacgtat attatgtata taaaaatgat agaaagcatc taattttaaa tgattaaaga
     3061 gcaggcttaa ataaatcaaa cttgctcttt taacatagtg tatgttttta tttgtaatag
     3121 ttagataaaa atttatctgt gttttttaca tctgcaaaac tgttctgtag tgatgccatg
     3181 aatacttgat gtacagtatt tgcattaatt tctatattat tccaagataa attcttcgtt
     3241 gtacatgcat cgcttattag agttatgtcg tatcgaagtt tctttgctgt tcttacactt
     3301 gtatcaatac acatatggct catcattcca cagattacta gattagtaat attttttttc
     3361 tcaaggatac tttgcaaatc tgtttcaaag aaactatctg gtgtgtgttt agttataatt
     3421 tcttcattat ctagtggaaa aacaatttta tttatttctg aaccatgagt attaggaaga
     3481 aaaaaagaag ctgttttatc tgttgatata tgctgtatat gaataattgg taaattgttt
     3541 tttctaaaaa acaatagtat ttttttagca ttttcagcag tttcttctga ttgaaataat
     3601 tcacattttc catttttaaa ataatcattt tgaatatcaa tcaatattaa agctatagac
     3661 atgattatac ctcctattta tttttattat aatactattg taaaaaatgt aaattactta
     3721 ctttttggta tgtataggat tacttattgt agcaaattcc tttattatct agaatttctt
     3781 gttgaccatg ttcaagcata tattcgattc ctatttcttg cattatttcc actgctttta
     3841 acattttttt accacgatta gtaagaaaat attcaacatg taatggatat ccagcagaac
     3901 ttattttatc aataatccca aaatcacgta actcttttaa ttgttgaatc aacatttttt
     3961 gatttattcc ataaatatta tgttgcaatt cagaaaatgt tttattagat tgtcttaatt
     4021 caaatataag gatagttttc cattttcctt tgattatgtc atgtacaatt tcaagaggac
     4081 atgtatattc agctcttatt ttcatagcat atcaccccca gtaaaatgat aataaattat
     4141 atcacttgtc ttgtgatatg acaaatatat atgaactgta tataaatttt atgttttatt
     4201 aagttctaaa tttcttctat tgtagttcat atttagtaca taaataatta gtaagatata
     4261 agtaaataaa tatataacat gactatagaa atttaacttt aaaataactt atgataattt
     4321 agaggaggaa tatatatgaa tatatcaaga aaagctatga aaataataga attagcacaa
     4381 aaaattgcaa ataagagagg tatttcagta gaagaagctt ggagtgaagc agtaacagaa
     4441 tataaaaata aatacgaaca tattgcatag atatttaaaa atttagaata tttggtagaa
     4501 tataatggtt ttaaatgata ctataaatta taaggtatat cattattaac atagaaggag
     4561 gatatatgct tgattgtaaa aatagataat actttggaaa aagaattttg gcaatatgta
     4621 tcacatgaag aaagtttaaa tttattcata attggatacg ttgagaatta tggatttagc
     4681 tctcaatatc aagatatatg gtttcaggtg gaagatggaa atataacctc tataatatta
     4741 aaaaataagt caacacttat aatttatagt tttaaaaata attttgatat aggagaaatg
     4801 aagaatcata taaaagattt agatgtagaa agcataagtg gaaaaaaatg tgtaatagat
     4861 agactaataa gtaaatacaa agatttttat gaaaaactag ataataaatt ttgtgtatta
     4921 aaagaaataa aagaaataga tttttccaac atgaaggagt ataaaataga aaatgctcag
     4981 gaaaaagata ttgatgaaat aggaaaactt ttaaataggt cagactataa agtaagtaaa
     5041 aattacatag aagaaaggaa agaacattta aaagagggaa atgtaagagc atattttatt
     5101 agaaatgatg atactatgat tagcactgtg tcaactggta tagaaactag ttttttggca
     5161 atggtggtct ctgtaagtac agataaaaga tataggggaa aaggacttgc aagttatatg
     5221 gtttacaatt tgagtaaaga attattgttg gaaggaaaag ttccatgtct tttttataac
     5281 aatgatatag caggtaaaat atatcataat ataggatata aagaggtaaa tgaatggaca
     5341 attctcttta aataactata aaaaataaaa aactctatcg agaattaaag tatttataaa
     5401 taactatatt tttactataa ttcatgatag agttttttta atttatgata ctatttttta
     5461 ttatttaaat aatcaagtgt tttaagttta taaccttctt tttcccaatg tgtaagaacc
     5521 tcatctaata tctcagtatt tgttgatgaa tttggatgca gtaaaactat tgcaccagga
     5581 tgagttcgtg aataaatttt gtttttggca aattcatgag taggttgttt cttttcatac
     5641 caatctacat aagcaaaact ccaaaatatt gatttatatc ctaaatcttg tgtatatttt
     5701 aaagattgtt cgctaaattt acccattgga ggtctaaaat acttaggcat ttcttttcca
     5761 gtaacatctt tgtatgcttt ttctacactt gtaatttctt ctttgaattt ttcaaaatct
     5821 gtaatacctg ccattgatgg atggcttttt gaatgattac aaactaaatg tccttctttt
     5881 tccatacgct ttattaaatc tggattgctt tttatataac tttcaactac gaaaaattgc
     5941 gctttagcat tgtgtttttt aagcgtatct aataattttg gagtgtttcc actttcatat
     6001 ccagcatcaa atgatagata tataactttt tcattgggat ttcctacata ataagctccg
     6061 tattttttaa aaaaatcggc ttcttttata ggtgatggct gtttaccatc ctccctaggg
     6121 ttaaaatacc aatcatattc atgtgtgtcg agtgttggac tagaaatttg agtttttgtt
     6181 ttatctaatg atttaaaatt tatactggca attccaaaaa gtattagagc aaatgctcct
     6241 atcataatat atttttttag gctatctttt ttcacaattt cacctcttat ttaaaattta
     6301 aagtatattt cttataatat agtttgaata tttatagtta aaaatattca catatacaaa
     6361 taataaatga aagaagttta ctgtaagata taaagttgat attaattatc ttaaataaag
     6421 tgtatataaa tgataaaata taaagagaaa tgattgtaaa taagttaggc tgatttaact
     6481 tgaagggata taaaaatgga taatataata agtgtattga atgatgttgt ttttttagat
     6541 atagaagtta ctggattaga ttgtttaaat tcagaaattt tagaagttgg agctgtcaag
     6601 gttaaagatt ggaaagtata tacatatgaa tcattaataa aaaacaaatt tgaggtacct
     6661 atagaagtat tctcagtatg tagaaattta gataaaaatg atttagaaat agctaatgaa
     6721 atagaaatgg tagaagatag acttgtaaat tttgtagaag ataacttcat tatatgtcat
     6781 gatttaagtc tgaaaaaaaa gttttttgag tatcatatgc caaagttaaa aaataaattt
     6841 attgatttaa ttgaattagc agttatttta gaaccatatc acaaagatta tagcttagaa
     6901 tacttaaaaa atactttaac aaattgtaat tctaaagtgg aaaatagagc tttatcagat
     6961 gctattgata taataaatat tgtaaattgc cttttagtaa aatttaataa ctatgaaaaa
     7021 actactttgg agccattgag ttttaaaata aattcatatc ttaaaaaatt taatcttcca
     7081 acatgggaat ggagcagatt tttagaagag gcaaattatg atttatcaaa taatataaat
     7141 atcaaaaaag aatataatat atttgactca aaagaagaaa agaagaagga aagagaaacc
     7201 ttaaaaattt taaatgaaga agaaaaaaat tatgaagaat tattaaaata caaaactatt
     7261 tgggagaata aagaaggatt tacatatgag tatagacctg gtcaatatga gcttactaaa
     7321 acaataagag agttgtttag aaatagcgaa gatgaagaaa aaattgcatg tatagaggca
     7381 ccaactggta taggtaaaag tgtaggatat ttattgcctg caatattaga ggctagaatt
     7441 aataaaaaaa gattattaat ttctactgat acaaaggaat tgcagataca attaataaat
     7501 aaagatattc caaatgtgtt agattctttg ggtttaaatg gaaaagtaag ttacggatat
     7561 ataaagggaa aaaataacta tatttgtata gatagactgg aagcatatat agatgattat
     7621 gagtctcaaa accctactaa aggagaactt ttatctttaa tatttttgaa gaggttagtt
     7681 gaaggtggca agtatggaga tatagaagaa ataaattatt cggtgtttga caacttcaag
     7741 gaaataagta ctcacttaag aaatgtaagt tgtgacccaa atatgtgcag acctaaaaaa
     7801 tgcaaaaaag attgtttata taaaaacaga atagaagaat tgaaagaaga acatataaca
     7861 gtagtaaatc attcactatt agctaaatgg ccgtataagg atgaaaaacc tttagaaaat
     7921 ataatagtag atgaagcaca taatttgact gaaaaaggat atgatttctt ttcaagcatt
     7981 ataaattcaa agtctttaag gtatttatta caagaaattt atccttacga gtttattcaa
     8041 aatagtagct ttatatataa aaaatatagt agaaatatga gaaaaataaa ggcttttgat
     8101 aaattttata atgtattaaa aattgaaaga gaagataaac aaaaaattgc tagaagcata
     8161 aatctcatta ttgaagagat agattcaata ttaaattttg gtaattgtaa tgaatataat
     8221 aatgtaagta attataattt aagatgggaa ctgaacttgc aaatagatga aatcgtggga
     8281 aaactaaaaa aggatggtgt agatactgaa ataagttata gagcttatag tgaaaagata
     8341 aagttgagtt gtgagaaaat aataaaaaat ttagtttcaa taataataat aatttatagg
     8401 aatattgatg atgatagtat agataaagaa gcagatatat ataaatttgg aaaggcaaag
     8461 acaagagatt tagaggatat aaagattata tttgaaatct ttttggagta tgatgaaaaa
     8521 gatgattatg caaggatagt tgaaattgat aagaattata atgactttga gtttcgagta
     8581 gtgccattaa aaatagcaga tttatttgaa gaaaatattt tatctcaatt agaaaaagga
     8641 atatttttat ctgcaacttt gagtttatct gaaagtatgt cttattttaa aaatacatta
     8701 ggaatagaca gagttaagaa tgtggaaaag attatagaac caatttttga ttataaaaat
     8761 agagtctctg tagttggatt ttctgatatt tgcgagtaca gaaattctga atttccaaat
     8821 gaaatgagca aaattatatc taatatatca aagattacag aaggtcatac attagctctt
     8881 tttaatagta aagatagaca agaaaaaact tatgaaatat tgaaaaaata cttacatagt
     8941 tttaacttag aaatttatgc tgataaaaag ggcattagac accttaatga tttaaataga
     9001 aaatgtgtag tacttggctc aaaaggatgt tttgaaggcg tagacattcc aggggatggt
     9061 ttggtttgtg taactttaga caagcttcca aatctaaatc caaaagaccc tttgtatttt
     9121 actataatga agaagtatag catagactac tatactataa attatcctca aatgaccata
     9181 aaggtaaagc aagctatggg gagaatttta agaagcaaat atgactatgg atgttttgtg
     9241 atttttgatg taggaactaa tctaagtgtg ttaaagagat tagagaaaga tttacacgat
     9301 tgtaaaattt caaaggttaa tagcaatgag ttttatactt atataaggcg acatttaaat
     9361 aaatctagaa gtttaatact aaaatctgtt atatttgata ctataaaagc gttgaatgtt
     9421 gatgctaaaa tggataacaa tgatgttgat aaggatatta taaaaaaaga tataaatgaa
     9481 aatataagac aaagagctgt aaaaggagaa gtgtaccata ttgatattat aaaaaaagat
     9541 atgaaagtaa aatattttga tagaaactat cttataaatt tagatatatt tatgagagaa
     9601 gaagataaaa attaagagtg ggagtgatat gtttggcaaa acaaaagttt tatgctgtga
     9661 aaaaaggtaa aaatataggt gtatataata catgggatga gtgcaaaaaa caagtaaatg
     9721 gattctcagg agctgaatat aagagttttt ctacttttca agaggctaaa gaatatattt
     9781 atgggtcaga aaaattgagt tttcaagaag ataaggagtt tatagaagca tatgtagatg
     9841 gaagttatga acattcaata aaaatgtatg gttcgggtgt tgtgatttta aaaaataatg
     9901 aagttataaa aacttatagt gaaaaaggaa aagaggaatc tttagtaagt atgagaaatg
     9961 tagctggaga gatagaagct tctaaaattg caatgcagta ctgtattgac aataatgttc
    10021 aaaacttgat tttatacttt gactatgaag ggattgaaaa gtggtgtact ggagtttgga
    10081 aaacaaataa aaaaggaact atagcatata aaaatttcta tgacagtatt aagaataagc
    10141 ttaatgtaaa atttacaaag gttaaagctc attctggaaa taagtataat gaggaagctg
    10201 ataaattagc caaaaaagct attggtgtat aataaaaaaa tatttataca taattacata
    10261 gtataagagt atatctaaat tcctacttta taattaaaca acttataatt aaataacttg
    10321 taattaaata aattttgatt aaaaaagcag attgtattat gaaaaaataa gtatctaatt
    10381 tataaacaat taataatatt attaattagg tacaacttat ttaatataat aaaatctgct
    10441 ttttatataa ctataaaatt ttcctaaatt ctattagaat tgcccataaa taccttcaag
    10501 ttcttctttt aatacttcag ggtcatttat accaatagac atggttctag ccagtatatt
    10561 tgcttggtcg taagttgacg tccatgagaa tacaccacct agacaatttc taagaacata
    10621 ttgagttttt aagaatattg ataaagcatc atcatatgac attgcaaaat ctccatcttt
    10681 aactaaatac ggaacttgag cagtattatc aaatctatat tcatatccat ttttatttat
    10741 ataatccctt ctaagttcat cataagttct tgttatagta gcacctaatc ttccataaaa
    10801 tggtatacca agaaggattt tttcagaagg cattccagca ttctcaagat ttctcaccat
    10861 tgcatcaaca ctatatcctg gcaaagataa gtctgaatca aaaagatttg cttgatgttt
    10921 tctaccattt ggacctgttt cacctgctgt aaaatcataa ctcataagat taaaataatc
    10981 tattatagga gctattttat ctatttcagc acttgaattt atatatcctc tatctcctgt
    11041 tccagctaca ctaagccatt tatcatctcc tataacatct cttatggcag ttagtaagag
    11101 tgtgaagttt tctctatctt gaggtcttga tgttattcca gatgcactac ttccaggata
    11161 ttcccagtct atatctattc catctaaagc atattcattt atcatctgat tgacctgtct
    11221 tgcaaaatta tatctagatg taggcgttaa tgctgcatca gaaaaacctt cagctcccca
    11281 acctccaata gcgacaatta cttttaagct aggtttttca cctttaagag caagtaattg
    11341 atttaaaaat ctaggataag gaataaacaa atttccagac tggtctatct cagcaaaagc
    11401 aaatataact gcatctaata gttgagcatc tacattagtt ggatttccaa gaacatattc
    11461 tgctacaatt ggatttatat ctggacagtt tgcattttca actattggat tgtatgggtc
    11521 tgttatttgt ggtctaatta atttttttga atcttttaaa gtcttcatag ctattttttt
    11581 agtatttttt tcaccagtat aatcgtagtt tttataatta tgtttgtaga aatcttcata
    11641 gctactatct tctttgtcat agctagaatt tattttgtca caagattcat ctttgtcata
    11701 gttaaaattc atttcatcac aagacttatc tttaaactta tcttgtttgg agttgtgaac
    11761 cttgttagta tttttagtat gttttttctc atggttgcaa tcatttttaa tatcagtttt
    11821 gttttcttgt ttacagtcaa gagctttgtt caggtagtca tgatgttcat gttcacagtt
    11881 acattcattt tcaatgtttt tagtatgttc ttttttccag ctacaagtat tatcaatttt
    11941 cttgttcact tcttcatcat tataattatc tggtacaaaa cataagtacc agtccataca
    12001 ggaatatttc ttattacaac tgttaactct attttttaat tccttatagt ttttaggata
    12061 aggtaaaatc actggcattc caggaaacca gtttgcagga gtaactatat tatctctatc
    12121 actagtctga agtgcatcta ctattctaag tatttctgga atatttcttc ctgtagttag
    12181 tggataataa agaatcgttc ttagaatttg attattatct ataataaata cagagcgaac
    12241 agttgatgta tcactcattg gttttgatat cataccatat aacttggcaa ttctcatatc
    12301 tctatcttct ataataggaa atggaatttc tacacctgta agtaaagaaa tattatacat
    12361 ccaagctaaa tgtgaactgt tactatcaac acttagacca attagttctg tatttctttt
    12421 tttaaattcg tcataatatt tagcaaaaca taaaaattct gtagtacaaa ctggtgtaaa
    12481 atcaccagga tgtgaaaata aaacaatcca attaccctta tagtcagaga gtctaatagg
    12541 gccatttgtt gtattggctt taaaatcagg agcctttgac cctaaacttg gcaaatttgg
    12601 catgtaaatc acccctcaat aaattatcat ttattttatt ctattaatta atacatatta
    12661 gtgttactac tttattaaaa gttaagtaat ccttatgtca aaaaaagata tttattaaaa
    12721 agatttaata ttttcctaat ataaatgaca atagtaattg ctattttttt actaatttgg
    12781 tatagtaatg gataaggggt agtgtcaatt ttagtgatta tacgaggtga gtggattgaa
    12841 aaaaattcta agaattttag ttatctccat gatgatatgt tttgcaatga cttttgcatt
    12901 attgttaata aattcagtta tgggagtaca tataggattg gattattact ttttgatggg
    12961 aagcgtgctt acagtttgtt taacaattgg aattgtttta tgcaatgcta gaaaaatatt
    13021 tgatagcaaa cctatacata aaacaagaac gaaaactgtc agtactaaca aaaaacaaaa
    13081 gagtcattct agggatataa aaagaaagat atcataattt atgtaaagga aatatttatg
    13141 tataaaataa tgattgatag gtaaaataaa aacacgattt aacttgtgtt tttatttttt
    13201 ttcaagaatt atgatataat aaccaataag aaatttagtt taccataaaa tataaagatt
    13261 attaaatgga gagggaacta taatgaattt tagagattta tttgagaagg ctgtagattt
    13321 tattgatgaa aaaaggaaat ctatagttgc gatttcacta tctatattag gtgtaattgt
    13381 tttgataata gtatttttct taagtagtaa cgagtttagt gtaggaaatg aagctaatga
    13441 gttgttaaag attatagaga aaagacagta ttctatagct gttgattact acacaagtat
    13501 agaaaaaaag ttttctgatt caaaaatgga aaggtttaat aagtctgttt ctaaaaaaat
    13561 aaataaatta ctattaaata gtggtgataa atatttagat ggagatataa gtcaagaaag
    13621 ttttattgga ttaataaata ccgtgaaaga attaaaaaaa ataagtatag atgtggatga
    13681 cttattaact caagctgata gagttagaga aatgtataaa gaagaaaata ctacttatga
    13741 tattgccatt aactacataa atactgtttc aatattaaat ggcataggta gtaacctaga
    13801 tgtatacaaa cagaatatag aaacaatcaa agagtctaga gatgtttatg actcagcagt
    13861 aaaagaccaa aaagcatata agtattatga agctattgaa aagtataata aagtattaaa
    13921 agaagatgaa aagtattata gtatggctca aaatgggaaa gaacaatgta ttgaagaaat
    13981 gtatgattac tatataagta gagctgaaga ggcaaatact tctggagatt atgaaagagc
    14041 attacaatat attgagtatt taaaagaaga ttattcagat gatgaaaagg tacagagttt
    14101 agaaaaaaaa tataaaaaga atttatcatt gtatacttta acatctgatg acattataaa
    14161 tgtaatagct aaaaaaagtg gcaaagacaa ggcaaatcta tctataaatt cacttccaca
    14221 aatgataaaa aataataaat actattatgc tgaagtatat gagtataata aattaataaa
    14281 tgaagttttg gtaagtgcaa aaactaaaga tttatattca tacaaagatg gtaaaaaaga
    14341 ttacaaagta gattatggtg atggatactt tagaatatta gaagatggaa gttatcaatt
    14401 tggtatcaca aaagataaag ctaagtttgt gttaactaat actcttgatg aaaaagaaaa
    14461 taagtataaa aagattgaaa tattagatat agagaaagca gatagatatg taaaaagtaa
    14521 aaagagttta gaagaattgt ttggaaaata taaaaatatt tattattatg ctgtagtaaa
    14581 taaaggctta tttagaggca aagaagttta tgctattaat atttacaatg aaaaaatata
    14641 tgccatatca gaaaatggac taaatgaata ctaattaata atattaaatt aatatataat
    14701 taaaaagttt ttggggtttt aaggcgcatt atgtagtaat ataataatag ggatatataa
    14761 ataattttat tgtaaggaga agtataggat gagtaatgtg aacaaaaagc tagttatagc
    14821 tattggagct gtagcattag tagtaataat ttttataggc ataatgtgca tacaatttaa
    14881 aggggagaaa atagcaaaaa acacatatgt taatggagta aatattggaa aattaactaa
    14941 atcgcaagca aaacaggaat tagctaaaaa atacaaatta gagaatgttg agtttaatta
    15001 caatgataaa agttggaaag taaagagcaa agatttaaat ttatctactg acctggataa
    15061 aacagtagaa aacgcatata atttaaatag aaagagtggt ttctttggaa atctatcaaa
    15121 aactatttca gcaaactttg gcaaaaaaag caacttagta gttgtgataa attatgataa
    15181 aaataaatta aaagcagaaa tggaaaaaat agctaaagaa atagatgtag atgtaaaaga
    15241 tgctacactt gatataagtg gtgaaaaagt taaagttata ccagattctg atggtcttaa
    15301 aatggatatt tctaagagta tggagaattt tgataatcaa actaagaaag gtaactataa
    15361 aaatgagtta gtagtaaaag ctactccagc aaaagtaaaa aaagagcaac ttgctaatat
    15421 agatacaaat ttaggtacgt attcaacaac atttaaaact tctcaaataa atagaagtat
    15481 aaacataaaa ttagcaacag ataatataag taatgtatta cttatgccag gagaaacttt
    15541 ctcatttaat aaacatacag ggaaaagaag taaggaaaat ggatataaaa gtgcacctgt
    15601 aataatggaa ggtgaaatgg aagaagatta tggtggagga gtttgtcaag tttcatctac
    15661 attatacaat tctgtgttat acgcaggttt agaaattgtt aatgtaaaaa atcatactat
    15721 accatcaagt tatgttccaa aaggaagaga tgcaactgtt gcagatagtg gaatagattt
    15781 cttatttaaa aataatctaa agcatccagt ttacataaaa aattacgttt caggaaatca
    15841 aattgtttgc aatatatatg gaagtgcaga agataaacaa aatataacaa tatcaacaaa
    15901 attagatggt gtttcacaaa ctacaatgaa aagagttaat gaccctacaa tgcctaaagg
    15961 taaagaaaaa gtagacaaaa gtggaagaaa tgcatattct gtatcaacat atagaacatt
    16021 taacgatgca aatggtaaaa aaattaaaac tgaaaaaata gctaattcat attatcctaa
    16081 gaaggaaggt attatattag ttgggactat ggaaccaaaa ccagaggaaa aaccaaatat
    16141 agacgaaaat aagaataatc aaaatacaaa caatcaaaat acaaataatc aacaaaaacc
    16201 agaaacacca ccaactgata ataagcctaa tgagacacaa ccacaaccgc aagcttaaag
    16261 ttctcaagaa taggatttat gtagagtaat aaaaaatata aatctaaata ggagggatta
    16321 ctttgaattt aaataatata acgaattacg ttcaaagtat ttgcgagaat atttctaatg
    16381 tcttggatgt agatgttact ttagttacaa aagatttaac taggatagca ggtacaggaa
    16441 tattcagagg taaaatagga gagacgatat cagagaagtc agtgtactct cttgttttaa
    16501 aagatggaaa atcctatgtt ataaataagg aaatagaaga gaactgtaat aaatgtgcaa
    16561 atagagaaaa ttgtaaagag ttagctgata tatgtactcc cgttaaaatg ggagagcata
    16621 tacttggaat cttgggtata gctgcgttta atgaagagca aaaagaaaag atactttcaa
    16681 aagataagga gttaactgct tttgtaagga gtatgtctga tttaatatct tttaaactag
    16741 atgaacttta taaacaagaa gttaagacta tagatgacct tgagagggaa gctatagaaa
    16801 atgccataaa gaaatatgga agcaatacag aaggtatgaa aaaagtagct gaggcattaa
    16861 atataggtat agctacactc tatagaaaag taaaaaaatt caacattaaa agttaatagt
    16921 attgaaaaag aggtaacctt tttatgaaaa gaagaggatt acctttttta ttatgagaat
    16981 ggtggaaaaa tatgtatagt tttgacttga catattaaat atgttatctt aaatatatat
    17041 tttagagtgg aggtgagtat tattttagtt aaagatataa aacaagatat agaaagcatg
    17101 cctagaaaaa ttttagatat atctttgata gaagataata aaagtgattt gacttttttt
    17161 aatatagaag aagaagatgg tgatgaaaca ttaaaagcat taaaacagag ttatgtgact
    17221 acaaattata aagaagaaac tctattaaac ttgcagggag ctttaaattc aaagtcggat
    17281 acaacgtcta aggagttaaa attagaatca tataaaagat ataacaatgc attggattta
    17341 gcatataaaa attatataac tagtgccatt aatatggtta ataaagctct ggagataaat
    17401 cctaaagaca ttgatatact aaatcttaga ggtcttttaa agttattaaa atgtgatttt
    17461 gctaaatcat ttgagagttt ttatactgct ttatgttatg aaaataatca tatatctaga
    17521 aaatatgtaa atctaatttc atctgaagat tttaaaactt ttttaggaag atacaatcat
    17581 tctataagat ttataaatga agaattaaat tatgagtcaa tacatatttt agagaacata
    17641 attgaagaag agcctgagct aatagagcca tatgtaatct tatctttgtt atatgataaa
    17701 ctaggtaatg taaaaaagag agaaggatat ttagacaaac ttaaagagct agataaagat
    17761 aatcctgtat ttgaaaaaaa tgaagaagaa aaagaagaca caagcaaaaa ggaagaaata
    17821 aaaaaagtaa aaagaaagaa aagtatttta ccatatatta tagttggctg tgttttaata
    17881 ggtatgggga tatatttgat tcaaagcaaa aagagaatag aaaacttaaa taaccaaata
    17941 agtagtaaag aggaaaagtt aagtgaaact gataaaaagt taggagaaac gagtaaaaaa
    18001 ttagagaaaa cgaataagga attaaatgaa gttaaaaata aagaaccaaa aagagagata
    18061 actgtagctg atgaggaaga tttatattat caagctttag atttaaagaa aaacaaagaa
    18121 tatgaaaaag ccatagataa ttttaaaagt gtagtttctt ctggtaaaac taaaaaatat
    18181 atttctgaat caatatatca attagcaata acaaataaat tattaggaaa taaggatgaa
    18241 gctataaaat attacaaaaa atatataaat acttatacta aaaatgacca gtattatgat
    18301 gattcatatt acgagttggg tatgctttat tatgataatg gcgatttaaa aaatgcacaa
    18361 cagacttttt acagtttgag aagtgaagtt ccagacagta tgtataacaa ttcaaaaata
    18421 aaagaaattt taagtgaaaa ataggtttat atagttaggt tttttgaaac aaccaagtat
    18481 gtattttatt aaattaaaat aaaaataaat ttaaaaagag attgtgtcaa attataactt
    18541 tctattttaa agaaagatat ttgcttgttg ataaaactac aaatatatga tttgaagcaa
    18601 tctctttaat ttttggaggt ggaaaatgaa agttttcaat caattagtaa taattttatc
    18661 tatctgggta ataggagagt atataagttc ctttatacaa ggaattatac ttattcctgg
    18721 gagtatagtt ggtatgctat tattatttct actactacaa tttaaagttt tagatttgag
    18781 tagtatagaa aatgtaagtg gatttttcct cgataatatg gctatatttt ttattccagc
    18841 aggtgtatca cttataaagt cattagacct aataagggaa aatgtattag tgcttttatt
    18901 agttatatgt ttaagtacac taatagtaat gtatactact ggaattatcg tggagaaaat
    18961 gataaaaaag aaggaagaag gacaaaaaaa tgtataatgt attacagaca ccagtatttg
    19021 gtataatagt aacaatagtt tttttcaatt taggaagata tattcaacga aaaacaagta
    19081 atcctatatg taacccttta ttaattgcaa tcgtaggaat aatattgttt ttaagcatat
    19141 ctaaaatccc ttatgagaat tacaagatag gagctgatag tctaaatttc tttttaggtc
    19201 ctgtcacagt agtacttgca gttccacttt ataaacaatt tgaattattt aaaaagcata
    19261 tgtttgagat tattgtagga ataggttgtg gtatagtgat ttcttttgta tctgtattga
    19321 taattggcaa aatagctaat tcagatgtaa gtataataaa ttcactaatt ccaaaatcta
    19381 taacaacacc aatgggaata tctttagcaa attctttaaa tggtatagaa tcaataacag
    19441 ttgtagctat aatttttaca ggtatttttg gcggtatggt tgcttctaca gtattcaaac
    19501 ttgggaatat aaatcatcca gttgccaaag ggattgctct tggaacatct gcacatgctt
    19561 taggaactac aaaagctttt gaacttggag aagtagaagg agctatgagt ggtgtttcta
    19621 ttggagtttc tggtacaatt acagtaatac taattccaat tataatgaat tttatataaa
    19681 aaataaaatt aagacaagta ccttgaatac ttaaaacaaa ataaaataaa aaaaatttaa
    19741 aataaaaact gaacgaaaaa aatgcaaata atgaaataaa aaaatcatgt actgaaatta
    19801 taaaaagaaa aatttcattt aggaattaat tataaagaga taattactaa atttgaattt
    19861 gctaaaataa aataaattac tgctaaaatg gatatttaga aaaaaatact ataaatttac
    19921 tatattgcaa gttaaataat aagcagtatc atattatgct agaaaaagtt tcattcaaag
    19981 cataaatgag atgatttgaa cgttttttaa gattagaata taaaagattg cactagacat
    20041 atatgaaaaa gataatataa tagatgatgt aatagatgac agttcaagaa attatataaa
    20101 aaaatttatt tgaaatatta tctattacat caggaataat atatgtaaga gtaagctttt
    20161 aaaattctaa agatttagaa agattgaagg gagaaagaaa tgagtgaagt taagaatacc
    20221 ctacaaaaac atccagtttt aagatattca acaatgctat ttgggatgtt gatgacatct
    20281 ataggaataa atgggttctt aagaccagca catctgctta gtggaggtgc tacaggtata
    20341 gcaacatcta taaactattt gacaaatata aatgtaggtt tattaacatt tttaatcaat
    20401 attccaatat ttatcttagg gttcatatac cttgaaaaag agttttgtat ttcaagtttg
    20461 gtaaatatga ttgtattttc attattatta ggagcaactc aggaaataag taatattata
    20521 cctatacatg atatattgct tcaaagtgta tatggtggta tattatctgg tttaggagtt
    20581 ggagttgtat ttagaactag gtcttctcaa ggtggtacag atataatagc tgcaatacta
    20641 aaaattaaga agaatataga aatgaaagac actgctttag caataaatgg cttgatagta
    20701 cttacaggaa gttttttatt tggattggat ttagcattat acactcttat aggtctattt
    20761 ctaaatgcgt attcaatgag ttttattaaa gatgcaatga attaccaaaa atctgttatg
    20821 gtaatgtcaa atgaagtcga tttaatagca gaagatataa tgaagagttt agttagaggt
    20881 gtaacttttt tagatgcaga aggtgcatac actcatcaaa aaaagaagat tatatataca
    20941 atagtttcat caaatgagat accaaagata aaagatatag ctttgaagta tgataaaaag
    21001 gcctttatat ctgtaaatga tgttacagaa gtaaagggaa gaggatttaa agcaaaagat
    21061 ttatagaaaa ttttacaagc tgggagttta taatgtaaat ttgtatatta tatctctcag
    21121 cttatttaaa tttagtatat gaaaataaag gttttattaa tgatttgtgt atcactttaa
    21181 aagatatcat tttaaaatta aaattgaaaa aattacttaa tatgtagaaa acatgaccac
    21241 tattaggata taataaattt aatagattta gtaaaatatc taggaataac gttggaggaa
    21301 tggcattatg aatttatcta aagaatatgt taatttgtat gataattatt tgaaatatat
    21361 aaatgaagta aacaaagaga ttagaagtat aaagaacatg aagaaacgtg ttttaaaaag
    21421 gggcgatttt gccccagaaa atgaagtatt agaattggaa ggaaaaacaa tacgcgatat
    21481 agatgatgtc gatacaaaaa aaccaaaaaa taaaatctat aaattttcaa atggggatgt
    21541 atatatagga aaattcttag agggaaagat ggagggaaaa ggctcatata ctttttttgt
    21601 cgatgaagga acagttatgg agtacatagg agagtttaaa gaagataaaa aaaatggaaa
    21661 aggaaacttt gtattttcaa atggaaatga atatgtaggt tattttgagg aagatgtgat
    21721 gagtggtatt ggaaatatgt tatataatag caaagatgaa tatattggaa cttggaaaaa
    21781 cggaaaaaaa gatggaaaag gtatttataa gtggagtgat ggatgtatat atgcaggtga
    21841 atttaaaggt ggaaagatgg aagggtacgg tatttgctat aatagtaaag gggaagtact
    21901 ttatgaaggt gaatggaaga ataatttgat tcatggtaag ggaacttata tatgggaaaa
    21961 aggtaaaaag tatataggtg aatttatgca tggtaaaaaa catgggcaag gaacttttta
    22021 tctaaataat gaattggttt atgaaggtac ttggaaattt gacaagccat ctatatttga
    22081 tagaacatta gatgaaatat tttccttgag actgtagctt tatctaatta gtagataaag
    22141 cttttttcta atacttactg taaataggta tgaatgatat tgattagtat acactatcaa
    22201 tattaactta ataaatgcaa tattaaaaaa ttttgattgc ttttaagaag gagagatata
    22261 ttgaagagat taattgcttt agtgttagta gtgcttattg ttttatttta tagtcctgaa
    22321 ttattaaaag aaggatatga tttaaaagaa aagttttttc agagtgaaat atggaatgaa
    22381 tttttagata aaataaatat aaatgataat tctaagaaag ataaaaaaga aaaaagtcaa
    22441 aatttacaat caaataatac tgaaggggag aatttaggag agtcagacat aaagaatttt
    22501 gaaaaaataa gcttaggaga caatttaagc tatgttttat caagtattgg taaacctggc
    22561 agaattgata ttagtgaata cggttttgat tggtatgtat ataatcaata tggaaaagaa
    22621 tttgcaatgg ttggacttga aaatgatgaa gtagtagcac tttatagtaa ctctattaat
    22681 tcatgtgaaa atcaagatat aaaaataaat caagatagac aaacagtgag aactaagatt
    22741 acaccattaa aatataagag aaaaggaaat accagatata taataaattc agaaaatcaa
    22801 tatgatataa taagtaaaga aggtaagtat attacaattt tttacgatat tcatgaagaa
    22861 aatagagttt gttcatattt aataattgat aaaagtactg aagatgagtt taaaaattta
    22921 tatccagatg atagcgaaga acttaaaaaa tgttttgaac ttgaaattat agaccttgta
    22981 aatagtgtta gaaatcaaag agggttaaac tcattgagat acagtgaaca ggcaactttg
    23041 agttcaagaa aacatagtga agatatgaga gataataatt tttttgacca tgtaaataag
    23101 aagaatgaaa ctccatttga cagaatgaaa agagaaggga tagtatatac ttcagctgga
    23161 gaaaacatag cagcaggaca gattaatgca atatatgcac acgaagcttg gatgaactct
    23221 gaaggacata gaaaaaatat tttggggaat tataataata ttggagttgg agtaatattt
    23281 ggaggaagct ataaaacata ttacactcaa aatttttata aataactaaa atatttgaaa
    23341 ggaagttaaa aaatgacatc atggaaaaaa aagacagtat ataagtgttt aatagcagta
    23401 gtcttgtttt gtggaatagt gttaatatct aattttagca aagtttctgc tttgatgatg
    23461 gatactaatg gaaatgtttt gattaaacat ggttcaaggg aaaaaaagct aatagcaatt
    23521 acctttgatg atggtcctca tcctaaagaa actagtcaag ttttagatgt gttaaagaaa
    23581 tacaatgtaa aagctacttt ttttatagct ggaaaacatg caaaatggta taaagaacca
    23641 ttagtaagag caagtaaaga aggtcacgag ataggaaatc atactttcaa tcatcctgat
    23701 ataagtaatt taagttctag tcagatagaa gaggagattg ttaagtgtga ggatattttg
    23761 aaggaagtaa ctgggaaaaa acctacatta tttagacctc catttggaag ttatagagaa
    23821 aaagacttaa tagaaattgc gaagaagcat gactataaag ttgttttatg gacaggtgta
    23881 gatgtgaaag attggaaaaa tccaggagct aatagcattg cagataaaat aattaataaa
    23941 gtacaaaatg gagatataat acttttacat gattatgcaa ctaatgatac agttgaagct
    24001 ttagatatgt ttatcccaaa aatgattgaa aaaggtttta aatttgtaac agtttctgag
    24061 ttgataaaat aatttttagg gggagattta cggtatgatt cttatgatag acaattatga
    24121 ttcttttgta tacaacctag tacaatatat agaagaatta ggggaaacag ttgtagtaaa
    24181 aagaaataat gaaatcaaaa taagtgacat tgaagaacta aatccagagg taattgtatt
    24241 atctccagga ccgtgttctc caaaagaagc tggtatttgt atagatgttg ttgagcattt
    24301 taaggggaaa aaacctatac taggaatttg tttaggtcac caaacgattg gacatgtttt
    24361 tggaggagat attataaaag cacaacagcc tgtgcatgga aaagtatata gcatcagcca
    24421 tactaataaa ggagttttta gtggacttaa aaatccttta aatgtcacta ggtatcattc
    24481 actaataatt gattccaata cagttccaaa agaactagaa attacagcaa ctactgataa
    24541 aggggaaata atggggatta ggcataagaa atatttaata gaaggagtac agtttcatcc
    24601 agaagctatt ttgtcagagt atggtcatga aatgcttaaa aattttataa cagaagctag
    24661 agaaagggta catatgtaat atgataagag agataaacac taaattaaat tcttttgaga
    24721 tatttactat ttttagaaat gaacatgaca gttttatatt agacagtgct atggataaag
    24781 aaaaattggg aagatattct tttataagta gccaaccatt taaggtatta aagtataagg
    24841 atactgatga aaatccttta gaagttttaa aagaagaact acataagtat agggttgcaa
    24901 atgatactaa tcttccattt gtaggaggtg ctgtgggata tttatcttat gatttaggta
    24961 attacataga aaatttacct agaactgctg tagatgatat tgagatgcct gatatgtatt
    25021 ttggatttta taatcatgta atagttatag accatcttgt gcaaaaaact tatatagcta
    25081 ctccaaacat agatatagaa ttagaagaaa agataatgga tgatatagaa caaagaatat
    25141 taaaagaaga gaaaaaaggt atagatagca tatgttatga agaaaaagaa gtaacaccta
    25201 taagacttaa atctaatttt acaaaggaag aatttaaaaa tgcagttcaa agtgttagag
    25261 agtacataag acaaggagat atatatcaag ctaatttaac acaaaggttc agtggagaaa
    25321 cagaacttac aagctttgaa ttgtataggg acttaagaag atttagccca gcaccatttg
    25381 gggcattctt aaactttgaa gatacacata tattatcaaa ttctccagaa agatttataa
    25441 aatgtgttaa taagagaata gaaactagac caataaaagg tactcgtcca agaggtaaag
    25501 ataaagaaga agatttaaaa cttcaacaag aacttagaaa tagtgaaaaa gatagagctg
    25561 aactacttat gatagtagat ttagaaagaa atgatattgg cagaatatct aaaactggaa
    25621 gtgttaaagt tccagaacta tttgtaattg aaccatatgc aaatgtaaat cacttagttt
    25681 ccacagttgt aggtgagtta aaagatgata aagatgctac tgatgtaata aaagcaactt
    25741 ttccaggggg gtctataaca ggagcaccta agattagagc aatggaaatt atagacgaat
    25801 tagagccaac gcaaagaaat gtatatactg gttcaatagg ttatataggt tttaatggtg
    25861 atatggattt taatatagca attagaacaa taatcaaaaa tgataaaaaa gtctattttc
    25921 aagttggagg aggaatgact tgggattctg acccagatga agaatatcaa gaaacacttg
    25981 ataaagcaaa atctataatg aaggctttga ggggatatta tgaagaataa tgtaggtttt
    26041 gatagtagtt taagtaaatt tggaattggt ttgtttgaaa ctattagagt taaaaatgga
    26101 atagcaatag atttaaatat ccatatagaa agaatgataa gttcaataaa ttgtttggat
    26161 ttagatataa actataaaaa agatttcttg ataaatgaaa ttgttacata tataaaaaaa
    26221 gaaaatgtca ttaataaagc acttagaatt actgtttttg atgaaggtta taatatatct
    26281 attagggata ttccatataa tcaagagacg tatgagaagg ggtttaaatt agctatatcg
    26341 ccaatagtta gggggaatag tttaattcat agacataaga ctactaatta ttttgaaaac
    26401 atatatacca aaaatatcgc aaataagact ggttataatg atggtatatt tttaaattca
    26461 gaaggtgtaa tattagaatg ttctatgagc aatatcttct ttataaaagg agaaagagtg
    26521 tgcactccta gtgatagatt accgatatta aatggaataa taaaaaaaag gattatagag
    26581 atttgtgacg aattacatat agagttgata gaaaatgaaa ttaatatatc agaaattagt
    26641 agttttgatt ttgtttttgt cacaaatagt cttatgggcg ctataaaggt tacagaaatt
    26701 gacaaaataa aatttaataa agaaaatgtt gtttttgata aaatagtgaa gttattagaa
    26761 taaattaaaa tattaacaat ttgttggaga caaaatatga taggttgtat ttatgaagta
    26821 tacaatgaag agatgactaa aataatagaa aaccaaaaca ctttggctgt atatttggtg
    26881 ggttctagta aagatgtaga ttttactctt tatgatgtag agataaacga tattgatgtg
    26941 ttcgtttttg taaaagaagg ggaaaaacaa gagagaatac tgtttaaaaa aaagggaata
    27001 gaatttgata taaattactt ttcaaaagat ggtgtaaaaa aattattatc aaatagggaa
    27061 tatttctttg tgaaagaaat gaaagatgct aaagtactgt ttgataggga gagtatatca
    27121 catattataa aagatataag tagaaatatt tacttagaag gtcctagtaa aatttcatta
    27181 gaggaaaagt cttttataaa gcaagatatt ggagcaaaaa tatcaaattt aaaaaataaa
    27241 gaaaaatttg atgtttttga atatcatttt ttgacgaatt tatatttaaa agatataata
    27301 attgggtact ttaatataaa tgataaatgg gtaccaaaag acaaaaaatt gctaaaagtt
    27361 ttaaaaaaag aaaataatga gctttttgaa ctagtgtcaa aagtaagtga aaattatgac
    27421 tatcaaagac ttttagatgt ttataattat atttttaaag aaattgagac aaaagaggtt
    27481 ataaaactaa tcttctagtc acaaatcaat ggggggagtt taaatgaata aagttgataa
    27541 agaaaagata caacatgcag taagagaaat actagaggca attggggaag acccagatag
    27601 agaaggtcta atcgagactc caaatagagt tgctaggatg tatgaagaga tattctctgg
    27661 tcttagtgaa gaaccaagag accatttaaa agttcttttt gcagatgaaa aacatgaaga
    27721 attagtattg gtaaaagata ttccatttta ttcatgttgt gagcatcatc tagttccttt
    27781 ctttggaaaa gctcatattg cttatttacc aaaaggagga agacttacag ggctttctaa
    27841 acttgctaga gtaatagata cattggcaaa aagaccacaa ttacaagaac gaatcactaa
    27901 aaatgctgca gatataataa tggaagaatt acaaccttat ggtgtcttag tagtagtaga
    27961 agctgaacat atgtgtatga caatgagagg tgtaaaaaaa ccaggttcaa aaactgttac
    28021 atcagcagtg agaggaatat ttgaaaagga cattgcgtct agagccgagg ctatgagttt
    28081 gattactatg aagtagcaat ttattataga tataatcatt tattgagata taaaatttaa
    28141 tttaagggag tgtcattaat tgtgtttgat tatggtaaaa gaacttatat aatgggaata
    28201 ttaaatgtaa ctccagatag tttttctgat ggaggagatt ttaataactt ggacatagca
    28261 atacaacatg caaaagatat ggttgaccaa ggtgctgata taatagacct aggaggagaa
    28321 tctacacgtc caggtcatag ctatgtagac tcagaagaag aactaagaag agtaatccct
    28381 gtaataaaaa aacttaaaca agaattggat ataccaatat caatagatac ttataaggca
    28441 gatgtcgcag aagaagcctt aaaattaggt gtcactatgg taaatgatgt ttggggtctt
    28501 agaagagata aaaacatggc ttctgtaata ggaaaatatg atgcagaagt gtgtataatg
    28561 cataatcaag atgggacaaa ctatgacaaa gatataatag cgtcaataaa agaatttctt
    28621 aaggaaagta tagaaatggc aataagttgt ggagtaaaaa aagaaaaaat agtacttgac
    28681 ccaggaatag gatttggaaa ggattttgaa caaaatattg aagtattgag aagattaaat
    28741 gaattaaaag acttagggta tcctatttta cttgggacat ctagaaaatc agtaatagga
    28801 aaagtacttc cagttgagcc taaaaaaaga ttagaaggta caatagctac tactgttctt
    28861 ggtataagag atggcgtaga tatagttaga gtgcatgatg tttatgaaaa tttaatggca
    28921 gctagaatga ctgatgcaat atatagaaag taggtataat ataatggata aaatattact
    28981 tagtaattta ggattctatg gatatcatgg tgttcttaaa gaagaaaatt ttctaggtca
    29041 gaaattcttt gtggacatgg aactttatat tgattcaaga gaagcaggtc ttagcgatga
    29101 tataaataaa tctgtaagtt atgctgaagt ttataatgtt gttaaagata ttactgaaaa
    29161 taaacaattt aatcttttgg aggctctagc agaaaatatt gctgaagaag tcttaaataa
    29221 atttatactt ataaatggag ttatggtcag agttagaaag cctgaggctc ctgtaaatgg
    29281 tatttatgac tattttggcg ttgagataag gagaacaaga gatgaataag gtttatttgg
    29341 gaataggtac taacatgggt gatagatttg ataatttatc aagagcatgt gaacttttga
    29401 aaaatagtga ttctatatat aaagtaaaag aatcaagttt atacgaaaca aaaccatggg
    29461 gatatacaga acaagcagat tttctaaata tgtgtgtaga aatagaaaca gagtttgaac
    29521 cttatgagtt gttagaatat tgtcaagaaa tagagagaga actacacaga gagagaatag
    29581 ttcattgggg accaagaact atagatgtag acatactatt ttttaatgat gtagtttcta
    29641 ctgatgaaaa actaacgata cctcatccaa ggattcaaga cagagctttc gttttgatac
    29701 cacttatgga tttaaatgaa gaacttataa taaatgaaaa aacaataaaa gaacatttaa
    29761 acctcctatc tgctgaagaa agagaagaag taaaggagct tgtaggttat gaaagaaaac
    29821 ctatttaata ataattcaaa gatagagata aatacaaata acaaaaagat aactgtaaaa
    29881 aaggataaac ttataatagc tggaccatgt gcaatagaga gttatgagca acttttggag
    29941 actgctaaat ttgtaaaaag tcagggagca aatattttaa gaggtggagc gtacaagcca
    30001 agaacatcgc caaattcatt tcaaggatta aaaaaagaag gtcttgaaat attaaaagcg
    30061 gttaaagacg aagttggtat ggctgttatt acagaactta tggatgtaag agatatggac
    30121 gaactgtata gtataagtga tataattcaa attgggtcaa gaaatatgca aaactttact
    30181 cttttaagtg aagtgggaaa acaagataaa cctattatgt taaaaagagg aatagcatct
    30241 acaattactg aatggatagg tgcttctgaa tatatagcaa tagaaggaaa tagtaatatt
    30301 ataatgtgcg agagaggtat aagaacgtac aatgattata ctagaaatac attagattta
    30361 gctgcagtac ccattattca aaaggaaaca ggtcttcctg ttgtggtaga cccaagtcat
    30421 gccactggag ttagatattt ggtaaaacct atgtcactgg catcttttgc atgtggagca
    30481 gatggaataa tggtagaagt acatcctgac ccagaaaatg ctttgtcaga tggtgctcaa
    30541 tccctttgct ttaatgaatt tgaagattta atgaaatcaa ttaataatta ttaaaagagg
    30601 caagagcctc ttttttattc gtaatcgttg ctaaggtaaa tttataagtt ataatttgat
    30661 agttagatta tttaatataa ataaagattt aatattgaca gaattaatat aaaaaataaa
    30721 aattttttga ttaataagaa ggatatcaat aaaaaaagtc gaattgtgta tattgagtga
    30781 ttgacaaatt tagaatatag tgtagaatag aaatgttaac acaaaaaatt acaccagaag
    30841 gtgataatat gaatgatata aaagacatta ttgaagaaat aaaagctaga tgcgatatag
    30901 caagtataat atctgaatat atgaatataa aacaatctgg agctaattac aaaggattat
    30961 gtccatttca tggcgaaaag acaccttctt tttatataaa tacatcaaaa cagatttata
    31021 agtgttttgg ttgtggtgaa ggtggagacg taataaattt tgtcatgaaa atggaaaatt
    31081 tggattttat ggatgctgta aaaattttag caaataaatg cggtatagaa attaacacta
    31141 acatgaatga agaaactagg attaaaatag aaaaatctaa gaaatttcaa gatattcata
    31201 cggaagcagc tagattttat ctttcaaact tattgggaag caaaaatctt gggtatgaat
    31261 atttaagaat aagaggacta gatgataaaa ttattaagaa atttggctta ggattttctt
    31321 tggattcatg gaattcactt atgaatactc taatcagtaa aggatataaa aaacaagact
    31381 tattggagtg cggtctaatt gcaaaaaata gagatggaac taattgctat gataaattta
    31441 gaaatagagt tatgtttcct atattcgatt atagaggaaa tataatagga tttgggggaa
    31501 gagtattaga cgattctttg ccaaaatact taaattctcc agacacatta atttttaata
    31561 aaaaacagaa cttatacgga ttaaattttg ctagaaaaaa tttagaaagt aaaactatag
    31621 tcttagttga aggatatatg gatttaattt cgctttatca gtatggtata aaaaatgtag
    31681 tagcaaccct tggaacagcc ttgactgaac aacaaggtat tttaataaag agatatgcgg
    31741 acactgctat tatatcttat gattctgatg aagcaggaat aaaagcgact ttaagagcta
    31801 tagatatact tactaaatta ggtatcaatg taaaagtgtt ggacctaaaa gatgctaaag
    31861 acccagatga atttgtaaga aaatacggac ttagtgatta taaaaaagct atggatgttt
    31921 ctactcacta cataaaatat aaaatagatc atcttaaaaa agaatttaac attcaaaaag
    31981 atgaagaacg agtgaagttc gcaaaagaag cttctaaaat tataaaacaa ttgacaagtc
    32041 ctgttgaaat tgatttttat acaaagtatt taagcaatca aatagatatt aatgtcgagt
    32101 ctataaagag agaagtctat ggtaaaaatt acaataaacc atataataac aaaaatcaaa
    32161 agaaaataga agaaaaagta attgaaaaag tcgaagtcag acaagacgga aaacaattag
    32221 ttgaagaaac tctaataaaa ataatgttag aagacaaaaa aattagagaa atagcgttac
    32281 ttaaagtaga agaaagtgat tttttattga aagaaagtaa agaaatttta aattacatga
    32341 ttaaaaatca agaattggac aaaataacta ttgacaaatt gaaaagttta aacatatccg
    32401 aagaatactt aaaagaatta aattcaattt ctttgaatag tataaactta gagaacacaa
    32461 aagaaataga aggaataata acaaatatca gaaaaaactc attagaagag caaattaata
    32521 gcttgttgag agaacaacaa gaattagaaa ataataatga tatgaaagag gtagatggta
    32581 gagttatgga aattgcttta aaaatagttg agataaataa aattttaaag agcttgtaga
    32641 gaaaggaggt ttgagtgtgg aaaataaatc gaataaaaaa gagttaaaaa aggttactgc
    32701 taagacatta atagaaaaag gaaaaaagca aggttctctg acacttgcag agataatgga
    32761 agctttttcg gagactgaac ttgataagga tcaagtagaa aatctttatg agactttagg
    32821 aaatttggga atagaaataa cagaaacaaa aaactataaa gctgatatag atttttcggt
    32881 tgctgatgac gatttaagta taggccactt agatgaagat gcagaggcaa tttcacatga
    32941 tgactcttct gcaatagaaa tagaaactgt ggatttatct ttaccaaaag ggataagtat
    33001 agatgaccct gtaagaatgt acttaaaaga aataggaaaa attcctctac ttaaaccaca
    33061 tgaagaagta gaatttgcta gaaggatgca cgaaggtgat gagatagcaa aacaaaggtt
    33121 agttgaagca aacttaagat tagttgtaag tatagcaaaa agatatgtag gaagaggtat
    33181 gcttttcttg gatttaatac aagagggaaa tttaggtctt ataaaagcag tcgaaaaatt
    33241 tgactataca aaaggatata agtttagtac ttatgcaaca tggtggataa gacaagctat
    33301 aacacgtgct attgcagacc aagctagaac tataagaata ccagttcata tggtagagac
    33361 tataaataag ctaataagag tatcaagaca actacttcaa gaacttggaa gagatccaaa
    33421 accagaagaa attgcaaaag aaatggaaat gacagaagat aaagtaagag aaataatgaa
    33481 aatagctcaa gaccctgtgt ctttagaaac accaatagga gaagaagaag atagccattt
    33541 aggagatttt attccagatg atgatgctcc agccccagca gaggcagcag catattcttt
    33601 attaaaagaa caaatagaag atgtacttgg ttcgttaaat gatagagagc aaaaagtatt
    33661 aaagcttaga tttggtcttg aggatggtag agccagaact cttgaggaag ttggaaaaga
    33721 gtttgatgta actagagaga gaataagaca aatagaagca aaagcactaa gaaaactaag
    33781 acatccaagt agatcaaaaa aacttagaga ttatttagac taaaaagata catcattaat
    33841 gtttttgtga taaatgccat gagataagtg atatctcatg gctattttgt agtaaagatt
    33901 taattttatt tttgtagtta tttcaatcta atatctataa cttggattaa atcctataaa
    33961 taaagggaat tgaaaggaga attatatttg aaattaacag atagattatt gaaaatagca
    34021 tcattagtgt cagatggtaa aaaaatagct gatataggaa cggaccacgg atacatacca
    34081 gtatatttgt taaaagaggg aaaagtacca tttgcagtgc tagcggatgt taataaagga
    34141 cctcttgaca atgcacataa agaagtcata cagaacaatc ttttagataa agttgattta
    34201 cgattgggtt ctggtataga gatacttgaa ataggggaag tagaagaagt gataattgcc
    34261 ggtatgggtg gaatcttgat aagtgagctt ctcgaggcta aaaaagaggt agcacataat
    34321 gtagaaaaat taatattaca gccaatgcaa gcacaagaag aacttagata ttacctttta
    34381 aacaacggtt atgaaatttt agaagaagtt ttagtgagag aagattttag aatatatgag
    34441 ataattgttg ccaaatatac aggtaaaaat actataatag aagatgagat atattatgag
    34501 gttggaataa agcttttaga aaacaaagat tctcttttta atgattttat agaaaaaaag
    34561 ataaagacat atagttctat agttaataaa ttagaaggaa aaaatgggga agctatagat
    34621 aagaagagaa aggaaagcga agttgtaata aaaaaacttg aaaatttaat aaaataaata
    34681 ggaggtatat atgctattaa agagtctaac tagaaaaatt gaaaaaaagt atcctctaaa
    34741 cttagctgag gattgggata atgtaggtct tatagttgga gattttgata tggatgtaaa
    34801 aaaggtattg gtatctttag aagctaatga agatgttatt gatgaagcta tatctaaaaa
    34861 tatagactta attgtgacac atcatccctt tatatttggg aaaattaata aaattaatag
    34921 tggagattta aaaggcagac ttatccaaaa acttattaaa aatgatatat cactttattc
    34981 tatgcatacg aattttgata tagcatttga tggtctaaat gactatttta tggaaattat
    35041 ggaatttggt aattcaaagg ttttagatat aactaaaagc gaaaatttat ataaattagc
    35101 agtatacgtt ccacataact attcagatga acttagaaag gttttaagta attcaggagc
    35161 aggtcatata ggaaattata gtgattgtac gttttcaata gaaggtgaag gacaatttaa
    35221 gccattagaa ggctctaatc cgtttcttgg aagtgtaaat gatatagaaa ctgtaaatga
    35281 agttaaaatt gaaactgtag taccacagaa gcttttaggt ggggtaataa gttctatgtt
    35341 agatgcacat ccttatgaag aagttgcata tgatttgtac aagttagaaa ataaaggtga
    35401 gatatttgga ctaggtagaa taagtaaact tgataaaagt atgacattag aaagtctttc
    35461 aaagaaaata aaagagaagt taaatatgaa acacatcaga gtagttggaa accttagtac
    35521 aaacataacg aaagtagccg ttgtaacagg agctggttca gaatttgtaa aaaaagctaa
    35581 gagacaaggt gcagaagtac taattacagg tgatgtaaag tatcatgagg cacaagatgc
    35641 acttgatatt ggtatgtgta tagtggattg tggtcatttt gatacagaag atatatttaa
    35701 aaatgttatg aagagatttt tagatgaatt tagtgaaatt gaagtaataa aaagtaatgt
    35761 atatttgaat ccatttagta ctatttagta gtgtttgatt ataaaaatac tatataaaaa
    35821 ataaatttga gtttaattag ggggataagg atgaaagtag cagtaatttt tcatagtgtt
    35881 tgtgggagta catatttatt ggctagagaa tataaagaag tgcttgaaga aatgaatatc
    35941 gaggtagata tctttagagt aagtgatgaa gttgcaaaaa cacttcctca atattatttg
    36001 ataaattcta aagaatacaa agatgaattt gaaagtatag atgtaattaa atctggaaaa
    36061 gaactattag attatgatgc tatatttatg ggctcaccaa catattatgg aaatgtatct
    36121 ggaccaatga agatgtttat ggatagtttt tctgatattt gggtaggtgc tcctttgagt
    36181 ggtaaaatat ttggatgttt tgctacagca ggttcacaac atggaggagg agaactagct
    36241 ttgcaagcta tgaatatatt tgcccaacat atgggaatga ctctcttgtc agttccatgt
    36301 tcagtaagag gaggtcatcc agcgtatggg atacttcata tagcaggaga taattctgat
    36361 ataagaccaa gtgatgatgt caagctaggt ataagagatt acctaaaaag gcttaatata
    36421 taagaatatc aaataaaaat acctaaaagt ggctgtatat acttttaggt atttttattt
    36481 gattataagc gtgaagcttt accagaaata tggtcaattt ctattttaac aacagttgtt
    36541 aagttcatat ccttctctat gtacttcatg ccttcttcaa agaatccttt agaatatttt
    36601 tttattattt ctacaagagc attcttcttt tcttcatttt ctacagtgca agcttttcca
    36661 aaaactatag cactagaata agtagtacta aatttatctg gaataacact ttcattagca
    36721 actactaaaa aagaaacttt gttgtttttc gatatattat ctaacttatg accatttctt
    36781 gcacaatgaa agtaaataga atcattaaag tatacataat ttacagcaac tccataagga
    36841 taaccatttt cagatatagt tgaaaaagta ccaaattcac cattttttaa tacttcaaca
    36901 gtatcttctt ttgtcatttc tcttttcttc aatctcattt ctttaaacat aatatcatcc
    36961 tttctaattt tgatgcttaa attaaatttt agaacattta taaagattat gctatgcttt
    37021 ttacaaattt taagaaaaaa atatagttta agataaaagg tagtgaaaga gaagttattt
    37081 ggagatttat ttatgatatt attgttaaag tagtacaaac aatactaata aatttaatag
    37141 ttggtttctt gttatttaag gtagtaagca tgtctataag aaggggaagt tatcgagggt
    37201 agaagttgtt atttataacg atattataaa aaaaatatta ttatataaaa taataaatag
    37261 taaagaggta tcaagtttaa tataatactt aatacctctt caaaattaaa ttataaaagt
    37321 gttatgggaa ttgtcctata catggtaagg agataataaa gaatgtatct ttaaagtctc
    37381 aactaaacag caaaatttat ccaataaaca tacaataaat gcttctttgt atctaggtgg
    37441 cttaacagta agtggccaca tatgttttaa aattatgtcc gtttcttttt catttatttg
    37501 aaaaaataaa gttgcatttt ttaatgcttc tctagggtga gtaaatccat gtaatccttt
    37561 tctatcacct ttgtaatgcc aatcataaag aaaaaagtcg tgtagtaaag ctcctctaat
    37621 aacactttga atatcaacac ttaagtgcaa ttttttacat aatagataac tatagtatgc
    37681 tactgacaaa ctatgttcaa gacatgaaac attgccatga tgaggaaatg ttctcataag
    37741 ttgaactttt tcagaagaaa gtatctcaga agcacattca ttaaaatatt tgatttcagc
    37801 ttttgacaac attatgaaaa tctccattct tagtaatata taaatactat acttcaacga
    37861 taatatgaaa tatttcttat cctacaaaat ataataccca ataatacaag taagatacct
    37921 tttatggtat tttaaagtaa gagagatttt attaggctaa acaatgaaaa aattaaaagt
    37981 aaagctatag caatatatag tatttttcca agaggattgc caaaatttat agtccatcca
    38041 ataccaaacc ttttattaac catcaatgat gggtcatttg gattattata catgatgcct
    38101 gctatccaat acttctcatc ttcttctgga gaataagatg agttgaattt taaatttggg
    38161 gaattaatat atgttgctat taaataaatt ataactaaaa gcgttgtaat attcatagca
    38221 gttgatagtt ttgtttttat tgataaaaga gtagtaaaaa actgtgttgt cattataatc
    38281 atcaatatta aaaacaaata accaatttta tttaaatatt taatgttttc aattctactt
    38341 tcttcaattc tatttgtatc tatcttaatc cttgacttta tggagccaat agaagttata
    38401 tacaataaaa ccattaaaca aaagtcagta ccgattaatt taaatacatt aagaaatgat
    38461 ttatctataa aaacatcagg agacccattt atgttccaat gagtggcaat tgagtctggt
    38521 aatttattat aattaaatgc tataaaaata ctcattccaa aagtaaatag tattggtatt
    38581 aaatagatta ttttaaattt ttttattatt ttatttttct tatttataaa atccatatct
    38641 actattaatt ttgaatctat atctatatga cctaatttag aataaatttc tgatttaagc
    38701 ttcttagctt ttttatgtgt atgaatatat agtatacttt catataataa aaaacctata
    38761 ataccaataa aataggaaag ttccaattta ttaaatataa aaaccagaat aaaaactaat
    38821 ataaagtcta taataaaacc taaagataac agttttttaa attctttatc taactttata
    38881 aagtaattat aatttttatg ctcttggtct atagaaactc catagatgtg ttttttacct
    38941 acaaaatagg gcataaaata aaatgtagca tacatcaaaa ttagaactgg aaatattgta
    39001 aatagacatt caatcttact cataatattt cacctcatac ttatcaaata aacttgaact
    39061 taatgacaaa aattcttctt tactaatacc tgataaataa gattttgcca ccaataattc
    39121 caagtcaagt ttattttttt gacgagttgt attatctatt ttcattggta gttgagcaac
    39181 aatagcacct tttcttctat caatatttaa gaatccctct tcttttaatt cattgtagga
    39241 tttgttcact gtatgtaaat ttacaccaat attttcagcc atacttctta cagatggaag
    39301 tgattcattt attttcaatt ctcctgaagc tatagcctta gttatttctt ctttaatttg
    39361 tacataaata gatttatcac tattaaaatc taaatttaaa accataaaaa acctccatta
    39421 tgctttataa atgaaaacga ttgttatata catagtataa cataaaaaat aataacaaac
    39481 acaaaatata aatttatatt ttgtaaaaat tgtaatttaa aaaataacat aatttttatt
    39541 aaaataatac agatactatt tatagataga aataaaaatt agaaaggaga aaaaatatgt
    39601 taacatattt aatgaaaaga caaatgcata aaaaagatga tggaatgagt aaaccaatga
    39661 tgattacagg agctgtaatt acaggtgtag gagcatatgc tgtatacaaa atggtaaaaa
    39721 gccgtaaatc taattctcaa atggtaaata caaattatta tggaaatagc gattatgatg
    39781 attataaata tgacgaattt gattatgact gtgattgtag agacacaaaa caagattatg
    39841 aatatgaaga attaaaaaaa aaagtaaaag aatttaattc aagaagaata aattgtgaaa
    39901 attgtccaca tgtttctcaa gaagaaatga tgcagtatgt aaatagtgtt aaagatgtaa
    39961 aagatgagat tgatttacat gaagatgcta aaaaagataa tatttacaaa gaagatgaat
    40021 acaaaaaata tgaagaagaa taagtaatat tctgtataac tgaacaaata gtaatgttaa
    40081 attatttaat atctagtttt aatatgtaag ttgtaactta acttgaaaga atcagtatca
    40141 ttctttagat agttaagtta caacttacat tttgttttta taaattttaa tagttctttg
    40201 tgactatttt gtaattttag aaatatttta gtatccaggg taattgtgta taataaaact
    40261 tgtataggta gatagtgtaa aattaagcag atagtttaaa attaaaatga aaagaaagga
    40321 aatagttatg ttaaagttgt tgatagttga agatgatagt actatctctt ttggaataaa
    40381 gtatgcttta gaacaagaag gatttagtat agatatatca aaggatttat cgagtggaaa
    40441 agaaatgatt tcttctaatg aatatagtat gatactgttg gatgttacat tgccagatgg
    40501 aactggttat gagctttgtc aatatataag aggattctct caggttccta taatattctt
    40561 gactgcttgt gatgaagaag taaatattgt aatgggtctc gatatagggg gagatgatta
    40621 tataacaaaa ccttttagaa ttagagaatt aatatccaga ataaaagccg ttttgagaag
    40681 aaaggttaat accgtagaag aaagtaagaa aatacttaaa tttggagagt taagtatata
    40741 tacattagaa gctagagtat ataaaaacga taaagaaata tttctgacat cagttgaata
    40801 taaacttctt ttaattctta tacagaataa aaatactgtt ttaagtagaa ctcaaatatt
    40861 ggaaaaactt tgggatgtaa cttatgattt tgtaaatgac aatactctaa cagtttatat
    40921 aaaaaggctt agagaaaaaa taggtgatga tttatgtgaa ccaatctaca tagaaactgt
    40981 aagggggatt ggctataaat ggataggaag tgaaaatagt gtatctatat aatccagaag
    41041 tgaagaagtt tttaagtaag tatgctactt taatgtttat tgttattata atatctattg
    41101 gatttagtgt aatcaacgtg tctttaacta aagagatggt tgtaagaaat aatcaagcta
    41161 taatagggac tttatcgagt aagtatccaa acttagaaag tgatatagta gatataataa
    41221 ctcaaggcaa gtctacggaa aacatagatt atggtcagaa aatattaagt aagtataatt
    41281 atgataaatc tattaagata aactctgagc ctattatttc taaattagtc ttagagacta
    41341 tcaaaataaa tataatatta gtatgtataa tatttatttt gatacttgtg ttggttgtta
    41401 gatattttaa gtcaatttac aatgatttat cagatatgac taagtatgtt tactatagtt
    41461 cagagggtaa gccatttgat atgaaaaaca agaatcaaga aggacagata ggtcttctaa
    41521 aaactgagct cttaaagatg acaacaattc ttaatgaaaa ggttgaactt ttaaaaagag
    41581 aaaagatatt tttaaacaat acaatttccg atatatcaca tcaattaaaa actcctatga
    41641 cttctttaat aatgttgaat gatttattat ataatgatgt accatatgaa gtaaaaatcg
    41701 attttttaaa taagataaaa aatcaattaa atagaatgga ttggctgatt aaaagcatgc
    41761 ttaaattatc aaaggtagaa gcaaaagtga taaattttaa aaaggataag gtgaaattta
    41821 gtgaactcat acatagagca atgcaatcta tgaaaatacc tatggagatt aaaaatcaaa
    41881 atttgactat agagggaaat gataatgtat cttatattgg tgatattgat tggtctgtgg
    41941 aagcactagt gaatataatc aaaaattgtg tagaacatac tcctgaattt ggaaatataa
    42001 ttataaccta taaagaaaat ccattatttt ctgaactaat aataaaagat gagggtgaag
    42061 gtatagataa aaaagatata ccacatgtat ttaaacgatt ttatagaggc agaagtagtt
    42121 caaaagaaga tagtgtagga ataggtcttg caatgtcaaa atctataata gaaagccaaa
    42181 gtggcgatat atatgtaaac agcgaaaaag gtaagggtac agaatttcat ataatatttc
    42241 ataaaatgta tgatagtgac taatctgtaa tatttaattc actttatagt aatagccaaa
    42301 gcctaatata tgaagtatag aatataaata ttggaggtag ttatggaaat tttaagagta
    42361 gaaaatctaa ctaaaagtta tggcaaaaat gaaacaaaag ttgatgctat aaaaaatgta
    42421 agtttatcag ttgaaaaagg aacatttata gcaataacag gtccaagcgg aagtggtaaa
    42481 agtaccttat tacatttatt aggaggagtt gacaggccaa ctagtggtaa ggtttatata
    42541 aatgatgttg atatatataa tttaaaaact aaggatttgg ctatatttag aagacgaaat
    42601 ataggtctta tatatcaatt ttacaactta attccagtat taaatgtgaa ggaaaacata
    42661 ttgcttccag cagagcttga caatagagat attgatggag aatattttga tgatttaatg
    42721 gagacattag gtctaattga tagagaaaag caccttccaa accaattgtc aggaggtcaa
    42781 caacaaagaa cctctatagg acgtgcatta attaatagac cttctataat attagcagat
    42841 gagccaacag gtaatttaga tagtaaaaat tctaaagaaa tactagaatt gttaaaatta
    42901 tcagtaaaaa aatacaacca gacactaata atgataactc atgacaaaaa tatggcactt
    42961 caagcggata gaattataag tatagaagat ggaatcataa aaagtgatga ggtgatgtag
    43021 atgaatttat atacatcact aactattaga tacttaaaac aaaataaaag aagaactatt
    43081 gtaaccataa tcggtataat actttcgaca gctttaattt gtggtatagg aaatatattt
    43141 gaaagtttta tggattatca aattagagaa actataaaga atgatggaag tttccatgtt
    43201 acattttatg atgtaaatag aaaaaatgtg gaatatgtaa cgaaatcagc tgaagtagaa
    43261 aaacatgcat ttaccaaaca gcttggatat tctaaattag aaaatagtga aaatggcata
    43321 gtaagtataa agcaatatga taaaaatgca ataaacggat ataaaatttc tataaaagaa
    43381 ggtagatttc cagctaagga aggagaaatt gttctaagtg acaatattat taatcttata
    43441 gatgataact taaaaatagg ggataagata actttaaaag taggagatat gtttgattca
    43501 agtaagaaga agattgatag tatggtattt catgaggggg attatatagc aaatgaaaag
    43561 gatagaactt ttgaagttgt tggtataatt aaaaaacctg gatttgaacg ttacaatgga
    43621 attgcaactg ctataactta ttttaatcct atggataatt acaatgatac aattaatgta
    43681 tcagtagcac taagaaatcc taaagatgtg tatgaaataa gtaataaaat aagtaaaaat
    43741 atatattcaa ataaagataa agaagcagtt tctgatatga taaaatacaa tgaacattta
    43801 ttgaggctac aaggtgctag taagtatgcg aatataaata gctctataaa gtctataatt
    43861 atagcagtaa ctattttagt aatcatatgt acaattgcta cagtgtacaa ttcatttagt
    43921 atttctataa ctgaacgaaa aaaacagttt gggatattaa attctatagg tgctacatct
    43981 agtcaaatta aaagattagt ttttatagaa ggtattataa ttagtttaat tggtatacca
    44041 atagggataa tatctggaac agtagccata gacttattat ttaaaattat aaataagcat
    44101 tttactgaat cggttgttac tcaaatgagt ttgaagatag tgtataatcc aataataatt
    44161 attgttagtg taataatagt tttgtttaca atatttatat caattctatt acctgcaata
    44221 tcggcttcta atatttctcc acttaatgta attaaaaata atggagagta taaagtggga
    44281 aaagttaaaa gttcaaggct tattaaaatg atttttaaaa ctgaaggagt acttgcatat
    44341 aaaaatataa ggagaaatag aaaaaaattc acagtaactc ttttttcttt aatggtaagt
    44401 ataataatat ttatatcttt tagcggattt acattacttt tattaaaagg tgaagaaata
    44461 agaaattctc aaagaaacta tgatttgtat ttaactgcaa agggtacagc taagtctata
    44521 gatgatacta taagtaaatt agaagctatt tctggaataa aaaactttga attagcaaca
    44581 ggacaatata tgtctataag agtaagtgaa aataaaataa ataaatctaa ggaagattta
    44641 atcagaaaat attatcaaaa atataagatt ggagatagtt atgaatatga tttcagtaat
    44701 aatgaattaa tttttccagg agattttgca gtaaaaaaca aaacaaataa cttaatacag
    44761 ggttctttta ataaggagag agcaattgaa gaaaatggtg ttatattagt tagaaaaagc
    44821 gcatttgaag aaaatgggaa gaaaggtgta gtagaactta ccaactataa agtaggagat
    44881 acagtaaatt gtgaatatct tgatgaaaat gctttaagta aaaaaataaa ggtcaagatt
    44941 cttgctataa ctgatgaaga acgtctaggt cttggttatc aaaatatggg gttacaattt
    45001 ataacttatg atgaagttgc taaaaattta aatttaaaat taagtaaaag ccttatattt
    45061 attgattcag gaggaagcat taagactaag aaggaaatag aagctttggc aaataaaaat
    45121 aattttaact tttatgatga aagtgcaagc aatgaaagtg aaaaacaaga tttaaaggtt
    45181 attaaaatat ttgtatatgg ttttatagta gtgatatctt tagtaagtgt tacaaatatt
    45241 ctaaatacag taagtacaag tataaatcta agaaaaagag aacttgcagt aatacaatct
    45301 ataggggtta ctcctaaagg atttaggaaa atgatatatt tagaaagttt tatatatggt
    45361 atactgtcat tgttgtttgg aataccaatt agtattggta taattctaat tatgaacaaa
    45421 cttatttcag gtgtaattga attttctccg attataccgt ggacagccat agttatctgt
    45481 atagtaagtg tttttataat aacttttgta gcagggtata taccaatgag taaattgaat
    45541 aaagaaaata taatagataa ta
//
LOCUS       DAEPXF010000010        44266 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain HB27
            SAMN13376389-rid12479423.denovo.011, whole genome shotgun sequence.
ACCESSION   DAEPXF010000010 DAEPXF010000000
VERSION     DAEPXF010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13376389
            Sequence Read Archive: SRR10518432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 44266)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 44266)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001069624.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001069624.1
            Genome Coverage       :: 315x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/15/2021 01:54:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,165
            CDSs (total)                      :: 4,076
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 89
            rRNAs                             :: 10, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 10 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 19 of 69
            Pseudo Genes (incomplete)         :: 44 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 10 of 69
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..44266
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13376389-rid12479423.denovo.011"
                     /strain="HB27"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="China: Hebei"
                     /collection_date="2017"
     gene            266..730
                     /locus_tag="KRL67_000505"
     CDS             266..730
                     /locus_tag="KRL67_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CtsR family transcriptional regulator"
                     /protein_id="HBH1521410.1"
                     /translation="MATMTDIIEKFIKDLMEEDNSIQIQRNELANLFSCAPSQINYVL
                     TTRFTIDRGYYIESKKGGGGYVQIEKIRKSKDGHIRELLNEKIGSQISYKKAKELLEG
                     LKESDLINERELKLILYAIDDKSLCMPIYELKEKVRSNILKNIIIGLFSIEE"
     gene            737..1231
                     /locus_tag="KRL67_000506"
     CDS             737..1231
                     /locus_tag="KRL67_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UvrB/UvrC motif-containing protein"
                     /protein_id="HBH1521411.1"
                     /translation="MLCQKCNKNKASVYYNKIVNGEKTEMYLCSECAKENTEMNFNLD
                     MPFSMMDIFSNLGFQPKKELEEKLVCPKCNTTYSEFKNNGRFGCSECYNAFSSQVNPM
                     LQNIHGHIEHTGKAPKKSFYKISVENEIKELKEDLDRAIKNEEYELAAQFRDKIKYLK
                     GSID"
     gene            1247..2272
                     /locus_tag="KRL67_000507"
     CDS             1247..2272
                     /locus_tag="KRL67_000507"
                     /EC_number="2.7.14.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein arginine kinase"
                     /protein_id="HBH1521412.1"
                     /translation="MKENIVMKSRVRLARNLNNYPFPNKLDKECAMEIIEKVKNAFIN
                     SNLEQKEEFDFYKIEDLDQSKKMLMVEEHIISPDLAENDKSAVIVKKDKTISIMINEE
                     DHIRIQTICDDLNLEYAYSVANEIDDLLESSLEYAFNTKLGYLTSCPTNTGTGMRASV
                     MMHLPALSQLGYMDELYKISSQIGIAIRGIYGERTEALGNIYQISNQLTLGRTESNII
                     ENVSGLTKDAISKEIKAREILQKKLGIKLEDKIFRSIGTLENSRVMSSAEAMSHLSNI
                     KMGIEMNYIDKLDLKAIEQLMIGIQPAHQSIMYKSDDVENRDINRATYIRETLEKLRG
                     GMNCELQ"
     gene            2259..4706
                     /locus_tag="KRL67_000508"
     CDS             2259..4706
                     /locus_tag="KRL67_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HBH1521413.1"
                     /translation="MNFNRFTQRAKKAIDLAFESAKSLGHNIVGSEHILLGLLREEEG
                     IAAKVLSKVGFTEAYLEGKIVDMEGKGEEISEDIVLSPRSKQILELSGMFANKLKTNY
                     IGTEHILLAIIQEGEGIANKILNYAGVNDRTLAQLTIDMMGISDKNQYKAENSYTGNQ
                     NQAESKVLDKYGRNLTLYAKQNKIDPVIGREKEIQRVIQILSRRTKNNPVLIGDPGVG
                     KTAIAEGLATNIALGNVPETLKNKTLYSLEMGSLLAGAKYRGEFEERIKEVVDEVVKN
                     GNIILFIDEMHTIIGAGSTGEGSIDASNILKPALARGEIQVIGATTIDEYRKHVEKDS
                     ALERRFQPVMVDEPTKEDSIKILEGLRDKYEAHHKVKITDDAIKAAVELSTRYISDRY
                     LPDKAIDLIDEAASKVRLKENTPPSEIKKLELEIENIDKEKEEAVRCQDFEKAAKIRD
                     EQGLLKKQLEDVRERWNKSSKHSDLVDGEVIAEVVGLWTGIPVNKILEEEADRLLRLE
                     EILHNRVIGQEQAVKSISKAIRRSRAGLKDPNRPIGSFLFLGPTGVGKTELSKALAEV
                     QFGDENQIIRIDMSEYMEKHAVSRMIGSPPGYVGHDEGGQLTEKVRRNPYSVILFDEI
                     EKAHPDVFNILLQILDDGRLTDSKGRTVDFKNTIVIMTSNVGASTIGRQKTLGFSIAK
                     GDEEEKSQYEKMKENIMGELKQRFRPEFLNRIDDIIVFHSLNENHISKIVLLMAAKLQ
                     ERLKEMDIKLEMSDEAVKLISKSGFDLEYGARPLKRALQKELEDELSEAILKGNVKKG
                     SNVVAKVKDEKIVFETK"
     gene            4886..6259
                     /gene="radA"
                     /locus_tag="KRL67_000509"
     CDS             4886..6259
                     /gene="radA"
                     /locus_tag="KRL67_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HBH1521414.1"
                     /translation="MAKIKTKYVCQSCGYETAKWLGKCPECTKWNTFVEEIDQKSTKK
                     EVFIIDKSSSKPVSINSIESKEEERFTTDINELDRVLGGGIVKGSLVLVGGDPGIGKS
                     TLLIQVSSNVANLGKTVLYITGEESESQIKMRAKRLGINSENLYIFAENNLSIIESYL
                     ESVNPELIILDSIQTVFSPEISSAPGTVSQIKEGTSKFMKISKKMGISTFIVGHVTKE
                     GSLAGPKLLEHMVDTVLYFEGERYNTYRLVRAVKNRFGSTNELGVFEMRDLGLVELDN
                     PSKILISEKPKDVAGSVIISTVEGTRPMLLELQALVSPTSFGIPKRTSTGVDYNRVGM
                     LLAVLEKRVGLQIQNQDVYINIVGGIKINEPSIDLGIAISVASSFRNIPIDEDIAVTG
                     EVGLTGEVRAVSFIEKRIAECKKLGFKKIVVPRSNYDVVKETKGIEMWPVDNLRQAIN
                     IVLGRNQ"
     gene            6262..7332
                     /gene="disA"
                     /locus_tag="KRL67_000510"
     CDS             6262..7332
                     /gene="disA"
                     /locus_tag="KRL67_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA integrity scanning protein DisA"
                     /protein_id="HBH1521415.1"
                     /translation="MENFLDNKNMLYALKMISPGTPLRLGLNNVLRAKTGGLIVIATN
                     EDVMKIVDGGFAINAEYSPSYLYELAKMDGAIVLSGDVKKILFANAQLIPDYFIETSE
                     TGTRHRTAERVAKQTGAIVIGISQRRNVITVYRGNEKYVVEDISKIFTKANQAIQTLE
                     KYKTVLDQAITNLNALEFNDLVTIYDVALVMQKMEMVMRVTSIIEKYVIELGDEGTLV
                     SMQLEELMGTTRIDQKLIFKDYNKENTEIKELMKKVKNLNSEELIELVNMAKLLGYSG
                     FSESMDMPIKTRGYRILSKIHRLPTAIIENLVNYFENFQQILDASIEELDEVEGIGEI
                     RATYIKNGLIKMKQLVLLDRHI"
     gene            7421..8515
                     /locus_tag="KRL67_000511"
     CDS             7421..8515
                     /locus_tag="KRL67_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN/TRAM domain-containing protein"
                     /protein_id="HBH1521416.1"
                     /translation="MIRKVTRILFILLGFTIGITTYLTLMKDFEILTFGKETYGYIAA
                     VVAGIIIAILGYLIEPWVVNKVKEIAKIVDKELSKYPQTDILLGSMGLIVGFVIAYLL
                     SGLVNRIPIVGGILSLLLYLFLGYLGMKVALKSKNDLFNIGKLGRLANPIKDKDKDKE
                     NKKEVKAIPPKVLDTSVIIDGRIADICKTGFIEGKLIIPAFVLEELRHIADSSDDLKR
                     VRGRRGLDILNIIQKELNIEVEISERDFDDIAEVDSKLLKLAQVLNGKVVTNDYNLNK
                     VAQFQGVEVLNINELANAIKPVAIPGEDMVVQVVKEGKEAQQGVAYLDDGTMIVVDGG
                     RKYMNETIKVLVTSVLQTPAGRMIFAKPKN"
     gene            8644..10821
                     /locus_tag="KRL67_000512"
     CDS             8644..10821
                     /locus_tag="KRL67_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH1521417.1"
                     /translation="MRKKIISFLATFIIILTSASQYSFADDISTRGKVIFIDMNRTSM
                     SNMLRIKSLREELDNRGYIGLMNIRGDKGSDDRRSYASMGAGGRANVANEEDINFESS
                     SKDRDIVFESATGKSAKGINNLTINKSINENLNFGEYGSVLGSLGQSLSDNGLKASVL
                     GNSDIIENGQLIKNRNLCLTAMDEYGRIPNGNVDTINKKDLSMPYGISTDYDKLIVET
                     KEAYKNNDVVFVELGDTYRLDLYKPNLNEKAYESMKDNIEKNIDVYLKNIFSIVGEND
                     TVYIASAFPSDLDYKNKRRLSPIIKLNGEGKGILSSSTTRREGIVTNLDVGVEILDNF
                     NIKNQNMVGRKYELINRDDNKEFLMDEYQKIVSISSIRSTILNGFVSIVFLSWIVAMI
                     AILFRKHISKNYKETTFFILKELLKIGIVMPLSFMITPIMNFKTPLAISLGIIIITLT
                     IYLISKVLIKNDLKNMLFFTGLTIVIMVIDAGFGSYLMKSNVMSYDCIIGARYYGVGN
                     EYQGVAIGSAIFTFAILLTYKNIPKWSVIVFSLLILITSASPIMGANVGSAISECVAF
                     LLFILLIYNVKIDFKKIVLLGIAVLFVLGAFVAIDMILGSNSHLGMFVKEIYFNGPGE
                     IIQTFSRKIEMNLKLAQTSAWVNILLTGIGVILALMINQIRYFRQLMDEYPIVFKGFI
                     ASIAGCLVTLLVNDSGIVSSATAFIYVIVPMITLSINLIALKE"
     gene            complement(11007..11852)
                     /locus_tag="KRL67_000513"
     CDS             complement(11007..11852)
                     /locus_tag="KRL67_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBH1521418.1"
                     /translation="MNRNNFSHEIVEPIDNLDVNFKLFDDSGSYVANHWHNSLEIIYI
                     TSGDLQINMEGYTYNLKANECMFINSGIIHSTRCTYQNTSILLQVPLSFLNKYIPDFK
                     NCYFDFKVNANDNNYKKNSSKVKLILENMREIKLSSPPAANLLFTSLLFELLFELYTN
                     FKISVGNKNMKKTVLDLSKFEPVLEYTKINYKMPISINKIAKVAHLQPEYFCRKFKQY
                     MGQTYLEYLNDVRISHIYKDLINTNDTLCSILEAHGFTNTKLFYRIFKEKFKCTPKHI
                     RKKST"
     gene            12068..13234
                     /locus_tag="KRL67_000514"
     CDS             12068..13234
                     /locus_tag="KRL67_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulase family glycosylhydrolase"
                     /protein_id="HBH1521419.1"
                     /translation="MYIKGVNLGGWLVLEKWMTSSLFEGTEAEDEYYLPRQLSREVYE
                     SRIKTHRSEYITERDFATIKSMGFNSVRIPVPYFIFGDCEPFIGCVKELDKAFVWADK
                     YGLSILIDLHTVPGSQNGFDNGGISGVCIWSQNPEYVGFTLNVLERLARRYGMRHELY
                     GIQILNEPITERMWNIMNVPNRFKAADKEMARGSKPNSLEFLRDFYIKAYKVMRPYMR
                     EENVIVFHDAFELKAWKDFMREEEFKNVVLDTHQYLMLAEADGCEQSIDSYLKYIREN
                     YAKDILQVQKYFPVICGEWSLFNSYACGIDTDGGQSPLNGIESNIDKLSKDEKRELYR
                     KIAKAQLDAWRNGSGHYYWNYKLLLDTVNEEGWIGWDSWDLGKCVAQEWYPIEY"
     gene            13256..14902
                     /locus_tag="KRL67_000515"
     CDS             13256..14902
                     /locus_tag="KRL67_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HBH1521420.1"
                     /translation="MIKNPILPGFNPDPCICRKGDDYYLVVSSFEWFPGIPVYHSKDL
                     KNWELYTHILTDETKIDLKKLPSSKGIWAPCLTYCEEEDLFYIVYGIMNSMNARYFDV
                     DNYLITSKDIKGEWSEPVYLHSSGFDASIFHDDDGKKWITSLDWETREGYEKPGVICL
                     VEYCTKKKEIVGYPKRIWSGGTDRGCIEAPHITKRGDYYYIMCAEGGTGYGHSVTMGR
                     AKNIWGPYEKDSMNPIVTSIPGDFYERHDPDHLKPKYYNPESKLQKSGHGSYIETTSG
                     EVYLVHLTSRPFVPELRCTLGRETAIQKMKWTKDNWLRMEDESNLAKEYVSESKLEEH
                     LVSSIPSFDDFNSNELGLQYYAPRISPLSFADVKSRPGYVRIRGQESRTSLNKVSILA
                     RKLTSVYARITTKMEFYPEVHQHSAGLIMYYDNMNYINLRKYYSETLGQSALSIIHLE
                     NGEKTEFLNTRTPIKDIPIYLRLYIQGRKSYFEWSYDEKNYQRIGKVFDTTKFSDEYC
                     KYGEFTGTFIGLTCADRVKHKHYADFDFFEYIADESKDVD"
     gene            14999..16372
                     /locus_tag="KRL67_000516"
     CDS             14999..16372
                     /locus_tag="KRL67_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBH1521421.1"
                     /translation="MNTEVNVKATTIRYEKISFFERISYGCGDLGCNIIYSAMSAFLL
                     FYYTNYAEVSAAAVGSIMLVSRILDGFSDLTMGIIVDRTKSKYGKARPWILRMAIPFA
                     IAAILLFSVPSNLGITSKLIYIFITYNLVSTVIYTAINVPYATLNSLITQDQYERGVL
                     SIFRMILATCGTLLINGLTLPLVEYFGNNLSAWTKTFFVFGIASIMVFFITFTGTKER
                     VKAVKQDRNEVIPFKIGIKSLFRNKYWIQITLCLVCIFIVFAINGGSSVYYAKFILGD
                     EKLFGPINMVSNISQIIAMFMVAPFIKKFGKRNVLIVGSIILISSNVMFIIVGQNYIG
                     VIFASVIKGIGSAGIASTMFAIVSDTIEYGEWKTGYRTEGLINSASSFGFKVGNGLGS
                     AILGAVLSIGGYVGTSATQSDLAIVSIKVCFIYLPIFITVLQIIIMSFYKLDKEYSTI
                     LNELNTK"
     gene            16820..17773
                     /locus_tag="KRL67_000517"
     CDS             16820..17773
                     /locus_tag="KRL67_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding transcriptional regulator"
                     /protein_id="HBH1521422.1"
                     /translation="MKKIGDLRLMMKCCSLYYEDNLNQQEIANQLGISRPTISRILKE
                     AFEQGIVKIQIVDVLKNDYQKIERSLERKYKLKEVIVVDDKQDALTQKQELARAVSEY
                     LTRVVKENDIIGVSIGTTLKEIPRYVEKSNCKNVTFIPLLGGIGDNEIDIHANQIAVS
                     LARAFGGDFKLLHAPAVMSDLSTKEKLCKDEKIKEVLDLIDKTTIAIVGIGNPMSLNS
                     TIMASGYMNEADIEDLKKYNSIGAICLQAFDKEGKTSILEFNQRVLGVKLEDLKKIKR
                     TIGVASGDEKIEAIKASLKAKFINSLAINHSLALKLLEDCD"
     gene            17811..18773
                     /locus_tag="KRL67_000518"
     CDS             17811..18773
                     /locus_tag="KRL67_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent dehydrogenase
                     E1 component subunit alpha"
                     /protein_id="HBH1521423.1"
                     /translation="MSISKETLLEMYKRMNQARKFEEKVSWFFARGMVHGTTHLSVGQ
                     EASSVAAVMALEKGDLVSLTHRGHSQFIGMGIDLNKMMAELMGKETGFCKGKGGSMHI
                     ADIESGNLGANGVVGGGLTIAPGAALTQQYKKTGKIVLCSFGDGASNEGTFHEGINLS
                     SIWKLPIIFYCENNLYGMSTSIKRHMNIENIATRAASYGIEGISIDGYNPIEVYETVQ
                     KAAEKCRRGEGPVLIESRTYRWLGHSKSDANVYRTKEEIESWKAKDPIEFLKNYLIEN
                     NLSNEDELDKIQEFAKQSIEDAVEFAQNSPNPKIESLLEDVYAD"
     gene            18798..19784
                     /locus_tag="KRL67_000519"
     CDS             18798..19784
                     /locus_tag="KRL67_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HBH1521424.1"
                     /translation="MSTRELTYAQAIKEAMSEEMRRDENVIFMGEDIGIYGGAFGVSV
                     GMIDEFGPERVRDTPISEAAIAGAAAGAAATGLRPIMEVMFMDFVTISMDAIVNQAAK
                     MRYMFGGKAQVPMVVRCPGGSGTGSAEQHSQSLEAWFCHVPGVKVVAPSTPADAKGLL
                     KAAIRDNNPVIFVENKLLYRKKGVVPEDDYVIEIGKADIKREGTDVTVITYGRMLQSV
                     EEAAETLSKENINVEIIDLRTLYPLDKETIVKSVCKTGRVLICHEAAKTGGLGGEISA
                     LITESESFDYLDAPVKRICGKDVPIPYNPDLEKAVVPRVDEIEEAIKSLIVR"
     gene            19797..20843
                     /locus_tag="KRL67_000520"
     CDS             19797..20843
                     /locus_tag="KRL67_000520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo acid dehydrogenase subunit E2"
                     /protein_id="HBH1521425.1"
                     /translation="MVANKIKATPAARSQARKDNIKLDRLIGSGENGRIHLVDVLNYL
                     KDNKANTTPLARRIAEDLNIDLETIVGTGYNGKIRKCDVEKLTAKETIVSTNTSKSNE
                     KKEMKIENENSSMFNTVEGIFEKPNPMRATVAKRMSESYFSAPVFTFNIEVDATELKA
                     LRAKLMDTVKESTGVKLTMTDLIVMAVSKILPNHQALNSAWTDEGIFRYKDVNIAIAV
                     GLDEGLYVPVVKNANKKSLKEIAKESKELAEKVKTGKLMPADQEGNTFTISNVGMYGI
                     TTFTPIINMPSSAILGVGATQDKFVPVNGEAKIKPIMNLSLTSDHRVIDGTVAAKFLK
                     DLKELLENPLSMLV"
     gene            21010..22740
                     /gene="lpdA"
                     /locus_tag="KRL67_000521"
     CDS             21010..22740
                     /gene="lpdA"
                     /locus_tag="KRL67_000521"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HBH1521426.1"
                     /translation="MSVEVIMPKAGVAMEEGTIVSWLKQEGEEVKIGEPILEITTDKV
                     NMEIESEGEGTLAVIIHKEEGEVLPVFTVIGVIAEKGENQEEVKAKYSSGNISKEDIV
                     EENQNTEVKEEKINKKECNHDYDVVVIGGGPGGYLSALKAALLGGRVALVEENILGGT
                     CLNRGCIPTKTYIKTAEILEEIDQLSKRGVKVTVDKEQDIKKAIKYKNRVVKKLTAGV
                     GGLLKSRDVEVFNLKASVKEEHKVILSDGKVLDTENIIIATGSKVRILPIKGIESNLI
                     ITSTEALDLETVPEELVIIGGGVIGCEFAEIFNSRGSKVTIVEMEDRVIPRMDKELSE
                     SLKYSFSKKGINVLTKKKVSEFKEEGNNILVCIEGEEPIKADLCLYAIGREANLSGIE
                     DLDIKIDKGSIVVNSKMETSIPSIYAVGDVTGGVMLAHAAFKMGEVAASNALGMNKEV
                     DLGALPSCVYTIPEVASVGITEEDARKKYNVKVGKFNFAGNGRALASGQEQGYVKVVA
                     DAKYGEILGIHMFGCGVAELINHAAAFKALEIPTDEASELIFGHPCTSEALMEALADV
                     NGECLHLPKK"
     gene            23158..26106
                     /locus_tag="KRL67_000522"
     CDS             23158..26106
                     /locus_tag="KRL67_000522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBH1521427.1"
                     /translation="MRQRIKNLIENEDKKNPLTDEAISSRLNIRREDVTFLRNELKIE
                     DSRQRRKVVLIKAIKDILKEEKTINKNDITRRLNSIGFTVSRFTVIQYLKEIQDSGDL
                     KLDKESDDKKEEIINNRENTDIAFDKLIGSSGSLSTIIKLAKAAILYPPHGLHTIIMG
                     PTGVGKSELAECMYKFAVESSRFPKNAPFIVFNAADYSENPQLLLSQLFGHVKGAFTG
                     ADENKEGLVSKADGGILFIDEIHRLPHEGQEILFQLIDKGMVRKLGETRLTHKIDVMI
                     ISATSEPIDSHLLNTFKRRIPVTIEIPELVARPLNERFDIINNFFLIEAQRMGANIHI
                     KSDVLKALMLYDCIGNVGQLRSDIQVACARGLLNQLTNKQKEVNISISDVPGYVKQGL
                     IKIRNCRGKIENYVDGDLIIDSNLQGEIEKKNEDIYTFPDEIYKLTERRHIDLLEQGL
                     DFDVINRIIGGELESCIQKYIKQVKKVSISNNLPDISPIVGKYIVELTYEVIKIASQY
                     LSSLEPNLHLCLAVHLKAAFERLKEGKVISNAHLKEIIGNYPLEYSVAEKVAQYLKER
                     YNINLPKEEIGIIAIYFKMSSRRDMSEQKKIGVLLMAHGRVASSVCEVTNKLLGINHA
                     RYLDMPFEKKPEEMIEEATTIIKDIDEGRGVIILVDMGSLSYFGDIISGKTGINIRTI
                     SRFDTLLSIEVIRRAVLPDLTIDEIVDEVKGIEPQVKEKVSKYNNTKVDFRKPIILTL
                     CITGLGGACKIKKILEENIKSITEKVDIEPIGMIEYPSLDLEIKQISNDKNVIAIVGT
                     IDPQIANIPFFSLVDINDKAKLNQINNLIDTNQIGKTNKKYELSDLIHKEDIFVDLQV
                     KSKEEVLKYMSKKLREKGYVTKNFYKKVVERDNLFPTELSGEIAIPHTDGIDVIRPAI
                     SIAILKNPIVWSKSKVKIILLLAVDQKCFNPLSTLLSFIETDEFKKIKNIKDRDFIRE
                     MILDGVNENN"
     gene            26084..26548
                     /locus_tag="KRL67_000523"
     CDS             26084..26548
                     /locus_tag="KRL67_000523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBH1521428.1"
                     /translation="MESMKIISSNLIFKNIEVSNNEDALKFLGQRLFDEQYVKESYIQ
                     AVVAREKKYATGLPTEIYGVAIPHTDIVHVNEPGIAIGILNKPVKFIMMGTDDTEIDV
                     KVVFMLAVKEPQEQLQLLEKLMTIFQDKNILNNIVDLSEESVSDLLNSKLKN"
     gene            26629..26931
                     /locus_tag="KRL67_000524"
     CDS             26629..26931
                     /locus_tag="KRL67_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBH1521429.1"
                     /translation="MGVTNVTKKILVACGTGVCTSTIAINKLKKAFQDIGKLDMVNIT
                     QCKVVEVASKAPDYDLIVCTTQVSSSIKTPVINGLPFLTGVGMDKLINDVLEELEL"
     gene            26993..28357
                     /locus_tag="KRL67_000525"
     CDS             26993..28357
                     /locus_tag="KRL67_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425524.1"
                     /note="with GatAB forms a phosphoenolpyruvate-dependent
                     sugar phosphotransferase transporter for galactitol;
                     subunit IIC forms the translocation channel and contains
                     the substrate binding site; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HBH1521430.1"
                     /translation="MEGLYMEILNFIVGLGAQVMMPIIICIFGLILGTKLGKSLRAGL
                     TVGVGFIGLNTIIALLTDNLGPATQQMVKNLGLSLSIIDVGWPAASAIAFASTVGALI
                     IPIGLVVNIVMLITNTTQTVDVDIWDYWHFAFTGALVAGATQSVMWGVFAAVANMVIV
                     LVMADLTAPGIEEYLGMPGISLPHGFTQAFVPIAIVVNKLLDLIPGINKIEINADTLQ
                     KKFGLFGEPLIMGSVIGVIIGIAAKYDIKGILQLGVTMGAVLILIPKMAALLMEGLLP
                     VSEAAQEFIEKRFKNRGKIYIGLDSAVGIGHPVTLSVALVLVPLTILIAAILPGNKVL
                     PFADLAVIPFALVLIVPITKGNVFRTLIIGIIIITSGLLIATNLAPLFTQMALNASFK
                     MPEGATMISSICDGANPLSWVFVKVMNYKVIGGVVFGVIALGMAIYNRNRIINENKKL
                     SIEE"
     gene            28392..28865
                     /locus_tag="KRL67_000526"
     CDS             28392..28865
                     /locus_tag="KRL67_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH1521431.1"
                     /translation="MKKYKYDSKRYLFSVTIPGFFMILILIYALFNNYINFNLNIYSL
                     LIIVCTYGIFNTFISSSNPKKIIIDSKCIHFTSFMTTHKYEIKDIEKIRIREFYNKKI
                     YLKINDGNLFKGRYWIRTNMFNNSIALYSYFIELEECLYPDSLKFRNKKFENKNI"
     gene            29037..29687
                     /locus_tag="KRL67_000527"
     CDS             29037..29687
                     /locus_tag="KRL67_000527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HBH1521432.1"
                     /translation="MLLDKLRKEVLQASLDLLNYNLVTLTGGNVSGRDEQTGYIAITP
                     SGMDYRNLTPSDIVIVDVDGNIIDGKWKASVDLSDHLYIYKHREDINSIIHTHSTYSS
                     CFAILNEPIECASTTLANEVGGSVPVAKFSPPTSNKMGKCVIEAIGDKRACLLANHGV
                     IAVGPSVGHALTAAVMLEDSAKVYYLAKSIGTPVLLPDEEIQRARDVFFNVYGQDK"
     gene            29863..30795
                     /locus_tag="KRL67_000528"
     CDS             29863..30795
                     /locus_tag="KRL67_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase family protein"
                     /protein_id="HBH1521433.1"
                     /translation="MYYLLNKSTNPYFNLALEEYLFLNDKYNDDIIIIWRNEESIFIG
                     KNQNPYQEVHHDVIEKGEIPILRRISGGGTVYHDLGNINMSFIQKDRQLHEIDFLEHT
                     KFMQKMLSTLGLDVSITERKDLFLNGKKISGSAQSIKRKNSLYHGTLLYDSDLNKLTK
                     YLNSNKATESNATKSVSSKVTNIKFLLEKDINYFLDYCVEYLKSNIKNIKELELDEED
                     IKSVYNIGEDKYKKIEWTYGKTPKFQVVLPFNNESKINIHVNRWKVSKFFISYDDNLI
                     NMDKLLDLNFFKEEIKESILENYPHYIDLINLIF"
     gene            31069..31782
                     /gene="ispD"
                     /locus_tag="KRL67_000529"
     CDS             31069..31782
                     /gene="ispD"
                     /locus_tag="KRL67_000529"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HBH1521434.1"
                     /translation="MYSVIIVAAGSGRRMNLDINKQFIKLREKEIIAHTIQVFYENIN
                     IDEIVVCIKKEEEDFFNENIINKYNFKNIKIAYGGKERQDSIYNGLKKLDKNCDIVLI
                     HDGARPFVDHRIINESIKVAKEKKAVVVGVPVSDTIKIVSDGTVQETPERNLLWAAQT
                     PQTFEYNLIIDAYEQAYKNNYYGTDDSMLVENIGQSVTMVMGSYENIKITSPEDLNIA
                     EQILNMEKRDEVNSRRRII"
     gene            31779..32264
                     /locus_tag="KRL67_000530"
     CDS             31779..32264
                     /locus_tag="KRL67_000530"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HBH1521435.1"
                     /translation="MRIGLGYDVHKLTEDRKLIIGGVEIPHDKGLLGHSDADVLIHAI
                     MDSILGALALGDIGKHFPDTDEEYKGADSMKLLEHVYNLITSKGYKIGNIDSTIIAQS
                     PKMAPYIESMRSNISKVLNTDIDNINIKATTEEGLGFTGAKQGIASQSICLLLLTSQN
                     N"
     misc_binding    32359..32567
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            32631..34349
                     /locus_tag="KRL67_000531"
     CDS             32631..34349
                     /locus_tag="KRL67_000531"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HBH1521436.1"
                     /translation="MKMSKMFMPTLKEIPADAEITSHQLMVRSGMIKKMTSGVYNQLP
                     MGLRVFKKIEQIIREELNKKDCQEILCAALLPSELWKESGRWTAMGEEMFRLKDRTER
                     EYCLGPTHEEAFTDIIRQEITSYKQLPLNLYQIQVKYRDERRPRFGVMRTKTFTMKDA
                     YSFDADDKGLDKSYQDMFDAYVSIFDRCGLENSPVQADSGAIGGSTSAEFMVKSEVGE
                     DEVVFCSGCDYAANVERAESCNLASQKEEMKELEEVHTPGAATIKELEEFLKTSPDKF
                     AKTLVYEADGKTVVVVVRGDREVNEIKVSNAIGSVIEFALATDDVVRKVTNAEVGFAG
                     PIGINADYVFIDKEIVEQRNIVVGANKTEYHIKNANYGRDFEGIVGDFRNVQEGDKCI
                     VCGKPLEIARGVEVGHIFKLGTKYSESMNANFIDKDGKSKPIVMGCYGIGVERTAAAI
                     IEQHNDEKGIIWPLSVAPYHVVIIPANMKNEEQISIAENIYNDLQAMGVEVLLDDRDE
                     RIGVKFNDSELIGIPMRITVGKNINEGKVEFKLRHKEDKEIIDIEEINEKVKAEFIRN
                     NVRLGQ"
     gene            34467..35912
                     /locus_tag="KRL67_000532"
     CDS             34467..35912
                     /locus_tag="KRL67_000532"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HBH1521437.1"
                     /translation="MAKNEKQFVEEITKMEDDFPQWYTDVITKTDLVDYAPVKGFMVV
                     KPYGYALWEKMQEFMDKKFKETGHKNCYFPLLIPESLLNKEAEHVEGFAPEVAWVTHG
                     GNKKLEERLCVRPTSETIICTMYAKWLKSYRELPYLYNQWCSVVRWEKSTRPFLRTSE
                     FLWQEGHTLHETAEEAQEETIQQLEVYKALCEELLAMPVVAGQKSESEKFAGGERTYT
                     IEAMMHDGKALQSGTSHFLGQHFTKAFDITFADREGNLANPYHTSWGASTRLIGGLIM
                     THSDNRGLVLPPRVAPIQVVIVPIAAKKGNVMETVDKIYADLKTKGVAVEVDDRDNYT
                     TGWKFNEWEMKGVPVRVEIGPKDIENNQAMVFRRDTLEKDSMPLEGLADAICDLFDVI
                     HNDMFEKARKHREDNTSIVENMDEFRKALEEKPGFIKTMWCGDAECEAKIKEETGATI
                     RCLPFEQENLGHKCVYCGKEADSMVVMAKAY"
     gene            36353..37834
                     /locus_tag="KRL67_000533"
     CDS             36353..37834
                     /locus_tag="KRL67_000533"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HBH1521438.1"
                     /translation="MNVRVRFAPSPTGFVHIGSLRTALYNYLFAKKMGGEYILRVEDT
                     DQSRLVEGAIENMLNAMKWAGVNHSEGVILDDSGKVVQKGEYGPYIQSQRLDIYQEYI
                     KQLLDSGKAYYCFCTKERLDEVRDAQRAAGETAKYDGHCKNLSKEEVEANIKAGIPYV
                     IRLRLPENHTIKFTDLVRGDMEFNTNDLDDQVLMKTDGFPTYHFAVVVDDYLMKITHV
                     IRGEEWVSSTPKHVYLYEAFGWEAPVFVHLPNILNKEKKKLSKRQGDVAVEDFKKKGY
                     LPEGLVNYVALVGWSPEDNQELFTMKELEKAFSVERVSKSGGVFDTEKLNWVNQHYIK
                     DGDDAYLTDLAIPFLIEDGFITEEEATSKYDFLKSMISVLKEKLQYVKEVTEHASIFF
                     GDKIEVETEEGSEFLRLEHIPTLIDALREKIEKAEVLNAEFVQAMLKEIQKEYKIKGK
                     NLFMGSRIILTGQMHGPDLPKVMEVLGKETCLNRIAYVKNNIL"
     gene            38055..38210
                     /locus_tag="KRL67_000534"
     CDS             38055..38210
                     /locus_tag="KRL67_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH1521439.1"
                     /translation="MYSNIDDVKKELKELCLEYVTILEKLKDEKMITEETFEKCSSQK
                     KIFLEEQ"
     gene            38355..39752
                     /locus_tag="KRL67_000535"
     CDS             38355..39752
                     /locus_tag="KRL67_000535"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HBH1521440.1"
                     /translation="MKVYNTLTRAKEEFVPLEEGKVKMYVCGPTVYNYIHIGNARPFI
                     IFDTLRRYLEYRGYDVTYVQNFTDVDDKIINRSHEEGISPEEVAAKYIKEYFVDCDGL
                     GIKRATVHPQVTDNIQQIIEFIKELEDKGYAYAVNGDVYFDTNKFEGYGKLSGQKQED
                     LEAGARIEVNDQKRHPMDFVLWKAKKEGEPGWDSPWGEGRPGWHIECSVMSKRYLGET
                     IDIHAGGQDLTFPHHENEIAQSEARSGKTFSKYWMHNGYININDEKMSKSKGNFFTVR
                     DISKLYDLEIVRFFMLSAHYRNPVNFSDEMLNQAKAGLERLYNTKEKLEFTLSNLVES
                     PLTEKEIELVKELDNFRQKFIDAMDDDVNTADAVSVIFELAKLINSNVDENSSLEFAK
                     KCLDEFNELTGVLNIVNKKKDTVLDKDIEELIQKRTDAKKNKEFQLADDIRQQLLDMG
                     IVLEDTRQGVKWKRI"
     gene            39737..40150
                     /locus_tag="KRL67_000536"
     CDS             39737..40150
                     /locus_tag="KRL67_000536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mini-ribonuclease 3"
                     /protein_id="HBH1521441.1"
                     /translation="MEKDIKMEKTELVTMSPLVLAYLGDTVYETYIREYLIRQNTQKK
                     VNDLHKLAIKYVKAKAQATIIHEIEIELTEEESKIYKRGRNQKSNTSPKNADIIDYKH
                     ATGFEALVGYLYLNNEIERLQYIINKGIKIIERDM"
     gene            40256..41014
                     /locus_tag="KRL67_000537"
     CDS             40256..41014
                     /locus_tag="KRL67_000537"
                     /EC_number="2.1.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent thymidylate synthase"
                     /protein_id="HBH1521442.1"
                     /translation="MKVKLISHTPEPEKVIAMAAKLCYSPVGTDEIEKDLTDESIEKF
                     LNMLLSIGHGSILEHASFTFSIEGISRACSHQIVRHRIASFSQQSQRYVKLEQFEYII
                     PPEIEKIEKAKELFIDSMKKDQENYDKLVEILFENHYNDLIKNGKNEKTAKRQAEKKA
                     IEDARYVFPNACETKMVFTINARSLFNFFEHRCCERAQWEIRNLAVEMLREVKKVAPI
                     LFKKTGPSCVNGSCPEGTMTCGDIVQVREKFKAL"
     gene            41027..41764
                     /gene="rlmB"
                     /locus_tag="KRL67_000538"
     CDS             41027..41764
                     /gene="rlmB"
                     /locus_tag="KRL67_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HBH1521443.1"
                     /translation="MASIEGRNPVIEAIKSDREIDKILIANSAKEGSIKKIIGMAKDK
                     NIIIQYVDKHKLDEVSTSHSHQGVIAYASEYKYYELDELIDLAKNKDEDPFFIILDEI
                     TDPHNLGSIIRTADAVGAHGVIIPKRRSVHITPVVAKASAGAVEYMPVCKVTNIVNTI
                     KRLKEEGLWIAAADMDGETFYKQNLTGPLGVVIGSEGFGISRLVKQNCDFIVKMPMIG
                     NVTSLNASVAGGILLYEIFRQRLDKSK"
     gene            41767..42294
                     /locus_tag="KRL67_000539"
     CDS             41767..42294
                     /locus_tag="KRL67_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HBH1521444.1"
                     /translation="MKKNINHYLIIDGYNIINAWDNLKELAKEDLEDSREKLIDDIIE
                     FSEFMGYKTIIVFDAYNVKNSREKVEKRKHITIVYTREHQTADSYIEKFITSLSKYDY
                     VKVATNDYAEQQMILGKGATRMSARELKLELDRSKNKMKEKNIGLRKKIQRNWLEERL
                     DEETLSKLENIRRKR"
     gene            42359..43006
                     /gene="sigH"
                     /locus_tag="KRL67_000540"
     CDS             42359..43006
                     /gene="sigH"
                     /locus_tag="KRL67_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sporulation sigma factor SigH"
                     /protein_id="HBH1521445.1"
                     /translation="MLVAKEKSYELVDNCQQDEYNIVLRASEGDKIALEYIITKYRNF
                     VKAKAKSYFLIGADKEDIIQEGMIGLYKAVRDFDGSKTNSFKCFAEICITRQIITAIK
                     TATRQKHIPLNSYVSLNKPIYDEESDRTLLDIIATSIVTDPEELIISKEELKNIESKM
                     NELLSDLELEVLELYLNGKSYQFIADKLKRDVKSIDNALQRVKRKLEKHLENRND"
     gene            43080..>44266
                     /gene="tuf"
                     /locus_tag="KRL67_000541"
     CDS             43080..>44266
                     /gene="tuf"
                     /locus_tag="KRL67_000541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HBH1521446.1"
                     /translation="MAKAKYERTKPHVNIGTIGHVDHGKTTLTAAITKTLYDRYQLGE
                     AVDFANIDKAPEERERGITISTAHVEYETPNRHYAHVDCPGHADYVKNMITGAAQMDG
                     AILVCSATDGPMPQTREHILLSRQVGVPYIVVFLNKCDMVDDEELLELVEMEVRDLLT
                     EYDFPGDDTPIVRGSALMALEDPKSEWGDKIVELFEQIDEYIPAPERDTDKPFLMPVE
                     DVFSITGRGTVATGRVERGVLKVQDEVELVGLTEAPRKVVVTGVEMFRKLLDQAQAGD
                     NIGALLRGVQRNEIERGQVLAKTGSVKAHTKFTAEVYVLKKEEGGRHTPFFDGYRPQF
                     YFRTTDVTGACKLPEGIEMVMPGDNVTMEVDLINSIVVEEGLRFSIREGGRTVASGVV
                     ATI"
BASE COUNT        18115 a         4642 c         8356 g        13153 t
ORIGIN      
        1 caaatgttta gcaataaaga tgagaaggta tatttaaaat ataaagtatt taaagttctg
       61 aaatctaatc atgaagcaaa aaattagcac ttggttatca ataaatataa taaataaaaa
      121 ctcataatta atgaaagaat aaattcatat ctaaatttct ataaaaaatt taaatttata
      181 caatttaggg ctttaaaaat ttaaataata ggtgtaaaat aatagtcaaa gaaagtcaaa
      241 aataaaaagg tgtgtggtga tattaatggc aactatgaca gatataattg aaaaatttat
      301 taaagatttg atggaagaag ataatagcat acaaattcaa agaaatgaac tagcaaactt
      361 gtttagttgt gcaccatcac aaataaatta tgtcttaaca acaagattta caatagatag
      421 aggatattac attgaaagta aaaaaggtgg cggaggatat gttcaaatag aaaaaatacg
      481 aaaaagtaaa gatggacata ttagagaact actaaatgaa aaaataggaa gtcagatatc
      541 ctataaaaaa gcaaaggaat tattagaagg tttaaaagag tcggatttga ttaacgaaag
      601 agaattaaaa ttaatattat atgcaataga tgataaatca ttgtgtatgc caatatatga
      661 attaaaagag aaggtaaggt ctaatatttt aaagaatata ataataggat tatttagtat
      721 agaggagtga taatttatgc tatgtcaaaa atgcaataag aataaagcat ctgtatatta
      781 taataagata gtcaatggag aaaaaacaga gatgtattta tgtagtgagt gcgcaaaaga
      841 gaatacagaa atgaatttta atttagatat gcctttttca atgatggata tattttctaa
      901 tttaggtttt caacctaaaa aagaacttga ggaaaagcta gtttgtccta aatgtaatac
      961 aacatatagt gagtttaaga ataatggaag atttggatgt agtgaatgtt ataatgcatt
     1021 tagtagtcaa gtaaatccaa tgcttcaaaa tatacatggg catatagaac atacaggaaa
     1081 agcacctaaa aaatcttttt ataagataag tgtagaaaat gagattaaag aactaaaaga
     1141 agatttagat agagctataa aaaatgaaga gtatgaatta gcagctcaat ttagagataa
     1201 aattaaatat ttaaaaggtt ctattgacta agattggagt gatattatga aagagaacat
     1261 agtcatgaaa tcaagagtta ggctagctag aaatttaaat aactatccct ttccaaataa
     1321 attagataaa gaatgtgcca tggaaataat agaaaaagta aaaaatgcgt ttattaattc
     1381 taacttagaa caaaaagaag aatttgattt ctataaaatt gaagatttag accaatctaa
     1441 aaaaatgctt atggttgaag aacatataat aagcccagat ttagcagaaa atgataaaag
     1501 tgcagttata gtaaaaaaag ataaaactat aagcataatg ataaatgaag aagaccatat
     1561 aaggatacaa actatatgtg atgacttaaa cttagaatat gcatatagtg tagcaaatga
     1621 aatagatgat ttgttagaat caagtttaga atatgcattt aacacaaagc taggttatct
     1681 aacatcttgt ccaactaaca caggaacagg aatgagagct tctgtaatga tgcatttacc
     1741 agcattaagt cagctaggtt atatggatga actttataaa atttcatcac aaataggtat
     1801 agcaattaga gggatatatg gagaacgaac agaagcatta ggaaatatat atcaaatatc
     1861 taatcaactt acacttggaa gaacagagtc taatattata gaaaatgtaa gtggacttac
     1921 aaaagatgca atttcaaaag aaataaaagc tagagaaata ttgcaaaaaa aattaggtat
     1981 aaaactagaa gataaaatat ttagatcgat agggacactt gaaaattcaa gagtaatgag
     2041 tagcgcagag gcaatgagcc atctatcaaa tataaaaatg ggaatagaaa tgaactatat
     2101 agataaacta gatttaaagg ctatagaaca acttatgata ggtatacaac cagcacatca
     2161 gtcaataatg tataagagtg atgatgttga gaatagagat ataaatagag caacatatat
     2221 tagagaaact ttggaaaaat taagaggagg gatgaattgt gaacttcaat agatttacac
     2281 aaagagctaa aaaggcaatt gatttagcgt ttgaatctgc taaaagtcta ggacacaata
     2341 ttgtcggaag tgaacatata cttttaggtc ttttaagaga agaagaaggt atagccgcta
     2401 aagttctaag taaagttgga tttacagaag cttatttaga aggcaaaata gttgatatgg
     2461 aaggtaaggg agaagaaatc tcagaagaca tagtattaag tccaaggagt aaacaaatac
     2521 ttgaattatc aggtatgttt gcaaataaat taaaaacaaa ttatattggg actgagcata
     2581 tattattagc aattattcaa gaaggtgaag gaatagctaa taagatttta aattatgctg
     2641 gagtaaatga taggacttta gcccaattaa caattgatat gatgggtatt agtgataaaa
     2701 atcaatacaa agcagaaaat agttacacgg gcaaccagaa tcaagcagaa tctaaagtac
     2761 ttgataagta tggaagaaat cttacactat atgcaaaaca gaataaaata gaccctgtaa
     2821 taggtagaga aaaagaaata caaagagtaa tacaaatatt aagtagaaga actaaaaaca
     2881 atccagtgtt gataggagac ccaggcgtag ggaaaacagc catagctgaa ggtctggcta
     2941 caaatatagc cttaggaaat gttccagaaa cactaaaaaa caaaacttta tactctttag
     3001 agatgggttc attattggct ggggctaagt atagaggaga atttgaagaa agaattaaag
     3061 aagttgtaga tgaagttgtt aaaaatggaa acataatctt atttatagat gagatgcata
     3121 cgataatagg ggctgggtct actggagaag gctctataga tgcttcaaat atattaaagc
     3181 cagccttagc aagaggagaa atacaagtta taggtgcaac tacaattgat gaatatagaa
     3241 agcatgttga aaaagattct gctctagaaa gaagattcca accagttatg gtagatgaac
     3301 caactaaaga ggactcaata aaaatattag aaggattaag agataaatat gaagctcatc
     3361 ataaagttaa aataactgat gatgctataa aggcagctgt agaattatca actagatata
     3421 tatcagacag atatcttcca gataaagcaa tagacttgat tgatgaagct gcatcaaaag
     3481 taagattaaa agaaaataca cctccttcag aaataaaaaa attagaacta gaaatagaaa
     3541 atatagataa agaaaaagaa gaagcagtaa gatgtcaaga ctttgagaaa gcagccaaaa
     3601 taagagatga acaaggtcta cttaaaaaac aattagaaga tgttagagaa agatggaaca
     3661 agtcatctaa acattcagat ttagttgatg gagaagttat tgctgaagta gtgggcttat
     3721 ggacaggaat accagttaat aaaattcttg aagaagaggc tgatagactt ttgagacttg
     3781 aggaaatact gcacaataga gttataggtc aagaacaagc agtaaaatct atctcaaagg
     3841 caatcagaag gtcaagagca ggtcttaaag accctaatag accaattggt tcatttttat
     3901 ttttaggtcc aacaggagta ggtaaaacag agttatctaa ggcattagca gaagtacaat
     3961 ttggagatga aaatcaaata attagaatag atatgtctga atatatggaa aaacatgctg
     4021 tgtcaagaat gataggttca cctccaggtt atgtaggtca tgatgaaggt ggtcaattaa
     4081 ctgaaaaagt aagaagaaat ccatattcag ttatcttatt tgatgagata gaaaaagctc
     4141 atccagatgt atttaatatt ctattacaaa ttctagatga tggtagactg actgattcaa
     4201 aaggaagaac tgtagatttt aagaatacta ttgtaataat gacatcaaac gttggagcat
     4261 ctacaattgg tagacaaaaa actttaggat ttagtatagc taaaggagac gaagaagaaa
     4321 aatctcaata tgaaaaaatg aaagaaaata taatgggtga attaaaacaa agattcagac
     4381 cagagttttt aaacagaata gatgatataa ttgttttcca ctcattaaat gaaaatcata
     4441 tatcaaaaat agtactttta atggcagcga aactacaaga gagattaaaa gaaatggata
     4501 taaaattaga aatgagtgat gaggctgtta aattaatttc taaatctgga tttgacttag
     4561 aatatggagc aagacctctt aaaagagctt tacaaaaaga gttggaagat gagctatctg
     4621 aagcaatctt aaaaggtaat gtgaaaaagg gaagtaatgt agtagcaaaa gtaaaagatg
     4681 aaaaaatagt ttttgaaact aaataattta atagagttgc attttaaaat attgcaaata
     4741 taagttttat aatctaaaca aaattttaag ggcaatgaat ctataataat tcattgtcct
     4801 taattatttt atttaatctt tacatattgt ctttcagata tgctaaaatt atagtataga
     4861 ataaaaaatt aaggatgata aaagtatggc aaaaataaaa acaaaatatg tatgtcaatc
     4921 ttgtggatat gaaacagcta agtggcttgg taaatgtcca gaatgtacaa aatggaatac
     4981 atttgtagaa gaaatagacc aaaaaagcac caaaaaagag gtctttataa tagataaatc
     5041 ttcatctaaa ccagtcagta taaactcaat agaaagtaaa gaagaagaga ggtttacaac
     5101 agatataaac gaattagata gagtgcttgg aggaggtata gttaaaggct ctttagtgct
     5161 tgttggtggt gaccctggta taggtaaatc aacactttta atccaagtat ctagtaatgt
     5221 tgccaattta ggaaaaactg ttttatatat aactggagaa gaatcagaat ctcaaataaa
     5281 aatgagagct aaaagattag gtattaactc agaaaattta tatatatttg ctgaaaataa
     5341 tttaagtata attgaatctt atttagaaag tgtaaatcca gaattaataa ttctagactc
     5401 aattcaaact gtcttcagtc cagaaatatc ctcggcacca ggaactgtca gccaaattaa
     5461 agaaggtact tcaaaattca tgaaaatttc taaaaaaatg ggaatttcaa cgttcatagt
     5521 tgggcatgtt acaaaagaag gttcactagc aggaccaaaa ttattagagc atatggttga
     5581 tactgttcta tattttgaag gagaaagata taatacttat agattagtga gagctgttaa
     5641 aaatcgattt ggctcaacta atgaacttgg agtatttgaa atgagggatt taggattagt
     5701 agaacttgac aatccatcta aaattttaat ctctgaaaaa ccaaaagatg tagcaggttc
     5761 agtaataata tctactgtag aaggtacgag accaatgtta cttgaattgc aagcattagt
     5821 ttctcctaca agttttggta taccaaaaag aacatctaca ggagtcgatt acaatagagt
     5881 aggtatgtta ttagcagtat tagaaaaacg tgttggtttg caaatacaaa atcaagatgt
     5941 ctatataaac atagttggag gaataaagat aaatgaacct tcaattgatt tagggatagc
     6001 catttctgta gcatctagtt ttagaaatat acctatagat gaagatatag ctgttacagg
     6061 agaagtggga cttacaggag aagtcagagc agtaagcttt atagaaaaga gaatagcaga
     6121 gtgtaaaaaa cttggattta aaaagatagt agttcctaga agtaattatg atgttgtaaa
     6181 agagaccaaa ggcatagaaa tgtggccagt agataactta agacaagcta taaatatagt
     6241 acttgggagg aatcaataaa tatggagaat tttctagata ataaaaatat gctatatgca
     6301 ttaaaaatga tatctcctgg aactccactt agattaggtt taaacaatgt actaagagct
     6361 aagactggtg gactaattgt aattgcaaca aacgaagatg taatgaaaat agtagatgga
     6421 ggatttgcta taaatgcaga atattcacca tcatatctat atgaattagc taaaatggat
     6481 ggagctatag ttttaagtgg tgatgtaaag aaaatattat ttgctaatgc acaacttata
     6541 cctgactatt ttatagaaac atcagagacg ggaacaagac atagaacagc agaaagagta
     6601 gcaaaacaaa ctggtgctat agtcatagga atttcacaaa gaagaaatgt tataacagtt
     6661 tatagaggaa atgagaagta tgtagtcgaa gatatatcta agatatttac taaggcaaat
     6721 caggctatac aaactctgga aaaatataag acagtattgg accaagctat aacaaattta
     6781 aatgccttag agtttaatga tttggtaact atttatgatg ttgcattagt catgcaaaag
     6841 atggaaatgg taatgagagt tacaagtata attgaaaaat atgtgataga attgggtgat
     6901 gaaggaactt tagtaagtat gcaattagaa gaattaatgg gtacgaccag aatagaccag
     6961 aaattaatat tcaaagatta taataaagaa aacacagaaa taaaagaact tatgaaaaag
     7021 gtcaaaaatt taaattcaga agaactaata gaattggtca atatggcaaa actattaggg
     7081 tatagtggtt tttcagaaag tatggatatg cctataaaaa caagaggtta taggattctt
     7141 agtaaaatac atagactacc aacagccata atagaaaact tagtaaatta ttttgaaaac
     7201 tttcaacaaa ttttagatgc atctattgaa gaattagatg aggttgaagg aataggtgaa
     7261 ataagagcaa catatataaa aaatggactc ataaaaatga aacaattagt cttattagat
     7321 agacacatat gatgaatttt aaatgaattt tactactttt ttcttgaata taatatacac
     7381 tttggtctat aatagataag tataactagg gggtgaagca ttgataagga aagtaacaag
     7441 aatattgttt attttacttg gatttacaat aggtataaca acctatttaa cattaatgaa
     7501 agactttgaa atattaacat ttggaaaaga aacatatggt tatatcgcag ctgtagtagc
     7561 aggtataata atagccattt taggatatct tatagaacca tgggttgtaa ataaagtaaa
     7621 agaaattgct aaaatagtgg ataaagaatt gtcaaaatat cctcagactg atatattgtt
     7681 agggtctatg ggtcttatag taggatttgt tattgcgtat ttattaagtg gattagtaaa
     7741 tagaatacca atagtaggtg gaatactatc cttactatta tatttattcc taggatatct
     7801 tggaatgaaa gttgcgctta aaagtaaaaa tgatttattt aatattggaa aactaggaag
     7861 attagcaaat cctattaagg ataaagataa ggataaagaa aataaaaaag aagtaaaagc
     7921 cattcctcca aaagtattag atacaagtgt aataattgat ggaagaatag ctgatatttg
     7981 taagactggg tttatagaag gaaaacttat tattcctgct tttgttttgg aggaactaag
     8041 acatattgca gattcttcag atgatttaaa aagagtaaga ggtagaagag gtctggatat
     8101 attaaatata attcagaaag aattaaatat agaagttgaa ataagtgaga gagactttga
     8161 tgatatagca gaagttgata gtaaattatt aaagcttgct caggttttaa atggaaaagt
     8221 tgttacaaat gattataact taaataaagt tgctcaattc caaggtgttg aagtattaaa
     8281 tataaatgag ttagctaatg ctataaaacc agttgctata ccaggtgaag atatggttgt
     8341 tcaagtggtt aaagaaggaa aagaggctca acaaggtgtt gcatatctag atgatggaac
     8401 tatgatagtt gttgatggag gaagaaaata tatgaatgag actataaaag tactcgttac
     8461 ttctgttctt caaactcctg caggtagaat gatatttgca aaacctaaaa attaatatgt
     8521 tcaaagaaac ctataatttg ggctgtttat tttaaataga tggtccaaat ttatttattt
     8581 ataacgataa tgtttaatgt attcttaaag atggggattt aaatcagaaa gcagggagaa
     8641 tttatgagaa aaaagattat atctttctta gcaacattta tcataatatt aacatcagca
     8701 agtcagtata gttttgcaga tgatatatca acacgtggaa aagtaatatt tatagatatg
     8761 aatagaacta gtatgagtaa tatgttaaga ataaaatcat taagagaaga actagacaat
     8821 agagggtata ttggcttgat gaatataaga ggcgataaag gaagtgatga tagaaggtca
     8881 tatgctagta tgggtgctgg tggtagagct aatgtggcta atgaagaaga tattaatttt
     8941 gaaagctcga gtaaggatag agatatagtt tttgaatcag ctacaggaaa aagtgcaaaa
     9001 ggtataaata atttaactat aaataagtct ataaatgaaa atttaaactt tggagagtat
     9061 ggttctgtat tagggtcatt aggacaaagt ttatctgata atggattaaa agcatctgta
     9121 ttgggaaatt cagatataat tgaaaatggc caacttataa aaaacagaaa cttatgtcta
     9181 actgcaatgg atgaatatgg aagaatacct aatggaaatg tagatactat aaataaaaaa
     9241 gacttatcta tgccttatgg aatatcaact gattatgata aattgattgt agaaaccaaa
     9301 gaagcttaca aaaataatga tgtggttttt gtggagcttg gagatacata tagattagat
     9361 ttatataaac caaatttaaa tgaaaaagca tatgagagta tgaaagataa catagaaaaa
     9421 aatatagatg tatatttaaa aaatatattt tctattgttg gagaaaatga cactgtctac
     9481 atagcaagtg catttccaag tgatttagat tataaaaata aaagaagact ctctccaata
     9541 ataaaattaa atggagaagg gaaaggtata ttatcatctt caactactag aagagaaggt
     9601 attgtaacaa atttagatgt tggggttgaa atattagaca actttaatat aaaaaatcaa
     9661 aatatggttg gaagaaaata tgaacttatc aacagagatg ataataaaga atttttgatg
     9721 gatgaatatc aaaagatagt atctatttca agcataaggt ctacaatact gaatggtttt
     9781 gtaagcatag ttttcttgtc atggatagtt gctatgattg caattttatt tagaaagcat
     9841 atatcaaaaa attataaaga aacaacattt tttatattaa aggaactttt aaaaatagga
     9901 atagtaatgc cattgtcatt tatgataaca ccaataatga atttcaaaac accattagca
     9961 atatctttag gaataattat aataacactt actatatatc ttataagtaa agttttaata
    10021 aagaacgatt taaaaaacat gctgtttttt actggattaa ccatagtaat tatggttatt
    10081 gatgctggat ttggctcata tttaatgaaa agcaatgtta tgagttatga ttgtataatt
    10141 ggagctagat attatggagt aggaaatgag tatcaaggtg tggcaattgg tagtgcaata
    10201 tttacatttg caattttact tacatataaa aatataccaa agtggtctgt aatagttttt
    10261 tctttgctta tcttaattac aagtgcatct ccaattatgg gagcaaatgt tggaagtgct
    10321 atttctgaat gtgttgcatt tttactattt atactattaa tatataatgt gaagatagac
    10381 ttcaaaaaaa ttgtattatt aggtatagca gtattatttg tattaggagc ttttgtagca
    10441 atagatatga ttttgggaag taattcacat ttaggtatgt ttgtaaaaga aatttacttt
    10501 aatggaccag gagaaataat acaaacattt agtagaaaga tagagatgaa tttaaagcta
    10561 gctcaaacca gtgcatgggt aaatatactg ttaactggaa taggggtgat tttagctctt
    10621 atgattaatc agattagata ctttaggcaa ctgatggatg agtatccaat tgtatttaaa
    10681 ggatttatag catcaatagc aggatgttta gtaactcttt tagtgaatga ctctgggatt
    10741 gtgtcatcag ctacagcctt tatttatgta attgtaccta tgataacatt aagtataaat
    10801 ctcatagcat taaaagaata agtgctgtat gaatactatg taaatatgaa cacattaatt
    10861 taacttcctt gtttacaaaa taaagaagaa agtttataaa agataaatta taataaaaaa
    10921 gagtttatct taaaattgat ttttctaatc aaattttaag gtaaactttt tttattataa
    10981 agataaataa catataatct catattttag gtagattttt ttcttatatg tttaggagtg
    11041 catttaaatt tttctttaaa aattctgtaa aataatttcg tattagtaaa tccatgagct
    11101 tctaatatac tgcataaggt atcatttgtg ttaattaagt ctttataaat atgagaaata
    11161 cgcacatcat ttaaatactc taagtaagtt tgtcccatat actgtttaaa ttttctacaa
    11221 aaatattctg gttgtaagtg tgctacctta gcgattttgt taatagaaat aggcatttta
    11281 taattaatct ttgtatattc taaaactggc tcaaattttg ataaatcaag tactgttttt
    11341 ttcatatttt tattacctac agatatttta aagtttgtat ataattcaaa taaaagttca
    11401 aataataagc ttgtaaagag cagattagct gcaggaggag aactaagttt aatttctctc
    11461 atgttttcta atataagttt aactttactt gaattttttt tatagttatt gtcattggca
    11521 ttaaccttaa agtcaaaata gcaattttta aaatcaggta tatatttatt tagaaaagac
    11581 aatggtactt gcaataatat agaggtattt tgataagtac atctagttga gtgaataatt
    11641 ccagaattaa tgaacataca ctcgttagct tttaaattat aggtgtaacc ttccatgtta
    11701 atttgtaaat cgccagaagt tatataaatt atttctaatg agttatgcca atgatttgct
    11761 acataactac cactatcatc aaatagttta aaattaacat ctaaattatc aataggttca
    11821 actatttcat gtgaaaaatt atttctgttc ataataatct cctccttata attttaaatt
    11881 ataaatagta aatttataat attcaatatt aaaaagataa tatagactaa taaattagac
    11941 aataaacata cttaagaaaa cggataaaat caaatataat aactgtatat taaaataact
    12001 taaaaaaatt attttattat aaatgcaaaa tataccattt aaataagata agaggggagg
    12061 actttatgtg tatataaaag gtgtaaattt aggaggttgg ttagttttag aaaaatggat
    12121 gacctcaagt ttatttgaag gcacagaagc tgaagatgaa tactatttac caagacaact
    12181 ttcaagagag gtatatgaat ctagaattaa gactcatcgt agtgaataca tcacagagag
    12241 agattttgca acgattaaat ctatgggatt taattcagtt cgtataccag taccatattt
    12301 tatttttgga gattgtgaac catttatagg atgtgttaaa gaacttgata aagcatttgt
    12361 ttgggcagat aagtatggac ttagtatatt gatagactta catacagttc caggaagcca
    12421 aaatggattt gataatggag gaatatcagg tgtatgtatc tggtcacaaa atccagaata
    12481 tgtagggttt acattaaatg tattagaaag acttgcaaga cgttatggga tgagacatga
    12541 attgtatgga atccaaattt taaatgagcc tataacagaa agaatgtgga acataatgaa
    12601 tgttccaaat cgatttaagg ctgcagataa ggagatggca agggggagta aacctaattc
    12661 attagagttt ttacgtgatt tttatataaa agcatataaa gtaatgcgtc cttatatgag
    12721 agaggaaaac gttatagtat ttcatgatgc atttgaatta aaagcatgga aagactttat
    12781 gagagaggaa gaatttaaaa atgttgttct agatacacac caatatttga tgttagctga
    12841 agcagatgga tgtgaacaaa gtattgatag ctatctcaaa tatattagag aaaattatgc
    12901 taaagatatt ttacaagtgc aaaaatattt tcctgttata tgtggggagt ggagcttatt
    12961 taattcatat gcttgtggta tagatacaga tggaggtcaa tcgcctttaa atgggattga
    13021 atcaaatata gataaattat caaaagacga gaaaagagaa ttatatagga aaatagcaaa
    13081 agcacagctt gatgcatgga gaaatggaag tggtcactac tattggaatt ataagttatt
    13141 actagataca gttaatgaag aaggatggat aggatgggat agttgggacc taggcaaatg
    13201 tgtagctcaa gaatggtatc ctatagaata ttaaactaaa attggaggaa aagatatgat
    13261 taaaaaccct atacttccag gttttaatcc agatccttgt atttgtagaa agggagatga
    13321 ttattatctt gtagtttcat cttttgaatg gtttccaggt atacctgttt atcactctaa
    13381 agacttaaaa aattgggagc tatatactca tattttaaca gatgaaacaa aaatagattt
    13441 gaagaagctt ccttcatcta aaggaatttg ggcaccttgt ttgacttatt gtgaagaaga
    13501 agatttattt tacatagttt atggaattat gaactctatg aatgcaagat attttgatgt
    13561 tgataattat ttaattacat caaaagatat aaagggagag tggagtgaac cagtatatct
    13621 acactcatca ggttttgatg cgtctatttt tcatgatgat gatggtaaaa agtggattac
    13681 atcattagac tgggagacta gagagggata tgaaaaacct ggtgttatat gtttagtcga
    13741 atactgtaca aaaaagaaag aaatagtagg atatccaaag agaatatggt caggtggaac
    13801 tgatagaggt tgtattgaag caccacatat aacaaagcgt ggagattact attatataat
    13861 gtgtgcagaa ggtggaactg gatatggcca tagtgtaact atgggaagag ctaaaaatat
    13921 atggggacct tatgaaaaag attcaatgaa tccaattgta acttcaattc caggtgattt
    13981 ttatgaaaga catgaccctg accatctaaa gcctaaatac tataatccag aatcaaaact
    14041 tcaaaaatct ggtcatggaa gctatataga aacaacatca ggtgaagtat atttagttca
    14101 tctaacatct agaccatttg taccagagtt aaggtgtact ttaggtagag aaacagctat
    14161 tcaaaaaatg aaatggacaa aggataattg gcttcgaatg gaagatgaaa gtaatttggc
    14221 aaaagaatac gtatcagaaa gtaaactaga agaacattta gtttcatcta taccaagttt
    14281 tgatgacttt aattcaaatg aattaggttt acaatactat gctcctagga tttcaccatt
    14341 atcatttgca gatgttaagt ctagaccagg atatgtaagg atcagaggac aagaatcaag
    14401 aacatcatta aataaagtga gtatattggc aagaaaatta acttcagtat atgcaagaat
    14461 aaccactaag atggagtttt acccagaggt acatcagcat agtgcgggtt tgataatgta
    14521 ttatgataat atgaattata tcaatttaag aaagtattat agtgaaacat tgggtcaaag
    14581 tgcactatca ataatacatc ttgaaaatgg agagaagacg gaatttttaa atactagaac
    14641 accaataaaa gatataccaa tatatcttcg tttgtatata caaggtcgta agtcatattt
    14701 tgaatggagt tatgatgaaa aaaactacca aagaatagga aaggtatttg atacaactaa
    14761 gttttcagat gagtactgta aatatggaga atttacaggt acgtttatag gattaacttg
    14821 tgcagatcgt gtaaagcata aacactatgc tgattttgat ttttttgaat atatagcaga
    14881 tgaatcaaaa gatgtcgatt aataaaatca ttactaaaat tttcagagca aaggtaagtc
    14941 ttatgctctg aaataagaaa cattagaagg taaatcttac aaaaaggagt atggtattat
    15001 gaacacagaa gttaatgtta aagcaactac tattagatat gaaaagattt cattttttga
    15061 aagaatttct tatggttgtg gggatttggg atgcaatatc atatactcag ctatgtcagc
    15121 atttttgttg ttttattaca ctaattatgc agaggttagt gcagcagctg taggtagtat
    15181 tatgcttgta tctaggatac tggatgggtt tagtgactta acaatgggaa ttatagtaga
    15241 tagaactaaa tccaaatacg gtaaagctag gccttggatt ttgagaatgg cgattccttt
    15301 tgctatagca gcaatattat tattttctgt tccatcaaat cttggtatca cttctaaatt
    15361 aatttatatt tttattacat ataacttagt ttctactgtt atttatacag caatcaatgt
    15421 accctatgca actctaaatt cacttataac tcaagaccag tatgaaagag gagtattaag
    15481 tatatttaga atgatattag ccacttgtgg taccttacta ataaatggac ttacactacc
    15541 tctagttgaa tactttggaa ataatttatc agcatggact aaaacattct ttgtatttgg
    15601 aatagcatct ataatggtat tttttataac atttactgga accaaggaaa gagtaaaagc
    15661 agttaagcaa gataggaatg aagtaatacc atttaaaata ggtattaaat ctttatttag
    15721 aaataagtac tggatacaaa taacattatg tttagtttgc atatttattg tatttgcgat
    15781 aaatggtggt tcatcagtat attatgcaaa gtttatttta ggagatgaaa aattatttgg
    15841 tcctataaat atggtttcaa atatatccca aattatagct atgtttatgg ttgctccttt
    15901 tataaaaaaa tttggaaaaa gaaatgtatt aatagtaggt tcaataatat taatatcgtc
    15961 aaatgttatg tttataatag ttggacaaaa ctatattgga gtaatatttg caagtgttat
    16021 aaagggaata ggaagtgctg gaatagcatc aacaatgttt gctattgttt cagatactat
    16081 tgaatatgga gaatggaaga ctggatatag aactgagggt cttataaata gtgcatctag
    16141 ttttgggttt aaagtgggaa atgggttagg ttcagcgata ttaggtgcag tattatcaat
    16201 tggtggttat gtaggaacat cagctactca aagtgattta gctatagtat caataaaagt
    16261 atgttttata tacctaccta tatttataac agtattacaa attatcataa tgtcttttta
    16321 taagcttgat aaagaatata gtactatact taatgaatta aatactaaat aatgaaaaag
    16381 tttcttataa agtcttaagt ttaattaagg tctattgtaa attttcttta taaaaaaact
    16441 attatatatt tataatatat tggagaattt ttagagaagc gaataataaa aatataagaa
    16501 gacctcaaat gatgaaactg ttggtttttt agagatgatt taataagtta tcgttattta
    16561 aagcattata agtggacaat taattttgag tttatggaga tgatatttaa caattaaaag
    16621 taatatataa tatgcattaa gaagcaattt ttcaactgtt tatgaagatg gtggtacaag
    16681 aaaagtatca cctttttttt gttatacaaa aaaacagttg actttttata tgaacccctt
    16741 ctataatgtt actgaaatta agtaaaatga ttgaacaaat gtaaagatga ttaaaaagct
    16801 tgattttgga ggaaagcaaa tgaaaaaaat tggagactta agattaatga tgaagtgttg
    16861 ttcgctttat tacgaagata atttgaatca acaggaaata gcaaatcaat tgggaatatc
    16921 aagaccaaca atatcaagaa tattaaaaga agcttttgaa caaggaatag taaaaataca
    16981 gatagttgat gttttgaaaa atgattacca aaaaatagag agaagtttgg agagaaaata
    17041 taaattaaaa gaagtaatag tagtagatga taaacaggat gcactaacac aaaaacaaga
    17101 actagcaaga gctgtatctg aatatttaac tagagtagta aaggaaaatg atataatagg
    17161 tgtttcaata ggaacaacac taaaagaaat accaagatat gtggaaaaaa gtaactgtaa
    17221 aaatgttaca tttatacctc tattaggagg gattggagac aatgaaatag atattcatgc
    17281 aaatcaaata gcagtaagtt tggcgagggc ttttggaggt gattttaaat tattacatgc
    17341 tccagctgtt atgtctgact tgtctacaaa agagaaactt tgcaaagacg aaaaaattaa
    17401 agaagtacta gatttaatag ataaaacaac tatagcaatt gtgggtatag gaaatcctat
    17461 gagtttaaac tcaacaataa tggcttctgg atacatgaat gaagcagata tagaagattt
    17521 aaaaaaatac aactcaatag gtgcaatttg tttacaagcc tttgataaag aaggaaaaac
    17581 ttctatactt gaatttaatc aaagagtatt aggagtgaaa ctggaagatt taaaaaagat
    17641 aaaaagaaca ataggtgtcg cttcaggaga tgaaaaaata gaggcaataa aggcatcatt
    17701 aaaagcgaaa tttataaata gtttagcaat taatcacagt ttagcactta aattattaga
    17761 agattgtgac taatagaaag atagataaaa gttaacagga gggaatgtat atgtcaatat
    17821 caaaagaaac attgcttgaa atgtataaga gaatgaatca ggctagaaaa tttgaagaga
    17881 aagttagttg gttttttgca aggggtatgg ttcatggaac aactcactta tctgtaggtc
    17941 aagaagcgtc aagtgtggcg gctgtaatgg ctttagaaaa aggagattta gtatcattaa
    18001 ctcatagagg acatagtcaa tttataggaa tgggaataga cctaaataaa atgatggctg
    18061 aacttatggg taaagaaact ggattttgta agggaaaagg aggctcaatg catatagcag
    18121 atattgagtc aggtaactta ggagcaaatg gagttgtagg tggaggtctt acaatagctc
    18181 caggagctgc acttacacaa cagtataaaa aaacaggtaa aatagtttta tgtagttttg
    18241 gtgatggagc atcaaatgaa ggtacttttc atgaaggaat aaatttatct tcaatatgga
    18301 aattgccaat aatattttac tgtgagaata atttatacgg tatgtcaaca agcataaaac
    18361 gtcatatgaa tatagaaaat attgccacta gagcagcctc ttatggaata gaaggaatat
    18421 caatagatgg atataaccca atagaagtat acgaaacagt acaaaaagct gctgagaagt
    18481 gtagaagagg agaaggacct gtattaattg aaagtagaac ttatagatgg ttaggtcatt
    18541 caaaatcaga tgcaaatgta tatagaacaa aagaagaaat agaatcatgg aaagcaaaag
    18601 accctattga atttttaaaa aattatctta tagaaaataa tctatctaat gaagatgaat
    18661 tagataagat tcaagaattt gcaaagcaat caatagaaga tgcagtagag tttgctcaaa
    18721 atagcccaaa tccaaaaata gagtctttat tagaggatgt atatgcagac taagaatggt
    18781 aaaggaggaa aataaaaatg agtacaaggg aattaacata tgcacaagca ataaaggaag
    18841 caatgtcaga agaaatgaga agagatgaaa atgtaatatt tatgggagaa gatataggaa
    18901 tatatggagg agcatttggt gtatcagttg gtatgataga tgaatttgga ccagaaagag
    18961 taagggatac tcctatatca gaagcagcta tagcaggagc ggcagcaggt gcagcagcaa
    19021 ctggtttaag acctattatg gaagtaatgt ttatggactt tgtaacaatt tctatggatg
    19081 caattgtaaa tcaagcggca aaaatgagat atatgtttgg aggaaaagca caagttccaa
    19141 tggttgttag atgtccaggt ggttctggaa ctggttcagc agagcaacat tcacaaagtt
    19201 tagaagcttg gttttgccat gtaccaggag taaaagtagt tgcaccttct acaccagcag
    19261 atgctaaggg attgttgaaa gcagctatac gagataataa tccagtcata tttgttgaga
    19321 ataaattgtt atacagaaaa aaaggtgttg tgccagaaga tgattatgta attgagattg
    19381 gaaaggcaga tattaaaaga gagggtactg atgttacagt aatcacttat ggaagaatgt
    19441 tacaaagtgt tgaagaagca gcagagactc tatcaaaaga aaatataaat gttgaaatta
    19501 tagatttaag aactttatat ccattagata aagagactat agtgaagagt gtatgtaaaa
    19561 ctggaagagt tttaatttgc catgaagcgg ctaagactgg tgggctaggt ggagaaatat
    19621 cagccctgat aacagaaagt gaatcatttg attatttaga tgctccagta aaaagaattt
    19681 gtggaaaaga tgtgccaata ccatataatc cagatttaga aaaagcagtt gtcccaagag
    19741 ttgatgaaat agaagaagca ataaaatctt taattgttag ataaggaggg atagatatgg
    19801 tggcaaataa aataaaagcc acaccagcag ctagaagtca ggcaagaaaa gacaatataa
    19861 aattagatag gctgattgga agtggagaaa atggaaggat tcatttagtg gatgtgttaa
    19921 attatttaaa agataataaa gcaaatacga ctcctttggc tagaagaata gcagaagatt
    19981 taaatataga tttagaaact attgtaggta ctggatataa tggaaagata agaaagtgtg
    20041 atgtggaaaa actaactgca aaagaaacta ttgtatcaac taatacatct aaatctaatg
    20101 agaaaaaaga gatgaaaatt gaaaatgaaa attcaagcat gtttaacaca gtagaaggaa
    20161 tatttgaaaa gccaaatcca atgagagcta ctgtagcaaa acgaatgtca gaaagttatt
    20221 ttagtgctcc tgtatttaca ttcaatatag aagtagatgc tacagaattg aaggcactta
    20281 gagcaaagtt aatggacaca gtaaaagaat caactggagt aaaattaact atgacagatt
    20341 taatagtaat ggcagtatct aaaatacttc caaatcatca agccttaaac tcagcatgga
    20401 cagacgaggg tatatttaga tataaagatg taaatatagc tattgcagtt ggtttagatg
    20461 aaggtttata tgttccggta gtaaaaaatg caaacaaaaa gtctttaaaa gaaatagcaa
    20521 aagaaagcaa agaacttgcg gaaaaagtaa agacagggaa acttatgcca gcagaccaag
    20581 aaggtaatac atttacaata agtaatgttg gaatgtatgg tataactaca tttacaccta
    20641 taataaatat gccttcaagt gcaatcttag gggtaggtgc tacacaagat aagtttgttc
    20701 cagtaaatgg agaggctaaa ataaaaccta taatgaactt atcattaaca tcagaccata
    20761 gagtaataga tggaacagta gctgcaaaat tcttaaaaga tttaaaagaa ttgttagaaa
    20821 accctttatc aatgcttgta taggttttga tatttcgtta ctaatataaa tagtttgaat
    20881 agattacatt attataattg tttcagtaaa tttaaagctt ttatatatta agaattattt
    20941 ttaacagtaa ctatatgtga atatgatttg cttaattaaa atatatgaaa ataaatagga
    21001 ggagagccta tgtcagtaga agtaataatg ccaaaagcag gagtagccat ggaagaaggt
    21061 actatagtat cttggctaaa gcaagaaggc gaagaagtaa agattggaga acctatacta
    21121 gaaataacaa cagataaagt taatatggag atagagtcag aaggagaagg tacacttgct
    21181 gtaatcatac ataaagagga aggggaagta ttaccagtat ttacagtaat aggtgtaata
    21241 gcagaaaaag gggaaaatca agaggaagta aaagctaaat attcatctgg aaatatttca
    21301 aaagaagata tagttgaaga gaatcaaaat acagaagtta aagaagaaaa aataaacaaa
    21361 aaagagtgta atcatgatta tgatgtagtg gtaataggtg gagggcctgg gggatattta
    21421 tcagcattaa aagcagctct tttaggtggc agagttgctt tagttgaaga aaatatacta
    21481 ggaggtactt gcttaaatag aggttgcatc cctacaaaga cttatataaa aacagcagaa
    21541 atattagaag aaattgacca gttatcaaaa agaggcgtaa aagtaactgt agataaagaa
    21601 caggacatta agaaggccat aaaatataaa aatagagtag ttaaaaaact tacagctggt
    21661 gttggcggac tattaaaaag tagagatgta gaggtgttta atcttaaagc tagtgttaaa
    21721 gaagagcata aagtaatttt atcagatgga aaagttttag atacagaaaa tataataatt
    21781 gctacaggtt ccaaagtaag gatattacct ataaaaggaa tagaatcaaa tttaataata
    21841 actagtacag aagcgttaga tttagaaaca gtgccagaag aattagtaat aattggtggt
    21901 ggagtgattg gttgtgaatt tgcagagata ttcaattcaa gaggttctaa agtaactata
    21961 gtagaaatgg aagatagagt aatcccaaga atggataaag agttaagcga atctttaaaa
    22021 tattctttta gtaaaaaagg tataaatgta ttaactaaaa agaaagtttc tgaatttaaa
    22081 gaagaaggaa ataacatatt agtgtgcatt gaaggtgaag agccaataaa agcagattta
    22141 tgtctatatg ctataggaag agaagctaat ctttctggca tagaggactt agatattaaa
    22201 atagataaag gttcaatagt tgtaaatagt aaaatggaaa caagtatacc aagcatatat
    22261 gcagtaggtg atgttactgg aggtgtaatg ttagctcatg ctgcttttaa aatgggagag
    22321 gtagcagctt caaatgctct aggtatgaat aaagaagttg acttaggtgc attacctagt
    22381 tgtgtttata caattccaga agtagcatca gttggtatta cagaagagga tgctagaaaa
    22441 aaatataatg taaaagttgg taagtttaat tttgctggaa atggaagagc tttagcttct
    22501 ggccaagaac aaggatatgt gaaagtagta gcagatgcta aatatggaga aattttaggt
    22561 atacatatgt ttggatgtgg tgtagcagaa cttataaatc atgcagcagc atttaaagca
    22621 ttagagatac ctactgatga agcaagtgag ttgatatttg gtcatccatg tacctcagaa
    22681 gctttaatgg aagctttggc agatgtaaat ggagaatgtt tacacttacc taaaaaataa
    22741 atctaaacat aaattgttaa tttaagttta ttattaatat taatttatat cataaagttt
    22801 taaaacactt acttagccat accccttatt atgcaaataa tttatttgca taataagggg
    22861 tattttttat ggacaaatat agaatataga atccttgtag tttttgttgt acaaaaaaga
    22921 tagtaatata aaataacatt atcattttag aatgatttca attgtaatta ggaaaagtga
    22981 tttttttgtt tttaaaaaaa gtattatttg taaaatataa aaaaaaattt cttaatattt
    23041 gaataaaaga atagcttgat ttaaatgtta ttactttata aaaaaataat aagtacaaaa
    23101 aaaacaatat atggcatata agttgctata taaataataa ggagggaaca attataaatg
    23161 aggcaaagaa taaaaaactt aattgaaaat gaagataaga aaaatccttt aacagatgaa
    23221 gcgatttcaa gtaggttaaa tattcgaaga gaagatgtta cctttttgag aaatgagtta
    23281 aaaatagaag attcaagaca aagaagaaaa gttgttttaa ttaaagctat aaaagatatt
    23341 ttaaaagaag aaaaaactat aaataaaaat gatataacaa gaagattaaa cagcattggg
    23401 tttacagtat caagatttac agtcatacaa tacttaaaag agattcaaga ttctggtgac
    23461 ctaaaacttg ataaagagag tgatgataaa aaagaagaaa taatcaataa tagagaaaat
    23521 acagatatag catttgataa actcataggt agtagtggta gcttaagtac aattataaaa
    23581 ctagccaaag cagcaattct ttatccacct catggtcttc atactattat tatgggacca
    23641 actggtgttg gaaagagtga gttagcagag tgtatgtata aatttgcagt agaatcatcc
    23701 agattcccta agaatgcacc atttatagtt tttaatgcag cagattattc agaaaatcca
    23761 cagcttttgt tatcacagtt atttggacat gttaaaggtg cttttacagg agctgatgag
    23821 aacaaagaag gactagtatc aaaggcggat ggaggaatat tgtttataga tgaaatacat
    23881 agattacctc atgaaggtca agaaatacta tttcaactga ttgataaagg tatggtcaga
    23941 aagttaggag aaacaagact aacacataaa atagatgtaa tgattatttc agcaacttct
    24001 gagccaatag attctcattt acttaataca tttaaaagac gtatacctgt aacaattgaa
    24061 ataccagaat tagttgcaag accattaaat gagaggtttg acattataaa taatttcttc
    24121 ttaatagaag ctcaaagaat gggagcaaac atacatataa agtcagatgt attaaaagct
    24181 cttatgcttt acgattgtat aggtaatgta ggtcaactta gaagtgatat tcaagtagct
    24241 tgtgctaggg gactattaaa ccaacttaca aacaaacaaa aagaagtaaa tataagcata
    24301 tcagatgtac ctggatatgt aaaacaaggt cttataaaaa tcagaaattg tagaggtaaa
    24361 atagaaaatt atgtagatgg ggatttaatt atagattcaa atcttcaagg tgagatagaa
    24421 aagaaaaatg aagatatata tacttttcca gatgaaatat acaaacttac tgaaagaaga
    24481 catattgatt tattggaaca aggtttagat tttgatgtta taaatagaat cattggagga
    24541 gaattagaaa gttgtattca aaaatacata aaacaagtta aaaaagtttc tattagtaac
    24601 aatcttccag atataagtcc gattgttgga aaatatatcg tagagctaac ttatgaagta
    24661 atcaaaatag catcacaata cctcagttca ctagagccga atctacattt atgtttagca
    24721 gttcacctaa aagcagcttt tgagagatta aaagaaggaa aagtaatatc aaatgctcat
    24781 ttgaaagaaa taataggaaa ttatccgcta gaatattctg tagcagaaaa agtagctcaa
    24841 tatttaaaag aaagatataa cataaactta ccaaaagaag aaataggtat aatagcaata
    24901 tattttaaaa tgagtagtag aagagatatg tctgaacaaa aaaagatagg tgtacttctt
    24961 atggctcacg gaagagtagc atcttctgta tgtgaggtta caaataaact attaggaatt
    25021 aatcacgcaa ggtatttaga tatgccattt gagaagaaac cggaagaaat gattgaagaa
    25081 gctactacaa taataaaaga tattgatgaa ggtagaggtg tgattatatt agtagatatg
    25141 ggttcacttt catattttgg agatattatt tctggaaaga ctggaattaa tataaggaca
    25201 atatctagat ttgatacatt gctttctata gaagttatac gaagagcagt tttaccagat
    25261 ttaacaatag acgagatagt tgatgaagtt aaaggtatag agccacaagt aaaagaaaag
    25321 gttagtaaat ataacaatac aaaagtagat tttagaaaac ctataatttt aacattgtgt
    25381 attactggat tagggggagc ttgtaagata aaaaaaatcc tagaggaaaa tattaaaagt
    25441 ataactgaaa aggttgatat agaacctata ggtatgattg aatatccaag tcttgattta
    25501 gagataaaac aaataagcaa tgataaaaat gtaatcgcca tagttggaac aattgaccct
    25561 cagatagcaa atatcccttt ttttagtcta gtagacataa atgataaagc aaagttaaat
    25621 caaataaata acctgattga tacaaatcaa attggtaaaa caaataaaaa atatgaatta
    25681 tcagatttaa tacataaaga agatatattt gtggatttac aggttaaatc aaaagaagaa
    25741 gttctaaagt atatgagtaa aaaacttaga gagaaaggtt atgtgacaaa aaatttctat
    25801 aaaaaagtag tggaaagaga taacttattt ccaactgaat tatcaggtga aatagcaata
    25861 cctcacacag atggaataga tgtaattagg ccagctataa gtatagccat cttaaagaat
    25921 cctattgtgt ggagtaaaag taaagtaaaa ataatacttt tattagctgt agaccaaaag
    25981 tgttttaatc cattaagcac tctactttca tttatagaaa cagatgaatt taagaagata
    26041 aaaaacatta aagatagaga ttttattagg gagatgatat tagatggagt caatgaaaat
    26101 aattagtagt aatttaattt ttaaaaatat tgaagtgtct aacaatgaag atgcattaaa
    26161 gtttttggga caacgtttgt ttgacgaaca gtatgtaaaa gaaagctaca tacaagctgt
    26221 tgtagcaaga gaaaaaaagt atgctacagg attaccaaca gaaatatacg gagtggcaat
    26281 accacataca gatatagtac atgtaaatga gccagggata gcaattggaa tactgaataa
    26341 acctgtaaaa tttataatga tgggtacaga cgatactgaa attgatgtaa aagttgtatt
    26401 tatgttagca gtgaaagagc cacaagaaca attgcaacta ttagaaaaac ttatgactat
    26461 atttcaagat aagaatatac taaataatat tgttgactta agtgaagaaa gtgtgagtga
    26521 tttattaaac tctaaattga aaaattaaac atgtaaagta tgttaaaaaa tataaaaaga
    26581 ttattaaaga tttctaacta taggtatctt taaaataaaa taattttaat gggggtgact
    26641 aatgtgacta aaaagatatt agttgcatgt ggaacaggag tatgtacttc aacaattgct
    26701 ataaacaaat taaagaaagc atttcaagac attggtaaat tggatatggt taatattaca
    26761 caatgtaaag tagtagaagt tgcatcaaaa gcacctgatt atgatttaat agtttgtact
    26821 acacaagtat ctagtagtat aaaaacacca gtgataaatg gattaccttt tttaactggt
    26881 gtcggaatgg ataaattaat caatgatgta ttagaagagt tagaattata attaaaaaaa
    26941 ttaaggaaca tggtctctaa aattataagt tttagagact taaaattaaa taatggaggg
    27001 tctttatatg gagatattaa atttcattgt tggtctaggc gcacaagtaa tgatgccaat
    27061 aataatatgt atttttggtt tgatattagg tacaaaacta ggaaaatctt taagagctgg
    27121 tttaactgtg ggagttgggt ttataggtct taatactatt atagctttgc taacagataa
    27181 tttaggtcca gctacacaac aaatggttaa gaatttaggg ttaagtttat caataataga
    27241 tgtaggatgg ccagcagcat cagccatagc ttttgcatca acagtagggg ctttaattat
    27301 tccaataggt cttgtagtaa atatagttat gctaattact aataccactc aaactgttga
    27361 tgtagatata tgggattact ggcattttgc ttttacagga gctttagtgg caggggctac
    27421 tcaaagtgta atgtggggag tatttgcagc agtagcaaat atggtaattg tattggtaat
    27481 ggcagattta actgctccag gtatagaaga atatttaggt atgccaggta tatctcttcc
    27541 tcatggattt acacaggcat ttgttccaat tgctatagta gtaaataaat tattagacct
    27601 aataccaggt ataaataaga ttgagataaa tgctgataca ttgcagaaaa aatttggatt
    27661 atttggagaa cctttaataa tggggtctgt aataggtgta ataattggta ttgccgcgaa
    27721 atatgatata aaaggaatat tacaattagg tgtaactatg ggagctgtat taatattaat
    27781 tccaaagatg gcagcattat taatggaagg attattacca gtatcagaag ctgcacagga
    27841 atttattgag aagagattta aaaacagagg taagatttat ataggcttag attcagcagt
    27901 aggaataggt catcctgtta cattatcagt tgcactagtg ttggttcctt tgactatact
    27961 tatagcagct atattgccag gaaataaagt tttaccattt gcagatttag cagtaattcc
    28021 atttgcactt gtattaattg taccaattac aaagggaaat gtgtttagaa cattaataat
    28081 aggaataatt ataattactt ctggattatt aatcgcaact aacttagctc ctttgtttac
    28141 ccaaatggct ttaaatgcta gttttaaaat gcctgaagga gcaactatga tttcaagtat
    28201 ttgtgatggt gcgaatccat taagttgggt atttgtaaaa gttatgaact ataaagttat
    28261 tggaggagta gtttttggag ttatagcttt aggaatggct atttacaata ggaatagaat
    28321 aataaatgaa aataaaaagc tatctataga agaataaggt taaaacaatt ttattaagga
    28381 gtgtataggt tatgaaaaaa tataaatatg attctaaaag gtacttattc agtgttacaa
    28441 taccaggatt ttttatgatt ttaatattga tttatgcact gtttaataac tatataaatt
    28501 ttaatttgaa tatatatagt ttgttaataa ttgtctgtac ttatggtata ttcaatactt
    28561 ttatatcatc aagtaatcct aaaaaaatta ttattgatag caaatgtata cattttacct
    28621 catttatgac tacacataag tatgaaatta aagatataga aaagataaga ataagagaat
    28681 tttataataa aaaaatttat ctgaaaatta atgatggaaa cctttttaaa ggtaggtatt
    28741 ggataagaac aaatatgttt aataatagta tagcattata tagctatttc attgagttgg
    28801 aagagtgctt atatccagat tcactaaaat ttagaaataa aaaatttgag aataagaaca
    28861 tataaaaata aaaaattgaa ttgaagccta gcttaatctt ttttgataag atgtaagttt
    28921 gagttttata ctaaattttt agaattttca atcaagacaa taacacaata tctaagcaat
    28981 tagaacacaa ttaagattta taaggcatgt atatgatgaa tgggaggatt taattaatgt
    29041 tattagacaa attaagaaaa gaagtattac aagcatcttt agacctttta aactataatt
    29101 tagtgacatt aacaggagga aatgtaagtg ggagagatga acaaactggc tatatagcaa
    29161 tcaccccaag tggaatggat tatagaaatc ttacaccaag tgatattgtt atagtagatg
    29221 tagatggaaa tattatagat ggaaaatgga aagcatcagt ggatttatca gaccatttgt
    29281 atatatataa gcatagagaa gacataaata gtataattca tacacattct acatactcaa
    29341 gttgttttgc tattttaaat gaacctatag aatgtgcaag tacaactctt gcaaatgaag
    29401 taggaggttc agtaccagtt gcaaaatttt caccaccaac atctaataag atgggaaaat
    29461 gtgttattga agcaattggt gacaaaagag catgtctttt agcaaatcat ggcgtaattg
    29521 cagttggtcc atctgtagga catgctctta cagccgctgt aatgttagaa gattcagcaa
    29581 aagtatatta cttagcaaaa tcaattggca caccagtgct actgcctgat gaagaaattc
    29641 aaagagcgag agatgtattt tttaatgttt atggtcagga taaataaatt agataaagtt
    29701 ttaaaagtaa tatgaaaata tagatattca atgatattaa ggagatttat aatgatataa
    29761 taagtttaat agttaagaag taaaatatga aatgtaatag tacataaatt catttaagac
    29821 taaaaaataa taagataaaa taaaaaagga gtctgtattt ctatgtatta cttattaaac
    29881 aaaagcacaa atccttactt taatttagca ttagaagaat atctattttt aaatgataaa
    29941 tataatgatg atattataat catatggaga aatgaagaat ccatttttat aggtaaaaat
    30001 caaaatccat atcaggaagt acatcatgat gttattgaaa agggagaaat tcctatttta
    30061 agaagaatat ctgggggagg cactgtttat catgacttag gtaatatcaa tatgtcgttc
    30121 atacaaaaag ataggcaact tcatgagata gattttttgg agcatactaa gtttatgcaa
    30181 aagatgcttt caaccttagg actagatgta tctataactg aaagaaaaga tttattttta
    30241 aatgggaaaa aaatttcagg ttctgcacag tctataaaaa gaaaaaattc tctttatcat
    30301 ggaactcttc tttacgattc agatttaaat aaattaacta aatatttaaa ttcaaataaa
    30361 gcaactgaat caaatgcaac taaatctgtt tcaagtaaag ttacaaatat taagttttta
    30421 ctcgaaaaag atataaatta ctttttagat tattgtgttg aatatttaaa atctaatata
    30481 aagaatataa aagaacttga attggatgaa gaagatatta aaagtgtata caatatagga
    30541 gaagataaat ataaaaagat agagtggaca tatggtaaaa cgccgaaatt tcaagtagtg
    30601 ctaccattta ataatgaaag taaaataaat atacatgtta acagatggaa agtatcaaaa
    30661 ttttttattt cttatgatga taatttaatt aatatggaca agttgttaga tttgaatttc
    30721 tttaaagaag aaataaaaga gagtatttta gaaaattatc cacattacat agatttaatc
    30781 aatttaatat tttaatataa aacctctaat ttgataaaat tgtcttatat caaactagag
    30841 gtttttaata ttcttgcata ttaacaacca ttacaaaatt gtaaaattaa aaacaacact
    30901 attgaaaaat caatatatat tgtaataaat atacgtgttt ttcattagca aatagacatt
    30961 aactatttat acaactgaaa atttaatata taattaatca gatggttaaa ataaaaatat
    31021 taaaagtttt aataaatgat ggttaaaaat aaaatacgag gtgataccat gtatagtgtt
    31081 ataattgttg cggcgggaag cggaaggaga atgaacttag atataaacaa acagttcata
    31141 aaattaagag aaaaagaaat tattgctcat acaattcaag ttttttatga aaatataaat
    31201 atagatgaaa tagtggtatg tataaaaaaa gaagaagagg atttttttaa tgaaaatata
    31261 ataaataagt ataactttaa aaatataaaa atagcatatg gcggaaaaga aagacaggac
    31321 tcaatatata atggtttaaa aaaattagat aaaaattgtg atatagtact tatccatgat
    31381 ggagcaagac catttgtcga ccatagaata atcaatgaat ctataaaggt agctaaagaa
    31441 aaaaaggctg tggttgtagg tgttccagta agtgatacta taaagatagt gtcagatgga
    31501 acagtacaag aaactccaga aagaaatttg ttgtgggcag cacaaacccc tcaaaccttt
    31561 gaatataatc tcataataga tgcttatgaa caagcatata agaacaatta ttatggtact
    31621 gatgattcaa tgttggtaga aaatataggt caaagtgtca ctatggtaat gggttcttat
    31681 gaaaacataa aaataactag tccagaagat ttaaatatag cagaacaaat attaaatatg
    31741 gaaaaaagag atgaagttaa cagtagaagg agaattatat gagaatagga ttaggatatg
    31801 atgtacacaa attaacagag gatagaaaac ttataatcgg tggtgtagaa ataccacacg
    31861 ataaaggttt gttgggtcat tctgatgctg atgtattgat acatgcaata atggattcta
    31921 ttttaggtgc tctagcatta ggagatatag ggaagcattt cccagataca gatgaagaat
    31981 ataagggagc agatagtatg aaattattag aacatgtgta taatctaata acaagtaaag
    32041 gatataaaat tggtaatata gatagtacta taattgctca aagccctaag atggctccat
    32101 atattgagag tatgagaagt aatatatcaa aagttttaaa cacagacatt gataatataa
    32161 acataaaggc aactacagaa gaaggtcttg gttttacagg agcaaaacag ggaatagctt
    32221 cacaaagtat ttgtttatta ttgttgacaa gtcagaataa ttaatatatt attagtaata
    32281 agtttaatgg atatcaaata ataggcctat atagaatatc tataatgtgt gaatttaagt
    32341 gaaattaata taaatataaa attagctatg ataaagaaat agtaaaatga tgaaatcttc
    32401 acagagagaa aacgttggtg agagttttca agaggaagtc tttgaacctg tcttttagct
    32461 taaaggtata aaaatcttta cggtaggaac cgttatattc ccaaaagaga gctatttata
    32521 tagctaacta gagtggtacc gcggaaatta aacctttcgt ctctatactt gtatagagat
    32581 gagaggtttt ttatattttc aggaagttaa tattcaggag gaatctatta atgaaaatgt
    32641 caaaaatgtt tatgccgaca ttaaaagaaa tcccagcaga tgctgagatt acaagccatc
    32701 aattaatggt caggtcaggc atgataaaaa aaatgacttc aggtgtttat aaccaattac
    32761 caatggggtt aagagtgttc aaaaaaattg aacaaataat tagagaagaa ttaaataaaa
    32821 aagattgtca agaaatactt tgtgcagcct tgcttccttc tgaattatgg aaagagtctg
    32881 gaaggtggac tgctatgggt gaagaaatgt ttagactaaa agacagaaca gaaagagagt
    32941 actgtcttgg gccaacacat gaagaagcat tcactgatat aataagacaa gaaattactt
    33001 catataaaca attgccactg aacttgtacc aaatacaagt aaaatatagg gacgagagaa
    33061 gaccaagatt tggtgttatg agaactaaga cttttacaat gaaggatgcc tatagttttg
    33121 atgcagatga taaaggttta gataagtcat atcaagatat gtttgatgca tatgtaagta
    33181 tatttgatag atgtggattg gagaatagcc ctgtacaggc agattcagga gctataggag
    33241 gttctacatc agccgaattt atggttaagt cagaagttgg tgaagatgaa gttgtatttt
    33301 gtagtggatg tgattatgca gccaatgtag aaagagcaga atcttgtaat ctagcatcac
    33361 aaaaagaaga aatgaaagaa ttagaagagg ttcatacacc aggagcagct acaataaaag
    33421 agttagaaga atttttaaaa acttctccag acaagtttgc taaaacttta gtatatgagg
    33481 cagatggaaa aacagttgta gttgttgtaa gaggagatag agaagttaat gaaatcaaag
    33541 tatctaatgc tataggttct gttatagaat ttgctcttgc tacagatgat gtagtgagaa
    33601 aagtaactaa tgcagaagta ggatttgcag gtccaatagg tataaatgct gactatgtat
    33661 ttatagataa agaaattgtt gaacaaagaa acatagttgt tggggctaat aaaactgagt
    33721 accatataaa aaatgctaac tatggaagag attttgaagg aatagttgga gattttagaa
    33781 atgttcaaga aggcgacaag tgtatagtat gtggtaagcc acttgaaata gctagaggtg
    33841 ttgaagtagg acatatattt aaactaggaa ctaagtactc agaatctatg aatgcaaact
    33901 tcatagacaa agatggaaaa tcaaagccta tagtaatggg atgttatgga ataggtgttg
    33961 aaagaacagc agcagctata attgaacaac acaatgatga gaaaggtata atatggcctt
    34021 tgtcagtagc tccataccat gttgtgataa ttccagcaaa tatgaaaaat gaagaacaaa
    34081 tatcaatagc tgaaaatata tataatgatt tgcaagctat gggagtagaa gttttattag
    34141 atgacagaga tgaaagaata ggtgtcaaat tcaatgattc agaattaata ggaataccta
    34201 tgaggattac agtaggtaaa aatattaatg aaggtaaagt agaatttaaa cttagacata
    34261 aagaagataa agagataatt gacatagaag aaataaatga aaaagtaaag gcagagttta
    34321 tcaggaataa tgttaggtta ggacaataac ttaaataaga tattgtatag ataatcagag
    34381 ttgtacttta tagacaccat tttatctata tgaaggatat aaaatggtgt ttttttataa
    34441 aacaaataaa tgaggaggta attgaaatgg caaaaaatga aaaacaattt gttgaagaaa
    34501 taacaaagat ggaagatgat tttccacaat ggtatacaga tgtaataact aagactgact
    34561 tagttgatta tgcaccagta aaaggtttta tggttgtaaa gccatatgga tatgcattat
    34621 gggaaaagat gcaagaattt atggataaaa aatttaaaga aactggacat aaaaactgtt
    34681 attttccatt attaatacca gagagtttat taaacaaaga agcagaacac gttgaaggtt
    34741 ttgctccaga agttgcatgg gtaacacatg ggggaaataa aaaattagaa gaaagattat
    34801 gtgtaagacc tacttcagag actataatat gtacaatgta tgcgaaatgg ttaaaatcat
    34861 atagagaatt accatatctt tataatcaat ggtgctcagt tgttagatgg gaaaaatcaa
    34921 ctagaccttt cttaagaaca tctgagtttt tatggcaaga aggtcatact ttacatgaaa
    34981 cagcagaaga agcacaagaa gaaacaatac aacaattaga agtatataaa gcgctatgtg
    35041 aagaattact tgcaatgcca gttgtagctg gtcaaaaaag tgaaagtgag aaatttgcag
    35101 gtggtgaaag aacatatact atagaagcta tgatgcatga tggtaaagca ttacaatcag
    35161 gtacaagtca tttcctaggt caacacttta ctaaggcttt tgatattact tttgcagata
    35221 gagaaggaaa cttagctaat ccataccata catcttgggg agcatctaca agacttatag
    35281 gtggacttat aatgactcat agtgataaca gaggtttagt attaccacca agagtagctc
    35341 caattcaagt tgttatagtt cctatagctg ctaaaaaagg aaatgtaatg gagactgttg
    35401 ataaaatata tgctgattta aaaactaagg gagtagccgt agaagttgat gatagagata
    35461 actatacaac tggatggaaa tttaatgagt gggaaatgaa aggtgttcca gtaagagttg
    35521 aaattggacc aaaagatata gaaaataatc aagctatggt atttagaaga gatactttag
    35581 aaaaggattc tatgccttta gaaggattag ctgatgctat atgtgattta tttgatgtta
    35641 tacacaatga tatgtttgaa aaagcaagaa aacatagaga agacaataca tctatagttg
    35701 aaaatatgga tgagtttaga aaagctttag aagaaaagcc aggattcata aagactatgt
    35761 ggtgtggaga tgcagaatgt gaagctaaga ttaaggaaga aacaggagca actataagat
    35821 gcttaccttt tgaacaagaa aacttaggtc acaagtgtgt gtactgtgga aaagaagcag
    35881 atagtatggt agtaatggca aaagcttact aaaatacatt taaataaaaa ctggggtaga
    35941 aagtatttaa tactttatcc cagttttttt attttttatg agcaataaaa ttgtatttgt
    36001 aactatgtca taagataagt catagatact tatattaaaa agaatataat agagaactat
    36061 ttttaataat tacatattaa tgaaagcaaa atatgtttgt tatatagatt ttcaataaag
    36121 caaacaataa atataaatga atattaataa agagtacttt taatttttag aaaataaatg
    36181 acttaaaaga aagatttatg aaattaaaac taggattttc tgatataata aacatgtgta
    36241 tttttaaata aaaaggatat tattattaga tttgattaaa aataaacttg caaaacaatg
    36301 tactagttta atattatagt tatattgaat aaaatatcag gaggaagtaa aaatgaatgt
    36361 aagagtaagg tttgcaccaa gtccaacagg atttgtacat ataggtagtt tgagaactgc
    36421 tttatataac tatttatttg caaaaaaaat gggcggagaa tatatattaa gagtggaaga
    36481 tacagaccaa agtagattag tagaaggtgc tatagagaat atgcttaatg ctatgaaatg
    36541 ggctggagtc aaccatagtg aaggtgttat attagatgat agtggaaaag tagtacaaaa
    36601 aggagaatat ggtccatata ttcaatctca aagattagat atataccaag aatatataaa
    36661 gcaactttta gatagtggaa aagcttatta ctgtttctgt acaaaagaaa gactggatga
    36721 agtaagagat gctcaaagag cagcaggaga aacagctaaa tatgatggtc attgtaagaa
    36781 tctttctaaa gaagaagtag aagcaaatat aaaagcagga ataccatatg ttataagatt
    36841 aagattgcca gaaaatcata caattaagtt tacagactta gttagaggag atatggaatt
    36901 taatactaat gatttagatg accaagtatt aatgaaaaca gatggatttc caacttatca
    36961 ttttgcagta gttgttgatg actatttgat gaaaattaca catgttataa gaggagaaga
    37021 atgggtatca tctactccaa aacatgttta tttatatgaa gcttttggat gggaagcacc
    37081 tgtatttgtg catttaccaa atatacttaa taaagaaaag aaaaaattaa gtaaaagaca
    37141 aggtgatgta gctgttgaag actttaagaa aaaaggatat ttaccagagg gattagtaaa
    37201 ctatgtagca ttagttggtt ggtctccaga agataatcaa gaattgttta caatgaagga
    37261 attagaaaaa gccttctctg tagaaagagt atctaaaagt ggcggagtat ttgatactga
    37321 aaaattaaat tgggttaatc agcactatat aaaagatggt gatgatgctt atctaacaga
    37381 cttagcaata ccatttttaa tagaagatgg atttataaca gaagaagaag ctactagcaa
    37441 atatgatttc ttaaagagta tgatatcagt acttaaagag aaattacaat atgtaaaaga
    37501 agttactgaa catgcaagta tattttttgg agataaaata gaggtagaaa ctgaagaggg
    37561 aagtgagttc ttaagattag aacatatacc tactttaata gatgctttaa gagaaaaaat
    37621 agaaaaagca gaggtattaa atgctgagtt cgttcaagct atgcttaaag aaatacaaaa
    37681 agagtacaaa ataaaaggga aaaatttatt tatgggctca agaataattt taacagggca
    37741 aatgcatggc ccagatttac caaaagtaat ggaagtttta ggaaaagaaa cttgcttaaa
    37801 tagaatagct tatgtaaaaa ataatatatt atagtcataa aagtccgcta atttagaagc
    37861 ggactttttt tgtgttagta tttggtgaaa atatctgctt aagaaaatga aggtatttaa
    37921 ggtaataaat ttaatatata aaaagcaaag caaaaatata aataaaatat gaataataaa
    37981 ctaataaaat attaagcata gaagaaggga atctattatt ttagtttgat ttaattatta
    38041 agggggtaat tactatgtat agtaatattg atgatgttaa aaaggaatta aaggagctat
    38101 gcttagaata tgttacaatt ttagaaaaat taaaggatga aaaaatgatt acagaagaaa
    38161 cttttgaaaa gtgctcttct caaaaaaaga tatttttaga agaacaatag aggatggtaa
    38221 tagtattatt attatgtagt ttgaaagata gtacgaaaaa tgatagtaat actattattt
    38281 attgcaattt tcaactataa tataatatat aatgttaaga aaataattta taaaaataca
    38341 gtgaggtgat gagtgtgaaa gtttataata cattgactag agcaaaagaa gaatttgttc
    38401 cattagaaga aggaaaggta aaaatgtatg tgtgtggtcc aacagtttat aactatatac
    38461 acataggtaa tgcaagacca tttataatat ttgatactct aagaaggtat ttagagtata
    38521 gaggatatga tgtaacttat gtacaaaact ttacagatgt tgatgataag attataaata
    38581 gaagtcatga ggagggaata tctcctgaag aagttgcggc taagtatata aaagaatatt
    38641 ttgtagactg tgatgggcta ggaataaaaa gagctactgt tcatcctcag gttacagata
    38701 atatacaaca gattatagag tttataaaag agttggaaga taagggatat gcatatgcag
    38761 taaatggtga tgtatatttt gatacaaata agtttgaagg atatggaaaa ctttcaggtc
    38821 aaaaacaaga agatttagaa gcaggggcta gaattgaggt taatgaccaa aaaagacatc
    38881 ctatggactt tgttctttgg aaggcaaaaa aagaaggtga gccaggatgg gatagccctt
    38941 ggggagaagg tagaccagga tggcatatag aatgttctgt tatgtctaag agatatttag
    39001 gtgaaacaat tgatatacat gcaggtgggc aagatttaac attcccacat catgaaaatg
    39061 aaatagctca aagtgaagct agaagtggaa aaacattctc aaaatactgg atgcataatg
    39121 gatatataaa tataaatgat gaaaagatga gtaaatcaaa aggtaatttc tttacagtaa
    39181 gagatatatc aaaattatat gatttagaga tagtaagatt tttcatgtta tcagcacatt
    39241 atagaaatcc agtaaacttt agtgatgaga tgttaaatca agctaaagca ggtcttgaaa
    39301 gattgtataa tactaaagaa aaattagaat ttacactaag taatttagta gaatcacctt
    39361 tgacagaaaa agaaatagaa cttgttaaag aattagataa ctttagacaa aagttcatag
    39421 atgctatgga tgatgatgtt aatacagcag acgcagtaag tgtaatattt gaattagcta
    39481 agttaataaa ttctaatgta gatgaaaatt cttctttgga gtttgctaaa aaatgcttag
    39541 atgaatttaa tgaacttaca ggagtgttaa atattgtaaa taagaaaaag gatactgtat
    39601 tagataagga tattgaagaa ttgatacaaa aaagaactga tgcaaagaaa aataaagagt
    39661 ttcaattagc tgatgatatt agacaacaat tactagatat gggcattgta ttagaagaca
    39721 caagacaagg tgttaagtgg aaaaggatat aaaaatggaa aaaaccgaat tagttactat
    39781 gtcaccatta gttttagcat acttaggtga tacagtgtat gagacatata taagagaata
    39841 tttaattaga cagaatacac aaaaaaaagt taatgatttg cataaattag ctataaaata
    39901 tgtaaaagct aaagctcagg caactataat tcatgaaatt gaaattgagt taactgaaga
    39961 agagagtaaa atttataaaa ggggaagaaa tcaaaagtca aatacttctc ctaagaatgc
    40021 agatattata gactacaaac atgcaactgg gtttgaggca ttagtgggat atttgtattt
    40081 aaataatgaa atagagagac ttcaatatat tatcaataaa ggaatcaaaa ttattgaaag
    40141 agatatgtaa atattaaaga gtgttgtctt aataataagt aataaggcaa cacttttttt
    40201 ataactaatg tataataaat atatagaaat tagtaatgag aaaggataaa aaattatgaa
    40261 agtaaaatta atatcacata cacctgaacc agaaaaagta atagctatgg cagctaagct
    40321 atgttattca ccagttggga cagacgaaat agaaaaagac ttaacagatg aaagtataga
    40381 gaaattttta aatatgttat taagtatagg tcatgggtca atattagaac atgcttcatt
    40441 tacattttca atagaaggta tttcaagggc ttgttcacat caaattgtta gacatcgtat
    40501 agctagtttt tcacaacaaa gtcaaagata tgttaaatta gaacaatttg agtacataat
    40561 tcctccagaa atagaaaaaa tagaaaaggc aaaagagtta tttatagact ccatgaaaaa
    40621 agaccaagag aattatgata agttagttga aatattgttt gaaaatcatt ataatgacct
    40681 aataaaaaac ggtaaaaatg agaaaacagc taaaagacaa gctgaaaaga aagcaataga
    40741 agatgctaga tacgtatttc caaatgcatg tgaaacaaaa atggtattta ctataaatgc
    40801 tagaagtttg tttaatttct ttgaacatag atgttgtgaa agagcacagt gggaaataag
    40861 aaatttagct gtggagatgt taagagaggt taaaaaggtt gctcctatat tatttaaaaa
    40921 gactggtcca agttgtgtaa atggaagttg tccagaagga actatgactt gtggagatat
    40981 agttcaagtt agagaaaaat ttaaggcctt gtaggaggtg aataatttgg cgagtataga
    41041 aggaagaaat ccggtaatag aagcaataaa aagtgacaga gaaatagata aaatattaat
    41101 tgcaaattca gctaaagaag gttcaattaa gaaaataata ggaatggcta aagataagaa
    41161 tataattatt cagtatgttg ataaacataa gctagatgaa gtaagtacaa gtcattcaca
    41221 tcaaggtgta atagcttatg caagtgagta taagtattat gaattagatg agctaataga
    41281 tttagcaaag aataaagatg aagacccatt tttcattata ttagatgaga taacagaccc
    41341 acataatttg ggttcaataa taaggacagc agatgcagta ggagcgcatg gagttatcat
    41401 tccaaaaaga aggtctgtac acataactcc tgtagttgcc aaagcatctg ctggagcagt
    41461 agaatatatg ccagtttgca aggtaaccaa tatagttaat acaataaaaa gactcaaaga
    41521 agaaggattg tggattgctg cagctgatat ggatggagaa actttttata aacaaaatct
    41581 tactggtcct cttggtgtag taattggaag tgaaggtttt ggaatatcta gattggttaa
    41641 acaaaactgt gactttatag taaaaatgcc aatgataggt aatgttacat ctttaaatgc
    41701 atctgtagca ggtggaatac ttttatatga gatattcaga cagagactgg ataaaagtaa
    41761 ataggcatga aaaaaaatat aaatcattac ttaataatag atggctacaa tattataaat
    41821 gcgtgggaca atttgaaaga actggctaaa gaagacttag aagattctcg agaaaagctt
    41881 attgatgaca ttatagaatt ttctgagttt atggggtata agactataat agtttttgat
    41941 gcttataatg ttaaaaattc tcgagaaaaa gttgaaaaaa gaaaacacat aacaatagtt
    42001 tatacaagag aacaccaaac agcagatagt tatatagaga aatttataac ttctttatca
    42061 aagtatgatt atgtaaaagt agccactaat gactatgcag aacagcaaat gattttagga
    42121 aaaggtgcta ctagaatgtc tgcaagagaa ttaaagttag agttagatcg ttccaagaat
    42181 aaaatgaagg aaaaaaatat cggtttgagg aaaaaaattc agcgaaattg gttggaggaa
    42241 aggttagatg aagaaacatt gtcgaaactt gagaacattc gtagaaagcg ttgaaattac
    42301 taggttcttt agactataat atacctataa atttgtttta tatatggggg agatttacat
    42361 gttggtagca aaagaaaaaa gttatgagtt agtggacaat tgtcagcagg atgagtataa
    42421 cattgtatta agagcaagtg aaggggataa aatagcacta gagtacatta ttactaaata
    42481 cagaaatttt gtaaaggcaa aagcaaagtc atatttttta attggagcag ataaagaaga
    42541 tataatacaa gaaggaatga taggccttta taaggcagtt agagattttg atggaagtaa
    42601 gaccaattca tttaaatgtt ttgcggagat atgtataaca agacaaataa taactgctat
    42661 aaaaactgca acaagacaaa aacatatacc tttaaattca tatgtttctt tgaataaacc
    42721 aatatatgat gaagagtcag acagaacact tttagatatt atagctacaa gtatagtaac
    42781 agatccagaa gagcttataa ttagtaaaga agagttaaaa aatatagaat caaaaatgaa
    42841 cgaattgtta agtgacttgg aattagaagt actagaatta tatttaaatg ggaagtcata
    42901 ccagtttata gcggacaagc ttaaaagaga tgtaaagtca atagataacg ctttacaaag
    42961 ggttaagaga aaattagaaa aacatcttga aaacagaaat gattaatagt aaaatactta
    43021 ccataagctt attgtgagca aaattaattt atatcgaaag ataagggagg aaattcaaaa
    43081 tggctaaagc taaatacgaa agaacaaaac ctcatgttaa tatagggaca ataggacacg
    43141 tagaccacgg taaaactaca ttaacagcag caataacaaa aacattatat gacagatatc
    43201 aattaggaga agcagtagat ttcgcaaaca tagataaagc tccagaagaa agagaaagag
    43261 gaatcacaat atcaacagca cacgttgagt atgaaacacc aaatagacac tatgcacacg
    43321 ttgactgccc aggacatgct gactacgtta agaacatgat aacaggagca gcacaaatgg
    43381 acggagcaat attagtttgt tcagcaacag atggaccaat gccacaaaca agagagcata
    43441 tactattatc aagacaagtt ggtgtaccat atatagtagt attcttaaac aaatgtgaca
    43501 tggtagatga tgaagaatta ttagagttag tagagatgga agtaagagat ttattaacag
    43561 aatatgattt cccaggagat gacactccaa tagtaagagg ttcagcatta atggcattag
    43621 aagatccaaa gagcgagtgg ggagataaga tagtagaatt attcgagcaa atagatgagt
    43681 atataccagc tccagagaga gatacagata aaccattctt aatgccagta gaggacgtat
    43741 tctcaatcac aggaagagga acagttgcaa caggaagagt ggaaagagga gtactaaaag
    43801 tacaagacga agtagaatta gtaggattaa cagaagcacc aagaaaagta gtagtaacag
    43861 gagtagagat gttcagaaaa ttattagacc aagcacaagc aggggataat ataggagcat
    43921 tattaagagg agtacaaaga aacgagatag aaagaggaca agtactagca aagactggat
    43981 cagtaaaggc acacacaaag tttacagcag aagtatatgt acttaaaaaa gaagagggtg
    44041 gaagacatac accattcttt gatggatata gaccacaatt ctatttcaga acaacagatg
    44101 taacaggagc ttgtaagtta ccagaaggaa tagagatggt aatgcctgga gataacgtaa
    44161 caatggaagt agacttaata aactcaatag ttgtagaaga gggattaaga ttctcaataa
    44221 gagaaggtgg aagaacagta gcttcaggag ttgttgctac aataat
//
LOCUS       DAEPXF010000011        41799 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain HB27
            SAMN13376389-rid12479423.denovo.012, whole genome shotgun sequence.
ACCESSION   DAEPXF010000011 DAEPXF010000000
VERSION     DAEPXF010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13376389
            Sequence Read Archive: SRR10518432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 41799)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 41799)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001069624.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001069624.1
            Genome Coverage       :: 315x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/15/2021 01:54:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,165
            CDSs (total)                      :: 4,076
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 89
            rRNAs                             :: 10, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 10 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 19 of 69
            Pseudo Genes (incomplete)         :: 44 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 10 of 69
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41799
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13376389-rid12479423.denovo.012"
                     /strain="HB27"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="China: Hebei"
                     /collection_date="2017"
     gene            complement(<1..1882)
                     /locus_tag="KRL67_000542"
     CDS             complement(<1..1882)
                     /locus_tag="KRL67_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH1521447.1"
                     /translation="MKKYEIFIFISIIVLCFISFPHKVFGQEENCLEDFERWVEENKN
                     NKEEIVYTLSCDMVIDEEFRFYIPYDSNFTIDTNKYKILIKNHGRFIIDDNELNIIGE
                     GGKEGVIHVENGGSISIGINNIIATDGTALYVEEGGDLHIKSSYESVEKIKANGKDAI
                     GIYTENDIRILNKDIEVNGERAIGVYSKGDVEIEETSIKAYSSNNENGLLKDNNKLVQ
                     SIISESKKVYIIDDYNELIPSIAEDSGYNIVKCCHRGIGAFSDEIKVSKDDKIENIKF
                     PQYMVLETSSGNQLDIDVEWDFTDYYEKLEKGENFNITGKFKTEMLNKEKIIINDDVI
                     PILNVSVIEKKPIDNLELEFQNTKNGYVAALFYDMPYSASKVFVEYSSDGINWTSEEQ
                     EDIRDQAILFFEDFKLRCFRVKVVGGLREGYSNIVFKPGFIMGGGDDQETPDDEREND
                     DIDGDRGGGGRDDPDRDEDNNNQDDNNQDDNNENENNDSNNNQNNNNENDNSDEKQPN
                     APKDDVESSTGSNENKNPNDIPKNDNKKNNSDKEKGFDNKSSITENDGNYDLNSKSVF
                     GTNEHSFPYRESISNKNQDINKNKSNVSDYDGIINNNYKLESKEYNLKKDIIFLVCIL
                     TPI"
     gene            complement(2066..3541)
                     /gene="spoIVA"
                     /locus_tag="KRL67_000543"
     CDS             complement(2066..3541)
                     /gene="spoIVA"
                     /locus_tag="KRL67_000543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage IV sporulation protein A"
                     /protein_id="HBH1521448.1"
                     /translation="MNNNIYEDISKRTQGDIYIGVVGPVRTGKSTFIRKFMEKLVIPN
                     IDNEFKKDRTRDEIPQSGSGKTIMTVEPKFVPADGVEIKIKDTVSLKVRMVDCVGYIV
                     EGALGHEEGGKQRLVSTPWSQEAMTFEKAAEIGTKKVIKDHSTIGIVVLTDGSVTGID
                     RKSYVEPEERVIQELKNLKKPFAVVLNTLSPKSEETSMLRSELEEKYEVPVLPMNVVE
                     MEEEDIEEVMEAVLYDFPLTEIRINLPKWVEGLERNHWIKSSIITTLKQSIIDIGKIR
                     DIEGIMQGFSELEFLEDTGVDNVELGEGVINIDLQTKQDLFYNVLEEKSGFKIEGDYQ
                     LLSLITRLSKVKNEYDKIESALIDAKIKGYGVVAPSLEELSLEEPEIMKQGKQYGIKL
                     KANAPSLHIIKADISTEVSPIVGNQNQGEEMIKYLMEVFEEQPADLWESNMFGKSLHD
                     LVKEQLQSKLYTMPEEIRVKMQKTLQKIVNEGSSNIITILL"
     gene            complement(3713..5008)
                     /locus_tag="KRL67_000544"
     CDS             complement(3713..5008)
                     /locus_tag="KRL67_000544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBH1521449.1"
                     /translation="MSVEVNKPSIWKNFKVLIILFVAGAFIYALPYLKNYYYDTFAQA
                     FNLTNTQMGSLGSIYGLLAMVSYLFGGFLADRISARKLLSGSLIITGISGFVLTLYPP
                     YIVVFLIHALWGITTILTFWPALVKAVRMLASENEQGKAFGFMESGRGITNAIHLSLV
                     LILFGYISSKVSDKSGLTAIIVLYSAINVIVGVLVFFKLKDNEKEVSAKFDKEAFFAV
                     IKMPHTWIITIIMFCSYSANMSFYYFTPYSTEAFGATVVFASAISILAQYCRPVASAA
                     SGLLGDKVGSSKIISYGFILMIAGLLGVIFTPPKSSMIYILLIGCVAIYVSMYAIQGL
                     HYSLLEEGNYSLSISGTAIGIVATLGYLPEVLCPLAAGKILDAFPGVTGYRYYFIVLT
                     IVAIIGLVFTFIWMNMTKERRKELIEMNSKSNENTTKVS"
     gene            complement(5192..6601)
                     /locus_tag="KRL67_000545"
     CDS             complement(5192..6601)
                     /locus_tag="KRL67_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBH1521450.1"
                     /translation="MKKKIGFWDLVFMNISALFGIRWIAKSTASSFGLGLGSIPVWLL
                     FAFIFFVPASLICAELAATYPKDGGLGEWVKQAYGEKWGFMVSWLNWTSKIFWYSSFL
                     TFLAINIAYMLGNPDLSNNKMFILALSLIIFWLLSLVSTRGMAFGKFFTNTGALGSTI
                     PAILLIVMAFMSVVILKKAPSASIYTIQNIIPKIDANSLVSISAIIFALSGAETTANF
                     ITEMDNAKKNFPKAILTVAVLIGGIYILGSVAITMILPPDEIAASTGILDALAKVAQD
                     LGIGSWFIRIVAFGITLSVLGALILYIAGPVKMLFGNVREGVFPKQLTVTNKHNIPSN
                     AVIIQAIIVSLLLVGTNLLPSVDNIYNVLVTMTALTALFPYVLLFTSYIRLRKTRPNE
                     ERPYSIAKSDSVCINIARMVLVVTVVGILFSTAPVMETLKDNIIYEIEMIGGGLFVII
                     TGLTLWKNYEKKISNNSGR"
     gene            complement(6653..7792)
                     /locus_tag="KRL67_000546"
     CDS             complement(6653..7792)
                     /locus_tag="KRL67_000546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HBH1521451.1"
                     /translation="MSNNMRLERLRKYMNEKSIEIALIFEPDNQFYISGFKAITYSRP
                     IVTVVTQDKIELIVPGLEELHAKEVAKVDNVYVYYEIPEMKEHGISHKHYLDIILNKY
                     PKGTTVGIEKDIVSASFSEYITDKSFTIKDVGSKIFEMRYVKDAKEIEFLKIAGYLSD
                     IGIKGSLENVRVGMSELEFDVAGDNALLKYVSENYPDTYIGFANWTCSGIDRTAQPHL
                     DSNTRILQRGDIVIHSRQVWYENYRAENERTFIIGKPTERQKEVFKIAVEAQQAGLDT
                     IKAGIPARMVDEAARAVVAKYGLELYSNHRIGHGLGLSEHEEPYLRFDNELILEEGMV
                     FSMEPGIYIPGVGGFRHSDTAIVGKNGATIITNYPRSVEELILDI"
     gene            8111..8761
                     /locus_tag="KRL67_000547"
     CDS             8111..8761
                     /locus_tag="KRL67_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBH1521452.1"
                     /translation="MTNKELQKKRILMYFIEAAQNIMENEGIENITLRKVADMAGYNS
                     STLYKYFKNLDHLISFASIKYFKDYNLNISRCIENVNDEYKKYIIMWKLFCKHSFDNA
                     QAFYQVFFNLSSDELSYITKQYYDMFPEDLGIHDSDISLMITGESIKERNKILLNNLV
                     DTGYIPAKDLDIINDIIISFYQNLLFLKKNTGKNSDDDELTMKMISSIEYIIKHAK"
     gene            complement(8918..9937)
                     /locus_tag="KRL67_000548"
     CDS             complement(8918..9937)
                     /locus_tag="KRL67_000548"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HBH1521453.1"
                     /translation="MEKVCVLGTGSWGSALALGLAKKGNDVSMWTRKEEQAKKINRTK
                     ENTDYLPGVLFPNNITISTDIEKTIKDCKVIVLAVPSQAVRSTCQKIKPFIKEGQVIV
                     NVAKGLEKGTGLRLSQVCEEELPQNPYVILSGPSHAEEVARDIPTTVVVASKDLKIAQ
                     MIQDLFMSPKLRVYTNPDIVGVELGGALKNIIAFGAGICDGLGYGDNAKAALMTRGIS
                     EMSRLGIAMGANMSTFAGLSGIGDLIVTCTSMHSRNRRAGILIGKGMSLEDTLKEVKM
                     VVEGITATEVAHDVAEKLDIDMPITNAIYSVIKKGSNPKEVGIELMMRSKKHEMEEVV
                     LGDDI"
     gene            complement(9968..10582)
                     /gene="plsY"
                     /locus_tag="KRL67_000549"
     CDS             complement(9968..10582)
                     /gene="plsY"
                     /locus_tag="KRL67_000549"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HBH1521454.1"
                     /translation="MEIFSYIIIAVVAYLLGNISTSYIVAKRIAGVDIRTQGSGNAGS
                     TNVLRTLGKRAGAMTFLGDVLKGVMAVLISEFAARLVGIDTLLAGYLAVICVVAGHNW
                     PAVLGFRGGKGVATSLGAMLAVNPVITLMCLAVFILVVAITKYVSLGSVVGIGCSPIF
                     MIMVKNKAGLIVALFLTASVIYNHRANIKRLLNGTERKIGQKKE"
     gene            complement(10617..11936)
                     /gene="der"
                     /locus_tag="KRL67_000550"
     CDS             complement(10617..11936)
                     /gene="der"
                     /locus_tag="KRL67_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012063975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HBH1521455.1"
                     /translation="MSRPVVAVVGRPNVGKSTIFNKFAGKRISIVENTPGVTRDRIFA
                     EVEWLDKYFTLVDTGGIEPDSEDIILSQMRNQAMLAMDMSHVILFIVDGKAGITAADK
                     EIAQLLRKTKKPVILVVNKIDSQSQFDNIYDFYELGFGTPFAVSGANSMGFGDLLDEI
                     VENFPAGLDTEYEEDIIRVAITGKPNAGKSSILNKILGEERVIVSPIAGTTRDAIDTY
                     FEKNGQKFLLIDTAGLRRKSKIYETIEKYSVIRAMSAVDRADVVLIVIDALEGVTEQD
                     TKVAGIAHDEGKGCIFVINKWDLIEKDNKTMSNYTKDIKEKFPFMMYAPIVFVSAKTN
                     QRMNKILDTVEYVSNEHSKRISTSALNEVIGEAVMLNQPPSDKGRRLKIYYGTQTDIR
                     PPKITLFINDKDLTHFSYQRYLENKIRENFGFEGTSIKFEYRQKNKK"
     gene            complement(11970..13298)
                     /locus_tag="KRL67_000551"
     CDS             complement(11970..13298)
                     /locus_tag="KRL67_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF512 domain-containing protein"
                     /protein_id="HBH1521456.1"
                     /translation="MEVNVKKVNNIISKVYKDSIAEEIGIEVGDLLISVNEQPIHDII
                     EYRFLLSDEYLDVEIQKKDGEVYIYEIEKDYDEDLGVEFTNPIIDQAKSCRNKCMFCF
                     IDQLPEGMRETLYFKDDDSRLSFLQGNFVTLTNMSEEDINNIIKYRISPINISVHTTN
                     PELRQKMISNKFAGKLYGIMKRLADAHIEMNCQIVLCPGINDGKELDRTIKELAQLYP
                     YVNSVAIVPVGITKHRENLVELNIFNDKRASKTIEQIHQMQQKYLEKLGTRFAFLSDE
                     FYILSNSELPGYEEYEGFLQFEDGVGMIRKLKTEIEEYLNILPENILKREKKVSIATG
                     HSAYEFIQSMADAMMDKFKNLQVNVYEIKNKFFGETITVSGLLTAKDLKEQLEDKELG
                     EALYITRSMLKADEEIFLDNIELNQLEELMRIKIIPCLNEGKDFVDKILK"
     gene            complement(13536..13736)
                     /locus_tag="KRL67_000552"
     CDS             complement(13536..13736)
                     /locus_tag="KRL67_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH1521457.1"
                     /translation="MGMDIGLNEYTLAIVTLDKNMKNGGGCPIFYANDEKDLQNKALL
                     MSKCVGGMVHDMTNGTLIIVKH"
     gene            complement(13738..14670)
                     /locus_tag="KRL67_000553"
     CDS             complement(13738..14670)
                     /locus_tag="KRL67_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YIEGIA family protein"
                     /protein_id="HBH1521458.1"
                     /translation="MDRVFFVAVIIGITSRIIMLNLDQKQYPTQPNVLLSQLILAFVA
                     SSLGALLVPALINRSYTSITFLSLAAEQFRQVRANRRNTLQNLEEVQLVKRGAAFIEE
                     IARTYEVRNYMCIVTSFLTIGLYYVILAETKISEGMSLVISSICGLVLAFILKKLLTR
                     KSIGDIADVMPAEISFVDESILKVGDLKGITNIGLKSDREVYINRGVGIQIIPKNKDY
                     ANAGILFDSGQRQAIVYNIYSRLGFYGDYGEPSFVPLPRRNKEDESIMIAYLPVDKNV
                     EKVMEAVKSCPILSSSKGKNLSLKNYKIGKKGSA"
     gene            complement(14715..15647)
                     /locus_tag="KRL67_000554"
     CDS             complement(14715..15647)
                     /locus_tag="KRL67_000554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YIEGIA family protein"
                     /protein_id="HBH1521459.1"
                     /translation="MEESLLNANLFRHSFIVALVVGILCRGLVLRVTDKQYPSRPQDY
                     LEQIIISGLSASLGAIALPALIDKEFAALTFFAVAIQQFQGLAEQERITLKNIDNEEL
                     VPKGDAYVEEIASTYESRSYISLFSALVSSIVYIVFARKYDLSFFYCTILAIVSGAIV
                     GLIFRRFLRRNSIADIADIVPAKISFEGPILMVNGVIITNIGLESTREKYRNECLAIE
                     VIPRDLGAFGIVNDIGQRQAIIHNLFIHMGIDRDVDEVDIVAISKTNLEKSTVVIPYM
                     PILKDIDVMIDVVKSTPIIETSKGKQSDFSRKTL"
     gene            complement(15702..16991)
                     /locus_tag="KRL67_000555"
     CDS             complement(15702..16991)
                     /locus_tag="KRL67_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBH1521460.1"
                     /translation="MEDLNSIYFFIVMLSIAIVILAVRYTITLRKYLKEFIYVSKKVS
                     NNEYHARLNISAKGELGELARNFNHMIKNMDKTLEEVQYKHLQLRSILKSISHGILAI
                     DVNGNILLINDEAKEMIKSEPNQLVEGHNINVVIKEEEILKQILQFIGSKDNETANIT
                     TKDDIVYKIKVDPVYLQNTNNVIIGSIVNIEDITKKVKLENMRSDFVANVSHELKTPL
                     TSISGFVETLKSNENIDVNTRNRFLEIIESESDRLKRLIDDILLLSFVENKDTLLMEN
                     VVVYDVFKEVYEITQHSASEKNIEVLYKCDDKSISILSNKDYIKQVFLNLVDNAIKYT
                     QDNGIVQVVVLKDEEKIVIKVIDNGVGIPKEDVERIFERFYRVDKARSRDVGGTGLGL
                     AITKHIVKSLNGCIKVKSELGIGSEFIVTILQKNFLK"
     gene            complement(17020..17712)
                     /locus_tag="KRL67_000556"
     CDS             complement(17020..17712)
                     /locus_tag="KRL67_000556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBH1521461.1"
                     /translation="MNTKVLVIDDEMHIVELLKFNLEVSNYEVSYSYDGFDGFIKAKE
                     IKPDLILLDWMLPNISGIEVLRKIRSDKDLKNIPVIMLTAKNMENDKVEGLEIGADDY
                     ITKPFSIKELLARISSVLRRYNLTSLGEENNILTTGNLKLDLSKHEVTKGSEKIELTL
                     KEFELLKLLIQNKGKVLSRNYLLDKIWGYEYYGETRTVDVHIRYLRKKIEDEDKSEKY
                     IETIRGVGYKID"
     gene            complement(17727..18488)
                     /gene="pgeF"
                     /locus_tag="KRL67_000557"
     CDS             complement(17727..18488)
                     /gene="pgeF"
                     /locus_tag="KRL67_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan editing factor PgeF"
                     /protein_id="HBH1521462.1"
                     /translation="MMKDYINIKNVQDIKEYVNLSGNCKELDEKLNSVNLVITCKNID
                     AKNREDMLKVCKECDLTFENLTSNSQIHSDIVNIVNKDTIGKRRDGDALITNLEKVPL
                     LLFTADCVPISIIDAKNKAIGLAHAGWRGTFSNIGHKTIKLMSECYKTVPEDLVCIIG
                     PSIGPCCYEVSEDLVEKFNTILTNNDEKFYIIKEGSYYLDLWKINEYMLRCSGVKKEN
                     IINLNLCTSCRADKFHSYRKHNKASERIGTVLQIK"
     gene            complement(18485..18946)
                     /gene="nrdR"
                     /locus_tag="KRL67_000558"
     CDS             complement(18485..18946)
                     /gene="nrdR"
                     /locus_tag="KRL67_000558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor NrdR"
                     /protein_id="HBH1521463.1"
                     /translation="MQCPYCNYKESKVIDSRHTDLKSIRRRRECESCKKRFTTYEKIE
                     TTPLMVIKKDNSREYFDREKIKYGLLKACEKRPVSIEEIESIVVHIENEINKCFIEEI
                     ETKKIGEMVMDKLKELDEVAYVRFASVYRQFKDINTFVNELKSILIEKGDK"
     gene            complement(18968..19231)
                     /locus_tag="KRL67_000559"
     CDS             complement(18968..19231)
                     /locus_tag="KRL67_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlmC/YmxH family sporulation protein"
                     /protein_id="HBH1521464.1"
                     /translation="MIRVSDIMEKEIINVKNGKRMGFIIDIDMDIHEGKVVSFTIFGD
                     GSRSFFSRGGDEEVIFWNDILKIGCDTIIVNIGSELSLDEFEI"
     gene            complement(19335..20108)
                     /gene="sigG"
                     /locus_tag="KRL67_000560"
     CDS             complement(19335..20108)
                     /gene="sigG"
                     /locus_tag="KRL67_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011986856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sporulation sigma factor SigG"
                     /protein_id="HBH1521465.1"
                     /translation="MQVNKVEICGVNTSELPVLKNKQMKELLLQIKNGDEEARQQFVR
                     GNLRLVLSVIKKFNNRGENIDDLFQIGCIGLIKAIDNFDLSQNVRFSTYAVPMIIGEI
                     RRYLRDNNPIRVSRSLKDIAYKALQVRERLIRTNSKEPTVSEIAKELELEVESVVMAL
                     DAIQDPISLFDPVYQDNGDAIFVMDQVQDKKDTDENWLQEISLKEAIKKLNSREKLVL
                     DLRFYKGRTQIEVADEIGISQAQVSRIEKNALKNMRKYV"
     gene            complement(20200..20922)
                     /gene="sigE"
                     /locus_tag="KRL67_000561"
     CDS             complement(20200..20922)
                     /gene="sigE"
                     /locus_tag="KRL67_000561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sporulation sigma factor SigE"
                     /protein_id="HBH1521466.1"
                     /translation="MLRLKERIISFITMLGIKLKIIKPKGIYYMGGANILPPPLKPEE
                     EMELLQKLETDESVKSILIERNLRLVVYISRKFENTGIDVEDLISIGTIGLIKAVNTF
                     KLNKNIKLATYASRCIENEILMYLRKNNKKKTEVSFDEPLNIDLDGNELLLSDVLGTE
                     NDEIYKIIEEEIDRDLLVMALDRLSEREKQIMELRFGLIDKGIEKTQKEVAGMLGISQ
                     SYISRLEKKIISRLQKEMKKFV"
     gene            complement(20947..21819)
                     /locus_tag="KRL67_000562"
     CDS             complement(20947..21819)
                     /locus_tag="KRL67_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-E processing peptidase SpoIIGA"
                     /protein_id="HBH1521467.1"
                     /translation="MFKGFKGGYMVYIEYYVIENLVINYIIISCTSILTKRYNPKKNK
                     LLGASLGGAYSVAYLYPSMNILFTVPFKIIIMSIITLISFRSKSKKDYVHVALVFYLV
                     NIFISGSTYFIIYFTGISHLKISFLIACTYVSCELLKYIYKDLKMLKYIKELTKTINI
                     NLLEKSFCCKALVDSGNLLKDPISQSDVIIVKSSVLEGFIPEKLLNINYEDIDMKKAQ
                     EIIGSLDDKTSGRVRIIPYKHAGSNKTSIIIGLKADYIEVDEQKIGNIILGISNFNDR
                     EYGAIMNPNILPGF"
     gene            complement(22111..23385)
                     /locus_tag="KRL67_000563"
     CDS             complement(22111..23385)
                     /locus_tag="KRL67_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HBH1521468.1"
                     /translation="MIKLKKVLSLVMVGILSVSILAGCSKKEDSKGKEGKEKLVVWSA
                     PLTDHDADAWKPIFEKFEKENNCEIEFQIVPWDNYAEKYATAISAGEGPDIGYMYAEM
                     FPQFIEMGAVEDLTPYLEKSGTSDNYLYLDDAKMMGGIYGLPIEAANPGVLYYNKDIL
                     EKLGEKPPKTWDDFKRICEKATKDTDGDGKIDQWGLAQGWGSKVFGSLNWNWYPYLWQ
                     AGGDIYNDDLKSVKFNNAEGLKAANFINDMKKYIPEDALSKDSNEMIESVFGPGKAAF
                     TIMLSSAATSTFDKSFPDLNWGYVTSLEDKTVGSFASVDDLTLMSACKNKELGFKLLE
                     YMLSKESMEAFHKEVPRAPVAKGEKYQGDPRFEEMVTRDKHMYRPLTVGPHGVEIYEY
                     LWKQLQMMIAGDITPEQALNESAKYANGLLAQ"
     gene            complement(23668..24825)
                     /gene="ftsZ"
                     /locus_tag="KRL67_000564"
     CDS             complement(23668..24825)
                     /gene="ftsZ"
                     /locus_tag="KRL67_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HBH1521469.1"
                     /translation="MLNFDVELEECAQIKVIGVGGGGNNAVNRMVEAQLKGVEFISVN
                     TDKQALYTSKAEYKVQIGEKLTRGLGAGANPEVGKRAAEESKDEIVKLLQGADMVFVT
                     AGMGGGTGTGAAPVVAGLAKEMGILTVGVVTKPFAFEGKIRMKNAEGGIAELKSKVDT
                     LITIPNDRLLQIVQKNTSMLDAFAVADDVLKQGIQSISDLIAVEGLINLDFADVTTIM
                     KDKGLAHMGIGSASGETRAIDAARQAIQSPLLETSIQGAKGVLLNVTGGPNLGLFEVN
                     EASTLVMESCDPEANVIFGASIKEDLGDEIMITVIATGFEGLQNGALDLDTKPKSSIR
                     SSLNTTVKQAVKEIEEEVIAEEKIEPPKKASIIEEDDDESMEIPTFLRRRR"
     gene            complement(25015..25368)
                     /locus_tag="KRL67_000565"
     CDS             complement(25015..25368)
                     /locus_tag="KRL67_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small basic family protein"
                     /protein_id="HBH1521470.1"
                     /translation="MLWVLGGLAFGVVVGYYIPFTYSMDYSIYMSVAILAIMDSIFGA
                     IKSNFEDNYDNVIFITGFIGNAILAILLTYIGEKLGVSLYYVAVLVFGMRLFNNLSII
                     RRYIINNIINKKRKN"
     gene            complement(25467..26141)
                     /locus_tag="KRL67_000566"
     CDS             complement(25467..26141)
                     /locus_tag="KRL67_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF881 domain-containing protein"
                     /protein_id="HBH1521471.1"
                     /translation="MKKKIVILGAFILGVFISTYIKSLDPNRVYITLGQTESIESEVE
                     NTKKEIKNLKETLKNNKKTLNEYKEAKKNGNESIQTLMQKELEEVKELSGYSTVTGPG
                     ITITMRDSERELKDGQNPNDLIIHDIDILRVLNDLKKAGARAISINGERVLATSKIKC
                     SGATITVNDTTYGQPFIIKAIGDIDLLSAAINSPESYAKSLKDIYGINIKVQENNGNK
                     VNLFAK"
     gene            complement(26146..26844)
                     /locus_tag="KRL67_000567"
     CDS             complement(26146..26844)
                     /locus_tag="KRL67_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF881 domain-containing protein"
                     /protein_id="HBH1521472.1"
                     /translation="MKKRLINKSIIIGSIVLGVLVSLQLKIINIENRGMTTFKRGEQL
                     TEELKSLKKEKENLQSEVNSVKKDIETYRGKNNEGEQDILKSEIEKYETLAGYTDVEG
                     TGIKIEINSTKDLGSSDKNDSILYNYDLLLSMINKLNSAQASAISINDQRIVSNTYMY
                     LKEDKLYMNDTVIKEPIIIKAIGDSDTLASALNIKYGIVWEIETYYNAKVKVTKEDNI
                     EINGHSDINKAEGK"
     gene            complement(26867..27607)
                     /locus_tag="KRL67_000568"
     CDS             complement(26867..27607)
                     /locus_tag="KRL67_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsQ-type POTRA domain-containing protein"
                     /protein_id="HBH1521473.1"
                     /translation="MKKRRKLNTNNVMIVLVFILMLSFCICIIVKSDLFDVKKIDVIG
                     NKRVTKSNIMKELNINLNENIFAYNFKDMKNKLIKNPYIENVEIKRKLPNKIIISLKE
                     KEIFAVLKDEDNYCYIDKKGNLLEELRGSNESKKDLIVDVDYSVDDNKSIKFKNYKTK
                     ENVFKTLNYLKEEGIYRKINYVNLKKESNIEMLTRSNIKILLSNDDNLDYNISRVSKI
                     LIDLQNKNTNGGTINLNYGKLAVYSPEG"
     gene            complement(27877..29103)
                     /gene="murG"
                     /locus_tag="KRL67_000569"
     CDS             complement(27877..29103)
                     /gene="murG"
                     /locus_tag="KRL67_000569"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HBH1521474.1"
                     /translation="MKVLLSGGGTGGHVYPAIAIANKIRDEHPDAEIIFVGTEKGIES
                     EIVPKYGFELKTVTVQGFKRKIDFDNVKRVFKLFKGLEQSRKIVKKFKPDIVIGTGGY
                     VSGPVLFNASMGKIPAIIHEQNSFPGVTNKILSKTVTKVLTSFEDSHKRFPEAAEEKL
                     VFTGNPVRKEILLSRKNIARKNLSISDEKRMVLCYGGSGGSRKINDAMRLVIKNMVNE
                     DIAFIFATGKSYYDEFMESISDINLKPYQKVVPYLEDMANALAASDLVIGSAGAISLA
                     EITALGKPSIIIPKAYTAENHQEYNAKSIEKQGAGIAILEKNLTPESLNTAVFKLLGD
                     RELLVDMANASKTIGKPEAIDLIYDEIMKVYNSTQKSTSKKTKKEKVIKEVKEIKKET
                     TPSIEGQAKVIGIKKR"
     gene            complement(29121..30251)
                     /gene="spoVE"
                     /locus_tag="KRL67_000570"
     CDS             complement(29121..30251)
                     /gene="spoVE"
                     /locus_tag="KRL67_000570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein E"
                     /protein_id="HBH1521475.1"
                     /translation="MPKENLKKQIDIRMKTEFDGVVFYTTMLLVFVGIVMVFSASFIQ
                     SSFKHNDAYYFLKKNVIYAVLGFIVMIITSRIDYSFWKKNATAIGAIAVVLLLLVLTP
                     LGIEANGAKRWLGIGALTFQPAEIAKFATIILTAKLIEKNYDKIKSLTKGVVPLLVVP
                     GIFFALIILQPNLSTAGTVILVTFVMIFVAGMDMKIVFAMIGSGAALFAALVIAEPYR
                     LSRVTSFLDPFQDPLGKGYQVIQGLYALGSGGLFGLGLGKSKQKYFYIPEPQNDFIFA
                     IIGEELGLIGCIIVIMLFVVLVYRCVRIALKTSNVFACMVVIGIGAQIGIQAALNIAV
                     ATSSMPATGVALPFISYGGTSLTIFMGAVGIVLNISKHVKIN"
     gene            complement(30303..31658)
                     /locus_tag="KRL67_000571"
     CDS             complement(30303..31658)
                     /locus_tag="KRL67_000571"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HBH1521476.1"
                     /translation="MDLKGKKVLLVGLAKTGISTIKHLDKLGASIIVNDIKDENKLRN
                     ILDELESINDIKYILGHHPEDVDDIDMVVVSPGVPLDLPFILKLKNSGKYIIGEVELA
                     FKLSNNPIFIGITGTNGKTTTTSLVGEIFSRAKRDTYVVGNIGNPVIDTIETSSEESV
                     LVTELSSFQLESIDEFRPKVSAILNITEDHLNRHHTMEKYIEAKANIFMNQTVEDFCI
                     LNYDDEIVKSLADKCNAKVIYFSRTKKVNGGVYLENNDIIIDIDDKIKFLNKDDVSLP
                     GGHNLENCMAAIAIAYVCKIDLEVIRDVLMTFKGVEHRQEFVRNLDNVIYVNDSKGTN
                     PDSTIKAIQSYDRPIILIAGGMDKGSNFDELLETAKSYVKSLVLLGETASNIENCAKN
                     KGFNDIHIVKDMEEAVKTSYEISKSGDIVLLSPACASWDMYESFEVRGKDFKDNVNNL
                     K"
     gene            complement(31677..32645)
                     /locus_tag="KRL67_000572"
     CDS             complement(31677..32645)
                     /locus_tag="KRL67_000572"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HBH1521477.1"
                     /translation="MMLGITELTYTALIAFLIVIIIGPIFIPMLRKFKFGQTVRDDGP
                     QTHLAKNGTPTMGGIIMIVAILITGLTRVKVSHDMAVGLICIAGFGFIGFLDDFIKIK
                     LKRSLGLKAYQKIILQVALSFYVAFYQYTSSSSASQLMIPFTDFVINVGILYIPIMMF
                     IIVAIVNAVNLTDGLDGLASGVTLIVSVFFMLFASSIAGNTEVAVLAAATVGACLGFL
                     GFNSYPARVFMGDTGSMALGGAVVAFSVLTNSVLIIPIIGGIYFAEALSVLIQVGYFK
                     ATRKRFFKMAPIHHHFEQCGWPETRVVFIFWIITVVLAWISIIAVF"
     gene            complement(32686..34059)
                     /locus_tag="KRL67_000573"
     CDS             complement(32686..34059)
                     /locus_tag="KRL67_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HBH1521478.1"
                     /translation="MECLTIKELVLATNGKLIYGDYNDCVSDIVIDSREANAQNAFVA
                     IVGENLDGHTFMKSAYDSGCKTFIKNESNGIKLESSDINLIEVKDTSLALGDISKYYK
                     EKFDIPFIGVTGSVGKTTTRDMIYSAISAKLNILKNEKNLNNHFGVPLTLFNLNKEHE
                     CAVIEMGMSGFNEIKYLVDIVNPKIAVISNIGLSHVEKLGSQEGILKAKMEITSNFDE
                     TNTLIVNGDDKFLSTLKEKEHVYKLKTFGFNKNNDVYCESYTMEEDSLTFICVINGKK
                     EEIFIPTIGEHNIYNAMSAILVGLCLNISLDYIKKGLKNFKGTKMRLDIIKNEKLTII
                     NDSYNASPDSMDAALKILGRYKGRRVAILGDILEMGEISEYGHRLVGKSSMNNTDIII
                     TIGENSVFIGEEAKQLGFNSANIYHFENREDVFNKLNELVKTGDTILVKGSRGMRLEK
                     IVEYLNK"
     gene            complement(34226..36205)
                     /locus_tag="KRL67_000574"
     CDS             complement(34226..36205)
                     /locus_tag="KRL67_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein D"
                     /protein_id="HBH1521479.1"
                     /translation="MRKVKRISKKRLVLVLILACALFFCLVIRTGYLQLMKGNWLSTK
                     ALEQQTRDIPIEPKRGTIYDRNMKELAVSVTKYTVWCKPVEVEDKKEAAEKVAEILDE
                     DYKDIYALISKKNMALVKVKRWIDDDKASQIRDAKLSGIWVAEDNQRYYPYGNFAPYV
                     LGHTSSDATGISGVEMQYDKKLKGKPGKLIVSTDASGREIPQGMEKYYEPVQGNGLVL
                     SIDEVIQHYTEKAVQKAYELNNAKKVTAIAMNPKTGDILALASKPDYDPNDSRTPIYP
                     YYQEELEKYNDKDKIKGYYQMWRNPAVSDTYEPGSTFKLITSSSALEEGVIKDGEKFT
                     CTGSVTVGGRKIKCWRHYRPHGTQEFKQAVQNSCNPVFVELGSRLGVGKMYDYIESFG
                     LMDKTGIDLPGEAKGILYNEKNVGPVELATISFGQSISVTPIQLITAISSIANGGDLM
                     QPRVVKSYTDNKGNITETVKPKKVRSVISKETSKKMLEIAESVVTEGGGKIAYIPGYR
                     LGGKTGTAQKVIDGKYAPGKYICSFVGIAPCDDPQIVVLAIVDEPTGVSAFGSTTAGP
                     IVKEIMNDSLKYLGVKPVYKEEEKAEYEKKQVKVPDVRNLKIGDAVKALEDAKLKPDL
                     DADIELPEDTKVKDIFPKPGVKVNEDSSITLYFEN"
     gene            complement(36217..36564)
                     /locus_tag="KRL67_000575"
     CDS             complement(36217..36564)
                     /locus_tag="KRL67_000575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH1521480.1"
                     /translation="MKKNYKIHNLNEYKNKKKNNYKKRNKKKIGAKCILLIATFSVII
                     LNLCGYSKISEMKYDIYYLQKDLRKKEIVLEELKAKETEKTSIKEIELKAKELLNMDY
                     PKDGQIKYIDVDN"
     gene            complement(37048..37983)
                     /gene="rsmH"
                     /locus_tag="KRL67_000576"
     CDS             complement(37048..37983)
                     /gene="rsmH"
                     /locus_tag="KRL67_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HBH1521481.1"
                     /translation="MEFHHVSVLLNECIENLNIKPDGVYVDCTMGGAGHSKEIVKKLS
                     DKGLFIGFDQDKNAISTAKERLSEYESRVKFVHSNFENIKEELEKIGVYKIDGVLADL
                     GVSSHQLDEADRGFSYMQDAPLDMRMDVRCEFSAYDVVNTYTEDELTKIIKDYGEDNW
                     AKRIAKFIVEERANKPIETTGELVDVIKKAIPKKARIDGPHPAKRTFQAIRIEVNNEL
                     GVITKMINDASSIMNEGGRICIITFHSLEDRIVKNAFKHLASDCICPQHLPICQCDKE
                     SEVKIITRKPILPSEEEIEVNPRSRSAKLRVAEKI"
     gene            complement(38013..38753)
                     /locus_tag="KRL67_000577"
     CDS             complement(38013..38753)
                     /locus_tag="KRL67_000577"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HBH1521482.1"
                     /translation="MDRVAFTLFGIDIMWYGILMACGMILGTLIAIKEAKRVGIKEDD
                     VLNIAIIAIPVGLICARIYYVVFNWSYYAQNMSQIFNFRGGGLAIHGGLIGGILAGYI
                     YTKIKNINFLKMADTVILGMPLAQAIGRWGNFINGEAHGGATNLPWGIMVDGVKVHPT
                     FLYESIWDFGIFIVLLLFRKNKKYEGQVIVTYITLYSIGRFFIEGLRTDSLMLGPLRM
                     AQVISLIGVIGGIIAHVYLSKKNNISEE"
     gene            complement(39151..40437)
                     /locus_tag="KRL67_000578"
     CDS             complement(39151..40437)
                     /locus_tag="KRL67_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HBH1521483.1"
                     /translation="MEKIVNDILKEEVYYEKLQNGLGVYFMPKRGFMKKYAILATNYG
                     SNDLEFVPIGEDKKIRVNEGIAHFLEHKMFEQPDGGDAFDKFSKLGVNANAFTNFTMT
                     AYLFSATENFYESLEHLIDYVQTPYFTDENVEKEKGIIAQEIKMYNDDPDWNVYFNCL
                     KAMYVNYPARIDIAGTVDSIYKITKEELYKCYNTFYNPGNMALFVVGDLDVEKVIDVT
                     KKSNNYKVDKLSKSIERFYPEEPESVKEKEVIEKFPISMPMFNIGFKDSNVGLKGKEL
                     LRKEIVTDILVGMLFKKGSKLYEDLYMQGLINENFGAGFSSQVDYAFSIIAGDSKEPK
                     KVKEIILDYIEKSKKEGLSKEEFERTKKKKIGSFIKCFDSINFIGNSFISYVFKDINL
                     LDYLDVIKDITFEEVEERLKEHFKEDYCVISIVEPK"
     gene            complement(40452..41717)
                     /locus_tag="KRL67_000579"
     CDS             complement(40452..41717)
                     /locus_tag="KRL67_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HBH1521484.1"
                     /translation="MVNTKKIINLGNNINLTLIKTEKFKSNLISLYVQRLLDEKETTK
                     NALIPSIIASGSAKYPSARAISNKLDDLYGSSMGADAVKRGERQVLSFKVINISEKYL
                     DESIFEEVVEFFNEVINNTLVVDGGFKEEYLNIEKENLREKIQSIINDKKEYAQDKCI
                     EAMCKGERYSVSEFGYEDEIDSITSRELYEHYKNILKTSPIDIVVEGNFDEDKVVDII
                     SKNLKFEREEIINIPRADFIKNVDEVKVIDEQMEITQGKLVMGYRANVDYADVDKYYA
                     LVVGSNVLGGGPHSKLFVNVREKESLCYYIFSSIEKYKSIMFISSGIETKDYDKAVEL
                     IKKQVESVKAGDISDEELENSKLALVNSMKSITDNIGGMSDFAFSQSMAKTNSEVQDI
                     ISSIEKVTKKDIVEAIKNIELDTVYFLRN"
BASE COUNT        12896 a         7788 c         4317 g        16798 t
ORIGIN      
        1 taattggtgt tagtatacaa actaaaaaaa taatatcttt ttttagatta tattctttag
       61 actctaattt ataattatta tttattattc catcataatc acttacattt gacttatttt
      121 tatttatatc ttgattttta ttagaaatac tttctctata aggaaaagaa tgttcattgg
      181 taccaaatac tgattttgag tttaaatcat aattaccatc attttctgta attgaacttt
      241 tattgtcaaa tcctttttct ttatcagaat tatttttttt attatcattt ttaggtatat
      301 cattaggatt tttgttttca ttgctaccag tacttgattc tacatcatct tttggagcat
      361 ttggttgttt ttcatcagaa ttatcatttt cattattatt gttttgattg ttattactat
      421 cattattttc attttcattg ttatcatctt gattgttatc atcttgattg ttattatcct
      481 catctctatc tgggtcgtct ctaccgcctc ctccacggtc accatcaata tcatcatttt
      541 cccgttcatc atctggcgtc tcttggtcgt ctccgcctcc cattataaat cctggtttaa
      601 aaactatatt tgaataccct tctcttaatc ctcctaccac ttttactcta aaacaacgca
      661 acttaaaatc ttcaaaaaaa agtatagctt gatctctaat atcctcctgt tcttcagatg
      721 tccaattgat tccatcgctt gaatattcaa caaaaacctt acttgcacta tatggcatat
      781 cataaaacaa agcagctaca tatccatttt ttgtattttg aaattccagt tccaaattgt
      841 ctattggttt tttctctata acagatacat tcaaaatagg aatcacatca tcatttatta
      901 taattttttc tttgtttaac atttctgttt taaactttcc tgttatatta aaattttctc
      961 ccttttctag cttttcataa taatcagtaa aatcccattc tacatctata tcaagttgat
     1021 taccactaga tgtttctaac accatatatt ggggaaattt tatattttct attttatcat
     1081 ctttacttac ttttatttca tcactaaatg caccaattcc cctatggcaa catttcacaa
     1141 tattatagcc agagtcttca gctatagagg gtataagctc attataatca tcaattatat
     1201 aaactttctt gctttctgat ataattgatt gtaccaactt attattatct tttagtaacc
     1261 cattttcatt gttgctacta tatgctttta ttgaggtttc ttctatctca acatcacctt
     1321 tagaataaac acctatagca cgctctccat tcacttcaat atccttattt agtattctaa
     1381 tatcattttc agtataaatt cctattgcat cttttccatt tgcctttatt ttttcaacag
     1441 attcgtagga tgatttaata tgtaagtctc ctccttcttc aacgtataag gctgtcccat
     1501 cagtagcaat tatgttattt ataccaatac ttatacttcc accattttct acatgtatta
     1561 ctccttcttt tcctccttca cctattatat tcaattcatt atcatcaata ataaatctcc
     1621 catgattttt tatcaatatt ttatatttgt tggtatctat tgtaaaatta ctgtcataag
     1681 gaatataaaa tctaaactct tcatctatta ccatatcaca agaaagagta taaactattt
     1741 cttctttatt atttttattt tcttcaaccc atctttcaaa atcttcaaga caattttcct
     1801 cttgtccaaa aactttatgt ggaaaactta taaaacataa aactattata cttataaaaa
     1861 taaatatctc atactttttc atcttacact cctagaaatt ttttagcttt gaaacaatcc
     1921 tgtcgaaaca tatacttcac ccactataat ttttccacaa ttatcaatta ttgtcaattt
     1981 atataagtac ctttattaat tgttataaat aaaaagctac ttactaaagt aaacagacgt
     2041 ttcactttaa taagtagcta tatttttata acaaaatagt tataatatta gaacttcctt
     2101 catttacaat tttttgtaaa gtcttttgca ttttaactct tatctcttct ggcatagtat
     2161 ataatttact ttgtaattgc tcttttacta aatcatgtag tgacttacca aacatatttg
     2221 attcccataa atctgctggt tgctcttcaa atacttccat aagatatttt atcatctctt
     2281 caccttgatt ttgatttcca actattggag aaacttctgt agatatatct gcttttataa
     2341 tatgaagtga tggtgcattg gcttttaact tgataccata ttgttttcct tgtttcatta
     2401 tttctggttc ttccaggcta agttcttcta aagaaggagc tactactcca tatcctttta
     2461 tcttggcatc tattaaagca ctttctattt tatcatactc atttttaact ttagatagtc
     2521 tagttatcag actaagcagt tgataatctc cttcaatttt aaatccactt ttttcttcaa
     2581 ggacattata gaataaatct tgctttgtct gcaaatcaat atttataaca ccttctccta
     2641 gttctacatt atcaacacct gtatcttcca aaaactctaa ctcagaaaat ccttgcataa
     2701 taccttctat atctcttatt ttacctatat ctataatact ttgttttaat gttgttatta
     2761 tgctactttt tatccaatga tttctttcaa gaccttcaac ccattttggt aagttaattc
     2821 ttatttctgt aagtggaaaa tcatataata cagcttccat aacctcctct atatcttctt
     2881 cttccatttc aaccacattc attggaagaa ctggaacttc atatttttct tccaattcac
     2941 ttctaagcat acttgtttcc tcactttttg gacttaacgt atttaaaacc actgcaaatg
     3001 gttttttaag atttttcaat tcttgtataa ctctctcttc tggttctaca tagctttttc
     3061 tgtcaattcc agtaactgag ccatctgtca agacaactat ccctattgta gaatggtctt
     3121 ttataacttt tttagttcct atctctgctg ccttttcaaa tgtcatagct tcttgtgacc
     3181 aaggagtgga tactaatctt tgttttccac cttcctcatg ccctaaagct ccttcaacta
     3241 tgtatccaac acaatccacc attcttactt ttaaagatac tgtgtctttt atttttattt
     3301 ctacgccatc tgctggtaca aattttggtt ctactgtcat tatcgtttta cctgaaccac
     3361 tttggggtat ctcatctctt gttctatcct ttttaaattc attgtctata ttaggtataa
     3421 ccaacttttc cataaatttt cttataaaag ttgattttcc tgttcttaca ggtccaacta
     3481 ctccaatata tatatccccc tgagtccttt tggatatatc ttcgtatatg ttattattca
     3541 tattattcct cctatacatc taatcttatt aaattatatt tagagttaag ggttaatatt
     3601 cttaaaatag aaaaataaag accagcaata ttaatttaat ttacatatag aaatttgtaa
     3661 atttcaatgt aaattaaatt aatattgctg gtccaaatag tagtgtatta tattaactga
     3721 ctttagtagt attttcattt gacttactat tcatctcaat aagttcttta cgtctttctt
     3781 ttgtcatatt catccatata aatgtaaaca ccaatccaat tatggctact atagtaagaa
     3841 ctatgaagta gtacctatat ccagttacac ccggaaatgc atccaatatc ttccctgctg
     3901 ctaaaggaca aagtacttct ggaagatatc ccagtgtagc aactattcct atagcagttc
     3961 ccgatattga taatgaatag ttaccttctt caagtaacga atagtgtaag ccttgaattg
     4021 catacataga tacatatatt gcaacacatc caatcaatag tatataaatc atagatgatt
     4081 ttggtggtgt aaatattaca cctaaaagac ctgcaatcat aagtataaat ccataagata
     4141 taatcttcga tgaaccaact ttatctccaa gtaatccaga agcagctgat gctactggtc
     4201 tacaatactg agctaatatt gagattgcag atgcaaacac cacagtagct ccaaatgctt
     4261 cagttgaata aggcgtaaaa taataaaatg acatattagc tgaatatgaa caaaacatta
     4321 ttattgttat tatccatgta tgaggcattt ttattactgc aaaaaaggct tctttatcaa
     4381 attttgctga tacctctttt tcattatcct ttaatttgaa aaatactaat actccaacta
     4441 ttacatttat tgccgagtat aatactataa tagctgtaag accagattta tcacttactt
     4501 ttgatgaaat atacccaaat agtatcaata ctagagatag atgaattgca ttggtaattc
     4561 cacgaccact ttccataaat ccaaaagctt ttccttgttc attttcagat gctaacattc
     4621 taacagcttt tacaagagct ggccaaaagg taagtattgt tgttattccc catagagcat
     4681 ggattaaaaa cacaacgata tatggaggat aaagtgttaa tacaaaacca gatattcctg
     4741 taataattaa agaacctgaa agcaattttc tggctgaaat acggtctgct aaaaaaccac
     4801 caaaaaggta tgataccatt gcaagtagac cataaatgct tcctaagctt cccatttgtg
     4861 tatttgtaag attaaatgct tgtgcaaaag tatcataata ataattcttt aaatatggta
     4921 aagcatatat aaatgctcct gctacaaaga gtattatcaa gactttgaaa tttttccaaa
     4981 tgcttggttt atttacttca actgacatac ttcctcctta tgtaaatacc cgtaaatttt
     5041 catgataatt aattattgct tattttattg tttattcaat atttcagaat atttattaat
     5101 atattaggtt aatttatcaa taatttatgg tgagttatga gctaaaagta aactctttta
     5161 gctcaccata ataattatat ttttatttat actaacgtcc agagttatta gatatctttt
     5221 tttcatagtt cttccatagc gttaaacctg ttattataac aaaaagacct cctcctatca
     5281 tttcaatctc atatattata ttatctttta aagtctccat aactggtgcc gttgagaaaa
     5341 gtataccaac tacagttact acaagtacca ttctggcaat attgatacaa actgaatcac
     5401 tttttgcaat tgaatatggt ctttcttcgt ttggtcttgt ctttctaagt ctaatataag
     5461 atgtaaaaag caagacgtat ggaaataatg ctgttaatgc tgtcatagta acaagaacat
     5521 tatatatgtt atctactgat ggtaataagt ttgttcctac aagaagtaga ctcactatta
     5581 tagcctgaat tattactgca tttgatggta tattgtgttt atttgtaaca gtcaattgct
     5641 taggaaatac accttctctt acatttccaa aaagcatctt tactggtcct gctatataaa
     5701 gtattaatgc tccaagtact gataaagtta ttccaaatgc tactattctt ataaaccaac
     5761 ttcctatacc taaatcttgt gcaactttag ctaatgcatc tagtatccct gtagatgctg
     5821 caatttcatc tggagggagt atcattgtta ttgctactga acctaatata tagattcctc
     5881 ctattaaaac agccacagtt aatatagctt taggaaaatt tttcttagca ttatccattt
     5941 cagttatgaa attagcagtc gtttcagctc ctgataatgc aaatattata gcagatatag
     6001 aaactaatga attggcatct atttttggta ttatattttg gattgtatat atcgatgctg
     6061 atggagcttt tttgagaata actacagaca taaatgccat cactataagt aatatagcag
     6121 gaattgtaga acctaatgct cctgtattcg taaaaaattt tccaaatgcc atcccacgag
     6181 tacttactaa ggacaacagc caaaatatta tgagtgaaag tgctaaaata aacatcttat
     6241 tgtttgataa atctggattt ccaagcatat atgcaatatt tatagctaaa aatgtcaaga
     6301 aagaagaata ccaaaatatc tttgatgtcc aatttagcca tgacaccata aatccccact
     6361 tctctccata agcttgttta acccattctc caagtccacc atcttttgga tatgttgcag
     6421 ccaactctgc acatataagt gatgcaggaa caaagaatat aaaagcaaac aataaccaaa
     6481 ctggtataga accaagaccc aatccaaaac tagaggcagt agatttagct atccatctaa
     6541 tcccaaatag tgctgatata ttcataaata ctaaatccca aaatccaatt ttctttttca
     6601 ctgtaattcc tccaatttca aatttattca aactcataca caatgtattg atttaaatat
     6661 caagtataag ttcttcaaca cttcttggat aattagttat aattgtagca ccattcttac
     6721 ctactattgc tgtatctgag tgtctaaatc ctccaactcc aggtatatat atccctggct
     6781 ccatagaaaa taccattccc tcttctaata ttaattcatt gtcaaatctt aaatatggct
     6841 cttcatgttc tgataaacct aaaccatgac caattctatg atttgaatat aactctagac
     6901 catactttgc aacaacagct cttgctgctt catccaccat tcttgctggt ataccagctt
     6961 ttattgtatc taatccagct tgttgagcct ctactgcaat cttaaacact tctttttgtc
     7021 tttctgttgg tttacctatg ataaaagttc tttcattttc tgctctatag ttttcatacc
     7081 aaacctgtct actatgtatt actatatctc ctctttgaag tattctagta ttggaatcta
     7141 aatgaggttg cgctgttcta tcaatccctg aacaagtcca gtttgcaaat cctatatatg
     7201 tatcaggata attttcagaa acatatttta gtaaagcatt atcccctgca acgtcaaact
     7261 ccaattcact catcccaact ctaacatttt ctaaagaccc ttttattcct atatcagata
     7321 ggtaacctgc tatcttcaaa aattctattt cttttgcatc ttttacatat ctcatttcaa
     7381 atattttact tccaacatct ttgatagtaa atgacttatc agttatatac tctgaaaaac
     7441 ttgcagatac tatatctttt tctattccaa cagttgttcc tttaggatat ttatttaaaa
     7501 ttatatctaa gtaatgttta tgtgatattc catgctcttt catttctggt atttcataat
     7561 atacatatac attatcaact tttgcaactt ctttagcatg taattcttct aatccaggta
     7621 ctattaattc tattttatct tgagttacta ctgttacaat tggtcgagaa taagttattg
     7681 ctttaaatcc actaatataa aattggttgt ctggttcaaa tattaaagca atttcaatgc
     7741 ttttttcatt catgtacttt ctcaatcttt ctagtctcat attatttgac ataatgcacc
     7801 tctttcttaa tattcagata atttattcaa tacaacaagg atgaatatat tattttaaaa
     7861 ttcttaatat ttttattact tgccgtattt gtatgtaaat taattttatt tgtgattatt
     7921 ttatcataat catgattatt ttgtcaatca cgattttatt ttaaaagatt ttttatattc
     7981 tagtgatttt aaattatcta aaatataacg agtatacatt gtattttgga atattattct
     8041 tgatttatga taagtttaat gataaaatca cgattatata aactatattg attatttgta
     8101 ggtgaataaa atgacaaata aagaattaca aaagaaaagg atattgatgt atttcataga
     8161 agctgctcaa aatattatgg aaaacgaagg tatagaaaat attacacttc gtaaggttgc
     8221 agatatggct ggctataata gttcaactct atacaaatac tttaaaaatt tagaccattt
     8281 aatatcattt gcatctataa aatattttaa agattataac ttgaatattt caagatgtat
     8341 agaaaatgtc aatgatgaat ataagaaata tataattatg tggaaattat tttgtaaaca
     8401 ttcttttgat aatgctcaag ctttttatca ggtatttttt aacttgtcta gtgatgaact
     8461 tagctatata acaaaacaat attatgatat gtttccagaa gatttaggta ttcatgatag
     8521 tgacatatct ttaatgatta caggagaaag tataaaggaa agaaataaga tactattaaa
     8581 caatcttgta gatactggat atataccagc taaagattta gacataatca atgatataat
     8641 tatttctttt tatcaaaatc tattattctt aaaaaagaac acaggaaaaa attctgatga
     8701 tgatgaatta actatgaaaa tgatttcttc tattgaatac ataataaaac atgctaaata
     8761 aaattaaaat atggctgtct caaaatagag ataagccttt tttattgttg aaataataaa
     8821 aatatattta atttcataca aaaaagagtg cctcttaaac taaaaagttc attttgagac
     8881 acccctaaaa caaatttatt atttattata caatagttta tatatcatca cctaaaacta
     8941 cttcttccat ttcatgcttt ttacttctca tcattagttc tattccaact tctttaggat
     9001 ttgaaccttt ctttattaca gaatatatag cattagttat aggcatatct atatctaatt
     9061 tttcagcaac atcatgtgct acttcagtag cagttatacc ttctacaacc atctttactt
     9121 cttttaaagt atcttctaaa ctcatacctt tacctatgag tataccagct cttctatttc
     9181 tactatgcat gcttgtacag gtaactatta aatctccaat acctgaaaga cctgcaaagg
     9241 tagacatatt agctcccata gcaattccaa gtctgctcat ttcactaata cctcttgtca
     9301 taagggcagc ttttgcatta tcaccatacc ctaatccgtc acatatccca gctccaaatg
     9361 ctataatatt ttttaatgct cctccaagtt ctactccaac tatgtctgga ttcgtgtaaa
     9421 ctctaagttt agggctcata aataaatctt gtatcatctg tgctattttt aaatctttag
     9481 aagcaactac tacggtagta ggaatatccc ttgctacttc ttcagcatga gatggcccag
     9541 atagtattac atatggattt tgtggtaatt cttcttcaca gacttgagat aatctaagcc
     9601 cagtcccttt ttcaagacct ttagctacat taacaataac ttgtccttct tttataaatg
     9661 gttttatttt ttggcaagta cttcttacag cctgtgatgg aactgctaaa actattacct
     9721 tacaatcttt tatagttttt tctatatcag tacttatagt tatattgttt ggaaataata
     9781 ctcctggcaa atagtctgta ttttctttag ttctgtttat ttttttagct tgttcctctt
     9841 ttcgagtcca catgctcaca tcattacctt tttttgcaag ccctaaagct aatgcacttc
     9901 cccagcttcc agtacctaat acacatactt tctccatgtt taacaccacc tgagtataat
     9961 atatttacta ttctttcttt tgaccaattt ttctctcggt cccatttaat aatcgcttta
    10021 tatttgctct gtgattatat attactgatg cagttaagaa cagagctact attaaaccag
    10081 ctttattttt taccataatc ataaatattg gtgaacatcc tattcctaca acagaaccca
    10141 atgaaacata ctttgttata gctaccacca atataaatac tgctagacac attagtgtta
    10201 ttactggatt tacagctagc attgccccta gtgaagttgc aacaccttta cctcctctaa
    10261 atcctaaaac tgctggccaa ttatgacctg ctacaacaca tataactgcc agatatccag
    10321 ctaaaagagt atctattcca accaatcttg ctgcaaattc tgatattaaa acagccatta
    10381 cacctttaag aacatcacct aaaaatgtca ttgctcctgc tctttttcca agtgtcctaa
    10441 gtacatttgt cgaacctgca ttaccagaac cttgagtcct tatatcaacc ccagcaattc
    10501 gctttgcaac tatgtatgag gtagatatat ttcctaaaag atatgcaact acagcaataa
    10561 ttatataact aaatatctcc actgaatttc ccccctttag ttatatttta agatatttat
    10621 tttttatttt tttgtctata ctcaaacttt atggaagttc cttcaaatcc aaaattttct
    10681 cttatcttat tctcaaggta tctttgatat gagaagtgag ttaagtcctt atcatttata
    10741 aacaatgtaa ttttaggagg tctaatgtca gtttgagtcc catagtaaat ttttaatctt
    10801 ctacctttgt cagatggtgg ttgatttaac attacagctt ctccaattac ttcattcagt
    10861 gctgaagttg atattctctt agaatgttca tttgacacat attcaactgt atctagtatc
    10921 ttgttcattc tttgattagt ttttgcagaa acaaatacga ttggagcata catcataaat
    10981 ggaaattttt cttttatatc ttttgtgtaa ttgctcatag tcttattatc tttctctatt
    11041 aaatcccatt tatttattac aaatatgcac ccttttcctt catcatgtgc tatacctgca
    11101 acctttgtat cttgttctgt tacaccttct aaggcatcta ttactatcaa tactacatct
    11161 gctctatcta cagcactcat agctcttatt acactgtatt tttctattgt ctcatatatt
    11221 ttactttttc ttctaagccc tgctgtatct attagaagaa atttctgtcc atttttttca
    11281 aaataagtat caattgcgtc tctagtagtt cctgcaattg gacttacaat tactctctct
    11341 tctccaagta tcttattaag tatagaactt tttcctgcat ttggtttacc tgttatagct
    11401 actcttatta tatcttcttc atactctgta tctaatcctg ctggaaaatt ttcaactatc
    11461 tcatctaata agtctccaaa tcccatacta ttggctcctg aaactgcaaa aggcgttcca
    11521 aatcccaact cataaaaatc gtatatatta tcaaattgac tttgactatc aatcttatta
    11581 accactaaga ttactggttt ttttgtcttt ctaagcaatt gtgcaatttc tttatcagca
    11641 gctgttattc cagctttacc atcaactata aaaagtatta catgtgacat gtccattgca
    11701 agcatggctt gatttctcat ttgagataat attatatctt cgctatcggg ttcaattcct
    11761 cctgtatcca ctaatgtaaa atatttatct aaccactcta cttcagcaaa tattctatct
    11821 cttgtaactc caggtgtatt ttcaactatt gatatccttt ttcctgcaaa tttattaaat
    11881 attgtagact taccaacgtt tggtctccca actacagcta caactggcct acttatactc
    11941 attttatttc tccttccatc ttttgttttc tattttaaaa ttttatccac aaagtctttt
    12001 ccttcattca aacaagggat aatttttatt ctcataagtt cttctaattg atttaattca
    12061 atattatcta agaaaatttc ttcatctgct tttagcatac ttcttgttat ataaagagct
    12121 tctcctaatt ctttatcttc aagttgttct tttaaatctt tagctgttaa aagacctgat
    12181 acagttattg tctctccaaa aaacttgttt ttgatttcat atacatttac ttgaagattt
    12241 ttaaacttgt ccatcatagc atctgccata ctctgtataa attcatatgc agaatgacca
    12301 gtagctattg atactttctt ctctcttttt agaatattct caggcaatat atttagatac
    12361 tcttcaatct ctgtttttaa cttacgtatc atacctacac catcttcaaa ctgtaaaaaa
    12421 ccttcatatt cttcatatcc aggcaattca ctattagata gtatataaaa ttcatctgat
    12481 aaaaatgcaa atcgagtacc taatttttca agatatttct gttgcatttg atgtatttgc
    12541 tctatagttt tacttgctct cttatcatta aatatattta attctactag attttctcta
    12601 tgctttgtaa tcccaactgg aactatcgca acactattta catacggata aagttgagct
    12661 agttctttta ttgttctatc cagctccttt ccatcattta tacctggaca taaaacaatt
    12721 tgacagttca tttctatatg agcatctgcc aatctcttca ttataccata taattttcct
    12781 gcaaatttat tgcttatcat tttttgtctt agctctggat tagttgtatg cacagatata
    12841 tttataggac ttattctata ttttataata ttatttatat cttcttcact catatttgta
    12901 agtgtgacaa aatttccttg taaaaaagat aatctcgaat catcatcttt aaaataaaga
    12961 gtttctctca taccttctgg caattggtct ataaaacaaa acatacactt atttctacag
    13021 cttttagctt ggtcaattat cggatttgta aattcaacac ccaaatcttc atcatagtct
    13081 ttttctatct cgtatatgta gacttcgcca tctttttttt gtatttctac atctaaatat
    13141 tcatcactta gtaagaatct atactctatt atatcatgta ttggctgttc attcacagat
    13201 ataagtaaat ctcctacctc tatacctatt tcttctgcaa tactatcttt ataaacctta
    13261 cttataatat tatttacttt ttttacattt acttccaata ttttcaccac tcttttgtat
    13321 tcatcaatgc ttattataat atattttcat atgcaattta tttatttatt atactttata
    13381 atagcatttt taagcatttc atgtcataat aacacatttt aattctaata gtcaatataa
    13441 gtaaatagct ataaaataga ttaaaacaaa taatattcaa atttaagttt cttaaaaatt
    13501 tgaatattat ttgttcttaa aaaaatttta taaatttaat gttttactat gattaacgtc
    13561 ccatttgtca tatcatgaac cattccacct acacactttg acatcaacaa tgccttattt
    13621 tgtaagtctt tctcatcatt tgcataaaat attggacaac ctccaccatt tttcatattc
    13681 ttatcaagtg taactatagc tagggtatat tcatttaaac ctatatccat tcccatctta
    13741 tgcacttcct ttctttccta ttttataatt ttttagtgat aagttttttc ctttagagct
    13801 tgaaagtatt gggcatgact ttacagcttc cattactttt tccacattct tatctactgg
    13861 taaataagca atcattatac tttcatcctc tttatttctt ctaggtaatg gtacaaatga
    13921 aggttctcca taatctccat agaaacctaa ccttgaatat atattgtata ctattgcttg
    13981 tctttgaccg gagtcaaata atattcctgc attagcataa tctttatttt taggaattat
    14041 ttgtattcct acacctctat taatatatac ttctctatcg ctttttagac ctatatttgt
    14101 tatacctttt aaatctccca cctttagtat agattcgtcc acaaaactaa tttcagcagg
    14161 cattacatca gctatgtctc caatactttt tctagtaaga agttttttta atataaaggc
    14221 taacactaat ccacaaattg aacttattac caaactcatt ccttcactta ttttcgtttc
    14281 tgcaagtatt acataataca atcctattgt taaaaaagat gtaactatgc acatataatt
    14341 tcttacttca taggttctag ctatctcttc tatgaatgca gcacctctct ttacaagttg
    14401 aacttcctct aaattttgca atgtatttct tctatttgct ctcacttgtc taaattgttc
    14461 tgctgctaga gataaaaatg ttatggatgt ataagaacga tttataagag ctggaaccaa
    14521 taatgctcct aatgaagaag cgacgaatgc taatataagt tgagataata aaacatttgg
    14581 ttgcgttgga tactgttttt ggtctaaatt caacattata attctagatg ttataccaat
    14641 aataacagca acaaaaaata ctctatccat taaatcactc ctaaaatatt taatttgaaa
    14701 ttttacatgt aaatctacaa agtctttcta gaaaaatcac tctgctttcc ttttgatgtt
    14761 tctatgatag gtgtactttt cactacatct atcatgacat ctatatcttt gagtattggc
    14821 atatatggta taaccacagt acttttttct aaattggtct tagatatagc aacaatatca
    14881 acctcatcta catctctatc tatccccata tgaataaata gattatgtat tatagcttgt
    14941 ctttgcccta tatcatttac tattccaaat gcacctaaat ctcttggtat aacttctatg
    15001 gccagacatt catttctata cttttctctt gtactttcta gacctatatt agttattatt
    15061 acaccattta ccattaaaat aggtccttca aaacttattt ttgcaggaac tatatctgct
    15121 atatctgcta tactgtttct tcttaaaaat cttctaaaaa tcaaaccaac aatagctcct
    15181 gacacaatag ctaagattgt acaataaaaa aagctaaggt catactttct tgcaaaaacg
    15241 atataaacta ttgaagatac tagtgcagaa aaaagactta tataacttct agattcgtac
    15301 gtagatgcta tttcttcaac gtaagcatct ccttttggta ctaattcctc attatcgata
    15361 ttctttagtg ttattctttc ttgttccgca agaccttgaa actgttgtat tgctactgca
    15421 aaaaatgtca aagctgcaaa ctccttatct ataagtgcag gtaatgctat agcacctaac
    15481 gaagctgaaa gcccagatat aattatctgt tctaaataat cttgtggtct tgatgggtat
    15541 tgtttgtctg taactcttaa aactaatcct ctacataata ttcctacaac taatgcaact
    15601 ataaaagagt gtctaaataa atttgcattc aataaacttt cttccatttt ctcagctcct
    15661 tctcttatat agtttgtgca aaaaacaaaa aaaattccct tctatttaag gaaatttttt
    15721 tgtaatattg taactataaa ttcactacca atgccaagtt cactttttac ttttatacat
    15781 ccatttaacg atttgactat atgctttgtt atagccaatc ctaaacccgt tcctcctaca
    15841 tccctacttc ttgctttatc taccctatag aatcgttcaa atatgcgttc tacatcttct
    15901 tttggtatcc ctacaccatt gtctataact ttaattacga ttttttcttc atcttttaac
    15961 actacaactt gtacaatccc attatcttgg gtatacttta tagcattatc aactaggttt
    16021 aagaacactt gctttatata gtctttattg gatagtatac ttattgattt atcatcacat
    16081 ttatataaaa cttctatatt tttttcacta gctgaatgtt gagttatttc atatacctct
    16141 ttaaatacat catatactac aacattttcc ataagaagag tatctttatt ttctacaaat
    16201 gataaaagca atatatcatc aatcaacctt tttaatctgt ctgattcgct ttctattatt
    16261 tctaagaatc tatttctagt atttacatct atattttcat ttgactttag tgtttcgaca
    16321 aatccactta tagatgtaag tggtgtttta agttcatgac ttacatttgc aacaaaatca
    16381 cttctcatat tttcaagttt aactttttta gtaatatctt ctatatttac tatcgaacct
    16441 attattacgt tgttagtatt ctgaagataa actgggtcta cttttatttt atatacgata
    16501 tcatctttag tagtaatatt agcagtttca ttatctttag aaccaataaa ttgaagtatt
    16561 tgttttaata tttcttcttc ttttattaca acattaatat tatgcccttc aactaattga
    16621 tttggttcac tctttatcat ttcttttgct tcatcattta tcagaagtat atttccattt
    16681 acatctatag ccaaaattcc atgagatata ctctttaata tagaccttaa ctgtaaatgt
    16741 ttatactgta cttcttctaa ggttttatcc atattcttta tcatatgatt aaaatttcta
    16801 gctaattcgc caagttctcc tttggctgat atgttaagcc tagcatgata ttcattatta
    16861 cttacttttt ttgagacgta tataaactct tttaaatatt tccttaatgt tattgtgtat
    16921 cttacagcta atataacaat ggctatggac aacataacaa taaaaaaata tatactattt
    16981 aaatcttcca caagataaca ctcctaaaat ataacctttt taatctattt tgtatcctac
    17041 acctcttata gtctctatat atttttccga tttatcttca tcttctattt tttttcttaa
    17101 atatcttata tgaacatcta ctgttctagt ttctccatag tattcatacc cccatatttt
    17161 atccaaaaga taatttcttg aaagtacttt tcctttattt tgaattaata attttaacaa
    17221 ttcaaattcc ttaagagtta actctatctt ttcagagcct ttggtaactt catgttttga
    17281 taaatctagt tttaaattac ctgtagtaag tatattgttt tcttcaccta agctagtcaa
    17341 attatatctc cttaaaacag aactaatcct agctaacaac tcttttatac taaatggctt
    17401 agttatatag tcatcagcac ctatttcaag cccttcaacc ttgtcatttt ccatattttt
    17461 agcagtaagc attataacag gaatattttt taagtcctta tcacttctta tttttctcaa
    17521 aacttctatt ccacttatat ttggtaacat ccaatctaaa agtattaaat ctggtttaat
    17581 ttccttagcc tttataaacc catcaaatcc atcatatgaa taactaactt cgtaatttga
    17641 cacctccaaa ttgaatttta acagttcaac tatatgcatt tcatcgtcta taacaagcac
    17701 tttagtattc attaaaaagc tcctctctat tttatctgta gtaccgttcc tattcgttct
    17761 gatgccttat tatgttttct ataagagtgg aatttatctg ctctacaact agtgcataaa
    17821 tttaaattta ttatattttc ttttttaact ccactacatc taagcatata ttcattaatt
    17881 ttccataaat ctaaatagta acttccctct tttattatat agaacttttc atcattattt
    17941 gtaagaattg tgttaaattt ttctactaaa tcttcagaca cttcataaca acagggtcca
    18001 attgatggtc caattataca tactaaatct tcaggaactg ttttatagca ttcagacatt
    18061 aattttattg ttttatgacc tatattacta aaagtacctc tccaacctgc atgagccagt
    18121 cctatggctt tatttttagc atctataatt gatataggta cacagtctgc tgtaaacagt
    18181 aaaagtggta ccttttccaa gtttgttata agcgcatctc catctcttct ttttcctata
    18241 gtatctttat taactatgtt aactatatct gaatgtattt gagaattact agttagattt
    18301 tcaaatgtca aatcacactc tttacatact tttaacatat cttctctatt ttttgcatct
    18361 atattcttgc atgttataac tagatttaca gagtttaatt tttcatcaag ttctttacaa
    18421 ttaccactta aattaacata ctctttaata tcttgtacat tttttatatt tatataatct
    18481 ttcatcattt gtctcctttt tctatcagta tacttttaag ttcatttaca aaagtattta
    18541 tatctttaaa ttgtctatac acagaagcaa atcttacata ggcaacctca tctaactctt
    18601 tcaatttatc cattaccatc tcaccaattt ttttggtttc aatttcttct ataaaacatt
    18661 tatttatttc attttcaata tgtacaacaa tactttcaat ctcttcaata gaaacaggtc
    18721 ttttttcaca tgcttttagc aatccatatt ttatcttttc tctatcaaaa tattctctac
    18781 tattatcctt tttaatgacc attaaaggag ttgtttctat tttctcatac gtagtaaacc
    18841 tctttttaca tgattcacat tcacgtcttc ttctgataga ttttaaatct gtatgtcttg
    18901 aatctattac ctttgattct ttataattac aataaggaca ttgcatctat attcctcctt
    18961 tgtttaacta tatctcaaac tcatctaagc ttaattctga acctatattt actattattg
    19021 tatcacaacc tattttaagt atatcattcc aaaatataac ttcttcatcc cctcctcttg
    19081 aaaaaaaact tctacttcca tctccaaaaa tggtaaatga gactacctta ccttcgtgaa
    19141 tatccatgtc tatgtctatt ataaacccca tcctttttcc attttttaca tttataattt
    19201 ctttttccat tatatcagaa actctaatca tattgtactc accctttctt taaaaataaa
    19261 tatgtatcga agtaaaaaaa aatgacctat ataggtcatt taaattaaaa taatgtttac
    19321 agtccatgga ttacttatac atattttctc atatttttta aagcattctt ttcaattctt
    19381 gatacttgtg cttgagatat accgatttca tcagcaactt ctatctgagt tcttcctttg
    19441 taaaacctta aatctaatac caacttctct ctactattta atttcttaat agcttcttta
    19501 agtgatattt cttgcaacca attttcatca gtatcttttt tatcttgaac ctgatccata
    19561 acgaatattg catctccatt gtcttgatat actgggtcaa acagagatat tgggtcttgt
    19621 atggcatcaa gtgccattac aactgattct acttctaatt ctaattcctt tgctatttct
    19681 gatactgtag gttctttaga atttgttcta attaatcttt ctctaacttg tagtgcctta
    19741 tatgctatat cttttaatga cctacttact ctaattgggt tattgtctct tagatatctt
    19801 ctaatttctc ctataatcat aggaacagca taagttgaaa accttacatt ttggcttaaa
    19861 tcaaagttat ctatagcttt tataagacca atacaaccta tttgaaataa atcgtctata
    19921 ttttctcctc tgttgttaaa cttttttatt acactcaaga ctaatcttaa attccctctt
    19981 acaaactgtt gtctggcttc ttcatcacca ttttttattt gtaataaaag ttccttcatt
    20041 tgcttatttt taagaactgg aagttctgat gtattaacac cacatatttc aaccttatta
    20101 acttgcatat attttcagtc ctctctcaaa agtattcatc tattgaaatt atttacattt
    20161 ccaatttttt tatacttttt aagaagaatt aatttaacat tatacaaatt ttttcatttc
    20221 tttttgcaac cttgagatta ttttcttttc aagtctagat atgtaggact gtgatattcc
    20281 aagcattcca gcaacttctt tttgtgtttt ttcaatacct ttatcaataa gaccaaatct
    20341 taattccata atttgctttt ctctctcact taatctgtca agtgccataa ccaacaaatc
    20401 tctatctatt tcttcttcaa ttattttgta tatttcatca ttttctgtgc ccaagacatc
    20461 agataataaa agttcatttc catctaaatc tatatttaaa ggttcatcaa atgatacttc
    20521 tgttttcttt ttattatttt ttcttaagta cattaatatt tcattttcta tgcatctcga
    20581 agcataagtt gccaatttta tatttttatt cagtttaaaa gtattaactg cttttatcaa
    20641 acctatagtt cctattgaaa tcaaatcttc tacatctata cctgtatttt caaattttct
    20701 cgatatgtag actactaatc taagatttct ttctattaaa attgacttta cactttcatc
    20761 tgtttctagc ttttgtaaaa gttccatttc ttcttctggt ttcaatggag gaggaagtat
    20821 atttgcccct cccatatagt atattccctt tggctttatt atttttaatt taatacctaa
    20881 catagtaata aagcttatta ttctttcttt caatcgtaac attccttgtc ccccttaaat
    20941 tttcacctaa aatccaggca atatatttgg attcattatt gctccatatt ctctatcatt
    21001 aaaatttgaa ataccaagta ttatatttcc tatcttttgc tcatctactt ctatatagtc
    21061 agcttttaag cctattatta tgcttgtctt attacttcca gcatgtttat aaggtattat
    21121 tctaactcta cctgaagttt tatcatctaa gcttcctata atttcttgtg ctttcttcat
    21181 atctatatct tcataattaa tatttaatag tttttctgga ataaatcctt ctaacacact
    21241 agacttaact attattacat cactttgact tattgggtct tttaacaaat taccactatc
    21301 tactaaagct ttacagcaaa aacttttttc tagtaagttt atatttatag tctttgtaag
    21361 ctccttaatg tacttaagca ttttcaaatc tttataaatg tattttaata attcacaact
    21421 cacataagta catgcaatta agaaagatat ttttaagtga cttattcctg taaaataaat
    21481 tataaaataa gtgcttccag aaataaaaat gtttactaga taaaaaacta acgctacatg
    21541 aacatagtct tttttgctct tacttctaaa tgaaattaaa gttattatac tcatgattat
    21601 tattttaaat ggtacagtaa ataatatatt cattgatgga tacaaataag caacagaata
    21661 ggcacctcct aaactagccc ccaataactt atttttttta ggattatatc ttttagtaag
    21721 aatagaagta caacttataa taatataatt tattactagg ttttctataa catagtactc
    21781 aatatacacc atgtatcccc ccttaaaacc tttaaacata ttataagata gtatgtacaa
    21841 aattttatgt cacttttgat ggtcgaatcc aaaacaaatt ctatttaaaa agaacaatga
    21901 tatatgtctt atactttatg tcgtcaaaag atattttttt aataccaaac aaatagactt
    21961 ttttgtagaa tccaatatat ctaggtagaa gttattcgct ctacttttgg tatatttaca
    22021 taaaaaaaga ctacataatt taataatata tcaagtaaat cttttactta tataaaatca
    22081 aattatatag tctgtaaatt caaatataaa ttattgtgct agtaatccat ttgcatattt
    22141 tgcagattca tttagtgctt gttctggagt tatgtctcca gctatcatca tttgaagttg
    22201 tttccataaa tattcatata tctctactcc atgaggtcca actgtcaatg gtctatacat
    22261 atgtttatct cttgtcacca tttcttcaaa tcttgggtct ccttggtatt tttcaccttt
    22321 agcaactgga gctctaggta cttctttgtg gaaagcttcc attgattctt tgcttagcat
    22381 atattcaaga agtttaaatc caagttcttt atttttacat gctgacatta atgttaaatc
    22441 atctactgaa gcaaaacttc caactgtctt atcttctaat gatgttacat atccccagtt
    22501 taaatccggg aatgatttat caaatgtact tgtggcagca gaggatagca ttatagtaaa
    22561 tgcagctttt cctggtccaa atacactttc aatcatttca ttactatctt ttgataaagc
    22621 atcttctgga atatactttt tcatatcatt tataaagttt gcagctttca agccttctgc
    22681 attattaaat ttaactgatt ttaaatcatc attatatata tctccaccag cttgccataa
    22741 atacggatac cagttccagt ttaagcttcc aaatacttta gaaccccaac cttgtgcaag
    22801 accccactgg tcgatttttc catctccatc agtatctttt gttgcttttt cacaaattct
    22861 tttaaaatca tcccatgttt ttggaggttt ttctcctaat ttttctagta tatctttatt
    22921 gtagtacaat acgcctggat tagcagcttc tataggtaaa ccataaatac cacccatcat
    22981 ttttgcatca tctaagtata gataattatc cgaagttcct gatttttcta aataaggtgt
    23041 taaatcttct accgcaccca tttctataaa ttgaggaaac atctctgcat acatgtatcc
    23101 tatatctgga ccttcgcctg cacttattgc tgttgcatat ttttctgcgt agttatccca
    23161 tggtactatc tgaaattcaa tttcacagtt attttctttt tcaaattttt caaatatcgg
    23221 cttccatgca tctgcatcat ggtctgttaa tggtgccgac catacaacta atttttcttt
    23281 accctctttg cctttagaat cttctttttt tgaacatcct gcaagaattg atactgatag
    23341 aatccctacc ataactaaag aaagaacctt ttttagttta atcatgctta atcccctttc
    23401 agactttaat ttgtcttttt cctcaaatgt ttacatttaa ttcagattta atattaacaa
    23461 acaagactac tcttgtctaa tcataaaaaa ctatactaac tcctataact atattttttt
    23521 tctataccta ttagatttgt ttatactaaa aacttacttt ttaaatttta tcaatattta
    23581 aaaatatttt taagtctagc tacaaactaa ctatttaaat tgaacaaaat aaaaagaagt
    23641 ctattgactt ctttataagc ttacatatta tcttcttctt cttaggaatg taggtatctc
    23701 catgctctca tcatcatctt cttcaattat acttgccttt ttaggaggct ctattttttc
    23761 ctcagcaatt acttcttctt caatttcttt tacagcttgt tttactgttg tatttaatga
    23821 ggaacgtata cttgatttag gtttagtatc taaatctaag gcaccatttt gtaatccttc
    23881 aaatcctgtt gcgattactg taatcattat ttcatcacct aaatcttctt ttatagaagc
    23941 tccaaatata acatttgctt ctgggtcaca ggattccata actaaagttg atgcttcatt
    24001 aacttcaaat aatcctaagt taggaccacc tgtaacatta agaagcactc ctttagctcc
    24061 ttgtattgat gtttctagaa gtggagattg aatagcttgt cttgcagcat caattgctct
    24121 agtttcacca ctagcactac ctatacccat atgagcaagc cccttgtctt tcattatagt
    24181 agtaacgtct gcaaagtcca agtttattaa cccttcaact gctataaggt ctgaaattga
    24241 ttgtatacct tgttttaata catcatctgc aaccgcaaaa gcatctaaca ttgaggtatt
    24301 tttttgtact atctgtaaaa gtctatcatt tggtatcgtt ataagagtat ctacttttga
    24361 tttaagttct gctattccac cttcagcgtt cttcattctt atcttacctt caaatgcaaa
    24421 aggtttggta actacaccta ctgtaagtat ccccatttct tttgccaagc ctgcaactac
    24481 tggagcagct cctgtaccag taccaccgcc cattccagct gttacaaaaa ccatatcagc
    24541 tccttgaagt aactttacta tttcatcttt actctcctca gcagctcttt ttcctacttc
    24601 tggatttgct cctgctccaa gtcctctagt taatttttct cctatttgaa ctttatattc
    24661 agcttttgat gtatataaag cttgtttatc tgtatttact gatataaatt caacgccttt
    24721 tagttgagct tctaccattc tattgacagc attgtttcca ccgccaccta cacctattac
    24781 tttaatttgt gcacattctt ctaattctac gtcaaagttt agcatcataa acccccttct
    24841 ctcatgattc aaagttatcg tatgtattaa ttatatcatt ttcttctttt attttgttaa
    24901 cctttaagta acatattcca caaaaacgta aattttcctt tttaaatttc ttacttatat
    24961 tgtcaaatat tactctttaa gtataaaata atttacgtat atattaaatg ctctttaatt
    25021 tttacgtttt ttattaataa tattattaat aatatatcgt ctaattatag ataagttatt
    25081 aaacaatctc ataccaaaaa ctagtacagc aacatagtaa agagaaactc ctaacttttc
    25141 tcctatataa gtcaataata tagccaatat agcattacct atgaaaccag taataaaaat
    25201 tacattatca tagttatctt caaaattgga ttttatcgct ccaaaaatag aatccattat
    25261 tgcaagaata gctactgaca tatatatgga ataatccata gaatatgtaa atggaatata
    25321 gtaacctact actacaccaa atgctaatcc tcctaaaacc cataacataa atcttcacct
    25381 ctttatttta ctttgaaaca caactacatt acaatccttt ttattaattt tttttacttt
    25441 ttctttattt ttttgtattt ttcgttttat tttgcaaata aattcacttt atttccatta
    25501 ttttcctgaa cttttatatt aattccatat atatccttaa gagatttagc atatgattct
    25561 ggagagttta tagctgcact caataaatct atatctccta tagcttttat tataaatggt
    25621 tgtccatatg tagtatcatt tactgtaata gtagcccctg aacattttat cttacttgtt
    25681 gctagtactc tttccccatt tatagaaatt gctcttgctc cagccttttt taaatcattt
    25741 aaaactctca aaatatctat atcatgtata atcaaatcat ttggattctg cccgtctttt
    25801 aactctctct cactatccct catagtaata gttatgcctg gccctgtaac agttgagtaa
    25861 ccacttaatt ctttaacttc ttcaagctct ttttgcataa gtgtttggat agattcattt
    25921 ccattctttt ttgcttcttt atattcattc aaagtttttt tgttgttttt aagagtttct
    25981 tttaaatttt ttatttcttt ttttgtattt tctacctcac tttcaatact ttcagtttga
    26041 cctaatgtta tgtatactct atttgggtct agtgatttta tataagtcga tatgaaaaca
    26101 cctaatatga atgctcctaa tataactatt ttctttttca tactattatt tcccctctgc
    26161 tttgtttata tcactatgac catttatttc tatattgtct tctttggtca cttttacttt
    26221 agcattataa tatgtttcta tttcccagac aataccatac tttatattaa gtgccgaagc
    26281 caatgtatca gaatcaccaa ttgcttttat tataattggt tcttttatga cagtgtcatt
    26341 catataaagc ttgtcttctt ttaaatacat atatgtatta gaaactattc tctggtcgtt
    26401 tatagatatg gcacttgctt gtgctgaatt taatttattt atcattgaca ataataaatc
    26461 ataattatat agtatactat catttttatc agagcttcct aagtcttttg tagaatttat
    26521 ctcaattttt atgcctgttc cttcaacatc tgtatatcca gctaatgttt catatttttc
    26581 tatttctgac ttaagtatgt cttgttctcc ttcattgttt ttgcctctat aagtctctat
    26641 atctttcttc acactattaa cttctgattg taaattctct ttttcttttt ttaaagattt
    26701 aagttcttct gtaagttgct ctcctctttt aaaagttgtc atccctctat tttctatatt
    26761 aataatttta agttgtaaac taacaagaac acccaataca atacttccta tgattatact
    26821 tttatttata agtctttttt tcaatatatc acttccttac aattgtctat ccttctggac
    26881 tatatactgc caacttccca taattaagat ttatagtccc tccattagta tttttatttt
    26941 gcaggtcaat cagaatttta cttaccctag atatattata atctaagtta tcatcattag
    27001 aaagtaagat ttttatatta cttcttgtaa gcatttctat attgctttct ttttttaagt
    27061 ttacataatt tatttttcta taaattcctt cttcttttaa ataatttaaa gttttaaata
    27121 cattctcctt agttttataa tttttaaatt ttatagattt attatcatca acgctataat
    27181 ctacatctac tatcaagtct tttttgcttt cattagaacc tcttagttct tccaataaat
    27241 ttcctttttt atctatataa caataattat cttcatcttt taatactgca aatatttctt
    27301 tttcttttaa acttataatt atcttattgg gaagttttct ttttatttcc acattttcta
    27361 tatatggatt ttttataagt ttatttttca tatctttaaa attgtatgca aaaatatttt
    27421 catttagatt tatatttagt tctttcatta tatttgattt ggtcaccctc ttatttccaa
    27481 ttacatctat tttctttaca tcaaataaat cactttttac aattatacat atacaaaaag
    27541 ataacataag tataaaaact aaaactatca tgacattatt cgtatttaac tttcttcttt
    27601 ttttcaaata taatcaaccc ctttggttaa taaatacacc tttagagctt gtttttccta
    27661 caaaaaaatt ataatattta aagtcaaatt tttgcatttt attacataac atccaaataa
    27721 agtattaaat tttataaata aatcaatatt tcatcaaaat aaacatagta tactacatac
    27781 tatttatata tttaaatctg tatacataca aataaacccc taatttataa taatttagaa
    27841 aatctaatat attataaatt aggggtaaat atttacttat cttttcttta ttcctattac
    27901 cttagcttga ccttctatac ttggagtcgt ttcttttttt atttctttta cttccttaat
    27961 aactttttct ttttttgttt ttttagaagt tgacttttgt gttgaattgt atactttcat
    28021 tatctcatca tatataaggt ctatggcttc aggcttacct atagtcttac ttgcatttgc
    28081 catatcaacc aaaagttctc tatctcctaa aagtttaaat actgctgtat ttaggctctc
    28141 tggagttaga tttttttcta aaatagcaat tcctgcccct tgtttttcta tactcttggc
    28201 attatactct tgatgatttt cagcagtata agctttaggt attattatag atggctttcc
    28261 aagagccgtt atctcagcta atgaaattgc tcctgcgcta cctataacta aatcacttgc
    28321 tgcaagtgca tttgccatat cttctaagta aggcacaact ttttggtatg gtttaagatt
    28381 tatatcgcta atactttcca taaattcatc ataatagctt ttgcctgtag caaatataaa
    28441 agctatatct tcatttacca tattctttat tacaagtctc attgcatcat ttatttttct
    28501 tgaaccacca ctaccaccat agcaaagtac cattcttttt tcgtcactta ttgatagatt
    28561 ttttcttgca atattttttc ttgatagtaa aatttctttt ctgactgggt ttcctgtaaa
    28621 aactaatttt tcttctgctg cctcaggaaa tcttttatgt gaatcttcaa agcttgtaag
    28681 tacctttgta actgttttag ataaaatctt attagttaca ccaggaaaag aattttgctc
    28741 atgtattatg gctggtattt ttcccataga tgcattaaat aaaacaggtc cactaacata
    28801 tccaccagtt cctataacta tatctggctt aaatttctta acaatctttc ttgattgctc
    28861 caaaccttta aaaagcttaa aaactctttt tacattatcg aaatctattt ttcgcttgaa
    28921 accttgaact gttacagtct ttaattcaaa tccatattta ggaactattt ctgactctat
    28981 tcctttttcc gtcccaacaa aaattatctc agcgtctgga tgctcatctc ttattttgtt
    29041 tgcaatagct atagctggat acacgtgccc tccagttcca ccgcctgata ataaaacttt
    29101 catcaaatca tcatctcctg ttaattaatt ttgacatgct ttgagatgtt taacactatt
    29161 cctacagctc ccataaagat ggtgagtgaa gttcctccat aacttataaa tggtagtgct
    29221 acacctgtag ctggcattga tgatgtcgca actgctatat ttaaagctgc ttgtattcca
    29281 atttgtgcac ctatacctat aacaaccata caagcaaata cattagaagt cttcaaagct
    29341 attcttacac atctgtagac taaaactaca aatagcataa taactattat acaaccaatc
    29401 aatcctaact cttctcctat tatagcaaat ataaagtcat tttgtggctc tggtatataa
    29461 aaatactttt gtttactctt tcctaagccc agaccaaaca atccacctga acctagtgca
    29521 tatagacctt gtattacctg atacccctta ccaagagggt cttgaaaagg gtctaaaaat
    29581 gatgtaacac gactaagtct atagggttca gcaattacta atgcagcaaa taaagcagcc
    29641 ccactcccta tcatagcaaa tacaattttc atatccattc cagcaacaaa tatcattaca
    29701 aaagtaacta gaattacagt acctgctgtt gacaaatttg gctgaagtat tatcaacgca
    29761 aaaaatatcc ctggaactac gagaagtggt acaacacctt ttgtcaaaga ttttatttta
    29821 tcataatttt tttcaattaa ttttgcagtt aatattattg tagcaaactt cgcgatttct
    29881 gcaggttgaa atgttaaagc tcctattccc aaccatcttt ttgctccatt tgcttctatc
    29941 cccaaagggg tcaaaactaa caataataat actactgcta tagctccaat tgctgtagca
    30001 tttttcttcc aaaaactata atctattctc gatgtaataa tcataactat aaatccaagt
    30061 acagcatata ttacattctt ttttagaaaa tagtacgcat cattgtgttt aaatgaagat
    30121 tgtataaaac ttgcactaaa caccataact attcctacaa atactaaaag catagtagtg
    30181 taaaacacta ctccatcaaa ttcagtcttc atccttatgt caatttgttt tttcaaattt
    30241 tctttaggca tccaactaat ctacctccat tcttccattt aagtaagttt agaggtaaga
    30301 gattatttta aattgtttac attatctttg aagtcttttc ctcttacttc aaaactttca
    30361 tacatatccc aacttgcaca agctggtgaa agaagcacta tatctccact ttttgaaatt
    30421 tcataagaag tcttaactgc ttcttccata tcttttacta tatgtatgtc attaaatcct
    30481 ttatttttag cacaattttc tatatttgat gcagtttctc ctaataagac taatgattta
    30541 acatatgact ttgcagtttc aagaagttca tcaaaattac ttcccttatc catacctcct
    30601 gctataagta ttataggtct atcgtaagac tgtatagctt ttatagtaga atcaggatta
    30661 gttccctttg aatcatttac atatattaca ttgtctaagt ttcttacaaa ctcttgtctg
    30721 tgttccactc ctttaaatgt cataagtaca tctcttataa cttctaaatc aattttgcag
    30781 acatatgcta ttgctatagc tgccatacag ttttctaagt tatgacctcc aggtaaactt
    30841 acatcatctt tatttagaaa tttgatttta tcatctatat ctattattat gtcattattt
    30901 tctaaatata ctcctccatt tactttttta gttcttgaaa agtaaattac tttggcattg
    30961 catttatcag ctaaagattt tactatctca tcatcatagt ttaatataca gaaatcctca
    31021 acagtttgat tcataaatat atttgccttt gcttctatgt atttttccat agtatggtgt
    31081 ctatttaagt ggtcctctgt aatatttaat attgcgctta ctttaggtct aaattcatct
    31141 atactctcaa gttgaaaact gcttagttca gttactaaca cagattcttc acttgaagtt
    31201 tctatagtat ctattacagg atttcctata ttaccaacta catatgtatc tcgttttgct
    31261 cttgaaaaaa tttctcctac caaactagtt gtagttgtct ttccatttgt acctgtaatc
    31321 cctataaaaa ttggattatt tgatagttta aaagctaatt ctacctcacc aattatatac
    31381 ttgcctgaat tttttaattt caatatgaat ggtaggtcta gtggtacacc tggagacact
    31441 acaaccatat ctatatcatc tacatcttct ggatgatgtc ctaatatgta ttttatgtca
    31501 tttatagatt ccaattcatc aagtatattt ctcaatttat tttcatcttt gatatcattt
    31561 actattatac ttgcacccaa tttatctaaa tgtttaattg tagatatacc agtttttgca
    31621 agtcctacta ataaaacttt ttttcctttt aagtccattt ttcattcccc ctagccctaa
    31681 aatactgcta ttatacttat ccaagctaaa acaactgtta taatccaaaa aataaataca
    31741 actcttgttt ctggccaacc acactgttca aagtggtggt gtattggagc catcttaaag
    31801 aatctctttc tcgttgcttt aaaatatcca acttgaatca aaactgatag agcctccgca
    31861 aaataaatgc ctccaattat aggtattata agcactgagt tagttagaac tgaaaatgct
    31921 acaactgcac ctcctaatgc cattgaacct gtatcgccca taaacactct agccggatat
    31981 gaattaaatc ctaaaaaacc taaacatgct ccaacagttg ctgccgctaa aactgcgact
    32041 tctgtatttc cagcaataga acttgcaaat agcataaaga atacagaaac tattaatgtt
    32101 actcctgatg ctaagccatc taatccatca gtcaagttta cagcatttac tattgctaca
    32161 attataaaca tcattattgg tatatataat attccaacat ttatcacaaa atctgtaaat
    32221 ggtatcataa gttgtgaagc acttgatgat gatgtatact gataaaaagc aacataaaaa
    32281 gataatgcaa cttgaaggat tattttttga tatgctttta gtccaagtga tctttttagc
    32341 tttattttta taaaatcatc taaaaagcca ataaaaccaa aacctgcaat acatataaga
    32401 cctacggcca tatcatgact aacttttact cttgtaagtc cagttattag aattgcaaca
    32461 atcattataa ttccacccat agtaggtgtt ccgtttttag ctaaatgtgt ttggggtcca
    32521 tcatctctaa ctgtctgacc aaatttaaat tttctaagca ttggtataaa tattggtcct
    32581 attataatta ctattaaaaa cgcaatcaat gcggtgtacg taagctctgt tattcctaac
    32641 atcataaaac tcctctccct tgtcaattaa ctcattagtt taagcttatt tatttagata
    32701 ttcgactatt ttttctaatc tcatgcctct tgagcctttt actaaaatag tgtctcctgt
    32761 ttttactaat tcatttaact tgttaaacac atcttctcta ttctcaaagt gatatatatt
    32821 ggctgaattg aatcctaatt gttttgcttc ttctccaata aacactgaat tttcacctat
    32881 agttattata atatcagtat tattcataga tgattttcca actaatctat gaccatactc
    32941 tgaaatttca cccatttcta gtatatctcc aagtatcgct acccttctac ctttatatct
    33001 tccaagtatc tttaaagcag catccataga atctggacta gcattatatg aatcattaat
    33061 tattgttaat ttttcatttt ttattatgtc tagtctcatt tttgtccctt taaaattttt
    33121 taaacctttt tttatataat ctaaagaaat atttagacat aaaccaacta atatagcaga
    33181 cattgcatta tatatattat gttctcctat tgttggtata aatatttcct cttttttccc
    33241 atttattaca catataaatg ttaaactatc ctcttccata gtataacttt cacaataaac
    33301 gtcattgttt ttattaaagc caaatgtttt taacttatat acatgttctt tttcttttaa
    33361 tgtagataaa aacttatcat ctccatttac aattaaagta tttgtttcat caaagtttga
    33421 agtaatctcc atctttgctt tcaaaattcc ctcttgtgaa cctagttttt ctacatgtga
    33481 taatcctata ttcgatatga ctgcaatttt aggatttact atatccacta aatattttat
    33541 ttcattgaat ccagacatac ccatctctat tacagcacac tcatgttctt tatttagatt
    33601 aaataaagtc aatggaactc caaaatgatt atttaagttt ttttcatttt ttagtatatt
    33661 taattttgca gatatggcag agtaaatcat atctctagta gtagtttttc ccacactacc
    33721 tgtaacacct ataaatggaa tatcaaattt ttctttatag tattttgaaa tatctcctag
    33781 agccaaagat gtatctttta cctctattaa atttatatcc gaactttcta attttattcc
    33841 attactttca ttttttataa aagttttaca tccactatca tatgcagact tcataaatgt
    33901 atgcccatct aaattttctc caactatggc tacaaatgca ttttgggcat ttgcctccct
    33961 gctatctata actatatcac ttacacagtc attataatct ccatatatta acttcccatt
    34021 agttgcaagt actaactctt ttattgttaa gcactccatt attaaactcc tcctttaaaa
    34081 ccttagctat attctcttag caacaaaagg caatgtatac cgttatatat acattgccta
    34141 cgataactct taactttata attatatact atatttctgt ttttttgaag atgtttatgg
    34201 gtatatgtta attatctgtt actttttagt tttcaaaata tagggttata cttgagtctt
    34261 cattaacttt tactccaggt tttgggaata tatcctttac ctttgtatct tctggaagtt
    34321 ctatatctgc atctaagtct ggttttaact tcgcatcctc caatgccttt acagcatctc
    34381 ctatttttag atttcttaca tctggcactt taacttgctt cttttcatac tcagcttttt
    34441 cttcttcttt atatactggt ttaactccta aatattttaa agagtcattc attatttctt
    34501 tcactattgg tcctgctgta gtactaccaa atgcacttac tcctgttggt tcgtcaacaa
    34561 tagcaagaac tactatttgc gggtcatcac aaggtgctat tccaacaaat gaacatatat
    34621 actttcctgg agcatactta ccatctataa ctttttgagc tgtacctgtt ttaccaccaa
    34681 gtctataccc tggtatatat gctatctttc ctccaccttc agttacaact gattctgcaa
    34741 tttctaacat ttttttagat gtttctttag atataacact tcttactttt tttggtttta
    34801 ctgtttcagt tatatttcct ttattgtcag tataagattt tactactctt ggttgcatta
    34861 aatctccacc attagctatt gatgatatag ctgttataag ttgtattgga gttactgata
    34921 tagattgacc aaatgatatt gtagccaatt caactggtcc tacatttttt tcgttgtaca
    34981 gtattccctt agcttctcct ggtaaatcta tacctgtctt atccataaga ccaaatgatt
    35041 ctatataatc atacattttc ccaaccccta atctgcttcc taactcaaca aatactgggt
    35101 tgcaagagtt ttgtactgct tgtttaaact cttgtgttcc atgaggtctg taatgtctcc
    35161 aacatttaat ctttcttcct ccaacagtta cactacctgt acatgtgaat ttttctccat
    35221 ctttaattac tccttcttca agagcactgg aagatgtaat aagcttaaat gtagaacctg
    35281 gttcatatgt atcacttact gctggatttc tccacatttg ataatatcct tttattttat
    35341 ctttatcatt atatttttca agctcttctt gataatatgg atatattggc gttcttgagt
    35401 cattagggtc ataatctggt tttgatgcta aagcaagtat atcacctgtt ttagggttca
    35461 tagctatcgc agtaaccttt tttgcattat ttaactcata agctttctgt actgcttttt
    35521 cagtatagtg ttgaattact tcatctatag acaacacaag accattacct tgtacaggct
    35581 cataatattt ttccattcct tgtggtattt ctcttcctga ggcatcagta cttactataa
    35641 gttttccagg ctttcctttt aactttttat catattgcat ttctacacca gatatacctg
    35701 ttgcatctga agaagtatgt cctaacacat atggtgcaaa attcccatat gggtaatatc
    35761 tttgattatc ttctgctacc catattccac ttaacttagc atctcttatt tgacttgcct
    35821 tatcatcatc tatccatctt tttactttta caagagccat attttttttg cttattaaag
    35881 cgtaaatatc tttataatct tcatccaaaa tctcagctac cttttcggct gcttcctttt
    35941 tatcttctac ttccacaggt ttacaccaaa cagtatactt agttacactt actgctaact
    36001 ctttcatatt tctatcatat atagttcctc ttttgggttc tattggtata tctcttgttt
    36061 gttgttctag tgcttttgta ctcaaccaat ttcccttcat aagttgtagg tatccagtcc
    36121 taataacaag gcaaaaaaac aatgcacatg ctagaataag aacgagtacc agccttttct
    36181 tacttatcct ctttactttt ctcaaatcta ttccccctag ttatccacat ctatatactt
    36241 tatttgtcca tcttttggat agtccatatt gagcaattcc ttagccttta gctcaatttc
    36301 ttttattgat gttttttcag tttcttttgc ctttagttcc tctaatacta tctctttttt
    36361 tcttaaatct ttttgtaaat aatagatatc gtacttcatt tctgagattt ttgaataacc
    36421 acatagattt aatattatta ctgaaaaagt tgcaatcagt aaaatgcatt tagctcctat
    36481 ttttttctta tttctctttt tataattatt ctttttttta tttttatatt catttagatt
    36541 atgtatttta taattttttt tcaaattctc accccgatag tatatagttt aagttttcct
    36601 ctatactata tttgttatta atcatatttc ttcctctaaa atttcatcgc taaattaata
    36661 cactattaca caatctaatt gtattttttt ctatacacaa caaaaaccta taaattcact
    36721 tatgctttta tacgaatcta taggttagat attaatttct atttattttt atcacttcta
    36781 gtatctttag ataatttctt ccataaatat aatatgtaac taactatcta ctctctgatt
    36841 cctaagttct ccattatatt acttaatatc atctatatct atatttttat atctaaattt
    36901 tcattatatg tctcaataac aaagaacatt tcttatagtg ttatttgttt tttaattact
    36961 tttaccagtg caacaaaata tataatactt tttaattaat tataataatt ttattttata
    37021 tataacatta atctttagtc ttataattta tattttttct gctactctta gcttagcact
    37081 tcttgagcgt ggatttactt ctatttcttc ctcactaggt aatataggtt ttctagttat
    37141 tattttcacc tcagattctt tgtcacattg gcatataggt aaatgttgtg gacatataca
    37201 atctgatgct aaatgcttaa atgcattttt cactattcta tcttctaatg aatggaatgt
    37261 tattatacat attcttccac cttcattcat tatagaagaa gcatcattta tcatctttgt
    37321 aattactcca agttcattat ttacttctat tcgtattgcc tgaaaagttc ttttagctgg
    37381 gtgaggtcca tctattctag ctttcttagg gatagctttt ttaattacat ctactaattc
    37441 tcctgtagtt tctattggtt tgtttgctct ttcttcaact ataaatttag ctatacgctt
    37501 agcccagtta tcttctccat aatcttttat tatcttagtt aattcatctt ctgtataagt
    37561 atttacaaca tcatatgcag aaaactcaca cctaacatcc attctcatat caagtggtgc
    37621 atcttgcata taagaaaatc ctctatcagc ttcatcaagt tgatgagatg aaacgcctaa
    37681 atcagctaat acaccatcaa ttttgtaaac tcctattttt tctaattcct cttttatatt
    37741 ttcaaaattg ctgtgaacaa acttcactct actttcatac tcactcaatc tctcttttgc
    37801 tgtgcttata gcatttttat cttggtcaaa ccctataaat aatcccttat ctgacagttt
    37861 ttttactatt tctttagaat gtcctgctcc tcccatagta cagtcaacat acactccatc
    37921 tggctttata tttaaattct ctatacactc atttaaaaga actgatacat gatgaaattc
    37981 catagttacc tcccttaaat tcacttttta aactattctt cactaatatt atttttcttt
    38041 gataaataaa catgtgcaat tataccacca ataactccaa ttagacttat aacttgtgcc
    38101 attctaagtg gtcctaacat taaactatct gttcttaatc cctctataaa aaaccttcct
    38161 attgaatata aagttatata agtaactata acttgacctt catatttttt attttttcta
    38221 aatagtaata aaactataaa tattccaaaa tcccatatgg attcatataa aaaagttgga
    38281 tgtaccttta ctccatcaac cattattccc caaggtaaat tcgtagcacc tccatgtgct
    38341 tctccattaa taaaatttcc ccatcttcca atagcttgtg ctaaaggcat tccaagtata
    38401 actgtatcag ccatttttaa gaagttaata tttttaatct tagtgtatat ataaccagct
    38461 aatattccac caattaaccc tccatgtata gcaagtccac cacctctaaa attaaaaatt
    38521 tgagacatat tttgagcata gtaactccaa ttaaatacaa cataatagat tcttgcacag
    38581 attagtccaa ctgggattgc tattatagct atatttaata catcatcttc tttaattccc
    38641 actctttttg cttcttttat tgctattaaa gttcctaaaa tcattccaca tgccataagt
    38701 attccatacc acatgatgtc tatgccaaac agtgtaaatg ctactctatc cataacgtca
    38761 cctctttgta ttaattgaaa atatttccaa agcaatatgc tataattaca attaattgta
    38821 attacactaa attatgataa cacattaatg ttaattctac aaaaaatatt aaaatttatt
    38881 ctataaatcc ctacactata ttatactaat ctaattactt taaaattttt taaactgaat
    38941 ctctcatgac taaagtcaca agtgtacata gatatttttt aatcaaaaag attgatgcat
    39001 atatacatca acctcttcta taccttatct atgactctta aatttttagc tcaatttata
    39061 caaatatatt ttataaattt gttttatcaa aataaacatt taaaaacata aactaatact
    39121 taattagtta ttcttattat gattaagttt ctatttaggc tctacaatag aaataacaca
    39181 ataatcttct ttaaaatgtt cttttaatct ttcttcaact tcctcaaaag taatatcttt
    39241 aataacatct aaataatcca atagatttat atccttaaaa acataagata taaaactatt
    39301 acctataaag tttatagaat caaagcactt aataaatgac cctatttttt tctttttagt
    39361 tctctcaaac tcttcttttg ataatccttc tttttttgat ttttctatat aatcaagaat
    39421 tatttctttc acttttttag gctctttaga atctccagct attatgctaa aagcataatc
    39481 cacttgagac gaaaatccag ctccaaaatt ttcatttatt aaaccttgca tatacaaatc
    39541 ttcataaagc ttacttcctt ttttaaataa catccctaca agtatatcag ttacaatctc
    39601 tttacgtaaa agttctttcc cttttaagcc aacattacta tctttaaaac ctatgttgaa
    39661 cataggcata gatataggga atttttctat aacctctttt tcttttacac tttctggttc
    39721 ttctggataa aatctttcta tactcttaga gagtttgtct actttgtaat tgtttgactt
    39781 tttagttaca tctataactt tttctacatc taagtcacca acaacaaata aagccatatt
    39841 tcctggatta tagaaagtgt tgtaacattt atataactct tccttagtta tcttatatat
    39901 actatcaaca gtacctgcta tatcaattct agcaggataa tttacataca ttgccttcaa
    39961 gcaattaaag tatacattcc aatcagggtc atcattgtac atttttatct cttgtgctat
    40021 tatacctttt tccttttcaa cattttcatc tgtaaaataa ggtgtttgaa catagtcaat
    40081 tagatgttct aaactctcat aaaaattttc agtagcagaa aataagtagg ctgtcattgt
    40141 aaaattagta aatgcatttg catttactcc taattttgaa aatttatcaa atgcatctcc
    40201 tccatcaggt tgctcaaaca tcttatgctc tagaaaatgt gcaatacctt cattcactct
    40261 aattttctta tcttctccaa ttggtacaaa ttctaagtca tttgaaccat aattagtagc
    40321 caaaatagca tactttttca taaaacccct tttaggcata aaatatacac ctaatccatt
    40381 ttgcaatttc tcataataaa cttcttcttt caatatatca ttaactattt tttccataga
    40441 catctcctcc attaatttct caagaagtat acagtatcaa gctctatatt ttttatagct
    40501 tcaactatat ctttttttgt aaccttttct atactagata ttatgtcttg tacttcagaa
    40561 tttgtctttg ccatagactg agagaaagca aaatcagaca taccacctat attatcagtt
    40621 atagatttca tagaattaac taaagcaagt ttactgtttt ctaattcttc atcagatata
    40681 tcacctgctt ttacactttc tacttgtttt tttatcaatt ctacagcttt atcataatct
    40741 ttagtttcta taccagatga tataaacatg atacttttat acttctctat tgaagagaat
    40801 atatagtaac ataaactttc tttttctctt acattaacaa ataatttaga gtgtggacca
    40861 ccacctaaga cattgcttcc aactactaaa gcataatatt tatctacatc tgcataatcc
    40921 acatttgctc tataacccat aactaactta ccttgagtta tttccatttg ttcatctata
    40981 acttttacct catccacatt ttttataaaa tctgctcttg gtatatttat aatttcttct
    41041 ctttcaaatt ttagattttt acttattata tctacaactt tatcttcatc aaaattacct
    41101 tctacaacta tatctatagg tgatgtttta agtatatttt tataatgttc atataactct
    41161 cttgaagtta tactatctat ttcatcttca taaccaaatt cacttacact atatctctca
    41221 cctttacaca tagcctcaat acatttatct tgtgcatatt cctttttatc atttattatt
    41281 gattgtatct tttctcttaa attctctttt tcaatattta aatattcttc tttaaatcca
    41341 ccatctacaa caagcgtatt atttattact tcattaaaaa attctactac ttcttcaaat
    41401 atactctcat ctaaatactt ttcacttatg ttaattactt taaagctcaa tacttgacgt
    41461 tctccacgtt ttacagcatc agctcccata gatgaaccat ataaatcatc taatttattg
    41521 gatattgctc tagcactagg atatttagcg cttccacttg ctattattga tggtattaga
    41581 gcattttttg tagtttcttt ttcatccaat agtctctgaa catataaact tataagattc
    41641 gatttaaact tttctgtttt tatcaaagta agattaatat tattgcctaa atttattatt
    41701 tttttagtat taaccatatg tacctcctat ttttatttaa acatcaattt taaaattgaa
    41761 atttatttat tttttaatta catatactca catataaaa
//
LOCUS       DAEPXF010000012        41416 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain HB27
            SAMN13376389-rid12479423.denovo.013, whole genome shotgun sequence.
ACCESSION   DAEPXF010000012 DAEPXF010000000
VERSION     DAEPXF010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13376389
            Sequence Read Archive: SRR10518432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 41416)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 41416)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001069624.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001069624.1
            Genome Coverage       :: 315x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/15/2021 01:54:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,165
            CDSs (total)                      :: 4,076
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 89
            rRNAs                             :: 10, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 10 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 19 of 69
            Pseudo Genes (incomplete)         :: 44 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 10 of 69
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41416
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13376389-rid12479423.denovo.013"
                     /strain="HB27"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="China: Hebei"
                     /collection_date="2017"
     gene            complement(97..555)
                     /locus_tag="KRL67_000580"
     CDS             complement(97..555)
                     /locus_tag="KRL67_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH1521485.1"
                     /translation="MKKTIALLLTLCAVMIPFTAFADGNDYEGTGNSQTVELIGQPNS
                     DSPSTKSVVVPGGTANLTAMSGGQLSWRVTSTLGNILGFEGNIDVYKAGKIVSSQFVS
                     FRLAGTTKTGVVSFKGLKKGSYIAKFKGKGVCTGGIILFSSPAEASFTIR"
     gene            complement(590..892)
                     /locus_tag="KRL67_000581"
     CDS             complement(590..892)
                     /locus_tag="KRL67_000581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH1521486.1"
                     /translation="MEVNLHTGKDEFFKSHEVAKNYIGGLYKLIDVYKFSMESVSVLT
                     RIDLDKLNNFYAGEASLNYDELCLIESVIITPLVGAFETAEYNYNMIIKARKERKL"
     gene            complement(1285..1437)
                     /locus_tag="KRL67_000582"
     CDS             complement(1285..1437)
                     /locus_tag="KRL67_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH1521487.1"
                     /translation="MDNFLFNVLASLTASVVVYLISKLFKKAKSHSRAKSDLQVEFKF
                     IFKFKK"
     gene            complement(1557..2291)
                     /locus_tag="KRL67_000583"
     CDS             complement(1557..2291)
                     /locus_tag="KRL67_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HBH1521488.1"
                     /translation="MYYVYKYINQDTEECLYVGKTENICDRHASHLSNKKENWCNKNL
                     RLEYMELDNKYTMDFYEIYLINKLEPKFNITGKGEMDIAKTSFSYNGQWVIYLERDLM
                     SSLASKRYGINYEVNAKMLGIMRKLKKISSNEEIFIGNENIKIRYSFEITLEGIELEP
                     CIMSVAYKTLKESVVGTAISVKREYISENVCLIIDSIFLNEIMKDPLMSKSDIKDLNS
                     QVNDILKIIGVDCEWDKLSDYCNANS"
     gene            complement(2311..3111)
                     /locus_tag="KRL67_000584"
     CDS             complement(2311..3111)
                     /locus_tag="KRL67_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HBH1521489.1"
                     /translation="MYYVYKYIDPATEECLYVGKTEDIYARHSSHLSNKEEDWCTKEL
                     ILKYIELPRRYNMDFFEVYLINELNPIFNKLSKGDMDTEHIFFDYKEEDWSTYSEEDF
                     KEKTKGKRRFRERKIDMKYEVSKKSINFLKRIEKLDAKVEMLYENNVLSVLYNFENMD
                     IEFCENELDINLISYDFETLERFGSCSLISVYPRWKEYTATNKKILINITLEIRIGSL
                     ANLMELMPTFEYKITEIFQQIEDIFHILEVGYTWQELFEIYKGCKYDN"
     gene            complement(3131..4318)
                     /locus_tag="KRL67_000585"
     CDS             complement(3131..4318)
                     /locus_tag="KRL67_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HBH1521490.1"
                     /translation="MEKKEILMQPNNLIKSKYDFTNVENKLFYKILFNAQKQQNSSYV
                     TTISKEELKVFMKNNNDYEHKNIKEILNMFQQSVLEFDYIEETTGKLKTFGSGLINTY
                     ELDHTDQIYTIMMHEVLYNHITDFVKMQKKKNGYTAINLSVLFNFRGAYTQRLYTLFR
                     LWSRENKEVEIKYKLDELRFYLKLKDNVYPEYKYFKQNVLKRAMNEINKKGNMVVSIK
                     EEKRKNRKIDEIIFSVIDYEPRKYFDKDILVEDYIPKKKQDNEVSFSFYIPNENIFTT
                     GTIKLFKEDFKEFDFKKDVYLNAFNKSIAIAFERDNTDIINTKNYDFFKSTLSNKISE
                     VIIHQKDDLKFKEELDKYWDDEDVKKEEREVDYSKDPVRLETVRRNLFDQGIVNEEGY
                     YYK"
     gene            4738..5427
                     /locus_tag="KRL67_000586"
     CDS             4738..5427
                     /locus_tag="KRL67_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HBH1521491.1"
                     /translation="MLENRKQIQKEIYLPIATYCLKDKQYYIKEYGALLIKSAENSDI
                     FNTHKNRILYKNKLDIQEIANELNVNKATIQRNIKKLEKLDFKILKIENTLNGIVYCL
                     PSENNIDLNKFALINYEMLKKIVNKFKSNTIKVYFLLKTITTETNFKPATNSFIAENI
                     GLSSKSKNNLDIITSSVKTLEENKYIETKKVNVYEYDKEKLREVPKIRKVYRICNFDE
                     WKKEIDKNKVY"
     gene            complement(5576..5959)
                     /locus_tag="KRL67_000587"
     CDS             complement(5576..5959)
                     /locus_tag="KRL67_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HBH1521492.1"
                     /translation="MKIKKIPESELLVMMYIWDSEDEEVASTEILKTLGEKYEWKKST
                     MLTFLRRLVGRGFLEVVKKDRFTYYKALIEKEEYLKVETKSFFSFFHRNSFESFISAL
                     HDDEEISANKLKSLEKFIEDWEEDK"
     gene            6546..6701
                     /locus_tag="KRL67_000588"
     CDS             6546..6701
                     /locus_tag="KRL67_000588"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH1521493.1"
                     /translation="MGELKLESKMNCTKNKKKVAISIHQMKKISKKDYVFLTDCIETF
                     YMKIKEV"
     gene            complement(6727..7095)
                     /locus_tag="KRL67_000589"
     CDS             complement(6727..7095)
                     /locus_tag="KRL67_000589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBH1521494.1"
                     /translation="MAEVSKELKAKEIGKRLNELRTLERLSQRAFGERIFLSQDQISL
                     LEKGKRVLTERSINDICREFEVNEEWLRSGKGEIYKDCLADLEIDDDVKEITNKLYEL
                     EQEDRDAILKMIELLQKKNK"
     gene            7267..7428
                     /locus_tag="KRL67_000590"
     CDS             7267..7428
                     /locus_tag="KRL67_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH1521495.1"
                     /translation="MICSNKEKVETALMIDYLKKNEPTEWRTIETIISSTYALSNFKK
                     ENKDIMNQK"
     gene            complement(7729..8103)
                     /locus_tag="KRL67_000591"
     CDS             complement(7729..8103)
                     /locus_tag="KRL67_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009900169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBH1521496.1"
                     /translation="MEKQNLEDNERRQRLKDIRLSKKLKQKDFGKKLGLSLSQISSYE
                     TGHRKIPQRTINDICREFNVNEQWLLHGEGEMFVDITEDLETSEEIKTMIKKIFALED
                     EDREAIEKIIDNAYLKIKNEEL"
     gene            8257..8433
                     /locus_tag="KRL67_000592"
     CDS             8257..8433
                     /locus_tag="KRL67_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH1521497.1"
                     /translation="MNQEKEKANDLLLKLELLKKINPQEFGYISGRIDVNIEKLKQET
                     ERNKSKKIRYRKGA"
     gene            8437..8643
                     /locus_tag="KRL67_000593"
     CDS             8437..8643
                     /locus_tag="KRL67_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH1521498.1"
                     /translation="MTFNKNQIGTAVQLQDIRFDTKVDCIITKVEKNEILVMYYEKET
                     EEIAYKTLTKEDLILDDYKLKLLY"
     gene            8672..9391
                     /locus_tag="KRL67_000594"
     CDS             8672..9391
                     /locus_tag="KRL67_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBH1521499.1"
                     /translation="MDLLKDARKKEWFWLENDLVDREDLSVYEKMAYMVMARYSDDES
                     SCFPSLSLISKKMGCGITTVKKTINSLEEKNLIIKVHRKVKNKKENDTNVYYIMSLKG
                     KSSGDIGHEATDGRSPYDQQVGREATSKKTYNKKTYIKSNTTTQKESKTYLDLSFLDL
                     DIEKVKLTKDEYDKLISKFGKKYIHDKIVSLENYIVNGKGSRYKSHYRALLTWGNGDA
                     SKGIVPEVTKTKNPLSGFKEL"
     gene            9419..9604
                     /locus_tag="KRL67_000595"
     CDS             9419..9604
                     /locus_tag="KRL67_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH1521500.1"
                     /translation="MNEKEETVYLNSSNVKLFLGVRDIKEFEELIENVNERICQLQES
                     INKLAQFNIEFEIKSDI"
     gene            9631..9849
                     /locus_tag="KRL67_000596"
     CDS             9631..9849
                     /locus_tag="KRL67_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH1521501.1"
                     /translation="MADKTVELTEKDLHCIARHLQNEVLEIAFRGNREAPTSCEVCDY
                     FEECKDYFTHIDTFIKLSEMTGVDIFTK"
     gene            complement(9868..10080)
                     /locus_tag="KRL67_000597"
     CDS             complement(9868..10080)
                     /locus_tag="KRL67_000597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH1521502.1"
                     /translation="MNMCKIKTLREKCSLIDDKVEFSGEYKTFPGFAGSKLLECECNK
                     NYICKIQIMTANSNCPLFKKARQLQY"
     gene            10243..10503
                     /locus_tag="KRL67_000598"
     CDS             10243..10503
                     /locus_tag="KRL67_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH1521503.1"
                     /translation="MKEEKLELTEKELHCIARHLQNEVMEMVFRGNREAPTSCEVCDY
                     LQECEGDFTCMYHSFTKLSKITGVKVTAFKYCFPDENPKATI"
     gene            complement(10496..10684)
                     /locus_tag="KRL67_000599"
     CDS             complement(10496..10684)
                     /locus_tag="KRL67_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH1521504.1"
                     /translation="MSQYRVISKECPVINETISLTCEYSAPPGTFGSKLMSTRCDGSH
                     ICDSSSNEECPILKKLLR"
     gene            10822..11139
                     /locus_tag="KRL67_000600"
     CDS             10822..11139
                     /locus_tag="KRL67_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH1521505.1"
                     /translation="MERKKHELTDQDIQRLIEGLRTDPKIREMIFESKGCETRTSTRS
                     KCEQFVEEKLAYRSYGFETPKYYAKLIADIIDILVYKNVPRDRICGVLYDVERVIPLV
                     VTF"
     gene            11161..12489
                     /locus_tag="KRL67_000601"
     CDS             11161..12489
                     /locus_tag="KRL67_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009899669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBH1521506.1"
                     /translation="MNYSLEAEQNILGSFLINDSIGYKIRELKENDFYFESHKIILSC
                     MKRIIDSNKPLDLLLLKNELEKIDRLVDVGGVSYITSLTTIVITTSNIDHYIKIIKEK
                     LLKRQISELANELINNSNSDKSIDELIVDINDLKALVTTSSSVNDNYIDASKIKREKG
                     VHKSIDTGFNKLNNMLDGFRYGTLTILTGKPASGKSTIVNQFIAQAITNGEKAFLYSG
                     ELPSFMAMDWFRKTVANDYHIKEYKSVYGGTYTDIPDYASELISDWIKDKFFLYDEDA
                     ISDEVNLLNTIEHLYLKKGVRFFVLDNLMTIKTGKADKYERQEQIVSNLKNLAKKYNL
                     VIVLVAHPRKNMGDMKPTMYDVSGASEIVNYADYILSTYRVIDEEEETDDTYLLILKN
                     RITGKQNISFKMNFSERRKRLYTTSEELNRDYKYDVNKQYVQVEIPEDVF"
     gene            12860..13246
                     /locus_tag="KRL67_000602"
     CDS             12860..13246
                     /locus_tag="KRL67_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH1521507.1"
                     /translation="MIALEKFAGGVLKEKFNTELQKVLDNIADPNTDFKKTRKISLEI
                     VFKANEDRDLAEVDIKSKSTIVEAKATTTKVLIGKDLETGRVEASEFKNQVAGQLVMD
                     IPDSEETNETEENSASVIDFRNASSK"
     gene            13298..14002
                     /locus_tag="KRL67_000603"
     CDS             13298..14002
                     /locus_tag="KRL67_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH1521508.1"
                     /translation="MIRNAMEYLVKLGKRETVCVDGFKYTTDALEIIKEPKANELEIT
                     TLSGLVDYIKSGIDHKADELLLIQVLTPRKVLLKSPLRKNRDREIYIECVALLPEKRF
                     DRPLDLDSFNIMLQSAFIKNNDRDTLLKIAGNIQESTVKNVGDDGVSQSVTIKTGVAS
                     VSEAIVPNRVKLIPYRTFQEVEQPESEFIFRMSTGPAAGLYEADGGAWKNQAMLNIKA
                     YLQEELKDFKNVNIIA"
     gene            14059..14241
                     /locus_tag="KRL67_000604"
     CDS             14059..14241
                     /locus_tag="KRL67_000604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH1521509.1"
                     /translation="MKMISHMNILERAEFTDKIANAIMSANKEIERGCAFNEAVEIVK
                     QMEYRENKYNEGDIAI"
     gene            14238..14615
                     /gene="ssb"
                     /locus_tag="KRL67_000605"
     CDS             14238..14615
                     /gene="ssb"
                     /locus_tag="KRL67_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HBH1521510.1"
                     /translation="MNNVVLVGRLARDPELRYIPEYGTPVATFALAVDRGYTKKDGTR
                     EVDFIPIEVMGGSAEFCANYLAKGRMVSIQGQIRIEKYEKDGEKKTFTKVRTKIVNAL
                     DHKPKDEEKEISFQVLDDEDIPF"
     gene            14593..15177
                     /locus_tag="KRL67_000606"
     CDS             14593..15177
                     /locus_tag="KRL67_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HBH1521511.1"
                     /translation="MMKIFHFKLDGDYVRSLEERNKLFEDNINLVHFIINRYFKSFLR
                     RYPYLKEDLFQEGYIGLYKTTCCFDESKGKFSTIAFSYISGHLKRFTTGYVKKHYRND
                     IDSFEKCIYRDNYGEEIRIEDRLAGNENVDIENVRVIRSCIKRSEIKDIQKIVCLREK
                     GYTQQEISKVLGTSQTSIYRRLKKLKTEINILGK"
     gene            15206..15364
                     /locus_tag="KRL67_000607"
     CDS             15206..15364
                     /locus_tag="KRL67_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH1521512.1"
                     /translation="MKTKIIGGRENECPICNGNIFKIETLIGIVWQCKDCGCMYQDMS
                     SKESKRGY"
     gene            15367..15693
                     /locus_tag="KRL67_000608"
     CDS             15367..15693
                     /locus_tag="KRL67_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH1521513.1"
                     /translation="MSCVIKCDFCGETIAEILNIKVFRIDDEGNIMSIGKDVCGKCYD
                     RLFGSNLNKKMTNYEKIKNMSKIEMAEFLANGGSGCTSCAYDFQECLGGCLEGRKKWL
                     ESEAMK"
     gene            15693..16229
                     /locus_tag="KRL67_000609"
     CDS             15693..16229
                     /locus_tag="KRL67_000609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HBH1521514.1"
                     /translation="MKKTLIIVGVVISILIMFACLFLVTKNKAIDLEEQVQSATASVE
                     VQEKRRIDLIKNIVDCVKDYAKYENRTLKEVTDMRVSANDESIKEAKLNIQAVAEKYP
                     DLKANENYKQLMSELSITENMIAQHRENYNMQVRNYNKYIRKFPANLILNSMGYEKIE
                     SKYTEYKAKEDAPQNLFD"
     gene            16241..17047
                     /locus_tag="KRL67_000610"
     CDS             16241..17047
                     /locus_tag="KRL67_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH1521515.1"
                     /translation="MKFKTFEITKREFLVSIIIACMMIVLGFFIDDKVQSKFLEDNER
                     YYKATKINNDKDLFDYAMETSAGDSLISGRFKSVDSVSIEELKGDYWFIERTEEKYTM
                     HTRVVTTKDSKGHSHKYTETYWTWDYHDSIEKKCNKFNFMGKEFDAKVITNLSKERLA
                     LKKDIFKNNNLKIKGNYAYINNKRRYYYEVIPKEFNGTMYANLKNNTIANPADKNIKV
                     FKGESIQNVLENNNFNTGFFKVVFWIIWILITTIIIYVFYYLKNDWLEDK"
     gene            17072..17332
                     /locus_tag="KRL67_000611"
     CDS             17072..17332
                     /locus_tag="KRL67_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH1521516.1"
                     /translation="MKCKVYHAIKNIKDANELIDRFETENIFFELNDIDNENVLNGEM
                     DLTDIEDIASLQFILDGDLFIFSSCDEMIFMNKYTQKELGLQ"
     gene            17356..17841
                     /locus_tag="KRL67_000612"
     CDS             17356..17841
                     /locus_tag="KRL67_000612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH1521517.1"
                     /translation="MDLKLISSIPNWKVEDQLAKIDEEVTEFKNAIDTGITKDIIAEG
                     LDVCQTILTMFQVLEIENYITEGVETHNKKLNGRGWDLKEIGDDILNLIKNSMINELI
                     NMNKKLKCLDEKEFNAKIQFLDVDKLWNLCMPLVNLSTIKGVNESIDEMKMGEFLKNI
                     N"
     gene            17861..18118
                     /locus_tag="KRL67_000613"
     CDS             17861..18118
                     /locus_tag="KRL67_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH1521518.1"
                     /translation="MLIEVTENNRELKVGDIVEINGGNKLYMILSLKETIGGYVIINM
                     QNGYGSFGAYKSLSQLELDLKVRGYKLYSSDDYKLQLVPRE"
     gene            18228..18671
                     /locus_tag="KRL67_000614"
     CDS             18228..18671
                     /locus_tag="KRL67_000614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase subunit sigma"
                     /protein_id="HBH1521519.1"
                     /translation="MQSSKKDNLFSSAEGKLYNYKKIKAEIEKIDIDMQMIKNDYRGC
                     SGVEIKEKTSKTYDIKSVIEIETEEKEKKIALKEKEKRHKELIIEKIDNAMKILSEEE
                     KKIVQYKYFSSSRTSWEYVGRMIGFSASKCKQMRFDIIDKIKGLL"
     gene            18814..19359
                     /locus_tag="KRL67_000615"
     CDS             18814..19359
                     /locus_tag="KRL67_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH1521520.1"
                     /translation="MAWNLRSKSFLLSREVLKIKRDITEEISSAAYVPDNLKYYDNVM
                     KETPGMYGRECSLDFIKKKQEKLLKLKKKVKKNYDSNINKIDSYLKILEESIIDESDH
                     VELVVFKLYLQLENVVKVTETVNDLGFRIKTNTYARERKYSTNDITSIITDPFANVVE
                     DLKILVQDRQRKNYHGNKECF"
     gene            19363..20007
                     /locus_tag="KRL67_000616"
     CDS             19363..20007
                     /locus_tag="KRL67_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HBH1521521.1"
                     /translation="MEAHSGQKVNPIKDIEDVFRLLNFLEEWNERNYLLALFGMCTGL
                     RIGDILALKVADVTDVKLDKKGKKIRVSKDWIRVIEEKRDYDREVFLSDVIKSAIENY
                     TQDKPGEEFLFKSGKRKKFNRPIQTRQAGRIIKGAAEKVGIKENVATHSLRKTFARHI
                     YDEEENKTYALELIRKILGHKTIEMTRRYIGIDKDEEVKAITKFTNKLKKRKRN"
     gene            20222..20680
                     /locus_tag="KRL67_000617"
     CDS             20222..20680
                     /locus_tag="KRL67_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBH1521522.1"
                     /translation="MDVNEVEIIDSSYEKGKELTEDQCNMVTLLITGTTVTETARIIG
                     VNRKTIYNWMNKEHVRKEMDRRKQELTNQGNLMILKDLDSYINNIKELASDKSDKRVM
                     LAANQYLINRIYGTPTSTVVQAEDNSVGMGMDATEIEAAIAKIRIRTSKK"
     gene            21235..22974
                     /locus_tag="KRL67_000618"
     CDS             21235..22974
                     /locus_tag="KRL67_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH1521523.1"
                     /translation="MDNLERNIQLIFSYLAKTFIRNGASIKDAEKEANEIIKANSNNL
                     WGVNGLAYQLGKINLEFFCMYFMQDTYLPKEDNAAAPIAKVHHELWEDIQESIIGDGS
                     QQLGRIYPRGTGKSAFGDLATTVWSHCYKHKTYTLICSDIGSTAEKFVKDIKNALLEN
                     EYIKKAFGVLLNDNDRKYICNSTQLELTNKTFIEAISSSSPMRGRKYNNNRPDLIILD
                     DYQSEENVRTEDAREKKFKRFSDDVKYAAQKPVIRNGKTIKRGTTFIALGTLQHKECF
                     YSRLKNLPTWKFKCEKGVLVDNVDELFNSGLWLKFKEILFDFKNTNHLEDAKEFYWEN
                     EKEMKFPILWPSFWDCLDMALSYYENPTSFKQEVQNDVNSIGEKWFKTVRTETREEIE
                     SHTFKKTMLLCDPASAGGSKHDYSAFLVGSESENGLLYGRLAELAKINARTDFDKYID
                     HMIYLLKVYSDITHIYIEKNTFNGADANQLEFKIKNDNVLSYRDIEIINEQQKKNKDD
                     KISTLIPILNKGQMIFAEEDKEFIQQILDFTGQKYSLHDDAPDISSEFINRINNIKVS
                     ETVNIFDRKLLGF"
     gene            22975..23178
                     /locus_tag="KRL67_000619"
     CDS             22975..23178
                     /locus_tag="KRL67_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH1521524.1"
                     /translation="MGGNLLDNRSKFEEVQKTLTKKGKKFNVGIGKDEKGYFAYTHRA
                     RSKSYISKQDIPIKVLKFIESTG"
     gene            23191..24468
                     /locus_tag="KRL67_000620"
     CDS             23191..24468
                     /locus_tag="KRL67_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009899679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HBH1521525.1"
                     /translation="MELDLDLIKDIYDDWNSNKSEYDTMYKYYKGETDAISNYKMVTK
                     RSNNKINTNFLKKFINEEVAYSLANKITYTSRSGDEKIINDLEYYTCHWSKKHDSDLL
                     RYALLFGLCYELYYVKDDEIQVKIIKPTDGCHYENENGEIVCFIREFTKGFEDDTYID
                     VYDKEYIYHFDSNFKEVESPTVNNIFDGNVPISICKRSEELGKNTIFNDIKGLQDAYE
                     TNLSDISNEISDFRNAYLTFSGCNIKEDDLPRMKELGILQVNGDGKIEWLIKDMNDTF
                     VQNTLSSIKENMYEITSHINHNEKMQSNTSSLAVIARLISTEWICSQNNDSIADTLFN
                     RYKLLCIWLNKKYGFDYDYKDIKAKFTPKIPRDDLVVANILSQLGDKLSTETGLSQLS
                     FIDNPHAEIEKAKKEQEIVSEGEILLDESKDYN"
     gene            24446..25207
                     /locus_tag="KRL67_000621"
     CDS             24446..25207
                     /locus_tag="KRL67_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="minor capsid protein"
                     /protein_id="HBH1521526.1"
                     /translation="MNQKIITKLTEDIYKSAENRTKPFYKYQRENRDKLLLEIAKILL
                     THDIINEYLNINNKDKKNLRSKLNKIINEYSNSYDEEVKDIETILSDSSKNKYSNLII
                     LLGVILKIKDVSNINNKVINKIVNNKVDGKHWSDRLWKNKKGIEESLKKEIKSFLNGK
                     TSVNKIEKAIRDKYSTGAGQTSRLIENEVARCQSEVNEYFFKVQGIIKVMYCADLDSR
                     TCSDCSIYNGVIFYVNEDRPSLPRHCHCRCQYIPI"
     gene            25267..25593
                     /locus_tag="KRL67_000622"
     CDS             25267..25593
                     /locus_tag="KRL67_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH1521527.1"
                     /translation="MKVEILGTEYLVIEKLESEDVLLKERAGYCDHSVKEIVVEKINS
                     EEGSLKDLSVYKNEVARHEIIHAFLSESGLKSCSSWATNEEMIDFFAIQFPKIVKVMD
                     KIECLN"
     gene            25619..26221
                     /locus_tag="KRL67_000623"
     CDS             25619..26221
                     /locus_tag="KRL67_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4355 domain-containing protein"
                     /protein_id="HBH1521528.1"
                     /translation="MLKKEVLELLKEIEDDAEIDSLLQSTDLFKSASDKKLTIEEFRE
                     LINNDANFKAIIDSEKNKYHSEALENFKKKDMQTLISAEVLKRTGANETEEQKAIREL
                     RESLNKLEKEKQHAEKISKYKDILVEKKIPTNLIEYLLSDDDDKTNANIEIFENSMKQ
                     YVQSKVDERIKDGSYVPPGKDSAGSLSEIRKQIKQGLNSL"
     gene            26250..27173
                     /locus_tag="KRL67_000624"
     CDS             26250..27173
                     /locus_tag="KRL67_000624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH1521529.1"
                     /translation="MANVLQYVTLFQQELDMQVTVGSTTGWMEKNAGQVIYNGGREVK
                     VPKVTMDGLGDYDRSKGYTQGAVTLEYETMKMEMDRGRTFMLDSMDVNESNFVINASS
                     VMGLFQKQHVIPEIDAYRYSKIFSETAKVDERVKYSYEATVTTILSELKNQLASIQDL
                     SGDIPLIISMSSIIASILEQSSQLDDIEFGDGDIKRIVKSIDNVPIIKVPSKRMKTEY
                     VFRDGKTSGQTEGGFTEGAKALDINWLITPASAPIAVSKTDKPRIFTPDQNQTADAYQ
                     IDYRKYHDLWILDEYLKLCRASVRQAKAAQE"
     gene            27187..27459
                     /locus_tag="KRL67_000625"
     CDS             27187..27459
                     /locus_tag="KRL67_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH1521530.1"
                     /translation="MFALIKNNIIKVVEDENSRDKLISKGFKIYSEEATKENIEKKEE
                     KVKSTSNDLFNLSKPELMEIAKGKNIKGYSTKTKEELIQLLDEYNA"
     gene            27443..27823
                     /locus_tag="KRL67_000626"
     CDS             27443..27823
                     /locus_tag="KRL67_000626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head-tail connector protein"
                     /protein_id="HBH1521531.1"
                     /translation="MSTMLDNVKLVLGIENDRYDKLIILYINKISEMVLEYCSRQELS
                     IALEGFVEEKVISILSSRFEELKKNSGLDNLEKSLKAITRGDTKIEYNALTLQTAENS
                     ISSSSFLTDTDKKFLSPFCIVSMY"
     gene            27835..28182
                     /locus_tag="KRL67_000627"
     CDS             27835..28182
                     /locus_tag="KRL67_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH1521532.1"
                     /translation="MTEADILEMTYLDKMTIIRKLKGWNEETCSNDFSNVVIAENIPC
                     AISRKEEAIVNGDIGSIVVNRKLFCRPEVDIKIGDTINTTYNHGEEAVFKVSKANIYP
                     SHMEIPITEAERI"
     gene            28182..28538
                     /locus_tag="KRL67_000628"
     CDS             28182..28538
                     /locus_tag="KRL67_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 gp10 family phage protein"
                     /protein_id="HBH1521533.1"
                     /translation="MGIDNLGVFEALALDLKLNASNFNRKIKKFTKETGQELAENIRD
                     ETPVKTEKLKKGWEEKQPEYDTYIVKNEVDYATLVNNGHSTKNGGIVPGSFMVEKGIE
                     RTKENMTSAKANLFDW"
     gene            28547..28996
                     /locus_tag="KRL67_000629"
     CDS             28547..28996
                     /locus_tag="KRL67_000629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009899640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH1521534.1"
                     /translation="MLTNRDIIIAVNKRIENEFRYIDIFINEDNIQGFEEACFFVQLL
                     PVSNNIANKVLDTKKIFIDIEYYQKARKNKLNLYDIQSRLEKIFNRNIRVKDRSLIIE
                     NISPSIAKDPVGYKLTFLMTVSYFEEVYFENETFEIMESVDLEIGSD"
     gene            28997..30052
                     /locus_tag="KRL67_000630"
     CDS             28997..30052
                     /locus_tag="KRL67_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath subtilisin-like
                     domain-containing protein"
                     /protein_id="HBH1521535.1"
                     /translation="MGLPEINISFIQAGKTFVKRSGGIVALILKDTKNIGLVEIKEIE
                     DIPENYSPTNLDYIKMAMKGNVYPPNKVLVYTLDTDESIDDALDFLETCEFNYLCMPD
                     ETEQDLPKIKTWIKKMREDNKIKVKAITASNSADYEGIINFTATDIEVEGKKYTSNEF
                     LPRIAGFIAGTPSTQSVTYAEIPEVTNIPKLTRAEANTRINNGELILIKESGAIVIAR
                     GVTSFTTITDSKGDIFKKIKLVDTLDQIHNDIKKIIVKNYIGKTQNTYDNKCLLIVAI
                     QLYLQELEKDGLIDEGSTVEINLDAQRAWLKKQNIDISNMEEQQIKEYNTDTLVFLKA
                     RIKLIDAMEDVYLDISM"
     gene            30068..30508
                     /locus_tag="KRL67_000631"
     CDS             30068..30508
                     /locus_tag="KRL67_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tube protein"
                     /protein_id="HBH1521536.1"
                     /translation="MKAKIDAKRIISGTFGECWLDNKQLTSTKGMEAKVDIDKEEIKL
                     CGQIMTTYKMIGATGKGSITLYKISTLFGEYVEKMLNEGKEFKVTIISKLQDPDSYGS
                     ERIALYGCMFDDLTLADWEAAKVGEIEVPFTFEGFEYLDKIKEK"
     gene            30524..30940
                     /locus_tag="KRL67_000632"
     CDS             30524..30940
                     /locus_tag="KRL67_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HBH1521537.1"
                     /translation="MNTVEKLLNMDAGKLKMPSSTFELYCKKLDDTLEIECKAIDPER
                     FDEIRMNSMDVNSGDVENINIYELKVNTILESCPIFSNMELVKKFSAITPKELVKKLL
                     LSGEIDKLYEEVNKVNGIDAEGDKKRQKERAKDIKN"
     gene            31120..34512
                     /locus_tag="KRL67_000633"
     CDS             31120..34512
                     /locus_tag="KRL67_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HBH1521538.1"
                     /translation="MSDGTRVLQAIIRMRDEASATLRRVAANTRSLHDSTSNASKALN
                     NVQNNLKNVGSTALKTGSAIAAVSGGILALSAKSAIEFESAFAGVKKTVNASDADINT
                     FKKNIMDLSKNMPQTAVEISAVGEAAGQLGIKVGKNASNITNFTKTMVMLGDATNMSS
                     DEAATALARFANIVQMPQKNFDRLGATIVQLGNNLATTESEIVEMALRLAGSGKQVGM
                     SEAQIMSFAGALSSVGIKAEAGGSAFSKVMQQMQLSCEMGGSKLNNFAKVAGMSASKF
                     KESFQKDAAGAIISFVKGLGDAKKHGNSAIKMLNKMGITEIGMTDALLRASGAGDLFN
                     NAIQLGNDAWKENVALTNEANQRYSTTESQLKMMKNQITQVAIIIGNNLLPFIRSAVQ
                     VVSNMANAFSNLPPAFQSTVTGILLAVTTFGLLLATVGIVSLVISKAAGLFAFFIPII
                     LRTKVAFRAFVIGMKPLSAVFLAVMGPMGWTIAAVIALSVAFTVAYAKSEVFRNKVNS
                     LVKSFTEFAKPIVNFCMPALKQLGEKFKELLSAFGPLFASILNLGSVIMKVLGPVLIG
                     VIGFHIAKLVVVLSTLATAFKAVVDTITNVLNGLASILNGIFDLIIGIVTGDSKKMMD
                     GVKQIFDGGTKIIKSIWKGFIDFVTAPIQAIADIADSLFKDKAKWLTDKWRDLRTFLE
                     NPIEATAKISTKTNANIPGSKAADAAGKSTPNTGGLKQTVANLKGSFSDFKTFIKPMT
                     DSISQGVESGKTKFTELQNKILNDVKPALKVLEDSGVNFKDKFKESITSIKDKFNELK
                     NILAGNIKTNIDNLVKAFEPLKPHLDNLKDCFDKVKKAITDFFTPAKQVGDTVNKVSQ
                     PMDSVKKSTDGIKLSFQSLIQALAPVKAWFQGFMPFLSQIGQQLLPSIGIAIAGVVGG
                     IILAFTWVLNTLTAIITTVSGIINGIMSFISGIINVIMGLITGNADQTMDGLKQIFDS
                     GVGVIKSIWNGLVDLVSGVLEPVIDLADSLFKDKVKGVKEAWNKLKEFFHNPIKGMIE
                     IVKSGPSLQIGKGADAAGKSPRKAFGINRVPRNDYLIRAHEGEKLLTKQEANQYKRNS
                     SNGSLFLDKLADTIIVREEADIDKIVRKINKKISLAKVGGVL"
     gene            34559..35035
                     /locus_tag="KRL67_000634"
     CDS             34559..35035
                     /locus_tag="KRL67_000634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4352 domain-containing protein"
                     /protein_id="HBH1521539.1"
                     /translation="MNKKIPALAILIVLSLSLVGCSTNDSKNGDSKPKQNTEEIKNHK
                     IGEVVDLDGAKVSVKNVRKLESDSNVYAVEIEITNTGDTDLESDFLQNYILKTDDNTV
                     GNAINNQELNGELLFDTIHPGDTLKGEIAFELTKDTKPKTIEIAYENVGKYTVFDL"
     gene            35093..35728
                     /locus_tag="KRL67_000635"
     CDS             35093..35728
                     /locus_tag="KRL67_000635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HBH1521540.1"
                     /translation="MEIWLRQANNTFRFPVIPPSFEINGSATINTSNILSVGDIAVFG
                     GLGLKTIELSSFFPNQEYSFCNYNGFPKPYDCVNLIESWMKEGYILRFIITETNINFE
                     CIIADFNYREQDYSRDVYFTLSLKEYRRIQISKVNINNDEKLSSEKDVQLTKGFDTKQ
                     KTHKVVEGDTLFKIAKKYYGNGDLWEKIYKANEDEIKDPSVIKNGWVLIIP"
     gene            35968..37485
                     /locus_tag="KRL67_000636"
     CDS             35968..37485
                     /locus_tag="KRL67_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HBH1521541.1"
                     /translation="MIIDRSKDSNNNNIDFTAKDMGFLLLQSEVSYNFKDKLVEDIAK
                     QVFLENKLPLGNLPKTGVKYTKMFIGVTGYDTIMSVYTEASKTTKKKYMIESNLDKFN
                     VIEKGVVTLNITFEEGFNLINTNFSESMENVKNKVLVVDQYGNKISEKINDSIFKDVG
                     VIMQKVIQQQENSTIDIDGEFKGIEKTCSLKGYGDITCVTGRGVKVKDSYTKLIGLFY
                     IDTDKHTWQNGDYQIELELNFENIMDEKSAGQDEQKEDGSEYTGGTEYSAEFTAYCPR
                     KSEGGDKDCRGKKLDPSKKTCAAPMVGSYEKSYYTKDFLNKHPLMRYGDEIQLVTGVS
                     SRDGTYKVNDNGPAIIIEKDGTYHIDILFGNVEEANNFGRRKGKIIIGGYSGNMTEKA
                     KTVISEARKHLGKPYKWGGNGPSSFDCSGLMVYCFKKVNVNLPRTSSQQSKVGKKVEK
                     NNLQAGDLVFFHNPVSHVGLYVGNGEFLHAPQTGDVVKISKLSNRKDFNTARRVL"
     gene            37499..37831
                     /locus_tag="KRL67_000637"
     CDS             37499..37831
                     /locus_tag="KRL67_000637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2577 family protein"
                     /protein_id="HBH1521542.1"
                     /translation="MADPINEFIGIIRQEGKFHNEPSFFIGKIKSKLPDLKIEINNII
                     LEKEDILIDSWMLDRQIELFDTETSQEHKHEIKNPFIDTFESGNTVIMFKIGEKFAVV
                     SKLVSLDE"
     gene            37824..38258
                     /locus_tag="KRL67_000638"
     CDS             37824..38258
                     /locus_tag="KRL67_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2634 domain-containing protein"
                     /protein_id="HBH1521543.1"
                     /translation="MNNTIFPFMGVPEDYISPDNEELPIFKEFAWDFDKDEKVIENGD
                     FKIVERNEAIKVWIYKTIKTDKYVYLIYDWDYGTDIKNLIGQKYTKGLTESEAKRYIQ
                     EALLTNPYILEVNVTNAEFKDDNLSISLNVKTIYGEEEITFV"
     gene            38251..39303
                     /locus_tag="KRL67_000639"
     CDS             38251..39303
                     /locus_tag="KRL67_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HBH1521544.1"
                     /translation="MYSGQSFSTLNNRTLNNINLPLYKGQGSSLYNIVSPINSELAQL
                     YIELSYIHKRVFIQDNFDDFLDRRVNEFGVYRKLGTEAIGEVIFEGKIGTKIPNGTIV
                     SHSDLLFVVIKDVTIDEDSKLNVSPIQALEVGIKYNLSANTEFKLIEEITGVTKIYNE
                     LDLKGGTEIETDEELKERFYKIQKNQATSGNKAHYQSWALEVEGVYNAKVIPRWDGPG
                     TIKILIYGQNNQSVDNEVLQRCTEHIEEEKPIGPTVTIVTPSTFDINISATLTLENGY
                     DIESIKVVFLDIINSYLIENSREIIYIKIMSLLASIEGVHDIKNLLVNDDVKNIIVDE
                     EKVPAVSSAIFDIEVS"
     gene            39305..39922
                     /locus_tag="KRL67_000640"
     CDS             39305..39922
                     /locus_tag="KRL67_000640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2313 domain-containing protein"
                     /protein_id="HBH1521545.1"
                     /translation="MKLIDYLPSFANNEIDIQIQEALENELLTLIDEKDDLLDQFFID
                     TATWGLDDWESLLGVKINYKLDFDTRRSNIKAKIRGRGITTIKVIKAISEVYTKTNVD
                     VEVFSNLFSFTLSFITNNCSYNTILELDKKIEEIKPAHLEHKFEMILFNENGLYAGAM
                     TSTGETVTIYPYTPKNIECFGEIILASGNNKSAERVTLYPKQEVI"
     gene            39923..40924
                     /locus_tag="KRL67_000641"
     CDS             39923..40924
                     /locus_tag="KRL67_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015984856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBH1521546.1"
                     /translation="MAQAQYYTLLTEIGKAAIANATALGTRVDFAKIKVGDGGGSAYI
                     PTETQTELKNVVWESTLEHVQADEKNPNWVVIQKTITGDTGSFTIREVGVFDSKDQLL
                     AISSYPETYKPAPDSGTVKEILIKIILAVSNTASINLKIDPTVVLATLKDIQDLDSKI
                     DTTKTELTSNIETAKTELNTRIDTENEKQNIKIDQLIAGGSNVASTQTITIDDWVEDA
                     ENGFKATVTHSLLTQRIVVNIIDATTKENVVTNFKIIDDNSIEIRSETRSELNVYVIN
                     GNAETRFINATVDDNRVSEMTTYSSKKIEDRLVNIEEKVNGNLSNIATSVNELITYC"
     gene            40939..>41416
                     /locus_tag="KRL67_000642"
     CDS             40939..>41416
                     /locus_tag="KRL67_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH1521547.1"
                     /translation="MATEWNFDFKAEAQPITLKAGKYKLECWGAHGRVWTGDSPSNGG
                     YSYGELTLKKETTLYVYTGAAGSSNKYEEFTFNGGGLGVNSGGGGATDIRLVNGDWNN
                     EQGLLSRIIVAGGGGGAFSKTPAGKGGGFKGGNSTNDDNSSMLIVPGGTQYDGGRGY"
BASE COUNT        16651 a         4779 c         6959 g        13027 t
ORIGIN      
        1 atcattttgt atataattaa aaacacactt tttataattt tgcataaaaa aagagtgttt
       61 catatatttc taaacactct tttctacttt ttataattat ctaattgtaa aagaagcttc
      121 agctggacta gagaataata taatacctcc tgtacaaaca ccttttcctt taaatttagc
      181 aatatatgaa ccctttttta gccctttgaa acttactaca ccagttttag tagttccagc
      241 taatctaaaa cttacaaatt gagatgatac aattttgcca gccttatata catctatatt
      301 tccttcaaat cctaaaatat ttccaagcgt agatgttact ctccaagata actgcccacc
      361 actcattgca gttaaattag cagtaccacc tggaacaaca acagatttag tcgaaggcga
      421 atctgagttt ggttggccaa ttaattcaac tgtttgtgaa tttccagtac cttcataatc
      481 atttccatca gcaaaagctg taaatggaat cattactgca caaagcgtta gtaataaagc
      541 aattgttttt ttcatttgtt ttccccctat ttttttatat tattctcaat tacagtttcc
      601 tttctttacg tgccttaata atcatattgt aattatactc agcagtttca aatgctccta
      661 ccaaaggagt tataattaca ctttctatta agcacaattc atcatagttt aaactagctt
      721 ctccagcata gaaattattt aacttatcta aatctattct agttaaaacg gaaactgatt
      781 ccattgagaa tttgtaaaca tcaataagtt tatataatcc acctatatag ttttttgcta
      841 cttcatggct cttaaaaaat tcatcttttc ctgtatgtaa atttacttcc ataattccct
      901 ccattgttct tatattttca accttcaaat aatatttact tcttcttcaa aacagaatat
      961 tctatcacgg tagaatattc tgtttttttc attctatcat gatagaataa tattttcaat
     1021 cattttttaa attatattct atttaaataa aatataatat aaaaaagaac acttaatatt
     1081 tttcaagcta atatattaag tgttcttttt tatattttta caaaattata taaaattatt
     1141 ctttaatcaa ttaaagtgtt tttctatttc ttaataataa gaaatgatat aaaattgtat
     1201 cattttaaag attttgtgat ataataaaag caagaagaac tacaatctat ttaggcgtag
     1261 agtggagttc ataataaaca tagtttattt tttgaattta aatataaatt taaattcaac
     1321 ctgtaagtca ctctttgcac gagagtggct ttttgctttt ttgaatagtt tactgattaa
     1381 gtaaactacc acgctagctg ttaaactagc taaaacatta aacaaaaagt tatccatgta
     1441 actcacctcc cctcgaatcg ttgggaggat aatcttttgt acatgaactc cactctatag
     1501 attgtatatt acgctcttct tgctagattt aattatacca tattttgcaa tatcagttaa
     1561 gaatttgcat tacaataatc agaaagcttg tcccactcac aatcaacccc tattatttta
     1621 agaatatcat tgacttggct atttaaatct ttaatatctg atttagacat taaaggatct
     1681 ttcattattt catttagaaa aatactatca atgattaaac atacattttc tgatatatat
     1741 tcacgtttta ctgagattgc tgttccaact acactttctt ttaatgtctt gtatgctaca
     1801 ctcattatac atggttctaa ctcaattcct tctagtgtta tttcaaaaga atatcttatt
     1861 tttatatttt catttccaat aaatatttct tcattgctgc ttatcttctt taattttctc
     1921 attatgccta acatcttagc attcacttca taatttattc catatctctt tgatgccaaa
     1981 ctcgacatta agtctctttc taaataaatt acccactgac cattatagga aaatgatgtt
     2041 ttagcgatat ccatttctcc ttttccagta atattaaatt taggctctag tttatttatt
     2101 aagtaaatct cataaaaatc catagtatat ttattatcta attccatata ctcaagtctt
     2161 aaattcttat tacaccaatt ttctttttta ttacttaaat gactagcgtg tctatcacag
     2221 atattctctg ttttcccaac gtataaacat tcttccgtat cttgattaat atatttataa
     2281 acataataca ttttttattt tctccaattc ttaattgtca tatttacagc ccttatatat
     2341 ttcaaataat tcttgccatg tatatccaac ttctagtatg tgaaaaatat cctctatttg
     2401 ttggaaaatc tcagtaattt tatattcaaa tgttggcatt aattccatta aattagccaa
     2461 agatccaatc ctaatctcta atgttatatt tataagtatt tttttatttg ttgctgtata
     2521 ttctttccat ctgggataaa ctgaaatcaa agaacaactt ccaaatcttt ctaatgtttc
     2581 aaaatcatat gatatcaaat taatatctaa ttcattctca caaaattcta tatccatatt
     2641 ttcaaagtta tatagcacac ttaaaacatt attttcatat aacatttcaa ccttggcatc
     2701 taacttttca attctcttta aaaaattaat agatttttta cttacttcat atttcatatc
     2761 tatctttctt tctctgaatc ttctttttcc ctttgtcttt tccttaaaat cttcttctga
     2821 atatgtactc caatcttctt ccttgtaatc aaaaaagatg tgttcagtat ccatatcacc
     2881 tttacttagt ttattaaata ttggatttag ttcatttatt aaataaactt caaaaaaatc
     2941 catattatat ctacgtggaa gttcaatata ttttaatatt aattctttag tacaccaatc
     3001 ttcttcttta ttactcaaat gagatgaatg ccttgcatat atatcttctg tttttccaac
     3061 gtataaacat tcttctgtag ctggatcaat atatttataa acataataca ttttttattt
     3121 tctccaattt ttatttatag taatatcctt cttcatttac tataccttga tcaaataaat
     3181 ttctcctaac agtttcaagt ctgacaggat ctttgctata atcaacttct ctttcttctt
     3241 ttttaacatc ttcatcatcc caatatttat ctaattcttc tttaaacttt aaatcatctt
     3301 tttgatgaat tataacttca cttattttat ttgaaagtgt tgatttaaaa aaatcataat
     3361 tctttgtatt tataatatca gtattatctc tttcaaatgc tatagcaata gacttattaa
     3421 aagcattcag ataaacatct ttcttaaaat caaactcttt aaaatcttct ttaaatagtt
     3481 taatcgtccc tgttgtaaaa atattttcat ttggtatata aaaactaaaa gacacttcat
     3541 tgtcctgctt ctttttaggt atataatctt caaccaatat atctttatca aaatattttc
     3601 taggttcata atctatcaca gaaaaaatta tttcatctat ttttctattt tttctttttt
     3661 cttcttttat tgaaacaacc atgttgcctt ttttatttat ttcattcatt gctcttttta
     3721 gaacattttg tttaaaatat ttatactctg gatatacatt atcttttaat ttcaaataaa
     3781 atcttaattc atctaactta tattttatct ccacttcctt attttctctg ctccatagtc
     3841 taaataaagt gtataacctc tgagtatatg cacctctaaa attaaataac actgataaat
     3901 taatagctgt atatccattt ttctttttct gcatttttac aaaatcagtt atatgattgt
     3961 atagaacttc atgcatcata attgtataaa tttggtctgt atgatccaat tcatatgtat
     4021 taattaaacc acttccaaaa gtttttaact tgccagttgt ctcttctata tagtcaaatt
     4081 ccaaaacact ttgttgaaac atgttcaaaa tttcttttat atttttatgc tcataatcat
     4141 tattgttttt cataaatact tttagttctt cttttgatat agttgtaaca taagatgaat
     4201 tttgctgttt ctgagcatta aaaagtattt tgtaaaataa tttattttcc acatttgtaa
     4261 aatcatattt actttttatt aaattatttg gttgcattaa aatttctttt ttttccaaaa
     4321 tctatcacct catataaatt ataataccta atttataaaa aggtcaacta tttttgaaag
     4381 acgctcacct ttgaccctta aaatgatgac ctttgaccct taaaatgatg acctttgacc
     4441 cttaaaatga tgacctttcg acccttaaaa tgatgacctt tgacccttaa aatgatgacc
     4501 tttgaccctt aaaatgatga cctttcatct ctgaaaccta gatataccaa ttattatagc
     4561 ttgtctaaaa actattaaag actttaaaaa ctgttttaaa aactattaaa gactagaaaa
     4621 gatgttctga actattcttt ttttatatta aaaaacttta gtatttattt ttctagtata
     4681 ctttaaacac ttactaaaaa taatttctta taaaagattg gagatgaaga attttttatg
     4741 cttgaaaaca gaaaacaaat tcagaaagaa atttacttac caatagcaac atattgttta
     4801 aaagataagc aatattatat aaaagaatat ggagcactat taataaagag tgctgaaaat
     4861 agtgacattt ttaataccca taaaaataga attttgtata agaataaact tgatattcaa
     4921 gaaatagcaa atgaactgaa tgtaaataaa gcaacaatcc aaagaaatat aaaaaaacta
     4981 gagaagttag attttaaaat tctaaaaata gaaaataccc taaatggaat tgtatattgt
     5041 ttgccgtcag aaaataatat tgatttaaac aaatttgcat tgataaatta tgaaatgctt
     5101 aaaaaaatag tcaataaatt taaatcaaat acaataaaag tatatttttt acttaaaaca
     5161 atcactacag aaactaattt taaacctgca actaatagtt ttatagcaga aaatatagga
     5221 ctaagttcta aaagtaaaaa taatttagat atcataactt cttctgttaa aactttagaa
     5281 gaaaataaat acattgaaac aaaaaaagta aatgtatatg aatatgacaa agaaaaactt
     5341 agagaagttc caaaaataag aaaagtttat cggatttgta attttgatga gtggaaaaag
     5401 gaaattgata aaaataaagt ttattaaata tagcttaaaa gcatttaaag gcttgtctga
     5461 cacgttttaa ttgcaaactt gatattattt acctaaaaag atacaaattt gcttaaaaat
     5521 ggaaatagaa ggtcaaatat aaaaatagag aaccaaataa aattatattg gctctctatt
     5581 tatcttcttc ccaatcctca ataaactttt ctaaactttt taatttattt gcagatattt
     5641 cttcatcatc atgtaatgca cttataaaac tttcaaagga atttctgtga aagaaactaa
     5701 agaaactttt agtttcaact tttaaatatt cttctttttc tattaatgct ttataatatg
     5761 taaatctatc tttcttaaca acttctaaaa atcctctacc aactaacctt cttaaaaaag
     5821 ttaacatagt tgattttttc cattcatatt tttcacctaa tgtttttaaa atttcagtag
     5881 atgcaacttc ttcatcttca ctatcccaaa tgtacatcat taccaaaagt tctgattctg
     5941 gaatcttttt tatcttcata aagcacacca ccttttcaaa agtataacta aaatactata
     6001 acaatagagt taactataca atactaaatg tctaaaaatg ttattccaat ttttaccaat
     6061 taatttttct ttaaaaaaaa tttaagcagg agtaaaaaaa aataaaaaat tatcaaaatt
     6121 tatcaatata aaaagaaata taagtataaa tttttctctt tgtatattga ttgtatacaa
     6181 ttctattttt gtaaaattaa attttacaaa ataatcaaaa atggtaaatt ttgacatttg
     6241 cctaatgtca aattaggtgt tacaattgta acataaagaa gatatatatc gaaataatac
     6301 atataaaata catataaaac tacgtacgta aaatattttt aaaaataatc aaaaatctta
     6361 attttcaata ttgaatacaa ttaaattttc aaactcatct agggcatgac atacaaaagc
     6421 aaaaaacatt aatttagtat ctgataaaaa tttaaaaatt ataaaagggg gaagtctgtt
     6481 atttttcaga agcaatgtaa ctgtaaaaat ataaatcaga atattttgaa aatatggggg
     6541 attctatggg agaattaaaa cttgaatcaa aaatgaattg cactaaaaat aagaaaaaag
     6601 ttgctatatc tatacatcaa atgaaaaaaa tttctaaaaa agattatgtt tttttaacag
     6661 attgcataga aacattttat atgaaaataa aagaggtttg ataaattttc aaacctcttt
     6721 ttattgctat ttgtttttct tttgcaataa ttctatcatt ttcaagatag catctctatc
     6781 ttcttgttct aattcataaa gtttgttagt tatttctttt acatcatcat caatttctaa
     6841 gtcagctaaa caatctttat aaatttcacc ttttccactt cttagccatt cctcatttac
     6901 ttcaaattct ctgcatatgt cgttaatact tctttctgtc aaaactcttt ttcctttttc
     6961 caataaagaa atttgatctt gtgataagaa aattctttcc ccaaacgctc tctgcgaaag
     7021 tctttcaaga gttcttaact catttaatct ttttccaatt tctttagctt ttaattcttt
     7081 tgaaacttct gccattttta caatcctcct ctgtcattac tatactataa aaacacgact
     7141 ttgtcattaa aaaaataatt atgacgacaa aatcatgaca aaaataaaaa agatattgat
     7201 ttttaacgac aaagtagtta tacttaaaat aagttgaacg acaaagtaag tgtaggagga
     7261 taaaaaatga tttgttcaaa caaagagaaa gtagaaacag ctttgatgat agattattta
     7321 aaaaaaaatg aaccaactga atggagaact atagaaacca taatctccag tacatacgca
     7381 ttaagtaatt tcaaaaaaga aaataaagac attatgaatc agaaataaca acagtaaata
     7441 cttaagggga gtgataagct tgaaaatttc agattttaat aaagatcata taggtaaagc
     7501 gattcaacta caagagacaa gatttaatgt cataacggat tgcatcatca cagaagtaga
     7561 agtaggcgaa attgttgtaa tgttttacat gcaagaaaat gatgacacag gatacaaggc
     7621 tataacatgg gaagatttaa aaaattatat cataaatggc aaagggaaga ggcattatag
     7681 gatttttgtg gcttggggga ttgttataaa aagaggtata acctcttttt ataactcttc
     7741 atttttgatt tttagatatg cattgtcaat tattttttct atcgcttctc tatcttcatc
     7801 ttccaatgca aatatctttt ttatcatagt ttttatttct tcactagttt ctaaatcttc
     7861 tgtaatgtca acgaacattt caccttctcc atgcaataac cattgttcat ttacattaaa
     7921 ttctctacaa atatcattta tggttctttg tggtatcttt ctatgccctg tttcatatga
     7981 agagatttgc gaaagagaca aacctaattt tttgccaaaa tctttttgtt ttaatttctt
     8041 tgaaagcctt atatctttaa gtcgttgtct tctttcatta tcttctaagt tttgtttttc
     8101 cataacgtca ccccttatga gtataattat aacatttaaa aatacgcttt gcaattacaa
     8161 attttaaaaa aatacgcaaa ataaaataaa aactcaaagc gtattgacat agtatgcaaa
     8221 gcgtaatata attgaatcac aggaaggagt gttagaatga atcaagaaaa agaaaaagcg
     8281 aatgatttgt tattaaaact tgaacttctt aaaaagataa acccacaaga gtttggatac
     8341 ataagcggaa gaatagacgt aaatattgag aagttaaaac aagaaaccga aagaaataaa
     8401 agcaaaaaaa taagatatag gaagggggct taaaaattga cttttaataa aaatcaaata
     8461 ggaacagcag ttcaattaca agatataagg tttgatacaa aagttgattg catcattaca
     8521 aaagtagaaa agaatgaaat actagtcatg tattatgaaa aagaaacaga ggaaatagca
     8581 tataaaactt tgactaaaga ggatctaata cttgatgatt acaaacttaa gttattatat
     8641 taaaaagtaa tttcggaagg gaggtgatta aatggatctt ttgaaagatg caagaaaaaa
     8701 agaatggttt tggctggaaa atgatttggt ggatagagag gatctaagtg tttacgaaaa
     8761 aatggcttat atggtaatgg caagatattc agatgatgaa agttcttgtt tcccaagttt
     8821 atcattaata tccaagaaaa tgggatgcgg aataacgact gtcaaaaaaa caataaatag
     8881 tttagaagaa aaaaatttaa taataaaagt acatcgcaag gttaaaaaca aaaaggaaaa
     8941 tgatacaaat gtttattaca ttatgagttt gaaaggtaag tcgtctggcg acataggtca
     9001 tgaggcgaca gacggtaggt cgccatacga ccaacaggta ggtcgcgagg cgactagtaa
     9061 aaagacttat aataaaaaga cttatataaa aagtaatacc actacacaaa aagaatctaa
     9121 aacttattta gacctatcat ttttagattt agatatagaa aaagtaaaac taactaaaga
     9181 tgaatatgac aaacttataa gcaagtttgg aaagaaatac atacatgata aaattgttag
     9241 tttagaaaac tatatcgtaa atggtaaagg gagtagatat aagagtcatt acagagcttt
     9301 gttgacttgg gggaatggag atgctagtaa aggtatagta ccagaagtta caaagactaa
     9361 gaatcctctt agtggattta aagaacttta aaaactaaat agtaaggagg tagataggat
     9421 gaatgaaaaa gaagagactg tttatttgaa tagttctaat gtgaagttat tcttaggtgt
     9481 tagggatatt aaggaatttg aggaattgat agaaaatgtc aatgagcgaa tttgtcaact
     9541 ccaagaatcg attaataaac ttgcccaatt taatattgag ttcgagatta agtcagatat
     9601 ttagttaagt ataaattagg aggattgttt atggcagata aaactgtaga acttactgaa
     9661 aaggatttgc actgcattgc aaggcatttg caaaatgaag tgttagagat agcatttaga
     9721 ggaaacagag aagcccctac atcttgtgaa gtttgcgatt acttcgaaga atgcaaggac
     9781 tatttcaccc atatagacac ttttataaaa ttaagtgaaa tgacaggtgt agatattttt
     9841 actaaataaa tttagtaaat taaaatttta gtattgtaat tgacgtgctt ttttaaaaag
     9901 aggacagttg ctattagcag tcattatttg aattttgcaa atgtaatttt tattacattc
     9961 gcactctaaa agttttgatc cagcgaatcc aggaaaagtt ttatattcac cagaaaattc
    10021 aactttatcg tcaataagag agcatttttc tcttaaggtt ttaattttgc acatattcat
    10081 caccacccta taattaattt gaacatactg ccaatatgct caaattaatt ataacatgca
    10141 aattatcatg gtgtagaatt aaagcatagg tcaattaaat ttagttataa aatcaatgaa
    10201 acagataaat aattaaatgt taaataaatt agggggttgt tcatgaaaga ggaaaagtta
    10261 gaacttactg aaaaagaatt gcattgtatt gcaagacatt tgcaaaatga agttatggag
    10321 atggtattta gagggaatag agaagccccc acatcatgtg aagtttgcga ttacctgcaa
    10381 gaatgtgagg gagattttac ctgtatgtat cattctttta ctaaattaag caaaataaca
    10441 ggtgtaaaag tgactgcatt taagtattgt tttccagatg aaaatcccaa agctactatc
    10501 taagaagttt ttttagaata ggacattctt cattagaaga agagtcgcaa atatgacttc
    10561 catcacatct agttgacatt aacttagatc caaaagtacc tggaggagct gaatattcgc
    10621 aagtaagaga aatggtttcg tttattacag gacattcttt gcttataact cgatattggc
    10681 tcatactctc accacttttc taattaattt aaacatattg gaagtatgtt taaattaatt
    10741 atagcatgta aattatcatg gtgtagaata gtttcaaatc aaaagaagca actaaatagt
    10801 taaagacagg aggtatgcta agtggaaaga aaaaaacatg aacttacaga tcaagatata
    10861 caaagactta ttgaaggatt aagaactgat ccaaaaataa gagaaatgat atttgaaagt
    10921 aaaggttgtg aaactagaac aagtacaaga agtaaatgcg aacaatttgt tgaagaaaaa
    10981 cttgcttata gaagttatgg atttgaaact ccaaaatact atgcaaaact tattgccgac
    11041 ataattgata ttttagttta caaaaatgtt ccaagagatc gtatttgtgg ggtattgtat
    11101 gacgtcgaaa gggtgattcc tttagttgtg acattttaat aataagctgg gggtgattaa
    11161 atgaattact ctttagaagc agagcaaaac attctaggaa gttttcttat aaatgactct
    11221 ataggttaca aaataaggga attaaaagaa aatgatttct actttgagtc acataaaatt
    11281 attttatctt gtatgaagag aataattgac tcaaataaac ctttagatct tttgctttta
    11341 aaaaatgaac tagaaaaaat agatagatta gtagacgttg gaggagttag ttacattacc
    11401 agtttaacaa ctatagtcat aactacttca aacatagatc actatataaa aatcataaaa
    11461 gagaagttac ttaaaagaca aatttcagag ttagcaaatg aattaattaa taattcaaat
    11521 tcagataaaa gtatagacga attgattgta gatataaacg atttaaaagc acttgtgacg
    11581 acgagcagta gtgtaaatga taattacatt gatgcgtcaa aaataaagcg tgaaaaaggc
    11641 gtacacaagt ccatagacac aggttttaat aaactaaaca atatgctaga tggatttaga
    11701 tatggaactc ttacaatttt gactggtaaa cctgcgtctg gtaaatcaac tatagttaac
    11761 cagtttatag cacaagcaat tacgaatgga gaaaaagcat ttttgtattc tggagaatta
    11821 ccctctttca tggctatgga ttggtttaga aaaactgtag ctaatgatta tcacataaaa
    11881 gaatataaaa gtgtctatgg aggaacatac acagatattc cagattatgc ctctgaactt
    11941 atttcagact ggataaaaga taaatttttt ctctatgacg aagatgcaat aagtgatgaa
    12001 gtaaatctac taaataccat agagcattta tatttaaaaa aaggggttag gttctttgtt
    12061 cttgacaatc taatgacaat aaaaactggc aaggcagata aatatgagag acaagagcag
    12121 atagtaagca acttaaaaaa ccttgctaaa aaatataact tagtaatagt tttagttgca
    12181 catcctcgta aaaatatggg tgacatgaag ccaactatgt atgatgtgtc tggagcaagt
    12241 gagattgtta attatgcaga ttatatttta tcaacataca gggttattga cgaagaagaa
    12301 gaaacagacg acacttatct attaatttta aaaaatagaa taacaggaaa acagaatata
    12361 agttttaaaa tgaatttttc agaaagaaga aaaagacttt acacgactag tgaggagtta
    12421 aacagagatt acaaatatga tgtaaataaa caatatgttc aagtagaaat tccagaagat
    12481 gtattttaaa aaaaggataa aaaataggaa attttattaa ataatgattt tataagaaaa
    12541 accaatattg aataatttaa aaattcaata ttgaaaaaat catttttcaa tattgaaaat
    12601 cggattttga aaaagtattg aatttcaata ctaaaaaaag tttgaaaata tagcatttgc
    12661 aacgattata taaattaatt tcctgacata atacgtacag gtttagatat gctagcaaaa
    12721 aacttaaaag tagtgtaagg ataaaaaggc tatataaatt tataaaagtt ttaaaagtgt
    12781 tattaaatta gagctaaagt ttattaatta atttcctcgt agaaaaatat tattttaaat
    12841 aaataaagga gtgattttta tgatagcatt agaaaaattt gcaggtggag ttctaaaaga
    12901 aaagtttaat acagagttac aaaaagtttt agataacatt gcagatccaa atacagattt
    12961 taaaaaaaca agaaaaatta gtttagaaat agtgtttaaa gcaaatgaag atagagatct
    13021 tgctgaagta gatataaaat ccaaatcaac tatagtagaa gcaaaggcta caacaactaa
    13081 agttctgata ggaaaagatt tagagacagg aagagttgaa gcgtctgaat ttaaaaatca
    13141 agtagcaggg cagttggtca tggatattcc agatagtgaa gaaactaatg aaacagaaga
    13201 aaatagtgct agtgtaattg atttcagaaa tgcaagttca aagtagttta agaaagtaag
    13261 taaaatataa atttaaataa aaaaggagaa taaaaaaatg ataagaaatg caatggaata
    13321 tttagttaag ttaggaaaaa gagaaacagt atgtgtagat gggtttaagt acacaacaga
    13381 tgcattagaa ataataaaag aaccaaaagc taatgaatta gaaataacta cattgagtgg
    13441 tttagtagat tatataaaaa gtggcataga tcataaggca gatgagttgt tattaataca
    13501 agttctaact ccaagaaaag tgcttttaaa atctcctttg agaaaaaata gagatagaga
    13561 gatatatatt gaatgtgttg cactactacc agagaaaaga tttgacagac ctttagactt
    13621 agatagtttt aacataatgc tacaatcagc atttataaaa aataatgata gagatacact
    13681 tttaaaaatt gcaggaaata tccaagaatc tacagtaaaa aatgttggag atgatggggt
    13741 ttctcaatct gtaacaataa aaacaggtgt cgctagtgta tcagaagcaa tagttccaaa
    13801 cagagtaaag ttaattcctt atcgtacttt ccaagaagta gagcaaccag aaagtgaatt
    13861 tattttcaga atgagtactg gaccagcagc aggattatat gaagcagatg gtggagcatg
    13921 gaaaaatcaa gcaatgttaa atattaaagc gtacttgcaa gaagaattaa aagattttaa
    13981 aaatgtaaat ataattgctt aattgaaaat ctaagaggac ttacaataag tcctcttagt
    14041 aaaaaaggag gggattagat gaaaatgatt tctcatatga acattttaga aagagcagaa
    14101 tttacagata aaatagctaa tgcaatcatg tctgcaaata aagaaattga aagaggctgt
    14161 gcctttaatg aagcagttga gattgtaaaa caaatggaat atagagaaaa taaatacaat
    14221 gaaggagata ttgcaatatg aataatgtag ttttagttgg aagattagca agagatccag
    14281 aattaagata tataccagag tatggtactc cagttgctac ttttgcattg gcagttgata
    14341 ggggttatac aaaaaaggat ggaactagag aagtcgattt tataccaatt gaagttatgg
    14401 gaggatccgc agaattttgt gctaattatc tcgcaaaagg aagaatggtt tcaattcaag
    14461 gccaaataag gatagaaaaa tatgaaaaag atggagagaa aaaaactttc acaaaagtaa
    14521 gaacaaaaat tgttaatgcg cttgatcaca agccaaagga tgaagaaaaa gaaattagtt
    14581 ttcaagtttt agatgatgaa gatattccat tttaaattgg atggtgatta tgtgagaagt
    14641 ttagaggaga gaaataaatt atttgaagat aatataaatc ttgtacattt tattataaat
    14701 agatatttca aatccttttt aagaaggtat ccatatttaa aagaagattt atttcaagaa
    14761 ggatatatag gattatataa aacaacatgt tgctttgatg aaagtaaagg aaaattttct
    14821 acaatagcat tttcgtatat atcaggacat ttgaaaagat ttacgactgg atatgttaag
    14881 aaacattata gaaatgatat agatagtttt gaaaagtgta tttatagaga taattatgga
    14941 gaagagataa gaatagaaga tagattggct ggcaatgaaa atgtagatat agaaaatgtt
    15001 cgtgttatta gatcttgtat aaaaagaagt gagataaaag atatacagaa aatagtttgt
    15061 ctaagagaaa aaggttatac tcaacaagaa ataagtaaag tgcttggaac tagccaaacg
    15121 tcaatatata ggagacttaa aaaattaaaa actgaaatta atatactagg taaataataa
    15181 atgatttaag tttattggag gtaaaatgaa aactaaaata attggtggta gggaaaatga
    15241 gtgtccaatt tgtaatggta atatatttaa gattgaaaca ttaattggaa tagtgtggca
    15301 atgtaaggat tgtggatgta tgtatcaaga tatgagttct aaagaaagta aaagggggta
    15361 ttaaagatga gttgtgttat aaagtgtgat ttttgtggtg aaacaatagc agaaattcta
    15421 aacataaagg tattcagaat tgatgatgaa ggtaatataa tgagtatcgg aaaagatgta
    15481 tgtggcaaat gttatgacag attatttgga agtaacttga ataagaagat gactaattat
    15541 gaaaagataa aaaatatgag caaaatagaa atggctgaat ttcttgcaaa tggcggaagt
    15601 ggatgtacta gttgtgctta tgattttcaa gaatgcttag gcggatgttt agaaggtaga
    15661 aaaaaatggc ttgaaagtga ggcgatgaaa taatgaaaaa gacgttaata atcgtaggag
    15721 tagttatttc aatattaatt atgtttgctt gcttgttctt agtaactaaa aataaagcaa
    15781 ttgatttaga agagcaggtc caatcagcaa ctgcatctgt agaggtgcaa gaaaaaagga
    15841 gaattgattt aattaagaac atagtagact gcgtaaaaga ctatgctaaa tatgaaaata
    15901 ggacattaaa agaagtaact gacatgagag taagtgcaaa tgatgaatct atcaaggaag
    15961 caaaattaaa tatacaagca gtagcagaga agtacccaga tttaaaagct aatgaaaatt
    16021 ataagcaact aatgagtgag ttgagtataa cagaaaatat gattgcacaa catagagaaa
    16081 attataatat gcaagttcgc aattacaata aatatataag aaaatttcca gcaaatttaa
    16141 ttctaaatag tatgggatat gaaaaaatag aatctaaata tactgaatat aaagcaaaag
    16201 aggatgcacc tcaaaattta tttgattaag aggtgcacat atgaaattta aaacttttga
    16261 aataactaag agagaatttt tagtgagcat aataattgct tgcatgatga ttgtgcttgg
    16321 atttttcatt gatgataaag ttcaatcaaa atttttagaa gataatgaaa gatattacaa
    16381 agcaactaaa ataaataatg acaaagatct ctttgattat gctatggaaa caagtgcggg
    16441 agattctttg ataagtggaa gatttaaaag tgtagattct gtatctatag aagaattaaa
    16501 aggggactat tggtttatag aaagaacgga agaaaaatac actatgcata ctagagtggt
    16561 taccactaaa gacagtaaag gccattcaca taaatatact gaaacatatt ggacttggga
    16621 ttaccatgat tcaatagaga aaaaatgtaa taaatttaat ttcatgggta aagagtttga
    16681 tgcgaaagta attactaatt tatctaaaga gagattggca ttaaagaaag atatatttaa
    16741 aaataataat cttaaaataa aaggtaatta tgcttacatt aataataaga gaaggtacta
    16801 ttatgaagtt ataccgaaag aatttaatgg taccatgtat gctaatttaa aaaataatac
    16861 tatagcgaat ccagctgata aaaacataaa agtttttaag ggtgagtcta tacagaatgt
    16921 tttagaaaat aataatttta atacagggtt ttttaaagta gtgttttgga tcatctggat
    16981 attaataact acaatcatta tatatgtatt ttattattta aaaaatgatt ggcttgaaga
    17041 taaataaaga tgtaagagga gaagatcagg tatgaaatgt aaggtgtacc atgctattaa
    17101 aaatataaaa gatgcaaatg agttaattga tagatttgaa acagaaaata tattctttga
    17161 attaaatgac atagataatg aaaatgtctt aaatggagaa atggatttaa ctgatataga
    17221 agatattgca tcattgcaat ttatattaga tggagattta tttatattta gtagttgtga
    17281 tgaaatgata tttatgaata aatacactca aaaagagttg ggacttcaat aaatgaatga
    17341 ggggagatac taaagatgga tttaaagtta attagcagta tacctaattg gaaggtagaa
    17401 gatcagttgg caaaaattga tgaagaggta actgagttta aaaatgctat agatacagga
    17461 ataactaaag atataattgc agaaggatta gatgtttgtc aaactatttt aacaatgttt
    17521 caagttttag aaatagaaaa ctatattact gaaggagtag agacacataa taagaaacta
    17581 aatggaagag gatgggatct caaagaaata ggagacgata tacttaattt aattaaaaat
    17641 tcaatgatta atgaattgat taatatgaac aagaaattaa aatgccttga tgaaaaagaa
    17701 tttaatgcaa aaatacaatt tttagatgta gataaactat ggaatttgtg catgccttta
    17761 gttaacttaa gtactataaa aggagttaat gaaagcattg atgaaatgaa aatgggagag
    17821 tttctaaaaa atattaatta aagtaaggag ataatataat atgttgatag aagtaactga
    17881 aaataataga gaattaaaag taggagatat tgttgaaatt aatggtggta ataagttata
    17941 tatgattctt tctttaaagg aaacaatagg tgggtatgtt attataaata tgcagaatgg
    18001 atatggttca tttggtgctt ataaaagttt aagtcaacta gaattagact tgaaggtaag
    18061 aggttataaa ttatatagtt ctgatgatta caagttgcaa ttagtgccta gagaataaga
    18121 tatatttcaa aaaagaaaaa aaaggagcat tacttcacgc tcctgcttgt caaaattcta
    18181 aaaccgtcat cacaacatca ttataacata attaggagtg tgaggtaatg caatctagca
    18241 aaaaagacaa tttgtttagt agtgcagaag gtaagttata taattataaa aaaataaaag
    18301 cggaaataga aaaaatagac attgatatgc agatgataaa aaacgactac agaggatgta
    18361 gtggagttga aattaaagaa aagacaagca agacatatga tattaaaagt gtaattgaaa
    18421 tagagactga agaaaaagaa aagaaaattg ccttaaaaga aaaagagaag agacataaag
    18481 agttgataat agaaaaaata gataatgcaa tgaaaatatt aagtgaagaa gaaaaaaaga
    18541 tagtacagta taaatacttt tcaagtagca ggacatcttg ggaatatgtt ggcagaatga
    18601 ttggtttttc agcgagtaag tgtaagcaaa tgaggtttga tattatagat aagataaaag
    18661 gattgctata aaaatgtccg atttgtgacg attttaaacc gatttatgtc tgttttgtgt
    18721 ctaaaaaatg tccgatttgt gaccaattta tgtctgtttt gtgtctttta atcatgataa
    18781 gattgtattg tgagaaaaat atatttaata aaaatggctt ggaatttacg ttctaagtct
    18841 tttttattgt ctagagaggt gttgaagatt aaacgtgata taacagaaga aatttcaagt
    18901 gctgcatatg taccagacaa tctaaagtat tacgacaatg taatgaaaga aactcctggt
    18961 atgtatggaa gagaatgttc tttagatttt ataaagaaaa agcaagaaaa actcttgaaa
    19021 ctaaaaaaga aggttaagaa aaattatgat tctaatatta ataagataga ctcatatcta
    19081 aaaattttag aggaaagtat aattgatgag tctgatcacg ttgagttggt tgtctttaaa
    19141 ttatatttgc agttagaaaa tgttgtaaaa gtaactgaaa ctgtaaatga cttaggattt
    19201 agaataaaga ccaatactta tgcaagagaa agaaaatata gtacaaatga tataacctct
    19261 ataatcacag atccttttgc taatgtagtt gaagacttaa aaatattggt gcaagatagg
    19321 caaagaaaaa attatcatgg taataaggag tgtttttaat gaatggaagc acatagtgga
    19381 caaaaagtta atccaataaa agatatagag gatgtattta gactcttgaa ttttctagag
    19441 gaatggaacg aaagaaatta cttattagct ctatttggaa tgtgtactgg gcttaggata
    19501 ggggatatat tagcacttaa agttgcagat gtaacagatg taaagttaga taaaaaaggg
    19561 aagaagataa gagtatctaa ggattggatt agagttatag aagaaaaaag agactatgat
    19621 agagaagttt tcttatctga tgtaataaag agtgctatag aaaactatac tcaagataaa
    19681 ccaggagaag agtttttatt taaaagtggc aagaggaaga aatttaatag gcctatccaa
    19741 acaaggcaag caggaagaat aataaaagga gcagcagaaa aggtaggaat aaaagaaaat
    19801 gttgcaacac actctttaag aaagacattt gcaagacata tttatgatga agaagaaaat
    19861 aagacttatg cattagaact tataaggaag attctaggac ataagacaat agaaatgact
    19921 agaagatata taggaataga taaggatgaa gaagtaaaag caataactaa atttactaat
    19981 aagttaaaga aaagaaaaag aaattaaaat atcttgtatt ttatctaaaa tttaatatgt
    20041 tacgtttttc aacaacagga cattattaga atgtaaagag cctcaataat agaatttcca
    20101 aagaatagga tagatttatt aaaatgtctg aaaatatata ttaaaggtca tttattcaag
    20161 ggatttatag ggatgtaaat atttaaatgt ttatttagaa gctgagtaaa gaggtgatta
    20221 attggatgtg aatgaagttg aaataatcga cagtagctat gaaaaaggta aagagttgac
    20281 agaagatcag tgcaatatgg taactctatt gattacaggt actacagtaa cagaaactgc
    20341 cagaattatt ggagtaaata gaaaaacaat ctataattgg atgaataaag agcatgttag
    20401 gaaagaaatg gacagacgga aacaagagct aacaaaccaa gggaatttaa tgatattaaa
    20461 ggatttagat agttatatca acaatatcaa ggaattagct agtgacaaga gcgataagag
    20521 ggttatgttg gcagccaatc agtatcttat aaatcgtata tatggaactc ctacaagtac
    20581 agttgtacaa gcagaggata atagtgttgg tatgggtatg gatgcaacag agatagaagc
    20641 agctatagct aagataagaa taagaacaag caagaagtaa ttagatatga aataaatcct
    20701 gcaagaatat gttttaatta tgtttgaaat caattaaagc aaaacatata ttatatatat
    20761 atttacttta ataattaaat catatattgt atttaacttt agttatagct aatttaataa
    20821 aatttagtga ggcttaataa ttaaagtttc atacagtgaa gtgatgtaga ttattatagg
    20881 atatgcgaac ctattggaaa tgctaggttc tttttaacta tgccgaaaat acatatttaa
    20941 ggaatagttg aaagataatt aataattcta atcaaaaaaa tatttcttgc aagaaaataa
    21001 ttcaaaatac tttaatatat ttattttttt tattaatagg gggtgtcctt ctattttgaa
    21061 aaaattattt tttatggaaa atgagttcta gaaattttct agcaaaattt aaaaaccaga
    21121 gatgtatttc aaagagggtg attttattag agaaaaattt acattgttta ttttattaat
    21181 tgcattactc actgagccta aaaaaataga agaagtaaga ggaagtaaat acgagtggat
    21241 aatctagaaa gaaatataca attaatattt agttatctag caaagacttt tataagaaat
    21301 ggagcatcta taaaagatgc tgaaaaagaa gcaaatgaga ttataaaagc caattcaaac
    21361 aatctttggg gagttaatgg tctcgcttat caacttggaa aaataaacct agaatttttc
    21421 tgtatgtatt ttatgcaaga cacatattta ccaaaagaag ataatgctgc tgctcctata
    21481 gctaaagttc atcatgagct atgggaagat atacaagaat ctataattgg agatggttca
    21541 cagcaattag gaagaattta tccaagaggg acaggtaaaa gtgcatttgg agatttagcg
    21601 acaactgtat ggtcgcattg ctataaacat aagacatata ctttgatttg ctctgacata
    21661 ggatctacag cagagaaatt tgtaaaagat ataaaaaatg cattacttga aaatgagtat
    21721 attaaaaaag catttggtgt ccttttaaat gataatgata gaaaatacat ttgtaatagt
    21781 actcaactag agctaacaaa taaaactttt attgaagcta tatcatcttc atcacccatg
    21841 agaggaagaa aatacaataa taaccgtcca gatcttatca tacttgatga ttatcagtca
    21901 gaagaaaatg ttagaacaga agatgctaga gagaaaaaat ttaaaagatt ttctgatgat
    21961 gtaaaatatg ctgctcaaaa accagtcata agaaatggta aaactataaa aagaggaaca
    22021 acttttatag cattaggaac cttacaacac aaagaatgtt tttatagtag actaaaaaat
    22081 ttaccaactt ggaagtttaa atgcgaaaaa ggagttttag ttgataatgt agatgaatta
    22141 tttaattctg gtttatggct taagttcaaa gagatcttat ttgattttaa aaatacaaat
    22201 catcttgaag atgctaaaga attttactgg gaaaatgaaa aagaaatgaa attccctata
    22261 ttatggccaa gtttttggga ttgtttagat atggctctaa gttattatga aaatccaaca
    22321 tcttttaagc aggaagtgca aaatgatgtc aattctatag gagaaaaatg gtttaaaact
    22381 gttagaacag aaactagaga agaaattgaa tcgcatactt ttaaaaagac aatgctgtta
    22441 tgtgatccag catctgcggg tgggtcgaaa catgactata gcgcttttct tgttggaagt
    22501 gaatcagaaa atggtttact atatggaaga ttggcagaac tagctaaaat aaatgctaga
    22561 actgattttg ataaatatat agaccatatg atttatttat taaaagtgta ttcagatata
    22621 acacatattt atatagaaaa aaatacattt aatggcgcag atgccaacca attagagttt
    22681 aaaataaaaa atgataatgt tcttagttat agagatatag aaatcattaa tgagcaacaa
    22741 aagaaaaata aagatgataa aatctctact ttaataccta ttttaaataa aggccaaatg
    22801 atttttgcag aagaagataa ggaatttata cagcaaatac ttgattttac aggacaaaaa
    22861 tactctctac atgatgatgc tccggatata tcatcggaat ttattaatcg aattaacaac
    22921 ataaaagttt ctgagacagt aaatatcttt gatagaaaat tattaggttt ttagttggga
    22981 ggtaatttat tggataatag atcaaagttt gaagaagttc aaaaaacttt aactaaaaaa
    23041 ggaaagaagt tcaatgttgg aataggaaaa gatgaaaaag gatattttgc atatactcat
    23101 agagcaagga gtaaaagtta tatttcaaag caagatattc caataaaagt tttaaaattt
    23161 atagagtcta caggatagga tgtgttgtta atggaattag atttagactt aataaaagac
    23221 atatatgacg attggaactc taataaatcc gaatatgata ctatgtataa gtattataaa
    23281 ggtgaaacgg atgctattag caactataaa atggttacca agagatctaa taataagata
    23341 aacactaatt tcttaaaaaa gtttattaat gaagaagtcg cttattctct tgcaaataag
    23401 attacctata caagtagatc tggtgatgag aagattataa atgatttaga atactatact
    23461 tgccattgga gtaaaaaaca tgactcagat ttattgaggt atgcactatt atttgggctt
    23521 tgctatgaac tttactatgt aaaagatgat gaaatacaag taaagataat caagccaaca
    23581 gatggctgtc attatgaaaa tgaaaatgga gaaatagtat gctttattag agagtttacc
    23641 aaaggtttcg aagatgacac gtatatagat gtatatgaca aagaatatat atatcatttt
    23701 gattcaaatt tcaaagaagt tgaaagtcct acagtaaaca atatatttga tggaaatgtt
    23761 ccaatatcaa tttgcaaaag aagtgaagaa ctaggaaaaa atacaatatt taatgatata
    23821 aaagggttac aagatgctta tgaaactaat ttaagtgata taagtaatga aattagtgat
    23881 tttagaaatg cttatttaac cttttctgga tgtaatatta aagaagatga tttgccaagg
    23941 atgaaagaac ttggaatact tcaagttaat ggagatggaa aaatagagtg gcttattaaa
    24001 gatatgaatg atacatttgt tcaaaataca ctatcttcaa taaaagaaaa tatgtatgaa
    24061 attacatctc atataaatca caatgaaaaa atgcaaagta atacatcaag tctagcagtt
    24121 atagcaagat taatcagtac agaatggatt tgtagccaaa ataatgatag tattgcagat
    24181 actttattta ataggtacaa attattgtgc atttggctta ataaaaaata tggttttgac
    24241 tatgattata aagatattaa ggctaaattt acccctaaga taccacgtga tgatttagtt
    24301 gtagcaaata tattaagcca acttggagat aaattatcta ctgaaactgg cttatctcaa
    24361 ctaagtttca ttgataatcc tcatgctgaa atagaaaaag ctaaaaaaga acaggaaata
    24421 gtttcagagg gagagatttt attagatgaa tcaaaagatt ataactaaat tgactgaaga
    24481 tatatataaa tctgctgaaa ataggacaaa accattttat aagtatcaaa gagaaaatag
    24541 ggataaactt ttattagaaa tagcaaagat attgctaaca cacgatatta ttaatgaata
    24601 cttaaatatt aataataaag ataagaaaaa tctaaggtca aaacttaata aaattataaa
    24661 tgagtattct aatagttatg atgaagaagt caaggatata gaaactattt tatctgattc
    24721 atctaagaat aaatattcaa atttgataat tttattagga gtaatactga agataaaaga
    24781 tgttagtaat ataaacaata aagttataaa taagatagtg aataataagg ttgatggaaa
    24841 acattggagc gatagactct ggaagaataa aaaaggtata gaagagtcct taaaaaaaga
    24901 aataaagagt tttctaaatg gaaaaacttc tgtaaataag atagaaaaag caataagaga
    24961 taaatattca acaggtgcag gacaaaccag cagactaata gaaaatgaag ttgccagatg
    25021 tcaaagtgaa gtaaatgagt attttttcaa ggttcaaggg attataaaag taatgtattg
    25081 tgcagattta gattctcgca catgtagtga ctgtagtatt tataatggag tgatatttta
    25141 tgtcaatgaa gataggcctt ctttgcctag acattgtcac tgtagatgcc aatatatacc
    25201 tatttaacat gtattaatta atttaatagg tgtttttatt atgtctaaaa aaatatgagg
    25261 tgaaatatga aagttgaaat attaggaaca gaatatctag tgatagagaa acttgaaagc
    25321 gaagatgtac tattaaaaga aagagctgga tattgcgatc actctgttaa agaaattgtt
    25381 gtagagaaaa taaattctga agaaggttca ttaaaagatt tgagtgtata taaaaatgaa
    25441 gttgctagac atgaaataat tcatgcattt cttagtgaaa gtggattgaa gagttgtagt
    25501 tcttgggcta caaacgaaga aatgattgac ttctttgcaa ttcaatttcc taaaatagta
    25561 aaggttatgg ataaaataga atgtttgaat taaattttca aaaaggagaa ctttatacat
    25621 gttaaaaaaa gaagtgttgg aattactaaa agaaatagaa gatgatgcag aaatagattc
    25681 attgttgcaa tcaacagatt tattcaaatc tgcatctgat aaaaaactta caatagaaga
    25741 gtttagagaa ttaattaata atgatgcaaa ctttaaagct attatagata gcgaaaaaaa
    25801 taaatatcac agcgaagcac ttgaaaactt taagaaaaaa gatatgcaga cacttataag
    25861 tgctgaagtt ctaaaaagaa ctggagcgaa tgaaacagaa gagcaaaaag cgataagaga
    25921 attaagagaa agtttaaata aattagagaa agaaaagcaa catgcagaaa aaatttctaa
    25981 atataaagat attttagttg aaaagaaaat acctacaaac ttaattgaat atctattatc
    26041 tgatgatgat gataaaacaa atgcaaatat agagatattt gaaaattcaa tgaagcaata
    26101 tgtacagtca aaagtagatg aaagaattaa agatggttca tacgttccac ctggcaaaga
    26161 tagtgcaggt tcgctatcag aaattagaaa acaaataaag caagggctaa atagtcttta
    26221 gtataaaaaa agaaaggatg attttataaa tggctaatgt attacaatat gtaacacttt
    26281 ttcaacaaga actagatatg caggtaacag ttggctcaac tactggatgg atggagaaaa
    26341 atgctggtca agttatatat aatggaggaa gagaggtaaa agtaccaaaa gtaacaatgg
    26401 atggcttagg tgattatgac agatctaaag gatatacgca aggcgcagtt acacttgagt
    26461 acgaaacaat gaaaatggag atggatagag gtaggacttt tatgttagat tcaatggatg
    26521 taaatgaaag taacttcgtt ataaatgcat cttctgtaat gggattattt caaaagcaac
    26581 atgttatacc agaaatagat gcatatagat actcaaagat attttctgaa acagctaaag
    26641 tagatgagcg tgtaaaatat tcatacgaag ctacagtaac tacaatatta agtgaattga
    26701 aaaatcaact tgcatcaata caagatttat ctggagatat acctcttatt attagtatgt
    26761 ctagcataat tgcaagcata ttagaacaat catctcaact tgatgatatt gaatttggcg
    26821 atggagatat aaaaagaatt gtaaagtcta tagacaatgt acctataata aaagttccat
    26881 caaaaagaat gaaaacagaa tacgtattta gagatggtaa aacatctgga caaacagagg
    26941 gtgggtttac tgaaggagca aaggctttag atataaactg gttaataact ccagcatctg
    27001 ctccaattgc agtatctaag actgataagc caagaatatt tactccagat caaaaccaaa
    27061 cagcagatgc ttatcaaata gactatagaa aatatcacga tttgtggata ttggatgaat
    27121 atttaaaact ttgtagagca tctgtgagac aagctaaagc tgcacaagaa taaggagttg
    27181 ttttaaatgt ttgcattgat aaaaaataac ataattaaag ttgttgaaga tgaaaattcg
    27241 agagataaat tgatttcaaa aggatttaaa atatattcag aagaagcaac taaagagaat
    27301 attgagaaaa aagaagaaaa agttaaatca acctctaatg atttatttaa tctatctaaa
    27361 ccagagctta tggaaattgc taaaggcaag aatataaagg gatattcaac taaaactaaa
    27421 gaggaattaa ttcaactact agatgagtac aatgcttgat aatgttaaat tagttttagg
    27481 aatagaaaat gatagatatg acaaattaat aatcctttat ataaataaaa taagtgagat
    27541 ggttttagaa tattgttcta ggcaggaact aagtatagca ctagaagggt ttgtagaaga
    27601 aaaagtaata tcaatattat cttcaagatt tgaagaactt aagaaaaata gtggacttga
    27661 taatctagaa aaaagcttaa aagctataac tagaggtgac actaagatag aatataatgc
    27721 tttaacatta caaactgctg aaaatagcat cagttcatca tcttttttaa ctgatactga
    27781 taaaaaattt ctaagcccat tttgtattgt tagtatgtac taaggggtga taaaatgact
    27841 gaagcagata tattagaaat gacatacctt gataaaatga ctataattag aaaattaaaa
    27901 ggctggaatg aagaaacatg ttctaatgat tttagtaatg ttgttatagc tgaaaatatc
    27961 ccatgtgcaa tttctagaaa agaagaagcc attgtaaatg gtgatattgg atctatagtt
    28021 gtaaatagaa agttattttg tagaccagaa gtagatataa aaataggtga tactataaat
    28081 acaacctata atcatggaga agaagctgta tttaaggttt ctaaggcaaa tatatatcca
    28141 agccatatgg aaatacctat tacagaagcg gagagaatat aatgggaata gataatttag
    28201 gagtttttga agcattagcc ttagatttaa agttaaatgc atcaaacttt aatagaaaaa
    28261 tcaagaagtt tacaaaagaa actgggcaag aactagcaga aaatataaga gatgaaactc
    28321 ctgtaaaaac tgaaaagtta aaaaagggat gggaagaaaa gcaaccagaa tatgatactt
    28381 atattgttaa aaatgaagtt gattatgcta ctttagtaaa taatggccat tcaactaaaa
    28441 atggtggcat tgtccctggt tcttttatgg ttgaaaaggg aattgaaaga acaaaagaaa
    28501 atatgacaag tgcaaaagca aatttatttg attggtaggt gattagttgc ttacaaacag
    28561 agacattata atagcagtta ataaaagaat cgaaaatgag tttagatata tagatatttt
    28621 tattaatgag gataatatac aaggctttga ggaggcttgt ttttttgtgc aacttttacc
    28681 tgtttctaat aatattgcaa ataaagtttt agatactaaa aagatattca tagatattga
    28741 atattaccaa aaggctagaa aaaataaatt aaatctatac gacatacaaa gtagattaga
    28801 aaaaatattt aacagaaaca ttagagttaa agatagaagt ttaatcattg agaatatatc
    28861 tccatcaatc gcaaaagatc ctgtaggata taaactaact tttctaatga ctgtatctta
    28921 ttttgaagaa gtctattttg aaaatgaaac atttgaaatt atggaaagtg tagatttaga
    28981 gataggaagt gattaaatgg gtttaccaga aattaatatt tcttttatcc aagcaggaaa
    29041 aacttttgtt aaacgctctg gtggtattgt agcgcttatt ttaaaagata caaaaaatat
    29101 agggttagtt gagataaaag aaatagagga tattccagaa aattattctc ccactaattt
    29161 agattatata aaaatggcta tgaagggaaa tgtatatcct ccaaataaag tattagttta
    29221 tacattagat acagatgaat caatagatga tgcactagat tttcttgaaa cttgtgagtt
    29281 taattatctt tgtatgccag atgagacaga acaagatttg cctaaaatca aaacgtggat
    29341 taagaaaatg agagaagata ataaaataaa ggttaaagct attactgcaa gtaattcagc
    29401 ggattatgaa ggtataataa actttacagc aactgatatt gaagttgaag gtaaaaaata
    29461 cacttcaaat gaatttctgc caagaatagc aggttttatt gcaggaactc catcaactca
    29521 atctgtaaca tatgcagaaa taccagaagt tactaatata cctaagctaa caagagctga
    29581 agcaaataca aggataaata atggagagct aattttaata aaagaaagtg gagctatagt
    29641 aatcgctaga ggagttacat cctttacaac aattactgat tcaaaaggtg atatattcaa
    29701 aaagattaag ttggttgata cactagatca aattcataac gatattaaaa aaataattgt
    29761 aaaaaattat ataggaaaaa ctcaaaacac ttatgataat aagtgtttac ttattgttgc
    29821 aatacaatta tatcttcaag aattagaaaa agatggttta attgatgaag gttcaactgt
    29881 agaaattaat ttagatgctc aaagagcatg gctaaaaaaa caaaatatag atatatctaa
    29941 tatggaagaa caacagataa aagaatacaa tactgacact cttgtatttt taaaagcaag
    30001 aattaaatta atagatgcaa tggaagatgt atacttagac atctctatgt agtttggagg
    30061 ttaaaatttg aaagctaaaa tagatgcaaa aagaataata tctggtactt ttggagagtg
    30121 ttggctagat aataagcaac ttacatcaac aaaagggatg gaagccaagg ttgatataga
    30181 taaagaagaa ataaaattat gtggtcaaat aatgaccaca tataaaatga ttggtgcaac
    30241 tggaaaagga tctataactc tttataagat aagtacctta tttggcgagt atgtcgaaaa
    30301 aatgttaaat gaaggtaaag aatttaaagt aactataata agtaaattgc aagacccaga
    30361 ttcatatggt tctgagagaa ttgcacttta tggatgtatg tttgacgact taactttggc
    30421 tgattgggaa gctgcgaaag ttggagaaat agaagtacca ttcacatttg aaggatttga
    30481 atacttagat aaaataaaag aaaaatagga ggatttttat tatatgaaca cagtagaaaa
    30541 attattaaat atggatgcag gtaaattaaa aatgccatct agcacatttg aattatattg
    30601 taaaaaacta gatgatactt tagagataga gtgtaaggct attgatccag aacgttttga
    30661 cgaaataaga atgaactcga tggatgttaa tagcggagat gtagaaaaca ttaacatata
    30721 tgaacttaaa gtaaatacaa tattagaatc atgcccaata tttagtaata tggaattagt
    30781 taaaaaattt agcgcaatta cacctaaaga attggttaaa aagctactac ttagtgggga
    30841 aatagataaa ttatatgagg aagttaataa agtaaatggc atagatgctg agggcgataa
    30901 aaaaagacaa aaagaaagag ctaaagatat aaaaaactaa ttaaaacgga tggagaagtt
    30961 aacttaatgt acttattatt taagtataaa ggtataatgc catccgtttc ttatcgcatg
    31021 ttaaaaggcg aaaaagaaat tactagtatc ttcatggatc aagaattgga tgagagaaaa
    31081 gaagaaatga aggctggcgc tggaaggtgg ttttaataca tgagtgatgg tacaagagtt
    31141 ttacaagcaa ttataagaat gagagatgaa gctagtgcaa ctttaagaag agttgcggca
    31201 aatacaagat ctctgcatga ctcaacttct aacgcctcta aggctttaaa taatgtccaa
    31261 aacaatttaa aaaatgttgg ttctaccgct ttaaaaacag gtagtgctat agctgctgtg
    31321 tctggtggga tactcgcttt aagtgcaaaa tctgcaatag aattcgaaag tgcttttgca
    31381 ggagttaaaa agactgtaaa tgcaagtgat gcagatatta atacgtttaa gaaaaatatc
    31441 atggatttat ctaaaaatat gccacaaacg gcagttgaga ttagtgctgt cggagaagca
    31501 gcaggacaat taggtattaa agtcggcaaa aatgcatcta atattacaaa ttttacaaaa
    31561 acaatggtta tgcttggaga tgcaacaaac atgtcgtctg atgaagcagc tacagcatta
    31621 gctagatttg caaacattgt tcaaatgcca caaaaaaact ttgatagact tggagcaacg
    31681 atagttcaac ttggaaataa cctagcaact acagaaagcg aaattgttga aatggctctt
    31741 agacttgctg gttctggaaa acaagttggg atgtctgagg cgcaaataat gtctttcgct
    31801 ggtgcattaa gttcagttgg tataaaagca gaggcaggag gttctgcttt ttctaaggtt
    31861 atgcaacaaa tgcaattatc atgcgaaatg ggaggaagta aattaaataa ctttgcaaaa
    31921 gttgctggca tgagtgcatc aaaatttaaa gagtcgtttc aaaaagatgc tgctggagca
    31981 attatatcat ttgtcaaagg acttggagat gcaaaaaaac atggaaatag tgctataaaa
    32041 atgttaaata agatgggaat aactgaaata ggcatgactg atgcattgct tagagcaagt
    32101 ggagcaggtg atctatttaa caacgctatt caacttggaa atgacgcttg gaaagagaat
    32161 gtagcactaa caaatgaagc aaatcaaaga tattcaacga cagaatcgca gcttaagatg
    32221 atgaaaaatc agattactca agtagcaata attataggga ataatttatt accttttata
    32281 agaagtgcag tccaagttgt atctaatatg gctaatgctt tctctaactt gcctccagcc
    32341 ttccaatcaa ctgtaacagg tatattgtta gctgttacaa catttggctt gttgttggct
    32401 actgtaggga ttgtaagtct tgttatttct aaagcagcag gcttattcgc tttctttata
    32461 cctataatat taagaacaaa agttgcattt agagcctttg taattggaat gaaaccatta
    32521 agtgcagtat ttttagctgt aatgggtcct atgggttgga ctatagcagc tgtaatagca
    32581 ttatcagtag cctttactgt tgcttatgct aaatctgaag tatttagaaa taaagttaat
    32641 tcattagtaa aatcatttac agaattcgca aagcctatag taaatttttg tatgcctgca
    32701 cttaaacaat taggagagaa atttaaagag ttgttatcag catttggtcc actatttgca
    32761 tctatcttaa acttaggaag tgtaataatg aaggtactag gacctgtttt aattggtgta
    32821 ataggttttc atatagcaaa attagttgta gttttatcta ctctagcaac tgcattcaaa
    32881 gctgtagttg atactataac taatgtatta aatggccttg catctatttt aaatggaata
    32941 tttgatttaa ttataggcat tgtcacaggc gatagtaaaa agatgatgga tggagttaaa
    33001 caaatatttg atggtggaac taaaattata aaatcaattt ggaaaggttt catagatttt
    33061 gtaactgctc ctatacaagc tatagccgat atagctgaca gcttatttaa agataaagct
    33121 aaatggctta cagataagtg gagagattta agaacattct tagaaaatcc aattgaagca
    33181 actgccaaaa tatctactaa aacaaatgca aatatcccag gaagtaaagc agcagatgca
    33241 gctggtaaat ctacaccaaa tacaggtgga ttaaaacaaa cagtagcaaa tctaaaaggt
    33301 tcatttagtg attttaaaac ttttattaag cctatgacag actctataag tcaaggagtt
    33361 gaatctggaa aaactaaatt tacagaactg caaaacaaaa tattaaatga tgttaaacct
    33421 gcattaaagg tcttagagga ttctggagta aattttaaag ataagtttaa ggagtcaata
    33481 acttcaataa aggataaatt taatgaatta aagaacatat tagctggaaa tattaaaaca
    33541 aatatagata atttagtaaa agcatttgaa cctttaaaac ctcatttaga taatttgaaa
    33601 gattgctttg ataaggttaa aaaagcaatt actgactttt ttactccagc caaacaggtt
    33661 ggagatacag taaataaagt aagtcagcca atggatagtg ttaaaaagtc tactgatgga
    33721 attaaacttt ctttccaaag tttaattcaa gcacttgctc ctgttaaagc ttggtttcaa
    33781 ggatttatgc ctttcctatc acaaatagga caacaacttt taccatctat aggaattgca
    33841 atagctggtg ttgttggcgg aataatttta gcatttacat gggttttaaa tacattaacc
    33901 gcaataataa caactgtttc tggaatcata aatggaatta tgtcttttat atctggaatt
    33961 attaatgtta ttatggggct tataacagga aatgctgatc aaactatgga tggacttaaa
    34021 caaatatttg atagtggagt aggtgtaata aaatcaattt ggaatggcct tgttgatcta
    34081 gttagtggcg tactagaacc tgtaatagat cttgctgatt ctcttttcaa agataaagtt
    34141 aaaggggtaa aagaagcttg gaataaactt aaagaatttt ttcataatcc aatcaaagga
    34201 atgatcgaga tagtaaaaag tggtcctagt ttacaaatag gaaaaggtgc ggatgctgct
    34261 ggcaaaagtc caagaaaagc atttggtata aatagagttc ctagaaatga ttatttaata
    34321 agagcacacg aaggcgagaa acttttaacc aaacaagaag ctaatcaata caaaaggaat
    34381 agttcaaatg gaagtttatt tcttgataaa ttggctgata caataattgt aagagaagag
    34441 gctgacatag ataaaattgt aagaaaaata aacaaaaaaa tatctcttgc aaaagttgga
    34501 ggcgttttgt aaattatcgt gctataattg tagtatataa tttacagggg gttatgttat
    34561 gaataaaaaa ataccagcat tagcaatatt aattgtatta tctttaagtt tagttggatg
    34621 cagcacaaat gattcaaaaa atggagatag taaacctaag caaaatacag aggaaattaa
    34681 aaatcataaa ataggcgaag ttgttgattt ggacggagca aaggtatctg ttaaaaatgt
    34741 tagaaaacta gaatcagata gtaatgtata tgcagttgag attgaaatca ccaatacagg
    34801 agatacagat cttgaaagtg atttccttca aaactacata ctaaaaacgg atgataatac
    34861 agttggaaat gcaataaata atcaagaatt aaatggagaa ttactatttg acacaataca
    34921 tccaggagat acattaaaag gtgagatagc atttgaactt acaaaagata caaagccaaa
    34981 aacaattgaa attgcttatg aaaatgtagg aaaatataca gtatttgatt tataataatt
    35041 atcttaaaag acacttattg aaataagtgt cttttatttt aggaggagtt taatggaaat
    35101 atggttaaga caagcaaata atacatttag gtttcctgtt attccaccct cttttgagat
    35161 aaatggaagt gcaacaataa atacatctaa tattttaagt gttggagata ttgcagtatt
    35221 tggagggtta gggcttaaaa ctatagaact atcctctttt ttccctaatc aagaatatag
    35281 tttctgtaat tataatggat ttccaaaacc atatgattgt gtaaatttaa tagaaagttg
    35341 gatgaaagaa ggatatatat taagatttat cattacagaa actaatataa actttgaatg
    35401 tataattgca gattttaatt atagagaaca agattattca agagatgttt attttacctt
    35461 aagtctaaaa gaatatagga ggatacaaat atccaaagta aacattaaca atgatgaaaa
    35521 attatcttct gaaaaggatg ttcaactaac aaaaggattt gatactaagc aaaaaacgca
    35581 taaggttgtt gaaggagata ctctttttaa gatagctaaa aaatattatg gcaatggaga
    35641 tttatgggag aagatttata aagcaaatga agatgaaatt aaagatccat ctgtaataaa
    35701 aaatggttgg gtattaataa ttccttaaaa tggtggtgat ttataattaa taaaatcaaa
    35761 atacaagttc atataaaaaa cggaaatata tataatgtaa ctgatttagt ggataaatgc
    35821 acttggtctg gtgattatag atcaccatca agaactttag aattttctat aattcaatct
    35881 gcaagtgata ttaattttag gcagatagat attccagtag caagcacagt ttgtttttat
    35941 gtagatgata aagaactttt tagaggaatg ataatagata gatctaaaga ctctaacaat
    36001 aataatatag attttacagc taaagatatg ggttttttac ttttacaaag cgaagtgtca
    36061 tacaatttta aagataaatt ggttgaagat attgcaaaac aagtattttt agaaaataaa
    36121 ctcccactag gtaatttacc taagactgga gttaaatata caaaaatgtt tattggcgta
    36181 actggttatg atactataat gagtgtttat acagaagcaa gcaaaacaac taaaaaaaag
    36241 tatatgatag agtctaatct tgataaattt aatgttattg agaagggtgt agttacttta
    36301 aatataacat ttgaagaagg ctttaatctt atcaatacaa atttttctga aagcatggag
    36361 aatgtaaaaa ataaagtact ggttgttgat cagtatggta ataagattag tgagaaaata
    36421 aatgactcta tatttaaaga cgttggagta attatgcaaa aggtaataca acaacaagaa
    36481 aacagtacta tagacataga tggagagttt aaaggaatcg aaaaaacttg ctctctaaaa
    36541 ggatatggtg atataacttg tgtaactggt agaggtgtaa aagtaaaaga ctcatataca
    36601 aaattaattg gtctttttta tatagataca gataagcata catggcaaaa tggagattat
    36661 caaattgaac tagagcttaa ttttgagaat ataatggatg aaaaatctgc tggtcaagat
    36721 gaacaaaaag aagatggttc agagtatact ggaggaacag aatactctgc tgaatttact
    36781 gcttattgtc ctagaaaatc tgaaggtgga gataaagatt gtagaggtaa aaaactagat
    36841 ccttctaaaa aaacatgtgc tgctcccatg gttggatcat atgagaaatc ttactataca
    36901 aaagatttct taaacaaaca tcctcttatg agatatggag atgaaataca attagttaca
    36961 ggtgtttcaa gtcgagatgg aacgtataaa gtaaatgata atggacctgc aataattata
    37021 gaaaaggatg gaacatatca tatagatatt ttatttggaa atgttgaaga agctaacaac
    37081 tttggaagaa gaaaaggaaa gattataatt ggtggttatt ctggaaacat gacagaaaaa
    37141 gcaaaaacag taatttcaga agcaagaaaa catcttggaa agccatacaa atggggcggg
    37201 aatggaccaa gtagctttga ttgttctgga ctaatggtat attgctttaa gaaggttaat
    37261 gttaatttac caagaacctc aagtcagcaa tctaaggtag gtaagaaagt agagaaaaat
    37321 aatttacagg caggagattt ggtatttttc cataatccag tcagccatgt tggactatac
    37381 gtaggtaatg gagaattttt acatgcgcca caaacaggag atgtagttaa aataagtaaa
    37441 ttaagtaata gaaaagattt taatacagct agaagagttt tgtaaaagag gtgatatcgt
    37501 ggcagatcca atcaatgaat ttataggaat aattaggcaa gaaggtaaat ttcataatga
    37561 accttctttt tttattggaa aaattaaaag taaattacca gatttaaaaa tagagataaa
    37621 taacatcata ttagaaaaag aagatatctt gatagatagt tggatgcttg atagacagat
    37681 agagttattt gatacagaaa caagtcaaga acataaacat gaaataaaaa atccctttat
    37741 agatactttt gaatctggaa acacagtaat aatgtttaaa ataggtgaaa aatttgctgt
    37801 tgtaagtaaa ttggtgagtc tagatgaata atactatatt ccctttcatg ggtgttccag
    37861 aagattatat ttctccagac aatgaagaat tacctatttt taaagaattt gcttgggatt
    37921 ttgataaaga tgaaaaggta attgaaaatg gagactttaa aatagttgaa agaaatgaag
    37981 caattaaagt atggatttac aaaacaataa aaacagataa gtatgtttat ttaatatatg
    38041 attgggatta tggaacagat ataaaaaatc taatagggca aaagtatact aaaggtctta
    38101 ctgaaagtga ggcaaaaagg tatatacaag aagctctatt aactaatcca tatatattag
    38161 aagtcaatgt aacaaatgca gaatttaaag atgataattt atctataagt ttaaatgtaa
    38221 aaacaattta tggagaggag gagattactt ttgtatagtg gccaatcatt tagtacctta
    38281 aataatagaa ctttaaataa cataaatctc cctctttata aaggccaagg atcttctctt
    38341 tataatatag tttctccaat aaattcagaa cttgcacaat tatatataga actttcttat
    38401 atacataaga gagtttttat tcaagataac tttgacgatt ttcttgatag aagagtcaat
    38461 gaatttggtg tatatagaaa gttaggcaca gaggcaatag gtgaggtaat atttgagggc
    38521 aaaataggaa ctaaaatacc aaatggaaca atagtatctc atagtgattt actatttgta
    38581 gttattaaag atgttacaat agatgaagat agtaagttaa atgtaagccc tatacaggct
    38641 ttagaggttg gcattaagta taatttatca gctaacacag aatttaaact tatagaagaa
    38701 ataactggag taactaaaat ttataatgaa cttgacctaa agggtggaac agaaatagaa
    38761 actgatgaag aattaaaaga aaggttctat aaaatacaaa aaaatcaagc gacaagtgga
    38821 aataaagcgc attaccagtc ttgggcttta gaggttgaag gtgtttataa tgcaaaagta
    38881 attcctcgtt gggatggtcc aggcacaatt aaaattctaa tttatggtca aaacaatcaa
    38941 tcagttgaca atgaagtatt gcaaagatgt actgaacata tagaagaaga aaaacctatt
    39001 ggacctactg ttacaattgt tacaccaagt acttttgata ttaatattag tgcaacttta
    39061 acacttgaaa atggatatga tattgaatct ataaaagttg tgtttttaga tattataaat
    39121 tcttatttaa tagaaaattc aagagagatt atttatataa aaataatgag tttgttagca
    39181 agcatagaag gtgttcatga tataaaaaat ctccttgtaa atgatgatgt taaaaacata
    39241 atagttgatg aagaaaaagt tcctgctgtt tcaagtgcta tatttgatat cgaggtgagt
    39301 taaattgaag ttaattgatt acttgccaag ttttgcaaac aatgaaattg acatacagat
    39361 ccaagaagca cttgaaaatg aacttctaac attaattgat gaaaaagatg atttactaga
    39421 tcagtttttt atagatacag caacttgggg gcttgacgat tgggaaagtc tcttaggtgt
    39481 aaaaataaat tacaagttag actttgatac tagaagaagt aatataaagg caaagattag
    39541 aggtagagga ataactacaa taaaagttat aaaggctata tcagaggtat acacaaagac
    39601 caatgttgat gtagaagtat ttagtaatct atttagtttt acacttagtt ttataacaaa
    39661 taattgtagt tataacacta ttttagagtt agataagaaa atagaagaaa taaaacctgc
    39721 acaccttgaa cataaatttg aaatgatttt atttaatgaa aatggacttt atgcaggagc
    39781 aatgaccagt acaggtgaga cagttactat atacccttat acacctaaaa atatagaatg
    39841 ttttggagaa ataatactag ctagtggaaa caataaaagt gctgaaaggg taacattata
    39901 ccctaaacag gaggtgatat aattggcaca agcacaatat tatacattac ttacggaaat
    39961 aggcaaagcg gctatagcaa acgctacagc acttggaact agagtggatt ttgcaaaaat
    40021 aaaagttggg gatggtggtg gaagtgcata tattccaaca gaaactcaaa cagaactcaa
    40081 aaatgtagtg tgggaaagca cattagagca tgttcaagca gatgaaaaaa atcctaattg
    40141 ggtagttatc caaaagacta taactggaga tactggaagc tttacaatca gagaggttgg
    40201 agtatttgac tctaaagacc aacttctcgc aatatctagc tatccagaaa cttataaacc
    40261 tgctccagat tcgggaacag taaaagaaat attaattaaa attatattag ctgtgtctaa
    40321 tacagcaagt ataaatttaa aaatagaccc aacagttgta ttagcaactt taaaagacat
    40381 acaggaccta gactctaaaa ttgatacaac taaaacagaa ttaacaagca acatagaaac
    40441 tgctaaaaca gagttaaata ctagaattga cacagaaaat gagaaacaaa atattaaaat
    40501 tgaccaatta atcgcaggtg gttctaatgt ggcatctact caaacaataa caattgacga
    40561 ttgggttgag gatgcagaaa atggattcaa agcaactgta acacatagtt tgttaacaca
    40621 gagaatagtt gtaaatatta tagatgctac tacaaaagaa aatgtagtta caaactttaa
    40681 aattatagat gataattcta tagaaattag aagtgaaaca aggtcagaat taaatgttta
    40741 tgtgataaat ggaaatgcag aaactcgttt tattaatgca actgtggatg ataacagagt
    40801 atctgaaatg actacttatt cgtctaagaa aatcgaagat agattggtta atatagaaga
    40861 aaaagtaaat ggaaatttat ctaatattgc aacaagtgta aatgagttga taacttattg
    40921 ttaaaggaga gtgagaaagt ggctacagaa tggaattttg attttaaggc agaagcacaa
    40981 ccaattactc taaaagcagg taaatataaa ttagagtgtt ggggagctca tggaagggtt
    41041 tggactggag attctccatc caatggagga tactcttatg gggaacttac attaaaaaaa
    41101 gaaactacat tatatgtata cacaggtgct gctggttcta gtaataaata tgaagaattt
    41161 acatttaatg gaggaggact tggtgttaat agtggaggag gtggagcaac tgatataaga
    41221 cttgtcaatg gagattggaa taatgaacaa ggtcttttgt ctaggataat tgttgctggt
    41281 ggtggtggag gcgcatttag taaaactcct gctggcaaag gtggtggttt taaaggcgga
    41341 aatagtacta atgatgataa cagttcgatg cttattgtac ctggtggtac acaatatgat
    41401 ggaggtagag gttatt
//
LOCUS       DAEPXF010000013        41400 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain HB27
            SAMN13376389-rid12479423.denovo.014, whole genome shotgun sequence.
ACCESSION   DAEPXF010000013 DAEPXF010000000
VERSION     DAEPXF010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13376389
            Sequence Read Archive: SRR10518432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 41400)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 41400)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001069624.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001069624.1
            Genome Coverage       :: 315x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/15/2021 01:54:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,165
            CDSs (total)                      :: 4,076
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 89
            rRNAs                             :: 10, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 10 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 19 of 69
            Pseudo Genes (incomplete)         :: 44 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 10 of 69
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41400
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13376389-rid12479423.denovo.014"
                     /strain="HB27"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="China: Hebei"
                     /collection_date="2017"
     gene            complement(75..479)
                     /locus_tag="KRL67_000643"
     CDS             complement(75..479)
                     /locus_tag="KRL67_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3139 domain-containing protein"
                     /protein_id="HBH1521548.1"
                     /translation="MKKYKVIFIVLIIGIISVTGFVVYKNYVKPWKDAETYIDKYMVK
                     QDISKDNIKSITKEKAKKASYEGILYKVYYKDDPRYEYQYFYSDNYYALYVNKVMLQI
                     YDMENNNKLLRSNEELKSVKYPPIYLKYKPDK"
     gene            complement(671..1858)
                     /locus_tag="KRL67_000644"
     CDS             complement(671..1858)
                     /locus_tag="KRL67_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosyltransferase"
                     /protein_id="HBH1521549.1"
                     /translation="MSKIVFFSIPAYGHTNPTIEVVRELVDRGNEVLYYSFNEFRDKI
                     EGAGAKFICCDKYLPELLPGDEKKIGKDFPRLIEMIVDTTISLDEKVCRELKDFNPDC
                     IVSDSLSFWGKLFAQKLNIPYVCSTTTFEFNEHTAKLMKQSFIEIIRMIFGIRRINKK
                     IKLLQRKGYEVKNFISIVSNDNDTDTIVYTSKEFQPMVETFSSKYSFVGPSVSKLIIE
                     PKERKRKLIYISLGTINNKNVSFYKNCINAFKDSDVDVIMSVGRSTDIESLGNIPNNF
                     KVKNSVEQITILQKTDVFVTHSGMNSVNESLYYGVPMVLFPQHSEQRMVANRVVHLGA
                     GIMLKEDKSESIKKAVFQVINDNEYKENATKLSKSFYNAGGSKKAADVILQIICNSRQ
                     VDI"
     gene            complement(1890..2315)
                     /locus_tag="KRL67_000645"
     CDS             complement(1890..2315)
                     /locus_tag="KRL67_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBH1521550.1"
                     /translation="MINENLRSLRKIHQYTQEELAEKLNVSRQSIAKWESGESIPDIG
                     SCIKLAKLYKVKLDDLVNHSEEKTGIIVPPKGKFFFGAVVVGERGQIVIPKEAREVFN
                     INAGDKLLVLGDEERGLGIVHQRDLINFIGHVGVVPKEE"
     gene            complement(2753..4702)
                     /locus_tag="KRL67_000646"
     CDS             complement(2753..4702)
                     /locus_tag="KRL67_000646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015394831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HBH1521551.1"
                     /translation="MERKYQNLCKPIKIGNVTFKNRMFSAPMGGTDITADCTIGSKST
                     AFYELRAKGGAGAVTISECMVHPETDGSHAYHLDLKIVDSLASFTYTADAIRRHGAIP
                     SVELSHSGQYSGTYLVDKDKKRGLSQWGVSPSVRPDGLEIKELTEEIIAEIVESYGNV
                     AALAKRAGFEMIMIHGGHGWLINQFLSPYFNKRTDKYGGSLENRVRFAKEVLDSVRKA
                     VGPGFPIEFRMSGSELFEGGYDLDYGIEIAKLLESRVDLLHVSAGTYQRGFAVTHPSM
                     FLEHGCNVYLAEEIKKHVNIPVATIGALNDPEMMEEIIASGKADVIYMARALLADHEL
                     PRKVMSNQDEKIVKCLRCFTCMAERATTSTRRCTVNPLIGRELDGTEVVPVLKSKKVL
                     IAGGGPGGLQAAITAVKRGHKVILCEKTNELGGILKGEQALPFKYEMYELGNTFGKIA
                     KDLGVEVRLNTTVTKEYVDNENVDALIIAVGSEPLVPPIEGLNGENVVIVNDYYLEKE
                     KVTDEVVVLGGGLAGCEAAIHLAQEGKTVHLVEMRAELAPDANIRHRPILLEEIERQG
                     IYVYTEHKGLSVTTEGVVCMNKSGNKINIPGTSVICALGQKPRREVVDTLLDCAPYVA
                     QIGDCVRASTITNAVYQGHHAALDI"
     gene            complement(4674..5972)
                     /locus_tag="KRL67_000647"
     CDS             complement(4674..5972)
                     /locus_tag="KRL67_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OFA family MFS transporter"
                     /protein_id="HBH1521552.1"
                     /translation="MNLTQKRWIILIASCFINLCIGSIYAWSVFASPMAEYLNSFAGT
                     TFTAASLAIVFTVTNSVGPITMITGGMINDKFGPKKVVFVGGLIFGIGMILAGFSKGL
                     PMLILSYGIITGLGVGMVYGCTISNSIKFFPDKSGLVGGITTAAYGFSSVIMPPVTNW
                     IINKYGVTSAFKSIGLIFLIIVCVSSFFIKKCPADFTPEGWVAPEKKGKVRVIINKNW
                     KEMLLTPIFYLMIIILTCGAFYGLMCVSLASSLATNMIGMTSSDAAIVVSILALFNTF
                     GRIVAGYISDKIGRINTLSGALVISIFGLLCLLFSREGDVATFYIGIIIIGICFGSFM
                     GIFPGFTVDKFGPKNNSVNYGIMFIGFAFAGYIGPAVMKNVYSVDGNYQRAFIIAIII
                     ALIGFILTFLYRYINKIQNLNIENLMKGDIKDGKKISKSM"
     gene            6224..6781
                     /locus_tag="KRL67_000648"
     CDS             6224..6781
                     /locus_tag="KRL67_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HBH1521553.1"
                     /translation="MRTLKYAILGLLNRNPMTGYDIVKEFNFQLAEFWNAKHSQIYPE
                     LKKLVNEKLIVYDIKISGDVLEKKLYTITKKGKDEFLKWLEKDEPMERTPKDIFRLRM
                     YFSNNLDIDTRLILLEHQLLQHQERITFLYSQMERYSEVPPLDSNDFGDYIVLDGAII
                     REQGAIKWLTNCINHHKNLDNTTLK"
     gene            7225..7974
                     /locus_tag="KRL67_000649"
     CDS             7225..7974
                     /locus_tag="KRL67_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440038.1"
                     /note="N-terminal region resembles flavodoxins. C-terminal
                     ferrodoxin region binds two 4Fe-4S clusters; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EFR1 family ferrodoxin"
                     /protein_id="HBH1521554.1"
                     /translation="MILYFSGTGNSRYVARKIAQELNDELISLNQLIKEEKTDELISV
                     DKPFIFVCPTYAWRLPIVVTDFIKKTKFLGSNRVYFVMTCGGDTAKSINYIQKLCKYK
                     EWQLKGMAEIKMPENYIALFSTTDKETSKQMIKEADKQIYRIISDIRNENEFETITPS
                     GLGGTIKSGIINTVFYKTIISAKGFHYTDKCIGCGKCVELCPLNNINLKNKKPVWKNN
                     CTHCMACICGCPTEAIEYKNKTQNRERYYLE"
     gene            complement(8135..8500)
                     /locus_tag="KRL67_000650"
     CDS             complement(8135..8500)
                     /locus_tag="KRL67_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HBH1521555.1"
                     /translation="MEKECQQRMQEITSKFKECRKAISAMGDETRQLILIALLESDFN
                     GIRVGEITEKTHLSRPAVSHHLKILKEAEIVNLRREGTKNYYYLDSKKSQWKSLTELI
                     NLIFVGIQHISENDIRKGD"
     gene            complement(8516..9292)
                     /locus_tag="KRL67_000651"
     CDS             complement(8516..9292)
                     /locus_tag="KRL67_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HBH1521556.1"
                     /translation="MKYQHIIEVDKELCIGCGLCKNDCPVNNIIIENKKSVIKRQDCL
                     MCGHCAAICPTKAITLTGFDEPPIELTNKPKLDSDELLMAIKSRRSIRKFKDKEVSSE
                     IVKQIIEAGRYTPSAKNSQDVSYIVLDNKKSIYENEAVKFFRKIKPIANIAIKYSKEV
                     EIDDNFFFKHAPIAIMIITKDKISGSLAASNMALMAESYGLGVLFSGYFSDVANNSSK
                     LKKLLGLKRSNHVLTTLVIGYPDVKYRRTAQKEVATVRYL"
     gene            complement(9685..11112)
                     /gene="ascB"
                     /locus_tag="KRL67_000652"
     CDS             complement(9685..11112)
                     /gene="ascB"
                     /locus_tag="KRL67_000652"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HBH1521557.1"
                     /translation="MSFKNDFLWGGATAANQCEGGYNEDERGLANVDVCPTGKDRTAV
                     ITGKLKMFDFDDEHYYPAKTGIDMYHNYKEDIKLFAEMGFKVYRMSIAWSRIFPTGDE
                     ETPNEKGLQFYENIFKECKKYKIEPLVTITHFDCPMHLVKKYGAWRNRKMIGFYEKLC
                     KVIFRRYKGLVKYWLTFNEINMILHAPFMGAGICFEEGEDVEKIKYQAAHHELVASAI
                     ATKIAHEVDSNNLIGCMFAAGSVYPYSCNPNDVWEATKLDRENYFFVDVQSKGKYPNY
                     ALKYMEQKGITPEMEPGDIELLNKYTVDFISFSYYNSRCVRTDENADDMAEGNIFTSA
                     KNPYLSYSQWGWPIDPLGLRITLNHVYDRYEKPLFIVENGLGAKDIADENGYVEDDYR
                     IDYLREHIKAMHDAVSIDGVDLLGYTTWAPIDLVSAGTGEMEKRYGFIYVDRDNSGNG
                     SLRRMKKKSFEWYKKVIASNGEDLY"
     gene            complement(11173..13101)
                     /locus_tag="KRL67_000653"
     CDS             complement(11173..13101)
                     /locus_tag="KRL67_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HBH1521558.1"
                     /translation="MGKYKQLAQEIVKNVGGKENISGLVHCITRLRFTLKDESIANDN
                     VLKNMEGIVTVMKSGGQYQVVIGNHVEAVYKDVVEIIGLDDSSTSSETKKSGNILDKG
                     IDIISGIFQPVLGIMAACGMLKGFNALFVAMGLYSDVSGGYMVINAAGDALFTFLPLF
                     LGYTSAKKFGLKPMLGLALGAAVCYPAIQGSSISAGTDVMYTLFKGTMFASPVYIDFF
                     GLPIVSIDYTGTVVPIIFIVYFASRCEKLFNRFVPDLVKFFFVPMLTLLITVPVALIV
                     IGPIATFGSTLISQIVISIRDFSPLLAGAIVGFTWQILVIFGMHWGFIPVYINNVMTL
                     GYDNVMMPFFACTFASSAVVLAIFFKTKDKKLKEMAIPNFISGIFGVTEPAIYGILLP
                     LKKPFIISCIASGIGGAFYGLFNLRKFITGGMGIFELPAMIEPDGGMGNLIVALSGIA
                     ISMVVAFVLTMILYKDEKVEESKKIRNKGKEEIKEVKSTKLEREIVTSPIKGEVLKLS
                     DIEDAAFASGVLGQGVAIIPSDGKVVAPVDGVVTTLFPSLHAIGILSDTGVEVLIHIG
                     LNTIQLEGRGFEACIKQGDRITKGQTILNFDVGAIKELGYSTVTPIVITNSSQFLDVV
                     ETESKNIELEDNLITVLF"
     gene            complement(13246..14088)
                     /locus_tag="KRL67_000654"
     CDS             complement(13246..14088)
                     /locus_tag="KRL67_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HBH1521559.1"
                     /translation="MIINKILNNNVVITLDDNDEEVIVMGKGIGYQKSKGNLIDKTKV
                     NKVFRISNKEISNKLQELLNNIPIEHMKLSSEIIEYAQIKLNKKLNESIYISLSDHTY
                     SAIQRMKEGINVKNAILWEIKRFYKEEFEIGMKALDIIENKTGIKLPEDEAGFIAFHI
                     VNAQLSEGHTLASDITKLIQEVLSIVRYHFRIEFHEESVFYYRFIMHLKFFAQRLLLD
                     SAHKGETDKELLSIIKSKYNKEFECVVKIKNFIKKQYNYILTDDELICLTIHLAKVVK
                     DSNM"
     gene            complement(14405..15805)
                     /locus_tag="KRL67_000655"
     CDS             complement(14405..15805)
                     /locus_tag="KRL67_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HBH1521560.1"
                     /translation="MKNKYEDVAQLIIDGLGGKNNIIDLTHCMTRLRFILKDEKKVNA
                     DKLKSIDKVVGVNSTSTQYQVIIGNEVGVVFNAIINEGINTTGDIKSDIKNENKKNSI
                     LNNIIDTITGCMTPMIPALTASGMIKVILSLCTTFNWLSSNSSTYRVLDIIGDVAFYF
                     MPIILAINAAKKFNVNISIAVIVVGVFLHPNFSAWISSGDPISFIGVPIQGVIYAASV
                     IPALLTVWMMSYIEKFIDKLTPSMLKTILNPTLVLLISAPIALIVIGPIGNFLGEGLA
                     SIINLLQGRLGFIMVCLLAAAMPFIVMTGMHHALTPIFISAFATTGQESLILISQVCA
                     NLAQGGATLAVALRSKDSKMKQLASAAWISAIMGITEPALYGVTLKLKKPVMLASIGA
                     GIAGLFAGITHVTLYVPQNSIMAVLGLSGEKGISNIVNGLIVIFIALVAPFIMTYVFG
                     VEKNVKESTDNVVDRV"
     gene            complement(15844..16515)
                     /gene="pgmB"
                     /locus_tag="KRL67_000656"
     CDS             complement(15844..16515)
                     /gene="pgmB"
                     /locus_tag="KRL67_000656"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HBH1521561.1"
                     /translation="MKAVLFDLDGVITDTAKYHFEAWYEIAKSLDIEIDETFNEKLKG
                     ISREESLDKILLSANKVDSYTKEEKLKVCEEKNNIYIKLINSLTYKDILPGINGLIKE
                     LKKNNVKLGIASASKNAPRILESLGIKDEFDCIVNPSLLERGKPYPDIFIEGSKMLNL
                     KQYDCVGIEDSCSGIKAINAANMTSIGVGKKEVLGKCDHIVEDTSLLNYNTLLQAWSK
                     KINLA"
     gene            complement(16517..18913)
                     /locus_tag="KRL67_000657"
     CDS             complement(16517..18913)
                     /locus_tag="KRL67_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HBH1521562.1"
                     /translation="MSLIFTTKDQLIEQGFSKDTIKSNETLFTLANGHLGVRGNIEES
                     DFNNEYIDNMGTYVNGFYEESPITYGESAYGYAKQNQTICKLPNAHIVNFSIEGDCFD
                     LSKGITSEHTRILDLNEGILKRSFIWENSRGKKVKVYIERLVSQDIEELMMISYTITP
                     LNFNGKIKFKNKMENSISMEKFNCEKVDDPRVASINKKQFKIDIKKYNNKYFMNLVTE
                     RSKLGLWCGFDSYITCGNITNETYNVSDECIEREIEVYAKKDERLNLEIVVGYSRVYS
                     DLNLNEERLKSLECLESLKCLESEAHLKSLELVLREACDIGFDKVKNIQREKMNKFWT
                     NSDVEIEGDKTLQLGIKTNLFHIYQSAGRDGKTNISAKGLTGDGYEGHYFWDTEMYIL
                     PFFIYTQPSIARALLDYRYSILDASRERARELGINKGCLYSWRTINGNECSAYYPAGT
                     AQFHINADIAYGIRTYFEATNDEEFMSQNGLEILIETARFWVEFGDYIESKNNKFCIN
                     GVTGPDEYTAIVNNNYYTNLMAKYNLIYAVQMVDKFKHNLFSNEVFRKVKLQEYEVKN
                     WINAAQNMYLPYDEEKKISKQDDSFLEKSVWNFKNTPKEKYPLLLNYHPLTIYRYQVN
                     KQADTVLSALLFPDEFSLEQKKRDFEYYEKITTHDSSLSRSIFGMMASEIGNHEKAYN
                     YFMDTALMDINNLQKNTSDGIHAANMGGTWMSLVYGFAGMKIKNDKLSFEPRIPKHWK
                     NIKFNILFRDNLVSVNIEEYKSYYKLIKGKNISILHDNKEIKLSKNVIEVNNCFKRSN
                     "
     gene            complement(18939..20387)
                     /locus_tag="KRL67_000658"
     CDS             complement(18939..20387)
                     /locus_tag="KRL67_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HBH1521563.1"
                     /translation="MKLRKDFLWGGAISANQSEGFYLSGGKGLSQFDKLPLNDKRLKD
                     VCLDEDNILNENNQYYPSHIGIKFCENYKEDIAMLAEIGFKCFRFSISWSRIFPTGEE
                     SEPNEKGLQLYDNIIKELKKYNIEPLITISHFDIPYSLVEKYSGWANRKVVNLYVKYA
                     EILMKRYKDDVKYWIPFNEMNMIMHIPYIGAGLTFIADENQLQKKYQAAHHQLLANAK
                     TIEIGRCINKEFKFGCMLAAGKTYPYTCKPEDVFATLEHERHNLFFSDVQVRGVYPSF
                     MNYYLKSNKIKLIIKEEDLSTLKNNTVDFVSFSYYSSACSSARAEGLEKSKANGPETI
                     KNPYLPKSNSIWQNDPVGLRITMNQLYERYQLPLFIVENGLGTQDIVTEDGKIIDDYR
                     IDYMKEHIKNMILGVNEDGIELLGYLMWGCIDVTSVSEGKMTKRYGIIHVDSDDYGRG
                     SFKRTKKQSAFWYKKVIETDGEILFNDMETHA"
     gene            complement(20792..21679)
                     /locus_tag="KRL67_000659"
     CDS             complement(20792..21679)
                     /locus_tag="KRL67_000659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HBH1521564.1"
                     /translation="MAIYLEMPKLIHEFPFRSLSNEGKVLTTPHWHKEIEILLITEGV
                     VNLFINDKPMQLKKGEIVIIKGGDIHYILPSPGSERLVFQFDISFFNDLILLNEEKES
                     LINLFSSIKKCSRDWSAKISKSVFDILIDIYNEDLYKIEGYSYAIKGKMYNLIPILYR
                     QIPREIEENTVEYEINSKEILERLNNIFKYVEKNYKQPIKLEDVSYEVGFSVYYFTKF
                     FKKNTGKTFITFLNEYRLEKAKWILLNNNISIVDLVEEVGFGSTKTFYRVFKEKIGVS
                     PLEFKKRYNSLEKKGGVSK"
     gene            complement(21778..22227)
                     /locus_tag="KRL67_000660"
     CDS             complement(21778..22227)
                     /locus_tag="KRL67_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBH1521565.1"
                     /translation="MEIREILTEESIILDLKSTTKEDIIQELVDKLYDTGSITLKSKF
                     IKALKRREKIGSTSIGYQMAMPHGKTKYVKKASVVIGKSEKGVDWPSIDGLPVKVIIM
                     FAIPQSAGNEDMILISKIAYILGGKNISERLLNAHTKKEILDIFYEV"
     gene            complement(22217..24124)
                     /locus_tag="KRL67_000661"
     CDS             complement(22217..24124)
                     /locus_tag="KRL67_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HBH1521566.1"
                     /translation="MLVSRQIEFLKLILSENKYRPIKYFKDKLNVSDKTLQKDLKMIE
                     KYLETFNIKIDIKRGYGILIEDLAKKNIDLINSLNIQSKENKSLSVEQRRMEILKYLL
                     LNSNNITSINQLADKYYVSKTSIVNDFKYIEAWIKEYNLKLNKTLEGTKIIGKEVDIR
                     KSIAKMMDDLLDENYEQHDISELTRLDSVTFSALINLFDIDSIIFVETIITELEYNLG
                     YTINQPYYINLITHILICLKRIEEGNQIESQEEREVSVDNLDELVYKNVTTLIDKIEN
                     RYRVKITKDEIMYIYIFLVSSGFSSESNSSYNDESDRLSESEKVSSIMIENMSQFLNI
                     NLKNDDLLQKSLASHVRPMLNRLRYDIQIKNPLLGEIQERFSDVLGLCMMTINIMTEY
                     DNLKNISIDEVSYLATYFQAAIERNMSSKRVIVVCHTGYGTSQLLAARLKREFPEWTI
                     VDIVPMHQLSKRNLDDVDFIISTVNLDLKDKLHIVVSVLLMESDINNIKNALLSKPKK
                     SMNLDTNLLGMYLEDNIYFNFDVVQMESLIGVNLGSKYESISLGKDLNIYIHKQSKLN
                     KGIMNINNVNRTIDIYLEISNQSFLKNILMEISNLYRQKNYINYIIDCKNKEDIKILL
                     DNNLLEKTNGN"
     gene            complement(24407..26302)
                     /locus_tag="KRL67_000662"
     CDS             complement(24407..26302)
                     /locus_tag="KRL67_000662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBH1521567.1"
                     /translation="MIGKILRSDCIVLDKQLQTKKDVIDYMANHLYHGNYINNKEKFI
                     ADINKREKESSTNMGNLIAIPHAKSNVVNQPVMMYLRTDREITDNDGQKLRSFFMIAV
                     PESGSDEHLKIISKLATYLMEENFLKHLLSAETPYEILSIFNNKAAESGDFSNNQNKG
                     LIVGITGCPTGIAHTFMAKKALEEAGQELGYEVKIETHGAIGVENKLTKEDIKRAEYV
                     IIACDKEVQLSRFIGKKVLQKKVVDAKNVLLAKDLIINAENGMAKEIKPGGKTLEDSK
                     EILQGSEKKGVYKHLMSGVSEMIPFVVIGGIGIAIAFMFGIFASDPTHETYNPIAGFF
                     GQLGGDSAFKLFIPILAGFIAKSIAGRQGFAPAMIAGFMAVIGGSGFIGGMVAGFATG
                     YIALGVMKITENLPDVLKGVNAVLICPLLIAFTSGAIMFFVVNTPASWLNEALQSWLQ
                     SMSGTNKVLLGAILAGMMASDMGGPINKTASAFGLAMFSSNIFEPSAALMVGGMVPPL
                     AISLAMLIFKNKFTKEERKSSLPTAIMGASFITEGAIPFAAADPLRMIPANIIGSCIG
                     GAICMSLDITLMAPHGGIFVIPFACSAPVAYIACILIGTVITALIIGVTKPTIRSENS
                     EVELLGA"
     gene            complement(26338..27216)
                     /locus_tag="KRL67_000663"
     CDS             complement(26338..27216)
                     /locus_tag="KRL67_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HBH1521568.1"
                     /translation="MLINMKEMLKVAQENQFAVPAFNIGSGQILKAVVQSANEKNAPV
                     ILAIHPNELSFLGDSFVASCIEEANKSKVPMVIHLDHGENKEQILRAIRCGFTSVMID
                     GSHLPYEENVAISREIVEIAKGLNVSVEGELGTIGTTGTSSEGGTDEIIYTDSKLAKD
                     FVEKTGVDTLAIAIGTAHGIYPKGFKPELKLDLLKEIREVVDIPLVLHGGSSNPDEEI
                     AQAVKLGVCKINISSDVKSAYYKKCRELLEQNPSLYEPDTIYPPCIKVAREVIEFKMN
                     LFNAIDKLKCFYKNKN"
     gene            complement(27921..29354)
                     /locus_tag="KRL67_000664"
     CDS             complement(27921..29354)
                     /locus_tag="KRL67_000664"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003243232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HBH1521569.1"
                     /translation="MKSNQEFLWGGAVAAHQVEGGYNKGGKGISIADVMTAGTHTISR
                     KITDGIIEGLNYPNHEAINFYENYKEDIRLFAEMGYKCFRTSIAWTRIFPNGDESIPN
                     EDGLKFYDDLFDELLKYNIEPVITLSHFEMPYHLVKNYGGWRNRKLIDFFVNFCEVVM
                     SRYKDKVKYWMTFNEINNQSITTNPIYAFTNSGIIYEEQEDKEEVMYQAVHYEFVASA
                     KVVKIGHEINPEFKIGCMVAAMPSYPYSCNPEDMIKFVESNREQLMFTDVHVRGHYPR
                     NTLKLWERKNYNLDITEEDKKILKEGIVDFIGCSYYLTTVVTADKTMKTTGNNSAGKA
                     DVVENPYLKTSDWGWNIDPVGLRFYLNQLYDKYELPIFIVENGFGAEDILKSDNTVDD
                     DYRIEYLANHIREMKNAIEIDGVDVIGYTVWGCIDPVSFTTGEMKKRYGFIYVDKNND
                     GSGTLKRYKKKSFYWYKKVIKFNGEIL"
     gene            complement(29377..31218)
                     /locus_tag="KRL67_000665"
     CDS             complement(29377..31218)
                     /locus_tag="KRL67_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HBH1521570.1"
                     /translation="MKFERESEEIIKYVGGKENIISLVHCATRLRFKLKDTSKTNKEE
                     LLKMKDVLSVVNSGGQYQVVIGNKVTDYFETIIKKAVLQGQNTEEQGEKVSIVSKIFE
                     SISGAFSPLIPALAGAGMIKALLTILTTMKWMSDTSSTYAILSAAGNAVFYFLPIFLG
                     ITLAIKLGANPYVGGVIGAALLDPNFTALIGAKGGANFFGMPVTAIDYSFGVFPIFVA
                     IFIYYYVDKLLKKIILKDLQLFLVPMISLLVMVPLTVILFGPIATNVGDAISNIFMGL
                     FEFNRVIAGAAIGIAMPFLAVLGLHWGFTPITLQNLATMGGDPIEGAAVAAVFAQIGI
                     AIGLFMKVRKSKKNSELKVLAGSTAVTGILAGVTEPIIYGIILRYRRMIPVLAIAGGI
                     GGGICGVFGVTCNAYVFHNIFSMPVYTPFLGYILGIGTALVLGAVLSYFFGLSKEECE
                     EGNEEVAFDEDDGIEEILSPIEGLQIELSEVEDEVFASGTIGNGMAIIPSKGEVVSPV
                     NGTITTIFPTKHAIGITSDKGVEILIHVGLNTTMLDGKYYTTHMNAGDKVKKGDKIVS
                     FDKDKIEGEGYSIVTPIILTNTNDLADVIVKENKHIKIGTPVIKVVY"
     gene            complement(31344..32177)
                     /locus_tag="KRL67_000666"
     CDS             complement(31344..32177)
                     /locus_tag="KRL67_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HBH1521571.1"
                     /translation="MVIKKVFNNNVVFARDNSGQEVILMGKGIGFNKFTKDLVEIDKI
                     EKKFIFNSEESLSELHSLLDRIPLKDVELVSEIIQMGREELSDNLNDSILITLSEHIS
                     YMLKRMDEGLVFQSPLQWEIRQIYPNEYNFSKKAVEYLRKKLDKDIPDSEIAFITIHF
                     ANAQLETKDMQETLLLTKIIDNVLDIIKYHYGIEINEYSFDYTRFISHLRCFIKRQLS
                     GENTNVDTSLLDIIKMKYKSDYNCAKKVKNFLEKTYKWKISENELLYLTLHLNRLSNI
                     K"
     gene            complement(33222..33434)
                     /locus_tag="KRL67_000667"
     CDS             complement(33222..33434)
                     /locus_tag="KRL67_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBH1521572.1"
                     /translation="MAILMKTKIKEYREKLLMTQNELAKLVGVRRETIVHLENGKYNP
                     SLKLAMDIVKVFDTTVENLFEFIEEE"
     gene            complement(33435..33851)
                     /locus_tag="KRL67_000668"
     CDS             complement(33435..33851)
                     /locus_tag="KRL67_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH1521573.1"
                     /translation="MSKSETGVKSKLILELLVITYFFIAVLATKYIFKGPSTASLPGL
                     SWLIVFGCYSIYNKKYNDLVDESSEFILSKVNKIAIKIMIYSIVIISIFFATPYTSNI
                     NISNIDTGIILIAVLFIQSLSKLVLYMYFDKRGICN"
     gene            complement(33919..34332)
                     /locus_tag="KRL67_000669"
     CDS             complement(33919..34332)
                     /locus_tag="KRL67_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH1521574.1"
                     /translation="MSKSELGVKTNLVSELLMMSYFFIDSLLEKFIFKGGNIWITFII
                     LFAFWACDFMYNKKYNDLVDEASKLMLLKVDHIASKIMLYSIFLIAVFFSANYTRGLD
                     ISNLDIGVILLAILFIQSLSKLILYIYFDKKGICN"
     gene            complement(35177..35806)
                     /locus_tag="KRL67_000670"
     CDS             complement(35177..35806)
                     /locus_tag="KRL67_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine amidotransferase"
                     /protein_id="HBH1521575.1"
                     /translation="MKNRKVYMYVFDTMSDWEVAYLTAELNTGRYFKKGLKPLKVITV
                     GIDKNPIITMGGLKVLPEITVDEFNMESKDLLILPGGNTWLSMNHETILEKAKEALIQ
                     DSIVAAICGATLGLAKKGLLDFRNHTSNDLEFLKMVVPNYSGEKYYKMESVVNDENLV
                     TAPGTAPLEFTFQVLKILDVFESESLNSWLNLYKTHDSKYFFELMNCNK"
     gene            complement(35885..36847)
                     /locus_tag="KRL67_000671"
     CDS             complement(35885..36847)
                     /locus_tag="KRL67_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017561613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YafY family transcriptional regulator"
                     /protein_id="HBH1521576.1"
                     /translation="MAKIDNMMSILWMLSSDKKITAKQIAEKLEINIRTVYRYIDVLS
                     ASGVPIISDTGHNGGYSLLNNFTKAPLFFDIEEQKALLHAALFAKKEGYFLDEALDKA
                     ILKLKMYSNQKQEDILTNHLIALEVIKPIGKLSIESVLKKLENSILKELSVEINYHTG
                     RKAKSKYRIINPYGIVHWNNNWYVVAFCNMRNEIRSFRVDRISEIIETSNTFNRPTEF
                     SASEFFIKGIIPEIKNEQGIIQLVVEGSIDSLDSLSQHWFLGHYLKERTPNKIIFMLE
                     EEVICKYIPNILLPYRKTIQVIQPLSLRKKMIEDLLELIDYYQI"
     gene            complement(37608..41246)
                     /locus_tag="KRL67_000672"
     CDS             complement(37608..41246)
                     /locus_tag="KRL67_000672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH1521577.1"
                     /translation="MQQKCKKKALSMILSISMGLGSVASFNASNTFADTIEESTNAGN
                     VEGDTNAGNAEGDANAGDAEGDTNAGNAEGDTNAGNAEGDANAGNAEGDTNAGNAEGD
                     TNAGNAEGDTNAGNAEGDINAGDAEGDTNAGDAEEDVNEVIVEEDAIAEESMSAGDFK
                     ISGGEIDVDFKFASGVLTILSGKQMTIQNNNPNTPTNQRIVVPKDVKAELIFAGVNIK
                     ATCPFTLTPDKDGGAYAHIILQDDSKNILTSSNIYYPGMRAGKSTTLIIDDSVVNRDI
                     EGEFIVPENGKIPRDVTLENGDTLKKGDRLTLLDSKNRGSLTVVGNTHSSGIGGGDTE
                     SGGNIIINGGIINATALGGSQSAGYGAGIGGGGNGTGGDITINGGTVTARGGYHGAGI
                     GGGYYLSLKGVLSPAVVDPNTGQGYSGNITINGGLSYSYGGAHGSAFGDGCVNSGTNR
                     GYRVLVTGGTILPYMTGSGSWIFDLSATTGEVIVTGGSLKATTFKSFNGAVAYGDLEK
                     KTKVFMNKISLTYLGQDKVNTTLVDDFKMLINGKIYPYGAPSYTDAQGILYLWFSDQF
                     KGSEVSVDLSVIDKITGEILKPDDFYIKDIGSGSQFLKQYEEFTIDDTPPYLINKRYD
                     GLPFEEDVEKGFLDWILKDGGVPVTTPKGEKLTDKKFMTISSQRLNDETMEPDTNLGS
                     NDLTKQIDAGKYQLSITSTQFSSIYPFKDAYWGHRAYYKAEIIPADAETSLVVEESKV
                     KTDVFKPTDAFTLNATVSPDKKEGPDCASPRGYVQFYINGKEYKDPVELKTHDKGSES
                     HNYSTASIEWRPLDSNHHVLTTEQTITVKYIGDDINYIEGSEDEKKLKLDMTNVDVDG
                     DGKPDINIDTDGDGRPDINIDTSGDWKPDINIDTDNTGEWKPSTEGGNGDGIWKPDKN
                     IDTNGDGNPDTDYNRPAIDTDNDGVDDYWKPDKNVDIGSGGYDTGNPDPNKPDSGNGG
                     NSKPDSGNGGNSKPDSGNGGNSKPDSGNGGNSKPDPDNGGNGKPDPDSGGNSKPDSGN
                     GGNSKPDPDNGGNSKPDPDNGGNGKPDPDNGGNSKPDPDNGGNSKPDIDIDTDGDGKP
                     DIDIDTDGDGKPDINIDTDGDGKPDINIDIDGDGKPDINIDIDGDGKPDVNIDIDGDG
                     KPDINIDTDGDGKPDINIDTDESLDSNVGQNVQTGDQSNIMLDLALMFISLFFLIKNL
                     TNKYLRRK"
BASE COUNT        13353 a         7464 c         4360 g        16223 t
ORIGIN      
        1 atttaatttt atttaaacac tctagtgata tttttaaata aattttaata aaaatccttc
       61 tattttgtca taaatcattt atctggctta tatttaagat atattggtgg atactttaca
      121 ctttttaatt cctcattact tcttaaaagt ttattattat tttccatatc ataaatttgt
      181 aacattactt tatttacata tagagcataa taattatcac tataaaaata ttgatactca
      241 tatcgagggt catctttata atacactttg tatagtattc cttcgtaact tgccttcttg
      301 gctttttctt ttgtaatact ttttatatta tcttttgaaa tatcttgctt taccatatac
      361 ttatcaatat atgtttcagc atctttccat ggtttaacat agtttttata tacaacaaac
      421 cctgttacac ttataatgcc tataattaat actataaaaa taactttata tttcttcata
      481 ttaatcctcc ttcatttatt atttaaaact tgatataatg cattaaaaaa aggatgttat
      541 cttttttatt aaaatatagc atccctcata tttagcatca atatatatgc cccaactggt
      601 aggttaaaag accccactgt cagtactttg attaaattac atgtatgtat atattttttt
      661 aacaaatcta ttatatgtca acttgtctac tattacatat aatctgtaat attacatctg
      721 ctgctttttt tgagcctcct gcattataaa agctttttga tagtttagtt gcattttctt
      781 tatattcatt gtcatttata acttgaaata ctgctttttt aatactttct gacttatcct
      841 cttttaacat aattcctgct cccaaatgaa caactctatt agctaccatt ctctgctctg
      901 aatgttgagg aaataatacc atgggtactc cataatataa actttcatta acactattca
      961 tcccagaatg agtaacaaac acatcagttt tttgaagtat agtaatctgt tcaacactat
     1021 ttttaacctt aaaattattt ggtatatttc ccaaactttc aatatcagta cttctaccaa
     1081 ccgacataat cacatcaaca tcagaatctt taaatgcatt tatacagttt ttataaaagc
     1141 taacattttt attattaatt gtaccaagcg aaatatatat tagctttctt tttctctctt
     1201 taggttcaat aatcaacttt gatactgatg gacctacaaa tgaatactta ctagagaagg
     1261 tttctaccat tggttgaaat tcttttgatg tatatacaat agtatccgta tcattatcat
     1321 tagaaactat agaaataaaa ttttttacct catacccttt tctttgcaaa agctttattt
     1381 ttttattaat acgtcttatg ccaaaaatca tacgtataat ttctataaag ctttgtttca
     1441 tcaactttgc tgtatgttca ttaaattcaa aagttgttgt agagcataca tatggaatat
     1501 taagtttttg tgcaaataac tttccccaaa aacttaatga atctgataca atgcaatctg
     1561 gattaaaatc tttaagttct ctacaaactt tttcatctaa agaaatagta gtatctacta
     1621 tcatctcaat taatctagga aaatccttac caattttttt ctcatctcca ggaagtaatt
     1681 ctggaaggta tttatcacaa caaataaatt ttgctccagc accttcaatt ttatctctaa
     1741 attcattaaa agaataatac aatacttcat ttcctctatc cactaattca cgaactactt
     1801 caatagtagg attagtatga ccatatgcag gaatgctaaa aaatactatt ttactcatat
     1861 ttcatacctc cttttataat tttttaaaat cattcttctt taggtactac tcccacatga
     1921 cctataaaat ttatcaaatc acgttgatga acaattccta atcctctttc ctcatctcca
     1981 agtaccaata atttatctcc tgcatttata ttaaatactt ctcttgcttc ttttggaatg
     2041 acaatctgac cacgttcacc aactactact gctccaaaga aaaactttcc tttaggaggt
     2101 actataatac ctgttttttc ttccgaatga ttaactaagt catctagttt gaccttgtac
     2161 aattttgcaa gtttaatgca actacctata tcaggaatag attctccact ttcccatttt
     2221 gctattgatt gtctagaaac gttaagtttt tcagctaatt cttcttgtgt atactggtgg
     2281 atttttctta gacttcttaa attctcatta atcatatttt tcatacctcc tatgtttata
     2341 atattggatt tattatttaa attctatcaa ctaatcttaa cattcatata tatatttata
     2401 tactttttat tagataaatt tattgtttag gtaacatact tccttaagtt ataataaata
     2461 aaagcaagca caaatctatc aacctttgct aaagtattta agttagggtc atttacattg
     2521 ttaaatactt ctacatctta aaaagtttta ctcatcaatc aaatgaaaac ttgcaataag
     2581 aattaaaatt ataataacaa aggtgctgtc tcaaaataaa gataatcttt tttcattatt
     2641 gaaatgataa aaatatattt gattccacgc aaaaaagtgt ctcatgaact aaaaagttca
     2701 ttttgagaca ctcctttttt ataataaaac aatactatct acttcttttt aactatatat
     2761 ctagcgcagc atgatgccct tggtatactg catttgttat tgtagatgca cgaacacaat
     2821 ctccaatttg agcaacatat ggagcacaat caagtaaagt atctaccact tctcttcttg
     2881 gtttttgtcc aagtgcacat attactgaag ttcctggaat atttatttta ttcccagatt
     2941 tattcataca tactacacct tctgttgtta cacttaaacc cttatgttct gtatatacat
     3001 atataccttg tctctctatt tcttctaata atattggacg atgccttata tttgcatccg
     3061 gggcaagttc tgctctcatc tctactaagt gaacagtctt cccttcttga gccaagtgaa
     3121 ttgcagcctc acatcctgct aatcctccac ctaaaactac tacttcgtca gttacttttt
     3181 ctttttctag gtaataatca ttaactatta ctacattttc tccattaaga ccctctattg
     3241 gtggaacaag aggttcagaa cctactgcta taattaatgc atcaacattt tcattatcaa
     3301 catattcttt tgtaactgtt gtatttaaac gtacttcaac acctaaatct tttgctattt
     3361 ttccaaaagt atttcctaat tcatacattt catacttaaa tggaagtgct tgttctccct
     3421 ttaaaattcc acctaattca ttagtctttt cacaaagtat tactttatgc ccacgcttta
     3481 cagctgtaat agctgcttgc aatcctcctg gtccaccacc tgctattaat acttttttag
     3541 attttaatac tggaacaact tcagttccat ctaattcacg tccaataagt ggattaacag
     3601 tgcaacgtct tgtagaagtg gtagcacgtt ctgccataca ggtaaagcat cttaggcatt
     3661 tcactatttt ttcatcttga ttactcataa cttttcttgg taattcgtgg tcagctagta
     3721 atgcacgagc catgtaaata acatctgctt ttccactagc aattatctct tccatcattt
     3781 ctgggtcatt taatgctcct atagtagcaa ctggaatatt tacatgtttt ttaatttctt
     3841 ctgcaagata tacattacat ccatgctcta agaacataga aggatgtgtt actgcaaacc
     3901 ctctttggta tgtacctgct gatacatgaa gtaaatctac tcttgattcc aataatttag
     3961 caatttctat tccataatct aaatcatacc ctccctcaaa taattcagaa ccactcattc
     4021 taaactctat tggaaatcct ggacctactg ctttacgaac actatctaaa acttctttag
     4081 caaaacgaac tctgttttct aaagaaccac catatttgtc agttctttta ttaaagtaag
     4141 gtgataagaa ttgattgatt aaccagccat gtccaccatg aatcataatc atctcaaacc
     4201 ctgctctctt agccaatgct gcaacatttc catatgactc tactatttca gcaattattt
     4261 cttctgtaag ttcttttatc tctaatccat caggacgaac actaggactt accccccatt
     4321 gtgataatcc tcgcttctta tctttgtcaa ctaaataagt tcctgaatat tgtcctgaat
     4381 gagatagttc tacacttggt attgctccat gacgacgaat tgcatctgct gtataagtaa
     4441 aacttgctaa ggaatcaact atttttaagt ctagatgata tgcatgtgaa ccatctgttt
     4501 ctggatgaac catacattca ctaattgtaa ctgcacccgc tccacctttt gctcttaatt
     4561 cgtaaaaggc tgttgatttt gagccaattg tacaatctgc tgttatgtct gttcccccca
     4621 taggtgctga aaacattctg tttttgaatg ttacatttcc aattttaatt ggcttacata
     4681 gattttgata ttttctttcc atcttttata tctcccttca ttaaattttc tatatttaaa
     4741 ttttgtattt tatttatata tctatataaa aatgttaata taaaacctat taaagctata
     4801 attatagcta taatgaaagc cctttgataa ttgccatcta cagaatatac atttttcatc
     4861 actgcaggtc caatatatcc tgcaaaagca aatccaataa acatgattcc ataatttaca
     4921 ctattatttt taggtccaaa tttatctact gtaaatcctg ggaaaattcc cataaatgaa
     4981 ccaaaacata tacctataat tattattcca atatagaatg ttgctacatc tccttctctt
     5041 gagaataaca aacataataa accaaaaatt gaaataacta atgcacctga taaagtattt
     5101 attctaccaa ttttatctga tatatatcct gcaacaatac gaccgaatgt attaaacaag
     5161 gctaaaattg aaactactat tgctgcatct gaacttgtca tgccaatcat atttgttgct
     5221 aaagaagatg ctaatgacac acacattaat ccatagaaag ctccacaagt taatattata
     5281 atcattaaat aaaatatagg tgttaaaagc atttctttcc aatttttatt aataataact
     5341 cttacttttc cctttttctc tggtgctacc catccttctg gtgtgaaatc tgctggacat
     5401 tttttgataa agaaagaaga aacacataca attataagaa atattaaacc tattgattta
     5461 aatgcagagg ttactccata tttattaatt atccaattag taactggagg cattattact
     5521 gaactaaacc cataagctgc tgttgttata ccaccaacta atccactttt atctggaaaa
     5581 aatttaattg aattacttat tgtacatcca taaaccatgc caaccccaag acctgttatt
     5641 atcccatatg ataaaatcaa catgggcaat ccttttgaaa aacctgccaa tatcatacct
     5701 ataccaaaaa ttaatcctcc tacaaataca acctttttag gaccaaattt atcatttatc
     5761 attccccctg taatcatagt aataggtcca actgagtttg taactgtaaa tacaattgct
     5821 aaactagcag ctgtaaaagt tgttccagca aaactattta aatattctgc cataggtgat
     5881 gcaaatacac tccacgcata tattgaacca atacataaat tgatgaaaca acttgcaatt
     5941 aatataatcc atcttttttg tgttaaattc attactctcc tcctcgataa atttattaaa
     6001 tagaatttct tagttaagat tatcacaatt ttttctatat gtaaatataa acatgtaaat
     6061 gtttacatgt ttatatttac ataatttcat ttttatatat agttttattt attttttgtt
     6121 actcataatt aaaaagttct tattaaaaca tagctatatt aattaattca aactaaatat
     6181 atcgtgttat aataagtatg taattaaaga aagaggtgta tttatgcgaa ctttaaagta
     6241 tgcaatttta ggattattaa atagaaatcc tatgactggt tatgatatcg taaaagagtt
     6301 caattttcaa ttagctgagt tttggaatgc aaagcatagt caaatatatc ctgaactaaa
     6361 aaagttggta aatgaaaagc ttattgtata tgatattaaa attagtggtg atgtattaga
     6421 gaaaaagtta tacacaatca caaaaaaagg taaagatgag ttcttgaaat ggcttgaaaa
     6481 ggatgagcca atggaaagaa cccctaagga tatttttcgt cttagaatgt atttttctaa
     6541 taatttagat atagacacta ggcttatttt actagagcat caattgcttc aacaccaaga
     6601 aagaattacg ttcttgtaca gtcaaatgga gcgttatagt gaagttcctc ctcttgatag
     6661 taatgatttt ggtgattata tagttcttga tggagctatt atcagggaac aaggtgctat
     6721 aaaatggcta acaaattgta ttaaccatca taaaaatttg gataatacta ctttgaaata
     6781 ataacttatt gattttaata gtgtatttaa ttatatacat taaatttata aaaaagactg
     6841 tacacaactt tgtctttgta tacagtcttc tatagttgat tattcaaaat agataattat
     6901 aatatttaaa acagttaata taaaagttaa aactttttat taagctatta attttatctt
     6961 cttattgtcc ttcaaaatta cctttatatt tataatcttt cacataaaaa ttactatctc
     7021 ttctcatata aaaatccagt tgatgtcttg ttactaaatt taatggtaat caaattcttt
     7081 accaataagt ataaaaatta ctaatgtgtt ataattttct taactactaa attttacata
     7141 catttgaata gaatatatca ataaattact ataataaata aaaaattaag agttaaaata
     7201 aaaaatagtt agggagatat tattatgatt ttatatttta gcggtactgg taatagcaga
     7261 tatgtagctc gtaaaattgc acaagaatta aacgacgaac tgatatcact aaatcaactt
     7321 ataaaagaag aaaaaacaga tgaattaata tcagtagata aaccattcat attcgtatgt
     7381 cctacttatg catggagatt gccaatagtg gttacggatt ttataaaaaa aacaaaattt
     7441 ctaggaagca acagagttta ctttgtaatg acttgtggag gagatacagc aaaatccata
     7501 aactatattc aaaaactatg taaatataag gaatggcaat taaaaggtat ggcagaaatt
     7561 aaaatgccag agaattatat agctttattt tctacaacag ataaagaaac atcaaaacaa
     7621 atgattaaag aagcagataa acaaatatat aggattattt ctgatatacg caatgaaaat
     7681 gaatttgaaa ctattactcc tagtggctta ggaggtacaa ttaaaagtgg tataataaat
     7741 acagtttttt ataagacaat tattagtgcc aaaggatttc attatactga caaatgcatt
     7801 ggctgtggaa aatgtgtaga gctatgtcct ttaaataata ttaatttaaa aaacaaaaag
     7861 ccagtttgga aaaataactg cactcattgt atggcatgta tttgtggttg ccccactgaa
     7921 gctattgagt ataaaaataa aactcaaaat agagagagat actatttaga ataatatatt
     7981 cttaatattt aaaccattat aaaatgaact atacccattg ttttatacag ttttacatat
     8041 tttaactgtc tcatgggtat agttcaaaag atagtgtctc aaagtgaact ttttagttcc
     8101 taaatcactc tttttttatt aaatcaagta tactttaatc accttttctt atatcatttt
     8161 cactaatatg ttgtattcca acaaatataa gatttattag ctctgttagt gacttccatt
     8221 gtgatttttt tgaatcaaga taataataat ttttcgttcc ttctctacgc aaattaacaa
     8281 tctctgcttc ttttaatatc ttaagatgat gagatacagc aggacgagaa agatgtgttt
     8341 tttctgtgat ttctcccact cgtataccat taaaatcact ctctaagaga gcaattaaaa
     8401 tcaattgtct agtctcatca cccatagcag agatagcttt gcgacattct ttaaatttag
     8461 acgttatttc ttgcattctt tgttgacatt ctttttccat aaaatccacc ctctcttata
     8521 agtatctaac agttgctact tctttctgtg cagtacgtct atatttaaca tctgggtatc
     8581 caattactag tgttgtaaga acatgattac tgcgttttag acctaaaagc ttttttaact
     8641 ttgacgaatt atttgctaca tctgaaaaat atccactaaa taaaacacct agaccataag
     8701 actctgccat taatgccata tttgatgctg caagtgaacc actaatctta tcttttgtaa
     8761 ttatcattat tgcaatagga gcatgcttaa agaaaaagtt atcatcaatc tcaacttcct
     8821 ttgaatattt aatagctata tttgcaatag gttttatttt tctaaaaaat ttcactgctt
     8881 cgttttcata aattgacttt ttattgtcta aaacaatata tgatacatct tgtgaatttt
     8941 tagcactagg ggtataccta cctgcctcaa taatttgctt tacaatttct gaagaaactt
     9001 ctttgtcttt aaatttacgt atacttcttc tagatttaat tgccattagc aattcgtctg
     9061 aatctaactt tggcttattt gtaagttcta taggaggttc atcaaatcct gtcagggtaa
     9121 tagcttttgt gggacagatt gctgcacaat gtccacacat tagacaatct tgtcttttaa
     9181 ttactgactt tttattttct ataattatat tgtttactgg acaatcattt ttacaaagcc
     9241 cacatccaat acaaagttct ttgtcaactt ctattatatg ttggtatttc atattcattc
     9301 atctaccttc cgtttattgg tttaaactat tataacatgt agacattttt tatattcatt
     9361 ggtaaagaat ttgattacca ttaaattttt aatttctcta ttacataaat ttttcataga
     9421 attttaaaat atcatagttt atgcataata aaaaagacaa gcttaaaacc taagcttgcc
     9481 tttttcatct tttattacaa aataaattgc acttacttcc catacttcct aacaatttta
     9541 ttttttaaaa ttttatctgt gaatcatact atccataaag tacaatctaa aataatcctg
     9601 tgttgatttt ctattttaaa aaatgttaaa aattatactt attattaatt tttatactct
     9661 tcttatttaa catagatttc ttgtctaata taaatcttca ccatttgaag caattacttt
     9721 cttataccat tcaaaacttt tctttttcat tcttcttaga cttccattac cactattgtc
     9781 tctatctaca tatataaatc cataacgctt ttccatttcg cctgtaccag cacttactaa
     9841 atcaataggt gcccatgtag tataacctaa taaatcgacc ccatctatag aaactgcatc
     9901 atgcatagct tttatatgtt ctcttagata atcaattctg taatcatctt ctacataacc
     9961 attttcatca gctatatctt tagcacctaa tccattttct acaataaata gtggcttttc
    10021 atatctatca tatacatgat ttagtgtaat tctcaaacct aatgggtcaa ttggccatcc
    10081 ccattggctg tatgaaagat atggattttt agcagatgta aatatgtttc cttcagccat
    10141 atcatctgca ttttcatcag ttctaacaca acggctattg taatatgaaa atgaaataaa
    10201 atctactgta tatttattca ataattcaat atcaccaggt tccatttctg gtgtaatacc
    10261 tttttgctcc atatacttta acgcatagtt aggatattta cctttagatt gtacatccac
    10321 aaagaagtaa ttttctctat ctaactttgt agcttcccat acatcattag gattacaact
    10381 ataaggatat acacttccag ctgcaaacat acatccaatt aagttatttg aatcaacttc
    10441 atgtgctatt ttagttgcaa ttgcactagc tacaagttca tgatgagcag cttgatattt
    10501 tatcttttca acatcttcac cttcttcaaa gcaaatacca gcacccataa atggagcatg
    10561 taaaatcata tttatttcat tgaaagttag ccagtacttc actaatcctt tatatcttct
    10621 aaaaataact ttgcataatt tctcatagaa acctatcatt ttacgattac gccatgcacc
    10681 atacttttta actaaatgca ttggacaatc aaagtgtgta atagttacta aaggctctat
    10741 tttatatttt ttacactcct taaatatatt ttcataaaat tgaagtccct tttcatttgg
    10801 tgtttcttca tcacctgttg gaaatattcg actccatgca atgctcattc tatatacttt
    10861 aaatcccatt tcagcaaata actttatatc ttctttataa ttatgataca tatcaatacc
    10921 agttttagct ggataataat gctcatcatc aaaatcaaac atttttagtt tccctgtaat
    10981 aactgctgtt ctatcttttc ctgttggaca tacatctaca tttgccaatc ctctttcgtc
    11041 ttcattgtat ccaccttcac attgattggc tgcagtagct cctccccata aaaaatcatt
    11101 cttaaaactc atagtcaaag cctcctaaaa ttataaccaa atcactagct tatgctaata
    11161 cctcaatctt tactaaaata agactgtaat tagattatct tctaattcaa tatttttact
    11221 ttctgtttct actacatcta aaaattgtga tgaatttgta ataacaattg gagttacagt
    11281 actatacccc aactctttta ttgcaccaac atcaaagttt aatatagttt gcccttttgt
    11341 aattctatca ccttgtttta tacatgcctc aaaacctctt ccctctaatt gaatagtatt
    11401 taatccaata tgaataagca cttcaacacc tgtatcagaa agaatcccta tagcatgtag
    11461 actaggaaat aaggttgtaa ctaccccatc tactggagca actacttttc catcactagg
    11521 tattattgcc accccttgac ctagtacacc agatgcaaat gctgcatctt ctatatcact
    11581 taattttaat acttcacctt ttataggact tgttactatt tctctttcta atttagtaga
    11641 cttgacttct tttatttctt ctttcccctt atttcttatt tttttagact cttctacttt
    11701 ctcatcttta tataaaatca tagttaatac aaaagctacg accattgata tagctatacc
    11761 agaaagagct actattaagt tacccattcc tccatctggt tcaatcattg ctggaagttc
    11821 aaatattccc atacctccag ttataaattt tctaagatta aataatccgt agaaagcgcc
    11881 tccaatacca ctagcaatac aacttattat aaatggtttc tttaaaggca ataatattcc
    11941 ataaattgct ggctctgtaa ctccaaatat accagatata aagtttggta ttgccatctc
    12001 ttttagtttt ttatctttag tcttgaaaaa tattgccaac acaactgccg aacttgcaaa
    12061 tgtacaagca aaaaatggca tcataacatt atcatatcct aaagtcataa cattatttat
    12121 atatacagga ataaatcccc agtgcatgcc aaaaataaca agaatttgcc aagtaaaccc
    12181 tactatagca cctgccaata gtggactaaa atctcttatt gaaattacaa tttgtgaaat
    12241 aagtgtagaa ccaaatgttg ctataggacc tataactata agtgcaacag gcactgttat
    12301 taataatgta agcattggca caaagaaaaa cttaactaaa tctggaacaa acctattaaa
    12361 taatttttca cacctagaag caaaatacac tataaatata attggtacga ctgttccagt
    12421 ataatcaatt gatacaattg gtagtccaaa gaaatcaata tatactggtg atgcaaacat
    12481 tgttccttta aataaagtgt acatcacatc tgttcctgct gatatactgc tcccttgtat
    12541 agcaggataa catactgcag ctcctagtgc taatcccaac attggtttta atccaaactt
    12601 cttagctgaa gtatatccta agaacaaagg aaggaatgta aataatgcat ctcctgctgc
    12661 atttattacc atataaccac cagatacatc actatataat cccattgcta caaataatgc
    12721 attaaatcct ttcaacatac cacaagctgc cattattcct aacactggct gaaaaattcc
    12781 tgatattata tctatacctt tatctagtat attgcctgat tttttagttt ctgaacttgt
    12841 acttgaatca tctaaaccaa ttatttcaac tacatcttta tagacagctt caacatgatt
    12901 tcctataact acttgatatt gtcctccact tttcattaca gtaacaatac cttccatgtt
    12961 ttttaataca ttgtcatttg ctatactttc atcttttaaa gtaaaacgta atcttgtaat
    13021 acagtgtact aatccactaa tattttcttt tccaccaaca ttttttacta tctcttgtgc
    13081 taattgttta tattttccca tttttatcct ccatatttcg tttaaattaa ttttttgaaa
    13141 tttgaaggtt tggccaactc aatgttacca tccaaccata ggtataattt tgttagtatc
    13201 actcctttca atcattctta atatactact gaaaaattat ttcacttaca tatttgagtc
    13261 tttaactact ttagctaaat gtattgttaa acatataagc tcatcatctg ttaatatgta
    13321 gttatattgt tttttaatga aatttttaat tttaactaca cattcaaact ctttattata
    13381 tttagattta ataatgctta gtaattcttt gtctgtctca cctttatgtg cactatctaa
    13441 aagcaatctt tgtgcaaaaa actttaaatg cattataaat ctataataaa acactgattc
    13501 ttcatgaaat tctatcctaa aatgatacct aactatgcta agtacttctt gtattaactt
    13561 cgtaatatca gatgccaaag tatgaccttc acttaactga gcatttacta tatgaaaggc
    13621 tataaatcca gcctcatctt ctggtaattt tatacctgtt ttgttttcaa taatatctag
    13681 tgctttcatt ccaatttcaa actcttcttt ataaaatctt ttaatttccc aaagaattgc
    13741 attttttaca tttattcctt ctttcattct ttgaattgca gaatatgtat ggtcacttaa
    13801 ggatatgtaa atactttcat ttagcttctt gtttaacttt atttgtgcat attctataat
    13861 ttcacttgat aatttcatgt gttctatagg aatattattg agtaattctt gtagcttgtt
    13921 tgatatttct ttatttgata ttctaaatac tttatttact ttagttttat ctattagatt
    13981 gccttttgat ttttgataac caattccttt acccataacg ataacttctt catcattatc
    14041 atctaatgtg attacaacat tattgtttaa tatcttatta attatcatga ttatcctcct
    14101 tttaagcata aaaaaagctt atccataata atgggcatac tccttcctag gatactgcta
    14161 ttattaatga ataagcttag cttgtactta aacaattact acctaattcc aattaatatg
    14221 ataaaacatc agacatattt tgtcaacttt ttattccata tatttcatca atcttttgtg
    14281 ttactttttt gttaattata ataattacta tcattaacat taacttattt ctttacatca
    14341 atatagatat aaaacaaatt atactcaata ctaataaaaa tactattaaa gtaaatagtt
    14401 gatattatac tctatccact acattatctg tactttcctt aacatttttt tcaactccaa
    14461 aaacatatgt cataataaac ggagcaacca atgctataaa tataactata agtccattta
    14521 ctatatttga aatccccttt tcccctgata aaccaagtac tgccattata ctattttgtg
    14581 gaacatataa agtcacatga gtaattcctg caaataaccc tgcgattcct gctcctatag
    14641 atgctaacat aacaggtttt ttaagtttca atgtaactcc ataaagagct ggttcagtta
    14701 tacccataat agctgatatc catgcagctg atgctaactg tttcatctta ctatctttgc
    14761 ttcttaatgc tacagctaat gtcgcgccac cttgtgctaa attagcacaa acttgagata
    14821 ttaatattaa cgattcttgt cctgtagttg caaatgctga tatgaatata ggagtcaatg
    14881 catgatgcat ccctgtcata acgataaatg gcattgcagc tgctaataaa caaaccatta
    14941 taaatcctaa ccttccttgt agtaaattta ttatactagc taatccctct cctaagaaat
    15001 ttcctattgg tccaattact attaatgcta ttggcgcact tattaataat actaaagttg
    15061 gatttaaaat tgtttttaac atgctaggtg ttaatttatc aatgaatttt tctatatatg
    15121 acatcatcca tactgttaaa agtgcaggga taacagatgc tgcatatata acaccttgaa
    15181 ttggtactcc tataaatgat atagggtccc cacttgatat ccatgcagag aaatttggat
    15241 gtaaaaacac acctactact ataactgcta ttgatatatt aacattaaat tttttagctg
    15301 catttattgc aagtattata ggcataaagt aaaatgctac atctcctatt atatctaata
    15361 ctctatatgt acttgaattt gatgatagcc aattaaaagt ggtgcaaaga gataatataa
    15421 cttttatcat cccagatgct gtaagagctg gtatcatagg tgtcatacaa ccagttattg
    15481 tgtctattat attatttaaa atactatttt ttttattttc attttttata tcgcttttaa
    15541 tatctcctgt ggtatttatc ccctcattta ttattgcatt aaatacaaca cctacctcat
    15601 tacctattat aacctgatat tgagttgaag ttgagtttac tccaactacc ttatctatag
    15661 attttaattt atctgcattg actttctttt catcttttaa gatgaacctt aatcgtgtca
    15721 tacaatgagt taagtcaatt atattattct ttcctccaag tccatcaatt atcaattgag
    15781 ctacatcttc atatttattc ttcatatctt atcctctctt ttaaaataat ttttaatata
    15841 gttttaagcc aaattaatct ttttactcca agcttgtaat agtgtattgt aatttaataa
    15901 gctagtatct tctacaatat ggtcacattt tcctagtact tcttttttcc ccacaccaat
    15961 tgacgtcata ttggcagcat ttatagcttt aatacccgaa caagaatctt ctatccctac
    16021 acaatcatat tgttttaaat ttagcatctt gctcccttca ataaaaatat caggatatgg
    16081 ttttcccctt tctaatagag atggatttac tatacaatca aattcatctt ttattcccaa
    16141 agattctaat attctaggtg cattcttact agcagatgct attcccaatt tcacattatt
    16201 ttttttaagt tcttttataa gcccatttat ccctggtaat atatctttat atgttaatga
    16261 gtttattaac tttatataaa tgttattttt ttcttcacac acctttaact tttcttcttt
    16321 agtataacta tctactttat tagcagataa caatatttta tctaatgatt cttccctgct
    16381 tatgcctttt aatttctcat taaaagtttc atctatttct atatctagcg attttgctat
    16441 ctcataccaa gcttcaaaat gatacttagc agtatctgtt ataactccat caaggtcaaa
    16501 taagactgct ttcatattaa ttactccttt taaaacaatt attaacttct attacattct
    16561 ttgataattt tatttcttta ttatcgtgta atatgcttat gtttttacct ttgattaact
    16621 tataataaga tttatattct tctatattta cagaaactaa attatctcta aacaagatat
    16681 taaatttaat gtttttccag tgtttaggta tcctaggttc aaaacttaat ttatcatttt
    16741 ttattttcat tcctgcaaat ccatatacta agctcatcca tgttccaccc atattagcag
    16801 catgtattcc atcacttgta tttttttgta ggttattaat atccattaat gctgtgtcca
    16861 taaaatagtt ataagctttt tcatgatttc caatttcact tgccatcatt ccaaatattg
    16921 accttgacaa agatgaatca tgagtagtta ttttttcata gtattcaaaa tctctcttct
    16981 tttgttctaa gctaaactca tcagggaaaa gcaacgctga aagtacagta tctgcttgtt
    17041 tatttacttg atatctatat attgttaatg gatgataatt gagaagtaat ggatattttt
    17101 cttttggtgt atttttaaaa ttccatactg atttttctaa aaatgaatcg tcttgtttgc
    17161 ttattttctt ctcttcatca tatggtaaat acatattttg agcggcattt atccagtttt
    17221 ttacttcata ttcttgtaat ttaacttttc taaacacttc atttgaaaat aaattatgct
    17281 taaatttatc taccatctgt actgcatata ttaaattata tttagccatt aaattagtgt
    17341 aataattatt gtttactatt gctgtatatt catctggtcc tgttactcca tttatacaga
    17401 atttattatt ttttgattct atataatctc caaattcaac ccaaaatctt gctgtttcta
    17461 taagaatttc taagccattt tgactcataa attcttcatc attagttgct tcaaaatacg
    17521 ttctaatacc ataagctata tctgcattta tatgaaactg tgctgttcct gctggataat
    17581 atgcactgca ttcattacca tttatagttc tccatgaata taaacaccct ttatttattc
    17641 caagttccct agctctttct cttgatgcat ctaagattga atatctataa tctaataaag
    17701 ctctagcaat ggatggttga gtatatataa aaaatggtaa tatatacatc tctgtatccc
    17761 agaaatagtg cccctcatag ccatcacctg taagaccttt ggctgatata ttagtctttc
    17821 catcccttcc tgctgattga taaatatgaa ataaattagt ttttatcccc agttgaagag
    17881 ttttatcgcc ttcaatctct acatcagagt ttgtccaaaa cttattcatt ttctctctct
    17941 gaatattttt gactttatca aatccaatat cgcatgcttc tcttaaaacc aattctaatg
    18001 attttaaatg cgcttctgat tctaaacatt ttaacgactc cagacattct aatgatttta
    18061 agcgttcttc atttagattt aaatcagaat atactctact atagcctaca acaatttcta
    18121 aatttaacct ttcatctttt ttggcataga cttctatttc tcgttcaata cattcatctg
    18181 aaacattata ggtctcattt gtaatatttc cacaagtaat atacgaatca aacccacacc
    18241 ataaccctaa tttacttctt tctgtaacta aattcataaa atacttattg ttatattttt
    18301 ttatatcaat tttaaattgc ttcttattta tagatgccac tcttgggtca tctacttttt
    18361 cacaattaaa cttctccata ctaatactat tttccatttt atttttaaat tttatttttc
    18421 cattaaaatt caatggcgtt attgtatatg aaatcatcat taattcttct atatcttgtg
    18481 atactaatct ttctatatat actttaactt ttttaccacg agagttttcc catataaatg
    18541 aacgtttaag aataccttca tttaaatcta gtatcctagt atgttcactt gtaatacctt
    18601 ttgataaatc aaagcaatct ccttcaatag agaaatttac tatatgagca ttaggtaact
    18661 tacagatagt ctgattttgt tttgcatagc cataagcgct ttctccatat gtaattggag
    18721 attcttcata aaatccattt acatatgtac ccatattatc tatatactca ttattaaaat
    18781 cagactcttc aatattccct ctaactccta aatgtccatt agctagtgta aacaacgttt
    18841 cattggattt tattgtatcc ttactaaacc cttgctctat aagttggtcc ttagtagtaa
    18901 atattaaact cataataaca ctcctttctt aaaatgaatt atgcatgagt ttccatatca
    18961 ttaaataaaa tttctccatc tgtttctatt acttttttat accaaaaagc actttgtttt
    19021 ttagttcttt taaatgagcc tcttccatag tcatcagaat caacatgaat tatgccatat
    19081 cgttttgtca tctttccttc acttacactg gttacatcta tacatcccca cataagatat
    19141 cctagaagct ctattccatc ttcatttact ccaagaatca tatttttaat atgttctttc
    19201 atgtaatcta tacgataatc atcaattatt tttccatcct cagttacaat atcttgagta
    19261 cccagcccat tttctactat aaataaaggt aactgatacc tttcatataa ctgattcata
    19321 gttattctta accctacagg gtcgttttgc catatactat ttgattttgg aagatatgga
    19381 ttttttatgg tttctggtcc atttgccttt gatttttcta aaccttcagc tctagctgaa
    19441 ctacaagcac tagaataata gctaaaagat acaaaatcta cagtattatt cttaagtgtt
    19501 gataaatctt cctcttttat tattaatttt attttatttg attttaaata ataattcata
    19561 aagcttggat acacacccct aacttgaaca tcagaaaaaa acaaattatg cctttcatgc
    19621 tctaaagttg caaatacatc ttctggttta catgtgtatg gatatgtttt tccagcagct
    19681 aacatacacc caaacttaaa ttctttattt atacaccttc ctatttctat agtttttgca
    19741 ttagctaata attgatggtg tgctgcttga tattttttct gaagttgatt ttcatcggct
    19801 ataaaagtta acccagcacc tatatatgga atatgcatta tcatattcat ttcgttaaag
    19861 ggaatccagt atttgacatc atctttatat cttttcatca aaatttcagc atattttaca
    19921 tataaattaa ccacttttct attagcccac ccagaatatt tttcaactag agaataaggt
    19981 atatcaaagt ggcttatcgt tattaatggt tctatattat atttttttaa ttcttttatt
    20041 atattatcat aaagttgtaa tcccttttca tttggttcac tttcttctcc tgttgggaat
    20101 attcttgacc aagatataga aaatctaaaa catttaaaac ctatttctgc taacattgct
    20161 atatcttctt tgtaattttc acaaaattta atccctatat gacttggata gtattgatta
    20221 ttttcattta aaatattatc ctcatctaaa catacatctt ttaatctctt atcatttaat
    20281 gggagtttat caaattgaga taaacctttt cctccactta aataaaatcc ttcactttga
    20341 ttggctgaaa ttgcccctcc ccataaaaaa tctttcctta atttcatgca tctacctctt
    20401 tcacagtata aaatttcata atttctatct catatttagt tataagattt ttttcatact
    20461 atacctttta aataattcaa tttaaaaatc taattatact ttttatttat tataattaat
    20521 attgtctatt ttcaatactt tcatattaaa tatcaatatt agatatctat gtatataatt
    20581 atatatatag caaatacatt tttatatcct cattattgtt atcttattct catatattgc
    20641 tgtatattta tatatataaa aggatttatt ttgaaataga ggttgtattg agaactaaaa
    20701 tttagacttt ataaatgaat ggaattttca ttgttgaaat actgaaaata tatttgattt
    20761 catataaaag aaagtgcctc atgaactaag ttcattttga gacaccccct tttttctcca
    20821 agctattata tcttttttta aattctaatg gagaaactcc aattttttct ttaaatactc
    20881 tgtaaaaagt ttttgtactt ccaaaaccaa cttcttcaac caaatctaca atagatatat
    20941 tgttattaag taatatccac tttgcttttt ctaatctata ttcatttaaa aaagttataa
    21001 atgtttttcc tgtatttttc ttgaaaaatt tagtaaaata atatacacta aatcctactt
    21061 cataagacac atcttctaat tttataggtt gtttataatt tttttctaca tatttgaata
    21121 tgttgttcaa tctctctaat atctctttag agtttatttc atattctact gtattttctt
    21181 caatttctct aggaatctgt ctatataata taggaattaa attatacatt ttacctttta
    21241 ttgcataact atatccttct attttgtata ggtcttcatt gtagatatct atcaatatat
    21301 cgaaaactga cttgcttatt tttgctgacc aatctctact acattttttt atactactga
    21361 ataaattaat taagctttct ttttcttcat ttaataaaat taaatcatta aaaaaagata
    21421 tatcaaattg aaatactaat ctttcacttc caggtgatgg cagtatataa tgaatatctc
    21481 ctccttttat tatcactatc tcgccttttt ttaattgcat aggcttgtca tttataaata
    21541 gatttactac accttctgtt ataagtaata tttctatttc cttatgccag tgtggtgttg
    21601 ttaatacttt tccttcattt gatagactac gaaaaggaaa ttcatgtatt aatttaggca
    21661 tttctaaata tattgccata gatatcctct cctctctagt gtctaaaaat taatctatac
    21721 tattttaaag aatacacacc tattttaaaa gcttcaaatt tataatttat acaataccta
    21781 cacttcataa aatatatcta atatttcttt ttttgtatgt gcatttaaaa gtctttcact
    21841 tatattttta ccacctaaaa tgtaagctat tttagatatt aatatcatat cttcattacc
    21901 tgcagattgt ggtatagcaa acataattat tacctttaca ggtaagccat ctatacttgg
    21961 ccaatctaca cctttttcag atttacctat tactacagat gccttcttta catattttgt
    22021 ttttccatgt ggcatggcca tttgataacc tatactagta gagcctattt tttctctacg
    22081 ttttagtgcc tttataaact tactttttaa agttatactt cctgtatcgt ataatttatc
    22141 tactaattcc tgtattatgt cttcttttgt agttgatttc aaatctaaaa taatgctttc
    22201 ttctgttaat atttctctaa tttccatttg ttttctccag tagattattg tcaagtaata
    22261 ttttaatatc ctctttattc ttgcaatcta ttatatagtt tatatagttc ttttgcctat
    22321 ataaatttga tatttccatt aaaatattct ttaaaaaact ttgattacta atttctaaat
    22381 aaatatcaat cgttctatta acattattga tattcattat ccctttattt aatttacttt
    22441 gcttatgtat atatatattt aaatcttttc ctaacgaaat agattcatat ttactcccta
    22501 aattcacacc tattaaactt tccatctgca ctacatcaaa attaaaatat atattatctt
    22561 ctaaatacat tcctaataag tttgtatcta agttcatact ttttttaggt ttagaaagta
    22621 aagcattttt tatattatta atatcacttt ccattaatag tactgataca actatatgta
    22681 atttatcttt caaatctaag tttacagtag atattataaa gtctacatca tctaaatttc
    22741 gcttactcaa ctgatgcata ggaactatat caactatagt ccattcagga aattctcttt
    22801 ttaatctggc tgcaaggagc tgagatgttc catatcctgt atgacaaact actattactc
    22861 ttttactact catatttctt tctattgccg cttgaaaata tgtggcaaga tacgaaactt
    22921 catctatact aatattcttc agattatcat actctgtcat aatatttata gtcatcatac
    22981 ataatcccag cacatctgaa aatctctctt gtatctctcc taataacggg ttttttattt
    23041 ggatatcata tcttaatctg tttaacatag gtcttacatg agatgctaga cttttttgta
    23101 gtaagtcatc attttttaga tttatattta aaaattgtga catattctct atcattatag
    23161 atgaaacctt ttcactttca cttaatctat ctgattcatc attgtaacta ctattacttt
    23221 cgctactaaa tccagatgat accaaaaaaa tatatatgta cataatttca tctttcgtta
    23281 tttttactct gtatctattt tctattttat ctataagtgt agtgacattt ttgtatacta
    23341 actcatccaa attatctaca gaaacttctc tttcttcctg agattctatt tgattacctt
    23401 cttcgattct ctttaaacat ataagtatat gggtaattaa atttatatag tagggctgat
    23461 ttatagtata tcctaaattg tattctaatt ctgtaattat agtttctaca aatataatag
    23521 aatctatatc aaataaattt atcaatgcac taaatgtaac agaatctagt cttgttaact
    23581 cagatatatc atgttgttca taattttcat ctaataaatc atccatcatt tttgcaatcg
    23641 atttccttat atctacttct ttacctataa tcttagttcc ttctaaagtt ttattaagtt
    23701 ttaaattgta ttcttttatc catgcctcta tatatttaaa atcatttact atagaagttt
    23761 tacttacata atatttatct gctaactgat ttattgaagt tatattattt gaatttagta
    23821 ataaatactt taaaatctcc attcttctct gctctactga taaagattta ttttccttcg
    23881 attgtatatt taaactatta attaagtcta tgttcttctt tgctaaatct tctattaata
    23941 ttccatagcc tctttttata tcaattttta tattaaaagt ttctaaatat ttctcaatca
    24001 tcttcaaatc tttttgtaaa gttttatcgg atacatttaa tttatcttta aaatatttta
    24061 taggtctata cttattttcg cttaatatca atttaagaaa ttcaatttgc ctcgacacaa
    24121 gcatacttag ccctccctat ctatgtttat atatttctat gtttatatta agtactattt
    24181 taacatattt actaactatg taaaattact aagatacttc tgtatcataa aagaatttat
    24241 gatacacctt actaaattga tataatagat aatataattt caaaataaaa agtaaggata
    24301 tttagaagta gttaactgat tctaaatatc cttattgtta ttctatatac tattattcta
    24361 tatacttgtt gttattccat tacttatttt tactattatt tataatttaa gcacccagta
    24421 attcaacttc actattttca ctccttatag taggctttgt tacccctata atcaaggctg
    24481 ttattactgt tccaatcaat atacaagcta tatatgcaac tggtgcacta catgcaaatg
    24541 gtattacaaa tattcctcca tgtggtgcca ttaaagttat atctaaactc atacatattg
    24601 ccccacctat acaagaacca attatatttg ctggaatcat tcttaatggg tctgcagctg
    24661 caaacggtat tgcgccttct gttatgaaag atgcccccat tatagcagtt ggtagagatg
    24721 actttctttc ttctttagta aacttatttt tgaatattaa catagctaat gatatcgcta
    24781 atggaggtac cattcctcct accattaatg ctgctgatgg ttcaaatata ttacttgaaa
    24841 acatagccaa tccaaaagct gaagctgttt tattaatagg tcctcccata tctgatgcca
    24901 tcatccctgc taatattgca cctaataata ctttattagt tccagacata ctttgtaacc
    24961 aagattgtaa agcttcattt aaccatgatg ctggtgtatt aactacaaag aacattatag
    25021 ctccactcgt aaatgcaatc aaaagtggac atatcaatac agcatttact ccttttaata
    25081 catctggtaa attttctgtt atcttcatta ctcctaaagc tatatatcct gttgcaaaac
    25141 ccgctaccat accacctata aaccctgaac ctccaataac tgccataaaa ccagctatca
    25201 tagcaggagc aaatccttgt cttcctgcta ttgatttagc tataaaacca gcaagtatcg
    25261 gtataaataa cttaaatgct gaatcgcctc caagttgtcc aaagaatcca gctatagggt
    25321 tgtatgtttc atgagttggg tctgatgcaa atataccaaa cataaacgct atggctatac
    25381 caatcccacc tattactaca aatggaatca tttcagatac accactcatt aagtgcttat
    25441 aaacaccctt tttctctgac ccttgaagta tttcttttga atcttctaaa gtctttccac
    25501 ctggtttaat ttctttagcc attccatttt cagcgtttat aattaaatct ttagctaata
    25561 aaacattctt agcatctact actttctttt gtaacacttt ctttcctata aatctactca
    25621 gttgaacttc tttatcacaa gctattatta cgtattcagc tctttttata tcttctttag
    25681 ttaacttatt ttctacacct atagctccat gagtttctat tttaacttca tatccaagtt
    25741 cttgtcctgc ttcctctaat gctttttttg ccataaaagt atgagctata cctgttggac
    25801 atcctgttat cccaactata agacctttat tttgattatt actaaaatct cctgattctg
    25861 cagctttatt attaaatata cttaatattt cgtatggagt ttctgcactt agtagatgct
    25921 taagaaaatt ttcttccatt agatatgtag ctagtttaga tataatctta agatgttcat
    25981 ctgaacctga ttctggaact gctatcataa aaaatgacct aagtttttga ccatcattat
    26041 ctgtaatctc tctatctgtt cttaaataca tcattacagg ttgatttact acatttgact
    26101 tagcatgagg tatagctatt aaattaccca tattagttga tgattctttt tcacgtttat
    26161 taatatcagc tataaacttt tctttattat ttatataatt tccatggtac aaatgatttg
    26221 ccatataatc aatcacatct ttttttgttt gtaattgttt atctagtaca atacaatcgc
    26281 ttcgtagtat tttacctatc ataaattctc ccctcccttt aatttgttac ctcataatta
    26341 attcttattt ttataaaagc actttaactt atctatagca ttgaataaat tcattttaaa
    26401 ctctataact tctcttgcaa cttttataca tggtggatat atagtatctg gctcatataa
    26461 gcttgggttt tgttccaata gttctctgca cttcttataa tatgccgact taacatcact
    26521 cgaaatattt attttacaaa ctcctagttt aactgcttgt gctatttctt catctggatt
    26581 tgatgaacca ccatgaagaa ctaaaggtat atctactact tctcttattt cttttaataa
    26641 atctagttta agctctggtt taaaaccttt tggatatata ccatgtgctg taccaattgc
    26701 aatagctagt gtgtctactc ctgttttttc aacaaaatcc ttagctaact ttgaatctgt
    26761 atatattatt tcatcagtac caccttcact tgatgttcct gttgttccta ttgttccaag
    26821 ttctccttct acggatacat ttaatccttt tgctatttct actatttccc tagatatagc
    26881 tacattctct tcatatggta aatgtgagcc atcaatcatt actgatgtaa atccacatct
    26941 tattgccctt aatatttgtt ctttattttc cccatgatct aagtgtatca ccataggtac
    27001 tttactctta tttgcttctt ctatgcaact agctacaaag ctatctccta gaaaacttag
    27061 ctcatttggg tgtattgcaa gtattactgg tgcatttttt tcgttagcac tctgtactac
    27121 tgctttaagt atttgcccac ttcctatatt aaatgctggt acagcaaatt gattttcttg
    27181 tgccactttt agcatttcct tcatatttat taacatattg tacctcccga tattttaaat
    27241 tttaggttga atttgcttct tgttaatttt gttgttgttt atatttaaat aatatcagga
    27301 taatgaaggt cagaaaataa caatcgtttt ccttagtgaa aaggaacttt tattatacac
    27361 tctaagttcc ttttcactaa ataattttta gaagtctgaa tcatagttaa ttatatttct
    27421 catgtacatc ttctcttttt aagtgtttta tgctttagct ttcttaactc tttctaataa
    27481 acaattagat aatttagcta taaatttaaa gatattcgta tcgtagttag tactatattg
    27541 gaatcaaaaa aattaaaaag atactagata gcagatacca attactaata caccataagc
    27601 catagatact tctatcttaa attcttgcat attagttaca atagaaatta aataggacta
    27661 ttataaaaac cgtaaggtaa ggctgacatt tattctccaa ctgcacaaat gaatactaat
    27721 atagctcctt ctaattaaat catactatat aaatcatata tatcccaatt tttaacctta
    27781 cctctttata tttacttttt aataaaaaaa gatattgact aaatagtcaa tatcttttag
    27841 tggatttaaa tgcccacgat tttaatctaa tttctaatgg tttccttagc ataatcttat
    27901 aaatattaaa atttatatac ttataaaatt tctccattaa attttattac tttcttatac
    27961 caataaaaag atttcttctt atatctttta agagtcccac ttccatcatt atttttatct
    28021 acatatataa atccatatct ttttttcatc tctcctgttg taaatgacac tgggtctata
    28081 catccccaaa cagtataccc tataacatca acaccatcaa tttcaatggc atttttcatt
    28141 tctcttatat gattagccaa atactctatt ctataatcat catctacagt attatctgat
    28201 tttaatatat cctctgctcc aaatccattt tcaacaatga atataggaag ttcatactta
    28261 tcatatagtt gattaaggta aaatcttaga cctacagggt ctatattcca tccccaatca
    28321 gaagttttta agtatggatt ttcaactaca tcagcctttc ctgctgagtt attaccggtt
    28381 gttttcatag ttttatcagc tgttactaca gtagttaaat aataactaca tccaataaaa
    28441 tcaactattc cttcttttag gattttttta tcttcctctg taatatctaa attataattt
    28501 tttctttccc ataattttag tgtatttctt ggataatgac ctcttacatg tacatcagta
    28561 aacataagtt gttctctatt agattctaca aatttaatca tatcctctgg attgcaagaa
    28621 taaggataac ttggcatagc tgccaccata catccaattt taaactcagg gtttatttca
    28681 tgacctatct tcacaacctt tgcacttgct acaaattcat aatgaactgc ttgatacata
    28741 acttcttctt tgtcttcctg ctcttcatag attattcccg agttagtaaa tgcataaata
    28801 ggatttgtag ttatactttg attatttatt tcattgaaag tcatccagta cttaacttta
    28861 tccttgtatc tactcattac cacttcacag aagtttacaa agaaatctat taattttcta
    28921 ttcctccatc caccatagtt tttcactaaa tgataaggca tttcaaaatg gcttaaagtt
    28981 attactggct ctatattgta ttttaataat tcatcaaata aatcatcata aaatttcaat
    29041 ccatcttcat ttggaattga ttcatctcca tttggaaaaa tcctagtcca agcaatactt
    29101 gtcctaaaac acttatatcc catttcagca aataatctaa tatcttcttt ataattttca
    29161 taaaaattaa tggcttcatg attaggataa tttaagccct caattattcc atctgttatc
    29221 tttcttgaaa tagtatgagt acctgcagtc atcacatctg caatacttat tccttttcca
    29281 cctttattat atccaccttc aacttgatgg gcagcaactg cccctcccca taaaaattct
    29341 tgattagatt tcatagaaat atctcctcca gtataattaa taaacaactt ttattacagg
    29401 cgttccaatt ttaatatgtt tattttcttt aactattaca tctgccaaat catttgtgtt
    29461 ggttagtatt attggtgtaa caatactata cccttcacct tctattttat ccttatcgaa
    29521 agaaacaatc ttatctccct ttttaacttt atctcctgca ttcatatgag tagtataata
    29581 ctttccatct aacatagtag tgttaagacc tacatgtatt aatatttcta cacctttatc
    29641 agaggtaatt cctattgcat gctttgtagg aaaaatagtt gttattgttc catttacagg
    29701 agacacaact tctccctttg aaggtattat tgccatgcca ttacctatag ttcctgatgc
    29761 aaacacttca tcttctactt cagataactc tatttgtaag ccttctattg gagataatat
    29821 ttcctctata ccatcatctt catcaaaagc aacttcttca ttaccttctt cacattcttc
    29881 tttactcaat ccaaagaaat atgacaatac tgctcctagc actaatgctg ttcctattcc
    29941 taatatatat cctaggaatg gtgtataaac tggcattgag aatatattat ggaatacata
    30001 tgcattacaa gtaacgccaa atacaccaca tataccacca ccaatacctc ctgcaatagc
    30061 caatactgga atcattcttc tgtatcttaa tataattcca taaattatag gttctgttac
    30121 tcctgctaaa attcctgtaa ctgctgttga acctgctaat acttttaatt ctgaattttt
    30181 tttagatttt ctaaccttca taaataaacc tattgcaata cctatttgag caaatactgc
    30241 tgcaacagct gctccttcta ttgggtctcc acccattgta gccaagtttt gtaaagtaat
    30301 tggtgtgaag ccccagtgta atcctaatac tgctaagaat ggcatagcta tacctattgc
    30361 agctcctgca attactctgt taaattcaaa taatcccata aaaatattgg atattgcatc
    30421 accaacatta gttgctatcg gtccaaataa aatcacagtt aaaggaacca tgactaataa
    30481 tgatatcatc ggtactaaga ataattgtaa atcttttaat ataatcttct ttaaaagctt
    30541 atcaacatag taataaataa atatagcaac aaatataggg aatacaccaa aagaataatc
    30601 aattgctgtt actggcatcc caaaaaagtt agcaccacct tttgctccaa ttaaagctgt
    30661 aaaattagga tctaacaaag ctgctcctat cactccacca acatatggat ttgcaccaag
    30721 ttttatagct aaagtaatac ctaagaatat tggtaaaaag taaaatacag catttccagc
    30781 tgctgataat atagcataag tgctacttgt atcagacatc catttcatag tagttagtat
    30841 tgtaagcagt gccttaatca ttcctgctcc agctagtgca ggtattaatg gagaaaatgc
    30901 acctgaaatt gattcaaata ttttagaaac tattgaaact ttttctccct gttcttcagt
    30961 attttgtcct tgtaaaactg ctttttttat aatagtttca aagtaatcag ttactttatt
    31021 tccaattacc acttggtatt gtcctccact atttactaca gatagtacat ccttcatttt
    31081 taataactct tctttgttag tttttgaagt atctttcaac ttaaaacgta gcctagttgc
    31141 acaatgaact aaactaataa tattttcttt tcctccgaca tattttataa tttcttcaga
    31201 ctctctttca aatttcatac aatctatcct ccattttcat aattttatgt ataaaaatag
    31261 acctaaatca atacaattag atattaaaaa ctaattatac caacttaggt catgcctgcc
    31321 aaacagtaac aatcctacac tatttacttt atatttgata atctatttaa atgcaatgtt
    31381 aagtacaaca attcattttc gctaattttc catttatacg tcttttcaag aaagtttttt
    31441 actttttttg cacaattgta atcacttttg tatttcattt ttattatatc taacaatgat
    31501 gtgtctacat ttgtattctc accacttaat tgccttttta taaaacatct taaatgactt
    31561 ataaaccttg tgtagtcaaa ggaatattca tttatttcga ttccataatg atactttatg
    31621 atatctaata cattatcaat tatcttagtt aacaataaag tttcttgcat atcttttgtt
    31681 tctaactgag catttgcaaa gtgtatcgta ataaatgcta tttcagaatc aggaatatct
    31741 ttatctaatt tttttcttaa atactctaca gctttcttac taaagttata ttcattagga
    31801 tatatttgtc ttatttccca ttgaagtggg ctttgaaata ctaatccttc atccattctt
    31861 tttaacatat aacttatatg ttcggataat gtgattaata tactatcatt aagattatca
    31921 ctcaattcct ctcttcccat ttgaataatt tcacttacaa gctcaacatc ttttaaaggt
    31981 atcctatcta gtagtgaatg taattcactt aatgattcct cactattgaa tataaatttc
    32041 ttttcaattt tatctatttc aactaaatct ttagtaaatt tattaaatcc aattcctttt
    32101 cccattaata ttacttcttg tcctgaatta tctctggcaa atacaacatt attattaaat
    32161 actttcttta taaccataaa ctacctccac tgtaaacaaa aagagaccta aaaaagacta
    32221 ataatacttt aagcatttac tagaattttt aggtcacgcc tgattacatc tatctcagta
    32281 acgatccgtt tatttatctg tatgataact ttattttcat tataaattaa taactgtact
    32341 tagtcaatat ataatttcat gtatatctct actagattat tgtcataggt attcactcat
    32401 tctacttttt ttatatgatt tatataatct aaacatgcta aaataagttt tgaagttatt
    32461 tcataacaaa attaagctca tatatttttt attatatata ctcgattatt tatccatata
    32521 taaggaaatc ctttattttt gtaactacaa taactgccta agttttgatt ttcttttatc
    32581 ttttacttct ccttattaaa aaataattct tttactgttg tatctaagtc tctaaacagt
    32641 ttaattatca aatcattgtt tggattatta accttaccta acttactata tagcttttta
    32701 actgtatatc tttaatagac cttttggtat gctaatccat tttataacat taattcttga
    32761 atcaagcata tgttaccctt acaaagcata tcttataccc tctgtaaagt tatgatagtt
    32821 gatgaacctg aagaaattac ccttacaaga catatctgat acaacctcac ttataagttt
    32881 atatgtttca gaagtatata aataacccat acttccttct aaacccttac ctgttttctt
    32941 agaaaattct aatatatttc cttacatagc aattgtaaca gtatcttatt aaatcttata
    33001 aaactataca tcctctttac atttaaactt taaactttta tctatatgtt tattaattaa
    33061 ctgtacattc actatttttt atcatttata atactcttct tattgtctta aaacaattat
    33121 aatctaacct taatcacata ttatataaat atttttaaca aacttaataa ttaaataact
    33181 atatttcatt atccatctta taaatgttta taaactttcc actattcttc ttcaataaat
    33241 tcaaacaaat tttctactgt ggtatcgaat actttaacga tatccatagc taacttcaaa
    33301 gaaggattat atttcccatt ttctaagtgt acaatagtct cacgtcttac cccaactaat
    33361 ttagcaagtt cattttgagt cataaggagt ttttctctgt attctttaat ttttgttttc
    33421 atcaatattg ccacttagtt acatatcccc cttttatcaa agtacatata caacactaat
    33481 ttagataaag actgtataaa taaaactgca atgaggatta tacctgtatc tatgtttgat
    33541 atatttatat tacttgtata aggtgttgca aagaaaatag atattattac tatagaatat
    33601 atcattattt ttattgcaat tttattaacc tttgatagta taaattcaga tgattcatca
    33661 actaaatcat tatatttttt attataaatt gaataacatc caaaaactat tagccaactc
    33721 aaccctggaa gagaagcagt gcttggtcct ttaaaaatat acttagtggc taatacagct
    33781 ataaaaaaat aagttattac aagcaactcc aggataagct ttgattttac accagtttct
    33841 gatttagaca tataaaatca ctccttaaaa attatattct ctatactctt taattttcgt
    33901 ttttatcaat attatcactt agttgcatat tcccttttta tcaaaatata tatacaaaat
    33961 taatttagat aaagattgta taaataaaat agctaagaga attacaccta tatccaaatt
    34021 tgatatatcc aaacctcttg tataatttgc tgaaaagaaa acagctatta gaaaaataga
    34081 atataacatt atttttgatg caatatgatc aacctttaat aacataagtt ttgatgcctc
    34141 gtcaactaaa tcattatatt ttttattata catgaagtca catgcccaaa aagcgaataa
    34201 aattataaaa gtaatccaaa tatttccccc tttaaaaata aacttttcta ataatgagtc
    34261 tataaaaaaa taggacatca taagtaactc tgaaacaaga tttgttttaa caccaagctc
    34321 tgatttagac atataaaatc actccttaaa agttatatat ttataacacc tcatgttata
    34381 aatatataac ttttccaaat aaattgcaat agaatattga aaatattcaa taatatcttt
    34441 ataaatatag tttataaaag attttataat aaacactaca atacagtact aaattaattt
    34501 attgcatcta ttatctcaat catatcattt actaactatt attctaatga gcatataaca
    34561 ttgaaattta taattataca acctaaatgc atcccaatgt accctaaaaa cctcattttc
    34621 tgtaactgaa gtataattgg tagattttat cactgaaata aaaaacacta gtcaataaag
    34681 actagtattt tcaagtattt tatcattctt tttttaaatt attggagacg aatcatcaga
    34741 ataataccca caatttatct atatgcaaat tctaaattta aaataaagtt tcagatgaat
    34801 ttactattct atgctgtgat acaacagaaa aaacttcaat ctataagtgg cagtttcata
    34861 tagtcctaat tcctacaata aacagaaagg aggtttattc tatatataac taatgataaa
    34921 ttttattata ggagctatta gctctatttg tgttgctata agtactaatt acatatatga
    34981 taatttttta atacttatct attagctata gagaaagtag tttagaattt aaatttaaaa
    35041 taaaatttaa gttaactaag aagcaatcta tactagaaac cactactcta ttccactaga
    35101 ttgtagtttt tttgcttttt atatcactat gttatactac attaaattga aaaatattga
    35161 aaaataaatc ttaaatttat ttattacaat tcattaattc aaaaaaatat tttgagtcgt
    35221 gagttttgta aagatttaac catgaattta atgattctga ttcaaaaaca tctaaaattt
    35281 tcaatacttg aaatgtaaat tccaacggag cagttcctgg agctgtaact aaattttcat
    35341 cattgactac agattccatt ttataatatt tttctccaga ataatttggt actaccattt
    35401 taagaaactc taagtcatta cttgtatggt ttctaaaatc taatagccct tttttagcta
    35461 gacctaatgt agctccacat atggctgcaa ctatagaatc ttgtattaaa gcttctttag
    35521 ctttttctaa tattgtctca tgattcatag ataaccaagt gtttccccct ggcaaaatta
    35581 ataaatcctt gctttccata ttaaactcat caactgtaat ttcaggtaaa acttttaacc
    35641 cacccattgt tataatagga tttttatcaa ttcctacagt aattactttt agtggtttta
    35701 atcctttttt aaaatatctt cctgtattta attcagcagt taaatatgcc acttcccaat
    35761 ctgacatagt atcaaataca tacatatata cttttctatt tttcatcttt tttactcctt
    35821 atgtaattaa tttatatatc taattgtaaa ataacttaac tgacatattc agtcagttaa
    35881 gttattaaat ttgataataa tcaattaatt ctagtaaatc ttcaatcatt ttcttcctaa
    35941 ggcttagtgg ttgaattact tgtattgtct ttctataagg taaaagtata tttggtatgt
    36001 acttgcatat tacttcctct tcaagcataa atattatttt attaggtgtt ctttctttta
    36061 agtagtgccc taaaaaccaa tgttggctta aactatctaa agaatctata ctcccttcta
    36121 caactaattg aattatacct tgttcatttt ttatttcagg tataatccct tttataaaaa
    36181 attcacttgc cgaaaattct gtcggtctgt taaatgtatt tgatgtttct atgatttcac
    36241 taatcctatc cactctgaaa ctacgaatct catttctcat attacaaaag gcaactacgt
    36301 accaattatt attccaatga acaattccat atggatttat aattctatat tttgattttg
    36361 ctttacgacc tgtatgatag ttgatttcta cagatagctc ctttaatata ctattctcca
    36421 attttttaag tactgattct atagataatt tcccaattgg ttttattact tcaagagcta
    36481 ttaaatggtt tgttaaaata tcttcttgtt tttgatttga atacattttt aactttaata
    36541 tcgctttatc taatgcttcg tctaagaagt atccttcttt tttagcaaat agagctgcat
    36601 gaagtagagc tttttgctct tctatatcaa aaaaaagagg tgctttagta aaattattta
    36661 aaaggctata cccaccatta tgacctgtat ctgaaattat aggtactcca ctcgcagaaa
    36721 gtacatcaat atatcggtac actgttctta tatttatttc taatttttca gctatctgtt
    36781 ttgctgtaat ttttttgtca gagcttaaca tccacagtat agacatcata ttgtcaattt
    36841 tagccatgta gtttcacctc tttctatttg ttatctaaag ctattatata actttttgat
    36901 tgatttttac aaaaaaatac tatcaagctg ctaataattt ctactaagat ttatttcaaa
    36961 tatttacaaa tagtcttttt ctatagttaa gttatctctt aaattacata gtagtatatc
    37021 tattttagtt gatatatttt cagctacctc ccttgaatat tattacaata agtctaaatc
    37081 ttcttttttc cataaatata ttatgaggaa agactatcat ctttaatggt ttaatagtat
    37141 aaaagatatt aatttaaaaa actctatttt tatatcagat gggtattgtt ttttataaaa
    37201 ctgaaaaaat cacctaatag aatctatgat tttttaacat aacctccata aattagtacc
    37261 aattatcact tcaaattcaa atttttatct aaagaatata caatcatatt tccatactca
    37321 acataatatt gagtgtttgg cttgtattta caaattatta ttctaacaaa aacaactata
    37381 catagattaa aatcatcttc taaacataac tactaaaatt gaattaatga tatcaattcc
    37441 attaatctct tttaataaaa aattctcttt tctatatgaa aattatttca aaactttaaa
    37501 gttaaataca aaaatgacta taaatagatt tttatttaca caaatctatc tatagtcact
    37561 atagtttatc caataaaata ataaaataaa tatgtacaca ataaacgtta tttacgtctt
    37621 aagtatttat ttgttagatt tttaattaaa aaaaacaagc taatgaacat taatgctaaa
    37681 tctagcatta tattgctttg atctcctgtt tgtacatttt gacctacatt actatctaaa
    37741 ctttcatctg tatctatatt tatatctggt tttccatccc catctgtatc tatatttata
    37801 tccggttttc catctccatc tatatctata tttacatccg gctttccatc tccatctata
    37861 tctatattta tatccggttt cccatcccca tctatatcta tatttatatc cggttttcca
    37921 tctccatctg tatctatatt tatatctggt tttccatctc catctgtatc tatatctatg
    37981 tctggttttc catctccatc tgtatctata tctatgtctg gtttgctatt tcctccatta
    38041 tctggatccg gtttgctatt tcctccatta tctggatctg gtttgccatt tccgccatta
    38101 tctggatccg gtttgctatt tcctccatta tctggatccg gtttgctatt tcctccatta
    38161 cctgaatccg gtttgctatt tcctccacta tctggatctg gtttgccatt tccgccatta
    38221 tctggatccg gtttgctatt tcctccgtta cctgaatctg gtttgctatt tcctccatta
    38281 cctgaatctg gtttgctatt tcctccatta cctgaatccg gtttgctatt tcctccgtta
    38341 cctgaatctg gtttatttgg atctggatta cctgtatcat aacctcctga accaatatct
    38401 acatttttat caggcttcca ataatcatct actccatcgt tatctgtatc tattgctgga
    38461 cgattataat ctgtatcagg gtttccatct ccattggtat ctatattttt atcaggtttc
    38521 caaataccat ctccatttcc accttctgta ctaggtttcc attcacctgt attatcagta
    38581 tctatgttaa tatctggctt ccaatcacca ctcgtatcta tattgatatc tggccttcca
    38641 tccccatcag tgtctatatt tatatctggt tttccatcac catctacatc tacatttgtc
    38701 atatctaact ttaacttctt ttcatcctca ctaccttcaa tgtaatttat atcatctcca
    38761 atatatttta cagtaatagt ttgttcagta gtaagaacat gatgattact atctaaaggt
    38821 ctccattcaa tagatgcagt actataatta tgactctcac tacccttatc atgagttttt
    38881 aattctactg gatctttata ttcctttcca ttaatataga attgtacata acctcttgga
    38941 gaggcacaat ctggtccttc tttcttatcc ggtgaaacag tagcatttaa tgtaaacgca
    39001 tctgtcggtt taaaaacatc agttttaact ttagactctt ctacaactaa tgaagtttct
    39061 gcatcagcag gaataatttc tgccttataa tatgctctat gcccccaata agcatcctta
    39121 aaaggatata tactcgaaaa ctgtgttgat gtaatgctta attgatattt tccagcatca
    39181 atttgttttg taaggtcatt acttcctaaa tttgtatctg gttccatagt ttcatcattt
    39241 aaacgttgag aactaatagt cataaatttt ttatctgtga gtttttcacc tttaggagta
    39301 gttactggaa ccccaccatc ttttaaaatc caatctaaaa atcctttttc tacatcttct
    39361 tcaaatggta atccatcata tcttttatta atcaaatatg gtggagtatc atcaatagta
    39421 aattcttcat attgctttaa aaattgagaa cctgaaccta tatctttaat ataaaaatca
    39481 tctggtttta gaatttcccc agtaatttta tctataacag ataaatcaac agatacttca
    39541 gatcccttaa attgatcaga aaaccataag taaagaatcc cttgagcatc tgtataactg
    39601 ggtgctccat aaggataaat ttttccatta attaacattt taaaatcatc aactaatgta
    39661 gtattaactt tatcttgccc taaatacgtt agagatattt tattcataaa aacttttgtt
    39721 ttcttttcta aatcaccgta tgcaactgct ccattgaaac ttttgaatgt tgttgctttt
    39781 aatgaaccac ctgttactat aacctctcct gttgtagcac ttaggtcaaa aatccaactt
    39841 cctgatcctg tcatataagg taaaatcgtt cctccagtta caagtacacg atatcctcta
    39901 tttgttcctg aattaacaca tccatctcca aatgcactac catgtgcccc tccatatgaa
    39961 taggaaagtc caccattaat tgtaatattt ccactataac cctgaccagt attaggatct
    40021 actactgctg gacttaaaac gcctttcaat gagagatagt accctccacc tattccagct
    40081 ccatgatatc cgcctctagc agttacagta ccaccattga ttgtgatatc tccaccagta
    40141 ccgttaccac ctccacctat tccagctcca tatcctgcac tctgactccc tcccagagct
    40201 gtagcattta taataccacc attaatgata atatttcctc cactttcagt atctccacca
    40261 ccaatacctg aagaatgagt atttccgaca actgttaaac tcccacgatt tttactatcc
    40321 agtagtgtaa ggcgatctcc tttttttagc gtgtctccat tttctagagt tacatctcta
    40381 ggaatttttc cattctcagg aactataaac tctccttcaa tatctctatt tacaacagaa
    40441 tcatcaataa ttagagtagt actttttcct gctctcatac ctggataata tatattagat
    40501 gacgttaata tgtttttact atcatcttgt aatataatat gtgcatatgc accaccatct
    40561 ttatctggtg ttaaagtaaa tgggcatgtg gctttaatat ttactccagc aaatattaat
    40621 tcggctttta catctttagg cacaacaatc ctttggtttg ttggagtatt tggattatta
    40681 ttttgaattg tcatttgttt tccactaaga atagttaaaa cgccacttgc aaatttaaaa
    40741 tcaacatcta tttctccacc actaatttta aaatctcctg cagacataga ctcttctgca
    40801 atggcatctt cttctacaat tacttcatta acatcttctt ctgcatctcc tgcattagta
    40861 tctccttctg catctcctgc attaatatct ccttctgcat ttcctgcatt agtatctcct
    40921 tctgcatttc ctgcattagt atctccttct gcatttcctg cattagtatc tccttctgca
    40981 tttcctgcat tagcatctcc ttctgcattt cctgcattag tatctccttc tgcatttcct
    41041 gcattagtat ctccttctgc atctcctgca ttagcatctc cttctgcatt tcctgcatta
    41101 gtatctcctt ctacatttcc tgcattagta ctttcttcta tagtatctgc gaaagtattt
    41161 gaagcattaa atgatgctac tgatccgagt cccattgaga tacttaaaat cattgatagt
    41221 gctttctttt tacatttttg ttgcataaat tccccctccc cctaataatt tttaatatac
    41281 atcgtataca tccttatata tactctaata attcttaata tatgctgtat acattctaat
    41341 acatactcta ataattttta atatatactt aaaacttata tttgtcaatg aatatactat
//
LOCUS       DAEPXF010000014        38018 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain HB27
            SAMN13376389-rid12479423.denovo.015, whole genome shotgun sequence.
ACCESSION   DAEPXF010000014 DAEPXF010000000
VERSION     DAEPXF010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13376389
            Sequence Read Archive: SRR10518432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 38018)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38018)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001069624.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001069624.1
            Genome Coverage       :: 315x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/15/2021 01:54:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,165
            CDSs (total)                      :: 4,076
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 89
            rRNAs                             :: 10, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 10 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 19 of 69
            Pseudo Genes (incomplete)         :: 44 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 10 of 69
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38018
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13376389-rid12479423.denovo.015"
                     /strain="HB27"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="China: Hebei"
                     /collection_date="2017"
     gene            96..2285
                     /locus_tag="KRL67_000673"
     CDS             96..2285
                     /locus_tag="KRL67_000673"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobalamin-dependent
                     ribonucleoside-diphosphate reductase"
                     /protein_id="HBH1521578.1"
                     /translation="MKYIIKRDGTKELFSKSKIEDAVYKASINSEGGVDRDLAYDISR
                     KIANDYNSMEKCLSVEEIQDIVEVLLMESNRKDIAKHYILYRERRSEIRTKAWEFDEL
                     QKSIWKNKYRHNGESFDEWIKRISNNSSRISKLIRQKRFLFAGRILANRGLQKEGKKV
                     TYSNCYVIEPPKDSIEDIFDTAKKLARTFSYGGGVGIDISKLRPKGAKVNNAAKHTTG
                     AVSFMELYSMTTGLIGQRGRRGALMISMDVNHPEIEEFIDIKTDLEKVTKANISVRVT
                     DEFMQAVREKKLYNCTFEVETTGEKIVKIVNAYDLFNKFVKNNWDYAEPGILFWDKIK
                     TYNFMSEDKNFCFSGVNPCAEEPLPSGGSCLLGSINLSEFIIQPFGEHATFDINKLKS
                     CVREAVVALNDVLDEGLNLHPLQEQRDSVHKYRQIGLGVMGIADMLIKMGIRYGSDES
                     LELCDLIGKTMLNEAVKQSALIAKEYGTFSEYKYENISKSKFFIENLNEDTKEMVKKY
                     GLRNSQLLTIPPTGSISTMIGISGGIEPIFNLSYIRKTESLHDEDVYYKVYTPIVKEY
                     MDKKGITDEECLPDFFVTAMNIGYEERINMQKVWQKYIDASISSTINIPFETTVEDVF
                     NIYIKAWEAGLKGVTIFRDGCKRSGILINEKKEENNKENKKENSKKEYYKNNKKEKDN
                     KDKKAKEIINKNFEEDEKFVCPECGSEGIAHTGGCSICLKCGYSGCN"
     gene            2353..3120
                     /locus_tag="KRL67_000674"
     CDS             2353..3120
                     /locus_tag="KRL67_000674"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HBH1521579.1"
                     /translation="MQDKSVREIKEIIETLEVEKYMEYIELLRIDERKSVQGLAIKLA
                     KKLDNIRKEEERLETINIFENDGYDKGYLYIGGIDEAGRGPLAGPVVASVVVFKKDTK
                     IEGVNDSKKLSEAKRDELFEVIKEEALDYGIGIVNNEEIDEFNILNATYMAMKKAINC
                     LKKAPDYLLVDAATIPGIDITQNPIVKGDSKSISIAAASILAKVTRDSIMYQYDRVYP
                     EYGFKSHKGYGTKEHYEAIEKYGITPIHRKSFLKNIL"
     gene            3178..4194
                     /locus_tag="KRL67_000675"
     CDS             3178..4194
                     /locus_tag="KRL67_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HBH1521580.1"
                     /translation="MEYNELLKSARENLNGSCKVCKVCNGVACAGEVPGMGGKGSGSS
                     FIENSKSLEKIKVNMRVIHDVSNPDTSIEMFGKKMSAPIFAAPVTGTTLNMGGKINER
                     DYIEPVVAGCANSGIYAMVGDTAVDAFLMENLDVVKKYNGAGIVFIKPWDNENIIKKI
                     RLAEEAGAFAVGVDIDACGLVTLSLHGKPVLPKNVEQIKELVKSTKLPFILKGIMTVE
                     DALMAVEAGVDAIVVSNHGGRVLDCTPGACEVLPKIADAVKGKVTILADGGVRTGLDV
                     LKMIGLGADAVLIGRPFVTASFGGATDGVETYVNKLQSELSGSMILTGCQTIKDIDGK
                     VIYK"
     gene            4260..4508
                     /locus_tag="KRL67_000676"
     CDS             4260..4508
                     /locus_tag="KRL67_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH1521581.1"
                     /translation="MSRKAYEEALVELEKFIDERKEIIKSAEDCIDKYIVDRTLPFDY
                     KDKCVEWQQELLDIAEAQVLEANELSVLLQEKKELEED"
     gene            complement(4681..5178)
                     /locus_tag="KRL67_000677"
     CDS             complement(4681..5178)
                     /locus_tag="KRL67_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CarD family transcriptional regulator"
                     /protein_id="HBH1521582.1"
                     /translation="MYKIGESVMYPKEGACCVNDIVTKKINHQMQKYYELSVIFNSNL
                     KISIPVLNADRIGIRPVMDGNDVDKFIQSIDKTDGVWVFDRKQRLKLYHDKFHSGDVF
                     EIVKLIKMLMIQDCSKQLCSTDKEFLNKAQRFALSELAAAQCKSYTVVLEEMKKHILN
                     SKSKN"
     gene            complement(5422..6486)
                     /locus_tag="KRL67_000678"
     CDS             complement(5422..6486)
                     /locus_tag="KRL67_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBH1521583.1"
                     /translation="MIRLTVANIKRYLKNHTLLINMVMLPIILIFSLNFFINNSENQS
                     MYFSPVAIVSDSSGKYEHKLINSSKLKENSFRLNEQDKAMNLLKNNKVSAVFVLDKNF
                     SNSIDNLQRPVVKCFKIENGGGSLWAESQIDSFITKSLKLKMDKNIDDKLTKTNIIDN
                     KPDKNKGSFLVVFLICYFMYINAAHLASDLFTLKKSNVLKRLISTDNKDIKIIFSIFL
                     GLFFIQSIVYTFALLCFSIIEDFNLSLNVLMIVLANSFVSTGFVFWIARVFKNESSIS
                     LISTFYSLIGLAISLSSLIPSMDKLSFMDNLSKLTPFYWTIDAIKNNGSILINIIILI
                     LIGIIFVTAGSLKLRDFAKN"
     gene            complement(6486..7586)
                     /locus_tag="KRL67_000679"
     CDS             complement(6486..7586)
                     /locus_tag="KRL67_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBH1521584.1"
                     /translation="MKLYMSVKTMLKGLKSAFILNLIYFLALPLILSWFLGMVTESMF
                     QNPIKTESTPIVIYDKDNTRLSNDLTKYLKNDLSYILTVKKDDSKAELKLTIPKGYES
                     SLLNEKSNTLNIEKLGTRDDIAVLLQDILDTYHEKFYLNNSQKISSEDFYKLFNKNSI
                     DTSIINNNVKQSSYEYFALVSLGFLVIIFIMNNILSNYISESKGLSKRLYSMPITRVQ
                     FLIYDFVGLWIYSFIFLLLYVLFFRILGITFKGNFAILLLLCALSSYFMTSISTFVTS
                     FFSKKYGTVIVYALLFLQTIFGGIFSMISDVFTKLTSLSPTYLIGELFSNYETFKTID
                     SIGNLIFTCLITSTILIALAIVKEKYKWREVQ"
     gene            complement(7600..8535)
                     /locus_tag="KRL67_000680"
     CDS             complement(7600..8535)
                     /locus_tag="KRL67_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBH1521585.1"
                     /translation="MDIVRVNNITKRFNDKLALDNISFSVKKGEIFGLIGPNGAGKST
                     LINIITNLMLPNSGSIQINGLDLSKDYIKAKSIIGLVPQELAIIETLTPFDNLEYFGA
                     FYGLKGKLLKERINEALEVTGLTEIKKKKVKKLSGGMQRRLNIGIALLNHPKILILDE
                     PTVGVDPQSRNHIFNFIKDISKKHETTVIYTSHYMEEVEHLCSKIFIMDEGKEIAFGD
                     NDYLKSLVSTNTKLILEIKNINAQLIFDLKNTKGVISVLENNSLLELDIAKKLQLTDI
                     LSIIDKNDSKIMKISYEEPSLEDVFLNLTGKNLRD"
     gene            complement(8664..9311)
                     /locus_tag="KRL67_000681"
     CDS             complement(8664..9311)
                     /locus_tag="KRL67_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBH1521586.1"
                     /translation="MNKIKVLIVDDEKLIRKGLKIILSSYNDLEIVGDASNGYEALEF
                     CKTNDVDIVLMDIRMKVCDGVLGTRLIKEYNNSITLLILTTFNDDEYIKDAMKFGASG
                     YLLKDSSDKVLHEGIRSSFFGNIVLDKSVAEKIMTSEKTIKQEYLYDMYNLTEKEISI
                     IRLIANGLNNKEISQELFLSEGTIKNNITNILAKLELRDRTQLAIFAFKNKIVIE"
     gene            complement(9304..10467)
                     /locus_tag="KRL67_000682"
     CDS             complement(9304..10467)
                     /locus_tag="KRL67_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HBH1521587.1"
                     /translation="MNRKKLVAFIRYLSLFILVVSYLKNVENTLYPVITMIILFLIII
                     NNQVRFFSLSNENKFILISYFLELILIFILCNYTKTFNSIYFVPLILDTSFLIKEKYK
                     YILFSFVIISSLIISLDKNIYLALESSSILLIITVLSIYIENENLSKLYSQNLYDKLR
                     ISKDELKKVNADLETYASSIEELAILKERNRISREIHDSVGHSLSTTIIQLNAIEKLL
                     KDKPLIYDLVHELREFVTESFQDVRRAISELKPVEYENYQSLFKIKELVKSFIKLTNI
                     NVKLTISKNTWNLSRNQSIALYRLIQESLSNSSRHGKATEIRIFITFNPSSFIITISD
                     NGIGCGNIKKGNGLNSISERIYELNGKVEFDNSNNGFTIRASFPKFSGGDFFE"
     gene            10651..10995
                     /locus_tag="KRL67_000683"
     CDS             10651..10995
                     /locus_tag="KRL67_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HBH1521588.1"
                     /translation="MNNKEKGDFGEKIAVNYLLSKGAKILEKNYRLKIGEIDIIAKMG
                     DEIIFVEVKSRSNIKFGYPCESVSFKKRKKIIGVANYYIIKNNLNNTPIRFDVIEVYL
                     LEKRINHIMNAF"
     gene            11026..12627
                     /locus_tag="KRL67_000684"
     CDS             11026..12627
                     /locus_tag="KRL67_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HBH1521589.1"
                     /translation="MLSIINSSNLVGIDSFLVKVEVDVSNGIPSFNIVGLPSKEIKEA
                     RERVKSAILNSGYKFPSTRIVVNLSPADIKKEGAFLDLSISIGLLRELIKKDENYIRE
                     SMFIGELSLDGKIRKVRGILPIIMGAKTQNIKRIFIPIENIKESLFVDEIDIIPIKSL
                     KECVDFLNEEIKVDKVRIMSVLNDKSRKENEELEKDNSYINCKYTKINNEESKYDEDF
                     KDVKGNYFVKRSAEIAAAGNHNMFMIGPPGSGKTMIAKRVRTILPDISIEEMIEVSKV
                     YSILGMINETKGIIDKRPFRAPHHTTTKQSLIGGGMDARPGEIALAHRGILFLDEIAE
                     FDRKILETLRQPIEDGYVNISRVKYSAKYPCRVLLVAAMNPCPCGYYMSETECRCRSN
                     EIDRYINKISGPLLDRFDIFVEVNSIKYSDFNSLKQEESSQKIKRRVENARKIQINRF
                     KKDNIKNNSEIKAYNLFKYCKLEKEASKTAEMIFNKYNLSSRSYTKLLKMARTIADLE
                     ERDLINSQCIIEAFSFRKAYYSYFK"
     gene            12646..13746
                     /gene="dprA"
                     /locus_tag="KRL67_000685"
     CDS             12646..13746
                     /gene="dprA"
                     /locus_tag="KRL67_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HBH1521590.1"
                     /translation="MEKRDTYLWLKSISGITTKTIEIIENEIVNIEDIFDFSEKEIYN
                     LKNISLNIRKNIVKYRGHAYLENIKELLYKKAIKYICKYDKEYPENLKNIYNAPKLLF
                     YKGDISLVNNNFNIAIVGSRKPTTYGINCAKTISCQLSQYGVNIVSGLAIGIDAYSHI
                     GCMSGKSKTIAVLGSGVDNPLPKQNLNLSNKILENGGLLLSEYNINSTVAPYHFSNRN
                     RIISGLSDGVVVVEAAIKSGALITVDFALEHGKNVFAIPGNINSQMSRGCHKIIKEGA
                     KLIENIDDILNEYNIFNIIDKKINQKYDNISLNAKSKQIIEAIKREGNLHIDSICDYT
                     GIEIKYVNSIINELVLNELVVEMNNKTYSLNV"
     gene            13779..15866
                     /gene="topA"
                     /locus_tag="KRL67_000686"
     CDS             13779..15866
                     /gene="topA"
                     /locus_tag="KRL67_000686"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HBH1521591.1"
                     /translation="MAKTLVIVESPAKAKTIEKFLGKSHYTVKASVGHVRDLPKSKLG
                     VDIENNFEPQYINIRGKGDVIKELKKEAKKSKQVYLATDPDREGEAISWHLAHILNLD
                     ESEDCRIEFNEITKDAIKKAIKHPRNIDINLVDAQQARRVLDRLLGYQISPILWQKVR
                     KGLSAGRVQSVTTKLICDREKEIKAFIPKEYWTIDVEAKTVKDKDITLKFYGKNDEKI
                     ELENEEAVNEILKEMEGKDLKVEKIESKSRRKSAPKPFTTSMLQQEGANKLFFTTKKT
                     MMIAQELYEGIDIEGEGTVGLISYIRTDSKRISDEAKEKAKGYIVDNLGENYYKNTGD
                     KKEKKDTKKVQDAHEAIRPTSVDRTPDSIKASLSKDQYKLYNLIWRRFVASQMEDSVF
                     DILNVECKIGDIVFKATGSKMKFDGYTKVYNFSEREDKILPSIEKGDILNIENILPDQ
                     HFTQPPARYTEASLVKTLEELGIGRPSTYAPTIATILNREYVEKQGTSLCPTELGIIV
                     TDILENNFHKFIDVDFTADMESKLDVIEEGNTDWKKIVSESYEPLKEAIEEALENIEK
                     VNMDEETDEICENCGSNMVIKYGRFGKFMACKNYPDCKTTRPIMNKIGVKCPKCEEGD
                     IILRKSKRGKAFYGCSNYPNCDFVAWNKPTGEFCEKCGSYMVEKVTKSETKTVCSNKE
                     CNKETKEHNDGKE"
     gene            16031..16816
                     /gene="codY"
                     /locus_tag="KRL67_000687"
     CDS             16031..16816
                     /gene="codY"
                     /locus_tag="KRL67_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-sensing pleiotropic transcriptional
                     regulator CodY"
                     /protein_id="HBH1521592.1"
                     /translation="MASEVLQKTRKINKTLQTSGGSSVSFDLLAGALGDVLSSNVYVV
                     SAKGKVLGLHLNDAQDSSVIEDEYTKQKKFSDEYTQNVLKIDETLENLNGEKILEIFP
                     EEHGRLQKYTTVVPILGSGQRLGTLVLSRYSNSFNDDDLVIAEYSATVVGLEILRAIG
                     EELEEEMRKKAVVQMAIGTLSYSELEAVEHIFAELDGKEGLLVASKIADRVGITRSVI
                     VNALRKFESAGVIESRSLGMKGTHIRILNDKLTDELKKLKNNQ"
     gene            16923..18188
                     /locus_tag="KRL67_000688"
     CDS             16923..18188
                     /locus_tag="KRL67_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HBH1521593.1"
                     /translation="MPLADKIRPKTMNDVIGQSHIIGNGKILSKILQSNFLPNMIFFG
                     PPGVGKTTVAEIIAERSNKSFYKINATNSSLEDIKKVISELGSINNVNGVLLYIDEIQ
                     SFNKKQQQSILEFMENGSITLIASTTENPYHYVYKALLSRSTVFEFKPLEKLDIEKGL
                     KRAVEVLNEDSYMDIECNNDAIEDIAILSDGDMRRALNILEVVVYSTKPNKDNITYID
                     SDIIRSSTFNKIINYDKNGDSHYDILSAFQKSIRGSDPQASIHYLARLIKGGDLISIC
                     RRLLVIASEDIGLAYPNAIVIVKACVDSAMQLGFPEAKIPLAEATILLATSPKSNSAC
                     IAIMKAMDELDKEFVKDIPNSIKDAHYSGSKEIGRGCGYKYPHNFKNHYVKQQYLPDS
                     IKDSIYYIPQENKTEKNIKKYLEYLNSDF"
     gene            18219..18989
                     /locus_tag="KRL67_000689"
     CDS             18219..18989
                     /locus_tag="KRL67_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBH1521594.1"
                     /translation="MIIKLNSTYHSRVMKYLKKEPEYNLFIIGDIERYGYGNNFLNIW
                     ADVGEHGEIKAILLKYFEFMMFYSDGEYDVEGFYNLLRNTNYEEISGKICAVDALAKR
                     LGLNNLKVVDFCKLQTKKFLIDNNCNAKVKRIRLGNLKKTVKLYDLIDEFHSTTLENL
                     KNGLRTGRGYCIEINKQVVSMAKSTSENRTHAMIIGVGTHPKYRAKGLATKCLIKLCS
                     ELLRENKIPCLFYDNEEAGKIYKKLGFENIGKWGIYSK"
     gene            19098..19544
                     /locus_tag="KRL67_000690"
     CDS             19098..19544
                     /locus_tag="KRL67_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HBH1521595.1"
                     /translation="MKLSTKGRYGLKAMFELALNQDNGPVSLKFIAKKQKISDQYLEQ
                     IFSSLKKSGLVKSVRGAQGGYLLSKNAEDITVGDILVVLEGPVALSDCVLDEDVCENS
                     NMCVTKIVWEKMKKGIEDVIDSITLKDMINDYNKNKLENDITNIKK"
     gene            19562..20755
                     /gene="nifS"
                     /locus_tag="KRL67_000691"
     CDS             19562..20755
                     /gene="nifS"
                     /locus_tag="KRL67_000691"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase NifS"
                     /protein_id="HBH1521596.1"
                     /translation="MEKRRLYMDYSATTPIKKEVLDAMMPYLTDYFGNASSFHTFGRE
                     AKDALDKAREQVAALINAEPSEIYFTAGGSESDNWTLEGVAYANKNKGNHIITSKIEH
                     HAILHTCEYLAKHHGFEITYLDVDSEGKVDLKQLEDSIKDTTILISIMFANNEIGTIQ
                     PIKEISEIAKKHKILFHTDAVQATGNIPVDVKELGIDLMSMSSHKIYGPKGVGALYIR
                     KGVRLHNFVHGGAQEKSKRAGTENIPAIVGYGKAAELAKENMQNHVETLTRLRNKLID
                     GVLERIPYTRVNGSLENRLPGNANFAFQFIEGEGILLLLDMLGIAGSSGSACTSGSLD
                     PSHVLLAIGLPHEIAHGSLRLTVGDFTTDDDIDYILENLPKVIERLRSMSPLYDDAKK
                     EGLVK"
     gene            20809..21249
                     /gene="nifU"
                     /locus_tag="KRL67_000692"
     CDS             20809..21249
                     /gene="nifU"
                     /locus_tag="KRL67_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold protein NifU"
                     /protein_id="HBH1521597.1"
                     /translation="MQYSDKVMEHFMNPRNMGEIDNASGVGEVGNPTCGDIMKIFLDI
                     DGDVIKDVKFKTFGCGSAIASSSMATEMIKGRTIKDALELTNKAVAEALDGLPPVKMH
                     CSVLAEQAVKAALIDYAQKNNIHIPELDGYVIDDAHDHDVEEEE"
     gene            21542..22621
                     /gene="mnmA"
                     /locus_tag="KRL67_000693"
     CDS             21542..22621
                     /gene="mnmA"
                     /locus_tag="KRL67_000693"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HBH1521598.1"
                     /translation="MNKKVMIGMSGGVDSSVAAYLLKQQGYDVIGVTMKLWQDDDDVE
                     IEGGCCSLSAVEDARRVANKIGIPFYVLNFREVFKEKVIDYFIDEYLEGKTPNPCIAC
                     NKHIKFDDFYKKARQIGCDYVATGHYAKIEKDESTGRYLLKKSVTDKKDQTYALYNLT
                     QEQLEHTLLPIGDYEKDRVREIAKEMGMAVHNKPDSQEICFVKDNDYANYVKKHSKKR
                     IEEGFFVDTKGNILGKHKGILYYTIGQRKGLGITFGKPMFVIDINPINNTIVLGDNED
                     LFKKELIAKDVNFISIDTLEEPLRVQAKIRYSAKPSPATIHRVGEDTIKIVFDEAQRA
                     ITKGQSVVMYDGDIVVGGGIIEKSL"
     misc_binding    22763..23001
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            23069..25708
                     /gene="alaS"
                     /locus_tag="KRL67_000694"
     CDS             23069..25708
                     /gene="alaS"
                     /locus_tag="KRL67_000694"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HBH1521599.1"
                     /translation="MEKMGLNEIRSKFLEFFESKGHYVANSYSLVPNNDKSLLLINSG
                     MAPLKNYFSGVEVPPSVRMCTSQKCIRTGDIENVGITARHATFFEMMGNFSFGDYFKR
                     ESIKWGWEFVTEWLNIPEDKIWVTVYEEDDDSYDIWAKEMNFPEERMVRLGKDDNFWE
                     IGTGPCGPCSEIYFDRGEEYGCDNPDCKPGCDCDRYLEFWNHVFTQFDRDEEGNYSLL
                     ENKNIDTGMGLERMGCIMQGVDTIFEVDTIKSILEAVEKLTGVKYGENPKNDISIRII
                     TDHIRAVTFLVSDGVLPSNEGRGYVLRRLLRRAARHGKLLGVKELFLQKLIDEVIKVN
                     DKAYPVLVEKESYIKKVVGIEEEKFNETIDQGTEILNSYIEVLKNEGKTVLSGQEAFK
                     LYDTYGFPIDLTKEILEEEHLSVDEEAFNEEMEKQKERARNARGNMDGESWKEDPLSK
                     LESTVDSTFNGYSEIYGEGTIEAIVKDDELVQSAKEGDKVSIVLDNTTFYPEGGGQVG
                     DCGLITNENLVLEVLNTKKGANNSIKHIGIIKSGRISNGDKVKTLVDGETRMSAARNH
                     SATHLLHKALREVLGEHVNQAGSLVTPERLRFDITHFEAISNEELKVIEEKVNNVILS
                     SLDIKCDIMNIKEAKEKGATALFGEKYGDEVRVVSMGDYSTELCGGTHLTNTSQVGMF
                     KILSEGGVAAGVRRIEAITGKAVYEYLKERDGIISEVCVNLKSKEDNLIQRISSLLEE
                     NKNLSKELHDMKAKMSLQSVDSIFDSKVEVNGVNLITNKFEGMDMDTLRETADNLRDK
                     LGSGVVVLANVVDDKVNFVVTATKDVLDKGIHSGNIVREVAKIAGGKGGGRPNMAQAG
                     ASDVSKVDQALSYASEVIKTQVK"
     gene            25733..25990
                     /locus_tag="KRL67_000695"
     CDS             25733..25990
                     /locus_tag="KRL67_000695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IreB family regulatory phosphoprotein"
                     /protein_id="HBH1521600.1"
                     /translation="MEKDLDYTMKFEGIPEDRMSVGDTIDFVYKALVEKGYNPINQII
                     GYLLSGDSSYITSHKNARAIIKKFERDEILEEVITHYLNRK"
     gene            26000..28171
                     /locus_tag="KRL67_000696"
     CDS             26000..28171
                     /locus_tag="KRL67_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH1521601.1"
                     /translation="MRKKIMSLFAIVIMIFVSTASYGFAEEKGKVIFIDMNRTNLGSM
                     MNIPILKNEIEKRGYVALMNIRGDQGTDDRRSYASMGAGTRASVTTEDYINFQKVDQD
                     TAKAFEVATGQKAKEINDLGINRSINENLEYGEYGANLGSLGKTLSENGLKTSVIGNA
                     DIIENNQLVRNRNLCLVAMDQYGRIDSGNIDNINKKDSKMPFGISTDYDKLLDETKKS
                     YKNSDAIFVELGDTYRLDMYRLNLNDNTYGTMKKNIVKNINKYLEQVFSMVGENDTVY
                     IASAFPSDLDYKNKRRLSPIIKFSGEGKGLLESATTRKAGIVANLDVGVDILGTFGLE
                     NKEMVGRSYTLVNKEDNISYLKSLYERIVSISSIRSTIINGFVGVISASWVIAMFAIF
                     FRNYMPHKERVFKVLKEFIKLGIIMPLSFMLAPLMNFKTPISISLGIFATTLILYLLG
                     RKLFKDNDIKHMGFFATLTILVIVIDSVFGTYLMQNNIMSYDAVIGARYYGVGNEYEG
                     VTIASAVFGLAVLLHYKKVSKLLVVIFSLIILITSAYPSMGANVGGAISECVAYLLFI
                     MLIYDIKIDFKKAVLLAVSAVVVVFAFAALDILSGSESHLGMFVQRIFLNGPGEILQT
                     FGRKIQMNMQLAQTSVWVNILLVGIGVIAVLIFKPSRHFRKIMNNFPILFKGFIASMV
                     GCIITLLVNDSGIVAASTASIYILIPLIIISINMIIFEKNN"
     gene            28377..28802
                     /gene="ruvX"
                     /locus_tag="KRL67_000697"
     CDS             28377..28802
                     /gene="ruvX"
                     /locus_tag="KRL67_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HBH1521602.1"
                     /translation="MLDGRIMGLDVGDKTIGVAVSDLMGLTAQGVKTIKRVGKKKDIE
                     ELKAIIKEKQVNKIVSGLPKNMNGTLGPQGEKVIKFCELVKAETGIDVEFWDERLSTV
                     AAERSLLEADVSRQKRKKVIDMLAAVIILQGYLDFKINS"
     gene            28880..29167
                     /locus_tag="KRL67_000698"
     CDS             28880..29167
                     /locus_tag="KRL67_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HBH1521603.1"
                     /translation="MEENIINLIDENGVESQFEIILTLEAEGKEYAILMPLDDEEAEE
                     ALIFRIDEDEEGEILIPLESDEEYETVVAVYTAIMEEEGLNYDEDESNGLN"
     gene            29449..29907
                     /locus_tag="KRL67_000699"
     CDS             29449..29907
                     /locus_tag="KRL67_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HBH1521604.1"
                     /translation="MANTMDLLKDKLKETGFKITPQRRAIVEILLKHDHSHLSSEEIY
                     DLVRVDCPEIGLATVYRTMQLLDEIGLISKLNLDDGCIRYEISLHKEDCHNHHHLICK
                     NCGKIMEAKEDLLDNIEKEIQSLYKFKILDHDVKFYGLCDECNGVSDSEE"
     gene            complement(30024..30761)
                     /locus_tag="KRL67_000700"
     CDS             complement(30024..30761)
                     /locus_tag="KRL67_000700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HBH1521605.1"
                     /translation="MRGKTHCAIGILTAIQTSLIFKIPISLVDILVSATFAVLPDLDK
                     SNSMVSNFILKNNVSKYIYRIFIYAVNIIIFFISININDNFYLSAIITFVAIIIIEAK
                     LTHTFLRKVFLSLIFILLAICLYIIEVEIYFTIFCLMLSIFPWLKHRSFSHSIFATII
                     VYFLLKQIELITNINNLSFYGTIGYASHMFLGDLFTKQGIPIFYPLSEKKISLGFLTV
                     GGPFSNFIEKSFIFVLIGLIIFSILKL"
     gene            30978..32654
                     /locus_tag="KRL67_000701"
     CDS             30978..32654
                     /locus_tag="KRL67_000701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HBH1521606.1"
                     /translation="MQLFKKNTNKIKVMALGGLNEVGKNMTVVEYKDEIIVIDAGLSF
                     PEDEMLGVDIVIPDITYLVKNRDKIKGIFITHGHEDHIGALPYILKKINVPVYGARLS
                     IGLIQVKLKEHKMNNVKLNVIGPRQVIKLDNMEVEFLKNNHSIPDAYSIAIHTDQGII
                     YHTGDFKIDLTPIDGDVMDMHRICELSKKGVLLMLADSTNAEKPGFTMSEKTVGVGLD
                     ELFAKGNGRRIIVATFASNIHRLQQIINTAEKFNRKVAISGRSMVNVVGVAKELGYLD
                     ISDDMLIDLNDICKYEDSELVIITTGSQGEPMSALARMAFSEHKKVEIKSGDLVIISA
                     HPIPGNEKLISRVINFLFEKGAEVVYSDIADIHVSGHACQEELKLIHALVRPKFFMPA
                     HGEYRMLKRHAEIAEQLGMDKENIFVMQTGDVLELDKNSAKVANRIQTGNILVDGLGV
                     GDVGNIVLRDRKHLSEDGLMIVVVTISKDEGKVLAGPDIISRGFVYVRESEDLMDGAK
                     DIIKNVLNECEEKNIKEWAYLKNNIKENLKEYLYQKTKRNPMILPIIMEV"
     gene            32862..33062
                     /locus_tag="KRL67_000702"
     CDS             32862..33062
                     /locus_tag="KRL67_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore protein"
                     /protein_id="HBH1521607.1"
                     /translation="MDDVSRQNAIKALKQTKMEIAGEYGMNYEDAFEIIENASNKGVL
                     EGYFKKLEKKKNLGQGISRHLE"
     gene            33116..34279
                     /locus_tag="KRL67_000703"
     CDS             33116..34279
                     /locus_tag="KRL67_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HBH1521608.1"
                     /translation="MKKLASTALAILIALTPLSFSFANNKENADANQLNISSKSAILM
                     DVGSGQILYEKDAHKKLPPASVTKVMTMLLIVEALDSGKIKLDDEVQVSETASSMGGS
                     QIFLEPGETQKVDTLLKGIAVASANDACVAMAEHLAGSVEGFVDRMNAKAKELNMNDT
                     HFANTNGLPVANHYTSAHDIALMSRELLKHEMISKYLTTWMDKVVVGKKQVTVGLANT
                     NKLIKHYQGATGVKTGFTQEAKYCLSASAKRGNTHLVAVTLGAETSPERFNDASSLLN
                     YGFANYESVKLCSKGDNIATLTLDKADENKVKLVAKEDLNALIKKGSSKEFEKKIEIV
                     KNPKMPIKKGTVLGKIKICKDKKVIGEVELINTKDINKASYLQMLQRIIDNMI"
     gene            complement(34475..34897)
                     /locus_tag="KRL67_000704"
     CDS             complement(34475..34897)
                     /locus_tag="KRL67_000704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2000 domain-containing protein"
                     /protein_id="HBH1521609.1"
                     /translation="METKNSKCVMVIDENLPMGIISNTAAIMGITLGKHAPETVGPDV
                     IDKTGNSHLGIIDIPVPILKGNKEIIKDLRKKLYTLEFNDLTVVDFSDVAQSCNLYEE
                     FTQKIASVPEDELQYFGIAIYGNKKKVNKLTGSMPLLR"
     gene            complement(34918..35754)
                     /locus_tag="KRL67_000705"
     CDS             complement(34918..35754)
                     /locus_tag="KRL67_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HBH1521610.1"
                     /translation="MKQEERTVCFDRELKIEAYRFKGIMQKFPNHFHEHYVIGFIEKG
                     QRYLSCKNKEYTTSTGDLLLFNPFDSHTCEQIDDKVLDYRCINIKPEIMKKTVFEITG
                     KNYLPKFNQPVIFRSELVPLLQELHYIIMEEELDFKKEELFFFLIEQLIEEHTEPNLQ
                     SNLENTNIEIQAVCDYLENNYAEHIVLDELSTIAGMNKYSLLRNFTKLKGITPYRYLE
                     NIRVNKAKKLLEKGVEPIDAAIQTGFVDQSHFTNFFKNFIGLTPKQYQNIFINDDSNN
                     FK"
     gene            36051..36647
                     /locus_tag="KRL67_000706"
     CDS             36051..36647
                     /locus_tag="KRL67_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HBH1521611.1"
                     /translation="MLSMNIGEIVASLVGIAMAISIHEFGHAYSAHLLGDDTAKAYGR
                     MTLNPVRHVDPIGLLAMFILRIGWAKPVPVNPNNFKNYKIGNLIVSLAGVFCNVLTAI
                     LCVIINKYVHMYAINTIAEYVFLYSIGFAAFNLLPIPPLDGWGVISTFVPYKWNEYVY
                     KFESMSYIILLIALFTGIYRIILTPIYAVLLKIVYLFV"
     gene            36677..37396
                     /locus_tag="KRL67_000707"
     CDS             36677..37396
                     /locus_tag="KRL67_000707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HBH1521612.1"
                     /translation="MKYNIQLQVYEGPLDLLYDLITKHKIDIKDISIIDITKQYLNYL
                     KMLDKMDLEITSEFITMASKLLEIKSKYLLYKQKDEEEDPRIELMEKLEEYRKFKVAS
                     QDIKENITYVNERFYRNKEEIIIDDNVDLEDISIESIKNILPYIFKVKTSQIENANDE
                     KLDKIVRKKIISVEEKILYIRDIIKDKIEVTFTNIIKSYENDEIIATFLSILELIKEK
                     EIVVVQDIFFDDILIKKSSEC"
     gene            37398..37937
                     /gene="scpB"
                     /locus_tag="KRL67_000708"
     CDS             37398..37937
                     /gene="scpB"
                     /locus_tag="KRL67_000708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HBH1521613.1"
                     /translation="MKREDIKYIIESVMFAYGEPISIKELNYIINKELSSKEIEIMLN
                     LLIEEYREQNRGIQIIKLENKYQMCTNKDYAEYIKKIIEPKKKKSLSQATLETLTIIA
                     YKQPITKVEIEDIRGVKCDKVLQTLFENELIREAGRLNKIGKPIIYKTTDEFLKLLNI
                     ESLEELPPIENYQEVATNE"
BASE COUNT        15198 a         4103 c         6573 g        12144 t
ORIGIN      
        1 aattaatatg aaaatataca aatatataaa aagtttgttt agagttatag ttaatgtgtg
       61 tattaattta atactaattt attggaggtc taaaaatgaa gtacataata aaaagagatg
      121 gtacaaaaga actttttagt aaaagtaaga ttgaagatgc tgtctataaa gctagtataa
      181 atagtgaagg tggagtagat agagatttgg catatgatat atcaagaaaa atagctaatg
      241 actataattc tatggaaaaa tgcttatccg tagaagaaat acaagatata gttgaagtac
      301 tacttatgga atcaaataga aaagatatag caaaacacta catattatat agggaaagac
      361 gctctgaaat aaggactaaa gcttgggagt ttgatgaatt acaaaaatca atatggaaga
      421 acaagtatag acataatggt gagagttttg atgaatggat aaagagaatt tcaaataata
      481 gttcaagaat atcaaaacta ataagacaaa aaagatttct atttgctgga agaattttag
      541 caaatagagg attacagaaa gaaggaaaaa aagtaacata ttccaattgt tatgtaatag
      601 aaccaccaaa agatagcata gaagatatat ttgatactgc aaaaaaactt gctagaactt
      661 ttagttatgg tggaggagtt ggaattgata tttcaaagct aagaccaaaa ggagcgaaag
      721 ttaacaatgc tgcaaaacat acgactggag cagtctcttt tatggaactt tacagtatga
      781 caacgggctt aattggtcaa agagggagaa gaggagctct tatgatttct atggatgtaa
      841 atcatccaga gatagaagag tttatagata taaaaacaga tttggagaag gttactaaag
      901 caaatatttc tgtaagagta acagatgagt ttatgcaagc tgtaagagaa aaaaagttat
      961 ataattgtac ttttgaagtt gaaactactg gagaaaaaat agttaagata gtgaatgctt
     1021 atgatttatt taataaattt gtgaaaaata actgggatta tgcagaacca ggtatattgt
     1081 tttgggataa aataaaaaca tataatttta tgagtgaaga taagaacttt tgcttttcag
     1141 gagtaaatcc ttgtgcagaa gagccacttc caagtggagg aagttgtcta cttggctcaa
     1201 taaacttgtc agagtttata atacaaccat ttggtgaaca tgctactttt gatattaata
     1261 aattaaaatc ttgcgttaga gaagctgtag tagctttaaa tgatgtctta gatgagggat
     1321 tgaacttgca cccactgcaa gaacaaagag atagtgtaca taagtataga cagataggtt
     1381 taggtgtaat gggtatagct gatatgctta taaaaatggg aataagatat ggttctgatg
     1441 aatctttaga actatgtgat ttaattggta agactatgtt aaatgaagct gtgaagcaat
     1501 cagcattaat tgctaaagaa tatggaacat ttagtgagta taaatatgaa aatatttcta
     1561 aatctaagtt ttttatagaa aatctaaatg aagacactaa ggaaatggtt aaaaagtatg
     1621 ggcttagaaa ttcacaactt ttaactatac ctcctacagg ttctatatct acaatgatag
     1681 gaattagtgg tggaatagaa cctatattta atctttcgta tataagaaaa actgaaagct
     1741 tgcatgatga agatgtgtat tataaagtat atactccaat tgttaaagag tatatggata
     1801 agaaaggtat tacagatgaa gaatgtttac cagatttttt tgttactgct atgaatatag
     1861 gatatgaaga aagaataaac atgcaaaaag tatggcaaaa gtatattgat gcttctatat
     1921 catctacaat aaatatacct tttgaaacta cagttgaaga tgttttcaat atatatatta
     1981 aggcatggga ggctggtctt aaaggagtaa caatatttag agatggatgt aaaaggagtg
     2041 gaattttaat aaatgaaaaa aaggaagaaa ataataaaga aaataaaaaa gaaaatagta
     2101 aaaaagaata ttataaaaat aataaaaaag aaaaagataa taaagataaa aaagccaaag
     2161 aaataataaa taaaaatttt gaagaagatg aaaaatttgt gtgccctgag tgtggaagtg
     2221 aaggaattgc acatacaggt ggatgtagta tatgtttaaa atgtggctat agtggatgca
     2281 attaaactct aattaaaaaa ctttaatata tttaaaactt aaatttttaa aaacgaaaga
     2341 tgagggaatg gtatgcagga taaaagtgtg agggagatta aagaaataat agagacttta
     2401 gaagtagaaa aatatatgga atatatagaa ctgttaagga ttgatgaaag aaaatctgta
     2461 caaggtttag ctataaaact agcaaaaaag ctggataata ttagaaaaga agaagagaga
     2521 ttggaaacta taaatatatt tgagaacgat ggctatgata aaggttattt atatattgga
     2581 ggaattgatg aggcgggaag agggccactt gctggacctg ttgtagcttc tgttgtagta
     2641 tttaaaaaag atacaaaaat agaaggtgta aatgactcta agaaactgag tgaggcaaaa
     2701 agagatgaac tttttgaagt aataaaagaa gaagcactag attatggaat aggtatagta
     2761 aacaatgaag aaatagatga atttaatata ttaaatgcta catatatggc aatgaaaaag
     2821 gctataaatt gtttaaaaaa agcaccagat tatttgttag ttgatgcagc aacaatacca
     2881 ggtattgata taactcaaaa tccaattgta aaaggagatt caaaatctat atcaatagca
     2941 gcagcaagta tacttgctaa agttactagg gatagtataa tgtaccaata tgatagagta
     3001 tacccagaat atgggtttaa atctcacaaa ggatatggaa ctaaagaaca ctatgaagct
     3061 attgaaaaat atggcataac tcctatccac agaaaaagtt ttttaaaaaa tatactgtaa
     3121 atataatatg atataatgta tataatataa atcaatatag ttaatggggg attaaagatg
     3181 gaatataatg aattattaaa aagtgctaga gaaaatttaa atggaagttg taaagtttgt
     3241 aaggtttgta atggggttgc ttgtgcaggt gaagtgcctg gcatgggagg taaaggaagt
     3301 ggttcttctt ttatagaaaa tagcaagagt ttagaaaaaa taaaagtaaa tatgagagta
     3361 atacatgatg tatcgaatcc tgatacatct atagagatgt ttggaaagaa aatgagtgca
     3421 cctatatttg cagctccagt aacgggaact actttaaata tgggtggaaa aattaatgaa
     3481 agagattaca tagaaccagt agttgcagga tgtgctaata gtggtattta tgcaatggtt
     3541 ggtgatacgg cagtagatgc ctttttaatg gaaaatttag atgtagtcaa aaagtataac
     3601 ggagctggaa ttgtatttat taaaccttgg gataatgaaa atattataaa aaagataaga
     3661 ttagctgaag aagctggtgc atttgcagtt ggagttgata tagacgcctg tggtctagta
     3721 actttatcat tacatggaaa accagtactt cctaaaaatg ttgaacaaat aaaggagtta
     3781 gtaaagtcta ctaaactacc atttatattg aaaggtataa tgactgttga ggatgcttta
     3841 atggcagtag aagcaggtgt ggatgcaata gttgtatcaa atcatggagg aagagtactt
     3901 gactgtacgc caggagcatg tgaagtttta ccaaaaattg ctgatgctgt taaaggtaaa
     3961 gttacaatac ttgcagatgg tggagtgaga actggtttag atgtactaaa aatgataggt
     4021 ttaggtgcag atgcagtatt aataggaaga ccttttgtga ctgcttcatt tggtggtgca
     4081 actgatggtg tagaaactta tgtaaataaa cttcaatcag agctaagtgg ctctatgata
     4141 ttaactggat gtcaaactat aaaagatatt gatggaaaag ttatatacaa ataaaatttt
     4201 atacaaaaaa gaggaaaaat cattttaatg tagaataatt gtacaaaggg ggaatttata
     4261 tgagtagaaa agcttatgaa gaagccttag tagaattaga aaaatttatt gatgaaagaa
     4321 aagaaataat taaaagtgcg gaggactgta ttgataagta catagttgac agaacgctac
     4381 cttttgatta caaagataaa tgtgtggaat ggcaacaaga gttacttgat attgcagagg
     4441 cacaagtttt agaagcaaat gaattaagtg tattattaca agagaaaaaa gaacttgaag
     4501 aagattaagt tttattagct aagagtaaac cagtaatatg atttactctt agttttttta
     4561 tttgaataaa gacatctatc taattggggt aactttatta atattagatt aggcagttta
     4621 aagtaagata gagtagcata aatgattagt ataaaaataa aaccagtctt taagactggt
     4681 ttaattttta cttttagaat ttagtatatg ttttttcatt tcttctaata ctactgtata
     4741 agatttacat tgagcagcag ccaattctga taaagcaaat ctttgagctt tatttaaaaa
     4801 ctccttatct gtactacaca attgttttga acagtcttgt atcataagca ttttaattaa
     4861 ttttacaatc tcaaaaacat cccctgaatg aaatttatca tggtacagtt taagcctttg
     4921 ttttctatca aaaacccata caccatctgt tttatcaata gattgaataa atttatcaac
     4981 gtcatttcca tccataactg gacgtatgcc aatcctgtct gcatttagaa ctggaatcga
     5041 aattttaagg ttactattga aaatgacact taattcatag tatttttgca tctgatgatt
     5101 tattttcttt gttacaatat catttacaca gcaggctccc tcttttggat acatcacact
     5161 ttcaccaatt ttatacatat attagttata cctccaaact gaattttaga tattgcaact
     5221 taggtacagt gttttaatta tctaataatt aatattatac cataaaaaac ttgacaagtc
     5281 aaaaatatta atatataata ttaagtttag attagatggt atataaaact aaatagaaaa
     5341 atataagata aaatgattta acatgttgga atattctttt atcattatat aaaaataaat
     5401 actaattata tttaataact actagttttt agcaaaatct cgtaatttaa gacttcctgc
     5461 agttacaaat ataattccaa tcaatatcag aattattata tttattaaga tactaccatt
     5521 gtttttaata gcatctatag tccaataaaa tggtgtaagt tttgaaagat tatccataaa
     5581 tgataattta tccattgatg gtattagact agatagagat atagccaaac ctattaaaga
     5641 ataaaatgta gatataagac ttatagaact ttcattttta aatactctag ctatccaaaa
     5701 tacaaatcca gtggaaacaa aactatttgc tagtacaatc atcaatacat tcagactcaa
     5761 attaaagtct tctataattg aaaaacataa tagagcaaaa gtgtatacaa ttgattgaat
     5821 aaaaaataac cctaaaaata tactgaaaat tatttttata tctttattat ctgtagagat
     5881 aagtcttttt agcacatttg atttttttaa tgtaaataaa tcagaagcaa gatgtgccgc
     5941 attaatatac ataaaatagc atatcaagaa gactactaaa aatgaacctt tattcttatc
     6001 tggtttgtta tcaattatat ttgtctttgt caatttatca tctatatttt tatccatttt
     6061 caattttaac gatttagtaa taaaagagtc tatttgtgat tctgcccaaa gactaccacc
     6121 accattttca attttaaagc atttaactac aggtcgttgt aagttatcta tactatttga
     6181 aaaattttta tctaagacga atacagcaga aaccttatta ttttttaata gattcatagc
     6241 cttatcttgt tcatttaatc taaatgaatt ttcttttaac ttagaactat ttattaactt
     6301 atgttcatac tttcctgaag agtcagatac aattgctact ggagaaaagt acattgattg
     6361 attttccgag ttgttgataa aaaaattaag tgaaaaaatt agtataatag gaagcataac
     6421 catattaatt aaaagagtat gattttttag atatcttttt atatttgcaa cagttaatct
     6481 aatcattatt gaacctccct ccatttatat ttttccttta caatagcaag tgcaatcaat
     6541 attgtagaag taattaaaca tgtgaaaatt aaatttccta ttgaatctat agttttaaat
     6601 gtttcataat tactaaacaa ttctcctatt aaataggtag gagataaact tgtaagctta
     6661 gtaaatacat cacttatcat actaaagata cctccaaaaa ttgtctgtaa aaagagtaat
     6721 gcataaacta ttactgtacc atatttttta ctaaaaaagc tggttacaaa ggtagatatt
     6781 gaagtcataa aataagatga taaagcacaa agtaggagca atatggcaaa attaccttta
     6841 aatgttattc caagtattct aaagaataaa acatataata ataagaatat aaatgaataa
     6901 atccataaac ctacaaagtc ataaattaag aattgtactc tagttatagg catagaataa
     6961 agtctttttg aaagtccctt tgattcactt atataatttg ataaaatatt gttcattata
     7021 aaaattatta caagaaatcc tagcgaaact aaagcaaagt attcataaga gctctgttta
     7081 acattgttat ttattataga agtatcaata gaatttttat taaataattt atagaaatct
     7141 tctgaactta ttttctggga attatttaaa taaaattttt catggtaagt atctaatatg
     7201 tcttgaagca atactgcaat atcatcacga gttccaagtt tttctatatt tagagtattt
     7261 gatttttcat ttaataaact actctcatag cctttaggta tagtaagctt taattctgcc
     7321 ttagaatcat cttttttaac tgttaatata taagataaat catttttcaa atactttgtc
     7381 aaatcatttg ataatcttgt gttgtctttg tcatatataa caatgggtgt agattctgtt
     7441 tttatagggt tttgaaacat gctttctgta accataccaa gaaaccaaga taaaatcaga
     7501 ggtaaagcta aaaaatatat taaattaagt ataaaggcgc tttttaaacc ctttagcata
     7561 gtttttacac tcatgtataa tttcataaaa tcacctccat tagtccctta aatttttgcc
     7621 tgttagattt aaaaatacat cttctagact aggttcttca taagaaattt tcattatttt
     7681 tgaatcgttt ttgtcaatta tagagagtat atctgtaagt tgtaattttt tagctatatc
     7741 caattctaat aatgaattat tttctaaaac agatataaca cctttagtat tttttaaatc
     7801 aaagataagt tgagcattta tattttttat ttccagaatt aattttgtat ttgtagatac
     7861 tagtgatttt aaataatcat tatctccaaa agcaatttct ttaccttcat ccataataaa
     7921 aattttagaa caaaggtgtt caacttcttc catataatgg gaagtatata ttacagtagt
     7981 ttcatgtttt ttagatatat cttttataaa attgaaaata tgatttcttg attgggggtc
     8041 tacacctaca gttggttcat ccaatattaa tattttagga tgatttaaga gtgctattcc
     8101 aatatttaat ctcctttgca taccaccaga taattttttg acctttttct ttttgatttc
     8161 agtaagacct gtaacttcta atgcttcatt gattctttct ttaagtagtt ttccttttaa
     8221 accatagaaa gctccaaagt actctaagtt atcaaaagga gtaagagttt ctataattgc
     8281 taattcttgt ggaactaatc ctattataga ctttgctttt atataatctt ttgataaatc
     8341 caaaccattg atttgaatac taccactatt tggtaacata agatttgtaa ttatatttat
     8401 aagtgtagat ttacctgcac catttggacc aataagtcca aatatctcgc cttttttaac
     8461 agaaaagctg atgttatcaa gtgctaattt atcattaaat ctttttgtta tattatttac
     8521 tctgactata tccataataa tccctccaaa ataattttat ataagttaaa aagctataat
     8581 actataataa agaattgtga attataattc tattactata agtcactaaa aaaagtgact
     8641 taagtcactt tatattttta gatttattct attacaattt tatttttgaa agcaaagatt
     8701 gctagttgag ttctgtcacg aagttctagt ttagctaaaa tattagttat gttattcttt
     8761 attgtacctt cagataagaa tagttcttga cttatttctt tattattaag tccatttgca
     8821 attagtctaa ttatagatat ttctttttct gttaaattgt acatgtcata aagatactct
     8881 tgtttaatcg ttttttcaga ggtcatgatt ttttcagcta cacttttatc aagcacaata
     8941 ttgccaaaaa atgaagagcg aatcccttca tgcaatactt tatcagagct atcttttaaa
     9001 aggtatcctg aagcaccaaa cttcatagca tcttttatat attcatcatc attaaaagtt
     9061 gttaaaatta ataatgttat agaattatta tattccttta taagccttgt acctagaacc
     9121 ccatcacata ctttcattcg tatatccata agaactatgt ctacatcatt cgttttacaa
     9181 aattctaaag cttcatatcc attagaagca tctcctacaa tttctaaatc attataagat
     9241 gataatataa ttttaagacc ttttctaatt aatttttcat catctactat aagaacttta
     9301 attttattca aaaaaatcac cccctgaaaa tttaggaaaa gaagctctaa ttgtgaaacc
     9361 attattagaa ttatcaaatt caactttacc atttaattca tatattcttt cactgatact
     9421 attaagtcca tttccttttt ttatattgcc acatcctata ccattatcac taatagtaat
     9481 tataaaactt gaagggttga atgtaatgaa aattctgatt tcagttgcct ttccatgcct
     9541 agatgagttg gaaagggatt cctgtataag tctatataaa gctatagatt gatttctaga
     9601 taagttccaa gtattttttg aaatagttaa tttaacatta atatttgtaa gcttaataaa
     9661 gcttttaact aattctttga ttttaaataa actttggtaa ttttcatatt caacaggttt
     9721 taactcagat atagcccttc ttacatcttg aaaactttcg gttacaaatt ctctaagttc
     9781 atgtactaag tcataaatta gaggtttgtc ttttaagagt ttttctatag catttaattg
     9841 tattattgtt gtagaaagag aatgtcctac actatcatgt atttctcttg atattcgatt
     9901 tctttctttt aaaattgcta attcttctat agaacttgca taagtttcca aatcagcatt
     9961 aacttttttt aattcatctt tagaaattct aagcttatca taaagatttt gactgtataa
    10021 ttttgataaa ttctcatttt ctatatatat agaaagtaca gttataatta gcaatataga
    10081 acttgactcc agtgccaaat atatattttt atctaaacta ataataagag aagatattat
    10141 aacaaaagag aacaatatat acttatattt ttcctttatt aaaaatgatg tatctaatat
    10201 taaaggaaca aagtaaatcg aattaaaagt ttttgtataa ttacaaagta taaaaattaa
    10261 tataagttct aaaaagtaag aaataagtat aaatttattc tcatttgata aagaaaaaaa
    10321 tctaacttga ttatttatta taattagaaa taatattatc atagttatga ccggatacaa
    10381 tgtgttttca acatttttca aatagcttac aactaatata aataaagata aatatcgtat
    10441 aaaagcaact aatttttttc tgttcattaa aggcactcct ccaaaaaaat aaacttttcc
    10501 tatgaaaact agaataatat cataaaagaa taaagtcaat agaagtattt attaatattg
    10561 atggaaataa aatcaattca aaaaattgaa tagtaaaatt gtatactgac cataatttaa
    10621 aagtgaaaat ttaatgtatt ggagtgaata atgaataata aagaaaaagg tgattttgga
    10681 gaaaaaatag cagttaatta tttgctatca aaaggagcaa agatactaga aaaaaattac
    10741 agattaaaga taggggaaat agatataata gcaaaaatgg gagatgaaat tatatttgtg
    10801 gaggtgaaat caaggtctaa cataaagttt ggctaccctt gtgaatctgt aagcttcaaa
    10861 aaaagaaaaa agataattgg agttgcaaat tattatatta taaaaaataa cttaaataac
    10921 acccccataa gatttgatgt tatagaggta tatttattag aaaaaagaat aaatcacata
    10981 atgaatgctt tttaaaaaag ataatgtttt tggaaaggat taaatatgtt aagtattatt
    11041 aattcaagca acttagtagg aatcgacagt tttttagtta aggttgaagt agatgtaagc
    11101 aatggaatac ctagttttaa tattgttggt cttccaagca aagaaattaa agaagcaaga
    11161 gaaagagtaa agtcagcaat tcttaatagt ggatataaat ttccaagcac gagaatagtt
    11221 gtaaatttat caccagcaga tataaaaaaa gaaggagctt ttttagattt atctataagt
    11281 ataggtcttt taagagagtt aataaaaaaa gatgaaaatt acataagaga aagtatgttt
    11341 ataggagaat tatcattaga tggaaaaata agaaaagtaa gaggtatatt gcccataatt
    11401 atgggtgcaa aaactcagaa cataaaacgt atttttatac caattgagaa cataaaagaa
    11461 agtctatttg tagatgaaat tgatataatt cctattaaaa gtttaaaaga atgtgtcgat
    11521 tttttaaacg aagaaataaa agtcgataaa gtaaggataa tgtctgtttt gaatgataag
    11581 tcaagaaaag agaacgaaga gttagaaaaa gataatagtt atataaattg caaatatact
    11641 aagattaata atgaagaaag taaatatgat gaagacttta aagatgtaaa aggaaattat
    11701 tttgtaaaga ggagtgcaga aattgcagca gctggaaatc ataatatgtt tatgattgga
    11761 cctccaggct caggaaaaac tatgattgca aaaagagtaa ggacaatttt accagatata
    11821 agtatagaag aaatgataga agtaagtaag gtttatagta tattgggtat gataaatgaa
    11881 actaaaggaa taattgataa aagacctttt agagcacctc atcatacaac tacaaagcaa
    11941 tctttaattg gaggaggaat ggatgcaaga cctggagaaa ttgctttagc acatagaggt
    12001 atcttatttt tagatgagat agccgaattt gatagaaaaa tacttgagac tttaagacaa
    12061 cctatagaag atggatatgt aaatatatcc agagtaaagt acagtgcaaa atatccttgt
    12121 agagtattgt tagttgcagc tatgaatcca tgtccatgtg gttattatat gtcagagaca
    12181 gagtgtagat gtagaagtaa cgaaattgat agatacataa ataaaatatc aggtccattg
    12241 ttagataggt ttgatatttt tgtagaagtt aattctataa aatattcaga ttttaattct
    12301 ttaaaacaag aagaaagctc tcaaaaaata aagagaagag tagaaaatgc tagaaaaata
    12361 cagataaata gatttaagaa ggataatata aaaaataata gtgaaattaa agcttacaat
    12421 ttatttaaat attgtaaact ggaaaaagaa gcttcaaaaa cggctgagat gatatttaat
    12481 aaatacaact taagcagtag aagttataca aaacttttaa aaatggcaag aacaatagcg
    12541 gatttagaag aaagagactt gataaattca cagtgtatta tagaagcttt ttcatttaga
    12601 aaggcttatt atagttattt taaatagttt tttgaggtgg attttatgga aaaaagagat
    12661 acttatttat ggttaaaatc aattagtgga ataacaacaa aaacaattga aataatagaa
    12721 aatgaaatag taaatattga agatatattt gatttttctg aaaaagaaat atataattta
    12781 aaaaatataa gcttgaatat tagaaaaaat atagtaaaat atagaggtca cgcttattta
    12841 gaaaatataa aagaactact ttacaaaaaa gcgattaaat atatttgtaa atatgataaa
    12901 gaatatcctg aaaatttaaa gaatatatac aacgctccaa agttattatt ctacaaaggt
    12961 gacataagtt tagttaataa taattttaat atagctatag taggttcaag aaaacctaca
    13021 acttatggaa ttaattgtgc aaaaacaata agttgtcaac tatcccaata tggagttaat
    13081 atagtaagtg gattagctat aggaatagat gcatactcgc acataggttg tatgagtgga
    13141 aaatctaaaa ctatagcagt acttggttca ggagtagaca atcctcttcc aaaacaaaat
    13201 ttaaatttat caaataaaat attagaaaat gggggattat tattatcaga gtataatatt
    13261 aactcgacag tagccccata ccatttttct aatagaaata ggattataag tggtttaagt
    13321 gatggagtag ttgttgtaga agcagcaata aaaagtggtg cactaatcac agtagatttt
    13381 gctcttgaac atggaaaaaa tgtttttgca atacctggta atataaattc tcaaatgagt
    13441 agaggttgtc ataaaattat aaaagaaggt gcaaaattaa ttgaaaatat agatgatatt
    13501 ttaaatgaat ataacatatt taatattatt gataaaaaaa taaatcaaaa atatgataat
    13561 ataagtctaa atgccaagtc taagcaaata attgaagcta taaaaagaga aggaaacttg
    13621 catatagata gtatttgtga ttatactggt atagaaataa agtatgtaaa ttcaataatc
    13681 aatgaattag tactaaatga gttagtagta gaaatgaata ataaaacgta cagtttaaat
    13741 gtataaatta agattaggta gtgggggtgt atggttttat ggccaaaaca ttagtcatcg
    13801 tagaatcgcc tgcaaaagct aaaactatag aaaaattttt agggaaaagt cattacacag
    13861 ttaaagcatc agttggtcat gtcagagatt tacctaaaag taaattaggt gtagatatag
    13921 aaaataattt tgaacctcaa tatataaata taagaggaaa aggcgatgta ataaaagaat
    13981 taaaaaaaga agctaaaaaa tctaaacaag tgtatctagc aactgaccct gatagagaag
    14041 gtgaagctat atcatggcat ttagctcata tactaaattt agatgaatct gaagattgta
    14101 gaatagagtt taatgagata actaaggatg caattaaaaa agcaattaaa catcctagaa
    14161 atattgatat aaatttggtt gatgcacaac aggcaagaag agtgctagac agacttttag
    14221 gttatcaaat aagtcctata ttatggcaaa aggttagaaa aggtcttagt gcaggtagag
    14281 tgcaatctgt tacaacaaag ttaatttgtg acagagaaaa agaaataaag gcatttattc
    14341 caaaagaata ttggacaata gatgttgagg ctaagacagt aaaagacaaa gatataactt
    14401 tgaaatttta tggtaaaaat gatgaaaaga ttgaattaga aaatgaagaa gctgtaaatg
    14461 aaatactaaa agaaatggaa ggtaaagatt taaaggttga gaagatagaa tctaaatcaa
    14521 gacgtaaatc agctccaaaa ccatttacaa caagtatgct tcaacaagaa ggagcaaata
    14581 agttattttt tactactaaa aaaacaatga tgatagcaca agaattatat gaaggtatcg
    14641 acatagaagg tgaaggtact gttggtctta tatcatatat aagaactgac tctaaaagaa
    14701 tatctgatga agcaaaagaa aaagctaagg gttatatcgt agacaattta ggagaaaact
    14761 actataaaaa cactggtgat aaaaaagaaa aaaaggatac taaaaaggtt caagatgccc
    14821 atgaggcaat aagaccaacc tcagttgata gaactccaga tagtataaaa gcctcattga
    14881 gcaaagacca gtacaagtta tacaatttaa tttggagaag atttgtagct agtcaaatgg
    14941 aagattcagt gtttgatata ctaaatgtag aatgtaaaat aggcgatatt gtatttaagg
    15001 caactggttc aaagatgaaa tttgatggtt ataccaaggt atataatttt tcagaaagag
    15061 aagacaaaat tttaccaagt atagaaaaag gagatatatt aaatatagaa aatatattgc
    15121 cagaccaaca ttttacacaa ccaccagcaa gatatacaga agctagttta gtaaaaacac
    15181 tagaagaact aggtataggt agaccaagta cgtatgctcc aacaatagct actatactga
    15241 atagagagta tgttgaaaag caaggaacaa gtctatgtcc aacagaactt ggaataatag
    15301 ttactgatat tttggaaaat aatttccata aatttataga tgtggatttt actgctgata
    15361 tggaaagcaa acttgatgtt atagaagaag gtaatacaga ttggaaaaaa attgtatctg
    15421 aatcatatga gccgcttaaa gaggctattg aagaagcact tgaaaatata gaaaaagtaa
    15481 atatggatga agaaactgat gagatttgcg agaactgtgg ttctaatatg gttatcaaat
    15541 atggtagatt tggaaagttt atggcttgta aaaactatcc cgattgtaaa acaactagac
    15601 caattatgaa taaaattggt gttaaatgtc caaaatgtga agaaggagat attatactta
    15661 gaaaatctaa aagaggtaaa gctttttatg ggtgttctaa ttatccaaac tgtgactttg
    15721 tagcttggaa taagccaaca ggagagtttt gtgagaagtg tggttcatat atggttgaaa
    15781 aagtgactaa atctgaaaca aaaacagtgt gttctaataa agaatgtaat aaagagacaa
    15841 aagagcataa tgacggaaaa gagtgaaaat tgaaaaaaat cacataattc tcttgaaaaa
    15901 ggttccaatc tatgataatc tttcaagtgt aaacaactaa tagaaaaata aattttcatt
    15961 aaatgtgaaa ataatattag aaaatataaa aggttattaa aaggcctaaa tttcatgagg
    16021 agatgattaa atggcaagtg aagtgttaca aaaaacaagg aaaataaata aaacattaca
    16081 aacaagtggt ggaagcagtg tctcttttga tttactggcc ggagcattgg gcgacgtttt
    16141 aagttctaat gtttatgtag taagtgcaaa aggtaaagta ctaggtcttc atttaaatga
    16201 tgctcaagac agttcagtta tagaagatga gtatactaag caaaagaaat tttcagatga
    16261 atatactcaa aatgtgttaa aaatcgatga aacattagaa aatttaaatg gtgagaagat
    16321 attagaaatc tttcctgaag aacatggaag attacaaaaa tatactacag tagttccaat
    16381 attaggaagc ggtcaaagat taggaacatt agtactttca agatattcaa attcattcaa
    16441 tgatgatgat ttagtaatag ctgaatacag tgcaactgtt gttggtcttg aaatattaag
    16501 agcaataggt gaagaattag aagaagaaat gagaaagaaa gctgtagttc aaatggcaat
    16561 aggcactctg tcctactccg agcttgaagc agttgaacat atttttgctg aattggatgg
    16621 aaaagaaggt ctacttgtag caagtaagat agctgataga gttggtataa ctaggtctgt
    16681 aatagtaaat gcacttagaa aatttgagag tgcaggtgtg atagaatcaa gatcattagg
    16741 tatgaaaggt actcatataa gaatacttaa tgacaaactt acagatgaat taaaaaaatt
    16801 aaaaaacaat caataatcta tattttatag gtttagatta gaaaaggtat ctatttcaaa
    16861 tagatacctt ttttagacga attattaaga taatgataag tatattaaaa aggagtgaga
    16921 aaatgccttt agcagataaa ataagaccaa aaacaatgaa tgatgtaata ggtcaaagtc
    16981 atataatagg taatggaaaa atattgtcta agatattaca atctaatttt ttaccaaata
    17041 tgatattttt tggtcctcct ggtgttggta aaactacagt agcggaaata atagcagaaa
    17101 gaagcaataa aagcttctat aaaataaatg ctactaattc atctttagaa gatataaaaa
    17161 aggtgatatc agagcttgga agtataaata atgtaaatgg agtcctatta tatatagatg
    17221 aaatacaaag ttttaataaa aaacagcaac aatcaatatt agaatttatg gaaaatggga
    17281 gtataacttt gattgctagc actacagaaa atccatatca ttatgtatat aaagctcttt
    17341 taagtaggtc tactgtattt gaatttaaac ctttagaaaa acttgatata gaaaaaggac
    17401 tgaaaagagc tgttgaagta ttaaatgaag atagctatat ggatattgag tgtaataatg
    17461 atgctattga agatatagca attctttctg atggagatat gagaagagcc ttaaatattt
    17521 tggaagtggt agtctactca actaaaccaa ataaggataa tataacttat atagattcag
    17581 atataataag gtcatctaca tttaataaaa ttataaacta tgacaaaaat ggtgatagtc
    17641 attatgatat tttaagtgca tttcaaaaat ctataagagg aagtgaccca caggcatcta
    17701 tacactatct agcaagactt ataaaaggtg gggatttgat aagtatatgt aggcgacttt
    17761 tagtaatagc tagtgaagat ataggtttag cttatccaaa tgctattgta attgtaaaag
    17821 catgtgtaga ctcagctatg caacttggat ttccagaagc gaaaatacca ttagcagaag
    17881 ctactattct tttggctaca tctccaaaat ctaattctgc atgtatagca ataatgaaag
    17941 ctatggatga actggataaa gagtttgtta aggatatacc aaatagcata aaggatgctc
    18001 actattctgg ttctaaagaa ataggtaggg gttgtggata caaatatcct cataatttta
    18061 aaaatcatta tgtaaagcaa cagtatttac cagatagcat aaaagatagt atttattaca
    18121 taccacaaga aaataaaact gagaaaaata taaaaaaata tttagaatat ctaaatagtg
    18181 atttttagac tgaactttat gtatcggggg tagatattat gattataaaa cttaactcaa
    18241 cttatcacag tagagttatg aaatacttaa aaaaagaacc tgagtacaat ttgtttataa
    18301 taggggatat tgaaagatat ggatatggaa ataatttttt aaatatatgg gcagatgtag
    18361 gtgaacatgg tgaaataaag gcaatattac ttaaatattt tgaatttatg atgttttatt
    18421 ctgatggaga gtatgatgtt gaaggttttt ataatctact tcgtaatact aattacgaag
    18481 aaatatctgg gaaaatatgt gctgtagatg ctttagcaaa gagattagga cttaataatt
    18541 taaaggtagt tgatttttgc aaacttcaaa ctaaaaagtt tttgatagat aataattgta
    18601 atgcaaaggt taagagaatt aggcttggaa atctaaaaaa gacagtgaaa ctttatgact
    18661 taatagatga atttcacagt acaactttag agaatttaaa aaatggacta aggacaggta
    18721 gaggatattg tatagagatt aacaaacagg tggtgtcaat ggcaaagagt acttcagaaa
    18781 atagaactca tgcaatgata attggagtag gaacacatcc taaatataga gctaaaggac
    18841 ttgctacaaa atgcctaatt aaactatgtt cagagttact gagagaaaac aaaattcctt
    18901 gtttgtttta tgataatgaa gaggctggaa agatatataa aaaactagga tttgaaaaca
    18961 ttggaaaatg gggaatttat tcaaaataaa aaattaaaat taatcttgac aaaattagta
    19021 gggtataata aaattatctt gaaagtaatt cctactaaaa cagtatgaat tagatttcta
    19081 tatagaggtg ataatatatg aagttgtcta ctaaaggtag atatggacta aaagcaatgt
    19141 ttgaattagc attaaatcaa gacaatggac cagtatcatt aaaatttatt gctaagaagc
    19201 aaaaaatatc agatcagtac ttagaacaaa tattttcatc tttaaaaaaa tcgggtctag
    19261 taaaaagtgt gagaggtgct caaggaggtt atttgctttc aaagaatgct gaagacataa
    19321 cagtaggaga tatacttgta gtattagaag gaccagttgc attatctgac tgtgtactag
    19381 atgaagatgt atgtgaaaac tcaaacatgt gtgtaacaaa aatagtttgg gaaaagatga
    19441 aaaagggtat agaagatgtt atagattcaa ttactcttaa agatatgata aatgactata
    19501 acaaaaataa attagaaaat gatataacaa atattaaaaa atagatttgg agatgaatgt
    19561 aatggaaaaa agaagattgt atatggatta ttctgcaaca acacctatta aaaaagaagt
    19621 attggatgca atgatgccat acctaacaga ttattttggt aatgcatcta gttttcacac
    19681 attcggaaga gaagcaaaag atgctttgga taaagcaaga gaacaagtag cagcccttat
    19741 aaatgctgaa cctagtgaaa tatattttac agcaggtggt tcagaaagtg ataactggac
    19801 actagagggt gtagcttatg ctaataaaaa caaaggtaat catataataa cttcaaaaat
    19861 agagcaccat gcaatacttc atacatgtga atacttagca aaacatcatg gatttgaaat
    19921 aacttattta gatgttgata gcgaaggaaa agtagacttg aaacaactag aagattcaat
    19981 aaaagatact acgatactta taagcataat gtttgcaaat aatgaaatag gaactataca
    20041 gcctattaaa gaaatatccg aaatagctaa aaaacataag atattattcc acactgatgc
    20101 agtacaagct acaggtaata ttccagtaga tgttaaagaa ttaggaatag atttaatgag
    20161 tatgtcttca cataaaatat atggaccaaa aggtgtagga gctttataca taagaaaagg
    20221 tgtaagactt cataactttg tccatggtgg agcacaagaa aaaagcaaaa gagctggtac
    20281 agaaaatatc cctgcaatag ttggatatgg aaaagcagca gaattagcta aggaaaatat
    20341 gcaaaatcat gtagaaactt taactcgtct tagaaataag ttaatagatg gtgtgttaga
    20401 aagaatacct tatacaagag taaatggtag cttagaaaat agattaccag gaaatgcaaa
    20461 ctttgcattc caatttatag aaggtgaagg aatactttta ttattagata tgttaggaat
    20521 tgctggttca agtggttctg catgcacttc tggttcatta gacccttcac atgtacttct
    20581 tgcaataggt ttaccacatg aaatagcaca tggttcatta agacttacag ttggagattt
    20641 tacaacagat gatgatatag attatatact agagaactta ccaaaagtaa tagaaagact
    20701 tagaagcatg tcaccacttt atgatgatgc aaagaaagaa ggtttagtaa aatagacaga
    20761 aagataaagc ataaaaaata gacataaatt gagtaggagg aaaagataat gcaatatagt
    20821 gataaagtta tggaacattt tatgaatcca agaaatatgg gagaaattga taatgcaagt
    20881 ggtgtaggtg aagttggaaa ccctacatgt ggagatataa tgaagatatt tttagacata
    20941 gatggagatg taataaagga tgttaagttt aaaacatttg gctgtggttc agctatagca
    21001 agttcttcta tggcaactga aatgataaaa ggaagaacta taaaagatgc attggagctt
    21061 acaaataaag ctgttgcaga agctttggat ggtttaccac cagtaaagat gcattgttca
    21121 gttttagctg aacaagctgt aaaagcagct cttatagatt atgctcaaaa gaacaatatc
    21181 catataccag aattggatgg atatgttata gatgatgctc atgaccatga tgttgaagaa
    21241 gaagaataaa aataagtgat tgtgtgtgtt atgagaagtt tacaatgatt aaaaagaagt
    21301 actaatgata taattaggac ataaaaaata gctgttttta aaatggctat tttttattgc
    21361 ctaaattaag tattttaact tgatttagag gttaattagt taacaaaata atccataaga
    21421 ttcaaataat aaagatgatt atttttttag aaaatagaac aagctatatt ttaaataagt
    21481 aaaatgcttg ctctatatgg tataataata agtaatgcaa aagatgaggt gaaatttgtt
    21541 tatgaataaa aaagtaatga taggtatgag tggaggagtt gatagctctg tagctgcata
    21601 tcttttaaaa caacaaggat atgatgtaat tggtgttact atgaagttgt ggcaagatga
    21661 tgacgatgtt gaaattgaag gtggttgttg ctctttatct gctgttgaag atgctagaag
    21721 ggtagcaaat aaaataggta taccatttta tgttttaaat tttagagaag tgtttaaaga
    21781 aaaagtaata gattatttta tagatgaata tttggaagga aaaactccta atccctgtat
    21841 agcttgtaat aaacatataa aatttgatga tttttataag aaggctagac aaataggttg
    21901 tgattatgta gctacaggtc actatgcaaa gatagaaaag gatgaaagta caggaagata
    21961 tttgctaaaa aaatctgtta cagataaaaa agaccaaact tatgcacttt ataatcttac
    22021 acaagagcaa ctagagcata ctttattacc aataggtgac tatgaaaaag atagagtaag
    22081 agaaattgct aaggaaatgg gtatggcagt acacaataag cctgatagtc aagaaatttg
    22141 ttttgtcaaa gataatgact atgcaaatta tgtaaaaaaa cattctaaaa agcgtattga
    22201 agaaggtttt tttgtagata ctaaaggcaa tatacttgga aaacataagg gtatattata
    22261 ttatactata ggtcaaagaa aagggcttgg aattactttt ggaaaaccaa tgtttgtaat
    22321 agacataaat ccaataaata ataccatagt tcttggagat aatgaagatt tatttaaaaa
    22381 ggaacttatt gctaaagatg ttaactttat atcaatagat acgttagaag aaccattaag
    22441 agttcaagcc aaaataagat attcagcaaa gccatcacca gctactattc atagagtagg
    22501 agaagatact ataaaaatag tttttgatga agctcaaaga gctattacta aaggtcaatc
    22561 tgtagtaatg tatgatggag atattgtagt aggtggaggt ataatcgaga aaagtttata
    22621 aaaacatcta aaaaattaaa aaaataaaca ataaagtata ttaagagtaa ataaagttta
    22681 taaaataaat ttattgtata tgaaaatata gtattgatta attttgtaaa atttagtaat
    22741 ataattagta tattattaaa aaacaaatac agtgataagg aatagtaaaa tatttgatgt
    22801 cttacagaga gagggaaatg ctggaagccc ttagaatatg atatttgaag cagcctttta
    22861 gtagcaattt tgaacaattt atcttttata aagaagattt aagtagagat tgacggtagt
    22921 tacgttatag ctaaggtttt ccgaagatat catactttgt atgataatta gggtggtacc
    22981 gcgattgtcc tcgcccctat aaatttattt atagggacga ggttttttat ttaaacgaaa
    23041 aaataaaatt tattaggagg aacatgatat ggaaaagatg ggattaaatg aaataagaag
    23101 taagttttta gaattttttg agtcaaaagg tcattatgta gcaaatagtt attctttagt
    23161 accaaataat gataagagtt tgttacttat aaactcaggt atggcacctt tgaaaaacta
    23221 cttttcaggt gtagaggtac ctccaagtgt gagaatgtgt acatctcaaa aatgtataag
    23281 aacaggagat atagaaaatg taggtataac tgcaagacat gctacattct ttgaaatgat
    23341 gggtaacttc tcatttggag attactttaa aagagagtca atcaaatggg gatgggaatt
    23401 tgttacagaa tggctaaata taccagaaga taagatatgg gtgactgtat atgaagaaga
    23461 tgatgattct tatgatatat gggcaaaaga aatgaatttc ccagaagaga gaatggttag
    23521 acttggaaaa gatgataatt tctgggagat aggtacaggt ccttgtggtc cttgctcaga
    23581 aatatatttt gatagaggag aagaatatgg atgtgacaat ccagactgta aaccaggctg
    23641 tgattgtgat aggtatttag aattttggaa tcatgtattt actcaatttg atagagatga
    23701 agaaggaaat tatagtctat tagaaaacaa gaatatagat acaggtatgg gtcttgaaag
    23761 aatgggatgt attatgcaag gcgtagacac tatctttgaa gttgatacaa ttaaatctat
    23821 actagaagca gttgagaagt taacaggagt aaaatatgga gagaatccaa aaaatgatat
    23881 atcaataaga ataataactg accacataag agctgtaaca ttcttagtta gtgatggagt
    23941 gcttccatca aatgaaggca gaggatatgt tcttagaaga cttcttagac gtgctgctcg
    24001 tcatggtaag ttattaggag taaaagaatt atttttacag aaactaatag atgaagttat
    24061 aaaagtaaat gataaagcat atccagtttt agttgaaaaa gaaagttata ttaaaaaagt
    24121 agttggaata gaagaagaaa aattcaatga aactatagac caaggaacag aaatattgaa
    24181 ctcttatata gaagtattaa aaaatgaagg aaagactgtc ctaagtggtc aagaagcttt
    24241 taaactatat gatacttatg gcttccctat agacttaact aaagaaatat tagaagaaga
    24301 gcacttatct gtagatgaag aagcttttaa tgaagaaatg gaaaagcaaa aagaaagagc
    24361 tagaaatgct agaggaaata tggatggaga aagttggaag gaagaccctc tatcaaaatt
    24421 agaatctaca gttgacagta catttaatgg gtatagtgaa atttatggtg aaggaactat
    24481 tgaagctata gttaaagatg atgagctagt tcaaagtgca aaagaagggg ataaggtatc
    24541 tatagtacta gataacacta ctttctatcc tgaaggtgga ggacaagttg gagactgtgg
    24601 attgataact aatgaaaact tagttttaga agtattaaat actaaaaaag gtgctaataa
    24661 tagtataaaa catataggta taataaaatc aggtagaata agtaatgggg acaaagtaaa
    24721 gacactcgta gatggagaaa ctagaatgtc agctgcaaga aatcacagtg ctacacattt
    24781 acttcataag gcattaagag aagtattagg agaacatgtt aatcaagcag gctctttagt
    24841 aactccagaa agacttagat ttgatattac tcactttgaa gcgatatcaa atgaagaatt
    24901 aaaagtaata gaagaaaaag taaataatgt tattttatca tctttagaca taaaatgtga
    24961 tattatgaat ataaaggaag ctaaagaaaa aggagctaca gcactatttg gtgaaaaata
    25021 tggtgatgag gtaagagtag tttctatggg agattattct acagaacttt gtggaggaac
    25081 tcacttgaca aatacttctc aagtaggaat gttcaaaata ttatctgaag gtggagtagc
    25141 agcaggtgta agaagaatag aagcaataac aggaaaagct gtttatgaat acctaaaaga
    25201 aagagatgga attatctctg aagtttgtgt aaacttaaaa tctaaagaag ataatttaat
    25261 tcaaagaata agttctttat tagaagaaaa taaaaactta tctaaagaat tgcatgatat
    25321 gaaagctaag atgagtttac aatcagttga ttctattttt gattctaaag ttgaagtaaa
    25381 tggagttaat ttgataacta ataaatttga aggtatggat atggatacac taagagaaac
    25441 agcagataat ctaagagata agcttggttc tggtgtagta gtacttgcaa atgtagtaga
    25501 tgataaagtt aactttgtag ttactgccac taaagatgta ttagataagg gaatacattc
    25561 aggtaatatt gttagagaag ttgcaaaaat agctggagga aaaggtggag gaagacctaa
    25621 tatggcacaa gcaggagcta gtgatgtatc taaggttgac caagctttaa gttatgcaag
    25681 tgaggttatt aaaacacaag ttaaataaca ttcactcaag gaggttttta gtttggaaaa
    25741 agatttagac tacactatga aatttgaagg aataccagaa gataggatga gtgtaggaga
    25801 tactattgat tttgtatata aagcattagt tgaaaaagga tataacccta taaatcaaat
    25861 tatagggtat ctcctttctg gtgattctag ctatataact agtcataaaa atgcaagagc
    25921 tataattaaa aaatttgaaa gagatgagat acttgaagaa gtaatcacgc actatctgaa
    25981 tagaaagtag gtcaaataaa tgagaaaaaa gattatgtcg ttgtttgcaa tagtcattat
    26041 gatttttgta tcaactgcta gctatggttt tgctgaagaa aaaggcaagg tcatatttat
    26101 tgatatgaat aggaccaatc ttggaagtat gatgaatatt ccaatcttga aaaatgagat
    26161 agaaaagaga ggctatgtag ctctcatgaa tataagaggg gatcaaggta ctgatgatag
    26221 aaggtcttat gcaagtatgg gtgcaggaac aagagctagt gtaactactg aagactatat
    26281 caattttcaa aaagtagacc aagatactgc taaagctttt gaagtcgcaa caggtcaaaa
    26341 agctaaagaa ataaatgatt taggcattaa tagatctata aatgaaaatc ttgagtatgg
    26401 agaatatgga gcaaatttag ggtcattagg aaaaacttta tcagaaaatg gtttgaagac
    26461 atctgtaatt ggaaatgctg atataattga aaataatcaa ttagttagaa atagaaactt
    26521 atgtttagta gctatggacc aatatggaag gattgattca ggtaatattg ataatattaa
    26581 taagaaagat tctaaaatgc catttggaat atctacagac tacgataaat tactagatga
    26641 aactaaaaaa tcctataaaa atagtgatgc tatatttgta gaacttggag atacatatag
    26701 attagatatg tatagattaa atctaaatga taatacttat ggtacaatga agaaaaatat
    26761 tgtaaaaaat ataaataaat atcttgaaca agtattttct atggttggag aaaatgacac
    26821 tgtttatata gcaagtgcat ttccaagtga cctagattat aagaataaga gaagactttc
    26881 tccaataata aaatttagtg gagaagggaa aggtctactg gaatctgcca caacaagaaa
    26941 ggctggaatt gttgccaatt tagatgttgg agtagatata ctaggtacat ttggtcttga
    27001 aaataaagag atggtaggta gaagttatac tttagtaaat aaagaggaca atattagcta
    27061 tctaaaatca ctttatgaaa ggatagtgtc tatatcttca ataaggtcaa ctattataaa
    27121 tggctttgta ggcgtaatat ctgcttcttg ggtaattgcc atgtttgcaa ttttctttag
    27181 aaattatatg ccacataaag agcgtgtatt caaagtactt aaggagttta taaaattagg
    27241 cataataatg ccactatcat ttatgttagc tcctttgatg aattttaaaa ctccaatttc
    27301 aatatctttg ggaatatttg caactacgct aatattatac ttgttaggta gaaaattatt
    27361 taaggacaat gatataaagc acatgggatt ttttgcaaca cttactattt tagtcatagt
    27421 tatagattct gtatttggaa catacttaat gcaaaataac ataatgagtt atgatgctgt
    27481 cataggagct agatactatg gtgtaggaaa tgaatatgag ggtgtaacta tagcaagtgc
    27541 agtatttggc ttagcagtat tattacatta taagaaagta tctaaattac ttgttgtaat
    27601 tttttcgtta ataatactta taactagtgc atatccttca atgggtgcaa atgttggtgg
    27661 agctatatca gaatgtgtag catatctgtt gtttataatg cttatatatg acattaaaat
    27721 agattttaag aaagctgtat tattagctgt ttcagctgta gtcgtcgttt ttgcatttgc
    27781 agctttagat atactttcag gtagtgaatc tcatttaggt atgtttgtac agagaatttt
    27841 cctaaatgga ccaggagaga tattacaaac atttggaaga aaaattcaaa tgaatatgca
    27901 actagcacag acaagtgtat gggtaaatat tttacttgta ggaattggag taattgcagt
    27961 acttatattt aaaccatcta gacactttag aaagataatg aataattttc caatcttgtt
    28021 taaagggttt attgcatcta tggttggttg tataattact cttttagtta atgattctgg
    28081 aatagttgct gcatctacag catctatata tatacttata ccactaataa taataagtat
    28141 aaatatgata atatttgaaa aaaataacta aaaaaatttt tactgttcat ataggattta
    28201 aataaaaaat gcaatatgat tattaatgta aactggatgc ttacataaaa aatcatattg
    28261 caatttttat gattgattat gcaaaatttt atgaagcagg aagcttgtga ccttagttat
    28321 gagaggttca gaatatgtat ttaatctata gaatataaat agataaagga ggaactatgt
    28381 tagatggaag aataatgggt cttgatgttg gtgataaaac tattggagtt gctgtgagtg
    28441 atttgatggg attaactgca caaggtgtaa aaacaataaa aagagttggc aagaaaaaag
    28501 atattgaaga gctaaaagca ataataaaag aaaagcaagt aaataaaatt gtatcaggat
    28561 taccaaaaaa tatgaatgga actttaggtc cacaaggaga aaaagtaatc aagttttgtg
    28621 aattggtaaa agcagaaaca ggtattgatg tagagttttg ggatgagagg ctttccacag
    28681 tagcagcaga gaggtcttta ttagaggcag atgttagtag acaaaaaaga aaaaaggtta
    28741 tagatatgtt ggcagcagta ataattttgc aaggatattt agattttaaa ataaattcat
    28801 aaaagaacta taaaattgaa tacttatgat atataatata aataataaaa ataaaaataa
    28861 atttgaggtg aatgacatta tggaagaaaa tataataaat ttaatagacg aaaatggcgt
    28921 tgaaagccaa tttgaaataa tattaacatt agaagcagaa ggaaaagagt atgcaatatt
    28981 aatgccttta gatgatgaag aagctgaaga agctttaatc tttagaattg atgaggatga
    29041 agaaggtgaa atcttaatac ctttagaaag tgatgaagag tatgaaactg ttgtagctgt
    29101 gtatactgct ataatggaag aagaaggttt aaattacgac gaagatgaat caaatggact
    29161 taactagata agtaattaag tttgtatata aaaatcaggt atttattata tttgctttaa
    29221 ttaaacctta ttaattgtaa aagcatttat agtatatatc tgtttttttg ttttttatta
    29281 aaaatagcaa aaaatttatg aattttggtg aataatatat cttttatggg tatatattta
    29341 tattgtgaga ataaattaaa tttatttaaa tgtcttgaca aaaaatgaat tatcaattac
    29401 aatattattt aataaagatt atcaattgaa aatagtaagg gtgatgttat ggcaaataca
    29461 atggatttat taaaagacaa gttaaaagaa actggtttta agattacgcc acaaagaaga
    29521 gcaatagttg aaatattgct taaacatgac cattcacatc taagtagtga ggaaatctat
    29581 gatttagtta gggttgactg ccctgaaata ggcttagcta ctgtatatag aacaatgcag
    29641 ctattggatg aaataggatt aatatcaaaa ctaaacttag acgatggatg tataagatat
    29701 gagattagtc tacataaaga agactgtcat aatcatcatc atcttatttg taaaaattgt
    29761 ggaaagataa tggaagctaa agaagacctt ttagacaata tagaaaaaga gatacaatca
    29821 ttgtacaaat ttaaaatact tgatcatgat gttaaatttt atggattatg tgacgagtgt
    29881 aatggagtaa gcgattcaga agaataaact tctattgctt aatgtcgatg gaagaatagt
    29941 atagaacttt tatattctat gctattcttc cattttttat tataaaccat ctataaaatg
    30001 caaatataca gttttcaaag tgattatagt tttaatattg aaaatataat taatcctata
    30061 agtacaaaaa tgaatgattt ttctatgaaa ttactaaatg gtcctccaac agttaaaaac
    30121 cctaaagaaa ttttcttttc gcttagcgga taaaaaattg gtattccttg ctttgtaaat
    30181 aaatctccta aaaacatatg gcttgcataa ccaatcgtac cataaaaaga taaattatta
    30241 atattagtga ttaattctat ctgttttagt aaaaaatata ctattatagt tgcaaatatg
    30301 ctatgagaaa aacttctgtg ctttaaccag ggaaaaattg acaacattag acaaaaaatt
    30361 gtaaaatata tttcaacttc tatgatgtat aagcatatag ctaatagtat aaatattaat
    30421 gatagaaaaa cttttcttag aaatgtgtgg gttagttttg cttcaataat tataattgca
    30481 acaaatgtaa ttattgcgct taaataaaaa ttatcattta tatttattga aataaagaaa
    30541 ataataatat taacagcata tataaaaatt ctatatatgt atttggatac attgtttttt
    30601 agaatgaaat ttgagaccat agaattagat ttatccaaat cgggtagtac agcaaaagta
    30661 gctgaaacta aaatatcaac taaagatata ggaattttaa atattaaaga tgtctggata
    30721 gctgttaaga ttcctattgc acaatgagtt ttaccacgca taaatcctcc taaattatcc
    30781 gatattaaat catataatat ttagaggata tcaaaataaa ataggtttgt caaaataata
    30841 cgttaaatgt attttaaata tagcttaaaa tatgatacga attatggtac aataagaata
    30901 actggtagcg agtgcgtgat ttgatattat ttatgattgt agaacgtgtt aaaaaattaa
    30961 atagacagaa ggagatgatg caattgttca aaaagaacac caacaagata aaggtaatgg
    31021 ccttgggtgg acttaacgaa gttggaaaaa atatgactgt tgttgagtat aaagatgaaa
    31081 taattgttat agatgctgga cttagttttc cagaggatga aatgttagga gtagatatag
    31141 ttataccaga tataacttat ttagtaaaaa atagggataa aataaagggt atatttatta
    31201 cacatggaca tgaagaccat ataggagctc ttccatatat attaaagaaa ataaatgtac
    31261 ctgtatatgg ggcaagactt agtataggtc ttatacaagt aaaacttaaa gaacataaga
    31321 tgaataatgt taagttaaat gttataggtc ctagacaagt tataaagctt gataatatgg
    31381 aagtagagtt tttgaaaaat aaccatagta taccagatgc ttactctatt gcaatacaca
    31441 cagaccaagg tataatatac cacacaggag attttaagat tgacttgact cctattgatg
    31501 gagatgttat ggatatgcat agaatctgtg agttgagcaa aaaaggagtt cttttaatgc
    31561 tggctgatag tactaatgca gaaaaaccag gatttactat gtctgaaaag actgttgggg
    31621 taggtcttga cgaattattt gcaaaaggaa atggcagaag aattatagtt gctacctttg
    31681 catccaacat acacagatta caacaaataa taaatactgc tgagaagttt aatagaaaag
    31741 ttgctatatc aggtcgctca atggtcaatg tagttggagt tgcaaaagaa ttaggatatc
    31801 tagatatttc tgatgacatg cttatagatt taaatgatat atgtaagtat gaagatagtg
    31861 aattagtgat aataacaact ggttcacaag gtgaacctat gtcagctctt gctagaatgg
    31921 ctttttcaga gcacaaaaaa gtagaaataa agagtggcga tttagttata atatctgctc
    31981 atccaatacc aggaaatgaa aaattaatat caagagtaat caatttctta tttgaaaaag
    32041 gtgcagaagt agtatacagt gatatagctg atatacacgt ttctggacat gcctgtcaag
    32101 aagaattaaa acttatacat gcattagtta gaccaaagtt ttttatgcca gctcatggtg
    32161 aatatagaat gttaaaaaga catgctgaaa tagctgaaca gcttggtatg gataaagaaa
    32221 atatttttgt catgcaaaca ggagatgttt tagaattaga taaaaactct gctaaggtag
    32281 caaatcgtat acaaacagga aacatattag ttgatggtct aggagtaggc gatgtaggta
    32341 atatagtttt aagagataga aagcatttat cagaagatgg tcttatgata gttgtagtta
    32401 ctatttctaa ggatgagggt aaagttttag caggtccaga tataatttcc agaggattcg
    32461 tatatgtaag agaatcagaa gatttaatgg atggagcaaa ggatataatt aagaatgttc
    32521 taaatgaatg tgaagagaaa aatattaaag aatgggctta tttaaagaat aatattaaag
    32581 aaaacctaaa agaatattta tatcaaaaaa ctaaacgaaa tccaatgata cttcctatca
    32641 taatggaagt ataatataaa aaaactataa atttgaaaat tagacctctt gtaattttta
    32701 aagtaatatc aaaaatccaa ttgatattat cttaagaatt acaagaggtc tttttaatac
    32761 ttgatttaaa aaaatatata tattacataa aataccaatt ataacaatct tttattagta
    32821 aaaactactt atatataaat tttactgata ggaggattct tatggatgat gtttcaagac
    32881 aaaatgctat aaaagcttta aaacaaacaa aaatggaaat agcaggagag tatggaatga
    32941 attatgaaga tgcttttgaa ataatagaaa atgcgtctaa taaaggtgtt ttagaaggtt
    33001 actttaaaaa gcttgaaaag aaaaaaaatt taggtcaagg tatatctagg catttagaat
    33061 aaaaaaagtt aaatgtgaga aaataagttt aaataaactt taagaggtga catcgatgaa
    33121 aaaattagct agtactgctt tggctatatt aattgcgctt actcctttaa gcttttcttt
    33181 tgcaaataat aaggaaaatg ctgatgccaa ccaactaaat atatcttcaa aatcagcaat
    33241 tttgatggat gttggtagtg gacaaatttt atatgaaaag gatgctcata aaaagcttcc
    33301 tcctgctagt gtaactaagg taatgactat gttacttata gtagaagctt tagattcagg
    33361 caaaattaaa cttgatgatg aagttcaagt aagtgaaact gcatctagta tgggtggtag
    33421 tcaaatattc ctagagccag gagaaactca aaaggttgat acattattaa aaggtatagc
    33481 tgttgcttct gcaaatgatg cttgtgttgc tatggctgaa cacttagctg gaagtgttga
    33541 agggtttgtt gatagaatga atgctaaagc aaaagagtta aatatgaatg atactcattt
    33601 tgcaaataca aatggacttc cagttgcaaa ccactataca tctgctcatg atatagcttt
    33661 aatgtctaga gaactattaa aacacgaaat gataagtaaa tacttaacta catggatgga
    33721 taaggttgta gtagggaaga aacaagttac agtaggtctt gcaaatacta ataaacttat
    33781 aaagcattat caaggagcaa ctggtgttaa aactggcttt actcaagaag caaaatactg
    33841 tttatcagca tctgcaaaga gaggtaatac tcatctagtt gctgtaactt taggagcaga
    33901 gacttctcca gaaagattta acgacgcatc tagcctttta aactatggat ttgctaatta
    33961 tgaaagtgtt aagttatgta gtaaaggtga taatatagca acacttacat tggataaggc
    34021 tgatgaaaac aaagttaaat tggttgctaa agaagattta aatgctctta ttaaaaaagg
    34081 aagctctaaa gaatttgaga agaaaattga aatagtaaaa aatcctaaga tgccaattaa
    34141 aaaaggaact gttttaggaa agataaagat atgtaaagat aaaaaagtaa taggcgaagt
    34201 ggaattaata aatactaaag atataaataa ggcaagctat ctacaaatgc ttcaaagaat
    34261 aattgataac atgatataaa taattatcaa atgagatata gtacacagag atataattat
    34321 aatacacata aaaaataccc ttatgtaatt atttgccata ggggtatttt tattatcata
    34381 taaaagtatt tcataatttt tatagacaaa tataaaagtg tatgtaactt ataatatcat
    34441 ttggtttaat atcatgtaca tgattattta atacttatcg aagtaaaggc atactaccag
    34501 taagtttatt cactttcttt ttattaccat aaattgctat cccaaaatac tgtaactcat
    34561 cttctggaac actagcaatc ttttgtgtaa attcttcata aagattgcaa ctttgtgcta
    34621 catctgaaaa atctacaaca gttaaatcgt taaattccaa agtatataat tttttacgta
    34681 aatcttttat tatttctttg tttcctttta aaataggtac aggaatgtca ataattccta
    34741 agtggctatt acctgtttta tcaattacgt ctggtccaac agtttctggg gcatgttttc
    34801 ctagtgtaat tcccattata gctgctgtat tggatataat tcccattggt aaattttcgt
    34861 ctattaccat gacacatttt gaatttttag tttccatttt tatttctcct ttttacctta
    34921 tttaaagttg tttgaatcat catttataaa tatattttga tactgctttg gtgtaagacc
    34981 aataaaattc ttaaaaaagt ttgtaaagtg actttggtct acaaaccctg tttgtatcgc
    35041 agcatctata ggttctacgc ctttttctaa aagcttttta gctttgttta cccgtatatt
    35101 ttctaggtat ctatatggtg taataccttt taatttcgta aaattacgaa gtagagagta
    35161 tttattcata cctgctatag tgctcaattc atcaagtact atatgttcag cataattatt
    35221 ttctagataa tcacatactg cttgaatttc tatatttgta ttttccaagt tagattgcaa
    35281 atttggctct gtatgttctt caattaattg ttcaattagg aagaaaaaaa gctcttcctt
    35341 tttaaaatcc aattcttctt ccatgattat atagtgtaat tcttgtagta gaggtactag
    35401 ttcactgcga aatataacag gttgattaaa ttttggtaaa taatttttac ctgtaatttc
    35461 aaagacagtt tttttcatta tttctggctt aatattgata catctgtaat caagtacttt
    35521 atcatctatt tgttcacaag tatgactatc aaaagggtta aaaagtagca aatctccagt
    35581 acttgtggta tactctttat ttttacaaga taaatatctt tgaccttttt ctataaaacc
    35641 aattacataa tgttcatgaa aatggtttgg gaatttttgc attatgcctt taaatctata
    35701 tgcttcaatt tttaactctc tatcaaagca tactgtgcgt tcctcttgtt tcacttaggt
    35761 aacctccttt catgttatac aaagtgtaac atatattttt ttacacttct tgtatgatat
    35821 tgactaaaac ataataatgt caattcatgt caatataata ttacacttac agtagtgtaa
    35881 ttattatatt ttcacaacga atatgatata ataataaaag ttataaaata aaaagcaaat
    35941 tttaatttta tgcctagaag ttaaaattat tataatataa aataattttg attataagtt
    36001 atttaattgt tataatagta ttaaaaattt aatttaggag atgtaaatta atgttaagta
    36061 tgaatatagg agaaatagta gcaagtttag ttggtatagc tatggcaata tcaatacatg
    36121 agtttggcca tgcttattca gctcacttat tgggagatga tacagctaaa gcttatggta
    36181 gaatgacatt aaatcctgta agacatgttg acccaatcgg tcttttggct atgtttattc
    36241 ttagaatagg gtgggcaaag ccagtaccag taaatccaaa taactttaaa aactataaga
    36301 taggaaactt gatagtttca cttgcaggcg ttttttgtaa tgttttgacc gccatacttt
    36361 gtgtaattat aaataagtat gtgcatatgt atgctataaa tacaatagct gaatatgtgt
    36421 tcttatatag tattggattt gcagctttta acttacttcc tatacctcca cttgatggat
    36481 ggggagttat atctacattt gtaccatata aatggaatga atatgtgtat aaatttgaaa
    36541 gtatgagtta tataatttta ttaattgctt tatttactgg tatatataga ataatattaa
    36601 caccaatata tgcagtatta ctaaaaatag tatatttatt tgtttaatat aataaacaga
    36661 ttaaagaggg attgatatga agtataatat acaattacaa gtctatgagg gaccattaga
    36721 tttactttat gatttaataa caaagcataa aatagacata aaggatatat ctataataga
    36781 tataactaag cagtatctaa attatttgaa aatgctagat aaaatggatt tagagataac
    36841 tagtgaattt ataactatgg catcaaaact tttagagata aagtctaaat atctacttta
    36901 taagcaaaaa gatgaagaag aagacccaag aatagaactt atggaaaagc tagaagaata
    36961 tagaaagttt aaggtagcat cacaagatat aaaagaaaat ataacttatg ttaatgagag
    37021 attttataga aataaagaag agataattat tgatgacaat gtagatttag aagatatatc
    37081 aatagaatca ataaaaaata tacttccata catatttaaa gttaaaacat cacagataga
    37141 aaatgctaat gatgagaaac tagataaaat agtaaggaag aagataatat ctgtagaaga
    37201 aaagatattg tatataagag atattataaa agataagatt gaagttactt ttacaaatat
    37261 tataaaatca tatgaaaatg atgaaataat agctactttt ttatctatat tagagcttat
    37321 aaaagaaaaa gaaatagtag tagtacaaga tatatttttt gatgatattc taataaaaaa
    37381 aagttcggag tgttaaaatg aaaagagaag atataaagta tattatagag tctgttatgt
    37441 ttgcatatgg ggagccgata agtataaaag agttaaacta tataataaat aaagaacttt
    37501 catcaaaaga aatagagatt atgttaaatt tacttattga ggagtacaga gaacaaaata
    37561 gaggtattca aataatcaag ttagagaata agtaccaaat gtgtacaaat aaagactatg
    37621 cagaatatat aaagaagatt attgaaccaa agaaaaagaa aagtttgagc caagccacat
    37681 tagaaactct tactataatt gcctataaac aaccaataac aaaggtagaa atagaggata
    37741 taagaggagt aaaatgtgat aaagtattac aaactttgtt tgaaaatgaa cttataagag
    37801 aagctggtag attgaataag atagggaaac caataattta taaaacaaca gatgaatttt
    37861 taaagttgtt gaatatagaa agtcttgaag aattgccacc tatagaaaat tatcaagaag
    37921 tggcaacaaa tgaataaaaa tataaaatca caataattta gtatgtaatt ttaaatttta
    37981 aaagatttaa aatgctctaa gttgttgtgt ttttttgt
//
LOCUS       DAEPXF010000015        37076 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain HB27
            SAMN13376389-rid12479423.denovo.016, whole genome shotgun sequence.
ACCESSION   DAEPXF010000015 DAEPXF010000000
VERSION     DAEPXF010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13376389
            Sequence Read Archive: SRR10518432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 37076)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37076)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001069624.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001069624.1
            Genome Coverage       :: 315x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/15/2021 01:54:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,165
            CDSs (total)                      :: 4,076
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 89
            rRNAs                             :: 10, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 10 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 19 of 69
            Pseudo Genes (incomplete)         :: 44 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 10 of 69
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37076
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13376389-rid12479423.denovo.016"
                     /strain="HB27"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="China: Hebei"
                     /collection_date="2017"
     gene            <1..522
                     /locus_tag="KRL67_000709"
     CDS             <1..522
                     /locus_tag="KRL67_000709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2953 domain-containing protein"
                     /protein_id="HBH1521614.1"
                     /translation="ADINNKDICLKLNIKYMFNLININRQLYPAENSKNNDKKEGMKN
                     NIDSSILLADDLLSIYRLLKKIKIHELYSNINFGTENIGLTSSVYVLINTLYGNLFNI
                     IDAEKMYLNVNPDFTKDFVLGNIRIHIRPRIKALFNIIIMINKIMNKNKGNKEGDSNE
                     SNRFDTESYGNNS"
     gene            476..904
                     /gene="ytfJ"
                     /locus_tag="KRL67_000710"
     CDS             476..904
                     /gene="ytfJ"
                     /locus_tag="KRL67_000710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GerW family sporulation protein"
                     /protein_id="HBH1521615.1"
                     /translation="MRATGSIQSLMETTLETIKGSIDANTIIGDPIKTDTTVVVPISK
                     VTIGFGIGGGEYSKGYDDKDREIELKNEKSDTNFAGGSAGAISVQPVAFVVVESGETR
                     IMSLDSNINLVDNILSITPRVLEKIQNISQSNKNQDKNNM"
     gene            1152..1592
                     /locus_tag="KRL67_000711"
     CDS             1152..1592
                     /locus_tag="KRL67_000711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HBH1521616.1"
                     /translation="MSDKELSILLLESMNNFHNLIKIINNERYKKDKVLTERQFFALV
                     KIRKHDKIELKNLSRDLHVSTSSLCILLNKLVEQEYVYREEDSRDRRNTFYGITKNGE
                     KILDNEILKFVSIISDKMDCLDIDNKDKLFTSLEESKNIIEQLF"
     gene            1608..3059
                     /locus_tag="KRL67_000712"
     CDS             1608..3059
                     /locus_tag="KRL67_000712"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HBH1521617.1"
                     /translation="MNIKYSKEILKSLENAITVVALFEGEDVSTTFDKINMISRNFNS
                     KENSIDTVTLVEHDNLVNVCLVGFGKRELLNREKIRIIGGNLCKKLKNILKKEAYKSS
                     DVNILNLNLNSDEIGSFVEGILLGNYKFDKYKTKSKDFERKEVDTMTIFTEKEVECQI
                     EKAKILANSTIIARNLVNEPSNIIYPKTLAIETVKLGAEFGFNVDVYEEEKIKALGMD
                     AFFAVSRGSINKPRFIVMRYFGDKESTDILGLVGKGLTYDTGGYSLKSNASMLDMKTD
                     MAGAASVIGAMCAISQCKLKKNVIAVVAACENALSGGSYKPGDIISSMAKKTIEVLNT
                     DAEGRLTLADAIYYIINNEKVTKVVDVATLTGAALTLLGNVATPIVTNDDDFYCELEK
                     AATLSGERVWKMPIYDEFRDMIKGEEADLKNTGGKNAGCITAGAFIGEFVGSTPWIHM
                     DIAGTSTSSKSIGYKAKGATGEPVRTLYYLAEI"
     gene            complement(3150..3818)
                     /locus_tag="KRL67_000713"
     CDS             complement(3150..3818)
                     /locus_tag="KRL67_000713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YwaF family protein"
                     /protein_id="HBH1521618.1"
                     /translation="MFKIFLFSSEQFVSLFIFGLFLYYCPKLTKNILPYSYTVEKIIC
                     TLLVIIMALEQLLLISSGNYSTLNSLPIGINYICIYLCIAILIFKQYHLFNIFFSWSL
                     VCSVGELIFSKNLGYEFPSLIYFIFILSKCLIIYAEIYMVDVRKFKVNRYALRDNLAI
                     CFIYFSFIFLLNTFTNSQYYYGFLSHSTTAIFTFIFVTSIMYIPTLLFNRDTFILEKK
                     KKSK"
     gene            4087..4227
                     /locus_tag="KRL67_000714"
     CDS             4087..4227
                     /locus_tag="KRL67_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH1521619.1"
                     /translation="MGIIESASKLAEMVHLLAVEKGITDIEAWDEAVKEYSKIYEERR
                     NE"
     gene            complement(4402..4722)
                     /locus_tag="KRL67_000715"
     CDS             complement(4402..4722)
                     /locus_tag="KRL67_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG-like family protein"
                     /protein_id="HBH1521620.1"
                     /translation="MKLKTISLPELNNLDPTLESTFIKMGEEQGELAECIGKFRNLSG
                     ENNDLDEVDIIKKTAKELMDVAQTCVTMMFKLEEQYGINLDEIRKEHIKKLEKRGYIK
                     NMDK"
     gene            complement(4842..5579)
                     /locus_tag="KRL67_000716"
     CDS             complement(4842..5579)
                     /locus_tag="KRL67_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HBH1521621.1"
                     /translation="MDANSLKDLIANHNNIVFFGGAGVSTESNIPDFRSSTGLFSQKL
                     NKQFTAEQLVSHTFFVRYPEEFFEFYKDKLIYPNAKPNNAHIALAKLEEMGKLKAVIT
                     QNIDGLHQMAGSKNVLELHGSVHRNYCTKCGKFFDLESMLNLGGNIPYCDNCGSIVKP
                     DVVLYEEALDSDVITKTISAISNADLLIIGGTSLAVYPAASFIDYYKGDYIALINKAN
                     TVYDKSASLVINKPIGEVLYEAVLRQI"
     gene            5794..7617
                     /gene="acd"
                     /locus_tag="KRL67_000717"
     CDS             5794..7617
                     /gene="acd"
                     /locus_tag="KRL67_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosaminidase"
                     /protein_id="HBH1521622.1"
                     /translation="MKKKAALATLAMLPLSVVNAHADGDIGIVTINYLNVRNEPTAES
                     SIAFVAKKDDKVLIKDSSNGWYKIKAESGQEGWASSKYIAKSNSDSLRTSTNKEKQVT
                     SNSLNMRNGAGTSYRVITVLKKGQKVEVISESNGWSKIKYDGRLGYVSSSYLGDVSNS
                     TNKSKTKQVNTTSLNVRSGPNTSYGLLGKLPKGSKVEVISESNGWSKIKYNGKDAYVS
                     SMYLSDVSQSNSDNSSQSNDKKNTDKVVNTASLNVRSGPGSTYSKLGKVYKGSKVTVL
                     SESSGWAKISFNNKEAFVVGNYLSTSADTSNNNSNSNSDNSSNNNGNNSSSSGQVNGM
                     SGISGAKIDYKSLSYTLESHISKQVEKAVSGGNVIAPSNRKSTPSPEFSTFSAQRTSS
                     FVNASSSDIEYYLNPKNFTNTTKGMMQFLKINSYRGGISESSLNSYLNGLSSSVFKNQ
                     GAAFINAAKKYNIDVVYLVSHAMWETAYGKSTLAQGQTLTSYKGQPLSKPVKVYNFFG
                     IGAIDKSANVSGAEAAYSNGWTSVEATIDGSAKWISQNYVNSSKYNQNTIYKMKWNYD
                     YTWHQYATDVNWANGISGIMENLIGLYGGGSSLVFEVPQYK"
     gene            7832..12130
                     /locus_tag="KRL67_000718"
     CDS             7832..12130
                     /locus_tag="KRL67_000718"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PolC-type DNA polymerase III"
                     /protein_id="HBH1521623.1"
                     /translation="MESIKEYLDKLEINNSGLGKQLKEVYINRVVYFKEDKIVYFYLT
                     SKDIVSHELLDKFKEELMYKLDYFKDMRMKIRFTGLERKSNKDVIKKYWNNILYILKY
                     LCPSIAGWYKQVEFLCLEEELKIKLPKGIFYERLMKKNVTYVLKTVLSEELGLDLNIT
                     IEKAVDEKVNKERLIRINDREMEEKIRALEIGKVNNCEENEEESYVIKSDVDENLIYG
                     DNANAMIENIVELNASSGTVAVVGDIFDVDTKELKNGKILMIASITDYTSSISCKLFL
                     TDTNKDGVLESVKKGAYLKIKGDIVYDTYQREISMMISGIRKETRIEREDKSEEKRVE
                     LHAHTQMSSMDAICSVKKLVERAAKWGHPAIAITDHGVVQGFPDAMNAGKSNNIKILY
                     GVEGYLVEDDSLIIEDANDKELSQTFVVFDIETTGFSNTNDKITEIGAVKIENFKIVD
                     RFSELINPEKDISYKIQELTGITNELIKDKPTIEEVLPKFMEFVGDSVLVAHNAEFDT
                     GFISQKCREQGLLYNNKKVDTLMLARVMLPNLKRHRLNVVAKELGIPLLNHHRAVDDA
                     EATALIFNKFLQMLTEKGAKTLSDVNNILGKIDYTKLSTNHITLIAKNSIGIKNLYKI
                     ISDAHVNHFFRAPRILRSVLNEYKEGLIIGSACEAGVVFQAVKKNVSDEEMKKIIDLY
                     DYIEVMPIDNNRFMIDKGEVKDEEELRELNRKLIDTAKKFDKIPVATGDVHFLDKHEA
                     ILRKVLKYSQGFKVDEEETYLHFRTTDEMLEEFSYLGEELAYEVVVENSNLIADMVED
                     IKPIPDDTYPPIIEGSDVELREMCYKKAKRIYGDPIPEIVQKRLDRELNSIISNGYAV
                     MYIIAQKLVAKSLSDGYLVGSRGSVGSSFAATMSDITEVNPLPAHYICENEDCKYSFF
                     YEIGEWGSGVDLPDKDCPKCGRKLAKNGHDIPFEVFLGFEGDKEPDIDLNFSGNYQPT
                     IHKYTEELFGEGYVYRAGTIGTVAEKTAFGYARKYVEENNISVPNAEVLRLSNGCTGV
                     KRTSGQHPGGVMVIPDYKDVYDFTPIQYPANDTSCGVITTHFDYHSISGRILKLDILG
                     HDGPTIIRMLEDMTGINITEIPLDDKETMSLFTSTEALGVTPEEINCPIGCLAVPEFG
                     TKFVRQMLLDTKPTTFAELVRISGLSHGTDVWVNNAQDLVREDIVGLKDVISTRDDIM
                     NYLIFKGLPPKMSFTIMESVRKGKGLKPEHIEEMKKNNVPEWYIGSCKKIKYMFPKAH
                     AVAYVMTSFRIAYCKVHYPEAFYATYFTTKVEDFDADLIVKGLDAIKSKINEIESLGN
                     DATTKEKGMLTVLEVALEMYARGIKLLPVDIYKSDATEFIVVGEKTLLPPMAAIQGLG
                     GNAAINIQNERKNGEFISKEDLRKRTKISKTVIETLTNHGSLENMSERNQLSLF"
     gene            12334..12795
                     /locus_tag="KRL67_000719"
     CDS             12334..12795
                     /locus_tag="KRL67_000719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HBH1521624.1"
                     /translation="MKKNLEATIEEIVTKITDEHGFEMVDVEYVKEAGEYYLRVYIDK
                     EEGISLNECELVSRELSPILDEKDPIKENYFLEVSSPGLDRALKKDRDFVRYQGRDVD
                     LKLYKPLNGCKQFEGELVGLTEDNNIKIIVNGKEMEFNRKDVAIVRLAIKF"
     gene            12825..13979
                     /gene="nusA"
                     /locus_tag="KRL67_000720"
     CDS             12825..13979
                     /gene="nusA"
                     /locus_tag="KRL67_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HBH1521625.1"
                     /translation="MNHEFMEALDELVVDRGIDKEILIDTIEQALLTAYKKNFGSAQN
                     VRVEFDRDKGDIKVFSQRVVVDESDLYDTFLEIELADAREINPNYELGDIIEHEVTPK
                     NFGRIAAQTAKQVVVQRIREAEREIVYNEFIEKENEIVTGEVARANKNVVYVNLGRIE
                     GIMTQSEQIPFENYKAGQKIKFYILEVKKTNKGPQIVVSRSHPGLVKRLFEEEVPEIY
                     EGIVQIKSVAREAGSRTKMAVRSIDEKIDPIGACVGPKGLRVKNIVDELGDEKIDIIK
                     YSEDPAEFISAALSPSKVVRVDIDEDEKSALVIVPDYQLSLAIGKEGQNARLAAKLTN
                     WKIDIKSESQAEQEMLNLENSKEIEAEDVEAEFIDQEELDTSEDNFSEEE"
     gene            13986..14267
                     /locus_tag="KRL67_000721"
     CDS             13986..14267
                     /locus_tag="KRL67_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HBH1521626.1"
                     /translation="MILKKVKKVPQRKCIACQDRDSKKELIRIVKNKEGKIFLDPTGR
                     ANGRGAYVCKSSECLKKAIKSKALNKAFKIDVPDEIYDNLLMELEEYEK"
     gene            14257..14568
                     /locus_tag="KRL67_000722"
     CDS             14257..14568
                     /locus_tag="KRL67_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal L7Ae/L30e/S12e/Gadd45 family protein"
                     /protein_id="HBH1521627.1"
                     /translation="MKNNKEKVLSFLGLATRAGKIVSGDDSTLLDLKKGKVNLILIAE
                     DASNNTKKLFKDKSTFRNIPYLFFSTKEEIGFAIGKSPRAVVGIKDENFSKKIIELIE
                     I"
     gene            14590..16530
                     /gene="infB"
                     /locus_tag="KRL67_000723"
     CDS             14590..16530
                     /gene="infB"
                     /locus_tag="KRL67_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HBH1521628.1"
                     /translation="MSKTRVYQIAEELNISNEELINKLAELDINVTDKDSVLEGEELE
                     LALEMLGEDLSQENGNVIEIDGKLTVQVLATKLDKSPSEIIMKLMKMGTMATINQEIS
                     FEIAALAAKDYGFELTVAESDDTEALEIEALMEIEEDKEEDLKPRPPVVTVMGHVDHG
                     KTSLLDAIRKTDVISGEAGGITQHIGASEVKINGHKIVFLDTPGHEAFTSMRARGAQV
                     TDIAILVVAADDGIMPQTVEAINHAKAAGVPLIVAINKIDKPGANPDKVKQELADQGL
                     LVEDWGGEVIAVPVSAKKKEGIDTLLEMVLLVAEMEELRANPNKRAVGTVIEAELDKG
                     RGPVATVLVQGGTLTVGDPIVAGVACGKVRAMINAKGKRVKTAGPSTAVEILGLSEVP
                     QGGDQFVEVPTDKIARSVAARRQQIVRDEMLKSTQRLSLDALFSQMSEGSIKDLNIVI
                     KADVQGSVQAVKQSLEKLSNEEVQVKVIHGGVGAVTESDILLAAASNAIIIGFNVRPV
                     PGAESLGEKENVDIRTYTIIYKAIEDIQAAMTGMLDPEYVDEETGKAEIREIYKISGV
                     GTVAGCYVTNGKIFRNCKIRLVRDSIIIHEGELAALKRFKDDVKEVNSGYECGMSFVN
                     YNDIKEGDIVEAYITKEVERKL"
     gene            16573..16953
                     /gene="rbfA"
                     /locus_tag="KRL67_000724"
     CDS             16573..16953
                     /gene="rbfA"
                     /locus_tag="KRL67_000724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HBH1521629.1"
                     /translation="MASYNRTRRIAEEIRKVVSTMLINGVKDPRITSMVSVTDVEVTN
                     DLRYAYVYVSILGGDEESTLTGLKSAGGYIRREVGKNIKLRYIPEIVFKLDDSIEKGM
                     YMDSLIKRVNEKNAQQNEDENYDE"
     gene            16934..17917
                     /locus_tag="KRL67_000725"
     CDS             16934..17917
                     /locus_tag="KRL67_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HBH1521630.1"
                     /translation="MKIMMNNMDSIINYIYEGDYFIVTSHISPDGDNIGSTLSMYYTL
                     KNLGKNVYYVLDDEAPLNLRFLVEGIKIYKSNEFNMKNYSLIALDCGDKMRICVSDEI
                     KNNASKLICIDHHASNDSYGDLNYVDIDASSTCELVYNLLVRFQQTKDINIINEDIAT
                     CLYTGLVTDTGNFSYSNVHASSFEMAKNLLVLGAQKNTIIQNIYQSNSSDYYKLLGEA
                     LKGLEIFNSKISSIVLTQDMMNRNNISFNDVDGITTYTRDIKGIEVGILFKEKKQNEI
                     KVSFRSKNYVDVSEIAKLFGGGGHVRAAGCTIRDSIDNAKKMVLEAVLKSI"
     gene            17930..18835
                     /gene="truB"
                     /locus_tag="KRL67_000726"
     CDS             17930..18835
                     /gene="truB"
                     /locus_tag="KRL67_000726"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HBH1521631.1"
                     /translation="MNKIISILKPTGMTSHDVVSRVRKILNIKKVGHTGTLDPDASGV
                     LPICIGKATKVCEVILNKDKSYICELTLGISTDTYDASGEILKKVDDFKFSNEDIERA
                     FDTQRGEINQLPPIYSALKVNGKRMCDLVRSGRQSEITLKTRRVNIKDIKILSIKGNK
                     VMFYVECSKGTYVRSICHDIGEYLGCGAHMSFLNRTSSGKFDLDNSITLEELELFYEN
                     KTLDKYLYDIDYVLDSFNYVVLNPNAIKYYSNGGSIDDKRFLKNNFDKDDEFVRVYST
                     DNFLGLGKLSKHNNTISVKSDKMFI"
     gene            18966..19895
                     /locus_tag="KRL67_000727"
     CDS             18966..19895
                     /locus_tag="KRL67_000727"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HBH1521632.1"
                     /translation="MVIIKSIKEIANIKESVITIGNFDGVHKGHQVLIGKTVEHAKKE
                     NIQSIVFTFANHPVNYFRPNSTKNIISNDDKIKLLEDLGIDIVVIIPFDEYMTKIPAK
                     DFVDEILVKKLKAKKIVIGHDFTFARAKEGNVNLLKSLEHEFGFEVEVIKPIKINDVR
                     VSSTYIRSLVSQGDMANVKEYLGRNYKLEGCVIHSKHLGRTIGFPTANIDLKNNMLVP
                     KRGIYASIVHIGNETYFGATNVGYNPTVNGKTLSIETNILEFDRDIYGENIIVEFLER
                     IRDERKFNSIDDLKNQLYNDTNYVYENYVCKNK"
     gene            19999..20256
                     /gene="rpsO"
                     /locus_tag="KRL67_000728"
     CDS             19999..20256
                     /gene="rpsO"
                     /locus_tag="KRL67_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002843739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HBH1521633.1"
                     /translation="MINKSEIIKEYGRAEGDTGSPEVQIALLTARINELNGHLKTHKK
                     DHHSRRGLLKMVGRRRNLLAYLKEKDLEGYRALIAKLGLRK"
     gene            20496..21347
                     /locus_tag="KRL67_000729"
     CDS             20496..21347
                     /locus_tag="KRL67_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HBH1521634.1"
                     /translation="MKIKLVCDSLCDIPDEISEKDYIEIVPLTVIFNDREYIEGVDIK
                     KEEFYKKVKEIKQIPKTSQATYMEFKEVFDKFVTEGYHIICMTGAANASGTFQSAMIA
                     KNDVNENEKEKIHIFDTRNLSLGSGQYVIKACELLEEGLGFEEIIDELENTRSSVRLL
                     FAPYTLDFLKQSGRVPVATALIGNMLNIKPIFFFDNGEAKLVNKVRGIKNIASKLVDI
                     ILEMNEGSLEGKIVTIGCGDNLHDCEILKDEVNKRIKARRVLFTRGGVSICSHTGPDI
                     LAISCSN"
     gene            21472..23583
                     /gene="pnp"
                     /locus_tag="KRL67_000730"
     CDS             21472..23583
                     /gene="pnp"
                     /locus_tag="KRL67_000730"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HBH1521635.1"
                     /translation="MFEHKIFKMDFAGRELSVEIGKICEMASGSCIVRYSDSMVMVNT
                     TKSAKPRDGIDFFPLSVDYEEKLYSVGKIPGGFLKREGKPSEKAILTSRLIDRPIRPL
                     FPKGFRNDVQVVATVLSVDQDCTPDIVAMIGSSIALSISDIPFNGPTGSVCVGLVDGA
                     FVVNPNAEQREKSSMHLVVSGTKEAIMMVEAGADEVPDEVMLDAILFAHQEIKKVVEF
                     IDGIVAEVGKEKMAVDLYHAGEEITQLVREFATDKMKKAVQTFEKLERMENMDRVKEE
                     TLAHFENTLEDFEDFVGDIEEVLQDIIKEEVRKLIVHENVRPDNRKLEEIRPIWCETG
                     MIPRAHGSAIFTRGQTQVLNVATLGALGDVQKLDGLDEEENKRYMHHYNFPAYSVGEA
                     RPSRGPGRREIGHGALAERALLPVIPSQEEFPYAIRLVSEVLSSNGSTSQASVCGSTL
                     SLLDAGVPIKDMVAGIAMGLIKHDGKVAVLSDIQGMEDHLGDMDFKVAGTEYGITAIQ
                     MDIKIDGIDKEILQRALKQAKEGRIHILGEMRKTISQPKPELSPYAPKIVKMQINPDK
                     IKDVIGPGGKIITKIIDETGVKIDIEQTGEVFISGIEIDMIKKAQELINNIVVEPEVG
                     KTYKGKVSRIMNFGAFVEILPGKEGLLHISHIAHERVAKVEDVLNIGDEVEVKVTEID
                     EKGRVNLSRKVLLPKPEHKNK"
     gene            23849..24592
                     /locus_tag="KRL67_000731"
     CDS             23849..24592
                     /locus_tag="KRL67_000731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HBH1521636.1"
                     /translation="MIMMVLNKSKLKKIIILILVVLLVLSGGVMVLKLFNNKPVFNLF
                     NGVDLPYERGTKDKSGYVALTCNIDLGWETEYVESILETLKKENVKITFNVTGKWAEK
                     NKDELLKIKKQGHEIGNHGYKHLDYSTLSYEENYEQIETSKKIIEEIIGEKTKFFQAP
                     AGSFGPETVKAAKALGYTSIKWDADTIDWKYKDQPEVIIDRMKKKDIKDSSIILMHPT
                     NATTKCIDDIIAIVREKGLKPGKLSDVFK"
     gene            24750..25997
                     /locus_tag="KRL67_000732"
     CDS             24750..25997
                     /locus_tag="KRL67_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HBH1521637.1"
                     /translation="MYKTKILENGLTIIGEEIPYLKSITLGIWINAGSRIEEAQVSGT
                     SHFIEHMMFKGTKNRTSKEIASSIDNLGGQINAFTSKECTCYYVKLIDEHIDTGIDVL
                     SDMILNSKFDKNDIDKERLIILEELKMYEDSPDDLSYDLLVENIYANDGLGMNIIGTK
                     ESLYNITRESMLEYLNKYYIPNNAVISIAGNFNFDDMVEKIKSKFGHWEKKNLSIDIS
                     EAKFNPCFISKNKDTEQVNLAMCLKGIPFENDEEVYSMAVVNNIFGGSISSRLFQKIR
                     EEKGLVYSIYSSQTLYRKCGELGIFASMSTENLQDVYNLIKKEIENIRENYLTEKEIS
                     ESKEQLKGNYILDLESTSSRMMSTGKSMLLSKKVKTTDEILECINNVNINSVKKVVDK
                     VFNIENIGTCIVGRDVEKILHLD"
     gene            26032..26277
                     /locus_tag="KRL67_000733"
     CDS             26032..26277
                     /locus_tag="KRL67_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlmC/YmxH family sporulation protein"
                     /protein_id="HBH1521638.1"
                     /translation="MNLSEIAGKEIVNLVTGERLGVIGECDLILDETTGGILALLIPK
                     EKGFFARRKDKSFLEVPWRNVKKIGNDMIIIEYEGYI"
     gene            26287..27510
                     /gene="dapG"
                     /locus_tag="KRL67_000734"
     CDS             26287..27510
                     /gene="dapG"
                     /locus_tag="KRL67_000734"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HBH1521639.1"
                     /translation="MSILVQKFGGTSVESYEKMNEVCKIVKAYKKNDEELQLVLVVSA
                     MGRKGAPYATDTLINLCNAVNDEPSKRELDLIMSCGEIISGTILANLLDAQGIDSVFF
                     TGQQAGIITSDEYSNAKIKYINPKKVKRALDDGKVVVIAGFQGVTDDGEITTLGRGGS
                     DTSAVAIGKALECETVEIYTDVDGIMTADPRVEPNAKVLSFIDYEEVFQMADKGAKVI
                     HPRAVQLAKSGNITLAIKNTMNPTFEGTKIGSLCRHLEDNIEYEQERDFKVAVANKDS
                     VAQVKIKSAEEVFTEVLNEIEKKLITIDMINFFISEKAFVVEDADIKSLKEILDKFEL
                     DYEVNRDCAKVTLICSRIADEMSGIMSKVVRGLSKAGVSLLQTSDSNMTISCLVSEED
                     MHTAVHAIHQQFYLK"
     gene            27623..28357
                     /locus_tag="KRL67_000735"
     CDS             27623..28357
                     /locus_tag="KRL67_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit"
                     /protein_id="HBH1521640.1"
                     /translation="MKDYREDNERFDKDNCSEESSCEERECIKQFGTNEMPPQPPKDI
                     QCITIIGEIEGHFIGNPQKKATKYEHIIPMLYSIEESNDVKGVLVVLNTVGGDIEAGL
                     AIAELLNSTSKKVVTLVLGGSHSIGVPLATAGDYSFIAPTATMIIHPVRTTGLVIGIN
                     ETFEYFKKMQDRIIQFIIRTSNIKKDVLEKLMHEKDELVSDVGSVLIGKEAVDYGLID
                     EVGGIKEALKKLRELIKESEEEKNND"
     gene            28480..30894
                     /locus_tag="KRL67_000736"
     CDS             28480..30894
                     /locus_tag="KRL67_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HBH1521641.1"
                     /translation="MATKKKKKKKVNTLSFNAEYHNLITIFIGVFLLYSLNSNSMGWI
                     PVLMQNLFKGLFGGLSIAIPFIVIITGLLGFFDGNEYIYRLRKTKLYYIIILFIFVFY
                     GLLNAGTLPVDSPLKGNMFDDVMKLGVSGQGSGLIATTIAYYMSKIFGIAGGWLISIF
                     ALILSVMFIFNISIKDLVSNAKSKSKASKDSNLTFKDKIANMKKSAIDMMTDEVDDTT
                     INKKPGFFKGLMSKGRNNEDEDEDEYFEEENSDGVDDKTIKIVGFNKAEDEYLEILEG
                     TQSMPELDVLKELQKATNENPVVDTKPEKKVDIAKPNLNIEKTQPMSIVAEPVNEDYS
                     NYKKPSIELLNKVNKKSDENGKKKVLKNASLLEKTLSDFGVEAKINQVTVGPTITRYE
                     IQPSPGVKVSKIVNLTDDIALSLAAKSIRIEAPIPGKSAIGIEVPNEEAQMVGVREVL
                     ESEEFNNFNSPLAMGLGKDVAGKIIIGDIGKMPHLLIAGSTGSGKSVCVNTLISSILY
                     KANPDEVKLLLIDPKVVELANYNGIPHLLIPVVTDPKKAANALNWAVTEMNRRYKLFA
                     DAQVKDVTSYNEKAEEKLPKIVIIIDELADLMMASANDVEDYICRLAQMARAAGMHLI
                     VATQRPSVDVITGVIKANIPSRIAFAVSSQTDSRTILDMGGAEKLLGKGDMLFYPLGA
                     AKPVRLQGAFISESESEKVIDFVKSQVKDGIKYEEDIIETISKVNTSKGSDEDEFLSE
                     AIEFVVESGQASASMLQRRFKIGFNRAARLIDSMEERGIIGASEGSKPRKVLISKQDL
                     QNLEGE"
     gene            30897..31955
                     /gene="mnmH"
                     /locus_tag="KRL67_000737"
     CDS             30897..31955
                     /gene="mnmH"
                     /locus_tag="KRL67_000737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-selenouridine(34) synthase MnmH"
                     /protein_id="HBH1521642.1"
                     /translation="MSVIKIEKALKLDKVIFVDVRTEGEYEEDHILNAINMPLFKNNE
                     HNEVGTIYKMQGKHEAIQKGFDYVSYKLKDIYLQAAELALNYDNIVIYCARGGMRSGS
                     IVNLLSSLGVNVYQLEGGYKAYRNFVLEYLRHVMDTKNFVSVHGLTGTGKTDLLHLLD
                     EKGIDVLDLEGIAKNSGSTFGFITFDKKPPSQKNFETKLFESIFFSKENYIFVESESK
                     RVGSVTVPNEIFEAMTREGHHILLECDIENRVDRLCRDYIYDKDEGNILILKECINKF
                     RKRLGNKVVDDYIDLLESGEYKELVKRYLLEYYDPLYMHSVEKYKYDKIINFNDTQKA
                     LEDVIDLYESILEGENEC"
     gene            31949..33283
                     /gene="rimO"
                     /locus_tag="KRL67_000738"
     CDS             31949..33283
                     /gene="rimO"
                     /locus_tag="KRL67_000738"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HBH1521643.1"
                     /translation="MLKIALESLGCSKNLVDAEIMMGILNNKGYKLIGDFEEADAIIV
                     NTCGFIESAKQESIDTIINFAELKKTGNLKLLIVTGCLAQRYSEELKTEIPEIDAIVG
                     TGSYQNIDKILKELSEMHQIVSLNDIEFVFNEDLPRYISTPSYMAYLKIGEGCSNNCT
                     YCIIPKLRGNYRSRKFEDIIKEAKKLAESGVKELVVIAQDTTKYGFDLYGKERLSELL
                     EELAKIDGFKWIRVMYSYPESITEELIQVIKKYDNICSYFDMPIQHASNNILKLMNRK
                     TTKEDILNKINLIRSNIPDAILRTTIIVGFPGETEDDFKQLVDFVEEVKFDRLGAFAY
                     SREEDTPADRLPNHIDEEVKIQRRDTLMMIQQKISEELNDKKIGKTYEVLIEEQIEDN
                     VYTGRTQGDAEEIDSIVYVKSVDNLEVGEFVSVQINDAMEYDLMGDVLYELA"
     gene            33270..33812
                     /gene="pgsA"
                     /locus_tag="KRL67_000739"
     CDS             33270..33812
                     /gene="pgsA"
                     /locus_tag="KRL67_000739"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HBH1521644.1"
                     /translation="MNLPNKLTLFRIFLIPVFVLVMLFNVPNKFLIACIIFIIASITD
                     ALDGKIARKYNLVTDFGKFMDPLADKLLVISALTCMIEDHLVSSWMVIIIVARELTVS
                     ILRAIAAADGKVIAAGNSGKLKTITQMVSIVFLLLGAQFENGLILNIGGVLILIATLL
                     TLYSGWEYLYKNKELFMSSK"
     gene            34100..35146
                     /gene="recA"
                     /locus_tag="KRL67_000740"
     CDS             34100..35146
                     /gene="recA"
                     /locus_tag="KRL67_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HBH1521645.1"
                     /translation="MSVDQEKLKALNEALGKIEKDFGKGSVMKLGEATSMSIDVISTG
                     AIGLDIAIGIGGLPRGRIVEVYGPESSGKTTVALSCVASAQKDGGIAAFIDAEHALDP
                     VYAKALGVDVDNLIISQPDTGEQALEIAEALIRSGAIDIIVIDSVAALVPKAEIDGDM
                     GDSHVGLQARLMSQALRKLTGSIKKSNCVAIFINQLREKVGIMFGNPETTTGGRALKF
                     YSSVRLDVRKIDTIKQGDKVIGSRTRVKVVKNKVAPPFKQAEFDIMYGEGISKIGDLL
                     DIAADVDIVKKSGSWYSYNDTKLGQGRENVKKFLEDNLDLTTEIDEKVRAFYNLNEEH
                     EESGTSVSKEIVEE"
     gene            35322..36863
                     /gene="rny"
                     /locus_tag="KRL67_000741"
     CDS             35322..36863
                     /gene="rny"
                     /locus_tag="KRL67_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HBH1521646.1"
                     /translation="MDVIDSVVMIVIGAAVGIIAGYFVRKNISEAKIGQAENLAKEII
                     DKAHHDSETVQKEKLLEAKEEIHKWRTEAERENRERRTEVQKYEKRVVQKEEVLDRKL
                     QNLESKEVNLSEKLKVVEKKEEEVEVIKTQQLEKLESISGITSDKAKEIILTNAERDV
                     RREMSIMIKEIESQAKEEADKKSREIIGYAIQKCAADHVAETTVTVVSLPNDEMKGRI
                     IGREGRNIRTLETLTGIDLIIDDTPEAVILSGFDPIRREIARIALEKLIADGRIHPAR
                     IEEMVEKARKEVDNIIKEYGEQAAFETSVHGIHPELIRLLGRLNYRTSYGQNVLKHSI
                     EVAHIAGIMAAEIGADIRLAKRAGLLHDIGKAVDHEMEGTHVEIGMDLLRRYKESKEV
                     IHAMSTHHGDYEPQTVEAVLVTAADAISAARPGARRETLEAYIKRLEKLEEIANSYEG
                     VEKSFAIQAGREIRIMVKPEAINDEEIHLLARDMTKKIEDELEYPGQIKVSIIRETRA
                     IEYAK"
BASE COUNT        15048 a         3867 c         7041 g        11120 t
ORIGIN      
        1 gctgatataa acaacaaaga tatttgttta aagcttaata taaaatacat gtttaattta
       61 ataaatataa atagacaact ttatccagct gaaaatagta aaaataatga taaaaaagag
      121 gggatgaaga ataatataga ttcaagtata ttattagctg atgacctttt gagcatttat
      181 agattattaa aaaagataaa aatacatgaa ctttattcaa atattaattt tggaactgaa
      241 aatattggct taacatcttc tgtatatgta ttgataaata ctttgtatgg aaatttattt
      301 aatataattg atgcagaaaa aatgtattta aatgtaaatc cagattttac aaaagatttt
      361 gttttaggaa atataaggat acatataagg ccaagaataa aagctttatt taatattata
      421 ataatgatta ataaaattat gaataagaat aaaggaaata aggaaggtga tagtaatgag
      481 agcaacaggt tcgatacaga gtcttatgga aacaactctt gaaacaatta aaggttctat
      541 agatgcaaat actattatag gagaccctat aaaaacagat actacagtgg ttgtacctat
      601 atcaaaggtt acaattggtt ttggtatagg aggaggagaa tactcaaaag gatatgatga
      661 taaagatagg gaaattgaat taaaaaatga aaaatcagat acgaattttg ctggaggtag
      721 tgcaggggct atatctgtac aaccagtagc atttgtagta gttgaaagtg gagagactag
      781 aataatgagt ttagacagta atatcaatct agtggataat atattatcta taactcctag
      841 agtattggag aaaattcaaa atatatcaca aagtaataag aatcaagata aaaacaatat
      901 gtaagaataa aatattttta aatatgtata tagttttaaa taaagtagtg gggttagtat
      961 aaatcccact attttgttgt gtactttaaa catttatatc taataagatt ttagtttaaa
     1021 acaatttaaa tagataataa agttgatata tagtataatt ataaaaagaa aagaagtatt
     1081 atattataga tatataaacg catttgagat ataagaggga gttataaatc attagttttg
     1141 aggtgaatgt tatgagcgat aaagaattat ctattttgtt gttagaaagt atgaataatt
     1201 ttcataattt aataaagatt attaataacg aaagatataa aaaagataag gttcttacag
     1261 aaagacagtt ctttgcctta gtaaaaataa gaaaacatga taaaatagag cttaaaaatc
     1321 taagtagaga tttacatgta tctacttcta gtttgtgtat actacttaat aaattggttg
     1381 agcaagagta tgtatatagg gaagaagata gtagagatag acgaaatact ttttatggaa
     1441 taactaaaaa tggagaaaag atattggata atgaaatact aaagtttgta tctataataa
     1501 gtgataaaat ggattgctta gatatagata ataaagataa attatttact tctttggaag
     1561 agtctaaaaa tataatagaa caattatttt aaaaaggaga atttattatg aatataaagt
     1621 attctaaaga aatattaaaa agtttagaaa atgctataac agtagtagct ctatttgagg
     1681 gagaagatgt ttctacgaca tttgataaaa taaatatgat aagcagaaat tttaattcta
     1741 aagaaaatag tatagatact gtaacattgg ttgaacatga caatttggta aatgtttgtc
     1801 ttgttggttt tggtaagaga gagttattga atagagaaaa aattagaatc ataggcggga
     1861 atttgtgtaa aaaattaaaa aatatattga aaaaggaagc atataagagt agtgatgtta
     1921 atatactgaa tcttaattta aatagtgatg aaataggttc ttttgtagaa gggatattac
     1981 ttggaaatta taagtttgat aagtataaga caaaaagtaa ggattttgaa agaaaagaag
     2041 ttgatactat gactatattt actgaaaaag aagttgagtg tcaaatagaa aaggctaaaa
     2101 tattagctaa ttcaactata atagctagaa atctagttaa tgaaccatca aacataatat
     2161 atccaaaaac attagcaata gaaacagtta aacttggtgc agagtttggg tttaatgttg
     2221 atgtttatga agaagaaaaa ataaaggcat tgggaatgga tgcttttttc gctgtttcaa
     2281 gaggttcaat taataaacca agattcatag taatgagata ctttggagat aaagaaagta
     2341 cagatatatt aggtctggta ggtaaaggat tgacttatga tactggtgga tattctttaa
     2401 aatcaaatgc ttctatgcta gatatgaaga cagatatggc tggcgctgcg agtgttatag
     2461 gtgctatgtg tgctatatct caatgtaaat taaagaaaaa tgttatagca gtagtggctg
     2521 catgtgagaa tgctttatca ggaggttcat acaaacctgg agatataatt agttctatgg
     2581 caaagaaaac tattgaagtt ttaaatacgg atgcggaagg tagacttaca ttagctgatg
     2641 caatatacta cattataaac aatgaaaaag taacaaaggt ggttgatgta gctacattaa
     2701 ctggtgctgc tttaacattg ttgggcaatg ttgcaactcc tatagtaaca aatgatgatg
     2761 atttttattg tgaattagaa aaagcggcca ctttatcagg ggaaagggta tggaagatgc
     2821 caatttatga tgaatttaga gatatgataa aaggagaaga agcggacctt aaaaacactg
     2881 gtggtaaaaa tgctggatgt ataacagcag gagcttttat tggtgaattt gttggtagta
     2941 ctccatggat acacatggat atagcaggga caagtacatc atcaaaatca attggatata
     3001 aagcaaaggg ggctacagga gaacccgtta gaacattgta ctatttggca gaaatctaaa
     3061 aaacaattat aagatataat ttataaagcc acgtggatat ttttgtagtt taagattatc
     3121 tatgtggctt tattttttat ttaagtaaat tatttactct ttttcttttt ttcaagtata
     3181 aaagtatctc tattaaataa aagagtaggt atgtacataa tactagtaac aaaaataaat
     3241 gtaaaaatag cagttgtgct atgacttaaa aaaccataat agtattgtga gttggtaaat
     3301 gtgtttagta aaaatatgaa tgaaaaataa ataaaacata tagctaagtt atctctaagt
     3361 gcatatctgt taactttaaa ttttctaaca tcaaccatat atatctctgc ataaattatc
     3421 aagcacttag ataagataaa tataaaatat attaagctag gaaattcata gcccaagttt
     3481 ttagaaaaaa tgagttctcc aactgaacaa actaaactcc aagaaaaaaa tatattaaaa
     3541 agatgatact gcttaaaaat tagtatggct atacacagat atatacaaat ataattgatt
     3601 ccaattggca gagagtttag agtactataa ttgcctgaag aaataagtaa taattgctct
     3661 agagccataa ttattacaag taatgtacaa attatcttct caactgtata gctataaggt
     3721 aaaatatttt tggttagttt tggacaataa tataaaaata atccaaatat aaagagactt
     3781 acaaattgtt cactagaaaa aagaaaaatt ttaaacatat aacattctct cctataataa
     3841 atttaatatg tatctatata aatatattca ttatatctca atttaatata taaaaaagat
     3901 aataatagtt agatttttta tgtgaatttt aataggtaaa atatcctatt gttttgtaga
     3961 tatatcctaa ttatgtcaaa aaataaaaaa atttttattt attagagttt ttggaaataa
     4021 taataacaga tattataatt aattaacagt ttttgtaagt atttgacaat agagagggtg
     4081 taacaaatgg gaataataga gtcagcatca aaattggctg agatggttca tttattggct
     4141 gttgaaaagg ggataactga tatagaagca tgggatgaag ctgtgaaaga atacagcaaa
     4201 atttatgaag aaagaagaaa tgaatagaat gataaaaagt gaaaacttga tgataaaaaa
     4261 taactttatt taaagaaatt caatttttat agaaatatta aaaataatat aaataaaaag
     4321 atggtatttc aaatgtaagt aaaaaatcat tttgggatac catccttatt atttatttta
     4381 ataatatata agtaataaat cttacttatc catatttttt atgtaacctc ttttttctag
     4441 cttttttata tgctccttgc gtatttcatc aagattaata ccatattgtt cttctagctt
     4501 aaacatcata gtaacacatg tctgagctac atccattagt tccttagcag ttttctttat
     4561 aatatcaact tcatctaaat cattattttc tccacttaga tttctgaatt tacctataca
     4621 ttcagctaat tcaccttgtt cttcacccat tttgataaat gtagattcta gtgttgggtc
     4681 taaattatta agttctggta aagatatagt ttttagtttc ataaaagctc ctttcaagtt
     4741 attcgtattt atactaaaac atcataacaa aaataatact aaaaaaaaac tttaaattca
     4801 acataaaaac ttgcatatat aataaaaatc gtgaaataaa tctatatttg tcttaataca
     4861 gcttcatata aaacttctcc aataggttta tttattacaa gagaagctga cttatcatag
     4921 accgtatttg ctttattaat aagagcaata taatcacctt tgtaataatc tataaaactt
     4981 gctgctgggt acacagctaa tgatgtacct cctataatca ataaatcagc attacttata
     5041 gcagaaattg ttttagtaat gacatcactg tcaagagctt cctcataaag tactacatct
     5101 ggttttacta tagaaccaca gttgtcacag taaggaatat tacctcccag atttaacata
     5161 gattctaagt caaaaaactt accacattta gtacaataat ttctatgtac agaaccatga
     5221 agttctagca catttttact tccagccatt tgatgaagtc catcaatatt ttgagttatg
     5281 acagctttaa gcttacccat ttcttcgagc tttgctaaag ctatatgagc attattgggt
     5341 tttgcattag gatatattaa tttgtcctta taaaattcaa aaaattcttc tggatatcta
     5401 acaaaaaaag tatgtgaaac taattgttca gcagtaaatt gtttattgag tttttggcta
     5461 aataatccag tggagcttct aaagtctgga atattagatt cagttgaaac acctgctcca
     5521 ccaaaaaata caatattatt gtgattagct attaagtctt ttaagctatt tgcatccata
     5581 gttacacctc caaattatta tatataaatt gtactacata ttaggtcgtt tatgttttat
     5641 taatttaaat aaatatatag tttaataagt taaataaagg gaatataaat aaaatgtata
     5701 cataatataa atagacaata tttactaaaa gaataagttg tcgtataatt atattaaaat
     5761 tgttttagtg tataagaagg gcgggggaag tacgtgaaga aaaaagcagc tttagcaaca
     5821 ttagctatgt tacccttaag tgtggtaaat gcacatgctg atggagatat aggtatagtg
     5881 actataaatt atttaaatgt aagaaatgaa ccaactgccg aaagtagcat agcctttgtt
     5941 gctaaaaaag atgataaagt tttgattaaa gactcttcta atggatggta taaaataaaa
     6001 gctgaatctg gacaagaagg ttgggcttca tcaaaatata tagcaaaatc aaatagtgac
     6061 tctttaagaa cgtctacaaa taaagaaaaa caagttactt caaatagttt aaatatgaga
     6121 aatggagcag gtacaagtta cagagtaata actgtactaa aaaaaggtca aaaagtagaa
     6181 gtaatatcag agagtaatgg atggtctaaa atcaaatatg atggaagatt aggatatgta
     6241 tctagctctt atttaggaga cgtttcaaac tcaactaata aatcaaaaac taaacaagtg
     6301 aatacaactt cactaaatgt aagaagtgga ccaaatacaa gttatggctt gttaggcaag
     6361 ttgccaaaag gaagtaaagt agaagtaata tcagaaagca atggatggtc caaaataaag
     6421 tataatggaa aagatgccta tgtatctagt atgtacttat cggatgtaag tcaaagtaat
     6481 tcagacaatt ctagtcaaag caatgataaa aaaaatactg ataaggttgt aaatacagct
     6541 tctttaaatg taagaagtgg accaggttct acatatagta agttgggaaa agtttataaa
     6601 ggaagtaaag taactgtact atcagaaagt agtggatggg ctaagattag ttttaacaat
     6661 aaagaagcat ttgtagtagg caattattta tctacttcag cagatacttc aaataataac
     6721 tcaaatagta actctgataa cagttctaat aataatggta ataattcttc atcatcaggt
     6781 caagtaaatg gtatgtcagg tataagtggg gctaaaattg attataaatc tttgagttat
     6841 actttggaat ctcatataag taaacaggtt gagaaagcag tgtcaggagg aaatgtgata
     6901 gcgccaagta ataggaaaag cactccaagt cctgaattta gtactttttc agcccaaaga
     6961 acaagctcat ttgtaaatgc aagttcaagt gatatagaat attatttaaa tcctaaaaac
     7021 tttacaaata ctactaaagg tatgatgcag tttttaaaga ttaacagtta tagaggaggt
     7081 atttcagaat caagtcttaa ctcgtatctt aatggtttat cttctagtgt atttaaaaac
     7141 cagggagcag cttttataaa tgctgccaag aagtataata tagatgttgt atatctagta
     7201 tctcatgcta tgtgggaaac tgcttatggt aagtctacac ttgcccaagg tcaaacatta
     7261 acatcttata agggacaacc tcttagtaag ccagttaaag tatacaattt ttttggtata
     7321 ggagcaatag ataaaagtgc taatgtttca ggggcagaag cagcatattc caacggttgg
     7381 actagtgtag aagctacaat agatggctct gcaaagtgga tatcacaaaa ttacgtaaat
     7441 agttctaagt acaatcagaa tactatctac aagatgaagt ggaattatga ttatacctgg
     7501 catcagtatg caactgatgt aaactgggct aatggaattt ctggaattat ggaaaattta
     7561 attggtcttt atggaggagg aagtagtttg gtttttgaag ttcctcaata caagtaatac
     7621 ataaataaaa aatatagact atgggtagtt ttcattttat ttaaattatc tatagtcttt
     7681 ttgtatataa tttataatct gtaaatttaa tgttaacaaa acttttatgt tataatatta
     7741 attctaagta aatataagtg agtaataggt agaaatagat aaaaagtggt aataatatat
     7801 ataaagataa ttttgagaaa ggaggatata tatggaaagc attaaggaat atttggataa
     7861 gttagaaatt aataatagtg gtcttggaaa gcaacttaaa gaagtttata taaatagagt
     7921 agtttatttt aaggaagata agatagttta tttttatctg acttctaaag atattgtttc
     7981 tcatgaactt ttagataaat ttaaagaaga attaatgtat aaactcgatt attttaaaga
     8041 tatgaggatg aagataagat ttactggtct tgaaagaaaa tccaataaag atgttataaa
     8101 aaaatattgg aataatatat tgtatatatt aaagtatctt tgtccatcta ttgcagggtg
     8161 gtataaacaa gtagaatttc tttgtttaga ggaagaatta aaaataaaac ttccaaaagg
     8221 tatattttat gaacgattaa tgaaaaaaaa tgtaacttat gtacttaaaa ccgtgcttag
     8281 cgaagagtta ggattagatt taaatataac aatagaaaaa gctgttgatg aaaaggtaaa
     8341 taaagagaga cttataagaa tcaatgatag agaaatggaa gaaaaaatta gggctttaga
     8401 aattggaaaa gttaataatt gtgaagaaaa tgaagaggaa agctatgtca ttaagtctga
     8461 cgtagatgaa aatttaatct atggagataa tgcaaatgct atgattgaaa atatagttga
     8521 attaaatgca tcttctggaa cagttgcagt tgttggagat atatttgatg ttgacactaa
     8581 agaacttaaa aatggaaaaa tacttatgat agcttcaatt acagattata ctagctcaat
     8641 aagctgtaaa ctttttttaa cagatacgaa taaagatgga gtacttgaaa gtgttaaaaa
     8701 aggagcatac ttaaaaatca agggtgacat agtttatgat acatatcaaa gagaaattag
     8761 tatgatgatt agtgggataa gaaaagaaac tagaatagag agagaagata aatcagagga
     8821 aaaaagagta gagctccatg cacatacaca aatgtcttct atggatgcta tttgttctgt
     8881 taaaaagctt gtagagcgtg cagccaaatg gggtcatcct gctattgcta taacagacca
     8941 tggtgttgta caaggatttc cagatgctat gaatgcaggt aagtcaaata atataaaaat
     9001 actttatgga gtcgaagggt acttggttga agatgattct ctcattatag aggatgcaaa
     9061 tgataaagaa ctttcacaga catttgttgt atttgatata gagacaactg gtttttctaa
     9121 tacaaatgat aaaataactg agattggtgc tgtaaaaata gaaaatttta aaattgtaga
     9181 tagatttagt gagttaataa atcctgaaaa agatatttct tataagatac aggaattgac
     9241 aggaataact aatgaactaa ttaaagacaa gccaaccatt gaagaagttc ttcctaagtt
     9301 tatggaattt gttggagata gtgtattagt agcacataat gcagagtttg ataccggatt
     9361 tatatcgcaa aaatgtaggg aacaaggctt gttatataat aacaaaaaag tagatacact
     9421 tatgttagca agagtaatgt tgccaaactt aaaaagacat agattaaatg tcgttgcaaa
     9481 agaacttggt attcctcttt taaatcacca tagagcagtg gatgatgcgg aggctacagc
     9541 acttatattt aataaatttt tgcagatgct tacagaaaaa ggtgcaaaaa ctttaagtga
     9601 tgtaaataat atacttggaa agattgatta cacaaagctt agcacaaatc acataacctt
     9661 gatagcaaaa aattctatag gtataaaaaa tttatataaa ataatttcag atgcacatgt
     9721 aaatcatttt tttagagcac caagaatcct tagaagtgta ttaaatgaat ataaagaagg
     9781 attaataatt ggttcggctt gtgaagctgg ggtggttttt caagctgtaa agaaaaacgt
     9841 gtctgatgag gaaatgaaaa aaataataga tttatacgat tatattgaag taatgcctat
     9901 agataacaat agatttatga ttgataaagg agaagtaaag gacgaagaag agttaaggga
     9961 gttgaatagg aaactaattg atacagccaa aaaatttgat aaaattccag tagctactgg
    10021 ggatgttcat tttttagata aacatgaagc tatcttaaga aaagttctta aatattctca
    10081 aggttttaaa gtagatgaag aagaaactta tttacatttt agaacaacag atgaaatgtt
    10141 ggaagagttt agttatcttg gagaagaatt ggcatatgaa gttgtagttg aaaatagtaa
    10201 tctaattgcc gatatggttg aagatataaa acctatacca gatgatactt atcctccaat
    10261 tatagagggt tcagatgtgg agttaagaga aatgtgttac aaaaaagcca aacgaatcta
    10321 tggagaccca ataccagaaa tagttcaaaa aagactggat agagaattaa attcaataat
    10381 aagcaatggg tatgcagtta tgtatattat agctcaaaaa ctggttgcaa aatctctttc
    10441 agatggctat ttagttggtt caagaggctc agttggctca tcttttgcag ctactatgag
    10501 tgatataaca gaagttaatc ctcttccagc acattatatt tgtgaaaacg aagactgtaa
    10561 atattcattc ttttatgaaa taggtgaatg gggttcaggt gttgatttac cagacaagga
    10621 ttgtccaaag tgtggaagaa aacttgctaa aaacggtcat gatattccat ttgaggtttt
    10681 cttaggtttt gaaggtgata aggaaccaga catagactta aatttctcag gaaattatca
    10741 gccaacaatt cataaataca cagaagaact atttggagag gggtatgtat atagggccgg
    10801 gactataggt actgtagcag aaaaaacagc ttttggatac gcaagaaaat atgtggaaga
    10861 aaataatata agtgtaccaa atgcagaagt acttaggctt tcaaatggat gtactggagt
    10921 aaaaagaacc tcaggacagc atcctggtgg agttatggtt ataccagact ataaagatgt
    10981 atacgatttt acacctatac agtatccagc aaatgatact tcttgtggtg ttataactac
    11041 tcactttgat tatcattcaa taagtggtag aatacttaag cttgatatac ttgggcacga
    11101 cggtcctacc attataagga tgcttgaaga tatgactgga ataaatataa cagaaattcc
    11161 gttagatgat aaagagacta tgtctctatt tacatctaca gaagcattag gtgttacacc
    11221 agaagaaata aactgtccta taggttgcct tgctgtacca gagtttggaa ctaagtttgt
    11281 acgacaaatg ttacttgaca caaaacctac tacatttgcg gaacttgtac gtatatctgg
    11341 gctttctcat gggacagatg tttgggtaaa taatgcacaa gacttagtta gagaagatat
    11401 agttggatta aaagatgtta tatctacacg agatgacata atgaactatt taatatttaa
    11461 aggattacct cccaaaatga gttttacaat aatggagagt gtaagaaaag gtaaaggtct
    11521 aaaaccagaa cacattgaag aaatgaaaaa aaataatgtt ccagaatggt atataggctc
    11581 atgtaaaaag attaagtaca tgttcccaaa agcacatgct gttgcttatg tcatgacatc
    11641 atttagaatt gcatattgta aagttcacta tccagaagca ttttatgcga cttattttac
    11701 gacaaaggtt gaagattttg atgcagattt aattgttaaa ggattagatg ctataaaatc
    11761 aaaaataaat gaaattgagt cactgggaaa tgatgcaacc acaaaagaaa aaggtatgtt
    11821 gaccgtatta gaagttgctc ttgaaatgta tgctagagga ataaaattat tacccgttga
    11881 tatatataaa tcagatgcga ctgagtttat agttgtagga gaaaaaacat tacttccacc
    11941 aatggcagca atacaaggtc ttggaggaaa tgcggctata aatatccaaa atgaaagaaa
    12001 aaatggtgaa tttatatcaa aagaagattt gagaaagaga acaaaaatat caaaaacggt
    12061 aatagaaact cttactaatc atggttcact tgagaatatg agtgagagaa atcaattgtc
    12121 attgttttaa gttagcccaa aaagttgcaa ataaaagaat tttatgatat catagtatag
    12181 aaaatagtat actttgcaga aactaagagt gagacagctg ttctcactct tttattttaa
    12241 atatattatt aaaaagctgg tttaatctta ttaaatatat tttatttaag attatataaa
    12301 ctggtcactt acaattgaat aggaggaact ttaatgaaaa aaaatttaga agctacaatt
    12361 gaggagattg taactaaaat aacagatgaa catggatttg aaatggttga tgttgaatat
    12421 gtaaaagaag caggagaata ctacttaaga gtatacatag ataaagaaga aggaatatct
    12481 ttaaatgaat gtgaattagt tagtagagaa ttaagtccaa tactagatga aaaagaccct
    12541 ataaaagaga attacttttt agaggtatct tctccaggac ttgatagagc attaaaaaaa
    12601 gacagagatt ttgttagata tcaaggtaga gatgtagatt taaaattata taaaccatta
    12661 aatggatgta agcaatttga aggtgaactt gtaggattaa cagaagataa taatataaaa
    12721 atcattgtaa atggtaagga aatggaattt aatagaaaag atgttgctat tgtcagatta
    12781 gcaataaagt tttagtggta tattaaatag ggaggatata aaaaatgaat catgaattta
    12841 tggaagcatt agatgaatta gtagttgaca gaggtataga taaagaaata cttatagata
    12901 ctatagaaca agctctttta actgcataca agaaaaactt tggttcagca caaaatgtta
    12961 gagttgaatt tgatagagac aagggagata taaaagtatt ttctcaaaga gttgttgttg
    13021 atgaatcaga tttatatgat actttcttag aaatagagtt agcagatgct agagagataa
    13081 atcctaatta tgaattaggt gatattatag aacatgaagt tacacctaaa aactttggaa
    13141 gaattgcagc tcaaacagca aagcaagtag ttgttcaaag aataagggaa gctgagagag
    13201 aaatagtgta taatgaattt atagaaaaag aaaatgaaat agttacagga gaggttgcta
    13261 gagcaaataa aaatgttgtt tatgtaaatc ttggaagaat agaaggtata atgactcaaa
    13321 gtgaacaaat accatttgag aattataaag caggtcaaaa aataaaattc tatatacttg
    13381 aagttaaaaa gactaacaaa ggacctcaaa tagttgtatc aagaagtcat ccaggtctag
    13441 taaagagatt gtttgaagaa gaagttcctg aaatatatga aggaatagtt caaataaaat
    13501 ctgtagcaag agaagcaggg tcaagaacta aaatggcagt aagatctata gatgaaaaaa
    13561 tagacccaat tggagcttgt gttggaccaa aaggacttag agtaaaaaat atagtggatg
    13621 aacttggcga tgagaaaata gatataataa aatatagtga agacccagca gagtttatat
    13681 cagcagcact tagcccatca aaagttgtta gagtagatat agatgaagat gaaaagtctg
    13741 ccttagtaat cgttccagat tatcagttat cgcttgctat aggtaaagaa ggtcaaaatg
    13801 caagactagc agcaaaattg actaactgga aaatagatat taagagcgaa tctcaagcag
    13861 aacaagaaat gttaaatctt gaaaattcca aagaaataga agctgaggat gttgaagctg
    13921 aatttatcga ccaagaagag ttagatactt cagaggataa ttttagtgaa gaagagtaag
    13981 gagggatgat tttgaaaaaa gtaaaaaaag tgcctcaaag aaaatgcata gcatgtcaag
    14041 atagagactc taaaaaagag cttataagaa tagttaaaaa taaagaagga aaaatatttt
    14101 tagacccaac aggaagagcc aatggaagag gagcatatgt atgtaaaagc tctgaatgtc
    14161 taaaaaaagc tataaagagt aaagcattaa ataaagcttt taaaatagat gttcctgatg
    14221 aaatctatga taatctactt atggagttag aagaatatga aaaataataa agaaaaagta
    14281 ctttcgttct tagggctcgc aactcgtgca gggaaaatag tgtctggtga tgattcaact
    14341 ttgcttgacc ttaaaaaagg aaaagtaaat ttgattttaa tagcagaaga tgcatcaaat
    14401 aatactaaaa aactttttaa agataaatca acttttagaa atattccata tctatttttt
    14461 tcaacaaaag aggaaatagg atttgcaata ggaaaatctc caagagcagt tgtaggaata
    14521 aaagatgaaa atttttctaa aaagataatt gaactaatag aaatttaaaa taaaataggg
    14581 ggtgatcttg tgtcaaaaac aagagtatac caaatagcag aagaattaaa tatatcaaat
    14641 gaagagctta taaataagtt agctgaatta gatataaatg taactgacaa agattcagta
    14701 ttagaaggtg aagagttaga attagcactt gaaatgttag gtgaagattt atctcaagaa
    14761 aatggaaatg ttatagaaat agatggaaag ttgacagtac aagtattagc aacgaaatta
    14821 gataaatctc catcagaaat aattatgaag cttatgaaaa tgggaactat ggcaacaata
    14881 aatcaagaga taagttttga gattgctgct ttggctgcca aagactatgg atttgaatta
    14941 acagtagctg aaagtgatga tacagaagct ttagaaatag aagctttaat ggaaattgaa
    15001 gaagataaag aagaggattt aaaacctaga ccaccagttg ttacagttat gggacatgtt
    15061 gaccatggta agacatcttt attagatgca ataagaaaaa ctgatgtgat ttcaggtgaa
    15121 gctggaggaa taactcagca tataggtgct tcagaagtta aaatcaatgg acacaaaata
    15181 gtatttttag atactccagg acatgaggct ttcacatcaa tgagggcaag aggagcacaa
    15241 gttacagata tagcaatatt agtagttgca gcagatgatg gtataatgcc tcaaacagtt
    15301 gaagctataa accatgctaa agcagcaggt gtacctttaa tagttgctat taataaaata
    15361 gataaaccag gtgcaaaccc agataaagtt aaacaggaat tagcagacca aggattatta
    15421 gttgaagact ggggtggaga agtaatagca gttccagtat cagctaagaa gaaagaagga
    15481 atagatacac ttttagaaat ggtattattg gttgctgaaa tggaagaact aagagctaat
    15541 ccaaataaaa gagcagtggg aactgttata gaagctgagc ttgataaagg aagagggcca
    15601 gttgcaacag tacttgttca aggtggtact ttaacagtag gtgacccaat cgttgcaggt
    15661 gttgcatgtg gtaaagttag agcaatgata aatgcaaaag gtaagagagt aaaaacagca
    15721 ggtccatcta cagcagttga gatattaggt ctatcagaag ttccacaagg aggagaccaa
    15781 tttgtcgaag ttccaactga taagattgct agaagtgtag cagcaagaag acaacaaata
    15841 gttagagatg aaatgttaaa atcaactcaa agattatcac tagatgctct atttagtcaa
    15901 atgagtgaag gttctataaa agaccttaat atagttataa aagcagatgt acaaggttca
    15961 gtacaggcag ttaaacaatc tttagaaaaa ttatcaaatg aagaagttca agttaaagta
    16021 atacatggtg gtgttggagc tgtaacagaa tcagacatat tacttgcagc agcttctaac
    16081 gcaataataa taggattcaa tgtaagacca gttccaggtg cagaatctct aggagaaaaa
    16141 gaaaatgttg atataagaac atatactata atatacaagg caatagaaga tatacaagct
    16201 gctatgactg gtatgttaga ccctgaatat gttgatgaag aaacaggaaa agctgaaata
    16261 agagaaatat ataaaatatc tggtgttgga acagtagctg gatgttatgt gactaatggt
    16321 aagatattta gaaattgtaa aataagatta gttagagata gcataataat acatgaaggt
    16381 gagcttgcag cacttaagag atttaaagat gatgtaaaag aagttaattc tggatacgaa
    16441 tgtggtatga gttttgtaaa ttataacgac ataaaagaag gagatatagt agaagcttat
    16501 ataactaaag aagtagaaag aaaattataa attcgtatca aagaaaatca gttagtaaag
    16561 aaggtcgatg gtatggcatc atataataga acaagaagga tagcagaaga aataagaaaa
    16621 gtcgtaagta ctatgcttat aaatggagta aaagacccta gaataacatc tatggtgagt
    16681 gtaactgatg tagaagttac aaatgaccta agatatgctt atgtatatgt aagtattcta
    16741 ggtggagatg aagaatccac actaacaggt cttaaaagtg ctggaggata tataagaaga
    16801 gaagtaggaa aaaatataaa acttagatat attcctgaga tagtatttaa acttgatgat
    16861 tcaatagaaa aaggaatgta tatggatagc cttataaaaa gagttaatga aaaaaatgct
    16921 caacaaaatg aggatgaaaa ttatgatgaa taatatggat agtattataa actatattta
    16981 tgagggtgac tattttatag ttacctctca tatcagtcca gatggagaca atattggttc
    17041 tacactttca atgtattata cattgaagaa tttaggaaaa aatgtatatt atgtacttga
    17101 tgatgaagct ccattaaatt taaggttttt ggttgaaggt ataaaaatct ataagtctaa
    17161 tgaatttaat atgaagaact attctctaat tgcacttgat tgtggggata agatgagaat
    17221 atgtgtgagt gatgaaatca aaaataatgc atcaaaatta atctgtattg accatcatgc
    17281 tagtaatgat tcatatggag atttaaatta tgtagatatt gatgcatctt ctacatgtga
    17341 actagtttat aatttattag ttagatttca gcaaacaaaa gatattaata ttataaatga
    17401 agatatagca acatgtttat atacaggtct tgtaactgat actggaaact tttcgtattc
    17461 aaatgtacat gcaagtagtt ttgaaatggc aaaaaattta cttgttttag gtgcacaaaa
    17521 aaatactata atacaaaata tatatcaaag caattcttct gattattata aattattagg
    17581 agaagctcta aaaggtttgg agatatttaa ttcaaagata tctagtatag ttttaactca
    17641 agacatgatg aatagaaata atattagctt taatgatgtt gatggaataa caacatatac
    17701 aagagatata aaaggtattg aagttggaat tttatttaaa gagaaaaaac aaaatgaaat
    17761 taaagtgagt tttaggtcaa aaaattatgt tgatgtaagt gaaatagcca agttatttgg
    17821 cggtggtggt catgtaaggg ctgctggatg tactattaga gatagtattg ataatgcaaa
    17881 gaaaatggta ctagaggcag tgttaaaatc aatttaaggt gacagtaaaa tgaataagat
    17941 tataagtata ttgaaaccaa caggtatgac atctcatgat gttgtaagta gagtgagaaa
    18001 gattttaaat ataaaaaaag ttggtcatac aggtactctt gacccagatg cttcaggtgt
    18061 tttaccaatc tgtataggga aagccacaaa agtatgtgaa gttattttaa acaaagataa
    18121 atcgtatata tgtgagctta ctttaggtat aagtacagat acttatgatg cctcaggtga
    18181 aattttaaaa aaagttgatg attttaaatt tagtaatgaa gacatagaaa gagcttttga
    18241 tacacaaaga ggagagataa atcaacttcc tccaatttat tcagctttaa aggtaaatgg
    18301 aaagagaatg tgtgatttag taagaagtgg tagacaatct gaaattactt taaaaacaag
    18361 aagagtgaat ataaaagaca taaaaatact aagtataaaa ggtaataaag taatgtttta
    18421 tgttgagtgt tctaaaggga cttatgtgag aagtatttgc catgatatag gagaatatct
    18481 aggttgtgga gctcatatgt cgtttttgaa tagaacatct tctggtaaat ttgacttaga
    18541 taattcaata acattagaag aattagaatt attttatgaa aataaaactt tagacaaata
    18601 cttgtatgat atagattatg tacttgattc gtttaattat gttgtattaa accctaatgc
    18661 tataaaatat tatagtaatg gtggctcaat agatgataaa aggtttttaa aaaataattt
    18721 cgataaagat gatgaatttg taagagtata cagtacagat aatttcttag gattaggaaa
    18781 gttgtcaaaa cacaataata caataagtgt taaaagtgat aagatgttca tataaccaat
    18841 gtatttaaaa aaacaacaaa agagatagta ctataataac ttctatagtc tatctctttt
    18901 acttatgcta taatagtttg tgaataagtt aagagtaaat tatattttag cggggaacat
    18961 ttattatggt gattataaaa tctataaaag aaatagcaaa cataaaagaa agtgtcatta
    19021 ccataggaaa ctttgatggt gtacacaaag gacatcaagt tcttatagga aaaacagttg
    19081 aacatgcaaa aaaagaaaat atacagagta ttgtatttac atttgcaaat catcctgtaa
    19141 attactttag accaaactca acaaaaaata ttataagcaa tgatgataaa ataaaattat
    19201 tagaagattt gggaatagat atagttgtaa tcataccatt tgatgagtat atgactaaaa
    19261 tacctgcaaa ggatttcgta gatgagatac tagtaaaaaa attaaaagct aaaaagatag
    19321 taattggaca tgactttaca tttgcaaggg caaaagaagg caatgttaat ctattaaaaa
    19381 gcttagaaca tgaatttggt tttgaggtag aagtaataaa gcccatcaaa atcaatgatg
    19441 taagagtgag tagtacttat attagaagtt tagtatccca gggtgatatg gctaatgtca
    19501 aggaatattt gggtagaaat tacaagttag aaggttgtgt tatacattct aaacaccttg
    19561 gaagaactat aggattccca acagcaaata ttgaccttaa gaataatatg ctcgttccaa
    19621 agagaggtat ttatgcttct attgtacata ttggaaatga gacttatttt ggtgctacaa
    19681 atgtaggtta taacccaaca gttaatggaa aaactttgtc tattgaaaca aatattttag
    19741 agtttgatag agatatttat ggagaaaata taattgttga atttttggaa agaataagag
    19801 atgaaagaaa gtttaattca atagatgact taaaaaatca attgtataat gatacaaatt
    19861 atgtgtatga aaactatgtt tgcaaaaaca agtaatgtat gataaaatga ttaaggaaaa
    19921 taaccattac tcggcatttg aatactccaa tcaatgctta gtaataggcg tttctaaaaa
    19981 aatacggagg taatctaaat gataaacaaa tcagaaataa taaaagaata tggaagagct
    20041 gaaggagata ctggttctcc agaagtacaa atagctttat taacagctag aataaacgag
    20101 ttaaatggac atttaaaaac tcataagaaa gaccaccatt caagaagagg actattaaaa
    20161 atggttggta gaagaagaaa cttattagct tacttaaagg aaaaagatct tgaaggatat
    20221 agagcattaa tagctaaatt aggattaaga aagtaatttc attctttata aaaaagcagg
    20281 tatagtgtat acctgctttt ttatagttaa ttcattataa agtaaaataa ttaataataa
    20341 aagtagttta tatgttataa aaaattataa aatgaataat attgggtaag aatattaaaa
    20401 tagtatacaa atacataatc aatttaaaag gaaagacagg ataaatatag aaattttata
    20461 acaggataat tagttggaat aaggaggata ttattttgaa aataaagtta gtatgcgata
    20521 gtttgtgcga tattccagat gaaatatcag aaaaagatta tatagagata gttccactta
    20581 cagttatttt taatgatagg gaatatatag aaggagtaga tataaagaaa gaagagtttt
    20641 ataaaaaagt aaaagaaatt aagcaaatac ctaaaacttc acaagctaca tatatggagt
    20701 ttaaagaagt gtttgataag ttcgtaacag aaggttatca tataatctgt atgacagggg
    20761 ctgcaaacgc atcaggaact tttcaaagtg ctatgatagc taaaaatgat gtaaatgaaa
    20821 atgaaaaaga aaaaatacat atatttgata ctagaaattt atctctaggt agtggacaat
    20881 atgttataaa ggcatgtgaa cttcttgaag aaggactggg atttgaagaa attatagatg
    20941 aattagaaaa tactcgtagt agtgtaagat tactttttgc accatataca cttgattttt
    21001 taaagcagag tggaagagtt ccagttgcaa ctgctttgat aggaaatatg ttaaatataa
    21061 agcctatatt tttctttgat aatggggaag ctaagttggt aaataaggtt agaggaataa
    21121 agaatatagc aagtaaattg gtcgatataa ttttagaaat gaatgaaggg tctttagaag
    21181 gaaaaatcgt cacaataggc tgtggagata atttacatga ttgtgaaata ttaaaagatg
    21241 aagtaaataa aagaataaaa gcaagaagag tattatttac tcgaggagga gtttctattt
    21301 gctctcatac agggccagat atattggcta taagttgttc taattagtag taatataagg
    21361 tttttgcata tatgtgtgaa gataatttta tattgttaat ttatagtatt tttgataata
    21421 tagtaaatag agtgatatac gtgtttttta ataagaggag gacaagcatt aatgtttgaa
    21481 cataaaatat ttaaaatgga ttttgcagga agagaacttt ctgtagaaat aggaaagatt
    21541 tgtgaaatgg ccagtggtag ttgtatagtt cgatacagtg acagtatggt aatggtaaat
    21601 acgactaaat cagccaaacc tagagacgga atagactttt ttcctttaag tgtggattat
    21661 gaagaaaaat tatactctgt aggtaagata cctggaggtt ttttaaagag agaaggtaag
    21721 ccttcagaaa aagctatact tacatcaaga ttgatagata gaccaataag acctttattc
    21781 ccaaaaggat ttagaaatga tgttcaggta gttgcaacag ttctatcagt agaccaagat
    21841 tgcacaccag atatagtagc aatgataggt tcttcaatag ccctttctat atcagacata
    21901 ccatttaatg gaccaacagg ttcagtatgt gttggccttg tagatggtgc atttgttgta
    21961 aatccaaacg cagagcaaag agaaaaaagt tcaatgcatc tagttgtttc aggtacaaag
    22021 gaagcaataa tgatggttga agccggagct gatgaagttc cagatgaggt aatgcttgat
    22081 gctattttat ttgctcatca agaaataaaa aaagtagttg aatttataga tggtatagtt
    22141 gcagaggttg gaaaagaaaa aatggctgtt gatttatacc atgcaggaga agaaataact
    22201 caattggttc gtgaatttgc aactgataag atgaaaaaag cagttcagac ttttgaaaag
    22261 ttagagagaa tggaaaatat ggatagagtt aaagaagaaa ctttagctca ctttgaaaat
    22321 actttagaag attttgaaga ttttgttgga gatatagaag aagtattaca agatataata
    22381 aaagaagaag taagaaaact tatagtacat gaaaatgtaa gaccagataa tagaaaactt
    22441 gaagaaataa gacctatatg gtgtgaaact ggcatgatac ctagagctca tggttcagcc
    22501 atatttacta gaggacaaac tcaagtactt aatgttgcta ctttaggtgc tttaggagat
    22561 gttcaaaaat tagatgggtt agatgaagaa gaaaacaaga gatatatgca tcattataat
    22621 ttccctgcat atagtgttgg agaagctaga ccatcaagag gaccaggaag aagagaaata
    22681 ggacatggtg ctctagcaga aagagcttta ttaccagtaa taccatctca agaagaattt
    22741 ccttatgcta ttagattagt ttctgaagta ttaagttcaa atggttctac atctcaagca
    22801 agtgtatgtg gctcaacatt atctttgctt gatgcaggtg tacctataaa agatatggtt
    22861 gcaggtatag caatgggact tataaagcat gatggtaagg ttgcagtgct ttcagatata
    22921 caaggtatgg aagaccattt aggtgatatg gactttaaag tagctggaac agagtatggt
    22981 ataacagcaa tacaaatgga tataaaaatt gatggtatag ataaagaaat tctacaacgt
    23041 gctttaaaac aagctaaaga aggtagaatc catatactag gagagatgag aaaaactata
    23101 tctcagccaa aaccagaact ttcaccatat gcacctaaga ttgttaaaat gcaaataaat
    23161 cctgacaaga taaaagatgt aataggacct ggtggaaaga taataactaa gataatagac
    23221 gaaactggag ttaagataga tattgaacaa actggggaag tatttatatc aggtattgag
    23281 attgatatga ttaagaaagc tcaagaactt ataaataata tagtagttga accagaagta
    23341 ggaaaaactt ataaaggtaa agtaagtaga ataatgaact ttggcgcatt tgttgagata
    23401 cttccaggaa aagaaggatt acttcatata tctcatatag ctcatgaaag agttgccaaa
    23461 gttgaagatg tattgaatat aggggatgaa gttgaagtta aagttacaga gattgatgaa
    23521 aaaggaagag ttaatttatc aagaaaagtt cttttaccaa aaccagaaca taaaaataaa
    23581 taacttatta tataattaaa agcaaagtct taggattttg cttttttaaa tacaaaaaag
    23641 tagataaatt taaactaata gttatagatg gcttatagtc ctttttctgt aaggttttac
    23701 tcttataaag aagctattta aaagttgatt aatagaaagt tttattgata tatatagaat
    23761 cacttaagtt atatttagtg cataaacaga gcttattttt aatagtatta aataagaaac
    23821 aatttgtaca taattagggg gagttttaat gataatgatg gtacttaata agagtaagct
    23881 caaaaaaatc ataatactaa ttttagtagt gttattagta ttatcaggag gggtaatggt
    23941 attaaagtta tttaacaaca agccagtatt taatttattt aatggtgtag atttaccata
    24001 tgagagaggt acaaaggata aaagtggata tgttgcatta acctgtaata tagaccttgg
    24061 atgggaaaca gagtacgtag aatccatttt agaaacactt aaaaaagaaa atgtaaagat
    24121 tacatttaat gtaactggaa aatgggcaga aaagaataag gatgaattat taaagataaa
    24181 aaaacaagga catgaaatcg gtaatcatgg atacaaacat ttagattatt ctacattatc
    24241 ctatgaagaa aattatgagc aaatagagac ctctaaaaag ataattgaag aaattatagg
    24301 tgaaaaaact aaattttttc aagcaccagc aggttctttt ggtccagaaa cagtaaaggc
    24361 tgctaaagca ttagggtata cttctatcaa atgggatgcc gatactatag attggaaata
    24421 caaagaccaa ccagaggtaa ttattgatag aatgaagaaa aaagacatta aagatagcag
    24481 tataatctta atgcatccaa ccaacgcaac gacaaaatgt atagatgata ttatagctat
    24541 tgtaagagaa aaaggactaa aaccaggaaa acttagtgat gtatttaagt agacaaagta
    24601 taaatatttg gttaagattg tataagttgt agttgaatta attaaaagac tgacttataa
    24661 tgtaatacat ataaaaaata aatataatta aaacatagtg ttttcaataa tccaatatga
    24721 aacaaagaat aaattgttag gagaatgtaa tgtataagac aaaaatactg gaaaatgggc
    24781 ttacaataat tggtgaagaa ataccatatc taaagtcaat aacattaggt atttggataa
    24841 atgcaggctc taggatagaa gaagcacagg tgagtggaac ttctcatttt atagagcata
    24901 tgatgtttaa aggaaccaaa aatagaacat caaaagagat tgctagttca atagataatt
    24961 tgggtggtca aataaatgca tttacaagta aagaatgtac atgttattat gtaaaactaa
    25021 tagatgagca tatagataca ggaattgatg tattgagtga tatgatacta aattctaaat
    25081 ttgataaaaa tgatatagat aaggaaagac ttataatatt ggaagaactt aagatgtatg
    25141 aagactctcc agatgactta tcttatgatt tattagtgga aaatatatat gctaatgatg
    25201 gattaggaat gaatataatt gggacaaaag aatctctata caatataact agagaatcca
    25261 tgttagaata cttaaataag tattatattc caaataacgc tgttatttct atagctggaa
    25321 actttaactt tgatgatatg gtagagaaaa tcaaatcaaa atttggacac tgggaaaaga
    25381 aaaatttgag tatagacata agtgaggcta agtttaatcc ttgctttata tcaaaaaata
    25441 aagatactga acaggtgaac ttagctatgt gcctaaaagg tattcctttt gaaaatgatg
    25501 aagaagtcta ctcaatggca gtggtaaata atattttcgg tggaagtata agctcaaggc
    25561 tttttcagaa aataagggaa gaaaaaggtt tagtatattc aatatactca tcacagacat
    25621 tatataggaa atgtggtgaa ttaggtatat ttgcaagtat gagtacagaa aatttacaag
    25681 atgtatataa tttaataaag aaagaaattg aaaatataag agaaaactat cttacagaaa
    25741 aagaaatatc tgaaagtaag gaacagttaa aaggaaatta tatattggat ttagaaagta
    25801 ctagcagtag aatgatgtct actggtaaat ctatgttact tagcaaaaaa gtaaaaacta
    25861 cagatgaaat acttgaatgt ataaataatg ttaatattaa ttcagtaaag aaggttgttg
    25921 ataaggtatt caatatcgaa aatattggta catgtatagt tggaagagat gtagaaaaga
    25981 ttctacactt ggattaacat tgaaaaaagt aaatctctgg aggtataaaa tatgaattta
    26041 tctgaaatag caggtaagga aatagttaac ttggtcacag gagaaagatt gggggtcatt
    26101 ggagaatgtg accttatact agatgaaact acaggcggca tattagcgct tttaattcca
    26161 aaagaaaaag gattttttgc cagaagaaaa gataagtcat ttttagaagt accatggaga
    26221 aatgtaaaaa aaataggtaa tgacatgata attatagaat atgagggata tatttaggag
    26281 gaaaaaatga gtattttagt gcaaaagttt ggtgggacat cagtagaatc atatgaaaaa
    26341 atgaatgaag tatgtaagat agtaaaagca tacaaaaaaa atgatgaaga attacaatta
    26401 gtattagtag tttcagcaat gggaagaaaa ggagctccat atgctacaga tacacttata
    26461 aatttgtgta acgcagtaaa tgatgagcca agtaaaagag aacttgattt aataatgtct
    26521 tgtggggaaa taatatcagg aacaattctt gctaatttat tggatgcaca ggggatagat
    26581 tctgttttct ttactggtca gcaagcaggt ataattacaa gtgatgaata ttctaatgca
    26641 aagataaagt atataaatcc taaaaaggta aaaagagctt tagatgatgg taaggttgta
    26701 gttatagctg gattccaggg ggtaacagat gatggagaaa taactactct tggaagagga
    26761 ggaagcgata catcagcagt tgctattggt aaagctttag aatgtgaaac agttgaaatt
    26821 tacactgatg tggatggtat aatgactgca gaccctagag tagaaccaaa cgctaaagta
    26881 ttaagtttta tagattatga ggaagtattc caaatggctg acaaaggtgc aaaagtaata
    26941 catccaagag cagtacagtt ggctaaaagt ggcaatataa ctttagccat aaagaataca
    27001 atgaatccaa cttttgaagg tacaaaaata ggttctttat gtagacactt agaagataat
    27061 atagaatatg aacaagaaag agattttaag gttgctgttg caaataaaga tagtgtagcg
    27121 caagttaaga ttaagtcagc agaagaagtg tttacagaag tattaaatga gatagaaaag
    27181 aaacttataa ctatagatat gataaatttc ttcatatcag aaaaagcatt tgttgtagaa
    27241 gatgctgata taaaatcatt aaaggaaata ctagataaat ttgaattgga ttatgaagta
    27301 aatagagact gtgctaaggt aacattgata tgttcaagaa ttgctgatga aatgtctggt
    27361 atcatgtcaa aggtagttag aggattatca aaagcagggg tatctctttt acaaacatca
    27421 gactcaaata tgactatatc ttgtttagtt agtgaagaag atatgcatac agcagtacat
    27481 gctatacacc aacaatttta tttaaaataa actgtataat acaattattt tttaatcaaa
    27541 tttgagaatt tcatatatat cttaatatat ttgatttatt tggtaaaact aaattaaata
    27601 taattttgat aggagttttt ttatgaaaga ttatagagaa gataatgaaa gatttgataa
    27661 agataattgt agtgaagaat ctagttgcga agagagagaa tgtataaagc aatttggtac
    27721 aaatgaaatg ccacctcaac ctcctaaaga tattcaatgt ataactataa ttggagaaat
    27781 tgagggacat tttataggaa atcctcaaaa aaaagctaca aagtatgagc atataattcc
    27841 aatgctatac tcaattgagg aaagtaatga tgttaaagga gtattagttg ttttaaatac
    27901 tgttggagga gatattgaag caggtttagc tattgcagaa cttttaaaca gtacttctaa
    27961 aaaagtagtt actcttgttc taggaggaag tcatagtata ggtgttcccc ttgctacagc
    28021 gggagattac tcatttatag caccaacagc aaccatgatt atacacccag tgcgaactac
    28081 tggtcttgtg attggtataa atgagacttt tgaatatttt aaaaagatgc aagatagaat
    28141 aattcaattt ataataagaa catcaaatat caaaaaagat gtacttgaaa aacttatgca
    28201 tgaaaaagat gaacttgtaa gtgatgttgg tagtgtatta ataggaaaag aagctgttga
    28261 ttatggtctt atagatgaag ttggaggaat aaaagaagca cttaaaaaat taagagagct
    28321 tataaaagaa tctgaggaag aaaaaaataa tgattaaaat ttgtttaaat aataaaattt
    28381 atgatataat gatgtaataa agtaggagtg ttagtgttaa ggcattagca ctttttgctg
    28441 tgaaggggta aaacttgcta aaaataagga gtgatattaa tggccacgaa aaagaagaaa
    28501 aaaaagaagg taaatacctt aagttttaat gcagaatatc ataatttaat aacaatattt
    28561 ataggtgtat ttttattata tagtttaaat tctaattcta tgggatggat acctgtgtta
    28621 atgcaaaatc tttttaaggg actatttgga gggctttcaa tagcaatacc atttatagta
    28681 attataacag gacttttagg attttttgat ggaaatgaat atatatatag gctaagaaag
    28741 actaaactat actacataat tattttattt atatttgtat tttatggatt attaaatgca
    28801 ggaactcttc ctgtagatag cccactaaaa ggtaacatgt ttgatgatgt tatgaaattg
    28861 ggcgtaagtg gacaaggtag tggtttgata gcaacaacta ttgcgtatta tatgagtaaa
    28921 atatttggta tagcaggcgg atggttaata agtatcttcg cgcttatact ttctgtaatg
    28981 tttatattta atatttctat aaaagatttg gtgtcaaatg caaaatcaaa atcgaaagca
    29041 tcaaaagaca gcaatttaac gtttaaagat aaaattgcaa atatgaaaaa atctgcaata
    29101 gatatgatga ctgacgaagt agatgatact actataaata aaaagccagg tttctttaaa
    29161 gggttaatgt caaaaggtcg taataatgaa gatgaagatg aagatgaata ttttgaagaa
    29221 gaaaatagtg atggtgtaga tgataaaact ataaaaatag ttggatttaa taaggcagaa
    29281 gatgagtatt tagaaatatt agaaggaact caaagtatgc cagagttaga tgttttaaaa
    29341 gaattacaaa aagctactaa tgaaaatcca gttgtagata ctaaaccaga aaaaaaagtt
    29401 gatatagcta aacctaattt aaatatagaa aaaactcaac ctatgagtat tgtagcagag
    29461 ccagttaatg aggattattc aaactataaa aagcctagca ttgagctttt aaataaagta
    29521 aataagaagt ctgatgaaaa tggtaaaaag aaagtgctga aaaatgcttc tcttttagaa
    29581 aagacactat cagattttgg agtggaagca aaaattaatc aagttacagt tggacctact
    29641 ataacaagat atgagataca accaagtcca ggagtcaagg taagtaaaat cgtaaattta
    29701 actgatgata tagctttaag tttagcagct aaaagtataa gaattgaagc accaattcct
    29761 ggtaaaagtg caattggaat agaagttcca aatgaagaag ctcaaatggt tggtgtgaga
    29821 gaagttctag aaagtgaaga atttaataat tttaactctc ctcttgcaat gggactggga
    29881 aaagacgtag caggaaaaat tataattgga gatataggta agatgccaca cttactaata
    29941 gctggttcta caggttcagg aaagagtgtg tgtgtaaata cattaataag tagtatttta
    30001 tataaggcaa atcctgatga agtaaaatta ctattaatag acccaaaagt agttgaactt
    30061 gcaaattata atggaatacc acatctattg ataccagtag ttactgaccc taaaaaagct
    30121 gcaaatgctt taaattgggc agttacagag atgaatagaa ggtataaatt atttgcagat
    30181 gctcaagtaa aagatgtaac aagttataat gagaaagcgg aagaaaaact tcctaaaata
    30241 gtaataataa tagatgagtt agcagattta atgatggcaa gtgcaaatga tgtagaagat
    30301 tatatttgta gattagctca aatggcaaga gctgctggaa tgcacttgat tgtagcaact
    30361 caaagaccat ctgttgacgt tataacaggg gtaataaagg ctaatatacc atctagaata
    30421 gcatttgcag tgtcatctca aactgattca agaacaatcc ttgatatggg gggtgcagaa
    30481 aagcttctag gtaaaggtga tatgttattc tatccacttg gtgcagcaaa gccagttaga
    30541 cttcaagggg cattcatatc agagagtgag tcggaaaaag taatcgattt tgtaaaaagt
    30601 caggttaaag atggaataaa atatgaagaa gatataattg aaactatatc taaggttaat
    30661 acttcaaagg gtagtgatga ggatgagttt ttgagtgaag cgatagaatt tgtcgtggaa
    30721 agtggtcaag catctgcctc tatgctacag cgaagattta aaataggatt caatagagca
    30781 gctagactta tagattctat ggaagaaaga ggaataattg gagcaagcga gggaagtaaa
    30841 ccgagaaagg tattaataag caaacaggat ttgcagaatt tagaaggtga atagaattgt
    30901 cagttataaa aatagaaaaa gcacttaagc tagataaagt aatatttgta gatgtgagaa
    30961 ctgaaggaga atatgaagaa gaccatatat tgaatgcaat caatatgcct ctttttaaaa
    31021 ataatgaaca taatgaagtt ggaacaattt ataaaatgca gggaaaacat gaagcaatac
    31081 aaaaagggtt tgactatgta tcgtataaat taaaggatat atatttacaa gcagcagaac
    31141 ttgcattaaa ttatgataat attgtcatat attgtgcaag aggtggaatg agaagtggca
    31201 gtatagtaaa tttattatca tcattaggtg taaatgtata tcaacttgaa ggtggatata
    31261 aggcttatag aaattttgtt ttagagtatt taaggcatgt aatggataca aaaaactttg
    31321 tatctgttca tggattgact ggtacaggaa agactgattt gttacattta ttagatgaaa
    31381 aaggaataga tgtactggat ttagaaggga tagctaaaaa cagtggttct acctttggct
    31441 ttataacttt tgataaaaag ccaccatctc aaaagaactt tgaaacaaaa ttatttgaaa
    31501 gtatattttt ttcgaaagag aattatatat ttgtcgagag tgaaagcaaa cgagttggaa
    31561 gtgttacagt tccaaatgaa attttcgagg caatgacacg agaaggacat catatattat
    31621 tagaatgtga tattgaaaat agagttgata gactttgtag agactatatt tatgataaag
    31681 atgaagggaa tatattgatt ttaaaagaat gtataaacaa gtttagaaaa agattgggta
    31741 ataaagtagt agatgattat attgatttat tagaatcagg tgaatataaa gaacttgtaa
    31801 aaagatatct tttagaatac tatgacccac tttatatgca ttcagtagag aaatataagt
    31861 atgataagat tataaatttt aatgatacac aaaaagcttt agaagatgtt atagacttat
    31921 atgagagtat attggaagga gaaaatgaat gttaaaaata gcattggaat cattaggatg
    31981 ctcaaaaaat ttagtagatg cagaaataat gatgggaatt ttaaataaca aaggctataa
    32041 attaattgga gattttgaag aagctgatgc aattatagta aatacatgtg gatttataga
    32101 gtcagctaaa caagaatcta tagatacgat aataaacttt gcagaattaa aaaaaactgg
    32161 caatttaaag ttgttgatag taacaggatg tctagcccaa aggtattctg aagaattaaa
    32221 aactgaaatt cctgaaatag atgctatagt aggtacagga agctatcaaa atatagataa
    32281 aatacttaaa gaattaagtg aaatgcatca aattgtaagc cttaatgata tagagtttgt
    32341 atttaatgaa gatttaccaa gatatatatc tacacctagc tatatggcct atttaaaaat
    32401 tggagaaggg tgctccaata attgtactta ttgtataata ccaaaactaa gaggtaacta
    32461 taggagtaga aaatttgaag atataattaa agaagctaag aaattagctg aatctggagt
    32521 aaaagaactt gtggttatag cacaggatac gacaaagtat ggttttgatt tatacggaaa
    32581 agaaagatta tcagaacttt tagaagaact tgcaaagata gatggattta agtggataag
    32641 ggttatgtat tcatatccag aatctataac agaggaattg atacaagtta taaagaaata
    32701 tgataatata tgtagttact ttgatatgcc aatacagcat gcaagtaaca atattttaaa
    32761 attaatgaat agaaaaacta ctaaagaaga tatattaaat aaaatcaatt tgataagaag
    32821 taatatacca gatgctatac ttagaactac gataatagta gggtttcctg gtgaaacaga
    32881 ggatgacttc aaacaacttg tggactttgt tgaagaggtt aaatttgata gacttggtgc
    32941 atttgcatat tcaagagaag aagatacacc agcagataga ttgccaaacc atatagatga
    33001 agaagtaaaa atacaaagaa gagatacttt aatgatgatt caacaaaaaa tatctgaaga
    33061 acttaatgac aaaaagatag gaaaaactta tgaggtgtta atagaagaac agatagaaga
    33121 taatgtatat acaggaagaa cacaaggtga tgcggaagaa attgacagta tagtgtatgt
    33181 gaaaagtgta gacaatcttg aagtaggaga atttgtaagt gttcaaataa atgatgcaat
    33241 ggaatacgat ttaatgggag atgttttata tgaacttgcc taacaagtta actttgttta
    33301 gaatattttt aataccagta tttgttttag ttatgttgtt caatgtacca aataaatttt
    33361 taatagcttg tattattttt ataatagctt caattactga tgctttagat ggaaaaatag
    33421 ctagaaaata caatctagtc actgactttg gaaaatttat ggacccatta gcagataagc
    33481 tattggttat ttcagcactt acatgtatga tagaagacca tcttgtaagt agttggatgg
    33541 taattataat agtggctaga gagcttacag ttagcatatt aagagctata gctgctgctg
    33601 atggaaaagt aatcgcagct ggaaacagtg gaaaacttaa aacgataact caaatggtat
    33661 cgatagtgtt tttattatta ggtgctcagt ttgaaaatgg attaatatta aatataggtg
    33721 gagttcttat tcttatagct acgctactta cattgtattc tggatgggaa tatctatata
    33781 aaaataaaga actttttatg tcttctaagt aaaaaattat gctttaataa ataaaaacta
    33841 atgattaata aataaaaaat aacaacttaa aattttaaaa atgtaaaaaa gggtatacta
    33901 ttatagtata cctcttttca ttttaagaat attttctttt gtgtatataa ttattaataa
    33961 tggtttataa ataaaatgta atgaataaaa atataatcat tagtagcgtt tttaaattga
    34021 ctaggtttga tttatgttat ataattaata tataacaaga gaacaaatgt ttgtagataa
    34081 atgaagggat ggtatagaga tgagtgtaga tcaagaaaaa ttaaaagcgt tgaatgaagc
    34141 tttaggtaaa attgaaaaag attttggtaa aggttcagta atgaaattgg gagaagcaac
    34201 atctatgtct atagatgtta tatcaacagg agcgattggt ttagacatag ctattggtat
    34261 aggaggtcta cctagaggga gaatagttga agtatatggt ccagaatctt ctggtaagac
    34321 tactgttgcg cttagttgtg tagcatcagc tcaaaaagat ggaggaatag ctgcatttat
    34381 agatgcagaa catgcacttg acccagtata tgcaaaagct ttgggtgtgg atgttgacaa
    34441 cctaataata tctcaaccag atacaggtga acaggcttta gagatagcag aggcattgat
    34501 aagaagtgga gcgatagata taatagtaat agactcagta gcagcattag ttccaaaggc
    34561 tgaaatagat ggagatatgg gtgattctca tgtaggttta caagctagac ttatgtcaca
    34621 agcacttaga aagttaactg gttcaattaa aaaatcaaat tgtgttgcta tatttataaa
    34681 ccagttaaga gagaaagtag gaataatgtt tggaaaccca gaaactacta ctggaggacg
    34741 tgcactaaaa ttctattcat cagttagatt ggatgttaga aaaatagaca caataaaaca
    34801 aggtgataaa gttataggta gtagaactag agttaaagtt gttaaaaaca aagtagcacc
    34861 accatttaag caagctgaat ttgatataat gtatggagaa gggatttcaa aaattggaga
    34921 ccttttagat atagctgctg atgtagatat agttaaaaaa tcaggttcat ggtatagtta
    34981 caatgatact aaacttggac aaggaagaga aaatgttaaa aaattcttgg aggataattt
    35041 agatttaact actgaaatag atgagaaagt tagagcattt tacaatttaa atgaagaaca
    35101 tgaagaatca ggtacttcag tatcaaaaga aattgtagaa gaataagtaa aaatacaaaa
    35161 aaataactag taattaaaga catgccacgc tccctattag cttgacacaa tgttgaaaaa
    35221 attataaaat taagtgagga taaaatatct aaatttaatt ttaattatta ggaatatttt
    35281 ttaatcatag tgatgacatt ttgggattat taacgaagga ggtggatgtc atagatagtg
    35341 tagtaatgat tgtaataggt gcagctgttg gtattatagc tggatatttt gttagaaaaa
    35401 atatatctga agcaaaaatt ggacaagctg aaaacttggc caaagaaata atagataaag
    35461 cacaccacga ctcagaaaca gtacaaaaag aaaagttact agaagctaaa gaagagatcc
    35521 ataagtggag aacagaagct gaaagagaaa acagagaaag aagaacagaa gtacaaaagt
    35581 atgaaaaaag agtagtacaa aaagaagaag tcttagatag aaaattacaa aatttagaat
    35641 ctaaagaagt aaacctaagt gaaaaattaa aggtagtgga aaagaaagaa gaagaagttg
    35701 aagtaataaa aactcaacag ttagaaaaat tagaaagtat atcaggaatt acttctgata
    35761 aggccaaaga aatcatctta accaatgcgg agagagatgt aagacgtgaa atgtctataa
    35821 tgataaaaga gatagaaagt caagctaaag aagaagctga taagaaatca agagaaatta
    35881 taggatatgc aattcaaaaa tgtgcagcag atcacgtagc cgaaactact gtaacagtag
    35941 taagcttgcc taatgatgaa atgaaaggta gaataatagg tagggaaggt agaaatataa
    36001 ggactttaga aaccttaaca ggtatagacc ttataataga tgacactcca gaagccgtaa
    36061 ttttatcagg atttgatcca ataagaagag aaattgcaag aattgctcta gaaaaattaa
    36121 tagcagatgg aagaatacat ccagctagaa ttgaagaaat ggttgaaaag gcgagaaaag
    36181 aagttgacaa tattataaaa gaatatggtg aacaagctgc atttgaaact agtgtacatg
    36241 ggattcatcc ggaattaata agattattag gtagacttaa ctatagaact agttatggac
    36301 aaaatgtact taaacattct atagaagttg ctcatatagc tggtataatg gctgctgaaa
    36361 taggagcaga tattagatta gctaagaggg caggtttatt acatgatata ggtaaggctg
    36421 tagaccatga aatggaaggt acacatgttg aaataggtat ggatttactt agacgttata
    36481 aagagtctaa agaagttata catgccatga gtactcatca tggagattac gaacctcaaa
    36541 cagtagaggc tgtattagtt acagcagcag atgcaatatc agctgctaga cctggtgcga
    36601 gaagagaaac tttagaagca tatataaaga gacttgaaaa attagaagaa atagctaatt
    36661 catatgaagg tgtagaaaaa tcatttgcta tacaagctgg tagagaaata agaataatgg
    36721 taaaaccaga agctataaat gatgaagaaa tacatctatt ggctagagat atgactaaga
    36781 aaatagagga tgaacttgaa tatcctggac aaataaaagt aagcataata agagaaacaa
    36841 gagctataga atatgcgaag taaaagaaat aatatctaaa taataagaaa atatttttag
    36901 aaatttaaat ttgccacaag atatgataat atgtcttgtg gcttttaaat tgtttaagtt
    36961 taattttata taatgaaaaa ataatctagg tgctaaaagt gctacttata aaagattaat
    37021 aaaataaaga tttatatatg tattatttat ataattatcc aagtttgttt gatata
//
LOCUS       DAEPXF010000016        36860 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain HB27
            SAMN13376389-rid12479423.denovo.017, whole genome shotgun sequence.
ACCESSION   DAEPXF010000016 DAEPXF010000000
VERSION     DAEPXF010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13376389
            Sequence Read Archive: SRR10518432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 36860)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36860)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001069624.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001069624.1
            Genome Coverage       :: 315x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/15/2021 01:54:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,165
            CDSs (total)                      :: 4,076
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 89
            rRNAs                             :: 10, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 10 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 19 of 69
            Pseudo Genes (incomplete)         :: 44 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 10 of 69
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36860
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13376389-rid12479423.denovo.017"
                     /strain="HB27"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="China: Hebei"
                     /collection_date="2017"
     gene            187..432
                     /locus_tag="KRL67_000742"
     CDS             187..432
                     /locus_tag="KRL67_000742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03905 family TSCPD domain-containing
                     protein"
                     /protein_id="HBH1521647.1"
                     /translation="MKYKTTGTCATEIEFEVKENKVTNVNFIGGCDGNLKGLKVLVEG
                     MNIEDVIQKLKGIECKTKPTSCPDQLSLALENYMNIK"
     gene            467..1192
                     /locus_tag="KRL67_000743"
     CDS             467..1192
                     /locus_tag="KRL67_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HBH1521648.1"
                     /translation="MKPIIGILGNLIIMENGMFPGLERSYVNNDYINAVLKGGGSPVI
                     IPVNTDKEVIKKQIEMVDGVLISGGWDVNPQLYGEETREETTFIYPEVDEFDLIAISI
                     ALELKKPILGVCRGLQILNVSLGGTLYQDNNLIEGSYIKHTQSSKRHVATHKVDVKEG
                     SILEGILGKQLLTNSYHHQSVNQLGKGLKAIAYSKDGIIEAIEKEDENFVVGVQWHPE
                     MMVDYCNKMEKLFKYFINICSNL"
     gene            1214..2527
                     /locus_tag="KRL67_000744"
     CDS             1214..2527
                     /locus_tag="KRL67_000744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBH1521649.1"
                     /translation="MNEKNKMGLISIILLGINAVVGAGVFLLPGDAMKAFGVASIFVY
                     IFDMLLVLSMAFCFAEVAGKFNKNGAAYVYTKEAFGDFCGFEVGLMKWVIGCISWGAL
                     IVGFPTSLSAVWAPAGEPHIQKIIIVAMIVGLTIINLLGVSLSKIVQNVITVGKLIPL
                     ILFIGIGIFFIKGVNFTSSTMVPPGAGATEFGAAALLMFYSFTGFESIAVAAEDMENP
                     QKNIPIAIISVIVIASIIYILNQVVCVGILGDSLSSTSTPVADAARICFGNMGAGLVT
                     FGTLVSVGGICMCGAFVNPRSCVALADDKMLPRIFARKDKKGTPYVAIIATMLITIPI
                     ALSGSFAELAAISAVARFIQYIPTSLSVLVFRKKRPELVGTFKTPFGAVIPLIAVCVG
                     TWLLFQASMHQLIMGLGALAIGVPLYFIMKSYNRKVYGENLKKIV"
     gene            2733..3272
                     /locus_tag="KRL67_000745"
     CDS             2733..3272
                     /locus_tag="KRL67_000745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBH1521650.1"
                     /translation="MPTITLKNGVDVLIREGVREDAQSIIDFYNEVGGETHFLSFGKD
                     EYKISLEEQENAIESAKASDNSVKLIAFIDGEIVGIATIDSNQKAKGKHVGVLGIVVK
                     EKYWGIGLGKRLMLDLIEWCKSNGITKKITLVTNEENYNAIGLYKKVGFEVESILKKE
                     CYYNGVYTDLIGMSLLLGI"
     gene            3361..5346
                     /locus_tag="KRL67_000746"
     CDS             3361..5346
                     /locus_tag="KRL67_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphatase class III"
                     /protein_id="HBH1521651.1"
                     /translation="MKNLCKISDDYLNSKLKYLKLLSKQYPSISKASTEIINLEAILN
                     LPKGTEHFITDVHGEYEPFVHVLKNGSGVIKRKIEELFSNTIRDSEKKMLATLVYYPE
                     QKLDLIIKQEENIDDFYRINIYRLIELCKYASSKYTRSKVRKLLPENFKYIIEELLHE
                     HVKSEHKEEYYKSIVETIVDIGIAKEFIIAISTVIQKLVVDRLHVIGDIYDRGPRPDI
                     IVDKLIEHHCVDIQWGNHDILWMGAASGEKTCIANALRISARYANLDIVEDIYGINLL
                     PLATFAIEMYKDDPCKEFIPKVNDQSVTTTEKSLMAKMHKAISIIQFKLEGEVIRRRP
                     EFEMEHRLLLNMINYDEGTIILKGKTYKLKDTYLPTIDKKDPYKLTMEERNVIDKLVS
                     SFRGSEKLQKHVSFLFSKGSIYLKANSNLLIHGCVPLNEDGSFMSMNIMGKEYKGKAL
                     MDKMESLAREGFFFKDKAEEKLYGMDIMWYLWTGKCSSLFGKDDMTTFERYFIAEKET
                     HKENKNPYFKLRENEMACKRLFEEFDLELDESHIINGHVPVESKNGESPIKANGKILV
                     IDGGFSRAYQKTTGIAGYTLIYNSRTLQLVSHEPFNSAEEAIANESDILSTTVVVEHK
                     AKRKMVRDTDEGVKIQEEIEDLKLLLMAYKKGLIKEM"
     gene            5567..6535
                     /gene="spoIIIAA"
                     /locus_tag="KRL67_000747"
     CDS             5567..6535
                     /gene="spoIIIAA"
                     /locus_tag="KRL67_000747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage III sporulation protein AA"
                     /protein_id="HBH1521652.1"
                     /translation="MNKLSDEIINSLSTTIREKVEKISNNNLNIEEIRLRSQKPLILN
                     ANSKDYFYNQKTMTLDLNQQNSYVVTREDVEQTFQIICKYSIHSFMDDIKKGFITLRG
                     GHRVGLVGKAIVEDGQVKNIKHISSLNIRVSREIIGCSDKILSHIIKGKNQINNTLII
                     SPPQCGKTTLIRDIVRNLSNGNEDYGFKGLKVALVDERNEIAGAYLGIPQMDVGIRTD
                     IIETCPKDLGITMLLRSMSPNVIVTDEIGSEKEIKALYTALNGGIGLITTVHGDSIDD
                     IQNRKELNRLLDKELFKKVIILSAKRGAGTIEKIYDLEEKRWYFAN"
     gene            6525..7046
                     /locus_tag="KRL67_000748"
     CDS             6525..7046
                     /locus_tag="KRL67_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage III sporulation protein AB"
                     /protein_id="HBH1521653.1"
                     /translation="MQIKIIIIAFLIGSSYLIGEQIYKTYTRRHKQLNDLIRVLEILR
                     MDLSFGLYTLEEIFNRIGGNKEFCFWKFFYQISEGLHNEQSKTLEIIISENIDVLSKE
                     TYLGNKEIEELKNLILTLGKSDIESQQRMIDLSIENLKKQTYETKEDINKKGVLYKKL
                     VTFIGIGICIILI"
     gene            7063..7260
                     /gene="spoIIIAC"
                     /locus_tag="KRL67_000749"
     CDS             7063..7260
                     /gene="spoIIIAC"
                     /locus_tag="KRL67_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018589775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage III sporulation protein AC"
                     /protein_id="HBH1521654.1"
                     /translation="MEISLILKVAGVGILISVLNMILEKTDRKDWAGLTTLAGVIIVL
                     GMVITEISDLFNTVRTMFQLY"
     gene            7276..7662
                     /gene="spoIIIAD"
                     /locus_tag="KRL67_000750"
     CDS             7276..7662
                     /gene="spoIIIAD"
                     /locus_tag="KRL67_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage III sporulation protein AD"
                     /protein_id="HBH1521655.1"
                     /translation="MEIMQLIGIAIISTTLCLVIKKDRPEIANFIAIITGVIILLSVM
                     FKLNFIVDSIQDLANKANIPTMYISLIIKLIGIAYLMEFAIQLCKDCGEGNIASKLEF
                     GGKIIVMSMSFPILLSIVEMVVNIIP"
     gene            7747..8952
                     /gene="spoIIIAE"
                     /locus_tag="KRL67_000751"
     CDS             7747..8952
                     /gene="spoIIIAE"
                     /locus_tag="KRL67_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage III sporulation protein AE"
                     /protein_id="HBH1521656.1"
                     /translation="MKKRFLPMIIGFLFTFFFVNIFAIMIFANEVPSSEDKEYGETKN
                     SIDKYIDGQLDKLDINEIQDYINKEIVINDVNLKSFVKDLISGEKNILDLFNKDGLKI
                     LMFDEFKASLKVVAVILVLALLSSILKSLENSFSSGAVSQIATYIIFITMVSLTLVGF
                     KDVLQICYDAIDHTVGLMQVIMPILITFLLLIGFPITSTTLNPIFIGGVTFINVFFKN
                     FLFVSITVAFGILIINNLSKNIRLKRFFSFVKQINYVSIGAMFTVYLGLVSIQGLYVT
                     SFDKFSVKTAKFAIGNFIPVVGGFVSDSVDILLSSSQLIKNIFGGIGLILLVGICLLP
                     VIKILSVIVVYKLAAIIVEPVGEDGISNFLNEVANLMIIMLASVIAITVMFFVTVAIL
                     TSISVVSQG"
     gene            9012..9629
                     /locus_tag="KRL67_000752"
     CDS             9012..9629
                     /locus_tag="KRL67_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage III sporulation protein AF"
                     /protein_id="HBH1521657.1"
                     /translation="MLEGIKAWIVSVLIGAFIVNIVDMILPSSKIKPYVNLVLNFMFV
                     FIVITPVVGFFSKDMSLEDRILKSMGSYNKQYVDSTNALAKETGNNSLSKGYEDGLKE
                     VLKLKLDEYGYDLEDIELNGANINNIKIKEKNNSAKSNSSSNEENKNNSTKREEKNIN
                     SNDKENSKQVFKKGTEYGLNLNEEKLKNDLIKVLDVSIEDIQIDK"
     gene            9641..10195
                     /locus_tag="KRL67_000753"
     CDS             9641..10195
                     /locus_tag="KRL67_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage III sporulation protein AG"
                     /protein_id="HBH1521658.1"
                     /translation="MLKNLNDKDKRKVYSLITIAGICVVSLVLLSCFPSSKSDKQVGK
                     TETNKNAEKQVTKEQEKDDLESKLTAILSKIDGAGDVDVMVTFESSEEIQPAFNSNNT
                     TETTEEKDAQGGERTVTTSSENKTMITSNSSDPVVIKTTEPKIKGVIVVASGASDPSV
                     KETLYSAVQTSLQVAGHQVEIYSK"
     gene            10222..10911
                     /locus_tag="KRL67_000754"
     CDS             10222..10911
                     /locus_tag="KRL67_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIIAH-like family protein"
                     /protein_id="HBH1521659.1"
                     /translation="MKFNYKGRGFVIITLTAMLVVVGTVNYQLSKKSLLETSKEFKAY
                     EEAQLQKNTDDGDSSKTEDSSNVDKQDSKESADIDIVDSKASKVKEKATETSKEIKAQ
                     LSSEKNMKKASYILDMKMNREKQRNELVQDLNEMINNPSTTEESRKEASNMKLNIVKT
                     QEKELQIENLLSTKGYEEALVYISDNKVNVVVNEAKLEKKDAAKIFDLVAEQANVKYE
                     NIKLTNNNSNK"
     gene            11021..11383
                     /locus_tag="KRL67_000755"
     CDS             11021..11383
                     /locus_tag="KRL67_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HBH1521660.1"
                     /translation="MEDNKFGQVKISNDVIATIAGLAALEVEGIETTATLTDKLLKNN
                     GVKIQIEEEDVNLDVMVTIKYGMSIPDTAFKVQENVKNTVETMTGLKVSQVNIHIQGI
                     SFKKDKVDKEEAKATKKN"
     gene            11468..11974
                     /gene="nusB"
                     /locus_tag="KRL67_000756"
     CDS             11468..11974
                     /gene="nusB"
                     /locus_tag="KRL67_000756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HBH1521661.1"
                     /translation="MKKDRAQKSTTREYIMKLIYQININKEDFETLEDKVDNFLKDNS
                     EHIINRYKELALQYSKNTNLKLEDTEIEDVIDKKYINTVCKALKENHDKIDELINKHA
                     KNWTVDRMPKVDVSILRLSVCEILYLDTPNKVSINEAVELAKIYCDDKSPKFINGILG
                     SVVDEIGK"
     gene            11958..13028
                     /locus_tag="KRL67_000757"
     CDS             11958..13028
                     /locus_tag="KRL67_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-sialoglycoprotein endopeptidase"
                     /protein_id="HBH1521662.1"
                     /translation="MKLGNNIIIGIDTSCYTTSIAAISLDKKVIFNEKIMLEVKDNSK
                     GLRQSEAVFQHINNLGILSDRIKSFKDKFNVEGVCSSKKPRPVENSYMPVFNVGHNFG
                     KLLSSIYGCRFYETTHQENHIEASLLNSKLKNNNRFISVHMSGGTTEILLTSKQDSHH
                     NVCDTNLGKIAKISIKKDDKSKLYNNFGYNIDIIGGSKDISFGQLIDRVGIKLGYKFP
                     SGKYLDENALNCNLKIESGLKTSVRDGYMNLSGLENQVNKIINDNGDNTNQKEYISKL
                     VLDSVVRNMFKSLVYLCETYNVNEVVFAGGVSASKYILRELSMKLRKKHIEAYFTEPQ
                     YSTDNAVGCAIIGLNNFLGERV"
     gene            13025..14230
                     /locus_tag="KRL67_000758"
     CDS             13025..14230
                     /locus_tag="KRL67_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HBH1521663.1"
                     /translation="MKLRALDISEANSYIKRILINDPILSNLKVKGEISNFKVHSSGN
                     VYLSLKDETSKLNCVIFKSNFNRNLKLDNGVKVIANGYISVYERDGAYQLYINEIEIE
                     GIGNLHIEFNRLKEKLNKEGLFDPKYKIPIPKMPNSIGVITSPTGAVIRDIINVIKRR
                     YPKVNIKLYPVTVQGDKSAEEICEAIRFFNHMKNVDTLIVGRGGGSIEELWSFNDEMV
                     AREVFNSQIPIISAVGHETDFTICDFVSDMRAPTPSAAAEIATPSLDDINYKLGNIKS
                     RMSKSLTNQIELDQYRLETVFNKINNYLDSYTIKDKVIQLDKIYDKIIFGIENNLKLE
                     DEKLVKIGALLHNLSPLATMDRGYSITQKNGKVINSIKGLKIKDSIDIVLKDGNLECM
                     IDKIENKEG"
     gene            14232..14447
                     /gene="xseB"
                     /locus_tag="KRL67_000759"
     CDS             14232..14447
                     /gene="xseB"
                     /locus_tag="KRL67_000759"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HBH1521664.1"
                     /translation="MNLTYEEAYKRLESILSELESKNASLDESLSLYEEGISLYKHCN
                     KLLDDAKLKISKFNQLGIEEDFKIEEE"
     gene            14450..15337
                     /locus_tag="KRL67_000760"
     CDS             14450..15337
                     /locus_tag="KRL67_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HBH1521665.1"
                     /translation="MEFKQCLKEKASFVEKVLKEYMPKEEGYQKTVIEAMNYSLSAGG
                     KRLRPILTLEACKIVGGNEDEAIPFAIAIEMIHTYSLIHDDLPALDNDDLRRGRPTNH
                     KVYGEAMGILAGDALLNYAFEVMLAGSINKENPEKYLKAINEIAKGAGIYGMIGGQVV
                     DVESENKQIEKEKLDYIHMNKTAAMMVGCMRAGATIGGANFEQMEEITKYAKNIGLSF
                     QIVDDILDIVGDEAKLGKKVGSDIENHKSTYPSLLGLDKSKEIAHNLIDEAKKSIEKL
                     SDDVDFLKGLAEYIIDREY"
     gene            15367..15804
                     /locus_tag="KRL67_000761"
     CDS             15367..15804
                     /locus_tag="KRL67_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divergent PAP2 family protein"
                     /protein_id="HBH1521666.1"
                     /translation="MDFFSEIFNNGALGISLIACFLAQFIKIFTGKEKRIELSRILIS
                     GGMPSSHSSFVTSLATVVGIEKGFNSTDFAIITVLALIIMYDAAGVRRAVGKQATILN
                     QMVADIQHGKHIEQKKLKELIGHTPLEVWFGALLGIVTALILM"
     gene            15838..17703
                     /locus_tag="KRL67_000762"
     CDS             15838..17703
                     /locus_tag="KRL67_000762"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HBH1521667.1"
                     /translation="MYKYLDKVNSPKDIKNMSIEEMDLLAKDIRKFLVKSVSKTGGHL
                     ASNLGVVELTLALHKVFDSPKDKIVWDVGHQSYVHKIVTGRKDCFVSLRQFNGLSGFP
                     KENESPHDIFDTGHSSTSISIATGIACARDIKKENYSVISVIGDGSITGGMALEALNQ
                     LGYIDTNMIVILNDNEMSIDKNVGGMSKYLSSIIRNSTVEKMTDEVDKILNVTQTGEI
                     LSKTAHRFKDKLMYSFSPQDCSFFDSLGIRYYGPIDGHNTKELIDILRKAKHKKGPVL
                     LHVITKKGKGYRFAEEQPDKYHGVSKFDIKTGVTSAKVKSMSISVGEKLVDMANNNED
                     IVAITAAMPSGTGLNLFESAYPKRYYDVGIAEQHATGFAAGLAKNGMKPYFAVYSSFL
                     QRAYDQVIHDVCITKKPVTFLIDRAGLVGNDGETHHGMFDLSYLNSIPNIVVMAPKDT
                     REMELMMDLSLKLDCPLAIRYPRGSSYYLDKGEYGEIVLGKYEVLDDGQDTVILCIGS
                     MVKHALEAKEILSREGINPTIVNARFLKPIDEDMLKVLLKNHKNVVTIEDNIVTGGFG
                     SRINKFIIDNEYNVNILNIAIPEEFVKHGNADELYDFVGLSPKSIADKIRKLVIE"
     gene            17836..18648
                     /locus_tag="KRL67_000763"
     CDS             17836..18648
                     /locus_tag="KRL67_000763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HBH1521668.1"
                     /translation="MKKRIDLLLVEQGYFESRERAKKAIMAGLVFVDNQRCDKAGTEV
                     KEDCSIEVKGNPIPYVSRGGLKLEKAMKNFDLTIDGKVCMDIGASTGGFTDCMLKNGA
                     IKVFSIDVGYGQLAWKLRQDDRVVCMERTNIRNVTIEDTKQFADFASIDVSFISLKLV
                     LPKAKELVRHDGEIVALIKPQFEAGREKVGKKGVVREKSTHIEVIKMISDFSVENGFE
                     ILGLDFSPIKGPEGNIEYLIHLRNGNEGYEFDGEIYNNKIVEVVEASHNLDK"
     gene            18668..20347
                     /gene="recN"
                     /locus_tag="KRL67_000764"
     CDS             18668..20347
                     /gene="recN"
                     /locus_tag="KRL67_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HBH1521669.1"
                     /translation="MILELYMKNCALVEELRLNIDKNLNILTGETGSGKSIIIDALGL
                     CLGDKYDRSFLRKGTDKGLVEAVFFSDNIYLKKILEENDISMEDDNLLVITRLIYSDG
                     KSTARVNGRTVKVSLLKEIASTLIDIHGQHQNQALFNKDTHLKFLDLFGENELEGFKI
                     AYKNVYHKYSEVKKALNCLTENKDEMQIQREIDLLRFQINEIEAANLNKNEYEDLLKQ
                     REVYRNSEKIYNNLNSSYSKLHNGEYNVIDLIGLASKELNDISKYDSVLSEYSDTVER
                     IMYELQDISGEIRNYKDNIDFEPYELEQIELRIDEINNLRRKYGDSIDDIFEYYGKIK
                     DRLDEILNRDERVEQLKSKLMNIEEDLKIKASKLTKARIEVATTLEKILLDELKSLNM
                     KNVMFKVNFEKGLFTLDGVDDIEFMISFNLGEDIKPIYKVASGGEMSRFMLAFKTILA
                     DIDDIDTLVFDEIDTGISGIAAQIVGEKLSDIAKKKQIICITHLPQIAANADTHYCIE
                     KDTSNNRTFTNVSKLNESQRKNEIARLIAGNNITEKTIEHASEIIELAKKC"
     gene            20563..21429
                     /locus_tag="KRL67_000765"
     CDS             20563..21429
                     /locus_tag="KRL67_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH1521670.1"
                     /translation="MNKKDTMHLIFIIVIFILLWYIVVAVSEINKNIESCSNKLDYLS
                     QEIMSTKSDIGNTINEEMSKSYITKSIDLKVKKIEKKECIIDIDVQLSKLGKNGKVFF
                     MYKEDSDKWNETEMTKHGELSYACEIKINTGSEYDYKVVTSGAISESSDVQKLTKSDY
                     MPEQPIFNSGIRDNREYFIEIVENSNLFNHESEKSKNKAYDNIRLEKVDIIVNEGKKD
                     TIYKAKLAEGLDDGKTNNSNRNQVNYEVSFPKRDIDDIIYIKAKLMYNNGVEYTKDIT
                     EDITMGLQDLEK"
     gene            complement(21522..22013)
                     /locus_tag="KRL67_000766"
     CDS             complement(21522..22013)
                     /locus_tag="KRL67_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBH1521671.1"
                     /translation="MADKMAQNITLQFVEEKDLENLENVKLLFTEYSNSLNIDLCFQD
                     FNNELKTLPGKYKKPSGSLILAFVDENLAGCVALKKLEYKICELKRLYVRNQFRGLKI
                     GKILLEEIIEEAKKIGYTHMRLDTLPSMKSAQGLYEKFGFYDIEPYTYNPIEGARYME
                     LKL"
     gene            complement(22074..22514)
                     /locus_tag="KRL67_000767"
     CDS             complement(22074..22514)
                     /locus_tag="KRL67_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBH1521672.1"
                     /translation="MKLNVRIADINNWHDLVSLSVDKSQLDYIESNALSIAESKFITA
                     WIPVGIYDENSLIGFAMYGRLEDDRVWLDRFMIDSKYQGKGYGKASLDFLVNHLKNEY
                     NCDELYLSIFEDNKMAIKLYKDFGFEFNGELDYGGEKVMVLKSN"
     gene            22675..23733
                     /locus_tag="KRL67_000768"
     CDS             22675..23733
                     /locus_tag="KRL67_000768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase S55"
                     /protein_id="HBH1521673.1"
                     /translation="MNKINKNFKNIWIILGAVSIFTMVIIFNKNCNVNKETQIEAFTI
                     KNHDKKFVYPMGNIIGVKANTDGVLVLGYEEEDIDYIGGIQIGDNIVKIDNKRIKNSQ
                     DVSEILNKIKKNKVEVTFERKNKYKTEVIETKKENGKYKLGLWVRDKISGIGTMTFYD
                     PSMEKFKAIGHAIKDSDTNELLKIKQGYIYKPEQLKIVKASNEKVGKIKGDFNDSNLM
                     GNFSNNSELGISGNIIENHNKEFNVANKEKQLIEVGRPQDVKIGDAVILFEDKNKNIT
                     SYDIKIESIVYDKGNYRDMVIKVVDDKLLEYTGGIVQGMSGAPIIQNNKIIGAITHVF
                     RDNPKKGYGIFIDEMIKL"
     gene            23875..24699
                     /gene="spo0A"
                     /locus_tag="KRL67_000769"
     CDS             23875..24699
                     /gene="spo0A"
                     /locus_tag="KRL67_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation transcription factor Spo0A"
                     /protein_id="HBH1521674.1"
                     /translation="MGGFLVEKIKIVLADDNKDFCQVLKEYLSNEDDIDILGIAKDGI
                     EALDLVKKTQPDLLILDVIMPHLDGLGVIEKLNTMDIPKMPKIIVLSAVGQDKITQSA
                     INLGADYYIVKPFDFVVFINRIRELVSNRVTQVEPKPRPVQETQMTRSDFVKNVGNIE
                     TEITNIIHEIGVPAHIKGYLYLREAIKMVIDNVELLGAVTKELYPSIAKKFNTTPSRV
                     ERAIRHAIEVAWSRGKVDTINQLFGYTVHNTKGKPTNSEFIAMIADKLRLEHSMVK"
     gene            25112..26233
                     /locus_tag="KRL67_000770"
     CDS             25112..26233
                     /locus_tag="KRL67_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH1521675.1"
                     /translation="MRVEAPIKVDRKTKKLAKRVESGEIAVINHIDIDEVAANSLVEA
                     KIKLVINAAPSISGRYPNKGPGILTENNILIIDNVGEELFEELKEGETIEVVDGKIYR
                     KGKFLGAGEVLDKYEVSHQIKAAYENLAVELDRFIDNTIDYAKKEKGFILGEVEIPKV
                     KTNYANKHVLIVVRGQDYKEDLSTMLSYIEEVKPVLVGVDGGADALIEFGYTPDVIVG
                     DMDSVSDEALKKASEIVVHAYTDGRAPGLKRVEELGLDAVVFPAPGTSEDIAMLIAYE
                     YKAELIVAVGTHSNMIDFLEKGRKGMASTFLVRLKIGSKLIDAKGVNLLYRSKLKIKY
                     IWALIATALFPVLVVASLSPGVQQFIQLMQLKFRVLLQM"
     gene            26251..27126
                     /locus_tag="KRL67_000771"
     CDS             26251..27126
                     /locus_tag="KRL67_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper transporter"
                     /protein_id="HBH1521676.1"
                     /translation="MHINMKYYIVTIGAIFIALGIGMLVGFNLNNNQQLSEQQANIIN
                     DLDDKFNILKEKNDKLDSDLASVNRDYEEAVNFINKNVDKILAGSLNGKNIGIISANE
                     NDDYTKNIEDIINKSNGSIAFNIVLKENITNPEKLKEMSTKLGTDVKNANDAVNYIID
                     TLKKEDASDILTYLQELDVIKFNFIGDTYLKYDSVVIAGGNDAKDNTKQFEKIEKFVV
                     SKLKSENKYLVEVQNTGVKTSYVELYSKNKVATIDNIDEGIGSISLAILLQQGNIVGN
                     FGRLDTATSLLPSIK"
     gene            27150..27842
                     /locus_tag="KRL67_000772"
     CDS             27150..27842
                     /locus_tag="KRL67_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HBH1521677.1"
                     /translation="MDSYISIIIPAYNEESRIKYTLESILDIKQINEIIVVDDGSIDN
                     TAKVVSKLENEKIKFFKLDKNRGKGYALNYGIKLAMKNADIIGFLDGDLGSSAKEVEK
                     LIVPILNGEADVTIAKFPPAKRKGGLGFVKGLAKNSVFEMTGVELDATLSGQRIFKRE
                     VLEKFDEIPFGYGVEVGMTIDILKYGFKIKEVLVNMTHNETGRNLKGFIHRGKQYYHI
                     KKVLGQKKKEWR"
     gene            27850..28674
                     /locus_tag="KRL67_000773"
     CDS             27850..28674
                     /locus_tag="KRL67_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HBH1521678.1"
                     /translation="MKDYILYTILLLTGLFGTYAVIPLFKNLLINGNVLRPNYKKDMI
                     PVSMGIVFLPMIVINGIILAFFTTEFKDLAYIFMFMFGMISMFFAGILDDVIGNRDVS
                     GLKGHFKSLFKGSLTTGGFKALFGGFVGLIVSVAISKDIIDIIVNTLIIALSTNLMNL
                     LDLRPGRAIKAYLFIMVIIFLTLTGFVQVLPLLIVPNVLAYFNYDLKARAMMGDTGSN
                     VLGISIGMLISFGYTFNIRLGWLIFVVIMHIFTEKYSLTKIIEKNKLLNFIDKLGR"
     gene            28679..29746
                     /locus_tag="KRL67_000774"
     CDS             28679..29746
                     /locus_tag="KRL67_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3866 family protein"
                     /protein_id="HBH1521679.1"
                     /translation="MISKRVGIVESIVSQTETLDDIRVNINGEIQRAYNYPKISGTIN
                     IGDEVVLNTTAVELSLGTGGYHFVITNLNNIESTLTEGGHIMKLRYTPLQIKVDSVEE
                     QESIYHDKFANFKGLEGLSVVVGTLHSMLTPFAASFKRNNPNKKLVYIMTDGASLPIY
                     LSKNVDILKEKKLIDSTITIGNAFGGDYECINIYTALITAKEILKADAVFVSMGPGIA
                     GTGTKYGFTGIEQGSILDAVKKLEGRAIAIPRISFADKRERHQGISHHSMTVFKEIVN
                     VNVDIPITIYDDEKLNFIKEQINLNGLDKKHNIIYIDNNKTKEDLDYFNLKVKSMGRN
                     YEQDEAFFKAASTAAYYLMEV"
     gene            29748..30284
                     /locus_tag="KRL67_000775"
     CDS             29748..30284
                     /locus_tag="KRL67_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HBH1521680.1"
                     /translation="MVLEEKTISSDRVYTGKVITLKVDTVEIPGQGYQKRELVEVGGA
                     VGIVAITDDNKVVLVKQFRKPIEKPIFEIPAGKLEKNESPKECAERELKEETGYSAKN
                     IKLIHKFFTSAGFSNEIMFVYLATGLTPGENNLDADEFLDVYEIELEEAYNMVLKNDV
                     EDAKTSIGLLLVKDMFKN"
     gene            30352..30957
                     /locus_tag="KRL67_000776"
     CDS             30352..30957
                     /locus_tag="KRL67_000776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein M"
                     /protein_id="HBH1521681.1"
                     /translation="MRRTYRKNDFRELNRQIIIIGLLFMMSIVVGSYINKILPGSSNN
                     ILNNINPAVEYYNLNISIKDTVIQNLKSDAVFMGSIALLSLFVVTIPAVLVAFVLKGM
                     SIGYTINSCILALKFKSIKMILIILFKNLIIIPCAIILALISLSYFKEMVYEFKKKNR
                     KNMQFLIKRYILNIIIIIALSLGLQLILNTASIGIIKFLAK"
     gene            30969..31883
                     /locus_tag="KRL67_000777"
     CDS             30969..31883
                     /locus_tag="KRL67_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HBH1521682.1"
                     /translation="MDIIEGYIDYIKDKKKLSENTVASYFMDIKKYMEYINQKSIKLV
                     DIVENDIISYLIKLEKDNVSIATIARMISSIKSFHDYLFLNHICTNNPAKDIKKPKIK
                     KENINILTEEEIERLLNFPKLTTPKLIRDKAIFEVLYGTGIKVSELVEMNIEDIDLDI
                     DYIYCNSCCKNQRVIPLCDITKLYLEKYLKEARPKMAVEGEKSLFVSSLGQRFTRQGL
                     WKVIKKYSNLANIDKNINPTMLRHSFAIHLLNEGANIAVVSKILGNVNLSSLQVYLNH
                     IDKNVRREIKEKHPRNDVDVELQKAEAK"
     gene            31925..33097
                     /locus_tag="KRL67_000778"
     CDS             31925..33097
                     /locus_tag="KRL67_000778"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HBH1521683.1"
                     /translation="MSRVIWIVIDSVGIGALPDAENFGDSKDVSTLGNIFKKHPDIQI
                     PNMRKLGIGNIDGVDFFESIKEPIGCFGKCKEMSQGKDTTTGHWEMTGIIVDKPFKTF
                     EDGFSKEIIEEFEKKTGRKVVGNKPASGTVIIDEYGEHQIETGDVIVYTSADSVFQIA
                     ANEEVIPLEELYNMCKIAREIMMGDNAVARVIARPFIGKKKGEFVRTSNRRDYSLDPF
                     EPTVLDNIKESGLDVLAVGKIEDIFNGKGITDAIHTKSNMDGVDETLNYMKQDNKGLI
                     YSNLVDFDSKYGHRRDPEGYKKALEEFDSRLPEIMANMRKDDILIINADHGNDPTYKG
                     TDHTREYIPVMIYGNKIKKGFNLGVKDTFADIGATVADILNVKLPKHGSSFKEDLF"
     gene            33144..33956
                     /locus_tag="KRL67_000779"
     CDS             33144..33956
                     /locus_tag="KRL67_000779"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HBH1521684.1"
                     /translation="MFEKIAQSSEFINSKINVKPKIGLILGSGLGDLANDIEDSVTIK
                     YSEIPNFPVSTVAGHAGQLVIGKLEGKEVIAMQGRFHYYEGYSQKEATFPVRVMKELG
                     VEILIVTNAAGGVNKEFKAGDLMLIRDHINFSGSNPLVGKNDDRFGARFPDMSDAYSS
                     KYVDVVKNCAKECNIDVKEGVYMFFSGPNYETPAEVRMAQILGADAVGMSTVPEVIVA
                     SHSNIGVIGISCITNMAAGILNQPLSHEEVIETTQKVKSEFMNLVKTTVKNL"
     gene            34160..35485
                     /locus_tag="KRL67_000780"
     CDS             34160..35485
                     /locus_tag="KRL67_000780"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-nucleoside phosphorylase"
                     /protein_id="HBH1521685.1"
                     /translation="MRMYDIIKKKRDNLELSKKEINYFIENYSKGDIPDYQASALLMS
                     IYLNKMNKQETVYLTEAMMNSGDMINLSEIDGIKVDKHSTGGVGDKTTIALIPLVASC
                     GAPVAKMSGRGLGHTGGTIDKLESIPGFSAEMEISKFIDSVNKSKIAVCGQSAKVAIA
                     DKKIYALRDVTATVDNISLIASSVMSKKLASGADAIVLDVKTGDGAFMKTLEESFELA
                     KSMVDIGTGMNRDTIGIVTDMDEPLGFAVGNSLEVIEAIETLKGNGPKDLVELCEVLG
                     AYMLVLSKVAHDFEDGVEKIRESIKSGTAIQKLKEFIENQGGNKDVVDDYSLFKKSKY
                     KYPVKSTKSGYISKIKAEDVGVSAMILGAGRETKDDILDLSAGIILEKKVGDYVNEGE
                     VLAYMYYNDEQKLSLAKRRFIDSYSIVDSKIEKNKLIYGIVTKEDIKKF"
     gene            35698..36777
                     /gene="mltG"
                     /locus_tag="KRL67_000781"
     CDS             35698..36777
                     /gene="mltG"
                     /locus_tag="KRL67_000781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HBH1521686.1"
                     /translation="MNFKENRLKIAVLIIVILIILAGIFVFIQIGPYDKNNKKDVIID
                     VPSGASVGKISDILYENKLIKNELLFKLLVKVSNKAPSIKSGTYLLNQSYSNNDIISL
                     LVSGKIYQDGIKVTIPEGATSKEIIAMLVSKNLGDKATFENLIKKPQEFYDKFPYLKE
                     DGITSLEGFLYPETYYFNSKKQSEEDILSEMLKVFDSKYTDKFKKKQKELNMTLQEVM
                     EMASIIEKEAVLDKDRPIIASVFYNRLKVGMPLQSDATIQYIFEERKKIVTYDDLKID
                     SPYNSYKNKGLPPTPISNPGIESIEAALYPDKTDYLYFVAKIDGGNNYSTNYQDHLKY
                     VKEYKEARDKQSKDTKATNKENTKR"
BASE COUNT        14972 a         3660 c         6745 g        11483 t
ORIGIN      
        1 aagataaaat aacccattac ctatttttat ttgtaagaaa agtgtatata gaattaaaag
       61 taaaaaatac aactaataaa actattgaaa acactagata aataataaat aaaaataatt
      121 ataaattttg gcatatatat tgctaatcat agagataaga cgacaaaata attgggaggt
      181 ttagtgatga aatacaaaac aacagggact tgtgctacag aaattgaatt tgaagtaaaa
      241 gaaaataaag ttacaaatgt aaactttata ggtggatgtg atggaaattt aaaagggcta
      301 aaagtattag ttgaaggtat gaatatagaa gatgtaatac aaaagttaaa gggaatagag
      361 tgtaaaacaa agccaacatc ttgtccagac caactgtctc tagcattgga aaattatatg
      421 aatataaaat aatacataaa acaatcgatt agttaggagc ttaattatga aaccaataat
      481 agggatttta ggaaatttaa taataatgga aaatggtatg tttccaggtc ttgaaaggtc
      541 atatgtaaat aatgattata taaatgcagt actaaaaggt ggaggtagtc cagtaataat
      601 tcctgtaaat acagataaag aagttataaa aaaacagatt gaaatggttg atggagtctt
      661 aatatctgga ggatgggatg tcaatcctca actgtatgga gaagaaacaa gagaagaaac
      721 gacatttata tatccagaag tggatgaatt tgatttgatt gcaataagta tagcattaga
      781 gcttaaaaag cctatattag gtgtatgtag gggactgcaa attttaaatg taagtttagg
      841 aggaacttta tatcaagata ataatctgat agaaggaagt tatataaaac atacacaatc
      901 ttctaaaaga catgtagcta ctcataaagt tgatgtcaaa gaaggaagta ttttagaggg
      961 aattttagga aaacaattac ttacaaacag ttatcatcat caaagtgtca atcagcttgg
     1021 aaaaggattg aaagccatag catactcaaa agatggtata atagaagcta ttgagaaaga
     1081 ggatgaaaat tttgtagtag gagtacagtg gcatccagaa atgatggtag attactgtaa
     1141 taaaatggaa aaactattta aatattttat aaatatttgt tcaaatttat aatatacctt
     1201 ggggggattg aacatgaacg aaaagaacaa aatgggattg ataagtatta tacttttagg
     1261 tattaatgca gtagtaggag ctggagtttt tctattacca ggagatgcaa tgaaagcatt
     1321 tggtgtagca agtatatttg tatatatatt tgatatgttg ttagttttat ctatggcatt
     1381 ctgttttgct gaagttgcag gtaaattcaa taaaaatggt gctgcctatg tatatactaa
     1441 agaagctttt ggagattttt gtggttttga agtaggtctt atgaaatggg taataggatg
     1501 tatatcttgg ggagctttga tagtaggttt tcctacatca ttatcagcag tatgggctcc
     1561 agcaggtgaa ccacatatac aaaaaattat aattgtagcc atgatagttg ggcttacaat
     1621 tataaactta ttgggtgtat ctctatcaaa aattgtacaa aatgttataa ctgtaggtaa
     1681 attaattcca ttaatactat ttataggaat aggtatattt tttataaaag gagttaactt
     1741 tacttcttca acaatggtac ctccaggagc tggagcaaca gagtttggag cagcagcact
     1801 tttaatgttt tattcattta caggatttga atctatagca gttgcagcag aagatatgga
     1861 aaatccacaa aaaaatatac caatagcaat tataagtgta atagtaattg catcaataat
     1921 atatatatta aatcaagttg tatgtgttgg tatactagga gattcgttat ctagtacatc
     1981 tacaccagtg gcagatgctg caagaatatg ctttggaaat atgggagctg gattggttac
     2041 ctttggaaca ttggtttctg ttggtggaat ttgtatgtgc ggtgcttttg taaatccaag
     2101 aagctgtgta gctttagctg atgataaaat gttaccgaga atatttgcaa gaaaagataa
     2161 aaaaggtaca ccatatgtag cgataatagc tactatgtta atcactatac caatagctct
     2221 atctggaagt tttgctgaat tagcagcaat aagtgcagtt gctagattta tacaatacat
     2281 accaacatct ttatccgtat tagtgtttag aaagaaaaga ccagaattgg taggtacttt
     2341 taaaactcct tttggagcag tgattcctct aattgctgta tgtgttggaa catggttatt
     2401 attccaagca tctatgcatc aattaattat gggattagga gctttggcaa ttggagtacc
     2461 attatacttt ataatgaagt cttataatag aaaagtttat ggtgaaaatt taaagaaaat
     2521 agtttaatta tgttataaca atttattgat ttaaaattag atattggcag aaaattaaat
     2581 acaaataagt atctataagg ctatattaaa atattattaa ttttttattt taatatagcc
     2641 ttctattgtt atcaataaat gaaaaatatt gtaacagtag aagaacaata gtattatgtt
     2701 tatagacaaa aaacttatta ggaggtaaat ttatgccaac aataacatta aaaaatggtg
     2761 tagacgtact tattagagaa ggagtaagag aagatgctca aagcattata gatttttata
     2821 atgaagtggg tggagaaact cattttctgt catttggaaa ggatgaatat aaaatatctt
     2881 tggaggaaca agaaaatgct atagaatcag caaaagcatc tgataactcg gttaaactga
     2941 ttgcatttat agatggtgaa atagttggga ttgcaacgat tgattctaat caaaaagcaa
     3001 aaggaaagca tgtcggagta ttaggaattg ttgtaaaaga aaaatattgg ggaattggat
     3061 tgggtaaaag gcttatgtta gaccttatag aatggtgtaa atcaaatgga ataaccaaga
     3121 agataactct tgtcactaat gaagaaaatt ataatgctat aggattatac aaaaaagttg
     3181 gatttgaagt agaaagtata ctaaagaaag aatgttatta taatggtgtt tacactgatt
     3241 taatagggat gtcactttta ttaggaatat aattagtgta cattttatct cgaaaaaagt
     3301 atattaagta tggtataatt aaaaaagaat ataacttaat ataattttgg aggacgaatt
     3361 atgaaaaatc tgtgtaaaat atcagatgat tatttaaaca gcaagcttaa gtatttaaag
     3421 ttattatcaa aacaatatcc aagtatatca aaagcaagta cagaaataat aaatttagag
     3481 gctatattaa atcttccaaa gggaacagag cattttataa cggatgtaca tggtgaatat
     3541 gaaccatttg tacatgtatt aaaaaatggt tctggcgtaa taaagagaaa aatagaagaa
     3601 ttgttttcaa atacaataag agacagcgaa aaaaagatgt tagcaactct tgtatattat
     3661 cctgaacaaa agttagacct aataattaag caagaggaaa atatagatga tttttataga
     3721 ataaatattt ataggcttat agaactttgt aaatatgcgt ctagcaaata tactaggtct
     3781 aaagttagaa aactgttacc agaaaatttt aaatacataa tagaagaact tttgcatgag
     3841 catgttaaaa gtgaacataa agaagagtat tataaaagta tagttgaaac aatagttgac
     3901 ataggaatag ctaaggagtt tataattgct atttctacag ttatacaaaa attggttgta
     3961 gatagacttc atgtaatagg agatatttat gatagaggtc caagaccaga tataattgtc
     4021 gataagctta ttgaacatca ttgtgtagat attcaatggg gaaatcatga tatattgtgg
     4081 atgggtgcag cctcaggtga aaaaacttgt atagcaaatg cacttaggat atcagctaga
     4141 tatgcgaatt tagacattgt agaagacata tatggaataa atctattgcc tttagccact
     4201 tttgctatag aaatgtataa agatgaccct tgtaaagaat ttattccaaa ggttaatgac
     4261 caaagtgtaa caacaacaga aaagtcttta atggcaaaaa tgcacaaagc aataagtata
     4321 attcaattta agcttgaagg tgaggttata agacgaagac ctgaatttga aatggaacat
     4381 agacttttgc ttaatatgat aaactatgat gaaggtacga ttattttaaa aggtaaaaca
     4441 tataaattaa aagatactta tttacctaca atagacaaaa aagacccata taaattaact
     4501 atggaagaaa gaaatgttat tgataaatta gtatcttcat ttagaggtag tgaaaaatta
     4561 caaaaacatg tttcattttt gttttctaaa ggaagtatat atttaaaggc taactcaaat
     4621 ttacttattc atggatgtgt accattaaat gaagatggaa gctttatgtc tatgaatata
     4681 atgggaaaag agtataaagg taaggctctt atggataaga tggagtctct agcaagagaa
     4741 ggatttttct ttaaagataa ggcagaagaa aaactgtatg gcatggatat tatgtggtat
     4801 ttatggactg ggaaatgttc atcattattt ggtaaagatg atatgactac atttgaaaga
     4861 tactttatag cagaaaaaga gactcataaa gaaaataaga atccatattt taaacttaga
     4921 gaaaatgaga tggcttgtaa aagattattt gaagaatttg atttagaact tgatgagtct
     4981 catataataa atggtcatgt tcctgtggag agtaaaaatg gagagagccc aataaaagct
     5041 aatggtaaaa ttctagttat agatggtggt ttttctaggg cttaccaaaa aactactggt
     5101 atagctggat atactttgat atacaattct agaactttgc aattagtatc gcatgaacca
     5161 tttaattctg ctgaagaagc cattgcaaat gaaagcgata tcttatcgac aactgttgta
     5221 gtagagcata aggcaaagag aaaaatggta agagatactg atgaaggagt aaagatacaa
     5281 gaggaaatag aagatttaaa actactttta atggcttata agaaagggtt aataaaagaa
     5341 atgtagatat tatagaggca cttcataatt gaagtgtctt tttgttaata cttaaatata
     5401 ttataaagat taataatagt agtttatatt atacttacta ggtgaatcca agataaatgg
     5461 gttcactttt tttatttata aacattatta aaaaaatata gttgttttgg ttatattcct
     5521 tgtcctttat aaatagaatt atataaacaa aggaggagga caagagatga ataaactttc
     5581 tgatgaaata ataaactctc tttctacaac tataagagaa aaggtagaaa aaatatcaaa
     5641 caataatcta aacatagaag aaattaggtt acgctcacaa aaaccattga tactaaatgc
     5701 aaactctaaa gattattttt acaatcaaaa aactatgaca cttgatttaa atcaacaaaa
     5761 ctcatatgta gtaacaagag aagatgtaga gcaaactttt caaataatat gtaaatattc
     5821 aatacattca tttatggatg acataaaaaa aggattcata actttgagag gaggtcatag
     5881 agtagggtta gttggaaaag caatagtaga agatggacaa gtcaaaaata taaagcatat
     5941 atcatcttta aatataaggg tatcaaggga aattattgga tgctcggata agattttaag
     6001 tcatattata aagggaaaaa atcagattaa taatactttg ataatatcac cacctcaatg
     6061 tggaaaaaca actttaataa gagatatagt aagaaattta agtaatggca atgaagatta
     6121 tggatttaaa ggtttaaaag tagctttggt agatgagcgt aatgagatag caggagctta
     6181 tctaggtatt ccacaaatgg atgtgggaat aaggactgat ataatagaaa cttgcccaaa
     6241 agacttgggt attacgatgc ttttaaggtc tatgtcacca aatgttattg tgacagatga
     6301 aattggaagt gaaaaagaaa taaaggcatt atacacagca ttaaatggag gtataggatt
     6361 aataactact gtgcatggag attcaataga tgatatacaa aatagaaaag aattaaatag
     6421 acttctggat aaagagttat ttaaaaaggt tataattctt tctgcaaaaa gaggagcagg
     6481 aacgattgaa aaaatttatg atttagaaga aaagagatgg tattttgcaa attaaaataa
     6541 ttattatagc tttcttgata ggaagtagtt atttgatagg tgaacaaata tacaaaactt
     6601 atactcgtag acataaacaa ttaaacgatt taataagagt tttagaaatt ttgaggatgg
     6661 atttatcttt tggactttat acattagaag aaatctttaa tagaataggt ggaaataaag
     6721 agttttgttt ttggaaattt ttctatcaaa tatcagaagg acttcataat gaacaaagta
     6781 aaactttgga aataataata tcagaaaata ttgatgtatt aagtaaagaa acttatctag
     6841 gcaataaaga aattgaggaa ctgaaaaatc taatacttac tttagggaaa agcgatatag
     6901 agtcacaaca aagaatgata gatttatcta tagaaaattt gaaaaaacaa acctatgaga
     6961 caaaggaaga tataaacaaa aaaggcgtat tgtataaaaa attagtaaca ttcataggta
     7021 taggtatatg tattatattg atttaatatg gggaggttta ttatggaaat atcattgata
     7081 ttaaaggttg caggtgtcgg tattctaata tcagtattga atatgatatt ggaaaaaaca
     7141 gatagaaaag attgggcagg tcttactaca ctagcaggtg taataatcgt attgggtatg
     7201 gtaataacag aaataagtga cttgtttaat actgtaagaa caatgtttca actttactaa
     7261 agaggaggga gtaatttgga aataatgcaa ttaataggaa ttgcaatcat ttcaactaca
     7321 ctctgtttag taattaaaaa agatagacca gagatagcaa atttcatagc aataataact
     7381 ggagtaataa tactattgtc agttatgttt aagctaaatt ttatagtaga tagtatacaa
     7441 gatttggcta acaaggctaa catcccgact atgtatatat cattaataat aaaattaatt
     7501 gggatagcat acttaatgga atttgcgata caactttgta aagattgtgg ggaaggaaac
     7561 attgcttcaa aactagaatt tggaggtaaa ataatagtta tgtcaatgtc tttccctata
     7621 cttttgtcca ttgtagaaat ggtggtgaat ataattccat agatgtgtat cttggaatga
     7681 ctataagtat attagagagc tttcgttgta ttgaggttta tagagtgaag agaggcatgg
     7741 ttgcttatga aaaaaagatt tttaccaatg attataggtt ttttatttac tttctttttt
     7801 gtaaatatct ttgctattat gatatttgct aatgaagtac caagtagtga agataaagaa
     7861 tatggtgaaa caaaaaatag catagataag tatatagatg gtcaattaga caagctagat
     7921 ataaatgaga ttcaagacta tataaataaa gaaatagtta ttaatgacgt taatttaaaa
     7981 tcatttgtaa aagatttaat tagtggagaa aaaaatatct tagatttatt caataaagat
     8041 ggattgaaga tattgatgtt tgatgaattt aaagcaagtt taaaggtagt agcagtaatt
     8101 ttggtactgg ctctgttatc atctatttta aagagtctgg aaaactcttt ttcatcaggt
     8161 gcagtaagtc aaatagcaac atatataata tttatcacaa tggtatcact tacacttgtt
     8221 ggttttaaag atgtattaca gatttgctat gatgcaatag accatactgt aggtcttatg
     8281 caagttataa tgccaatatt aataacattt ttacttttaa ttggattccc aataacatca
     8341 actactttga atcctatctt cataggagga gtaacattca taaatgtatt ttttaaaaat
     8401 tttctatttg tatctattac tgttgcattt gggattttaa taataaacaa tttatcaaaa
     8461 aatataaggc taaagagatt tttctccttt gtaaaacaaa taaattatgt ttctattgga
     8521 gctatgttta ctgtttattt aggtcttgta tccatacaag gattgtatgt aaccagtttt
     8581 gataaattca gtgtaaagac tgctaaattt gctataggta attttattcc tgttgtaggt
     8641 ggatttgtat ctgactctgt ggatatttta ttatcttcat ctcagcttat aaaaaatata
     8701 tttggaggaa tagggcttat cttacttgtt ggaatttgtt tacttccagt aataaaaatt
     8761 ttgtcagtaa tagtagtata caaattagca gcaataattg tagagcctgt tggagaggat
     8821 ggaatatcta attttttaaa tgaagtggca aatttaatga taataatgtt agcatcagtg
     8881 attgctataa cagtaatgtt ctttgtaact gtagctatct tgacctcgat aagtgtcgta
     8941 agtcaagggt aatttatata tcagtgaaaa ataattacat ttaatctaat gtttttagag
     9001 aggaggaagc tatgttagag ggcataaagg catggatagt tagtgttttg ataggagcat
     9061 ttatagtaaa tatagtagat atgattttac caagttctaa gataaaacct tatgtcaacc
     9121 tagttttaaa ctttatgttt gtatttattg taataactcc agtagtagga tttttttcaa
     9181 aagatatgag tttagaagat agaatattaa aatctatggg aagttacaat aaacagtatg
     9241 tagatagtac aaatgctttg gcaaaagaga ctggaaataa tagtctatcc aagggatatg
     9301 aggatgggct aaaagaagta cttaaattaa aacttgatga atatggttat gatttagaag
     9361 atatagagct taatggagca aatataaata atataaaaat aaaagaaaag aataatagtg
     9421 caaaaagtaa tagtagttcc aatgaagaaa ataaaaataa tagtactaaa agagaggaaa
     9481 aaaacataaa ctctaatgac aaagaaaatt ctaaacaagt cttcaaaaaa ggtacagagt
     9541 atgggttgaa cctaaatgaa gaaaaattaa aaaatgactt aataaaagtg cttgatgttt
     9601 ctatagaaga tatacaaatt gataaatagg agttgggaga atgttgaaaa acttaaatga
     9661 caaagataag agaaaggtat attcactaat aactatagca ggaatatgtg tggtttcatt
     9721 agtattatta tcttgttttc ctagtagcaa aagcgataaa caagttggaa aaacagagac
     9781 taataaaaat gctgaaaagc aagtcactaa agaacaagaa aaagatgatt tagagtcaaa
     9841 attaacagca atactatcta aaattgatgg tgcaggagac gtcgatgtaa tggttacatt
     9901 tgaatcaagt gaagaaatac aacctgcttt taattcaaat aatacaacag aaacaacaga
     9961 ggaaaaagat gcacaaggtg gagaaagaac agttacgact tctagtgaaa ataaaacaat
    10021 gataacttca aattcaagtg accctgtagt tataaaaact acagagccaa agataaaggg
    10081 agtgatagtg gtagcaagtg gagcaagtga cccaagtgtt aaagagacac tttatagtgc
    10141 agtacaaaca tcattacaag tagctggtca tcaagtagaa atatattcta aataaataca
    10201 tataaccggg gggataaatg aatgaagttt aattataagg gaagaggatt tgtaataata
    10261 actttaactg caatgttagt ggtagtagga acagtgaatt atcaattaag taagaaatct
    10321 ttattagaaa catcaaaaga gtttaaggca tatgaagaag cacagttaca aaaaaatact
    10381 gatgatggtg atagtagtaa gacagaggat tctagtaatg tagataaaca agatagtaag
    10441 gaaagtgctg atattgatat agttgatagc aaagctagta aagtaaaaga aaaggcaact
    10501 gagacaagta aggaaataaa agctcaatta tcatcagaga aaaatatgaa aaaggcatct
    10561 tatattttag atatgaaaat gaatagagaa aaacaaagaa atgaattagt tcaagattta
    10621 aatgaaatga taaataatcc atctacaaca gaagaatcta gaaaagaagc ttctaatatg
    10681 aaattaaata tagttaaaac tcaagaaaaa gaacttcaaa tagaaaatct tttgagtaca
    10741 aaaggatatg aagaagcatt agtttatata agtgataaca aagttaatgt agttgtaaat
    10801 gaagctaaat tagaaaagaa agatgcagca aaaatatttg atttagttgc agaacaagcc
    10861 aatgtaaagt atgaaaatat taaacttaca aataataata gtaataagta aagtataaac
    10921 ctcagaatta actgaggtta ttttcttgca aagtagactt gtatttggta ttattatact
    10981 tataaaaata gttccttaaa atcaggaggt aagttttaat atggaagata ataaatttgg
    11041 acaagtaaaa atatctaatg atgtaatagc tacaatagct ggtttagcag cattagaagt
    11101 tgaaggcata gaaactacag caacattaac agataagttg ttaaaaaata atggagtaaa
    11161 aatacaaatc gaagaagagg atgttaattt agatgtaatg gttacgataa agtatggaat
    11221 gtcaatacca gatacagctt ttaaagttca agaaaatgta aaaaatactg ttgagacaat
    11281 gacaggactg aaggtttctc aagtaaatat acacattcaa ggaatcagct ttaagaagga
    11341 taaagttgat aaagaagaag caaaagcaac taagaaaaat taatattagc cctctgaaaa
    11401 gtcagagggt tttttgctgg taattgattt tatatggata attaaactta aaggagaatt
    11461 ttgattgatg aaaaaagata gggcacaaaa gagtacaaca agagaatata taatgaaact
    11521 tatataccaa ataaatatta ataaagaaga ttttgaaact ttagaagata aagtagataa
    11581 ttttttaaaa gataattcag aacatattat aaatagatat aaagagcttg cacttcaata
    11641 ctcaaaaaat acaaatttaa agttagaaga tactgaaatt gaagatgtta tagacaaaaa
    11701 atatataaat acagtatgca aagcattgaa agaaaatcat gacaaaatag atgagttaat
    11761 aaataagcat gctaaaaact ggacagtaga tagaatgcca aaggtagatg tttctatact
    11821 tagattgtct gtgtgtgaaa tactttattt agacactcct aataaagtat caataaatga
    11881 agcagtagaa cttgcaaaaa tatattgtga tgataagtct cctaaattta taaatggtat
    11941 attgggaagt gttgttgatg aaattgggaa ataatataat aattggaatt gatactagct
    12001 gctatactac ttctatagca gctatctctt tagataagaa agttatattc aatgaaaaaa
    12061 taatgttaga agtaaaggat aattcaaaag gtttaaggca aagtgaagct gtattccaac
    12121 atatcaataa tttagggatt cttagtgata ggataaaatc ttttaaagat aagtttaatg
    12181 tagaaggagt ttgctcatca aaaaaaccga gacctgttga aaactcatat atgcctgtat
    12241 tcaatgtagg tcacaatttt ggaaaattat tgtctagtat atatggttgt aggttttatg
    12301 agactaccca tcaagagaat cacattgaag caagtttatt aaatagtaaa ttaaaaaata
    12361 ataatagatt tatttctgtt catatgtctg gtggtactac agagatactt ttgacaagca
    12421 aacaagatag tcatcacaat gtatgtgata ctaatttagg taaaattgcc aaaataagta
    12481 ttaaaaaaga tgataaaagt aagttataca ataattttgg atataatata gatataattg
    12541 gaggaagtaa agatattagt tttggtcaac ttatagatag agtaggaatt aagcttggat
    12601 ataaatttcc ttcaggaaag tatttggatg aaaatgcttt aaattgtaat cttaaaatag
    12661 aaagtggtct gaaaacttct gtaagagatg ggtatatgaa tttatctggg ttagaaaatc
    12721 aggtaaataa gattataaat gataatggag ataatactaa tcaaaaagaa tacatatcta
    12781 aattagtatt agattcagta gttagaaata tgtttaaatc tttagtatat ttatgtgaaa
    12841 cttataatgt aaatgaagta gtatttgctg gtggagtttc tgcaagtaag tatattctta
    12901 gagaattaag catgaagtta agaaaaaagc atatagaagc ttattttaca gaaccacaat
    12961 attcaactga taatgctgtc gggtgtgcta taataggatt gaataacttt ttaggagaaa
    13021 gagtatgaaa ctaagagctt tagatataag tgaagcaaac tcatatatta aaagaatttt
    13081 aataaacgac ccaatacttt ctaatcttaa agttaaagga gaaatttcta attttaaggt
    13141 tcatagtagt gggaatgtat atttatcact taaagatgaa acatcaaagt taaactgtgt
    13201 aatttttaaa agtaatttta atcgcaattt aaaattagat aatggtgtaa aagttatagc
    13261 taatggatat atatcagtct atgaaagaga tggtgcgtat caattataca ttaatgaaat
    13321 tgaaatagag ggaataggaa atttacacat agagtttaat agattaaagg aaaaacttaa
    13381 taaagaagga ttatttgacc ctaaatacaa gatacctata cctaaaatgc caaactcaat
    13441 tggcgttata acatctccaa caggtgctgt tataagagat ataataaatg taataaaaag
    13501 aagatatccg aaagttaata taaagcttta tccagtaacg gttcaaggag ataaatcagc
    13561 tgaagaaatc tgtgaagcaa ttagattttt caatcatatg aagaatgttg atacacttat
    13621 agttggtaga ggtgggggtt caatagaaga actttggtct tttaatgatg aaatggtagc
    13681 aagagaggtt tttaattctc aaattccgat aatatcagca gttgggcatg aaacggattt
    13741 tactatttgt gactttgtgt ctgatatgag ggctccaacg ccatcagcag cagctgagat
    13801 tgctactcca tcattggatg atataaacta taaacttgga aacataaaaa gcaggatgag
    13861 taagtctctt acaaatcaaa tagagctaga ccaatatagg ttagaaacag tatttaataa
    13921 gataaataac tatttagatt catacacaat aaaagataaa gttatacaat tagacaaaat
    13981 atatgataaa ataatttttg gaatagaaaa taatttaaaa ttagaagatg aaaaattagt
    14041 aaaaattgga gcattactcc ataatttaag tccattagct actatggata gaggttatag
    14101 tataactcaa aaaaatggaa aagtaataaa tagtataaaa ggattaaaaa taaaggatag
    14161 tatagatata gtattaaaag atggaaattt agaatgtatg atagataaaa ttgaaaataa
    14221 agagggatag tatgaattta acctacgaag aagcttataa aagattagaa agtattttaa
    14281 gtgaattaga atctaaaaat gctagcttgg atgaatcttt aagcctatat gaagaaggta
    14341 taagccttta taaacattgt aataagcttt tagatgatgc taaattgaaa ataagtaaat
    14401 ttaatcaatt aggaatagaa gaagacttta aaatagagga ggaataaaat tggaatttaa
    14461 acaatgtcta aaagaaaaag caagttttgt agaaaaagta cttaaagaat atatgcctaa
    14521 agaagaaggg tatcaaaaaa ctgttattga agctatgaac tatagtttaa gtgctggagg
    14581 taaaagatta agaccaatac ttacattgga ggcttgtaaa atagttggag gaaatgaaga
    14641 tgaagctatt ccttttgcta ttgcaattga aatgatacat acatattcac taatacatga
    14701 tgatttgcca gctcttgata atgatgattt aagaagggga agaccaacaa atcataaagt
    14761 atatggagaa gctatgggga ttttagcagg agatgctttg ttaaactatg cttttgaagt
    14821 aatgcttgca ggctcaatca ataaagaaaa tccagaaaag tatctaaaag ctataaatga
    14881 aattgccaaa ggtgctggta tatatggaat gataggtggt caagtagtag atgtagaaag
    14941 cgaaaataaa caaattgaaa aagaaaaatt agattatata catatgaata aaactgctgc
    15001 aatgatggta ggatgtatga gagcaggagc tactataggt ggagcaaatt ttgaacaaat
    15061 ggaagaaata actaaatatg caaaaaatat cggattatca ttccagatag tagatgatat
    15121 actagacata gtaggagatg aagctaagtt aggtaaaaag gttggaagtg atatagaaaa
    15181 tcataagtca acatacccat ctcttttagg tttagataaa tctaaagaaa ttgcacataa
    15241 tttgattgat gaagcaaaga aaagtataga aaaattatct gatgatgtag attttttaaa
    15301 gggattggct gaatatataa tagatagaga atactaaaat atacagctaa aatcgaggag
    15361 ggaaaaatgg attttttttc ggagattttt aacaatgggg ctttgggaat aagtctgatt
    15421 gcttgtttct tagcacaatt tataaaaata tttactggta aggaaaaaag aattgagtta
    15481 tcaagaatac ttatttcagg aggtatgcct agttcccata gttcttttgt tacaagttta
    15541 gctacagtgg taggtattga aaaagggttt aattctactg attttgctat aattaccgtg
    15601 cttgccttaa ttataatgta tgatgcagca ggtgttagac gggctgttgg taaacaagct
    15661 actatattaa atcaaatggt ggcagatata cagcatggaa agcatataga gcagaagaaa
    15721 ttaaaagaat taataggtca tacaccacta gaagtttggt ttggagcttt acttggaata
    15781 gtaacagctt taatattgat gtaaattgaa aatttacaga ataaaggtga ggataatatg
    15841 tataaatatt tagataaagt aaattctcca aaggatataa aaaatatgag tatagaagaa
    15901 atggatttac ttgcaaagga tataagaaag ttcttagtaa aatctgtttc aaagacagga
    15961 ggacacttag cttctaatct aggagtggtt gagttaacat tagcacttca taaagtattt
    16021 gatagcccta aagataagat tgtatgggat gtagggcatc aatcttatgt tcataaaata
    16081 gttactggaa gaaaagattg ttttgtatct ttgagacagt ttaatggact aagtggtttt
    16141 ccaaaggaga atgaaagccc tcatgatatt tttgatacag gacatagcag tacatctatt
    16201 tctatagcta caggtattgc ctgcgctagg gatataaaaa aagaaaatta tagtgttata
    16261 tctgtgatag gagatggttc tataacagga ggtatggcac ttgaagcttt aaatcaatta
    16321 gggtatatag ataccaatat gatagtaatc cttaatgata atgagatgtc catagataaa
    16381 aatgttggag gaatgtctaa atatctgtca agtattataa gaaactcaac agttgagaaa
    16441 atgacagatg aagttgataa aattttaaat gttacacaaa caggtgaaat tttatctaag
    16501 actgcacata gatttaaaga taaactaatg tatagctttt ctcctcagga ctgttcattt
    16561 tttgattcat taggtataag atattatgga cctatagatg gacataatac taaagaacta
    16621 atagatatat tgagaaaagc taaacacaaa aaagggcctg ttcttttaca tgtaattacg
    16681 aaaaaaggaa aagggtacag atttgcagaa gaacaacctg ataaatatca tggagtatca
    16741 aagtttgata taaagacagg tgttacatct gcaaaagtta agtctatgtc aattagtgtt
    16801 ggagaaaagc ttgttgatat ggctaataat aatgaagata tagtagcaat aacagctgca
    16861 atgccatctg gtacagggct aaacctattt gaaagtgcat atccaaaaag atattatgat
    16921 gttggtatag ctgaacagca tgcaacagga tttgcagcag gtcttgcaaa aaatggtatg
    16981 aaaccttact ttgctgtgta ctcatcattt ttgcaaaggg catatgacca agttattcat
    17041 gatgtatgca taacaaaaaa accagttact tttcttatag atagagctgg attagttgga
    17101 aatgatggag aaactcatca tggtatgttt gatttaagtt acttaaattc tattccaaat
    17161 attgtagtaa tggctccaaa agatacaaga gagatggagc ttatgatgga tttgtcatta
    17221 aaattagatt gtccactagc aattagatac ccaagaggaa gtagttatta cctagataag
    17281 ggagaatatg gagagattgt actgggtaaa tatgaggtat tagatgatgg tcaagataca
    17341 gtcatacttt gtattggaag tatggtaaaa catgccttag aggctaaaga aatattatca
    17401 agagaaggaa taaatccgac aatagttaat gctagattct taaagccaat tgatgaagat
    17461 atgttaaaag tattattaaa aaatcataaa aatgtagtta ctatagagga taatattgta
    17521 actggaggat ttggaagtag aataaataag tttattatag ataatgaata taacgtaaat
    17581 atacttaata tagcaatacc agaagaattt gtaaaacatg gcaatgcaga tgaattatat
    17641 gattttgtag gattatcgcc aaaaagtatt gctgataaaa taagaaaatt agttattgag
    17701 taaaaatcta atgtagatat tgtaaattaa aacttatata aaatatagaa tttatcaatt
    17761 agaataaata tcaaatgtgt tacttcaagc tactttagtt atcattaaag tagcttttaa
    17821 cagaaaggta attgtatgaa aaaaagaata gatttattat tagtagaaca agggtacttt
    17881 gaaagtagag aaagagccaa aaaagcaata atggctggat tggtttttgt tgataatcaa
    17941 aggtgtgata aggctggtac agaagtaaaa gaagattgta gtatagaagt aaaaggaaat
    18001 ccaataccat atgtcagtag aggtggttta aagcttgaaa aagcaatgaa aaactttgat
    18061 ttgactattg atggaaaagt atgtatggat ataggggcat ccacaggtgg ttttacagat
    18121 tgtatgctca aaaatggagc aataaaagta ttttctatag atgttggtta tggtcaactt
    18181 gcatggaaat tgagacaaga tgatagggtt gtgtgtatgg aaagaaccaa tataagaaat
    18241 gtgacaatag aagatacaaa gcagtttgct gattttgcgt caattgatgt gtcttttata
    18301 tcattaaagt tagtacttcc aaaggcaaag gagttagtac gtcatgatgg ggaaattgtc
    18361 gctttaatta aacctcaatt tgaagcagga agagaaaaag taggtaaaaa aggagtagtt
    18421 agagaaaaat ctacacatat agaagttata aaaatgattt ctgatttctc agtcgaaaat
    18481 ggatttgaga ttttagggtt agatttttct cctattaaag gtcctgaagg taatatagaa
    18541 tatctaattc atttgagaaa tggaaatgaa ggatatgaat ttgatggtga gatatacaat
    18601 aacaaaatag tagaagttgt tgaagcttct cataatttag acaaataggt taaaaggggg
    18661 cttagatttg atcctcgaat tatatatgaa aaattgtgcc ttagttgaag aactaaggtt
    18721 gaatattgat aaaaacttaa atatacttac aggtgaaact ggttcaggaa aatctataat
    18781 aatagatgca cttggacttt gtttaggtga taaatatgat aggtcttttt taagaaaagg
    18841 aacagataag ggacttgttg aagctgtatt tttttcagac aatatatatc ttaaaaaaat
    18901 attagaagaa aatgatataa gtatggaaga tgataattta ttggtaataa ctaggttgat
    18961 atactcagat ggtaagagta ctgctagagt aaatggaaga actgttaaag tgtctcttct
    19021 taaagaaatt gcatctacac ttatagatat acatggtcaa catcaaaatc aagctttatt
    19081 taataaagat actcatttaa aatttctaga cctttttgga gaaaatgaat tggaagggtt
    19141 taaaattgct tataaaaatg tgtaccataa gtatagtgaa gtaaaaaaag ctttaaattg
    19201 cttgacagaa aataaagatg agatgcaaat tcaacgagaa atagatttat taagatttca
    19261 aataaatgaa attgaagctg caaatctaaa taagaatgaa tatgaagatt tattaaaaca
    19321 aagagaagtt tatagaaata gtgaaaagat atataataat ctaaattcga gttatagcaa
    19381 gttacataat ggtgagtata atgttattga cctaataggt cttgcgtcta aagaacttaa
    19441 tgacatatct aaatatgaca gtgttttaag tgaatatagt gatactgtag aaagaattat
    19501 gtatgagtta caagacatat ctggtgagat aagaaattac aaagataata tagactttga
    19561 accttatgag ctagagcaga ttgagcttag aatcgacgag attaataact taagaagaaa
    19621 atatggagac agtatagatg atatatttga gtattatgga aaaattaaag atagattaga
    19681 tgaaatatta aatagagatg aaagagttga acagttaaaa agcaaactga tgaatataga
    19741 agaagattta aaaataaaag ccagtaaatt gactaaagca agaatagaag tagcaactac
    19801 tttagaaaaa atacttcttg atgaattaaa aagcttaaat atgaaaaatg taatgttcaa
    19861 ggttaatttt gaaaaaggtc tatttacttt agatggagta gatgatattg aatttatgat
    19921 atcttttaac ttaggagaag atattaagcc tatatataaa gttgcatcag gtggtgagat
    19981 gtctagattt atgttagcat ttaaaaccat attggctgat atagatgata ttgatacatt
    20041 agtgtttgat gagatagata ctgggataag tgggatagca gcacagattg ttggtgagaa
    20101 attgagtgat atagcaaaga aaaaacagat aatatgtata actcatttac ctcaaattgc
    20161 tgcaaatgca gacactcatt attgtataga gaaagatact tcaaataata ggacgtttac
    20221 caatgttagt aagttaaatg aatctcaacg aaaaaatgaa attgccagac ttatagcagg
    20281 taacaatata acagaaaaaa ctattgaaca tgcaagtgaa atcattgaat tggctaaaaa
    20341 atgttagtga aaaacaagca ttttaatagg tgtaaagaat ttttatagaa atgctttaca
    20401 cctatttttt tatgatgaaa ttattttaaa atatctagtg cctatattga agaaaagtag
    20461 gtttgaaaat ggaatgaaat ttgtataaat ttgtaacttt gaaatttcta aatgcttaag
    20521 tataatatta tttattaagg tagtagtgag gggaggcata aaatgaataa aaaagatact
    20581 atgcacttaa tttttataat tgtaattttt attttattat ggtatattgt ggtagcagta
    20641 agtgagatta ataaaaatat tgaaagttgt agtaacaaat tagattattt atcacaagag
    20701 attatgagca ctaagtcgga tattggtaat actataaatg aagaaatgtc aaaaagttat
    20761 ataacaaaaa gtattgactt aaaagttaaa aaaatcgaga aaaaagagtg tattattgat
    20821 atagatgttc aattaagtaa acttggaaaa aatggaaaag tattctttat gtataaagaa
    20881 gatagtgata aatggaatga aactgagatg acaaaacatg gagaattatc atatgcatgt
    20941 gagataaaga taaatacagg aagtgaatat gactataaag tagttacaag tggagctata
    21001 tctgaaagtt ctgatgtgca aaaattgact aaatctgatt atatgcctga acaacccata
    21061 tttaattctg ggataagaga taatagagaa tactttattg aaatagtaga aaatagtaat
    21121 cttttcaatc atgagagtga aaagtcaaaa aataaagctt atgataatat tagattagaa
    21181 aaagtagata ttattgtaaa tgaaggaaaa aaagacacta tatacaaagc caaattagct
    21241 gaaggcttag atgatggcaa aactaataat tcaaatagaa atcaagtgaa ttatgaagta
    21301 agttttccta agagggatat tgatgacata atttatatta aagctaaact tatgtacaat
    21361 aatggagtag aatatacaaa ggatataact gaagatataa ctatgggatt acaagattta
    21421 gaaaaataaa tacatattta aataaatatt caaatagatt tgttcaataa aatagagtat
    21481 aagtaaaact gtagtagttt tacctatact ctattaaaaa attataattt taattccata
    21541 tatctagccc cttcaattgg attataagta taaggttcta tatcataaaa accaaacttt
    21601 tcatataatc cttgtgcact tttcatgctt ggaagagtat ctaatctcat gtgtgtatat
    21661 ccaatttttt tagcttcttc aatgatttct tccaatagta tttttcctat ttttaatcct
    21721 ctaaattgat ttcttacata taatcttttt aattcacata ttttatattc aagttttttt
    21781 aaagcaacac aacctgccaa attttcatca acaaatgcca atatcaatga gcctgaaggt
    21841 tttttatatt ttcctggaag tgtttttaat tcattattaa agtcttggaa acataagtct
    21901 atatttagag aatttgaata ttcagtaaat aaaagtttta cgttttcaag gttttctaaa
    21961 tctttttctt caacaaattg taaagttata ttttgagcca ttttatccgc cattatataa
    22021 acatgtttat ataatgactt cacctccttg atatatgtat atttttctta atatcaatta
    22081 gattttaaca ccattacttt ttcaccacca tagtcaagct ctccattaaa ctcaaatcca
    22141 aaatctttat aaagcttaat agccatttta ttgtcttcga atatactaag atatagttca
    22201 tcacagttat attcattttt aagatgattt acaagaaaat ctaatgatgc ttttccatat
    22261 cctttacctt gatattttga atcaatcata aatctatcaa gccaaactct atcatcttct
    22321 aaacgaccat acatagcaaa tcctattaaa gaattttcat catatattcc aacaggtatc
    22381 caagctgtta taaatttaga ttcagcaata gaaagtgcat tgctttctat ataatctaac
    22441 tgacttttat ctacagacaa actaactaaa tcatgccaat tatttatgtc tgcaattctt
    22501 acatttaatt tcaagatatt accgcccttc aaactttttg ttacaatata atactatcat
    22561 attatgtatt aaaaatcact tattaagatt ttaaataaac tttttgaatg aataaattct
    22621 caggaaaaag gaattataaa tacataataa tacagcatag gagtagatgg atatatgaac
    22681 aaaataaata aaaatttcaa aaatatttgg ataatattag gagctgtatc gatttttacc
    22741 atggttataa tttttaataa aaattgtaat gttaataaag aaactcaaat agaagctttt
    22801 acaattaaga atcatgataa aaaatttgta tatcctatgg gaaatataat tggagtaaaa
    22861 gcaaatacag atggtgtatt ggtcttagga tatgaggaag aggatataga ctatattgga
    22921 ggcatacaaa tcggtgataa tatagtaaaa atagataata aaagaataaa aaatagtcaa
    22981 gatgtatcag agattttgaa taagattaaa aaaaataaag ttgaagtgac ttttgagaga
    23041 aaaaataagt ataaaacaga agtaatagag actaaaaaag aaaatggcaa gtataaactt
    23101 ggattatggg taagagataa aatatctgga attggaacta tgacttttta cgaccctagt
    23161 atggaaaagt tcaaagcaat cggtcatgcc ataaaagatt cggatactaa tgaactatta
    23221 aagataaagc aaggatatat atataagcca gaacaattaa aaatagtaaa agctagtaat
    23281 gaaaaagtag gtaaaataaa aggagatttt aatgacagca atttaatggg taatttctca
    23341 aataattcag agctaggtat aagtggtaat attatagaaa accataataa agaatttaat
    23401 gtagcaaata aagaaaagca attaatagaa gttggaagac cacaagatgt aaaaatagga
    23461 gatgcagtaa ttctttttga ggataaaaac aaaaatataa caagctatga tataaaaata
    23521 gaaagtatag tgtatgataa aggaaattat agagatatgg taataaaagt agtagatgac
    23581 aagttattgg aatacacagg aggtatcgta caggggatga gtggagcacc aataatacaa
    23641 aataataaaa ttattggtgc aataactcat gtttttagag ataatccgaa aaaaggatat
    23701 ggtattttta tagatgaaat gataaaattg taggtgaggc attaaaaaat tttattattt
    23761 tatcaattat ctaggaggaa tataattttg gagtgtcgaa tatgctttag agtagataat
    23821 taggaagcaa ttgtgtaaaa agtttagttt tctgtaataa gaagatgttt tttaatgggg
    23881 ggatttttag tggaaaaaat caaaatagtt ttagcagatg acaataagga tttttgtcag
    23941 gtattaaaag agtatttgtc taatgaagat gatatcgata tattaggcat agctaaggat
    24001 ggaattgaag cattagactt agtaaaaaag acacaaccgg acttattaat actagatgta
    24061 ataatgccac atctagatgg attaggtgta attgaaaaat tgaatactat ggatatacct
    24121 aaaatgccaa aaataatagt actatcagca gtaggtcaag ataagataac tcaaagcgca
    24181 ataaatctag gagcagatta ctacatagta aagccatttg attttgttgt gtttataaac
    24241 agaattagag agctagtttc taatagagtt acacaagttg agccaaaacc tagaccagtt
    24301 caagagactc aaatgactag aagtgatttt gttaagaatg taggaaatat agagacagaa
    24361 atcacaaata taatacatga aataggagta ccagctcata taaaaggata tttatattta
    24421 agagaagcaa taaaaatggt tattgataat gttgagcttt taggtgcagt aacgaaagag
    24481 ttatatccaa gtatagctaa aaaattcaat acaacaccaa gtagagttga aagagcaata
    24541 agacatgcaa tagaggttgc gtggagtaga ggaaaagttg acacaataaa tcaattattt
    24601 ggatatacgg tacacaatac taaaggaaaa ccaactaatt cagaatttat agcaatgatt
    24661 gctgataaat taagactaga acatagtatg gttaaataaa caagacataa aaagtaaggc
    24721 tttttaatta aggcattggc tataaatgcg tattacaagc agcgaaacgg tataaccact
    24781 agggttatac cgtttcgcta ttttaataaa tataaaaatt ttctttatta tttgcttact
    24841 atatcaatat aataatttta ttatactatg gatatagtat gtgtctttac aagttgtaaa
    24901 ctgacagtgg tttatttttt aatataaata ttgactttga tgtaggtaaa ctttgcattt
    24961 ttaagcatat tatggaatat gttaaataaa aaaatgatga aatatagtat tgtaaatgcc
    25021 aaagatgcga aacaaactta aaacatttat tttattgtta agtaatgcta taatataatg
    25081 tgattttaat aatgatagtg gaggtttaaa tatgagagtc gaggccccta taaaagtaga
    25141 tcgaaaaacc aaaaaacttg ctaaaagagt tgaaagtggg gaaatagcag ttataaatca
    25201 tatagacata gatgaagttg ctgcaaactc tttagtagaa gctaaaataa aacttgtcat
    25261 aaatgcggct ccttctataa gtggtaggta tcccaataaa ggtccaggga tattaactga
    25321 aaataatata ctaataattg ataatgttgg agaagaatta tttgaagaat taaaagaagg
    25381 agaaactata gaagttgtag atggaaagat atatagaaaa ggaaaatttt taggtgcagg
    25441 agaagtttta gacaagtatg aggtttcaca ccaaataaag gctgcctatg agaatttagc
    25501 tgtagaattg gatagattta tagataatac tatagattat gctaaaaaag aaaaaggatt
    25561 tatacttgga gaagtagaaa ttccaaaagt gaaaactaat tatgctaata aacatgtatt
    25621 gatagttgta agaggtcagg actacaaaga agatttaagt actatgcttt cttatataga
    25681 agaggtaaag ccagtattgg ttggtgttga tggaggagca gatgctttaa ttgagtttgg
    25741 atatactcca gatgtgattg taggtgatat ggatagtgta agtgatgaag ctttaaaaaa
    25801 ggctagtgaa atagtagtac atgcttatac agatggaaga gcaccaggtc ttaagagggt
    25861 tgaggaatta ggcttagatg cagttgtatt tcctgcgcca ggaactagtg aagatatagc
    25921 tatgcttatc gcttatgaat ataaagctga gttaatagta gcagttggta cacattcaaa
    25981 tatgattgat tttcttgaaa aaggaagaaa aggtatggca agtacttttt tagttagact
    26041 aaaaataggt tcaaagctta tagatgctaa aggagttaat ttgttataca gaagtaaatt
    26101 aaaaataaag tacatatggg cattaatagc aacagcatta tttcctgtgt tggtagttgc
    26161 atcattgtca ccaggagttc aacagttcat acaactaatg caattaaagt ttagagtatt
    26221 attacagatg taaattttag gaggataaat atgcatatta atatgaaata ttatatagtc
    26281 actatagggg ctatatttat agctttgggt ataggcatgt tagtaggatt taatttaaat
    26341 aataatcaac aattaagtga acagcaagca aacataataa atgatttgga tgataagttt
    26401 aatatcttaa aagaaaaaaa tgataaactt gacagtgact tagcaagtgt aaatagagat
    26461 tatgaagagg cagttaattt tataaataaa aatgtagata agattttagc tggttcgttg
    26521 aatggtaaaa atataggaat aatatcggct aatgaaaatg acgattatac aaaaaatata
    26581 gaagacataa taaataaatc taatggaagt attgcattta atatagtatt aaaagaaaat
    26641 ataacaaatc cagaaaagtt aaaggaaatg tcaactaaac taggtacaga tgtaaaaaat
    26701 gcaaatgatg cagttaatta tataattgac actcttaaaa aggaagatgc aagtgacatt
    26761 ttaacatatc ttcaagaact agatgtaata aagtttaact ttattggaga tacatatcta
    26821 aaatatgatt ctgttgtaat agctggtgga aatgatgcaa aagacaatac aaaacagttt
    26881 gaaaaaatag aaaaatttgt agtttcaaaa cttaaatcag agaataaata tttagtagaa
    26941 gttcaaaata caggagttaa aacttcttat gtagaactat attctaaaaa caaagttgca
    27001 actatagaca atattgatga aggtatagga agtatatctc tagctattct tctacaacaa
    27061 ggtaatatag ttggtaattt tggtagattg gatactgcaa cttcattgtt gccatctatt
    27121 aaatagaaat aattttaagg agagaaaaaa tggattctta tataagtata ataataccag
    27181 cttataatga agaaagtaga ataaaatata cattagaaag catattggac attaaacaaa
    27241 taaacgaaat aatagttgtg gatgatggtt cgattgacaa tactgctaaa gttgtatcta
    27301 aactagaaaa tgaaaagatt aaatttttta agttagataa aaatagaggt aaaggatatg
    27361 ctttaaatta tggtatcaag ttagcaatga aaaatgcaga tattatagga tttctggatg
    27421 gagatttagg aagctctgct aaagaagttg aaaaattaat agtacctata ttaaatggtg
    27481 aggcggatgt tacaatagcc aaatttcctc ctgcaaagag aaaaggaggt ttgggatttg
    27541 taaaaggttt agctaaaaat tctgtgtttg agatgactgg tgttgagtta gatgcaactt
    27601 tatcaggtca aagaatattt aaaagagaag ttttagaaaa gtttgatgaa ataccctttg
    27661 gatatggagt agaagttgga atgaccattg atatattaaa gtatggattt aaaattaaag
    27721 aagtattagt aaatatgact cataatgaaa caggaagaaa tttaaaaggt tttattcata
    27781 gagggaaaca gtattatcac ataaaaaagg ttttaggaca aaaaaagaaa gaatggaggt
    27841 aatatcttag tgaaagatta tatactttat accattttat tattaactgg attatttgga
    27901 acctatgctg taattccact atttaaaaat ttattgataa atggaaatgt acttagacca
    27961 aactataaaa aagatatgat accagtaagt atgggaatcg tatttttgcc tatgattgta
    28021 atcaatggaa taatacttgc tttctttact acagaattta aggatttagc gtatatattt
    28081 atgtttatgt ttggtatgat atccatgttt tttgcaggga tactagatga tgtcattgga
    28141 aatagagatg taagtggttt aaaggggcat tttaaaagtt tatttaaagg aagtcttaca
    28201 acaggaggtt ttaaagcttt atttggtgga tttgtagggc ttatagtttc tgttgctata
    28261 tcaaaagaca taattgatat aatagtaaat acactaataa tagctctatc aactaattta
    28321 atgaatcttt tggatttaag accaggaaga gcaataaaag catatttatt cataatggta
    28381 attatatttt taacattgac aggatttgtt caagttcttc cattacttat agtaccaaat
    28441 gtacttgcat actttaatta tgatttaaaa gcaagggcta tgatgggaga tactggttca
    28501 aacgtccttg gaatatctat tggaatgctc atttcttttg ggtatacatt taacattaga
    28561 ttaggatggt taatatttgt agttattatg catatattca ctgagaaata ctcattgact
    28621 aagataatag aaaaaaataa actattaaat tttatagata aattgggaag gtagtattat
    28681 gataagcaaa agagtgggga ttgttgaatc aatagtaagt caaactgaaa ctctagatga
    28741 tataagagta aatataaatg gagaaataca aagggcatat aattatccaa aaatatctgg
    28801 aactataaat ataggtgatg aagtggtttt aaatacaaca gcagtagaac tgagcttagg
    28861 tacaggtgga taccattttg taataactaa tttaaataat atagaatcta ctttaactga
    28921 aggtggccat ataatgaaac ttagatatac acctcttcaa ataaaagtgg attctgttga
    28981 ggaacaagaa agtatttatc atgataaatt tgcgaatttt aaaggattag aaggtctttc
    29041 tgttgtagta gggactcttc atagtatgtt aactccattt gcagcttcat ttaaaagaaa
    29101 taatccaaat aaaaagttag tatatataat gactgatgga gcatctcttc caatttattt
    29161 aagtaaaaat gtggatattt taaaagaaaa aaaattaata gatagtacga taactatagg
    29221 aaatgcgttt ggtggagatt atgaatgtat aaatatatat actgcattaa ttacagcaaa
    29281 agaaatacta aaagcagatg cagtatttgt aagtatggga cctggaattg ctgggacagg
    29341 aacaaaatat ggatttacag ggattgaaca aggctctatt cttgatgctg taaaaaaact
    29401 tgaaggaaga gcaattgcaa ttccaagaat tagttttgct gacaaacgag aaagacatca
    29461 aggtatttca catcatagta tgaccgtatt taaagaaatt gtaaatgtaa atgtagacat
    29521 accaataact atatatgatg atgaaaaatt gaactttata aaagagcaaa tcaatttaaa
    29581 tggtttagac aaaaagcata atataattta tatagataat aataaaacga aagaggattt
    29641 agactatttt aatctaaaag taaagagtat gggaagaaat tatgaacaag atgaggcatt
    29701 tttcaaagct gcaagcacag cagcatatta cttaatggag gtttaagatg gtattagaag
    29761 aaaaaactat tagtagtgat agggtttata caggtaaagt aattacttta aaggttgata
    29821 ctgttgaaat tccagggcaa ggatatcaaa aaagagagtt agtagaagtt ggtggagcag
    29881 taggaatagt tgcaatcaca gatgataata aagttgtgct agttaagcaa tttagaaaac
    29941 ctatagaaaa gccaatcttt gaaattccag ctggtaaact agaaaaaaat gaaagtccaa
    30001 aagaatgtgc tgaaagagaa ttaaaagaag aaactgggta tagtgcaaaa aatattaagc
    30061 taatacataa atttttcact tcagcaggtt tttctaatga gataatgttt gtatatttag
    30121 caacaggtct tactccagga gagaataatt tagatgcaga tgagttttta gatgtctatg
    30181 aaattgagct tgaagaagca tataatatgg tattaaaaaa tgatgttgaa gatgcaaaaa
    30241 catctattgg attattatta gtaaaagata tgtttaaaaa ttaatattta taaaatgaat
    30301 aaccctttgt cctaaagcat attattttat aaaggacaag ggggaattaa attgagaaga
    30361 acatatagaa aaaatgattt tagagaatta aatagacaaa taattattat aggattgttg
    30421 tttatgatgt ctattgttgt tgggtcttac ataaataaaa tattgccagg ttctagtaac
    30481 aatatattaa acaatataaa tcctgctgta gagtattata atctaaatat aagcataaaa
    30541 gatacagtga tacaaaattt aaaatcagat gcagtcttta tgggctcaat agctctatta
    30601 agtctatttg tagtaacaat tccagcagtg ttggtagctt ttgttttaaa agggatgtca
    30661 attggttata caataaatag ttgtatattg gcattaaaat ttaaaagtat aaaaatgatt
    30721 ttaataatat tatttaaaaa tttgattatt attccatgtg caatcatatt agcgttaatt
    30781 agtttaagtt attttaaaga aatggtttat gaatttaaaa agaaaaatag aaaaaatatg
    30841 caatttttaa ttaagaggta tatactaaat attataatta taatagcact atcattgggg
    30901 ttgcaattaa tattaaatac tgcaagtatt ggtattatta agttcctagc taaataaagg
    30961 agtttataat ggatattata gagggatata tagattacat aaaagataaa aaaaaattat
    31021 cagagaatac agtggcatca tactttatgg atataaaaaa atatatggaa tatataaatc
    31081 aaaaaagcat taaactagta gatatagtag aaaatgatat tataagttac ttaataaagt
    31141 tggaaaaaga taatgtatca atagctacaa tagcgaggat gatatcctct ataaaatctt
    31201 ttcatgatta tttattttta aatcatatat gtacaaataa tcctgcaaag gatataaaaa
    31261 aacctaaaat aaaaaaagaa aatataaata tacttacaga agaagaaatt gaaagactat
    31321 taaactttcc aaagttaaca acacctaaat tgattaggga caaggctatt tttgaagtat
    31381 tatatggtac tggtataaaa gtatctgaat tagtagagat gaatatagag gatattgacc
    31441 tagatattga ttatatatat tgtaattcat gttgtaaaaa tcaaagagtg ataccacttt
    31501 gtgatattac aaaactttac ttagaaaagt atctaaaaga agcaagacct aaaatggctg
    31561 tagaaggtga aaaatcatta tttgtaagct cattaggtca aaggtttaca cgacaaggtc
    31621 tttggaaagt cataaaaaaa tattctaatt tagctaatat agataaaaat ataaatccta
    31681 caatgcttag acattcattt gcaattcatt tgttaaatga aggtgctaac attgctgttg
    31741 taagtaaaat tttaggaaat gttaatttat ctagtttgca agtatattta aatcatatag
    31801 ataaaaacgt gagaagagaa ataaaggaaa aacatcctag aaatgatgtt gatgtagaat
    31861 tacaaaaagc agaagctaaa tagactatat agcaaatttt ataatttaga aggagcatat
    31921 ttgtatgagc agagtaattt ggattgtaat tgatagtgtt gggataggag cattacctga
    31981 tgctgaaaat tttggagata gtaaggatgt aagtactctt ggaaatatat ttaagaaaca
    32041 tccagatatc cagattccta atatgagaaa attggggata ggaaatatag atggtgtaga
    32101 tttttttgaa agtataaaag aaccaatagg ttgttttgga aaatgcaagg aaatgtctca
    32161 gggaaaagat actactacag gtcattggga aatgactggt ataatagttg acaaaccctt
    32221 caagacattt gaagatggat tctccaaaga gataatagaa gaatttgaga aaaaaactgg
    32281 aagaaaagta gtaggaaata aaccagcttc aggaactgta ataatagatg agtatggaga
    32341 gcatcaaata gaaactggag atgtaatagt atatacatca gctgatagtg tatttcaaat
    32401 agctgctaat gaagaagtaa ttcccctaga agagctctat aatatgtgta aaatcgctag
    32461 agaaataatg atgggggata atgcagtagc aagagtaatt gctagaccat tcataggtaa
    32521 gaaaaaaggt gagtttgtga gaacttcaaa tagaagagat tactcattag acccatttga
    32581 accaacagtt cttgataata taaaagaatc aggacttgat gttttagcag ttggaaaaat
    32641 agaagatatt tttaatggaa aaggaattac agatgctatc cacactaaaa gtaatatgga
    32701 tggagtagat gaaactttaa attatatgaa acaagataat aaaggtctga tatattcaaa
    32761 tcttgttgat tttgattcta aatatggtca tagaagagac ccagagggat acaagaaagc
    32821 acttgaagag tttgatagta gacttcctga aataatggct aatatgagaa aagatgatat
    32881 tttaataatc aatgcagacc atggaaatga cccaacatat aaaggtacag accatacaag
    32941 agaatatata ccagttatga tttatggcaa taagattaaa aaaggtttta atttaggagt
    33001 aaaagatact tttgctgata ttggagcaac tgttgcagat atactgaatg taaagttgcc
    33061 taaacatggg tcaagcttta aagaagattt attttaaatt agaaaatgat aaaaacttag
    33121 gttaatagga tggagagatt ggaatgtttg aaaagatagc acaaagtagt gaatttataa
    33181 attctaaaat taatgtaaaa cctaaaattg gtttgatatt aggttctgga ctaggggatt
    33241 tagcaaatga tatagaagac tctgtcacaa ttaaatatag tgaaattcca aacttccctg
    33301 tatcaacagt agcaggacat gctggacaac ttgtcattgg aaagcttgaa ggaaaagaag
    33361 ttattgctat gcaaggtaga tttcattatt atgaaggcta ttctcaaaaa gaagctactt
    33421 ttccagtaag agttatgaaa gaattaggag ttgagattct tatagtaact aacgctgctg
    33481 gaggcgtaaa taaagaattt aaagcaggag atttgatgct tataagagac catataaact
    33541 ttagtggaag taatccatta gtaggaaaaa atgatgatag atttggtgct agatttccag
    33601 atatgtcgga tgcatattct tctaaatatg tagatgtagt taaaaactgc gctaaagaat
    33661 gtaatataga tgtcaaagaa ggcgtttata tgttttttag tggtccaaac tatgaaacac
    33721 cagcagaagt gagaatggca caaattttgg gagctgatgc tgtaggtatg tctactgttc
    33781 cagaggtcat agtagcttct cactctaata taggagttat tggtatatcc tgtataacaa
    33841 atatggctgc tggcatatta aatcagccgc taagtcatga ggaagtaata gaaactactc
    33901 aaaaagttaa atcagaattt atgaatttag ttaaaacaac agttaaaaat ttataagtta
    33961 aagattatta tagatttttt gagcaagttt ataataaaat agttaatgtt taatttgaaa
    34021 agaattaata aagagctgtc ttgatataga aagttatttc taataaagga cagttttttt
    34081 ataatactac aacatttgaa agtgttttaa agtttaatat ttatttaaat tattataggt
    34141 tagaatggag atttcaatta tgagaatgta tgatattata aaaaaaaaga gagataacct
    34201 agagcttagc aaaaaagaga taaactactt tatagaaaac tattcaaagg gagatatacc
    34261 tgactatcaa gcttctgcac tattaatgtc gatttattta aataagatga ataaacagga
    34321 aactgtatat cttacagaag caatgatgaa ctctggtgac atgataaatc tatctgagat
    34381 agatgggata aaggtggata agcatagtac tggtggtgtt ggagataaga ctactatagc
    34441 tcttatacct ttggttgctt catgtggagc accagttgca aagatgtcag gcagaggtct
    34501 tggtcatact ggtggtacta tcgataaact ggagtctata ccaggttttt cggcagaaat
    34561 ggagataagt aagtttattg actccgtaaa taagtccaaa atagcagttt gtggtcagag
    34621 tgcaaaagta gcaattgcag ataagaaaat atatgctctt agagatgtta cagcaactgt
    34681 tgataatata tcattgatag catcaagtgt aatgtctaaa aagttagctt caggagctga
    34741 tgcaattgtg ctagatgtaa aaactggtga tggagcattt atgaagacat tagaagagtc
    34801 ttttgagtta gctaaatcca tggttgatat aggtactggt atgaatagag atacaatagg
    34861 gattgtaaca gatatggatg aacctttagg ttttgcagtt ggaaactctt tagaagttat
    34921 agaagcaatt gagacattaa aaggaaatgg acctaaagac cttgttgagc tatgtgaagt
    34981 attaggagca tatatgttgg ttttatcaaa ggtagcgcat gattttgaag atggtgttga
    35041 aaagataaga gaatcaataa aatctggtac tgctatacaa aaactcaaag agtttataga
    35101 gaatcaaggt ggtaataaag atgtagtaga tgattattct ttattcaaaa aatcaaagta
    35161 taaatatcca gttaaatcaa ctaaatcagg ttatataagt aaaattaaag cggaagatgt
    35221 tggtgtatca gctatgattc ttggagcagg tagagaaact aaagatgata ttttagattt
    35281 atcagcaggg ataattttag aaaagaaagt aggagattat gtaaatgaag gtgaagtttt
    35341 agcttacatg tattataatg atgaacaaaa gctttcattg gcaaagagaa gatttattga
    35401 ttcttattct attgtagatt caaaaataga aaaaaataaa ttaatatatg gtattgtaac
    35461 taaagaagat attaaaaagt tttagaaaat tttagaaaag tgattataaa aagcctaaca
    35521 tgtcaaaaat aatgttaggt tttttatttt ttgataagat ataaatgatg gcaaaaatat
    35581 atattttaaa acatttttta caatataaat aagtttattc tacaagatga ttgaaaatta
    35641 aagtatctag ttttataata gaaaatggat aaatatacat aaggagtaga ttatattatg
    35701 aactttaaag aaaatagatt gaaaatagca gtattaataa ttgttatttt gattatctta
    35761 gcaggtatat ttgtgtttat acaaataggt ccttatgata aaaataacaa aaaagatgta
    35821 attattgacg ttccaagtgg agcatctgtt ggaaaaatat ctgacatatt atatgaaaat
    35881 aaattaataa agaatgaact attatttaaa cttttggtaa aggttagcaa taaagctcca
    35941 tctataaaat caggaacata cttacttaat caatcgtatt caaataatga tataataagt
    36001 cttttagttt caggtaaaat atatcaggat ggtataaaag tgactattcc agagggagct
    36061 acatctaaag aaattatagc aatgttagta agtaaaaatt taggagataa agctactttt
    36121 gaaaatttaa taaaaaaacc tcaggaattt tatgataagt ttccatactt gaaggaagat
    36181 ggaataacct ctttagaagg gttcttgtat ccagaaactt attactttaa ttcaaagaaa
    36241 caatcagaag aagatatttt aagcgaaatg ttaaaagtat ttgacagtaa atacacggac
    36301 aagtttaaaa agaaacaaaa agaattaaat atgactcttc aagaagtaat ggaaatggca
    36361 tcaataattg agaaagaagc tgttcttgac aaagacagac ctataatagc aagtgtgttt
    36421 tataatagac ttaaggtagg tatgcctctt caatcagatg ctacaataca atatatattt
    36481 gaagagagaa agaaaattgt aacatatgat gatttaaaaa ttgattcacc atataatagt
    36541 tataaaaata agggattacc tcctacacca atatcaaatc ctggtataga atctattgaa
    36601 gcagcattat atccagataa aacagactat ttatattttg tagctaagat tgatggtgga
    36661 aataattata gtacaaacta tcaagaccat ttaaaatatg ttaaagaata taaagaagca
    36721 agggataaac aatcaaaaga tacaaaagca acaaataaag aaaatacaaa aagataaacc
    36781 atgattataa aaagtctaac agggtaagaa taatgttggg cttttttatt gaaaattgtt
    36841 attcttagat ttataataaa
//
LOCUS       DAEPXF010000017        36596 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain HB27
            SAMN13376389-rid12479423.denovo.018, whole genome shotgun sequence.
ACCESSION   DAEPXF010000017 DAEPXF010000000
VERSION     DAEPXF010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13376389
            Sequence Read Archive: SRR10518432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 36596)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36596)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001069624.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001069624.1
            Genome Coverage       :: 315x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/15/2021 01:54:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,165
            CDSs (total)                      :: 4,076
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 89
            rRNAs                             :: 10, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 10 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 19 of 69
            Pseudo Genes (incomplete)         :: 44 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 10 of 69
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36596
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13376389-rid12479423.denovo.018"
                     /strain="HB27"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="China: Hebei"
                     /collection_date="2017"
     gene            complement(158..2056)
                     /locus_tag="KRL67_000782"
     CDS             complement(158..2056)
                     /locus_tag="KRL67_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CotH kinase family protein"
                     /protein_id="HBH1521687.1"
                     /translation="MKDKKFTLLISIMIIFLCAVVGVYSTSSNKGVDLYSDVYIEKYF
                     NRDKVMEVNIEIDESDLKDMNENAIKEEFKVAKVTVDGDTYGNVGIRTKGNSSLTSVA
                     NSNSDRYSYKINFDKYNTSQSMEGLTQLNLNNCYSDPSYMREFLTYSICEEMGLATPE
                     FAYAKVSINGEYHGLYLAVEGLKESYLENNFGNVTGDLYKSDEGSSLQYKGDDPESYS
                     NLIVESDKKTADWSKVTKLLKSLDTGEDIEKYLDVDSVLKNIAINTALLNLDSYQGSF
                     AHNYYLYEQDGVFSMLPWDFNMSFGGFSGFGGGSQSIAIDEPTTGNLEDRPLISSLLK
                     NETYKTKYHKYLEEIVTKYLDSDYLENMTTKLHDMIASYVKEDPTAFYTYEEFEKNIT
                     SSIEDSSDNKGFGNKEFNNNNSNNSDSNNNSNSENKRSGNQSDEKEVNAELTSSIVKT
                     NTDNETKNKTTNDSENKNNTNKDKSGNDNNQKLKGPMGKGGKSIPGVLEVAEDMSKTI
                     KSQLSGETSSTKQNSGDENSSGIKGTEKFDEDMSGMPEPPEGMDGKMPPGMGNMDKGD
                     MNGKNGNMNMDRNQGNPGGFGNRGGGSMSKTTIYSKLILGGASMIIMSIMLVGVSRVK
                     RRRFIKSK"
     gene            complement(2073..2759)
                     /locus_tag="KRL67_000783"
     CDS             complement(2073..2759)
                     /locus_tag="KRL67_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4956 domain-containing protein"
                     /protein_id="HBH1521688.1"
                     /translation="MILIMTFNDIFKSSFIENVSGFSFVDSALALGSAFLVGLFIYMV
                     YKKTYMGIMYSRPFNVSLVALTMLTTFVILAVTSNVVLSLGMVGALSIVRFRTAIKDP
                     MDLVFLFWSLGSGIVLGAGLIPLAVMGSIIMGLILIFFSNKTISETPYILMVNCNNED
                     SEDIATDKIKKVFTKYQIKSKSVTPEKGIELVFEVRMKDGETSLINDLSQVDGVTNAV
                     LVSYNGDYVA"
     gene            complement(2864..3553)
                     /locus_tag="KRL67_000784"
     CDS             complement(2864..3553)
                     /locus_tag="KRL67_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate polymerase domain-containing
                     protein"
                     /protein_id="HBH1521689.1"
                     /translation="MNKKLNYRFEMKHKITEADVLALKSRLYPTMKKDENASKDGKYL
                     IRSLYFDTPEDKALLDKLNGVATREKFRIRFYNNDCSYIRLEKKIKHYNMTSKLSASL
                     TKKEVINILNNDIEFLKESTNCLLREFYLKLKCERLEAKSIVDYNREAFIYPVGNVRV
                     TIDSDIKTSVNSVDLFNKDLPTVSVIDENMTVLEVKYDEFIPDFIKDLIQINKTTSTA
                     VSKYASSRLYI"
     gene            complement(3886..5748)
                     /locus_tag="KRL67_000785"
     CDS             complement(3886..5748)
                     /locus_tag="KRL67_000785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBH1521690.1"
                     /translation="MSEKRTDKQDIEVLSFEDGMSAEKAKDAKKTTKRLLKYMAKQKF
                     KILIIFISVLISSALTVLAPMVMGKAIDQLFNGIKTAVQTGTKFSVNFSTMGGIVSIL
                     LGLYLISAVFIYIQQFIMSGVAQNLVLSMRKDLSDKLNKLPLKYYDSHKKGETLSIVT
                     NDLEKVADSLQEGLMQLITAVVTVVGSIVMMISISVPLTIVSAITLLVSLGITVVIAR
                     KSQERFSENQKALGELNSNIEEIFTGQIVVKAFSKEKDTIRNFKILNQNLYNASSRAQ
                     FSSYAISPIIRFINLIGYVIIAVVGGIFASTGAMTLGSIQAFIQYVNQASEPTTEISY
                     IVNMLQAAIASAERVFTVMDEVEEIKDEESSKVISMPKGKVQFEHVKFGYSDDFILMK
                     DININLNAGDKIAIVGPTGAGKTTLVNLLMRFYEIQGGRITIDGVNIKDLKRGELRTM
                     FGMVLQDTWLFNGSIKENIAYSKLDATMDEIVRAAKSARVDHFIRTLPQGYDTILTED
                     ASNLSQGQKQLLTIARAILSDPSVLILDEATSSVDTRTEVEIQKAMNNLMKGRTSFVI
                     AHRLSTIRDADLILVMKEGTIIEQGRHKELIEKKGFYEELYNSQFSSEYDEDVV"
     gene            complement(5786..7513)
                     /locus_tag="KRL67_000786"
     CDS             complement(5786..7513)
                     /locus_tag="KRL67_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBH1521691.1"
                     /translation="MKKLIHFLKPYRVLIVIVLIFTFLQTLGTLYIPTLTANIVNNGV
                     VKGDIDYIVKTGLMMMIVAGITALSAVLVCKVSANLSSGFCRDIREAVFVKSQDLSIN
                     DFNNIGTASMITRSTSDITLIGQSVFMFIQLVLPAPIITVSGLFLAYSIDKVMTVIIV
                     VVMFLFMLSAFLVGKKLIKLFKMMQVKMDNMNRVLREVVTGVRVIRAFNRSHFEKKRF
                     DRTAIDYSETAISINKIFAVLMPIVMLIMNLGIVSIIWFGGMRVSNGNMEIGHIMALV
                     EYCILILFYLIMGVMVFMYIPRAGACADRVNQILDIEPEIVDGNGREDVVSERGHLVF
                     KNVTFSYAQSEEPVLNNITFEAKSGEVTAIIGSTGSGKSTIANIIPRFFEIQSGEISI
                     NGQDIKKIPQKELRDKIGFVPQKAFLFSGTIEENIRYGKEDASIEEVKHAASIAQADE
                     FISDMEDKYDSFVAQGGNNLSGGQKQRISIARALVRKPEVYVFDDSFSALDFKTDKRL
                     RKALKNEIKDSSAIIIAQRISTIMDANQIIVLNDGKIVGIGKHKDLLENCEVYKQIAD
                     SQLSKEELA"
     gene            7586..8416
                     /locus_tag="KRL67_000787"
     CDS             7586..8416
                     /locus_tag="KRL67_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HBH1521692.1"
                     /translation="MSQVSKNYFTTGEFAKICGINKKTLFHYDDIGLFSPELKKENGY
                     RYYSYHQLSIFGIISSLREVKMPLKEIKTYIDKRTPNLLIELLEKKTIDIKNEIEKLN
                     NIQALMESTISFTKNACNIDANTIILKEHEEEYLVKTPIIYKEQFLGDEEENFLYECI
                     NFMDNYELSDYGTIGSIIKGEDIINKDFESYSYLFTKVNKEYKKYPVSIKAKGLYVTA
                     YHKGSYETIYKTYEKLLNFFNQNNLCMGDFVYEEYLLYDISVRDSNEYLTQISAEVKM
                     "
     gene            8433..9410
                     /locus_tag="KRL67_000788"
     CDS             8433..9410
                     /locus_tag="KRL67_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HBH1521693.1"
                     /translation="MIQSEISDRSNYKKINTLEVVIFFVVTYGISLLFGIPLYFKEYM
                     NPQIMVTFMTILPASGVSIAKIYKHKKSDEHIILHCVIIADFLLSLFLVTLKAFGAID
                     DETVILGMGILVLFTGIITICYACLEGKELYPFKNVNKSIICIFIFIIIINLSKCIML
                     GWKDINFINLIMNIFLIFPYIANQSILFFGEEYGWRGFLQEKMQTKFGKRMGVILLGI
                     IWELWHIPLWFTIYHVDGLGILLRLFSVISLSIFFGYVYMKTKNVWSCVVIHLINNTV
                     LLDTNLNNAFLNFNTSSLNSSVILGCLIFSVLLSLFIFSKEYKKEQLIY"
     gene            complement(9630..11243)
                     /locus_tag="KRL67_000789"
     CDS             complement(9630..11243)
                     /locus_tag="KRL67_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HBH1521694.1"
                     /translation="MKEQLIFNITNLVTVVFEAFVIHMFLSDFLGQKEGYIKIVRYAK
                     LGFIICLGFCNLITLNPKITMPLIFILIFSTSFLYKGNLKTRLFTTVLLSIFFILSEI
                     VVTSIFVLFVKEGFEIMFENNSIRVLATILSKIVFLLTCKIICLFKKDVHLDMPIKYW
                     LPLFLIPIFSLFLSVSIFDVSKFFSLESLKFLSLISSVGILYINFIVFYLFKFIIDKT
                     KLSMKYEILEKEIIHKEELRLSNECYKIIVEQTDSVVFEWNIKENKSFVSQVWTEKFG
                     YNNACKNIFKEIKDKDLVHSEDKAIFESFLESIKKKNIHSQAVYRLKKSNGEYIWCRT
                     SITSIYNDENELLRVVGVIVDVDSDIKKFEELRTRAESDSLTNIYNKGTFEKLVEETI
                     VMNTGDKKDALFIIDLDDFKEINDNFGHPFGDFVLKTFADKIQASFGSKDLVGRIGGD
                     EFVVYMQDYVTEVNLHKKAKELNRVLSDNYTDLSFSFDASVSIGIARYPQDGTSFFEL
                     FKNADRALYSIKASGKNSYCLFEEELYVQ"
     gene            complement(11448..12935)
                     /locus_tag="KRL67_000790"
     CDS             complement(11448..12935)
                     /locus_tag="KRL67_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBH1521695.1"
                     /translation="MKNGNDKNRIGKIKSKFKLKFSKKNISIKTRILLSILCLIIIVF
                     MIILVSFNVFLDTYIKTTANEELTKSTETVEHMDSNFRPIKPPQYDGKLPPKVLSNFM
                     RNVQDKVRMAETQSDADAMVVDSKYNLIFPTREDDFLKNVDEMELIRECIQNEKLDLN
                     SDENTRISTRNKDYYISTVKITSIYGEQDKYLILFIDISKTLNLAQKINIVLISVMCL
                     AGILAIFTAVILSEKIAKPIKELCEFAKKIGQGDFKRNYFDFSDKELVELSTVMNKSA
                     EYLDKYDNEQKIFFQNASHELRTPLMSIKGYAEAIKYNVIDSKHASDIILEESDRLSD
                     MVEDLLYISKIDNITKDFQLVECDLREVLSNCGARQNVRAINKGIKFIYEFDEEMVLF
                     ECDEKNISKAFMNLIENALRYAKSEIKIVCKYNQKNIVVIIEDDGIGIKKEDLPHVFE
                     RFYKGVGGNHGIGLSIVKSIVNKHGGRIYVENGKKGAKFTILFKL"
     gene            complement(12937..13641)
                     /locus_tag="KRL67_000791"
     CDS             complement(12937..13641)
                     /locus_tag="KRL67_000791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBH1521696.1"
                     /translation="MSKLIYIADDEDNIRNLVKTFLKNEGHDVMDFKTGDELLEQFNI
                     KECDLVILDIMMPGSSGFEVCTKLREKSTVPIIMLTARDTDIDYITGITLGSDDYFTK
                     PFSPMSLVMRVKSIFRRIEFEKKQNYDKYSNSIDMELKFGDVIINKKNKIVTSKNVNI
                     DLTPNEYNLLTYLFENIDRAVSRDELLNKVWGYDIEVETRAADDTVKRLRKKILDTNI
                     LIETVWGFGFRLKEKS"
     gene            14198..14617
                     /locus_tag="KRL67_000792"
     CDS             14198..14617
                     /locus_tag="KRL67_000792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH1521697.1"
                     /translation="MKKALRYLLFLIILLLMYFLAVKLNYDFYTQFHTGYMQDFKRYI
                     FINLISSGGIGLLLGTELLIREYKNDGSWYIDIPRLLLLSFPSFLLSLMPVFFFMFPV
                     GNIPIIGNFIMLDRIPLNIIIFNILFGYFLITSFRKK"
     gene            complement(14749..15498)
                     /gene="nagB"
                     /locus_tag="KRL67_000793"
     CDS             complement(14749..15498)
                     /gene="nagB"
                     /locus_tag="KRL67_000793"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012425552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HBH1521698.1"
                     /translation="MRVLVCKDYDGMSKKAAEMIAAQIVLKPNSILGLATGSTPVGMY
                     RDLVKKYNDNIVDFSDVMSFNLDEYYKLPISNDQSYDYFMKENLFNHVNIKPENTHLP
                     NGMADDIEKECMNYEASIDAAGGIDVQVLGIGRNAHIGFNEPDNKFAKRTHVVELTES
                     TIEANARFFKSREDVPKKAVSMGIGSILKSKKILLLASGEEKADAVYNTVYGDITPEV
                     PGSILQLHKDTIVIIDEAAASKLNPKDYKLV"
     gene            complement(15596..16729)
                     /gene="nagA"
                     /locus_tag="KRL67_000794"
     CDS             complement(15596..16729)
                     /gene="nagA"
                     /locus_tag="KRL67_000794"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HBH1521699.1"
                     /translation="MKCLINGKIILKNQILENKVLVFDEKIIDIADSVPKDCEVIDAD
                     GKYISPGLIDIHIHGNMGKDTMDSTDESIETISKSIMRHGVTSFLPTTMTMDKEHVYD
                     ALEVIKKAQNRKLEGAQVLGAHLEGPFINENYKGAQNEKFIINSKYEFIKEYKDVIKV
                     ITYAPEKDIEFDFTREIKRCTDIVLSIGHSNANYEQAKEAINLGVTNVTHMFNAMTGL
                     NHRDPGVVGAALTTNVYSELIADTIHINKDLFQFILNNKGKERLILITDSIEAGGLED
                     GNYSLGGQKVIVKGNEARLENGALAGSVLSLNKMVFNFLDNTNLKVNEAINLASLNPA
                     TSLGINDKKGSLEIGKDADIAVFDENLDCKMTICLGEVVYKNI"
     gene            complement(16739..17473)
                     /locus_tag="KRL67_000795"
     CDS             complement(16739..17473)
                     /locus_tag="KRL67_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBH1521700.1"
                     /translation="MKIVNKSSNIPLHTQLSSIIREMIETGELKEGDAIMPERELCNI
                     QNVSRMTVNKTIVGLVTEGLLYRVQGKGTFVAKQKKKYQFSNVKGFTDVMKEKGVNIK
                     TDILSFEMELPDDLVKRKLGISDNTTNIYKIVRLRYTDGEPFGLEIVYLSEEMCKGLT
                     KGILDNSSLYRVLNEKYGYKIQKAEQVMEPVILSDEESKLLETDEGALALKLHRNSYN
                     REGSPIEYTISIFRTDKYQYEIVLSE"
     gene            17768..18610
                     /locus_tag="KRL67_000796"
     CDS             17768..18610
                     /locus_tag="KRL67_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HBH1521701.1"
                     /translation="MINIGDFNKLTVKRKTEFGYFLDGQTNNTKDDILLHNRLIGKNE
                     INIGDEVNAFIFKDSDDRTAATLIPPLAKVGDVARLKVVDNTDIGTFIDMGLPKDILV
                     PFKAKTYPLFRDEKYLFYIYLDKSKRIAATTDIDSYLLTDHTYNVGDIVTGVVYGFQT
                     NNSAMICVDNKYAGVILHNEYFTELKTGDVLENLHVIKIYEDGKLGLSPRGNRKDELD
                     TLENKILSYLEGSDGYMRFNDKSDPKDISILFNSSKKNFKRALGVLMKKGLIYQDEEG
                     TYLK"
     gene            complement(18808..21141)
                     /locus_tag="KRL67_000797"
     CDS             complement(18808..21141)
                     /locus_tag="KRL67_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HBH1521702.1"
                     /translation="MRSYLELVPQYAKVHKKKNRTTILCIVIAVCLVTAIFGLADMAI
                     QCQKVQFIKDDGNWHIVLKNLNEEAISEISSRVDIAHYGRGQVIDNGEFKSKEILIQG
                     LDEDLAKQLGLEIIKGRYPVVENETLIDVDAVKSFNLKVGDFIKIKLADGRNKNYKIV
                     GEYSSLSSLRASDKHGLMLSILGTQAIKKSETYDYYYITFKDKVNIRKATSDIKNTFN
                     LSDKQIGKNEKLLSIIGQGDSDYSKPLYLTAGGLFILVLIASIIMIYNSFNMGVMERI
                     KFFGLLRCLGASKLQVKKFVVLESLILSVKAIPLGLLLGCIVTIISSIFLKYVNTELF
                     GSMPILKVSFIGIVFGIIVGFLTVILSAIVPAKKASGVSPLSAIRGNLMFKEILTSGK
                     ASKIANAGSSKIDVSMGINHAFSRKKSFILMTSSFAISIVLFLGFSVFIDFMYQALKP
                     IKPEATHISIVDADFKSVLTKDDINEISNIKGIKKVYSRMMTDVEATYNNKEATSQLI
                     SYEKNQFDWAKKYLVKGEINEDKLESENIGLVEEGHGFNIGDKIYIKNGNSKKEIEVA
                     GILSSISFDVKDKYIGNIITSENTFTSITDIKDYIIIDTQVDEDAPEDLIQNIRNVLD
                     DNLEVRDKRQGNLEAKNSFYTMAVFVYGFVFIIAIISMFNIINSMNISVTSRINYYGI
                     MRAIGMSNKQLRKMVIVESSTYAVSGCLLGSVFGLILHRYIFVSLVTLKFHIDWQIPF
                     DLLFIIVVTMIIITLLAVRKPIKKICELDIIEVVNAQ"
     gene            complement(21138..21821)
                     /locus_tag="KRL67_000798"
     CDS             complement(21138..21821)
                     /locus_tag="KRL67_000798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBH1521703.1"
                     /translation="MDILVVNNLCKTYGNGYTKVEALKDVSFTVSKGEFVTVVGESGS
                     GKSTLLNLLGGLDTPTSGKIFVDGKEICSMKEEKQTIFRRRNIGFIFQSYNLIPELTV
                     EQNIIFPLLLDYKKPDTIYLDEVLTILGLKDRKSHLPNQLSGGQQQRVAIGRALITKP
                     SIILADEPTGNLDSKNSSDVISLLKVTAEKYNQTIIMITHNTNIASTADRILQVSDGM
                     LKDLGVNRE"
     gene            complement(21893..23134)
                     /locus_tag="KRL67_000799"
     CDS             complement(21893..23134)
                     /locus_tag="KRL67_000799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBH1521704.1"
                     /translation="MKIFSNKDIKMFFLILISILVMFIIVGQIVLISITNNYKKTLIE
                     HDYNIAGYLYSIGVDKSKIPSAFVAHEDKYILKGKEILDTAGYGFDIDVSFTPSVKLF
                     QSKYIKSMSIYLLVFFTMIIFILYMYFFRQQSKIEEASYKIDSFMNGNINERLNTYEE
                     GSLSKLFSSIDVMSTSLNTHILKEKQNREFLKNTISDISHQLKTPLTALIMYNQILQE
                     ESHNSDVVEAFVQKSQSELERIETLIKNLLKITKIDSKTIVLNKNNVNLKNLIVKILY
                     SFETRAEKEGKVISINGSENTLLFCDYEWMSEALSNLIKNALDNTKENDKITIEWFET
                     TITTIVSVKDTGNGIHVEDIHHIFKRFYRSKFSKDNQGLGLGLPLVKSIVEQHNGTIS
                     VESKLSQGSTFTISFLKLTNM"
     gene            complement(23145..23828)
                     /locus_tag="KRL67_000800"
     CDS             complement(23145..23828)
                     /locus_tag="KRL67_000800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBH1521705.1"
                     /translation="MSSILLVEDDLSLIYGLEFSIQKSGFNIDIARTVKEAMQMYEEK
                     NYDLLLLDVSLPDGNGFQICEKVREVSNIPIIFLTASDEEVNVVMGLDMGGDDYITKP
                     FKLNELISRIKALLRRYNVASNVNELSSNNITIKLLENRVFKGEFEVELTTAEYKLLC
                     LFMKNKNIVLTRKNILDKLWDGNGSFIDDNTLSVYVRRLRNKIEDNPENPKFLLTVRR
                     MGYKWNVIK"
     gene            complement(24352..24615)
                     /locus_tag="KRL67_000801"
     CDS             complement(24352..24615)
                     /locus_tag="KRL67_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HBH1521706.1"
                     /translation="MEETKFCQCCAMPMGQTDELYGTNKDGSKSTDYCSYCFKDGEFT
                     ADISMEEMIEVCIPHMLQSNKDMTEDEARTMMNNFFPTLKRWN"
     gene            complement(24788..25969)
                     /locus_tag="KRL67_000802"
     CDS             complement(24788..25969)
                     /locus_tag="KRL67_000802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent malic enzyme"
                     /protein_id="HBH1521707.1"
                     /translation="MSKDYAKLALEMHEINKGKVSVESKVEVKTKDDLSTAYTPGVAE
                     PCLKIHENQDDVYRYTSKGNLVAVVSDGSAVLGLGNIGAEASIPVMEGKAILFKQFAD
                     VDAFPICLKTNDVDEIVKSVELMEPVFGGINLEDISSPRCFEIEERLKKSLSIPVFHD
                     DQHGTAIIVAAAIINSIKLIENKKIEDLEIVINGAGAAGIAIAKILLNMNVKNIILCD
                     RSGALEASIENLNYVQKEMLKVTNIRNEKGPLKDIIKGKDVFIGVSGPGAVTKEMVES
                     MSEKPIILAMANPTPEIMPEEAKLGGAFIMGTGRSDFPNQVNNVLAFPGIFRGALDVK
                     AKEINEEMKIAAAYAIANTISDEEICPEYILPDVFNKNVVKNVAKAVKEAAIKTKVNR
                     I"
     gene            complement(26035..26583)
                     /locus_tag="KRL67_000803"
     CDS             complement(26035..26583)
                     /locus_tag="KRL67_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S-containing hydro-lyase"
                     /protein_id="HBH1521708.1"
                     /translation="MIKITTPVNEIDIAKLNCGDTISLSGILYTARDAAHKRLIDCIN
                     KGEELPFDVYGQGIYYVGPTPTKPGEVIGAAGPTTSYRMDDLTIPLLERGLRLMIGKG
                     KRSDEVIEGMQKYGAVYLAAIGGAGAYISNSIKSCEIIAYEDLGAEAIRKIVVEDLKL
                     TVAIDSYGNNIYEQGRAIYECK"
     gene            complement(26596..27435)
                     /locus_tag="KRL67_000804"
     CDS             complement(26596..27435)
                     /locus_tag="KRL67_000804"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase"
                     /protein_id="HBH1521709.1"
                     /translation="MRKIKSEQIVEQVKKICIEASLYLGEDVLSCIKEKAKSEKSEVG
                     KNILNILVENAEIAKEKNIPICQDTGMAVFFVEIGQEVLIEGDTLTDAINEGVRQGYE
                     EGYLRKSVVSPINRVNTKDNTPAVIHYDMVKGDKIKIEFAAKGFGSENMSKMKMLKPS
                     DGLEGIKKFIIDTVSEAGPNPCPPMVIGVGIGGTVDKCAQIAKKALFRELGEFNKDEN
                     IAKLESELLTAINKLGIGPQGLGGTTTALGLNIETFPTHIAGLPVVVNINCHASRHKK
                     VVI"
     gene            complement(27632..28105)
                     /gene="ybaK"
                     /locus_tag="KRL67_000805"
     CDS             complement(27632..28105)
                     /gene="ybaK"
                     /locus_tag="KRL67_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro) deacylase"
                     /protein_id="HBH1521710.1"
                     /translation="MVKTNAMRILDSNKIDYKVMSYEVKSEHVDGVEVAHDIGRDVNE
                     VYKTLVTQGVSKNIYVYVIPVHENLDLKKAAKVAKEKSVEMIHVKDINKLTGYIRGGC
                     SPIGMKKLYKTFVNESAKNLDTIIVSAGKIGYQIELSPFDLQRLIKVEFVDVIKK"
     gene            complement(28228..28926)
                     /locus_tag="KRL67_000806"
     CDS             complement(28228..28926)
                     /locus_tag="KRL67_000806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBH1521711.1"
                     /translation="MEKIRLSIENINKRYDSHVIFRDFNIDFYVNEVNCILGKSGCGK
                     TTLLNIISGIIKNDTNNLNIKENLNKVGNKLEASYIFQDDRLIDWLTVEENIKIVVNK
                     YYNKTQLNKICDEYLELVGISDYKKFYPQMLSGGLRQRVNIARAFIYPSKNIIMDEPF
                     KSIDAKNTQLIMDNFRNILRKEKRTVLFVTHNIEEALFLADRIFILGDSPIRIKKILK
                     NSKELEKNEVLKLI"
     gene            complement(28914..29699)
                     /locus_tag="KRL67_000807"
     CDS             complement(28914..29699)
                     /locus_tag="KRL67_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HBH1521712.1"
                     /translation="MKQYTLLRNKKNKEQFIDKMEVTLSCVILLFLWQIIALKIDNDI
                     FLPTVGQVFNSIKEIMLNSSFYIDILYSMGRCFFSFLLAMIFAIILSIVSYLNRFFRN
                     LLKPINALTRSIPTMILVVLALIWFEKDSTPFIVGFAIVFPILYDNVLGAVLNIDKNL
                     LEMANVYKIRFIDKVLKIYLPAIKFQIISILVSTFSLSLKVAIAGEVYSQPTYGIGTM
                     IQTEKINFNTSGIFAWIIIVLLISAILQIAQKFLARRAFLWKR"
     gene            complement(29671..30666)
                     /locus_tag="KRL67_000808"
     CDS             complement(29671..30666)
                     /locus_tag="KRL67_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBH1521713.1"
                     /translation="MKKYISIILLVVVTMVVLVGCSPGKDNSKNKELSVVKQVKVAVP
                     DGLPAVAIAKLANENPEIKDGYETVYSIEKTPEAISTRVMKKDADIAIVPSNMAAIAY
                     NKTSSYNIVGTVGMGSLYLVSTENIKDYSDLVGKEVGCTGKGLTPDITIKSLLLQKDI
                     NYSDIKFNYVNSASELVPLLATGKVKTGIVPEPALSALMSKNPDIKIIKSLNDSWKEV
                     SGSKDGYPQSTLIVKSEFLRDNKDFVDSFVGQLSNSIDWANKNPGELGAYSEKIKITT
                     ESKIIGKSLERANLKYIPVKDMIKDYKNYYEKLANFDDKTLGGKVPDEAIYFVEK"
     gene            complement(30772..32706)
                     /locus_tag="KRL67_000809"
     CDS             complement(30772..32706)
                     /locus_tag="KRL67_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgiQ family radical SAM protein"
                     /protein_id="HBH1521714.1"
                     /translation="MENKFLPISKQDMIDRGWEELDFVLVTGDAYVDHHSFGTAIISR
                     VLENAGYKVGIIAQPDWRTTDDFMKLGKPRLAFLVNAGNMDSMVNHYSVSKKHRDKDM
                     YSPGGKMGYRPDRATIVYCNKIREAYSDVAIVIGGIEASLRRFAHYDYWSDKVRKSML
                     IDSGADLLVYGMSEKQIVEVANALNDGYDPKYIRHIDGTCYISDTLEEIYDKYILIPS
                     YKEICEDKMKYVEAFKIQYDEQDPFRGNIIVQPHGSKYLVQNKPEKPLSREELDEVYG
                     LPYQKTYHPVYEKFGGIPAIEEVKFSIVSSRGCFGSCSFCAITFHQGRAVQSRSEKSI
                     IDEAIGITNLDDFKGYIHDVGGPTANFRRPACKKQITKGACKNRQCLSPSPCKNLDAD
                     HSEYLHLLRAVRKLPKIKKVFVRSGIRYDYVMADKNNKFLRELIEHHVSGQLKVAPEH
                     ISEEVLKYMQKPAGKTYDKFRQKFFGINEELGKKQYLIPYLMSSHPGSTLNSAIELAE
                     YLRDTHYQPEQVQDFYPTPGTLSTTMFYTGIDPLTMKPVYVPKSKRDKAMQRALLQYR
                     APRNYDLVYSALVEAGREDLIGFGHRCLIKPKNEKPYFNRNNSKKNVSKGTNKNKKTN
                     TNNRSNKNQQKSSTKKKKKH"
     gene            complement(32891..36439)
                     /locus_tag="KRL67_000810"
     CDS             complement(32891..36439)
                     /locus_tag="KRL67_000810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HBH1521715.1"
                     /translation="MMNRLKPRILGISILFIIIIILTCGAVFKFKFKLDNKLKETTHT
                     YLSEFTEHNIINLKTRLSGQFDMLESIANFIGNLNNINDEMVIDLMDSDVKRSSLIRM
                     TISTLDGECYSNDGITHNVQDRDYFKKAKNGEKNISEPLKSIVNNKEIIVLSTPVYKS
                     GKIVGVLSGIYDSKKLNELLGLSAFDEKADVFVAKKNGDIIAQKTNKNSKTISCIFQL
                     FDKDNCDEVDCLKTIKENMKNDKSGYFNYNLDNEKFMLNYQPLGVNDWYVFSVVPNKI
                     VSIQSDNISYDVYIFALEILLVILIFIIYIACIINNNTKIIINEKEKFKVLTDNILGG
                     VKTCLKDEYMTMTYVSQGFLELTGYSCEDLNKIFKNRFKDMIYKDDLDKFNCIILNQL
                     AKQDKIEVEYRIVRKDGSIIWILERSKSINDNKGSECIQGVLTDITELKKIQEELDSK
                     RFEIQAINDSIVGGIVITEINDDFDSIYLNEGYLNMIGYTRKQLKDELNNKLIKLIFK
                     DDRTELINSINKQLKLSDTFASEQRVEKRDGTIIWILLKGRKIIYDNGKSVLCCVIVD
                     ITSAKLLEQELKKSEERHEIILKQTQNIIIEWDIEKDKMNISPNWYNKFKYDPITENA
                     TKNIIKSVDIYHDDMDKFISLIKLTSQGSEYEECEIRLRTRDTIYAWFSIKATTLFDN
                     NGKPYKVVGSLTDIDESKREAEKLKERAEKDPLTDLYNKKASQSLIEEYITLDKKRKG
                     ALMIIDIDDFKGINDNLGHLYGDAVLSEVANDLTNLFRATDIVGRIGGDEFIVFIKDI
                     SKTRDIINKAEELVKTFERSFLGKGRNYKISLSVGIAKFPQDGMDYMTLFRNADMALY
                     SAKEKGKNCYTLYDKKIDKVQYITNTRSAGDSDNVEMSFKEHITNYIFDILYESSDIE
                     VAVNLILSIVGKHFNVSRMYIFENSDDNRVCKNTFEWCNEGITSEINNLKNICYKDLG
                     NYLLNFNADGLFYCNDIENLGSDVKKVLKPQGIRSILQCKILEGNEFRGFIGFDECSY
                     NRMWTNDEVNTLTFISKTLSMFLLKMRIQSKLEESLTIQKSVMDNMDIWAYVVDRNTY
                     ELLFINKKMLESAPNTHVGDLCYKAIWNNQDKPCKKCPMIYLKDGDDTYKSFMRNKKF
                     EVQANITTTTLKWFDGKDACLIFGSEVTSIEAEENT"
BASE COUNT        11964 a         6312 c         3846 g        14474 t
ORIGIN      
        1 ttttattact ttattatgta ttattaatat gctgtattta atttttatca atttattacc
       61 ttatattatt aatcaaacac attattttct aaaaaaatac tgctaagtaa atatttagac
      121 ttaacagtat taaaattatt tatattcaat tttaatctta ttttgacttt ataaatcttc
      181 ttctctttac ccttgataca cctactaaca taatcgacat tattatcatt gaagctccac
      241 ctaaaattaa tttggagtat attgttgttt tactcataga gcctcctcct ctattgccaa
      301 aacctccagg attaccttga tttctatcca tattcatatt gccattttta ccattcatat
      361 ctcccttatc catgttgccc attcctggtg gcattttacc atccattccc tcaggtggtt
      421 ctggcatacc actcatatcc tcatcaaact tttcagtacc tttaattcca cttgaatttt
      481 catcaccaga gttttgcttt gtcgaagaag tttctccact caattgagat tttatagttt
      541 tactcatatc ttctgcaact tccaaaaccc ctggtattga cttacctcct ttacccatag
      601 gaccttttaa cttttgatta ttatcatttc cacttttatc tttatttgta ttattcttat
      661 tttcactatc atttgtagtt ttatttttag tttcattatc tgtattagtt ttgactatac
      721 ttgatgttaa ttcagcatta acttcttttt catcactttg atttccagaa cgcttatttt
      781 cactattaga attattatta gaatcactgt tattagagtt attgttgtta aactctttat
      841 taccaaatcc cttattatca ctagaatctt caattgaaga tgttatattt ttttcaaatt
      901 cttcataagt ataaaatgct gttgggtctt cttttacata tgatgctatc atgtcatgca
      961 attttgttgt catattctct aagtagtctg aatctaggta ttttgttact atctcttcta
     1021 gatatttatg gtattttgtt ttgtatgtct cattttttaa taacgaggat atgagaggtc
     1081 tgtcttctaa attacctgtc gtaggttcat caattgctat agattgacta cctccaccaa
     1141 aaccactaaa tccaccaaat gacatattaa aatcccatgg taacatagaa aatactccat
     1201 cttgctcata taaataatag ttatgggcaa aactcccttg atagctatca aggtttaata
     1261 aagctgtatt tattgctata tttttaagaa cagaatctac atcaagatat ttttcaatat
     1321 cttcacctgt atccaaagat tttaataatt ttgtgacttt agaccaatca gcggtctttt
     1381 tatcactttc aacgattaaa tttgagtaac tttctgggtc atctccttta tattgcaacg
     1441 agcttccttc atctgattta tataagtctc cagttacatt accaaaatta ttttcaagat
     1501 aagattcttt taatccttct actgccaaat acaaaccatg atattcgcca tttatagaga
     1561 ctttagcata tgcaaattct ggagtcgcta atcccatttc ctcacaaata ctatatgtta
     1621 aaaactctct catataagat gggtcagagt agcagttatt aagatttaat tgagtaagcc
     1681 cttccatact ttgactagta ttatacttgt caaaattaat cttatagctg tatctatcac
     1741 tattactatt tgctacagat gtaagacttg aatttccttt agttcttata cctacatttc
     1801 catatgtatc tccatctaca gttacttttg caaccttaaa ttcttctttt atagcatttt
     1861 cattcatatc cttcaagtca ctttcatcta tctctatatt aacttccata accttgtctc
     1921 tgttaaaata tttttcaata tatacatcac tgtataaatc aacacctttg ttgctagatg
     1981 tactataaac tccaactaca gcacataaaa atataatcat aatcgagata agaagggtaa
     2041 attttttatc tttcataaaa gattactcct ctctatgcta catagtcacc attataactt
     2101 acaagaactg cattagtaac tccatctact tgactcaagt catttataag acttgtctct
     2161 ccatctttca ttcttacttc aaacactaat tctattcctt tttctggtgt aacacttttt
     2221 gattttattt gatatttagt aaatactttt ttaattttat cagttgctat atcttctgaa
     2281 tcttcattat tacaatttac catcaatata tatggagttt cagatattgt tttgtttgaa
     2341 aagaatatta gaatcaatcc cataattatt gaacccatta cagctaaggg aatcaaacct
     2401 gctcctaaaa ctatacctga acccaatgac caaaatagaa atactaaatc cattgggtct
     2461 ttaattgctg ttctaaatct tactatagat aaagcaccaa ccatacctaa agataaaact
     2521 acatttgaag taaccgccaa tattacaaaa gttgtaagca ttgtaagtgc tacaagcgac
     2581 acattgaatg gtcgagaata cataattccc atataagtct ttttatagac catatatata
     2641 aataaaccta ctaaaaaagc tgaacctaat gctaaagcac tgtccacaaa tgaaaaacct
     2701 gatacgtttt ctataaaact tgatttaaaa atatcattaa atgtcataat taaaatcatc
     2761 cttcctattt ttaacttatt tattttttat tttatttatt tttaatttat ttgtttttta
     2821 accttattta tgtataactt tatataaacc acagccttta ttcttaaata tataatctac
     2881 ttgaagcata ctttgaaact gctgtagaag tagtcttatt aatctgaatt aaatccttta
     2941 taaaatctgg tataaattca tcgtatttaa cctccaatac agtcatattc tcatcaatta
     3001 ccgaaacagt tggcaaatct ttattaaata aatctaccga atttacagaa gttttaatat
     3061 cactatcgat tgtaactctt acatttccaa ctggataaat aaaagcttct ctattgtaat
     3121 caactatgct ttttgcctct aatctttcac attttaattt caaataaaat tctcttaata
     3181 aacaatttgt cgactctttt agaaattcaa tatcattgtt tagtatattt attacttctt
     3241 ttttggttaa actagctgat agctttgatg tcatgttgta atgtttgatt tttttctcta
     3301 gtcttatata agagcagtca ttattataaa atcttatcct aaacttttct cttgttgcaa
     3361 cgccatttaa tttatccaaa agtgctttat cttcaggagt atcaaaatat aaactcctaa
     3421 taagatattt tccatcttta gatgcatttt catctttctt cattgttgga tagagtcttg
     3481 attttaaagc tagcacatct gcttcagtta ttttgtgctt catttcaaat ctataattca
     3541 attttttatt cactatttat atcacctctc aatttattta actctatttg atttatttta
     3601 tatttagagt atactttaca ctactttgtt aaatctccgt aaaataagtg catctttatg
     3661 gcatatatgt ggcaaacaat atttacttca ataataataa gggttatttt acttttatat
     3721 tgataaatac attcttcaat ctaatttaga aaggaaattt tatcttattt tgacaaataa
     3781 aaaaggttat atgaaataga aaaatactct tattccatat aaccttatat gttactatat
     3841 ttaacttaat atttatcagt catttagtta cctaaaactt gataatcata caacatcttc
     3901 atcatattca cttgaaaatt gactgttata aagttcttca tagaatcctt tcttttcaat
     3961 aagttcctta tgtcttcctt gttcaataat agtgccttct ttcattacta atattaaatc
     4021 agcatctcga atagttgata atctatgtgc aataacaaaa ctagttcttc ccttcatcaa
     4081 gttattcatt gccttttgaa tttctacctc tgttctagta tctacactag atgttgcttc
     4141 atcaagaatc aatactgatg ggtctgaaag tattgccctg gcaattgtta gaagttgctt
     4201 ttgaccttga gatagattag aagcatcctc agtcaatata gtgtcatacc cttgtggtaa
     4261 agtccttata aaatggtcta ctcttgctga ttttgctgct cttacaattt catccattgt
     4321 ggcatctaac ttactatatg caatattttc ttttatactt ccattaaaca gccatgtatc
     4381 ttgaagaacc ataccaaaca tagttctaag ttctccacgc tttaaatctt ttatattaac
     4441 accatcaata gtaattcttc caccttgtat ttcataaaat ctcataagta aatttacaag
     4501 agttgtcttt ccagctcctg tgggtccaac aattgcaatc ttatctcctg catttagatt
     4561 gatattaata tctttcataa gaataaaatc atcactatat ccaaatttaa catgctcaaa
     4621 ttgtacttta cctttaggca tgctaataac ctttgaagat tcttcatcct ttatttcttc
     4681 tacctcgtcc ataactgtaa aaactctctc tgcagatgct attgctgctt gaagcatatt
     4741 tacaatataa gatatttctg tagtaggctc tgatgcttga tttacatatt gaataaacgc
     4801 ttgtatacta ccaagagtca ttgctccagt tgatgcaaat ataccaccta caactgctat
     4861 aataacatag ccaattaggt ttataaatct aataattgga gatattgcat atgacgaaaa
     4921 ctgtgctcta ctacttgcat tataaagatt ttgatttaat attttaaagt ttcttatagt
     4981 atccttttct ttactaaatg ctttaaccac tatttgacca gtaaatatct cttctatatt
     5041 gctatttaac tctcctagtg ccttttgatt ttcagagaat ctttcttgtg attttcttgc
     5101 aattacaaca gtaataccca atgaaacaag taatgttata gctgatacta ttgtaagtgg
     5161 tacgcttata cttatcatca ttacaataga accaactact gtaactactg ctgtaataag
     5221 ttgcattaat ccttcttgta aactatctgc tactttttct aaatcatttg ttactatact
     5281 aagtgtttcc ccctttttat gagaatcata gtacttaaga ggaagtttat tgagtttatc
     5341 acttaaatct tttctcatgc taagaactag attttgtgca actcctgaca taatgaactg
     5401 ttgaatatat ataaatactg cactaattaa gtacaaccct agaagtattg atactatacc
     5461 tcccatagta ctaaaattca cactgaattt tgtacctgtt tggactgcag tttttattcc
     5521 attaaatagt tggtcaatag cttttcccat aaccataggc gccaaaactg taagtgctga
     5581 acttatcaat actgaaataa aaataattaa tattttaaac ttttgttttg ccatatattt
     5641 taataatctc ttagtagttt tttttgcatc cttagctttt tctgctgaca taccatcttc
     5701 aaaactaagt acctctatat cttgtttatc tgttcttttt tcactcatat tacatacctc
     5761 caaatacaca gattaattct acatcctatg caagctcttc tttactaagt tgtgaatctg
     5821 caatttgctt ataaacctca caattttcaa gtaaatcttt atgttttcca ataccgacaa
     5881 tttttccatc attcaaaact ataatttgat tagcatccat gattgtgctt atacgctgtg
     5941 ctataattat agctgatgaa tctttgattt cattcttcaa cgcttttcta agtcttttat
     6001 ctgtcttaaa atctagtgca gaaaaactat catcaaagac atatacttca ggctttctaa
     6061 ctaatgctct tgcaatggat attctctgtt tttgaccacc tgacaaatta tttccacctt
     6121 gtgctacaaa tgagtcatac ttatcttcca tatcagaaat aaattcatca gcttgagcta
     6181 tacttgcagc atgctttact tcttcaatac ttgcatcttc ttttccatac cttatatttt
     6241 cttctattgt cccactaaat aaaaaagctt tttgtggtac aaacccaatc ttatctctaa
     6301 gttctttttg aggaatcttc ttaatatcct gaccatttat agatatttct ccagattgaa
     6361 tttcaaaaaa acgtggtatt atatttgcaa tagtagattt gcctgagcca gtgcttccta
     6421 taattgcagt tacttctcca gactttgctt caaatgttat attatttaaa acaggttctt
     6481 ctgattgtgc atatgaaaat gttacatttt taaatactaa atgccccctc tctgagacaa
     6541 catcttcacg accatttcca tctacaattt caggttctat atctaatatc tgatttactc
     6601 tatctgcaca tgcacctgct ctaggtatgt acataaatac cattactccc atgattaaat
     6661 agaataaaat taagatacaa tattcaacaa gtgccattat atgacctatt tccatatttc
     6721 cattacttac tctcatcccg ccaaaccaaa taatacttac tatacctaga ttcattatca
     6781 acattacaat aggcataagt actgcaaata ttttatttat tgaaattgct gtttcagaat
     6841 aatctattgc agttctatca aatctctttt tctcaaaatg acttctatta aaagctctta
     6901 taactcttac tccagttact acctcacgta acaccctatt catattgtcc atttttactt
     6961 gcatcatttt aaacaacttg ataagctttt taccaactaa aaatgctgat aacatgaata
     7021 gaaacattac tacaacaata ataactgtca ttactttatc tatagaatat gctaaaaata
     7081 gaccagaaac agttataatt ggtgctggta agactaattg aataaacata aacactgact
     7141 gacctattaa agtaatgtca ctagtactcc tagtaatcat tgatgctgta cctatattat
     7201 taaaatcatt tattgataaa tcttgtgatt ttacaaatac cgcttctcta atatctctac
     7261 aaaatccaga tgaaagattt gctgatactt tacaaactaa tacagctgat aaagctgtta
     7321 taccagctac tatcatcatc attaaacctg tttttacaat ataatctata tctcccttta
     7381 ctacaccatt gtttactata tttgctgtaa gtgttggtat atacaaagtt ccaagtgttt
     7441 gtaaaaacgt aaatatcaaa acgataacaa ttaaaactct atatggtttt aaaaaatgaa
     7501 ttaatttttt catacaatca cactccttta caaaatatta tgataaacta tactgtaaca
     7561 ggatagtcaa ggaggaatta taattatgag tcaagtttca aaaaattatt ttacaactgg
     7621 agaatttgca aagatttgtg gaatcaataa aaaaacactt ttccattatg acgatatagg
     7681 tcttttctca ccagaattaa agaaagaaaa tggatataga tactactcat accatcaact
     7741 tagcattttt ggtataattt cttcacttag ggaagtaaaa atgcctttaa aagaaattaa
     7801 aacttatata gacaaaagaa cacctaattt actaattgag cttttagaaa agaaaactat
     7861 tgatattaaa aatgaaattg aaaaactaaa taatattcaa gctctaatgg aatctactat
     7921 ttcatttact aaaaatgcct gtaatataga tgcgaatact attattttaa aagaacatga
     7981 agaagagtac ttagtcaaaa cgcctattat atataaagaa cagtttttag gagatgagga
     8041 agaaaatttt ttatatgaat gtattaactt tatggacaac tatgagttga gtgattatgg
     8101 tacgattggc tcaatcatta aaggtgaaga tataattaat aaagattttg aatcatattc
     8161 atatttattt acaaaagtta ataaagagta caagaaatat cctgtatcaa tcaaagcaaa
     8221 gggactttat gtaactgcat atcataaagg aagttatgaa actatttaca aaacttatga
     8281 aaagttatta aattttttta atcagaataa tttatgtatg ggggattttg tttatgaaga
     8341 atatctttta tatgatataa gtgtgcgaga ttctaatgaa tatctaactc aaatctcagc
     8401 agaagttaaa atgtaaaagg agacaaatta ttatgataca aagcgagata tctgatagaa
     8461 gtaattataa aaaaataaat actctagaag ttgtaatatt ttttgtagta acttatggta
     8521 tatcactgct gtttgggata cctctatatt ttaaagaata tatgaatcca caaataatgg
     8581 taacatttat gactatatta ccagcatcag gagtttcaat agcaaagatt tataaacata
     8641 aaaagagtga tgaacatatt atacttcact gtgtcataat agctgacttt ttattatctc
     8701 tatttctagt tactttaaaa gcatttggag caatagatga tgaaactgta atcttaggaa
     8761 tgggtatttt agtcttattt actggaatta taacgatatg ttatgcatgt ttagaaggta
     8821 aagagcttta cccatttaag aatgtaaata aatctattat ttgtattttt atttttataa
     8881 taataattaa tctaagtaaa tgtataatgt taggatggaa agatattaat tttataaatt
     8941 tgattatgaa tatattttta atttttccat atatagcaaa tcaaagtatc cttttctttg
     9001 gagaagaata cgggtggaga ggttttcttc aagaaaaaat gcaaacaaaa tttggcaaac
     9061 gaatgggtgt tatattatta gggattatat gggaattatg gcacatacca ctatggttta
     9121 caatttatca tgtagatggt ttaggtatat tacttagatt attttctgtg attagtcttt
     9181 ctatattctt tggttatgtc tatatgaaaa ctaaaaatgt atggtcatgt gtagtaattc
     9241 atttaattaa taatactgtt cttttggata ccaatttaaa taatgctttt ttaaacttta
     9301 atacttcaag ccttaattca agtgttatat taggatgttt aattttttct gtactccttt
     9361 cattatttat cttctctaaa gagtacaaaa aggaacagct aatatattaa aataaataaa
     9421 aatctttata aaaccaactg tttaaattta taaaatttat ttttataaag gtgtaagtaa
     9481 acttaaatta atataaattt acttacacct ttgcttatgt taattaacct cactgaaaca
     9541 tataaaaata tacaaagtaa catattttat aaattaaaca gccacaagta tatataaatc
     9601 ttactctcac ataaaatcta aattcaaatt cactgtacat atagttcttc ttcaaataaa
     9661 caatatgaat ttttacctga tgcttttata ctataaagtg ctctatctgc atttttaaac
     9721 aattcaaaaa atgaagttcc atcttgagga taccttgcaa tacctatact gacagaagca
     9781 tcaaaactaa aactcaaatc agtgtaatta tctgaaagta ctctatttaa ttctttagct
     9841 tttttatgca aatttacttc tgtaacgtaa tcttgcatat atacaacaaa ttcatctcca
     9901 ccaattcttc cgactaagtc tttactacca aaactagcct gtatcttatc agcaaatgtc
     9961 ttcagtacaa aatcaccaaa tggatgacca aaattatcat ttatttcttt aaaatcatct
    10021 aaatcaataa tgaataatgc atctttttta tcacctgtat tcataactat agtttcttcc
    10081 actaattttt caaatgtgcc tttattgtat atattggtca atgagtcact ttctgctcta
    10141 gttcttaact cttcaaattt ttttatatca ctatctacat ctacaattac tccgacaact
    10201 cttaaaagtt cattttcatc attataaatg cttgtaattg atgttctaca ccaaatatat
    10261 tcaccattag atttttttaa acgatatact gcttgactgt gtatattctt ttttttaata
    10321 ctttcaagaa aactttcaaa tatagcttta tcttcagaat gaactaagtc tttatcttta
    10381 atttctttaa aaatattctt acatgcattg ttatatccaa atttttcagt ccaaacctgc
    10441 gatacaaacg atttattctc tttgatattc cattcaaaaa cgacagaatc ggtctgttca
    10501 acaataattt tataacactc attactaagt cttaattctt ccttatgaat tatttctttt
    10561 tcaagtattt catatttcat agatagttta gttttatcta taataaattt aaataaataa
    10621 aagacaataa aattaatata taaaatacca acactagata ttaagcttag aaattttaaa
    10681 ctttcaagac taaaaaattt acttacatca aagatagaaa cagacagaaa taaactaaat
    10741 ataggtatta aaaataaagg gagccaatac tttatgggca tatctaaatg tacatctttt
    10801 ttaaatagac aaataatttt acaagtcaac aaaaagacaa ttttagataa aattgtcgcc
    10861 aatactctta tactattatt ttcaaacatt atttcaaaac cttcttttac aaataaaaca
    10921 aaaatacttg taactacaat ttctgacagt ataaagaata tacttaataa aacagtagta
    10981 aacaatcgtg tctttaaatt tcctttatat aaaaatgatg ttgaaaaaat aaggatgaaa
    11041 attaaaggca ttgtgatttt agggtttaaa gttattaaat tacagaatcc taaacatatt
    11101 ataaaaccca gtttagcata tcttacaatt tttatatacc cttctttctg gcctaaaaag
    11161 tctgataaaa acatatggat gacaaaagct tcaaatacaa ccgttactaa atttgtaata
    11221 ttaaaaatca gctgttcttt catacatatt ttttataaaa taagaagctt acttaaacct
    11281 aaatacactt cagattttac ttttcctttc gtatctatat ttaagcattt actttaaatg
    11341 tcaactatcg taatatatgt cagcttaata ttgttctttt ttcaacagta tacaacagat
    11401 atatataaaa aacaacttca aacatctcta tattttctct aattttttta caatttaaat
    11461 aatatagtaa atttagctcc ttttttgcca ttttctacgt atatacgtcc accatgctta
    11521 tttacaatag atttaactat tgataatcca attccatgat ttccccctac tcccttatag
    11581 aatctttcaa aaacatgagg taaatcttct ttcttgattc caattccatc atcttctatt
    11641 atcacaacta tatttttttg attatactta cacactattt ttatttcact cttagcgtat
    11701 ctcaaagcat tttctataag attcataaat gctttagaaa tgtttttttc atcacattca
    11761 aataaaacca tttcttcatc aaattcataa ataaatttta tccccttatt tatagctctt
    11821 acattttgcc tagctccaca atttgaaagt acttctctta aatcacattc tacaagttga
    11881 aaatctttag ttatattatc tattttagaa atgtataata aatcttcaac catatcactt
    11941 aacctatcac tttcttcaag aattatatca cttgcatgtt tactatctat aacattatat
    12001 tttatagctt ctgcataacc tttaatagac atgagtggtg tccttaattc atgagatgca
    12061 ttttgaaaaa atattttttg ttcattatca tacttatcca gatactcagc actcttattc
    12121 ataacagttg agagctctac taattcttta tctgaaaagt caaagtagtt tcttttgaaa
    12181 tctccttgac caattttttt tgcaaactca cacaactctt ttatcggttt agcaattttc
    12241 tcagataaaa tcacagctgt aaatattgct aatattccag caagacacat tacagatatt
    12301 aatacaatat ttatcttttg tgctagattt agagtttttg atatgtctat aaaaagtata
    12361 agatacttgt cttgttcacc atatatacta gtaattttta cagttgaaat ataataatcc
    12421 ttatttctgg tagatatccg tgtattttca tctgaattta aatctaattt ttcattttga
    12481 atacactccc taatcagctc catctcatca acatttttta agaaatcatc ctctcttgtt
    12541 ggaaatatca ggttatactt agaatcaact accatagcat cagcatctga ttgtgtttct
    12601 gccatcctaa ctttatcttg tacatttctc ataaaattag ataatacttt gggaggaagc
    12661 tttccatcat attgtggtgg cttaataggt ctaaaattac tatccatatg ttctactgtt
    12721 tctgtagact ttgtcaattc ttcattcgca gttgttttta tatatgtatc aagaaataca
    12781 ttaaaagata ctaatattat cataaatact ataattatca aacaaagtat gctcaacaaa
    12841 atcctagttt ttatgcttat atttttcttt gagaacttta atttaaattt ggatttaatc
    12901 tttccaattc tattcttgtc atttccattc ttcatcttaa cttttctcct tcaacctaaa
    12961 tccaaacccc catacagttt ctattaaaat atttgtatct aaaatttttt tacgtaatct
    13021 cttcactgta tcatcagcag cccttgtctc aacttctata tcatatcccc atactttgtt
    13081 taataattca tctctggaaa ctgctctatc tatattttca aacaaataag tcaataggtt
    13141 atattcattt ggtgtaaggt ctatatttac atttttactt gtaacgattt tattcttttt
    13201 atttattatt acatccccaa attttaattc catatctatc gagttactat atttatcata
    13261 attttgtttc ttttcaaact ctatccttct aaatatagat tttactctca taactaaaga
    13321 cattgggcta aatggttttg taaaataatc atcactacct aaggttatcc ctgtaatata
    13381 atctatatct gtatctcttg cagtaagcat gattattggg actgtacttt tctcacgaag
    13441 ttttgtacat acttcaaaac cacttgaacc aggcatcatt atatctagta ttactaagtc
    13501 acactccttt atattgaatt gttctaaaag ttcatcaccc gttttaaaat ccataacatc
    13561 atgaccttca tttttcaaga atgtctttac caaatttctt atattatctt catcatccgc
    13621 tatataaatt aacttactca taagctcact ccttaataga ctttttattt aaattaaata
    13681 gtataaatat attgattaaa taattaaatc ccaatagata agataaatct tattttttac
    13741 tatatttaaa ttataccagc tttttatagt acattagttt taaaattgtt tggattttaa
    13801 ggcaatttta aggcaaaaaa taaattatta tagaatttat taaactcatt ttagaaatat
    13861 cttgaactac ttcactttaa ttacctttta attcctttag gtgatatttg aaggtgtagt
    13921 gctttgcata tttttgataa aataataatt caatattcaa ctgtatttat tcattttata
    13981 tcaaaattga tatttttcaa actttttatt gaatattcaa acaattaata ttatagttat
    14041 attaagtact acagttcacc ttaattctaa aaatatttat tcatatataa tacttatttc
    14101 attcttcact aatattatgg atttcttaaa ctaagtgata aaatcagatt ccaacaaaat
    14161 aaaactgaat aaagtatttc tgaagggaga atttaaaatg aaaaaagctc ttagatatct
    14221 tttattttta atcattctat tgcttatgta ttttttagct gttaaattaa attatgattt
    14281 ctatactcaa tttcacactg gttatatgca agactttaaa agatacattt ttattaattt
    14341 aatttcctct ggtggtattg gtttattact aggaactgaa ttattgatta gagaatacaa
    14401 aaatgatggt agttggtata ttgatattcc aagattactt cttcttagtt ttccatcttt
    14461 tttactatca ctgatgcctg tattcttctt tatgtttcca gttggtaata tcccaattat
    14521 aggtaatttt attatgcttg atagaatccc actaaacata ataattttta acatcttatt
    14581 tggatacttc ttgataacga gttttcgtaa aaagtaataa acaagtaatt taaacaataa
    14641 taactaattt aaataaaaag aagtaagtaa tttaaataat aatagctatc attaaataaa
    14701 tttgattatt caatgatagc tatttttcac ttaaactaca ttctattctt atactaattt
    14761 ataatctttt ggattaagtt tacttgctgc tgcttcatct attataacta tagtgtcttt
    14821 atgaagttga agtatagaac caggaacttc tggagttata tctccataaa cagtattgta
    14881 tactgcatca gctttttctt ctcctgaagc taaaagcaat attttcttag acttcaatat
    14941 tgaacctatt cccatactta cagctttttt aggaacatct tctcttgatt taaagaatct
    15001 tgcattagct tctattgtag attctgtaag ttcaactaca tgagttcttt ttgcaaattt
    15061 attatcaggt tcattaaaac ctatatgagc attacgacct attccaagta cttgtacatc
    15121 tataccacca gctgcatcta tagaagcttc atagttcatg cactcttttt caatatcatc
    15181 agccattcca tttggtagat gagtattttc tggctttatg tttacatgat taaatagatt
    15241 ctctttcata aaataatcat aactttggtc attagatatt ggtaacttat aatattcatc
    15301 taaattaaag ctcatcacat ctgaaaaatc tactatatta tcattgtatt tttttactaa
    15361 atctctatac atacctactg gtgtacttcc tgtagctagt cccagtattg aatttggttt
    15421 taaaactatt tgagctgcta tcatctcagc tgctttttta ctcattccat cataatcttt
    15481 gcacactaat actctcattt tttcctccta aattttaact actactttaa ctaatgtaag
    15541 tttaatttat tattttttat attgctttta ctgtctttaa agtttgtttt gtatactata
    15601 tatttttata tactacttcc cctaagcaaa ttgtcatctt acaatctaaa ttctcatcaa
    15661 acacagctat atctgcatcc ttacctattt ctaagcttcc ttttttgtca tttattccca
    15721 aagatgttgc tggatttaaa gatgctaaat taattgcttc attcacttta aggtttgtat
    15781 tatctaagaa gttaaatacc attttattta aagacaacac acttccagct aatgctccat
    15841 tttcaagtct agcttcattc cccttaacta tgactttctg acctccaaga gaataattgc
    15901 catcttcaag accaccagct tctatagaat ctgttattaa aatcagtctt tctttccctt
    15961 tattgtttag tataaactga aataaatctt tatttatatg gattgtatct gctattagct
    16021 cactgtacac attagttgta agtgctgcgc caacaacacc tgggtctcta tgatttaaac
    16081 cagtcattgc attaaacatg tgagttacat ttgttactcc taaatttata gcttctttag
    16141 cttgctcata atttgcatta ctatgaccaa tagataaaac tatatctgta catcttttta
    16201 tttctctagt aaaatcaaat tcaatatctt tttctggagc ataagttata acttttataa
    16261 catctttgta ttcttttata aattcatatt ttgaatttat tataaacttt tcattttgtg
    16321 ctcccttata attttcattt ataaaaggtc cttctaaatg tgctcctaat acttgtgccc
    16381 cttcaagctt tctattttgg gcttttttaa taacttcaag tgcatcataa acatgttctt
    16441 tatccatagt cattgtagtt ggaagaaaag atgttactcc atgtctcata attgattttg
    16501 aaattgtttc aatagattca tcagttgaat ccattgtatc ttttcccatg tttccatgta
    16561 tatgaatatc aataagccct ggagatatat attttccatc agcatctata acttcacaat
    16621 cttttggtac actatcagct atatcaatta ttttttcatc aaatactaat actttatttt
    16681 ccaagatttg attttttaat attatctttc cattaattaa acatttcata ttattcacct
    16741 actcactcaa tactatttca tattgatatt tatctgttct aaatattgat atggtatatt
    16801 ctatagggct accttctcta ttataggaat ttctatgaag tttaagagct aaagcacctt
    16861 catctgtttc aagaagctta ctttcttcat ctgaaagtat tactggctcc attacctgtt
    16921 cagctttttg tatcttatag ccatattttt catttaaaac cctatataat gaagaattat
    16981 ccaatatccc ttttgttaga cctttacaca tttcttctga tagatatact atttctaatc
    17041 caaatggctc tccatcagta tatctaagtc tgacaatctt atatatattt gtagtattat
    17101 cacttattcc aagttttctt ttaactaaat catctggtag ttccatctca aaggataata
    17161 tatcagtttt tatgtttact cctttttcct tcatgacatc agtaaaacct tttacatttg
    17221 aaaactgata cttctttttt tgctttgcaa cgaatgtccc tttaccttgt actctataaa
    17281 gaagtccttc tgtgactaat ccaacaatgg ttttgtttac tgtcatccga cttacatttt
    17341 gtatgttaca aagttctctt tctggcatta ttgcatcacc ctctttaagc tcaccagttt
    17401 ctatcatctc tcttattatt gaacttaatt gagtatgcaa tggtatgtta ctagatttat
    17461 ttactatttt catatagtca ccacttttcg atatggtata taccaatttt ggtatatacc
    17521 aattatactt taaaagaaat tttatttcaa tactttttaa cttattttgt aaaaatttta
    17581 ataaaaccta aagacaaaaa agaaattgga gtttatacta tataagaagc tagagaattg
    17641 aaaaatacat aaataactat attatttatt gtaattttca ctaatactaa tatagatact
    17701 gtgacaaact actacataag tatattatgg ctaaattata aatataaaat gagaggatga
    17761 ttttaatttg ataaacatag gtgattttaa taaacttaca gtaaaaagaa aaactgaatt
    17821 tggatatttt ttggatggac aaactaataa cacaaaagac gatatacttc ttcataatag
    17881 attaatcgga aaaaatgaaa taaacatagg tgatgaagtg aatgctttca tatttaaaga
    17941 ttctgatgac agaactgctg caactttaat accgccttta gctaaggttg gggatgtagc
    18001 acgtttaaaa gtagttgata atacagatat tggtactttt atagatatgg gacttcctaa
    18061 agacatacta gttcctttta aagctaagac ttatccacta tttagagacg aaaaatattt
    18121 attttatata tatttagaca aaagcaaaag aattgctgct actacagata tagattcata
    18181 cttattaaca gaccatactt acaatgtagg agatattgta actggagttg tatatggatt
    18241 ccaaactaat aattctgcaa tgatatgtgt agacaacaaa tatgctggtg taatacttca
    18301 taatgagtac tttactgaac taaaaactgg tgatgttctt gaaaatttac atgttataaa
    18361 aatatatgaa gatggaaaac ttggtctttc tcctagagga aatagaaagg atgaacttga
    18421 tactttagaa aataaaattt taagttattt agaaggttct gatggatata tgagatttaa
    18481 tgataaatct gaccctaaag atatttctat attatttaat tcaagtaaga aaaattttaa
    18541 acgagctctt ggagtgctta tgaaaaaagg attaatttat caagatgaag aaggaactta
    18601 tctaaaatag tatatttaaa atattttaac ttaaatgctc acgttgtcat ataatgttta
    18661 gattcacatc atctttatag aaattttgta acataacaaa aattttataa tataaaaaac
    18721 gaagtaaaaa tgaaagatat tttctatttc atatttactt cgatttttta tttaaattac
    18781 tacctctatt taaattacta cttatattta ttgtgcattt actacctcta ttatgtctaa
    18841 ttcacaaatc tttttaattg gttttcttac tgcaagcaaa gttattatta tcatagttac
    18901 tacaatgata aacagtaaat caaaaggtat ttgccagtct atatggaatt ttaatgttac
    18961 taaggataca aatatatatc tatgtaatat taatccaaat acactcccca ataaacagcc
    19021 acttacagca tatgtacttg attctactat taccattttt cttaattgct tattggacat
    19081 gcctattgca cgcataatac catagtaatt tattctactt gtaacgctta tattcatact
    19141 gtttatgata ttaaacatac ttataatcgc aattataaat acaaacccat atacaaaaac
    19201 tgccatagtg taaaatgaat ttttagcctc taagttacct tgtcgcttat ctcgaacctc
    19261 taaattatca tctagaacat ttcttatatt ttggattaaa tcttcaggag catcttcatc
    19321 aacttgagta tcaattatta tatagtcttt tatatctgtt atactagtaa aagtattttc
    19381 agatgttata atatttccaa tatatttgtc tttaacatca aatgaaattg aagaaagtat
    19441 acctgctacc tctatctctt tcttagaatt tccattttta atatatatct tatctccaat
    19501 attaaaccca tgaccttctt ctaccaatcc gatattttct gactctaatt tatcttcatt
    19561 gatttcccct tttactagat actttttggc ccagtcaaat tgattttttt cataagaaat
    19621 tagctgagat gttgcctcct tattattgta tgtagcctct acatccgtca tcattctact
    19681 atatactttt tttattcctt tgatattact tatttcatta atatcgtctt tagtaagaac
    19741 agacttaaag tcagcgtcaa caatagatat atgagtagct tctggtttta taggtttaag
    19801 tgcctgatac ataaagtcta taaatacact aaaacctaaa aacaatacaa tactaatagc
    19861 aaatgaagaa gtcataagta taaaagattt ttttcttgaa aaggcatgat ttattcccat
    19921 agaaacatca attttagaac ttcctgcgtt agcaatttta cttgcttttc cactagttaa
    19981 aatttctttg aacattagat taccacgtat agcacttagt ggagacaccc cagaagcttt
    20041 ttttgctgga acaatagctg ataatattac tgttaaaaat cctactatta ttccaaacac
    20101 aataccaata aagctaacct ttaaaattgg catcgaacca aataactctg tattcacata
    20161 tttgagaaat atagaggata taatggttac aatacatcct aataacaatc ctagtggaat
    20221 agcctttaca cttagaatta aactttctaa aacaacaaat tttttgactt ggagttttga
    20281 tgctcccaag caacgtaata gtccaaaaaa ctttattctc tccattacac ccatattaaa
    20341 gctattatat atcatgataa tacttgctat taacacaagt ataaaaagtc ctcctgctgt
    20401 taagtaaagt ggtttagaat aatcagagtc accttgacct ataatagaaa gtaatttttc
    20461 atttttaccg atttgtttat cacttaaatt gaaggtattc ttaatatcag aagtagcttt
    20521 tctaatatta accttgtctt taaatgtaat ataataataa tcatatgttt ctgatttttt
    20581 gatagcttgt gtacctaata ttgaaagcat taatccatgc ttatctgagg ctcttaagct
    20641 tgataatgat gaatattcac ctactatctt ataattttta tttctaccat ctgctaattt
    20701 tattttgata aaatctccta cttttagatt aaagctttta actgcatcta catctattaa
    20761 agtctcattc tccacaacag gataacgacc ttttattatt tctagaccta attgttttgc
    20821 taaatcttca tctaaacctt gtatcaaaat ctcctttgat ttaaactctc cattatcaat
    20881 gacttgtcct ctaccatagt gagctatatc tactctacta cttatttctg atatagcttc
    20941 ttcattaaga tttttaagta ctatgtgcca atttccatca tcttttataa attgtacttt
    21001 ttgacattgt atagccatgt ctgctagtcc aaaaatagca gtaactaaac atactgcaat
    21061 gacaatacat aaaattgtag ttctattttt tttcttatgt acctttgcat attgtggtac
    21121 tagttctaaa tagcttctca ttctctattc acccctaggt ctttaagcat tccatcagaa
    21181 acctgtagta tcctatctgc tgtagaagct atatttgtat tatgagttat cataataata
    21241 gtttggttat atttttctgc tgtcactttt aaaagtgaaa ttacatcact gctattttta
    21301 ctgtcaagat ttcctgttgg ctcatctgcc aatataattg atggctttgt aatcaatgct
    21361 cttccaattg caaccctttg ttgctgacca ccagataatt gatttggaag atgacttttt
    21421 ctatctttaa gacctaaaat tgtcaatact tcatctagat aaatagtgtc tggttttttg
    21481 taatctagta gtaaaggaaa tattatattt tgttccacag ttaattcggg tataagatta
    21541 tatgattgaa agataaaacc tatattacga cgtcgaaaaa tagtctgttt ttcttctttc
    21601 attgaacata tttctttacc atcaacaaat atttttcctg aggttggagt atcaagacct
    21661 cctaagagat ttaatagtgt actctttcct gaaccagatt ctccaactac agtcacaaac
    21721 tctccttttg aaactgtaaa ggatacatct ttcaatgcct ctacctttgt atatccattt
    21781 ccatatgttt tacataaatt attaaccaca agaatatcca ttttctctct cacctttcta
    21841 attgattaat ataataataa tctatatacc ttactgcaag atgtatttta tcttacatat
    21901 tagtaagctt taaaaaactt atggtgaatg tacttccttg tgatagttta ctttctacag
    21961 aaatagtacc attatgttgt tccacaatag atttaactag tggtaatccc agtcctaaac
    22021 cttgattatc ttttgaaaac ttacttctat aaaacctttt aaatatatga tgaatatcct
    22081 caacatggat tccatttcca gtgtctttaa ctgaaacaat ggtggttata gtcgtctcaa
    22141 accactctat cgttatttta tcattctctt ttgtattatc taatgcattc tttataagat
    22201 tacttaaagc ctctgacatc cattcataat cgcagaacaa caatgtattt tctgaaccat
    22261 ttatagaaat aacctttcct tctttttctg ccctagtttc aaaactatac aaaattttga
    22321 caattaaatt tttaagatta acattatttt tattgagaac tattgttttt gaatctatct
    22381 ttgtaatctt tagtaaattc ttaatcaatg tttcaatgcg ttctagttca ctttgacttt
    22441 tttgtacaaa agcttcaact acatcactat tatgactttc ttcttgtagt atctgattgt
    22501 acattattaa agctgtaagt ggagttttta attgatgcga tatatctgaa attgtatttt
    22561 taagaaattc tctattctgt ttttctttta atatatgtgt atttaaagat gtcgacataa
    22621 catctattga actaaataat tttgataagc taccttcttc atatgtattt agtctttcat
    22681 ttatatttcc attcataaat gaatcaattt tataactagc ctcttctatc ttactttgtt
    22741 gcctaaaaaa atacatatat agtatgaaaa taatcatagt aaaaaatact aataaataga
    22801 tagacatact ttttatatat ttactttgaa acaattttac acttggtgta aagctaacat
    22861 ctatatcaaa tccatatcca gctgtatcta gtatttcttt tccttttaaa atatatttat
    22921 cttcatgtgc aacaaatgct gatggtattt tagatttatc aacacctata ctatataagt
    22981 atccagctat attataatca tgttctatca acgttttttt ataattgtta gttatactta
    23041 taagcactat ttgccctact ataataaaca ttactaaaat agatataaga attaaaaaaa
    23101 acatttttat atctttatta gaaaatatct tcatattaat accctcactt aattacattc
    23161 catttataac ccattcttct aacggtcaat agaaacttag ggttttcagg attatcttca
    23221 attttatttc tcaatcgtct tacatatact gataatgtat tgtcatctat aaagctacca
    23281 tttccatccc ataacttgtc caatatgttt tttctagtaa gtacaatatt tttatttttc
    23341 ataaataagc acaataattt atattctgct gtggtaagtt ccacctcaaa ttcaccttta
    23401 aaaacacgat tttcaagtaa tttaatagta atattatttg agcttaattc attcacatta
    23461 cttgcaacat tataacgtct aagtaatgct tttattcttg aaattaattc atttaattta
    23521 aaaggttttg ttatataatc gtcgccaccc atatcaagcc ccatcactac atttacctct
    23581 tcatctgaag cagtcaaaaa tataatgggt atattagaaa cctctcgtac tttttcgcaa
    23641 atctgaaatc cattaccatc aggtagggat acatctaaaa gcaataagtc ataatttttt
    23701 tcctcataca tttgcatagc ttcttttact gtccttgcta tgtctatatt aaatccactt
    23761 ttttgtatag agaactctaa tccatatatc aagcttaaat catcttcaac taacaatata
    23821 ctactcattt aatcacctct attttaatta tttttacttc taatacatta ttacacttaa
    23881 tttattataa ttataatttt actttttcaa aaactcaata tattaaaaca aatgttttta
    23941 caaaagacta cacaaatcaa cagtctaatg attggtatta ctcctataac tctcatcaat
    24001 atatacacta tctcaagtag ctagaaatca gcttgacctg tattatttta tataaaaaat
    24061 agaaaatttt ataattcttt atctgttaat agttaaacag agaattgaaa ataatgattt
    24121 tgtagttgga tttgacttga ggtataaaaa gacatggata tataagtaga atataaataa
    24181 catattttag attttcacaa tttgaaatag aaagagggat gagaacatat ttaagcatac
    24241 aagaataact gaactaccaa gtaacaaaac aggtggtatg ctataaagca caccacccct
    24301 tattctcaac accatatttc atgttttgca tataactaat tttgcataaa attagttcca
    24361 gcgttttaat gttggaaaga aattgttcat cattgttctt gcttcatctt cagtcatatc
    24421 cttattactt tgaagcatat gagggataca aacttctatc atctcttcca tacttatatc
    24481 agctgtaaac tcaccatctt taaaacaata actacaataa tcagtacttt tacttccatc
    24541 tttatttgtc ccatataatt catcagtttg tcccattggc attgcacagc attggcaaaa
    24601 tttagtttct tccatcttaa atcactcctt atatttttct ttggctccta accatattca
    24661 aattatatca agactaatat gacattatta tgtcatatta aaaataaaaa gactaataaa
    24721 atcatcccac tgtacataat ttaattagtc ttttatactt ttttagtttt taataaattg
    24781 taaaaaatta tattctattt actttggttt ttatcgctgc ttctttaact gccttagcaa
    24841 cattttttac tacattctta ttaaatacat ctggtaatat atactctggg catatttctt
    24901 catcacttat tgtattagca attgcatatg cagcagctat cttcatttct tcatttatct
    24961 ctttagcctt aacatcaagt gcacctctaa atattccagg aaaagccagt acattattga
    25021 cttgattagg gaagtctgaa cgacctgttc ccattataaa tgctccacct aattttgctt
    25081 cttctggcat tatctctgga gtaggatttg ccattgccaa tattattggc ttctcagaca
    25141 tactttcaac catttccttt gtaacagctc ctggacctga aacgccgata aatacatctt
    25201 ttccttttat aatatctttt aaaggtcctt tttcatttct tatatttgtt acttttaaca
    25261 tttctttttg aacatagttt agattttcta tactagcttc caatgctcca cttctatcac
    25321 ataagattat atttttaaca ttcatattaa gtagtatctt tgctatggct ataccagcag
    25381 ctcctgctcc gtttataact atttctaaat cttctatttt tttattttca attaacttta
    25441 ttgagtttat tatggctgct gcaactatta ttgctgttcc atgttgatca tcatgaaaaa
    25501 caggtatact taaagatttt tttaatctct cttctatctc aaaacatctt ggagaagata
    25561 tgtcttctaa atttatacct ccaaatacag gttccatcaa ctctacagat ttaactatct
    25621 catctacatc attagtcttt aaacatattg gaaaagcgtc tacatctgca aattgtttaa
    25681 ataatatagc tttaccttcc ataacgggta tagatgcttc agcaccaatg ttacctaatc
    25741 caagtacagc acttccatca ctaactactg ctactagatt ccctttagaa gtgtatctat
    25801 aaacatcatc ttgattttca tgtattttta aacaaggttc tgcaacacct ggtgtatatg
    25861 ctgttgataa atcatcctta gttttaactt ctaccttgct ttctacactt actttccctt
    25921 tgttgatttc atgcatttct aatgccaatt ttgcataatc cttagacatc aattttcctc
    25981 cttaaaatat acacaactat ttttaatttt gatattcata tttatttcta aacattattt
    26041 acattcataa attgctcgac cttgctcata tatattattt ccatagctat ctattgctac
    26101 tgtaagttta aggtcttcta ctacaatctt tcttatagct tcggctccta aatcttcata
    26161 tgcaataatt tcacatgatt ttattgaatt tgaaatatac gctccagctc ctcctattgc
    26221 agcaagataa actgctccgt atttttgcat accctctata acttcatcac ttctttttcc
    26281 cttgcctatc attaatctaa gacctctttc taaaagaggt atagtcaaat catccattct
    26341 ataactcgtc gttggacctg cagcacctat aacttctcct ggctttgtag gagtaggtcc
    26401 tacataatat attccttgac catatacgtc aaatggtaat tcttcacctt tatttataca
    26461 atctatcaat cttttatgag cagcatctct tgctgtatag agtataccac tcaaactaat
    26521 tgtatctcca caatttaatt ttgctatatc aatttcattt acaggtgtag ttatttttat
    26581 cattttattt tcctcctaaa ttactacctt cttatgtctt gatgcatggc agtttatatt
    26641 tacaacaaca ggtagacctg ctatatgtgt aggaaaagtc tctatgttta atcccaaagc
    26701 tgttgtagta ccacctaagc cttgaggtcc tataccaagt ttattaatag ctgttaatag
    26761 ttcactttca agttttgcta tattttcgtc cttattaaat tcacctagtt ctctaaaaag
    26821 tgccttttta gctatttgag cacatttgtc cacagtccca cctataccaa ctccaattac
    26881 cataggagga catgggttag gtccagcttc tgaaactgta tctattatga attttttaat
    26941 tccttccaat ccatctgatg gctttaacat tttcatctta ctcatatttt cgcttccaaa
    27001 tcccttagct gcaaattcta ttttaatttt atcacctttt accatatcat aatgaataac
    27061 agcaggtgta ttatcttttg tatttactct attaattgga cttacaacag attttcttaa
    27121 gtagccttct tcataccctt gtcttactcc ctcatttata gcatcagtaa gtgtgtctcc
    27181 ttcaattaaa acttcttgtc ctatttcaac aaaaaataca gccataccag tatcttggca
    27241 tattggtata tttttttctt tagctatttc agcattttca actaatatat tcaatatatt
    27301 ttttccaact tcactctttt cgcttttagc tttctctttt atacaactta aaacatcttc
    27361 tcctaaatat aggctcgctt caatacaaat tttttttact tgctcgacga tttgttcaga
    27421 ttttattttt ctcaagcgaa catccctcca ttattttata aaaaatattc gtgattatac
    27481 ttacacttta ccattgtttt taaaatttct caatattttt ataccaatta cacataagta
    27541 actgaatatt tattttaaaa ttattattca ttttagtaat actacaacac ttattattaa
    27601 gaatataagc tctatatttt atatgtaatt cttatttttt tattacatct acaaattcta
    27661 cttttataag tctttgtaaa tcaaatggag atagttctat ttgatatcct atttttcctg
    27721 cactcacaat aattgtatct aaattctttg cactttcatt tacaaaagtt ttataaagct
    27781 ttttcatgcc tataggagaa catccacctc taatataacc tgtaagctta tttatatctt
    27841 ttacatgaat catctctaca cttttctctt tagctacttt tgcagccttt tttaagtcta
    27901 ggttttcatg tactggtatt acatatacat atatattctt actaactcct tgagttacta
    27961 atgtcttata cacttcattt acatctcttc ctatatcatg tgccacctca acgccatcca
    28021 catgttcact tttcacttca taagacatta ctttataatc aatcttattt gaatctaata
    28081 ttctcatagc attagtctta accatacatt cacttccttt ttataaaata cgaacattat
    28141 acacagtttt gaatattctg tactatttta atatagtctt atagatttta accaaatata
    28201 ttttaaaacg acagtattat tataactcta tatcaacttc aaaacttcat ttttttctaa
    28261 ttccttagaa ttttttaata ttttttttat tctaattgga gaatctccta aaataaatat
    28321 cctatctgct aaaaacaatg cttcctcaat attatgagtt acaaataaca cagttctttt
    28381 ttcttttctt aatatatttc tgaaattatc cattataagc tgtgtattct ttgcatcaat
    28441 agatttaaaa ggttcatcca ttataatgtt ttttgatgga tatataaaag ctctagcaat
    28501 atttactctt tgtctaagac ctccactaag catctgagga tagaattttt tataatcact
    28561 aattccaacc aattccaagt attcatcaca aattttgttt aattgagttt tattatagta
    28621 tttatttaca actatcttta tattttcttc tactgttaac cagtctataa gtctatcatc
    28681 ttgaaatatg taactagctt caagcttatt tcctacttta tttaaatttt ctttgatatt
    28741 tagattgttg gtgtcatttt taataatccc acttattatg ttcaagagtg tagtttttcc
    28801 acaaccagat tttccaagga tacaatttac ctcattcaca taaaaatcaa tattaaaatc
    28861 tctaaatata acatgactat catatctttt atttatattt tcaatactca accttatctt
    28921 ttccataaaa aagctctcct tgctaaaaac ttttgagcta tttgcaaaat agcagaaatt
    28981 aaaagaacaa taattatcca tgcaaatatt ccagatgtat taaagttaat tttttcagtc
    29041 tgtatcatag ttccaatacc atatgtaggt tgactatata cctctcctgc tatagcaact
    29101 ttcaaactta aagagaatgt tgatactagt atagatataa tttgaaattt tatggctggt
    29161 aaataaattt ttaacacctt atctataaac cttattttat acacattagc catttctaat
    29221 aagttcttgt ctatattcaa tacagctccc aaaacgttat catacaatat aggaaaaact
    29281 atagcaaatc caactataaa aggggtacta tccttttcaa accaaataag tgcaagtact
    29341 accaatatca tagttggtat tgaacgtgtc aaagcattga ttggttttaa taagtttcta
    29401 aaaaatctat ttaaataaga cactattgaa agtataatag caaaaatcat agccagtaaa
    29461 aaactaaaaa aacatctccc catagagtaa agtatgtcta tatagaaact agaatttagc
    29521 ataatctctt ttatactatt aaatacttga cctactgttg gtaaaaaaat atcattatca
    29581 atttttaaag caataatttg ccataaaaat aagaggataa cacaagaaag tgttacctcc
    29641 attttatcta taaattgttc tttatttttt ttatttctca acaaagtata ttgcttcatc
    29701 tggtactttg cctcctaatg tcttatcatc aaaattagct aacttctcat aataattttt
    29761 ataatcttta atcatatcct taactggaat atattttaaa ttggctcttt caagagattt
    29821 tcctattatc tttgattcag tagttatttt tattttttca gaatatgcac ctaattctcc
    29881 aggatttttg tttgcccaat ctatactatt tgataattga cctacaaaag aatcaacaaa
    29941 atctttatta tctcttaaaa attcagactt tacaattaaa gtagattgag gataaccgtc
    30001 ttttgaacca cttacttcct tccatgagtc atttaaactt tttataattt ttatatctgg
    30061 atttttagac atcaaagcac taagtgcagg ttctggaaca atacctgttt taactttacc
    30121 agtagccaat aatggtacca gttcacttgc tgaatttaca taattaaatt ttatatctga
    30181 ataatttata tccttttgta acaaaagact ttttatagtt atgtctggag taagtccttt
    30241 tccagtacat ccaacttcct taccaactaa atcagagtaa tcttttatat tttctgttga
    30301 caccaaataa agtgaaccca tccctacagt tccaacaata ttatatgatg aagttttatt
    30361 atacgctatt gcagccatgt ttgaaggaac tatagctata tctgcatctt ttttcatcac
    30421 tctagtggaa atcgcctcag gtgtcttttc aatgctatac actgtttcat atccatcttt
    30481 aatctctgga ttttcatttg caagtttagc tattgccact gctggtagtc catctggaac
    30541 agctactttc acttgtttga caactgatag ctccttattt ttagagttat cttttcctgg
    30601 tgaacatcct accaaaacaa ccatagtcac caccactaat aatattatag aaatatactt
    30661 tttcatctct tcctcccaaa gttaacgtca ttaaaataat tatatttatt tatactaatt
    30721 tctgttaatt tatcgaataa tccttctttt ttaagtattt ttatttatac tttaatgttt
    30781 tttctttttt ttagttgaac ttttttgttg atttttatta cttctattat ttgtattagt
    30841 ttttttgttt ttatttgttc ctttagatac atttttttta gaattatttc tattaaaata
    30901 aggcttttca ttttttggct ttataaggca tctatgtcca aagccaatta agtcttctct
    30961 tcctgcttca actaatgcag agtataccaa atcataattt ctaggagctc tatattgtaa
    31021 taaagccctt tgcattgcct tgtcacgttt tgatttagga acataaactg gcttcattgt
    31081 aagcgggtca atacctgtat aaaacatggt tgtagataga gtccctggag ttggataaaa
    31141 atcctgaacc tgctctggtt ggtagtgagt atctcttaaa tactctgcta actctatagc
    31201 actatttaag gtagaaccag ggtgactaga cattaaataa ggtatcaaat attgcttttt
    31261 acctaattct tcatttatac caaaaaattt ctgtctaaat ttatcataag tttttcctgc
    31321 tggtttttgc atatacttta aaacttcttc tgatatatgt tctggagcta cctttaactg
    31381 accacttaca tgatgctcta tcaactctct aagaaattta ttattcttat ctgccattac
    31441 ataatcgtat ctaattccag agcgcacaaa cacctttttt attttaggta attttcttac
    31501 agctctaagt aagtgtaggt attcactgtg gtctgcatct aaatttttac atggtgatgg
    31561 agataagcat tgtctatttt tacaagcacc tttagtaatt tgttttttac atgctggtct
    31621 tctaaaatta gctgttggac ctccaacatc atgtatatac cctttaaaat catctaaatt
    31681 tgttattcct attgcttcat caataattga tttttcactt ctactttgca ctgctctacc
    31741 ttgatgaaat gttattgcac aaaatgaaca actaccaaaa cagcctcttg aacttactat
    31801 actaaatttt acttcttcta tagctggtat tcctccaaat ttttcataca caggatggta
    31861 tgtcttctga tatggtaatc catatacctc atctagttct tctctactaa gtggtttttc
    31921 tggcttattt tgaaccagat acttacttcc atgaggttga acaattatat tgcctctaaa
    31981 tgggtcttgc tcatcatatt gaattttaaa agcttctaca tacttcattt tatcttcaca
    32041 aatttcttta tatgatggaa ttaatatata tttatcatat atttcttcca atgtatctga
    32101 aatatagcat gtcccatcaa tatgtctaat atattttggg tcatatccat cattaagtgc
    32161 attggctact tctacaatct gcttttcact cataccataa actaataaat ctgctccact
    32221 atctattaac atagactttc taaccttatc actccaataa tcatagtgtg caaatcttct
    32281 taaactcgct tcaattccac ctattactat tgcaacatca ctgtatgctt ctcttatttt
    32341 attgcaatat actatagtag ctctatctgg tctataaccc attttcccac ctggagaata
    32401 catatccttg tctctatgct ttttacttac agaataatga ttgaccattg aatccatatt
    32461 tcctgcatta actagaaatg caagtctagg ttttcctagc ttcataaaat catctgtagt
    32521 tctccagtct ggttgagcta ttattccaac tttataccca gcattttcta aaactctaga
    32581 tattattgct gttccaaaac tatgatggtc tacataagca tctcctgtaa ctagaacaaa
    32641 atctaattct tcccatcctc tatctatcat atcttgtttg cttattggta aaaacttatt
    32701 ttccatatta tcacctgctc aatatatatt taacaaatat ttttttatac aaaagtatct
    32761 tttaattttg ttcttaacgc ttaattatat cataaaaaat acttaaaacc acaacttaaa
    32821 tgatttatat agacatttta agtctaataa attcaaattg cgattttaag tatatatata
    32881 acacaatatt ttaagtattt tcttcagctt caatactagt tacttcactt ccaaatatta
    32941 aacatgcatc tttcccatca aaccatttca aagtagttgt agttatattt gcctgtacct
    33001 caaacttttt attgcgcata aacgatttat aagtatcatc accatctttt aaatatatca
    33061 tagggcattt tttacaaggt ttatcttgat tattccatat cgctttataa cataaatcac
    33121 ctacatgagt gtttggtgct gactcaagca tttttttatt gataaataat aattcatagg
    33181 tattcctgtc aacaacatat gcccatatat ccatattatc catcacagac ttttgaattg
    33241 ttaaagactc ttctagctta ctctgaatac gcatttttaa taaaaacata ctgagcgttt
    33301 tagatataaa tgtcaaagtg ttcacttcat cattagtcca catacgatta tatgaacatt
    33361 catcaaatcc tatgaaccct ctaaattcat tgccctctaa aattttacat tgtaatattg
    33421 aacgtatgcc ttgtggctta agtacttttt tcacatcact gcctaaattt tcaatatcat
    33481 tacaataaaa taacccatct gcattaaaat ttaaaagata gttgcctaaa tccttgtaac
    33541 aaatattttt aagattatta atttccgagg ttataccttc gttacaccat tcaaatgtat
    33601 ttttacatac tcgattatca tctgaatttt caaatatata catgcgactc acattaaagt
    33661 gtttacctac aatacttaga attaaattta ctgccacttc tatatcactt gattcatata
    33721 gtatatcaaa tatataattt gtgatatgtt ctttaaaaga catctctaca ttatcactat
    33781 ctcctgcact tcttgtatta gttatatatt gtactttatc aattttttta tcatacaatg
    33841 tataacaatt cttacctttc tctttggctg agtaaagagc catatccgca tttctaaata
    33901 gtgtcatata gtccatacca tcttgtggaa acttagcaat tcctacactt aatgatattt
    33961 tataatttcg tcctttacct aaaaaagacc tttcaaatgt ttttaccaat tcttctgctt
    34021 tatttataat atctcttgtt ttagaaatat cttttataaa tacaataaac tcatctccac
    34081 caattctgcc tactatatcg gttgctctaa atagatttgt taggtcattt gcaacttcac
    34141 ttaatacagc atccccatat aaatgcccta aattatcatt tattccttta aagtcatcaa
    34201 tatcaataat cattaaagca ccttttcttt ttttatccaa tgttatgtat tcttctataa
    34261 gtgattgtga tgctttttta ttatacaaat ctgtaagagg gtctttttct gccctttctt
    34321 tcaacttctc agcttctcgt tttgattcat caatatcagt caaacttcct actactttat
    34381 aaggtttacc attattatca aataaagttg ttgcctttat actaaaccaa gcataaatag
    34441 tatctctggt cctaagtcta atttcacact cttcatattc actaccttgt gaagtaagtt
    34501 taattaatga aataaactta tccatatcat catgatatat atctactgat ttaattatat
    34561 tttttgttgc attttcagtt atagggtcat atttaaactt attataccaa ttaggtgata
    34621 tattcatttt atctttttct atatcccatt ctattataat attttgcgtc tgctttagaa
    34681 tgatttcatg acgttcttca ctctttttaa gttcttgctc taataattta gcagaagtaa
    34741 tatctacaat aacacaacaa agtacagatt ttccattatc ataaattatc tttcttcctt
    34801 ttaataaaat ccatataata gtaccatctc ttttttcaac tctttgttca gatgcaaatg
    34861 tatcacttaa tttgagttgt ttgtttatac tattaattag ttctgttcta tcatctttaa
    34921 aaataagttt gattaattta ttatttagct catctttcag ttgtttgcgt gtatatccaa
    34981 tcatatttaa ataaccttca tttaaatata tactatcaaa atcatcattt atctcagtta
    35041 tcactattcc accaacaata ctatcattga tagcctgtat ttcaaatctt tttgaatcta
    35101 actcttcttg tattttcttt agttctgtta tatcagttaa aacaccctga atacactcac
    35161 ttcctttatt atcatttata gacttacttc tctccaaaat ccaaattata cttccatctt
    35221 ttcttacaat tctatattca acttcaattt tatcttgttt tgctaattga tttagaataa
    35281 tacaattaaa cttgtctaaa tcatctttat aaatcatatc tttaaatcta tttttaaata
    35341 ttttatttaa atcttcacaa gagtaacctg taagctctaa aaacccctga cttacatatg
    35401 tcatagtcat atattcatct tttaaacagg ttttaactcc tcctagtata ttatcagtga
    35461 gtactttaaa tttttctttt tcatttataa taattttggt gttattattt ataatacatg
    35521 ctatatatat tataaaaatc aatataacta gtagtatttc taatgcaaaa atatatacat
    35581 cataagatat attatctgac tgaatagaca ctattttatt tggcacaaca gaaaaaacat
    35641 accaatcatt aactccaagt ggctgatagt ttaacataaa tttttcatta tctaagttat
    35701 aattaaaata tccagactta tcatttttca tattttcttt tatagttttt agacaatcaa
    35761 cttcatcaca attatctttg tcaaaaagct gaaatataca tgatattgtc ttactatttt
    35821 tatttgtttt ttgagcaatt atatctccat ttttctttgc aacaaaaaca tctgcttttt
    35881 catcaaatgc agataatccc aataattcat ttaacttttt tgaatcataa attccactta
    35941 aaacaccaac tattttccca cttttataaa ctggggtaga taaaactatt atttctttat
    36001 tattaacaat agattttaaa ggctctgata tattcttttc tccattttta gcttttttaa
    36061 aataatctct atcttgaaca ttatgagtga ttccatcatt agagtaacat tctccatcta
    36121 atgtagagat agtcattcta attaaagaac ttcgctttac atcagaatcc attaaatcaa
    36181 tcaccatttc atcatttata ttattcaagt ttcctatgaa gttagcaata gattcaagca
    36241 tgtcaaattg accactaagc cttgtcttga gattaattat attgtgttct gtaaactcag
    36301 acaaatatgt atgtgtggtt tcttttaact tattatctaa tttaaattta aacttgaaaa
    36361 cagctccaca tgttaaaata attataataa taaacaagat actaatacct aaaattcttg
    36421 gctttagtct gttcatcata ctcacatcct cattgtatat aattatatta cttcttttgc
    36481 aaacctttgt atttgtttac ttctatttct atgattcttt caatatacat ttaccttctt
    36541 cctgcattag aattaaataa gatagcttaa gatttatttc taaaatcttt attcat
//
LOCUS       DAEPXF010000018        35222 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain HB27
            SAMN13376389-rid12479423.denovo.019, whole genome shotgun sequence.
ACCESSION   DAEPXF010000018 DAEPXF010000000
VERSION     DAEPXF010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13376389
            Sequence Read Archive: SRR10518432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 35222)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35222)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001069624.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001069624.1
            Genome Coverage       :: 315x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/15/2021 01:54:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,165
            CDSs (total)                      :: 4,076
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 89
            rRNAs                             :: 10, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 10 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 19 of 69
            Pseudo Genes (incomplete)         :: 44 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 10 of 69
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35222
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13376389-rid12479423.denovo.019"
                     /strain="HB27"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="China: Hebei"
                     /collection_date="2017"
     gene            complement(149..988)
                     /locus_tag="KRL67_000811"
     CDS             complement(149..988)
                     /locus_tag="KRL67_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HBH1521716.1"
                     /translation="MSDIKNEVSKKTLSKKDVIKSWLRWFFFAQSNYNYERLQSTAFS
                     HSMLPVLKKLYPDKEELKQEVETHLSFFNTEPICGCVIHGITIAMEEEKANGADEISG
                     DGMNAIKTGLMGPLAGIGDTLTQGVITPIVLAVCIGLTEGGASIAGPILFVIAQYIIM
                     TSISFGMWTNGYKYGKKAVESILQGGIVNKVIEGASILGTLVMGGLVGRFINLSTPIS
                     YTSGDFKFSLQTDLLDKIFPGLIPLVLTLLVLFALKKGLSPIKIMFILIVVGAITGIL
                     GLF"
     gene            complement(1012..1770)
                     /locus_tag="KRL67_000812"
     CDS             complement(1012..1770)
                     /locus_tag="KRL67_000812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBH1521717.1"
                     /translation="MHISLIQAVLIAIFYYLSWSPWLTYVGFFTWNRPLLAGFVTGII
                     LGDPVQGAIIGAGINMIYLGFISAGGAQMGDPAFAGYVGTALAIASKLDVSTAMAIAV
                     PLGTVATVLWIGKMTVNSFFAHWADREVQKGNVDRVAFINIVPPQILLFAMSFIPALL
                     VVYFGPNAIDGMLEVMNENVLHVFNVIGAMLPALGIAMNLKLIGNKFTMPFFILGILM
                     AVYFKVDIIVISVIGVILALTITSIKYGKDSATS"
     gene            complement(1817..2290)
                     /locus_tag="KRL67_000813"
     CDS             complement(1817..2290)
                     /locus_tag="KRL67_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBH1521718.1"
                     /translation="MSNIALTRIDDRLIHGQVITAWCKITSAKRIVIVDDLVVKDPFI
                     VQVLQMAAPSTVKVEVHDVESGAEVLKSHNGDENLIVLVKYPKTVLGLVNSGVDLKEL
                     NVGGMGAGLGRKSFYKNISVSDEEKEIFRELISKDVKCFIQIVPDAKKIDVGTLL"
     gene            complement(2319..2735)
                     /locus_tag="KRL67_000814"
     CDS             complement(2319..2735)
                     /locus_tag="KRL67_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBH1521719.1"
                     /translation="MIHMAGIIIATHGNMAKAMLESAELIVGKQENVETLGLNYGDSI
                     DEFSRRLEDGIEKYKDEGVLVLLDFYGGTPFNTSAIIINKFAKVCELECLTGVNLPML
                     LELFLNRDTMKLKDLKNLCEEVGISGIKDVKTVLKL"
     gene            complement(2782..3834)
                     /locus_tag="KRL67_000815"
     CDS             complement(2782..3834)
                     /locus_tag="KRL67_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HBH1521720.1"
                     /translation="MKSVRFYGIRDTRVEDVDVPKILEKDDVIIKVKVAGICGSDISK
                     YSKTGPHMVGEILGHEFSGEVAQVGKEVKSFKIGDRVAVCPAMPCFECDECKKGLYSR
                     CNNVAIIGNKELGGCFAEYTKVKERNLIKIPDEISYETAAALEPVCIAGHGLFRSEAK
                     VGDTVVVLGTGPIGLFSIQWAKIFGSTKIIAVDVFDEKLDLAKELGADICINAKEKNI
                     VEEIKRLTDGDGADIVIESAGTPMTCGQVLLLTKKGGTVLYAGVPYGDVALTREQFEK
                     IVRSELTVKGTWFGNSFPFPGKEWSAGLYHMQKGDMNVEKLVTHRINLEEVPAYFEKV
                     YKRDIFFGKIMINIDN"
     gene            4230..4910
                     /locus_tag="KRL67_000816"
     CDS             4230..4910
                     /locus_tag="KRL67_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HBH1521721.1"
                     /translation="MKKVYQGKTKDVFELDNGNYLLKFKDDVTGKDGVFDPGENSVGL
                     SIDGIGRANLETSVKFFEILNNAGIKTHYVSANLEEATMEVLPAKVFGNGLEVICRYR
                     AVGSFLRRYGSCVEEGAKLDCYVEATLKDDDRCDPLITSEGLEALNIMTQAQFDSMKS
                     MTQKISNIVRDTIAEKGLELYDIKFEFGFYNDEVILIDEIASGNMRVYKDGVIVDPMD
                     LTALLLDK"
     gene            complement(5043..5363)
                     /gene="sugE"
                     /locus_tag="KRL67_000817"
     CDS             complement(5043..5363)
                     /gene="sugE"
                     /locus_tag="KRL67_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000118520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter SugE"
                     /protein_id="HBH1521722.1"
                     /translation="MKWLFLFVAGLFEVFWAFQLKNSHGFSKLTPSILTVVGMIVSFY
                     FLSLALKHLPLGTSYAIWTGIGTVGTAIVGMAILNEPISISRIACIGFIVVGIVGLKL
                     LSTN"
     gene            5745..6572
                     /locus_tag="KRL67_000818"
     CDS             5745..6572
                     /locus_tag="KRL67_000818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HBH1521723.1"
                     /translation="MNFYKIAVCQMITTENKIENINHAVDMVTEAAINGAKIVVLPEM
                     FNCPYENKYFPKFAEEYPGETTTILSKLAEKHGIYLVSGSIPELEDGKIYNTCYVFDK
                     NGALIGKHRKMHLFDIEVTGKVSFKESDTLTAGNDVTVIDTEYGKVGIAICYDIRFPE
                     LSRLMALKGAEIVILPAAFNMTTGPAHWELSIRMRALDNQIFYVGAAPARNMNASYIA
                     FGNSRISDPWGRIIAQADEKECIIYADIDRDLIPDIRQQLPLLKHRRTDLYELNTLK"
     gene            7438..8112
                     /locus_tag="KRL67_000819"
     CDS             7438..8112
                     /locus_tag="KRL67_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBH1521724.1"
                     /translation="MNNKILVVDDEEGIITLLKDYFEMNGYEVYTAQSGKDALKKISK
                     NPDIILLDINMPEIDGIEVCNRIRDYVSCPILFLTARIENTDKINGFRAGADDYIVKP
                     FDLDELGARVSAHLRREQRKSNQTKIKFYNDLTIDYEKRLICIDNNQISLSKKEFDIV
                     ELLSMNPGQVFDKEKIYDRVWGYNSNGNSDVIMEHIRKIRIKLSKHTLENYIETVWGV
                     GYKWIK"
     gene            8100..9260
                     /locus_tag="KRL67_000820"
     CDS             8100..9260
                     /locus_tag="KRL67_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HBH1521725.1"
                     /translation="MDKINNMSLKKAFFVLTLSSLIIASILTTFSYILLNNLYNSIQD
                     KYLKTPINTGIITIVQNNVNQFSDYDKTLLNIINILQLVLPLIFFIGLLLLADIIFYK
                     VKLKTPIEILNKGAMEISHNNLDFCLEYNNNDELGNLCNAFEKMRSELNKNNIKMWTM
                     INDRKQLNAAFSHDLRNPLTVLKGYSNYLTKYIPTGKLSDEKILSTTQLMSEHINRIE
                     YYVENMSNAQRLEDLVVSKSMSNINKFIENLDENISIIAKQEEKSFTLKSQINNINLF
                     FDENIILRVVENIISNAFRYARNNVSILIYLEQELLTFVIEDDGIGFSKESLKSALRP
                     FYRDKTLNDSNAHFGMGLYISKILCEKHGGSISIENNSTNSAKITAKFSTRN"
     gene            9475..10002
                     /locus_tag="KRL67_000821"
     CDS             9475..10002
                     /locus_tag="KRL67_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014119018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HBH1521726.1"
                     /translation="MKSFANNITFSRIVFALLMVSSTFYSLPFWMCYFWCGISDILDG
                     FIARKLRQQSVIGAKLDSIADFIFAVTILVTVAKNIALPMYLWWCIVAIALLRLISYS
                     IGFFKYRTFSSLHTLMNKITGASIFAFPLLYICFGLDIAGIMICIMAFLSSFEEIAIM
                     IKSKELNRDIKSIFM"
     gene            10287..10955
                     /locus_tag="KRL67_000822"
     CDS             10287..10955
                     /locus_tag="KRL67_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBH1521727.1"
                     /translation="MIIKAKQLSKIYGSNNNKVIALNNVNLEINSGEFVSIIGPSGSG
                     KSTLLHILSGLDNPTSGQVLLDDKDIYKHSEKELSALRRKSFGFVFQQFNLLPVLTAS
                     ENISMPVLLDKKQPNKEYLNEISSLLGIADRLNHLPHELSGGQQQRVAIARALIAKPD
                     IIFADEPTGNLDSKSGSEVMNLLIKTSKQFGKTLVVITHDDRIAKLADRKISIIDGVL
                     MEVK"
     gene            10958..13426
                     /locus_tag="KRL67_000823"
     CDS             10958..13426
                     /locus_tag="KRL67_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBH1521728.1"
                     /translation="MKNYLSLSIKELKSQKLMTTFIIIAIVLSTIMTTVVGQSIGILQ
                     NLRIEQARSFNGDRHVSFHQLTKNQVDDIKKDDRVYQAGTSITLGSSKIKDSGISVLV
                     KEYDKTGLSNYPKLMKLKSGNLPKDKNEIALDENTLKLMGIKPRLGVTIPMNLDISLL
                     NDTIPPYNYTANFKLSGILEDDYTGYVSGIVNGIVGKGASENLLPKRYILSSLDFKIK
                     QQEKFQEIVNQLAKKINLSHNSIQYNWIFLNALKIQFEKDENSSNSDGISMIILVSLF
                     VALLVLLASGLVIYNILKISVTKKIKEYGCLRAIGAEPNQIYKIVILQILILCTVAIP
                     IGAVIGIISSKGITGMVTNILNPDILLANDNKEITELIHKNTTAYMFPLVLSTSISLI
                     FSLISALPSAIYASHVSPKIAMAGSTTKIKRKIKREKTIKNFERHLAWLNLKRNKGRT
                     IITILSLFMSITVFVALSEFSNVLDVSRSVSNLKEGDFSLTNEISGFDKPILDKIDNM
                     KNVNRTSFIKYSEYKQGEIDTDINFENSGEMLKIIGIDEQTLKDLMPSITDSTLEDFK
                     NGSICFIKNPIAISTPGVKTKYTNLKPKDNITINKKQLDIYSTVDKMFFLQGNGWVNG
                     VDVIVYDSVYNTLTNKNKFNQINIYAKDKSDLEQIRLSIEQICENNPGSHWISYIESD
                     KQLKESFKQIEFLAWAVILFVGLIGTLNIINTTHTNINTRTNEIGVKRAIGMSNSSLY
                     KMFLWEGVYYGIFAAIFGSIAGYVSAIIINMATIEKLDFTNIPITSILQATIISILAC
                     IIATLIPLRKVKKMNIIDCIDINL"
     gene            complement(13666..15090)
                     /locus_tag="KRL67_000824"
     CDS             complement(13666..15090)
                     /locus_tag="KRL67_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing protein"
                     /protein_id="HBH1521729.1"
                     /translation="MKNQSLIAQFRRTFIFIIIASIVATVITYVFAIYLYIYSLNKDI
                     YPPNYYERQVPRIEKYINEKNIALLSQSNEEGLKRTIRGDDMLYQVVDNNGNILYGTN
                     PKKTFKTKEELFNNFINKTVRKGGYIYTVPIKGDNGKIEGAVILFYQVKITFANSRGR
                     FVFAVIIMALFSPFLYIVGFTRWLSKRFVKNINQPLHLLIDASNKIKEKDLDFEIDYY
                     SDNELGKLCSAFSEMKDELKGSLSAQWKMEQERVEMVEALAHDLKSPLSIILGYTDAL
                     IGNNTDDNEKLHRYLTVIRENTEKSAALVQKMQYTSDLEKSNIQLNLVPINLPEFLRQ
                     KVQDYELQAHQKEVELILKMQGNIQSPIQIDVDRLTRIFDNIISNSLQYTPSGGNISI
                     TVKDEKNRISYEICDSGSGFSSKDLKKALDKFYRGDEARQTKGGHSGLGLYIVKQLVE
                     QLGGSVKIENSKSGGACVKFWHSI"
     gene            complement(15109..15801)
                     /locus_tag="KRL67_000825"
     CDS             complement(15109..15801)
                     /locus_tag="KRL67_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBH1521730.1"
                     /translation="MSKILIIDDEMDLVMLLEDELKAKGHEVLIAYDGQAGIELSKQE
                     PDLIILDIMMPKMNGFEVCQAIRDDVLCPIIFLSAKQSETDKIKGLTFGGDDYVTKPF
                     GLRELMARIEANLRREKRSQYINEENKRSTLYFGKLCLYMKERAIKINGKNIDLTKIE
                     YDIVELLALHAGQVFSREQIYEKVWGCDSEGDSSTVVERVKKIRAKFSAVTPEKEYIS
                     TVWGIGYKWNKM"
     gene            complement(15853..16632)
                     /locus_tag="KRL67_000826"
     CDS             complement(15853..16632)
                     /locus_tag="KRL67_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lantibiotic immunity ABC transporter MutG family
                     permease subunit"
                     /protein_id="HBH1521731.1"
                     /translation="MIKLLSSEWLRAKRTAVQWLTLCMPIIFSLCVVFYLTTKSGSTQ
                     EFAFEGFFTVWTVFIIPIGVGILAGFIVQEEELAGNFNGFLCVGISRVRLYLGKFLFL
                     LFCLTICTFIATLILCIGMSIAVPSGATIWLFLSAAGLVVIGTLPLLAIHLWVSFAWG
                     MGASIGISIGGILMAAILGVTSVGEKVWAFVPWTWPVKLGMLPGIYFIKEAGTISTEV
                     FYSKVMQTASIELIVVAIGLIIFLIGGVIWFKMWEGRKSYE"
     gene            complement(16629..17399)
                     /locus_tag="KRL67_000827"
     CDS             complement(16629..17399)
                     /locus_tag="KRL67_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lantibiotic immunity ABC transporter MutE/EpiE
                     family permease subunit"
                     /protein_id="HBH1521732.1"
                     /translation="MNALQSELLKYKRTFMGKLIVFFPVSFAAYAFIMQSTLMQNPLS
                     QTTSWAWQNLLALIFNWWSFLFLPIGFALFATLVAFQEKKAGNYRALRTHNVSPMTLW
                     INKVIAMAVYSFISTLVLIFVTIITGLILKAGPVPFGQIIGASIVCWVVSLAILPLQL
                     WLATWKGMFLSMGVGALGMIFGVLAATKPFWIAVPWSWAVRMVCPIINIHPNGTVLEA
                     GDPLLNTSVIPIGIVVSLVVFIVLTALTAAWFNRRDDK"
     gene            complement(17396..18100)
                     /locus_tag="KRL67_000828"
     CDS             complement(17396..18100)
                     /locus_tag="KRL67_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014466355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lantibiotic protection ABC transporter
                     ATP-binding protein"
                     /protein_id="HBH1521733.1"
                     /translation="MKDLILETKNLSKRYGEQMAVNNVSLQIERNSIYGLLGPNGAGK
                     STTLKMLVGLLRPTDGRIIFDRKPWKRESLAKIGSLIESPALYGNLTAEENLLVHTKI
                     LGIPKDKIYEVLEIVNLKNTGKKRASQFSMGMKQRLGIAIALLSDPELLILDEPTNGL
                     DPFGIQELRELIASFPKKGITVILSSHILSEVAQVVDNIGIINGGRLLFQGMPDSNEN
                     LEEFFTDVILKGGQNR"
     gene            complement(18336..18848)
                     /locus_tag="KRL67_000829"
     CDS             complement(18336..18848)
                     /locus_tag="KRL67_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH1521734.1"
                     /translation="MKKISQEKISTHIQEVPQEKIEQWKSVYEKYKSILKVNSKSVLE
                     IIEFLKRKYVIVEETSEEFKHVVINNIKLNKPFSQKIPSDKELKSIVFTIQNEGNAIK
                     LYDTQDETYSGCKIIVGMEFETGCVFVDGSDELNDEITAFKGLDKYDINNYYLVANYI
                     RCLKKYKLID"
     gene            19174..19431
                     /locus_tag="KRL67_000830"
     CDS             19174..19431
                     /locus_tag="KRL67_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH1521735.1"
                     /translation="MILNRTELFRGYSLNRFNEICNILSVNKIKYKYKVKNNAYSKKP
                     FFNEITLGNLGQKEDFSYEYLIYVHKGDYEQAEALINSKLR"
     gene            19574..20200
                     /locus_tag="KRL67_000831"
     CDS             19574..20200
                     /locus_tag="KRL67_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase family protein"
                     /protein_id="HBH1521736.1"
                     /translation="MKINIKSTLNEFIKQNNLFDDNRIISYLPNITIDTDKIKTIMIN
                     EVVPTNPNDDFYSIDKDADYLKTTIPLFNNAGIVVSNINDILDMGIYITNAVKLPKSE
                     YTITRDTIKLHMPILEEEIKLFKNLEVVMLMGDVAKKSFNMITKKHIKKNVIPSISTY
                     KLRNNELYYDNLRVFPSYIMTGGNILIEKSKFEMASEDIKKMFEIING"
     gene            complement(20450..21229)
                     /locus_tag="KRL67_000832"
     CDS             complement(20450..21229)
                     /locus_tag="KRL67_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901940.1"
                     /note="N-terminal region resembles flavodoxins. C-terminal
                     ferrodoxin region binds two 4Fe-4S clusters; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EFR1 family ferrodoxin"
                     /protein_id="HBH1521737.1"
                     /translation="MNSKIYYFSGTGNSLVVSKTLKEMLNESKEVIPLAIHRKENNID
                     INEDILVIVFPVYFADVPDIVKSFVEKLNFNINTHIYAIATCNGVAGHSLFTIDKLLK
                     KKEKKLSAGFLINMPGNALITPPDVEEERLRNQTNRVAEIAKCINNLEIGNIEGKNNL
                     KSHIDSVVLRLVGKNMQIATTKFFAENKCNGCGTCKRVCPVENISIVDKKPKWGNECE
                     RCLACFHWCPKEAINVKKSFLTDRKKYHHPDITVKDIFLDK"
     gene            complement(21297..21758)
                     /locus_tag="KRL67_000833"
     CDS             complement(21297..21758)
                     /locus_tag="KRL67_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HBH1521738.1"
                     /translation="MGGARDYLNGGKMKDFEKLYEATTKLVSTSLKVKDDLKKMFKQN
                     GYDITTDHYALLRFLWEQDGISQIDLCEKSCKDKSNTTRILDVMKNKGLIVRKVDVKD
                     RRKFQIFLTDLGRELEEPLNKIANVYATETFKSISDEELPLFTDILDRIGK"
     gene            complement(21734..22411)
                     /locus_tag="KRL67_000834"
     CDS             complement(21734..22411)
                     /locus_tag="KRL67_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HBH1521739.1"
                     /translation="MIITVINGSPRKNGATSKVLTYLYKDIERLIPDVKINYFDLSEV
                     NPSYCIGCLNCYKMGKCINQNDKVEYIHDIITKSDGVIFGSPTYGSSVTGLFKVFTDR
                     AHMMLERLLYRKPCIAVTTYENARGSKAISFIKSMVLDSGGYVCGSLSIKTGFNQNPI
                     TEKVESKIQKVSKKFIYCIEEKKNPPVFSQIYNFIAINAVLKPMAFKDIEQYKGIIDR
                     WEAQGII"
     gene            22645..23031
                     /locus_tag="KRL67_000835"
     CDS             22645..23031
                     /locus_tag="KRL67_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HBH1521740.1"
                     /translation="MKKLPHISEAEYQVMKIIWKYAPISTTEVIEKLVETSTWSPKTI
                     QTMLLRLVKKGALTYEKNSRVFVYTPLVKEEEYIATESSSFLNRFYNGTLNSMVLNFL
                     ENDKLSEDDIEELREILNKRTTKGDK"
     gene            23034..24833
                     /locus_tag="KRL67_000836"
     CDS             23034..24833
                     /locus_tag="KRL67_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaR1 family beta-lactam sensor/signal
                     transducer"
                     /protein_id="HBH1521741.1"
                     /translation="MYAPFFIRFLLSTLTLSILIVVILLAKKVFKKHISAKYQYNIGF
                     ILLIMLILPFVPLKYINLGNVFNYLYTFNQLGSNTNRTSMIKNSPNSIINNSNALNDF
                     SVSLNQVNFDSLNILLLISWIIGILIMIAITIHCNIKIKNMKKSIQILRDEEIVFTFE
                     KCKKDIGINKDFILCKSPIINTPITFGYFKPHIILPDKSISKLSLKDIKYILLHELQH
                     FKNRDILINYIMCFFQILYWFNPLVWYAFKEMRIDREIACDISVLKKLDKDSYIEYGQ
                     TLINFVDNISRPSNLTLTSDIGGSKKQIKKRLLKIMDFHIESSALKFKSILIFTLVAL
                     IVFGNIPRLSIMADINEKYRPTNDKTVYEDLSQYFKGFDGSFVMYDLKSKQYHIYNKE
                     KSKTRISPNSTYKIYSALFGLENGIITRDNSHIPWDGKHHSNSSWNEDQDLFSAMENS
                     VNWYFQTLDKKTKLKNIQTYLKQIGYGNHDVSGGISNFWLESSLKISPIEQVKLLESF
                     YTNKFKFEDKNIEAVKDSLLISKKDGVSLFGKTGTANINGKDVNGWFIGYVEKNENTY
                     FFATNIQSKDYSNGSSASKITLSILNDKKIYNP"
     gene            complement(25186..27273)
                     /locus_tag="KRL67_000837"
     CDS             complement(25186..27273)
                     /locus_tag="KRL67_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HBH1521742.1"
                     /translation="MIKVVYMRKNKKTKKMIIFGFLMCLLIVIIVFLAKFILDGLNKN
                     KPDEVFKQYMSFANKKQYEKMYDLLDEKSKSENKKEDFVLRNKKIYEGIDAHDISIKI
                     NDIKKNKNNDKVINYDSKMDTLAGEISFSNEVLLTRDRQKNYKIKWQSNVIFPELEED
                     NTVRVSKLKGKRGRILDRNGVMIAGQGLASMIGLVPGKMSDNIEDLKKLSTLLNVSVE
                     QIEKKLNASWVKENSMVPIKTIEKIKENADGTVKEEDKELQESLLSIPGVKISNTEVR
                     VYPFGEKTGHLTGYVQNVNAEILKEKEGKRYNDNSIIGKIGLENLLEDRIRGIDGYEI
                     IIADRYGDKKETLVTEPKVDGEDVKLTIDSKLQSKLYDQMKNDKGCAVVMNPKTGEVL
                     SLVSSPSYNPNEFILGMSEDRWNELNKNENKPMYNRFKARLCPGSSFKPVTAGIGITT
                     GKINPNENFGHSSLSWQKDSSWGSYKVTTLKDYGNTANMKNALIYSDNIYFAKAALKI
                     GEDVLAKELLKLGFDESMPFEFGLSSSKFGTDNKFETEIQLADTGYGQGKLLVNPVHT
                     ASIYSAFVNDGDMIKPHLEFKKAEFWKKGVFSKEAVKIIRDDLLQVVESPDGTGHSAM
                     IKGISIAGKTGTAEIKASKDDVTGTELGWFNAFTVDENSDKQYLIIAMIEDVKSRGGS
                     HYVIPIVKSVFED"
     gene            27634..28212
                     /locus_tag="KRL67_000838"
     CDS             27634..28212
                     /locus_tag="KRL67_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBH1521743.1"
                     /translation="MPTKGEQTKQFIIDTSIMLFSEKGYSNVTMKDICEACNLSRGGL
                     YRHFNSPKEIFISILNTHKYYSQNLLDKGIKENISAKHMLEYFFQQQKADINGKYCGF
                     DFAIHEFAFVEKDQRDYMNKRYNSAITMLSNLIDYGQSKSEFKKCDSKSVAIHIMFLF
                     DSMKTSASILKLTNKDIDNQINIIKDMVILYE"
     gene            28205..28864
                     /locus_tag="KRL67_000839"
     CDS             28205..28864
                     /locus_tag="KRL67_000839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chloramphenicol acetyltransferase CAT"
                     /protein_id="HBH1521744.1"
                     /translation="MNKQKFHKIDLQNWERYTHFYYFSKMTNVGFNLTIEIDITNLLK
                     ETKKRNIKFYPAYLYTITTCLNRYRELKIAYDEDELGYWNTLTPLYAIFHDDNKTFSS
                     IWTEYDENFSIFYENYLADVKEYGKNHGFLAKPNPPRNCYTVSCLPWISFKSFTVHNH
                     GLENYFFPTIEAGKYYQKDEKILLPLSITVHHATTDGYHVSCFIDYIQKMCFNTKIWL
                     H"
     gene            complement(29076..30950)
                     /locus_tag="KRL67_000840"
     CDS             complement(29076..30950)
                     /locus_tag="KRL67_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HBH1521745.1"
                     /translation="MNKYNKIANELIEIIGENNIISITHCATRLRVMVKDREIINDKK
                     VEKVNEVKGVFFTSGQYQIILGTGIVNKVYAEVEKMGLKTLSKKEQDELVKNNETGFK
                     KVMRTLADIFVPIIPVIAATGLFLGLKGCLFNDNVLGLFGMSSANIPLYIQTLVSVLT
                     ETAFAFLPAIIVWSAFKVFGGTPVIGLVIGLMLVSPILPNAYSVADPSNEVEAIMAFG
                     FIPIVGCQGSVLTAIVTAFIGANLEKWFRKHMPNVLDLIFTPFFVMLITMLVILLGVG
                     PIMHTIELKMVDIISLLIGLPLGIGGFIIGFTYPLAVITGLHHTYVMIETSLLANTGF
                     NALITLCAMYGFANIGTCLAFMKKSKNNQVKQTAVGAMLSQLFGISEPVLFGIQLRYN
                     LKPLIIMCASSGLGAAILSILHIQSNSYGLAVLPSYLMYIYDGYNLITYLLVSIFVVV
                     FCFIATCLFGVPKEAINEDEDEELVFNENNENFVSPAKGKIVALENVPDETFSKKMLG
                     DGFAIDIIDGKIVSPISGKLETVFSSGHAFGIKGTNGEVLIHVGIDTVELNGDGFDVA
                     VKQGDMVKQGDVLVNVDLKRIHELGKSTLTMVLFPDGKKVNILDINKDVKIGQRICVE
                     "
     gene            complement(30955..32619)
                     /locus_tag="KRL67_000841"
     CDS             complement(30955..32619)
                     /locus_tag="KRL67_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HBH1521746.1"
                     /translation="MEKKWWQKEIVYQIYPRSFQDSNNDGIGDLQGIVSRLDYLKDLG
                     ITSLWLCPIFKSPMDDNGYDISDYLDIASEFGSMEDLKELIKESKDRGIKIILDLVLN
                     HTSDEHPWFQEALKNPESPYHDYYIFKKGNDLPNNWRSVFGGSVWEKVENRDEYYFHS
                     FGKKQPDLNWENPILRSELYQMINTWSQMGVAGFRVDAITFIKKNLSFQSLESDGVDG
                     LVKCTKAVRNQPGIEKMLYELKENTFKKYDCMTVAEAAGISYDQLGEFIGEDGFFSMV
                     FDFKHADLDVASGSEWFKRISWTISDLRKYILDSQMYVQQHGWAANFIENHDQPRATT
                     KYLLQYERNDDAVKMLGAMYFFLRGTPFIYQGQELGMTNFKRNTINDFNDLSSIDQYY
                     RSIDEGYSEEEALEFVNLRSRDNARTPFPWSDKENAGFSDTTPWLKVIENYKDINAQS
                     QINNSESILEFYKKMIKLRQDSEYSDCLIYGSIEPIDIESDNVIAYRRQLDLVVIDCY
                     YNFSDKPVEIKTEEKYDIVFQNLDDINLLNKTVSLKPFQAVLLKVR"
     gene            complement(32633..33454)
                     /locus_tag="KRL67_000842"
     CDS             complement(32633..33454)
                     /locus_tag="KRL67_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HBH1521747.1"
                     /translation="MKLYKLLILDIDGTLRDEVDGIPESAKHAIRLCQKNHCSVVICT
                     GRSMGTIQDDVLSLGVDGYIAGGGNYIQYHGELLYNQSFNQRLIKKVVCLLKKREVAF
                     SIESREKVFMNQKAKEIFETMNQLKGTNSCINKQHIQEKITYENNIEEYKSQDIHKIC
                     LWSNEKVFDEVKDILQDKMELAQRDISSQYYEIIQKDFHKGKAIQRLQERLGITQKET
                     ICFGDGQNDIVMFQASDITIAMKNSHQQLKDIATSICEDIFDNGIYKELKRRNII"
     gene            complement(33553..34317)
                     /locus_tag="KRL67_000843"
     CDS             complement(33553..34317)
                     /locus_tag="KRL67_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HBH1521748.1"
                     /translation="MKIEGLFNKYYNSFTANDKYICECILNHKEDCIKLSIDEFADKY
                     HISTSALSRFAQKLQLPGYSELRVIIRLNETEVEYKNQSKHQLMDCYNKVIDDIEKKD
                     CSIMFERMKKANRIIVFGEGYSQERVAKEMKRIFLPTGKIIYDVYGYDMIQALNHFIK
                     TDDMIFFISLNGDASTIIDFAKKMKMKGIYMVSITKMISNPLARLCDENLYIQSISIS
                     VDKQLDYDVTTPYFILIELLYIKYKMYLESEHIPRK"
     gene            complement(34314..35063)
                     /locus_tag="KRL67_000844"
     CDS             complement(34314..35063)
                     /locus_tag="KRL67_000844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HBH1521749.1"
                     /translation="MRLESLVNQNYDNLNENDKIIIKQILICQREYRNFSCEKIANEC
                     NVSRATLLRLCRKIGLDSFSDLKYLLKNNQLEFVNNDNNFHDVCENYRLLMNELQKIS
                     FQDICKIIYDAETIYIYGTGNEQKSLAQEIKRIFLSVGKCVIDLFDIGEIEFMRESFQ
                     KSDLFIIISLSGETKAGIEIAKFINNHIHTISLTRLDNNTIATLCEYNLYVETQKLDT
                     LQAISYEVVGAFYALLDLLHMNYIEYRRRKE"
BASE COUNT        12429 a         5864 c         4229 g        12700 t
ORIGIN      
        1 ttaaatacta catatagctt acataagttt aaaatttaat ctattataaa attaccctaa
       61 ttgccaaact ttttaatata aattttccaa catcctacaa atataatcaa tagatataga
      121 atcctaactt acaccaatat atatttattt aaaatagtcc taaaatacct gtaatagctc
      181 ccactactat cagtataaac attattttta ttggagaaag accttttttt aaagcaaaga
      241 gtactaataa agtaagtacc aaaggaatta atcctggaaa tatcttatca agtaaatctg
      301 tttgaagact aaatttaaaa tcaccactag tataacttat tggtgtagat aaattaataa
      361 acctacctac aagtcccccc atgactaaag tacctaatat agaagcccct tctataacct
      421 tatttactat tccaccttga agtatacttt caacagcttt tttcccatac ttatatccat
      481 ttgtccacat tccaaaagaa attgaagtca ttattatata ttgagctatt acaaataata
      541 tagggcctgc aattgatgct cctccctcag ttaaaccaat acaaacagct agcactatag
      601 gagttataac tccttgagtt aaagtatcac caattcctgc tagtggaccc atcaagcctg
      661 ttttgatagc attcatacca tctccactta tttcatctgc tccattagct ttttcttctt
      721 ccatagctat tgttattcca tgtataacac atccacatat aggttctgtg ttaaaaaatg
      781 atagatgagt ctccacttct tgttttaact cttccttatc tggatataat tttttaagta
      841 ctggcaacat tgaatgtgaa aaagctgttg attgcaatct ttcataattg taattagatt
      901 gtgcaaagaa aaaccatctt aaccatgatt ttattacatc tttcttgctt aacgttttct
      961 tagatacttc attttttata tctgacataa tattttcccc ctcacatgta tttaagatgt
     1021 agcactatct tttccgtact taattgaagt aattgtaaga gcaagtatta ctccaataac
     1081 agatattact attatatcta ctttaaaata aacagccatt agtattccta atatgaaaaa
     1141 gggcatagta aacttgtttc caatcaattt taaattcata gcaatcccaa gtgctggcaa
     1201 catagcccca attacattaa atacatgtaa cacattctca ttcattactt ccaacatacc
     1261 atctatcgca tttggaccaa aataaacaac taataatgct ggtataaaac tcattgcaaa
     1321 taacagtatc tgtggtggta ctatatttat aaatgctact ctatcaacat tccctttttg
     1381 aacctctcta tcagcccaat gtgcaaaaaa agaatttact gtcatctttc caatccaaag
     1441 tacagttgca acagttccca atggtacagc tattgccatt gcagtactta catctagctt
     1501 agatgctata gctaaagcag tacctacata tcctgcaaaa gctgggtctc ccatttgagc
     1561 tccccctgct gatataaaac ctagatatat catatttata cctgctccta taattgctcc
     1621 ttgaacaggg tctcctaata taattcccgt tacaaatcca gccaatagag gtctattcca
     1681 tgtaaaaaat ccaacataag tcaaccacgg actccaagaa agataataaa aaattgctat
     1741 taatactgct tgaattagtg aaatgtgcat attcttcctc cctattttct tttaaagatt
     1801 tacaagatag tcttctttat aataatgttc ctacatcaat tttctttgca tctggtacta
     1861 tttgtataaa acatttaaca tcttttgata ttagctctct aaatatttct ttttcttcat
     1921 ctgacacaga tatattttta taaaaacttt ttctaccaag cccagctccc atacctccaa
     1981 cattcaattc ctttaaatct accccactat ttactaaacc taagacagtt ttaggatatt
     2041 tgaccaatac tattaaattt tcatctccat tatgtgattt taaaacttca gcgcctgatt
     2101 ctacatcatg tacttcaacc ttaacagttg atggagctgc catttgcaaa acttgaacta
     2161 taaatgggtc ctttactact aaatcatcca cgattactat cctttttgct gatgtaatct
     2221 tacaccaagc agtaataact tgcccatgta ttaatctatc atcaattctt gttaatgcta
     2281 tattactcat aataattcct ccaaattttt aatttatttt acaactttaa aacagtttta
     2341 acatctttga ttcctgatat cccaacttcc tcacataaat tcttcaagtc ttttaacttc
     2401 atagtatctc tgtttaaaaa taactctaaa agcataggta aatttacacc tgtcaaacat
     2461 tctaactcac ataccttagc aaatttattt attataatag cagatgtatt aaatggagtg
     2521 ccgccataaa aatcaagcaa tactaaaacc ccttcatcct tatatttctc aattccatct
     2581 tctaaccttc tactaaactc atctatgctg tcaccatagt ttaatcctaa agtttctaca
     2641 ttttcctgtt ttcccactat taattcagca ctttctaaca ttgctttagc catatttcca
     2701 tgtgtggcta taattattcc tgccatatga atcatacctt tcttaacctt ttttatatgg
     2761 attattattt agtactctaa tctaattatc tatattaatc attatcttac caaagaaaat
     2821 atcccttttg tatacttttt caaaataagc tggtacttct tctaaattta ttctatgtgt
     2881 tactaatttt tcaacattca tatcaccctt ttgcatatga tacaatccag cactccattc
     2941 ttttcctggg aatggaaatg agtttccaaa ccaagttccc tttacagtta attcacttct
     3001 tactatcttt tcaaattgct ctctagtcaa agctacatcc ccatatggta cacctgcata
     3061 caatactgtc cctccctttt tagttagcaa cagtacttga ccacatgtca ttggagtacc
     3121 agccgattca ataacaatat ctgcaccatc tccatcagtt aaccttttta tttcttctac
     3181 aatattcttt tcttttgcat ttatacaaat atctgcccct aactcttttg ccaagtctaa
     3241 cttctcatca aatacatcta cagctattat ttttgtagaa ccaaatatct ttgcccattg
     3301 tattgaaaat aatcctattg ggcctgtacc aagtactaca acagtatctc caactttagc
     3361 ttctgaccta aaaagaccat gtcctgctat acatactggt tctagtgcag cggcagtctc
     3421 ataacttatc tcatctggta tttttattaa atttctctct ttcacctttg tatactctgc
     3481 aaaacaacct ccaagttctt tatttcctat tattgctaca ttattacacc ttgaatacaa
     3541 tcctttttta cattcatcac attcaaaaca tggcatggct ggacaaacag caactctatc
     3601 tcctatttta aaactcttta cttctttacc aacctgtgcc acttctccag aaaattcatg
     3661 tcctaaaatt tctccaacca tatgtggacc tgtctttgaa tattttgata tatcagaacc
     3721 acatattcca gctactttta ctttaattat tacatcatct ttttctaata tttttggtac
     3781 atccacatcc tccactcttg tgtctcttat accataaaat ctaactgact tcactattaa
     3841 ccacccctac tttttatttg agaatatata cttattgttt atatgttaaa tatctaaagc
     3901 aactttcatg ccaatatatg taagtattgt attcatgcat tttataaaac tatcattaaa
     3961 tgtgtgttaa agtgtgtcaa aagtttctta tattaaaact tatgtgtgtc aatataaacg
     4021 acactaacct tttatactag ttctcttatt ataagtttta taaaatgtac tttttttgtt
     4081 atttttataa aattgttcgg aaattcccct tctatttgtg atataatatt aaatataagg
     4141 tatcaatata aattttcatg cgaatatcag aacttttttg acactaatgc cttattcaat
     4201 ataataattt aactttttgg aggttgttta tgaaaaaagt gtatcaagga aagactaaag
     4261 atgtttttga attagataat ggaaattact tacttaaatt taaagacgat gtaactggta
     4321 aagatggagt atttgaccca ggcgaaaata gtgtaggtct aagtatagat ggaattggta
     4381 gagctaattt agagacgtct gttaagttct ttgaaattct aaacaatgct ggaattaaaa
     4441 ctcattatgt aagtgctaat ttagaagaag caactatgga agttctacca gcaaaagttt
     4501 ttggtaatgg attagaagtt atttgtcgtt atagagctgt tggtagtttc ttacgtcgtt
     4561 atggttcatg tgttgaagaa ggtgctaaac tagattgtta cgtagaagca actttaaaag
     4621 atgacgaccg ttgtgaccct cttataacat ctgaaggttt agaagcttta aacattatga
     4681 ctcaagctca atttgattca atgaaaagca tgactcaaaa aatttctaac atagttcgtg
     4741 atactatagc tgaaaaaggt cttgaacttt atgatatcaa atttgaattt ggattctaca
     4801 atgatgaagt aatcttaata gatgaaatag catctggaaa tatgcgtgtt tataaagatg
     4861 gtgtaatagt tgaccctatg gatttaactg ctcttttatt agataaatag catatacata
     4921 taattttcaa atataataaa cttataaata agataagcta tgaagtaaag actttttaaa
     4981 tgttgattat ttttaaacat tttctcgcct ttaatttcat agcttattta aattaatata
     5041 ccctaattag tagataatag ctttaaccct acaattccca ccacaataaa gcctatacat
     5101 gcaattcttg atatactaat aggctcattt aagatagcca tgcctacaat agctgtacca
     5161 acagtaccaa ttccagtcca tattgcatat gaagttccaa gtggaagatg ttttagtgct
     5221 aatgacaaaa aataaaaact tacaatcatt ccaacaacag taagtatact tggagttaat
     5281 ttagaaaatc catgtgaatt ttttagttga aaagcccaaa acacttcaaa caaacctgca
     5341 acaaacaaaa ataaccattt cataaactca tccctctttt caaatatatc tcttattttt
     5401 ccaataaaat aacctgagaa atattataaa atatctccca ggtttttgtc cttccgtgtt
     5461 cacaacaaaa actatgttgt gggttttatc ttggaccaga ccaatcatta ataattgcgg
     5521 aaccctataa aactctatca attattctat tttatctata ataatactat caacatatat
     5581 aaaaaaagtc aatatatggg ttccatctat aataaaaata acttttatat acctcttaat
     5641 attcttattc acttctctcc atacccattt aggtaaatct aagaatatct aatttttatt
     5701 taatatgtta taataacata aaaatattga aaggagtaat ttctatgaat ttctataaaa
     5761 ttgcagtatg tcagatgatt actacagaaa ataaaattga aaatatcaat catgctgtag
     5821 atatggtaac agaagctgcc atcaatggag ctaaaattgt agttttgcca gaaatgttta
     5881 actgcccata tgaaaataaa tattttccta aatttgctga agaatatcca ggtgaaacaa
     5941 caacgatatt aagtaaatta gctgagaaac atggaattta tctagtttct ggtagtattc
     6001 ctgagttaga agatggaaaa atctataata cttgctatgt atttgataaa aatggagctc
     6061 taattggaaa acatcgtaaa atgcacttat ttgacattga agttacagga aaagttagtt
     6121 ttaaagaatc agatactctt acagctggaa atgatgtcac tgtaatagat accgaatatg
     6181 gaaaagttgg tatagctatt tgctatgata ttcgtttccc tgagttatca cgtttaatgg
     6241 ctcttaaagg tgctgaaatc gttatcttac ctgctgcatt taatatgact actggtcccg
     6301 cccattggga gttatctata agaatgcgtg ctttagataa tcagatattc tatgttggtg
     6361 ctgcacctgc tagaaatatg aatgcttctt atatagcttt tggaaattca agaatttcag
     6421 acccttgggg aagaataatt gctcaagctg atgaaaaaga atgtattatt tatgctgaca
     6481 ttgatagaga tttaattcct gacataagac aacaattacc acttcttaag cacagaagaa
     6541 ctgatttata tgaacttaat actttgaaat aaaacagaac ataaccccat aaatcataaa
     6601 ttaagtgtta aaaaaattta tggggttctc attaatttga tttaaaatta atttcataaa
     6661 aataatgcta aaattaattt aattaaataa tactaataat tactaatgat tatgctaatt
     6721 atcagcattt actaatttaa tcactattag ttatttcttt tattaaatat ctttactttt
     6781 ttacttatta tgtcttctag ctttacattt ggattatggt atttctttct atttttagtc
     6841 acatctttct attgaattac ttgcttaaaa caccaatgca cacatgccag ataatgttca
     6901 caattatgta gaaatcttgc tctaccttct tcaatgttga tattatctct cctagttgat
     6961 attatctttc ctaaaatttc ttttaattaa aaaacaacat tagttgatat atcaagtata
     7021 ttttaagcaa ctgaaaaaac aataataatt gatatatcaa gtatatttta agcaactgaa
     7081 aaaacaataa taattgatat atcaactaat taatgtagat taattcattt ttataattta
     7141 tatacttttt aattaatcta ccttatttaa ttaaaatcct tcctttctat ttatccattt
     7201 tcaacttaaa ttataagatt tcatcagcta tatttttgca taccaataag tattaaatat
     7261 caaatatata ttctattaaa atttctaata atttcatcct tgatttattt aatatataaa
     7321 aatatactaa ctttaatgat taagttttga aatataacta agaatttgat aatttattga
     7381 gatttttata ctaaaatata catactatac aaacttaatt tagaagggag cattcttatg
     7441 aacaataaaa tactcgtcgt agatgatgag gaaggtataa taacactctt aaaagattac
     7501 tttgaaatga atggttatga ggtttacact gctcaaagtg gaaaagacgc attaaaaaaa
     7561 atatccaaaa atccagatat cattctactt gatataaaca tgcctgaaat agatggaatt
     7621 gaagtttgta atagaattag agattatgta tcatgcccta ttctatttct tacagctaga
     7681 attgaaaata ctgacaagat aaatggtttt cgtgctggtg ctgatgatta tattgtaaaa
     7741 ccttttgatt tagacgaact tggagctaga gtatctgccc atttaagacg tgaacaaagg
     7801 aagtcaaatc aaactaaaat taaattttat aatgatttaa cgatagatta cgaaaaaagg
     7861 ttaatttgta tagataataa tcagatttca ctttcaaaaa aggaatttga tatagtagag
     7921 cttctctcta tgaatcctgg acaagtattt gataaagaaa aaatctatga tagagtctgg
     7981 ggatacaata gcaatggaaa tagtgatgtt ataatggaac acattagaaa gatacgaatc
     8041 aaactatcaa aacatactct tgaaaattat atagaaacag tctggggagt tggttataaa
     8101 tggataaaat aaataatatg tcattaaaaa aagctttctt tgttcttact ttatcttcac
     8161 taattatagc atcaatactt actactttct catacatttt gcttaataat ctatataatt
     8221 ctatacaaga taagtattta aaaacaccta taaatacggg tattataacc atagttcaaa
     8281 acaatgtgaa ccaatttagt gattatgata aaacattact taatataatc aacatacttc
     8341 agcttgtact tccacttata ttttttatag gacttttatt attagctgat ataatttttt
     8401 acaaggtaaa actaaaaaca cctatagaaa tactcaataa aggtgcaatg gaaatatccc
     8461 acaacaattt agacttttgt ttagaatata ataataatga tgaattaggt aatctatgta
     8521 atgcttttga aaaaatgagg tctgaactaa ataaaaacaa cataaagatg tggactatga
     8581 ttaatgatag aaaacaactt aatgctgctt tttctcatga tttaagaaat cccctaacag
     8641 ttctaaaagg ttactcaaac tatttaacaa aatacatacc aacaggtaag cttagtgatg
     8701 agaagatttt atcaaccaca caacttatgt ctgagcatat taacagaatt gaatactatg
     8761 tagaaaatat gagtaatgct caacgattag aagatttagt tgtttcaaaa tctatgtcga
     8821 atataaacaa gtttatagaa aacttagatg aaaatatatc tatcattgct aaacaagaag
     8881 aaaaatcttt tacacttaaa agtcaaataa ataatataaa tctatttttt gatgaaaata
     8941 tcattcttag agtcgtcgaa aatataatat cgaatgcttt tagatatgct agaaataatg
     9001 tatccatact tatatatctt gaacaagaac tccttacctt tgtaattgaa gatgatggta
     9061 taggatttag taaagaatct ctaaaatcag ctttaagacc tttttataga gataagactt
     9121 taaatgacag caatgcacac tttggtatgg gtctttatat ttcaaaaata ttatgtgaaa
     9181 aacatggtgg ctctatttct attgaaaata attcgactaa cagtgctaaa attactgcta
     9241 aattctcaac aagaaattaa aatgattttg taagctttca ctgctcttat ataagttttg
     9301 ttggtttaga atttataaaa tgtatttcaa tttacaacca atatatacta ttttgtagaa
     9361 gttgtaagag ttgcttcaat tataatactt tggtaaaagg tattttaaac aataatagaa
     9421 atatatattt aagtatgtag aatagtacga aattagaatt ttggaggtga ttatattaaa
     9481 agttttgcta ataatattac attctctcgt attgtgtttg cgttgctaat ggttagttct
     9541 acattctatt cactgccttt ctggatgtgt tatttttggt gtggaattag tgatatactt
     9601 gatggtttta ttgcccgaaa attgagacaa cagagtgtaa taggtgcaaa gttagatagt
     9661 attgccgatt tcatttttgc ggttacaatt ttggttaccg tcgcaaaaaa tattgctctt
     9721 cccatgtatc tatggtggtg tattgttgca atagcattgc tacggttgat tagttacagt
     9781 attggttttt ttaagtatcg tacattttca tctcttcata ctttgatgaa taaaatcact
     9841 ggggcttcta tctttgcttt tccattactc tatatttgct ttgggcttga tattgcagga
     9901 attatgatat gtatcatggc ttttctttct tcttttgaag aaatagcaat tatgataaaa
     9961 tcaaaagaat taaatcgtga tataaaaagt atatttatgt gacaacttag aatttgtgac
    10021 aaaaataatt tatataataa tgaggaagta aacataaaaa attatccaca agtttatgtg
    10081 taaaatagtt ttaaagcaat agagttgtat tgtaaaacct ttagtgcata aattacattt
    10141 taaattaaaa atgatactga aactgcttat cagtactgcg aattatctgt aaatagtgaa
    10201 gactttttag atttaaacta aaagttggga aaaaattgag ttttaaaact tacactcttc
    10261 ttatatagaa aaggagagtg ttttttatga ttataaaagc aaaacaatta tcaaaaatat
    10321 acggttctaa taacaataaa gttatcgcac ttaataatgt aaatttggaa ataaactcag
    10381 gtgaatttgt ttctataatt ggaccttctg gaagtggaaa aagcacactt ttacatattt
    10441 taagtggatt agataatcct acctctggac aagtgttact tgatgataaa gatatatata
    10501 aacacagtga aaaagaacta tctgcactta gaagaaaatc ctttggattc gtttttcaac
    10561 agtttaatct tttacctgtt ctgacagcat ctgaaaatat ttcaatgcct gtacttctag
    10621 ataaaaaaca acctaataaa gaatatttaa atgaaatctc atcattactt ggaattgcag
    10681 atagactcaa tcatcttccc catgaacttt caggtggtca gcaacaaaga gttgcaattg
    10741 ctagagcact tattgcaaaa ccagatataa tatttgctga tgagcctaca ggaaacttag
    10801 acagtaaaag tggcagtgaa gttatgaatc ttcttataaa aacttcaaaa cagtttggaa
    10861 agactttagt ggtaattact catgatgata gaattgcaaa acttgctgat agaaaaattt
    10921 caattattga tggtgttctt atggaggtga aatagaaatg aagaattacc tttcactttc
    10981 aataaaagaa ttaaaaagcc agaaacttat gactacattc atcattatag caatagtact
    11041 atcaactatt atgacaactg tggttggaca gtcaattggt atcctacaaa atttaagaat
    11101 tgaacaagcc cgttctttta acggtgatag acatgtatcc tttcaccaac ttactaaaaa
    11161 tcaagttgat gacataaaga aagatgatag agtttatcaa gctggtactt caataactct
    11221 tggcagtagt aaaataaagg attctggtat ctctgtactt gtaaaagagt atgataaaac
    11281 aggactttcc aactacccca aattaatgaa attaaaatct ggaaatttgc ctaaagataa
    11341 aaacgaaata gccttagatg aaaacacact aaagcttatg ggtataaaac caagactagg
    11401 agtgactata cctatgaatt tagatatttc acttttaaat gatacaatac caccatataa
    11461 ttacacagct aactttaaat tgagtggtat tttagaggat gattatacag gatacgtaag
    11521 tggtatagta aacggtattg taggcaaagg tgcatcagaa aatctattac ccaagagata
    11581 tattttatcc tctttagatt ttaaaataaa acagcaagag aagtttcaag aaatagtaaa
    11641 tcaattagct aaaaaaataa atctttctca caattcaatt caatataatt ggattttctt
    11701 aaatgctctt aaaatacagt ttgaaaaaga tgaaaatagt tctaatagtg atggtatatc
    11761 tatgattata ttggtatctt tatttgttgc cttattggtt ttactggctt ctggacttgt
    11821 aatctacaat attttaaaaa tctctgttac aaagaaaatt aaagagtatg gatgtttaag
    11881 agcaatagga gctgaaccaa accagatata taagatagtt attttacaaa tcttaatttt
    11941 atgtactgtt gccataccta ttggagctgt aattggaatt atatcttcta aaggcataac
    12001 tggaatggtc actaatatct taaatccaga tatacttcta gcaaatgata ataaagaaat
    12061 tactgagctt atacataaaa acacaacagc atatatgttc ccattagtat taagcactag
    12121 tatatctttg attttttcac ttatttcagc attaccatca gcaatatatg cttcacatgt
    12181 ctctcccaaa attgctatgg ctggcagtac taccaaaatc aaaagaaaaa ttaaacgaga
    12241 aaaaacaata aaaaactttg aaagacatct agcctggcta aacttaaaaa gaaataaagg
    12301 aagaactata ataaccatac tatcattatt tatgagtata acagtattcg ttgcactgag
    12361 tgagttctct aatgtattag atgtatctag gtcagtaagt aatttaaaag aaggagattt
    12421 ttccttaaca aatgaaatat ctggttttga taagccaata ctagataaaa tagataacat
    12481 gaaaaacgtc aatcgcactt cttttataaa gtactctgaa tacaaacagg gcgaaataga
    12541 tacagatata aattttgaaa atagtggtga gatgttaaaa atcattggaa ttgatgaaca
    12601 aactttaaaa gacttaatgc cttctattac agattctaca ttagaagact ttaaaaatgg
    12661 ttctatctgt tttataaaaa atccaattgc tatttctaca ccaggtgtaa aaactaaata
    12721 tacaaattta aaacctaaag ataatatcac aattaacaaa aaacaactag atatatatag
    12781 tacggtagat aaaatgttct ttttacaagg taatggctgg gtaaatggtg ttgatgttat
    12841 tgtatatgat tctgtttata acaccttgac aaataaaaac aagttcaacc agataaatat
    12901 ttatgctaaa gataaatcag atttagaaca aataagattg tctattgaac agatttgtga
    12961 aaataatcct ggctctcatt ggatatcata tattgaatca gataagcaac ttaaagagag
    13021 ttttaaacaa atagaatttc tagcatgggc agttatatta tttgtgggat tgattggtac
    13081 tttaaacatc attaacacaa ctcatacaaa tataaatact aggactaatg agattggagt
    13141 taaaagagct attggtatga gtaatagtag cttatataag atgtttttat gggaaggtgt
    13201 ttattatgga atttttgctg ctatatttgg ttcaattgct gggtatgtca gtgctattat
    13261 tataaacatg gcaactatag aaaagcttga ttttactaat ataccaatta cttcaatttt
    13321 acaagctact attatttcta tacttgcatg tattatagct actcttattc cattaagaaa
    13381 agttaaaaaa atgaatataa ttgactgtat agatatcaat ttataactat tttacagata
    13441 ataatataaa ttaaattatc aatgactgta cccattttct ttcaaatcta actaataaat
    13501 ctttttaact ataatataag ctttaaaaaa ggcctgtctc aaaatagaga taagcctttt
    13561 ttcattgtcg aaataatgaa aatatattta acttcacaaa aaaagagtgc ctcacgaact
    13621 aaaaatttcg ttttgagaca ctccccttta gtttaaaata tatagttaaa tgctatgcca
    13681 aaatttcaca catgctccac ctgattttga attttctatt ttaacagaac ctccaagctg
    13741 ttccactaat tgttttacaa tataaagccc aagaccagaa tgtcctcctt tagtctgcct
    13801 agcttcatct cctcgataaa acttatcaag agcctttttt aaatctttag agctaaaccc
    13861 actaccagaa tcacaaatct cataagaaat acgatttttt tcatccttta cagtaatact
    13921 aatattacca cctgatggag tgtattgcag actgttggat atgatattat caaaaattcg
    13981 tgtcaaccta tccacgtcga tttgaatcgg ggattgtata ttaccctgca ttttcaatat
    14041 aagttcaact tccttttgat gagcctgtaa ttcatagtct tgcacctttt gcctcaaaaa
    14101 ctcaggtaaa tttataggaa caagatttaa ttgtatattt gatttttcta agtcagacgt
    14161 atattgcatc ttttggacaa gagcagcact tttttctgta ttttctctta tcactgtaag
    14221 atacctatga agtttttcat tgtcatctgt attattacca ataagtgcat ctgtatatcc
    14281 caaaataata gaaagaggag attttaaatc atgtgctaga gcttccacca tttcaacacg
    14341 ttcctgctcc atcttccact gtgcagataa agagcctttt aattcgtctt tcatttcaga
    14401 aaaggcacta caaagtttac cgagttcatt atctgaataa tagtcgattt caaaatcaag
    14461 gtccttttct tttatcttat tggacgcatc aatcaataga tgtaatggtt gattgatatt
    14521 ctttacaaag cgcttagaaa gccatctagt aaaacccacg atatatagaa atggagaaaa
    14581 cagagccatt ataataactg caaatacaaa tctccctcta ctatttgcaa aggttatttt
    14641 gacttgatag aatagaataa cagctccctc aatctttcca ttatcccctt tgattggaac
    14701 agtatagata taaccacctt tacgaacagt tttattgata aaattattaa ataattcttc
    14761 ttttgtttta aatgtttttt tgggattagt accatataga atattgccat tattatccac
    14821 aacttgatat agcatatcat ccccacgaat cgttcttttt aaaccttctt cattcgattg
    14881 tgataacaaa gctatgtttt tttcattgat gtatttttca attctaggaa cttgtcgctc
    14941 ataatagttt ggtggataga tatccttatt taaggaataa atatacaaat aaatagcaaa
    15001 tacataagta atcactgtgg caactatact ggctattata ataaaaataa aagtacgtct
    15061 aaattgggca ataagggatt gatttttcat ttaataacct ctctctcttt acattttatt
    15121 ccacttatac ccaatacccc aaactgtgga aatgtattct ttttctggtg tcacagcaga
    15181 aaacttagct cgtatttttt ttactcgttc aacaacagtt gaggaatcac cttcagaatc
    15241 acacccccat actttttcat agatttgttc tcttgagaaa acttgtcctg catggagtgc
    15301 tagtaattca acaatatcat actctatttt tgttaaatca atattcttac cattaatttt
    15361 aattgcacgc tccttcatat ataagcataa cttcccaaaa tacaaagtag acctcttgtt
    15421 ctcttcattg atatactgtg aacgcttttc cctacgcaga ttagcttcaa tccttgccat
    15481 aagttcacgc agtccaaatg gctttgttac ataatcatcc cctccaaagg tcaacccttt
    15541 gattttgtcg gtttctgatt gctttgcact taaaaatata attgggcata aaacatcatc
    15601 tctaattgcc tgacatacct caaatccatt cattttaggc atcataatat ccagtataat
    15661 aaggtcaggc tcctgttttg ataactctat gcctgcctga ccatcataag ctatcaacac
    15721 ttcatgtcct tttgccttca attcgtcctc aagtagcatt actaggtcca tttcatcatc
    15781 aataatcaat attttactca atataacacc acctttctct tatgggaagg tcagtattaa
    15841 aacaatattt gtctactcat aacttttcct tccctcccac attttaaacc aaataacgcc
    15901 tcctatcaag aatataatca aacctattgc tactactata agctctatac tagctgtttg
    15961 catcacttta gagtaaaata cctcagttga aatagttcca gcttctttaa taaaataaat
    16021 ccctggaagc atacctagtt ttactggcca tgtccatgga acaaatgccc atactttttc
    16081 accaacgcta gttactccaa gtattgctgc catcaaaatt ccaccaatac taataccaat
    16141 agatgcaccc ataccccatg caaaactcac ccaaagatga atagctaata gtggaagtgt
    16201 cccaatcacc acaagtcctg ctgctgacag gaataaccat attgttgctc cagatggcac
    16261 tgcaatactc attcctatgc aaagtatcaa tgtagcaata aatgtgcata tagttaagca
    16321 aaacagaaga aaaagaaatt ttcccagata taatctaaca cgagaaatac ctacacataa
    16381 aaaaccatta aaatttccag caagctcttc ctcctgcact ataaatcctg ctaaaatacc
    16441 tactccaata ggaatgatga atacagtcca tactgtaaaa aatccttcaa aagcaaattc
    16501 ttgtgtactt ccagatttag ttgtgagata aaaaaccaca cataatgaaa aaataatagg
    16561 catacaaaga gtaagccact ggacagctgt cctctttgct cgaagccatt ctgatgataa
    16621 tagttttatc atttgtcatc cctcctattg aaccaagctg cagtaagtgc tgttaatact
    16681 ataaatacaa ccaaagaaac aacaatccca ataggaatca ctgaagtatt caaaagtggg
    16741 tctccagctt ctaatacagt accgttagga tgtatgttga tgattgggca taccattcta
    16801 actgcccaac tccaaggtac tgcaatccaa aatggttttg tcgcagcaag gacaccaaat
    16861 atcatgccta aagctccaac tcccatactt aggaacatac ctttccatgt agctagccat
    16921 agctggagtg gaagtatagc aagagaaaca acccagcata caatactcgc ccctataatc
    16981 tgtccaaaag gaactggacc agctttcaat attaaaccag ttataattgt tacgaatatt
    17041 aaaactagcg ttgaaataaa actgtagact gccatagcta taactttatt aatccaaagt
    17101 gtcataggag acacattgtg tgtacgtaaa gcacgataat ttccagcttt tttttcctgg
    17161 aaagctacca aagtagcaaa caatgcaaat ccaataggca gaaataaaaa tgaccaccag
    17221 ttaaaaatta atgctaaaag attttgccac gcccaagaag ttgtttgaga taatggattt
    17281 tgcatgagtg ttgattgcat tataaatgca tatgctgcaa aagacactgg gaaaaaaaca
    17341 ataagtttac ccataaatgt tcttttatac tttaaaagtt ctgattgtaa agcattcatc
    17401 tgttttgtcc tcctttaaga ataacatctg taaaaaattc ttctagattt tcattagagt
    17461 caggcattcc ttgaaatagt agtcgtcctc catttataat tcctatatta tctacaactt
    17521 gagcaacctc tgatagaata tggctagaaa gaattacagt tatacctttt tttggaaaag
    17581 aagcaatcaa ttcacgcaat tcttgaatac caaaagggtc aagaccatta gttggttcat
    17641 ctagtattaa caactctggg tcactcaaaa gtgcaatagc tattcctaac ctttgtttca
    17701 tacccataga aaattgtgaa gctctctttt tacctgtatt tttcaaattt actatttcca
    17761 acacttcata aattttatct ttaggtattc ccaatatttt tgtatgtact aacaaatttt
    17821 cctctgctgt aaggtttcca tacaatgcag gtgattctat taacgaacct atttttgcaa
    17881 gagattctcg tttccaaggc tttctatcaa aaataatacg tccatctgtt gggcgaagaa
    17941 gaccaacaag cattttaagt gttgtggact ttcctgctcc atttggtcct aaaagtccat
    18001 atatactatt tcgttcaatt tgtagagaaa cattgttaac tgccatttgt tctccatatc
    18061 tttttgataa gttttttgtt tcaagaatta aatctttcat attaatttct cccttcttgt
    18121 aataccttaa atctattact aagagaatac taacatgaaa aaataacaat tctataacac
    18181 ctactaattt taataaaaat taaaataaaa aatagagtta ccaattttat tttggcaact
    18241 ctatttaagt ttatgtttaa cagttatatt agatttaagc ttagtttcat tatcacacta
    18301 acttcaaatt tactctataa ttcttaaatc aaacactaat ctattaattt atatttcttt
    18361 agacatctaa tatagtttgc tactaaataa taattattta tatcatattt atctaatcct
    18421 ttaaaagcag ttatttcatc attaagctca tcactaccat ctacaaaaac acaacctgtt
    18481 tcaaattcca tacctacaat aatcttacag ccactataag tttcatcttg agtatcgtac
    18541 aattttattg catttccttc attttgaatt gtaaatacaa tagattttaa ttctttatca
    18601 ctgggaatct tttgtgaaaa aggcttgttt aacttaatat tattgataac tacatgttta
    18661 aattcctctg aagtttcttc tactattaca tattttcgtt ttaaaaactc tattatttct
    18721 aaaacagact tagaatttac ttttaaaata cttttatatt tttcatatac agatttccac
    18781 tgttcaattt tttcctgtgg tacttcttgg atatgtgtac ttattttttc ttgagatatt
    18841 tttttcaatt aaactctcct ttcaatactt aaatattctt atgaatctca catgactaaa
    18901 gttataagat tcctacttaa tagaattttg catactaaaa tatgtcttac taattctcta
    18961 caggatatct ccgtagttcc tacgattctt acataagtta tctattttaa attgttatta
    19021 gttagattct aaaaattatt tgttttatca ctaatataat actcttaaaa actttacaaa
    19081 tcaatttaaa tttgctacct tttatttata acaacttata gtacatttct cctatgcttt
    19141 tcaatatatt acttttaaaa ggaggttttc cttatgattt taaatagaac tgagcttttc
    19201 cgaggatatt ctttaaatag atttaacgag atatgcaata tattatcagt aaataaaatt
    19261 aaatataagt ataaagtgaa aaataatgct tactctaaaa aacctttttt taatgaaata
    19321 acacttggca atttaggtca aaaggaagat ttctcttatg agtacttaat ttatgttcat
    19381 aaaggcgatt atgagcaggc agaagctcta ataaattcta agttgcgata gattatttat
    19441 aaaacatgac attctgttgt catgttttat atgttattat actttcaaat atactactat
    19501 attttcaaaa ttgtgtgctt taaaactacc tttttatagt aatttaaaac ttaaaactaa
    19561 ggagacttgt tatatgaaaa taaatataaa atcaacatta aatgaattta ttaaacagaa
    19621 taatcttttt gatgacaaca gaataatatc ctaccttcct aatataacaa tagacacaga
    19681 caaaataaaa actataatga ttaatgaagt tgtcccaact aaccccaatg acgattttta
    19741 tagtatagat aaagatgctg attatttaaa aactactatt cctttattta acaatgcagg
    19801 aatagtagta tctaacataa atgatatcct agatatgggt atctatataa ctaatgccgt
    19861 aaaacttcct aaatcagaat atacaataac tcgtgataca attaaattac atatgcctat
    19921 tttagaagaa gaaattaaat tgtttaaaaa cctagaggta gtaatgttaa tgggagatgt
    19981 tgcaaagaaa tcttttaata tgattactaa aaaacatata aaaaagaatg tcatcccttc
    20041 catttcaaca tataaacttc gtaataatga actttattat gataatttaa gagtatttcc
    20101 atcatatatt atgactggtg gaaacatact aattgaaaaa tcaaaatttg aaatggcatc
    20161 agaggatata aaaaaaatgt tcgagattat aaacggttga tattgcaaaa taataaaaac
    20221 taatttagac aaataataag tgcctcaaaa acttctattt atgaggcact tttattttat
    20281 attagtgtaa ctatatacta ttaacataat ttgttattta tattgttctt tttaatctct
    20341 attccattta gtctaggtaa cattactctt tacaagatta ctttatattt ctcttatatc
    20401 tctaacattt ttatttaata aaacaattat taatacaagt caattatatc tatttatcta
    20461 aaaatatatc tttcacagta atatctggat ggtgatattt cttcctatcc gttaaaaaag
    20521 atttttttac attaatagcc tcttttggac accagtgaaa acaagctagg catctttcac
    20581 actcattacc ccactttggc tttttatcaa ctatacttat attctctaca ggacaaaccc
    20641 ttttacatgt cccacatcca ttacatttat tttctgcaaa aaatttagta gttgcaattt
    20701 gcatattctt acctactaaa cgaagtacaa cactatctat atgtgacttt aaattgtttt
    20761 ttccttctat atttccaatt tccaagttat tgatacattt tgcaatctca gctaccctat
    20821 tagtctgatt tctcaatctt tcttcttcaa catctggagg tgttatcaaa gcatttccag
    20881 gcatattaat taaaaaacca gcagataatt ttttctcctt ttttttaagt aacttatcta
    20941 tagtaaataa actatgtcca gctaccccat tacaagtggc aatggcatat atatgagtat
    21001 taatattaaa atttaatttc tctacaaaag actttactat atcaggaaca tcagcaaaat
    21061 aaacaggaaa aacaattacc aatatatctt catttatgtc tatattattt tcttttctat
    21121 gtatagctaa aggtataact tctttacttt catttaacat ttctttcaaa gtctttgaaa
    21181 ctactagcga atttcctgta ccagaaaaat aataaatctt actattcatt cttatccctc
    21241 tttctatctt tctgcatcat caatagttaa aaagtctctt aaaataggat ttatatttac
    21301 tttccaattc tatccaaaat atcagtaaac aaaggcaatt cttcatcaga gatagattta
    21361 aatgtctctg ttgcatatac atttgcaatt ttatttaatg gttcttctaa ttctctacct
    21421 aaatctgtta ggaatatttg aaacttacgt ctatctttaa catctacttt acggacaatt
    21481 aatcccttat ttttcataac atctaagatt cttgtcgtat tagatttatc cttacatgat
    21541 ttttcacata aatctatctg agaaattcca tcttgctccc atagaaacct taataacgca
    21601 taatggtcag ttgttatatc atatccattt tgtttaaaca tcttttttaa atcatctttt
    21661 actttcaatg aagtacttac taacttagtt gtagcttcat atagtttctc aaaatctttc
    21721 attttacctc catttaaata atcccttgcg cctcccatct atcaattatt cccttatatt
    21781 gctcaatgtc tttaaaagcc attggcttta aaacagcatt tatagcaata aaattatata
    21841 tttgagaaaa cacaggtgga tttttctttt cctctatgca ataaataaat tttttggata
    21901 ccttttgtat ctttgattct actttttcag ttataggatt ttgattaaat ccagttttta
    21961 tagataaact accacaaaca taacctcccg aatctaagac cattgatttt ataaaagaaa
    22021 ttgcttttga accacgagca ttttcatatg tagtaactgc tatacatggc ttacgataca
    22081 ataatctttc aagcatcata tgtgctctat ctgtaaacac tttgaaaagt ccagttacac
    22141 tacttccata agttggacta ccaaatataa caccatcact cttagtaatt atatcatgaa
    22201 tatactctac tttatcattt tgattaatac acttccccat tttataacaa tttaaacaac
    22261 caatacaata ggatggattg acctcactta aatcaaaata attaattttt acatctggta
    22321 ttaatctttc tatatcttta tacaaataag tcaaaacttt tgatgtagct ccattttttc
    22381 tagggcttcc atttataact gtaattatca tttctatctc tcctttttta gttgttatat
    22441 caacaataag tatatcaact atatttttag ctgtcaatat aaacccttta tttaaagcta
    22501 tacttacaca gcttaaataa aagttttatt taactacatg tacttattta atcaactata
    22561 aatgaattat ttttaataat tgacaaacta aattaatgat aatatacttt atgtattaca
    22621 aatgtaagta gaaggaggta tagcatgaaa aagttaccac atatttcaga agcagaatac
    22681 caagttatga aaattatttg gaaatatgct cctatcagca ctactgaagt aattgaaaag
    22741 ttggtagaaa caagtacatg gagtcctaaa acaattcaaa caatgttatt gagattagtt
    22801 aaaaaaggag ctttaacata tgaaaaaaat agcagagtat tcgtttatac tccacttgtt
    22861 aaagaagaag aatacatagc tacagaaagt agttcttttt taaatcgttt ttataatggt
    22921 actcttaatt ctatggtact aaacttttta gaaaatgata aactatctga agatgatatt
    22981 gaggaactga gagaaatctt aaataaaagg actactaagg gggataaata aatatgtatg
    23041 ccccattctt tatacgattt ttactcagta cattaacctt atcaatatta attgttgtaa
    23101 ttttacttgc caaaaaagta tttaaaaaac atatttctgc aaagtaccaa tacaatattg
    23161 gatttatatt gcttataatg ttaatacttc catttgtccc tcttaaatat attaatcttg
    23221 gaaatgtatt taactattta tatactttta atcaacttgg aagcaatact aataggactt
    23281 ctatgataaa aaatagtcca aattctataa ttaataactc aaatgcatta aacgattttt
    23341 cagtatccct aaaccaagtt aattttgata gtttaaatat tttattacta atatcatgga
    23401 taattggaat tttaatcatg atagctatta ctattcattg caacattaaa ataaaaaata
    23461 tgaaaaaatc aatacaaatt ttaagagatg aggagatagt ttttacattt gaaaagtgta
    23521 aaaaagatat tggtataaat aaggatttta tactatgcaa atcacctatt ataaatacac
    23581 ctattacatt tgggtacttt aaaccacaca ttatactacc agataagtct atatctaaat
    23641 tatctttaaa agatataaaa tatatcttac ttcatgaatt acagcatttt aaaaatagag
    23701 atatactgat aaattatata atgtgctttt ttcaaatatt atattggttt aatcctcttg
    23761 tatggtatgc atttaaagaa atgcgtatag accgtgaaat tgcatgtgat atttcagttt
    23821 taaaaaagct tgataaagat agttatatag aatatggaca aactctaata aactttgtgg
    23881 ataatatttc aagaccatca aatcttacac ttacatcaga tattggtggg tcaaaaaaac
    23941 aaataaaaaa acgattattg aagataatgg attttcatat tgaatcaagt gctttaaaat
    24001 tcaaaagcat tttaattttt acattggtag ctttaattgt atttggaaat attcctagac
    24061 tatctattat ggcagatatc aatgaaaaat atagacctac aaatgacaaa acggtgtacg
    24121 aggacttaag tcaatatttt aaaggttttg atggaagttt tgttatgtat gatttaaaat
    24181 ccaaacaata tcatatttac aataaagaaa aaagtaaaac aagaatttct ccaaattcta
    24241 cttataaaat ttatagtgcc ttatttggat tggaaaatgg gataataaca agagataatt
    24301 cacatatccc ttgggatgga aaacatcatt ctaatagttc ttggaatgaa gaccaagact
    24361 tattttctgc tatggaaaat tccgtaaact ggtactttca gactctagat aaaaaaacaa
    24421 agctaaaaaa catacaaact tatcttaagc aaattggata tggcaatcat gatgtttcag
    24481 gtggtatctc aaacttttgg ctagaatctt cattaaaaat atcacctatt gaacaggtaa
    24541 aattacttga aagtttttat acaaataagt ttaaatttga agataagaat atagaagctg
    24601 taaaagattc attgttaata tcaaaaaagg atggtgtatc actttttggt aaaacaggca
    24661 ctgccaatat aaatggaaaa gatgtaaatg gttggtttat aggttatgtg gaaaaaaatg
    24721 aaaatactta tttctttgct acaaatattc aaagcaaaga ttattctaat ggtagttcag
    24781 catctaagat aacattatcc atattaaatg acaaaaaaat ctataatcct taatatattt
    24841 actgtacatt ctaaaaaatt cacataattt tcatattaat tatcgattaa attcatatac
    24901 aaggcttatt atataaacat agaagatagt aaaactagct taaaaacata atataatacc
    24961 ctatacaaat agtggcaact ctccctcaca tcctcaattg ttgccactat ttaatttttt
    25021 gtatgaagtt aaatatattt tcatcatttc aacaataaaa aagaggtcta tctctatttt
    25081 gaaacaacct ctttaattat tttagagttt actaagaagt ctaaaaacaa tgtatctaaa
    25141 atattattct caactgagta agattaattt ccaaattgag taaatttaat cttcaaatac
    25201 tgatttaaca ataggaataa cataatgact tcccccacga ctctttacat cttcaatcat
    25261 agcaattatt agatattgtt tatcactatt ttcatctaca gtaaaagcat taaaccatcc
    25321 aagttctgtc cctgtaacat catcttttga tgctttaatt tctgctgtac cagttttccc
    25381 agcaatgcta attcctttaa tcatagcact atgacctgta ccatcagggc tttcaacaac
    25441 ttgaagtaag tcatcacgaa taatttttac agcctcttta gaaaacactc ctttcttcca
    25501 aaattcagcc tttttaaatt caagatgggg ttttatcatg tctccatcat ttacaaaagc
    25561 tgaatatatt gatgccgtat gtactggatt tacaagaagc ttaccttgtc catagccagt
    25621 atctgctaat tgtatttcag tctcaaattt attgtcagtg ccaaattttg aagaagacaa
    25681 tccaaattca aatggcatac tttcatcaaa tccaagcttc aacaactctt ttgctaatac
    25741 atcttcacct atttttaagg ctgccttagc aaaataaata ttgtcagaat aaatcaaagc
    25801 atttttcata tttgcagtat ttccataatc ttttaacgtg gttaccttat aacttcccca
    25861 actagaatct ttttgccaac ttagactgct gtgtccaaag ttttcatttg gattaatctt
    25921 tcctgttgtt ataccaatac cagcagtaac tggtttaaaa gaagacccag gacataatcg
    25981 tgctttaaat ctattgtaca ttggtttatt ttcattttta ttgagttcat tccatctatc
    26041 ttctgacatt cccaatataa actcatttgg attgtacgac ggactactta caagtgataa
    26101 aacctctcct gttttaggat tcataactac tgcacaacct ttatcatttt tcatttggtc
    26161 atatagttta ctctgtagtt ttgaatctat agttagcttt acatcttctc catctacctt
    26221 tggctcagtt actaaagttt ctttcttgtc accatatcta tcagctatta taatctcata
    26281 tccatcaata ccacgtattc tatcttccag taaattttct aaaccaatct taccaattat
    26341 actattatcg ttgtacctct taccctcttt ttcctttagt atctctgcat ttacattttg
    26401 aacataacca gttaagtgac cagttttttc accaaatgga tatactctaa cctctgtatt
    26461 agatatttta actccaggaa tactaagaag tgactcttgt agttccttat cttcttcttt
    26521 cacagtacca tctgcatttt cttttatttt ttcaatagtt tttataggta ccattgaatt
    26581 ttcttttacc caagatgcat ttaatttctt ttcaatttgt tcaactgata cattcaataa
    26641 agtagataat ttttttaagt cttctatatt atcactcatt tttccaggaa ctaatcctat
    26701 catagatgca agaccttgac cagcaatcat tacaccgttc ctatccagaa tacgtcctct
    26761 cttacctttc agctttgata cccttacagt attatcttct tcaagttctg gaaatatgac
    26821 gtttgactgc cattttattt tataattttt ctgtctatct ctagttaata gtacttcatt
    26881 tgaaaatgaa atttcacctg ctaaagtatc catcttagaa tcatagttta ttactttatc
    26941 attattttta ttttttttaa tatcattaat cttaatactt atatcgtgtg catcaattcc
    27001 ttcatagatt tttttatttc ttaaaacaaa atcttctttt ttattttcag acttgctctt
    27061 ttcatctaat aaatcataca ttttctcata ttgtttttta tttgcaaaag acatatactg
    27121 cttaaacact tcatctggtt tgtttttatt gagtccatct agtatgaatt ttgctaaaaa
    27181 aacaataata actataagca aacacattag aaaaccaaaa attatcattt tttttgtttt
    27241 tttatttttt ctcatataaa ccacctttat catttaatat attacatacg taagctttaa
    27301 ggttaaaata ctcttataga catagctaat ctatgttttt attatgtttt taagtatctc
    27361 ataaaaatat gtaagcagta taattgatag taatttatat attacacatg taagttttaa
    27421 aagtcaataa tattatcatt atttaattat tttttacatc aacttaaaaa taaatcagtt
    27481 ccattatatt cttttgtatt cttcaactat tttataaatt aaccttattc tattgtaatt
    27541 attttatggt atataacttt aacttgacaa aacgacacaa gtgtctcata attaaaagtg
    27601 acacttatgt cgttaaaaag gaggttaaaa catatgccaa caaaaggaga acaaactaaa
    27661 caatttatca ttgatacatc aataatgcta ttttctgaaa aaggatatag taatgtgaca
    27721 atgaaagata tatgtgaagc atgtaattta agccgtggtg gcctatatag acactttaat
    27781 tcaccaaaag aaatttttat tagtatatta aacactcata aatactactc tcaaaatttg
    27841 ttggataaag gtatcaagga aaatatatca gctaagcata tgctagaata ttttttccaa
    27901 caacaaaagg ctgacataaa tggcaaatat tgtggatttg attttgcaat acacgaattt
    27961 gcttttgtag aaaaagacca acgtgattat atgaataaaa gatacaattc agctatcaca
    28021 atgctttcaa atttaatcga ttatggacaa tctaaaagtg aatttaaaaa atgtgattct
    28081 aaatcagttg caatacacat aatgttctta tttgatagca tgaaaacatc tgcttccatt
    28141 ttaaaactta caaataaaga cattgataat caaataaata ttataaagga catggtgatt
    28201 ttatatgaat aaacaaaaat tccataaaat tgatttacaa aattgggaac gatatacaca
    28261 tttttactac ttttcaaaaa tgacaaatgt agggtttaat ttgactatag aaatagatat
    28321 tactaatttg cttaaggaaa cgaaaaaaag aaatattaaa ttttatccag cgtatttata
    28381 tacaataact acttgcttaa atcgatatag agaactaaaa atcgcatatg atgaagatga
    28441 actcggatat tggaacacgt taacgccact ttatgcgatt tttcatgatg ataataagac
    28501 tttctcatca atatggacag aatatgatga aaatttctca atattctatg aaaattattt
    28561 agctgatgtt aaagaatatg gaaaaaatca tggttttcta gcaaaaccaa atccgccaag
    28621 aaactgctat actgtttcct gccttccatg gattagcttt aagagtttta ctgtacataa
    28681 tcatggtctt gaaaattatt ttttccctac aattgaagca ggtaaatact atcaaaaaga
    28741 tgaaaaaata cttcttcctc tttctataac agtgcatcac gcaactactg atggatacca
    28801 tgtaagttgt tttattgatt acatacaaaa aatgtgcttt aatactaaga tatggctaca
    28861 ctaaaaattt atacaatata aaaagaactg tcgcatgagc aaattaaatt tgctatgaga
    28921 aagctctttt ttacttacca atagttaata tgagatgaat tatttcttat accttgaact
    28981 taccaatagt taatatgaaa tgaattattt ctcatacttt gaaaatattg aattgatttt
    29041 gataatcttc ttttataatc attacgtgtc acctactact caacacatat tctttgacct
    29101 atttttacat ccttgtttat atccaaaata tttaccttct taccatctgg aaacaatacc
    29161 attgtcaaag tagactttcc taattcgtgt attctcttta aatcaacatt aacaagaaca
    29221 tccccctgtt taaccatatc tccttgttta acagcaacat caaagccatc tccatttaat
    29281 tcaactgtat caattccaac atgaattaat acttctccat tagttccttt aatcccaaat
    29341 gcatgtccac ttgaaaatac tgtttctaat ttacctgaaa taggggaaac aatttttcca
    29401 tctataatat caatagcaaa accatcacct aacatttttt tagaaaaagt ttcatcaggt
    29461 acattttcta atgcaacaat cttaccttta gctggagaca caaagttttc attattttca
    29521 ttaaacacta attcttcgtc ttcatcttca tttatagctt ctttaggtac tccaaataaa
    29581 catgttgcaa taaagcagaa tacaactaca aatattgata ctaataagta tgtgattaaa
    29641 ttatatccat catagatata cattaaataa gaaggtaaaa ctgctaatcc atatgaatta
    29701 gattgaatat gtaaaattga taatattgct gctcctaaac ctgaagatgc acacataata
    29761 atcaaaggct ttaaattata tcgaagctga atcccgaata aaacaggttc acttatacca
    29821 aaaagttgag atagcattgc tccaactgca gtttgtttaa cttgattatt tttagatttt
    29881 ttcataaatg ctagacatgt tccaatattc gcaaatccat acattgcaca aagagtaata
    29941 agtgcattaa atcctgtatt tgctaatagt gatgtttcaa tcattacata agtatgatgt
    30001 aagccagtaa taacagctaa tggatatgta aatccaataa taaatcctcc aattcctaaa
    30061 ggtaaaccaa tcaataagct gatgatatca accattttta attctattgt atgcattata
    30121 ggccctacac ctaacaaaat aactaacatt gttattagca taacaaagaa tggtgtgaaa
    30181 ataaggtcta atacattagg catatgttta cggaaccatt tttcaagatt agctccaata
    30241 aatgctgtaa caatagctgt taaaacactt ccttgacagc ctacaatagg aataaatcca
    30301 aatgccataa ttgcttcaac ttcatttgat gggtcagcaa cagaatatgc attaggaagt
    30361 attggggaaa ctaacataag tccaataaca agtccaataa caggtgttcc tccaaatacc
    30421 ttaaatgctg accatacaat aattgctggt aaaaaagcaa atgctgtttc tgtaagaacc
    30481 gaaacaagtg tctgaatata caatggaata tttgcagaag acattccaaa taatccaagt
    30541 acattatcat taaacagaca accctttaat cccaagaata atcctgttgc cgcaataact
    30601 ggaataatag gaacaaatat atctgctaga gttctcataa ctttcttaaa tccagtttca
    30661 ttatttttaa ctaattcatc ttgttctttt tttgataaag ttttcaatcc cattttttca
    30721 acttctgcat aaactttatt gactattccc gttcctaaaa taatttgata ctgtccagaa
    30781 gtaaagaaaa cacctttaac ctcattaacc ttttccactt tcttatcatt aataatttct
    30841 ctatctttaa ccatcaccct taatcttgtg gcacaatgtg taatagaaat aatattattt
    30901 tcaccaataa tttcaataag ctcatttgct attttattat atttattcat ttttctacct
    30961 cactttcaat aatactgctt gaaatggttt caaactcact gttttattta gtagatttat
    31021 atcatctaaa ttttggaata caatatcata tttttcttct gtttttattt ctacaggttt
    31081 atcagaaaaa ttataataac aatcaataac taccaaatct aattgccttc tatacgcaat
    31141 aacattgtca ctttctatat caataggttc tattgagcca taaatcaaac aatcagaata
    31201 ctcactatct tgtctcaact ttatcatctt tttataaaat tctaaaatac tttctgaatt
    31261 gttaatctga ctctgtgcat taatatcttt ataattttca ataaccttta accatggtgt
    31321 tgtatctgaa aaacctgcat tttctttatc tgaccatgga aatggtgttc ttgcattatc
    31381 tcgacttctt aaattcacaa attctaacgc ttcttcttct gagtaacctt catctattga
    31441 acgatagtat tggtcaatgc tagataaatc attaaaatca tttatggtat ttcttttaaa
    31501 atttgtcatt cctaattcct gtccctgata aataaatggt gttcctctta aaaagaaata
    31561 cattgctccc aacattttta ctgcatcatc attcctttcg tattgcaaaa gatactttgt
    31621 tgtagctctt ggttggtcat gattttcaat aaaattagct gcccatccat gttgctgaac
    31681 atacatttga ctatctaaaa tgtattttct taaatctgaa attgtccatg aaattctttt
    31741 aaaccactca ctaccagaag caacatctaa gtcagcatgt ttaaagtcaa ataccattga
    31801 aaaaaaacca tcttctccaa taaattcacc tagctggtca tagctaatac ctgctgcttc
    31861 agcaacagtc atacaatcat attttttaaa cgtattttct tttaattcat aaagcatttt
    31921 ttctattcca ggctgatttc taactgcttt tgtacattta acaagaccat caacaccatc
    31981 actttctaaa ctttgaaaac ttaaattctt tttgatgaat gtaatagcat caactctaaa
    32041 tcctgccact cccatttgag accaggtatt aatcatttgg taaagttcac ttcttaatat
    32101 tggattttcc caattaaggt caggttgttt ttttccaaaa gaatgaaaat aatattcgtc
    32161 tctattctct accttttccc aaacacttcc gccaaaaaca gacctccagt tatttggcaa
    32221 atcgtttcct tttttaaaaa tataataatc atgataagga ctttctgggt tttttaatgc
    32281 ttcttgaaac caaggatgtt catcactcgt atgattaaga actaaatcta atataatctt
    32341 aattcctcta tctttacttt ctttaattaa ctcttttaaa tcttccatac ttccaaattc
    32401 acttgcaata tccaaataat cactaatatc atacccatta tcgtccatag gtgatttaaa
    32461 aattggacat aaccacaatg atgtaatacc taaatctttt aaataatcta aacgagaaac
    32521 aattccctgt aaatctccaa ttccatcatt attactatct tgaaagcttc ttggataaat
    32581 ttgataaacg atttcttttt gccaccattt cttttccata aattactcct ttttatatga
    32641 tatttcttct ttttaattct ttataaattc cattatcgaa tatgtcctca catatagatg
    32701 ttgctatatc tttaagctgt tgatgactat ttttcattgc aattgtaata tctgatgctt
    32761 gaaacatgac gatatcattt tgaccatcac caaaacatat ggtttctttt tgagttatcc
    32821 ctaatctttc ttgtaatctt tgaattgctt tgcccttatg aaaatctttt tgaataattt
    32881 cataatactg actagaaata tccctctgcg ctagttccat tttatcttgt agtatatctt
    32941 tcacctcatc aaacaccttt tcatttgacc ataaacaaat tttatgtata tcttgtgact
    33001 tatactcttc aatgttattt tcatatgtta ttttttcttg gatatgttgt ttatttatac
    33061 aagaattagt tcctttcaac tgattcattg tttcaaatat ttctttggct ttttgattca
    33121 tgaaaacttt ttcccgactc tcaatcgaaa aagcaacttc tctctttttt aataaacaaa
    33181 caactttctt tatcaatctt tgattaaaag attgattata aagtaattca ccatgatatt
    33241 gaatgtaatt acctcctcct gcaatatatc catcaacccc taatgataaa acatcatctt
    33301 gtattgtacc catacttctt ccagtacaaa taacaacact gcaatgattc ttttgacata
    33361 aacgtatagc atgctttgca gattctggga ttccatctac ctcatctcta agcgtaccat
    33421 ctatatcaag tattaataat ttgtacaatt tcatcttcct ttcttactat caaaatcata
    33481 atagcattca aaaaaaaaga tatcaatatt ttgatatctt ttaaaaaata tgttcatttt
    33541 tagcacaaat tttcattttc ggggaatatg ttcactttct aaatacatct tgtatttaat
    33601 ataaagcagt tcaattaaaa taaaataagg tgttgttaca tcgtagtcca gttgtttatc
    33661 tacagaaata gatatagatt gaatatatag gttttcatca cataatcttg ctaaaggatt
    33721 acttatcatt tttgtaatag aaaccatata aattcctttc attttcattt ttttagcaaa
    33781 gtcaataatt gtagatgcat ctccatttaa tgaaataaag aaaatcatat catccgtttt
    33841 tataaaatga ttaagtgcct gaatcatatc atagccataa acatcatata taattttccc
    33901 tgttggtaag aaaatccttt tcatctcttt agcaactctt tcctgagaat aaccttctcc
    33961 aaagacgatt atacgatttg cctttttcat tctttcaaac ataatcgaac aatccttctt
    34021 ctctatatca tctataacct tattataaca atccattaat tggtgtttac tttgattttt
    34081 atattctact tctgtttcat ttaagcgtat aataactctt aattcactat atccaggtaa
    34141 ttgtagtttt tgagcaaaac gagaaagtgc acttgtagat atatggtatt tgtctgcaaa
    34201 ttcatctata gaaagtttaa tacaatcttc tttatgatta agaatacact cacagatata
    34261 cttatcattt gctgtaaatg aattataata tttattgaaa agcccttcaa ttttcattct
    34321 tttctccttc tatactctat gtaattcata tgaagcaaat ctaatagagc ataaaatgct
    34381 cctacgactt cataagatat agcttgtaat gtatcaagtt tttgcgtttc aacatacaga
    34441 ttatactcac aaagtgttgc aattgtatta ttatcaagcc tagttaaaga aatcgtatgt
    34501 atatgattgt ttataaattt tgcaatttca attcctgctt tcgtttctcc ggacaaagaa
    34561 atgataataa acaaatcact tttttgaaat gattctctca taaattcaat ttcacctata
    34621 tcaaataagt caataacaca tttacctact gaaagaaata ttctctttat ttcctgagct
    34681 aaacttttct gttcgttacc tgtcccataa atataaattg tttctgcatc atatattatt
    34741 ttacaaatat cttgaaatga tattttttgt aattcattca ttaatagtcg ataattctcg
    34801 caaacatcat gaaaattatt atcattatta acaaattcta gttgattatt tttaagtaaa
    34861 tattttaaat cagagaatga atctaatcca atttttctac aaagccttaa taaagtagct
    34921 ctagaaacat tacattcatt tgcaattttt tcacacgaaa aattacggta ttctctttga
    34981 caaatcaata tttgtttaat aataatttta tcattttcat ttaaattatc ataattttgg
    35041 ttaactaacg attctaatct cattttttcc actccctaaa tattatttta atatacatta
    35101 gttggttttt ctagggaaga gtcttaaaaa tatttctatt aagacaaata aaaaagagct
    35161 atatcagaat aaatttaatt tgctcatacc acaattctat tataaataaa aaatttttca
    35221 ca
//
LOCUS       DAEPXF010000019        34860 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain HB27
            SAMN13376389-rid12479423.denovo.020, whole genome shotgun sequence.
ACCESSION   DAEPXF010000019 DAEPXF010000000
VERSION     DAEPXF010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13376389
            Sequence Read Archive: SRR10518432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 34860)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34860)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001069624.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001069624.1
            Genome Coverage       :: 315x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/15/2021 01:54:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,165
            CDSs (total)                      :: 4,076
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 89
            rRNAs                             :: 10, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 10 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 19 of 69
            Pseudo Genes (incomplete)         :: 44 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 10 of 69
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34860
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13376389-rid12479423.denovo.020"
                     /strain="HB27"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="China: Hebei"
                     /collection_date="2017"
     gene            complement(147..407)
                     /locus_tag="KRL67_000845"
     CDS             complement(147..407)
                     /locus_tag="KRL67_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH1521750.1"
                     /translation="MDKLITELSSLGAIGILCALLFKNTMQEKKEDRDMYKKTVENFI
                     ELSTQQQEINKNILVQMGIMKTDVEEIKEDVTDIKGMLQNGV"
     gene            complement(670..1023)
                     /locus_tag="KRL67_000846"
     CDS             complement(670..1023)
                     /locus_tag="KRL67_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH1521751.1"
                     /translation="MFKFDKNKIEQIKQGRKVEMQYKDISDISIGQVKQDDDITNNFI
                     ANAEIYEMLLSQSSVNEASNISTFSVRKSGGESGMVEVYVALILRGKKTIEEVPAVIR
                     EQVRIRCKELEIPVE"
     gene            complement(1040..6271)
                     /locus_tag="KRL67_000847"
     CDS             complement(1040..6271)
                     /locus_tag="KRL67_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome condensation regulator"
                     /protein_id="HBH1521752.1"
                     /translation="MKQNKLLQRGAYFNDKNILIDDFDKRYNDYDFVEFFTGISNSTF
                     GLKSDGNLYACGDNTGFQLGLGKDSSERRMFSKVKIDNVKYVSCGSKHSVAVTKDGFA
                     YGAGTSNVGQLGVIESTVYYEFTKLPIDDVKTVACGYDFTFVLKNDGTLYSAGLNSSG
                     QLGLGDTNNRATFTKVNIDSVKDVVTYNQSVFIIKMDGTAHACGLNSNGQLGINSTLN
                     KSVFNKIEGMDNVKQIACGSSHTILIKNDGTMYTTGYNGVGQLGTGNNNNSIVFTLSS
                     INNVKYASCGNNHTMILKYDNTLFSTGQNNYGQLANANKDVASRNTFAKVNVENIKDI
                     KCGSQFNFLINGSKEIFVSGCNLAGQLGSFFHTTFLYEFSKVQSSNLDNYSGLLVNDD
                     YLYVTKDNSEFLNVKLSDNFQDYKKIELTDNNMFIVMNDGSLYACGLNNAGQLGLGDT
                     VNRSVMTKVDIDNVLDIKGNGSSTFVLKNNGTLYSCGLNSSGILGLKDNTNRNIFTKI
                     EIENIKEFCVESNYIVALNHSKELYGWGNQSYIVYGDNRNYPYKDTRVSNVEKIATWS
                     DTLYILDSTGAAKTIGYSYNGSGGYPAPSTSSSYQSKGYNAWNTSYRTLEFYNTAQTK
                     LINLFAFYRGCMFFDESDRAYCIGENNMKFTSSSQITPESELRFSSNSGIYHTNSDGG
                     VYTCYQWTYKLIRCSVFDSSKSVVGNSKNILSLLKNNSTFRCTGSCLTYGQTNQNWSS
                     YLSDNCNGAVSLGNEFILKNYSGESVLKGYGKSNNGEFGSSTSISNASNYDTGLKDIK
                     DIIVKNNTVVVVDKNNNIYVTGTNQFNKLGIGEYNNQPIKKFTNITEQSNSFIFMDDI
                     KEITTSRNTMFIVKNDGTAYATGNNSSGQLGLGDTINRNKFTQINIDNIKKISTSIDG
                     NTTFAIRNDGTLYSTGLNTKGQLGLGDIVNRNTFTKVNIQNVRDVVLGTTHSHAIKDD
                     NTLYSCGENTHGQLGLGSESNHPDVLTFTVNNITNVRDVYCSDTTTFIVKDTNIAYCC
                     GYNNNSQLGMGNTTDQYSFIKCMENVKEVIPNEINTYIITIYNTAYSTGLNTDYCLGL
                     NSNSNQSSFSEIPISNVVKVAPNRNNAVLLLTSEGDVYTAGKCSNGSGTGSETPEKIK
                     KIASKAKDIGMNYRCGHYVSDNGDLYGTGFNNNGQLGVGDVTKRDTFIKTNTRVKKIL
                     PLEYANIAIKDTNDIYICGLNNYGQLGVGNRYDSRNNDNRIFNYKHMNFVMGDLTSIK
                     NRHNFILLNNKIVIPTTKDIDYGLVLGNLYKGDLYTELPYEDIKEVSISKTHIIILLN
                     DGTMYGCGTNYHGELLQDLSINQVDEFVQINVSDVKHVSCGDNFTYFIKSDDSLWSIG
                     KNSEYQLGIGHNNPVTELQRITTISSCKEVHCGKNYTLVVTTGNELFVQGYNDKGALG
                     LGSDSENTIIKFFTKALTDIREIKSYGSDHILVLKNDNSVWVTGKNRDVYKIEQPVEF
                     LKEFTIIPISEDVNTVKDVLATDNTLYIISEVGTTNAAIEITEKSISSIKIKIQDPNK
                     DISRIEMLINGESVKSVSDLITEKISFEVPPDKIKIGENKILFRAYCKGDDLYASLFI
                     FKESTGNSIIKDSYVMIGNRMYKVVNTTSNEQDITITLDRGLEEDLNLGDPIYQLINK
                     TKVQVKINKSDLFKDMKLVEIKKSDSSYQEIYELEEANIKSAQPKIIVEKGDKWTAIK
                     RPSMIFRYDAENNEPQA"
     gene            complement(6293..7279)
                     /locus_tag="KRL67_000848"
     CDS             complement(6293..7279)
                     /locus_tag="KRL67_000848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBH1521753.1"
                     /translation="MEEKFYIILTKIGREKIANATALGELVGLTKFQVGDSNGEYYEP
                     TEEQTALKNVVWEGNINSLRIDEKNPNWIVIETILPGTVGGFMIREAAVLDNENNIIA
                     IGKYPETYKPRAEDGSIKDLVVKMILQLSNTSNVTLEVDPTLVFVTQKDIQDLDDKFD
                     KNIKEIKVNIGDVNILTTYSKDLSGAINEVVKKIENISFDDVISGQIQTDISVLKNSY
                     NKLSEKVLDILIYLELESEVTVDEAGYWYDTLANGNNIVAIEGLKLDLNRKCITGEIG
                     NVIFRDVVLPFSANRVRYIHDMDNNFVETKSSNTYLKEQKDITLNKYSYEIR"
     gene            complement(7280..7978)
                     /locus_tag="KRL67_000849"
     CDS             complement(7280..7978)
                     /locus_tag="KRL67_000849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2313 domain-containing protein"
                     /protein_id="HBH1521754.1"
                     /translation="MKLIDKLPSFDRNYIVEEIQGAYDTELNILKEDIDDTFNQLFVD
                     TATWGLDMWEDILCIEKKELDFDTRRSNIKAKMRSRGTSTIEVIKSICEAYTKSETDI
                     KVYSDEFTFVLSFIANNCDYKTLLDCSDMIERVKPAHLLHYLEPIILDKSMVYCGGGM
                     VCSEEVKVHPYFEPIIKCSAVVNCGAGMLSREEIKVYPLSIKCIENNCKINIAIANDT
                     GVENVVVYPKSEVV"
     gene            complement(7975..9027)
                     /locus_tag="KRL67_000850"
     CDS             complement(7975..9027)
                     /locus_tag="KRL67_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HBH1521755.1"
                     /translation="MYSDQTYEVIKNRTLENINLDIYKGEGSFLNNMVSGNNLELSKI
                     YLELSKIHKMAFIQDTYNQFLDKRVNEFGVYRKLGTESNGEVEFIGEKGTVINNGTVI
                     SYRDLLFVVIKDVTIGSEEGDNSLVQALEVGKKYNLPTNCEFKLVDNISGVTKITNTR
                     SFEGGTDIETDEELKERFYKIQRNQATSGNKAHYEEWALEVDGVYNVKVYPRWDGPGT
                     VKVLIFGKNNQAVDTETIARCQQHIDEEKPIGPTITVVTPLPIEINISAVMKLEDGYT
                     LDNVKESFLESINTYFRDIRGEIIYTKVMGILINTTGVHDLSNLLINGSTDNITINED
                     KIPSVTTVNFSEVENQ"
     gene            complement(9020..9448)
                     /locus_tag="KRL67_000851"
     CDS             complement(9020..9448)
                     /locus_tag="KRL67_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2634 domain-containing protein"
                     /protein_id="HBH1521756.1"
                     /translation="MSTIFPFIGVPEDYILPKTEELPIFREVAWDFEKDEPILEKGDF
                     KIIEKKEALKVWIYKCIKTNRYEHEIYSLEYGTELSELIGQKYTKGLTESEASRFIKE
                     ALLINPYILEVNVKSANFNRDVLSANVKVSTIYGEVEINV"
     gene            complement(9448..9774)
                     /locus_tag="KRL67_000852"
     CDS             complement(9448..9774)
                     /locus_tag="KRL67_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2577 domain-containing protein"
                     /protein_id="HBH1521757.1"
                     /translation="MANPINEFIGIIREEGKHHNQPSFFIGKIKSKLPDLKIETNNII
                     LEKEDILIDSWMLDRQLETFDTETNQEHQHEVKNPFIDNFESGDMVIMFRIGEKFAVV
                     SKLVSL"
     gene            complement(9790..11322)
                     /locus_tag="KRL67_000853"
     CDS             complement(9790..11322)
                     /locus_tag="KRL67_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HBH1521758.1"
                     /translation="MIINRSKDSSSNEISFVSKDMGFLLTQSEVSYNFKDKLVEDIAK
                     QVFAENRLSVGTIAKTNVKYTKMFIGVNGYDTIMSAYTEASKKTKKKYMIEANLDKFN
                     VIEKGTVTLSVMFEEGFNIINTTFSESMENVKNKVIVVDQYGSKISEKIDNEIFKEVN
                     VIMQKVIQQQENQDVDIDSEFNGIEKSCSLKGYGDVSCITGRGVKVKDSYTKLVGLFY
                     IDTDKHTWQNGEYQIELELNFQNLMDEKSAGQDEPKEESNLGGEDYVGGTEFSAIFTA
                     YYPGPGIEGGDTDCREKKLNPSKKTCAAPMVGAYEKSYYTKEFLSKHPLFKYGDEVSI
                     VTGVSGRDGVYKVNDNGSAIIIEKDGTYHIDVLVKNAEEMKRFGKRKGKIIIGGYSGN
                     ASNKAKIVISEAKKHLGKPYKWGGNGPSSFDCSGLMVYCFKKVNVSLPRTSNQQSKKG
                     KKVEQKNLQAGDLVFFHNPVSHVGLYIGNGEFLHAPQTGDVVKISKLSSRRDFNTARR
                     VL"
     gene            complement(11604..12026)
                     /locus_tag="KRL67_000854"
     CDS             complement(11604..12026)
                     /locus_tag="KRL67_000854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH1521759.1"
                     /translation="MEMWLRQAEDRFRFPVFPSSFSINGKAAVNSSSILKIGEIATFG
                     GVALKSISISSFFPNKDYTFCDYTGFPSPYDCVNKIEKWMKEGFILRFTITETNINME
                     VIIEGFSYEERDGTRDVYFTLDLKEYKRIKIPKVTPKQ"
     gene            complement(12026..14425)
                     /locus_tag="KRL67_000855"
     CDS             complement(12026..14425)
                     /locus_tag="KRL67_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH1521760.1"
                     /translation="MGNVREEGINMYLTDNYTPKMNQIISVTDNFRRATVAVSLSTNV
                     MASSIKNSIGSASSRVNSLNSSLRKVQTTASSVSSTMTKLSSSINAVSGVIGSLNGSI
                     MRLAITIAMIIDYFNKLIQKKNEFNSNIMIILIFKAKSDEIEKTKNKLLGNLKKIGGK
                     VWKIVIKAKDMTKRVISSILGKLKQVEKRPYQGSINLKDMVSSAMGRILPKLMLFKNT
                     FWSGVIAIKDMASGIISKVFPKLRLFAGKVWSGAIAVKDMASGILGSIKGKISDLTNG
                     ATIGVAVKKGVDLLGQEQNQKVVLESVMKRNTGKVNQREVDDYYGSLVRMANDTPFDP
                     EDVVAMGTKAKMISNITGGKKEKDITQAMVDVRALNMNTSSEQDVSAAFLSAAKGNME
                     SLNTLVGENYKTFDEALEGISVKQMGLAKEMSNTIPGIISGAQTSINNGLKSIVKPFD
                     DILGQGLKKIKTFIESGLGNLAGLSERMAGKIGNVMNGKIIIGNKYDQMQSRSVKNGK
                     EFSDSTQYRISNEAEKRKMMVENKQERFENHAATMIGNAPKAIVNAGSTLLQNIDFTA
                     LIDSLLPVVNLVNNLLDSINNKSPIAQGLISIFGTIVTTAFQLIGPVVEAVSPIITRI
                     FTFLGEYAPQINNFIETLGVIWETVWATIGPLLEDAWIIVEPVLGGLFNLFNDICNII
                     KDVCKWWQNLVEKINKNPIIETVRSIIDDWNAGTSKSGSNAFGLNYVPYNDYPTRLHE
                     GEMVLTKQEANQYRSGKNGGNISIAKLADTIVIREEADIEKVTSKLVSSIKMAQLGGV
                     L"
     gene            complement(14425..14592)
                     /locus_tag="KRL67_000856"
     CDS             complement(14425..14592)
                     /locus_tag="KRL67_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH1521761.1"
                     /translation="MYYYWKKKGIRPSLFYAMDKGELKLIEAFFALEIEEEVEKMKHG
                     YGMCPLTGGGM"
     gene            complement(14619..15065)
                     /locus_tag="KRL67_000857"
     CDS             complement(14619..15065)
                     /locus_tag="KRL67_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HBH1521762.1"
                     /translation="MSENGLSENINIVDLLLNSDTENLERPSTIVELKRLSTIFGQEF
                     KVMCRALTISKDEEIQNTCLKIDENMKTDIDLPEMQMLTIIEGVCDLDGKLLFKNKEL
                     MDKFKAPTPKELARKLLLPGEITNLYRILQDVMGYGKNAVIEEVKN"
     gene            complement(15179..15607)
                     /locus_tag="KRL67_000858"
     CDS             complement(15179..15607)
                     /locus_tag="KRL67_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tube protein"
                     /protein_id="HBH1521763.1"
                     /translation="MANMEARNVMSGTWGELWLDGNKVAEVKKFQAKMEFTKEDIIIA
                     GQMGTDTKYMGYKGKGSITLYHVSSRMHKLIGEKIKRGSEPRFVAISKLNDPDSYGAE
                     RIAVKNIAFDDLTLADWEVGVKGEIEAPFTFTEYDFLDII"
     gene            complement(15621..16685)
                     /locus_tag="KRL67_000859"
     CDS             complement(15621..16685)
                     /locus_tag="KRL67_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath subtilisin-like
                     domain-containing protein"
                     /protein_id="HBH1521764.1"
                     /translation="MAIGLPSINISFKELATTVKERSARGIIAMVLKDAKALGLNEIH
                     EKEDIPVDLSAENKEYINLALMGNVNTPNKLLVYVIEGDSDIQTALDFLETKEFNYLC
                     MPKAVEADKTAIKNWIIKLRDIDKVKVKAVLGKVVGNHEGIINFTTEDVLVGEKKYSV
                     DEFTSRVAGLIAGTPLSQSVTYTKLIDVVDIPKMTKVDAESRVNKGELILIKEAGAIR
                     IARGVNSLTELTEEKGEMFQKIKIVDTLDIIHSDIRKVIIDDYIGKVTNSYDNKCLLI
                     VAIKSYLEELEKSALIESDSTVEIDFEAQKSYLKSKGVDLSYMTLQEIKEANTGSKVF
                     LKAKIKVLDAMEDIDLSIEI"
     gene            complement(16690..17133)
                     /locus_tag="KRL67_000860"
     CDS             complement(16690..17133)
                     /locus_tag="KRL67_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH1521765.1"
                     /translation="MLKYKEILETIIEILKKNFTESIFIDDESVQGSEGSCFFVSILS
                     VICTPIMLNTNNKDIVISIKYLPKPQSNSIRMYEISDELNKLFNRNIKVTDRKLNITK
                     LEQSIKKEESIYALNFSVTLNYLDSVYEEDVVYENMEEINLNLGE"
     gene            complement(17382..17822)
                     /locus_tag="KRL67_000861"
     CDS             complement(17382..17822)
                     /locus_tag="KRL67_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH1521766.1"
                     /translation="MENKKDILFKETDKRLHNYKYLDIKIKNINLDIKRCENEYSGCG
                     AMVYTEKTSNTYNISSSVENEVLKREERLRKLKMEKEDIEIEKEKIENALTCLNDIEM
                     EFFNLFYNSKTKNNMTYISMKLHLDRTSCYNLKKKMIFKLSEIL"
     gene            complement(17927..18124)
                     /locus_tag="KRL67_000862"
     CDS             complement(17927..18124)
                     /locus_tag="KRL67_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBH1521767.1"
                     /translation="MFKNNLKYYRKCKGMTQIQLARKAGITNDYISQIERGIKNPGLL
                     MAKKISSILEQNIEEVFFIQL"
     gene            18293..18613
                     /locus_tag="KRL67_000863"
     CDS             18293..18613
                     /locus_tag="KRL67_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HBH1521768.1"
                     /translation="MFAKRLRELRKEFGLTQRELGEKVGVSQRVLGYYETENRFPDEH
                     ILNKLADVFNVSVDYLLGRTLVKENIDTVAAHRKNPHEELPEEAQEQLNDYIEFLLNK
                     YKKK"
     gene            18695..19201
                     /locus_tag="KRL67_000864"
     CDS             18695..19201
                     /locus_tag="KRL67_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH1521769.1"
                     /translation="MNNLDKLFELASQEEIIIHYTTYIAGDLEGLYINKHGIKIISLL
                     SNLKQNSKKLTSILAEELGHHFTSLGYYVSSYNDYYTKIIIDKCENKALKWACEFLIT
                     EEDIINIINSGITCVYEMADILNVDITFFQKRLEFLSLKKQSLQLGNNKYLILTNLPY
                     FYIFDPIS"
     gene            19365..19781
                     /locus_tag="KRL67_000865"
     CDS             19365..19781
                     /locus_tag="KRL67_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH1521770.1"
                     /translation="MYKEKLFDNYFKFLALLFWPIMWYKWIVISNGTLENMLFTIYAI
                     IAIVFIILYSVFMIKYKDITQIDFFYRISTLLAFIFTLFSFLIYPKSLFFLYLKIIFT
                     GIYLYYSIVKTLKFKDDEGVVGIMSSLLLIVITLFY"
     gene            19902..20657
                     /locus_tag="KRL67_000866"
     CDS             19902..20657
                     /locus_tag="KRL67_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HBH1521771.1"
                     /translation="MEKKVLATVGEKEITNIDIENALKSLDPYQAMQFKTEEGKKHLL
                     NDLVNQELFFLDAKEEKLDEEEIFKLEMKKIEENVLKQFAINKVLSSVNVTEDEKVKF
                     FEANKSSFSKPESATAKHILVDSDEKAKEILAQIKSEEISFEDAALKHSSCPSKDMGG
                     DLGTFGRGQMVPEFEEAVFSMAKGEVSEPVKTQFGYHIIKLENLQESTESTFDEVKAE
                     VEKSLLYQKQNEVYGNKINALNAKYGNLVKYND"
     gene            complement(20800..21228)
                     /locus_tag="KRL67_000867"
     CDS             complement(20800..21228)
                     /locus_tag="KRL67_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HBH1521772.1"
                     /translation="MNILFFLTPKSEVAYIYEDYTIRQALEKMEYHKYSAIPIISKDG
                     KYVGTITEGDFLWTLKNDLNLDLKGLEDVPVTDINRKMDNSPVSINADIEDLVIKSLN
                     QNFIPVIDDQDTFIGIIKRRDVIGYCYEIIRGYKNLANDN"
     gene            complement(21649..21849)
                     /locus_tag="KRL67_000868"
     CDS             complement(21649..21849)
                     /locus_tag="KRL67_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HBH1521773.1"
                     /translation="MKTGVVKWFNNEKGFGFISVEGEGDVFVHFSAITGDGYKTLEEG
                     QSVEFEVVDGAKGPQAANVVRL"
     gene            complement(22032..23201)
                     /locus_tag="KRL67_000869"
     CDS             complement(22032..23201)
                     /locus_tag="KRL67_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase domain-containing protein"
                     /protein_id="HBH1521774.1"
                     /translation="MGGVALKILKNKNKYIKGIMAFVFLSLATTAGVFLINDLSMKNM
                     EEKVMESANVEGDDVKDQYKLLLENLFDYRNKAILEKNENILKGLYETDKKFGLWAYE
                     NEVKKMKYLENWSSKQGVKFKDIKTRVKIKKIKEKEKDLYGIICSVSTEYKYSYENQK
                     DKENAFRIGTYHYLNVKIIDNQYVITKEWYTDPFADSLNLENIKSDDIRNYIGQQDGV
                     KLQLTDEQKKAIDYAHKYCGAAADEEHGMKFNPNYRDYNPEGGDCANFASQIMFESGR
                     FKKNSIWNYSERAGTKAWVNAQAFKNYALYSGRGSLIAKGSYEEVYKEAYNLRPGDFV
                     GYEKKGRITHVSTVTGLDSRGYPLVTCHNTDRMLVPWDLGWSDKSIRFHIIKINY"
     gene            23437..24258
                     /locus_tag="KRL67_000870"
     CDS             23437..24258
                     /locus_tag="KRL67_000870"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine kinase"
                     /protein_id="HBH1521775.1"
                     /translation="MQKKVAAINDLSGIGKCSLSVAIPILSALKVQCCPFPTAILSSQ
                     TGYPEFTFLDFTDEMVKYSNVWKNLKVNFDSIYSGFLGSKHQIEIVANFINDYPNAFI
                     VVDPVMGDNGVMYPIFTEEMRQEIKELVKHSDLTTPNLTEACFLTGNDYTKSDYNRDE
                     LIYIAKSVSDLGPSKVVITGILEDDNILNLAYDRDNDHVFFTSVKYNNCSYSGTGDIF
                     TSILCGMLVNKHDLGVAVNTATDFIYKTINYTSQFDTDRNDGVMFENFLSDLTNI"
     gene            complement(24334..25722)
                     /locus_tag="KRL67_000871"
     CDS             complement(24334..25722)
                     /locus_tag="KRL67_000871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase"
                     /protein_id="HBH1521776.1"
                     /translation="MLGGKKSNNKKVEKLRTIFIKYLSLFFIMTISIVLILILLFSWL
                     LSSGVILPANYAEKQINRYKEKIISSEKVTEDIIPNIYEYGVYTLDGNLISGTFNKKE
                     SKEVWNLMRDIEERHAYSESYIKFFKKDEVFIIKYKIVSEYSSPVLRAYLPKPETLGM
                     IIFSIIFFIEIVILSKVFGKKFNIEMELLKNTTEKIEQQDLDFVVESSKIYEINNVLF
                     SMDKMKLALKDSLEKQWMLEENRKEQISALAHDIKTPLTIIHGNTDLLIEINENPELL
                     EYIEYISKGVTQIEKYINTLIDISKTENGYILNKEVINMSEFIKDILVQIEALASTKN
                     LDVEFYKQDNLPKNITIDRELLFRAIMNIISNAIDYSPSQSKLYISVSVSNKCLKFVL
                     TDCGSGFSKADLSKATEQFYTGDLSRNSKSHYGMGLYIVNNIVQKHNGTLYIENSVKT
                     GGAMVTIEIPIV"
     gene            complement(25857..26615)
                     /locus_tag="KRL67_000872"
     CDS             complement(25857..26615)
                     /locus_tag="KRL67_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lantibiotic immunity ABC transporter MutG family
                     permease subunit"
                     /protein_id="HBH1521777.1"
                     /translation="MRQLIRIFCSDLIKLKRNFIILMHICIALMGMGLFLWYYKVSGA
                     DNILKIVAYLQVIAIAFPLLSGVVCSMFVEQEYYAGNYKHMLTTSNPKYLTLISKYVI
                     LVCLGFVATLVSVLGFKFGISEAYFTSNFYMISILILIGCSLFEYILHLFLSLRFGKG
                     ASIGVGIVEMLLSALLLTGLGSGIWQYFPCAWGVRFITIWSSFSSSKTVEYIKVESIK
                     AYQSTGLMCGFVTILAFVILCIWFSKWEGKKSEE"
     gene            complement(26617..27333)
                     /locus_tag="KRL67_000873"
     CDS             complement(26617..27333)
                     /locus_tag="KRL67_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lantibiotic immunity ABC transporter MutE/EpiE
                     family permease subunit"
                     /protein_id="HBH1521778.1"
                     /translation="MERFIRVEFQKMKGTFGMKLIWIVPVFCILFCGLGGGQNAAFNW
                     WYTTFLPGALTLICSLIVLKDEKSKYRGILPLPIEMKDVWTGKMIYCIICCFIASIIS
                     AIGVVFVGIIYKETISISQNIIATILIILLFMWQIPLCMFLASKFGIFVTVLTNMFFT
                     IVGVAVFSTTEMWYIFPYSIPSRLMAVVLHILPNGLPVPEGSELLNNAVILPGVLISI
                     VLFVLFALATRVWFNSREVK"
     gene            complement(27335..28042)
                     /locus_tag="KRL67_000874"
     CDS             complement(27335..28042)
                     /locus_tag="KRL67_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lantibiotic protection ABC transporter
                     ATP-binding protein"
                     /protein_id="HBH1521779.1"
                     /translation="MSNYILQVKGVSKKFKEQMAVDNISLAIKPNSIYGLLGPNGAGK
                     STLLKMITGILNPTSGKIMFEGHRWSRKDLSNIGSLIESPAIYENLTARENLKVHTTL
                     LGLPDSRIEEVLEIVDLRDTFKKRSGQFSLGMKQRLGIAIALLNHPKLLILDEPTNGL
                     DPIGIEELRELIRSFPKQGITVILSSHILSEVKQVVDEIGIISNGVMGYQGEVSKEQN
                     LEELFMKVVAENRKRGE"
     gene            complement(28158..28694)
                     /locus_tag="KRL67_000875"
     CDS             complement(28158..28694)
                     /locus_tag="KRL67_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HBH1521780.1"
                     /translation="MNKIAVSFLIIATTLLSTACMDYSISAVELVDSKESAVVKKDED
                     AKEETTSKMINSKKTTKIPIEIISKDEKIVKYLQIDEESSLKDKLRLILDTLSNEYFN
                     GLDMEVEVKEKDNLVKINLIEPDKKSRVSWKDDYLNEQNIIYTINNIIKNVIQEEDNS
                     IWIEEVEIYYNGKLIELR"
     gene            complement(28796..29866)
                     /locus_tag="KRL67_000876"
     CDS             complement(28796..29866)
                     /locus_tag="KRL67_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat protein"
                     /protein_id="HBH1521781.1"
                     /translation="MNKKLAIFAAVLLVIGIIGTTWSGILVMPSLINFGLEKEAEFKK
                     ENKLYQEKVNIDKLDISVDNINVTIKKSSSEDVRVTTRGNNEFYKYNVTLKDKTLVVK
                     GERKYENKIKKIKNFDQLLNTSINSMFSHDYREIIIYVPNNVDINASSISSYLFVYDN
                     VASNNITYKTSSGGFSRAITENKVNRLENLNLISNSNLHLSTKSILGVKNVNIESESL
                     DISSENEDVFIDNIEEYIPENVNIKEKIGRNSNYDSEFYLYSNMPIAKFLDIEVPNSK
                     VRLDIPVNKYKFNCDIKSKEVIEEFNNDEEYDNDSYDYEHSEEHSNKYRNTREIKGLL
                     NKNLSNLEKEYTIKINSNSLEL"
     gene            complement(29859..30917)
                     /locus_tag="KRL67_000877"
     CDS             complement(29859..30917)
                     /locus_tag="KRL67_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1700 domain-containing protein"
                     /protein_id="HBH1521782.1"
                     /translation="MNLNKTEFLEILKDYLKKDFSESEVNDILRDYEEYFVDGLIEGK
                     SDMEIIASLGSPKSIAKELILQTKEKNIEEEKIINTDVLNDKFSKFKSDMKKGYSILK
                     DEAKEKYSKSKENIDGKIMNTDILNGKFSRFKSNIKKDYSILKDEAKEKYNKSKENIN
                     DKLTPSIEEGVGGLSRQVIKGLLVVLSLFLIVPAFAFVCAIVSVTIALVCSLIAFVIL
                     IPFVIKFIAMVPDVMMFCIFATIAFVGIEILAWQILLFAIKLGKQLLNNYIHWIKRKN
                     IYIKANEKMEKRKKATLNDEDLNKDINEDSYNNDYYNREEESIKETDEYNDDSINFEA
                     IYKDLEDIDNKKGEDKHE"
     gene            complement(30904..31236)
                     /locus_tag="KRL67_000878"
     CDS             complement(30904..31236)
                     /locus_tag="KRL67_000878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HBH1521783.1"
                     /translation="MNTQFRKGVLEICVLALISKKDMYGYEIVHNISKVIDVNEGTIY
                     PILRRLTKDLYFETYILESNEGPARKYYRITSLGKENLSNLMEEWREFVKAVEILISE
                     DEGGNELE"
     gene            31435..32556
                     /locus_tag="KRL67_000879"
     CDS             31435..32556
                     /locus_tag="KRL67_000879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactosyldiacylglycerol synthase"
                     /protein_id="HBH1521784.1"
                     /translation="MKDDKTILILTAQFGAGHISAAKAVKECIIEKYSNYNVVIQNFI
                     NASIPMMNKPMVKLYENNTKYTPGLYNYYYYFKKSFDSRHDLSHKLYTPKLSEYIADI
                     NPDLIISTFPLAAACVNNFKIKNPDINIPTLTVITDVVDSMEWVFENTDLYFVPSPEI
                     KNRFFQKGINPDSIKVTGVPVDKRFQIESKELCCDKYRLLLLGGGRGLFDIDEDFMHW
                     IDEFIEEHSNSIEITIVTGKNKKLYDNLTHKKPLKNIKVLGFVNDMYNLIRECDLMLT
                     KPGGATIFEAIQSQTPVLVKMPKVGQEIENAKFIIDKGLGMIYSDDLDLKNIFYKLVS
                     NEFDSIINFMKKNLEEFKTVIHPEKIADYISELIDNHYS"
     gene            complement(32684..34582)
                     /locus_tag="KRL67_000880"
     CDS             complement(32684..34582)
                     /locus_tag="KRL67_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase"
                     /protein_id="HBH1521785.1"
                     /translation="MDSLLYVIEKDKHTNEELREILKSNKNIRFVSLMGVDLGGNATD
                     EKIPVELFLEDIDKFLESAIQTDGSSVELYNIATLNNAKVDLMPDKSCHWYVDYNMEY
                     IDEEVGLPVGTLKIPAFLIHDNKKVCSRGVLQKADKYFKKSMYEIFREYPHVINNIGI
                     DSVDDIEEIMLTAATELEFWVNTPEDKADLEKLYVSQSLKEQYWKRTHGIIRTCLEKS
                     LIILQKLGVNPEMAHKEVGGIQSSISIDGRTNHAMEQLEVSWKFSTPLQAADNELLVR
                     DVIEDVFTSHGLEVTFKAKPIHGVAGSGGHTHVGVSAKLKDGSIKNLFAPKDLKEDYL
                     SELGYGALMGLLYNYEVLNPIVTASNDGFNRLVPGFEAPVCIVTSLGHSYEIPSRNRS
                     VLVGLIRDMKNPKTVRFELRSPSPLSNTYLVIAGCYQTMLDGIKAAAKSGLSTKELEK
                     ELSKNVGEESFYLEKDRAYRDENDVFEHYSLEERNARFGIPPATVYENMKNLEIYASK
                     LKSLKQGDVFTDSIIESFKIGAIDKWQKKLKTRIIEAGIQKIRSIVKIHTKENMDALD
                     EVVWNSISDLKFNIMKDTLTRESLFTRVREAIENKDYQTASDLQIELKRSMEEIQQLY
                     MQYKKNIY"
BASE COUNT        11128 a         6047 c         3700 g        13985 t
ORIGIN      
        1 atgttatttt tatatattat ttttattact ttttttaatt aatattttta aatattaaat
       61 ccttctcaat aaaatagggt actatctaca atactacttt atcttttcct aacaatatat
      121 aatctggtac tactgctact ttcatgttat acaccgtttt gtaacatacc ttttatatca
      181 gtaacatctt ccttaatttc ctctacatct gttttcatta ttcccatttg aacaagtata
      241 ttcttattta tttcttgttg ttgtgtagat aattctataa agttttctac agttttttta
      301 tacatgtctc tatcttcttt tttctcctgc atagtatttt taaataatag agcacatagt
      361 atacctattg cccctagact actcaattcg gttattaatt tatccattat agcctcctat
      421 tgtctaaatt ttttatactt atagctttaa tttttgttat attcttacat catgcaatat
      481 tatattttca ggattatcaa tttaatcttc tgtactttaa attgtaggtg taatttatat
      541 attgaataaa taataaatat atatacaatt tctaatataa caaaaaataa gtacaaggct
      601 tacttattaa agttaagacc ttgtacttta cataaaaaaa ataactaata catagttatt
      661 ctaaatttac tattcaactg gtatttctaa ttctttacat ctaattctaa cttgctctct
      721 aattactgct ggtacttctt ctattgtttt tttgcctctt aaaatcaaag ctacatatac
      781 ttctaccatt ccactctcac ctccagattt tcttacacta aaagtgctta tattacttgc
      841 ttcattgaca gaactttgac ttaacaacat ctcatatatt tctgcatttg ctataaaatt
      901 atttgttata tcatcatctt gctttacttg acctatactt atgtctgaga tatctttata
      961 ctgcatttct acttttctac cttgtttgat ttgttctatt ttatttttat cgaatttaaa
     1021 caattttcac acctccattt taagcttgtg gctcgttgtt ttcagcatca tatctaaaaa
     1081 tcatagatgg acgttttata gctgtccatt tatctccttt ttctactatg attttaggct
     1141 gagcactttt tatgttggct tcttctaatt catagatttc ttggtaactt gagtctgatt
     1201 ttttgatttc aactagtttc atgtctttga ataagtcaga tttatttatt tttacttgaa
     1261 ctttagtttt atttattaat tgatatatag ggtctccaag atttaaatct tcttcaagtc
     1321 ctctatctag tgtaattgta atatcttgtt cattggatgt tgtattaact accttgtaca
     1381 ttctattacc tatcataaca taagaatctt taattataga atttccagtg ctctctttaa
     1441 aaataaataa agatgcatat aaatcatcac ctttacaata agctctaaat aatatcttat
     1501 tctctcctat tttaatttta tctggtggta cttcaaatga tattttttca gtaattaaat
     1561 cacttacaga ttttacactt tcaccattta taagcatttc tattctactt atatctttat
     1621 tagggtcttg tatttttatc ttaattgatg aaattgattt ttcagtaatt tctatagcag
     1681 catttgtcgt tcctacttct gatataatat ataatgtatt gtctgttgca agtacatcct
     1741 ttactgtatt tacatcttca gaaataggta ttatagtaaa ttcttttaaa aattctactg
     1801 gttgttcaat tttatataca tccctatttt ttccagtaac ccatactgaa ttatcatttt
     1861 taagtactaa tatatggtca cttccataag attttatttc tcttatgtct gttagtgctt
     1921 ttgtaaagaa cttaattata gtattttcac tatcgcttcc taatcctaaa gctcccttat
     1981 cattatatcc ttgtacaaat aattcattac ctgtagttac tactaatgta tagtttttac
     2041 cacaatgtac ttctttacag ctagatatag ttgtaattct ttgtaattca gtaactggat
     2101 tattgtgacc tatacctaat tgatattcgg aatttttacc aatagaccaa agactatcat
     2161 cagattttat aaagtaagta aagttatctc cacatgaaac atgctttaca tctgatacat
     2221 taatctgcac aaattcatcc acttgattta tagacaagtc ttgcaataat tctccatggt
     2281 agtttgtacc acatccatac attgttccat cattaagtaa tataataata tgagtcttag
     2341 aaatagatac ttcttttata tcttcatatg gaagctcagt ataaaggtct cctttgtata
     2401 aatttcctaa tactaaacca taatctatgt cttttgtggt aggtatcact atcttattgt
     2461 ttagaagtat aaagttatgt ctgtttttaa tagatgtcaa atcacccatt acaaaattca
     2521 tatgcttata attaaatatt ctattatcat tatttctact atcgtatcta tttccaacac
     2581 ctaattgtcc atagttattt aatccacaaa tatatatatc attagtatct tttattgcta
     2641 tatttgcata ttctaaagga agtattttct ttactcttgt attggttttt ataaatgtat
     2701 ctctttttgt tacatcacca acacctaatt gtccattatt attaaaacct gtaccatata
     2761 ggtctccatt atcacttaca taatgtccac atctataatt cattccaatg tcctttgcct
     2821 ttgatgctat ttttttaatc ttctctggag tctcacttcc tgtacctgaa ccattactac
     2881 atttacctgc agtatataca tccccttcac ttgtaagtaa aagtactgca ttatttctgt
     2941 ttggagctac ttttactaca tttgaaattg gaatttcaga aaatgaactt tgattactat
     3001 tactatttag acctaaacaa taatcagtat ttagacctgt actatatgca gtattataga
     3061 ttgttattat ataggtattt atttcatttg gtataacttc ttttacattt tccatacact
     3121 ttataaaact atactggtca gtagtatttc ccatacctaa ttgtgaatta ttattgtatc
     3181 cacaacaata tgcaatgttt gtgtccttta caataaatgt tgttgtatct gagcagtata
     3241 catctcttac attagttata ttgtttacag taaatgtcaa tacgtctgga tggttgcttt
     3301 cgcttcctaa tcccagttgc ccatgagtat tttctccaca tgaatataat gtgttatcat
     3361 ctttgattgc atgtgagtga gtagtcccta aaacaacatc tcttacattt tgtatattta
     3421 ctttggtaaa tgtgtttcta tttactatat cacctaatcc cagttgtcct ttggtattta
     3481 atcctgtgga gtatagtgtt ccatcatttc taattgcaaa tgttgtgtta ccatctatac
     3541 ttgttgatat tttctttata ttatcaatgt ttatctgagt gaacttattt ctatttattg
     3601 tgtcacctaa tcctaattgt ccagaactat tatttcctgt ggcataggct gttccatcat
     3661 tttttactat aaacattgta tttcttgatg ttgtaatttc ttttatatca tccataaata
     3721 taaatgagtt tgattgttca gttatatttg tgaatttttt tattggttga ttgttatatt
     3781 ctcctatacc aagtttgtta aattgatttg ttcctgttac atatatattg ttgtttttat
     3841 ctactactac tacagtgtta ttttttacaa ttatatcttt tatatctttt aatcctgtat
     3901 cataattgct tgcattactt atactagttg aactcccaaa ctctccatta ttagattttc
     3961 catatccttt taatacactt tctccagaat aattttttag tatgaactca tttcctaatg
     4021 atactgcacc attacagtta tcgctcaaat aactagacca attttgatta gtttgtccat
     4081 atgttaaaca acttcctgtg cacctaaatg tagaattatt ttttaataac gataaaatat
     4141 ttttactatt tcctacaaca ctttttgaac tatcaaatac tgaacaacga attagtttat
     4201 atgtccactg atagcaagta tatactcctc catctgaatt tgtatgatat atgcctgaat
     4261 tactactaaa tcttaattca gattctggag ttatttgaga agaacttgta aatttcatat
     4321 tattttcacc aatacaataa gctcggtcac tttcatcaaa aaacatacat ccacgataaa
     4381 aagcaaataa atttattaat ttagtttgag ctgtattata aaattccaat gttctatatg
     4441 acgtattcca agcattatat cctttactct gatatgatga actagtacta ggtgctggat
     4501 atccaccact accattatat gaataaccta tagtctttgc agcaccagtt gaatctaata
     4561 tatataaagt atcactccac gtagctattt tttctacatt actaactcta gtatctttgt
     4621 aagggtagtt tctattatca ccataaacta tataagattg attaccccac ccatataatt
     4681 cctttgaatg atttaatgct acaatatagt tactttctac acaaaattcc ttaatatttt
     4741 ctatctcaat ttttgtaaat atatttctat ttgtattgtc ctttaatccc aatataccag
     4801 aactatttaa accacaagaa tataaagttc cattattctt aagaacaaaa gtcgagcttc
     4861 catttccttt tatatccaaa acattatcta tatccacctt agtcataact gacctgttaa
     4921 cagtgtctcc caatcctaac tgcccagcat tatttagtcc acaggcatat aatgacccat
     4981 cattcataac aataaacata ttattatctg ttaactctat cttcttataa tcctgaaaat
     5041 tatcacttaa ctttacattt aaaaattcac tattatcctt tgtaacatat aaataatcat
     5101 cattaaccaa taaacctgaa taattatcta aatttgaaga ttgtaccttt gaaaactcat
     5161 acagaaaagt tgtatgaaaa aatgaaccca gttgacctgc taaattacag ccagatacaa
     5221 atatctcttt tgaaccattt attaaaaaat taaattgaga accacactta atatctttta
     5281 tattttctac attaacctta gcaaaagtat ttcttgatgc tacatcttta ttggcattgg
     5341 ctagttgacc ataattgttt tgtcctgtac taaatagtgt attatcatat tttaatatca
     5401 tagtatgatt atttccacaa gaagcatact taacattatt tatactagaa agagtaaata
     5461 caattgaatt attattattt cctgtaccaa gctgaccaac tccattatag cctgtagtat
     5521 acatagttcc atcattctta ataagaattg tatgactact accacacgct atctgtttta
     5581 cattatccat accttctatt ttattaaata cacttttatt taaagtacta ttaattccca
     5641 actgcccatt tgaatttaat ccacatgcat gtgctgtccc atccattttt atgataaata
     5701 cagattgatt ataagttact acatccttta cactatctat atttacttta gtaaaagtag
     5761 ctctattgtt agtatcacct agtccaagtt gaccacttga gtttaaacct gctgaatata
     5821 atgttccatc atttttaagt acaaaagtaa agtcataacc acatgcaact gtttttacat
     5881 catctattgg tagcttagta aattcataat atactgtaga ctcaattaca cctaattgac
     5941 ctacattact tgttcctgct ccatatgcaa atccatcttt agttactgct acactgtgtt
     6001 ttgaaccaca agatacatat tttacattat caatttttac tttactaaac atccttctct
     6061 ctgacgaatc ttttccaagt cctaattgaa agcctgtatt atcaccacaa gcatataaat
     6121 tcccatctga ttttaaacca aaggtactgt tacttatacc agtaaaaaat tctacaaaat
     6181 cataatcatt atatctttta tcaaaatcat caatcaatat gttcttatca ttaaaataag
     6241 caccacgttg taaaagttta ttttgcttca ttattagtac tacccccttt atttatctta
     6301 tttcatatga atacttattt agagttatat ctttttgttc ttttaaataa gtgttgctag
     6361 attttgtctc aacaaagtta ttatccatat catgtatata cctaactcta tttgcactaa
     6421 atggtaatac tacatctcta aaaatcacat taccaatttc acctgttata cattttctat
     6481 ttaaatctaa cttaagccct tctatagcta ctatgttatt tccatttgct aatgtatcat
     6541 accaataacc agcctcatct acagttactt ctgactctaa ttctaggtat attaatatat
     6601 ctagcacttt ttcagataat ttgttatagc tattttttaa tactgatata tcagtttgta
     6661 tttgaccact tataacatca tcaaaagata tattttctat ttttttaact acctcattta
     6721 tggctcctga taaatctttg gaatatgtag ttaatatatt tacatcacca atgtttactt
     6781 ttatttcttt tatattttta tcaaacttat catctaaatc ttgaatatcc ttttgagtta
     6841 caaaaaccaa cgtcgggtct acttctaatg taacatttga agtattggat aactgtaaaa
     6901 tcatttttac aaccaaatct ttaatactgc catcttcagc acgtggctta tacgtctctg
     6961 ggtacttacc tatagctatt atattattct cattatccag aacagcagct tctcttatca
     7021 taaatccacc aactgttcct ggtaaaatag tctctataac tatccaatta ggattttttt
     7081 catcaattct tagagaattt atatttcctt cccaaactac attctttaaa gcagtttgtt
     7141 cctctgttgg ctcataatat tctccattac tatctccaac ttgaaacttg gttaatccaa
     7201 caagctctcc tagtgcagtt gcatttgcta ttttttctct accaattttg gttaatatta
     7261 tataaaattt ttcttccaat tataccacct ccgatttagg ataaactact acattttcta
     7321 cgcctgtatc atttgcaata gctatattaa tcttacaatt attttcaatg catttaatgc
     7381 ttaaaggata aacctttatt tcttctctac ttaacattcc agctccacag tttacaacag
     7441 cactgcattt tataattggt tcaaagtatg gatgaacttt tacctcttca ctacatacca
     7501 tacctccacc acaatagacc atacttttat ctagtattat tggttctaaa tagtgtaata
     7561 agtgagcagg ttttactctt tcaatcatat cgctacaatc taaaagagtt ttatagtcac
     7621 agttatttgc tataaaactc aatacgaatg taaattcatc actataaact tttatatctg
     7681 tttctgattt tgtatatgcc tcacatatac tttttataac ttcaatagta ctagtacctc
     7741 tgcttctcat tttagctttt atattgctac gtcttgtgtc aaaatcaagt tctttttttt
     7801 caatgcagag tatatcttcc cacatatcta atccccatgt tgcagtgtca acaaataatt
     7861 ggttaaaggt atcatcaata tcttctttaa gaatatttaa ttctgtatcg tatgcacctt
     7921 gtatctcctc tacaatgtaa tttctatcaa atgatggtag tttatcaatt agcttcattg
     7981 attttccacc tcactaaaat taacagttgt tacactaggt attttatctt cattaatagt
     8041 tatattatct gtacttccat ttataagtag gttacttaaa tcgtgtacac cagtagtatt
     8101 tataagtatt cccattactt ttgtatagat tatctctcct ctaatatctc taaagtatgt
     8161 atttatactt tctaggaaag attctttgac attgtctaat gtatatccat cttctagttt
     8221 cattactgca cttatattta tttctattgg taatggcgtc acaactgtta tagttggtcc
     8281 aataggcttc tcttcatcta tatgttgctg acaccttgca atcgtttctg tatcaacagc
     8341 ttgattattt ttcccaaata tcaagacctt aactgttcct ggaccatccc atcttggata
     8401 aaccttaaca ttatagactc catctacttc caaagcccat tcttcatagt gagctttatt
     8461 tccacttgta gcttgatttc tttggatttt ataaaatctt tcctttagct cttcatctgt
     8521 ctctatatct gtaccacctt caaaacttct tgtgttagta atctttgtta ctccagatat
     8581 attatcaact agtttaaatt cacaatttgt aggtaaatta tatttcttac caacttccag
     8641 agcttgaact aggctattgt caccttcttc actaccaata gttacatctt ttattactac
     8701 aaatagtaaa tctctatatg atattactgt gccattattt attacagttc ctttctctcc
     8761 aataaattca acttctccat ttgactctgt acctaacttt ctatatacac caaattcatt
     8821 gactctttta tcgagaaact ggttatatgt gtcttgtata aaagccattt tatgtatctt
     8881 tgaaagttct agatatatct tcgaaagttc tagattattt ccagatacca tgttgtttag
     8941 aaaagaacct tcacccttat aaatatcaag attaatattt tcaagagttc tattttttat
     9001 tacttcatat gtctggtcac tatacattta tttccacctc cccatagata gtggatactt
     9061 ttacatttgc actcaatacg tctctgttaa agttagcact ttttacgttt acttctaata
     9121 tatatggatt tattagaaga gcttctttta tgaatctact agcttcactt tctgtaagac
     9181 cttttgtata tttttgtcct attagttctg aaagctctgt cccatattct aaagagtata
     9241 tctcatgttc atatctattt gtctttatac acttgtatat ccaaactttt aaggcttctt
     9301 ttttttcaat tattttaaag tcaccttttt ctaaaatagg ttcatctttt tcaaaatccc
     9361 atgccacttc acgaaagatt ggcaattctt ctgttttagg taagatataa tcctctggga
     9421 cacctataaa aggaaatatt gtactcatta taagctcacc aacttactta caacggcaaa
     9481 tttttcgcct attctaaaca ttattaccat atccccagat tcaaagttat ctataaaagg
     9541 attttttact tcatgctggt gttcttgatt tgtttctgta tcaaatgttt ctagctgtct
     9601 atcaagcatc caactatcta tcaaaatatc ttctttttct aatatgatgt tatttgtctc
     9661 tatttttaaa tctggcaatt tactttttat ttttccaata aaaaaagaag gttgattatg
     9721 atgctttcct tcttctctta ttattcctat aaattcattt attggattag ccattatatc
     9781 accatccttt tataatactc tcctggctgt attaaaatct cttctactac ttaacttact
     9841 tattttaact acatcacccg tttgtggagc atgtaaaaat tctccattac ctatatatag
     9901 tccaacatga ctgactggat tatgaaaaaa tactaaatct cctgcttgaa gatttttttg
     9961 ttctactttc ttgccttttt tagattgttg atttgatgtt cttggtaaac taacattaac
    10021 ttttttaaaa caatagacca ttaaaccaga acagtcaaaa ctacttggtc catttccacc
    10081 ccatttataa ggtttaccta gatgtttttt tgcttctgat attactattt tagctttatt
    10141 ggatgcatta ccagaataac caccgataat aatttttcct tttctttttc caaatctttt
    10201 catttcttct gcatttttta ctaaaacatc gatatgataa gttccatctt tttctataat
    10261 tatagcagaa ccattatcat ttactttata gactccatca cgtccagaaa ctcctgttac
    10321 aatacttact tcatcaccat atttaaataa tgggtgtttt gataggaact cttttgtata
    10381 ataagatttt tcataagctc caaccatagg tgcagcacat gtctttttag aaggattaag
    10441 ctttttctct ctacagtctg tatctccacc ttctatacca ggtccaggat aatatgctgt
    10501 aaatatagca gaaaattctg tccctcctac ataatcttct cctcctaaat tactttcttc
    10561 cttaggttca tcctgtcctg ctgacttttc atccataaga ttttgaaaat taagttcaag
    10621 ctcgatttga tactctccat tttgccaagt atgtttgtct gtatctatat aaaatagtcc
    10681 tacaagcttt gtataagaat ctttaacttt tactcctcta ccagttatac aacttacatc
    10741 tccataacct ttaagagaac agcttttttc tatcccatta aactcgctat caatatctac
    10801 atcttgattt tcttgttgct gaattacttt ttgcattatt acatttactt ccttaaaaat
    10861 ttcattatct attttttcgc taatcttact tccatactgg tctaccacta ttactttatt
    10921 ttttacattt tccatactct ccgaaaaggt ggtatttata atattaaatc cctcttcaaa
    10981 cataacactt aatgtaacag ttcctttttc aataacatta aacttatcta aattagcctc
    11041 tatcatatac tttttctttg tctttttact tgcctctgta tatgcactca ttattgtgtc
    11101 ataaccattt actcctataa acatctttgt atacttgaca ttggtctttg ctattgttcc
    11161 aactgaaagc ctattttcag caaatacttg ctttgctatg tcttcaacta acttatcttt
    11221 aaaattgtat gacacttcac tttgtgtaag taaaaatccc atatctttag atacaaaact
    11281 aatttcatta ctgcttgaat ctttagacct attaattatc attcctctaa agagttcttt
    11341 ctcatctaca tagaaacaga ctgtactagc tattggtata tctatctgtt ggaaatttac
    11401 atcaaatgat gattgtatta ttgaaaactc tagtgtcctt gatggagatt tataatcacc
    11461 tgaccatgat accttgtcta ctatgtcagt tatgtcatat atacttccgt tttttatgtg
    11521 tacccaaatc ttaataataa aaaatcacct gccttttcat ttaattctat aagcagttat
    11581 ttataactta ttatctataa tagttattgt tttggagtta cttttggtat ctttattctt
    11641 ttatactctt ttaaatctaa tgtaaaatat acatctcgag tcccatctct ttcttcataa
    11701 ctaaaccctt caattatgac ttccatattt atatttgttt ccgtaattgt aaatcttaat
    11761 ataaaacctt ccttcatcca tttttctatc ttatttacac aatcatatgg tgatggaaaa
    11821 cctgtatagt cacagaaagt gtagtcttta tttggaaaaa aacttgatat tgaaatgctt
    11881 ttaagagcta caccaccaaa agttgctatt tcacctattt tgagtatact agaagagttt
    11941 acagcagctt ttccattaat actaaaggaa gatggaaata ctggaaatct aaatctatct
    12001 tctgcttgtc taagccacat ttccactata atacacctcc caattgtgcc atttttatac
    12061 ttgaaactaa ttttgatgta actttttcta tatcagcttc ttctctaatt actattgtat
    12121 cagctaactt ggctatactt atatttccac cattttttcc acttctatat tgatttgctt
    12181 cttgtttagt taaaaccatt tcaccctcat gaagccttgt tgggtaatca ttataaggaa
    12241 catagtttaa tccaaaggcg ttacttccag atttgcttgt acctgcattc caatcatcaa
    12301 ttatagatct aactgtctca ataattggat ttttattaat tttttcaact aaattttgcc
    12361 accatttaca aacatctttt ataatattgc atatatcatt aaatagatta aaaagacctc
    12421 ctaaaactgg ttctacaata atccaagcat cctctaaaag tggtcctatt gttgcccaaa
    12481 ctgtctccca gataacacct agtgtctcta taaagttatt tatttgaggg gcatattcac
    12541 ctaaaaaagt aaaaattctt gtgataatag gactaacagc ttcaactaca ggtccgatta
    12601 gttggaatgc tgtagttact attgtaccaa atatacttat taatccttgt gcaattggtg
    12661 atttattgtt tatactatct agtaaattat ttactaagtt tactactgga agtaatgaat
    12721 ctattaatgc tgtaaaatcg atattttgta atagtgtact tcctgcgtta acaattgctt
    12781 ttggtgcatt ccctatcatt gttgctgcat gattttcaaa acgttcttgc ttattttcaa
    12841 ccatcatttt acgcttttca gcctcattag aaattcgata ttgagtagaa tcagaaaact
    12901 cttttccatt ttttacactt ctagattgca tctggtcata tttgttgcca ataattatct
    12961 taccattcat tacattgcct attttaccag ccattctttc agataatcca gctaaattgc
    13021 ctaatccact ttctataaaa gtttttattt tctttagtcc ttgacctaaa atatcatcaa
    13081 aaggtttaac aatactcttc aaaccattgt taatgcttgt ttgagctcct gatattatac
    13141 ctggtattgt attactcatt tctttagcta accccatctg ctttacactt ataccttcca
    13201 atgcttcatc aaaagtttta tagttttctc ctaccagagt attaagagat tccatatttc
    13261 cttttgctgc acttaagaaa gctgctgata catcttgttc actacttgta ttcatattta
    13321 aagctctaac atccaccata gcctgagtta tatctttttc ttttttacca ccagtaatat
    13381 tactaatcat tttagcttta gttcccattg caacaacatc ttcagggtca aaaggcgtat
    13441 catttgccat tcttactaaa ctgccataat aatcatcaac ttctctttga ttaacttttc
    13501 cagtatttct tttcattaca ctttctagaa caactttctg attttgttcc tgaccaagta
    13561 aatcaacacc ctttttcaca gcgacaccta tagtagcacc atttgtcaaa tcagatatct
    13621 tcccttttat cgaaccaagt attccacttg ccatatcctt tacagctatt gcaccactcc
    13681 ataccttacc tgcaaataat ctcaatttgg gaaatacttt acttataatt ccacttgcca
    13741 tatcttttat agctattaca ccactccaaa aagtattttt aaacaacatt aacttaggca
    13801 aaattctacc catagcacta ctcaccatat ctttaagatt aatacttcct tgataaggac
    13861 gtttctctac ttgttttaat tttcccaaga tactacttat aactctctta gtcatatctt
    13921 ttgcttttat tacaatcttc caaaccttgc caccaatctt ttttaaattt ccaagtaatt
    13981 tatttttagt tttttctatt tcatcacttt tagctttaaa tattaatata atcataatat
    14041 ttgaattaaa ctcatttttc ttttgaatca acttattaaa ataatcaata atcatagcta
    14101 tagttattgc tagtctcata atacttccat ttaaacttcc aatcactcct gaaacagcat
    14161 ttatgctaga acttaatttt gtcatagttg aacttacact actagcagta gtttgaactt
    14221 ttcttaacga agaatttaaa ctatttaccc tactacttgc acttccaata gaatttttta
    14281 tgctactagc cattacatta gtggaaagtg aaacagccac agttgctctc ctaaaattat
    14341 cagttactga tataatttga ttcatttttg gtgtgtaatt atctgtaaga tacatattta
    14401 taccttcttc tctcacattt cccattacat accacctcct gtcaaaggac acattccata
    14461 tccatgtttc attttttcaa cttcttcctc aatttctaag gcgaaaaaag cttcaataag
    14521 ctttaattcg cctttatcca ttgcataaaa aagggacggt cttatacctt ttttcttcca
    14581 ataatagtac attattgtag tcctggtatc cgtccctatt agttttttac ctcttctatc
    14641 actgcatttt taccatagcc cataacatct tgaagtattc tatataagtt ggtaatttca
    14701 cctggtaata ataattttct tgctaattct tttggtgttg gagccttaaa tttatccatt
    14761 agctccttat ttttaaataa aagctttcca tctaaatcac aaacaccttc tataattgta
    14821 agcatctgca tttccggcaa gtctatatcc gttttcatat tttcatcaat tttaagacaa
    14881 gtattttgta tttcttcatc tttacttatt gttaaagctc tacacattac tttaaattcc
    14941 tgcccaaata tagttgataa tctcttaagt tcaactatag tactcggtct ttctaagttt
    15001 tctgtatctg aatttaaaag taaatctact atgtttatat tttctgataa tccattttca
    15061 ctcattatta tttgctcctt tatcaatcaa attcaatcaa ctaacttttt tacttattaa
    15121 ctatctgaat ttattaagta tatatttact aaatatcagt ataaaaccaa atataaaact
    15181 aaattatatc aagaaaatca tactcagtaa atgtgaaagg tgcttctatc tctcctttta
    15241 cgccaacctc ccaatcagct aaagttaaat catcaaatgc tatattcttt actgctattc
    15301 tttctgcccc ataagaatct gggtcattta attttgatat agcaacaaat ctaggttcag
    15361 aacctctctt tatcttttct ccaattaact tgtgcattct tgaactaaca tggtatagag
    15421 ttattgaacc ttttcctttg tatcccatat attttgtatc agtacccatt tgacctgcta
    15481 ttataatatc ctcttttgta aattccatct ttgcttgaaa tttctttact tctgctactt
    15541 tgtttccatc aagccaaagt tctccccaag taccactcat tacatttcta gcttccatat
    15601 ttgccatatt aataatcctc ctatatttct attgataaat ctatatcttc catagcatca
    15661 agtactttta tttttgcttt taaaaatact ttagaacctg tgttagcttc ttttatttct
    15721 tgtaatgtca tataagataa atctactcct tttgatttta aatacgattt ttgtgcttca
    15781 aaatctattt caacagtaga atcagattct ataagtgcag atttttctaa ttcttctaaa
    15841 taacttttta tagctactat caataaacat ttgttgtcat aactgttagt aacctttcct
    15901 atatagtcat ctattatcac ctttcttatg tcactatgta taatatctaa agtgtcaact
    15961 atttttattt tctggaacat ttctcctttt tcttctgtta attcagttaa agaatttact
    16021 cctctagcaa ttcttatagc tcctgcttcc ttaataagta taagctctcc tttattaacc
    16081 cttgattctg catcaacttt agtcatctta ggtatatcga ctacatcaat aagcttagta
    16141 taagttactg attgacttaa aggtgtacct gctataagcc cagccaccct acttgtaaac
    16201 tcatcaacac tgtatttctt ttctccaact aacacatctt ctgtagtaaa attaattatc
    16261 ccttcatgat ttcctacaac ttttcctaat acagctttaa ccttaacctt atctatatct
    16321 ctaagtttaa ttatccaatt ttttatagca gtcttatcag cttctactgc ttttggcata
    16381 catagataat taaattcctt agtctctaaa aaatctaatg cagtttgaat atctgagtct
    16441 ccttctatta cataaactaa taatttattt ggagtgttaa catttcccat caaagctaaa
    16501 tttatatatt ctttattttc agcagataaa tcaactggta tatcctcttt ttcatgtatt
    16561 tcgttaagac ctaatgcctt agcgtcttta agcaccattg caattattcc tctagctgaa
    16621 cgttctttaa cagttgtagc tagctcctta aatgatatgt tgatacttgg taatcctata
    16681 gccatactat cactctccta aatttaaatt gatttcttcc atattttcat atactacatc
    16741 ttcttcatat acactatcca gatagtttag tgtaaccgaa aaattcagtg cataaattga
    16801 ctcttctttt ttaatacttt gttctagctt tgttatattt aattttctgt ctgttacctt
    16861 tatatttctg ttgaataact tatttaattc atctgaaatt tcatacattc taatactatt
    16921 tgactgtggt tttggtaagt attttataga aataacaata tctttattat tcgtatttaa
    16981 cattataggt gtacaaataa ctgatagtat acttacaaaa aaacaagacc cttcagagcc
    17041 ttgcacactt tcatcatcaa taaaaatact ttcagtaaag ttttttttga gaatctcaat
    17101 aattgtttct aatatttcct tgtatttaag caaataatca ccacactatc actaaatatt
    17161 cttgcttaat ctgataatct tactataaat ggtataagcc atttttaaat atacatctca
    17221 tttattcctg tcaagtatct tctatatttc taggtaatat tattcttttc aatttatcac
    17281 ctccttcaat atggatttct ttcacaataa cattatctca catattttat tgcctattgt
    17341 tgcaatggtg tatataaagt gttgtaaaat tgtcctattt tttataatat ctcactcagt
    17401 ttaaatatca tctttttctt taaattgtag caagatgttc tatctaagtg tagtttcata
    17461 gaaatatatg tcatattgtt ttttgtctta ctattataaa aaagattaaa aaattccatt
    17521 tctatatcat ttagacatgt tagagcattt tctatcttct ctttttctat ttctatatct
    17581 tctttttcca tttttaattt tcttaatctt tcctctcttt ttaatacctc attttccaca
    17641 gaagaactta tgttatatgt gttactagtc ttttctgtat ataccattgc tccacatcca
    17701 gagtattcat tctcacatct ttttatgtcc aagttaatat tctttatctt tatatccaaa
    17761 tacttataat tatgtaatct tttatctgtt tctttaaata atatatcttt tttgttttcc
    17821 atagtacttc tcctttttta tataattatt attagcttca aatttacaat aatctcttta
    17881 gtgcacaatt ttttactaat ctcacagctt tcaagaacat attgttctat aactgtataa
    17941 aaaaaacttc ttctatattt tgttctaaaa tactagaaat cttcttagcc ataagaagtc
    18001 caggattttt tatacctctt tctatttgag atatatagtc atttgtaatt ccagcctttc
    18061 tggcaagttg aatttgtgtc atacctttgc attttctata atatttcaag ttatttttaa
    18121 acattgtttt atacctccat tactattaag aatattttgt tcttccatat ttagattata
    18181 tagaattatt tgttctttat caatagattt tagaaaaaaa tattctttta tattttatta
    18241 gttttatttt ttagaacaat ttgttataat ctatacataa ggtggtgtta taatgtttgc
    18301 caaaagatta agagaactta gaaaggaatt tggattgact caaagagaac ttggagaaaa
    18361 agtaggcgtt tcccaaagag tcttagggta ctatgaaaca gaaaacagat ttcctgatga
    18421 acacatttta aataaattgg ctgatgtatt taatgtatca gttgattacc ttcttggaag
    18481 aaccttagtt aaggaaaata ttgatacagt agctgcacat agaaaaaatc cacatgaaga
    18541 gttacctgaa gaagcacaag aacaacttaa tgattatata gaatttttac taaataaata
    18601 taaaaaaaaa taaaccatat gatgagcagt ctatttttct gctcttttaa tataacttaa
    18661 aggcaaacat acattctatt ttagggggat ttttatgaat aatttggata agctttttga
    18721 attagcttct caagaagaga taataattca ttataccact tatattgcag gtgatttaga
    18781 aggactatat ataaacaaac atggtattaa aattatatca ttactcagta atttaaaaca
    18841 aaactcaaaa aaattgacat ccatcttagc tgaagaactt gggcatcatt tcactagcct
    18901 tggatactat gtatcctctt ataatgatta ttatacaaaa attattatag ataaatgcga
    18961 aaataaagcc ttaaaatggg cttgtgagtt cttaattact gaagaagata taataaacat
    19021 aattaattct ggcatcactt gcgtttatga aatggcggac atacttaatg tggatattac
    19081 tttctttcaa aaaagattag aatttttatc attaaaaaaa cagtctttac aacttggaaa
    19141 taataaatat ttaatattaa ctaatttgcc gtatttctac atatttgatc caatttcata
    19201 ataaaaatag tagtaatttt aactggctta acagtaaaat ctatatattt attttaacaa
    19261 attttggttt ctataaattc aatattaata tataataatg atatcatatt atttaaaatt
    19321 ctaaaaacaa atatatgctt ttgttaagaa aggttgtttg atgaatgtat aaagaaaagt
    19381 tgtttgataa ttattttaag ttcttagcac tattattttg gccaattatg tggtataagt
    19441 ggatagtaat ttcaaatggt actcttgaaa atatgctctt taccatatat gcaatcatcg
    19501 caattgtttt catcatttta tattcagtgt ttatgataaa atataaagac attacacaaa
    19561 tagatttctt ttacaggata agtactctac ttgcttttat atttacactt tttagctttt
    19621 taatataccc aaaaagttta tttttcttgt atttaaagat tatttttact ggaatatacc
    19681 tttattattc aatagtaaaa acccttaaat ttaaagatga tgaaggtgta gtgggtatta
    19741 tgagctcact tttattaatt gtaataactt tgttctacta aatttaattc tatttgactt
    19801 aatatggctt taaatattag acatctacat tattttctga tattatttaa gatggtagtc
    19861 ttatattaat aatgaaatac actatgaagg agttaactat tatggaaaaa aaagttttag
    19921 caacagttgg agaaaaagag ataactaata ttgatattga aaatgcatta aaatcattag
    19981 acccttatca agctatgcaa tttaaaactg aagaaggaaa aaaacactta ttaaatgatt
    20041 tagtaaatca agaattattc ttcttagatg ctaaagaaga aaaattagat gaagaagaaa
    20101 tatttaaatt agaaatgaaa aagatagagg aaaatgtatt aaagcaattt gctataaata
    20161 aagttttatc tagtgttaat gtaacagaag atgaaaaagt aaaattcttt gaagctaata
    20221 agagctcttt ctctaaacca gaaagtgcaa ctgctaaaca tatattagta gatagtgatg
    20281 aaaaagctaa agaaatactt gctcaaataa aatcagaaga aatatctttt gaagacgcag
    20341 ctcttaaaca ttcttcttgc ccatctaaag atatgggtgg tgatttagga acattcggta
    20401 gaggacaaat ggttcctgaa tttgaagaag ctgtattctc tatggctaaa ggtgaagtaa
    20461 gtgagccagt taaaactcaa tttggatacc atataataaa attagaaaac cttcaagaaa
    20521 gtacagagtc tacatttgat gaagttaagg ctgaagttga aaaaagttta ttatatcaaa
    20581 aacaaaatga agtttatgga aataagataa atgctcttaa tgctaagtac ggaaaccttg
    20641 taaaatacaa cgattaatca taaggtcata tataaaaaag ctgtcttttc acaatattta
    20701 ttagaaaaga cagcttttta tattaaagtt taaatatcca gtttcatatt atcattcttt
    20761 tattgtaaag aactcagctt aaaagtttaa ataagatttt taattatcat tagctaaatt
    20821 tttatatcca cgtattatct catagcagta acctattaca tctcttcttt ttattatccc
    20881 aataaatgtg tcttggtcat ctataacagg aatgaaattt tgatttaaag attttataac
    20941 taaatcttca atatctgcgt tgatggaaac aggagaatta tccatttttc tatttatatc
    21001 tgtcactgga acatcttcca atccctttaa gtccagattt aaatcgtttt ttaatgtcca
    21061 taaaaaatca ccttctgtta tagttccaac atatttacca tccttactta ttattggaat
    21121 tgcagagtat ttgtgatatt ccattttttc tagggcttgc cttatagtgt agtcttcata
    21181 tatatatgct acttcacttt ttggtgttag aaagaataat atgttcatta cactacccac
    21241 cgttactgaa aacttataaa tattattcaa tatttataat gttacttttt tgcagtaact
    21301 ttctcctttc ttattttcta agtattctta atactatacg ctgtaacaag cgtttttcct
    21361 ttgctcatct taatcttatt atgctttatt aatattttta tatggtctat cagtatcact
    21421 tattctcttt ttagaaaatt cactaaaaat taattgactt aaacttagcc ataataattt
    21481 tactctacac tattaatttt aacatattta tctcattatt aatgatttta ttttaataat
    21541 tttaatttat tttgatatta attttattta caaaaactga aaaaaccgct aaatgattag
    21601 cggcttatct ctcatattac tagaacacta atatatatga atttttaatt ataatctaac
    21661 tacgttagca gcttgaggac ctttagctcc atctactact tcgaactcaa cgctttgtcc
    21721 ttcttctaat gttttgtatc catctccagt tatagctgag aaatgtacga aaacatctcc
    21781 ttcaccttct acagatatga atccaaatcc tttttcgttg ttaaaccatt taactacacc
    21841 agttttcatt aataaatcct cctaaaaaaa cacaaatatt tagctactct tttctgtaac
    21901 cttattacta ctctatcata tatagctatg tttgtcaatt attattattt ttatacatta
    21961 ataatcaatt tttttacttt ataattttaa atcgttatta tcttttacac tttatttttc
    22021 tttataattt tttaataatt tatttttatt atatggaatc ttatggactt atcactccag
    22081 cctaaatccc atggcaccaa cattctatca gtattatggc aagttaccaa aggatatcct
    22141 cttgagtcta atcctgtcac tgtcgaaaca tgtgttattc tgcctttctt ctcataacca
    22201 acaaaatccc ctggacgtaa attataagct tctttgtata cttcttcata agaaccttta
    22261 gcaattaatg aacctcttcc actatacagt gcataatttt taaaagcttg tgcatttacc
    22321 catgcctttg tacctgctct ttcgctataa ttccaaattg agttcttttt gaaccttcca
    22381 ctttcaaaca ttatctgtga tgcaaagttt gcacaatctc caccttctgg attataatct
    22441 ctatagtttg gattaaattt cataccatgt tcttcatcag ctgcggctcc acagtattta
    22501 tgtgcataat ctatagcttt tttttgttca tctgtaagct gtaactttac accatcttgt
    22561 tggccaatat aatttcttat atcatcagat tttatatttt ctaaatttaa agagtctgca
    22621 aaagggtctg tataccactc tttagttatg acatattgat tatctataat ttttacattt
    22681 agataatgat atgtacctat tctaaaggca ttttctttgt ccttttgatt ttcatatgaa
    22741 tacttatatt cagtcgatac tgaacagatt ataccataaa ggtctttttc tttttccttt
    22801 atttttttaa tcttaaccct tgtctttatg tctttaaatt ttacaccttg cttactagac
    22861 cagttttcta aatacttcat cttttttact tcattttcat aagcccacag accaaatttt
    22921 ttatccgttt cataaagacc ttttaaaatg ttttcatttt tctctaatat tgccttattt
    22981 ctgtagtcaa ataagttttc aagcaatagt ttatattggt cttttacatc atcaccttca
    23041 acatttgcag attccatgac tttttcttcc atatttttca tacttaaatc attgattaaa
    23101 aatacacctg ctgtagtagc taaagacaaa aatacaaatg ccattatacc tttgatgtac
    23161 ttattcttat ttttcagtat tttcaaagct acacctccca aatagtacac cttacaaatc
    23221 aaattacaaa gaatattctg tcacatacag taattttcat atttaataat tctctatttt
    23281 attatgtcca atattaaaaa ttaattgtat caatacactg ttttttttac aattttttat
    23341 tttataatct acttttttct tttttttata gtaatatata tgtatataaa tattttggta
    23401 tcattataat taatacatac taatggaggg ttttttatgc aaaaaaaagt cgcggcaatt
    23461 aatgacttat ctggaatagg aaaatgctca ttatcggttg ctatacctat actttcagca
    23521 ttaaaggttc aatgttgtcc atttccaacg gccatacttt caagtcaaac tggttatcct
    23581 gaatttactt ttttagactt tactgatgaa atggtaaaat attcaaatgt ttggaaaaat
    23641 ttaaaagtca attttgatag tatatatagt ggttttttgg gttcaaagca tcaaattgag
    23701 atagtggcta attttataaa cgattatccc aatgctttta tcgtagttga ccctgtgatg
    23761 ggtgataatg gagttatgta tccaatattc acagaagaaa tgagacaaga aattaaagaa
    23821 ttagttaaac attcagattt gactactcct aacttaacag aagcttgttt cttaactgga
    23881 aatgattata ctaaaagtga ttataataga gatgaactta tatatattgc aaaatctgta
    23941 tctgatttag gacctagtaa agtcgttatt actggaatat tagaagacga taatatacta
    24001 aatttggctt atgatagaga taatgaccat gtattcttta catcagtcaa atacaataat
    24061 tgttcttata gtggaactgg agatatattc acatctatac tttgtggcat gttagtaaat
    24121 aaacatgatt tgggagtagc ggttaatact gcaacagatt ttatatataa aactataaat
    24181 tacacatctc aatttgatac cgatagaaat gatggagtta tgtttgaaaa ctttttatcg
    24241 gatttaacta atatataaaa tcaaaaaaac tatccatttt ataaatacct ataaatcata
    24301 aaatttaggt atttacaaaa tggatattat ttattaaact attggtattt caattgttac
    24361 catagcacca ccagttttca cagaattttc aatatataaa gtcccattat gtttttgtac
    24421 aatattattt acaatataaa gtcccattcc ataatgagat ttagaatttc tactcaaatc
    24481 tcctgtataa aactgttctg tagcttttga caaatctgct ttagaaaaac cactcccaca
    24541 atctgtaagt acaaatttta agcatttatt acttactgat acacttatgt atagtttact
    24601 ctggcttggt gaataatcta tagcatttga aattatattc ataatagctc tgaataacag
    24661 ttctctatct atagttatat ttttaggcaa attatcttgt ttataaaact ccacatctaa
    24721 gtttttagta ctagctaatg cttctatttg cactaagata tcttttataa attcactcat
    24781 attaattact tctttattta atatatagcc attctctgtt tttgatatat ctattaaagt
    24841 attaatatat ttttctattt gagtgacacc ctttgagata tattctatgt attctaataa
    24901 ctctgggttt tcatttattt caattaataa gtcagtattt ccatgtataa ttgttaatgg
    24961 tgtttttata tcatgtgcca gtgcagatat ctgttccttt ctattttctt ccaacatcca
    25021 ttgtttttct aaagaatctt ttaacgccaa cttcatttta tccatagaaa aaagaacatt
    25081 attaatttca taaattttac tagattctac aacaaaatct aaatcctgtt gttctatttt
    25141 ttctgtagta ttttttaaaa gctccatttc aatattaaat ttcttgccaa atacttttga
    25201 taatataaca atttcaataa aaaatattat actaaaaata atcataccca acgtctctgg
    25261 ctttggtaaa tatgctctta aaactggaga actatattct gatactattt tgtattttat
    25321 tataaaaact tcatcttttt taaaaaactt tatataactt tccgaatatg catgcctttc
    25381 ttcaatatct ctcattaaat tccatacttc ttttgattct tttttattaa aagtacctga
    25441 aattaaattc ccatctaaag tataaacccc atactcgtat atatttggaa ttatatcttc
    25501 agttactttt tcacttgaaa taatcttttc tttatatcta ttaatttgtt tttctgcata
    25561 attagctggt aatattacac ctgatgataa tagccaagaa aacaataata tcaaaataag
    25621 tacaattgaa attgtcataa taaagaataa agataaatat tttataaaaa tagttcttaa
    25681 cttttcaact tttttgttgt tagatttttt acctccaagc atttgtacca tgtcctccaa
    25741 tagtttaatt aattcataat taatacctaa ccagtttttt atttgattat tttatatctt
    25801 tttattgcta agttatattt taacataata atataaataa acagtcaatt tttattctat
    25861 tcttctgatt ttttaccttc ccatttagaa aaccaaatac ataaaattac aaatgctaaa
    25921 attgttacaa aaccacacat taaacctgtt gattgatatg cttttattga ttcaaccttt
    25981 atatattcaa ccgttttaga acttgaaaaa gacgaccaaa tagttataaa tctaacaccc
    26041 catgcacaag gaaaatactg ccaaatacca gaacccaatc cagttagtaa taatgctgaa
    26101 agtaacattt caactatacc aactccaatt gatgctcctt ttccgaacct taaacttaag
    26161 aataaatgta atatatattc gaataaacta cacccaatta aaattaggat tgaaatcata
    26221 taaaagttgg atgtaaagta agcctcagat ataccaaatt taaaacctag tactgacact
    26281 aatgttgcta caaaaccaag acatactaag attacatatt tacttataag agtaagatat
    26341 tttggattag atgttgttaa catatgttta taatttcctg cataatattc ttgttcaacg
    26401 aacattgaac acacaacacc tgacaataaa ggaaaagcta ttgctataac ttgtaaatat
    26461 gctactatct ttagtatatt atcagctcct gaaaccttat aataccataa aaacaatccc
    26521 atccccatta aagcaatgca aatgtgcatt agtattataa aatttcgctt taattttatt
    26581 aaatcagaac aaaatattct tattagctgt ctcatattac ttcacctctc tactattgaa
    26641 ccaaaccctt gtagctaaag caaataatac aaataaaaca atagatatta atactcctgg
    26701 taaaataaca gcattattta aaagttcact tccctctggt acaggaagcc catttggcag
    26761 aatatgcaat acaacagcca ttaatcttga aggaatcgaa tatggaaata tataccacat
    26821 ttctgtagtt gagaaaacag caactccaac aatagtaaag aacatatttg ttagaactgt
    26881 aacaaaaata ccaaattttg aagctaaaaa catacaaaga ggaatctgcc acataaatag
    26941 taagataatt aatatagttg caataatatt ttgacttatt gaaatagttt ctttatagat
    27001 tatcccaaca aaaactacac ctattgcaga aataattgat gctataaaac aacaaataat
    27061 acagtaaatc atttttccag tccaaacgtc tttcatttca attggcaatg gtaaaatacc
    27121 acgatattta cttttttcat cttttaatac tattaaagaa catattaatg ttaatgctcc
    27181 tggaagaaat gtcgtatacc accagttgaa agcagcattt tgccctccac ctagtccaca
    27241 aaaaagtata caaaacactg gaactatcca tatcaacttc attccaaaag ttcctttcat
    27301 cttttgaaat tccactctaa taaatctttc catgttactc ccctctcttt ctattttcag
    27361 ctacaacttt cataaaaagt tcttctaagt tttgttcttt tgatacttct ccttgataac
    27421 ccattactcc attagaaatt attccgattt catctacaac ttgtttaact tcagacaaga
    27481 tatggcttga taaaataact gtaattcctt gttttggaaa agaacgaatc aattctctaa
    27541 gttcttcaat accaataggg tcaagaccat ttgtaggctc atctaagatt aaaagttttg
    27601 ggtgatttaa taatgcaata gcaataccaa gacgttgctt catgcctaga gagaattgac
    27661 ctgaacgctt tttaaaagta tctcttaaat cgacaatctc taaaacttct tcaatgcgag
    27721 agtcaggaag accaagtaaa gtagtatgga cttttaaatt ttctcgtgct gttaaattct
    27781 catatatagc aggagactca ataagagaac caatatttga taaatctttt ctactccatc
    27841 tatgtccctc aaacattatt tttcctgaag ttggatttaa aatcccagtt atcattttta
    27901 ataaagtaga tttcccagct ccatttggtc ctaataatcc atatatagaa ttaggtttaa
    27961 ttgctagtga aatgttatca actgccatct gctccttaaa ttttttagaa actcctttta
    28021 cctgcaaaat ataattactc ataattttca tcctttcttt tgtcaaactt atttacaaga
    28081 atcattctaa atcataaata taaggatttt ataagaatta aaaaacaaaa aaagatgtgt
    28141 taaaaaacac atctttttta tcttaattct attaattttc cattgtagta tatctctact
    28201 tcttcaatcc aaatgctatt atcttcttct tggatgacat ttttaataat attatttata
    28261 gtatatatta tattttgttc atttaaataa tcatctttcc aagaaactct acttttttta
    28321 tctggctcta tcaagtttat tttaacaaga ttatcttttt ccttaacttc tacttccatg
    28381 tctaaaccat taaaatactc atttgataat gtatctaaga ttaatcgcaa tttatcctta
    28441 agtgaacttt cttcatcaat ctgtaagtac tttactattt tctcatcctt tgagattatc
    28501 tctataggta ttttagtagt tttcttagaa ttaatcattt tactagttgt ttcttctttt
    28561 gcatcttcat ccttctttac aactgctgat tctttagaat caactagctc aactgcactg
    28621 atgctataat ccatacatgc tgtactcaat aaagtagtag caattattaa aaaacttact
    28681 gctattttat tcataagtat ccctctccta attctctttc gatacttatt ataccatagt
    28741 atagattata tttttatact tttaaatcaa tttataaatt attatagtaa attatttaca
    28801 attctaagct atttgaattt atttttatag tatattcttt ctctaaattc gacagatttt
    28861 tattcaaaag cccttttatt tccctcgtat ttctatactt gttgctatgt tcttcactat
    28921 gttcataatc atatgaatca ttgtcatatt cttcatcatt attaaattct tcgattactt
    28981 cttttgactt tatatcacaa ttaaatttat acttatttac tggtatatca agtcttacct
    29041 tagaatttgg aacttctatg tctaagaact tagcaattgg catattagag tacaagtaaa
    29101 attcactatc ataatttgaa tttctaccta ttttctcttt tatatttaca ttttcaggta
    29161 tatactcttc tatattatcg ataaatacat cttcattctc tgaagatata tccaatgact
    29221 ctgattctat gtttacattt ttaacaccta aaattgattt tgttgacaag tgaagattgc
    29281 tattagatat taagtttaag ttttctaatc tatttacttt gttctctgtt atagctctgc
    29341 taaaacctcc agaagaagtt ttatatgtta tattatttga agctacatta tcgtatacaa
    29401 ataagtatga acttatactt gatgcattta tatctacatt atttggtaca tatattataa
    29461 tttctctata atcatgtgaa aacatactat ttatagatgt atttaataat tggtcgaaat
    29521 ttttaatctt tttaatttta ttttcgtatt ttctttcacc ttttacaact aaagtcttat
    29581 cttttaaagt tacattgtat ttgtaaaact cattatttcc tcttgttgtc acacgcacat
    29641 cttcacttga acttttcttt attgtaacgt ttatattgtc aacagatata tctaacttgt
    29701 caatatttac tttttcttga taaagtttat tttctttttt aaattcagct tccttttcta
    29761 aaccaaagtt tattaagctt ggcataacta aaatccctga ccatgttgtc cctattatac
    29821 ctattaccaa taaaaccgca gcaaatatag caagtttttt attcatgttt atcttctcct
    29881 ttcttattgt ctatatcttc aaggtcctta tatatcgcct caaaatttat actatcatca
    29941 ttatattcat cagtttcttt tatactttct tcttctctat tgtagtaatc attattatat
    30001 gaatcttcat taatgtcttt attcaaatct tcatcattta aagtagcttt ttttcttttt
    30061 tccatctttt catttgcctt tatataaata ttttttctct ttatccaatg tatataatta
    30121 tttagtaatt gtttacctag ttttatggca aatagtaaaa tttgccatgc aagtatctca
    30181 atccctacaa atgctatggt tgcgaaaata caaaacatca taacatctgg aaccattgct
    30241 ataaatttaa tcacaaatgg aattaatata acaaaagcaa ttaatgaaca tactaatgca
    30301 atagtaacag atacaatagc acatacaaac gcaaatgctg gaacaattag aaataaagat
    30361 aacactacta ataatccctt tataacttgt ctagataatc cccctacacc ttcttctata
    30421 cttggtgtaa gtttatcatt tatattttct tttgatttat tgtacttttc cttagcttca
    30481 tctttcaaga tagagtaatc cttttttata tttgatttaa acctactaaa tttaccattt
    30541 aatatatctg tattcattat ttttccatct atattttctt ttgatttact atacttttct
    30601 tttgcttcat cttttaagat agagtaacct tttttcatat ctgatttaaa tttactaaac
    30661 ttatcattca aaacatctgt atttattatt ttttcctctt caatattttt ttctttagtc
    30721 tgcaagataa gttctttggc tatagatttt ggtgaaccaa gtgatgctat tatctccata
    30781 tcactctttc cctctataag accatctacg aaatactctt cataatctct caatatatca
    30841 ttaacttcgc tctcagaaaa atcttttttt agataatcct ttaaaatttc aagaaactca
    30901 gttttattca agttcattac ctccctcatc ttcacttatt aaaatctcaa cagcctttac
    30961 aaattctctc cactcttcca tcagattgga taaattctct tttcctagtg aagttattct
    31021 atagtatttt ctagcaggac cttcattaga ttctagtata tatgtttcga aatacaaatc
    31081 ttttgtaagt cttcttagaa taggatatat tgttccttca tttacatcta ttactttaga
    31141 gatgttatgt actatctcat atccatacat atctttctta gatatgagag caagtacaca
    31201 aatttctaaa acaccctttc taaattgtgt gttcatatac tccatccttt ctttaaatac
    31261 acagtattgt gttttacaag gtactatgtt aaaacttctt atatcctcat aataacacag
    31321 tactatgttc tgcacaatag ttatttttaa atattcttgt tgattaaatc cttctataaa
    31381 tgtataatac tctaatcaga tatattttat gaaagtggtg ataaattgac taaaatgaaa
    31441 gatgataaaa caatattaat cttaactgct caatttggtg ctggacatat aagtgctgca
    31501 aaagcagtca aagaatgtat tattgaaaaa tatagtaact ataatgttgt tatacaaaac
    31561 tttataaatg caagtattcc catgatgaat aaacctatgg taaaacttta tgaaaacaat
    31621 acaaaatata ctccgggatt atataattac tattattact ttaagaaatc gtttgattca
    31681 agacatgatt tatctcataa attatataca cctaaacttt ctgagtatat tgctgatata
    31741 aatccagatt tgattatctc tacatttcca ctagcggctg cttgtgtaaa taactttaag
    31801 ataaaaaatc ctgatataaa tatacctaca cttacagtta taacagatgt tgtagatagt
    31861 atggaatggg tttttgaaaa tacagatttg tattttgttc cttctcctga aataaaaaat
    31921 agattttttc agaaaggaat aaatccagac tccataaagg ttactggagt tcctgttgat
    31981 aagagatttc aaattgaaag taaagaacta tgttgtgata aatatagact attacttcta
    32041 ggtggaggaa gaggtctatt tgatatagat gaggatttta tgcattggat agatgaattt
    32101 attgaagaac atagtaattc tatagaaatt acaatagtta ctggtaaaaa caaaaaatta
    32161 tatgacaatc taactcataa aaaacctcta aaaaatatta aagtacttgg atttgtaaac
    32221 gacatgtata atctaataag agagtgtgac ctaatgctaa ctaagcctgg aggagcaact
    32281 atatttgaag ctattcaatc acaaactcca gtactcgtaa aaatgccaaa agttggacaa
    32341 gaaattgaaa atgctaaatt tataatagat aagggtcttg gcatgattta tagtgatgac
    32401 ctagacctta aaaacatatt ttataaatta gtatcaaatg aatttgattc tataataaac
    32461 tttatgaaaa aaaatttaga ggaatttaaa acagtcattc atccagagaa aattgctgat
    32521 tatatatcag aattaataga taatcattac agttaaattg tactattaaa aaatttcaaa
    32581 cataaaatta aaggcaatca agtatgcttt tacacttgag aatttactca tagtgtttaa
    32641 taatactcga ttgcctttat catatcaaaa tcaaattgtt atattagtat atatttttct
    32701 tgtactgcat atacagttgt tgtatttctt ccatacttct ctttaattct atttgtaaat
    32761 ctgaagcagt ctggtaatct ttattttcta tagcttctct cactctagtg aatagtgatt
    32821 ctctagttaa agtatccttc ataatattga attttaaatc cgatattgag ttccaaacta
    32881 cttcatcaag cgcatccata ttttcttttg tgtgtatctt gacaatactt cttatttttt
    32941 gaatcccagc ctctattatt ctagttttta atttcttttg ccacttatct atagctccaa
    33001 tcttaaatga ttctattata ctatcagtaa atacatcacc ttgttttaaa gattttaatt
    33061 ttgaagcata tatttctaag tttttcatat tttcataaac tgtagctggt ggtattccaa
    33121 atcttgcatt tctttcttcc aaactataat gttcaaatac atcattttca tctctatatg
    33181 ctctatcttt ttctaaatag aaactttctt cacctacatt tttagataac tctttttcaa
    33241 gttcttttgt actaagacca ctcttagctg ctgcttttat accatccaac attgtttgat
    33301 aacatcctgc tattactaaa tacgtgttag atagagggct tggtgacctt aactcaaatc
    33361 ttacagtctt tggatttttc atatctctta ttaatccaac aagcactgac ctatttcttg
    33421 atggaatctc ataactatgt cctaaagatg taactatgca tacaggagct tcaaagccag
    33481 gaactaatct attaaatcca tcattagaag ctgttacaat tgggtttaat acttcataat
    33541 tgtaaagaag tcccataaga gccccatatc ccagttcact tagataatct tcttttaaat
    33601 cttttggagc aaataaattt tttatacttc catcctttaa cttagcactt actcctacat
    33661 gtgtatgtcc accacttcct gctactccat gaattggctt tgctttaaat gtaacttcta
    33721 gtccatgtga tgtaaataca tcctctataa catctcttac taataattca ttgtctgctg
    33781 cttgtaatgg tgtagaaaac ttccaagaaa cttctaattg ctccatagca tggtttgttc
    33841 ttccatctat actaatagaa ctttgtattc ctccgacctc tttatgagcc atttcagggt
    33901 tcacacctag tttttgaagt ataattaaac ttttctccaa acaagttctt ataataccat
    33961 gagttctttt ccagtattgc tcttttaggc tttgtgatac atataatttc tctaggtcag
    34021 ctttatcctc aggagtatta acccagaact caagctctgt agctgctgtt aacattattt
    34081 cctctatatc atctactgaa tctattccta tgttatttat tacatgtgga tattctctaa
    34141 atatttcata cattgacttt ttaaagtact tatctgcttt ttgaagtact cctctagagc
    34201 atactttttt attatcatgt attaaaaatg caggtatctt taatgttcca acaggtagac
    34261 ctacttcttc gtctatatat tccatattgt aatctacata ccaatgacaa cttttatctg
    34321 gcattaaatc tacttttgca ttatttaatg tagctatgtt ataaagctca acacttgaac
    34381 catcagtctg tatagctgat tccaaaaatt tatctatatc ctctaaaaat aattctacag
    34441 gaatcttttc atctgttgca tttcctccta aatcaacccc cattaaggaa acaaatctta
    34501 tatttttatt gctttttagg atttctctta attcttcatt agtgtgttta tctttctcta
    34561 ttacgtataa tagactatcc atttttccac tctcccataa tttttatgta tattttattt
    34621 tgttaaatac aagataaatt tcattttcct tacatttatg aatttttctt gcaaatcatt
    34681 cgtgttttat tcgtgttttt atttatatta ttatatatca agtacaaaaa gttcgcaata
    34741 ttttttattg gaaaaaatat tatacataca atagtttaac atttttacaa atatggggta
    34801 tactaaaata aaagtatttt tatttgaaaa tttaatttta tttataatat aatctatagt
//
LOCUS       DAEPXF010000020        33156 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain HB27
            SAMN13376389-rid12479423.denovo.021, whole genome shotgun sequence.
ACCESSION   DAEPXF010000020 DAEPXF010000000
VERSION     DAEPXF010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13376389
            Sequence Read Archive: SRR10518432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 33156)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33156)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001069624.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001069624.1
            Genome Coverage       :: 315x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/15/2021 01:54:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,165
            CDSs (total)                      :: 4,076
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 89
            rRNAs                             :: 10, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 10 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 19 of 69
            Pseudo Genes (incomplete)         :: 44 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 10 of 69
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33156
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13376389-rid12479423.denovo.021"
                     /strain="HB27"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="China: Hebei"
                     /collection_date="2017"
     gene            143..1336
                     /locus_tag="KRL67_000881"
     CDS             143..1336
                     /locus_tag="KRL67_000881"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HBH1521786.1"
                     /translation="MDTLKELFKIGSGPSSSHTMGPQRAAERFKNENPDAESFRAILY
                     GSLAATGKGHLTDYIIEKTIAPKKVEIVWEEDIIKDFHPNGMKFEALDKDKNVTAEWT
                     VYSVGGGTIAEEGQRNSKSNSIYHLDTMDEIVKWCKENNKTLVDFVLECEPKDIKDYI
                     KTIKDAMRKSIDDGLSTDEIIPGKLLLKRRASTFYNAYKKDKSFSTLVYAYALAASEQ
                     NASGNIIVTAPTCGSAGVIPGIFFAMQDFYNYDDEKIIEALLVAGIIGNIIKTNASIS
                     GAEVGCQGEVGAACSMAAAAVAYLKGGTIDHIEYAAEIALEHHLGMTCDPVYGYVQIP
                     CIERNAMAAQRAYDAANYALLTDGSHSVSLDQVVETMKETGIDMMDKYKETAKGGLAK
                     HFFSC"
     gene            1542..2660
                     /locus_tag="KRL67_000882"
     CDS             1542..2660
                     /locus_tag="KRL67_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyldiaminopimelate deacetylase"
                     /protein_id="HBH1521787.1"
                     /translation="MREASYLQEQLYKHRKSLNPIAEIGRKEYKTSKYIRDYLDKLGI
                     EYEVYLETGIVGVIEGKRPKKDIAFRADIDGLHSEEGVKHLCGHDGHATILLGLIEYL
                     NDNKEDLNDNIVFIFQPAEEGPGGAKALIDEGVLRKYNIDEIYGLHIYPELPEGYVGT
                     REGYFMAQIGDIDIEIVSKSGHGAMPQNGIDGIVIASNFVSSLQTIVSRNISPIDNAV
                     LTIGRIEGGARRNIIAENIKMEGTMRCFNPEVYEKMKLRVRELARGFGLAYNCKVNIN
                     IIDDYIAVKNDKDLYQEFVEAIGSDTVVELEPLMISEDFSYYQKEVPGLFFMLGSRNE
                     EKGFVNGLHNINFNFDEKICVNALNVYSKLLKYKEAID"
     gene            2791..3036
                     /locus_tag="KRL67_000883"
     CDS             2791..3036
                     /locus_tag="KRL67_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small, acid-soluble spore protein, alpha/beta
                     type"
                     /protein_id="HBH1521788.1"
                     /translation="MKEKIQREKKITNGKNTKILTNDDIMKYEIASELGLMDKVGERG
                     WAGLTAKEAGRIGGMLTSRKKRLKKIVEEQNKKDESI"
     gene            3023..3769
                     /locus_tag="KRL67_000884"
     CDS             3023..3769
                     /locus_tag="KRL67_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HBH1521789.1"
                     /translation="MSQYSDFAFVYDELMNEVDYNGWVKYIEDIIDSEGVKVKNILEL
                     ACGTGNLTIPLTKKNYDIAGIDISDEMLSVAREKAEKEGVELVLLQQDISELDFEISD
                     LDCVLCACDGFNYITYDDDLENVFSKTYELLKKEGIFIFDISSFYKLANILGNNMYGE
                     NREDIAYMWQNYFDEEENLIEMELTFFIKDECGKFERFEEIHQQRAYTEDEIVDMLKK
                     SGFKDIKYYGDFTFDKPSEESQRIFFVCKK"
     gene            4053..4928
                     /gene="hslO"
                     /locus_tag="KRL67_000885"
     CDS             4053..4928
                     /gene="hslO"
                     /locus_tag="KRL67_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HBH1521790.1"
                     /translation="MRDYVLRATSGNGQVRAFVATTRNTVEEARRLHETTKVATAALG
                     RTLTATSIMGLMMKNDSDKLTVIIKGGGPIGTIIATSDSKGMVKGYVGNPQVEVEDYP
                     NGKLNVAAAVGTEGVVKVIKDLGLREPYNGTYPLVSGEIAEDFTYYFAVSEQTPSVVA
                     LGVLTKEDEVEFAGGFIVQLMPDAEEETIAKLEENVAKLPSITNMLKEGKSPEDILNI
                     VLDGLEPKILDTCEVGFMCECSKERVKTALVAIGKKSLAQIIEEDKKAEVGCQFCNKK
                     YMYSEEELLEILKEM"
     gene            5319..6119
                     /locus_tag="KRL67_000886"
     CDS             5319..6119
                     /locus_tag="KRL67_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HBH1521791.1"
                     /translation="MESKTKYIGISILILLMIFLIAKNFNGKLEIPIPESNSRKFKSA
                     AVFYPQHQDDEVLWGGSAIVDAIKQCGVDNVYVVLVSDGSGVNVFKANTKFRNLTRKQ
                     KEELRNNEFKSALRELGVKPQNVIILADIDKKEGTHYELMEKTILDFEYKFKGNVTHI
                     AHHYKYDDHIMHRKNGEVLKKLKRERKIKDDLYFIKPKYVKYIPRNERAIYKALDFDD
                     YNKVKSACYEYKVVNSKRKMYGIGYISAHSYFEHLLNDPELTSVLSED"
     gene            6645..8885
                     /locus_tag="KRL67_000887"
     CDS             6645..8885
                     /locus_tag="KRL67_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HBH1521792.1"
                     /translation="MNNEFIILKGCKENNLNNISLKIPKRKITIFTGVSGSGKSSIVF
                     ETIAKESQRQLNERFSTFVRSFLPKYGEVKADCIENLSTPIIIDQSRLGGNSRSTLGT
                     ITDVNSFLRALYSRFGSEYIGNANMFSFNDIDGMCPHCHGLGKKLVPNMNQILDMNKS
                     LNEGAILLSGFGVGSWHWNIFAESGFFDINKRICDYSEVELEKFLHGKAEKIKIENAG
                     QTNMTYEGLMVKFNRLYLSREGEISEATKKKLSKLLIEDRCPICDGRRLNERVYRSLI
                     NGYNIADLTSMQIDELVEVIISIDEPEAQPLIKGIIEKLNSIIDIGLGYLTLDRETSS
                     LSGGESQRIKMVKYLNSNLVDLMYIFDEPSVGLHPKDVYKLNNLLKKLRDKGNTIIVV
                     EHDPDVIKIADHIVDVGPKAGKYGGEVVYEGSYENLLTSGTLTGNALSRFLPIKESVR
                     EHSGYLEVKNCNKNNLKNVSIKIPKGVLTLVTGVAGAGKSTLIKDEFLKQNPNAVLID
                     QSPVSANSRSSLATYSGIMNNIRSIFSKTNGVNASLFSSNSEGACDNCKGSGIVEMNL
                     AFMESIKSTCNVCEGKKFKKEVLEYRFQGKNIIEVLEMSVLDAIEFFNLKQIKTKLQC
                     IEKMGIGYLTLGQTLDTLSGGECQRLKLASELNKESSVYILDEPTTGLHMADIENFIG
                     IIEEIVDNGNTVIIIEHNIDVIKRADWIIEMGPEGGTKGGRVIFEGIPKQLCNNKISL
                     TAKYIV"
     gene            9285..10820
                     /locus_tag="KRL67_000888"
     CDS             9285..10820
                     /locus_tag="KRL67_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HBH1521793.1"
                     /translation="MINDILTLLSERSDFFIKLSIEHLMISIISIIIAIVLGGIIGII
                     VSEFHNSSKPILGTINFLYTIPSISMLGFLIPFSGIGNTTAVIALTIYALLPMVRNTY
                     TGITNVDKNILEAARGMGSTDFQILYKIKLPLALPVIMSGIRNMVIMTIALAGIASFI
                     GAGGLGVAIYRGITTNNTVMTITGSILIALLALIFDFILGLVEKFFQRKRKKKNRKKV
                     FAILGSLILIVTIFTSYLLNPSKTQTIHIATKPMTEQYILGEMLDILIEKNTGLNVEI
                     TQGVGGGTSNIQPAMENGEFDIYPEYTGTGWNMVLKKNELYTESMFNKLQSEYKTKLK
                     MQWAGMYGFSNSYALAVRKEVADKYNLKNISDLKDVSSKLTFGAEYDFYEREDGYTPL
                     CNTYGLNFKDTVDLDIGLKYQAINEGKIDVMPIFTTDGQYSVSDVVVLKDDKNFYPSY
                     QCGNVVRSEVLEENPELSKVFKMVGDILTDKEMAKMNYDVENNNLEPREVAREFLNKK
                     GLL"
     gene            10836..11597
                     /locus_tag="KRL67_000889"
     CDS             10836..11597
                     /locus_tag="KRL67_000889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBH1521794.1"
                     /translation="MTPIIQFKNIKKQYNDKTIIDNLNLDIEKGEFLTVIGSSGSGKT
                     TLLKMINGLILPDGGNILINQIDIKNEDLIKLRRRIGYCVQGSVLFPHMTVEENISYV
                     PNLLSKKDKLEVKSAVNKWMEIVGLPNDMKVRYPSELSGGQQQRVGIARALASSPEIL
                     LMDEPFGAVDEITRKQLQKEIKEIHKKTGVTIIFITHDIYEALILGTKTLVLNHGVVQ
                     QYDTPENILNTPANQFVDQLLNIRKSIIDDEDLKE"
     gene            11938..12807
                     /locus_tag="KRL67_000890"
     CDS             11938..12807
                     /locus_tag="KRL67_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HBH1521795.1"
                     /translation="MDNNLITIEKTLNYIEKHLLDEVLDLDVISKEIGYSKYHLHRMF
                     TSVVGFSVHNYIQRRRLTEAARMLIFTEKPIIEIALISGYDTQRSFSRGFKKMFKRSP
                     NFFRKHQDFLPLQLKFDIHNRKKLRGDMILDVKKVNCADINIVGYSGKVKKGFHIIGK
                     CWRDLHKNKEMIENKVDLGFLIGVSDYSNFKEIENTPTFTYIAGAQVATFGKLPNGME
                     KFVLPKSNYIVFTFIGKNEDSMQNVVEYIYQEWFPNSSCQFNENNLYDFVKYGEKTDE
                     NGLSEIQFWVPII"
     gene            13129..13380
                     /locus_tag="KRL67_000891"
     CDS             13129..13380
                     /locus_tag="KRL67_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3781 domain-containing protein"
                     /protein_id="HBH1521796.1"
                     /translation="MNVENELLKNLDRLHTTELGVVRIKKNLSLETNDVVNWCKTKIE
                     SPNAIINRKGKNWYISVYDCIITVNANSYTIITAHKEKK"
     gene            complement(13693..14343)
                     /locus_tag="KRL67_000892"
     CDS             complement(13693..14343)
                     /locus_tag="KRL67_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HBH1521797.1"
                     /translation="MNIVMINGSPKVNKNNSQYFLSELKQFIQNDNKVFTFKVDNTSK
                     YPDALNAILNCDRLIIAFPLYVDGIPSHLLHFLEECEKFLKTQNKKPIPVYAIVNCGF
                     NEGEHNHLAIQMIEHWCKKINFTWIQGIGIGAGEMFGSLESVPLGKGQKKDLGVALKE
                     LSKNILSDNIHINDSNKSTIFISPNFPRFLYKLMGNHQWNTQAKSNGLTKSDMLKK"
     gene            complement(14340..14843)
                     /locus_tag="KRL67_000893"
     CDS             complement(14340..14843)
                     /locus_tag="KRL67_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HBH1521798.1"
                     /translation="MRLLIHDLTDKELEKLLPKLDNNIKILPNTKEIHKCIGCFGCWI
                     KTPTECVVKDYYSNMGKLINESDGIIIISRCCYGGFSPFVKNVLDKSINYILPYFVIK
                     DNMMRHKSRYDKQINLKAIFYGDCITEAEKETATKLVNGNAINFNIKEHSVCFYENIQ
                     DIRGESI"
     gene            complement(14892..15407)
                     /locus_tag="KRL67_000894"
     CDS             complement(14892..15407)
                     /locus_tag="KRL67_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HBH1521799.1"
                     /translation="MLEYIILGFLMEKELSGYDLKQIMSESTSYFFDASFGSIYPALK
                     RLETKGYIHYHEVIDGSKLKKLYSITNTGKEVFIEWLKKPINFSKTKQDHLVNIFFYK
                     YLPKEIIETNLKMLITEVELLLNKLSKQKIDVEENHCVDQYTYMYATMIYGVDYYNFV
                     INWCKDLLKNL"
     gene            15687..16214
                     /locus_tag="KRL67_000895"
     CDS             15687..16214
                     /locus_tag="KRL67_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HBH1521800.1"
                     /translation="MHEQIDYCERKKIKTIDIKGENMRELLKWVSIINRYNTNYIDRK
                     LADIGINNSQYFYVLHICNNPGITQDTIFEKILVNPSNITRALANLEKEGFITREPGV
                     KDKRTWHLYPTEKSLECYDEILKITNGYVEELLSSFEKEEQELFMSMLKKVAFFAIDM
                     NEEAKLEAKENGRKI"
     gene            16198..17601
                     /locus_tag="KRL67_000896"
     CDS             16198..17601
                     /locus_tag="KRL67_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HBH1521801.1"
                     /translation="MEEKYKEPLEYEKVSKLLTKYAIPSIIAMLVSALYNIVDQIFIG
                     QGVGVLGNAATNVTFPLTTICTAIALLLGIGGASKCSLELGAKNSEKATKAAGNSICL
                     MAVFGISLFIIVSIFLTPLLKFFGSTSEILPYAHTYTKITSIGLPFLIMSTGMSKLIL
                     ADGRPKASMFCMLIGAVINTILNPLFIFVFNMGIAGSALATVIGQLISFGISVHYIKN
                     FQHIKLHKKSFKLDSIIYKHIFALGSSSCFNQLAMTVTQIVMNNTLAYYGARSVYGSE
                     IPLACVGIIIKVNMIFISIIIGISQGMQPIIGYNYGAEKYNRVKEAYKFAVGSATIIS
                     VIAFLCFQLFPRQITSIFGNGNETYFIFAEHYFRTFLFLTFINGVQIVSSTFFTSIGK
                     SVLGLFTSLTRQILFLIPLIVILPMFMGIDGVMFAGPIADAAAAMVCIYFAVHEMKSL
                     TIKQNNLMSNHNKIAEV"
     gene            complement(17848..18159)
                     /locus_tag="KRL67_000897"
     CDS             complement(17848..18159)
                     /locus_tag="KRL67_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH1521802.1"
                     /translation="MKKIIKSFTFCFFLIALFEIYMHQIGQDSKSIILIGLNPILSII
                     STIDSFFVFMDSGMQIPCRTITGSISIYWYIASILSFLIYGIILDLIRIVISKIRNKT
                     K"
     gene            complement(18215..18808)
                     /locus_tag="KRL67_000898"
     CDS             complement(18215..18808)
                     /locus_tag="KRL67_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HBH1521803.1"
                     /translation="MISDSISKRRSIRKYKNQSISHETIEKIIEAGINAPSSKNRQPW
                     RFVVITEKEKESMLKAMSKGIQNEINDNGLLPGSRQHIAGANYTVEIMKQAPVTIFIL
                     NILGKSPLENLSPEERFYEMANMQSIGAAIQNMSLTAVELGLGSLWICDVYFAYRELC
                     EWLNTDSQLVAAISLGYPDEEPSRRPRLQLSDVTEWR"
     gene            19648..20298
                     /locus_tag="KRL67_000899"
     CDS             19648..20298
                     /locus_tag="KRL67_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HBH1521804.1"
                     /translation="MDFISSILQGLLSILFNFTGDFGISIVLITLIVKLVLMPLSLKQ
                     RFSVKKQQELAEKMEHIKEKYKNDTKELEKQIQVSSVESMKSMLGCLIVLVQMPVVYA
                     LYHVCLDMTKEFTTIIIPWIANLGTFDNLFILPCIYTLIMLASNLVNYIPYLKINSQV
                     KFNKQMAVVTTITSLILTVRTPVAIGLYFITSAIYSLIEDICFRIYFNRKNRIVTK"
     gene            20489..21160
                     /locus_tag="KRL67_000900"
     CDS             20489..21160
                     /locus_tag="KRL67_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBH1521805.1"
                     /translation="MAKILVVEDEKRMQSIIVEYMQKGGYTCITADDGVEALTILKSN
                     NIDLMILDIMMPYLDGFSVCRVSREMTNIPIIILTAKGEEEDKLKGYEYGADDYITKP
                     FSPKVLLAKVNALLRRYTTDIPKNSLSLGKIFIMVASRQVYVEDKLIDLTYKEFELLR
                     LFMENPNQVFSREKLLNCIWGYDFEGNTRTVDTHIKTLRKKLGSEGHHIVTLIRSGYK
                     FEVKE"
     gene            21157..22287
                     /locus_tag="KRL67_000901"
     CDS             21157..22287
                     /locus_tag="KRL67_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBH1521806.1"
                     /translation="MNSLRKIVEKLKNVDISNWIKPNFGLYSVRKKVFLLSKLSGALI
                     ILFYLIVEEFSLNSKFEFLIWFIILVIFIMSIDFLLGKFISEPITSINKSAKSMSQLD
                     FSNPCTVNTNDEFGELSRSLNTMSTNLQKALSDLESANIQLEKDVNKERMLLEQRKEL
                     VDTISHEMKTPLGIIRAYTEGLMDEVDEEKKKNYMNVIIEETDRMNNMIVSLLDLSAL
                     EAGVSKLNPERFDFIEFVETVAGRLLIDIPDANFYFTYDLSEYEVFVVADKMRMEQVV
                     ENLIINAKKHVVYDGNLDLSITCENGLLLFEIYNDGRIIEQDEISKIWSKFYRSVQSQ
                     RTGGSGLGLAIVSQILAMQGLEYGVKNRDKGVEFYFMIPIDE"
     gene            22729..23574
                     /locus_tag="KRL67_000902"
     CDS             22729..23574
                     /locus_tag="KRL67_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBH1521807.1"
                     /translation="MIKVEELKFSYGKDKQILHGLNFEVKEGEIFGFLGPNGSGKSTT
                     QKILNGVLKGYNGHVSIFGKEVKAYTESLYQKIGVLFEFPYLYTNLSAIDNLEYFSSF
                     YPKNQRRDIYELLDLLEFKKEFTNKPVSSYSKGMKQRISMARALVSNPKLLFLDEPTS
                     GLDPSGAVLFRKIIEEERKKGTTIFLTTHNMLDADLMCNNVAFIADGKIMAIDKPKNL
                     KMKNSNNKVEVEFLYNGNRDIEIVDIQELESGITFKYDKILSVHSKEPTLEEVFIKYT
                     GRMLV"
     gene            23574..24602
                     /locus_tag="KRL67_000903"
     CDS             23574..24602
                     /locus_tag="KRL67_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBH1521808.1"
                     /translation="MYSKFFTLVKKDFWMMIYGKFFVLTLGSLILYSCYINFGYVKFM
                     DEDSYHNNVYLYNPENISMSASPLIHQVSSIDELKAKLDEDTNAVCIDMSSSKPKVIL
                     HESIKGVDNYRADYALSLLSSEKDHNSKISDNNAIIQINDKDTSEIKQRKEMTCEILF
                     FEIVAIGFLGIASLLFKEKQMGVMRITGILPVKRSLFILSKIFAFLICDLCFTVLLVV
                     FNVGFFEGIKILPQVLVQASILSIIMSLIGIGCSMLLRDFKQFSLIYLVVAVFITTPI
                     FLSANTSFEIDWIKYHPFYHVYSRLKNAFFMNFSKELVYFVVCSLTIIVLFLLVKYIF
                     NKELVREG"
     gene            24605..25618
                     /locus_tag="KRL67_000904"
     CDS             24605..25618
                     /locus_tag="KRL67_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBH1521809.1"
                     /translation="MIGLRYQLKSIKKDKMCIISFFLPVVMAILINFMGTIDISSLGE
                     IRFGIVKDTLPVQTEQWLKKYGMVSVYDTKMHLIREIKDIKTDTIGVVRDGKGIKTVL
                     YGNEINAIKNVGKTLPEMFRLKGDLKDVEVTILPKDSILKEFQNVFIAMILITAMFMG
                     CTFNAMNIISEKEDGISFINEILPQSRSKYIIQKVFLGLLCGCLSSMITVFICLDISI
                     LDMIMMLLLVVFSAFISSLIGLFIGKLSEGLMVGIVYIKIVMILFIGAPLLIYLLGVN
                     TSGIGNLCYIMPSVATFNGIMEIINGNVIIIKDIVILAVHCVAWFMLYIILDKKRKLS
                     FSR"
     gene            complement(26150..27400)
                     /gene="cme"
                     /locus_tag="KRL67_000905"
     CDS             complement(26150..27400)
                     /gene="cme"
                     /locus_tag="KRL67_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter Cme"
                     /protein_id="HBH1521810.1"
                     /translation="MENKNTNSTWKKKVILFLASQYISLFGSSLVQMAIIWYVTLQTS
                     SGVWVTVLTVCSFIPQMIISFFAGVWADYYNRKVLIIVSDSTIAISTLVLILFMMSSN
                     GTTSELIAISVISVIRSLGSGIQTPAVNAMIPQLVPEEHLMRFNGINGSLQSIVQFIV
                     PIVAAAILGFGNKINQVLLIDVFTAIIAIVLLIFIKIPKHDIPKQTEKVSFFADMVSG
                     IKYCFSDKFIRKVLFTYGAFMLLCVPSGFLIGLLIERTFGKGYIYLSIIEMIGFLGMV
                     LGGLIIGVFGGFKNRNKTFFLAILSYAIFSIILGLVTQAWQFFIFMFFTNFSIPIIQA
                     SAMTMLQEHVAPQMLGRVLSLPIIIYTGFIPLGMMIFGPMADVMSINNLIIVAGIILL
                     FFALAIPYSKQFYKQGISKSNESE"
     gene            complement(27486..28232)
                     /locus_tag="KRL67_000906"
     CDS             complement(27486..28232)
                     /locus_tag="KRL67_000906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HBH1521811.1"
                     /translation="MDTYTTSEVADLIGVHPNIVRLYEELNLIPKPKRNTNGYRIFND
                     FHIEQFRFARTALKIEVVQRGLRKDALEIVRTSANRDFDKAISLVNKYLQQLEIEKSN
                     AEEALTIVTQILNGNTPETNKLFLTRKETAEHLNVTIDTLRNWEMNGLLKVKRKQNGY
                     RVYTDEDIKRLKIISALRCANYSLSAILRMLNALSSNPNTNIKTVLDTPKDDDEIISV
                     CDKLITSLKEAEHNAKTMKFHLQIMKKQFS"
     gene            complement(28347..29369)
                     /locus_tag="KRL67_000907"
     CDS             complement(28347..29369)
                     /locus_tag="KRL67_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HBH1521812.1"
                     /translation="MNKNNFLDLIFKKVDPSSVGMSSEKLSQLEPLIISQYSNINGII
                     IARKGKVVFEKYYNGYNADDTFHVASVTKSIISALIGIAIDKGYIKDVDQKVVDFFPE
                     YTCSPSEVCKKAVTIRHLLTMTAPYPFKNMTESLERLCKQKDWIKYTLNQLGQKGEIG
                     TFKYSSAGTHLLSAIITKTTGKSAREFANEYLFKPIGMKIIPDYDMKDFGFEDFFGKN
                     LKGWAKDRQGYSTGGWGLTLSARDMLRFGFLYLNDGIWNNKRIISKSWIEESVKMNSN
                     EYGYLWWLCKKDGVLWYSAMGTGGNAICCIPQYNLVIAIPSKIVFKPRDRWEFIEKYI
                     LTSIID"
     gene            complement(29369..31789)
                     /locus_tag="KRL67_000908"
     CDS             complement(29369..31789)
                     /locus_tag="KRL67_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HBH1521813.1"
                     /translation="MKEIKINGAKIHNLKNIDVSIPKDKLVVATGVSGSGKSSLMFDI
                     VFEEGRKQYLQSLGILAGIDSEDKFDNIQGLAPTIAVQQNIIRQSNPRSTVGTRTNIL
                     NMLTLLYSVEGQIMCTMCDTPVSDNLICNNCGYEEERLRPSYFSYNSSDGMCMKCSGH
                     GAYFEINIEKLVLDKHDTLKQVLDRARITPGYLRVFSKKFNDYLDIQFLQLPEEVRNE
                     VLYGHYENGKKSSSLSKVFHNRYKRGEDLNGVYTMTACSDCNGFKIGEEARRVLLNGK
                     HIGELGKMTILEIDDFLKLLIDQGNLNTFGTNLVNDILSKTRHLIESRLGHLSLYREM
                     STLSGGEMQRLFLNSHLDSEMDSLIYVLDEPTAGLHESEKIDILKSIKKLKDLGNTVI
                     VVEHDKNTIEMAEHIIDIGPKAGVEGGQLVYQGDLEGLLQSDKSLTGQYISGKYPMPN
                     RTSNKNITHTDKIPCITIQNANTNNLKNVTVSLPLGAMVGISGKSGSGKSSLISDTLL
                     PLLRSQFNNHTINNQTNSSESEIGDEDDYAVVETIADRLIGTKHISGYSEISQSPIGK
                     NMNSTPASYIGIWDKIRTLFAGQPESLERGFTAGNFSFNSKGACSKCSGSGYEKVWLG
                     NNLSIDHTCSECQGKRFNDESLSIKYKNKNIHDILNMSVSEAVHFFKDIPNIVSHLNV
                     LEQIGMGYIKLGQPTPTLSGGESQRIKLAKEIGKKRNGNILYVLDEPTTGLSLYDTAK
                     LIQLLDELVSNGNSVIVVEHDIDVLNVCDWIIELGPEGGDKGGYIIAEGSPKVLKENP
                     KSITGRYL"
     gene            complement(31875..32795)
                     /locus_tag="KRL67_000909"
     CDS             complement(31875..32795)
                     /locus_tag="KRL67_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HBH1521814.1"
                     /translation="MTNAKSKKEYLNRICKVQDYIEEHLHEPLSLDELSNIAGFSKFH
                     FHRIFKAIEKETLAQYVNRLKLENATAFLIHRTDMTITDIAHYFGFTDSAVFSRAFKN
                     HYKISPIKYRNNYSKNCKEPYKISQYNRSISKPKCKNTREVKGEIEILELDNMNTIYT
                     RYTGSYGNLTSTFPKLLERLFTYASEQNLLGLENTQILAIYHDNPQFTQEHQLRTSIC
                     MTIPDNIVVKENDNLGNMIIPSGKYLVGHFDISKSEYSDAWDFMYNEWLSDGNYKLRA
                     SFPFEVYLNNPHNHPQNRHLVDIYLPIEPF"
BASE COUNT        12213 a         3903 c         5600 g        11440 t
ORIGIN      
        1 tttaataaaa tataccaaaa tggaaataaa aaataaattt tatgagaatt taataactaa
       61 taattaatat atttaggtta taataaatat gtgcgtaaaa tctaacaata agcaataata
      121 ccgtaaattt aggaggaata atatggatac tttaaaggaa ctttttaaaa taggaagtgg
      181 accttcaagt tctcatacta tgggaccaca aagagcagca gagagattta agaatgaaaa
      241 cccagatgca gaaagtttta gagcaatatt atatggtagt ttggcagcta cagggaaagg
      301 tcatttaact gactatataa ttgaaaaaac tatagcacct aaaaaagttg aaattgtttg
      361 ggaagaagat attataaaag attttcaccc aaacgggatg aagtttgaag ctttagataa
      421 agataaaaat gtaacagctg aatggactgt atattctgta ggtggaggga ccatagcaga
      481 agaaggtcaa agaaacagta aaagcaatag tatatatcac ttagatacta tggatgagat
      541 agtaaaatgg tgcaaagaaa ataataagac tttggtagat tttgtgttgg aatgtgaacc
      601 aaaagacata aaagattata taaaaactat aaaagatgct atgagaaaat ctattgatga
      661 tggactaagt acagacgaaa taataccagg caagttatta ttaaaaagaa gagcaagtac
      721 attttataat gcatataaaa aagataaaag cttctcaaca cttgtatatg catatgcact
      781 agcagcttca gagcaaaacg cttctggtaa tataatagtt acagcaccaa cttgcggttc
      841 tgctggagta ataccaggaa tattctttgc aatgcaggat ttctataatt atgatgacga
      901 aaaaataatt gaagcactac ttgtagctgg aataataggt aatataataa aaactaacgc
      961 ttcaatatct ggagcagagg tagggtgtca aggtgaagtt ggagcagctt gttctatggc
     1021 agcagcagca gtagcatact taaaaggtgg aactatagac catattgaat atgcggcaga
     1081 aatagcactt gaacatcatt taggaatgac ttgtgaccct gtatatggat atgttcaaat
     1141 tccatgtata gaaagaaatg caatggcagc tcaaagagct tatgatgcag ctaattatgc
     1201 attattaact gatggctcac attctgtctc cttagaccaa gttgttgaaa caatgaaaga
     1261 aactggtata gatatgatgg ataagtacaa agaaacagct aagggtggac ttgctaaaca
     1321 tttcttctct tgctaaaaat aaaattttcc aataataatt cctctgtttt gatttatgaa
     1381 acaggggaat tattattaat ggtgaattat ttaataatgc aaaatatttt tatattcaat
     1441 ttagcttttt atttttaatt agtataacat gttatataat attttaatta taaagaaata
     1501 tagaaatatt ttttaataaa ttagaaattg gaggagatta catgagagag gcatcatatt
     1561 tacaagaaca actttataaa cataggaagt cattaaatcc tattgcagag attgggagaa
     1621 aagagtataa gacttcaaaa tatataagag attaccttga taaattagga attgagtatg
     1681 aagtttattt agaaacaggc atagtaggtg taatagaagg taaaagacct aaaaaagata
     1741 ttgcttttag agctgacata gatggtcttc atagtgaaga aggggtaaaa catttatgtg
     1801 gtcatgatgg acatgctact atattacttg gattaattga gtatttaaat gataataaag
     1861 aagacttaaa tgataatata gtatttatat tccaaccagc agaagaaggt cctggggggg
     1921 caaaagcttt aatagatgaa ggagtgctta gaaaatacaa tattgatgaa atatatgggc
     1981 ttcatatata tccagagtta ccagaaggtt atgtaggcac aagagaagga tattttatgg
     2041 ctcaaatagg agatattgac atagaaatag tgtctaaaag tggacatggg gctatgccac
     2101 aaaatggtat tgatgggata gtgatagctt caaactttgt aagctcactt caaactatag
     2161 tatctagaaa tataagtcca atagacaatg cagtgttgac tattggtaga attgaagggg
     2221 gagctagaag aaatataata gctgaaaaca taaagatgga aggtactatg agatgcttca
     2281 acccagaagt gtatgagaaa atgaagctta gagttagaga acttgctaga gggtttggat
     2341 tggcttataa ctgcaaggtt aatataaata ttatagatga ctatattgct gttaaaaatg
     2401 acaaggattt gtatcaagag tttgtagagg ctattggaag tgatactgtt gtagagctag
     2461 agccacttat gatatcagaa gatttttctt attatcaaaa agaagttcca gggttatttt
     2521 ttatgttggg ttcaagaaac gaagaaaaag gatttgtaaa tggtcttcat aatataaact
     2581 ttaattttga tgaaaagata tgtgtaaatg cactgaatgt ttattcaaaa ttattaaaat
     2641 acaaagaagc aatagattag gaagataaat tataaagatt aaaaagcata aatacctaaa
     2701 tttaaaaaaa gtgtattaat aaatacccct tacatatttg tataaattta ttttaattga
     2761 caaaaataca aataagaagg ggtgattttt ttgaaagaaa aaatacaaag agaaaagaag
     2821 ataactaatg gcaagaatac taaaatattg acaaatgatg atataatgaa atatgagata
     2881 gcctcagaac taggtcttat ggataaagtt ggtgaacgtg gatgggcagg acttacagct
     2941 aaggaagcag gaagaattgg aggtatgctt acatcacgca aaaagaggct gaaaaagatt
     3001 gtggaggagc agaataaaaa ggatgagtca atatagcgat tttgcgtttg tatatgatga
     3061 acttatgaat gaagttgatt ataatggttg ggttaaatat atagaagata taatagatag
     3121 tgaaggtgta aaagtaaaaa atatattgga attggcatgt ggcacgggta atcttacaat
     3181 tccattgact aaaaaaaatt acgatatagc tggtatagat atatctgatg agatgcttag
     3241 tgtagcaaga gaaaaggcag aaaaagaagg agtagaatta gttttacttc aacaagatat
     3301 atcagagctt gattttgaaa tatctgattt ggactgtgta ctttgtgctt gtgatggatt
     3361 taattatata acttatgatg atgatttaga aaatgtattt tcaaagacat atgaattatt
     3421 aaagaaagaa ggaatattca tatttgatat aagttctttt tataaattag ccaacatatt
     3481 gggaaataat atgtatggag aaaatagaga agatatagcg tatatgtggc aaaattactt
     3541 tgatgaagaa gaaaatctaa ttgaaatgga acttacattc tttattaaag atgaatgtgg
     3601 aaagtttgaa agatttgaag agatacacca acaaagagca tatactgaag atgagatagt
     3661 tgatatgctt aaaaaatctg gatttaaaga tataaaatat tatggggatt ttacatttga
     3721 caaacctagt gaagaaagcc aaagaatctt ctttgtatgt aaaaaataag atacttttgg
     3781 ctatacaaga taattattaa aaataaccat gatataaaaa tatagaaaat gcagttttat
     3841 agattaaaag taaagtgatg atactttatg acttttgctt tctaataatt ataaagttct
     3901 atagattaaa attaaaatag aatgactatt acttagtaaa actaaaataa aatgggtttt
     3961 gcccaaggtt aatatagata agtatttata ggttaaagtt aaggtagtaa tataataaaa
     4021 taagacaatt aacgggacat ggaggaatac taatgagaga ttatgtacta agagctacat
     4081 caggaaatgg tcaagttaga gcatttgtcg caactactag aaatacagta gaggaagcta
     4141 gaagattaca tgaaactaca aaagtagcaa cagcagcact tggaagaaca ttaacagcca
     4201 catctataat gggattaatg atgaaaaatg atagtgataa attgactgta ataataaaag
     4261 gtggaggtcc aattggaact attatcgcca catcagattc taagggaatg gtaaaaggat
     4321 atgtaggaaa tccgcaagta gaggttgaag actatccaaa tggaaagtta aatgtagcag
     4381 cagcagtagg tacagaaggt gttgtaaaag ttataaaaga tttaggtctt agagaaccat
     4441 ataatgggac ttatccattg gtaagtggag aaattgcaga agattttact tattattttg
     4501 ctgtatcaga gcagactcca tcagtagtag ctttaggtgt tcttacaaaa gaagatgaag
     4561 tagagtttgc tggaggattt atagtgcaat taatgccaga tgcagaagaa gagactattg
     4621 ctaagttaga ggaaaatgta gccaaattac cttctataac taacatgtta aaagaaggaa
     4681 aatcaccaga ggatatatta aatattgttt tagatggttt ggagcctaaa atacttgata
     4741 cctgtgaagt tgggtttatg tgtgaatgtt caaaagaaag agtaaaaact gctttggttg
     4801 caattggtaa aaaatcattg gctcaaataa tagaagaaga taaaaaagca gaagttggtt
     4861 gccagttctg taataagaag tatatgtata gtgaagaaga gttgcttgaa attttaaagg
     4921 aaatgtaggc tataaaaata gttgtattta gataagtaat gtcatagtga tatagtaatg
     4981 tcgtattgat atagtaatta aagtataatt tattaataat agatatattt ctattaaaaa
     5041 ataagtttaa taaattttta agcttaataa attttaagaa ttaagaagct ttctggctgt
     5101 aatatttgca atgttgcagt tagaaggctt ttttatttta ttatagttcg tgtcaaagat
     5161 tgtatgaaac aataaaatat tgtaaaataa tttgtatatt tagacctgat tgacacaaag
     5221 gtttataaga tattttagtt gattatacga aattctatga agtagtaacc ctgtgacttt
     5281 aatcatggga ggttcagaag aaggagaaaa ttttaaatat ggaaagtaaa acgaagtaca
     5341 taggaataag tatattaata ttattgatga tatttttaat tgcaaagaat tttaatggaa
     5401 aacttgaaat acctattcct gagagtaata gtagaaaatt taaatcagca gcagtgtttt
     5461 atcctcaaca tcaagatgat gaggttttat ggggaggaag tgccattgta gatgctataa
     5521 aacagtgtgg tgttgataat gtatatgtgg tattagtatc agatggttct ggagttaacg
     5581 ttttcaaagc taacacaaaa tttagaaact taactaggaa gcaaaaggaa gaacttagga
     5641 ataacgagtt taaatcagct ttgagggagc ttggagtgaa acctcaaaat gtgattatac
     5701 ttgcggatat agataaaaag gaaggcacac attatgaatt aatggaaaag actatactgg
     5761 attttgaata taagtttaaa ggcaatgtga cccatatagc ccatcactat aagtatgatg
     5821 accatataat gcatagaaag aatggggaag ttttaaagaa acttaaacgt gaacgtaaga
     5881 taaaagatga cttgtatttt attaagccaa aatatgtaaa gtatatacca agaaatgaaa
     5941 gagctatcta taaggcactg gattttgatg actataataa ggttaagagt gcttgttatg
     6001 aatataaggt tgtaaattca aagcgtaaaa tgtatggtat aggatatatt tcggcacata
     6061 gttattttga acatctttta aatgaccctg aacttacgtc tgtattaagt gaagattaat
     6121 ttcttataag tagtaagtta tgtttgataa gttgattaga gttttctata gttttgaagg
     6181 tattgaaaat ttagttttat ttaaaaattt aaagatagag gtttttaatg tctggtattt
     6241 taaagttaag ttgaataatt gtatagaatt atttgtgaaa gaagaaacag tgtaaaacat
     6301 agtatcttat ttatggtttt aaaatcatag gcaagatact ttttttatgt aactaatacc
     6361 attttgaggt tattaactta agaattttat ataatttaac actcgtatat aatgatgata
     6421 tttaggtaga atagatgcaa ttacgcttaa ataaaataac tgtatttttt ataaaaatca
     6481 aaccctacac tatactacca gataaaatct acatgctata atttgacctg tgtttataaa
     6541 aaaggagtaa tctaataaaa tagtatttta cttatgtata actaacaact attattacga
     6601 tttgctctaa agaatttaaa gaaaaataat gcaggaggta aaatatgaac aatgaattta
     6661 taattttaaa aggatgcaaa gagaataatt tgaacaatat ttccttaaaa ataccaaaaa
     6721 gaaaaataac catattcaca ggggtgtcgg gttctggaaa gtcatctatt gtatttgaga
     6781 caattgcaaa agaatcacaa cgccaactaa atgaaaggtt tagtacattt gtaagaagtt
     6841 ttttacctaa gtatggagag gttaaagctg attgtataga aaatctgtca acaccaatta
     6901 tcatagacca aagtagactt ggtggaaatt caaggtcaac cctaggtact attacagatg
     6961 taaattcctt tttaagagct ttatattcaa ggtttggtag tgagtatata ggtaatgcta
     7021 atatgttttc ttttaatgat attgatggta tgtgcccaca ctgtcatggg cttggaaaaa
     7081 aactagttcc taatatgaac caaattcttg atatgaataa atcattaaat gaaggtgcta
     7141 tattgctttc agggtttgga gtgggttctt ggcattggaa tatattcgct gaatcaggat
     7201 tttttgatat taataagagg atatgtgatt attcagaagt agaattggaa aagtttttac
     7261 atggtaaagc agaaaagatt aagatagaaa atgctggtca aactaacatg acatatgagg
     7321 gattaatggt taaatttaat cgtctatact taagtagaga gggagaaata tctgaagcta
     7381 ctaagaaaaa attgagtaaa cttcttatag aagatagatg tcctatatgt gatggaagaa
     7441 gacttaatga aagagtatat aggtctttaa ttaatggcta taatatagca gatttaacta
     7501 gtatgcaaat tgatgaattg gttgaagtta ttataagtat agatgagcca gaagcacaac
     7561 ctttaattaa aggaataata gaaaagttaa acagtatcat agatataggt cttggatatt
     7621 taacactgga tagagagaca tcttctctat caggaggaga atctcagcgt ataaagatgg
     7681 ttaagtattt aaatagtaat cttgttgatt taatgtatat ttttgatgaa ccaagtgtag
     7741 gacttcatcc aaaagatgtg tataaattaa ataatctttt aaaaaaactg cgtgataaag
     7801 ggaatacaat tatagttgtt gaacatgacc cagatgttat taagattgca gaccatattg
     7861 tagatgtagg accaaaggca ggtaagtatg gtggagaagt tgtgtatgaa ggcagttatg
     7921 aaaatctact tacaagtggt acgttgacag ggaatgcttt aagtagattt cttcctatta
     7981 aagaaagtgt aagagagcat agtggatatt tagaagttaa aaattgtaat aaaaacaatt
     8041 taaaaaatgt atctattaag atacctaaag gtgtattgac tctagttact ggagtagcag
     8101 gagcaggtaa aagtacgttg attaaggatg aatttttaaa gcaaaatcct aatgctgtac
     8161 ttatagacca aagccctgtt tcagcaaatt caaggtcgag tttggcaaca tatagtggaa
     8221 ttatgaacaa tataagaagc atattttcaa aaacaaatgg cgtaaatgct tctttattca
     8281 gttcaaattc agaaggagct tgtgataact gtaagggttc tggaatagta gaaatgaatc
     8341 ttgcttttat ggagagtata aagagtacct gcaatgtttg tgaaggtaaa aaatttaaaa
     8401 aagaagtttt agaatatagg tttcaaggga agaatataat tgaagttctt gaaatgtctg
     8461 ttttagacgc aattgagttt tttaatttaa aacagattaa aacgaaatta caatgtattg
     8521 aaaaaatggg tattggatac cttactttag gacaaactct tgatactttg tctggaggag
     8581 agtgtcaaag attaaagctt gctagtgaat taaataagga aagttcagtg tatatattag
     8641 atgaaccaac aacaggtctt catatggctg atattgagaa ttttataggt ataattgaag
     8701 aaattgtaga taatggaaat acggtcataa taattgaaca caatatagat gtaattaaaa
     8761 gagctgattg gattattgag atgggtcctg aaggaggaac aaaaggtggg agagtaatat
     8821 ttgaaggtat tccaaaacaa ttatgtaaca ataaaatatc attaacagca aaatatatag
     8881 tataataaat ttatagtgat attttaataa atataatggg tatataaaat gataattaaa
     8941 cttaacagta ataaataatg tgagaaatat tcaaatttag ttaaataaag tgtcagaaat
     9001 ttgtttaaat tttagcattt caacagttct gacctttttt cttattttta tggagaatat
     9061 cgaggatgga gtctctaata gttacttttt tgtgtctact tgcaagtttg attaaaaaat
     9121 ggctatgcac acttgtgact ttagtcatgg gaggttcaga ttataatctt aatatataat
     9181 ttgtgaaata atttgatgac gatttgctga tgtataaaca ataaagatta actacattaa
     9241 aatttaaatc ttgttaagta aaacttataa aaggagagag atgaatgata aatgatattc
     9301 taaccttatt atctgaaaga agtgattttt ttataaagct atccatagaa catttgatga
     9361 tttctattat ttctataata attgcaattg ttttgggtgg tataattggt attattgtaa
     9421 gtgaatttca taattcttct aaacctatat tgggaactat caattttcta tataccattc
     9481 catctatttc tatgttagga tttcttatac cattttctgg aattggaaat actactgctg
     9541 ttattgcact tactatttat gcattattgc ctatggttag aaatacatat actggaatta
     9601 ctaatgtaga taaaaatatt cttgaagcag ctaggggaat gggaagtaca gattttcaga
     9661 tactgtataa aataaaactt ccactggctc tacctgttat tatgtctggt attagaaata
     9721 tggtaattat gacaattgca ttagctggta tcgcttcatt tataggtgct ggaggtttag
     9781 gtgtagccat atatagagga atcacgacaa ataacacagt aatgacaatt actggaagta
     9841 tacttattgc tctgttagct ttaatatttg attttatttt agggctggta gaaaaatttt
     9901 ttcaacgaaa aagaaaaaag aaaaatagga aaaaagtttt tgccattcta ggaagcctta
     9961 ttcttatagt aactatcttt acgtcttatt tacttaatcc ttcaaagact caaactattc
    10021 atattgctac taaacctatg actgaacaat atattcttgg tgagatgttg gatattttaa
    10081 ttgaaaaaaa cacaggtcta aacgtggaaa ttacacaagg tgtaggaggg ggaacatcta
    10141 atatccagcc agctatggag aatggagaat ttgacattta tccagagtat acaggtaccg
    10201 gctggaatat ggtacttaag aaaaatgaat tatatactga aagtatgttt aataaactac
    10261 agtcggaata caaaactaaa cttaaaatgc aatgggctgg tatgtatggt ttttctaata
    10321 gttatgcatt agcagttaga aaagaagttg cagataaata taatttaaaa aatatatcag
    10381 acttaaaaga tgtatcttct aaattgacat ttggagctga atatgacttt tatgagagag
    10441 aggacggata tactccatta tgtaatacat atggactaaa ctttaaagat acagttgatt
    10501 tagatattgg attaaaatat caagctatca atgaaggtaa gattgatgta atgcctatat
    10561 ttacaactga tggtcagtat agtgtttcag atgttgtagt tctaaaagat gataaaaatt
    10621 tctatccatc ttatcaatgt ggaaatgttg ttcgttcaga ggtattagag gaaaatccag
    10681 agctatcaaa ggtatttaag atggttggag atattttgac tgataaagaa atggctaaaa
    10741 tgaattatga cgtagaaaac aacaatttag aacctagaga agttgctcgt gagtttctga
    10801 ataagaaagg tcttttataa attggaggaa atataatgac acctattata caatttaaaa
    10861 atattaaaaa acaatataat gataaaacta taatagataa tcttaattta gatatagaaa
    10921 aaggagagtt tttgactgta attggttcgt ctggttctgg taagacaaca cttttaaaaa
    10981 tgattaatgg tttgattttg ccagatggag gaaatatttt gataaaccaa atagatatca
    11041 aaaacgagga cttaattaaa ttaagaagaa gaataggata ctgtgtacag ggaagtgttc
    11101 tttttcctca tatgactgtt gaagaaaata tttcatatgt accaaatctt ctaagtaaaa
    11161 aggataaatt agaagtaaaa agtgctgtaa acaagtggat ggaaatagtg ggattgccaa
    11221 atgatatgaa agtacgttac ccatcagaat tgtcaggtgg tcagcaacaa cgtgtaggga
    11281 ttgcaagagc cttagcttct tctccagaaa ttttattaat ggatgaacct tttggtgcag
    11341 ttgatgagat tacacgaaag caattacaaa aagagataaa agaaattcat aaaaaaacag
    11401 gagttacgat tatctttatt acgcatgata tttatgaagc tttgattttg ggaacaaaga
    11461 ctttggtatt aaatcatgga gttgttcaac agtatgatac tcctgagaat attttaaata
    11521 ctcctgctaa ccaatttgta gaccagttat tgaatataag aaaatctata atagatgatg
    11581 aggatttaaa agaataaatt tgaatttaaa atcaaattgt tgtgataaaa actactgcct
    11641 tagagctaac ttcaagttgt atgcttgaag tgtacaaagt tagatttaag atatatgagg
    11701 gaatttagca gtttgctgta gacgaggcaa ctgttgaatg tgctttcaat aaatggctag
    11761 cacatgtatc tgatgaagat tataataaat tgaaataaaa agttcaatat acagtagtaa
    11821 ataggcactt caaattgaag tgccttttat gtgtcataaa atgaatcaaa atattagcaa
    11881 agaatgtgaa aaacaaaata aaataatgtg ctatctttaa aataggaggg gtgttatatg
    11941 gataacaatt tgattaccat tgaaaaaact ttaaattata tagaaaaaca tcttttagat
    12001 gaggtgttag atttagatgt gatttctaaa gaaataggct attcaaagta ccatttgcat
    12061 agaatgttta catcagttgt cggatttagt gtacataatt atatacaaag acgtagactt
    12121 actgaagcag cgagaatgct catttttaca gaaaaaccaa ttattgaaat tgctttaatc
    12181 tcggggtatg atacacaacg ctctttttca agagggttta aaaaaatgtt taaacgtagt
    12241 cctaattttt ttagaaaaca tcaagatttt ttaccactac aattaaaatt tgatattcat
    12301 aatagaaaaa aattacgtgg agatatgatt ttagatgtta aaaaagttaa ttgtgcagat
    12361 ataaatattg ttggctattc tggtaaagta aaaaaaggat ttcatattat tggaaaatgc
    12421 tggagggatt tgcataaaaa taaagagatg atagaaaata aggtagattt aggtttttta
    12481 attggagtta gtgattatag caattttaaa gagattgaaa atactcctac atttacatat
    12541 attgcaggtg ctcaagtagc tacttttggc aaattaccta atggaatgga aaaattcgta
    12601 ttaccgaaaa gtaattatat tgtttttact tttattggga aaaatgagga tagtatgcaa
    12661 aatgttgtag aatatatcta tcaagaatgg ttccctaatt caagttgcca gtttaatgaa
    12721 aataatttat atgactttgt aaaatatgga gaaaaaactg atgagaatgg tttgagtgaa
    12781 attcagtttt gggttccaat catttaaaag aaggtattat gtagatggat ttatataggc
    12841 aaagttttat aacagtatgt atttataaat aatttatagt ctgtaggaaa gaaataattt
    12901 tactataaga ttatggtaaa atatacaata ctatgacaaa atagtgttaa ataatagatt
    12961 taattttgca taggaaaagt tctataacgg tgtgtattca taaataattt atagtctgta
    13021 ggaaagaaat agttttacta taagattatg ataaaatata caatactatg ataaaatagt
    13081 actaaataat agatttaatt ttgcatagga taaggtgttt taataaaaat gaatgtagaa
    13141 aacgaattac ttaaaaactt agatagatta catacaacag agttaggtgt tgtaagaatt
    13201 aagaaaaatc tttctttgga gacaaatgat gtagtgaact ggtgtaaaac taaaatagaa
    13261 tctccaaatg ccattattaa tagaaaggga aaaaactggt atataagtgt atatgattgt
    13321 attataacgg taaatgctaa cagttataca atcattacag ctcataaaga aaagaaatga
    13381 aatgaattgc aaaatacaaa ggtcaagagc attttccatt gtttgtagta cctatgaaga
    13441 agtatataaa ataatattac ttttgaataa taatttgaat tttggaggtt gaaaaaatga
    13501 atatactgat ttacagtagg accttgttgg agcgacttgg attttgcaaa aagaataaga
    13561 taaatattag actagataat caatgttgta gtgaattatc ccatgtctag tgaatataaa
    13621 taacataaaa aaccgtcaag atgaatagtt tattcgtgct tgatggtttt tagtatttgt
    13681 tgaagtaaaa ttttatttct ttagcatatc acttttagtc aatccatttg atttagcttg
    13741 agtattccat tgatgattcc ccataagttt atataggaat ctagggaagt taggtgaaat
    13801 aaaaatagtg cttttgttgg aatcatttat atgtatatta tcactaagta tattttttga
    13861 aagttctttt aatgcaactc ctaaatcttt tttttgacct ttacctaaag gtacagattc
    13921 tagtgaacca aacatctccc cagctccaat tccaatacct tgaatccatg taaagttgat
    13981 ttttttacac caatgctcta tcatctgaat tgccaaatga ttatgttcac cttcattgaa
    14041 tccacagtta actattgcat acactggaat aggtttttta ttttgtgttt ttaaaaattt
    14101 ttcacattct tctaaaaaat gaagtaaatg agatggaata ccatctacat aaagaggaaa
    14161 tgcgattatt aatctatcac aattaagtat ggcattaaga gcgtcaggat acttagatgt
    14221 gttatctact ttaaatgtaa aaactttatt atcattttga ataaattgtt ttaattcaga
    14281 taaaaaatac tgtgaattat ttttatttac ttttggacta ccatttatca ttactatatt
    14341 catatggatt cacctcttat atcttgtata ttttcataga aacaaacaga gtgttctttt
    14401 atattgaaat ttatagcatt tccatttact aatttcgtag cagtttcttt ttcagcttct
    14461 gtaatacaat ctccatagaa tatagctttt aaatttatct gtttgtcata tctgctttta
    14521 tgacgcatca tattatcttt tattacaaag tatggaagaa tataattaat gcttttatca
    14581 agtacgtttt tgacaaaagg gctaaaccct ccataacagc atctgcttat tataataatt
    14641 ccatcagatt catttataag tttacccatg ttgctgtaat aatcttttac aacacattca
    14701 gtaggcgttt taatccagca gccaaaacaa ccaatacatt tgtggatttc ttttgtgttt
    14761 ggaagtattt ttatattgtt atcaagcttg ggtaataact tttctaattc tttgtcagtt
    14821 aaatcatgta ttaaaagcct cataaattcc atctcctttt attatgtatg tttaattcta
    14881 aaaattatat cttatagatt tttcagtaaa tctttacacc aattaataac aaaattataa
    14941 tagtcaactc cataaatcat tgttgcatac atatatgtat attggtcaac acaatgattt
    15001 tcttctacat caattttttg tttacttaat ttatttagaa gtaattctac ttcagttatt
    15061 aacattttta agtttgtttc aattatttct ttgggtagat acttgtaaaa gaatatatta
    15121 acaaggtggt cttgctttgt tttagaaaaa tttataggtt tctttaacca ttcaataaat
    15181 acttctttac cagtattagt tatggaatat agttttttta atttgctacc atctataact
    15241 tcgtgatagt gtatatatcc ttttgtttct aatcttttaa gtgcaggata gatacttcca
    15301 aagctggcgt caaaaaagta cgaggtactc tctgacatta tttgttttaa gtcgtatcca
    15361 cttaattctt tttccattaa aaatcctaaa ataatgtatt ctaacatatt gcctcctagt
    15421 atcaattaga tatatatcaa ttcgatatac ttattattgc agataataaa tattacgtca
    15481 agatgctttt taaaataagc taaaaagaat tagaaattat taataaatat gcaaaaaaat
    15541 taatataaaa ggtaattaaa tatcaatgaa gacttaaaaa tagttaaatc aaagtaagag
    15601 tctaatatat aaaatttata gtaactttat ggtttaaata taaacttgtg cgctattgac
    15661 aaagtataaa attagcagta aaataattgc atgagcaaat agattattgc gaacgcaaaa
    15721 aaataaagac aatagatatt aaaggagaga atatgagaga gctattaaaa tgggtttcca
    15781 ttataaatag atacaataca aattatattg acaggaaatt ggcggatatt ggaatcaaca
    15841 atagccaata tttttatgta ttacacatat gtaataatcc aggtattaca caggacacta
    15901 tatttgagaa aattcttgtg aatcctagta atataacaag agctttggca aatttagaaa
    15961 aagaaggctt tataacaaga gaaccaggtg taaaggataa aagaacatgg catttatatc
    16021 ctacagaaaa atctttagaa tgttatgatg aaatactaaa aataacaaat gggtatgttg
    16081 aggagttgtt gagttcattt gagaaagaag agcaagagtt gtttatgtca atgttaaaga
    16141 aagttgcatt ttttgctatt gatatgaatg aagaagcaaa attggaggcg aaggaaaatg
    16201 gaagaaaaat ataaggagcc attggaatat gaaaaagttt caaagttatt aaccaagtac
    16261 gcaattccca gtattatagc aatgttagta agtgctttat ataacattgt tgaccaaata
    16321 tttattgggc aaggtgttgg ggtattgggt aacgctgcta caaatgttac ttttccacta
    16381 acaacaatat gtactgcaat tgcattattg ttaggaattg gaggagcgtc taagtgttca
    16441 ttggaattag gtgctaaaaa ttcagaaaaa gctacaaaag cagcaggaaa ttctatatgc
    16501 ttgatggctg tttttggaat aagtttattt ataatagtat caatattttt aacaccacta
    16561 ttaaaattct ttggctctac atcagaaata ctaccttatg cacacacata tacaaaaatt
    16621 acatcaattg gattaccatt tcttataatg tccactggaa tgagtaagtt gatacttgca
    16681 gatggaagac caaaagcttc tatgttttgt atgttaatag gagcagttat taatacaatt
    16741 ttgaatccat tattcatttt tgtttttaat atgggaattg ctgggtctgc tttagcaact
    16801 gttattgggc aacttatatc ttttggaatt agtgttcatt atataaagaa ttttcaacat
    16861 attaaattac ataagaaaag ttttaaatta gatagtataa tttataaaca tatatttgca
    16921 ctaggttcaa gttcatgttt taatcaatta gcaatgactg taacacagat agttatgaat
    16981 aatacattgg catattatgg ggctaggtca gtttatggaa gcgagattcc acttgcttgt
    17041 gttggtatca ttattaaagt aaatatgatt tttatatcta taattattgg tatatcacaa
    17101 ggtatgcaac caattatagg gtataattat ggcgctgaaa agtataatcg tgtaaaagaa
    17161 gcatataagt ttgcagtggg aagtgcaact attatatctg ttattgcatt cttgtgtttc
    17221 caattattcc caagacaaat tacgtctata tttggtaacg gaaacgagac ttattttata
    17281 tttgcagaac attattttag aactttcttg ttcttaacat ttataaatgg tgttcaaata
    17341 gtatcatcaa ctttctttac atcaattggt aaatcagtgt tgggactttt tacttctttg
    17401 actcgtcaaa ttttattttt aattccattg atagtaattt taccgatgtt tatgggaatt
    17461 gatggagtaa tgtttgcagg accaatagca gatgcagcag cggcaatggt atgtatatac
    17521 tttgcagttc atgaaatgaa atcattgact ataaagcaaa ataatttgat gagtaaccat
    17581 aataagattg cagaggtata agacaatatt taagaagtat agaaaattaa tttaaaaagt
    17641 ataataagct aatttaagaa acatagaaaa ttaatttaaa gaaatacaat aaattaattt
    17701 ttaagtaaca taaattaatg taaataaaat aacgttcaag tgaagattta aaggtgcatt
    17761 ttctggagct aagtatatgt tgtagagtgt tacgtctctc tagcctgtga agtttaatag
    17821 ctcaagaaaa tgcattattt atgatgctta ttttgtttta tttctaattt tagaaataac
    17881 tattctaatt aaatctaaaa ttataccata aataagaaaa gaaagtatag atgctatgta
    17941 ccaataaatc gaaattgacc ctgtaattgt tctacatggg atttgcatgc ctgaatccat
    18001 aaaaacaaaa aaactatcaa tggtagaaat aatagacaaa ataggattta atcctattag
    18061 aataatactt tttgagtctt gaccaatttg gtgcatataa atttcaaata atgcaattaa
    18121 aaaaaaacaa aatgtaaaag atttaataat ttttttcatc aaaatacctc acctaatata
    18181 aattctgttt gcactatttg cttactataa ctatttatct ccattcagtc acatcactta
    18241 attgtaatct aggtcttctt gatggttctt catcaggata acctaaagat attgctgcca
    18301 ctaattgact atctgtattt aaccattcac ataattcacg atatgcaaag tatacatcac
    18361 aaatccaaag actacctaga cctaattcca cagctgtaag cgacatattc tgaattgcag
    18421 ctccaattga ttgcatgttt gccatttcat agaatcgttc ttcaggggat agattttcta
    18481 aaggtgattt acctaatata tttaaaataa aaattgttac tggagcttgt ttcatgattt
    18541 caaccgtgta atttgcacca gcgatatgtt gacgacttcc tggaagaagc ccattatcat
    18601 taatttcatt ttgaatacct ttgctcatgg ctttaagcat agattctttt tccttttcag
    18661 tgatgacaac aaaacgccat ggttgcctgt ttttagatga aggcgcattt attccagctt
    18721 caattatttt ctctatggtt tcatgtgaaa tgctttgatt tttatatttt ctgatacttc
    18781 gtctttttga tatagaatca cttatcattt taagtaacct ccgtttattt tttggaaaaa
    18841 tctatttatt ttctattttt aattatttta gcattaatca gaaaaaaagt cattgatttt
    18901 agtatacaat tattaatgat tgtattaaga attgtatcaa aaagtatgtg attatactaa
    18961 gggattatat tagacattat atttaaaatt gtattaaagg ttgtatttaa tattatatta
    19021 agacttatat atattaagat ttatatatat taggatttat attatattgc gccttatatt
    19081 taagggctat aacttgaaaa taagctataa aaactcaata ttgttttgtt cagagataaa
    19141 aaaaggatac tttaggtgat ttttattaaa aattaagagt gataagatta tatactttat
    19201 aaatgtaaat atgacgaata aaaatgataa atataataat ctatatattt acattaggag
    19261 ttttctatac tataattatt taaaactaaa tataaatgtt ggaattattt aggacttgaa
    19321 attgtctaaa agtaaatata agtgatgaag aggtgatgtg atgagattag atatggatag
    19381 tatgttatat gtactaattt atatgacatt attggtatct atcattggaa gtatattttg
    19441 tttttgtaaa gacctcttaa atcataaaat taagtatgtt ttaataagag acatatttgt
    19501 aaatagaact acatattctg aggaaaggtt gcctaaacag tatatagttt ttcagaaata
    19561 tgatatttgg aggtattgta gtttgtttta atttgaaatg aatctattat aaattctaaa
    19621 gtttaaaaat aaaaaggaga gaataaaatg gatttcattt caagtatatt gcaaggatta
    19681 ttaagtatat tatttaattt tacaggtgat tttggaattt ctattgtact tataacttta
    19741 atagttaaat tagtacttat gccactatca ttaaaacaaa gattttctgt aaaaaaacag
    19801 caagaattag ctgaaaaaat ggaacatata aaagaaaagt ataaaaatga tactaaagaa
    19861 ttggaaaagc aaatacaagt aagttctgta gaaagtatga agagtatgct gggatgcctt
    19921 atagttttgg ttcaaatgcc agtagtatat gctttatatc atgtatgttt agatatgaca
    19981 aaggaattta caacaattat aattccatgg attgctaatt taggtacatt tgataatttg
    20041 tttatattac cttgtatata tacgttaatt atgttagctt caaatttagt gaattatata
    20101 ccatatttaa aaataaattc tcaagtaaag tttaacaaac aaatggctgt ggtgactact
    20161 ataacaagtc ttatactgac tgttagaact ccagtagcca taggtctgta ctttataaca
    20221 agtgcaattt attcactaat tgaagatatt tgttttagaa tatattttaa tcgaaaaaat
    20281 agaatagtga caaaataata taattattat tagaaatatt ttagcctgct aacataggaa
    20341 aagttagtag gctattttta tctatgatat caattccatt aagaatagtc taaaaaatac
    20401 tatcaaaata ctattaaaaa atagttgaaa aataaataaa ttaatataaa ctaaaataaa
    20461 gtctacaaaa aagaggtgat aaatagaaat ggctaaaata ttagttgtag aagatgaaaa
    20521 aaggatgcaa agtattattg tagaatatat gcaaaaagga ggatatactt gtataactgc
    20581 tgatgatgga gtcgaggcac ttacaatatt aaagagcaat aatattgatt taatgatatt
    20641 ggatattatg atgccgtatt tagatggatt ttcagtgtgt agagtttcac gtgaaatgac
    20701 taatataccc attattatat tgacagctaa aggtgaagag gaggacaaat taaaggggta
    20761 tgaatatggt gcagatgatt atataacaaa accatttagt ccaaaagtac ttttagcaaa
    20821 agttaatgct ttgttacgta gatacactac tgatattcca aaaaattcat tgagtttagg
    20881 aaagattttt attatggtgg catcaaggca agtgtatgta gaagataaat tgattgattt
    20941 aacatataaa gaatttgaat tattgcgttt atttatggaa aaccctaatc aagttttttc
    21001 cagagaaaaa cttttaaatt gtatatgggg atatgatttt gaaggaaaca cacgaactgt
    21061 agatacacat ataaaaacct tacgaaaaaa gcttggtagt gaaggacacc atatagttac
    21121 attaattcgt tcaggatata agtttgaggt taaggaatga atagtttaag aaagattgta
    21181 gaaaaactga aaaatgtaga tatttcaaat tggattaaac caaattttgg actatattca
    21241 gttagaaaaa aagtgttttt actttctaaa ttatcagggg cgttaattat attattttat
    21301 ttgattgttg aagaattttc gctaaactct aaatttgaat ttttgatttg gtttataata
    21361 ctggttatat ttattatgag tattgatttt ttattgggaa aatttatttc agaaccaatc
    21421 acatctataa ataaatctgc aaaaagtatg tcccaacttg atttttctaa tccgtgtact
    21481 gtaaatacta atgatgaatt tggagaactt tctaggagct tgaatacaat gtctacaaat
    21541 ctacaaaagg cattatcaga cctagaaagt gcaaatattc aacttgaaaa ggatgtaaat
    21601 aaggaaagaa tgctattgga acaaagaaag gaattggtag atacaatttc acatgaaatg
    21661 aagactccac ttggtattat acgtgcgtat acagaagggc ttatggatga agttgatgaa
    21721 gaaaaaaaga aaaattatat gaatgttatc atagaagaaa ctgatagaat gaacaatatg
    21781 attgtatcat tattagactt atctgcattg gaagcaggag tttctaaact taacccagag
    21841 cgttttgatt ttatagagtt tgttgaaact gtggcaggtc gtcttttaat tgatatacca
    21901 gatgccaatt tttattttac atatgatttg tcagagtatg aagtttttgt agtggcagat
    21961 aaaatgagaa tggaacaagt agtagaaaat ctaattatta atgcaaagaa gcatgttgta
    22021 tatgatggta atttagattt atcaattaca tgtgaaaatg gcttgctttt atttgaaatt
    22081 tataatgatg gtaggattat agagcaagat gagatttcaa agatttggtc taagttttat
    22141 cgtagtgttc aatctcagcg tacaggaggt tcaggtctag ggcttgcaat tgtttcacaa
    22201 atactcgcaa tgcaagggtt agaatatgga gtgaagaatc gcgataaagg agtagaattt
    22261 tattttatga ttccaattga tgaataattt agtcttaatt tataaattat aagtatttga
    22321 tgtaaaatat ttttctttgt agacaataga tatctaaaga gaataaaata aaaaagttta
    22381 ataaaaaaag cttaattaga aagtctaata aaaaaattta ataaaaagct taatgagaag
    22441 tcttgttttc ctgatgaaaa caagattttt tattaagctt tttattattt cacttcgatt
    22501 tcacaagaga gacactttga tttcaccaaa tagaaataaa ataagttaaa taaattaaaa
    22561 aaatataaga ggtaattaaa tacgcttaca agggggggca cttatgaaat ggtatacatg
    22621 gttgactatt atattggtag tattggtttc tattccgctt aaaattaaat ttataaaatg
    22681 gtgggataga cgacaaaaag aaaaaaatga tgaaggaggc tatgatagat gataaaagta
    22741 gaagagttga aattttctta tggaaaagat aaacagatat tgcatggact taattttgaa
    22801 gtaaaagaag gagaaatatt tggatttctt ggacctaatg gttcaggaaa atccacaaca
    22861 caaaaaatct taaatggtgt tttaaaaggt tacaatggtc acgtatccat ttttggtaag
    22921 gaagttaaag catacacaga atctttatat caaaagattg gtgttttatt tgaatttcct
    22981 tatttatata ccaatttaag tgcaattgat aatttagaat atttttcgtc tttttatcct
    23041 aaaaatcaaa ggagagatat atatgaactc ttagatttgt tagagtttaa gaaagagttt
    23101 acaaataagc ctgtatcatc ctattcaaaa ggtatgaagc aacgtattag catggcaaga
    23161 gcattggtaa gtaatccaaa gttgttgttt ttagatgagc ctactagtgg acttgaccca
    23221 tctggagctg tactatttcg taagattatt gaagaagaac gaaaaaaagg aacaactatc
    23281 tttttgacta ctcataatat gctagatgca gatttaatgt gtaacaatgt ggcttttatt
    23341 gcagatggca agattatggc aatagataaa cctaaaaatt taaaaatgaa aaatagcaat
    23401 aataaagttg aagtggaatt tttatataat ggaaatcgag atattgaaat tgtagatata
    23461 caagagttag aatctggaat aacttttaaa tacgataaaa tattaagtgt acattcaaaa
    23521 gaacctacac tagaagaagt ttttattaaa tatacaggaa ggatgttagt gtaatgtatt
    23581 ctaaattttt taccttagta aaaaaagatt tttggatgat gatttatggt aaattttttg
    23641 tactaacact tggctctctt atactttatt cgtgttatat taattttggt tatgtaaagt
    23701 ttatggatga agatagttat cacaataatg tatatctata taatccagag aatataagca
    23761 tgagtgcatc tccattaatt catcaagttt cttctataga tgaattaaaa gcaaaacttg
    23821 atgaagatac aaatgcagtt tgtattgata tgagttccag taagccaaaa gtaattttac
    23881 atgaaagtat taaaggagtt gacaattata gagctgacta tgcattgtca ttattatcat
    23941 cagaaaaaga ccataatagc aaaatatcgg acaacaatgc aataattcaa attaatgata
    24001 aggatacatc tgagataaaa caaagaaaag aaatgacttg tgagatttta ttctttgaaa
    24061 ttgtagctat tggattttta gggattgcct ctttattatt taaagaaaaa cagatggggg
    24121 taatgcgtat tactggtatt ttacctgtaa aaagaagcct ttttatatta tcaaagatat
    24181 ttgctttttt gatatgtgac ctatgtttta cagtgctttt agtagtattt aatgttggat
    24241 tttttgaagg aataaaaata cttccacaag tattagttca agctagtatc ttatcaatta
    24301 ttatgtcttt gattggaata ggatgttcaa tgttattgag agattttaag caattctctc
    24361 ttatatacct tgtggttgct gtattcataa ctacacctat atttctatct gcaaatacat
    24421 cttttgaaat agattggatt aaatatcatc ctttctatca tgtttatagt cggcttaaaa
    24481 atgcgttttt catgaatttt tcaaaagaat tagtttattt tgtagtgtgt tctttgacaa
    24541 taatagttct atttttactt gtaaaatata tttttaataa agagttggtt agggagggat
    24601 agtcttgata ggattacgtt accagttaaa gagtataaaa aaagataaaa tgtgtataat
    24661 ttccttcttt ttaccagttg ttatggcgat tttaataaat tttatgggaa caattgatat
    24721 atcttcatta ggagagatac gttttggtat agtgaaagat acacttcctg tacaaacaga
    24781 gcagtggttg aagaagtatg gtatggtaag tgtatatgac acaaaaatgc atttaatacg
    24841 tgaaattaag gatattaaaa cggacacaat tggagttgtt agagatggta aagggataaa
    24901 aactgtgctt tatggaaatg agattaatgc tataaaaaat gttggaaaga cattgccaga
    24961 gatgtttaga ctaaagggag atttaaaaga tgttgaggtt acaatattgc ctaaagatag
    25021 tattttaaaa gagtttcaaa atgtttttat tgctatgatt ttaattactg ctatgtttat
    25081 gggatgcacc tttaatgcaa tgaatattat atcggaaaaa gaagatggaa tatcatttat
    25141 aaatgaaatt ttaccacaga gtagaagtaa gtatattata caaaaagtat ttttgggtct
    25201 tttatgtggg tgtctatctt caatgataac tgtatttata tgtttggata tttcaatatt
    25261 agatatgata atgatgttgc tactcgttgt attttcagca tttatttcat cacttatagg
    25321 tctatttatt ggaaagttgt cagaaggtct tatggtagga attgtttata ttaaaatagt
    25381 aatgatttta tttataggtg ctcctttgtt aatatatctt ctaggggtta atacaagtgg
    25441 tataggaaat ttatgctata taatgccatc agttgctaca tttaatggta ttatggagat
    25501 tattaatggt aatgtcatta ttataaaaga tattgtaatt ttggctgtac attgtgtagc
    25561 atggtttatg ttgtatatta ttttggataa aaaaagaaag ttatctttta gtagataact
    25621 cagtataatt ataattagga caatttaata ggtgatttaa tatactttgt atagtgtaat
    25681 ttatgtagtg caatttgtat actgtaattt atgtagtgta atagttagtt ttattttata
    25741 attattcaat ataatttaat aagaagttca gtatagttag agctacttag tggaaaattt
    25801 aataggtact taaactatat aaatacttta ttttcctcca tttgtactat aaatttaatt
    25861 gatatataaa tggaggtatt attatatgat ttgataaatt tcatcattat aacttacaag
    25921 taaaggtatt attatatgag atattattat ataaagttaa taaagatagt atcaaaatat
    25981 tatttattat aaagagaggg gctcaaaata aagataaacc tatttttcaa tgttgaaatg
    26041 ataaaaacat atttgatttt atataaaaaa ttttcttcat aaattaaaaa gtccattttg
    26101 agacccctca tttttaacta gataaatcga gttaaactat ttaaatatat tactcagatt
    26161 catttgactt agatattcct tgcttgtaaa attgttttga gtaggggata gccaaagcaa
    26221 aaaataaaag tataatccct gctacgatta ttaaattatt aatagacatt acatctgcca
    26281 ttggaccgaa aatcatcata ccaagaggaa taaaaccagt atatataata ataggcaagc
    26341 taagaactcg acccagcatt tgtggtgcta catgctcttg caacatagtc atagctgatg
    26401 cttgaattat tggaattgaa aaattcgtaa aaaacataaa tataaaaaat tgccaagctt
    26461 gtgttacaag acctagaata attgaaaaaa tagcatagga taatattgct agaaaaaagg
    26521 tcttatttct atttttaaaa ccaccaaaaa ctcctataat caatccgcct aaaaccatac
    26581 ctaaaaatcc aatcatttca ataatagaca ggtatatata gcccttacca aatgttcgtt
    26641 caataagaag tcctataaga aatcctgatg gaacacaaag aagcatgaat gctccataag
    26701 tgaaaagtac ttttctaatg aatttatctg aaaaacagta ctttatccct gataccatat
    26761 ctgcaaaaaa tgagactttt tcagtttgtt ttggtatatc atgtttagga attttgatga
    26821 atatcaataa tacaatagca ataattgcag taaatacatc aattaaaaga acttgattta
    26881 ttttattacc aaatccaagt attgcagcag ccacaatagg aactataaat tgtactatag
    26941 actgtaggct accatttata ccattaaaac gcattaaatg ttcttctggt acaagttgag
    27001 gaatcattgc gtttacagca ggtgtttgta taccacttcc tagtgaacgt ataacagata
    27061 ttactgatat ggctattaat tcacttgttg tgccatttga tgacatcata aatagaatta
    27121 gaactaatgt agatatagca attgttgaat ctgatacaat tatgagtacc tttcgattgt
    27181 agtagtctgc ccatacacca gcaaaaaaag aaattatcat ttgaggtata aaggagcata
    27241 cagttaaaac tgttacccat acacctgatg atgtctgaag tgtgacatac catattattg
    27301 ccatttgtac aagtgatgag ccaaataagg aaatatattg acttgctaaa aaaagaatga
    27361 cttttttctt ccatgtagaa ttagtatttt tgttttccat cctattcctc ctttgatttg
    27421 tgtgttgaat ttttcttgct cttaaggata acacacaaat caaagtctgg agttagagtg
    27481 gagggctagg aaaattgttt tttcataatt tgaagatgaa atttcatggt ttttgcatta
    27541 tgttcagctt ctttcaatga agttatcagt ttgtcacaca ctgagataat ttcgtcatca
    27601 tcttttggag tgtcaaggac agttttaata ttagtattag gattagaaga taaagcattt
    27661 aacattcgta aaatagctga aagagaatag tttgcacaac gaagtgcgct tataatttta
    27721 agtcgcttta tatcttcatc agtatataca cgataaccat tttgttttct ttttactttt
    27781 aacaaaccat tcatttccca atttcttaaa gtatctattg taacatttaa gtgttctgca
    27841 gtttcttttc tagttaaaaa gagtttattt gtttctggag tatttccatt taaaatctgt
    27901 gtaacaatag taagtgcttc ctcggcatta gatttttcta tttctaattg ttgcaagtat
    27961 ttatttacaa gactaatagc cttatcaaag tccctatttg ctgatgttct tacaatctca
    28021 agggcatctt ttcttaatcc cctttgtacc acttcaattt tcaaggctgt acgagcaaat
    28081 ctaaattgtt ctatatgaaa atcattaaag atacgataac cgttggtatt acgcttaggt
    28141 ttaggtatta agtttagttc ttcatataaa cgtactatat taggatgtac accaattaaa
    28201 tctgctacct ctgatgtcgt gtaagtgtcc attagtattc aactcctttt agatatatag
    28261 tactaatcat aataacacca aacaaaagtc tggtgttata gtggagggtt tggttatcta
    28321 agagtaatta gtatgtctaa gagtgattaa tctattatag aagtaagtat atatttttca
    28381 ataaactccc atctatcacg aggtttaaac actattttag atggtatggc tattactaag
    28441 ttatattgtg gaatacaaca aatagcattt cctccagttc ccattgccga ataccacaaa
    28501 actccatctt ttttacatag ccaccacaga taaccatatt cattactatt cattttaact
    28561 gattcttcaa tccacgattt agatataata cgtttattat tccaaatacc atcatttaaa
    28621 tacagaaaac caaatcgcaa catatctcta gcactcaatg taagtcccca gccacctgta
    28681 gaatagccct gtctatcttt tgcccaaccc ttaagatttt ttccaaagaa atcttcaaaa
    28741 ccaaaatctt tcatatcata atctggtata attttcatac caataggttt aaataagtac
    28801 tcattagcaa attcacgagc agattttcca gtagtttttg ttataatagc tgaaagtagg
    28861 tgtgttccag ctgaagaata tttgaaagta ccaatttcac ctttttgacc aagctgattc
    28921 aaggtatatt ttatccaatc tttttgtttg caaagtctct ctaaagactc agtcatattt
    28981 ttaaaagggt atggtgcagt cattgtaagt aggtgacgta tagttactgc ttttttacat
    29041 acttcagaag gactacaagt atattcaggg aaaaaatcaa caaccttttg gtcaacatct
    29101 tttatatatc ctttatctat tgcaatgcct attaaggctg atataatact ttttgtaact
    29161 gatgcaacat gaaaagtatc atcagcatta tatccattat agtacttttc aaagacaact
    29221 tttccttttc tagcaataat tattccattt atattactat attgtgagat tattaaaggt
    29281 tcaagttgtg aaagcttttc tgaactcatg ccaacacttg aagggtctac ttttttaaat
    29341 attaagtcta agaaattgtt tttattcact aaagatacct ccctgttata gattttggat
    29401 tttcttttag aacttttgga gaaccttctg caattatata acctcctttg tcaccacctt
    29461 ctggaccaag ctcaataatc cagtcacaaa catttaatac atctatatca tgttcaacaa
    29521 caattacaga atttccattt gatactagtt catctaataa ttgaatcagc ttagcagtat
    29581 cgtatagact tagaccagta gttggctcat ccagtacata aaggatatta ccatttcgtt
    29641 ttttaccaat ttcttttgca agcttgattc tctgggattc accaccactt aaagttggtg
    29701 taggttgacc taattttata taccccatac ctatttgttc taatacattt aagtgtgaaa
    29761 caatatttgg aatatctttg aagaaatgta cagcttcaga gacactcata tttaaaatat
    29821 catgtatatt tttattttta tatttaatag ataaagattc atcattaaat ctttttccct
    29881 gacattcact gcatgtatgg tctatactta gattatttcc taaccagacc ttttcatatc
    29941 cactaccact acatttagaa caagcacctt tggagttaaa tgagaaatta ccagctgtaa
    30001 atcctcgttc taacgattct ggctgacctg caaatagtgt acgtatctta tcccatatac
    30061 caatataaga agcaggagtt gagttcatat ttttgcctat aggagattga gatatctctg
    30121 aatatccaga aatatgttta gtaccaatca gtctatctgc aatagtttca acaactgcat
    30181 aatcgtcttc atcacctatt tcgctctctg aagaatttgt ttgattattg atagtatggt
    30241 tattaaattg gcttctaaga agaggaagta gggtatctga tataagtgaa ctcttgccac
    30301 tacctgattt tccagatatt ccaaccatag cacccaaagg taatgataca gttacatttt
    30361 taagattatt tgtatttgca ttttgaatag ttatacaagg tattttatca gtatgtgtaa
    30421 tatttttatt gcttgttcta ttaggcattg gatattttcc agatatatat tgtccagtaa
    30481 gtgatttgtc ggattgaagt agaccttcta aatctccttg atatactaat tgaccacctt
    30541 caacaccagc ctttggacca atatcaataa tatgctctgc catctcaatt gtgtttttat
    30601 catgttcaac tactattacc gtattgccta aatctttaag tttctttatt gattttaaga
    30661 tgtctatttt ttctgattca tgtaatccag ctgttggctc atcaagcaca taaattaatg
    30721 agtccatctc tgaatcgaga tgtgaattta aaaatagtct ttgcatttcc ccaccactca
    30781 aagttgacat ttctcggtat aaggatagat gacctaatcg tgattctatt aaatgtcgag
    30841 ttttggataa aatatcattt acaagatttg taccaaatgt gtttaaatta ccttggtcta
    30901 ttaagagttt aaggaaatca tcaatttcta aaatcgtcat ttttccaagt tctccaatat
    30961 gttttccgtt taaaagtact ctacgtgctt cttcaccaat tttaaaacca ttgcaatctg
    31021 aacaggcagt catagtataa actccattta aatcttcgcc ccttttataa cgattgtgaa
    31081 atacctttga taaagaggaa ctttttttac cattttcata atgaccatag agtacttcat
    31141 ttcttacttc ttcaggtaat tgtaagaatt gtatgtctaa ataatcgttg aattttttag
    31201 agaatactct caaatatcct ggtgtaatcc ttgctctatc taagacttgt tttagggtat
    31261 catgcttatc aaggactaat ttttcaatat taatctcaaa atatgcacca tgtccagagc
    31321 atttcataca cattccatct gaagagttgt atgagaaata acttggtctt aatcgttctt
    31381 cttcataccc acaattattg caaattaaat tgtcgcttac aggagtatca cacatagtac
    31441 acattatttg accttctact gaatacaata gtgtaagcat gttgagaata ttggttcttg
    31501 tgcctactgt tgaacgagga ttactttggc gaataatatt ttgctgaaca gctatagtgg
    31561 gagctaaacc ttgaatatta tcgaacttat cctcactatc aatacctgca agtataccta
    31621 aagattgcag atattgttta cgtccttcct caaatacaat gtcaaacata aggctagatt
    31681 tacctgagcc actgacacct gttgcaacaa caagcttatc tttgggaata gaaacatcta
    31741 tatttttaag attgtgtatc tttgctccat taatttttat ttctttcatt ggttcacctc
    31801 attttatcaa ttttatcaaa ccgtaaaata gggatgtatt aatccttaat acaagaatag
    31861 attaggtgag gttattaaaa aggttctata ggtaaatata tatcgaccaa gtgtctattt
    31921 tgagggtgat tgtgtgggtt gttaagatat acttcaaaag ggaaggaggc acgtaatttg
    31981 taattaccat ccgatagcca ttcattatac ataaaatccc acgcatcact atattcagat
    32041 tttgaaatat caaaatgtcc cacaagatat tttccagaag gtattatcat attgccaaga
    32101 ttgtcattct ctttgacaac aatgttatct ggaatggtca tgcaaatact agtccttaat
    32161 tgatgctcct gtgtaaattg tggattatca tgataaatgg ctaatatttg tgtgttttca
    32221 agtccaagta gattctgctc acttgcatat gtgaataacc gttctaaaag ttttgggaaa
    32281 gtactggtta gattaccata tgaaccagta tatctagtgt aaatagtatt catattatca
    32341 agttctagaa tttcaatttc acctttaacc tctctagtat tcttacattt aggttttgat
    32401 atgcttctat tgtactgaga aattttatat ggttctttgc aattcttgct ataattattt
    32461 ctatatttta taggacttat tttatagtgg tttttaaatg ctctcgagaa aactgctgaa
    32521 tcagtaaagc caaagtagtg tgcaatatca gtaatggtca tgtcagttcg atgaataaga
    32581 aaagcagttg cattttctag ttttagccta tttacatact gtgccaaagt ttctttttct
    32641 attgctttga agattctatg aaaatgaaac tttgagaaac ctgctatatt agaaagttcg
    32701 tcaagggaaa gaggttcatg taaatgttct tctatatagt cttgcacttt gcaaatacga
    32761 tttagatatt cttttttact tttagcgttt gtcatagata gccctcctta ttgtgaaaaa
    32821 tgtgtattaa ttaatttata gtaatatagt aataaaattc tatgtttttg taatttatga
    32881 taaataatat agagttcaat tttttataat taataaaata tatttataac caacaaaaga
    32941 tataagatgt cttatatttg tctttgcata taagtatagt cttcgtaata attagtatac
    33001 catagtgatt tatttttaat tgtgtattta tataaataca caacttatga tatggacggg
    33061 ttattatatg aataagctgt aattaaataa attacaatat aaaccagtta aaacataagc
    33121 tagagaatat tgtggaattt attacctagc acaaga
//
LOCUS       DAEPXF010000021        32849 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain HB27
            SAMN13376389-rid12479423.denovo.022, whole genome shotgun sequence.
ACCESSION   DAEPXF010000021 DAEPXF010000000
VERSION     DAEPXF010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13376389
            Sequence Read Archive: SRR10518432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 32849)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32849)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001069624.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001069624.1
            Genome Coverage       :: 315x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/15/2021 01:54:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,165
            CDSs (total)                      :: 4,076
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 89
            rRNAs                             :: 10, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 10 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 19 of 69
            Pseudo Genes (incomplete)         :: 44 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 10 of 69
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32849
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13376389-rid12479423.denovo.022"
                     /strain="HB27"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="China: Hebei"
                     /collection_date="2017"
     gene            61..1917
                     /locus_tag="KRL67_000910"
     CDS             61..1917
                     /locus_tag="KRL67_000910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HBH1521815.1"
                     /translation="MDTHNKYVNFIKNIPVPFLYCRIVKRQEDIEYRVEYISKGMGKV
                     LQLEEGICDKNILDVLPVFKSKKYFKELFSNEVDCIKRYIPTLKNWINIKKQIIGDSY
                     IILYFGKIVFDYRQIIDSFDKKEKVAYIKDEEGIYIDCSENLIPILNNNIKTTKDIFG
                     KNDIEVWGENTGKLFRDDYREGVSSKKRFLQNLFEYEETFFMVEKYFLYDEDELLGTI
                     GIIDNIIYSDYSNRNYNSKDLMKMIEHSIPENMFYKDVYGNYIGFNSGFLNLACMNKE
                     ELLGKNSYKISEEEVLIDKIFESDKGVIENKKVVTFELNVSINDENKCIEITKRPFFD
                     SYGSVIGIIGTARDISRRKRLEEEMDKTRMEFFANLSHELRTPINLISSSLQVIEKKE
                     ADLIESNDTLKRNLGIIKQNGNRILRLVNNVIDFTKMQSGYLDFKPEESDIIAFIEEI
                     CMSVADFASQNNIQLTFDTEIEEFSMLFDSEKLERIILNLLSNGIKYNKEDGKINIFL
                     YVKDNIFNMKISDSGIGIPKEKIDKIFNRFEQIDNELSYRVKGSGIGLSLVKSLVELH
                     EGSISLKSQLGIGSEFIVSLPVRSKNNIEKYNHKREISNELSKKLEIEFSDL"
     misc_binding    1999..2239
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     misc_binding    2305..2540
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            2625..3842
                     /locus_tag="KRL67_000911"
     CDS             2625..3842
                     /locus_tag="KRL67_000911"
                     /EC_number="1.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HBH1521816.1"
                     /translation="MENKVKIGVLGYGVVGSGLIDIIDNNKEKRNIEIVGILVNNLEK
                     HKDKKYSNIITNNIDDIFNKDIDILVEVMGGLEPSLSYIKKALNNKIHVVTANKDLLA
                     ECGDELAKLASENKVSIKFEASVAGGIPVLKPIIESLEGNNIDSINAILNGTTNFILS
                     KMYDENLSYDMALRQAQELGFAEANPESDVLGYDAARKLSILSTLAYDNRVYWKDVYL
                     EGITDIDEKDIEYAKKLNCKIKLIGQSKYENDKVSAFVRPVLVEKDNILARIDNEFNA
                     VIVNGDSVGEVSFVGKGAGSLATGSAVYSDVIDIIDNRVSSIDSFTKDKIQVNKIVRE
                     KCGALLRFKKCNKDEILNIVENCLVKFDILNDDDELAIMVYADSEYEINNSLCLIKDK
                     GYCEKMNKMLKIS"
     gene            3959..4441
                     /gene="cdeM"
                     /locus_tag="KRL67_000912"
     CDS             3959..4441
                     /gene="cdeM"
                     /locus_tag="KRL67_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exosporium morphogenetic protein CdeM"
                     /protein_id="HBH1521817.1"
                     /translation="MENKKCYSEDWYERGESTAKWFQNDREEYERAAYDEDRERRGSN
                     CGCSDSGENRPRNCERFRREAEIREREAREAFCESSEKKKEALAYECEARKLWEEAEK
                     YWDEYSKYNYQGIEYLAEAARLFDEGMECEARRNGNNGGNNNNCCHKCHKCNCNCCRK
                     "
     gene            4706..5971
                     /gene="hisD"
                     /locus_tag="KRL67_000913"
     CDS             4706..5971
                     /gene="hisD"
                     /locus_tag="KRL67_000913"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HBH1521818.1"
                     /translation="MKSIKTSSMKEKVDEKILREQVSSIIEDIRNNKDVALKKYNEKF
                     DRNTRDEFRITKEEIKEAYKHVDDEFINNLKIAAKNIREFAKAQKLSFENPFEKEIYP
                     GVILGQTNIPIESCLAYVPGGQYPLFSTALMLIIPAKVAGVKRIVACSPTMKNTEKIN
                     PKTLVAMDIAGADEIYATGGVQAIAAFTYGTEKINPVDIIVGPGNKFVTEAKRQCYGQ
                     VGIDFVAGPSEVLIIADETSNPVYIAADLLAQCEHDLNARGILLTNSLEIAQEVEKNI
                     ESMLKDLPTKDIAYSSWENNGEIILVDDMEEAIKISNFYAPEHLEVAVNEYNDICDRL
                     TNYGSLFIGNLSAEVFGDYVSGTNHTLPTLKASRYTGGVWVGTFIKTCTKQIFNEKAI
                     QSLAPVAEKLAKEEGLYAHAKAAEVRFKK"
     gene            complement(6176..6928)
                     /locus_tag="KRL67_000914"
     CDS             complement(6176..6928)
                     /locus_tag="KRL67_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBH1521819.1"
                     /translation="MSYFQYQGNSCFYKEYGQGKPIIFLHGNTGSSKMFKALMPLYVE
                     NFRCILIDFLGNGQSDRVSQFSPDMWHDEALQTIALIEHLNCGKVGLIGTSGGAWAAV
                     NTGLERPDLVEAIIADSFDGRTLNDNFIDNLITGREKSKQDIQARKFYEWCQGKDWEN
                     VVNLDTKALLQCANEKRPLFHKELCKLEMPILFTGSKEDEMCRHNLEEEYKQMATFIS
                     KASIHIFSQGGHPAILTNADEFAQLAKAFFKY"
     gene            complement(7046..7315)
                     /locus_tag="KRL67_000915"
     CDS             complement(7046..7315)
                     /locus_tag="KRL67_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH1521820.1"
                     /translation="MSYQNSIDLLPKELIEQVQEYIDGKVIYIPKKQEHKNHWGENTN
                     TKQVLASRNSQICINFQNGMSIKQLSEKYFLTEKSIQRILKQQNL"
     gene            7790..8884
                     /locus_tag="KRL67_000916"
     CDS             7790..8884
                     /locus_tag="KRL67_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HBH1521821.1"
                     /translation="MKNIVGKPQTIKKVNEDLIKNIIKNEGPITKPEISSITELSLAT
                     VNKIVEQLALKNEVKVSGLSESTGGRRAQLFEINANLEHIIALYYYRNCCIGVVANLL
                     GEIIYQEEFNIRMDTYENVNQDTFFVIDTLIKKEKGNNIRAIGIGVPGVVKKGVVKNI
                     PTIKSWEGINLENLIKSRYNTKVFIENDTNITTIGIYQKQYKDKYKNMALMYLEDGIG
                     SGIVINGELYLGSTNFAGELSYLNIEFGKYNSNNLNLEDYIMSLRKDYLESKDNKFKK
                     EILSVISNFIRTLVCVLNPQAMIIQCSILTKSDMEFIEKDVSNSVGEENFPKLIKVDS
                     LREGSIHGVISMCLKETDYQYSLSNKKGGY"
     gene            8886..9836
                     /locus_tag="KRL67_000917"
     CDS             8886..9836
                     /locus_tag="KRL67_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine kinase"
                     /protein_id="HBH1521822.1"
                     /translation="MKYLVSIDGGGTKTKFCVSDLDGNILKEHTTGSTNYKSVGIKQT
                     YENINNGFKKILKDLYIDYDDIEYTVFGISGCDSPNDYKIIMDEILKIGINKEKIYLA
                     NDAVLAFYAQADSPGLVIVAGTGSIILGIKEDGEIYRVGGWGYNFSDLGSGYDIGRKL
                     LKKVLLYCDECHEYSDLFNCVLDFFGANSFEQLAYMITDINNNVEIANLASLVIDCAK
                     QGDKLAIEILRESSTELSKLAQVILSKISNPMKRDKSEINIVLSGGTLNKTVYAEMLM
                     NNLKIENKDKNLKFISQENQPVYGGIRLAMALANRRVKHG"
     gene            9829..11013
                     /locus_tag="KRL67_000918"
     CDS             9829..11013
                     /locus_tag="KRL67_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH1521823.1"
                     /translation="MDRKKVIVQIFTGGFKTKEIKFNDIKCKLLELLDCIDIEKVIMG
                     WSVDEELYVETKKLLNKYNVELYLWLPCFSEIGLLEKSNLLIDYTGKEVKGYALQEDE
                     NFEFYCPNNKENIKNIKKIYSTYFSDIKFDGIFLDKIRYGSFSNKLNGVFNCFCKDCL
                     NIYEKNNIDKKSLMIEMNNMSKGVTDNEDTPFGITKYINGKYKFKNKLWEDFFETKNQ
                     NIYKALLEISSYFKSKGLKIGIDTFSPFISYFTGQDLEKLQEVADFIKPMMYRTTKAP
                     AGLPFEFERFIDESIYKNKEIAKRNFLEILGIKEYEGDVYPIEFVLEEIDFMTRSLNK
                     DIYVGVEINKKEIIAPVTPKYISENISNINKTTVKGYVLSWDLLSAPKENIDEIIKWF
                     RN"
     gene            11026..12522
                     /locus_tag="KRL67_000919"
     CDS             11026..12522
                     /locus_tag="KRL67_000919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HBH1521824.1"
                     /translation="MSKILEMQQIYKSFGKVDVLKGINFELEKGEVRALLGANGAGKS
                     TLIKILGGVHSQTKGKVILAGNEIMFKNPHHSKENGISIIYQELSLIPTLTVLENVFL
                     GRELLKGSVFLNKKDMLEEYMKICEDFDFNIDPHIIVSNLSVANQQMVEIMKAVSCNS
                     EIIIMDEPTTSLTNNEKEGLFKIISRLKSKGKTIVYISHILEEVFKVCDSASIMKNGI
                     MIGTYDVEDLTKLKITQLMTGNDNHKLVEKKDYTFAKYEELPVLEVKNISWSNKIKNV
                     SFKVYRGEVVGLAGLVGSRRTEIINLIFGIDNKDSGDIFINGKKVDITSPKVAIKNKI
                     GLIPEDRKNLGLILEQAIYTNSSSVQIDKFKKFLFIDRNKEVEFSKKGVKELGIKVSD
                     VKQEVKELSGGNQQKVVVSKWLEKDLDLIIYDEPTKGIDIAAKEDIFNTIKEFSSKGI
                     GVIFISSDLEEVIRVSDRILVTREGSIVDDLKNENIQVQDIMNSIFNV"
     gene            12546..13496
                     /locus_tag="KRL67_000920"
     CDS             12546..13496
                     /locus_tag="KRL67_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBH1521825.1"
                     /translation="MKKAFEKLNLRDYGVLLGFLALCIAISIATPSFLGKQNILNLLR
                     QSSIIGIISAGMTFVIISGNFDISVGAVSALAGAITMKFATGGTNLFASMLLGIAICA
                     VIGLINGIVVAKINVPSLIATMAMVTIVRGLLLMLTGGYPITENIPILDYIGNGYLFN
                     IPIPVVIFFIVVLASFIVLNKTKFGRYVYSVGGNQEASKLNGINVDSHKVKVFIINAV
                     LAGIAGIVLTGRLGTATAIAGEGYDMDAIASVVIGGTSVAGGSGSVLKTVIGVLLMSV
                     INNSFNLLGIDVYFQYIFKGLIILAAVGFDSYSKKKLASR"
     gene            13520..14482
                     /locus_tag="KRL67_000921"
     CDS             13520..14482
                     /locus_tag="KRL67_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HBH1521826.1"
                     /translation="MKKLMKHLALLISFIMIFSLVGCSNNGSKGKEGEKKIAVLLPGS
                     TGYFVATKQGIDAKSKELGVSVEYADAQWDASKQLSQAEDFMAKGVDMIALCGVDSAV
                     SERIVKAANDSDVPIIAFTNSIGSNPTGEFKGLVTYIGQNEEETGALTGKIAKNLLGE
                     AGGKAVLIEGVPGTTPQVLRKKGLEKELKDSNIEIVYNQTSNWEKEQALKVTEDLIQK
                     KTDFNVIICQDDNSATGAGQALKDAGIKDKVKVIGLGGSKDGLKAITDGLIDGTTYMS
                     AVKEGGLVIEKASKFLKGEKIEPVTQIKQVEVNKDNISEFKGEW"
     gene            14848..15480
                     /locus_tag="KRL67_000922"
     CDS             14848..15480
                     /locus_tag="KRL67_000922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HBH1521827.1"
                     /translation="MARRLFLERIGLIFLGSAILAFGVYNFYYLNNITEGGVLGILLL
                     LKNLFNIQPAIANVVIDGLLLLVGYKFFGKKFLIYSIVASITFSVLYDLFEAIGPLVP
                     QSQNMLLSTILAGVTVGTGVGIVVKAGCASGGDDALALVISKTTSLNIGQVYLATDVI
                     VLLLSLFYLSAFDIFYSLIAVTISGKVIDFIYYHGKSLDMDISNDIVPEC"
     gene            15891..15983
                     /gene="kdpF"
                     /locus_tag="KRL67_000923"
     CDS             15891..15983
                     /gene="kdpF"
                     /locus_tag="KRL67_000923"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR02115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit F"
                     /protein_id="HBH1521828.1"
                     /translation="MEGLPMWFLAVVIIMLIIYLVYALINPEKF"
     gene            16024..17706
                     /gene="kdpA"
                     /locus_tag="KRL67_000924"
     CDS             16024..17706
                     /gene="kdpA"
                     /locus_tag="KRL67_000924"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015391009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpA"
                     /protein_id="HBH1521829.1"
                     /translation="MFMVQIVIVLAIFMILVIPMGKYLYHIATNQKTFGDRLFDKVDN
                     FIYKVCSIDKKKEMNWKQYALALLFTNAVMVFIGYIILRTQSMHIFNPSGIKSMEQGL
                     SFNTIISFMTNTNLQHYSGESGLSYFSQMTVIIYMMFTSAATGYAAAMAFVRGLVGKK
                     KTLGNFYVDLIRITTRVLLPGALIIGLILVTQGVPQTFAGTETVTTIEGKLQDIARGP
                     VAALESIKHLGTNGGGFFGSNSSHPFENPTIISNIVEILSMMILPGACVVAFGHMIKN
                     KKQGWVVFGAMSIIFLIGLVVCFKAESAGNPILSQLGLNQSMGSMEGKEVRFGIAQSS
                     LFTTVTTSFTTGTVNNMHDTLTPLGGLVPLLNMMLNVVFGGKGVGLMNMLMYAIIAVF
                     LCGLMVGRTPEFLTKKIEGKEMKLIALLIILHPLLILMFSGLSVAIPAGLEGISNPGF
                     HGLSQVLYEFASSAANNGSGFEGLGDNTMFWNMTTGIVMFFGRYVSIIVLLAISSLLA
                     SKKAVNESIGTLRTDNFTFTIVLVLVVLIVGALTFFPALALGPISEHLVLWH"
     gene            17731..19788
                     /gene="kdpB"
                     /locus_tag="KRL67_000925"
     CDS             17731..19788
                     /gene="kdpB"
                     /locus_tag="KRL67_000925"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010966942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpB"
                     /protein_id="HBH1521830.1"
                     /translation="METKKTKFITKDIFKTSIIEAFKKLNPKYMMKNPVMFVVEIGFI
                     VTLILTIVPSLFGDEGNNLRAYNGIVTIILFITVLFANFAEAVAEGRGKAQADTLKKT
                     RKDTVARVIDTLGNEKMISASELKLGDIVLVNAGEVIPNDGEVIEGIASVDESAITGE
                     SAPVMRESGGDFASVTGGTTVVSDWLKIRITTKPGESFLDKMIALVEGASRQKTPNEI
                     ALNTLLVGLTLIFMVVLISLYPMANYAGVKIPISTMIALLVCLIPTTIGGLLSAIGIA
                     GMDRVTRFNVIAMSGKAVEACGDVDTMILDKTGTITYGNRLASEFIAVGNADSKDLIN
                     YSVMCSLKDDTPEGKSIVDLGMKLGSTGKTKEAEEAEFVEFSAQTRMSGINLADGRAI
                     RKGAYDSIIKRVKESGGTVPEDLEENVNRVAKLGGTPLVVCVDNEIYGIIYLKDTVKS
                     GLVERFARLREIGIKTIMCTGDNPLTAATIAREAGVDGFIAECKPEDKIDAIKKEQLE
                     GKIVAMTGDGTNDAPALAQADVGLAMNSGTTSAKEAANMVDLDSDPTKILEVVEIGKQ
                     LLITRGALTTFSIANDVAKYFAIIPAIFTLAIPQMEILNVMKLSTSFSAILSALIFNA
                     IIIPCLIPLAMKGVKYRPMKSQAMLMRNMLIFGLGGVIVPFLGIKLIDLLITPLVMVL
                     NLG"
     gene            19807..20433
                     /locus_tag="KRL67_000926"
     CDS             19807..20433
                     /locus_tag="KRL67_000926"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit C"
                     /protein_id="HBH1521831.1"
                     /translation="MKTLKPALLVSIVLLVVCGLVYPLVLTSVSQVAFKDKANGSMIE
                     VNGVKVGSELIGQSFTDARFFKGRVSSVNYNTYTKEDLVPDKDGNTSYGGVSSGSFNY
                     GATNPELHDRVQKDIKQFLKDNPTVKKEDIPTDLLTASGSGLDPNISPASAKIQIPAV
                     SKASGISESKLQKIVDDNTSKKLFGVLGEDRVNVLKVNVEVAKILGLI"
     gene            20670..21578
                     /gene="cysK"
                     /locus_tag="KRL67_000927"
     CDS             20670..21578
                     /gene="cysK"
                     /locus_tag="KRL67_000927"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HBH1521832.1"
                     /translation="MLYNNALELIGNTPVVRLNNLGCTNGCSNIYVKLEKVNPAGSIK
                     DRAVYEMLEGLEKRGELKKGDVLVEATSGNTGIALSMIGKLKGYEVVIVMPETMSVER
                     RDLMKAYGANLVLTDGKAGMAGSIEKANELLKENPNYKSLKQFENKDNPNAHYKTTAV
                     EIMNDVKDLDIFVCGVGTGGTLIGTARYLKEQNPNIRVIALEPKKSPAISENKTGPHK
                     IQGIGANFVPENYDSSVVDEVILVDDEDAFDTVKLLAAKEGILVGISSGANIFGAIEM
                     AKKYPDKKIVTVAPDGVDKYMSMGIF"
     gene            21605..22195
                     /gene="cysE"
                     /locus_tag="KRL67_000928"
     CDS             21605..22195
                     /gene="cysE"
                     /locus_tag="KRL67_000928"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HBH1521833.1"
                     /translation="MFKKINKDIEYIMKNDPAARSKIEVFLLYPSVHAMIMHRMAHAL
                     YKKKKLFTARLISQISRFMTGIEIHPGAKMGEGILIDHGMGVVIGETAEVGNRVTIYQ
                     GATLGATGKDTGKRHPTVGDDVLIGAGTKILGPLNIGSNSKIGANSVVVKDVPNGATV
                     VGIPAKIVKIRNLEPVKKNKKEVGYEYDELDNVYYI"
     gene            22356..22544
                     /locus_tag="KRL67_000929"
     CDS             22356..22544
                     /locus_tag="KRL67_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HBH1521834.1"
                     /translation="MKANVNQDTCIGCGLCPSICPEVFDMKDDGKSHVIVDEVPSDAE
                     ESAAEARESCPVDAIDVH"
     gene            complement(22598..23467)
                     /locus_tag="KRL67_000930"
     CDS             complement(22598..23467)
                     /locus_tag="KRL67_000930"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl aminopeptidase"
                     /protein_id="HBH1521835.1"
                     /translation="MKLNRNDSCWCGSTKKYKKCHMDMDNKIDSFAKLGHIVPTHDII
                     KNESQIEEIKKSAVINTKILDYISDKIEIGMSTEDINKLVHDFTLQNGAIPAPLDYNG
                     FPKSVCTSVNNEVCHGIPSKDIILKDGDIINVDVSTIFNGYYSDASRMFMIGNVNENA
                     KKLVQVTKECVQKGLEVAKPWGFLGDIGDAINSYAKKNGYSVVREIGGHGVGLSFHED
                     PFVSYVSKKGTEMLLVPGMIFTIEPMVNEGTHEIFVDEENNWTIYTKDGKLSAQWECM
                     VLVTETGVEILTE"
     gene            complement(23664..24908)
                     /locus_tag="KRL67_000931"
     CDS             complement(23664..24908)
                     /locus_tag="KRL67_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH1521836.1"
                     /translation="MENNLNTAKRNNTELSQNDSLKVNLQIGVDFTLLSKSLKAFYKK
                     EKDFYCIALAPCNVSSEKANRKISIYDMIKEINILIESITDKNELLSEELFTNNLSEF
                     CDKSLDEIYIDLKQAYLYIKKNTDSLNIEDSIEYVFDVDISNDIKAKENPLINFNSLS
                     LVVWSGETKHILQQMNILDLEDIHSYSMENNTNITIYENDTVQYKFDEESEDIKDSSI
                     DIIEQMLILPDDLNYNKDEVENMKSRLAKINIKYLQTLKEKDIKIKLINSNLTDEPEF
                     SDLKYQLPPCWVRSGKTWKDVPGIYRNNSIVAKIGYSNPSYANVHSSKNLELHETAHA
                     IDKNVLNKKSNSEEFIEVFAQERYKLYDPKQVAHAYISKFIEEFFAESFVHYYLDEDS
                     KNTLKENCPLTYDFLEKLELNY"
     gene            25198..25509
                     /locus_tag="KRL67_000932"
     CDS             25198..25509
                     /locus_tag="KRL67_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HBH1521837.1"
                     /translation="MYFTYIIRCKDDSLYTGYTSNIVRRMNEHKLGINSKYTRAKGFK
                     KLEVYFVTNTKSNAMKLEYYIKKLTRNKKLSIIKNPSILINLIDNKEDYIIGNEIEQL
                     T"
     regulatory      25602..25694
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            25794..26588
                     /gene="thiD"
                     /locus_tag="KRL67_000933"
     CDS             25794..26588
                     /gene="thiD"
                     /locus_tag="KRL67_000933"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HBH1521838.1"
                     /translation="MSNYKIPTLTIAGSDSSGGAGIQADLKTFSAIGTYGMSVITAVT
                     AQNTKGVFDVEDLNKKIIKKQIEAVFEDIPPRAVKIGMVSSPEIILEIVENLKKYNPK
                     YLVVDPVMISKSGYYLLKPEAKENLIKHLIPLAYIITPNIPEAEEITGIKIHNVDDMK
                     RVGKEILQLGPKFVLMKGGHLDGEAVDILVGKNIFKVYKSERIDKKNTHGTGCTLSSA
                     ITSYLALGYEITEAVNLSKIYITEAIKRSFDIGHGVGPVHHFYKFE"
     gene            26612..27400
                     /gene="thiM"
                     /locus_tag="KRL67_000934"
     CDS             26612..27400
                     /gene="thiM"
                     /locus_tag="KRL67_000934"
                     /EC_number="2.7.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyethylthiazole kinase"
                     /protein_id="HBH1521839.1"
                     /translation="MYNLIKDVKKLNPLVIHYTNNVTINDCANVTLAVGASPLMSFSY
                     EEVEEMVSVANSVVINIGTMNSNMLDLFLLAGKAANKYNKPVVLDPVGVFASKARAEL
                     TSRLLNEVKFSVVKGNVSEIKFIGGFNVRGKGVDSFDEEEDSTEIIRKIAEKLECVVV
                     ATGKIDIITNGKSTYKINNGTDKLKGITGTGCMTASLIASFMAVTENILEAATMGVLT
                     MSLSGELANLNNPPIGTFKENLMNAIYQMDIDTLSKNSNIEFLN"
     gene            27423..28055
                     /gene="thiE"
                     /locus_tag="KRL67_000935"
     CDS             27423..28055
                     /gene="thiE"
                     /locus_tag="KRL67_000935"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HBH1521840.1"
                     /translation="MIDKEFLKKCLKLYLVTDSEMLKGRDFYKCLEDAISSGITTVQL
                     REKNASGREFLRKAMKLREITKRYGVKFIINDRVDIALICDADGVHVGQSDIEVREVR
                     KLIGNNKILGVSARTLEEAICAKNDGADYLGIGSIFTTSTKLDAKSASFETVKEIKEK
                     VDIPFVLIGGINLDNIDKLKCLESDGYAIISAILKAEDISKEVEKWTLKI"
     gene            28194..28496
                     /locus_tag="KRL67_000936"
     CDS             28194..28496
                     /locus_tag="KRL67_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBH1521841.1"
                     /translation="MIRILLVCVGGMSSTLLVNKMEKDAKKRNIDCKIWAVGEGNIKS
                     ELDNFDILLLGPQLRFMLDDVKSIVGDRAPVAIIDMVNYGTCNGHAVLNSVLEILK"
     gene            complement(28562..29311)
                     /locus_tag="KRL67_000937"
     CDS             complement(28562..29311)
                     /locus_tag="KRL67_000937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBH1521842.1"
                     /translation="MLNILWRSMKWRFKNPISFVVTILQPFLWLVLYSSIANQTMNNI
                     NINNYTAFILPGIIVLVVFSSCSSGGIINFIMKNSGSFYRVLIAPISRYSIVLGQLLE
                     AILVSFIEVAILCIVSIFFSVRIESGIGGILLMIVLIFMTAFFLSSLAYSISLLLPNE
                     IVYETIMTAIVLPIFFLSSALFPIENLSGGLKVAVMLNPFTHVINALRSLIFGETILI
                     GDILPVILLFLILCCASFSLAMWRLKKEMVS"
     gene            complement(29305..30267)
                     /locus_tag="KRL67_000938"
     CDS             complement(29305..30267)
                     /locus_tag="KRL67_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBH1521843.1"
                     /translation="MLAIEIDNLVKEYKNGVKALNGLSFNVNSGEIFSLLGPNGAGKS
                     SLINILTTFYKPTSGNVTMFGKDLVDNPSWIRTQIACVAQQISIDEHLSLMENMVFQS
                     KMYKVEPQIAKQRIDSLIDKFDLSSYLKYPTSSYSGGVKRRLDIAMNMVSSPKILFLD
                     EPTVGMDVDSRKSMWDMLLKIRDEYGTTIFLTTHYLEEAEQLSDNICIMKNGKDLAQG
                     TPSSLRSYIRQNILRITFHNTEDIKKYKDAIKSTGLVKFMNVRENSIFISVNDSRTAF
                     TLINKWLLEHDIKFDAIEIVEPSLEDVFLALTSSKKDLKEGWEC"
     gene            complement(30271..30729)
                     /locus_tag="KRL67_000939"
     CDS             complement(30271..30729)
                     /locus_tag="KRL67_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HBH1521844.1"
                     /translation="MSYKIEIKNIESVTVATMRYNGPMTEATKYFPNVFKAIKGKSNG
                     APFFCYYDVDQKTGVAEMELCVPTAETPNRMGITTKEFPKTKALFFTHIGSYSNLPKT
                     YEMIFKYIHENNIKIQTPWREVFIKGPGMLIKGNPDNYITEIIFPLKEEE"
     gene            31085..31441
                     /locus_tag="KRL67_000940"
     CDS             31085..31441
                     /locus_tag="KRL67_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBH1521845.1"
                     /translation="MNFELDNTTPIYLQIVKYIKRQIVIGELKPGETIPSRREMALNL
                     KVNLNTVQRAYKEMGDMNIINTFKNYQSSVTVDENILKNLKLELINESLSVFIEDMKA
                     INVSKEEVLKIIEDKY"
     gene            31512..32228
                     /locus_tag="KRL67_000941"
     CDS             31512..32228
                     /locus_tag="KRL67_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBH1521846.1"
                     /translation="MIEIKNVSKTYKRMQGLKIRKIKALKNVSFNIEKGKITALLGIN
                     GVGKSTMLKAIAGLINIDSGEIRIDGEKINEKVYNKLAFVPDVQSHFSNTTIKETFEF
                     MEIFYSKWNKEKSKEMMDIFKLDEDEIIDNLSKGNIARVKLILGFCQDPEYILLDEPF
                     TGIDLFKREEFIGVIAQYMEENQAIIITTHEIVEIESLVDEVVILDEGQIITSFNAEE
                     LREREGKSILDKMREVYKNE"
     gene            32221..>32849
                     /locus_tag="KRL67_000942"
     CDS             32221..>32849
                     /locus_tag="KRL67_000942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBH1521847.1"
                     /translation="MNKLLYLINVDLRRSNKFYIAYISFFSLITLSLNLFEVNKFKNS
                     KFIMNLAIGDFGGIFYGIGILSNLGFIQSILFFGMLGLFIYYIFMWKREFFSNNQSIY
                     TLMMLPQNKFKICISKSIALITMIYGFLISQIATLFICKYIFNFIFRNMPIINMNFAK
                     DLYYLNLKSIPIDFISFMAIYVFLLLIAISIVNCCILFIFSYINKIYVI"
BASE COUNT        12653 a         3593 c         5787 g        10816 t
ORIGIN      
        1 tgtcatataa ttacttaaga agtattatta taaataacat tgaaaggtag tggttagaat
       61 atggataccc ataataaata tgtaaatttt ataaaaaata tacctgtacc ttttttatat
      121 tgtagaattg taaagagaca agaagatata gaatatcgag tagaatatat aagtaagggt
      181 atgggcaaag tattacaatt agaagaaggt atttgtgata aaaatatact agatgtattg
      241 cctgtattta agtctaaaaa atatttcaaa gagttgttct caaatgaagt ggattgtata
      301 aaaaggtaca ttccgacact taaaaactgg ataaatataa aaaaacagat aataggggat
      361 tcatacataa ttttgtattt tggtaaaatt gtatttgatt atagacagat aatcgattcc
      421 tttgataaaa aggaaaaagt agcttatatt aaagacgaag aaggcatata tatagattgc
      481 agtgaaaatt taataccaat attaaataac aacattaaaa caacgaaaga tatctttggt
      541 aaaaatgata tagaagtatg gggagagaat acaggaaaat tatttagaga tgattataga
      601 gaaggagtat ctagtaaaaa aagatttcta caaaatcttt ttgaatatga agaaacattt
      661 tttatggttg aaaaatattt tttgtatgat gaagacgaac ttttaggtac tataggtata
      721 atagacaata taatatatag tgattacagt aatagaaact ataactcaaa agatttaatg
      781 aagatgatag agcactcaat tccagagaat atgttttata aagatgtgta tggaaattat
      841 attggattta attcaggttt tttgaattta gcttgtatga ataaagaaga attattaggg
      901 aagaatagtt ataaaatatc agaagaagaa gttttaatag ataagatatt tgaaagtgat
      961 aagggtgtaa ttgaaaataa aaaagtagtt acatttgagt taaatgtgag tataaatgat
     1021 gaaaataagt gtattgagat tacaaaaagg ccgttttttg atagctatgg aagtgttatt
     1081 ggaataattg gaacagcaag ggatataagt agaagaaaga gattagaaga ggaaatggat
     1141 aaaactagga tggaattttt tgcaaattta tcccatgaac ttagaacacc tataaattta
     1201 atatcatctt cattacaagt gatagaaaaa aaagaagcag atttaataga atcaaatgat
     1261 actttaaaaa gaaatttagg tataataaaa caaaatggaa atagaatact gaggttagta
     1321 aataatgtta tagactttac aaaaatgcaa tcaggatatt tagattttaa accagaagaa
     1381 tctgatataa ttgcatttat agaggaaata tgtatgtctg tagctgattt cgcaagtcaa
     1441 aataatatac aacttacatt tgatacagaa atagaagaat tttcaatgtt atttgattca
     1501 gaaaaattgg aaagaataat attaaattta ctttctaacg gaattaagta caataaggaa
     1561 gatggaaaaa taaacatatt tttatatgtg aaagataata tatttaatat gaaaatatct
     1621 gatagtggta taggtatacc aaaagaaaaa atagataaaa tatttaatag gtttgaacag
     1681 attgacaatg aactttctta cagagttaaa ggaagtggca ttggactttc attagtgaaa
     1741 tctttggttg agttgcatga gggaagtata tctttaaaaa gtcaacttgg tattgggagt
     1801 gaatttatag tttcattacc tgttagaagt aaaaataata ttgaaaaata taatcataaa
     1861 agagaaatca gtaatgaact aagtaaaaaa ttagaaatag aattttcgga tttataagtt
     1921 taagtataaa aataaaattt taaaaaagta ttgacaattg atttagaagg gtttaaaata
     1981 aattacaaca caataaataa aaaggcaatg aataggaaga gtaaatctaa ttcgattacc
     2041 agagagaaga gtccattggc tggaagactt tttaaattag agggattgaa aactacctag
     2101 gagctacttt ctgaagtcat ctgcatcagt agattttagt aagaatagtc gttagctgcg
     2161 ttaaagtgta aagtgagttt tcatgatata tgaaaactaa attaggtgga accgcgggaa
     2221 taatactctc gtccttatgt tataaggatg agagtttttt gttatttaaa aatataaaca
     2281 gtaatcataa taattataga aaatataaaa gcgatgaata ggaagagtaa atttaattcg
     2341 actatcagag aggaaagttc acaggctgaa agactttcta agtttgagaa attgaaaact
     2401 acctaggagc tgtggtctga agttacttta aaaagtttta gtaagactaa ccgttaactg
     2461 cgttaaagtg taaagtggat tttatcaata aatgataaaa tcaaattggg tggaaccgcg
     2521 agtatagact cgtcccttta tatagggatg agtctatttt tattgcaata aaagattgaa
     2581 aataaaaaaa aatcggaaaa ttataaattt aggaggaaaa taaaatggag aataaagtga
     2641 aaattggagt actaggatat ggagttgtag gaagtggact tatagatata atagataata
     2701 ataaagaaaa aagaaatata gaaatagttg gaattttagt taataatttg gaaaaacata
     2761 aggataaaaa atattctaat ataataacta ataatataga tgatatattt aataaagata
     2821 tagatatact tgttgaagtc atgggtggtt tagaaccatc attatcttat ataaaaaaag
     2881 cattaaacaa taaaattcat gttgttacag caaataaaga tttgttagct gaatgtggag
     2941 atgagcttgc aaaattagca agtgaaaata aagtaagtat taaatttgaa gcttcagttg
     3001 caggaggtat accagtatta aaaccaataa tagaatcact agaaggaaat aatatagata
     3061 gtataaatgc aattttaaat ggaactacta attttatatt atctaaaatg tatgatgaga
     3121 atctatctta tgatatggca ttaagacaag ctcaagaatt aggttttgct gaagcaaatc
     3181 cagagtcaga tgtattaggg tatgatgctg ctagaaaact atcaatatta tcaactctag
     3241 cttatgacaa tagagtatat tggaaagatg tgtatttaga aggaattaca gatatagatg
     3301 aaaaagatat tgaatacgca aaaaaattaa attgtaaaat aaaattgatt ggtcaaagta
     3361 agtatgaaaa tgataaagta agtgcttttg ttagacctgt tttagtagaa aaagataata
     3421 tccttgctag aatagacaat gagttcaatg ctgtaatagt gaatggagat tcagtaggag
     3481 aagtttcttt tgtaggtaaa ggagctggaa gtttagcaac aggaagtgcg gtttattcag
     3541 atgttataga tataatagat aatagagttt caagtataga ttcttttact aaagataaaa
     3601 tacaagttaa taaaatagtt agagaaaagt gtggagcact tcttagattt aaaaaatgta
     3661 ataaagatga aattctgaat atagttgaga attgtttagt aaaattcgat attttaaatg
     3721 atgatgatga acttgctatt atggtttatg ctgattctga atatgaaata aacaacagtt
     3781 tatgtctaat aaaagataaa ggatattgcg aaaaaatgaa taaaatgttg aaaataagtt
     3841 aaaaaagaaa aaatataagt ttatatgtaa catattttga cgattttaat actatatagt
     3901 atgagatgaa acaaaacatc tcataaaaaa attaaaatta aagggagaga tatgttatat
     3961 ggaaaataaa aaatgttatt cagaagattg gtatgaaaga ggagaatcta cagctaaatg
     4021 gttccaaaat gatagagaag aatatgaaag agcagcatac gatgaagata gagaaagaag
     4081 aggttcaaac tgtggttgtt cagattcagg agaaaataga cctagaaatt gtgaaagatt
     4141 tagaagagaa gctgagataa gagaaagaga agcaagagaa gcattctgtg aatcttcaga
     4201 gaaaaagaaa gaagcattag catatgaatg tgaagctaga aaattatggg aagaagcaga
     4261 aaaatactgg gatgaatatt caaaatacaa ctatcaagga attgaatatt tagcagaagc
     4321 tgctagatta tttgatgaag gtatggaatg tgaagctaga agaaatggaa ataatggagg
     4381 aaacaataat aattgttgcc ataaatgcca taaatgtaat tgtaactgct gtagaaaata
     4441 aaaaaattca ttgacaaatc aatagttaaa atatatcata ttatgtataa taaagagaaa
     4501 attcagaaaa aaataatgat tagaagaaat ataagtatat ttattagaca aatagtgctc
     4561 agttagtgag gtgagtacaa tatgattaaa ctcccatcct ctagcttttg atagagggtg
     4621 ggagttttta tttattgata tatcttaaat gaggataata ttgtaaaata aatgagtatg
     4681 atattgtaaa attaggaggt aaaaaatgaa gagtattaaa acatcaagta tgaaagaaaa
     4741 ggtagatgaa aagatattaa gagagcaagt aagttctata atagaagata taagaaataa
     4801 taaggatgtt gcacttaaaa aatataatga gaagtttgat agaaatacaa gagatgaatt
     4861 tagaattact aaggaagaaa taaaggaagc ttataagcat gtagatgacg aatttataaa
     4921 taacttaaag attgctgcca aaaatataag agaatttgct aaagctcaaa aactaagttt
     4981 tgaaaaccct tttgaaaaag aaatttatcc aggtgtaatc ttaggtcaaa ctaatattcc
     5041 aatagagtca tgtcttgcat atgttccagg aggtcagtat ccattgtttt caacagctct
     5101 tatgcttata atacctgcaa aagtagcagg agtaaaaaga atagtggcat gttcaccaac
     5161 tatgaaaaat actgaaaaga taaatcctaa aacattggta gctatggata tagcaggtgc
     5221 tgatgaaata tatgcaactg gaggagttca agctatagca gcatttacat atggaacaga
     5281 aaagattaat ccagttgaca taattgtagg accaggaaat aagtttgtaa ctgaagctaa
     5341 aagacaatgt tatggacaag taggaattga ttttgtcgct ggaccaagtg aagttcttat
     5401 aatagctgat gaaacttcaa atccagttta tattgcagct gatttattag cacaatgtga
     5461 gcatgattta aatgcaagag gaatattatt gacaaatagc ttagaaatag cacaagaagt
     5521 tgaaaaaaat atagaaagta tgttaaaaga tttgccaaca aaggacatag catatagttc
     5581 atgggaaaat aatggagaga taatactggt tgatgatatg gaagaagcca ttaaaataag
     5641 taatttttat gcaccagaac atttagaagt tgcagtaaat gagtataatg atatttgtga
     5701 tagattaact aactatggtt ctttatttat tggaaatctt tctgctgagg tatttggtga
     5761 ctatgtctca ggtaccaatc atacgttacc aacacttaaa gcatcaagat atactggtgg
     5821 tgtctgggtt ggaactttta tcaaaacttg tacaaagcag atatttaatg aaaaagctat
     5881 acaatcatta gcacctgtag ctgaaaagct ggcaaaagaa gagggattat atgcacatgc
     5941 aaaggcagct gaggttaggt ttaaaaagta gtaaaaaaat tctatgttgt cagtcgagat
     6001 ttagtattag actgagataa ggaaaacgcc gagaacctga ataaatacta tattttgtgg
     6061 gtcatcggcg tttcttttat atctgtatat gataggataa tactacctgt gtgtttaaat
     6121 gctaacttaa tatttctttt ctaaataatg tttatgaaat agttaatatt tttagttaat
     6181 acttgaaaaa agcttttgct aactgtgcaa attcgtctgc atttgtcaat atagcagggt
     6241 gtcctccctg actaaaaata tgaatacttg cttttgaaat aaaagttgcc atttgtttgt
     6301 actcttcttc aagattatga cgacacattt catcttcctt acttcctgta aataaaattg
     6361 gcatttctaa tttacacaat tctttatgaa acaaaggtcg tttctcgttt gcacattgca
     6421 atagagcttt tgtatcaaga ttaacaacat tttcccaatc ttttccctga caccattcat
     6481 agaattttcg tgcttgaata tcttgctttg atttttccct tccagtgatt agattatcta
     6541 tgaagttatc attaagagta cgtccatcaa agctatctgc tataattgct tcaactaaat
     6601 cgggacgttc taacccagta ttaacagctg cccatgctcc tccacttgtt ccaattaagc
     6661 caactttacc acaattcaaa tgttcaatca gtgctatcgt ctgtaaggct tcatcatgcc
     6721 acatgtcagg agaaaattga gaaactctat ccgactgtcc attacctaaa aaatcaatta
     6781 agatacatct aaagttttct acatataatg gcataagtgc tttaaacatc ttagaagaac
     6841 cagtattacc atgcaggaaa attatcggtt taccttgtcc atattcttta taaaaacaag
     6901 aattaccttg atactgaaaa tatgacatat attctcctcc tttttatttg ttaagttaat
     6961 tataacaata taggtatgaa tgaaaaacct catgttcttt cttagaataa aaatgtgcca
     7021 cgcataaaac gtgacacatt tctaattata aattttgctg tttaagtatt ctttgtatgc
     7081 ttttctctgt caaaaagtat ttctctgata actgctttat actcattcca ttttggaagt
     7141 ttatacagat ttgagaatta cgagaagcta agacttgttt ggtgtttgtg ttttcacccc
     7201 aatgattttt gtgctcttgc tttttgggaa tataaatcac tttaccatct atatattctt
     7261 gcacttgctc aatcagttct ttaggcagta agtcaattga attttgatag ctcattttgc
     7321 acctcctaaa taattattct ttaggattag caaaggctac tgccactcac aattaaaaat
     7381 aagatttttt attatgtgca atagcctttg ctatgcacaa gttccaattc ccataaactc
     7441 aatactcctt tctggtttga aaaattcatc tgaacattga tttcgttttt aatatagagt
     7501 tcaatgacaa atttacattt ttattatata tattcaggta gataattgca actatctttg
     7561 tatgagggta ttttattata gtcaatacta taatatgtat ttatatcatc aaactaatat
     7621 gaacagttga aaggaatatg aacaataaaa cttaatcagg aagaataatt atgaagcaga
     7681 gaatatagaa atttgatttt aaagttttat tgaaaattta ttgacaaatt taacttgtaa
     7741 atattatact aaacttaata aataaagttt ataaagtaag gtgttgtcta tgaaaaatat
     7801 agttggaaaa ccgcaaacga taaaaaaagt aaatgaagat ttaataaaaa atattataaa
     7861 aaatgagggt cctataacaa aaccagaaat ttcaagcatt actgaattga gtttagctac
     7921 agttaataag attgtagagc agttagcttt aaaaaatgag gttaaagtaa gtggactaag
     7981 tgaatctact ggaggaagaa gagcgcaatt atttgaaata aatgcaaact tagaacacat
     8041 aatagcttta tattattata gaaattgttg tataggggta gttgccaatt tattaggtga
     8101 aataatttat caagaagaat ttaatataag aatggataca tatgaaaacg ttaaccaaga
     8161 tacttttttt gttatagata ctttaataaa aaaggaaaaa ggaaacaata taagggctat
     8221 tggaattggt gttccaggag ttgtaaaaaa aggtgtagtt aaaaatatac caactataaa
     8281 aagttgggaa ggtataaatt tagaaaattt aataaagagt agatacaata ctaaagtgtt
     8341 tatagaaaat gatacaaata ttactactat tgggatttat caaaaacagt ataaagacaa
     8401 atataaaaat atggccctta tgtatttaga agatggtatt ggttcaggta tagttataaa
     8461 tggtgagtta tatttaggtt ctacaaattt tgctggagaa ttaagttatc taaatataga
     8521 atttggtaag tataactcta ataacttaaa tcttgaagat tatattatga gtttaagaaa
     8581 agattattta gaaagtaaag ataataaatt taagaaagaa attttatctg taatatcaaa
     8641 ctttataagg actcttgtat gtgtcctaaa tccacaagct atgattatac agtgtagtat
     8701 attaaccaag agtgacatgg agtttataga aaaagatgta agcaatagtg ttggagaaga
     8761 aaattttcct aaattaataa aagtagattc attgagagaa ggcagtatac atggagtaat
     8821 tagtatgtgt ttaaaagaaa ctgactatca atattcctta tctaataaaa aaggaggata
     8881 ttaatgtgaa atatttggtt tcaatagatg gaggaggaac aaaaactaaa ttttgtgtaa
     8941 gtgacttaga tggaaacata cttaaagaac ataccactgg ttcaactaat tataagtcag
     9001 ttggaattaa acaaacatat gaaaatatta ataatggatt taaaaaaata ttaaaagatt
     9061 tatatatcga ttatgacgat attgagtaca ctgtatttgg tatatctggt tgtgattctc
     9121 ctaatgatta taagattata atggatgaaa tattaaaaat tgggattaac aaagaaaaaa
     9181 tatatctggc aaatgatgca gttttagctt tttatgcaca agctgattcc cctggtttag
     9241 taattgttgc tgggacaggt tcaattatat taggaattaa agaagatgga gaaatatata
     9301 gagttggtgg atgggggtat aattttagtg atttgggttc tggatatgat atagggagaa
     9361 aattattaaa gaaagtatta ctttattgtg atgaatgtca tgagtatagt gatttattta
     9421 attgtgtctt agatttcttt ggagcaaata gttttgaaca acttgcatat atgattactg
     9481 atataaacaa taatgttgaa atagcaaact tagcctcatt agtaattgat tgtgccaaac
     9541 aaggtgataa attggctatt gaaatattaa gagaatcatc aactgaactg tctaaattag
     9601 ctcaagtaat attgagtaaa atatctaatc caatgaaaag ggataaaagc gaaataaata
     9661 tagttttgtc tggtggcaca ttaaataaaa ctgtatatgc tgaaatgtta atgaataatt
     9721 taaaaataga aaataaagat aaaaatttaa aatttatatc tcaagaaaat cagccagtat
     9781 atggggggat aagacttgct atggcattag ccaataggag ggtcaagcat ggatagaaaa
     9841 aaggtaatag tacaaatttt tactggaggt tttaagacta aagaaataaa gtttaatgat
     9901 ataaaatgta agctactaga attactggac tgtattgata tagaaaaggt tatcatgggc
     9961 tggagtgtag atgaagaatt atatgtagaa actaaaaaac tattaaataa atataatgtt
    10021 gaactatatc tttggttgcc atgtttttca gaaataggat tacttgaaaa aagtaattta
    10081 ctaattgatt atacggggaa ggaagtcaag ggctatgctt tacaagagga tgaaaatttt
    10141 gaattttatt gcccaaataa taaggaaaat ataaaaaata taaagaaaat atatagtact
    10201 tattttagtg atattaaatt tgatggaatt tttttagata aaataaggta tggttctttt
    10261 tcgaataaac taaatggtgt ttttaattgt ttttgtaaag attgtttaaa tatatatgaa
    10321 aaaaataata ttgataaaaa gtcattaatg atagaaatga ataatatgtc taagggagtt
    10381 acagataatg aagatactcc atttggtata acaaaataca ttaatggaaa atacaaattt
    10441 aaaaataaac tatgggaaga tttttttgaa acaaaaaatc aaaatatata caaggcactg
    10501 ttagagataa gtagttattt taaaagtaaa ggattgaaaa taggtataga tactttttct
    10561 ccttttattt cttactttac aggtcaagat ttagaaaaat tacaagaagt agcagatttt
    10621 ataaaaccaa tgatgtatag aactacaaaa gctccagctg gtttgccatt tgaatttgaa
    10681 aggtttattg atgaatccat atataagaat aaagagattg caaaaagaaa tttcttagag
    10741 attttaggaa ttaaagagta tgaaggagat gtatatccta tagaatttgt actagaggaa
    10801 atcgatttta tgacaagaag tcttaataaa gatatatatg taggagttga aataaataaa
    10861 aaggaaatta tagctcctgt tacacctaaa tacataagtg aaaatatttc aaatataaat
    10921 aagacaacag taaaaggata tgttttatct tgggatttgt tatcagcacc aaaagaaaat
    10981 attgatgaaa ttataaaatg gtttcggaat tagtgaggtg atatagtgag taagatattg
    11041 gaaatgcaac aaatatataa aagttttgga aaggtagatg ttttaaaagg gataaatttt
    11101 gagctagaaa agggtgaagt aagagctctt ttgggagcta atggagcagg aaaatctaca
    11161 cttattaaaa ttttaggtgg agtacattct caaactaaag gtaaagtgat tttggctggt
    11221 aatgaaataa tgtttaaaaa tccacatcat tctaaagaaa atgggattag tataatttat
    11281 caagaattaa gcttgatacc aacacttaca gtacttgaaa atgtttttct tggtagagaa
    11341 cttttaaaag gaagtgtgtt tttaaacaag aaagacatgc ttgaagaata tatgaaaatt
    11401 tgtgaggatt ttgattttaa tatagaccca catataatag tttctaattt aagtgtagca
    11461 aaccaacaaa tggttgagat aatgaaagct gtttcatgta attctgaaat tatcataatg
    11521 gatgaaccta caacatcact tactaataat gaaaaagagg ggctatttaa aataataagt
    11581 agactaaagt ctaaaggtaa aacaatagtc tatatttctc acatattaga ggaagttttt
    11641 aaagtatgtg atagtgcaag tataatgaaa aatggaatta tgattggaac ttatgatgta
    11701 gaggatttaa ctaaattaaa gataactcaa cttatgacag gaaacgataa tcacaaactt
    11761 gttgaaaaga aggattatac atttgcaaag tatgaagagc ttccagtact agaagtaaag
    11821 aatataagct ggtctaacaa aataaaaaac gtttcattta aagtttatag aggagaagtt
    11881 gtaggtcttg caggtcttgt aggttcaaga agaactgaaa ttataaattt gatatttggt
    11941 atagataata aagacagtgg agacattttt atcaatggta aaaaagtaga cataacttct
    12001 cctaaggtgg ctataaaaaa taaaatagga ttgattcctg aagatagaaa aaatctagga
    12061 ttaattttag aacaagctat atacacaaac tcatcatcag tacaaattga taaatttaag
    12121 aaattcttgt ttatagatag aaataaagaa gttgagtttt ctaaaaaagg cgttaaggaa
    12181 ttaggaataa aagttagtga tgtaaagcaa gaagtaaaag agcttagtgg tggaaatcaa
    12241 caaaaagtag tagtttctaa gtggttagag aaagatttag atttaataat atatgatgag
    12301 ccgacaaagg gaatagatat tgctgcgaaa gaagatatct tcaatacaat caaagaattt
    12361 tcaagtaaag gaataggagt aatattcata tcttctgatt tagaagaagt aataagagta
    12421 agtgatagaa ttttagtaac tagagaaggt tctattgtag atgatttaaa aaatgagaat
    12481 atacaggttc aagacataat gaacagtata tttaatgtat aaaaaatgaa aaatggagga
    12541 ctgagatgaa gaaagctttt gagaagttaa atttaagaga ttatggagta ttactaggtt
    12601 ttttagcgtt atgtatagct ataagtattg caactccatc atttttaggt aaacaaaata
    12661 tacttaatct tttacgacaa tcttctataa ttggaataat ttcagcaggt atgacttttg
    12721 ttataatatc tggaaacttt gatatatcag ttggagcagt atcagctctt gcaggtgcta
    12781 taacaatgaa atttgcgact ggtggaacta atttgtttgc atccatgctc ttaggtatag
    12841 caatttgtgc agtaatagga ttaatcaatg gtatagtagt tgcaaagata aatgtaccat
    12901 cattaatagc tactatggct atggtcacta ttgtaagagg attgctgttg atgcttacag
    12961 gcggatatcc aattactgaa aatataccaa tacttgatta cattggaaat ggatatttgt
    13021 ttaatatacc aataccagta gtaattttct ttatagtggt gttagcttcg tttattgtac
    13081 ttaataaaac taagtttggt agatatgttt actctgtagg agggaatcaa gaagcaagta
    13141 aacttaatgg tataaatgta gattctcaca aagtgaaagt atttattatt aatgctgtac
    13201 ttgcaggaat agctggaatc gtactaacag gaagacttgg gactgcaact gcgattgcag
    13261 gtgaaggata tgatatggat gcaattgcat ctgtagtaat aggaggtaca tcagtggcag
    13321 gaggttctgg ttctgtactt aaaaccgtta taggagtatt acttatgagt gttataaaca
    13381 acagttttaa tcttcttgga atagatgtat acttccagta catatttaaa ggattaataa
    13441 ttttagcagc agttggattt gattcttata gcaagaaaaa gcttgcttca agataaaaaa
    13501 taagaggggg aattttttta tgaaaaagtt aatgaaacat ttagcattat taatatcatt
    13561 tattatgata tttagcttag ttggatgttc aaacaatggt agtaaaggaa aagagggaga
    13621 aaagaaaata gcagtattgc ttcctggttc aacaggttac tttgtagcaa ctaaacaagg
    13681 tatagatgca aaatctaaag aattaggtgt aagtgtggaa tatgcagatg ctcaatggga
    13741 tgctagtaaa caattatcac aagctgaaga ttttatggct aaaggggtag acatgattgc
    13801 attatgtgga gttgattctg ctgtaagtga gagaatagtt aaagctgcca atgattcaga
    13861 cgttcctata atagcattta caaattctat aggaagtaac ccaactggag aatttaaagg
    13921 tttggttaca tatataggac aaaatgagga agagacagga gcattaactg gaaaaattgc
    13981 taaaaactta ttaggagaag ctggtggaaa agctgtatta atagaaggag taccaggaac
    14041 tactccacaa gtattaagaa aaaaaggtct tgaaaaagaa ttaaaagata gtaatataga
    14101 aatagtttat aatcaaactt caaactggga gaaagagcaa gctttaaaag taacagaaga
    14161 tttaattcaa aagaaaactg attttaatgt tataatatgt caagatgaca actctgctac
    14221 aggtgcaggt caagctttaa aagatgctgg tattaaggat aaagttaaag ttataggttt
    14281 gggtggaagt aaagatggat taaaagctat aactgatggg ttaatagatg gtacaactta
    14341 tatgtctgct gtgaaagaag gtggattagt tatagaaaag gcatctaaat tccttaaagg
    14401 tgaaaaaata gaacctgtta cacaaattaa acaagttgaa gttaataagg ataatatttc
    14461 agagttcaag ggtgaatggt aaattgtcat agataataaa taagattttc agagttactt
    14521 tagaaactat ataaagtaac tcttttttaa tttaatgata aattattgaa tagtatttct
    14581 aaacaaataa aaaattaata taaacaaaga cttaattata ctaaattata agtcaaacta
    14641 atataaactt atggaaaaaa ggaaaacgtc taaagtattg gaattactgt agtgtaggta
    14701 tggtattaaa aaaacataaa aaaacataaa aaaattgttg aaaaaaatat agaaattttg
    14761 tataattaaa gatttgaaaa gtgtggtata caaagccaat tcttttgtat ataatatata
    14821 taaattcaga aaaaggaagg gatatgtatg gccaggcggt tatttttaga aaggattgga
    14881 cttatatttt taggttcagc aatattagca tttggagttt ataattttta ttatttaaac
    14941 aacataacag aaggtggagt gttaggtatt ttactactgt taaaaaattt attcaatata
    15001 caaccagcaa tagcaaatgt tgtaatagat ggtttactac ttttagtagg atataaattt
    15061 ttcggtaaaa aattccttat atattcaata gtagcttcta taactttttc agtgttatat
    15121 gacttatttg aagctatagg acctctagta ccacaatcac aaaatatgct tttatcaact
    15181 attttggcag gtgtaactgt tggaactggt gtaggtatag tagtaaaagc aggttgtgca
    15241 tcaggaggag acgatgcttt agcattagta atttctaaga ctacatcttt gaacataggg
    15301 caagtttatt tagcaacaga tgttattgta ttgttgttat cattatttta tctatcagcg
    15361 tttgatatct tttattcttt gattgctgtt acaattagtg gtaaggtaat tgattttatt
    15421 tattatcatg gtaaaagttt ggatatggat ataagtaatg atatagttcc agaatgttaa
    15481 tataaattaa ataactttag attttattag gggtggcaga tttgccacct tttttgtgtc
    15541 taaatcttta tagaaagtat aatcacatct aggtttttgc aaatctcaaa tatagaatta
    15601 tgttggaata actgggaata cgattgctag atgtgcgttt taaaaactca attttatatt
    15661 tatgaaatac taatcattaa tggatgtttc ttaatcctat ctttatacct aaaatgataa
    15721 gataaatgaa gtagcaatac aaaactaaaa tttgtaaaag agattaaagt aaatggagga
    15781 gctaaaatgt tggatatagt tatgatagca ttgttattca taggatttgt ttccatgaag
    15841 ttacttgctg attggtgtgg gaaacaaact gaaaaaaaat aatgtaatgt atggagggat
    15901 tacctatgtg gtttttggca gttgtaatta taatgttaat aatatatttg gtatatgcgc
    15961 ttattaatcc tgaaaaattt taaacagtat aaggctaata agtaagttga attgggggga
    16021 acaattttta tggtgcagat agtaatagtg ttagcgattt ttatgatttt agtaatacct
    16081 atgggcaagt atttatatca tatagctact aatcaaaaaa catttggaga tagactattt
    16141 gataaagtag acaattttat atataaggta tgtagtatag ataagaaaaa agaaatgaac
    16201 tggaaacagt atgccttagc tttgttattt acaaatgcag ttatggtatt tataggatat
    16261 atcatactta gaactcaaag tatgcatatt tttaatccaa gtggaattaa aagtatggaa
    16321 caaggactat catttaatac aataataagt tttatgacaa atacaaatct tcaacattat
    16381 tcaggtgaat caggactttc atactttagt cagatgacag tgataatata tatgatgttt
    16441 acatcagcag caacaggata tgcggctgct atggcatttg taagaggtct ggtcggtaaa
    16501 aagaaaacat tgggaaattt ttatgtagat ttaattagga taacaacaag agtacttttg
    16561 ccaggagctt taattattgg tcttatatta gttacacaag gagtaccaca aacttttgct
    16621 gggacagaaa cagtgactac tatagaagga aagcttcaag atatagctag aggtcctgtg
    16681 gcggctcttg aatcaataaa acatttagga acaaatggtg gtggattctt tggttcaaat
    16741 tcatcacacc catttgaaaa tccaacaata atatcaaata tagtagaaat attatctatg
    16801 atgatattac caggagcttg tgttgttgca tttggtcaca tgataaagaa taaaaaacaa
    16861 ggttgggttg tgtttggcgc aatgtcaatc atatttttaa taggtctagt agtttgtttt
    16921 aaagcagaaa gtgcgggaaa ccctatttta tcacaattag ggcttaatca aagtatggga
    16981 agtatggaag gtaaggaagt tagatttggt atagcacaat catcattatt cacaacagtg
    17041 actacttcct ttacgacagg tacagttaat aatatgcatg atacactgac tcctttagga
    17101 ggattagtac cacttttaaa tatgatgcta aacgtagtat ttggtggtaa aggcgtaggt
    17161 ctgatgaata tgttgatgta cgctataata gcagtattct tatgtggctt aatggtagga
    17221 agaacaccag agtttttaac taagaaaatc gaaggaaaag aaatgaagct tattgcttta
    17281 cttattatat tacatccact tttaatacta atgttctctg gtctttcagt agcaatacca
    17341 gcagggcttg aaggaatttc aaatcctgga ttccatggat tatcacaggt attgtatgag
    17401 tttgcatctt cagcagccaa taatggttca ggatttgaag gtctaggaga taacactatg
    17461 ttttggaata tgacaacagg tatagttatg ttctttggaa gatatgtgtc tattatagta
    17521 ctgctagcaa tatcaagttt gttagcatct aaaaaagctg taaatgaaag tataggaaca
    17581 cttagaacgg ataattttac atttacaata gttttagtac ttgttgtttt aattgttggt
    17641 gctttaacat ttttcccagc cttagcgctt ggacctattt cggagcatct agtgttgtgg
    17701 cactaaaaaa ttgggaggga gtaaagtttt atggaaacta aaaaaacaaa gtttattaca
    17761 aaagatattt tcaaaacttc cattatagaa gcttttaaaa aattaaatcc taaatatatg
    17821 atgaaaaatc cagttatgtt tgttgtagaa attggattta tagtaacatt gatacttaca
    17881 atagtaccta gtttgtttgg tgatgaaggc aataacttaa gagcttataa tgggattgta
    17941 acaataatct tatttataac agtccttttt gcaaattttg ctgaagctgt agctgaagga
    18001 agaggtaaag ctcaagcaga tacacttaag aaaactagaa aagatacagt agcaagggtt
    18061 atagatacgt taggaaatga aaaaatgata agtgcaagtg agctaaaact tggtgatatt
    18121 gttttagtaa atgctggaga agttatccca aatgatggag aagttataga gggtatagct
    18181 tctgtggatg agtcagcaat aactggagaa tctgcacctg tcatgagaga atcaggtgga
    18241 gatttcgcat ctgttacagg tggtactaca gttgtaagtg actggttaaa gataagaata
    18301 acaacaaaac caggagaatc gttcttagat aaaatgatag ctcttgtaga aggtgcttca
    18361 agacaaaaaa cgccaaatga gattgcttta aatactttgt tagtagggtt aacacttata
    18421 tttatggttg ttttaatatc tctttatcca atggcaaact atgcaggtgt aaagattcca
    18481 atttctacta tgatagctct tttggtatgt ttaataccaa ctactatagg tggattgttg
    18541 tcagctattg gtattgcagg tatggataga gtaactagat ttaatgtaat tgcaatgtca
    18601 ggaaaagcag tagaagcttg tggagacgtt gatactatga ttttggataa gacaggtacg
    18661 attacttatg ggaatagact tgcatcagaa tttatagcag ttggaaatgc agattcaaaa
    18721 gacttaataa attattctgt tatgtgttca ttaaaagatg atacaccaga agggaaatct
    18781 atagttgatt taggtatgaa attaggttca acaggtaaga caaaagaggc agaggaagct
    18841 gagtttgtag aattttctgc acaaactaga atgagtggta ttaacttagc tgatggtaga
    18901 gctataagaa aaggtgctta tgatagcata ataaaaagag taaaagaatc tggtggtaca
    18961 gtgccagaag atttagaaga aaatgtaaat cgagtagcta aactaggagg gacaccttta
    19021 gtagtgtgtg tagataatga gatatatgga attatctact taaaagatac agtaaaatca
    19081 gggcttgtag aaagatttgc tagacttcga gagataggaa taaagacaat aatgtgtaca
    19141 ggggataatc ccttgacagc agctacaatt gcaagagaag ctggagtaga tggatttatt
    19201 gcagaatgta aaccagaaga taaaatagat gcaataaaaa aagaacaatt agagggtaaa
    19261 atagttgcaa tgacaggtga tggaacaaat gatgctcctg cacttgctca ggcagatgta
    19321 ggtcttgcaa tgaatagtgg tacaacatct gcaaaagaag cagcaaatat ggtggattta
    19381 gattcagacc caactaagat attggaagtt gttgaaatag gaaaacagct tttaataact
    19441 cgtggggctt tgacaacatt tagtatagca aatgatgttg caaaatattt tgcgattata
    19501 ccagctatat ttactcttgc aatacctcaa atggagatat taaatgtaat gaaactttct
    19561 acatcattta gtgcgatatt atcagcttta atatttaatg ctataattat cccatgttta
    19621 atacctcttg caatgaaggg cgtaaaatat agacctatga aatcacaagc tatgttaatg
    19681 agaaatatgc ttatatttgg tcttggtgga gttatagtac catttttagg aataaaatta
    19741 attgatttat tgattacacc tttagtaatg gttcttaatt taggttaata aatggaggat
    19801 ataaaaatga aaacattaaa gccagcatta cttgtaagta ttgtattgtt agttgtttgt
    19861 ggacttgtat atccacttgt tttgacaagt gtaagtcaag ttgcatttaa agataaagct
    19921 aatggaagta tgatagaagt aaatggtgta aaagtaggtt cagaacttat tggtcagagt
    19981 tttacagatg cgagattttt taagggaaga gtatcatcag taaactataa tacttataca
    20041 aaagaagatt tagtaccaga taaagatgga aatacatctt atggaggtgt atcatcaggt
    20101 tcttttaatt atggagctac aaatccagaa cttcatgata gagtacaaaa agatataaaa
    20161 cagtttttaa aggataatcc aactgtcaaa aaagaggata taccaacaga tttgcttaca
    20221 gcatcaggtt cagggcttga ccctaatata agtccagctt ctgcaaaaat tcagatacct
    20281 gctgtatcaa aagcatcagg aatatcagaa agtaaattac aaaaaattgt agatgataat
    20341 acttccaaaa aactttttgg agttttaggt gaagatagag ttaatgtgct aaaggttaat
    20401 gtagaggtcg caaaaatatt aggtcttata tagtattttg tagaatttta gtaacaaaat
    20461 attacgcaag atttaattta aagattaaat tttaaagagt gaaaatcgat aatgattttc
    20521 acttttttat ttgaatatta ctaatatttg gaataaggtg attgtaagta taaaatccaa
    20581 aaaaaggttg cattatcaaa atgataatac tataatatat ttatcgttat caatttgata
    20641 atagatttgg acatatagga ggaaatagta tgttatataa taacgcatta gagttgatag
    20701 gaaacacacc tgtagtaagg ctgaataatt taggctgtac taatgggtgt tctaacatat
    20761 atgtaaaatt agaaaaagta aatccagcag gaagtataaa agatagagct gtttatgaaa
    20821 tgttagaggg cttagagaaa agaggagaat taaaaaaggg agatgtacta gttgaagcaa
    20881 ctagtggaaa cactggtatt gcattatcta tgataggaaa attaaaagga tatgaagtag
    20941 taatcgtaat gcccgaaacg atgagcgttg agagaagaga tttaatgaag gcttatgggg
    21001 caaatttggt gcttacagat ggtaaagctg gaatggcagg ttccatagaa aaagcaaatg
    21061 aacttttaaa agaaaatcca aactataaaa gcttaaaaca atttgaaaat aaagataatc
    21121 caaatgcaca ttataaaaca acagcagttg aaattatgaa tgatgtcaaa gatttagata
    21181 tattcgtatg tggagttgga acaggtggta ctttaatagg aactgcgaga tatttaaaag
    21241 aacaaaatcc aaatataaga gtaattgcac ttgaaccaaa aaaatctcca gctatatcag
    21301 aaaacaagac tggacctcat aaaatacaag gaataggtgc taactttgtt cctgaaaatt
    21361 atgattctag tgtagttgat gaagtaatat tagtagatga tgaagatgca tttgatacag
    21421 ttaaattact tgcagcaaaa gagggaattt tagtaggtat atcttctgga gcaaacatat
    21481 ttggtgctat agagatggca aaaaagtatc cagataaaaa gatagttaca gtagctccag
    21541 atggagttga taagtatatg tctatgggaa tattttaata attttatttg agggagtgaa
    21601 tactttgttt aagaaaataa ataaagatat tgaatatata atgaagaatg atccagcagc
    21661 taggtctaag atagaagttt ttttacttta tccatcagtt catgcaatga taatgcatag
    21721 aatggctcat gctctttata agaagaagaa gctttttaca gcaagattaa tatcccagat
    21781 atccagattt atgactggta tagaaataca tcctggtgct aaaatggggg aaggtatatt
    21841 gatagaccat ggaatgggtg ttgtaattgg agagacagct gaagtaggta atagggtgac
    21901 aatatatcaa ggagctactt taggagctac aggaaaagat actggaaaga gacatccaac
    21961 tgttggtgat gatgtattaa ttggtgctgg aacaaaaata ctaggacctt taaatatagg
    22021 ttcaaattca aaaattggtg caaattctgt tgtcgttaag gatgtaccta atggtgcaac
    22081 agttgttggt ataccagcaa aaatagtaaa aataagaaac ttagaacctg taaaaaagaa
    22141 taaaaaagaa gtaggctatg agtatgatga attagataat gtatattata tataacttta
    22201 gcataactat aaaataaact ttataaaaat tatttttaaa aggtattttt ttcaaatttt
    22261 aggacataat aaaaaagaac tgaatgttac agttttagtt catcaatctt tgataaacaa
    22321 gattttaaat ttgtagcgtc taggaggatt ttattatgaa agcaaatgtt aatcaagata
    22381 cttgtatagg ttgtggatta tgtccatcaa tatgtccaga agtatttgat atgaaggatg
    22441 atgggaaatc acatgttata gttgatgaag taccaagtga tgctgaagaa tcagcagcag
    22501 aagctagaga aagctgtcca gttgatgcaa tagatgtgca ttaataaaat aaatgcctat
    22561 aaaaattgaa ttttcaattt ttataggcat ttgtctttta ttcagttagt atttcaacac
    22621 ctgtttctgt aactaaaacc atacattccc attgagcaga taatttacca tcttttgtat
    22681 aaatagtcca attattttct tcatcaacga aaatttcgtg agttccttca tttaccatag
    22741 gttctattgt gaatatcatc ccaggaacca atagcatctc agtccctttt ttagatacat
    22801 aacttacaaa agggtcttca tgaaagctta atccaactcc atgaccacct atttctctaa
    22861 caacagagta tccatttttc tttgcataag agttgatagc atcacctata tctcctaaga
    22921 atccccatgg tttggcaact tcaagacctt tttggacaca ttcctttgta acttgaacta
    22981 atttttttgc attttcgtta acattaccta tcataaacat tcttgaagcg tctgaatagt
    23041 atccattaaa aatagtagaa acatctacat ttataatatc tccatctttt aatataatat
    23101 ctttacttgg aataccatga caaacttcat tatttactga tgtacataca cttttaggaa
    23161 atccattata gtctagtgga gcaggaatag ctccattttg tagtgtaaag tcatgaacca
    23221 acttatttat atcttctgta gacattccaa tttcgatttt atctgaaata taatccaaaa
    23281 tttttgtgtt tattacagca ctttttttta tttcctcaat ttgactttca ttttttataa
    23341 tatcatgagt tggtactata tgacctaact tagcaaatga atctattttg ttatccatat
    23401 ccatgtgaca ctttttatat tttttagtac ttccacacca acaactgtca tttctattta
    23461 acttcataac ttttcatcct tcttttatta tttatttaaa ttatgggcta cataaactat
    23521 tttaaaacat aaccattata aatattataa cataaatatt aagaaatcct ttatagatag
    23581 tgatttattc aaagtataaa ttattatatg gttattgttg atgaaaagag gatagttaaa
    23641 tgtattattg gatattagcg cacttaatag tttagttcaa gtttttcaag aaaatcatat
    23701 gtcagaggac aattttcctt taaagtattt tttgaatctt catcaagata ataatgtaca
    23761 aatgattctg caaaaaattc ttctataaac ttgcttatat atgcatgtgc aacttgttta
    23821 gggtcataaa gcttatatct ttcctgagca aatacttcta taaattcttc agaatttgat
    23881 tttttattta aaacgttttt atctatagca tgtgcagttt catgtaattc taaatttttt
    23941 gatgaatgta catttgcata gctagggtta ctataaccta ttttagcaac tatagaatta
    24001 tttctatata taccaggtac atctttccaa gtcttgcctg atcttaccca acatggaggt
    24061 aattgatatt ttaaatcact aaattctggt tcatcagtta gatttgaatt tattaatttt
    24121 atttttatat ccttttcttt aagtgtctgt aaatatttaa tatttatttt tgcaagtctg
    24181 cttttcatat tttcaacttc atctttatta taattcaagt catcaggaag tataagcatt
    24241 tgttcaatta tatctatgct actgtctttt atatcttcag attcttcatc aaatttatat
    24301 tgaacagtat cattttcata gatagttata tttgtattat tttccataga ataagagtga
    24361 atatcttcta aatctaaaat attcatttgt tgtaaaatat gttttgtttc gccactccat
    24421 actactaaag aaagtgaatt aaaattaatt agtggatttt cttttgcttt tatatcattg
    24481 cttatatcta catcaaatac atattcaatt gagtcctcta tgttaagact atctgtattc
    24541 tttttaatat ataaatacgc ttgttttaag tctatataaa tctcatctaa agatttgtca
    24601 caaaattctg ataaattatt agtaaagagt tcttcactta aaagttcatt tttatcagtt
    24661 atactttcaa ttaaaatatt aatttctttt atcatatcgt aaatacttat ttttctattt
    24721 gctttttcac tagatacatt gcatggcgca agagcaatgc aataaaaatc tttttctttt
    24781 ttataaaagg ctttaagtga ttttgataaa agtgtaaagt ctacacctat ctgtaagttg
    24841 accttaagtg agtcattttg tgataattca gtattatttc ttttagctgt atttaaattg
    24901 ttttccataa tcattgcccc ctttttatta gggtatcata atttttttat atgtgtcaat
    24961 ttattttttt gaaaattata atttttttga aaattttatt ttttataatt tgtgaatttt
    25021 ttattataga aataaagatg ttattattat gtcaaaattt agcattagat tcatgtagag
    25081 catgtttata catatattat aattaactct ttggatattg catattcaaa atacacacca
    25141 ttttttttaa ttctataata taatgtttat atatttaatg ttaggatgtg atttaagatg
    25201 tattttacat atattataag atgtaaagat gattctctat acactggata taccagtaat
    25261 atagttagaa gaatgaatga acataagtta ggaattaatt caaaatacac aagagcaaag
    25321 gggtttaaaa aactagaagt ttattttgta accaacacaa aaagcaatgc tatgaagtta
    25381 gaatactata taaaaaaatt aactagaaat aaaaaattgt ctataataaa aaatccaagt
    25441 atacttataa atttaattga taataaagag gattatatta ttggtaatga aatagaacaa
    25501 ttaacataaa tatttatagt aacattataa tgagttggta taatgtaaaa acttgacaat
    25561 aaatactatt gtaatataat gaagttataa atatagtaaa ttaaaatagc taggggagcc
    25621 aagagctgag aggtataccg acccttacac ctgatctgga taataccagc gtaggaaagc
    25681 ttagtaaaaa ttcaagtatg tattatttgt gatatatgct atttctaagc tatacctatt
    25741 gtaggtatag cttttttaat taagttattt aaataaaact ttaggaggca acaatgagta
    25801 attataaaat accaacactt acaatagctg gctctgattc atctggagga gctgggattc
    25861 aggctgattt gaaaacgttt agtgcaattg gtacttatgg tatgagtgtg ataacagctg
    25921 taacagcaca aaatactaaa ggagtttttg atgtagagga tttaaataag aaaataataa
    25981 aaaaacaaat agaggcagtc tttgaagata tacctccaag agctgtaaaa ataggaatgg
    26041 tttcaagccc tgagattata cttgaaatag ttgagaatct aaagaaatac aatccaaaat
    26101 atttggttgt ggacccagta atgatatcta aaagtggata ttatctatta aagccagagg
    26161 caaaagaaaa cttaattaag catttgatac cacttgcata cataattaca ccaaatatac
    26221 cagaggcaga agaaatcaca ggaataaaaa tacataatgt agatgatatg aagagagtag
    26281 gaaaagaaat actacaactt ggacctaaat ttgtacttat gaaaggaggt catttagatg
    26341 gagaagcagt ggatatttta gttggtaaaa atatatttaa agtttataaa agtgaaagaa
    26401 ttgataaaaa aaatacacat ggaacagggt gcacattatc ttctgcaata acatcttatc
    26461 tagcattagg atatgaaata acagaagcag taaatctaag taaaatttat ataacagaag
    26521 caataaaaag aagctttgat ataggtcatg gagtaggacc agtacatcat ttttataaat
    26581 ttgagtaata aaaaaatatt ggaggtagaa tatgtataat ttaattaaag atgtaaaaaa
    26641 attaaatcca ttggtaattc attatactaa taatgtaact ataaatgatt gtgctaatgt
    26701 aactttagct gtaggagcaa gtcctcttat gagtttttca tatgaggaag tagaagaaat
    26761 ggtaagtgta gcaaactctg tagtaataaa tataggaact atgaactcaa atatgttgga
    26821 tttattccta ttagctggaa aagcagcaaa taagtacaat aaacctgttg ttttggaccc
    26881 agttggagta tttgcaagca aagcgagagc agaacttaca agtagacttt taaatgaagt
    26941 caaatttagt gttgttaaag ggaatgtgtc agaaataaag tttataggtg gctttaatgt
    27001 aaggggtaaa ggtgtagatt catttgatga agaagaagat tctactgaga taataagaaa
    27061 gattgctgaa aagcttgaat gtgtagttgt agcaactgga aaaatagata taataactaa
    27121 tggtaaaagt acatataaaa taaacaatgg aactgataaa ttaaaaggaa ttacaggaac
    27181 tggatgtatg actgctagct tgattgcaag ttttatggct gttactgaga atatattaga
    27241 agcagcaaca atgggtgttc ttacaatgtc tttaagtgga gaattggcta atttaaataa
    27301 tccgccaata ggtacattta aagaaaatct tatgaatgct atttatcaaa tggatataga
    27361 tactttaagt aaaaattcta atattgaatt tttaaattaa taaagagtaa gggagactta
    27421 aaatgattga taaagaattt ttaaaaaaat gtttaaagtt gtatctagta actgactctg
    27481 aaatgcttaa aggtagagac ttttataaat gtcttgaaga tgcaatatct tctggaataa
    27541 caactgtaca acttagagaa aaaaatgctt ctggaagaga gtttttaaga aaagctatga
    27601 aacttagaga aataactaaa agatatggtg ttaagtttat aataaatgat agagtggata
    27661 tagcacttat ttgtgatgca gatggagtac acgtaggaca gagtgacatt gaggtgagag
    27721 aggttagaaa gcttatagga aacaataaaa ttctaggagt atcagcaaga actttagaag
    27781 aagctatttg tgcaaaaaat gatggcgcag actatttagg tatagggtct atattcacaa
    27841 catctactaa attagatgca aaatcagctt cgtttgaaac tgttaaagaa attaaagaaa
    27901 aagtagacat accatttgta ctaattggag gtataaattt agacaatata gataaactta
    27961 aatgtttaga aagtgacggg tatgctataa tatcagctat attaaaagct gaagatatct
    28021 ctaaagaagt ggaaaagtgg acattaaaaa tataaaattg gctctggtag tgaaaaaaat
    28081 ggaaaaattt gatataatct aagagtaaaa tttaagtaat tataaatgat aaacttttct
    28141 acatatactt tttaatcaaa atatatgtag atttggttaa aaaggagcta taaatgatta
    28201 ggatattgtt agtgtgtgtt ggaggcatgt cttctacctt attggtaaat aaaatggaga
    28261 aggatgcaaa aaaaagaaat atagactgta aaatatgggc agttggagaa ggaaatataa
    28321 aatcggagct tgataacttt gatattttac ttcttggacc acaacttaga tttatgcttg
    28381 atgatgtaaa gtctatagtt ggagatagag ctccagtagc tataatagat atggtaaact
    28441 atggtacttg taatggtcat gctgtgttaa attcggtttt agaaatatta aaataaatga
    28501 taaaagtgga ttctatataa tagagtccac ttttattatt tgttattaat aggtttaaaa
    28561 cttagcttac catttctttt tttaatctcc acatagcaag tgaaaaactt gcacagcaca
    28621 atattaaaaa tagtaggata acaggcagta tatctccaat aagaatagtc tcaccaaata
    28681 ttaagctacg taaagcgtta ataacatggg taaatggatt gagcatcact gctactttta
    28741 atcctccaga taaattttct atagggaaaa gagctgaact taaaaagaaa ataggaagta
    28801 caattgcagt cataattgtt tcataaacta tctcatttgg caataataaa cttatagaat
    28861 aagctagact tgataaaaag aaggctgtca taaatatcag cactatcata agtaggattc
    28921 caccaatacc agattctatc cttactgaaa agaatatact tactatacac aaaatagcta
    28981 cttctatgaa ggaaactaat atagcttcta ataattgacc aaggacgatt gaatatctac
    29041 tgataggtgc aattaagact ctgtagaaac ttccactgtt cttcattata aagtttataa
    29101 taccaccact actacaagaa gaaaacacaa caagtacaat aatacctggt agtataaaag
    29161 cagtataatt attgatattt atattattca tagtttggtt tgcaatagaa ctataaagca
    29221 caagccaaag aaaaggttgt aatatagtaa ctacaaatga gattggattt ttaaaacgcc
    29281 acttcatact acgccataag atatttaaca ttcccatccc tcctttaagt cttttttaga
    29341 acttgtaagt gctaaaaata catcttcgag acttggctct acaatttcta ttgcatcaaa
    29401 ttttatatca tgttctaaaa gccatttatt tattaaagta aaggcagttc tactatcgtt
    29461 tacacttata aaaattgaat tttctcgtac gttcataaac tttactaaac cagtactttt
    29521 aattgcatct ttatattttt ttatatcttc agtattgtgg aaagttatac gtagtatatt
    29581 ttgtctaata taactacgta aacttgatgg tgttccttgt gctaaatctt ttccattctt
    29641 cataatacaa atgttatcac ttaattgttc agcttcttca agataatgag tagttagaaa
    29701 tatagttgtg ccatactcat ctctaatttt taataacata tcccacatag attttcttga
    29761 atctacgtcc ataccaaccg ttggttcatc tagaaataga attttaggag atgataccat
    29821 attcatagct atgtccaatc tacgttttac cccacctgaa tatgatgaag taggatattt
    29881 taagtaacta gataagtcaa acttatcaat taaagagtct attctttgtt ttgcaatctg
    29941 tggttctact ttatacattt tactttgaaa taccatattt tccataagag ataagtgttc
    30001 atcaatagag atttgttgtg ctacacaagc aatctgagta cgtatccaag aaggattatc
    30061 tactaaatct tttccaaaca ttgttacatt tccagatgta ggcttgtaaa aagtagtcaa
    30121 aatattgata agagaagatt taccagcacc attaggacca agtaaagaaa aaatttctcc
    30181 agaatttaca ttaaagctta gaccatttaa agctttcaca ccatttttat attcttttac
    30241 taaattgtct atttcaatcg ctaacatata ttattcctcc tctttaagtg gaaatattat
    30301 ctctgtaata tagttgtctg ggtttccttt tatcaacata cctggtcctt taataaatac
    30361 ttctctccaa ggagtttgta tttttatatt attttcatga atatatttaa atatcatttc
    30421 atacgttttt ggcaaattac tataagaacc tatgtgagta aaaaataaag cttttgtttt
    30481 aggaaattct tttgtagtta tacccatacg atttggagtc tcagcagttg gaacacataa
    30541 ttccatttct gcaacaccag ttttttggtc aacatcatag tagcaaaaga atggtgcccc
    30601 gttagacttt ccttttatag ctttaaatac atttggaaaa tattttgttg cctcagtcat
    30661 aggtccatta taacgcatgg tagccactgt gactgattcg atatttttga tttcaatttt
    30721 ataactcatt tgtttacctc ctaatgtgtg ttcatattct tactagattt ttaagcatta
    30781 atacatataa gattttgatt agttgaaaac atataatcct atctcgtttg cttgttttca
    30841 agtctaacat aacataaaat atatatcctc tcatttgatg ctccattttg taaagttatt
    30901 atttaagctt tactttagat tttttagtaa attaaaataa agcaatttat aaaataagta
    30961 tttacaaatt aagtgtatta atcgtataat acacttaata ggatactaga taaggtctga
    31021 aataaaggcg tattttttta aagtaagtgt actatcgtat taatacacta gaataggagg
    31081 aatcatgaat tttgaattag ataatacaac tcccatttat ttgcaaatag taaagtatat
    31141 aaaaagacaa atagtaatag gagagttgaa accaggagag acaataccat caagaagaga
    31201 aatggcactt aatctaaaag taaatttaaa tacagttcaa agagcatata aggagatggg
    31261 tgatatgaat ataataaata cttttaaaaa ttatcaaagc agtgtaacag ttgatgaaaa
    31321 tatattaaaa aatttaaaat tagagcttat aaatgaatca ctatctgttt ttatagaaga
    31381 tatgaaagca ataaatgtta gcaaagaaga agttttgaaa ataatagaag ataagtatta
    31441 gtttttaaca aatagaaaac ttagtttaga aagtttattt ataagcatat tggatgtgga
    31501 ggatagtaaa tatgatagaa ataaagaatg tcagcaaaac atataaaagg atgcaagggc
    31561 ttaaaataag aaagataaaa gctttaaaaa atgtaagttt taatatagaa aagggaaaaa
    31621 taactgcatt acttggtata aatggtgttg gtaaatcaac tatgttaaaa gcaatagcag
    31681 gacttataaa cattgatagt ggagagattc gtatagatgg agaaaaaata aatgaaaagg
    31741 tatacaataa attggctttt gttccagatg tacaatctca ttttagcaat acaacaataa
    31801 aagaaacatt tgaatttatg gaaatttttt attcaaagtg gaataaagaa aaatcaaaag
    31861 aaatgatgga catatttaaa ttggatgaag atgaaataat tgataacttg tcaaaaggaa
    31921 acattgcaag agtaaaatta atacttggat tttgccaaga cccagagtac atacttcttg
    31981 atgaaccatt tacaggaatt gatttgttta agagggaaga gtttatagga gtaatagcgc
    32041 aatatatgga ggaaaatcaa gctattatca taactactca tgagatagta gagatagaaa
    32101 gtttagttga tgaggtcgta attcttgatg aagggcagat tataacttca tttaatgcag
    32161 aagaattaag agaaagagaa ggtaaatcta tattagataa gatgagggag gtatataaga
    32221 atgaataaac ttctatatct aataaatgta gatttgagaa gaagcaataa gttttatata
    32281 gcttatatat catttttttc attaattaca cttagtttaa atttattcga agttaataaa
    32341 tttaaaaatt ctaaatttat tatgaattta gcaataggag attttggagg tatattttat
    32401 ggaataggga tacttagtaa tcttggcttt atccaaagta tattgttctt tgggatgttg
    32461 ggacttttta tatactatat ttttatgtgg aaaagagagt tcttttcaaa taatcaaagt
    32521 atttatacac ttatgatgtt acctcaaaat aaatttaaga tttgtatatc taaatcaata
    32581 gctttgataa caatgatata tggttttttg ataagtcaga tagcaacatt atttatatgt
    32641 aaatatatat ttaatttcat ttttagaaat atgccaataa tcaatatgaa ctttgccaaa
    32701 gatttatatt atttaaattt gaagagtata ccaattgatt ttatctcttt tatggctatt
    32761 tatgtatttt tactacttat agctatttct attgtaaatt gctgtatatt atttatcttt
    32821 tcctatataa ataaaattta tgtaatttt
//
LOCUS       DAEPXF010000022        31956 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain HB27
            SAMN13376389-rid12479423.denovo.023, whole genome shotgun sequence.
ACCESSION   DAEPXF010000022 DAEPXF010000000
VERSION     DAEPXF010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13376389
            Sequence Read Archive: SRR10518432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 31956)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31956)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001069624.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001069624.1
            Genome Coverage       :: 315x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/15/2021 01:54:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,165
            CDSs (total)                      :: 4,076
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 89
            rRNAs                             :: 10, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 10 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 19 of 69
            Pseudo Genes (incomplete)         :: 44 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 10 of 69
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31956
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13376389-rid12479423.denovo.023"
                     /strain="HB27"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="China: Hebei"
                     /collection_date="2017"
     gene            <1..165
                     /locus_tag="KRL67_000943"
     CDS             <1..165
                     /locus_tag="KRL67_000943"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH1521848.1"
                     /translation="NKCVYIVTAYITSLKGHYSFVETQIDDFIKKYVEDIVDLCLHRI
                     MILLYECDLT"
     gene            complement(1033..1608)
                     /locus_tag="KRL67_000944"
     CDS             complement(1033..1608)
                     /locus_tag="KRL67_000944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GerMN domain-containing protein"
                     /protein_id="HBH1521849.1"
                     /translation="MKNKKIMLVLSILSISIFAVGCTNAQNGNDTSKKETKSNTNVEQ
                     PKEENNTEKEVPNNKAKPTPKEETKTQSATIYSFDVDKTDLIENKVDLSKIDEKTLFE
                     ELQKLKVVPESAKLNSFTTKDIDGVKTGILDVSSDFTKSNLGSDAETLMLDSVARTYI
                     KNMNVEQIKITVDGSNYESGHIVLEEGDYLK"
     gene            1868..3160
                     /locus_tag="KRL67_000945"
     CDS             1868..3160
                     /locus_tag="KRL67_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HBH1521850.1"
                     /translation="MEILSLGEKIKKLRKEKNMTLKELAGDRITAAQISHIERDKSHT
                     SYELLEYLGDKLDVSVEYLLETKEMQSKKITDNLILQSEIYIKENELNKAEDQIKEVL
                     DICKRYDLMENYGRCNFLMANINLKKSNYNDAIENFEKALYFFIKNSDSENILKCYLN
                     IGKIYMQEDFYKGAISHFNFAEEILSENKFEDVNIHKELYSKMAYCYIKLDNPNMSLK
                     YIEKINEIDNRNDRKEDVDILVLKANNMLEIGKYEESRDYFKKALKILESEDNKTGLA
                     NVYLTICDVYRKIGETDRVLEYSQKVYDIKKNDEDEYMMSGLFKIIEAYIDKEDYDLA
                     RKYCKIALASSIKNKNKFNEYKALRFYANMYKNQNEVTLAIEYLIKCIKIVSDLGNNR
                     ILANLYIDLGQLYSNISKEKELEYYQKGVFMYKNLEIM"
     gene            3265..3867
                     /gene="yedF"
                     /locus_tag="KRL67_000946"
     CDS             3265..3867
                     /gene="yedF"
                     /locus_tag="KRL67_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase-like selenium metabolism
                     protein YedF"
                     /protein_id="HBH1521851.1"
                     /translation="MNIKIDARGLACPKPVINTKRELDNLEEGVVVTIVDNETAKENI
                     LKLAKSLSCEANIVDEKENFISIEIRKGKNILDKQNVVDNKESELDNTCIFISSDKMG
                     LGNDELGKVLIKGFIYTLTESKPYPKYVLLVNGGVKLSAENEETIENLKILEKMGVEV
                     LSCGTCLDYYNLKDKLQVGTVTNMYTIVETLKNASNTISI"
     gene            3940..4203
                     /locus_tag="KRL67_000947"
     CDS             3940..4203
                     /locus_tag="KRL67_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3343 domain-containing protein"
                     /protein_id="HBH1521852.1"
                     /translation="MNQMYIVSFNSTHHAIRSEKLFGENSLKVMALPTPREITASCGI
                     SIKFSFEDMEKIKTILVKNNVDIKGIYCISKLENGSKEVEKLD"
     gene            4218..4403
                     /locus_tag="KRL67_000948"
     CDS             4218..4403
                     /locus_tag="KRL67_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 domain-containing protein"
                     /protein_id="HBH1521853.1"
                     /translation="MPMDLKIGDIVELKKQHACGCKEFEIIRTGMDVKIKCTKCSRLI
                     MLDRETLEKRVKKLIKK"
     gene            4506..5702
                     /locus_tag="KRL67_000949"
     CDS             4506..5702
                     /locus_tag="KRL67_000949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HBH1521854.1"
                     /translation="MAVKYAKRMQGLQGSEIRELLKLTQQPQIISFAGGMPAPELFPV
                     EEMKKVSVAVLEENGRSAMQYTTTEGYEPLREKIAARMNDKNKTNVNKDDILVTSGSQ
                     QGLDFAGKVFIDEGDVILCESPSYIGAINAFKSYQPKFIDVPTDSDGMIMEELEKILE
                     TTDRIKMIYVIPDFQNPTGRTWPLERRKKFMEIVNKFEIPVIEDNPYGDLRFEGETLP
                     SLKSMDTKGLVIFLGTFSKIFCPGYRLGWTCASPEILSKFNFAKQGADLQASTISQME
                     VSKFMDMYDLDAHVDKIKAVYVKRRDVMLKTMEEEFPEGLVFTHPEGGLFTWVELPSN
                     LNAKELMPKCLDKNVAYVAGGGFFPNGGRENTFRLNYSNMPEEKIIEGIKNIAAVLKE
                     AMGVEA"
     gene            5916..6194
                     /locus_tag="KRL67_000950"
     CDS             5916..6194
                     /locus_tag="KRL67_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HBH1521855.1"
                     /translation="MRNYELVYVVKPNSDEEVREAILNKVKEVVATDGEIVKVDTWGT
                     KKLAYPIAKFTEGFYVLVNFKSAVDVPKEIDRNLKINENVIRHMIVVA"
     gene            6231..6665
                     /gene="ssb"
                     /locus_tag="KRL67_000951"
     CDS             6231..6665
                     /gene="ssb"
                     /locus_tag="KRL67_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HBH1521856.1"
                     /translation="MNQVVLVGRLTRDPELRYIPGTGTPVASFTIAIDRDYVKKDGSK
                     ETDFIPVEVMGKSAEFCANYITKGRLVALQGSIRVDNYQTQSGEKRTFTKVSTRSVQA
                     LDSKNKSENSYRESAPAFEPSFEPQGLDPQGFQAIDDDDIPF"
     gene            6684..6911
                     /locus_tag="KRL67_000952"
     CDS             6684..6911
                     /locus_tag="KRL67_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HBH1521857.1"
                     /translation="MMNKKRRKKKRVCQFCADKNAKIDYKSTQRLQKYITERGKILPR
                     RISGTCAKHQRELTVAIKRARNIALLPYTLD"
     gene            7054..7374
                     /locus_tag="KRL67_000953"
     CDS             7054..7374
                     /locus_tag="KRL67_000953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG-like family protein"
                     /protein_id="HBH1521858.1"
                     /translation="MKIDKGSEITRNIKIIEWMKTEILMSVSDLFNLLFKGVKPLDEA
                     IQDTLANIIMITYLLAKRLGISFRDVDYKVKEKIKIGIDEDHSVERWYGDLSNLKKHM
                     DNRE"
     gene            7382..8338
                     /locus_tag="KRL67_000954"
     CDS             7382..8338
                     /locus_tag="KRL67_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YybS family protein"
                     /protein_id="HBH1521859.1"
                     /translation="MNNKIRLSKAMSIIVLAIIIALVIAYIPMLGIFSMLAAVPYVIV
                     GAITDRKYSLISILVTFCVLILFADISYALNICIMYSLPGIVIGKMLKRSFEQEDSNK
                     FEPIYGGTIIFVLSMLVYFFVLKTFLNVNLLDEVSKMISEIVNIQKNSFSATELKVFD
                     KMKPDEIVSYFTNMLPMMLFLQGLLSAFVTYYLSVFFIKRITKMNIRFPKFADFYLPG
                     NAILTTFLLYLLVLFIQIIGSKLYTELIMTNLQLVFNLMFVIQGIAVCIYFLKKWIRQ
                     GPNKIMFFSGIILCLFGFMGISFVGMVDSIIDFRKVRSYKST"
     gene            8349..10343
                     /locus_tag="KRL67_000955"
     CDS             8349..10343
                     /locus_tag="KRL67_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HBH1521860.1"
                     /translation="MSNKQTFKLNMPEINLYIIVIGISSIILLYYNLYVGCLFFCIFV
                     YMVFHNWRTTNIRRHEWTEYIQNLSLDIDETTKKAIINLPIPLCILEFDGNISWYNGK
                     FYDMIGQKDLLDKNIEDIVKNLNLRKVLNENKEMYTEINYKEKEYTIIYNVIKNDQEK
                     NPKYLMILYWIDKTEYLKVKQNYDDEKNAMMLIQVDGYDEVLKSAAEDKRALINVEVE
                     KILSALELNSNGALRRTSKDKFFLVMHKKELKKLEAEKFSILDTIRHIDYGNNLPVTI
                     SIGIGIDGDTLNENLKLATGALDLALGRGGDQAVVKTKDKFVFYGGKSKAVEKKTKVK
                     SRLIGHALREVIQQSDQVYIMGHKYPDMDAMGAAVGVYDICKSCNKTANIVLQSVNES
                     IEIFINKINENNYYKKLFIGKEEAIDNCTKNTLVVVVDTHRPNYTECEELLKLSEKVV
                     VIDHHRRGVEFINDAVLLFHEIYVSSTCEMVTELVQYMDEDVTINKLTAEGLLAGISL
                     DTKNFAFKTGVRTFEAASYLRKVGADTIEVKKFFNSDVKDFIIKAEIIQSTKIINNRI
                     CLAYSSTEIDSINVIIAQTADELLNIKEVEASFVLGEKDDTIFISARSLGQINVHVLM
                     EKLGGGGHIDIAGAQLKNVSLKEAYKMVNKIIEEYLEEEE"
     gene            10344..10793
                     /locus_tag="KRL67_000956"
     CDS             10344..10793
                     /locus_tag="KRL67_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HBH1521861.1"
                     /translation="MKVILLKDVKGTGKKGEMKEVSDGYARNFLFPKKMAVQADSVAI
                     KELNEKNKSKEIKAQKEYEEAVLLGKQMEEINIEIYSKAGEGGRLFGSITSKEIAEQL
                     KKQKDIDVDKRKILLDEPIRSLGSTFVEIKIHQKVTTKIRVDVKEKQ"
     gene            10812..12140
                     /gene="dnaB"
                     /locus_tag="KRL67_000957"
     CDS             10812..12140
                     /gene="dnaB"
                     /locus_tag="KRL67_000957"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HBH1521862.1"
                     /translation="MEDMTRIPPHSVESEQSILGSILLDKDAIITVTETIKPDDFYKE
                     AHKIIYECMITLSNKGEPIDLITLTEELRKQGHLNDIGGISYITSLSTIVPTTSNVKY
                     YADIVKEKSVLRKLIKASNEIINLGYSGATKIEDVLEQAEKSIFDISQEKTSDDFKSI
                     NLVLMDAYDMIEKLYTNKSDVTGITTGFKDLNKKINGLQRTDLILIAARPAMGKTAFS
                     LNLVQNAALKGDASIAVFSLEMSKEQLVQRMLSSQSSVELKKIKTGTLNDNDWPRIID
                     AMAVLSDAKIHIDDTPGIKISELRSKCRKLKIEKGLDLVLIDYLQLMEGEGNNESRQQ
                     EISKISRSLKILAKELNCPVVALSQLSRAPEQRADHRPMLSDLRESGAIEQDADIVMF
                     LYRDEYYHADSESKNIGEVIIAKNRHGETGSVELVWLGEVQRFGDKLRDL"
     gene            12345..12653
                     /locus_tag="KRL67_000958"
     CDS             12345..12653
                     /locus_tag="KRL67_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011862026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH1521863.1"
                     /translation="MEKISLKYIYPNIIKVLDEINLFRVIDNNLRESIVVYANNVDNQ
                     YHINMTNTNFGNIINICKLEKLLDVDKFMEKVIKYEKEIIEKEEFSKIEEYMLNIGEY
                     "
     gene            12735..14024
                     /locus_tag="KRL67_000959"
     CDS             12735..14024
                     /locus_tag="KRL67_000959"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HBH1521864.1"
                     /translation="MKTVAIVGSQWGDEGKGKVIDYLATQADVVVRGQGGNNAGHTLV
                     VEGKKYALHLIPSGVLNPNTVNIIGNGIVFDPKGFLEELEMFKTDNISTENIKISDRA
                     HVIFPYHKELDALSEEARGDLKIGTTKKGIGPCYMDKTERSGIRICDLMDKDKFAIKL
                     KAQIDAKNEIVKNIYGKEELFDFETIYNEYLGYAEQIRKYVADTSVIVYDAVRAGKKV
                     LFEGAQGTLLDLDLGTYPFVTSSHPTSGGFAIGAGIGPNMIKDVVGIVKAYTTRVGEG
                     PFVTEQINETGDKIREQGHEFGVTTGRPRRCGWFDAVIVKYAARVNGLTSISFMLLDV
                     LTGFDKIKVCTSYKMGDKIITDFPASLDDLAKCEPVYEELDGWNEDITQIDNFDDLPE
                     NAKKYIAKIEELVGVSVDMVSVGPNRAQTIIRRNIFA"
     gene            14356..14431
                     /locus_tag="KRL67_000960"
     tRNA            14356..14431
                     /locus_tag="KRL67_000960"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:14389..14391,aa:Lys,seq:ttt)
     gene            14438..14512
                     /locus_tag="KRL67_000961"
     tRNA            14438..14512
                     /locus_tag="KRL67_000961"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:14471..14473,aa:Glu,seq:ttc)
     gene            14517..14592
                     /locus_tag="KRL67_000962"
     tRNA            14517..14592
                     /locus_tag="KRL67_000962"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:14550..14552,aa:Val,seq:tac)
     gene            14626..14700
                     /locus_tag="KRL67_000963"
     tRNA            14626..14700
                     /locus_tag="KRL67_000963"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:14658..14660,aa:Thr,seq:tgt)
     gene            complement(15084..16076)
                     /locus_tag="KRL67_000964"
     CDS             complement(15084..16076)
                     /locus_tag="KRL67_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBH1521865.1"
                     /translation="MNEDKIRKILAKYAKRRDDNELLLEHRKNEVYNRIPEIKSIDDE
                     ISKIGLSLAKIVLLNPKSKDEIVKKTKENIESLNVKKERLLAESNIPLDYLEIKFQCI
                     SCKDKGFLPNGEKCSCLKQEIVNEAYKMSNLDRILSQENFSNFNLNIFSPKKGSDGEI
                     SPRENMLNNLSICENFVHDFKKDNSENLLFYGSTGLGKTYMCNCIAKELLDKGNVVIY
                     QTSFRILDILEDYKFRRDTNNQISEDNYKNLFDCDLLIIDDLGTELNNSFTSGEIFNI
                     VNTRLVAGKKIIISTNLTPSQIGNTYTQRTLSRILDKFRILEFTGDDLRWERFK"
     gene            complement(16085..17143)
                     /locus_tag="KRL67_000965"
     CDS             complement(16085..17143)
                     /locus_tag="KRL67_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HBH1521866.1"
                     /translation="MFFKESNEIDLGEITIPNIFIDIFMPMADGLYVKVYLLGYRQAC
                     DITSNPKFDNNSIAKNLNIPLSDVLSAWKFWEEKKIIKIHDNGEYDNFNYSIEFLDLK
                     NFYIENILSNNSSIKSNTDKVVSTSENPSIRKMFNSINKIVGRYLDPSEKMSIMDIMN
                     KYNMSPDMILCAYEYVKDKTGTSKPVKYIEGIIRNWYDSNLYTPKDVEESFLVRSERY
                     ILYKTIFNELGFSRQPSKSEKELMDTWFDKFNMDIDLIINACSKSKNISNPSISYING
                     IIKNWNEKNIKNLNDLKQKEEERIVKENINKKQINTTQNNNTYKKTKFHNFNETFTQY
                     TSDELDEIIKKSQKEKFK"
     gene            17268..18731
                     /locus_tag="KRL67_000966"
     CDS             17268..18731
                     /locus_tag="KRL67_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HBH1521867.1"
                     /translation="MVIKFNQKVRRTLSVAILFMVLIIGCFIEKDDSLETYKEKTNSK
                     INQYNMDVIFDDETKRLMCNQNVDYINNTKSNIDKIYFHIYPNAFSKKDFAPFEKSEM
                     TRAYPNGFNEGYIDIKNILNNNSKMEYKIKGDKNDILEIELGKELKPNERMSLDIKYN
                     VKIPNSVGRFGYGENTINVTNWFPIACVNDERGWNLKSYETIGDPFYSETSDFHVKLL
                     IPNKYKIAHTGKLIDDKHDNKKMLYEIEAENVRDFAFILSSKFDVNKVDSKGIAINTY
                     NLNKKLSKKATEVAKDSINIFSELFGKYPYKEFSVVASDFFIGGMEYPMLVMIDQSLY
                     NEKNEFLLEYVIAHETAHQWWYSAVGNDEISEPWLDEALTEYSTIVYFEQKYGKDMAN
                     KLIKTMEIQTKSYLSENIFKPANEYKNSTEYSLNVYTKGAIAFNEIRKEVGDKVFFET
                     LNEYYNKYKHKNANGRAFVELWNSKGVDINKIISECK"
     gene            18816..19613
                     /locus_tag="KRL67_000967"
     CDS             18816..19613
                     /locus_tag="KRL67_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HBH1521868.1"
                     /translation="MLKYCSVGSGSSGNCHYIGYKNTNILVDAGLSGKRITTGLKDID
                     VDADKLKGIFITHEHVDHIKGAGILSRKFDIPVFANIKTWCSMKDKLGDIKDKNMKVF
                     ENDKTYSLGDIIVRPFSIEHDASDPVGYNFYTESDEKMSIATDIGCITQNIKKHLYKS
                     KLVVLESNYDPNMLMMGSYTYALKKRVMSSTGHLSNEDAANFCVELINEGTESILLAH
                     LSKENNFPELAYETSKGVLASNDIVVGQDVKLDVLSRNDVSNVYEMK"
     gene            19626..21728
                     /locus_tag="KRL67_000968"
     CDS             19626..21728
                     /locus_tag="KRL67_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC/EAL
                     domain-containing protein"
                     /protein_id="HBH1521869.1"
                     /translation="MLKRNFENVEDNKILYSIKQGMILAIPAIMTGSTALVILNLPIK
                     AYQEYLSSLFNGEVTNILNFINDSTLGIISLIILLTVSYSYGKIHGSKYTVLVPIVAM
                     CSFLVFSHSNELNSYIEIFKTKWLFTAIIVSMTSSVLFVKLTESFVTSVKFHTEGADA
                     DFNMVVSAIVPFIIVVFLFSIFRVIMISLIGSSNFQDIFYNLFSNVFNRMGTNLMSAL
                     LFIFLMHFMWFFGIHGSNVLDTVAKNLFENSMAININLVNNNQLPTEIFTKTFFDTMV
                     LLGGCGSLLCLVIAIFLSEKRTNVRKLAKIASIPALFNINEMLVFGIPIVFNYIMFVP
                     FVITPIILTITSYVAMSTGIVPCTVSAVEWTSPIFLSGYMSTGSIRGSLLQLFNLCVG
                     VMIYIPFIKMSQNRYTYMFKNNIENLTNLVKRYELTGEQPNLLNHNGKIGSISKMLAS
                     DLKFAMKRGEIELFYQPQVNYNGDVVGAEGLLRWKHSIGGFIYPPLVIILAKEEGFLD
                     ELGEYIINKACIDLKKSEKLLRNPVKFSVNISPDQLDDPKLPEQIKNIISSNDINPNM
                     LGIEITEQIALSGSQIIIERINAIHNLGIKLIMDDFGMGHSSLIYLQNNNFDIVKLDG
                     SLVKEILTNKRSSEIIMTIVNLSKNLDFDIVVEYVENIDQRDKLYGLGCEIYQGYYYS
                     KAIPFEEFVEYANQEREI"
     gene            21796..22275
                     /gene="rlmH"
                     /locus_tag="KRL67_000969"
     CDS             21796..22275
                     /gene="rlmH"
                     /locus_tag="KRL67_000969"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001027003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HBH1521870.1"
                     /translation="MNISIVVVGKIKEKYLKLGIDEFKKRLSKYCKLEIIELDDEKAP
                     ENLSIKEMEIIKYKEGKKILGKIKHNSYVIALAIDGKGLSSEELAKTMSDLAVRGNSS
                     LCFIIGGSLGLSDEVLGRADYRLSFSRMTFPHQMMRLILLEQVYRAYRINNGEPYHK"
     gene            22424..22735
                     /locus_tag="KRL67_000970"
     CDS             22424..22735
                     /locus_tag="KRL67_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBH1521871.1"
                     /translation="MIKIMLACSAGMSTSLLVTKMESAAKENGIESQIWAIPESTIQN
                     EIEKCDVLLLGPQVRYVLPKAQEIAKPYNIPVEVINMMHYGTVNGEAVLNRAIELNNT
                     K"
     gene            22783..23100
                     /locus_tag="KRL67_000971"
     CDS             22783..23100
                     /locus_tag="KRL67_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HBH1521872.1"
                     /translation="MDEKIIEISFNIIGYAGEGKGLAFEAIKEAKNGNIEKAKELLKE
                     SKEVINKAHRYQTELIQNEASGNKTEISVILIHAQDHLMNGMNFQQLAEEIVDLHLKL
                     QDK"
     gene            23313..24014
                     /locus_tag="KRL67_000972"
     CDS             23313..24014
                     /locus_tag="KRL67_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBH1521873.1"
                     /translation="MLRNQPLYIQVVDILKQKILNNEYKIDENIPTERELEQEFGVSK
                     ITIRKAIEILESEGYVEKKSGKGTKVISNSLFNKLSKAESFSSILLSKGHKLSKETIS
                     IEKIINDTNPQVFSIFGEKCSKITRMYYLDSKPYIFFTHYIPFDENLTVESFNNESSL
                     YMYLYRRNINITRFEDEFFIEESELEVSKALNLAKDILLGRKRKTYDENDNIVELSFA
                     RYNTEVQNYVIKYEI"
     gene            24238..25539
                     /locus_tag="KRL67_000973"
     CDS             24238..25539
                     /locus_tag="KRL67_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBH1521874.1"
                     /translation="MEKFASFLERKIMPVAAKISNQRHMRAVRAGIIATLPLTIVGSF
                     FTILLNIPIDAYMDFIAPFKATLDIPFRYTVGFLSLYATFGIASSLAKSYDLDTTGVG
                     MLAVMAFLVSTIVPTQVVEPVGGVIEAGRWMDMAKLNSPSLFGAIVTSLITVEIYRFM
                     KEKNITIKMPAGVPVEVSNSFTALLPTAVILMLFWVIRHILGFDISAALSAMLSPLKG
                     FLSGNSLGGGLLTVFLITLFWVLGIHGPAIMGPVIRPIWDMSIAENITAFTEGVSAHA
                     MPNIFTEQFLQWYVWIGGAGATLALVVMFMFSKSQYLKSLGRLGFLPGLFNINEPIIF
                     GAPIVMNPILGIPFIIGPIIMTILSYVLTITGVIPMMVARLPFAMPAPIAAVMSTNWS
                     ILAGILVLINFVISFAVYYPFFKVFEKQQLQREQEEMSENL"
     gene            25676..26770
                     /locus_tag="KRL67_000974"
     CDS             25676..26770
                     /locus_tag="KRL67_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HBH1521875.1"
                     /translation="MERRLGISVYPEHSNMEDDKAYIKMASKYGFKRIFMCMLSITRP
                     KEEVKKHFKEIITYARELGFEVILDIAPNIFDTLQISYDDLTFFSELNASGIRLDYGY
                     DGSKEAMISFNPYDLKVELNMSNDVAYLDNIITYKPNVNNIYGCHNFYPQRGTGLPYD
                     FFVRCSNRFKKYGIRTAAFVNSKYATIGPWNINDGLCTLEMHRNLDIATSTKHLFATG
                     LIDDVIVGNSYASEDELKRMSEVNKYQVVFNVVAEEKTSEVEKIIMVNEQHFRRGDIT
                     NQVARSTEVRKKYSKENFPKHDNKNIFKRGDVVIGNDDFGKYKGELQLILQDCTDERK
                     NKVGRIKEDEIILLDFIEPWSKFKIILEGE"
     gene            26772..27905
                     /locus_tag="KRL67_000975"
     CDS             26772..27905
                     /locus_tag="KRL67_000975"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase/deacetylase family
                     metallohydrolase"
                     /protein_id="HBH1521876.1"
                     /translation="MKIDILIKCGKTVDKSIIDIAILDDKIIEVKNQIDASKYQAKQI
                     INLDGEKFISAGWIDIHTHCYERLDLYKDYPDEVGIKSGVTTVVDAGTTGALDIGKFY
                     EDTKKYKTNVYALINIAKQGITSQDELSNMMNIDENELKNAVRKYKDFVVGIKARMSK
                     SVVISNDVEPLKVAKRIKNELNLPMMVHFGSSPPTIEDIFDYMEKGDILTHIYNGKPN
                     GILRGNDVKKEIIEARERGIILDVGHGTESFSMDVAMKSKDAGIFPDTISTDIYIKNR
                     INGPVYNLSTTMEKFIYMGYSLEDIIDKVTKNAADAISLKNKGLIKEGYDADLTIFDM
                     VNEEKELQDSLNKSVITSTSIKPRAVVVNGEYLNIDKSVEENE"
     gene            27923..29032
                     /locus_tag="KRL67_000976"
     CDS             27923..29032
                     /locus_tag="KRL67_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DgaE family pyridoxal phosphate-dependent
                     ammonia lyase"
                     /protein_id="HBH1521877.1"
                     /translation="MSSIFEKYSVRRVINASGKMTILGVSTVDKEIIEAIGEGCKNFF
                     IIEELVNKTGEYIANLLGCESALIVSSASAGIAQSVGAMIAKNDMSLVYNVNNPKKQV
                     KREIIIPKGHNVDYGVPVEVMIRLGGGEVVEAGYANMCNKEHIIREINENTAAIMYIK
                     SHHCVQKSMLSVLDAVQVAKEYNLPLIVDAAAEEDLNVYYELGADLIIYSGAKAIEGP
                     SSGLVIGKKKYIDNVKLQSKGIGRAMKIGKENIVGLTCAIERYISNDKVTLKEMEDKL
                     NPFIDKINTLKGVSASITRDSAGREILRGEIDFNEDIINKSTQQIINELRSGEIAIYT
                     RDYKANEGKVEIDIRSVIGSDLDIIYNRIKNIVES"
     gene            29082..29837
                     /locus_tag="KRL67_000977"
     CDS             29082..29837
                     /locus_tag="KRL67_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001186146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KDGP aldolase family protein"
                     /protein_id="HBH1521878.1"
                     /translation="MEEINMNCIPQYYKNRVCLNVLAGSIRNAKEVYNACEGHVLVGV
                     LSKNYSTVESAIEDMKKYSKEIDNAISVGLGAGDPNQSNMVSQISKELQPQHINQVFT
                     GVATSRALLGQNDSIINGLVSPTGKPGFVKISTGPLSSKEKDGIVPIETAIAMMKDMG
                     GSSIKFFPMGGLKTKDEYVKVAKACAKHNFYLEPTGGIDLDNFKEIVQIALDAGVEKV
                     IPHVYTSIIDKETGDTKVEDIEVLYKIMKELLG"
     gene            29842..30834
                     /locus_tag="KRL67_000978"
     CDS             29842..30834
                     /locus_tag="KRL67_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HBH1521879.1"
                     /translation="MNILAFGEIMMRLSVPDYKFLTQTNELNYVITGTGLNILSGLKN
                     FGYNTYMLTKLPNNNVGKASSANIRKLGVKDDFITYGGNHIGVYFLESGYGERPSEVT
                     YLNRLNSSFCESKLQDYDINKCLEGMDVVHICGIAMQLTDSVKEIALELARKSYEKGI
                     KVFFDFNFRQSLNPEKTYDDLVNDYKKMLPYCHGLFASYRDINKILNIDNLDIKSSSE
                     LIKKEYDIDIVAGTMRRFDDMDNRFIKGYISNDDGYFESKEYKLEIYDRVGAGDGYVA
                     GIIYSYLENMSNEDIVEFGVASSVLAHTTYGDNSLVSTSQVLRLMNNEKIDILR"
     gene            complement(31021..31902)
                     /locus_tag="KRL67_000979"
     CDS             complement(31021..31902)
                     /locus_tag="KRL67_000979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBH1521880.1"
                     /translation="MNTKQLEYFISVAENLSFTKTAEKFYISQTAVTQQIKALEEQIN
                     VTLFTRSKRHVELTPAGKVFLSEARTIIKNINDAIAKTQQFAHGFFGTLSIGTLIGYE
                     KNKLQQYLKEFSNTYPNISMDICTNEITELLNLVKNNSMDLAFVINPENQPLKDFEYK
                     TVERYSLVALLPPGHPLYNEDSIDLIELQHDKFIFVKETGDEYGQKSMIQNRYREAGF
                     VPNVVQRSNNLNTIESLVASNMGISILPSFCITDTMIERDIAIVPIKEVENRIEVVVV
                     WNKKNTNPALEKFISII"
BASE COUNT        12536 a         3464 c         5574 g        10382 t
ORIGIN      
        1 aataaatgtg tttatatagt aactgcatat attactagtt taaaaggaca ctattcattc
       61 gtagaaactc aaattgatga ttttattaaa aaatatgtag aagatattgt agatttatgt
      121 cttcatagaa taatgattct attatatgaa tgtgacttaa cataaaatta gatatattaa
      181 agttgagtga gaaaaatctt gaataaagtt cttcttacca actttaatat atttctatta
      241 tacaatctat tttttatcat tattgaaatt gtttacaact tgatttaatt caattaatat
      301 acttccaact tccatataga gaatcttact ataaacacaa gtaatacaat aggaacgtaa
      361 ttatcacttg atttttttat tttttaaatc ccataacaaa acattaagtg caactattgg
      421 agttaacaat tgttgcagta taaaagctat taagttatct atatattatc ttcctgaaaa
      481 tattttctta acactttaat tattattcaa aaactattta aacaaagcta taagagagtt
      541 taagttatta ctccaataag atttattctt tattttatac atttattttt tcaaaaacta
      601 taaaagaaca gtatattttt tagacgtaga atttataact aaagattata atattgctat
      661 tgaagctaag cattataaaa aacattggat acaatttatt gatatagcat tttcaaaatg
      721 tcaatatcac ttgttatatt actgtaagtt gtatagatta tgttagcctt gaaagtattt
      781 gtgtatatat ggtgactatt ataactgaca atctggtaaa taagataaag ataacgttag
      841 tagggtaaaa tattgctcta taaaagatat aaatagaata tgaaaggtgt tatttcaaaa
      901 ttgatttttt ataatcaaat ttttatttct atttttctgt ttttatatgt aaaaaagaaa
      961 ggaaggagat gattcaaatt tttattttga atcatctcct tagatatgat aatttataac
     1021 agtttcaaga aattacttta agtaatctcc ttcttcaaga actatatggc cactttcgta
     1081 attagaacca tcaacagtaa tttttatttg ttcaacattc atatttttta tgtaagtacg
     1141 agcaactgaa tcaagcatta aagtttctgc atcactacct aagtttgact ttgtaaagtc
     1201 agagctaaca tctaatatcc cagtttttac gccatcaatg tcttttgtag taaatgaatt
     1261 taattttgca gattctggaa caactttaag tttttgaagt tcttcaaata aagtcttctc
     1321 atcaatttta cttaaatcaa ctttattttc aattaaatct gtcttatcta catcaaaaga
     1381 gtaaatagta gctgattgag ttttagtttc ttcttttgga gtaggtttag ctttgttatt
     1441 aggaacctct ttttcagtat tgttttcttc ttttggttgt tcaacatttg tattgctctt
     1501 agtttctttt ttagatgtat catttccatt ttgagcatta gtacaaccaa ctgcgaatat
     1561 agatatagat aatatactta acactaacat tattttctta tttttcataa aaatttctcc
     1621 cctcttaaaa actattctta tatataatta tagtcgttat gagcaaatat atgaatacaa
     1681 aatagtaaca aatttaaaaa atgatttatt taaattaata aaactcaaaa taaactaaat
     1741 tttagtcatt tttatgggaa atcatttaat gtacactgaa aatattgtaa aataaaagta
     1801 gtgtttttaa aataaatata agttataatt atattataaa aaaatgtctt caaaaggtgg
     1861 gaatagtatg gaaattttga gcttaggaga aaagattaaa aaattaagaa aagaaaaaaa
     1921 catgacatta aaagaattag ctggagatag gataacagca gcacaaataa gccatataga
     1981 gagggataaa tctcatacta gttatgaact gttggaatat ctaggcgata agctagatgt
     2041 aagtgtagaa tacttattag agaccaagga aatgcaatca aaaaagataa ctgataattt
     2101 aatactacag agcgaaattt atataaaaga aaatgaatta aacaaggctg aggaccaaat
     2161 aaaagaagtt ttagatatat gtaagagata cgatttaatg gagaactatg gcagatgtaa
     2221 ttttttaatg gctaatatta atttgaaaaa atctaattat aatgatgcta tagaaaactt
     2281 tgaaaaggca ttgtacttct ttataaaaaa tagtgatagt gaaaatatac ttaagtgtta
     2341 tttaaacata gggaaaatat atatgcagga agacttttat aaaggtgcta taagccactt
     2401 taattttgca gaggaaattt tgtcagagaa taaatttgag gatgttaaca tacacaagga
     2461 actatatagt aagatggcat attgttacat aaaactagat aatccaaata tgtcattgaa
     2521 gtacatagaa aagataaatg aaatagataa tagaaatgat agaaaagaag atgtcgatat
     2581 tttggtgtta aaagcaaata atatgcttga gattggaaaa tatgaagaat caagagatta
     2641 ctttaaaaaa gcactaaaaa tacttgagag tgaggataat aaaactggat tagcaaatgt
     2701 gtatttaaca atttgtgatg tttacagaaa aataggtgaa acagatagag tacttgaata
     2761 ttcacaaaag gtatatgaca taaagaaaaa tgatgaagat gaatacatga tgtcaggatt
     2821 atttaagata atagaagcat atatagataa agaagattat gatttagcta gaaaatattg
     2881 taagatagcc ttagcttcat caataaaaaa taaaaataaa ttcaatgaat ataaagcatt
     2941 gagattctat gctaatatgt ataaaaatca gaatgaagta actcttgcaa ttgaatatct
     3001 gattaaatgc ataaaaatag tatcagattt aggtaacaat agaatattgg ccaatttata
     3061 tatagactta ggtcaattat attcaaatat atcaaaagaa aaagagttag aatattatca
     3121 aaaaggtgta tttatgtaca aaaatttaga aataatgtag ggtaaataaa cctataaggg
     3181 tatagaaaca atctataaca agcaagataa tatctatgat aaaatataaa ttgtatgaat
     3241 atttttaagg agatgaatca gacaatgaat ataaaaatag atgcaagagg gcttgcttgt
     3301 cctaaaccag ttataaacac aaaaagagaa ttggataacc tagaagaagg tgttgtagtt
     3361 acaatagttg acaatgaaac agctaaggag aatatattaa aacttgctaa atctttaagt
     3421 tgtgaagcaa atattgttga cgaaaaagag aattttatat ctatagaaat tagaaaaggt
     3481 aaaaatatac tagataaaca aaatgttgta gataataaag aaagtgaatt agacaataca
     3541 tgtatattta tatcttcaga taagatggga cttggaaatg acgaattagg aaaagtatta
     3601 ataaaaggat ttatatatac attaacagaa tctaaacctt atcctaagta tgtactttta
     3661 gttaatggag gagtaaaatt aagtgctgaa aatgaagaaa ctatagaaaa tctaaaaata
     3721 cttgagaaga tgggtgtaga agttttatct tgtggtactt gtttagatta ttataattta
     3781 aaagacaaac tgcaagtggg tacggttaca aatatgtata ctatagttga gacattaaag
     3841 aatgcatcaa ataccatttc tatataatgt atgtttgttt aataatataa aactaataat
     3901 aaagattaac tcaaatagat agtcagaaag gattaagtaa tgaatcaaat gtatatagtg
     3961 tcatttaatt cgacacatca tgcgataaga tcagaaaagt tatttggtga aaatagtcta
     4021 aaagtaatgg ctcttccaac accaagagaa ataacagcaa gttgtggaat atcaattaaa
     4081 ttttcttttg aagatatgga aaagataaaa actattttgg ttaaaaataa tgtagatata
     4141 aaaggaattt attgtataag taaattagaa aatggttcaa aagaagtaga gaagttagat
     4201 taggaggcta attatttatg ccaatggatt taaaaatagg tgatattgtt gaattaaaaa
     4261 agcaacatgc atgtgggtgt aaagagtttg aaatcataag aacgggaatg gatgtaaaga
     4321 taaaatgtac taaatgttca aggctaataa tgcttgatag agagacctta gaaaagagag
     4381 taaaaaagct aataaaaaag tgataaaaat ttaacactta aaaaatgtca aataccgtat
     4441 attaatatcc aaaattttgg tataataaga attgaagaaa acattttcat gggggtgctt
     4501 taattatggc agttaaatat gcaaaaagaa tgcaaggatt acaaggatca gaaatacgtg
     4561 agcttttaaa acttactcaa caaccacaaa taatatcttt tgctggtgga atgccggctc
     4621 cagagttatt tccagttgag gaaatgaaaa aagtatcagt tgcagtgctt gaagaaaatg
     4681 gaagatcagc tatgcaatat actacaacag aaggatatga accattaaga gaaaaaatag
     4741 cagctagaat gaatgataaa aacaaaacta atgtaaacaa agatgatata ttagttacaa
     4801 gtggttctca acaaggtcta gattttgcag gaaaagtatt tattgatgag ggtgatgtaa
     4861 ttttatgtga aagtccatct tacataggag ctataaacgc atttaaatct tatcaaccta
     4921 aattcataga tgttccaaca gattctgatg gaatgataat ggaagaatta gaaaaaatac
     4981 tagaaacaac tgatagaata aaaatgattt acgtaatacc tgacttccaa aatccaacag
     5041 gaagaacttg gccacttgaa agacgtaaaa aattcatgga aatagtaaac aaatttgaaa
     5101 taccagtaat agaagataat ccatatggag atttaagatt tgaaggagag actttaccat
     5161 ctttaaaatc aatggataca aaaggattag taatattctt aggaactttc tctaaaatat
     5221 tctgcccagg atacagatta ggatggactt gtgcttctcc agaaatttta tctaaattta
     5281 actttgcaaa acaaggtgca gacttacaag catcaactat atctcaaatg gaagtaagca
     5341 aatttatgga tatgtatgac ttagatgcac atgttgacaa aataaaagca gtttatgtta
     5401 aacgtagaga tgtaatgctt aagactatgg aagaagaatt cccagaagga ttagtattta
     5461 ctcatccaga aggtggatta tttacatggg tagagcttcc aagtaattta aatgctaaag
     5521 aattaatgcc aaaatgctta gataagaatg ttgcttatgt tgctggagga ggattcttcc
     5581 ctaatggtgg tagagaaaat actttcagac ttaactattc aaatatgcca gaagaaaaaa
     5641 taatagaagg tataaaaaat atagcagctg tattaaaaga agctatgggt gtagaagctt
     5701 aattaaaaat aataacttat atataataca agggatggct gttgccatcc cttgtttgtt
     5761 tgaaaaatat ataattcacc ttatactaaa ctttgtccaa aatttaaatt gacagaaata
     5821 agaataaatg ataaaatgtg taggtatgag attttagtct catttctctg ctctaagagt
     5881 gtagagccgt taagtccaaa gggaggtgaa ttatagtgag aaattatgaa ttagtttatg
     5941 tggtaaagcc aaactctgat gaagaagtaa gagaggctat actaaacaaa gttaaggaag
     6001 ttgttgcaac tgacggagaa atagttaaag ttgatacttg gggaactaaa aaattagctt
     6061 atccaatagc taagtttaca gaaggtttct atgtattagt taacttcaaa tcagcagtag
     6121 acgttcctaa agagatagac agaaacttaa agataaatga aaacgtaata agacacatga
     6181 tagttgttgc ttaataactg gttttattat aaaaaatagg tggtgttttt atgaatcaag
     6241 ttgtattagt gggaaggttg actagagacc ctgaattaag atacatccca ggtacaggaa
     6301 caccagtggc atcctttaca atagccattg atagagacta tgtaaaaaaa gatggatcta
     6361 aagaaactga ttttatacct gttgaagtaa tgggtaaatc tgctgaattt tgtgctaact
     6421 acataactaa aggtagatta gtagcacttc aaggatctat aagagtagac aattatcaga
     6481 cacaatcagg tgaaaagaga acttttacaa aagtaagtac tagatcagta caagctttgg
     6541 atagtaagaa taaatcggaa aattcatata gagaaagtgc tccagctttt gagccaagct
     6601 ttgaacctca aggtttagac ccacaaggtt tccaagctat agatgatgac gatataccat
     6661 tttaaaaagg agggaaaaaa gtcatgatga ataagaaaag acgtaagaaa aaaagagttt
     6721 gtcaattttg tgctgacaaa aacgctaaaa tagattacaa aagtactcaa agattacaaa
     6781 agtatataac tgaaagagga aagatattac caagaagaat atctggaact tgtgctaagc
     6841 atcaaagaga attaacagtt gctataaaaa gagcgagaaa tatagcactt ttaccatata
     6901 cattagacta aaaaaatgag ccttgtgctc attttttatt atatgtattt atatatctaa
     6961 atgaggcgag ctataaagct cgtttttttg tttatatact taaatatatt attatttatg
     7021 atataatatt atatattaag gagggttgta aacgtgaaaa tagataaagg ttctgaaata
     7081 acgagaaata taaagataat agaatggatg aaaacagaaa tattaatgag tgtaagtgat
     7141 ttgtttaatt tattgtttaa aggggtaaaa ccattagatg aagcaattca agatacttta
     7201 gcaaatataa taatgataac atatctctta gcaaagagat taggaattag ttttagagat
     7261 gtggattata aagtcaagga aaaaataaaa atcggtatag atgaggacca tagcgttgaa
     7321 agatggtatg gagatttatc taatttaaaa aaacatatgg ataataggga gtgatattaa
     7381 attgaataat aaaattagac tatctaaggc aatgtctata atagttttag ctataataat
     7441 cgctttagta atagcctata ttccaatgct aggaatattt agtatgttag cagctgttcc
     7501 atatgtaatt gtaggtgcta ttactgatag aaagtattct cttatatcta ttttggttac
     7561 attttgtgta ctgattctat ttgcagacat ttcttatgca ttaaatatat gtataatgta
     7621 ttctttacca ggtatagtaa taggaaagat gctaaagaga agctttgaac aagaggacag
     7681 taataaattc gaaccgatat acggtggaac cataatattt gtactttcaa tgcttgtata
     7741 tttttttgta ttaaagacat ttttaaatgt gaatctatta gatgaagttt ctaagatgat
     7801 atcagaaatt gtaaatattc aaaaaaatag tttttctgca actgaactta aagtgtttga
     7861 taaaatgaag ccagatgaga ttgtaagtta ttttacaaat atgctaccaa tgatgttatt
     7921 tttacaaggg ttgttatcag cctttgtcac atattatttg agtgtatttt ttataaaaag
     7981 gattacaaag atgaatataa gatttcctaa atttgctgat ttttatttac cgggaaatgc
     8041 tatactcaca actttcttac tttatttatt agtcttattt attcaaatta ttggttcaaa
     8101 actatataca gaattaataa tgactaattt acagctagtt tttaatttaa tgtttgtaat
     8161 tcaaggtata gcagtttgca tttatttctt aaagaaatgg ataagacagg gaccaaataa
     8221 aattatgttt ttttcaggga taattttatg tttatttgga tttatgggaa tatcttttgt
     8281 tggtatggta gatagtataa ttgactttag aaaggtaaga agctataaat ccacttagga
     8341 ggatagaaat gagtaataaa caaaccttta aattaaatat gccagaaata aatttatata
     8401 taattgttat tggtatttct agtataattt tgctttatta caacttgtat gtagggtgtt
     8461 tatttttctg tatttttgta tatatggttt ttcacaattg gagaactact aatatcagac
     8521 gtcatgaatg gacagagtat attcaaaatt tatctttaga tatagatgaa acaactaaaa
     8581 aagccataat aaatcttcca ataccattat gtattttaga atttgatgga aatatatcct
     8641 ggtataatgg aaagttttat gatatgattg gtcaaaaaga tttacttgat aaaaatatag
     8701 aggatatagt taaaaatctt aatttaagaa aagtattaaa tgaaaataaa gagatgtaca
     8761 cagaaataaa ctataaagaa aaagaatata caataattta taatgttata aaaaatgatc
     8821 aagaaaaaaa tcctaaatat ttgatgatat tatactggat agacaagact gaatatttaa
     8881 aagtaaaaca aaattatgat gatgaaaaaa atgcaatgat gctaatacaa gtagatggat
     8941 acgatgaagt attaaaaagt gcagcagaag ataagagagc tttaattaat gttgaagtag
     9001 agaaaatact ttctgcttta gaattaaatt caaatggtgc attgagaaga acttcaaaag
     9061 acaaattctt cttggtaatg cataaaaaag agttaaaaaa attggaagct gagaagtttt
     9121 ctattttaga tacaataaga catattgatt atggaaacaa tcttcctgtt actataagta
     9181 taggaattgg tatagatgga gatacattaa atgagaacct aaaacttgct acaggtgctc
     9241 tcgatttagc acttggtaga ggtggtgacc aggctgttgt aaaaacaaaa gataaatttg
     9301 tgttttatgg aggaaaatct aaagctgtag aaaagaaaac caaagtaaaa tccagattaa
     9361 taggtcatgc tttaagagaa gttatacaac aaagtgacca agtttatata atgggacata
     9421 aatatcctga tatggatgct atgggagcag cggttggtgt ttatgatata tgtaaatcct
     9481 gtaacaaaac tgcaaacata gttttacaat ctgtaaatga atctatagaa atatttataa
     9541 ataaaataaa tgaaaataat tactataaaa agttgtttat tggaaaagag gaagctattg
     9601 acaactgcac taaaaataca ttagttgtag tagttgatac tcataggcca aattatactg
     9661 aatgtgaaga actgttaaag ctatcagaga aggtagtagt tatagaccat catagaagag
     9721 gtgtggagtt tataaatgat gcagtgcttt tattccatga gatatatgta tcctcaacgt
     9781 gtgaaatggt tacagagtta gttcagtata tggatgaaga tgtaacaata aataaactta
     9841 cagctgaagg acttttagca ggtataagtc tagatactaa aaactttgca ttcaaaacag
     9901 gtgttaggac ttttgaagct gcttcttacc ttagaaaagt tggagcagat actatagagg
     9961 tcaagaaatt cttcaattct gatgtaaaag atttcataat aaaagctgaa ataattcaaa
    10021 gtactaaaat aattaataat agaatatgtt tagcatattc aagtactgag atagatagta
    10081 taaatgttat aatagctcaa actgctgatg agttattaaa tatcaaagaa gtagaagctt
    10141 cctttgtatt aggtgaaaaa gatgatacta tatttataag tgcgaggtct ttaggacaaa
    10201 taaatgtaca tgtacttatg gaaaaattag gaggcggggg acatatagat atagctggag
    10261 cacagttaaa aaatgtttct ctaaaagaag cttataagat ggtaaataaa ataatagagg
    10321 agtatttgga ggaggaagaa tagatgaaag ttatattatt aaaagacgta aaaggaactg
    10381 gtaaaaaggg agaaatgaaa gaagtaagtg atggatatgc aagaaatttc ttatttccta
    10441 aaaagatggc tgtgcaagca gatagtgtag caataaaaga attaaatgaa aagaataaat
    10501 ctaaagaaat aaaagcacag aaagagtatg aagaagcagt tttattaggt aagcaaatgg
    10561 aagaaattaa tatagaaata tattcaaaag caggggaagg tggaagatta tttggttcaa
    10621 taacttctaa agaaattgct gagcaattaa agaaacaaaa agacatagac gtagataaaa
    10681 gaaaaatatt attagatgaa ccaataagat ctttaggttc aacttttgta gaaataaaaa
    10741 tacatcaaaa agtaactact aaaataagag tggatgtaaa agaaaaacag taataactag
    10801 aggtgattaa gatggaagat atgacgagaa ttcctcctca tagtgtagaa tcagaacaat
    10861 caatattggg ttctatactt ctagataaag atgctataat aacagttaca gaaactataa
    10921 agcctgatga tttctataaa gaggctcaca aaataatata tgaatgtatg ataactttaa
    10981 gtaataaagg tgagcctata gacttgataa cattaacaga agaactaaga aaacaagggc
    11041 atcttaatga tattggagga atcagttata ttacaagtct ttctactata gtaccgacga
    11101 cttcaaatgt aaaatactat gctgatatag taaaagaaaa gtctgtactt agaaagctta
    11161 taaaagcttc taatgaaata ataaacttag gatatagtgg agcaactaag attgaagatg
    11221 ttttagaaca agctgaaaag agtatatttg atatatcaca ggagaaaact agtgatgatt
    11281 ttaaatctat aaacttagtt ttgatggatg cctatgatat gatagaaaaa ttatatacta
    11341 acaagagtga tgtgactggt ataactactg gttttaaaga tttaaacaaa aaaataaatg
    11401 gtcttcaaag aactgattta atattaattg cagctagacc agcaatgggt aaaactgcct
    11461 tttctttaaa cttggttcaa aatgcagcct taaaaggtga tgcctctata gctgtattta
    11521 gtttagagat gtctaaggaa caattagtac aacgtatgtt atcttcacaa tctagtgtag
    11581 aattaaaaaa aataaagact ggaacactta atgataatga ttggcctaga atcatagatg
    11641 ctatggcagt attatcagat gccaaaatac acatagatga tactcctggt ataaaaatat
    11701 cagagctaag gtcaaagtgt agaaagttaa aaatagaaaa aggactagat ttagtgctta
    11761 ttgattatct tcaacttatg gaaggtgaag gtaataatga aagtagacaa caagagatat
    11821 ctaagatatc aaggtcacta aaaatattag caaaagaatt aaactgtcct gtagtagcac
    11881 tatctcagtt atcacgtgca ccagaacaaa gagcagacca tagacctatg ttatctgact
    11941 taagagagtc tggagcaata gaacaggatg cagatatagt tatgttctta tacagggacg
    12001 aatattatca tgctgattct gagagtaaaa atataggaga agtaataata gcaaaaaata
    12061 gacacggtga aactggttct gtggaacttg tttggcttgg tgaagttcaa agatttgggg
    12121 ataagttgag agacctataa tctaacgaat atccacagaa cgtcctttct ttctacagga
    12181 aaaatgtgga tacatgtcac taaatatgtc caaacattga aatttagcta aaaaattatc
    12241 cacactatcc acagatggcc tgttgataag tgtgaataag tagataaagc aagcttttgg
    12301 cttgctttat ctgtgtataa atattaatta ttaggaggaa taatatggaa aaaataagct
    12361 taaaatatat atatcccaat ataattaaag tacttgatga aataaactta tttagagtaa
    12421 ttgacaataa tttaagagaa tctattgttg tttatgctaa taatgtggat aatcagtatc
    12481 atataaacat gactaataca aactttggaa atataattaa catatgtaaa ttagaaaaac
    12541 ttttagatgt ggataaattt atggaaaaag ttataaaata tgaaaaagaa ataatagaga
    12601 aagaagagtt ttcaaaaata gaagaatata tgttaaatat tggagaatac taaaaaagtt
    12661 gaaaaaaata cgaatgtttg atagaataag taagtaaagt gttcgagagt atataaaata
    12721 tagaggtgaa ttacatgaaa acagttgcaa ttgttggttc tcaatgggga gatgaaggta
    12781 aaggtaaagt tatagattat cttgctactc aagcagatgt agtagtaaga ggacaaggtg
    12841 gaaataatgc aggacataca ttagttgtag aaggtaaaaa atacgcatta catctgattc
    12901 catctggagt tttaaatcca aatacagtga atataatcgg aaatggaata gtgtttgacc
    12961 ctaaaggatt tttagaagaa ttggaaatgt ttaaaacgga taatataagt acagaaaaca
    13021 taaaaataag tgatagagca catgtcatat tcccatatca taaagaacta gatgcattgt
    13081 cagaagaagc cagaggcgat ttaaagatag gtacaacaaa aaagggtatt ggaccttgtt
    13141 atatggataa aacagagaga tctggtatta gaatatgtga tttaatggat aaagataaat
    13201 ttgctataaa attaaaagct caaatagatg ctaaaaatga aatagttaaa aatatatatg
    13261 gaaaagaaga attatttgat tttgaaacta tatataacga atatctagga tatgcagaac
    13321 aaataagaaa atatgtggca gatacgtctg ttattgtata tgatgcagta agagcaggta
    13381 aaaaagtatt gtttgaagga gcacaaggga ctttattaga tttagattta ggaacttatc
    13441 catttgttac atcttctcat ccaacatctg gtggatttgc aattggtgca ggtataggac
    13501 caaatatgat aaaagacgtt gttggtatag taaaagcata tactacaaga gttggagaag
    13561 gaccgtttgt tacagagcaa ataaatgaaa ctggagataa gataagagaa caaggacatg
    13621 aatttggggt aactactggt agaccaagaa gatgtgggtg gtttgatgca gttatagtta
    13681 agtatgcagc tagagttaat ggcttgacta gtatttcatt tatgttactt gatgtattaa
    13741 ctggatttga taagataaaa gtttgtacgt cttataaaat gggcgataaa ataataactg
    13801 atttcccagc ttcattagat gatttagcaa aatgtgaacc tgtatatgaa gaattagatg
    13861 gatggaatga agatataact caaatagata actttgatga ccttccagaa aatgcaaaga
    13921 aatatatagc aaaaatagaa gaactagttg gagtaagtgt agatatggtt tctgttggtc
    13981 caaatagagc tcaaactata ataagaagaa atatatttgc ttaatatttt atcaattaaa
    14041 taatttaaat agtaaatagt tgaagaagag tatctcaaga taatgtttat agttattaga
    14101 gatgctcttt tatgttttac ttacattgat tttttattaa gttaaaacta ctgatacaca
    14161 ttctaactga aattaaagtt gaatttgtgt agatacatat aggtttttta aaaagaattt
    14221 tgttgaaaat aaatagctaa agatgatttt aattatatgc taaaaaaagt aaaaaaaact
    14281 attgactaaa aattgtatag acgctataat ataacttgtg agtgaaagaa acgacgaaaa
    14341 aagtcgaaaa aatgtggtct attagctcag tcggtagagc acctgacttt taatcagggt
    14401 gtcccgcgtt cgagtcgcgg atagatcacc aaaatatggc tcgttggtca agaggttaag
    14461 acaccgccct ttcacggcgg taacaggggt tcaattcccc tacgagtcac cagtatggga
    14521 ctatagctca gctgggagag cacctgcctt acaagcaggg ggtcacaggt tcgagccctg
    14581 ttagtcccac caaattttga attagagata aatactagat tatatgctgg tgtggctcaa
    14641 cggtagagca gctgacttgt aatcagcagg ttgtaggttc gattcctatc accagctcca
    14701 caaagtacat aaattcaaat taatataatt ttctatgtta caggatactt ttaagtatct
    14761 ttttttaatt tcatttttta ttatattaaa aatgaaattt aacatacaac aaaattttga
    14821 attacttgca atgtgatagt tttaaatata aaatttagaa tcattctagt atagtattat
    14881 gtaatatagc aaatttaatt tatggtatca ataaatctag actactgaga atgtaaatga
    14941 tttgttaggt taatatacat tagatatgta aatagaaaaa taaaaagtat ggtttaattt
    15001 atgtggcata agttttaaac agagaagcaa gtttgctctt acctctctat ttaaaaataa
    15061 aaaaatgttt tttaagggct attttatttg aatctttccc atctcaaatc atctccagta
    15121 aattctaaaa tcctaaactt atctaagatt ctagagagag ttctttgagt gtaagtattt
    15181 cctatttgag acggtgttaa gttggttgaa ataatgattt ttttaccagc aacaagtctt
    15241 gtatttacaa tattgaagat ttctccacta gtaaaagaat tattaagttc agttccaagg
    15301 tcatctatta ttaataaatc acaatcaaac aagtttttat aattatcttc acttatttgg
    15361 ttatttgtgt ctcttctaaa tttataatct tcaagaatat ctaaaattct aaaagaagtt
    15421 tgatagatta caacattgcc tttatctaac aattcctttg caatacaatt gcacatatag
    15481 gtctttccta gtccagtaga accataaaac agtagatttt cactattatc tttcttaaaa
    15541 tcatgaacaa aattttcgca gatacttaaa ttatttagca tattttctct aggagatatt
    15601 tctccatcag agcctttctt aggagaaaat atattcaagt taaaattcga aaaattttcc
    15661 tgacttaaaa tcctatctaa attagacatt ttataagctt catttacaat ttcttgtttg
    15721 aggcaagaac atttttctcc attaggtaaa aatcctttat ctttacaaga aatacattga
    15781 aactttattt ccaagtaatc aagaggtatg ttactttcag ctaaaagtct ttctttcttt
    15841 acatttaaag actctatatt ctctttagtc ttctttacaa tttcatcttt agatttggga
    15901 tttaaaagta ctattttagc taaactgaga cctattttag aaatttcatc atctatagat
    15961 tttatctcag ggattctatt atatacctca ttttttctat gttctaataa gagttcattg
    16021 tcatctcttc tcttagcata tttagcaagt atttttctta ttttatcctc attcatttaa
    16081 tcacctactt aaacttttct ttttgacttt ttttaataat ctcatcgagc tcatcagagg
    16141 tatactgagt gaatgtttcg ttgaaattat gaaacttagt tttcttgtaa gtattgttgt
    16201 tttgagtagt attaatctgc ttcttattaa tattctcctt aactatacgt tcttcctctt
    16261 tttgctttaa atcatttaaa ttttttatat ttttttcatt ccaattttta attataccat
    16321 ttatataaga tatacttggg tttgaaatat tcttagattt agaacatgca tttattatca
    16381 aatctatatc catattaaat ttatcaaacc aggtgtccat aagttctttt tctgattttg
    16441 atggttgtct tgaaaatccc aattcattga aaatcgtttt atataaaata tatcgttcac
    16501 ttcttaccaa aaagctttct tcaacatctt ttggtgtata taaatttgaa tcataccaat
    16561 ttctaataat gccttcaata tatttaactg gctttgatgt accagtttta tctttgacgt
    16621 attcatatgc gcaaagtatc atatcggggc tcatattgta tttattcatt atatccataa
    16681 tactcatctt ttcacttggg tcgagatatc gcccaactat tttatttata gaattaaaca
    16741 tttttctaat acttggattt tcagaagtag atacaacctt atctgtatta gattttatag
    16801 atgaattatt gcttaatata ttctcaatat agaaattttt taaatctaaa aattctattg
    16861 aataattaaa attatcatat tctccattgt catgtatttt tataatcttc ttttcttccc
    16921 agaatttcca agcagataac acatcagaca atggtatatt taggtttttg gctattgaat
    16981 tattgtcgaa cttaggatta gaagtaatat cacatgcttg tctatatcct aaaagataaa
    17041 cttttacgta taacccatcc gccattggca taaatatatc tataaagata ttgggaatag
    17101 tgatttcacc taaatctatt tcattgcttt ctttaaaaaa cattttatca cctcagatta
    17161 aattatatca ttttttatat tatatttgtc tgtaaacaaa taaaaggtca caagattagc
    17221 atgtaaatat tttctaaaag tattcatttt gaaaaggagg agatattttg gttataaaat
    17281 tcaaccaaaa agtaagaaga acattatcag tagcgattct atttatggta ttaataatag
    17341 gttgctttat agaaaaggat gacagtttag aaacatacaa ggaaaaaact aattcaaaaa
    17401 taaatcaata taatatggat gttatatttg atgatgagac taagaggtta atgtgcaatc
    17461 agaatgttga ctacataaat aatacaaaat caaacataga taaaatatat ttccatatat
    17521 atccaaatgc tttttccaag aaagattttg ctccttttga aaaaagtgaa atgacaaggg
    17581 cttatcctaa tggatttaac gaaggatata tagatataaa gaatatatta aataataata
    17641 gtaaaatgga gtataaaatt aaaggagaca aaaatgacat attagaaata gaattaggga
    17701 aagagttaaa accaaatgaa agaatgtcat tagatataaa atataacgta aaaataccaa
    17761 attcagttgg aagatttgga tatggagaaa atactataaa tgttactaat tggtttccta
    17821 tagcttgtgt aaatgatgaa aggggatgga atttaaagag ttatgaaact attggcgacc
    17881 cattttatag tgagactagc gattttcatg tgaaactatt gattccaaat aaatataaaa
    17941 ttgctcatac aggaaaactt attgatgata aacatgataa taagaaaatg ctatatgaaa
    18001 tagaagctga aaacgttaga gattttgcat ttatactaag tagtaaattt gatgtaaata
    18061 aagtagattc aaagggaata gctatcaata catataattt aaataaaaaa ctttctaaaa
    18121 aagcaactga agtggcaaaa gattcaataa atatatttag tgaattattt ggaaagtatc
    18181 cttacaaaga attttctgtt gtagcaagtg acttttttat aggggggatg gaatacccta
    18241 tgcttgttat gatagaccaa agtttatata atgaaaaaaa tgaattttta ctagaatatg
    18301 ttattgccca tgagacagca catcaatggt ggtattctgc tgttggaaat gatgaaataa
    18361 gtgaaccctg gttagatgaa gcacttactg aatattctac aatagtttat tttgaacaaa
    18421 agtatggtaa agatatggca aacaagctta taaaaactat ggagatacag acaaaaagtt
    18481 atttgagcga aaatatattt aagccagcaa atgagtataa aaattctact gaatacagtt
    18541 tgaatgttta tacaaaaggc gcaatagcat ttaatgaaat aagaaaagaa gttggagata
    18601 aagtattctt tgagacttta aatgagtact ataataagta taagcataaa aatgctaatg
    18661 gaagagcttt tgtggaacta tggaatagta aaggtgtaga tataaataag attataagtg
    18721 aatgtaagta aattttgtgc agagtattaa attttattta atactctgtt tttttggtat
    18781 aatataattt ataaggcaga aaaagaggtg aaggaatgct aaagtattgt tcagtaggga
    18841 gtggtagtag tgggaattgc cactatatag ggtataaaaa tactaatata ctagtagatg
    18901 caggattaag tggcaagaga ataactacag gtcttaaaga cattgatgta gatgcagata
    18961 aattaaaagg aatatttata acacatgagc atgttgacca tataaaagga gcaggcatat
    19021 tatcaagaaa gtttgatata ccagtatttg ctaatattaa gacctggtgt tctatgaagg
    19081 acaaactagg tgacattaaa gataaaaata tgaaagtgtt tgaaaatgat aaaacctatt
    19141 cattaggtga tatcatagtg agaccttttt caatagaaca tgatgcatct gatccagttg
    19201 gatataattt ttatacagag agtgatgaaa aaatgtctat agctactgat ataggatgta
    19261 taactcaaaa tataaaaaaa catctatata aatctaaatt agtagtattg gaatcaaatt
    19321 atgaccctaa tatgcttatg atgggttcat atacatatgc tttaaagaaa agagtaatgt
    19381 caagcactgg tcatttatcc aatgaagatg cagctaattt ctgcgttgaa cttattaatg
    19441 aaggaacaga atctatatta ttagcgcatt taagtaaaga aaataatttt cctgagttag
    19501 catatgagac ttcaaaagga gtattagcat caaatgatat agtagttgga caagatgtaa
    19561 aactggatgt actatcacga aatgatgttt ctaatgttta tgagatgaag tagaggtgaa
    19621 taactttgct aaagagaaat tttgaaaatg ttgaggataa taagatttta tactctataa
    19681 agcagggtat gattttagca atacctgcta ttatgactgg ttcaactgct cttgtaattt
    19741 tgaatcttcc aataaaggcg tatcaagaat atttatcaag cttgtttaat ggagaggtta
    19801 caaatattct taactttata aatgattcta ctttaggaat tatttcattg ataatattat
    19861 taacagtaag ttatagttat gggaaaatac atgggagcaa gtatacagta cttgttccta
    19921 tagttgcaat gtgctctttt ttagtatttt cacatagtaa tgagcttaat tcttatatag
    19981 aaatatttaa aacaaagtgg ttatttacag ctataatcgt ttctatgaca tcttctgtat
    20041 tgtttgtaaa acttacagag tcttttgtta caagtgtaaa gtttcataca gaaggggctg
    20101 atgcagattt taatatggta gtttcagcta tagttccttt cataatagta gtatttttgt
    20161 tttctatttt tagagtgata atgataagtc taattggcag ttcaaacttt caagatatat
    20221 tttataattt attttcaaat gtgtttaata gaatgggaac aaaccttatg agtgctctgt
    20281 tgtttatttt tttaatgcac tttatgtggt tttttgggat tcatggtagt aacgtattag
    20341 ataccgttgc aaaaaattta tttgaaaata gtatggcaat taacataaat ttagttaata
    20401 ataatcaatt accaacagaa atttttacta agaccttttt tgatacaatg gttttattgg
    20461 gaggatgtgg ctctttacta tgcctagtaa ttgcaatttt tcttagtgaa aagcgtacaa
    20521 atgtaagaaa actagctaaa attgcctcaa taccagctct ttttaatata aatgagatgc
    20581 tagtttttgg aataccaatt gtttttaatt atattatgtt tgtacctttt gtaattactc
    20641 ctataatttt gaccattaca agttatgtgg ctatgtctac aggtattgta ccatgtacag
    20701 tttcagctgt agaatggacc tcacctatat ttttaagtgg atatatgtct acaggttcaa
    20761 taagaggtag tttgttgcaa ttatttaatc tatgtgttgg tgttatgatt tatattccat
    20821 ttataaagat gtcgcaaaat agatatacat atatgtttaa aaataatata gaaaatttaa
    20881 caaatttagt aaaaaggtat gagttaacag gtgaacagcc taatcttcta aaccataatg
    20941 gtaaaatagg ttcaatttca aaaatgttgg catcagactt gaagtttgct atgaaaagag
    21001 gagaaataga gcttttttat caaccacaag tcaattataa tggagatgtt gtgggagcag
    21061 aaggattact tagatggaaa cattcgatag gagggttcat ttatccaccg ctagttataa
    21121 ttttggctaa agaagaaggg ttcttagatg agcttggaga gtatattatt aataaagcat
    21181 gtattgattt aaagaagagt gaaaagttat tgagaaatcc tgttaagttc tctgtaaata
    21241 tatctccaga ccaacttgat gaccctaagt taccagagca aataaaaaat atcattagta
    21301 gtaatgatat aaatcctaat atgttaggta tagaaataac agagcaaatt gcattatcag
    21361 gttcacaaat aattattgaa cgtatcaatg cgatacataa cttgggaata aagttaatta
    21421 tggatgattt tgggatgggg cacagttctc ttatatatct tcaaaataat aactttgata
    21481 tagttaaatt ggatggttcg ttagttaaag agatacttac aaataaaaga agcagtgaaa
    21541 ttataatgac tattgtaaat ctttcaaaaa atttagattt tgatatagtt gttgagtatg
    21601 ttgaaaatat agaccaaagg gataagttat atggtttagg atgtgaaata tatcaaggat
    21661 attattatag caaagcaata ccttttgaag agtttgtaga atatgcaaat caagaaagag
    21721 agatataatt ttataagagc ttgtaaattc tagtaatgca tagatttgta ataaaattaa
    21781 gtttaggaga aatatatgaa tataagtata gtagtagtag gtaaaattaa agaaaaatat
    21841 ttaaaattag gtattgatga gttcaagaaa aggctttcta agtattgtaa gttagaaatt
    21901 atagagttgg acgatgaaaa agctccagaa aatttgagta ttaaggaaat ggaaattata
    21961 aaatacaaag aaggaaagaa aattttaggg aagataaagc ataatagtta tgttatagcc
    22021 ttggctattg atgggaaggg tttatcctct gaagaacttg ctaagactat gagtgattta
    22081 gcagttagag gtaatagtag tctctgtttt ataataggag gttctttagg gctttctgat
    22141 gaggttttag gaagggctga ttatagatta tctttttcta ggatgacttt tcctcatcaa
    22201 atgatgagat tgatattgct agaacaggtt tatagggctt ataggataaa taatggagaa
    22261 ccgtatcata aataaatgat acggtttttg ttggtttgta acttaataat attaaaataa
    22321 caaaaaagta ttgaaagtat attgcattta aggtagaatt aaataaaata ttataacatt
    22381 ataatgttta tatagttgtt aataaaaatt tggaggaaag tatatgataa aaataatgtt
    22441 ggcctgttca gcaggaatgt ctacaagttt gttagttact aaaatggaga gtgcagctaa
    22501 agaaaatggt atagagtcgc aaatttgggc aataccagaa tcaactatac aaaatgaaat
    22561 agaaaaatgt gatgtcttat tactaggacc acaagttaga tatgtattac ctaaggctca
    22621 agaaattgct aagccatata atataccagt agaggttata aatatgatgc actatggaac
    22681 agttaatgga gaagctgttt taaatagagc gatagaactt aacaacacta agtaatataa
    22741 attataatgt atttttaata tataaactgg gagaatcaaa gaatggatga aaagattata
    22801 gaaatatctt ttaatataat aggttatgca ggtgaaggta aagggttagc ttttgaagca
    22861 ataaaagagg ctaagaatgg aaatatagaa aaagcaaaag aattattaaa agagtctaag
    22921 gaagttataa ataaggcaca tagatatcaa acagaattaa tacaaaacga agctagtgga
    22981 aacaagactg aaataagtgt catacttata catgcacagg accacttaat gaatggtatg
    23041 aattttcaac agttagctga ggaaatcgtt gatttacatt tgaaattaca agataaataa
    23101 aaattaaaaa gattaaatta agagagttct taaagtatac attatgtgtt gcacactaat
    23161 tgttagatta ttgattcaat atttagtgag taatacatta ggcttatttt gaggactctt
    23221 tttatattta gtttaaagtt aatagatatt tattattaat aatataagat tatatgttat
    23281 aatattataa catataattt aagaggtgaa tcatgttaag aaatcaacca ttatatatac
    23341 aagttgtaga tatattaaag caaaagatat taaataacga atataaaatt gatgaaaata
    23401 ttccaacaga aagggagtta gagcaagagt tcggagtcag taaaattact attagaaaag
    23461 ctattgagat attagaatca gaagggtatg tagagaaaaa aagtggaaaa gggacaaaag
    23521 taataagtaa tagtcttttt aataaactat caaaagcaga gtctttttca agtatacttt
    23581 tatctaaagg tcataagtta tctaaagaga ctatttcaat tgaaaaaata ataaatgata
    23641 caaatccaca agtatttagt atatttggag aaaaatgtag caagattact agaatgtatt
    23701 acttagatag taagccatat atatttttta ctcattatat accttttgat gaaaatctaa
    23761 ctgttgaaag ttttaataat gaaagttctt tatatatgta cttatatagg agaaatataa
    23821 atataactag atttgaagat gaatttttta tagaagaatc agaactagag gtatcaaagg
    23881 ctttaaatct agctaaagac atattattag gaagaaaaag aaagacctat gatgagaatg
    23941 ataatatagt tgagttatca tttgcaagat ataatacaga agtacaaaac tatgtaatta
    24001 aatatgaaat ataataagaa tcctgagttt attaaaatga taatatgctc aggatttttg
    24061 tttatttgta aaaataaaaa atattataac attataatgt ttacaacaaa acattataat
    24121 gttataatat ttttaataaa taagtgatat tatcaattac ataaaaatta gtttgtgtta
    24181 tgaaatcgtt attgcattat aaataaagga taatatcact aatacagggg gataactatg
    24241 gaaaaattcg caagtttttt agagcgcaag attatgcctg tagcagcgaa gataagtaat
    24301 cagagacata tgagagctgt aagagcaggt ataatagcaa cattaccact aacaatagta
    24361 gggtcatttt tcacaatatt attaaacata cctattgatg cttatatgga ttttatagct
    24421 ccatttaaag caacattgga tataccattt agatatactg taggattttt atctctatat
    24481 gctacttttg gaatagcatc atcacttgct aaaagctacg atttagatac aacaggtgtt
    24541 ggtatgcttg cagttatggc gtttttagtt tctacaatag ttccaactca agtagttgaa
    24601 ccagtaggtg gagttataga agctggtaga tggatggata tggcaaaact taattctcca
    24661 tcactatttg gagctatcgt aacatcatta attacagttg aaatatatcg ttttatgaag
    24721 gaaaagaata ttacaattaa gatgccagca ggagttccag tagaggtttc aaattcattt
    24781 actgcattat taccgacagc agtaatctta atgttattct gggtaataag acatatttta
    24841 ggatttgata taagtgctgc actaagtgct atgttatcac ctttaaaagg attcttatca
    24901 ggaaatagtt taggtggtgg gctactgaca gtattcctaa taactttatt ctgggtactt
    24961 ggaatacatg gtccagctat aatgggacca gtaataagac ctatatggga catgtctata
    25021 gcagaaaata taactgcatt cacagaagga gtatcagctc atgcaatgcc aaacatattt
    25081 acagaacaat ttttacagtg gtatgtatgg atagggggag caggggctac tcttgcatta
    25141 gttgtaatgt ttatgttttc taagtcacaa tatctaaaaa gtttaggtag attaggattt
    25201 ttaccagggt tattcaacat aaatgaacct ataatatttg gagcaccaat agttatgaat
    25261 ccaatactag ggataccatt tataattgga cctataataa tgacaattct atcatatgtt
    25321 cttactatta caggggttat acctatgatg gtagcaagat taccatttgc aatgccagca
    25381 ccaatagcag cagtaatgag tactaactgg agtatactag caggtatctt agtattaatt
    25441 aactttgtga tatcatttgc tgtttattat ccattcttca aagtttttga aaaacaacaa
    25501 ttacaaagag aacaagaaga aatgagtgaa aatttataaa aatacataga tatagaagtt
    25561 ttgcactata taaaatatag aaaaagctta taagttatta agggtgaagg taggaggaat
    25621 gacctttgcc tttaatttta tatattttaa gtaatgtaat ttgaggagga gaaagatgga
    25681 aagaagatta ggaatatcag tatatccaga gcatagtaac atggaagatg ataaggcata
    25741 tataaaaatg gcttcaaaat acggatttaa aagaatattt atgtgcatgc tttcaattac
    25801 aagacctaag gaagaagtta aaaaacattt taaagagatt ataacctatg caagagaatt
    25861 aggatttgaa gtaatccttg acatagctcc aaacatattt gatacattac aaatatctta
    25921 tgatgattta acattcttta gtgagttaaa tgctagtgga ataaggcttg actatggata
    25981 tgatggaagt aaagaagcta tgatttcttt caatccatat gatttaaaag tggaactaaa
    26041 tatgagtaat gatgtagcat acttggataa tataattact tacaagccaa atgtaaataa
    26101 tatatatggt tgtcacaatt tttatcctca aaggggtaca ggattgccat atgatttttt
    26161 tgtaagatgt tcaaatagat ttaaaaagta tggaataaga actgctgctt ttgttaattc
    26221 aaaatacgca acaatcggac cttggaatat aaatgatggg ctgtgtacgc ttgaaatgca
    26281 tagaaactta gatattgcta catcaactaa gcacttattc gcaacaggat tgattgacga
    26341 tgtaattgta ggaaattcat atgcaagtga agatgaatta aaaagaatgt ctgaagtaaa
    26401 taaatatcaa gttgtattta atgtagtagc tgaggaaaaa acttctgaag tagaaaagat
    26461 aataatggta aatgaacaac actttagaag aggagatata actaatcagg tagcaaggtc
    26521 tacagaagta agaaaaaaat attctaaaga aaatttccct aaacatgata ataagaacat
    26581 ctttaaaaga ggagatgtag tgattggtaa tgatgacttt ggaaagtata aaggggaatt
    26641 acagttgata cttcaagatt gtacagatga aagaaaaaat aaggtaggta gaataaagga
    26701 agacgaaata atacttttag actttattga accttggagt aaatttaaga ttattttaga
    26761 gggagagtaa agtgaagata gacatattaa taaaatgtgg aaaaacagta gataaaagta
    26821 taattgatat agctatatta gacgataaaa taatagaggt aaaaaatcag atagatgctt
    26881 ctaagtatca ggcaaaacaa attataaact tagatggaga gaagtttatt agtgcagggt
    26941 ggatagatat acatacgcac tgctacgaaa gactagacct ttataaggat tatccagatg
    27001 aggttggaat aaaaagtgga gttactactg tagtagatgc agggacaaca ggagctttag
    27061 atataggtaa attttatgaa gatactaaaa aatataagac taatgtatat gccttaataa
    27121 atattgctaa gcagggaata acatctcaag atgaactttc aaatatgatg aatattgatg
    27181 aaaatgaact taaaaatgca gttagaaaat acaaagattt tgttgttgga atcaaagcta
    27241 gaatgagtaa aagtgttgta atttcaaatg atgttgaacc tttaaaagta gctaaaagaa
    27301 taaaaaatga acttaatcta cctatgatgg ttcactttgg gagttctcct ccaacgatag
    27361 aggatatatt tgactatatg gaaaaaggag atatattaac tcatatatat aatggaaagc
    27421 caaatggaat acttagaggc aatgacgtta aaaaggaaat catagaagct agagaaagag
    27481 gtataattct tgatgtaggt catggaaccg aaagttttag tatggacgtt gcaatgaaat
    27541 ctaaggacgc aggaattttt ccagacacta taagtacaga tatatacatt aaaaatagaa
    27601 taaatggtcc agtgtataat ctgtctacaa caatggaaaa gtttatatat atgggttatt
    27661 cacttgaaga tataatagat aaagttacta aaaatgcagc agatgcaata agtttaaaaa
    27721 ataaaggttt aataaaagaa ggatatgatg ctgatttaac tatatttgat atggttaatg
    27781 aagaaaaaga acttcaagat tctttgaata agtcagtaat cacaagtacg tctataaaac
    27841 caagagcagt agtagtcaat ggagaatatt taaatataga caagtctgta gaagagaatg
    27901 agtagaaggg agaaactatt gtatgagtag tatatttgaa aaatactctg taagacgtgt
    27961 tataaatgct tcaggtaaaa tgactatact aggagtatca acagtagata aggaaattat
    28021 tgaagcaata ggtgaagggt gtaaaaattt ctttataata gaagaacttg taaataaaac
    28081 aggtgaatat atagcaaatt tattagggtg tgaaagtgca ctaatagttt ctagtgcatc
    28141 tgctgggatt gcacagagtg tgggagcaat gatagcaaaa aatgatatga gtctagttta
    28201 caatgtaaat aatcctaaga aacaagttaa aagggaaata ataattccaa agggacataa
    28261 tgttgattat ggagttccag tagaagtaat gataagactt ggaggaggag aagttgttga
    28321 ggccggttat gctaatatgt gtaacaagga acatataata agagagataa atgaaaatac
    28381 agcagcaatt atgtatatta aatctcatca ttgtgttcaa aagagtatgc taagtgtatt
    28441 agatgcagta caagttgcta aagaatataa tttacctttg atagtagatg cagcagcaga
    28501 agaagattta aatgtatatt atgaattggg agcagaccta ataatataca gtggtgctaa
    28561 ggctatagag ggccctagct cagggttagt aataggtaag aaaaaataca tagataatgt
    28621 aaagcttcaa tcaaagggta taggaagagc tatgaaaata ggaaaagaaa atattgtagg
    28681 tctaacatgt gcaattgaga gatatataag taatgataaa gtaactttaa aagaaatgga
    28741 ggacaaacta aatccattta tagataagat aaatactcta aagggagtga gtgctagtat
    28801 aactagagat tcagcaggta gagaaatact tcgtggagaa attgacttta atgaagatat
    28861 aataaataag tcaacacaac aaattataaa tgaacttaga agtggagaga tagccatata
    28921 cactagggat tataaggcta atgaaggaaa ggttgagatt gatataagaa gtgtaattgg
    28981 aagtgattta gatataatct ataatagaat aaaaaatata gtagaaagtt agagtgtaga
    29041 gtaagtgaca ttataatgat ttaataaatt gaattttacc aatggaggag ataaatatga
    29101 attgtatacc acaatattat aaaaatagag tttgtttaaa tgttcttgca ggaagcataa
    29161 gaaatgctaa agaagtatat aatgcttgtg aaggacatgt actagtagga gtattatcga
    29221 aaaactatag tactgtagaa tcagctattg aagatatgaa aaaatactca aaggaaatag
    29281 ataatgcaat atctgtgggg cttggagctg gagacccaaa tcagtcaaat atggtatctc
    29341 agatttctaa agagcttcag ccacaacata taaatcaagt gtttacaggt gtggctacat
    29401 ctagagcatt gcttggtcaa aatgatagta taataaatgg attagtttct ccaactggta
    29461 aaccaggatt tgtaaaaata tccacaggtc cattaagttc aaaagaaaaa gatggtatag
    29521 ttcctataga aacagcaata gccatgatga aagatatggg gggaagctca attaagtttt
    29581 ttccaatggg agggcttaaa acaaaggatg aatatgtaaa agttgcaaaa gcttgtgcaa
    29641 agcataattt ttatctagaa ccaacaggag gtatagactt agataacttt aaagagatag
    29701 ttcaaattgc acttgatgct ggtgtagaaa aagttatacc tcatgtttat acatcaataa
    29761 ttgataaaga aacaggtgat acaaaagtgg aagatataga agtactttat aaaataatga
    29821 aagaattatt gggataaatt tatgaatata ttagcttttg gtgaaataat gatgagactt
    29881 tcagtacctg actacaagtt cttaacacaa accaatgaac taaattatgt aattacaggt
    29941 acgggattaa atatattatc tgggcttaaa aactttggat acaataccta catgttgaca
    30001 aagttgccaa ataataatgt aggtaaagca tcaagtgcaa atataagaaa gcttggagta
    30061 aaagatgact ttatcacata tggagggaat catatagggg tgtactttct tgaaagtggg
    30121 tatggagaaa gaccctcaga agtgacttat ttaaataggt taaatagttc attctgtgag
    30181 agtaaattac aagattatga tattaataaa tgtctagaag gaatggatgt tgttcatatt
    30241 tgtggtattg ctatgcaact tacagattca gttaaagaga ttgctttaga gctagctaga
    30301 aagtcttatg aaaaggggat aaaagtattc tttgatttta attttagaca atcactaaac
    30361 ccagaaaaga cttatgatga tttagtaaat gattataaga aaatgctacc atattgccac
    30421 ggattattcg ctagttatag agatataaat aagatactta atatagataa tctagatata
    30481 aaatcgagtt cagaattgat taaaaaagag tatgatatag acattgtagc tggaactatg
    30541 agaagatttg atgatatgga caatagattt ataaagggat atatatcaaa tgatgatggt
    30601 tattttgaaa gtaaggagta taagttagaa atatatgaca gggttggtgc cggagatgga
    30661 tatgtagcag gaattatata ttcatatttg gaaaatatga gtaatgaaga tatagttgag
    30721 tttggggttg ctagttcagt gttagctcat acaacttatg gggataattc tttagtaagc
    30781 acaagtcaag tacttagact catgaacaat gaaaaaatag atatattgag ataacacctt
    30841 tatgtagaat aatatcttta ttttttcaga gtcacctttg acttgattat atttgttaaa
    30901 cagagttctt tttagtgatt atgaatatta aattaaaagt taaataataa aatagtagaa
    30961 aatagcatta accatagaaa aaatggttaa tgctattttt atagttaata ttgattaatt
    31021 ttatattata gaaatgaatt tttctaatgc tggatttgta ttttttttat tccatacaac
    31081 tacaacttca attctatttt ctacttcttt tataggtact attgcaatat cacgttctat
    31141 cattgtatct gtaatgcaaa atgaaggtaa aatggatatc cccatgttag aagctactaa
    31201 tgattcaata gtattaagat tatttgaacg ttgtaccaca ttaggcacaa accctgcctc
    31261 acgatatcta ttttgtatca tagatttttg tccatactca tctcctgttt cctttacaaa
    31321 aataaattta tcatgttgta attcaattaa atcgatagaa tcctcattgt aaagtggatg
    31381 accaggaggc aaaagagcta ccaaagaata tctttctaca gttttatatt caaaatcttt
    31441 taatggttga ttttctggat taataacaaa agcaaggtcc atagaattat tttttactaa
    31501 gtttaaaagt tcggttatct cattagtaca aatatccata gatatattag ggtatgtatt
    31561 agaaaattct tttaaatatt gttgaagttt gtttttttcg tatcctatta aggttccaat
    31621 gctcaaagtt ccaaaaaaac catgtgcaaa ttgttgtgtt tttgcaatag catcattgat
    31681 attttttatt atagtacgtg cctcagataa aaaaactttt ccagctggtg ttagttcaac
    31741 atgacgttta cttcttgtaa atagagttac atttatttgt tcttctaaag cttttatttg
    31801 ctgtgtaaca gcagtttggg aaatataaaa tttttctgct gttttggtaa aacttagatt
    31861 ttctgctaca gatataaaat attctaattg tttagtattc aaataatcac ccctatttaa
    31921 acttgattgt ttctaaacct tatttatata taatta
//
LOCUS       DAEPXF010000023        31675 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain HB27
            SAMN13376389-rid12479423.denovo.024, whole genome shotgun sequence.
ACCESSION   DAEPXF010000023 DAEPXF010000000
VERSION     DAEPXF010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13376389
            Sequence Read Archive: SRR10518432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 31675)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31675)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001069624.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001069624.1
            Genome Coverage       :: 315x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/15/2021 01:54:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,165
            CDSs (total)                      :: 4,076
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 89
            rRNAs                             :: 10, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 10 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 19 of 69
            Pseudo Genes (incomplete)         :: 44 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 10 of 69
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31675
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13376389-rid12479423.denovo.024"
                     /strain="HB27"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="China: Hebei"
                     /collection_date="2017"
     gene            complement(412..1239)
                     /locus_tag="KRL67_000980"
     CDS             complement(412..1239)
                     /locus_tag="KRL67_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIM24 family protein"
                     /protein_id="HBH1521881.1"
                     /translation="MDLSKKVKILENASDGKSEFEILEFEKLEGASSPLMAMGLYFAR
                     EAGLKARQIKIKLNNSSIKTEAGALYYYKGNISSETKIGGVGGLFKKGISGALTNESM
                     MKPVYKGTGEVYLEPSFRHYLFMSLDNDSIIVDKGLFYCCSDTIEVKAFTQKNFSSAL
                     LGGEGFFQIELSGTGVIVLECVVPQSEIVEYELKNGEELKVDGNFAIARTSGVNFSVT
                     KSDKSLIGSALNGEGFLNTFTGNGTVWLAPTQVMYEKLSFGTMPTNDNMNNKVSKQK"
     gene            1817..2272
                     /gene="smpB"
                     /locus_tag="KRL67_000981"
     CDS             1817..2272
                     /gene="smpB"
                     /locus_tag="KRL67_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HBH1521882.1"
                     /translation="MAGKKILATNKRARHEYFIEETYECGIELKGTEVKSIRQGKVNL
                     SDGFASVDNSEVFLKQVHISPYEQGNIFNVDSLRVRKLLLHKHEIRKLIGATTIKGYS
                     LIPLSLYLKNGKVKVEIALAKGKKLYDKRQDMAKKDAQRRIEREMTGKY"
     gene            2430..2779
                     /gene="ssrA"
                     /locus_tag="KRL67_000982"
     tmRNA           2430..2779
                     /gene="ssrA"
                     /locus_tag="KRL67_000982"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(2923..4101)
                     /locus_tag="KRL67_000983"
     CDS             complement(2923..4101)
                     /locus_tag="KRL67_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HBH1521883.1"
                     /translation="MATKRANGEGSIVKNIRNGVQIGWRASISIGRDEKGKLIRKQFT
                     GKTQKEVKEKLDIYRTKMLLGSIVSADKITFEDWFYTWLFDYRAKDLKPKSFERYEGI
                     YRNYIKDTELGKIKLIDLRATHIQRYYNKLMDVYNKPASTIITLNTRLKPCLTEAEKQ
                     GYIQKNYCKMVTLPSDNKIKEVQILSITDQKKFIDSIDGDKFEILFLLALSTGLRLGE
                     LLGLKWSDISFDDESLTVNRTLQRVTEIKKDGTREKKIIEQYPKTKNSIRTVPIPRNI
                     LVKLKRHKIQQTEQRLLLGDAYINNNYVICNDTGLALNNNRPGKILDSLLKKLNIPKI
                     KFHALRHTYATRLFEAGVPPKTVQTLMGHYDISITMDIYTHVMHNTKQDAVDKINDIF
                     "
     gene            complement(4149..4370)
                     /locus_tag="KRL67_000984"
     CDS             complement(4149..4370)
                     /locus_tag="KRL67_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBH1521884.1"
                     /translation="MLKQARKNKNLTQKQLSKIANISQSYISRLEQDIFINSPTIRQI
                     ISLSKALDISAYKLSNYFINKENAYNKKR"
     gene            5124..6284
                     /locus_tag="KRL67_000985"
     CDS             5124..6284
                     /locus_tag="KRL67_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction enzyme HsdR N-terminal
                     domain-containing protein"
                     /protein_id="HBH1521885.1"
                     /translation="MDFSDKIKQFSKRIEVIKENLTTEEATKTALIMPFFQILEYDVF
                     NPLEFIPEYVADVGVKRGEKVDYAILDDGKPIILIEAKNIKDKLTKHDAQLFRYFTAT
                     EARFAILTNGIIYKFFTDLEEKNKMDEKPFMTINLLDIDENKISHLKKFAKTTFDVDV
                     VFNIASESKYTNLIKEQLNEQLESPSDEFVRFIINDFYNGVKTQNVVEKFRPIVKRSI
                     LQFATDFMNEKLKSLLESNDDEDEKEKKKIIDKKAKEETERKNKEKTEKEKMRKVEEC
                     QKEETKIEVVKEDQQQISAEEKEAFDIVQDILGDLSCKEDITYKKEKEWLNILYKADS
                     NLWICNIKLTSSDKLISLPNQNNEPVEYRMDEVIDIRELKDELIFVLSKYTN"
     gene            complement(6331..6678)
                     /locus_tag="KRL67_000986"
     CDS             complement(6331..6678)
                     /locus_tag="KRL67_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBH1521886.1"
                     /translation="MSKFYERLAQLRKEHNLTQMDLAKIINKQRSTIAGYETDRKQPD
                     FDTLCTLANYFEVSIDYLLGFTEDKKTYDSTLEDLEIDDDVKKITDIIFKLDEEDREA
                     VFKMLNALTKKYK"
     gene            6843..7061
                     /locus_tag="KRL67_000987"
     CDS             6843..7061
                     /locus_tag="KRL67_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBH1521887.1"
                     /translation="MRSKLKQLRIKKNLTHKVMAKELGISRSYYTSIEMGNKTPSLKT
                     SMKIKSFLKYYKDDIFLDDKVSKRNNLD"
     gene            7089..7526
                     /locus_tag="KRL67_000988"
     CDS             7089..7526
                     /locus_tag="KRL67_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBH1521888.1"
                     /translation="MLQQCQNIYQISRENARLTQEKASELLGISVESIRAYENDKRIP
                     NNTVVAKMVSIYNNKLLGYEHVRRNTEAGIMFLPELKIKNLSSIALKLHKEIKDYLKK
                     EDDFIDIVEDDVIDEEEEEVWNDVMNELDGIYEAIFALKFAKK"
     gene            7538..7768
                     /locus_tag="KRL67_000989"
     CDS             7538..7768
                     /locus_tag="KRL67_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBH1521889.1"
                     /translation="MEVNENMQTKVVNLKTKKSRKIMTVKEFSKEYEIGINNAYEMTH
                     IKGFPAFTIGRKICILRDKVDEWFLDNIGNKF"
     gene            7792..8556
                     /locus_tag="KRL67_000990"
     CDS             7792..8556
                     /locus_tag="KRL67_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH1521890.1"
                     /translation="MGELGWIKLHRALKDNPTWKACKAERKVIMITMLLLASHKETVI
                     TLNTGKKVKILPGQFITSRSSLAEKCGKDVSEQMVRSALQFFKKTNFSTNESTNGYTL
                     VTIINWGLYQDVESKQPTSQPVVNQGSTTIKNVKNVKNKKLYVETSNEYRLAEFLFKH
                     IRKNNPNAKTPNLQSWAKDFDYILRLDKRNLEEVQELIRFCQNDSFWCTNILSASKFR
                     KQYDQLYLKYKNEKNPLNKKAKEDGVYNPYDTDLMI"
     gene            8571..9905
                     /locus_tag="KRL67_000991"
     CDS             8571..9905
                     /locus_tag="KRL67_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase"
                     /protein_id="HBH1521891.1"
                     /translation="MSSVNDLVKSTEAEQSVLGSILLDATAIREIEPILTEDMFYHEE
                     HKIIFDSMKKIYKRNEPIDLITLTEEVKHKGHLNRVGGISYITSLSNVVPSLSNALYH
                     ANIVLDKYKNRNIIDKFNKFKLGEIDDNTLIYQLEQDINKIDKKLIKKDTSISAITTR
                     AFDYISDGVEEGIEIGIEVLDETIGGLYQGELTTIAAKSGGGKTALALQILRNVIFQG
                     KKVLFISGEMSDIQILFRNISSLTGIPIVKMKNRDLNEKEMIDYINALGILNQDEILD
                     INDTVNTISGIKREIKRLKPDLVIVDYLQILDYEGKEVGEAKFAELSREMKKITLDFN
                     IPVIQLAQLNDEMRDERPKGDRPMRSSKQIYMDSNNVVYLHRPTEKEALEYCKLIGIE
                     DIEALRKREKSLGVKLTEIIVAKNRDGEARYTPYWYVGCRLTFKKCTMAKII"
     gene            9920..10549
                     /locus_tag="KRL67_000992"
     CDS             9920..10549
                     /locus_tag="KRL67_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HBH1521892.1"
                     /translation="MYEVELMELIDKSKDGNESAFESLLKIFDKDIQINANKYVNLLQ
                     GGFDYNDLIQVARIALWKALSNYSHTKGKLHPKAYFKKAIKLSMLSLLNSGNRKKHRT
                     LNKSISLNNFISKDEQIELITLIDNNEDDIESQVISKEITRELKNKVKKDIVYQTDMQ
                     RKVISLRVDGYEGREIERILGISLKSINCHLYRARKIIKQNLGSVNYEA"
     gene            10539..10796
                     /locus_tag="KRL67_000993"
     CDS             10539..10796
                     /locus_tag="KRL67_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH1521893.1"
                     /translation="MKLKKLISKLSRLEDVEIVFHINVKNIPIISSIKLSSIIMNHDE
                     VEIYGLNNFKLYIPVDEIIDIVEYPESLILYSLSFQVIFRW"
     gene            10810..11103
                     /locus_tag="KRL67_000994"
     CDS             10810..11103
                     /locus_tag="KRL67_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH1521894.1"
                     /translation="MTLDLNIKELYELGFSEEFIALAKAHLLDDFIFNDVPKLSYTNV
                     GKMYDIKKYEIFIVVNETLIYILQTQYKEEFLPVEEQILSFGTVKKMLEYVCK"
     gene            11289..11801
                     /locus_tag="KRL67_000995"
     CDS             11289..11801
                     /locus_tag="KRL67_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HBH1521895.1"
                     /translation="MWIYHKRWKTKRFHKIIIEWSGVMSDLKSESFKKAENKLYSYNR
                     VKTEIKNLNLEIEEKENLYMGCCSIGYGEKSGTTYNISNLVESEVLKKEKEVEKIKRY
                     IKVKEIFIEKIDNAILLLNEDEIALIKHRYFSNKKNSWNYVANKIGFSTARCKQMRIE
                     IINKLKDLLS"
     gene            12173..13000
                     /locus_tag="KRL67_000996"
     CDS             12173..13000
                     /locus_tag="KRL67_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH1521896.1"
                     /translation="MLYDITSINGAYKFLSEYLEVDRDYIDLYIRRNPSKYKFKKFIE
                     EIEFIKKIKFNNLKLEDIDIILIHITTNDDDCNSIKKYGLINLIQAITLETPLSKYLK
                     SFNIVFDVYNYLIFIDEQVFSIRDYKESKEVIRLLYLKLYDDYNINAFFYVRDFKSYG
                     GRVHEKPEILCNLSKAFNNKNISEKWKNNIHNKCYSVKFKVRLEELEWYTFYDNIEEY
                     KKDYTKKDLIRNELVENALSVIHEDYFKKDSKSELIAYLKREVLIPYSNIIEIEEIK"
     gene            13287..13886
                     /locus_tag="KRL67_000997"
     CDS             13287..13886
                     /locus_tag="KRL67_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HBH1521897.1"
                     /translation="MKRPAKPIKKQQDVLDIQDYLRYKSYRNYVIFILGITTGYRAGD
                     LVKLKVREVKEALKRQEFTIWEGKKVNSKNIREKNRKPRTVEIRPKVTHILKKYIKNK
                     KDYEYMFPSRKKDYPYIGVAAISKILKEAGEYFGLYDITAHSMRKTYAYKIYIDSGKD
                     IVAVKELLGHSSIEETKAYLGLDKELYHHYSKSLDDFVR"
     gene            14083..14376
                     /locus_tag="KRL67_000998"
     CDS             14083..14376
                     /locus_tag="KRL67_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH1521898.1"
                     /translation="MKEIELSNKEIELIISVLDSKISSNLDKVFGDVQYSPTCKHRLR
                     KENSIIENLIDKLIDRLNGIEVEGKENVLRIDWNMEEFEGKLKEVIEEYLCNG"
     gene            14369..14662
                     /locus_tag="KRL67_000999"
     CDS             14369..14662
                     /locus_tag="KRL67_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016056424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HBH1521899.1"
                     /translation="MAREFSQSFYNSKAWKDCRQAIVKKYLGLCAECGKLGEEVHHIK
                     HLTPSNINDVDIALGEENLILLCKDCHSKKHKSKKDITRAGLKFNENGELISI"
     gene            14760..15149
                     /locus_tag="KRL67_001000"
     CDS             14760..15149
                     /locus_tag="KRL67_001000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH1521900.1"
                     /translation="MGGGLFKIIYEQLEKEKKIKQEVSRLKKNYKDLEKEKVKILDGL
                     VNEAAFLKISLEETREILTKEGLTEIFKQGKQEFERERLQVKIYLNFMKLYSNVMKQL
                     IDIIPSDMKQEEEDELIKFIKKGRLNK"
     gene            15146..16795
                     /locus_tag="KRL67_001001"
     CDS             15146..16795
                     /locus_tag="KRL67_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HBH1521901.1"
                     /translation="MTYIEEYYQKILNGEIVACSRIKQVYKKLVQDLYNPKGNWVFDE
                     ELANRPIEFIETFCKQAQGKLGEPLKLELFQKAKHQAVWGFVDKETRFRKYQEVLDIR
                     GRKNGKTTELAADELFMLIADNEGSPEVYNIATKYEQAQKGFKECYKMVQQSKILSKH
                     IKKRKSDLYFHANYGFLQALASNSNGLDGLNSHMVTIDELAAIKNRDIYDLMKQSMGA
                     RNQPLLNCITTNGFVREGIFDAQYEYACNVLDGKIKDDRFIAFIYELDDKDEWDREEC
                     WIKANPGLGTIKKLDFLRDCVNKAKADPNFKPTVMVKDFNMKENSATAWLRWDELNNE
                     TKFNADEMGFRYGIGCFDLAETTDLASAKVLLRKRYDDNIYVLSMYWIPFERLEQKTD
                     EDKIPYILWEKQGLLRICEGNKINPYDILRWFREIKEEYDIYIPWIGFDPWHVDSSLL
                     LAYENEFGKDSMIKVRQGAYTLSAPMKELRADLKANKVIYNNNSIDKWCLSNTEIKTD
                     INGNIQPIKGVDRRKRIDGAVSLIIGYVVLKEKMSEYENMI"
     gene            16811..17965
                     /locus_tag="KRL67_001002"
     CDS             16811..17965
                     /locus_tag="KRL67_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HBH1521902.1"
                     /translation="MNIFKSKKKNKEAPGKVAIELITDSGNGFYSWCGNLYRSDIIRS
                     IIRPKSKAVGKMTAKHIRSNETEFKTNPEIYIKFLLENPNPFMSGQILQEKMVTQLEL
                     NSNAFAVIIKDSYNIPIQIYPLNALNVEAIYEDEVLFLKFSLKNGKIVTYPYSNIIHL
                     RKDFNENDLFGTPPTKVLEPLMEVVNTTDQGVVKAIKNSNTIKWLLKFKTALRPDDIK
                     KEVKEFEKNYLQIDSDAGGAAATDSKYDAEQVKAESYVPNAAQMDKAIQRLYSFFNTN
                     EKIIQSKYTEDEWNAYYESEIEPVGLQLSNQYTEKLFTRKARSFGNEIIFEASNLQYA
                     SMSTKLNLVQMIDRGSLTPNEWRKIMNLSPIEGGNKPVRRLDTAVVEGGE"
     gene            17971..18708
                     /locus_tag="KRL67_001003"
     CDS             17971..18708
                     /locus_tag="KRL67_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease ClpP"
                     /protein_id="HBH1521903.1"
                     /translation="MANDNLNEFLKIKNSTETSSSLYFYGDIVCDEWDAWTEEDQYPL
                     SIKNFLATEQGKDLNIYINSGGGSVFAGMAIYNILKRHEGFKTVYVDGIAASIASVIA
                     LAGDKVVIPENAYFMIHKPWLGLFGAYNSDKLIKASQDLDRIEEGILNVYKDNLREGI
                     DIEEIKEKLREETWFTGKEASNYFNFEVDEKKEVAACVSDYFDKYNKMPNILKSKIDK
                     DSNKKENNNKKRVQLRLDLLKLGGLND"
     gene            18701..19969
                     /locus_tag="KRL67_001004"
     CDS             18701..19969
                     /locus_tag="KRL67_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HBH1521904.1"
                     /translation="MTREEYFKKRQEMIDEAQKLLDDEVGEEGAGEENTEEAEKIANR
                     IKALDEEYERNVKARANLKALQDNVKIDPTIFNLTNNKGKIEGIEDTTVKDKQEQYKN
                     AWAKDMLGKQLNSEEQEIFNSINSAYRAEVQTSENNTILIPKTVASGIWKEIGDMYPL
                     FGDASPTFVAGDLTIIAEEDGGDDAAWYDEETEVKEDGYKLKEITLRGCELAKDITVS
                     WKLKKMSIDEFVPYITSLLAEKMGAALAKAIVDGKGKPGESDSFKPQPLGIKTALSKE
                     ASKAQIIEYTDKIAYTDITKLMSVLKKWSNGACIYANSTTIWTQLAEILDTTGKPIFI
                     PDAANSDGVGRMFGRVVKMDDSMADGEILAGNIAKGYAININENVTLYTDEHVKSRKT
                     DYLTYSLVDGNVISNKAFGMIVKKTSAVAK"
     gene            19979..20266
                     /locus_tag="KRL67_001005"
     CDS             19979..20266
                     /locus_tag="KRL67_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016056381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage gp6-like head-tail connector protein"
                     /protein_id="HBH1521905.1"
                     /translation="MIVSLEEIKEYLRLEADYKEDDNLLLSFLKAAEEDLENRTGKVF
                     NENNKSELVGLYIKMYSAEQYEKRGATESNSEKVRFVLESIISQISICSRY"
     gene            20268..20606
                     /locus_tag="KRL67_001006"
     CDS             20268..20606
                     /locus_tag="KRL67_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HBH1521906.1"
                     /translation="MDVGKLTQRIKIQIYGEIENDIGEITKGWSTYKKLWANKSLLRN
                     SNNYVLDKENIEYSYRFKIRYRTDITEAMRIVCNDVVYDIKHVNNIKELNKYETNIDC
                     ILYKEGVYNE"
     gene            20599..21012
                     /locus_tag="KRL67_001007"
     CDS             20599..21012
                     /locus_tag="KRL67_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 gp10 family phage protein"
                     /protein_id="HBH1521907.1"
                     /translation="MSNTDFNMNGLDDYTNKLFKRIVKEYPKKAEKLMNTSLGKCKGE
                     AIARTPKSSKKPKKYKRAKHMKDNWKTKVQSKNGNCTGVLKNDSPHAHLIENGWATKN
                     GGYVEGKHMLQQTMEHQRVKIDKRIEKMVDETFNL"
     gene            21024..21440
                     /locus_tag="KRL67_001008"
     CDS             21024..21440
                     /locus_tag="KRL67_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH1521908.1"
                     /translation="MLKIVSVKKAIVEKLKSLNIKIVANEIRSGFEKPAFFIQIIPVE
                     MSSDPNFSNSTLLINIHYFSKEKTELENLKMIDKLNVLFQDCILEIDGGELTIEEKSV
                     EIYENVLQYKFNLQVVEIIEEDESKYELMKELEMNI"
     gene            21455..22522
                     /locus_tag="KRL67_001009"
     CDS             21455..22522
                     /locus_tag="KRL67_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath subtilisin-like
                     domain-containing protein"
                     /protein_id="HBH1521909.1"
                     /translation="MGLPSAIIEFQRRSKTVKFRSRRGVVALILKDSTAIKKSYSINF
                     LTDINETEFTKENYDYIKLAFLGKPSKVIIEVINDSVDSERSLDDALKALRENKFNYL
                     AIPFISEEVDKTKIVNWIKIARREKEIYKAVLPSVNNANEKAIINFTTAGIKVGEKSY
                     TTAEYTARLAGILAGISLSESCTYFALDEVTEIEPAENPDEAVEKGKLILINNNGIRI
                     ARGVNSLITLSKEDTEDLKKIKIVEAIDMIQDDILQTWNENYVGKVTNKYDNKILFLS
                     AVNNYFKELQRDEVLDNSQEAYVQIDIEAHKKYLKEKGIDYSEMTEQQIKEANTGSYV
                     FVEGNITITDAMEDLKFKIYM"
     gene            22535..22984
                     /locus_tag="KRL67_001010"
     CDS             22535..22984
                     /locus_tag="KRL67_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tube protein"
                     /protein_id="HBH1521910.1"
                     /translation="MSKENIVGSSQISGTWGKLWWDGTLIAEVLSFEAKVTANREEIQ
                     FGMSKDSKITSISGEGTVKLGKVYSRGKKKLLEAWKKGEDPRSTLTSKIKDPGTPGKQ
                     AETVTINNVWFNELALAQFEKGGKIEEELSFGFTPNDSDMMDEIEEI"
     gene            23033..23416
                     /locus_tag="KRL67_001011"
     CDS             23033..23416
                     /locus_tag="KRL67_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HBH1521911.1"
                     /translation="MENKKEMITIEDILRRKEYFAKKSEETKQLYIPSLGGNIEIAKP
                     DRELCIDVTEMENSIESDKYFVYEIVKKPNLKSEKLHTEFGCKDNPLDIVDVLFETGE
                     IADIAKIAASFAGFGVVEEVEDLKN"
     gene            23434..23607
                     /locus_tag="KRL67_001012"
     CDS             23434..23607
                     /locus_tag="KRL67_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH1521912.1"
                     /translation="MQMISHYLEKGVDLDKLTNLNIIERNFRIACMLYEEEEKIKLIS
                     ELIGAVFGGVKNG"
     gene            23600..25354
                     /locus_tag="KRL67_001013"
     CDS             23600..25354
                     /locus_tag="KRL67_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH1521913.1"
                     /translation="MARRHISAVISLKDNMSATMRGIRREQKQFQNEVRRTRNEMRSA
                     SRERMRIRMDATPAHRTIQNLRQKLAPLRTKLVKAVVIKDLATEKIERIKSNVKSFGK
                     FIARPAIKLKDETKGMIDKIKNRLTSLSTIVPVGAAIGAAGMAVKSGMELEQQQISMR
                     HFMGVGNKGKSSKELDGMSASYLKDLRNNANATPFETGEVISAGTRSLQIAGGNTKDA
                     MQMVKLAEDMAALNPGKSVGDAMEALADMNIGEMARLTEFGVKASSTDDPKEVQKKLE
                     TMYNGGANKLAESGSGLLSTIMGKLKSNIADIGLGMLEPLKPVMTGLIGFIDQASPKI
                     LEVGTKITAGIGMAIGWIQQQMPTLAPIFQTAFGAVSSIVSTVAPIIGQVIGALSPIF
                     MGLLSVASSVLAGIASAVKTVAPVVSSLISKLAPIFKNVGDTLKSMGKIFKNVFDSVM
                     KIVKKASDYIKPLVDGITGAAKGISDGVSWVAGKLAGNATGTKYWSGGLSVVGEHGPE
                     LVSMPRGSKVFTNAESKSMINKSIPNFRQVQGENTNYNITIPKIAETIVIREDADIER
                     ITSSLIKKIQMAKMGGVV"
     gene            25354..25989
                     /locus_tag="KRL67_001014"
     CDS             25354..25989
                     /locus_tag="KRL67_001014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016056389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HBH1521914.1"
                     /translation="MEMWLRQSNDAFRFPIFPASFEISGNINTSTTNVLRLGEVIICG
                     GTGLRTTEISSFFPSRQYHFCNYKDFPQPYDCVNKLKRWMEQGLILRYIITETDVNME
                     VIIESFKHGKQDGTNDVYFTLSLKEYKRIQIPSINSSDGKIEVVKNVPVTKGFETGKQ
                     KTHKVVKGDNLWSLSQKYYGNGDLYEKIVEANKELIKTADIIKEGWVLIIP"
     gene            26229..27746
                     /locus_tag="KRL67_001015"
     CDS             26229..27746
                     /locus_tag="KRL67_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HBH1521915.1"
                     /translation="MIINRSKDSSNNSISFVSKDMGFLLTQSEVSYNFKDKLVEDIAK
                     QVFNDNKLAVGNIPKTNVKYTKMFIGVTGYDTIMSAYTEASKTTKKKYMIESNLDKFN
                     VIEKGTITLNVMFEEGSNLINTSFSESMENVKNKVLVVDQYGNKISEKVDDKIFKDVG
                     VIMQKVIQQQENSTVDIESEFKGIEQTCSLKGYGDVTCITGRGVKVKDRYTGLVGLFY
                     IDTDKHNWDSNGNYEIDLDLNFQNIMDEKTAGQDEQKESSGLNGEGTLNGKEVKAEFT
                     AYYPSNNAMEGGYYQAMDGKRLVPSNNTCAAPSKLKFKTKIQAKCPGTKIDGKTYTVT
                     DRGGAIDLKNGVYRIDILMSSEKECNDFGRRKGTIIIGDGTGYTNATGKAKELISIAK
                     SKLGCKYVWGATGENTFDCSGFTQWCYKKIGISIPRTASAQSKAGKPVDLNDRSKWKA
                     GDLLCRVSGGSNNHVVMYIGNNQIIHSPQTGDVVKIQSVDSYRKGKAYTHVRRYL"
     gene            27762..28088
                     /locus_tag="KRL67_001016"
     CDS             27762..28088
                     /locus_tag="KRL67_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2577 family protein"
                     /protein_id="HBH1521916.1"
                     /translation="MANPINEFIGIIREEGKYHNQPSFFIGKIKSKLPDLKIEVNNII
                     LEKNDILIDSWMLDRQIEIFNTETNQEHKHEVKNPFIDKFELDDTVIMFKISDKFAVV
                     SKLVSL"
     gene            28088..28516
                     /locus_tag="KRL67_001017"
     CDS             28088..28516
                     /locus_tag="KRL67_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2634 domain-containing protein"
                     /protein_id="HBH1521917.1"
                     /translation="MSTIFPFIGVPEDYILPKTEELPIFREVAWNFEKDKPILENGDF
                     KIVEGNEAIKVWVYKCIKTNKYEHEIYSWGYGTELSELIGQKYSKGLTESEASRYIKE
                     ALLVNPYVLDVNISNTRFIDDLLSVDIVINTIYGEVEVNV"
     gene            28509..29564
                     /locus_tag="KRL67_001018"
     CDS             28509..29564
                     /locus_tag="KRL67_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HBH1521918.1"
                     /translation="MYSSQTYDVVKNRTLSNIDLDIYKGEGSFLSDMVSPVNSELAKF
                     YIELSYLHKKAFIEDNFDDFLDKRVNEFGVYRKLGTEATGEVIFEGKVGTTIQNGTII
                     SYNELLFVVIKDIVISSEIEQNTSPVQALEIGIRYNIPASTEFKLQDEINGITKIYND
                     LAFRGGTEIETDKELKERFYKIQKNQATSGNKAHYEAWALEVEGVYNAKIYPRWDGPG
                     TVKVLIFGENNQAVDSEVIERCREHIEEEMPIGPMLTVLTPSVLDISISASIKLETGY
                     TLDFVKESFLESINSYLINVNKEIIYTKVSAILASIEGIHDFSNLLLNNKAENIVFEE
                     DKVPSVTNLEFSEVVVQ"
     gene            29561..30178
                     /locus_tag="KRL67_001019"
     CDS             29561..30178
                     /locus_tag="KRL67_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2313 domain-containing protein"
                     /protein_id="HBH1521919.1"
                     /translation="MKLIDKLPSFYNNDITRKIQEAYDIELETLRETYDDTFDQFFVD
                     TATWGLDYWENILSIKNRFDLSIEDRRSNIKAKMRGKGTTTIEVIKAIGEAYTKTNVD
                     VEVFSNLFSFTLSFITNNCSYNTILELDKKIEEIKPCHLEHKFEMILFNQNDMFAGST
                     TNTGETVTIYPYSPTDITSFGELTITTGNDRSMEKVTLYPRQEVV"
     gene            30180..31181
                     /locus_tag="KRL67_001020"
     CDS             30180..31181
                     /locus_tag="KRL67_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015984856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBH1521920.1"
                     /translation="MAEQKYYTLLTTIGKASIANATALGNKVDLVKLQLGDGAGTEYN
                     PTEEQTSLKNVVWEGSVNNVKIDEENPNWIVIETVIPGSVGGFMIREVGIFDSKEQLI
                     AVSKYPETYKPTADSGSVKDLVIKIILVVSNTSSVNLKVDPTVILATLKDIQELDTKI
                     DTTKTELTSNIETAKTELNTRIDTENEKQNIKIDQLVAGGVNVSHTHIIEVADWILNN
                     ETNMYEVTINHPLLTKRILIALYDEIGEALTPNARAIDDNSILVRNEENIKMYVYLIN
                     GNAETHFINATVDDNRVSEMTTYSSKKIEDRLVNIEEKLSGNLSDIATSVNELITYC"
     gene            31198..>31675
                     /locus_tag="KRL67_001021"
     CDS             31198..>31675
                     /locus_tag="KRL67_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH1521921.1"
                     /translation="MATEWNFDFKAEAQPITLKAGKYKLECWGAHGKVWGGDSQSSGG
                     YSYGELTLKKETTLYVYTGATGSSNKYEKFTFNGGGLGVNNGGGGATDIRLVNGDWNN
                     EQGLLSRIIVAGGGGGAFSKTPAGKGGGFKGGNSTNDDNSSMLIVPGGTQYDGGRGY"
BASE COUNT        12606 a         3519 c         5883 g         9667 t
ORIGIN      
        1 atcaaataaa gttgaatctc ttgattacct tgattatttt atgaacttaa tatagatgta
       61 aactttacta ttaaaaaaat acgagatttt agataagtta atgctaattt aaataataag
      121 ataagaaata taatatgaca taagagctag gaataagata aatataatca aagaataagg
      181 catgagaata attaccaaga gaaatcatta ctttaatatg tttatgaatg ttagactatg
      241 ttcctaaatt gaattttata tatcaagtat aataacttta cagaatgtgg aaatttttat
      301 ttaaagaata cacataaaca tttccacact ctgattttat aataataatc taataaaatt
      361 tatagtgact ttataaactc aataatgatt taataaatat ttataaaaca actatttttg
      421 cttagaaact ttattattca tattatcatt agtaggcatt gttccaaaac ttagtttttc
      481 atacattact tgagttggag ctagccaaac tgtaccatta ccagtaaatg tatttaagaa
      541 accttcacca tttaaagcag aaccaattaa agatttatct gactttgtaa cagaaaaatt
      601 aacaccagat gttcttgcta ttgcaaagtt accatctacc tttaattctt caccgttttt
      661 caattcatac tctacaattt cactttgagg aacaacacat tccagaacga ttacaccagt
      721 accacttaat tctatttgga agaagccttc accaccaagt aaagcagatg agaagttctt
      781 ttgtgtaaat gcttttactt ctattgtatc agagcaacaa tagaataatc ctttatcaac
      841 aattatagaa tcattatcaa gactcataaa taaataatgt ctaaaagaag gttccaaata
      901 aacttctcca gtacccttgt atacaggttt catcattgat tcattagtaa gtgctcctga
      961 tattcctttt ttaaaaagac ctccaacacc accaatctta gtttcagaag atatatttcc
     1021 tttataatag tataatgcac cagcttctgt ttttattgat gaattattta attttatctt
     1081 aatttgtcta gctttcaaac cagcttctct agcaaaatat aatcccattg ccattaatgg
     1141 gctagaagct ccctcaagtt tttcaaattc taatatctca aattcgcttt tgccatctga
     1201 tgcattttct aaaattttta cttttttgct taaatccata caaatttctc cccactatga
     1261 taaaatttat ctctaaaata ttataagata taactctata ttacattttt tttgaaaaaa
     1321 tcgctacttt atcatcaaat ttttacatat ttttaagaag tttttgaaat gtttagattt
     1381 aagttaagtt atttaaggat gtatagttat ttcaatagac attttgagta ttatttatca
     1441 tttgtgggta ttatatttta tgggcatgtt aaaattagtt agaagttaga agttagaagt
     1501 tagaagttag aagttagaag ttagaagtta gaagatttat aatatgtaat atttttgttt
     1561 tgaaatttat aaattagtat tactggttag agttagttta gtattaagta tattcgaagt
     1621 agttttttag ctattttcat acatttatat tacttaatta tatatagcct aactaactta
     1681 atataccagt gaaatattga ctaaaatgcc taaatatgtt atcatacaag tcagtcaaat
     1741 aatttggcaa cattaaatat ttagtaaatt tctaacattt atggaagaaa tttctagcaa
     1801 agaaggtgag atttatatgg ctggtaaaaa aatattagca acaaacaaaa gagcaagaca
     1861 tgaatatttt atagaagaaa cttatgaatg tggaattgaa ttaaaaggaa cagaagtaaa
     1921 atcaataaga caaggcaaag ttaacctaag tgatggattt gcatctgtag ataatagcga
     1981 agtattttta aaacaagttc atataagtcc atatgagcaa ggaaatatat tcaatgttga
     2041 ttctttaaga gttagaaaac tattacttca taagcatgaa ataagaaagc ttattggagc
     2101 aactactata aaggggtatt cacttatacc acttagtttg tatctaaaaa acggtaaagt
     2161 taaagtagag attgccttag caaaaggtaa aaaactatat gataaacgtc aagatatggc
     2221 taagaaagat gcacaaagac gtatagaaag agaaatgaca ggtaagtatt aaactagata
     2281 aaactgtatt ttttagaata aaattcttta ttatctagaa cataacattg atttttattc
     2341 ttaaaagtat tatattatat atacattaaa aatttaataa aaatggtgat aaagtttgcg
     2401 aaaagtctac tggactataa actactcaag ggggcgtaaa ggtttcgacg tgggtttgga
     2461 acttgaggct gcatgtcgtg ttactctggg tcacgtaaaa actggggaac tttaaaataa
     2521 acgcagacga taatttcgcg atagcagcct agttgctgct cgtccctctt agtcctcctg
     2581 ccgactaaga ctggacgtca ttatgcaggg aactacttta ggggtgtctc gacttaaagt
     2641 agattaattg ggactggcta agcatagagc ctgccacttg gcgctatgtg aggtgagata
     2701 ttagtgaagt tggctaagca tgtagatgca gcaaggaaaa gatttgcgga caggggttcg
     2761 actcccctcg tctccaccaa ttttgaaaat gaaacaattg aatgattggg attacttagt
     2821 tttaggctaa gcaatccttt ttattttgtt ggaaagcaca caatcagcac acaaccatgg
     2881 atttacatta aaaatggtaa gatttaatat accctatttc tatcaaaaaa tatcatttat
     2941 tttatcaaca gcgtcttgct tggtattgtg cataacgtgt gtatatatat ccatagttat
     3001 acttatatca taatgaccca ttaacgtctg aacagtctta ggagggacac cagcctcgaa
     3061 taatcgtgtt gcatatgtat gtctaagagc atgaaattta attttaggta tatttaattt
     3121 ttttagcaaa gaatctaaaa ttttaccagg tctattattg tttaaagcaa gacctgtatc
     3181 attacaaatt acataattat tatttatata agcatcacct aacaataatc tttgttctgt
     3241 ttgttgtatt ttatgtcttt tcaatttaac taaaatattt cttggaatgg gaactgttct
     3301 tatagaattt ttagtctttg gatactgctc aataatcttt ttttctctag ttccatcttt
     3361 ctttatctca gtaactcgtt gtaaagtcct gttaacagtt aaagactcat catcaaaact
     3421 aatatcactc catttcaaac caagtaattc tcctagtctt aaacctgtac tcaaagcaag
     3481 gagaaaaagt atttcaaact tatctccatc aatgctatca ataaattttt tttggtcagt
     3541 aatacttaat atttgtacct cttttatctt gttatcagat ggtagtgtta ccattttaca
     3601 ataatttttt tgaatataac cttgtttttc tgcttctgtt aggcaaggct ttaatcttgt
     3661 atttagagtt ataattgttg atgcaggttt gttatataca tccataagtt tattgtaata
     3721 tctttgtata tgtgtagctc ttaaatctat taattttatt ttcccaagtt cagtatcttt
     3781 tatgtaattt ctataaatac cctcatatct ttcaaaagat ttaggtttta aatcttttgc
     3841 cctataatca aataaccaag tataaaacca atcctcaaag gttattttat cagctgagac
     3901 tatagagcct aagagcatct ttgtcctata aatatctaat ttttctttta cctctttttg
     3961 tgtttttcca gtaaattgtt ttcttataag tttgcctttt tcatcacgac caatacttat
     4021 agaagcacgc catcctatct gaacaccatt tcttatattt ttaacaatag acccctcgcc
     4081 attagctctt ttagttgcca ctttgtcacc tcttatacag tattaattat atttattata
     4141 ctacataatt atcttttttt attatacgca ttttctttgt tgataaaata atttgataat
     4201 ttataagcac taatatctaa tgcttttgat aatgatataa tttgtcgtat agtagggcta
     4261 tttataaaaa tatcttgttc taagcgtgaa atataacttt gactaatatt agcaatttta
     4321 cttaattgtt tttgagttaa gtttttattt tttctagctt gttttagcat tagatgtcac
     4381 ccaatattat tatatgtcga agtaagtcac aatattcgtc gtacgaatat ttctgctatg
     4441 gtaaaatatg gtaatatata agtataaata actcatgtac ataagattat ttaaaagtag
     4501 aaattttaat tttttaatca aaataggggt gattttatgt gcaaaaataa gataaaaaaa
     4561 gcaatttcta taaatgaatt aaaaaaaaag aataaagaag attatgatta ttttataaaa
     4621 tttgtaatta aagtcgcaga aaaaaagaat aaatgagata gattcatgtt attagcttta
     4681 ataaattatg aaaaatacat tggcacattg tcaatgtatt ttattttgtc aaacaattca
     4741 aaaggataaa ctataaatct aattattttt taggtgttaa acaacaattt tcagaccaga
     4801 tagaaacaaa tttaaaaaat gtgtatgttg ataaaaatga gttttacatt ttaaaaggta
     4861 aaacctctaa gcagggttga agacctattg atgaacacat aattaaattg gaaataaaga
     4921 ttatttcaca atccccttta aaagggagcg tcagattcta atagagtata ctttgaaatt
     4981 agaaatagca aaagaaaaat gtattttatt ttgtcgaacg attcagaact atatattgac
     5041 atatttttct tatttaaaac gaaattattg tataataaaa ataacaaact ataaatccaa
     5101 tcattttagg gggaggcgat tatgtggatt tttcagataa aataaaacag tttagtaaaa
     5161 gaatagaggt gataaaggaa aatttaacta cagaagaagc aacaaaaaca gcattaataa
     5221 tgcctttttt ccaaatatta gaatatgatg tatttaaccc attagaattt ataccagaat
     5281 atgtagcaga tgttggtgtt aaaagaggtg aaaaagtaga ctatgcgata ttggatgatg
     5341 gtaagccaat catactaata gaggctaaga atataaagga caaacttact aaacatgatg
     5401 cacaattgtt cagatatttt acagctacag aggcaaggtt cgcaatactt acaaatggaa
     5461 taatttataa gttttttaca gatttggaag aaaaaaataa aatggatgaa aaaccattta
     5521 tgaccataaa tttattagat atagatgaaa ataaaatatc tcacttaaag aaattcgcaa
     5581 aaacaacttt tgatgtagat gtagttttta acatagcatc tgaatcaaag tatactaatc
     5641 tgataaaaga gcaactaaat gaacaacttg agagtccatc tgatgaattt gtacgattta
     5701 taataaacga tttttataat ggtgtgaaaa cgcagaatgt tgttgaaaaa ttcagaccta
     5761 ttgtaaaaag gtctattcta cagtttgcga ctgattttat gaatgaaaag ttaaagtcat
     5821 tgttagaaag taatgatgat gaagacgaaa aagagaaaaa gaagataatt gataaaaaag
     5881 ctaaagagga aactgaaaga aaaaataaag agaagactga aaaagaaaaa atgagaaaag
     5941 ttgaagaatg ccaaaaagaa gaaacaaaaa tagaggttgt taaggaagac cagcaacaaa
     6001 taagtgctga ggaaaaagaa gcttttgata tagtacaaga tattttagga gatttatcat
     6061 gcaaagaaga tataacctat aagaaagaaa aagagtggtt aaatatatta tataaagctg
     6121 attccaattt atggatatgt aacataaagc ttacttcttc tgataagtta atctctttac
     6181 caaatcaaaa taatgagcct gtagagtatc gtatggatga agtcatagat ataagagaat
     6241 tgaaagatga gttaatattt gtactatcaa agtataccaa ttaagaaata taaataaaaa
     6301 atacattgac taattcagtc aatgtatttt ttatttatat ttctttgtta gagcattgag
     6361 cattttaaaa actgcttctc tatcttcttc atcaagttta aatattatat cagttatttt
     6421 ttttacatca tcatcaattt ctaaatcttc taaagtacta tcatatgttt ttttatcttc
     6481 tgtaaatcct aaaagataat caatgctaac ttcaaagtaa tttgctaagg tacaaagagt
     6541 atcaaaatca ggttgttttc tatctgtctc atagcctgct atagttgacc tttgcttatt
     6601 aataatttta gctaaatcca tttgagtcaa attatgttct tttcttaact gtgctagtcg
     6661 ttcataaaat ttactcaatt aaatcactct ccataagtaa gattatatcc aaaatggttt
     6721 ctataagtaa ctacatgttt ctaaaaaaaa ctttttctct aaaaaaactt gacaaagttg
     6781 ctaaaagaaa ctaatatata aaatataagg tttccaaaag aaacattaaa aggagggata
     6841 ttatgagaag caagttaaag caattaagaa taaaaaagaa cttaacacat aaagttatgg
     6901 ctaaagagct tggaatatca cgttcttatt atacttcaat agaaatggga aataaaacgc
     6961 cctctctaaa aacaagtatg aaaataaaat catttttgaa atattataag gatgatattt
     7021 ttttagatga taaagtttct aaaagaaaca accttgatta attctatatc aaaattggag
     7081 gaaagtaaat gttacaacaa tgccaaaata tatatcaaat tagtagggaa aatgctagat
     7141 tgacacaaga aaaagcatca gagttattgg gtatatcagt agaaagtatt agagcttatg
     7201 aaaatgataa aagaatccca aataatacag tagtagctaa aatggtttct atatataaca
     7261 ataaactatt aggatatgaa catgttagaa gaaacacaga ggcggggata atgttcttac
     7321 cagaattaaa aataaaaaac ctttcaagta tagctttaaa gttacataaa gaaataaaag
     7381 attatttaaa aaaagaagat gacttcatag atatagttga agatgatgta attgacgaag
     7441 aggaagaaga agtttggaat gatgtaatga acgaattaga tggtatatat gaagctatat
     7501 ttgcattaaa atttgctaag aaataggagg tatttttatg gaagttaatg aaaatatgca
     7561 aacaaaagtt gtaaatctta aaactaaaaa atctagaaaa ataatgactg taaaagagtt
     7621 ttctaaggaa tatgaaatag gcataaataa tgcttatgaa atgactcata taaaaggatt
     7681 tcctgcgttt acaataggta gaaaaatatg tattttaaga gataaagttg atgagtggtt
     7741 tttagacaat attggcaaca aattctaaaa tgttaagaag taggtgacaa agtgggagag
     7801 ctaggttgga taaaactaca cagagcatta aaagacaatc ctacatggaa agcttgtaaa
     7861 gctgagagaa aagttataat gataactatg cttttattag cgagtcataa agaaacggtt
     7921 ataactttaa acacagggaa aaaagttaaa atattaccag gtcaatttat tacttctagg
     7981 agtagtttag cagaaaaatg tgggaaagat gtttctgaac aaatggttag aagtgcatta
     8041 cagtttttta aaaagactaa tttttcaacc aacgaatcaa ccaatggcta tactcttgta
     8101 actattataa actgggggtt gtatcaagat gtggagtcaa agcaaccaac tagtcaacca
     8161 gtagtcaacc aggggtcaac cacaatcaag aatgtaaaga atgtaaagaa taaaaaacta
     8221 tatgtcgaga cttctaacga gtatcgacta gcagagtttt tatttaaaca tataagaaaa
     8281 aataatccta atgccaaaac acctaattta caaagttggg caaaagattt tgattatatt
     8341 cttaggttgg ataaaagaaa tttagaagag gttcaagagt taattagatt ttgtcagaac
     8401 gatagtttct ggtgtacaaa tattttaagt gcttctaagt tcagaaagca gtatgaccag
     8461 ttatatttaa aatataagaa tgaaaaaaat ccattaaata aaaaagctaa agaagatgga
     8521 gtatataatc catatgatac agacttaatg atttaggaga ggagtaagat atgagtagtg
     8581 taaatgattt agttaaaagt acagaagcag agcaatctgt tttaggaagt atactgttag
     8641 atgcaacagc aatacgagaa atagaaccaa ttttaaccga agatatgttt taccatgagg
     8701 aacataaaat tatatttgat tcaatgaaaa agatatataa aagaaatgag cctattgatt
     8761 taattacatt gacagaagag gtaaaacata aaggtcactt aaatcgtgtt ggaggtataa
     8821 gttatataac tagtttatca aatgtagtcc caagtctatc aaatgcttta tatcatgcaa
     8881 atattgtgtt agacaaatat aaaaatagaa atataataga caaatttaat aaatttaaac
     8941 ttggtgaaat agatgataat acattgatat atcaattaga gcaagacata aataagatag
     9001 ataaaaaact gattaaaaaa gatactagta tatcagctat aacaaccagg gcgttcgact
     9061 atatatctga tggagtagaa gaaggtatag aaataggaat agaagtttta gatgaaacta
     9121 taggtggact ataccaagga gaattaacta ctatagcagc taagagtgga ggaggaaaaa
     9181 cagctcttgc actacagata ctaagaaacg ttatttttca agggaaaaaa gttttattta
     9241 taagtggaga aatgagtgat atacaaattt tatttagaaa tatatcctca ctaactggaa
     9301 taccaattgt aaaaatgaag aacagagact taaatgaaaa agaaatgatt gattatataa
     9361 atgctctagg tattctaaac caagacgaaa ttttggatat aaatgatact gtaaatacca
     9421 tatctggaat taagagagaa ataaaaagat taaaaccaga tttagttata gttgattact
     9481 tacaaatttt agattatgaa ggtaaagagg taggagaggc taagtttgct gagttatcaa
     9541 gagaaatgaa aaagataacg ttagatttta acataccagt tattcaatta gcacaattga
     9601 atgatgaaat gagagacgaa agaccaaagg gtgaccgacc tatgcgtagt agtaagcaaa
     9661 tttatatgga tagtaacaat gttgtttatc tgcatagacc tacagaaaaa gaagctctag
     9721 agtattgtaa attaattggt attgaagaca tagaagcact tagaaaaagg gaaaaaagtt
     9781 taggtgtaaa gttaactgaa ataatagtag ctaaaaatag agatggagag gctagatata
     9841 ctccatattg gtatgttgga tgtagattaa cttttaaaaa atgtactatg gcaaaaataa
     9901 tataaggagg taaatatcaa tgtatgaagt agaactaatg gagttaatag acaaaagtaa
     9961 agatgggaat gaatcagctt ttgagtcatt attaaaaata tttgataaag atatacaaat
    10021 aaatgcaaat aagtatgtaa atttattaca aggcggtttt gattataacg atttaattca
    10081 ggtcgcgaga atagcactat ggaaagcgtt aagtaactat agtcacacta aaggtaaatt
    10141 gcatcctaaa gcatatttta aaaaagctat aaaattatcc atgttatcat tattaaatag
    10201 tggaaataga aaaaaacata gaactctcaa taaaagtata tctttaaaca attttattag
    10261 taaagatgaa caaattgagc ttataacact tatagacaat aatgaggatg atatagaatc
    10321 acaagttata tctaaagaga taacgagaga acttaaaaat aaagtgaaaa aagatattgt
    10381 atatcaaacc gatatgcaaa gaaaggtgat tagtttacgt gtagatggat atgaagggcg
    10441 tgaaatagaa agaatcttag gaatatctct taagagtata aattgtcatc tatacagagc
    10501 taggaaaata ataaaacaaa atctaggaag tgttaattat gaagcttaaa aagttaatat
    10561 caaaattgag cagacttgaa gatgttgaaa tagtttttca tataaatgtg aaaaacatac
    10621 ctataataag tagcataaaa ttgagtagta ttattatgaa tcatgatgaa gtagagatat
    10681 atggattgaa taattttaaa ttatatatcc cagtagatga aataatagat atagttgaat
    10741 atccagagtc attaatattg tatagtttaa gttttcaagt aatattcaga tggtaaaagg
    10801 ggtgtaaata tgactttaga tttaaatata aaagaattat atgaactagg attttcagaa
    10861 gaatttatag ctttagcaaa agctcattta ttagatgatt ttatctttaa tgatgtacca
    10921 aaactgtctt atacaaatgt aggcaaaatg tatgatataa aaaaatatga gatttttatt
    10981 gttgtgaacg aaacattaat atatatttta caaacacaat ataaagaaga gtttttgcct
    11041 gtagaagaac aaattttatc ctttggaact gtaaagaaga tgttagaata tgtttgcaaa
    11101 taaaaaagat gttttcttaa gaaaacacct taagtgtatg gaatacacta taccctcgac
    11161 aagtatagta tatcctatat tcttaaaaaa atcaattata aggggaagtt tattatgaga
    11221 gatattaaga ttagagatat aaaagagata acattgagtg aagcatatag tatttttaaa
    11281 gtcttaaaat gtggatttat cataaaagat ggaaaactaa aaggtttcac aaaataataa
    11341 ttgaatggag tggtgttatg agtgatttaa aaagtgagtc gtttaaaaag gcagagaata
    11401 aattatatag ttataacaga gtaaaaacag aaataaaaaa tttgaactta gaaatagaag
    11461 aaaaagaaaa tttatatatg ggttgttgct ctataggata tggagaaaag tctggaacta
    11521 cttataatat ttctaatttg gttgaaagtg aagttctaaa aaaagaaaaa gaagttgaaa
    11581 aaataaagag gtatataaaa gtgaaagaga tttttataga aaaaattgat aatgcaatat
    11641 tattactaaa tgaagatgaa attgcattaa ttaagcatag gtactttagt aataaaaaaa
    11701 attcatggaa ctatgtagct aataaaatag gtttttctac tgcaagatgt aaacaaatgc
    11761 gtatagagat tataaataag ttaaaagatt tattgagtta attacattgg ctagtgacaa
    11821 aattaccatt atacctatta agttgggttt tatgtagtga ttatactaaa agtgtaaaaa
    11881 aagtgtttga gttatgacca aaaattatac taaaactata cgattattat actaaatgta
    11941 tactcttttt atacttaact tgttatataa tgaagatagg gttaagtagt taactatgaa
    12001 tagaatatta gcataagtat attaagttgt agagtagaag ttgttaatca tactctaatt
    12061 aggctagatt ataaatctag tctttttttt attccttctt tgaagtttta gtacaatata
    12121 tacattcttt ataatgattg gtattattag ataaaaggag gtatgtttta tcatgttgta
    12181 tgatataact agtataaatg gagcatataa atttttaagt gaatatttag aggttgatag
    12241 agattatata gacttgtata taagaaggaa tccatcaaaa tataaattta aaaaatttat
    12301 agaagaaata gagtttataa aaaaaataaa atttaataat ttaaaactag aagacataga
    12361 cattattctt atacacatta caacaaatga tgatgattgt aattctataa aaaaatatgg
    12421 tctaattaat ttaatacagg ctataacttt agaaactcca ttaagtaaat atttaaagag
    12481 ttttaatatt gtttttgatg tatataacta tctaattttt attgatgaac aagtattttc
    12541 tataagagat tataaagaaa gtaaagaagt tataagatta ttgtatttaa agttatatga
    12601 tgattataat ataaatgctt ttttttatgt tagagatttt aaatcatatg gtggtagagt
    12661 gcatgaaaaa ccagagatat tatgtaattt aagcaaagca tttaataaca aaaatatatc
    12721 tgaaaaatgg aaaaataata tacataataa atgctattct gtaaagttca aagttagatt
    12781 agaagagtta gagtggtata ctttttatga caatattgag gaatacaaga aagattatac
    12841 taaaaaagat ttaataagaa atgaattggt agaaaatgct cttagtgtaa tacatgaaga
    12901 ttatttcaaa aaagactcaa aatcagaact aattgcatat ttaaaaagag aagtattgat
    12961 tccttatagt aatataattg aaattgaaga aataaaatag aaaagtttat ataaaattat
    13021 aatttattag atttttgata caactgaatt atggagcatg aaaattattt taatgaataa
    13081 acttatttta tgaaaggcta gatttaataa tctagtcttt ttttatactt aatattgaaa
    13141 ggagttgtta tattatatgg gaagatataa gttgtataat cgataaagaa tgtgaactat
    13201 ctaaagatgg aaagagctaa aaaaataaat ggaaattatt cctaggttta ataaatctaa
    13261 agaaatgggg ggaagaaatg taaaaggtga aaagacccgc aaagccaata aaaaagcaac
    13321 aagatgtact cgacatccaa gattatctaa gatataaaag ttatagaaat tatgtaatct
    13381 ttatacttgg tataacaaca ggatatagag caggagattt agttaaatta aaagtaagag
    13441 aagtaaaaga agctttaaaa agacaagaat ttactatttg ggaaggtaag aaagtaaata
    13501 gcaagaatat aagagaaaaa aatagaaagc ctaggacagt agaaataaga ccaaaagtca
    13561 cacatatttt aaaaaaatat ataaaaaata aaaaagatta tgagtatatg tttccatcaa
    13621 gaaaaaaaga ttatccttat attggagtag ctgcaattag taaaattctt aaagaagcag
    13681 gagaatattt tggattatat gatataacag cgcatagtat gcgtaaaact tatgcttata
    13741 aaatatatat tgatagtgga aaagatattg tggcagttaa agaattgcta gggcattctt
    13801 cgatagaaga aacgaaggca tatttaggtt tagataaaga gctatatcat cattacagta
    13861 aatcgttaga cgattttgtg agatgatatt tttttatttg cctgtttgaa tgtctaaaaa
    13921 tttggtgtat tactatttaa ggtgtcaaat tctctatata agaagtagga aaaatttaat
    13981 ttgaatgttt gatttactaa gaaaatagag gattcaaatg aaaaaatacg aactatattt
    14041 attaaaaata taataatatt cgtattgaaa agaggtgttt ttatgaaaga aatagagtta
    14101 agtaataaag agattgaact gattatttca gtattagatt ctaaaatttc tagcaactta
    14161 gataaagttt ttggtgatgt tcaatatagt cctacttgta aacatagact tagaaaagaa
    14221 aattctatca tagaaaattt aatagataaa ttaatagata ggcttaatgg aatagaagtt
    14281 gaaggaaaag aaaatgtttt aaggatagat tggaacatgg aagaatttga ggggaagctt
    14341 aaagaagtta ttgaggaata tctttgtaat ggctagagag ttcagtcaaa gcttttataa
    14401 tagtaaagca tggaaagatt gtaggcaagc aattgttaag aagtatctag gcttgtgtgc
    14461 tgagtgtggg aagttaggag aagaagttca tcacataaaa catttaactc ctagtaatat
    14521 aaatgatgtt gatatagcct taggtgagga aaatttaata ttgctatgta aggactgtca
    14581 tagtaaaaaa cataagagta agaaagatat tactagagca ggattaaaat ttaatgaaaa
    14641 tggggaattg atttcgattt agaaaaaaat agcatatccc cccctaaaaa cgaccctggg
    14701 gggctgattt caaataccga tgtccccacg tcaattttcc tccgcatgaa aaaatcagaa
    14761 tgggaggggg gttatttaaa ataatttacg aacaattaga gaaagaaaaa aagataaaac
    14821 aagaggtaag cagattaaag aaaaactata aagatttaga aaaagaaaaa gttaaaattt
    14881 tagatgggtt agtaaatgaa gcagcttttt taaaaatatc tttagaagag actagagaaa
    14941 ttttgacaaa agagggtttg actgaaattt tcaagcaagg caagcaggaa tttgaaaggg
    15001 aaagacttca agttaagata tatttaaatt ttatgaaact ttattctaat gtcatgaagc
    15061 aactaattga tattattcca agcgatatga aacaggaaga agaggacgaa cttattaagt
    15121 ttataaaaaa aggtagactt aataaatgac ctacattgaa gagtattatc aaaaaatatt
    15181 aaatggagaa atagttgctt gtagcagaat taagcaggtg tacaaaaagc ttgtccaaga
    15241 cctatataat ccaaaaggaa attgggtttt tgatgaagag cttgctaata gacctatcga
    15301 gtttatagag actttttgta agcaagcaca aggtaagttg ggtgaacctt tgaaacttga
    15361 attattccaa aaagccaagc atcaagctgt gtggggtttt gttgataaag agactaggtt
    15421 taggaaatat caagaagtac ttgatattcg gggtcgtaaa aatggcaaga ctacagaatt
    15481 ggcagcggat gaattattta tgttaattgc agataatgaa ggttcgccag aggtttataa
    15541 tattgcaact aaatacgaac aggcacaaaa agggtttaaa gagtgctaca aaatggtaca
    15601 gcaatctaaa attttatcta agcatattaa aaaaagaaaa tcagacctct attttcacgc
    15661 taattatggt tttttacagg cacttgctag caatagcaat gggcttgatg gattaaactc
    15721 gcatatggta actatagatg aattggcagc tataaaaaat agagatattt acgacctaat
    15781 gaaacaatct atgggtgcaa gaaatcaacc ccttttaaat tgcattacca caaatggttt
    15841 tgtgagagag ggtatttttg atgcacaata tgagtatgct tgcaatgttt tagatggaaa
    15901 aataaaagat gataggttta tagcttttat ttatgagtta gacgataagg atgaatggga
    15961 cagagaagaa tgttggataa aagctaatcc aggtttaggg actataaaaa agcttgattt
    16021 tttaagagat tgtgttaata aagctaaggc agacccaaac tttaagccta ctgtcatggt
    16081 gaaagatttc aatatgaaag aaaattctgc gactgcttgg ttaagatggg atgaactaaa
    16141 caatgaaact aaattcaatg cagatgaaat gggatttaga tatggaatag gttgctttga
    16201 cttagctgaa actacagacc tcgcatctgc taaagttttg ttaaggaaaa gatatgatga
    16261 taatatttac gttctttcta tgtactggat tccatttgag cgattagaac aaaaaactga
    16321 tgaagataaa attccgtaca ttttatggga aaagcaaggt ttgttaagaa tctgcgaagg
    16381 aaataaaata aatccatatg acatcttgcg atggtttaga gaaatcaaag aggagtatga
    16441 catttatatt ccatggattg ggtttgaccc ttggcatgtt gactcgagtt tgctgttggc
    16501 ttatgagaat gagtttggaa aagattctat gattaaggtt aggcaagggg cttatacttt
    16561 atctgctcct atgaaagaat taagagctga tttaaaagct aataaagtta tttataataa
    16621 taattctatt gataaatggt gccttagtaa tacagaaata aaaacggata taaatggaaa
    16681 tatacaacca ataaaaggtg ttgataggcg aaaacgtatt gatggagctg tatctttgat
    16741 aattggttat gttgttctga aagaaaaaat gtcagaatat gaaaacatga tttaggtgag
    16801 ggggtgaaaa atgaatatat ttaaatctaa gaagaaaaat aaagaagctc ctgggaaagt
    16861 tgctatagag cttattacag attcgggaaa cgggttttat agttggtgcg gcaatttata
    16921 cagaagcgac attataagaa gtattatacg accaaaaagt aaagctgttg gcaaaatgac
    16981 agctaaacat attagaagta atgagactga atttaagacc aatccggaaa tttacattaa
    17041 atttttgctt gaaaatccta atccattcat gagtggtcaa atacttcaag agaaaatggt
    17101 tactcagtta gaacttaata gtaatgcttt tgctgtaatt attaaagata gttataatat
    17161 accaattcag atttaccctc ttaacgcttt aaatgttgaa gctatttatg aagatgaagt
    17221 tttgtttttg aaattttcgc ttaagaatgg gaaaatagtt acttatccat attcgaacat
    17281 aattcatcta agaaaagatt ttaacgaaaa tgatttattt ggaacacctc caactaaagt
    17341 gcttgagccg cttatggaag ttgtaaacac aacagaccag ggagtcgtaa aagctatcaa
    17401 aaatagtaac acaatcaaat ggttattaaa atttaaaaca gcacttagac ccgacgacat
    17461 aaagaaagag gtcaaggaat ttgaaaaaaa ttatttgcaa atagactcag acgcaggcgg
    17521 agctgctgca actgattcga aatatgatgc tgaacaggtc aaagctgaga gttatgttcc
    17581 taatgctgca caaatggata aagcaataca gagattatat tcgtttttta atacaaatga
    17641 gaaaataatt caaagtaaat atacagaaga cgaatggaat gcttattatg agtctgaaat
    17701 tgaaccagtt ggattgcaat tatctaatca gtatacagaa aaacttttta ctagaaaagc
    17761 tcgaagcttt ggaaatgaga taatttttga agcttctaat ttacagtatg caagtatgtc
    17821 cactaaatta aacttggttc aaatgattga caggggttca cttactccga atgaatggcg
    17881 taaaataatg aacctttcgc caatagaggg tggaaacaag ccagttcgtc gcttggatac
    17941 tgctgttgtg gaaggaggtg agtaaaataa atggctaatg acaatttaaa tgagttttta
    18001 aaaatcaaaa actcaacaga aacaagttca agcctctatt tctatggaga tattgtttgt
    18061 gatgagtggg acgcttggac agaagaagac cagtacccac tttctattaa aaacttttta
    18121 gcgactgaac aaggaaaaga tttaaatatt tatattaatt ccggtggtgg gtctgtattt
    18181 gcgggcatgg caatatataa tatactaaaa agacatgaag ggtttaaaac tgtttatgtg
    18241 gatggtattg ctgcaagtat tgcgagtgtt atagcattag caggtgataa agttgtaatc
    18301 cccgaaaatg cttattttat gattcataaa ccttggttgg gtttatttgg tgcttataat
    18361 tcagacaaat taattaaagc atcacaagat ttagacagga tagaagaagg cattttaaat
    18421 gtgtataaag ataatttgag agaaggaatt gatattgaag aaataaaaga aaagctaaga
    18481 gaagaaactt ggttcactgg taaagaagca tcaaattatt ttaattttga ggttgatgaa
    18541 aaaaaagaag ttgctgcttg tgtgagtgat tattttgata agtacaataa aatgcctaac
    18601 attttaaaaa gtaaaataga caaagatagt aataaaaaag aaaataataa taaaaaaaga
    18661 gttcagctaa ggctggactt attaaaatta gggggtttaa atgactagag aagaatattt
    18721 taagaaaaga caagaaatga tagacgaagc acaaaaatta cttgatgatg aagttgggga
    18781 ggaaggagca ggagaggaaa atactgaaga agctgaaaaa atagctaata ggataaaagc
    18841 tttagacgag gaatatgaaa gaaatgtaaa agctagggcg aatttaaaag cattgcagga
    18901 taatgtcaaa atcgacccta ctatttttaa tttaactaat aacaaaggta aaatagaagg
    18961 tatagaagac acaactgtta aggataaaca agaacaatat aaaaatgctt gggctaaaga
    19021 tatgttagga aagcagttaa attcagaaga acaggagata tttaatagta ttaactcagc
    19081 atatagagcg gaagttcaaa caagcgaaaa taatactatt ttaattccta aaactgttgc
    19141 ttcgggtata tggaaagaaa ttggtgacat gtatccgtta ttcggagatg cttctccaac
    19201 ttttgtagca ggtgatttga cgattatagc agaagaagat ggtggtgatg atgctgcgtg
    19261 gtatgatgaa gaaacagaag ttaaagagga tggttataag ctaaaggaaa taaccttaag
    19321 aggttgtgag cttgcaaaag atataactgt gtcgtggaaa ttgaaaaaaa tgagtattga
    19381 tgaatttgtt ccatatataa ctagtctttt agctgaaaaa atgggtgcag cattggcgaa
    19441 agcgattgta gatggaaaag gaaaaccagg ggaaagtgat tcttttaaac ctcagccgct
    19501 tggaataaaa actgctttaa gcaaggaagc tagtaaggca caaataatag aatatactga
    19561 taagatagct tatacagata taacgaaatt gatgtctgtt ttaaagaaat ggagcaatgg
    19621 agcttgtatt tatgctaata gcacaactat atggacacaa ttagctgaaa tattagatac
    19681 aactggaaag cctattttta tccctgacgc tgcaaatagt gacggagttg gaaggatgtt
    19741 tggcagggtt gttaaaatgg atgacagcat ggcagatggt gagattcttg ctggaaatat
    19801 agcgaaaggt tatgcgataa atataaatga gaatgtaact ttatatacag atgaacatgt
    19861 aaaaagtaga aaaacagatt atttaactta ttcattagtt gatggcaatg ttataagtaa
    19921 taaagctttt ggtatgatag ttaaaaaaac tagtgcagtt gcgaagtagg tgttttgaat
    19981 gattgtatca ttagaagaaa taaaagaata tttgagatta gaggcagatt ataaagaaga
    20041 tgataatctg cttttgtctt ttttgaaagc agcggaagaa gatttggaaa atcgaacagg
    20101 aaaagttttt aatgaaaata acaaatctga gcttgttggc ttatatataa aaatgtattc
    20161 tgcagagcag tacgaaaaaa gaggggcaac agaaagtaat agcgaaaaag ttagatttgt
    20221 tttagaaagt ataatatctc aaatttctat atgtagtagg tactaaaatg gacgttggaa
    20281 aattaactca gagaataaaa atacaaattt atggagaaat tgaaaacgac ataggagaaa
    20341 ttacaaaagg atggtctacg tataaaaaac tttgggctaa caaatcgttg cttagaaata
    20401 gtaataatta tgtgttagat aaagaaaata tagagtattc ttacagattt aaaataagat
    20461 ataggactga tataacagaa gctatgagaa tagtttgtaa tgatgttgtc tatgatataa
    20521 agcatgtaaa taatataaaa gagctaaata aatatgaaac aaatattgat tgtattcttt
    20581 ataaagaagg tgtttacaat gagtaataca gattttaata tgaatggatt agatgattat
    20641 acaaataaac tatttaagcg tattgtaaaa gaatacccca aaaaagctga gaaacttatg
    20701 aatactagct tagggaagtg caaaggagaa gctattgcta ggactcctaa atcaagtaaa
    20761 aagcctaaga agtataaaag ggctaagcac atgaaagata attggaaaac taaagtgcag
    20821 tctaaaaatg ggaattgcac aggtgtttta aaaaatgatt ctccacacgc acatttaata
    20881 gaaaatggtt gggcaacaaa aaatggaggc tatgttgaag ggaaacatat gttgcaacag
    20941 acgatggagc atcagagagt gaaaattgat aaaaggatag agaaaatggt tgatgaaacc
    21001 tttaatcttt agagggggta agagtgttaa aaattgtttc tgtaaaaaaa gctatagttg
    21061 aaaagcttaa gtctttaaat ataaaaatag ttgcaaatga aataagaagc gggtttgaaa
    21121 agcctgcttt ttttattcaa attattcctg ttgagatgtc tagtgacccg aatttttcga
    21181 acagcacatt acttattaat atacattatt tttctaaaga aaagacagag ttagaaaatt
    21241 taaaaatgat tgataagctt aatgtattgt ttcaagattg tattttagag attgacgggg
    21301 gcgaattgac tatagaagaa aaaagtgtag aaatatatga gaatgtttta caatacaaat
    21361 ttaatttgca agtagttgaa attatagaag aagacgaaag caaatatgag cttatgaaag
    21421 agcttgaaat gaatatttag aaggaggttt tagtttggga ttaccaagtg caataattga
    21481 gtttcaaagg cgttcaaaga ctgttaaatt tagaagtcga agaggtgttg tagctttaat
    21541 acttaaagat tcaacagcta taaagaaatc ttattctatc aattttttaa cagatataaa
    21601 cgaaactgaa tttacaaaag aaaattatga ttatataaag cttgcatttt taggaaaacc
    21661 tagcaaggtt attattgaag ttattaatga ttcagttgat tctgaaaggt ctttagacga
    21721 tgctttgaag gctttaaggg agaataaatt taactattta gctattcctt ttataagtga
    21781 agaagttgac aaaactaaaa tagttaattg gataaaaata gctagaaggg aaaaagagat
    21841 atacaaagct gtgttgccaa gcgttaataa tgctaacgag aaagcaatta taaacttcac
    21901 aacagcaggg ataaaagttg gggaaaagtc ttatacaaca gcagaatata cagctagact
    21961 ggcaggtatt ttggcaggta tatcactttc agagagctgc acgtattttg ctttagatga
    22021 agttacagag atagaacctg ctgaaaatcc tgatgaagct gtagaaaaag gtaaattgat
    22081 tttaataaat aataatggaa taaggatagc taggggtgta aattcattaa taactttaag
    22141 taaagaggat acagaagact tgaagaaaat aaaaatagtt gaagctattg acatgataca
    22201 agatgatatt ctgcaaacct ggaacgagaa ttatgttggg aaagtaacta ataaatatga
    22261 taataaaata ttgtttttat ctgctgtaaa caattatttt aaagagctgc aacgtgatga
    22321 agtacttgat aatagccaag aagcttatgt acagatagat atagaagcac ataaaaaata
    22381 tctaaaagaa aaaggaattg attatagtga aatgactgaa cagcaaataa aagaagctaa
    22441 tacgggttct tacgtttttg tagaaggaaa tattaccatc actgacgcta tggaggattt
    22501 gaaatttaaa atatatatgt aagaaggtga gtagatgagc aaagaaaata tcgtaggaag
    22561 tagtcaaatt tctggtacat ggggaaagct ttggtgggat ggaactttaa ttgctgaggt
    22621 gcttagtttt gaagctaaag ttacagcaaa tagagaagag attcagtttg gaatgagtaa
    22681 agattctaaa ataacatcga taagtggcga agggactgta aaacttggaa aagtgtattc
    22741 cagggggaag aaaaaattat tggaagcttg gaaaaaagga gaagacccac gaagtacact
    22801 tacaagtaag ataaaagacc ctggtacacc tggaaaacaa gctgaaacag ttactattaa
    22861 caatgtttgg ttcaatgaat tagctttggc gcaatttgaa aaaggtggga aaatcgagga
    22921 agagctgagt ttcggattta ctccgaatga ttctgatatg atggacgaaa tagaagaaat
    22981 ttaaggatag tttttactat ccttttttat ataaatatag gaggatttta aaatggaaaa
    23041 taaaaaagaa atgataacaa tagaggatat tttaagaaga aaagaatatt ttgcaaaaaa
    23101 aagcgaagaa actaagcaat tatacattcc ttcacttggc ggaaatatag agattgcaaa
    23161 accggataga gagctatgca ttgatgttac agaaatggaa aactcaatcg aaagtgataa
    23221 gtattttgtt tatgagatag ttaaaaagcc aaatttaaag agtgaaaaat tgcatacaga
    23281 gtttgggtgc aaagataatc ctcttgacat agtcgatgta ttgtttgaga caggagaaat
    23341 tgctgatatt gcaaagattg ctgcatcatt cgcgggattt ggtgttgtgg aagaggttga
    23401 agacttaaaa aactaattaa aagcgatgtt gaaatgcaaa tgattagcca ttacttagaa
    23461 aaaggtgttg atttggacaa attgactaat ttaaatataa tagaaagaaa ttttcgcatc
    23521 gcttgtatgc tatatgaaga agaagaaaaa ataaaactta tttctgagct gataggagct
    23581 gtgtttgggg gtgtaaaaaa tggctagaag gcatataagt gcagttatat ctctaaagga
    23641 caacatgagt gcgactatga gagggattag aagagagcaa aaacaatttc aaaatgaagt
    23701 taggcggaca cgtaacgaga tgcgttcggc gagtagagag cgtatgcgta taagaatgga
    23761 tgcgacacca gcacaccgaa ctatacagaa tttaagacaa aaacttgcac ctcttcgaac
    23821 taagcttgta aaagctgttg ttataaaaga tttagcgact gaaaagatag aaagaataaa
    23881 atcgaacgta aaatcttttg gaaaatttat cgcaagacca gctataaaat taaaagatga
    23941 aacaaaagga atgattgata aaataaaaaa tcgacttact agcttatcaa ctattgttcc
    24001 tgttggggct gcgatagggg ctgcgggtat ggctgttaaa agcggtatgg aactagagca
    24061 acaacaaata agtatgcgtc acttcatggg agttggaaat aaaggaaaat ctagcaaaga
    24121 acttgacgga atgagcgcaa gctatttaaa agatttaaga aataatgcaa atgcgacgcc
    24181 atttgaaact ggagaagtta tatcagcagg aactcgttcc ttgcaaatag cgggtggaaa
    24241 tacaaaagat gctatgcaga tggtaaaatt agctgaagat atggcagcac taaatccagg
    24301 caagagcgtc ggggatgcta tggaagcact tgcggacatg aacattggag aaatggcaag
    24361 acttacggag tttggagtta aggcaagcag tacagacgac ccaaaagaag tacaaaagaa
    24421 gcttgaaaca atgtataatg gtggagctaa taagcttgca gaaagtggtt cgggactact
    24481 ttctacgata atgggtaaat taaaatctaa tattgcagat attggacttg gtatgttgga
    24541 acctttaaaa cctgttatga ctggcttaat tggctttata gaccaagcta gccctaaaat
    24601 attagaagta ggtacaaaaa taacagccgg aataggtatg gcgattgggt ggatacagca
    24661 acaaatgcca accttggctc cgatttttca aacagctttt ggagctgtat cttcgattgt
    24721 atcaactgtt gccccaataa tcggtcaagt cataggagct ttaagcccta tttttatggg
    24781 actgctttct gtggcttcgt ctgtattagc agggattgct tctgctgtta aaactgtagc
    24841 tcctgttgta agttctttaa tttctaaatt agcaccaatt ttcaaaaatg ttggtgatac
    24901 cttaaaatct atgggtaaaa tttttaaaaa tgtttttgat agtgttatga aaatagttaa
    24961 aaaagcgtct gattatataa aacctttagt tgatggaata acaggagcag caaaaggtat
    25021 aagcgatggc gtcagttggg ttgctggaaa actagctgga aatgcaactg gaacgaaata
    25081 ttggtcgggc ggactttctg ttgtaggcga acatggacca gaacttgtat ctatgccacg
    25141 tggtagcaaa gtttttacaa atgcagaaag taaatctatg attaataaaa gtattcctaa
    25201 ttttagacaa gtgcaaggag aaaacacaaa ttataatata acaattccta aaattgctga
    25261 aacaatagtg ataagagaag atgctgacat tgaaagaata acatcaagtt taataaaaaa
    25321 aatacaaatg gcgaaaatgg gtggtgtcgt ttaatggaaa tgtggcttag acaatcgaat
    25381 gatgccttta gattcccgat atttccagcc tcttttgaaa taagcggaaa tataaataca
    25441 agtacaacaa atgtactaag gctcggagaa gtaattatct gtggtggtac aggacttaga
    25501 acaacagaga taagcagctt tttcccaagt agacaatatc atttttgcaa ttataaagat
    25561 tttccccaac catatgattg tgtaaataaa ttaaaaaggt ggatggagca ggggttaatt
    25621 ttaaggtata taataactga aactgatgtg aatatggagg ttattattga aagcttcaaa
    25681 catggcaagc aagatggcac aaacgatgtt tactttacac taagcttaaa agagtataaa
    25741 agaatacaga tacctagcat aaattcctca gatgggaaaa tagaagtggt aaaaaatgta
    25801 ccagttacaa aaggttttga aactggaaaa caaaaaacac ataaagtcgt taaaggtgat
    25861 aacctttgga gcttgtcgca aaaatattat ggaaatgggg atttatacga aaaaattgtt
    25921 gaagcaaata aggaattaat taaaacagct gacataataa aagagggttg ggtgttaata
    25981 attccttagc ttggaggtga tttataatta acaatataaa gttaaaggta cacataaaaa
    26041 gtggtaatat ctatgatata actgatatag ttgaaaaagt aacttggagt ggtgattata
    26101 agtcaccatc aaggacatta gaattttcta tagtacagtc agcttctgat attaactttc
    26161 agcaaattaa tataccaata gctagtacag tctgtttcta tgtagatgag aaagaactct
    26221 ttagaggaat gataattaat aggtctaaag attcaagtaa taatagtatt agttttgttt
    26281 ctaaagatat gggattctta ttaactcaaa gcgaagtatc atacaatttt aaagataaat
    26341 tagttgaaga tattgcaaaa caagtattta atgacaataa acttgcggtt ggaaacatac
    26401 ctaaaactaa cgttaaatat acaaaaatgt ttattggtgt aactggctat gatactataa
    26461 tgagtgcata tacagaagct agtaagacaa cgaagaaaaa atatatgata gagtctaatt
    26521 tagataaatt taatgttatt gaaaaaggga ctattacact aaatgttatg tttgaagaag
    26581 ggtctaatct tattaatacg agcttttcag agagtatgga gaatgtaaaa aacaaggtat
    26641 tagtagtaga ccagtatggg aataaaataa gtgaaaaggt agacgataaa attttcaaag
    26701 atgttggagt aataatgcaa aaagttatac aacaacaaga aaatagtact gtagatatag
    26761 aaagtgagtt taaaggaata gagcagactt gcagtttaaa agggtatggt gatgtgactt
    26821 gtataactgg cagaggtgta aaggttaagg accgttatac aggtcttgta ggtctatttt
    26881 atatagacac agataaacat aattgggaca gcaacggaaa ttatgagata gacttggatt
    26941 taaattttca aaacataatg gatgaaaaaa cagccgggca ggacgaacag aaggaaagtt
    27001 ctggtttgaa tggagaaggg acattaaatg gaaaagaagt aaaagcagaa tttacagcgt
    27061 attatccttc aaacaatgct atggagggtg gatactatca agctatggat ggtaaaagac
    27121 ttgtaccttc aaacaatact tgtgctgcac ctagtaaact taaatttaaa acaaaaattc
    27181 aagcaaaatg tcctggaact aaaattgatg gtaaaactta tacagtaaca gatagaggcg
    27241 gagcgattga cttaaaaaat ggagtgtata gaatagacat attaatgtct agtgaaaaag
    27301 aatgtaatga ttttggaaga agaaaaggaa caataataat tggagatggt acaggatata
    27361 caaatgcgac aggaaaagca aaagaattaa ttagcatagc aaaaagtaaa ttaggttgta
    27421 agtatgtttg gggggcaact ggggagaata cattcgattg cagcgggttt actcagtggt
    27481 gctacaaaaa gatagggata agtattcctc gtactgcttc cgcacaaagc aaagcaggta
    27541 aaccagtaga tttgaatgat agaagcaagt ggaaagcagg agatttattg tgtagggtca
    27601 gcggaggaag taacaaccat gttgtgatgt acattggaaa taatcaaata atccattctc
    27661 cacaaacagg tgatgtggtg aaaatacagt ctgttgactc atatagaaaa gggaaagcat
    27721 atacacatgt cagaagatat ttataaaagg atggtgataa agtggctaat ccaatcaatg
    27781 aatttatagg gataataaga gaagaaggaa aatatcacaa tcaaccttct ttttttattg
    27841 gaaaaattaa aagtaaatta ccagatttaa aaatagaggt aaataacatt atattagaaa
    27901 aaaatgatat tttaatagat agttggatgc ttgacagaca gatagaaata ttcaacacag
    27961 aaacaaatca agagcataaa catgaagtta aaaatccatt tattgataaa tttgaacttg
    28021 atgacacagt aataatgttt aaaataagtg ataaatttgc tgttgtaagt aagttggtga
    28081 gcttataatg agtacaatat ttccttttat aggtgtccca gaggattata tcttacctaa
    28141 aacagaagaa ttgccaatct ttcgtgaagt ggcttggaat tttgaaaagg ataaacctat
    28201 tttagaaaat ggagatttta agattgttga aggcaatgaa gctataaagg tttgggtgta
    28261 taaatgtata aaaactaata agtatgagca tgagatttat tcatggggtt atggaacaga
    28321 actatctgaa ttgataggtc aaaaatacag caaaggactt acagaaagtg aagctagtag
    28381 gtatataaaa gaggctttat tagttaatcc atatgtttta gatgtaaata ttagtaatac
    28441 aagatttata gatgatttgc taagcgtaga tatagtcata aacacgattt atggggaggt
    28501 ggaagttaat gtatagtagt caaacctatg atgttgttaa aaataggacc ttatctaata
    28561 tagatttaga tatctataag ggtgagggtt cttttttaag tgatatggta tctcctgtta
    28621 attcagagct tgcaaaattc tatatagaac tttcatatct tcataagaaa gcttttatag
    28681 aagataactt cgacgatttt ttagataaga gagttaacga atttggagta tatagaaagc
    28741 taggaacaga agctacagga gaagtaatat tcgaggggaa ggttggaaca actattcaaa
    28801 atggaactat tatatcttac aatgagctat tattcgtagt aattaaagat atagtaatta
    28861 gttcagaaat tgaacaaaat acaagccccg tacaggcttt ggaaattgga attagatata
    28921 atatacctgc aagtacagaa tttaagctac aagacgaaat aaatggtata acaaaaattt
    28981 acaatgattt ggcttttcga ggtggtacag aaatagaaac agacaaagaa ttaaaagaaa
    29041 gattctataa gatacagaaa aatcaagcta caagtggaaa taaggcacat tacgaagcat
    29101 gggctttaga agttgaagga gtatataacg ctaaaattta tccaagatgg gatggtccag
    29161 gaactgtaaa agttttaatc tttggagaaa ataatcaagc tgttgactcg gaggtaattg
    29221 aaagatgtag agagcatatt gaggaagaaa tgccaatagg tcctatgtta accgttttaa
    29281 ctccaagcgt tttagatata agtataagtg catctataaa attagaaacg ggatatacat
    29341 tagattttgt aaaagaaagc ttcttagaga gtattaatag ctatttaata aatgttaata
    29401 aagaaataat ttacactaaa gtaagtgcaa tacttgcatc tattgagggc atacacgact
    29461 ttagtaattt actattaaat aataaagctg aaaatatagt atttgaagag gacaaagtac
    29521 caagtgttac aaatttagaa tttagtgagg tggtagttca atgaaattaa ttgataagct
    29581 accttctttt tataacaatg atattactag aaaaatacaa gaagcttatg acatagaact
    29641 agaaacactt agagaaacat atgacgatac atttgaccaa ttttttgttg atacagcaac
    29701 atggggactt gattattggg aaaatatttt atctattaaa aatagatttg atttaagtat
    29761 agaagataga agaagtaata taaaagcaaa aatgagaggt aagggtacaa caacaataga
    29821 ggttataaaa gcgataggag aagcttacac aaagactaac gttgatgtag aagtatttag
    29881 taacttattt agttttacac taagttttat aacaaataat tgtagttata acactatttt
    29941 agaattagat aagaaaatag aagaaataaa accatgtcat ttagagcata aatttgaaat
    30001 gattttattt aatcagaatg atatgtttgc aggttctaca acgaatacag gtgaaacagt
    30061 tactatatat ccttacagtc caactgatat aacttctttt ggagaattga ctataactac
    30121 aggcaatgac agaagtatgg aaaaagtaac attatatcct agacaggagg tggtataggt
    30181 tggcagaaca aaaatattat acacttttga caacgatagg aaaggcatct attgcaaatg
    30241 caacagcttt aggcaataaa gtagatttag ttaaattgca gcttggtgat ggagcaggga
    30301 cagaatataa tccaacagaa gaacaaacgt ctctaaaaaa tgtagtttgg gaaggttctg
    30361 taaataatgt aaagatagac gaagaaaatc caaactggat agtaatagaa actgtaatcc
    30421 caggtagtgt gggtggattt atgataagag aagtaggaat atttgattca aaagaacaac
    30481 taattgcagt atctaaatat ccagaaacat acaaaccaac tgctgactcg gggagtgtaa
    30541 aagacttggt tataaaaatt attttagtag tttccaatac ttcaagtgta aatttgaaag
    30601 tagacccaac agtaatttta gcaactttaa aagatataca agaattagac actaaaattg
    30661 atacaactaa gacagaatta acaagcaaca tagaaactgc taaaacagag ttaaacacta
    30721 gaattgacac agaaaatgag aaacaaaata ttaaaattga ccaacttgtt gcaggtggtg
    30781 taaatgtatc tcatacgcat attatagagg tggctgattg gattttaaat aatgaaacta
    30841 atatgtatga agtaactata aatcatccac tattgactaa aagaatacta atagctttat
    30901 atgatgaaat tggtgaagca cttacaccaa acgctagggc tattgatgat aatagtattc
    30961 ttgttagaaa tgaagaaaat attaaaatgt atgtatattt gataaatgga aatgcagaaa
    31021 ctcattttat aaatgcgact gtagatgata acagagtatc tgaaatgact acttattcgt
    31081 ctaagaaaat cgaagataga ttggttaata tagaagaaaa attgagtgga aatttatctg
    31141 atattgcaac aagtgtaaat gagttgataa cttattgtta gagaggagag tgagaaagtg
    31201 gctacagaat ggaattttga ttttaaggca gaagcacaac caattactct aaaagcaggt
    31261 aaatataaat tagagtgttg gggagctcat ggaaaggttt ggggtggaga ttctcaatct
    31321 agtggaggat actcttatgg ggaacttaca ttaaaaaaag aaactacatt atatgtatat
    31381 acaggtgcta ctggttctag taataaatat gaaaaattta catttaatgg aggaggactt
    31441 ggtgttaata atggaggagg tggagcaact gatataagac ttgtcaatgg agattggaat
    31501 aatgaacaag gtcttttgtc taggataatt gttgctggtg gtggtggagg cgcatttagt
    31561 aaaactcctg ctggcaaagg tggtggtttt aaaggcggaa atagtactaa tgatgataac
    31621 agttcgatgc ttattgtacc tggtggtaca caatatgatg gaggtagagg ttatt
//
LOCUS       DAEPXF010000024        31503 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain HB27
            SAMN13376389-rid12479423.denovo.025, whole genome shotgun sequence.
ACCESSION   DAEPXF010000024 DAEPXF010000000
VERSION     DAEPXF010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13376389
            Sequence Read Archive: SRR10518432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 31503)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31503)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001069624.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001069624.1
            Genome Coverage       :: 315x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/15/2021 01:54:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,165
            CDSs (total)                      :: 4,076
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 89
            rRNAs                             :: 10, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 10 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 19 of 69
            Pseudo Genes (incomplete)         :: 44 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 10 of 69
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31503
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13376389-rid12479423.denovo.025"
                     /strain="HB27"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="China: Hebei"
                     /collection_date="2017"
     gene            114..497
                     /locus_tag="KRL67_001022"
     CDS             114..497
                     /locus_tag="KRL67_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH1521922.1"
                     /translation="MSTDKVVGIIDEETAELAGIEYTGKIYASSGVIKHIKKKHRCQL
                     SKDIFNDIIDTIKMVLKSPEYIGSHPKKPGKSVEFIKKIDDYILVATELDTKKGYLYV
                     SSMYAIKEAKLESRVNSGRVKQYDG"
     gene            1002..1385
                     /locus_tag="KRL67_001023"
     CDS             1002..1385
                     /locus_tag="KRL67_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HBH1521923.1"
                     /translation="MNLSKKIEDKRKAKQRAEKVKKAKIATAGVVLGAVTGAVSGVLL
                     APKSGKETREDIKDASQQIAEKINMKTVDVKGKVSEKLEDKKGNFIESKKKIKKYIDN
                     KKSVENSSEEDKIIDIVEPVSVTEE"
     gene            1387..1737
                     /locus_tag="KRL67_001024"
     CDS             1387..1737
                     /locus_tag="KRL67_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH1521924.1"
                     /translation="MDNTITINISIIYVLLAILACIALVYLIIVLVKLHKILNNVGTM
                     LNENTQNINTTLNNLPNVVETFGEVGENMKDVTEVVTEVTADFLVTKDSVKSNMDVIV
                     DILTIIKNIFLDKK"
     gene            complement(1831..2289)
                     /locus_tag="KRL67_001025"
     CDS             complement(1831..2289)
                     /locus_tag="KRL67_001025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH1521925.1"
                     /translation="MIRTKVVELIATVCRENKPHKWVDENYTPYDKSGKVELMSIEDL
                     NELISSSGKADFLYSSKLQKLLKETYINQSRASYMSGCGLFWSSYWDVLEEKFEEWLY
                     NSYIFFDAEDEYLEGMEDFELECKDVLMDVIETTSIDIYVQMIKRNITNY"
     gene            complement(2334..3122)
                     /locus_tag="KRL67_001026"
     CDS             complement(2334..3122)
                     /locus_tag="KRL67_001026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HBH1521926.1"
                     /translation="MKVVIIPGHTLIGKGTGAVGYINESKETRILNDLIVKWLKIGGA
                     TVYTGRVDESSNHLADQCAIANKQETDLAIQIHFNSNVTTSTPVGTETIYKTNNGKTY
                     AERVNTRLATVFKNRGAKSDVRGLYWLNHTIAPAILIEVCFVDSKADTDYYVNNKDKV
                     AKLIAEGILNKSISNSQGGGENKVYENVIVYTGDADKVAAQILHWQLKDSLIIEASSY
                     KQGLGKKVYVVGGEANKLVKGDVVINGADRYETVKLALQEIDKL"
     gene            complement(3261..3698)
                     /locus_tag="KRL67_001027"
     CDS             complement(3261..3698)
                     /locus_tag="KRL67_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dCMP deaminase"
                     /protein_id="HBH1521927.1"
                     /translation="MNKKLENRCSWQEYFMRLCETVAERGTCDRAYVGAIIVNSENRI
                     VSTGYNGSISGDKHCSEVGHEMRDGHCIRTIHAEQNALYYCAKEGISVKDCSIYVTHF
                     PCLNCTKAIIQAGIKHIYYRTGYRIDEYAIKLLQSSNVLYTKL"
     gene            4536..4664
                     /locus_tag="KRL67_001028"
                     /pseudo
     CDS             4536..4664
                     /locus_tag="KRL67_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003378761.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
     gene            5112..5621
                     /locus_tag="KRL67_001029"
     CDS             5112..5621
                     /locus_tag="KRL67_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBH1521928.1"
                     /translation="MLSENIKTIRKSKGLSQQELAIKLNVVRQTISKWEQGLSVPDSD
                     MLISISEVLETPVSTLLGETIVESEVDNLKVISEKLEIINLQLAQRKNMRRKMFHWLF
                     ILLCAVIVIGFLVLGGLNSPYLDWNYTDPETAVFGVAFHAFEWLFVRLAPIVLIGAII
                     GSFLTRKKI"
     gene            6010..6546
                     /locus_tag="KRL67_001030"
     CDS             6010..6546
                     /locus_tag="KRL67_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HBH1521929.1"
                     /translation="MQGGVNIRQVKSFVFPVISLIFLDQISKVLIGLFLIDFEIDIIG
                     KFLRFNPVQNTNLSYGGNFIGILSNLWVLVLFNILVILVIISGYAFYKSKNEQTSYSV
                     KVIMSCGLAGTICSLIDKLFWGGSLDFLQIPSFFIFDLKDCYLTVAEIIFVIIGILHS
                     REISMKEYIYFCYRQFKR"
     gene            complement(6983..7783)
                     /locus_tag="KRL67_001031"
     CDS             complement(6983..7783)
                     /locus_tag="KRL67_001031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBH1521930.1"
                     /translation="MRLNKRSEGYINYVKGVKREKRNVLFYQLLILIGFIVIWELLAD
                     LNVINTFLFSKPSDIYNLFIQYAFSGQLFKHIGISVYETALGLVIGTVLGILVAIALW
                     WSEKLSKILDPFLVVLNALPKTALAPIIIVWVGAGIEGIVVTAVTISVVVTILSAYNY
                     FMNIDEEKIKMLKSFGASKSQILFKLILPANTGNLINLTKINIGMAWVGVIVGEFLVS
                     RYGIGYLIVYGSQVFKLDLVMMGVFVLAICAWAMYAVVNIIEKIYNSR"
     gene            complement(7978..9576)
                     /locus_tag="KRL67_001032"
     CDS             complement(7978..9576)
                     /locus_tag="KRL67_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HBH1521931.1"
                     /translation="MKAAIYSRKSRFTGKGDSIENQIQLCIDYAKSIGVKDFLIYEDE
                     GFSGSNIDRPQFKKMMQDAKDKKFSYLVCYRLDRISRNVSDFSTLIEKLNELNISFIS
                     IKEQFDTSTPMGRAMMFISSVFAQLERETIAERIKDNMYELARSGRWLGGNTPHGFNS
                     EKITFLDENLKERSMYKLQVNNEQMEVVKLIYNKYLELRSLSKLYKYIYNNGIRGPRG
                     GKFDPSSLSRILRNPAYVKANDEILDYLKNSGMDVVGTPDRKCGILTYAKNTANSIAA
                     IAKHHGVIEPEIWLEVQNQLDKNRDKTPRISTGKTALLSGLIKCGKCGSNMRITYKGK
                     KTDKDLKYYYICGTKKTLGSDSCNCKNLNGPLVESLVIDKIKNCKEESIISAFNSNNI
                     KADNVFNAFKSETNLLKNQILDKERLIKNLVIELAKNTGSTASDYIVSQIESLNNEIS
                     ILKDKINTLESSNINISSDSINIDIIVSNLKRFNDEVDNASLDDKRLLLSTIINSIVW
                     DSSLGNLNIVYMGAEMDNSVSLSN"
     gene            9905..10081
                     /locus_tag="KRL67_001033"
     CDS             9905..10081
                     /locus_tag="KRL67_001033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH1521932.1"
                     /translation="MKINVIEIFPKDKAKLNKIEMDKASWFVNIISKKYPKEALNEAF
                     STLEKELNISKANT"
     gene            10255..11385
                     /locus_tag="KRL67_001034"
     CDS             10255..11385
                     /locus_tag="KRL67_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003462216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HBH1521933.1"
                     /translation="MNNFEIKTRIKFGEGSLKALQELKNKNVLIITDPFMVKSKTIDK
                     ITSNLINSKYQVFEEIVPDPPIEVVVAGIEAFKSINPDVIIALGGGSAIDAAKSIMDF
                     SKKILKINEVEFIAIPTTSGTGSEVTSFAVITDSQKKCKYPLVSDDLLPDMAILDPEL
                     VKTVPNFITADTGMDVLTHAIEAYVSTNSNDFSDALAEKAIKLVFKYLIKAYKDGNDI
                     EAREKMHNASCLAGMAFNQASLGINHSIAHVLGGKFHIPHGRTNSILLPHVIEYNSSI
                     VGYTNTTYSDTAKKYAQIAELVGFGNGNIRISVRNLVNEIKKLQKEMNMPTKLTQCKL
                     SLEDITNEEREIAKLAIKDTCTLTNPKVPTEDDIINILNNIK"
     gene            11626..11976
                     /locus_tag="KRL67_001035"
     CDS             11626..11976
                     /locus_tag="KRL67_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003453843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMC domain-containing protein"
                     /protein_id="HBH1521934.1"
                     /translation="MTEESKQRVIQEYVPGKQVTLAHIIANPNEDIYKKLGLVLDKKD
                     AIGILTITPSEASIIAADVATKASNVSLGFIDRFSGSVVISGDVSSVESALNDVLEVL
                     GNMLNFSSTKITRT"
     gene            11984..12433
                     /locus_tag="KRL67_001036"
     CDS             11984..12433
                     /locus_tag="KRL67_001036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EutP/PduV family microcompartment system
                     protein"
                     /protein_id="HBH1521935.1"
                     /translation="MKNIIFMGKTGSGKTTLCQKLDQLELKYKKTQSVELYNNAIDTP
                     GEYMENRNLYNALITTSADAKIIAIVYDATQEENYIAPGFASMFCKDVIGIITKINKA
                     TENEVNIAYERLSLAGASQIFKIDTVDNIGLEKLFKYLEKSKNIDEV"
     gene            12535..13110
                     /locus_tag="KRL67_001037"
     CDS             12535..13110
                     /locus_tag="KRL67_001037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HBH1521936.1"
                     /translation="MGKKILLVDDEPLLRMDTKDILESHGYEIIGEASDGFEAVEMCK
                     KHKPDLVIMDIEMPILDGIKAGKIICKENLAGGVLLLTSFEDKEYVEKAKSIGAYGYL
                     VKTASEKSLIPTIEMCLNKVNEFEKMKKDLEKVNSKLNERKLVERAKGILIREFEISE
                     DEAYTRIRKLSMDKRTTMMEIAKMIIIGYDD"
     gene            13103..14512
                     /locus_tag="KRL67_001038"
     CDS             13103..14512
                     /locus_tag="KRL67_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase N-terminal domain-containing
                     protein"
                     /protein_id="HBH1521937.1"
                     /translation="MTNTIKKISTAQTYLNNNEIEKIIEVSKSLDLMASFYEADVFID
                     VLSKFDDEAIVVAHGRPKEKSMYCENVIGKKALIENEPGVIRCLKTGERVRDIKALTQ
                     EYKLVKQNIQPITLDDKVLAVLIVEKDISSEVKNEFNASEDEENKNLHQFIKLIGNND
                     FLVDNLNSAILIFDKNGKLKIKNRIATEIYKNLGFGYDIQDMHYNDLDIETNTFEDIL
                     IDENYDETNEVSIGNMFFKIKTIYIKDDEFKVCKIVQDISDLKRKEEELVLKTVAIRE
                     AHHRVKNNLHTVISIIKKQSRLSQNDEVKQCLDNIINRVFAILSTHYLLSKEVDNNIS
                     IREGINLLVSNIQGGYLFSKDIDIRITGEDFKISGEKATAILLVINEILQNCYDHAFS
                     DREHGTIQILINKDNDYRNIAIIDDGIGFDSNNINKNSLGTYIIDSYITQMLKGNIER
                     KSSKNGTEVLITIPTQINI"
     gene            14696..16129
                     /gene="eutA"
                     /locus_tag="KRL67_001039"
     CDS             14696..16129
                     /gene="eutA"
                     /locus_tag="KRL67_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004458698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase reactivating factor
                     EutA"
                     /protein_id="HBH1521938.1"
                     /translation="MREELLSVGIDIGTSTTQLIFSKLIVENTESNFSVPRISIVDKE
                     IIYKSKVYFTPLISNNEINGQQIKDIVENEYKKANISKEDIHTGAVIITGETARKENA
                     NDVLHTLSGFAGDFVVATAGPDLESIISGKGAGAHIYSKEHSTTIVNIDIGGGTSNIA
                     SFKRGEVTDTGCLDIGGRLIKIDKISKKITYISPKIDKIIKDNNLNLQVGTMATVENT
                     KPIVDIMVDLLMESIGLKKQTPLFDFIITNKSISIDENIKNVSFSGGVADYIYYDGEI
                     KDYFEYGDLGILLGQAIKNCEYLKKVNVVKSMETIRATVVGAGSHTTEVSGSTITYTS
                     EKFPLKNLPVLKLSIDEETGTSRQISEAIKNKLNWFKLENDLQKVAIAMIGKKNPTFL
                     DIQEYAKGFVDGMQQIIENDLPFIVIVENDMAKVLGQAIYALLNYEKEIVCIDSVKVE
                     NGDYIDIGKPIVEGKVLPVVIKTLVFN"
     gene            16147..17511
                     /locus_tag="KRL67_001040"
     CDS             16147..17511
                     /locus_tag="KRL67_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003454054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase subunit EutB"
                     /protein_id="HBH1521939.1"
                     /translation="MILKTKLFGRTYQFKGLYDVMAKANEEKSGDKLAGLAAESAEER
                     IAAKVVLSHIKLEDIRNNPAVAYEEDEVTRIIQDAVNEKVYEEIKNWTVSELREWLLD
                     SNTTSLDIRRISRGLTSEMIAAVAKLMSNMDLVYAAKKIKVTAHCNTTIGEQGTLSAR
                     LQPNHPTDDPDGILASLLEGLTFGIGDAVLGLNPVDDSVESVTKVLKRFEEVKQKYQI
                     PTQTCVLAHVTTQMEAIRNGAPADLIFQSIAGSEKGNEAFGFNGDTIEEARQLALKHG
                     TATGPNVMYFETGQGSELSSEAHHGIDQVTMEARCYGFAKKYEPFLVNTVVGFIGPEY
                     LYDAREVVRAGLEDHFMGKLHGLPMGVDVCYTNHMKADQNDMENLAILLTTAGCTYFM
                     GIPHGDDVMLNYQTTGYHETASLREMFGLTAIKEFDAWMEKMGFSKDGKLTELAGDAS
                     VLLG"
     gene            17524..18405
                     /gene="eutC"
                     /locus_tag="KRL67_001041"
     CDS             17524..18405
                     /gene="eutC"
                     /locus_tag="KRL67_001041"
                     /EC_number="4.3.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014952556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase subunit EutC"
                     /protein_id="HBH1521940.1"
                     /translation="MNEKDLKALVEQLVGQMVGELDTNVVSETVKKATEVVVDNNACI
                     DDITEVDIRKQLLVKNPKDAEAYLDMKAKTPARLGIGRAGTRYKTETVLRFRADHAAA
                     QDAVFSYVDEEFIKENNMFAVETLCKDKDEYLTRPDLGRKFSPETINSIKSKFGTNQK
                     VLILVGDGLSSAAIEANLKDCVPAIKQGLKMYGIDSSEILFVKHCRVGAMDHLGEELG
                     CEVICMLVGERPGLVTAESMSAYIAYKPYIGMAEAKRTVISNIHKGGTTAVEAGAHIA
                     ELIKTMLDKKASGIDLK"
     gene            18425..19078
                     /gene="eutL"
                     /locus_tag="KRL67_001042"
     CDS             18425..19078
                     /gene="eutL"
                     /locus_tag="KRL67_001042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003761789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutL"
                     /protein_id="HBH1521941.1"
                     /translation="MKNDVIRPNILGIKIISNISPEMAQKLELKSHHKSLGLITADCD
                     DVTYTALDEATKASEVDVVYAKSMYAGAANASTKLAGEVLGIIAGPSPAEVRSGLNAV
                     VDFLEYGATFISANDDDSIAYYAHCVSRTGTYLSEVAGIREGEALAYLVAPPLEAMYA
                     LDAAMKAADVKMCELFAPPTETNFGGALLTGSQSACKAACDAFAEAVKSVADNPIGF"
     gene            19089..19790
                     /locus_tag="KRL67_001043"
     CDS             19089..19790
                     /locus_tag="KRL67_001043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMC domain-containing protein"
                     /protein_id="HBH1521942.1"
                     /translation="MDAIGLIEVIGYVAAIEASDACLKSANVNIVRIDKVGAGIVTLT
                     ICGDVGAVKAALEAGEIAASRVGTLRTSHIIPRIHNEVTNALFKTKEPKICEVSEDKE
                     KNFEITLEITDNVSKNLSEKENVILDVTENSGDAEKLYSDIDKTNKKIETVDDSLTEA
                     NEEKIENTNDKEVLEQVKIENSDFDRDDLSKNSVKKLKAMAKKLDSSITYKDLNVLKK
                     EELINLVKKLSRRDR"
     gene            19793..21262
                     /locus_tag="KRL67_001044"
     CDS             19793..21262
                     /locus_tag="KRL67_001044"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetaldehyde dehydrogenase (acetylating)"
                     /protein_id="HBH1521943.1"
                     /translation="MTLLDKDLVSIQEVRTLVRRSKVAQEKLSKMSQEQIDKIVKAVV
                     DAAFSNATKLAKMANEETGFGKWEDKVIKNTFASMGVYETLKNTKTIGIIGEDKEANT
                     IDIATPVGVVAGLIPSTNPTSTVIYKTIISLKAGNTIVFSPHPNAKNCILETVNIISE
                     AAIKAGAPEDSILCITNPSIVGTNELMKHKDTNLILATGGEAMVRAAYSSGTPAIGVG
                     PGNGPAFIDKSANVKLAIKRIIDSKTFDNGVICASEQSIVIEKSMEETVLKELKNQGA
                     YILNEEESERLAKFILRANGTMNPQIVGKSVEVIANLADLTNVPKNTKVLVARENKVG
                     HNVAYSREKLTPILAFYVEENVEAIMNRCREILLNEGAGHTFSMHVNDEELVKRFAIN
                     MPVSRILVNTPSALGGIGASTNLVPALTLGCGAIGGSSTSNNIGPLDLINVKRVAYGI
                     KEIEELGRNKSQVEKSMFDSMDKEELINLLVQKLVKELA"
     gene            21348..21635
                     /gene="eutM"
                     /locus_tag="KRL67_001045"
     CDS             21348..21635
                     /gene="eutM"
                     /locus_tag="KRL67_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_464705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutM"
                     /protein_id="HBH1521944.1"
                     /translation="MASTNALGMIETKGLVGAIEAADAMVKAANVQLVGKEQVGGGLV
                     TVMVRGDVGAVKAATDAGAAAAERVGELISVHVIPRPHFEVDAILPKVSAE"
     gene            21762..22523
                     /locus_tag="KRL67_001046"
     CDS             21762..22523
                     /locus_tag="KRL67_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP--cob(I)alamin adenosyltransferase"
                     /protein_id="HBH1521945.1"
                     /translation="MGVLTENEVKRKIGKNKNNITEFYIEKGQIITPSAKSYLAERSI
                     ELKYVDKLSDVNNEPKEVVKEVVKEKKYKYTTVFGAKFDEKPEHMTQLNGNLLVFKDH
                     KRIILRGKIDSLESEILKVQILCQKLDMPKLVKELQEVLDFVRNIMRCEVLDEKLDDF
                     KLLGLTPQELREQSHYPKKYFGIGHEFPSYEMGEVVVAINSIRSNTREVELAAYEAFK
                     GEYGNVHREDLIKALNRLSSVFWIMIYKVRTNKYK"
     gene            22536..23165
                     /locus_tag="KRL67_001047"
     CDS             22536..23165
                     /locus_tag="KRL67_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate propanoyltransferase"
                     /protein_id="HBH1521946.1"
                     /translation="MENLLNEIVEEVITRVKKEAFIEVEASGRHVHLSREDVDKLFGE
                     GYTLTKLKDLSQPGQYACKERVTITGPKGSIKNVVVLGPCRNETQVEISLTDGSTLGL
                     KAPIKQSGDLEGTLSIKISTQYGEVELDKGLMVAKRHIHMTPKDAEKFNVCDKEIVEA
                     KVMSQRPLTFDDVVIRVSDSFKTYMHIDYDEANACGYSKGTVAKIIKKV"
     gene            23206..23931
                     /locus_tag="KRL67_001048"
     CDS             23206..23931
                     /locus_tag="KRL67_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02536 family ethanolamine utilization
                     protein"
                     /protein_id="HBH1521947.1"
                     /translation="MNYDNLVDLIMEEIYKKINSNEIKISNKPKAVIVFEQDNNKFNL
                     LKDEFEIVKFDKSIKECEIVIVSRLCMRGLSNIALGNSTSDEERFILKMIMKGKKVYV
                     LDEGIEYKKYKDTAPKALYKKFMSFEDEICKFGVEIIKDLNIITKNKLNIKKELESTR
                     GSKKEDTKVLDKDVLNLTSVESVKIDNEFSLDLRTKKLISEADLRKPTINGVKNILVN
                     KKSIITPLAVDFMRIHHLKLKKV"
     gene            23944..24216
                     /locus_tag="KRL67_001049"
     CDS             23944..24216
                     /locus_tag="KRL67_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EutN/CcmL family microcompartment protein"
                     /protein_id="HBH1521948.1"
                     /translation="MDIGKVVGNVWATRKDEKLCGQKLLVVKILKTKDTYKEGFMVAA
                     DNAGAGNGDLVLISKGSSARESIEKAHVPVDATIVGVVDSFEVFDE"
     gene            24209..24760
                     /locus_tag="KRL67_001050"
     CDS             24209..24760
                     /locus_tag="KRL67_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMC domain-containing protein"
                     /protein_id="HBH1521949.1"
                     /translation="MNKSIGAIEFKSIAKGIEVSNEMIKKSSVDVLYLKSICPGKFLI
                     IVGGETSYINECVDYGIKLGEGYIVDNFVINATSQEILDGFKNKYQKLDSIVSIGVVE
                     SSKVCAGIKMLDKTLKSGDLVLVKLQLSFAIGGKLVYIVAGDLSSLEYALKESENVVR
                     EKEVIYKTVIPSVDSQIIKSLIK"
     gene            24772..25863
                     /gene="eutH"
                     /locus_tag="KRL67_001051"
     CDS             24772..25863
                     /gene="eutH"
                     /locus_tag="KRL67_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization protein EutH"
                     /protein_id="HBH1521950.1"
                     /translation="MSINEIIIYVMVVFMVLGAIDKCIGNKFGLGEQFEEGIMAMGSL
                     AVAMVGVICLAPVLADVLRPIVVPVFNILGADPAMFAGSLLANDMGGAPLALELAKDP
                     NAGLFGGLIVGAMMGPTIVFIIPVALGIIEKDDQKFLATGILAGIITIPIGAFVGGLV
                     AGFELMMVLRNLVPIIIFAVVIALGLLKLENAMIKGFTYFGKGVVIVITLGLAAAIVE
                     ALTGIVVIPGMKPIGEGIEIVGDIAIVLAGAFPLVFVITKVFNKPLMALGKVLGMNDV
                     SAAGLVASLANCIPMFGMMKDMDNRGKIINVAFSVSAAFVFGDHLGFTAGFNANMITP
                     MIVAKLVGGITAVLLAMVIANKTLKKENV"
     gene            25865..26338
                     /locus_tag="KRL67_001052"
     CDS             25865..26338
                     /locus_tag="KRL67_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF861 domain-containing protein"
                     /protein_id="HBH1521951.1"
                     /translation="MDISNIDKNLIETLVRQIIEEKISETKDTVDFVRNKDISGITSI
                     KLPTVKVSESDRLDTGNPSDVVYTKDLFTLEESPRLGCGMMEMKETTFDWTLNYDEID
                     YVIDGTLDIIIDGRKVSASSGELIFIPKGSKIQFSVPDYARFIYVTYPADWASQN"
     gene            complement(26473..27522)
                     /locus_tag="KRL67_001053"
     CDS             complement(26473..27522)
                     /locus_tag="KRL67_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004452764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBH1521952.1"
                     /translation="MSLTKLFQSLYIYNESELFYKNYYFAKQNPESLKLFIHNLNEDY
                     IVNNNLIIPYIYNEKIPQFMKDETYFECSSKDNVILIKHNRYTPQFMHEHIFFEMIYV
                     LSGKCHQIISHHSIHMKEGDICIVSPGVKHSIGVFDDSIIINVLIRRSTFEDAFFDFL
                     RNNNLLSSFFLNSIYKNKHNDYILFHTNNDKSIEMSILEMFSEYSKQDRYYNNILNHL
                     IMVFFAKLLRGYENSIEVPNMTKKDNLKNTHILTYIQDNYFDITLEELANEFHFSIPY
                     CSKIIKEIAGCNFTQLLQRIRLERAESLLINTNISIADISNNVGYPNVEHFIRLFNKK
                     YNMSPSKYRKNTSKI"
     gene            27693..29051
                     /locus_tag="KRL67_001054"
     CDS             27693..29051
                     /locus_tag="KRL67_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBH1521953.1"
                     /translation="MTTLEVVNEKNKLGVWRQRIGYGSSDLACNLIWQMISLYLLYFY
                     TDVMGLSAVAISFMFVVTRVIDGVTDLLVGYCIDKTNTRWGKSRPYFLFGAVPFALFA
                     VLAFSVPKISSNGKLIYAYVTYIGLSLTYTIVNIPMASILPSLTDDTNERTTLSTSRK
                     FFGFLGSTIVSYSALTLVGKFGGTDEALGFRIVMMLFAFIGCAIFLFTFSNVRERVEV
                     KSENVTLSQTIKSLKENKPWKLFALNILFMWTGFFIQSSALIYYFSYNIGSKTLATTV
                     ATIMSIIPILANFLVPILAKRLGKRNLYITSATVQFIGLGIILIADLNTSLIIAGSIV
                     SAFGYGIKESIYFSMQADPVDYGIWKTGINTTGTLNSINGFLGKCAQAISGGLGGALL
                     AWGGYIAKAEVQPVSALVAIKVMYVYIPMILLICSMITMKFYDLDKQYPKIKADLDAR
                     NK"
     gene            29089..30657
                     /locus_tag="KRL67_001055"
     CDS             29089..30657
                     /locus_tag="KRL67_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 78 glycoside hydrolase catalytic domain"
                     /protein_id="HBH1521954.1"
                     /translation="MSSITNSIKDFKMEINQDFINKAERLKPALIEKLVKPKKIINIK
                     MGKNDWEVIDTKSIDEISNISLGKGDSICIDFGNHQVGYVSFKIKPIGSPPDAPAHLK
                     IKLGETLCEIGEDSSTYNGGISSSWIQEETTHIDILPDEINMKRRYAFRYLEIKVLDT
                     SPKYKVIVENVYCNFVSSVDMSEVDYLSNIDDDLIQIDKIALKTMQDCMQTVFEDGPK
                     RDRRLWVGDLRLQAITNYETFKNNDLVKRCLYLFAGLTQNKGQIGACLFIEPKLLVDD
                     TSLFDYSLFFISCLHDYYEATKDMEVLRELWAVAYLQAKLALERVGTNDVVYDSDDWW
                     CFLDWNEGLNKQAGAQGVLIYTLKQAHRLAIVLGDSEKKEELEYEINRVVQGAKNHLW
                     DEKQGFFVSGSEKQVSWISQIWCILADVFDKETNTKILDNLLDNPPAIDIVTPYAYHH
                     LVECLILNNRREKALECIRGYWGEMVKDGADCFWELYNPNDKFVSPYGSRIINSYCHA
                     WSCTPSYFIRKYFV"
     gene            30909..31430
                     /locus_tag="KRL67_001056"
     CDS             30909..31430
                     /locus_tag="KRL67_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HBH1521955.1"
                     /translation="MEEIVKKEQASMNDIFLYLKAKSNSLYKFVILYNEFINKPKDYG
                     TGELINMVEVHMLTAIEENPGISPTQLAKIWNRTKGAISQTISKLEKKGYITKEKKEG
                     NAKTILLYPTTSGVELSKAHKLYDTIDVTRTFEYLIKECTLEEIDSFYKVIDLFINLL
                     EESPQENNDSEKV"
BASE COUNT        12389 a         3828 c         5207 g        10079 t
ORIGIN      
        1 atattatact atcttttctt tttggaataa actatacttt aaatatagat atttaattta
       61 aataaatttt acagtattaa tagtaaatta tcttagaaag gaatgtgctt tatatgtcta
      121 cagacaaagt tgttggaatc atagatgaag aaacggcaga acttgcagga attgaatata
      181 ctggtaaaat ttatgcttct tctggtgtta taaaacatat aaagaaaaaa catagatgtc
      241 aactgagtaa ggatattttt aatgatataa tagatactat aaaaatggta ttaaagtcac
      301 ctgaatatat aggaagtcat cctaaaaaac ctggaaaaag tgtggagttt ataaaaaaaa
      361 ttgatgatta tattttagta gctacagaat tagatacaaa aaaaggatat ttatatgtat
      421 cttcaatgta tgctataaaa gaagcaaaat tagaaagtag agtaaacagt ggaagagtaa
      481 aacagtatga tggttaacat aatattatta attttcccta aaatattgaa aaatattatt
      541 atataagtta taatattaat atacacgtag atatttatta gttagatttg aggttggaaa
      601 aggcacccaa tacgctacta tagagtagtt aatagagatg caggatacgc cgccctgcca
      661 aatctaatta aattaaaaag agaggattaa ttcctctctt attgtttttt gagatactaa
      721 tttaatctat ataaaaagat tgaaggtcat ttatactaaa agttttcagt tatatacatt
      781 aaactaatta aataagatat ttataatcta tatgtgatac atattatata ttaataaaag
      841 aaaaatatat agaaaatcct tataaaaatt taaattgtaa tgaataggaa gtaattaaaa
      901 agaattttta ttgattttta aaaatttagt tagaaactat atcattatgg caaaaaatgt
      961 attataatgt ttagaaatat tataataagg agcgattaaa catgaatcta tcgaagaaaa
     1021 tagaagataa aagaaaagct aaacagagag ctgaaaaagt taagaaagct aaaattgcta
     1081 cagctggtgt ggtattagga gctgttactg gtgcagtgag tggagtactt ttggctccta
     1141 aatctggaaa agaaacaaga gaggatataa aagatgcttc acaacaaatt gctgaaaaaa
     1201 taaatatgaa aactgtagat gtaaaaggaa aagtatctga gaagctagag gataaaaaag
     1261 gaaactttat agaatcaaag aaaaaaataa aaaaatatat agataacaaa aagagtgttg
     1321 aaaattctag tgaggaagat aaaataatag atatagttga accagttagt gttactgagg
     1381 aataatatgg acaatacaat aacgattaac ataagtataa tatatgtatt attagctata
     1441 cttgcttgta ttgctttagt ttatttaatt attgtacttg ttaaactaca taagatttta
     1501 aataatgttg gaactatgtt aaatgaaaat actcaaaata taaatacaac tttaaataat
     1561 cttcctaatg ttgtagaaac ttttggagaa gtaggagaaa atatgaaaga tgtaacagaa
     1621 gtagttactg aagttacagc agattttctt gttactaaag acagtgtcaa atcaaatatg
     1681 gatgttatag ttgatatatt gactataatt aaaaatattt ttctagataa aaaataataa
     1741 gtttaatata aaataagata taatttaaac gaccttaaaa tagcatttat aaggtcgttt
     1801 tttattagct taatttgaca ctatgtgttt ttaataattg gtaatatttc tctttatcat
     1861 ttgaacatat atatctatgc ttgttgtttc aattacatcc ataagaacat ctttacattc
     1921 aagctcaaaa tcttccattc cttctaaata ttcatcctct gcatcaaaaa agatataaga
     1981 attatatagc cattcttcaa atttttcttc aagtacatcc cagtacgaac tccaaaataa
     2041 tccacaacct gacatataag atgctctact ttgatttata taagtctcct ttagaagctt
     2101 ttgtaattta ctgctataaa gaaaatctgc ttttccactg cttgatatta attcatttaa
     2161 atcttctatt gacattaatt caactttacc acttttatca taaggagtat aattttcatc
     2221 aacccattta tgtggtttgt tttctctgca tactgttgct atcaactcta caacttttgt
     2281 tcttatcatt tttttatcct aacttattta ttttttataa aataatcata attttataat
     2341 ttatctattt cttgtaatgc taatttaaca gtttcatatc tatcagcacc atttattact
     2401 acatcacctt ttacaagttt attagcttct ccacctacaa cataaacctt tttacctaaa
     2461 ccttgcttat aactagacgc ctcaattatt aaagaatctt tcaattgcca atgtaaaatc
     2521 tgagcagcca ctttatcagc atcacctgta taaacgatta cattttcata cactttattt
     2581 tcccctcccc cttgagaatt tgatatagat ttgtttagta taccttctgc tatcaattta
     2641 gcaactttat ctttattatt aacataataa tctgtatctg ctttactatc tacaaaacat
     2701 acttctatta atattgctgg tgcaatagta tgattaagcc aataaaggcc tctaacatca
     2761 gactttgcac ccctattttt gaatactgta gctaacctag tattcactct ttctgcatag
     2821 gttttgccat tattagtctt atatattgtt tctgtcccta ctggagttga agttgtgaca
     2881 tttgaattaa aatgaatctg aatagccaaa tcagtttcct gtttatttgc tatagcacat
     2941 tggtctgcta aatgattact tgattcatca actcttcctg tataaacagt agctccacct
     3001 attttaagcc atttaacaat taagtcattc agtattcttg tttctttgct ttcgtttata
     3061 tacccaacag ctcctgtacc ttttccaatt aaagtgtgcc ctggtattat tactactttc
     3121 aaactaacat ctcctttaca attaaattta acttctattt aatatatgca taacctagaa
     3181 ttttgtttct ttttatatct tatactttta ttataaccca ttttgtaaaa atatagaata
     3241 ttaagtaaat aatgttaaaa ttataatttt gtatataaaa cattacttga ttgcaataat
     3301 tttatagcat attcatctat tctatatcca gttctataat atatatgttt aattccagct
     3361 tgaattatag ccttagtaca atttaagcaa ggaaagtgag ttacataaat agagcaatct
     3421 ttaactgaaa taccttcttt tgcacaataa taaagggcat tttgttcagc gtgaatggtt
     3481 ctaatacaat gtccatctct catttcatga ccaacttcac tacaatgctt atctccagaa
     3541 atactaccat tatatccagt agatactatt ctgttttctg aattgacaat tatagctcct
     3601 acgtaagctc tatcacaagt tcctctttca gctactgttt cgcacagtct cataaaatat
     3661 tcttgccatg agcatctatt ttcaagtttt ttattcaaat catatacctc caaaatttat
     3721 atctaatata taggatttat accttaaaaa taatattaaa aacaaattta tacgataaat
     3781 aaaatttatt aatactgttt ttattctaaa ttattttact ttaaattaaa gttacaagga
     3841 atctaaaatt atatatatct ttctttgtaa tatgaatgtt ataatataaa aaaactgtct
     3901 caataaaata gagacagttt tacagtcaaa gtttataata caagttcata tttttcttta
     3961 aattctttta cagcttcggg ccatgccttt tgttctgtaa tacctttttc tttagctatt
     4021 ttaattgcaa tctctcttat cattgcagcg ccttctaata ttcccatttt taatctccct
     4081 tcaaatatct ttttgtatag aatataaata gtgctaagat ttattacatg ctgcatatac
     4141 aatatctata caacacataa cttataaatt ccttttattt tcatacgtct ttaaccgaca
     4201 tataattata ttcaataagt tttaaaatta tgaactcaat tataattatt tttatttttt
     4261 tagaatctaa acactaaaaa ttgtaggtaa atttgtctta tgcctttata aatatttcta
     4321 cagttttaat tattgtacat atctattttt attataaaaa atgcttaaaa atcgagaaca
     4381 tacgcataaa cactgtatat aagcaaaacg gttaaaggaa aaagaagaaa tttacagaat
     4441 ggttcgaaaa gcccactcct ttaggggtgg gatggatagc ataaatagtg tataaaaagt
     4501 attgatacat acacatataa tgttaatata agtatatgga gaataaatat agaaaaactc
     4561 aaacaaatgt cagtttaatt aattatcact ttgtattctg tcccagatat aaaagaaaaa
     4621 tatttaatat agaaggctta gaagataggg tattcaaaga gattgctatt ctgcattttt
     4681 taataatgaa tgttaatgat gatttaaagt caatcaacag agaattgtgt gttaaaacat
     4741 atgataactt taaaatatta catgataaag aaatagatag gctaaaagaa ttaaaattaa
     4801 atggttataa attaatatct agtatgggga tataaaaact ctatatagat tgggaaacga
     4861 gcctaatatt aatgttaatg gtgtcaacag atactttgtt aatagaagtc ttagggaata
     4921 tgattagtga taatatattg taagttatga tttattataa ctgagagtat tgtagaaatt
     4981 catgtagtat ataaaaaccc tacaccttta agtgtgggag tttcagttga atgttaaaat
     5041 gcgttgcttg aagcaacggg tagttttgcc tataatagcg ttaacaactg aaagaaaggt
     5101 ggttatccct aatgttaagc gaaaacatta aaacaatcag gaagtcaaaa ggactttcgc
     5161 aacaagaact tgctataaaa ttgaatgtag tacgacaaac aatatctaaa tgggagcaag
     5221 gattgtcggt tcctgattct gatatgttga tttccatatc agaggtactt gaaacgccgg
     5281 taagcacttt gcttggagaa actattgttg agtcggaagt tgataactta aaagtgattt
     5341 ctgaaaaatt agagattata aacttgcaac ttgcacaaag aaaaaacatg agaagaaaaa
     5401 tgtttcattg gttatttatt ttattatgtg cagtcatagt aataggtttt ttggtcttag
     5461 ggggactaaa tagtccttat ttggattgga attatactga ccctgaaacc gctgtttttg
     5521 gagtagcttt tcatgcgttt gagtggctat ttgtcagatt agcaccgatt gtccttatag
     5581 gtgcaattat tggaagcttt ttgacacgta agaaaatatg aagacattct gctttttata
     5641 atacagcaag cgttacttaa aagcgtcatt ctacatgaca tcgaggacga ttatgtaagg
     5701 aagaatcttt ttgatttcca actaagcgaa gtcattgagg acgacaacaa gcccaaaacc
     5761 gtattaagtg aagtacagga agcaagactg ctagacttat acgcccatgc gtcagaggca
     5821 aagaaatgct aagtctgata gcatgattta caacgatttg tacaacgatt gcgagtagaa
     5881 atatattata aaattagaaa agaaatgact tgataattta tccatgttat aattattgat
     5941 gtgaaattaa taaatcgaaa ttcctttttt agatttaaca atactgagta aggaaaaagt
     6001 gaatgacttt tgcaaggagg tgttaatatt agacaagtaa agtcttttgt ttttccagtt
     6061 atatccttaa tattcttaga ccaaattagc aaagttctta taggattatt cttaatagac
     6121 tttgaaattg atataattgg gaaattttta agattcaatc ctgttcaaaa tacaaatctc
     6181 tcttatgggg gaaactttat tggtattcta tctaatttat gggtattggt attgtttaac
     6241 attttagtta tcctagttat tatatctgga tatgcttttt acaaatcaaa aaatgaacaa
     6301 acgagctatt cagtaaaagt aattatgtct tgtggacttg ctggtacaat atgtagcttg
     6361 atagataaat tattttgggg aggaagttta gattttttgc agataccaag cttttttatt
     6421 ttcgacttaa aagactgcta tcttactgtt gcagaaataa tatttgttat cataggaatt
     6481 ttacatagta gagaaatatc aatgaaagaa tacatatatt tttgctatcg tcagtttaaa
     6541 agataatttt agaagataaa tttaagtttc aaggtaaact atttaatcac agcaaatagt
     6601 tggctcactt cagcttttgg gctaaactgt gagcactcat taggtctctg ctatctaaca
     6661 aacggagcac aaaacggagt acaaaaatga aaattaaata ttgaatataa aacacagtta
     6721 attttgatta aaaagttggc tgttttttct tttgtagaaa tttaagcata ggtaataaaa
     6781 aaataactgt atttatgtat aattggaggc aataaaaagc cagtaaaacc cataaaatag
     6841 ggtcatactg gcaacatggt aagtttataa gaagataatc aaaaatttac gctttatttt
     6901 aatttaaaaa aatgattact ttaaagaaat cattttttat tataatcact gagacatcaa
     6961 atattctaaa taaaattttt acttatctgg aattgtatat tttttctata atattaacta
     7021 cagcgtacat tgcccatgca cagatagcca atacaaatac tcccatcatt actaaatcta
     7081 atttaaatac ttgagaacca tatactatca agtagcctat tccatatctt gaaactaaaa
     7141 attctccaac tattacacct acccatgcca taccaatatt aattttagtt aaatttatta
     7201 aatttcctgt gtttgcaggt aatatgagtt taaataatat ttgagattta ctagctccaa
     7261 aactctttaa catcttaatt ttttcttcat ctatattcat aaaataatta taagctgaaa
     7321 gtatagtaac aacaacagaa atagttacag ctgttacgac gattccttct attccagcac
     7381 ctacccaaac tataattata ggagctaaag cagtcttagg aagagcattt aaaactacca
     7441 aaaatgggtc taatattttt gataattttt ctgaccacca aagagcaatt gctaccaaaa
     7501 tacctaatac agtacctata accagaccaa gagcagtttc atatacagat attccaatat
     7561 gtttaaataa ttgaccacta aatgcatatt gaataaataa attgtaaata tcacttggtt
     7621 tgctaaatag aaatgtattt ataacattta agtcagctaa aagttcccag attacaataa
     7681 aaccaattaa aattaatagc tggtagaata atacatttct tttttctctt tttacacctt
     7741 taacatagtt tatatatcct tcagatcttt tattaagcct catgtgtatc tagctccttc
     7801 cataatatgt cgaaatactt ttgaaaatca ggaactttac gtgttttgag aggcgttttt
     7861 gttccatcca tgttaagttc aatatcgtaa atatttttta tggtagctgg tcttttagat
     7921 agtattgcaa ccttatctga tatgcttatt gcttctacta tagctaaaaa agtgcaacta
     7981 attgcttaaa gaaacagaat tatccatttc agcacccata taaacaatat taagatttcc
     8041 taaactgcta tcccaaacga tactattaat aatagtagat aataacaatc tcttatcatc
     8101 taaactggca ttatcaactt catcattaaa tctcttcaaa ttactaacaa taatatcaat
     8161 attaatactg tcactactaa tattgatgtt actagattcc aaagtattaa tcttatcctt
     8221 taaaatacta atctcattat ttaagctctc aatctgggaa actatataat cagaagcagt
     8281 actaccagta ttttttgcaa gctctataac taaattttta ataagtcttt ccttatctaa
     8341 aatttgattt ttaagcaaat tagtctcaga tttaaaagca ttaaaaacat tatcagcctt
     8401 aatgttatta ctattgaatg cacttataat agactcttct ttacaattct taattttatc
     8461 tataactaaa ctttcaacta atggaccatt aaggttttta cagttacaac tatcagaccc
     8521 aagtgttttt ttagtaccac aaatataata atactttaaa tccttgtcag tttttttgcc
     8581 tttataggta attctcatat tagaaccaca tttaccacat tttataagac cagataaaag
     8641 agcagttttt ccagttgata tccttggggt tttatcacga tttttatcta attgattttg
     8701 tacttcaagc catatttcag gttctatgac tccatggtgt tttgcaattg ctgctattga
     8761 gttagctgta tttttagcat aggtaaggat tccacatttt ctatctggag tacctacaac
     8821 atccatacca ctatttttaa ggtaatctaa aatttcatca ttagctttta catatgctgg
     8881 atttctaagt attctagata aagaactagg gtcaaattta ccaccacgag gaccccttat
     8941 tccattatta tagatatatt tatataactt agataatgac ctaagctcta agtatttatt
     9001 gtatattagc tttacaacct ccatttgttc attatttact tgaagcttat acatacttct
     9061 ttcctttaaa ttttcatcta agaatgtgat tttttcgctg ttaaatccat gaggtgtatt
     9121 gcctccaagc catctaccac tacgggctaa ttcatacata ttgtctttta tacgttctgc
     9181 tattgtttct ctttctaatt gagcaaatac acttgatata aacatcatag ctcttcccat
     9241 tggagttgaa gtatcaaatt gttctttgat agatataaat gatatattta gttcatttaa
     9301 tttttctatt aaagtagaga agtcagatac atttctcgat atcctatcta atctataaca
     9361 aactaggtaa ctaaatttct tatctttggc atcctgcatc attttcttaa attgtggtct
     9421 atctatattg ctaccactaa atccttcatc ttcatatatc aaaaaatctt ttacaccaat
     9481 agactttgca taatcaatac ataattgtat ttgattttct atagaatcac cttttccagt
     9541 aaatcttgat tttcttgaat aaattgcagc tttcaaaatt aaaacctcct ataagtcagg
     9601 atatatattt aaaaattatt tatatacagc tttttaagta aacttattag aatgaattta
     9661 ttaattaggt aaatttatat aattctactt tttataatgt atgactattt attttatttc
     9721 tttactattc aagttctaaa tcaataataa attttgctat ttaattgtat caggacaaat
     9781 ctctaatttc atagattatt aatataaaat catagattag tgtgaaaagg ttgaaattta
     9841 aatattttaa aagataattt tttggaacta ataggtataa caaaaattta tagggggatt
     9901 gcatatgaaa atcaatgtta tagaaatttt ccctaaagat aaagctaaac ttaataaaat
     9961 agaaatggat aaagctagtt ggtttgtaaa tataataagt aaaaaatatc ctaaagaagc
    10021 tttaaatgaa gcatttagta ctttagaaaa agaattaaat ataagtaaag ctaatacata
    10081 agctttgatt attgatttta tatactattg attttataca ctatgacttg acattatatt
    10141 aatcaaatag tatattttta aatatagcac aatgaagtgt taaaagagca atggagctcc
    10201 atagataaag tctatggagc ttttttgcgt atttaactta aggaggatag aactttgaat
    10261 aactttgaga ttaaaactag aataaaattt ggagaaggtt cacttaaagc tttacaagaa
    10321 ttaaaaaaca aaaatgtact aataataaca gaccctttta tggtgaaatc aaaaacaata
    10381 gataaaataa catctaattt aataaactca aaatatcaag tttttgaaga aatagtacca
    10441 gacccaccaa tagaagtagt agtagcagga atagaagcat ttaaatctat aaatccagat
    10501 gtaataatag cattaggtgg tggttctgca atagatgcag caaaatctat aatggacttt
    10561 tctaaaaaaa tattaaaaat taacgaagta gagtttatag caataccaac aacaagtgga
    10621 acaggttcag aagtaacatc atttgctgta ataacagata gtcaaaaaaa atgtaagtat
    10681 ccattagtga gtgatgattt acttccagat atggcaatac tagacccaga gcttgtaaaa
    10741 actgtaccaa attttataac agcagataca gggatggatg tattgacaca tgctatagaa
    10801 gcttacgtat ctacaaattc taatgacttt tcagatgcac tagctgaaaa agctataaag
    10861 ttagtcttta aatacttaat aaaagcatat aaagatggaa atgacataga agctcgagaa
    10921 aaaatgcaca atgcatcttg tttagcaggt atggcattta accaagcatc acttggcata
    10981 aatcattcta ttgcacatgt attaggtgga aaattccata taccacatgg aagaacaaac
    11041 tctatattac ttccccatgt aatagaatat aattcatcaa tagtaggata tactaacaca
    11101 acttactctg atacagctaa aaaatatgca caaatagcag aattagtagg atttgggaat
    11161 ggtaatataa gaatatctgt taggaattta gtaaatgaaa taaagaaact acaaaaagaa
    11221 atgaatatgc caactaaatt gacacaatgc aaattaagtt tagaagatat aactaatgaa
    11281 gaaagagaaa tagcaaaact tgctataaaa gatacttgta cattaactaa tcctaaggta
    11341 ccaactgaag atgatataat aaacatatta aataacatta aataactaca ttttattaca
    11401 tgtaaatata atattataca aataaggaaa atgtttttaa aaatataaat tatatattga
    11461 caaatataaa acataaaact ataatgaaag tatcacaaag gcgtgatact ttagtagaaa
    11521 gcaatggagc ttcttaggaa tattaatact attcttatgg agctcctttt attttaagca
    11581 aatagctttt tagtaaacta tattcaaaga aagcagggaa taaatttgac tgaagaatct
    11641 aagcaaagag ttattcaaga atatgttcct ggaaaacagg ttacattagc acatataata
    11701 gctaacccta atgaagatat atataagaag ttaggattag tacttgataa aaaagatgct
    11761 ataggaatac ttacaataac accaagtgaa gcgtcaataa tagcagcaga tgttgctaca
    11821 aaagcttcaa atgtatcttt aggttttata gatagattta gtggttcagt agtaatatca
    11881 ggtgatgtaa gttctgtaga atcagcgtta aatgatgttt tagaagtctt aggaaatatg
    11941 ttaaacttct catctacaaa aataacaagg acataataat acaatgaaaa atataatatt
    12001 tatgggaaag acaggtagtg gtaaaactac cttatgtcaa aaattagacc agttagaatt
    12061 aaagtataaa aaaactcaat cagtggaact ttataataat gcaatagaca ctcctggtga
    12121 gtatatggaa aatagaaacc tatacaatgc cttaataaca acatcagcag atgctaaaat
    12181 tatagcaata gtgtatgatg ccacacaaga agaaaattat atagcaccag gttttgcaag
    12241 tatgttttgc aaagatgtaa taggaattat aactaaaata aataaagcta ctgaaaacga
    12301 ggtcaatata gcttatgaaa gactatcact agcaggagca agtcaaatct ttaaaataga
    12361 cacagttgat aatattggac ttgaaaaatt atttaaatat ctagaaaaat ctaaaaatat
    12421 agatgaagtt taaggggaaa tattttttta ttcaaaaata ttattaaagt atgataaaat
    12481 aaaaaaatgt ataaactatt ttaagaagat ttaatcttta agcgaggtag gaacatgggg
    12541 aaaaaaatac tattggttga tgatgaacca ctgttaagga tggatacaaa agatatacta
    12601 gaaagtcatg gctatgaaat aataggagaa gcaagtgatg gatttgaagc tgttgaaatg
    12661 tgtaaaaaac acaaacctga tttagttata atggatatag agatgccaat acttgatggt
    12721 ataaaggcag gcaaaataat atgtaaagaa aatctagctg gtggagtact tttattaact
    12781 tcatttgaag ataaagaata cgtagaaaag gctaaaagta taggtgctta tggatactta
    12841 gtaaagacag caagtgaaaa atcgcttata ccaactatag aaatgtgttt aaataaggta
    12901 aatgaatttg aaaaaatgaa aaaagattta gaaaaagtaa attctaaatt gaatgaaaga
    12961 aaattagtag aaagagcaaa aggtatactt ataagagagt ttgagatttc tgaggatgaa
    13021 gcatacacta gaataagaaa attaagtatg gataaaagaa ctacaatgat ggaaattgcc
    13081 aaaatgatta taataggata cgatgactaa tacgataaaa aaaatatcaa ctgcacaaac
    13141 atacttaaat aataacgaaa ttgaaaaaat tatagaagta tcaaaatcct tagatttaat
    13201 ggcttctttt tatgaggctg atgtttttat agatgtacta agtaaatttg atgatgaagc
    13261 aattgttgta gcacatggta gacctaaaga aaagtcaatg tactgtgaaa atgttatagg
    13321 taaaaaggct ttaattgaaa atgaaccagg agttataagg tgcttaaaaa caggagaaag
    13381 agtaagagat attaaagctc taacacaaga gtataaactg gtcaaacaaa atattcagcc
    13441 aataacacta gatgacaaag ttttagcagt attaatagtt gaaaaagata taagtagtga
    13501 agttaagaat gagtttaatg ctagtgaaga cgaagaaaat aaaaatttac atcagtttat
    13561 aaaacttata ggaaataatg actttttagt tgataattta aattcagcca tattaatatt
    13621 tgataaaaat ggaaaattaa aaattaaaaa tcgtattgca actgagatat acaaaaatct
    13681 tgggtttgga tatgatatac aagatatgca ttacaatgat ttagatatag aaaccaacac
    13741 ctttgaagat atattaatag atgaaaatta tgatgagaca aatgaagttt caataggtaa
    13801 tatgtttttt aaaataaaga ccatatacat caaagatgat gaatttaagg tttgcaaaat
    13861 agtacaagat ataagtgatt taaagagaaa agaagaagaa ttagttttga aaacagtggc
    13921 tataagagaa gctcatcata gagtaaaaaa taatcttcat acagtaatat ctataataaa
    13981 aaaacaaagt agattatctc aaaatgatga agtaaagcag tgtttagata atataataaa
    14041 cagggtattt gcaatacttt cgactcatta tttactatct aaagaagttg ataataatat
    14101 atctatacga gaaggtatca atttattagt gtcaaatatt caaggtggct atttatttag
    14161 taaggatata gatatacgta taactggtga agattttaaa attagtggag aaaaagcaac
    14221 agcaatactt ttggttataa atgaaatact acaaaattgt tatgaccatg cattttcgga
    14281 tagagaacat ggtactatac aaatattaat aaataaagat aacgactata gaaatattgc
    14341 tattatagat gatggaattg gatttgatag taataatata aataaaaata gcttagggac
    14401 atatataata gatagttata taacacaaat gttaaaagga aatatagaga gaaaaagtag
    14461 taagaatggt acagaagtat taattactat acctacacaa attaatattt aaataataac
    14521 tcatctacaa agtagtagtt ttaattgagc aaagaggctt aaggttatta atagattaag
    14581 ttaattgaat atatttataa aaattataaa tatatattca atttaacaga tatttattat
    14641 aactttaagc ctctttgctt gtttaagaca tacttaaatt ttaggaggtg agaaaatgag
    14701 agaagagtta ttaagtgttg gtattgacat tggaacaagt actactcagt taattttttc
    14761 taaattaata gtagaaaata ctgaatcaaa tttttcggta cctagaataa gtatagtaga
    14821 caaagaaatt atttataaaa gcaaagttta tttcacccct cttatttcaa ataatgaaat
    14881 aaatggtcaa caaataaaag atatagttga aaatgaatat aaaaaagcaa atatatctaa
    14941 agaggatata catacaggag cagtaataat aactggtgaa acagctagaa aagaaaatgc
    15001 aaatgatgta cttcatactt tgagtggatt tgctggggac tttgttgttg caacagcagg
    15061 acctgattta gaaagtataa tatcaggaaa aggagctgga gctcatattt attcaaaaga
    15121 acattcaact acaattgtaa atatagacat aggtggggga actagtaaca tagcctcatt
    15181 taaaagggga gaagtaactg atactgggtg tttggatata ggtggaagac ttataaagat
    15241 agataaaata agtaaaaaaa taacttatat atcacccaaa atagataaaa tcataaaaga
    15301 taataattta aatcttcaag taggtacaat ggctactgta gaaaatacaa agccaatagt
    15361 ggatataatg gttgacttat taatggaaag cataggatta aagaaacaga cacctttgtt
    15421 tgattttata ataaccaata aatcaataag tatagatgaa aatattaaaa atgtttcatt
    15481 ctctggtgga gtagctgatt atatttacta tgatggagaa ataaaagatt attttgaata
    15541 tggtgacttg ggaattttac ttgggcaagc aataaaaaat tgtgaatacc ttaaaaaagt
    15601 aaatgtagtt aaatctatgg aaacaataag agccacagtg gttggagcag gttctcatac
    15661 tactgaagta agtggaagta caataactta cactagcgaa aagtttccac ttaagaactt
    15721 gccagtttta aaattatcaa ttgatgaaga aacaggtact tcaagacaaa tcagtgaagc
    15781 tataaaaaat aaacttaatt ggtttaaact agaaaatgat ttacagaaag ttgctatagc
    15841 catgatagga aagaaaaatc caaccttttt agatatacaa gagtatgcta aagggtttgt
    15901 agatggaatg caacaaatca tagaaaatga tttacctttt atagtaatcg ttgaaaatga
    15961 tatggcaaaa gtgttaggtc aagcaatata tgcattatta aattatgaaa aagagattgt
    16021 ttgtatagac agtgtcaagg ttgaaaatgg agactatatc gatattggaa aacctatagt
    16081 tgaaggtaaa gttttacctg tagtaataaa aacattagtt tttaattaat agaaggaggt
    16141 aagtaaatga ttttaaagac aaaattattt ggacgtactt atcagtttaa aggcttatat
    16201 gacgtaatgg ctaaagctaa tgaagaaaaa tcaggagata aattagcagg tcttgcagca
    16261 gaatcagctg aagaaagaat agcagcaaaa gttgttttat cacacataaa attagaggat
    16321 ataagaaata atcctgcagt agcatatgaa gaagatgaag taacaagaat aatacaagat
    16381 gctgtaaatg aaaaagttta tgaagaaata aaaaattgga cagtatctga acttagagaa
    16441 tggctattgg attcaaatac tacaagtctt gatataagaa gaatatcaag aggattaact
    16501 tctgaaatga tagcagcagt tgcaaaatta atgtcaaata tggacttagt ttatgcagct
    16561 aaaaagataa aggttactgc tcactgtaat acaactatag gagaacaagg aacattatca
    16621 gcaagacttc aacctaatca tccaacagac gacccagatg gaatacttgc atcacttctt
    16681 gaaggtttaa cttttggtat aggagatgca gtacttggat taaatccagt tgatgactct
    16741 gttgagagtg ttactaaagt attaaaaaga tttgaagaag taaaacaaaa atatcaaata
    16801 ccaactcaaa catgtgtatt ggcacacgtt actacacaaa tggaagcaat aagaaatggt
    16861 gctcctgcag atttaatatt ccaatctata gcaggttctg aaaaaggaaa tgaagcattt
    16921 ggatttaatg gcgacactat agaagaagct agacagttag ctttaaaaca tggtacagct
    16981 acaggaccta acgttatgta ttttgaaact ggacaaggtt cagaattatc ttcagaagca
    17041 catcatggaa tagaccaagt tactatggaa gctagatgtt atggatttgc taaaaagtat
    17101 gaaccattct tagtaaatac agttgttggg tttataggac ctgagtactt atatgatgct
    17161 agagaagttg taagagcagg acttgaagac cactttatgg gtaaactaca tggcttacca
    17221 atgggtgttg atgtttgtta cactaaccat atgaaggctg accaaaatga tatggaaaac
    17281 ttagctatac tattaacaac agcaggatgt acgtatttta tgggaatacc tcatggagat
    17341 gatgttatgc ttaactatca aacaacagga taccatgaaa cagcatcact tagagaaatg
    17401 tttggactaa cagcaataaa agaatttgat gcttggatgg aaaaaatggg attctctaaa
    17461 gatggtaagt taacagagtt ggcgggagac gcatctgtat tattaggata ggaggtattt
    17521 aatatgaatg agaaggattt aaaagcattg gtagaacaat tagttggaca aatggttgga
    17581 gaattagata ctaatgttgt atctgagact gttaaaaagg caactgaagt tgtagtagac
    17641 aacaatgctt gtatagatga tataacagaa gttgatataa gaaagcaatt actagtaaaa
    17701 aatcctaagg atgcagaagc ttatttagat atgaaggcaa aaacacctgc aagattaggt
    17761 ataggaagag ctggtactag atataaaaca gaaacagttt taagatttag agcagaccat
    17821 gctgctgcac aagatgctgt attctcttat gtagatgaag aatttataaa agaaaataat
    17881 atgtttgcag tggaaacttt atgtaaagat aaagatgaat acttaacaag accagatttg
    17941 ggaagaaaat tttctccaga aactataaat agtataaaat ctaaatttgg tacaaatcaa
    18001 aaggttttaa tattagttgg agatgggctt agctcagctg caatagaggc aaatttaaaa
    18061 gactgtgtgc cagctataaa acaaggtcta aaaatgtatg gtatagattc aagtgaaatt
    18121 ttatttgtta aacattgtag agttggtgct atggaccact taggtgaaga attaggttgt
    18181 gaagttatat gtatgttggt tggagaaaga ccaggattag ttactgctga atcaatgtca
    18241 gcatatatag cttataagcc atatatagga atggcagaag caaaaagaac agttatatca
    18301 aacattcaca aaggtggaac aactgcagtt gaagcaggtg ctcatatagc tgaacttata
    18361 aaaactatgt tagataaaaa agcttcagga atagacctta aataaagtcg agggagtgta
    18421 gaatatgaaa aatgatgtaa ttcgtcctaa tatactagga attaaaataa tttcgaatat
    18481 aagtccagaa atggcacaaa aactagaatt aaaatcacat cacaaaagtt taggcttaat
    18541 aactgcagac tgtgatgatg ttacgtatac agctcttgac gaagcaacca aagcttcaga
    18601 ggttgatgtt gtatatgcaa aatcaatgta tgcaggtgct gcaaatgctt caacaaaact
    18661 tgctggtgaa gtattaggaa taatagcagg accaagccca gcagaagtaa gaagtggtct
    18721 aaatgcagta gttgattttc tagagtatgg agcaactttt ataagtgcaa atgatgatga
    18781 ttcaatagca tattatgctc actgcgtatc aagaacaggt acataccttt cagaggtagc
    18841 aggaataaga gaaggtgaag cattagctta tttagtagca ccaccacttg aagcaatgta
    18901 tgcactagat gcagcaatga aagctgcaga tgttaagatg tgtgaattat ttgcacctcc
    18961 aactgaaaca aactttggtg gagcattact tacaggttct caatcggctt gtaaagcagc
    19021 atgtgatgca tttgcagaag cagtaaaatc agttgcagac aatccaatag gattttaagg
    19081 atagttagat ggatgctata ggcttaatag aagtaatagg atatgttgct gcaatagaag
    19141 cttctgatgc ttgtttaaaa tctgcaaatg ttaatattgt aagaattgat aaggttgggg
    19201 caggtattgt tactttaaca atatgtggag atgtcggtgc tgtaaaagct gctttagaag
    19261 caggtgaaat agcagcttca agagttggga cattaagaac atcacatatt ataccaagaa
    19321 tccataatga agtaacaaat gctttattta aaaccaaaga acctaaaatc tgtgaagttt
    19381 cagaagataa agagaaaaac tttgagatta cattggaaat aactgataat gtatcgaaaa
    19441 atttatcaga aaaagaaaat gtaattttag atgtcactga aaacagtgga gatgctgaaa
    19501 aattatatag tgatatagat aaaacaaaca aaaaaattga aacagttgac gatagtttaa
    19561 cagaggctaa tgaggaaaaa atagaaaaca ctaatgataa agaagtattg gaacaagtta
    19621 aaatagaaaa ttccgatttt gatagagatg acctatcaaa gaatagtgtg aaaaaactta
    19681 aagctatggc taagaaatta gattcttcaa taacttataa agatttaaat gttcttaaaa
    19741 aagaagaatt aataaactta gttaaaaagc ttagcagaag ggatagatag ttatgacact
    19801 tttagataaa gatttagtat ctatacaaga agtcagaact cttgtaagaa gatctaaagt
    19861 ggctcaagag aaactttcta aaatgagtca agagcaaata gataaaatag taaaagctgt
    19921 tgtagatgca gcatttagta atgctactaa attagctaaa atggcaaatg aagaaactgg
    19981 atttggaaaa tgggaagata aagtaattaa aaatactttt gcatctatgg gagtttatga
    20041 aactttaaag aatacaaaaa ctattggaat aattggtgaa gataaagaag ccaatacgat
    20101 agatatagct acaccagttg gagttgtggc aggtcttata ccttcaacaa acccaacgtc
    20161 aacagtaata tacaaaacta taatatctct aaaagcagga aatactatag ttttctctcc
    20221 acatccaaat gcaaaaaact gcatacttga aacagttaat ataataagtg aggctgctat
    20281 aaaggctgga gctccagagg attcaatatt atgtataact aatccaagta tagttggaac
    20341 taatgaattg atgaaacata aagatacaaa cttaatacta gcaactggag gagaagcaat
    20401 ggttagagct gcttactctt caggaacacc agctattgga gttggaccag gaaatggacc
    20461 agcatttata gataaatcag caaatgttaa attagcaatc aaaagaataa tagattctaa
    20521 aacttttgat aatggtgtta tatgtgcatc agaacaatct atagtaatag aaaaatctat
    20581 ggaagaaact gttttaaaag agcttaaaaa tcaaggtgca tatattttaa atgaagagga
    20641 aagtgaaaga ttagcaaaat tcatattaag agctaatggt actatgaatc cacaaatagt
    20701 tggtaagagt gttgaggtaa tagcaaattt agcagactta acaaatgtac ctaaaaatac
    20761 aaaagttcta gttgcaagag aaaataaagt tggtcacaat gtagcttatt caagagaaaa
    20821 acttacacca atactagcgt tctatgttga agaaaatgta gaagctatta tgaacagatg
    20881 tagagaaata ttgttaaatg aaggtgcagg tcatacattc tcaatgcatg taaatgatga
    20941 agaattggta aaaagatttg ccattaatat gccagtatca agaatacttg tgaatactcc
    21001 ttcagcactt ggaggaatag gtgcatcaac aaaccttgta ccagcactaa cacttggttg
    21061 tggtgcaata ggtggaagtt caacttcaaa caatatagga ccacttgatt taataaatgt
    21121 taaaagagta gcttatggaa tcaaagaaat agaagaactt ggaagaaata aatctcaagt
    21181 tgaaaaatca atgtttgact caatggataa agaagaatta ataaatcttc ttgttcaaaa
    21241 actagtcaaa gaactagcat aacgaaaata aacaatgaaa cttatggcta tcgctataaa
    21301 tttctaaaaa taaaaactat aaaattaaat ttttggagga taagattatg gcaagtacaa
    21361 acgcattagg aatgatagaa actaaaggat tagtaggagc aatagaagca gcagatgcaa
    21421 tggttaaagc tgcaaatgta caattagtag gaaaagagca agtaggtgga ggattagtta
    21481 ctgtaatggt aagaggagat gttggagctg ttaaagctgc tacagatgca ggagctgcag
    21541 ctgccgagag agttggagaa ctaatatcag ttcacgttat accaagacca cactttgaag
    21601 tagatgctat attaccaaag gtatcagctg agtaatataa aaataatttt aaaatttaaa
    21661 ttttgattga aaaagaggct atctcaaaaa tgagtcttac taataaactc aatgtatttt
    21721 gaggtagtct tttttataca tctaatagga gatgtgataa aatgggtgtt ttaacagaaa
    21781 acgaagtaaa aagaaaaatt ggaaaaaaca aaaataatat aactgaattt tatatagaaa
    21841 aaggtcaaat aataactcct tctgcaaaat cttatttagc tgagagaagt attgagctta
    21901 aatatgttga taaactttca gatgtaaaca atgaacctaa agaagttgta aaagaagtag
    21961 ttaaagagaa aaaatataaa tatactactg tatttggcgc taaatttgat gaaaaaccag
    22021 aacatatgac tcaattaaat ggaaatcttt tagtttttaa agaccataaa agaataatat
    22081 tgagaggaaa aatagattct cttgaatcag aaatactaaa ggtacagata ctttgtcaga
    22141 aactagatat gccaaagctt gttaaggaat tgcaagaagt attagacttt gtaagaaata
    22201 taatgagatg tgaagtttta gatgaaaagc tagatgactt taaactttta ggtttaactc
    22261 ctcaagaact tagagaacaa tctcactatc ctaaaaaata ttttggtata ggacatgagt
    22321 tcccaagtta tgaaatggga gaagtagtag tagctataaa tagtataaga agtaatacac
    22381 gagaggttga acttgcagcc tatgaagcat ttaagggtga gtatggaaat gttcatagag
    22441 aagatttaat aaaggcactt aatagattat cgtcagtatt ttggataatg atatataagg
    22501 ttagaactaa taagtataag tagttggagg acataatgga gaatctatta aatgaaatag
    22561 tggaagaagt aataactaga gttaaaaaag aagcatttat agaagttgaa gcatcaggaa
    22621 gacacgttca cttaagtaga gaagatgtag ataaattatt tggagaagga tatactctta
    22681 caaaactaaa agacttatct caaccaggtc aatatgcatg taaggaaaga gttacaataa
    22741 ctggtcctaa aggaagtata aaaaatgtag ttgtacttgg accatgcaga aatgaaactc
    22801 aggtagaaat atctttaaca gatggttcaa ctctaggact aaaagctcct ataaaacaaa
    22861 gtggagactt agaaggtact ttaagtatta aaatatcaac acaatatggt gaagttgaac
    22921 ttgataaggg attaatggtt gctaaaagac atatacacat gactcctaaa gatgctgaaa
    22981 aattcaatgt atgtgataaa gagatagttg aagctaaggt gatgagtcaa agacctttaa
    23041 catttgatga tgtagtaata agagtgagtg acagttttaa aacttatatg catatagact
    23101 atgatgaagc caatgcatgt ggttattcaa aaggaactgt tgctaaaata ataaagaaag
    23161 tataatttaa cctactatat agattacctc tagggaggat ttaatatgaa ttatgataac
    23221 cttgtagatt taataatgga agaaatctat aaaaaaataa atagtaatga aataaaaata
    23281 tctaataaac ctaaagctgt gattgtattt gaacaagaca acaataaatt taatttatta
    23341 aaagatgaat ttgaaatagt aaaatttgat aaaagtataa aggaatgtga aatagtgata
    23401 gtttcaagac tttgtatgag ggggctatcg aatatagcac ttggaaattc aacaagtgat
    23461 gaagaaagat ttatactaaa aatgattatg aagggcaaaa aagtatatgt cctagatgaa
    23521 ggaattgaat ataaaaaata taaggatact gcaccaaaag cattatataa gaaatttatg
    23581 tcatttgaag atgaaatatg taaatttggt gtagaaatta taaaagactt aaatattata
    23641 actaaaaata agttaaacat aaagaaagag ttagaaagta ctagaggttc taaaaaagaa
    23701 gatacaaaag ttttagataa agatgtttta aatttaacca gtgtagaaag tgtaaaaata
    23761 gataatgaat ttagtttaga tttaagaact aaaaaactta tatcggaagc tgacttaaga
    23821 aaaccaacta taaatggagt taaaaacata ttagttaata aaaaaagtat aataacacca
    23881 ttagctgtag attttatgag aatacaccat ctcaaactaa agaaagtgta aggaggtaat
    23941 tttatggata taggaaaagt tgtaggtaat gtttgggcca caagaaaaga tgaaaaatta
    24001 tgtggccaaa aacttttagt tgtaaaaatt ttaaaaacta aagacacata caaggaaggt
    24061 ttcatggttg cagccgataa tgcaggagca ggaaatggag acttagttct tatatccaaa
    24121 ggttcctcag ctagagagtc gattgaaaaa gctcatgttc cagtagatgc aactatagtt
    24181 ggagtagtcg attcgtttga ggtgtttgat gaataaaagt ataggagcta tagagtttaa
    24241 aagcattgct aaaggtatag aagtatccaa tgaaatgata aagaaatctt cagttgatgt
    24301 attgtactta aaaagtattt gcccaggtaa atttctaata atagtaggtg gagaaacttc
    24361 ttatataaat gaatgtgtag attatggaat aaaacttgga gaaggatata ttgtagataa
    24421 ctttgtaata aatgcaactt cacaagaaat attagatgga tttaaaaata agtatcaaaa
    24481 gttagatagt atagtctcta taggagttgt tgaaagtagt aaagtatgcg ctggaataaa
    24541 aatgcttgat aagactttaa aatccggcga cttagtatta gtaaaacttc aattatcttt
    24601 tgctataggt ggaaaattag tatatatagt agcaggagat ttaagtagtt tagaatatgc
    24661 attaaaagaa agtgaaaatg tagtaagaga aaaagaagta atatataaga ctgtaatacc
    24721 atcagtagac agtcaaataa taaaaagtct aataaaataa tggggggaat tatgagtata
    24781 aacgaaatta taatctatgt aatggtagtc ttcatggtgc ttggagctat agataaatgt
    24841 ataggtaaca aatttggtct tggagaacaa tttgaagaag gtataatggc aatggggtca
    24901 cttgcagttg caatggttgg tgttatatgt ttagcaccag ttcttgctga tgtattaaga
    24961 ccaattgtag taccagtatt taatatactt ggagctgacc cagctatgtt tgctggaagt
    25021 ttactagcta atgatatggg tggtgctcca cttgcacttg aacttgcgaa agaccctaat
    25081 gctgggttat ttggtggatt aatagtaggg gctatgatgg gacctacaat agtatttata
    25141 ataccagttg cgcttggaat aattgaaaaa gacgaccaaa aatttttagc aacaggaata
    25201 cttgcaggta taataacaat acctattgga gcattcgttg gtggattagt tgcaggattc
    25261 gagttaatga tggttcttag aaacttagtg ccaataataa tatttgcagt agttatagca
    25321 cttggattat taaaattaga aaatgctatg ataaagggat tcacatactt tggaaaaggt
    25381 gtagtaatag ttataacttt aggacttgct gctgccatag ttgaagcttt aacaggaata
    25441 gttgtaatac ctggaatgaa accaataggt gaaggtatag aaatagttgg agatatagca
    25501 atagttttag caggagcatt tccattagtg tttgttataa caaaggtatt taataaacca
    25561 ctaatggctt taggaaaagt tcttggaatg aatgatgtat cagcagcagg tcttgttgca
    25621 agtttagcta actgtatacc aatgtttggt atgatgaaag atatggacaa tagaggaaag
    25681 ataataaacg tagcattctc agtatcagca gcattcgtat ttggagacca cttaggattt
    25741 acagcaggat ttaatgctaa tatgataaca ccaatgatag ttgctaaatt agttggtggt
    25801 ataacagcag tattgttagc tatggttatc gcaaataaga cactaaaaaa ggagaatgta
    25861 taagatggat atatcaaata tagataaaaa tttaattgaa acattagtaa gacaaataat
    25921 agaagaaaaa ataagtgaaa ctaaagacac agtagatttt gttagaaata aagacattag
    25981 tggaatcact tctataaagt taccaactgt aaaagttagt gaatctgata gattagatac
    26041 aggaaatcca agtgatgtag tttacacaaa agatttattt acattagagg aaagcccaag
    26101 attaggttgt ggtatgatgg aaatgaaaga aacaactttt gattggactc taaactatga
    26161 cgaaatagat tatgtaatag atggaacttt agatataata atagatggaa gaaaggtttc
    26221 tgcttcttca ggtgagttaa tattcatacc aaaagggagt aagatacaat tttctgtacc
    26281 tgattatgct agatttatat atgtaacata tccagcagat tgggcatctc aaaattaata
    26341 aaaatcaagt taagttaata ataatcaaat aaataattta aaatacaaac tcttaaaata
    26401 caaactctta aataaacaca catatttaca aaagctatga attaaaattc atagcttttt
    26461 ttataaagaa cattatattt tagacgtatt ttttctatat ttactgggag acatattata
    26521 ttttttatta aataatctaa taaaatgctc tacatttgga taaccaacat tgttgctaat
    26581 atcagcaata gaaatatttg tattaattaa caaactttca gctctttcaa gtctaattct
    26641 ttgtaataat tgagtaaaat tacaaccagc aatttcttta ataatcttag aacaataagg
    26701 tatagaaaag tgaaattcat tagcaagttc ttctaaagtt atatcaaaat aattatcttg
    26761 gatataagta agaatatgtg tgttttttag attatctttc tttgtcatat taggaacttc
    26821 tatactattc tcatagcctc taagtaactt tgcaaaaaac accattatta aatgattaag
    26881 tatattattg taatatctat cttgtttact atattctgaa aacatctcta aaatagacat
    26941 ttctattgat ttatcattat ttgtatgaaa aagaatatag tcgttatgct tgtttttata
    27001 aatactattt aaaaagaagg atgaaagtaa attgttattt cttaaaaaat caaagaaagc
    27061 atcttcaaaa gtacttctac gtattaatac attaataata atactatcat caaatacccc
    27121 tatagaatgt tttactccag gtgaaactat acaaatatct ccttctttca tatgtatact
    27181 atggtggcta attatctggt ggcacttacc tgataaaaca taaatcattt caaagaatat
    27241 atgctcgtgc ataaattgag gtgtgtagcg attatgcttt ataagtataa cgttatcctt
    27301 actgctacac tcaaaataag tttcatcctt cataaattga ggaatctttt cattataaat
    27361 atatggaata attagattgt tattaactat gtaatcttca tttaaattat gaataaacaa
    27421 ctttagagat tctggatttt gctttgcaaa ataataattt ttataaaata actcagattc
    27481 attataaata taaagacttt gaaataattt ggtcaaactc ataaaatctc cttcagcttt
    27541 agaattgtta aatataaaat aaaaatatca taaatatgaa aataaatcaa ttaaattggt
    27601 taaaaacgac aatagtgtaa gtactttcta aaagatataa tgatgttagt cattagaaat
    27661 gataaaaaca acaaaataag gaggattata aaatgactac tttagaagtt gttaatgaaa
    27721 agaataaact aggagtttgg cgtcaaagaa taggttatgg ctcgtctgac ttagcttgca
    27781 acctaatttg gcaaatgata tcgttatatc ttttatattt ttacacagat gttatgggat
    27841 taagtgcagt agcaataagt tttatgtttg ttgtcacaag ggtaattgat ggagttacag
    27901 atttattggt tggttattgt atagataaga caaatactcg ttggggtaaa tcaagaccgt
    27961 atttcctatt tggtgcagta ccatttgcat tatttgcagt actggcattt agcgttccaa
    28021 aaatatcatc aaatggaaag ttaatttatg cttatgttac atatattggc ttgtctttaa
    28081 cttatactat agtaaatatt ccaatggctt caatattacc aagtttaaca gatgacacta
    28141 atgagagaac aacattgtca acatcaagaa aattctttgg attcttaggt tcaacaatag
    28201 taagttatag tgctttaact ttagtaggaa aatttggggg cactgatgaa gctctaggat
    28261 ttagaatagt tatgatgcta tttgcattta taggatgtgc aatattctta tttactttct
    28321 caaatgtaag agaacgtgtt gaggtaaaat ctgaaaatgt aaccttgtct caaacgataa
    28381 aatcattaaa agaaaataaa ccttggaaat tatttgcttt aaatatatta tttatgtgga
    28441 ctggattctt tattcaatct agtgcattaa tatattactt ctcatacaat attggaagca
    28501 aaacattggc aacaacagtg gctacaatta tgtcaataat tccaatactt gcaaatttct
    28561 tagtaccaat tttagctaaa agattaggaa aacgtaatct atacataact tcagcaacag
    28621 tacaatttat aggtctagga ataatactaa tagctgattt aaatacaagt ttaattatag
    28681 caggttctat agtttctgct tttggttatg gaataaaaga aagcatatat ttttcaatgc
    28741 aggctgaccc tgttgattat ggaatttgga aaactgggat taatacaact gggacattaa
    28801 actcaataaa tggattttta ggaaaatgcg cccaagctat ttcaggtggg ctaggtggag
    28861 cacttctagc ttggggtgga tacatagcta aagcagaagt tcaaccagta agtgcattag
    28921 tagctattaa agttatgtat gtttatattc caatgatact tttaatatgc tcaatgataa
    28981 ctatgaaatt ttatgattta gataagcaat atcctaaaat aaaagcagat ttagatgcaa
    29041 gaaataaatg aaaaataaat aataatgata taatcaagga gaaaaaacat gtctagtatt
    29101 acaaatagta taaaagactt caaaatggag attaatcaag acttcataaa taaagcagaa
    29161 agactaaaac cagctcttat agaaaaatta gttaaaccta agaaaattat taatattaaa
    29221 atgggtaaaa atgattggga agttatagat acaaaatcaa tagatgaaat atctaatata
    29281 agtttgggta agggtgatag tatttgtatt gactttggta atcaccaagt gggatatgta
    29341 agttttaaaa taaagccaat aggtagccca ccagatgcac ctgctcattt aaaaattaaa
    29401 ttaggagaaa cattatgtga aataggagaa gatagttcaa cttacaatgg aggtataagt
    29461 agtagttgga ttcaagaaga gactacgcat atagatattt tacctgatga aatcaatatg
    29521 aaaagaagat atgcatttag atatttagaa ataaaggtat tagatacatc acctaaatat
    29581 aaggtcatag tagaaaatgt atattgtaat tttgtatcat cagtagatat gtcagaggta
    29641 gactatctat caaatattga tgatgactta atccaaatag ataaaatagc tttgaaaact
    29701 atgcaagact gtatgcagac tgtttttgaa gatggaccaa agcgtgatag acgcctttgg
    29761 gtaggagatt taagactaca agctataaca aattatgaaa cttttaaaaa caatgattta
    29821 gtaaaaagat gtttatatct tttcgcagga ttaactcaaa ataaaggtca aataggagca
    29881 tgtttattta tagaacctaa attattagta gatgatactt ctttatttga ctattcatta
    29941 ttcttcatat catgcttaca tgattattat gaagcaacta aggatatgga agttttaagg
    30001 gaattatggg cagttgcata ccttcaagct aagttagctt tagaaagagt tggaactaat
    30061 gatgtggttt atgatagtga tgattggtgg tgtttcttag attggaatga gggtctaaat
    30121 aaacaagcag gtgcacaagg agtattaata tatactctaa aacaagctca caggttagct
    30181 atagtattgg gtgatagcga aaaaaaagaa gaacttgagt atgaaataaa tagagtggtt
    30241 caaggtgcta aaaatcattt atgggatgaa aaacaaggat tctttgtaag tggtagtgaa
    30301 aagcaagttt cttggatatc ccaaatatgg tgtattcttg ctgatgtatt tgataaggaa
    30361 acaaatacaa agatattaga taacctatta gataatccac ctgctataga tatagttact
    30421 ccatatgcat atcatcacct agttgaatgt ttaatattga ataatagaag agaaaaagct
    30481 cttgaatgta taagaggtta ttggggtgaa atggttaagg atggagctga ttgtttctgg
    30541 gagctatata atccaaatga taagtttgta tctccttatg gttcaaggat aataaacagc
    30601 tattgccatg cttggagttg tacaccatct tattttataa gaaaatattt tgtatagatt
    30661 taattttccc tatcactaat cttacatttt aaaatatcat ccttattcgt aacattatca
    30721 taagaatttg tttcttatct taaactaatt aaaattataa agtttattac catgttatta
    30781 ataaaataaa tttttacaat ggttttcttt atcgattgaa ataaaaggta atatgatata
    30841 atatctaagg agagaatctt atcaattaga atatgcatag aaacaagtgg cttaaaagga
    30901 gacgttatat ggaagagata gtaaagaagg aacaagcaag tatgaatgat atttttttgt
    30961 atcttaaagc gaagtcaaat tcattatata agtttgtaat attatataat gagtttatca
    31021 ataaacctaa agattatggg actggggaac ttataaacat ggttgaggta catatgctaa
    31081 cagcgattga agaaaatcca ggaataagtc caactcagtt agctaaaata tggaacagaa
    31141 ctaaaggtgc aatatctcaa actatatcta aattggagaa aaaaggttat ataactaaag
    31201 aaaagaaaga aggaaatgca aaaactatat tactatatcc aaccacatca ggagtagagt
    31261 taagtaaggc acataaacta tatgacacca tagatgttac aagaacattt gaatacttaa
    31321 tcaaggagtg tacattagag gagatagata gtttttataa agtaattgat ttgtttatta
    31381 atttattaga agaatctcca caggaaaata atgattctga aaaagtatag ctaagattga
    31441 agctaagtag aaagaaatag atgatatata gttagtatat agaggtagtg attaactaca
    31501 tat
//
LOCUS       DAEPXF010000025        31195 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain HB27
            SAMN13376389-rid12479423.denovo.026, whole genome shotgun sequence.
ACCESSION   DAEPXF010000025 DAEPXF010000000
VERSION     DAEPXF010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13376389
            Sequence Read Archive: SRR10518432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 31195)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31195)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001069624.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001069624.1
            Genome Coverage       :: 315x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/15/2021 01:54:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,165
            CDSs (total)                      :: 4,076
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 89
            rRNAs                             :: 10, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 10 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 19 of 69
            Pseudo Genes (incomplete)         :: 44 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 10 of 69
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31195
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13376389-rid12479423.denovo.026"
                     /strain="HB27"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="China: Hebei"
                     /collection_date="2017"
     gene            complement(<1..841)
                     /locus_tag="KRL67_001057"
     CDS             complement(<1..841)
                     /locus_tag="KRL67_001057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein PilM"
                     /protein_id="HBH1521956.1"
                     /translation="MAIIKKILNLEINDDYIRLVFLRKSHKKVFIDKSIIKEINFDIK
                     NYSNSIEKIKYIEDVVRFVKEEIGKSKILYKNLYFNIQIQDIVIRNVEVLNTKKKRDI
                     LSMIEFEITQYIPININNYSIKYKVINSKNDKLNIQVILFPKSIIQLIKNISENLNMN
                     PRTININFDILQKLINLNLIENFSEEGVFIECKKSQLIINITKDKKIRETYLLSKEGK
                     SYEFIKRLLRDFKYVYYYGIENCFIEEYFKYTDKSIEVNPLRLKDKVKVENNFDTDLE
                     DNRI"
     gene            complement(895..1245)
                     /locus_tag="KRL67_001058"
     CDS             complement(895..1245)
                     /locus_tag="KRL67_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HBH1521957.1"
                     /translation="MINKKRKGFTLVEMIVVVTILGVISSIALVKYSKVQESAKLNAD
                     YTNAANIVTAASMAINDDEKIIDSLSVETLKEKGYLNTVPVPQSTSGKFELVINDNGT
                     DISVNINSKQFYPK"
     gene            complement(1299..2384)
                     /locus_tag="KRL67_001059"
     CDS             complement(1299..2384)
                     /locus_tag="KRL67_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HBH1521958.1"
                     /translation="MRLSKNIFYNLEKFYLVLNRISDKEIRVICKEIGILLESGCEIT
                     KIFEIIESQSSKKVKNLLSIVSNHIQKGNSIAESFQITGTFSKFFISMIKAGETSGNL
                     DIIMSDLSNYYDKEYKLKMKIITISIYPIILIILSILSMLFIFVFVIPNFQVVYTSNG
                     IEPPLITRALIGISTIVTNNLAYLIFSFILFSIGSIYFFITNDNIKKLINSLKFKIPF
                     IKRINQLVATTRFSRALSVLISSGIQIVEAIDMSASVIDNEFIYERLKISNNYIKKGN
                     SIGKSLNLANIFPKLFISMANTGEESGKLDKSLNVINKFYENELNNKIEQIMKFIEPM
                     LIVFMGLIIGTFILAMVVPMFDAITSF"
     gene            complement(2555..3934)
                     /locus_tag="KRL67_001060"
     CDS             complement(2555..3934)
                     /locus_tag="KRL67_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II/IV secretion system protein"
                     /protein_id="HBH1521959.1"
                     /translation="MIKIILEESSLPMLIPEKLAKKYNVFPVEFQDDNLVVEIEDEDI
                     YALQDLRLATGKEIILKKEKKELISEKIEKYYNSFELDEDYAKKLFENLLEKAIKENA
                     SDIHIEPFKEHLIVRMRVDGELKEVSKFLVDVYPSLSTVIKLKASMDITEKRLPQDGR
                     VDIKLGGNLIDIRVSSIPTVYGEKIVLRILNRDTFLKDKLELGFSDEAIKSINRIINK
                     RAGILLVTGPTGSGKTTTVYSLLNDLRGINKNIMTIENPVEYKMDGISQIQVNSKVGL
                     SFDVGLRAILRQDPDIIMVGEIRDAETAKIAVRAAITGHLVISTLHTNDAVSSIARLL
                     EMQIPPYLLNASLIGVISQKLVRKVCNHCSHEIMIKDNFSGDVNTKVAVGCEKCNDKG
                     YFGRTAIYEILEINDDIKTCIRNMEDSSTIKDVAQNNGMITFEDSCKRLINEKITTLE
                     ECLVVNEMI"
     gene            complement(3927..4607)
                     /locus_tag="KRL67_001061"
     CDS             complement(3927..4607)
                     /locus_tag="KRL67_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF881 domain-containing protein"
                     /protein_id="HBH1521960.1"
                     /translation="MKKQYVFIILASTILGILVGTIFRDYSNEETVYTSKDKSIRKEI
                     KMTRKSIKELTKEKDKLDKEINSMKQNQKNSEEISEIETLRDSLSYSDVKGSGIILSI
                     DAIDEKTGNIANSIDYNKILVKVINELKVNGGKYIEINGQRINQYSEVVLAGSHININ
                     GVPVAQPYEIKCIGDKEKLSNYVDKGSKYLDSIQTNYPMKISIKTEENISMKKMNIPN
                     KLKYIKGD"
     gene            complement(4999..6078)
                     /locus_tag="KRL67_001062"
     CDS             complement(4999..6078)
                     /locus_tag="KRL67_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBH1521961.1"
                     /translation="MEGKVKKAFPGGNTSQGSYSFFDYIIPENVKRVFYLKGGPGVGK
                     SSLMKKIYKEFLHRGYDIDLYHCPSDPSSLDGLVIKKLGVVLMDATAPHTMDPKLPGA
                     LDEIINLGDYWNTEELEKNKQEISVYDKDISNSFKRAFKFLKSAEPIFRDIEEKYSDC
                     MDYGKVNLVTEEFIKRLFDGVKSTGNLKREKHLFGSAITPVGCLDYTENILKDVNKVY
                     YLAGEIGTGKTTLLNKVYKKAVEKGLNVEVYHYPLIPEKIETVLLTDLDIGITISTTF
                     KEEDTIDLNQFLNREKLLKYEEDIKFDEKVLDDLICWAVLNLKRAKSKHDIIESYYSP
                     NMRFDEVGKLRDKLIKRILKYEENL"
     regulatory      6374..6489
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            6664..8034
                     /locus_tag="KRL67_001063"
     CDS             6664..8034
                     /locus_tag="KRL67_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBH1521962.1"
                     /translation="MKQKWIVLIIICIGVFMSTLDGSILNIANPTIAADFKINMSQIQ
                     WVVTAYMLVVTATMLFFGKLGDKVGSNRLYTLGFFIFTIGSFLCSMSNNLSTLISSRI
                     FQAVGASILMATGLGIVSNAFPANEKGKAIGITGAVVGIGNMSGPVIGGIILEHFGWP
                     SIFIINIPIGIIAVFLGIKFLPKPVLDEQNKSFDIPGLLLFASCTTLILLAMNENGNT
                     RLYLGITALIIFLLLALREVKFEQSFIDLPLFKNRNFTVGNVIGVACYFPQMAVSFLL
                     PFYLEQLKNLSPMMAGYVMTVHPLIMVLIAPIAGSLSDKHGAKNILTASFSFMTISLV
                     GMALLKADSPLYLLIVCLVIFGLGLGAFSSPNNSSILADVPPQKQGYGGSFLATIRNL
                     SFALGTAFFSSFFAQSLAYNQKFKSHTSAYVIASNQSYWIAASVCFIGLILTVFFMRK
                     TDKSIS"
     gene            complement(8299..8910)
                     /locus_tag="KRL67_001064"
     CDS             complement(8299..8910)
                     /locus_tag="KRL67_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihA family ribosome biogenesis GTP-binding
                     protein"
                     /protein_id="HBH1521963.1"
                     /translation="MKIRSSEITMSAVNKSQYPAEGIPEIALAGRSNVGKSSIINTLL
                     NRRNFARTSQTPGKTRTINFYLINNEFYFVDLPGYGYAKIAKSEKEKWGGIMERYLES
                     RQELCSIFLLVDIRHEPTADDKLMYEWIKHFGYNCVVIATKADKISRGQYQKHISIIR
                     KKLQMESSEKVIPVSSLKKTGVEELWEEIVNQYNQHGYEITVD"
     gene            complement(8900..11263)
                     /gene="lon"
                     /locus_tag="KRL67_001065"
     CDS             complement(8900..11263)
                     /gene="lon"
                     /locus_tag="KRL67_001065"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HBH1521964.1"
                     /translation="MEQNYTKIDHELPLIPLRGLAIFPYMILNFDIGREISLKALDQA
                     MMDEELIFLTSQKEAEVDEPGEEDFYHVGTICKVKQMIKLPGDTVRVLVEGVSRGRVK
                     KIEQEDGYFRAVIEEIVFDSDNLDSETEVEIEAFVRNVFDAFEEYINIGNRVSPEILI
                     SLADIEDVDRFIDTIAANIYLKSSQKQEILEEFDIRKRLELIYSILLEEIDILKIEKK
                     ITLRVKKQMNKVQKEYYLREQLKAIQKELGEEEDINSEADEYREKLKKIKAPKTTKEK
                     IEKEIDKFSKISSMSPDVSVSRNYLDTIFSLPWNKETKDKLDITKAKDILDEDHYGLE
                     KVKERILEYLAIRTLAKSLKGPIICLVGPPGTGKTSIVKSIARALNRKFVRISLGGVR
                     DEAEIRGHRRTYVGSIPGRIINGVKEAQTKNPVFLFDEIDKMAADYKGDPASAMLEVL
                     DPEQNKDFVDHYLEIPFDLSKILFVTTANSLGNIPRPLLDRMEVIEVSGYIEEEKLNI
                     AKKYLLPKQIKEHALKENFIKIDDETLRSIINHYTREAGVRTLERTIGKICRKVAKKY
                     VEDPTLEEVVINKSDLEAYLGKDMFKYQLAEVNPQIGLVNGLAWTEVGGVTLEVEVNV
                     LKGKGEIVLTGKLGDVMKESAKTGISYIRSIVDKFDIDPDFYKTNDIHIHIPEGAVPK
                     DGPSAGITMALAVISALTKRPVPGNIAMTGEITLRGRVLAVGGVKEKLLAAHRAGITK
                     VLIPKECEADLDEIPENVKEKMEFVLVEHMDEVLEQALLKSGENNEN"
     gene            11443..11955
                     /locus_tag="KRL67_001066"
     CDS             11443..11955
                     /locus_tag="KRL67_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HBH1521965.1"
                     /translation="MLKLFLTFLKIGAFTFGGGYAMVPLIQDELVNKQKLLEQEEFID
                     YLSIAQSYPGVLAVNISVLLGYKLYGIPGVLICTLGSALPSFCIVLVLSYLYFKNSSS
                     KILDGFFKGVAPVVIALILYSFTSMFKKLPKSKTNILLLIIAVLSVGVFNISPIYLII
                     GGGVYSLCQK"
     gene            11943..12470
                     /locus_tag="KRL67_001067"
     CDS             11943..12470
                     /locus_tag="KRL67_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HBH1521966.1"
                     /translation="MSEIILLFLIFLKIGAFSFGGGYAMLPFIQQEFISKYHFITNQE
                     FLDLLAISQSTPGPVAINSATFVGFKTAGFFGSLAATIGVTIFALIGLTIISKLFSKF
                     KNNKKVENLLTVLRPITLGFILSAAFSSAKEVNWDLYGIIAFIASFYLLYKGKIGSIA
                     IVFVFGILGILLTFL"
     gene            complement(12541..13791)
                     /gene="clpX"
                     /locus_tag="KRL67_001068"
     CDS             complement(12541..13791)
                     /gene="clpX"
                     /locus_tag="KRL67_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HBH1521967.1"
                     /translation="MSKYEEKRQLKCSFCGKNQDQVRRLIAGPNVYICDECVELCDEI
                     IQEEIEDTIDEDTTSLPKPKEMMEILNDYVIGQEKAKKALSVAVYNHYKRIYSKKSSS
                     KDIEIQKSNILLLGPTGSGKTLLAQTLARTLNVPFAMADATSLTEAGYVGEDVENILL
                     KLIQAADFDIEKAERGIIYIDEIDKIARKSENPSITRDVSGEGVQQALLKILEGTVAN
                     VPPQGGRKHPHQEFLKIDTTNVLFILGGAFDGLEKIIQKRGGDKTLGFGAKIESKKEL
                     DLGKLYEKVLPEDLLKYGIIPEFIGRIPVLATLELLDEDALMQILQEPKNALVKQYKK
                     LLELDDVELEFEEGALRAIAKKAIERNTGARGLRSIVESVMMETMFEVPSRDNIKKVI
                     VTEKSVNEDSVNPIIVLKDQEESA"
     gene            complement(13810..14394)
                     /gene="clpP"
                     /locus_tag="KRL67_001069"
     CDS             complement(13810..14394)
                     /gene="clpP"
                     /locus_tag="KRL67_001069"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HBH1521968.1"
                     /translation="MALVPVVVEQTGRGERSYDIFSRLLKDRIIFLGDQVNDATAGLI
                     VAQLLFLEAEDPDKDIHLYINSPGGSITSGMAIYDTMQYIKPDVSTICIGMAASMGAF
                     LLAAGAKGKRLALPNSEIMIHQPLGGAQGQATDIEIHAKRILKIKETLNEILSERTGQ
                     PLEKIKMDTERDNFMSALEAKEYGLIDEVFTKRP"
     gene            complement(14544..15830)
                     /locus_tag="KRL67_001070"
     CDS             complement(14544..15830)
                     /locus_tag="KRL67_001070"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HBH1521969.1"
                     /translation="MKAELIKKEGNKVTLKITVDNNKFEAAVNKAYNKSRNKYNIPGF
                     RKGKAPRIVIETQYGKGIFYNDAIEILFPEVYPEAIKELDIDPIDNPDLDIEEISKDN
                     GLVMVLNVEVKPEFELGNYKGIEIAKVENTVSDENVDAKLQEMVEKNARLVSVEDKAL
                     EDGDTAIIDFEGFENGVAFDGGKGENYNLVIGSNTFIPGFEEQLVGKKAGEEVEVNVT
                     FPEEYHSQDLAGKPVVFNVKINDVKVKELSALDDEFAKDTSEFDSLDELKADVRAKLE
                     EEAKNRADAETRNSVVEKVAENTEIEIPEVMIQHQIDNMLNELNYQLQYQGFGLQQLL
                     EMTGRTMEELREERKEDAKKLVKSSLVLEAITKAEGIEATEEEFKAELEKMASAYNME
                     VEKIEASLRDADKEDIKGQIKIRKTIDLLVDNATIA"
     gene            complement(16024..16494)
                     /locus_tag="KRL67_001071"
     CDS             complement(16024..16494)
                     /locus_tag="KRL67_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HBH1521970.1"
                     /translation="MKIGIVSDTHMMIKNMEKTIPYLKECDLIIHAGDNFTDSRYIHS
                     MTNVGIIAVKGNCDFDAVEEEVVFEVANKTIFLCHGDKYGVKYGTHMLEKKATEIDAD
                     IVIFGHTHTPFREVKDGVLYINPGSTSLPRGVSYKSFVIMDIEEDNINIEEIRI"
     gene            complement(16494..17843)
                     /gene="rph"
                     /locus_tag="KRL67_001072"
     CDS             complement(16494..17843)
                     /gene="rph"
                     /locus_tag="KRL67_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HBH1521971.1"
                     /translation="MTRSYDRSNEQIRPVKITRDYTKYAEGSVLIEMGETKVICTASV
                     EEKVPPFLRNSGTGWINAEYSMLPRATQQRKIRDASRGKIDGRTQEIQRLIGRAIRSV
                     VDLNKLGERTIWVDCDVIQADGGTRTASITGAFIAVAEAIYKLYKAKTIKEMPITNFV
                     SAISVGIVNGIHMLDLCYEEDSKAQVDMNIIMTDKCEFVEVQGTGEEKPFSRADLNLL
                     LELGEKGNKELIRLQREALGEIADEILGMDYGNEVVIATNNAHKLEEIGEILKDFEYK
                     VYSLKDVDLAGIEIVEDGKTFEHNALIKARTIAKKTKLIAISDDSGLEVDALGKKPGV
                     YSARYAGEHATDEENRKKLLKAMQNVPMSKRNARFVSAIAVVFPDGKEFVVRGICEGM
                     IGFEEKGKNGFGYDSLFIVKGYDKTFGEIPSVIKNSISHRANALKLMKQEFIKRVVK"
     gene            complement(18003..20027)
                     /gene="ligA"
                     /locus_tag="KRL67_001073"
     CDS             complement(18003..20027)
                     /gene="ligA"
                     /locus_tag="KRL67_001073"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HBH1521972.1"
                     /translation="MSVKDEINKLIETINYHNERYYNQDSPEISDYEYDKLMKELISL
                     EEEHPELKRIDSPTNRVGGKPLDKFNQIVHKTPMLSLSNAFSEQDLRDFDKRVQEYVG
                     ENVEYVVEFKIDGLSVGLTYNNGEFEKGATRGDGIVGEDISQNLMTVKSIPLKINDKK
                     ELIVRGEVYISKDNFRKVNEQQEEQEQPLFANPRNLAAGSLRQLDPKLTAKRPLDIFV
                     FNMENIEEYDLKSHSESLEYLEKLGFSVSQSYKVCKSIDEVIEHIEYWTDNRGNLDYE
                     IDGMVIKVNNIKQRDEMGYTAKSPRWAIAYKFPAEKKKTKILDIIVEVGRTGTITPSA
                     ILEPVRLAGTTVSRATLHNEDYIREKDIKINDTVLVQKAGDIIPQVLEVIKEERTGDE
                     IDFKMPEKCPVCSEPTIRLEGEAAVKCINMSCPAQIRRGIIHFVSRDAMNIDGLGESI
                     ITLLLNEKIIQDVSDLYYIKKEDVVDLERMGEKSADNLINSIEKSKENDLWRLINGLG
                     IKFIGVKAAKILAYNFKDLDEVISATSEQLEELEEFGSIMANSVVKFFKEDKNMNVIN
                     KLKNVGVNTKSLDNTGEKVEKIFEGMKIVLTGTLPTLKRNDAKDMIEKRGGKATSSVS
                     KSTTFVLAGEEAGSKLTKANDLGIKVIDEERFLELLKLSSKDEVKAVLES"
     gene            complement(20213..21151)
                     /locus_tag="KRL67_001074"
     CDS             complement(20213..21151)
                     /locus_tag="KRL67_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HBH1521973.1"
                     /translation="MKVLFTINYGKEKFDKIRELGYDVIHYSEDSIENNEDVNTADVL
                     VTYNPFERLDISKMKNLQYIQTTSVGIDQLPKDEVLKRNIKIANNRGGYSVPIGESIV
                     LYILEIYKNSAKFFKQQQNKEWKMDFSVSELYGQRVGFLGTGTISSEAAKRLKAFGLE
                     IWGVNTDGSSKKYFDKCFATEDMNIVFEECDIVVVAMPSTKSTDGIVNKDMFKLMKDG
                     SVFINVGRGNTVNQKDLEECVEKFRGVALDVFESEPLNKDNKLWECENVIVTPHNSWV
                     SDKNKERTFNMVYNNLKHYIMENKPVDNVVDISRGY"
     gene            complement(21253..21675)
                     /locus_tag="KRL67_001075"
     CDS             complement(21253..21675)
                     /locus_tag="KRL67_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3783 domain-containing protein"
                     /protein_id="HBH1521974.1"
                     /translation="MSFEKINNNNLDKIHNRKCIMIVNFNKKELSLIKNVSNFAGIRD
                     HIILSDKNGENIIRDILDGNISNECENGIKSKSVIFNNTPSTQVNAFLEGLKKVRINR
                     PFTAMVTETSIDWTLNNLIENLVQERNAMSLGKTIEHK"
     gene            21863..23146
                     /locus_tag="KRL67_001076"
     CDS             21863..23146
                     /locus_tag="KRL67_001076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugated bile salt MFS transporter"
                     /protein_id="HBH1521975.1"
                     /translation="MNTLLNNKFSIKNFSNAWVIVLGCMLIQAVPFSIVASIQPQFIS
                     YVVEGEGFSLASFSLIFTIGTIASAIASPFIGVLFSKVNIKILYLLGCIISSIAFATF
                     SICTELWQFYTVSCIVQIGATIISAIGIPVLISAWFDEESKGKALGFAFAGGSIGSIF
                     LQPLAVRLLATQGYSRSYLIFGIIALIVGIPVILFILRMPKDINEKVKSKGKKEKASD
                     TISPSVWGYTLKEAKKIKYFWIMTIGFIFVGIYVSSLSVQYPTYLRNYLNPILIGTVG
                     STFALFALIGNLFGGLLFDKFGQTKPLIISFVLVLIATLSLLLVDKSSAFAFIFAILK
                     GLSVFAYMIGPAYLTGSFFGQKEFGSIYGIVQLIFAVGFALGSTLFGLLVDNFGYRFS
                     WSIIILSVIIAYTLLIITSIGMKKLNKERLDKLMQ"
     gene            complement(23224..23739)
                     /locus_tag="KRL67_001077"
     CDS             complement(23224..23739)
                     /locus_tag="KRL67_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HBH1521976.1"
                     /translation="MNYFVIADPDKCIGCRTCMIGCVVAHSDEDIFYQNPDEINFNPK
                     LSVIKTKEVSAPIQCRHCEDAPCAKACPNGGIVRVGNTIKINEENCIGCKTCMLACPI
                     GAIDIVTLKDVDEGKLCFRERMTANKCDFCIESPEGPACVNVCPTKAFTIVKDEDIDS
                     KVKNKRKLAIL"
     gene            complement(23739..25115)
                     /locus_tag="KRL67_001078"
     CDS             complement(23739..25115)
                     /locus_tag="KRL67_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron hydrogenase small subunit"
                     /protein_id="HBH1521977.1"
                     /translation="MIINIDKDLCTGCRECSKVCPVNAIEGEEGKPQEINLDRCVMCG
                     QCVQTCKSYASVIDEGFEFLQEKKQEREIPESINEPIFAAYSVCDIDKVKKALSDPNV
                     FTMVQCAPAVRVALGEDFGYYLGYLGAGKMAAALRALNFDRVYDTNFAADLTIMEEGS
                     ELIKRVTEGGTLPMFTSCCPAWVKFMEKNYPKLTNHLSSCKSPQQMGGAIFKTYGAEI
                     NNVDASKIFSVSIMPCTCKKYECDREEMDSSGYRDVDVVLTTRELAYLIKDMGIDFKN
                     LREEKFDSPLGSYSGAGTIFGVTGGVMEAAIRTGYELITGESIPDVEVKQVRGENGFR
                     KSTIKIGDLDLRVGVVSGLKNVIPVLEDLEKGKLDVDFIEVMTCPVGCVSGGGQPKIL
                     LEEYKELAYENRTKATYVHDEHLAIRKSHENPDIKKLYDEYLVEPLGEKSHHLLHTTY
                     CIGKEVAK"
     gene            complement(25140..25691)
                     /locus_tag="KRL67_001079"
     CDS             complement(25140..25691)
                     /locus_tag="KRL67_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HBH1521978.1"
                     /translation="MKNKIGSFVIADPNKCTGCRACEVACFTMHNQHNNVGYTVGTVD
                     IPVIPRLYLVKGDNFCMPIQCRHCEDAPCLNSCPQKAIVKENNIMSINEEKCIGCKTC
                     LLACPFGAIDLLPQYEDGKEVEQVILDENKKIAYKCDLCKDNEKIACISACPQEALKL
                     VTPIDDKKAKNRQAALSLLLTNK"
     gene            complement(25728..25943)
                     /locus_tag="KRL67_001080"
     CDS             complement(25728..25943)
                     /locus_tag="KRL67_001080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH1521979.1"
                     /translation="MQIHRELSKSENKYMCTKKEEQKYSKCEICGVLIDDTDKVYNNT
                     YNLCKKCKRNISTEKIVKYINIYDESN"
     gene            complement(25912..26745)
                     /gene="fdhD"
                     /locus_tag="KRL67_001081"
     CDS             complement(25912..26745)
                     /gene="fdhD"
                     /locus_tag="KRL67_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HBH1521980.1"
                     /translation="MNNIYSKTKLQENSKDKVFENSYSLVDYKYLNSFNVEVSKINEH
                     EVSIDNEEIIAEYPLSFILNDKYVNTFLCTPYNLEELIVGFLKTKGYIENKKSLLKFE
                     INESERFAKVTIQEKQKNQDEQIIFLNSLDYITCASVESNIKINYDTIYSIMDRNLNS
                     SELFKNTGGVHSVSVFNFDKKIVTCEDVARHNAMDKAIGFCVLNDITLKDKIVAVSGR
                     ISLEMILKAAMTQIPIVVSKSAPTNLSIELSKKLNITLIGFVRGRRMNIYANPQRVIK
                     K"
     gene            complement(26794..28938)
                     /gene="fdhF"
                     /locus_tag="KRL67_001082"
     CDS             complement(26794..28938)
                     /gene="fdhF"
                     /locus_tag="KRL67_001082"
                     /EC_number="1.17.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:complement(28522..28524),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase subunit alpha"
                     /protein_id="HBH1521981.1"
                     /translation="MEKKILTVCPYCGAGCQLYLVVKNNKILRAEPANGRTNQESLCL
                     KGRYGWDFLNDPKILTPRLKKPMIRKQGVLEEVEWDEAINYTASRLNEIKEKYGPDSI
                     MGTGSARGPGNEANYIMQKFMRATIGTNNVDHCARVUHAPSVAGLAYSLGSGAMSNSI
                     PEIENADVLFIFGYNGADSHPIVANRIVKAKKNGAKLIVTDPRVTESARIADIHLPIK
                     GGTNMVLVNAFGNVLIEEGLYNKEFVQNHTQGFDEYKEIVKPYTAKYAEKITGIPEEL
                     IRKAMREYAKGKKAMILYGMGVCQFGQAVDVVKGLASIALLTGNFGRESVGIGPVRGQ
                     NNVQGACDMGALPNVYPGYQNVTDDKIREKFEKAWGVKLSPNNGYSLTQVPDLVLKEK
                     KLKAYYIFGEDPVQSDPDASEVREALDELEFVIVQDIFMNKTALHADVILPATSWGEH
                     EGVYTCADRGFQLMRKAIEPQGDVKPDWQIISEISTAMGYPMNYKNTKEIWDELRQLC
                     PSFLGATYEKIETQGCVQWPCKSESMEDKGTMYLYEGQKFSTPNGKGNLFAAEWRPPM
                     EVEDDEYPFSLCTVREVGHYSVRTMTGNCRTLSSLEDEPGRVQINSNDAEKLGIEDDE
                     LVRISSRRGSVITRATVTDRVKEGATYMTYQWWVGACNELTIANLDPISKTPEYKYCA
                     VKLEKLEDQELAEKCVREEYQSLKDKMTATNI"
     gene            complement(29189..30247)
                     /locus_tag="KRL67_001083"
     CDS             complement(29189..30247)
                     /locus_tag="KRL67_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase-like domain-containing protein"
                     /protein_id="HBH1521982.1"
                     /translation="MDRLKNIRKSLLQYTFLFLLIGITIYLVFKTLDIKMLSNVMVMV
                     DKKFLFIGALAIMCHIMLEGVVMKIIIESTHKVNIRFIGFKLAIMGFYYNLITPFASG
                     SQPVQIYVLKKCKMPLSKASAVVTNKSIIFQIVVTFYCTILVLMNFAMLEKQMKMIMP
                     LVFLGIAINSFVLIMIILVILNPKKIKYFMRITIKHLSKIKFLKFLDNKIESIESFID
                     DYSKAINFFAKNKKVLISTIIVTFIQLSAYFSVSFWIYKAFNLQGYSYIYILTLQAFL
                     YMAISPIPTPGNVGANELAFFTIFKSVFPQPLMGYAVFLYGGFMYYLILIGSGIFTVI
                     THYRMKNRIGRNIVLSEN"
     gene            complement(30458..31117)
                     /gene="cprR"
                     /locus_tag="KRL67_001084"
     CDS             complement(30458..31117)
                     /gene="cprR"
                     /locus_tag="KRL67_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator CprR"
                     /protein_id="HBH1521983.1"
                     /translation="MSKILVVDDELDMLKLIENVLKRDGHEVKVISDVEKAMNMDFNY
                     FNLIILDVMMPKMDGFEVCKTIRNKVDCPILFLTAKNMESDIMYGFGIGADDYITKPF
                     GVGELRARITAHLRRESREKKNLLTISNVQFNFLGKEVLIDGKKINFTKSEYLICEFL
                     AKNKGQVFSKERIYESVYGFDGESDISTITEHIKNIRSKLKQYDIYTIETVWGIGYKW
                     V"
BASE COUNT         9430 a         5611 c         3345 g        12809 t
ORIGIN      
        1 ttatcctatt gtcttctaaa tctgtatcaa aattattttc tacttttact ttatctttta
       61 atcttaatgg atttacttca atagatttat ctgtatattt aaagtattct tcaataaaac
      121 aattttctat accatagtaa taaacatact taaagtccct taataatctt tttatgaact
      181 catatgattt tccctctttt gatagcaagt atgtttctct tattttttta tcttttgtta
      241 tatttataat taactgactt tttttacatt ctataaatac tccttcttct gaaaaattct
      301 ctattaaatt taagtttata agcttttgta atatatcaaa atttatattt atagtccttg
      361 gattcatatt tagattttct gaaatgttct taattaattg aataatactc tttgggaaaa
      421 gaattacttg tatattcaat ttatcatttt tagaatttat aactttatat tttattgaat
      481 agttatttat gtttattgga atgtattgtg ttatttcaaa ttcaatcatg cttaatatat
      541 ctcttttttt cttagtattt agaacttcaa catttctaat gactatatct tgaatttgaa
      601 tgttaaagta taaattttta tatagtattt ttgatttacc aatctcctct tttacaaatc
      661 taactacatc ttctatatat ttaatcttct caatcgagtt actataattt tttatatcaa
      721 agttaatctc ttttataatt gatttatcta taaaaacttt tttatgactt tttcttaaaa
      781 atacaagcct tatataatca tcatttattt ctaaatttag aattttttta attatagcca
      841 tcaccttctt aaataaaaat aaattttaaa acttacaaca atcaacataa aatattattt
      901 tggataaaat tgcttagaat ttatatttac gcttatatct gttccgttat catttatgac
      961 aagttcaaat ttacctgatg tactctgagg aactggaaca gtgtttagat atcccttttc
     1021 cttcaatgtt tctacactta gagagtctat tatcttttca tcatcattaa ttgccatgct
     1081 agctgctgtt actatattag cggcattcgt atagtctgca tttaatttag cactttcttg
     1141 aaccttacta tacttaacta atgctatact agatataact cctaaaatag ttactactac
     1201 aatcatttca acaagtgtaa atccttttcg ttttttattt atcaattaaa tattctctcc
     1261 ttttcaaaag tttcatctaa taaaatttat ttttctgtct aaaaactagt aattgcatca
     1321 aacataggaa caaccattgc taaaataaaa gttccaataa ttaatcccat aaatacaatt
     1381 agcataggtt ctataaattt cattatttgc tctattttgt tatttaattc attttcataa
     1441 aatttattta ttacatttag acttttatct agcttcccac tttcttctcc tgtatttgcc
     1501 attgaaataa ataattttgg aaaaatattt gctaaattca aagatttacc tatgctattt
     1561 ccttttttta tataattatt agaaatcttt aacctttcat aaataaattc gttgtctata
     1621 acactagctg acatatctat agcttctaca atttgtattc cactgctaat taaaacagac
     1681 aatgcccttg aaaatcttgt tgtagctact aactgattaa ttctttttat aaaaggtatt
     1741 ttgaatttta acgaattaat caattttttt atattatcat ttgttataaa gaaataaatg
     1801 cttcctattg aaaacaatat aaagctaaat attagataag ctaagttatt tgtaactatt
     1861 gtagatattc ctattaaagc tctagttatt aaaggtggtt caattccatt gctggtataa
     1921 accacctgaa aattaggtat tacaaatacg aatataaaca acattgataa gatagataat
     1981 attataagta ttattggata gattgaaata gttattattt tcatttttaa tttgtattct
     2041 ttatcataat aattagataa atcgctcatt attatatcta agtttccact agtttctcct
     2101 gcttttatca tacttataaa gaatttagaa aatgtacctg ttatttgaaa tgattctgct
     2161 attgagttac ctttttgaat atgatttgat actatactaa gcaaattctt tactttttta
     2221 cttgactgac tctcaatgat ttcaaatatt tttgtaattt cacatccaga ttctagcagt
     2281 atacctatct ctttgcaaat tactctaatt tctttatcac tgattctatt taatactaaa
     2341 taaaactttt ctaaattata aaatatattt ttagataatc tcatgtacta taataccttc
     2401 caaattatat taattacaat taaataaact taatattttc taatttaatg cacttaattt
     2461 tatttcactt aaatataatt tacactcaaa catcttaatt aaaatagatt aaataaagac
     2521 aactgaccaa acgaagttta attatgcaat tcagttaaat catttcattt actactaaac
     2581 actcttctaa tgtcgttatt ttttcattta ttagtctttt acaactatct tcaaatgtta
     2641 tcattccatt attttgtgcc acgtctttaa tagtactaga atcctccata tttcttatac
     2701 atgtctttat atcatcatta atttctaaga tttcataaat tgctgttctt ccaaagtatc
     2761 ctttatcatt acatttttca caacctacag ctaccttagt atttacatct ccactaaaat
     2821 tatcttttat cataatctca tggctacagt gattacagac ttttctaacc aacttttgag
     2881 atataacacc tataagagag gcatttaaaa gatatggtgg tatttgcatt tctagtaatc
     2941 tagctatgga agatactgca tcattagtat gtaaagtact tataactaaa tgacctgtta
     3001 tagctgctct tactgctatc ttagctgttt ctgcatctct tatttctccg accattatta
     3061 tatctgggtc ttgtcttaat atagctctaa gacctacatc aaagctcaac cctacttttg
     3121 aatttacttg aatctgactt ataccatcca tcttatactc tacaggattt tctatagtca
     3181 ttatattttt atttattcct cttaaatcat ttaataaaga atatactgtt gtagtctttc
     3241 cactacctgt aggacctgtc actaataata tccctgctct tttatttatt atacgattta
     3301 tagattttat agcttcatct gaaaaaccaa gttctaattt gtcttttaaa aaagtatctc
     3361 tattgagtat cctcaaaact attttttcac catagacagt tggtattgat gaaactctaa
     3421 tgtcaattaa atttccacct aactttatat ctaccctacc atcctgagga agcctttttt
     3481 ctgttatatc cattgatgcc tttaatttta taaccgtaga caatgatgga tagacatcaa
     3541 ctaaaaactt tgatacttcc tttagttctc catctaccct cattctcact attaaatgtt
     3601 ctttaaatgg ttctatatgt atatcacttg cattttcttt tatagctttt tctaaaagat
     3661 tttcaaataa tttttttgca taatcctcat caagttcaaa gctattatag tatttttcaa
     3721 tcttttcact tataagttct tttttctcct tcttcaatat aatttcttta cctgtagcta
     3781 atctcaagtc ctgtaaagca taaatatcct catcttctat ttctacaact aaattatcat
     3841 cttgaaactc aactggaaat acattatatt tttttgcaag tttttctggt attaacattg
     3901 gtaaactgct ttcctctaat ataatcttaa tcacccttta tatactttaa tttattaggt
     3961 atattcattt ttttcataga gatattttct tcagttttta tgcttatttt cattggataa
     4021 ttggtttgta tgctatctaa atatttactc cctttatcta cataattaga tagtttctcc
     4081 ttatctccaa tgcactttat ttcatatggt tgtgctacag gaactccatt tatatttata
     4141 tggcttcctg caagtacaac ttctgaatat tgattaattc tttgaccatt tatttctatg
     4201 tattttcctc cgtttacttt taattcattt ataactttta caagaatctt attatagtct
     4261 atactattag ctatattccc tgttttttca tctatagcat ctatacttaa tattatccca
     4321 ctacccttta catccgaata tgatagacta tctctaagtg tttctatttc tgatatttct
     4381 tcagaatttt tttgattttg tttcattgaa ttaatttctt tatctaactt atctttctct
     4441 ttggttaatt cttttataga ttttcttgtc atttttattt cttttcttat tgacttgtct
     4501 ttgcttgtat aaactgtttc ttcattgcta taatctctaa atatggttcc aacaagtata
     4561 cctaaaatag tacttgctag tattataaac acatattgtt ttttcatact gtattgctcc
     4621 ttacaaattt atttttatat gataattttc tttaattaaa tattaaacat ttacatatgc
     4681 ttattatatc aaagttatat attaaaattc atctttagta tataatatca tacattgaac
     4741 ttctaccact aatttaccaa tatcaagtat gtatttttag atgttcttaa aattatataa
     4801 actttcaata aacattgttg tgatattata aaaaaacata aattagaaaa ggagataata
     4861 attatgaaaa aacaaataat gactttggag attattaata attatttttt atcaaaaaag
     4921 taaaatggca tagcctcaaa gctacaccaa tttactaaaa attatattat aattaacatt
     4981 aattttaaac atatgcgttt ataagttttc ctcatacttt aaaattcttt taattaattt
     5041 atctcttaat ttccctactt catcaaatct catattagga ctatagtaac tttctataat
     5101 atcatgttta gacttggctc tcttaagatt tagcactgcc caacaaatta aatcatctaa
     5161 aactttttca tcaaatttaa tatcttcttc atatttaagt agtttttctc tatttaaaaa
     5221 ctgatttaaa tcaattgtat cttcttcttt aaaagtcgta cttatagtta tacctatgtc
     5281 taaatcagtt aatagtacag tttcaatttt ctctggaata agtggataat gataaacttc
     5341 tacatttaaa cctttttcaa cagctttctt atatactttg tttaataatg tagttttccc
     5401 agttcctatt tctccagcta aataatagac cttatttaca tcttttaaaa tgttttctgt
     5461 ataatctaaa caccctacag gagttatagc tgaaccaaat aaatgctttt ctctttttag
     5521 atttcctgtg cttttaactc catcaaataa cctttttata aattcttctg tgaccaaatt
     5581 cactttgcca taatccatac aatcagaata tttttcttct atgtctctaa agattggttc
     5641 tgctgatttt aaaaacttaa atgccctttt aaatgaatta ctaatatctt tatcgtaaac
     5701 tgatatttct tgtttattct tttctaattc ttctgtattc cagtaatcac ctaaattaat
     5761 tatctcatcc aaagctccag gtagctttgg gtccatagta tgtggagcag ttgcatccat
     5821 aagtaccact cctaatttct ttataactaa accatctaac gaacttgggt cagaaggaca
     5881 atgatataaa tcaatatcat atcctctatg caaaaattct ttataaattt ttttcataag
     5941 tgaagatttt cctactccag gtccaccctt taaataaaaa actcttttta cattttcagg
     6001 aattatatag tcaaaaaaag aatatgagcc ttgtgatgta tttccacccg ggaatgcctt
     6061 ctttaccttt ccttccatat tagcgcctcc tcataattta caatatctct taattaagat
     6121 attcatatta taagaatttt attacagctt tctgattttg acaaatttaa aacttgccat
     6181 ctaatactaa taacaggtta attttatcta attattatag attctcatat tctcatactg
     6241 taccttattc tatctataaa tacaatttaa gtgtccatat tgaaatattt gtattgtaac
     6301 atagctggat attacttaaa tccaattgtt tccattataa ttttatgtta aaataatatt
     6361 acaaaataca tctgtttttc ttcataaacg ggtgaaattc cctatcggcg gtaaaagccc
     6421 gcgagcctta tggcataatt tggtcatatt ccaaagccga cagtaaaatc tggatggtag
     6481 aagaaaatag tatatgagta cctttatgta attttacatg agtaatctat acaaatcctt
     6541 caactaccgt atttattcat gaaattagac acattcaagg tacctaatat acaggtgctt
     6601 tttttgttgt ttattttata attatatcgt acttataaaa tctattaaga ttggagtgtt
     6661 atcatgaaac aaaaatggat agtattgatt atcatctgta ttggtgtatt tatgtctact
     6721 cttgatggaa gtatactaaa tatcgcaaat cctacaattg ctgctgactt taaaattaac
     6781 atgtcacaaa ttcagtgggt tgtaacagct tatatgcttg ttgtaacagc tactatgtta
     6841 tttttcggaa aacttggtga taaagtaggt tcaaatcgtc tatacacatt aggatttttt
     6901 atatttacaa taggttcttt tctgtgtagt atgtcaaaca atttatcaac attaatttct
     6961 tcaagaatct ttcaggcagt tggagcaagt attttaatgg ctactggact tggtatagtt
     7021 tccaatgcat ttccagcaaa tgaaaaagga aaagctatag gaatcactgg agctgtagtt
     7081 ggtataggaa atatgagtgg gcctgtaatt ggaggtataa ttttagaaca ttttggatgg
     7141 ccttctattt ttattatcaa tatacctatt ggtattatag cagtctttct tggcattaaa
     7201 tttttaccta aacctgtttt ggatgagcaa aataagagct ttgatattcc aggtcttcta
     7261 cttttcgcat cttgcactac gcttatatta cttgcaatga atgagaatgg taatacaaga
     7321 ttgtatttag gtataacagc acttataatc ttcttactgt tagcattaag agaagtaaaa
     7381 tttgaacaaa gctttattga cctacctcta tttaaaaata gaaactttac tgtaggcaat
     7441 gtcattggag tagcttgtta ctttcctcaa atggcagtat cttttctact tccattttat
     7501 ttagaacaat taaaaaattt atctcctatg atggctggtt atgtaatgac tgtacatcca
     7561 cttattatgg tacttatagc acctattgca ggttcacttt ctgataaaca cggtgcaaaa
     7621 aacattctga cagcatcttt ttcatttatg accatctcat tagttggaat ggcactttta
     7681 aaagcagact ctccattgta tcttttaatt gtatgtttag ttatttttgg acttgggcta
     7741 ggagcattta gttcaccaaa caatagtagt attttagcag atgttcctcc tcaaaaacag
     7801 gggtatggag gcagttttct tgcaactatt cgtaatttat catttgctct tggaacagca
     7861 ttttttagca gtttttttgc acaaagtctt gcatacaatc aaaagttcaa aagtcacaca
     7921 tcagcctatg ttatagctag caatcagtct tattggattg cagcatcagt gtgttttata
     7981 gggcttatac taactgtttt ctttatgaga aaaacagata aatctatttc ttaaatgcta
     8041 tttattgctg actacataag tcaataattt tttagttgaa ctaatttata ctaattttcc
     8101 cataccttac taaaatttat tagtatttag caaaattact gatatttatt gtttataaac
     8161 aataaggatg cccaaaaaag ttcattaaaa ctattttaag gcatcctata ttttgcttta
     8221 atattaatat aattaaaacc aactattatt acatttatta ttcaaataaa tcttatatca
     8281 acatttaaca ttctaaattt aatctactgt tatttcatat ccatgttgat tgtattgatt
     8341 tacaatttct tcccataatt cttcaacacc agtctttttt agtgaagaaa caggaattac
     8401 tttttcgcta ctttccattt gtaatttttt tcttattata cttatgtgtt tttgatattg
     8461 ccctctagat attttatctg ccttagttgc aataactaca cagttatatc caaagtgctt
     8521 tatccattca tacataagct tatcatctgc tgtaggctca tgtctaatat ctacaagtag
     8581 gaatatagaa cataactctt gtctactttc tagatatctt tccattatac ctccccactt
     8641 ttctttttca gacttagcta tctttgcata tccatagcct ggaaggtcta caaaataaaa
     8701 ttcattattt attaaataaa aatttatagt tctagttttt cctggtgttt gactagttct
     8761 agcaaagttt cttctgttaa gcaatgtatt tattattgat gattttccaa catttgacct
     8821 accagccaat gctatctctg ggattccttc tgctggatac tgtgatttat tcacagcact
     8881 catagttatc tctgaacttc taattttcat tattttcacc actctttaat aaagcttgtt
     8941 ctaatacttc atccatatgt tctacaagca caaactccat tttttctttt acattttctg
     9001 gtatttcatc taagtcagct tcacattctt taggaattaa tacttttgtt ataccagctc
     9061 tatgagctgc taataacttt tctttaactc ctcctactgc taagactcta cctcttaaag
     9121 ttatctctcc tgtcattgct atatttccag gcacaggtct tttagtaagt gctgagatta
     9181 ctgcaagagc catagttata ccagctgatg gtccatcttt aggaacagca ccttctggaa
     9241 tgtgtatatg aatatcattc gtcttataaa aatcagggtc tatgtcaaac ttgtccacta
     9301 ttgaccttat ataagagata cctgtcttcg cagattcctt cataacatct cctagttttc
     9361 cagttaatac aatttctcct ttacctttaa gtacgtttac ttctacttca agtgtaactc
     9421 caccaacttc tgtccacgca agaccattta ctaatcctat ttgagggtta acttctgcaa
     9481 gttgatactt aaacatatct tttccaagat atgcctctag gtcagactta tttataacaa
     9541 cctcttcaag agttgggtct tcaacgtatt ttttggcaac ttttctacaa atcttaccaa
     9601 tagttctttc tagtgttctt actccagctt ctcttgtata atggttgatt atacttctta
     9661 aagtttcatc atctattttt ataaagtttt cttttaaagc atgttcttta atctgttttg
     9721 gaagtaggta tttttttgct atatttagtt tttcttcttc tatatatcca gatacttcta
     9781 ttacttccat tctatctaat aatggtcttg gtatattacc tagactattt gctgttgtaa
     9841 cgaatagtat ttttgataaa tcaaaaggta tttctaagta atggtccaca aaatccttat
     9901 tttgctctgg gtctaatact tctagcattg ctgaagctgg gtcaccttta taatctgctg
     9961 ccatcttatc tatctcatca aataagaata caggattttt tgtttgggct tcttttactc
    10021 cattaattat tcttcctgga attgaaccta cataagttct tctatgacct cttatttctg
    10081 cttcatctct aactccaccc aaagatattc ttacaaattt tctatttaat gctctggcga
    10141 ttgattttac tatagatgtc tttcctgtcc ctggaggacc aactagacat attataggac
    10201 ctttaagtga cttagcaaga gttcttatag ctaggtactc tagtattctc tccttaactt
    10261 tttctaatcc ataatggtct tcatctaata tatcttttgc cttagttata tctaatttat
    10321 cttttgtctc tttattccat ggcaatgaga atatagtatc taaatagttt ctacttacag
    10381 atacatctgg agacatagaa gatatttttg aaaatttatc tatctctttt tctattttct
    10441 ctttagttgt cttaggtgct tttatttttt taagcttttc tctatactca tctgcttcag
    10501 agtttatatc ttcttcttca cctaattctt tctgtatagc ttttaattgt tctctaagat
    10561 aatattcttt ttgaacctta ttcatctgtt tttttactct taaagttatt ttcttttcta
    10621 tttttaatat atctatttcc tctaataata tagaataaat aagttcaagt ctttttctaa
    10681 tatcaaattc ttctaatatt tcctgttttt ggcttgattt tagatatatg tttgcagcta
    10741 tcgtatctat aaatctatct acatcttcta tatcagctaa agatattaat atttctggtg
    10801 ataccctatt tccaatattt atatactctt caaaggcatc aaatacattt cttacaaatg
    10861 cttcaatttc aacttctgtt tcactatcca aattatcact atcaaatact atttcttcta
    10921 ttactgctct gaaatatcca tcttcttgtt ctattttctt tactctaccc cttgatacac
    10981 cttcaaccaa aacacgaact gtatcccctg gtaattttat catttgctta actttacaga
    11041 ttgttcccac atgataaaaa tcctcttctc ctggctcatc tacctctgct tctttctgag
    11101 aagttaaaaa tataagttct tcatccatca tagcttggtc taaagcttta agagatatct
    11161 ctctacctat atcaaaattt aatatcatat atggaaatat agctaatcct cttagaggaa
    11221 ttagtggtaa ttcatgatct attttagtat aattttgttc catcaacatt actccttttc
    11281 tagtctaatt gctttgcttt taatattata tatttgaaca agttaatttt caacattaat
    11341 gataaattct tagaatacta tcatactata ttatcttttt attatgatat aatattaata
    11401 ttagtatact tagatgttat ttatcttttg gaggtttcta ttatgttgaa attgttttta
    11461 acctttttaa aaattggtgc gtttacattt ggtggtggat acgctatggt tcctcttata
    11521 caagatgaac ttgtaaacaa acaaaaactt ttagagcagg aagaatttat tgattattta
    11581 tccatagctc aaagttatcc tggagtactg gcagttaata tatctgtatt attaggatat
    11641 aagctttatg gtataccagg agttcttata tgtaccttag gctctgcttt accatcattt
    11701 tgtatagtct tagtcttatc gtatctatac tttaaaaatt catcatctaa aattttagat
    11761 ggatttttta aaggagttgc gcctgttgta attgctctta tattgtattc ttttacgagc
    11821 atgttcaaga aactaccaaa gtctaaaact aacatactac tattaattat agcagtttta
    11881 tctgttggtg tattcaatat aagccctatt tatttaataa ttggaggagg tgtatatagt
    11941 ctatgtcaga aataatacta ctcttcttaa tattccttaa aataggggca tttagttttg
    12001 gtggcggtta tgccatgcta ccatttatcc agcaagaatt tatatctaaa tatcacttta
    12061 taactaatca agagtttttg gatttacttg ctatatcaca atctacacca ggtcctgtag
    12121 caataaatag tgctacattt gtaggattta agactgctgg cttctttggc tctctagctg
    12181 caactattgg tgttacaatt tttgcattaa taggtctaac tattatatca aagttatttt
    12241 ctaaatttaa aaataataaa aaggtagaaa acttattgac agttcttaga ccgattactc
    12301 taggattcat attatcagca gctttttcat ctgctaaaga agttaattgg gatttatatg
    12361 gaataattgc atttatagca tcattttatc ttttatataa aggaaaaatt ggttccattg
    12421 caattgtatt cgtatttggt attctaggta tattactcac atttttataa taatgatacc
    12481 tattttatat ttagcatatt agcataaaaa aaggagcttt taaagctcct tttcatcatc
    12541 ttatgcactt tcctcttgat ccttaagtac tatgatagga tttactgagt cttcgttcac
    12601 agatttctct gttactataa ccttctttat attatctcta gatggtactt caaacatagt
    12661 ttccatcatt acgctttcaa ctatacttct aagtcctcta gctcctgtat ttctttctat
    12721 ggctttttta gcgattgcac gaagagcacc ttcttcaaat tctagctcaa catcatctaa
    12781 ctccaataac tttttatact gttttactag agcattttta ggttcctgta atatctgcat
    12841 taatgcatct tcatccaaca actctaaagt agctaaaact ggtattcttc ctataaactc
    12901 tggtattata ccatatttta ataaatcctc tggaagtact ttttcataca acttccctaa
    12961 atctaattct tttttacttt ctattttagc tccaaaacct agtgttttat ctccaccacg
    13021 tttttgaatt atcttctcta atccatcaaa agctccacct aatataaata atacattagt
    13081 agtatctatc tttaaaaatt cttgatgagg atgttttctt cctccttgtg gtggaacatt
    13141 tgcaacagta ccttctaata tttttagaag tgcctgttga acaccttcac cactaacatc
    13201 cctagttata gatgggtttt cagattttct tgcaatctta tctatctcat ctatatatat
    13261 tatacctctt tcagcttttt ctatatcaaa atcagcagct tgaataagtt ttaaaagtat
    13321 attttcaacg tcttctccaa catatccagc ttcagtcagt gatgtagcat ctgccattgc
    13381 aaaaggtaca tttagagttc ttgctaaagt ttgagcaagc aatgtcttcc cagaacctgt
    13441 aggtcctaat aaaagtatat tactcttttg aatttctata tccttgctac tagatttttt
    13501 actatatatt ctcttgtaat gattataaac tgcaactgat aaagcttttt tagctttttc
    13561 ttgacctatt acataatcat ttagaatctc catcatttcc tttggttttg gtaaagatgt
    13621 agtatcctca tctatagtat cttctatctc ttcttgaata atttcgtcac aaagttcaac
    13681 acactcgtca caaatatata catttggacc tgcaattaat cttcttactt gatcttgatt
    13741 tttaccgcag aatgaacact ttaattgtcg tttttcttca tatttagaca tattaacacc
    13801 tctctctatc tacggtctct ttgtaaatac ttcatctatt aaaccatatt ctttggcttc
    13861 tagtgcactc atgaagttat cacgttcagt atccatttta attttttcta atggttgacc
    13921 agttctttca gataatatct catttaaagt ttcttttatt tttaaaattc tctttgcatg
    13981 aatttctata tctgttgctt gaccttgagc tccacctaat ggttgatgta tcattatttc
    14041 actatttggt agtgctagtc tttttccttt tgctcctgct gccaacaaga atgctcccat
    14101 agaagcagcc atacctatac atatagtact tacatctggt ttgatgtatt gcatagtatc
    14161 atatatagcc attccagaag ttatacttcc tcctggagaa tttatatata aatgtatatc
    14221 tttgtctggg tcttcagctt ctaaaaataa tagctgtgct actataagcc ctgctgtggc
    14281 atcgttaact tgatctccta agaagattat tctgtctttt aatagtctag aaaaaatatc
    14341 ataagatctt tctcctcttc ctgtttgttc aacaactaca ggtactaatg ccatggtttc
    14401 ataccccctt tattctatat aatttacagt agcttttttt aagctactgt aaattattat
    14461 acgcatttaa ctaaattata tttgcacatc attatatact aaacatttca taacgtcaac
    14521 tatattagtt agcgtttatt ttattaagct attgttgcat tatcaactag taaatctatt
    14581 gtttttctta tttttatttg accttttata tcttctttgt cagcatctct taaagaagct
    14641 tctatttttt ctacttccat attgtaagca gaagccattt tttctaactc agccttaaat
    14701 tcttcttctg ttgcttcaat tccttcagct ttagttattg cttctaatac taaagaagac
    14761 ttcactaatt ttttagcatc ttcttttctc tcttctctta attcttccat agttctacca
    14821 gtcatttcta ataattgttg taatccaaat ccttgatatt gtaattgata gttaagttca
    14881 tttaacatat tatctatttg atgttgtatc ataacttctg gtatttctat ttctgtattt
    14941 tcagcaacct tttctactac tgaatttcta gtttctgcat ctgctctatt tttagcttct
    15001 tcttctaact tagctcttac atctgctttt aattcatcta aagaatcaaa ttcactagta
    15061 tctttagcaa attcatcatc aagtgcagat aattctttta cttttacatc atttattttt
    15121 acattaaata caactggttt tccagctaaa tcttgtgagt gatattcttc tgggaaagta
    15181 acattaactt ctacttcttc accagctttt ttacctacta attgctcttc aaatcctggt
    15241 ataaatgtgt ttgaacctat aactaagtta taattttcac ctttaccacc atcaaaagct
    15301 acaccatttt caaaaccttc gaagtctatt atagctgtat ctccatcttc taaagctttg
    15361 tcttctacac ttactaatct ggcatttttt tcaaccattt cttgtagttt agcatcaaca
    15421 ttttcgtcac ttacagtatt ctcaacctta gctatttcta tacccttgta gtttcctaat
    15481 tcaaactctg gtttaacttc tacattaaga accataacca acccattgtc tttacttatt
    15541 tcttctatat ctaaatctgg attgtctatt gggtctatgt ctaattcctt gatagcttct
    15601 ggataaactt ctgggaataa tatttctata gcatcattat agaatatacc ttttccatat
    15661 tgagtctcta taacaattct tggagcttta ccttttctaa atccaggtat attgtattta
    15721 tttctgcttt tattataagc cttatttact gctgcttcaa atttattgtt atctactgtt
    15781 atttttaaag taactttatt accctctttt ttaattagtt ctgctttcat ccttctaatc
    15841 ctcctagtga tatttatgta atacccgata acggtattat aattcattct atttataatt
    15901 caaccactta attatatcac ataagttttt ttatttacaa gtgttccatt aatatcgttg
    15961 tacttatttg ataaaattta attttttgag cgttttttgc cattttttta gataaataca
    16021 cctttatatg cgtatttctt ctatatttat gttatcttct tctatatcca taatgacaaa
    16081 acttttatac gatactcctc ttggaagtga agtacttcca ggatttatgt aaagtacacc
    16141 atctttaact tctctaaacg gagtatgggt atgaccaaat ataactatat cagcatcaat
    16201 ttcagttgcc tttttttcta acatatgcgt tccatatttt acaccatact tatctccatg
    16261 acataagaat atggttttat ttgccacttc aaagacaact tcttcctcaa cagcatcaaa
    16321 gtcacaatta cctttaactg caataatacc tacattagtc atgctatgaa tatatcttga
    16381 atctgtaaag ttatctcctg catgaattat taggtcacat tcttttaaat atggtattgt
    16441 tttttccata tttttaatca tcatatgtgt atcgcttact atgcctattt tcattacttc
    16501 actacccttt ttataaactc ttgtttcata agttttagtg cgtttgctct atgactgatt
    16561 gaatttttta taacactagg tatttcacca aaagtcttat catatccttt tactataaat
    16621 aagctatcat atccaaaacc atttttacct ttttcttcaa atcctatcat tccttcacaa
    16681 attcctctta ctacaaattc ttttccatca ggaaaaacta ctgctattgc agagacgaat
    16741 cttgcatttc ttttgctcat tggtacattt tgcatagctt ttaacagttt ttttctattt
    16801 tcttcatccg ttgcatgttc tcctgcatat ctagctgaat aaacaccagg ttttttgcct
    16861 aaagcatcta cttctaagcc agaatcatct gatattgcta taagtttagt cttttttgct
    16921 attgttctag cttttatcaa tgcattatgc tcaaatgttt ttccatcttc tactatctct
    16981 atacctgcta aatcaacatc ttttaaagag tacactttat attcaaaatc ttttaagatt
    17041 tcacctattt cttctagctt atgagcatta ttagtagcaa tcactacttc attaccataa
    17101 tccataccca atatctcatc tgctatttca cctaaagctt ctctttgaag ccttattaat
    17161 tctttattcc ctttttcacc taactctaac aataaattta aatctgctct cgaaaaaggt
    17221 ttctcttctc ctgtcccctg tacttctaca aactcacact tatcagtcat tattatattc
    17281 atatctacct gtgctttaga atcttcttca taacataagt ctagcatgtg tattccattt
    17341 acaataccaa cactaattgc agatacaaaa tttgttattg gcatctcctt tattgtcttt
    17401 gctttatata atttatatat tgcttctgca actgctataa atgcccctgt tatagatgca
    17461 gttctagttc caccatctgc ttggatcaca tcacaatcta cccaaatagt tctttctcct
    17521 aatttattca agtctacaac agaccttatt gctcttccta ttaatctttg tatttcttgt
    17581 gttcttccat caatttttcc cctagatgca tctcttatct ttctttgttg tgttgctctt
    17641 ggtagcatag aatattcagc atttatccat ccagtaccac tatttcttaa aaaaggagga
    17701 actttttctt ccactgatgc agtgcatata acctttgtct ctcccatttc tataagaaca
    17761 gaaccttctg catactttgt ataatctcta gttatcttta caggtcttat ctgttcgtta
    17821 cttctatcat aacttctggt cattttttat atcctttctt atattttttg ttttttataa
    17881 atttattttt ataattttta attttgtcaa atattaatta ttgtcatttt tcgtaatctt
    17941 attagcttac tgaactattc tatcattaat tatagtagtt ttctactaaa ttaagcgact
    18001 ttttaactct ctaatacagc ttttacttca tctttcgatg ataactttaa taattctaaa
    18061 aatctctctt catctataac tttaattcct aaatcatttg ctttagtgag tttagaacca
    18121 gcttcttcac ctgccaaaac aaatgttgtc gatttactaa cactagatgt agcttttcca
    18181 cctctttttt ctatcatatc ttttgcatca tttcttttta atgtaggaag tgtaccagtc
    18241 aatacgattt tcatcccttc aaatattttt tctacttttt cacctgtatt atccaatgat
    18301 tttgtattta cgcctacatt ttttaacttg tttataacgt tcatattttt gtcttcctta
    18361 aaaaacttga caacgctgtt tgccattatg cttccaaatt cctctaattc ttctagttgc
    18421 tcactagttg cagaaattac ttcatctaaa tccttaaagt tatatgcaag tatcttagct
    18481 gctttaactc ctataaattt aatacctaat ccatttataa gtctccataa atcattttct
    18541 tttgattttt caattgaatt tattaaattg tcagcagatt tttcgcccat tctctctagg
    18601 tcaactacat cttccttttt tatataatat aaatcagata catcttgaat aattttttca
    18661 tttaatagta gtgtaattat agattctccc aatccatcta tattcattgc atcccttgac
    18721 acaaagtgta taattcccct tcttatttga gctggacaag acatatttat acattttaca
    18781 gcagcttctc cttctaatct tattgttggc tctgaacaga caggacactt ttctggcatt
    18841 ttaaaatcta tttcatctcc agtacgttct tcttttataa cttctaaaac ttgaggaatt
    18901 atatcccctg ctttttgaac taatactgta tcatttattt ttatatcttt ttctcttata
    18961 taatcttcat tatgaagtgt agctctactt acagtagtac ctgcaagtct gactggttca
    19021 agaattgctg aaggtgttat tgtaccagtt ctcccaactt ctactattat atctaatatt
    19081 ttagttttct tcttttctgc tgggaattta tatgcaattg cccatctagg acttttggct
    19141 gtatatccca tttcgtctct ttgttttata ttgtttactt ttattaccat accatctatt
    19201 tcgtagtcta aattacccct gttatctgtc cagtattcaa tatgttcaat aacttcatct
    19261 atacttttac aaactttata actctgactt actgaaaatc ctagtttttc taagtactct
    19321 agtgattcac tgtgactttt taaatcatat tcttctatat tttccatatt aaatacaaat
    19381 atatctaatg gtctctttgc agttagtttt gggtctaatt gtctcaatga accagctgca
    19441 agattccttg gattagcaaa taatggctgt tcttgttctt cttgttgttc atttactttt
    19501 ctaaaattat cttttgatat atatacttct cctctaacta ttagttcttt tttatcattt
    19561 atcttaagag gtatgctttt tactgtcatt aagttttgag aaatatcttc tcctacaatt
    19621 ccatctcctc tagttgctcc tttttcaaac tctccattat tataagttag acctacagaa
    19681 aggccatcta ttttaaattc aacaacatat tcaacatttt ctcccacata ttcttgaacc
    19741 ctcttatcaa aatctctcaa atcttgctct gaaaaagcat ttgataaact caacatagga
    19801 gttttatgta caatttgatt gaatttatct aatggcttac ctcctaccct atttgttgga
    19861 gaatcaattc tctttagttc tggatgttct tcttctaaag aaattaactc cttcattaac
    19921 ttatcgtatt cataatctga tatttctggg ctatcttgat tataatacct ttcattatgg
    19981 tagtttattg tctctataag tttattaatt tcatctttta cgctcacttt ttgcacctct
    20041 tcatattttt taaattaaat tattttgaaa tttccctata attaattatc tcctatttat
    20101 atatttttta gtatatcact tttttgactt taaatttatt acaaaaaaaa ctcttattag
    20161 tatctctaat acgactaata agagttttta tttttatcaa aattttttat ttttaatagc
    20221 ctcttgatat gtctacaaca ttatctacag gtttattctc cattatatag tgtttcaaat
    20281 tattataaac catattaaat gttctctctt tgtttttatc tgacacccaa gaattatgag
    20341 gagttactat gacattttcg cactcccata acttattatc cttgtttaaa ggttcacttt
    20401 caaatacgtc taatgctacc cctctaaact tctctacaca ttcttctaaa tctttctgat
    20461 taactgtatt tcctctacct acatttataa atacagagcc atctttcata agtttaaaca
    20521 tatctttgtt tactattcca tctgtacttt ttgtagaagg cattgctaca actactatat
    20581 cacattcttc aaatacaata ttcatatcct cagtggcaaa acacttatca aagtattttt
    20641 tactacttcc atcagtattg acaccccata tttctagacc aaaagctttt aatctttttg
    20701 ctgcttctga agatatagta cctgttccta aaaatccaac tctttgtcca tacaattctg
    20761 atacagaaaa atccatcttc cattctttat tttgttgttg tttaaaaaat tttgcactat
    20821 ttttatatat ttctaaaatg tacaatacaa tactttcacc aataggtaca ctatatccgc
    20881 ctctattatt agctatctta atatttctct tcaatacctc atcttttgga agttggtcta
    20941 tacctacact agttgtctgt atatattgta aatttttcat cttagaaata tctaatctct
    21001 caaatggatt gtatgtaact agtacatctg ctgtatttac atcctcatta ttttcaatac
    21061 tatcttcaga gtaatgtatg acatcatagc ctaattctct tattttatca aatttttctt
    21121 ttccataatt tattgtaaat aaaactttca tctcaaatct cctccatcta cccacattta
    21181 aaatgttata ttctaaagta taatatgata ctcaatgtac attagttaaa attactagaa
    21241 gatatcttaa atttacttat gttcaattgt ttttcctaaa gacatagcat tacgttcttg
    21301 aactaaattt tctattaaat tattcaatgt ccaatcaata gatgtctccg ttaccatagc
    21361 tgtaaaaggt ctatttattc tcactttttt tagcccttct aaaaatgcat ttacttgtgt
    21421 acttggagtg ttattaaata ttacagactt actcttaatc ccattttcac attcatttga
    21481 tatatttcca tctaaaatgt ctcttattat gttctctcca tttttatcac ttaagatgat
    21541 atggtctctt attccagcaa agttacttac atttttaatt aatgataatt cttttttatt
    21601 aaagtttaca atcataatac acttcctatt gtgtatttta tctaggttat tattatttat
    21661 tttttcaaat gacattttat ccatcctttc atttatttat atactttaat ttaccccata
    21721 catttaaaat aatcaatttt tatagttaat ttttatatta atacccaaat tatatttaaa
    21781 tagttaggct atccaaaact aatatataaa attagaataa ttagattaat atctacaaat
    21841 cataacctaa aggagagata ccatgaatac attactaaac aataaattta gcattaaaaa
    21901 cttttcaaat gcttgggtta tagtacttgg ttgtatgttg atacaagctg ttccatttag
    21961 tattgtagca agtatacagc ctcaatttat aagttatgtt gtagaaggag aaggttttag
    22021 tttagcttct ttttctctaa tattcactat tggtactatt gcttcagcaa ttgcatcacc
    22081 ttttataggt gtattatttt ctaaagtcaa tattaagata ctatatttat taggttgtat
    22141 aatttctagt attgcttttg ctactttttc tatatgtact gaactatggc aattttatac
    22201 tgtatcatgt attgttcaaa ttggcgctac aattatatct gctataggaa tacctgtttt
    22261 aataagtgct tggtttgatg aagaaagcaa aggaaaagct ctgggctttg cttttgctgg
    22321 tggttctatt ggtagtatat ttctacaacc acttgcagtt cgcttattag caactcaagg
    22381 atacagtcgt tcttatctaa tatttggaat tatagcatta atagttggta tacctgtaat
    22441 attattcata ctcaggatgc ctaaagatat taatgaaaag gtaaaatcta aaggtaaaaa
    22501 agaaaaagca tctgatacaa tttctccttc agtatggggt tatacattaa aagaagctaa
    22561 aaaaattaaa tatttttgga ttatgacgat tggatttata tttgttggta tatatgtttc
    22621 atcattatcc gtacaatatc caacttatct tagaaattat ctaaatccta ttctgatagg
    22681 aacagtaggc tccacatttg ctttatttgc tctcattggt aacttatttg gaggattatt
    22741 gtttgataaa ttcggtcaaa caaagccact tattatatct tttgtattgg ttcttatagc
    22801 aactttatca ttactattgg tagataaatc ttctgctttt gcatttatat ttgccatttt
    22861 gaaaggtctt tctgtatttg cttatatgat tggtcctgct tatttaactg gctctttctt
    22921 tggtcaaaag gagtttggta gcatatatgg aatcgtacag ttaatttttg cagtgggatt
    22981 tgcattaggt tcaacattat ttggattgtt agttgataat tttggataca gattttcttg
    23041 gtctattata attttatcag ttataattgc ctatacttta cttattataa cttctatagg
    23101 aatgaaaaaa ttgaataaag aaagattaga taaactaatg caataattga tttttagata
    23161 tataaatatt ataataaaaa agatggagct tttaatcata aataaaactc catctttttt
    23221 atattacaat atagctaatt ttctcttatt tttaacttta ctatctatat cctcatcctt
    23281 aactatagta aatgccttag ttggacaaac atttacacat gcaggtcctt ctggcgattc
    23341 tatacaaaaa tcacacttat ttgctgtcat tctttctcta aaacaaagct ttccttcatc
    23401 tacatctttt aatgtaacta tgtctattgc acctatagga catgctaaca tacaagtctt
    23461 acatcctata caattttctt catttatttt tattgtgttc ccaaccctaa ctataccacc
    23521 atttggacat gcctttgcac aaggagcatc ttcacaatgt ctacactgta ttggagcact
    23581 tacttctttt gtctttatga cagaaagttt tggattaaaa tttatttcat ctggattttg
    23641 gtaaaaaata tcttcatctg aatgtgcaac tacacatcct atcatacaag ttctacatcc
    23701 tatgcattta tcagggtctg ctataacaaa atagttcatc acttagccac ttcctttcct
    23761 atacaatatg tagtgtgtaa tagatgatga gatttttctc ctaatggttc taccaaatat
    23821 tcatcataca gttttttaat atcaggattc tcatgagatt ttctaattgc taaatgttca
    23881 tcatgcacat atgttgcttt tgttctattt tcataggcca attccttata ttcttctagt
    23941 aaaatttttg gttgacctcc accacttaca cacccaacag gacaagtcat aacttcaata
    24001 aaatctacat ctaattttcc tttttccaag tcttcaagca ctggtattac attttttaat
    24061 ccacttacta ctccaactct taagtctaag tctcctattt taatagttga ctttctaaaa
    24121 ccattttctc ctcttacttg ttttacttct acatcaggaa tactttctcc agttataagc
    24181 tcatatcctg ttcttatagc agcttccata actccaccag taacaccaaa gatagttcca
    24241 gctccactat aactacccaa aggactatca aatttttctt ctcttaaatt tttaaaatca
    24301 attcccatgt cttttattaa ataagctagt tctctagtag ttaatactac atctacatct
    24361 ctatatccac tagaatccat ttcttctcta tcacattcat attttttaca tgtacatggc
    24421 attatagaaa cactaaatat ctttgatgca tctacattat ttatctctgc accataagtt
    24481 ttaaatattg caccgcccat ctgctgaggt gatttacaag aagataaatg attagttaat
    24541 ttaggataat ttttttccat aaattttacc caagctggac aacaagatgt aaacattgga
    24601 agagttccac cttctgtcac tcttttaatt aactcactac cttcttccat tatagttaaa
    24661 tctgctgcaa aattagtatc gtagactcta tcaaaattta aagctctaag cgctgctgcc
    24721 atttttccag cacctaagta tcctaaataa tatccaaaat cttctcctaa agcaactcta
    24781 actgctggtg cacattggac catagtaaat acatttgggt cacttaaagc tttcttcacc
    24841 ttatctatat cacatacact gtatgctgca aatataggtt catttataga ctctggtatc
    24901 tctctttctt gttttttctc ttgtaaaaac tcaaatcctt catctataac tgaggcataa
    24961 gatttacaag tttgaacaca ttgaccacac ataacacatc tatctaaatt tatctcttgt
    25021 ggctttccct cttcaccttc aatagcattt actggacaga cctttgaaca ttctctacat
    25081 ccagtacata aatccttatc tatatttatt atcataaacg tcacctaccc ctgtatcctt
    25141 tatttatttg ttaacaataa actaagtgca gcttgtctat tcttagcttt tttgtcatct
    25201 attggagtaa caagttttaa tgcttcctga ggacatgcac ttatacaagc tattttttca
    25261 ttgtctttac ataagtcaca tttataagct atctttttat tttcatctaa aattacttgt
    25321 tctacttctt taccatcttc atattgtgga agtaagtcta tagctccaaa aggacatgct
    25381 agcagacaag ttttacatcc tatacacttt tcttcattta tagacataat attattttct
    25441 ttgactatag ctttttgtgg acatgagttt aaacaaggag catcttcaca atgtctacac
    25501 tgtattggca tacagaaatt gtcacctttt actaaataaa gtctaggtat aacaggtata
    25561 tcaactgttc caactgtata acccacatta ttatgctgat tatgcattgt aaaacaagct
    25621 acttcacaag ctctacaacc tgtacatttg tttgggtccg ctattacaaa agagcctatt
    25681 ttatttttca tattatccca cctttcaatt tgtatatttt caaccctcta gttactttca
    25741 tcatatatat ttatatattt cactattttc tcagtactta tatttcgttt acattttttg
    25801 cacaaattgt atgtgttatt atacacctta tcagtatcat caattaatac tccacagatt
    25861 tcacactttg aatatttttg ctcttctttt ttagtacaca tatacttatt ctcacttttt
    25921 gataactctc tgtggatttg cataaatatt catccgtctt cctcttacaa atccaataag
    25981 tgttatattt aattttttag aaagctctat tgataagtta gtcggtgctg attttgatac
    26041 tacaatagga atttgagtca tagctgcttt taatatcatt tccaatgata ttcttccact
    26101 tacagccaca attttatctt ttaatgttat gtcatttaaa acacaaaatc caatagcctt
    26161 atccattgca ttatgccttg ctacatcttc acaagtgact atttttttat caaaattaaa
    26221 tacagatacg ctatgtaccc caccagtatt tttaaataat tctgatgaat ttaaatttct
    26281 atccattata ctgtaaatcg tatcataatt tatttttata ttgctctcta cagaagcaca
    26341 tgttatatag tctagtgaat ttaaaaatat aatttgctca tcttgatttt tttgtttctc
    26401 ttgaatagtt acttttgcaa aacgttcact ctcatttatc tcaaatttta ataagctttt
    26461 tttgttttct atgtatcctt ttgttttcaa gaatcctact ataagttcct ctaaattata
    26521 tggtgtacat aaaaatgtat ttacatattt atcatttaaa ataaaactaa gtggatattc
    26581 tgcaattatt tcttcattat ctattgatac ttcatgttca tttattttgc ttacttctac
    26641 attaaaagaa tttaaatatt tgtagtccac tagactataa ctattttcaa aaactttatc
    26701 cttactattt tcttgtaatt tagtttttga gtatatattg ttcataaaat attagccttt
    26761 ctgcctttta taaatcttaa atctaataaa ttattagata tttgtagctg tcattttgtc
    26821 tttcaaactt tgatactctt ctcttacaca tttctctgct aattcttggt cttcaagttt
    26881 ttcaagttta acagcacaat acttatactc tggtgtttta gatatagggt ctaaatttgc
    26941 tattgtaagt tcattacaag ctccaaccca ccactgataa gtcatatatg ttgcaccttc
    27001 cttaactcta tcagtaactg ttgctcttgt tattacactt cctcttcttg aactaattct
    27061 aactaattca tcatcttcaa ttcctaattt ttcagcatca ttagaattta tttgaacacg
    27121 tcctggttca tcttctaatg aactaagtgt tctacaatta ccagtcattg ttcttactga
    27181 atagtgtcca acttctctta cagtgcataa gctaaatggg tattcatcat cttcaacttc
    27241 cataggaggt ctccattcag cagcgaacaa gttaccttta ccattaggag tagaaaactt
    27301 ttgaccttca tacagataca tagttccttt atcttccata ctttcactct tacaaggcca
    27361 ttgaacacac ccttgagttt ctatcttttc ataagtagca cctaagaaac ttggacataa
    27421 ttgtctaagt tcatcccata tttccttagt atttttataa ttcataggat atcccattgc
    27481 tgtagaaatt tcgcttataa tttgccagtc tggttttaca tccccttgtg gctctatagc
    27541 ttttctcatc aattggaatc ctctatctgc acatgtatat actccctcat gttctcccca
    27601 agaagtagct ggaagtataa catcagcgtg aagtgctgtc ttattcataa atatatcttg
    27661 aacaatgaca aattccaatt catctaatgc ttctctaact tctgatgcat ctgggtcact
    27721 ttgcactggg tcttctccaa atatataata agctttaagc tttttctctt ttagtactaa
    27781 gtctggaact tgagttaaac tataaccatt attaggagat agttttactc cccaagcttt
    27841 ttcaaacttt tctcttatct tatcatcagt tacattttga tagccaggat atacattagg
    27901 aagagctccc atatcacaag ctccttgaac attattttgt ccacgtacag gtcctattcc
    27961 aacgctttct ctaccaaaat tacctgtaag tagtgcaatt gatgcaagtc ccttaactac
    28021 atcaacagct tgaccaaatt gacagacacc cattccataa agtatcatag cttttttacc
    28081 ttttgcatat tctctcatag cttttcttat aagctcttct gggattcctg taatcttttc
    28141 agcatattta gctgtatatg gtttaacaat ttccttatac tcatcaaatc cttgtgtatg
    28201 attttgaaca aattctttat tgtaaagacc ttcttctatt aatacatttc caaaagcatt
    28261 tactaaaacc atatttgttc caccttttat aggtagatgt atatcagcaa ttcttgcaga
    28321 ttcagttact ctagggtcag taacaattaa tttagcacca ttttttttag cttttactat
    28381 tctatttgcc actattggat gtgaatctgc tccattatat ccaaatataa ataaaacatc
    28441 tgcattttct atctctggta tagaattaga cattgcacca ctacctaatg agtaagccag
    28501 accggctaca gatggtgcat gtcagacacg agcacaatgg tcaacattat tagttcctat
    28561 tgtagctctc ataaattttt gcattatata gtttgcttca ttaccaggtc ctcttgcaga
    28621 acctgttccc ataatagaat caggtccata tttttcttta atttcattta gtcttgaagc
    28681 tgtatagttt atagcctcat cccattcaac ctcttctagt acaccttgtt ttcttatcat
    28741 tggctttttt agcctaggtg ttagaatttt agggtcattt agaaaatccc atccataacg
    28801 accttttaaa cacaaacttt cttgatttgt tctaccatta gccggttcag ctcttaatat
    28861 cttattattt tttactacta gatataattg gcatccagct ccacagtatg gacaaactgt
    28921 taaaattttt ttctccatac aattcccccc ttagatttat cccctgataa gtaaaattta
    28981 tcttaaagat aatgagtgtt tttataatat gcaaaatcat aaatattttg caatactatc
    29041 tattaaattc aacaattttc tcacaattcc tcttaaattt taaaaaaaat atgtcataat
    29101 aatctttata tttcaaccat ttaattaaaa tattctagtt cttatgacat attttttaat
    29161 atactcaata tatattcttt aaacctattt agttttcact taaaacaata tttctgccaa
    29221 ttctattttt cattctatag tgagtaatta cagtaaatat tccacttcca atcagaatca
    29281 aatagtacat aaatccacca tataaaaata ctgcatatcc catcaaaggt tgagggaata
    29341 ctgatttgaa tatagtgaaa aatgctaatt cattagctcc tacattccca ggtgttggta
    29401 ttggagatat tgccatatat aaaaatgctt gtaatgtcag tatataaata tagctataac
    29461 cttgtaaatt aaatgcttta tatatccaaa atgaaacact aaagtaagca cttaattgaa
    29521 taaaagttac tattatagtt gaaattaata cctttttatt ctttgcaaaa aaatttattg
    29581 ctttactata atcatctata aaactttcta tggattcaat cttattatcc aaaaatttta
    29641 aaaacttaat ttttgataaa tgctttatag taattctcat aaaatatttt atttttttag
    29701 ggtttaaaat aactaatatt atcataatta agacaaatga attaatggct atccctagaa
    29761 acactagagg cataatcatt ttcatttgtt tttctaacat agcaaaattc attaatacaa
    29821 gtattgtaca ataaaaagta actactattt ggaaaataat agacttattg gtaacaactg
    29881 cacttgcctt actaagtggc attttacatt ttttaagtac atatatctgt actggttggc
    29941 ttcctgaagc aaaaggagtt attaaattat aataaaatcc cataatggct agcttaaaac
    30001 caataaatct aatattaact ttgtgagtgc tttctataat tatcttcatt actactcctt
    30061 caagcattat gtgacacatt attgccaaag cacctatgaa taaaaacttc ttatctacca
    30121 taaccataac atttgatagc attttaatat ccaatgtttt aaacactagg taaattgtta
    30181 tacctattaa caaaaataaa aatgtatact gtagtaaact ctttctaata ttctttagtc
    30241 tatccattag aaataaccat cctttgctca agatttatac taaatactat attattagta
    30301 tacactatga atcttaatat tttataacaa aataccttac aattttgtca tttacaaaaa
    30361 atattaatct ataaattaaa ttttaatata tagattaata ttttacaaac attttgtaaa
    30421 tatatataat attttcacta aacaacactt atacttatta cacccattta taccctatcc
    30481 cccatacagt ttctattgta taaatatcat attgttttaa tttgcttcgt atatttttta
    30541 tatgctctgt gatagtactt atatcacttt ctccatcaaa accatatacc gactcatata
    30601 ttctttcttt tgaaaatact tgtcccttat tttttgctag aaattcacat attagatatt
    30661 cacttttagt aaaattaatt tttttaccat caattaaaac ttctttgcct aaaaagttaa
    30721 attgaacatt tgagatagtt aataaattct ttttttctct actttctctc cttaaatgag
    30781 cagttatcct agcccttaat tctcctacac caaaaggttt ggttatataa tcatctgcac
    30841 ctataccaaa accatacata atatcacttt ccatattttt tgctgtcaaa aacagtattg
    30901 gacaatcaac cttatttctt atagttttac atacttcaaa accatccatc ttaggcatca
    30961 taacatctaa tattattaga ttaaaataat taaaatccat gttcatagcc ttttctacat
    31021 cactaattac ctttacttca tgtccatctc ttttcaacac attttcaatc aactttaaca
    31081 tatctaactc atcatctact actaatatct tagacataat aactcctcca tttatccatc
    31141 taatagcatt ctaatttata tatttaataa tatctttaaa attcacatta accat
//
LOCUS       DAEPXF010000026        31055 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain HB27
            SAMN13376389-rid12479423.denovo.027, whole genome shotgun sequence.
ACCESSION   DAEPXF010000026 DAEPXF010000000
VERSION     DAEPXF010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13376389
            Sequence Read Archive: SRR10518432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 31055)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31055)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001069624.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001069624.1
            Genome Coverage       :: 315x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/15/2021 01:54:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,165
            CDSs (total)                      :: 4,076
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 89
            rRNAs                             :: 10, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 10 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 19 of 69
            Pseudo Genes (incomplete)         :: 44 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 10 of 69
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31055
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13376389-rid12479423.denovo.027"
                     /strain="HB27"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="China: Hebei"
                     /collection_date="2017"
     gene            complement(51..212)
                     /locus_tag="KRL67_001085"
     CDS             complement(51..212)
                     /locus_tag="KRL67_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH1521984.1"
                     /translation="MNNFLLNVIAGVIASLIFCLICKVFLKVKSHSTRGKSKSGWEFD
                     FKIKFHKFK"
     gene            complement(553..993)
                     /locus_tag="KRL67_001086"
     CDS             complement(553..993)
                     /locus_tag="KRL67_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HBH1521985.1"
                     /translation="MSNLSAFLSQNAIKVDNVKYVASNRFLDKEGKPVEWELKVLSSE
                     EDEALRRKCTKRVKVIGNNGKHTGQYTSEIDYNSYVAELCVASTVFPDLKDAELQNSY
                     GVMGEAQLLKTMLTAGEYVNYTVKVNEVNGFDTSFEDKVEEAKN"
     gene            complement(1065..1535)
                     /locus_tag="KRL67_001087"
     CDS             complement(1065..1535)
                     /locus_tag="KRL67_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tube protein"
                     /protein_id="HBH1521986.1"
                     /translation="MAQTINAKDTVSAKKAECFITIEGKRYNFMQAIDLEAKMEKNKS
                     EVPILGRTTKGNKTTGSTNTGSATFHYNTSIFRELLYRYKETGEDIYFDIQVTNEDPT
                     SAVGRQTVVLKDCNMDSGIITKFDADGEYLDEDMDFTFEDWELVEKFNLLAGME"
     gene            complement(1552..2862)
                     /locus_tag="KRL67_001088"
     CDS             complement(1552..2862)
                     /locus_tag="KRL67_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath family protein"
                     /protein_id="HBH1521987.1"
                     /translation="MALGGGTFVTQNKVLPGAYINFVSATRATSSLSDRGIVAIPLEL
                     DWGIDEEVFQVTSDDFEKYSVKYFGYDYTHEKLKGLRDLFKNIRLGYFYKLNKGVKAS
                     CTIATAKYSGIRGNDLKVTVTTNIDDNAKFDVVTLLDNKKVDTQIAKVITDLQDNDYI
                     TWKKDATLEASAGLVFTGGTNGEAVTGAEYQAFLDKIESYSFNALGCLATTTEIKSLF
                     VEFTKRMRDKVGAKFQTVLYKKSDADYEGVVSVENKIKDKDLVESSLIYWAAGAIAGC
                     DINKSNTNKKYDGEFDVDVNYTQIQLEEALKTGKFIFHKVGDEVHVLEDINTFVSFTD
                     DKNDDFSSNQSVRVLDQIANDIATLFNEKYLGKVPNDKAGRISFWNDVVKHHKELENI
                     RAIEDFKTDDVSVELGNDKKTVIVSDAVKVINAMSKLYMTVSVS"
     gene            complement(2863..3039)
                     /locus_tag="KRL67_001089"
     CDS             complement(2863..3039)
                     /locus_tag="KRL67_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH1521988.1"
                     /translation="MSKTLSKEENYKFTKEQIVNSKRYVNRKDLLNAILKEDELYSFS
                     EVEDMINNFMKGVS"
     gene            complement(3032..3469)
                     /locus_tag="KRL67_001090"
     CDS             complement(3032..3469)
                     /locus_tag="KRL67_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH1521989.1"
                     /translation="MLNNIIDGISIKLDKTFGESYTIYSEDVEQGINEPCFFIVPLNP
                     SKVSYPSGRTLKKNSFDVHYFPKSNDKSFEINEVAEMLLEELEYIEIDGDLVRGTNMN
                     FEIVDNVLHFFVDYNYFTIKSNNTDKMDTVELFGGLKRGDKLE"
     gene            complement(3462..3887)
                     /locus_tag="KRL67_001091"
     CDS             complement(3462..3887)
                     /locus_tag="KRL67_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 gp10 family phage protein"
                     /protein_id="HBH1521990.1"
                     /translation="MARWGSVDFREFKRVCKKVEKLTKIDLDKFCKDAARELAARLLG
                     KVIRRTPVDTGFLREGWSGVAYARSLPVYKQGNNYIIEVVNPTEYASYVNFGHRTKDG
                     KGWVKGQHFLTISEMELQSQVDKIIEKKLLILLKGVFDA"
     gene            complement(3887..4234)
                     /locus_tag="KRL67_001092"
     CDS             complement(3887..4234)
                     /locus_tag="KRL67_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015984843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH1521991.1"
                     /translation="MVSKTRKAIEMLYRDKCTIVEYQPIKDPVTKRTNNKEVIVLENQ
                     PCKLSYKNIVSATEGKLAKLEQTIKLFISPDIEIKAGSKLIINDKEYVRSGESAIYPN
                     HQEIILELFKDKA"
     gene            complement(4228..4629)
                     /locus_tag="KRL67_001093"
     CDS             complement(4228..4629)
                     /locus_tag="KRL67_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH1521992.1"
                     /translation="MENNIIDEIEKRLESFGYILKDGDKWLIGFVREKIENIIKLDCN
                     IKTMPIELKEIEVDMIVGEFLFTKKNMGQLDIESINFEAVEKSISEGDTKVDFAIGSG
                     SQTPEQRFDSLIAYLTTYGKNKILTFRCLRW"
     gene            complement(4639..4872)
                     /locus_tag="KRL67_001094"
     CDS             complement(4639..4872)
                     /locus_tag="KRL67_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH1521993.1"
                     /translation="MAQVRKLNRILTIEECKIDDFLEMGYDLIDETGKAVKYGKSLNV
                     KDLIAENNILRSKVESLEEGNKQLKEKNKLTKK"
     gene            complement(4872..5771)
                     /locus_tag="KRL67_001095"
     CDS             complement(4872..5771)
                     /locus_tag="KRL67_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsid protein"
                     /protein_id="HBH1521994.1"
                     /translation="MAALNYAKEYSNVLAQAYPYTLNFGDLYATPNNGRYRWTGSKTI
                     EIPTISTTGRVDSNRDTIAVAQRNYDNAWEPKVLTNQRKWSTLVHPADINQTNYVASI
                     GNITKVYNEEQKFPEMDAYCISKIYADWTALGNTADTTVLTTTNVLEVFDKLMEKMTE
                     ARVPENGRILYVTPVVNTLIKNAKEIQRTVNIKDAGTSLNRQTTDIDTVKIIKVPSNL
                     MKTAYDFTTGWKVGAGAKQIFMSLVHPSAIITPVSYQFSKLDEPTAVTEGKYFYFEES
                     FEDVFILNKKADAIQFVVEGAGA"
     gene            complement(5783..6346)
                     /locus_tag="KRL67_001096"
     CDS             complement(5783..6346)
                     /locus_tag="KRL67_001096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage scaffolding protein"
                     /protein_id="HBH1521995.1"
                     /translation="MDWLEELLEGIKIENNKIDVVSLQKSIEKKIKETTITQEDYTNL
                     ETQLNTANEAIKKFEGGMTKEDVENLKTTYETDKKTLEETYKKEIEEKDFNYWLNDAF
                     KSIKCRDEIALKAHLDIEALRNSKDRQKAFEEQINPLKQDKDYLFNATLEGEEPKIDT
                     ITPGQEPKINDFGFNFTGVRPHENNNK"
     gene            complement(6656..6898)
                     /locus_tag="KRL67_001097"
     CDS             complement(6656..6898)
                     /locus_tag="KRL67_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH1521996.1"
                     /translation="MIVLMNLLEKNNEYVIYSYGYEENKLDGRIKIYLDDFYNYEIIK
                     ESKDEHISKSATLKAISKLIKAAKNNDLKKEMSYQC"
     gene            complement(6864..8357)
                     /locus_tag="KRL67_001098"
     CDS             complement(6864..8357)
                     /locus_tag="KRL67_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="minor capsid protein"
                     /protein_id="HBH1521997.1"
                     /translation="MKHKDYWRKRFEQLEEAQNNKSIKYYLELEKQYKLAMSNIERDI
                     LIWYNRFTENEGISLLEAKKLLNTRELEEFKWSVEEYIKYGKENAINQKWMKELENAS
                     ARVHITRLEALKLQIQQQVEVLYGNELDGIDKLMRDIYTSGYYHTAFNVQQGVNVGWS
                     LMSLDTNRINKVISKPWTSDGLNFSERIWGKHRPALVNELHTKLTQSIIRGENPKKLV
                     NDFAKRFKVSKSQAKNLIMTESAFFASASRKDCFNDLDVEKYEIIATLDLRTSNICRE
                     LDGKIFDMKDYQVGITAPPFHCRCRTTTAPWFEDEEGYRAARGEDGKTYYVPSSMKYN
                     EWYEKYVKNNSKQTGAKYTKGDIEWNIRREEEAELYYDNIRNRKDDISKISKNTNWSE
                     KSIGQIKNHIFYNTHIMRDGTRRMLDSDYSMSVAWQRLINGTYEDIDILLLKHEYLES
                     IFEKKYNISNLEAHRMTEKKHDWYKELIKQKGEFEEDDCLNELIRKE"
     gene            complement(8347..9786)
                     /locus_tag="KRL67_001099"
     CDS             complement(8347..9786)
                     /locus_tag="KRL67_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015984839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HBH1521998.1"
                     /translation="MLNIYISETDLIKVQLKKESTFNLVKVIEHYILKHRPEKYKQGE
                     EYYYGNTDVNNKRRYYLLDGAKVDDFTKVNNKAINNYHKLLVDQKVGYSVGNPIVFNA
                     DDDNLTKLLNDLLGEEFDDTITELYLNASNKGVEWLHPYINRKGEFKYVIIPAEEAIP
                     IWDSKRQRELVAFIRFYYIEDIDGNKIKRVEYYTENDVTYFVERGNSFVQEFLYDEYG
                     KMTDIQEGHFRINNKEQGWGKVPFIPFKNNEKCVSDLTFYKSLIDIYDNNISTLADNL
                     DEIQEVIYVLKEYPGTSLQEFIDNIRYYKSIKVDGGGGVDKLEINIPVEAKKELLDRL
                     EKNIIIFGQGVNPESQNTGDKSGVALKFLYSLLDLKCSKTEKKFKKAIRELLWFVCEY
                     LKISGSKSYDYKTVQITFNHSMIINEAEKIDMAAKSTGIVSDETIVSNHPWVEDVNDE
                     LERLKKQEDTQKEYDDLIPNNQDGVIDET"
     gene            complement(9792..11045)
                     /locus_tag="KRL67_001100"
     CDS             complement(9792..11045)
                     /locus_tag="KRL67_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBSX family phage terminase large subunit"
                     /protein_id="HBH1521999.1"
                     /translation="MVIEMIVRVNFNPDFKEANFTKKRYRAMKGSAGSGKSVNVAQDY
                     ILKLGDKKYQGANLLVVRKSEATHKYSTYAELTGAINRIYGKQADKYWKTTLNPLEIK
                     SKVTGNSIIFRGVNDAKQREKLKSINFSKGKLTWVWCEEATELMESDIDILDDRLRGI
                     LTNPNLYYQMTFTFNPVSATHWIKRKYFDYKNDDIFTHHSTYLQNRFIDEAYYRRMQM
                     RKEQDPEGYKVYGLGEWGETGGAILKNYVIHEFPTEFEYFDNMRLSQDFGFNHANVVL
                     RIGFKDGELYICNEIYVHEMDTSEIIKIANSRGLEKTLFMYCDSAEPDRIKMWKSAGY
                     KAKGVKKGPGSVKAQIDYLKQLRIHVHPSCTNTIKEIQQWKWKQDERTGLYLDEPVEF
                     MDDAMAALRYSIDNKLKNNGISFLK"
     gene            complement(11023..11715)
                     /locus_tag="KRL67_001101"
     CDS             complement(11023..11715)
                     /locus_tag="KRL67_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH1522000.1"
                     /translation="MQDVKEKVKQDYLKGMKQKEISSKYDISLNTLKSWIKRYNWASE
                     KKKGAPINKRGAPFSNKNSVGHGAPKENKNAEKFGFFSKYLPEETRELIQEISIKDKF
                     DILWEQITIQYAAIIRAQKIMYVKGKEEMVKELKKYESTENGEKIEYEFQFAWDRQAS
                     FLNAQSRAMSELRSLIKQYDEMIHKDWNLATEEQKNRVEKLKCEVDNLSKDDIGDDEL
                     KISVDYGDRNDS"
     gene            complement(11786..12295)
                     /locus_tag="KRL67_001102"
     CDS             complement(11786..12295)
                     /locus_tag="KRL67_001102"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH1522001.1"
                     /translation="MYKKVLNILLNYYKAINSKEVVELVLTPVLICIISYMFFNSSIN
                     KEVVISLNKDVLTLSGLLVAFGVCIITLLFTTYNKSISEAKDIKTERKVNGFNISYFQ
                     FIQLKAYYTVIMEILVVVICFINTIMLTRYYSNIIFYVLIFFTVHIILSLTTLIISMF
                     HLSWKDRGD"
     gene            complement(12316..13290)
                     /locus_tag="KRL67_001103"
     CDS             complement(12316..13290)
                     /locus_tag="KRL67_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH1522002.1"
                     /translation="MSNFKKRYIDVYRIDIINKINGSVYSDEEILKEVIKTFFKDKEY
                     CKENKNGINITKDKKFWITEVKCSEDDLIIKVKLEYTKYNQNTNIINAHTKEVEGNKK
                     MDQGDSNKQHLFIKFMKENNIAVVLFERVFVGVPMIELSKNLHLYYEDMIENNKFDNN
                     MSKTSINITQIASKDFIEQILDLDGVSKITLNVDREKFGTDEDNLFSNLNNSRRHNDL
                     IYKPTFRTRYLPSEVKRCCEKYIKGEEFRNKKINRIIIEGEKNKRKLKLDTEGIKLCR
                     EIEVSLDIDNHIDSEDIFDKMNNLIDDLIENNSVFFNLMYQEVAMSEA"
     gene            complement(13593..13829)
                     /locus_tag="KRL67_001104"
     CDS             complement(13593..13829)
                     /locus_tag="KRL67_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH1522003.1"
                     /translation="MPNFGTTFVTEEAVYNMSFRSNKAEAKLFTKWVTKALKQIRIHG
                     YYIATEKDQEWLDIRTEGKKVRKDFTDEIQEFVY"
     gene            complement(14314..14793)
                     /locus_tag="KRL67_001105"
     CDS             complement(14314..14793)
                     /locus_tag="KRL67_001105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 region 4 domain-containing protein"
                     /protein_id="HBH1522004.1"
                     /translation="MDKESIKEIVRELRKEEREDRKKGVLYNTRLLMKHYNDFKRHID
                     SAVSEAKDVDYLEDDLGKLDNEELYILSIKKSKARTIIMIAHIDSAMKTLKLRQERLH
                     SYEKYRALELYYCDEKTYEEIAEILNCGVVTSRRWINEMIKELGVYLFGIDGLKLTV"
     gene            complement(14786..14923)
                     /locus_tag="KRL67_001106"
     CDS             complement(14786..14923)
                     /locus_tag="KRL67_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH1522005.1"
                     /translation="MELISPGKIVRCAWCGRRFYKLDKSKVKYCSRSCSKRARRSDLS
                     G"
     gene            complement(14947..16317)
                     /locus_tag="KRL67_001107"
     CDS             complement(14947..16317)
                     /locus_tag="KRL67_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HBH1522006.1"
                     /translation="MEFKPHPYQEYTIRKTIDNNNIGLLLDMGLGKTVCALTAISELM
                     YDYFDISKVLVIAPLRVARDTWSSEVKKWEHLKHLKVSKVLGSKLDRVRALSTDSDIY
                     IINREMVPWIVDFYKRKWPFDMVVIDELSSFKSNKAQRFKSLKKVLPLTKRVVGLTGT
                     PTPNSLIDLWAQMYLLDRGERLGKTITGYKERYFEPGQKNYQTGAIYNWQPKDGAENA
                     IHNKIKDICISLKAEDYLNMPKKIDNKIEIHLDSKILKYYKELEKEKILELEKDIITA
                     SSAAVAANKLLQLANGAIYDNDKNVKELHREKLEALKEIIDVSNGKPIIVFYNYKHDY
                     NRLMKEFKSLKPRTIENSKDIYDWNNGRIQLLLCHPASTGHGLNLQSGGSIIVWFGLT
                     WSLELYQQANARLYRQGQRETVIIHHLICKGTIDEQVMEALENKDKGQSALLEAVKAK
                     LKEYRE"
     gene            complement(16266..16592)
                     /locus_tag="KRL67_001108"
     CDS             complement(16266..16592)
                     /locus_tag="KRL67_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VRR-NUC domain-containing protein"
                     /protein_id="HBH1522007.1"
                     /translation="MLESKIEKRLKKEIELLGGKAMKFISPGEAGVPDRIVLLPEGHV
                     IFVELKAPGKKLRKLQQYKMRELRELGFKVKCVSTLKEIDDFIKEVKGWNLNHIHTKN
                     TQLEKL"
     gene            complement(16874..19291)
                     /locus_tag="KRL67_001109"
     CDS             complement(16874..19291)
                     /locus_tag="KRL67_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH1522008.1"
                     /translation="MDIKASEIEHINVRHDGQLMLAIGKNKLETHWKNKSILWSELVN
                     RLSKTLRTQETYTEYRKMSKTEKDRVKDVGGFVGGSLKNGRRKAENIANRSIITLDID
                     YANKDIWEDITLLNDYACLMYSTHSHTENNPRYRLVIPLTRPVLPEEYQAISRMIADT
                     IGIDMFDDTTYQPHRLMYFPSTSIDGDYIFKFQDGEFLNPNEILDLYLDWTDVSYWPE
                     SSRERQKFNTQLKKQQDPIEKAGIIGAFCRSYSIKETIETFLNEVYIPGIDETRYTYS
                     EGSTSGGVVIYDDKFSYSHHGTDPASGILCNAFDLVRIHKFGELDEDAKPETPVNRLP
                     SFTRMSEFASSDTKVRKTIGRENLDKAKDDFGDIDFEDDEWLTRLDYDNKGSYKKTTN
                     NILMFIENDPYLKGKIAYNEFSNRAVVLGKLPWRKDDKLNDWNDSDDSGLRHHIETIY
                     NISSPSKVNDALIIAFENNTFHPIKDYLNSLKWDGIKRVDTLLIDYLGAEDNHYTRTI
                     IRKVLVAAVARVFNPGIKFDNMMVLSGPQGMGKSTFIKKLGGDWYSDSLTTVQGKEAY
                     EQLQGVWLLEMGEMMATKKADIEAVKHFLSKSEDIYRVAYGKRTSRFLRQCVVIGTTN
                     DKEFLRDKTGNRRFWPIDAGVKKTNKSIFNGQLDNERNQIWAEAVELYKANEQLYLSD
                     EEKKEAERQQRTHSEENAKSGIIEEYLNKPITKNWYDLSISEKREYIHGSDFGDLKEG
                     TILREKTCVMEIWVELFNGEPKQLTPILSREINDILKGLDDWISYNGNLRFGKIYGRQ
                     RAYVRKS"
     gene            complement(19303..21261)
                     /locus_tag="KRL67_001110"
     CDS             complement(19303..21261)
                     /locus_tag="KRL67_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase"
                     /protein_id="HBH1522009.1"
                     /translation="MRTLSIDIETYSDLDIKKVGVYRYVDSANFEILLFAYAFDNEEV
                     KVIDLVNDEELPKEVIEALNDNKVIKSAFNANFERTSISKFLNINLKPNEWSCTMIKA
                     LTLGLPGSLDSVSKALKFNEDKQKMKEGKALIQYFCKPCKATKVNKGRTRNLPIHDME
                     KWNKFKEYCKQDVVVEREIRNKLSKYKTTEREIKLWYLDQRINDTGIKVDTELIENAI
                     ECDKRYTEKLTKEAIKITGLNNPNSPAQLKKWLSDKVGFEITSLTKESIPEILKQVDD
                     ENVVRILELRKLMSKTSIKKYEAMKLAKGNDNRVRGLLQFYGANRTGRWAGRLVQVQN
                     LPQNHIEDLDLARNLLKEGDFDLIELLYDSVPDVLSQLIRTAFIPSEGHRFIVSDFSA
                     IEARVIAWLAGEKWRLDVFNSHGKIYEASASQMFKIPIENIKKGSELRQKGKLAELSN
                     GYGGSVGALISMGAIKMGLKEEELQPIVTAWRNANPNITKFWWDVDKAAKKAIKDRTI
                     VEIQHGIKFIYNPGVLFIELPSSRRLSYLRPKIEPHTTFSGDKITYEGMEQTSKQWKR
                     IDTYGPKLVENIVQATARDCLREAMFNVTDAGYSIVMHVHDELVIDVDKKGGSLEEVN
                     SIMGKEISWAKGLPLKADGYECDYYKKD"
     gene            complement(21261..21833)
                     /locus_tag="KRL67_001111"
     CDS             complement(21261..21833)
                     /locus_tag="KRL67_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2815 family protein"
                     /protein_id="HBH1522010.1"
                     /translation="MSNSVQSTKVVTGKVRLSYCNIFKSRAMVEGAEPKYSVCILIPK
                     SDKVTLGRIKKAIDAAKEQGKTSKWGGKLPGNLKTPLRDGDAERADEAEEYVGMYFLN
                     ANSTQKPGIVDKDLNEILDNTEVYSGCYGRVSINFFPYNSAGNKGIGCGLQNVQKLAD
                     GEVLGGARASAEADFSDDFEYEDEEEDFLS"
     gene            complement(21856..22428)
                     /locus_tag="KRL67_001112"
     CDS             complement(21856..22428)
                     /locus_tag="KRL67_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase subunit sigma-70"
                     /protein_id="HBH1522011.1"
                     /translation="MSNQEITKLVENNLNLVHFSINRFFKSYVEQHPYLYEEFYQEGC
                     IGLYKAALNYDSNRGKFSTVAVSYIRIEMFKALRYGEKHYRNSIEGLDVSVFEDSKRP
                     LKDIHGFYEIQFNYDVDYIRKIARKSYLKDIDKIVDWILQDKKISDIGELLNVSSTVI
                     CGRIKLFSKQVRDLDKFGEITSNIKSIYQI"
     gene            complement(22481..22816)
                     /locus_tag="KRL67_001113"
     CDS             complement(22481..22816)
                     /locus_tag="KRL67_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH1522012.1"
                     /translation="MPLDAGIKDKLDESISIILDLKDRKKSIEKDLDYEKDNLLDMLK
                     ILNITEYNSTEENAKVIVMDFKRESLIKDKVMSTFLEVNDNHLDRVYIPEHIKVSPVC
                     FVSVRAKDN"
     gene            complement(22831..23997)
                     /locus_tag="KRL67_001114"
     CDS             complement(22831..23997)
                     /locus_tag="KRL67_001114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2800 domain-containing protein"
                     /protein_id="HBH1522013.1"
                     /translation="MPLQHARLSASGAHRWLHCTPSIKLEENYPPSTSIYAEEGTVAH
                     ELAEVKLMLEYEKISKKAYNARIKKIQKSEYYNSEMEDYIQSYVENVVELVNDSKAIC
                     DDVIVMLEERLDFSEWVPEGFGTGDVVVISDGILQVIDLKYGKGLEVSAIENPQLRLY
                     GLGAYNQFEMLYDIDLIKTTIIQPRLDNISSEEIEVTKLLTWADNVKKKAQMAFNGEG
                     EFVSGSHCGFCRAKNDCRKRAEDNLKLARKYDFADTFALNKYEIADILGFAKNIQDWL
                     KDVQSYALEQAEKHGVKYPGYKLVEGRSNRKYVDEQEVAKVLLNSDYDEEKIYKPRTL
                     KGISDMEKAIGKKSFAKLLSDLIIKPVGKATLVVESDKRSEINSIDSAKKDFEI"
     gene            complement(24009..24446)
                     /locus_tag="KRL67_001115"
     CDS             complement(24009..24446)
                     /locus_tag="KRL67_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH1522014.1"
                     /translation="MVEVNVNVKVKVEAPEFTNALLVVANALGGLNLGKAMQIEPINI
                     TDMKEEKKVEVKKAEKIKKVEVKEDVKEEIAEAKEEIEKNNENTTSEVKYTKEEVRTK
                     AAQVSKAGKKDKLKELFGEFGASKLSEVKEEDYSAFMNKLESL"
     gene            complement(24517..24660)
                     /locus_tag="KRL67_001116"
     CDS             complement(24517..24660)
                     /locus_tag="KRL67_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH1522015.1"
                     /translation="MLSLDTNNKNVVTLKKDGKVIADVVFKDIKTGKKISVGILNKKV
                     LVK"
     gene            complement(24647..25039)
                     /locus_tag="KRL67_001117"
     CDS             complement(24647..25039)
                     /locus_tag="KRL67_001117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBH1522016.1"
                     /translation="MDISESITKQFSDSLKSLIEIEISKQETDRVKSQTIEQKVKVLE
                     PKDIVVLIKRGYPNYLITVEEARGILKLDTAFMRRLVSTGLIKSLVRGDGRKISRYEV
                     DDFIERNQGKNLDELLKAVERGDEIAES"
     gene            complement(25070..25267)
                     /locus_tag="KRL67_001118"
     CDS             complement(25070..25267)
                     /locus_tag="KRL67_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBH1522017.1"
                     /translation="MLIGDNIKQILRKRDIKPYKLAKELDIDVSGLYKLLRNKSSNPT
                     IDTLIKLADYLDITLDELVGR"
     gene            25343..25717
                     /locus_tag="KRL67_001119"
     CDS             25343..25717
                     /locus_tag="KRL67_001119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH1522018.1"
                     /translation="MQFSNNISKELQESIRKTVSDTSNTEYFYYAEFQYKIILKSIEE
                     FEKELDDEHEIALKLTNFGKDVLMIVEEVGYHNPCLIHYYGIVNGVYSEILQHTSQIN
                     FMITSVKKTDPSKPARRIGFIL"
     gene            complement(25727..25936)
                     /locus_tag="KRL67_001120"
     CDS             complement(25727..25936)
                     /locus_tag="KRL67_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH1522019.1"
                     /translation="MENKKIEEIEKRITMLENQLQAQLFSQITAQSNSDFIDYMYQCI
                     VLYLHEMNKSEKRKISEITKERIRK"
     gene            complement(25983..26813)
                     /locus_tag="KRL67_001121"
     CDS             complement(25983..26813)
                     /locus_tag="KRL67_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor KilAC domain-containing
                     protein"
                     /protein_id="HBH1522020.1"
                     /translation="MSNNLMVFEGKEVEVFEFEGQILFNPKHVAECLDIKNVNENLRN
                     MNNKQVIKLINSKISSTDFRKLHNTGENFLTESGVYKLIFKSRKEEAERFQDWVTDEV
                     LPSIRKTGTYNMVNRQLKDSYMIDNPIERAKRWIEEQKEKEQLQLESKMKEKVIKELK
                     PKADYTDMILKNKGLVTITQIAKDYGMSGKEMNKILHERGIQYKQSGQWLLYKQHQGK
                     GYTHSETIDITRSNGMPDVKMTTKWTQKGRLFLYDLLKTNNILPDIEKEYSYQTSILG
                     "
     gene            complement(26933..27706)
                     /locus_tag="KRL67_001122"
     CDS             complement(26933..27706)
                     /locus_tag="KRL67_001122"
                     /inference="COORDINATES: protein motif:HMM:NF015340.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HBH1522021.1"
                     /translation="MKDLKIVKVNNKLTTDSRDIALMVEKEHKILLRDIRNYINQMEE
                     ANKNMSTDLYPSDYFIENTYLDDYKRKKPCYAITKIGCDFIANKMTGIKGTAFTGIYT
                     KKFDEMEKALKNEQTKLPTTYKEALQQLLIEVEEKEQLQLENQTMKPKADYFDALVER
                     NLLTNIRDTSKELGVKEKVFVLWLIEKKYCYRDLKGKIKPYSNKMQYFELKEFTTPYG
                     HSDTQTLINPKGREAFRLLLIKDGLIREKERECQITLLG"
     gene            complement(27737..27952)
                     /locus_tag="KRL67_001123"
     CDS             complement(27737..27952)
                     /locus_tag="KRL67_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBH1522022.1"
                     /translation="MYVNRLKGLMKENQHTQKFVADLLGLSLFGFRLKLNGKNEFKAN
                     EIKKLSELYGVSTDYFFSELVAKMAIK"
     gene            28133..28678
                     /locus_tag="KRL67_001124"
     CDS             28133..28678
                     /locus_tag="KRL67_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HBH1522023.1"
                     /translation="MFRLKELREEKGISLDRLSMDLNVNKSTLSRIENGLREPKQSFI
                     EDCSNYFDVSTDYLLGKTDIKNPNQVVKSLNPIFSKRLRELIKEKGVTLNILGKKLEI
                     STDLLSKYENNIISPKSDDIIKLAHYFDVTTDYLLGTTLVRNHIDTVAAHKANPHEDL
                     PEEAQEQLNDYIEFLMNKYKK"
     gene            28702..30291
                     /locus_tag="KRL67_001125"
     CDS             28702..30291
                     /locus_tag="KRL67_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIR2 family protein"
                     /protein_id="HBH1522024.1"
                     /translation="MKSIISDVINRFNATPFLFVGSGLTRRYYNLPNWEDLLKVFAEK
                     ISNDDFIYTSYKNKAKSENPKMGINPRIAELIENDFNKKWFSDSSIRSLDSKYLDIVK
                     SGVSPFKAEIAMYIKNNSKIVDKYQDEVEKLSNISKKSLSGFITTNYDCFLETIVDNY
                     TTYIGQENLVFSSIQGIAEIYKIHGCVSSPNSIVINEADYIDFDSKSAYLAAKLMTIF
                     VEFPIIFIGYSVTDVNIKKILNAIVNCLSNENAKKLEERFIFIEYEKNFNDIEISSHT
                     IAFDNKMITMTKIKLEDFNLLYEALSEKKSKLPVRILRMFKQEFYDFTITNKPTAKIR
                     VGSVDDTRIKDEDLVLAVGKASDFGLKGLKGLSFDEWYRDIVMNDLEFSSDELLEYAY
                     PSLIKQYNKLPLNKHLFNSTLEFPDYRTIALESDFENIISNSIKKNRNNTYIKNRSVY
                     GIWNDEHQSFEKATRLIAFLEEKEIDTEQLENLLKKIFEENPNILESAKTSEKTNLRR
                     LIRIYDYMKYSNMQKSLGYSE"
     gene            30554..>31055
                     /locus_tag="KRL67_001126"
     CDS             30554..>31055
                     /locus_tag="KRL67_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HBH1522025.1"
                     /translation="MNKLDALLDLANNEEIEIYYTDKIADDIKGLYINRQGLKIISLL
                     NSLKQNNAKLIEILAEELGHHFTSVGNYVSSKNSYKNKILIDKTENKALKWACEFLIT
                     EEEIIHVINSHATSVYEIAEELQVSINFLLKRLEFLSKKKSMLDLGNNRFLVLTNLPN
                     FYIYEDI"
BASE COUNT         9429 a         5756 c         3222 g        12648 t
ORIGIN      
        1 aagaactaca atctattttg cggtagagtg aagttcataa ttaaatgaat ctatttgaac
       61 ttatggaact tgattttaaa atcaaattcc cagccacttt tactcttgcc acgagttgag
      121 tggcttttta cttttagaaa tactttacaa attaagcaaa atattaaact agcaataacg
      181 ccagctatta cattaagtaa aaagttattc atacttccca cctcctttca ttaggaagta
      241 ggttttatct cagtatgaac tccactctat aaattgtaga ttacatcttc ttgctaaaaa
      301 tattataaca tataattatt acatatttta cctattctat atttattttt ttattttgct
      361 atcttcttcg tcccctcttt ctctctcttt cagcttcttt cattgcttcc tcttcatctt
      421 ctatctttat aagtattgag gcggctgcta acgctctctc attaacttct aaattcatat
      481 attcacttgg cttccacttt aatttttgaa tacaataatg agtgatgcta gcatcaaaat
      541 cgccacctct gattagtttt ttgcttcttc tactttatcc tcaaaagatg tatcaaatcc
      601 attgacttca ttcactttta ctgtataatt gacatactca cctgctgtaa gcattgtctt
      661 taataactga gcttctccca ttactccata actattttgg agttcggcat cctttaaatc
      721 tggaaatact gtagatgcta cacataattc agctacataa ctattgtagt caatttcact
      781 tgtatattgt ccagtatgct taccattgtt accaatcact tttactcttt tagtacactt
      841 tcttcttagt gcttcgtctt cttcagatga taaaactttt aattcccatt caactggttt
      901 cccttcttta tctaaaaatc tgttactcgc tacatatttt acattatcaa cctttattgc
      961 attttgactt aaaaaagcac ttaaattact catattattc taatctcctt ttattttaat
     1021 ttttcatata aaaaatacac atatataatt tataaatgtg tattttactc cattcctgcc
     1081 aataaattaa atttttctac taattcccaa tcctcaaaag tgaaatccat atcttcatct
     1141 aaatactcac catcagcatc aaatttagta attattccac tgtccatatt acaatcttta
     1201 agtactactg tctgtcttcc tacagcagat gtagggtctt catttgtaac ttgtatgtca
     1261 aaataaatat cctcaccagt ttctttatat ctgtaaagta attctctaaa aatagaagta
     1321 ttataatgaa atgttgcact tccagtattt gtactcccag ttgttttatt tccctttgtt
     1381 gttcttccta gaattggaac ttcactttta tttttttcca ttttagcctc taaatctata
     1441 gcttgcataa aattatatct tttgccttct atagttataa aacattcagc tttctttgca
     1501 ctaactgtat ctttagcatt tattgtttga gccatattat cactcccctc tctaactaac
     1561 tgaaactgtc atataaagct tactcatagc atttataacc ttaacagcat cagatactat
     1621 gacagttttc ttatcatttc caagctctac actaacatca tcagttttaa aatcttctat
     1681 tgcccttata ttctctaatt ctttatggtg tttaacaaca tcattccaga aacttattct
     1741 tcctgcctta tcattcggaa ctttacctaa atacttttca ttaaataaag ttgcaatatc
     1801 attagcaatt tggtcaagta ctctaacact ttggttactt gaaaaatcgt catttttatc
     1861 atctgtaaat gatacaaaag tatttatatc ctctaacaca tgaacttcat ctcccacctt
     1921 gtggaatata aatttaccag tttttaaagc ttcttcaagt tgtatttgtg tataattaac
     1981 atcaacatca aactcaccat catacttttt attagtatta gatttattta tatcgcatcc
     2041 tgctatagct ccagccgccc aataaattaa actagattca actaaatctt tatctttaat
     2101 cttattttct acagacacta caccttcata atctgcatca cttttcttat atagtactgt
     2161 ttgaaactta gctcctacct tatctctcat tctctttgta aattctacaa ataaactttt
     2221 aatttctgtt gttgtagcca aacatcctaa agcattaaag ctatagcttt ctattttatc
     2281 caagaaagct tggtactctg ctcctgtcac agcttcgcca ttagttccac cagtaaatac
     2341 aagtcctgca cttgcttcta gtgttgcatc cttcttccaa gtgatatagt cattgtcttg
     2401 taagtctgta ataacctttg ctatttgagt atctaccttc ttattatcta aaagtgttac
     2461 aacatcaaac ttagcattat catctatatt tgttgtaact gttactttta agtcatttcc
     2521 tctgatacca ctatattttg ctgtggctat agtacaactg gctttaacgc ctttatttaa
     2581 tttataaaaa tatcccaacc ttatattttt gaataaatct ctcaaacctt tcagcttctc
     2641 atgagtataa tcatatccaa aatacttcac tgaatacttc tcaaaatcat cactggttac
     2701 ttggaatact tcttcatcta tgccccaatc taactctaaa ggtattgcaa caatacctct
     2761 atccgataat gaactggttg cccttgtagc acttacaaaa tttatatatg caccaggtag
     2821 gaccttattt tgtgttacaa atgttcctcc acctaaagcc atctagctca ctcctttcat
     2881 aaaattattt atcatatctt ccacttctga gaaagaatac aattcatctt cctttaagat
     2941 tgcatttaat aaatcttttc tatttacata ccttttagaa ttaactattt gctccttagt
     3001 aaacttataa ttttcttctt tacttaatgt tttactcaag tttatcacct ctctttagac
     3061 caccaaacaa ctctactgtg tccatcttat ctgtattatt gctttttata gtgaaatagt
     3121 tataatcaac aaagaaatga agaacattat ctacaatttc aaagttcata tttgtacctc
     3181 tgactaaatc tccatcaatt tctatatact ctaattcctc cagtagcatc tcagctacct
     3241 catttatttc aaatgattta tcattacttt tgggaaaata atgtacatca aaagagttct
     3301 tttttaatgt cctgccactt ggatatgata ctttgcttgg atttaaagga acaataaaaa
     3361 aacaaggttc atttatacct tgctccacat cttcactata aattgtataa ctctctccaa
     3421 atgttttatc taatttaata gatattccat caattatatt attaagcatc aaatactcct
     3481 ttaagtaata ttaataactt tttctctata atcttatcaa cttggctttg tagttccatc
     3541 tctgaaattg ttaagaaatg ttgtccttta acccaacctt ttccatcttt agttctatgc
     3601 ccgaaattta catagcttgc atattcagtt ggattaacaa cttctataat ataattatta
     3661 ccttgtttgt aaacaggaag cgacctagca taagctactc cactccaacc ctctctcaaa
     3721 aatcctgtgt cgactggtgt tcttctaatt acttttccta aaagtcttgc tgctaattct
     3781 cttgctgcat ccttgcaaaa cttatctaaa tcaatctttg taagcttctc caccttttta
     3841 caaactcttt taaactctct aaaatcaaca ctgccccatc tagccattat gctttatcct
     3901 taaatagctc aagtattatt tcttgatgat ttggatatat agctgattct ccacttctta
     3961 catactcttt atcatttata ataagttttg aacctgcttt aatttctata tctggagata
     4021 taaagagttt aatagtttgc tctagcttag ctaatttccc ttctgtagca gaaactatat
     4081 ttttatatga aagtttacat ggttgatttt ctaatacaat cacttcttta ttgttagttc
     4141 gttttgttac agggtcttta attggctgat actcaactat agtacattta tctctatata
     4201 acatttctat tgcttttcta gttttactta ccatcttaag cacctaaagg ttaatatctt
     4261 attcttacca taagtagtaa gataagctat taagctatca aagcgttgtt ctggtgtttg
     4321 agaaccactt cctatagcaa aatctacctt tgtatcacct tctgatatag acttttctac
     4381 agcttcaaag ttaatgcttt ctatatctaa ttgacccata tttttcttgg taaataaaaa
     4441 ctctccaact atcatatcaa cttcaatttc tttcaattca attggcatag tttttatatt
     4501 acaatctagt ttaataatat tttctatttt ttctcttaca aaacctatta accacttatc
     4561 tccatctttt aatatatatc caaaactttc aagtcttttt tctatttcat caattatatt
     4621 attttccata attttcacct actttttagt aagtttattt ttctctttaa gctgcttatt
     4681 tccttcttct aaagactcaa cttttgacct taaaatatta ttttcagcta ttaaatcttt
     4741 tacatttaat gacttgccat actttactgc cttaccagtt tcatctatca aatcatatcc
     4801 catctctaag aaatcatcta ttttacattc ttctatagtt aatattctat ttaatttcct
     4861 tacttgtgcc attatgctcc agctccttca acaacaaatt gtattgcatc agctttttta
     4921 tttaatataa atacatcctc aaaactttct tcaaagtaga agtattttcc ctctgtaact
     4981 gctgttggtt cgtctaactt agagaactga taagaaacag gtgtaattat tgcacttggg
     5041 tgaactaagg acataaagat ttgtttagct cctgctccta ctttccatcc agttgtaaaa
     5101 tcatatgcag ttttcattag attagatggt actttaatta ttttaactgt gtcaatatca
     5161 gttgtttgac gattaagaga agttcctgca tcctttatat ttactgttct ttgtatctct
     5221 tttgcatttt tgataagtgt atttactact ggagtaacat acaatattct tccattttca
     5281 ggtactctag cttctgtcat tttttccatt aacttatcaa atacttctaa tacgtttgtt
     5341 gttgtaagaa cagttgtatc tgctgtatta cctaatgcgg tccaatcagc atatatttta
     5401 gatatacagt aagcatccat ctctggaaac ttttgttcct cattatatac ttttgttata
     5461 ttgcctattg aagccacata attagtttgg tttatatctg ctggatgaac caatgttgac
     5521 catttccttt gattagttaa taccttaggt tcccaagcat tatcatagtt tctttgagct
     5581 actgctattg tatctctgtt tgaatctact cttccagttg tagatatagt tggtatttct
     5641 attgttttag aaccagtcca tctatatctt ccattatttg gtgttgcata caaatccccg
     5701 aagtttaaag tataaggata tgcttgtgct aaaacatttg aatattcttt tgcataattt
     5761 agtgctgcca ttttatttcc tcctatttat tattattttc atgaggtctt accccagtaa
     5821 aattaaaacc aaaatcattt atcttaggct cttgccctgg tgttatagta tctattttag
     5881 gctcttcacc ttctagtgtt gcattaaaca aataatcttt atcctgtttc aaagggttta
     5941 tttgctcttc aaaagctttt tgtctatctt tactatttct tagtgcttct atatctaaat
     6001 gagcttttaa tgctatttca tccctacatt taatagactt aaaagcatca tttaaccagt
     6061 aattaaagtc cttttcttca atttcttttt tgtaggtttc ttccaaagtt ttcttatcag
     6121 tttcataagt tgtttttaga ttctctacat cttcttttgt catacctcct tcaaactttt
     6181 taatagcttc attagctgta ttaagttgtg tttcaagatt tgtataatct tcttgagtaa
     6241 ttgtagtctc ttttattttc ttttctatag atttttgaag agaaactaca tcaattttgt
     6301 tattttctat ttttattcct tctagcaatt cttctaacca atccatcttt taaatatctc
     6361 ctttcatttt ttacacaata aaaaaagtct ttaaagactt ttgtctagtg atttatttaa
     6421 ttcttctcct actctcttta gaatttcatc agaaacttta tctatattag cttcattttt
     6481 ttcttttaat ttattgcaaa gaatacctac attgacatta gcaatacaat taataaagat
     6541 ttgtactaca caaactacag ctataacttg agttaatatt tacattaaaa ttacctactt
     6601 ttttaaactt ttacataata aaagcaccta ctaatttatt gttcagtaag tgcttctaac
     6661 attgataact catttctttt ttcaaatcgt tatttttagc agcttttata agtttagaaa
     6721 tagcttttaa cgttgctgat ttacttatat gttcatcttt tgactctttt attatttcat
     6781 aattataaaa atcatctaaa tatattttta ttcttccatc aagcttattt tcttcatatc
     6841 cataactata tataacatat tcattattct tttctaataa gttcattaag acaatcatct
     6901 tcttcaaact ctcctttctg tttaattaat tctttatacc aatcatgctt tttctcagtc
     6961 attctatggg cttctaagtt acttatatta tactttttct caaatatact ttcaaggtat
     7021 tcatgtttta ataagagaat atcaatatct tcgtatgtac catttataag tctttgccaa
     7081 gcaactgaca tactatagtc agaatccaac atacgtctag ttccatctct cattatatga
     7141 gtattgtaga aaatatgatt tttaatttga cctatacttt tttctgacca atttgtattc
     7201 tttgatattt tggaaatatc atcttttcta tttctaatat tatcgtaata tagttctgct
     7261 tcttcttctc ttcttatatt ccactcgata tcacctttag tatattttgc accagtttgt
     7321 ttactattat ttttaacata cttctcatac cactcattat acttcatact agatggtaca
     7381 taatatgttt ttccatcttc tcctcttgct gctctatagc cttcttcatc ctcgaaccaa
     7441 ggagctgttg ttgtcctaca acgacaatga aatggtggag ctgttattcc aacttgataa
     7501 tctttcatat caaatatttt tccatctaac tctctgcata tatttgaagt tcttaaatct
     7561 aatgtagcaa taatctcata tttctctaca tctaaatcat taaaacaatc ttttcttgaa
     7621 gctgatgcaa agaaagctga ttcagtcatt attaagttct tagcttgtga cttagatacc
     7681 ttaaatctct tagcaaagtc atttactagc ttctttggat tttcacctct aataattgat
     7741 tgagttagct tagtatgtaa ttcatttact aaagcaggtc tatgcttacc ccaaatcctt
     7801 tcactgaagt ttaatccatc actagtccaa ggcttagaaa taactttatt tattctgtta
     7861 gtatcaagac tcattaaact ccaaccaacg tttactcctt gttgaacatt aaaagctgta
     7921 tgatagtatc cacttgtata aatatctctc attagtttat caataccatc aagttcattt
     7981 ccatataaaa cttctacttg ttgctgtatt tgcagtttta aagcttcaag ccttgttata
     8041 tgaactcttg cactagcatt ttctaactct ttcatccact tttgatttat agcattttct
     8101 ttaccatatt taatatattc ttctacactc catttaaact cttctagttc tcttgtattt
     8161 agcagtttct tagcttccaa taaagatatt ccctcatttt cagtgaatct gttataccat
     8221 ataagtatat ctctttctat attagacatt gctagtttat attgtttttc taattcaaga
     8281 taatatttta tacttttatt attctgagct tcttctaatt gttcaaatct cttcctccaa
     8341 taatctttat gtttcatcta taacaccatc ttgattatta ggaattaaat catcatactc
     8401 tttttgagta tcttcctgtt ttttaagtct ttcaagttcg tcattaacat cctcgaccca
     8461 aggatggtta gaaacaatag tttcatctga tacaattcca gttgatttag ctgccatatc
     8521 tatcttttca gcttcattta ttatcataga gtgattaaaa gtaatttgaa ctgttttata
     8581 atcatagctc ttactaccac ttatctttaa atactcacac acaaaccata aaagctctct
     8641 aattgctttt ttaaacttct tttcagtctt agaacatttt aagtcaagta gtgaatataa
     8701 aaatttaagt gctacacccg atttgtcacc tgtgttttga gattctggat taactccttg
     8761 accaaagata attatattct tttccaatct atcaagaagc tcctttttag cttcaactgg
     8821 tatatttatc tctagtttat caactccacc tccaccatct actttaattg atttatagta
     8881 tcttatatta tctataaact cttgtagact tgttcctgga tattctttta atacataaat
     8941 aacctcttgt atttcatcta agttatctgc tagtgtagaa atattattgt catatatatc
     9001 tattaatgat ttatagaaag ttaaatctga gacacacttt tcattatttt taaaaggtat
     9061 aaatggaact ttaccccaac cctgttcttt gttatttatt ctaaaatgac cttcttgtat
     9121 atcagtcatt tttccatatt catcatataa aaattcttga acaaaactat tacctctttc
     9181 aacaaagtaa gttacatcat tttctgtgta gtactcaact ctttttattt tatttccatc
     9241 tatatcttca atataataaa acctaataaa tgcaactaat tccctctgtc ttttactatc
     9301 ccaaatagga attgcttctt cagctggaat tattacatat ttaaactcac cttttctatt
     9361 aatatatgga tgtaaccatt caaccccttt attactagca ttgagatata gttctgttat
     9421 tgtatcgtca aactcttctc ctagtaagtc atttaaaagc ttagtgagat tatcatcatc
     9481 tgcattaaat acgattggat ttccaacact atagcccact ttttggtcaa ctaaaagctt
     9541 atggtagttg ttaattgctt tattattaac cttagtaaaa tcatcaacct tagctccatc
     9601 taagagataa tatcttctct tattgtttac atcagtatta ccataatagt attcttctcc
     9661 ttgtttatat ttttctggcc tatgctttaa gatgtagtgt tctatgactt ttactaggtt
     9721 aaaggtgctc tcttttttta actgaacttt tattaaatct gtttcactta tataaatatt
     9781 taacacctcc tttactttaa gaagcttatt ccattatttt taagcttatt atctatagaa
     9841 tatctaagcg cagccattgc atcatccata aactcaactg gttcatcaag atataatcca
     9901 gttctttcat cttgtttcca tttccattgt tgtatttctt ttatggtatt agtgcagcta
     9961 ggatgtacat gtattcttaa ttgtttcaaa taatctattt gagctttaac acttcctggt
    10021 ccttttttaa ctcctttagc tttatatcct gcactcttcc acatcttaat tctatctggt
    10081 tcagcactat cacagtacat aaatagagtc ttttctaaac ctctactatt tgcaatcttt
    10141 atgatttctg aagtatccat ttcatgtaca tatatttcat tacatatata taattctcca
    10201 tccttaaagc caattctaag tactacattt gcatggttaa atccaaagtc ttgtgataac
    10261 ctcatattgt caaaatactc aaattctgta ggaaattcat gtataacata atttttaagt
    10321 attgctccac cagtttctcc ccattctcca agaccataga ctttgtaccc ttctgggtct
    10381 tgctcttttc tcatttgcat tcttctgtag taagcttcat ctatgaatct attttgtaga
    10441 taagtactat gatgagtaaa tatatcatca tttttatagt caaaatactt tctttttatc
    10501 caatgagtag ctgagactgg attaaatgta aatgtcattt gataatacaa gttaggatta
    10561 gttaaaatac ctcttaaacg gtcatctagt atgtctatgt cactttccat aagttctgta
    10621 gcttcttcac accaaaccca tgttaatttt cctttcgaga agttaattga ttttaatttt
    10681 tctctttgtt ttgcatcatt aactcctctg aaaattatag agttaccagt aactttactc
    10741 ttaatttcta aaggatttaa agtagttttc caatacttat cagcttgttt accataaata
    10801 cgatttatag ctcctgtaag ctctgcatac gttgaatact tatgtgtagc ttctgacttt
    10861 ctaactacta atagattagc tccttgatac ttcttatctc ctaactttag tatatagtct
    10921 tgtgctacat taacagattt tccactccct gctgaacctt tcattgctct gtatcttttt
    10981 ttagtaaaat tagcttcctt gaaatctgga ttaaaattta ctctaactat catttctatc
    11041 accataatct acacttattt tcaactcatc atctccaata tcatctttac ttaggttatc
    11101 aacttcacat ttcaacttct caactctatt tttctgctcc tctgtagcca aattccaatc
    11161 cttatgaatc atttcatcat actgtttaat taaacttcta agttcactca tagccctact
    11221 ctgtgcatta agaaaagatg cttgcctatc ccatgcaaat tgaaattcat actctatctt
    11281 ctcaccattt tctgtgcttt catatttctt taattcttta accatttctt ccttgccttt
    11341 aacatacatt atcttctgtg ctcttattat tgctgcgtat tgaattgtta tctgttccca
    11401 aagaatatca aatttatctt ttatagatat ttcttgtatc aattccctag tttcttcggg
    11461 tagatatttt gagaagaaac caaacttttc agcgttctta ttctcttttg gagcaccatg
    11521 accaactgaa tttttattag aaaagggtgc acctctttta tttataggtg cacccttctt
    11581 tttttcacta gcccagttgt atctttttat ccatgacttt aaagtgttta agctaatgtc
    11641 atactttgat gatatttcct tttgtttcat tccttttaag taatcttgtt ttaccttttc
    11701 tttgacatct tgcacatcac cacctcttta tttgtttgtt ttgggaataa aaaaaacaat
    11761 cctatttaat aggattgttt caaatctagt ctcctctatc cttccaggat aaatgaaaca
    11821 tacttattat taatgtagtt agagataaaa ttatatgaac tgtaaaaaat attaaaacat
    11881 agaaaattat attagaataa tatcgagtaa gcattattgt attgataaaa catattacaa
    11941 caaccaaaat ctccattatt acagtataat atgcttttaa ctgtatgaat tgaaagtatg
    12001 atatattaaa tccatttacc tttctttctg tttttatatc ttttgcttct gatattgatt
    12061 tgttatatgt agtaaacaat aatgtaataa tgcaaactcc aaaagctact aatagtcctg
    12121 acaaagttag tacatctttg tttaaagaaa ttacaacttc tttattaatt gacgaattaa
    12181 aaaacatgta tgaaataata catattaata ctggtgttaa aactaactct actacttctt
    12241 tagaatttat agccttgtaa taattgagta agatatttaa taccttttta tacattttaa
    12301 cacctccttc gcaatctatg cttcactcat agcaacttct tgatacatta aattaaaaaa
    12361 aactgaatta ttctcaatta aatcatctat caaattattc atcttatcaa aaatgtcttc
    12421 actatctata tgattatcta tatcaagaga aacctctatt tctctacaca actttatacc
    12481 ctcagtatcc aactttaatt ttctcttgtt tttctctccc tcaattatta tcctatttat
    12541 ttttttattt ctaaattcct ctcctttaat atatttttcg caacatcttt taacttcact
    12601 aggtaaatat ctagttctaa atgtgggctt atatatcaaa tcgttatgtc gtcttgaatt
    12661 atttaaatta gaaaacaaat tatcttcatc agttccaaac ttctctctat caacatttaa
    12721 agttattttt gaaacaccat ctaaatctaa tatttgttct ataaaatctt tacttgctat
    12781 ttgtgtaata ttaattgatg tttttgacat attattatca aatttattat tttcaatcat
    12841 atcctcataa tatagatgta agtttttact tagttcaatc atgggaaccc ccacaaaaac
    12901 tctttcaaat aatactactg ctatattatt ttccttcata aatttaataa ataggtgctg
    12961 cttattgcta tctccttgat ccattttttt attaccctct acttctttag tatgagcatt
    13021 tataatattt gtattctgat tgtatttagt atattcaagt ttcaccttga ttattaaatc
    13081 atcctcgcta cattttactt ctgtaatcca aaacttttta tctttagtaa tattaattcc
    13141 atttttgttt tctttacaat attctttatc cttaaagaag gtctttatta cctcttttaa
    13201 aatttcttca tctgaataga cactcccatt aattttattt ataatatcta ttctataaac
    13261 atcaatatat ctttttttaa aattactcat tataccatta ttcctttcac aaatatttat
    13321 aatgatataa ttctatataa tcagtcaata tccttcaata atcattcgac aaccacaaaa
    13381 taagtctaaa taatactttt accttattcc tgtctataat gtacaaatat atttcatttt
    13441 taattcatta cataaaaaaa agaccctcca ttaatacagt catttaaatc atttctatta
    13501 attctttaat cttttatata cctctttata attcatactt ttatctatca acctaggtga
    13561 tttcatagat ataactcttt caagtgcttg tattaataaa caaactcttg tatttcatct
    13621 gtaaaatctt ttctgacctt tttaccttct gttcttatat ccagccattc ttgatctttt
    13681 tctgtggcaa tatagtaacc atgtattcta atttgtttaa gtgcttttgt aacccacttc
    13741 gtaaatagtt ttgcttctgc tttattactt ctaaatgaca tattgtacac agcttcttct
    13801 gtaacaaaag tagtaccaaa gttaggcaat ttatctttaa agtttctagt gtaggaatct
    13861 ccgacagtag actcattaat ttctttttat attctctatc tatatttctt aatgtatcac
    13921 gaatatttac tatgcctagt tcctctccta cgtcatttgc attaaatcaa acttcttctc
    13981 cattttttga ccatatcatc cttacatttt tctcttgtaa aattttcaac atactactac
    14041 ctcctaaatt agttattaat aaagaatatt caatttgaac acccttattt tcgacccttc
    14101 aatatgaggg attaaaattg tataaaaaag acctagaaat cactctaagt catgaattac
    14161 tcatttatca ttctccttat ttacaaaata aaaaagacta agttggggta acttagcctt
    14221 tttcaagggg gaatatatta tacacttgtt tcatactacc attataacag ggttaaaata
    14281 tcattaaaat atcatctttt tatcatatat ttatcaaaca gttaatttca agccatctat
    14341 accgaataaa tacactccca gttcctttat catttcatta atccaacgtc ttgatgtaac
    14401 tactccacaa tttaatatct ctgctatttc ttcatatgtt ttttcatcac aatagtaaag
    14461 ctctaaagct ctatactttt cataagaatg taaacgctct tgtcttaatt tcaaagtttt
    14521 cattgctgaa tctatatgtg ctatcataat aatagttctt gctttacttt tctttatact
    14581 taaaatatac aactcttcat tatcaagctt acctaaatca tcctctaagt aatctacatc
    14641 tttagcttca cttaccgctg agtctatatg tctcttaaaa tcattataat gcttcatcaa
    14701 taatctagta ttatataaaa cgcctttttt cctatcttct ctttcttctt ttcttaactc
    14761 tcttacaatt tcttttatac tttctttatc cacttaaatc actccttctt gctctcttcg
    14821 aacaacttct actgcaatac ttaactttag atttatccaa cttataaaat cttctaccac
    14881 accaagcaca tcttactatt ttacctgggc ttataagttc catcttttct ttttactttc
    14941 ataccttcac tccctatatt cttttaattt tgctttgact gcttcaagta atgcactttg
    15001 tcctttatct ttattctcta gagcctccat tacttgttca tctattgtac ctttacagat
    15061 tagatgatgg ataataacag tttccctttg cccctgtcta taaagccttg cattggcttg
    15121 ttgatacagt tctaagctcc aagttaatcc aaaccaaaca attatacttc cacctgattg
    15181 taaattaagt ccatgtcctg tacttgctgg atgacaaagt aataattgta ttcttccatt
    15241 attccaatca tatatatctt ttgagttttc tattgttctt ggtttcaaac tcttaaactc
    15301 tttcattaaa cgattataat catgtttata gttataaaaa actataatag gcttaccatt
    15361 tgaaacatct ataatttctt ttaaagcttc taatttttct ctatggagtt cctttacatt
    15421 tttatcatta tcataaattg ctccattagc taattgtaaa agtttatttg ctgctacagc
    15481 tgctgatgaa gctgttatta tatctttttc taattctaat atcttctctt tttctaattc
    15541 tttgtaatat ttaagtattt tactatcaag gtgtatttca attttattat ctattttttt
    15601 aggcatattc aaataatctt ctgcttttaa gcttatacaa atatctttta ttttattatg
    15661 tattgcattt tctgctccat ctttaggctg ccaattatat atagctcctg tttgataatt
    15721 tttttgtcct ggctcaaagt atcgttcttt atatcctgta atagttttac ctagtctttc
    15781 acctctatca agtaagtaca tctgtgccca taagtctatt aaactatttg gtgttggtgt
    15841 accagtaagc ccaactactc ttttagttaa aggcaatact ttctttaaac ttttaaatcg
    15901 ttgtgcttta tttgatttaa aagaactaag ttcatctatc acaaccatat caaatggcca
    15961 ttttctttta taaaaatcca ctatccaggg taccatttct ctatttatta tataaatatc
    16021 tgagtctgta cttaaagctc tcactctatc taatttacta ccaagaactt tagatacttt
    16081 tagatgcttt agatgctccc atttttttac ttcactactc caggtatctc ttgcaactct
    16141 taaaggtgct ataactaaaa cttttgatat atcaaagtaa tcatacatta attcacttat
    16201 agctgttaga gcacagactg ttttacctaa tcccatatct aatagtaaac ctatattatt
    16261 attatctata gtttttctaa ttgtgtattc ttggtatgga tgtggtttaa attccatccc
    16321 ttaacctcct tgataaaatc atctatctct tttaatgtgc ttacacactt aactttaaat
    16381 cctaattctc ttaattctcg cattttatat tgttgaagct ttcttagttt tttacctggt
    16441 gctttaagtt ctacaaatat aacatgtcct tctggtaata agacaatcct atctggcaca
    16501 cctgcctcac ctggtgaaat aaacttcata gcctttccac ctaacaactc aatctctttt
    16561 ttaagtcttt tttctatttt tgattctaac aaaatatcac ttccttaaaa tttaagtgtc
    16621 aacaatgtca acagattttc tatatatatg tatatataca tattaggcat acatatgtat
    16681 atacgtatat gcctaatatt acttatacta ctttatactt aatttttgtt gacattgttg
    16741 acattaatac ctcaaacatt gtaattactg catcttaggt gtcaacataa gcgtcaacaa
    16801 agccattttt tttgttgaca ttgttgacac ttttaaaatt ttcaaattta ctctattttt
    16861 aattctgttg acactaactt ttacgtacat aagctctctg cctaccatat attttcccaa
    16921 accttaaatt tccattatat gatatccaat catctaatcc ttttaatata tcattaattt
    16981 ctcttgataa gataggcgtg agttgtttag gttctccatt aaatagctca acccatatct
    17041 ccataacaca cgttttttct cttaatattg tcccttcttt taaatcacca aaatctgaac
    17101 catgaatata ttctctcttt tctgaaatac ttaaatcata ccaattctta gtaataggtt
    17161 tatttagata ctcttcgata attccagatt tagcattttc ttctgagtga gttctttgtt
    17221 gtctttcagc ttcttttttc tcctcatctg atagatatag ctgttcattg gctttatata
    17281 attctactgc ttctgcccaa atttgatttc tttcattatc aagttggcca ttaaatatac
    17341 tcttgttagt cttctttact cctgcatcta ttggccaaaa ccttctattc ccagtcttat
    17401 ctcttaaaaa ttctttatca ttagttgttc ctataactac acattgacgt aaaaatcttg
    17461 aggttctctt cccatatgcg accctatata tatcctctga tttgcttaga aaatgcttaa
    17521 ctgcctcaat atctgctttt ttagtagcca tcatttcacc catttccagc aaccatactc
    17581 cttgtaattg ttcatacgct tctttacctt gtacagtagt taaactatca gaataccagt
    17641 ctccaccaag ttttttaata aaagtacttt ttcccattcc ttgaggacca gataaaacca
    17701 tcatattatc aaactttatt cctggattaa atactcttgc tactgctgct actaaaactt
    17761 ttcttattat agtccttgta tagtggttat cttctgcacc taaatagtca attaaaagtg
    17821 tatccactct cttaatgcca tcccacttta aagaatttaa ataatctttt ataggatgaa
    17881 aagtattatt ttcaaaagca attattagag catcatttac ttttgatggt gatgagatat
    17941 tgtaaattgt ttctatatga tgtctaagcc cagaatcatc actatcattc caatcattta
    18001 atttatcatc ttttctccaa ggtaacttac ctaaaacgac agctctattt gaaaattcat
    18061 tataagctat ttttcctttc aaatatgggt cattttctat aaacattaag atattgtttg
    18121 ttgttttctt ataacttccc ttattgtcat aatctaacct agttaaccat tcatcatctt
    18181 caaaatctat atcaccaaaa tcatccttag ctttatcaag gttttctctt cctatagtct
    18241 ttcgtacttt agtgtcactg cttgcaaatt cgctcattcg agtaaatgaa ggtaatctat
    18301 ttacaggcgt ttcgggttta gcatcttcat caagttcacc aaatttatgt atcctaacta
    18361 aatcaaaagc attgcataaa attccgcttg ctgggtctgt accatgatga ctatatgaaa
    18421 acttgtcatc ataaataact actccaccac ttgtactacc ttctgaatat gtatacctgg
    18481 tttcatcaat accaggaata tatacttcat ttaagaaagt ttctatagtt tcttttatac
    18541 tataggacct gcaaaatgca cctataattc cagccttttc aatagggtct tgttgttttt
    18601 ttaattgtgt attaaacttt tgcctctccc tcgaactttc tggccaatag cttacatctg
    18661 tccagtctaa atataaatct agtatttcat ttgggtttaa aaactctcca tcttgaaatt
    18721 taaatatata gtctccatca attgaagtac ttggaaaata cataagtcta tgaggttgat
    18781 atgtagtatc atcaaacata tctattccta tagtatctgc tatcatcctg gaaattgctt
    18841 gatattcttc tggtagtact ggtctagtta aaggtattac aagtctatat ctgggattat
    18901 tctcagtatg tgagtgcgta gaatacataa gacaagcata atcatttaat aatgttatat
    18961 cctcccatat atctttattc gcataatcta tatctaaagt tataatactt ctatttgcaa
    19021 tattctctgc ttttcgtctt ccatttttta agcttccacc tacaaatcca ccaacatctt
    19081 taactctatc tttttcagtc tttgacatct tcctatattc tgtatatgtt tcttgagttc
    19141 ttaatgtttt acttaatcta tttacaagtt cggaccataa aatactttta tttttccaat
    19201 gtgtttctaa tttatttttt cctatggcca gcatgagttg gccatcatgt cttacattta
    19261 tgtgttcaat ttcactggcc tttatatcca taaatcaatc acctagtctt tcttataata
    19321 atcacattca tatccatctg ctttaagagg aagaccttta gcccaagata tttcttttcc
    19381 cataatactg ttaacttctt ctaaagaacc tcctttttta tctacatcaa ttacaagctc
    19441 atcatgtaca tgcattacaa tgctataacc tgcatctgtc acattaaaca tagcctctct
    19501 taagcaatct ctagctgtag cttgaacaat attctcaact aacttaggtc cataagtatc
    19561 tattcttttc cattgtttac ttgtctgttc cataccttca tatgttatct tatccccact
    19621 aaatgtagta tgtggctcta tcttaggcct tagatacgat aatcttctac tacttggtag
    19681 ttctataaat aaaacgcctg gattataaat aaatttaatc ccatgttgaa tttctactat
    19741 agtcctatct tttatagctt ttttagctgc cttatctaca tcccaccaaa acttagttat
    19801 atttggatta gcatttctcc atgcagtaac aataggttga agctcttctt ctttaagccc
    19861 cattttaata gcccccatag aaattaaggc ccctacactt ccgccatatc cattcgataa
    19921 ttctgctagc tttccttttt gtctaagttc agaacctttt ttaatatttt cgattggaat
    19981 tttaaacatc tgactggcac tagcttcata tatttttcca tgagaattaa acacatccag
    20041 tctccacttc tcacctgcaa gccaagctat aactctagct tctattgcac taaaatctga
    20101 tactataaat ctatgacctt cacttggtat aaaagctgtc cttatcaatt gacttaagac
    20161 atctggtaca ctatcatata aaagctctat taaatcaaaa tctccttctt ttaataggtt
    20221 tctagctagg tctaaatcct ctatatgatt ttgtggtaaa ttctgtactt gtactaatct
    20281 tcctgcccat ctaccagtcc tattagcccc ataaaactgt agtagacctc ttactctatt
    20341 gtcattacct ttagctaatt tcatagcctc atatttcttt atagaagttt tggacattaa
    20401 ttttctaagt tctaaaattc taactacatt ttcatcatca acttgtttta atatttctgg
    20461 aatactttct tttgttaggc ttgtaatctc aaagccaact ttatcactta accatttttt
    20521 taattgagct ggactatttg gattatttag accagttatt ttaattgctt cttttgtaag
    20581 tttttcagta tatcttttat cacattctat tgcattctct attaactctg tatccacttt
    20641 aataccagta tcattaattc tctggtctaa ataccataat ttaatttctc tttcggtagt
    20701 cttatactta ctaagtttgt ttcttatttc tctttcaact acaacatctt gtttacaata
    20761 ttctttaaat ttattccatt tttccatatc atgtattggt aaatttctag ttcttccctt
    20821 gttaactttt gtagccttac aaggtttaca gaaatattgt attaatgctt taccttcttt
    20881 cattttctgt ttatcttcat taaactttaa agccttagac acactatcta aacttcctgg
    20941 aagtcctagt gttaacgcct ttatcattgt acatgaccac tcatttggtt ttaaattaat
    21001 atttaaaaat ttacttattg atgttctttc aaaattagca ttaaatgctg attttataac
    21061 tttattatca tttaaagctt ctattacttc ttttggtaac tcttcatcat ttactaaatc
    21121 aataactttt acctcttcat tatcaaaagc ataggcaaat aacagtatct caaaattagc
    21181 agagtctaca tatctgtaga ctccaacttt ttttatatct aaatcactat atgtttctat
    21241 atcaattgat aaggtcctca ttaacttaag aagtcctctt cttcatcttc atattcaaag
    21301 tcatctgaga aatctgcttc tgcactagct ctagctcctc caagtacttc tccatctgct
    21361 aacttttgaa cattttgtaa gccacaacct attcctttat ttcctgcact attatatggg
    21421 aaaaagttta tactaactct tccataacaa ccactatata cctctgtatt atctaatatt
    21481 tcatttaagt ctttatcaac tattcctggc ttttgagtac tatttgcatt taaaaaatac
    21541 attcctacat attcctctgc ttcatcagct ctttctgcat ctccatcacg aagaggtgtt
    21601 tttaaattac ctggtaattt tccaccccat ttagaagttt taccttgttc tttagctgca
    21661 tcaatagcct tctttattct tcctaaagtc accttatctg attttggtat taaaatacaa
    21721 actgaatact tgggctctgc accttctacc attgctctgc ttttaaagat attgcaataa
    21781 cttaatctta cctttcctgt tactaccttt gttgattgta ctgaattact cataattttc
    21841 ttcctcctaa tatttttata tttggtatat tgattttata tttgaagtta tttctccaaa
    21901 tttatctaaa tctctaactt gcttactaaa caatttaatt cttccacaaa taactgttga
    21961 agaaacattt aataactctc ctatatcaga tatcttttta tcttgtaaaa tccaatctac
    22021 tattttatca atatctttta aatatgactt tcttgcaatt tttcttatat aatcaacatc
    22081 atagttaaat tgaatttcat aaaatccatg tatatctttt aatggtcttt tactatcttc
    22141 aaatacactt acatctaaac cttctataga gtttctataa tgtttttcgc cataacgcaa
    22201 agctttaaac atttctattc ttatgtaaga aacagccact gtagaaaact tgcctctgtt
    22261 actatcataa tttaatgctg ctttatataa cccaatacac ccttcttgat aaaactcttc
    22321 atataagtat ggatgttgct ctacataaga cttaaaaaat ctatttatac taaaatgtac
    22381 aagattcaaa ttattttcaa ctagtttagt tatttcttga ttactcataa aattatatcc
    22441 ccttttaaat ctttttatag ctactaggaa ttaaatattt ttaattatcc tttgctctta
    22501 ctgatacaaa gcatactgga ctaactttta tatgttctgg tatatacact ctatcaagat
    22561 gattgtcatt tacttcgagg aaagttgaca tcactttatc ctttattaaa ctttctcttt
    22621 taaaatccat cactataact tttgcatttt cttcagttga gttatattct gtaatattta
    22681 gtatctttaa catatctagt aaattatctt tttcataatc taaatctttt tcgatactct
    22741 tttttctatc ctttaaatct aaaattatac ttatgctttc atctaattta tcttttatac
    22801 ctgcatctaa tggcatgtaa tatcccccat ttatatttca aaatcttttt tcgctgaatc
    22861 tatactatta atttctgacc ttttatcact ttctactact aaagtagctt ttccaactgg
    22921 ttttataatt aaatcactta gtagtttagc aaaactcttt ttacctatag ctttctccat
    22981 atcactaatt ccttttaagg tccttggctt ataaattttt tcttcatcat aatctgaatt
    23041 taataaaacc ttagcaactt cttgctcatc tacatacttt ctattacttc tgccttctac
    23101 aagcttatat cctggatatt taacaccatg cttttcagct tgttctagtg cataactttg
    23161 aacatctttt aaccaatctt gtatattctt agcaaaacct aaaatatctg ctatctcata
    23221 tttattaaga gcaaatgtgt cagcaaaatc atattttcta gctagtttaa ggttatcctc
    23281 agctcttttt ctgcaatcat ttttagctct acaaaatcca caatgactgc cacttacaaa
    23341 ctctccttca ccattaaaag ccatttgagc cttcttctta acattatctg cccatgtaag
    23401 taatttagta acttctattt cttcacttga tatgttatca agtctaggtt gaattattgt
    23461 tgttttaatt aaatcaatat cgtatagcat ttcaaactga ttatatgccc ctaaaccata
    23521 tagtctaagt tgaggattct ctatagctga aacttctaaa ccttttccat actttaggtc
    23581 tataacttga agtataccat cagatattac aacaacgtct cctgttccaa atccttctgg
    23641 aacccactca ctaaaatcga gtctttcttc taacatcact attacatcat cacatatagc
    23701 tttactatca tttactaatt caactacatt ctcaacataa gattgtatat agtcctccat
    23761 ttcagagtta taatactcac ttttctgtat ctttttaatt cttgcattat atgccttttt
    23821 acttattttt tcatactcta gcattaattt aacttctgct aattcatgtg caactgttcc
    23881 ttcttctgca tatatactag ttgatggtgg atagttttct tctaatttta tactaggagt
    23941 gcagtgaagc catctatggg ctccactcgc actaagtctt gcatgttgta atggcattta
    24001 tgtaccccct ataaactttc taatttgttc ataaaagctg aataatcttc ttcttttact
    24061 tcacttaact tactagctcc aaattcacca aataactcct taagcttatc cttcttacct
    24121 gccttactta cttgtgctgc cttagttctt acctcttctt ttgtgtattt aacttcacta
    24181 gttgtatttt cattattctt ttctatttct tcctttgctt ctgctatttc ttctttaacg
    24241 tcttctttta cttctacctt tttaatcttt tcagcttttt taacttcaac tttcttctct
    24301 tctttcatat ctgttatatt tataggttct atctgcattg cttttcctag attaagacct
    24361 cctaaagcat tcgccactac taatagtgca tttgtaaatt ctggtgcttc tactttaact
    24421 tttacattta cattcacttc aaccatttta aatctctcct ttttgtgtta tactttactt
    24481 gtgttatatt ttatttttta tttttttgtg tgttggttat tttaccaaca cttttttatt
    24541 taatattcca accgatattt tcttaccagt tttaatatct ttaaatacta catctgctat
    24601 aacttttcca tcttttttaa gagttactac attcttatta ttagtatcaa gactcagcaa
    24661 tttcatcccc cctctctact gcttttaaaa gttcatccaa atttttacct tgatttcttt
    24721 caataaaatc atcaacttca tatcttgaaa tttttctacc atcacctcta actagtgatt
    24781 ttatcaaacc tgtgcttact aacctacgca taaaagctgt atctaacttt aaaattcccc
    24841 ttgcttcttc tactgttatt agataatttg gataacctct ttttattaaa acaactatat
    24901 ctttaggctc cagtaccttt accttttgtt caatagtttg actcttaact ctatctgtct
    24961 cttgtttact tatctctatt tctattaagc tttttaagct atcactaaat tgttttgtta
    25021 tgctttcaga aatatccatg tgaaaatgct ctcccctctt ttcaattatt tatcttccaa
    25081 ccaattcgtc taatgtaata tctaaataat cagctaattt tattagagta tctatagttg
    25141 gattagaact tttatttctc agtaatttat ataaaccact tacatctata tccaattctt
    25201 ttgccaattt ataaggtttt atatctcttt ttcttagtat ttgctttata ttgtcgccta
    25261 ttaacattgt attcacctca gttctacatg gtataatata aacattgaat aaattccaaa
    25321 attttcaaaa ggatggtgta ttatgcagtt ttcaaacaat atatctaaag aattacaaga
    25381 aagtataaga aagacagtct ctgatacttc taacacagag tatttttact atgctgaatt
    25441 tcaatataaa attattctta aatctataga ggaatttgaa aaagaacttg atgatgaaca
    25501 tgaaattgca ctaaaattga ctaactttgg aaaagatgta ttaatgattg ttgaagaagt
    25561 aggttatcat aacccttgtc taattcacta ctacggcata gtaaatggag tttattctga
    25621 aattcttcaa cacacatcac agataaactt tatgataact tcagtaaaga aaactgaccc
    25681 ttcaaaacct gctagaagaa taggttttat attgtaatta tcattcttat tttctgattc
    25741 tttctttagt aatttcacta atttttcttt tttcactttt attcatctca tgtaaataca
    25801 aaactataca ttggtacata taatctataa aatcactatt tgattgagct gttatctgac
    25861 taaataactg tgcttgcaat tgattttcaa gcatggttat tcttttctct atttcttcta
    25921 ttttcttatt ttccaattca atctctcctt tccaaaatat tctgtattta gttttcaaag
    25981 tactaaccta atattgaagt ttgataacta tattcttttt ctatatctgg taatatgtta
    26041 tttgttttca ataaatcata taaaaacaat ctaccttttt gagtccactt agttgtcatt
    26101 tttacatcag gcattccatt acttctagtt atgtctattg tttctgaatg agtgtatccc
    26161 ttcccttgat gttgtttata taaaagccat tgtccacttt gtttatattg aatccctctt
    26221 tcatgaagta ttttgttcat ttcttttcca ctcattccat aatcctttgc tatttgagtt
    26281 atagtgacta gacctttgtt tttaagtatc atatctgtat aatctgcctt tggttttagt
    26341 tcttttatta ctttttcttt cattttactt tctagttgta actgctcttt ctccttttgt
    26401 tcctctatcc aacgtttagc acgctctata ggattatcta tcatataact atcttttaat
    26461 tgtcgattta ccatgttata tgtgccagtt ttacgaatac ttggtagaac ttcatctgta
    26521 acccaatctt gaaatctctc tgcttcttct tttcttgatt taaagattaa cttatacaca
    26581 ccactttcag taagaaaatt ttctcctgta ttgtgtagtt ttctaaagtc agtactactg
    26641 attttagaat taattaactt aattacttgt ttattattca tatttctcaa attttcattt
    26701 acatttttta tatctaagca ctctgcaaca tgttttgggt taaataaaat ctgcccttca
    26761 aactcaaaca cttctacttc ttttccttca aataccatta agttattact cataaattct
    26821 ttccccctta attatttata gttgattttt ctaaaagctc atttgatact ttcataccat
    26881 ctgccattgc agctatttat tctgttttta gttttcaaag tactatcaat ctttagccta
    26941 ataaagttat ttgacactct cgttcttttt ctctaattaa tccatctttt ataagtaaca
    27001 atctaaatgc ttctctacct tttggattta ttaatgtttg agtatctgaa tgcccgtatg
    27061 gtgttgtaaa ttctttcagt tcaaagtact gcatcttatt agaataaggt tttatctttc
    27121 cttttaaatc tctgtaacag tatttcttct ctattaacca taaaacaaat actttttctt
    27181 ttactccaag ttcttttgat gtatctctta tattagttag taaatttctt tctactaaag
    27241 catcaaagta atctgcctta ggtttcattg tttgattttc tagttgtagt tgttcttttt
    27301 cctcaacttc tattaataac tgttgtaatg cttctttata tgtagttggt aatttagttt
    27361 gttcattttt taaagctttt tccatttcat caaatttttt tgtatatatt cctgtaaatg
    27421 ctgtaccttt tatgcctgtc attttatttg ctataaagtc acaacctatc tttgttattg
    27481 cgtaacatgg tttctttctt ttataatcat ctaaataagt attttcaata aaataatcag
    27541 atgggtacaa atctgtactc atgtttttat ttgcttcttc catttggttt atataatttc
    27601 ttatatccct tagtaaaatc ttgtgttctt tttcaaccat tagagctata tccctactat
    27661 cagttgttag tttattatta acttttacta tttttaaatc tttcatattt acaaactcct
    27721 ctctataaaa tgtccatcat tttattgcca ttttagcaac taattctgaa aaaaaatagt
    27781 ctgttgatac tccatataat tcagacaact tttttatttc atttgctttg aactcatttt
    27841 taccattaag tttaagccta aaaccaaata aactaagacc taacaaatct gcaacaaatt
    27901 tttgcgtatg ttggttttct ttcattaacc ctttcaatct attgacatac attttttcac
    27961 cctctaactt tttgctatat aagcaagttc ttgtttatat aatagcaaca aaaatgttaa
    28021 aagtcaatat ttttgttgct tttttggcaa ttttaattgt aataacaaaa aaagttgcta
    28081 taatgtaaat aaaagttgct ataatgaaaa ctacaataaa ggatgatttt atatgtttag
    28141 attaaaagaa ctgagagaag aaaaaggaat atctttagat agattaagta tggatttaaa
    28201 tgtaaataaa tccactctat ctaggattga aaatgggtta agagagccaa aacaatcttt
    28261 tattgaagac tgttcaaatt attttgatgt atcaacggat tacttacttg gtaaaacaga
    28321 cataaaaaat cccaatcaag tagtaaaatc tttaaatcct attttctcaa aaagattaag
    28381 agaattaata aaggaaaaag gtgtcacatt aaatatatta ggcaaaaaat tagaaataag
    28441 tactgattta ttatctaaat atgaaaataa tataatttca cctaaaagtg atgatattat
    28501 aaagcttgct cattattttg atgttacaac agattatctt ttaggtacaa cacttgttag
    28561 aaatcatata gacactgtag ccgcacacaa agcaaatcca catgaggatt tacctgaaga
    28621 agctcaagaa cagcttaatg attatattga atttttaatg aataagtata aaaaataaat
    28681 taaaagtagg tgataaatta tatgaaatct ataatatcag atgtcataaa ccgatttaat
    28741 gctacaccat ttttatttgt tggttctggt ttaactagaa gatactataa tcttccaaat
    28801 tgggaggatt tactaaaagt ctttgcagaa aaaattagta atgatgactt tatatataca
    28861 agttataaaa ataaagcaaa atcagagaac ccaaaaatgg gtataaatcc tagaattgca
    28921 gaacttattg aaaatgattt taataaaaaa tggttttcag attcttctat acgaagctta
    28981 gactctaagt atttagatat tgttaaatca ggagtttctc catttaaggc agaaattgct
    29041 atgtatatta aaaataattc taaaatagta gataaatatc aagatgaagt tgaaaaactt
    29101 agtaatatct ctaaaaaaag tttatcagga tttattacaa caaattatga ttgttttttg
    29161 gaaactatcg ttgataatta tacaacatat ataggtcaag aaaatttagt tttttcatct
    29221 atacaaggta ttgcagaaat ttataaaatt catggatgtg tttcttctcc aaatagtatt
    29281 gtaataaatg aagctgatta tatagacttt gacagcaaaa gtgcctactt ggcagctaag
    29341 ctaatgacta tatttgttga gtttcctatc atatttattg gttactctgt tacagatgta
    29401 aatataaaaa aaatacttaa tgctatagtt aattgtctat ctaatgaaaa tgctaaaaaa
    29461 ctcgaggaac gtttcatatt cattgagtat gaaaaaaact ttaatgatat agaaatatca
    29521 agtcatacaa ttgcttttga taataaaatg attactatga ctaaaattaa actggaggat
    29581 tttaatttac tctatgaggc attatctgaa aaaaaatcaa aattaccagt tagaatatta
    29641 agaatgttca aacaagaatt ttacgatttc acaattacta acaaacctac tgctaaaatt
    29701 cgggttggta gtgttgatga tacaagaata aaagatgaag atttagtttt agcagttggc
    29761 aaggcaagtg attttggtct taaaggatta aaaggattaa gttttgatga atggtatcgt
    29821 gatatagtca tgaatgactt agaattttca tcagatgaat tacttgaata tgcttatcca
    29881 tctttaatta agcaatataa taaattacca ttaaataaac atctttttaa tagcacactt
    29941 gaatttccag attataggac aattgcacta gaaagtgact ttgaaaatat tattagcaat
    30001 agtattaaaa aaaatagaaa taatacttac ataaaaaata gaagtgttta tggtatttgg
    30061 aatgatgaac atcaatcatt tgaaaaagct acaagactta ttgctttttt agaagaaaaa
    30121 gaaattgaca ctgaacagtt agaaaatcta cttaagaaaa tatttgagga aaatcctaat
    30181 attttagagt ctgcaaaaac aagcgaaaaa acaaatttaa gaaggcttat tagaatatat
    30241 gactacatga aatactctaa tatgcaaaag agtcttggtt atagtgagta aaactctacc
    30301 caccgctcca aaactccttt aatcgcttta agcacaaata caaatttagt gctaactata
    30361 ctaacacaca tttgtaagtg cttacatata ttatatgtaa ttatattata atcatatact
    30421 ataatccata ttttgtctac agctaaaatt atccaatctt ttttggataa aaaatgaata
    30481 tttagagcag ttcacacctg ctctttatat ataacaaaaa tcaaaaatac attcttttta
    30541 tagggggatt tcaatgaaca aactagacgc acttttagac ttagcaaata atgaagagat
    30601 agaaatttac tacactgaca aaatagcaga tgacataaaa ggattgtata taaacagaca
    30661 aggactaaag attatatcat tacttaattc attaaaacaa aacaatgcta aactaataga
    30721 aatcttagca gaagaattag gacatcattt taccagtgtt gggaactatg tatcttcaaa
    30781 aaacagttac aaaaataaaa tcttgataga caaaactgaa aacaaagcat taaaatgggc
    30841 atgtgaattt cttataacag aagaagaaat aatacatgtt attaattcac acgctacaag
    30901 tgtatacgaa atagctgaag aattacaagt tagcatcaac ttcttactaa aaagattaga
    30961 atttctatca aaaaagaaaa gcatgttgga cttaggaaat aatagatttt tagtattaac
    31021 taatttgcca aatttctaca tatatgagga tattt
//
LOCUS       DAEPXF010000027        30442 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain HB27
            SAMN13376389-rid12479423.denovo.028, whole genome shotgun sequence.
ACCESSION   DAEPXF010000027 DAEPXF010000000
VERSION     DAEPXF010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13376389
            Sequence Read Archive: SRR10518432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 30442)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30442)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001069624.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001069624.1
            Genome Coverage       :: 315x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/15/2021 01:54:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,165
            CDSs (total)                      :: 4,076
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 89
            rRNAs                             :: 10, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 10 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 19 of 69
            Pseudo Genes (incomplete)         :: 44 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 10 of 69
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30442
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13376389-rid12479423.denovo.028"
                     /strain="HB27"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="China: Hebei"
                     /collection_date="2017"
     gene            32..1144
                     /locus_tag="KRL67_001127"
     CDS             32..1144
                     /locus_tag="KRL67_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5009 domain-containing protein"
                     /protein_id="HBH1522026.1"
                     /translation="MNSRIKSIDIIRGLSIALMIVCNNPGTWMRMYPQLRHAVWHGVT
                     LADFAFPFFVISLGVTIPISINSKIKNNKSTLSIILSIFKRSILLILFGFFLNYLGNP
                     DLDTVRILGVLQRMGLVYFVTSLVYLLLKKLNVGSTATIITFLCISTFIIVGYYILAK
                     PYGFELEGSLAQLVDLHFFKGHLYKPEFEPDGFLTSIVAISSGMLGCTMGCVLLKEDI
                     GEYKKFFKILVMSIILLIGAFIFNQYFPFNKRLWSSSFVLLMAGSYGILLSIFYFICD
                     IKNKSKIFTPIIALGSSPIFTYMCLEILSHVFWNVPKLTNKVDYPTTLVEWTTYELIT
                     PWAGTTWDSLVFSLLYVLFWVIVMSIMYKKKIFIKI"
     gene            1398..3422
                     /locus_tag="KRL67_001128"
     CDS             1398..3422
                     /locus_tag="KRL67_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HBH1522027.1"
                     /translation="MKKTTKLLATGMLSVAMVAPNVALAAENTTANTESNSDININLQ
                     RKSVVLGSKSNASVKFKEKLNADSITLNFMCYDMPLEATLNYNEKTDSYEGVINYNKD
                     PEYLNVWELQSIKINGKDEQKVLNKEDLESMGLNLKDYDVTQEFIISDANSTKAVNEY
                     MRKTSAPVKKLAGATRFETAVEISKQGWKDGSSKVVIVNGELAADGITATPLASTYDA
                     PILLANKDDIPESTKAELKRLNPSDVIIIGDDGSVSQKAVSQIKSAVNVNVTRIGGVD
                     RHETSLLIAKEIDKYHDVNKIYIANGYAGEYDALNISSKAGEDQQPIILANKDSVPQN
                     TYNWLSSQGLEEAYYIGGSQSLSSKIIDQISKIAKNGTSKNRVSGADRHETNANVIKT
                     FYPDKELSAMLVAKSDIIVDSITAGPLAAKLKAPILITPKTYVSAYHNTNLSEKTAGT
                     VYQIGDGMKDSVINSIASSLSKHNAPIGPDNSGSAAGKTVVIDPGHGGSDSGATSGLN
                     GGAQEKKYTLNTALATTEYLRSKGINVVMTRDTDKTMALGERTALSNTIKPDLFTSIH
                     YNASNGAGNGVEIYYKVKDKNGGTTKTAASNILKRILEKFNMKNRGIKTRTLDNGKDY
                     LYVLRTNNYPAVLVECAFIDNKSDMDKLNTAEKVKTMGTQIGIGIEDTVK"
     gene            3642..4328
                     /locus_tag="KRL67_001129"
     CDS             3642..4328
                     /locus_tag="KRL67_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HBH1522028.1"
                     /translation="MSGYTNDECEIPKIKSYPGADKEIASEIDYSIVKGTVLFDLYQR
                     IMDLVLSIIGLVIGLPLIAIFGILIKIEDKGPITYKQERLGKCGRRFYIYKLRSMRTD
                     AEKFGAQWAEKDDPRITKVGKFIRKTRIDEIPQLFNILKGDMGLIGPRPERPNFTVQF
                     NEEIPGFINRLAIKPGLTGWAQVNGGYEITPEEKLKEDIYYIKNRSILLDFKILFKTV
                     KVVLTGDGAR"
     gene            4423..5478
                     /locus_tag="KRL67_001130"
     CDS             4423..5478
                     /locus_tag="KRL67_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp7"
                     /protein_id="HBH1522029.1"
                     /translation="MIKKISTILSLVLLISISSTIGVFADANPKRELIEGSIPEISTE
                     LNKRAFKDSKEVILVNEESIVDSISATPLAYAKNAPIVVTKSKNLGRVTRNYLKELGP
                     EKVTIVGGLKAVSKDAERNIEKMGIKVERIRGKDRYDTSLKIAREMYRTVGFDEAFLL
                     SSTTGLENAISVYSYAAKSGMPIIWAKDEDFEEQIDFLKGKNLKKIYALGDSKEFIAE
                     IDSNLKNIEGIKQINKSSTNVDLIKKFYDEKDIKKIYTAKLDFGSRSDVNEYISLGVV
                     SAKENMPILICSDNLSRAQDKFLKDSNINDVVEVGYTVGDYSLFKSIFNLTFLSCIVL
                     ILLLLLITFRALRYESK"
     gene            5593..7152
                     /gene="murJ"
                     /locus_tag="KRL67_001131"
     CDS             5593..7152
                     /gene="murJ"
                     /locus_tag="KRL67_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HBH1522030.1"
                     /translation="MSKTAKAALWIMAATMFSKVLGFLRELVLANFYGTGMYADVFVL
                     TLNIPGLIIAVIGSAVATTYIPMYFETKKRLGDEGALKFTNNVLNICYIMAIVIAIIG
                     LLFTEQFVTIFAAGFRNDPAKFQAAILFTKIMISGVLFLSGSKIFSSFLQVNDSFVIP
                     GLIGIPYNIIIIAAIALSAGKNVWILPAGALLAMASQLLFQLPFAFKKSYKYKPYINL
                     KDESIKELVNLVLPMLVGVAVGQLNIFVDRLLATTLGDGKLSALNYANRLNEFVMALF
                     VTSIITVIYPKLAKMSGKDNKEGFISTIVKSSNCIILVVLPISIGAIILAEPLVRILF
                     QRGKFDALSTDLTSIALRLYSLGLLACGVRDVLYRAFYSLSDTKTPMINGSIALIINI
                     VLNLILIRPLGHAGIAISTSTSNIITVILLFISLKKKNGYFGGDKIIKTGLKSLVASG
                     VMAVATLLIYNNLYAFMGSGTIKEIISVGAGVLGGASVYTVLIVLFKVEEMDLAFEFL
                     RKGKQKLLRRR"
     gene            7194..8900
                     /locus_tag="KRL67_001132"
     CDS             7194..8900
                     /locus_tag="KRL67_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HBH1522031.1"
                     /translation="MDYKNNYEMWLNSPYFDEQTKNELLSIKDDEKEIQDRFYKNLEF
                     GTGGLRGIIGAGTNRINIYTVRRATLGVLNYIMKTQGEEGKQKGIVIAHDSRYMSREF
                     CIEVAKTLSAYGVKAYIFEELKPTPELSFAVRYLKCAMGIVITASHNPKEYNGYKVYD
                     SDGGQICIDMANDIIAEVNKIDDYSTIKSIDFEEALSKNLITILDNEVDDEFIKAVKK
                     QVLRQNIIDEYGKKLKIIYTPIHGTGNKSVRRVLSECGFKNVMVVKEQELPDSNFSTV
                     KYPNPEEKSVFNIAIEMAKNNGTDLIIGTDPDCDRVGIVVKDSSGEYVVLNGNQVGSL
                     LVRYILESLVEENKLPKNNPTIIKTIVTSELGAKIAKAYNVDCLNTLTGFKFIGEKIK
                     AFEESNDRSFIMGYEESYGYLIGTHARDKDGVVSSLMICEMAAYYSSKGMNLYEALID
                     TYNKFGYYKEDLKSVTLKGIDGIKKIKEMMLYFRSVKIDNVADVKVDKILDYKDGVDD
                     LPKSDVLKFLLEDGSWIAIRPSGTEPKIKFYFGANSDNQEDVEFKLNNLISYILNVVD
                     SI"
     gene            8922..9995
                     /locus_tag="KRL67_001133"
     CDS             8922..9995
                     /locus_tag="KRL67_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate guanylyltransferase"
                     /protein_id="HBH1522032.1"
                     /translation="MKVYNVIMAGGGGTRFWPLSRQEVPKQLINLSGEDALINETINR
                     IDSLAKKDDLFIVTNEKQLEALKDIVKDKCLDSNILPEPCARNTAAAIGFAAFNIMKK
                     YGDGVMCVYPADHYIKDEKEFKSILEKAIYIAENNDKLVTIGITPTFPSTGYGYINFN
                     RENTIEDVAYEVVEFVEKPNYEIAKEYVNSKKYVWNSGMFVWKVSKILEDFKRYLPKV
                     YEKLEDISKYLGTKEEMEKIKEIYPTIQSISIDYGIMERSNDVIVVPGDFGWNDVGSW
                     DSLGAIYPTDDEGNIKRGENITIDTKNSIIYSDDKLISTIGISDLIVVSTNDAVMVCR
                     KDKAQDVKKIVEQLKEEDRQEYM"
     gene            10007..10729
                     /locus_tag="KRL67_001134"
     CDS             10007..10729
                     /locus_tag="KRL67_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HBH1522033.1"
                     /translation="MIPKKIHYVWFGGPKGNIENICINSWKEKLPEYEIVEWNEKNFD
                     IEKEIKGNKFLEECYKRKLWAFISDYTRIKVLYEQGGVYMDTDMQILKDITPLLENNR
                     LICGYEDDREYINGAIIGVEKGHPFLKDLLEYYEKEVLTSSLFTIPKIMTHLMEKNYK
                     KIDPNNYEEGIRVYDKEYFYPFGFKEDFTPECITENTFGIHWWGKSWAKKRNYFLESK
                     HLTGVNKIWKCCKIFASNTLRS"
     gene            10745..11902
                     /locus_tag="KRL67_001135"
     CDS             10745..11902
                     /locus_tag="KRL67_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide polymerase"
                     /protein_id="HBH1522034.1"
                     /translation="MKTLNDDRNIMIRKYLYIFVLFIILLRKYMISFIDPNMDIGMIK
                     SALFYSSIGILMLLFLFDKRKSITEMVLVGVCVLLYLLNREGAILLIVLLAVSAKQID
                     DKFIVKNYLIISACFLMVAILLFNLFPSLIFNQEVHYRYIEKIDMLVTRMDFGLGNPN
                     SVFYHMVTIYAAYIFLRYKDYNKWDRIILFGSAFFVYQTTYSRTGFFTILAGLIFVEI
                     IRWIDIKKIKGLPMLLKTLPIILTLFSVIIGTVFDKSTLLNRLLASRPKFWHVYLAEK
                     GNFLKPFGNSYSPAIKATNPLDSSYVYIISVLGVVACILFMYLMYKGIESFIEKDKKA
                     YLVALFIFLLYSFAENLLLEASFSFAVVLLIKEVILNDKREIDLWKMKSRR"
     gene            11905..12744
                     /locus_tag="KRL67_001136"
     CDS             11905..12744
                     /locus_tag="KRL67_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HBH1522035.1"
                     /translation="MLISLIMPTLNRYDDIYLLMDSLENQTYKNFELIVVDQNDNSKV
                     KEIVDKYIDKLDIKYIKSSKKGLSYNRNVGIDNAVGQIIGFPDDDCVYENDTLEKVIN
                     FFNKNKDYKIYSCKTMDSNKVDAFKKMYDGTCDITSSNVLDTITSITFFIDFEGKDYT
                     RFDEKLGVGGEFGAGEEIDYVLNLLSLGFKGKYFGNDIIYHPAKKHSKSKEKYQKDYN
                     YGRGFGALCKKEIVYRKNYKFAKVMVSKLVRNIGGLILSSNRDYHSATIKGRINGFRQ
                     YKL"
     gene            12759..13934
                     /locus_tag="KRL67_001137"
     CDS             12759..13934
                     /locus_tag="KRL67_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-glycerol glycerophosphotransferase family
                     protein"
                     /protein_id="HBH1522036.1"
                     /translation="MDKVKFLINMLLAFFIYPFNKHKFNGRNIWLIGGHSGDIYNDNS
                     KFFYEYMLKEHNDVETYWVVNKDSKVFDKIPGKKLIRGSVENYLYYYNSKAIVFSHAP
                     SADIAPYNFAVPVLNYFHKKTIKVFLNHGTISFKKRKPMNKKFKNIIDNLYKSYNIVT
                     ASSEFERNVMVNDWGMLDDSVYIIGNARYDNLPTNEVAQTRDILYTPTWRDWIKFSSG
                     KFTDTDYFKNIMNFLNDDKLNKILDEKDINVKIYMHHLMHEFIDDIKENITGKRIVFL
                     DKGVTLANEIRKSAANITDYSSVAIDFLYMNRPILFYQFDLDEYMEKVDSYIDLKSEM
                     FGSLAYNNDEAVNKLIDIIENNFEVMDNQKNERNKFFRYNDNKNCKRIYDCVLSKIK"
     gene            13963..14712
                     /locus_tag="KRL67_001138"
     CDS             13963..14712
                     /locus_tag="KRL67_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HBH1522037.1"
                     /translation="MKKNLVSIITPMYNSEKFIEATIKSVLNQTYQEWEMLIIDDCST
                     DNSPNIVKSYMQQDSRIKCIKTETNKGVSNARNLALSKATGQFIAFLDSDDQWNSSKL
                     EKQVNFMLENDYVISFTSYELMDENDKKLNKVIKVPPNVDYKRLLKGNILGCLTVVID
                     KSKLDFEIRMSGVRHEDYVLWLSILKKGHIAHGINEVLALYRKSSNSLSGNKIKAAMW
                     TWNIYRNIEKIPLYKAIYYFINYGINGIKKS"
     gene            14734..15642
                     /locus_tag="KRL67_001139"
     CDS             14734..15642
                     /locus_tag="KRL67_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HBH1522038.1"
                     /translation="MYKYKFTVFTPTYNRAHLLENLYNDLKAQTYDFNDFEWLIVDDG
                     SSDSTKELVEKFISENKLNIRYIYKENGGKHTAINLGVKNADGELFFIVDSDDGLIKD
                     SLKIANDEWDSLENKEGFSGVVGLCVYPSGDLIGTEMPEDKKICHFADLYFKYGVKGD
                     KSIVFVTDELIKFPFPERKEVRFLPESVVWNEMSKYYKVICVNKPMIIRDYLDDGLTK
                     NILSKNALRGRALEFLYLINQNTYPLSRYPYMWIKNYINLARYSLLSDSHYFGELRKV
                     SDKLLYLALFPLGYYKYIGQRKLVSK"
     gene            15690..16442
                     /locus_tag="KRL67_001140"
     CDS             15690..16442
                     /locus_tag="KRL67_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HBH1522039.1"
                     /translation="MNEPLVSIITPVYNSEEFLSETIKSIQNQTYKNWQLLLVDDCSK
                     DNSSEIIKSFRKEDARIKYIKLEKNSGAAVSRNVGIKNAEGRFIAFVDSDDLWDSRKL
                     EIQIEYMLKENVGFSFTSYRYMRQDGSKTNKVARAPKKIDYEGLLRNTIIGCSTVVID
                     KEIVGEFSMPLVRRGQDTATWLQLLKKEKYAYGIQEDLVNYRLVGNSISSNKIKALKR
                     TWNTYRNVENLSLPKSLYVFCFYVFNAIKKRV"
     gene            16420..17763
                     /locus_tag="KRL67_001141"
     CDS             16420..17763
                     /locus_tag="KRL67_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose/GDP-mannose dehydrogenase family
                     protein"
                     /protein_id="HBH1522040.1"
                     /translation="MPLKKEYKFYILGGKKMKIAIAGTGYVGLVTGVCLAEVGNENVV
                     CVDVDEQKVKSMKLGIAPIYEEGLEELMVKNYNRGRIDYTTDYKKAYEDVDVILIAVG
                     TPERSDGSANLDYIKAVAKQIAETVTKDTLVVIKSTVPIGTNEEIEDYINRNLKNDVK
                     VELASNPEFLSQGTAVKDTLHASRIVIGVESEWSREILEEIYKPFNQPIIVTNRNSAE
                     MIKYASNNFLALKISFMNDIANLCEIAGANIEDVALGMSYDDRIGSKFLKAGIGYGGS
                     CFPKDTKALHWLSEHNGYELKTVKAAIEVNESQKLKLVRKASKLVESFQDMKVAVLGL
                     TFKPDTDDIREAPSIPNIKYLLERGARVTVYDPIGVNNTKKIFGDSILYADSVDDAIE
                     DAEICFIMTEWKDVLNYDIHKYKEKMKNPLVLDGRNCYKVKDMEELGIEYYSIGR"
     gene            17769..18860
                     /locus_tag="KRL67_001142"
     CDS             17769..18860
                     /locus_tag="KRL67_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HBH1522041.1"
                     /translation="MKTVCYLADGSNPHTLKWCSFFKNKGYDIHVISLNGGHMEGIKI
                     YNFGSNVEELKNENISKKTGYLGSIKQIKKLVNEIKPDILHAQYASSYGFIGSLLGYH
                     PYIISVWGTDIYDFPNNGFIQKQIIKHNFRKADYIFSNSRDMAKEANKYTAKHVDVTF
                     FGVDMDRFKPMEVEKEDAFVIGIIKSLEKKYGIEYLIQAFKMLKDEYKDKKIILKIGG
                     SGSQMDNLINLTKELGIENDVQFLGRISPENVSKTFNSFDVTVFPSLREGFGVAAIES
                     EACEVPVIVTNVGGHPESVWQNETGLIVEPKQPEEIKNAIIKLMENDELRLSMGKKGR
                     QFVRENYEVNLNFNDIEKIYDSIFDKYKK"
     gene            18944..20857
                     /locus_tag="KRL67_001143"
     CDS             18944..20857
                     /locus_tag="KRL67_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HBH1522042.1"
                     /translation="MLESIDLEKESFFNRLFKKYKFRHLTLLILDICSVLVAFRMSLS
                     LTGNLNAFITNDIIISACVYIVLHIVSFRLFKCYNTLWRYAGEEEIISIFMATLAYLI
                     PIYIINKLLGFDYPIMFYVLNTIFIIMFTSGARIAYRAIRIVMNKTYSRGKVSNILII
                     GAGDAGEMVIQELKRNSELKKVAVAIIDDDKNKIGRIIHNVKIVGTTSDIKDVVEKYN
                     VDEIIFSIANIEKRRKKEIIDICKNTNCKIKTIPGIYEIIDGKVDIKQIREVEIEDLL
                     GREPIKTNLREISNYIEGKVILITGGGGSIGSELCRQIAGFNPKELIIVDNYENNAYS
                     IQQELLRKYKNKLDLKTVIASIREEKRMDEIFNKYKPEVVFHAAAHKHVPLMESSPGE
                     AIKNNIFGTLNIAGLSSKYRAKKFVLISTDKAVNPTNIMGATKRAAEMIIQTMNAESQ
                     TEFVAVRFGNVLGSNGSVIPLFKKQIEDGGPVTVTHPDIIRYFMTIPEAVGLVIQAGA
                     MAKGGEIFVLDMGEPVRILDLAKNLIKFSGFEPDVDIKIEFSGLRPGEKLYEELLMSE
                     EGLLDTEHKKIFIGRPIDVDREKITKYLKLLREITNNEEVEKIDGIMRELVPTYIKPE
                     DANIKEIATTREK"
     gene            21056..21763
                     /locus_tag="KRL67_001144"
     CDS             21056..21763
                     /locus_tag="KRL67_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HBH1522043.1"
                     /translation="MMNIKNKKHILKKFIAMVLIAGVVTVEAGAITASAAEPTNSPMS
                     ATVDQCDFLNVRSGASANDAVVGKINTGDKVEVLELHSNGWIKIKSVDNVTGWVNGDY
                     LTIQGGNVDAKVQNVLNLAFKQQGKPYRWGATGPNAFDCSGFTSYVYKNGAGVNLPRV
                     SRSQATVGKKVSRAELKPGDLVFFGSGGSINHVGLYVGDSKFIHSPQTGDVVKVTSMA
                     PGTNYARRLITATRVLQ"
     gene            22065..24176
                     /locus_tag="KRL67_001145"
     CDS             22065..24176
                     /locus_tag="KRL67_001145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding glycosyl-hydrolase Cwp19"
                     /protein_id="HBH1522044.1"
                     /translation="MKKISILVLSLIMTLTMCSVSSFADSSNDKEMRAAWISTVYNLD
                     WPKTKNNEAKQKKEYTDLLDKLKSVGINTAVVQVRPKSDALYKSNINPWSEYLTGTQG
                     KDPGYDPLPFLIEEAHKRGMEFHAWFNPYRITMADESIDKLPANHPAKKNPSWVVKHG
                     NKYYYDPGLPEVRKYIVDSIAEVVKNYDIDGVHFDDYFYPGVSFNDTATYQKYGKGQN
                     KDNWRRENVNTLLRDVKASIKSIKPNVVFGVSPAGIWRNKSSDPTGSDTSGNESYVGT
                     YADTRAWIKQGLIDYVVPQLYWPIGLKAADYSKLVAWWANEVKGTNVDLYIGQGIYKQ
                     GQSSYGGQNIAKEIVQQVTLNRKYSEIKGSMYFSAKDIANSTSIQKDLKSLYSSSEEP
                     VTPPSNVKVEKLRGDERYDTAVAISKKGWATNSDTVVLVNGYSIVDGITSTPLATSND
                     APILLVNKDNIPTSTKNELKRLNPSKVILIGGNNSIGDKVESEIKDTLSNVSINRVGG
                     SDRYSTSLMIAKELVKTNPVEKLYITSGTGEADSLSIASKAGEEKQPIVLVSKDNVSD
                     EVYNWISDLKVKDAYFIGGNLSISDSVINKLDKVITNDVSKNRIAGENREETNGKVIQ
                     KFYPNAEYSSMFVSKSNQLVDALTSGPLAAKLKSPVVMLGNSVTSAQKTALEPKKTTL
                     VYEAGDGINQNTLNTFLNLVK"
     gene            complement(24374..25339)
                     /locus_tag="KRL67_001146"
     CDS             complement(24374..25339)
                     /locus_tag="KRL67_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HBH1522045.1"
                     /translation="MSKLKKFVILLAFLVVIFPISVYGYFYYKLSAIHDSSISSDLLD
                     NNDHKNEDGIINILLMGTDARPNEDSSRSDAMMILTIDNKHNDIKLTSLARDSYVDIP
                     GHGKQKLTHAYAYGQADLLIQTIEENFNIDIQNYACVNFESFMYIIDAIGGVEVTIEK
                     GEIRELNKFIPETYKWNKSDDKGSIQYIRNAGKQTLNGYQALSFARIRHNDTAFARDG
                     RQRQIIQAIIKKTETLPVTKYPGLLDAVLPYVKTNMKPNAILSLGAQVLKMGDLNIKQ
                     FEFPIDDEIHSTGGIYGKAGWVLRFDPDTLDILHDFIFNDIEFKQ"
     gene            complement(25624..26580)
                     /locus_tag="KRL67_001147"
     CDS             complement(25624..26580)
                     /locus_tag="KRL67_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HBH1522046.1"
                     /translation="MSGLKKFVIFLALLVIAFPVSIYGYVFTKLKTIQDDSINTEALS
                     SNDYKNEENITNILLLGTDARPGEEVSRSDAMMILTIDNKNKSLKLTSLNRDTYVNIP
                     GRGEQKLTHAYVYGQANLLIETIEENFQLDIQNYAVVDFFSFMDIVDALGGVTVTVDR
                     SEIRELNKFIPETYKWNKNDNKGSLKYIRNSGEQKLNGYQTLAFSRIRKNDSTDERDR
                     RQRSVIQSLINGVKDLPVTKYPNLVNTILPYVKTNMNPNEIISLGKELLSIGNLNLKS
                     MEFPLSTENGRKIGNAGYVIPFEEYELDAMHDFIFKDIMVED"
     gene            26948..27736
                     /locus_tag="KRL67_001148"
                     /pseudo
     CDS             26948..27736
                     /locus_tag="KRL67_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893724.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
     gene            complement(27858..28328)
                     /locus_tag="KRL67_001149"
     CDS             complement(27858..28328)
                     /locus_tag="KRL67_001149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4358 domain-containing protein"
                     /protein_id="HBH1522047.1"
                     /translation="MKKILILFFSIILSLGMVSCSSSGDYKNVSTSKIKKALKSSDLL
                     IKESKIFDAKDFNYFNDVKEDIIQGFVINSSEKLSLEDIIVIKTDEVDKIYKTLENYK
                     KDMIQAPFGEGHGEKINSEIANNTIIEKAGRYVYLISAENATQIENKILDIIKK"
     gene            28467..29111
                     /gene="uppS"
                     /locus_tag="KRL67_001150"
     CDS             28467..29111
                     /gene="uppS"
                     /locus_tag="KRL67_001150"
                     /EC_number="2.5.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="di-trans,poly-cis-decaprenylcistransferase"
                     /protein_id="HBH1522048.1"
                     /translation="MRIPKHVGIIPDGNRRWASQNGMTKDKGYESGIDPGLESYRLCR
                     DLGCEEVTFYGFTTDNTRRPSVQTKAFTKACIDAANILIDEGAALLVVGNTKSPMFPE
                     ELLPYTNRIKAKEGQIKANLLVNYGWYWDLNNISGVENVNKNNIQDYIQSFDVSRLDL
                     IIRWGGRRRLSGFLPVQSIYADFYVIDNYWPDFKSDDIKDAFSWYDTQDVTLGG"
     gene            29154..30059
                     /locus_tag="KRL67_001151"
     CDS             29154..30059
                     /locus_tag="KRL67_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HBH1522049.1"
                     /translation="MKKKLLDGKITLIICKSVKIYTKKGEEMSKGNNNNNSRNKSKKT
                     SHLNRKKRKLNKKKLAVLICFTVLFLFIAFKATQGVVALVKSMDKSNKTSQQQNVNSE
                     QFDFGNEEENKKKKYTVFIDPGHGGNDKGTESKTSNRYEKDLNLQIAKKLANKLSKQK
                     DIQVVVSRTDDTYISLKDRAILANNSSADVLVSIHLNAEKNGNTATGIETWYRNKATD
                     GSKELAQAVQSTIVSYVKVRDRGIVENNFEVLRESNMPAILIECGFLTTPSEEQKIIN
                     EKYQDQLAEGIVQGVLSYLDSKGNK"
BASE COUNT        12059 a         3295 c         5407 g         9681 t
ORIGIN      
        1 aatttcaaat aatgctgtag attctaaact tatgaatagt aggattaaat caatagatat
       61 aataagaggg ctatcaatag ccctcatgat agtctgcaat aatcctggga cttggatgag
      121 aatgtatcct caattaagac atgctgtatg gcatggtgta acacttgcag attttgcatt
      181 tccttttttt gttatatctt taggtgttac aattccaata tcaataaata gtaagataaa
      241 aaataataaa tccacactga gtataatact tagtatattc aagagaagta tcttattgat
      301 attatttgga tttttcttaa attacctagg aaatcctgat ttagatactg taagaatact
      361 tggagtactt caacgtatgg gattagtcta ttttgtgact agtttagtat atttactact
      421 aaaaaaatta aatgttggaa gtactgctac aataattact ttcttatgta tatctacatt
      481 tataatagtt gggtactaca tattagcaaa accttatgga tttgagctag aaggttctct
      541 tgcacagtta gtggatttac atttcttcaa aggacatttg tacaagccgg aatttgaacc
      601 agatggtttt ttaactagta tagtagcaat ttcttcaggt atgttgggat gcactatggg
      661 atgtgtactt ttaaaagaag acataggaga atataaaaaa ttctttaaaa tactggttat
      721 gagcataatc ctactaatag gtgcttttat ctttaatcag tattttccat ttaataaaag
      781 attatggtct agctcatttg tacttttaat ggctggcagt tatggtatat tactgtcaat
      841 attttatttt atatgcgaca tcaaaaataa aagtaaaata tttaccccaa taatagcact
      901 tgggagtagc cctattttta catatatgtg ccttgagata ttgagtcatg ttttttggaa
      961 tgttccaaaa cttacgaaca aagtcgatta tccaactacc ttggtagagt ggactacata
     1021 tgaacttata acaccttggg caggtacaac ttgggattct cttgtatttt ctttattgta
     1081 tgtactattc tgggttatag taatgtctat aatgtacaag aaaaaaatat ttattaaaat
     1141 ttagtgcaat atttttagac gctaaagtct ttgatttgtt caaatataca gaaactatca
     1201 aatataatca aaatttctcc tcgataaaat ttaaaaacca tgccataaaa gattacaaaa
     1261 agtttacaat ttgtaatttt ttgtgctata atgaaaaaag atttacataa tattgaattt
     1321 atgtaatata aaacaagctt aagatgtatt aaaaaagatt taaagagtta aagaaccaaa
     1381 aaattagggg ggatatgatg aaaaaaacaa caaaattatt ggcaacagga atgctgtctg
     1441 tggcaatggt ggcgccaaac gtagctttag cggctgaaaa tactactgca aatacagaat
     1501 ctaattcaga tattaatata aatttacaaa gaaaaagtgt agtgttagga agtaaatcta
     1561 atgcaagtgt aaaatttaaa gaaaaattaa atgctgattc aataacatta aattttatgt
     1621 gttatgatat gccacttgaa gctactttaa attacaatga aaagacagat tcatatgaag
     1681 gtgtaataaa ctataacaaa gacccagaat acttaaatgt atgggaactt caaagtataa
     1741 aaataaatgg taaagatgaa cagaaagttt taaacaaaga agacttagag tctatgggat
     1801 taaatcttaa ggattatgat gttacacaag agtttataat aagtgatgca aattcaacaa
     1861 aagcagtaaa tgaatatatg agaaaaactt ctgcaccagt aaaaaaactt gctggagcta
     1921 ctagatttga aactgctgtt gagataagta aacagggttg gaaagatggg tctagtaagg
     1981 ttgtaatagt taatggagaa ttagcagcag atggaattac agctactcca cttgcatcta
     2041 catatgatgc gccaatatta cttgcaaata aagatgatat accagaaagt acaaaagcag
     2101 agttaaaacg tttaaatcca agtgatgtaa taattatagg tgatgatggt tcagtttcac
     2161 aaaaggcagt gtctcaaatt aaatctgctg taaatgtaaa tgtaactcgt attggtggag
     2221 ttgatagaca tgaaacatct ttacttatag caaaagaaat agataagtat catgatgtta
     2281 ataaaatata catagcaaat ggatatgctg gtgaatatga tgctcttaat atttcatcaa
     2341 aagctggtga agaccaacaa cctataatat tagctaataa agactctgta ccacaaaata
     2401 catataattg gctatcttca caaggtttag aagaagctta ttacataggt ggtagtcaaa
     2461 gtttatcatc aaaaattata gaccaaatat ctaaaatagc aaaaaatggg acttcaaaaa
     2521 atagagtttc tggagcagat agacatgaaa caaatgcaaa tgtaattaag actttctatc
     2581 cagataaaga gttgagtgca atgttagttg caaaatctga cataatagtt gactcaataa
     2641 cagcaggacc tttagcagct aaattaaaag ctccaatact tataactcca aagacttatg
     2701 tttctgcata ccacaataca aatttatcag aaaaaacagc aggaacagta tatcaaattg
     2761 gtgatggaat gaaagatagt gttataaatt ctatagcatc tagtttatca aaacacaatg
     2821 caccaatagg accagataat tcgggttcag cagcaggaaa aactgtagtg atagacccag
     2881 gtcatggggg ttctgattca ggggcaacaa gtggattaaa tggtggagca caagaaaaga
     2941 aatatactct taatacagca ttagcaacta ctgaatattt acgttcaaaa ggtataaatg
     3001 ttgttatgac tagagataca gataaaacaa tggctttagg agaaagaaca gcattatcaa
     3061 atactataaa gccagattta tttacaagta tacattacaa tgcgtctaat ggagctggta
     3121 atggtgtgga aatttattac aaagtaaaag ataaaaatgg tggaacaact aagactgcag
     3181 catcaaatat cttaaaaaga atactagaaa aatttaacat gaaaaataga ggaatcaaga
     3241 caagaacact tgataatgga aaagattacc tatatgtgtt aagaaccaat aattatccag
     3301 cagtacttgt tgaatgtgca tttattgaca ataagagcga tatggataag ttaaatactg
     3361 cagaaaaagt aaaaacaatg ggaactcaaa ttggaatagg aatagaagat acagtaaaat
     3421 aaaataaaaa atattaaagc aaaacatatc atctcctgtt ataatgtaaa atataacaag
     3481 agatgatata tttttgtaaa gaatcaaatt gtttacaatg atgtttcttg tattgataat
     3541 ctgctcatta ttgagtataa tatagaaata agtcaaaaat aagatagaaa taagttgaaa
     3601 ttaagaatta aaaaaatttt tattccaata tggaggtaac tatgtctgga tatactaatg
     3661 atgagtgtga aatacctaaa attaaaagct atccaggggc agataaagaa attgcaagtg
     3721 agattgacta tagcatagta aaaggaacgg ttttattcga cttgtaccaa agaattatgg
     3781 atttagtttt atctataata ggacttgtga taggtttacc tttaatagca atatttggaa
     3841 ttcttattaa aatagaggac aaaggaccaa taacttataa gcaagaaaga ctaggaaaat
     3901 gcgggagaag attctatata tataaattaa gatctatgag aacagacgca gaaaaatttg
     3961 gcgcacaatg ggctgaaaaa gatgatccta gaataactaa ggttggaaag tttattagga
     4021 aaactagaat tgatgagatt ccacagctgt ttaatatatt aaagggggac atgggcttaa
     4081 taggccctag accagagaga ccgaacttta ctgttcaatt taatgaggaa atacctggat
     4141 ttatcaacag gctcgctata aaacctgggc ttacaggttg ggcacaagta aatggtggat
     4201 acgaaatcac tcctgaagaa aaacttaaag aagatattta ctatataaaa aataggagta
     4261 tattacttga ctttaagata ctctttaaaa ctgttaaagt agtattgaca ggagacggag
     4321 ctagataatt ttaaaatatt agattactaa tcaatgaaaa agctctttaa aaagagcttt
     4381 tttattgtca aaaaatacaa aaaagaaaag gaatgagaac ttatgattaa aaaaatttct
     4441 actatattat cgttagtttt gttaatctct atttctagta cgataggagt atttgcagat
     4501 gcaaatccca aaagagagct gatagaaggg agtataccag agatttcgac agaattaaat
     4561 aaaagagcat ttaaggattc taaagaagta attcttgtaa atgaagagtc aattgtagat
     4621 agtataagtg caacaccttt ggcatatgca aaaaatgctc caatagttgt aactaagagt
     4681 aaaaatcttg gtagggtaac aagaaattat ctaaaagagc taggtccaga gaaggttacg
     4741 atagtaggtg gtttaaaggc agtatctaaa gatgctgaac gtaatataga aaaaatggga
     4801 ataaaagtag aaagaataag agggaaagac agatatgata catctttaaa gatagctaga
     4861 gaaatgtata gaacagtagg atttgatgaa gcatttttac ttagctcaac aacaggatta
     4921 gagaacgcca tatctgtata ttcttatgcc gctaaaagtg gaatgccaat aatatgggct
     4981 aaagatgaag attttgaaga acaaattgat ttcttaaaag gcaaaaatct taaaaaaata
     5041 tatgcattag gtgattcaaa agaatttatt gcagagattg attctaactt aaaaaatata
     5101 gaaggaataa agcaaataaa taagtcaagt acaaatgttg atttaataaa gaagttttat
     5161 gatgaaaaag atataaagaa aatctatact gctaaattag actttggtag cagatctgat
     5221 gtaaacgaat atatttcttt gggagtagta tcagcaaaag aaaatatgcc aatactgatt
     5281 tgtagtgata acttaagtcg tgcacaagat aaattcttga aagatagtaa tataaatgat
     5341 gtagttgaag ttgggtatac agtaggtgat tatagtctat ttaagagcat atttaatcta
     5401 acattcttat cttgtatagt attaatacta ttattactat tgattacatt tagggcactt
     5461 agatatgagt cgaaatagtt tttgatttat aaggaagctt ttcataggtt aaatttactt
     5521 aattagaata atgtttataa gcagttttaa aagttctact tttagagttt aaattagata
     5581 aggagaaaga agatgtcaaa aacagctaaa gcagcattgt ggattatggc agccactatg
     5641 ttttcaaaag tattaggttt tcttagagaa cttgtacttg caaatttcta tgggacaggt
     5701 atgtatgcag acgtatttgt attaacatta aatatacctg gtttaatcat agcagtcata
     5761 ggctctgcag ttgctacaac ttatatacct atgtactttg agacaaagaa aagactcgga
     5821 gatgaaggtg ccttaaaatt tactaataat gttttaaata tatgctatat aatggctata
     5881 gtaatagcta ttattgggct tttatttaca gagcaatttg ttacaatatt tgcagcagga
     5941 tttagaaacg accctgctaa gtttcaagca gcaatattat ttactaaaat aatgatttca
     6001 ggagttttat tcctgagtgg aagtaagata tttagttcgt ttttacaggt gaatgacagt
     6061 tttgtaattc ctgggcttat aggaatccca tataatatta taataatagc agcaatagca
     6121 ctaagtgctg gtaaaaatgt atggatactg ccagcagggg cactattggc tatggcgagt
     6181 caactattat ttcaactgcc atttgcattt aagaagtcct acaagtataa gccatatata
     6241 aatttaaagg atgagtccat aaaagaatta gtcaacctag tattgcctat gttggtagga
     6301 gtagctgttg gacagttaaa tatatttgtt gatagattat tggcaactac tttgggtgat
     6361 ggaaagttgt ctgctcttaa ctatgcaaat aggctaaatg agtttgttat ggcattattt
     6421 gttacatcta taataacagt tatatatcct aaactagcaa aaatgtctgg taaagataat
     6481 aaagaaggat ttataagtac tattgtaaaa tcttcaaatt gtattatatt ggtggttctt
     6541 cctatatcta taggagctat tatattagca gagcctcttg ttaggatatt attccaaagg
     6601 ggtaaatttg atgcgttatc aacagacctt acatctatag cgcttagatt atattcacta
     6661 ggtttattgg cttgtggagt aagagatgtc ttatataggg cattttactc tctttcagat
     6721 acaaaaacgc caatgataaa tggaagtata gctttaataa taaatatagt tcttaattta
     6781 atattgataa gaccattggg tcatgcaggt attgctatat caactagtac atccaacata
     6841 attacagtta tattattatt tatatctctt aaaaagaaaa atggatattt cggaggggat
     6901 aaaattataa agactggtct aaaaagtttg gtagcatctg gtgtgatggc agtagctact
     6961 ttattgatat ataataatct atatgcattt atgggttcag gaacaattaa agaaattata
     7021 tcagttggag ctggagtatt aggtggagct tctgtatata ccgtattgat agttttattt
     7081 aaggtagaag agatggattt agcttttgaa tttttaagaa aaggaaaaca aaaactactt
     7141 agaagaagat aattaccttg aaattttaaa ttagagtcag aaaggatgaa aatatggatt
     7201 ataagaataa ttatgagatg tggttaaatt ctccttattt tgatgaacaa acgaaaaatg
     7261 agctattaag tataaaagat gatgaaaaag aaatccaaga tagattttat aaaaatttag
     7321 agtttggaac aggtggttta agaggtataa taggagcggg aactaataga atcaatattt
     7381 atactgttag acgtgcaaca ctgggcgttt taaattatat catgaaaact caaggagaag
     7441 aaggtaaaca aaagggaata gttatagcac atgatagtag atacatgtct agggagtttt
     7501 gtatagaagt agcaaagact ttaagtgctt atggtgtaaa ggcttatatt tttgaagaat
     7561 taaagcctac accagaactt tcttttgctg ttagatattt aaagtgtgct atgggaatag
     7621 taataacagc aagtcataat cctaaagaat acaatggata taaagtatat gactcagatg
     7681 gtggtcaaat ctgtatagat atggcaaatg atataatagc agaagttaat aagattgatg
     7741 attacagtac tattaaaagt atagattttg aagaagcttt atctaaaaat ttaataacta
     7801 tattagataa tgaagtagat gatgagttta taaaagctgt taaaaagcaa gttttaagac
     7861 aaaatataat agatgaatac gggaaaaaat taaaaataat atatacgcct attcatggta
     7921 caggtaataa gtcagtaaga agagtcttaa gtgaatgtgg ttttaaaaat gtgatggtag
     7981 ttaaggagca agaattgcca gattctaatt tttcaacagt aaagtatcct aatcctgaag
     8041 aaaagtcagt gtttaatata gctattgaaa tggcaaagaa taatggcact gatttgataa
     8101 taggaacaga ccctgattgt gatagagtag gtatcgttgt aaaagattca agtggtgaat
     8161 atgtagtctt aaatggaaat caagtcggtt cacttttagt tagatatata ttagagagtt
     8221 tagttgaaga aaataaactt cctaaaaaca atcctacaat aataaaaact atagttacat
     8281 cagaactagg tgcaaaaata gcaaaggctt ataatgtgga ttgtttaaat actttaacag
     8341 gatttaaatt tataggagaa aaaataaaag cgtttgaaga aagcaatgat agaagtttta
     8401 taatgggata tgaggaaagc tatggttact taattggaac tcatgctaga gataaagatg
     8461 gtgttgtatc ttcacttatg atatgtgaaa tggcagcata ctattcttca aaaggtatga
     8521 acttgtatga agctttgata gatacatata ataaatttgg atactacaaa gaggacttaa
     8581 agtcagtaac tttaaaaggc atagatggaa taaaaaaaat aaaagagatg atgctttact
     8641 ttagaagtgt taaaatagat aatgtagctg atgtaaaagt agataaaatt ttagactata
     8701 aagatggtgt tgatgattta cctaagtcgg atgtacttaa atttttactt gaagatggct
     8761 cttggatagc tataagacct tcaggtacag aacctaaaat taaattttac tttggagcaa
     8821 attcagacaa tcaagaagat gtagaattta aattgaacaa tttgatatct tatatattaa
     8881 atgtagtgga ttctatataa aaagctaagg ggagataaat aatgaaagta tacaatgtca
     8941 taatggctgg aggaggagga actcgtttct ggccacttag tagacaagaa gtacctaaac
     9001 agcttataaa tttaagtgga gaagatgctt taataaacga aactataaac agaatagatt
     9061 ctctagcaaa aaaagatgat ttatttatag taaccaatga gaagcaattg gaagctttaa
     9121 aggatatagt aaaagataaa tgtttggata gtaatatatt gccagagcca tgtgccagaa
     9181 atacagcagc agctataggc tttgcagcat ttaacataat gaaaaaatat ggtgatggtg
     9241 ttatgtgtgt atatccagct gaccattata taaaagatga aaaagaattt aaatctattt
     9301 tggaaaaagc tatctatata gcagaaaata atgataaact agtaactatt gggataactc
     9361 ctacatttcc atcaacagga tatggataca taaattttaa tagagaaaat acgatagaag
     9421 atgttgcata tgaggtagtg gagtttgtag aaaaaccaaa ctatgaaatt gctaaagaat
     9481 atgtaaactc taaaaagtat gtatggaata gtggaatgtt cgtatggaag gtatctaaaa
     9541 tattagaaga ttttaagaga tacttgccaa aagtatatga aaaattagaa gatataagta
     9601 aatacttagg aacaaaagaa gaaatggaaa aaataaaaga aatataccca actatacaat
     9661 caatctctat agattatggt attatggaaa gaagtaatga tgttatagta gttcctggtg
     9721 attttggatg gaatgatgtt ggttcttggg attctctagg tgcaatatat ccaactgatg
     9781 atgaaggtaa tatcaaaaga ggtgaaaata ttactataga tactaaaaat tcaattatat
     9841 attcagatga caaattaata tcgacaatag gtattagtga tttgatagta gtatctacaa
     9901 atgatgcagt tatggtttgt agaaaagata aagctcaaga tgtcaaaaaa atagtagagc
     9961 agttaaaaga agaagataga caagaatata tgtaggaggt gttcatttga ttcctaaaaa
    10021 aatacattat gtatggtttg gtggtccaaa aggaaatata gaaaatatat gtataaattc
    10081 ttggaaagaa aagcttccag aatatgagat agtagagtgg aatgaaaaaa actttgatat
    10141 agagaaagaa ataaaaggaa ataagttttt ggaagaatgt tataaaagaa aactttgggc
    10201 attcatatct gattatacaa gaataaaagt actctatgaa caaggtggag tttatatgga
    10261 tacagatatg caaatattaa aggatataac tcctctttta gaaaataata ggctaatctg
    10321 tgggtatgaa gatgatagag agtatataaa tggtgctata ataggtgtag aaaaaggaca
    10381 tcctttttta aaagacttat tggagtatta tgaaaaagaa gtacttactt cttcattgtt
    10441 tacaatacca aagataatga ctcatcttat ggaaaagaat tataagaaga ttgacccaaa
    10501 taattatgaa gaaggaatac gtgtttatga caaagaatat ttttatcctt ttggatttaa
    10561 agaagacttt acaccagaat gtataacaga aaacactttt gggatacatt ggtggggaaa
    10621 aagttgggct aaaaagagaa attatttctt agaaagcaaa cacttaacag gtgtaaataa
    10681 gatatggaag tgttgtaaaa tatttgcaag taatacttta cgaagctaaa agggagaaag
    10741 taaaatgaag actttaaatg atgatagaaa tattatgatt agaaaatatc tttatatatt
    10801 tgttttgttt atcatattac ttagaaagta tatgatatca tttattgacc caaatatgga
    10861 tatagggatg attaaatcag cgttatttta ttcttcaata ggaatactaa tgctactttt
    10921 tttatttgat aagagaaaaa gtataacaga aatggtacta gttggagttt gtgtcttact
    10981 ttatttatta aatagggaag gagctatatt attaatcgtt ttattagctg tatcagctaa
    11041 gcaaatagat gataaattta tagttaaaaa ttatttaata atatcggcat gttttttaat
    11101 ggtagctata ttattattta atttatttcc ttcattaata tttaatcagg aagtccacta
    11161 tagatatata gaaaaaatag atatgcttgt tacaagaatg gattttggac ttggtaatcc
    11221 aaatagtgta ttttatcata tggttactat atacgcagca tatatattct taagatataa
    11281 agattataat aaatgggata gaataatttt atttggttct gcattttttg tttatcaaac
    11341 cacatatagt agaactggat ttttcactat attagcagga ttaatatttg tagaaataat
    11401 aagatggata gatataaaaa aaataaaagg actaccaatg cttctaaaga cattgccgat
    11461 aatacttact ttatttagtg taattattgg tacagtattt gataaaagca ctttattaaa
    11521 tagattatta gcaagtagac ctaagttctg gcatgtatat ttggctgaaa aggggaactt
    11581 tctaaaacct tttggaaatt catatagccc tgctataaaa gcaactaatc cattagatag
    11641 ttcttatgta tatataatta gtgtactagg agtagttgct tgtatattat ttatgtattt
    11701 gatgtataaa ggaatagaaa gctttataga aaaagataaa aaagcatatt tagtagcttt
    11761 atttatattt ttactatact cttttgcaga aaatttatta ttagaagctt catttagttt
    11821 tgcagtagta ttactaataa aagaagtgat attaaatgat aaaagggaaa tagatttgtg
    11881 gaagatgaaa agtaggaggt agtaatgtta atatcattaa taatgccaac attaaataga
    11941 tatgatgata tatacttact tatggacagc ttagaaaatc aaacttacaa aaactttgaa
    12001 cttatagtag tagaccaaaa tgataatagc aaagttaaag aaatagttga taaatacatt
    12061 gataagttag atataaagta tataaaaagt tctaaaaaag gattgagtta taaccgaaat
    12121 gtaggcatag acaatgctgt agggcaaatt ataggtttcc ctgatgatga ttgtgtctac
    12181 gaaaatgata cattagaaaa agttataaat ttctttaata aaaataaaga ttataaaata
    12241 tacagttgca aaactatgga ttcaaataaa gttgatgcat ttaagaaaat gtatgatgga
    12301 acttgtgata tcacaagttc caacgttttg gatactataa catccataac tttttttatt
    12361 gattttgaag gtaaggatta tacaagattt gatgaaaaat tgggtgttgg tggagagttt
    12421 ggtgctggtg aagagataga ttatgtttta aacttattga gtttaggttt taaaggaaaa
    12481 tactttggaa atgatataat ttaccatcct gcaaaaaaac attctaaatc taaagaaaag
    12541 tatcaaaaag attataacta tggaagaggc tttggagcgc tttgcaaaaa ggaaatagtc
    12601 tatagaaaaa attataagtt tgcaaaagtt atggtatcaa aacttgtaag aaatatagga
    12661 ggtcttatat tgagttccaa tagagattat catagtgcca ctataaaagg aagaattaat
    12721 ggatttagac agtataagtt ataatgagga tggtgtttat ggacaaggtt aaatttttaa
    12781 taaatatgct tttggctttt tttatatatc catttaataa gcataagttt aatggtagaa
    12841 atatctggct tataggtgga cattcagggg atatatacaa tgataattca aagttttttt
    12901 atgagtatat gctaaaagaa cataatgatg tagaaactta ttgggtagta aataaagaca
    12961 gtaaagtatt tgataaaatt ccaggcaaaa aattaataag aggaagtgta gaaaactatc
    13021 tgtattatta caattcaaaa gctatagtat tttcacatgc accatcagca gatatagcac
    13081 catacaattt tgctgtacct gttcttaatt attttcataa aaaaacgata aaagtgtttt
    13141 taaatcatgg gactataagt tttaaaaaga gaaaacctat gaacaaaaag ttcaaaaaca
    13201 ttatagataa cctatataaa agctataata tagttacagc aagttctgaa tttgaaagaa
    13261 atgttatggt aaatgattgg ggaatgttag atgactcagt ttatataatt ggaaatgcaa
    13321 gatatgacaa tttaccaaca aatgaagttg cacaaactcg agatatatta tatactccta
    13381 catggagaga ttggataaaa tttagttcag gaaaatttac ggatacagat tattttaaaa
    13441 atataatgaa ttttttaaat gatgataaat taaataaaat attagatgag aaagatataa
    13501 atgtaaaaat ttatatgcat catcttatgc atgagtttat agatgatata aaagaaaata
    13561 taacaggaaa acgaattgtg tttttagata aaggggtaac tctagcaaat gaaattagaa
    13621 aatcagctgc aaatataact gattattcaa gtgtagccat agactttttg tatatgaata
    13681 gacctatctt gttttaccaa ttcgatttgg atgaatatat ggaaaaagtt gattcatata
    13741 tagatttaaa aagtgaaatg tttgggtctt tggcttataa taatgatgaa gccgtgaata
    13801 aacttattga tattattgaa aataattttg aggttatgga taaccaaaaa aatgaaagaa
    13861 ataagttttt cagatataat gataataaga actgcaagag gatatatgat tgtgtattaa
    13921 gtaaaattaa ataaatattt tataaatgat gaggaaggta atatgaagaa gaatttagta
    13981 tctataatta ctcccatgta taattctgaa aaatttattg aagcaaccat aaaatcagta
    14041 ttaaaccaaa cttatcaaga atgggaaatg ttaattattg atgattgctc aacagataat
    14101 agtcctaata tagtcaaatc ttatatgcaa caggatagta gaataaaatg tataaagact
    14161 gagactaata agggtgtctc taatgctaga aatttagcac taagtaaggc aacaggacaa
    14221 tttatagctt ttttagatag tgatgaccaa tggaatagta gtaagttaga aaaacaagta
    14281 aattttatgt tagaaaatga ctatgtaatt tcatttactt catatgaact gatggatgaa
    14341 aatgataaaa aattaaacaa agtaataaaa gttccaccta atgtagatta taaaagatta
    14401 ttaaaaggaa atatcttagg atgtcttact gttgtgattg ataagtctaa attggatttt
    14461 gaaataagaa tgagtggtgt aagacatgaa gattatgttt tatggttatc gatattgaaa
    14521 aaagggcata ttgctcatgg aataaatgaa gttctagcat tatatagaaa atctagcaat
    14581 tccctaagtg gtaataaaat aaaagctgct atgtggactt ggaatatata tagaaatata
    14641 gaaaaaatac ctttatacaa agctatatac tactttatta attatggaat aaacggaatc
    14701 aaaaaaagtt agacagagag gaagatttaa ttaatgtata aatataaatt tactgtattt
    14761 actccgacgt ataacagagc tcatttactt gaaaatttgt acaatgattt aaaagctcaa
    14821 acttatgatt tcaatgattt tgaatggttg attgttgatg atggaagtag cgacagtact
    14881 aaagagttag ttgaaaaatt tatatcagaa aataaattaa atataagata tatatacaaa
    14941 gaaaatggag gaaaacatac tgcaataaat ctaggtgtaa aaaatgcaga tggagagcta
    15001 tttttcattg tagatagtga tgatggttta attaaagatt ctcttaaaat tgctaatgat
    15061 gagtgggatt ctttagagaa taaagaagga tttagtggag ttgttggact ttgtgtatat
    15121 ccaagtggag atttgatagg gacagaaatg cctgaagata agaaaatatg tcactttgca
    15181 gacctatatt ttaagtatgg cgttaaggga gataaaagca tagtatttgt aactgatgag
    15241 cttataaaat ttccttttcc agaaagaaaa gaagtgagat ttttaccaga aagtgtagta
    15301 tggaatgaaa tgtctaaata ctataaagtt atatgtgtaa ataaacctat gataataaga
    15361 gattatttgg atgatggttt gactaagaat atattaagta aaaatgcatt aagaggtaga
    15421 gcattagagt ttttatattt aataaatcaa aatacatatc cattgagtag atatccatat
    15481 atgtggataa aaaattacat aaatttagcc agatactcat tgttgtctga tagtcattat
    15541 tttggtgaat taagaaaagt atctgataaa ttgttgtatt tagcattatt cccattagga
    15601 tattacaaat atattggaca aagaaaatta gtatctaagt aaacttgttg gtatagtact
    15661 gctagttgct atataaggag agtaaaaata tgaatgagcc tttagtatcg ataattactc
    15721 ctgtttataa ttcagaagag tttttatcag agacgataaa gtcaatccaa aatcaaactt
    15781 ataaaaattg gcagctttta ttagtggatg attgttctaa ggacaattca agtgaaatta
    15841 taaaaagttt tcgaaaggaa gatgctagaa taaaatatat aaagcttgaa aaaaattctg
    15901 gagctgcagt atctagaaat gttggtatta agaatgcgga aggtcgattc attgcatttg
    15961 tagatagtga tgatttatgg gatagtagaa aattagagat tcagatagaa tatatgttaa
    16021 aagaaaatgt aggtttttct tttacatctt atagatatat gagacaagat gggtcaaaaa
    16081 cgaataaggt tgcaagagct cctaagaaaa ttgactatga aggattactt agaaatacta
    16141 taataggatg ttctacagtg gtcattgata aggagattgt aggagagttc agcatgcctt
    16201 tggtaagaag aggacaagat actgcaacct ggttacagtt acttaaaaag gaaaaatatg
    16261 catatgggat tcaagaagat ttagtgaatt acagattagt tggaaattct atatcaagta
    16321 acaagataaa agcattaaaa cgaacttgga atacttatag aaatgttgaa aatttaagtt
    16381 taccaaaaag tctatatgta ttttgtttct atgtttttaa tgccattaaa aaaagagtat
    16441 aagttttata tacttggagg taaaaaaatg aaaatagcta tagctgggac gggatatgta
    16501 ggtttggtta caggtgtttg tctagcagag gtaggcaatg aaaatgtagt ctgtgttgat
    16561 gtagatgaac aaaaagttaa atcaatgaaa cttggaatag cacctatata tgaagaaggc
    16621 ctagaagaac ttatggttaa aaattacaac agaggaagaa tagattacac tacagactat
    16681 aaaaaagctt atgaagatgt agatgtaata ttaatagctg taggtacacc tgagagaagt
    16741 gatggctcag ccaacctaga ttatataaag gcagttgcaa agcaaatagc tgaaactgtg
    16801 acaaaagata ctctagtagt aataaaatct acagttccaa ttggtacaaa tgaagagata
    16861 gaagattata taaatagaaa cctaaaaaat gatgtaaaag tagaacttgc atctaatcca
    16921 gagtttttat cacaaggaac agcagtaaaa gatactctac atgcttcaag aatcgttata
    16981 ggtgtagaaa gtgaatggtc tagagaaata ttagaagaaa tctataagcc ttttaatcaa
    17041 cctataatag ttacaaatag aaatagtgct gagatgataa aatacgcatc aaataacttc
    17101 ttagctctta aaatatcatt tatgaatgat attgcaaatc tttgtgaaat agcaggggct
    17161 aacatagaag atgtagcttt aggaatgagt tatgatgata gaataggaag taagttttta
    17221 aaggcaggaa taggatatgg cggttcttgc tttccaaaag acacaaaagc tcttcactgg
    17281 ttatctgaac ataatggtta tgaattaaaa acagtaaaag ctgcaataga agttaatgaa
    17341 agtcaaaaac ttaaattagt tagaaaggca tctaagttag tagagagctt tcaagatatg
    17401 aaggttgctg ttttaggttt gacttttaaa ccagatactg atgatataag agaagcacca
    17461 tctataccaa atataaaata tttgctcgaa agaggggcaa gagttactgt atacgaccca
    17521 attggtgtaa ataatactaa gaaaatattt ggtgactcta tactttatgc tgattctgta
    17581 gatgatgcga tagaagatgc agagatatgt tttataatga cagaatggaa agatgtatta
    17641 aattatgata tacataagta taaggaaaag atgaaaaatc ctttagtcct tgatggaaga
    17701 aattgttata aagttaaaga tatggaagag ttaggaattg agtattattc gataggaagg
    17761 taaatattat gaagactgtt tgttatttag ctgatgggtc aaatcctcat actttaaaat
    17821 ggtgtagttt ttttaaaaat aaaggatatg acattcatgt aatctcttta aatggaggtc
    17881 acatggaagg aataaaaatt tataattttg gttctaatgt agaagagcta aaaaatgaaa
    17941 atatttctaa aaaaacggga tacttaggtt cgataaaaca gataaagaag ctagtaaatg
    18001 aaataaagcc agatatattg catgcacagt atgcaagtag ttatggattt ataggaagtc
    18061 tacttgggta tcatccatat attatttctg tttggggtac tgatatttat gatttcccta
    18121 ataatggttt tatacaaaaa caaattataa aacataactt tagaaaagca gactatatct
    18181 tttcaaatag tagagatatg gctaaagagg ctaataaata tacagctaaa catgtagatg
    18241 taaccttttt tggagtagat atggataggt ttaagcctat ggaagtagaa aaagaagacg
    18301 cttttgtaat aggaattata aagagtttag aaaaaaaata cggaatagaa tatttaatac
    18361 aagcatttaa gatgcttaaa gatgaatata aagataagaa aataattctt aaaataggtg
    18421 gctctggaag tcagatggac aacctaataa atttaactaa agaattgggt atagaaaatg
    18481 atgttcagtt tttaggaaga atatctccag aaaatgtaag taagactttt aattcttttg
    18541 atgtaactgt atttccttct ttaagagagg gatttggagt ggcagctata gaatcagaag
    18601 catgtgaagt acctgttata gttacaaatg taggaggaca tccagaatct gtatggcaaa
    18661 atgaaactgg cttaattgta gaaccaaaac aaccagaaga aataaaaaat gctataataa
    18721 aactaatgga aaatgatgaa cttagactta gtatgggtaa gaaagggcgt caatttgtta
    18781 gagaaaatta tgaggttaat ttaaacttta atgatataga aaaaatttat gattctatat
    18841 ttgataaata taaaaaataa aatgaaaaaa tacagcaaaa gatagatgtt gttggctatg
    18901 cttaggtgcg tagcttttgc tgtttaaagg agggcttcta gttatgctag aaagtataga
    18961 tttagagaaa gaaagctttt ttaataggct ttttaaaaaa tataaattta gacatttaac
    19021 actattaatt ctagatattt gtagcgtact agtagcgttt cgaatgtctt taagcttgac
    19081 aggtaattta aatgcattta tcactaatga tataataatt tcagcttgtg tatatatagt
    19141 acttcatata gtatcattta gattgtttaa gtgttacaac acattatgga gatatgcagg
    19201 agaagaagaa attatatcta tttttatggc aactttagct tatttaattc caatttatat
    19261 aataaataaa ttattagggt ttgattatcc tataatgttt tatgtattaa atacaatatt
    19321 tattataatg tttacaagtg gtgcaagaat agcctataga gctataagaa tagtcatgaa
    19381 caagacatat tctagaggaa aggtttcaaa tatccttata attggtgcag gagatgcagg
    19441 agaaatggtt atacaggaac taaaaagaaa ttcagaactt aagaaagtag cagtggctat
    19501 aattgatgat gataaaaata aaattggaag aatcatccat aatgtgaaaa ttgtaggaac
    19561 aacttcagat ataaaagatg tggtagaaaa gtacaatgta gatgagataa tcttttcaat
    19621 agcaaacata gagaaacgta gaaaaaaaga gattattgat atatgtaaaa acactaactg
    19681 taaaataaag acaataccag gaatttacga aattatagat ggaaaagttg atataaaaca
    19741 gattagagaa gtagagatag aagatttgtt gggcagagag cctataaaaa caaatttaag
    19801 agagataagt aattatattg agggaaaagt aatcctaata actggaggag gaggctcaat
    19861 tggttcagag ctttgcagac agattgcagg atttaatcct aaagagctta ttatagttga
    19921 taattatgaa aacaatgctt actcaataca acaagaatta cttagaaagt ataaaaataa
    19981 attagattta aagacagtaa tagcatcaat aagagaagaa aaaagaatgg atgaaatatt
    20041 taataaatat aaaccagaag tagtatttca tgcagcagca cataaacatg ttcctcttat
    20101 ggaatcgagc cctggcgaag ctataaagaa taatatattt ggaactttaa acatagctgg
    20161 cttgtcttca aaatatagag ccaagaaatt tgtacttata tctacagaca aggctgtaaa
    20221 ccctactaat ataatgggag ctactaaaag agctgctgaa atgattatac agactatgaa
    20281 tgctgaaagc cagactgaat ttgttgctgt tagatttgga aatgtacttg gtagtaatgg
    20341 tagtgtaatt ccacttttta agaagcaaat tgaagatggt ggaccagtga cagttactca
    20401 tccagatatc attagatatt ttatgacaat accagaagca gtaggcttag ttatacaagc
    20461 tggagctatg gctaaaggtg gagaaatatt tgtgcttgat atgggagaac ctgtaagaat
    20521 attggattta gcaaaaaatt taattaaatt cagtggattt gagccagatg ttgatataaa
    20581 aattgaattt tctggtctta gaccaggaga aaagttgtat gaagaactac ttatgtcaga
    20641 agaaggtctg ttggatacag agcataaaaa gatttttatt ggtagaccaa tagatgttga
    20701 tagagaaaaa ataacaaaat acttaaaact tttaagagaa attactaaca atgaagaggt
    20761 tgaaaaaata gatggtatta tgagggaatt agtgccaact tatattaaac cagaagatgc
    20821 taatatcaaa gaaattgcaa caactaggga aaagtagaat atatttctaa tttgtaacca
    20881 gggggagcaa aaagaaacaa atatatacta aaaagaaaca aaagattaca aaaagattac
    20941 aaaaagatta caaaaaaggt tgcaatttat tttgatgtaa gatagaatgt taattaggaa
    21001 ttggaaacga aggaacaaaa tgttaaattt tcgaaaggat taggtatttg agattatgat
    21061 gaatattaaa aataaaaaac atatattgaa aaaatttata gctatggtgt taatagcagg
    21121 agttgtcaca gtagaagcag gagcaataac ggctagtgca gcagagccaa ctaattcacc
    21181 tatgagtgca acagtagacc agtgtgattt tttaaatgta agaagtggag ctagtgcaaa
    21241 tgatgcagta gtaggaaaga taaacacagg agacaaagtt gaagttttag aattacattc
    21301 aaatggatgg attaaaataa aaagtgtcga caatgttaca ggatgggtaa atggagatta
    21361 tttaactatt caaggtggaa atgtagatgc aaaggtacaa aatgttttga acttagcatt
    21421 taagcaacaa ggtaagccat atagatgggg agcaactgga ccaaatgctt ttgattgttc
    21481 tgggttcact tcttatgtct ataaaaatgg agcaggagtt aatttaccaa gagtatcaag
    21541 atcacaagca acagtaggaa agaaagtatc aagagcagaa ttaaagccag gagatttagt
    21601 tttctttggt tcaggcggtt caataaatca cgttggattg tatgtagggg attctaaatt
    21661 catacattca ccacaaacag gagatgtagt aaaagttact agcatggctc caggaactaa
    21721 ttatgcaaga agacttataa ctgctacacg tgttttacaa taataataga tattgattta
    21781 cataggattg aatattaaca ttaaatatat aattacattt aatgttttac atagagattt
    21841 aagagtttag taatattaaa atagtgatga acgttataaa aacagcaaag aagataaatt
    21901 tctttgctgt tttttttata ataaattatt tcatattata atcaaatatc aaatataaat
    21961 tttaactata ttattcaata tttgacagat aatatgaatt ttagatatac ttaataagaa
    22021 gtatattgta gaaaaaataa atttaaaaat ttaaggggga tgaaatgaaa aaaataagta
    22081 tattagtatt atcattgata atgacactta ctatgtgtag tgtaagttca tttgctgatt
    22141 catcaaatga taaggaaatg agagctgctt ggatatctac agtttataat ttagactggc
    22201 ctaagactaa gaataatgaa gccaagcaaa aaaaggaata cacagattta ttggacaaat
    22261 taaaaagtgt aggaataaat acagcagtgg ttcaagtaag accaaaatca gatgctttat
    22321 ataaatcaaa tataaaccca tggtctgaat atcttactgg tacacaaggt aaagaccctg
    22381 gatatgaccc attaccattt ttaatagaag aagcacataa aagaggaatg gaatttcatg
    22441 catggtttaa tccatataga ataactatgg cagatgagag catagataaa ttgccagcaa
    22501 accatccagc aaagaaaaat ccaagctggg tagttaaaca tggaaataag tattactacg
    22561 acccaggttt accagaagtt agaaagtata tagtggatag tatagcagaa gtagttaaaa
    22621 attacgatat agatggagtt cattttgatg attacttcta tccaggagta agtttcaatg
    22681 atactgctac atatcaaaaa tatggaaaag gacaaaataa agataattgg agaagagaaa
    22741 atgtaaatac tctacttaga gatgtaaaag cgtctataaa gagtataaag cctaatgttg
    22801 tatttggtgt aagcccagca ggtatatgga gaaataagag tagtgaccca acaggttcag
    22861 atacatcagg taatgaaagt tatgtaggta catatgcaga tacaagagca tggataaaac
    22921 aaggattaat tgactacgta gttccacaac tttactggcc aattggtctt aaagcagcag
    22981 attattctaa attagtggca tggtgggcta atgaagtaaa aggaacaaat gtagacttat
    23041 acataggaca aggtatatat aagcaaggtc aatcgagtta tggtggacaa aatatagcaa
    23101 aagaaatagt acaacaagta acattaaata gaaagtatag tgaaataaaa ggtagtatgt
    23161 atttctcagc taaagatata gcaaatagta cttctataca aaaggattta aagagtttat
    23221 attctagtag tgaagaaccg gttactccac catctaatgt taaagtggag aaattaagag
    23281 gcgacgaaag atatgacaca gcagtagcaa taagtaaaaa aggttgggct acaaattcag
    23341 atacagtagt attagtaaat gggtactcta tagttgatgg tataacatca acaccacttg
    23401 ctacatcaaa tgatgcgcct atactattgg taaataagga taatatacct acatccacta
    23461 agaatgagtt aaaaagactt aatccaagta aagttatttt aattggagga aataactcta
    23521 ttggagataa agttgagagt gagataaaag atacattgtc aaatgtatca ataaatagag
    23581 ttggtggttc agatagatat agtacatctt taatgatagc aaaagaatta gtaaaaacta
    23641 atccagtaga aaaactatat ataacaagtg gtacaggtga agcagattct ctttctatag
    23701 cttcaaaagc aggagaagaa aagcaaccaa tagtattagt atcaaaagat aatgtaagtg
    23761 atgaagttta caattggata tcagatttaa aagtaaaaga tgcttatttt ataggtggta
    23821 atctaagtat atctgattca gtaataaata agctggataa ggtcattaca aatgatgtat
    23881 ctaaaaatag aatagctgga gaaaatagag aagaaacaaa tggaaaagta atacaaaaat
    23941 tctatccaaa tgctgaatat tcatcaatgt ttgtaagtaa aagtaatcaa ttggtggatg
    24001 cattaacatc aggtccttta gcagctaaat taaaatcacc agtagttatg cttggaaata
    24061 gtgtaacatc tgctcagaaa actgctttag agcctaagaa aactactcta gtatacgaag
    24121 ctggagatgg tataaatcaa aacacattaa atactttctt aaacttagtg aagtaataaa
    24181 aaagttaatt taaaatacag agtattttat aaatgtagat taaaatgaaa attatcagga
    24241 taaattagaa aataaaaaag aactatatgc attttgtaat caagtgtaca cagtaacttg
    24301 aaaataatta tgcgatatag ttctttttta gtaattaatc ttatactttt taataaattt
    24361 tatgttgaat tttttattgt ttaaactcta tgtcattaaa tataaaatcg tgtaatatgt
    24421 ctaaagtatc tgggtcaaat ctaagaaccc atccagcttt tccatatata ccacctgtgc
    24481 tatgaatttc atcatcaata ggaaattcaa attgcttaat atttaagtca cccattttta
    24541 aaacttgagc tcctaaagaa agtattgcat taggtttcat gttagtttta acgtatggaa
    24601 gaactgcatc taataaacct ggatatttag taacaggtaa agtttccgtt ttcttgatta
    24661 tagcttgtat aatctgtctc tgtctgccat ctctagcaaa ggctgtatcg ttgtgtctga
    24721 tacgtgcaaa agatagtgcc tgatatccat ttagagtctg ctttccagca tttctaatgt
    24781 attgtatgct acctttatca tcacttttat tccatttata agtttcagga ataaatttgt
    24841 ttagttctcg tatttctcct ttttcaatgg taacctcaac gccaccaata gcatcaatta
    24901 tatacataaa tgattcgaaa ttaacacaag cataattttg aatatcaata ttaaaattct
    24961 cttcaattgt ttgtattaat aaatcagctt gtccataagc ataagcatga gtaagttttt
    25021 gtttaccatg accagggatg tctacatagc tatcacgagc taatgatgta agtttgatat
    25081 cattgtgttt gttatcaata gtaagtatca tcatagcatc tgaccttgaa gaatcttcat
    25141 ttggtctagc atctgttccc ataagtagta tgttaattat cccatcttca tttttatggt
    25201 cattgttatc caataaatca ctacttatgc tcgagtcatg aatagcagat aatttataat
    25261 aaaaatatcc atagacgctt atagggaata ttactaccaa gaaagccagt agtataacaa
    25321 atttctttaa ttttgacaaa agtacccctt ctttcttctt tttcctgtat attttttaat
    25381 agcatacact taattatatt aaagtattga aatatattca atacttgttt cagttacaaa
    25441 aaaaagacaa tgatgtaatt tataagaaaa tttatcgact tatatacaca gttaaaagta
    25501 tatataatat attttttaaa agtaatttgt attttaaaat aaaaacacac cccctaaata
    25561 caaagtaatt tgcatttaag aggtatgtat ttatcatcaa attattagtt tattttggaa
    25621 tttctaatct tcaaccataa tatctttaaa tataaaatca tgcattgcat ctagttcata
    25681 ttcctcaaaa ggtattacat agcctgcatt acctattttt ctaccatttt cagtagaaag
    25741 aggaaattcc atagatttca aatttaaatt tcctatactt aaaagttctt ttcctaaaga
    25801 tattatttca ttaggattca tattagtttt tacatacgga agtattgtgt taactagatt
    25861 aggatattta gtgactggta aatccttaac accatttatt aaactctgta taacacttct
    25921 ttgtcttcta tctctttcat ctgtgctatc attttttctt atacgagaaa atgcaagtgt
    25981 ctgatatcca tttaattttt gctcaccaga atttcttata tatttaagag aacctttatt
    26041 gtcattttta ttccatttat aagtttcagg aataaattta tttaattctc ttatttcact
    26101 cctatcaaca gtaacagtaa caccacctaa agcatcaact atatccataa aagagaaaaa
    26161 gtcaactaca gcgtagtttt gtatatcaag ttggaagttt tcttctatag tttctattaa
    26221 taagttagct tgtccatata cataggcatg tgttaatttt tgttctccac gtcctggtat
    26281 attaacataa gtatctctat ttaatgaagt taatttaaga cttttatttt tattatcaat
    26341 agtaagtatc atcatggcat cagaacgaga aacttcttct cctggtcttg catctgtacc
    26401 taacaataat atgtttgtga tattttcttc gtttttatag tcattactac taagcgcctc
    26461 tgtatttata ctatcatctt gtatagtttt taatttagta aaaacatatc catatatact
    26521 aacaggaaat gctataacta aaagggctag aaatataacg aactttttga gtcctgacaa
    26581 taatatcccc tactttctaa attttttaat tacacatagt taattatatt aaaaatttgt
    26641 tacataagca ataaaaaaca caataaatta gcataagttt gtcataatat aattattttt
    26701 atagtatttt atagaagaaa gtgtgtagtt attattgatt ttattggaat ataattgtat
    26761 gaagacttaa atttttataa actgatgtgt atattgaaaa aaatattttc ctgaatgtag
    26821 ttatagcatc ttatgaaagg tgcaaattgt aatttaaaat ttttagtgta taataagaat
    26881 tgaaaattca aaccgataaa aattaataaa aaataattat aaattaataa tttgggggag
    26941 gaaatgtatg ataaaattaa tagcaacaga tttagatgga actttgttag atgaaaagag
    27001 cgaaataaat ccagagtttt ataaagtatt taaaaaatta agagagagag ggataatgtt
    27061 ttctgctgct agtggcaggc agtatcaaaa tcttataaaa aaatttgaag atataaaaga
    27121 tgatatgatg tttatatcag aaaatggaac actagtagta tataaaggaa aagaaatatt
    27181 atcaaatcct ttaaataaag aattagtaaa tgaaatcata gagacaacaa gaagtataaa
    27241 agggaaaaaa attgtaatga gtgggaaaaa atacgcttat atagaaagca aagatgaagc
    27301 gtttatacaa gaggttagta cttattatgc aaagtttaaa gttgtagaag atttgacgaa
    27361 agtagaatga gacatattga agatagctgt atttgacttc aaaggtgcag aacataataa
    27421 taatatctac tttgaaaagt tttctgatag agctcaagta tgtatttctg gagtggagtg
    27481 gcttgattta actgcaaaag gtgcaaataa agggagtgcc ataaaaaaag tacaaaaaat
    27541 gttagatata aaatatgaag aaactatggt atttggtgac cagttaaatg atgtggaaat
    27601 gatgaaaagt gcttatcata gttatgccat ggaaaatgca aatgaacatt tgaaacaaat
    27661 tgctagattt agagcaaaaa gaaatactga aaatggcgtt gtagataaaa taaaagaggt
    27721 cataaaaatt ggatagtcat atgtatagat gaaaaagatt attttgaaga aatcaaaaaa
    27781 tatcaatgta attttgataa ataagtttat ttatgcgaaa attacattga tatttaataa
    27841 acatcaataa tagatattta tttttttatg atatccagta ttttgttttc tatttgagtt
    27901 gcattttcag cagaaattaa ataaacatat ctacctgcct tctcaattat agtattattt
    27961 gctatttcag aattgatttt ttctccatgt ccttctccaa aaggtgcttg tatcatatct
    28021 tttttataat tttctaaagt tttatagatt ttatctacct cgtcagtttt tataacgata
    28081 atatcttcta gagataattt ttcagaagaa ttaattacaa aaccttgaat tatgtcttcc
    28141 tttacatcat taaaatagtt aaagtccttt gcatcaaaga tttttgattc ctttatgagc
    28201 aaatcagaag attttaatgc ttttttaatt tttgatgtag aaacgttttt gtaatcacca
    28261 cttgaagaac aactaaccat tcctaatgat aagattatcg aaaaaaacaa tattaaaatt
    28321 tttttcatga taaacctcca agttttttaa actctgtctt aaattagttt actacataat
    28381 tatataaaaa taaatgtatt taatgttaca gttttgatta ataatgtaat attttgataa
    28441 taatacctat tgagaggtga ttagaaatga gaataccaaa acatgtagga attatacctg
    28501 atggaaatag aaggtgggca agtcaaaatg gtatgacaaa agataaagga tatgaaagtg
    28561 gaatagaccc tggactagag tcttatagat tatgtagaga tttaggctgt gaagaggtaa
    28621 ctttttatgg atttacaact gataatacaa gacgtccatc agtgcagaca aaagctttta
    28681 caaaagcatg cattgatgct gcaaatatat taatagatga aggtgcagct ttactagttg
    28741 taggaaatac aaaatctcca atgtttccag aagaattatt accatatact aatagaatta
    28801 aagccaaaga aggtcaaata aaagcaaatc ttctagtaaa ttatgggtgg tattgggact
    28861 taaataatat atcaggagta gaaaatgtaa ataaaaataa tatacaggat tacatacagt
    28921 catttgatgt atccagacta gatttgataa tcagatgggg tggaagaagg agattaagtg
    28981 gtttccttcc tgtacaatca atttatgcag atttttatgt tatagataat tattggccag
    29041 attttaagag tgacgatata aaggacgcat ttagttggta tgatacacaa gatgtcactt
    29101 taggtggata attaaaaata ttctgattta aggttgtaga gtgttttaaa ttattgaaaa
    29161 aaaaacttct tgatggtaaa ataacactaa taatatgtaa aagtgtaaaa atttatacca
    29221 agaagggtga agaaatgtca aaaggcaata ataacaataa ttcaagaaac aaatctaaaa
    29281 agaccagtca tctgaataga aaaaagcgaa aattaaataa gaaaaagtta gcagtgttga
    29341 tttgctttac agttttattt ttatttattg catttaaggc tactcaaggt gtagtagctt
    29401 tggtaaaaag tatggataaa agtaataaaa ctagtcaaca acaaaatgtt aactcagaac
    29461 aatttgactt tggtaatgaa gaggaaaata aaaagaaaaa atatactgta tttatagacc
    29521 caggtcatgg aggcaatgat aaaggaactg aaagcaagac cagtaataga tatgaaaaag
    29581 acctaaatct acaaattgca aaaaaattag caaataaatt atctaaacaa aaggatattc
    29641 aagtagtagt atccagaact gatgatacat atatatcatt aaaggatagg gctatcttag
    29701 caaataatag ttctgctgat gtgcttgttt ctattcattt aaatgcagag aaaaatggta
    29761 atacggcaac aggtatagaa acttggtata ggaataaagc tacagatggt tcaaaagaac
    29821 ttgcacaggc agtacaaagt actatagtat cctatgttaa agttagagat aggggaatag
    29881 ttgaaaataa ctttgaagta cttagagaat caaatatgcc tgctatattg atagagtgtg
    29941 gttttttaac aacaccttca gaggaacaaa aaataataaa tgaaaaatat caagaccaac
    30001 ttgcagaagg aattgttcaa ggtgtacttt catacctaga ttctaaaggg aataagtaag
    30061 ttaactttac tttaatgtga ggataaattt aaaagtttaa ctgtgtaatt gtattaactt
    30121 tatttcgtaa tgttggaaac attgtttgaa tttagaaact atgaaattag aaattatttt
    30181 atctatatct aatttcatag ttttttttgc aaaaaattta tagttataaa atttaaaaaa
    30241 ataaatttag tataatgtaa tgtagttatt tatatgtgat tttaacttgc attgatttgc
    30301 tatcaattgt gctttataat taatatatat aaagtttaac tgtaagataa tttaaaaaat
    30361 aggggaattt atatgaataa aaatagtatg acaaaaattt acagtttaaa taaaataatt
    30421 tttattataa ttggaatttt ag
//
LOCUS       DAEPXF010000028        29520 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain HB27
            SAMN13376389-rid12479423.denovo.030, whole genome shotgun sequence.
ACCESSION   DAEPXF010000028 DAEPXF010000000
VERSION     DAEPXF010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13376389
            Sequence Read Archive: SRR10518432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 29520)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29520)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001069624.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001069624.1
            Genome Coverage       :: 315x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/15/2021 01:54:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,165
            CDSs (total)                      :: 4,076
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 89
            rRNAs                             :: 10, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 10 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 19 of 69
            Pseudo Genes (incomplete)         :: 44 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 10 of 69
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29520
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13376389-rid12479423.denovo.030"
                     /strain="HB27"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="China: Hebei"
                     /collection_date="2017"
     gene            complement(<1..1232)
                     /locus_tag="KRL67_001152"
     CDS             complement(<1..1232)
                     /locus_tag="KRL67_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HBH1522050.1"
                     /translation="MKRNLSLLLICLLIFTSFLGRSNISFADNEPAIVAKHAVLMDYE
                     TGKILYNKDGNSKLYPASTTKVWTACLVLKEVKDLNQVIEIKDLPQIDGSSMYLKEGE
                     SFTVKQLLDALLVHSANDAAFVLARYVGGGNVQKFIDLMNSEAKKIGATNTHFNNPHG
                     LPDPNHYTTAHDMALIAREAMNNDTFRQIVKTKSLKFEATKAYPYERYFVNTNKFLTS
                     HDKITYKGQPINIKYDIVDGIKTGYTDAAGKCLLSSAVKDGRRVIVAVFNSTNADLYL
                     DSRILIDYGFDNFKCATIVDKEKYTDTKKVLFTKQHELIYEPKNSYKIFLEKNESKGN
                     YDTKTELNKIDLPIKKGAKVGTLNVYNNGKLENSIDLIAKNNLDSSLPFLTENNVLMT
                     FVKIIAGILILLVLFIITS"
     gene            1312..2016
                     /locus_tag="KRL67_001153"
     CDS             1312..2016
                     /locus_tag="KRL67_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HBH1522051.1"
                     /translation="MKKKVKFISFFVLFILIISSIIVYKSRIFKDDIYVVSKKNLPNE
                     KTHVVETEIKGEDNNHNKATKDIDKNFQNNKITIYISGAVNRPGIVTIESYKRLYDAV
                     ELLGGTTKEADLNGVNLSVRLEDEQHYIIPKIGEATSVTSNDDSNKPNQKNESRGEPK
                     NKSNKNISNESKVNINVATIEELDSLPGVGEATANKILQHREENGQFSSIEEIKNVNG
                     IGDKKYENIKDLICID"
     gene            2108..3154
                     /gene="selD"
                     /locus_tag="KRL67_001154"
     CDS             2108..3154
                     /gene="selD"
                     /locus_tag="KRL67_001154"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015617929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:2156..2158,aa:Sec)
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HBH1522052.1"
                     /translation="MKKEQKKLTEMTTAGGUAAKIGPEVLASVLSQLPKNDNIENLLV
                     GLDTADDAAVYKLNDDMALIQTLDFFTPMVDDPYVFGQIAASNSLSDVYAMGGKPLVA
                     MNIVCFPSCHDMDVLAEILKGGFDKVKESGALLVGGHTVDDKEPKYGLSVSGIVNPNK
                     VLSNATAKPGDKLIITKPIGVGVLNTAMKEGMVEQHIADKVIEIMIHLNKYAAMSFDK
                     FDVNSVTDITGFGLLGHTLEMAKASEVSIEIESKNVPIIEGAIEMAQMGIIPAGMYKN
                     MHYVSKDVEVVGNVEVAVQDILYDPQTSGGLLISVKEELAEELVKDMKLNGAIEAKII
                     GSVVPKGEKYITVL"
     gene            3172..4584
                     /locus_tag="KRL67_001155"
     CDS             3172..4584
                     /locus_tag="KRL67_001155"
                     /EC_number="2.9.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-seryl-tRNA(Sec) selenium transferase"
                     /protein_id="HBH1522053.1"
                     /translation="MIILSKRELFAMLPSVDEVLSDNRIVEIINEYPRSLVLESVREV
                     IDLKRQFILRLKEDASNSVTIEFGEIIESAIERVKLNYSLSLKKVINATGTVIHTNLG
                     RSLLSEDIKDELWCSASRYSNLEYDLDNGERGSRYSHLTSTIKRLTGAEDVLVVNNNA
                     AAVLLVLSTMAKGGEAIVSRGELVEVGGSFRIPSIMALSGAELVEVGSTNKTHLKDYK
                     DAITEDTNVLMKVHTSNYRIMGFTESVSIEELVNLGKKYKLPVIEDLGSGVFIDLSKY
                     GLSYEPTVLDSIRQGADVVTFSGDKMLGGPQAGIIVGKKEYIEKMKKNQLTRALRVDK
                     LTICALEATLRMYLDETKAIENIPTLKMLTYKIEELEVKANKLFEKITALNLNASINI
                     EDGFSQVGGGSMPLETISTKVIAITPEHMNVSSLEKKLRLSEAHIIARVYDNKYVLDV
                     RTIFDDEFDVIVEELRKAFN"
     gene            4698..6581
                     /gene="selB"
                     /locus_tag="KRL67_001156"
     CDS             4698..6581
                     /gene="selB"
                     /locus_tag="KRL67_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenocysteine-specific translation elongation
                     factor"
                     /protein_id="HBH1522054.1"
                     /translation="MKNVVIGTAGHIDHGKTTLIKALTGRETDTLDEEKKRGISINLG
                     FTYFDLPNNRRAGIVDVPGHEKFIKNMLAGASGIDIVLLVVAADEGVMPQTVEHLDIL
                     SFLNIKNGIVVLTKSDTVDDEFRELVKEDICEKIKGTFLEDAEIIEVDSISKRGIDTL
                     IEKIDKMSDDIEDKNENSPARLNIDRVFSIKGFGTVITGTLIEGKISIEDDLVIYPQV
                     LKTKIRSIQVHGENKDTAYAGQRTAINISNVKVDDIKRGDVLAAPNSLEESMMLDVKL
                     SLVNHLDKGLKHWDRLRLYHGTREILCRAVPLDKELIESGESGYVQLRLEESIVSKKG
                     DTFVVRSYSPMETIGGGVIIDPSPKKHKKFDEKVIEALKIKEKGELKDIIEEYLKRNL
                     KNYPNIKEIMSYSGAHEEDVKRALETLISEDRVFIIGNMYMHINQYNKLKENTIKLLS
                     EYHKKYRLRKGILKEEVRSKIESNFKTREMDILLEKLSTENAIKIENNIVSLLDFEVI
                     LNEKQKEIAKKIEKRLKSCGVSSILTIDEVSEGNHNYAEVLESMIGNKVEKLDDLYIM
                     DKDIYENAKNILINYIKENKEITLGEYRDLIDSSRKNCMIILENFDRNKITKRVENKR
                     ILF"
     gene            6686..9424
                     /locus_tag="KRL67_001157"
     CDS             6686..9424
                     /locus_tag="KRL67_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation-associated two-component system
                     sensor histidine kinase"
                     /protein_id="HBH1522055.1"
                     /translation="MKNIIPDVKPDIKEREDMSLLFIIMGSVLLLFIIKDIEKYETLL
                     HAIYTIFLIATGIIIFNTTKFKVDSFSTFLGLIFIATGVLECIYLFNSLGIKTKSIME
                     INITISAITDLFPILGVYLSFKFVKDNKQIYSSVILFIATATLTITSLFIISGICDYV
                     GNGKSQYALGVIISIFIIIISIISGIELNSSSNKGKWEYNEKKIFNRIIVIIILSRVP
                     NLLHIVIDNRNVESILSQIIINVALYYLYNYIVSKNIKKTALELHDTNEELTKKTESL
                     KEKNKKLIYETEKIEELKGILMKRESRLQSTLDVAVNSIVVFSKDGEITYANKSFRNT
                     FGDYKEQDKLTDKIKNFNDLIESIHYVFVHEKNVEKLISTSDNKVYQAIFAPLIIASQ
                     TEGVLCVLEDKTKKKEYERKLIEANKRSRDFLESIGDGIVVLEGNNKIYVNNACREIF
                     KNNLERIDFSLVCKSEESIEKRYVIDGEVKYVEMSFSQYTNAGTNKTIIVIRDTTSRK
                     ISQIKLEESQSSYSRFIDILPDGICLLKEDLSINYANKSFLDMLAFTNIDDIKDSNIK
                     LLMNANTEEKMKFTDNMAKVLNENRSILLLEYELINSYNDIVEVELSALPFAIYNTRY
                     IMLIIKDLVHKKYSEQAEKELLERFKTDKIKTEFFANMSHELKTPLNVISSSNQLVDS
                     FYRSEKIKDYNNNIKSHVDLVRQSSYRLQRLINNIIDLTKMESGFYTLKLAKYNIVSV
                     IEDLFMNIEEYALRKDIKILFDTDLEEINVYIDKVEIERIMLNLLSNCIKFTDNGGWI
                     YVSIHYKIDKVIISVKDTGVGIPQDKLELIFEEFSQVDKTLSRNTEGSGIGLAIVKNL
                     VSLHGGDIEVVSEVNKGTEFLISLPMKGFSSEHYTEDKRIYNIQEKIKIEFSDIYY"
     gene            complement(9543..10487)
                     /gene="manA"
                     /locus_tag="KRL67_001158"
     CDS             complement(9543..10487)
                     /gene="manA"
                     /locus_tag="KRL67_001158"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase, class I"
                     /protein_id="HBH1522056.1"
                     /translation="MEPLFLKPIFMDRIWGGTALKDKFNYEIDSPTTGECWAISSHKN
                     GDCLIENGKYKGKKLSELWDKNRELFGNTPGDKFPLLTKILDANDNLSVQVHPNDEYA
                     KKNENGELGKTECWYVIDCSDDAEIIIGHNAKSHKEFVNMVNNNEWDKLLRKVNIKKG
                     DFFYVPSGTIHAICKGTLILETQQNSDTTYRVYDYDRTDNSGNKRELHIQKSIDVTNV
                     PHINFDTDYKIVSTSDFKCTTFVSNEFFSVYKLDVFGKCNFTHNTPFSLYSVLDGNGK
                     LIHNSVEYNLKKGMHFILPNDFGDFSFEGNLEIICSHI"
     gene            10660..12021
                     /locus_tag="KRL67_001159"
     CDS             10660..12021
                     /locus_tag="KRL67_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HBH1522057.1"
                     /translation="MELFKTESKKYEKEFLKLLEEWVSIPSFYDRKTVSKDMPFGKGV
                     YDALNWFENLGRENNFKVKNIDNHAVQIEYGNGKEYVDIFGHCDVVNPGEGWDSEPFK
                     LNIIGDKLVARGVSDNKGPMIVNFLALKMIKDLDINLKRKVRLIAGGNEESGFKCIKH
                     YYSKEPYGVCGFTPDAKFPVLNGEKGGAIIKLISNIDDKSLYISGGIEFNTIPDKVYI
                     KNIEKLGKDNICFDINNISINYDNGNYIVHGKGGHSSKPEKSINPILATIKLLSENID
                     EKWTKDLYKLINQDNINGNLFGLNIEGKCGILSMVPTIINIVDGKLEVVLSVRYPEIL
                     TIEDIMKKFNLYMEQNNINKFELIGENLKQANYIDRNSKLVRSLHDIYIKYSGDLKND
                     VRVTSAGSYASEMNNSVIFGCEFPDGSFGNVHSANEFASLDRLIDAIGIYAEAIITLC
                     NKI"
     gene            12109..13998
                     /locus_tag="KRL67_001160"
     CDS             12109..13998
                     /locus_tag="KRL67_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HBH1522058.1"
                     /translation="MITKKHAVIVKSLSNKDGYMTSNELAIKLDVSTKTIKRYIADLN
                     SVLSKYDLVIASSRGIGYKLSGSKNNIARAVKEANKYIDGFLDDSEESRMSNIICMLI
                     NRNYMSIEAMAEELNLSIAAINKLSSKLKRKLEKYDLVIKSKPYYGSYIYGEEINIRQ
                     LITDYAIKLDEKSKVKVFLDDISENDIYCIESILEKHLRKKDTIISDKDFNLLLSKII
                     VSVFRSKRGHSNNINLMDTSYRFHNYYFIESLMKEISDKIGFKLIEDEVIYISNYSGV
                     IAYKGTQGRKNTSEIEERISSVISSALQDIFLISGSDYTKDEEFMVAISDHIKRFLNR
                     AKANIKSNNPLLHQIKEKFPIAFNLAVFLSNKLEAEFNLKLDEDELGYIAIHFAASNE
                     RMKKNTSKKICIVCHYGIGTGQLISEKLKQNISDLSVVGVYPVRYLDMAISQDVDLIV
                     STVELKGYEKPVLYIENIFDDSLIENVNKAFYEKEERRKIISNMFDEKAFFSIKANTK
                     EEVILSLSNKLKERDFIEESSIKSIIDRENISSTEIGNLVAIPHTIVKGDKKSIIGVG
                     ILENPIIWDKQEVQLVFMVFFNTKEKHNFSIFKYLYNFIKDEGGVRGIIKICDFNKLM
                     ALIGN"
     gene            14013..14468
                     /locus_tag="KRL67_001161"
     CDS             14013..14468
                     /locus_tag="KRL67_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBH1522059.1"
                     /translation="MGTNIFNKEYVFLNVDAKSKVEVLKFISKKAKDLNLAEDESLVY
                     EGLMAREEQFTTNLGESIAIPHTKNDAIENPAVVVLKFNEDVVWNEGEDKVKLAISLL
                     MPGKSKENIHLKLLSSLSRKLINKEFKDSLLKSDNIEEISNLINEALGL"
     gene            14527..14844
                     /locus_tag="KRL67_001162"
     CDS             14527..14844
                     /locus_tag="KRL67_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HBH1522060.1"
                     /translation="MNKKLVALCACPMGLAHTFMAAEAIEQAAKALGYEAKVETQGAD
                     GVQNELTRDDILGATMIIHAVAITPEGMERFDGCEVYEVELQEAIKNAEGVIKEIEED
                     LGI"
     gene            14869..16131
                     /locus_tag="KRL67_001163"
     CDS             14869..16131
                     /locus_tag="KRL67_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HBH1522061.1"
                     /translation="MAIKKKVINSSGASTTGGNNVGKSTPSSPTQSKGNGKWKEISKH
                     IMTGISYMIPVLVMGGLIGALSQLIPYAILGLDPSVGIVDAMNSGEFTGFKLSLLNIA
                     QLMSNFGFTLFGFAIPLFAAFCANSIGGKTALIAGFIGGYIANKPVGVPQFVDGQWTE
                     VVPVASGFLGAILIAFIIGYFVKWLNKSIKVSHNWLAFKTTFLIPLIASLACMVLMIF
                     IITPFGGLINESMKNFLTAAGAAGEYVYATALAAATAFDLGGPINKAAGFVALGLTTE
                     NVLPITARTIAIVVPPIGLGLTTLLDKRIVGRRVYDRQFYQAGKTSIFLSFMGISEGA
                     IPFALERPGFVIPLNIVGSVIGAITGIILGAIQWFPESAIWAWPLVDNLFGYIIGIAV
                     GAIFIAVGNIFYRNKLIKDGKLVVDYID"
     gene            16236..17300
                     /locus_tag="KRL67_001164"
     CDS             16236..17300
                     /locus_tag="KRL67_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HBH1522062.1"
                     /translation="MNKIEKVREYFIEYDIDGFLINSPTNKFFVGNLFSSSGYVFITK
                     EAQYIIVDFRYFEEIKRKSSLFNVVLMDKTRTHFDIINDICREQNIKEIGFEGNEVSF
                     DLYRSMSNKLSATLKSVDLSTLREIKNEDEIKYIKKACEIVDATFYHIIDFIKVGMTE
                     KQVENEIVRVIKDLGGQKESFDTIVASGLRGALPHGKASEKVIEYGDFVTFDFGAKYN
                     NYCSDITRTICMSTINKELEEIYNIVRKANEECIRVLRPGMTTGEIDKVARDIIGSYG
                     YADNFGHNLGHGVGIMVHEYPALAPESNEVLKEGMIVTIEPGIYVPSLGGVRIEDDVL
                     ITQDGCMRLTTSTKDLIVVS"
     gene            17354..18532
                     /locus_tag="KRL67_001165"
     CDS             17354..18532
                     /locus_tag="KRL67_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PatB family C-S lyase"
                     /protein_id="HBH1522063.1"
                     /translation="MYNFDDKPDRVSEKCRKWDLNIIRDKFGDIREDFVPMWIADMDF
                     KIPTEIENKFIEAVRRGVFGYTYCYDEFYDSVIGWQKNMHEVHVEKEWITLTYGTVST
                     LHYVVQAFCKKGDSIILNTPVYDPFESSAKKQGVNVICNTLDVVNDRYYINFDKLEVQ
                     IKENKPKLMMFCTPHNPSGRIWTIEEMTRVATICKENNVILVADEVHAEHIHYGKFNS
                     ILKIEKELLENVILLTSPNKGFNLGGLKTSYSIVINKDIRDKFRNKLKQNSITSPNVF
                     GIIGLITAYNECHEWLSGVNEYIKSNYELLETWVNKYNKIKLMKMESSYLAWMNISGL
                     GISASEFTDRLAIDTGVLLEDGSHFVKDGEKYVRINLGTQKENVIEALNRMDLFLKSL
                     "
     gene            complement(18713..19768)
                     /locus_tag="KRL67_001166"
     CDS             complement(18713..19768)
                     /locus_tag="KRL67_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HBH1522064.1"
                     /translation="MNKKKITFIFVILILALILFSFFRSSSQNTKYSKTSYYLGTVNE
                     VTVFNVRESKSDKILNECDSILRYIDNKMSTHIPGSDVSKINDNAGKKFVRVSDDTFF
                     VVKEAIQYSKLSDGYFDITIGPLSNLWAIGTDKAKVPSDSEIQKLLPLIDYKNIILDE
                     KNKSIKLTKKNMKIDLGAIAKGYAADKIVAYLKSENVKKSLVNLGGNIFTLEDGKNDK
                     PFKIGIQDPTNKNGESIGNIETTNKSVVTSGIYERFIEKDGKMYHHMLNPFTGYPFEN
                     NLSSVTIISDKSINCDALSTSTFGLGLEKGMDLINKIDNVDAIFITKDKKVYLTKGIK
                     DNFKLTDKSFSIEKLNK"
     gene            19835..20206
                     /locus_tag="KRL67_001167"
     CDS             19835..20206
                     /locus_tag="KRL67_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HBH1522065.1"
                     /translation="MKKKDILLILGILVVITACYGIINVINSKNAGNIEIYVDNKLYK
                     TVSINAKEEFKIENRGGYNIVKIHDKGVEIVDASCPDKVCVHTGFINKPSQSIVCIPN
                     RVSIKINTNEKNDNQEDIISK"
     gene            20243..20917
                     /locus_tag="KRL67_001168"
     CDS             20243..20917
                     /locus_tag="KRL67_001168"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HBH1522066.1"
                     /translation="MVNLGNDWDELLKEEFEKDYYLNLRKFLIEEYKTRQIFPNMHNI
                     YEALKHTSYKDTKVLILGQDPYHGDNQAHGLAFSVQPQVKTPPSLLNMYKELKDDLGC
                     FIPNNGYLMPWADQGVLLLNTALTVRAHEANSHKNKGWEIFTDRVISILSEREDPVIF
                     VLWGSNARKKVELIDTSKHYILEAPHPSPLSASKGFFGCKHFSKINEILKKLGKEPIN
                     WQIENI"
     gene            21165..22004
                     /locus_tag="KRL67_001169"
     CDS             21165..22004
                     /locus_tag="KRL67_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="G5 domain-containing protein"
                     /protein_id="HBH1522067.1"
                     /translation="MNFKKLSNAKVSVMAVVLSVGILSGYSALNKQVTLVVRGEERTI
                     STFSSNVGDLLESQNISYDANDIVTLDENTKLSNGDKIEVIDVKEKTVVETKDIPFEV
                     TVVEDGSLLKGDSKVKEEGKSGKSELIYKETYHNGKKVEKEFVKKVVKAEPINKVVSK
                     GTKVEMQVASSRGESSRRMASSSSSSSSSNNGKNMRVVATAYTGHSITATGTKPKWGT
                     IAVDPKVIPYGTKVYIPQFDMVFTAEDCGGAIKGNKIDIYMNDSKTVYNWGRKTIDIR
                     ILK"
     gene            22269..22365
                     /locus_tag="KRL67_001170"
     tRNA            22269..22365
                     /locus_tag="KRL67_001170"
                     /product="tRNA-Sec"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:22303..22305,aa:Sec,seq:tca)
     gene            22516..23223
                     /locus_tag="KRL67_001171"
     CDS             22516..23223
                     /locus_tag="KRL67_001171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA threonylcarbamoyladenosine dehydratase"
                     /protein_id="HBH1522068.1"
                     /translation="MENNFTTRTSFLVGDDGIEKLNNSNIIVFGVGGVGSFTVEALAR
                     AGVGNITIVDFDDVDITNINRQIPALHSTVGRYKVDVMEERILDINPNINIKKIRSLY
                     NKDTSDEILTERYDYVVDAIDMVSSKIHLIETCEKKGLKIISSMGMGNKLDPTKIVVT
                     DIHKTSTCPLAKVMRKELRDRGIKKLKVVYSTEQPIELKKKVMNGRKVTPGSVSFVPS
                     VGGLIIASVIVNELLGQ"
     gene            complement(23365..24201)
                     /locus_tag="KRL67_001172"
     CDS             complement(23365..24201)
                     /locus_tag="KRL67_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HBH1522069.1"
                     /translation="MRNKIFLSILIIISLLIGCDKKSLLSIHMIDVGQGDSILVQTPT
                     NKNILIDGGDEDSENIIISYLRQKRIKTIDIIIATHPDSDHIGSLDNVIKKFNVNSIY
                     MPEQSTDSEAYQNLINSCTDKNLSIQHLYKNDVLNIDNNINIYVLSPSYIQEESNLNS
                     IVFKLTFNDNSFLFMGDAEEENEKEILHSFKLNNINFIKIGHHGSNSSSSLEFIKKIS
                     PDIAAISCGYKNQYGHPHRKVIDNLKQNHVSIYRTDRIGDIVFYSDGEIIFTKYNYEI
                     DT"
     gene            24389..25294
                     /locus_tag="KRL67_001173"
     CDS             24389..25294
                     /locus_tag="KRL67_001173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2156 domain-containing protein"
                     /protein_id="HBH1522070.1"
                     /translation="MFFKDIELNSKKELDPYFDLVDYEACEYCFSTLYMWQHVYKTGY
                     YIGEDFAVLVGEYEGDSFSILPLAKKDKLPEVVDFVLEYFSKNNKKIYLRGITTEVVE
                     FLKEKYPGRFEYIEERDLFDYIYDAESLRTLAGKKNQKKRNHINYFLKEYAGRHEAKL
                     LDKENFDECLVLMKEWESNKEENNEFDESMDDELIGIKKIFNHYDILKDKVKVFGVYV
                     DGKLEAFSIGELLNPNMALIHIEKANPDIRGLYPFINQQFLVSEFKDVEFVNREEDLG
                     IEGLRKAKLSYHPCRFVEKYSVREA"
     gene            25303..26487
                     /locus_tag="KRL67_001174"
     CDS             25303..26487
                     /locus_tag="KRL67_001174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBH1522071.1"
                     /translation="MEIRYAKEEEIESIKEIWSYCFNDTESFMKYYFNDKYKSENTVV
                     ALDEGKIISSLQLNQYKLLLNSKVYNTSYVVGVSTLPEGRGAGYMNKVMKFTLNELYK
                     KGQLVSILMPIDYRLYRRFGYEHCYDQIEYTINTDDLKNFKSSGKMIKSDLSQIDDLI
                     RIDRTFLNEVNGNVLKDEHYYENLFKEIQSEDGFLYIHEGNEKDGYIVYFLQEGKMFV
                     RELFYKNIDALKSMLKFIYNHNTQCKIVTISTPTIDKIRFILDNPKDSDIKIKPFMMG
                     RIINVKKFIEDIDIEKDINSSFNLLIEDKFIDENNGLFKISIQNKKVSVEQLDKKGAE
                     KPQEDFDIKLDINTLTQLSFSYIDVNEAIFLNDIKDVSEETLETLNCIFSKKNTYINE
                     YI"
     gene            26602..27903
                     /locus_tag="KRL67_001175"
     CDS             26602..27903
                     /locus_tag="KRL67_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HBH1522072.1"
                     /translation="MGVIIIQIRRPLLIILMFVVAISFIITNNTKNDEELNDKIITIQ
                     GVVKGKIEKKRYNQYKVGVFLINDYTKNKNLKIGQKVNITGKFKSLDKMKYDDFDYGR
                     YIKSTGYKGIVYINNYKIIDKNKLYSLIGKIKFYISKTYRYLYKKNSDFINSILLAEV
                     ENLTEEQKEIFTRTGTSHVISISGLHTGILCVIISFLLRGINKLYKLLILSIFITLYC
                     IMVGASPSIIRSIAFVMIFYLSIFIDRKKDGISALSLIGIILIMNNPYVIYNVSFQLS
                     FLATLSILIFYNKINSIIKLSMVSLTISSNILTLPIIYYTFKGIPLLSIIGNLIIVPF
                     VGVIMYLSIASLIVFKVSVVIAKIISFFNSILIESIFFLLEKISNLSFAYTNIESPKF
                     YIVVIYYIGVFFYIFYIEGKEIKEQENELQGYYKECKREKL"
     gene            27857..28900
                     /gene="holA"
                     /locus_tag="KRL67_001176"
     CDS             27857..28900
                     /gene="holA"
                     /locus_tag="KRL67_001176"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HBH1522073.1"
                     /translation="MNYKDIIRNVKEKNFEKMYLFYGREYYLIENAIKAFKDSLNEGM
                     LDFNLDIIDGKEIVLNQLISSIETLPFMDDRKIVIIKDFELLKGKKKNFTDSDEKYLI
                     EHLDNIPDTTTIVFVVYGDVDKRKSLVKKIGNNGIVFDCDKLSDMDLFKWIKKSFALN
                     DVIIDNSQIMYFIEQEGYRDKSSEKTLSDLENEINKISSFVGKGNNVTNDVIDKLSQK
                     KVENDIFKLIDYIGEQNASNAMKILNDMIQEGESVLGIFSMIARQFKIIMQVRQLQLD
                     GYSTKLIADKLKMHQFVVGKALKQTKNFSDDIIVEILNYILESDYKIKTGLIRDTLAV
                     EMLVSRYCKREAI"
     gene            complement(29087..29353)
                     /gene="rpsT"
                     /locus_tag="KRL67_001177"
     CDS             complement(29087..29353)
                     /gene="rpsT"
                     /locus_tag="KRL67_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HBH1522074.1"
                     /translation="MANIKSAKKRISVIEKKTALNRVRKSQIKTAIRRFEDAVAAGNR
                     EDAVAKFQYAQKRIYQVASKGTIHKNAAARKVAKLAQKLNGMNA"
BASE COUNT        11760 a         3066 c         4953 g         9741 t
ORIGIN      
        1 gaagttatta taaataaaac taaaagtatt agaatacctg caataatctt aacaaaagtc
       61 attaaaacat tattttcagt caaaaatgga agactgctat caagattatt ttttgcaatt
      121 aagtctatac tgttttctag tttaccatta ttatatacat ttaaagtacc aaccttagcc
      181 ccttttttta taggcaaatc aattttattt aactctgttt ttgtatcata attgccttta
      241 gattcatttt tttctaagaa tatcttataa ctatttttgg gttcgtaaat caattcatgt
      301 tgttttgtaa acaatacttt tttagtatct gtgtattttt ctttatcaac aatagtagca
      361 cacttaaagt tatcaaatcc ataatcaatt agaatacgtg agtctaaata taaatcagca
      421 ttagttgaat taaatacagc aacgataact cttctgccat cttttacagc actcgataaa
      481 agacatttac cagcagcatc agtatatcca gtttttatac catcaactat atcatatttt
      541 atatttatag gctgaccttt gtatgtaatc ttatcgtgtg atgtcaaaaa cttattagtg
      601 tttacaaagt atctctcata aggatatgct tttgtagctt caaatttaag tgacttagtt
      661 tttacaattt gtctaaaagt atcattattc atagcttctc tagctatgag agccatatca
      721 tgagctgttg tgtagtgatt tgggtctggt aatccgtgag gattattaaa atgagtatta
      781 gtagccccaa ttttttttgc ctcagagttc ataaggtcaa taaatttttg tacatttcca
      841 ccaccaacat atctagctaa tacaaaagca gcatcattag cagaatgaac caaaagagca
      901 tctagtaact gctttacagt aaaagattcc ccctctttta gatacatact agaaccatct
      961 atttgaggta aatctttaat ttctattact tgatttaaat ctttaacctc ctttagaact
     1021 aaacaagctg tccatacttt agttgttgaa gctggataaa gcttagaatt tccatcctta
     1081 ttatatagta ttttgccagt ttcataatcc attaaaactg catgtttagc aactatagct
     1141 ggttcattat ctgcaaaaga gatattagat cttcctaaaa atgaagtaaa tatcaataaa
     1201 cagattaata aaagtgataa atttcttttc attgtgagcc tccaaatttt aaattcatct
     1261 tttagtatag catatatttg tcaataatta aaagaaagga gtgattaata tatgaaaaaa
     1321 aaggtaaaat ttatttcgtt ttttgtttta tttattttaa taatatcatc aataatcgtt
     1381 tataaatcgc gtattttcaa agatgatatt tatgttgtaa gtaaaaaaaa tttacctaat
     1441 gaaaaaacac atgttgtaga gactgaaatt aaaggtgaag ataataatca caataaagca
     1501 actaaagaca tagataaaaa ttttcaaaat aataaaataa ctatatacat aagtggagca
     1561 gtaaatagac ctggaattgt aactatagag tcatacaaga gactttatga tgctgtagaa
     1621 ttactaggag gaacgacaaa agaagccgat ttaaatggag tcaatctatc agtaagatta
     1681 gaagatgaac aacattacat aatacctaaa ataggtgaag caacatctgt tacaagtaat
     1741 gatgactcca ataaaccaaa tcagaagaat gagtcaagag gtgagcctaa aaataaatcc
     1801 aataaaaata tatctaacga atcaaaagta aatataaatg tagcgactat agaagagttg
     1861 gattctctac ctggggttgg agaagctaca gccaataaaa ttttacaaca cagagaagaa
     1921 aatggacagt ttagttcaat tgaggaaata aaaaatgtaa atggtattgg agataaaaag
     1981 tatgaaaata taaaagattt aatttgtata gactaaaata taatacaaaa cttacttata
     2041 gatatgttat tataaatatg tatgtatatg tgtgtatatg tggaaattta gtaaaggagg
     2101 cacttttatg aaaaaagaac aaaaaaaact tacagagatg acaacagcag gtggttgagc
     2161 cgctaaaata gggccagagg ttctggcatc agttttatct caattgccta aaaatgataa
     2221 tatagaaaat ttattagtag gactagatac agcagatgat gctgcagttt ataaattaaa
     2281 tgatgatatg gcacttattc aaactttaga cttctttaca ccaatggtag atgacccata
     2341 tgtatttggt caaattgctg catcaaattc actatcagat gtatatgcta tgggaggcaa
     2401 acctttggtt gctatgaata ttgtttgttt tccatcatgc catgatatgg atgtacttgc
     2461 agaaatatta aaaggcgggt ttgataaagt gaaagaaagt ggagcacttt tggttggagg
     2521 gcatactgta gatgacaaag agcctaaata tggtctttca gtttctggga ttgtaaatcc
     2581 aaataaagtt ttatcaaatg ctactgcaaa acctggtgat aagttaatta taacaaaacc
     2641 aattggagtg ggtgtattaa acacagccat gaaggaaggt atggtagaac aacatatagc
     2701 agataaagta atagaaataa tgatacattt aaataaatat gcagcaatga gctttgataa
     2761 atttgatgta aattcggtta cagatataac tggctttggt ctacttggtc atactttaga
     2821 aatggccaaa gcttccgaag taagtattga aattgaatca aaaaatgtac caattattga
     2881 aggtgcaata gaaatggcac aaatgggaat tatacctgct ggtatgtata aaaatatgca
     2941 ttatgtatct aaagatgtag aagttgtagg aaatgtagaa gttgcagttc aagatatttt
     3001 atacgaccca caaacatcag gagggttgtt aatttcggta aaagaagaac ttgcagagga
     3061 acttgtaaaa gatatgaaat taaatggagc aatagaagct aaaattatag gttctgtagt
     3121 tccaaaaggg gaaaaatata ttacagtttt ataaaatatt atagagaaag agtgattatt
     3181 ttgagcaaaa gagaattgtt tgcaatgttg ccttcagttg atgaagtatt aagtgataat
     3241 agaattgtag aaataataaa tgaatatcct agaagtttgg ttttagaatc tgtgagagaa
     3301 gtaatagatt taaaaagaca atttatatta agattaaaag aagatgcatc aaattctgta
     3361 acaatagagt ttggtgaaat aatagaatct gcaatagaga gagttaagtt aaattactca
     3421 ttatctttaa aaaaagttat aaatgcaact ggtacggtta ttcatacaaa tttaggaagg
     3481 tctttactta gtgaagatat aaaagatgaa ttatggtgtt ctgcttcaag atattcaaac
     3541 ttagaatacg acttggataa tggagaaaga ggttcaagat actctcattt aactagtaca
     3601 ataaaaagac ttactggagc agaagatgta ctagttgtaa ataataatgc agcggcagta
     3661 cttcttgtac ttagcacaat ggctaaagga ggagaagcaa tcgtatctcg tggagaatta
     3721 gttgaagtag gtggctcatt taggattcca agtataatgg ctcttagtgg tgcagaatta
     3781 gttgaagtag gttcaactaa taaaactcat ctgaaagatt acaaagatgc cattacagaa
     3841 gatacaaatg tattgatgaa ggttcataca agcaattata gaatcatggg attcacagag
     3901 agtgtttcta tagaagaatt agttaattta ggtaaaaaat ataaactgcc tgtaatagag
     3961 gacttaggaa gtggagtttt tatagattta tcaaaatatg gtttatctta tgaaccaact
     4021 gtattggatt ctattcgaca aggtgcagat gtagtaactt ttagtggaga taagatgctt
     4081 ggaggtccac aagcaggtat tatagttggg aaaaaagagt atatagaaaa aatgaagaaa
     4141 aatcaactta ctagagcact tagagttgat aagcttacaa tatgtgctct tgaagcaaca
     4201 ttgagaatgt atttagatga gacaaaagct atagaaaata tacctacttt aaaaatgttg
     4261 acatataaaa ttgaagaatt agaagtaaaa gcaaataaac tatttgaaaa aataactgct
     4321 ttaaacttaa atgcaagcat aaatatagaa gatggatttt cacaggtggg aggagggtct
     4381 atgcctctcg aaactataag tacaaaggta atagcaataa ctcctgaaca tatgaatgtt
     4441 tcatctttag aaaaaaaact aagacttagt gaagcacata taatagccag agtgtatgat
     4501 aataaatatg tactagatgt aagaactata tttgatgatg aatttgatgt aatagttgag
     4561 gaattgagaa aagcatttaa ttaaataaat ttgggttaac aaatattaaa tttagaagta
     4621 taagtatgaa gttttgatta aaaaatagct acacacactt atgactttag tcataggagg
     4681 tttagagggg gcaaaaaatg aaaaatgtag taattggaac agcaggtcat attgaccacg
     4741 gaaaaactac tcttataaaa gcattgactg gaagagagac tgatacatta gatgaagaaa
     4801 aaaagagagg tatttcaata aacttaggat ttacttactt tgatttacca aacaatagaa
     4861 gagctggaat tgttgatgtc ccaggacacg aaaaatttat aaaaaacatg ttagctggag
     4921 catcaggaat tgatatagtt ttacttgttg tagcagcaga tgaaggggtt atgccacaga
     4981 cagttgagca tttagatatt ctaagtttct taaatatcaa aaatggaata gtggttctaa
     5041 ctaagtctga cactgtagat gatgaattta gagagctagt aaaagaagat atttgtgaaa
     5101 aaataaaagg aacttttctt gaagatgctg aaataattga agttgattct atatctaaaa
     5161 gaggtataga cacacttatt gaaaaaatag ataaaatgag tgatgatata gaagataaaa
     5221 atgaaaactc tcctgcaaga cttaatatag acagagtatt ttcaataaaa gggtttggta
     5281 ctgtaataac aggtacttta atagaaggaa agataagtat agaagatgat ttggttatat
     5341 atccacaggt attaaaaaca aaaataagaa gtatacaagt tcatggagaa aataaagata
     5401 cagcttatgc aggacaaaga acagcaatca atatatcaaa tgtaaaagtg gatgatataa
     5461 aaagagggga tgttttagct gcaccaaact cattagagga atcaatgatg ttggatgtaa
     5521 aattatcatt ggtaaatcat ttagacaaag gattaaaaca ctgggacagg ttgagattgt
     5581 atcatggaac tagagagata ctttgtaggg cagttccact agataaagaa ttaattgaaa
     5641 gtggagaaag tggctatgtt cagttaagac ttgaagaaag catagtatct aagaaaggcg
     5701 atacatttgt agttagaagt tattctccaa tggaaactat aggtggaggt gtaataattg
     5761 acccatctcc taaaaaacat aagaaatttg atgaaaaagt tatagaagca ttaaaaataa
     5821 aagaaaaagg cgaactaaaa gatataatag aagagtactt aaaaaggaac ctaaaaaatt
     5881 atccaaatat aaaagaaata atgagctata gtggtgctca tgaagaagat gtaaaaagag
     5941 cacttgaaac acttatatca gaagatagag tatttattat aggaaatatg tatatgcata
     6001 taaatcaata caataaatta aaagaaaata caataaagct tttaagtgaa taccataaga
     6061 agtataggct tagaaaaggg atattaaaag aagaagtaag gtctaagata gagagtaatt
     6121 ttaagactag ggagatggat attttattag aaaaattatc tacagaaaat gctataaaaa
     6181 tagaaaataa tatagtatct ctattagatt ttgaggttat tttaaatgag aagcaaaaag
     6241 aaatagcaaa aaaaatagaa aaaagattaa aatcatgtgg agtttcttct atactgacaa
     6301 tagatgaagt ttctgaagga aatcataact atgctgaagt attggagtca atgataggta
     6361 ataaagtaga gaaattagat gatttgtata taatggacaa agatatttat gaaaatgcaa
     6421 agaacatttt gataaattat ataaaggaaa ataaagaaat aacattagga gaatacagag
     6481 atttaattga ttctagcaga aagaattgca tgattatatt ggagaacttt gatagaaaca
     6541 aaataactaa aagagtagaa aacaaaagaa ttttgtttta gcagaaaatg tctgtttttg
     6601 ctttaaggta taaagaagga attataatgt cgagtatcgt ataattaatt agtataaaat
     6661 attaattaag aggaagtgta aagtattgaa gaatataata cctgatgtaa aaccagatat
     6721 aaaagaaaga gaagacatga gccttttatt tataataatg ggctcagtac ttttattatt
     6781 tattataaaa gatatagaga aatatgaaac tttattacat gctatatata ctatattttt
     6841 aatagctacg ggaataatta tatttaatac tacaaaattt aaagtagatt cattttctac
     6901 atttttagga ttaattttta tagctactgg agtattggag tgtatatatt tatttaattc
     6961 cttggggatt aaaactaagt ctataatgga gattaacatc acaatatcag caataacaga
     7021 tttatttcct atactgggtg tatacttaag ttttaaattt gttaaggata acaaacaaat
     7081 ttattcaagt gtaattttat ttatagcaac tgctacatta acaataacat ctttattcat
     7141 aatatctgga atttgtgact atgtgggaaa tggtaaatca caatatgctt taggggttat
     7201 aatatctata ttcataataa ttatttctat tattagtgga attgaattaa attcaagttc
     7261 aaataaaggt aaatgggaat ataatgaaaa aaagattttt aacagaataa tagtaataat
     7321 aatattatca agagttccaa atctattgca tatagttatt gataatagga atgtagagag
     7381 tatattatca cagataataa taaatgtagc cttatattat ttatataatt acatagtatc
     7441 taagaatatt aagaaaacag cactggaatt gcatgataca aatgaagaat taacaaaaaa
     7501 aacggaaagt ttaaaagaaa agaataaaaa attaatctat gagacagaaa agatagagga
     7561 acttaaggga atacttatga aaagagaatc taggctacaa tctacattag atgtagcagt
     7621 aaatagcata gtagtattta gtaaagatgg agaaattaca tatgcaaata agagctttag
     7681 aaatactttt ggagactata aagaacaaga taaattgact gataaaataa aaaacttcaa
     7741 tgatttgata gaaagcattc attatgtgtt tgttcatgag aagaatgtag aaaaacttat
     7801 tagcacaagc gataataaag tttatcaagc tatatttgca cctttaatta ttgcttcaca
     7861 aacagaagga gtattgtgtg tactagaaga taagactaag aaaaaggagt atgaacgcaa
     7921 acttattgaa gcaaataaga gaagtagaga ttttttagaa agtattggag atggcatagt
     7981 agtgcttgaa ggtaataaca aaatatatgt taataatgca tgtagggaaa tttttaaaaa
     8041 taatttagaa agaattgatt tttcattagt atgtaaaagt gaagaaagta tagaaaaaag
     8101 atatgtaata gatggtgaag taaagtatgt agagatgagt ttttctcagt atactaatgc
     8161 tggtactaat aaaactatta ttgtaatacg tgatacaacc tctcgaaaaa tatcgcaaat
     8221 aaaacttgag gaaagtcaat catcatattc aagatttata gatatattac ctgatggcat
     8281 atgcttatta aaagaagatt taagtataaa ctatgcaaat aagtcgtttt tagatatgtt
     8341 agcttttaca aacatagatg atataaaaga ttctaatatt aaactattaa tgaatgcgaa
     8401 tactgaagaa aaaatgaaat ttacggataa tatggctaaa gttttaaatg aaaatagaag
     8461 tatactttta ctggaatatg aattgataaa tagttataat gatatagtag aagtagaatt
     8521 aagtgcatta ccttttgcta tatacaatac tagatatata atgcttatta taaaagattt
     8581 agtacataag aaatattccg agcaagcgga aaaagaactg cttgaaagat ttaaaacaga
     8641 taaaatcaaa acggagtttt ttgcaaatat gtctcatgaa ttaaaaactc cactaaatgt
     8701 tatatctagt tcaaatcaac tagtagattc tttctataga agtgaaaaga taaaagatta
     8761 caataacaat attaaatctc atgtggattt agttagacaa agttcttata ggcttcaaag
     8821 attaataaat aacattatag atttaacaaa gatggagtcc gggttctata cactaaaact
     8881 tgcgaaatac aatattgtat ctgtaataga agacttattt atgaatatag aagagtatgc
     8941 acttagaaaa gacataaaaa ttctttttga tacagacctt gaagaaatta atgtttatat
     9001 agacaaggtt gaaattgaga gaattatgtt gaatctattg tcaaactgta ttaagtttac
     9061 tgataatgga ggctggatat atgtaagtat tcactataaa atagataaag taatcataag
     9121 tgtaaaagac actggagttg gaataccaca agataaatta gaattgattt ttgaagaatt
     9181 ttcacaagtt gataagactt tatccagaaa tactgaagga agtggaatag ggcttgctat
     9241 agttaagaat ctagtaagtc ttcatggtgg agatatagaa gtagtaagtg aagtgaataa
     9301 aggcacagag ttcttaattt cacttccaat gaagggattt agtagtgaac attacacaga
     9361 agataagaga atatataata ttcaagaaaa aataaaaata gaattttctg atatatacta
     9421 ttaaaaatga ttatgagcaa aaataaaata aaaatactat ttgtttaaat atttaagttg
     9481 taagaaatag gctatcataa ggtaaattat tatgatagcc tatttttagt taggttttat
     9541 atctatatgt gagaacatat aatctctaaa ttaccttcaa aactaaaatc tccaaagtcg
     9601 tttggtagta taaaatgcat accctttttt aaattgtatt ctacagaatt gtgtataagt
     9661 tttccatttc catccaacac actgtataaa gaaaaaggtg tattgtgtgt aaaattacat
     9721 ttcccaaaaa catctaattt ataaacacta aaaaattcat ttgacacaaa tgttgtgcat
     9781 ttaaagtctg acgttgatac gattttatag tcagtatcga aatttatatg aggaacattc
     9841 gtaacatcta ttgatttttg aatgtgaagt tcacgcttat tacctgaatt gtcagttcta
     9901 tcataatcat agactctata agtagtatct gaattttgtt gagtttctaa aattaatgtt
     9961 cctttgcata tagcatgtat agtaccactt ggaacataga aaaagtctcc cttttttata
    10021 ttaacctttc taagtagttt gtcccattca ttattattta ccatatttac aaattcttta
    10081 tgagattttg cattatgacc tattataatt tctgcatcat cagaacaatc tattacatac
    10141 caacactctg tcttacctaa ctctccattt tcattttttt tagcatattc atcatttgga
    10201 tgaacttgaa ctgataaatt atcatttgca tccaatattt ttgtaagtaa aggaaattta
    10261 tctccaggtg tgtttccaaa taattctcta tttttatccc acaattcaga tagtttttta
    10321 cctttatatt ttccattttc tattaagcaa tctccatttt tatgagaact tatagcccaa
    10381 cattcaccag ttgttggtga atctatttca taattaaatt tatctttaag tgcagtacca
    10441 ccccatattc tatccataaa aataggtttt aaaaataatg gttccataat cctgttcctc
    10501 ctatgatatt aaatttatcc tacaaataat tatatataaa tagattataa aatcaatagt
    10561 aaaaaagttt tgtcccttga aatgacataa agtataagga gttattaaat aagataaact
    10621 atgatatata atataaagtt ctaaattgga gtgataatta tggaattgtt taagacagaa
    10681 agcaaaaaat atgaaaaaga atttttaaaa ttattggaag agtgggttag tataccttca
    10741 ttctatgata gaaaaactgt atctaaagat atgccttttg gaaaaggagt atatgatgca
    10801 ttaaattggt ttgaaaatct aggtagagaa aacaatttca aagtcaaaaa tatagataat
    10861 catgctgttc aaattgaata tggaaatggt aaagagtatg tagatatatt tggacattgt
    10921 gatgttgtaa atccaggtga aggatgggat agtgaaccat ttaaactaaa tattataggt
    10981 gataaattag ttgctagagg tgtatctgat aataaaggac ctatgatagt gaatttttta
    11041 gcactaaaaa tgataaagga tttagatatt aatttaaaaa gaaaggttag attaatagct
    11101 ggaggaaatg aagagagtgg atttaagtgt ataaagcatt attatagcaa agagccatat
    11161 ggtgtatgtg gatttactcc agatgctaaa tttcctgtat taaatggaga aaaaggtgga
    11221 gcaataataa agttaatttc aaatatagac gataaatctt tatatataag tggtggaata
    11281 gagtttaata caataccaga taaagtttat ataaagaata tagaaaaatt gggtaaagat
    11341 aatatatgtt ttgatattaa caatatatcg ataaattatg ataatggcaa ttatattgtg
    11401 catggaaaag gtggacattc atctaaaccc gaaaaatcaa taaatcctat attagctaca
    11461 attaaacttt tgtcagaaaa tattgatgaa aaatggacaa aagatttata taagttgata
    11521 aatcaagata atataaatgg aaacttattt ggtttaaata ttgaggggaa atgtgggatt
    11581 ttatcaatgg tgccaacaat aataaatata gtagatggaa aattagaagt agttttaagc
    11641 gttagatatc cagaaatatt aaccattgaa gatataatga aaaaatttaa tttgtatatg
    11701 gagcaaaata atattaataa atttgagctt attggggaaa atttaaagca agcaaattat
    11761 atagatagaa attcaaaact tgtaagaagt ttgcatgata tatatataaa gtattctgga
    11821 gatttaaaaa atgatgttag agtaactagt gcaggaagtt atgcttctga aatgaataat
    11881 tcagtaatat ttggttgtga atttcctgat ggtagctttg gtaatgtcca tagtgcaaat
    11941 gaatttgcta gcttagatag acttatagat gcaataggta tatatgcaga ggcaataatt
    12001 acattatgta ataaaatata aatacaaaat gtcccttata tagacatatt atatatacaa
    12061 ttaaaatttt acttgatata taattgaaat caaagagaag gtgatacaat gataactaag
    12121 aaacatgcag taatagtaaa aagtttaagc aataaagacg gatatatgac atcaaatgaa
    12181 ctagctatca aactggatgt gtctactaaa actattaaaa gatatatagc agatttaaat
    12241 agtgttttaa gcaagtatga tttggtaata gcttcttcta gaggtatagg atacaagtta
    12301 agcggttcta aaaacaatat agccagagct gttaaagaag caaataaata tattgatggt
    12361 tttttagatg attctgaaga gtcaagaatg tcaaatataa tatgtatgct tattaataga
    12421 aattatatga gtatagaagc aatggcagaa gaattaaatc taagtatagc cgctataaat
    12481 aaattgtcta gtaagcttaa aagaaaactt gaaaagtatg acttagttat aaaatctaaa
    12541 ccttactatg gaagctatat atatggtgaa gaaattaata taagacaatt aattactgac
    12601 tatgcaataa aattagatga aaaaagtaaa gtgaaggtgt ttttagatga cattagtgaa
    12661 aacgatatat actgtataga aagtatttta gaaaagcacc taagaaaaaa agatacaata
    12721 atttcagata aagattttaa tcttttgctt tcaaaaataa tcgtttctgt atttaggagt
    12781 aagagaggtc atagtaacaa tataaactta atggatacaa gttatagatt tcataattac
    12841 tactttatag aaagcctaat gaaagaaata tctgataaaa ttggatttaa gcttattgaa
    12901 gatgaagtaa tttatatttc taattactct ggtgtaatag cctataaagg tacacaagga
    12961 agaaaaaaca caagtgaaat tgaggaacga ataagtagtg ttataagtag tgcacttcaa
    13021 gatatattcc ttatttcggg tagtgattat acaaaagatg aagagtttat ggttgcaata
    13081 tccgaccata taaagagatt tttaaataga gctaaagcta atataaaatc aaataatcct
    13141 ttacttcatc aaataaaaga gaagtttcct atagctttta atctagccgt gtttttatca
    13201 aataaactag aagctgagtt taatttaaaa cttgatgaag atgagcttgg atatatagct
    13261 atccactttg ctgcttcaaa tgaaagaatg aaaaaaaata ctagtaagaa aatttgtata
    13321 gtttgtcatt atgggatagg aacaggacaa cttatttctg aaaagctaaa acagaatata
    13381 agtgatttaa gtgttgtagg tgtttatcca gttagatatc tcgatatggc tataagtcaa
    13441 gatgtcgatt tgatagtatc tactgttgaa ttaaaaggat atgaaaaacc tgtcttatat
    13501 atagaaaata tatttgatga tagcttaatt gaaaacgtaa acaaagcctt ttatgaaaag
    13561 gaagaaagaa gaaagataat aagtaacatg tttgatgaaa aagctttttt tagtataaag
    13621 gctaacacta aagaagaagt tatactttca ctttcaaata aattaaaaga aagagacttt
    13681 atagaggaaa gttctataaa aagtataata gatagagaaa atatttcatc aactgaaata
    13741 ggaaatctag tagcaatacc tcacacaata gtgaagggag ataaaaaatc tataatagga
    13801 gttggaatac ttgaaaatcc aataatctgg gataaacaag aggttcagct agtatttatg
    13861 gtatttttta atactaaaga aaagcataat ttctcgatat ttaaatacct ttataatttt
    13921 ataaaagatg aaggaggagt aagaggaatt attaaaatat gtgactttaa taaattaatg
    13981 gcactgatag gtaattaagg aggaaatcaa taatgggaac aaacatattt aacaaagaat
    14041 atgtattttt aaatgttgat gctaagtcaa aggttgaagt tttaaaattt atatcaaaaa
    14101 aagctaaaga cttaaacttg gcagaggatg aaagcttagt atatgaaggt ctaatggcta
    14161 gagaagaaca atttactaca aatttaggag aatctatagc aatacctcat actaaaaatg
    14221 atgcaattga aaatccagca gtagtagtat tgaagtttaa tgaagatgtt gtttggaatg
    14281 aaggtgagga taaggttaaa ttagcaataa gcttacttat gccaggaaaa agtaaagaga
    14341 atattcatct taaattatta tcatctcttt caagaaaatt aataaataaa gaatttaaag
    14401 attctctttt aaaaagtgat aatattgaag aaatatctaa cttaataaat gaagctttag
    14461 gtttataatt aaacactaag gttataaaat aaaaaaataa ttaactaaaa ataggaggaa
    14521 tttgaaatga ataaaaaatt agtagcatta tgtgcttgcc caatgggact agctcacact
    14581 ttcatggcag cagaagcaat agagcaagca gcaaaggcat taggttatga agcaaaagtt
    14641 gaaactcaag gtgctgatgg tgtacaaaat gagttaacaa gagatgatat attaggagca
    14701 actatgataa tacatgcagt agcaataaca ccagaaggta tggaaagatt tgatggttgt
    14761 gaagtttatg aagttgaatt acaagaagct ataaaaaatg cagaaggtgt tataaaagaa
    14821 atagaagaag atttaggaat ataataaatt tattgtggag gaaatagaat ggcaattaag
    14881 aaaaaagtta taaacagttc aggtgcttct acaacaggag gaaataatgt aggaaaatct
    14941 actccatcta gtccaactca atcaaaggga aatggaaaat ggaaagaaat aagtaaacat
    15001 ataatgacag gtatatcata tatgatacca gttttagtaa tgggtggact tataggggcc
    15061 ttatctcaat taataccata tgctatatta ggacttgacc catctgtagg tatagttgat
    15121 gctatgaact caggagaatt tacaggattc aagcttagtc ttttaaatat agcacaatta
    15181 atgtcaaatt tcggatttac tttgtttgga tttgcaatac cactgtttgc agcattttgt
    15241 gcaaactcta taggtggaaa aacagcttta attgcaggat ttataggtgg atatatagct
    15301 aataaacctg taggtgtacc acaattcgtt gatggacaat ggactgaagt tgtgccagtt
    15361 gcatcaggat ttttaggtgc aatattaata gcattcataa taggatattt tgtaaaatgg
    15421 ttaaataaat ctataaaagt ttctcataac tggttagctt ttaaaacaac atttttaata
    15481 ccactaatag catcattagc gtgtatggta ttgatgatat ttataataac tccatttggt
    15541 ggattaatta atgaaagtat gaagaacttc ttaacagcag caggagcagc tggagaatat
    15601 gtatatgcaa cagctttggc agcagctaca gcattcgact taggaggtcc aataaataaa
    15661 gcagcaggat tcgttgcact tggtttaact acagagaatg tacttccaat aacagcaaga
    15721 acaatagcta tagttgttcc tccaattgga ttaggattaa caacattatt agataaaaga
    15781 atagtaggaa gaagagtata tgatagacaa ttctatcaag caggaaaaac ttctatattc
    15841 ttatcattta tgggtatatc tgaaggagca ataccatttg cacttgagag accaggtttt
    15901 gttatacctt taaacatagt gggaagtgta ataggagcta ttacaggtat aattctagga
    15961 gcaattcaat ggttcccaga gtcagctata tgggcatggc ctttagtaga caacttattt
    16021 ggatatataa taggtatagc agttggagct atattcatag cagtaggaaa tatattctat
    16081 agaaataaat taataaaaga tggtaaactt gttgtagatt atatcgacta gtatttaatt
    16141 tttactatct ataaataact ttggctggta atatggtttt aatcaatatt accagcctta
    16201 cctatatgat taattaaagg cggtggagat aagatatgaa taagatagaa aaagtaagag
    16261 agtattttat agaatatgat atagatggat ttcttataaa tagtcctaca aacaagtttt
    16321 ttgttggaaa tttatttagt tcttcaggtt atgtttttat aacgaaagaa gcacaatata
    16381 taatagtaga ttttagatat tttgaggaaa ttaaaagaaa atctagtttg tttaatgtag
    16441 tattaatgga caaaactaga actcattttg atataattaa tgatatttgt agagagcaga
    16501 atataaagga aattggattt gagggaaatg aagtatcttt tgatttatat agaagtatga
    16561 gtaataagtt aagtgcaacc ttaaaatcag ttgatttatc tacattaagg gaaataaaaa
    16621 atgaagacga aataaaatat ataaaaaaag cttgtgaaat agttgatgct acattttatc
    16681 atataataga ctttataaag gttggtatga ctgaaaagca agttgaaaat gaaattgtta
    16741 gagttataaa agatttaggt ggtcaaaaag aatctttcga tacaatagtt gcatctgggc
    16801 ttagaggagc attgcctcat ggaaaagcaa gtgaaaaagt tatagagtat ggagattttg
    16861 ttacttttga ctttggtgct aagtataata attattgttc agatattact agaaccattt
    16921 gtatgagtac tataaataaa gagttagaag aaatatataa tatagtaaga aaagctaatg
    16981 aagagtgtat aagggtttta agaccaggaa tgactactgg agagatagat aaagttgcaa
    17041 gagatataat tggttcatat ggatatgccg ataattttgg tcataatctg ggtcatggtg
    17101 taggaattat ggttcatgaa tatcctgcat tagctcctga atcaaatgaa gtattaaaag
    17161 aaggaatgat tgtaactata gagcctggta tatatgttcc aagtcttgga ggcgtaagaa
    17221 tagaagatga tgtacttata acacaagatg gatgtatgag attgactaca tctactaagg
    17281 atttaatagt tgtcagttaa aaataaatta ggtaaatatt ttataacaat aagaaaagaa
    17341 gaggtaaatt taaatgtata attttgatga taaaccagat agagtaagtg aaaagtgtag
    17401 aaaatgggat ttaaatatta taagagataa atttggagat attagagaag attttgtacc
    17461 aatgtggatt gctgatatgg attttaaaat accaacagaa atagaaaaca aatttattga
    17521 agctgtaaga agaggtgtat ttggatatac atactgttat gatgaatttt atgattcagt
    17581 tattggttgg caaaaaaata tgcatgaggt acatgtagaa aaagaatgga ttacattaac
    17641 ttatggaact gtatcaacac ttcattatgt agtacaagct ttttgtaaga aaggtgatag
    17701 tataatatta aacacaccag tttatgaccc ctttgaatca tcagcaaaaa aacagggtgt
    17761 aaatgtaatt tgtaatacat tagatgttgt aaatgataga tattatataa attttgataa
    17821 attagaagtt caaattaaag aaaataagcc taaattaatg atgttttgta caccacataa
    17881 tccatctgga aggatatgga caattgaaga aatgacaaga gtagcaacta tatgtaaaga
    17941 aaataatgtt attttggttg cagatgaagt acatgcagag catattcatt atggtaaatt
    18001 taactcaata cttaagattg aaaaggagct tttagaaaat gttattctat tgacatcacc
    18061 aaataaagga tttaatcttg gtggactaaa gacttcttat tcaatagtta taaataagga
    18121 tataagggac aaatttagaa ataaactaaa gcaaaattct ataacttctc caaatgtgtt
    18181 tggaataata ggattgataa ctgcttataa tgaatgtcat gagtggttaa gtggtgtaaa
    18241 tgaatatata aaatcaaatt atgaactttt agaaacttgg gtaaataaat ataataagat
    18301 aaaattgatg aaaatggaat catcttactt agcttggatg aatataagtg gattaggtat
    18361 aagtgcaagt gagtttacag ataggttggc tatagataca ggggtattat tagaagatgg
    18421 aagccatttt gtaaaagatg gtgagaagta tgttagaata aacttaggaa ctcaaaagga
    18481 aaatgtaatt gaagctttaa atagaatgga tttattttta aagtctttat aaaaatttgg
    18541 ttgaattagt attttatata aatttaaact attaatatgg tatatacata agtagaattt
    18601 gttttagatt acttaagtat ataccatatt ttatataaaa attaaatttg aattagttat
    18661 gtgataacta tttaaattca tatgtatttt gtatgcataa cagatatagt tattatttat
    18721 ttaatttttc aatagaaaaa gatttatctg taagtttaaa attatctttt atacccttag
    18781 ttaagtacac tttcttatcc ttcgttatga atatagcatc tacattatca attttattaa
    18841 ttaaatccat acctttttca agaccaagtc caaatgtaga agtggatagg gcatcacaat
    18901 taatagattt atcagaaatt attgtaacac tacttaaatt gttttcaaaa ggataccctg
    18961 taaatggatt taacatatga tgatacattt ttccgtcttt ttcgataaat ctttcataaa
    19021 ttccagaagt aacaacagat ttatttgtgg tttctatgtt tcctatggat tcgccatttt
    19081 tattagttgg gtcttgaata cctattttaa aaggtttatc atttttacca tcttctaaag
    19141 taaatatatt tcctcctaag tttactaaac ttttttttac gttctctgat tttaaatatg
    19201 ctacaatttt atcagcagca tatcctttag ctatggcacc taaatcaatt ttcatatttt
    19261 ttttggttaa ttttatagat ttattttttt catccaatat tatgttctta taatcaataa
    19321 gaggcaaaag tttttgtatc tcagaatcac ttggaacttt agctttatct gttccaatag
    19381 cccataaatt tgataatgga ccaattgtta tgtcaaaata tccatctgat aatttggaat
    19441 attgtatagc ttcttttacg acgaaaaatg tatcatctga aactctgaca aatttcttac
    19501 cagcattatc atttatttta gaaacatcac ttcctggtat atgagtgctc attttgttgt
    19561 caatatatct taaaattgaa tcacattcat ttaagatttt atcacttttg gattctctta
    19621 cattaaatac agtaacttcg tttactgtac caaggtagta gctggtttta ctatatttag
    19681 tattctggct tgaactccta aaaaatgaaa ataaaattaa tgctaaaatc aatatcacaa
    19741 aaataaatgt gatttttttc ttattcatgg tatattcctc ctatgaaatt gtgttattat
    19801 tttattataa cataatttca taggaggaca aaaaatgaag aaaaaagaca ttcttcttat
    19861 tttaggcatt ttggttgtta ttacagcatg ttatgggata ataaatgtta taaattctaa
    19921 aaatgctgga aatattgaga tttatgtaga taataaattg tataaaactg tttcaataaa
    19981 tgccaaagaa gaatttaaaa tagagaatag aggggggtat aacatagtaa agattcatga
    20041 taaaggggtt gagatagtag atgcttcatg ccctgataag gtttgtgtac atactggatt
    20101 tataaacaag ccaagtcaaa gcatagtttg tatacctaat agagtgagca taaaaataaa
    20161 tacaaatgaa aagaatgata atcaagaaga tataatttct aaataaaata ataaaaataa
    20221 ttgaaaggaa ttggtgataa ttatggttaa cttaggaaac gattgggatg aattattgaa
    20281 agaagaattt gaaaaagatt actacttaaa tttaagaaaa tttcttatag aagaatataa
    20341 aacacgtcaa attttcccaa atatgcacaa tatatatgaa gctttaaaac acacttcata
    20401 caaagacaca aaagtcttaa tattaggaca agacccttat catggagata atcaagctca
    20461 tggtttagct ttttcagttc aaccacaagt gaaaactcca ccatctctat taaatatgta
    20521 taaagaatta aaagatgatt taggttgttt tatacctaac aatggatatt taatgccttg
    20581 ggcagaccaa ggagtgttac ttttaaatac tgctcttact gttagagcac atgaagctaa
    20641 ttctcataag aacaaaggat gggaaatttt cactgataga gtaatatcta ttttaagtga
    20701 gcgtgaagac ccagttattt ttgtgctatg gggaagtaat gcacgtaaaa aagtagagtt
    20761 gattgataca agtaaacatt atattttgga agcacctcat ccaagcccac tgtcagcaag
    20821 taaaggcttt tttggatgta aacacttctc taagataaat gagattttga aaaagctagg
    20881 aaaagaacct attaattggc agatagaaaa tatataaaag taaaaggtaa caaaatgata
    20941 acaaaacaac aataaacaat gacattgttg taactaatag aaatatgctt aaatagttga
    21001 aaatactata tatgcatatt tatatttttt tgtcaagttg acaactcttg aaaaaatagt
    21061 tacaatttag ttacacaaaa aacacaaata aaaaagaaac ttacaaagca gaattttatg
    21121 aagcaaaata tatagtgtaa aaaatcttag ggaggaaatt ttatatgaat tttaaaaaat
    21181 tgagtaatgc taaagtctcg gtaatggcag ttgttttatc agttggaata ttatcgggat
    21241 actctgcgct taataaacaa gtaacattag tagtaagggg agaagaaaga actatatcaa
    21301 catttagttc taatgtagga gaccttttag aatctcaaaa cattagctat gatgcaaatg
    21361 atatagtaac tttagatgaa aatacaaagt tatctaatgg agataaaata gaagtaattg
    21421 atgtaaagga aaaaacagtt gtagaaacta aagacattcc atttgaagtt acagttgttg
    21481 aagatggctc tttattaaag ggtgattcta aggttaaaga agaaggtaag tctggtaaaa
    21541 gtgaattaat atataaagaa acttatcaca atggaaagaa agtagaaaag gaatttgtga
    21601 aaaaggttgt taaagctgag ccaataaata aggttgtaag caaagggaca aaggtagaaa
    21661 tgcaagtagc ctcatcaagg ggagaaagtt caagaagaat ggcttcatca tcatctagta
    21721 gcagtagtag taacaatggt aaaaatatga gagtggtagc aactgcatat actggtcaca
    21781 gtataactgc aacaggaaca aaacctaagt ggggaacaat agctgtagac cctaaggtta
    21841 taccatatgg aactaaagtt tatatacctc aatttgacat ggtgtttact gcagaagact
    21901 gtggtggagc aataaaagga aataaaattg atatctacat gaatgattca aaaacagttt
    21961 ataactgggg aagaaaaact atagatatac gcatattaaa atagttaaat aatttattaa
    22021 gatagttaaa taatttatat ttgtactgga aattatattt tgattatata gagaagaaac
    22081 tctttataga ttattaaaat ataatttcca gttttttatt ttggatatgt aataaaacag
    22141 gtaaatttta accatgtata gataaaagct atttaaatta taatttacta tcagataaat
    22201 ctataaaaat tgttgcaaag agtcaaaata tatgatatta tagatttcgt tgagaaggat
    22261 ttcattatgg gagtggatgg gtgccggtgt gccctctagt cttcaaaact agtatgaggg
    22321 gttaagagcc tcttaggtgg gttcgattcc cacgcattcc cgccaatatg taaaaggtat
    22381 cttttaatta aaagatacct ttttaaattt attttaagat aatttatttt taataatata
    22441 taatattttg tattatataa tgtgaatttt ctaatactat tatatattta gataaaaatc
    22501 taggagtgtg gaaagatgga aaataatttt acaacgagaa ctagttttct agttggagat
    22561 gatgggatag aaaaacttaa taattcaaat ataatagtat ttggagttgg aggagttggt
    22621 agttttactg ttgaagcttt agcaagagct ggagtaggca acattactat tgtagatttt
    22681 gacgatgtag atataaccaa tataaataga caaataccag ccctacattc aactgtagga
    22741 aggtacaaag ttgatgtcat ggaagaaaga atattagata taaatcctaa tatcaatata
    22801 aaaaaaataa gaagtcttta taataaagat actagtgatg aaatactaac tgaaagatat
    22861 gattatgttg tggatgcgat agacatggta agttctaaaa tacatctaat tgaaacttgt
    22921 gaaaaaaaag gattgaaaat aataagttct atgggtatgg gaaataaatt agacccaact
    22981 aaaatagtcg ttacagatat acacaaaaca agtacttgtc ctctagctaa agttatgaga
    23041 aaagaactta gggatagagg tatcaaaaag ttaaaagtag tatattctac tgaacaacca
    23101 atagaactta agaagaaagt aatgaatggt agaaaagtaa cacctggtag tgtatcattt
    23161 gtcccatctg taggaggttt aataatcgca tctgtaatag taaatgaact attaggacaa
    23221 taaattttaa atattttaat ttataaattt tatatacaaa cttgaaggct agaagcatta
    23281 atataaaaca attttatatt catactttta gcctaattat tttatattac cttattttat
    23341 gtagttgtag ttttattttt tagattatgt atctatttca taattatatt ttgtaaatat
    23401 aatttctcca tcactataaa aaacaatatc tcctattcta tctgttctat aaattgatac
    23461 atggttttgt tttagattat caataacctt cctatgtgga tgaccatatt gatttttata
    23521 tccacatgat attgcagcta tatccggact aatttttttt ataaattcta aagatgatga
    23581 agaattactt ccatgatgac ctatttttat aaaattaata ttatttaatt taaaagaatg
    23641 taaaatttct ttttcatttt cttcttcagc atcacccata aataaaaaag aattatcgtt
    23701 aaaagttaat ttgaaaacta tagaatttaa attagattct tcttgtatat atgatggact
    23761 taatacatat atatttatat tgttatctat gtttaaaaca tcatttttat agagatgctg
    23821 aatagataag tttttatctg tacaagaatt tattagattt tgataagcct cactatcagt
    23881 tgattgttca ggcatatata tggagtttac attaaatttt tttataacat tatcaagact
    23941 tcctatgtgg tctgaatctg gatgtgttgc tattataata tcaatagttt ttattctttt
    24001 ttgtctaaga taactaataa ttatattttc gctatcttca tcacctccat caattaatat
    24061 gttcttattt gtgggtgttt gaactaaaat actatcacct tgtccaacat caatcatatg
    24121 tatagatagt agagattttt tgtcacagcc gattaaaagt gatattataa taagtattga
    24181 taaaaaaatt ttatttctca ataaaaaatc actccattca ttacatgttt atataaattt
    24241 atgatgggta atataagata tatatgactt attatatttt atattttaaa caatataaat
    24301 gaaattaata ccataataaa attggagttt tttatatata caaaataaaa tttttaaaat
    24361 taagcaataa aaaaggtagg aggattttat gtttttcaaa gatattgaac taaattcaaa
    24421 aaaagagtta gacccatatt ttgatttagt agattatgaa gcttgtgagt actgttttag
    24481 tactttatat atgtggcaac atgtttataa gacaggatat tatataggtg aagattttgc
    24541 cgttttagtt ggggaatacg aaggagatag cttctcaata ttaccacttg ctaaaaaaga
    24601 taaacttcca gaagttgtag attttgtcct tgaatatttt tctaaaaata ataaaaaaat
    24661 atacttaaga ggtattacta ctgaagtagt agagttttta aaagaaaaat atccaggtag
    24721 atttgagtat atagaagaaa gagatttatt tgactatatt tatgatgcag aaagtttaag
    24781 aactctagct ggtaaaaaga atcaaaagaa gagaaaccat ataaactatt tcttaaaaga
    24841 gtatgcaggt agacatgaag ctaaattatt agataaagaa aactttgatg agtgtttagt
    24901 actaatgaag gaatgggaaa gtaataagga agaaaataat gaatttgatg aaagtatgga
    24961 tgatgaatta ataggtatca aaaagatatt taaccattat gacatattaa aagataaggt
    25021 aaaagtgttt ggtgtttatg tggatggaaa attagaagct tttagtatag gagaactttt
    25081 aaatccaaat atggcattga ttcatataga aaaagctaat cccgatataa gaggtctgta
    25141 tccatttata aatcaacaat ttttggtaag tgaatttaag gacgttgagt ttgtaaatag
    25201 agaagaagat ttaggtattg aaggattaag aaaagctaaa ctttcttatc atccttgcag
    25261 atttgttgaa aagtacagtg ttagggaggc ttaatagact atatggagat taggtatgct
    25321 aaagaagaag agatagaaag tataaaagag atttggagtt attgttttaa cgatacggag
    25381 agttttatga agtactattt taatgataaa tataaatctg aaaatactgt agtagcttta
    25441 gatgaaggaa aaattatttc ttctcttcag ctaaatcagt ataagttatt actaaatagc
    25501 aaggtatata atacatcata tgtagtagga gtttctacac ttccagaggg aagaggagct
    25561 ggatatatga acaaagtaat gaaatttact ttaaacgaat tgtataaaaa agggcaatta
    25621 gtttctatat tgatgcctat tgactataga ctttatagaa gatttggata tgagcattgt
    25681 tacgaccaga ttgaatatac tattaacaca gatgatttaa aaaattttaa gtcaagtgga
    25741 aaaatgataa agtctgattt atcacaaatt gatgatttga tacgaataga tagaactttt
    25801 ttaaatgagg ttaatggtaa tgtgttaaaa gatgaacatt attatgaaaa cttatttaaa
    25861 gagattcaga gtgaagatgg atttttatac attcatgaag gaaatgaaaa agatggatat
    25921 atagtttatt ttcttcagga aggtaaaatg tttgtcagag agttatttta taaaaatata
    25981 gatgccctta aatcaatgct taaatttata tataatcaca atactcaatg taagatagtc
    26041 actatatcta ctcctacaat agataagata agatttatat tagacaaccc taaagatagt
    26101 gatataaaaa taaaaccttt tatgatggga agaattataa atgtaaagaa gtttattgaa
    26161 gatatagata ttgagaagga tattaatagc tcatttaatt tattaataga agacaagttt
    26221 atagatgaaa ataatggtct atttaaaatt tctatacaga ataagaaggt atctgtagag
    26281 cagttagata aaaaaggtgc agaaaagcct caagaagatt ttgacattaa attagatata
    26341 aataccttaa cacagttatc attttcttat atagatgtaa atgaagccat tttcttaaat
    26401 gatataaaag atgtttcaga agaaacacta gaaacactta attgtatttt ttctaaaaaa
    26461 aatacctata taaatgaata tatataggta gagtacgtta aaagatttta aaaatctttt
    26521 gacgtatttt tttgtttaga taacaattta tcttgtttaa aaattttcta tttgaaaata
    26581 atctaaataa tatactttta tatgggagtg attataattc aaataagaag acctttatta
    26641 attatattaa tgtttgtggt agctatatca tttattatta caaataacac aaaaaacgac
    26701 gaagaactta atgataagat aataactata caaggagttg taaaaggaaa aattgaaaag
    26761 aaaagataca atcagtataa ggtaggagtt tttctaataa atgattatac aaaaaataaa
    26821 aatttgaaga ttggtcagaa ggttaatatt acaggtaaat ttaaaagttt agataaaatg
    26881 aagtatgatg attttgatta tggaaggtat ataaaaagca ctggatataa aggtatagtg
    26941 tatataaata actataaaat tattgataaa aataaacttt actctttaat agggaaaata
    27001 aagttttata ttagtaagac ttataggtac ttatataaga aaaactcaga ttttataaat
    27061 tctatattat tagcagaagt tgaaaatttg actgaggaac aaaaagaaat ttttaccaga
    27121 acgggaacta gccatgtaat atctatatca ggtcttcata caggaatatt atgtgtaatt
    27181 atatcctttc tattaagagg tataaataaa ttgtataaat tattaatact gtcgattttt
    27241 ataactttat attgtataat ggtaggtgcg tctccatcca taattaggtc tatagcattt
    27301 gttatgattt tttatttatc aatatttatt gatagaaaga aagatggaat atcagcattg
    27361 tctctaattg ggataatttt aattatgaat aacccctatg ttatatataa tgtaagtttt
    27421 caattgtctt ttttagctac actttctata ttaatattct ataataaaat aaatagtatt
    27481 ataaaattaa gtatggtatc attgacaata tcatcaaata tattaacttt acctataatt
    27541 tattatacat ttaaaggtat accattgctt tcaattatag gaaatttaat tatagtacca
    27601 tttgtgggag taataatgta tttaagtatt gcaagtttaa tagtatttaa agtaagtgta
    27661 gtaatagcta aaattatatc tttttttaat agcatattga tagaaagcat atttttttta
    27721 ctagaaaaaa tcagcaattt aagttttgca tacactaata tagaaagtcc taaattttat
    27781 attgttgtta tttattacat aggagtgttt ttttacatat tttacataga aggaaaagag
    27841 ataaaggagc aagagaatga attacaagga tattataagg aatgtaaaag agaaaaactt
    27901 tgaaaaaatg tatctttttt atggaagaga gtattattta attgagaatg caataaaagc
    27961 atttaaagat agtctaaacg agggaatgct tgattttaat ttagatataa tagatgggaa
    28021 ggaaatagtc ttaaatcagc ttataagctc tatagaaaca cttccattta tggatgatag
    28081 aaaaatagta ataataaaag actttgaact attaaaagga aagaaaaaga attttacaga
    28141 tagtgatgag aaatacttaa tagagcattt agacaatatt ccagatacta caactatagt
    28201 ttttgttgta tatggagatg tagacaaaag aaaatcatta gttaagaaaa tagggaacaa
    28261 tggaattgtt tttgattgtg ataagctttc tgatatggat ttatttaaat ggataaaaaa
    28321 atcttttgct ctaaatgatg ttataataga taattctcaa atcatgtatt ttattgaaca
    28381 agaaggatat agagataaga gtagtgaaaa gacattatct gatttagaaa atgaaataaa
    28441 taaaataagt tcctttgttg gtaaagggaa taatgtaacc aatgatgtaa ttgataaatt
    28501 atctcaaaaa aaagttgaga atgatatatt caaattaatc gattatattg gagaacaaaa
    28561 tgcttctaat gcaatgaaaa tattaaatga tatgatacaa gaaggtgaat ctgtattagg
    28621 tatattttct atgatagcaa gacaatttaa aataattatg caagtaagac agttacaact
    28681 tgatggatat tcaacaaaac ttatagctga taaattaaaa atgcatcaat ttgtcgtagg
    28741 aaaagcatta aagcaaacta aaaatttttc tgatgatata attgtggaaa tattaaacta
    28801 tatattagaa agtgattaca aaataaagac aggtcttata agagatactt tagcagttga
    28861 aatgttggtt agtagatact gtaaaagaga agcaatttaa aaaatctata tattaataaa
    28921 aaataaatag caagatatgt tatagttcag agtagatgta agctaaatta agtttgatat
    28981 acaatcaact ataatatgtc taactattta ttttttattg tataaatttg caaaataaaa
    29041 aacccctaca taggggttat tatatataag aaatataaac tggtaattaa gcgttcatac
    29101 cgtttaattt ttgagctaat ttagctactt ttctagctgc agcattctta tgtatagttc
    29161 ctttagaagc tacttggtat attctttttt gagcgtattg gaacttagca acagcatcct
    29221 ctctgtttcc agctgcaaca gcatcttcga atcttcttat tgcagttttt atttgagatt
    29281 ttctaactct atttaaagca gttttctttt cgataacact tattcttttc ttagctgatt
    29341 ttatatttgc caccgtgttc acctccctgt atttttttat catattcatc gtcaacatat
    29401 tagattttaa cagaaataaa tctaaaaatc aaggattatt atttatatat tttaataatg
    29461 ttttttattt tgatttttta gactgtaaat tgtgtataaa taatttgcaa tatcttgttt
//
LOCUS       DAEPXF010000029        29439 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain HB27
            SAMN13376389-rid12479423.denovo.031, whole genome shotgun sequence.
ACCESSION   DAEPXF010000029 DAEPXF010000000
VERSION     DAEPXF010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13376389
            Sequence Read Archive: SRR10518432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 29439)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29439)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001069624.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001069624.1
            Genome Coverage       :: 315x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/15/2021 01:54:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,165
            CDSs (total)                      :: 4,076
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 89
            rRNAs                             :: 10, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 10 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 19 of 69
            Pseudo Genes (incomplete)         :: 44 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 10 of 69
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29439
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13376389-rid12479423.denovo.031"
                     /strain="HB27"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="China: Hebei"
                     /collection_date="2017"
     gene            33..479
                     /locus_tag="KRL67_001178"
     CDS             33..479
                     /locus_tag="KRL67_001178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HBH1522075.1"
                     /translation="MDFKNLQYESLSYIICNLQKNFKLYCEKCLKPYKLTNGLYFYLI
                     YINKNRNCSLNDVSTEFEVDKAHTTRTISRLEQDGYIEKIQNPNDSRAFQLRVTDKGE
                     EVLGDIKNIFSKWDNRIKKEFSDTEYKELVKNLHVVKDIKTAVEEE"
     gene            481..1575
                     /locus_tag="KRL67_001179"
     CDS             481..1575
                     /locus_tag="KRL67_001179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavin oxidoreductase"
                     /protein_id="HBH1522076.1"
                     /translation="MYCNLLKPIKIGNLELKNRVSFAPTSMGLKLEEKIKKFSDIAKS
                     GVALITLGDVSIRPSFHKIAISLSDEDGVMKYKKIVDEIHNSGAKVSAQLFCSDYDVN
                     LIKDTMKMGITSHDEIKKIMNDGVKDYITNMPKEEIKNIINLFKVTALNAKKAGFDMI
                     QIHGDRLVGSFSSSIFNNRNDEYGGTCENRSRFASEIISSIRNEVKDIPIDYKFAIRQ
                     ENPHYGNAGVLLSEVEYFVKKFESLGINSFHVTLANHSKLEDTIPTNNHPYFKDEGCF
                     LYLADEVKKHTNLPVCGVGKLSSPDFIESIISNNRVDMISMSRQLLADSNWLQKVKDG
                     RVDEIKKCCYCNKKCADALQTHSQFGCILD"
     gene            1663..2193
                     /locus_tag="KRL67_001180"
     CDS             1663..2193
                     /locus_tag="KRL67_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HBH1522077.1"
                     /translation="MKSLILYSSLTGNTKKIACSIYEAIQGKKEIKNISELVDDSIDY
                     ENYDIVFLGYWVDKGICDKNSKQVLENIHNKKIALFGTMGASTKGSYGASIIEKIESI
                     IPKDNEILGSFICQGKIAEGLKVKYKEMLKLSPDNEHIRQQLNNHEESQSHPDEQEIY
                     EANVFAKNMMIKASTM"
     gene            2520..3353
                     /locus_tag="KRL67_001181"
     CDS             2520..3353
                     /locus_tag="KRL67_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HBH1522078.1"
                     /translation="MTIKEVEEKTGLTRSNIRFYENENLIKPLRNERNGYREYSDENV
                     EDIKKIAYLRTLGITIDNIRNIIFQKVSLVEVVEKQEKVLETKISDLENAKTMCNKML
                     ESDELSYLNLKVEQYITELPEYWNKNKPVFKLDSVSFLYIWGGKITWGIITIACLLIA
                     ITVFPKLPPQIPIQWNNGVANYVDKKFIYAYPVICIVFRILLRPFIWRWLQIHAFYSD
                     TIADYLTNFMCFVALSIEIFSILFIYNIVRHVTVIIFIDILVFIALLIIGVYKLFKKY
                     N"
     gene            complement(3602..4978)
                     /locus_tag="KRL67_001182"
     CDS             complement(3602..4978)
                     /locus_tag="KRL67_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH1522079.1"
                     /translation="MKYKIGFLTTSYFMSDKFKKVLKELNDICEITFIELKGENPFNN
                     LPNIYYEQVDNFDGFVCSGIVPYSELIINIKDIKVPLNFLKLDERDFYKYLFKLLNSQ
                     KDIDFTKSFMDFLREDNNYYDIYSLIDSNKCPYTIKDFNIPQPVYDFKKLNDKLLEIH
                     LDLLRDDKINLSFTRNYIISCELNKLGYDCIYMVPSTESILNTFKSLVNEITLQNLDK
                     NKSATCIVTVNSSEYINEDLIFKNDEIQNQIYNTILNSLSRHGFYGVQVKKNDMKIEI
                     HTTKEMLNNMTNNYTDFFISEDLKEIKYSLNIGWGIGNTNIHAEQNARQANGKSASLN
                     GNCTFIVNDTNDTIGPLYSNKNSNNIEDSSIANKVASFIPLSNTNVSKIMCMINDRNS
                     NNVSAEILADYMNITLRSANRILSILYKAGIATIVNTKLDNQRGRPKKIYKVDFLSFL
                     NKMQKLNS"
     gene            5246..6418
                     /locus_tag="KRL67_001183"
     CDS             5246..6418
                     /locus_tag="KRL67_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HBH1522080.1"
                     /translation="MNTYLNEISKFVDENREEIVSLWKEIVNMESYTHCKESVNKLAK
                     RLKFEFEKEGLDCELVDVGDNGSTLIGTLGSNIDKKPIIFSGHMDTVFETGTFGENPF
                     KIIEGKAYGPGVLDMKGGIIISLYVIKALNKIGYKERPIKIVFSGDEEIGHKDSTGAD
                     VILREAKGGLCAFNMETGLVDNSLCVGRKGRIGCNIHVKGVETHAGNDFEGGRNAIEE
                     MANKILRIQKLTNLDVGTTVSVSVIKGGRVENSIPEDCSIKVDLRFEKVEEMENVKEQ
                     VREICKETYIEGTSTEVNFISEMMPFETTEDVMRFHTFVNEVSKENGFGELNAKRLGG
                     SSDASYLTIANVPAICSFGVRGEWNHTSREYAVVDSMFERVKLISTVILNLDKFEV"
     gene            6432..7079
                     /locus_tag="KRL67_001184"
     CDS             6432..7079
                     /locus_tag="KRL67_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH1522081.1"
                     /translation="MDVTAGDNKVTWKGYLSLFVLIVLFSGVFKDSQGFLKALDFANL
                     TGSFGTIADGVDFMGKGGTGAKDGFLFALTLIPTVCFAVGLIDVVESMGGMKAASKIF
                     NPLLRPLLGIPGIAGIAFVSSFTSSDVASIMTKELFESGEITDDERTIFVAYQYAGSA
                     VILNTINTQAPLLPIALLALGPIILIEIFCKILGANLVRLIIGVRNKKNKAKEAV"
     gene            7081..7590
                     /locus_tag="KRL67_001185"
     CDS             7081..7590
                     /locus_tag="KRL67_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjiG family protein"
                     /protein_id="HBH1522082.1"
                     /translation="MSQQNTGNAIVNSKPKKTAVEIFMGGAKKGFYIGVEQILPAMIM
                     GYVIVQFLQLTGLVDILGNIFGPVMGIFGLPGESVVVLIAAFFAKAAGAATAANLFAQ
                     GLITAPQATILIMPCMLMGTLVGHYARIVLVANASTKYRMLLLAVPIFDAIVGMLIVR
                     VLLTVMGLM"
     gene            complement(7977..9089)
                     /locus_tag="KRL67_001186"
     CDS             complement(7977..9089)
                     /locus_tag="KRL67_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HBH1522083.1"
                     /translation="MKISEVATPNFLLDLDQLEKNIDKIQQICTNNNKQLWPMLKTHK
                     STYIARLQKNAGASGFLVGTLDEGEALIKAGFEDIMLAYPYMGEVNINRIVTMASKIN
                     LICATDNIECAKAYSEAFSKSKIECKLLIIVDCGLHRFGVEPEKVVELATEISKLENI
                     KIVGISSHPGQVYGCSSPEGVPDVCVQEDSSMEKAYKALTDNGFNIEIVASGSTPTVE
                     AEAASKTITAVRPGNYVYYDAIQIGLGCATEDMCALTVVCTVISKNSSGYYLIDCGSK
                     CLGLDKGAHGNSSIVGYGRVCGHPELTIDSLSEEVGKIKVNGETDIKIGDRIRIIPNH
                     SCSTANLTSNLLGFRGDTIEKVIEVDIRENSKQIIL"
     gene            9387..9950
                     /locus_tag="KRL67_001187"
     CDS             9387..9950
                     /locus_tag="KRL67_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HBH1522084.1"
                     /translation="MLEVVNMRKVVLYIATSLDGYIAEENGSVSFLDEISSEFKNEQE
                     DDYNTFYDTIDTIIMGNSTYVQIANELSPNEWYYKGKECYVYSNTINGTTENVKYTNL
                     EPKKLIEQIGKEKEKKDIWIVGGGDIVKLFMKDNLIDDYYIYILPIILGRGISLFKSG
                     IDKTNLNFKKASNIGELVKLEYSKKQK"
     gene            10195..11922
                     /locus_tag="KRL67_001188"
     CDS             10195..11922
                     /locus_tag="KRL67_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003496244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBH1522085.1"
                     /translation="MKLILSYLKNYKLLIVLNVLAIFSFALVELGIPTIIAKIIDNGI
                     ANQNIAYIKQMGIVIVIISIIGVVGSILLGYCSAKVSTSVTRDIRNDIFEKSQEFSHT
                     EYNKFGISSMITRTTNDAFQIQQFVNILLRTALLTPVMFIISIIMTIRTSVELSIVLA
                     ISVPFIIIGVAIIAKVSQAISSKQQKGLDKLNLISRENLTGIRVIRAFGNDDYETERF
                     EKTNTYYANVSKKLFKLMSITQPAFFLLLNIAVLAVFWISSEKINIGELQVGQLVAFL
                     EYLFHAMFSIMLFSMVFIMYPRAEVSANRIKELLNEEPLIKNPENGIKDTENKGIIEF
                     DNVTFTYPDGEASVLKDISFTAKTGETVAFIGSTGSGKSTLINLIPRFYDVTEGSIKI
                     NGVDIREYDLKALRKKIGFIPQKSLLFTGSIANNIRFGKHKAGESELEYSAKVAQAYE
                     FISKKPRKFDELISEGGANVSGGQKQRLSIARAIIRRPEIYIFDDSFSALDFKTDAIL
                     RAKLKKETKDAIVLIVAQRISSIIDADKIIVLNEGQVVGMGTHKELLKNCEIYYEIAT
                     SQLKKEELE"
     gene            11922..13712
                     /locus_tag="KRL67_001189"
     CDS             11922..13712
                     /locus_tag="KRL67_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBH1522086.1"
                     /translation="MKKSKGFKKTIGRLLPFVKKYKFSFIIAIICIISAATMNALAPK
                     TEGLIITQLTKDVISIAKGVPGASVNFDYVTKILVILACIYFANAIFTYTSSFLLTNA
                     IQNTMRDLRNEVENKIRRLPISYFDSNSFGDVLSRISNDVDTISNALQQSFMQIVNSI
                     LVIILALSMMFTINIYMALIALFIIPISYFVSKFVVKKSQSRFSLQQNALGKLNGKVQ
                     EMYTGFNEIKLYGKEEDSIKEFKKVNQELCENGFKAQFISSMMNPMVSLVTYFGIAAV
                     AVVGSIYAVSGGITVGNLQAFVRYIWQINQPLSQMTQLSTVIQSSFAAIERVFEILDE
                     EEEIPDVENPVKIENVKGNVTFEHVNFGYGENSTLIEDLNAEVKSGQMVAIVGPTGAG
                     KTTLINLLMRFYDVKKGAIKIDGVDIRDMKRKDLRSMFGMVLQDTWLFNGTIFENIEY
                     GRFGATKEEIIQAAKVANVHHFITTLPDGYNMFLNEEASNISLGEKQLLTIARAFISD
                     PSILILDEATSSVDTRLELMLQKAMRNLMNGRTSFVIAHRLSTIRNADLILVMNNGSI
                     IEQGNHDELMEKGGFYEKLYNSQFADKESE"
     gene            complement(13874..14182)
                     /locus_tag="KRL67_001190"
     CDS             complement(13874..14182)
                     /locus_tag="KRL67_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH1522087.1"
                     /translation="MFNKIYEKKEFIVFQVKKGYVVYNTRKSFEEGHTHLKHFEAAKT
                     AIDLAINKKIPRSKDGYYLTSLIRISDDGYYIDKLSELLYVREQKGKKEKYCNSGYQM
                     "
     gene            complement(14444..14992)
                     /locus_tag="KRL67_001191"
     CDS             complement(14444..14992)
                     /locus_tag="KRL67_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH1522088.1"
                     /translation="MIFLNEDVVTNETIDKDYAIEEVRMACKHFGDLYFYFSKVLFEE
                     FGEDKTSEILRKVLFERSEERAIAMRERAHENGDELIADNIISTTDVPFLGWVPEFEE
                     LHCPYGASWLLRFDENPWFKKFASLYCDVTDTTVAEVFTGDTSHKITKNILWGDKSCE
                     RIYFHDSKVSEGKYTYGNKNCK"
     gene            complement(15094..15765)
                     /locus_tag="KRL67_001192"
     CDS             complement(15094..15765)
                     /locus_tag="KRL67_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBH1522089.1"
                     /translation="MATFKRLQEQAYDYLKELILSGKMIENEIYSETKLASEIGISRT
                     PIRDALQRLSQDGFIDIIPSKGFRIHQITANEIVEIFQIRSAIEGFCTFLITSQYKEP
                     RAVETISKLKHLLDKQKDILLGDKNLNSFAEYDTLFHTTIVSYAQNTEFDKMFNNYMY
                     RIKKLALDSLSHEGRLETTLKEHTDIFNNIANGCIEDIYRTTLIHMETPKYINLEDFC
                     NNKLF"
     gene            16161..17066
                     /gene="garR"
                     /locus_tag="KRL67_001193"
     CDS             16161..17066
                     /gene="garR"
                     /locus_tag="KRL67_001193"
                     /EC_number="1.1.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-3-oxopropionate reductase"
                     /protein_id="HBH1522090.1"
                     /translation="MEGYKMKLGFIGLGIMGKPMAKNLLKDGCNLLVYDINKSAVDEL
                     ISCGAKYASVLEMGQECDIVFTILPNGTIVQDILFGMDGLAKTLKEGSIVVDMSSVTP
                     TESILCANKLKDMGLEFIDSPVSGGEPKAIDGTLAFMAGGKEEIYKKVEPFFNIMGSS
                     SILIGDNGSGSVTKLTNQVIVNLTIAAVSEAFVLAAKAGADPEKVYKAIRGGLAGSTI
                     LDAKIPMIMNRDFKPGGKISINLKDIKNVMQTAHNLDVPLPMTSQLLEIMQTLKVHGH
                     LEDDHSGIAQYFEKLAGVEIKKHNN"
     gene            17202..18584
                     /locus_tag="KRL67_001194"
     CDS             17202..18584
                     /locus_tag="KRL67_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntP family permease"
                     /protein_id="HBH1522091.1"
                     /translation="MITGYGLLIAFVISIGILLVSIIKFKVNPFLALLITSIITGFMV
                     RMPINEISTTISTGFGNTLGSIGIVIGLGIIFGNILSESRATESIAKGLLAKTGEKNS
                     ALAVTTAGFLISIPVFMDAAFVIMMPIIKYVSRVTKKSIMVFVCALGVGTIVGHALVI
                     PTPGPLAVAANVNANVGSFILYSIIVAFPAALIGGWIYGKRFEKYPAYAIDENDREKN
                     LEQGKGSIKIEDDDSSKVPGFGISMFSLLFPILLILISNVFSMFLEKGSTMSGVLAFI
                     GDKNIAILLGILVAIGFLKKYINKPMGDVVIEAADSAGLILLITGSGGAFGSVINASG
                     IGNFLVDTMSGLSISVVVLGFLLSALLRISQGSATVALVTTSSILGPTILATGMSPVL
                     VGLAICAGGVGFSLPNDSGFWVLSRFSGLSVKDTLNSWTIGGTIAGVVAFIMVLLLSV
                     INGIIPLPGL"
     gene            18655..20091
                     /locus_tag="KRL67_001195"
     CDS             18655..20091
                     /locus_tag="KRL67_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
                     /protein_id="HBH1522092.1"
                     /translation="MREVKPISIDILNTFKQVDEDRLNELLADELKHLDRKIVVLDDD
                     PTGVQTVHDISVYTDWDKDSMEQGFNEKNSMFFILTNSRGFTVAQTTKAHKEISKNIV
                     DVSKKVNKDFIIISRSDSTMRGHYPVETNLLKSEVERLSGKLFQGEIIMPFFKEGGRF
                     TIDNVHYVKEGEILVPAGMTEFAKDKSFGYKSSDIGEWCEEKTNGKYKSSDMIYISSK
                     ELRELNIEAITEKLKKAEGFNKIIVNATDYVDVKVFTIAFIRAVNSGKEFMFRSAAAI
                     TKVLGGVSDKELLTKDELVSKGNTNGGIILVGSHVNKTTQQLEELKNCKYPIEFIEFN
                     QHLVLQENGLKNEVKRVIKVVEEKISSGKTVAVYTRRERFDLDTNDKDKQLMISVEIS
                     DAVTSIIGMLNVRPNFIIAKGGITSSDVGTKALRVKKATVMGQIKPGIPVWMTGKESK
                     FPNMPYIIFPGNVGEISTLRESVEILMG"
     gene            complement(20277..21131)
                     /locus_tag="KRL67_001196"
     CDS             complement(20277..21131)
                     /locus_tag="KRL67_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH1522093.1"
                     /translation="MSTFYIQFLYFVIYSFIGWCCETTYCSVLQKQFVNRGFLNGPFC
                     PIYGFGALAIVATLTPFVHNIPLLFLFSIIITSIMEYCTSFILEKIFNMTWWDYSKHK
                     FNIHGRVCLENSLMFGVLSLIVMLIVHPIVVDFINSISKNVLFIFAISIEIYFVLDLV
                     VTVHTILQLNGKLKQINLIIKELKDKKEYYKLITQETIENKLESLVENRLDTLEDRFE
                     ILKEKENYRYAKNRIDELKNKLNTLLSNHKLLHRRIIKAFPNISSNKHTDILNKIKEN
                     IKSIKKDK"
     gene            complement(21399..21722)
                     /locus_tag="KRL67_001197"
     CDS             complement(21399..21722)
                     /locus_tag="KRL67_001197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1540 domain-containing protein"
                     /protein_id="HBH1522094.1"
                     /translation="MERINCNVINCSHNNSHICYADKIFINGEKAHNDRHTCCSSFLD
                     ESIYSNLSSNVNNRNCEELVCTVKNCKYNKDGSLCTLDNILVAPETDRVNLYIETCCS
                     SFECK"
     gene            21925..22554
                     /locus_tag="KRL67_001198"
     CDS             21925..22554
                     /locus_tag="KRL67_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH1522095.1"
                     /translation="MFEKKKKELFPWKISALILVGVFVLSSGVYIGTRLKHNGVQTGE
                     VVSTSANMNEKSKEAFGLNKNCEVWLQSEGENETNYKRTLTMAGIVPESLLDKTKSEI
                     VSYFKKEYPTKKIKSMDKNEIILVESKYSDNKDVQAMDVTIKGKYTIENDNGNIALYK
                     YDDSGSKTLVEKTKIRVDSLPKTVQDEIKKGVVMDTEEEAYSRLEDFAS"
     gene            22867..23376
                     /gene="ruvC"
                     /locus_tag="KRL67_001199"
     CDS             22867..23376
                     /gene="ruvC"
                     /locus_tag="KRL67_001199"
                     /EC_number="3.1.22.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HBH1522096.1"
                     /translation="MIILGIDPGIAIVGYGIIEYKNSKFKVIDYGAVTTPAHMNISRR
                     LELVYKGIDTIVKNYNIDEVGMEELFFNKNVKTAITVAQARGVTMLACAHNGKPVYEY
                     TPLQVKQGVVGYGRADKAQVQQMVTSFLSLKKVPKPDDVADALAVAICHAHSNKLEKT
                     LKNIGGKYV"
     gene            23369..23971
                     /gene="ruvA"
                     /locus_tag="KRL67_001200"
     CDS             23369..23971
                     /gene="ruvA"
                     /locus_tag="KRL67_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HBH1522097.1"
                     /translation="MYSYIKGTVEEIYIDSIVVENNGIGYKINVSSNTIMNLQVGEAT
                     KIYTKLIVREDDMSLCGFVSREELKMFELLTSVSKIGPKVALSILSFASPAQLGAYIL
                     SEDIGKLSKAPGVGKKTAERIVLELKDKVDKNNIEFEPTLLSQKPTLISQDESVDALV
                     ALGYTLSESKEAVQKCKKDGMNTEAIIKKALTYIMSKSLK"
     gene            24062..25081
                     /gene="ruvB"
                     /locus_tag="KRL67_001201"
     CDS             24062..25081
                     /gene="ruvB"
                     /locus_tag="KRL67_001201"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HBH1522098.1"
                     /translation="MQGFEDENRIITSTMKMEDIDIENSLRPKTLEDYLGQEKSKEQL
                     SIFIEAAKSRNEQLDHVLLYGPPGLGKTTLASIIANEMGVNLRITSGPAIERAGDLAA
                     ILTNLNENDVLFIDEIHRINRSVEEVLYPAMEDFCLDIIIGKGPSARSIRLDLPKFTL
                     IGATTRAGMLTNPLRDRFGVICKLDYYTVDELSKIVLRSSSILDAEIQSNGALELAKR
                     SRGTPRIANRLLKRVRDFAQVRADGKITDKVAKDALELLGVDSLGLDFVDEKLLMTII
                     EKFRGGPVGLDTLAASIGEDRNTIEDVYEPYLLQLGFINRGPRGRVAMPLAYEHLKIP
                     YPNEK"
     gene            25399..26424
                     /gene="queA"
                     /locus_tag="KRL67_001202"
     CDS             25399..26424
                     /gene="queA"
                     /locus_tag="KRL67_001202"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HBH1522099.1"
                     /translation="MKTSDFKFDLPQELIAQVPIEDRASSRLMVLDKETGNIEHKVFR
                     DIIEYLNPGDCLVLNNTRVIPARLIGEKLETGGKIEFLLLKRTEEDTWQALVKPGKRA
                     KVGTKFSFGNGKLIGEVVDLSDEGSRIIKFHYDGIFEEILDELGNMPLPPYITARLDE
                     KERYQTVYSKHNGSAAAPTAGLHFTEELLNKIKEKGVDIAFVTLHVGLGTFRPVKVED
                     VLNHKMHSEYYMVSQEAADKINRAKANGNNVICVGTTSCRTVESACNEDGKMKETSGW
                     TEIFIYPGYKFKVLDKLITNFHLPESTLIMLVSAICGKDNVLNAYNEAVKERYRFFSF
                     GDAMIIK"
     gene            26436..26921
                     /locus_tag="KRL67_001203"
     CDS             26436..26921
                     /locus_tag="KRL67_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH1522100.1"
                     /translation="MHSLQKQKKNNPILKIAFIFERVLAVVVLIAVFLGTIDVLRLMW
                     GAYIVDFQNPVQYSQLNDFLAQILLLVIGVELVVMLSLHIPGAFIEALLYAIARKMLL
                     LPKNEGMIDVLIGVIAIAGLFAIKKFLVTKDKSALNIMSIHDEEAIKEQQNKEDEDKS
                     I"
     gene            27000..28121
                     /gene="tgt"
                     /locus_tag="KRL67_001204"
     CDS             27000..28121
                     /gene="tgt"
                     /locus_tag="KRL67_001204"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HBH1522101.1"
                     /translation="MYAVRYELIKTCKQSGARLGRLHTPHGIIETPIFMPVGTQATVK
                     SMTPEELKEIGSQIILSNTYHLYMRPGHELIKRAGGLHKFMNWDKPILTDSGGFQVFS
                     LGPLRKIKEEGVEFRSHLDGSKHFLTPEKAMEIQNALGSDIMMAFDECAPYPSDREYV
                     KNSLERTTRWLKRCKDAHNNTDKQALFGIIQGGMYKDLREQSAKEITSIDLPGYAIGG
                     LSVGEPKPLMYDVLEHTTPLMPKDKPRYLMGVGSPDDLVEGVIRGVDMFDCVLPTRIA
                     RNGTAMTSQGKVVVRNATYAEDFTPLDPECDCYACKNYSRAYIRHLIKANEILGARLI
                     TTHNLHFLLNLMKQIRQAIMEDRLLDFRNEFFAKYGYEI"
     gene            28255..28548
                     /gene="yajC"
                     /locus_tag="KRL67_001205"
     CDS             28255..28548
                     /gene="yajC"
                     /locus_tag="KRL67_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HBH1522102.1"
                     /translation="MPQQQIIMSIGLWVVVIAIFYFLMIRPQKKKDKQLKEMRSSLSV
                     GDKVITIGGIVANVAKVEDDVVILELGPNRTKVPFEKWAIGTVKSKKEEIEED"
     gene            28606..28968
                     /locus_tag="KRL67_001206"
     CDS             28606..28968
                     /locus_tag="KRL67_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04086 family membrane protein"
                     /protein_id="HBH1522103.1"
                     /translation="MGKSNYIFKGLGYAYIITLAVLLVYNLFLTFTDIGGDNITMVSS
                     FITTISAAIGGFYTSKHMKEKGLMYGLLVGLLYIVCIFLTVFLAQEKFVFEVGMIYKL
                     LLISAAGGIGGVLGVNFK"
     gene            29034..29177
                     /gene="scfA"
                     /locus_tag="KRL67_001207"
     CDS             29034..29177
                     /gene="scfA"
                     /locus_tag="KRL67_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="six-cysteine ranthipeptide SCIFF"
                     /protein_id="HBH1522104.1"
                     /translation="MENKHVKTLSQATLKQSAAKGGCGECQTSCQSACKTSCTVANQE
                     CER"
BASE COUNT        11140 a         3415 c         5009 g         9875 t
ORIGIN      
        1 aacagattat aattatagaa gggaaattta agatggattt taaaaattta cagtatgaaa
       61 gtttgtcata tattatttgt aacttacaaa aaaactttaa actttattgt gaaaaatgtt
      121 taaaaccata taaacttacg aatggtctat atttctattt aatttatatt aataaaaata
      181 ggaattgttc tttgaatgat gtttctacag aatttgaagt tgataaagct catacaacaa
      241 gaactatatc taggttagaa caagatggat acatagaaaa aatccaaaac cctaatgata
      301 gcagagcttt tcagttgaga gtaacagata agggtgagga agtattaggg gatataaaaa
      361 acatattttc aaagtgggat aaccgcataa aaaaagagtt ctcagataca gaatataaag
      421 aactagtaaa gaatttacat gtagtaaagg atataaaaac agctgtggag gaagaatagg
      481 atgtattgta atttattaaa accaattaaa attggaaatt tggaattaaa aaatagagta
      541 tcttttgcac ctacaagcat ggggcttaaa ttggaagaaa aaattaaaaa gttttctgat
      601 attgctaagt caggagtagc tttgattact ctaggagatg tatctattag acctagtttt
      661 cataagatag ctataagcct atcggatgaa gatggagtca tgaaatacaa gaaaattgta
      721 gatgaaatac acaattctgg agcaaaggta tcagcacaac tattttgttc agattatgat
      781 gttaatttaa ttaaagatac tatgaagatg ggaattactt cacatgatga gataaaaaaa
      841 ataatgaatg atggagtaaa agattacata acaaatatgc ccaaagaaga aattaaaaat
      901 attataaatt tatttaaagt aacagcattg aatgcaaaaa aagcaggttt tgacatgatt
      961 caaattcatg gtgataggct tgtaggaagt ttttcatcaa gtatatttaa taatagaaat
     1021 gatgaatatg gtggaacgtg tgagaataga agtagatttg caagcgaaat aattagtagt
     1081 atccgaaatg aagttaagga cattccaata gactataaat ttgcaataag acaagaaaac
     1141 cctcattatg ggaatgcagg agttttactt tcagaagttg agtattttgt taaaaaattt
     1201 gaatccttag gtataaacag ttttcatgta acacttgcaa atcattcaaa attagaagat
     1261 actataccaa ccaataatca tccttacttt aaagatgaag gctgtttttt atatttagct
     1321 gatgaagtaa agaaacacac aaatcttcca gtatgtgggg ttggaaaatt gtctagccca
     1381 gattttatag aaagtattat ttcaaataat agagtagata tgatttctat gtcaagacaa
     1441 ttactagcag atagtaattg gttacagaaa gtaaaagatg gaagagtaga tgagattaaa
     1501 aaatgctgtt attgtaataa gaaatgtgca gatgccttac agacacattc tcaatttgga
     1561 tgtatcttag attaaattgt gtttgtagaa ataaaaaatc tgttaatatt aatatatttc
     1621 ttgtaagtta gataattatt tagataaata ggaggatacg tcatgaaaag tttaatctta
     1681 tattcaagct taactggaaa tactaaaaaa attgcctgtt ctatatatga ggcaattcaa
     1741 gggaaaaaag aaattaaaaa tataagtgaa ttagttgatg atagtattga ttatgaaaat
     1801 tatgatatag tatttttagg ctattgggta gataagggaa tttgtgataa aaattcaaaa
     1861 caagtgcttg aaaatattca caataaaaag atagcactat ttggaacaat gggagcaagt
     1921 acaaaaggaa gttatggcgc tagtataata gaaaagatag aatcaattat tccaaaagat
     1981 aatgaaatat taggttcatt catatgtcaa ggtaagatag cagaaggatt gaaagtaaaa
     2041 tataaagaaa tgttaaaact ttctcctgat aacgaacata ttcgtcaaca attaaataac
     2101 catgaggaat ctcaaagtca tccagatgaa caagaaatat atgaagctaa tgtatttgca
     2161 aaaaatatga tgattaaagc tagtactatg taacagttaa acaaagagtt tatataggat
     2221 atagaatcaa taatccaata aaaagtatag caaatgattt gctatgcttt ttttatgaaa
     2281 ttaaaaaaat aataaaaata cttgactgta aaccttgttt atacattatt ttatagtaaa
     2341 taagatagtt ttaaatatag aaaaagagat attattgaaa ggagatatta ttgtatgaaa
     2401 atttataaaa gctacttagt ttgattttaa gacataataa acatacaatt ttatagagtg
     2461 aaagtattta agactatatt atagctataa attaatagaa tagagaggta gtgtttaaaa
     2521 tgacaattaa agaagttgaa gaaaaaacag gattgacacg ctctaatatt cgtttttatg
     2581 agaacgagaa tttaattaaa ccccttcgta atgagcgtaa tggctataga gaatattcag
     2641 atgaaaatgt agaggacatc aaaaaaattg catatttgcg tacattagga attactattg
     2701 ataatatccg taatattata ttccagaaag tatctttagt tgaagttgta gagaaacagg
     2761 agaaagttct cgaaactaaa atatcagact tagaaaatgc aaaaactatg tgtaataaaa
     2821 tgttagaatc tgatgagcta agttatttga atttgaaagt agagcaatac ataactgagt
     2881 taccagaata ctggaataaa aacaaacctg tttttaaact tgactctgta agttttttat
     2941 atatatgggg agggaaaatt acctggggaa ttattactat tgcttgtttg ttgattgcaa
     3001 taacagtatt tccaaagttg ccacctcaaa ttcctatcca atggaataat ggtgtagcta
     3061 attatgtaga taaaaaattt atttatgcct accctgtcat ttgtattgta ttcagaatac
     3121 ttttacgtcc atttatatgg agatggttac aaatacatgc tttttatagt gatactattg
     3181 cagattattt aacgaatttt atgtgttttg tagccttgtc tatagaaata ttctctatac
     3241 tttttattta caatattgtc agacatgtta cagtaattat ttttatagat atacttgtat
     3301 ttattgcttt actgataata ggtgtgtata aattatttaa aaaatataat taacttttaa
     3361 aaataagtaa taaatgatag cgtaaaaaat aaccactgca aattatggat tgtagtggct
     3421 attttattat ttgtatatga aattattttg tttttctaat aagctacgat atattatagt
     3481 ttatttcact gataaaaggt ttaggtattt taaagtaaaa agttgtatat tctaataaaa
     3541 tttaaacatt tttattcttt aaaattagag aaaatacatt gcttttatta aaaattaaat
     3601 attaagaatt taatttctgc atcttattta gaaaagataa gaaatcaact ttatatattt
     3661 tttttggcct tcctctttga ttatcgagtt ttgtatttac aatagttgca attcctgctt
     3721 tatataagat actcaatatt ctattagcac ttctaagagt tatgttcata tagtctgcta
     3781 atatttctgc tgatacatta tttgaatttc tatcattaat catacacatg attttagata
     3841 catttgtatt agatagaggt ataaaagaag caactttatt ggctattgaa ctatcttcaa
     3901 tattattaga atttttattt gaatataaag gaccgattgt atcattagta tcatttacta
     3961 taaaagtaca gtttccattc aaagaagcag atttaccatt agcttgacga gcattttgct
     4021 ctgcatgaat atttgtattt ccaatgcccc aacctatatt taagctatat ttaatttctt
     4081 ttaaatcttc tgaaataaaa aaatctgtat agttatttgt catattgttt aacatttctt
     4141 tagtagtgtg tatctctatt ttcatatcat tttttttgac ctgaactcca taaaatccat
     4201 gtcttgatag agaatttaat atagtgttat atatttgatt ttgtatctca tcatttttaa
     4261 atatcaaatc ttcatttatg tactctgaac tatttactgt aactatacaa gttgcagatt
     4321 tatttttgtc taagttttgt aatgttattt cattcaccag agatttaaat gtattcagga
     4381 ttgattctgt agatggaacc atgtaaatac aatcatatcc aagcttatta agttcgcaac
     4441 taatgatgta atttctagta aaagaaaggt taatcttatc atctcgcaat aagtctaaat
     4501 gtatttccaa taatttatca tttaattttt taaagtcata cacaggttga gggatattaa
     4561 aatctttaat tgtatatgga catttattag aatctattaa tgaatatata tcataataat
     4621 tgttgtcctc tcttaaaaaa tccataaatg attttgtaaa gtcaatatct ttttgactat
     4681 ttaatagttt aaataagtat ttataaaaat ctctttcatc aagctttaaa aagtttaatg
     4741 gtacttttat gtcttttata tttattataa gttcactata tggaactata ccactacata
     4801 caaatccatc aaaattatct acttgttcgt aatatatatt aggtaaattg ttaaaaggat
     4861 tttctccctt aagctcaata aatgtaattt cgcaaatatc atttaactct ttaagaacct
     4921 tcttaaactt gtctgacata aaataagatg ttgtcaaaaa accaatttta tatttcataa
     4981 aacctccttt aaaatttcga gagtattgta actaatcaat caatttataa cctcatttta
     5041 taatagtaga taaaatgcgt caatttattt tacaaaagga taattgaaag aaaaatcaga
     5101 aaatataaaa aagactgaaa aatatgttga ctggcaggaa tgattgtgct attttgtaat
     5161 taaggactag tcttttaaaa tttcctaaaa taatcttaaa ttttaggaaa gaattacatt
     5221 ttatttaaaa ataaaggaga ttattatgaa tacatattta aatgagattt ctaagtttgt
     5281 tgatgagaat agagaagaaa ttgtttcatt atggaaagag attgttaata tggaaagcta
     5341 tacacattgt aaagaaagcg taaataagtt ggctaagaga ttaaagtttg agtttgaaaa
     5401 agaaggttta gattgcgagt tagtagatgt tggagataat ggtagtacat tgataggtac
     5461 acttggttcc aatatagata aaaagccaat aattttttca ggtcacatgg atactgtctt
     5521 tgaaacaggt acatttggag agaatccatt taaaataata gaaggaaaag catatggccc
     5581 aggtgtttta gatatgaagg gtggtataat aatttcctta tatgttatta aagcattaaa
     5641 taaaatagga tacaaagaaa gaccaataaa aatagtattt tctggagatg aagagatagg
     5701 acataaggat tcaacaggtg cagatgttat attaagagaa gcaaaaggtg gtctttgtgc
     5761 ttttaatatg gaaactggat tagtagacaa tagtttgtgt gtaggtagaa aaggaagaat
     5821 aggatgcaat atacatgtaa aaggtgtaga aacacatgca ggtaatgatt ttgaaggtgg
     5881 aagaaatgct atagaagaaa tggctaataa gatactacgc atacaaaaat tgactaattt
     5941 agatgttgga actacagtaa gtgtaagtgt gattaagggt ggaagagtgg aaaattctat
     6001 tccagaagat tgtagtataa aagtagacct tagatttgaa aaagtagaag aaatggagaa
     6061 tgttaaagaa caagttagag agatatgtaa agaaacttac atagaaggaa cttctacaga
     6121 agttaatttt ataagtgaaa tgatgccatt tgaaactaca gaagatgtta tgagatttca
     6181 tacatttgta aatgaagtgt caaaagaaaa tggatttgga gaattaaatg ctaagagact
     6241 tggaggtagt tcagatgcta gttatttaac tattgcaaac gttcctgcaa tctgttcatt
     6301 tggtgtaagg ggggaatgga atcatacatc aagagaatat gcagtagtag attctatgtt
     6361 tgagagggtg aaattaattt ctacagttat tttaaactta gataaatttg aggtataaag
     6421 ggggatttta tatggatgta acagctggag ataataaggt aacatggaaa gggtatttat
     6481 ctttatttgt attgatagta ttattttcag gagtatttaa agatagccaa ggcttcctta
     6541 aagcattaga ttttgctaat ctgacaggtt catttggtac tattgcagat ggtgtagact
     6601 ttatgggtaa aggaggaacg ggagcaaaag atggtttctt atttgctctg actttgattc
     6661 caactgtctg ttttgctgta ggtcttatag atgtagttga atctatggga ggaatgaaag
     6721 cagcatcaaa gatatttaat ccattactta gacctctgct tggcatacct ggaatagctg
     6781 gaatagcatt tgtatcttca ttcactagtt cagatgtagc ctcaataatg acgaaagaat
     6841 tatttgaaag tggggaaata acagatgatg aaagaactat atttgtagca tatcaatatg
     6901 ccggttcagc tgttatactg aacacaataa atactcaagc tccactttta ccaatagcat
     6961 tattggcatt aggtccaata atccttatag agatattctg taaaatactt ggtgctaatc
     7021 tagttagatt aatcataggt gtaagaaata aaaagaataa ggctaaggag gctgtgtagt
     7081 atgagtcaac aaaatactgg aaatgcaatt gtaaatagta aacctaagaa aactgctgtt
     7141 gagatattta tggggggggc aaaaaaaggt ttttacatag gtgttgaaca gatattacca
     7201 gctatgataa tgggttatgt aatagtgcag tttttacaat taactggttt agttgatatt
     7261 ttaggaaata tttttggacc agtaatgggt atttttggac tacctgggga atctgttgta
     7321 gttttgatag cagcattctt tgctaaggca gcaggagcag ctacagccgc aaacttgttt
     7381 gcacaaggtc taatcactgc accacaagca accatattaa ttatgccatg tatgttaatg
     7441 ggtactctag ttggtcatta tgccagaata gtgcttgtag caaatgccag tactaagtat
     7501 agaatgttac tgttggcagt tcctatattt gatgcaatag tggggatgtt gatagtacga
     7561 gttttactta ctgttatggg attaatgtaa gtaacataaa attatagcct aattataaat
     7621 gatatataag aattaatata tacaagaatt gatgaatagt aattaatata tagtaattga
     7681 tatatagtaa taaaataata tagtaattaa tatgtatatt gtatatcaat taaaaaagaa
     7741 gagagtatct caaaatgaac tttttagttc atgagatgct ctttttgtat gaaatcaagt
     7801 atattttcat aatttcaaca atgaaagaga ggcttatcta tattttgaga cagcctcttc
     7861 ttttaaattt atatttctag aatttatgtt ttttatttat acaacattaa cattagttat
     7921 caacaataat tttaagaaaa atatactttc atgttaatat caaaataaat gtgtttttaa
     7981 aggatgattt gttttgaatt ttcacgaata tctacttcta tgactttttc tattgtatct
     8041 cctctaaaac ctaataaatt acttgtcaag tttgcagtag aacaagaatg attaggtata
     8101 attcttattc tatcaccaat ttttatatca gtttcaccat ttactttaat ttttccaact
     8161 tcctctgaaa gtgaatcaat agtaagttct ggatgtccac ataccctacc ataaccaaca
     8221 atactactat ttccatgtgc acctttatca agtccaaggc acttacttcc acaatcaatt
     8281 aaatagtatc cactgctatt ttttgagatt acagtgcaca caacagtaag agcacacata
     8341 tcttcagtag cacaacctaa acctatctgt atagcatcat agtatacata gtttccgggt
     8401 cttacagcag ttatagtttt agaagcagct tcagcttcaa ctgttggagt actaccagaa
     8461 gcgacaattt ctatattaaa tccattatca gttagagctt tgtatgcttt ctccatagaa
     8521 gaatcttctt gaacacaaac atctggaaca ccttctggag aactacaacc atacacttga
     8581 cctggatgtg aacttatacc aacaattttg atgttttcta gcttagaaat ttcagtagca
     8641 agttcaacca ctttttctgg ttcaacccca aaacggtgta aaccacaatc tacaataata
     8701 agtagcttgc actctatttt tgatttagaa aaagcttcac tataagcctt agcacattct
     8761 atattatctg tagcacaaat taaatttatt tttgatgcca tagtcacaat tctatttata
     8821 ttgacctctc ccatgtaagg ataagccaac ataatatctt caaaacctgc ttttatcaaa
     8881 gcttcacctt catcaagtgt tccaactaga aaaccactag cacctgcatt tttttgaagt
     8941 ctcgctatgt aagtactttt atgtgtctta agcataggcc aaagttgttt attattattt
     9001 gtacaaattt gttgaatttt atctatattc ttttctaact ggtctaaatc tagcaaaaaa
     9061 tttggagtag cgacttctga aattttcatg ttatttacca cctttcaaca aaagtaaatt
     9121 aactataagt attataatta ttatattagt aatatcggct ttataatatt aatagcttat
     9181 aattatatta taaaactagc gttaacagag agcaatattt atttgtaata attagaattt
     9241 tcaataagtt attgtggttt taaattattt atataatata tttttaattt tatgcaaagt
     9301 ttatttaaat tagtttgagg atttattaat agggtattat tgaatgagta aaaaagtgat
     9361 gtttgcatag aataatcaca ctataaatgt tggaggtagt aaacatgaga aaagtagtac
     9421 tttatattgc cacaagttta gatggttata tagctgagga gaatggcagt gtaagttttt
     9481 tggacgagat atcttcagaa tttaagaatg agcaggaaga tgattataat actttttacg
     9541 atacaattga tactatcatt atgggaaatt ccacatatgt acagattgca aatgaattaa
     9601 gcccaaatga atggtactat aagggtaagg aatgctatgt atactcaaat acaattaatg
     9661 ggacaactga gaatgtaaag tatactaatt tagaacctaa aaaattaatt gagcaaattg
     9721 gaaaagagaa ggagaaaaaa gatatttgga tagttggagg aggcgatata gtaaaattat
     9781 ttatgaaaga taacttaatt gatgattatt atatatatat attgccaata atattgggga
     9841 gagggatttc actatttaag tctggtatag ataagactaa tttgaatttt aagaaagcta
     9901 gtaatattgg agaacttgta aaattggaat acagtaaaaa acaaaaataa attacatgcc
     9961 aatttgttac aatgtcatga aatggagcat ttaaaagact tttggctata tgaattgaca
    10021 ttacttgtca aatatgttaa catataagcg acttgtcgga gggtgtatta aaaagaatat
    10081 gctggagaag agagtgcgtt aactcttttc tccttttttt ataaaattaa gcatttataa
    10141 atttagtatt attacatgat tacactatct caaaaattaa gaaaggagaa aaatatgaaa
    10201 ttaattttaa gttatttaaa gaattataag ttattaatag tattaaacgt actggctata
    10261 tttagttttg cattagtcga gttggggatt ccaactataa tagctaaaat aattgacaat
    10321 ggtattgcaa atcagaatat agcttacata aaacaaatgg gaatagtgat tgttattatt
    10381 tctattatag gggttgtagg tagtatattg ctgggttatt gttctgctaa ggtatcaaca
    10441 agtgtaacca gagatataag aaatgatata tttgagaaat cacaagaatt ttcacatact
    10501 gaatacaata aatttggaat ttcttcaatg attacaagaa caacgaatga tgcatttcaa
    10561 attcaacaat ttgttaatat actactaagg actgccttat taactccagt tatgtttatc
    10621 ataagtataa taatgacaat tagaacaagt gtggaacttt ctatagtatt ggctatatca
    10681 gtacctttta taattattgg agtagcaata atagcaaaag tatctcaggc aatatcttca
    10741 aaacaacaaa aaggactaga taaattaaac cttatatcga gagaaaattt aactggaata
    10801 agagtaatta gagcttttgg aaatgatgat tatgaaaccg agcgatttga aaaaacaaat
    10861 acatattatg ctaatgtttc taagaaatta tttaagctta tgtcaattac tcaaccagca
    10921 ttctttttac tattaaacat agctgttttg gctgtatttt ggatttcaag tgagaagata
    10981 aatattggag aacttcaagt tggacagttg gttgcatttt tggaatatct tttccatgcc
    11041 atgttttcaa ttatgctttt ttcaatggta tttattatgt atccaagagc agaggtatct
    11101 gcaaatagaa taaaagaact tttaaatgaa gaaccattga taaaaaatcc tgaaaatgga
    11161 attaaggata ctgaaaataa gggtatcata gaatttgata atgtaacttt tacctatcca
    11221 gatggagaag cttctgtact taaggatatt tcatttactg caaaaacagg tgaaacagtt
    11281 gcttttatag gaagtactgg tagtgggaaa agtactctaa ttaacttgat acctagattt
    11341 tacgatgtaa cagaaggtag cattaagata aatggagtag atataagaga atatgattta
    11401 aaagctttaa gaaaaaagat aggattcata cctcaaaaat cactgctttt tactggtagc
    11461 atagcaaata atattagatt tgggaaacat aaggcaggag agtcagaatt agaatattcc
    11521 gcaaaagttg cacaagcata tgagtttata tcaaaaaaac ctagaaagtt tgatgaattg
    11581 attagtgaag gtggagcaaa tgtatcagga ggtcaaaaac aaaggctttc tattgcaagg
    11641 gctattataa gaagacctga aatatacatt tttgatgata gtttttctgc acttgacttt
    11701 aaaactgatg caatacttcg tgctaaattg aagaaggaaa caaaagatgc aattgtattg
    11761 attgttgccc agagaattag ttctattatt gatgctgata agattatagt actcaatgaa
    11821 gggcaagttg tcggaatggg aactcataaa gaattattaa aaaactgtga aatatactat
    11881 gaaattgcaa catctcagtt aaaaaaggag gaattagaat aatgaaaaag tctaaaggtt
    11941 ttaagaaaac aattggtaga cttcttccat ttgtaaagaa atataaattt tcctttatta
    12001 tagctattat atgtataatt tcagcagcta ctatgaatgc acttgcacct aaaactgaag
    12061 gattgataat aacacaattg actaaagatg taattagtat tgcaaaagga gtaccagggg
    12121 cgtccgtaaa ttttgattat gtaacaaaaa tattggtgat tttagcatgt atatattttg
    12181 ccaatgcaat ttttacgtat acttcaagtt ttctacttac gaatgctatt caaaacacaa
    12241 tgagagattt aagaaatgaa gtagaaaata aaataagaag attgcctatt agttattttg
    12301 atagtaatag ttttggggat gtattaagtc gtatatcaaa tgatgtagat acaatatcaa
    12361 atgcattgca acagagtttt atgcaaattg taaactcaat tttagtcata attttggcac
    12421 tttcaatgat gtttacaatt aatatatata tggctcttat agctcttttt ataattccaa
    12481 ttagttattt tgtatctaaa tttgtagtaa aaaaatctca aagtagattt tcattgcaac
    12541 aaaatgctct tggaaaatta aatggaaaag tacaagaaat gtatactgga tttaatgaaa
    12601 taaaactata tggtaaagaa gaagattcca taaaagaatt taaaaaggta aatcaagaac
    12661 tttgtgaaaa tggatttaag gcacaattta tatcaagtat gatgaatcct atggtttcat
    12721 tagtaactta ctttggaatt gcagcagtgg cagttgtcgg ttctatatat gcagtaagtg
    12781 gagggataac agtaggaaat ttacaagcat ttgtgcgtta tatatggcag ataaatcagc
    12841 cactttcaca aatgactcag ttatccacag taatacaatc ttcttttgca gctatagaac
    12901 gtgtatttga aattttagac gaagaagagg aaatacctga cgttgaaaat ccagttaaga
    12961 tagaaaatgt aaaagggaat gttacatttg agcatgttaa ttttggatac ggagaaaatt
    13021 caactttaat agaagattta aatgcagaag taaaaagtgg tcagatggta gctattgttg
    13081 gacctaccgg agcaggtaaa acaacattaa taaacctatt gatgaggttt tatgatgtaa
    13141 agaaaggtgc tattaaaatt gatggagtag atataagaga tatgaaacgt aaagatttgc
    13201 gttcaatgtt tggtatggtg cttcaagata catggttatt taatggaacc atttttgaaa
    13261 atatagaata tggtagattt ggagctacta aagaagaaat tatacaagca gcaaaagtag
    13321 ccaatgttca tcactttata accactcttc cagatggata caatatgttc ttaaatgaag
    13381 aagcatctaa tatatcttta ggagaaaaac agcttttaac catagctaga gcttttatat
    13441 cagacccttc aatattgata cttgatgaag ctactagttc tgttgatact aggttggaac
    13501 ttatgcttca aaaagcaatg agaaatctta tgaatggtag aactagtttt gttatagcac
    13561 atagactttc tacaattcgt aatgctgact tgattttagt tatgaataat ggaagtatta
    13621 tagagcaggg gaatcatgat gaattaatgg aaaagggtgg attttatgag aagctttata
    13681 atagccaatt tgcagacaaa gaatcagaat agataacata aacttttata taaaattttg
    13741 tatcaaatta aaatattaat catataatta aaaagagtat cagtaagttg tttttttata
    13801 gatttactga tactctttga ttttaagacc aaaggtactt atgattggta cttataattc
    13861 acaactagtc ttgttacatc tgataaccag aattacagta cttttctttt ttaccttttt
    13921 gttctctaac atataacaat tcgcttaact tatcaatgta ataaccatca tcacttatgc
    13981 gaattaaact agtcagataa tatccatctt tgcttctagg aatcttttta tttattgcta
    14041 aatcaatagc tgtctttgct gcttcaaaat gctttaaatg tgtatgacct tcttcaaaac
    14101 tctttctagt attataaact acataacctt ttttaacttg aaaaactata aattctttct
    14161 tttcatatat tttattaaac attcaatcag cccttctcaa gatagtttta tagcataaca
    14221 ttagaagctt ctaaaaaatt ttacaaagaa cataaatatt ttataaatta taaatctaaa
    14281 actataattt ataaagataa ctttctgatt atttatatga taattcatat agattaaaag
    14341 taaattatct ttaggttaaa caaaagtaaa ataaaagact agttataata tttatatgaa
    14401 aaagtacaaa atatagtata tgaatataga aaatttaaat tatttattta cagtttttat
    14461 ttccataagt atatttacct tcagatacct tgctatcatg gaaatatatt ctttcacaac
    14521 ttttatctcc ccatagaata ttttttgtta ttttatggga cgtatctcct gtaaatactt
    14581 cagctacagt agtatctgta acatcacaat aaagagaagc gaatttttta aaccaaggat
    14641 tttcatcaaa tcttaaaagc caggaagctc cataagggca atgtaactct tcaaattcag
    14701 gaacccatcc tagaaatgga acatcagttg tgctaataat attgtctgca attaattcat
    14761 ctccattttc atgagctctt tctctcatag caatagctct ttcttcagac ctttcaaata
    14821 aaacttttct taatatttca ctagttttat cttcaccaaa ctcttcaaat aacactttgg
    14881 aaaaataaaa atataagtca ccaaaatgtt tgcatgccat acgaacttct tcaatagcat
    14941 aatctttgtc gatagtttca ttagttacaa catcttcgtt caaaaaaatc actccttaga
    15001 atagtattag ttacaatata attatagaat attttttata aattaggaaa ttaatttgag
    15061 atagatatat ttttaataaa tgaaagtata atactaaaat aacttattgt tacaaaaatc
    15121 ttctaaatta atgtatttag gagtttccat atgaatgaga gtagttctgt aaatatcttc
    15181 aatacatcca tttgcaatat tattaaaaat atctgtgtgc tcttttaatg ttgtttcaag
    15241 ccttccttca tgtgacaatg aatcgagagc taattttttt attctgtaca tataattgtt
    15301 aaacattttg tcaaattcag tattttgagc atatgaaact atagttgtgt gaaataatgt
    15361 atcgtattct gcaaatgagt ttaaattttt atcacctaat aaaatatctt tttgtttatc
    15421 taaaagatgt tttaatttac ttatagtttc tactgctcta ggttctttgt attgacttgt
    15481 aattaaaaaa gtacaaaatc cttctattgc agagcgtatt tggaatatct ctacaatttc
    15541 atttgctgta atttgatgga ttctaaatcc tttgcttggg attatatcta taaatccatc
    15601 ttgactaagt ctttgaagag catctcttat aggtgttcta gagataccta tttcactagc
    15661 aagttttgtt tcagaatata tttcattttc tatcattttt ccagatagta tcaattcttt
    15721 taagtagtca tatgcttgtt cttgtaatct tttaaatgta gccatactta ataatccccc
    15781 cctaaacaat ataaaaatac aaaaacttat ataattttaa gcatacaaca tagtgaaaat
    15841 aaggtcaatc ttttgcaatt taaattgcag ataagattaa aaaatataaa aaattattga
    15901 agtgtgaagc atgattaaca ttaaaggcag attaattaaa actaattgaa attagttaaa
    15961 atacaaaact gtaaaagtag tatattatat ttgaaaacaa tagtatgtat taaaacttat
    16021 ataggatgac taaatataat aaatgacttt aaaaattact atttattgtt aaaataaata
    16081 cttttatatt ttatattgac cggtatactg gtatgtagta tactctaaaa cataaaaaac
    16141 gttttcaaaa atgtgaagat atggagggtt ataaaatgaa attgggattt attggattag
    16201 gaataatggg aaagccaatg gcaaaaaatt tattaaaaga tggatgtaat cttttggtgt
    16261 atgacattaa taaatctgct gtggatgaac ttatatcatg tggagccaaa tacgcaagtg
    16321 ttttagaaat gggtcaagaa tgtgatattg tatttacaat ccttccaaat ggaactattg
    16381 tgcaggatat attatttggg atggatggat tggcaaaaac tttaaaagaa ggaagtatag
    16441 tagtcgatat gagttcagtt actcctacag aatcaatatt gtgtgcaaat aaattaaaag
    16501 atatgggctt agaatttata gactcaccag taagtggagg ggagccaaaa gcgattgatg
    16561 gtacacttgc ttttatggct ggtggtaagg aagaaattta taagaaagta gagccatttt
    16621 ttaatataat gggaagctca tctattttaa ttggagataa tggtagtgga agtgttacaa
    16681 aacttacaaa ccaagtaata gttaatttga cgatagcagc agtttctgag gcatttgtac
    16741 ttgcagcaaa agcaggtgca gacccagaaa aagtatataa ggcaataaga ggaggcctag
    16801 ctggtagtac aatattagat gcgaagatac caatgattat gaatcgtgat tttaaaccag
    16861 gtggaaaaat atctattaat ttgaaggata ttaagaatgt aatgcaaaca gcacataatt
    16921 tagatgtccc actacctatg acaagtcaat tattagaaat tatgcagaca ttaaaagtac
    16981 atggacattt agaagatgac catagtggaa tagcacagta ttttgaaaag ttggcaggtg
    17041 ttgaaataaa aaaacacaat aattaatatt caaatatatc aatataatta aatacatatt
    17101 attaggatta gattatataa atacagcgag gttaagttat cattgtaaaa gaaaaataag
    17161 actgataaat aataaagtag aatgataaag gggtaaaaat tatgattaca ggatatggac
    17221 ttttgatagc ttttgttata tctataggaa ttttacttgt atcgattatt aaatttaaag
    17281 taaatccgtt tttggcatta ctgataacat ctataattac aggctttatg gttagaatgc
    17341 ctataaatga aatctcaacc acaatatcga ctggatttgg aaatacatta gggagtatag
    17401 gtattgtaat tggacttgga ataatatttg gaaatattct ttcagaatca agagcaacag
    17461 aatctattgc aaaggggttg ttagctaaga ctggagaaaa aaacagtgct ttagcagtaa
    17521 ctacagcagg ttttttaatt tcaattccag tttttatgga tgcagctttc gttataatga
    17581 tgcctataat taagtatgtt tcgagagtaa ctaagaaatc tataatggtt tttgtatgtg
    17641 ccttaggagt tgggactata gtaggtcatg cacttgtaat acctacacca ggacctttgg
    17701 cagttgcagc aaatgtaaat gcaaatgtag gtagttttat actttattct ataattgttg
    17761 cttttccagc agctcttata ggtggatgga tttatggaaa acgttttgaa aaatatccag
    17821 cttatgctat tgatgagaat gatagagaaa aaaatttaga acagggaaaa ggtagcataa
    17881 aaattgaaga tgatgattct tctaaagtac caggttttgg aatttctatg ttttctttat
    17941 tgttcccaat acttcttata cttatatcaa atgtatttag tatgttctta gaaaaaggtt
    18001 ctacaatgag tggagtactt gcatttatag gagataaaaa catagcaata cttcttggaa
    18061 tattggtggc aataggattt ttaaagaaat acataaataa acctatggga gatgtagtta
    18121 ttgaagctgc tgattcagca gggctaatat tattgataac aggttcaggt ggtgcgtttg
    18181 gcagtgttat aaatgcaagt ggtataggta acttcttagt tgatactatg tctggtctta
    18241 gtatatcagt tgtagtgctt ggattcttat taagtgcctt gcttagaatt tctcaaggtt
    18301 ctgctactgt agccttagta acaacatcat caattttagg acctacaatt ttggcaactg
    18361 gaatgtcacc agttttagta gggcttgcta tatgtgctgg aggagttggt ttctcccttc
    18421 caaatgattc tggattctgg gtactttcaa gatttagtgg attatcagtt aaagatactt
    18481 taaactcttg gacaattgga ggaaccattg ctggagttgt agcatttata atggttttat
    18541 tgcttagtgt tataaatgga attataccac ttccaggtct ataaataaca aactattgtt
    18601 tgtatcatat aaattacatt tagactatac aaattacata aaggggtgtt ttaaatgaga
    18661 gaagtaaaac caattagtat agatatactt aatactttta aacaagtaga tgaggacaga
    18721 ttaaatgaac ttcttgcaga tgagcttaag catttagata gaaaaattgt tgtactagat
    18781 gatgacccaa caggtgtaca aacagttcat gatatttctg tttatactga ttgggataag
    18841 gatagtatgg aacaaggttt taacgagaaa aatagtatgt tttttatact tactaattca
    18901 aggggattta ctgttgctca aactacaaaa gcacataaag aaatttcaaa gaatattgtg
    18961 gatgtatcaa agaaagttaa taaggatttt ataatcataa gtcgtagtga ttctactatg
    19021 agaggacatt atcctgttga aactaattta ttaaaaagcg aagtagagag attatcgggg
    19081 aagttatttc agggtgaaat aataatgcca ttttttaaag aaggtggtag gtttactata
    19141 gataatgtac attatgttaa agaaggagaa atacttgtac cagcaggaat gactgaattt
    19201 gcaaaagata aatcttttgg atataaatca tctgatatag gtgagtggtg tgaagaaaag
    19261 acaaatggaa agtacaaatc tagtgatatg atatatattt cgtcaaaaga acttagagag
    19321 ctaaatattg aagctataac agaaaaactt aaaaaagctg aaggattcaa taaaattatt
    19381 gtaaatgcaa ccgattatgt agatgtaaag gtatttacaa ttgcgtttat acgagcagta
    19441 aatagtggta aggaatttat gtttagaagc gctgctgcca taacaaaagt acttggcgga
    19501 gtgagtgata aggaactgct tactaaggat gagttagtat caaaaggtaa tactaatgga
    19561 ggaattatac ttgttggttc acatgtgaat aagactaccc aacagttaga agaacttaaa
    19621 aattgtaaat atcctataga atttatagaa tttaatcaac atttagttct tcaagaaaat
    19681 ggtctaaaaa atgaggtaaa aagagttata aaagttgtag aggaaaaaat aagcagtggt
    19741 aaaactgttg ctgtttatac tagacgagag cgttttgact tggatacgaa tgataaagat
    19801 aaacagctta tgatttctgt agaaatatca gatgcagtta ccagtataat tggtatgctt
    19861 aatgtaagac caaattttat tatagctaag ggtggaatta cttctagtga tgttggtact
    19921 aaagcactaa gagtaaaaaa agctacagta atgggacaga taaaacctgg tataccagta
    19981 tggatgacag gcaaagaaag caaatttcct aatatgccat atataatttt ccctggaaat
    20041 gtgggagaga ttagtacact tagagaatca gttgagatat tgatggggta gtatattagt
    20101 gtattgaagt ttaaatgctt aaatttactg agttaaaatt aaaataaact aaaattataa
    20161 attaagatta aaaataatag tatattaatt agggtttaga gtattttaca aaaaatattc
    20221 taaaccttaa tcttatgtat tagtattttt attatttaac ttatgtgttt attttattac
    20281 ttatcttttt taattgattt aatattttct ttgattttat ttaaaatatc tgtatgctta
    20341 ttagagctta tgttggggaa tgctttgatt atacgtctat gaagtaattt gtggttagac
    20401 agcaaagtat taagtttatt ttttagctca tcaatacgat ttttagcata gcgatagttt
    20461 tctttttcct taagtatttc aaacctatct tccaaagtat ccagtctatt ttcaactaaa
    20521 gattcaagtt tgttttctat agtttcttgt gtaataagtt tgtagtattc tttcttatct
    20581 tttaattcct taattataag attgatttgt tttaacttac catttaattg taaaattgta
    20641 tggactgtaa ctactaaatc taaaacaaaa taaatttcta tagatatagc aaatataaat
    20701 agcacatttt tagagataga gttaataaaa tcaaccacaa taggatgtac tataagcatt
    20761 acaatcaatg ataatacacc aaacataagt gagttttcca gacaaactct tccgtgaata
    20821 ttgaatttat gtttagaata atcccaccaa gtcatattaa atattttttc taaaataaag
    20881 ctcgtacaat actccattat gctagttata attatactaa acaaaaataa caacggaata
    20941 ttgtgtacaa aaggagtcaa ggtagcaact attgctaatg ctccaaatcc atatataggg
    21001 caaaatggac catttaaaaa acctctattt acaaattgtt tttgtaaaac tgaacaatat
    21061 gtagtttcac aacaccagcc tataaagcta taaattacaa aataaagaaa ttgaatataa
    21121 aatgtactca taagcacctc atttctagtt agtataacac tatatactcg atattttatt
    21181 tttatgatta atttgctaaa ttcaatttac atattgatta taccaattta tagaaattta
    21241 tcaaatttaa ttacagttag aacattataa acatggttat aataatgaaa ttttatttaa
    21301 aataaataag ggtgtcaagt ataatcaaac taatagttgt agatactcta cactatgtta
    21361 gtttaattac atttgacacc cttatattta tactatgact atttacattc gaaactagag
    21421 caacatgttt caatataaag gtttactcta tcagtttcag gagcaaccaa tatattatca
    21481 agtgtacata agctaccatc tttgttgtat ttacaatttt ttacagtaca aactaattcc
    21541 tcacaatttc tattattaac gttgctagat aaattactat aaatagattc atctaaaaat
    21601 gaactacaac aagtatgtct gtcattgtga gctttttcac catttataaa aattttatct
    21661 gcataacata tatggctatt gttatgtgaa cagttaatta cattacagtt tattctttcc
    21721 aaagttatta cctcctaaac gattatttat tataaattat gtccatataa ttacaagaat
    21781 ataactatta agagaaaact accttttaga aaatatctat tcttagagta atttgaaata
    21841 taaatatatt ttcttctaaa atttacctaa attgtataaa aacttacagc ttggagaaaa
    21901 taatagaaaa aggaggcgta atcaatgttt gaaaagaaga aaaaagaatt atttccttgg
    21961 aaaatatctg ccttaatact agttggagta tttgtattaa gtagtggagt ttatataggg
    22021 acaagattaa aacacaatgg agttcaaact ggtgaagttg taagtactag tgcgaatatg
    22081 aatgaaaagt caaaagaagc ttttggatta aataaaaatt gtgaagtttg gcttcaaagt
    22141 gaaggcgaaa atgaaaccaa ctataagaga acacttacaa tggcaggaat agttccagag
    22201 tcattattag ataagacgaa gtcagaaata gtatcatatt ttaaaaaaga atatccaact
    22261 aaaaaaataa agagtatgga taaaaatgaa atcatattgg tagaatctaa atatagtgat
    22321 aataaagatg tacaagcaat ggatgttact ataaaaggta aatatactat tgaaaatgat
    22381 aatggaaata tagcactata taaatatgat gatagtggaa gtaaaacatt agtcgaaaaa
    22441 acaaaaataa gagtagattc acttcctaaa acagttcaag atgaaatcaa aaaaggtgta
    22501 gttatggata ctgaagaaga ggcctattca agattagaag actttgcaag ttaatagtaa
    22561 attaatacta tttcaagtta aatactaagt aagttaataa taaataattt aatattaaat
    22621 agattagtat taaaattgta gtcacattta attcctataa aaatgcaata atttatttgt
    22681 actaaatata aaagttatat tgaggtaaaa atcttgcttt gatataactt ttttatttga
    22741 ttttatattt tacatagagc attttatgat ttgttaatat tatttatgat ataatgattt
    22801 agaacataag tttttatgag aaatacattt tggtatttct tttattttta gggggaattt
    22861 taaaaaatga taatattggg gattgaccca ggtatagcta tagttggata tggtataatt
    22921 gaatacaaaa atagcaagtt taaggtaatc gattatggag cagttacaac acctgcccat
    22981 atgaatatat cgagaagatt ggaacttgta tataaaggaa ttgatacaat agtaaagaat
    23041 tacaatatag atgaagttgg aatggaagaa ttattcttta acaagaatgt aaaaacagct
    23101 ataacagttg cacaagctag aggtgttact atgcttgcat gtgctcataa tgggaagcct
    23161 gtatatgaat acactccact tcaagtaaaa caaggtgtag ttggatatgg gagagcagat
    23221 aaggcacaag ttcaacagat ggtaacttca tttttaagtc taaaaaaagt tccaaaacca
    23281 gatgatgttg cagatgcttt agctgtggct atttgccatg ctcattcaaa taaacttgaa
    23341 aaaactctaa agaatatagg tggtaagtat gtatagttat ataaaaggta cagtagaaga
    23401 aatttatata gacagtatag ttgttgaaaa taatgggata ggatataaaa taaatgtatc
    23461 atccaataca ataatgaatt tacaagtggg agaagccaca aaaatatata ctaaattaat
    23521 tgtaagagaa gatgatatga gtctttgtgg ttttgtaagt agggaagaac ttaagatgtt
    23581 tgaattacta acatctgtat ctaaaatagg acctaaggtg gcgctttcaa tactatcatt
    23641 tgcatcacca gcacagttag gagcatatat tttaagtgaa gacattggaa aattatccaa
    23701 agctcctggt gttggtaaaa agacagcaga aagaatcgtc ttagagctta aggataaggt
    23761 agacaaaaat aacatagagt ttgaaccaac gttattatca caaaaaccta ccttgatatc
    23821 acaagatgag tctgtagatg cacttgtagc tttaggctat actttgtcgg aatcaaaaga
    23881 ggcagttcaa aaatgtaaaa aagatggtat gaatacagaa gctattataa agaaagcact
    23941 tacatatata atgagtaaat ccttgaaata atatttagat tttaaaacaa tatttaagga
    24001 gtttatgtaa atttgaaaat atataaagta gattttcaaa agtacgagag gaggggtata
    24061 aatgcaaggt tttgaagatg aaaataggat aataacttct acaatgaaga tggaagatat
    24121 agatatagaa aacagtttaa gaccaaaaac tttagaagat tatttgggtc aagaaaaatc
    24181 caaagaacaa cttagtatat ttatagaagc agctaaatcg agaaatgagc agttagacca
    24241 tgtgctttta tatggaccac caggtcttgg aaaaactaca ctagcaagta taatagccaa
    24301 tgagatgggg gtaaatttaa gaattacgtc aggacccgca atagaaagag ctggagattt
    24361 ggcagccata cttacgaatt taaatgaaaa tgatgtttta tttatagatg agattcatag
    24421 aatcaataga agtgttgaag aagttttata tccagcaatg gaggattttt gcctagatat
    24481 aataataggg aaagggccat ctgctagaag cataagactt gaccttccta aatttacact
    24541 tattggagct actacaagag ctggtatgct tacaaatcca cttagagata gatttggtgt
    24601 tatttgtaaa ttggactatt atacagttga tgaactttca aaaatagtac ttaggtcttc
    24661 aagcatctta gatgcagaga tacagtctaa tggagcatta gaacttgcaa aacgttcaag
    24721 agggactcca aggatagcaa atagattact aaaaagagtt agagattttg cacaagtcag
    24781 agctgatgga aaaataactg ataaagtggc aaaagatgct ctggaacttt taggagtcga
    24841 tagtttgggt ctcgattttg tagatgagaa gcttcttatg actataatag aaaaatttag
    24901 aggaggacca gtgggtttag atacattggc agcttctatc ggagaagata gaaatacaat
    24961 agaagatgta tatgagccat atttgcttca attaggattt ataaatagag gtcctagagg
    25021 aagagtagcc atgccattag catatgaaca cttaaaaatt ccatatccta atgaaaaata
    25081 aaaatagatt atacgagtat ttataaaata aaagagtcat taaattaaaa tatttctaaa
    25141 atggctaaaa attcaaacat atccacaaaa tggtaattat atctgtagat actgtggata
    25201 aacacgataa aaattgtgtg tattgtatct atatattgtg gataagacat gtatatcgtg
    25261 gaaaaatatc tatatattgt ggatatggtg tctgttaaaa atagcaattt cttgatagat
    25321 tatggtaata ttgtggaaaa taagttgttt ttttgatatt attgtagctg gagaaatttt
    25381 aagagaggaa gtttaacatt gaaaacaagt gattttaaat ttgacttacc acaggaactt
    25441 atagctcaag ttcctataga agatagagct agctcaagat tgatggtatt agataaagag
    25501 acaggtaata ttgaacataa agtatttaga gatataatag aatatttaaa tccaggagat
    25561 tgcttagtat taaataatac tagagttata ccagctagat taataggaga gaagcttgaa
    25621 actggaggta aaatagagtt tttattatta aaaagaacag aggaagatac ttggcaggct
    25681 ctagtaaaac caggaaaaag agcaaaagta gggactaaat tttcttttgg aaatggaaag
    25741 ctaattggag aagttgtaga tttatcagat gaaggttcaa gaattataaa attccattat
    25801 gatggaattt ttgaagaaat cttggatgaa cttggaaata tgccactacc tccatatatc
    25861 actgctagac tagatgaaaa agaaagatac cagactgttt attcaaagca taatggttca
    25921 gcagcagcac ctacagcagg attacatttt acagaagaat tgttaaataa aataaaagaa
    25981 aaaggtgtag atatagcttt tgtaactcta catgttggtt taggcacttt tagaccagta
    26041 aaagtagaag atgttttaaa tcacaagatg cattcagaat attatatggt tagccaagaa
    26101 gcagctgaca aaataaatag agcgaaggca aatggaaaca atgtaatatg tgttggtact
    26161 actagttgta gaactgtaga atcagcttgt aatgaggatg gaaagatgaa agaaactagt
    26221 ggatggacag aaatatttat atatccaggt tataaattta aagttttaga taaacttatt
    26281 actaattttc acttaccaga gtctacacta ataatgttag taagtgctat ttgtggaaag
    26341 gataatgttt taaatgcata taatgaggca gtaaaagaaa gatatagatt cttcagtttt
    26401 ggagatgcta tgattataaa gtagggaggt aatggatgca tagtttacaa aaacaaaaaa
    26461 agaataaccc tatattaaag attgctttta tatttgaacg tgttttagca gtagtagttc
    26521 taatagcagt atttttagga actatagatg ttctaagact tatgtgggga gcatatatag
    26581 ttgattttca aaatccagta caatacagtc agcttaatga ttttttagca caaatattac
    26641 ttttagtaat aggtgttgaa cttgtagtaa tgctatctct tcatatacca ggagcattta
    26701 ttgaggcatt actttacgca atagctagaa aaatgttgct actgcctaaa aatgaaggta
    26761 tgatagatgt tttaatagga gttatagcta tagcaggact atttgctata aaaaagtttt
    26821 tagtaacaaa agacaagagt gctcttaata taatgagtat tcatgatgaa gaagcaatta
    26881 aggaacaaca gaacaaagaa gacgaagata aatctattta gacaataaat tatatgaata
    26941 gcttattaca aatatatctt aagaaataat gagaaataaa tatgattaag gagaacttta
    27001 tgtacgcagt aagatacgaa ttgataaaga cttgtaagca gagtggtgca aggttaggaa
    27061 gattacacac tccacatggg attattgaaa caccaatatt catgccagtt ggaactcaag
    27121 caactgtaaa atctatgaca ccagaagaat taaaagagat tggttcacaa ataatactta
    27181 gtaacacata ccatttatat atgagaccag gtcatgaact tataaaaaga gctggtggtc
    27241 ttcataagtt tatgaattgg gataaaccaa tacttacaga tagtggagga ttccaagtat
    27301 ttagcttagg gcctttaaga aagataaaag aagaaggtgt agaatttagg tcacacttag
    27361 atggttcaaa acattttcta actccagaaa aagctatgga aatacaaaat gcattgggtt
    27421 cagacataat gatggcgttt gatgagtgtg caccataccc atcagataga gaatatgtaa
    27481 aaaattcttt agaaagaaca acaagatggt taaaaagatg taaagatgcc cataataata
    27541 ccgacaaaca agctttattt ggtataatac aaggtgggat gtataaagac ttaagagaac
    27601 aatcagcaaa agaaataact agtattgatt taccaggata tgctattggt gggcttagtg
    27661 ttggagaacc aaaaccactt atgtatgacg tgctagagca tacaactcca cttatgccaa
    27721 aggataaacc aagataccta atgggagttg gtagtccaga tgacttagtg gaaggtgtta
    27781 taagaggagt agacatgttt gattgtgtcc tacctactcg tatcgctaga aatggaactg
    27841 caatgactag ccaaggtaaa gtagtagtta gaaatgcaac ttatgctgaa gactttactc
    27901 cacttgaccc tgaatgtgat tgttatgctt gtaaaaacta ttcaagagct tatataagac
    27961 accttataaa agcaaatgaa atactgggtg caagattaat aactactcat aatcttcact
    28021 ttttattaaa tttaatgaaa caaataagac aagcgataat ggaagataga ttacttgatt
    28081 ttagaaatga attttttgca aaatatggat atgaaatata ggttttgcac tattaattat
    28141 aatcatttta atatataata ttcaatagta ctcaaagata gagaaagtaa attgttatat
    28201 aaacatttat tatgatttac aaaagagtat aatttacaaa ggagtgaaaa attaatgcca
    28261 cagcaacaaa taattatgag tataggtcta tgggtagtag taatcgcaat cttttatttc
    28321 ctaatgatta gacctcaaaa gaaaaaagac aaacaattaa aagaaatgag aagtagccta
    28381 agtgtaggag acaaggtcat tacaataggt ggaatagtag ctaatgtagc taaagttgaa
    28441 gatgatgttg taatattaga attaggacca aatagaacta aagttccatt tgaaaaatgg
    28501 gctataggaa ctgttaaatc taaaaaagaa gaaatagaag aagactaaat tagtaattta
    28561 aaagcctttt ctcgaatata tattaatttg agaggaggtt tttttatggg aaaatctaat
    28621 tatattttta aaggtctagg atatgcatat ataataactt tggctgtttt attagtgtat
    28681 aacttatttt tgacatttac agatattggt ggagataata taactatggt ctcatcattt
    28741 ataactacta tttctgcagc tataggaggg ttttacactt caaaacacat gaaagaaaaa
    28801 ggacttatgt atgggctttt agtaggactt ttgtacatag tatgtatatt tttgactgtg
    28861 tttttagcac aagaaaagtt tgtatttgaa gtagggatga tttataagtt attgctaata
    28921 tcagcagcag gaggcattgg aggcgtattg ggtgtcaact ttaaatagag tttatattaa
    28981 agtagtttta tgatataatc aataggaaac aattagaaat ggaggcgttg aaaatggaaa
    29041 ataaacatgt aaaaacttta tctcaagcta ctttaaagca aagtgctgca aaaggtggat
    29101 gtggagagtg tcaaacttct tgtcaatcag cttgtaaaac ttcttgtaca gttgcaaatc
    29161 aagagtgtga aagatagaaa attattgatt tagttgtttg taagagggta tctcaaagtg
    29221 attcttttat cattatgagg tatcctcttt tattttcaat tattatataa aaatatgtat
    29281 tctatttatg taaaattaaa tttaatttct tatcgtttaa ttatgcaaga atatctaatc
    29341 atataagaac atttatttta aagtatctgc tcttatattt tattttttat caattacatg
    29401 tattagaata atttttaatt ttaaactcta atttatgat
//
LOCUS       DAEPXF010000030        29412 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain HB27
            SAMN13376389-rid12479423.denovo.032, whole genome shotgun sequence.
ACCESSION   DAEPXF010000030 DAEPXF010000000
VERSION     DAEPXF010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13376389
            Sequence Read Archive: SRR10518432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 29412)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29412)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001069624.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001069624.1
            Genome Coverage       :: 315x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/15/2021 01:54:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,165
            CDSs (total)                      :: 4,076
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 89
            rRNAs                             :: 10, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 10 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 19 of 69
            Pseudo Genes (incomplete)         :: 44 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 10 of 69
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29412
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13376389-rid12479423.denovo.032"
                     /strain="HB27"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="China: Hebei"
                     /collection_date="2017"
     gene            complement(<1..168)
                     /locus_tag="KRL67_001208"
     CDS             complement(<1..168)
                     /locus_tag="KRL67_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PucR family transcriptional regulator"
                     /protein_id="HBH1522105.1"
                     /translation="MEELKDFLERQLNKKINIYPYENQKLDASSHLVTFNNSIYVIDF
                     LDKNNLDNLKGN"
     gene            complement(198..1307)
                     /gene="ugpC"
                     /locus_tag="KRL67_001209"
     CDS             complement(198..1307)
                     /gene="ugpC"
                     /locus_tag="KRL67_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003242648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HBH1522106.1"
                     /translation="MSEVILKNISKLYSNGFNAVKNINIDIKDKEFIVLVGPSGCGKS
                     TTLRMIAGLEEISEGELYIGDKLVNDIEPKDRDIAMVFQNYALYPHLSVYENMAFALK
                     LRKLPKDEIDKKVKEAAKILDLLPLLNKKPKTLSGGQRQRVALGRAIVRNPKVFLMDE
                     PLSNLDAKLRTAMRTEITKLHQQLGTTFIYVTHDQVEAMTMADRIVVMKDGMVQQIAT
                     PQDVYDYPANIFVAGFIGAPQMNFIDVILIEENDEIYAQNEHIKLKLNKEKHYDLIKD
                     NYINNEVVIGIRPEDIHVEDTFIKSNPDTCFKSRIEIKELMGAETYAHLKLGENTITI
                     RFDSKNRINVGDDLTLSVDNSRIHIFDKETTLAIR"
     gene            complement(1460..2623)
                     /locus_tag="KRL67_001210"
     CDS             complement(1460..2623)
                     /locus_tag="KRL67_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014466331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBH1522107.1"
                     /translation="MKRAYKIEINPTTEQKSKIHQTIGVSRFIYNFYIAHNKEVYDSK
                     GEFISGMDFSKCLNNEYIPNNQDMKWIKDISFKATKQAIMNGDKAFKDFFKKTKGFPK
                     FKKKKNQDVKAYFPKNNKTDWTIERHRVKIPTLGWVRLKEFGYIPINSIVKSGTVSQK
                     SDRYYVSILVEEDDIQVSKCTNEGIGIDLGIKDFAICSNGSKFKNINKTSTVKKVEKK
                     LKREQRKLSRKYESLKVRNKNIKEGVATRQNIQKQIVKVQKIHQRLANIRTDYINKTV
                     SQVIEQKPSYITIEDLNVSGMMKNKHLSKAISSQKFFEFRTKLTAKCKQNNIELRVVD
                     RWYPSSKTCSQCGEVNKGLKLKDRVYKCECGLSIDRDLNASINLKNAKKYKIA"
     gene            complement(2595..3118)
                     /locus_tag="KRL67_001211"
                     /pseudo
     CDS             complement(2595..3118)
                     /locus_tag="KRL67_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015979000.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS607 family transposase"
     gene            3432..4169
                     /gene="cas6"
                     /locus_tag="KRL67_001212"
     CDS             3432..4169
                     /gene="cas6"
                     /locus_tag="KRL67_001212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endoribonuclease Cas6"
                     /protein_id="HBH1522108.1"
                     /translation="MSRISIFMESCNGKFPNENSMLSVSFIKNILNAENKDFAKNIFN
                     YGEERKNNKQIKDINTAIYIPNLIRSKNELLVDGDIIFNISFYNYSMFSKLYNGILKV
                     KELEYKGYRFKIKNIKIHKEHEIKENGVIFKTMSPIIVKNKEGKYLDVEDSNYIECLN
                     YIANLTLESIRGSGLRKPLEFIPLNFKKRVLKEKIRGFKVREYYYINAYAGTFFLKGN
                     MEDLNALYKMGIGYRRTENAGMVDILK"
     gene            4174..5640
                     /locus_tag="KRL67_001213"
     CDS             4174..5640
                     /locus_tag="KRL67_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH1522109.1"
                     /translation="MREIKAKFYRSGDIVRDIGIVYFYELLMDLKNELEKNDYKLKFT
                     CELNRNYLSLESAEYIDPKYISDYILENQLFKVFKNGKKETLEKIFSNIDNLTYVNFI
                     EELDKSSATEKQKEEIKKDFKNRRFPYVRNSGKYGLNTSLENMETNVKRIVNTVIKSN
                     IEIDKLDLTQYEEKDSVCNVCNINKTTKLDIDLRERKDSKYNFLFRGAEKSGFKRSGQ
                     VESNICFECEFFNLMCLLYINLKRPMVFAYTNDLRELAFLNHKIMLKRKMYSDKSFYK
                     KLLHEKISSIRLYRFDIDTNKGIILKFDSIIEYKELLKKIELIDIIDNYNFSREPGNT
                     KNLGKKMIDNGNLSNLKELLLDNISVLKQSTGTSREMDFVSSSYNIGLYIKLCWIVDG
                     GEEYKMKNYMESNLIYSRVGKDLFNKMTDESRRKNFSMKVIQLLKSNDRAQLFQTIMH
                     VLVSNGILIGEGFVEGIMQSSELELNTNVGIFIQEIMK"
     gene            5660..6673
                     /locus_tag="KRL67_001214"
     CDS             5660..6673
                     /locus_tag="KRL67_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DevR family CRISPR-associated autoregulator"
                     /protein_id="HBH1522110.1"
                     /translation="MNEKRVKGVTLTILTESPVPLSYDQGYGNYTPIKKEQYREKIHA
                     KTSIATITYDLRRKLHQEYGWNLSNIIFGKKGNIYPSIKKNVGCVDENGLETDVFGYL
                     IPLKDKGSISKASPLRIIPFRSLNPYRGSTQLITNRGFLSSEFGRKYYDEKEENEVLR
                     DENFPTTQALAIEETLSDYYVYTITLELDRIGVVEVEDGKLLLPEERKFMSKELREKA
                     VKDILDAITVFTRNIKHSSVLLKPLAVMGGAFDKVVPFFWDDVDYNADSGEINLEGVI
                     ETIESYSLKESNTILAINDRLKISNKKELNRFNLSKYPVKEIKNLADRLEIGEDNMWY
                     LKE"
     gene            6689..7486
                     /gene="cas5"
                     /locus_tag="KRL67_001215"
     CDS             6689..7486
                     /gene="cas5"
                     /locus_tag="KRL67_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated protein Cas5"
                     /protein_id="HBH1522111.1"
                     /translation="MKVLQCKLKQPTAHYRDPKVFQNEYISTLNLPSKTTIMGMITYL
                     CDRRLNSDIDIGIIGTHHHRELEFSRGENIDFWNEYTNMRKGKDKEKFLLKGNYYDYY
                     KEHKAQNSILNYEVLKEVELTIFISCKDDEELEFIRKKLESPCKYANLGRKEDFVIPS
                     EKGCFVKEVELKEVIPMNTRDAIKENIKLKNTYVRVDLRDKENVETIINQGVLIALPH
                     KYKDLEANRDDRVYEFCHYIYVDNDGIYPKNIKVNVCIDTKEVFTWL"
     gene            7513..9921
                     /locus_tag="KRL67_001216"
     CDS             7513..9921
                     /locus_tag="KRL67_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated helicase/endonuclease Cas3"
                     /protein_id="HBH1522112.1"
                     /translation="MKNIMQKEILAKSYNLRTNQQEQTLVEHIKDLFEVLESILELNL
                     YSDKDVEILKICCVLHDLGKINSIMQQKMEINNKISYSCSEEERKKLESDKKSLKKIA
                     RHNIFSGAFLKDILEKMNLSEEDKLYIYKSIMLHHGNYEDYMRLSTSKVQEEIYEYIE
                     KGILEKEEFNLKDIESYINDILNVDFKFDEDVLDYDYIDKLSESMFIDSDYNNGQIDD
                     SVLNYRKFKYILYKGMLNLIDHSASSRQKGIKFYNDFTDEEIDNMILSEIYKSQGNLE
                     KNNIKFNTIQKRLRELSGRNVLTVAFTGSGKTAADYRKTFKRKFFLVPNKISAESFYR
                     KNIFQNKNDLDTRSSNDYIGLLHGDINLYSENEDNGEHDFVLTLRDIDLSINFCKPCV
                     IATVDQLLLSMFKFPGYEKIFAAVKNASITVDEVHLLEPKMFLILIYFMQFACKYLDV
                     DFHLMTATLPKSYKEQMINKGIIFQEESNENVTDKGEIVFIESNKEEEICEGKDVKVS
                     FIKEKEIKSIVEDALENKQKILIIKNTIDSVNRTYEFLKENLSDKYGGVDIDVLHSRF
                     KFKDKKEKYSKILNGEGDIWISTQSVEISLDLDFNIIISDLATMDSLIQRMGRCNRNN
                     KYEYGNFYILPSEDKIYDNKLKNTTKSILNKIIKTNSIFTMGIRKIILADYYDNSVVK
                     KYFEENFISCDKEIKNIYGINKEIFDGLDLIFNFEPYKNIVDSKKEAAKIFRDVDVSY
                     KIILEEDFYKEDRELQQDSIQVSGFIFNRLYYYGLISKVEGYMVLKSSSKFEYNSTVG
                     LILK"
     gene            10362..11309
                     /gene="prmA"
                     /locus_tag="KRL67_001217"
     CDS             10362..11309
                     /gene="prmA"
                     /locus_tag="KRL67_001217"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HBH1522113.1"
                     /translation="MNNWIEVTIKTTTEAVEPITNILYEQGAGGAVIEDPKDFLFQKK
                     NELDWDYVEEEVFKKNEEDDVLIKTYVSEEKNVMEFVEIIKQKVLGLKDFGIDIGEGS
                     VSLDQVNEADWANAWKAYYKPTKVGQRVVVKPTWEDYAMQDGDLIIELDPGMAFGTGT
                     HETTSMCIRELEKYVNKDSKVFDIGCGSGILAIAAAKLGAKEVVAVDLDEVAVKVAKE
                     NVLENKVEKSVSVMHGNLTDVIKDKADVIVANIIADIIKILAKDVQNFMKEDAIFISS
                     GIILDKVEEVKESLIENGFEIVEVQKLGEWSAIVSKLKR"
     gene            11328..12086
                     /locus_tag="KRL67_001218"
     CDS             11328..12086
                     /locus_tag="KRL67_001218"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HBH1522114.1"
                     /translation="MDRFFVEKNNINLQDKTCTIEGEDVKHISKVLRCKLGEKLEICD
                     KNNNEYICEIMNIDKSIVNLEILEKVDINRESELKVRLYQGLPKAPKMEMILQKLTEV
                     GVEEIILVQTKRSVVKVDDKKEDKKFERWERIIYEAAKQSKRGKIPKLRGVLSFKEAL
                     EDMKKNNVNICPYENERTVSIKHALKKCDSNIDSVGIFIGPEGGFSEEEIEQIQKNNC
                     NVVSLGPRILRTETASVVASTIALYELSDLGGEK"
     gene            12088..13386
                     /gene="mtaB"
                     /locus_tag="KRL67_001219"
     CDS             12088..13386
                     /gene="mtaB"
                     /locus_tag="KRL67_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N(6)-L-threonylcarbamoyladenosine(37)-C(2))-
                     methylthiotransferase MtaB"
                     /protein_id="HBH1522115.1"
                     /translation="MKKVAFYTLGCKVNQYETEAMLELFEKDGYEQVNSEEYADVYVI
                     NTCTVTHMSDRKSRQYIRRVKKKNPDAIIAVVGCYSQVSPEEILDIEEVNLVMGTNDR
                     RKIVEEIKKINSSKKVSTVDDIMKVKAFEEIEISQTNGKTRAFMKIQDGCDRFCTYCI
                     IPYARGRVRSRDIDSIVDEVKKLANNGYKEVVLTGIHVASYGKDLKDRDIKLLDVIKQ
                     INQIEKIERIRLSSVEPILFTDEFVNEVLKMDKVCPHYHLSLQSGCDETLKRMNRRYT
                     TLEYKTIVDRLRSKMPDVAITTDVIVGFPGETNEEFKKTYEFLREIELSQMHIFKYSP
                     RKGTPAATMENQVDPQMKHFRSEQLLNLSKVNFNKFATKFIGRELDVLFEQNIEGNKY
                     EGLTSNYIRVVVESDKNIQGQILKVKINDVKDEYVEGILL"
     gene            13429..13779
                     /locus_tag="KRL67_001220"
     CDS             13429..13779
                     /locus_tag="KRL67_001220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine triad nucleotide-binding protein"
                     /protein_id="HBH1522116.1"
                     /translation="MDCIFCKIANGEIPSTKVYEDDRVLAFNDLNPVAPYHILVVPKK
                     HYDSLIDIPDKEMDIVSHIHVVINKIAKEKGFDQTGFRVINNCGSDGGQEVKHLHYHI
                     LAGKKLPNYEAGQN"
     gene            13942..14121
                     /locus_tag="KRL67_001221"
     CDS             13942..14121
                     /locus_tag="KRL67_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HBH1522117.1"
                     /translation="MSEVRVRENETLDSALRRFKRQCAMSGIMSEVRKREHYDKPSVK
                     RKKKAEAARRKNAKK"
     gene            14151..14594
                     /locus_tag="KRL67_001222"
     CDS             14151..14594
                     /locus_tag="KRL67_001222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HBH1522118.1"
                     /translation="MSLKQKLQEDLKSSMKNKDTVRKSVVTLIRASIKQYEVDNRVEL
                     DEDGIIDVIAKQLKQRRDALVEFEKAGREDLIKETEGEIEVLKEYLPQQLSEEELEEI
                     VKSTISEVGATSMKDMGKIMSVIQPKVKGRADGKLINKLVKQNLQ"
     gene            14705..15202
                     /locus_tag="KRL67_001223"
     CDS             14705..15202
                     /locus_tag="KRL67_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan-rich sensory protein"
                     /protein_id="HBH1522119.1"
                     /translation="MIISVKEIRNFLINIFIPLIIGYLSSILTMIISGTDISTYYLQL
                     TKPSFAPPAFIFPIVWTILYILMGISSYLILRKGYNLPKVRDAIFYYGLQLVLNFIWS
                     ILFFGFGLRFTALIDIIIMILVSIIMISKFSKIDKRSGYINLIYLMWLVYAGFLNYFI
                     WFINK"
     gene            15395..15958
                     /gene="raiA"
                     /locus_tag="KRL67_001224"
     CDS             15395..15958
                     /gene="raiA"
                     /locus_tag="KRL67_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HBH1522120.1"
                     /translation="MQIIVSGRQMKLTDGIKGYVDGKLSRLEKYLDPESEVKVTVSAK
                     KDRQKVEVTIIPINGQIIRAEDVEDDLYAAIDIVCDKLSRQVVKYKTKVKDKVQNNKS
                     IRFENLDFIDNSSEFDDYDYDDEEDENIVIERRKKFNVKPMSSEEAILQMELVGHNFY
                     MFRNQDNFEINIVYKRKAGGYGLIEQD"
     gene            16173..16409
                     /locus_tag="KRL67_001225"
     CDS             16173..16409
                     /locus_tag="KRL67_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YabP/YqfC family sporulation protein"
                     /protein_id="HBH1522121.1"
                     /translation="MLEISTDLQVNQPVITVTSNTFISIENYLSILEYEVDLIRIKTK
                     VKTIKISGDKLSLKYITDSEIGIKGIIYNVEYVD"
     gene            16422..17765
                     /gene="yqfD"
                     /locus_tag="KRL67_001226"
     CDS             16422..17765
                     /gene="yqfD"
                     /locus_tag="KRL67_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation protein YqfD"
                     /protein_id="HBH1522122.1"
                     /translation="MISFIRGYYVIVVEGVGLEQFLNHLIRNGISVYNVTRIKNTKME
                     FHIDRQDIKEFKNVYRGSKFDIKVKQKTGVPFIIKRVYKHKGMWICALVSLFLLMSTS
                     QFVTDVYIQSPEGIKKEALRNELYKVGVRPGVYKKSIDRKEVRDHMMSKFNDVAYLSI
                     NVKGTNIFVTVTKKAESLKSTDQSNYCNVIALKNGIIEKVIPRSGKSVIKSGDIVQKG
                     DVLLNGANTKSIPEVWASTFYESTKKASYVDTVNKKTGEKKNIYTLSFYDKEFTMRKN
                     IKYKDYVVENKEKKLSIGNYTFPIKIKTSTFYETKKVEVKKNKEELKKELSEKALKEL
                     EYIIPASARIIDVKHNFKVNKNMLEYLITVQTSENIAKIYPLSKSEAERFIKEESKPD
                     EGEEEVPSNPEKRPLNDIRNEFDEDNKDNKDQNNSDENNSNQNSNNSQNNNSNNN"
     gene            17704..18819
                     /locus_tag="KRL67_001227"
     CDS             17704..18819
                     /locus_tag="KRL67_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HBH1522123.1"
                     /translation="MKIIVIKIVIIVKIIIAITTNKIKLKILGGLIQLIIQKKFTIAD
                     GNFERELFGNFDENVKLIEKTLNIDVILREGNIILIGEEKNVDSALKLMNELHQTVSN
                     GKHLDKQSISYSLSLLLEGSEQKIKELEGTIVITQRGKAVQPKTLGQKEYIKLIENND
                     ITFGVGPAGTGKTYLAVAMAVKAFKRDEVSRIILTRPAVEAGESLGFLPGDLKDKVDP
                     YLRPLYDALFEMLGADKFNKYLERGTIEVAPLAFMRGRTLDNSFIILDEAQNTTSEQM
                     KMFLTRLGFGSKAVVTGDVTQTDLPQNKKSGLIQATEILKGVPGIGSIMLTDRDVVRH
                     ELVQRIIRAYEKHDKREEFKKEERKKAKMQEKTFKRK"
     gene            18876..19337
                     /gene="ybeY"
                     /locus_tag="KRL67_001228"
     CDS             18876..19337
                     /gene="ybeY"
                     /locus_tag="KRL67_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HBH1522124.1"
                     /translation="MDLILDDRQDKLEVSEELIEKIKDIIIECLDYEGYDDNYEVSLS
                     FVDNKEIHELNREYRGVDRVTDVLSFPLLSDDFEDVELEEESLGDIVVSLERALEQSI
                     EYNHSFEREVCFLICHSMFHLLGYDHDTDENTKEMREKEEHILNKLNITRE"
     gene            19343..20062
                     /locus_tag="KRL67_001229"
     CDS             19343..20062
                     /locus_tag="KRL67_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HBH1522125.1"
                     /translation="MKPEKTRQGIIKAFNAAIEGILYTFKFERNMKIHYLGSVAVLII
                     SLFFNFSKLEMIMLLMSICLVVVAEMFNTAIEKAVDLVTDEYHVLAKIAKDVAAGGVL
                     VAALNSVVVGYILFYDKLTDISGILIYKIRESELHITLICILLVLIAVVVVKALTSTG
                     TPLKGGMPSGHAALAFAIATAITLMTERVVASTLAYIMAVLVAQSRIEGKIHTFWETI
                     AGALLGVLIAILVFQLGMFYN"
     gene            20181..20582
                     /locus_tag="KRL67_001230"
     CDS             20181..20582
                     /locus_tag="KRL67_001230"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HBH1522126.1"
                     /translation="MDNIELLRLAEDARQHSYAPYSGFRVGAALLTKSGKVYTGCNIE
                     CASLGGTNCAERTAIFKAISEGDKDIHKIAIASDNSENNEQTYPCGICRQVIIEFGSD
                     IKIITGYTKGEVFEHNIKDLLPNYFSGNDLK"
     gene            20646..21539
                     /gene="era"
                     /locus_tag="KRL67_001231"
     CDS             20646..21539
                     /gene="era"
                     /locus_tag="KRL67_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HBH1522127.1"
                     /translation="MFKSGFVSIVGRPNVGKSTLMNNVVGEKIAIMSDKPQTTRNTIQ
                     AVYTDEEMQIVFLDTPGIHKPKNKLGEFMVKAATEAFKNVDLILFVVDDSKKIGPGDR
                     KIIEDLRSVKTPIILVVNKIDQLDQKDELFDIIKMYDREGIFKEIVPISALKGKNTDT
                     LIKVIQNYLEEGPKYFPDYMITDQPERVLIAELIREKVLHYLNDEIPHGVAVEIEKMK
                     ARKDKEIVDVSAVIYCERDSHKGIIIGKNGRKLKGIGKSARQDIELLLGSQINLQLWV
                     KVKENWRNLQNYINNFGYNDK"
     gene            21551..22930
                     /gene="mgtE"
                     /locus_tag="KRL67_001232"
     CDS             21551..22930
                     /gene="mgtE"
                     /locus_tag="KRL67_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HBH1522128.1"
                     /translation="MDRKLDASQDLLYEVKSLIDNNKVLELRELIEEYHIIDIFDIME
                     NLEEDMKIQLFEVLPLDMASSILEEGSVEFFISILSKLDVEHSKNILELMSLGDMADK
                     LSELEEEEREHIINLLNQENADYVKELLFYDEDSAGGTMTTGYISINKDMTALEAIDH
                     MREEAEEAETIYYIYVVDDEEKLVGVLSLRELIIARDANIVEDLMSENIISVYVDEDR
                     EEAVRLVSKYNLIAIPVVDRQEKLKGIITVDDIIDVMEEEATEDMYKFAGSSEHEREV
                     AEKENPTLREQIISALRGRLPWLIITLVGGLLASLILSNLDYIMNPVYAPLVFFIPVV
                     IGMGGNIGTQSSSVTVITLSNKDLNFSNVVREGIVGIITGLLCSIITGIVIYFVMRKL
                     DIVLIVSISLFINMVLGATIGAFMPVLLKKMDADPSTVSSPIISTALDITGIAVYFII
                     TTALLSKIV"
     gene            23003..23158
                     /locus_tag="KRL67_001233"
     CDS             23003..23158
                     /locus_tag="KRL67_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqzL family protein"
                     /protein_id="HBH1522129.1"
                     /translation="MNNISWEVFKKTGSIDAYLYFCDCKNIHEEIEEVREKKEDDDNP
                     EHPGDST"
     gene            23124..23897
                     /gene="recO"
                     /locus_tag="KRL67_001234"
     CDS             23124..23897
                     /gene="recO"
                     /locus_tag="KRL67_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HBH1522130.1"
                     /translation="MIILNTQGIVLKAIRYKESDIILTLFTRKLGKVSAIAKGAKKNK
                     SSLLSSSQLFSYSNFTLKKQGNMYKVTQSEIIKSFYNISYDIEAFSYATYITKLVENS
                     ILENQTNNRLFILLAQTLYLYTQDNTDNRFITAAFELKFLDYIGFKPIVNKCINCECK
                     ILQNSVFNIYEGGILCSKCSKLFDNNIKLDLTTISLMEYVLRNDILTCSKAKVSKYIT
                     HELENILDKYLKVYVDNINFKSLHVLQSVKNNKGVDNDE"
     gene            23890..24489
                     /locus_tag="KRL67_001235"
     CDS             23890..24489
                     /locus_tag="KRL67_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4342 domain-containing protein"
                     /protein_id="HBH1522131.1"
                     /translation="MSSNITIEMVDSVFERVPGATYAEAKEALVMCNGDVIEAVIYLE
                     NKKNTCNCKAKSAKETMEEVFGKDGENIKFQLKELLRKSSVVRIIVEKDGKTMMNIPL
                     TVGVVGLAFVPLATLVGLSAAVISKYRIKVQNEEDGEIVDLGELNEEKLHVLKNMIAN
                     AAKDVKDVVVDKKEDDKDVTADLMKEESEKNKEDEDENK"
     gene            24649..25527
                     /gene="glyQ"
                     /locus_tag="KRL67_001236"
     CDS             24649..25527
                     /gene="glyQ"
                     /locus_tag="KRL67_001236"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HBH1522132.1"
                     /translation="MNFQEMILALQKYWSKQGCIMMQPYDIEKGAGTMNPNTFLRSLG
                     PEPWQVCYVEPSRRPADGRYGENPNRLYQHHQFQVILKPSPDNIQELYLESLKEIGID
                     PSEHDIRFVEDNWEAATVGAWGLGWEVWLDGMEITQFTYFQQVGNIECELETGEITYG
                     LERLAMYIQEVDSVYDLKWNDKITYGEVFNKAEYENSMYAFELCDADMLFNLFDIYEK
                     EALRLMENGLVIPSYDYVLKCSHAFNTLDARGAIGVSQRASFIGRVRNMAKTVAETFV
                     KQREEMGFPLLKDGDK"
     gene            25527..27593
                     /locus_tag="KRL67_001237"
     CDS             25527..27593
                     /locus_tag="KRL67_001237"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HBH1522133.1"
                     /translation="MKNYLLFEIGVEEMPSRFVGSTLEQLKNNLSKLFDENRIAFDKI
                     NAYGTPRRLTLVVEGLSDRQSDLEEEIKGPAKKIAVDAENNLTKPALGFIKSKGLSES
                     DIYFKQVGKEEYIFATIKQDGKETSEVLKDILPETIKSVVFPKAMRWGGKNLKFARPI
                     RWIVTLLNDKVLEFDLEGIISSNVTKGHRFLGKSTIEVDSIEDYFEKLEKNYIVLDQN
                     KRKEMIRKQCIEVANSLGGEVEFDEDLLEEVTYLVEYPTAFYGEFDVDYIKLPKEVVI
                     TPMKQHQRYFPVLKEGKLLPNFIAVRNGDSYRIDNVKAGNEKVLEARLADALFFYRED
                     TKRNLESYIEKLKTVVFQVKLGTIYDKTLRLEKLSADILDSLGLVDEKADTIRAAKLS
                     KADLVTGMVGEFDELQGFMGKEYAKVGGENDVVSEAIFEHYLPRFAGDILPKTNAGVA
                     LSIADKLDSIAGFFAIGIQPTGSQDPYALRRQALGILSILMDRKVAVDIKVLIEHALD
                     NYSELDFNKEEVVEQIMNFFNERVKNLFKDLGIRYDVVDAVLSSDINDVSDMHMRALE
                     LNNWLEKDELVEMLTAFNRVSTLAQKAESDKIDESLLKEDAEIKLYNEFKQVKVKVNE
                     LLRDKKYGIALDEFASLRPVVDNLFDTVMVMDNDEAIKNNRLALLKQVYDTMLEICDL
                     SKIVYK"
     gene            complement(27758..29305)
                     /locus_tag="KRL67_001238"
     CDS             complement(27758..29305)
                     /locus_tag="KRL67_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite/sulfite reductase"
                     /protein_id="HBH1522134.1"
                     /translation="MDNFKKILLNELPNFKEYSSKFLNGEINKMQYKGFSGGYGVYAQ
                     RDKKSFMVRLRVSAGVLSQHQLNKIYEIAIKHNLDKIHLTTRQAIQLHDLSINSIVDI
                     MEEGIKNDIFTRGGGGNYPRNVGLSPLSGVDPSEAFDVTPYAVATDKYFIKNATTYHL
                     PRKLKVSYSNCYTDTAHCSIQDLGFVATLKNDEPYFRVFLGGGLGKQPKVALELDELI
                     KPKDALYCVEGMIKFFMDYGNYENKNRARVRYMVESLGEELFLEKFKEYYKLEKEKGS
                     LELNIEEIDYSKPGVKIDIQDSRLIPQKQDGLYTVYIHPVGGILLTKDLSTLLKELDN
                     IENPMIRLGMTEGLYILNLNGNEAKRMLEISKTISCNSQLEQSISCIGVPICQMGIQN
                     SQKMLHEIIDYFRLQNNEEILNKAPKLYISGCLNSCGVHQVGSIGLCGKKKNVDGIST
                     DAFELFVGGSFEIGKTRLGKSLGDFKASDIPEMLYKISDASSGNFYEWVNSNDDILNK
                     ITDKYKI"
BASE COUNT        11687 a         3103 c         5061 g         9561 t
ORIGIN      
        1 gttgcctttt aaattatcaa gattattttt atctagaaaa tcaataacat aaatagaatt
       61 attgaaagta actaaatggc tactagcatc taacttttgg ttttcatatg gatagatatt
      121 tattttttta ttaagttgtc tttctaaaaa atcctttaac tcttccataa agtctcctta
      181 aaaataagat tatattctta tcttatagct aaagtagttt ctttgtcaaa tatgtgtatt
      241 ctactgttat caactgaaag tgttaagtca tcaccaacat taattctgtt tttactgtca
      301 aatctaattg ttatagtatt ttcgcctagt tttaagtgag cataagtttc tgctcccata
      361 agttctttta tttcaattct tgatttaaaa caagtatctg gattagattt aataaaagta
      421 tcttctacat gaatatcttc tggtctgata cctataacaa cttcattatt aatataattg
      481 tcttttatta aatcatagtg tttttcttta ttcaatttaa gcttaatatg ttcattttgt
      541 gcataaattt catcattttc ttctattaat attacatcaa taaaattcat ctgtggtgct
      601 ccaataaatc ctgcaacaaa tatattagct ggataatcgt atacatcttg tggagttgca
      661 atctgttgaa ccattccatc tttcattact acgattctat cagccatagt catagcttca
      721 acttggtcgt gtgttacata gataaatgta gttccaagct gttggtgtag cttagttatc
      781 tctgttctca tagcagttct aagttttgca tctaagttgg atagaggttc atccattaag
      841 aataccttag gatttctaac tattgcacgt cctaaggcaa ccctttgtct ttgtcctcca
      901 gataatgttt taggtttttt atttaaaaga ggtagtaagt ctaatatttt agcagcttct
      961 ttaacttttt tgtctatttc atctttaggc aattttctaa gtttaagtgc aaatgccatg
     1021 ttttcgtaaa cagataagtg gggatataaa gcatagtttt gaaaaaccat agctatatct
     1081 ctatcttttg gttctatatc attgacaagt ttatctccaa tgtatagttc tccttcagag
     1141 atttcttcaa gaccagcaat cattctaagt gtagtcgatt taccacaacc agatggtcct
     1201 actaaaacga taaattcttt atctttaatg tctatgttta tattttttac tgcattaaat
     1261 ccatttgagt aaagttttga tatatttttt aaaattactt ctgacatagt attctgctac
     1321 tcaaaatatg gtcaaatata tccatatcga gatttttcct tcttcattga acttcgcctt
     1381 gcctaagcta ctacgatttg gtatagttct ccaaaggctt aaattccgaa gttagccctc
     1441 cgtacatata taagtgcttt taagctatct tatatttttt agcattttta agattaatac
     1501 ttgcatttaa atctctatca atagataatc cacattcaca tttgtacact ctatctttaa
     1561 gttttaaacc tttattaact tctccacatt ggctacaagt ctttgatgat ggataccatc
     1621 tatcaactac tctaagttct atattatttt gtttgcattt agcagttagt ttagttctaa
     1681 attcaaaaaa cttttgactt gatatagcct ttgataaatg cttatttttc atcattccac
     1741 taacatttaa atcttcaatg gttatatagc ttggtttttg ctctattacc tgggatactg
     1801 ttttattaat ataatcagtt cttatattag ctaatctttg atgaatcttt tgtactttga
     1861 ctatctgttt ttggatattt tgacgagtag ctactccttc ttttatattt ttatttctta
     1921 cttttaaact ctcatatttt cttgaaagtt ttctttgttc tctttttaat ttcttttcaa
     1981 cttttttgac agttgaggtc ttgtttatat ttttaaattt actcccattt gaacatattg
     2041 caaaatcttt aattcctaag tcaataccta ttccttcatt agtacattta gatacttgaa
     2101 tatcatcttc ctcaactagt atagatacat agtatctatc agatttttga cttactgtac
     2161 cacttttaac tattgaattt attggtatat atccaaattc ttttagtctt acccaaccaa
     2221 gagtaggtat tttgacccta tgcctttcaa tagtccaatc agttttgtta ttcttcggaa
     2281 aatacgcttt aacatcttga tttttctttt ttttgaactt aggaaaacct ttagtttttt
     2341 taaagaaatc tttaaaggct ttatctccat tcataatagc ttgttttgta gccttaaaag
     2401 atatatcttt aatccatttc atatcttgat tattaggaat atattcatta tttaagcatt
     2461 tagaaaaatc cataccgctt ataaattcac ctttactatc ataaacttct ttattgtgag
     2521 caatatagaa gttatatata aatctactaa caccaatcgt ttgatgtatt ttagattttt
     2581 gttcagtagt aggattaatt tctattttgt atgctctttt caacttcttc atcctctcta
     2641 atctttttct tgtattttct taatccatat atgcgacaac taaatatttg tcatatccaa
     2701 atcgaatgaa tctatcttta tgagttacaa taatcgtatt tacctcatta gtcatacaac
     2761 tatcaattag tttattccac tttttacgat tataatttag tccacttcca taatcttcaa
     2821 taacttcatc tactattatt cctttagcat ttgcatattg tttaagaaac tccacttgat
     2881 ttttcaaatc atctttttga ttagaaattg aaactcttgt ataaataaca ttttttctat
     2941 ttacttgttc tttatgtatt cctttatatt ctaaatattg ttcataagtg taatatcttc
     3001 tatctgttgg agttctaaaa gcttttaatt tcccttcaat atcccaacgt tgcagagttc
     3061 tggctgaaat attaattaac tctgcaaact cttttggttt acagttattg tttttcatat
     3121 aattatctct tgactataaa cagctatatt tgtttatgtt tttattaact aaagataagc
     3181 tcctgaatgt ttaaatttta attatatagt atcaaatttt tttattatat taaaggtgta
     3241 aaatgactaa atatatttgt ttaatatgta tagcaggttt ttagaaaaga acaaaacaaa
     3301 cagtacaata tatttaaaaa aattaaaaaa caaaattaaa tttattatca aaaaatcatt
     3361 ttatgatatg ttttattttt atattacaaa tagagaataa tgaattatac tagaaagtag
     3421 gaggtgaaaa tatgagcaga atttcaattt ttatggaatc atgtaatggc aaatttccaa
     3481 atgaaaattc aatgttgtca gtttccttta taaaaaatat tttaaatgca gaaaacaaag
     3541 attttgctaa aaacatattt aactatgggg aagaaagaaa aaacaataag caaataaagg
     3601 atataaatac agccatttac ataccaaatc ttataagaag caaaaatgag ttacttgttg
     3661 atggagatat aatattcaat ataagttttt ataattattc tatgttcagt aaattataca
     3721 atggaatttt aaaagtaaag gagctagaat ataaaggtta tagatttaaa ataaaaaata
     3781 taaaaataca taaagaacat gagataaaag aaaatggtgt tatatttaaa actatgtctc
     3841 ctataatagt aaagaataaa gaaggtaaat atttagatgt agaagattca aattatatag
     3901 aatgtttaaa ctatatagct aatctaactc tggagagtat aagaggtagt gggcttagaa
     3961 agccattgga atttatacct cttaatttca aaaaaagagt gttaaaagaa aagattcgtg
     4021 gatttaaagt gagagaatat tactatataa atgcttatgc aggaacattt tttttaaaag
     4081 gaaatatgga agatttaaat gcactgtata aaatgggaat tggatataga aggacggaaa
     4141 atgcaggaat ggttgatata ttaaagtagc gaggtgagag aaataaaagc taaattttat
     4201 agatcaggtg atattgtaag agatattggc attgtttact tttatgaatt actaatggat
     4261 ttaaaaaacg aactagaaaa aaatgattat aagctaaaat ttacttgtga gttaaataga
     4321 aattatttat ctttagaatc tgcagaatat atagacccta agtatatatc tgattatata
     4381 ttagaaaatc aactttttaa ggtatttaaa aatgggaaaa aggaaacctt agaaaaaata
     4441 tttagtaata tagataattt aacgtatgta aattttattg aagagttaga taaatcttct
     4501 gcaactgaga aacaaaagga agagattaaa aaagatttta aaaacagaag atttccatat
     4561 gttagaaata gtggtaagta tggattaaac acgagtctag aaaatatgga aacaaatgta
     4621 aaaaggatag taaatactgt aattaaatca aatatagaga tagataaact tgatttgaca
     4681 caatatgaag aaaaagatag tgtatgtaat gtttgtaata taaataaaac aacaaaacta
     4741 gatattgatt taagagaacg aaaggactct aagtataact ttttatttag aggtgcagaa
     4801 aaatcaggtt ttaaaagaag tggacaagtt gaaagtaata tttgttttga gtgtgaattt
     4861 ttcaacttaa tgtgtctttt atatataaat ttaaaaagac caatggtttt tgcatatacg
     4921 aatgatttaa gagaattagc atttttaaac cataagataa tgcttaaaag aaaaatgtat
     4981 tcagataaat cattttataa aaaacttttg catgaaaaaa taagttcaat tagactatat
     5041 agatttgata ttgatacgaa taaaggtata attctaaagt ttgattctat tatagagtat
     5101 aaagaattac taaaaaaaat tgaacttata gatattatag ataattacaa tttctcaaga
     5161 gagccaggga acactaaaaa cttaggtaaa aaaatgattg ataatgggaa tttatcaaat
     5221 ttaaaagaat tattacttga taatataagt gttttaaagc aatcaactgg aacttctcga
     5281 gaaatggatt ttgtatctag ctcatataat atcggtttat atataaaatt gtgttggatt
     5341 gtagatggag gggaggagta taaaatgaaa aactatatgg aaagtaattt aatatattca
     5401 agagtaggta aagacttatt taataaaatg acagatgaat ccagaagaaa aaatttttct
     5461 atgaaagtga ttcaactact taagtctaat gatagagctc agttatttca aactataatg
     5521 catgtgttag tttctaatgg aattttaata ggagaaggct ttgtagaagg gataatgcaa
     5581 agttctgaat tagagttaaa tacaaatgta ggtatattta ttcaagaaat catgaaataa
     5641 aaaaggggga aaatttaaaa tgaatgaaaa aagagtaaaa ggagtaacat taactatact
     5701 gacagaaagc ccagttccac tttcatatga ccaagggtat ggaaattata caccaataaa
     5761 aaaagaacaa tatagagaaa aaatacatgc aaaaactagt atagctacaa taacatatga
     5821 tttaagaaga aagcttcatc aagaatatgg ttggaattta agcaatatta tttttggaaa
     5881 aaaaggaaat atatatccat ctattaagaa aaatgtagga tgtgtagatg aaaatggatt
     5941 agagactgat gtatttggtt atttgatacc acttaaagat aaaggttcta taagtaaagc
     6001 ttctccactt agaataatac cttttaggtc attaaatcca tatagaggtt caacacagtt
     6061 gataacaaat agagggtttt taagtagcga atttggtaga aaatattatg atgagaagga
     6121 agaaaatgaa gtacttagag atgaaaactt tccaacaaca caagcattgg caatagaaga
     6181 gactttatca gattactacg tttatacaat aacattggag cttgatagaa taggagtagt
     6241 agaagtagaa gatggaaagt tacttttacc agaagaaaga aaatttatga gtaaggagtt
     6301 aagagaaaaa gcagtaaaag atatattaga tgcaattact gtgtttacaa gaaatataaa
     6361 acattcatca gtattattaa aaccacttgc agtaatgggt ggagcttttg ataaagttgt
     6421 accgtttttt tgggatgatg tagattataa tgctgatagt ggtgagataa atttagaggg
     6481 agttatagaa actatagaaa gttatagcct taaagaaagt aatacaatac ttgctatcaa
     6541 tgacagatta aaaatttcta ataaaaagga attaaatagg tttaatttaa gtaaataccc
     6601 tgtaaaagag attaaaaatc tagcagatag attggaaatt ggagaagata atatgtggta
     6661 tttaaaagag taggaggttt tttgaattat gaaagtatta caatgtaaac ttaagcaacc
     6721 aactgctcac tatagagacc caaaagtatt tcaaaatgaa tatataagta ctttaaattt
     6781 accatctaag accacaataa tgggaatgat aacgtattta tgtgatagaa gattaaactc
     6841 tgatatagat ataggaataa ttggaacaca tcatcataga gaactagaat tttcaagagg
     6901 agagaacatt gatttttgga atgaatatac aaatatgaga aaaggcaaag ataaagaaaa
     6961 gtttttgctt aaaggaaact actatgatta ctataaagaa cataaagctc aaaatagcat
     7021 attaaactat gaagttctta aagaagtaga actgactata tttatttctt gcaaggatga
     7081 tgaagaactt gaatttatta gaaaaaagct tgaaagtcca tgtaaatatg caaatttagg
     7141 aagaaaagaa gattttgtaa taccaagtga aaaagggtgt tttgtgaaag aagtcgagtt
     7201 aaaagaggtt atacctatga atactcgtga tgctataaaa gaaaatataa aattaaagaa
     7261 tacatatgta agagttgact taagagataa agagaatgtc gagactataa taaatcaagg
     7321 tgtattgatt gcattgcctc ataaatataa agatttagaa gcaaatagag atgatagagt
     7381 ttatgagttt tgccattata tatatgttga taatgatggt atatatccta aaaatattaa
     7441 ggtaaatgta tgtattgata caaaagaagt ttttacatgg ctttaaatta ttttgaaaga
     7501 taggtgttta tcatgaaaaa tatcatgcaa aaagaaatat tagctaagtc atacaattta
     7561 agaactaatc aacaagagca aacattagtt gaacatatta aggatttatt tgaagtatta
     7621 gaatctatat tagaactaaa tctatattca gataaagatg tagagatact gaaaatatgt
     7681 tgtgttttgc acgatttagg aaaaattaat tcaattatgc aacaaaaaat ggagattaat
     7741 aataaaatat cgtatagttg ttcagaagaa gagaggaaaa agttagagtc tgacaaaaaa
     7801 tctttaaaaa agatagcaag acacaatata ttttctggag catttttaaa agatatacta
     7861 gaaaaaatga acttgtcaga agaagataag ttatatatat ataaaagtat aatgttacat
     7921 catggaaact atgaagatta tatgaggtta agtactagta aagttcaaga agaaatttat
     7981 gagtatattg aaaaaggcat tttagaaaaa gaagagttta atctgaaaga tatagaaagt
     8041 tatataaatg acattttaaa tgtagatttt aaatttgatg aagatgtatt ggattatgat
     8101 tacatagata aattaagtga aagtatgttt atagacagtg attataacaa tggtcaaata
     8161 gatgattcag ttttaaatta tagaaagttt aagtatattt tatataaagg gatgctgaac
     8221 ttaatagacc atagtgcatc aagtagacag aaaggtataa aattttataa cgattttact
     8281 gatgaagaaa ttgataatat gattttaagt gagatatata agagtcaggg taatttagaa
     8341 aaaaataata taaagttcaa tactatacaa aaaaggttga gagaactttc tggaagaaat
     8401 gttctgactg ttgcatttac aggttcagga aaaacagctg cagattatag aaagactttt
     8461 aaaagaaaat tctttttagt accaaataaa atatcagcag aaagttttta tagaaaaaat
     8521 atttttcaaa ataaaaatga tttagataca agaagtagta atgattacat tggattactt
     8581 catggagata taaacttata ttcagagaat gaagataatg gagaacatga ttttgtattg
     8641 acattaaggg atatagattt gagtataaac ttttgtaaac cttgtgtaat tgcaacagtt
     8701 gaccagttat tacttagtat gtttaaattt ccgggatatg aaaaaatatt tgcagccgta
     8761 aaaaatgcat ctataactgt tgatgaagta catttattgg aaccaaagat gtttttgatt
     8821 ttgatatatt ttatgcagtt tgcatgcaag tacttagatg ttgattttca cttaatgaca
     8881 gctacacttc caaaatcata taaagagcaa atgataaaca agggaattat ttttcaagaa
     8941 gaaagtaatg aaaatgttac tgataaagga gaaatagttt ttattgagag caacaaggaa
     9001 gaagagattt gtgaaggtaa agatgtaaag gtttctttta taaaggaaaa agaaatcaag
     9061 tcaatagttg aagatgcttt agaaaataaa caaaaaattc ttataataaa gaatactatt
     9121 gattcagtca atagaacata tgagttttta aaagaaaatt tatctgataa gtatggtggt
     9181 gtagatatag atgttttaca tagtagattt aagttcaaag ataaaaaaga gaaatatagt
     9241 aaaatattaa atggagaggg tgatatttgg atatcaactc aatcagtaga aatatccttg
     9301 gatttggatt tcaatataat aataagtgat ttagcaacta tggatagttt gatacaaaga
     9361 atggggagat gcaatagaaa taataagtat gaatatggaa acttttatat tctacctagt
     9421 gaagataaaa tttatgacaa caaacttaag aacaccacta agagtatctt aaataaaata
     9481 ataaaaacta actctatttt tacaatggga attagaaaaa ttattttggc tgattactat
     9541 gataatagtg tagtcaaaaa atattttgaa gagaatttta taagttgtga taaagaaatt
     9601 aagaatattt atggtataaa taaagaaatt tttgatggat tggatttaat ttttaatttt
     9661 gaaccttaca aaaatattgt tgatagtaag aaggaagcag ctaaaatttt tagagatgtt
     9721 gatgttagtt acaaaattat tctggaagaa gatttttata aggaagatag ggagttacaa
     9781 caagattcga ttcaagtatc agggtttata tttaatagat tatattatta tgggttaata
     9841 agcaaagtag aaggatacat ggtactaaaa tctagtagta agtttgagta taatagtact
     9901 gtaggcttaa ttttgaagtg agcatatgtt taaatataat atacctttag acattgaaaa
     9961 tacaaaaagt actactgtaa gctcactgta aaaactattt ttttaaaaga aaaataagtt
    10021 gtgagataga gtgtttacaa gtgtatatag ataataaatt tgaggtttaa gattaactaa
    10081 gtgggtttca ggtacatcac gaaaataatt ataagttgga gattttgtat gtttaagatt
    10141 aactaagtgg atttcaaata ttgatatagt agatataata gaagttttat tattaggttt
    10201 aaaattaact aatcggattt ttaaatattc tgactattat ataaggatat tattagcttg
    10261 aatttaaaac taataatata aaacaacaaa atattatatt tatgatataa taaaagttat
    10321 ggaaaaacag gaatattact atagagaaga gaggtaaact tatgaataat tggatagaag
    10381 taactataaa aacaacaaca gaagcagttg aacctataac gaatatacta tatgaacaag
    10441 gagctggagg agctgtaata gaagacccaa aagacttttt attccaaaag aaaaatgaac
    10501 tagattggga ttatgttgaa gaagaagtat ttaaaaagaa tgaagaagat gatgttctta
    10561 taaaaactta tgtttctgaa gaaaaaaatg tgatggaatt tgtcgaaata ataaagcaaa
    10621 aagttttagg attaaaagat tttggtatag atataggtga aggaagtgta tcattggacc
    10681 aagtaaatga agctgattgg gcaaatgcat ggaaagcata ctacaaacca acaaaagtag
    10741 gacaaagggt agtagtaaaa cctacatggg aagattatgc tatgcaagat ggggatttaa
    10801 tcatagagct tgaccctgga atggcatttg gtacaggtac acatgagact actagtatgt
    10861 gcattagaga attagaaaag tacgtgaata aagattctaa agtatttgat attggctgtg
    10921 gtagtggaat ccttgcaata gcagcagcta aattaggagc aaaagaagtt gtagctgtag
    10981 atttagatga agtggctgta aaagttgcaa aagaaaatgt tcttgaaaat aaagttgaaa
    11041 aaagtgtatc tgtaatgcat ggtaatttaa ctgatgtaat aaaagataaa gcggatgtaa
    11101 tagttgcaaa tataatagct gatataataa agatacttgc aaaagatgtt caaaacttta
    11161 tgaaagaaga tgctatattc atatcttcag gtataatttt ggataaagta gaagaagtta
    11221 aagaaagctt aattgaaaat ggatttgaaa tagttgaagt tcaaaaatta ggtgaatggt
    11281 cagcaatagt ttctaagctt aaaagataag ggatatggtg acgtataatg gatagatttt
    11341 ttgttgaaaa aaataatata aatctacaag ataaaacttg cactatagaa ggtgaagatg
    11401 taaaacatat ttcaaaagtt ttaagatgca aactaggtga gaaacttgaa atatgtgaca
    11461 agaataataa tgagtatatt tgtgagataa tgaatataga taagagtata gttaatttag
    11521 agattttaga aaaagttgat ataaatagag aatcagaact taaagtaagg ctataccagg
    11581 gacttccaaa agctccaaaa atggaaatga tacttcaaaa attaactgaa gtgggcgtag
    11641 aagaaataat attagttcaa actaagagaa gtgttgttaa agttgatgac aaaaaagaag
    11701 ataaaaaatt tgaacgttgg gaaagaataa tttatgaagc agcaaagcaa agcaaaagag
    11761 gtaagatacc aaaattaaga ggtgttttga gttttaaaga agctttagaa gatatgaaaa
    11821 aaaataatgt aaatatttgc ccttatgaaa atgaaagaac agtttcaata aaacatgcac
    11881 taaaaaaatg tgattctaat atagatagtg ttggaatttt tataggtcca gaaggtggat
    11941 tttctgaaga agaaatagaa caaatacaaa aaaataattg taatgttgta tcattggggc
    12001 ctagaatatt aagaactgag acagcttctg ttgtagcatc aacaatagct ttgtatgaac
    12061 taagtgattt aggaggagaa aaataaattg aaaaaagtag ctttttatac acttgggtgt
    12121 aaagttaatc aatatgaaac agaagctatg ctagaactat ttgagaaaga tggttatgaa
    12181 caagtcaata gtgaagagta tgctgatgtt tatgttataa atacatgtac agttactcat
    12241 atgagtgata gaaaatctcg tcaatatata agacgagtta aaaagaagaa tccagatgca
    12301 ataatagccg ttgtaggatg ttattctcag gtatctccag aagaaatttt ggatatagaa
    12361 gaagtaaatt tagtcatggg tacaaatgat agaagaaaga ttgttgaaga aattaaaaag
    12421 ataaattcaa gtaagaaagt tagtacagtt gatgacataa tgaaagttaa agcttttgaa
    12481 gaaatagaaa tcagccaaac taatggaaag actagagcat ttatgaaaat acaagatgga
    12541 tgtgacagat tttgtactta ttgtatcatt ccttatgccc gaggacgcgt tagaagtaga
    12601 gatattgata gtatagtcga tgaagtgaaa aaattggcta ataatggcta taaagaggtt
    12661 gtattaacag gaatacatgt tgcctcttat ggaaaagatt taaaggatag ggatattaaa
    12721 cttcttgatg taataaaaca aataaaccag atagaaaaaa tagaaagaat taggcttagt
    12781 tctgttgaac caattttatt tacagatgaa tttgtaaatg aagttttaaa gatggataaa
    12841 gtttgtcctc attaccattt atcacttcaa agtggatgtg atgaaactct aaaaagaatg
    12901 aatagacgat acacaacttt agagtacaaa accattgtag atagattgag aagtaaaatg
    12961 ccagatgtgg caataactac agatgtaata gtaggttttc ctggagagac aaatgaagag
    13021 tttaaaaaaa cgtacgagtt tttaagagaa atagagcttt cacaaatgca tatttttaaa
    13081 tattcaccaa gaaaaggaac tccagctgca actatggaaa atcaagttga ccctcaaatg
    13141 aaacatttta gaagcgaaca acttttaaat ttaagtaaag taaattttaa taaatttgca
    13201 actaaattta tcggccgtga gttggatgtt ttatttgaac aaaatataga aggcaataaa
    13261 tatgagggat taacttcaaa ttatataaga gttgttgttg aaagtgacaa aaatatacaa
    13321 ggtcagatat taaaagtgaa aataaatgat gtaaaagatg aatatgtaga aggaattttg
    13381 ctataacatg tagaaattat aatatttata agataggagg tggaaagaat ggattgtatt
    13441 ttttgtaaaa tagcgaacgg agaaatacct tcaactaaag tttatgaaga tgatagagtc
    13501 ttagctttta atgacttgaa cccagttgct ccatatcata tattagttgt acctaaaaaa
    13561 cattatgata gtttaattga tattcctgat aaagagatgg acattgtgtc acatattcat
    13621 gttgttatta ataaaatagc taaagagaaa ggttttgacc aaactggatt cagagtcata
    13681 aataattgtg gaagtgatgg agggcaagaa gttaaacatt tgcactatca tattttagct
    13741 ggtaaaaagc ttcctaatta cgaagcggga caaaactaaa ctaaattatt gaaaaattga
    13801 gttccttctt gataaaaata cttgataaaa gtaaaaattt agtttataat aaacaaagtg
    13861 ttaggttgta atgcatatta tgcaaaaaga acatagttac agtcccagca taattgcgct
    13921 attggaggga gggaaaaaga aatgtcagaa gtaagagtta gagagaatga aacattagac
    13981 agcgctttaa gaagattcaa gcgtcaatgt gcaatgtctg gcatcatgtc agaagtgaga
    14041 aaaagagagc actatgataa gccaagcgtt aagcgtaaga aaaaagctga agcagcaaga
    14101 agaaaaaacg ctaagaaata gtagtaaata agaataaaga ggtgaagggg atgtccctta
    14161 agcaaaagtt acaagaggat ttgaaatctt cgatgaaaaa caaagataca gtgagaaaat
    14221 ctgtagtaac tttaataaga gcttctataa agcagtatga ggttgataat agggtcgagc
    14281 ttgatgaaga tggaattatc gatgtaatag caaaacaatt aaagcaacgt agagatgcat
    14341 tagttgaatt tgaaaaagct ggaagagaag acttaataaa agaaacagaa ggtgaaattg
    14401 aagttttaaa agagtaccta cctcaacaat taagcgaaga agagttagaa gaaattgtaa
    14461 aatctactat atctgaagta ggggcaactt ctatgaagga tatgggaaaa ataatgtcag
    14521 ttatccaacc aaaagttaaa ggaagagctg acggaaaact tataaataag ttggtaaaac
    14581 aaaacttaca atagattgat atagcctaga gagaaatctc taggcttttt ataatttttg
    14641 ggaataacga gtcatatttg agcatatcta taatcaaaga aaattttgaa agaaggggaa
    14701 aaatttgatt atatcagtaa aagagattag gaattttttg ataaacatat ttataccact
    14761 aataataggt tacttaagta gcatcttaac aatgattatc agtggaacag atatttcaac
    14821 ctattattta caactcacaa agccaagctt tgcaccacca gcttttatct ttcctatagt
    14881 ttggactata ttgtacatat tgatgggtat atcttcatac ctaatattaa gaaagggtta
    14941 caacttacct aaagtaagag atgcaatttt ttattatgga ttacaacttg tattaaactt
    15001 tatttggagt atactatttt ttggctttgg acttagattt acagctttga tagatataat
    15061 aataatgatt ttggtttcta taataatgat ttcgaagttt tctaaaatag ataagagatc
    15121 tggatacata aatttgattt atttaatgtg gcttgtttat gcaggatttt tgaactattt
    15181 tatatggttt attaataaat agaatgtaaa gattttgtaa attaccctgt acttttaata
    15241 atatcataac aaacgaaaaa taaatagact attaaaaata atcattttca tatacaataa
    15301 tattaattta aatacaataa atttataaaa attgtattta aagattaaca tattaaagta
    15361 ttagaattgg aaaggcttga agggagaggt ttttatgcaa ataatagtat ctgggagaca
    15421 aatgaaatta actgacggaa taaaaggata cgtagatgga aaattaagta ggttagaaaa
    15481 ataccttgat cctgaatcag aagtgaaagt aacagtaagt gcgaaaaagg atagacaaaa
    15541 agtggaggtt acaataattc ctattaacgg tcaaataata agagcagaag acgtagaaga
    15601 tgacttatat gcagcaattg atattgtttg tgacaaatta agtagacaag ttgtcaaata
    15661 taaaactaaa gttaaagaca aagttcaaaa taataagagc ataagatttg aaaatcttga
    15721 ctttatagat aatagcagtg aatttgatga ttatgattat gatgatgaag aagatgaaaa
    15781 tatagtaata gaaagaagaa aaaaatttaa tgtaaaacct atgagttctg aagaggcgat
    15841 acttcaaatg gaactagtag gacacaattt ctatatgttt agaaatcaag ataattttga
    15901 aataaacata gtgtataagc gtaaagctgg tgggtatggt ttaatagaac aagactaata
    15961 aaaaaattgt atacgtttag attttatata agaaaaaata aaaaggtaat catgttgatt
    16021 ttttatcaat atgattacct ttttattgtt taaataatat attagatatt aggtatttat
    16081 tatataaaaa ttttaaaact aatctgttac aacatcaata aagtgcataa atacctattt
    16141 caagcataaa attaattaag ggggtaatgc ttatgttaga aatatccact gatttacaag
    16201 taaatcaacc agttattact gttacaagta atacgtttat tagtatagaa aattatctat
    16261 caatattaga atatgaagtt gatttaataa gaataaagac taaagttaaa acaattaaaa
    16321 tatctggaga taaactttca cttaagtata ttactgatag tgagataggt ataaaaggaa
    16381 ttatctacaa tgttgagtat gttgattagg aggagataca cttgataagt tttataaggg
    16441 gatactatgt tatagttgtg gaaggagtag gactcgaaca atttttaaat catcttataa
    16501 gaaatgggat aagtgtttac aatgtaacta gaataaaaaa tactaaaatg gaatttcata
    16561 tagatagaca agatataaaa gaatttaaga atgtttatag aggaagtaag tttgatataa
    16621 aggtaaagca aaaaactggg gttcctttta ttatcaaaag agtttataag cacaaaggta
    16681 tgtggatttg tgctcttgtt tctttatttt tattgatgtc tacatcacag tttgtgacag
    16741 atgtatatat acaatctcct gaaggtataa aaaaagaagc tcttagaaat gagctttaca
    16801 aggttggagt aagaccagga gtctataaga agagtattga tagaaaagaa gttagagacc
    16861 atatgatgtc aaaatttaat gatgtggcat atttatctat caatgtaaaa ggaacaaata
    16921 tatttgttac tgttacaaaa aaggcagaat ccctaaaatc tacagaccaa tcaaattatt
    16981 gtaatgtaat tgctttaaaa aatggtataa tagaaaaggt aatccctaga agtggtaaat
    17041 cagttattaa atctggagac atagtccaaa agggagatgt cttattaaat ggagctaata
    17101 ccaaatcaat accagaagta tgggcaagta ctttttatga gtccacaaaa aaggcaagtt
    17161 atgtagatac tgtaaataaa aaaactggag aaaagaaaaa tatatataca ctaagttttt
    17221 atgataaaga gtttacaatg agaaaaaata taaaatataa agattatgtt gttgaaaata
    17281 aagaaaaaaa actatcaata ggaaactata cttttccaat aaagataaaa acgagtactt
    17341 tttatgaaac aaaaaaggtt gaagtaaaaa agaataaaga agagttaaaa aaagaattat
    17401 ctgaaaaagc tttaaaagag ttagaataca taatcccagc ttcggctaga ataatagatg
    17461 taaaacataa ctttaaggtt aataaaaata tgttagaata tttaataaca gttcaaacta
    17521 gtgaaaatat tgctaaaatt tatcctctta gtaagtcaga agcagaaagg tttataaagg
    17581 aagaaagtaa accagatgaa ggtgaagagg aagttccttc caatccagaa aaaagaccat
    17641 taaatgatat aagaaatgag tttgatgagg ataataagga taataaagac caaaacaatt
    17701 ctgatgaaaa taatagtaat caaaatagta ataatagtca aaataataat agcaataaca
    17761 actaataaaa ttaaacttaa aatcttagga ggactaattc aattgataat acaaaagaaa
    17821 tttacaatag cagatggtaa ctttgaaaga gagttatttg gcaactttga tgaaaatgta
    17881 aaattaatag agaagacttt aaatatagat gtaatactta gagaaggtaa tataatttta
    17941 attggagaag aaaaaaatgt agattctgca ttaaaactta tgaatgaatt acaccaaact
    18001 gtatcaaatg gtaagcattt agataaacag agtatttctt attcattatc tcttttgctt
    18061 gaaggaagtg aacaaaaaat aaaagaactt gaggggacaa ttgtcataac tcaaagggga
    18121 aaagctgttc aacctaaaac tttagggcaa aaagaatata taaaacttat agaaaataat
    18181 gatataactt ttggtgttgg acctgctgga acaggaaaga cttatcttgc agttgctatg
    18241 gcagtaaaag cttttaaaag agatgaagtt agtagaatta tacttacaag accagctgtg
    18301 gaagcaggag agagtctagg ttttttacca ggagatttga aggacaaagt tgacccatat
    18361 ttaagaccac tatatgatgc cttatttgaa atgttgggtg ctgataaatt taataagtat
    18421 ttagaaagag gtactattga ggttgcacca cttgcattta tgagaggtag aacacttgat
    18481 aattcattta taatattaga tgaagctcaa aatactactt ctgaacaaat gaaaatgttt
    18541 ttaactagac tgggatttgg ctctaaagct gtagttacag gagatgtaac tcaaacagat
    18601 ttaccacaaa ataagaagag tggtctaata caagctacag agattcttaa aggtgttcca
    18661 ggtattgggt ctataatgct aacagataga gatgttgtaa gacatgagtt ggtacagaga
    18721 ataataagag cttatgagaa gcacgacaaa agagaagaat ttaaaaaaga agaacgtaaa
    18781 aaagcgaaaa tgcaggaaaa gactttcaaa agaaagtaaa agtgtagtaa tatagatata
    18841 tagcattatt aatgaataaa aagtaggtgt agtgaatgga tttgatacta gatgatagac
    18901 aagataaatt agaagtaagt gaggagttaa ttgaaaagat aaaggatata atcatagaat
    18961 gtttagatta tgaaggatat gatgataatt atgaagtgag tctttctttt gtagataata
    19021 aagagattca tgaattaaat agagaatata gaggagttga tagagtgact gatgttttat
    19081 catttcctct tttaagtgat gattttgagg atgttgagtt agaagaagaa tccctaggtg
    19141 acatagtagt atctcttgaa agagccttag aacagagtat agagtataac catagttttg
    19201 aaagagaagt atgtttttta atatgtcata gtatgtttca tcttttaggt tatgaccacg
    19261 atacagatga aaatacaaaa gaaatgagag aaaaagaaga acacatatta aataagttaa
    19321 acataacaag ggagtagttt ttatgaaacc agaaaaaact cgtcaaggta taattaaagc
    19381 atttaatgca gctatagaag ggatattgta tacatttaaa tttgaaagaa atatgaaaat
    19441 acattattta ggttcagttg ctgttcttat aataagtttg ttttttaact tttcaaaatt
    19501 agaaatgata atgttactta tgtccatatg tttagttgta gttgctgaaa tgtttaatac
    19561 agctatagaa aaagctgttg atttagtaac tgatgaatat catgttcttg ctaagatagc
    19621 aaaagatgtt gcagctggag gcgttctagt ggcagcacta aattcagtag tagttggata
    19681 tattttgttt tatgataaat tgacagatat atctggaata ttaatttata aaataagaga
    19741 atcagaatta catataacct tgatatgtat attattggtt cttatagctg ttgtagttgt
    19801 aaaagcattg acttctacag gcactcccct aaaaggagga atgccaagtg gtcatgctgc
    19861 cttagcattt gccatagcaa ctgcaattac acttatgact gaaagagttg ttgcctctac
    19921 tcttgcatat ataatggctg tattagttgc ccaaagtaga attgaaggaa aaatacatac
    19981 attttgggag actattgctg gagctttatt aggtgtgttg atagctatat tagtatttca
    20041 gcttggaatg ttctataatt aaaataaatg tacataaaat atttaaaata aaacaaatct
    20101 gtgctaatta aatttttatg atataatgta gagtattaaa taaaaataaa ttttaattat
    20161 tgtatacagg aggagttgtt gtggataaca tagaattgtt aaggttggca gaagatgcca
    20221 gacaacattc ttatgctcct tactcaggtt ttagagtagg tgcagctctt ttaactaaga
    20281 gtggtaaagt ttatactgga tgtaatatag aatgtgcatc attaggtgga acaaattgtg
    20341 cagaaagaac agctattttt aaagcaatat cagaaggtga taaggatatc cataaaatag
    20401 ccatagcaag tgataactca gaaaataatg agcaaacata tccatgtgga atatgcaggc
    20461 aggtaattat agagtttggt tctgatataa aaataataac gggatatact aaaggagagg
    20521 tgtttgagca taatataaag gatttgttgc caaactattt ctctggaaat gatttaaaat
    20581 aaatttaaaa gtataaataa atttaagtaa ttaaaacaag aagataaaaa gttaggagac
    20641 agttaatgtt caaatcaggt tttgtcagta tagtgggtag accaaatgta ggaaaatcta
    20701 ctttaatgaa taatgtagtt ggagaaaaaa tagctataat gagtgataaa cctcaaacta
    20761 caagaaatac aatacaagca gtatatacag atgaagaaat gcagatagta ttcttagata
    20821 caccaggtat tcacaaacca aaaaataaat taggtgaatt tatggtaaag gcagcaacag
    20881 aagcctttaa aaatgtagat ttaatattat ttgtagttga tgattctaaa aaaataggtc
    20941 ctggagatag aaagataata gaggatttaa gaagtgttaa gacaccaata attttagttg
    21001 taaacaaaat agaccaacta gaccaaaaag atgagctatt tgatataatc aaaatgtatg
    21061 atagagaagg tatttttaaa gaaatagtac caatatcagc attaaaagga aagaatacgg
    21121 ataccttaat aaaagttata caaaattatt tagaagaagg gccaaagtat tttccagatt
    21181 atatgattac agaccagcca gaaagagtat taatagcaga gcttataaga gaaaaagttc
    21241 ttcactatct taatgatgaa atacctcatg gtgtagctgt tgaaattgaa aaaatgaaag
    21301 ctagaaaaga taaggaaata gtagatgtat ctgctgtaat atattgtgaa agagattcac
    21361 ataagggaat aataatagga aaaaatggta ggaaactaaa aggaataggt aagtcagcaa
    21421 gacaagatat agaattactt ttaggttcac aaataaatct tcaattatgg gttaaagtca
    21481 aagaaaattg gagaaattta caaaattata taaataattt tggatataat gataaataaa
    21541 ggtgggttat atggatagga aactggatgc ttcccaagat ttattatatg aagtcaaatc
    21601 attaattgat aataataaag tattggagtt aagagaatta atagaggaat atcatataat
    21661 tgatatattc gatataatgg aaaatttaga ggaagacatg aaaattcaat tatttgaagt
    21721 acttccttta gatatggcat cttctatatt agaagaaggt agtgtagagt tttttattag
    21781 tattttatct aaactggatg tagaacactc gaaaaatata cttgaactta tgtctcttgg
    21841 agatatggca gacaagttga gtgaacttga agaagaagaa agagaacaca taataaatct
    21901 tttgaatcaa gagaatgccg attatgtaaa agagttacta ttctatgatg aagattctgc
    21961 tggtggaaca atgaccactg gatatatttc tataaataaa gatatgacag ctcttgaggc
    22021 tatagaccat atgagagaag aagctgaaga agctgaaact atttattata tatatgtggt
    22081 tgatgatgaa gaaaaactag ttggagtatt atcattaaga gaattaataa tagctagaga
    22141 tgcaaatata gtagaagatt tgatgagtga aaatataatc tctgtttatg tagatgaaga
    22201 tagagaagaa gcagtaagat tagtatctaa atacaattta atagcaattc cagtagtaga
    22261 tagacaagaa aaattaaaag gaataataac agtagatgat ataattgatg taatggaaga
    22321 agaagcaact gaagatatgt ataaatttgc aggaagttca gaacatgaaa gagaagttgc
    22381 tgaaaaagaa aaccctacat tgagagagca aataatatca gcacttagag gaagattacc
    22441 ttggttaatt attaccttag ttggagggtt gttggcttcg ttaatacttt ctaatttaga
    22501 ctatataatg aatcctgtat atgctccatt agtatttttt atacctgtag taattggtat
    22561 gggtggaaat attggaactc aatcatcatc tgttacagta ataacattat ctaataagga
    22621 tttaaatttt agcaatgtgg ttagagaagg tattgtaggt attattacag gtcttttgtg
    22681 tagtataata acaggtattg taatttactt tgttatgaga aagttagaca ttgtattaat
    22741 tgtttcaata tcattattta taaatatggt tttaggtgca acaataggtg cgtttatgcc
    22801 tgttttatta aaaaaaatgg atgcagaccc atctacagtt tcatcaccaa ttatatctac
    22861 agctcttgat ataactggta tagcagttta ctttataata acaactgcat tattgtcaaa
    22921 aattgtttaa taactttatg tatgatttat cctcttctta ctaaaaatat aagtataaat
    22981 gtaagaaggg ggttttttaa aaatgaacaa tatcagttgg gaagttttta aaaaaacagg
    23041 tagtatagat gcatatttat atttctgtga ttgcaaaaac atacatgaag aaattgaaga
    23101 agtaagggag aaaaaagaag acgatgataa tcctgaacac ccaggggata gtacttaaag
    23161 ctataaggta taaggaaagt gacataatac ttacactatt tacaagaaag cttggaaaag
    23221 tatctgcaat agcaaaagga gctaaaaaaa ataagagctc tttgctttca tcatcacagt
    23281 tattttctta tagcaatttt accttaaaaa aacaaggtaa tatgtacaag gttactcaaa
    23341 gtgaaataat aaaaagtttt tataatatat cctatgatat agaggcattt tcttatgcta
    23401 catatattac aaaattagtt gaaaattcta tacttgaaaa tcagactaat aataggctat
    23461 ttatattact tgcacaaact ctgtatttgt atacacagga taatacagac aataggttta
    23521 taacagcagc atttgagtta aaatttttag actatatagg atttaaacct atagtaaata
    23581 aatgcattaa ttgtgaatgt aaaattttac aaaattctgt gtttaatatc tacgaaggtg
    23641 gtatactatg tagtaagtgt agtaaacttt ttgataataa tataaaatta gacttaacaa
    23701 ctataagtct tatggaatat gtattaagaa atgatatttt aacatgtagt aaagctaaag
    23761 tatcaaaata tataacacat gaacttgaga atattctaga caagtatttg aaagtttatg
    23821 ttgataatat taattttaaa tcattacacg tattgcaaag tgtaaaaaat aataagggag
    23881 tggataatga tgagtagtaa tataactatt gaaatggttg attcagtctt tgaaagagtt
    23941 ccaggagcaa cttatgcaga agcaaaagaa gcattagtaa tgtgcaatgg agatgtaatt
    24001 gaggcagtaa tatatttaga aaataaaaaa aatacttgta attgtaaagc aaaatctgct
    24061 aaagaaacta tggaagaagt atttggcaaa gatggagaaa atataaagtt tcaattaaaa
    24121 gagcttctta gaaaaagtag tgtagtaaga ataatagttg aaaaagatgg aaaaactatg
    24181 atgaacattc ctttaacagt tggagttgtt ggattagctt ttgtaccatt agctacttta
    24241 gttggattat cagctgctgt tataagtaaa tatcgtataa aagttcaaaa tgaagaagat
    24301 ggagaaattg tagatttagg tgaattgaat gaagaaaaac ttcatgtttt gaagaatatg
    24361 atagctaatg cagcaaaaga tgtgaaggat gtagtagttg acaaaaaaga agatgacaaa
    24421 gatgtaactg ctgaccttat gaaagaagaa tctgaaaaga ataaagaaga cgaagacgaa
    24481 aataaataga tataaagtga cttttaaaaa atgaagagaa gataattata agaattatca
    24541 ctaacattga caaattttat agaatttgat attctaaaag ttgtagaaga actattaaaa
    24601 tagcctttcg tgtagacgag aggctttatt gttataggag ggatttttat gaattttcaa
    24661 gagatgatac tggcgttgca aaaatactgg tcaaagcaag gttgtatcat gatgcaacct
    24721 tatgatattg aaaaaggtgc aggaactatg aatcctaata cttttttaag atcattagga
    24781 cctgagcctt ggcaagtatg ctatgtagag ccttcaagaa gaccagcaga tggaagatat
    24841 ggtgaaaacc caaacagact atatcaacat catcaattcc aagttatact aaaaccttct
    24901 ccagataata tacaagaatt atatttggaa agtttaaaag agattggaat tgacccaagt
    24961 gagcatgaca taagatttgt tgaagataac tgggaagctg ctacagttgg tgcttgggga
    25021 cttggatggg aagtttggct agatggtatg gaaataactc aatttacgta ttttcaacaa
    25081 gtaggtaata ttgaatgtga gcttgagaca ggagaaatta catatggatt agaaagatta
    25141 gctatgtaca ttcaagaagt tgatagtgtt tatgatttaa aatggaatga taaaataact
    25201 tatggtgaag tatttaataa agctgagtat gaaaactcaa tgtacgcatt tgaattatgt
    25261 gatgcagata tgttgtttaa tctgtttgat atatatgaaa aagaagcatt aagacttatg
    25321 gaaaatggac ttgtaatacc ttcatatgac tatgtgttaa aatgctctca tgcatttaat
    25381 acattagatg caagaggtgc aataggagtt agtcaaagag cttcttttat aggtagagtt
    25441 agaaatatgg caaagactgt tgcagaaact tttgtaaaac aaagagaaga aatgggattc
    25501 cctcttttaa aggacggtga caaataatga aaaattatct tttatttgaa atcggtgttg
    25561 aagaaatgcc atcaagattt gtaggcagta cactagaaca attaaaaaac aatcttagta
    25621 aattatttga tgaaaataga atagcttttg ataaaataaa tgcgtatggg acaccaagaa
    25681 gacttacttt agtagtcgaa ggattgagtg atagacaaag tgatttagaa gaggaaataa
    25741 agggtcctgc taagaaaatt gctgttgatg cagaaaataa tttaacaaaa ccagcactag
    25801 gatttataaa aagcaaagga ttaagcgaat ctgacatata ttttaaacaa gttggaaaag
    25861 aagaatatat atttgcgact ataaagcaag atggaaaaga aacttcagaa gtcctaaaag
    25921 acatattacc agagactata aaatctgtag tgttcccaaa agctatgcgt tggggaggaa
    25981 aaaatcttaa atttgcaaga cctataagat ggatagttac attgttaaat gataaagtgt
    26041 tagagtttga tttagaagga ataatatctt ctaatgtaac aaaaggtcat agatttttag
    26101 gtaaaagtac aatagaagtt gattctatag aagattattt tgaaaaatta gaaaaaaatt
    26161 atatagtttt agaccaaaac aagagaaaag aaatgataag aaaacaatgt atagaagtag
    26221 caaattcttt gggtggagaa gttgaatttg atgaagattt acttgaagag gtaacttact
    26281 tagttgaata tccaactgct ttttatggag aatttgatgt ggattatata aaactaccaa
    26341 aagaagttgt tataactcct atgaaacaac atcaaagata ttttccagta cttaaagaag
    26401 gaaaactgtt acctaacttt atagctgtta gaaatggtga ttcttacaga atagacaatg
    26461 ttaaagctgg aaatgaaaaa gttttagaag caagacttgc agatgcttta tttttctata
    26521 gagaagatac taagagaaac ttagaaagct acatagaaaa attaaaaact gttgtattcc
    26581 aagtaaaact aggaactatt tacgacaaaa ctctaagact tgaaaaatta agtgctgata
    26641 tacttgatag tttaggttta gtagatgaaa aggcagatac tataagggct gctaaattat
    26701 ctaaggcaga tttagttact ggaatggtag gagaatttga tgaattacaa ggttttatgg
    26761 gaaaagaata cgcaaaagtt ggaggagaga atgacgttgt aagtgaagct atttttgaac
    26821 attatcttcc aagatttgca ggagatatac ttcctaagac taatgcaggg gttgccttat
    26881 caatagctga taagttggat tcaatagcag gtttctttgc tataggaata caaccaacag
    26941 gttctcaaga cccatatgct ttaagaagac aagctttagg tatacttagt atacttatgg
    27001 acagaaaagt tgctgtagat ataaaagtac ttattgaaca tgctttagat aattattctg
    27061 aattagattt taataaagaa gaagttgttg agcaaataat gaatttcttc aatgagagag
    27121 taaaaaatct atttaaagat ttaggaataa gatatgatgt tgttgatgct gttttaagtt
    27181 ctgatataaa tgatgtttct gatatgcata tgagagcttt agagcttaat aattggctag
    27241 aaaaagatga attagtggag atgcttactg catttaatag agtttctact ctagctcaaa
    27301 aagcagaaag cgataaaatt gatgaatctt tattaaaaga agatgctgaa attaaacttt
    27361 acaatgaatt taaacaagta aaggtaaaag ttaatgaatt attaagggat aagaaatatg
    27421 gtattgcttt agatgaattt gcatctttaa gaccagtagt tgataattta tttgatacag
    27481 ttatggtcat ggataatgat gaagctatca agaacaatag acttgcttta cttaaacaag
    27541 tatatgatac tatgctagaa atttgtgatt tatctaaaat tgtttataaa taaagtttaa
    27601 attatttata agaaataagg ctgtctcaaa atagagatag tcttattttt cattgttgag
    27661 atgatgaaaa tatatttgat ttcatagaaa aagagtgtct cataaactaa aaagttcatt
    27721 ttgagacacc aaactttttg tgagtaaata taataaatta tattttatac ttatcagtta
    27781 ttttgtttaa aatatcatca tttgaattta cccattcata aaaattgcct gatgatgcat
    27841 ccgatatttt ataaagcatt tcaggaatat cactagcttt aaaatctcca agtgattttc
    27901 ctaatctagt cttacctatt tcaaaactgc ctccaacaaa taattcaaat gcatctgtag
    27961 atataccatc aacattcttc tttttaccgc ataaacctat actaccaact tgatgaacgc
    28021 cacatgaatt taaacatcct gatatatata atttaggtgc tttatttaaa atttcttcat
    28081 tattttgtaa tctaaaataa tctatgattt catgtaacat tttctgacta ttttgtatac
    28141 ccatttgaca aattggaaca cctatgcaag atatactttg ttctaattga gaattacaac
    28201 taatagtttt acttatttct aacattcttt ttgcttcatt tccatttaaa tttaaaatat
    28261 ataatccctc tgtcattcct agtctaatca taggattttc aatattatct aattctttaa
    28321 gtaaagtact taaatccttt gttaacaaaa tacctccaac tggatgtata tacacagtgt
    28381 ataaaccatc ctgtttttga ggtattagtc tagaatcctg tatatctatt ttaactccag
    28441 gtttagagta gtctatttct tcaatattga gttcaagact acccttttct ttttctaatt
    28501 tatagtattc tttaaatttt tctaagaata attcttcacc aagtgattcg accatatatc
    28561 taaccctagc tctattttta ttttcataat tgccataatc cataaagaat ttaatcatac
    28621 cttcaacaca atataatgca tctttcggct ttataagttc atcaagttct aaagcaactt
    28681 tgggttgttt acctagacct ccacctaaga aaactctgaa atatggttca tcatttttca
    28741 aagtagcaac aaatcctaag tcttgtatac tacaatgagc agtatctgtg taacaattag
    28801 aataagaaac ttttaatttt ctaggtaaat ggtatgtagt agcatttttg ataaaatatt
    28861 tatcagtagc aactgcatat ggagtaacat caaatgcttc actagggtca actccagata
    28921 atggagataa tccgacattt ctaggataat taccaccacc acctctagtg aatatatcat
    28981 tttttattcc ttcttccatg atatcaacaa ttgaatttat agataaatcg tgtaattgta
    29041 tagcttgtct agttgttaag tgtatcttat ctaagttgtg tttaatagct atttcataga
    29101 ttttattgag ttgatgctga gataaaactc cagcagatac tctaagtctt accataaaag
    29161 attttttgtc tctttgagca taaacaccat atccaccaga aaatccctta tattgcattt
    29221 tatttatttc tccatttaaa aattttgaag aatattcttt aaaattaggt agttcattta
    29281 acaatatctt tttaaaatta tccattattt cacctcattt ttagtcttgt ataaaaatat
    29341 atctattgta taatagatat atatattatt atctagtcag gataaaaata taacagaata
    29401 attctattaa aa
//
LOCUS       DAEPXF010000031        29340 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain HB27
            SAMN13376389-rid12479423.denovo.033, whole genome shotgun sequence.
ACCESSION   DAEPXF010000031 DAEPXF010000000
VERSION     DAEPXF010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13376389
            Sequence Read Archive: SRR10518432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 29340)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29340)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001069624.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001069624.1
            Genome Coverage       :: 315x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/15/2021 01:54:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,165
            CDSs (total)                      :: 4,076
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 89
            rRNAs                             :: 10, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 10 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 19 of 69
            Pseudo Genes (incomplete)         :: 44 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 10 of 69
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29340
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13376389-rid12479423.denovo.033"
                     /strain="HB27"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="China: Hebei"
                     /collection_date="2017"
     gene            complement(15..512)
                     /locus_tag="KRL67_001239"
     CDS             complement(15..512)
                     /locus_tag="KRL67_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH1522135.1"
                     /translation="MFPILLFDNNLNDFTIIVGAFFSLLIISLISGLLATLILPEKWV
                     FTVTRGGLFISLLITVLGGIWPMIGRFYPKEYKSTDIFKRSMAIEGLFEWLGLLCLIL
                     LIEIFARQSEFCEYIVSLGKSLLILHSIPFYPFECFGGKRIWNYSKILSIITIVISIG
                     MLYLF"
     gene            complement(555..728)
                     /locus_tag="KRL67_001240"
     CDS             complement(555..728)
                     /locus_tag="KRL67_001240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH1522136.1"
                     /translation="MDNIIIREMVDDERRFVWELGKSSFGLVESLAFRKPKKAFIASG
                     FHLWSTSAQKSNS"
     gene            complement(965..3235)
                     /locus_tag="KRL67_001241"
     CDS             complement(965..3235)
                     /locus_tag="KRL67_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HBH1522137.1"
                     /translation="MLSDAKNNRYGLSIIAVISILVITISCLIYGSRLNKTLGEETNQ
                     YLSEIANQSVNVLKKQINGDIKLLESISIFIEGEESFEVDNILSILKRQAINSSFKRM
                     GVILPNGTAYTTDGYIEDFSKRDYFLKSMQGEVVITGKLKDVIKDDKNTNSNINVYSV
                     PIYKDNEVKGVIFATHSTKLYEKILSVPTFNGKGYSYIVRSNGDIVLNPSSKNANSTK
                     ENLFSYIGRSDSISKSTLNNMKSNIKNFKSGSLSYNMSGNGYYLSYAPIGINDWYLFS
                     EVPKTAISEKSYAIIKLTLSACVVLIIIFTALTMYILFIQKKSKEKLEDFAFKDNVTG
                     IGNSNKFNLEGGKFLSSHEKRNLVLIYFDIDKFKLVNDRFGYEEGDRVLKGIAEIVKS
                     IFKEQSVFSRISNDNFAIIFEKKNDRESVVEICELIRKKLSMIKTSLGVELNLIPSIG
                     VYFVEDGETNISTCLDKAMIAKTTVKRKYLKVYEIYAENLKETLIEERDIEQEMHDAL
                     KNEQFKVYLQPKIELSTTKIVGAEALVRWQHPKKGLISPGVFIPIFEKNGFITELDMF
                     VFTQVCKNFKRWENESFPTFPISINLSRVHLENPDFISELERITKEYEIEPNLIEIEL
                     TESAIFDNTKILFKIMQTLKSVGFKISMDDFGSGYSSLNMLKDMPIDVLKLDRQFFIT
                     VGDAKKSQIVVSSIVQMAKQLDIKVVSEGVETVEQAEFLRFIGCDMAQGFLFARPMPI
                     EEYEKLILDRYSVKGK"
     gene            complement(3506..4960)
                     /locus_tag="KRL67_001242"
     CDS             complement(3506..4960)
                     /locus_tag="KRL67_001242"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HBH1522138.1"
                     /translation="MKLNDIIQGLDIINVKGELNIDINNVQYDSRKVTKGTLFICIKG
                     FVSDGHKYIKDAIEKGASAFLVEEDVEIKGCTFIKVKDTRKDMAKVADNFYNHPSQKF
                     NVIGVTGTNGKTSITTILNEILTLNKNKVGLIGTIKIFDGEKDIVSNSTTPESIDLQY
                     HFNNMLDNGCDYCAMEVSSHSLALNRVDETDFKLGIFTNLTPDHLDFHKDLDDYRKAK
                     EKLFFKTTMANIINIDDEGGKKIYENIKGINVPCYTYGVDTKADFMARDIKSDSDGVS
                     YRLITPSYEEVIFIPVPGMFTVYNTLAVIAACYVLGIPKPIYKEGLRLSNGVSGRFET
                     VPNDKGISVIVDYAHTPDALENVLKTTQQFAEGKIISVFGCGGDRDTEKRPLMGSIGQ
                     KYSDLCIITSDNPRTEEPELIIKDILEGIDKKKENYHVVVDRKQAIAEAISMAKKDDV
                     VIITGKGHETYQIIGKVKHHFDDKEVANECLSKM"
     gene            5124..5993
                     /locus_tag="KRL67_001243"
     CDS             5124..5993
                     /locus_tag="KRL67_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBH1522139.1"
                     /translation="MDNKIGYLNDNFKIFNIRDKKDIQFEYHNHDFNKIIIFINGNVT
                     YFVDGIPYKLKPWDILFISNNEIHKPVIDSNVFYERIAIWISPEFMKKNSDANSDLEK
                     CFKIATENKCNRLRLNIHDIESIKSFIEQINISESNTEFGNEILRNALFIQLMIFLNR
                     IFLKNENMESTEDVFYDETIKKVLNYINSNIDDKLSIDSISSEFFISKYYLMRKFKSH
                     TGTSIHNYIVQKRLILAKSLITNGHLMSEVCNKCGFNDYSSFVRAFKKNYGVSPKNYI
                     NNKNPLDITLLDE"
     gene            complement(6207..8279)
                     /locus_tag="KRL67_001244"
     CDS             complement(6207..8279)
                     /locus_tag="KRL67_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HBH1522140.1"
                     /translation="MKKVFGVLQKVGKSLMLPVALLPAAGILLGVSNALAGDALLAHF
                     PALANPTFQLIISIMESSGQIIFNNLPLIFAVGVAVGLTEGEGVAALAAIVGFLILNT
                     SMGVMAGVTEDMIDNSMYASIVGIPTIQTGVFGGIIIGIVAAYLYKKFYKIELPQYLG
                     FFAGKRFVPIITSLVAIAIGIILSFIWPPIQEGLLSFSKNVIEANQTLAAFLEGSLER
                     LLIPFGLHHVFHNPFWYQFGEYVNKAGELVMGDQKIFFAQLKDGVNFTAGTFMTGAFP
                     FMMFGLPAAALAMVHEAKPEKKKFVAGIMASAALTSFLTGITEPIEFAFLFVAPVLFA
                     IHCLFSGLSYAVMQLLGVKIGFTFSGGLIDFLLFGVLPNRTPWYLAILVGIGFAVLYY
                     SVFRFMIRKFNLKTPGREDESETNVEVIEMSKNELASEILIALGNKDNLKSLDACITR
                     LRLVVNDINKVDKEKLKSLGAAGVMVIGNNVQAIFGPKSDSIKSDIKDIIEGKTKVES
                     TVTKNVDKNKVKKNMEVANMENALVEKIDSLTNGEILDIEEVPDSVFSSKLMGDGFAI
                     KSSDGLIYSPVDGTIGVIFPTKHAIIIKSKKSGVEILIHLGIETVNLEGNGFEVFVNV
                     GDEVKAGEKLVKMDLEYIEKNGLSTISPVVFTNLEQNQKLSIKKGITTAKEENRVSIL
                     RDGIEKIG"
     gene            complement(8582..9424)
                     /locus_tag="KRL67_001245"
     CDS             complement(8582..9424)
                     /locus_tag="KRL67_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HBH1522141.1"
                     /translation="MIIQQIYNNNVVLVLDENIKKELILTGCGIGFQKKKGQEIDKSK
                     IERTFVIQDESFLDKISKLASQVDEKFFEISTEIIAYAEENLNTKLYEYIYVALTDHI
                     AFAIKRYHENITIKNDLLHEIKRIHKKEYEIGKWAVDYINKEFDVKFPVDEAGFIAMH
                     IVNSNYKGSSKESLLITKIVKDILNIIRYYYRVEFKEDDINYDRLLTHLKFFAKRLVK
                     KEKINDTNNEIIDIIKVKYEKDYDCAYKIKTHVEKNYDYYVSQDELLYLTLHIKRVIS
                     VLNL"
     gene            complement(9685..11727)
                     /locus_tag="KRL67_001246"
     CDS             complement(9685..11727)
                     /locus_tag="KRL67_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HBH1522142.1"
                     /translation="MSILVTLLGLLVCTTTSTVFSKKWSNIPLAIYQIVLGIILSILP
                     FKFSFSFNPEIFVICIIAPLLFSEGQNVSRKELLELRKPILLLAFGLVLITVFAGGIF
                     IHFLIPGMPLSVSFALAAVISPTDLVAVKSITQGLNFPKNMMSILEGESLLNDAAGVV
                     AFKVAVLATVTGVFSIEDAGIQFIITAFGGIIVGSILGYIIIKIRLSLHKWNLEEIPM
                     VIVIQIMTPLFVYFVAEEIGVSGILAVVMAGIAHGIEKEHLQNTTTKLRIISDNTWYV
                     LEYVLNGFVFTLLGFLLPSIYTGLSSKNENMALELTFISTLIVLILFIIRFIWVYLWH
                     NSFIKTKKNPLNNFFVGFLGFKDEEVVKESISKCKYSLIVATCGVHGTFTLATALSIP
                     FYLADKDVFPMRDTVLFISSEVILISLVLATVLLPRLLKNNVQESEPLLSDKEAYKLI
                     LKEAILKLSNEKAQEAQLVIHDLNEQLVDSEKGILNTPDNKLISMMIAEADQQGLLAV
                     LKLLEDGKISDRAFKLYRFYITKSRKSVQKSIFKIVKLKISMWIINRKVKKEVKEKIT
                     KIVSENKEIIKEFHHAYALSTKTAISFINKNTDENNRHEALIAIRFYNRYLNWITRKI
                     QNDDKFEARVEYYRVMAIQSQRDSVQQLVETKQISREVAKELLENILYDEMMMIES"
     gene            complement(11879..12850)
                     /locus_tag="KRL67_001247"
     CDS             complement(11879..12850)
                     /locus_tag="KRL67_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding protein"
                     /protein_id="HBH1522143.1"
                     /translation="MKKEESLLKVEGLKKYFEVKSGLFDKEIKCVKAVDGISFDLKKG
                     ETLAIVGESGCGKSTAGRTILRLIEKTEGKVEFNGVDIYKLEKEELRKLRPKMQMIFQ
                     DPYSSLNPRLNVEQIISEAVIEHNLVPKNEIKDNILNVVESCGLSKYHLNRKPGEFSG
                     GQRQRIGIARALALNPEFIVCDEPVSALDVSIQSQIINLLMDLQDKYKLSYLFISHDL
                     SVVNHIAHRVCVMYLGSIIEIATKGELYLHSQHPYTKALMSAVPIQDPTIKKNRIILK
                     GDIPSPTNPPSGCKFHTRCPYCKEICKKEIPVLKEISRNHFVACHLV"
     gene            complement(12851..13828)
                     /locus_tag="KRL67_001248"
     CDS             complement(12851..13828)
                     /locus_tag="KRL67_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBH1522144.1"
                     /translation="MSKALIEVKDLKVYFHTDKGIVKSVNEVSFNINEGETIGIVGES
                     GCGKSVTAMSLMKLLPTSKIEGGEIIFRGKDILKMNEDELMGIRGNEISMIFQEPMTS
                     LNPAFTIGSQIIEGIMIHQDLSKEEAKKKVIDMIKLVEIPRAEEIYNSYPHELSGGMR
                     QRIMIAMALSCNPKLLIADEPTTALDVTIQAQILDIMKNIKEKLNTSIMMITHDLGVV
                     AEMCDKVLVMYAGKIIEVAEVVELFKNPKHPYTIGLLKSKPVLGKNKDKRLYSIPGQV
                     PNPIGIPDSCYFSDRCEKVCDKCRTQIPPLIELNSGHSIACWLYKKEEI"
     gene            14047..15606
                     /locus_tag="KRL67_001249"
     CDS             14047..15606
                     /locus_tag="KRL67_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBH1522145.1"
                     /translation="MKFKKLASLILVSALMLTFTACASNDKDKSSGGKSGGTVVIPMA
                     SDPDIINGAFANVKEDSLASNIVYAPLYTYEKGNLVNYLAEKVDFKDSKELTIKLKSN
                     LKWHDGKPITAEDVLFTFNTVLDEKQNSPSRQYLLVGEKPVKVEKIDDLTVKITLPTA
                     SESFLYGISKISPIPKHVFEGESNIAKSEKNNNPVGSGPFKFKEWKKGESIVFEKNTD
                     YFGGEPKADSIALKIIPNEASQEAALNNGEISLMKTSAEGYEKAKSNSNLQTYTYSEE
                     RLNYIVFNQNISNMANKEVRQALSYALNRNEMIESAYGKEGSVPAKSILVPEADFYTE
                     EGVEGYDQDTNKAKDLLDKSGIKIDKLKIGYNTGRFGHKNYALVAQQELKKIGIEAEI
                     VPYESKAFFNILFSNSTECDMYVNGYAWGLEPNPYRGMFETGQYCNQTKYSNAEIDAL
                     WEKGFTELNKEKREEIYKQIQQDISKDAPIYTIDYEQNLMAAQKNLKGIKDAKPSPAI
                     LFEDWSKLYVE"
     gene            15712..16671
                     /locus_tag="KRL67_001250"
     CDS             15712..16671
                     /locus_tag="KRL67_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBH1522146.1"
                     /translation="MLKLILNRVKVLIPMLILISILSFGLLELAPGDPADAYINPLMS
                     AQDIENIRVNMGLDKPVYIRYLNWLKNTLNGNLGISYINHMPVTEQIMEKMGNTFILM
                     GTSLIFSILVAIPLGIFLAVNKNSITSKVSSIFNYIGVSIPSFWIGMILISIFSVKLN
                     IFPSGGMHTIGNDSIEDLVKHLVLPVITLGLYNTAIFTNYVEAGVNEQLKKQYVVTAR
                     AKGLSEKVILFKHVLKNSLTSLVTILGMSIQKLVTGAFVTEVVFSWPGMGRLMIDSIF
                     SRDYTVIMAITMLSALFLILGNLVADILYLLIDPKIKSSKGGF"
     gene            16674..17486
                     /locus_tag="KRL67_001251"
     CDS             16674..17486
                     /locus_tag="KRL67_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBH1522147.1"
                     /translation="MFTSILKELKKDKSFAFACITLSIIIILIMASFILNIDSNAIDT
                     TSKLQEPSLKHIFGTDELGRDYFSRALYGGRISLSVGILSMLISVVFGTAVGVISGYI
                     GGKLDQFLMRLVDMLMSIPSFLVLIVLNTYLTPKVSTIILIIGFLSWMEVSRIVRGET
                     LKIKENEYCLAAKATGIKTRNILLRHIVLNLKETIVVAGSLNIANAILTESALSFLGL
                     GIQLPLASWGNMLQDAQRHIFDKLYLAVFPGLLIFLTVMSLTIISKKFYSNT"
     gene            complement(17703..18605)
                     /locus_tag="KRL67_001252"
     CDS             complement(17703..18605)
                     /locus_tag="KRL67_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBH1522148.1"
                     /translation="MDIKQLKYFVEIVKSGFNLSIASKTLHISQPALSQIIKNFEENE
                     DVYLFERYKGRLNGLTPAGERFYVNAEHIINEYKNMMEDLREDSVQFKGKVRIGIPPL
                     ILGIAFSDVVAQLVANNPDIEFDIVEKGAYDLNRMLILQELDYAVLLHPHKIDKSVVT
                     EHVLQEDELTAFLNINHPLAKKDKIDWNDLNEQLLAIFNPTFMIHHKLMQKFTDENVK
                     LKRYIMSGSWDFLLLSTTNSDFITILPSPVHDFFENSKIIEKPFNHPITWKVILCRPK
                     KERYSHVDQHVFKFIIDFFSKKNS"
     gene            18960..20138
                     /locus_tag="KRL67_001253"
     CDS             18960..20138
                     /locus_tag="KRL67_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000786547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HBH1522149.1"
                     /translation="MKEVVIVSAVRTPIGSFGGVFKNTSAVQLGTIAVKEAISRVGLN
                     LSEIDEVIIGNVLQTGLGQNVARQIAINAGIPNSVPSYTVNKLCGSGLKSVQLAAQSI
                     TSGENDVVIAGGTENMSQAPYIVPTARFGSKMGDITMVDSMLTDGLIDAFNQYHMGIT
                     AENIATKFEFTREMQDKLALESQNKAENAIKNNRFKEEIVPVDVLIRRGKIETIDKDE
                     YPKLGMTFEGLAKLKPAFKKDGTVTAGNASGINDGAAMLILMSQQKADELGIRPLAKI
                     KSYASAGVEPEVMGTGPVPATRKALKKAGLSINDIDLIEANEAFAAQALAVKNELQID
                     SSKLNVNGGAIALGHPIGASGARILVTLIYEMQKRKVETGLATLCIGGGQGISMVVSR
                     "
     gene            20147..20803
                     /locus_tag="KRL67_001254"
     CDS             20147..20803
                     /locus_tag="KRL67_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase subunit A"
                     /protein_id="HBH1522150.1"
                     /translation="MNKIVSIDEALSHVKDGDTIMVGGFMANGSPENLIDYLCSKNIK
                     DLTLICNDTGFIDKGVGKMVVNKQFKKIIASHVGLNKETGRQMNNNETDVELVPQGTL
                     AEQIRAAGYGLGGILTPTGIGTLIEEGKQKIVVDSKEYLLEKPIYADVALLFASKVDK
                     AGNLVYKGSMNNFNNLMASAAKITIVEADEIVEIGDIDPNEVNTPGIFVNYIVEGGNL
                     "
     gene            20803..21468
                     /locus_tag="KRL67_001255"
     CDS             20803..21468
                     /locus_tag="KRL67_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacid CoA-transferase subunit B"
                     /protein_id="HBH1522151.1"
                     /translation="MDKLEMQEYIANRVSKELKDGAVVNLGIGLPTKVANYIPDDVNV
                     ILQSENGFLGLGSAEDGNSSDETIVNAGGQPVTILPGGCFFDSATSFGIIRGGHVDIT
                     VLGALQVDKYGNIANYMIPGKMVPGMGGAMDLVTGAKKVIVAMEHTSKGSAKILNNCT
                     LPLTATNAVDLIVTEMGVMEVTSDGILLKEINPAFTLDDVISATEAPLILSDSLNGNI
                     SVV"
     gene            21573..22349
                     /locus_tag="KRL67_001256"
     CDS             21573..22349
                     /locus_tag="KRL67_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxybutyrate dehydrogenase"
                     /protein_id="HBH1522152.1"
                     /translation="MVKDKVVFVTGAASGIGKQIAESFLKNGAKVMFSDINQEALNNT
                     IAELQKEGYDCMSVKCDVTKEEEINHAIDKTVEKYGRLDILINNAGLQHVSMIEDFPT
                     DKFEFMIKIMLTAPFIATKKAFPIMKKQGFGRIINMASINGVIGFAGKAAYNSAKHGL
                     IGLTKVAALEAADSGITVNALCPGYVDTPLVRGQFNDLSKTRNIPLENVLEEVLYPLV
                     PQKRLLDVQEIADYAMFLASDNAKGITGQACLLDGGYTAQ"
     gene            22411..23745
                     /locus_tag="KRL67_001257"
     CDS             22411..23745
                     /locus_tag="KRL67_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntP family permease"
                     /protein_id="HBH1522153.1"
                     /translation="MSIIAPIGIIIGIIAIIYFSVKEIHITIAAPIATLIVVLLNKMD
                     VVTSMLGADKNNYMGALGNYIMSYFAIFLLGSILAKFMEESGATVSIADFILNKFGSN
                     HPYRVLVAIFIVSFILTYGGISLFVVMFAVIPLARTLFKKLDISWNLIQIPLWLGIAT
                     ITMTIIPGTPAIQNVIPIQYLNTSLTAATIPSIVGSIGCATFGLVYMKYALNKSIKKG
                     ETYATYTSELEEESLDRELPGFFASIFPLLALVIIAITGSTFGSEFMKKNVIYIALIV
                     AILLSVFLFHKFLSSKIQTLSIGASGSIGPIFATSSAVAFGAVIMAAPGFDVFSKLIM
                     SIPGSPLISLTVLTSTMSAITGSSSGALGIVMPNFADYYLSTGLNPELIHRVATIASN
                     ILTIVPQSGVFLTFLSLSKLTHKTGFKETFIVVAVGSLIAEIIVITLGLLMY"
     gene            24242..25168
                     /locus_tag="KRL67_001258"
     CDS             24242..25168
                     /locus_tag="KRL67_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation protein"
                     /protein_id="HBH1522154.1"
                     /translation="MMRKNIPTKLKTILALGVLTLATASSAVTASASSLENNVNISNK
                     NLVYSNTKYKFSAKPNCSVKPELKPDKDNSCKDKNHNDKNCNNTNKPEDNNNSDSTNK
                     PDNNKPESNKPEDNNNSDSTNKPDNNKPESNKPEDNNNSDSTDKPDNNKPESNKPEDN
                     NNSDSTNKPDDNNNSGSTSENFSAYQKEVVDLVNVERAKAGLNPLTLDSSISNVATKK
                     SQDMIDNNYFSHNSPTYGSPFDMLKKFGISYKTAGENIAMGQKTPKEVVNAWMNSEGH
                     RKNIMNPNFSKIGVGVAQKSGGSIYWTQIFVG"
     gene            complement(25562..29101)
                     /gene="nifJ"
                     /locus_tag="KRL67_001259"
     CDS             complement(25562..29101)
                     /gene="nifJ"
                     /locus_tag="KRL67_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009900447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HBH1522155.1"
                     /translation="MAKFMKTLDGNTAAAHVAYAFTDVAAIYPITPSSTMAEVVDEWA
                     SQGRKNIFGQTVNVVEMQSEAGASGTFHGSLQGGALTSTFTASQGLLLMIPNMYKVAG
                     ELLPGVFHVSARALASQALSIFGDHQDVMAARQTGCVLLASGSVQEVADIAPVAHLAA
                     IEGRLPFIHFFDGFRTSHEIQKVELLENEDYASLLNFEAVQAFRDNALSPNHPVARGT
                     AQNPDIYFQTREASNKYYQNIVGIVEKYMEKMSNLTGRKHSLFDYYGAEDAKYVLIAM
                     GSVTETIEETIDYLNAKGEKYGLVKVHLYRPFSMKHFLDAMPSTVERICVLDRTKEPG
                     STGEPLYLDVRDVFYGKENAPMIIGGRYGLGSKDTTPSDIKTVFDNLVSEQPKNGFTV
                     GIVDDVTNTSLTPSEPIKIASKGTIRCKFWGLGSDGTVGANKQAIKIIGDHTEKYAQA
                     YFDYDSKKSGGITMSHLRFGDTPIRATYLIDEADYIACHKQSYVYQYDLLKGLKKGGT
                     FVLNTIWDQAGLEEHLPAHMKQYIAKNDIKFYTVNAVKLGQEIGLGNRINMIMQSAFF
                     KLAEIIPEEDAVKYLKDSIVKAYGKKGEKIVNMNYAAVDAGINALVKVEVPASWENAV
                     DADKGEVKEPEFIKNILRPMTAQEGNNLPVSTFNGIEDGTFPCGTAAYEKRGIAVDVP
                     EWIIDNCIQCNQCSFICPHACIRPVLVTEEELANAPEGFEAKKALGKGLEGLKYRIQV
                     SPLDCTGCGNCADICPAKEKALVMKPIDTQLDTELDNWAFAVNPDEVAPKVDVMPANT
                     VKGSQFRQPLMEFSGACAGCGETAYIKVVTQLYGDRMMIANATGCSSIWGASAPSIPY
                     TCNHEGKGPSWANSLFEDNAEYGFGMYTAVKQIRNKIVDAMTELVSMDICEDAKAVFT
                     EWLDSRNDGEASKVASAKVVELLEKPACDCTDEKAKELVKAIKDRKDYLVKRSQWILG
                     GDGWAYDIGYGGLDHVLASGEDVNVLVFDTEVYSNTGGQASKATPAAAMAKFAASGKK
                     SRKKDLGMMAMTYGNVYVAQVAMGADKNQFIKAVLEAEKHDGPSLIIAYAPCINHGLK
                     EGMGRTVANEAQAVACGYWHLYRFNPEVKAEGKNPFTLDSKEPTASFKDFILAQVRYS
                     AIAKQFPEEADGLFAKAEADAKERYEGYKKLAE"
BASE COUNT        10150 a         4690 c         3793 g        10707 t
ORIGIN      
        1 ataaaattat atttctaaaa taggtataac atacctatag aaattactat tgttattatt
       61 gaaagtattt tactataatt ccaaatacgt tttccaccaa aacattcaaa tggataaaaa
      121 ggtatactat gtaaaattaa taaacttttt ccaagtgaaa ctatgtattc acaaaattct
      181 gattgtcttg caaatatctc tatcaacaaa ataagacaca gtaatcctaa ccattcaaat
      241 aatccttcaa tagccatact tcgtttaaat atatcagttg acttatattc ttttggataa
      301 aatcgaccta tcattggcca tataccacct aatactgtaa tcaacaatga tataaacaac
      361 cctcctcttg taacagtaaa tacccatttt tcaggtaaaa ttaaagttgc aagtagacca
      421 ctaattagag aaataatcaa caaagaaaaa aatgcaccta ctataatggt aaaatcattc
      481 agattattat caaataataa aataggaaac aatatcaata agtttaaaat caaataaact
      541 cctaattgtt taattcatga gttactcttt tgtgctgatg tactccacag atggaatcca
      601 gaagcaataa aagctttttt tggctttcta aatgccaaac tctctaccaa tccaaaactt
      661 gatttaccaa gttcccacac aaaccgtctt tcatcatcca ccatttctct aattattata
      721 ttatccaaat tatccctccc aagttcttaa ataattttta atatacttaa ttgcataaac
      781 tctacaatta aataaatgtt tctatcaaaa cacttcatat actttaaaca aatatcatag
      841 tatttctata acaatttata ttcttaatag tagtatattt ttataaaaaa aaagtgagca
      901 ctgattaaac aaggttcact ttttttaatt tttatattgt atttcataaa tttatttaat
      961 ttgtttattt tccctttaca ctatatctat ctaatattaa tttttcatat tcttctattg
     1021 gcattggtct agcaaataaa aatccttgag ccatatcaca ccctataaac cttaaaaatt
     1081 ctgcttgctc cacagtctca acaccttctg aaacaacttt tatatcaagt tgctttgcca
     1141 tttgaacaat acttgaaact acgatttgac tcttttttgc atctcctact gttataaaaa
     1201 attgtctatc caatttcaat acatcaattg gcatatcttt aagcatattt aaagaagaat
     1261 aaccagaacc aaaatcatcc atagaaattt taaatccaac agattttaaa gtctgcatta
     1321 ttttaaataa tatttttgta ttatcaaaga ttgcactttc tgtcaattca atttcaatta
     1381 aatttggctc aatttcatat tcttttgtta ttctttcaag ttcagatata aaatctggat
     1441 tctcaagatg tacccttgat aagtttattg atataggaaa tgtagggaaa ctttcatttt
     1501 cccaacgttt aaaattttta caaacttgag taaatacaaa catatccaat tctgttataa
     1561 aaccattctt ctcaaaaata ggaataaata caccggggct tattagtccc tttttaggat
     1621 gttgccaacg tacaagggct tcagcaccta caattttagt agttgacaac tcgattttag
     1681 gttgtaagta aactttaaat tgttcatttt ttaaggcatc atgcatttct tgttcaatat
     1741 cacgttcttc tatgagagtt tcttttaaat tttcagcata aatctcataa acttttaaat
     1801 attttctttt tactgtagtt tttgcaatca tagctttatc aagacaagta gaaatatttg
     1861 tttctccatc ttcaacgaaa tatacaccta tggatggaat taaattgagt tctactccca
     1921 aacttgtttt tatcattgac aattttttac gtataagttc acatatctct acaactgact
     1981 ccctatcatt tttcttttca aatattatag caaaattgtc atttgaaatt ctagaaaata
     2041 cagattgctc tttaaatata ctttttacta tctctgctat cccttttaat actctatctc
     2101 cttcttcata cccaaaccta tcatttacta gcttaaactt atctatatcg aaataaatta
     2161 aaactagatt tcttttctca tgagaggata aaaactttcc tccctcaaga ttaaatttat
     2221 ttgagttgcc aatgcctgta acgttatcct taaaagcaaa gtcttctaat ttttccttac
     2281 tctttttctg tataaataat atatacattg ttaatgccgt aaatattata atcaagacta
     2341 cacatgctga taatgtaagt tttataatag catatgattt ctcagatata gcagttttag
     2401 gaacttcaga aaaaagatac caatcattga ttcctatggg ggcataactt aaataatatc
     2461 catttccaga catattataa cttaatgaac ccgatttaaa gttttttata tttgatttca
     2521 tattattaag agtagatttt gatattgaat cacttctgcc aatgtaagaa aataaatttt
     2581 ctttagtaga gttggcgttt ttactgcttg gatttaaaac aatatcacca ttacttctaa
     2641 ctatataaga atatcctttt ccattaaatg taggtacaga aagtattttt tcatacaact
     2701 tcgtactatg tgtagcaaat ataactcctt taacctcatt atctttataa attggtacag
     2761 aatatacatt aatattacta ttggtatttt tatcatcttt tattacatct tttaattttc
     2821 ctgttataac cacttctcct tgcattgatt ttaagaaata atctctttta gagaagtctt
     2881 ctatataacc atctgtagtg tatgctgtac catttggtaa aataactccc attcttttaa
     2941 atgaactatt aatagcttgt ctttttagta tagaaaggat attatctact tcaaaagact
     3001 cttctccttc aataaatatt gaaattgact ctagtaactt tatatctcca ttaatttgtt
     3061 tttttaacac attgacgctt tgatttgcta tttctgaaag atattgattc gtttcttcac
     3121 ctaatgtctt atttaacctt gaaccataaa tcaaacagga aattgtaatt actaagatac
     3181 ttatgacagc aattatggac aaaccatacc tattattttt tgcatcactt aacattatgc
     3241 ttcctgcctt tctcgaatct atgtatgtat aatcattatc ttatgacaat tattgtaaac
     3301 attttataat aatacttatt tttccatatt tactgtaatt atacaaaatt ataaacttta
     3361 ttacaacaat aaattaatta atatagatat tagggtttat attattgcat tctctatttt
     3421 acaatcattt acatataaaa aaatgtaata tcaaagtgaa acttgatatt acatttttgt
     3481 tattgtattt tgtttacttt ttatactaca ttttacttaa acactcattt gcaacttctt
     3541 tatcatcaaa atgatgctta actttaccta taatttgata agtttcatgt cccttacctg
     3601 ttataattac aacatcatcc ttttttgcca tacttatagc ttctgctata gcttgttttc
     3661 tatcaacaac aacatggtaa ttttcttttt tcttgtcaat tccttcaagt atatctttta
     3721 ttattaactc tggttcttca gttcttggat tatcagaggt tataatacat aaatcagaat
     3781 atttttgtcc aattgatccc atcaaaggtc tcttttcagt atctctatct ccaccacatc
     3841 caaatacaga aattattttt ccttctgcaa attgttgagt tgtctttagt acattttcaa
     3901 gtgcgtcagg agtatgagca tagtctacaa ttacacttat acctttatca tttggaactg
     3961 tttcaaatct tcctgatact ccattagaaa gtctaagacc ttctttatat attggtttag
     4021 gtatgccaag tacatagcaa gcagctataa ctgctaaagt attatatact gtaaacattc
     4081 ctggaactgg tataaatatt acttcttcat atgaaggtgt tattaatcta taagatactc
     4141 catccgaatc tgattttata tctctagcca taaaatcagc cttagtatca acaccataag
     4201 tgtaacaagg tacatttata ccttttatat tttcatatat cttttttcca ccttcatcat
     4261 ctatgtttat tatatttgcc atagtagtct taaaaaataa tttttctttt gcttttctat
     4321 aatcatctaa atctttatga aaatctaaat ggtctggtgt taaatttgta aatatgccta
     4381 gtttaaagtc tgtttcgtca actctattta atgccaatga atgtgatgaa acttccatcg
     4441 cacagtagtc acatccatta tccaacatat tattaaaatg atattgtaaa tctatacttt
     4501 ctggagttgt tgaattggaa acaatgtctt tttctccgtc aaaaattttt atagttccta
     4561 taagtccaac tttattctta tttaaagtta atatctcatt tagtatagta gttatactag
     4621 tttttccatt agtacctgta actcctataa cgttaaattt ttgagatgga tgattgtaaa
     4681 aattatcagc tacttttgcc atatcttttc ttgtatcttt tacctttata aatgtacatc
     4741 cttttatttc tacatcttct tcaacaagaa atgcacttgc tcccttttct atggcatctt
     4801 ttatatactt atgaccatct gacacaaatc cttttataca tataaataat gtaccttttg
     4861 taacctttct tgaatcatat tgtacattat taatatctat atttaattca cccttgacat
     4921 tgattatatc caacccctgt ataatatcat ttaatttcat aaatgctttc ctccctttaa
     4981 actctttctg ctaaaattac actacatata atattatatt aagaattttt tactatgtga
     5041 atggttaaaa atgcttgaaa cattgcaaaa aatgctctat aattttctat aatataactg
     5101 aggaaaggtc ttctataaaa actatggata ataaaatcgg atacttaaat gataacttta
     5161 aaatatttaa tattagagat aaaaaagata tacaatttga ataccataat catgatttta
     5221 ataagataat tatatttata aatggaaatg taacttattt tgttgatggt attccttata
     5281 aattaaaacc ctgggatata ctttttatca gcaacaatga aatccataaa ccagttatag
     5341 attcaaatgt attttatgaa agaattgcaa tatggattag tccagaattt atgaaaaaaa
     5401 atagtgatgc taatagtgac ttagaaaaat gttttaaaat agctacagaa aataaatgta
     5461 atagattgcg tttaaatata catgatattg aatctattaa gagttttatt gagcaaataa
     5521 atatttccga atccaatact gaatttggaa atgaaatatt gagaaacgca ttatttatac
     5581 aacttatgat attcttaaac agaattttcc taaaaaatga aaatatggaa tcaacagaag
     5641 acgtctttta tgatgaaact attaagaaag tattaaacta tataaattca aatatagatg
     5701 ataagctttc aatagatagt atatcgtctg aattttttat aagtaagtat tatcttatga
     5761 gaaaatttaa atctcatact ggaacatcta tacacaatta tatagttcaa aaaagattaa
     5821 ttttggcaaa gtcactaata accaatggtc accttatgag tgaagtttgt aataaatgtg
     5881 gattcaacga ttattctagt tttgtaaggg catttaaaaa aaactacggt gtatctccta
     5941 aaaattatat aaacaataaa aatcctttag atattactct tttagatgaa tagatacttt
     6001 atgtaaattt tattttaata aaaacttgaa gtattagtat atttatctta ctaatacctc
     6061 aagtttttta atgttttaaa atatatcaaa atataatgat atctatttat attacttaaa
     6121 atatatatta aataaattgc atgttaactg aacctattta tatatttatt aaatctcaca
     6181 taacttttag ttatactatc atatagctat cctatttttt caataccatc tcttaatata
     6241 cttactctat tctcttcttt tgctgtagtt attccttttt taatgcttaa tttctgattt
     6301 tgttctaaat tcgtaaatac cacaggactt attgttgata gaccattttt ttcaatatat
     6361 tctaagtcca ttttaactaa tttttctcct gcttttactt catctcctac attcacaaat
     6421 acttcaaatc catttccttc taaatttaca gtttctatcc ctaaatgtat aagtatttct
     6481 actccacttt ttttggattt tattattatt gcatgcttag ttggaaatat aactccaata
     6541 gttccatcaa ctggtgaata aatcaatcca tcactagatt taattgcaaa tccatccccc
     6601 atcagtttac ttgaaaatac agaatctggt acttcctcta tatctaatat ctctccattt
     6661 gttaatgaat caatcttttc tactaaagca ttttccatat tggcaacttc catatttttt
     6721 ttcactttat ttttatcaac atttttagta acagtacttt caacttttgt ttttccctct
     6781 attatatctt ttatatcaga ctttattgaa tccgattttg gtccaaatat agcttgaaca
     6841 ttgttgccaa taaccataac acctgctgct ccaagtgatt tcaatttttc tttatctact
     6901 ttatttatat cgtttacaac caatctaagt cttgttatac atgcatctag actctttaag
     6961 ttgtctttat ttcctaaagc aatcaaaatc tctgatgcta actcattctt agacatttca
     7021 atgacttcaa cattagtttc tgactcatct tctcttcctg gtgtctttaa attaaatttt
     7081 cttatcataa atctaaatac gctatagtat aatactgcaa aaccaatacc taccaaaata
     7141 gccaagtacc atggagttct attaggaaga acaccaaata ataagaaatc tattaatcct
     7201 cctgaaaatg tgaatccaat tttaactcct aataattgca taactgcgta tgataaacct
     7261 gaaaataaac aatgtattgc aaataatact ggtgcaacaa ataaaaacgc aaactctatt
     7321 ggctcggtta ttcctgttaa aaaagatgtt aatgctgctg atgccattat acctgctaca
     7381 aattttttct tttctggctt tgcctcatgc accatagcaa gagctgctgc tggaagacca
     7441 aacatcataa atggaaatgc acctgtcata aaagtacctg ctgtgaaatt tactccatct
     7501 ttaagttgag caaagaatat cttttggtca cccattacaa gctcaccagc tttatttaca
     7561 tattctccaa attgatacca aaatggatta tggaaaacat gatgcaaacc aaatggtatt
     7621 aatagtcttt ccaaagaacc ttccaaaaat gctgcgagtg tttgatttgc ttctataaca
     7681 ttttttgaga aactaagtaa tccctcttgt attggaggcc aaataaaaga caatataatt
     7741 cctattgcta tagcaactaa tgaagtaatg attggtacaa atcttttacc tgcaaaaaaa
     7801 cctagatact gaggtaattc aattttataa aactttttat aaagataagc agccactatc
     7861 cctataataa taccaccaaa tacccctgtt tgaattgttg gtattccaac aatacttgca
     7921 tacatagaat tatctatcat atcttctgta acacctgcca tgacacccat agatgtattg
     7981 agaattaaaa aacctactat ggctgccaat gctgcaacac cttctccttc agttaaacca
     8041 acagctaccc caactgcaaa tataagtggt aagttattaa atataatttg acctgaactt
     8101 tccattatac ttattataag ttgaaatgta ggattcgcca aagctggaaa atgagccaac
     8161 aatgcatctc ctgctaaagc attactcacg ccaagtaata tacctgctgc aggaagtaat
     8221 gcaactggta gcattagaga ttttcctact ttttgtaaaa caccaaatac ttttttcatt
     8281 tacttttctc tccttattat tttatatttt tttaaataaa aaagacttaa gtttataaaa
     8341 agttacatta gtatacttaa ttctaagatt aaacttatgt taatataaat aaacctatat
     8401 taacctaatt tcagaattaa aaataaacta ttataatttt tttataaaca taagtcttgc
     8461 ctgcattatc agtaacaatc ttaatgtttt aaaattatta ttttatatta aattttaaat
     8521 aatatattaa aattaaacat aatatattaa atctatacac tctgtattaa actgctcttt
     8581 atcataaatt cagtacagat ataacccttt tgatatgtaa tgttaaataa agtagttcat
     8641 cttgacttac atagtaatca taattctttt ctacatgagt ttttatttta tatgcacaat
     8701 cataatcttt ttcatatttt actttaatta tatctataat ttcattattt gtatcattga
     8761 ttttttcttt ttttactaat cgttttgcaa agaattttaa atgagtcaga agcctatcat
     8821 aatttatatc atcttcttta aattcaactc tataataata tcttataata tttaatatgt
     8881 cttttacaat tttagtaatt aacaatgatt cctttgaact tcctttatag tttgaattta
     8941 ctatatgcat tgctataaaa ccagcttcat ccactggaaa ttttacatca aattctttat
     9001 ttatataatc tacagcccat ttcccaatct catattcttt tttatgtatt ctttttatct
     9061 catgtagtaa atcattcttt atagttatat tttcatgata tcttttaatt gcaaatgcaa
     9121 tatggtcagt aagtgctaca taaatatatt cataaagctt agtattcaaa ttctcctctg
     9181 cataagcaat tatttcagta ctaatctcaa aaaatttctc atcaacctgg cttgctagtt
     9241 tactaatttt atctaaaaag ctttcatctt gtattacaaa agttctttca attttacttt
     9301 tatctatttc ttgacctttc tttttttgaa acccaattcc acatcctgtt aatattaatt
     9361 ctttttttat attttcatct aaaactaata caacattatt attataaatt tgttgaataa
     9421 tcataatcaa tatctctccc aaaatacaat ttcccttatt cataaaaaaa tacctaaatt
     9481 aagatacaat tcacctgtat accttaacct aggtattgcc tgcttaccag taacaatcca
     9541 gcttgttaac gcttatatat tgtattcaat ttatggtttt attctattat aaataaatat
     9601 atatgtcaag taaattattg aaaactattt cctatttttt agtattaatt ttaatatcaa
     9661 aaaacaaatc ttattccatt atatttatga ttctatcatc atcatttcat cataaagaat
     9721 attttccaat aactctttag ccacttctct tgatatttgc ttagtttcaa ctaattgttg
     9781 aacactgtct ctttgacttt gtatcgccat tactctgtag tattctactc ttgcctcaaa
     9841 cttatcatca ttttgaatct ttctagtaat ccaattcaaa taacgattat agaatctgat
     9901 tgctattaag gcctcatgtc tgttattttc atcagtattt ttatttataa atgatatagc
     9961 agttttagta gaaagtgcat atgcatggtg aaattctttg attatttctt tattctcaga
    10021 tactattttt gtaatttttt cttttacttc tttctttact tttctattta taatccacat
    10081 gcttattttt aattttacaa ttttaaatat agatttttga acagattttc ttgattttgt
    10141 aatataaaat ctatacaatt taaatgctct atcagaaatt ttaccatctt ctaatagttt
    10201 caaaacagct aataatccct gttggtccgc ttctgcaatc atcatactta taagtttgtt
    10261 atctggagta tttagtattc ctttttctga gtctaccaac tgttcattta aatcatgtat
    10321 aactagttgt gcttcttgtg ctttttcatt actaagtttt aaaatggctt ctttcaaaat
    10381 tagtttatat gcctctttat cagataataa aggttctgac tcttgtacgt tattttttaa
    10441 taatcttggc agtaaaactg ttgccaatac taagcttata agaataactt ctgatgatat
    10501 aaataaaaca gtgtctctca ttggaaatac atctttatca gccaaataaa atggtataga
    10561 taaggcagtt gctagtgtaa atgtaccatg aacaccacaa gtagcaacta ttagtgagta
    10621 tttacactta gaaatactct cttttacaac ttcttcatcc ttaaaaccta aaaaacctac
    10681 aaaaaaatta tttaaaggat tctttttagt ttttataaaa gaattatgcc ataaatatac
    10741 ccatataaat cttataataa ataaaataag tacaattaaa gtagaaataa aagtcaattc
    10801 caatgccata ttttcatttt tactactaag tcctgtatat atacttggta acaaaaaacc
    10861 aagcagtgta aagacaaatc cattcaatac atactccaaa acataccaag tattgtctga
    10921 tattatcctt aatttagtag ttgtattttg taaatgctct ttttcaattc catgagctat
    10981 ccctgccatt acaacagcta ggattccaga tactcctatt tcttctgcta caaaatatac
    11041 aaaaagtgga gtcattattt gtataactat taccattgga atttcttcca aattccactt
    11101 gtgtaaacta agtcttattt ttataattat gtatcctaaa atacttccta ctattattcc
    11161 tccaaaagct gtaattataa attgaatccc tgcatcttct attgaaaaaa ctcctgtaac
    11221 tgtggcaagt actgcaacct taaatgctac tactccagca gcatcattta ataaagattc
    11281 accttcaagt atactcatca tatttttagg aaaatttaat ccttgtgtaa ttgattttac
    11341 tgctactaaa tcagttggag atattactgc tgccaaagca aatgaaacag ataatggcat
    11401 cccaggaata agaaaatgta taaagattcc acctgcaaac acggttatta aaactaaacc
    11461 aaatgcaaga agcaaaattg gttttctaag ctctaatagc tcttttctcg atacattttg
    11521 tccttcactg aatagtaatg gagctatgat acatattaca aatatttctg gattaaatga
    11581 aaatgagaat ttaaatggca aaatagacaa tataatccct agtacaattt gatatattgc
    11641 aagaggaata ttactccact ttttgctaaa tacagttgaa gtcgttgtac aaaccaataa
    11701 tcccaacaat gttactaaaa ttgacataac tacctctctt ttcaataata atattataga
    11761 ttaaacttat atatcaacta acttaatatt aacagcatga aatcccatct taatcaaaaa
    11821 aattttcagt ttattctatt attcttatta tatacatcaa atttaatttt tgaaatatct
    11881 atactagatg acatgcaaca aaatgatttc ttgatatttc ttttaaaact ggaatctctt
    11941 ttttgcatat ttccttacaa taagggcatc ttgtatgaaa cttacatcca cttggtggat
    12001 ttgttggaga tggtatgtct ccttttaata taattctatt tttcttaatt gtagggtctt
    12061 gtattggtac tgcgctcata agtgcttttg tatacggatg ttgagaatgt aagtaaagtt
    12121 ctcctttagt tgcaatttct attatagaac ctagatacat aacacaaact ctatgtgcaa
    12181 tatgatttac tacactcaaa tcatgagata taaataaata tgaaagttta tatttatcct
    12241 gtaaatccat aagtaaattt ataatttgag attgaataga aacatctagt gcagaaacag
    12301 gttcatcaca aactataaat tctggattta atgctaatgc tcttgctata cctattctct
    12361 gtctttgacc tccagaaaac tcacctggtt ttctatttaa atggtactta gaaagaccac
    12421 aactttctac aacatttaat atattatctt ttatttcatt ctttggcact aaattatgtt
    12481 ctataactgc ctcggatatt atttgttcca catttaatct tggatttaat gaactatatg
    12541 ggtcttgaaa tatcatttgc attttgggtc ttaactttct aagttcttcc ttctctaact
    12601 tatatatatc tacaccatta aattcaactt taccttcagt tttttcaatt aatctaagaa
    12661 tggttcttcc tgcagttgat tttccacatc ccgattctcc aactatagcc aaagtttctc
    12721 ctttttttaa atcaaaagaa attccatcta ctgcctttac acatttaatt tccttatcaa
    12781 ataatccaga tttaacttca aagtactttt taagaccttc tacttttaat aaactctctt
    12841 cttttttcaa ttatatctcc tcctttttat ataaccaaca tgcaatacta tgaccactat
    12901 tcaattctat taaaggaggt atctgagttc tacatttatc acatactttt tcacatctat
    12961 cactaaaata acatgaatct ggtattccaa ttggatttgg tacttgtcct ggaatcgaat
    13021 acaatctttt atctttgttt tttccaagaa caggctttga ttttagaagt ccaatagtat
    13081 aaggatgttt tggattttta aataattcaa caacctctgc cacttctata attttaccag
    13141 catacatcac taatactttg tcacacattt ctgcaactac tcccaaatca tgtgtgatca
    13201 tcattataga tgtgtttaat ttttctttta tatttttcat tatatctaaa atctgagctt
    13261 gaatagtaac atctaaagct gttgttggtt catcagctat aagtaatttt ggattacatg
    13321 ataaagccat agctatcatt attcgttgtc tcataccacc tgataattca tgtggatatg
    13381 aattatatat ttcttcagct cttggtattt ctaccagttt tatcatatcg attacctttt
    13441 tcttagcttc ttcctttgat aaatcttgat gtatcataat accttcaatg atttgacttc
    13501 caatagtaaa tgctgggttt aaagatgtca ttggttcttg aaaaatcatt gatatctcat
    13561 tccctcttat tcccataagt tcatcttcat tcatctttaa aatgtctttg cctcgaaaga
    13621 ttatttctcc accttctatt tttgatgttg gaagtaactt catcaaagac atggctgtta
    13681 cagatttacc acaccctgat tctccaacta ttccaatagt ttcaccttca tttatattaa
    13741 aacttacttc atttacactc tttacaatcc ctttatctgt atgaaaataa acttttaaat
    13801 ctttaacctc aattagtgct ttactcatta ttttctcctc tcagcaaaat tacttaaaat
    13861 aacatacacc ttttgattgt attttataaa aatttaaatt acaacatttt cttacatttt
    13921 attatttttt tctattaatt ataataatat aatagttaat ttctatatat attgatttaa
    13981 taatgattac tattatacaa tgaaaaatat gtgttaaata ttattttatg gaggttttat
    14041 aagaatatga aatttaaaaa attggcatca ttaatcttag tttcagcatt aatgcttaca
    14101 tttacagcct gtgcttctaa tgataaagac aagtctagtg gaggcaaatc tggtggaact
    14161 gttgtaatac ctatggcaag tgacccagat ataatcaatg gagcttttgc aaatgttaaa
    14221 gaagactctc ttgcatcaaa tatagtttat gctcctcttt atacttatga aaaagggaat
    14281 ttagtaaatt atttagcaga aaaagtagat tttaaagata gtaaggaatt aactataaaa
    14341 ttaaaatcca atttaaaatg gcatgatgga aaaccaataa ctgctgaaga tgttttattt
    14401 acatttaata cagttttaga cgaaaaacaa aattcaccat caagacaata cttattggtg
    14461 ggtgagaaac cagttaaagt agaaaaaatt gatgatttaa cagttaagat tactttacca
    14521 actgcaagtg aatctttttt atatggtata tctaaaattt ctccaatacc aaaacatgtg
    14581 tttgaaggag aatcaaatat agctaagtca gaaaaaaata ataatccagt tggttctggt
    14641 ccattcaagt ttaaagaatg gaaaaaaggt gaaagcatag tatttgaaaa gaatacagat
    14701 tattttggtg gagaacctaa ggcagattct atagcactaa aaataatccc taatgaagca
    14761 tctcaagaag cagctttaaa taatggtgag atttcactta tgaagacatc tgctgaagga
    14821 tatgaaaagg caaaatcaaa ctcaaactta caaacttata cttatagtga agaaagatta
    14881 aactacattg tatttaacca aaatatatca aatatggcta acaaagaagt aagacaagcc
    14941 ttatcatatg ccttaaatag aaatgaaatg atagaatcag catatggaaa agaaggttct
    15001 gtacctgcta aatctatatt agttccagaa gcagacttct atacagaaga aggtgttgaa
    15061 ggatatgacc aagatacaaa taaggctaaa gacttattgg ataaaagtgg tattaaaatt
    15121 gataaattaa aaataggtta taacacaggt agatttgggc ataaaaacta tgcattagta
    15181 gctcaacaag agttgaaaaa aattggtata gaagctgaaa tagttcctta tgaaagtaaa
    15241 gcattcttca atatactatt tagtaatagc actgaatgtg atatgtatgt aaatggttat
    15301 gcttggggat tagaaccaaa cccatataga ggtatgtttg aaactggtca atattgcaac
    15361 caaactaaat attctaatgc tgaaatagat gctttatggg aaaaaggatt tactgagtta
    15421 aacaaggaaa aacgtgaaga aatatataag caaatacaac aggatatatc taaggatgca
    15481 cctatttaca ctatagatta tgaacaaaat ttaatggcag ctcaaaaaaa tctaaaagga
    15541 ataaaagatg ctaaaccatc tcctgcaata ctatttgaag attggtctaa actgtatgta
    15601 gaataattga tataaaattt tatattatta attagaaaag gagatgtcta aaaataatat
    15661 tgttttatta ttttaaaatg agacatctct tttaattagg agaatttatt tatgttaaaa
    15721 ttaatattaa atagagtaaa agttttaata cctatgttaa tcttaatatc tatattatca
    15781 tttggacttt tagaattagc tcctggtgac ccagcagatg catatataaa tcctttaatg
    15841 tctgcacagg atattgaaaa tataagagtt aatatggggc ttgataaacc agtgtatata
    15901 agatatttaa actggttgaa aaacacttta aatggaaact taggcatttc ttatataaac
    15961 catatgcccg tgacagaaca gattatggaa aagatgggaa atacatttat tcttatgggt
    16021 acttctttaa tattttcaat attggtagct atacctcttg gaatattttt agctgtaaat
    16081 aaaaattcaa taactagtaa agtatcatct atttttaatt atataggtgt ttctatccct
    16141 tctttttgga taggtatgat actcatttca attttttcag ttaaacttaa cattttccca
    16201 tcaggaggta tgcatactat tggaaatgat agtattgaag atttagtaaa acatttagtt
    16261 ttgcctgtaa taactttagg tctatataat actgctattt ttacaaatta tgtagaagct
    16321 ggtgtaaatg aacaattaaa aaaacaatat gttgttacag caagagcaaa aggattatca
    16381 gaaaaagtca tactatttaa acatgtcttg aaaaattcac tgacatcatt ggttacaatc
    16441 ttaggcatga gtatacaaaa attagttact ggtgcatttg ttacagaagt agttttctct
    16501 tggccaggta tgggaagatt aatgattgat tcaatttttt caagagatta tactgtaatt
    16561 atggcaataa ctatgttatc ggcactattt ttaattttag gtaatttagt tgctgatata
    16621 ctatacttat taattgaccc taaaataaaa tctagcaaag gaggattcta gttgtgttta
    16681 caagtatctt aaaagaatta aaaaaagata aatcctttgc ttttgcatgt attactctta
    16741 gtataataat catattaatt atggcttctt ttatattaaa tatagactct aatgcaattg
    16801 atactacttc taaacttcaa gaacctagct taaaacatat ctttggtaca gacgaacttg
    16861 gtagagatta cttctctaga gctctttatg gtggaaggat ttcacttagt gttggtattt
    16921 tatcaatgct tatttctgta gtatttggta ctgccgtagg tgttattagt ggatatattg
    16981 gtggtaagct agaccaattc ttgatgagac tagtagatat gcttatgagt ataccaagtt
    17041 tcttagtttt gattgtatta aatacttatc taacacctaa agtttccact attatactaa
    17101 ttattggatt cttaagctgg atggaagttt caagaattgt acgtggcgaa acattaaaaa
    17161 taaaggaaaa tgaatactgt ctagctgcta aagctactgg tatcaaaact agaaatatac
    17221 tattaagaca tatagtttta aacttaaaag agactattgt agttgctggt tcattaaata
    17281 ttgctaatgc tatcttaact gaatctgctc ttagtttttt aggtcttgga atccaattgc
    17341 ctcttgcatc atggggtaat atgcttcaag atgctcaaag acatattttt gacaagttat
    17401 atttagctgt atttccagga ttacttatat ttttaacagt catgagtttg actataataa
    17461 gcaaaaaatt ttatagtaat acttaataaa ttttatatag aaaataaaaa cagtgactat
    17521 ctaaagacat acactgtttt tatttttcgt tttaaaaaaa tacatatttt gtatattata
    17581 actttaaata ctactctacc atgttatgta aaacctacta ttgctctcaa gcacatagat
    17641 gaaaagctaa tagtttatta taataaaaat ggatttattt atttgttact ttaataacat
    17701 ttttaagagt tctttttgct aaagaaatct ataataaatt taaatacatg ttggtctaca
    17761 tggctataac gttctttttt aggtctacat agtataactt tccatgtaat aggatgatta
    17821 aaaggctttt ctataatttt gctattttca aaaaaatcat gaactggtga tggtagaatt
    17881 gttataaaat cagaatttgt ggttgatagc aacaaaaaat cccatgaacc agacattata
    17941 tatcttttta acttaacatt ttcatcagta aatttttgca ttagcttatg atgtatcata
    18001 aaagtaggat taaatattgc aagtaactgc tcattcaaat cgttccaatc tatcttatcc
    18061 ttcttagcaa gtggatgatt tatatttaaa aatgctgtaa gttcatcttc ttgaagcaca
    18121 tgttcagtta ctacactttt atcaatttta tgtggatgta acagtactgc ataatctaat
    18181 tcctgtaaga taagcattct atttaagtca tatgcaccct tctctactat atcaaattct
    18241 atatctggat tatttgcaac taactgtgca actacatctg aaaatgctat tccaagtata
    18301 agtggaggta tccctattct aacttttcct ttaaattgaa ctgaatcttc tcttaaatct
    18361 tccatcatat ttttgtattc atttataata tgttctgcat ttacataaaa tctttctcct
    18421 gctggtgtta acccatttaa cctaccttta tacctttcaa ataaatatac atcttcattt
    18481 tcttcaaaat tttttataat ttgacttagt gctggttgag atatatgaag tgtcttagat
    18541 gctattgata aattaaaacc agatttcact atttcaacaa aatatttcaa ctgttttata
    18601 tccattttaa acccccttcc ccaatagtat aatttagatt atattttttc ttataatgat
    18661 attttatagg aaaatcaaca taatttcatt aatattttaa caaaatattt ttctaaatct
    18721 actctaaatt tttatcctat aaatttttct tatacactat ataaaaaatt agacttagac
    18781 aaaacgcttt tttcgccgta atctataatt agaaacagga aaacctaaag cattaggaat
    18841 aaaaataatt gaaatactta aaaacaagct tgtattaaaa aagtacttta ttatagtgtt
    18901 ccctattttt tttattcaga aaatttttaa ttatcattca agaaaggtaa ctattagata
    18961 tgaaagaggt agtaatcgta tcagcagtca gaacacctat aggaagtttt ggaggtgtat
    19021 ttaaaaatac ttcagctgtt caacttggaa ctatcgctgt taaagaagcg atatcacgtg
    19081 taggacttaa tttatctgaa atagatgaag taataattgg aaatgttctt caaacagggc
    19141 ttggacagaa tgttgctaga caaattgcta taaatgcagg tataccaaat tctgttccaa
    19201 gctacacagt aaataaatta tgtggttcag ggcttaaaag tgttcagcta gctgcccaat
    19261 caatcacatc tggtgaaaat gatgtggtaa ttgcaggtgg tactgaaaat atgagccaag
    19321 ctccttatat cgttccaact gctcgctttg gtagtaaaat gggtgacata accatggtag
    19381 atagtatgct aacagatggc cttattgatg ctttcaatca gtatcatatg ggaataactg
    19441 cagaaaatat tgcaactaaa tttgaattta ctagagaaat gcaagataaa ttggcattag
    19501 aaagtcaaaa taaggctgag aatgctataa aaaacaaccg ctttaaagaa gaaatcgttc
    19561 cagtagatgt tttaatacgt cgaggaaaaa tagaaactat tgataaagat gaatatccta
    19621 aattaggtat gacatttgaa ggtttagcta aacttaaacc tgcattcaaa aaagatggca
    19681 ctgtaacagc tggaaatgcc tcaggtatta atgatggtgc ggctatgtta atactaatga
    19741 gccaacaaaa agcagatgaa ctaggtatta gaccacttgc taaaataaaa tcctatgctt
    19801 cagctggtgt tgaacctgaa gtcatgggta ctggtccagt ccctgcaact agaaaagcac
    19861 tgaaaaaagc tgggcttagc atcaatgata tagacttaat agaagcaaac gaagcatttg
    19921 ccgcccaagc attagctgtt aaaaatgaac ttcaaataga ttcatcaaag ttaaatgtta
    19981 atggtggtgc aatagcatta ggacatccaa taggagcaag tggagctaga atacttgtta
    20041 ccttaattta tgaaatgcaa aaacgtaaag ttgaaactgg acttgcaaca ctttgtattg
    20101 gtggtggtca aggaatatca atggtggttt cacgatagga ggaaatatga ataaaatagt
    20161 tagcattgat gaagctctat ctcatgtaaa agatggagat acaataatgg ttggtggatt
    20221 catggccaat ggttcaccag aaaatttaat tgattattta tgtagtaaaa atataaaaga
    20281 tttgacacta atttgcaatg atactggctt tattgacaaa ggagttggca aaatggttgt
    20341 aaataagcaa tttaaaaaga taattgcttc tcatgtagga ttaaataaag aaactggaag
    20401 acaaatgaac aacaatgaaa cagatgttga gttagttcct cagggaactc ttgctgaaca
    20461 aatacgtgca gctggttatg gtctaggagg tatactcaca ccgactggta ttggtactct
    20521 tattgaggag ggaaagcaaa aaattgttgt cgatagcaaa gaataccttc ttgaaaaacc
    20581 aatttatgca gatgttgctc ttctatttgc atcaaaggtc gataaagcag gtaatctagt
    20641 ttataaaggc tctatgaata attttaataa tttaatggca tctgctgcca aaattacaat
    20701 agttgaagca gatgaaatcg ttgaaattgg cgatattgac ccaaatgaag ttaatacacc
    20761 aggtattttt gtgaattaca tcgttgaagg aggtaattta taatggataa attagaaatg
    20821 caagaatata ttgcaaatag agtatctaaa gaattaaaag acggagccgt tgtaaacctt
    20881 ggaataggtc taccaaccaa agtagcaaat tatatcccgg atgatgtaaa cgttatccta
    20941 caatctgaaa atggcttttt aggtttaggt tcagctgaag atggaaattc aagtgatgaa
    21001 actattgtaa atgcaggtgg tcaaccagtt actatacttc ctggaggatg tttctttgat
    21061 agtgctactt cttttggaat aatcagagga ggtcatgttg atataactgt tcttggcgca
    21121 cttcaggttg acaaatatgg caatatagca aactatatga ttccaggtaa gatggttcca
    21181 ggtatgggtg gagctatgga cctagtaact ggagctaaaa aagttattgt agcaatggaa
    21241 catacttcaa aaggttctgc aaaaatatta aataattgca ctcttccact tactgcaaca
    21301 aatgctgtag atttgatagt tactgaaatg ggagttatgg aagtaacatc agatggaatt
    21361 ttacttaaag aaataaatcc agctttcaca ttagatgatg ttatttctgc tactgaagct
    21421 cctttaatat tgtcagattc attaaacgga aacatatctg ttgtttaaaa taattaaaac
    21481 tagaaaataa aactctctta gacgtattac atataaattt gtattgctta aaatattaat
    21541 ataaaaataa aataaaattg gaggaaatga aaatggttaa agataaagtt gtattcgtaa
    21601 caggagctgc aagtggaata ggtaaacaaa tagctgaatc gttcttaaaa aatggtgcta
    21661 aagtaatgtt ctctgatata aatcaagaag ctttaaataa taccatagca gagttacaaa
    21721 aagaaggtta tgattgcatg agtgtaaagt gtgatgtaac taaggaagaa gaaattaacc
    21781 acgctataga caaaactgtt gaaaagtatg gcagattaga tatattaata aacaatgctg
    21841 gacttcaaca tgtttctatg atagaagatt tcccaactga taaatttgaa tttatgataa
    21901 aaataatgtt aacagcacct tttatagcta ctaaaaaagc atttcctata atgaaaaaac
    21961 aaggctttgg tcgtataata aatatggcct caatcaatgg tgtaatagga tttgctggaa
    22021 aggctgctta taactcagca aaacatggac ttattggact aacaaaagtt gctgctcttg
    22081 aagctgccga ttctggtata acagttaatg ctttatgccc tggatatgta gatactccac
    22141 ttgtcagagg tcagtttaat gacctttcaa aaactcgtaa tataccattg gaaaatgtct
    22201 tagaggaagt tttataccca cttgtaccac aaaaacgcct attagatgtt caagaaattg
    22261 ccgactatgc tatgttccta gcaagtgata atgctaaggg tattacagga caagcttgct
    22321 tactagatgg aggatatact gcacaatagt tattatattt agggcttgaa gaaagtattc
    22381 ttcaagtcct tctactaagg agggatattt atgagtatta tcgcaccaat tggtataatt
    22441 attggtatta tagctattat atatttttca gttaaagaaa ttcatataac tatagctgct
    22501 ccaatagcaa ctttaatagt tgttctctta aataaaatgg atgttgtaac atcaatgcta
    22561 ggtgctgaca aaaacaatta tatgggtgca cttggaaact atataatgag ttattttgca
    22621 atattcttat taggctcaat acttgctaaa tttatggaag aaagtggagc tactgtatca
    22681 atagctgatt ttattttaaa taaatttggc tctaatcatc cttacagagt tttagttgct
    22741 atatttatag taagttttat attaacgtat ggtggtataa gtttatttgt agttatgttt
    22801 gcagtcattc cacttgcaag aactctattt aaaaaacttg atatttcttg gaaccttatt
    22861 cagataccac tttggcttgg tattgcaaca attactatga ctataattcc tggaacacca
    22921 gctattcaaa atgttatacc tattcaatat ctaaatacgt ctcttactgc agctactata
    22981 ccaagtattg taggtagtat aggttgtgct acgtttggac ttgtctacat gaaatatgct
    23041 ctaaacaaaa gtattaaaaa aggtgaaact tatgcaactt acacttcaga attagaagaa
    23101 gaatctttag atagagaatt accaggattt tttgcaagta tttttcctct attagcactc
    23161 gtaattatag ctataactgg aagtactttt ggttcagaat ttatgaaaaa aaatgtaatt
    23221 tacatagctt taatagttgc aattctttta tctgtttttt tattccataa atttctatca
    23281 tctaagatac agacattgag cataggtgct agtggttcta taggtcctat atttgctaca
    23341 tcttctgcag ttgcatttgg agcagtaata atggctgcac ctggttttga tgtatttagc
    23401 aaacttataa tgtcaatacc aggtagtcca cttataagtc ttactgtttt gacttcaaca
    23461 atgtcagcaa taacaggctc ctcttctggt gcattaggaa tagtaatgcc aaactttgca
    23521 gattattatt taagtactgg cctgaatcca gaattaattc accgtgttgc tacaatagcc
    23581 tcaaatatac taactatagt tccacaaagt ggtgtttttc ttacattctt aagcttatct
    23641 aagttaacac ataaaactgg atttaaagaa actttcattg tagttgcagt tggttcacta
    23701 atagcagaaa ttattgtaat tacattagga ttactaatgt attaatataa aacaaattaa
    23761 aattcatgat atgaaaggtt gtgtctcaaa gtgaactttt tacttaatga gacacacttt
    23821 tttgtatgaa ataatatata ttttaatcat ttcaacaata aaaaagaacc tttctctatt
    23881 ttgagacaga ccctttttat tccttaaaga tttaaataat tataatttgt tactaatacg
    23941 aaagcatcta ttgaataaat ttaactaaat aaaaaaaaat ttatcataac tataaattta
    24001 cgttatagaa ttattataaa aatattattt tctctaagta agtaattcca tactatacag
    24061 cttttaacac aaattatcaa acacacttaa atatacctat ttattccata tatatctttt
    24121 ttacatatct ttacagtaaa aaaagtctta atcttatgtt agtatagatt cacgggtacc
    24181 ccctacacta aaggttacaa ttcagtaaca tttttagaat ttttagatta agaggtgttt
    24241 aatgatgaga aaaaatatac caacaaaatt aaaaactata ttagctttag gagtgttaac
    24301 cttagcaaca gcttctagtg ctgttactgc aagtgcttca tcacttgaaa ataatgtaaa
    24361 tataagtaac aaaaatttag tatacagtaa tactaaatac aaattttctg caaaaccaaa
    24421 ttgttctgta aaaccagaac ttaagcctga taaagataac tcttgtaagg ataagaatca
    24481 taatgacaag aattgcaaca acactaataa acctgaggac aacaataatt cagactctac
    24541 taataaacct gataacaata aacctgagtc taataaacct gaggacaaca ataattcaga
    24601 ctctactaat aagcctgata acaataaacc agagtctaat aaacctgaag acaacaataa
    24661 ttcagactct actgataaac ctgataacaa taaaccagag tctaataaac ctgaagacaa
    24721 caataattca gactctacta acaaacctga tgataataac aactcaggct ctacaagtga
    24781 aaatttttca gcttaccaaa aagaagttgt cgacttagta aatgtagaaa gagctaaagc
    24841 tggattaaat cctcttactc ttgattcttc tatatcaaat gttgctacaa aaaaatctca
    24901 agatatgatt gataacaact atttttcaca caattctcca acttatggtt ctccatttga
    24961 catgttgaaa aaatttggaa taagctataa gacagcaggt gaaaacatag ctatgggtca
    25021 aaaaactcct aaagaagttg taaacgcatg gatgaattct gaaggtcaca gaaaaaatat
    25081 aatgaatcca aatttcagta aaataggtgt tggagtagca caaaaaagtg gtggttcaat
    25141 ttattggaca caaatctttg taggataaaa tactcttttt ccatacatat caaaagagta
    25201 tctctaagtg ataaaaaaca caaatgagat actcttttgt atctatttta aaattatcaa
    25261 atccaaatat acatactata aatattattt gttctcttaa ttttagtatg tagaacaata
    25321 tataaaaagt attggcaaga tgaaaatgga aaacttaaaa aagatattta atagataaaa
    25381 acaaaatctt aatatcttca taaaaaaagg actgtctcaa aatagagata aggccttttt
    25441 catttttgaa atgattaaaa tctatttgat ttcatacaaa aagagtgcct catgaactaa
    25501 aaagctcacc ttgagacact ctcttttatc aattctatat aagcattata atattttaat
    25561 attattcagc taattttttg tatccttcat atctttcttt agcatcagct tcagctttag
    25621 caaataatcc atcagcttcc tctgggaatt gtttagctat agcagaatat cttacttgtg
    25681 caagtatgaa atctttgaag cttgcagttg gttcttttga atctaaagtg aatgggtttt
    25741 taccttcagc ttttacttct ggattgaatc tgtataaatg ccagtatcca caagcaacag
    25801 cttgtgcttc gttagctaca gttcttccca taccttcttt taatccatgg tttatacatg
    25861 gagcataagc tattatcaga gatggcccat catgtttttc agcttctaga acggctttga
    25921 taaattgatt tttatcagca cccatagcaa cttgagctac atatacgttt ccatatgtca
    25981 tagccatcat tcctaaatct ttctttctag attttttacc tgaagcagca aacttagcca
    26041 tagcagcagc tggagtagct ttagaagctt gacctcctgt atttgaatat acttctgtat
    26101 caaatacaag tacgtttaca tcttcaccag atgcaagaac atggtctaat ccaccataac
    26161 cgatgtcata tgcccaaccg tctccaccta gtatccattg agatctctta actagataat
    26221 cttttctatc ttttatagct tttactaatt ctttagcttt ttcatctgta caatcacaag
    26281 ctggtttttc taataattca acaaccttag cacttgctac tttagaagct tctccatcat
    26341 ttctagaatc taaccactct gtaaatactg ctttagcatc ttcacagata tccatagaaa
    26401 ctaactctgt catagcatct actattttat ttcttatttg cttaacagct gtgtacatac
    26461 caaatccata ttcagcatta tcctcgaata aagagtttgc ccaagatgga cctttacctt
    26521 catggttaca agtataagga attgatggtg cagaagctcc ccatatagaa gaacatccag
    26581 tagcattagc tatcatcatt ctatctccat ataattgtgt aactacttta atgtatgcag
    26641 tttctccaca tccagcacat gctcctgaaa actccattaa tggttgtcta aattgacttc
    26701 ctttaacagt atttgcaggc ataacatcaa cttttggagc tacttcatca ggatttactg
    26761 caaatgccca attatctaac tctgtatcaa gttgagtatc tattggcttc ataactaaag
    26821 ctttttcttt agctggacat atgtcagcac agtttccaca tcctgtacag tcaagtggac
    26881 taacttgaat tctatatttt aatccttcta atcctttgcc taatgctttt ttagcttcaa
    26941 atccttctgg agcattagct aattcttctt cagtaactaa tactggtctt atacatgcat
    27001 gaggacatat aaaagaacat tggttacatt gaatacagtt atctattatc cattcaggaa
    27061 catcaacagc tataccacgt ttttcataag ctgcagtacc acaagggaat gttccatctt
    27121 ctataccatt aaatgtactt actggtaagt tgtttccttc ttgtgcagtc attggtctta
    27181 gtatattttt tataaattct ggttctttaa cttctccttt atctgcatca acagcatttt
    27241 cccaagaagc tggaacttca actttaacta aagcatttat tcctgcatca acagcggcat
    27301 agttcatatt aactattttt tcaccttttt taccataagc tttaactata gaatctttta
    27361 agtatttaac agcatcttct tctggtatta tttcagctaa tttaaagaat gcagattgca
    27421 ttatcatgtt tattctgttt ccaagaccta tttcttgtcc tagtttaaca gcgttaactg
    27481 tatagaattt tatatcattt ttagctatat attgtttcat atgagctggt aaatgttctt
    27541 caagtcctgc ttggtcccat atagtgttaa gtacaaatgt accacctttt ttaagacctt
    27601 ttaataaatc atattgataa acatatgatt gtttatgaca ggcaatataa tcagcttcat
    27661 ctattaaata agtagctctt attggagtat caccaaatct taagtgagac atagttatac
    27721 caccagattt tttagagtca taatcaaagt aagcttgagc gtatttttca gtatggtcac
    27781 cgattatttt gatagcttgt ttgttagctc caacagtacc gtcagaacct aatccccaga
    27841 acttacatct tatagttcct ttagaagcta tttttattgg ttcagatgga gttaaagaag
    27901 tattagttac atcatcaact atacctactg taaatccatt tttaggttgt tctgaaacta
    27961 agttatcaaa aacagttttt atatctgaag gagtagtatc ttttgaacct aatccgtatc
    28021 ttccacctat tatcatagga gcattttctt ttccatagaa tacatcacgt acatctaagt
    28081 ataatggttc accagttgaa cctggctctt tagttctatc aagtacacat attctttcaa
    28141 cagtagatgg catagcatct aagaaatgtt tcattgagaa tggtctatat aaatgaactt
    28201 taactaaacc atatttttct cccttagcat ttaagtaatc tatagtttct tctattgttt
    28261 ctgttactga acccatagct attaatacgt atttagcatc ttctgctcca tagtaatcaa
    28321 ataaactatg ttttctacct gttaaattac tcattttttc catgtatttt tcaactatac
    28381 ctactatatt ttgatagtat ttattagaag cttctctagt ttggaaatat atatctgggt
    28441 tttgagcagt accacgagca actggatgat ttggagataa agcattatct ctaaatgctt
    28501 gaactgcttc aaagtttaat aaacttgcat aatcttcatt ttctagtaat tcaacttttt
    28561 ggatttcatg tgaagttctg aatccatcaa agaaatgtat aaatggtaat ctaccttcta
    28621 ttgcagctaa atgtgcaaca ggtgctatat cagctacttc ttgaactgaa ccagaagcta
    28681 ataatacaca tccagtttgt ctagcagcca taacgtcttg gtgatcccca aatattgata
    28741 acgcttgaga tgctaaagca cgagcactta catggaatac tcctggtaat aactctcctg
    28801 caactttata catgttaggt atcattaata ataaaccttg agatgctgta aaagtagaag
    28861 ttaaagctcc tccttgtaaa gaaccgtgga atgttcctga tgcacctgct tctgactgca
    28921 tttcaacaac attaactgtt tgtccaaata tattttttct tccttgtgat gcccactcat
    28981 caactacttc agccatagtt gaagatggtg tgattggata tatagctgct acatctgtaa
    29041 acgcataggc aacatgagct gcagctgtat ttccatcaag tgttttcata aacttagcca
    29101 taatatgtat cctccttgat agctacaatt gtacttgttt ataacaagtc tattttttta
    29161 tatttacata taatttacac aacaaaaata taaacacaca tatagtcgcg caaatactct
    29221 atcttctaat ataatactac agattagata aaattccttt gaaaattgta aattaattac
    29281 atatttaagt agattttttt taaaattaaa tatgttaagt ttttttcata attttgtata
//
LOCUS       DAEPXF010000032        27862 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain HB27
            SAMN13376389-rid12479423.denovo.034, whole genome shotgun sequence.
ACCESSION   DAEPXF010000032 DAEPXF010000000
VERSION     DAEPXF010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13376389
            Sequence Read Archive: SRR10518432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 27862)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27862)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001069624.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001069624.1
            Genome Coverage       :: 315x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/15/2021 01:54:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,165
            CDSs (total)                      :: 4,076
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 89
            rRNAs                             :: 10, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 10 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 19 of 69
            Pseudo Genes (incomplete)         :: 44 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 10 of 69
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27862
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13376389-rid12479423.denovo.034"
                     /strain="HB27"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="China: Hebei"
                     /collection_date="2017"
     gene            16..2028
                     /locus_tag="KRL67_001260"
     CDS             16..2028
                     /locus_tag="KRL67_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HBH1522156.1"
                     /translation="MRRNTKLLTTGILSMAIVAPTMAFATESNAMENNADLNINLEKK
                     SIVLGSKSKVSVKFKEKPDADSITLKYKCYDMPLDTTLNYNQSTGAYEGTINYNQDPE
                     YLNVWELQGITINSKNNPKTLNRQDLEKMGLNLKDYNVTQECIIEDITSRKDVNKYLR
                     KTSSPITELTGSDRYETAVKISKEGWKNGSDKVVIINGDVSIDGIISTPLATTYNAPI
                     LLVEKNNVPNSVKSELKRLNPKDIIIIGDENAISKTTANQIKSTVNASQTRLNGSNRY
                     ETSLLIAKEIDKNHDVEKVYITNANGGEVDALTIAAKAGQDKQPIILTDKDSITDNTY
                     KWLKSEDLQNAYFIGGPQMISTNVINKVNGITKDSVTNNRVYGADRHETNANVIKKFY
                     TADELEAVLVAKSDVLVDALAAGPLAANLKSPILITPKTYVSAYHKGNLEAKSANKVY
                     KIGGGLTSKVMSSIASSLSKHNTTPTEPGNSGGKTVMIDPGHGGSAPGNSSGGMIEKD
                     YNLNTSLATTEHLRSKGFNVIMTRDTDKTLSLGNRTALSNSLKPDLFTSIHYNGSTNK
                     QGHGVEVFYKLKDKNGGTTKTVATNILNRILEKFKLTNRGIKTRVLPSDSTKDYLYVL
                     RSNDMPAVLVECAFLDNENDMSLINSSAKVKEMGTQIGKGIEDSLK"
     gene            2270..4183
                     /locus_tag="KRL67_001261"
     CDS             2270..4183
                     /locus_tag="KRL67_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH1522157.1"
                     /translation="MEYLFEKSSLKEYGNEALDKYRELLDKTGNNSRCILLLVPNNNT
                     KIRYERALRLNYSEELKITTYISFVKKELVKYWPLIIEKCDGISTKAVSPTFISNSLS
                     EYLIVQKVKEKRIQEGYFEDITGTNKSIANSIMTNINKSAFNLIDFDFIGEKIYSSKK
                     NKDSIYRFSYTQMDEIISYYINSLLSNSILDNALSIYLYNQYLLNDDFYLKTLFTEVR
                     YIIVDSLESSSNAEVDFITEALDNTLQSYIYFDYSRDYSVFNNVDIDYINEKIISKIK
                     PKEENIKKKEIGIEDLYLLPANIELNQSSQLYNEMLDLISEKVISLIEEDVSPRDIAI
                     ISPINNSILEHQIRDSLIEKNIDVFNTKKDKKAVDYPYGNALVVATCIFYGYLDFIKD
                     DEYLSFLETLLEINRIKAFKIFNETRHLSIDNIDEHSQYRDILQYIEEKKKSDIKIHE
                     FLTQFYIDKMLNLKEGKKNVGLCKKIIVESESFSESLSLLGMKKEKIFIEALKSTIND
                     YYSVVDIEELKSKDKIVITTPYSYISSDIDRSIQIWVDIGSNAWNMKIEKDISNLIVL
                     RKSFEEKKIYTNEMEEYYKKYYLYNTVYNLLLNAKKIYAYKSEYAINGYIQESMLYSI
                     LLKISHKGDKNYD"
     gene            4176..6395
                     /locus_tag="KRL67_001262"
     CDS             4176..6395
                     /locus_tag="KRL67_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HBH1522158.1"
                     /translation="MININYREDQIPIINYDNGTMAVPAVPGAGKTFIITNLVAKLLL
                     EQKHKGGKILVLTYMNSAVNNFKGRIRTILEENKIDDTNSYEVMTIHSLAVKIIKEKP
                     EVVMLSEDFNIADDLQKSIILNECISRFRFEGGERAFRWFLKEQKDERWKEITLEAWE
                     RGFFEFAGNAISELKYKGISPDKLEEILSRGHKGMLKIILPIYKLYDRKLKQNGLLDY
                     DDILILAEKTLSLDEGLRKKFQARYKYIFEDECQDSNEIQGNIIKIISSENKNLVRVG
                     DINQSITGTFSSSDPKFFKEFINSADFCYRMDMSNRSSKDILDLANTLVKYVTKEFRQ
                     KECRHALEDMQIKTVPNGMGYKENPNPEHYNINVKWYESWKIEIEQTARYVKGIKNKY
                     PDKSIGILVPFNEQVTQVAKELRENNLTFEELGPNSLNKRKIINNIAYIIDFMLHCDD
                     VEKLIVVLNKVFIDTDNEEEIKYFLEELKNYSTEEIIYNFEFSKLDNGLIEFNEESDI
                     CLGFKRGIDIIKQILEYPIIRLDLLVLFIGKKLNLEKEDKAVLDYISFYIKYLVSENI
                     NMDLEDVYNLLFDVKNKVFNHIIDVVYEINGYEPEPGSITLCNYHKSKGMEWDCVFLL
                     GLVEYNFPDNINQKFQSDKWYLKEKYKNPMAIIKSEVEAILKGSISTDYTHKTKIDSI
                     NEKIRLLYVGITRAKEMLVLSGSAYRDESDIGNKRKEQKPCIYLNRLNQHIIEKRSN"
     gene            6504..7334
                     /locus_tag="KRL67_001263"
     CDS             6504..7334
                     /locus_tag="KRL67_001263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HBH1522159.1"
                     /translation="MNDKLKYFSYSQNSINTYKSCPLKFKYKYIDRINWKNDDVGSRE
                     YYETLKTGRDFHLICERYFSNIPLGIYFNEDDKNSKKFLKWMENIKKVVPIEKGKTYL
                     PEYEVRMTLNGDIIQAKYDLVVIGNNNIEIWDWKTESKKIDYKHVENRIQTIVYMFLA
                     KEVIPKVLKMDINVKDINMRYYQPEFDDLPITILYDEEKHEANRNKIQNYISMIKNTN
                     YEEHTYEDNLYNDIEKVYNKEERMCYRNKKHCKYCEFNKLCNGKEIDYSILEAEIYGT
                     "
     gene            7324..10791
                     /gene="addB"
                     /locus_tag="KRL67_001264"
     CDS             7324..10791
                     /gene="addB"
                     /locus_tag="KRL67_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddB"
                     /protein_id="HBH1522160.1"
                     /translation="MGLRFVLGRSGSGKSTYILDEIKKEAQKNETTSIILLVPEQYTF
                     EVENRVSKLFLGKEKDKYLRVRVLSFKTLSNIVFSQVGGLTDVNINSSGKAMMVYRAI
                     EDVSEELNVFSKSKSQSGFVSSITDMISEMKQYNISPEMLENISGELDNETLSLKLKD
                     ISKIYNSFEGKLHENYVDAQDMLTSLASKIELSSYLDGACVYIDEFTGFTPNQYNVIK
                     SILNKSKSVNISLTVDDINYIGYSKSDMFSRTKFTYSKLTQLCNEEGIKILPQVNLNT
                     GVIKRFEKVKELQHLERFYNAYPYKIYSNQTENIKIKEFNNLYSEVEEIAREIVHLVR
                     DKNVRYRDITIATRDLSRYDFLVHSIFNEYNIPNFIDKKREAKSNPIVILIISALEMK
                     NRRYGYETMFRYLKSGLIGIDNDDINLLENYVLANGIKGKKWFDEKWDYRITQSLSGK
                     ESEFELELKEKINEIKNKVLEPIVILQEKLRGKNRVKEICRYIYEFLLDINMPETIES
                     LIVNFKDKGELDVANQYSQVWDIVVDILDQMVELMGDEIISLEKFIKLITLGFDEYEL
                     GLVPPSIDQVLVSSVDRMKNPDTKYLYLVGTTDGVFPLITKDSGILSDNDRESLGNKG
                     IEVDIDSKTRSFEEQFLVYKALTSTSKNLTITYPISDHEGKTLRPSIIISRLKKIFPN
                     IENKSYLVEENKSTDEDILKKITVKSPTFNELINVIKNYDSDGYNTEEINSIWLDIYR
                     YYLKDEIYSSITKKVIKGLSYTNQVHKIEEKKIRSLYKSNSLSISRLEKYAECPFAYF
                     IQYGLKARKRKEYSFTPPDLGTFIHNILDRFSKELLQDNLSWRDIDEKYIELKIGIIV
                     DEIILKIPGYILNSSERYKYLAYRLKNMLTTAITIISQQIKQGSFEPIDYEVKFGDNG
                     KYSPIKIVLENGQEVSLIGQIDRVDEFEEGENKYIRIVDYKSGNKSISLTEIYYGLQL
                     QLLVYLDAILESAKDEDININPAAILYCRINNPIAKFNEDKDDEEIQEAILKELRMKG
                     LVVKDSHIVKEMDKSLIDGERKNSLVIPVGLTKDGNVGKSTSAISYEDFKLLRKYVRH
                     AIKDLCEEMLSGEIRIAPYKHKDGTSCDFCDYSAICQFDSTIKDNKYKNLNNKSNEEI
                     IKMMKGDVN"
     gene            10791..14618
                     /gene="addA"
                     /locus_tag="KRL67_001265"
     CDS             10791..14618
                     /gene="addA"
                     /locus_tag="KRL67_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddA"
                     /protein_id="HBH1522161.1"
                     /translation="MSSPKWTKEQLEVIESRECNLLVAAAAGSGKTAVLVERIIQMIT
                     SRENPIDIDKLLVVTFTNAAASEMRERIGDAIGKALDENPENKHLQNQLVLLNKSSIT
                     TIHSFCLDVIKSNFHRVSLDPNFRIGDQTECAILKQEAIEEVFEDLYEERDEGFLNLV
                     ESYAERGGDKEVQDIILGIYSFAMASPEPKKWLIDSAERFNIDENFDFSQSIWARAIL
                     DTVKIEINGLCLNMERALKEVESIEELETFAEKLSVEYKKIADISQACNKSWDEAYKK
                     MASMSFENYVKGVKRISKDAPSYIKESKEKAKTIRDKTKKSLESIVSATFNKDNDSIR
                     EEIKYLYNIVKPISNVVLRFEEEYSNKKREKGIIDFNDIEHFALNILTDVDEKGNIIP
                     SDIAVGYRNKFYEIFIDEYQDSNLVQEVLLKAVANTETPNRFMVGDVKQSIYRFRQAK
                     PELFLQKYNNYNDKKGSSHRKIMLYKNFRSREEVVDAVNYIFENIMNENIGEIEYTEK
                     ERLNLGANFNVDTDEKSIIGGATEIHLIQKDNKLDDDIINDKDDRINNKENEIEEEEK
                     LDNIQLEARMVGNIIKDLMKVNEDGKIQKVYDKGIDGYRPVEFRDIVILLRATSAWAP
                     VFADELMNMDIPTYADVGVGYFDTIEIKTILSLLQIIDNPMQDIPLISVLKSPIFGFT
                     PEDLIDIRVQSKDKIFYEVLKSTAEYDGFTDSQNENESEFIPSEECINKSKDFLIKLK
                     EFKEKSMYMSTDEFIWYLYTRTGYYAYVGALPGGSQRQANLKVLFERAKQFEETSLKG
                     IFNFVNFIEKLKKASSDMGSAKTLGENANVVRIMSIHKSKGLEFPVVICSAMGKNFNT
                     QDFKKSILYHHNLGYGPQFVDYERRISFPSIAKEALKSKINIENLSEEMRVLYVAFTR
                     AKEKLIITGSTRNIQDSIKRWSNGVESLDTISQYEILKGKNFLDWIMPCVLKHRDLSN
                     LLEEVGLDAVFSVEHNSKWYGKLWNKNDILVEKKSDEEKESIEEILEKIDVDSPDSNY
                     YNEIEEKLNYIYPYEFSTRKPATISVTEIKKIQNNYEEELINTIFEQKVILKKPLFIQ
                     NEEEREKISGTERGTIVHLVMEVLDLKNVSSVNDIKSQIRGFVSKGIITEKQASIVNP
                     YKIYKFFASNIGKRMLNAEIINREKSIYAQVNMKDIYIYEKLINNDDKKLYDNESVML
                     RGIVDAYFEEDNQIVLVDYKTDFVNEENINQIIEKYKKQLDLYADIIETLTGKSVKEK
                     CIYLFGVDEAVCY"
     gene            14663..15874
                     /locus_tag="KRL67_001266"
     CDS             14663..15874
                     /locus_tag="KRL67_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HBH1522162.1"
                     /translation="MRFIHTSDWHLGKSLEGHSRIEEQAKFCEEFIKIVESNEIDMVI
                     IAGDVYDTSNPPAQAEKLFYQTVSRLANNGQRCVLIISGNHDNPERLSAITPLAHEQG
                     ILIYGYPLSATIEAKYKGFEITYATQGCTKLNINGENIVVATLPYPSEKRLNEVFSSE
                     DDSEKQKNYSEKVGDIFRSLEENFRSDTINIAVSHIFVIGGESTESERPIQLGGSFLV
                     ERKDLPEKAQYTALGHLHKQQKASERLNAYYSGSPLQYSKDERAYTKGAYIVDIKAGE
                     KPIIEDVYFNNYKPIEVFKCNGIEEALDICEQNQDREIWSYFEINTDEIISQNEIKKM
                     KELLKDIIEIKPIITSCYEQESVDIKEKSMAELFREFYSFSKGVEPKGELMDLFLDII
                     SEEGESADETN"
     gene            15861..19388
                     /locus_tag="KRL67_001267"
     CDS             15861..19388
                     /locus_tag="KRL67_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBH1522163.1"
                     /translation="MRPIRLELTGLNSYIDKQVIDFEKLIERGLFGIFGTTGSGKSTI
                     LDAITIAMYGNISRNTKEYINSVCDKAIISYEFEIGSKNTRRRYIVDRTIARSKTGTK
                     TSNARLIEVLNDNTQNVLADKVVEVNEKVAQVVGLTANDFTRSVVLPQDKFNEFLRLS
                     RADRRDMLERIFNLEKYGRSLGEKVKKRKNLQLQNLKDLKSKLSQYDGITEEVYNNIN
                     QELIELKNLEKDKNNALDLAQKSYEESKTVYEEQLKLEKNELRKNELDLKNNEIKEKI
                     ALVENDSNAKKVDPYISSVQNLEKKIDEDSFAVSNLEKKLVILNQELEVIKNRYEKIN
                     KIKNEEVPKLSEEKIRLQQAIKLEEELVLLDRELKELKESGINLNKTKVELEKVKQVS
                     ESRKDAVTKSIREVEGKIDKVNISAELKQKIFLAYEYEKDYNNVLEEKNQKINKLEEI
                     LKEIENINLKVRYIDKDKNDVNRNLENLSLHLDVLLKKCPGKSADLLLKSEYVTELRN
                     KANNTKENEIKKSSIQDELKIILESKFNTEREVNLLNEKLENNRKNRDDLEKELEELK
                     YLNLASELRRELKENMPCPVCGSKHHENHNITKYDENISFVKEKLEKLEKEKISIRNN
                     IEELNAKVSGYLSIEKMKTKELEDVKGKLGGIPSSQLLKKLDEEQRKLVLLKSNIQEW
                     EKEKESTENKVTLAKEEKNKIEKEELKIRESLNNYKKLTRDLNIEIEGLENKCKKLKQ
                     EYLGLKTITKVSNLLSKVKEIRENEKELELLSTGHSNLLKNRDSLDIKIRENESKLHE
                     IEIELIKARELYVEKKLSRDNKYKEVINITKGDLAKNLLHNVEENICKILEQEESSKK
                     KLEEQRLEYEKNIAEKHNIDGRLKTAKEQYKDQKSILNKLLAENKFESIYAVKRALLD
                     TDAVKKLIEEILEHEEEQKLLSFKIKSSKEKLNGRSIKKEYFEQLKDEIYNLKVEIGN
                     ISKDIGANQNQLITLKDSLDKINDFNKQLKVVEHNVDLLEELDKCVQGNKFVEYVSTN
                     QLKYIALEASKRLDGITKGRYALEIDSTLNFVMRDNFNGGERRSIDTLSGGETFLTSL
                     SLALALSSQIQLKGSAPLEFFFLDEGFGSLDSELLEIVIESLERLHSNNLSVGIISHV
                     EELKNRVPVKLLVSSSEAGIGSKVKIEYS"
     gene            19519..20772
                     /locus_tag="KRL67_001268"
     CDS             19519..20772
                     /locus_tag="KRL67_001268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HBH1522164.1"
                     /translation="MLESPNNLIQIIFLIVLLIGSAFFSASETALMSLSKIRIRYMQD
                     EGVKGAKLVSSLIENPNKLLSSILVGNNVVNIAATSISTSLFIGLMGEKGVALATAVM
                     TVLVLIFGEITPKTIAANNSEKVSLLVSKPIKAIIFILRPIVWIFNIITNIIFKLFGI
                     TNKGAKSFITEEELKTMVNVSHEEGVLEMEEREIINNVFEFGDMQAKNAMVQRIDMVA
                     IDMEDSYDEIIQVFKTEKLSRMPVYEETIDDIVGILNIKDIIFLSDEEIESFDIKNYM
                     REPFFTYEFKKITQLLEEMKLEKSQMAIVVDEYGGTSGLLTIEDLVEVIVGDIEDEYD
                     EEEDEIQVIKEDEYIVDGSTKIGDVNELIGVNLESEEFDSIGGFIIGHLSRLPEENEV
                     IEVDNIRFCIESIEKNRIKKIRIYT"
     gene            20921..21973
                     /gene="ytvI"
                     /locus_tag="KRL67_001269"
     CDS             20921..21973
                     /gene="ytvI"
                     /locus_tag="KRL67_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation integral membrane protein YtvI"
                     /protein_id="HBH1522165.1"
                     /translation="MSILENNFLYKLKNNTIFFCIYTLLFIFIYKAFPYIAPFFLGGI
                     IALMINPISQKLENKFRINKGISTLVLSFLAVAIVSTVTTIIVINSMKELMGFLNNIS
                     ANPEDISNTIMYFLNKINEFMKSFQEIANFDVEQLVNKFSGEVMQMTKNLLTSILGLA
                     TSIPYIIIFIITLFIATYFIAKDLDKIENSFYNMFTVDVRKKVKNVKKEAGLSLVGYI
                     RAYTILMAITFFAIWGSFALFGLKYGLIVGFVGALMDLIPFLGIITIYLPVIVYYFLI
                     KNYFIAISMTVIFFVLSLIREILEPKLVSVNVGLNPLATLAAIFIGIQVKGIIGVIFC
                     LGLVCMHDILKKVDIF"
     gene            22064..23290
                     /gene="pepT"
                     /locus_tag="KRL67_001270"
     CDS             22064..23290
                     /gene="pepT"
                     /locus_tag="KRL67_001270"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HBH1522166.1"
                     /translation="MKQKVVERFLKYVSFDTTSNSQCENCPSSEGQRVLAKYIVEELK
                     TMGVDDVSLDENSYIMATLKGNTDGVDTIGFISHLDTIEDVSGKDIKPRIIENYDGKD
                     IVLNEALNVITYVKDSPELAEFKGDDLIVTDGTTLLGSDDKAGIAEIVTAIEYLINHP
                     EIKHGDIKIGFTPDEEIGRGADLFDVEKFGAKYAYTLDGGIVGELECENFNAANATIT
                     IHGRNVHPGSAKNKMVNAIHIAAEISEMFPADERPETTEGYEGFWHLNSIGGNVENVS
                     MAYIIRDHCREKFENRKSIMIENIEKINKKYDNRVELDLKDSYYNMKEKIEPVMFIVD
                     IAKEAMEELGIKPRLVPVRGGTDGARLSFNGLPCPNIFTGGLNFHGKNECIPVSSMEK
                     ATKLIVRIAEKYAERV"
     gene            23497..24549
                     /locus_tag="KRL67_001271"
     CDS             23497..24549
                     /locus_tag="KRL67_001271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp18"
                     /protein_id="HBH1522167.1"
                     /translation="MKIKSKKIIAFALSLGIMLPSCVYADTISKNQNAQVDMNNYLQG
                     RKSPKEEKLIGEDRFDTAIKISQSGWNNGSERVFLVNSNSLPDALASTPLASKLDAPI
                     LLTNKNSIPYNVTEEIKRLNPAEVILIGSEGAISSSVKESLEDMEVLVSRIGGADREE
                     TSLLLTKQLDDTGDLGVSKVAVVNGYNGLADATSISSPAASDNTAIIYTGKDSIRSEA
                     KNFITQNSTETYIVGGEYSISKKLEGQLVNSERLAGTDRRDTNAKVLEKFYGKSSKVN
                     NMYFAKDGSGREADLVDGLAAGVLASKTKSPVVLASGSLSSAQKSFIKKVKADKFVQV
                     GGGKNSKPYAEALALQ"
     gene            complement(24849..25418)
                     /gene="yfcE"
                     /locus_tag="KRL67_001272"
     CDS             complement(24849..25418)
                     /gene="yfcE"
                     /locus_tag="KRL67_001272"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiesterase"
                     /protein_id="HBH1522168.1"
                     /translation="MKIGIMSDTHGSLLYFEKALNVLSDCDVLLHGGDVLYHGPRNDI
                     PEGYNPKKFIETLNKLENIVIVKGNCDADVDQMVIEHPIQSPYVMSQFGEIRIILNHG
                     YIESEEEIIDKAKKMGGDILVLGHTHVKKLYMDDNLIVINPGSTSIPKDGSHSVAIID
                     IIKTDDEDELELDINLIDINTGNIININD"
     gene            complement(25443..26243)
                     /locus_tag="KRL67_001273"
     CDS             complement(25443..26243)
                     /locus_tag="KRL67_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+)/NADH kinase"
                     /protein_id="HBH1522169.1"
                     /translation="MKRIITINTNQLNKSLETKELLTRKLINAGFEVYSDIYPDTELI
                     ISIGGDGSFLRTVRDFDFPEIPIMGINTGHLGFFPDILPDKIDSFIEAYTKKDYIIQE
                     MSLLNAEVYTTTSGSNMLAVNEVVIRGDKSRTIHLNLSLDNKHIQNFSGDGMIISTST
                     GSTAYNYSAGGSIVDINLELMQITPLHPINTNAYRCFTSSIICSNESVIKIAPEYRFE
                     DSVLIVVDGVEHRFRQIENIKVSISDAKIKLLRMSNYEFWHRVSEKFL"
     gene            26378..27238
                     /locus_tag="KRL67_001274"
     CDS             26378..27238
                     /locus_tag="KRL67_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBH1522170.1"
                     /translation="MFSIRNITKKLGKFKLNNINLELKEGDIVGIIGPNGSGKTTLIK
                     IIMGIIDADEGEIELCNETIENYPISFKNNIGFVYDSLQFYPHLKVKEFRKIVSLFYK
                     NFDRERFDEYLNKFDIEENMHIENLSKGQSEKLMLSSALSHNAKLLILDEPTAGIDPI
                     VRTEIMQYLQDFVKNGSSSVIISTHNTDNLIKIADYLVFINRGNQIFTVKKELIEQEY
                     KIIRANKAELEAIRESIVGVKEYKYYNEALVKVGDSLKVKSLLIEIDKHKVKNPTIEE
                     LMYYYVNEVR"
     gene            27238..>27862
                     /locus_tag="KRL67_001275"
     CDS             27238..>27862
                     /locus_tag="KRL67_001275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 transporter permease"
                     /protein_id="HBH1522171.1"
                     /translation="MFNLIRKDLIIGISSDGIRNLKYILLFFVFYFFLNSISYYTVSI
                     VVSYLIFINTFECDYENDSRIFIRSMPVSIEDIVYSKYLLGVGLIISVTIIVSLLSKL
                     TSLVFFRNMVLNDVFFSVNIFLAILTILLPLIFKFGYGKMKVCGFIVSILIYFVYGSL
                     LKMISMIVYQVKHFNYSKVGGVYLSNYVTDIANAKYINLYSMTFLTII"
BASE COUNT        11677 a         2743 c         4871 g         8571 t
ORIGIN      
        1 agtaaggaga ttaatatgag aagaaataca aaattattaa caacagggat tctttcaatg
       61 gcaattgttg cacctacaat ggcatttgct actgaatcta atgctatgga aaataacgct
      121 gatttaaata taaacttaga gaaaaaaagt atcgttttag gtagcaaatc aaaagttagt
      181 gtcaaattta aagaaaaacc agatgcagat agcattacat taaagtataa atgctatgac
      241 atgccattgg atacaactct aaattacaat caatcaactg gggcatatga aggaactatc
      301 aattataacc aagacccaga atatctaaat gtttgggaac tacaagggat aacaataaac
      361 agcaaaaata atcctaaaac tttaaacaga caagacctag aaaagatggg attaaattta
      421 aaagactata atgtaacaca ggaatgtata attgaagaca taacttctag aaaagatgta
      481 aataaatatt tgagaaaaac ttcttcacct attacagaac ttacaggaag tgatagatac
      541 gaaacagcag ttaaaataag taaagagggc tggaaaaatg gttcagataa agtagttata
      601 ataaatggag atgtaagtat agatggcatt atatcaactc cactggcaac tacatataat
      661 gcgccaatac ttttggttga aaaaaacaat gtgcctaata gtgtaaaatc agaattaaag
      721 cgcctaaacc ctaaagatat aattataatt ggagatgaga atgctatttc taaaactact
      781 gctaatcaaa ttaaatcaac tgtaaatgct agtcaaacac gtttaaatgg ttctaataga
      841 tatgagacat ctttattgat agcaaaggaa atagataaaa atcatgatgt ggaaaaagta
      901 tacataacaa atgctaatgg cggagaagtg gatgcactta ctatagcagc aaaagcaggt
      961 caagacaagc aaccaattat attaactgat aaagatagta ttacagacaa tacatataaa
     1021 tggttaaaga gtgaggattt acaaaatgct tattttatag gtggtcctca aatgatatca
     1081 acaaatgtta taaataaggt aaatggaata actaaggata gtgttactaa taatagagta
     1141 tacggagcag atagacacga aacaaatgca aacgtaataa aaaaattcta tacagctgat
     1201 gagttagagg ctgttttagt agctaaatca gatgtacttg ttgatgcttt agcagcaggt
     1261 ccattggctg cgaacttaaa atctccaata cttataacac caaagacgta tgtatctgca
     1321 taccataaag gtaatttaga agctaaatca gctaataaag tatacaaaat aggaggagga
     1381 ttgacttcta aggtaatgag ctctatagca tcatcattat ctaaacacaa tacgactcca
     1441 acagaaccag gaaatagtgg gggcaagaca gttatgattg acccagggca tggtggttca
     1501 gcacctggaa attcatctgg aggaatgatt gaaaaagatt acaatttaaa tacttcactt
     1561 gcaacaactg aacatttacg ttcaaaggga ttcaatgtaa taatgacaag agacacagat
     1621 aagactttat ctcttggaaa tagaactgct ctatctaatt cattgaaacc agatttattt
     1681 acaagtatac attataatgg ctcaactaat aaacaaggtc atggtgtaga agtattttat
     1741 aagcttaaag ataaaaatgg agggactact aaaactgtag ctaccaatat attaaataga
     1801 attttagaga aatttaaact tacaaataga ggtataaaaa caagagtact tcctagtgat
     1861 tctacaaaag attatttata cgttttaaga agtaatgata tgccagctgt acttgtagaa
     1921 tgtgcatttt tggataatga aaatgatatg agtttaataa actcatctgc aaaagtaaaa
     1981 gaaatgggta cacaaatagg taaaggaata gaagattcat taaaataaat ggaaataaaa
     2041 atatttaaaa acttaatgta aatttatagt tgtaaattta tactaaagtc aaaaaatgaa
     2101 atataagtgt atcaatagta agactgtatg tacatttgca catgtgaatg caagtatata
     2161 tacagtcttt ttttgtagtt aaatgcaata atttgattgt gttttgtaaa atacacaata
     2221 tgatataatt agataatata cacttaattt aattgaggaa ggtgattata tggaatattt
     2281 atttgaaaaa agtagcttaa aagaatatgg aaatgaagct ttagataaat atagagaatt
     2341 attagataaa actggtaata attcaaggtg tattctttta ttagtaccaa acaataacac
     2401 aaagatacga tatgaaaggg ctctaagatt aaattatagt gaagaactga aaataacaac
     2461 atatataagt tttgtgaaga aagaattagt taaatactgg ccactaataa tagaaaaatg
     2521 tgatggaata agtacaaagg ctgtatctcc aacatttata tcaaatagtt taagtgaata
     2581 tttaatagtg caaaaagtta aggaaaaaag gatacaagaa ggatattttg aagatattac
     2641 aggaaccaat aaatccatag caaatagtat aatgactaac ataaataagt ctgcatttaa
     2701 tttaattgat tttgatttta ttggagaaaa aatatattca tcaaagaaaa ataaagatag
     2761 tatatataga ttttcataca ctcaaatgga tgaaattata tcatattata taaattcctt
     2821 attatcaaat tcaattttag ataatgcatt aagcatatat ttatacaatc aatacctact
     2881 taatgatgat ttttatttaa aaaccttatt tacagaagtg agatatataa ttgtggatag
     2941 ccttgaaagc tcaagtaatg cagaagttga ttttataaca gaagcattgg ataatacatt
     3001 acaaagttat atatattttg attatagtag ggattattct gtatttaata atgtggatat
     3061 agactatata aatgagaaaa taatatcaaa aattaagcct aaagaagaaa atattaaaaa
     3121 gaaggagatt ggaatagaag atttatactt attacctgca aatatagaat taaatcaatc
     3181 aagtcaatta tacaatgaaa tgttagattt aataagtgaa aaagttatat ctcttataga
     3241 agaagatgta tctccaagag atatagcaat tatatctcct ataaacaata gtatattgga
     3301 gcatcaaatt agagattctt tgatagaaaa aaatatagat gtttttaata ctaagaagga
     3361 taaaaaagct gtagattatc catatggaaa tgcattggta gtggctacat gtatattcta
     3421 tggatattta gattttataa aagatgatga atacttaagc tttttagaaa ctcttttaga
     3481 aataaacagg ataaaagcat ttaagatttt taatgaaaca agacatttaa gtattgataa
     3541 tatagatgaa catagtcagt atcgtgatat tttacagtat attgaagaaa aaaagaagag
     3601 tgatataaaa atacatgaat ttttaactca attttacata gataaaatgt taaatttaaa
     3661 agaaggtaaa aagaatgttg ggttatgtaa aaagataata gtcgaaagtg agtcttttag
     3721 tgaaagttta agtctacttg ggatgaaaaa agaaaagata tttatagaag ctttaaaaag
     3781 tacaataaac gattattact ctgttgtcga tatagaagag ttgaaatcca aagacaaaat
     3841 agtaataact actccttatt catatatctc atcagatata gatagaagta tacaaatatg
     3901 ggtagatata ggaagtaatg cttggaatat gaagatagaa aaagatataa gtaatttaat
     3961 tgtacttaga aaatcttttg aagaaaaaaa gatttataca aatgaaatgg aagagtatta
     4021 taaaaaatat tatctatata atacagtata taatttgcta ttaaatgcaa agaaaatcta
     4081 tgcatataaa agtgaatatg cgattaatgg ttatatccaa gagagtatgt tatatagtat
     4141 acttttaaaa atctcacata aaggagataa aaattatgat taatataaat tatagagaag
     4201 accagatacc aataataaat tacgataatg gtactatggc agtaccagca gtaccaggtg
     4261 ctggaaaaac atttataata acaaatttag tagctaaact attacttgaa cagaagcata
     4321 agggtggtaa aatattagtt cttacatata tgaatagtgc tgtaaataat tttaaaggta
     4381 gaataagaac tattcttgaa gagaataaaa tagatgatac taattcgtat gaagtaatga
     4441 ctatacacag tttggctgta aaaataataa aggaaaagcc agaagttgta atgttaagtg
     4501 aggattttaa catagctgac gatttgcaaa aaagtattat attaaatgaa tgtataagta
     4561 gatttagatt tgaaggagga gaaagagctt ttagatggtt tttaaaagag caaaaagatg
     4621 aacgctggaa agaaataact cttgaagcat gggaaagagg tttttttgaa tttgcaggaa
     4681 atgcaataag tgaacttaag tataagggaa tttctccaga taaacttgaa gaaatattgt
     4741 caagaggaca caaaggaatg cttaaaatta ttttaccaat atataaattg tatgatagaa
     4801 aattgaagca aaatggattg ctagactatg atgatatatt aattttagca gaaaaaactt
     4861 taagcttaga tgaagggctt agaaagaagt ttcaagctag atacaaatat atatttgaag
     4921 atgagtgtca ggactcaaat gaaattcaag gtaatataat aaaaattata tctagcgaaa
     4981 ataaaaactt ggttagagtt ggagatataa atcagagtat aacaggaaca ttttcatcat
     5041 cagaccctaa attttttaaa gaatttataa atagtgcaga tttctgttat aggatggata
     5101 tgtcaaatcg aagttcaaaa gatatacttg atttagcaaa cactcttgtc aagtatgtta
     5161 caaaggaatt tagacagaaa gaatgtagac atgctcttga agatatgcaa ataaaaactg
     5221 tgccaaatgg aatgggatat aaagaaaatc ctaatccaga acattataat ataaatgtaa
     5281 agtggtatga gagttggaaa attgaaatag agcaaactgc tagatacgtt aagggtataa
     5341 aaaataaata tccagataaa agtataggta tattggttcc atttaatgaa caagtgacac
     5401 aagttgctaa agaacttaga gagaataatc taacctttga agaattagga ccaaattctt
     5461 taaataaaag gaagattata aacaacatag catatattat tgattttatg ttacattgtg
     5521 atgatgtaga aaagttaata gttgtactta ataaagtttt tatagatact gataatgaag
     5581 aagaaataaa gtatttttta gaagaattaa aaaactattc taccgaagaa attatttata
     5641 attttgaatt tagtaagtta gataatggtt taatagaatt taatgaagaa agtgatatat
     5701 gtttaggttt taaaagagga atagatatta taaaacagat attagaatat ccaataataa
     5761 ggttagacct cttagttttg tttattggga aaaaacttaa tctagaaaaa gaagataaag
     5821 ctgtactgga ttatatatct ttttatataa agtatctagt atcagaaaat ataaatatgg
     5881 atttagaaga tgtatataat ttattgtttg atgtaaaaaa taaggtgttt aatcatataa
     5941 tagatgtggt atatgagata aatggatatg aaccagagcc cggtagtata acactatgta
     6001 actatcataa gtcaaagggg atggaatggg attgtgtgtt tttactaggt cttgttgaat
     6061 ataatttccc tgacaatatt aatcaaaagt ttcagagtga taaatggtat ctaaaagaaa
     6121 aatataaaaa ccctatggca attattaaat cagaagttga agctatatta aaaggaagta
     6181 tttcaacaga ttatactcat aaaacgaaga ttgattctat aaatgaaaag attagacttt
     6241 tatatgtagg gattactaga gcaaaggaaa tgttagtact ttcaggaagt gcttatagag
     6301 atgaaagtga tatagggaat aagagaaaag agcaaaaacc atgtatatat ttaaacagac
     6361 ttaatcaaca tattattgaa aaaaggtcaa attaatatat aataagttat taaaagattg
     6421 cttgaaatat atctaaaagg ggataaaaca atatgtacag taagtaacta ctaaaaaata
     6481 aagttaagag agaagtgata tgagtgaacg ataaattaaa atatttttct tatagtcaaa
     6541 attcaattaa cacatataaa tcatgtccat tgaaatttaa atataaatat attgatagaa
     6601 taaattggaa aaatgatgat gttggtagta gagagtatta tgaaacttta aaaacaggaa
     6661 gagattttca tttgatttgt gaaagatatt ttagcaatat acctcttgga atctatttta
     6721 atgaggatga taaaaatagt aaaaagtttt taaagtggat ggaaaatata aaaaaagttg
     6781 ttcctattga aaaaggaaaa acgtatttgc cagaatatga agtaaggatg accttaaatg
     6841 gggatataat acaggcaaag tatgatttag ttgtaattgg aaataacaat atagaaatat
     6901 gggattggaa aaccgagtct aaaaagattg actataaaca cgtagaaaat agaatacaaa
     6961 ctattgttta tatgttttta gcaaaagaag taatacctaa agttttaaaa atggacataa
     7021 atgttaaaga tataaatatg agatattatc aacctgaatt tgatgactta ccaattacca
     7081 ttttatatga tgaagaaaaa catgaagcaa atagaaataa aattcaaaat tatataagta
     7141 tgataaaaaa cactaattat gaagaacata catacgaaga caatctatac aatgatatag
     7201 aaaaggtata taataaagaa gaacgtatgt gctatagaaa taaaaagcat tgcaagtatt
     7261 gtgaatttaa taagttatgt aatggcaaag aaatagatta tagtattttg gaggcggaaa
     7321 tttatgggac ttaggtttgt attaggtaga agtggaagtg gaaaatccac ctatatacta
     7381 gatgagatta aaaaagaagc tcaaaaaaat gaaacgacat caattatact tttagttcca
     7441 gaacaataca catttgaggt tgaaaataga gtaagtaaac tatttttagg aaaagaaaaa
     7501 gataagtatt taagagttag ggtacttagc tttaagacac taagtaatat agttttttct
     7561 caagtaggag gattgacaga tgtaaatata aattctagtg gaaaagcaat gatggtatat
     7621 agagctattg aagatgtaag tgaagaatta aatgtgtttt caaaatcaaa atcgcagtct
     7681 ggttttgtaa gctcaattac agatatgata tcagaaatga aacaatacaa tataagtcct
     7741 gaaatgttag aaaatatatc tggagaattg gataatgaaa ctttaagttt aaaattaaaa
     7801 gatataagta aaatatataa ttcttttgaa gggaaattgc atgaaaacta tgtagatgct
     7861 caagatatgt taacttcatt ggcaagtaaa attgaattaa gcagttattt agatggagct
     7921 tgtgtttata tagatgaatt tacaggtttt acaccaaatc agtacaatgt aataaagagt
     7981 atattaaata aatctaaaag tgttaatatc tctttgactg tagatgatat caactatatt
     8041 gggtacagta aatctgatat gttttccaga actaaattta cttattcaaa attgactcaa
     8101 ttgtgcaatg aagaaggaat aaaaatattg ccacaagtaa atttaaatac aggtgttata
     8161 aaaagatttg aaaaagttaa ggaattacag cacctagaaa gattttataa tgcttatccg
     8221 tataaaatat attcaaatca aactgaaaat ataaagataa aagaatttaa caatttatat
     8281 tcagaggtgg aagagatagc cagagaaatt gtacatttag ttagagataa gaatgtcaga
     8341 tatagagata taaccattgc tacaagagat ttaagtagat atgatttttt agtacactct
     8401 atatttaatg aatataatat accaaacttt atagataaga agagagaggc aaaaagtaat
     8461 ccaatagtta ttttaattat ttcagcttta gaaatgaaga atagacgata tgggtatgaa
     8521 actatgttta ggtatttaaa aagtggactt ataggaattg ataatgatga tataaattta
     8581 ctagaaaatt atgtgttggc taatgggatt aaaggtaaaa aatggtttga tgaaaaatgg
     8641 gattatagaa taacacaaag tctttcaggt aaagaaagtg aatttgaact ggaattaaaa
     8701 gagaaaataa atgaaataaa aaataaagta ttagagccta tagtaatttt acaagaaaaa
     8761 ttaagaggaa aaaatagagt aaaagaaatc tgtagatata tatatgaatt tttattagat
     8821 ataaatatgc cagaaactat agaaagttta atagtaaact ttaaggataa aggggaactt
     8881 gatgttgcaa atcaatactc acaagtttgg gacatagtag tggacatatt agaccaaatg
     8941 gtggaactta tgggtgatga aataatttct ttagaaaaat ttataaaatt aataacctta
     9001 ggttttgatg aatatgaatt aggactggtt cctcctagta tagaccaggt acttgtaagt
     9061 agtgtggata gaatgaaaaa tccagacacg aaatatttat acctagttgg aactactgat
     9121 ggtgtgtttc cactgattac aaaagatagt ggaatattga gtgataatga tagggaaagt
     9181 cttggaaaca aaggaatcga agttgatatt gatagtaaga ctaggagttt tgaagaacag
     9241 tttcttgtat ataaagctct tacttcgaca tctaagaatt taaccataac gtatccaata
     9301 tcagaccatg aaggtaaaac acttagacca tcaataatta tatcaagact taagaagata
     9361 tttccaaata tagaaaataa aagttattta gtggaagaaa ataaaagcac tgatgaagat
     9421 atacttaaaa aaataacggt taaatcccct acatttaatg aacttataaa cgtaataaaa
     9481 aattatgaca gtgatggtta caatacagaa gagattaata gtatttggtt agatatatat
     9541 aggtactatt taaaagatga aatttatagt tctataacaa aaaaagttat aaaaggcctt
     9601 agttatacta atcaagttca taagattgaa gagaaaaaaa taaggtcttt gtataaaagt
     9661 aattctctta gtatatctag gcttgaaaaa tatgctgaat gtccatttgc ttattttata
     9721 caatatggtc taaaagcaag aaagcgtaaa gagtattcat ttacaccacc agatttagga
     9781 acttttattc ataatatttt agatagattt tcaaaagaac ttttacaaga taatttatct
     9841 tggagagata ttgatgaaaa atatatagaa ttaaagatag gtataatagt tgatgaaata
     9901 attttaaaga taccaggata tatattaaat agttctgaga ggtataaata ccttgcatat
     9961 agattaaaga atatgttgac aactgctata actataatta gtcagcaaat aaaacaaggt
    10021 tcatttgaac caatagatta tgaagttaaa tttggcgaca atggaaaata ttcccctata
    10081 aaaatagtgt tagaaaatgg acaagaagta agtttaattg gacaaataga tagagttgat
    10141 gaatttgaag aaggagaaaa taaatatata agaatagtag attataagtc aggcaataag
    10201 tccataagtc taacagaaat atattatggt ctacagttac aattacttgt gtatttagat
    10261 gcaatccttg aaagtgcaaa ggatgaagat ataaatataa atccagctgc aatactatat
    10321 tgtagaataa ataatccaat agcaaaattt aatgaagata aagatgatga agaaatacaa
    10381 gaagcaatat taaaagaact tagaatgaag ggacttgtag taaaagactc tcacatagtt
    10441 aaagagatgg ataaatcatt aatagatgga gaaaggaaaa actctttagt aataccagta
    10501 ggtcttacta aggatggaaa tgtaggtaaa agtacttcag caataagcta tgaggatttc
    10561 aaactgctta gaaaatatgt aaggcatgct ataaaggatt tatgtgaaga aatgttaagt
    10621 ggagaaatac gtatagctcc atataaacat aaagatggaa catcatgtga cttttgtgat
    10681 tactctgcta tatgtcagtt tgattcaact ataaaagata acaagtataa aaatttaaat
    10741 aataagagca atgaagaaat aataaaaatg atgaaaggag atgttaatta gtgagttctc
    10801 ctaaatggac aaaagagcaa ttagaagtta tagaaagtag agaatgcaat ttattggttg
    10861 ctgctgctgc tggttcagga aaaacagccg tgctagttga gcgtataatt caaatgataa
    10921 caagtagaga aaatccaatt gatatagata agttattagt tgttacattt actaatgctg
    10981 ctgcgtcaga aatgagagag aggatagggg atgctatagg aaaagcctta gatgaaaacc
    11041 cagaaaataa acatttacaa aaccaacttg ttttattaaa taaatctagt attacaacaa
    11101 tacactcatt ctgtttggat gttataaaat caaactttca tagagtaagc ttagacccta
    11161 acttcagaat aggagaccaa actgaatgtg ctattttgaa acaagaagca atagaggaag
    11221 tatttgaaga tttatacgaa gaaagagatg agggtttttt aaatttggtt gaaagttatg
    11281 cagaaagagg aggagataaa gaagtacaag acattatctt gggaatatat tcatttgcaa
    11341 tggcttctcc tgaacctaaa aaatggttga ttgactcagc agaaagattt aatattgatg
    11401 aaaattttga tttttcacag tctatttggg ctagagctat attagacact gtaaaaatag
    11461 agattaatgg tctttgtcta aatatggaaa gggcattaaa agaagtggaa tcaatagaag
    11521 aattagagac ttttgcagaa aagttatctg ttgaatataa aaaaattgct gacatttcac
    11581 aggcatgtaa taaatcttgg gatgaggcat ataaaaagat ggcaagtatg tcttttgaaa
    11641 attatgtaaa aggagtaaaa agaatttcaa aagatgctcc ttcgtatata aaagaatcaa
    11701 aagaaaaggc aaaaactata agagataaga ctaagaagtc tttagaaagt atagtaagtg
    11761 ctacattcaa taaagataat gattctatta gagaagaaat aaaatatctt tataacatag
    11821 ttaaaccaat atctaatgtt gtactgagat ttgaagagga atatagcaat aagaaaagag
    11881 aaaaaggaat aatagatttt aacgatatag agcattttgc attaaatata cttacagatg
    11941 tagatgaaaa aggtaatatt ataccttctg atatagcagt tggatataga aacaagtttt
    12001 atgaaatatt tatagatgaa tatcaggata gtaatctagt gcaggaagta ctgcttaaag
    12061 ctgttgcaaa tacagaaact ccaaatagat ttatggttgg tgatgtaaaa cagagtatat
    12121 atagatttag acaagcaaag ccagaattat ttttgcaaaa atataataac tataatgata
    12181 aaaagggaag ttcccataga aaaataatgc tttacaaaaa ctttagaagt agagaagaag
    12241 ttgtagatgc tgtaaattat atatttgaaa atataatgaa tgagaatata ggagaaattg
    12301 aatatacaga aaaagaaaga ctaaatttag gtgcaaattt taatgtggat acagacgaga
    12361 aatctattat tggtggagca acagaaatac acctaataca aaaagataat aaacttgatg
    12421 atgatattat aaatgataaa gacgatagaa ttaataacaa agaaaatgaa atagaggaag
    12481 aagaaaaatt agataatata caacttgagg ctagaatggt tggaaacatt atcaaagatt
    12541 taatgaaagt caatgaagat ggaaaaattc aaaaggtcta tgataaggga atagatggat
    12601 atagacctgt ggaatttaga gatatagtta ttcttttaag agctacatct gcatgggctc
    12661 cagtgtttgc agatgaattg atgaatatgg atataccaac atatgctgat gttggagtgg
    12721 ggtattttga tacgattgaa ataaaaacaa tattgtcatt acttcagata atagataatc
    12781 caatgcaaga tatacctctt atatctgtac taaaatcacc tatatttgga tttacaccag
    12841 aggatttgat tgatattaga gttcagagta aagataaaat tttttatgag gttttaaaaa
    12901 gtacagcaga gtatgatgga tttacagatt ctcaaaatga aaatgagtct gagtttatac
    12961 caagtgaaga gtgcataaac aaaagtaaag attttttaat taagctaaag gaatttaaag
    13021 aaaagtcaat gtacatgagt acagatgaat ttatatggta tttgtacaca aggactggat
    13081 actatgctta tgttggagca ttgccaggtg gaagtcaaag acaagctaat ttaaaagtat
    13141 tgtttgagag agcaaaacaa tttgaagaaa ctagtcttaa aggaatattc aactttgtaa
    13201 attttataga gaaattaaaa aaagctagtt ctgatatggg aagtgcaaag acacttggtg
    13261 agaatgcaaa tgttgttaga ataatgagta ttcataaaag taaagggcta gaatttccag
    13321 tagtcatatg ctctgcaatg ggtaaaaatt tcaatactca agattttaaa aagagtattt
    13381 tatatcatca taatttaggg tatggacctc aatttgtaga ctatgagaga aggatttctt
    13441 ttccaagtat agccaaagaa gcattaaaaa gcaaaataaa tatagaaaat ttatcagaag
    13501 agatgagagt tttatatgtt gcatttacaa gagctaaaga aaaattgatt ataacaggtt
    13561 ctactagaaa tatacaagat agtataaaga gatggtcaaa tggagttgaa agtttagata
    13621 caatatcaca atacgagata ttaaaaggta aaaatttttt agattggata atgccttgtg
    13681 tattaaaaca tagggattta tctaatctac tagaggaagt aggattagac gcagttttta
    13741 gtgttgaaca taattcaaaa tggtatggga agttgtggaa taagaatgat attttagtgg
    13801 aaaagaagag tgatgaagaa aaagaaagta ttgaagaaat tcttgaaaaa atagatgtag
    13861 atagtcctga cagtaattat tacaatgaaa tagaagaaaa attaaactat atatatccat
    13921 atgaatttag tacaagaaaa cctgctacta tatctgttac agaaataaaa aagatacaaa
    13981 ataattatga agaagagtta ataaatacta tatttgaaca aaaagtaata ttaaaaaagc
    14041 ctctatttat tcaaaatgaa gaagaaagag aaaaaataag tggaacagaa agaggcacaa
    14101 tagtccattt agttatggag gttttagact taaaaaatgt aagtagtgta aatgatataa
    14161 aatcacaaat aagaggtttt gtttcaaaag gcataataac agaaaaacaa gctagtatag
    14221 taaatccata taaaatatat aaattttttg catctaacat aggaaaaaga atgttgaatg
    14281 ctgaaattat aaatagagaa aaatcaattt atgcacaagt aaatatgaaa gacatatata
    14341 tttatgaaaa gcttataaac aatgatgata aaaaacttta tgataatgaa agtgtcatgt
    14401 taagaggtat agttgatgct tattttgaag aagataatca aatagttttg gtggattata
    14461 aaacagattt tgtaaatgag gaaaatataa atcaaataat agagaaatat aaaaaacagt
    14521 tggatttata tgcagatatt attgaaactt taactggtaa gtcagtaaaa gagaaatgta
    14581 tatatttatt tggagttgat gaagctgttt gttattaatt ttttatataa attaaaccta
    14641 aaagatagag gaaggggaaa aaatgagatt tattcataca tcagattggc atctagggaa
    14701 atccctagaa ggccattcta gaattgagga acaagcaaag ttttgtgagg aatttataaa
    14761 aatagtagaa tcaaatgaaa tagatatggt tataattgct ggagatgtat atgatacttc
    14821 taatccccca gcacaagcag aaaaactatt ttatcagact gtatcaagac ttgctaataa
    14881 tggtcagagg tgtgtattaa taatttcagg aaatcatgat aatccagaga gactttctgc
    14941 aataacgcct ctagcacatg aacaagggat acttatatat ggttatccat taagtgctac
    15001 gatagaagct aagtataaag ggtttgaaat aacatatgca actcagggat gtacaaaact
    15061 gaatataaat ggagaaaata ttgtggttgc tacattacct tatccaagtg aaaaaagatt
    15121 aaatgaagtt ttttcaagtg aagatgattc tgagaaacaa aaaaattatt ctgaaaaggt
    15181 aggagatata tttagaagtt tagaagaaaa ttttagaagt gatactataa atatagccgt
    15241 atcacatata tttgtaatag gtggagagag tactgaatca gaaagaccta tacaattagg
    15301 tggaagtttt ttagttgaaa gaaaagattt gccagaaaag gctcaatata ctgcactagg
    15361 tcatttacat aaacaacaaa aagcttccga acgtctaaat gcatactatt ctggttctcc
    15421 acttcaatat agcaaggatg aaagagcgta taccaaaggg gcatacatag tggatataaa
    15481 agctggtgaa aaaccaataa ttgaagatgt atattttaat aattacaaac ctatagaagt
    15541 atttaaatgt aatggaatag aagaagcact agatatatgt gagcaaaatc aagatagaga
    15601 gatttggtca tattttgaaa taaatactga tgagataata tcacagaatg aaattaaaaa
    15661 aatgaaggaa ctattaaaag acataataga aataaaacca ataataacat cttgttatga
    15721 acaagaaagt gtagatataa aagaaaaatc tatggcagag ttgtttagag agttctattc
    15781 ttttagtaaa ggtgtagagc caaaaggaga acttatggat ttatttttag atataataag
    15841 tgaagaaggt gaatcagcag atgagaccaa ttagattaga acttacaggg cttaatagct
    15901 atattgataa acaagttata gactttgaaa aattaattga aagaggtttg tttgggatat
    15961 ttggaactac tggaagtgga aaatctacaa tcttagatgc aataactatt gctatgtatg
    16021 gaaatatatc cagaaataca aaagaatata taaacagtgt gtgcgataag gcaattatat
    16081 catacgagtt tgagatagga agcaaaaaca ctagaagaag atacattgta gatagaacca
    16141 ttgctagaag taaaacagga acaaagacct ctaatgctag attaatagaa gtattgaatg
    16201 acaataccca aaatgtttta gctgataaag ttgtggaagt aaatgaaaaa gtagcgcagg
    16261 ttgtaggatt aacggcaaat gattttacta ggtcagtagt tttaccacaa gacaaattta
    16321 atgagtttct aagattatca cgtgcagata gaagagacat gttagagaga atttttaatc
    16381 ttgagaaata tggaagaagt cttggagaaa aagtgaaaaa gagaaaaaat ctacagttac
    16441 aaaatttaaa agatttgaaa tcaaaattaa gtcaatatga tggaattaca gaggaagtat
    16501 acaacaatat aaaccaagaa ttaatagaat taaaaaattt ggaaaaagat aagaataatg
    16561 ctttagacct agctcaaaaa tcttatgaag aaagtaaaac agtatatgaa gaacaattaa
    16621 agttagaaaa aaatgaactt agaaaaaatg agcttgattt aaaaaataat gagataaaag
    16681 aaaagattgc tcttgtagaa aatgatagta atgctaaaaa ggtagaccca tacataagtt
    16741 cagttcaaaa tctagaaaaa aagatagatg aagatagctt tgcagtaagt aatttagaaa
    16801 agaaattagt aatattaaat caggaacttg aagttataaa aaatagatat gaaaaaataa
    16861 acaaaattaa gaatgaagaa gtccctaaat tgagtgaaga aaaaataaga ttgcaacaag
    16921 caataaaatt agaagaagag ttagttttat tagatagaga gttaaaagag ctaaaagaaa
    16981 gtggaattaa tttaaataag actaaagttg aattagaaaa agtaaagcag gtgtcagagt
    17041 caagaaaaga tgcagttact aaatctataa gagaagttga aggaaagatt gataaagtaa
    17101 atataagtgc agaattaaaa caaaaaatat ttctagcata tgaatacgag aaagattaca
    17161 acaacgtatt ggaagaaaaa aatcaaaaga taaataaact agaagaaata ctcaaagaga
    17221 tagaaaatat aaatttaaaa gttagatata ttgataaaga taagaatgat gtaaatcgta
    17281 atttagaaaa tttatcactt cacttggatg ttcttcttaa aaaatgtcct ggtaagagtg
    17341 cagatttact tttaaaatct gaatatgtta ctgaattaag aaataaagca aataatacta
    17401 aagaaaatga aattaagaaa agtagtatac aagatgaatt aaagataata cttgaaagta
    17461 agtttaatac tgaaagggaa gtaaatttac taaatgaaaa actggaaaat aatagaaaga
    17521 acagagatga cctagaaaaa gaattagaag aactaaagta tttaaattta gcctctgaac
    17581 ttagacgtga attaaaagaa aatatgcctt gtccagtttg tgggtcaaaa catcatgaaa
    17641 atcataatat aactaagtat gatgaaaata tatcttttgt caaagaaaaa ctggagaaat
    17701 tagaaaaaga aaaaataagc ataagaaaca atatagagga attaaatgct aaagtaagtg
    17761 ggtatttatc tatagaaaaa atgaagacta aagaacttga agatgtaaaa ggaaaactag
    17821 gggggattcc atctagtcaa ttattaaaaa aacttgatga agaacaaaga aaacttgtat
    17881 tgttaaaatc taatattcag gagtgggaga aagaaaaaga gagtactgaa aataaagtaa
    17941 cattagctaa agaagaaaaa aataaaattg aaaaagaaga attaaaaata agagagagtc
    18001 tgaataatta taagaaatta acaagagatt taaacataga aatagaaggt ttagaaaata
    18061 aatgtaaaaa acttaaacag gagtatctag gattaaagac gattactaaa gtctctaatc
    18121 tattgtcaaa agtaaaagaa ataagagaga atgaaaaaga attagaatta ctaagtactg
    18181 ggcattcaaa tttattaaaa aatagagact cactagatat taaaataaga gaaaatgaga
    18241 gcaaactaca tgaaatagag atagaattaa taaaggctag agaattatat gtagaaaaga
    18301 aattgagtag agataataag tataaagaag ttattaacat aacgaaaggt gatttagcta
    18361 aaaatctttt acataatgtt gaagaaaata tatgtaagat attagaacaa gaagaatcaa
    18421 gtaagaaaaa attggaagag caaagattag aatatgaaaa aaacattgct gaaaagcaca
    18481 atatagatgg tagacttaaa actgctaagg aacaatataa agaccaaaaa tcaatactaa
    18541 ataaactatt ggcagaaaat aaatttgaaa gtatatatgc agtaaaaaga gctttattag
    18601 atactgatgc agtaaaaaaa ttgattgaag aaatactaga gcatgaagaa gaacaaaagt
    18661 tattatcatt taaaataaaa tcatcaaaag aaaaattaaa tggaagaagc ataaagaagg
    18721 agtactttga acagttaaaa gatgaaattt ataatttgaa agtagaaata ggaaatatat
    18781 ccaaagatat tggagctaat caaaatcagt taataacatt aaaagactct ttagataaga
    18841 taaatgattt taataaacaa cttaaggtag ttgaacacaa tgtggactta ttggaagaat
    18901 tagataaatg tgtacaagga aataaatttg tagaatatgt ttctacaaac caattaaaat
    18961 atatagcgtt ggaagcatca aaacgattgg atggaataac aaaaggaaga tatgcacttg
    19021 aaattgactc aacactaaac tttgtaatga gagataactt taatggagga gaaagaagaa
    19081 gcattgacac tctatctggt ggagagacat tcttgacatc tctatcatta gcacttgctc
    19141 tttcatctca aatacaatta aagggaagtg caccattaga atttttcttt ttagatgaag
    19201 gttttggttc attagatagt gaacttttag aaattgttat agaatccttg gaaagattgc
    19261 atagtaataa tctaagtgtg ggtataataa gtcatgtcga ggaacttaaa aatagagttc
    19321 ctgttaagct attagtttca tctagtgagg ctggaatagg ttcaaaagta aaaattgaat
    19381 atagttagaa aatatatgtt aattgaatat attatctaat atttccaaat aatatgtaat
    19441 tgtgatataa taaaaactaa tattaaagtt atttataatt aatattaata taaaaacatg
    19501 taagtaggag tgtggtaagt gttggaatcg cccaataatt tgattcagat tattttttta
    19561 atagtattac ttatagggtc tgcatttttc tcagcatctg aaacggcttt aatgtcgttg
    19621 agtaaaatca gaatcagata tatgcaagat gaaggagtta aaggagctaa gttagtaagt
    19681 tcattaatag aaaatccaaa taagctttta agttctatat tggttggaaa taacgtcgta
    19741 aatatagcag caacatccat atcaacatct ttgtttatag gattaatggg agaaaaagga
    19801 gtggcacttg ctacagctgt tatgacagta ttagtattaa tatttggtga gattactcca
    19861 aaaacaatag cagcaaataa ttcagaaaag gtttctctat tagtatctaa acctataaaa
    19921 gcaattatat ttattttaag accaatagta tggatattta atataattac aaatattata
    19981 tttaaattat ttggtataac aaataaagga gctaaatcat tcataactga agaagagcta
    20041 aaaactatgg taaatgtaag tcatgaagaa ggcgtccttg aaatggaaga gagagaaatc
    20101 ataaataatg tttttgaatt tggagatatg caagctaaaa atgcaatggt acaaagaata
    20161 gatatggtag ctatagatat ggaagatagt tatgatgaga taatacaggt atttaaaact
    20221 gaaaaattaa gtagaatgcc tgtgtatgaa gaaactatag atgatattgt aggaatactt
    20281 aatataaaag atattatatt tttatctgat gaagaaatag agtcatttga tattaaaaat
    20341 tatatgagag agcctttttt cacgtatgag ttcaaaaaaa taacacaact tcttgaagag
    20401 atgaaacttg aaaaaagtca gatggctata gttgtagatg agtatggtgg aacatctgga
    20461 cttttaacta tagaagattt agtagaagtc attgttggag atatagagga tgaatatgat
    20521 gaagaagagg atgaaataca agttataaaa gaagatgagt atattgtaga tggaagtact
    20581 aaaattggtg atgttaatga acttattggt gtaaacttgg aatctgagga gtttgattcc
    20641 ataggaggtt ttataattgg acacctaagc agattgccag aagaaaatga agtaattgaa
    20701 gttgataata taagattttg tatagaaagt atagaaaaaa atagaattaa aaaaataaga
    20761 atatatacat aatgtaataa tgttcttaaa tttattagaa gtgtataaat ataaaagata
    20821 acctttatat ttctgcactt ctttttattt tatattcaaa taaaactagt atgaaatgga
    20881 caaaataaaa atataaatta gaagaaagga gatttttaag atgagcatac tagaaaataa
    20941 ttttttatac aaattaaaaa acaacactat ctttttttgt atatatacat tgttatttat
    21001 ttttatttac aaagcttttc catacatagc tccatttttt ctaggtggta taatagcact
    21061 tatgattaat cctatatcgc aaaagttaga aaataagttt cgtataaata aaggtatctc
    21121 tacgcttgtt ctaagttttc ttgcagtggc tatagtcagt acagttacta caataatagt
    21181 aataaattca atgaaagaat taatgggatt tttaaataat atatctgcta atcctgagga
    21241 tataagtaat actattatgt attttttaaa taaaataaat gagtttatga aatcatttca
    21301 agaaatagcc aactttgatg tagaacaatt agtaaacaaa ttcagtggtg aagtaatgca
    21361 aatgacaaaa aatcttctta ctagtatact tggattggct acttctattc catatattat
    21421 aatatttata attaccttgt ttattgctac atactttata gcaaaagacc ttgataagat
    21481 tgaaaatagt ttttataata tgtttactgt tgatgttaga aagaaggtaa aaaacgtaaa
    21541 gaaagaggca ggtctttcac ttgttggata tataagggcc tatacaatac ttatggctat
    21601 aacatttttt gcaatctggg ggagttttgc tttatttgga ttgaaatacg gtctaatagt
    21661 agggtttgta ggagctttaa tggatttaat tccatttcta ggtataataa caatatatct
    21721 tccagtaatt gtttattatt ttcttataaa aaattatttt atagctataa gtatgactgt
    21781 gatatttttt gttttgtctt tgattagaga aatacttgaa cctaaactag tatcggtaaa
    21841 tgttggacta aatccattag ctaccctagc agcgattttt attggaatcc aagtaaaagg
    21901 aataattgga gtgatttttt gtttgggttt ggtatgtatg catgatatac ttaagaaggt
    21961 ggatattttt taattataat aattgatagt ttttttaaaa ataaaacttt attcactggt
    22021 aaataaaaaa attattaatc ttattaagaa agagggtata tacgtgaaac aaaaagtagt
    22081 ggaaagattt ttaaaatatg tatcatttga tacaacatct aattctcagt gtgagaattg
    22141 tccatcttca gaaggtcaaa gagttttagc taagtatata gttgaagagc taaaaacaat
    22201 gggtgttgat gatgtgagtt tagatgaaaa tagttatata atggctactt taaaaggaaa
    22261 tactgatggt gtggatacta taggattcat atcacattta gatacaattg aagatgttag
    22321 tggaaaagat attaaaccaa gaataataga aaattatgat ggaaaagaca tagttttaaa
    22381 tgaagcgtta aatgttatta cgtatgtaaa ggattctcca gagttggcag aatttaaagg
    22441 tgacgattta atagtcactg atggaacaac tctacttggg tctgatgata aagctggtat
    22501 agctgagata gtgactgcaa tagagtatct aataaatcat ccagaaatta agcatggaga
    22561 tataaaaata ggatttacac cagatgaaga aattggtaga ggtgctgatt tatttgatgt
    22621 tgaaaaattt ggagcaaaat atgcatatac tttagatgga ggaatagttg gagaacttga
    22681 atgtgaaaat tttaatgcgg caaatgcaac tattactata catggtagaa atgttcatcc
    22741 aggttcagct aaaaataaaa tggtaaatgc tatacatata gctgctgaaa tatcagaaat
    22801 gttcccagca gatgaaagac cagaaacaac agaaggatat gaaggatttt ggcatttaaa
    22861 ttctataggt ggaaatgtcg agaatgtaag tatggcttat attattagag accattgtag
    22921 agaaaaattt gaaaatagaa aatctattat gatagagaat atagaaaaaa taaataagaa
    22981 atacgataat agagtggaat tagatttaaa agactcttat tacaatatga aagaaaagat
    23041 tgagccagtt atgtttatag ttgatatagc taaagaagct atggaggaat taggtataaa
    23101 accacgttta gttcctgtaa gaggtggtac tgatggagca agattatctt ttaatggatt
    23161 accttgtcca aatatattta ctggtggatt aaacttccac gggaaaaatg agtgtatacc
    23221 agttagttct atggagaaag ctactaaact aattgtaaga atagctgaaa aatatgctga
    23281 aagagtttag ttaaaaagta tttaaaaatt aagtatttaa agttataata gctatatcat
    23341 tctttataag tcaagctgat tataagcttg tattgtattt aaaatggtat agctattttt
    23401 atgattattt ttataaaaga taattatttt tagtttaggg tatcaacaaa aagtatgaat
    23461 atcaataggt attaacattt aaaaggggga attgtattga aaattaaatc taaaaaaata
    23521 atagcatttg cactttcact tgggattatg ttgccaagtt gtgtatatgc agacactatt
    23581 agtaaaaatc aaaatgcaca ggtagatatg aataattatc ttcaaggtag aaaatctcca
    23641 aaggaggaaa aacttattgg tgaagataga tttgatacag cgataaaaat atctcaaagt
    23701 ggttggaata atggttcaga aagggtgttt ctagttaact caaattcatt gccagatgca
    23761 ttggcttcaa ctcctttggc aagtaaattg gatgcaccaa tactgcttac aaataaaaat
    23821 agtataccat ataatgtaac agaggaaata aaaaggctta atccagcaga agttatttta
    23881 attggaagtg aaggagctat ttcatcaagt gtaaaagaat ctcttgaaga tatggaagtc
    23941 ttagtcagta gaataggtgg agcagataga gaggaaacat ctttacttct tactaagcag
    24001 ttagatgaca ctggtgactt aggtgtctca aaagtagctg tggttaatgg atataatgga
    24061 ctggcagatg caacttcaat atcaagccca gcagcaagtg ataatacagc tataatatat
    24121 acaggtaaag atagcataag aagtgaggca aagaatttta ttacacaaaa ctcaactgag
    24181 acatacatag taggtgggga gtacagtata agtaaaaaac ttgaaggaca gttagtaaac
    24241 tcagaaagac tagcaggaac agatagaaga gatactaatg caaaagtttt agaaaagttt
    24301 tatggaaaat caagtaaggt taataatatg tattttgcaa aagatggaag tggcagagaa
    24361 gctgatttag ttgatgggct tgctgctgga gtattagcat caaaaacaaa gtcacctgta
    24421 gtacttgcaa gtggaagttt aagtagtgca cagaaaagtt ttataaaaaa agtaaaggct
    24481 gataaatttg tacaagttgg tggaggcaaa aatagtaaac catatgcaga agcattagcc
    24541 ttacagtaat attgtgtaaa tcaaatttaa tatataataa tagcaagtaa accaagttgc
    24601 acttcaatat aatatataat aaaaacaagt aaactaagct atgtttcaat ataatatata
    24661 atagaaacaa gtaaactaag ttatatttca atatagcata taataaaaca agtaaaccag
    24721 agtctattta attagaagtt taaggtgttt tacttaaact tctaattgag atagaaataa
    24781 aatggtttac ttgtttttta ttatgagttt aataaattaa agcttatttt atttttatta
    24841 aaaatttact aatcattaat atttattata tttccagtat tgatgtcaat taagtttata
    24901 tcaagttcaa gctcatcctc atcatcagtt tttatgatat ctataattgc gacagaatgg
    24961 cttccatctt ttgggataga agtacttcct ggatttatta ctattaaatt atcatccatg
    25021 tataactttt ttacatgagt atgtcctaaa actaatatgt caccacccat tttctttgct
    25081 ttgtcaataa tttcttcttc tgattctata taaccatgat tcaatataat tctaatttca
    25141 ccaaattggc tcatgacata aggactctga ataggatgtt ctataaccat ttggtcaaca
    25201 tctgcatcac agttcccttt tacaatcaca atgttttcta atttatttaa tgtctcaata
    25261 aattttttag gattatatcc ttctggaata tcatttctag gtccatgata taaaacatct
    25321 ccaccatgta ataaaacatc acaatcagat aatacattta atgctttttc aaagtaaagt
    25381 aagctaccat gagtatcact cattattcct attttcatat taaaaatcct cctaagtaca
    25441 gtctataaaa atttttcaga tactctatgc caaaactcat agtttgacat tctaagtaat
    25501 tttatttttg catcagatat actaactttt atattttcga tttgtctaaa tctgtgctca
    25561 actccatcaa cgacaatcaa tacagaatct tcaaatctat attctggtgc aatctttata
    25621 acagattcat ttgaacatat tatacttgat gtaaagcacc tatatgcatt ggtatttatc
    25681 ggatgtaaag gtgtaatctg cataagttca agatttatat caacaatact acctccagca
    25741 gaatagttat aggctgttga acctgtggaa gtagaaataa tcataccatc tccactaaaa
    25801 ttttgtatat gtttattatc aaggcttaaa tttaggtgta tagtcctaga cttgtctcct
    25861 cttataacaa cctcatttac agcaagcata ttagaaccac tagtagttgt atatacttcc
    25921 gcattaagaa gtgacatttc ttgaataata taatcttttt ttgtataagc ttcaataaaa
    25981 ctatcaatct tatctggtaa aatatcagga aaaaacccaa gatgccctgt atttattccc
    26041 attatgggaa tctcaggaaa atcgaaatct ctaactgttc ttaaaaaaga gccatcacca
    26101 ccaattgata taataagctc tgtatctgga taaatatctg aatacacttc aaagccagca
    26161 ttgataagtt ttctagttaa gagttcttta gtctcaaggg acttatttaa ttggtttgta
    26221 tttatagtta taattctttt catatattca cctcttcata ctttgtatta taacatagtt
    26281 ttataattct taatttataa atttataaat gacctttgta gtgaatgtgt taaaatatgt
    26341 ataggtgggt ataaaaaaat gcataagagg tgaagatgtg tttagcataa ggaatatcac
    26401 aaaaaaatta ggaaaattca agttaaataa tataaactta gagttaaagg aaggggatat
    26461 tgttgggatt ataggaccaa atggttcggg aaaaacgact ttaataaaaa ttataatggg
    26521 gattattgat gcagatgaag gtgaaattga actttgtaat gaaactattg aaaattatcc
    26581 aataagcttt aaaaacaata ttggttttgt atatgattct ttacaatttt atccgcattt
    26641 aaaggttaag gaatttagaa aaatagtttc tttattttat aagaattttg atagagaaag
    26701 gtttgatgaa taccttaata agtttgatat agaagaaaat atgcatattg aaaatttatc
    26761 taaaggtcaa tctgaaaagc ttatgttatc aagcgcactt agtcacaatg ctaagttatt
    26821 gattttggat gaaccaactg ctgggataga cccaattgta agaactgaaa taatgcagta
    26881 tttacaagat tttgtgaaaa atggaagtag tagtgtaata atttctactc ataatactga
    26941 taatttaatt aaaattgctg attatcttgt ttttataaat agaggaaatc agatttttac
    27001 ggttaagaaa gaattaatag aacaagaata taagataatt agggcaaata aagcagagtt
    27061 agaagcaatt agagaaagca tagttggtgt gaaagagtat aaatattata atgaggcact
    27121 tgttaaagtt ggagatagct tgaaagtaaa aagcttattg atagaaattg ataagcataa
    27181 ggttaaaaat cctactatag aagaactaat gtattattat gtaaatgagg tgagatgatg
    27241 ttcaatttaa taagaaaaga tttaataata gggattagtt cagatggtat tcgtaattta
    27301 aagtacatat tgctattttt tgtgttttat tttttcctta attcaatttc atattataca
    27361 gtaagtattg ttgttagtta tttgatattt ataaatacgt ttgagtgtga ttatgaaaat
    27421 gattcaagga tttttataag gagtatgcca gttagtatag aagatatagt ttactcaaaa
    27481 tatttattag gtgtaggact aataatttct gtaacaataa ttgtgtcctt actaagtaaa
    27541 ttgacatcac tagtattctt tagaaatatg gttttaaatg atgtattttt ttctgtaaat
    27601 atatttttag caattttgac gattttactt cctttaattt ttaagtttgg atatggaaaa
    27661 atgaaagttt gtggttttat agtaagtatt ttaatttact ttgtatatgg aagtcttttg
    27721 aagatgatta gtatgattgt atatcaagta aagcatttta attatagtaa agttggagga
    27781 gtatatcttt caaattatgt aactgatata gctaatgcta agtatataaa tttatatagt
    27841 atgacatttt taacaataat aa
//
LOCUS       DAEPXF010000033        27826 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain HB27
            SAMN13376389-rid12479423.denovo.035, whole genome shotgun sequence.
ACCESSION   DAEPXF010000033 DAEPXF010000000
VERSION     DAEPXF010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13376389
            Sequence Read Archive: SRR10518432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 27826)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27826)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001069624.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001069624.1
            Genome Coverage       :: 315x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/15/2021 01:54:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,165
            CDSs (total)                      :: 4,076
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 89
            rRNAs                             :: 10, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 10 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 19 of 69
            Pseudo Genes (incomplete)         :: 44 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 10 of 69
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27826
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13376389-rid12479423.denovo.035"
                     /strain="HB27"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="China: Hebei"
                     /collection_date="2017"
     gene            <1..172
                     /locus_tag="KRL67_001276"
     CDS             <1..172
                     /locus_tag="KRL67_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HBH1522172.1"
                     /translation="KENVKLINNKNILLIDDIFTTGLTTNEISKLLKLSGANKVFVLT
                     LLTKSNDNYIME"
     gene            504..2417
                     /locus_tag="KRL67_001277"
     CDS             504..2417
                     /locus_tag="KRL67_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HBH1522173.1"
                     /translation="MICLNKRQENLLTTLLLKEEFINVNKIANDFECSERTIRNDCKY
                     IDEWLSTFCNAYIKRKPNIGIKFSGDGTDKILVNQKLRGEVKRVNSELHKQVEILNLL
                     LCNNKKVTLNDLSNKLYINKNIVREEISKLSVALNNYNLNVSTKKGSGIYIEGDEKDI
                     RIMLVSFLFKYIDKYRLNIDEIEYFHIVDIIIVKNIMFTIEEDMDIRLTDISFKQLML
                     FLLINIIRIRLGNSLKVSNTKKELKLKMLILDIEDELKSNLSIVLNYEEKLFIESLIL
                     GMNKQINSSNIKKSLYLNEELASYTKKIINLVSEESGINFNNDILLYEQLICHLNVTM
                     HQMKSNVYLENPLLDNIKTKFCFLFTVISSSIEIELKGKQITEDEIGYLTLHFQTSLE
                     RKYSKRESNKKASIVCPFSFGVSMLLKVKIEKRFDNIKIIETLREEDLKRDNFNKTID
                     FIIAFQEYENIQKPIFITTPLFTDEDENKLKEFTNKIKEKDTSYKLMNRLMMSDYLIR
                     ELEHDDIYEAITYLTNLLIEKHYAEKEYLQSIITREKSYPTNVGKGILFPHGDMKYIK
                     QSVLCFARLKTPIRIRNGKDIKIILLLAYKKDDDRKVFRELFKEISNLTEDENMLDIL
                     SKCDIEKVKDILI"
     gene            2558..2866
                     /locus_tag="KRL67_001278"
     CDS             2558..2866
                     /locus_tag="KRL67_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HBH1522174.1"
                     /translation="MEEIVLKIIIHAGNAKSMLYEALDYAKENDFKKADELIENANEE
                     ILKAHKVQTELIQKEAGGDKSDISILLIHSQDHLMTCMSERNLIKEMIMLRKEIQKLK
                     "
     gene            2890..3216
                     /locus_tag="KRL67_001279"
     CDS             2890..3216
                     /locus_tag="KRL67_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBH1522175.1"
                     /translation="MKRKVYLFCSFGMSTSLLADKMQKVADEHNLPIEVEAFPIAEID
                     KIVEEKNPDCILLGPQVKYMLKELKPKFEAQGKLIDIINEVDYGTMNGEKVLKLAIKL
                     IKSKQA"
     gene            3255..4589
                     /gene="celB"
                     /locus_tag="KRL67_001280"
     CDS             3255..4589
                     /gene="celB"
                     /locus_tag="KRL67_001280"
                     /EC_number="2.7.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HBH1522176.1"
                     /translation="MNRLERILMPLAEKIGKNKLLIAIRDGFLISSPLLIIGSLFLLV
                     ANFPIKGWNEFVGQIFGSDWAVKLQQPTVATFEVMTLLAVLGIGYSYAKQIDVDPIAS
                     AAVAIVAFFIITPFVIPYTPEGTESVYMVTGIPLGWMGSKGMFVGMITAITSVKLFAA
                     VVKKGWTLKMPDGVPPTVSKSFAALIPSAIVMVVFFLVKIAFEATPYGNVHEFIFNFL
                     QIPLLKLGNTLSATAIAYVFLHLFWFFGINGSSVVGAVYNPILKILSAENLAAFQAGT
                     KLPNIITAQFQDMFATFGGAGSTLSLIIAMLLICKSKRIKSIGKLSILPGIFGINEPL
                     IFGLPIMLNPLLLIPFALVPTVNIIIAYFCMSTGLVPLTNGVQLPWTTPIIFSGFLTT
                     GWQGAVLQLVLLILGIFMYIPFIKILDKQYLKDEKSNVEEEDDDISFDDLVL"
     gene            4600..5118
                     /locus_tag="KRL67_001281"
     CDS             4600..5118
                     /locus_tag="KRL67_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine/betaine/sarcosine/D-proline family
                     reductase selenoprotein B"
                     /protein_id="HBH1522177.1"
                     /translation="MKKIVMILDQIQAGAGGKEKSNIPPAGKSSPLGPGVMMEQFLNE
                     SKVIATLFCGDEFFVNNQEEVTSKMIAMVKKLNPDVVICGPSFNYENFSKMSAILSKN
                     INDKTDIPAFAAMSEENIDVINEYKNDICIVKTPKKGGIGLNDSLNNICKLAKAMANK
                     EDITLMKEEFCY"
     gene            5214..6173
                     /locus_tag="KRL67_001282"
     CDS             5214..6173
                     /locus_tag="KRL67_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N(4)-(beta-N-acetylglucosaminyl)-L-asparaginase"
                     /protein_id="HBH1522178.1"
                     /translation="MWGMIATWRMALEGITEASEHLEKGMEAGDAVELAVRRVEDYPF
                     YKSVGYGGLPNENCEVELDAAFMDGNTLDIGAVAGIRDYSNPVSIARKLSYEKVNNFL
                     VGIGAEEYAHKNGFERKNMLTDRAKLHYKKRKKETLDKGLSPYAGHDTVGMISLDKTG
                     KMCAATSTSGLFMKKRGRIGDSALSGSGFYVDSSIGGATATGLGEDLMKGCISYEIVR
                     LMKEGKHPQEACDIAVNELDKQLIKRRGKAGDLSLVAMNNKGEWGVATNIADFTFSVV
                     TKEESPTVYISNRVDGKTIHKVISNEWMEAYEARIKAPIEVSE"
     gene            6237..7289
                     /locus_tag="KRL67_001283"
     CDS             6237..7289
                     /locus_tag="KRL67_001283"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sapep family Mn(2+)-dependent dipeptidase"
                     /protein_id="HBH1522179.1"
                     /translation="MNIISNKIDELKEDLLSDIIDIVKIPSVRGESENGFPFGEKVGD
                     ALNKALEISEKLGFKVTNLDNYIGYAEYGDSEDYVCVIGHVDVVHEGEGWKYHPYKGE
                     ETNGRIYGRGVLDNKGPIMSALYGLYAIKELNLKLDKRVRIIFGTNEESGFEDIPYYL
                     EKEKAPIMGFTPDCKYPVVYGEKGMAKIRIKSKSNYEEETFLGFIESMSENVLVTYKE
                     LNIENSGIILDIKVKYDFSYKLKDVLDEIKSSFPNSIEIEVISNFNPVYFDKESNLVK
                     KLQLAYERVTSLDGTPVTTNGGTYAKVMPNIVPFGPSFPGQKGIAHNPDEYMDIEDII
                     LNAKIFANAIYELAKE"
     gene            7292..7966
                     /locus_tag="KRL67_001284"
     CDS             7292..7966
                     /locus_tag="KRL67_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HBH1522180.1"
                     /translation="MLEIIGMSAEDAKIIEDCGADRIELVSALTEGGLTPSFGLIEKV
                     VNSVKIPVNVMIRHHAKSFVYSKEDISIMQKDISVVKEIGANGVVFGVLDKNNNIDEK
                     NLNVLLKCCDNLDVTFHRAIDESNTIDSVKILKDYDKITNILTSGGKGSIVDNIQMIK
                     NMMLNSNHIKILLGGGLNFNNIEKIKELTKASNFHFGTAIRINNSPFEEIDRQKLKQL
                     VNIISR"
     gene            8520..9071
                     /gene="raiA"
                     /locus_tag="KRL67_001285"
     CDS             8520..9071
                     /gene="raiA"
                     /locus_tag="KRL67_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HBH1522181.1"
                     /translation="MNIIISGKQIELTDAIKNKIETKLSKLDNYIHPDTDVKVTVSAR
                     KARQKIEVTIATVNGPIIRAEDMQENLYTAIDVVYDKLSKQLKKYKKRLQDKHKDNKS
                     IRFQDAEINLADEQEDYGDENQELVIQRRKKFSVKPMSEEEAVLQMELIGHDFYMFKN
                     IDSDEIAVVYKRNNGGYGIIEHE"
     gene            9203..11878
                     /gene="secA"
                     /locus_tag="KRL67_001286"
     CDS             9203..11878
                     /gene="secA"
                     /locus_tag="KRL67_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HBH1522182.1"
                     /translation="MSFMDNLFNMADKKELKKFNKTVDIIDSLEPKFESMADSELKNM
                     TNIFKERLANGESIDDILPEAFAVVREVSKRVLGLRHYRVQMIGGIVLHQGRIAEMKT
                     GEGKTLVATAPVYLNALTGKGVHVVTVNDYLAKRDRDQMAKIYEFLGMSVGVIIHGQN
                     PKVRKEQYDCDITYGTNNEYGFDYLKDNMVIHKEQRVQRGLNYAIVDEVDSILIDEAR
                     TPLIISGPGDKSTHLYSDANTFVLTLKPDDYELEEKDKAVSLTASGIQKAEVYFNVDN
                     ITDISHTELYHHINQALRAHVIMKKDVDYVAKDGEIVIVDEFTGRLMFGRRYSEGLHQ
                     AIEAKEGLKIQRESKTLATVTFQNYFRMYKKLSGMTGTAKTEEEEFKAIYKMDVFQVP
                     TNKLMIREDLPDCVYKSEIGKFNAVAQEIIERHKVNQPILVGTVSIEKSELLSQILKK
                     KGIKHEVLNAKHHDKEAEIIAQAGRLGAVTIATNMAGRGTDIVLGGNPDFLTKREMRR
                     NGFKEEIVNRVDTPIEGIPVKGNEILFEAREEYEKLFEKFKQQTQEEQKQVVEAGGLA
                     IIGTERHESRRIDNQLRGRAGRQGDPGSSRFYIGLDDDLMRLFGSDRISGIVDKIGLE
                     EDMPIEHRILSKSIEGAQKKVEGKNFGIRKHVLQYDDVMNKQREIIYAERKRVLEGED
                     LQEQIQSMTHSIIEEAVTLYTQDKGFDEEGFKEHMYNLFLPKGSIEIPEIEKLNPVEI
                     TEKVYEIAMKIYTSKEEQVGYERMREVERVILLQAVDNHWIDHIDAMDQLRQGIGLRA
                     VGQQDPVIAYKMEGFDMFDEMNKHIKEDTVRYLFNITIETPVERKAVVDVENLSSPSD
                     GTLPTSKTVKKDEKVGRNDLCPCGSGKKYKNCCGR"
     gene            11893..13000
                     /gene="prfB"
                     /locus_tag="KRL67_001287"
     CDS             join(11893..11967,11969..13000)
                     /gene="prfB"
                     /locus_tag="KRL67_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_153309863.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HBH1522183.1"
                     /translation="MLKLQEYRHSMEQMASNLEELRVSLDIASLMKNIQINEEKMNKQ
                     DFWNDNEVAQRVLQENKSLKETLEEYESLKSLLEDIEVLIEIGLEEDDDSVERDIEKS
                     IESMEEKLSEMKIKTLLNGEYDKNNAILSINAGTGGLDAQDWAQMLLRMYIRWSESKG
                     YKVKLLDIISDPEAGIKTATILVEGTNAYGYLKSEKGVHRLVRISPFDPSGKRHTSFA
                     SIDVTPELDENIEVEINPSDLKIDTYRASGAGGQHVNTTDSAVRITHIPTGVVVQCQN
                     ERSQHLNKDRAMRLLMAKLIELKELEQKEKIEDIQGKYSQITWGSQIRSYVFQPYKLV
                     KDHRTNAEFGNVDSVMNGNIDLFINEYLKMNKIV"
     gene            13099..15240
                     /locus_tag="KRL67_001288"
     CDS             13099..15240
                     /locus_tag="KRL67_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011010799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding transcriptional accessory protein"
                     /protein_id="HBH1522184.1"
                     /translation="MDINQILKKEFNLRDEQINNTLKLIDEGNTIPFIARYRKEMTGE
                     MSDVTLREFYEKLMYLRNLQSRKDDVVRLIDEQGKLTDEITQNIEKAKTLQEVEDIYA
                     PYKQKKRTRATIAKEKGLENLALSILENNLDNIEIEAKNYLDEEKEVLSIEDALKGAR
                     DIIAELVSDDAKIRKYIRELALREGMIVSKSATDEKSVYDMYYDYSEAVKSMAPHRVL
                     AINRGEKENFLKVKLEINNDKVLNYIINEYVNDKNFKNKEEIVSSIEDSYKRLIFPSI
                     EREIRNHLTEIAQERAISVFGKNVKSLLLQPPVKDKVVMGFDPAFRTGCKIAVVDKNG
                     KLLDYTTVYPTDPQNDVEGAKKVLKGLIEKYDIDIISIGNGTASRESETFVSEMIKEI
                     DSEVQYVIVSEAGASVYSASELANEEHPDINVSIRGAISIARRLQDPLAELVKIDPKS
                     IGVGQYQHDLNKKRLEEVLDGVVEDSVNSVGVDLNTASYSLLEHVAGISKAIAKNIIA
                     YREENGDFTSRAQLKKVKRLGPQAFTQCAGFMRILEGKNPLDNTGVHPESYDICKKMI
                     EIIGYSLDDVKNKNIGEIDEKIKEIGLRELSEKLEVGQVTLKDIITEIKKPGRDPREE
                     GIKPILRTDVLKIEDIQEGMTLKGTIRNVVDFGAFVDIGIKNDGLVHKSEMSNSFVKD
                     PMSIVTVGDIVDVKVIGIDLNKKRVALSMKK"
     gene            15388..16554
                     /locus_tag="KRL67_001289"
     CDS             15388..16554
                     /locus_tag="KRL67_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HBH1522185.1"
                     /translation="MIFIKNGKINTITNGIIHGDILIDEGKIIEIGEDLIAPLDVEVI
                     DASNKLVFPGFIDAHTHLGLWEDGIGFEGADGNEETDPITPQLNPIDGINPMDRTFKE
                     AFEGGITSVCTTPGSANVMGGQCIAIKTCGKRIDKMVIKNPVASKIAFGENPKSCYGQ
                     DDKSPQTRMAIAALLRENLKKAEEYLEDIDMYESHDDEDCEKPEYDIKMESLIPVLRR
                     EIPFKAHAHRADDMFTAIRIAKEFNLKLTLDHCTEGHLIVDELVEEGFPVIVGPSLSE
                     RSKFELRNLTFNTAGILSNAGLDVCIMTDHPVIPVQYLPICAGIAVKHGMKEEKAIES
                     ITINPAKTLGIEDRVGSIEVGKDADLVIWDNSPLEIQSNVLYTIINGKVVYEKK"
     regulatory      16614..16731
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            16856..17461
                     /locus_tag="KRL67_001290"
     CDS             16856..17461
                     /locus_tag="KRL67_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HBH1522186.1"
                     /translation="MQNTAKRGNMISTRTLVVMSILSAISYLLMFIQAPIPGIFPDFL
                     KIDLSDIPAIFGGMSLGPLVGFGIVVVKNVLQAITATTTGGIGEFANILIGGSYVMIL
                     CYSYKRSGDLKGVLIGFVLGTISMTIIGSLVNYFVMLPLYGQLMGLDAIIGLGSAINP
                     QVHDLFTFVIWMIAPFNVLKAVVISLVTLPLYKKMGNIIKK"
     gene            17790..18830
                     /locus_tag="KRL67_001291"
     CDS             17790..18830
                     /locus_tag="KRL67_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4116 domain-containing protein"
                     /protein_id="HBH1522187.1"
                     /translation="MYLSKKIKKLFLDDKGYYFNSNWKDIHIGIYDTKLGDSLKYITN
                     EYIENMERVKYDYRELKNIENQTDGMCILAIRQNYKALEYVKNQTEELCLEAIKYNYK
                     ALEYVKEQTEYLCLEAIKKDCNALKYVRNKTEGLIIKAISHSSNIDVVSILKALETQT
                     RRICLEAIKKDGRCLAYVREQSEELCIEAIKQNYKALKYVKNQTEKMCIESVRQNGMA
                     LQYVNKQTDKICIEAVKQDGRSLQFVNNKTEEICINAIRYLNKKYNIKDVLSYIDKYT
                     EDICIEIVRQNGKMLMYIKNQTEKMCIEAVKENYKSLKYVKEQSERICKEALKQNHKA
                     KEYVKIAIDDCI"
     gene            18969..19421
                     /gene="tsaE"
                     /locus_tag="KRL67_001292"
     CDS             18969..19421
                     /gene="tsaE"
                     /locus_tag="KRL67_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HBH1522188.1"
                     /translation="MAKIYLENENKTREIGYKLGKLLKEGSVICLVGDLGAGKTTMTQ
                     SLADSLGIEDYITSPTFTIINEYEGKIPLYHFDVYRIGSSDEMYDIGYDEYVNSNGIC
                     IIEWANLIEDILPKEYLNIELKYKDEGREMILTPKGEFYKEIVEELIK"
     gene            19421..20137
                     /gene="tsaB"
                     /locus_tag="KRL67_001293"
     CDS             19421..20137
                     /gene="tsaB"
                     /locus_tag="KRL67_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HBH1522189.1"
                     /translation="MKILGMDTSSMAASVAVVEDNNLICEFTVNNKKTHSQKLMPMIE
                     NMLSMSDLSIKDMDLLAVCIGPGSFTGLRIGMATVKAMAHVNNIPIIAVNSLESLANN
                     INFCDRKICCILDAQKNQVYSAKYKFENGEMIELDGVDVVEFDALVEEIVSTNEEFII
                     VGEAVYKYKDKLENIENIKIPSPANNVSNAGSLCSLALNKYSKNIDVHTCYTINPMYI
                     RKSQAEVQYDEKMKRLNNGR"
     gene            20127..20603
                     /gene="rimI"
                     /locus_tag="KRL67_001294"
     CDS             20127..20603
                     /gene="rimI"
                     /locus_tag="KRL67_001294"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HBH1522190.1"
                     /translation="MEDNIKLIVDDIKIEEMTTEDIDEVFEVEKNCFEDYWSKESFRK
                     ELSNEVARYIVAKLNGKIVGYVGIWLILDEGHINNVAVHSDYRGKKIGDKLIKGIVDL
                     CKDNNIASMTLEVRASNKIAQNLYRKYGFKMGGIRKEYYSDNKEDAIIMWNQLKEV"
     gene            20609..21625
                     /gene="tsaD"
                     /locus_tag="KRL67_001295"
     CDS             20609..21625
                     /gene="tsaD"
                     /locus_tag="KRL67_001295"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HBH1522191.1"
                     /translation="MSDIITLAIESSCDETAASVLKNGREVLSNIISTQIETHKKFGG
                     VVPEVASRKHVENIDIVVQEALDKANIGFNDIDHIAVTYGPGLVGALLVGLSYAKALA
                     YTLNIPLVGVNHIEGHLSANYIEHKDLKPPFITLIVSGGHTHLVEVKDYGKYEILGKT
                     RDDASGEAFDKISRAMNLGYPGGPIIDNLAKKGNKHAIEFPRAYLEEDSYDFSFSGLK
                     SSVLNYLNGKRMKNEEIVVEDVAASFQEAVVEVLSTKALKAVKEKGYNIITLSGGVAS
                     NSGLRAKITELAKDSGITVKYPPLILCTDNAAMIGCAGYYNFINGKTHDMSLNAVPNL
                     KINQ"
     gene            21922..24630
                     /gene="hpdB"
                     /locus_tag="KRL67_001296"
     CDS             21922..24630
                     /gene="hpdB"
                     /locus_tag="KRL67_001296"
                     /EC_number="4.1.1.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate decarboxylase large
                     subunit"
                     /protein_id="HBH1522192.1"
                     /translation="MSQSKEDKIRSILEAKNIKSNFQNKENLSEFNEKKASKRAEDLL
                     DVYYNTLSTADMEFPYWYNREYRKSDGDIPVVRRAKALKAAFSHMTPNIIPGEKIVMQ
                     KTRHYRGSFPMPWVSESFFVAQGEQMREEAKKLASNTADELTKFGSGGGNVTESFGNV
                     VSIAGKFGMRKEEVPVLVKMAKEWVGKSVEDLGFHYEKMMPDYDLKENLMSTLICMFD
                     SGYTLPQGREVINYFYPLNYGLDGIIEMAKECKKAVAGNASGDGLIGMDRLYFYEAVI
                     QVIEGLQTWILNYAKHAKYLESIETDLEAKKEYSDLVEILEHIVHKQPRTFREALQLT
                     YTIHIASVNEDAISGMSIGRFGQILYPWYEQDIEKGLITKEEVIELLELYRIKITCID
                     CFASAGVNGGVLSGNTFNTLSIGGLKEDGSTGANELEELLLEASMRCRTPQPSLTMLY
                     DEKIPEDFLMKAAECTKLGSGYPAWVNNSNGTTFMMKQFADEGMTVEEARAFALGGCL
                     ETSPGCWKQLTLNGKTYSIAGGAGQSAGSGVHFIANPKILELVLMNGKDHRMNIQVFE
                     PHNKPLDTYEEVIEVFKDYYKQAINVLERANNIELDIWRKFDTSVINSLLKPDCLDKG
                     QHIGNMGYRYNATLNVETCGTVTMVNSFAALKKLVYDDKAFTIEEMKDAILNNFGFKD
                     ALEVGNYSMADQVKVDKTGKYDAIYKACLDAPKYGNNDLYADNILKNYEVWLSKVCEE
                     AQSLYAKKMYPCQISVSTHGPQGAATLATPDGRLSGTTYSDGSVSAYAGTDKNGVYAL
                     FESATIWDQAVVQNSQMNLKLHPTTIKGQQGTKKLLDLTRSYLRKGGFHIQYNVVDSE
                     TLKDAQKNPDNYRQLMVRVAGFTQYWCELGKPIQDEVIARTEYEGV"
     gene            24634..24891
                     /gene="hpdC"
                     /locus_tag="KRL67_001297"
     CDS             24634..24891
                     /gene="hpdC"
                     /locus_tag="KRL67_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate decarboxylase small
                     subunit"
                     /protein_id="HBH1522193.1"
                     /translation="MRKHSDCMNFCAVDATKGICRLSKQMINLDDAACPEIKVMPKCK
                     NCKNFVEANDEGIGKCVGLEKEDWVYSTLNAITCEGHVFNE"
     gene            24884..25834
                     /gene="hpdA"
                     /locus_tag="KRL67_001298"
     CDS             24884..25834
                     /gene="hpdA"
                     /locus_tag="KRL67_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate decarboxylase activase"
                     /protein_id="HBH1522194.1"
                     /translation="MSSQKQLEGMIFDVQSFSVHDGPGCRTTVFLNGCPLSCKWCANP
                     ESWTVRPHMMFSELSCQYENGCTVCHGKCKNGALSFNLDNKPVIDWNICKDCESFECV
                     NSCYYNAFKLCAKPYTVDELVQVIKRDSNNWRSNGGVTFSGGEPLLQHEFLHEVLLKC
                     HEVNIHTAIETSACVSNEVFNKIFKDIDFAFIDIKHMDREKHKEQTGVYNDLILENIS
                     NLANSDWNGRLVLRVPVISGFNDSAENISDIISFMHKNNLVEINLLPFHRLGESKWIQ
                     LGKEYEYSDKGDVDEGHLEELQDMFLDNGIACYVGHETAF"
     gene            26142..27080
                     /locus_tag="KRL67_001299"
     CDS             26142..27080
                     /locus_tag="KRL67_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HBH1522195.1"
                     /translation="MDKSRSNLIIKNKDKKVRSGWIIIAVMATFYILQYYSSTLLMEI
                     LRKILIARGDINLKTNYFSEYANWLNDFGLPIVFQILTDALMITIPIIVWRFIMKNPI
                     SEMGLHSYKIKKKEGYVGMLLGIFNCTVIFIITITIGGGHVISWVPKISPLTVSWILV
                     FILIAFAEEILNRGFFMAVLRRCKNIYFIMIVPSVIFGLIHIWNPDVTFLSVINIIIV
                     GILFSYMFIKSSNIWMCIGYHFAWNVFQGIIYGMPVSGLQVPGIITTQITHGNLLNGG
                     MFGIEGGILTTFVNLLSFIFVWYYYRDSKYDFVSNK"
     gene            complement(27202..>27826)
                     /locus_tag="KRL67_001300"
     CDS             complement(27202..>27826)
                     /locus_tag="KRL67_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HBH1522196.1"
                     /translation="LTFISSLSIIIYSSNKKNLYPVKNIKLGSLLIIYFMISVIVPTS
                     FILILQGAQPNYSGIIKFIFTPITSLTTIYIFFSEEYAWRGFLQNIFFNKFGKKLGVI
                     ILGICWSLWHLPLIFTLYTPETPILGLILRSIHIVGISIFLGYLYIKTKNIWFCAIIH
                     ILNNSAFLITNSKESTITYHDILIVSMVVLIFYVPFLFTKEYKNNLE"
BASE COUNT        11292 a         2902 c         5195 g         8437 t
ORIGIN      
        1 taaagaaaat gttaaattaa taaataataa aaatatactt ttaatagatg atatatttac
       61 tactggtcta acaacaaacg aaatttctaa acttttaaag ttatcaggtg ctaataaggt
      121 ttttgtattg actctactta caaaatcaaa tgataattat attatggaat aatcttatat
      181 attgacaatt ttaatttaaa atattaaaat gaataagtga tatttaggtc tgtggttgaa
      241 agtccaagcc agtcgcaggc aaagggatcc acgtaagtta acactaaaaa ttgggttaat
      301 gatcatggtg cggcttagaa gtaagaccta ccgataataa aatcgagaga gagtagtagt
      361 gcggcgaacc caagcgaacc ctccagtagg cgagtgtggg gtcaaaaacc aggtcagcta
      421 aatatcgtat taaaagggag ctagcatacc tttatgtata gctctttttt atatataata
      481 aatttaggct gagtcataaa gaggtgatat gtttgaataa aagacaggaa aatttattaa
      541 caacgttgct attaaaagaa gagtttataa atgttaataa gattgccaat gattttgagt
      601 gttcagaaag gacaataaga aatgattgca aatatattga tgaatggtta tctacttttt
      661 gtaatgctta tatcaaaaga aaacctaata ttggaattaa atttagtgga gatggaacag
      721 acaaaatact ggtaaatcag aagttgcgag gtgaagttaa aagagtaaat agtgagcttc
      781 ataagcaagt tgagatatta aatttactac tttgtaataa taaaaaagtt actttaaatg
      841 atttatctaa taagttgtac ataaataaaa atattgtcag agaagaaata agtaaattat
      901 cagtggcatt aaataattat aatttaaatg tctcgactaa aaaaggcagt ggtatatata
      961 ttgaagggga tgagaaagac ataagaataa tgttagtatc attcttattt aaatacatag
     1021 ataaatacag attaaacata gatgaaatag agtacttcca tatagtagat ataataattg
     1081 taaaaaacat aatgtttact atagaggagg atatggatat aagacttaca gatatatcat
     1141 tcaagcaatt gatgctattt ttgcttataa atataatacg aataaggcta ggaaattctt
     1201 taaaagtatc aaatactaag aaagagctta agctaaaaat gcttatatta gatatagaag
     1261 atgaattgaa atctaattta tccatagtat taaattatga agaaaaattg tttatagaat
     1321 ctttaatatt gggtatgaac aaacaaataa atagtagtaa tattaagaaa tctttatatc
     1381 taaatgaaga attagcaagt tatacaaaaa aaattataaa tttagtatca gaagaatccg
     1441 gaataaattt taataatgat atattacttt atgaacaatt aatatgtcat ttgaatgtta
     1501 caatgcacca aatgaaaagt aatgtttact tagaaaatcc acttttagat aacataaaaa
     1561 ctaaattttg ttttttattt acagtaatat caagttcaat agaaattgaa ttaaaaggaa
     1621 aacaaataac agaagatgaa attggttatc ttacacttca ctttcaaacc tctctagaaa
     1681 gaaaatacag caaaagggaa tcaaataaaa aggcaagtat tgtatgtcca tttagttttg
     1741 gagtaagcat gctattaaaa gtaaagatag aaaagagatt tgataacata aaaattattg
     1801 aaacattaag agaggaagat ttaaaaagag ataacttcaa taagactata gattttataa
     1861 ttgcatttca agagtatgaa aatatccaaa aaccaatttt tataacaaca ccattattta
     1921 ctgatgagga tgagaataaa cttaaagagt tcacaaacaa aataaaagaa aaagacactt
     1981 catataaact tatgaacaga ttaatgatgt ctgattattt aataagagag ttagaacatg
     2041 atgatattta tgaagctata acatatttaa ctaatttatt aattgaaaaa cattatgcag
     2101 aaaaagagta tttacaatct attataacaa gagaaaagtc atatccaacc aatgttggaa
     2161 aaggaatatt attccctcat ggagatatga aatatataaa acaatctgtt ttatgttttg
     2221 caaggttaaa aacaccaata agaattagaa atggaaagga tataaagatt atacttttgt
     2281 tagcatataa aaaagatgat gatagaaaag tatttagaga attatttaaa gaaatatcta
     2341 atttaacaga ggatgaaaat atgctagata ttctaagtaa atgtgacatt gaaaaagtaa
     2401 aagatattct tatataaaat tcatagtgaa ctatggataa gcacaaaatt ttccatgtga
     2461 tggaaaaagt tgtgcatgtt tttttgtttt tataagatat tatatgtttg taaggtataa
     2521 ataatctaaa agtagagaag caacttagga ggaaaaaatg gaagaaatag ttttaaagat
     2581 tataattcat gctggaaatg caaaatctat gctttatgaa gcacttgact atgcaaagga
     2641 aaacgatttt aaaaaagcag atgaattaat tgaaaatgca aatgaagaaa ttttaaaagc
     2701 acataaagta caaactgaac ttatacaaaa ggaagctggg ggagataaat cagatatatc
     2761 tattttattg attcactctc aagaccattt gatgacttgt atgagtgaga gaaaccttat
     2821 aaaagaaatg attatgttaa gaaaagaaat tcaaaaatta aaataaaaaa attaaggggg
     2881 atttaaaaaa tgaaaagaaa agtatattta ttttgtagct ttggtatgtc aacaagttta
     2941 cttgcagata aaatgcagaa ggtagcagat gaacacaatt taccaataga agtagaggca
     3001 tttccgatag cagaaattga caaaatagtt gaagaaaaaa atccagattg tatattacta
     3061 ggaccacaag ttaaatacat gctaaaggaa ttaaaaccaa agtttgaagc tcaaggaaaa
     3121 cttatagata taatcaatga agttgattat ggaactatga atggggaaaa agtattaaag
     3181 ctcgctataa agctaataaa atccaaacaa gcgtagaatt atagtaaaaa ataataggaa
     3241 atgagggtag atagatgaat agattggaaa gaatacttat gccattggct gaaaaaatag
     3301 gtaaaaataa attattaatt gcaattagag atggtttttt aatatcatca ccattattaa
     3361 taataggttc attattttta ttagtagcaa attttcctat aaaaggatgg aatgaatttg
     3421 taggtcaaat ttttggttca gattgggctg taaaattgca acaaccaact gtagcaacat
     3481 ttgaagtaat gactctactt gcagttttag gaattggata ttcttatgca aagcaaatag
     3541 atgtagaccc aatagcatca gcagcagttg caatagttgc cttttttata ataacaccat
     3601 ttgtaatacc atatacacca gaaggaacag aatccgttta catggtaaca ggtataccac
     3661 taggttggat gggttctaag ggaatgtttg taggtatgat aacagcaata acctctgtaa
     3721 aattatttgc tgcagttgta aaaaaaggat ggacattgaa aatgccagat ggagttcctc
     3781 caactgtatc aaaatcattt gcagcattaa taccaagtgc aatagttatg gtagttttct
     3841 ttttagttaa gatagctttt gaagctacac catatggaaa tgtacatgaa tttatattta
     3901 attttttaca aataccgtta ttaaaattag gaaatacttt aagcgctaca gcaattgctt
     3961 atgtattctt acatctattc tggttctttg gaataaatgg aagtagtgtt gttggagctg
     4021 tgtataatcc aatattaaaa attctatcag ctgaaaactt agctgctttt caagctggta
     4081 caaaactacc aaacataata actgctcaat ttcaagatat gtttgcaaca tttggtgggg
     4141 caggtagtac attatcatta attatagcta tgttattaat atgtaaaagt aaacgtataa
     4201 aatcaatagg aaaattatca attttaccag gtatctttgg tataaatgaa ccattgatat
     4261 ttggtctacc aataatgctt aatccattac tattgatacc atttgcatta gttccaacag
     4321 taaatataat aatagcttat ttctgtatgt caacaggatt agtaccactt acaaatggag
     4381 ttcagctacc atggacaact ccaattatat tctctggatt tctaacaacg ggatggcaag
     4441 gggcagtttt acaattagta cttcttatac taggaatatt tatgtatata ccatttataa
     4501 aaatactaga caaacaatat ttgaaagacg aaaaatcaaa tgttgaagaa gaagatgatg
     4561 atatatcttt tgatgattta gtactatagg aggaagatta tgaagaaaat agtgatgata
     4621 ttggaccaaa tacaagctgg agctggagga aaagagaaaa gtaatatacc tcctgctgga
     4681 aaatcaagcc cattaggacc aggtgtaatg atggagcaat ttttaaatga atctaaggta
     4741 atagctacct tattttgtgg ggatgaattt tttgtaaata atcaagaaga agtaacgagt
     4801 aagatgatag ctatggtaaa aaaattaaac cctgatgtag taatctgtgg accaagcttt
     4861 aattacgaaa acttttctaa aatgtcagca atactgtcta aaaatataaa tgataaaaca
     4921 gatataccag cttttgcagc tatgtcagaa gaaaatatag atgtgataaa cgaatataaa
     4981 aatgacatat gtatagttaa gactccaaaa aaaggtggaa ttggtcttaa tgattcgtta
     5041 aataacatat gtaaattagc gaaagcaatg gcaaataaag aagatataac tttgatgaaa
     5101 gaagaatttt gttattagta caagtattaa ttattagcaa gtttgaatat tacactattt
     5161 ataataagca tacattagta aaatctatat aatttaagga ggggaatttt aacatgtggg
     5221 gaatgatagc gacatggaga atggccctag aaggaataac tgaggcatca gaacatttag
     5281 aaaaaggcat ggaagcaggt gatgctgtgg aacttgctgt aagaagagtt gaagattatc
     5341 cattctataa atcagttgga tatggtggac ttccaaatga aaattgtgaa gtagaacttg
     5401 acgctgcatt tatggatggt aacacattag atattggcgc agttgctggt ataagagatt
     5461 attctaatcc tgtcagcata gctaggaagt taagttatga gaaggtaaat aattttttag
     5521 ttggaatagg tgctgaagag tatgctcata aaaatggatt tgaaagaaaa aatatgttga
     5581 ctgatagagc gaagttacat tataaaaaga gaaaaaaaga gactttagac aaaggattaa
     5641 gcccatatgc tggtcatgat actgttggaa tgatatcttt agacaaaacc ggaaaaatgt
     5701 gtgcagctac atcaacaagt ggattgttta tgaaaaaaag aggtagaata ggagattctg
     5761 cactatctgg ttcaggattc tatgttgata gtagtatagg aggagctaca gctactggtt
     5821 tgggagaaga tttaatgaag ggatgtatat cttatgagat agttagactt atgaaagaag
     5881 gaaagcatcc acaagaagca tgtgatattg cagtaaatga acttgataaa cagcttatca
     5941 aaagaagagg aaaagctggt gatttatcat tagttgccat gaataataaa ggtgaatggg
     6001 gagtagctac aaatatagct gatttcactt tttcagtagt aactaaagaa gaatctccta
     6061 ctgtatatat ttcaaataga gtagatggaa agactataca taaggttata tcaaatgagt
     6121 ggatggaagc atatgaggct agaataaaag caccaataga agtaagtgag taaagtagtg
     6181 gttacataat taaaatagag ctttataaaa tagagtttta taataaggag atttttatga
     6241 atatcataag taataaaata gatgagttaa aagaagattt attaagtgat attatagata
     6301 ttgtaaaaat acctagtgtc agaggggagt cagaaaatgg cttccctttt ggcgaaaaag
     6361 taggggatgc acttaataaa gcattagaaa tatcagaaaa attgggattt aaggttacaa
     6421 atttggataa ttatattggt tatgcagaat atggagatag cgaagattat gtttgtgtaa
     6481 ttggacatgt agatgttgta catgaaggag aaggttggaa gtatcatcct tataagggag
     6541 aagaaactaa tggaagaatt tatggaagag gagtgctaga caataaaggt cctataatgt
     6601 ctgctttata tggattatat gctattaaag aattaaattt aaaattagat aaaagagtta
     6661 gaataatatt tggtacaaat gaagaaagtg gatttgaaga tataccatat tatttagaga
     6721 aagaaaaagc acctattatg ggatttacac cagattgtaa gtatcctgtt gtatatggtg
     6781 aaaaaggtat ggcaaagatt agaataaaaa gtaaaagcaa ttatgaagaa gagacattct
     6841 taggttttat agaaagcatg tctgaaaatg tgcttgttac atataaagag ttgaatatag
     6901 aaaatagtgg tattatattg gatataaagg taaaatacga ttttagttat aaattaaaag
     6961 atgtgttaga tgaaataaaa tctagttttc caaatagtat cgagatagag gtaatttcta
     7021 attttaatcc tgtatatttt gacaaagaga gtaatcttgt taaaaagctt cagctagctt
     7081 atgaaagggt tacatcatta gatggaactc cagtaacgac taatggaggt acatatgcaa
     7141 aagtaatgcc aaatatagtt ccatttggcc cttcatttcc aggacaaaaa ggaatagctc
     7201 ataatcctga tgaatatatg gatatagaag atataatatt aaatgcaaag atatttgcaa
     7261 atgcaatata tgaattggca aaggagtagt tatgttagag ataataggaa tgtcagcaga
     7321 agacgctaaa attatagaag attgtggtgc agatagaata gagttagtta gtgccttaac
     7381 tgaaggtgga ttgacaccaa gttttggtct tatagaaaag gttgttaaca gtgtaaagat
     7441 acctgtgaat gtaatgataa gacatcatgc caaaagtttt gtatatagta aagaagatat
     7501 aagtataatg caaaaagata taagtgtggt taaagagatt ggtgctaatg gagttgtgtt
     7561 tggagtattg gataaaaaca acaatataga tgaaaaaaat ctaaatgttt tattgaaatg
     7621 ttgtgataat ctcgatgtta catttcatag agctatagat gaatcaaata caatagatag
     7681 tgttaaaatt ttgaaggatt atgataaaat tacgaatata ttaacatcag gggggaaagg
     7741 aagtatagtt gataatatac aaatgataaa gaatatgatg cttaattcta atcacataaa
     7801 aattctttta ggtggaggtc ttaattttaa taatatagaa aagataaagg aattaactaa
     7861 ggcaagtaat tttcattttg gtacagctat tagaataaac aacagccctt ttgaagagat
     7921 agatagacaa aagcttaaac aactcgtaaa tataatttca agatagtata tgaatataaa
     7981 atacttagtt taaataaaga gagtaagtta attataattt gggtatatga atgttaatag
     8041 gtattaagta tgaaattatt gttgagtatg ctttataact tttaagtagt tttaaatata
     8101 aataatgaaa gaagagatag attatggagg ttttagatag cttatattta ttaaaaaatt
     8161 ataaaggtat attggcatat aatacatata atttcctaaa attaccatct tatgacatga
     8221 attcacatat taataaacaa ttaatggaga tttttgtgga tagaaatagc agtaaaaata
     8281 gctttaaata aacaaaaaca agagttatta acagttttat ccacattatc cacaggtgtt
     8341 tgtaataata ttaatccaca taagtatcaa tgtaactaaa aatgataaat aattctatca
     8401 attgaataga aaaaatcata aaagtaaata ataaaaaaag gattatgaga gttcgtgtag
     8461 aatatattta ttaaagtata aattttctta tactggcata attgaaggga gatgtactta
     8521 tgaacataat tatatctgga aaacaaatcg aattaactga tgcaataaaa aataaaatcg
     8581 aaactaaact aagtaagtta gataattaca ttcatccaga cactgatgta aaagttacag
     8641 taagtgcgag aaaagcaaga caaaaaatag aagtaacaat agctactgta aatggtccta
     8701 taattagagc agaagatatg caggaaaatt tatacacagc tatagatgtt gtatatgata
     8761 aattaagtaa acaattaaaa aaatataaaa agagattaca agataaacat aaagacaaca
     8821 agagtataag attccaagat gcagagataa atctagctga tgaacaagag gattatgggg
     8881 atgaaaatca agaattagta attcaaagac gtaagaaatt tagtgttaag ccaatgagtg
     8941 aagaagaagc tgtattacag atggaattaa taggacatga tttttatatg ttcaagaaca
     9001 tagattcaga tgaaatagca gtagtttata agcgtaataa tggtggatat ggtataatag
     9061 aacatgaata aagcaattta gatttaatca tattagctat cgataaataa attatcgata
     9121 gctttttaaa ttaatattaa aagtatagtt tttatggtaa aatgaataat agtacaataa
     9181 agaacgagga gagatgtaaa atatgagctt tatggataat ttattcaaca tggcagataa
     9241 aaaagaatta aaaaagttca ataaaactgt tgatataata gattcattag aacctaaatt
     9301 tgaatctatg gcagactcgg aacttaaaaa tatgacaaat atatttaaag aaagattagc
     9361 aaatggtgaa agtatagatg atatacttcc agaagctttt gcagtagtta gagaagtttc
     9421 taagagagtg ttaggactta gacactacag agttcaaatg attggaggta tagtactaca
     9481 tcaaggaaga atagcagaaa tgaaaacagg tgaaggtaaa actttggttg caactgctcc
     9541 tgtttatttg aatgcgctta ctggaaaagg tgtacatgtt gtaacagtaa atgattacct
     9601 tgcaaaacgt gatagagacc aaatggcaaa aatctatgag tttttaggaa tgagtgtagg
     9661 tgttattata cacggtcaaa atccaaaagt tagaaaagaa caatatgatt gtgatataac
     9721 ttatggtaca aataatgaat atggatttga ttatctaaag gacaatatgg taatacacaa
     9781 agagcaaaga gttcaaagag gactaaatta tgctatagtg gatgaggttg actctatact
     9841 aatagatgag gctagaacac cacttatcat atctggacct ggagacaaat caactcactt
     9901 atattcagat gcaaacacat ttgttttaac cttaaaacca gatgattatg aattagaaga
     9961 aaaagataag gctgtatctt taacagcatc gggtatacaa aaagcagaag tgtattttaa
    10021 tgttgataat ataacggata tatctcatac agaattatac catcatataa atcaagcttt
    10081 aagagcacat gtaataatga agaaagatgt tgattatgtt gctaaagatg gagaaatagt
    10141 aatagtagat gagtttacag gtagacttat gtttggtaga agatattcag aaggtttgca
    10201 tcaagcaata gaggctaaag aaggtcttaa aatacaaaga gaatctaaaa ctctagctac
    10261 tgtaacattc caaaactact ttagaatgta caaaaaactt tctggtatga ctggtacagc
    10321 aaaaacagaa gaagaagagt ttaaagcaat ctacaaaatg gatgttttcc aagtacctac
    10381 taataagctt atgataagag aagacttacc agattgtgta tacaaaagtg aaataggtaa
    10441 atttaatgct gtagcacaag aaataattga aaggcacaaa gtcaatcaac caatacttgt
    10501 aggtacagta tcaatagaga aatcagaatt attgtcacaa atactaaaga aaaagggcat
    10561 aaagcatgag gtattaaatg ctaagcatca tgataaagag gcagaaataa tagcacaagc
    10621 tggtagactt ggagctgtta caatagctac taatatggct ggtcgtggta cagatatagt
    10681 tctaggtgga aatccagact ttttaactaa gagagaaatg agaagaaatg gatttaaaga
    10741 agaaatagta aatagagtag atactccaat agaaggtata cctgtaaaag gtaatgaaat
    10801 tttatttgaa gctagagaag agtatgaaaa actatttgaa aagtttaagc aacaaacgca
    10861 agaagaacaa aaacaagttg tagaagccgg tggacttgct ataataggta cagaaagaca
    10921 cgaatctcga agaatagata accagttgag aggtcgtgct ggacgtcaag gtgacccagg
    10981 tagttcaaga ttctatatag gtcttgatga tgaccttatg agattgtttg gtagtgatag
    11041 aatatctggt attgtagata aaataggtct tgaagaagat atgcctatag aacatagaat
    11101 attatctaag tctattgaag gagcacaaaa gaaagtcgaa ggtaaaaact ttggaataag
    11161 aaagcatgta cttcaatatg atgatgttat gaataaacaa agagaaataa tatacgcaga
    11221 gagaaaacgt gtattagaag gtgaagattt acaagaacaa atacaatcaa tgactcattc
    11281 tataatagaa gaagcagtta ctctttatac acaagataaa ggatttgatg aagaaggatt
    11341 caaagaacat atgtataatc tgttcttgcc aaaaggtagc atagaaatac cagaaataga
    11401 aaaacttaat ccagttgaaa taacagaaaa agtttatgaa atagctatga agatatatac
    11461 ttcaaaagaa gaacaagttg gctatgagag aatgagagaa gttgagagag taatcttact
    11521 tcaagctgtt gataaccatt ggatagacca tatagatgct atggaccaat tacgccaagg
    11581 tattggtctt cgtgctgtag gtcagcaaga cccagttatt gcttataaaa tggaaggttt
    11641 tgatatgttt gatgaaatga ataaacatat caaagaagat actgtaagat atttatttaa
    11701 tataacaata gaaactccag tggaaagaaa agcagttgta gatgttgaaa atctatcttc
    11761 tccatcagat ggaacacttc caacaagtaa aactgttaaa aaagatgaaa aggttggaag
    11821 aaatgactta tgcccttgtg gaagtggtaa aaaatataaa aactgttgtg gaagataatc
    11881 tcggaggatt aaatgttaaa gttacaagag tatagacata gtatggagca gatggcttca
    11941 aatttagaag aattgagggt ttctctttga catagcttca ttgatgaaaa atatacaaat
    12001 taatgaagaa aagatgaata aacaagactt ttggaatgat aatgaagtcg ctcaaagagt
    12061 acttcaagaa aataagtctt taaaagagac attagaagaa tatgaaagct taaaaagctt
    12121 attagaagat atagaagtat taattgaaat aggtttagaa gaagatgatg attctgtaga
    12181 aagagatata gaaaaatcca tagaatcaat ggaagaaaaa ttatctgaaa tgaaaataaa
    12241 gactcttcta aatggagagt atgataaaaa taatgctata ttatctataa atgcgggtac
    12301 tggtggctta gatgctcaag attgggctca aatgcttttg agaatgtata taagatggtc
    12361 agaaagtaag ggttataaag ttaagctctt agatataata tcagaccctg aagcagggat
    12421 aaagacagct acaattcttg tagaaggaac aaatgcttat gggtatttaa agagtgaaaa
    12481 aggagttcat agattagtga gaatatctcc atttgaccca tcaggaaaaa gacatacttc
    12541 atttgcatct atagatgtaa caccagagtt agatgaaaat atagaagtag aaattaatcc
    12601 atcagattta aagatagaca catatagagc ttctggagct ggagggcaac atgtaaatac
    12661 aactgattca gcagttagaa taacacatat ccctactggt gtagtagttc agtgccaaaa
    12721 tgaaagatct cagcatctaa ataaggacag agctatgaga cttttaatgg caaaattgat
    12781 agaattaaaa gagttagaac aaaaggaaaa aatagaagat atacaaggca aatactccca
    12841 aataacttgg ggtagtcaaa ttaggtcata tgtttttcaa ccatataaat tggttaaaga
    12901 ccatagaaca aatgctgagt ttggaaatgt agatagtgta atgaatggga atatagactt
    12961 gtttattaat gaatacttaa aaatgaataa aatagtttaa taaatatgca cttacagcac
    13021 ttaggctgag gacgataacc ctcagccttc gttctgtttt gttatactta tgaaaaacta
    13081 acattaggag tgaataaatt ggatataaat caaattttaa aaaaagagtt taatcttaga
    13141 gatgaacaaa taaataatac attaaaactt attgatgaag gaaatacaat accatttata
    13201 gctagatata gaaaagaaat gactggcgaa atgagtgacg ttacactaag agaattttat
    13261 gaaaagttaa tgtatttgag aaatcttcaa agtagaaaag atgatgtagt tagactaata
    13321 gatgaacaag gtaaattaac agatgaaata acacaaaata tagaaaaagc aaaaacatta
    13381 caagaagtag aagatatata tgctccatat aaacaaaaga aaagaactag agcaacaata
    13441 gcaaaagaaa aaggattaga aaatttagca ttgtcaatac tagagaataa tttagataat
    13501 atagaaatag aggcaaagaa ttatcttgat gaagagaaag aagttctttc tattgaagat
    13561 gctctaaaag gtgctagaga tataattgca gaactagtct cagatgatgc taagattaga
    13621 aagtatataa gagaattagc tttaagagaa ggtatgatag ttagtaaaag tgccactgat
    13681 gaaaaatcgg tttatgacat gtattatgat tatagtgaag cagtaaaaag tatggctcca
    13741 catagagttt tggcaataaa cagaggagaa aaagaaaact tcctaaaggt taagttagaa
    13801 ataaataatg ataaagtttt aaattacata ataaacgaat atgtaaatga taaaaatttc
    13861 aaaaataaag aagaaatcgt aagttcaata gaagattctt ataaaagatt aattttccca
    13921 tcaatagaaa gagaaataag aaatcattta acagaaatag ctcaagaaag agctataagt
    13981 gtatttggaa agaatgttaa aagtttattg cttcaaccac cagttaaaga caaagtagtt
    14041 atgggatttg accctgcatt tagaactgga tgtaaaatag ctgtagtaga taaaaatggt
    14101 aaactactag attatacaac agtatatcca acagaccctc aaaatgatgt agagggagca
    14161 aagaaagttt taaaaggatt gattgaaaaa tacgatatag atataatttc aattggaaat
    14221 ggtactgcat ctagagaatc agaaactttt gtatcagaaa tgataaaaga aatagatagc
    14281 gaagtgcagt atgtgatagt tagtgaagca ggagcatctg tttattcagc ctctgaactt
    14341 gcaaatgagg aacatccaga tataaatgta tcaataagag gagctatatc tatagctaga
    14401 agattacaag acccattagc agagcttgta aaaatagacc ctaaaagtat tggtgttggt
    14461 caatatcagc atgatttaaa caaaaagaga ttagaagaag tactagatgg agttgttgaa
    14521 gattctgtaa atagtgttgg agtagattta aatactgcat catactccct actagaacat
    14581 gttgcaggaa taagtaaagc tatagcaaaa aatataatag catatagaga agaaaatgga
    14641 gattttacgt caagagcaca attgaaaaaa gtaaaaagac taggaccaca agcatttact
    14701 caatgtgctg gatttatgag aatcttagag ggtaaaaatc cactagataa tacaggtgtg
    14761 catcctgaaa gttatgatat atgtaaaaaa atgattgaga taattggata ttctttagat
    14821 gatgtaaaga ataagaatat aggcgaaata gatgaaaaaa taaaagaaat tggcttgaga
    14881 gaattaagtg aaaaactgga agtagggcaa gttacgttaa aagatatcat aactgaaatc
    14941 aaaaaaccag gaagagaccc aagagaagaa ggtataaaac ctatacttag aactgatgtc
    15001 ttaaaaattg aagatataca agagggtatg actttaaaag gtacaataag aaatgttgta
    15061 gattttggtg catttgtaga tattggaata aaaaatgacg gattagttca taaatcagaa
    15121 atgagtaata gttttgtgaa agaccctatg agtattgtta cagtaggtga tatagtagat
    15181 gtaaaagtca ttggaattga cttaaacaaa aaaagagttg ccttatcaat gaaaaaatag
    15241 ataagtttat gtatggttat agtacttaaa ttctatgttt gtaaatgagg ttattcgagt
    15301 gtataaatgt taaaaatttg taaaagtata cagtaagaaa atatattaaa ataagaacga
    15361 tagtcttgaa aatcaggagg aaaatgaatg atttttataa aaaatggtaa aataaatacc
    15421 ataacaaatg ggataattca tggagatata cttatagatg aaggaaaaat aatagaaatt
    15481 ggagaagatt tgatagctcc tttagatgta gaggtaatag atgctagtaa taaacttgtg
    15541 tttcctggat ttatagatgc tcatactcat ttaggtttgt gggaagatgg tatagggttt
    15601 gaaggagcag atggaaatga agagactgac ccgataacac cacaattaaa tcccatagat
    15661 ggaattaatc caatggatag aacttttaaa gaagcttttg aaggtggaat aacttctgtt
    15721 tgtacaaccc ctggaagtgc aaatgttatg ggtggtcaat gtatagctat aaagacatgt
    15781 ggaaaaagaa ttgataaaat ggtaataaaa aatccagttg catctaaaat cgcttttgga
    15841 gaaaatccaa agagctgtta tggtcaggat gataaatctc ctcaaactag aatggctata
    15901 gctgctttac ttagagaaaa cctaaaaaaa gcagaagaat atttagagga tattgatatg
    15961 tatgagagtc atgatgatga agactgtgaa aaacctgaat atgatataaa aatggaaagt
    16021 ttaataccag ttttaagaag agaaattcct tttaaggcac atgctcatag agcagatgat
    16081 atgtttacag caataagaat tgcaaaagaa tttaatttaa agcttacatt agaccattgt
    16141 acagaaggtc atcttattgt agatgaatta gtggaagaag ggtttcctgt aatagtaggg
    16201 ccatctttgt cagaaaggtc taaatttgag cttagaaatt tgacatttaa tacagcagga
    16261 atactttcaa atgcaggtct tgatgtatgt ataatgacag accatccagt aataccagtt
    16321 caatatcttc caatatgtgc aggtattgca gtcaaacatg gtatgaaaga agaaaaagca
    16381 atagaatcaa ttacgataaa tccagctaag actttaggaa tagaagatag agtaggctct
    16441 atagaagttg gtaaagatgc agacctagta atttgggata attctccact agagattcaa
    16501 agtaatgtac tttacactat aataaatggc aaagtagtgt atgagaaaaa ataacttgac
    16561 aattattaaa aaattaacta atataagagt gtaaggaaaa ttaaaaaatc aaagaagatc
    16621 ttcggggcag ggtgaaattc cctaccggcg gtatagcccg cgaactactt aggtggttga
    16681 tttggtgaga ttccaaagcc gacagtaaag tctggatgag agaagatatt tatggaagat
    16741 aaataagttt attttagttt gtttaaacta tatccataaa aactcgaaga tagggtcttc
    16801 gagttttttg tttttcctaa aaaatataaa catattgatt agggggaaaa taattatgca
    16861 aaacacagcc aaaagaggca atatgatttc aacaagaact ttagtagtaa tgtcaatttt
    16921 atctgcaata tcttatttat taatgtttat tcaagcacca ataccaggta tatttccaga
    16981 tttcttaaaa atcgatttat cagatattcc agcaatattc ggaggaatgt ctctaggacc
    17041 attagttgga tttggaatag ttgtagtaaa aaatgtgtta caagcaataa cagcaacaac
    17101 aactggagga ataggagaat ttgcaaatat attaattggt ggttcttatg taatgatact
    17161 ttgttattca tataagagaa gtggagattt aaaaggtgta ctgattggtt ttgtattagg
    17221 aacaatatct atgactataa ttggttcatt agtaaattat tttgtaatgt tgccacttta
    17281 tggtcaactt atgggactag atgctataat aggtttaggt tcagctataa atcctcaagt
    17341 tcatgattta ttcacttttg taatttggat gatagctcca tttaatgtgc tgaaggctgt
    17401 tgtaatatct ttagtaacat tgccacttta taaaaaaatg ggaaatataa taaagaaata
    17461 attagacaag aaaatataaa attaaatgta ttgaatagcc ataatttatt tataacttca
    17521 gtcatatatt gtgattgata tattaaatat attatggctt tattaattta tcaaaaaatg
    17581 tatataaaat aagaaatatt tagacaaatt taaaatttaa aataaaggaa gaattaaaac
    17641 gattaactat taaaatatga aaaaggtatc tacaatatac agagcgaatt tataataaac
    17701 aaaaaataaa gttattatta aaaaactata aagattaata atcaaacttt taataataac
    17761 taatttaata atgatagggg tgtatattca tgtatttgtc taaaaaaata aaaaaattat
    17821 ttttagatga taaagggtat tattttaata gtaattggaa agatattcat attggtattt
    17881 atgatacaaa attaggagat tctttaaagt atatcactaa tgaatatatt gaaaatatgg
    17941 aaagggtaaa atacgattat cgtgaattaa aaaatataga aaatcaaact gatggtatgt
    18001 gtattttagc aataaggcag aattacaagg cattagagta tgtaaaaaat caaaccgaag
    18061 aattatgttt agaagcaata aagtataatt ataaagcact tgaatatgta aaagagcaaa
    18121 cagaatatct gtgtcttgaa gctataaaaa aggattgtaa tgcacttaag tatgtacgaa
    18181 ataaaacaga agggcttatt ataaaagcaa taagtcattc ttccaatata gatgttgtaa
    18241 gtatactaaa ggccttagag actcaaacaa gaagaatatg tttagaagca attaaaaaag
    18301 atggcaggtg tcttgcatac gttagagaac agagtgaaga attatgtata gaggccatta
    18361 agcaaaatta taaagcacta aaatatgtaa aaaatcaaac ggaaaaaatg tgtatagaat
    18421 ccgtaagaca aaatggtatg gcattacaat atgtaaataa acaaactgat aaaatatgta
    18481 tagaagctgt caaacaagat ggaagaagtc tacaatttgt caataataag acagaagaaa
    18541 tatgtataaa tgctataaga tatttaaata aaaaatataa tattaaagat gtactaagtt
    18601 acatagataa gtatacagaa gatatatgta tagagattgt aagacaaaat ggaaaaatgc
    18661 ttatgtatat aaaaaatcaa acagaaaaaa tgtgtattga agcagtaaaa gagaactata
    18721 agtctcttaa gtatgtaaaa gagcaatctg agagaatatg taaagaagca ttgaagcaaa
    18781 accataaagc aaaagagtat gtaaaaattg ctattgatga ttgtatataa ggtgtagata
    18841 cagccatcaa tagtgaaaga taaaaaaaga taaagacaag tactttataa taatctaggc
    18901 ttatactata attaattaag cagtgatata aattcacatg acgattatat caaaatggag
    18961 atgaattgat ggcaaaaata tatttagaaa atgaaaataa aactagagaa ataggatata
    19021 aattaggtaa acttttaaaa gaaggcagtg ttatctgttt agtaggagat ttaggagctg
    19081 gtaaaactac aatgacacaa agcttggcag attcattagg aatagaagat tatataacaa
    19141 gtcctacttt tactataata aatgaatatg aaggaaaaat acctttgtat cattttgatg
    19201 tttatagaat aggtagtagt gatgaaatgt atgatattgg ttatgatgaa tatgtaaatt
    19261 caaatggaat ctgtataata gaatgggcta atctaataga agatatactt ccaaaagaat
    19321 atttgaatat agaattgaaa tataaagatg aaggaagaga gatgatatta actccaaaag
    19381 gagagtttta taaagagata gttgaggagt tgattaaata atgaaaatac taggtatgga
    19441 tacatcttct atggcagcaa gtgtagcagt tgttgaagat aataacttaa tatgtgagtt
    19501 tacagtaaac aacaaaaaaa ctcattcaca aaagcttatg ccaatgatag aaaatatgtt
    19561 gagtatgagt gatttaagta taaaagatat ggatttatta gctgtatgta taggcccagg
    19621 ttcgtttaca ggacttagaa taggtatggc tacagtaaaa gctatggctc atgtaaataa
    19681 tataccaata atagcagtaa attctttaga atctctagca aataacataa atttctgtga
    19741 tagaaagata tgttgtatat tagatgcgca gaaaaatcaa gtttattcag ctaaatacaa
    19801 gtttgaaaat ggagagatga tagaattaga tggtgttgat gttgtagagt ttgatgcttt
    19861 agtagaggaa attgtatcaa ctaatgaaga atttataata gttggagaag ctgtatacaa
    19921 atataaagac aaattagaga atatagaaaa tataaaaata ccttcaccag ctaataatgt
    19981 atctaacgca ggaagtttat gctctttagc gttaaataaa tatagtaaaa atatagatgt
    20041 acatacttgt tatacgataa atcctatgta tatcagaaaa tctcaagctg aagtacaata
    20101 tgatgaaaaa atgaagaggc taaataatgg aagataatat aaaactaata gtagatgata
    20161 taaaaataga agaaatgact acagaagata tagatgaagt ttttgaagta gaaaaaaatt
    20221 gttttgaaga ttattggtca aaagagtctt ttagaaaaga gttgtctaat gaagtagcaa
    20281 ggtatatagt agctaagtta aatggaaaaa ttgttggata tgtgggaatt tggcttatac
    20341 ttgatgaagg tcatataaat aatgtggctg ttcatagtga ctatagaggg aaaaaaatag
    20401 gggacaagtt gataaaaggc atagttgatt tatgtaaaga taataatatt gcatctatga
    20461 cattagaagt tagagcatca aataaaatag cacaaaattt atatagaaaa tatggattta
    20521 aaatgggtgg aataagaaaa gaatactaca gtgataataa agaagatgca attataatgt
    20581 ggaaccaatt aaaagaggtg tagagtttat gagtgatata ataacattag ctatagagag
    20641 tagttgtgac gaaacagctg catcagtttt aaaaaatggt agagaagtac tttcaaacat
    20701 aatctctacc caaatagaga cacataaaaa atttggtggt gtagttccag aagtggcttc
    20761 aagaaaacat gtggaaaata ttgatatagt tgttcaagaa gctttagaca aagcaaatat
    20821 aggttttaat gatatagacc atattgcagt tacatatgga cctggactag taggtgcttt
    20881 attggttggt ctatcttatg ctaaagcact tgcatatact ttgaatattc cacttgtagg
    20941 agtaaatcat atagaagggc atctaagtgc aaactatata gagcataagg atttaaaacc
    21001 tccttttata acattaatag tatctggagg tcatactcac ttggttgaag taaaagatta
    21061 tggcaagtat gaaattttag gaaaaacaag agatgatgca tctggagaag catttgataa
    21121 aatttcaaga gcaatgaatt taggttatcc aggaggacct attattgata atctagccaa
    21181 gaagggaaat aaacatgcta tagagtttcc aagagcctat ttagaagaag atagttatga
    21241 ttttagtttt agtggattaa aatcttctgt attgaattat ctaaatggga aaagaatgaa
    21301 gaatgaagaa atagtagttg aagatgtagc agcatctttt caagaggctg tggtagaggt
    21361 tttatctact aaggcactaa aagcagtaaa agaaaaggga tacaatatca taactctttc
    21421 tggtggagtt gcttctaatt cggggcttag agctaaaata acagagcttg ctaaagacag
    21481 tggtattact gttaagtatc ctcctctaat tttatgcaca gataatgcag ctatgatagg
    21541 gtgtgcagga tattataatt ttataaacgg gaaaacgcat gatatgagtt taaatgcagt
    21601 tccaaattta aagataaatc agtagagtta gataaatttg taaatataaa agtattgttt
    21661 caaaaaagta agcagaagtg aaattaaata taataaaaat caatatagta tatattaaaa
    21721 cttgaagtaa aactatttat aatctttata ttccaatgaa ttgcaacaca ttttaggata
    21781 tacaggttgt ctttggctta gatttgcttc aagtttaatt ttttctatag aaagattatt
    21841 ttaaaaagtg ggtaatatac cctttttttt cattcttaaa aagttttata ctaattatag
    21901 aaagattaag gaggggaaga aatgagtcaa agtaaagaag acaaaataag aagtattttg
    21961 gaagcaaaaa atatcaaatc aaattttcaa aataaagaaa atttaagtga atttaatgaa
    22021 aaaaaagcaa gtaaaagagc agaagactta ttagatgtat attataatac attatctact
    22081 gcagatatgg aatttccata ttggtataat agagaatata gaaaatcaga tggtgatata
    22141 ccagtagtta gaagagctaa agctcttaaa gctgcttttt cacatatgac accaaatata
    22201 atacctggtg agaaaatagt aatgcaaaag acacgccatt atagaggttc ttttccaatg
    22261 ccatgggtaa gtgaaagttt ctttgttgct caaggagaac aaatgagaga agaggcaaag
    22321 aaattagcta gtaacacagc tgatgaactt acaaaatttg ggtctggtgg aggaaatgtt
    22381 actgaaagtt ttggaaatgt cgtttcaata gctggtaaat tcggaatgag aaaagaagaa
    22441 gtacctgtac ttgtaaaaat ggcaaaagaa tgggttggaa agtctgttga agatttaggt
    22501 tttcactatg aaaaaatgat gccagattat gatttaaaag aaaatctaat gagtacactt
    22561 atatgtatgt ttgattcagg atatacactt cctcaaggta gagaagttat aaattatttt
    22621 tatcctttaa attatggact agatggaatt atagaaatgg caaaagaatg taaaaaggca
    22681 gttgcaggaa atgcatctgg agatgggctt attggtatgg accgtctata tttttatgag
    22741 gctgttattc aagttattga aggattacaa acttggattt taaattatgc aaaacatgca
    22801 aaatatttag aaagtataga gacagattta gaagctaaaa aagaatactc agatttagtg
    22861 gagatattag aacatattgt acataaacaa cctcgtacat ttagggaagc tcttcagttg
    22921 acttatacaa ttcatatagc atcagtaaat gaggatgcta tatctggtat gtctataggc
    22981 cgttttggtc aaattttata tccttggtat gaacaagata tagaaaaagg attaattaca
    23041 aaagaagagg ttatcgaatt attagagctt tatagaataa aaatcacttg tattgactgt
    23101 tttgcttcag cgggcgtaaa tggaggagta ctatcaggaa atacatttaa tactttatct
    23161 ataggtggat taaaagaaga tggttctaca ggagctaatg aactagagga gctattatta
    23221 gaagcaagta tgagatgtag aactcctcaa cctagtttaa ctatgttata tgatgaaaaa
    23281 attccagaag atttcttaat gaaagctgct gaatgtacaa agcttggttc tggttatcca
    23341 gcctgggtaa acaactcaaa tggaacaaca tttatgatga agcaatttgc tgatgaaggt
    23401 atgactgttg aagaagcgag agcatttgca ttaggaggat gtttggaaac gtctccaggg
    23461 tgctggaaac agttaacttt aaacgggaag acatactcta ttgcaggtgg agcaggtcaa
    23521 tctgctggct ctggagttca ctttatagct aatcccaaaa ttttagaact tgttcttatg
    23581 aatggtaaag accatcgtat gaatatacaa gtatttgagc ctcataataa gccattagat
    23641 acttatgaag aagtcataga agtatttaaa gattattata aacaagcaat aaatgtttta
    23701 gaaagagcaa ataacattga gttagatata tggagaaaat ttgatacttc tgtaataaat
    23761 tcactattaa agccagactg tttagacaaa ggtcaacata taggaaatat gggatatcgt
    23821 tataatgcaa cattaaacgt agaaacttgt ggtactgtta ctatggtcaa ttcatttgca
    23881 gcacttaaga aacttgtata tgatgacaaa gcatttacaa tagaggaaat gaaggatgcc
    23941 atattaaata attttggatt taaagatgca ttagaagtag gaaattattc tatggcagac
    24001 caagtaaaag tagataagac tggtaaatat gatgctattt ataaggcctg tcttgatgca
    24061 cctaaatatg gaaataatga tttgtatgca gataatattc ttaaaaacta tgaggtatgg
    24121 ttatcaaaag tatgtgaaga agcacaatct ttatatgcta agaagatgta cccttgccaa
    24181 atatctgttt ctacacatgg accacaggga gctgctacct tggctactcc agatggtaga
    24241 ctaagtggaa caacatattc agatggttca gtctctgcat atgcaggaac agataaaaat
    24301 ggagtatatg cattatttga atctgcaact atttgggacc aagcagtagt tcaaaattct
    24361 cagatgaatt taaaacttca tccaacaact ataaaagggc aacaaggaac taaaaaatta
    24421 ttagatttaa caagaagtta tttaagaaaa ggtgggttcc atattcaata taatgttgta
    24481 gattcagaaa cattaaaaga tgcacaaaaa aatcctgaca attatcgtca attaatggta
    24541 cgtgttgctg ggtttactca atattggtgt gaattaggta aaccaataca agatgaagta
    24601 attgctagaa cagagtatga aggggtgtaa aatatgagaa agcatagtga ttgtatgaat
    24661 ttttgtgcag tagacgcaac caaaggaatt tgtagattat caaaacaaat gattaattta
    24721 gatgacgcag catgtccaga gataaaagta atgccaaaat gtaaaaattg taaaaatttt
    24781 gttgaagcta atgatgaagg tataggcaaa tgtgttggtc tagagaaaga agactgggta
    24841 tattcaacat tgaatgcaat tacttgtgaa ggacatgtgt ttaatgagta gtcaaaagca
    24901 attagaaggc atgattttcg atgtacaaag cttttcagtt catgatggtc caggttgtag
    24961 aacaactgta tttttaaatg gatgtccact gagttgtaaa tggtgtgcaa atccagaaag
    25021 ttggactgta agaccacata tgatgtttag tgaattatct tgtcaatatg aaaatggatg
    25081 tactgtatgt catggtaagt gtaaaaatgg tgcattgagc tttaatcttg ataataagcc
    25141 agttatagat tggaacatct gtaaggactg tgaaagtttt gaatgcgtta actcatgtta
    25201 ttataatgcg tttaaattat gtgcaaaacc ttatactgta gatgaacttg tgcaggtaat
    25261 taaacgtgat tctaacaatt ggagaagtaa tggaggagta acatttagtg gaggtgagcc
    25321 actacttcag catgaatttt tacatgaagt attattaaag tgtcatgagg taaatataca
    25381 cactgccatt gaaacgagtg catgtgtatc aaatgaagtt tttaataaaa tatttaaaga
    25441 tattgatttt gcatttattg atataaagca tatggataga gaaaaacaca aagaacaaac
    25501 aggtgtgtat aatgatttaa tattagaaaa tatatcaaac cttgcaaatt ctgattggaa
    25561 tggaagatta gtacttagag taccagttat ctctgggttt aatgatagtg ctgaaaatat
    25621 aagtgatatt atatctttta tgcataaaaa taatttggta gaaattaatc tattgccatt
    25681 tcacagatta ggagaatcaa aatggattca attaggcaag gaatatgagt attctgataa
    25741 gggtgatgtt gatgaaggtc atcttgaaga attacaagat atgtttttgg ataatggtat
    25801 agcttgttat gttggtcatg agacagcttt ctaaaatcaa atacaagttt taattaaaaa
    25861 aggatgttac attaaaagct agagttacat ccttttttat gtgggtttta tgtagaaata
    25921 attaaataaa gttttatata ttgtttatat tgatttatgt tcataaatat tgattcaaat
    25981 ttatacctta tatatttttt atagtttaaa ttacagttag agaatatatt acgtttactt
    26041 tattgaagca gaagttttgt ggtaaaatac tactatgaaa aaatttaata gaatatattt
    26101 tataaataac taaaaataat agttagtagg aggtcatcta tatggacaaa tcaaggagca
    26161 acttgattat taagaacaaa gacaaaaaag tacgctcagg gtggattatt atagctgtta
    26221 tggctacctt ttatatacta cagtattatt caagcactct acttatggaa atactaagaa
    26281 aaattttgat tgctagaggt gacattaatc taaaaacaaa ctacttttcg gaatatgcta
    26341 attggttaaa tgactttggg ttaccgattg tatttcaaat tctaacagat gcacttatga
    26401 ttactattcc gataattgta tggagattta ttatgaaaaa tcctattagt gaaatgggat
    26461 tacattctta caagattaag aagaaagaag gatatgtagg aatgttgtta ggaatattta
    26521 actgtacagt aatctttatt ataacaataa caattggtgg tggtcatgtt atttcatggg
    26581 taccaaaaat ttcaccttta acagtttctt ggatacttgt ctttatttta attgcttttg
    26641 cagaagaaat tttaaatcgt ggttttttta tggcagtttt acgaagatgt aaaaatattt
    26701 attttataat gattgttcca tctgttattt ttgggttgat tcatatatgg aatcctgatg
    26761 ttactttttt atctgtaatt aatattataa ttgttgggat tcttttttca tatatgttta
    26821 taaaatcgtc gaatatatgg atgtgtatag gttatcattt tgcatggaat gtatttcagg
    26881 ggattatata tggtatgcct gtaagtggac ttcaagttcc agggattata actacacaaa
    26941 tcactcatgg taatctatta aatggaggaa tgtttggtat tgaaggtgga attttgacta
    27001 cttttgttaa tctacttagc tttatatttg tatggtatta ctatagagat tctaagtatg
    27061 attttgttag taataagtaa gtgtgaataa tgttaagaac taattaatta taataaattt
    27121 taataatata ttctaggctt aaagtattct gtaggattgt tggaaattta caaaaatata
    27181 aaaataactt ggatattcta tttactccaa gttattttta tattcttttg taaatagaaa
    27241 tggaacataa aaaattaaaa caaccataga tactattagt atatcatggt aagtgatagt
    27301 tgattcttta gaatttgtga taagaaaagc actattattt aaaatatgaa ttatagcaca
    27361 aaaccagatg ttcttagttt ttatataaag atatcctaaa aatattgaaa ttccaactat
    27421 atgtatcgaa cgtaatatta atcctaatat tggtgtttca ggtgtatata atgtaaatat
    27481 tagtggtaag tgccacaaac tccaacaaat acctaatatt ataactccta gttttttacc
    27541 aaatttatta aaaaaaatat tctgtaaaaa acctctccag gcatattctt cactaaaaaa
    27601 aatataaata gtagttaatg atgttattgg agtaaatata aattttatga taccactata
    27661 attaggttga gccccttgta gtattaatat aaatgatgtt ggaacaatta cagaaatcat
    27721 aaagtaaata attaacaaag aacctagttt aatatttttt acaggatata aatttttttt
    27781 atttgatgaa tatattataa ttgataaact acttataaaa gttaat
//
LOCUS       DAEPXF010000034        27408 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain HB27
            SAMN13376389-rid12479423.denovo.036, whole genome shotgun sequence.
ACCESSION   DAEPXF010000034 DAEPXF010000000
VERSION     DAEPXF010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13376389
            Sequence Read Archive: SRR10518432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 27408)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27408)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001069624.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001069624.1
            Genome Coverage       :: 315x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/15/2021 01:54:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,165
            CDSs (total)                      :: 4,076
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 89
            rRNAs                             :: 10, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 10 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 19 of 69
            Pseudo Genes (incomplete)         :: 44 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 10 of 69
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27408
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13376389-rid12479423.denovo.036"
                     /strain="HB27"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="China: Hebei"
                     /collection_date="2017"
     gene            236..1141
                     /locus_tag="KRL67_001301"
     CDS             236..1141
                     /locus_tag="KRL67_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBH1522197.1"
                     /translation="MDFKQLEVFVAVAKHQSFSKAARELFLTQPTVSAHIQNLERELE
                     TVLINRSNKVITLTKSGEILYEHAIYILNNCKRAIYDIKEYSGKIEGIIDIACSSIPE
                     TYILPDFIKSFSMSYPDVKFSISHYDSQYAISEILNERISFGLVGSKINNPQIEYLDL
                     LDDELVLITPSDFKIDNKNNCIDIGELAYLNFIMRKEGSGTRNLILNTLSKNNFPVSK
                     LNVIAHVESNEAIKEMVRLGLGVSFISYISAIDYLNAGKIKCYKIKDVDFTRKFFFIY
                     SKKKTFSPLEDKFLNRLCEYFEIII"
     gene            complement(1301..2143)
                     /locus_tag="KRL67_001302"
     CDS             complement(1301..2143)
                     /locus_tag="KRL67_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HBH1522198.1"
                     /translation="MTTSSKKLETISPDSKITKKDFWKCFRRSLTLDSSWNYERMQNI
                     AYAYMMAPIIRRLYKDDKEKKSKALKRHLEFMSVTPHISTLLVGISGAMEEENAKNKE
                     FDANSINAVKSSLMGPVSGIGDSFFWGTLKLIAAGVGIALASQGNIMGPILFLLIINV
                     PHFIIRYICLDKGFKYGTQFFKDVSGSSIVSKVMEAASMLGLMVIGGMTASNVMLKLS
                     VNVGSGEWAEPIQTYLDQIMPCMLPAMIFGIMYWLLGKKVKTTTILISVMIICIVLAA
                     IGVV"
     gene            complement(2127..2885)
                     /locus_tag="KRL67_001303"
     CDS             complement(2127..2885)
                     /locus_tag="KRL67_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBH1522199.1"
                     /translation="MEFTQVILITLIAFFAYMHSFVGSTMHNRPIVVAPLVGLALGNL
                     HTGIVIGSTLELVFMGAFPVGASNPPDFVSGSIIATAFVILTGQDVSAAVVLAVPIAT
                     LVLLIDNFLMTVVLTWGAHIADRYAEEGNIEGVERVQLLFGIGNKLILAIIVGIGFSL
                     GVPVIEKILSFIPSYVTHGMDVAAGVIPAIGFAMLARMMLNKKTVAFLLLGFILVAYL
                     NITVTGVALFGLAIALIYVNFIGEKEVIVDDNEF"
     gene            complement(2902..3372)
                     /locus_tag="KRL67_001304"
     CDS             complement(2902..3372)
                     /locus_tag="KRL67_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBH1522200.1"
                     /translation="MIKLVRVDHRLIHGQVAFTWTKFLSTDCILIASDDLLKDELRMA
                     GLKMAKPSNVKLVMKSIADSIKALNSGVTDKYNLLILCESVEDVYRLAKEVKAIKSIN
                     LGGTKSDDNRENISKAVHVSKDDIKMIKELDSEGVNVFVQLVPDDDATNVMKLI"
     gene            complement(3372..4115)
                     /locus_tag="KRL67_001305"
     CDS             complement(3372..4115)
                     /locus_tag="KRL67_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane biogenesis protein"
                     /protein_id="HBH1522201.1"
                     /translation="MKKKGILSVFKSKKPIIAMIHLKGDTPEDIFERAKKEITIFEEN
                     GVDGIMLENYYGNYYDLERILEYVSKSNLSIPYGVNCLNVDTMGFELATKYNASYIQV
                     DSVVGHVKPRDEATLEEFFKLQRSKCPAYLIGGVRFKYQPVLSENDVEEDLKIGMTRC
                     DAIAVTENATGQETSMEKIELFRKNLGDFPLVIAAGVTLENAKKQLKLGDMAIIGSYF
                     KDNYKDFGDVSVEHVKTFMDEIKKIREEL"
     gene            complement(4119..4529)
                     /locus_tag="KRL67_001306"
     CDS             complement(4119..4529)
                     /locus_tag="KRL67_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBH1522202.1"
                     /translation="MKKIILASHGDFSKGLLNAVKMIVGDLADCVSSYGLYPGQSASD
                     FALELEKTISEDKECEYIILSDLYGASVCTAMLRLTNLHNVKLFSGMNLNMVLELLTR
                     FSDELTVEDIDQLVEESRRGIQSVTLQIKEEEVF"
     gene            complement(4555..5307)
                     /locus_tag="KRL67_001307"
     CDS             complement(4555..5307)
                     /locus_tag="KRL67_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBH1522203.1"
                     /translation="MDEKRQKKEMDLEVNSFVPLYQQLYDNIKKQIASGIYKPGDKLP
                     SEGDLCKEFNISRITVRNALNELVKEDILCKKRGKGTYVTIPERIEATCAGNSFTNSC
                     HRINAKPSTKIISVLIKKADKQVAEALSIELEEKVICIKRLRLIDEVPVIFEVDYFRI
                     DYMFLLKEELEDKSLMEVISNNISTLPKRVENIFEVKHSNKEYSDHLKCASNMPLLKV
                     RQSVYTENNDVLYYNEQFIRSDKYKYAVSAEI"
     gene            complement(5547..6941)
                     /locus_tag="KRL67_001308"
     CDS             complement(5547..6941)
                     /locus_tag="KRL67_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HBH1522204.1"
                     /translation="MNLKYEFKNAWDFERKENTIEQIMQYSRNYMEFLSKSKTERLSV
                     KEIIKLAKENNYISIDEAMEKGSISCGDKIYVINKEKAVALFVIGKNYIEKGMKIIGS
                     HIDSPRLDLKPNPLYQESNLGFFKTHYYGGIKKYQWTAVPLALHGIVILNDGTKVDIS
                     IGEEDSDPVFCVTDLLIHLAGDQMQKKLSEGISGEALNILIGNIPLEDEEKEPITANI
                     LKILNEKYNIVEEDLLSAEIEVVPAGKARDLGLDRSMVLGYGHDDRVCSYAAVKAILE
                     TEQPEFTSVALCVDKEEIGSKGNTGMHSKFFENTVAELIALEGDYCDIKVRRALANSK
                     VLSADVSAGYDPNFGEAYEKRNSAYMGNGVVLTKYTGSRGKSGCNDANAEFMSEVKRI
                     FNKGNVVWQTAELGKVDQGGGGTIAHILANQGAEVIDCGVGVLNMHAPHEIVSKVDIY
                     EMYKGYKAFFNINL"
     gene            complement(6961..7170)
                     /locus_tag="KRL67_001309"
     CDS             complement(6961..7170)
                     /locus_tag="KRL67_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF896 domain-containing protein"
                     /protein_id="HBH1522205.1"
                     /translation="MFDKKKLDRINELAKKNKEGILSADEIKEREILRKEYLENFRAH
                     FRSRLDSVKVVSPEEYEQYMKNNKN"
     gene            complement(7199..8644)
                     /locus_tag="KRL67_001310"
     CDS             complement(7199..8644)
                     /locus_tag="KRL67_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HBH1522206.1"
                     /translation="MEKYRIDLNEKKSTKYIQIFNHIKELIINKELVEHEKLPPIRKL
                     SNFLQVNNTTIVKVYELLENEGYVYKIIGSGTFVSSLNSYKSGIDNRKNKIIVQREDL
                     IHFDNGNPSNDMFPIDSFKNAVNMALSKDGSSIFEYDEGLGSEELREKMVEYLSDGNI
                     KTTKENIQIISGAQQGIDIVCKGLISYSDVVFMEEPSYNGAIEVFKSRGAKIISIPML
                     DDGIDIGILKLKLEKIKPRLIYIMPNFQNPTGISYSTYKKKKLIELAEEHDFYIIEDD
                     FISDFVFESKDNRTIRSYDDKNRVVYIKSFSKILMPGLRIGIVEMPNELLKKVLWAKY
                     SSDISTPGLIQKSMYYYMNNFDWKNYLSHIEKIYTDKYKLAKNLINDKLSGKLKVRKS
                     CGGINFFLELPRGYTSQVFTNFMLNKGVSMLPGTYFFDNLVDDRFFRINIARPSMEEL
                     EKGISIISDNIDEFFCEYKNKLDIKSNKLFY"
     gene            8754..9548
                     /locus_tag="KRL67_001311"
     CDS             8754..9548
                     /locus_tag="KRL67_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphatase HisJ family protein"
                     /protein_id="HBH1522207.1"
                     /translation="MFYDYHMHSSFSTDGKSTMEEMVKKSIELGLEEICFTDHVDYDV
                     YADDSFSIVYEDYFKSLETLQSKYKDKISIKKGIEFGVQTQLMDTYKKEAHQYPLDFI
                     ICSIHAIDTMDLYLGNYFKDKTQHEVYENYYLYLYNIVKNYKDYSVLGHLDLIKRYAP
                     YDTILDDRLFSDIIEETLKQAIYDGKGIEINTSCYRYNLPDLTPSKYILQMYKDLGGE
                     IITTGSDSHHISQVACEFDYIYSLLKNMGFKYVSKFNKLKPEFIKL"
     gene            9599..10921
                     /locus_tag="KRL67_001312"
     CDS             9599..10921
                     /locus_tag="KRL67_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HBH1522208.1"
                     /translation="MKVPYLVLSTVILFISNFIVRIFGFLYKIFLSRALGETGLGIYH
                     MIFNFLMICLAVTTTGIPTALSCLVAKRKALNDRHNTNALFISTLYISFFVALIISIV
                     ASFNSSFLSLKFLKDAKLNLFILAVCPAIVIITLSNVLRGYYYGIKNVKIPAIGQIIE
                     QIGKILFVFLLVMYINNKSMNCYIALLGISIGELSNIIFMLICLWRDSSFDNRYIISI
                     KDFYNSSMETLKMSIPITCNRMSNILLQSISSMMIPSRLALSGMTYQQSLSMYGIVSG
                     MVMPFIFLPFTVGSALIVNLIPTISQEMALRKRKSVIKKIKYSILLTLFVGILSSIFF
                     YFFGKDLCILVFKNTLAGEYLKAMFLVPLFMSLNQTLSGILHSIRKELASSINTITGM
                     LIQLIALYVFLPIPGLNIYAYIYTMTIVSIFTCLLHTIVLFKSLKSIH"
     gene            complement(11021..12238)
                     /gene="cdeC"
                     /locus_tag="KRL67_001313"
     CDS             complement(11021..12238)
                     /gene="cdeC"
                     /locus_tag="KRL67_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exosporium morphogenetic protein CdeC"
                     /protein_id="HBH1522209.1"
                     /translation="MQDYKKNKRRMMNQPMSTMNEEEVYTDEINSEDMRGFKKSHHHN
                     GCNTDNKCECHDDCNPCNPCNPCKPNPCNPCKPNPCDDNCGCHDNCKCDCEPCEMDSD
                     ECFENKCGPECCNPISPRNFSVSNAVPFAIEANRIFDTMQFQTFTDATGPNGEPLTFE
                     TEVVEVFGSVPSAGQASVTIEKICLSNDGIVIDTGMTTLEDFDLDPLGDIVGRNCETT
                     FEFAVCGERNAECCRQGKGKSVAYKQRGLTVAVRNLVLELRGRCGCTEFVALAFPAVR
                     AGGGCKRRVDYVEFTFNTLSAPICLPADGRAVTLRQEYQTNLTVDCIGKSILKLECNE
                     CCEPFYELIIPNDIDLVLCLQETVSTLISEQIVVLASPNPIQPRLVDTFSKVCDFSQC
                     GPNHGSGKPSCHR"
     gene            complement(12421..12601)
                     /gene="ssrS"
                     /locus_tag="KRL67_001314"
     ncRNA           complement(12421..12601)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="KRL67_001314"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(12716..13627)
                     /locus_tag="KRL67_001315"
     CDS             complement(12716..13627)
                     /locus_tag="KRL67_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH1522210.1"
                     /translation="MNNLLFLIGVIALSNGNISIFDDKNKSKGNNRVRKNKKNANDKL
                     SQSTHGNLRFDANDIKRGLRLMDLSEEDLEMGMEIITRTKKYMSRDERKILIKIESVL
                     DLVRGIKKLNNIDMVDIEDETDFFRKMDSDDKKNMMIKEIIDVFPEKRKNSVEKAIGM
                     KKKIDLFAELFLPDDFGEGGFSLSSLANINNLGSMNNLKLLGSLLRGDDSNNDDDEDN
                     NEELNYEDEELDCEGKELDYDDELTDEGEMEDNEDELVEDKNAGYENIENDEDIVYDE
                     ELYNINEQESEYNYNEKNTKTRAKKKR"
     gene            13839..14237
                     /locus_tag="KRL67_001316"
     CDS             13839..14237
                     /locus_tag="KRL67_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH1522211.1"
                     /translation="MKKLDTESLEKMAKQKNIDKDKIEKMADSYKGKSENELMEELIK
                     IGKNLDGRDEVVSKFKAFLDENQRKKLDNIMEKISDAENQRDTKPAKTKKAKPTKGKS
                     NSPAPKNTPGSQQKKSKSLFKKTKKSNPHE"
     gene            complement(14360..15376)
                     /gene="ccpA"
                     /locus_tag="KRL67_001317"
     CDS             complement(14360..15376)
                     /gene="ccpA"
                     /locus_tag="KRL67_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator CcpA"
                     /protein_id="HBH1522212.1"
                     /translation="MKGNITIKDVAKQAGVSISTVSRVINDSKPVTDEVKQKVLEVIK
                     ETGYIPNPLARSLVTKKSQLIGVIVPEVSDSFVNEVLNGIEEVAKMYDYDILLANTYS
                     DKEQELKSINLLRAKQVEGIVMISWIVEQEHINYIQNCGIPATYISKTARNYDIYTVS
                     TSNEEATFDMTEHLIKKGHEKIAFIMTSKDDTVLEMERLAGYEKALSNNNIELDKSLI
                     KYGGTDYESGYNSMKELLDDGIIPHAAFVTGDEAAIGAINAICDAGYKVPEDISVAGF
                     NDVKIARMYRPKLTTVYQPLYDMGAVAIRMVIKLINKELIENKKIELPYRIVDRESVT
                     ERKK"
     gene            complement(15629..15829)
                     /locus_tag="KRL67_001318"
     CDS             complement(15629..15829)
                     /locus_tag="KRL67_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH1522213.1"
                     /translation="MLDRFFGEEGCFGGGGWWIIVLFFLFLAFEDCWRDIDIMSWIPF
                     LILLLITCSCGGFFDGEVGCGC"
     gene            complement(15863..16042)
                     /locus_tag="KRL67_001319"
     CDS             complement(15863..16042)
                     /locus_tag="KRL67_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH1522214.1"
                     /translation="MLDRFFGEEGCFGGGGWWIIVLFFLFLAFGDSWAEFDIMAWIPF
                     LIVLLILCCCGGCLE"
     gene            complement(16224..16430)
                     /locus_tag="KRL67_001320"
     CDS             complement(16224..16430)
                     /locus_tag="KRL67_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH1522215.1"
                     /translation="MTKLEDNMERFFGGCEGFFGNKMLIIIVIVFLLLCTDILEDLLC
                     DDNIWIWVILIVLLLFNFDDGCCC"
     gene            complement(16478..16903)
                     /locus_tag="KRL67_001321"
     CDS             complement(16478..16903)
                     /locus_tag="KRL67_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH1522216.1"
                     /translation="MKYVGLLLTSVGMFLLIAVNFYYNSITLDMQRIEDYVMETNLIL
                     EDVAEKESYVSNEKEDYISRLMHVKKGIENSKTSFLIERYKEYKIKSIESLIYTISEE
                     KKDYLDEVDRYNKLGEKEINKLINKNFLEVTYLSITTYI"
     gene            17075..17302
                     /gene="acpP"
                     /locus_tag="KRL67_001322"
     CDS             17075..17302
                     /gene="acpP"
                     /locus_tag="KRL67_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HBH1522217.1"
                     /translation="MFEKIKEIIAEQLGVDNLDEITMEASLMDDLEADSLDAVEIIMA
                     LEDEFGIEIPDEEAENFKCIGDICKYIEENK"
     gene            complement(17446..18450)
                     /locus_tag="KRL67_001323"
     CDS             complement(17446..18450)
                     /locus_tag="KRL67_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein"
                     /protein_id="HBH1522218.1"
                     /translation="MIIHKFIIHVLDKNSDVPILNDFEGKVNQEVDGFFQKAIKRIAK
                     DEDLRKGVFKDYNDNLIKNCCEQIIYDESTFLKNSKEIASYLFDVMKINAALESCDLA
                     ICLYTIKDEKSVAILKLDYKKLYTHSIEFVDDKFNIQMVSNEIGIPETLRQKQGALIS
                     LSGINDEFHLRLLDKDAEKEGSESKFVTEFLNAKKIDDDKYKTKVFKNTAENWITNAL
                     SNDIKQAEDVRSILNYTLKEKHEVDINDFVDNSIKDDELKDSFKEHMEEKGLDESFSI
                     DKKWVEKKLKKRSIKTDNGFNIKGNLTDFEDPMKYTVKQNQDGTIDIIIKNVTFYEEK
                     "
     gene            complement(18464..19366)
                     /locus_tag="KRL67_001324"
     CDS             complement(18464..19366)
                     /locus_tag="KRL67_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HBH1522219.1"
                     /translation="MFKKENQRYNLISYTNEEEMTLKEVLLDKLNFSVRSLSKMKREK
                     SVLVNGVYKKPSLKVYSGDLIEVKIDEEKANFEPQDLNLQIIYDDFDIIMVNKPPFMV
                     VHPTKSHYDKTIANGISYYIDNQKENVKIRFVNRLDMNTSGLVIVAKNAYAHHTLSTA
                     MSENKVEKKYITVVDGIIKENEGTIDEPIYRPTEDSIKRIIDERGQSSITHYKVIERL
                     ENATVLEVSLETGRTHQIRVHMAHIGHGIIGDELYGCVDEKLINRQALHAYKLEFEQP
                     RTKEKLKFKADIPEDMKELISKLR"
     gene            complement(19562..20737)
                     /locus_tag="KRL67_001325"
     CDS             complement(19562..20737)
                     /locus_tag="KRL67_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000786547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HBH1522220.1"
                     /translation="MREVVIASAARTAVGSFGGAFKSVSAVELGVTAAKEAIKRANIT
                     PDMIDESLLGGVLTAGLGQNIARQIALGAGIPVEKPAMTINIVCGSGLRSVSMASQLI
                     ALGDADIMLVGGAENMSMSPYLVPSARYGARMGDAAFVDSMIKDGLSDIFNNYHMGIT
                     AENIAEQWNITREEQDELALASQNKAEKAQAEGKFDEEIVPVVIKGRKGDTIVDKDEY
                     IKPGTTMEKLAKLRPAFKKDGTVTAGNASGINDGAAMLVVMAKEKAEELGIEPLATIV
                     SYGTAGVDPKIMGYGPVPATKKALEAANMTIEDIDLVEANEAFAAQSVAVIRDLNIDM
                     DKVNVNGGAIAIGHPIGCSGARILTTLLYEMKRRDAKTGLATLCIGGGMGTTLIVKR"
     gene            complement(20825..21670)
                     /locus_tag="KRL67_001326"
     CDS             complement(20825..21670)
                     /locus_tag="KRL67_001326"
                     /EC_number="1.1.1.157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxybutyryl-CoA dehydrogenase"
                     /protein_id="HBH1522221.1"
                     /translation="MKLAVIGSGTMGSGIVQTFASCGHDVCLKSRTQGAIDKCLALLD
                     KNLTKLVTKGKMDEATKAEILSHVSSTTNYEDLKDMDLIIEASVEDMNIKKDVFKLLD
                     ELCKEDTILATNTSSLSITEIASSTKRADKVIGMHFFNPVPMMKLVEVISGQLTSKVT
                     FDTVFELSKSINKVPVDVSESPGFVVNRILIPMINEAVGIYADGVASKEEIDEAMKLG
                     ANHPMGPLALGDLIGLDVVLAIMNVLYTEFGDTKYRPHPLLAKMVRANQLGRKTKIGF
                     YDYNK"
     gene            complement(21768..22565)
                     /locus_tag="KRL67_001327"
     CDS             complement(21768..22565)
                     /locus_tag="KRL67_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HBH1522222.1"
                     /translation="MSTSDVKVYENVAVEVDGNICTVKMNRPKALNAINSKTLEELYE
                     VFVDINNDETIDVVILTGEGKAFVAGADIAYMKDLDAVAAKDFSILGAKAFGEIENSK
                     KVVIAAVNGFALGGGCELAMACDIRIASAKAKFGQPEVTLGITPGYGGTQRLTRLVGM
                     AKAKELIFTGQVIKADEAEKIGLVNRVVEPDVLMEEVEKLAKIIAKNAQLAVRYSKEA
                     IQLGAQTDINTGIDIESNLFGLCFSTKDQKEGMSAFVEKREANFIKG"
     gene            complement(22584..23594)
                     /locus_tag="KRL67_001328"
     CDS             complement(22584..23594)
                     /locus_tag="KRL67_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HBH1522223.1"
                     /translation="MGNVLVVIEQRENVIQTVSLELLGKATEIAKDYDTKVSALLLGS
                     KVEGLIDTLAHYGADEVIVVDDEALAVYTTEPYTKAAYEAIKAADPIVVLFGATSIGR
                     DLAPRVSARIHTGLTADCTGLAVAEDTKLLLMTRPAFGGNIMATIVCKDFRPQMSTVR
                     PGVMKKNEPDETKEAVINRFKVEFNDADKLVQVVQVIKEAKKQVKIEDAKILVSAGRG
                     MGGKENLDILYELAEIIGGEVSGSRATIDAGWLDKARQVGQTGKTVRPDLYIACGISG
                     AIQHIAGMEDAEFIVAINKNPEAPIFKYADVGIVGDVHKVLPELISQLSVAKEKGEVL
                     AN"
     gene            complement(23613..24395)
                     /locus_tag="KRL67_001329"
     CDS             complement(23613..24395)
                     /locus_tag="KRL67_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009524639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HBH1522224.1"
                     /translation="MNIVVCIKQVPDTTEVKLDPNTGTLIRDGVPSIINPDDKAGLEE
                     AIKLKEEMGAHVTVITMGPPQADMALKEALAMGADRGILLTDRAFAGADTWATSSALA
                     GALKNIDFDIIIAGRQAIDGDTAQVGPQIAEHLNLPSITYAEEIKTEGEYVLVKRQFE
                     DCCHDLKVKMPCLITTLKDMNTPRYMKVGRIYDAFENDVVETWTVKDIEVDPSNLGLK
                     GSPTSVFKSFTKSVKPAGTIYNEDAKTSAGIIIDKLKEKYII"
     gene            complement(24454..25590)
                     /locus_tag="KRL67_001330"
     CDS             complement(24454..25590)
                     /locus_tag="KRL67_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HBH1522225.1"
                     /translation="MDLNSKKYQMLKELYVSFAENEVKPLATELDEEERFPYETVEKM
                     AKAGMMGIPYPKEYGGEGGDTVGYIMAVEELSRVCGTTGVILSAHTSLGSWPIYQYGN
                     EEQKQKFLRPLASGEKLGAFGLTEPNAGTDASGQQTTAVLDGDEYILNGSKIFITNAI
                     AGDIYVVMAMTDKSKGNKGISAFIVEKGTPGFSFGVKEKKMGIRGSATSELIFEDCRI
                     PKENLLGKEGQGFKIAMSTLDGGRIGIAAQALGLAQGALDETVKYVKERVQFGRPLSK
                     FQNTQFQLADMEVKVQAARHLVYQAAINKDLGKPYGVEAAMAKLFAAETAMEVTTKAV
                     QLHGGYGYTRDYPVERMMRDAKITEIYEGTSEVQRMVISGKLLK"
     gene            complement(26299..26766)
                     /locus_tag="KRL67_001331"
     CDS             complement(26299..26766)
                     /locus_tag="KRL67_001331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaK/EbsC family protein"
                     /protein_id="HBH1522226.1"
                     /translation="MSISNVREYFKQFGKEDSILEFEQSSATVELAAEAAGVIPARIA
                     KTLSFKIGDDAILIVTAGDAKIDNKKYKAEFNCKAKMLTPEEVLEFTGHAIGGVCPFG
                     LKNSLKVYLDDSMKRFDSVFPACGSSNSAIELTCEEMEKFSKCEKWVDVCKNW"
     gene            complement(26845..27387)
                     /locus_tag="KRL67_001332"
     CDS             complement(26845..27387)
                     /locus_tag="KRL67_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH1522227.1"
                     /translation="MNKFIKVSFFILGIFTLFIAINKLVYRDMVSVKDYEINHLEEIV
                     FDFDSRRSRDIGTIVRFKISNNSKYSYKLNSAKMKFENCVENKKGINKCSAYVNIDLF
                     NEKNSITERTLKYGIKPNNQGYVDFVIPKGLNFDYRYFNESGMSVEYKGEYIVNIPMA
                     KGLYLVVGKNNDTWNANIME"
BASE COUNT         8655 a         4965 c         3026 g        10762 t
ORIGIN      
        1 agattaagaa gctaattgta atttttctgt aataaaaaac actttaaagt tgaattttga
       61 aaattcaatt tacgatattt ttattgtaag taccttacaa cattatacca aattattcct
      121 aaacaatcaa aaactccatg aatatttaaa ggttttctat tttaaaaatc acctttgact
      181 attaatttat caaatatgtt ataatagttt tagataattt aaggaggatt aaataatgga
      241 ttttaaacaa cttgaagtat ttgttgctgt tgcaaaacat cagagttttt caaaggccgc
      301 aagagaactt tttttaactc aacctactgt aagtgcacac atacagaatt tagagagaga
      361 gttggaaact gtccttataa atagaagcaa caaagttatt acactcacaa aatctggtga
      421 aattctttat gaacatgcta tatatatatt gaataattgc aaacgtgcaa tttatgatat
      481 aaaagaatat tcaggaaaaa tagaaggtat tatagatata gcatgtagtt ccatacctga
      541 aacttatatt ttacctgatt ttataaagag tttttctatg agttatccag atgtaaaatt
      601 ttccataagc cattatgatt ctcaatatgc tatatcagaa attttaaatg aacgaataag
      661 ctttggatta gttggttcta aaatcaataa tcctcaaata gagtacctag acttattaga
      721 tgatgaactt gttcttatca ctccttcaga ttttaaaatt gataataaaa ataattgtat
      781 cgatatagga gaattggctt atctaaattt tattatgaga aaagaagggt caggtacaag
      841 aaacttaata ctaaatacac ttagtaaaaa taactttcca gtaagtaaat taaatgtaat
      901 agctcatgta gaatcaaatg aggctattaa ggaaatggtt agattaggtc ttggagtatc
      961 ctttatatcg tacatttcag ctattgatta tctcaatgca ggtaaaataa aatgctataa
     1021 aataaaggat gttgatttta ctagaaaatt tttctttatt tactctaaaa agaaaacttt
     1081 ttccccatta gaagataaat ttctaaacag attatgtgag tatttcgaaa ttataatata
     1141 ataactttaa taaagtagta cttaatttta ggtttataac taattaataa gctaattata
     1201 actatcacat aaatgcttat aaattattat gaaccgtatt tcttttttat ttaatttagt
     1261 caaaaaatcc ctcgttcaaa tgaacttggg atttttttat ttacactact ccaatagcag
     1321 ctaatactat acaaataatc ataactgaaa ttaatatcgt agttgtcttt accttttttc
     1381 ctaataacca atacataata ccgaatatca tagctggaag catacatggc attatttggt
     1441 ctaagtaagt ttgaataggc tctgcccatt ctccactacc tacattaaca ctaagtttta
     1501 acattacatt agatgcagtc atacctccaa ttaccataag ccctaacatt gaagctgctt
     1561 ccataacttt tgaaactata cttgaaccac tcacatcttt aaagaattga gttccatact
     1621 taaatccttt atctagacat atatagcgta taataaaatg aggaacattt attattaaaa
     1681 ggaataaaat cggccccatt atgttaccct gagaagctaa agctatacca actccagctg
     1741 ctataagctt taatgttccc cagaaaaatg aatctcctat cccagaaact ggacccatta
     1801 aacttgattt aacagcattt atactattag catcaaattc tttattcttt gcattttctt
     1861 cttccatagc tccagatata cctaccaaaa gagtacttat atgaggagta actgacataa
     1921 attctaaatg tctttttaaa gccttagatt tcttttcttt atcatcttta tacaatctac
     1981 ggattattgg tgccatcatg tatgcatatg ctatattttg catacgttcg tagttccaag
     2041 atgaatctaa tgtcaatgac cttctaaaac atttccaaaa gtcttttttc gttattttac
     2101 tatctggtga aattgtctct aacttcttag aactcgttgt catctactat tacctccttc
     2161 tctcctataa agttaacata tattaaagct attgctaatc caaacaatgc aactcctgtt
     2221 actgttatgt ttaggtaagc aaccaatata aatcctaata ataaaaatgc tactgttttc
     2281 ttattaagca tcattctagc taacatggca aaacctattg ctggtattac gcctgctgca
     2341 acgtccattc catgagttac atatgatggt ataaatgaaa gtattttttc tattactgga
     2401 acacctaatg aaaatcctat acctactatt attgctaata ttaatttatt tcctatacca
     2461 aataataatt gcacacgttc tacgccttca atattcccct cttctgcata acggtcagca
     2521 atatgagctc cccatgtaag tactactgtc attaagaagt tatctataag caatactaga
     2581 gttgctatag gaactgcaag tacaactgct gctgatacat cttgacccgt taatattaca
     2641 aacgctgtgg caataataga accagacaca aaatcaggag gattacttgc cccaacagga
     2701 aatgcaccca taaatactaa ttctaatgtt gaacctatta ctatacctgt atgaagatta
     2761 cctagtgcta aaccaacaag tggtgcaaca actataggtc tattatgcat tgtagaacct
     2821 acaaaactat gcatatatgc aaagaatgca attagagtta tcaaaataac ttgtgtaaat
     2881 tccattttca tccccctaaa attatattaa tttcattaca tttgtagcat catcatcagg
     2941 aactaattgt acaaatacat ttacgccttc tgaatctaac tctttaatca tctttatatc
     3001 atcttttgat acatgcactg ccttagatat attttcacga ttatcatctg attttgttcc
     3061 accaagattt atagatttaa tagcttttac ctctttagca agtctgtaaa catcttctac
     3121 agattcacaa agtatcaata agttgtattt atcagtaaca cctgagttaa gtgcttttat
     3181 agaatctgct atgcttttca ttactaactt aacattagat ggtttagcca ttttaagtcc
     3241 tgccattctc aattcatctt ttaataagtc atcacttgct atcaatatac agtctgtgct
     3301 taaaaacttt gtccatgtaa atgctacttg tccatgtata agtctgtggt ctactcttac
     3361 taatttaatc attataattc ctccctaatt tttttgattt catccatgaa tgtttttacg
     3421 tgttctacac taacatcccc aaaatctttg taattatctt taaagtaact tcctattatt
     3481 gccatatctc ctaatttaag ctgttttttt gcattttcta atgtaactcc agcagctata
     3541 accaatggga agtctcctaa atttttacga aacagttcta ttttttccat tgatgtttct
     3601 tgacctgtag cattttctgt aactgctata gcatcacatc ttgtcattcc tatttttaaa
     3661 tcttcttcaa catcattttc tgataatact ggctgatatt taaaacgtac gcctccaatt
     3721 aaatacgctg ggcattttga acgttgtaat ttaaaaaatt cttctaaagt agcctcgtct
     3781 ctaggtttaa catgacctac tacagaatca acttgtatat aactagcatt atattttgtt
     3841 gccagttcaa atcccatagt atcaacattt aaacagttta ctccatatgg tattgacaaa
     3901 ttagattttg acacatattc aagtattctt tctaaatcat aataattacc atagtaattt
     3961 tctaacatta ttccatctac accattttct tcaaagattg taatctcttt tttagctctt
     4021 tcaaagatat cttctggagt atctcctttt aagtgtatca ttgctataat tggtttttta
     4081 cttttaaata ctgataatat tccctttttt ttcataaact aaaatacctc ttcttcttta
     4141 atttgtaatg tcacgctttg tattcctctt ctagattctt ccactaattg gtctatatct
     4201 tcaactgtta attcatcaga aaaccttgta agaagttcta ataccatatt caaattcatt
     4261 ccagaaaata acttaacatt atgtagatta gttaatctta acattgctgt acatacactt
     4321 gctccatata agtcggataa aattatatat tcacattctt tatcttctga tattgttttt
     4381 tctaattcta aagcaaaatc tgaagcactt tgtccagggt acaaaccata cgaactaaca
     4441 caatctgcta aatctcctac tatcattttt actgcattta aaagcccttt tgaaaagtct
     4501 ccatgagatg ctaatattat ctttttcata ctcatcctcc tactaaatta tagattatat
     4561 ttctgctgat actgcatatt tgtacttatc acttcttata aattgctcat tgtaatataa
     4621 gacatcatta ttttctgtat acaccgattg tcttactttc aatagtggca tattacttgc
     4681 acatttaaga tggtcagaat attctttgtt tgaatgtttt acttcaaata tattttctac
     4741 tcgtttaggt aatgtgctta tattattaga tataacctcc attaaagatt tatcttctag
     4801 ttcttctttt aataaaaaca tgtaatctat tctaaaataa tcaacttcaa aaataacagg
     4861 tacttcatct attaatctaa gtcttttaat acatattacc ttctcttcta gttcaatact
     4921 taaagcttca gctacctgct tatctgcttt ttttattaaa acagagatta tttttgtact
     4981 tggttttgca ttaatacgat gacaagaatt agtaaaacta tttcctgcac atgttgcttc
     5041 tattctctct ggtattgtta cataagtccc tttaccacgt tttttacata atatatcttc
     5101 ttttacaagt tcattaagtg catttctcac agttatacga cttatattaa attctttaca
     5161 caagtctcct tcagatggaa gtttatctcc tggtttatat atgccagatg ctatttgttt
     5221 ttttatgttg tcatatagtt gttgatataa agggacaaag gaatttactt ctaagtccat
     5281 ttcttttttt tgtctctttt catccatttt gctcacctcc ctatgtaaca tttttataac
     5341 attatataac aagttcttta ttacatatta taacaagatg aaatcgttgt caattttttt
     5401 ttaaataatt tttaatttga cagtattttc ttttattttc tctagtgtga caaattgcta
     5461 tttttacata aaaataagac tgttgtacaa caaattaaat tgtttatacg acagccttta
     5521 ttcacttatt acacttatat ttttacttat aaatttatat taaaaaatgc cttatatccc
     5581 ttatacattt catatatgtc tactttagag acaatctcat gtggtgcatg catatttaaa
     5641 actccaacac cacaatctat aacctctgca ccttgatttg ccaatatatg ggctatagtt
     5701 ccgcctcctc cttggtctac ttttccaagt tcagcagttt gccatactac atttccttta
     5761 ttaaaaattc tctttacttc tgacataaat tcagcattag catcattaca tccactcttt
     5821 cctcttgaac ctgtatactt agttaaaact actccatttc ccatatatgc tgaatttctc
     5881 ttttcatatg cttctccaaa atttgggtca tatccagcag aaacatcagc tgataatacc
     5941 ttactatttg ctaaagctct tcttacttta atatcacaat aatctccttc taaagcaatc
     6001 agttcagcca cagtattttc aaaaaacttt gagtgcattc ctgtattccc tttagaacca
     6061 atttcttctt tatctacaca taatgcaact gaagtaaatt ctggttgttc agtttctagt
     6121 atagctttta cagcagcata tgaacatacc ctatcatcat gcccatatcc caacaccata
     6181 gacctatcaa gccctaaatc tctagcctta ccagctggaa ccacttcaat ttcagcactt
     6241 aataaatctt cttcaactat attgtatttc tcatttaaaa tctttaatat atttgctgtt
     6301 ataggttctt tttcttcatc ctctaaagga atattcccaa ttaatatatt taaagcttct
     6361 ccactaattc cttctgatag tttcttttgc atttggtctc cagctaaatg aatcaataaa
     6421 tcagttacac aaaatactgg gtcactatct tcttccccaa tagatatgtc cacttttgtt
     6481 ccatcattta atataactat gccatgtaaa gcaagtggaa cagccgtcca ttggtatttt
     6541 tttattccac cataataatg agtcttaaaa aaacctagat tactctcttg atacagtgga
     6601 tttggtttta aatctaatct tggcgaatct atatggcttc ctataatctt catacctttt
     6661 tctatataat ttttacctat tacaaaaagt gctactgcct tctctttatt tataacataa
     6721 atcttatcac cacaacttat gcttcctttt tccatagctt catctattga aatatagtta
     6781 ttttctttag ccaattttat aatttctttt actgaaagtc tttcagtctt tgacttactt
     6841 aaaaactcca tataattgcg agaatattgc ataatttgtt caatagtatt ttcttttctt
     6901 tcaaaatccc aagcattctt aaattcgtac tttaagttca tattttcctc ctaataagtt
     6961 ctaattttta ttgtttttca tgtattgctc atattcttct ggtgaaacta ctttgacact
     7021 atctagtctt gatctaaagt gggctctaaa gttctctaaa tattctttcc ttaaaatttc
     7081 tctttctttg atttcatcag cagaaaggat tccctcttta tttttctttg ctaattcatt
     7141 aatcctatct aatttcttct tgtcaaacat tttttcctcc taaaaaactt ctatttattt
     7201 aataaaataa tttattgctt tttatatcta acttgttttt atattcacaa aaaaactcat
     7261 ctatattatc acttattata gatattccct tttctagttc ttccatgctt gggcgagcta
     7321 tatttatcct aaagaatcta tcatccacta gattgtcaaa aaaataagtt cctggaagca
     7381 ttgacactcc tttgtttaac ataaaatttg taaatacttg agatgtatat cctcttggta
     7441 gttctaaaaa aaaatttatt cctccacaag attttctaac ttttaattta ccactcaatt
     7501 tgtcattaat caagtttttt gccaatttat acttatcagt atatatcttc tctatatgac
     7561 tcaaataatt cttccaatca aaattattca tatagtaata cattgatttt tgaattaaac
     7621 ctggtgttga tatatctgat gaatatttag cccataaaac tttttttaac aactcatttg
     7681 gcatttctac aattcctatc ctcagacctg gcataagtat ttttgaaaag ctttttatat
     7741 atactactct atttttatca tcataacttc taattgttct attgtccttt gattcaaaaa
     7801 caaaatcact tataaaatca tcttctatta tataaaaatc atgttcttct gccaattcta
     7861 tcaatttctt ttttttatat gttgagtaag atattccagt tggattttga aaattaggca
     7921 taatgtatat aagtcttggt tttatttttt ccaactttaa ttttaaaata cctatatcaa
     7981 ttccatcatc taacataggt atagatatta tttttgcacc tctactttta aacacttcaa
     8041 ttgctccatt gtaagaaggt tcttccataa aaacaacatc tgaataactt ataagtcctt
     8101 tacaaactat atctatacct tgttgagcac ctgatattat ttgaatattt tccttagtag
     8161 tttttatatt tccatcactt agatactcta ccattttttc tcttaattct tcagagccaa
     8221 gaccttcatc atattcaaat attgaactac catctttaga taatgccata tttacagcat
     8281 ttttaaatga atcgatagga aacatatcat tagatggatt cccattgtca aaatgaatta
     8341 aatcttctct ttgcacaata atcttatttt tcctgttatc tataccactt ttataagaat
     8401 ttaaacttga tacaaaagta ccactcccta taattttata tacatatcct tcattttcta
     8461 aaagctcgta taccttaact atagtagtgt tatttacttg taaaaaattt gatagttttc
     8521 ttattggagg caatttttca tgttctacta actctttatt tattatcaat tcttttatat
     8581 gattaaatat ctgtatatat ttagttgatt ttttctcatt taaatctatt ctgtattttt
     8641 ccaaatttat caccgctcat tgtatttata ttttattgat gcatttatgt atatttgata
     8701 ttatttagta tgtatatata ataccaaaat ttttataaaa aggggaaata tttatgtttt
     8761 acgactatca catgcactca agtttctcta cagacggaaa atctacaatg gaagaaatgg
     8821 taaaaaaatc tattgaactc ggtttggagg aaatttgttt cacagaccat gttgactatg
     8881 atgtatatgc tgatgattct ttttcaattg tctacgaaga ttattttaag agtttagaga
     8941 ctcttcaaag caaatataaa gataaaatat caataaaaaa aggaattgaa tttggagttc
     9001 aaactcaatt aatggataca tacaaaaagg aagcacatca atatccattg gactttataa
     9061 tatgttctat ccatgctata gatacaatgg acttatacct tggtaattat tttaaagata
     9121 aaactcaaca tgaagtttat gaaaattact acctttattt atataatatt gttaagaatt
     9181 ataaagatta ctctgtctta ggtcatttag atttgattaa aaggtatgca ccttatgaca
     9241 ctattttaga tgatagacta ttttctgata taattgaaga aactcttaaa caagctattt
     9301 atgatggcaa aggtatcgag ataaatacct cttgttatag atataatctt ccagacctaa
     9361 ccccatctaa gtatatactt caaatgtaca aagacttggg aggggaaatc ataaccacag
     9421 gttcagattc acatcatata agtcaagttg catgtgagtt tgactacata tattcattac
     9481 taaaaaatat gggatttaaa tatgtatcta aatttaataa attgaaacca gaatttataa
     9541 aattataaat ttttacacct ccttgtgaat atatttaaaa gataaggagg tgattttttt
     9601 gaaagtacct tatttagtac tatcaacagt tattttattt atatccaatt ttattgtaag
     9661 aatatttggt tttttatata aaatattctt atcaagagca ctaggtgaaa caggtttagg
     9721 tatctatcat atgattttta actttttgat gatttgctta gctgttacaa caacaggtat
     9781 acctactgca cttagttgtc tagttgcaaa gagaaaagct ttaaacgata gacacaatac
     9841 taatgcttta tttatatcaa ctttatatat atcatttttc gtagcactaa ttatatctat
     9901 agttgcatct ttcaatagtt cttttttatc acttaaattt ttaaaagatg ctaaattaaa
     9961 tctatttata ctagctgttt gtcctgctat agttataata actctttcaa acgtactaag
    10021 aggatattac tatggcataa agaatgtaaa aatccctgct attggacaaa tcattgaaca
    10081 aattggtaaa atattatttg tttttttact tgttatgtac ataaataata aatctatgaa
    10141 ttgttatata gctctattag gtatatcgat tggtgagttg agtaatatta tatttatgct
    10201 tatttgctta tggcgagatt catcatttga caataggtat attataagca taaaagattt
    10261 ttataattct tctatggaaa cattaaaaat gtctattccc ataacttgta atagaatgtc
    10321 aaacatactt ttgcaatcta taagttctat gatgatacca tcaagattag cattatcagg
    10381 aatgacttat caacaatctt taagtatgta tggtattgtc agtgggatgg taatgccttt
    10441 tatattttta ccttttactg ttggttcagc gcttattgta aatttaatac caactatttc
    10501 acaggaaatg gctctaagaa agcgcaaaag tgtaattaaa aaaatcaaat actccatact
    10561 tcttacttta tttgtaggca tactatcatc aatatttttt tatttctttg gaaaagattt
    10621 atgtatatta gtatttaaaa atacacttgc aggagaatat ctaaaagcta tgtttttagt
    10681 tccactattt atgtccctaa atcaaactct atctggcatt ttacattcaa taagaaaaga
    10741 attagcttca agtataaata ctattactgg tatgttaatt caactaatag ctttatatgt
    10801 atttcttcca attccaggac ttaatattta tgcatatata tacacaatga cgatagtatc
    10861 aatctttact tgtctattac atactatagt gctttttaaa tcactaaaat ctatacacta
    10921 gttataaaaa cgtgaaggct accaatgtaa attatacact ttggtagcct tccttagatg
    10981 atttttattt ataatttaaa ctgtctatat atctacctat ctatctgtgg caacttggct
    11041 ttccacttcc atgattaggt ccacattgcg aaaaatcaca tactttagag aaagtatcaa
    11101 caagtcttgg ttgaattgga tttggtgatg ctaaaactac tatttgttca cttattaatg
    11161 tgctaactgt ttcttgtaag caaagtacta aatctatatc atttggtata attaattcat
    11221 agaaaggttc acaacattcg ttgcattcta attttaatat agattttcct atacaatcta
    11281 cagttaagtt agtttgatat tcttgtctta aagtaacagc tcttccgtca gctggcaagc
    11341 atattggtgc tgaaagtgtg ttaaaagtaa attctacata atcaactctt ctcttacatc
    11401 cacctcctgc tctaactgct gggaaagcta atgcaacgaa ctctgtacat ccacatctac
    11461 ctcttagctc tagtactaaa ttacgaactg ctacagttaa tcctctttgt ttataagcta
    11521 ctgatttgcc ttttccttgt ctacagcact cagcgtttct ttctccacaa actgcaaatt
    11581 caaaagttgt ttcacagttt cttcctacta tatctcctaa tgggtctaaa tcgaaatctt
    11641 ctaaagttgt cattcctgtg tctataacta ttccatcatt acttaagcat attttttcta
    11701 tagttacact tgcttgacct gcacttggaa ctgaaccaaa tacttctact acttctgttt
    11761 caaaagttaa tggctctcca tttggtcctg ttgcatctgt aaatgtttgg aattgcatag
    11821 tatcaaatat tctattagcc tctattgcaa atggcactgc atttgataca gagaagtttc
    11881 ttggagatat aggattacag cattctggtc cacatttgtt ttcaaaacat tcatctgaat
    11941 ccatttcaca tggttcacaa tcacatttac aattgtcatg gcatccacaa ttgtcatcac
    12001 atggattagg tttgcatgga ttgcatgggt taggtttaca tggattacat gggttgcatg
    12061 gattgcaatc atcatggcac tcacacttat tatcagtatt acatccatta tgatggtgtg
    12121 attttttaaa acctctcatg tcttctgaat ttatttcatc tgtatacact tcttcttcat
    12181 tcattgtaga cattggctga ttcatcattc ttcttttatt ttttttataa tcttgcattt
    12241 ttcattgccc tccctgtaag cgattcttat taagtctttg gctttttgaa gcctaattaa
    12301 tactatgctt ttataatctt aattgttact tgctttttga atttatcaat aattaggcgt
    12361 atttagtaga aaaatgcata ttaataaaaa aagtactact atttgaaata gtagcacttt
    12421 ttttgtcccg aaacaccggt ggctaagtaa attcacaccc taggcactcc caggtgggtg
    12481 acttgccagt aacctttaac gtccaaacta actaggcatg ttataattta ccagacacgg
    12541 tctcccgtat ttcatttgta ggttgaattt aaaaaagcct tatcgtatgt ttcaggattt
    12601 catattatta ttatataata aattttgaat tttttaaagt ggttttatat agtaattgag
    12661 ataatcttat tctatcatta ctttttcttc cttttttctt tatgtattta gttaattatc
    12721 gttttttctt agctctagtt ttagtatttt tttcattata attgtattcg gattcttgct
    12781 cattaatgtt gtatagttcc tcatcgtata ctatatcttc atcattttct atattttcat
    12841 agcctgcgtt cttatcttct actaattcat cttcattatc ctccatttca ccttcatctg
    12901 ttaactcatc atcataatcc aattctttgc cttcacaatc taattcttcg tcttcataat
    12961 ttaactcttc attattgtct tcatcatcat cattattact atcatcacct cttaaaagac
    13021 ttcctaacaa tttgagatta ttcatactgc caagattgtt tatattggcc aaagaactca
    13081 aactaaatcc accttcacca aagtcatctg gtaaaaataa ttctgcaaat aaatctatct
    13141 tctttttcat tcctatagct ttttcaactg aattttttct tttttctggg aatacatcta
    13201 taatttcctt tatcatcata ttctttttat catcactatc catttttctg aaaaaatccg
    13261 tttcatcttc tatatctacc atatctatat tatttaattt ttttattcct ctaactaaat
    13321 ctaaaacact ttctattttt attagtattt ttctttcatc tcttgacatg tattttttag
    13381 ttcttgttat tatttccatt cccatttcca aatcttcttc acttaaatcc atgagcctta
    13441 aacctctttt tatgtcattt gcatcaaacc ttaaattgcc atgtgtagac tgtgaaagtt
    13501 tatcatttgc attttttttg ttttttctaa ctctattatt tccttttgat ttatttttat
    13561 catcaaatat agagatatta ccattactca atgcaatcac tccaatcaaa aatagtaagt
    13621 tattcaagac aattccccct tatattatat aattgcccta tattaaagta tgtttttcat
    13681 attttaatgt tccattttta aagctcagtg catatatatt atctataaga atttttattt
    13741 atattatata tcactcacaa ttcaccactt caatattatt tctatatata aatagtatga
    13801 atataattca atatatattt atagaaaggg gtttttttat gaaaaaatta gatacagagt
    13861 cattagaaaa aatggctaaa caaaaaaata ttgacaagga taaaatagaa aagatggctg
    13921 atagttacaa aggaaaatca gaaaatgaac ttatggagga acttataaaa ataggtaaga
    13981 acttagatgg aagagatgaa gtagtatcaa aattcaaagc atttttagat gaaaatcaac
    14041 gcaaaaaact agataatata atggaaaaaa tctcagatgc agaaaatcaa agagatacta
    14101 aaccagctaa aactaagaag gctaaaccta ctaaaggtaa gtcaaattca cctgcaccaa
    14161 aaaatacacc aggtagtcaa caaaaaaaat ctaaaagttt atttaagaaa actaaaaagt
    14221 ctaaccctca tgagtagata aaaataaact atttaagtaa ttagataatt atttttagtt
    14281 aatctattat taaatttgat attattgaca taaaaaataa ggtagtgcct taggcactac
    14341 cttattgatt ttaatagttt tatttttttc tctctgtaac actttctcta tcaacaattc
    14401 tataaggtaa ttctattttc ttattttcaa ttaattcctt atttattaat tttataacca
    14461 ttcttattgc tactgctccc atatcgtata gaggttgata tactgtagta agtttaggtc
    14521 tatacattct agctatctta acatcattaa atcctgcaac tgatatatct tctggaacct
    14581 tatatccagc atcacatata gcatttatag cacctatggc agcctcatca cctgttacaa
    14641 aagccgcatg aggtattatt ccatcatcta atagttcttt catactatta tatccactct
    14701 cataatcagt tccaccatac ttaatcaaac tcttgtctaa ctctatattg ttatttgaaa
    14761 gtgctttctc ataaccagca agtctttcca tttctaaaac agtatcatct ttactagtca
    14821 ttataaaagc tatcttttca tgacctttct tgataagatg ctctgtcata tcaaaagtag
    14881 cttcttcgtt gctagtactt actgtgtata tatcgtaatt tctagcagtt ttacttatat
    14941 atgtcgctgg tattccacaa ttttgtatat agttgatatg ttcttgttca actatccacg
    15001 aaatcataac tataccttcc acttgttttg ctctcaatag atttatactc ttaagttctt
    15061 gttccttatc agagtatgta ttcgctaaaa gaatatcata gtcatacatt ttagcaacct
    15121 cttctatccc atttaacacc tcattaacaa aagaatctga aacttctgga actattaccc
    15181 ctattaattg actcttcttt gttactaagc ttctagcaag tggatttggt atatatccag
    15241 tctcttttat aacctctaaa actttttgtt tgacttcatc agttacaggt tttgaatcat
    15301 ttataactct agatacagta gatattgaca ctcctgcttg tttagcaaca tcttttatcg
    15361 ttatattgcc tttcatcttc atcctcctta ttttactaaa tttatctaaa actgaaccaa
    15421 atttttgaag gttcctattt taactacatt tatataagtt tttattttac cataaaagca
    15481 agtacattta aatacattat attttatatt ataccctata ttcaacaaat ttaataatat
    15541 tcattcttaa tttaaatcat attaaaaaat agaataaaaa aatgctggta taaaatacca
    15601 gcattttttt attctaatga tttaagtatt agcagccgca acctacttca ccatcaaaga
    15661 atccaccaca tgaacaagtt attagcaata atattaagaa aggtatccat gacattatgt
    15721 caatgtctct ccagcaatct tcaaaagcta agaataggaa gaatagtact attatccacc
    15781 atcctcctcc accaaagcat ccttcttctc caaaaaatct atctaacata acaaagccct
    15841 ccttttttta ataactgtgt tcttattcta agcatccacc acagcagcaa agtattaata
    15901 aaactattag gaatggtatc catgccatta tgtcaaattc tgcccagcta tctccaaaag
    15961 ctaagaatag gaagaatagt actattatcc accatcctcc tccaccaaaa catccttctt
    16021 ctccaaagaa tctatctaac attatacgtt acctcctgtt gatattttgt tgagcttttc
    16081 gctcctaaca tatactatgc atttggtttc cattttgcta atgtattttt actatttttt
    16141 taaatttttt aatttgaact aaaaaaggta aaaccataac ttgtataagt tactggtttt
    16201 acctatttaa taatcaatat tttttagcaa caacaaccat catcgaagtt aaataataac
    16261 aatactatta atataaccca tatccatata ttgtcatcgc ataataagtc ttctaaaatg
    16321 tctgtgcata atagtaagaa tactataact attattatca acattttatt gccaaaaaat
    16381 ccttcgcatc ctccaaagaa acgttccata ttgtcttcta attttgtcat aactaatcct
    16441 ccttgaatca ttaagagact aagttttgtc tctttaatta aatatatgta gtaatagaca
    16501 gataagttac ttctaaaaag tttttattga ttagtttatt tatttctttt tcaccaagtt
    16561 tattgtatct atcaacttca tccagataat cttttttctc ctctgatata gtatagatta
    16621 aactttctat tgattttatt ttgtactcct tatatctttc tatcaagaag gatgtttttg
    16681 aattttctat tcctttcttt acatgcatta accttgatat gtagtcttct ttttcattag
    16741 atacataact ctccttttca gctacatctt caaggattag attggtctcc attacataat
    16801 cctctatacg ttgcatatct aaagttattg aattataata aaaatttact gctataagta
    16861 aaaacattcc tactgaagtt agtaatagtc ctacatattt catagcattt cctcctaagt
    16921 tatatttgtt atcaataaag tttattcatc aaataatact ttattactac atttataaaa
    16981 ttttattatt ttttatagat tccatattga aaaattcttg atattatatt aaactaacat
    17041 taaaattaat taaaactgct ttaggaggct taaaatgttt gagaaaataa aagaaataat
    17101 agcagaacaa ttaggggtag acaatttaga tgagataact atggaagcat ctttaatgga
    17161 cgacttagaa gctgattcat tagatgctgt tgaaattata atggcccttg aagatgaatt
    17221 tggaatagaa ataccagacg aggaagctga gaactttaaa tgtattggtg atatatgtaa
    17281 atatatcgaa gaaaacaaat agtatcgttt tgtctacaaa tataaatagt ctgtgtaaac
    17341 ttctacataa taaattatat tagagttcgc acagactaga ttacatttat ttacaatata
    17401 taaaatacac gctcttatat ttaatttata tactaagtat ataaattact tttcttcata
    17461 aaacgtaaca ttctttatta ttatatctat agttccatct tgattttgtt ttacagtata
    17521 cttcattggg tcttcaaagt cagttagatt cccttttatg ttaaaaccat tatccgtttt
    17581 tatacttctt tttttaagct ttttctcaac ccatttttta tctatactaa aactttcatc
    17641 aagacctttt tcttccatat gctctttaaa gctatctttt aactcatcat ctttaattga
    17701 attatcaaca aagtcattta tatcaacttc atgtttttcc tttagtgtat aatttaatat
    17761 acttcttaca tcttcagctt gctttatatc attactaaga gcattggtta tccaattttc
    17821 agctgtattt ttaaatacct tggtcttgta cttatcatca tctatttttt tagcatttaa
    17881 aaattctgtt acaaacttag attctgaccc ttctttttca gcatccttat ctaaaagcct
    17941 tagatgaaac tcatcattta taccacttaa actaatcaaa gctccttgct tttgtcttaa
    18001 agtctctggg ataccaattt catttgaaac catttgaata ttaaatttat catccacaaa
    18061 ttcaattgaa tgagtgtaca actttttata atctaatttc agtatggcta cactcttttc
    18121 atctttaata gtgtataagc aaattgccaa atcacaagat tctagtgcag cattaatttt
    18181 cataacatca aacaagtaag atgcaatttc tttagaattt ttcaaaaaag tactttcatc
    18241 atatattatt tgttcacaac agtttttaat taaattatca ttataatctt taaaaacacc
    18301 ttttcttaaa tcctcatctt ttgcaattct ttttatagct ttctggaaaa aaccatctac
    18361 ttcttgatta actttgcctt caaagtcatt taatattgga acatcactat ttttatccaa
    18421 aacatgtatt ataaatttat gtattatcat atatatcacc ccattatctt aatttcgata
    18481 ttaactcttt catatcctct ggtatatctg ctttaaattt caatttttct tttgttcttg
    18541 gttgttcaaa ctctagcttg tatgcatgaa gtgcttgcct atttatgagc ttttcatcta
    18601 cacaaccata caactcatca cctattattc catgacctat atgagccata tgtactctta
    18661 tttgatgtgt tctaccagtt tctaagctta cttctaatac agttgcattt tctagtcttt
    18721 ctattacttt atagtgagta atagatgact gtcctctttc atctattatc ctctttattg
    18781 aatcttctgt aggtctataa attggctcat ctatagttcc ctcattttct tttataattc
    18841 catcaactac tgttatatac tttttttcaa ctttattttc actcattgct gtagacaaag
    18901 tatgatgtgc ataagcattt tttgctacta taactaaccc tgaagtattc atatctaacc
    18961 tatttacaaa tcttattttt acgttttcct tttgattatc tatatagtaa ctaataccat
    19021 ttgctattgt tttatcatag tggctttttg ttgggtgaac taccataaat ggaggtttat
    19081 tcaccatgat tatatcaaaa tcatcataaa ttatctgtaa atttaaatct tgaggttcaa
    19141 aatttgcttt ttcctcgtct atttttactt ctattaaatc accactatac acctttaaac
    19201 tcggtttttt atatactcca tttactaaaa cgctcttttc cctcttcatt tttgataatg
    19261 acctaactga gaaatttaac ttgtccaata aaacttcttt taaagtcatt tcttcttcat
    19321 ttgtatacga tataagatta tatctttgat tctctttttt aaacaaactt ttacctactt
    19381 tcttttatat cttcctactt ttttatatct tcaaaatcat gatattatat attttacact
    19441 atttttgttt aatagataat aattattttt aactataaaa acctgtaaga ataacaaata
    19501 gttattctta caggttaaat tcatagttaa atatttaatt tgtaaatatt ataatctaaa
    19561 actatctctt aactattaaa gtagttccca ttccaccacc tatacaaagt gtagcaagac
    19621 cagttttagc atctcttctc ttcatttcat ataaaagtgt agtaagtatt cttgctcctg
    19681 agcatcctat tggatgtcct atagctattg ctccaccatt gacattaact ttatccatat
    19741 ctatatttaa gtctcttatt acagctacag attgagcagc aaatgcctca ttagcttcaa
    19801 ctaaatctat atcttcaata gtcatattag cagcttctaa agctttttta gttgctggaa
    19861 ctggtccata tcccattatt ttagggtcaa caccagctgt tccataagaa actatagttg
    19921 caagaggctc tattcctagt tcttcagctt tttctttagc cattactact agcatagcag
    19981 caccatcatt tattcctgat gcattaccag cagtaactgt tccatctttt ttaaatgcag
    20041 gtcttaactt agcaagtttc tccattgtag tgccaggctt aatatattca tctttatcta
    20101 ctatagtgtc accttttctt ccttttataa caacaggaac tatttcttca tcaaattttc
    20161 cttcagcttg agctttttca gctttatttt gacttgcaag agctaattca tcttgttctt
    20221 ctctagttat attccattgc tctgctatgt tttcagcagt aatacccatg tgatagttat
    20281 taaatatgtc tgataatcca tcttttatca ttgaatcaac aaaagcagca tcacccattc
    20341 ttgcaccata tctcgcactt ggtactaaat aaggagacat actcatgttt tcagctccac
    20401 caactaacat tatatcagca tcacctaatg ctataagttg agatgccatt gaaacagatc
    20461 ttaatccaga accacaaact atatttatag tcatagctgg tttttctact ggtatccctg
    20521 ctcctaatgc tatttgtctt gctatatttt gtccaagacc tgctgtaagt actcccccta
    20581 aaagagattc atctatcata tctggagtta tattagctct ttttatagct tctttagctg
    20641 ctgttacccc taactctacc gctgaaactg atttaaatgc tcctccaaaa cttcctactg
    20701 ctgttctagc tgcactggca attactactt ctctcatatt cattaccccc attaaattag
    20761 attttccata tatccgaaat tgttttttta aacaatttta tttaaatttt taattcccaa
    20821 actcttattt attataatca tagaatccta tcttagtttt tcttcctaat tgattagctc
    20881 taaccatttt agctaaaagt ggatgaggtc tatatttagt atctccaaac tcagtatata
    20941 aaacattcat tatagctaaa acaacatcta atccgattaa atcacctaat gctagtggtc
    21001 ccattggatg gtttgctcct aatttcatag cttcatctat ttcttcttta cttgcaacac
    21061 cgtctgcata tataccaaca gcttcattta tcataggtat aagtattcta tttactacaa
    21121 atccaggaga ttcagataca tctactggta ctttattgat actcttagat aattcaaata
    21181 ctgtatcaaa agtaactttt gatgttagct gaccacttat aacttcaact aatttcatca
    21241 taggaactgg attgaaaaaa tgcattccta taactttatc tgctctctta gtagaagaag
    21301 ctatttctgt tatagataat gatgaagtat ttgttgccaa gatagtatct tctttacata
    21361 attcatctag taacttgaaa acatctttct ttatattcat gtcttctaca gatgcttcta
    21421 ttattaaatc catatctttt aaatcttcat agttagtagt tgaactaaca tgacttaata
    21481 tttctgcttt tgtagcttca tccatttttc ccttagtaac taacttagtt aaatttttat
    21541 ctaataaagc taaacattta tctatagcac cttgagttct actctttaaa catacatcat
    21601 gtccacaact tgcaaaagtt tgtacaatac cacttcccat agttccacta cctattacag
    21661 ctaatttcat agttataacc tcctgttatt ttagtattta tattctgtta actagattaa
    21721 aatactattt aatctagtta acagcaaata tatttttaat tagattttta cccttttata
    21781 aagttagctt ctcttttttc aacgaaagct gacattcctt ctttttggtc tttagttgaa
    21841 aaacaaagac caaataaatt agattctata tctattccag tatttatatc agtttgagca
    21901 ccaagttgta ttgcttcttt agagtatcta actgcaagct gagcattttt ggctattatc
    21961 ttagctagtt tctcaacttc ttccattaaa acgtctggct caacgactct atttactagc
    22021 cctatttttt cagcttcatc agcttttata acttgacctg taaagattaa ttcttttgct
    22081 tttgccattc caaccaatct tgtaagtctt tgagttcctc catatcctgg agttattcca
    22141 agagttactt ctggttgacc aaatttagct ttagcagatg caattcttat atcacatgcc
    22201 attgcaagtt cacatcctcc acctaaagca aatccattta cagcagctat cactactttt
    22261 ttactatttt ctatttctcc aaaagctttt gctcctaaga tactaaaatc tttagcagct
    22321 acagcatcta aatctttcat gtatgcaata tctgctccag ctacaaatgc ctttccttcc
    22381 cctgtcaata ttacaacatc aatagtttca tcattattaa tatctacaaa tacttcataa
    22441 agttcttcta aagtctttga atttattgca ttaagggctt taggtctatt cattttcact
    22501 gtacatatat ttccatctac ttcaacagct acattctcat aaactttaac atcacttgta
    22561 ctcataatac cctccccaag aaattagtta gctaaaactt cacctttttc ttttgcaaca
    22621 cttaactgac tgataagttc tggaagcact ttatgaacat ctccaactat accaacatca
    22681 gcgtatttaa atattggagc ttctggattt ttatttatag caactataaa ctcagcatct
    22741 tccataccag ctatatgttg tattgctcca gatataccac atgctatata aaggtctggt
    22801 cttacagttt taccagtttg accaacttgt cttgctttat ctaaccaacc tgcatctata
    22861 gtcgcacgag aaccagaaac ttctccacct ataatttcag ctaattcata aagtatgtct
    22921 aagttttctt ttccacccat tccacgtcca gcagaaacta atatcttagc atcttctatt
    22981 ttaacttgtt ttttagcttc ttttattact tgtacaactt gaactaattt atcagcatca
    23041 ttaaattcta ccttgaaacg attaattaca gcttctttag tttcatcagg ttcatttttc
    23101 ttcataaccc ctggtctaac tgtagacatt tgaggtctga aatctttaca aactattgtt
    23161 gccattatat ttccaccaaa ggcaggtctt gtcattaata ataattttgt atcttcagct
    23221 actgcaagac ctgtacagtc agcagtaaga cctgtatgta ttctagcaga aactctaggc
    23281 gctaaatctc tacctattga agttgcacca aataatacaa ctatagggtc agctgctttt
    23341 attgcttcat aagctgcttt tgtatatggt tcagttgtat acactgctaa agcttcatca
    23401 tctactacta ttacctcatc tgcaccatag tgtgctaatg tatctattaa accttctacc
    23461 ttactaccta aaagcaatgc agaaactttt gtatcataat cttttgctat ttctgtagcc
    23521 tttcctagta attctaaaga aacagtttga attacatttt ctctttgttc tattactact
    23581 aaaacgttac ccattcttta tcccccctta aattatatga tatacttctc ttttaattta
    23641 tctatgataa ttccagctga tgttttcgca tcttcattgt atattgtacc agctggttta
    23701 actgattttg taaatgattt aaatacacta gttggagaac ctttaagacc taaattagaa
    23761 gggtcaactt ctatatcttt tacagtccat gtttctacta catcattttc gaaagcatca
    23821 tatattcttc caactttcat gtatcttggt gtgttcatat ctttaagagt tgttataagg
    23881 catggcattt taacttttaa gtcatggcaa caatcttcga attgtctttt tactaataca
    23941 tattcacctt cagtttttat ttcttcagca tatgttattg atggaagatt taaatgttca
    24001 gctatttgtg gtccaacttg tgcagtatct ccatctatag cctgtcttcc agctattata
    24061 atatcaaaat ctatattttt taatgctcct gctaatgctg atgaagttgc ccaagtatca
    24121 gcacctgcaa atgctctatc tgttaataat attcctctat ctgcacccat tgctaaagct
    24181 tcttttaaag ccatatctgc ttgaggaggt cccattgtta taacagttac atgagcaccc
    24241 atttcttctt ttaattttat agcttcttct aaacctgctt tatcatcagg gtttattata
    24301 cttggtactc catctctaat taaagtacct gtattaggat ctagtttaac ttctgttgta
    24361 tctggaactt gttttataca aacgactata ttcataaatc cttcctccat atctatttta
    24421 atatcgacct attgtgtata ttttaatatt tatctatttt aataattttc ctgaaataac
    24481 cattctttga acttcactag ttccttcata tatttcagtt atcttagcat ctctcatcat
    24541 tctttctact ggatagtcac gagtgtatcc atatcctcca tgaagttgta cagcttttgt
    24601 agtaacttcc atagctgttt cagctgcaaa taattttgcc attgctgctt ctactccata
    24661 aggttttcct aagtctttat ttatagctgc ttgatataca agatgtctcg ccgcttgtac
    24721 tttaacttcc atgtcagcta attggaattg tgtattttgg aattttgata atggtctacc
    24781 aaattgtact ctttctttta catatttaac agtttcatca agagcacctt gtgctaaacc
    24841 taaagcttgt gcagctatac caattctacc accatcaaga gtagacattg ctatcttaaa
    24901 tccttgacct tcttttccaa gtaaattttc tttaggtatt ctgcaatcct caaatattaa
    24961 ctcactagta gctgaacctc ttatacccat tttcttttct ttaactccaa agctaaaccc
    25021 aggagttcct ttttcaacta taaatgctga tattcctttg ttccccttag atttatcagt
    25081 cattgccatt actacatata tgtcaccagc tattgcattt gttataaata tttttgagcc
    25141 attaagtatg tattcatccc cgtctaaaac agcagttgtt tgttggccag acgcatctgt
    25201 accagcatta ggctcagtaa gaccaaatgc tcctaatttt tctccacttg ctagtggtct
    25261 taagaatttt tgtttttgtt cttcattacc atattgatat ataggccatg agccaagaga
    25321 tgtatgagct gataatataa ctcctgtagt accacaaact ctagacaatt cttcaactgc
    25381 cattatatat cctacagtgt ctccaccttc tccaccatat tcttttggat acggtatacc
    25441 catcattcct gcttttgcca tcttttccac tgtttcataa ggaaatcttt cttcttcatc
    25501 aagttctgtt gctaaaggtt taacttcatt ttcagcgaag cttacatata actctttaag
    25561 catctgatat tttttagaat ttaaatccat aatagcctcc tcagtatttt ataaatattt
    25621 tattttcatt tattaattaa aatctcggca acatctataa taaggtattt gaatatacgt
    25681 cgagaaattt ataaccttaa ttaaactacg ctctatattt atatgtgatt atactaacca
    25741 attatgacat taatttaatc ataaattgtt aatttaaaat caactatact taaaaaaaat
    25801 ctcgcttttt ggaattttgt gaaattttaa gttagaataa gatttctttc acactggtta
    25861 cagataaaat aatttgttta cgatatttat attagggtat attaaacatt aaatattgtg
    25921 tgtatatctg ttttttagcc atgtacagaa gatttttatc gtataaaaat tattttaata
    25981 gagctaaata atcgtatttt tatctgacta ttttttattt ttaaatatct tacatctata
    26041 atagtatcac tcaaaggata atttttcaat aatttctaat tattttttta acaatattta
    26101 ttacattttt tccacttttt tgtcattttt ttattgtttt gaaaaaaaat cagaattttt
    26161 taactttatt ttggattatt agtttaaatt agattttctt cttagattta taaatattta
    26221 cacaaaaaaa ctacctctgt atagattgta tacagagata gttttttact aattcctact
    26281 atagtttact atattttttt accaattctt acaaacatct acccattttt cacacttaga
    26341 aaacttctcc atttcttcac atgtaagttc tattgcagag ttactactgc cacatgcagg
    26401 aaatactgaa tcaaatctct tcattgaatc atctaaatac acttttagag aattttttaa
    26461 accaaatgga cagactccac caatagcatg tccagtaaac tccaagactt cttctggcgt
    26521 caacatttta gccttacaat taaattcagc cttatacttc ttattatcaa tcttagcatc
    26581 accagctgtc acaattaaaa ttgcatcatc tcctattttg aatgagagtg tcttagctat
    26641 tcttgctgga ataaccccag ctgcttcagc tgcaagttcc acagtagcac tagattgttc
    26701 aaattctaat atactgtctt ccttgccaaa ttgtttaaaa tactctctta cattgcttat
    26761 tgacattaaa tatccctcct agtctgaact tttataaatt tcatattatt cttaattata
    26821 tctataattg atttattttt caaattattc cataatatta gcattccatg tatcattatt
    26881 ttttccaact actagataaa gtccttttgc cattggaata ttgacaatat attctccctt
    26941 atattctaca ctcataccgg attcattaaa gtacctataa tcaaaattta gtccttttgg
    27001 aattacaaaa tcaacataac cttgattatt tggttttatt ccatatttca aagtcctttc
    27061 agttatagaa tttttttcat taaataaatc tatatttaca tatgcagaac acttatttat
    27121 tcccttttta ttttccacac aattttcaaa cttcatttta gcactgttta atttataact
    27181 atatttacta ttattgctta ttttaaatct tacaatagta ccaatatctc tacttctcct
    27241 gctatcaaaa tcaaaaacaa tttcctctaa atgatttatt tcataatctt tgacacttac
    27301 catatctcta tatactaatt tatttatagc tatgaatagt gtaaaaatcc ccaaaataaa
    27361 aaatgaaact tttataaact tattcatttt tagtctcctt atattttg
//
LOCUS       DAEPXF010000035        27348 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain HB27
            SAMN13376389-rid12479423.denovo.037, whole genome shotgun sequence.
ACCESSION   DAEPXF010000035 DAEPXF010000000
VERSION     DAEPXF010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13376389
            Sequence Read Archive: SRR10518432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 27348)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27348)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001069624.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001069624.1
            Genome Coverage       :: 315x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/15/2021 01:54:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,165
            CDSs (total)                      :: 4,076
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 89
            rRNAs                             :: 10, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 10 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 19 of 69
            Pseudo Genes (incomplete)         :: 44 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 10 of 69
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27348
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13376389-rid12479423.denovo.037"
                     /strain="HB27"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="China: Hebei"
                     /collection_date="2017"
     gene            264..797
                     /gene="pyrR"
                     /locus_tag="KRL67_001333"
     CDS             264..797
                     /gene="pyrR"
                     /locus_tag="KRL67_001333"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001156491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HBH1522228.1"
                     /translation="MVEKAQLMDEKAIARAITRISHEIIERNKGVENLVLVGIKTRGV
                     PIANRISKKIEQIEGTKVDTGDIDITLYRDDLEKIHVEPVVKGTYLDFNVNDKTVVLV
                     DDVLYTGRTVRASLDAIIDIGRPKSIQLAVLVDRGHRELPIRADYVGKNVPTSRHEII
                     SVSLLEIDGEDSVTIKE"
     gene            1081..2292
                     /locus_tag="KRL67_001334"
     CDS             1081..2292
                     /locus_tag="KRL67_001334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HBH1522229.1"
                     /translation="MKKTIMSLQHLLAMFGATVLVPILTGFNPSVAIFCAGVGTLIFH
                     FCTEGKVPAFLGSSFAFIPVILAAKEAYGGDLAYAQGGIIVAGLIYIIMSIIVKIVGV
                     NKIKLYFPAQVTGAMIVVIGLNLLPTAFSMASANFVIAFITLAIAILTNKFGRGFIKQ
                     LGILIAVFSGYIICLLLKLVDITTITEASLFAIPNFTVPKFSLGAIVIISPVVLAVFM
                     EHIGDMTTNGAVVGKNFIENPGLNRTLLGDGFATIVAGCLGGPANTTYGENTAVLAIT
                     KNYDPSILRRTAIFAILLACVGKFGGFLQSIPGSVMGGISIMLFSMITYVGLKTIRDS
                     SCVESKINILIIAVILLIGLGTTYLSNKGISIGIPITSTVKITGLSLAAIVGIVLNRI
                     LNNQDFKVEEE"
     gene            2591..4243
                     /gene="abc-f"
                     /locus_tag="KRL67_001335"
     CDS             2591..4243
                     /gene="abc-f"
                     /locus_tag="KRL67_001335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein"
                     /protein_id="HBH1522230.1"
                     /translation="MLLVKVENLKKYYADKLILDIDKLEILENDKIGLVGSNGQGKTT
                     LLKAILGEIEIDEGYTYLTGSYSYISQSENNIEACSHSKEKSLLNAPDKFEEYLSGGE
                     KVKLKIADALSNKKNIIIADEPTSNLDKKSIGVLEDMFKRHEGALLLISHDRRFLDEL
                     CTTILELEDGKLKAYKGNYTDYLMQKDEEVKRADFEYQEYVKEKKRLEKALLYKKALS
                     DGIRKTPKRMGNSEARLHKMGGQTNKKKLDSNVKAIKSRIDKLEVKNKPKVSKEINIK
                     IQDGMEIISKNLVEVKDMTLKLENKLLLDNVSFKIKRGKKIALLGDNGCGKSTLIKEI
                     LADKNDNIKINDKVKVGYFDQNQSLLDEEKSVLYNTKVNSSFDESFIRINLSLFGFKG
                     DDVYKKVKVLSGGEKVKIALCKIILEDNNFLVFDEPTNYLDIKSMEALEKALINTDKT
                     MLIVSHDRVFVSHICNYIIEIKDAKIREFDCNYEEYIINRNKKTPSRENQTKKENLLV
                     LENRLTTVISMLSIEKDNLKKELYESEYNELLKQISKLKNSF"
     gene            complement(4447..6222)
                     /locus_tag="KRL67_001336"
     CDS             complement(4447..6222)
                     /locus_tag="KRL67_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NFACT family protein"
                     /protein_id="HBH1522231.1"
                     /translation="MALDGLVIHSIVNELSSKLTGGKIDKIHQPEDDEVIFNIRNNKE
                     NFRLVLSASASNPRVYLTSNYQKENPLKAPMFCMLLRKYIQGGNIVEISQIGFERIIK
                     ISVESLDELKEKTVKNIMIEIMGRHSNIIITHGEENKIIDSIKRVPFSISRVRQVLPG
                     HDYSLPPEQNKLNPLDDISKDLFIKNLEESEGPIFKSIYSKFLGISPIIAKEICYRAG
                     VNQNTIIKDISDEQFDSLHKVFCNLFNDINSNKYSPCIIIDKKVDKVVDFSCINLTLF
                     SDLSYINKDSMSRILEDFYRTKDIKDRINQRSSDLKKSISVKLDRLYNKLKKQEEELS
                     ESENADIYKIKGELITSYIYMVEKGMESIKVANFYDENCNDVTIELNKNLTPSENAQK
                     YFKKYNKMKHAKVEISHQISLNKEEIDYLENIILSIENCENLAELQDIKEELAKVGYI
                     KTQKKNSKKDTIPSTKPHEFVSSDGFKILVGKNNKQNDYLTLRLADNDDLWMHTKNIP
                     GSHVIIKCAGKEVPDNTVFEGAMLAAFFSKSKLSSQVPVDYTKRKNVKKPSGSKPGMV
                     IYETNSTIYVTPEEETVAKLKVKSE"
     gene            6518..7552
                     /gene="ltaE"
                     /locus_tag="KRL67_001337"
     CDS             6518..7552
                     /gene="ltaE"
                     /locus_tag="KRL67_001337"
                     /EC_number="4.1.2.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low-specificity L-threonine aldolase"
                     /protein_id="HBH1522232.1"
                     /translation="MRFIDLRSDTVTMPTDEMREAMAKAPVGDSIFRDDPTVNKLEEL
                     AAAKVGKEDAIFLPSGTFGNQLALFTHCLRGQEIIIGKGYHIVTHEVGAPAVIAGVQL
                     RTIDEDESGALNPELVEKAIRRDDIHEPQTGLICVENAYSGGTVVSLDNLREIKKIAE
                     KHNLPVHLDGARLFNAALTLGVEAKEIAKCCDSVMFCVSKGLAAPMGSILAGSKEFIA
                     KARRKQKVMGGGMRQVGIVAAAGIIAIEKMTLRLNEDHENAKYLACELNKIDGIEVNN
                     VNPDISMVFFKMSEDIIKEDVLIKEFFERNIKINKMENGEYRFVTHVDITKEDLDYVL
                     KTLKELIGVC"
     gene            7839..8669
                     /locus_tag="KRL67_001338"
     CDS             7839..8669
                     /locus_tag="KRL67_001338"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HBH1522233.1"
                     /translation="MKFWKLHGIGNDFIAIDGRFDQINPSDYTALAKKVCHRRFSVGA
                     DGLLVVKDSKVADVEMVYYNSDGSRAAMCGNGLRCFCKFVYDNCIVKKESFTVDTLDG
                     IKDIKINLTNREINSIRVNMGKGSFIAKDVPVLTNKERFVQESIKVLDREFKVTSMLM
                     GVPHTVVIVDELDVDDVCKYGKEIENNKIFPEKTNVNFVKVEDKYNIHVYTWERGCGY
                     TLGCGTGMTASAIVCNLLNMVESSVNVTSQGGTVKIDVGDFSYMTGPAVKICEGVLEV
                     "
     gene            8673..9554
                     /locus_tag="KRL67_001339"
     CDS             8673..9554
                     /locus_tag="KRL67_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HBH1522234.1"
                     /translation="MAISMTGFGRGEYKDDNYYFLVECKTINHKYSDINIRLPRKISF
                     LEDKVRNLVKNYVKRGRVDLYIKFDLLGKEDVNLNFDEGLASQYIDILKEIKNKFDII
                     DDISVMNVARFPDIVKIEEKEEDEDLLWSMLNQAVEDALIKLREMRSEEGKKLAEDIA
                     MRCDLLKNHIEEIEKYSSSVVEDYREKLNLRISELLDDPSIIDENRLAQEVAIYADKS
                     SITEEIVRFKSHIGQLKNTIFKDDSIGRKIDFLIQEMNRETNTIGSKSSDINITNLVV
                     EVKSELEKIREQIQNIE"
     gene            9584..10201
                     /gene="gmk"
                     /locus_tag="KRL67_001340"
     CDS             9584..10201
                     /gene="gmk"
                     /locus_tag="KRL67_001340"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HBH1522235.1"
                     /translation="MFVKKGLLLVVSGPSGAGKGTICKELLKENDTIKLSVSATTRKP
                     RTGEVDGVNYFFISKEKFEEMIEKGEFLEYAQIYDNFYGTPKAAIMECLEKGQDVLLE
                     IEMQGAKQIKEVCPEGVFIFVLPPSLEELKNRIVGRGTETEAEIEKRFSCAYEEIKMI
                     KDYDYFIFNEDVKTSAKEIEGIISSEKNKVSRYKNIIIEKFKEEL"
     gene            10204..10470
                     /locus_tag="KRL67_001341"
     CDS             10204..10470
                     /locus_tag="KRL67_001341"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HBH1522236.1"
                     /translation="MLKPSINEVLEKIDNRYYLVGTVSKRARKLIDGEEPYVSNKTKE
                     KPVCVATKEVASGKITYRLLTEEEIEIEEARHHAEQHQQISEEE"
     gene            10475..11674
                     /gene="coaBC"
                     /locus_tag="KRL67_001342"
     CDS             10475..11674
                     /gene="coaBC"
                     /locus_tag="KRL67_001342"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HBH1522237.1"
                     /translation="MLKDKTVVIGISGGIAAYKACDVVSKLKKLNANIHVIMTKSATE
                     FVKPLTFQSLSQNYVVEDMFEEPKTWEVEHISLAKKADLFLIVPASANVIGKIANGIA
                     DDMLTTTVMATKAQVLIAPAMNTNMYENPIVQKNIQTLKSFGYKFIEPESGRLACGDI
                     GAGKLANPDIIVEEVLTNLTKEQDLKGQSMIVTAGPTVESIDPVRYLTNRSSGKMGYS
                     IAREAIARGADVTLISGPTNLSIPQNLKKFVQIESAQDLYEAVVSNLSENKIVIQSAA
                     VADYKPKTYSDKKIKKKNDDLSIELSRNYDIAYEIGKIKEDKILVGFAAETNDLIEHA
                     KGKIKKKNLDFIVANDLTKEGAGFRADTNIVKIIDVEGNITEYPKMKKEEVANVILDK
                     VKALLAK"
     gene            11913..14438
                     /gene="priA"
                     /locus_tag="KRL67_001343"
     CDS             11913..14438
                     /gene="priA"
                     /locus_tag="KRL67_001343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HBH1522238.1"
                     /translation="MNNILNLSRGFIMKKYAKVIVRSNTIYTDNLFTYQIPVFLSDVI
                     KIGHRILVPFGKGNKPTEAFVFQFTDSLDEKIKIKEIIDILDENPIFRKEDLELVYWM
                     KNRYLCTYIECINLIYPKGYKLNNYKVVLLGESLSGLNDIELKEKISTLSDKNREIVE
                     KVIDSKGKIKVDKLKYIPNLNSSLYTMNKNGIIKLCWEYKNHKNEKKVCYISLSLESD
                     KIDDYIEQNKINVGSKQKEILSFLKNNENVEINDLLDLLNASKQSINSLSKKKLITLE
                     FKDYYREPKSIYKSVLKRIKLNNEQQEAVDEIKSNMFVDDKKPYLIHGVTGSGKTEVY
                     MEIIEFALNQGLDSIFLVPEIALTPQTIDRLKSRFGDLVGVFHSKLSEGEKHDVYKAV
                     KAGKVRVLIGARSALFAPFNSLGLIIIDECHESSYKSEKNPKFNAIEVARFMALKNNI
                     TLILGSATPSIEEYYRAKSGEYKLINIKSRANDKPLPNIEVVDMKDELDKGNRSIFSL
                     KLQKEIRYAIEENNQVILFLNRRGYANFVSCRKCGYVFQCENCDISLTYHKKSNTGRC
                     HYCGYEKEIPKECPECKSTYVKPFGVGTQKIEEELKYIFPDIKTLRMDKDTTSKKGAL
                     DEILNKFKDKEVDVLIGTQMLSKGLDFENVTLVGILSADMILNFPDFKSFETTFQLIT
                     QVSGRAGRADKEGKVVLQTYDTEHYAIKHAIEYDYEGFYEDEIKIRKAFGYSPFNNML
                     SVVVSGEDERLVIKNIKNMHASLIYLLEKRGINDLGFILGPNPCSISKINQNYRWQIL
                     FKDENIEINLLKGIIKYICITKRDLIFDKNINVSIDINPNSVL"
     gene            14456..14896
                     /gene="def"
                     /locus_tag="KRL67_001344"
     CDS             14456..14896
                     /gene="def"
                     /locus_tag="KRL67_001344"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HBH1522239.1"
                     /translation="MALRQIVQIGEPVLRKKSKKVEKIDEKIIQLLDDMAETMYDADG
                     VGLAAPQVGILKRVVVIDIGEELIELINPEIIETSGEQIDEEGCLSVVGEAGNVRRPN
                     YVKVRALNRNGETIELEGEELLARAFCHEIDHLDGILFVDKIEK"
     gene            14911..15840
                     /locus_tag="KRL67_001345"
     CDS             14911..15840
                     /locus_tag="KRL67_001345"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HBH1522240.1"
                     /translation="MKIVFMGTPDIAVPCLQKIIDEKYEILGVVTQPDKPKGRGKKLG
                     MSPVKELAIENNIPVYQPVKARDKEFIDKIKSLNPDVIVVVAFGQILPKEILEIPKLG
                     CINVHVSLLPKYRGAAPINWVIINGEEKTGVTTMYMDEGLDTGDMILKTEVNLDENIT
                     AGELHDKMMNIGAETLKETLRLIEEGNAPREVQNHEEFSYAPIMNKSLGNIDFSKSAR
                     EIHNLVRGVNPWPSAYTTYNDVIMKVWKTKVLDEKSTKDVGTIIDVSKDGIKVSTIDN
                     VLLIEEIQMPNKKRMLVGEYIKGNTIETGLVLG"
     gene            15850..16602
                     /locus_tag="KRL67_001346"
     CDS             15850..16602
                     /locus_tag="KRL67_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF116 domain-containing protein"
                     /protein_id="HBH1522241.1"
                     /translation="MTDIKKYLTSVGILILILGILIGVVNFFIISLTQIFSLMINIII
                     ISLILIILCSTAVTYRVIKGKCVNSNIMKINFNIVSGLFPIISFIASSFGMSKSEIRR
                     IYIKLNNEYIYSNKYNFNPEDIIILIPHCIQENSCKLKVTNDIDNCKECGRCNVGELI
                     KLKEKTNVKIFVATGGTLARKIIMDNKPKAVVAVACERDLTSGIQDIKKIPVLGVFNK
                     RPNGPCVNTNVDMMDIEKAIGFLTGKNILVCN"
     gene            16671..17372
                     /locus_tag="KRL67_001347"
     CDS             16671..17372
                     /locus_tag="KRL67_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HBH1522242.1"
                     /translation="MYPVYGGFWGFDPTMVVLIPAILLTIYAQFKVSSTTNKYLRVNT
                     RRGYTGEQTARRVLDSNGLYDVKIEMVRGHLSDHYDPRRKAVRLSEDVYYGTSITSVA
                     VAAHECGHAIQHAKGYAPLQIRSSLVPVVNFASSISWFLIFLGFIMAGPFLKIGILLF
                     SASVLFQIITLPVEFNASSRAIVQLGNLGIIDESESRQSRRVLSAAALTYVAAALVSI
                     LQLLRLLLIAQRRND"
     gene            17434..18759
                     /gene="rsmB"
                     /locus_tag="KRL67_001348"
     CDS             17434..18759
                     /gene="rsmB"
                     /locus_tag="KRL67_001348"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HBH1522243.1"
                     /translation="MDAREVGFKVLCDIEKNDNYSNIAINKHFKNLEISDMDRGLATE
                     LIYGVIENKYYLDYIINKLSKIKVKKMSTYVKIFLRMGTYQILFLDSISDYAAVNETV
                     KLSKKYDKKSSGFINAILRNEIRAKETIMDITEEDSVKYLSIKYSYNSWIIKNWIDKF
                     GQEFAEDLLEANNEKPSIYIRTNTLKISREELIEKLSEMGIVCLKVPMVEEAIKVEKM
                     KNIEKNELFKAGLFTIQDISSMIVGKVINPKEGSFVLDVCSAPGGKSTHLATLMNNTG
                     QVVARDIFEHKLKLIKSTVNRLGLKNVFVERFDASEIDENSISKFDYVLADVPCSGFG
                     IIRRKPEIKYKKEEELKDITSIQKKILENASKYVKVGGTLVYSTCTIQDVENINIVTS
                     FVEENHNFEFVPIDTVNVDLDNQDKGYLKIYPNIHGIDGFFIAKLKRIR"
     gene            18767..19798
                     /gene="rlmN"
                     /locus_tag="KRL67_001349"
     CDS             18767..19798
                     /gene="rlmN"
                     /locus_tag="KRL67_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HBH1522244.1"
                     /translation="MEKKKIVLKNFTEDELKEFMKTIDEKPFRGSQIFSWIYKGAKTF
                     DDMNNIPKSLRNKLEEVSCIGHIDIELKLESKVDNTKKYLFLLDDGNIIETVMMDYDS
                     RVTVCVSNQVGCRMGCNFCASTMDGLIRNLEPWEILDQVIKIQEDTGKRVSNLVLMGS
                     GEPLDNFENTKQFLKIINEKNGLNIGYRHITLSTCGIVPKMYELADLEIAINLALSLH
                     SPYDEERRKIMPVANAYSIEEILNACRYYIKKTNRRVTFEYSLIKGVNDSEKEAKALA
                     KLLKGMLCHVNLIPINKVEEREYEKPDKAFIYKFRDSLEKNNIPATVRMSMGSDISGA
                     CGQLRRKYK"
     gene            19818..20570
                     /locus_tag="KRL67_001350"
     CDS             19818..20570
                     /locus_tag="KRL67_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HBH1522245.1"
                     /translation="MVYSCASHIGKIRKNNEDYCEGEVIDTEHGPIGIFAIADGMGGH
                     KKGEVASKLAVENIIDFLKENLLQHDNVKIDYIDDILKQAYNNVNSIVHKKSMEDIEF
                     EGMGTTLVTAIVYNNVLYVANVGDSRCYLLTDEKFDKITIDHSVVEELMMAHVITEEE
                     ARRHPQRNRITRAIGTDDMVVVDIFKKEIKKSDIILLATDGLTGFIDDEDIRDLILDY
                     EYERTSNISEELISMANDVSGKDNVSVIVIKV"
     gene            20587..22602
                     /gene="pknB"
                     /locus_tag="KRL67_001351"
     CDS             20587..22602
                     /gene="pknB"
                     /locus_tag="KRL67_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stk1 family PASTA domain-containing Ser/Thr
                     kinase"
                     /protein_id="HBH1522246.1"
                     /translation="MGDTILGNRYEIIRKIGDGGMAFVYEAKDRLLNRTVALKVLRPE
                     FVDDDEFLTKFKREAEAVASLSHPNIVNVYDVGEDGKVHYIVMEFVDGKNLKEIIQDE
                     GILDEYTALDITKQIAMALSAAHKKGIIHRDIKPHNILISNEGRVVKVADFGIAKAVS
                     NSTMTNIGSIIGSVHYFSPEQAKGKFVTNNADLYSLGIVLYEMLIGKVPFRGDSPISI
                     ALQHINDDIDFTSEEKVRIPQSVRTTIKKLTEKSSADRYQTAEELIEDIEYIEKNIDL
                     DFIKEYDDFATKKIDEKEINKVVNPTLAKPALEKVVKPVEVADLDDDEDYYDDFYEED
                     DDEDEEEEEIMRAKKNQRPKSTPSKRTKKKKKKQESPKSRRRLKVIAAVLILILCAQV
                     FLAYKFLFAGGFGNKSLTVPNLVNMTLEEAQSAVEKEGLYLSVKSEEYNSEVDENCII
                     SQTPEGGSTNVKKGDTINVVVSKGSSQASVPNVVGLTLSNAKQLIEENNLKVGTVKYE
                     YSSIYKEGTVLNQSPGAGSSRAQEGDEVNLYVSKGSEKSNTQTPTVPDKKPTTPENNT
                     PTEPDSNSGNSGGNSGSSNSGNSGGNSGNNGGNSGGSNSGNSGGSSGGSNSGDNGGSS
                     GGSNSGDNGGSSGGSNSGDNGDSSGGSNSGDNGGTSPGTGANIGKTE"
     gene            22710..23447
                     /locus_tag="KRL67_001352"
     CDS             22710..23447
                     /locus_tag="KRL67_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HBH1522247.1"
                     /translation="MKKKTVLITGGARGIGKAMSKAFAKEGYNVLVNFNKSENGAKEL
                     YTILNEKNFSVKLFKADISNREDVEDMVDYCIKEFGGLDVLVNNAGVSQDKLFTDITD
                     EDWDNMMNINLKGSFYCSQVALKYMISEKKGNIINISSIWGISGASCEVHYSITKAGI
                     IGMTKALAKEVGPSNIRVNSIAPGVINTDMLSGYNEEDIDVLVEETPLMRLGTPEDIA
                     NCAIFLASDKSNFITGQVISPNGGFVI"
     gene            23545..24429
                     /gene="rsgA"
                     /locus_tag="KRL67_001353"
     CDS             23545..24429
                     /gene="rsgA"
                     /locus_tag="KRL67_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HBH1522248.1"
                     /translation="MLEGKIIKGISGFYYVDTDNGIYECKARGIFRKQKITPLVGDRV
                     KISIVDEDEKKGILEEVDSRDTELIRPPIANVDKALIVFAIKNPKPNLSLLDRFIVLA
                     EKENLETVIILTKADLDDNDTLETVKNIYELSGYKVIPVSNITKLNIDKVKEELKENV
                     VVFAGPSGVGKSSLLNEIDENFKLQTGVVSDKIKRGKHTTRHAELLKLEFGGMVADTP
                     GFSSLALEDIEEVELKDYFIEFDKFNDCKFGSKCIHENEPNCAIKEAVANGEISKERY
                     DSYIQLLNEIRQNNSRRY"
     gene            24434..25105
                     /locus_tag="KRL67_001354"
     CDS             24434..25105
                     /locus_tag="KRL67_001354"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HBH1522249.1"
                     /translation="MIKLAPSILSADFAKLLEDVRKVESAGCEYLHIDVMDGHFVPNI
                     TLGPLVVKSLKKENINMVFDAHLMIENPDQYIEEFVKAGCDIITVHQEACVHLHRTIQ
                     NIKSHGIKAGVVLNPATPVDTIKHVLPDLDMVLLMSVNPGFGGQSFIPCVLDKIKELK
                     AIIDSQGLNIDIEVDGGISPKNVAEVVQAGANVIVAGSAIFGSDDIQETVNLFRKNAS
                     LEELV"
     gene            25114..25755
                     /locus_tag="KRL67_001355"
     CDS             25114..25755
                     /locus_tag="KRL67_001355"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HBH1522250.1"
                     /translation="MKICIVLNGEIEDYEVTRDIIIKECYDCIICADGGANHTYKMEI
                     MPDYILGDLDSVEEEKINFYKNKGVKFEKFPSKKDETDTELCLFLAKTLKVNHIDFFG
                     ALGGRIDHTLANIKLLYYLKEDGIYSRILSDKEEMYIVENEEISLYGNPGDTISVIAI
                     NGDAKGVTLTGLEYPLDNYYMKYSVPIGISNVMLSNSCKIKVEQGCVLVVRNL"
     gene            25886..26515
                     /locus_tag="KRL67_001356"
     CDS             25886..26515
                     /locus_tag="KRL67_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HBH1522251.1"
                     /translation="MVDSIIFDLDGTLWDSTEEVCKVWQDVLSKHKEIELSVTKDLFR
                     SLMGLSFEEIANRLFPNLSEEERMNLLDECSLKECDYLSENGAKLFDDIEDTLKLLSK
                     KYKLFIVSNCQAGYIESFLKAHKLEQYFIDFECPGNTGLHKGENNKLIIERNKLINPI
                     YVGDTQGDANSAKFVGIPFIYAKYGFGNVDVYDYFIDSFKDLLEHDILK"
     gene            26570..27226
                     /locus_tag="KRL67_001357"
     CDS             26570..27226
                     /locus_tag="KRL67_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HBH1522252.1"
                     /translation="MELQDTIFKRQSVRKFKNQDVLDEDILKMIKAAGAAPSGKNIQN
                     WHFVVIKRRDLMEKIADVITKKQQEILVEMDKVSVDKANRFRKFVKNFTLFYLKAPVL
                     VLVFTKVYNPSGYYELELIDAPKETIDKLFIRNPGMQSLGAAIENFTLSAIELGYGSC
                     WLTSQNYAADEIEAVLEAETGFEKGEYFLGAMLALGVPEDNLKSPSKKPVEEICTFIK
                     "
BASE COUNT        10901 a         2784 c         5097 g         8566 t
ORIGIN      
        1 ttaaaaaagc ttgaaaaatt tgaaatataa atatataatt tgagttgata gattaaaaaa
       61 ttatctttaa acgatacaga gatatcgtaa gacaattgct aaattacact gtatattttt
      121 tatataagag cttcttgtct tgtgatgaga agtttttttg ttgtttaaat tgaaatttta
      181 aataaaaata aatttataaa aaattaaaag aaaggagtag atttgtcaaa tagatatatt
      241 atatctttga caataattat gatatggtag aaaaagctca attaatggat gaaaaggcaa
      301 tagcaagagc aattactaga ataagtcatg aaataattga aagaaataaa ggtgtagaaa
      361 atctagtttt agttggaata aagactagag gtgtgccaat tgcaaataga atttctaaga
      421 aaatagaaca aatagaagga acaaaagtag atacaggaga tattgatata actttgtata
      481 gagatgattt ggaaaaaatt catgttgaac ctgtagttaa aggaacttat ttagatttta
      541 atgtaaatga taagacagta gttttagttg atgatgtttt atacactgga agaactgtga
      601 gagcttcact agatgctatt atagatatag gaagacctaa atcaatacaa ttagcagtgc
      661 tagtggacag agggcataga gagttgccta taagagcaga ttatgtagga aaaaatgtac
      721 ctacttctag acatgaaatt atatctgtca gtttattaga aattgatgga gaagattcag
      781 ttacaatcaa agaataatta tttttaagta ttatataaaa gcttaacatt gaatagtcag
      841 ggaggaggat aaagcttaca tagattgcac aaaataaata ttataatttt atggatgtga
      901 gtcttaatgt agtctttatt ctcctttatt tgagtttttg atgtcgtaaa atgtaaaata
      961 atgacaaatc atataataag tatatagagg ttaaaaaata gaatctaggg aatgataatt
     1021 aattaaaaga tattaattaa acagaggatt tatttatata taataatttt ggaggttttt
     1081 atgaaaaaaa cgataatgtc tttacaacat ctattagcca tgtttggagc gacagtatta
     1141 gttcccattt tgacaggttt taatccgtca gttgcaattt tctgtgctgg tgttggtaca
     1201 ttgatatttc atttttgtac agaaggaaaa gtacctgcct ttttaggttc aagttttgcg
     1261 tttataccag ttatactagc agccaaagag gcgtatggtg gagatttagc atatgctcaa
     1321 ggtggtataa tagtagcagg attaatttat attataatgt cgattatagt aaaaatagtt
     1381 ggtgtaaata aaattaaatt atattttcct gcacaagtta caggagctat gatagtagtt
     1441 ataggattaa atttattgcc aacagctttt agtatggctt cagccaattt tgtaatagca
     1501 tttataactt tagcaatagc aattttaacc aataagtttg gtagaggatt tataaaacaa
     1561 ttaggaatat taatagccgt tttttctggg tatataatct gtttactgtt aaagttagta
     1621 gatataacta cgattacaga agcaagttta tttgcaatac cgaactttac agttcctaag
     1681 ttcagtttag gagcaattgt gattatatct ccagttgtat tagctgtatt tatggaacat
     1741 ataggagata tgactactaa tggtgctgta gtaggtaaaa actttataga aaatcccgga
     1801 ttaaatagaa cattacttgg ggatggattt gcgactatag ttgcagggtg tttaggagga
     1861 ccagccaata caacttatgg agaaaataca gcagtacttg ctataactaa aaactatgac
     1921 ccttcaatac ttagacgtac agcaatattt gctatattgc ttgcatgtgt aggaaagttt
     1981 ggtggatttc ttcaaagtat accaggttca gttatgggtg gaattagtat aatgctattt
     2041 tcaatgataa cttatgtagg attaaaaact ataagggaca gttcttgtgt agaaagtaag
     2101 ataaatatat taataatagc agttatatta ttaataggtc ttggaacaac atatttatct
     2161 aataaaggca tttctattgg aatccctata acaagtacag taaagataac tggtcttagt
     2221 ttagctgcaa ttgttggaat tgtattaaac agaattttaa ataatcagga ttttaaagtt
     2281 gaagaagaat aaatttaaag aataaattgt atattgaata actaaaatta gttaattttc
     2341 tatagacatt tgtaattaat gttgatataa tgattaaaaa taaatgaaag aaagggcaag
     2401 gtaactggga caatgagaat ataatctatt ttttataatt taaagtattg taatttttga
     2461 aaagcctatt ttggtggctt tatttgcgat gcttaaatta aggagagtag attatagtct
     2521 atacgtttat ttgttatgcc tatttataga atctctcctt atatttaagt gtgttaagga
     2581 gggatttttt atgttgttag taaaagtaga gaatttaaag aaatattatg cagataaatt
     2641 aattttggat atagataaac ttgaaatatt agaaaatgac aaaataggtc tagttggttc
     2701 aaatggtcaa ggaaaaacaa cattattaaa agcaatattg ggtgaaatag aaattgatga
     2761 aggatatact taccttacag gaagctattc ttatataagt cagagtgaaa ataatattga
     2821 agcatgtagt catagtaagg agaagagtct tttaaatgct ccagataaat ttgaagaata
     2881 tttatcagga ggggaaaagg ttaagttaaa aatagcagat gcactgagta ataagaaaaa
     2941 tatcataata gctgatgagc caacttcaaa cttagataaa aaaagcattg gagttttgga
     3001 agatatgttt aaaaggcatg agggagcatt attattaata tctcatgata gacgttttct
     3061 ggatgagtta tgtacaacta tattagaact agaagatgga aaactaaaag cttacaaagg
     3121 caattacact gattatttaa tgcaaaaaga tgaagaagta aaaagagctg attttgagta
     3181 tcaagaatat gttaaagaga aaaaaaggct tgaaaaagct cttttatata aaaaagcttt
     3241 aagtgatggc ataagaaaaa caccaaaaag aatgggtaat tcagaagcta ggttgcacaa
     3301 aatgggtggt cagactaata agaaaaagtt ggactcaaat gtgaaggcta taaaaagcag
     3361 aattgataaa cttgaggtaa agaataaacc taaagtttct aaagaaataa acattaagat
     3421 tcaagatggt atggagataa ttagcaaaaa tctagtagag gtaaaagata tgactctaaa
     3481 gttagaaaat aagctcctgt tagataatgt ttcctttaag ataaaaagag gtaaaaagat
     3541 agcattatta ggtgataatg ggtgtgggaa aagtactctg ataaaagaaa tattggctga
     3601 taaaaatgac aatataaaaa taaatgacaa ggtaaaagta ggttattttg accaaaatca
     3661 aagtttatta gatgaggaaa agagtgttct atacaatact aaagttaata gttcatttga
     3721 tgaatccttt ataaggataa acttaagcct gtttggattt aaaggtgatg atgtctacaa
     3781 aaaagtaaaa gtacttagtg gtggagaaaa ggtaaaaata gcactatgca aaataatatt
     3841 ggaagataat aattttttag tatttgatga accaacaaat tacttagata taaaatctat
     3901 ggaagcatta gaaaaggcac tgataaatac tgataaaact atgcttatag tgtctcatga
     3961 tagagtattt gtatctcata tttgtaacta tatcatagag ataaaagatg ctaaaataag
     4021 ggagtttgat tgtaattatg aagaatatat aattaacaga aataaaaaaa ctcctagtag
     4081 agaaaaccaa accaaaaaag aaaatcttct agtattagaa aatagactta caactgttat
     4141 atctatgtta tctatagaaa aagataactt aaaaaaagaa ttgtacgaat cagaatacaa
     4201 tgaactacta aaacaaatca gtaagttaaa aaatagcttt tagtattaac tttttaatta
     4261 tatctatata agaagactga aaatatttgt tgggaatata agtatagtta ttaaaacaga
     4321 aaaatatttt attagaaaat taaaagtgag ctaattaaaa agtggtctta tgtacataag
     4381 gccacttttt aatttagaat gataatgtgt acttgtctga cacttatttt gtatttagtg
     4441 cagaatttat tcagatttaa ccttaagctt ggctactgtt tcttcttcag gagtaacata
     4501 aatagtactg ttagtttcat aaataaccat acctggttta gaaccactag gttttttcac
     4561 attttttctc ttagtatagt ctacaggcac ctgagaagaa agttttgact tactaaagaa
     4621 tgctgctagc atagcacctt caaatacagt attatcaggg acttccttac cagcacactt
     4681 tattattaca tgagaaccag gaatattctt tgtatgcatc cataagtcat cattatcagc
     4741 aagtcttagt gtaagatagt cattttgctt attgttttta ccaaccaata ttttaaatcc
     4801 atcagaagac acaaattcat gaggttttgt agatgggata gtatcttttt tactattttt
     4861 cttttgagtt ttgatatatc caaccttagc aagttcttct tttatatctt gtaattcagc
     4921 taaattttca cagttttcaa tacttaaaat tatgttttct aagtaatcta tttcttcctt
     4981 atttaaggaa atttgatgag atatctcgac tttagcatgt ttcattttgt tatatttttt
     5041 aaagtatttt tgagcatttt cagatggtgt caagttttta tttaattcta ttgttacatc
     5101 attacaattt tcatcataga agttagcaac tttaatactt tccatacctt tttctaccat
     5161 ataaatatat gaagtaatca attctccttt tatcttataa atgtctgcat tttcagattc
     5221 tgataattct tcttcttgtt ttttaagttt attataaagt ctatcaagct taacagatat
     5281 actcttcttt aaatcagatg aacgttgatt aattctatct ttaatatctt tagttctata
     5341 gaaatcttct agaatacgag acatactatc cttatttata tatgataaat cactaaataa
     5401 tgtaaggttt atacaactga aatcaactac cttatccacc tttttatcta taataatgca
     5461 aggtgagtat ttgttagaat ttatatcatt gaataaatta caaaatactt tgtgtaaaga
     5521 atcaaattgt tcatcagata tatcctttat gatagtattt tgatttacac ctgctcggta
     5581 gcaaatttct ttagctataa tagggcttat acctagaaat ttagaatata tagacttaaa
     5641 gataggtcct tccgattctt ctaggttttt aataaacaaa tccttagata tatcatcaag
     5701 agggttgagt ttgttttgtt ctggtggtaa tgaataatca tgaccaggta aaacttgacg
     5761 aactctactt atgctaaaag ggactctttt tatagaatct ataattttat tttcttcacc
     5821 atgagttatt attatgttgc tatgtcttcc cataatttca atcataatat ttttaactgt
     5881 cttttctttt aattcatcta aagattcaac acttattttt attattcttt caaaacctat
     5941 ttgagaaatt tctactatat ttccaccttg tatgtatttt ctaagtaaca tacaaaacat
     6001 aggcgctttt agagggtttt ctttttgata attacttgta agataaactc taggatttga
     6061 ggcacttgca cttaaaacca atctaaaatt ttctttatta tttcttatat taaaaattac
     6121 ctcatcatct tcaggttgat gtatcttatc tatttttcca cctgtaagtt ttgatgataa
     6181 ttcattgact attgaatgta taactaatcc atctaatgcc atttatagca cccctttctt
     6241 tgaccaaaat gagtcagtaa attaattata cacataatat acgctttgtg actaggggga
     6301 atttaaacaa tagagattaa tatttaaatt gtataattat ttatacttat tagaaaggaa
     6361 actttatttt taaaattaat aatttaaagt aggttaaata taaagtaaat atagtataat
     6421 tattgtgtag agtataagct ataaaagtat atgaacttta cagaaaaaat caattttaag
     6481 gttctatagt atataaatat gaaagaaggt tgaaaaaatg agatttatcg atttaagaag
     6541 tgatactgtt actatgccta ctgacgaaat gagagaagct atggcaaagg ctcctgttgg
     6601 agattcaatc tttagagatg accctactgt aaataaactt gaagaattag cagcagctaa
     6661 agttggaaaa gaagatgcta ttttccttcc aagtggaact tttgggaatc aattagctct
     6721 attcactcat tgcctaagag ggcaagaaat aattattggt aaaggttatc acattgtaac
     6781 tcatgaagtt ggggcacctg ctgtaatagc tggagttcag cttagaacta tagatgaaga
     6841 cgaaagtgga gctctaaatc ctgaacttgt tgaaaaggca attagaagag atgacataca
     6901 tgaaccacag acagggctta tatgtgtaga aaatgcatat tcaggtggaa ctgttgtgag
     6961 ccttgataat ttaagagaaa ttaaaaaaat agctgagaaa cataacttac cagtacattt
     7021 agatggagca agattattta acgcagcatt aactttgggt gtagaagcta aagaaatagc
     7081 aaagtgttgt gatagtgtta tgttctgtgt gtcaaaaggt ttagctgcac caatgggttc
     7141 tatacttgct ggaagtaaag aatttatagc aaaagctaga agaaagcaaa aagttatggg
     7201 tggaggaatg agacaagtag gtatagttgc agcagcaggt ataattgcca ttgaaaaaat
     7261 gactttaaga ttgaatgaag accatgaaaa tgcaaaatat ttagcttgtg aattaaataa
     7321 aattgatggc attgaagtaa ataatgttaa tccagatata agtatggtat tttttaagat
     7381 gagtgaagat attataaaag aggatgtttt aataaaagaa ttttttgaaa gaaatataaa
     7441 aattaataaa atggaaaatg gtgaatatag atttgtaact catgtagata ttacaaagga
     7501 agatttagac tatgttttaa aaacacttaa agaattaata ggtgtatgtt aatagatatc
     7561 tactaaaagg attatagctg gttaccagat gtttaattaa aaaattcata aacagtttaa
     7621 ataaaacata cataaaattc atatagaaat attaatatat aaacatagaa agaactagct
     7681 taaaaacata atataattac cctaattgta atggcaactc tccctcacat cctcgaatta
     7741 tagttgtcat tacaaaactt ttcccataaa ttataagttt atgggaattt ttttatgtta
     7801 taatagttta agtgttaatt tacgaaggag cgatacttat gaaattttgg aaactacatg
     7861 gaataggaaa tgattttata gcaatagatg gaagattcga ccagataaat ccatctgatt
     7921 atactgcttt agcaaaaaaa gtatgccata gaagattttc tgtaggagcg gatggattgt
     7981 tagttgtgaa agattcaaaa gttgcagatg tagaaatggt atattataat tctgatggtt
     8041 caagagcagc tatgtgtgga aatggcctta gatgtttttg taaatttgtt tatgataatt
     8101 gtatagttaa aaaagaaagt tttacagtag atacattaga tggtataaaa gatataaaaa
     8161 taaatcttac caatagagaa ataaactcta taagagtcaa tatgggaaaa ggaagtttta
     8221 tagctaagga cgttcctgta cttacaaata aagagcgttt tgtgcaagaa agtataaagg
     8281 ttttagatag ggagtttaaa gttacttcta tgttgatggg ggttcctcat acagtagtta
     8341 tagtagatga attagatgta gatgatgttt gtaaatatgg aaaagagatt gaaaataata
     8401 aaatatttcc tgaaaaaacg aatgtaaatt ttgtaaaggt agaagataag tataatatac
     8461 atgtttatac ttgggaaaga ggttgtggat atactctagg ttgtggtact ggtatgactg
     8521 catcagcaat agtgtgcaat ttacttaata tggtagaaag tagtgtaaat gtaacgtctc
     8581 aaggtggaac agttaaaata gatgtaggag atttctctta tatgacaggt ccagcagtaa
     8641 aaatatgtga aggtgttttg gaggtttaat taatggctat aagtatgact ggttttggaa
     8701 gaggagaata caaggatgat aattattact ttttggtaga gtgtaaaacg ataaatcata
     8761 agtattcaga tataaacata cgtcttccaa gaaaaatttc atttttagag gataaagtta
     8821 gaaatttagt aaaaaattat gtaaaaagag gaagagtaga tttatacata aagtttgact
     8881 tattaggcaa agaagatgta aacttaaatt ttgatgaggg attagcatct cagtatatag
     8941 acatactaaa agaaataaaa aataaatttg acattataga tgatattagc gttatgaatg
     9001 ttgcaaggtt tcctgatatt gtaaaaatag aagaaaagga agaagatgaa gatttattat
     9061 ggtcaatgtt gaatcaagca gttgaagatg cattaatcaa attaagagaa atgagaagtg
     9121 aagaaggtaa aaaattagct gaagatattg ccatgagatg tgatttatta aaaaatcata
     9181 tagaagagat tgaaaaatat tcaagtagtg ttgtagaaga ttatagagaa aaattgaatt
     9241 tgagaataag tgaattactg gatgacccaa gtataataga tgagaataga ttagcacaag
     9301 aagttgcaat atatgcagat aaaagcagta taacagaaga aatagttaga tttaaaagtc
     9361 atatagggca acttaaaaat actatattta aagacgactc tataggtaga aaaatagatt
     9421 tcttaataca agagatgaat agagaaacta acactatagg ttcgaagtca tctgatataa
     9481 atataactaa tttagtagta gaggtaaaaa gcgaattaga aaaaataagg gaacaaatac
     9541 aaaatataga gtaagaatac aatattagaa ggggaatgct taaatgtttg tgaaaaaagg
     9601 attattgcta gttgtatcag gaccatcagg tgcaggaaaa ggtactattt gcaaagaact
     9661 acttaaagaa aatgatacaa taaaactttc tgtatcagct actacaagaa aacccagaac
     9721 tggagaagtt gatggagtaa attatttctt cataagtaag gaaaagtttg aagagatgat
     9781 agaaaaaggt gagtttttgg agtatgctca aatttatgat aatttttatg gaactccaaa
     9841 agcagcaata atggaatgtt tagaaaaagg acaagatgta ttactggaga ttgaaatgca
     9901 aggagcaaaa caaataaaag aagtttgtcc agagggagta tttatatttg tactaccacc
     9961 atcacttgaa gaattaaaaa ataggattgt tggaagaggt acagaaacag aagctgaaat
    10021 agagaagaga tttagctgtg catatgaaga aattaaaatg attaaggatt atgattattt
    10081 tatatttaat gaagatgtaa aaacatctgc taaagaaata gaaggtataa tatcatctga
    10141 aaagaataaa gttagcagat ataaaaatat tataatagag aaatttaagg aggaattata
    10201 attatgttaa aaccatctat caatgaggta ctagagaaaa tagacaacag atactattta
    10261 gtagggactg tttcgaagag agcaagaaaa ttaattgatg gagaagaacc atatgtttca
    10321 aataaaacta aggagaagcc tgtatgtgta gcaactaaag aagttgcaag tggaaaaata
    10381 acatatagat tattgacaga agaggaaata gagatagaag aagctagaca tcatgctgag
    10441 caacaccaac aaatttcaga agaggagtaa ggtcatgcta aaagataaaa ctgttgtaat
    10501 tggcataagt ggcggtatag ctgcatataa ggcttgtgat gttgtaagta agttaaaaaa
    10561 actaaatgca aatatccatg ttataatgac aaaatcagca acagaatttg ttaaaccact
    10621 tacatttcaa tcactgagtc aaaattatgt agtagaagat atgtttgaag aaccaaaaac
    10681 ttgggaagtt gaacatatat cattagcaaa aaaagcagat ttattcttaa tagtgccagc
    10741 aagtgcaaat gttataggca agattgcaaa tggtatagct gacgatatgt tgacaactac
    10801 tgttatggca acaaaagcac aggttcttat agcaccagct atgaatacta atatgtatga
    10861 aaatccaatt gttcaaaaaa atatacaaac tttaaagagt tttggatata aatttattga
    10921 accagaaagt ggaagacttg catgtggaga tattggagca ggtaaactag ctaatccaga
    10981 tataatagta gaggaagttt taactaatct tactaaagag caagatttaa aaggtcaaag
    11041 tatgatagtt acagctggtc caactgtaga gagtatagac ccagtaagat atttaactaa
    11101 taggtcttct ggtaaaatgg gatattctat agcaagagaa gctatagcta gaggtgctga
    11161 tgtaacatta atatcaggtc ctacaaatct ttctatccca caaaatctta aaaaatttgt
    11221 tcaaatagag tcagcacaag atttgtacga agcagtagta tcaaatttaa gtgaaaataa
    11281 aatagtaatt caaagtgcag cagttgcaga ttataaacca aaaacttatt ctgacaaaaa
    11341 aattaaaaag aaaaatgatg atttatcaat agaactaagt agaaattatg atattgctta
    11401 tgaaatagga aaaataaaag aagataagat actagttgga tttgcagctg aaactaatga
    11461 ccttatagaa catgccaaag gaaaaataaa aaagaaaaat ttagatttta ttgtggcaaa
    11521 tgatttaact aaagaaggtg ctggttttag agctgatact aatattgtaa aaataataga
    11581 tgtagaggga aatattactg agtatcctaa gatgaaaaaa gaagaagtag ccaatgttat
    11641 tttagataaa gttaaagcgt tgcttgcgaa ataagacctt ttttaaatat gatatttgtg
    11701 tattttatca cagctaaagt ggctgaaagc tgcaatttaa ttttagcgag taaaaactcg
    11761 caaatgtcag ttaaattgta gttttcagca tttattttgt cataaagtat aaaatataat
    11821 taatagttaa ttattaaaat aaagttaata ttaataactt gaaattgctt ataaattctg
    11881 agattttaat atcttgtaaa gtatattaaa atatgaacaa tatattaaat ttaagtagag
    11941 gttttataat gaaaaaatat gcaaaggtaa tagttagaag taatactata tacacagata
    12001 atttatttac atatcaaatt cctgtatttt tatctgatgt aataaaaatc ggacacagaa
    12061 tattagtacc atttggaaaa ggtaataaac caacagaagc ctttgtattt caatttacag
    12121 atagcttaga tgaaaaaata aaaataaaag aaattattga tatattagat gaaaatccaa
    12181 tatttagaaa agaagattta gagttggttt attggatgaa aaatagatat ttgtgtactt
    12241 atatagaatg tataaatttg atatatccaa agggatacaa actaaataat tataaagtag
    12301 tcctattagg tgaaagttta agtggattaa atgatattga attgaaggaa aaaatatcaa
    12361 ctttaagtga taaaaataga gaaatagtag agaaagtgat tgatagtaaa ggtaaaatta
    12421 aggttgataa gttaaaatat ataccaaact taaatagttc tttgtacaca atgaataaaa
    12481 atggaattat aaaattatgt tgggaatata aaaatcacaa aaatgaaaag aaagtatgtt
    12541 atatatcttt gagtttagaa agtgataaga ttgacgatta tatagagcaa aataaaataa
    12601 atgtgggaag taaacaaaaa gagatattaa gttttcttaa aaataatgaa aatgtagaga
    12661 taaatgattt attggattta ttaaatgctt caaaacaaag tataaattct ctaagcaaga
    12721 aaaagctaat aaccctagaa tttaaggatt attatagaga gccaaaaagt atttacaaat
    12781 ccgtattaaa aaggataaaa ttaaacaatg agcaacaaga agcagttgat gagataaagt
    12841 cgaatatgtt tgtagatgat aaaaaaccat acttaattca tggagtaact ggaagtggaa
    12901 aaactgaagt ttacatggaa ataatagaat ttgctctaaa tcaaggctta gacagtatat
    12961 tcttagtacc tgagatagca ttaactcctc aaacaataga taggctaaag agtagatttg
    13021 gagatttagt tggagtgttc cacagtaaac tatctgaagg agaaaaacat gatgtttaca
    13081 aggctgtcaa agcaggcaaa gtaagagtgt taattggagc taggtcagct ttatttgcac
    13141 cttttaattc tttaggctta ataataattg atgaatgtca tgagtcatca tataagtctg
    13201 agaagaatcc aaaatttaat gctatagaag ttgctagatt tatggcatta aaaaataata
    13261 taactcttat tttaggttca gcaacaccct caattgaaga gtattatagg gcaaaatctg
    13321 gagaatacaa acttataaat ataaaaagta gagcaaatga taagcctctt ccaaacattg
    13381 aagtggtaga catgaaagat gagttagata aaggaaatag aagtatattt agtttgaaat
    13441 tacaaaaaga aatacgttat gcgattgaag aaaataatca agttatacta tttttaaata
    13501 gaagaggtta tgcaaatttt gtatcttgta gaaagtgtgg atatgtgttt caatgtgaaa
    13561 attgtgatat atctttaact tatcataaga aaagcaatac tggaagatgc cactattgtg
    13621 gatatgagaa agaaattcca aaagagtgtc ctgaatgtaa aagtacttat gttaagccat
    13681 ttggagttgg aactcaaaaa attgaagagg aattaaaata tatttttcca gatataaaaa
    13741 ctttgagaat ggataaagat acaacttcaa aaaaaggtgc tttagatgag atactaaata
    13801 aatttaaaga taaagaagta gatgtactga ttggaacaca gatgttaagt aaagggttgg
    13861 attttgagaa tgtaacttta gtaggtattt tgtctgcaga catgatatta aattttccag
    13921 actttaaaag ttttgaaact acatttcaat tgataactca agtctctgga agagcaggga
    13981 gagctgataa agagggaaaa gtagtacttc agacttatga cactgaacac tatgctatta
    14041 agcatgctat tgaatatgat tatgagggtt tctatgaaga tgagataaaa ataagaaaag
    14101 catttggata ctctcctttt aacaacatgt taagtgtagt tgttagtggt gaagatgaaa
    14161 gattagtgat aaaaaatata aaaaatatgc atgcatcatt gatttattta ttagaaaaaa
    14221 ggggtataaa tgatttagga tttatactag gtccaaatcc atgttcaata tcaaaaataa
    14281 atcaaaatta tagatggcaa atactcttta aagatgaaaa tattgaaatt aatctcttaa
    14341 agggtataat aaaatatata tgtataacaa aacgagattt gatatttgat aagaatatca
    14401 atgtatctat agatattaat ccaaacagtg tattataaat ttaggaggaa ttataatggc
    14461 tttaagacaa atagttcaaa taggagaacc agtattaaga aaaaaatcaa aaaaggttga
    14521 aaaaattgat gaaaagataa tacaattatt agatgatatg gcagaaacta tgtatgatgc
    14581 agatggtgtt ggactggctg cacctcaagt tggaatatta aaaagagtag tagtaataga
    14641 tattggagaa gagcttatag aacttataaa tcctgaaata atagaaacat ctggggagca
    14701 aatagatgag gaaggatgct taagtgttgt tggagaagcg ggaaatgtta gaagaccaaa
    14761 ttatgtaaag gttagagctt taaatagaaa tggtgaaaca atagagttag agggagaaga
    14821 acttttagct agagcatttt gtcatgagat agaccactta gatggaattt tatttgttga
    14881 caaaatagaa aagtagtagg agttgatttg atgaaaatag tatttatggg aactcctgat
    14941 atagcagttc catgtttaca aaaaataata gatgagaagt atgaaatatt gggtgttgta
    15001 actcaaccag ataaaccaaa aggaagaggt aaaaaactag gtatgagccc agtaaaagaa
    15061 ctggcaattg aaaataatat acctgtatat caaccagtaa aggctagaga taaagaattt
    15121 atagataaaa ttaaatcttt aaatccagat gtaatagtag ttgtagcttt tggacagata
    15181 cttccaaaag aaatattaga gattcctaaa cttggatgta taaatgttca tgtttcttta
    15241 cttccaaaat atagaggtgc ggcacctata aattgggtaa taataaatgg tgaagaaaag
    15301 actggtgtta caactatgta tatggatgaa ggattagata ctggagatat gatactaaag
    15361 acagaagtaa atcttgatga aaatataaca gcaggagaac ttcatgataa aatgatgaat
    15421 ataggagcag aaactttaaa agaaacatta agattaattg aagaaggaaa tgctccaaga
    15481 gaagtacaaa atcatgaaga attttcctat gcgccaataa tgaataaatc attaggtaac
    15541 atagattttt caaaaagtgc cagagaaata cacaatttag ttagaggagt aaatccatgg
    15601 ccaagtgctt atactaccta taatgatgtc ataatgaaag tttggaaaac taaagtgtta
    15661 gatgaaaaaa gcacaaaaga tgtaggtaca ataattgatg taagcaaaga tgggataaaa
    15721 gtaagtacta tagataatgt acttttaatt gaagaaattc agatgccgaa taaaaagaga
    15781 atgttggttg gtgaatatat aaaagggaat actatagaaa ctggtttagt attgggttag
    15841 gtgaaaagta tgacagatat aaagaagtat ttaacaagtg ttgggatttt gattttaata
    15901 ttaggaattt tgataggagt tgttaatttt tttataataa gtttaactca aatattctcc
    15961 ttgatgataa atattataat aatatcacta atacttataa tactatgttc aacagcagtt
    16021 acttatagag taattaaagg aaagtgtgtt aactcaaata taatgaaaat aaattttaat
    16081 attgtcagtg gattatttcc cattatatct ttcattgcat catcatttgg tatgtcaaaa
    16141 agtgagatta gaagaatata tataaagtta aataatgaat atatatacag taataaatat
    16201 aattttaatc cagaggatat aataatttta atacctcatt gtattcaaga gaatagttgt
    16261 aaattaaaag ttactaatga tatagataat tgtaaagaat gtggaagatg caacgtagga
    16321 gaattaatta agctaaagga aaaaactaat gtaaaaatat ttgtagctac aggaggaacc
    16381 ctagctagaa aaattataat ggataataaa cctaaagctg ttgtagctgt tgcatgtgaa
    16441 agagatttga catctggaat acaagatatt aaaaaaattc ctgttttagg tgtatttaac
    16501 aaaagaccta atggaccttg tgtaaatact aatgtagata tgatggatat tgaaaaagct
    16561 ataggctttt taacaggaaa aaatatatta gtttgtaatt aaactgtttt taagttaagt
    16621 gaatattaag aggatgtata ataaaaatat attttaggag agtgttattt atgtaccctg
    16681 tatatggcgg tttttgggga tttgacccaa caatggttgt attaattcca gctatattac
    16741 ttactatata tgcacaattt aaagtgagtt caacaacaaa taagtattta agagtaaata
    16801 cacgtcgagg atatactgga gagcaaacag caagaagagt acttgattca aatggtttgt
    16861 acgatgtaaa gatagaaatg gttagagggc atctaagtga ccattatgac ccaagaagaa
    16921 aggctgtaag gttatcagaa gatgtatatt atggaacatc tataacttct gttgcagtag
    16981 ctgcacatga gtgtggtcat gcaatacaac atgctaaagg ttatgcacct cttcaaataa
    17041 gaagtagttt agtaccagta gtgaattttg cttcaagtat atcttggttt ttaatatttc
    17101 taggatttat tatggctggt ccatttttaa aaattggtat actattgttt tcggcttcag
    17161 tgttattcca aataatcaca ttgcctgtag aatttaatgc ttcaagtaga gcaatcgttc
    17221 aacttggaaa tttaggtatt atagatgaga gtgagtcaag acaaagtaga agagtacttt
    17281 cagcagcagc tttaacatat gttgcagcag cattggtttc aatacttcaa ttacttagat
    17341 tgttactaat agctcaaaga agaaatgact aaaagtaaag tgggttgttt agaaacaacc
    17401 cttttttatg tttaataaaa gggagaaatt tatatggatg caagagaagt tggatttaaa
    17461 gtattatgtg atatagaaaa aaatgacaat tattcaaata ttgcaataaa taagcatttt
    17521 aaaaatttag aaataagtga tatggataga ggacttgcaa cagagttaat ttatggagta
    17581 atagaaaata aatattattt agattatata atcaataaac tttcaaaaat aaaggtaaaa
    17641 aaaatgtcaa cttatgttaa aatcttttta agaatgggaa catatcagat tttattttta
    17701 gatagcatat cagattatgc agcagttaat gaaactgtaa aattatctaa gaaatatgat
    17761 aaaaaatctt ctggtttcat aaatgctatt cttagaaatg aaataagagc taaagagact
    17821 ataatggaca taactgagga agatagtgta aaatacttat ctataaagta ttcctataac
    17881 tcttggatta taaaaaattg gatagataaa tttggacaag aatttgctga agatttgctg
    17941 gaagcgaata atgaaaagcc tagtatctat ataagaacga atacacttaa aataagtaga
    18001 gaagaactta ttgaaaaatt aagtgaaatg ggtattgtat gtttaaaagt tcctatggta
    18061 gaagaagcta ttaaagtcga aaaaatgaaa aatatagaaa aaaatgaatt atttaaggct
    18121 ggtttattta caattcaaga tataagttct atgatagtag ggaaagtaat taatccaaaa
    18181 gaaggttcat ttgttttgga tgtatgtagt gctcctggag ggaaatctac tcacttagct
    18241 actttaatga acaatactgg tcaagtagta gctagagata tttttgagca taaactaaag
    18301 cttataaagt ctactgtaaa tagattgggg cttaaaaatg tatttgtaga aagatttgat
    18361 gcttctgaga ttgatgaaaa tagtataagt aaatttgatt atgttttagc agatgttcct
    18421 tgctctggtt ttggtataat aagacgtaaa ccagaaatta agtataagaa agaagaagag
    18481 ttaaaagaca tcacttctat acaaaagaaa attttagaaa atgcatctaa atatgtcaaa
    18541 gttggtggaa ctttagtata tagtacatgt acaattcaag atgtggagaa tataaatata
    18601 gttacttctt ttgttgaaga aaatcataac tttgaatttg ttccgattga tacagtaaat
    18661 gtagacttag acaatcaaga taaaggatat ttaaagatat atcctaatat tcatggtata
    18721 gatggttttt tcatagcaaa attaaagaga ataaggtagt gagaaaatgg aaaagaaaaa
    18781 gatagtatta aaaaatttta ctgaagatga acttaaagaa tttatgaaaa ctatagatga
    18841 aaagcccttt agaggaagtc aaatattctc ttggatatat aagggtgcaa aaacttttga
    18901 tgatatgaat aatataccaa aaagtttgag aaataaatta gaagaagttt catgtatagg
    18961 gcatatagat atagaattaa agttagaatc taaggtagat aatacaaaaa aatatttgtt
    19021 tttattagat gatggaaata taatagaaac tgtgatgatg gattatgata gtagagttac
    19081 tgtctgtgtt tctaaccaag taggttgtag gatgggatgt aatttttgtg catctacaat
    19141 ggatggatta attagaaact tagagccatg ggaaatttta gaccaagtta ttaaaattca
    19201 agaagatact ggtaagaggg tatcaaatct tgtattgatg gggagtggtg agccacttga
    19261 taactttgaa aatacaaaac aatttttaaa aataattaac gaaaaaaatg gtcttaatat
    19321 agggtataga catataactc tttcaacttg tggaatagta cctaaaatgt atgagttggc
    19381 agatttagaa atagctataa atttagcttt atcacttcat tcaccatatg atgaagaacg
    19441 aagaaaaatt atgcctgtag caaatgctta ttctattgaa gaaatattga atgcatgtag
    19501 atattatata aaaaagacaa atagaagggt tacatttgaa tattctctta taaaaggggt
    19561 aaatgactca gaaaaagaag caaaggcatt agctaagctc ctaaaaggta tgctatgtca
    19621 tgtaaattta attccaataa ataaagttga agaaagagaa tacgaaaagc cagataaagc
    19681 atttatttat aaatttaggg atagtttgga gaagaacaat atacctgcca cagttaggat
    19741 gtctatgggt tctgatatta gtggagcttg tgggcaattg agaagaaaat acaagtaatt
    19801 tgtaaggagg aagtcgtatg gtttatagtt gtgcctccca tataggaaaa ataagaaaaa
    19861 acaatgaaga ctattgtgag ggagaagtta tagatacaga acatggtcca ataggaatat
    19921 ttgccatagc tgatggaatg ggtggacata aaaaaggtga agtcgccagt aaattagcag
    19981 tagaaaatat aattgatttt ctaaaggaaa acttattaca acatgataat gtaaaaatag
    20041 actatataga tgatatatta aaacaggctt ataataatgt aaactcaatt gtgcacaaaa
    20101 aatctatgga agatatagaa tttgaaggaa tgggaactac attagttacg gctatagtgt
    20161 ataataatgt cttatatgtg gcaaatgttg gagatagcag atgttatttg ttaacagatg
    20221 aaaaatttga taaaataact atagaccatt ccgtggttga agaacttatg atggcccatg
    20281 tcattacaga agaagaggca agaagacatc cacagaggaa tagaataact agagcaattg
    20341 gaacagatga tatggttgtt gtagatattt ttaagaaaga aatcaaaaaa agtgatataa
    20401 tacttcttgc gacagatgga ttaactgggt ttattgatga tgaagatata agagatttaa
    20461 tattagatta tgaatatgag agaactagta atataagtga agaattgata agtatggcca
    20521 atgatgtttc tggtaaagat aatgtttcgg ttatagtgat aaaagtataa ttaggacggt
    20581 gatattgtgg gagatacaat tttaggaaat cgatatgaaa tcatcaggaa gattggtgat
    20641 ggaggaatgg cctttgtata tgaggctaaa gacagattat taaacaggac tgttgcgctt
    20701 aaagtactta gacctgaatt tgtagatgat gatgaatttc taacaaagtt taaaagagag
    20761 gcagaagcag tagcaagtct ttcacatcca aatatagtaa atgtttatga tgttggtgaa
    20821 gatggaaagg ttcactacat tgtaatggag tttgtagatg ggaagaatct aaaagagatt
    20881 atacaagatg aaggtatatt agatgaatat actgcattag atataacaaa acaaatagct
    20941 atggcactta gtgccgcgca caaaaagggg attatacata gagatataaa acctcataat
    21001 atcctcatat cgaatgaagg aagagtagta aaagtggctg actttggtat agccaaagct
    21061 gtttcaaatt caacaatgac taatattggt agtataatag gttcagtgca ttatttttca
    21121 ccagagcaag ctaaaggtaa atttgtaact aataatgctg acctgtattc tctaggtata
    21181 gttttatatg aaatgttgat aggaaaagtt ccatttaggg gagatagtcc aatttctata
    21241 gcacttcaac acattaatga tgatatagac tttacatcag aagaaaaagt taggattcct
    21301 caaagtgtaa gaactacaat aaaaaagctt acagaaaaat ctagtgcaga tagatatcaa
    21361 actgctgaag aattgataga agatatagag tatatagaaa aaaatattga tttagatttt
    21421 ataaaagaat atgatgattt tgcaactaag aaaattgatg aaaaggaaat taataaagtt
    21481 gtaaatccaa cacttgcaaa accagctctt gaaaaagtgg ttaagcctgt tgaagtagct
    21541 gatttggatg atgatgaaga ttactatgat gatttttatg aagaagatga tgatgaagat
    21601 gaagaagagg aagagataat gagagctaaa aagaatcaga gacctaagag tactccaagt
    21661 aagagaacta agaaaaagaa gaaaaaacag gaaagcccaa aatctagaag aagattaaaa
    21721 gtaattgcgg ctgttttgat actgatattg tgtgctcaag tattcttagc atataagttt
    21781 ttatttgctg gagggtttgg caataaatct ttgactgttc ctaatcttgt aaatatgaca
    21841 ctagaagaag ctcaaagcgc tgttgaaaaa gaaggattat acctgagtgt aaaaagtgaa
    21901 gagtataata gtgaagttga tgaaaattgt ataatatcac agacacctga aggtggaagt
    21961 acaaatgtca aaaagggtga taccataaat gttgttgtaa gtaaaggttc aagtcaggca
    22021 tctgttccta atgtggtggg acttactttg agtaatgcaa aacaacttat tgaagaaaat
    22081 aacttaaaag tgggaactgt taaatatgaa tatagctcaa tatataaaga aggtacagtt
    22141 ttaaatcaga gtccaggtgc tggctcatca agagctcaag aaggtgatga ggtaaaccta
    22201 tatgtaagca aaggttcaga aaaatcaaat acacagactc caacagtacc agataaaaaa
    22261 ccaacgacac cagagaataa tacaccaaca gaacctgatt caaactcagg gaacagtggt
    22321 ggaaattcag gaagctcaaa ttcaggaaat agtggaggaa actcaggcaa taatggagga
    22381 aactcaggag gttcaaactc aggaaatagt ggaggaagct caggaggctc aaactcagga
    22441 gacaatggag gaagttcagg aggttcaaac tcaggagata atggaggaag ttcaggaggc
    22501 tcaaactcag gagacaatgg agatagctca ggaggctcaa actcaggaga taatggagga
    22561 acttcaccag gaactggcgc aaacattggt aaaacagaat aaatattaat aaaataagag
    22621 ctgtcctttt atagaaaata ttttctttaa agggcagttt gttttgtata aaatattgaa
    22681 ttggttataa tataaggaga aaagtattta tgaagaagaa aacagtttta attacaggag
    22741 gagctagagg gattggtaaa gctatgtcta aagcttttgc aaaagaaggt tacaatgtat
    22801 tagtaaattt taataagtca gaaaatggag ctaaagagtt gtatactata ttaaatgaaa
    22861 aaaatttttc tgtaaaactt tttaaggctg atatttcaaa tagagaagat gtagaggata
    22921 tggtagatta ttgtataaaa gaatttggag ggttggatgt acttgtaaac aatgctggtg
    22981 taagtcaaga caagttgttt acagatataa ctgatgaaga ttgggataat atgatgaata
    23041 ttaatttaaa aggaagcttt tattgttcac aagttgcttt aaaatacatg atatcagaga
    23101 aaaaaggaaa tataattaat atatcttcaa tctggggtat atcaggtgcc tcttgtgaag
    23161 tgcattattc tataacaaaa gcaggtataa taggcatgac aaaggcatta gctaaagaag
    23221 taggaccatc aaatattaga gtaaatagca tagctccagg tgtcattaat actgacatgt
    23281 tatctggata taatgaggaa gatatagacg tccttgtgga agaaacccct ctaatgaggc
    23341 taggaactcc agaagatata gcaaattgtg ctattttctt ggcatcagac aaatcaaatt
    23401 ttatcactgg tcaggtaatt agtccaaacg gaggatttgt tatttaaaaa aaatgtggta
    23461 ttatataaaa ataggaaaga ataacacgat atactactca taaacagtat gtgtttatat
    23521 aaaaattata aagggaggca tttgatgcta gaaggaaaaa ttataaaggg gattagtgga
    23581 ttttactatg tagatacaga taatggtatt tatgaatgta aggctagagg aatatttaga
    23641 aaacagaaaa taacacctct agttggtgac agagtcaaaa taagtattgt tgatgaagat
    23701 gaaaaaaaag gtatcttaga agaagtagac agtagagata cagagcttat aagacctcct
    23761 atagcaaatg tagataaggc attaatagta tttgcaataa aaaatcctaa accaaactta
    23821 tctcttttag atagatttat agttttggca gaaaaagaaa atttagaaac tgtaataatc
    23881 cttacaaaag cagatttaga tgataatgat acattagaaa ctgttaaaaa tatatatgaa
    23941 cttagtggct ataaagtcat tcctgtaagc aatattacta agttaaatat agataaagta
    24001 aaagaagaat taaaagaaaa tgtagtagtg tttgcaggac catctggagt tggaaaatca
    24061 agcttactaa atgaaataga tgaaaatttt aaacttcaaa caggagtggt aagtgataaa
    24121 ataaaaaggg gaaaacatac tactcgtcac gctgaacttt taaaattgga atttggtgga
    24181 atggttgcag atacaccagg ttttagttcc cttgcacttg aagatataga ggaagttgaa
    24241 cttaaagatt attttataga atttgataaa tttaatgatt gtaagtttgg ttcaaagtgt
    24301 attcatgaaa atgaacctaa ttgtgctata aaagaagctg ttgcaaatgg agaaatatct
    24361 aaagaaagat atgatagtta tatacagcta ctaaatgaaa taagacaaaa taacagtagg
    24421 aggtattaga aacatgatta aattagcacc atcaatatta tcagcagatt ttgcaaaatt
    24481 attagaggat gtaagaaaag tagaaagtgc aggatgtgaa tatcttcata tcgatgtaat
    24541 ggatggacac tttgtaccaa acattacatt aggaccactt gtagtcaaaa gtttaaagaa
    24601 agaaaatata aatatggtat ttgatgctca tcttatgata gaaaatcccg accagtatat
    24661 tgaagaattt gtaaaagcag gatgtgatat aataactgtt caccaggagg cttgtgttca
    24721 tctacataga actatacaaa atataaaatc acatggaata aaagctggtg tagtacttaa
    24781 tccagctaca ccagtagata cgattaaaca tgtactacct gatttagata tggtgttatt
    24841 aatgtctgtt aaccctggat ttggaggaca atcatttatt ccttgtgttt tagataagat
    24901 taaagagtta aaagctatta tagatagtca aggattaaac attgatatag aagttgatgg
    24961 tggtataagc cctaaaaacg tagccgaagt tgtacaagca ggagctaatg taatagtagc
    25021 aggttctgca atatttggaa gtgatgacat tcaagaaaca gttaatttat ttagaaagaa
    25081 tgcttctctt gaagaattag tttaaattta ataatgaaaa tctgtattgt tttaaatggc
    25141 gaaattgaag attacgaagt tacaagagat attataatta aggaatgtta tgactgcata
    25201 atatgtgcag atggaggggc aaatcatact tataaaatgg aaataatgcc agattatata
    25261 ttaggagatt tagattcagt agaagaagaa aagataaact tctataaaaa taaaggtgtt
    25321 aaatttgaaa aatttccatc taaaaaagat gagactgata cagagctttg tcttttttta
    25381 gctaagacac ttaaagtaaa tcatattgat ttttttggtg ctttaggggg aaggattgac
    25441 cacactcttg caaatataaa gctattatat tatttaaaag aagatggtat atattctagg
    25501 atactttctg ataaagaaga gatgtatata gttgaaaatg aggaaatttc attgtatgga
    25561 aacccagggg acactatttc tgtaatagct ataaatggtg atgctaaggg tgtaacactt
    25621 actggcttag aataccctct cgataattat tatatgaaat actctgtacc tattggaata
    25681 tcaaatgtta tgttgagtaa ttcttgtaag attaaagtag agcaaggctg tgtacttgta
    25741 gttagaaatt tataaaattt gattttgaca attgatttta tattgtagag cagatacaat
    25801 caagtaaaat atatttatga ttgtatctgc tttttagtat ataattataa atatataata
    25861 aactttgaca taagggggat tttttgtggt agatagtatt atttttgatt tagatggaac
    25921 attgtgggat tcaactgaag aagtatgcaa agtctggcag gatgttttaa gtaaacataa
    25981 ggaaatagaa ttaagtgtca caaaagattt atttagaagt cttatggggc tttcttttga
    26041 ggaaatagca aatcgtttat ttccaaattt aagtgaagaa gaaagaatga atttattgga
    26101 tgaatgttct ttgaaagaat gtgactattt atctgaaaat ggagctaaat tatttgatga
    26161 tatagaagat actcttaaat tactatctaa aaaatataaa ttatttatag taagtaattg
    26221 tcaagctgga tatatagaat cttttttaaa agcacataaa ctagaacagt attttataga
    26281 ttttgaatgt cctggtaata ctggtttgca taaaggtgaa aataataaac ttataattga
    26341 aagaaataaa ttaataaatc caatctatgt tggtgatacg caaggtgatg caaatagtgc
    26401 taagtttgta ggtatacctt ttatatatgc caaatatggg tttgggaatg tagatgtata
    26461 tgattatttt atagactcat ttaaagattt gttagaacat gatatattaa aataagatga
    26521 gtttaaaaat tatattaaaa taataaaaaa gttatggggg gatttaaaaa tggaattaca
    26581 agatactatt tttaaaagac aaagtgtaag aaagtttaaa aatcaagatg tattagatga
    26641 agatatttta aaaatgataa aagcagcagg tgcagcacca tcaggcaaga atattcaaaa
    26701 ctggcatttt gttgttataa aacgccgtga tttaatggaa aagatagcag atgtgataac
    26761 taaaaagcaa caagaaatac ttgtagaaat ggataaagta tcagtggata aggctaatag
    26821 atttagaaaa tttgttaaaa actttacact attttactta aaagctccag ttttagtatt
    26881 agtttttaca aaggtgtata acccatcagg atattatgaa ttagagctta tagatgcacc
    26941 taaggagact atagataaat tatttataag aaatccaggt atgcagagtc taggtgctgc
    27001 aattgaaaac tttacactat cagcaattga acttggatat ggttcttgtt ggttgacaag
    27061 tcaaaactat gcagcagatg agatagaagc tgttttagaa gcagaaactg gttttgaaaa
    27121 gggagagtat tttttaggtg caatgttagc tcttggagtt ccagaggaca atttgaagag
    27181 tccatctaaa aaaccagtag aagaaatatg tacatttata aaataaaagt caagggcatt
    27241 ttaaaatcga agtaaattaa aaacaacata aattaaaaat aaatatttaa aaaattaaaa
    27301 agtaggtttt attgatatta attttatatt tcaaatcatt gatttaat
//
LOCUS       DAEPXF010000036        27104 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain HB27
            SAMN13376389-rid12479423.denovo.038, whole genome shotgun sequence.
ACCESSION   DAEPXF010000036 DAEPXF010000000
VERSION     DAEPXF010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13376389
            Sequence Read Archive: SRR10518432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 27104)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27104)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001069624.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001069624.1
            Genome Coverage       :: 315x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/15/2021 01:54:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,165
            CDSs (total)                      :: 4,076
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 89
            rRNAs                             :: 10, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 10 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 19 of 69
            Pseudo Genes (incomplete)         :: 44 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 10 of 69
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27104
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13376389-rid12479423.denovo.038"
                     /strain="HB27"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="China: Hebei"
                     /collection_date="2017"
     gene            32..913
                     /locus_tag="KRL67_001358"
     CDS             32..913
                     /locus_tag="KRL67_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4300 family protein"
                     /protein_id="HBH1522253.1"
                     /translation="MKKRLIIMILSVVLVLSSILTIFAYSNIKYNNNKLIYSNMIDKK
                     TQNSVKEILKENKINEKDIDTFIKAVNNYNKLQVKILQNNINISKSGYSSINAKQVPY
                     NLEKLQDNWVKKFPDYMDVNCRITAFRLFKDFINSSKKFTGDSIDLNVDLDTIMNNKD
                     AKFSTKDVEKFINFFSAIPAKDTDDSIKIAEQIKNEWKKRKISFKNKKNISIVNGFLR
                     YPETKNVFIGHTGICIKTKNGILFLEKYGVTSPYQVTKFKNKKDVKNYMFNRLKMSEG
                     EIELPDPIIMENDKLMK"
     gene            complement(1226..2005)
                     /gene="hisJ"
                     /locus_tag="KRL67_001359"
     CDS             complement(1226..2005)
                     /gene="hisJ"
                     /locus_tag="KRL67_001359"
                     /EC_number="3.1.3.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphatase HisJ"
                     /protein_id="HBH1522254.1"
                     /translation="MKDGHIHSPYCPHGSKDDFEKYVQRAIDVGITEMTFTEHFPYAN
                     GFRDPAPENDSCMSIEDLPKYFNDVKKLKEKYEGKIKINVGSEVDFIEGYEEGIKANL
                     NKYGEELEDSLLSVHIIKINNNYYCVDYSVEEFQNLIDKLGSIEAVYNKYYETLIKAV
                     NSDLGIHKPKRIGHLNLVRKFNQVFPYDYKGNTILEDLIKLIKEKGYELDYNVSGNRK
                     EYCKEPYIDGYLLELVKKYDIPLVLGSDSHCAEEINKYDTI"
     gene            complement(2551..2901)
                     /locus_tag="KRL67_001360"
     CDS             complement(2551..2901)
                     /locus_tag="KRL67_001360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter"
                     /protein_id="HBH1522255.1"
                     /translation="MIFDKEIVRYHHGHHDHDHCHEHTHGDICHEHPHDHAHDHDHEH
                     SHEESSESKDEKTLKILLVHWINHNETHEEGFREWVEKARAIGKEETAKSIEKAIEYM
                     EEANKMLLEAKKHM"
     gene            complement(2917..3333)
                     /locus_tag="KRL67_001361"
     CDS             complement(2917..3333)
                     /locus_tag="KRL67_001361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3842 family protein"
                     /protein_id="HBH1522256.1"
                     /translation="MIIAVIDGMGGGIGAQIVSSLREELPTYVEIYALGTNSIATSSM
                     MKAHANKGATGENAIVVSAKKANIIVAPISVIIPNSMMGEVTCNISEAIADSEALKIL
                     LPIMPENVELVGLEGKPLALLVKDSVNLIKKEFNIK"
     gene            complement(3352..3543)
                     /locus_tag="KRL67_001362"
     CDS             complement(3352..3543)
                     /locus_tag="KRL67_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CooT family nickel-binding protein"
                     /protein_id="HBH1522257.1"
                     /translation="MCESSAFICKSNNELEKVMENVVNIDPCDGKIYLTDLLGEQKII
                     DGIIKEIRLMDHKIIIQEN"
     gene            complement(3694..4509)
                     /locus_tag="KRL67_001363"
     CDS             complement(3694..4509)
                     /locus_tag="KRL67_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase family protein"
                     /protein_id="HBH1522258.1"
                     /translation="MNQWRVIHNKSYEGAMNMAIDEAIFTAYKKGHNKPTLRFYTWEP
                     ACLSIGYFQKLEDEIDLDKCRCMNIDYTRRITGGRAVLHDKELTYSIIIGEDNPLIDK
                     SINMSYRYISEGLVKGLNLSGIKTDNLNRGERISRENLSAACFNAHASYEVTINNKKV
                     IGSAQSRKDGVLLQHGSIILDFDVEKLFKLIKTKTPELKERAMKFTAKKASGIENEIG
                     RRIDIDILQKNIVKGLAEQFNVEFVEGDLTDYEKQLAKELYEKYKNEEYNKKR"
     gene            complement(4556..5494)
                     /locus_tag="KRL67_001364"
     CDS             complement(4556..5494)
                     /locus_tag="KRL67_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HBH1522259.1"
                     /translation="MIRLSVGTAIELEILDKKSDIPPTTAYIMIGEKCINKCSFCSQS
                     IESSTRKDKLSRVIWPEFSKEEILDALKTYKGKNIKRICIQSMASEEAHNSVLDFINY
                     ISGKIDMPISLSAKLENDEQIKKFFSAGVDKIGIAIDAANKELYEKIKGNNYDEKLKF
                     ITEMSKSYPNKISTHIIVGIGESHEDIYNLYTYLKENDITISLFAFTPVRGTKMEKIN
                     QPSIESYRRVQLMSYMINKGYPKECFKFKNGYLDSIKLDNDIVKDINKGYPFEIRGCK
                     DCNRPYYNERPGSTIYNYSRPLNQSEIDLAIREINL"
     gene            complement(5491..6363)
                     /locus_tag="KRL67_001365"
     CDS             complement(5491..6363)
                     /locus_tag="KRL67_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HBH1522260.1"
                     /translation="MNKNLSEKMKIAFDIKLKNFGNKIEFAYPNQTLALSTTSNQCSL
                     KCAHCNGHYLNNMVPIEEYEEKVQSRNITSFLLSGGCSYEGDVPINTHINTIKNLKEQ
                     GYRLNAHLGLMDKESIVELCKYLDIVSFDLVFDDETIREVYKMKKSKEDYIEVYNTIR
                     EHTEVAPHICIGLKGGQIKGEYEIVKYLQKNPPNKLTFIVLIPTKGTEYENVEPPELE
                     GVADILCEARINLPDTEINLGCMRPRGVYRKELDQLSIMCGVNRIVLPSRSAKNKAIE
                     MNMTITECKECCVL"
     gene            complement(6388..8316)
                     /locus_tag="KRL67_001366"
     CDS             complement(6388..8316)
                     /locus_tag="KRL67_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4445 domain-containing protein"
                     /protein_id="HBH1522261.1"
                     /translation="MIKVSFTPNNKEVYCNEGDILLEVARNADIFIDAPCNGNVSCGK
                     CKVKLLNGKVDTEKTRHITDDEWEQGYILACCTKVISDIEIEVPSKVSSSMHGMKIEG
                     SNKKEDREIFERAKKIIEEHNLQFKTNIKKKYIEMEEPNLDDNISDVDRLERYVRNNL
                     GYNEIDFRLDILRKMPTVFRKSDFKVTITYVQKQKKLTIINIEQGNKENSLYGVAIDI
                     GTTSVVVCLVDLYSKEVVDKASSGNAQIKYGADVINRIIYSTKKNGLETLHKAIVEET
                     INPLLKTIYERNGIDKEDVVTLVAAGNTTMTSLFLGVYTDFLRQEPYIPPFLKSPKLM
                     GENVGLFVNDSAYVYLAPSVASYVGGDITAGVLSAGIWSSEENVLFIDLGTNGEIVFG
                     NKDYMMSCACSAGPAFEGGGISCGMRASAGAIEKVIIDKETLEPTLKIIDECAPVGIC
                     GSGIIDLICQMITKGVIDRRGKIYRDLDNKRVRFNEHEIGEYVLAFKEEFDLENDIVV
                     NEVDIDNFIRAKGAIYSGAYTLVDSLGMDFSILDRVYIAGGIGNNLDIENSIIIGLLP
                     DIDREKFTYIGNSSLVGSYLALISKDAKNKLEEIGNQITYVELSVYPSYMDEFISACF
                     LPHTNIEQFPTAKKLLEE"
     gene            complement(8428..8805)
                     /gene="gcvH"
                     /locus_tag="KRL67_001367"
     CDS             complement(8428..8805)
                     /gene="gcvH"
                     /locus_tag="KRL67_001367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HBH1522262.1"
                     /translation="MKLLPELKYSKDHEWVKVIDGDVVYIGITDYAQDQLGEILFVET
                     PEVEDTVTKGVDFGVVESSKVASDLISPVNGEVLEVNEKLEDEPECINEDPYENWILK
                     VKLADVAELDTLLSDKEYEAGLE"
     gene            complement(8892..11018)
                     /gene="cdhC"
                     /locus_tag="KRL67_001368"
     CDS             complement(8892..11018)
                     /gene="cdhC"
                     /locus_tag="KRL67_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CO dehydrogenase/CO-methylating acetyl-CoA
                     synthase complex subunit beta"
                     /protein_id="HBH1522263.1"
                     /translation="MNLYNIIFTGSEQALGAAQAMLAEAIEKNGKEHKVAFPDTAYSL
                     PCIYAATGQKMNTLGDLEGALEVVKSLINRTHLLEHAFNAGLATALAAEVIEALKYST
                     MDAPYSEPCAGHITDPIIRSLGVPLVTGDIPGVAVVLGECPDAESAAKVIKDYQSKGL
                     LTFLVGKVIDQAIEAGVKMGLELRVIPLGYDVTSVIHVVSVAVRAALIFGGLTPGDLN
                     GLLEYTANRVPAFVNAFGPLSELVVSAGAGAIALGFPVVTDQTVLEVPMNLLTQKDYD
                     KIVATSLEARGIKIKVTEIPIPVSFAAAFEGERIRKSDMFAEFGGNRTEAWELVVKKE
                     ATEVEDHKIEIIGPNIDEVDADGVLRLPLAVIVKIAGKNMQEDFEPVLERRFHYFLNY
                     IEGVMHVGQRDMAWVRISKDAFDKGFRLEHIGEVLYAKMLDEFESVVDKCEITIITDA
                     EKVSELKGEAIAKYNARDERLASLVDESVDTFYSCNLCQSFAPAHVCVVTPERLGLCG
                     AVSWLDAKATKELDPTGPCQPIEKGECLDDRTGVWNSVNETVNQISQGAVESVTLYSI
                     LEDPMTSCGCFECICGIMPEANGFVVVNREFASVTPVGMTFGELASMTGGGVQTPGFM
                     GHGRHFISSKKFAYAEGGPERIVWMPKELKDYVADKLNATVKEMTGIENFCDMVCDET
                     IADDSEGVLAFLEEKGHPALAMESVM"
     gene            complement(11075..11881)
                     /locus_tag="KRL67_001369"
     CDS             complement(11075..11881)
                     /locus_tag="KRL67_001369"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HBH1522264.1"
                     /translation="MEKFMIIGERIHCISPSIRKALAERDPAPILKRAKEQLEAGAHY
                     IDFNIGPAERDGEEIMTWGIKLLQSEFNNVPIALDTANKKAIEAGLKVYDRTNAKPII
                     NSADAGSRFDLIDIAAEYEAMVIGLCAKEGIPRDNDERMAYCQEILEKGLMLGMEPTD
                     ILFDPLCLVIKGMQEKQVEVLEAIKMMTEMGLLTTGGLSNVSNGCPKHVRPVLDSAFL
                     AMAMANGFSSAIMNPCDPELMKTVKSCDIINGASLYADSFLELNEGGFAF"
     gene            complement(11931..13298)
                     /locus_tag="KRL67_001370"
     CDS             complement(11931..13298)
                     /locus_tag="KRL67_001370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA decarbonylase/synthase complex
                     subunit gamma"
                     /protein_id="HBH1522265.1"
                     /translation="MALKALDIFKLTPKKNCKDCGFPTCMAFSMKVASGAVEVGKCPH
                     MSDDAIAKLSEATAPLMKALKVGAGASEYELGGETVLFRHEKTLVSRNRYAVSFCTCM
                     SDEAVDSKIANMKKVDYVRIGEQMKVEMAVLEYCGDKDAYLKLIDKIKGSELEVAYIL
                     ACDDAQVVKEAVEVLKDARPMVYGATKENYKDMIEVVKGASLPLGVKAGSLEELYETV
                     ELIQAAGYKELVLDVTGENIKDTYTNAIQVRRIALKEQDRTFGYPSIVFANRLSNSNP
                     MMEVALSSIFTIKYGSIIVIDDISYAKALPLFALRQNIYTDPQRPMRVEPKIYPINNP
                     DENSPVLVTVDFALTYFIVAGDIERSKVPVWLVIPDAGGYSVLTSWAAGKFGGNSISA
                     FIKESKVEEVTNCKDLIIPGKVAVLKGDIEDNLPGWNVVIGPEESMELPKFLKGYQEK
                     ACQTN"
     gene            complement(13329..14273)
                     /locus_tag="KRL67_001371"
     CDS             complement(13329..14273)
                     /locus_tag="KRL67_001371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA decarbonylase/synthase complex
                     subunit delta"
                     /protein_id="HBH1522266.1"
                     /translation="MAFKMSTQKYSGKISEVEVGIGEKAIKLGGENVLPFYSFDGEVG
                     NSPKIGIQISDVYPESWTDSYKELYKDVANCPVEWAKYVEANTQADFICLKFDGSDPN
                     GLDKSVDECADVAKAVIEAIKLPLVVAGSGNHEKDGKLFEKLAQTLDGHNCLFMSAVE
                     DNYKGVGASAGMAYAHKVGAESSVDINLAKQLNVLLTQLGVKGENIVMNVGCSAVGYG
                     YEYVASTMDRIRLAAFGQNDKTLQMPIITPVAFEVGHVKEAIAPIEDEPDWGCPEERT
                     IAMEVSTAASVLVGGSNAVILRHPKSIETIKELVNALA"
     gene            complement(14293..15063)
                     /locus_tag="KRL67_001372"
     CDS             complement(14293..15063)
                     /locus_tag="KRL67_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBH1522267.1"
                     /translation="MGYNIAVAGKGGTGKTSLTGLLIDYLVKDKKGPVLVVDADANAN
                     INEVLGIEVEATIGEIREEVNQREKLGNAFPGGMTKAQYLQFRLNSIIEEGEGYDLLV
                     MGRSEGEGCYCFVNGILREQVNKISGHYKYLVMDNEAGMEHLSRKVTRHVDTLLLVSD
                     CSRRSIQAVARIRDLAEELKLSVGRILLIVNKVPNGVMNDGVKEEIEKHNLELIGVVP
                     MDELIYEYDSTGIPLVNLPEDSKSKVAMKEIFAKLELK"
     gene            complement(15119..16504)
                     /gene="lpdA"
                     /locus_tag="KRL67_001373"
     CDS             complement(15119..16504)
                     /gene="lpdA"
                     /locus_tag="KRL67_001373"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HBH1522268.1"
                     /translation="MKIVVVGGGPGGYVAAIKASMLGADVTVVEKRRVGGTCLNAGCI
                     PTKALLASSGVLNTVKEAKDFGIEIDGTVKPNFTAIMERKNKVVNQLISGIEFLFEKR
                     GVNLVNGFGKLIDKNTIEVTKDDGTVETIKADKIILANGSVPVVPRMFPYDGKVVITS
                     DEVLGLEEIPESMLIVGGGVIGCEIGQFFRALGTEVTIVEMVDQILLNEDKDVAKQLL
                     RQFKKDKIKVITGIGVQTCEVVDGKAVATLSNGKVIEAQYALVCVGRRPNLDNSGVED
                     IGIEMERGKVVVNEHLETNVEGIYAIGDIIDTPFLAHVASKEGIVAVENALGKTKVVD
                     YRAIPRCVYTEPEVAGVGKTEKQLEAEGVEYNVGQFDFRGLGKAQAIGHFQGFVKVIA
                     DKETDKIIGAAVVGPHATDLLTELSLAVHLGLTVEQVGDAIHPHPSLSEGLMEALHDV
                     HGECVHSVPKL"
     gene            complement(16548..17429)
                     /locus_tag="KRL67_001374"
     CDS             complement(16548..17429)
                     /locus_tag="KRL67_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HBH1522269.1"
                     /translation="MSLLRETLESGKFAVTTEMAPPKGTDLSHLIECAKPLVGRVHAA
                     NVTDFQSAVMRATSLATCKLLKDAGLEPVIQITGRDRNRIAIQGEMLSAGVFGINNLL
                     ALTGDHTSVGDHPQAKGVFDLDSVGILQTAETLMAGTDMAGNKLKGSPDFYLGASVTP
                     EYSPIEVQLLKMQKKIKAGAKFFQTQALYDINTMRKFRELTRDMDCKVLAGIVPLKSP
                     GMAKFMTANVPGIFVPDEQIERLRAAGKENWVSEGIKMAGELIKQLKEEDLCDGVHIM
                     AIGAEENIPAILDAAGL"
     gene            complement(17465..18139)
                     /locus_tag="KRL67_001375"
     CDS             complement(17465..18139)
                     /locus_tag="KRL67_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase C-terminal
                     domain-containing protein"
                     /protein_id="HBH1522270.1"
                     /translation="MIISENKPLEEVLGYLKDFDKLVLVGCNQCAATCKSGGEEEVLK
                     MKETLEGEGKKILGYVMLDPACNLLKSKKDLKALKEETKEADAVLSLACGDGTQTIVK
                     NLKNKPVYPANNTLFIGEVQRVGEYEEACKACGDCELGWTGGICPVTMCAKGLMNGAC
                     GGAKNGKCEVNSENDCAWIKIYERLEAIGQLDNLAEIRPPKDYSKQNNPRSLSAKKKK
                     EAAANS"
     gene            complement(18176..19048)
                     /locus_tag="KRL67_001376"
     CDS             complement(18176..19048)
                     /locus_tag="KRL67_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 5,10-methylene-tetrahydrofolate
                     dehydrogenase/5,10-methylene-tetrahydrofolate
                     cyclohydrolase"
                     /protein_id="HBH1522271.1"
                     /translation="MEGMSTKGQIIKGKPVADKISEELIKEVDLLVKEGINPKLTIVR
                     VGARSDDLSYERGALKRCQNIGITTEVLELAEDITQEEYIDVLKRVNDDKNVNGILCF
                     RPLPKHLNEEVIKYVIAPEKDVDCFSPINSAKVMEGDKSGFPPCTPTAVVEILKHYNV
                     DLKGSKVTVLGRSMVVGKPVSMLLLSEHATVTICHSKTKNLSGVAAEADVLIAAIGRA
                     KMVDESFVKDGAVVIDVGINVDEEGNLCGDVDTNAVLDKVSMITPVPAGVGSVTTSIL
                     AKHVVKACKLQNNK"
     gene            complement(19140..19772)
                     /locus_tag="KRL67_001377"
     CDS             complement(19140..19772)
                     /locus_tag="KRL67_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclodeaminase/cyclohydrolase family protein"
                     /protein_id="HBH1522272.1"
                     /translation="MKIADKTCVDFVEVLSSKEAVPGGGGAAALVGAIGMALGSMVCN
                     LTIGKKKYAEYEESVKEILSKAGKLEKDLLKMIDDDAECFLPLSKAYGLPKETEEEKR
                     IKAETMEKALKVACEVPLNIVRVCYEAIKLHEDLVDKGSRLAISDVGVGVQCLRAAIL
                     GGQLNVVININSIQDKEYVNKVKTEVDKLVEDGVKICDEVYAKVEKALGK"
     gene            complement(19877..21553)
                     /locus_tag="KRL67_001378"
     CDS             complement(19877..21553)
                     /locus_tag="KRL67_001378"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014355320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HBH1522273.1"
                     /translation="MGFKSDIEIAQEAKPQDIREVAKKLGLGEDDVELYGKYKAKVDY
                     NLLKRETGKKAKLILTTAINPTPAGEGKTTTTIGVADAFAKLDKNVLVALREPSLGPV
                     FGVKGGAAGGGYAQVVPMEDINLHFTGDFHAIGAANNLLAAMLDNHIHQGNALRIDPK
                     KITWRRCVDMNDRQLRNIVDGMGKKGDGAVRQDGFDITVASEIMAAFCLASDISDLKE
                     RLGNIIVGYSYEGEPVTARQLKANGAMAALLKDALKPNLVQTLEGTPSFVHGGPFANI
                     AHGCNSVIATRMAMHFADYVITEAGFGADLGAEKFLDIKCRMANLKPDAVIIVATVRA
                     LKYNGGVAKADLNNENLEALEKGLPNLLKHVENITQVYGLPAVVAINRFPLDTEAELK
                     LVEDKCKELGVNVALSEVWAKGGEGGIAVAKEVLRLLDEEENNFRFCYEDDLSIKDKI
                     NAIATKIYGADGVDYTPEADKEIANLERLGFTKVPVCMAKTQYSLTDDQTKLGRPTGF
                     RITVRQATISAGAGFIVALTGEIMKMPGLPKVPAAEKIDVDENGVIAGLF"
     gene            complement(21802..22581)
                     /locus_tag="KRL67_001379"
     CDS             complement(21802..22581)
                     /locus_tag="KRL67_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBH1522274.1"
                     /translation="MKIAITGKGGVGKTTFSSMLSRMFAEDGYRVVAVDADPDANLAL
                     ALGFPKEVYESIVPISEMKKLVSDRTAASVGSFGKMFKMNPKVDDIPENFCKEYNGVR
                     LLTLGTVDSGGTGCVCPEHVLLKRLCSHLILQNKDVVVMDMEAGIEHLGRGTAQGVDA
                     FIVVVEPGERSLQTYRKVKKLGHDIGVNKVFVVGNKIRNKEDEEFIIQNLEDGESLGF
                     IYYNQDVIDSDRANQSPYDSSETTKEQIKAIKDKLMSLKDK"
     gene            complement(22881..24800)
                     /gene="cooS"
                     /locus_tag="KRL67_001380"
     CDS             complement(22881..24800)
                     /gene="cooS"
                     /locus_tag="KRL67_001380"
                     /EC_number="1.2.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic carbon-monoxide dehydrogenase
                     catalytic subunit"
                     /protein_id="HBH1522275.1"
                     /translation="MDEKMLTIDLNSQKMIAKAREEGVETMYDRKEGFKAQCGFGLQG
                     VCCRICGMGPCRISPKTPRGLCGADEHTIVGRNFARMVAGGTAAHSDHARDIAHTLAL
                     ADPNGNYKIRDEAKLITLAKEWDVETEGRDIYDVAHEVAEIALMEFGKPFGTLRFIKN
                     APEPRQKIWKEYAIEPRAIDREIATIMHSTHIGCTGDIDSLIHMSLRTSMADGWGGSM
                     IGTRLSDILFGTPVPRRTEANLAVLEENKVNIILHGHEPALSEMIVLASEEPDLVALA
                     KEVGADGINLAGMCCTGNEITMRHGVKIAGDFHQQELAIITGAVEAVIVDVQCIFPAL
                     ARVADCYHTKFVTTSPKAKITGSTYIEFREEQALDDAKAIVKEAILNFKNRDKSKVLI
                     PELKSGATVGYSVEAVVNQLDRVVNSHIDPAGTVKPLTDCLKAGVLRGAAGVVGCNNA
                     KGVSNEAHVTIMKELIKNDIIVVTTGCGASAAAKFGLMESDAAEKYAGKGLATVCKLV
                     GIPPVLHMGSCVDISRILDLVGAAANYLDMDMCDLPVVGIAPEWMSEKAVAIGCYVVA
                     SGIDTYLGIMPPIAGSSRAVDILTNELKDKVGATFTVNTNPKELAATIIEDIEKKRVH
                     FEALVEEKMAEKAEA"
     gene            complement(25407..26318)
                     /locus_tag="KRL67_001381"
     CDS             complement(25407..26318)
                     /locus_tag="KRL67_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional enoyl-CoA hydratase/phosphate
                     acetyltransferase"
                     /protein_id="HBH1522276.1"
                     /translation="MIKKLDDILDQLRGGEKIVLSVAAAHDKEVLMAIKDAVERNIIT
                     PILVGNEGKIREISKEIGFDLSGIKIVDKDDIKECAEIAVKLVSSKEADFVMKGLLDT
                     SVILKEVLNKDYGLRTDSLLSHVMVYELEKYHKLLITTDGGMNIAPDYEQKAKILKNS
                     IKAAKALGMETVKVACLAAKEKVNPKMQATVDADMLAKACKEGEFGENVIVEGPLAFD
                     LAVSKEASEIKGFKSEVSGDVDIILVPTIEVGNGIGKAFTYMANSKSAGIIMGAKAPI
                     VLVSRADSHESKLYSIAYGAIVAKNMK"
     gene            complement(26434..27012)
                     /locus_tag="KRL67_001382"
     CDS             complement(26434..27012)
                     /locus_tag="KRL67_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HBH1522277.1"
                     /translation="MNINEFLTHVNQRKPVFSKSEIHEFMVRLSNEAMKITSKLNSSY
                     HEPEEVRSLFSELIGKKVDESFFMFPPFYTDCGKNITIGKNVFINSSCHFQDQGGIEI
                     GDNTQIGHNVVLATLNHGVAPEKRGTTYPSPITIGKNVWIGANVTVVPGVTIGDNAII
                     AAGAVVTKNVAENTIVGGVPAKLLRVIDEIPE"
BASE COUNT         8099 a         5489 c         3087 g        10429 t
ORIGIN      
        1 gacacaataa atttaaaaag gagaactaaa tatgaaaaaa agattaataa taatgatatt
       61 gtctgttgtc ttagtactat caagtatact tacgatattt gcatattcaa atattaaata
      121 taataataat aaattgatat attctaatat gatagataaa aaaactcaaa atagtgtaaa
      181 ggaaatccta aaagaaaata aaattaatga aaaagacata gatacattta ttaaagcagt
      241 caacaattat aataaattgc aagttaaaat acttcaaaat aatataaata tatctaaatc
      301 tggatattct tcaatcaatg caaaacaagt tccatataac ctagaaaaat tgcaagataa
      361 ttgggtgaaa aaattccctg attatatgga tgtaaattgt agaataactg cttttagact
      421 ttttaaagat tttataaatt caagtaagaa atttactgga gactctattg atttgaatgt
      481 cgatttggac actataatga ataataaaga tgctaaattt agcaccaaag atgtagaaaa
      541 atttatcaac ttcttttctg caataccagc taaagataca gatgattcaa tcaaaattgc
      601 agaacaaata aaaaatgaat ggaaaaaaag aaaaatatct tttaaaaata agaaaaatat
      661 atctattgta aatggatttc ttcgttatcc agaaactaaa aacgtattta taggtcatac
      721 aggtatatgt attaaaacta aaaatggcat tctattttta gaaaaatacg gtgttacatc
      781 accttatcaa gttactaaat ttaaaaataa aaaagatgta aaaaattata tgtttaatag
      841 attgaagatg tcagaaggtg aaattgaatt gcctgaccct ataataatgg aaaacgataa
      901 acttatgaaa taataacttt aaacgcaaaa tagtatttat aattactaag tcaatataat
      961 agagaaggtc tatagatttg tgtaaataca agcttatcta caatcttata taagaatgtt
     1021 tatataaata agatagatta attctaagtc ctattattct attgaatttt attattattt
     1081 tacttagatt ctattgttat tttacttaga acttattatt attttattta agttctatta
     1141 ctattttcaa tcatataaat ttacaaaaga ctgtacataa atttatttta tacaaactta
     1201 tctacagtct tttgtgtgaa tatatctaaa tagtatcgta tttatttatt tcctctgcac
     1261 agtgagaatc agaacctaaa actaatggaa tatcgtattt ttttaccaac tctaataagt
     1321 atccatctat ataaggctct ttgcagtact cttttctatt tccagaaaca ttataatcaa
     1381 gttcataacc tttttcttta attaacttta ttaagtcttc aagaattgta tttcctttat
     1441 aatcgtatgg aaatacctga ttaaactttc taactaaatt taaatgacct attctttttg
     1501 gtttatgtat acccaaatct gaatttacag cttttatcaa tgtttcataa tacttattat
     1561 aaacagcttc aatacttcct aacttgtcga ttaaattttg aaactcctct acactgtaat
     1621 ctacacaata ataattatta ttaatcttaa ttatatgtac tgataaaaga gaatcctcta
     1681 attcttctcc atatttattt aaattcgcct ttattccttc ttcgtatcct tctataaaat
     1741 ccacttctga cccaacattt atcttgattt taccctcata cttttctttc aacttcttga
     1801 catcattaaa atactttggc aaatcttcaa tactcataca actatcattt tcaggagcag
     1861 ggtctctaaa tccatttgca tacggaaaat gttccgtaaa tgtcatttct gttattccaa
     1921 catcaattgc tctttgtaca tatttttcaa aatcatcctt actcccatga ggacaataag
     1981 gtgaatgtat atgaccatct ttcataaata attcactcct tttttaaaga aaaagtctaa
     2041 tataaataca taaatctttt aacttcaaaa caatattttt aattatacta attcttatta
     2101 tatctaatat tagagtaatt ttaaactgcc ttttaagtat cttatcatta gtactatttt
     2161 caacttaaca ttaatattat tttttaactt aacattaata ctatctttaa atagtaatac
     2221 tcaatatata agttacttaa catcaaataa ctcaaattgc ttgatgaggc ttttgcaata
     2281 tttaaagaat atataattat ttaacattaa ataatccaat cttgtaataa aatttactca
     2341 tcaaagtata ttacagaaaa tgcctcaaaa taaacttttt agttcatgag actctctttt
     2401 tgcatgaaat cagatgtatt tttatcattt caacaataaa agggaggctt atatccattt
     2461 tgagacagcc cattagataa aatttttgat aattaaacat cacaaaacat aaaaatcgct
     2521 tatatcaaac gtataagcga ttaattatat ttacatatgt tttttagcct ctaacaacat
     2581 tttattagct tcttccatat actctatagc cttttcaata ctctttgctg tttcttcttt
     2641 tccaatagct ctagcctttt caacccactc tctaaatcct tcttcatgag tttcattgtg
     2701 atttatccaa tgtactaaaa gtatttttaa agttttttca tccttagact ctgatgattc
     2761 ctcatgacta tgttcatggt cgtggtcgtg agcatggtca tgtggatgtt catggcatat
     2821 atctccatga gtatgttcgt ggcagtggtc atggtcatga tgtccatgat gatatcttac
     2881 aatctcttta tcaaaaatca ttagttattc cccctgttat tttatattaa actctttttt
     2941 tataaggttt acagaatctt ttacaagtag tgctaatggt ttaccttcta atccaactaa
     3001 ctccacattt tctggcataa taggtaaaag tatcttaagt gcctcactgt ccgcaatagc
     3061 ttcacttata ttacaagtaa cttctcccat catagaatta ggtattatta cagaaatagg
     3121 tgcaactatt atatttgctt ttttagctga tactactata gcattttccc ctgtagctcc
     3181 tttattagca tgagccttca tcatagaact agttgctata gaattagtcc ctagtgcata
     3241 aatttctaca tatgtaggta actcttctct caaagaagaa actatctggg ctccaatacc
     3301 tccacccatt ccatctatca ctgctattat catatgaaat tcccctatta attaattttc
     3361 ttgtattatt atcttatggt ccatcaatcg gatttcttta attatcccgt ctattatctt
     3421 ttgctctcct aataaatctg ttaaataaat ctttccatca caagggtcta tattaaccac
     3481 attttccata accttttcta actcattatt acttttacaa atgaaagctg atgattcaca
     3541 catataaatc tcctcctaat atgattttta ttaaaaatca aaaaaagatt tccttaggta
     3601 tttcaataca ccaaagaaac cttcatgatt tcagtttttg tatattatta taccttaagt
     3661 atatcacttt aatttatata aagtcaatta aagttaacgc tttttattat actcttcatt
     3721 cttgtatttt tcatatagtt ctttagctaa ttgcttttca taatcagtca aatcgccctc
     3781 cacaaactca acattaaatt gttcagctaa gccctttact atgtttttct gtagaatatc
     3841 tatgtctatt cttctgccaa tttcattttc tattccactt gcttttttag ctgtaaattt
     3901 catagccctt tctttcagtt ctggtgtctt agtttttatt aatttaaaca atttttctac
     3961 atcaaaatcc agtattattg aaccatgttg taataaaaca ccatcttttc tgctttgagc
     4021 acttccaatt accttcttat tattaatagt aacttcatat gatgcatgtg cattaaaaca
     4081 tgcagctgat aaattttccc tacttattct ctcgcctcta tttaaattat ctgtttttat
     4141 accacttaga ttcaatcctt taactagacc ttcacttata tatctataag acatgttgat
     4201 gctcttatcg attaaaggat tatcttctcc tataattata ctataagtta gctccttatc
     4261 atgtaataca gctcttccac ctgtaattct tctagtatag tctatattca tacatctaca
     4321 cttgtccaaa tctatctcat cttccaattt ctgaaaatat cctatactca aacaagctgg
     4381 ttcccatgta taaaatctaa gagttggctt attatgtccc ttcttatatg cagtaaatat
     4441 agcttcatca attgccatat tcattgctcc ttcatatgat ttattatgta taactctcca
     4501 ctgattcatg attattacgc ccccattttt agtataaata tatcctagaa atcctctata
     4561 gatttatttc tcttattgct aaatctatct cactttgatt taaaggtctt gaatagttat
     4621 atatagtact tcctggtctt tcattatagt atggtctatt acaatcctta caacctctta
     4681 tttcaaaagg atagcctttg tttatgtctt ttacaatatc attgtcaagc tttatactat
     4741 ctaagtatcc atttttaaac ttaaaacact cttttggata acctttattt atcatataag
     4801 acattaactg tacccttcta taactttcta tgcttggttg atttatcttt tccattttag
     4861 ttcctctaac tggagtaaat gcaaataaac taatcgttat atcattttcc tttaaataag
     4921 tatataaatt gtatatatct tcatgacttt cacctatacc aactattata tgtgtactta
     4981 ttttattggg atatgacttt gacatttcag ttataaattt taacttttca tcatagttat
     5041 ttccttttat tttttcatat agttctttat ttgctgcatc tattgctatt cctattttat
     5101 ctactcctgc tgaaaaaaac ttctttatct gttcatcatt ttctaatttt gcagatagtg
     5161 aaattggcat gtctatctta ccacttatat agtttataaa atctaaaaca gagttatgtg
     5221 cttcttcact tgccattgat tgaatacata ttctctttat attttttcct ttataagttt
     5281 ttaaagcatc taatatttct tcttttgaaa actctggcca aattactctt gataatttat
     5341 cttttctagt actactttct attgattgag aacaaaaact gcatttattt atgcattttt
     5401 ctccaatcat tatatatgct gtggtaggag gtatatcact ttttttgtct aatatttcaa
     5461 gttctattgc tgtcccaact gatagtctaa tcataaaacg caacactcct tacactcagt
     5521 tattgtcata ttcatttcta tagccttatt ctttgcagac cttgatggta atactattct
     5581 attgactcca cacattatag ataactggtc tagctctttt ctatacactc ctcttggcct
     5641 catacaccct agattaattt cagtatctgg taaattaatt ctagcttcac ataaaatatc
     5701 tgctacccct tctaactcag gaggttctac attttcatac tcagtccctt ttgtgggaat
     5761 aagtactata aatgtaagct tatttggagg atttttctgt aggtatttta caatttcata
     5821 ttcacccttt atttgtcctc cttttaaacc aatacatata tgtggtgcta cttctgtatg
     5881 ttcccgtatt gtattataaa cttcaatata atcttcttta ctctttttca tcttataaac
     5941 ttctcttatt gtctcatcat caaacactaa atcaaaagaa actatatcta aatacttaca
     6001 caactctaca atgctctctt tatccataag acctaaatga gcattcaatc tatatccttg
     6061 ctcttttaag tttttaatag tattaatatg agtatttatc gggacatctc cttcataact
     6121 acaaccgcca ctcaatagaa agctagttat attccttgat tgtacttttt cctcatattc
     6181 ttctattggc accatattgt ttaagtagtg accgttgcaa tgggcacatt tcaaactaca
     6241 ttgattgctt gtagtactaa gagctagtgt ctggttagga taagcaaact ctattttatt
     6301 tccaaaattc tttaatttaa tatcaaatgc tattttcatt ttttcagata gattcttatt
     6361 catagtttat tccttcttaa gtacatttta ctcttctagt aatttctttg ctgttgggaa
     6421 ctgttctata ttcgtatgtg gtaagaaaca tgcagaaata aattcatcca tataacttgg
     6481 atatacacta agttcaacat aagttatttg attaccgatt tcttcaagtt tattttttgc
     6541 atctttactt atgagagcta agtaagaacc aacaagtgaa ctattcccta tataagtaaa
     6601 tttttctcta tcaatatcag gaagtaaccc tataattatt gagttttcta tgtctaggtt
     6661 gtttcctata ccacctgcta tataaactct atctaatata ctaaaatcca tacctaaact
     6721 atctacaaga gtgtatgcac ctgaatatat agctccttta gctctgatga agttatctat
     6781 atcaacttca ttgactacta tatcattttc taaatcaaat tcttctttaa atgctaatac
     6841 atactctcct atctcatgct cattaaatct aactctctta ttatctaaat ccctgtatat
     6901 tttacctctt ctatctatta cacctttagt aatcatttga cagattaaat caattatacc
     6961 agaaccacat ataccaactg gtgcacattc atctataatc tttagtgttg gctctaaagt
     7021 ttctttatca ataattactt tctctattgc tcctgctgat gctctcattc cacagcttat
     7081 ccctccacct tcaaaagcag gtcctgctga acatgcacag ctcatcatat aatctttatt
     7141 accaaataca atctctccat tagttcctaa atctataaat aaaacatttt cttcacttga
     7201 ccatatccct gctgataata cacctgctgt tatatctcct cctacataac ttgcaacaga
     7261 tggagctaag tacacatatg cactgtcatt tacgaaaaga ccaacatttt cacccataag
     7321 ttttggagat ttcaaaaatg gtggtatgta aggttcttgt cttaagaagt ctgtatacac
     7381 acccaagaat aaacttgtca tagttgtatt cccagcagcc actaatgtaa ctacatcttc
     7441 cttatctatt ccatttcttt catatattgt ttttaatagt ggatttatag tttcctcaac
     7501 tatagcttta tgaagtgtct ctaatccatt tttcttagtt gaatatataa ttctatttat
     7561 aacatctgct ccatatttta tctgtgcatt tccagaagat gctttatcta ctacctcttt
     7621 tgagtatagg tctactaaac aaacaactac tgaagttgta ccaatatcta ttgcaactcc
     7681 atataatgaa ttttctttat ttccttgttc tatatttatg attgtaagtt tcttttgttt
     7741 ttgaacatat gttattgtaa ccttaaaatc actctttcta aatacggttg gcatctttct
     7801 aagtatatct aatctaaaat ctatttcatt ataccctagg ttatttctaa catatctttc
     7861 caatctatct acatcactta tattatcatc taaatttggt tcttccattt ctatgtattt
     7921 cttttttata tttgttttaa attgaagatt gtgttcttca attatttttt tagctctttc
     7981 aaaaatttcc ctatcctctt ttttattact accttctatc ttcataccat gcatagaaga
     8041 tgatacttta gaaggaactt caatttctat atctgatatc acctttgtac aacaagctag
     8101 tatataacct tgctcccact catcatctgt tatatgtctt gttttttctg tatcaacttt
     8161 tccattcaat aattttactt tacacttccc acaagacacg tttccattac aaggagcatc
     8221 tatgaatata tctgcatttc ttgcaacctc tagtaatata tccccctcat tacaataaac
     8281 ctctttattg tttggtgtaa aacttacttt tatcatgtta tccctcccaa tttgttctat
     8341 atatgataaa aggaaagtat agctaaattt gcgtgcactc aaattttact atactttccc
     8401 caattataat aatatcttaa tctttaatta ttctaatcca gcctcgtatt ctttatcact
     8461 taataacgta tcaagttcag ccacatcagc tagttttact tttaatatcc agttttcata
     8521 tgggtcttca tttatacatt ctggctcatc ttctaatttt tcattaactt ccaatacctc
     8581 accattaaca ggagatatta aatcagaagc tactttagaa gactcaacta ctccaaaatc
     8641 tactccctta gttacagtat cttctacttc tggtgtttca acaaacaata tttctcctaa
     8701 ttgatcttga gcatagtctg ttatacctat atacacaaca tctccatcaa ttacttttac
     8761 ccactcatga tctttagagt attttaattc tggtaataat ttcatttcga tagaccccca
     8821 atattatatt attgattaaa ttacttaaat catattaata ttatatcata ttattttaaa
     8881 ttttccatca attacattac actttccata gctaatgctg gatgaccttt ttcttctaag
     8941 aaagctaata ctccttcaga atcgtcagct atagtttcat cacaaaccat atcacagaag
     9001 ttttctattc cagtcatttc tttaactgta gcatttaatt tatcagctac ataatctttt
     9061 aattcttttg gcatccaaac tattctttct ggtccacctt ctgcataagc gaatttttta
     9121 gaagatatga aatgtcttcc gtgtcccata aatcctggag tttgaactcc tccacctgtc
     9181 atagaagcaa gttctccgaa agtcatacca actggagtaa cacttgcaaa ttctctgtta
     9241 actacaacaa atccatttgc ttctggcatt ataccacaga tacattcgaa gcatccacaa
     9301 gaagtcattg ggtcttctag tatagagtat aatgtaacag attcaactgc accttgagat
     9361 atttggttta cagtttcatt tactgaattc catacacctg ttctatcatc taagcactcg
     9421 cctttttcta ttggttgaca aggacctgtt gggtctaatt ctttagtagc cttagcatct
     9481 aaccagctaa ctgctccaca aagaccaagt ctttcaggag taacaacaca aacatgagct
     9541 ggtgcaaatg attgacataa gttacaagaa tagaaagtat caacactttc atcaactaat
     9601 gaagctaatc tttcatctct agcattgtac ttagctattg cttcaccttt taattcagaa
     9661 actttttcag catctgtaat tatagttatt tcacacttat caactactga ttcaaattca
     9721 tctaacatct tagcatataa aacttcccct atatgctcaa gtctaaatcc tttatcaaat
     9781 gcatctttag agattcttac ccaagccata tctctttgtc caacgtgcat tacaccttct
     9841 atatagttta agaagtagtg gaaacgtctt tcaagaactg gctcgaaatc ttcttgcata
     9901 tttttaccag ctattttaac tattacagca agtggtaatc ttaatacacc atctgcatca
     9961 acttcatcta tatttggtcc tattatttct atcttatggt cttcaacttc tgttgcttct
    10021 ttcttaacaa caagctccca agcttcagtt ctgtttccac caaactcagc aaacatgtcg
    10081 ctctttctaa ttctctcacc ttcaaatgct gctgcaaatg aaactgggat aggtatttca
    10141 gtaactttta tttttattcc tctagcttct aatgaagtag ctactatctt atcgtaatct
    10201 ttttgagtta ataggttcat tggaacttca agaactgttt ggtcagtcac aactgggaat
    10261 cctaaagcta ttgctccagc acctgcagat acaactaatt cacttaatgg tccaaatgca
    10321 ttaacaaatg caggaactct attagctgtg tactctaata atccgtttaa atcaccagga
    10381 gtcaatccac cgaatattaa tgctgctctt actgcaacag atacaacgtg tataacagaa
    10441 gttacatcat atcctagagg tataactctt agttcaagac ccatttttac tccagcttct
    10501 atagcttggt ctataacttt accaactaag aaagttagta aacctttaga ttgataatct
    10561 tttataactt ttgctgctga ttctgcatct ggacactcac caagaacaac tgcaacacca
    10621 ggtatatctc ctgtaactaa tggtacacca agtgatctga ttatagggtc agttatatga
    10681 cctgcacatg gctcactata tggagcatcc atagttgaat attttaatgc ttcaattact
    10741 tctgcagcta aagctgtagc taaaccagca ttaaaagcat gctctagtaa atgagttcta
    10801 tttattaaag attttactac ttctaaagca ccttctaagt cccctaaagt gttcatcttt
    10861 tgtcctgttg cagcatatat acaaggtaat gaatatgctg tatcagggaa agcaacttta
    10921 tgctcttttc cattcttttc tatagcttca gctaacatag cttgagccgc acctaaagct
    10981 tgttctgacc ctgtaaagat tatattatat agattcattc tatttccccc tttaatattt
    11041 acacctattt tttattaact ataccctaaa aaccctagaa agcaaatcca ccttcattta
    11101 actctaagaa agagtctgca tataaagatg ccccgttgat tatgtcacaa gattttacag
    11161 ttttcattaa ttctgggtca catggattca ttatagctga actaaatcca ttagccattg
    11221 ccattgctaa gaatgcacta tctaaaacag gtctaacatg cttaggacat ccattagata
    11281 cgttagataa tcctcctgta gttaaaagtc ccatctcagt catcattttt atagcttcta
    11341 aaacttctac ttgcttttct tgcatacctt ttataactaa gcataatggg tcaaatagta
    11401 tatcagttgg ctccattcct aacattaagc ctttttctaa tatttcttgg cagtaagcca
    11461 tacgctcatc attatctctt gggatacctt cttttgcaca taaacctata accattgctt
    11521 catattcagc tgctatatct attaaatcaa atcttgaacc agcatcagca gagtttatta
    11581 ttggttttgc attagttcta tcgtaaactt taagtccagc ttcgatagct tttttattag
    11641 ctgtatctaa tgctatagga acattgttga actcagattg aagtaatttg attccccatg
    11701 tcattatttc ttcgccatct ctttcggcag gtcctatatt aaaatctata tagtgtgctc
    11761 ctgcttctaa ttgctctttt gctcttttta agattggagc tgggtctctt tctgctaatg
    11821 cttttcttat tgaaggtgat atacaatgta ttctctcacc tataatcata aatttttcca
    11881 tttataatgt ccccctttaa atttaatatt aatagttagt taatatttta ttagtttgtt
    11941 tggcatgctt tttcttggta tccttttaag aacttaggta attccataga ttcctctggt
    12001 cctataacta cattccatcc tggtaagtta tcttctatat cacctttaag tactgcaact
    12061 tttcctggaa tgataaggtc tttacaatta gttacttctt ctaccttact ttctttaatg
    12121 aaagcagaga ttgagtttcc accaaattta ccagcagccc aagatgtaag aactgaatat
    12181 ccacctgcat ctggtattac taaccatact ggaacttttg atctttctat atcaccagca
    12241 actatgaagt aagttaatgc aaagtcaaca gtaactaata ctggagaatt ttcatctgga
    12301 ttgttgattg gataaatctt tggttcaact ctcattggtc tttgtgggtc agtgtatatg
    12361 ttttgtctta gtgcaaataa tggtaatgcc ttagcataac taatatcatc tattactatt
    12421 atagaaccgt attttatagt gaatattgat gataaagcaa cttccatcat agggtttgaa
    12481 ttagataatc tatttgcaaa tactattgaa ggatatccaa atgttctatc ttgctctttt
    12541 aaagctattc ttctaacttg tatagcatta gtgtaagtat cttttatgtt ttctcctgtt
    12601 acatctaata ctaattcttt atatccagca gcttgaatta attcaactgt ttcatataat
    12661 tcttctaagc taccagcttt tacacctaat ggtaagcttg ctcctttaac tacttctatc
    12721 atatctttgt agttctcttt agttgctcca tataccattg gtctagcatc ttttaacact
    12781 tcaacagctt ctttaactac ttgtgcatca tcacaagcta aaatataagc tacttctaat
    12841 tcactgcctt ttattttatc tattaatttt aaatacgcat ctttatctcc acaatactct
    12901 aaaacagcca tttcaacttt catttgttca ccaattctta catagtcaac ttttttcatg
    12961 ttagctattt tagaatctac tgcttcatca ctcatacaag tacagaatga aactgcatat
    13021 ctatttctac ttactaatgt tttttcatgt ctaaataata ctgtttctcc acctagttca
    13081 tactcagatg caccagcacc aacttttaaa gctttcatta aaggtgcagt agcctctgat
    13141 aattttgcta tagcgtcatc agacatatgt gggcatttac ctacttctac agctcctgaa
    13201 gcaaccttca tagagaaagc catacaagtt gggaatccac aatccttaca gtttttcttt
    13261 ggtgttaatt taaatatatc taaagctttt aatgccatta ttttttcccc ctaaccttaa
    13321 caatttaatt aagctaatgc gttaaccaat tcttttatag tttctattga ttttggatga
    13381 cgaagtataa ctgcgtttga accacctact aaaacacttg ctgcagttga aacttccatt
    13441 gctatagttc tttcttctgg acaaccccaa tctggctcat cttctattgg agctatagct
    13501 tctttaacat gacctacttc aaaagctact ggtgttatga taggcatttg taatgtctta
    13561 tcattttgac caaatgctgc aagtctaatt ctatccatag tagatgcaac gtactcatat
    13621 ccataaccaa ctgctgaaca tccaacattc ataactatat tttcaccttt aacgcctagt
    13681 tgagttaaca atacgtttaa ttgtttagct aggtttatat caacagaaga ttcagctcct
    13741 actttatgtg cataagccat accagctgat gctcctactc ctttgtagtt atcttctact
    13801 gctgacataa ataagcagtt atgtccatcc aatgtttgag ctaatttttc aaataattta
    13861 ccatcttttt catgatttcc tgaaccagct acaactaaag gtaattttat tgcttcaatt
    13921 actgccttag ctacatcagc acattcatca actgacttat ctaatccatt tgggtcagaa
    13981 ccatcaaatt ttaaacaaat aaaatctgct tgtgtattag cttcaacata tttagcccac
    14041 tctactggac aattagctac atctttgtat aactccttat atgaatcagt ccagctttca
    14101 ggataaacgt ctgatatttg tatacctatt tttggagaat tacctacttc tccatcaaaa
    14161 ctataaaaag gtaatacatt ttccccacct aatttaattg ctttttcccc tattcctact
    14221 tcaacttctg atatttttcc agaatatttt tgagtagaca ttttaaatgc catatttttt
    14281 tcccccttaa gcctatttta attctaattt agcaaaaatt tctttcatag ccactttaga
    14341 ttttgaatct tcaggtaaat taactaaagg aatacctgta gaatcatatt catatatcaa
    14401 ttcatccata ggtacaacac cgattagctc taggttatgc ttttcaattt cttctttaac
    14461 accatcattc ataaccccat ttggaacttt gtttacaatt agaagtattc ttccaacact
    14521 taattttagt tcctcagcta aatctcttat tctagcaaca gcttgtatgc tacgtcttga
    14581 gcaatcacta accaataaaa gtgtatcaac atgtctagtg acttttctac ttagatgttc
    14641 cataccagct tcgttgtcca tgactaagta tttgtaatga ccagatattt tgtttacttg
    14701 ttctctaagt atcccattta caaaacagta acatccttca ccttctgatc tacccattac
    14761 taataagtca tatccttcgc cttcttcaat tatagaattt aatctaaatt gaagatattg
    14821 tgcctttgtc ataccaccag ggaatgcatt gcctaacttt tctctttgat ttacttcttc
    14881 tcttatttct cctattgttg cttcaacttc gatacctaat acttcattta tgttagcatt
    14941 ggcatcagca tctacaacta atactggtcc ctttttgtcc ttaaccaaat aatcaatcaa
    15001 aagccctgtg agacttgttt taccagttcc accttttcct gcaacagcta tattgtatcc
    15061 cattttaaga cttccccctt aaatctagcc acagagcctt atgtactctg tctgtctatt
    15121 ataatttagg tacagaatga acacattctc catgtacatc atgaagtgct tccattagcc
    15181 cttcagataa acttggatgt ggatgtattg catcccctac ttgttctact gttaatccta
    15241 gatgaacagc aagtgacaac tctgtcaata aatctgtagc atgaggtcct actactgctg
    15301 cacctattat cttatcagtt tctttgtcag ctataacttt tacaaatcct tggaaatgtc
    15361 ctatagcttg tgcttttcca agccctctaa aatcaaattg acctacattg tattctacac
    15421 cttcagcttc aagttgcttt tcagtcttac caacaccagc aacttcaggc tcagtataaa
    15481 cacatcttgg aattgctcta taatctacta ccttagtctt acctaaagca ttttcaactg
    15541 ctacgatacc ttctttagaa gcaacatgtg ctaggaaagg agtatctatt atatcaccta
    15601 ttgcgtatat accttctaca ttagtttcaa gatgttcatt aactactact tttcctcttt
    15661 ccatttcaat tcctatatct tctactccag aattgtcaag gttaggtctt ctaccaacac
    15721 atactaatgc atattgtgct tctataacct ttccattaga aagagttgca acagccttac
    15781 catcaactac ttcacaagtt tgtactccaa tacctgtgat aaccttaatt ttatctttct
    15841 taaattgtct aagtagttgt ttagctacat ctttgtcttc atttaatagt atttggtcaa
    15901 ccatctcaac tatagttact tctgttccta aggctctaaa gaactgccct atctcacatc
    15961 ctataactcc accacctact attaacatag actcaggtat ctcttcaagt ccaagaactt
    16021 catcactagt tattacaact tttccatcat atgggaacat tcttggtaca actggaacag
    16081 aaccatttgc tagtattatc ttatctgctt ttatagtttc tactgttcca tcatctttag
    16141 ttacttctat agtgttttta tcaattaatt ttccaaaacc attaactaag tttactcctc
    16201 ttttttcaaa taagaactct ataccactta ttaattggtt tacaacttta ttctttcttt
    16261 ccattatggc agtgaagttt ggcttaactg taccatctat ctctatacca aaatcctttg
    16321 cttcttttac agtattcaat actcctgaag aagcaagaag tgctttagtt ggtatacatc
    16381 ctgcatttaa gcaagttcct ccaactcttc ttttttcaac aactgttaca tctgcaccaa
    16441 gcatagaagc ttttatagct gctacatatc ctcctggtcc accacctact actactatct
    16501 tcatccttaa ttcccccaaa cctaatataa tctacaatct tgcaatacta taaacctgcg
    16561 gcatccagta tagcaggtat attctcttct gccccaattg ccattatatg aacaccatca
    16621 cataaatctt cttcttttaa ttgtttgata agttcccctg ccatttttat tccttcactt
    16681 acccagtttt cttttccagc tgctcttaat ctttcgattt gttcatctgg aacaaatatt
    16741 cctggtacat ttgcagtcat aaatttagcc attcctggtg attttaaagg tactattcct
    16801 gctaatacct tgcaatccat atcccttgtt aattctctaa actttctcat tgtatttatg
    16861 tcataaagtg cttgtgtttg gaaaaattta gcccctgctt ttatcttctt ttgcattttt
    16921 aataattgaa cttctattgg agaatattct ggagttactg atgctcctag ataaaaatct
    16981 ggagaacctt ttaacttatt accagccatg tccgtacctg ccattaaagt ctcagcagtt
    17041 tgaagtatcc ccacagaatc taaatcaaac actcccttag cttgtggatg gtctcctacg
    17101 ctagtatggt ctcctgttaa tgccaacaga ttgtttatcc caaatacacc tgctgacaac
    17161 atttctcctt gtatagctat tctatttcta tctctccctg ttatttgaat aactggctct
    17221 aatccagcat cttttaataa tttacatgta gcaagagaag ttgctctcat aactgccgat
    17281 tgaaagtctg ttacatttgc tgcatgaact cttccaacta atggctttgc acattctatt
    17341 aaatgtgaaa ggtcagtccc ttttggagga gccatttcag tagtaactgc aaattttcca
    17401 ctttctaatg tttctcttaa taagctcatc tctaatcccc cttttaacct tctaaaaaaa
    17461 acttttatga atttgctgct gcttctttct ttttctttgc acttaaactt cttggattat
    17521 tttgcttaga atagtctttt ggtggtctta tttctgctaa gttatctaat tgtcctatag
    17581 cttctagtct ttcatatatc tttatccaag cacaatcatt ctctgagttt acttcacact
    17641 taccattttt agctccacca caagccccat tcataagacc tttagcacac atagttactg
    17701 gacatatccc acctgtccaa ccaagttcac agtctccaca agccttacag gcttcttcat
    17761 attctccaac tctttgtact tcccctataa acaaagtatt gttggctgga taaactggct
    17821 tattttttag attcttaact attgtctgtg taccatctcc acatgctaaa gacaaaactg
    17881 catcagcttc cttagtctcc tcttttagag cctttaaatc ctttttagac tttaaaaggt
    17941 tacaagcagg gtcaagcatt acatatccta agattttttt gccttcacct tcaagagttt
    18001 ccttcatttt taaaacttct tcttccccac cacttttaca agttgctgca cattgattac
    18061 aaccaactaa aaccagcttg tcaaaatcct tcaagtaccc tagcacttct tctaatggtt
    18121 tattttcaga aattatcata tctacttccc ccctaaacct tccacatata tatatctatt
    18181 tattgttttg taatttacaa gccttaacta catgttttgc aagtatagat gttgtaactg
    18241 aaccaacccc cgctggaact ggagttatca ttgatacttt gtctaaaact gcatttgtat
    18301 caacatctcc acataaattt ccttcttcgt caacatttat accaacgtct ataactaccg
    18361 ctccatcttt aacaaaactc tcatcaacca tcttagctct accaatagct gctattagca
    18421 catctgcctc tgctgctaca cctgatagat ttttagtttt tgaatgacat attgtaacag
    18481 ttgcatgttc acttaataaa agcattgata ctggctttcc aactaccatt gatcttccaa
    18541 gaactgttac cttacttcct tttaaatcaa cattatagtg ttttaatatc tcaactactg
    18601 ctgtaggtgt acatggtgga aatccacttt tatctccttc cattacctta gcagagttta
    18661 ttggactaaa acaatctacg tctttttccg gtgcaataac atattttata acttcttcat
    18721 ttaagtgttt tggaagaggt ctaaaacata atataccatt aacatttttg tcatcattaa
    18781 ctctctttaa tacatcaata tattcctctt gtgttatatc ctcagctaat tctaacactt
    18841 ctgttgttat acctatgttt tgacatctct ttagtgctcc tctttcataa gacaaatcat
    18901 cacttcttgc cccaactcta actattgtta gtttaggatt tataccttct ttaaccagta
    18961 aatcgacttc ttttattaat tcttcactaa ttttatcagc tacaggtttt ccttttataa
    19021 tttgtccttt agttgacatt ccttccatac attcacaact cccctacaag caaacttaaa
    19081 ttaatttatt ctatatacca catattaata acatagtatc tactttcgtc atttaaaaat
    19141 tattttccaa gtgctttttc aactttggca taaacttcat cacaaatttt tacaccatct
    19201 tcaaccaact tatctacttc tgtttttacc ttattaacat attctttatc ttgtatagaa
    19261 tttatattta taactacatt taattgtcca ccaagtatag cagctcttaa gcactgaact
    19321 cctacaccaa catcacttat agcaagtctt gaacctttat caactaaatc ttcatgtaac
    19381 ttaatagctt catagcaaac tcttactatg tttaatggta cttcacaagc aacttttaat
    19441 gccttttcca ttgtttctgc ttttattctt ttttcttctt ctgtttcttt aggaagcccg
    19501 taagcttttg ataatggtaa gaaacattct gcatcatcat ctatcatttt taataaatct
    19561 ttttctagct ttcctgcctt acttaatatc tcttttacac tttcttcata ctcagcatac
    19621 ttcttcttcc ctattgtaag gttacatacc atactaccta aagccatacc tattgcacct
    19681 actagtgcag cagctccccc accacctggt actgcttctt tagaagatag tacttctaca
    19741 aaatcaacac aagttttatc tgctattttc atgataatcc tccttgaaag gatgtaggaa
    19801 aatatctcaa attattcgag atattttacg aactatttta agatatctcc tactctttat
    19861 ttataaatta atttccttag aataatccag ctattactcc attttcatca acatctattt
    19921 tttctgctgc tggaactttt ggtaatcctg gcatcttcat gatttctcca gtaagtgcaa
    19981 ctataaatcc agctcctgct gaaattgttg cttgtcttac agttattcta aatcctgttg
    20041 gtctacctaa tttagtttgg tcatcagtta aagaatattg agtcttagcc atacatactg
    20101 gaactttagt aaatccaagt ctttctaagt tagctatttc cttatcagct tctggagtat
    20161 aatctacacc atctgctcca taaatcttag tagctatagc atttatcttg tcttttatag
    20221 ataaatcatc ttcatagcag aatctaaagt tattttcttc ttcatccaat aatcttaaaa
    20281 cttctttagc tactgcaatt ccaccttctc caccttttgc ccatacttca gaaagagcaa
    20341 cattaacacc taattcctta cacttgtctt caacaagttt aagttcagct tcagtatcaa
    20401 gtgggaatct attgattgca acaactgctg gtaatccata tacttgagtt atgttttcaa
    20461 catgttttaa taagtttgga agtccttttt caagagcttc taggttttcg ttatttaagt
    20521 ctgctttagc aactccacca ttgtatttta atgctcttac tgtagcaact attataacag
    20581 catctggttt taagttagcc attctacatt ttatatctaa gaatttttct gctccaaggt
    20641 cagcaccgaa accagcctca gttattacat aatctgcaaa gtgcatagcc attcttgtag
    20701 ctataacaga gttacatcca tgagcgatat ttgcgaatgg tccaccgtgt acaaatgatg
    20761 gtgttccttc aagagtttga actaggtttg gttttaaagc atcttttagt aatgcagcca
    20821 tagcaccatt agcttttaat tgtctagctg ttacaggttc accttcgtag ctgtatccaa
    20881 ctattatgtt tcctaatctt tcttttaaat cagatatatc acttgccaaa cagaacgctg
    20941 ccattatttc agaagcaaca gttatatcga atccatcttg tctaactgct ccatcacctt
    21001 ttttacccat accatcaacg atatttctaa gttgtctatc gttcatgtct acacatcttc
    21061 tccaagttat tttcttaggg tctattctaa gtgcatttcc ttggtgaata tggttatcaa
    21121 gcatagcagc taaaagattg ttagcagctc ctatagcatg aaaatctcct gtgaagtgta
    21181 agtttatatc ttccatagga acaacttgtg catatcctcc accagctgct ccacctttaa
    21241 caccaaatac tggtcctaga gatggttctc ttaaagcaac taatacattt ttgtctaatt
    21301 tagcaaatgc atcagcaaca cctatagtag tagtagtttt tccttcacct gctggagttg
    21361 ggtttatagc tgtagttagt attaacttag cttttttacc agtctctctt tttagtagat
    21421 tgtagtcaac ttttgctttg tatttaccat aaagttctac atcatcttca cctagtccta
    21481 atttttttgc aacctctcta atatcttgag gcttagcttc ttgagctatt tcaatgtcag
    21541 atttaaatcc cataactgaa tctcctccca caacctaaat agatttaaaa tataattttt
    21601 gtaaaaattg tattaatatg taaaaattat attttataag ataattatac atgtaaaatt
    21661 acttaaaaac tagctactaa gctattaaaa tattcaaact taatagctag ttatttatct
    21721 aattacttac ttattaccta tctattgcct atgtctaacc tttaaccaac ttaaaatccc
    21781 aaatcaaaaa ttctatctat tttatttatc cttaagtgac attaatttat cttttattgc
    21841 tttaatctgt tctttcgttg tttcactaga atcatatgga gattgattag ctctatcaga
    21901 atcaataaca tcttggttat aataaataaa tcctaaagat tctccatctt ctaagttttg
    21961 gattataaat tcttcatctt ccttatttct aattttattt cctactacaa atactttatt
    22021 tacacctata tcatgtccaa gtttcttaac ttttctataa gtctgtagac ttctctctcc
    22081 tggttctaca actactataa aagcatctac accttgggct gttcctcttc ctaagtgttc
    22141 aattcccgct tccatatcca taaccactac atctttattt tgtagtatta aatgtgagca
    22201 gagtctcttc aaaagaacat gctctggaca tacacatcca gtaccaccag aatctactgt
    22261 tcccaatgta agtaatctta ctccattgta ttctttacag aaattttcag ggatatcgtc
    22321 tactttagga ttcatcttaa acatttttcc aaatgaacct acagaagcag ctgttctatc
    22381 tgaaaccaat tttttcattt ctgatatagg tactattgat tcatatactt cttttggaaa
    22441 acctagtgct aaagctaaat tagcatcagg gtcggcatca acagcaacaa ctctgtatcc
    22501 gtcttcagca aacatcctag agagcattga agaaaaagtc gttttaccta ctccaccttt
    22561 acctgttatt gctatcttca taatccctac caccttttat tactatttgc tttttagtag
    22621 ttaattttac agttaaatat tttttatact ctccattttt atgcactaaa aataaactac
    22681 agctagtcct tacaggaacc aattagattt accctactag cttttcaaag cagcatgtat
    22741 agttttatta ttctttaata ttgtttatat tttatttatt cagtatattc attatatttt
    22801 gatgttcttt actttaatat ttgactttat tcaaccagaa acttcttata aaaacgttta
    22861 ttctataact tcgctatttt ttaagcttca gccttttctg ccattttttc ttcaactaat
    22921 gcttcaaagt gcacacgttt cttttctata tcttctatta ttgtagctgc taattctttt
    22981 ggatttgtat ttacagtaaa tgtagctcca actttatctt ttaattcatt agttaatata
    23041 tccactgctc ttgaagaacc agctattgga ggcattattc ctaaataagt gtctatacca
    23101 gaagctacaa catagcatcc tatagcaact gctttttctg acatccactc tggagctata
    23161 cctacaactg gaaggtcaca catatccata tctaagtaat tagctgctgc tccaactaaa
    23221 tctagtatac ggcttatatc aacacaagaa cccatatgta gtactggtgg gattcctact
    23281 aatttacaga cagttgcaag acctttacct gcatattttt cagccgcatc agattccatt
    23341 aaaccaaact tagctgctgc tgaagctcca caacctgtag taactactat tatgtcgttt
    23401 ttgattaatt ctttcattat tgtcacgtga gcctcatttg aaactccttt agcattgtta
    23461 catccaacta caccagctgc tcctcttaat actccagctt ttagacagtc tgttaatggc
    23521 ttaactgttc ctgctgggtc tatatgagag tttacaactc tgtctaattg atttactact
    23581 gcttctacac tatacccaac tgttgcccct gattttaatt caggtataag tacttttgat
    23641 ttatctctat ttttaaagtt taaaatagct tctttaacta ttgctttagc atcatcaagt
    23701 gcttgttctt ctctaaattc aatataagtt gaacctgtta tcttagcttt tggagaagtt
    23761 gttacaaatt tagtatggta acaatctgca actctagcta atgcagggaa tatacattga
    23821 acgtcaacta ttacagcttc aactgctcca gttattattg caagttcttg ttgatggaag
    23881 tctcctgcta tttttacccc atgtctcatt gtaatctcat tacctgtaca acacatacct
    23941 gctaagttta ttccatcagc accaacttct ttagctaaag ctactaagtc tggctcttct
    24001 gatgctaaaa ctatcatttc tgataatgct ggctcatgtc catgtaatat tatgttaact
    24061 ttattttctt ctaatactgc taaattagcc tctgttcttc ttggcactgg agttccaaat
    24121 aagatatcgc ttagacgagt acctatcata gaaccacccc aaccatcagc catagaagtt
    24181 ctaagactca tgtgtattaa actatctata tcaccagtac atcctatatg agtagagtgc
    24241 attattgttg caatttctct atctatagct cttggttcta tagcatattc tttccatatt
    24301 ttttgtcttg gttctggtgc attttttata aatcttaatg tcccaaaagg ttttccaaat
    24361 tccattaatg caatttctgc cacttcatgt gctacatcat agatatctct accttctgtc
    24421 tctacatccc attctttagc taaagtaatt aacttagctt cgtctcttat tttatagttt
    24481 ccatttggat cagctaatgc taatgtatgt gctatatctc tagcatggtc tgaatgggca
    24541 gctgtcccac cagcaaccat tcttgcaaag tttctaccta ctatagtatg ctcatctgct
    24601 ccacataacc cccttggagt cttagggctt attctacatg gtcccattcc acatattcta
    24661 cagcaaacac cttgaagacc aaaaccacat tgtgccttaa agccttcttt tctatcatac
    24721 attgtttcta cgccttcttc tctagcttta gctatcatct tctggctgtt taaatcaatt
    24781 gttaacattt tttcatccat attcctatcc cctttttttg atttttaaaa tcccccaggt
    24841 gactgacttc atgtcataaa ataataaaat aaatgctttc taagttgatt atatactaat
    24901 tttcagtgct ttgctttaga tttaaatatt tttttaacaa aattagataa tacataaaaa
    24961 ttttattttc tacacttaat ttaagttgat tttatcgctt gtttttggct atttgttaat
    25021 atctaaacct acttttttga tacttcagtt taattaaatt atgttttgat ttttttaaat
    25081 caaatttgaa atttttctag tcatttttat attattgcag attgtttgtg tatttattat
    25141 ctttcactac tgtttggact tattacagat ttttactccc taaacttaat tagtaaaaac
    25201 ttcttagaat agcatttttt ggactaattt ttttgaattt ttttattttt cctactttaa
    25261 tattaattat agcttcaaat tgaacatttg tatatattca tttgaagcta taattatttt
    25321 ttatataaaa cttttttaat acagcattaa tttagacatt ttgactattt tttctacttt
    25381 aattcattta tttcaaacga tttatcctat ttcatatttt tagccacaat agcaccatat
    25441 gcaatagaat ataatttaga ttcatgtgaa tcagctcttg acactaaaac tataggggct
    25501 tttgcaccca taattatacc tgctgattta gagtttgcca tatatgtaaa ggctttccct
    25561 attccattac caacctctat tgttggaacc aatatgatat ctacatctcc agatacttca
    25621 cttttaaacc cttttatctc actagcttct tttgaaacag ccaaatcaaa agccagagga
    25681 ccttctacaa ttacattttc accaaattct ccttctttac aagcttttgc taacatatct
    25741 gcatctacag ttgcttgcat cttaggattt actttctctt tagctgccaa gcatgcaact
    25801 tttacagttt ccattcctaa agcttttgca gcttttatag aattttttaa tatctttgcc
    25861 ttttgttcat aatcaggagc tatattcata cctccatctg tagttataag taatttatgg
    25921 tatttttcta actcatatac cataacatga cttaataaac tatcagttct aagaccatag
    25981 tctttattta acacttcttt taatatgaca gatgtatcta ataacccttt cattacaaag
    26041 tctgcttctt tacttgatac taatttaact gctatttctg cacactcttt gatatcatcc
    26101 ttatccacta ttttgatacc acttaaatca aatcctattt cttttgatat ttctcttatt
    26161 ttgccttcat ttccaaccaa tataggagta attatatttc tttctacagc atcctttatt
    26221 gccattaaaa cttctttatc atgagctgct gctacagaga gtacaatttt ctctcctccc
    26281 ctaagttggt ctaaaatatc atctaatttt tttatcaaaa gtaacccctc ccttatatat
    26341 atttaactaa ataataccat atttgctata ccttttctca atttattttt tatgttataa
    26401 agaatttata taactaaaag atagtttaat attttattcc gggatttcat caataactct
    26461 taaaagcttt gcaggaacac cacctacaat agtattttct gcaacatttt ttgttacaac
    26521 tgctccagca gcgataattg cattatctcc aatagtaact cctggaacta cagttacatt
    26581 agcaccaatc catacatttt ttcctattgt gattggagaa ggataagttg taccacgttt
    26641 ctctggtgca acaccatggt taagtgtagc caataccaca ttatgcccaa tttgcgtgtt
    26701 atccccaatc tcaatccccc cctggtcttg aaaatggcaa ctgctattta taaaaacatt
    26761 ttttcctatt gtgatgtttt ttccacaatc tgtataaaat ggtggaaaca taaaaaatga
    26821 ttcatctact ttttttccaa ttaactcaga aaataaacta cggacttctt ctggctcatg
    26881 ataagaagaa tttaacttac ttgtgatttt cattgcttca ttactcaacc taaccataaa
    26941 ttcatgtatt tctgatttac taaaaacagg ttttctttga ttgacatgtg ttaaaaattc
    27001 attgatattc attttatgca tcctttcaat atacgcttta atttatttgt acattctaaa
    27061 tgatatttta ataaaaatta atttaattta tttttattaa aata
//
LOCUS       DAEPXF010000037        26925 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain HB27
            SAMN13376389-rid12479423.denovo.039, whole genome shotgun sequence.
ACCESSION   DAEPXF010000037 DAEPXF010000000
VERSION     DAEPXF010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13376389
            Sequence Read Archive: SRR10518432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 26925)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26925)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001069624.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001069624.1
            Genome Coverage       :: 315x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/15/2021 01:54:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,165
            CDSs (total)                      :: 4,076
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 89
            rRNAs                             :: 10, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 10 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 19 of 69
            Pseudo Genes (incomplete)         :: 44 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 10 of 69
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26925
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13376389-rid12479423.denovo.039"
                     /strain="HB27"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="China: Hebei"
                     /collection_date="2017"
     gene            complement(132..701)
                     /locus_tag="KRL67_001383"
     CDS             complement(132..701)
                     /locus_tag="KRL67_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HBH1522278.1"
                     /translation="MSYMPTLEQAWEILRKYNKEEFHIRHAQIVSGVMRYYAKEYDPE
                     RVEFWEIVGLLHDLDFEMYPDEHCVKQRELMTELDLDKSIIDSTISHGYGLTGSDVKP
                     EQFMEKVLFAVDELTGLIGAAAIMRPSKSVSDLELKSVKKKFKDKSFAAGCSRDVIRS
                     GAEMLGWELDKLIQTTIEAMRSLIPEMAI"
     gene            complement(862..2112)
                     /locus_tag="KRL67_001384"
     CDS             complement(862..2112)
                     /locus_tag="KRL67_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBH1522279.1"
                     /translation="MVKSLNTFNKNFNLMILGQIISLFGASILKFALSLYILDITGKA
                     EIFATILAVSSIPIIIFSPIGGAIADIFNRRNLMVIFDFSSSITVLILALFLFNNNGS
                     ILLVGIIMTILSVISTMYQPTVQSSTPLLVDNEHLMNANGIVAGVASLTNIAGPVLGG
                     VLYGVIGINAIIAISCVSFFLSAIMEIFIQIPFIKQEQTSNIIKTIFTDIRDGIVHIC
                     KENRFILKILFLAIAINLFLSSMLIVGIPYIVKITMGASNFLYGLAEGAISFSSLVAA
                     VSIGIFSKYLKITNLHIPFIICALVFIPMAFSVYPFILNTGFPVPFLIFIVSSMLIFF
                     TTTLISIYVITVTQLKTPNELLGKVMSILFSGTAIAMPLGQVMYGKLFDLLSDRVYII
                     ILGVGFITLLIAFVSKITLRNEKL"
     gene            complement(2121..2354)
                     /locus_tag="KRL67_001385"
     CDS             complement(2121..2354)
                     /locus_tag="KRL67_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH1522280.1"
                     /translation="MIELIQKLNDVIYKINDKLNIHTKLVDPSSELMPLYSMLSIIVS
                     IFVIILGIKVRKKVLIVIAIIAIIVNVLIILDR"
     gene            complement(2405..2983)
                     /locus_tag="KRL67_001386"
     CDS             complement(2405..2983)
                     /locus_tag="KRL67_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBH1522281.1"
                     /translation="MAKSFTEIEKDNIRKRLISECEINWSKYGYKKTNIDELCSKSGI
                     AKGSFYTFFDSKEKLFFEVLNGIQNRLISSVDEILGNMPQKEGFAKALKMLYRLYDKN
                     PFLYSPNNTDYITLLNKIPKEMLSQLEYNNVQSFYSLIERHNLKLKIEKEKAFGVCNA
                     LLSSLLLKEDIGYDYIEVFDFMVDNLVEHILE"
     gene            3284..4744
                     /locus_tag="KRL67_001387"
     CDS             3284..4744
                     /locus_tag="KRL67_001387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent glyceraldehyde-3-phosphate
                     dehydrogenase"
                     /protein_id="HBH1522282.1"
                     /translation="MFNELRTKENVYKNLINGKWVESNSRKPIEIYSPIDNSLVGKVQ
                     SMTKHEVDEAIKNTKESIKVWADMPVYKRANIFHKAADLLLENIDEIANILVLEIAKD
                     IKSARAEVERTADFLRYTADVGKNMEGEAISGDNFPGGTRNKMSYVSRVPLGTVLAIS
                     PFNYPVNLSMSKIAPALIGGNAVVLKPATQGAISALHVVEIMRKAGIPDGVLNTITGR
                     GSEIGDYVVTHKGINFINFTGSTEVGQHISKISGMVPLLLELGGKDAAIVLEDADLDF
                     AAKNIVSGAYSYSGQRCTAVKRILVQESVADKLVGKIKPLVEKLTIGNPMDEVVITPL
                     IDNKATDFVQGLVDDALHKGAKLITGNVRKNNLFYPTLLDNVNVDMKIAWEEPFGPVL
                     PIIRVKDINQAIEIANQSEYGLQSSVFTSDIDRAFYIADKLEVGTVQINNKTERGPDH
                     FPFLGVKASGMGTQGVKYSIEAMTRPKAVVVNVREL"
     gene            5129..5734
                     /locus_tag="KRL67_001388"
     CDS             5129..5734
                     /locus_tag="KRL67_001388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002591427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBH1522283.1"
                     /translation="MPKGIVLTPEQQAERREKIVAVALSLMAENGFQKTSMREIAVLA
                     NMGKSSLYDFFKTKDEIVVYAVEKEIEKTIQQVHRIIDGEPSPQQCLRKIMLNHLRFP
                     EQYRTVLMWLNTESDYLEEEYRKRLKDARYAYQDIIKSVIDNGVTTGIFRKTDTALVT
                     RLLINSMLSISYTSRPSDSSEKMLDETMNIFLHGIMNDGGE"
     gene            5737..8304
                     /locus_tag="KRL67_001389"
     CDS             5737..8304
                     /locus_tag="KRL67_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002602373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, phosphate dikinase"
                     /protein_id="HBH1522284.1"
                     /translation="MIYYVLPLEHKQATIEIVGGKGMSLSKLLTAGISVPDGFHVTTA
                     SYQIFVETNHIQSRINKLLDGIDSNNTSQLEDISKKIGVLFHNGEMPQEVSDAIKTAY
                     AGLGNISVAVRSSATAEDLPDASFAGQQETYLNIQGEDEVIDAVKRCWASLWTARAIA
                     YRVKNNIKHEIVALAVVVQKLAFSDSSGIMFTLNPINGRRSEMIINAAWGLGEAVVSS
                     LVTPDTIVVDKDSERIISYEVANKEIMTVRTSEGTEETMVPERLRKKYALTRNQVMQL
                     IQLGKKIEKYYQMPMDVEWALEKDKLYIVQARPITVLPPEWVLPEQDVVYTKGSLAEH
                     LPNPVTPLFATLGLEIVNRASELLWIDMFGKSAKKLLPKNGAYTVINGYVYLSAKSKP
                     LLIAIKSLSPRSLRRTLMNSVARWETARKGYEDAIKQWEEKPLHTMNAHQIMEGIQTI
                     FYAACIYFTRIQLTLPAASISETLFTKLFQGAVRRAGITDTSVFLLGFDTIALQSEKE
                     LWGISEWVKQNNSINLYLQNSSAIKIAEDFMSSVVPVGVSQEVWIEWKNRINKYFKEF
                     GRTAYEFDFAYSTPQETLTPTFESVKTFVEGKGESPFLRQTTFEKRRKHAEEEILQHI
                     GGLRKKLFFKLLHWAQETSPMRENAIYLMGMGHPLIRCMFQEISERFIQGGAISHLDD
                     IYWLTKSELEALIEQLDKNRSLSDMNGLIPVRKAELKKYMGYVSPSKLPEKNKKTISQ
                     ALQKQKDGKIVLTGIGTSTGVVTAPACVLNSPADFESFQPGSVLVAVTTTPAWTPLFS
                     SASAIVTDIGGPLSHSSIVAREYGIPAVMATHIATRTIKSGQMITVDGLAGTVTFDE"
     gene            8297..8461
                     /locus_tag="KRL67_001390"
     CDS             8297..8461
                     /locus_tag="KRL67_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH1522285.1"
                     /translation="MNKKAILALKLSTFVIALAEVVILKVLRFNHTTNTHQTAVRKTI
                     AWWGFSYAKK"
     gene            complement(8564..10648)
                     /locus_tag="KRL67_001391"
     CDS             complement(8564..10648)
                     /locus_tag="KRL67_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HBH1522286.1"
                     /translation="MSKYIDFANIEYSLLMNALDVSVSKHLLDEHFTVVWSNDRYYEM
                     FGYTKEEYETLFHNQCDLFYKDNPEDWNELVEYVEKTFSTGAKKYDYVCRMKHRNGKK
                     LWIKLFGFLTGEVVNGYPISYSVMIDITEQMMIKIEQTVTYNNFPGLIAKFKVTDFGF
                     QLLDANKKYFDMFKDNVNFLLDDINLESYLSAVYKYQSDFKAGKYVCFSVSPQNINGD
                     IVHMQVVAECIDWEDNKPIYLLIYSDVTKLVKQNEIIEKNNKELKKLAFIDSVTGGIN
                     RTKFNIIAKEIISKSPSGTYTLIWLNMKKFKLINDLAGNIAGDNLLKYIYNCISKHLH
                     EGELVTRIFADKFILLLKDKPEDITSRLNSMVTHINRFNNNADYKYILYFTAGAYVIY
                     DTSLDITNIQDSANVASKSIKNVENHGLCICNFYSKKDKEKLILEQSIENRMYEALEK
                     REFQVYLQPKLELSTQTICGAEALVRWIHPVEGIIPPNEFIPLFEKNGFIIQLDLYVF
                     EEVCKTLKKWIDDGLNPINISVNMSRYHFSSNNFIKTYDKIAKKYGIPSHLIEIEITE
                     TIVFEDVKVFSEIINEIHKNGFKCSIDDFGSGYSSINMLKDIYVDTLKLDRAFFQSEH
                     LDSQRERDIIVSIIELAKKLKMTTVAEGIETSFQKDFLQEINCDMLQGYVFSRPLPIK
                     DFEKLMFQNSSN"
     gene            complement(10696..11577)
                     /locus_tag="KRL67_001392"
     CDS             complement(10696..11577)
                     /locus_tag="KRL67_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HBH1522287.1"
                     /translation="MPAYLFDSNSITNIYRVFSILLSFIVTGLYFSYKNHKNNKINFE
                     AVLEDKKNLIFLLSLMFGMYLYLCLSLSIYYTVGNVLILKLYGIKSAIIVLVGNYIAE
                     RYSFRITQIKGYDIKNQIEYVESIKTEYEINKLNDIVYTDLLTGFYNRPFVNQKLSEW
                     TSNHYKFTLCFTDLNSLKPVNDKFGHQFGDKYISTTAQIIKKLINVENSLLFRYGGDE
                     FLFLLQNTSCIEAEKIMIDINYELSKLSNTDEYPYALSISYGLVEWDGISDIETLIAE
                     ADSLMYENKLKNKSTLN"
     gene            complement(11927..13105)
                     /locus_tag="KRL67_001393"
     CDS             complement(11927..13105)
                     /locus_tag="KRL67_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HBH1522288.1"
                     /translation="MKYHIFLIITTILIIELCNIYICKIASFGDKNITIHKHYKYFFL
                     IKPLTFLIIIFTKLPLPLYYILFYILNHKLLLSRKSSTNNHFIPNIAVISFCILHLIT
                     VSTMSLVKNTSPDIVSVNMSYKLISILVASILDFILWYYMDKIKEHIYIITQNQIEFS
                     LFLKFIWFTLGYILFDSIQITFAPPSSLTSYFILGSNILLILQLIIFINHIYSIEKNI
                     YLEKEHNRLKMIKQEQLLKVNLLKKKANIDDLTSAYNRKFAFEKIQHYIDNSLLFSLI
                     YIDLDKLKTINDTYGHLSGDNYIINFSSIVIENLGIDDIFARIGGDEFIVIMPKSNEN
                     TTKEFIEKISKIMDNKISFSYGIVEVPSESKMTVEEIINIADIRMYTNKQNNHKRNNN
                     "
     gene            14005..15444
                     /locus_tag="KRL67_001394"
     CDS             14005..15444
                     /locus_tag="KRL67_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBH1522289.1"
                     /translation="MKRKPQYLIIFEHIIQQIYTGKITIGDRLDSIKSMSEEYMVSKN
                     TIKTVIKMLSEKGFIETKVGSRPVLIQSTSKQSIESDLLYEKILQISEIYKIFSLIFP
                     SIAMYNVKRFTSKDFEELNEIIDCMEKNSSNYLIFREQRLKYINKLISKTNNFLIYHF
                     CEIIQHDMIISQLAYSFDNLDNCCIDFFRNKYIEQIRKTYLYGLDGNLIELKKLLTYI
                     YKACRESILKMVENRAPQSRDTSSFNSVTLEKCYLYDLIVSDVICQIFEGNIKIGDCL
                     PSITSIINRYNVSLPTVRSAYNELNELGIAKTINGKGTYITLFSEYDDDYINTEEGAK
                     RLRLLLDAMEFVTITLEDVVFLLGNKIDLIAVDKIETHLRYLQNNCKECKVYPDFVLL
                     CKIVDYTQIYILQETFLHLKQYMIFGIYLERFFHEEYNEILKTRFDVCFDILRYLKKG
                     DVESFAESFSNIFKESLQYLKKVISSLNR"
     gene            15757..15936
                     /locus_tag="KRL67_001395"
     CDS             15757..15936
                     /locus_tag="KRL67_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH1522290.1"
                     /translation="MGKDRFEKVYSQGKINVMEIWVDKETGVNYVFHYYGNAAGFTPL
                     LDREGKPVISPIVSK"
     gene            16555..16830
                     /locus_tag="KRL67_001396"
     CDS             16555..16830
                     /locus_tag="KRL67_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH1522291.1"
                     /translation="MSLFVGGWSKFRVIEEEDLKVFNEAVGMLKGVDYEPLIVATQVV
                     AGTNFKFICNATSVTNPPQNYLAEIIVFKPLPCDGHNATITSINIIK"
     gene            16850..17137
                     /locus_tag="KRL67_001397"
     CDS             16850..17137
                     /locus_tag="KRL67_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH1522292.1"
                     /translation="MRLNVTKQNTIEVENSRKEDVYFVIVDNVFFERQGDVFNSITNN
                     NLNNYDVKILPTDNVFLELKITSTKDLEPVFIVVEDRQYNHGSIMFKPVLV"
     gene            17654..20149
                     /locus_tag="KRL67_001398"
     CDS             17654..20149
                     /locus_tag="KRL67_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosome-binding GTPase"
                     /protein_id="HBH1522293.1"
                     /translation="MPENNNQLLEEITLLLNKGKAAINKAENQNLILILGGTGCGKST
                     AINYLAGCVMREVEDEDTGISYIECLDPVAEIGSGAVSQTLYPNIVNMRKPIEGMDAK
                     LCDTAGFSDNRGVAHDISAAVSLGKVFKNSASICAVIVLIPEADILDSRLTKVLDLFQ
                     QLNMCLHKENFKDSITFFISKSFLGRTEKQIYNVIKRKYEQLCENLDSNIEEKAWLFE
                     ELLKDNGKNIHLCKPLASDEREGLLKQVLELDEVKDKTSAFQYPISADARLVLETLIK
                     EIQNNIVSSLEDYVKAYTEDFISIIGSITMIQEIAKYDEVVKVFKNWLESTTSLTIDR
                     FINVLAKQSGLFLQDSCMKLQNKIKGNLDIIDALSKYTEHGTGEILLPSVHMEFIIEK
                     ASKKIMEQNFIIIRKNLEKVLVSYEVQKQVFTLPNREKLMEVANKKDYVYSVSDFNSI
                     LTNVTVTQNEELEDSITRLSVYGEEGNEIIREFIGKYILNPFIVKTININDKKGIEIR
                     ALIPNLVISSVLEYLKNRKVDDNVSTFMFFAKDTIYMDDNLGMDFASEKNIILACNNL
                     DVGSNVKINVSGKSGELVDSSMYGKNGEDGRDSGNIYLYIKEEIRNYRLTLIANGGNG
                     SKGIDGKNGETGASGRNGADAGYTSYFHGQMFKGYFVKGTSGKKGEKGGAGGNAGRGG
                     NPGNKGNIYFISDSEEQKNRIINHCDITVKDGEAGPNGRPGLGGYGGSGGYRGYNTLS
                     FSPSGIDKTVYYTGYFTSWDYKLDGITKYHIEFSGGSWNRGKTGPAREYAEYGDRGDN
                     GGFNAPNTKLSTAVSKVEENIIKEKYQGYCSEI"
     gene            20164..25344
                     /locus_tag="KRL67_001399"
     CDS             20164..25344
                     /locus_tag="KRL67_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosome-binding GTPase"
                     /protein_id="HBH1522294.1"
                     /translation="MANKLYSEIINLLEEGRDEFRKYDLKEKSILLFLGASGVGKSTC
                     INYLKGCVMEEKTDEETGQIYITAKDSAVEIGNGVYSKTLCPEVVDIANRDFSLCDCP
                     GFFDNRGAEYMIAGAMLVRETISTSSKVKGMVVILDYQSLLDSRHTLLIEIGKNLKDM
                     LGDYAKYKENFFFLINKIPKALIGNVTEEKIRNILKKAKEDLDSQEKDYDGYKELVYL
                     LNVALSDGVSIKLCNPLDKQPEKLLDEMKNLPETFEKTDMGLMLTASCKIEIQKAVDV
                     TYKDLEKLLVPYIESVKKGMEDSISILSSINSLKDKKIQLDKMYTDLCSRTNNMKELM
                     EFVQELSYGDGVQKVNILEETRLLLELNSYCSETHIVGLSELLKSLLKNTLEDTLRDI
                     KYQIIEEETKEEESKKLEDAEYRIEKIQSCTYIKSYEKENVLSIYIPIPKVNLSDLTK
                     IISGYQLNILSSIYMYGDCELKIDCNMEKEVFSGKNIVLLTEKVFIPEGTYSINVSGK
                     DAEKLVGEDSIYNGMNGRNGNNAGTSGDILLSFKEIEKRGELKLYADGGQGGNGEDGK
                     IGATGLTGEMGANAKRDSKRESKAFVENYESSFFMIKAEKGKQGGTGGTGGNGGNAGI
                     GSKSGQIYLIGKAIVLKRNISKKDGSHGMAGKGGSGGKGGSGGLGGIRGIFEVTKTFD
                     KYNINYYFFDRCILDGGKEVPDDGEKLKALYEKHKDWTISKREESGSYGEDGKEGRFG
                     DALKERELLKQEYSLSMDTFFYEFLERFGFSGSKGYRNLRLLCAVGILEADILELSHI
                     YEIFHAVEKVEMQLNREILQKYSGFDKKNKAVISLDDMNIKKEFYKEAKEIYEYLINK
                     LMQSDRIESFIGEKQNFSEWSMILFALEQKVAFYVQMIEENEIGGNRVTDIRSLTEYL
                     DKILIELEQGKKDELKEGYFNTYKESILTQIKYGNQQIEVLKKELENKMLSVNAKMNL
                     LIQEIIKIKENEKEDIEKLKEKQKKLKKQMKKQLILSGIQSALGLLTAFSGVGQACLG
                     AFDSAKSLQENLSAKEEECKWTNTMCDFSDFKLNFDGIDFVVKKQLKEDRKEALGKMT
                     YNLAGIDGVTEVKFDQIKAIMVKSIEDNNYANKANDIQIAKDGLNEGKYEEIYSKIQK
                     KEIGILEANYNNKQQKDKLDALRKSKEAIERQQKISTIKNALSIGSSACFLVNTAISG
                     VNNCLNVKSSFESKIEVVKEAIKDKENNIKKLDELNTQMAEFKDKTFKESIIPFVEKF
                     DDTVKNQDIFENEISKLKMKEVLTELSTKFASLKMENSEDISIIFKKVQDMMETQIII
                     TDKIDKKKDNLAIGELIYQISDSGNQINEYEKKLIHQIELNQVRYLCQVEAAAFQLWT
                     FPFGSNIVNILNRCHVGITEDENEVVNQAKEQNRQIKEFLDQDVYQWQKTDNHILKVA
                     LTGSLDGKSRVGFENTYSLYDCNEEEVKKLLTGDEISFEVPLNQNFDAIKYVTMYANI
                     PNLRRNILEDSLKVKVHIHLYDSGYFSLYDDKKDVYYQYVFPQNYIDITHGLDVKYND
                     SDSINSIWSSVEDKGIEKFKNAGLNSGRSPYSMAKISLKAHFNKKLSYDSLTNEQKKK
                     LESYFDKPSKYAWSDSIDILKSLGYIDLKGILSKSWYEIKKDIDSDAEIMKHFSILQV
                     DLLERIIYEQTSILKENIPNLTIELVGCGIYVESEFNYLKQNDLKCYEERLVENIINI
                     "
     gene            complement(25549..26490)
                     /locus_tag="KRL67_001400"
                     /pseudo
     CDS             complement(25549..26490)
                     /locus_tag="KRL67_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729454.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
     gene            complement(26608..>26925)
                     /locus_tag="KRL67_001401"
     CDS             complement(26608..>26925)
                     /locus_tag="KRL67_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBH1522295.1"
                     /translation="IEDLQVKNMIKNHKLSRLISDVSWSEFIRQLEYKANWYGRQIVN
                     VGKFFASSQICNKCGYKNEEVKDLNIREWICPSCNETHDRDINAGMNILKEGLRLITI
                     QNK"
BASE COUNT         9976 a         3414 c         4527 g         9008 t
ORIGIN      
        1 agtatttatt atagattata tagtgtgttt aaaacattaa actttttatt ctacaaaata
       61 taaaaagaag ttgcatcaaa aaaagaaatg ctctttaaag atgtaacttc tatggtatat
      121 gtatgaggaa gctatattgc catctctgga atcaagcttc tcatagcttc tatagttgtt
      181 tggataagct tatcaagttc ccatccaagc atttctgcac cacttcttat aacgtctcga
      241 gaacagccag cagcaaatga tttatcttta aatttcttct taacagattt taattctaaa
      301 tcagaaacgc tctttgaagg acgcattatt gcagctgcac caattaaacc agtaagttca
      361 tccacagcaa ataatacttt ctccataaat tgttctggtt taacatctga accagttaat
      421 ccatatccat gacttattgt agaatcgatt attgatttat ctaaatcaag ctctgtcatt
      481 aattctcttt gtttaacaca atgttcatca ggatacattt caaaatctaa atcatgtaaa
      541 agtccaacta tttcccaaaa ttctactctt tcagggtcgt actcctttgc atagtatctc
      601 ataactccag atacaatttg agcatgtctt atatgaaatt cttccttatt gtattttctt
      661 aatatttccc atgcttgttc taaagttggc atgtaactca tttttatcac tcctctagct
      721 gaatattttg aatttttata aagtttattt tccattgatt atatcaatca atttccaaaa
      781 cgtcaacata ctgatacgca attttgtatt taaattcaga aatcagtgaa aattaaatct
      841 ttatgtccaa attttttgta tttaaagctt ttcgttcctc aatgttatct ttgatacaaa
      901 tgctataagt aatgtaataa atccaactcc tagtataatg atgtacactc tatctgataa
      961 taaatcaaaa agtttaccat acataacctg accaaggggc attgcaattg ctgttcctga
     1021 aaaaagaatt gacattactt ttcctaagag ttcatttgga gtttttaatt gtgtgactgt
     1081 tataacatat attgagataa gagtcgttgt aaaaaatatt agcatagaag atactataaa
     1141 gattaaaaac ggaactggaa atcctgtatt taatataaat gggtatactg aaaatgccat
     1201 tggtataaac acaagtgcac atatgataaa ggggatatgt aaattagtga ttttcagata
     1261 ttttgagaaa ataccaatac tcacagcagc tactagtgaa ctaaaagaaa ttgctccttc
     1321 tgctagacca tataagaagt tactagctcc catagtgatt tttactatat aggggatacc
     1381 aactattaac atagagctta aaaataagtt tattgctata gctaaaaaaa gtatttttag
     1441 tataaatcta ttttctttac atatatgaac aataccatcc cttatatcag taaatatagt
     1501 ttttataatg ttgctggttt gctcttgttt gataaaagga atctgtataa atatctccat
     1561 aattgcagat agaaagaaac ttacacaact tatagctata attgcattaa ttccaatcac
     1621 accatacaat acacctccca atacaggtcc tgcaatattt gtaagggatg caactccagc
     1681 tacaatacca tttgcattca ttaaatgctc attatcaact aaaagaggtg tacttgactg
     1741 aacagttggt tgatacattg tgcttatgac tgataatatg gtcataataa ttcctacaag
     1801 taatattgaa ccattattgt tgaataaaaa gagtgctaag attaacactg taatactgct
     1861 tgaaaaatca aaaataacca ttaagtttct tctgttgaat atatcagcaa tagctccacc
     1921 aataggggag aatattataa ttgggattga agatactgct agtatagtag caaatatttc
     1981 tgctttacca gttatatcga gtatgtataa agacaaagca aatttaagta ttgatgcacc
     2041 aaataatgag attatttgac ctaatatcat caagttgaaa tttttattaa aagtattcaa
     2101 agattttacc atatatatac ctacctatct aagattatta aaacatttac aataattgct
     2161 ataatagcaa ttactataag gacttttttt cttactttaa tacctaatat tattacaaat
     2221 atagaaacga ttatagataa catagagtaa agcggcatga gctcactgct agggtctaca
     2281 agttttgtgt ggatatttag tttatcattt attttgtaaa taacatcatt tagtttttgt
     2341 attaattcaa tcatattacc ccttctagtt ataaagaaat gtttagaaaa tttattttaa
     2401 attattactc aagtatgtgt tctacaagat tgtctaccat aaaatcaaat acttcaatgt
     2461 agtcatatcc aatatcttct tttagcaata ggcttgacag tagggcattg caaacaccaa
     2521 aggctttttc tttctctatt tttaacttga gattatgtct ttcaattaag ctataaaaac
     2581 tttgtacatt gttgtattca agttgtgaaa gcatctcttt aggtatctta ttcaataaag
     2641 tgatgtaatc agtattattt ggtgaatata agaatgggtt tttatcatat aatcgataca
     2701 acatctttaa ggcttttgca aaaccttctt tttgaggcat gttacctaat atttcatcta
     2761 ctgatgatat aagtctgttt tggattccat ttagtacttc aaaaaataac ttttcttttg
     2821 aatcaaagaa tgtgtagaat gaaccctttg caattcctga ttttgaacaa agttcatcaa
     2881 tattagtttt tttgtatcca tatttagacc agttgatttc acattctgaa attagtcttt
     2941 ttcttatatt atctttttct atttctgtaa agcttttagc catatttatc aatcctctct
     3001 gtatatacgt gttatctatg acttaaaaaa atttttaagt catagatata tagtattgtt
     3061 tattcataaa tttgtcaagt gatttatatt atgagacttg tattaaaatt tgatttgaat
     3121 aagtttcaag cgagtatgtt attaaaatag aaaaatgtag aaaaaacgtt aactctaaaa
     3181 aatttttcta ggtaaacgtt tataagaaaa agaaatgttt acataacgaa taaatggcta
     3241 taatttaaat acacaaagtt atatcagaga gaggaataaa aatatgttta atgaacttag
     3301 aacaaaagaa aatgtatata aaaatttaat aaatggaaaa tgggtagaat ctaattcaag
     3361 aaaaccaata gaaatttact caccaataga taattctctt gttgggaaag tacaatctat
     3421 gacaaaacat gaagtagatg aggctataaa aaatacaaaa gagagtataa aagtatgggc
     3481 tgatatgcct gtatataaga gagccaatat atttcataag gctgctgatt tattgttaga
     3541 aaatatagat gaaatagcta atatactggt acttgaaata gccaaagaca taaaatctgc
     3601 aagggcagaa gttgagagaa cagcagattt tttaagatac acagctgatg taggaaaaaa
     3661 tatggaaggt gaagctatta gtggagataa tttccctggt ggaacaagaa ataagatgtc
     3721 ttatgtttca agagtgccat taggtactgt tttagctata tctcctttta attatcctgt
     3781 aaatttatca atgtcaaaga ttgctccagc acttattggt ggaaatgcag tcgtattaaa
     3841 acctgctaca caaggggcta ttagcgcact tcatgtggta gaaattatga gaaaagctgg
     3901 tataccagat ggagtactta atactattac aggtaggggt agtgagatag gggattatgt
     3961 tgtaacgcat aaaggaatta attttattaa ctttactggt agtactgaag ttggtcagca
     4021 tatatctaaa atatctggaa tggtgccatt attattggaa cttggtggaa aagatgcggc
     4081 tatagtatta gaagatgcag acttagattt tgcagctaag aatatagtat ctggagcgta
     4141 ttcatattca ggacaaagat gtacggcagt aaagagaatt ttagtacaag agagtgtagc
     4201 ggataaatta gtcggaaaaa taaagccatt ggtagagaag ttgacaattg gaaatccaat
     4261 ggatgaagta gttatcactc ctcttataga taataaagca actgattttg ttcaagggtt
     4321 agttgatgat gcattacata aaggagcaaa gttaataaca ggcaatgtta gaaaaaataa
     4381 tttattctat ccaacattgt tagataatgt aaatgttgat atgaaaattg cttgggaaga
     4441 accatttggt ccagtacttc caattataag agttaaagat ataaatcaag caattgaaat
     4501 tgctaaccaa tcagagtatg ggcttcaatc atcagtattt acaagtgata ttgatagggc
     4561 attttatata gctgataaac ttgaagttgg tactgttcaa ataaataata agactgaaag
     4621 aggtccagac cacttcccat ttttaggtgt aaaggcatct ggtatgggaa ctcaaggtgt
     4681 taagtattca attgaggcta tgactagacc taaggcagta gtggtaaatg ttagagagtt
     4741 gtaataaata gagataaaga atagtagaga taaagaataa tagaaataaa aaataaagga
     4801 gttgtcttaa atttgatttt ttgtaattga attttaagtt caactccttt ttgaattttt
     4861 atatgctatt tcttagtaat tattttttaa taaatatctt aaactttgag attgatgaaa
     4921 tttgaacatg ataaatatct taaatttata gtcttgtttt ctaatgatga agcctatttc
     4981 tacttaaata gagcaatttc taaaatgaac atttttatct cgagtaccat atacaatgaa
     5041 aaaaattaac cttgacaagt gaccgatggt cgctgtatga taaataaaaa agaaacgacc
     5101 agtggtcgct aaaaggaggt atcgccctat gccaaaagga attgtgctta cacccgaaca
     5161 acaagcagaa cgccgagaaa aaatagttgc tgttgctttg agtctcatgg ctgaaaatgg
     5221 attccaaaaa acatcaatga gagaaattgc agttttagcc aacatgggaa agtcaagtct
     5281 atacgatttt ttcaaaacga aagacgagat tgttgtatat gcagttgaga aagaaataga
     5341 aaaaactatc caacaagttc ataggattat tgatggtgaa ccttcgccac aacagtgcct
     5401 tagaaaaatc atgcttaatc atcttagatt tccagagcag tatagaactg tactgatgtg
     5461 gttaaataca gaatctgatt atttagaaga agagtatcga aagagactta aagatgctcg
     5521 ttatgcatat caagatatta taaaatcggt aattgataat ggtgttacca caggtatctt
     5581 tcgcaaaacg gataccgctc ttgtgacacg tctgttaatt aactccatgt tatcaatcag
     5641 ctacacgtcc cgaccatcag atagttcgga aaaaatgttg gatgaaacaa tgaatatatt
     5701 tctgcacgga attatgaatg atggaggtga atgattatga tttattatgt cttaccacta
     5761 gaacataagc aagcaaccat tgaaattgtt ggcggaaaag ggatgtcttt atcaaaacta
     5821 ttgacagcag gcatctctgt gccagacggt tttcatgtta cgactgcttc ataccagatt
     5881 tttgttgaga cgaaccatat tcagtctcga attaataaac tactagatgg tattgattcc
     5941 aataatacca gccaacttga agatatatct aagaaaatag gggtgctttt ccataatgga
     6001 gaaatgccac aggaagtgtc ggacgcaatt aaaacagctt atgctggatt agggaacata
     6061 tcggtagctg tccgttcatc tgcgactgct gaagatttac ctgacgcttc ttttgcaggt
     6121 cagcaggaaa cgtatcttaa catacaaggt gaggatgaag tcattgacgc tgtaaagaga
     6181 tgctgggctt ccttatggac agctcgtgct attgcttatc gtgtgaagaa taatataaag
     6241 cacgagattg tcgcacttgc tgttgtagta caaaagcttg cattttccga ttcatctggt
     6301 attatgttta cgttaaaccc aatcaatggt agacgtagcg aaatgattat taacgctgca
     6361 tgggggcttg gtgaagcggt ggtttctagc ttagttacac ccgatacaat cgtagtggat
     6421 aaggactccg aacgaattat atcgtatgaa gtggcaaata aagagattat gacggttcgt
     6481 acttcagaag gaacagaaga aaccatggtt cctgaaaggt tgagaaaaaa atatgctctt
     6541 actcgtaatc aggtaatgca actgatacag cttggaaaga aaattgagaa gtattatcaa
     6601 atgcctatgg atgtagagtg ggcactggaa aaagataaat tgtacattgt tcaggctcgt
     6661 ccaattactg ttctgccacc ggaatgggta ttgccagaac aggatgttgt atacacaaag
     6721 ggaagtttgg cagaacactt accaaatcct gttacacctt tgttcgccac gcttggtctt
     6781 gaaatcgtaa accgtgcatc ggagctttta tggatagata tgtttggtaa aagtgcgaaa
     6841 aaactactgc caaaaaacgg ggcatacacg gttattaatg gatatgttta tctttctgct
     6901 aaatcaaagc cacttttgat tgctatcaaa tccctttccc cacgctcttt acgtaggaca
     6961 cttatgaaca gcgttgcacg ctgggaaacg gcacgaaaag gatatgagga tgctattaaa
     7021 caatgggaag aaaagcccct gcatacgatg aacgcccatc aaattatgga gggtattcag
     7081 acaatatttt atgccgcatg tatttatttc acaaggattc agcttacgtt gcctgctgct
     7141 tctatcagtg aaacattatt tacaaaatta tttcaaggag cagttcgccg tgctggtata
     7201 acggatactt ccgttttcct gcttgggttt gatacaattg ctctgcaatc ggaaaaggag
     7261 ttgtggggta tttcagaatg ggtaaagcaa aataacagca tcaaccttta tctgcaaaac
     7321 agttctgcaa taaagatagc agaagatttc atgtcttcag ttgtgcctgt tggagtttca
     7381 caggaagtat ggatagaatg gaaaaatcga atcaataagt actttaaaga gtttggtcgt
     7441 accgcttatg aatttgattt tgcgtattcc acaccacagg agacacttac accaactttt
     7501 gaatccgtaa aaacatttgt ggaaggaaaa ggggaaagtc cttttttacg ccagaccacg
     7561 tttgaaaagc gtagaaaaca tgcagaagaa gagattttac aacacatagg tggattgcgt
     7621 aaaaaactat tttttaagtt gctccattgg gcgcaggaaa cctctcctat gcgcgaaaat
     7681 gctatttatt tgatgggaat gggacatcct ctgattcgct gtatgtttca ggaaatctca
     7741 gaacgattca tacaaggagg agctatttca catttagatg atatttattg gctcacaaaa
     7801 tcggaattgg aggcacttat agagcagttg gataaaaata gatctctatc tgatatgaac
     7861 gggttaatac ctgttcgaaa agctgaactt aaaaaatata tgggttatgt gtcaccatcc
     7921 aagttgccag agaaaaacaa aaaaactata tcacaagcac tacagaagca gaaagatggg
     7981 aaaattgtgt tgacgggcat tggtaccagt actggtgttg tgacggctcc agcttgtgta
     8041 ctgaatagtc cagcagattt tgaaagcttt caacctggaa gtgttttggt tgcagttacc
     8101 acaactccag catggacacc attattttca tctgcaagtg cgattgtaac ggacattggt
     8161 ggtcctctta gccattcaag cattgtcgca cgtgaatatg gcatcccagc cgttatggct
     8221 acacacattg ccacacgaac tattaagagc ggacaaatga taacggtgga cgggttggca
     8281 ggtacggtga cgtttgatga ataaaaaggc tattttagct ttgaagcttt caacctttgt
     8341 gattgcgtta gcggaagtag taatacttaa agttttgaga tttaatcata ctactaatac
     8401 ccaccaaaca gcggtaagaa aaaccatagc ttggtgggga ttttcttatg caaaaaaatg
     8461 agaatgtatc aaaataaaaa tttgattaga aaaaatcaat tttgagacaa ttcctttatt
     8521 tgttgacaca ataatatcac ttataataag caattttata ttgttaattg gaggagtttt
     8581 gaaacataag tttttcaaaa tcttttattg gtaaaggtct agagaatacg tatccttgta
     8641 acatgtcaca gtttatttct tgcaagaaat ctttttgaaa actggtctct attccctcag
     8701 ctacagtggt catttttaac tttttagcca gttcgattat agatacaatt atatctcttt
     8761 cacgttgaga atctaaatgt tctgattgaa aaaatgctct atctaatttt aatgtatcta
     8821 cataaatatc cttaagcata ttgattgaag aatagccact tccaaaatca tcaatagagc
     8881 atttaaagcc atttttatga atttcattta ttatttcact aaatactttg acatcttcaa
     8941 atacaattgt ttctgtaatt tcaatttcta taaggtgaga aggaatacca tattttttag
     9001 ctattttatc atatgtcttg atgaaattat tggatgaaaa atgataacga gacatattta
     9061 ctgaaatatt tataggattt aatccatcat caatccattt ttttaaagtc ttgcaaactt
     9121 cctcaaaaac atataaatcc aactgtataa taaatccatt tttctcaaac aatggaataa
     9181 attcgtttgg aggaattatt ccttcaacag gatgaatcca tcttactaaa gcttctgcac
     9241 cacatatggt ttgggtagat aactccaact tgggttgcaa ataaacttga aattctcttt
     9301 tttcaagtgc ttcatacatt ctattttcta tgctttgttc tagaataagt ttttccttat
     9361 cttttttact atagaaatta caaatacata aaccgtgatt ttctacgttt ttaattgatt
     9421 tagaagctac atttgcacta tcttggatat ttgttatatc taaagaagta tcataaatta
     9481 catatgcacc agcagtaaaa taaagtatat atttataatc tgcgttattg ttgaatctat
     9541 taatatgtgt aaccattgag ttcagtctag aagttatatc ttctggttta tcttttaaaa
     9601 gaagaatgaa tttatcagca aagattctgg tcaccaattc accctcatgt aagtgtttac
     9661 ttatacagtt gtagatatat tttaaaagat tatctccagc tatatttcct gctaaatcat
     9721 ttattagttt aaattttttc atattcagcc atattagagt gtatgtaccc gatggtgatt
     9781 tactaataat ttcttttgct attatgttaa actttgtacg atttatacca ccagtaactg
     9841 agtcaataaa agctaatttc tttaattctt tattgttttt ttcaataatc tcgttttgtt
     9901 ttactaattt cgtgacatct gaatatatta aaaggtatat aggcttatta tcttcccaat
     9961 ctatacattc agctacaact tgcatatgaa caatatcccc attgatattt tgtggagaaa
    10021 cagaaaaaca aacgtatttc ccagctttaa aatcactttg atatttgtac acagcactaa
    10081 gataactttc taaatttata tcatctaata gaaaatttac attatctttg aacatgtcaa
    10141 agtatttttt attagcatca agaagttgga atccaaagtc agtaacctta aacttggcaa
    10201 tgagacctgg aaaattgttg taagttacag tttgttctat tttaatcatc atttgttctg
    10261 taatatcaat cataacagag tatgaaatag gatatccatt aacgacttct cctgttaaaa
    10321 atccaaataa ctttatccat aatttttttc cattacggtg tttcatacga cagacatagt
    10381 catatttttt tgctccggtg gagaatgtct tttctacata ctctacaagt tcattccaat
    10441 cctctggatt atccttgtaa aagaggtcgc attgattgtg aaatagagtt tcgtattctt
    10501 cttttgtata gccgaacatt tcataataac ggtcattaga ccaaacaaca gtaaagtgtt
    10561 catctaatag gtgtttacta acacttacat ccagtgcatt cattaataaa ctgtattcaa
    10621 tatttgcaaa atctatatat tttgacattc ttagtactcc cttataacat atattttgta
    10681 gaatatctga actagctagt ttaaagtaga tttattttta agcttatttt catacatcaa
    10741 agaatctgcc tctgctatta aagtctcaat atcggatatt ccatcccact caactaatcc
    10801 atagcttatg gaaagagcat aaggatactc gtcggtatta cttaattttg ataattcata
    10861 atttatatca atcattattt tttctgcttc tatacaactt gtattctgta aaaggaataa
    10921 aaattcatct ccaccatatc taaacagtaa ggaattttct acatttataa gtttttttat
    10981 tatttgtgca gtagtggata tgtatttatc accaaattgg tgtccaaatt tatcatttac
    11041 aggttttaag ctgtttaaat cagtaaaaca taaggtaaat ttgtagtgat ttgatgtcca
    11101 ctctgatagt ttttgattta caaaaggtct attgtagaag cctgtcagta aatctgtata
    11161 tacaatatca ttcaatttat taatttcata ttctgtcttt atactttcta catattctat
    11221 ttgattttta atatcataac cttttatttg agtaattcta aagctatatc tttctgcaat
    11281 atagttacct accaaaacta ttatggcaga cttgattcca tataatttta aaatcaatac
    11341 atttccaaca gtatagtata ttgacaaact caaacataga tacaagtaca ttccaaacat
    11401 aagactcaat aaaaaaatta agtttttttt gtcctctaaa actgcctcaa agttaatctt
    11461 attattttta tgatttttat aactgaaata taatcctgta actataaaac taagcaatat
    11521 agaaaatact ctgtaaatat ttgtaataga attactatca aataaatatg ctggcatatt
    11581 taacactatt gacattaaag acctaaaaaa tatatttatt gctaaaccaa tgatggagca
    11641 gtttaacgaa tagaaaaggt tttttattgt ggaaagtttg taagttactt taatttgcat
    11701 aaataaaatt aacataaaaa ttgtccaatt tacaactaga tgtaacctta aataagtaca
    11761 aataataaaa attaaataat taaaaagaga gtatacaatg tatactttgt atttattttt
    11821 agagttatta atcattatgt ctaaatattt aaaaaaatta taatgataga gataataaga
    11881 taaaaaacat agtaagatta caagtaagct catgtaaata ttcattttag ttattgttcc
    11941 ttttatggtt attttgttta ttagtgtaca ttctaatatc agcaatatta ataatttctt
    12001 ctacagtcat tttgctttcg ctaggaactt ccacaatacc atagctaaaa gatatcttat
    12061 tatccataat tttacttatt ttctctataa attcttttgt agtgttttca ttagatttag
    12121 gcataataac tataaactca tctcctccta ttctagcaaa tatgtcatct attcctaagt
    12181 tttcaataac tatacttgag aagttaataa tatagttatc cccagataaa tgtccatatg
    12241 tatcatttat cgtctttaat ttatccaaat cgatatatat caatgaaaat aataaactat
    12301 tatcaatgta atgctgtatt ttttcaaaag caaattttcg attatatgct gatgttaaat
    12361 catcaatgtt agcttttttc tttaataaat tgacttttag taattgttct tgctttatca
    12421 tttttagtct attatgttct ttttctaaat aaatattttt ttcaatggaa tatatatgat
    12481 tgatgaatat aattagttgt aagattaata atatgttact tcccaatata aagtaggaag
    12541 ttagagaact tggtggagca aatgtaattt gtatactatc aaataatata tatcctaaag
    12601 tgaaccagat aaacttcaaa aatagactga actctatttg attttgagtt attatataaa
    12661 tatgttcttt tattttatcc atatagtacc ataagataaa atctaaaata gaagctacta
    12721 aaatactaat taacttatag gacatattta cagatactat atctggactt gtatttttaa
    12781 caagtgacat tgtactcaca gtaataaggt gtaaaataca aaaacttatt actgctatat
    12841 tgggaataaa atggttgtta gtagaacttt ttctgcttaa gagcaattta tgatttagta
    12901 tgtaaaacaa tatataatat agtggtaatg gtaattttgt gaatattatt atcaagaagg
    12961 ttaatggttt tattaaaaaa aaatatttgt aatgcttatg tatagttata tttttgtctc
    13021 caaaagatgc tattttgcat atgtaaatgt tacatagttc gattattaat atagtagtta
    13081 tgattaaaaa tatatggtat ttcatagtac atcctctttt aaagtgaaat tgtatttact
    13141 taaaatagat tagattttat atattctatt ttacagtttt tatttaggta catgcaaaga
    13201 gcttattatg gataccttct tttttattgc atgtgtctgt gttagataaa atttaaaaag
    13261 tattctatct tacatataca gtatgtatga taactatgtg tccaagtata gtaattatat
    13321 atagtatgta tgataactat gtgtccaagt atagtaatta catatagcaa atataatagt
    13381 tatgtctcca attataacaa ttacatatag tatgtatgat aactatgtgt ccaagtatag
    13441 tataattaca tatagtaaat ataatagtta tgtgtctaag tatagtaatt acatatagca
    13501 tgtatgataa ctatgtgtct aagtatagta attatatata gcaagtataa tagtcatgtg
    13561 tataaatata gtaattatat atagcaagta tcatagttat gtatatcaaa tatagtaatt
    13621 acatatagca agtataatag ttatgtgtat taaatatagt aattacatat agcaagtatc
    13681 atataattta ttatgtaaaa caagtatagc tattctatta acgaatatac ataatatcaa
    13741 aaacatatat atgttttgct ggaattttga agtacttaaa attattccaa ttaatatact
    13801 acaatggtat gttaattttc tgtagtataa ctgaaatatg ctattttact gcattacagt
    13861 attataaaac ttagaccata tatataaatg gtttatatta attatagatt atgaggaaaa
    13921 aataagcaac tattttgccc tattaaataa aagtaaatag accttacaaa aaacaataaa
    13981 tctaatttat cgaagaggaa aaatatgaag agaaaaccac aatatttaat tatttttgag
    14041 catataattc aacaaattta tactggtaaa attactattg gagataggct tgattctata
    14101 aaaagcatgt cagaagaata tatggtatca aaaaatacta taaaaactgt aataaaaatg
    14161 ctctcagaaa agggatttat agagacaaaa gttggttcta gaccagtttt aatccagagt
    14221 acgagtaaac aatcaataga atctgactta ttatatgaga agattttaca aatatcagaa
    14281 atatataaga tttttagttt aatatttcct tctattgcta tgtacaatgt taagagattt
    14341 acatctaaag actttgaaga attaaatgag attatcgatt gtatggaaaa aaatagttct
    14401 aattatttaa tctttagaga gcagaggcta aaatatataa ataaactaat aagtaaaacg
    14461 aataatttcc tgatttatca tttttgtgag attattcagc atgatatgat tatttcacag
    14521 ttggcctata gtttcgataa cttggataat tgttgtatag acttttttcg taataaatac
    14581 atagagcaga taagaaaaac ttacttatat ggattggatg gaaatttaat tgaattaaaa
    14641 aaactattaa catatatcta taaagcttgt agagaatcca tacttaaaat ggtagaaaat
    14701 cgtgctccac aaagtagaga tacaagtagt tttaattctg taacattgga aaaatgttat
    14761 ttatatgact taattgtttc agatgtaatt tgtcagattt ttgaaggaaa tattaaaata
    14821 ggagattgtc taccatcaat tacttcgata attaatagat ataatgtatc tcttccaaca
    14881 gttagaagtg cctataatga gttaaatgaa ttgggaattg ctaaaacaat taatggcaag
    14941 ggaacttata taacactttt ttctgaatat gatgatgatt atataaatac agaagaagga
    15001 gctaaaaggt tgcgtttgct tttggatgct atggagtttg ttacaattac tcttgaggat
    15061 gtagtatttt tactaggaaa taaaatagat ttgatagctg tagataaaat agaaactcat
    15121 ttaaggtatt tacaaaataa ttgtaaagag tgtaaggttt atccagattt tgttttactt
    15181 tgtaagatag tagattatac acaaatttat atattacagg aaacattttt gcatttaaaa
    15241 caatatatga tatttggaat ctatttagag agattttttc atgaagaata taatgaaatt
    15301 ttgaaaacac gttttgatgt atgttttgat atacttaggt atttaaaaaa aggagatgtt
    15361 gagagttttg cagaatcttt ttccaatatt tttaaggagt ctttacaata tttgaaaaag
    15421 gttatatctt cattaaatcg ctgatatgag attaaaatta ggtatattgt aaaaaatggt
    15481 aaatgctctg tggagaaaaa atcctttttt taattgaaaa agcccataaa gtaagcattt
    15541 ctgctgtgtg caatacaatc ctgtaaaggg tgaaatggtg ttgtcatgta gtctaagtga
    15601 acaagcaagg ttacaaacag taacatgaag ttatgaaatt aaataagctg tttgacattt
    15661 ttacgtgctc atattagaat taaaaaacta agttatgtat tttttctact taagtgaaga
    15721 aggcatagac caatcaaaaa tatggaggat tataaaatgg gaaaagatag atttgaaaaa
    15781 gtatattcac aaggaaaaat aaatgtaatg gagatttggg tggataaaga aacaggtgtg
    15841 aattatgtat ttcactatta tggcaatgca gccggattta cgcctttact tgaccgagaa
    15901 ggaaaacctg ttatttctcc aattgtaagt aaataacgtt taaatataga aaaatgtaat
    15961 agatttttgg tttgtagggc tgttttaact ttctttattg attcctttat tggtataatg
    16021 catacttcct atttaaccat tcttaatagt agcaagtaat gtttcgtgtt atatactaag
    16081 cgattttatt gatttttgtc atcataaaaa ttgtaaaatt tgcttgtaat ggctacccca
    16141 attcacaaat aaggaatata cctgtggtct tttaagccaa gattcgtaaa gtttgtcctg
    16201 tttcttgtgg tttcaaggag agggtatacc accttatgat acttgaaatg gttattcatt
    16261 cagtaaaaaa taataataaa gtttctatgc gattttaaat aaaacaagtt aagtaatgga
    16321 tattttctaa gtataataat tgaaaatatc cattattgta tttagaatat attcaaaaca
    16381 agatgtgttt catattgtag aataatctta ttatatgtaa agttaagtta ttttataggt
    16441 atttttatcc agttatgcat tttcattgac cagttacaca ggaaatattg aatattgaga
    16501 aaattggtac tactatatat ataaataaaa atatatataa aaggaggttt ttatatgagt
    16561 ctttttgttg gtggatggtc aaaatttagg gttatcgaag aagaagattt aaaagtattt
    16621 aatgaagctg taggaatgtt aaaaggggtt gattatgaac cacttattgt tgctactcaa
    16681 gttgtagcag gaacaaattt taaatttatt tgcaatgcta cttcagttac aaatcctcca
    16741 cagaattatt tagcagagat aattgttttt aaacctcttc cttgtgatgg acataatgca
    16801 acaattacaa gtattaatat aatcaagtaa tattaatagg aggtggaaga tgagactaaa
    16861 tgtaactaaa caaaatacta tagaagttga gaatagtaga aaagaagatg tttattttgt
    16921 aatagttgat aatgtttttt ttgaacgtca aggtgatgta tttaatagta tcacgaataa
    16981 taatttaaat aattatgatg taaaaatatt accaactgat aatgtttttt tagaattaaa
    17041 aataacatct actaaagatt tagaacctgt ttttattgta gttgaagata gacaatacaa
    17101 tcatggtagt ataatgttta agccagtatt ggtataaaat ttaagcaaat aaaagtgaac
    17161 tattcaagta ggtataggtg aaatatttaa actaaaggaa acctattaaa ccagtatagt
    17221 tgaacttatt taagcaaata aagataacct atttaaacca gtatagttga attatttaag
    17281 caaataaaaa taaaatacaa gctggtatag gtgtaaaact taagcagtag tataggaatg
    17341 tttaaattaa tggtacaaat ataattaaaa gtatatatgt aataaccata ggaaaataat
    17401 ggacatataa tataaaaaac aactgtatta cataaaatac aataaaataa gtgcattaaa
    17461 taaaataact gtgttggtaa aaattatatt ctgcaacaca gttattttaa taaacaaaga
    17521 aaaataatta tttagtgaaa atgcaaattt attaaaaata aaaaataaat ttaaatatag
    17581 tctaaaaatt ttacaatcta cttaagggat taaactttat ttaaataaaa tatataatca
    17641 agggggaaaa taaatgccag aaaataacaa tcaactttta gaagaaataa cactcttatt
    17701 aaataaagga aaagcagcaa taaataaggc tgaaaatcaa aatttaattc taattcttgg
    17761 tgggacagga tgtggcaaaa gtactgcaat taactatctt gcaggatgtg taatgaggga
    17821 agtagaagat gaagatactg gtatatcata tattgagtgc ttagaccctg tggcagagat
    17881 tggttcagga gctgtttctc aaactttata tccaaacatt gtaaatatga gaaaaccaat
    17941 agaaggaatg gatgcaaaat tatgtgatac tgcaggtttt agcgacaata ggggagtagc
    18001 ccatgatatt agtgcagcag tttcattagg taaagtattt aaaaattctg catctatatg
    18061 tgcagtaatt gtattgatac cagaagcaga tatattagat tctagactta caaaagtact
    18121 agacctgttc caacagttaa atatgtgtct tcataaagaa aattttaaag acagtataac
    18181 ttttttcatt tcaaaaagtt tcttggggag aacagaaaaa caaatatata atgttataaa
    18241 aagaaaatat gaacaattat gcgaaaattt agatagtaat atagaggaaa aagcttggtt
    18301 atttgaagaa ttactaaaag ataatggaaa aaatattcat ctttgtaagc cacttgcatc
    18361 agatgagagg gaaggtttat taaagcaagt actagaatta gatgaagtta aagataagac
    18421 atcagcattc cagtatccaa ttagtgcaga tgcaagatta gttttggaga cgttgataaa
    18481 agaaattcaa aacaatatcg tatctagctt agaagattat gttaaggcgt atacagaaga
    18541 ttttatcagt attattggaa gtataacaat gattcaagaa atagcaaaat atgatgaggt
    18601 tgtaaaagta tttaaaaact ggttggaaag tacaacaagt cttactatag acagatttat
    18661 caacgtatta gccaaacaaa gtggcttatt cttacaagat agttgcatga aattacaaaa
    18721 taagataaaa ggaaacttag atataattga tgctttaagt aaatatacag aacatggaac
    18781 tggggaaatt ttattacctt ctgtacatat ggaatttatt atagaaaagg caagtaagaa
    18841 gataatggaa caaaacttca ttataataag aaagaattta gaaaaagttt tagtttctta
    18901 tgaagtgcaa aaacaggtat ttacattacc aaatagagaa aaattaatgg aagttgcaaa
    18961 taaaaaggat tatgtatata gtgtaagtga ctttaattca attttgacaa atgttacagt
    19021 tacacaaaat gaagagttag aagacagtat aacaagatta agtgtatatg gagaagaagg
    19081 taatgagata attagagaat ttattggtaa atacatttta aatccattta tagtaaaaac
    19141 aattaatatt aatgataaga aaggcattga aataagagca ttaattccaa acttagtaat
    19201 tagtagtgta ttggaatatc tcaaaaatag aaaagtagat gacaatgttt ctacatttat
    19261 gttctttgct aaagatacta tttatatgga tgataacctt ggaatggact ttgcatcaga
    19321 gaaaaatatt attcttgctt gtaacaatct tgatgtaggt agtaatgtaa aaattaatgt
    19381 aagtggaaag agcggagaac ttgtagatag ttctatgtat ggtaaaaatg gtgaagatgg
    19441 tagagattca ggtaatattt acttatatat taaagaagaa attagaaatt atcgtttaac
    19501 actgattgca aatggaggaa atggttctaa aggaatagat gggaaaaatg gtgaaacagg
    19561 agcttctgga aggaatggag ctgatgcagg gtatacaagt tatttccacg gacagatgtt
    19621 taaagggtat tttgtaaaag gtacaagtgg taaaaaaggt gaaaaaggtg gagctggtgg
    19681 aaatgctgga agaggtggaa atcctggtaa taaaggtaat atttacttca tttcagattc
    19741 agaggaacaa aaaaatagaa taattaatca ttgtgatata acagtgaaag atggagaagc
    19801 aggtccaaat ggtagaccag gtttaggtgg atatggagga agtggtggtt atcgcggata
    19861 taatacactt tcattttctc caagtggtat agataagact gtgtactata caggatattt
    19921 tacaagttgg gattataagt tagatggtat aactaaatat catatagaat ttagtggcgg
    19981 aagttggaat agaggtaaaa caggaccagc aagagaatac gctgaatatg gagatagagg
    20041 agacaacgga ggatttaatg ctccaaacac taaattatca acagctgttt caaaggtaga
    20101 agagaatatt attaaagaaa aatatcaagg atattgttct gaaatataga aagggatgga
    20161 tgtatggcaa ataaacttta tagtgaaatc ataaaccttt tggaagaagg aagagacgag
    20221 tttagaaaat atgacttaaa agaaaaatca attttattat tcttaggtgc ttcaggcgtt
    20281 ggaaagagta cttgtatcaa ttatttaaaa ggctgtgtaa tggaagaaaa aacggatgaa
    20341 gagactggac aaatatatat aactgcaaaa gattcagctg tcgaaattgg aaatggtgta
    20401 tattccaaga ctttatgtcc agaagttgta gatattgcaa atagagattt ttcattatgt
    20461 gattgcccag gtttttttga taaccgtgga gcagagtata tgattgctgg tgctatgctt
    20521 gtaagggaaa ctatttctac atcttcaaag gtgaaaggga tggtagttat tttagactat
    20581 caatccctac tggactctag acatacatta ttaatagaaa taggcaaaaa tttgaaagat
    20641 atgcttggtg attatgcaaa atataaagaa aatttcttct ttcttatcaa taaaatacct
    20701 aaagcactaa tcggtaatgt aacagaagaa aaaataagaa atatattaaa aaaagcaaaa
    20761 gaagacctag atagtcaaga gaaagattac gacggttata aggaattagt gtacttatta
    20821 aatgtagcat tatcagatgg tgtatctatt aaattatgta atccactaga taaacagcca
    20881 gaaaagttgt tggatgaaat gaaaaatcta cctgaaacct ttgaaaaaac agatatggga
    20941 ttgatgctta ctgcttcttg taaaatagaa atacaaaaag ccgttgatgt aacctataaa
    21001 gacctagaaa aacttttagt tccttatata gaaagtgtca aaaaaggtat ggaagattct
    21061 atttccattt tatcttctat aaatagtcta aaggataaaa agattcaatt agacaagatg
    21121 tatacagatt tatgtagtag aactaataat atgaaagagc ttatggagtt tgtacaagaa
    21181 ctttcatatg gtgatggcgt acagaaagtg aatatattag aagaaacacg tcttctttta
    21241 gagttaaata gttattgtag tgagactcat attgttggtt taagtgagtt gttaaaatca
    21301 ttactaaaaa acactttaga agatacatta agggatatta aatatcagat aatagaagaa
    21361 gaaactaagg aagaagaaag caaaaagctt gaagatgcgg aatataggat agagaagatt
    21421 caaagttgta catatataaa atcatatgag aaagaaaatg tactttctat atatattcct
    21481 atacctaaag tgaatttatc ggatttgact aaaataatat ctggctatca acttaatatt
    21541 ttgtcatcta tatatatgta tggagattgt gagctaaaaa tagattgtaa tatggagaaa
    21601 gaagtcttct caggtaagaa tattgtacta cttacagaaa aagtatttat accagaaggc
    21661 acttattcaa tcaacgtatc tggtaaggat gcagaaaaat tagttggaga agattctatt
    21721 tacaacggta tgaatggaag aaatggcaat aatgcaggga cttcaggtga tattttatta
    21781 tcttttaagg agatagaaaa aagaggagaa ctcaaacttt atgcagacgg cggacaaggt
    21841 ggaaatggag aagatggaaa aataggagct acaggattaa ctggagaaat gggggcaaat
    21901 gctaaaaggg attctaaaag agagtcgaaa gcatttgttg aaaattatga atcaagcttt
    21961 tttatgataa aagcagaaaa aggaaaacaa ggtggaactg gaggtacagg aggtaatggt
    22021 ggaaatgcag gtattgggag caaatctggg cagatttatc tcattggaaa agcgattgta
    22081 ttaaaaagaa atataagcaa aaaagatgga agtcatggta tggcaggaaa aggaggttct
    22141 ggaggaaaag gaggctctgg aggtcttggc ggaattagag gtatatttga agtaacaaaa
    22201 acttttgata aatataatat aaactactat ttttttgata gatgcatatt agacggaggt
    22261 aaagaagtac cagatgatgg agagaaatta aaagcccttt atgaaaaaca taaggactgg
    22321 acaattagca aacgtgaaga gtcaggttca tatggtgaag atggaaaaga aggccgtttt
    22381 ggagatgcac taaaagaaag agaactgtta aaacaagaat attctttatc aatggataca
    22441 ttcttctatg agttccttga gagatttggc ttttctggaa gtaaggggta tagaaacctt
    22501 agattattgt gtgcagtagg aattttagaa gctgatattt tagaactttc acatatatat
    22561 gaaatttttc atgcagttga gaaagtagag atgcagttaa atagagaaat acttcagaag
    22621 tattctggtt ttgataagaa aaataaagca gtcatttctt tggatgatat gaatataaag
    22681 aaagaatttt ataaagaggc aaaggaaatt tatgaatacc ttataaataa attgatgcag
    22741 tctgatagaa tagaatcttt tataggagaa aaacagaatt ttagcgaatg gagtatgatt
    22801 ttatttgcac ttgaacaaaa ggtagccttt tatgttcaga tgatagaaga aaatgaaatt
    22861 ggaggaaatc gtgttacaga tattcgtagt ttgacagagt atttagataa gattttaata
    22921 gaactggaac aaggaaagaa ggatgagtta aaagaaggat atttcaatac atataaagaa
    22981 agtatcctaa ctcaaatcaa atatgggaat caacaaattg aagttttaaa aaaagaactt
    23041 gagaataaaa tgctttctgt taatgcaaaa atgaatctgt tgattcaaga gattataaag
    23101 ataaaagaaa atgaaaaaga ggacatagag aagctaaaag aaaagcagaa aaaacttaag
    23161 aaacaaatga aaaaacaatt aattctttca ggaatacaaa gtgcattggg cttactaaca
    23221 gcatttagtg gtgttggaca agcttgtcta ggagcatttg atagtgcaaa atctttgcag
    23281 gaaaatcttt cagctaaaga agaagaatgc aagtggacta acactatgtg tgatttttca
    23341 gattttaagt taaattttga tggtatagac tttgtagtta aaaaacagtt aaaagaagat
    23401 agaaaggaag ctttaggtaa aatgacttat aacttggcag ggattgatgg agtaacagaa
    23461 gttaaatttg accaaattaa agctatcatg gttaagtcta tagaggataa taactacgca
    23521 aacaaagcta atgatattca aatagcaaaa gatggtttaa atgaagggaa gtatgaggaa
    23581 atttactcaa aaatccagaa aaaagaaata ggtatactag aagctaatta taataataaa
    23641 caacaaaagg ataagttgga tgcactaaga aagagtaaag aggctattga aagacaacaa
    23701 aaaatatcta ctattaaaaa tgcattaagt attggtagtt ctgcttgttt tcttgttaat
    23761 acagcaatta gtggtgttaa taattgtctt aatgtaaagt ctagttttga aagcaaaata
    23821 gaggtagtta aagaggcaat taaagataaa gaaaataata ttaagaagtt agatgaatta
    23881 aatactcaaa tggcagagtt taaagataag acttttaaag aatctattat accatttgta
    23941 gaaaaatttg atgatacagt taaaaaccaa gatatttttg aaaatgagat aagtaaatta
    24001 aagatgaagg aagttttaac tgaattaagt acaaagtttg caagtttaaa aatggaaaat
    24061 tcagaggaca tttctataat cttcaaaaaa gtacaggata tgatggaaac tcagattatc
    24121 attactgata agattgataa gaaaaaagat aaccttgcaa ttggagaatt aatttatcaa
    24181 atatcagata gtggtaatca aattaatgaa tatgaaaaaa aacttattca ccaaatcgaa
    24241 ttaaatcaag ttagatattt gtgtcaggtt gaggcagcag cttttcaact atggacgttt
    24301 ccatttggaa gcaatatagt aaatatctta aatagatgcc atgtaggtat cacagaagat
    24361 gaaaatgagg ttgtaaatca agccaaggaa cagaatagac agattaaaga gtttcttgac
    24421 caagatgttt accaatggca aaaaacggat aaccatattt tgaaggtagc attaacaggt
    24481 agtttagatg ggaaatcacg tgtgggattt gaaaatacat attctctata tgattgtaat
    24541 gaagaagaag tgaaaaagct tttgacggga gatgaaatta gttttgaagt tccattaaat
    24601 caaaactttg atgccataaa atatgtaacc atgtatgcaa atatcccgaa ccttagacga
    24661 aatattttag aggatagttt aaaagtaaag gtgcatattc atttatatga tagtggatac
    24721 ttctctctat atgatgataa aaaagatgtc tattatcaat atgtatttcc acaaaactat
    24781 atagatatta cacatggtct tgatgttaaa tacaatgatt cagattcaat aaactcaata
    24841 tggtcttcag tagaagataa aggaattgaa aagttcaaaa atgcaggatt aaactctggt
    24901 agaagtccat attctatggc taagatttct ttgaaggcac attttaataa gaagctttct
    24961 tatgattcac ttacaaatga gcagaagaag aaactagaat catactttga taaacctagt
    25021 aagtatgcat ggagtgatag tatagatata ttgaagagtc tagggtatat tgatttgaag
    25081 ggaattttgt caaagagttg gtatgaaatc aagaaagata tagatagtga tgcggagatt
    25141 atgaagcatt tttctatatt acaagttgat ttattggaaa gaattattta tgagcagact
    25201 agtatactaa aggaaaatat acctaatttg acaattgaat tggtaggctg tggtatttat
    25261 gtagagtctg aatttaatta tctaaagcaa aatgatttaa aatgctatga agagagattg
    25321 gttgaaaata taattaatat ataagtttta aagaaattta caaaagggga tgtttcaaaa
    25381 tgaaaatttg aatcatctcc ttttttgtaa aaaatagaaa taagttaagt ttaaaatttg
    25441 taaagcatgt tttataattt tgattagaaa aaagctactt ttaagacagt ttctttttaa
    25501 aagatgaatt ataaattttt tatatacata tgttttatat aatagggatt aatgatagtt
    25561 ataagtattg gtgttttcca ataaatttgt catactgttt aaacttattc cgacagtaga
    25621 tgcgttgtgt aaaaaggcag atgtacttgg tctaacaaat ccacctacac caagtaaaat
    25681 cagacctaaa ttaaagccga taataaactt agaacttaaa tcaattctct tcataagatt
    25741 attactaatc tgctttaaaa caattaaatt attcaaatta tctgctgata tggtaatatc
    25801 tgaaatctcc cttgctattt cagcaccttc actcattgca attccaacat ctgattctga
    25861 aatagcagga gaatcattga ttccatcacc aatcataatt actttgcgac cttttagttt
    25921 ttcactttta acaaaattgg ctttgtcttc tggtaagact tctgaataat attcatcaac
    25981 accaacttta cttgctatag atttagctgt tttttcgcta tctccagtca tcatcacaat
    26041 cttttttatt ccatattctc ttaaatttga aattacatat tttgcttctt ttcgtaaagg
    26101 gtcatttatg caaataactg ctgataattt acccgatatt gccataaata ggtgcgaata
    26161 ctcatctgat agattgttgt acttatcctc ctcaccgtct gggatagtac acttctcatc
    26221 ttcaaataca aaatgtttgc tacctattac aaccttttta tcatcaacct ttgttgaaat
    26281 accatgagca ataatatatt ctacctctgt atgcatttct ttgtgactaa gcccttgttt
    26341 ttcagcttca taaactactg catttgctat ggagtgtggg aagtgttctt ctaagcaagc
    26401 tgcaagtctt aacatatcct ccttattata attttgaaat gtaattatat ctgaaacagt
    26461 tggttgagct tttgttaatg tgcctgtctt ctgaacctcc catgactaaa gtcacagggt
    26521 tcctgcttca tagaattttg catactaaaa tatgtcttac taattctcca caggctatcc
    26581 ccatagttcc tacggttctt acataagtta tttattttga attgttatta gtcttaatcc
    26641 ttcttttagt atattcatac ctgcatttat atctctatcg tgagtttcat tacaactagg
    26701 acaaatccat tctcttatat ttaagtcctt aacttcctcg tttttatacc cacatttatt
    26761 gcatatttgt gaacttgcaa agaatttacc tacatttaca atttgtctac cataccaatt
    26821 agccttgtat tctaattgac gaataaattc tgaccatgat acatctgaaa ttaagcgaga
    26881 tagtttatga tttttaatca tattctttac ttgtaaatct tctat
//
LOCUS       DAEPXF010000038        26686 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain HB27
            SAMN13376389-rid12479423.denovo.040, whole genome shotgun sequence.
ACCESSION   DAEPXF010000038 DAEPXF010000000
VERSION     DAEPXF010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13376389
            Sequence Read Archive: SRR10518432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 26686)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26686)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001069624.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001069624.1
            Genome Coverage       :: 315x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/15/2021 01:54:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,165
            CDSs (total)                      :: 4,076
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 89
            rRNAs                             :: 10, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 10 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 19 of 69
            Pseudo Genes (incomplete)         :: 44 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 10 of 69
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26686
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13376389-rid12479423.denovo.040"
                     /strain="HB27"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="China: Hebei"
                     /collection_date="2017"
     gene            complement(<1..554)
                     /locus_tag="KRL67_001402"
     CDS             complement(<1..554)
                     /locus_tag="KRL67_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HBH1522296.1"
                     /translation="MKICNDNFIKNFKNMINKCLDFIYPENISCIICDKSIKKTNTYS
                     ICKSCFKEMNFIQDGCMKCGKPIIRHSIEKEFIEECSYCFNKDFYFDKSISCIEYNDV
                     TKKIVLGLKYNQKTFMAKYIAQIMKEKLYLENIKFDYILFVPLHKKRLNKRGFNQAQK
                     IAFNLSKIVNIPLLDCISRKKYTR"
     gene            complement(557..2776)
                     /locus_tag="KRL67_001403"
     CDS             complement(557..2776)
                     /locus_tag="KRL67_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RecD-like DNA helicase"
                     /protein_id="HBH1522297.1"
                     /translation="MEKLEGMISEIVFKNEDNGYTIAHLVNENDEIVVVGCMPTLAIG
                     ESIEVEGKWVNHKIYGTQFEVNSFMPVTPSSLEGIYVYLSSGMIHGIGEKMAKRIIDK
                     FGVDTLEVIQNSPEKLQEVEGIGSKKVKQIVKSYEEDRELRNIIIQLSPFGITPNYCL
                     KIYKKYKSSAIEVINKNPYQLAEDIRGIGFKVADSIANKIGIDKNSKDRICQGILYTL
                     NKSLSNGHTYLPEHVLIQDSEKLLELNGEIIKECVMMLVYNQKIHIEKVNNENLIYLM
                     PYYLAENGVCSQIVKLSQYEFEDLKIDIDNEINVLEEDKKIKLAEKQILAVKESVNSG
                     VLIITGGPGTGKTTTINAIIDIFENNGKSVTLAAPTGRAAKRMSETSNKEAKTIHRLL
                     EMGFSTDDDLTFFKDEEDPINSDVIIVDEVSMVDIILMYNLLRAIKLGTRVILVGDSD
                     QLPSVGAGNVLKDMINSNIINVVKLNEIFRQAQESMIIVNAHKINNGEPLYLNTKGKD
                     FFFIRKSTNEEILNEIIGLVNERLPKFYKVDKLKDIQVLSSMRKGELGVTNLNIELQK
                     YLNKKEKFKVEESFSKRLFRVGDKVMQVKNNYTKKWETEDQKESGEGIYNGDIGYVYH
                     IDKDKKTIYILFDQTKIVSYLYDELDEIDHSFCTTIHKSQGSEFPVVVLPIAWAPPML
                     LSRNLLYTAVTRAKKLVVLVGDVKYLEYMIKNNRVNQRYSNLGYKLNKFKQEGLLIE"
     gene            complement(3040..4137)
                     /locus_tag="KRL67_001404"
     CDS             complement(3040..4137)
                     /locus_tag="KRL67_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH1522298.1"
                     /translation="MSGKVNIKTVISFAGAYVATVIGSGFATGQEILQFFTFYGYAGI
                     IGGIISMVLFSWFGAEVIDKGRELKLKEPIKIYQVYCGKYLGTFFEWFGPLFLFGTLV
                     IMIAGAGATLSEYYGLNPYVGRIGMAIVSLITVSLGLTRLSKILGNIGPIIIIFTLLV
                     GAISLFSNIDALSNAGNMVDSLNIKTATSNGYFSGVLYTCYNVIIVITFLTGMGASAV
                     NKKDAVWGGIVGGVALMAAAIMMNLALLSDIGNIYTKEIPALYLADKISPIIGILFSV
                     VLLLGIYTTAVPLLWSVTNRFVEDDHPKFKIITIVVSILACIGGLLPFDKLVGTLYPY
                     TGYMGILILLCMLYRRITKTEGYKENKSEIS"
     gene            complement(4453..5646)
                     /locus_tag="KRL67_001405"
     CDS             complement(4453..5646)
                     /locus_tag="KRL67_001405"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HBH1522299.1"
                     /translation="MARHLFTSESVTEGHPDKICDQISDSILDALLEKDPQSRVACET
                     TVTTGLVLVAGEISTSAYVDIPKLVRETVREIGYTRAKYGFDCDTCAVITSIDEQSGD
                     IAMGVDEGLESKTGEEIEEEIEKVGAGDQGIMFGFACNETPELMPLPISLAHKLSRRL
                     TEVRKTGLVDYLRPDGKTQVTVEYEGSKAVRVHTVLISAQHCETVSNDKIREDLINHV
                     IKEVIPAELLDEDTKIYINPTGRFVIGGPQGDTGLTGRKIIIDTYGGYSRHGGGAFSG
                     KDPTKVDRSAAYAARYVAKNIVAAGLADKCEIELAYAIGIARPLSIFIDTFGTGKVSE
                     EKLVELVNKHFDLRPGAIIRDLDLRKPLYKKVAAYGHFGRTDIDLPWERTDKVEQLRK
                     DALGE"
     regulatory      complement(5782..5885)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            6074..6583
                     /gene="yyaC"
                     /locus_tag="KRL67_001406"
     CDS             6074..6583
                     /gene="yyaC"
                     /locus_tag="KRL67_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore protease YyaC"
                     /protein_id="HBH1522300.1"
                     /translation="MYLAKVNYSDDDVIQMLSSVLKTIINENTIVVCIGTDRAIGDTL
                     GPLVGTILKNSNFKYPVYGTLDNPIHALNIYESLDTIKNTHIQGNFLAIDACLGSQSN
                     IGNIQIREGPILPGKGVGKKLPQIGNYSIVGIVDKIDENNKLSFNNIRLSFILDLAET
                     IALALLVST"
     gene            complement(6694..7122)
                     /gene="fabZ"
                     /locus_tag="KRL67_001407"
     CDS             complement(6694..7122)
                     /gene="fabZ"
                     /locus_tag="KRL67_001407"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HBH1522301.1"
                     /translation="MLNIDEIKKLIPHRYPFLLVDKITELEVGKRAVGIKNVTVNEPF
                     FQGHFPEYPLMPGVLIVEALAQVCGVAMMSVEENKGKLGVFAGIDKVRIKREVRPGDT
                     LTMEVEMTTLRRNIAKADAKAYVGEELVCKGELMFALVEK"
     gene            complement(7138..8157)
                     /locus_tag="KRL67_001408"
     CDS             complement(7138..8157)
                     /locus_tag="KRL67_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HBH1522302.1"
                     /translation="MAGADIGIDLGTANVLVYVSGKGIVLEEPSVVAIDNRTDSVLAV
                     GEEAKKMIGRTPGNIVAIRPLRDGVISDYDVTEKMLKSFIDKIVDKKGFGRFFMPRIM
                     VCVPTGVTEVEKRAVEDATRQAGAREVYIIEEPIAAAIGAGVDISQPNGNMIIDIGGG
                     TVDIAVISLGGAVVSESIKVGGDRFDDAIVKYMKKQHNLLIGERTAEKIKFEIGSAFK
                     REEEKYMKITGRNLITGLPSSITINSTEMLEALRECVEQIVVATHAVLEKTPPELAAD
                     IGDVGIIMTGGGSLLYGLDKIIEQRTGITVTIADEPLSCVAKGTGIALGSIDLLETGG
                     SFKRK"
     gene            complement(8354..8614)
                     /gene="spoIIID"
                     /locus_tag="KRL67_001409"
     CDS             complement(8354..8614)
                     /gene="spoIIID"
                     /locus_tag="KRL67_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation transcriptional regulator SpoIIID"
                     /protein_id="HBH1522303.1"
                     /translation="MRSHIEERAIVVAKYILEKNTTVRQTAKTFGVSKSTIHKDVTER
                     LKEINPSLAKEVKNVLDKNKSERHIRGGLATKLKYEREHKKM"
     gene            complement(8712..9380)
                     /locus_tag="KRL67_001410"
     CDS             complement(8712..9380)
                     /locus_tag="KRL67_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HBH1522304.1"
                     /translation="MKKKLLEKDGFYLSLFVCVCLLAVGGVWFTNNNVDKLASNKGIM
                     ENANKDSEEEIHLIEKDKKDAIPTATDSKQNLEKAKSKEENKSSTTKLNYIGDKVIRG
                     YSEKEPSYSKTLDVWETHKGVDISCTKGKEVKSLLNGIVVDVFNDEEYGQSVKIKSDN
                     NIVVVYSNLDKNVSVKKEQKVTEGQSLGTVGSTSQIESEEGIHVHLEAYSGEKSIDPM
                     SLIK"
     gene            complement(9446..10510)
                     /gene="spoIID"
                     /locus_tag="KRL67_001411"
     CDS             complement(9446..10510)
                     /gene="spoIID"
                     /locus_tag="KRL67_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein D"
                     /protein_id="HBH1522305.1"
                     /translation="MKNPLVVLLGFVTCSVLVPSLITLVSYKNVELTEKAESPVSINK
                     TIKKSDIEDKGNKEEKNTVNYETVNKKVPIINVYNHITGKTEKMDMENYLCGVLAGEM
                     SSEFDIEALKAQSVAARTYVVYKQEHGKSSKHKNAVVCTDYKHCQEYKSYDTLKKLNG
                     EEWIKNKYSKIQEAVRGTKGQIMTYNDKAILPLYFSTSSGKTENSEEVFSAKYPYLKS
                     VESPYDKYSPKFASTLKISNTDFVKSLRRAYSTIVIDVNNLSKQVSITKRSDAGTVEK
                     IKLGNKELTGKDIRTVFKLNSANFDIKFGEGYIDFVVKGYGHGVGMSQWGAEGMAEEG
                     YKYYDILSHYYTDTKIKDIY"
     gene            complement(10597..11850)
                     /gene="murA"
                     /locus_tag="KRL67_001412"
     CDS             complement(10597..11850)
                     /gene="murA"
                     /locus_tag="KRL67_001412"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HBH1522306.1"
                     /translation="MAKIIVKKSNPLKGSVKIDGAKNAVLPIIAATLLANGKSTLNGV
                     PNLRDVHVISDLLRHVGAEVEYKENTLTVDASNIKTCEAPYELVRKMRASFLVMGPLL
                     ARFNSTKISMPGGCAIGTRPIDLHLKGFKALGAKIEMDHGFVEAATEKLVGNKLYLDF
                     PSVGATENIMMAASLAEGTTIIENAAEEPEIVDLANFLNEMGADVKGAGTNTIKIKGV
                     KELKGAEHNVIPDRIEAATYMVAAAMTKGDITVENVLMEHLKPVVAKLREAGCEITEM
                     DNSVRVVGPKVLKPIDIKTLPHPGFPTDVQAQFMAMLTVANGTGVVIETVFENRFMHV
                     AEFNRMGANIKIDGRSAVVNGVDELHGAAVNATDLRAGAALILCGLIAEGETQIGEIY
                     HIQRGYVDIDKKITALGGQIEIVED"
     gene            complement(11871..12608)
                     /locus_tag="KRL67_001413"
     CDS             complement(11871..12608)
                     /locus_tag="KRL67_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YwmB family TATA-box binding protein"
                     /protein_id="HBH1522307.1"
                     /translation="MKRVKLAISFFILMIIGMTISYADTSINTRYNRLSKALEISEAN
                     FKFYNVKINSVINHNLSNKEFHNIFIEIINNLKLDKKGINWNQKGSGDKIQLCATIKG
                     SDKEISIKGINKNNKESYIVVDILDNKVYKHKEVIYSNVENVLNKYSSQVDVSACIVG
                     EYTKRLQNNKYDDILQKILYNMSAKQIDEVKDVNFLSITAYSKVLNDYELEYLGEKIN
                     LNIGIKYNEDDGRTLIYIATPIIKLDY"
     gene            complement(13079..14374)
                     /locus_tag="KRL67_001414"
     CDS             complement(13079..14374)
                     /locus_tag="KRL67_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase"
                     /protein_id="HBH1522308.1"
                     /translation="MTNDFKKEKKEDYFVNLKAISTEVLQEKVQDNAWVIVDTRLNDA
                     YNGWKLDGVKRGGHIKGAVDFSANWLSVYSDRKDEVLEQALKTKRIDLDKNIVLYDAN
                     GKDALVVADYLSKKGYKYLYKYDIKQWADNENLPMERYKNYQMIVPAFIIKDILDGKI
                     PETFEDSKNIKMIEASWGEESYTKGHIPTSVHVNTDIIEPPPTWMLDNDDNLIKFALD
                     YGLTKDDTVIVSSSTPMASYRLAVILRYIGVKDVRVLNGGTNSWLSAGYELEFTSNPK
                     HSCTNFGADIPVNSQLVVTTSELRQKLKEKNKFILVDNRTWDEHIGKVSGYTYYDKKG
                     RIPGALYGHSGSDSVSLEEYRNIDNTMRNKYEILEMWDKENIDVNKQLIFMCGSGWRA
                     AEVLTYANVIGVENTSLYSDGWMGWSLDNSNLIEVGEHK"
     gene            complement(14868..16700)
                     /gene="glmS"
                     /locus_tag="KRL67_001415"
     CDS             complement(14868..16700)
                     /gene="glmS"
                     /locus_tag="KRL67_001415"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003401499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HBH1522309.1"
                     /translation="MCGIVGYLGSRKAAEVIVEGLSKLEYRGYDSAGVAVNSSNEEEL
                     NIRKFKGRLSVLAEDLEKNPIDGNLGIGHTRWATHGEPSDVNSHPHFNQAKTIAVVHN
                     GIIENYMEIKEELISEGVKFESQTDTEVIAHLVDKYYEGNLLDAVYKTISKLRGAYAL
                     GVICKEHGNELVAVRKDSPLVVGVGEGENFIASDIPALLKYTRDVYFLENGEVVHLKD
                     ENVTVYDSNRNLVEKEVFHVTWDVEAASKGGYDYFMSKEIHEQPTGVRETLERRLDDN
                     GNIVLDSINISKEDLEKINKVYIVACGTAYNAGLLGKYAIEKFVNIPVITDIASEFRY
                     SDPFVDENSLVILVSQSGETADTLAVLRDSKAKGARILSITNVVGSSIARESDDVFYT
                     WAGPEVAVASTKAYTTQITSLYMIALDFAIKKGTITREFYDSMISKMKEIPSKIQEIL
                     DNEEYIKEVAKTVVSSEHAFYLGRGIDYSLAMEGSLKLKEISYIHAEAFAAGELKHGT
                     IALIEKGTPVIAIATQEKLFEKMVSNMEEVRARGAYVVAIAQSHNKDVEKAADKIIYI
                     PNSDDILSPILAVVPMQLLAYHVSVLRGCDVDKPRNLAKSVTVE"
     gene            complement(16967..18313)
                     /locus_tag="KRL67_001416"
     CDS             complement(16967..18313)
                     /locus_tag="KRL67_001416"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HBH1522310.1"
                     /translation="MRKYFGTDGVRGVANTELTCDLAYKLGRAGGFVLAQGDHRVKVV
                     VGKDTRISGDMLEASLIAGLMSVGCDVITVGIIPTPAVAYLTRKYGADCGVVISASHN
                     PVEYNGIKFFNKNGYKLDDEIELKIEEYIDDIDKIDCLPIGENVGRKLHEHCAQRDYV
                     DYLKSIISTDFKGLKVVLDCANGASYKVAPIVFDELGASVISINSSPDGNNINYKCGS
                     THPEQLQRAVLEHNADLGLAYDGDADRLIAVNEKGQIVDGDHIMILSALNLKKNNKLA
                     QDTLVVTVMSNIGLTIAAKENGINLSTTAVGDRYVLEDMVKNGYNLGGEQSGHMIFLD
                     YNTTGDGVLSSLILANIILQEKKPLSEIASIMSQYPQVLVNATIKNENKNKYMEYPEI
                     KTEIERIESILDRNGRVLIRPSGTEPLVRVMLEGKEEGQIKELATNLANLIQEKLS"
     gene            complement(18483..19040)
                     /locus_tag="KRL67_001417"
     CDS             complement(18483..19040)
                     /locus_tag="KRL67_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HBH1522311.1"
                     /translation="MSTARVICAGFGGQGVMSMGQLLTYAGMLEGKEVSWLPSYGPEM
                     RGGTANCAVTVSNEPVGSPLITDDATAAILLNIPAFEKFKDDVIPGGKIIVNSSLIKE
                     KVDRTDVDVYYIPANELAAELGNDKVANMIMLGAYLKVSDTVDIESVLEAFKKVFGPR
                     KEKFVPLNREALQKGMDATCSKATN"
     gene            complement(19041..19793)
                     /locus_tag="KRL67_001418"
     CDS             complement(19041..19793)
                     /locus_tag="KRL67_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate oxidoreductase"
                     /protein_id="HBH1522312.1"
                     /translation="MAVVFKKTEGLQDTQTHYCPGCTHGIIHRLVGEVLEELGVLGDA
                     VGVVPVGCSVLGYKYFNCDTQEAAHGRAPAAATGIKRVHPENTVFTYQGDGDLASIGT
                     AEIVHAAARGEKITTIFVNNTTYGMTGGQMAPTTLVGQRATTAQSGRNAETQGYPIRV
                     SEMLATLTGAVFVERVAVDTPAHVRQAKKAIKKAFQVQQAGLGFGIVEVLSTCPTNWG
                     LAPNDALQWLRDNMIPYYPLGNFKNVEVEEVK"
     gene            complement(19793..20872)
                     /locus_tag="KRL67_001419"
     CDS             complement(19793..20872)
                     /locus_tag="KRL67_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate dehydrogenase subunit
                     VorB"
                     /protein_id="HBH1522313.1"
                     /translation="MAKILMKGNEAFGKAAIEAGCKYFFGYPITPQSELPEYLSRELP
                     KIGGAFVQAESEVSAINMVYGGAGAGARVMTSSSSPGVALKQEGITYAVGAEVPCVVL
                     NVMRGGPGLGSIQPSQADYFMSTRGGGNGDYRTPVFAPATVQEAVDMIMEAFDVADYY
                     RSPVMVVADGMIGQMMEPVEFRAPEKKRELPPKDWATVGTKGKREPNVINSLYLEPEV
                     LEDHCWHLQEKFNAMEKNEVQYEMYKTEDAEFVFAAYGTTSRVVKSAIDILREEGIKA
                     GLIRPKVLWPFPFEAFNQIPNARNILTVEMSMGQMVEDVKMAVEGKLPVYFHGRPGGM
                     TPTPAEIVEKAKKIIAGELVAGGAR"
     gene            complement(20901..21116)
                     /locus_tag="KRL67_001420"
     CDS             complement(20901..21116)
                     /locus_tag="KRL67_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HBH1522314.1"
                     /translation="MAKGKVSFNQERCKGCGLCVEACPVKIIQLDSNVINKKGYNPAT
                     VFEMEKCIGCASCATMCPDVVITVERD"
     gene            complement(21159..21836)
                     /locus_tag="KRL67_001421"
     CDS             complement(21159..21836)
                     /locus_tag="KRL67_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBH1522315.1"
                     /translation="MITNDKRIRIITGHYGSGKSEFAMNYVVKLRDMVSGKVAIADLD
                     VVNVYFRTREKKELMKSLGIQPIDSSINAPTLDLPAISAEVMSPMVDHSYNSVIDLGG
                     DNVGARVIGRFSHLLKEGDYDMLFVINANREKTQTSEEVIQYIKEIEKSSKLKVTGLI
                     NNTHLIRFTTIDDVLRGQKVAKEVSEKCNIPIRYVACLENLVEQLPKDLEGEIFPIKL
                     YMREDWM"
     gene            complement(21826..22905)
                     /gene="buk"
                     /locus_tag="KRL67_001422"
     CDS             complement(21826..22905)
                     /gene="buk"
                     /locus_tag="KRL67_001422"
                     /EC_number="2.7.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="butyrate kinase"
                     /protein_id="HBH1522316.1"
                     /translation="MSKIFKILTINPGSTSTKIAVFDNEDLVFEKTLRHSSEEIGKYE
                     KVSDQFEFRKQVIEEALKEGGVKTSELDAVVGRGGLLKPIKGGTYSVSAAMIEDLKVG
                     VLGEHASNLGGIIAKQIGEEVNVPSYIVDPVVVDELEDVARISGMPEISRASVVHALN
                     QKAIARRYAREINKKYEDINLIVAHMGGGVSVGAHKNGKIVDVANALDGEGPFSPERS
                     GGLPVGALVKMCFSGKYTQDEIKKKIKGNGGLVAYLNTNDAREVEERIEAGDEKAKLV
                     YEAMAYQISKEIGASAAVLKGDVKAILLTGGIAYSKMFTEMIADRVKFIADVKVYPGE
                     DEMIALAQGGLRVLTGEEEAQVYDN"
     gene            complement(22927..23829)
                     /gene="ptb"
                     /locus_tag="KRL67_001423"
     CDS             complement(22927..23829)
                     /gene="ptb"
                     /locus_tag="KRL67_001423"
                     /EC_number="2.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate butyryltransferase"
                     /protein_id="HBH1522317.1"
                     /translation="MRSFEEVIKFAKERGPKTISVACCQDKEVLMAVEMARKEKIANA
                     ILVGDIEKTKEIAKSIDMDIENYELIDIKDLAEASLKSVELVSQGKADMVMKGLVDTS
                     IILKAVLNKEVGLRTGNVLSHVAVFDVEGYDRLFFVTDAAMNLAPDTNTKKQIIENAC
                     TVAHSLDIREPKVAAICAKEKVNPKMKDTVEAKELEEMYERGEIKGCMVGGPFAIDNA
                     VSLEAAKHKGINHPVAGRADILLAPDIEGGNILYKALVFFSKSKNAGVIVGAKAPIIL
                     TSRADSEETKLNSIALGVLMAAKA"
     gene            complement(24133..25314)
                     /locus_tag="KRL67_001424"
     CDS             complement(24133..25314)
                     /locus_tag="KRL67_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH1522318.1"
                     /translation="MINRLKNNTKIKIISFLSAIALWMYVMAVVDPEETKGFEDIPVS
                     ISNMSDLKDKNLVIYPDEKLTADVYIKGSLSKVQSVKKEDIHVYGTIEDPIQGQKEVY
                     LKANIPQGVTLEFKHDVLVVNLEKNIKEKRDIKVEVSGNSKRNINKIELSKEFVDVSG
                     PRTLVKEVKSVRANLDVGNETDDFSKKLQLIPLNSDGTKVEGVTLSSSFVTAKIELLK
                     EKIVPIRVSLNDNKDNDVLKNYKLSKEQVTIKGKKEDIDKINYIKTKPIDLADLANGE
                     EKEVSLEIPEGITLEDSSITIKLNDTKQLSAEFLYNAGDIELRNIPTNMDNPNVISDV
                     DIKVVVESTEDLSKLNKKDISLYIDLSQGPEEDSKYKIKYETTYQFKKINIEPNIVEV
                     E"
     gene            complement(25311..26150)
                     /locus_tag="KRL67_001425"
     CDS             complement(25311..26150)
                     /locus_tag="KRL67_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00159 family protein"
                     /protein_id="HBH1522319.1"
                     /translation="MLDINSFLNGFFNIILRMSIYDLIDISIVAYIFYKIFMFIKDTR
                     AEQVFKGIIFLLLATQLSNTFKLHTVYWISLKALDYGVIAALIIFQPEFRAGLEHIGR
                     AKFNLFGKNVNTSEETLNRNIEEIVEALYSLSRQKIGALIIMERETRISDIINTGTII
                     DAEISRQLLINIFIPNTPLHDGAVVIRDSKVKAAACFLPLTESKDLSKDLGTRHRAGI
                     GVSEVSDCITLIVSEETGGVSIAKAGKLYRDISRERMMNILRSNLKTNTETRSFFKGG
                     IFK"
BASE COUNT         8012 a         5117 c         2895 g        10662 t
ORIGIN      
        1 attcttgtat atttttttct acttatacaa tccaataatg gaatatttac tattttactt
       61 aaattaaatg ctattttttg tgcctgatta aaacctcttt tatttagcct ttttttatga
      121 agtggaacaa ataatatata atcaaatttt atgttttcta aatataattt ttctttcatt
      181 atttgagcta tatacttagc cataaaggtt ttttggttat acttcagccc tagcactatc
      241 ttttttgtca catcattgta ttcaatacaa gagattgatt tatcaaaata aaagtcttta
      301 ttaaaacaat aactacactc ttcaataaat tctttttcaa ttgaatgacg tattattggt
      361 tttccacact tcatgcaacc atcttgtata aaattcattt ctttaaaaca acttttacat
      421 attgaataag tatttgtttt ctttatggat ttatcacaaa ttatgcaact tatattctca
      481 ggatatataa aatccaaaca tttattaatc atatttttaa aattcttaat aaaattatca
      541 ttgcatattt tcatatttat tctatcaaca gcccttcttg cttaaattta tttaacttat
      601 atcctaaatt tgaatacctt tgattaactc tattattttt tatcatatat tcaagatatt
      661 tcacatctcc aactaataca actaattttt ttgctcttgt tactgctgta tataataaat
      721 tacggctaag taacatagga ggagcccaag ctattggcaa tactacaact ggaaattcac
      781 ttccctggct tttatgtatt gttgtacaaa aactatggtc aatttcatct aattcatcat
      841 ataggtatga tacaatttta gtctggtcaa ataatatata aatagttttc ttatctttat
      901 ctatgtgata aacatatcct atatctccat tatagatacc ttctccactt tctttttggt
      961 cttctgtttc ccattttttt gtataattat tttttacttg cattacctta tcgccaactc
     1021 taaataatct tttagaaaaa ctttcttcaa ctttaaattt ttcttttttg ttaagatact
     1081 tctgtaactc tatattgaga ttagtaactc ccaattcacc ttttctcatt gaagataaaa
     1141 cttgtatatc ttttaattta tcaactttat aaaatttagg taatctttca ttaaccaatc
     1201 ctattatttc atttaaaatt tcttcattag ttgattttct tataaaaaag aaatctttac
     1261 cttttgtatt tagataaagt ggttctccat tgtttatctt atgggcattt acaattatca
     1321 tactttcttg cgcttgtcta aatatttcat ttaatttaac tacatttata atattagaat
     1381 taatcatatc cttaagtaca tttccagcac ctacagaagg taattggtca ctatctccaa
     1441 ctaaaattac tcttgttcct aactttatag ctctaagtaa attgtacatt aagataatgt
     1501 ctaccattga tacctcatca acaattatta catctgaatt tattgggtct tcttcatcct
     1561 taaaaaaagt taaatcatca tctgttgaaa atcccatttc tagcaaccta tgtatagttt
     1621 ttgcttcttt attagaagtt tcactcattc tctttgctgc tcttcccgtt ggagctgcca
     1681 aagtcacgct tttaccatta ttttcaaata tatctatgat agcattaatg gtagttgttt
     1741 taccagttcc tggaccaccg gttataatta atactccact attaacagat tctttgactg
     1801 ctaaaatttg tttttctgct aacttaattt tcttatcttc ttctaaaaca ttaatctcgt
     1861 tatctatatc tatttttaag tcttcaaatt catactgtga taattttact atttgactac
     1921 atactccatt ttcagctaaa tagtatggca taaggtatat aagattttca ttgtttactt
     1981 tctctatatg tattttttga ttataaacta gcatcattac acactctttt attatttctc
     2041 catttagctc caataatttt tccgaatctt gaattagaac atgctctggt aaatatgtat
     2101 gcccattact taaagattta tttaaagtgt ataagattcc ctgacaaatt ctatccttag
     2161 agtttttgtc tataccaatt ttatttgcta tactatcagc tactttaaat ccaattcctc
     2221 ttatatcttc agcaagctgg tatgggtttt tatttataac ttctattgca ctgcttttat
     2281 attttttata tatttttagg cagtaatttg gtgttattcc aaaaggagat agctgtatta
     2341 ttatatttct caattctctg tcttcttcat aactttttac tatttgtttt acttttttac
     2401 tccctatacc ttctacttct tgtagttttt ctggtgaatt ttgtatcacc tctaatgtat
     2461 ctacaccaaa cttatcaatt attcttttag ccattttttc accaatacca tgtatcattc
     2521 cagaagataa gtaaacataa attccttcta aggatgatgg tgttactggc ataaaactat
     2581 tcacttcaaa ttgagtccca taaatcttat ggtttaccca ctttccttca acttctatgc
     2641 tttctccaat tgccaaagta ggcatacatc ctacaacgac aatttcatca ttttcattta
     2701 ccagatgagc tattgtataa ccattatctt cgtttttaaa gactatttca cttatcattc
     2761 cttctaattt ttccattttt atctcctaaa aatactaata atcgctccat ttaaatgtta
     2821 gtataatttt atatattaaa actatgataa tcgtagaaaa gttttatatt tttatactta
     2881 acgtaatttc caactattat acattttatc agaaacaaaa aaaatctgca tccataaaat
     2941 atggtaccat gaaatacaga taccatattt aaatagctat tttattttaa ttactccata
     3001 tttagagtat aaattaaatt tcataagtat attacagtac tagcttattt cagatttatt
     3061 ctccttgtac ccctcagttt tagtaattct tctataaagc atacatagta aaattaaaat
     3121 tcccatgtaa cctgtatatg gatatagtgt acctactaac ttatcaaatg gcagtagacc
     3181 tcctatacaa gctaatatac ttactactat tgttataatt ttaaatttag ggtggtcatc
     3241 ttctacaaat cggttagtta ctgaccataa caatggtaca gctgtagtgt atattcctag
     3301 taaaagtact actgagaata aaatacctat tattggagat atcttgtcag ctaagtaaag
     3361 tgctggtatc tcttttgtat atatatttcc tatgtctgaa agtaatgcca aattcatcat
     3421 tatagctgct gccataagtg ctactccacc tactatacca ccccacacag catctttctt
     3481 atttactgca gaagctccca ttcctgtcaa gaatgttatt actattatta cattataaca
     3541 agtataaagt actcctgaga aatatccatt tgatgtagca gttttaatat ttaaactatc
     3601 aaccatattt ccagcatttg ataaagcatc aatattgcta aataaactaa ttgctcctac
     3661 taaaagtgta aatattatta ttataggtcc tatattacct agtattttag aaagccttgt
     3721 caaacctaat gaaacagtta ttagggaaac gattgccata ccaatcctac ctacatatgg
     3781 atttaatcca tagtactctg ataatgtagc tcctgctcct gcaatcatta ttactaatgt
     3841 tccaaataaa aacaatggac caaaccattc aaaaaatgtt ccaagatatt ttccacaata
     3901 tacttgataa atttttattg gctccttaag tttcaattct cttcccttat ctataacttc
     3961 tgctccaaac catgaaaata gtaccataga gattattcca cctataatac cagcatatcc
     4021 ataaaacgta aaaaattgca gtatttcttg accagttgca aatccagaac ctattacagt
     4081 agcaacataa gctccagcaa aacttataac cgttttaata tttactttgc ctgacataca
     4141 ctactccccc ccaattaaaa ttatttttaa agtgtaagtt tattaagatt atattagact
     4201 cattattgtg atatttcaat atatttttaa ttatttaaca ttagttaaca catttttaac
     4261 catcctttaa catatatccc ataatatgca taaatttagt atagtattat ataaaatgta
     4321 ttgaccagat taaatactgt ttattcatta tatgatgata aattaatatt tattaaaaat
     4381 atttaactca taataaaaaa gctgttagaa agtaatcata aaattacatt ctaacagctt
     4441 tttagttaaa aattattcac ctaaagcatc ttttcttaac tgctcaacct tgtcagttct
     4501 ttcccaaggt aaatctatat cagttctacc aaagtgacca taagcagcaa cctttttata
     4561 aagaggcttt cttaaatcta agtctcttat tatggctcct ggtcttaaat caaagtgctt
     4621 atttactaat tcaacaagtt tttcttctga aactttgcct gtcccaaaag tatctataaa
     4681 tatagataat ggtctagcta tacctatagc atatgcaagt tctatctcac atttgtctgc
     4741 aagaccagcc gcaacaatat tttttgcaac atatctagct gcataagcag cagatctatc
     4801 aacctttgta gggtcttttc ctgagaaagc tcctccacca tgtctagaat atccaccata
     4861 agtatctatt attatttttc ttcctgtaag acctgtatct ccttgtggtc ctcctataac
     4921 gaatcttcct gttggattta tataaatttt agtatcttca tcaagtaatt ctgctggtat
     4981 aacttcttta ataacatgat taattaaatc ttctcttatt ttatcatttg atactgtttc
     5041 acaatgttga gctgatataa gaactgtatg tactcttaca gctttacttc cttcatattc
     5101 aacagtaact tgagtttttc catctggtct taaataatca actagaccag tctttctaac
     5161 ctctgtaagt cttcttgata atttgtgtgc taaagatatt ggaagaggca ttaattctgg
     5221 agtctcatta catgcaaatc caaacattat accttggtct ccagctccaa ctttttctat
     5281 ttcttcttct atctcttctc cagtcttact ttctaggcct tcatcaactc ccatagctat
     5341 atctccagat tgttcatcaa tagaagttat aactgcacaa gtatcacaat caaatccata
     5401 ttttgctctt gtatatccaa tttctcttac tgtttctctt actaatttag gaatgtctac
     5461 ataagcagat gtacttattt ctcctgctac taaaactaaa ccagtagtaa ctgtagtttc
     5521 acaagctact ctagattgtg ggtctttttc taataatgca tctaatattg aatctgatat
     5581 ttggtcacat atcttatcag gatgtccttc tgtaactgat tctgacgtaa ataaatgtct
     5641 tgccattttt attccccctt atttattctt agtttttatt ataggttttg ccttgttttt
     5701 aattttagaa atttactcaa aataaaaaac ctctccttta taagaagagg ttaataatcg
     5761 taattacata ctaattaact gacctcatct ttcaagactt ttgtcttgta ggaattagca
     5821 ccttttccca tttgggtggt tgctgggctt cacagggcct atccctcagc ctctcttgat
     5881 aaggtaatca agatttaagt tttgttttac tttactagct tactattttt ttaaaatttt
     5941 gtcaatattt ttagtcatat tttttctatt cctttttttg taagacaaat aaaattttct
     6001 atctatctga aatttaaagt taggtctttt taatgctttt tattcatata tttttttgaa
     6061 gggggtgttc ctattgtatt tagctaaagt aaattacagc gatgatgatg ttatacagat
     6121 gctaagttca gttctaaaaa ctataattaa tgaaaatacg attgttgttt gcataggaac
     6181 agacagggct ataggtgata ctttaggccc tttagttgga acaatactta agaacagtaa
     6241 ctttaaatac cccgtttacg gtacactaga taaccctatt cacgccttaa atatatatga
     6301 gtctttagat actataaaaa atacacatat acaaggtaac ttcttggcta tagatgcttg
     6361 cttggggtcg cagagtaaca taggtaatat acagattaga gaaggaccta tcctgcccgg
     6421 aaaaggtgtt ggcaaaaaac tacctcaaat aggaaattat tctatagtag gaattgtaga
     6481 taaaattgat gaaaataata aactttcatt taataatata cgtcttagct ttatattaga
     6541 cttagctgaa acaatagcct tggctttatt agtatccacc taatctaaat ataaactaaa
     6601 tgataaaatt ctttatataa aaaggtagat attttagagt atctgattaa atactccaaa
     6661 ttatctacct ttttactttt atatttcaag tttttatttt tctactaagg caaacattaa
     6721 ttccccttta cagacaagtt cttcaccaac atatgcctta gcatctgcct tggctatatt
     6781 tcttctaaga gttgtcattt caacttccat agttaatgta tctcctggtc ttacttctct
     6841 cttgattcta actttatcta tacctgcaaa tacacctagt tttcctttgt tttcctctac
     6901 agacatcatt gccactccac acacttgtgc taaagcttct actattaata ctcctggcat
     6961 taaaggatat tctggaaagt gaccttggaa aaatggttca ttaactgtta cattctttat
     7021 accaacagct cttttcccaa cttctagctc tgtaatcttg tccactaata aaaaaggata
     7081 tctatgtggt attaattttt ttatttcatc tatatttaac attctaaact ctcctaatta
     7141 ttttcttttg aaagacccgc cagtttcaag taggtctatt gaacctaaag ctattcctgt
     7201 accttttgca acacaagata atggttcatc tgctattgta acagttattc ctgttctttg
     7261 ttctattatt ttatctaatc catataataa tgaacctcct ccagtcatta ttataccaac
     7321 atctcctata tctgctgcta actcaggagg cgttttttcc aaaacagcat gagtagcaac
     7381 tactatttgc tcaacacatt ctctaagtgc ttctaacatt tctgttgaat ttatagttat
     7441 agaactagga agtcctgtaa ttaaatttct tcctgttatc ttcatatatt tttcttcttc
     7501 tctcttaaat gcagaaccta tttcaaactt gattttctct gctgttcttt ctcctataag
     7561 aagattgtgt tgttttttca tatatttaac tatagcatca tcaaatctat ctccaccaac
     7621 ctttatagat tcacttacaa cagctcctcc tagagatatt acagctatat ctacagtacc
     7681 tccaccaata tctataatca tattaccgtt aggctgtgat atgtctacac cagctcctat
     7741 ggctgctgct attggttctt ctattatata tacttctcta gctccagctt gtctcgtagc
     7801 atcttctact gctctttttt ccacttctgt gacacctgtt ggtacgcata ccataattct
     7861 tggcataaaa aatcttccaa atcccttttt atcaactatc ttatctataa aagattttaa
     7921 catcttctca gttacatcat aatctgaaat tacaccatct cttaaaggtc ttatagcgac
     7981 tatattacct ggtgttctcc ctatcatctt tttagcttct tctcccacag ctaaaacaga
     8041 atctgttcta ttgtctatcg ccacaacaga tggttcttct aaaacgattc ccttaccact
     8101 aacataaaca agcacatttg cagtacctaa atctatgcct atatcagctc cagccatttt
     8161 ttcaactcct ttatttataa tattagatta tattataagc ctattttaat actcctataa
     8221 taaagcatat acaaaaatct cgctttaatc aagagaaaat aaatcttttg agagtattat
     8281 tattttgcta tttataatta cttaatatat ttctttttat aatttaatta aataaaaaaa
     8341 gtaggttttt aacctacatt tttttatgtt ccctctcata ttttaatttc gttgctaatc
     8401 cccctctaat atgtctctct gatttatttt tgtccagcac atttttaact tctttggcaa
     8461 gggatggatt tatttccttc aatctttctg taacatcctt atgaattgta cttttactta
     8521 ctccaaatgt cttggcggtt tgtcttactg tagtgttttt ttcaagtata tattttgcta
     8581 ccactatggc cctttcctct atatgagatc tcaaagatta cccccctcac ataatagctt
     8641 taatgatata tatgtaaaaa caccaaaaaa tataacaagc ttaaccaaat atgattaaac
     8701 ttgttatact tttacttaat tagactcatt gggtctatag atttttctcc actatacgcc
     8761 tctaaatgaa catgtatacc ttcttcactt tctatttgag aagtactacc tacagtacct
     8821 agagattgtc cttctgttac cttctgttct ttcttaacac taacattttt atctagattt
     8881 gaatatacta ctacaatgtt attgtcactt tttattttga ctgattgccc atattcttca
     8941 tcattaaata catctactac tattccgttt aatagagatt ttacttcttt gcctttagta
     9001 caactaatat ctacaccttt atgagtttcc caaacatcaa gtgttttaga ataactaggt
     9061 tctttttctg aatatcctct tattacttta tctccaatgt aatttaattt tgttgtactc
     9121 gatttatttt cctcttttga cttagctttt tctaagtttt gttttgaatc cgttgcagta
     9181 gggatagcat ctttcttgtc tttttcaatt aaatgtattt cctcttcact atctttatta
     9241 gcattttcca taattccttt attagaagcc aatttatcta cattattatt tgtaaaccaa
     9301 actccaccta ctgctaataa acaaacacat acaaacaaag ataaataaaa accatctttt
     9361 tctaacagct ttttcttcat tttgcacctc caaatatatt tctacatttg gattattgac
     9421 gtgtttttgg cattttatac aattattagt atatatcttt tatttttgta tctgtatagt
     9481 aatgtgataa tatatcataa tatttatagc cctcttctgc cattccttct gctccccact
     9541 ggctcatacc aacaccatga ccatatcctt ttaccacgaa atctatgtat ccttctccaa
     9601 attttatgtc aaagtttgca gagttcaatt taaaaacagt tctaatgtct tttccagtca
     9661 attctttatt tcctaatttt attttttcta cagttccggc atcacttctt ttagttattg
     9721 aaacttgttt acttaaatta ttcacatcta tcacaatagt gctatatgcc cttctaagac
     9781 ttttaacaaa atctgtgttg cttattttaa gtgttgacgc aaatttaggg gaatacttat
     9841 cataaggact ttccacagac tttaaataag gatattttgc agaaaatact tcttcactat
     9901 tttctgtttt tcctgaagat gtagaaaaat aaagaggaag tatcgcctta tcattataag
     9961 tcattatttg acctttagtt cctctaactg cttcttggat tttggaatat ttattcttaa
    10021 tccattcttc accatttaat tttttaagag tatcataact cttatattct tgacaatgtt
    10081 tataatcagt acatactact gcatttttat gtttactaga tttaccatgt tcctgtttat
    10141 atactacata tgttctagct gctacagatt gagcttttaa agcttctata tcaaactctg
    10201 aagacatctc accagctagt actccacata aatagttctc catgtccatc ttttcggttt
    10261 tccctgttat atgattatat acattgataa ttggtacttt tttatttaca gtttcatagt
    10321 tcactgtatt tttttcttct ttattacctt tgtcttcaat atcacttttt ttaatagttt
    10381 tgtttataga tactgggctc tctgcttttt cagttaattc tacattttta taagaaacta
    10441 aagttataag tgatggaact aacacagagc aagtcacaaa tcctaacaaa acaaccaatg
    10501 ggttcttcat atactctcct ccttagcttt taaaaccttg tatttaagta tatgaacaac
    10561 cctattaatt tataactttc ttctataaac caattattaa tcttccacta tttcgatttg
    10621 accacctaat gctgttatct tcttatctat atcaacatag cctctttgga tatggtatat
    10681 ttctcctatt tgagtttctc cttctgctat aagaccgcat aaaataaggg ctgcccctgc
    10741 tcttaaatca gtagcgttta ctgctgcacc gtgtagctca tctacaccat taacaaccgc
    10801 acttcttcca tctattttta tattagcacc cattctatta aattcagcaa catgcataaa
    10861 tctgttttca aaaacagttt ctataactac accagttcca ttagctacag taagcatagc
    10921 cataaattgt gcttgaacat ctgtaggaaa tcctggatgt ggtaaagttt ttatatctat
    10981 tggttttaat acttttggac ctacaactct tacagaattg tccatttctg ttatttcaca
    11041 accagcttct cttaacttag ctacaactgg ttttaaatgc tccattaaaa cattttcaac
    11101 agttatatca ccttttgtca tagctgctgc aaccatataa gttgctgctt ctattctgtc
    11161 tggaattaca ttatgttctg ctccttttaa ctctttaaca ccttttattt ttatagtatt
    11221 agtacctgca cctttaacat ctgctcccat ttcgttcaag aagtttgcta aatctactat
    11281 ttctggctct tctgctgcat tctctattat tgtagtccct tctgctaaag aggctgccat
    11341 cattatgttt tcagttgctc caacagatgg gaaatctaaa tataatttat ttcctactaa
    11401 cttttctgtt gctgcttcaa caaaaccatg gtccatttct atctttgcac ctaaggcttt
    11461 aaatcctttt aaatgtaaat ctataggtct tgttcctata gcacatcctc ctggcataga
    11521 aattttcgta ctattaaatc tagcaagcaa tggccccata accaaaaaag aagctctcat
    11581 ctttcttaca agctcatatg gagcttcaca agtttttata ttacttgcat caacagtaag
    11641 tgtattttct ttatattcaa cttctgcccc tacatgtctt aataaatctg atataacatg
    11701 aacatctctt aaatttggta ctccatttaa tgttgatttc ccgtttgcta ataaagtcgc
    11761 tgctattatt ggtaaaactg cattttttgc accatctatt ttaacactac ctttaagtgg
    11821 attactcttt tttactatta tcttagccat attttttgat ctcctcataa ttaataatct
    11881 aattttataa ttggtgtggc aatatatatg agtgttcttc cgtcgtcctc attatattta
    11941 attcctatat ttaaattaat tttttcccct aaatattcta attcataatc atttaatact
    12001 ttactatatg ctgttataga taaaaagttt acgtctttca cttcatctat ttgcttagca
    12061 ctcatattat ataatatttt ttgtaaaata tcatcatatt tgttgttttg taaccttttt
    12121 gtgtattctc caacaataca agcagacaca tctacttgag atgaatattt atttaacaca
    12181 ttttctacgt tactataaat aacctcttta tgtttatata ctttgttatc caatatgtca
    12241 actactatgt aactttcttt gttattttta tttattcctt ttatagaaat ttctttatcg
    12301 ctacctttta tagtagcaca taattgtatt ttatcgccag accctttttg attccaattt
    12361 attccttttt tatccaactt tagattgtta attatttcta taaaaatatt atgaaattct
    12421 ttatttgata aattatgatt tataacagaa ttaattttaa cattataaaa tttaaaattt
    12481 gcttctgaga tttcaagagc tttacttaat ctattgtatc tagtatttat acttgtatct
    12541 gcataagata ttgtcatccc tataatcatt aatataaaaa agcttatagc taattttact
    12601 cttttcatag taaaaaatcc cccttaaata tgtttttctg tataatagaa ttattaacaa
    12661 atttaagaga gatatacttt ctcaatatgt atttttatac atctttgtat ttttcatcta
    12721 ataagtctct ttcaaactct tcaatctcaa ttttctctat ccttattcta gatttgccaa
    12781 ttttttatat gtatcaaata catcttgcac tagttcttat atattctctt ctgtaatata
    12841 aattacttag tctatactta aatctataat ctataatcta taatctataa tctataatct
    12901 ataatctata atctattcac attttaccag caaaataaac agataatact tttttatcaa
    12961 tttccatagc tccaaacgaa catacctaag tacacttcca aaaacacaat ttacacactc
    13021 ttcctaataa atcaaaaaca cctttgtttt aaacaactct atcgtacttt caaaaacttt
    13081 atttgtgttc tcccacttca attaaatttg aattatcaag actccagccc atccatccat
    13141 cactataaag ggaggtattt tccactccta tgacatttgc atatgttaat acttcagcag
    13201 cacgccatcc acttccacac ataaatataa gctgtttatt cacatctata ttttccttgt
    13261 cccacatttc taaaatctca tatttatttc tcattgtatt atcaatatta cgatattctt
    13321 ctagtgaaac actatcacta cctgaatgtc catataaagc tcctggtata cgcccttttt
    13381 tatcataata tgtatatcca gatacttttc ctatatgttc atcccaagta cggttatcta
    13441 ctagtataaa cttatttttt tcttttagtt tttgtcttaa ttcagaggta gtaacaacta
    13501 attgtgagtt aactggtata tctgctccaa aattagtaca tgaatgtttt ggattactag
    13561 taaattctaa ttcatatcct gctgataacc atgagtttgt tccaccattc aaaactctta
    13621 catcttttac tcctatatat ctcaatataa cagctagtct ataagatgcc ataggtgtac
    13681 tacttgaaac tattacagta tcatcctttg ttaatccata gtctaaagca aacttaatta
    13741 agttatcatc attatcaagc atccatgttg gaggaggttc tataatatcg gtatttacat
    13801 gaacacttgt aggtatatgc ccttttgtat acgattcttc tccccaactc gcttcaatca
    13861 tttttatatt ctttgaatct tcaaatgtct caggaatttt tccatctaat atatctttta
    13921 ttataaaagc aggaactatc atttgataat ttttatatct ttccattgga agattttcgt
    13981 tgtctgccca ttgtttaata tcatatttat atagatattt atatcctttt ttagataaat
    14041 aatctgctac aaccaaagca tcttttccat ttgcatcata taatactata tttttatcta
    14101 aatcaattct tttagttttc aatgcttgtt ctagcacttc atcttttcta tctgagtata
    14161 cacttaacca attagcagaa aaatctactg ctccttttat atgtccacct ctttttactc
    14221 catctaactt ccagccatta taagcatcat ttaagcgagt atcaactata acccaagcgt
    14281 tatcttgcac tttttcttgt aaaacttcag tagatattgc cttaagatta acaaaataat
    14341 cttctttctt ttctttttta aaatcatttg tcataattta tactcccaat ctaaaaattt
    14401 ttttatttaa aatcattttg ctaaaataaa gtaattttct tatactctat tagtttcatt
    14461 atattctttt ataaatagtt ccttatattc ttattttaaa ctactcactt tattttatca
    14521 caaggttact aataccttat gtattttaat ctttctcata atctattatt ttttatgtct
    14581 gatattgact atatgtttat taagtattca ctatatctga tatcgactat atgtttatta
    14641 agtactcact atactttata taaactatat atttattatt ccctatacct tatatggtct
    14701 atatatttat cattttacag atgatttaat atcaatcttt ttataaaatt tattatagca
    14761 aaaacaaaac aaatttaaca tttatctaaa aaactcatcc tataaaatac aaataacagg
    14821 atgagttctt tttaataaat attatctatt taatttttaa tctgttttta ttcaactgtt
    14881 actgatttag ctagatttct tggcttatct acatcgcatc ctcttaatac agagacatga
    14941 taagcaagta attgcattgg tacaactgct aatattggtg ataaaatatc atctgaattt
    15001 ggtatataga ttattttatc agcagctttc tcaacatcct tattatggct ttgagctatt
    15061 gctactacat aagctcctct tgctcttact tcttccatat tagaaaccat tttttcaaat
    15121 aacttttctt gagtagctat ggcaattaca ggagttcctt tttctattaa agctattgtt
    15181 ccatgcttta actctcctgc tgcaaaagct tctgcatgta tatatgaaat ctcttttaat
    15241 ttcaatgaac cttccatcgc taagctatag tctatacctc ttcctaaata gaatgcatgt
    15301 tcagaactaa caactgtttt agcaacttct tttatatatt cttcattgtc tagtatttct
    15361 tgtatttttg atggtatttc tttcatctta gaaatcatac tatcataaaa ttctctagtt
    15421 attgttcctt ttttgatagc aaagtctaaa gctatcatgt ataaagaagt tatttgagta
    15481 gtatatgcct tagttgatgc aactgctact tcaggtcctg cccaagtata gaatacatca
    15541 tctgattctc ttgctataga tgaacctaca acgttagtta ttgaaagtat tcttgctcct
    15601 ttagcctttg aatctcttaa tactgctaga gtatctgctg tctcaccaga ttggctaact
    15661 aatattacta atgaattttc atctacaaac gggtcgctat atctaaattc tgatgctata
    15721 tcagttatta ctggtatatt tacaaatttt tctatggcat acttaccaag taatccagca
    15781 ttatatgctg ttccacaagc cactatataa actttattta tcttttctaa gtcttcttta
    15841 gaaatattta tactgtctaa gactatattt ccattatcat caagtcttct ttctaaagtt
    15901 tctctaactc ctgttggttg ctcatgaatt tcttttgaca taaagtagtc atatccacct
    15961 ttagacgcag cttctacatc ccaagttaca tgaaatactt ctttttcaac aagatttcta
    16021 ttagagtcat aaactgttac attttcgtct tttagatgaa ctacttctcc attttctaag
    16081 aaataaacat ctctagtata ttttaataat gcaggtatat ctgatgctat aaagttttca
    16141 ccttcaccaa cacctactac aagaggacta tcttttctta ctgcaactaa ttcatttcca
    16201 tgttctttac atataacacc taatgcataa gctcctctta attttgatat agttttgtat
    16261 acagcatcta ataaattacc ctcatagtat ttatctacta aatgtgctat tacctctgta
    16321 tctgtttgag attcaaactt aacaccttca gaaattaatt cttcttttat ttccatatag
    16381 ttttctatta tcccattatg aacaactgct attgttttgg cttgattaaa atgaggatgt
    16441 gagtttacat cagatggttc tccatgagtt gcccatcttg tatgccctat ccctaaattt
    16501 ccatcaatag gattcttttc taagtcttct gcaagtacag aaagtctacc tttaaacttt
    16561 ctaatattaa gttcttcttc attagaacta ttaactgcta ctcctgctga gtcataacct
    16621 ctgtactcta gcttagaaag cccttctact ataacttctg ctgcctttct acttcctaaa
    16681 tatccaacta ttccacacat tatttgtgtc ctcctaaaaa tattatttat atattttaag
    16741 agttaggtca tataagttta ctttggcttc cctttgtcca taggattgct cctattattt
    16801 aaagaagtct ttcgatggta aacacaccgc agagcatccg ccgacaattc gattaactct
    16861 gacctcgtca acttagttcc tatattactc aataattatg tctataacta agttctggcg
    16921 cttagaaagg tgtcctcagt atttatatct gagaataccc tttaaattac gataattttt
    16981 cttgtataag attagctaaa tttgttgcta attcttttat ttgaccttcc tctttaccct
    17041 ctaacataac tctaactaat ggctcagttc ctgatggtct tatcaaaact ctaccatttc
    17101 tgtctaaaat agattctatt ctttctatct cagtttttat ttctggatat tccatatatt
    17161 tgttcttatt ttcatttttt attgttgcat ttactaatac ttgtgggtat tgactcatta
    17221 ttgatgcaat ttctgataaa ggtttctttt cttgtaagat tatatttgca agtattagag
    17281 aactaagcac gccatctcca gtagtgttgt aatctaaaaa tatcatatgt ccagattgct
    17341 ctccaccaag attatatcca tttttaacca tatcttctaa aacatatctg tctccaactg
    17401 ctgtagtaga taaatttatt ccattttcct tagcagctat tgtcaaccct atattactca
    17461 taactgtaac caccaaagta tcttgtgcca atttattatt tttctttaaa tttaaggcac
    17521 tcaatatcat tatatggtct ccatcaacaa tttgaccctt ttcatttact gcaattaatc
    17581 tatcagcatc tccatcatat gcaagaccta agtcagcatt atgttccaaa acagcccttt
    17641 gaagttgttc tggatgtgta gagccacatt tatagtttat attatttcca tctggtgaac
    17701 tatttattga aattacactt gctcctagtt catcaaacac tattggtgct actttatatg
    17761 acgcgccatt agcacagtcc aaaactactt ttaatccctt aaaatcagtg cttattattg
    17821 attttaggta gtctacatag tctctttgtg cacaatgttc atgtaatttt cttcctacat
    17881 tttcaccaat tggaagacaa tcaattttgt ctatatcgtc tatatattct tctattttca
    17941 attcaatttc atcatctaat ttatatccat tcttattaaa gaatttgatt ccgttatatt
    18001 caacaggatt gtgagatgca gatataacca caccacaatc agcaccatat tttctagtta
    18061 aatatgccac tgcaggtgtt ggtattattc caacagtaat aacatcacat ccaacagaca
    18121 taagtcctgc tattaaagat gcttccaaca tatcacctga tattctagta tccttaccta
    18181 ctactacttt tactctgtgg tctccttgag ctaagacaaa cccaccagct ctaccaagtt
    18241 tatatgctaa atcacatgtt agttctgtat ttgctactcc tctaactcca tctgttccaa
    18301 aatactttct cactaaagat tctcctttca taattgaaaa gcttgtaatt tgtattatat
    18361 ttttatagtt cataaaatat cattataggc ttaatttcag tcataaaaat attttaatac
    18421 ttatcagcaa aaaagacaat agaaataatt ctattgtctt ttttttgaca tctttttctt
    18481 tattagttag ttgctttaga acatgtagcg tccatacctt tttgtaaagc ttctctgttt
    18541 aatggaacga atttttcttt tctaggtccg aaaacttttt tgaatgcttc tagtacagat
    18601 tctatatcta cagtatcaga tacttttaag taagcaccta acattatcat attagcaact
    18661 ttatcatttc ctaattcagc tgctaattca tttgctggta tataataaac atctacatct
    18721 gttctatcaa ctttttcttt tattaaagaa ctatttacta ttatttttcc accaggaata
    18781 acatcatctt tgaatttttc aaatgctggt atatttaata atatagctgc agttgcatca
    18841 tctgttataa gtggagaacc aactggttca ttagatacag ttactgcaca gtttgctgta
    18901 cctccacgca tttctggtcc ataagatggt aaccaagaaa cttcttttcc ttctagcatt
    18961 cctgcataag taagtaattg tcccatagac ataacacctt ggcctccgaa gcctgcacat
    19021 attactctag ctgtagacat ttacttcacc tcctcaactt caacattttt gaaatttcct
    19081 aatggatagt atggtatcat gttgtctctt aaccattgta aagcatcgtt tggagctagt
    19141 ccccagtttg taggacaagt agacaatact tctactattc caaatcctaa tcctgcttgt
    19201 tgaacttgga atgctttctt tatagctttt ttagcttgtc taacatgagc tggagtatca
    19261 actgctactc tttctacaaa tacagctcct gttaatgtag ctaacatttc acttactctt
    19321 attggataac cttgagtttc agcatttctt cctgattgag cagtagtagc tctttgtcca
    19381 actaatgtag ttggagccat ttgtccacca gtcataccat aagttgtatt gtttacaaat
    19441 atagtagtta ttttttctcc tctagcagct gcatgaacta tttcagcagt accaattgaa
    19501 gctaagtctc catcaccttg ataagtgaat actgtatttt caggatgaac tctttttatt
    19561 cctgtagcag cagctggtgc tctaccatga gcagcttctt gagtgtcaca gttaaaatat
    19621 ttatatccta aaactgaaca tccaactgga acaacaccta cagcatcacc taatactcct
    19681 agctcttcta atacttcacc aactaatcta tggattatac catgtgtaca tcctggacag
    19741 tagtgagttt gagtatcttg taatccttca gttttcttaa atacaacagc cattatctag
    19801 cacctcctgc aaccaattct ccggcaataa tttttttagc tttttcaact atttctgctg
    19861 gtgttggagt cattcctcct ggtcttccat ggaagtaaac tggtaattta ccttctactg
    19921 ccattttaac atcttccacc atttgtccca tactcatttc aacagtcaat atgtttctag
    19981 cattaggaat ttggttgaat gcttcgaatg ggaatggcca taatacttta ggtcttataa
    20041 gacctgcttt tattccttct tctcttaata tatcaattgc gctttttact actcttgaag
    20101 tagttccata agctgcaaat acaaattcag catcttctgt tttgtacatt tcgtattgta
    20161 cttcattttt ttccattgca ttaaattttt cttgtaaatg ccaacaatgg tcttctagta
    20221 cttctggctc taagtataga gagtttatta cattaggttc tctttttcct ttagttccaa
    20281 cagttgccca atctttagga ggcaattctc ttttcttctc aggagctcta aattcaactg
    20341 gctccatcat ttgacctatc ataccatctg caactaccat aactggtgat ctgtaataat
    20401 ctgcaacgtc aaaagcttcc attatcatat caacagcttc ttgaactgta gctggtgcga
    20461 atactggagt tctgtagtct ccatttcccc cacctcttgt agacataaag tagtcagctt
    20521 gtgaaggttg tatacttcca agtcctggac cacctctcat tacattaagt acaacacaag
    20581 gaacttctgc tcctactgca tatgttatac cttcttgctt taaagcaact cctggtgaag
    20641 atgaagaagt cataactctt gctcctgcac ctgctccacc ataaaccatg tttatcgcag
    20701 aaacttctga ttcagcttga acgaacgctc cacctatttt aggtaactct cttgataaat
    20761 attctggtaa ttcactttgt ggagttattg ggtaaccgaa gaaatatttg caaccagctt
    20821 ctatagctgc ttttccaaat gcttcgttac ctttcataag tatcttagcc attaaaattc
    20881 ccccttaaat attaattaaa ttaatctctt tctactgtta taactacatc tggacacata
    20941 gttgcacaac ttgcacatcc tatgcatttt tccatttcaa aaacagttgc tgggttatac
    21001 ccttttttgt ttattacatt tgagtctaat tgtatgattt ttactggaca tgcttcaaca
    21061 cacaatccac aacctttaca acgttcttga ttaaaagata cttttccttt agccatttac
    21121 ctaagcctcc ttaaactttc tatatatttg agctcaaact acatccagtc ttctctcata
    21181 tataatttta taggaaaaat ttctccttct aaatcttttg gtaattgttc aacaagattt
    21241 tctaaacatg caacatatct tataggtata ttacattttt cagaaacttc ttttgcaact
    21301 ttctgacctc ttaaaacatc atcaatggta gtaaacctaa taagatgagt attattaatt
    21361 agacctgtta cctttaattt agaagatttc tctatttctt ttatatattg gataacttct
    21421 tcagaagttt gagttttctc cctatttgca tttattacaa aaagcatatc ataatcgcct
    21481 tcttttaaca aatgactaaa tcttcctata accctagcac ctacattatc tccacctaaa
    21541 tctataacag aattgtatga atggtctacc ataggagaca ttacctctgc tgatattgct
    21601 ggtaaatcta atgttggagc attaattgag ctatctattg gttgtatacc taaagatttc
    21661 attaactctt tcttttctct agttctaaag tatacattta ccacatctaa atcggctata
    21721 gcaactttgc cagaaaccat atctcttaat ttaacaacat agttcatcgc aaattcactt
    21781 ttaccacttc catagtgacc agttatgatt cttattcttt tatcattagt tatcataaac
    21841 ttgagcctct tcttcaccag ttaaaactct aagtccacct tgagctaatg caatcatttc
    21901 atcttcacct ggataaactt ttacatctgc tataaattta actctatctg caatcatttc
    21961 tgtaaacatt tttgaatatg cgattccacc agttaataat attgctttta catctccctt
    22021 aagaactgca gcactagctc ctatttcttt agagatttga tatgccatag cttcatatac
    22081 taatttagct ttttcatcac cagcttcaat tctttcttca acttctctag catcattagt
    22141 gtttaagtat gcaactaatc caccattacc ttttattttc tttttaattt catcttgagt
    22201 atattttcca ctaaagcaca tttttaccaa tgcacctact ggtaatccac cacttctttc
    22261 tggagagaaa ggtccttctc catctaatgc gtttgcaaca tctactattt taccattttt
    22321 atgagctcca acagaaactc ctccacccat gtgtgcaact ataagattta tatcttcata
    22381 tttcttgttt atttctctag catatcttct tgctattgcc ttttgattta aagcatgtac
    22441 tacacttgct ctacttattt caggcatacc agaaattcta gcaacatctt ctaattcatc
    22501 cacaacaaca gggtctacta tgtatgaagg aacatttact tcttcaccta tttgttttgc
    22561 tattattcca cctaggtttg aagcgtgttc tcctaaaact cccactttta aatcttcaat
    22621 catagcagca cttactgaat aagtaccacc ttttataggt ttaagaagtc ctcctctacc
    22681 tactacagca tctaattcag atgtttttac tccaccttct tttagagctt cttctattac
    22741 ttgtttacga aattcaaatt ggtcagacac cttctcatat tttcctattt cttctgaaga
    22801 atgtcttaaa gttttttcaa atactaaatc ctcattatca aatacagcta ttttagttga
    22861 tgtcgaacca ggatttattg ttaagatttt aaatattttg ctcatttgcc tatttccccc
    22921 ttaaaattaa gcctttgctg ccattaaaac acctaaagct attgagttta gtttagtttc
    22981 ttcactgtct gctctagaag ttaatattat tggtgcttta gccccaacta taactcctgc
    23041 attttttgat tttgagaaga ataccaaagc tttatataat atgttaccac cttcaatatc
    23101 tggggctaat aatatatcag ctcttcctgc tacaggatga tttatacctt tatgtttagc
    23161 tgcttctaaa gatactgcat tatcaattgc aaaaggtcca ccaaccatac aacctttgat
    23221 ttctcctctt tcatacattt cttctagttc tttagcttca actgtatctt tcatttttgg
    23281 atttactttt tcttttgcgc atattgcagc aacttttggt tctcttatat ctaatgaatg
    23341 tgctactgtg caagcatttt ctatgatttg ctttttagta tttgtatcag gagctaagtt
    23401 catagctgcg tcagttacga aaaataatct atcgtatccc tctacatcaa atactgctac
    23461 gtgacttaat acatttccag ttctaagacc tacttcttta tttaaaactg cttttagtat
    23521 tattgatgtg tctactaagc ctttcattac catgtcggct tttccttgtg aaactaattc
    23581 aacagatttt agagatgctt ctgctaaatc ttttatatct atcagttcat aattttcgat
    23641 atccatgtct atgctttttg caatttcttt agtcttttct atatctccta ctaaaatggc
    23701 atttgctatt ttttcttttc tagccatttc aactgccatt aaaacttctt tatcttggca
    23761 acatgctact gatatagttt taggtcctct ttcttttgca aacttaatta cttcttcaaa
    23821 acttctcatg catttccacc tcatcttgaa ttttttgaaa tagaactttt tatatctcaa
    23881 ttaaattata actcgttcag caacgttttc ctagagataa ttgaaatatt taattcaaaa
    23941 tatttattta aatctattta tcagtctttt ttcatatatc tagcgctttt tagatttaac
    24001 aaatatctca ttgtccttat tttataatat aactaaaata aacaagatgc aacaaaaaca
    24061 taaaattaac aaacttaatt ttataggttt ttttgtcaca tcttgtaaca actaaatatt
    24121 cgaaatattt gttcattcca cttcaacaat attaggttct atatttattt ttttaaattg
    24181 atatgttgtt tcatatttta ttttatattt actatcttcc tcaggacctt gtgataaatc
    24241 aatatataat gaaatatctt ttttatttaa cttactcaaa tcctccgttg actctacaac
    24301 aacttttata tctacatcgg aaattacatt tggattatcc atatttgttg ggatatttct
    24361 taattcaata tctcctgcat tatataaaaa ttctgcactc aattgttttg tatcatttaa
    24421 tttaattgtt atagagctat cctcaagagt tataccctct ggaatttcta agctaacttc
    24481 tttttcttca ccattagcca aatcagctaa gtcaataggt tttgtcttaa tataatttat
    24541 tttgtctata tcttctttct ttccttttat tgtaacttgt tctttactta atttatagtt
    24601 tttcagtaca tcattatctt tattatcatt tagacttact cttattggga caattttttc
    24661 cttcaatagt tctatctttg cagtcacaaa agatgaactt aatgttactc cttcaacctt
    24721 agttccatca ctatttaatg gtattaattg caatttttta gaaaaatcat ctgtctcatt
    24781 accaacatct agattagctc ttacactttt gacttcttta actagagttc ttggacctga
    24841 tacatcgaca aattccttac taagttcaat tttatttata tttctttttg aatttccact
    24901 tacttctacc tttatatctc ttttttcttt tatgtttttt tcaagattta ctactaaaac
    24961 atcatgctta aattctagag taacaccttg aggaatattt gcttttaaat atacttcctt
    25021 ctgtccttgt attgggtcct caatggttcc atatacatgt atatcttctt ttttaacact
    25081 ttgtaccttt gataaactac cttttatata tacatctgct gttaattttt cgtctggata
    25141 tataacaaga tttttatcct ttaaatcact catattactt atagatacag gtatatcttc
    25201 aaacccttta gtttcttcag ggtcaactac agccataaca tacatccata atgcaattgc
    25261 acttaaaaat gatattattt ttattttggt attatttttt agtctattta tcatttaaat
    25321 ataccacctt tgaaaaaact tcttgtttct gtatttgttt ttaaattact acgtaatata
    25381 ttcatcattc tttctctaga aatatctcta tataacttac ctgctttagc aattgatacg
    25441 ccacctgttt cctctgatac aatcagagtt atacagtcag acacctcact tacccctatt
    25501 cctgctctat gtctagtacc taaatcttta cttaaatcct tactttctgt caatggcaaa
    25561 aaacatgcag ctgcttttac cttagaatct ctaattacaa cagctccatc gtgcaatggt
    25621 gtatttggta taaatatatt tattaatagt tgcctagaaa tttcagcatc aataatagtt
    25681 cctgtattta ttatatcact tattctagtt tctctctcca ttatgataag agcccctatt
    25741 ttttgtctag aaagtgaata taaagcttct acaatttctt ctatatttct attgagagtt
    25801 tcttcagaag tatttacatt ctttccaaat aaattaaact ttgctcttcc tatatgttca
    25861 agtcctgctc taaattctgg ttgaaaaatt ataagagcag ctataactcc ataatctaaa
    25921 gccttaagag atatccaata aacggtatgc aatttaaagg tgttactaag ttgagttgct
    25981 aataaaagaa atattatacc tttaaacact tgttctgctc ttgtatcttt tataaacata
    26041 aatattttgt aaaaaatata cgctactata gatatgtcaa ttaaatcata tatactcatt
    26101 cttaatatta tattaaaaaa tccatttaaa aaagaattta tatctaacac agttttatcc
    26161 tcctgtttga taacttctta attatttaca ctaagtatta tactatataa atctaactct
    26221 atacaaacta tttaactttt atgtaaataa ttctagtttt atatttagag attgttaaat
    26281 aatctctaaa cacattaaaa atatcaactt aataaataca tatttcttat taaagttaat
    26341 ttttacttaa ataattcaaa ccatcaaggc gcgaatacaa aatagtactc gcgccttaat
    26401 gcaattttat gctctaaaat atcttaatat atctttgttt aatatgtatt agttattata
    26461 aattctataa tccacctatc aactataaac agatacaaaa tttaaatatt acttatcatc
    26521 aatattgttc cattagggtc atttttatat taaagtaata acttatttca tatgtagatt
    26581 agataaattt attttaatat cttactataa actatcatta aaactcatta tataactata
    26641 tagaatcttc tacaatgaaa ataatacaca atacctaaag ttatat
//
LOCUS       DAEPXF010000039        26269 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain HB27
            SAMN13376389-rid12479423.denovo.041, whole genome shotgun sequence.
ACCESSION   DAEPXF010000039 DAEPXF010000000
VERSION     DAEPXF010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13376389
            Sequence Read Archive: SRR10518432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 26269)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26269)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001069624.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001069624.1
            Genome Coverage       :: 315x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/15/2021 01:54:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,165
            CDSs (total)                      :: 4,076
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 89
            rRNAs                             :: 10, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 10 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 19 of 69
            Pseudo Genes (incomplete)         :: 44 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 10 of 69
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26269
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13376389-rid12479423.denovo.041"
                     /strain="HB27"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="China: Hebei"
                     /collection_date="2017"
     gene            635..3685
                     /locus_tag="KRL67_001426"
     CDS             635..3685
                     /locus_tag="KRL67_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SrtB-anchored collagen-binding adhesin"
                     /protein_id="HBH1522320.1"
                     /translation="MKKMLKRLCTGFLALATVVTALPTTPVHAESKQYWTESAERVGI
                     IEKVMNDGSIGSTFNEGYMKVEGETAYCIDINTNFKNGYKTRADASSRMSADQISDVA
                     LSLEYVKQYGEAHKELNYKQVYLLEQCVVWQRLSVHFGWQCDNVRASYDEIPKATQDE
                     VFSGAKAFIKENKGRYECGGYIYSGEGQELGQFWAKLNVGNAKLQKTSSNTSITDGNG
                     NYSIAGATYGVFSDKDCTKQLATLTTDENGNTDVVEVKAGTVYIKELSAPAGYKVDKT
                     IYSLKVEVGKTAILKVSDTPKVTDTLIELFKIDMETQKDNPQGNASLAGAEFTWKYYA
                     GFYNKDNLPAEATRTWVTKTIAETDSDGTTHYIAKLADAYKVSGDSFYMQDGKAVLPL
                     GTLTVEETKAPNGYLLDGAYMQAGDKSEQIKGLYVTQITEDGDLAVLTGSNQFSVSDK
                     VIRGGVKIQKRDLETGDTKPQGSATLKDTAFDIISLNDNAVLVEGKLYKKNEVVKTIH
                     TDIEGVASTSSDLLPYGKFRIVESEAPDGYLTDGAKPIDFTITENGKIVDLTDEAHSI
                     YNQIKRGDIEGVKIGVGTHKRLADVPFRITSKTTGENHVVVTDDNGQFSTSADWASHK
                     HNTNAGKTSEDGVWFGTSEPDDSKGALPYDTYIIEELRSDSNKGFELIPPFEIVVSRN
                     NLVIDLGTLTDEYEKEISIHTTATSKDGEKTILAGKEITIVDTVKLDGLTKGTKYQLK
                     GWQMLKEENAELLIDGKRVENDYTFVADDEEMKVEISYTFNASALGGKNLVTFEELYD
                     LSNPDEPVKVAEHKDIEDDGQTVLITERIVKIHTTATDKDGNKELEAGKDVTIIDTVI
                     LEGLEVGTQYKLVGWQMLKEENAELLINGKRVKSDYTFTADSETMKVEVAFTFDATSL
                     DGKQLVTFEELYDLSNPDEPKKVTEHKDIEDKGQTITFKEKTKVPEEPKKPETPQTPD
                     TPHKTDSPKTGDSTNLYGLLALLLTSGAGLAGIFFCKRRKMKKS"
     gene            3922..4248
                     /locus_tag="KRL67_001427"
     CDS             3922..4248
                     /locus_tag="KRL67_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HBH1522321.1"
                     /translation="MEMKYVVPDMAQSFGTLEFAGESEPVFERDKNNRRVLARRSYNL
                     YSDVQKGENVVVEIPVQAGEKHFKYEQKVKLVNPKLYGRGYAIGDMGHTDYVLLADDI
                     VAVEEK"
     gene            4264..4647
                     /locus_tag="KRL67_001428"
     CDS             4264..4647
                     /locus_tag="KRL67_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009588269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HBH1522322.1"
                     /translation="MRLSNGFVIDKEKTFGELKFTAVRDVFLQNEDGTPSTQLKKRIY
                     DLKCSLHGGIIPVSVPPEVPLREFPYNAVVELVNPVADTVSRKTYTGADVDWYVKAED
                     IVLKNKSNQNAGNTQNHTPQGQPKK"
     gene            4749..5513
                     /locus_tag="KRL67_001429"
     CDS             4749..5513
                     /locus_tag="KRL67_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008687118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HBH1522323.1"
                     /translation="MDSWFTVEQIDQDTFVISEYKHWEETHCYLLCGTKRAILIDTGL
                     GVSNIREVIDGLTKLPVTVITTHVHWDHIGGHKYFRSIAVHEAEKEWLSVKFPIPLQV
                     VKNNIMCNPCDFPSDFKVEKYQIFQGVPQMILHDEDCIDLGNRKLVVIHTPGHSPGHC
                     CFYEADRKYLYSGDLIYSGCLDAFYPSTNPQEFWKSVRKIQSLKISRILPGHHHLSIP
                     VTIIDKIETAFHNLSNEGKLKQGNGIFSYEGFQIHI"
     gene            5612..7006
                     /locus_tag="KRL67_001430"
     CDS             5612..7006
                     /locus_tag="KRL67_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008787614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBH1522324.1"
                     /translation="MRMIWNKGHRIRASDKHLVYHFSIGTLLFVFVAVLLLLNSKQLM
                     CTDWEHFSLLENGLTLSPYNFITILIATGVCALVAFLYYRFCYDSFKKLLHRQKLARM
                     VLENKWYEADTVQDSIFFTDLQSRSREKIVWFPKIYYQMEKGLLHIRCEITLGKYQDQ
                     LLRLEDKLESGLYCELTDKTLHDGYIEYTLLYDMIANRITIDEVRAENGCLRLMKNLV
                     WEYDALPHALIAGGTGGGKTYFLLTLIEALLHTNAVLYILDPKNADLADLGTVMGNVY
                     HTKEEMIDCVNAFYEGMVQRSEEMKRHPDYKTGENYAYLGLPPCFLIFDEYVAFFEML
                     GTKESVSLLSQLKKIVMLGRQAGYFLIVACQRPDAKYFSDGIRDNFNFRVGLGRISEL
                     GYGMLFGSDVKKQFFQKRIKGRGYCDVGTSVISEFYTPLVPKGHDFLQTIGPLAQARQ
                     DVTATCEAKGDGTD"
     gene            6976..7161
                     /locus_tag="KRL67_001431"
                     /pseudo
     CDS             6976..7161
                     /locus_tag="KRL67_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002907732.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
     gene            7184..8389
                     /locus_tag="KRL67_001432"
     CDS             7184..8389
                     /locus_tag="KRL67_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000398284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HBH1522325.1"
                     /translation="MEGFLLNEQTWLQHLKEKRLAYGLSQNRLAVATGITRQYLSDIE
                     TGKVKPSEDLQQSLWEALERFNPDAPLEMLFDYVRIRFPTTDVQQVVENILQLKLSYF
                     LHEDYGFYSYSEHYALGDIFVLCSHELDKGVLVELKGRGCRQFESYLLAQQRSWYEFF
                     MDVLVAGGVMKRLDLAINDKTGILNIPVLTEKCQQEECISVFRSFKSYRSGELVRKEE
                     KECMGNTLYIGSLQSEVYFCIYEKDYEQYKKNDIPIEDAEVKNRFEIRLKNERAYYAV
                     RDLLVYDNPEHTAFKIINRYIRFVDKDDSKPRSDWKLNEEWAWFIGNNRERLKLTTKP
                     EPYSFQRTLNWLSHQVAPTLKVAIKLDEINQTQVVKDILDHAKLTDRHKQILKQQSVK
                     EQDVITTKK"
     gene            8432..8653
                     /locus_tag="KRL67_001433"
     CDS             8432..8653
                     /locus_tag="KRL67_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002570387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH1522326.1"
                     /translation="MNFGQNLYNWFLSNAQSLVLLAIVVIGLYLGFKREFSKLIGFLI
                     IAIIAVGLVFNAAGVKDILLELFNRIIGA"
     gene            8770..9267
                     /locus_tag="KRL67_001434"
     CDS             8770..9267
                     /locus_tag="KRL67_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000342537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HBH1522327.1"
                     /translation="MDDMQVYIANLGKYNEGELVGAWFTFPIDFEEVKEKIGLNDEYE
                     EYAIHDYELPFTVDEYTSIGELNRLWEMVSELPEELQSELSALLTHFSSIEELSEHQE
                     DIIIHSDCDDMYDVARYYIEETGALGEVPASLQNYIDYQAYGRDLDLSGTFISTNHGI
                     FEIVY"
     gene            9242..9748
                     /locus_tag="KRL67_001435"
     CDS             9242..9748
                     /locus_tag="KRL67_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016897981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HBH1522328.1"
                     /translation="MGFLKSSIKSVGTLLLADFLFYGVAQSATPIFYERIDYMKKIRS
                     YTSIWSVEKVLYSINDFRLPFPITFTQMTWFVVSLFAVMILGNLPPLSMIEGAFLKYF
                     GIPVAFTWFMSTKTFDGKKPYGFLKSVIAYALRPKLTYAGKKVTLGRNQPQEAITAVR
                     SEFYGISN"
     gene            9732..12179
                     /locus_tag="KRL67_001436"
     CDS             9732..12179
                     /locus_tag="KRL67_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBH1522329.1"
                     /translation="MAYPIKYIENNLVWNKDGECYAYYELVPYNYSFLSPEQKIQVHD
                     SFRQLIAQNRDGKIHALQISTESSIRSAQERSKNEVTGKLKAVAYDKIDQQTDALISM
                     IGENQVNYRFFIGFKLLLNDQEFSMKSLTVEAKNALSDFVYDVNHKLMGDFVSMSNDE
                     ILRFQKMEKLLENKISRRFKIRRLDKDDFGYLIEHLYGQTGTAYEEYEYHLSKKKLDN
                     ETLIKYYDLIKPTRCLVEEKQRYLKIQQEDETVYVAYFTINSIVGELDFPSSEIFYYQ
                     QQQFTFPIDTSMNVEIVANRKALSTVRNKKKELKDLDNHAWQSDNETSSNVAEALESV
                     NELETNLDQSKESMYKLSYVVRVSANDLDELKRRCNEVKDFYDDLSVKLVRPFGDMLG
                     LHEEFLPASKRYMNDYIQYVTSDFLAGLGFGATQMLGENEGIYVGYSLDTGRNVYLKP
                     ALASQGVKGSVTNALASAFVGSLGGGKSFANNLIVYYAVLYGAQAVIVDPKAERGRWK
                     ETLPEISHEINIVTLTSDEKNKGLLDPYVIMKNPKDSESLAIDILTFLTGISSRDGER
                     FPILRKAIRAVTNSEVRGLMKVIEELRVENTPLSTSIADHIESFTDYDFAHLLFSNGY
                     VEQSISLEKQLNIIQVADLVLPDKETSFEEYTTMELLSVAMLIVISTFALDFIHTDRS
                     IFKIVDLDEAWSFLQVAQGKTLSMKLVRAGRAMNAGVYFVTQNTDDLLDEKLKNNLGL
                     KFAFRSTDLNEIKKTLAFFGVDPEDENNQKRLRDLENGQCLISDLYGRVGVIQFHPVF
                     EELLHAFDTRPPVRKEV"
     gene            12182..14359
                     /locus_tag="KRL67_001437"
     CDS             12182..14359
                     /locus_tag="KRL67_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HBH1522330.1"
                     /translation="MKPSIVNRIKSNWTLKRLGKVAMTVAFTLVIAIFLLAMLGTVVQ
                     AAGLVDDTVNVANEYSRYPLENYQLDFYVDNSWGWLPWNWSDGIGKQVMYGLYAITNF
                     IWTISLYVSNATGYLVQEAYSLDFISATADSIGKNMQTLAGVSANGFSTEGFYVGFLL
                     LLILVLGVYVAYTGLIKRETTKAIHAIMNFVLVFILSASFIAYAPDYIKKINDFSSDI
                     SNASLSLGTKIVMPHSDSQGKDSVDLIRDSLFSIQVQQPWLLLQYNSSDIESIGIDRV
                     ESLLSTSPDSNNGEDREKIVAEEIEDRSNTNLTITKTINRLGTVFFLFVFNIGISIFV
                     FLLTGIMIFSQVLFIIYAMFLPVSFILSMIPSFDGMSKRAITKLFNTILTRAGITLII
                     TTAFSISTMLYTLSAGYPFFLIAFLQIVTFAGIYFKLGDLMSMFSLQSNDSQSVGSRV
                     MRKPRMLMHAHMHRLQRKLGRSMTTLGAGSAIVTGKKGQSGSGSSARTQADHSRPDGK
                     EKSTLGKRIGQTIGTVADTKDRMVDTASGLKEQVKDLPTNARYAVYQGKSKVKENVRD
                     LTSSISQTKADRASGRKEQQEQRRKTIAKRRSEMEQVKQKKQPASSVHERPTTRQEQY
                     HDEQTSKQSNIQTSYKESQQAKQERPAVKSDFSSPKVERQGNTVQEKTVQKPATSTTT
                     ADRTSQRPITKERPSTVQRVPLQNTRSRPPIKTATIKKVGKKP"
     gene            14356..15357
                     /locus_tag="KRL67_001438"
     CDS             14356..15357
                     /locus_tag="KRL67_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018368130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysozyme/C40 family peptidase"
                     /protein_id="HBH1522331.1"
                     /translation="MKLKTLVIGGSGLFLMVFSLLLFVAILFSDEQDSGISNIHYGGV
                     NVSAEVLAHKPMVEKYAKEYGVEEYVNILLAIIQVESGGTAEDVMQSSESLGLPPNSL
                     STEESIKQGVKYFSELLASSERLSVDLESVIQSYNYGGGFLGYVANRGNKYTFELAQS
                     FSKEYSGGEKVSYPNPIAIPINGGWRYNYGNMFYVQLVTQYLVTTEFDDDTVQAIMDE
                     ALKYEGWRYVYGGASPTTSFDCSGLTQWTYGKAGINLPRTAQQQYDVTQHIPLSEAQA
                     GDLVFFHSTYNAGSYITHVGIYLGNNRMFHAGDPIGYADLTSPYWQQHLVGAGRIKQ"
     gene            15354..16289
                     /locus_tag="KRL67_001439"
     CDS             15354..16289
                     /locus_tag="KRL67_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002989471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HBH1522332.1"
                     /translation="MRKEDLMMKFRKNQNKEKQIPKEKKPRVYYKVNPHKKVVIALWV
                     LLGLSFSFAIFKHFTAIDTHTIHETTIIEKEYVDTHHVENFVENFAKVYYSWEQSDKS
                     IDNRMESLKGYLTDELQALNVDTVRKDIPVSSSVRGFQIWTVEPTGDNEFNVTYSVDQ
                     LITEGENTKTVHSAYIVSVYVDGSGNMVLVKNPTITNIPKKSSYKPKAIESEGTVDSI
                     TTNEINEFLTTFFKLYPTATASELSYYVNDGILKPIGKEYIFQELVNPIHNRKDNQVT
                     VSLTVEYIDQQTKATQVSQFDLVLEKNGSNWKIIE"
     gene            16532..16648
                     /locus_tag="KRL67_001440"
     CDS             16532..16648
                     /locus_tag="KRL67_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance determinant leader
                     peptide"
                     /protein_id="HBH1522333.1"
                     /translation="MILKYPENICMLCMPMVMHKNPSDKSIYHWDFYALLGF"
     gene            16664..18583
                     /gene="tet(M)"
                     /locus_tag="KRL67_001441"
     CDS             16664..18583
                     /gene="tet(M)"
                     /locus_tag="KRL67_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011117014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance ribosomal protection
                     protein Tet(M)"
                     /protein_id="HBH1522334.1"
                     /translation="MKIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTD
                     NTLLERQRGITIQTGITSFQWENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAK
                     DGVQAQTRILFHALRKMGIPTIFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVEL
                     YPNMCVTNFTESEQWDTVIEGNDDLLEKYMSGKSLEALELEQEESIRFQNCSLFPLYH
                     GSAKSNIGIDNLIEVITNKFYSSTHRGPSELCGNVFKIEYTKKRQRLAYIRLYSGVLH
                     LRDSVRVSEKEKIKVTEMYTSINGELCKIDRAYSGEIVILQNEFLKLNSVLGDTKLLP
                     QRKKIENPHPLLQTTVEPSKPEQREMLLDALLEISDSDPLLRYYVDSTTHEIILSFLG
                     KVQMEVISALLQEKYHVEIELKEPTVIYMERPLKNAEYTIHIEVPPNPFWASIGLSVS
                     PLPLGSGMQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYS
                     PVSTPADFRMLAPIVLEQVLKKAGTELLEPYLSFKIYAPQEYLSRAYNDAPKYCANIV
                     DTQLKNNEVILSGEIPARCIQEYRSDLTFFTNGRSVCLTELKGYHVTTGEPVCQPRRP
                     NSRIDKVRYMFNKIT"
     gene            18702..18869
                     /locus_tag="KRL67_001442"
     CDS             18702..18869
                     /locus_tag="KRL67_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich KTR domain-containing protein"
                     /protein_id="HBH1522335.1"
                     /translation="MCPVCGNKTRLKIREDTELKKFPLYCPKCRQENLIEIKQFKVTV
                     ITEPDAKTQSR"
     gene            complement(18929..19282)
                     /locus_tag="KRL67_001443"
     CDS             complement(18929..19282)
                     /locus_tag="KRL67_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBH1522336.1"
                     /translation="MRKKEDKYDFRAFGLAIKEARLKRGLTREQVGALIEIDPRYLTN
                     IENKGQHPSIQVLYDLVSLLHVSVDEFFLPANNLVKSTRRLQIEKYMDSFTDKELSLM
                     ESLASGINEARNIED"
     gene            19787..20209
                     /locus_tag="KRL67_001444"
     CDS             19787..20209
                     /locus_tag="KRL67_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006733729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HBH1522337.1"
                     /translation="MKPSSFQTTIENQFDYICKRAMEDERKNYMLYLSRIAKREVSFS
                     DVGDYLVSQFATTDNYSTDFQIFTLNGLSVGVENDLLSEALRELPDKKREILLLFYFM
                     DMSDSEIADLLKLNRSTVYRHRTSGLALIKKFMEEFEE"
     gene            20206..20436
                     /locus_tag="KRL67_001445"
     CDS             20206..20436
                     /locus_tag="KRL67_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002411750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBH1522338.1"
                     /translation="MKTQYPMIPFPLIVKATDGDTEAINQILHHYRGYITKRSLRLMK
                     DEYGNQSMVVDEVLRGRMETRLITKILSFEIK"
     gene            20897..21100
                     /locus_tag="KRL67_001446"
     CDS             20897..21100
                     /locus_tag="KRL67_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006730246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HBH1522339.1"
                     /translation="MKQTDIPIWERYTLTIEEASKYFRIGENKLRRLAEENKNANWLI
                     MNGNRIQIKRKQFEKIIDTLDAI"
     gene            21182..22399
                     /locus_tag="KRL67_001447"
     CDS             21182..22399
                     /locus_tag="KRL67_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020220015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HBH1522340.1"
                     /translation="MSEKRRDNKGRILKTGESQRKDGRYLYKYIDSFGEPQFVYSWKL
                     VATDRVPAGKRDCISLREKIAELQKDIHDGIDVVGKKMTLCQLYAKQNAQRPKVRKNT
                     ETGRKYLMDILKKDKLGVRSIDSIKPSDAKEWAIRMSENGYAYQTINNYKRSLKASFY
                     IAIQDDCVRKNPFDFQLKAVLDDDTVPKTVLTEEQEEKLLAFAKADKTYSKNYDEILI
                     LLKTGLRISEFGGLTLPDLDFENRLVNIDHQLLRDTEIGYYIETPKTKSGERQVPMVE
                     EAYQAFKRVLANRKNDKRVEIDGYSDFLFLNRKNYPKVASDYNGMMKGLVKKYNKYNE
                     DKLPHITPHSLRHTFCTNYANAGMNPKALQYIMGHANIAMTLNYYAHATFDSAMAEMK
                     RLNKEKQQERLVA"
     gene            22579..24345
                     /locus_tag="KRL67_001448"
                     /pseudo
     CDS             22579..24345
                     /locus_tag="KRL67_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860734.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-like protein"
     gene            complement(24580..26178)
                     /locus_tag="KRL67_001449"
     CDS             complement(24580..26178)
                     /locus_tag="KRL67_001449"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative manganese-dependent inorganic
                     diphosphatase"
                     /protein_id="HBH1522341.1"
                     /translation="MSLLVFGHKNPDTDSICSAISLAHLKNKLGIKATAYALGDIRKE
                     AKYALDYFKVDAPEILDNVRIQVRDLNYDKVVPLTPTSSILEAYNLMDEKNVKTLPVT
                     YDDGTFAGIITMKEIAKNLLHQHFTTIHTSLSNICKNLNGTILVDCGEDVKGRVVTLS
                     FGMETLIETLKEGDIAIVGDRYDSIEYAIDTKVKLLILTNHTEISKDLLALAKINGVS
                     VVSVESDNYKTSNVINQCSFLDSIEATENLITFKEDDYMDEIKEIMLETNFRSYPILD
                     DDNKFLGLVSKGHLLNPSKKNVVLVDHNEYAQSADGIEQANIVEIIDHHKLGGISTDV
                     PMSFRVMPVGCNSTIIYQMYKENNVEIPYEIAGLLLSAILSDTLLFKSPTTTDMDKKA
                     CEELSKIAKVDMEKYAMDMFKCGTSLDEYTIEEIVNMDFKEFNMGGHRVGIGQVFTLD
                     IDSIFAKKDEFLSYINSTDYDKLVLAVTDIIKEGSYLIYKAEDSLIANAFGIEGVQGT
                     FAPGVVSRKKQLVPNLTTAIKNFK"
BASE COUNT         8736 a         4644 c         5447 g         7442 t
ORIGIN      
        1 tttataactt tggtttacaa taatagagac aataaatcct aaaaaacata ataagaaaaa
       61 agtgcttaat attttagaaa gactggttta gtttacacca gctaagccag tcaaggaata
      121 atttaaagtg taatatataa taatgaaaaa gaacatgcta ttagaagttt ctaatgacat
      181 gttctttttt tgtagaaatc agcattatta tataaaaatt taagactgta aattttaatt
      241 gtgttgtaac taagtatgta ttttaaacat agtataatat agacccaaaa tggagggatt
      301 ataaatgaga aatattatac tttatttaaa tgatgatact tttatatcta aaaacacaat
      361 aaaaatctga ttatccgcta aaacacagta tctatcagca ttttcaagaa aacaaataca
      421 tcaatttact acttatttac tactaacgaa tgagccttga tacgcaagtt ttcctagata
      481 tgcaaagata tggtacagca catcagtatt aaaataagaa aaaattgaat aactcataga
      541 ctatggaacg cctaacatga cgttcctttt ctatttccag ccgttcttat tggacggcta
      601 ttttattacc caaaatgaaa ggagcattag atttatgaaa aagatgttaa aacgattgtg
      661 tacgggcttc ttagctcttg caactgtcgt tactgcttta ccgactacac ccgtccatgc
      721 agaaagtaag caatactgga cggaaagtgc agagcgtgtc ggtatcattg aaaaagtaat
      781 gaatgacggt tctatcggtt cgacattcaa tgagggctat atgaaagtcg agggcgaaac
      841 tgcctattgt atcgacatta acacaaactt taagaacggt tacaagacca gagctgacgc
      901 aagctcacgc atgagtgctg accagatttc agatgtggcg ttatccttag agtatgtcaa
      961 acagtatggc gaagctcaca aggaactgaa ctacaaacaa gtctatctgt tggaacaatg
     1021 tgtagtctgg cagagattga gcgtacattt tggctggcaa tgtgataacg tgcgagcttc
     1081 ttatgatgaa attccaaagg caacacagga cgaagttttc tctggtgcaa aagcctttat
     1141 caaagaaaat aaaggacgct atgaatgtgg cggttatatc tactctggcg aggggcagga
     1201 attgggacaa ttctgggcga agctgaatgt cggaaatgca aaactgcaaa agacttccag
     1261 taataccagc attaccgacg gtaacgggaa ttactctatt gcaggtgcaa cctatggtgt
     1321 cttttctgat aaggactgta cgaaacagct tgccaccctt acgactgatg aaaacggaaa
     1381 tacagatgtt gtagaggtaa aggctggcac agtttatatc aaggagttat ccgcaccagc
     1441 aggatataaa gtagataaaa ccatatattc cttaaaggtt gaagttggaa agacagcaat
     1501 tttgaaagta tcagatacgc caaaagtaac ggacactttg attgagcttt tcaagattga
     1561 tatggaaaca cagaaagaca atccgcaagg gaacgcttct ttagcaggtg cggaatttac
     1621 atggaagtat tatgcaggct tctataataa agacaatctc cctgccgaag ctactcgtac
     1681 atgggttaca aagacaatcg ctgaaacaga cagcgacggg acaactcact acatcgcaaa
     1741 attagcggac gcatacaagg tatctggcga tagcttctat atgcaggacg gcaaggcggt
     1801 tcttccactt ggaacgctaa ccgttgagga aacgaaagct ccaaacggct acttgttaga
     1861 cggtgcatat atgcaggctg gcgataagtc cgaacagata aaaggcttat atgtaacaca
     1921 gattacagag gacggcgacc ttgccgtact tactggaagt aaccagtttt ctgtatcaga
     1981 caaggttatc cgtggtggtg tgaaaattca gaaacgagat ttagaaacgg gcgatacaaa
     2041 gccacaagga agtgctacct tgaaagatac tgcttttgac atcatttcct taaatgataa
     2101 tgcggtattg gttgagggca aattatacaa gaaaaatgaa gtggtaaaaa caatccatac
     2161 cgatattgag ggtgtcgctt ctacttcttc tgacctttta ccttatggaa aattccgtat
     2221 cgttgaaagt gaagctcccg acggttactt aactgacggt gcaaagccga ttgattttac
     2281 aatcacagaa aatggaaaaa tcgtggactt aaccgacgaa gctcattcta tctataatca
     2341 gattaagcgt ggagatattg agggtgtaaa aatcggtgta ggtacacaca agcgtcttgc
     2401 tgatgttccg tttcggatca caagcaagac gacgggcgaa aatcatgtag tggtaactga
     2461 tgataacggg caattctcca cttctgctga ctgggcttct cataagcaca ataccaacgc
     2521 aggcaagacc agcgaggacg gtgtgtggtt tggaacttct gaaccagacg acagcaaggg
     2581 tgcattacct tatgatacct acatcattga agaattacgc tctgatagta acaaaggctt
     2641 tgaacttatc ccaccttttg aaatcgtggt atcaagaaat aaccttgtga ttgatttagg
     2701 aacgctgact gatgaatacg aaaaagaaat ttctatccat accacagcga ccagcaagga
     2761 cggcgaaaaa actatccttg caggaaaaga gattacgatt gttgatactg taaaattgga
     2821 cggacttaca aaaggcacaa aatatcagct caaaggctgg cagatgttaa aggaagaaaa
     2881 cgcagaactt cttattgacg ggaaacgtgt agaaaacgat tatacctttg tcgctgatga
     2941 tgaggaaatg aaagtggaaa tttcctatac attcaatgcg tctgctttag gtggcaaaaa
     3001 ccttgttacc tttgaagaat tgtatgattt aagcaatcca gacgaacccg taaaagttgc
     3061 ggaacataaa gacattgagg acgacggaca gacggtactt atcacagagc gtatcgtcaa
     3121 aattcatacg actgctacgg ataaggacgg caacaaagag cttgaagctg gaaaagacgt
     3181 tacaatcatt gataccgtaa tcttagaggg cttagaagtc ggtacacagt acaaacttgt
     3241 gggctggcag atgttgaaag aagaaaatgc agaacttctt atcaatggaa aacgtgtgaa
     3301 aagtgattac acttttactg ctgacagcga aactatgaaa gtggaagttg cctttacctt
     3361 tgacgctact tctcttgacg gcaaacagct tgtaactttt gaagaattgt acgatttaag
     3421 caatccagac gagccgaaga aagttaccga gcataaggat attgaggata agggacagac
     3481 gattaccttt aaggaaaaaa ccaaagtccc agaagaacca aagaaacccg aaacaccaca
     3541 gacaccagat acaccgcata aaacagacag tccaaaaact ggcgatagca caaaccttta
     3601 cggactgctt gcacttcttt tgacttctgg tgctggactg gcaggtatct tcttctgcaa
     3661 acgccgtaaa atgaagaaat cataaggcgg taacggtatg agaaaagaaa aatcacgttc
     3721 tccgccgata ccgtgtcggc agacttccgc ttattctgga ttgtctgcta cttacctgca
     3781 agaacacgga tagtcaaggg tgcgtaagca ttgattttac cctttactat ctgggggatt
     3841 gctggtactt cccaaaccgc cagaataaga aatcacaatc aaaaaactta tcaaaaatag
     3901 aaagaaatga ggtaaaacac tatggaaatg aaatatgtcg tgccagatat ggcacagtct
     3961 tttggaactc ttgaatttgc aggcgaaagt gagccagtct ttgaaagaga taaaaataac
     4021 cgcagggtct tagccagacg aagctataac ctttattctg acgtacaaaa aggcgaaaat
     4081 gttgtggtag aaattcccgt gcaggctggc gaaaagcatt tcaagtatga acagaaagta
     4141 aaacttgtca atccgaagtt atatggcaga ggttacgcaa tcggggatat gggacatacc
     4201 gattatgtgt tgcttgctga tgatattgta gcagttgaag aaaagtaaag gagtgaagaa
     4261 tttatgagat tatcaaacgg gtttgttatt gacaaagaga aaacatttgg agaattaaaa
     4321 tttacagcgg tgcgagatgt gttcttgcag aatgaggacg ggacaccgag tacccagctt
     4381 aaaaagcgta tctatgattt gaagtgcagt ctgcatggtg gaattatccc cgtgtccgta
     4441 ccgccagaag taccgttaag ggaatttccg tacaatgcgg ttgtggagct tgtcaatcca
     4501 gtagccgata cggtatcaag aaaaacctat acgggtgcag atgtggactg gtatgtaaag
     4561 gcagaggata ttgtattgaa gaataaaagc aaccagaacg caggcaatac acagaaccat
     4621 acaccgcagg gacaaccgaa aaaatagact tgctgaatag attttcgttg tagaggatat
     4681 acggaactgt tataatatgg gtatgaagaa agcgacaaat taaaattttt agggagtgag
     4741 aatagtatat ggacagttgg tttaccgtcg aacaaataga ccaagataca ttcgtcatca
     4801 gcgaatacaa acactgggaa gaaacacatt gttatctatt gtgtgggaca aaaagagcca
     4861 ttctgattga tacaggttta ggtgtttcca acatcagaga ggttattgat ggattgacga
     4921 aactgcccgt tacagtaatc actactcatg ttcattggga tcatatcggt ggacataaat
     4981 attttcggtc tattgcagtt catgaagcag aaaaagaatg gctatcggtc aaattcccta
     5041 tacctttgca agtggtaaaa aacaatatta tgtgcaatcc atgtgatttc ccctcggatt
     5101 ttaaggtaga gaaatatcag atttttcaag gggttccgca aatgatttta catgatgaag
     5161 attgtattga cttagggaac agaaagttgg tagttattca cacgcccggt cattctcccg
     5221 gacattgttg tttctatgaa gcggatagaa aatatctgta ttccggcgat ttgatatata
     5281 gcggttgcct tgatgctttt tatccatcaa caaacccaca agaattttgg aagtctgtta
     5341 gaaaaattca atctttaaag ataagtcgaa ttttgccagg acatcatcat ttatctattc
     5401 cagttactat aattgacaag atagagacag cttttcacaa cttgtcaaat gaagggaaat
     5461 taaaacaagg gaatggtata ttcagctatg aggggtttca aatccatatc tgaaatgcaa
     5521 tcttagtttt acaattttat atccatacac acaaagcagt tagtcttagg attaactgtt
     5581 tttttcatac ccagaaagga gtgattgatt tatgcgtatg atttggaaca aaggacaccg
     5641 tatcagagcc agcgacaagc accttgtcta ccacttttcc atagggacgc ttctgtttgt
     5701 gttcgtggcg gttctcctgc tactgaatag caaacagctc atgtgtaccg attgggagca
     5761 tttcagcttg ttagagaacg gcttgacgct ttccccttac aacttcataa ccatactgat
     5821 agcgactggt gtttgtgcat tggtcgcttt tctgtattac cgcttctgtt atgacagttt
     5881 caagaagctc ctgcaccgtc aaaagctggc aagaatggta ctggaaaata agtggtatga
     5941 agctgatacc gtacaagaca gcattttttt tactgacctg caaagcagat caagggaaaa
     6001 aatcgtctgg tttccaaaga tttattatca aatggaaaag ggactgcttc atatccgctg
     6061 tgaaattacg ctggggaaat atcaagacca gcttttacgg ttagaggata aattggaaag
     6121 tggcttgtat tgtgagctga ccgacaagac cctgcatgac ggctatatcg aatataccct
     6181 gctttatgat atgatagcaa accgcattac cattgatgaa gtacgggcag aaaacggctg
     6241 tcttagactg atgaaaaatc ttgtgtggga atatgacgca ctccctcacg cccttatcgc
     6301 tggtggaact ggtggcggta aaacatactt tttgctgaca ctcattgaag ccttactgca
     6361 taccaacgct gtcctttaca tcttagaccc gaaaaatgcg gacttagcag acttagggac
     6421 agttatggga aatgtgtatc ataccaaaga agaaatgatt gattgcgtca atgcctttta
     6481 tgagggaatg gtacagcgaa gcgaggaaat gaagcgacac ccagattata agacgggcga
     6541 aaactacgcc tatctgggac tgccaccctg ctttcttatc tttgatgaat atgtggcgtt
     6601 ttttgaaatg ctggggacaa aagaaagcgt gagcttactt agccagttaa agaaaatcgt
     6661 catgctgggg cgacaagcag gttattttct tattgttgcc tgccagcgtc cagacgcaaa
     6721 gtatttctcg gacggtatca gagataactt caatttccgt gtgggacttg ggcgtatcag
     6781 cgaattaggt tatggtatgc tgttcggttc ggatgtgaaa aagcagtttt tccagaagcg
     6841 tatcaaaggg cgtggctatt gtgatgtagg gacaagtgtt atcagcgaat tttacacgcc
     6901 tttagtcccg aaaggacatg attttttgca gactatcggc cctcttgcac aagcaaggca
     6961 ggacgtgacg gcgacgtgcg aagcgaaagg cgacggcacg gactagccgt tgctggtgtg
     7021 gcgtaagcca cgccagcagg taaaacccct cggtatctaa caggggggta caaatcgaca
     7081 ggaaacagtc aaaaaaacat tagaaaatcc tttggttaca agggatttac aaaatttcag
     7141 cgtatgtcaa atgggcttta aaagttgaca tacgcctttt tgattggagg gatttttact
     7201 gaatgaacaa acttggttac agcatttaaa agaaaaacgc ttggcttatg gactatctca
     7261 aaaccgttta gctgttgcga ctggtattac aaggcagtat ctaagcgata ttgaaacagg
     7321 aaaagtcaag ccatcagagg atttacagca gtccctttgg gaagctctgg aacgcttcaa
     7381 tcccgacgct ccccttgaaa tgctgtttga ttatgtaagg attcgctttc cgacaacaga
     7441 cgtacagcag gtggtcgaaa acatcttaca actgaaactg tcctattttc ttcatgagga
     7501 ctatggtttc tattcttatt cagagcatta tgctttaggc gacatattcg tcctttgctc
     7561 ccatgaactg gacaaaggag ttctggtgga attgaaaggt cgtgggtgca gacaatttga
     7621 aagctatctt ctggcacaac aaagaagctg gtatgagttc tttatggacg ttttggtggc
     7681 tggcggtgtg atgaaacgcc ttgaccttgc cattaacgat aagacaggga ttttgaatat
     7741 ccctgtactc actgaaaagt gccaacagga agaatgtatc tccgtcttcc gcagttttaa
     7801 aagctatcgc agtggcgaac tggtacgcaa agaggaaaag gaatgtatgg gaaacaccct
     7861 ctatatcggt tcattacaaa gtgaagttta tttctgtatc tatgaaaagg actacgagca
     7921 gtacaagaaa aatgatattc ccattgaaga cgcagaagta aaaaaccgtt ttgagattcg
     7981 attgaaaaat gagcgtgcct attatgcagt ccgtgattta ctcgtctatg acaatccaga
     8041 gcataccgcc tttaaaatta tcaatcggta tatccgtttt gtagataaag acgattccaa
     8101 acctcgttct gattggaaac tgaatgaaga atgggcttgg tttattggga acaatcgtga
     8161 acgattaaaa ctaaccacaa aaccagagcc ttactccttc caaaggacgc tgaactggct
     8221 atctcatcaa gttgccccga ccttaaaggt tgcgattaaa cttgatgaaa tcaaccagac
     8281 gcaggttgta aaagacattc tcgaccatgc gaaactgaca gaccgacaca agcagatttt
     8341 gaagcaacag tcagtaaaag aacaggacgt gataacaaca aaaaaataac tcaaatacaa
     8401 attcattgaa tatagagagg agaacatttt tatgaatttt ggacaaaacc tttataactg
     8461 gtttctatca aacgctcaat cactggtgct tttagcaatc gttgtgattg gcttgtatct
     8521 tggcttcaag cgtgagttta gcaaactgat tggcttttta attattgcga ttattgcggt
     8581 tggcttagtc ttcaacgctg ctggagtaaa agacatttta ctagagctat tcaatcgcat
     8641 tattggtgct taaataaaac cgttcttttg tggaatataa gtggttttct tatgttccgc
     8701 aaaggaatgg tacaccaaac gaagtgcggt agggattttt gaatctctac aaagaaagga
     8761 cgtgaatata tggacgatat gcaagtctat attgcgaatt taggcaaata caatgagggc
     8821 gaattggtcg gtgcgtggtt tacctttccc attgactttg aggaagtcaa agagaaaatc
     8881 ggcttgaatg atgaatatga ggaatacgcc attcatgact acgagttacc ctttacggtt
     8941 gacgaataca cttccattgg cgaactcaat cgactatggg aaatggtatc ggaattaccc
     9001 gaagaattac aatcggagct atctgctctg ctcactcatt tttcaagcat tgaagaacta
     9061 agcgaacatc aagaggatat tatcattcat tccgattgtg atgatatgta tgacgtggca
     9121 cgctactaca ttgaagaaac gggtgcttta ggcgaagtac cagctagtct tcaaaactat
     9181 attgattatc aagcctatgg tcgggattta gacctttcag gaacgtttat ctcaaccaat
     9241 catgggattt ttgaaatcgt ctattaaatc tgtcggtaca ttactactgg cagattttct
     9301 attttacggg gtggctcaat cagctacccc tattttttat gaaaggattg attacatgaa
     9361 gaaaatacga agctatacca gtatctggtc tgtggaaaag gtactgtatt ctatcaatga
     9421 ttttagactt ccgtttccca taacctttac gcaaatgaca tggtttgtcg tgtcactctt
     9481 tgcagtgatg atacttggca acttgccccc tctttccatg atagagggag catttctcaa
     9541 atactttggg attcctgtgg ctttcacatg gtttatgtct acaaaaactt ttgatggtaa
     9601 aaagccttat ggatttttga agtctgtcat tgcttatgca ctgcgaccaa agctgaccta
     9661 tgcaggaaaa aaagtaacgc ttggcagaaa ccagccacaa gaagccatta cagcagttag
     9721 gagtgaattt tatggcatat ccaattaaat acattgaaaa caatctcgtc tggaataaag
     9781 acggggaatg ttatgcttac tatgagcttg ttccttacaa ttactcattt ctaagtccag
     9841 aacagaaaat acaagtgcat gattctttca gacagcttat cgcacaaaat cgtgatggca
     9901 aaattcatgc tttacaaatc agtacagaat ccagcatacg ttctgcacaa gagcgttcca
     9961 aaaatgaagt cactggcaag ctcaaagcgg ttgcctatga caaaatcgac caacagacag
    10021 acgctttaat atccatgatt ggcgaaaatc aagtgaacta ccgtttcttt atcggcttta
    10081 agttgcttct caacgatcag gagttttcta tgaaaagtct taccgttgaa gcaaaaaatg
    10141 ctttgtctga ttttgtctat gatgtgaacc ataagctgat gggcgatttt gttagtatga
    10201 gtaatgatga aatcctgcgt tttcagaaga tggaaaagct cttagaaaat aaaatctctc
    10261 gtcgtttcaa aatccgcagg ttagataagg acgacttcgg ctatctgatt gaacaccttt
    10321 acggacagac aggcactgcc tatgaagagt atgagtacca tctatcaaag aaaaagctgg
    10381 ataatgaaac gctgattaaa tactatgact tgattaagcc tactcgctgt ttggtggaag
    10441 aaaaacagcg atatttgaaa atccagcagg aagatgaaac cgtctatgta gcttacttta
    10501 ccattaacag cattgtcgga gaactggact tcccgtcctc tgaaatcttc tactaccagc
    10561 aacagcaatt tacattcccg attgatacgt caatgaatgt ggaaattgta gcgaatcgta
    10621 aagccctatc tactgtccgc aataaaaaga aagaactgaa agacttggat aaccacgctt
    10681 ggcaaagtga taatgaaacc agctccaatg tggcggaagc tctggaaagt gtgaatgagc
    10741 tggaaaccaa tttagaccaa agcaaggaat ctatgtacaa gctgtcttat gtggtaaggg
    10801 tatcagcaaa tgatcttgac gaactcaaac gtcgttgtaa tgaagtgaaa gatttttatg
    10861 acgatttaag cgtaaaactg gtacgaccat ttggggatat gctcggctta catgaagaat
    10921 ttttacctgc cagcaagcgt tatatgaatg attatattca atacgtgacc tctgatttcc
    10981 tcgctggttt aggttttggt gctactcaaa tgctggggga aaatgagggg atttatgttg
    11041 gctacagctt agatactgga cgcaatgtct atctgaaacc tgctcttgcc agtcaagggg
    11101 ttaagggttc agtaaccaat gcgttagcgt cggcttttgt tggttcgctg ggtggtggta
    11161 aatcctttgc gaataacctt atcgtctatt atgcggtgct ttatggggca caagcagtga
    11221 ttgtagaccc aaaagcagaa cgtggcagat ggaaagaaac cttgccagag atttcccatg
    11281 aaatcaatat cgtcactctg acttctgatg agaaaaacaa aggcttactt gacccttatg
    11341 tgattatgaa aaatcccaaa gattctgaat cactggctat tgatattctg acattcctta
    11401 cggggatttc ctctcgtgat ggggaacgct tcccaatcct tagaaaagcc attcgtgcag
    11461 taaccaatag tgaagtacga gggttgatga aagtgattga ggaattacgg gttgagaata
    11521 cgccactaag taccagtata gccgaccata tcgaaagttt tacagactat gactttgcac
    11581 atttattatt cagtaatggt tatgtggagc agtctatcag cttagaaaaa caactgaaca
    11641 ttatacaggt tgcggacttg gtacttcccg acaaggaaac ttcctttgag gaatatacca
    11701 ctatggagct tttatccgtt gctatgctga ttgtcattag tacctttgct ttagacttta
    11761 tccatacaga ccgaagcatt ttcaagattg tagatttaga cgaagcatgg agctttttac
    11821 aggtagcaca aggaaaaaca ctatctatga agctggttcg ggctggtcgt gctatgaacg
    11881 ctggggtata tttcgtgacc caaaatacag acgacctctt agatgaaaaa ctgaaaaata
    11941 acctcggctt aaaatttgca tttcgttcca ctgaccttaa cgagattaaa aagaccttag
    12001 ccttttttgg tgtagaccca gaggacgaaa acaatcagaa gcgattgcgt gatttggaaa
    12061 acgggcaatg ccttatcagt gatttatatg gtcgtgtcgg tgtgatacag ttccaccctg
    12121 tatttgaaga actgctccat gcctttgata ccagaccacc tgtgcgaaaa gaggtgtaaa
    12181 tgtgaaacca tcaatagtaa acagaataaa atcaaactgg acgctgaaac gtctaggtaa
    12241 agtggcaatg acagtggctt tcacacttgt gattgccatt tttcttttag ccatgctggg
    12301 aacggtggtt caagctgcgg gcttggtaga tgatacggtc aatgtggcaa atgaatacag
    12361 ccgataccca cttgaaaact atcaactgga tttttatgtg gataatagct ggggctggct
    12421 tccgtggaac tggtcggacg ggattggaaa acaggtcatg tatggactat atgccattac
    12481 caattttatt tggacaatca gtttgtatgt ttccaatgcg acaggttact tagtacagga
    12541 agcctattcc ttagacttca tttccgctac agcagattcc attggtaaga atatgcagac
    12601 cttagctggt gtgagtgcaa acggattttc aacagagggt ttctatgttg gattcctctt
    12661 actcttgatt ttggttcttg gggtttatgt tgcctatacg ggactgataa agagagaaac
    12721 cacaaaggca attcatgcca ttatgaattt tgtgctggtg tttatcctat cggcttcctt
    12781 tattgcctac gctcccgact acattaaaaa aatcaatgac ttttcatcag acatcagtaa
    12841 tgccagttta tcacttggca cgaagattgt catgccccat tccgatagtc aaggcaagga
    12901 cagcgtggac ttaatcagag atagcctgtt ttccatacag gttcagcaac cgtggctact
    12961 gcttcaatac aacagttcag acattgaaag tatcggtatt gaccgtgtgg aaagcctgct
    13021 ctccaccagc ccagattcca acaatggcga agacagagaa aaaattgttg cggaagaaat
    13081 tgaagacaga agcaatacca atctaaccat tacaaagacc attaaccgtt taggtacagt
    13141 cttcttccta tttgtcttca atattgggat ttccatattt gtattcctat taacaggaat
    13201 catgattttc tcgcaggtac tttttatcat ctatgctatg tttctgcctg tgagctttat
    13261 tttaagcatg attccatcat ttgatggtat gtcaaaacga gccataacaa agctctttaa
    13321 taccattttg acacgagctg gaatcacatt gattattacg acagcattta gtatttcaac
    13381 catgctctat accttatcgg ctggttatcc gttctttttg attgcttttc tacagattgt
    13441 gacctttgca ggaatctact tcaagctggg cgatttaatg agtatgtttt ctctacagag
    13501 taacgattct caaagtgtgg gaagtcgtgt gatgagaaaa cctcgtatgc ttatgcacgc
    13561 tcacatgcac cgtctacagc ggaaacttgg acgttccatg actactctag gggctgggtc
    13621 tgccattgtt acaggtaaaa aaggacagtc gggttcgggg agttctgcaa ggacacaagc
    13681 agatcactcc cgaccagacg gaaaggaaaa atcaacactt ggaaaacgta tcggtcaaac
    13741 catcggtaca gtagctgata ccaaagacag aatggtagac actgctagtg gtttgaaaga
    13801 acaggttaaa gatttgccga ccaatgcaag atatgcagta tatcaaggaa aatccaaagt
    13861 aaaagagaat gtccgtgatt taaccagtag tatttctcaa accaaagcgg acagagccag
    13921 tggacgcaag gaacagcagg aacaaaggcg aaaaaccatt gcgaagcgtc gctctgaaat
    13981 ggaacaggtc aaacagaaaa aacagcctgc ttcttctgtt catgaaagac cgactacaag
    14041 acaagaacaa tatcatgatg aacagacctc aaaacagtct aatattcaga cttcatataa
    14101 ggaatctcaa caagccaaac aagagcgtcc agcagttaag tccgattttt caagtccaaa
    14161 agtggaacgc caaggcaata ccgttcaaga aaaaaccgtt caaaagccag caacttcaac
    14221 cactacagca gatagaactt cacaacgtcc aatcacaaaa gaacgtccgt ctactgttca
    14281 aagagtacca ctacaaaata caagaagtag accaccaatc aaaaccgcca ccattaagaa
    14341 agtcggtaag aaaccatgaa gttgaaaact ttagtgattg gtggttctgg attattcttg
    14401 atggtcttct cactgcttct gtttgttgcc attttatttt cagatgaaca ggacagcgga
    14461 atttccaata ttcattatgg aggtgtgaat gtttccgcag aagtgctggc tcataagcct
    14521 atggtagaaa aatatgccaa agaatatggc gttgaagaat atgtcaacat acttcttgcg
    14581 attatacagg tggaatcggg cggtactgcg gaagatgtta tgcagtcctc ggaatccctc
    14641 ggtcttccac ctaattcatt gagtacagaa gaatccatta agcaaggtgt gaagtatttc
    14701 agtgaattat tagccagtag cgaaaggctc agtgtagatt tagaatcggt tatccagtcc
    14761 tacaattatg gtggtggttt cttagggtat gtggctaatc gtggaaataa atataccttt
    14821 gaactggctc aaagtttctc aaaagagtat tcaggtggcg aaaaagtgtc ttaccccaat
    14881 cccatagcca tacctatcaa tgggggctgg cgatacaact atggcaatat gttttatgtg
    14941 caactggtaa cgcagtatct tgtcacaaca gagtttgatg atgatacggt acaagccatc
    15001 atggacgaag cactgaaata tgagggctgg cgatacgttt acggtggagc ttccccgact
    15061 acttcttttg attgtagcgg actgacacaa tggacgtatg gaaaagctgg aattaactta
    15121 ccacgaaccg cacaacagca atatgatgtg acccagcata tcccactatc ggaagcacaa
    15181 gctggcgatt tggttttctt tcattctacc tataacgctg gctcttatat tactcatgtt
    15241 gggatatacc ttggcaataa ccgtatgttt catgcaggcg acccaatcgg ttatgccgac
    15301 ttaacaagcc cctactggca acagcattta gtgggagcag gacgaatcaa acaatgagaa
    15361 aggaagattt aatgatgaaa tttagaaaaa atcagaataa agaaaaacag ataccaaagg
    15421 aaaagaaacc tcgtgtctac tataaggtca atcctcataa aaaggttgtg attgccttgt
    15481 gggtactttt agggcttagt ttcagctttg cgatattcaa gcactttaca gctatagata
    15541 ctcatactat tcacgaaaca actatcatag aaaaggaata cgttgatact catcatgtag
    15601 aaaattttgt agagaacttt gcgaaagtct actattcatg ggagcaatcc gataagtcca
    15661 ttgataatcg aatggaaagt ctaaaaggct atctgacaga tgaacttcaa gctctcaatg
    15721 ttgatacagt acgcaaagat attcctgtat cgtcttctgt aagaggattt cagatatgga
    15781 cggtagagcc aactggcgac aatgagttta atgtaaccta cagtgtagac cagctcatta
    15841 cagagggaga aaatacaaag accgtccact ctgcttatat agtgagtgtc tatgtagatg
    15901 gttctggaaa tatggtactg gttaagaatc cgaccattac caacatacct aagaaatcaa
    15961 gttataaacc aaaagccatt gaaagtgagg ggacggttga ttccattaca accaatgaaa
    16021 tcaatgagtt tttaacgacg ttcttcaagc tctatcctac agcgacagcc agtgaacttt
    16081 cctactatgt gaatgacggg atattaaaac caatcggaaa agagtacatc tttcaagaac
    16141 tggtaaatcc tattcacaat cgtaaggata atcaagtcac ggtatcgctg acagtggagt
    16201 atatcgacca gcagaccaaa gcaacgcagg tatctcaatt tgatttggta cttgaaaaga
    16261 acgggagtaa ttggaagatt atagaataac aaatattggt acattattac agctattttg
    16321 taatcacgta ctctctttga taaaaaattg gagattcctt tacaaatatg ctcttacgtg
    16381 ctattattta agtatctatt taaaaggagt taataaatat gcggcaaggt attcttaaat
    16441 aaactgtcaa tttgatagtg ggaacaaata attggatgtc cttttttagg agggcttagt
    16501 tttttgtacc cagtttaaat acctttatca tgtgattcta aagtatccgg agaatatctg
    16561 tatgctttgt atgcctatgg ttatgcataa aaatcccagt gataagagta tttatcactg
    16621 ggatttttat gcccttttgg gcttttgaat ggaggaaaat cacatgaaaa ttattaatat
    16681 tggagtttta gctcatgttg atgcgggaaa aactacctta acagaaagct tattatataa
    16741 cagtggagcg attacagaat taggaagcgt ggacaaaggt acaacgagga cggataatac
    16801 gcttttagaa cgtcagagag gaattacaat tcagacagga ataacctctt ttcagtggga
    16861 aaatacgaag gtgaacatca tagacacgcc aggacatatg gatttcttag cagaagtata
    16921 tcgttcatta tcagttttag atggggcaat tctactgatt tctgcaaaag atggcgtaca
    16981 agcacaaact cgtatattat ttcatgcact taggaaaatg gggattccca caatcttttt
    17041 tatcaataag attgaccaaa atggaattga tttatcaacg gtttatcagg atattaaaga
    17101 gaaactttct gccgaaattg taatcaaaca gaaggtagaa ctgtatccta atatgtgtgt
    17161 gacgaacttt accgaatctg aacaatggga tacggtaata gagggaaacg atgacctttt
    17221 agagaaatat atgtccggta aatcattaga agcattggaa ctcgaacaag aggaaagcat
    17281 aagatttcag aattgttctc tgttccctct ttatcatgga agtgcaaaaa gtaatatagg
    17341 gattgataac cttatagaag ttattactaa taaattttat tcatcaacac atcgaggtcc
    17401 gtctgaactt tgcggaaatg ttttcaaaat tgaatataca aaaaaaagac aacgtcttgc
    17461 atatatacgc ctttatagtg gagtactaca tttacgagat tcggttagag tatcagaaaa
    17521 agaaaaaata aaagttacag aaatgtatac ttcaataaat ggtgaattat gtaagattga
    17581 tagagcttat tctggagaaa ttgttatttt gcaaaatgag tttttgaagt taaatagtgt
    17641 tcttggagat acaaaactat tgccacagag aaaaaagatt gaaaatccgc accctctact
    17701 acaaacaact gttgaaccga gtaaacctga acagagagaa atgttgcttg atgccctttt
    17761 ggaaatctca gatagtgatc cgcttctacg atattacgtg gattctacga cacatgaaat
    17821 tatactttct ttcttaggga aagtacaaat ggaagtgatt agtgcactgt tgcaagaaaa
    17881 gtatcatgtg gagatagaac taaaagagcc tacagtcatt tatatggaga gaccgttaaa
    17941 aaatgcagaa tataccattc acatcgaagt gccgccaaat cctttctggg cttccattgg
    18001 tttatctgta tcaccgcttc cgttgggaag tggaatgcag tatgagagct cggtttctct
    18061 tggatactta aatcaatcat ttcaaaatgc agttatggaa gggatacgct atggttgcga
    18121 acaaggatta tatggttgga atgtgacgga ttgtaaaatc tgttttaagt acggtttata
    18181 ctatagccct gttagtaccc cagcagattt tcggatgctt gctcctattg tattggaaca
    18241 agtcttaaaa aaagctggaa cagaattgtt agagccatat cttagtttta aaatttatgc
    18301 gccacaggaa tatctttcac gagcatacaa cgatgctcct aaatattgtg cgaacatcgt
    18361 agacactcaa ttgaaaaata atgaggtcat tcttagtgga gaaatccctg ctcggtgtat
    18421 tcaagaatat cgtagtgatt taactttctt tacaaatgga cgtagtgttt gtttaacaga
    18481 gttaaaaggg taccatgtta ctaccggtga acctgtttgc cagccccgtc gtccaaatag
    18541 tcggatagat aaagtacgat atatgttcaa taaaataact tagtgtattt tatgttgtta
    18601 tataaatatg gtttcttgtt aaataagatg aaatattttt taataaagat ttgaattaaa
    18661 gtgtaaagga ggagatagtt attataaact acaagtggat attgtgtcct gtatgtggaa
    18721 ataaaacacg attaaagata agggaagata ctgaattaaa aaaattcccc ctctattgtc
    18781 cgaaatgcag acaagaaaat ttaattgaaa taaagcagtt caaagtaact gtgattacag
    18841 agccagacgc aaagacgcag agccgataaa atgagattaa tacaatctca ttttatcggc
    18901 tctttccgtt atgtatggat tcttttaatt agtcttcgat gtttcttgct tcgttgatac
    18961 cgctggctaa agattccatt aaggatagtt ctttgtctgt aaagctatcc atgtatttct
    19021 ctatctgtaa tcgtcgggtg ctttttacca agttattagc aggtaagaaa aattcatcaa
    19081 cggaaacatg aagtaacgat acaaggtcat aaagaacttg tatgctgggg tgttgccctt
    19141 tattttcaat attagttaag taccgtgggt caatttcaat caatgctccc acttgttcac
    19201 gagttaaacc tcgtttcaat cgagcttctt taatggctaa accaaaggct ctaaaatcat
    19261 atttatcttc ttttttacgc atagtagacc acctctatac attttattgt tcctactgaa
    19321 ttaaaaacag gtatagaaaa acgtgttata tggtttatag gtttatattt aataaaaagc
    19381 actactaaac gccaataaaa aaaaccgtta tatggtagtg ctatttacgc tgttaaaata
    19441 ttgtatatta cttccaaatg gcggtttgtt ggaggtcaac gtcgccatga agtacatcat
    19501 atacaataaa tttccttaca ttgggttctt gtcaaaaaaa gtcgtctatc tgcaatagat
    19561 aagtacgtcc accaatgtgg ttttataaat catatagata gaataacaga agcatgtaaa
    19621 cagagaaata aatctgttta tatgcttttt tggctattca gaactttttt acaaagttta
    19681 tttatcagta atgcaacaaa tccccctttc acattgggac taagagtgaa aggagataaa
    19741 cgagcaaggc tcacttcctt tcctagacag aaagggggtg agaaacatga aaccatcttc
    19801 ttttcagacc acaatagaaa atcagtttga ctatatctgt aaacgtgcta tggaagacga
    19861 gcgaaagaat tatatgcttt atctttcaag gattgcaaag cgtgaggtgt ccttttcgga
    19921 tgttggcgat tatcttgtta gccagtttgc gacaacagat aactattcaa ctgactttca
    19981 gatttttaca ctcaatgggt tatcagtagg cgttgaaaat gatttgttga gtgaagcatt
    20041 acgtgagttg ccagacaaga aacgtgaaat tctactgctg ttttacttta tggacatgag
    20101 cgattcagaa attgcagacc tgttgaaatt gaaccgttct actgtctatc ggcatagaac
    20161 cagtggacta gccttaatta aaaagtttat ggaggaattt gaagaatgaa aacacaatat
    20221 cctatgattc cctttcctct cattgtaaag gcaacagatg gcgataccga agcgattaac
    20281 cagattctac atcattacag agggtacata acgaagcgtt ccctacgact tatgaaagat
    20341 gaatatggca atcaaagtat ggtcgttgat gaagtcttac gtggaagaat ggaaaccaga
    20401 ctgattacaa agattttgtc atttgaaatt aagtaatatc ctctctcctt tcgtggaagc
    20461 gtgctaaacc attccacgct tcccgaacag ggaggtttgt tattccacca aagcatattg
    20521 agctttcaat gtgttttgat aggctaacga gccattgttc tttgaaaact gaataaaagt
    20581 aatcgaatac gtttcgataa gaaaagagcc aacggaacta accgccatga cctatcttat
    20641 aaagatagcg agcgattcat gttagtgatc cgagaagcaa tctttagcag gattgcctgc
    20701 aacgacattc ttatcgtgat aatgatactc ccatacagtc aatagtccga gcgtgataaa
    20761 accgtcgcag gcaatgagta tggctacatg agaaccatgc aggggtggaa ctcccgtgag
    20821 ctttgctaaa gctgttcgat tgctggtaaa acaactttta tgaaatccaa ataagtgatt
    20881 tggaaaggag gattttatga agcagactga cattcctatt tgggaacgtt ataccctaac
    20941 cattgaagaa gcgtcaaaat attttcgtat tggcgaaaac aagctacgac gcttggcaga
    21001 ggaaaataaa aatgcaaatt ggctgattat gaatggcaat cgtattcaga ttaaacgaaa
    21061 acaatttgaa aaaattatag atacattgga cgcaatctag cgtcgccaaa gggtcttgta
    21121 tatgataaaa tagtattaag tcgtatcaag gctctttcca taaaggaaag gagcaaatgc
    21181 catgtcagaa aaaagacgtg acaataaagg tcgaatctta aagactggag agagccaacg
    21241 aaaagacgga agatacttat acaaatatat agattcattt ggagaaccgc aatttgttta
    21301 ctcgtggaaa cttgtggcta cagaccgagt accagcagga aagcgtgatt gtatctcact
    21361 tagagagaaa atcgcagagt tacagaaaga cattcatgat ggtattgatg ttgtaggaaa
    21421 gaaaatgaca ctctgccagc tttacgcaaa acagaacgct caaagaccaa aggttagaaa
    21481 aaacactgaa actggacgca aatatcttat ggatattttg aagaaagaca agttaggtgt
    21541 aagaagtatt gacagtatta agccatcaga cgctaaagaa tgggctatta gaatgagtga
    21601 aaatggttat gcttatcaaa ccatcaataa ctacaaacgt tctttaaagg cttcattcta
    21661 tattgctata caagatgatt gtgttcggaa gaatccattt gactttcaac tgaaagcagt
    21721 tcttgatgat gatactgtcc ctaagaccgt actaacagaa gaacaggaag aaaaactgtt
    21781 agcctttgca aaagctgata aaacctacag caaaaattat gatgaaattc tgatactctt
    21841 aaaaacaggt cttcgtattt cagagtttgg tggtttgaca cttccagatt tagattttga
    21901 gaatcgtctt gtcaatatag accatcagct attgagagat actgaaattg ggtactacat
    21961 tgaaacacca aagaccaaaa gtggcgaacg tcaagttcct atggttgaag aagcctatca
    22021 agcatttaag cgagtgttag cgaatcgaaa gaatgataag cgtgttgaga ttgatggata
    22081 tagtgatttc ctctttctta atagaaagaa ctatccaaaa gtggcaagtg attacaacgg
    22141 catgatgaaa ggtcttgtta agaaatacaa taagtataac gaggataaat tgccacacat
    22201 cactccacat agtttgcgac atacattctg taccaactat gcaaatgcag gaatgaatcc
    22261 aaaggcatta cagtacatta tgggacatgc taatatagcc atgacgctga actattacgc
    22321 acatgcaaca ttcgattctg caatggcaga aatgaaacgc ttgaataaag agaagcaaca
    22381 ggagcgtctt gttgcttagt agtacaaatg aatttactac ttatttacca cttctgacag
    22441 ctaagacatg aggaaatatg caaagaaacg tgaagtatct tcctacagta aaaatactcg
    22501 aaagcacata gaataaggct ttacgagcat ttaagaaaat ataaaaagat aattagaaat
    22561 ttatactttg tttaatttaa aaaatatcca gataaaaact ttagtaattt agattattgc
    22621 ttaataggaa gtaaatgttc aaatagtttt ataaaagaaa agttgattac tttttttaaa
    22681 gtgagaatac cagatatatt aaaagacaaa agtatattaa aagcagagtt atttattcat
    22741 attgattcaa ataagaatca tatttttaaa gaaaaagtag atattgaaat taaaagaata
    22801 agtgaatatt ataatttacg aactataaca tggaatgata gagtgtctat ggaaaatatc
    22861 agggggtatt taccaattgg tataagtgat acatccaact atatttgttt aaatattaca
    22921 ggaactataa aagcatgggc aatgaataaa tatcctaatt atgggttagc tttatcttta
    22981 aattaccctt atcagattct tgaatttaca tctagtagag gttgtaacaa accgtatata
    23041 cttgtaacat ttgaagatag aattatagat aattgttatc ctaaatgtga gtgtcctcca
    23101 attagaatta caggtccaat gggaccaaga ggagcgacag gaccaagagg agcaacaggt
    23161 ccgacaggaa gtacaggacc aacgggagaa ataggcccaa caggaaatac gggagcaaca
    23221 ggagcgacag gaagtatagg accaacggga gtaacaggtc caacaggaaa tacaggagta
    23281 acaggagaaa taggcccaac aggaaatacg ggagcgacag gaagtatagg accaacggga
    23341 gcaacaggtc caacaggaaa tacaggagcg acaggaagta taggaccaac gggagtaaca
    23401 ggcccaacag gaaatacggg agtgacagga agtatagggc caacgggagt aacaggtcca
    23461 acaggaaata caggagtaac aggagaaata gggccaacag gtccaacagg aaatacagga
    23521 gcgacaggaa gtatagggcc aacgggagta acaggcccaa caggaaatac gggagtgaca
    23581 ggaagtatag ggccgacggg agtaacaggt ccaacaggaa atacaggagt aacaggagaa
    23641 atagggccaa caggtccaac aggaaataca ggagtaacgg gagaaatagg accaatggga
    23701 gcaacaggtc cgacaggaaa tacaggagtg acaggaagta tagggccaac gggagtaaca
    23761 ggtccaacag gaaatacggg agtgacagga gaaatagggc caacaggaaa tacgggagcg
    23821 acaggaagta taggaccaat gggagcaaca ggtccgacag gagcaacagg cccaacggga
    23881 gcaacaggcc caacaggcaa ttcctcgcag ccagttgcta acttcctcgt aaatgcacca
    23941 tctccacaaa cactaaataa tggagatgct ataacaggtt ggcaaacaat aataggaaat
    24001 agctcaagta taacagtaga tgcaaatggt acgtttacag tacaagaaaa tggtgtgtat
    24061 tatatatcag tttcagtagc attgcaacca ggttcatcaa gtataaatca atattctttc
    24121 gctatcctat tcccaatttt aggaggaaaa gatttggcag gtcttactac tgaaccagga
    24181 ggtggaggaa tactttctgg atattttgct ggttttttat ttggtgggac gacttttact
    24241 ataaataatt tttcatctac aacagtaggg atacgaaatg ggcaatctgc aggaactgcg
    24301 gctactttga cgatatttag aatagctgat accgttatga cttaaaatgt gtctaaaata
    24361 atcttaaaaa ctatttagat tttatttaaa tgataaaagt atttttatat attgagtttt
    24421 acctatttta gaatgagtaa aataacaata ataataaaat atattcataa aaattttaaa
    24481 tttatggatt tttattttac tttattatca atatatgtat aataaaaaac tgtctcaaat
    24541 atagatttga gacagttttc gttatttaaa aattttatat tatttaaagt ttttgattgc
    24601 agtagttaaa ttaggaacta attgtttttt tcttgataca acacctggtg caaatgtacc
    24661 ttgaacacct tcaataccaa atgcgttagc aattaggcta tcctctgctt tatagattaa
    24721 ataagagcct tctttaatta tatccgtaac tgcaagaact aatttatcat aatcagttga
    24781 atttatataa gataaaaact catctttttt agcaaatata gagtctatgt ctaaagtaaa
    24841 tacttgtcca ataccaactc tatgtccacc catattaaat tctttaaaat ccatatttac
    24901 tatttcttct atagtatatt catctaaaga agtaccacat ttaaacatat ccatagcgta
    24961 tttttccatg tctacttttg ctattttact taattcttca caagctttct tatccatatc
    25021 agttgttgtt ggagacttaa ataataatgt atctgataat atagcagata aaagaagccc
    25081 agctatttca taaggtatct caacattatt ttctttgtac atttgataaa ttatagtact
    25141 attgcatcca acaggcataa ctctaaatga cataggaaca tcagtagaaa taccaccaag
    25201 tttatgatgg tcaattattt caactatgtt tgcttgttca attccatcag cactttgagc
    25261 atattcgtta tggtcaacta aaacaacatt ctttttagat gggtttaata gatgaccttt
    25321 tgaaactaaa cctaaaaact tattatcatc atctaatatt ggataagatc tgaagtttgt
    25381 ttctagcata atctctttta tttcatccat ataatcatct tctttgaatg taattaaatt
    25441 ttctgtagct tctatagagt ctaagaaact acattgattt attacattag aagttttata
    25501 attgtcactt tcaacagaaa caacagacac tccatttatc ttagcaagag ctagcaaatc
    25561 ttttgaaatc tctgtatgat tagtaagtat taaaagttta acttttgtat caatagcata
    25621 ttcaatacta tcatatctat ctccaacaat agctatgtct ccttctttta aagtctctat
    25681 tagagtctcc attccaaatg ataaagtgac tactctacct tttacatctt caccacagtc
    25741 cacaagaata gtaccattaa ggtttttaca aatgtttgat agtgatgtgt gtatagttgt
    25801 aaagtgttga tgaagtaggt ttttagctat ttctttcata gtaatgatac cagcaaaagt
    25861 tccatcatca taagttacag gtagtgtttt aacatttttt tcatccataa ggttataagc
    25921 ctctaatata gatgaagttg gtgttaaagg tactacttta tcgtaattta aatctctaac
    25981 ttgtattcta acattatcta aaatttcagg tgcatctacc ttaaagtaat ccaaggcata
    26041 tttagcttct tttctgatat ctcctaaagc ataagctgtt gcttttattc ctagtttgtt
    26101 ctttaaatgt gctaacgaaa ttgcagaaca tattgaatct gtatctggat ttttatgtcc
    26161 gaaaactaat aaactcattt taatactcct ttcaaaactt gaatataata ataattgata
    26221 aaaatattat atcaaaaggt agtgagtatt tgttgtaaaa agaaaaaaa
//
LOCUS       DAEPXF010000040        26129 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain HB27
            SAMN13376389-rid12479423.denovo.042, whole genome shotgun sequence.
ACCESSION   DAEPXF010000040 DAEPXF010000000
VERSION     DAEPXF010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13376389
            Sequence Read Archive: SRR10518432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 26129)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26129)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001069624.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001069624.1
            Genome Coverage       :: 315x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/15/2021 01:54:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,165
            CDSs (total)                      :: 4,076
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 89
            rRNAs                             :: 10, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 10 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 19 of 69
            Pseudo Genes (incomplete)         :: 44 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 10 of 69
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26129
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13376389-rid12479423.denovo.042"
                     /strain="HB27"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="China: Hebei"
                     /collection_date="2017"
     repeat_region   532..826
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=532..560
                     /rpt_unit_seq="gttttagattaactatatggaatgtaaat"
     gene            1121..1696
                     /locus_tag="KRL67_001450"
     CDS             1121..1696
                     /locus_tag="KRL67_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002589187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SHOCT domain-containing protein"
                     /protein_id="HBH1522342.1"
                     /translation="MNAVELCRDNFGNFVKNTLIQIVYLGGHPLFNREEMICLLIRSD
                     KIVVKAFGTGPVPIVDVFEVSYSDIKSVSIEKEEEVIRRYTATRIALFGPFALAMQKA
                     ELNKKEYLIIECNDFILSFDKNDEALATVYKNLIEYRKNNGIGNVSESEVEKNKITNP
                     LEQIKTLKELLDMDAITQEEFDVKKKELLNL"
     gene            2000..2173
                     /locus_tag="KRL67_001451"
     CDS             2000..2173
                     /locus_tag="KRL67_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HBH1522343.1"
                     /translation="MSSKMGRPKVDNPKNIDVKVRFDENTHKKLLDYCEKENLTRTEA
                     IRKGVDLLLEEDR"
     gene            2297..3124
                     /locus_tag="KRL67_001452"
     CDS             2297..3124
                     /locus_tag="KRL67_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin Bro"
                     /protein_id="HBH1522344.1"
                     /translation="MNNELMNFENNELGIKIRTIKYEDGSIGINAEDTAVGFGWCKIE
                     NKNGKEYKSVRWERMNEFCIEFGFDHKWAKDDYIPESLFYMLGMKAKNEIAVKFQTWL
                     AVDVLPSIRQTGAYITNNANPKKLREKASEIEKLQLAYNSTSMLKELLDGAGFDNKSK
                     LLTAKTLYKKAGIDLPIEIEEEESFFDTKQIASKLKIYSKSNKPAQLAVCEIIKKIKL
                     EENEVKGVWETNGSWTGTVNKYTKSVIDKVRTWIEENNRPAKIEGEKKNFHVVYKIE"
     gene            3173..3349
                     /locus_tag="KRL67_001453"
     CDS             3173..3349
                     /locus_tag="KRL67_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH1522345.1"
                     /translation="MYENLLKEYNLKTDEDVEYFVKFATLLHKLKQDNEEKFQEYAEI
                     LRGILREQEERKNK"
     gene            3780..4340
                     /locus_tag="KRL67_001454"
     CDS             3780..4340
                     /locus_tag="KRL67_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015984850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein L7/L12"
                     /protein_id="HBH1522346.1"
                     /translation="MFCSNCGAEITGVGKFCSSCGVAVETEIIEDNNIKSNDLIVDAN
                     GIEINMTEIYRKYRKEKVNAIKNVMEISGLNIKEAKKIVDSSFEELKINFIDDTMSNS
                     EKEKIINTQNRKNNIEKAQQESVACCPKCGSTSLSAQKKGFGIGKAVAGATIAGGIGL
                     VAGNLGAKKVRVTCLNCGKQFWAGKK"
     gene            4416..6782
                     /locus_tag="KRL67_001455"
     CDS             4416..6782
                     /locus_tag="KRL67_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tape measure protein"
                     /protein_id="HBH1522347.1"
                     /translation="MATIQTSIRIFDGMTPAFRHMTNAMNIVLSSFEQLQRTSSNAID
                     ANSIRTAREELARAEAGFDRLEQQIREADEQQKRFNDDIRKGASSTDKLVENAKRLVA
                     TYIGLRSVGGLINLSDQMTSTNARLAMINDGQQSDGGLNKMIFQSAERARASYLDTAK
                     IVSRVGMNAGKAFSSTKEIVAFAEQLNKKFVIAGATTEETNSALLQLTQGLGSGVLRG
                     EELNAVFESAPNIIQSIADYLEVDIGKIRGMASEGMLTADIVKNSLLSAAEQTNAEFE
                     KMPYTFGQIFTSIKNNAVMIFGVIQKKIEQSMSSKGFRTFIDNFIDSLYVLGAVAFSI
                     FNGIINILGSPFFQAFVNAIIVGVSLIVQVLGWVITQALNITNVFAQNWSIIAPIVLG
                     VAAAMLVYNNALLLSIANKVKDIALSAKTLAMNFAHIIAESYRAAALVASTIAQDGLN
                     AAMAACPITWILYGIIAIVVAFFVAIAVINKFAGTSYSAIGIVAGALSGLTAFIINSV
                     FFWINVFISFAEFFTNVLDHPVYSVKKLFFNLATAVLNNLISMTKGCDEFATNLANSI
                     IDGINGALKAWNKFVDVLNKFGGLADKLGLGKADMVGHTKSITSTLQKAKGDLNKWLG
                     AEPEGYKSFKQLEYKNVGDWAKNGYAFGQNLENKLKDAFDINKIAEKAKKDLGLDDLW
                     DKKYGLGDGLGSAGLNSPLSDAAKGAKDTAGNTAKMAKTMDKSQEDLKYLRDIAEQEV
                     INKYTGVNIKIDMNNTNNISKDTDLDGIVNVLTEKLNDAMVVSAEGVV"
     gene            6798..7499
                     /locus_tag="KRL67_001456"
     CDS             6798..7499
                     /locus_tag="KRL67_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HBH1522348.1"
                     /translation="MAYDFYLDGVQLPIPPPKLEIKVTNKNKTVDLINTGEVNILKKE
                     GLSEISFEAEFTHNKLPFYRGTFRDVQFFLSKLELLKTDCKPFQFIVSRELGNKVLFN
                     TNMKVSLEEYNIVEDAENASDTKVAIKLKQYRDYSTKKLVLATPEKTNYGRTPPPVMK
                     PKEFRPDSSNKPNAKTYTVKAGDSLWSICQKQLGNGSLYKKVYELNKSMMDKANKGKK
                     VPKYTIYKGQVLKLG"
     gene            7492..9456
                     /locus_tag="KRL67_001457"
     CDS             7492..9456
                     /locus_tag="KRL67_001457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HBH1522349.1"
                     /translation="MDDELVLANDRDVRLVIAHWEDFYEPVVLDGITWEIERRGTPSK
                     LEFTIVMDDILEFCEGNSVRLYYKGVGIFYGYIFQKKRDKENHIKIVAYDQLRYFKNK
                     DTYVYSNKTASELVKMLAKDFNLKYNVIEDTKYKLSRVEENKTLFDMILTALDDTLRE
                     KKEMYTLYDDFGRITLKNVASMKLDTVMNNDVIEDFDYNSSIDSDTYTKIKLVRDNEE
                     SGKRDVYIAQDSTHMRSWGILQMFETVDKNMNEAEIKQKCDILLKLYNKKTKSLSLKN
                     ALGDIRVRAGCLVPVFLNLGDIKLQNYMLVEKVKHTFENNSHFMDLTLVDGDEFASYS
                     SSSYSSGNTNNKNEKQNGPAQSTTSKEDNDMINKLNKVFKNKLSNTGNIFVKYSNAYK
                     VNAALMAAISIHETGNGSSSLCKNKNNFFGMKGMSFSSVDEGIKKGISNLSRNYIHTG
                     RKTLERIRDKYAPLYDSPLNKDWVPGVGKFYKQITGSTYTSNNAGTGVGSNEEAEKNL
                     KDTTYQVQNNNSNTLTNNNSKVSKVIQEAKNQLGKPYAWGGNGPKSFDCSGLMVWAFK
                     RGAGINLPRVSADQSKDSRGKLLCNINDVKAGDLVFFKNEQGKVHHVGLYIGNDQYIH
                     APQTGDVVKISSLSGRQKKKHDFERARRFF"
     gene            9470..9730
                     /locus_tag="KRL67_001458"
     CDS             9470..9730
                     /locus_tag="KRL67_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2577 domain-containing protein"
                     /protein_id="HBH1522350.1"
                     /translation="MSQELLQIIKKAAMDAVETSNPIQIAFGTIESVNPLVVKIEQKA
                     SFEESFLIQTDTFKKYTDKKIGDKLVLIRMQGGQQYLILDRM"
     gene            9735..10154
                     /locus_tag="KRL67_001459"
     CDS             9735..10154
                     /locus_tag="KRL67_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2634 domain-containing protein"
                     /protein_id="HBH1522351.1"
                     /translation="MLPSDNLDYDIEDVSIINFDVRQEPSKTFKLNIEKNRVDGICDD
                     VEALKQTIFLILNTERYEHLIYSRNYGVELNDLIGEPISYVIPELERRITEALIQDDR
                     IENIDNFEFQNIKGKVQCRFSVHTKYGNIKAEKVVSV"
     gene            10155..11201
                     /locus_tag="KRL67_001460"
     CDS             10155..11201
                     /locus_tag="KRL67_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HBH1522352.1"
                     /translation="MFELMTFENIIKRMLDSVPDTFDKREGSIIYNALAPVAIELTET
                     YIAMDELLDQTFVDTASYYYLEKRCKERGITPLSATNTIAKGVFNIDIPLDSRFNLGE
                     YNYIAIERISEKTYKMKCETAGPIFELGKLIPIEYIDGLETTELTEILINGEDEESED
                     SLRQRYYDSLNSQSFGGNIQNYKDEVNKLPDVGGVKVYPVWNGGGTVKLVIINSNFKV
                     PSTDLVNLVQEEIDPNMKGEGLGLAPIGHRVTVEGVTSTTINISAEITYKSGYTWENI
                     KSIAEEAIDDYLNELNMSWEDEENLIVRISQIETRLLSIDGVLDITNTMINEVKSNLT
                     INSNSIVVRGEVVG"
     gene            11194..11811
                     /locus_tag="KRL67_001461"
     CDS             11194..11811
                     /locus_tag="KRL67_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmfQ family protein"
                     /protein_id="HBH1522353.1"
                     /translation="MDKEINLINYLPQILQDKEEYIKVFNVENKEIKTLHDKLKDLSN
                     DQFLEDLTISGIKRWEKIMSITPKSNESLEDRRFRIFSKYISKLPYSERFLRNWLDNV
                     VGEGNYELTINNATYNIHLESDARNQDWFEEVHSFVSNIKPCNMTLDYTRVLISKDNY
                     MNFGITTLMGQEITIYPWSPPDIETYGEIDVLTGNGVGYQEITIF"
     gene            11823..12815
                     /locus_tag="KRL67_001462"
     CDS             11823..12815
                     /locus_tag="KRL67_001462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015984856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBH1522354.1"
                     /translation="MAIDKSYYTIITDVGKAKIANASVTGNKVGFVKIQLGDGGGSEY
                     TPTESQTALKNVVWEGNIGNTTTDETAPNCIILESLIPSSVGGFMIREIGYLDDENNL
                     IAISKYKECYKPSIEQGAVVDMKVKTVLIVSNVNNIELKIDPTIIFATLKDIQDLETK
                     IGTVNTKIDTTKTELTSNIETAKTELNTKIDQLIAGGSNVAYTQRVAIDDWVEDAESG
                     FKATVTHSLLTHRIVVNIIDATTKENIVPNFKIVDDNSIEIRSEVKVELNVYVINGNA
                     ETHFINATVDDNRVSEMTTYSSKKIEDRLVNIEEKVNGGLSNIATSVNELITYC"
     gene            12832..14142
                     /locus_tag="KRL67_001463"
                     /pseudo
     CDS             12832..14142
                     /locus_tag="KRL67_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015994364.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            14565..14912
                     /locus_tag="KRL67_001464"
     CDS             14565..14912
                     /locus_tag="KRL67_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH1522355.1"
                     /translation="MNRNNRIIYDQTGNIWLQTGEATGDIQEWSKITELNFLDVEFGS
                     IDYSKQYIESINPVTKEPIIKDIEVILTDEQKRLQALEKELSMLKEENKNRDSEIVNT
                     AFEVENIKLNNNL"
     gene            14924..15094
                     /locus_tag="KRL67_001465"
     CDS             14924..15094
                     /locus_tag="KRL67_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH1522356.1"
                     /translation="MYNLLKLMIEQKNYSTKEDLQHKIDVFYAVNRITEEQYLELTSL
                     LNKEETPAEPIK"
     gene            15125..15691
                     /locus_tag="KRL67_001466"
     CDS             15125..15691
                     /locus_tag="KRL67_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH1522357.1"
                     /translation="MTFKELVNKVRNLVLEAKNVTIEDTESKFTSENVEGALKECIDR
                     ADEAFQEADSGKTLLSTAIGSPATSEQTFQDYANYITGFKSNISNLETQLKSKYSVRY
                     GPIDGYDGNPFSANFGKSASYLIVYVYFRRSVYYYNPSGSSLGSNTGGSERAWITIDS
                     NKTGFSVHSYDTSYEPYPFTGYYIACFA"
     gene            15722..16012
                     /locus_tag="KRL67_001467"
     CDS             15722..16012
                     /locus_tag="KRL67_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH1522358.1"
                     /translation="MEEISINLLCAVAGVVISYLAFRNSSNRKIQDDTETTTKLEQQI
                     TFLCENVRDIKHDVAKFNTSFLDISERVAKVEASTKQAHLRIDEIINRIGGK"
     gene            16016..16273
                     /locus_tag="KRL67_001468"
     CDS             16016..16273
                     /locus_tag="KRL67_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HBH1522359.1"
                     /translation="MDNLISFIPEQLLILVAALYVLGVGFKKYKQLDNKYIPVVLLIL
                     GIGFSIWMLGLNPVAVLQGVICWGVAIGINQTYKQLKDGEK"
     gene            16534..17396
                     /locus_tag="KRL67_001469"
                     /pseudo
     CDS             16534..17396
                     /locus_tag="KRL67_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429958.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Abi family protein"
     gene            17475..18287
                     /locus_tag="KRL67_001470"
     CDS             17475..18287
                     /locus_tag="KRL67_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HBH1522360.1"
                     /translation="MKICITVGHSILKSGACTSADGVVNEYQYNKFLAPVLADTFRKE
                     GHKADVIICPEKQFKTKAEEKIYKIPRVNSGGYDLLIELHLNASDGQGKGSEVLYYSN
                     KGLEYATRICNKLGTVFRNRRAKLDKRLYILNSSKPTAVLIESFFCDNKEDYDKAKKL
                     GHEGIAKLIVEGVLNKNINNEGVKQMYKHTIVYDGEVDKIPATVVGWGYNDGKILICD
                     IKDYVPGQTQNLYVIGGGACEKISSITKEHYTMIKGNDRFDTLCKALDFINR"
     gene            18444..20735
                     /locus_tag="KRL67_001471"
     CDS             18444..20735
                     /locus_tag="KRL67_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding repeat-containing protein"
                     /protein_id="HBH1522361.1"
                     /translation="MKNLSKKAVILSLSLVVVSPLVHKINAQDIEKNREVRYETRKAN
                     NLENMTMEEAFPDENFRKVICDKLSILDDGNPIGISQKAIIESTKGLTLGSKSIKNLS
                     GINYFIGLENFSCRGNNLTSLDLSSNINLKELYCNENQLTSLDLSNNRELTTLHCGEN
                     NLADLVIENSKLDELNCRKNNLKSLDITKATNLTTLLCFENELTSLNLSKNQKLKILK
                     CDYNNLNDLDISTNLELQDLNCSFNQLENINLDNNKELVKLICHNNKLQNLNLKNHEK
                     LVKLYCNQEQLSTLNVENCINLEDLTCSRSNLKNLDISSNKNLKYLECVANKLTNLNI
                     ENNIELLELYCYDNEIKALDISKNIDLENLTCYKNKLDSLNTSKNKNLKYLYCSQNEL
                     TSLDVTKNTELVRLYCGENRLTSLDVSKNTKLKELDWSNQKKSTSTGGSSGSGGGSLS
                     TNEESSEPTSTPSKEKLTGADRNETSVKISQKGWNKADNIVLINDSSISDALSATPFA
                     KSKDAPILLTKNNNLNKLTEKEINRLEAKNVYIVGGLKSVDEKVVSDLKKKGLNVIRI
                     SGNDRYETSIKLAKELDKNSNLSKVVVVNGEKGLADAVSMGAISAKEEMPILLTNQND
                     DMKDIKDLIANKNISKSYVIGGESLFNNKEVNNTLPSVTKIAGSDRTETNSKVISHFY
                     SKDTLNDLYVAKNGMNKQDDLVDALSVGVLAGKTESPVVLVGNGLDNSQKEFIKNKKF
                     KNITQIGGNGNEKAFNEVENLVK"
     gene            complement(20777..20962)
                     /locus_tag="KRL67_001472"
     CDS             complement(20777..20962)
                     /locus_tag="KRL67_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HBH1522362.1"
                     /translation="MILLEQRKLRIGFNKSGKGSFTPRLILPMSWIKEMNISPDERDV
                     LVTFEDGKIIIEKTENE"
     gene            complement(21480..21656)
                     /locus_tag="KRL67_001473"
     CDS             complement(21480..21656)
                     /locus_tag="KRL67_001473"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH1522363.1"
                     /translation="MEQKESKIVYSKGGSGTYSAKISLPLSQLEKMGFTREKRKAVVI
                     FKEDEIIIKKSDEE"
     gene            22373..23050
                     /locus_tag="KRL67_001474"
     CDS             22373..23050
                     /locus_tag="KRL67_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese catalase family protein"
                     /protein_id="HBH1522364.1"
                     /translation="MFKHDKALLKEVKVERPNPQYAVLMQEQLGGANGELKAAMQYLS
                     QSFRIKDPQIKDLFLDIAAEELSHMEMVAQTINLLNGHDVDYNAVNTGEIETHVLTGL
                     SPVLINSSGAPWTANYVTVTGDLVADLLSNIASEQRAKVVYEYLYRQIDDKYVKETID
                     FLLNREEAHNALFRDALNKVKDTGSNKDFGVTEDSKLYFDLSTPGPNHDTKIDINPPS
                     FEKPLKK"
     gene            23224..25260
                     /locus_tag="KRL67_001475"
     CDS             23224..25260
                     /locus_tag="KRL67_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HBH1522365.1"
                     /translation="MDSNRKPNNLINEKSPYLLQHAYNPINWYSWNDEAFKKAKEEDK
                     PIFLSVGYSTCHWCHVMEKESFEDEEVAEIMNRNFVAIKVDKEERPDVDSVYMTVCQA
                     MTGSGGWPMTIIMTPDKKPFFAGTYFPKYSRYNRPGVIDLLENVSEKWNTSRDILIKS
                     GDEIIKALKDDFDVKNTEGDLSKEMLSSSVRVFKAIYDEKYGGFGNAPKFPSPQNLMF
                     LMKYYSIEKDKDVLKMVEKTLDGMYRGGLFDHIGFGFSRYSTDKKWLAPHFEKMLYDN
                     AMLTIAFLDAYKITKKELYKEIAIKTIDYVVREMKDKDGGFYSAQDADSEGEEGKFYI
                     FNPLEIIEVLGEEDGTFFNNYFDITSSGNFEGKSIPNLIKNKEYERHNEKIADLSEKV
                     FEYRKERTSLHKDDKILTSWNALMIVALTKAYSTLENDIYLEYSNKCLDFINNNLVNE
                     SGRLLARYRDGSSDYLAYLDDYAFLIWAYIELYESTFNMKYLEKALNLNENCINLFWD
                     YEKSGFYIYGKDSENLIARPKDLYDGAIPSGNSVQLYNLIRLAKITGDSRLEEMSYKQ
                     LKLYVDNVKSSPTGYSFYMLSLMFELYSTKEIICIFKEDSDLIAFKELISENFIPNAT
                     FLAKKYNEENTIISFLNNYRLKDDKTSYYVCQSNSCSQPINDLQKLKDMILGVE"
     gene            complement(25453..26019)
                     /locus_tag="KRL67_001476"
     CDS             complement(25453..26019)
                     /locus_tag="KRL67_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HBH1522366.1"
                     /translation="MDYSNELKELFLMNQTYATLFTLTNKIQIEGDKYFGILTSRQYM
                     TILSILHLPEEETTLNNIARKMGTSKQNINRLVANLEKNGYVDVVPSPHDKRAINVRV
                     TDLGKKVMVTCNRTGINFMADVFHEFTKDELETLWNLLKKMYRFNGEEQDGFEEDANF
                     MEYEEIDKIKSEALEEFAKRRNRVNKND"
BASE COUNT        10441 a         2918 c         4618 g         8152 t
ORIGIN      
        1 ttatataaaa aaaatggaaa aatatgtaat aattatatgt tataataatt gtagcaagga
       61 taataatcga aagtgcgaag ggtgattatt ttcatattaa acgccaaatt ccaaataagg
      121 aaggaggtga aattatatga tagttttttt attaagcata ctagctggtg ttatatcagc
      181 ttatatttat gacaaaataa aaaatcaccc agacgccaat aagggtgatt taaaaaaata
      241 atattttcac ttaacaactg aaaataatca ctctttgtag gagtaaatta tttccttgct
      301 tttattatac cacaaattgg tacagatatt caaaaataat atttttatga tataataaaa
      361 atgtagagat tttgcagtga gcgatatttg ttacaaaata tggcttaaca cttgaaatct
      421 aagatgttga gggtgtgtga taaatgttat caattgcact actcatggtt cactgcaaat
      481 ttgagagaga tgcgtatgtg taggtattgg aaatgccaag tttattttgg ggttttagat
      541 taactatatg gaatgtaaat ttatctgcgt cttctgaaat agcatactct tcaagaggtt
      601 ttagattaac tatatggaat gtaaatgttg taagaagtat cattctattt tttaatcttt
      661 ctgttttaga ttaactatat ggaatgtaaa tcttatttat agttgggaaa atggttataa
      721 atgtggtacg ttttagatta actatatgga atgtaaagca acaagaacaa cagttgattt
      781 taccttttct agccaacgtt ttatattaac tatgtggaga aaaactaaat aaacaaagaa
      841 agcacttaca aatatgtagg tgcttttgtt ttaccttata ttggaaattt gtataaagat
      901 aaattataat gattatatag atatttatga gggggattac gctatgggat tattcaaaaa
      961 gaaggaaaaa gaaaaaggca tttgtataat ttgtggtaat gaaggaaatg ctttaaaaac
     1021 tgtagataat gagtttttat gtgatgaatg ttttgaaaaa tgcagaggtg aaatagctgt
     1081 tcttggtaaa ggactaaaaa aactgacaag taaggaagtt atgaatgctg tagagttatg
     1141 tagagataat tttggaaatt ttgttaaaaa cacattaata caaattgtat atttaggagg
     1201 acatccttta tttaatagag aagaaatgat ttgtctttta attagaagtg acaagatagt
     1261 tgttaaagct tttggaacag gacctgtacc tatagtagat gtatttgaag tttcatattc
     1321 agatataaaa agtgtttcta ttgaaaaaga agaagaggtt attagaagat atacagcaac
     1381 tagaatagct ttatttggtc catttgctct tgcaatgcaa aaagcagagc ttaataaaaa
     1441 agaatatttg ataattgaat gtaatgattt tatattgtct tttgataaaa atgatgaagc
     1501 tttagcaact gtatataaga atttaattga atatagaaaa aataatggaa ttggaaatgt
     1561 tagtgaaagt gaagttgaaa aaaataagat aactaatcca ttagaacaaa taaaaacatt
     1621 aaaagaacta ttggatatgg atgctatcac gcaagaagaa tttgatgtta agaaaaaaga
     1681 attattaaat ttataatata gtaaaaatat atattttata ttcacttagt acgaatttag
     1741 tactgaacac tactttatat taattaaact acatataaat aaattcaaag ccatgaaatt
     1801 cgtggattag aaattttatt ctatactaac tatcaaaatt aaataaacat agtaagcact
     1861 tacaaatatg taggtgcttt tgttttgctc aaattggtcg gtcgagtaaa atagttagaa
     1921 aaaattggga taagttattg actttttgga acacaaataa tataattata ttatggaaca
     1981 caaaaagtga ggtgaaacaa tgagttctaa aatgggaaga ccaaaagttg ataatcctaa
     2041 aaatattgat gtcaaagtaa gatttgatga aaatactcat aaaaaattat tagattattg
     2101 tgagaaagaa aatcttacta gaacagaagc aataagaaaa ggagtagact tacttttgga
     2161 ggaagataga taaaaaagta gcccaacgcc gaccaaagca tactggacta cttaacctag
     2221 agttatctct atatgaaata ttctatcatg taaagataac tctttcaaga taataaatcg
     2281 aaagggtgat ttttgtatga ataatgaact gatgaatttt gaaaataatg aattaggaat
     2341 aaaaataaga actattaaat atgaagatgg aagtatagga attaatgcag aggatacagc
     2401 tgtagggttt ggatggtgta aaattgaaaa taaaaatgga aaagagtaca aatcagtaag
     2461 atgggaaaga atgaatgaat tttgtataga atttggtttc gaccacaagt gggcgaaaga
     2521 tgattatata ccagaatcac tattttacat gttaggaatg aaagctaaaa atgaaatagc
     2581 agtaaaattt caaacatggt tagcagtaga tgtactacca tcaataagac aaactggtgc
     2641 atacataact aacaatgcta atcccaaaaa actaagagaa aaagcaagtg agattgaaaa
     2701 gttacagtta gcttacaata gtacatctat gttaaaagaa ctattagatg gtgcaggctt
     2761 tgataataaa tcaaaactat taacagcaaa gacattatat aaaaaggcag gcattgattt
     2821 acctatagaa attgaagaag aggaatcttt ctttgacaca aaacaaatag catctaaact
     2881 gaaaatatat tctaagagta ataaaccagc acagttggct gtttgtgaga ttattaaaaa
     2941 gataaaatta gaagaaaatg aagtaaaagg agtttgggaa acgaatggtt cttggactgg
     3001 tactgtaaat aaatatacaa agagtgtaat agataaggtt agaacttgga tagaggagaa
     3061 taatagacca gctaagattg aaggtgaaaa gaagaatttt catgtagtgt ataagattga
     3121 gtaaatttga ttgtattaaa tagtttagtt tatttttggg gggattaata caatgtatga
     3181 gaatttactt aaggagtaca atttaaaaac tgatgaagat gtagaatact ttgtaaagtt
     3241 tgctacatta ttacataaat taaaacagga caatgaagaa aaatttcaag agtatgcaga
     3301 gatattgaga ggtattctta gagaacaaga agagagaaaa aataagtaaa taaatagata
     3361 aagcacttgg atattatgat gtttcaggtg ctttgtttgt aaaaaaatgg tataatggaa
     3421 atagagaatt atattaacaa tgtggtatgt aaataatgaa ttttgagaat ctacatagga
     3481 ttgaactact aattttatat taactatgtg gtatgtaaat actaaattag aggtcgagaa
     3541 ggctattgaa tcaaaaggtt ttatattaac tatgtggtat gtaaattaca cattttctca
     3601 aatttggact tcaattaaaa atgttttata ttaactatgt ggacttaaaa ttaaaaataa
     3661 tcaaaaacac ttacttgaat agtaggtgtt tttttattga aaggatgtga ttataatgta
     3721 aaaattttac ttatatagta taataatctt ataaaattat tgtgctaggg ggatttgtta
     3781 tgttttgttc gaattgtggt gcagaaatca caggcgtagg caagttttgt tcaagttgtg
     3841 gggttgctgt agaaactgaa attattgaag ataataatat taaatcaaat gacctaatcg
     3901 ttgatgcaaa tggaatagaa ataaatatga ctgaaattta tagaaaatat agaaaagaaa
     3961 aagtaaatgc aataaaaaat gtaatggaaa taagtggttt gaatataaag gaagcaaaaa
     4021 aaatagtgga ttcttccttt gaagagttaa aaatcaattt tattgatgat actatgagca
     4081 attcagaaaa ggaaaaaata ataaatactc aaaatagaaa aaataatatt gaaaaagctc
     4141 aacaagaatc agttgcttgt tgtcctaaat gtggttcaac atctctatca gctcagaaaa
     4201 aagggtttgg tataggaaaa gctgtagcag gggcgactat agcaggaggt attggtctag
     4261 tagcaggcaa cttaggagca aagaaagtta gagtaacatg tttgaactgt ggcaaacaat
     4321 tttgggcagg caaaaaatag atgtattcat aataaaaaca cttactaatt tagtaagtgt
     4381 ttttattatc taaattaaca gaaaggaggt taaaaatggc aactatacaa acatctatcc
     4441 gaattttcga cggaatgaca cctgctttta gacacatgac taatgctatg aatattgtat
     4501 taagttcatt cgagcaatta caaagaacat ctagcaatgc tatagatgct aatagtatta
     4561 gaacagctag agaagaacta gcacgtgcag aagctgggtt tgatagatta gaacaacaaa
     4621 taagagaagc tgatgaacaa caaaaaagat ttaatgatga tattagaaaa ggagcaagta
     4681 gtacagataa gttagtagaa aatgctaaaa gattagtagc aacttatatt ggattgagaa
     4741 gtgttggtgg tttgattaat ttaagtgacc aaatgacgag tactaatgcg agactagcta
     4801 tgataaatga cgggcaacaa tctgatggag gactcaacaa aatgatattt caatcagcgg
     4861 aaagagcaag agcatcttat ttagatactg caaaaattgt aagcagagtg ggcatgaatg
     4921 caggcaaagc atttagtagt acaaaagaaa tagtagcatt cgcggaacaa cttaacaaga
     4981 aatttgtaat tgcaggagca acaacagaag aaactaattc agcactttta caacttacac
     5041 aaggtttagg aagtggcgtg ttaagaggcg aggaactaaa tgctgtgttt gagtcagcac
     5101 ctaatattat tcagtctatt gccgattatc tcgaggtcga tatagggaag ataagaggta
     5161 tggcaagtga aggaatgtta acagcagata ttgtaaaaaa ctccttactt tcagcggcgg
     5221 agcagactaa tgcagagttc gagaagatgc cttatacatt tggacagatt ttcacatcta
     5281 tcaagaataa tgcagtcatg atatttggtg ttatacagaa aaaaatagaa caatctatgt
     5341 ctagtaaggg ttttcgaacc tttatagata attttataga ttcgttatat gttttaggag
     5401 cagttgcatt tagtattttt aatggaatta taaatatatt aggaagtcca ttttttcaag
     5461 catttgtaaa tgctattatt gtaggtgtta gtctgatagt gcaagtgctt ggctgggtaa
     5521 taacacaagc attaaatatt actaatgtat ttgctcagaa ctggagtatt attgcaccaa
     5581 tagtacttgg agttgcagct gctatgttag tatataacaa tgcactatta cttagtatag
     5641 ctaataaagt aaaagatatt gcgttatctg ctaaaacttt agcgatgaat tttgctcata
     5701 taatagcaga gtcttataga gcagcagcat tagtagcaag cacaattgca caggacggat
     5761 tgaacgcagc aatggcagct tgccctatca cttggatttt atatggaatt atagccatag
     5821 ttgttgcctt ttttgtagct atagctgtaa taaataaatt cgcaggaaca tcttattcag
     5881 caattggaat tgttgcagga gcattatcgg gtttaacagc atttattata aatagtgtat
     5941 ttttctggat taatgttttt atatcatttg ctgagttttt tacaaatgta ttagaccatc
     6001 cagtttattc agtaaagaaa ttatttttta atttagccac agcagtatta aataatctta
     6061 tatctatgac aaaaggatgt gatgaatttg ctactaattt agcaaactct attatagatg
     6121 gaattaatgg ggcattaaaa gcttggaata aatttgtaga tgtcttaaat aaatttggag
     6181 gacttgcaga taagttagga cttggaaaag ctgatatggt tggtcataca aagtctatta
     6241 caagtacact acaaaaagct aaaggtgatt taaataagtg gcttggagca gagccagagg
     6301 gatacaaatc ctttaaacaa ctagagtaca aaaatgtcgg agattgggct aaaaatggat
     6361 atgcatttgg tcaaaactta gaaaacaaat taaaagatgc ttttgacatc aataaaatag
     6421 cagaaaaggc taaaaaggat ttaggactag atgacctttg ggacaaaaaa tacggtcttg
     6481 gtgacggact cggttcggca gggcttaatt ctccgctcag cgacgctgca aaaggagcaa
     6541 aagatacagc aggaaacaca gctaaaatgg caaagacaat ggataaaagt caagaggact
     6601 taaaatactt aagagatata gctgaacaag aagtaataaa caagtataca ggagtcaaca
     6661 ttaaaattga catgaacaat acaaacaaca taagtaaaga tactgactta gatggaatag
     6721 ttaatgtatt aactgaaaaa ttaaacgacg ccatggttgt atcagcagaa ggcgtagttt
     6781 agaaaggagg tgtaatcgtg gcttatgact tttatttaga tggagtacaa ttaccaatcc
     6841 ctccacccaa gttagaaatt aaagtgacaa acaaaaataa aacagttgat ttgataaaca
     6901 ctggagaagt aaacatacta aaaaaagaag gattatctga aataagtttt gaagcagagt
     6961 ttacacataa taaactacca ttttatcgtg gaacttttag ggatgttcaa ttctttttaa
     7021 gtaaactaga attactaaag acagattgta agccatttca atttattgta tctcgtgagt
     7081 taggtaacaa ggtcttattt aacactaata tgaaagtgtc attagaagaa tataacatag
     7141 tagaagatgc agaaaatgcc tcagatacaa aagttgcaat aaagttaaaa caatatagag
     7201 attactcaac taaaaagtta gtacttgcaa cacctgaaaa gacaaattat ggtaggactc
     7261 cccctccagt catgaaacca aaagaattta gaccagattc atccaataag ccaaatgcta
     7321 agacatatac agtcaaggca ggagattctc tttggtcaat ctgccagaag caacttggta
     7381 atggttcatt atataagaag gtatatgagt taaataaatc aatgatggat aaagctaaca
     7441 agggtaaaaa agtacctaaa tatactattt acaaagggca ggtgttaaaa cttggatgat
     7501 gaattagtat tagcaaatga tagagatgta agattagtaa tagctcattg ggaagatttc
     7561 tacgaacctg tagttttgga tggtatcaca tgggaaatag aaagacgagg aacaccatct
     7621 aaacttgaat ttacaatagt catggatgat atattagagt tttgtgaagg taactctgta
     7681 agactatatt acaaaggtgt aggtatattc tatggatata tatttcagaa gaaaagagat
     7741 aaagaaaatc acattaaaat tgttgcttac gaccagttaa gatattttaa gaacaaagat
     7801 acttatgtgt atagcaataa aacagcaagt gaacttgtaa aaatgttggc taaagatttt
     7861 aatttaaaat acaatgtcat agaagatact aagtataaac tatctagagt cgaagaaaat
     7921 aaaacactct ttgacatgat actaacagca ctagatgata ctctaagaga gaaaaaagaa
     7981 atgtatacct tgtatgatga ttttggaaga ataacattaa agaatgttgc atcaatgaaa
     8041 ctggatactg tcatgaacaa tgatgtaatt gaggactttg actataattc aagtatagat
     8101 agtgatactt atacaaaaat caaacttgta agagacaacg aggagtcagg aaaaagagat
     8161 gtgtatattg ctcaagactc tacacatatg aggagttggg gaatacttca aatgtttgag
     8221 acagttgata aaaatatgaa tgaagcagag ataaaacaaa agtgtgatat acttctaaaa
     8281 ctatataata agaaaactaa gtcattaagt ttaaaaaatg cacttggaga tattagagtg
     8341 agagcaggtt gtttagtacc tgttttttta aatctaggag atattaaact tcaaaattat
     8401 atgctagttg agaaagtaaa acatactttt gaaaataaca gtcactttat ggatttgact
     8461 cttgttgatg gagatgaatt tgcttcttat tcttcaagct catatagtag tggaaatact
     8521 aataataaaa atgagaaaca aaatggtcct gcacaaagta ctacaagtaa agaagacaat
     8581 gatatgataa ataaattaaa taaagtattt aaaaataagt tatcaaatac aggaaatata
     8641 tttgttaaat attctaatgc ttacaaagtc aatgcagctt taatggctgc tatttctata
     8701 catgaaactg gtaatggaag ttcttcactt tgcaaaaata aaaataattt ctttggtatg
     8761 aaagggatgt cttttagttc tgtagatgaa gggataaaaa aaggtattag caatttatca
     8821 agaaattata tccatacagg aagaaaaaca ttagagcgta ttagagacaa atatgcacct
     8881 ctttatgata gccctcttaa taaagattgg gtaccaggag tgggcaaatt ttataaacaa
     8941 ataacaggaa gtacctatac ttctaataat gcaggtacag gagttggaag taacgaagaa
     9001 gcagaaaaga atttaaaaga tacaacttat caagttcaaa acaataattc taatacatta
     9061 acaaacaata atagtaaagt aagtaaagtt attcaagaag caaaaaatca acttggtaag
     9121 ccatatgctt ggggaggcaa tggtccaaag agttttgact gttctggttt aatggtatgg
     9181 gcatttaaaa gaggtgcagg aataaatcta cctagagttt cagcagacca atcaaaagat
     9241 agtagaggga aactattatg caacataaat gatgtaaaag caggcgattt agtattcttt
     9301 aaaaacgaac aaggaaaagt acatcatgtt ggactatata taggaaatga ccaatatatt
     9361 catgctccac aaactggtga tgtagtaaaa ataagtagtt taagtggtag acaaaagaaa
     9421 aagcatgatt ttgaaagagc tagaagattc ttttaagtga ggtggtaata tgagtcaaga
     9481 attattgcaa ataattaaaa aagctgcaat ggatgcagta gaaacaagta atccaattca
     9541 aattgcattt ggaactatag aaagtgttaa tcctttagta gttaagatag agcaaaaagc
     9601 ctcttttgaa gaatcttttc taatacaaac agatacattc aaaaaatata cagataaaaa
     9661 aataggagat aaattagtct taattcgtat gcaaggagga cagcaatatt tgattttaga
     9721 taggatgtga taaggtgtta ccaagcgata atttagatta tgacattgaa gatgtatcga
     9781 taattaattt tgatgttaga caagaaccaa gtaagacgtt taaattgaat atagagaaaa
     9841 atagagtaga tggtatttgt gatgatgtag aagcattaaa acaaaccatt tttttaattt
     9901 taaatactga aaggtatgag catcttattt attctagaaa ttatggtgtt gaattaaatg
     9961 atttaattgg agaacctatt tcatatgtaa taccagaact tgaaagaagg ataacagaag
    10021 cactaattca agatgatagg attgaaaata tagataattt tgagtttcaa aatataaagg
    10081 gtaaagtaca atgtagattt tcagttcata caaaatatgg aaatataaaa gcagagaagg
    10141 tggtgagtgt ataattgttt gagttaatga catttgaaaa tataattaaa agaatgttag
    10201 atagtgtacc agatactttt gataaaaggg aaggttctat aatatataat gctcttgctc
    10261 ctgttgctat agaacttaca gaaacataca ttgcaatgga tgaattacta gaccaaacat
    10321 tcgtagatac tgctagttat tattacttgg agaaaagatg taaagaacga ggaattacac
    10381 cactatctgc aactaatacc attgcaaaag gagtgtttaa tatagatatt ccacttgatt
    10441 ctaggtttaa tctaggagaa tacaattata ttgcaattga gagaatatct gaaaaaacat
    10501 ataaaatgaa atgtgaaact gctggaccta tatttgagtt aggaaaacta atacctattg
    10561 aatatataga tggtcttgaa actactgaac taactgaaat cttgataaat ggagaagatg
    10621 aagaatctga ggacagttta agacaaagat attatgatag tttaaattct caatcttttg
    10681 ggggtaatat acaaaactat aaggatgaag ttaataaatt gcctgacgtt gggggagtta
    10741 aagtatatcc agtttggaac ggtggaggta ctgttaagtt agtaataatt aattcaaatt
    10801 ttaaagtacc aagtactgat ttagttaatt tagttcaaga agaaattgac ccaaatatga
    10861 aaggagaagg tcttggatta gcaccaattg gacaccgagt cacagttgaa ggagttacaa
    10921 gtacaactat aaatatatca gcagagataa catataagag tggatataca tgggagaata
    10981 taaaatcaat tgcagaagaa gcaatagacg actatttaaa tgaacttaac atgagttggg
    11041 aagatgaaga aaacttaata gtccgtatat ctcaaattga aactagatta cttagtatag
    11101 atggagtgtt agatattaca aacacaatga taaatgaggt taaatctaat ctaacaataa
    11161 atagtaacag tatagtagtg agaggtgagg tagttggata aagagattaa tctaataaat
    11221 tacttaccac aaattctaca agataaagaa gaatatataa aagtatttaa tgtagaaaat
    11281 aaagaaataa aaacactaca tgataaatta aaggacctat caaatgacca gtttttagag
    11341 gacctaacta taagtggtat aaaaagatgg gaaaagataa tgtctataac tcctaaaagt
    11401 aatgagagtt tagaagatag aaggtttagg atttttagta aatatataag taaactacct
    11461 tactcagaga gatttttaag gaactggcta gataatgtag ttggagaagg caattatgag
    11521 ttaactataa ataatgctac ttataatata catcttgaaa gtgatgctag aaatcaagat
    11581 tggtttgagg aagttcattc ttttgtaagt aatattaagc catgtaatat gactttagat
    11641 tacactagag tgcttataag caaagacaat tatatgaatt ttggtataac aaccctaatg
    11701 ggtcaagaaa taactatata cccttggagt ccaccagata tagaaactta tggagaaatt
    11761 gatgtattaa ctggcaatgg agttggatac caagagataa caatatttta ggaggtgata
    11821 tattggctat agataaaagt tattacacta taattacaga tgtaggaaaa gcaaagatag
    11881 caaatgcaag tgtcacaggt aataaagtgg gatttgtaaa aattcaactt ggtgatggag
    11941 gagggagtga atatactcca actgagagtc agacagctct caaaaatgtg gtatgggaag
    12001 gcaatatcgg aaatacaact acagatgaaa ctgcaccaaa ttgtataata ttagagagtt
    12061 taataccatc aagtgtaggc gggtttatga taagagaaat aggatattta gatgatgaaa
    12121 ataatttaat tgccatttct aaatacaaag agtgttataa accttctata gaacaaggtg
    12181 cagtggtaga catgaaggtt aaaactgtgc ttattgtatc taatgtaaat aatatagaac
    12241 ttaaaattga cccaacaata atctttgcaa cactcaaaga tatacaagac ttagaaacta
    12301 aaataggtac tgttaatact aaaattgata caactaaaac agaattaaca agcaacatag
    12361 aaactgctaa aacagagtta aatactaaaa tcgaccaatt aatcgcaggt ggctcaaatg
    12421 ttgcatacac tcaaagggtc gcaattgatg attgggttga ggatgcagaa agtggattca
    12481 aagcaactgt aacacatagt ttattaacac atagaatagt tgtaaatatt atagatgcta
    12541 ctacaaaaga aaatatagtt ccaaatttta aaatagttga tgataattca attgagatta
    12601 gaagtgaagt aaaagttgag ttaaatgtct atgtgataaa tggaaatgca gaaactcatt
    12661 ttataaatgc aactgtagat gataacagag tatctgaaat gactacttat tcatctaaga
    12721 aaatagagga cagattggtt aatatagaag aaaaggtaaa tggtggttta tctaatattg
    12781 caacaagtgt aaatgagttg ataacttatt gttagagagg agagtgagaa aatgcagact
    12841 gaatggaatt ttaattatgc taattatgta caaaatgttt cattgccacc tgggcgatat
    12901 aaattagaat gttggggtgc ttgtggtggt gctgtcgata caagcgattg gactgattgt
    12961 gcaaaaggtg gttattcaaa aggtgagatt gtatttaaaa aaagaactaa tctacaaatt
    13021 tgtgtcggtc aatccggtta tgagaaagtt tctgaaggtt caagccttac tagaagtggt
    13081 tttaacggtg caggcgctgc tggcaaagtt actactggta gctttgctta ttctaaatac
    13141 ggtggtggag caactgatat aagactttat catcctagtg caacttgggg taacactgaa
    13201 agtttgcttt cacgcatact tgttgcaggc ggtggaggag gtatgaaaaa taattttgct
    13261 tctgctcgtt ctattggtca tggtggtggt tatgtaggtg ttaatggagt tggtcgtgac
    13321 agagattttt gtggcggtgg ttctcaatac caaggtggaa caagttacga cacagaagaa
    13381 taccatggtt cattaggaaa aggaggttat ggtaacatag gaataggtgg tggagggggt
    13441 tggtacggtg gtgctggttc ttattctaat gaatgtggag gtggtggaag tggttacgca
    13501 ctaaataaag atagttataa ggcacccgga tatataccaa cacctgaata ttatcttgaa
    13561 aatatagtca tgactactgg aggtaatact actaaagcag atggttatgc taaaataaca
    13621 ttactacaag cattgccatt tttaacagta tcttcttata attctacaca ggcaacattt
    13681 aaagccgacc acacagaccc tgcattactt acaaagatag aatggtttat agatgatgta
    13741 ctaaaagaaa ctataacaac aaatttaaca gaagagaaaa caattaatta tacattagaa
    13801 gataatgcat tacacacact taaaatagtt gttacagaca gtaataatgc tacagcagaa
    13861 aaagtgttaa gtataagtaa gaatataatg ccactgcccg aaaatgtaaa tttaaatgat
    13921 atatcaacaa aattagttga ggttaatgca ggatttaaag ttgggaaaac aagtattata
    13981 aacactttag cacttaaaaa tatagaagca agtttaaata atacacttgt ggagttatca
    14041 gagaaaataa aaacaagttt tgatagttca gacactagtg tgcaagattt acaaaatcaa
    14101 gtaacacaaa aaaacaatac tataactcaa ttagaaacag agttaagtaa aagaaaaaga
    14161 tttataacag gaacttatac attcacaaaa acggatgcag agaattttaa cttaagtata
    14221 tatgacaaag aaggaacttc aaaaactctt actataccag ttaacatggg cttctctcct
    14281 agtttaattg tacttagtgg agtaactttt agtacaacaa gtaaatctta tgtttatttt
    14341 gataatgttt gtaattcaaa tttttataat tttggctata attctgattc tactcatagt
    14401 aacccgaaag ctgttggtat tcttaatgta tctaatgttg ggtattcttc attagtgctt
    14461 acattatata aattatcaat gtcagaagca gttggaatat gggcaaaaga gggagctaca
    14521 ttaacttaca aaatatatat ttaataaaaa aatgaggtga taatatgaac agaaataata
    14581 gaataattta cgaccaaaca ggcaatatat ggcttcaaac tggtgaagca acaggagata
    14641 tacaagagtg gtcaaaaata actgaattaa attttttgga tgttgaattt ggtagtatag
    14701 actatagtaa acaatatata gagtctataa atccagttac aaaagaacca atcatcaaag
    14761 acatagaagt cattttaaca gatgaacaaa agagattaca agcattagaa aaagaattaa
    14821 gcatgttaaa agaagaaaat aaaaatagag atagtgagat agtaaacaca gcctttgagg
    14881 tagaaaatat aaaattaaat aacaatttat aggaggaatt aacatgtata acttattaaa
    14941 acttatgata gaacaaaaga actatagcac taaagaggat ttgcaacaca aaattgatgt
    15001 attctatgca gtaaatagaa ttacagaaga acaatattta gaacttacaa gtttattaaa
    15061 taaagaagaa acaccagcag aaccaataaa ataggttctt tttttattga aagaaggtga
    15121 ctaaatgact tttaaagagt tagttaataa agttagaaat cttgtattag aagcaaagaa
    15181 tgtaactata gaagatacag aaagtaaatt tacaagtgaa aatgtagaag gagcattgaa
    15241 agaatgtata gatagagcag atgaggcttt tcaagaagcc gatagtggaa aaacactttt
    15301 atcaactgct atcggctctc ctgctacatc agaacaaaca tttcaagatt atgcgaacta
    15361 tattacagga tttaagagca atataagcaa tttagaaact caattgaaaa gcaaatattc
    15421 tgttagatat ggtcctattg atggatatga tggtaatcct ttttctgcta attttggcaa
    15481 gagtgcaagt taccttattg tctatgttta ctttagaaga agtgtatatt attataatcc
    15541 cagtggtagt tctttaggaa gtaatacagg aggttctgaa cgtgcatgga ttactataga
    15601 cagcaataaa actggttttt cagttcattc atatgatact agttatgagc catatccttt
    15661 tacaggttat tatattgctt gtttcgcata ataaattata ttattaaaac taggaggatg
    15721 tatggaagaa attagcataa atctattatg tgcagttgca ggagttgtaa tatcctactt
    15781 agcatttaga aatagctcaa acagaaagat acaagatgat acagaaacaa ctacaaaatt
    15841 agaacaacaa ataacttttc tgtgtgagaa tgtaagagat ataaagcatg atgtagcaaa
    15901 gtttaataca agtttcttag atatcagtga acgagttgca aaagtagaag caagtacaaa
    15961 acaagcacat cttagaattg atgaaattat aaatagaatt ggaggaaaat aaaagatgga
    16021 caatttaata agttttatac cagagcagtt gctaatttta gtagctgctc tttatgtttt
    16081 aggtgttgga ttcaagaaat ataaacaatt agataataaa tacattccag tagtgttact
    16141 gatacttgga ataggtttct caatatggat gctaggatta aatcctgttg cagtcttaca
    16201 aggtgtaatt tgttggggag ttgcaatagg tataaatcaa acttacaaac agttgaagga
    16261 tggtgaaaag taatgaaatt aacaaaaata ttattactca caaaaattta aaaaatttca
    16321 ttaaatagag tatactttta tagctaataa gtatataata atagtacaga actcgtaaag
    16381 catttatatg ctcaaatata cgggacattg atttttgaag gaacccgcca agcttctttt
    16441 atagctcaaa tagtcgggac agataaattt acccactaat attaattggt gggttttatt
    16501 atataaaagt atatctatga aaaggtgaaa acaatggttg aagtaaaaga agaaaaacat
    16561 ttgatgaaca aatagatatt ttaaaaagta gaggattaat aataaatgat aaagaagatg
    16621 ctaaatttgt attaagtaat gtcaattatt ataggtttac agcatatctt ctaagtttta
    16681 agaatgatga tggctcatat aaagaaggaa ctacgtttga agaagtttat gatatatata
    16741 ggtttaataa ggaatttagg atattattaa cagatttgtt agggagcata gaaatagcat
    16801 ttaggacata cattggatat acattagcaa ttaaacatgg tgcttgtgga tatctataaa
    16861 gggagagttt caaagatgaa aaattctata ttaatttttt gacagcatta gagagagaaa
    16921 aaaataataa ttcagataag ctttttatta tacaccataa agaaaaatat gaaggaaaac
    16981 ttcccatttg ggttgcaaca gaaataatga cttttggtat gttgtcaaaa ttatattcaa
    17041 acatgttgcc agaagatact agatacataa aaaataattt gtgtagagtg aatactttat
    17101 tagttaaatc ttggttacaa tcattaacac aggtcagaaa tcaatgcgct cattatggta
    17161 gaatatataa taataatttc cgtattataa caataaaaaa cgaatataaa aagtataact
    17221 tggataataa aaagatattt tcttatatac ttgctatgaa gcatttgact atggataaat
    17281 taatttggaa tagttttttt ataaaacttc aaaagttaat taatgattat aataattcta
    17341 tagacttaaa gcttattggt tttcctaata attggataga gatattggct aaataaaata
    17401 gttactttaa gaagtttata aacacttact atatgtaagt gtttttttat tgaaaagaag
    17461 gaggaaaata aataatgaaa atatgtatta cagtaggaca cagtatttta aaaagtggtg
    17521 catgcacttc tgctgatggg gtagttaacg aataccaata caataaattt cttgcaccag
    17581 tattagcaga tacatttaga aaagaaggtc ataaggcaga tgtaataata tgccctgaaa
    17641 agcagtttaa aactaaagca gaagaaaaga tttataaaat acctagagtt aatagtggag
    17701 gatatgattt acttatagaa ctacatctaa atgcaagtga tggtcaagga aaaggttcag
    17761 aagttctata ttatagtaat aaaggtttag aatatgcaac tagaatatgt aataagctag
    17821 gtacagtatt tagaaataga agagctaaat tagataaaag attatatatc ttaaatagtt
    17881 caaagcctac agcagtatta attgaaagtt tcttctgtga taataaagaa gattatgata
    17941 aagctaagaa actaggtcat gaaggtattg ctaagttaat tgtagaaggt gtattaaata
    18001 aaaatataaa taatgaggga gttaaacaga tgtacaaaca tacaattgtt tatgatggag
    18061 aagttgacaa aatccctgca actgtagttg gttggggtta taatgatggg aaaatactga
    18121 tatgtgatat aaaagattat gtacctggcc agacacaaaa tctttatgtc ataggtggtg
    18181 gagcatgtga aaagataagt tctattacta aagaacatta tacaatgata aaaggtaatg
    18241 atagatttga tacactttgt aaagcattag attttattaa tagataaatt aaaaagtagc
    18301 aactaaaagt agttgttatc ttctaatgcc tcattcataa taaaaaaata atttattata
    18361 taataatatt aaagtttgtt tctaaggtta atattatgtg atacaattca agtgggtaaa
    18421 tattatttga atgaggtgtg gggatgaaaa atttaagcaa aaaagcagtt attttatcac
    18481 tttcattggt agttgtatca cctttggtac ataagataaa tgcacaagat attgagaaaa
    18541 atagggaagt tagatatgaa acaaggaaag ctaataactt agaaaatatg acaatggaag
    18601 aagcttttcc agatgaaaat tttagaaaag taatttgtga taaactaagt attttagatg
    18661 atggaaatcc aattggaatt tcacaaaaag cgattataga atctacaaaa ggtttaacct
    18721 tagggagcaa atcaataaaa aatttatctg gaattaatta ttttattgga ttagaaaatt
    18781 ttagttgtag aggaaataat ttaacgagtc tagatttaag tagcaacata aatttaaaag
    18841 aattgtattg taacgaaaat caattaacaa gcctagattt aagtaataat agagagttaa
    18901 caacgttgca ttgtggagaa aataatctag cagacttagt tatagaaaat tctaaattag
    18961 acgaattgaa ctgtagaaaa aataatttaa agagtttaga tataactaaa gcaactaatt
    19021 tgacaactct tttatgtttt gaaaatgaat tgactagttt gaacttatct aagaatcaga
    19081 aattaaaaat tttgaaatgt gattataata atttaaatga tttagatata tctacaaatt
    19141 tagaattaca ggatttaaac tgttctttta atcaattaga aaatataaat ttagataata
    19201 ataaagaact tgttaagtta atttgtcaca acaataaatt gcaaaattta aatttaaaaa
    19261 atcatgaaaa acttgtaaaa ctatattgta atcaagaaca attaagtact ttaaatgtgg
    19321 aaaattgtat taatttggaa gatttaactt gtagtagaag taatctgaag aatttggata
    19381 taagtagcaa taagaattta aaatatctag agtgtgtagc aaacaagtta acaaatttga
    19441 atatagagaa taatatagaa cttttagaat tatactgtta tgacaatgaa ataaaagctt
    19501 tagatataag taaaaatatt gacttagaaa atttaacttg ttataaaaat aaattagata
    19561 gtttaaatac aagtaaaaat aaaaatctta aatacttata ttgttcacaa aatgaattaa
    19621 ctagtttgga tgtaacaaaa aatacagaac ttgtaaggct atattgtggg gaaaatagat
    19681 taaccagctt agatgtaagc aaaaacacaa aattaaaaga attagattgg tcaaatcaaa
    19741 aaaaatcaac atcaacagga ggttcgtcag ggagtggagg aggttcatta tctacaaatg
    19801 aagagtcctc tgaacctact tcaacaccat ctaaggaaaa attaacaggt gcagacagaa
    19861 atgaaacatc agtgaaaata agtcaaaaag gatggaataa agctgataat atagtgttaa
    19921 taaatgactc tagcatatcg gatgctttat cagcaacacc atttgctaag tctaaagatg
    19981 caccaatatt acttactaaa aataataact taaataaatt aacagaaaaa gaaataaata
    20041 gattagaagc aaaaaatgtg tacatagtgg gtggattaaa gtctgttgat gaaaaagtag
    20101 tgtctgattt aaagaaaaaa ggattaaatg taattagaat atctggaaat gatagatatg
    20161 aaacatcaat aaaacttgca aaagaattag acaaaaattc taacctgtca aaagtagtag
    20221 tggtaaatgg agaaaaggga ctagctgatg cagtaagtat gggagctata tcagcaaaag
    20281 aggaaatgcc aattcttctt actaatcaaa atgatgatat gaaagatata aaagacttaa
    20341 tagctaataa aaacatatca aaatcatatg taataggtgg agaatcttta tttaataata
    20401 aagaagtgaa taacacatta ccatctgtaa ctaaaatagc aggttctgat agaacagaaa
    20461 ctaactcaaa agtaataagt catttctata gcaaagatac acttaatgac ttatatgtag
    20521 ctaagaatgg aatgaataaa caagacgatt tagtagatgc cttatcagta ggagtgcttg
    20581 caggtaaaac tgaatctcca gtagtattag ttggaaatgg attggataat agccaaaaag
    20641 agttcatcaa gaataaaaag tttaaaaata taactcaaat tggtggaaat ggaaatgaaa
    20701 aagcatttaa tgaagtagaa aacttagtta aatagattga aaggtagtaa ctggaaatag
    20761 ttactacctc ctttttttat tcattttcag ttttttcaat aattattttt ccatcctcaa
    20821 aagtaacaag aacatccctt tcatcaggag agatattcat ctctttaatc catgacattg
    20881 gaagtatcaa tctaggtgta aaagagcctt tgccactttt attaaatcca attctaagtt
    20941 ttctttgctc caataatatc attcctttct actattaata aatgccagca ataataaagt
    21001 aaataccaaa gcaataagag ctttaaaagt aaaacttatt attatattaa taatatttaa
    21061 aactatagtg ataccaagaa taatcataca aattctataa aatttattat tcatattaaa
    21121 ttagaaaatg tgttataata ttagaaagaa gctacctaga aggaaggtat tttcctccta
    21181 ggattgaaac ttataattct ttaatcaatc ttagtattgc gagtactaga ttaattaaaa
    21241 ctaatgtgaa agagaaagca tttttaattt tattaaattt gttttctctt ttattttttc
    21301 ttctttctaa tctccccact ttcttacctc ctttctatat ttatattata tcatcacgtg
    21361 acgtgaaagt caataaatat tccatatttt tcaataaagt tgtaaaatat attaatataa
    21421 aatacaaaat ataataaaaa taagtggatg tttacattat acacccactt attttagagt
    21481 tattcctcat cagatttttt tattataatc tcatcttcct taaaaataac tacagcctta
    21541 cgtttttccc ttgtaaatcc cattttttca agttggctta aaggtaaaga aattttagcc
    21601 gaataagtac ctgaaccacc tttactgtaa actattttag attctttttg ctccaaacta
    21661 ttttcctcct tttttatatt gtaatgtaac aaatattaac aataaaatgc ttactattaa
    21721 tcctattata ttgttgatat taaaatgtaa aaataaaagt attatgtcta ttactatagt
    21781 tatagcaact ataataagac agaatttata aactttatca ctcatatttt atagaaaatg
    21841 tgttataata tttagaaaga agtcactagg aagaagatta aattcttcct agcttacaaa
    21901 actataaatc ttttaataat ttaattagtt tatcaattaa atttattaaa atgggtaaga
    21961 tagagagagc agttttaact ttggcgagtt tgctttctct atcttttttt cttctttcta
    22021 ttctactcat tttccttttc accccctttc tatatttata ttataatatg ccgaaccggc
    22081 aaaagcaata tttatttcca tatttttcaa taaatttata ttgacaaact atattttttt
    22141 acaaataaaa ataagggtgg caaaatcaaa cttgccaccc ttttgccacc gaaaataaat
    22201 aatagaataa atttaaatta aacataataa aacataaaca acaaaactga ccttaataaa
    22261 acattgaaat accaacactt gtaaaaaata acaacaataa acaaataacc tagaataata
    22321 ttacaactta agtaaataat aattttgaat aataatttac ggaggtattc atatgtttaa
    22381 acacgataaa gcattattaa aagaagtaaa agtagaaaga ccaaatcctc aatatgctgt
    22441 tttaatgcaa gaacagttgg gtggtgcaaa tggagagtta aaagcagcta tgcaatactt
    22501 gtcccaaagt tttagaataa aagaccctca aataaaagat ttatttcttg atatagcagc
    22561 agaagagctt agccatatgg aaatggtagc acaaactata aatttattaa atggacatga
    22621 tgttgattat aatgcagtta atacaggcga aatagaaaca catgtattaa ctggtctatc
    22681 accagtctta ataaactcat caggagctcc ttggactgct aattacgtta cagttacagg
    22741 ggatttagta gctgacttac tttcaaacat agcttctgaa caaagagcta aggtagttta
    22801 tgagtacttg tatagacaaa tagatgataa atatgtaaaa gaaacaatag atttcttact
    22861 taatagagaa gaagcacata atgctctatt tagagatgct ttaaataaag ttaaagatac
    22921 aggttcaaat aaagattttg gagttacaga agattctaag ttatatttcg atttgtctac
    22981 tccagggcca aatcacgata ctaagataga tattaatcct ccttcttttg aaaaaccttt
    23041 aaaaaaataa tttgatattt tttatgttgt tttgttttag tagttataaa aattatttac
    23101 tagaagaatt aattatatga aaaattaaat atgtatagtt aaaaatgggt atacacttat
    23161 tataagtgta tatctatttg tttattgcaa aatatataat aaaataacaa atgaggtggt
    23221 attttggaca gtaatagaaa acctaataat ctaataaatg aaaaatctcc atatctttta
    23281 cagcatgcat ataatcctat aaactggtat agttggaatg atgaggcttt taaaaaagca
    23341 aaagaagagg ataagccaat atttttaagt gtaggatatt caacatgtca ttggtgccat
    23401 gttatggaaa aagaatcttt tgaagatgaa gaagttgctg aaataatgaa tagaaacttt
    23461 gtggcgataa aagtagataa agaggaaaga cctgatgtag atagcgtgta tatgacagtt
    23521 tgccaagcca tgacaggtag tggtggatgg ccaatgacca taataatgac accagataaa
    23581 aaaccttttt ttgcaggaac atatttccca aaatattcaa ggtataatag gcctggagtt
    23641 atagatttat tagagaatgt atctgaaaaa tggaatacaa gcagggatat attaataaaa
    23701 tctggagatg aaataataaa agctcttaag gatgattttg atgtaaaaaa tactgagggc
    23761 gatttatcaa aagagatgtt gagttcatca gttagagtat ttaaggctat ttatgacgaa
    23821 aagtatggtg gttttggtaa tgctcctaaa tttccaagtc ctcagaatct tatgtttttg
    23881 atgaaatatt atagtataga aaaagataaa gatgtgttaa aaatggtgga aaaaacttta
    23941 gatggaatgt atagaggagg cttgtttgat catattggat ttgggttttc aagatactct
    24001 actgataaaa agtggttggc tcctcatttt gaaaaaatgc tatatgataa tgctatgctt
    24061 actatagctt ttttagatgc gtataaaata acaaagaaag agttatataa agaaattgct
    24121 ataaaaacta tagattatgt agttagagag atgaaggata aagacggtgg gttttattca
    24181 gcacaggatg ctgatagtga aggtgaagaa ggtaaatttt atatatttaa tcctcttgag
    24241 ataattgaag ttttgggtga agaagatgga actttcttta ataattattt tgatataact
    24301 agtagtggaa attttgaagg caaaagtatt cctaatctta taaaaaataa agaatatgag
    24361 agacataatg aaaagatagc tgaccttagt gaaaaggtat ttgaatatag aaaagaaaga
    24421 acatctttgc ataaagatga caagatactt acatcatgga atgcattgat gatagttgct
    24481 ttaacaaaag catatagtac tttagaaaat gatatatatt tagagtattc aaataagtgt
    24541 cttgatttta taaataataa tcttgtaaat gagtcaggta gattattagc tagatataga
    24601 gatggaagtt ccgattattt agcatattta gatgattatg cttttttaat ctgggcgtat
    24661 atagagcttt atgagtcaac atttaatatg aaatatttag aaaaggcttt aaatttgaat
    24721 gaaaactgta tcaacctttt ttgggattat gaaaaaagtg ggttttatat atatggtaaa
    24781 gatagtgaaa atttaatagc aagaccaaaa gatttgtatg atggagctat tccatcagga
    24841 aattctgttc aattatacaa tcttataaga cttgcgaaaa ttactggaga tagtagactt
    24901 gaagaaatgt catataaaca attaaaacta tatgtggata atgtaaagag ttctccaact
    24961 ggctacagct tttatatgct ttcattgatg tttgaacttt attctactaa ggaaattata
    25021 tgtattttta aggaagattc tgatttaatt gcatttaaag aattgataag tgaaaatttt
    25081 ataccaaatg caactttttt agcaaagaaa tacaatgaag aaaacactat tataagtttt
    25141 ttaaataact atagattgaa ggatgataag actagttatt atgtgtgtca aagtaatagt
    25201 tgtagccaac caattaatga tttacaaaaa ttaaaagata tgattttagg agtagaataa
    25261 ccataatagt gaaaaaggag atgctttaaa ataaaagtct aatcatctcc ttttttgtac
    25321 aaaaaacaga aaaagagttc ctaagaacac ctaatctatg taatgattgt tttgttaaaa
    25381 aataaaaatg acattatagt tatataaaat attgcaccat aaaaaattag tattattata
    25441 gatgctatag tattaatcgt ttttatttac cctatttctt ctttttgcaa attcttcaag
    25501 tgcttcagat ttaattttat ctatttcttc atattccata aaattagcat cttcttcaaa
    25561 tccatcttgt tcttcaccat taaaacgata catttttttt agtaaattcc ataatgtctc
    25621 caattcatct ttggtaaatt cgtgaaatac atctgccatg aagtttattc cagtcctatt
    25681 acatgttacc ataacctttt ttcctaagtc tgtaactcta acattaattg ctctcttatc
    25741 atgaggactt ggaactacat caacatatcc atttttttca agattggcaa ctagtctatt
    25801 tatattttgt ttacttgtac ccatttttct tgcaatatta tttaatgttg tttcttcctc
    25861 tggtaaatga agaattgata aaatagtcat atactgtctt gaagtaagta taccaaagta
    25921 tttatctcct tctatttgta ttttgtttgt aagggtaaaa agagttgcat atgtttgatt
    25981 cattaaaaag agctctttta attcatttga ataatccatg aaatatatct cctttatttt
    26041 tagatgttgt tttgtcgaaa acaggatatc atgaattaat tctaatgtca atatggtgtc
    26101 aaaatatatt aaagtattac ttttttatg
//
LOCUS       DAEPXF010000041        25645 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain HB27
            SAMN13376389-rid12479423.denovo.043, whole genome shotgun sequence.
ACCESSION   DAEPXF010000041 DAEPXF010000000
VERSION     DAEPXF010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13376389
            Sequence Read Archive: SRR10518432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 25645)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25645)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001069624.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001069624.1
            Genome Coverage       :: 315x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/15/2021 01:54:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,165
            CDSs (total)                      :: 4,076
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 89
            rRNAs                             :: 10, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 10 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 19 of 69
            Pseudo Genes (incomplete)         :: 44 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 10 of 69
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25645
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13376389-rid12479423.denovo.043"
                     /strain="HB27"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="China: Hebei"
                     /collection_date="2017"
     gene            complement(234..5315)
                     /locus_tag="KRL67_001477"
                     /pseudo
     CDS             complement(234..5315)
                     /locus_tag="KRL67_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013725947.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
     gene            complement(5326..6858)
                     /gene="purH"
                     /locus_tag="KRL67_001478"
     CDS             complement(5326..6858)
                     /gene="purH"
                     /locus_tag="KRL67_001478"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HBH1522367.1"
                     /translation="MSKRALISVTDKTGVVEFAKELNKLDYEIISTGNTFKTLKENGV
                     NVMQVEDVTNFPEILDGRVKTLNPYIHGGILYKRDKESHVETVNEHKIHSIDLVAVNL
                     YDFEGTLKAGKSHDEIIENIDIGGPSMIRSAAKNYKDVIVVVDIKDYDSIIEKLKTDT
                     MTLEDRKKLSYKAFSTTGRYDALISSYFAGEVGDTYPDILNLTFQKEQTLRYGENPHQ
                     NGFLYSQSNAKNPILNYEQLGGKELSFNNLNDLHGCLEVMREFKDSEEVVSVAIKHAN
                     SCGVGLGKDAFEAYTKCYEADKVSIFGGIVGITSTIDKATAEKLNEIFLEIVVAYDFE
                     PEALEILKQKKNLRILKLAKIENSLQPYEMKYLDGKLLIQDRNNILAEKSENVTKAKP
                     TDAQLKDMEFGMRVVKNMKSNAIAIVKNGQTLALGCGQTSRIWALKNALENNKDKDFT
                     GAVLASDAFFPFDDCVTLAHEYGISAVVQPGGSIKDKDSIEACDKYDMVMVFTGIRHF
                     KH"
     gene            complement(6851..7444)
                     /locus_tag="KRL67_001479"
     CDS             complement(6851..7444)
                     /locus_tag="KRL67_001479"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HBH1522368.1"
                     /translation="MLNIGVLISGGGTNLQAVIDGTESGEIKGQVKVVISSKQGAYGL
                     ERAKNHNIKAICETDEDKIIEILKGNKIDLVVLAGYLKIISPKLVNEFRNKMINIHPS
                     LIPSFCGAGFYGEKVHQGVIDYGAKVTGATVHFVDEGADTGPIIMQDVVKVNQDDDAK
                     TLAKRVLEVEHRILKESISLFCENKLKLQGRRVFINE"
     gene            complement(7438..8514)
                     /locus_tag="KRL67_001480"
     CDS             complement(7438..8514)
                     /locus_tag="KRL67_001480"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HBH1522369.1"
                     /translation="MLTYKESGVDIDEGNRAVDLIKGKIKGTYDGNVVGDLGNFSGLY
                     SLKDFVGMKEPVLLASTDGVGTKLKIAQMMDKHDTVGIDLVAMCVNDLICQGAKPLFF
                     LDYIALGKLVPEHIEKIVGGIADGCKMSGCALIGGETAEMPGMYGEDDYDLAGFSVGI
                     ADKEKIVSGNNVKSGDVLVGISSSGVHSNGFSFIRKIFLETYNYKMEQYVEELGMTVG
                     EALLTPTKIYVKLALDVLAKHDIKAIAHITGGGLIENITRVIPKGLGLDINKKSWEKP
                     PIFKMIEGFNAVDERELHKSFNMGIGLVLIVDKENADDVVNFINNRENDNAAYVDKKY
                     SELLEDKAYIIGEVVDSHEGVELC"
     gene            complement(8508..9875)
                     /gene="purF"
                     /locus_tag="KRL67_001481"
     CDS             complement(8508..9875)
                     /gene="purF"
                     /locus_tag="KRL67_001481"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HBH1522370.1"
                     /translation="MCGVLGIYSNKDVTKELYYSLYSMQHRGQESCGLALLDDGEIKY
                     KKDMGLVGDVFKENELSKLKGNIGIGHVRYSTAGGSHVSNCQPLVGSCRKRQLAIAHN
                     GNLVNANYLKDMLEEDGYMFQTNSDTEVILYILARYYKGDIVESLKVTMDYIKGAYAL
                     VIMSQEELVAVRDPHGFRPLVLGKKGDEYIFASENCAIDILGGEVIRDVEPGEIIVVK
                     DGELKSYFYSENYKPVKKSCIFEHIYFARNDATIDNVNAYEFRIKCGERLAQNETVKA
                     DMVVPVPDSGWPGAIGYANASGLKISEGLVKNRYVGRTFIKPTQEEREIAVKIKLNPL
                     STIIKGKSIILVDDSIVRGTTSKQLVKSLREAGAKEIHLRITSPPVAYSCYYGIDTPN
                     RSKLIASSNNVEEMREYIGCDSLKFLDIEGMLDATEHKSTFCKACFDGEYPVKKIDKE
                     ELLSC"
     gene            complement(9887..10585)
                     /locus_tag="KRL67_001482"
     CDS             complement(9887..10585)
                     /locus_tag="KRL67_001482"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007285750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HBH1522371.1"
                     /translation="MLLYEGKAKQVYSTDNENEYVVYYKDDATAFNGEKKAEISSKGI
                     LNNKISTIIFEMLKENNINTHFIKSLSDREMLVKKVEILPLEVIVRNIAAGSICKRVG
                     LEEGVVFDEPIFEISYKNDAYGDPMLNDDYAVAMKLATREELKFLREETLKINELLKA
                     FFLKLNLKLVDFKIEFGKDSEGNIILADEVSPDTCRLWDVNTNEKLDKDRFRKDLGDL
                     VEGYTEVLSRMNNK"
     gene            complement(10591..11067)
                     /gene="purE"
                     /locus_tag="KRL67_001483"
     CDS             complement(10591..11067)
                     /gene="purE"
                     /locus_tag="KRL67_001483"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HBH1522372.1"
                     /translation="MKVAVVMGSKSDYPKLEEGIKLLEKYGIEVVARALSAHRTPEQL
                     SIFLKEIEDDTDVIIAAAGKAAHLPGVIASQTLIPVIGLPIKSSTMDGLDSLLSIVQM
                     PKGIPVATVTIDLGLNAALLALQIMTLKYPKLKEDLKSYREEMAQKVLEDDKNLRG"
     gene            11517..12029
                     /locus_tag="KRL67_001484"
     CDS             11517..12029
                     /locus_tag="KRL67_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HBH1522373.1"
                     /translation="MLEVIKNRRSIRTYKDKNIEEEKITEILKSAMQAPSSKNAQPWE
                     FIIVDDKELLKELSKSQHRAKHVEFAPLCIVVLGNRDKFLKPGKWIQDLGACTQNLLL
                     EVTNQGLAACWAGVFPKNKVVNKVRQTLDLPVKLVPYALISIGYSEEKNEFIDRFDKN
                     KIHRNVYKNR"
     gene            12573..12788
                     /locus_tag="KRL67_001485"
     CDS             12573..12788
                     /locus_tag="KRL67_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH1522374.1"
                     /translation="MRILSETEKISLAAVIKMESDGLLMQRAINMLISDEDLKRQSES
                     SILATEGRIKAIQQFIVENEILISEEV"
     gene            12794..13087
                     /locus_tag="KRL67_001486"
     CDS             12794..13087
                     /locus_tag="KRL67_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore coat protein"
                     /protein_id="HBH1522375.1"
                     /translation="MRNMIENLIKNNTYIDDKVIMLSMLSSAKASANMYLNSALTSST
                     PELRAIYSASLTQMVEGHTALTELSINKGWVKPYDKPIQQLTCSYKESQNVID"
     gene            complement(13282..13998)
                     /locus_tag="KRL67_001487"
     CDS             complement(13282..13998)
                     /locus_tag="KRL67_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HBH1522376.1"
                     /translation="MINLIFDVDDTLYNQLTPFYTAYNKVFSSIKDISIEDLYMSSRK
                     YSDEVFHMTENGEMPIKEMHIYRIMKAFEELGNSITEKDAQSFQDEYIYQQSQITLIP
                     EVEWVLNFSKERNINLGIITNGPSTHQRMKLKQLNIENWVDKSNIFISSEVGFSKPDA
                     NIFRVAENVMNLDRENTYYVGDSYRNDVLGAKKAGWKSIWLNHRGHEVEDLFYKPDFV
                     ILEYKDLMSLFIKICSYKNM"
     gene            complement(14086..15351)
                     /locus_tag="KRL67_001488"
     CDS             complement(14086..15351)
                     /locus_tag="KRL67_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II D-tagatose-bisphosphate aldolase,
                     non-catalytic subunit"
                     /protein_id="HBH1522377.1"
                     /translation="MKKLPIKTVVQRLLDLQEEGKSATLLGIGPMSPNLLQASFELAK
                     DDDFPLMFIASRNQVDADELGGGYVNGWNQETFTKDIKKIADKVGFDGLYYLCRDHGG
                     PWQRDKERNDHLPVDEAMELGKKSYLADIEAGFDLLMIDPTKDPFEIGKIIPLDVVLE
                     RTVELIEYCENERKRLNLPDIGYEVGTEETNGGLTSIETYETFITRLKVELDKRGLPM
                     PTFIVGQTGTLTRKTEQVGTFNFRNAYDLAQMAKKYGVGLKEHNGDYLDDVTLLEHIP
                     SQIIATNVAPQYGTEETRAYLKLAEVECKLEKEGLVDKTSNIRHVLLVNAIECGRWRK
                     WVIGEQKNLTTEEIFKNEVLSNEILDIAGHYTFNNDDVKKEIEVLYANLSKNNIDGQR
                     FVVDHIKRPLRDYAECYNLKGVTARILNK"
     gene            complement(15455..15763)
                     /locus_tag="KRL67_001489"
     CDS             complement(15455..15763)
                     /locus_tag="KRL67_001489"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIB"
                     /protein_id="HBH1522378.1"
                     /translation="MKIVAVTSCPSGVAHTYMSAESLELSAKKFGIEIKVETQGSSGI
                     DNELSLKDIDEATCVILTNDVEIRNMERFKGKKVVRMSVSDIIKKSDALIKKIKDTFQ
                     "
     gene            complement(15830..16885)
                     /locus_tag="KRL67_001490"
     CDS             complement(15830..16885)
                     /locus_tag="KRL67_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit EIIC"
                     /protein_id="HBH1522379.1"
                     /translation="MRILKFLQKHLMTATSYMIPFVVAGGILFALSVTLSGQAAVPET
                     GWLAKLNQIGAAGLALFIPILGGYIAFSMADKPGLAPGMIGAYLAKEVNAGFIGGIIA
                     GFIAGFVVLQLKKIKLAPTMRTLGSIFIYPLIGTLITGGIIVFLIGEPIASFMTWMTN
                     WLNGMSGVSKIPLGGILGGMIASDMGGPINKVAATFAQTQVDTLPYLMGGVGVAICIP
                     PIGLGLATLLFPKKFSKEERDSGKASLLMGCVGITEGAIPFATSDPVRVIPCIMAGSI
                     IGNIMAFLLGCLNHAPWGGLIVLPVVDNRLGYIASVLTGAVVVAVLMKLVKKDVKEDE
                     EIEEDLDDSIELIFEEL"
     gene            complement(16887..17339)
                     /locus_tag="KRL67_001491"
     CDS             complement(16887..17339)
                     /locus_tag="KRL67_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBH1522380.1"
                     /translation="MSKLVNVKCSFKIHVNSKEEAIISLVDVVSKEGYLIDKNQFLED
                     VLKREETLSTYIGHGIGLPHSQSVGVKNSCITIGKLDTPIEWTEEGEKVDLIFLISVT
                     KDNENNLHLKILSKLARLLMHESFRNQIRCSDEQTVYNLIKEKIEEED"
     gene            complement(17336..19366)
                     /locus_tag="KRL67_001492"
     CDS             complement(17336..19366)
                     /locus_tag="KRL67_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBH1522381.1"
                     /translation="MISGRMLLIMNFLKGKNKTSYKDISKQLSIEERKVRYDINNLNI
                     VLRSLNKSVIQKMNKGVLIIPDDFQVVCINNNEYVFSLTERVSIMKIISMFKIDNLNL
                     EKLSKEFQVSRSSIKNDLNVLSSELERNQITITYDKAFKISGEEDIIYKQRLNILKSY
                     SYLFGKEKSDLSVFEKYLVNVIEKIFKGIYLIDIYNWSMNLLLQMGWTLNDESFTWYV
                     SNIFLFTWYIHSDEKHPLESASLSEPCFENKFDQEFETIIQKKLTKEQIFLLKSFVFF
                     TNKYASLNEDMDLISTEIIVQDLIYNMSEILEISFKEDMILYKGLLNHIAPLIERIKQ
                     NIQIYEELLHVIPDKYQYVLEATKVSIQRNRVLSQIKNENETILLAIHFLGSIHRNER
                     NNYKNILLVCGFGYGVIAMLKDSLVNDYQINIVESIPSYKLQDYKDWESIDFIITTSK
                     IVIDLPKTIIEINPFLQEEDYLKFEEKGIKRKNVLTNYMSIKKRLDFLEEDAQKRVLS
                     IICQELGYSDERILFRQLNLSDLIGIDTIRIIDEEMKWEDAVKISSNILANCDFVSAQ
                     YAENIIDILKNVGFYAVKDNEFALLHGNDSSLVKVSSISLLICKEAVSFRDKKVKIIF
                     CLASRDKKEQIPAIINLTKMVYKTNFIAMLESAKTKEEAEVLIHRYEKEVTV"
     gene            complement(19624..21858)
                     /locus_tag="KRL67_001493"
     CDS             complement(19624..21858)
                     /locus_tag="KRL67_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HBH1522382.1"
                     /translation="MKLSRYKKNSLVLSIILCSLIISILVITVFNTVKLHNISNKQSK
                     NYLNDVSTQIVMNVDSKIKFILSDLRIMADFIKQYEGDSRYEYLSKRKTSYKYYDIGI
                     IDLEGKAEFLSGKKFDLKNTDSYKKGIKGKEYSEIIESMGFVLYSVPIFNDNHEVSSI
                     LIGVSDKESMNEILNIDNFNGEGTIEIINSKGKPLFIGKNTELIKDLSHKYKGSGDEP
                     WAQKMLNDFKNDKSGNITITSSKGVQCLLTYHSITRGLNDWHFLLIVPEDAVLGELNK
                     LNSFTIIMTFIITCIIGIITLILYIIRRKYVNQIENIAYLDSITNGINSTKFSMLVKP
                     LISESPDSTYMMVAINIKDFKLINDCFGSEKGNMTLKHLYNVLYKNIDENEEFVCRHD
                     ADLFYLLIKNRPVLEALELLYKIEADTNYFNKNRENPYFLRISVGIYTIENHNEDLIT
                     IQDHANTARKSFNKTHQSDFSFYSDIERKRLISEKEISNLMEKALENKEFFMCLQPKI
                     DIKSGKISGAESLVRWKNSEKGIIYPSDFIPLFEKSGFICKLDLYILEETCKLISKWI
                     KEGKEVLRISVNVSRQHLNDKLFLEKYKLICNKYNVPTYMIDLELTESIFLENPEAVD
                     VIDDIHSNGFKCSIDDFGFGYSSLGILKDFKVDIIKLDRSFFVSKNNIDRGKVVIKSI
                     IELSKRLGMKVTAEGIEELEQVEFLKNVGCDYIQGYVFSKPLLISDFENFAYENNKIK
                     NIYY"
     gene            22177..25605
                     /gene="uvrA"
                     /locus_tag="KRL67_001494"
     CDS             22177..25605
                     /gene="uvrA"
                     /locus_tag="KRL67_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HBH1522383.1"
                     /translation="MKINNYKNQSIITNPKKFENKYQDLPKTPVELLKVVQSLVIHGD
                     QGKLYGISFNKQQSDEELLRTVPQMLKRIFEINSNPLTIPRNPKQRLVGMCRDYSLLL
                     VSLLRYRGFEARMRAGFANYFESELTYEDHWLVEYYDTLTKRWIKIDAQIDDIQKNYF
                     QINFDTHDVGKTDGFLTGSEAWIRCRQGHAHPDDFGYNKNWKGWPSVKGNLLHDFNNM
                     IGLELLPWDLWTELSSKKYNQLTRAEKNLLDEMAEILSSGNIKIEDLNLLIEKLPEDY
                     LKSIFSQLKILGISEIKELGNPLELEKKFKFTKAINKSIKNSFCHNKNSIYLKGGRQN
                     NLKDIEVTIPKNKITVITGVSGSGKSSLAFDTIYEEGKRCYFENLGNGAKLSEQLQKP
                     EFDLLQGLTPTIAIEQKKGSQNPRSTVGTLTSIWDYLRMLFVSIGKSYCPYCKTPLEK
                     KNNTKNYCPHCQTIFSKINTSTFNANSHTGACHDCNGLGFTYQVNPQLIVKDPTISIL
                     DGATYYFGKLRGKKPNGNWMVGELYAIAKDKNIDLDIPWNELPRDFIDAILYGTDDKI
                     YEFSFESRGRESKIRRPASGAINHIQRLFRESSSENNTLHQYMNKIPCNTCGGELLCI
                     EARFTTIKGYRFPELTKMTIEQLWNWLCELPNQLQQNELSLVNDILTELKIRISYLLK
                     VGLSYLSTDRTAPTLSGGELQRVRLSSQLGSELVGLTYILDEPSIGLHPRDHNLIIKM
                     IEELRDKGNTVIVVEHDKDTILSADYIIDVGPNAGTKGGFIIAEGTTQEIIKNPNSIT
                     GKYLSTYNKIISQNKIIPSKWLSLKGCHANNLKNIDVEIPLNCMCSITGVSGSGKSSL
                     VFHSLLPALEEKLKQKSIPNKNYTKLTGFDAIDDFILMDQTPIGKSSRSTPATYINIF
                     DEIRSLFAETPQAKQKLLDESYFSFNSKKGQCPNCQGLGKTKIILQYMADQWVTCNEC
                     QGKRYQKEILSIQYKGKTITDILDMEVAEAKTFFSDCSDIYRKLSLLDEVGLGYLKLG
                     QNTLGLSGGESQRIKLAKELGTKTKKRMLYILDEPTTGLHFKDIENLLITFRKLVNEQ
                     HSLLIIEHNTEIIRVSDWIIDIGPDSGINGGKIVASGTPDEIKINPNSITGHFI"
BASE COUNT         8403 a         4537 c         2903 g         9802 t
ORIGIN      
        1 aattgactac tagacctttt ttattaatac tttcctttat tatgattact attaataaat
       61 aatcaatttt aattaattat aacacagcgt atattttaat ttaagctgaa ctattattta
      121 ttatataaat aaaaaagaga tgtaatatta aatgattatc atcacattat tacatctctt
      181 caaatctaaa tatctaaaat atatattcta gctatcaatt aaacttcaaa gttctatcta
      241 aaatatttaa ctccagattc aaatatcttt tggtcttttt cacctataat attcttaaat
      301 acttcctcac caattctctc agaatgtccc attttaccaa acactctacc atcagcacta
      361 gttatacctt caacagcata gaatgaacca tttggattaa attcaatatc ataagtagct
      421 tcattattaa agtcaacata ctgagttgct acttgtccat tagcaattaa ttctctcata
      481 acatcttcac tagcaacaaa tttaccttct ccatgagaaa ttgctatatt gtgaatatct
      541 ccaacattag tttgagctaa ccaaggagat ttattagatg aaattctagt tctagcaatc
      601 ttagcttgat ggcgacctat attattgtaa gtaagtgttg gagcactttc acttggaact
      661 cttatttctc catatggaac taatcctaat ttaatcaata cttggaatcc attacagata
      721 cctaacatta gtccatcttt ttgagttaaa aattcattta tagcttcttg cactctagga
      781 tttctaaata cagtagctat aaatttacca gaaccatctg gctcatcacc agcactaaat
      841 ccacctggta acattattat ttgagaagat tttatttcgt ttactattgt atctatagaa
      901 tcttctatgt ctttataagt taaattcttg aatactttta tgtttgcatt agctcctgct
      961 ctttcaaatg ctcttgcaga gtcatattca cagtttgttc cagggaatgc tggtataaat
     1021 actcttggtg tagctatgct taatgatgat tttttagctt ctccttgtga tatataactt
     1081 atagtttcta tctttgattt tacttcttct gttttagttg ggaatatagg ctctaaagta
     1141 ctacaatgtg ctttatataa ttcatctaat gcaagttctt cacctttgat aaatatacta
     1201 gcttctttaa ctgtacttcc aagcacaaca taattgtatc cttctaacat acttaaatca
     1261 tcatttgcta attcaagtac tatatttcca taactagcct caaataattt ctcatctata
     1321 aatgcttttt cagcttcttg actaaactta aatcctattt tattaccaaa agccatttta
     1381 cttattgctt cacctacacc tgcaaatcct aatgcatatg ttgataaaac tttatttcca
     1441 tgtataagtt ctcttacttt gtctaagttt ctctttaatt cctcaaaatc tacaacatca
     1501 ttttctgcct tgcttacaca taacattact acttttgagt ctgctttttt aaattctgga
     1561 gatactactt tcttcgcatc tactgtatca acagcaaatg aaactaatgt tggtggaact
     1621 tcaatatctt taaatgtacc agacatacta tcttttccac ctatagcagg tatctcaaat
     1681 ttactttgtg cataaaatgc tcctaaaagt gctgagaatg gtttacccca tttttctgga
     1741 ttattgccaa gtttttcaaa atactcttgg aatgtaagtc ttgttgtact ataatttcct
     1801 ccaatagcaa ccaatttaca cacagattct actactgcat ataaagcacc atggaatgga
     1861 ctccattttc ctacctttgg attgtatcca taagtcatta ttgtagaagt attagtttct
     1921 cctccaagta ctggtatctt agcaaccata ccttgtgttg gagtcgcttg atactttcct
     1981 ccaaatggca taagtactgt attaccacca attgtattat caaacatctc tactaatcct
     2041 ttttgagaac aaacattaag gtcagataat acttttgtaa acttgtcttt catacttgca
     2101 cacttaacat cttttactat ttcattagaa ctaaaaaatg tattttcttc atctacttta
     2161 gtaacatgga catcagtagt ttgttttact ccgtttgtat ctaagaactc tctatttata
     2221 tctacaattg cttttccatt ccagaacatt cttaaatatc ctgtatctgt tactgtagct
     2281 acatgtgtag cttctaagtt ttcttctact gctaattgta taaatttatc tttattttct
     2341 ttttttatag ctactgccat acgttcttgt gactctgaaa ttgcaagctc agttccatca
     2401 agtccatcat attttttagg cactaagtct aagtttatat ctaagctatc agcaatctct
     2461 cctatagcaa cacaaactcc acctgctcca aaatcattac atctttttat catttgagca
     2521 acttctttat ttctaaagaa tctttgtatt tttctttcgt taggagcatc tcctttttga
     2581 acttctgcac tacaagttaa gattgactct tcactatgtt ctttagatga accagttgct
     2641 cctccacatc catctcttcc tgtttttcct ccaagaagaa ctattacatc tcctgcttca
     2701 ggtctttctc ttactacatt ctcttttggt gctgctgcta taactgcacc tatttccatt
     2761 ctctttgcaa caaagttttc atcatatact tctgcaactt gtccagttgt aagacctatt
     2821 tggttaccat atgaactata tccatgagca gcttctgtag ttatttttct ttgcataagt
     2881 tttccagata aagtatcttc taaagtcgtt cttgggtcag cacttcctgt aacacgcatt
     2941 gcttgataaa cataacttct tcctgataat gggtctctta tagctccacc taaacaagta
     3001 gctgctccac caaatggttc tatttcagtt ggatggttgt gagtttcatt tttaaacatt
     3061 actagatatt tttccatctt accatcaact tcaacatcta catttatact acatgcattt
     3121 atttcttcac tttcatctaa atcatttaat tttccatttt tctttaactc ttttactgca
     3181 acagtagcta tatccattaa acatatatct ttatgtctgt tcacatatac attgtctctt
     3241 gagttaagat acatttgata cgcttctttt actatatcat taaacttacc atcttctatc
     3301 tttacatttt ctatttcagt cataaaagta gtatgtctac aatgatcaga ccaataagta
     3361 tctaatactt ttatttctgt tatagttgga tttctctttt ctgtatcttt aaagtatttt
     3421 tgaacgtgtt gcaagtctcc taaagtcatt gctaatcctt tttcacttac aaatgttctt
     3481 agtccatctt catctaaatc tataaatcca tctaatactt caactgttgt tggtatttca
     3541 gtttccattt ttaatgtttc tggtttttct aaagatgctt ctctactatc tactggattt
     3601 atacagtaat cttttatttt agaaaactct tcatcagtta ttttgcctga taatatataa
     3661 acttttgctg tattaatagc aggtcttatc ccttgattta caatttgtac acattgagct
     3721 gcccaatcac ctctttggtc gtattgacca ggaagaaatt ctattgcaaa tactctttca
     3781 tcatttaatt ttggtatttc ttcataatat actacatcta aatttggttc agagaatatt
     3841 gtctttgctg cattttcata aacttcttct gatattcctt ctacatcata tctatttaat
     3901 attcttaaat tttctatatt atctatgtgt aaactttcca ccaagtcttt ttttaaagct
     3961 ttagcctcaa ggtcaaaacc ttctcttttt tcaactaata ctcttctaac cattgagtct
     4021 ttattttcag tattcaacat accttatcct ccattttaga aaaattatct tcctatgtct
     4081 gttctatagt gcattccttc aaagtttatc ttctttatgt tttcataaac tttttctgct
     4141 gcatccttaa ctgtaactga ctttgcagtt ataccaatta ctcttcctcc attggtaact
     4201 aaattaccat caagcatttt agtgccacta tggaatacta ctatatcatc atccaagttt
     4261 tcaagaccag ttataatttt ccctttttca taactgtctg gataccctcc tgatgtgagc
     4321 ataacacata cagcttcttc atcactataa tttatttcta tttcttttaa cttattatct
     4381 agtatagctt ccattatctc atgtaaatct gtttctaatc taaataaaac agattgtgtc
     4441 tcagggtctc caaatcttac attatattca agtacttttt ctccatcttc agttatcata
     4501 agaccaacaa ataaaatacc tttaaaatta agtccatctt ttttgaagcc ttctaaagtt
     4561 ttttctaata ctgtctcttt tactttattt gctaattctt ctgtatatat ttcactaggt
     4621 gaaaaagttc ccattccccc agtgtttgga ccttgttcat agttgtttac tttcttatgg
     4681 tcttttgcac ttgccatagg aactattgta tcattatcta caaatgctag aattgaagtt
     4741 tctattcctt ttaaaaactc ttcaattact attttatctc cagcagctcc aaacttctta
     4801 tctgacatca tttcttttaa agttgctata gcatcttctc tattttcagg tattacaaca
     4861 ccttttccag cagctaatcc gtccgctttt attactactg gatatccaaa actatctatt
     4921 tctgatatag cttcttctaa gtttgtatat tctttatatt ttgcagtagg tatgttatgt
     4981 tttatcataa attcttttga aaaagcttta cttccttcaa gttgagcaca ttttttatta
     5041 ggtccaaata ctcttaatcc ttctttttca aactcatcta ctattcccat aactagtggt
     5101 acttctggtc ctactattgt cagacctatt tcattttctt tggcaaattt taaaagttct
     5161 tctatattag tatcacctat atttacacat tctgctacat ttgcaattcc tgcattccct
     5221 ggtgcacaat aaattttttc tacatttttt tctttgctta gtttccagca tatagcatgc
     5281 tctcttccgc caccgcctac aaccaatatc ttcataatat cctccttagt gtttgaagtg
     5341 tcttattcct gtaaatacca taaccatatc atacttgtca caagcttcta ttgaatcttt
     5401 atcttttata gagccacctg gttgaactac tgctgatatt ccatattcat gagctaatgt
     5461 tacacaatca tcaaatggga aaaatgcatc tgacgctaaa acagctcctg taaaatcttt
     5521 atctttgtta ttttcaagag cattttttaa tgcccaaatt cttgatgttt gtccacaacc
     5581 taaagctaga gtttgtccat tttttactat tgcaattgca tttgatttca tattttttac
     5641 tactctcata ccaaattcca tgtccttaag ttgtgcatct gttggttttg cctttgttac
     5701 attttcactt ttttctgcta atatgttatt tctatcttgt atcaataatt ttccatctaa
     5761 atatttcatt tcatatggtt gtaaactatt ttctatttta gctaatttta atattcttag
     5821 atttttcttt tgtttaagta tctctaaagc ttctggttca aaatcatatg ctactactat
     5881 ttctaagaat atttcattta atttctctgc tgtagcttta tctatagtag aagttattcc
     5941 tactattcct ccaaatattg aaaccttatc tgcttcataa cattttgtat atgcttcaaa
     6001 agcatctttt cctagaccta ccccacatga attagcatgt tttatagcta ctgaaactac
     6061 ttcttcacta tctttaaatt ctctcattac ttctaaacat ccatgtaaat catttaagtt
     6121 gttaaaagaa agttctttac cgcctaattg ctcatagttt aagattggat tttttgcatt
     6181 tgactgtgaa tataagaaac cattttgatg tggattttct ccatatctta aagtttgttc
     6241 tttttggaaa gttaaattta atatgtcagg atacgtatct ccaacttctc cagcaaaata
     6301 actagatatt aacgcatcat atcttcctgt agttgagaaa gctttatatg ataatttttt
     6361 tctgtcttct aatgtcattg tatctgtttt taatttttct attatagaat cataatcttt
     6421 tatatctact actactatta catctttata gtttttggca gcagaacgta tcatagatgg
     6481 tccaccaata tctatatttt caattatttc atcatgactt tttccagctt ttaaagttcc
     6541 ttcaaaatca tatagattta cagctactaa atctattgaa tgtattttat gctcatttac
     6601 agtttctaca tgagattctt tatctctttt ataaagtata ccaccatgta tatatggatt
     6661 taatgttttt actcttccat ctaatatttc tggaaaattt gtaacatctt caacttgcat
     6721 tacattaaca ccattttctt ttaatgtttt aaatgtattt cctgtagata taatttcata
     6781 atctaactta tttaactctt tagcaaattc cactacacca gtcttgtcag ttacacttat
     6841 taaagctctc ttactcattt ataaacactc tcctaccttg aagttttaat ttattttcac
     6901 aaaaaagact tatactttct tttaaaatcc tatgttcaac ttctaacact ctttttgcaa
     6961 gtgtctttgc atcatcatct tgatttacct taactacatc ttgcattatg attggcccag
     7021 tatcagcacc ttcatcaaca aaatgaactg tagcaccagt tactttagct ccgtaatcta
     7081 taacaccttg atgaactttt tctccataga atccagctcc acaaaatgaa ggaattaatg
     7141 atggatgtat gtttatcatt ttgtttctaa actcattaac cagctttggg ctgattattt
     7201 ttaaataacc agccaaaact actaaatcta ttttatttcc cttaagaatt tctattatct
     7261 tgtcttcatc agtttcacaa attgctttta tgttatgatt tttagctctc tctagcccat
     7321 aagcaccttg cttacttgag ataactactt taacctgacc ttttatttct ccagactcag
     7381 tcccatctat aactgcttgt aaatttgttc ctccaccaga tattaaaact cctatattta
     7441 gcataattct acaccttcat gactatctac tacttcacca attatatacg ctttatcttc
     7501 taataattca ctatattttt tatctacata agcagcattg tcattttctc tattatttat
     7561 aaagtttaca acatcatcag cattttcttt atctactatt aaaactagcc caatacccat
     7621 gttaaaactc ttatgaagtt ctctctcatc aacagcatta aaaccttcta tcattttaaa
     7681 tataggaggc ttctcccaag attttttatt gatatctagt cctaaccctt taggtataac
     7741 tcttgttata ttttctataa gccctccacc agttatgtga gctatagctt ttatgtcatg
     7801 tttagctaat acatctaaag ctaatttaac atatatttta gttggtgtta aaagtgcttc
     7861 tccaactgtc attcctagtt cttcaacata ttgttccatt ttataattgt aagtttctaa
     7921 aaatatcttt cttataaaag aaaatccatt gctgtgtact ccacttgaag atataccaac
     7981 caatacatct cctgatttaa cgttgtttcc agaaacaatc ttttctttgt ctgctattcc
     8041 tacagaaaat cctgctaaat catagtcatc ttctccgtac attccaggca tttcagcagt
     8101 ttctccacca attagtgcac aaccagacat tttacaacca tctgcaattc caccaactat
     8161 tttttcgata tgctcaggaa ctaatttccc aagagctata tagtcaagga agaataatgg
     8221 ttttgctcct tgacaaatta aatcattcac acacattgca actaaatcta ttccaacagt
     8281 atcatgcttg tccatcatct gagctatttt taacttagtc ccaacaccat ctgtagatgc
     8341 aagcaaaact ggttctttca tacctacaaa atcttttaaa ctgtataatc cactgaagtt
     8401 acctaaatct ccaactacat ttccatcata agttcctttt attttacctt ttataaggtc
     8461 tactgctcta tttccttcat ctatatctac acctgattct ttatatgtta acatgaaagt
     8521 agttcctcct tatcaatttt cttaactggg tattctccat caaaacaagc tttacagaaa
     8581 gtagacttat gttctgttgc atctaacatt ccttctatat ctaggaattt taaactatca
     8641 catccaatat attctctcat ttcttctacg ttgttacttg atgcaattaa tttagagcga
     8701 tttggagtat ctattccata gtaacaagag taagctactg gaggagaagt tattctaaga
     8761 tgaatttctt ttgctcctgc ttctcttaaa gattttacta attgtttaga tgtagttcct
     8821 cttacaattg aatcgtctac taaaattata gactttcctt ttattattgt tgaaagagga
     8881 tttaatttta tttttactgc tatttctctt tcttcttgtg taggttttat aaatgttctt
     8941 cctacatatc tatttttaac taagccttca cttattttta atccactagc atttgcataa
     9001 ccaatagctc ctggccaacc tgaatctgga actggaacta ccatatctgc ctttacagtt
     9061 tcattctgag ctaatctctc accacattta attctaaatt catatgcatt tacattgtct
     9121 atagttgcat catttcttgc aaagtatatg tgttcaaata tacaactttt cttaactggt
     9181 ttataatttt ctgaataaaa atatgatttt agttctccat ctttaactac tattatctct
     9241 cctggctcta catctcttat aacttctccg ccaagtatat ctattgcaca attttctgat
     9301 gcaaatatat attcatcacc ctttttacca agtacaagag gtctgaatcc atgtgggtct
     9361 ctaactgcaa ccaattcttc ttgactcatt attacaagag catatgcacc ttttatataa
     9421 tccattgtca cttttaaact ttctactata tcccctttgt aatatctcgc aagtatatat
     9481 aaaatcactt ctgtatctga attagtttgg aacatatatc catcttcttc tagcatatct
     9541 tttaaataat ttgcattaac tagatttcca ttatgagcta tggctaattg tctttttcta
     9601 caacttccaa ctaaaggttg acagttagaa acatgacttc ctccagctgt tgaatatcta
     9661 acatgtccaa tccctatatt tccttttaac ttagataatt cattttcctt aaaaacatct
     9721 ccaactaatc ccatatcttt tttgtactta atttctccat catctaaaag agctaaacca
     9781 cagctttctt gccccctgtg ttgcatagaa tataaagaat aatatagttc tttagttaca
     9841 tctttattag agtaaatccc taaaactcca cacatacatt tgcctcctat ttattattca
     9901 ttcttgaaag tacttccgta taaccttcta caaggtctcc aaggtctttt ctaaatctgt
     9961 ccttatctaa cttttcattt gtatttacat cccaaagtct acaagtgtct ggtgatactt
    10021 catctgccag tattatattt ccttcagaat ccttaccaaa ttctatttta aagtcaacta
    10081 actttaggtt taattttaag aaaaatgctt ttagtaattc gtttattttt aaagtttctt
    10141 ctcttaagaa ttttaattct tctctagttg ctaacttcat tgccactgca taatcatcat
    10201 ttagcattgg gtcaccataa gcatcatttt tgtagcttat ttcaaaaatt ggctcatcaa
    10261 aaactacacc ttcttcaaga ccaactcttt tacagataga accagcagca atatttctta
    10321 ctattacttc taatggtaaa atctctactt ttttaactaa catttctcta tctgataaac
    10381 tttttataaa gtgagtattt atattatttt cttttaacat ttcaaagatt attgttgata
    10441 tcttattatt tagtatacct tttgaagata tttctgcttt tttttctcca ttaaatgctg
    10501 ttgcatcatc tttataataa acaacatatt cattttcatt atctgtagaa tatacttgtt
    10561 ttgcttttcc ttcatataat aacatttctg ctaccccctt aaattcttgt cgtcttctaa
    10621 caccttttgt gccatctctt ctctatatga tttcaaatcc tcttttaatt ttgggtattt
    10681 taaagtcatt atttgtaatg ccaataaagc tgcgtttaaa cctaagtcta ttgtaactgt
    10741 tgcaactggg attccttttg gcatttgaac tattgataaa agtgagtcta gtccatccat
    10801 agttgatgat ttaataggta atcctattac aggaattaat gtttgtgaag ctattacacc
    10861 tggtaagtgt gctgcttttc ctgctgctgc tattattaca tcagtatcat cttctatttc
    10921 ttttaggaat attgaaagtt gttctggtgt tctatgagct gataatgctc ttgctacaac
    10981 ttctattcca tatttttcaa gcagttttat cccttcttct aattttggat aatctgattt
    11041 tgaacccata actactgcta ctttcattag acttcccctc tccgtttata agttatttta
    11101 cactctaaat cattcgtatt attatacgaa ttatacacct taaattttat attaatatac
    11161 atatattatc aactcttttt tgaaattttt taaaaaaaat atttcttgta tttttttcat
    11221 tcgctacgta tcgaacttta tttatgtttt tttgttaaaa taaaaaacag actatctcaa
    11281 aattgatttt ctaattaaat tttaagctca ataccctttt taaaatcttg tatattattt
    11341 tttattactt cttttttaat taataccttg gatttataat cttgtttttt agttctgaat
    11401 gttagcttta cttagataaa accaatacaa agtttattct ttatataagt aatagctaaa
    11461 atttaaagta atgatattat ataaatagaa ataattttat ttaaaggaga atctgtatgt
    11521 tagaagttat aaaaaataga cgttcaataa gaacatataa agataaaaat atagaagaag
    11581 agaaaataac tgaaatacta aagtcagcta tgcaagcacc ttcatcaaaa aacgctcagc
    11641 cttgggaatt tattatagta gatgataagg aactactgaa agagctctca aagtcacagc
    11701 atagagcaaa gcatgtagag tttgcaccat tatgtatagt agtgcttgga aatagagata
    11761 aattcttaaa acctggtaag tggatacaag atttaggtgc ttgcacacaa aatttattat
    11821 tagaagttac taatcaaggt ctagctgctt gttgggcagg agtatttcct aaaaataaag
    11881 tagtaaataa agttagacaa actttagact taccagtaaa attagttcct tatgcactta
    11941 tatctattgg atatagtgaa gaaaaaaatg aatttataga ccgatttgat aaaaataaaa
    12001 tacatagaaa tgtatataaa aatagataat atttagtaat ataaacaaaa atacatagta
    12061 aaaatgcctt tagaatccat ttgtattcta aaggcattaa ctttgactaa gcacctaatt
    12121 agactctaaa gtaaaattta actatcagtt attatttata taccaattga atattatatc
    12181 cttcaatatt tttatttatt tcaaatatat taataatatc ctacaattaa agttatccat
    12241 tttttcatta aagtatttta gtaaattaat aataataatc acaactgcaa gaaaatatac
    12301 ctcttaatga ttattgtcaa aagtaatttt ttgaaaaaac tcatattgac tcttgaagaa
    12361 gtacatacaa ataaatttta tttaatggat atttaaataa tttatcttat aagtatttaa
    12421 tgataactct cctacaaaat tttattagta tataagctaa ctaagctttg atataaagag
    12481 aatataaaat actgtaaaaa agtgttatgg ttatttcgtt gaaaagaaag acatactatt
    12541 aaagcataaa ttacaatata ggagatgatt aaatgagaat actttcagaa actgaaaaaa
    12601 tatctttagc agcagttata aaaatggaat ctgatggatt acttatgcaa agagcaataa
    12661 atatgttaat atcagatgaa gacctaaaaa gacaatcaga atctagtata ttagcaacag
    12721 aaggaagaat taaagctata caacaattta tagttgaaaa tgaaatacta atttctgagg
    12781 aggtatagat aacatgagaa atatgataga aaatttaatt aagaacaata cttatataga
    12841 cgataaagta ataatgttaa gtatgctttc atctgctaaa gcatctgcca atatgtatct
    12901 taattctgca ttaacaagta gtacacctga actaagagct atatattcag cttcactaac
    12961 tcaaatggta gaaggtcaca cagcacttac agaattaagt attaataaag gatgggttaa
    13021 accatatgat aaacctattc aacagttaac atgtagttat aaagaatctc aaaatgttat
    13081 tgattaatct aaataaaata tatttctaac tatttcattt taattaaata ataaaaaggg
    13141 gatattcaaa tttttttaag atttcccctt cttctaaata atctattttt aacataaaat
    13201 agattgctca caatctaaat atataatttt atatagttat tattaactat tttatacaat
    13261 tctattatta acattttttt actacatatt tttataacta catattttta taaataaaga
    13321 catcaaatcc ttatattcca aaattacaaa atccggctta taaaacaaat cttctacctc
    13381 atgtccccta tggttcaacc atatactctt ccaaccagct tttttagcac ctaatacatc
    13441 atttctataa gaatcaccca cataatatgt attttctcta tctaaattca taacattctc
    13501 agctactcta aaaatgttag catctggctt agaaaatcca acctcactag agataaaaat
    13561 attactttta tcaacccagt tttcaatatt taactgcttt aacttcattc tttgatgggt
    13621 tgatggacca ttagttataa ttcctagatt aatatttctt tctttagaaa aatttaaaac
    13681 ccactcaact tctggtatta gtgtaatttg tgattgttga tatatatact catcttgaaa
    13741 actttgtgca tctttttctg taatagaatt tcccaactcc tcaaaagctt tcattattct
    13801 atagatatgc atctctttaa ttggcatttc tccattctca gtcatatgaa atacctcatc
    13861 actatacttt ctactactca tatataaatc ttcaatagaa atatccttta ttgaagaaaa
    13921 cactttatta tatgcagtgt aaaaaggcgt taattgatta tataaagtat catctacatc
    13981 aaaaattaaa ttaatcaaaa tattagtcct cctaaattta atagtggtaa gtataattat
    14041 aactataaat acctagtata acaatactca ccactataag tcctattatt tatttaaaat
    14101 cctcgcagta acacctttta agttatagca ctctgcataa tcacgcaaag gacgtttaat
    14161 atggtctaca acaaatcttt gaccatcaat attattctta cttaaattag cataaagtac
    14221 ttctatttct tttttaacat catcattatt aaatgtataa tgtcctgcaa tatctaatat
    14281 ttcattagat aaaacttcat tcttaaatat ctcttcagta gtaagatttt tttgttctcc
    14341 tattacccat tttctccaac gtccacattc aattgcatta acaagaagaa catgtctgat
    14401 attagaagtt ttatcaacta gaccctcttt ttctaattta cactcaactt ctgctaattt
    14461 taaatatgcc ctagtttctt cagtaccata ttgaggagct acatttgtag caataatttg
    14521 agatggtata tgttctaata aagttacatc atctaagtaa tcaccattat gttcttttaa
    14581 accaactcca tatttcttag ccatttgagc taaatcataa gcatttctaa aattaaaagt
    14641 tcctacttgt tctgtctttc tagttaaagt accagtttga ccaactataa atgttggcat
    14701 tggcaaacct ctcttatcaa gttcaacctt tagtctagtt ataaatgttt catatgtctc
    14761 aattgaagtc agacctccat tggtttcctc tgtaccaacc tcataaccta tgtctggtag
    14821 attaagtctt tttctctcgt tttcacaata ctcaattaat tctacagttc tttctagtac
    14881 tacatctagt ggaattattt ttccaatttc aaatgggtct tttgtagggt caatcatcaa
    14941 taggtcaaaa cctgcttcaa tgtccgctaa ataagatttt tttcctaact ccattgcttc
    15001 atcaacaggc aaatggtcat ttctctcttt atctctttgc catggtccac catggtctct
    15061 acataaataa tataaaccat caaatccaac tttgtcagca atcttcttaa tatcttttgt
    15121 aaaagtttcc tgattccaac catttacata accgccacct agttcatctg catctacttg
    15181 atttctactt gcgataaaca ttaaaggaaa atcatcatct tttgctagtt caaaacttgc
    15241 ttgtaacaag tttggtgaca taggaccaat tcctaaaaga gttgcacttt tcccctcttc
    15301 ttgtaagtct agtaatcttt gtacgacagt ctttattggt aactttttca tgactatatc
    15361 tccttttatt ctatttttaa gatattttca aagatgcttt taataaaaat ttctttaaaa
    15421 acatctttga gttatatgta aaataaatca atttttattg aaaagtatct tttattttct
    15481 taattaatgc atcagatttc ttaattatgt cagatacact cattcttact accttctttc
    15541 ccttaaaacg ttccatattt ctaatctcaa catcattagt tagtattaca caagttgctt
    15601 catcaatatc tttaagtgac aattcattat ctattccaga gcttccttga gtctcaacct
    15661 ttatttctat accaaatttt ttggctgata gttcaagtga ttcagctgac atgtaagtat
    15721 gtgctactcc actaggacaa gatgttactg ctacaatttt catatactta tctcctttaa
    15781 atttatagtt ttatatttac ttttttatac aaattccttc catttatatt tataattctt
    15841 caaatattaa ttcaattgag tcatctaaat cctcttcaat ttcttcatct tctttcacgt
    15901 ctttttttac taatttcatt aatactgcaa caactactgc tccagttaat actgatgcaa
    15961 tataccccaa cctattgtca actacaggta atactatcag acctccccag ggtgcatgat
    16021 ttaaacatcc taataaaaaa gccataatgt ttcctataat cgacccagcc ataatacaag
    16081 gtattactct aactgggtct gatgttgcaa atggaatagc tccttctgta attccaacac
    16141 atcccattaa tagcgaagct ttacctgaat ctctttcttc cttgctaaat ttttttggaa
    16201 acaataatgt ggctaatcct aaaccaattg gaggtataca aattgcaact ccaactccac
    16261 ccattaaata tggaagtgtg tcaacttgag tttgggcaaa agtagcagca accttattta
    16321 taggaccacc catatctgag gctatcattc ctcctaaaat tccacctaaa ggaattttag
    16381 aaacaccaga cattccattt aaccagtttg tcatccatgt cataaaagaa gctataggtt
    16441 ctccaattaa aaatacaatt ataccacctg taatcaatgt accaatcaaa ggataaataa
    16501 atatagaacc taaagttctc attgtaggag ctaatttaat tttctttaat tgtaaaacaa
    16561 caaatcctgc tataaatcct gctatgatac cacctataaa acctgcattt acttcttttg
    16621 ctaaatatgc acctatcata cctggagcta agcctggttt atctgccata ctaaatgcta
    16681 tatatccacc taatatagga atgaataaag ccaatccagc agctccaatt tgattcagtt
    16741 tagccaacca tcctgtttca ggaacagcag cttgtccact tagagttact gataaagcaa
    16801 ataatattcc tcctgcaacc acaaatggaa tcatataaga tgttgctgtc ataagatgtt
    16861 tttgcaaaaa ctttaaaatt ctcattttaa tcttcctcct ctattttttc ttttataaga
    16921 ttataaacag tttgttcatc tgaacatcta atttgatttc taaaactttc atgcatcaat
    16981 aaacgtgcta attttgataa aatctttaaa tgaagattat tttcattgtc ttttgttact
    17041 gatattaaaa atattaaatc aactttttct ccttcttctg tccattcaat aggagtatca
    17101 agctttccta ttgtaataca agagttttta acacctacac tttgactatg aggaagacct
    17161 ataccatgtc caatataagt cgacaatgtt tcttctctct ttaaaacatc ttctaaaaat
    17221 tgatttttat ctattaaata tccttctttt gacactacat caactaatga tataatggct
    17281 tcttcttttg aattaacatg tattttaaat gaacatttta cattaactaa tttactcata
    17341 cagtgacctc cttctcatat ctatgaatta aaacttctgc ttcttctttt gtctttgcac
    17401 tttctaacat agcaataaaa ttagtcttat aaaccatttt agttagattg attattgcag
    17461 gaatttgctc ttttttatct ctgcttgcta gacaaaatat gattttaacc tttttatctc
    17521 taaaactaac tgcttcttta catattaata aacttatcga acttactttg actaatgaac
    17581 tatcattgcc atgtagtaaa gcaaattcgt tatcttttac agcataaaaa cccacatttt
    17641 ttaaaatatc tataatattt tcagcatatt gtgctgatac aaaatcacaa ttagcaagta
    17701 tatttgaact aatttttacg gcatcttccc atttcatctc ttcatcaatt atccttatgg
    17761 tatcaatccc tattaaatca cttaaattaa gttgtctaaa taatattctc tcatcactgt
    17821 atccaagttc ttgacatata atactcaaca ctcttttttg ggcatcttct tctaaaaaat
    17881 ccaacctttt ttttattgac atataattag ttaaaacgtt ctttcttttt atcccttttt
    17941 cttcaaactt caaataatcc tcttcttgta aaaatggatt tatctcaata attgttttag
    18001 gcaagtctat tactatcttt gatgttgtta taatgaagtc aatactttcc cagtctttat
    18061 aatcttgtaa cttatatgat ggaatactct ctactatatt tatctggtaa tcatttacca
    18121 aactatcctt taacatagca attacaccat atccaaatcc acaaactaac aatatatttt
    18181 tgtagttatt cctctcattt ctatgtatac ttcctaagaa atgtattgcc aaaagtattg
    18241 tttcattttc attctttatt tgacttaaaa ctctgtttct ttgaatagaa accttagtag
    18301 cttcaagtac atattgatat ttatctggta ttacatggag taattcttca taaatctgta
    18361 tattctgttt gattctctct atcaatgggg caatatgatt gagtaatcct ttataaagaa
    18421 tcatatcttc tttaaagcta atctctaata tttctgacat attgtaaatt aaatcttgaa
    18481 ctataatttc tgtactaatc aaatccatat cttcgtttaa acttgcatat ttatttgtaa
    18541 aaaatacaaa actctttaac agaaaaattt gttccttagt caactttttt tgaataattg
    18601 tttcaaattc ttggtcaaac ttattttcaa aacacggctc acttaatgaa gcagattcta
    18661 aaggatgttt ttcatctgaa tgtatatacc atgtaaaaag gaaaatgttt gacacatacc
    18721 atgtaaaaga ttcatcattt aaagtccatc ccatttgcaa taataaattc atagaccaat
    18781 tatatatgtc aatcaaataa atccctttaa atattttttc aatcacgttc actaaatact
    18841 tctcaaatac acttaaatca gatttttcct taccaaaaag ataagaatat gattttaata
    18901 tgttcaatct ttgcttatat ataatgtctt cttctccact tattttaaaa gctttatcat
    18961 aagtaatagt tatttggttt ctttctaatt cacttgacaa aacattaagg tcatttttaa
    19021 ttgaacttct agaaacctga aattctttac tcaacttttc taagtttaga ttatctattt
    19081 taaacattga tataattttc ataatagata ctcgctcagt taaggaaaat acatattcgt
    19141 tattatttat acatactact tggaagtcat caggaataat taacacacct ttgttcattt
    19201 tttgtattac tgacttgttt aatgaacgca aaacaatatt taaattattt atatcatatc
    19261 taacttttct ttcttctata ctaagttgtt tgctaatatc cttgtaactt gtcttatttt
    19321 ttccttttag aaaattcatg atgagtaaca ttctcccact tatcatattt tccctccttc
    19381 caaaataatt agagttatta atatagcaaa ttttatatta ttatgtatgt tttagttttt
    19441 ttatctaaga cagtaactat ctttctatat tcagaataat acaataaagc tttagccaca
    19501 acttacaaca tttgaaaatt atttttacaa aatctgcaaa tacattttta ttaaatatat
    19561 aaataaaaaa gacaagttat atttttaatt atataacttg tctaaatttt ttatcttata
    19621 catttaataa tagatatttt taattttatt attttcatat gcaaaatttt caaagtcaga
    19681 aattaataat ggtttagaaa aaacataacc ttgaatataa tcgcatccca cattttttag
    19741 gaactcaact tgttctaatt cttcaatgcc ttcagcagtt actttcatac ctagcctttt
    19801 gctaagctca ataatagact taataactac cttaccacgg tctatattat ttttgcttac
    19861 aaagaatgaa cggtctaact taattatatc aactttaaaa tcctttaata ttcctaatga
    19921 tgagtatcca aatccaaagt cgtctataga acatttaaat ccattagaat gaatatcatc
    19981 tattacatct acagcttctg gattttctaa gaaaatagat tcggtaagtt ctaaatctat
    20041 catatatgtt ggtacattgt atttattgca tataagctta tatttttcta aaaacaattt
    20101 atcattcaaa tgttggcgtg atacattaac agaaattctc aacacttctt taccttcttt
    20161 tatccactta gaaatcaatt tgcatgtttc ttctagtata tataaatcta acttacatat
    20221 aaatccactt ttttcaaaaa gaggaataaa atcacttgga taaattatgc ccttttctga
    20281 atttttccaa cgcacaagag actctgctcc actaattttc ccacttttta tatcaatttt
    20341 aggttgtaag cacataaaaa attctttatt ttcaagagct ttttccatta aatttgaaat
    20401 ttccttttca gatattagtc gttttctttc tatatcacta taaaaagaaa aatcactttg
    20461 atgagtttta ttaaagcttt ttcttgctgt atttgcatgg tcttgtattg taattaaatc
    20521 ttcattatga ttttcaattg tgtaaatacc tactgatatt cttaaaaagt atggattttc
    20581 tctattttta ttaaaataat ttgtatcagc ttctatttta tataataact ccagggcttc
    20641 taacactggt ctattcttaa taagcaaata aaaaaggtct gcatcatgac ggcacacaaa
    20701 ttcttcattt tcatcaatat ttttatacaa aacattgtaa agatgtttta gtgtcatatt
    20761 tcccttttca ctaccaaaac aatcatttat tagcttaaaa tcttttatat ttattgctac
    20821 catcatataa gtactatcag gtgattctga aattaaaggt ttgactagca tactaaactt
    20881 agttgaattt ataccatttg ttatactatc taaatatgca atattttcta tttgatttac
    20941 atattttctt cttataatat ataaaattaa tgttataatt ccaataatac atgtaattat
    21001 aaatgtcatt attattgtaa aagaatttaa cttattaagt tcaccaagta cagcatcttc
    21061 aggtactatg agaagaaaat gccaatcatt taaacctctt gtaattgaat gataagttaa
    21121 taagcattgt acaccttttg atgaggttat tgtaatattc cctgatttat catttttaaa
    21181 atcattaagc atcttttgtg cccaaggttc atctccagaa cccttatatt tatggctaag
    21241 gtcttttatt aattcagtat tttttcctat gaaaagaggt tttcccttac tattaataat
    21301 ttcaatagtt ccttctccat taaagttatc tatattaaga atctcattca tactttcttt
    21361 atcagataca cctattaaaa tactagatac ttcatgatta tcattaaata taggtacact
    21421 ataaagaaca aaacccattg actctattat ttctgagtat tctttacctt tgattccttt
    21481 tttataggaa tcagtattct ttaagtcaaa cttttttcca cttaaaaact cagctttacc
    21541 ttctaaatct attattccaa tatcataata cttataacta gtctttcttt tagataaata
    21601 ctcatatcga ctatcacctt catattgctt aataaaatct gccattattc ttaagtcgga
    21661 taaaataaat ttgattttag aatcaacatt cattacaatc tgtgtagata catcatttaa
    21721 atagttttta gattgtttat tagatatatt gtgtagtttt acggtattaa aaacagtaat
    21781 tacaaggatt gaaataataa gtgaacataa aattatagac agaactaaac tatttttctt
    21841 ataccttgat aatttcattt ttttcctcca aattataaac ctatttttta atgtatatta
    21901 acacttacaa atttttttac taagaatcct tcacactata ttatagtatt ctatttctta
    21961 atattcaaga atgccatctt aacatttcat taacattcca tagataatgg ttttattaaa
    22021 caattatatt atatgcaatt tgaaactata tttgtattac aatttatcat tgtatcattt
    22081 tttatttatt tttaaatttt ggtcaaaaaa tctaaattgc ccttgtatat gacaccatgt
    22141 cagggtatat catccattta gagaggtgaa atataaatga aaataaataa ctataaaaat
    22201 caaagcatca tcacaaatcc taaaaaattt gaaaataaat atcaagattt accaaagact
    22261 ccagttgagt tattaaaagt ggttcaaagc ctagtaattc atggagacca aggaaaatta
    22321 tatggtatat cctttaataa gcaacaatct gatgaggaat tgcttcgtac agttcctcaa
    22381 atgttaaaaa gaatatttga aataaattct aacccactaa ctattccaag aaacccaaaa
    22441 caaaggcttg ttggaatgtg cagagattat tctttacttc ttgtatcact cttaagatat
    22501 cgaggttttg aggctagaat gagagcaggt tttgctaatt attttgaaag tgagcttact
    22561 tatgaagacc actggcttgt agaatactat gatactctaa caaagagatg gataaaaata
    22621 gatgcacaaa tagatgacat acaaaaaaat tattttcaaa ttaattttga tactcatgat
    22681 gtcggaaaaa cagatggttt tttaactggt tcagaagcct ggataagatg tcgacaagga
    22741 catgcacatc cagatgactt tggatataat aaaaattgga aaggttggcc ttctgtaaag
    22801 ggtaatcttc tacatgactt taataatatg attggtttgg aattgcttcc atgggattta
    22861 tggacggaat taagttccaa aaaatacaat caattaacaa gagcagaaaa aaatttatta
    22921 gatgaaatgg cagagatatt atcaagcgga aatataaaaa tagaagacct taatttgtta
    22981 atagaaaaat tgcctgaaga ttatttgaaa tcaatctttt ctcagttaaa aatattaggt
    23041 atttcggaaa taaaagaatt aggcaatcct cttgaactag aaaagaaatt caaatttaca
    23101 aaagcaataa ataaatcaat taaaaattca ttttgtcata ataaaaattc tatttattta
    23161 aagggaggaa gacaaaacaa tttaaaagat attgaggtta caattccaaa gaataaaata
    23221 actgtgatta caggtgtaag tggtagtgga aaatcttctt tagctttcga cacgatttac
    23281 gaagaaggaa aaagatgtta ttttgaaaat ctaggtaatg gagcaaaact ttcagaacag
    23341 cttcaaaaac cggaatttga tttacttcag ggattaaccc ctactatagc tatcgaacag
    23401 aaaaaaggaa gccaaaatcc tcgttctact gttggtactc ttacaagcat ttgggattac
    23461 ttaagaatgt tatttgtatc aatcggaaaa agttactgtc catattgcaa aactccatta
    23521 gaaaaaaaga ataatacaaa aaattattgt ccacattgcc aaactatttt ttctaagata
    23581 aatacttcaa ccttcaatgc taactcccat actggagctt gtcacgattg taatggattg
    23641 ggttttacct atcaagtcaa tcctcaatta attgtaaaag acccaactat ttcaatatta
    23701 gatggagcta cttactattt tggaaaattg agagggaaaa agccaaatgg taattggatg
    23761 gtaggcgaat tatatgcaat agcaaaagat aaaaatatag acctagatat accttggaat
    23821 gaattgccaa gagattttat agatgcaatc ttatatggta cagacgataa gatatatgaa
    23881 tttagttttg aatcaagggg acgagaatca aagattagaa gacctgcttc aggagcaatc
    23941 aaccatattc aaagactttt tagagaatct agttctgaaa ataataccct gcatcaatat
    24001 atgaacaaaa ttccttgcaa tacatgtggt ggcgaattgc tgtgcattga agctaggttc
    24061 actacaatca aaggttatcg cttccctgaa ctaacaaaaa tgacgataga acagttatgg
    24121 aattggttgt gtgaattgcc aaatcaactt caacaaaatg agctatcttt agtaaatgat
    24181 atattgacag aattaaaaat tcgaattagt tatctattaa aggtaggtct tagttattta
    24241 tcaacagata gaacagctcc aaccttatct ggaggagaac tacaaagagt tcgattatca
    24301 agtcagctag gaagtgaatt agtaggatta acctatattt tagatgaacc atctattggt
    24361 ttacatccaa gagaccataa tctaatcatc aaaatgatag aagaattaag agataagggt
    24421 aatactgtta tagtagtgga acatgataag gatacaatac tgtctgctga ttatattatt
    24481 gatgtaggcc caaatgctgg tacaaaagga ggcttcatca ttgcagaagg tacaacccaa
    24541 gaaattataa aaaatccaaa ttctataaca ggcaaatatt tgagtacata caataagata
    24601 atctcacaaa ataaaattat accaagtaaa tggttaagct taaagggatg ccatgcaaat
    24661 aacttaaaaa atattgatgt agaaattccc ttaaactgta tgtgctctat tacaggtgtt
    24721 agtggttctg gtaagagttc tcttgtattt cacagtttac ttcctgctct agaagaaaaa
    24781 ttgaaacaaa aatctatccc aaataaaaat tatactaaac ttactggatt tgatgctatt
    24841 gacgatttta tcttaatgga ccaaacacct attggaaaat ctagccgttc tacacctgct
    24901 acatatatca atatttttga tgaaattcgt tctctatttg ctgaaactcc acaagcaaaa
    24961 caaaaattat tggatgaaag ctattttagc tttaatagca aaaaggggca atgcccaaac
    25021 tgtcaaggat taggtaaaac aaaaattatt ctacaatata tggcagacca atgggtaact
    25081 tgtaatgaat gtcaaggtaa gagatatcaa aaagaaatac tgtctataca atacaaggga
    25141 aaaacaatta cagatatact tgacatggaa gtagcagaag caaaaacatt ttttagtgat
    25201 tgctctgata tatatagaaa actctcctta ttagatgaag tgggacttgg atatctaaag
    25261 cttggacaaa atacattagg attaagcgga ggagagtctc aaaggataaa attagcaaaa
    25321 gaattaggga caaaaactaa aaaacgaatg ctatacatat tggatgaacc tactactgga
    25381 cttcatttta aagatataga aaatttgtta attactttta gaaaacttgt aaatgaacaa
    25441 cattcacttt taattattga acacaataca gagattatac gtgtttctga ttggattatt
    25501 gatataggtc cagacagtgg aataaatgga gggaaaattg ttgcttctgg aactcctgat
    25561 gaaataaaaa taaatccaaa tagcattact ggacatttta tataaaaaca attaaagaca
    25621 catgaattta gatataaaat tatag
//
LOCUS       DAEPXF010000042        24905 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain HB27
            SAMN13376389-rid12479423.denovo.044, whole genome shotgun sequence.
ACCESSION   DAEPXF010000042 DAEPXF010000000
VERSION     DAEPXF010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13376389
            Sequence Read Archive: SRR10518432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 24905)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24905)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001069624.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001069624.1
            Genome Coverage       :: 315x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/15/2021 01:54:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,165
            CDSs (total)                      :: 4,076
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 89
            rRNAs                             :: 10, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 10 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 19 of 69
            Pseudo Genes (incomplete)         :: 44 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 10 of 69
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24905
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13376389-rid12479423.denovo.044"
                     /strain="HB27"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="China: Hebei"
                     /collection_date="2017"
     gene            401..1810
                     /locus_tag="KRL67_001495"
     CDS             401..1810
                     /locus_tag="KRL67_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBOAT family protein"
                     /protein_id="HBH1522384.1"
                     /translation="MIFSSLIFLFLFFPVTLICYYLANPKYKNVMLLLASIAFYAWGE
                     PKYIFLMILSIIVNYIFGIKVSGSKDKKMWVVLSVIYNISILAVFKYSNFFIDNINFL
                     FNTNITIPEVKLPLGISFFTFQIMSYVIDVSRNDAKVQRKLANLALYISLFPQLVAGP
                     IVRYQTVADSIEHREHSWDKFTKGVNRFVVGLGKKVILANQLGIIADGVFNKSAGDLS
                     RLEAWLGIVCYTLQIFYDFSGYSDMAIGLGKMFGFEFLENFNYPYISQTVSEFWRRWH
                     ISLGTWFKDYVYFPLGGSRVSKAKLYRNLFVVWFLTGMWHGASWTFIIWGLYYGILIS
                     VEKAFLEKFLYKMPRIVRHIYLLLIVMIGWVFFRVDDLPAAIKFIGIMFGAGNYPVIS
                     NAFNVFIIEYYYVIVGAIILSTPIVKVVKSLLIKKNNNTLNSTLYQVTNAFFIISCMF
                     IVIVMLSSSTYNPFLYFRF"
     gene            2038..3018
                     /locus_tag="KRL67_001496"
     CDS             2038..3018
                     /locus_tag="KRL67_001496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH1522385.1"
                     /translation="MISINTKTQASLNKTKVGNYYLGKDNWILGMFSEILDQKQLNRY
                     EKSINYLSNLSKDMGKDVYFTMMPHKTNMLKHLYPKYVDNMDNIDINLKNFKNRLNPD
                     LIKYIDMDEYFLSNYSDKERENLYFKTDHHWTGYGAFEGFKMMASNMDLSLSKQDLDK
                     HFSSYKKSVITDKKFIGSYNQNIDMLIKENEYVVYMTKGNQDYSFDINGEEKEEKEVY
                     ATERDNKEWDYGGAYIRGAKTNILTIKNPKALVDKKILIFRDSYQAPTTLLLADMFKE
                     VEIADPRNINSIDKTYKEMIEESNADIVMFMYNSFGFAQMLDAMIDKGIK"
     gene            3218..4201
                     /locus_tag="KRL67_001497"
     CDS             3218..4201
                     /locus_tag="KRL67_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH1522386.1"
                     /translation="MKEVFNAHITQKQNKKIKQKYYLTKDGWLLGKFAKLLPDYHIKS
                     YSKAINNLSNLNSEKDFYFVSLPHKTNMLKHLYPSGIEGKNNIDINKENLEKQLTDIN
                     FLDTDDYFYRSFTEKERENFFLKTDHHGNSLGAFEEYKFILDKLDLGLSKSEIDNHLK
                     RYSTRYVNDKIFIGSYNKKMGYPLKKKESISYVEISDAKYKYFISNGTKNTEINENLI
                     KSKLKDKKEWDYGGAYMRGCDCHILKIRNSSSLTNKKALIFRDSYQAPMTWMLADTFK
                     ELEILDPRYIKNIDMTYDEIIKNSNANVVLFMYNSFGFAGMIQEMIDKGIA"
     gene            complement(4666..5109)
                     /locus_tag="KRL67_001498"
     CDS             complement(4666..5109)
                     /locus_tag="KRL67_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH1522387.1"
                     /translation="MKKNMLYLGIVYFICGILLVLLATFTEFSFEAFIWGLSGAALGP
                     GICMILQYMYWSKPERAVDYEEKIKNQRIEMNDERRVMLRDKAGRITNQIMSYVLVIL
                     IFIVSILSVFSVMVISKWVLVVLGLLILFQFICSVVVYNKLDKKL"
     gene            complement(5112..5309)
                     /locus_tag="KRL67_001499"
     CDS             complement(5112..5309)
                     /locus_tag="KRL67_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004631111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBH1522388.1"
                     /translation="MKNRLEEIRKERGIKQEELASVLQVSRQTIGSLENGRYNPSIIL
                     AFKIARYFNMNIEEIFIYEEE"
     gene            complement(5501..6367)
                     /locus_tag="KRL67_001500"
     CDS             complement(5501..6367)
                     /locus_tag="KRL67_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HBH1522389.1"
                     /translation="MEWINKLNQAITYIEENLESGVDYAEAAKIACCSTFHFQRMFSY
                     IAEVPLSEYIRRRRMTKAAFELQNSNIKILELSEKYGYDSPTSFNRAFQNIHNISPSA
                     ARAKGVVLKAYPKMTLSIYVKGNVEMQYRIVEKKGFRIVGIKESMNMIVEECFEKVPK
                     LWAKCIKNGTIDKLSELINNEPCGLLGVSVCTNSKGLDYYIAAPTDKPVLEDTHEYLI
                     PSGTWAVFECVSPMPNALAIQELQKRIITEWLPSSGYVYANLPDIELYPNGDINSPDY
                     TTEVWIPIQKPS"
     gene            complement(6593..7462)
                     /locus_tag="KRL67_001501"
     CDS             complement(6593..7462)
                     /locus_tag="KRL67_001501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HBH1522390.1"
                     /translation="MDYKTIILNRKSVREYKNTDIKEEYLSEIKKYSESCKKLVPEID
                     VDVRIMNRSEVFNNLDKVAGYKGNMIDSPSYIIILSDIKDNYIENSGYIGESIIFKVT
                     ELGIGSCWVTFEDGKKVKEKLGINSDKEVTGIIAIGYGENASNAKVLNATKTGQNYSK
                     SDMQVVIDKGSTRLGVEDVVYMNEWGNVASIEILEERALLDAFHYARLAPSTLNRQPW
                     RFIVDGGTVVLAVRKDGHTNLYEEKIDIGIVMLYFATIISATMFDLKWNLGTPDKDYK
                     VPEDYKIVGYCNI"
     gene            complement(7967..8188)
                     /locus_tag="KRL67_001502"
     CDS             complement(7967..8188)
                     /locus_tag="KRL67_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH1522391.1"
                     /translation="MNVNNHKCPECGEHSKKFEIVEENEDCVIFECYTCGTISILKYC
                     ENCKEFVFVNIPNNVETYEYCCGKCNNKI"
     gene            complement(8689..9669)
                     /locus_tag="KRL67_001503"
     CDS             complement(8689..9669)
                     /locus_tag="KRL67_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH1522392.1"
                     /translation="MGDTSRFYESVKNKYPKLKDGTRVHLWPKENIIIGPIYDEEDGY
                     KKSIGNVDSLSWKQIVILAECDGKNTTDDIINILSNRYRNIHNMKEKVMEFFMFYENV
                     YLTFEDDIQTLSSVFEITGNKSYITPLYFTIEIDGDNNTNDYFSEITSLLNCMYEKGC
                     RFIEVIGEDILKNKSMREIFQYMLDHFDLIVVTKDSFTIDRSLIKELDNYRHKVIWKV
                     YSKNDDTRIKLKEDIKITSLIKRGHTVVRGDREKAIKIEDMRFKDTKERFSKYGAEWS
                     HLYISSDGSVKSYSFQKDKRIYLGNIFNNSVEEIFLEMQNKIDKLEIVYQ"
     gene            complement(9833..10705)
                     /locus_tag="KRL67_001504"
     CDS             complement(9833..10705)
                     /locus_tag="KRL67_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HBH1522393.1"
                     /translation="MIIKVSMTVLVLILLYILYKYETQHIETTEYTILNKKIPKEFDN
                     FKIVQVSDLHNKVFDKNNKVLIDKIESLNPDVIFITGDLVDGENKNFQVALDLIDNLV
                     EKYEVYHIIGNHEQKSLIKKYKHLYKDYFKELYSKKIINMENESVRIYRGNSHLNLYG
                     LIIPLECYPYLFANNKKLELSDNFVEDNLGKVNENEYNILLAHTPFFFEKYAKWGADL
                     ILAGHVHGGIIRIPFVGGVLSPNREFFPKYDWGKYEKDNSTMILNKGLGGSKVLIRLN
                     CKPEIVKITLKYEK"
     gene            complement(10879..11430)
                     /locus_tag="KRL67_001505"
     CDS             complement(10879..11430)
                     /locus_tag="KRL67_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBH1522394.1"
                     /translation="MDNKFPNVGENLRLLRQEMGISLDKASKMTGVSKAMLGQIERGE
                     SSPTVSTLWKISSGFKINFTTLLNENTNTYEVIKKEEVEPIVEQKGNMKLYPIYPFSP
                     QRRFELFIIELEENCTHVSSTHSHVLEEYVLVIEGKLDLNVDGKTYILESGHSIRFDG
                     TLEHVYKNLNKGKTIFHNIEVYR"
     gene            complement(11450..12352)
                     /locus_tag="KRL67_001506"
     CDS             complement(11450..12352)
                     /locus_tag="KRL67_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl-radical enzyme activating protein"
                     /protein_id="HBH1522395.1"
                     /translation="MSKKGRVVKVQHFSVNDGDGIRTTIFLEGCKLKCKWCSNPDSWS
                     NIVKLGVMKDKCVSCNRCIDVCPQNISSLYDRTQINNKCDLCGECIKVCLKDAICIMT
                     EEMSVEEIVEEVEKDFIFFFESNGGITFSGGEPTLQIDFLRELVDIFYDKGINIAIET
                     CGYFDWNKVNDVFEKIDHIFVDIKSMDDNIHKEYTGVSNKIILDNICRLSKLNKSMVI
                     RVPIIYGVNDSEENIRSTALFVKQNVPGGKMELLPYHKFGIDKYKALGLEDYIYEFDE
                     ICNNHMLKLKEIVELTGVKIIEYK"
     gene            complement(12367..14739)
                     /locus_tag="KRL67_001507"
     CDS             complement(12367..14739)
                     /locus_tag="KRL67_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase/glycerol dehydratase
                     family glycyl radical enzyme"
                     /protein_id="HBH1522396.1"
                     /translation="MSQTNRIEAFRQEYINSKPMICCERARIFTESHKKTEGEAICIR
                     RAKAFLETCKELPIKIFENELIVGTAGKFRRTGILTPEFSWQWVDKEMDTFDKRTQDP
                     YVISKEQIEFIRKEIFPYWKGKSLEEVFLARIPEDTAKILVDTGIIDNDSKWRQAVGE
                     VTPDYQDILFVKGYKGIKEDADKKIKELDISVSENIEKIDFYKSVSIVAQGIMTLAQR
                     YSSLAKEMSKQETDEKRKLELIKISEICMNVPANPPTNFYEAIQFVWFVQLGGILSEN
                     PLALNLGRFDQYMYPYYENDAREGKITESEAQELIEALWIKLSEWVWTISANTANYFA
                     GYNQFQNLTVGGKKRNGTDGTNDISYMCLKATESVKTHQPGLSVRVSQGAPDNFVMAV
                     AKLVKQGTGFPAIHSDSAGAQMLLQDGYDAEDARDWSNCGCVVPHFRKTGQWTSAVNI
                     NFAAALEYAMNEGKSRLTGEKMGLDAKNITEFTSFEELKDEFLKQLAYLVKSSVIGTT
                     VAQQIHKEMVPRPFLSTCVDGCLDKGVDLSKGGAKYNIGPVLTGIGLGVVSNSLAAIK
                     KLVFEDKVTTLEELTKALNNDWEGYEELRKLALDVPKYGNDNDYVDSLAIEVSDFYYT
                     ETRKYKDIFGSKFNSAFMGISNYVPTGKIVGATPCGRKATKPLTEGVSPFVGTDTTSP
                     LAAMKSASKINHDVHTGGTLLNLRLNQDLVETERGLRNLTSMIKSYFALGGFHVQFNT
                     ISNDTLLKAQENPEEYKDLLVRVAGYSTQFVNLSREMQDAIIARNSHSNF"
     gene            complement(14960..15649)
                     /locus_tag="KRL67_001508"
     CDS             complement(14960..15649)
                     /locus_tag="KRL67_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH1522397.1"
                     /translation="MKVKRNINRVAIIVALNLSLALMVGCMSPVSDKNLSSSEQISKG
                     SLKEQVEDKKEESIELFNNYSQKINDAKGREEVKKDLSQRFSKAIIDSGYSITDSKSD
                     GSFMVSINGAEKPPEKNIRSLFYSMSEDDKEGKVTIDILCAKEYPEDDKLSESDKYIK
                     FIYNLFKSLTNTNLTEKEIFSMVEEEFNKGVGVVELPYMKDIHVEVNKVKQSTKVLKL
                     SIVYKFDISNH"
     gene            complement(15890..17269)
                     /locus_tag="KRL67_001509"
     CDS             complement(15890..17269)
                     /locus_tag="KRL67_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HBH1522398.1"
                     /translation="MLNFMTELLVVLSIFIQFSIMNGILIYKKDSKKNEKIVFYIVLF
                     LIYIIVQISRLFNGGNYFSNINYLFYILAFIAWGGYYNYFYKIGYIKYSVFFYLFSVY
                     YKCLDAVIYRILFFAITGNVISSKESLLVPRLESIKIQIYSNLIIILIYLIFSFFRKS
                     IKLISQDKRNYVYLLFALLANTMNMFVNYVLKKLERFGSLHSEGYYFDNFVNPKLVGA
                     SSIFLILLFKEIIKENRLKSQAELIKNKLDMQYAHYLSIQESHMRVKKLYHDINNHIY
                     CIDNLRNNSKEIDEYVNNLKDEIKTFKYIYNTGNMILDIIINEKSEICLKKGIKFTCS
                     INFSKVNFVKPIDVSSIFSNILDNAIEACDKIVDENINKYIRIKGTITKSFFVLKCEN
                     SKLNQLTFNKNILLTNKKDKFVHGIGIQSIKSSLQKYNGELLFENSIDKFILNIYIPL
                     DQDTDSWGD"
     gene            complement(17263..17976)
                     /gene="rgbR"
                     /locus_tag="KRL67_001510"
     CDS             complement(17263..17976)
                     /gene="rgbR"
                     /locus_tag="KRL67_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RgbR"
                     /protein_id="HBH1522399.1"
                     /translation="MIKIAVCEDEKETQLLIEDYLENILKDISIEYEIQKYISGEELL
                     ESNLKDIDILLLDIKMEKLNGMDTARKIREVDNEMEIIFVTSLIDYVQEGYEVRAYRY
                     LLKPIELKELKKHVLACIKDIEINKESHITIKNKSNTYKIYLNEIKYIEVQKKDMLIH
                     TINKNFDIKYSLSKIEKELNPYKFIRCHKSFIVNLRYVENIKPNTVILESGEEVPISR
                     YRYKEVKEKFLKFLGDTIC"
     gene            complement(18227..18778)
                     /locus_tag="KRL67_001511"
                     /pseudo
     CDS             complement(18227..18778)
                     /locus_tag="KRL67_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896054.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2812 domain-containing protein"
     gene            complement(18883..19686)
                     /gene="zupT"
                     /locus_tag="KRL67_001512"
     CDS             complement(18883..19686)
                     /gene="zupT"
                     /locus_tag="KRL67_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZupT"
                     /protein_id="HBH1522400.1"
                     /translation="MNNVIFAFLITLLAGLSTGIGSCIAFFAKKTNTKFLSISLGFSA
                     GVMIYVSMIEIFPKAQDALTKSMGEKLGSWSTVIAFFIGMAIIAVIDKLIPQEENPHE
                     IKKMENIEEKNIKKNKSLLRTGIFTAMAIAIHNFPEGLATFISALDDVTIAIPIAIAI
                     AIHNIPEGISVSVPVYYATGDKKKAFYYSFLSGMSEPLGAIIGYALLRNFLNDITLGV
                     VFAIVGGIMVFISLDELLPSAREYGEHHLSIYGLIAGMGVMAISLLLFK"
     gene            complement(19837..21000)
                     /locus_tag="KRL67_001513"
     CDS             complement(19837..21000)
                     /locus_tag="KRL67_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HBH1522401.1"
                     /translation="MDIKEITKSYKDYVIKLRREFHENPEKSMEEVRTSKRVKEELDK
                     IGIPYVSAGGTGVIATIKGANSGKTVALRGDMDALQVVECTDVEYKSKNEGLMHACGH
                     DGHTSMLLGAAKVLNDIKDSINGTVKLFFQPGEEVGKGARAMIQDGAMEGVDSVFGIH
                     LWTDVESGTISVEEGPRMASADFFKITVKGRGGHGSLPHQGVDAVLASSAIVMNLQSM
                     VSREVSPLEPLVVSVGVLNSGTRFNVIASEAVLEGTIRLFNPELRKQIPGILERIAKS
                     TAGAYRADAELEYGYLTPAVINDKECSKIATDAAIKLFGEDCITLFEKVTGAEDLAEF
                     MNIAPGALAFVGARNESKGACYPHHHGCFNIDEDALEIGTALYVQYAVDFLNK"
     gene            complement(21060..22247)
                     /locus_tag="KRL67_001514"
     CDS             complement(21060..22247)
                     /locus_tag="KRL67_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH1522402.1"
                     /translation="MEAITAFCLVLISLTIGDIVSAKTKAFVPSVFVSAIIFIVGFWT
                     FFPENIVDLAALGTPLAQLGMLLLITHMGTMMSIKELAGQWKTIVIALAGIVGICVGA
                     LALGTVVFGWDTAVIATPPLTGGLVASIMMADAATAKGLASLSVLAILMYVAQGFAGY
                     PITALMLKKEGKRLLSDFRSGKVTVNTAEEKVKDLPEQKSRFKIIPDLPEKYDTTYMI
                     ILRLGFVAWLAVQFTNLTNEVVSRYVICLIFGVIASELGIVDRKPLNKSGSFGWLMTS
                     LMAFIFAGLAKATPAMLVEMAVPLVGIIIFGVIGMGIFSIIVGKLLGESKEMAFSVSL
                     TSLYGFPPNYILTEEAVKALAETPEEKEFLMDKMLPKMLVGGFTTVTIASVVIAGIFV
                     NLI"
     gene            complement(22610..24310)
                     /locus_tag="KRL67_001515"
     CDS             complement(22610..24310)
                     /locus_tag="KRL67_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBH1522403.1"
                     /translation="MIKQFVKYYKPYKKIFTLDLIAAFLFSLCDLVYPMITRNIMDDV
                     VPNKNLRMLVVFAVALILIFIAKAGLNYFMQYWGHVIGVDMQADMRNEVFTHLQRLPN
                     TYFDNNKSGVTMSRIVNDLMDITELAHHGPEDLFISIVMLVGSFFILIDINIPLTLII
                     FAILPFIIWFAIAKKDKMNIAFMKSRVTIGDVNATLENSIAGMKVTKSFCTEKEELNK
                     FVRSNKLFRRARQDSYKVMAEYYSGMNLYMDILEWVVVIAGGYFTYIGKITLGDFAAY
                     ILYVKMFIQPMKKLINFTEQYQNGMTGFKRFIEIMEQDHQKEAKNPIELENVKGDIEI
                     ENISFTYEDKTQVLDNLSLSIKAGKTIALVGPSGGGKTTLCNLLPRFYEFDKGDIKID
                     GKSIKDVSLKSLRKNIGIVQQDVFLFTGTIRDNILCGNPNATDEEMIAAAKKARIHDF
                     VETLPDGYDTYIGERGVKLSGGQKQRISISRIFLKNPPIIILDEATSALDNVTEREIQ
                     ESLEELSKDRTNLVVAHRLTTIKNADEIIVLTDNGIEERGTHEELVNKNGVYSRLHNN
                     "
     gene            complement(24553..24828)
                     /locus_tag="KRL67_001516"
     CDS             complement(24553..24828)
                     /locus_tag="KRL67_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBH1522404.1"
                     /translation="MKKILVACGAGIATSTIVCDRVERLVKENNIQAEVEQCKIAECS
                     TKQEGADLIVSTTILPTTYDIPTIKATGYITGINTTALDKKILNALK"
BASE COUNT         8561 a         4113 c         2664 g         9567 t
ORIGIN      
        1 cgatattatt ttaaagtatt tgtcatttaa aacaaacttg tgataaaagc atatctgaaa
       61 gtattctgat tctaatactt aataaagagt tctttttaca gttatagata taaacaacat
      121 ctatcttaat agactgtata gaggcacaat caaaggtaaa cacatatagt taagtaatat
      181 atccttgatt tttctttatt ataatagctt tattaattca ttttaatatt acatcatttt
      241 aaaaaaatta tatgaaaagt caaaatttac atatagatta attaaacttg taaaaacaca
      301 tctaaattca tacaattaag tacttaaaac ttgaaaatac ttactttgct atatatttta
      361 gataaagata taattgattt ggttaagaaa ggagtctaat atgatattca gtagtttaat
      421 atttttattt ttattctttc cagtcacact catttgctat tacttggcaa atcctaagta
      481 taaaaatgtt atgttacttc tagcaagtat tgcattctac gcatggggag aaccaaaata
      541 tatttttttg atgatactat caataatagt aaactatata tttggtatta aagtttctgg
      601 cagtaaagat aaaaaaatgt gggttgtgct ttctgtgatt tacaatatat ctattcttgc
      661 tgtatttaag tatagcaatt tttttataga taatattaat tttttattca atacaaatat
      721 aactatacct gaggttaaat tacctttagg tatctcattt ttcacattcc aaataatgag
      781 ctatgtaata gatgtatcta gaaatgatgc taaagttcaa agaaaattag ctaatttagc
      841 attgtacata agtttattcc ctcagcttgt agcaggtcct attgttagat atcagactgt
      901 ggcagatagc attgagcata gagaacatag ttgggacaaa tttactaaag gcgtaaacag
      961 atttgttgtt ggtcttggaa aaaaagtaat tctggcaaat caattaggta taatcgcaga
     1021 tggtgtattt aacaagtcag ctggagactt atcaagactt gaggcgtggc taggtatagt
     1081 ttgctataca cttcaaatat tctatgactt tagtggctat agtgatatgg ccattggttt
     1141 aggtaagatg tttggatttg agtttttaga aaatttcaac tatccatata tctctcaaac
     1201 agtatctgaa ttttggagaa gatggcatat atctttaggt acttggttta aagactatgt
     1261 gtacttccca ttaggtggaa gtagagtctc taaggcaaaa ctatatagaa atctatttgt
     1321 agtatggttt ttaaccggca tgtggcatgg agctagttgg acttttatta tatggggtct
     1381 atattatgga atattaatat cagtagaaaa agcattctta gaaaagtttc tatataagat
     1441 gccaagaatt gtaagacaca tatatctact tcttatagta atgattggat gggtattttt
     1501 tagagttgat gatttaccag cagctattaa atttataggt attatgtttg gtgctggaaa
     1561 ttatccagtt atcagtaatg cattcaatgt atttataatt gaatattatt atgtaattgt
     1621 tggagcaatc attctatcaa ctcctattgt aaaagttgta aaatcattat taattaagaa
     1681 aaataacaac acattaaata gcacattata tcaagttact aatgcgtttt tcattatttc
     1741 ttgtatgttt attgtaatag taatgttaag tagctcaacc tataatccat tcttatattt
     1801 tagattctaa tttggaggta taaacatgag taaaaaaaaa caaggattgt tgccatttca
     1861 tttttaatta tagtatttgg atttttcata ctaaacttca cacttccaaa gaaaaaatta
     1921 agtataactg aaaatagaaa tcttgcaaaa tttcctactg tagaaaattt aaaaaaaggt
     1981 gactttatag ccaagtttga agaatatttt aatgaccatt ttgcatttag agatgaaatg
     2041 atatcaataa atacaaagac tcaggctagt ttaaataaaa ctaaggtagg aaactactat
     2101 ctaggaaaag ataactggat attaggtatg ttttcagaaa ttttagacca aaaacaatta
     2161 aaccgatacg aaaagtccat taattattta tctaatctaa gtaaagatat gggaaaagat
     2221 gtgtatttta caatgatgcc acataaaact aatatgttaa aacatttgta tcctaaatat
     2281 gttgataata tggataatat agacataaat ttaaagaatt ttaaaaatag attaaatcca
     2341 gatttaatta aatatataga catggatgag tattttttaa gtaactattc agataaagaa
     2401 agagaaaatt tatattttaa aactgaccat cattggactg gatatggagc atttgaaggc
     2461 tttaaaatga tggcatcaaa tatggactta agtctatcta agcaagattt agacaagcat
     2521 tttagtagct ataaaaagtc tgtaattact gataagaaat ttataggaag ttataatcaa
     2581 aatatagata tgctaattaa agaaaatgaa tatgtagttt atatgacaaa aggtaaccaa
     2641 gattatagct ttgatataaa tggagaagaa aaagaagaaa aagaagttta tgcaactgaa
     2701 agagataata aagagtggga ttatggtggt gcatatatta gaggagctaa aactaatata
     2761 ttaactataa aaaaccctaa agcattagta gataaaaaaa tactaatatt tagagattca
     2821 tatcaagctc ctacaacact tctacttgct gatatgttta aagaagtaga gatagcagac
     2881 cctagaaata taaactcaat agataaaaca tacaaagaaa tgattgagga aagtaacgca
     2941 gatattgtaa tgtttatgta taatagtttt gggtttgccc aaatgttaga tgcaatgata
     3001 gataagggaa taaagtagca ataaaaagcc tctattaaac tttaagttta atagaggttt
     3061 tttattgttt attttttatt actttggtaa aaataaacta atcaaaataa aagcaaggag
     3121 atgtagcata ttgaataaaa gatataacct acttataata tcagttatat tatcaatagc
     3181 tttaattaca tttaataata aacagaataa tattacaatg aaagaagttt ttaatgccca
     3241 tatcactcag aagcaaaata aaaagattaa acaaaaatat tatctaacta aagatggatg
     3301 gctattaggc aaatttgcta aattattacc tgattatcat ataaagtctt actccaaagc
     3361 cattaataac ttgagtaact taaatagtga aaaagatttt tactttgttt cacttcctca
     3421 taagacaaat atgcttaaac acttgtatcc aagtggtata gaaggtaaaa ataatataga
     3481 tattaacaaa gaaaacttag aaaagcaatt aacagatata aatttcttag atacagatga
     3541 ttacttttat agaagtttta cagaaaaaga gagagaaaac ttctttttaa aaacagacca
     3601 tcacggaaat tcgttaggag catttgaaga atataagttt attcttgata aattagacct
     3661 tggattatct aaatcagaaa tagataatca tttaaaaaga tacagtactc gatatgtaaa
     3721 tgacaaaatc tttataggta gttataacaa aaaaatggga tatccactta agaaaaaaga
     3781 gtctataagt tatgttgaaa taagtgatgc aaagtacaaa tattttatta gtaatggtac
     3841 taaaaatact gagattaatg aaaatctcat aaaatcaaaa ttaaaagata aaaaagaatg
     3901 ggattatggt ggagcttata tgagaggttg tgattgtcac atacttaaga ttagaaattc
     3961 aagctcttta actaataaaa aggctcttat atttagagat tcgtaccaag cacctatgac
     4021 ttggatgttg gctgatactt ttaaggaact tgaaatacta gatcctagat atataaaaaa
     4081 tatagatatg acctatgatg agataattaa aaacagtaat gcgaatgtag ttttatttat
     4141 gtataatagc tttggatttg ctggaatgat acaagaaatg attgacaagg gaatagcata
     4201 acaatttgaa tcaactcctt tttatacaaa aaatagttct caataattcc taatctatat
     4261 gataatttag tacatctctt taacaataaa aataaaatca caaaaattct tataccatca
     4321 cttatcctta tgataaattg gttgagaaaa aattttataa tttatatgat atgcaatctt
     4381 aattaattct ttatttttta attctttatc tactgcaacc tttgataaaa gaaaccatcc
     4441 aagactatta atatgaattt atataaaaat tactttcaaa taaagtactg cagtattttt
     4501 atttaaagta tctataatct ttaaccacaa aaagccacaa aatacaatgt atacttatgg
     4561 ctttttataa atttatataa ttgtatataa agatatattc taaaactgtc ttaagaatct
     4621 aaagtttaaa aatccaatat ttttattgtg aatttaaact ttttattaca actttttatc
     4681 taatttattg tatacaacaa cactacaaat aaattgaaat aaaataagca atccaagaac
     4741 aactaaaacc cacttgctta ttaccattac agaaaataca ctcaatatag aaacaataaa
     4801 tataagaatt acaagaacat aggacatgat ttgattagtg atacgtccag ctttatcacg
     4861 taacataact cttctttcat cattcatctc tatcctttgg ttttttatct tttcttcata
     4921 atctactgct ctttcaggct tactccaata catatattga agaatcatac atatacctgg
     4981 acctaaagca gctccagata atccccaaat aaaagcctca aacgaaaact cagtaaacgt
     5041 agccaataaa acaagtaaaa ttccacaaat aaaatatact attccaagat ataacatatt
     5101 ctttttcatt tttactcctc ctcataaata aatatttctt caatattcat attaaagtat
     5161 ctagctatct taaaggctaa gatgatagaa ggattatacc taccattttc taatgaacct
     5221 atagtttgtc ttgatacttg caaaacagag gcaagttctt cttgttttat gcctctttct
     5281 tttcttattt cttctaatct atttttcaca tctatccccc tttaatatct caactatgga
     5341 aagtctactt tccataatta tacaactata ttccatttat gtaaagctta ctttccatat
     5401 ttacaaaaaa ataacagact attttcagcc tgtaatttta aaatttcatg ttgtttatta
     5461 aatttaatgt tatttattaa ttccatatta tttattaact ttaacttggt ttctgaattg
     5521 gaatccaaac ttctgtagta taatcaggag aattgatatc gccattggga taaagttcaa
     5581 tatctggtaa attagcatac acatacccag aacttggtaa ccactcagta ataattcttt
     5641 tttgcaactc ctgtattgcc agagcatttg gcattggaga aacacattca aatactgccc
     5701 atgtacctga tggtattaag tattcatgag tatcttctaa tacaggctta tctgttggtg
     5761 cagcaatata ataatctaag cccttactat ttgtacacac actaactcca agcaatccac
     5821 aaggttcgtt atttattaac tctgaaagtt tatcaattgt tccatttttt atgcatttag
     5881 cccataactt aggaactttc tcaaaacact cttctacaat catgttcata ctttctttta
     5941 taccaacaat acgaaatcct ttcttttcta caatacgata ttgcatttct acatttcctt
     6001 ttacataaat gcttaatgtc atcttaggat atgcttttaa aacaacaccc tttgctctag
     6061 ctgcagatgg agaaatgtta tgtatatttt gaaaagctct attaaaagat gttggtgaat
     6121 cgtaaccata cttctcagat agttctaaaa ttttgatgtt actgttctga agttcaaatg
     6181 cagccttagt catacgtctt cgtctaatat attcagataa tggtacttct gcaatatatg
     6241 aaaacattct ttgaaaatga aaggttgaac aacatgcaat ttttgctgct tctgcataat
     6301 ccactccact ttcgagattt tcttcaatat aagtaatagc ttgatttaat ttatttatcc
     6361 attccatttg atattacctc caagcaaatt atacagttta ataaaataat atacctctct
     6421 attcatgcac aaaattgtaa gcatttaaat tcataagtat ttttcaatca atacaataaa
     6481 tataaaaatt gtattataca ataaaaaagt tcaataacat tatattataa aatgttattg
     6541 aactttttat aaaaattaat ttaagtgtag ttaaattaag tattacacta tcttatatat
     6601 tacaatatcc tactatctta tagtcttctg gcactttata atctttgtct ggagtaccta
     6661 aattccattt taaatcaaac atagttgcac ttattattgt tgcaaaataa agcataacta
     6721 taccaatgtc tattttttct tcatataaat tagtgtgacc atcttttcta acagctaaaa
     6781 ctacagtgcc tccatcaact ataaatctcc atggttgtct atttaatgta gatggagcta
     6841 atcttgcata gtgaaatgca tcaagaagag ctctttcttc taagatttct atacttgcaa
     6901 catttcccca ttcgttcata taaacaacat cttctacacc tagtctagta gaccccttat
     6961 ctattacaac ttgcatatca gattttgaat aattttgccc agtttttgta gcatttaata
     7021 ctttagcatt acttgcattt tctccatatc caatagctat aattcctgtt acctctttat
     7081 ctgaattaat tccaagtttt tcttttacct tttttccatc ttcaaaagta acccaacaag
     7141 aaccaattcc aagttctgtt actttaaaga ttatactttc tcctatgtac cctgaatttt
     7201 caatgtaatt atcttttatg tcagataata ttataatata acttggagaa tctatcatat
     7261 ttcctttgta ccctgctact ttatctaaat tattaaaaac ttcacttcta ttcataattc
     7321 ttacatcaac atctatttct ggaactagtt tcttacaaga ttctgagtat ttcttaattt
     7381 cactaagata ttcttcttta atatctgtgt tcttatattc tctaacagac tttctattta
     7441 atatgattgt tttataatcc ataattttat cctccccaaa ctagtattgt tttaattata
     7501 tacccattaa tatattattt tactcattta tttaataaat aaagattaca attttgtaat
     7561 tattatttat taataatatt ccacttttac attaataatg accagttgtg caaaatccat
     7621 tgccaaccac caaataaaaa catattataa aggtaatata aaataacaaa caaatcttaa
     7681 caaaaaatta aacttttttt acacgaaaga aggcttcccg ttgccttctt tatttttata
     7741 aaaaataagc tgtttctaaa atgatttcac atttttgaaa cagccaagtt attctataat
     7801 gacaactata atttgaggat gtgagggaga gttgtcatta taaatttagg gtaattatat
     7861 tatgttttaa gctagttctt tctatattta tataatacat tttatatatg aattttatat
     7921 gagattggta taaatgatat atgaaattac atatgtttat attatattaa attttattat
     7981 tacattttcc acaacaatac tcatatgtct ccacattatt tggaatattt acaaatacaa
     8041 attctttaca gttctcacaa tactttaata tagatatagt tccacaagtg taacattcaa
     8101 atattacaca atcctcattt tcctctacta tttcaaactt tttactatgt tcaccacact
     8161 ctgggcactt gtgattattt acattcataa atactcctcc ttattagtat agttacatgt
     8221 caaaaactta attaatgtat tctatattac ttattatata ttctataata aatcatattc
     8281 taatataata aaacaaatta aataaaaaaa aaccataaat tttaaattta tggattaatt
     8341 cttgtaatga taactctatt tgaggatgtg agggagggtt atcattacaa tagggtatta
     8401 tattatggtt tttaagctag tctttctaca cctcatatga tacatatgct atatgaattt
     8461 tatatgattt ttacataaat attttatgaa atttaaattt tttagtcatt ttgaaatttg
     8521 taaaaagttc tctaaaaatc cacagcatca taaacagcca aaatttaagt aaaacatcat
     8581 aaaaaaattt gtccaataat tttaacaagt aatctcacct cattataatt attgacacaa
     8641 acttaaataa acaatctata aatcttctat aaatcttaaa ctttccccct attgatacac
     8701 aatttctagt ttgtcaattt tattttgcat ttctaagaaa atttcttcaa cagaattgtt
     8761 aaatatattg cctaaataga ttcttttatc tttttgaaat gaataagatt ttacactacc
     8821 atcagaagaa atatataaat gactccactc tgctccatat tttgaaaacc tctcctttgt
     8881 atctttaaac ctcatatctt ctatttttat tgctttttct ctgtctcctc ttactacagt
     8941 gtgacccctt tttattaaac tggttatttt tatatcttct tttaatttaa tacgtgtatc
     9001 atcatttttt gaatatactt tccatatcac tttatgacga taattatcta attcctttat
     9061 taaacttcta tcaatagtaa agctatcttt agttaccaca attaaatcaa aatggtctaa
     9121 catatattga aatatttctc tcatactttt attttttaaa atatcctcac caataacctc
     9181 tataaatcta cagcctttct catacatgca gttcaataaa cttgtgattt cagaaaaata
     9241 atcatttgta ttattatctc catctatttc tatagtaaag tacaaaggag taatgtatga
     9301 tttatttcct gttatctcaa atacacttga tagtgtttgt atatcatctt caaacgttaa
     9361 gtatacattc tcataaaaca taaaaaattc cataactttt tctttcatat tatggatatt
     9421 tctatatcta ttggataata tatttattat atcgtcagta gtatttttcc catcacattc
     9481 agccagtata acaatttgct tccaacttaa gctatccaca tttccgatac tttttttata
     9541 tccatcttct tcatcataaa taggaccaat tataatgttt tcttttggcc acagatgaac
     9601 cctagttcca tcttttaatt tcggatattt attttttaca gactcataaa atctacttgt
     9661 atcacccatg attctttact accccccata gtttaaatat tctatgctaa aaatagcact
     9721 ttatttttat taattcaaca attattcaaa attggtcatt atatatgttt aaatggattt
     9781 aacatcaacc ttatttatat gttattgatg tttatatatg ttatataaaa tcttatttct
     9841 catacttcaa tgtaatctta actatttctg gtttacaatt aagcctaatt aaaacttttg
     9901 agcctcctaa gcctttattt aaaatcatag tgctattatc tttttcatat tttccccaat
     9961 catacttagg aaagaactct ctatttggag ataatacacc tccaacaaaa ggtattctta
    10021 ttatacctcc atgaacatgt cctgctaaaa tcaaatctgc tccccatttt gcatattttt
    10081 caaagaaaaa tggtgtatgt gctaaaagaa tgttatattc attttcatta actttaccta
    10141 gattgtcttc tacaaaattg tcacttagtt ctaatttttt gttattagca aataaatatg
    10201 gatagcattc caatggtata attaaaccat ataaattaag atgactatta cctctatata
    10261 ttcttacaga ttcattttcc atatttatta ttttcttcga gtatagttcc ttaaaataat
    10321 ccttatatag atgtttatac ttttttatta aagatttttg ttcatgattt ccaattatat
    10381 gatatacttc atatttttca accaaattat ctattaaatc taatgcaact tgaaaatttt
    10441 tattttctcc atctactaaa tctcctgtta taaatataac atctggattt aatgattcaa
    10501 ttttatctat caatacctta ttatttttat caaacacctt attatgtaag tcactcactt
    10561 gaactatttt aaagttatca aattcctttg gaattttttt gtttaaaata gtatattctg
    10621 tcgtttctat atgctgagtt tcatacttat acagaatata caataatatt aatactaata
    10681 ctgtcattga aacctttata atcatattct cttcctcttt attctattct attctatttt
    10741 attaatatta ttataatgct tttatgtttt ttttaaaaca ataacatcat cacatacata
    10801 taaatatttt cgacattata aaaataagtt atccctttct aaaattttca tttattttag
    10861 aaaaggataa ctataaaatt atctataaac ctctatattg tgaaatatag tttttccttt
    10921 atttaaattt ttatatacat gctctaaagt tccatcaaac ctaatagaat gtccagactc
    10981 taaaatataa gttttcccat ccacattaag gtcaagttta ccttctatta cgagcacata
    11041 ctcttctaaa acatgactat gtgtagatga aacatgagta cagttttctt caagctcaat
    11101 tataaaaagt tcaaaccttc tttgagggct aaaagggtat attggatata acttcatgtt
    11161 ccctttttgt tcaacaatag gctctacttc ttcttttttt ataacctcat aggtatttgt
    11221 attttcattt aatagtgtag taaaatttat tttaaatcca gatgaaattt tccataaagt
    11281 agatactgtt ggacttgatt cacctctctc aatctgtcca agcattgctt tactgacacc
    11341 tgtcatcttt gaagctttgt ctaaacttat gcccatttct tgtctcaata accttaaatt
    11401 ttctcccaca ttaggaaatt tattatccat gttaaaatcc tcctgatagc tatttgtatt
    11461 ctatgatttt tactcctgta agttctacta tctcttttaa ttttaacata tgattgttac
    11521 aaatttcatc aaattcatat atataatctt caagccctaa tgccttatat ttatcgattc
    11581 caaatttatg atatggaagt aactccattt ttcctcctgg aacattttgt ttgacaaata
    11641 atgcagtact tcttatgttt tcttcactat catttactcc ataaataatt ggcactctta
    11701 taaccataga tttattcaac tttgaaagcc tacatatatt atctaatata attttattac
    11761 ttactcctgt atattcttta tgaatattat catccataga ttttatatct acaaatatgt
    11821 ggtctatttt ctcaaataca tcatttacct tattccaatc aaaatatcca caagtttcaa
    11881 ttgcaatatt tatgccttta tcataaaaaa tatcaactaa ttctcttaaa aaatcaatct
    11941 gaagtgtagg ttcacctcca gaaaaagtta tccctccatt agattcaaag aaaaatataa
    12001 aatctttttc aacttcctct actatttctt caacactcat ttcttcggtc attatacaaa
    12061 tagcatcttt aagacatact ttgatacact ctccacataa atcacattta ttatttattt
    12121 gtgttctatc atatagtgaa cttatatttt gaggacaaac atctatacat ctattacagg
    12181 atacacattt atctttcata acacctaatt ttactatatt actccaggaa tctggattag
    12241 aacaccactt acattttaat ttacatcctt ctaaaaatat agtagtcctt attccatctc
    12301 catcatttac tgaaaaatgt tgtactttta caactctacc ttttttactc ataatctacc
    12361 tcataattaa aaattgctat gtgaatttct agctattata gcatcttgca tttctcttga
    12421 taaattaaca aattgagtac tatatccagc aactctcact aataaatctt tatattcttc
    12481 tggattttct tgagctttta atagagtatc atttgatatc gtattaaatt gtacatggaa
    12541 tcctcctaaa gcaaaatatg atttaatcat agatgttaga tttctaagtc ctctttcagt
    12601 ttccaccaaa tcttgattca gtcttaaatt tagaagtgtt ccaccagtat gaacatcatg
    12661 atttattttt gatgcagatt tcattgctgc tagtggactt gttgtatcag taccaacaaa
    12721 tggagaaact ccctctgtca aaggttttgt cgcttttctt ccacaaggag ttgcacctac
    12781 gatttttcca gttggtacat agtttgatat acccataaat gcactattaa atttagagcc
    12841 aaaaatatcc ttgtattttc tagtttcagt ataataaaaa tcagaaactt caattgctaa
    12901 tgaatctaca taatcattat catttccata cttaggaaca tctaacgcaa gttttcttaa
    12961 ttcttcataa ccttcccaat cattatttag tgcttttgtt aattcttcta gcgtagtcac
    13021 cttatcctca aatactaatt tttttatagc tgccaatgaa tttgaaacta cacctaaccc
    13081 tataccagtt aatacaggac ctatattata ttttgctcct cctttactta aatcaactcc
    13141 cttatccaag catccatcta cacaagtaga taaaaatggt cttggtacca tttctttatg
    13201 aatttgttga gcaacagtag ttcctataac agaacttttt acaagatatg ctaattgctt
    13261 taaaaactca tccttaagtt cttcaaaaga agtaaattct gtgatatttt tcgcatctag
    13321 ccccattttt tctccagtca atctactctt accttcattc atagcatatt ctaaagctgc
    13381 tgcaaaattg atatttacgg ctgaagtcca ctgtcctgtt tttctaaaat gaggaactac
    13441 acaaccacag ttactccaat ctctagcatc ttctgcatca tatccatctt gtagcaacat
    13501 ttgtgcccct gcactatcgc tgtgtatagc agggaaacca gttccttgtt ttaccaattt
    13561 agcaactgcc attacgaaat tatctggtgc tccttgactc accctaacac ttaatcctgg
    13621 ctgatgagtt tttacacttt ctgtagcctt taaacacata tatgagatat cattcgttcc
    13681 atcagtacca tttctctttt taccaccaac agtaagattt tggaattgat tatatcctgc
    13741 aaaataattt gcagtatttg ctgatatggt ccatacccat tctgataact taatccaaag
    13801 tgcttctatc aattcttgag cttctgattc agttattttc ccttctctag catcattttc
    13861 ataatatgga tacatatatt ggtcaaatct acctaaattt aatgccaatg gattttctga
    13921 tagaatacca cctaattgaa caaaccacac aaattgtatt gcttcataaa aattagttgg
    13981 aggatttgct ggtacattca tacatatctc tgaaattttt ataagttcta actttctctt
    14041 ttcatctgtt tcttgcttac tcatctcttt tgcaagactt gaatatcttt gtgccaatgt
    14101 cataataccc tgtgcaacta tagaaacaga tttgtaaaaa tctatctttt caatattttc
    14161 tgaaactgat atatctaatt ctttaatttt tttatctgca tcttccttaa tccctttata
    14221 tccttttaca aacaatatat cttgataatc tggtgttact tcaccaactg cctgtctcca
    14281 cttagaatca ttatctatta ttcctgtatc tactaatatc ttagctgtat cttctgggat
    14341 tcttgctaag aaaacttctt ccaaagattt tcccttccaa tatggaaata tttcttttct
    14401 tataaattct atttgttctt ttgatattac atatgggtct tgagttcttt tatcaaaagt
    14461 atccatttct ttgtctaccc attgccaaga aaactctggt gtcaatatcc ctgttcttct
    14521 aaattttcct gctgtaccta ctattaattc attttcaaag atttttatag gaagctcttt
    14581 acatgtctcc aaaaaagctt tagctcttct tatacagata gcttctccct cagttttttt
    14641 atgtgattct gtaaaaattc tagccctttc acaacatatc ataggtttag aattaatata
    14701 ttcttgtcta aatgcttcta ttctattagt ttgactcatt taaaatcccc ctttggataa
    14761 tatatactac atttttgtat gttataacat actttaatta tattatactg tcctttagca
    14821 taaaatggaa gcaaaataac ttattttttt tcatctacct tgaacacata tatattaata
    14881 ctttttttgt taaatttata aaatctaaaa aaattttcta tgatatattt tctatattgt
    14941 tatagttatt tctaatatac taatgatttg aaatatcaaa tttgtatact atacttagtt
    15001 ttaaaacctt ggttgattgt ttaactttgt taacttcaac atgtatatcc ttcatataag
    15061 gaagctctac aacacctact cccttattaa attcttcttc aaccatagaa aaaatttctt
    15121 tctcagttaa gttcgtatta gtcaatgact taaataaatt gtatataaat ttaatatatt
    15181 tatcactctc agataactta tcatcttctg gatactcttt agcacagaga atatctattg
    15241 ttacttttcc ttctttatcg tcttcagaca tagaatagaa taaacttcga atattttttt
    15301 caggtggttt ttcagcacca tttatagata ccataaagct accatctgat tttgagtcag
    15361 taatactata accactgtct attatagctt tcgaaaatct ttgagataaa tcttttttta
    15421 cttcttctct acctttcgca tcattaatct tttgtgagta attattaaat agctcaatag
    15481 attcctcttt tttatcttca acttgttctt tcaatgaacc tttactaatt tgttctgagc
    15541 tacttaagtt cttatcagat acaggagaca tacatcctac catcaatgct aagctaagat
    15601 ttaaagccac aattattgcc actctattta tattcctttt tactttcata cacaatcctc
    15661 ccatcacact aaccgctcta aagtattact cattgcacta gaatatgttt atattctctt
    15721 tatattaaag atttattatt caatacatat caccttccta tccaattctt ataacttact
    15781 attagtaaaa ttataagctt cttaatacag aaataaggat gttatccttt gttattaaaa
    15841 gataacatcc ttattttttt gtatcaatac atatgtccca actagtaact taatcgcccc
    15901 aactgtcagt atcttggtcc aatggtatat atatatttag tataaattta tcgatactat
    15961 tttcaaataa taattctcca ttatattttt gtaaagaaga tttaatactc tgaataccta
    16021 ttccatgtac gaacttatcc tttttgtttg ttaacaatat atttttatta aaagtaagct
    16081 gatttagttt actattttca catttaagta caaaaaatga ctttgttata gttcctttaa
    16141 ttctaatata tttattaata ttttcatcca ctattttatc acatgcttct attgcattat
    16201 ccaaaatatt agaaaatatg cttgacacat ctataggttt tacaaaattt acttttgaaa
    16261 aatttataga gcaagtaaat ttaattcctt tttttaaaca tatttcactc ttttcgttga
    16321 ttataatgtc taatatcata tttcctgtat tatagatata tttaaatgtt tttatttcat
    16381 cttttaaatt gttaacatat tcatcaattt ctttgctatt atttctaagg ttgtctatac
    16441 aataaatgtg attatttata tcgtgatata gtttcttaac tcgcatatga gattcttgaa
    16501 tacttaaata atgtgcatat tgcatatcta acttattttt tattaattca gcttgggatt
    16561 ttaatctatt ctcttttatt atttctttaa acaatagtat aagaaatata ctactagctc
    16621 ctaccaattt gggattaaca aaattatcaa aatagtatcc ttctgaatgt aatgacccaa
    16681 accgttctaa tttttttaat acataattta caaacatatt cattgtatta gctaataagg
    16741 caaataataa atatacatag tttctcttat cttggctaat taattttata gacttcctaa
    16801 aaaagctaaa tataagatat ataagaataa ttattaaatt actatatatc tgaattttta
    16861 tagattctaa gcgaggtact aataaggatt ctttagatga aataacatta cctgtaattg
    16921 caaaaaatag tattctatat ataacagcat ccaaacattt ataatataca ctaaatagat
    16981 aaaagaaaac ggaatactta atataaccta tcttataaaa ataattatag tatcctcccc
    17041 aagcaataaa tgctaatata taaaataaat agtttatatt actaaagtaa tttcctccat
    17101 taaataatct acttatctga actataatgt aaataagaaa caaaacaata taaaatacta
    17161 ttttttcatt ttttttacta tcctttttat aaattaaaat tccgttcatt atagaaaact
    17221 gtataaatat agataaaact actaatagct cagtcataaa atttagcata ttgtatcacc
    17281 aagaaatttt aaaaatttct ccttaacttc tttatatcta tatctactaa taggtacctc
    17341 ttctccactt tccaatatca cagtattagg ctttatattt tcaacatatc ttaaatttac
    17401 tataaaactt ttatggcatc ttataaattt atatggattt aattcttttt ctattttact
    17461 taaactatat tttatatcaa aattcttgtt tattgtgtgt attagcatat cttttttttg
    17521 aacttctata tattttattt catttaaata aatcttatat gtattagact tattttttat
    17581 tgtaatatga ctttccttat ttatttcaat atctttgata caagctagca catgtttttt
    17641 tagttctttc aactctattg gctttagtaa atacctataa gctcttacct cataaccttc
    17701 ttgcacataa tctattagtg aagttacaaa tattatttcc atttcattgt ctacttctct
    17761 aatctttctt gcagtatcca ttccatttag tttttccatt tttatatcaa gaagtaatat
    17821 atctatatct tttaaattac tttccagtaa ctcttctcca gatatatact tttgtatctc
    17881 atattctata cttatatctt ttaatatatt ctcaaggtaa tcttctatta aaagttgtgt
    17941 ttctttttca tcttcacaaa ctgctatctt aatcataaaa tgcctcctta cataatttct
    18001 agttaataca attaaaatat ttataatata tttaaagata attttgtata tctgatactt
    18061 taggataaca ttttcaaatt gtttttatca atactcaatc ccatataact gttattttga
    18121 tatgtcaatt taaagacaga aattactata attaaaaata gtacttactg ataaaagaag
    18181 atgtcttaaa atattaaaaa aataatttta ggacaccttc ttttatttat ttaaaatttt
    18241 taagccttaa ataatacatt aatcgcctat ataattgtac tgcatataaa atcaacaata
    18301 taatctgtag tacaatagca gttacagatg ccccataacc actatataaa tttcttattt
    18361 caatattcaa taaatatata aatattacta ctaacataaa gaatataaac atattcataa
    18421 ataaaattga ctttacttta ttttttttat cagaatcact atataattta ggaggtatgt
    18481 ctttgtattc tttcgaccat atatactatg cagatgtacc cataaaacta ccaatacttc
    18541 taaatacaag attccagcca cttgatttat gttcatcaaa ataattattc tctgcatcat
    18601 agtagtaatc agcagtatat tttatttttc ttgggcttcc cttatgaaaa taaaatattc
    18661 ctccaaattt atttatccta tataaattat atcctttagc ttccatatct tctaaccatt
    18721 tctctatctt atcaggtgag ctataccaat caaatttgaa ttttattttc ttttccataa
    18781 tactcactct ccaaaatgat atttttaaat agcttaaaag tctatttaaa taattatatc
    18841 attttgatac taactttagg ttacaatcag atatttaaaa agctatttaa ataatagtaa
    18901 actaatagcc ataacaccca ttcctgcaat caatccataa atcgataaat gatgttcacc
    18961 atattctcta gctgatggca ataattcatc tagtgatata aataccataa ttcctcccac
    19021 aattgcaaat acaactccaa gagttatatc atttaaaaaa tttctgagaa gtgcatatcc
    19081 aataattgct cctaaaggct cagacatacc agataaaaag gaataataaa aagctttctt
    19141 tttatctcct gtggcataat aaactggtac agatacagat ataccttctg gtatattatg
    19201 tattgcaatt gcaattgcta tcgggatagc tattgtaaca tcatcaagag ctgatataaa
    19261 tgtagctaat ccttcaggaa aattgtgaat tgctatagcc attgctgtaa atatacctgt
    19321 tctcaaaaga gatttatttt ttttgatatt cttttcttct atattctcca ttttttttat
    19381 ttcatgtgga ttttcttctt gtggaattaa cttatcaata acagcaatga tagccatacc
    19441 tataaaaaaa gctattacgg ttgaccaact tcccaatttc tctcccatag attttgtcaa
    19501 agcatcttgt gctttaggaa aaatttctat catagaaaca taaatcataa caccagcaga
    19561 aaatcctaag cttatagaaa gaaacttagt atttgttttt ttagcaaaaa atgctataca
    19621 acttcctata ccagtagata aaccagccaa taatgttatc aaaaatgcaa atattacatt
    19681 attcaaataa atcaccttct ttttaattga tattgattat cattttctta atgctatcat
    19741 atatatttta tatagtcaat atttataaaa aatatacaca ataaaaaagc agagatataa
    19801 acttagtctc tgctttttct ttatagtata ttaaaattat ttattcaaga aatccactgc
    19861 atattgcaca tataaagcag ttccaatttc tagagcatct tcgtctatat taaagcatcc
    19921 atgatgatga ggataacaag caccttttga ctcatttcta gctcccacaa atgctagtgc
    19981 accaggtgct atgttcataa actcagctaa atcttctgct cctgttactt tttcaaatag
    20041 tgttatacag tcttctccaa atagcttaat tgctgcatct gtagctatct ttgaacattc
    20101 tttgtcattt ataactgctg gtgttaagta cccatactct aattcagcat ctgctctata
    20161 tgctcctgct gtagattttg ctatacgttc taaaattcca ggaatttgct ttctaagctc
    20221 tggattaaat agtcttattg taccttctaa gacagcttca ctcgcaatta cgttaaatct
    20281 agttccagaa tttaaaactc caacactcac aactaatggt tccagaggac ttacttctct
    20341 actaaccata gattgcagat tcataactat tgctgaactt gcaagcactg catcaacacc
    20401 ttgatgagga agtgaaccat gaccacctct accttttaca gtaattttaa agaaatcagc
    20461 agaagccatt cttggccctt cttctaccga tatagtccca gattctacat cagtccaaag
    20521 atgtattcca aacacactat ctacaccttc catagcacca tcttgaatca tagctcttgc
    20581 acctttacca acttcttctc ctggttgaaa aaataatttt acagttccat tgatactatc
    20641 tttaatatca tttaatactt ttgctgcccc tagaagcatt gatgtgtgac catcatgacc
    20701 acaagcatgc ataagtcctt catttttaga tttgtattct acatcagtgc attcaacaac
    20761 ttgtagagca tccatatcac ctctaagtgc tacagtcttt cctgaattag ccccttttat
    20821 agttgcaatt acacctgtac ctccagctga tacatatggt atccctattt tatcaagttc
    20881 ttctttaact ctctttgatg ttctaacttc ttccatactt ttctcaggat tttcatgaaa
    20941 ttcccttcta agcttaatta catagtcctt ataactcttt gttatttctt taatatccat
    21001 tcccgacctc caagtagtac acattcttct tatttaaaat actttttatc acttaaatac
    21061 tatattagat ttacaaagat acctgctatt actacagaag ctattgtaac tgtagtaaat
    21121 cctccaacaa gcatctttgg aagcatttta tccattaaaa attctttttc ttctggtgtt
    21181 tcagccaaag cctttactgc ctcttcagtt aatatatagt ttggtggaaa accataaagt
    21241 gatgtaagcg aaactgaaaa agccatttct ttactttcac ctaaaagctt acctactata
    21301 atagagaata ttcccatacc tattacacca aatattatga ttcctacaag aggaactgcc
    21361 atttcaacaa gcattgctgg cgtagctttt gctaaccctg caaatataaa agccatcaat
    21421 gatgtcatta accatccaaa tgaaccagat ttattaagtg gttttctatc aactattcca
    21481 agttctgatg ctattacacc aaatattaaa catataacat atcttgatac aacctcattt
    21541 gtaagatttg taaattgtac tgctaaccat gctacaaatc ctaatctaag tattatcata
    21601 tatgtcgtat catatttttc tggtaagtct ggaattatct taaatctgct tttttgttca
    21661 ggtaaatcct taactttttc ttcagctgta tttacagtta cttttccact tctaaagtct
    21721 gataaaagtc tttttccttc ttttttaagc attaatgctg taataggata acctgcaaat
    21781 ccttgtgcaa catacatcaa aattgcaagt actgacaaac ttgcaagacc ttttgctgta
    21841 gcagcatctg ccatcattat agatgcaact agtcctccag taagaggagg tgtagctatt
    21901 actgctgtat cccatccaaa tactactgtc cctaatgcca aagcacctac acatattcct
    21961 actattcctg ccaaggctat tactattgtt ttccattgac ctgctaattc cttaatactc
    22021 atcatagttc ccatatgagt tattaacaaa agcataccta attgagctaa tggtgttcct
    22081 aaagcagcta aatcaacaat attttctggg aaaaaagtcc aaaatcctac tataaatata
    22141 attgctgata caaaaaccga tggaacaaaa gctttagttt ttgctgaaac tatatctcct
    22201 atcgtcagcg atattaaaac tagacaaaaa gctgttattg cctccataca aaatcccccc
    22261 tcataataaa tgtattttta taaaatgtat gtatagttat ccattatttt actacttttg
    22321 aaaaacattt caagtcaaat ttcgattttt ttcataaaat tcgataaatt ccataaatta
    22381 aagttagttt tctaaattat tctttttgta aaaacgttat cctaagtatt ggctatttta
    22441 ctctattctt ataaatagaa ttaaatgtaa tttgcaaaaa ataaaacata ggatatattt
    22501 tattcataaa acacatcatc aatataaaat gaattgtctt tatgaatctt aatatatcct
    22561 atgtttgtaa gtttaatgtg tatgtttgct atggtgtaaa atttatatac tagttattgt
    22621 gtaacctact atatactcca tttttgttta ctaactcttc atgagttcct ctttcttcaa
    22681 ttccgttatc cgtcaatact atgatttcgt cagcattttt tatagtagtc aatctatgtg
    22741 caacaactaa atttgttcta tctttactta attcttctaa agactcttga atttctcttt
    22801 ctgttacatt atctaaggca gatgtagcct catccaatat tattattgga ggattcttta
    22861 aaaagattct tgaaattgaa attctttgtt tttgtccacc tgataatttt acgcctcttt
    22921 ctcctatata agtatcatat ccatctggca aagtctctac aaagtcatgt attctagctt
    22981 ttttagcagc agctatcatt tcttcatcag tagcattagg gttcccacaa agaatattat
    23041 ctcttatagt tcctgtaaat aaaaatacat cttgttgaac tatacctata ttttttctca
    23101 atgattttaa gctaacatct tttatactct ttccatcaat ttttatatca cccttatcaa
    23161 attcataaaa ccttggcaat agattacata atgtagtctt tcctcctcca gaaggtccaa
    23221 ctaatgctat agtcttacca gcttttatag ataaacttaa attatccaaa acttgagtct
    23281 tatcttcata tgtgaaagat atattttcta tctcaatgtc accttttaca ttttcaagtt
    23341 caataggatt tttagcttct ttttgatggt cttgttccat tatctctatg aatcttttaa
    23401 aacctgtcat accattttga tattgctctg taaagtttat taattttttc ataggttgta
    23461 taaacatttt tacatatagt atataagcag caaaatcacc caaagtaatc tttcctatat
    23521 aagtaaaata accaccagct attacaacta cccactctaa gatatccatg tataaattca
    23581 taccagagta atattctgcc attaccttat aagaatcttg tcttgctctt ctaaataatt
    23641 tattgcttct tacaaactta tttaactctt ctttctcagt acaaaaagac ttagttacct
    23701 tcatacctgc tatactattt tctaaagtag catttacatc acctatagta actctactct
    23761 tcataaaagc aatattcatc ttatcttttt tagctattgc aaaccaaatt ataaacggta
    23821 atatagcaaa tattataagc gtaagaggaa tatttatatc tataagtata aagaatgaac
    23881 caaccaacat tactatagaa atgaataaat cttcaggccc atgatgtgca agttctgtta
    23941 tatccattaa atcatttact attctagaca tagttactcc agatttgtta ttatcaaaat
    24001 acgtatttgg aagtctttgt aagtgtgtaa atacctcatt tctcatatca gcttgcatat
    24061 ctacaccaat gacatgtccc caatactgca taaaatagtt aagccctgct tttgctataa
    24121 atataagtat aagtgctact gcaaaaacta ctaacattct taagttttta tttggaacaa
    24181 cgtcatccat tatatttcta gttatcatag gataaactaa atcacataag gaaaatagaa
    24241 aagctgctat aaggtctagt gtaaaaattt tcttatatgg cttataatat ttgacaaatt
    24301 gttttatcat aagtattaaa tccctccttt attagtactt ctaaaaaata cccataaatt
    24361 agtataacat aacaataact ttaaatcttt tataaactta caaagaaata acaatctttt
    24421 tcataaaatt gaagatttat atcatatagt tgtatacttg aaacaacata aaaagagcta
    24481 tatctctata tttaaaattt tataaagata caactctata tttaatacta aatttttttt
    24541 ataaatacaa aattacttca atgcattcaa tatcttttta tctaatgctg ttgtatttat
    24601 acctgttata tatcctgttg cctttattgt aggtatatca taagttgttg gtagtatagt
    24661 agtagatacg attaaatcag caccttcttg ttttgttgaa cattcagcaa ttttacattg
    24721 ttcaacttct gcttgtatat tattttcttt aaccaatctt tctactctgt cacaaactat
    24781 tgttgatgtc gcgattcctg ctccacatgc tactaatatt tttttcatat attgtcctcc
    24841 tacaatttct ttaatatttt ataactcaaa ataaaattga taaatttctt atatctttat
    24901 ttata
//
LOCUS       DAEPXF010000043        24570 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain HB27
            SAMN13376389-rid12479423.denovo.045, whole genome shotgun sequence.
ACCESSION   DAEPXF010000043 DAEPXF010000000
VERSION     DAEPXF010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13376389
            Sequence Read Archive: SRR10518432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 24570)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24570)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001069624.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001069624.1
            Genome Coverage       :: 315x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/15/2021 01:54:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,165
            CDSs (total)                      :: 4,076
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 89
            rRNAs                             :: 10, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 10 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 19 of 69
            Pseudo Genes (incomplete)         :: 44 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 10 of 69
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24570
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13376389-rid12479423.denovo.045"
                     /strain="HB27"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="China: Hebei"
                     /collection_date="2017"
     regulatory      44..134
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            541..858
                     /gene="flgB"
                     /locus_tag="KRL67_001517"
     CDS             541..858
                     /gene="flgB"
                     /locus_tag="KRL67_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgB"
                     /protein_id="HBH1522405.1"
                     /translation="MNTYDIIKMGLDATNLRSQTIANNIANINTEGYKRHYITFEETL
                     NDTMPKIEEKTDNSKGLRVDGNNVDIEEEKVNQAMATLQYNALISFANGKIAMTKSII
                     SGR"
     gene            866..1270
                     /gene="flgC"
                     /locus_tag="KRL67_001518"
     CDS             866..1270
                     /gene="flgC"
                     /locus_tag="KRL67_001518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgC"
                     /protein_id="HBH1522406.1"
                     /translation="MSVFSGMRISASGLTAESLKMDIISSNVANINATRGPNGEAYRR
                     KVVSFEENYDNKLGMLGVKVTSVEDDKSPLKEVYDPSHPDAKPNGYVEYPNVNILVEM
                     TDLITASRAYDSNVDTLNAQKSMISKALEIGK"
     gene            1280..1597
                     /gene="fliE"
                     /locus_tag="KRL67_001519"
     CDS             1280..1597
                     /gene="fliE"
                     /locus_tag="KRL67_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein FliE"
                     /protein_id="HBH1522407.1"
                     /translation="MPITQINNLMQGAKLDNLIGEAKPLTLNNKEEKKNFEDVIIDTI
                     DKVNFQQITADDMKEKFIKGEDVSMHEVMLIGQEAQLSMQYLIEVRNKIHDAYQEMNK
                     VQI"
     gene            1622..3172
                     /gene="fliF"
                     /locus_tag="KRL67_001520"
     CDS             1622..3172
                     /gene="fliF"
                     /locus_tag="KRL67_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar M-ring protein FliF"
                     /protein_id="HBH1522408.1"
                     /translation="MITSFKNKIKEQVAKSRTKISSLKKGTKIALGIGVIAICLALVF
                     TAISASKNKYAVLFSELDAQDAADISAELESKKIDTKVKGSTIYVPRTEVDKLRLELA
                     PSLTGGSKGFALMDDSSSIGLTDEEFQIKKQRMIQGEIEKTIKSFPQVEDTRVHITFG
                     ESSVFEKEAVPGSAAVYLMLKPGTTLEKNQIKSVMALISGSVTNIPEKNVQVIDNKMN
                     LLSEGILEDENVDENNQPINSGISVDKNKASEQALNKQLEKSILEMLEPIFGKGKVKA
                     TVNADLSFDTAEKTEIKIDPDKVAIKETRSKNSSKSATNNVQGVDANMNNQGNNNGTN
                     TEDSLDESFEYETGKIETHTIVAPGELNRLTASVAIDGKIAKGVQADVEDIVNNAIGM
                     DGARGDTLSVVSMVFDPEGKKEAKQELEEIKKEGFKKNIIKAVIGVVAVIAVGAIGYL
                     IYKKRSEQEDMDYEDEDYISKSDSVDRKDNPLLGDDPELTLEEAVKMYAEEKPDQVTE
                     IIKTWLNE"
     gene            3185..4255
                     /gene="fliG"
                     /locus_tag="KRL67_001521"
     CDS             3185..4255
                     /gene="fliG"
                     /locus_tag="KRL67_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliG"
                     /protein_id="HBH1522409.1"
                     /translation="MSTEITNVNAFDLSDISESDKNEKIEGEGLKKAAVLLMTLGPDV
                     SSDIIKSLPDKQVQKIGLEIANISSIASKERREILKEFVEINRAKDYVIEGGLEYARE
                     LLSNALGNAKANKLLEGISINTSTKPFKHIIKLDVKKILEILRNESAQTIAVLLAHME
                     PENGAEVLSYLDFDLQNEVAMRISSMTSVSPEVIKVMESSLEDKLHSISGDDFRDYGG
                     VSTLVQILINSDRKTEKNILGTIEEQDPALAAQIKSHIFIFEDIVKLDDISIQKVLKE
                     VTMKDLVYALKGVDDAISDAIYRNQSSRAADALREEMEMVGVIKVTQVEAAQQKIVNS
                     VRKLEREGAITIVRNFGDDFVE"
     gene            4239..4979
                     /locus_tag="KRL67_001522"
     CDS             4239..4979
                     /locus_tag="KRL67_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly protein FliH"
                     /protein_id="HBH1522410.1"
                     /translation="MTLLNNKIIKSKKASYKGVLDLSIIDKVRVGESNLNKENDCRFR
                     DEVIKEAIESAEIKKNEILEEAKMNAQNIEKQAYEKGYSQGKKNGYEDGYKESYDEYT
                     QKAKDEADFIKNQANLILLQANEKMTEYIKEKNKEIVRLAINMASSVLKKHFEDEESM
                     SDLLKQIIQDYEGSSSFIVRCNSFYKDNIEKEFKLFKEETSLKSSVFVIADDSIDKGN
                     AVIQKENGRIIVGIDCAMEKLKEELLGE"
     gene            4983..6302
                     /gene="fliI"
                     /locus_tag="KRL67_001523"
     CDS             4983..6302
                     /gene="fliI"
                     /locus_tag="KRL67_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012705235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein export ATPase FliI"
                     /protein_id="HBH1522411.1"
                     /translation="MYKTDIYMDFDKINKKIENLELTISEGRVKKIIGLTVEVEGIKA
                     FVGELCVIYNQVNKPVNCEVVGFKDKEVILMALGELTLIAPGCKVISKGIPLSVMCSD
                     NLLGKVLDGLGNPIDNSDAVLGDRYNLNNEPPDPMKRKKIRNIMETGVRAIDAFTTCG
                     EGQRIGIFAGSGVGKSTTLGMIARNAKADVNVIALIGERGREVLDFIDKDLGEEGMKK
                     SVVVCATSDKAPLVRLKGALTATAIAEYFRDQGKKVILMMDSVTRFAMAQREVGIAIG
                     EPPAQKGYTPSVFAILPKLMERTGTSDKGSITAFYTVLVDGDDFNEPIADTTRGILDG
                     HIVLSRDLANKNHYPSIDVLNSLSRLMNEIASKEDIKIASFARDMLAEYREAEDLINI
                     GAYASGTNKKIDEAIYYHEQIINFLKQGINEKSSFNETTSSLKRIFE"
     gene            6334..6780
                     /gene="fliJ"
                     /locus_tag="KRL67_001524"
     CDS             6334..6780
                     /gene="fliJ"
                     /locus_tag="KRL67_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012815984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export protein FliJ"
                     /protein_id="HBH1522412.1"
                     /translation="MELDKNFKFRLQKVLDLKMKDEEKIKMEFAKIQQKKIDIETNLE
                     NLESNYSKYSISKNNDSIQEQKITINYLLALNNSIMDLSEELDKSTNELEKARKQLID
                     KQIERKSLEKLKEKKYGQYYKEENLKEQNTNDEFASMSYLRNRQVL"
     gene            6809..8014
                     /locus_tag="KRL67_001525"
     CDS             6809..8014
                     /locus_tag="KRL67_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-length control protein FliK"
                     /protein_id="HBH1522413.1"
                     /translation="MNIQTDSYNVIKDIGDKKSSSKSKNVKNNKFEDNLYKATDNKQV
                     KGRKEMSNQNINSNKEENSENECHLDEDKSIHLEDDSTYEKDFSKNNEILQQLLNLLK
                     NNDKKDKDINSILNDLEKLNISDELKQELKLNKNVLNKIVNQNAKLNNANINNSDDMQ
                     SLDMMKLRELLDNDANKNFEVISKTTENDTKSIDLYNFDSNRMDNINSNKQKDDSSDI
                     LEKLAGGNGNKSGNFGQVINKNLDTNKVKNDNVNLKHIESNVMDDSIKAIKHMKTNDI
                     QELTIKLRPKELGDMNIQLLKDGENMRAVVTVFNKDVFDSINKNMTDLKQHLELTNVN
                     IKDVSVQMHSDNRNTSDTFDKAFEQQNRQNNQENMNKQNTNNKRNNMAIEDEIKENII
                     DDDRVDLLA"
     gene            8032..8652
                     /locus_tag="KRL67_001526"
     CDS             8032..8652
                     /locus_tag="KRL67_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlgD"
                     /protein_id="HBH1522414.1"
                     /translation="MYGVATALAGAQGSYRSNNLSQLERNTNDIANKQVRSKGNQTLT
                     DNGTPIIGQGDDADKDLFMKILVAQMGNQDPMNPQDPTEYVSQLAQFATLEKMSAMNE
                     KLDILLALSNSTLINSALSMATSLIGKKVEVSGEEKEDENIVGAVKSTYIKDGEVHLE
                     LKVEGSDKTVDVKYEKLLKIGEVDTNSDEEENSPESGEEESSTEQV"
     gene            8679..9665
                     /locus_tag="KRL67_001527"
     CDS             8679..9665
                     /locus_tag="KRL67_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein"
                     /protein_id="HBH1522415.1"
                     /translation="MIKAMYSGVSGMKANQTKLDVIGNNVANAGTTSFKKSSARITDS
                     FYQTVLYASAPTAALGGTNLGQVGVGSKISSINKDMTQGNVQPTNRPSDLMIDGDGYL
                     PVVRQGTVMYTRDGSFNLDTGFKAAAGPLKDVKGGRLVNGDGYMLQGMIYEGEYKADT
                     GEFVKKGDAKGKEPIVIPLQSISPKDGTVQNVLEYNISKDGTIEFLLSDGQRTQFVYN
                     NIPDDAKVGDTPAGIDKLKGTIQKVQTYAFQNPAGLDAEGGNLFKPSANSGDPQIAGA
                     TGTIIQGAIEASNVDIAEEFTEMIIASRSFQANSKIISTSDEILQEIINLKR"
     gene            9678..9881
                     /locus_tag="KRL67_001528"
     CDS             9678..9881
                     /locus_tag="KRL67_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar FlbD family protein"
                     /protein_id="HBH1522416.1"
                     /translation="MIRVTDLNGRKYILNSDLIVRIDSVPESRILLTTGEKNLVKESV
                     DEIVDKVIKYKRKINVGYIRSEK"
     gene            9881..10699
                     /locus_tag="KRL67_001529"
     CDS             9881..10699
                     /locus_tag="KRL67_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HBH1522417.1"
                     /translation="MKKTDSLTFIGLIVSTVLVLVGAAKGSSSGLKNFFDVSSILITV
                     LGSFGALMITFTIDDIKLIKNALQYSFKTMSVSKLDLLEQFKTLSKKARKEGLLSIES
                     DVMGIEDPFMKKGLELCIDGLEINEIRSILELEIDSVEDTNNRYSKLFKTWGTFAPAF
                     GMLGTLIGLIQMLADLTSPDLIASGMGKALITTFYGSLLANIALNPIAYNIDEKTEKE
                     IYVKEMMLEGIISIQSGESSIVVEERLATYLSNNEKLEIMKSNKNTERAMSNGA"
     gene            10689..11384
                     /locus_tag="KRL67_001530"
     CDS             10689..11384
                     /locus_tag="KRL67_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HBH1522418.1"
                     /translation="MARKKNKKKDEINPDAWLATYADTITLILTFFVLLYATSVVDME
                     KFKNIADALKGQFSGTSIFSTGSSTSEKSPIDSVLVDKNDKSNLEQDLKDKVNNSNLQ
                     NSVTVKDDSRGILLELDDSILFDSGISELKVDSKATLNKVYDMIKMMKNKIVIEGHTD
                     NVPVKSSTHESNWDLSSSRAVSVVRYFVEEKGMDPKLFSATGCGEYQPLVSNETAKGR
                     AENRRVNILITID"
     gene            11396..11839
                     /locus_tag="KRL67_001531"
     CDS             11396..11839
                     /locus_tag="KRL67_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body-associated FliL family
                     protein"
                     /protein_id="HBH1522419.1"
                     /translation="MFKNKKIIIIILILIIFSAGAGFFILGEGGNKQIDKIAGLFIPT
                     GKGKDEVKTENLDLEEMVLKLADEGVRYLKVSITVSYDSSYKDIEKNTPLIRDNIINC
                     FMAKRADDFTAENLNNIKEEIKNTLNIQLGENVIQNIYFTNIVVQ"
     gene            11854..12228
                     /locus_tag="KRL67_001532"
     CDS             11854..12228
                     /locus_tag="KRL67_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliZ"
                     /protein_id="HBH1522420.1"
                     /translation="MILYLLKILICIPIILILIVLSLKISQGTLLKKYNDKYVKILDV
                     VNISKNNSIIILKIGEEGCIMASSSSSINKIKDLSKEEILKIENDIKERNFKIQDFKM
                     ERIKKKYDFSRYIGKFTKKEDK"
     gene            12229..12894
                     /gene="fliP"
                     /locus_tag="KRL67_001533"
     CDS             12229..12894
                     /gene="fliP"
                     /locus_tag="KRL67_001533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425215.1"
                     /note="The bacterial flagellar biogenesis protein FliP
                     forms a type III secretion system (T3SS)-type pore
                     required for flagellar assembly; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system pore protein
                     FliP"
                     /protein_id="HBH1522421.1"
                     /translation="MELLSKLPLADVPYTSSMQLFLFLTALMFLPFVMFMMTSFVRIV
                     ISLSFLKSALGAQQAIPSQILVGLAIVLTIFIMRPVLNEINEKALQPYMKEEVTMEEA
                     MKEAEGPIKEFLLKQTRQTDLDLFVEQAGLKEKKLTRENIPLSVVVPAFAISELKTAF
                     QIGFLIYIPFLIIDLVVASVLMSMGMFMLPPVMISLPFKLLLFVMVDGWNLIVKTLIL
                     GFG"
     gene            12907..13176
                     /gene="fliQ"
                     /locus_tag="KRL67_001534"
     CDS             12907..13176
                     /gene="fliQ"
                     /locus_tag="KRL67_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliQ"
                     /protein_id="HBH1522422.1"
                     /translation="MSEFMIMSVLKNGMFTGAMIAAPVLIVSLVVGLLISVFQATTQI
                     QEQTLTFVPKLIAILLVVAIGGSWMLHTLISFTSEIFNMVSLITK"
     gene            13188..15002
                     /locus_tag="KRL67_001535"
     CDS             13188..15002
                     /locus_tag="KRL67_001535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused FliR family export protein/FlhB family
                     type III secretion system protein"
                     /protein_id="HBH1522423.1"
                     /translation="MIFVFIRIVAFFGTLRILFPAGTPALFKSLFAIIISILISSTMQ
                     IEYATNIDNIVLFTLYGINETITGIMLGYITNLCFYSIRMAGSMMDQQMGLSMINMFD
                     PNSLTQTTLIDNLMNWTALMIFFSMDGHHVLIRGIRYSFELIPIGKSFIDNNIDYIIN
                     IFVQCFLTGFKIAIPIVLCLLMADFILGLISRSVPQLNVMIVGMPLKILVGIALFIIS
                     IPLIVNQISHLLSQIPKMYEGTFALAPMFFMGSADKTEEATPKKKGEQRKKGNIAKSR
                     ELPVAMTLLAFTLLVPTLFSYVVDTLKSSLNYFLSLDFYMNINYSNLEKLVIAGLIDF
                     FKIFLPIAIPFLVLGIIANLFQVGILFTGETLKPNLSKLNPVNGFKNMFSMRSLSTLI
                     KDTAIISILAYIGYTFFQDNYLDILKLGNIYLPTLMYTVKDLVYSILSKICVAMIAIA
                     VADYVYQRYSHKKQLRMTKQEVKDEYKNSEGDPEVKAKIKQKQRQISSQRTMQAVPSA
                     TVIVTNPTHLSIAVRYEKGKDQAPVVVAKGADYLAFKIREIAKGNDIPIIENKPIARL
                     LYKQVEIDQEIPEDMYQAFAEILVAVYKIKNRYKVPKR"
     gene            15011..17086
                     /gene="flhA"
                     /locus_tag="KRL67_001536"
     CDS             15011..17086
                     /gene="flhA"
                     /locus_tag="KRL67_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
                     /protein_id="HBH1522424.1"
                     /translation="MNKFNLKENMDVIVAFGVVGIVLMMIVPLPTFILDILLAINISL
                     SVLILLMTLFTTNVLDLSIFPTVLLVTTLFRLGLNLSSTRLILTQGYAGEVIEAFGSF
                     VVGGNYVVGIIIFLIILIVQFVVITNGSGRVSEVTARFTLDAMPGKQMSIDADLNAGV
                     IDDKTARKRRKELQQEADFYGSMDGANKFVKGDAIAGLIVTAINIIGGIVIGAVMLNM
                     TLMGAAQTYVRLTIGDGLVSQIPALLISTSAGIIVTRASTDENFGGLVGKQLTAIPKA
                     VMMTGCVLIVLGMMPGLPKPAFFSLGIGAIAAGYFLGREKKDTLEDLEFEKPDMVNPD
                     KNEVEDVTNLINVESIEVEIGYGLISLADESAGGDLLQRIASIRRQCAIDMGIVVQPI
                     RIRDNLQLNPNQYNIKIKGNVVGGYEIMPNMVLCMDPMNQGFSIFGIKTIEPTFGIEA
                     LWIESDKVEDAELKGYTVVDSSTILITHLLDIIKSNAHELLGRQEVKTIIDAARENYS
                     AVVDELIPDLMTLGEIQKVLKNLLREKVNIKDRVTILETLADQSRNTKDIELLTEYVR
                     IALARSICNNLVDDDKTIVVATLSPETEELVSNNLQRSVNGTYPAVDPENTNKIFESI
                     QEVMSNVYFNHNIPVIVVSPKIRAPFRKLVEIVYPNLTVLSLNEIPNDIKIKAEGVVS
                     IYDDEKVYS"
     gene            17061..18077
                     /gene="flhF"
                     /locus_tag="KRL67_001537"
     CDS             17061..18077
                     /gene="flhF"
                     /locus_tag="KRL67_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhF"
                     /protein_id="HBH1522425.1"
                     /translation="MMTKKYTANTIQDAMNLAKMELGDNITLIDKKEVRKSGIQGIFL
                     KKDIELTIGWEKRENVEQKDLKREIEQLKSIINNMGFDNKNDIDIDKICKNLLSLELN
                     EEIVESIRIDLQEMKFNGIDTSKNLVEILKKKIKIENQAINGKIALVGPPGVGKTTTI
                     AKLAAKLVFEENKKVGVITIDTYRIGAVEQLKIYTDIMNIPFKGVISPDEMELALDEM
                     KDCDVVLIDTTGRGYKNSMQILEIKNLIDKAETDNIHLVLNCTTRESDTKAIIDSYRN
                     VNFKSLIITKLDETITYGSIFNIVNYAQKPISYITTGQNVPDDIIKPNEDKIIRLLLG
                     VESI"
     gene            18074..18952
                     /locus_tag="KRL67_001538"
     CDS             18074..18952
                     /locus_tag="KRL67_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MinD/ParA family protein"
                     /protein_id="HBH1522426.1"
                     /translation="MIDQAQILRKMAMEKRGSDEIIVENENTPKIITIASGKGGVGKS
                     NLATNLSICLTKLNKKVLILDADIGMSNIDIIMGVNVKGTIIDVINGEKNIEDIISQT
                     KYGVNIISGGSALNHIEDFTEAQRNKFIHSIEQIHDVDFIIIDTGAGMSKSLLSFIYC
                     STEFFLITTPEPTSLTDAYSLLKAISNFGIKRSANIIINRVIDLEEAKSTYKRIKMVV
                     DKFLNLNLELYGYIVDDKKVGLCVKKQIPFILEYPTSIASKCIIAIAKRMVSQSREDI
                     VDNKSFLRKMFSIFKS"
     gene            18966..19667
                     /locus_tag="KRL67_001539"
     CDS             18966..19667
                     /locus_tag="KRL67_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliA/WhiG family RNA polymerase sigma factor"
                     /protein_id="HBH1522427.1"
                     /translation="MNREELIKENMPLVKSIARKFFIPGKGFEYQDLVNSGVIGLIDA
                     INKFDVEKGAKFSSYSYIKIKSAILDEIRNQSPISKHNLTKVNKYNRVVEKLQSKLLR
                     EPTSYEIAKELKVSEKELHDIESNIDMLNIVSLNYVIFEDTNETVQDVISDREEEAPE
                     NIIEEEEKLEILSKAISNLNEREKLILSLYYYEDLNLKEIGKVLGVSESRVSQLHRKS
                     IRNLRNKIKELKYSI"
     gene            19683..20105
                     /locus_tag="KRL67_001540"
     CDS             19683..20105
                     /locus_tag="KRL67_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein"
                     /protein_id="HBH1522428.1"
                     /translation="MIQIILIVVSILIIVTIVINLHKESKNKDNRYFDKIFEQEYEAN
                     DRLIIAEKRRFFLDKVLAEIRSNNHIKVYENYNLIKANEERKESIFIESKKEVRQENE
                     LSLKVKHLISSGYDDVEICKMLDIGKGELSLIRSCYKI"
     gene            20090..20275
                     /locus_tag="KRL67_001541"
     CDS             20090..20275
                     /locus_tag="KRL67_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein"
                     /protein_id="HBH1522429.1"
                     /translation="MLQNIRILLCDKKVFLGIGIGILISTLCIMLFSNNDMSKAEIEK
                     KARALGMKYTSEMKVLE"
     gene            20316..21089
                     /gene="flgG"
                     /locus_tag="KRL67_001542"
     CDS             20316..21089
                     /gene="flgG"
                     /locus_tag="KRL67_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgG"
                     /protein_id="HBH1522430.1"
                     /translation="MIRGLYTAVSAMITNQQKQNVVVNNLANMDTNGYKSKQLLTKSF
                     DELKLEGYNNYANGTKQKQVIGDISPGISMDETITNFNQGPIKTTDNKMDVAIQGKGF
                     FLVSDAAGNQFYTRNGNFRTDNQGDLITSEGYYVLGTNTATGAVGHINVGNQKFEVAK
                     DNTISLDGNAMYKFNIVDFNNYNNLKKEGDNLYSGGGAMKVNNALTIQSAIESSNVDM
                     VSEINNMMTISREYEANQKIIQAMDSKLAKIASEIGSVR"
     gene            21110..21871
                     /gene="flgG"
                     /locus_tag="KRL67_001543"
     CDS             21110..21871
                     /gene="flgG"
                     /locus_tag="KRL67_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgG"
                     /protein_id="HBH1522431.1"
                     /translation="MYTMFYTSKTALSANQSKLDIISNNIANSDSTGYKKINMEYSDL
                     VDEALNRPSYPTNGNDISTGTGAKATAPMRIYEQGALAPTDSKSNLAIDGEGFFRVIR
                     KDGTYAYTRNGGFNVDALGKIVDDNGNILDVQFEAGYNYNNTNIDSTNLTVSRNGELF
                     VDNKKIGKINLYQPIGTQNFISEGDSLFVATDAAQIKQVEKVDIVQGYRERSNVSLQE
                     EFVDLIATQRAFQMNSKGIKTADEMWQIANNLRAK"
     gene            21894..22775
                     /locus_tag="KRL67_001544"
     CDS             21894..22775
                     /locus_tag="KRL67_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliM/FliN family flagellar motor switch protein"
                     /protein_id="HBH1522432.1"
                     /translation="MNDMKEYDFGKPQSYSRENLTCLNFLLAEFCKKYKNYIIYELKC
                     NSSMVVDSIDQVNYQTFLDRVTSSCVVVQNAMEPVMKNFSFRIDRSVADMWIDITSGG
                     TGVVRDNDRELTELDKKVLLHLMDDLVRNMHLFEGFENIQVLDTHTHINLPQLCSPTA
                     PVCVVDLKVLNDNEYIGSVSLCFPYNGLEALLESISVIEFFDNDVGDDSEEFTKKIYN
                     SVSNIDLSVIAEIGKVSINVEDLLKLEVGDVIVTNKKINDYIDIFVENSKSYTATPGF
                     ISSKKGVKINDAVGKEV"
     gene            22778..23152
                     /locus_tag="KRL67_001545"
     CDS             22778..23152
                     /locus_tag="KRL67_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliM/FliN family flagellar motor switch protein"
                     /protein_id="HBH1522433.1"
                     /translation="MDNSNLISDEEAKMLLNDKVESEVDGDDELALDVSEVNRNNIQR
                     ILDLKLELSVVIGRTKMTLRDILNLHKGSLIELDTLADQDVEILIDNKVLAYGKVVVV
                     DLNFGVKITSIVSEEDMVKSLV"
     gene            23184..>24570
                     /locus_tag="KRL67_001546"
     CDS             23184..>24570
                     /locus_tag="KRL67_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HBH1522434.1"
                     /translation="MVLILNLNDIKKDILKFNKKDSCSLYNESINNINNNCISTGLKF
                     LKQARDLNPDDVDILNLTGLVNLLKCNFDDAVESFYKSSLCEKTALCEKYINMLTSEE
                     FLIFFEKYNQLIRLINKGMYKEAIQGFKLITDQYCDLIEPYELLALLYDKEEEYIKFD
                     ECLEVLKIIDKENYLLNQENNILDMYNSANIQTKSNIKVDDYKNEFLHKSSNNHKIND
                     YNNVTKEKSSSKNQKSSDSNKKKSIKKNKYLYIVIILGILLVGQSIYSSYKMNNLTDK
                     ISKNNETKNEGSIDSSLDKKENNIKENEDMNKTSNINKSNKNINKEDVKSKKNTVENE
                     DDKESDLFTEDELMSKANTLKSEKKTKSSIQLYKKVADIGENKGNTSEATYQVAILSE
                     KLKDYKTAEEYYKMYVENYSEKDAYFDESYYNLGMMYYNNGDLKSSKLTLKKLVNKVP
                     NSMYNNSKVKEI"
BASE COUNT        10134 a         2640 c         4613 g         7183 t
ORIGIN      
        1 aaaaatattt aaaaaatttg ttatttattg tatacttaag ttaaaactaa ataggcaaat
       61 ctagagaaat ctagtgacgc aaagctatag ggactaaggt ttgtatacat gaactatgtc
      121 agccagttgc caaaaagagt cctaggtgta ttgtatacct aagaaaagtc tagaatgact
      181 gcctttttgg cagtcatttt gttatggaaa aattacttta ttttagtaga aaacacatat
      241 aataacatat tttaccaatt ataacataaa ataaagaaaa attatctcaa aaagttacta
      301 ttttacaaaa aatgacttct ttattcattt aaattaatcg catttccatt acattaatac
      361 aataatgtat cattttagta agaaaactaa tattttttca atgaagtccc atgaaaatat
      421 aaaaaaattt taatatgtat ttaaatattt cttcaaaaag ttgctctttt ttgtaatata
      481 gcaacttcat tttttattaa taaacaaaat agataaaata tattttgagg aggggtgagt
      541 gtgaatacat atgacattat aaaaatggga ttagatgcaa ctaatctaag aagtcagaca
      601 atagcaaata atatagcaaa tataaatact gaaggatata aaagacatta tataactttt
      661 gaagaaacac taaatgatac tatgcctaaa atagaagaaa aaacagacaa ttctaaaggt
      721 ttaagagtag atggtaataa tgtagacata gaagaagaaa aagtaaatca agcaatggca
      781 acattacaat ataatgcttt gataagtttt gcaaatggaa aaatagcaat gaccaaatca
      841 ataataagtg ggaggtaatt agaagatgag tgtgttcagt ggaatgagaa tatctgctag
      901 tggattaaca gcagaaagtt tgaaaatgga tatcatatca tccaatgtag ctaatataaa
      961 tgctacaaga ggtccaaatg gagaagcata taggagaaaa gttgtttcat ttgaagaaaa
     1021 ctatgataat aaattaggta tgctaggagt aaaagttaca tctgttgaag atgataaatc
     1081 acctcttaaa gaagtatatg atccatctca tccagatgca aagccaaatg gatatgtaga
     1141 atatccaaat gtaaatatat tggtagaaat gacagattta ataacagctt cacgagcata
     1201 tgattctaac gtcgatacat tgaatgctca gaagtctatg atatcaaaag ctttggaaat
     1261 aggaaagtag ggataaatta tgccaattac acaaattaac aatttaatgc aaggtgcaaa
     1321 gttagataat ttgattggag aagcaaagcc attaactctt aataataaag aagaaaaaaa
     1381 gaattttgaa gatgtaataa tagatactat agataaagtt aattttcaac aaataacagc
     1441 agatgatatg aaagaaaaat ttataaaagg cgaagatgtt tctatgcatg aagttatgtt
     1501 aataggtcaa gaagcacaac tatctatgca atatttaata gaagttagaa ataagattca
     1561 tgatgcatat caagaaatga ataaggtgca aatttagcct tttgaacagg agtaataatc
     1621 aatgataacc agttttaaaa ataagattaa ggagcaagtt gctaaatcta ggacaaagat
     1681 aagttcttta aaaaagggaa ctaaaatagc attaggtata ggggttattg ctatatgttt
     1741 agcgctagta tttacagcaa tatctgcgag caaaaataag tatgcagtct tattttcaga
     1801 actagatgct caagatgctg cagatatatc agcagagcta gagtctaaaa agatagatac
     1861 taaagtaaaa ggaagtacga tttatgtacc tagaacagaa gttgataagt taaggctgga
     1921 gttagcacca agtcttacag gtggttctaa aggatttgca ctaatggatg atagttcatc
     1981 aataggactt actgatgaag aatttcagat taagaagcaa agaatgatac aaggtgaaat
     2041 agaaaaaaca ataaagagtt ttccacaagt ggaagacact agagtacata taacatttgg
     2101 agagtcatca gtatttgaga aggaggctgt tccagggagt gcagcagttt atttgatgct
     2161 aaaaccaggg acgactttag aaaaaaacca aataaagtct gttatggcac ttatatcagg
     2221 aagcgttaca aatataccag agaaaaatgt tcaggttatt gataacaaaa tgaatttatt
     2281 atctgaaggt attttggagg atgaaaatgt tgatgaaaat aatcaaccaa taaatagtgg
     2341 cattagtgtt gataaaaata aggcatctga acaagcattg aataaacaac tagaaaagtc
     2401 tattttagaa atgctagaac cgatatttgg taagggaaag gtgaaagcta cagtaaatgc
     2461 tgatttaagt tttgatacag ctgaaaaaac tgagataaaa attgaccctg ataaagttgc
     2521 aataaaggag acaaggtcga aaaattcatc taaatctgct acaaataatg ttcaaggtgt
     2581 agatgctaac atgaataacc aaggaaataa caatggtact aatacagaag atagtttaga
     2641 tgagagtttt gagtatgaaa caggtaagat tgaaactcac actatagttg cgcctggaga
     2701 acttaataga ttgacagctt ctgttgcaat agatggaaaa atagctaaag gtgttcaagc
     2761 tgatgtagag gatattgtta ataatgctat aggtatggat ggagcgaggg gagatactct
     2821 tagcgttgtt tcaatggtat ttgacccaga agggaagaaa gaggctaaac aagagctgga
     2881 agagattaaa aaagaaggat ttaaaaagaa tataataaaa gcagttattg gagtagtagc
     2941 agtaatagca gttggagcta ttggatactt aatatataag aaaagaagtg aacaagaaga
     3001 tatggattat gaagatgaag attatatttc taaatctgat tctgttgata ggaaagataa
     3061 tcctctatta ggagatgacc cagaactgac attagaggaa gctgttaaaa tgtatgcaga
     3121 agaaaagcca gaccaagtaa cagagataat aaaaacttgg ttaaatgagt aagggggata
     3181 gaaagtgtcc acggaaatta caaatgttaa tgcgtttgac ttaagtgata taagtgaaag
     3241 tgataagaac gagaaaatag aaggtgaagg tttaaaaaaa gctgctgtat tattaatgac
     3301 attaggccca gatgtctcta gcgatataat aaaaagtttg cctgataaac aagttcaaaa
     3361 aataggtttg gaaattgcaa atatttcttc aatagcttct aaagaaagaa gagaaatctt
     3421 aaaagagttt gtagaaataa atagagcaaa agattatgtt atagaaggcg ggcttgaata
     3481 tgcaagagag ttattaagta atgctttggg aaatgctaaa gcaaacaaat tacttgaagg
     3541 tatatcaata aatacaagta caaagccttt taaacatatt atcaaactag atgtaaagaa
     3601 gatattagaa atacttagga atgaaagtgc acaaactata gctgttttac ttgcacatat
     3661 ggaaccagaa aatggggcag aagttttatc atatttagac tttgatttac aaaatgaagt
     3721 agctatgaga ataagttcaa tgacttcagt atctccagaa gtgataaagg taatggaaag
     3781 ttcattagaa gataaattac attcaataag tggtgatgat tttagggatt atggcggagt
     3841 gtctacctta gttcaaatat tgattaactc tgatagaaaa acagaaaaga atattttggg
     3901 aacgattgaa gaacaagacc cagctttagc agctcaaatt aaatctcata tatttatctt
     3961 tgaagatatt gtcaagctag atgatataag tatacaaaaa gtgttaaaag aagtaactat
     4021 gaaagattta gtatatgcct taaaaggtgt ggatgatgcg atttcagatg ctatatatag
     4081 aaatcaatct tctagggcag cagatgcact tagagaagaa atggaaatgg ttggtgttat
     4141 aaaggttaca caggttgagg ctgctcaaca aaaaatagta aatagtgtaa gaaaacttga
     4201 aagagaaggc gccataacta ttgtaaggaa ttttggtgat gactttgttg aataataaga
     4261 ttattaaaag taaaaaagca tcatataaag gagttttaga tttatccata atagataaag
     4321 ttagagtggg agaatcaaat ctaaataaag aaaatgattg tagatttagg gatgaagtaa
     4381 taaaggaagc tatagaatct gcagagataa aaaagaatga gatattagaa gaagcaaaaa
     4441 tgaacgcaca aaatatagaa aagcaagcat atgaaaaagg gtatagtcaa ggtaaaaaaa
     4501 atggatatga ggatggatat aaagagtcat atgatgaata tacccaaaaa gctaaggatg
     4561 aagcagattt tataaaaaat caggcaaatt taatcttatt acaagctaat gagaagatga
     4621 ctgagtacat aaaagaaaaa aataaagaga tagtaagact tgctattaat atggccagtt
     4681 ctgttttaaa aaagcatttc gaagatgaag aatctatgag tgatttgtta aagcaaatta
     4741 ttcaagatta tgaaggaagt agttcattta tagtaagatg taattctttc tataaagata
     4801 atatagagaa agaatttaaa ttatttaaag aggaaacatc actaaaatca agtgtatttg
     4861 ttattgcaga tgatagtata gataaaggaa atgctgttat ccaaaaggaa aatggaagaa
     4921 taatagttgg tattgattgt gctatggaaa aattaaaaga agaattgctt ggtgaataaa
     4981 gtatgtataa aactgatata tatatggatt ttgacaaaat aaataagaaa atagaaaatt
     5041 tagaactcac tatatcagaa ggtagagtaa agaagataat aggacttact gtagaagttg
     5101 aaggaataaa agcttttgtt ggggagttat gcgttatata caaccaagtt aataaaccag
     5161 tgaattgtga agtagtaggt tttaaagata aagaagtcat attaatggcg ttgggagagt
     5221 taacattgat agctcctggt tgtaaagtta tttctaaggg gataccttta agtgttatgt
     5281 gtagtgataa tttattaggt aaagtacttg atggtcttgg aaatcctata gacaactcag
     5341 atgctgtttt aggagataga tacaacttga acaatgaacc tcctgaccca atgaagagaa
     5401 aaaagatacg aaatataatg gaaacaggtg taagagctat agatgcattt actacttgtg
     5461 gtgaaggaca gagaatcggc atatttgcag gaagtggagt aggtaagagt acgactctag
     5521 gtatgattgc taggaatgca aaagcagatg taaatgtaat tgctcttatt ggagaaagag
     5581 gaagagaagt actagatttt attgataaag atttaggtga agagggaatg aaaaaatctg
     5641 ttgttgtgtg tgcaacatca gataaagccc ctctggttag gctaaaaggt gctcttactg
     5701 ctactgcaat agcagagtat tttagagacc aaggcaagaa agtaatactt atgatggatt
     5761 cagtaactag atttgctatg gcacaaagag aagttggtat agcaatagga gagccaccag
     5821 cccaaaaagg atatacacca tctgtatttg ccattttgcc aaaacttatg gagagaacag
     5881 ggacatcaga taaaggttct ataactgcat tttatacagt attagttgat ggagatgact
     5941 ttaatgagcc aatagcagat acaacaagag ggatacttga tggtcatata gttttatcta
     6001 gagacttggc aaataagaat cactatcctt ctatagatgt attgaacagt ttaagtagac
     6061 ttatgaatga gattgcttca aaagaagaca taaaaatagc ttcttttgca agagacatgc
     6121 ttgctgagta tagagaagca gaagatttga taaatatagg tgcatatgct tcaggtacaa
     6181 ataaaaaaat agatgaagca atctattatc atgaacagat tattaatttt ttgaaacaag
     6241 ggataaatga aaagagttct tttaatgaaa ctacaagtag tttgaaaaga atatttgaat
     6301 aaataattta aaaaaatatt tgaataggtt gtgatggaat tggataaaaa ctttaagttt
     6361 agattacaga aagttttaga cttaaaaatg aaggatgaag aaaagataaa aatggaattt
     6421 gctaagattc aacaaaaaaa aatagatata gagactaact tagaaaattt agaaagtaat
     6481 tatagcaaat attctataag taaaaataac gatagtattc aagaacaaaa aataactatt
     6541 aattatttgt tagctttaaa caactctata atggatttat cagaggaact ggacaagtca
     6601 acaaatgaac ttgagaaagc tagaaaacag cttatagaca aacagataga aagaaaatca
     6661 ttagagaagt taaaagaaaa aaaatatggg cagtattata aagaagaaaa tttaaaagaa
     6721 caaaacacta atgatgagtt tgcaagtatg agttatttaa ggaatagaca agttctataa
     6781 gaatagtagg aagtgaggat attattgtat gaatatacaa acagattctt ataatgttat
     6841 taaggacatt ggggataaaa agagttcatc taagagtaaa aacgtaaaaa ataataagtt
     6901 tgaagataat ctatataagg caacagacaa taaacaagtt aaaggtagaa aagaaatgag
     6961 taatcaaaat attaactcaa ataaagagga aaatagtgaa aatgagtgtc atttagatga
     7021 agataaaagt attcacttag aagatgatag tacatatgaa aaagacttta gtaagaataa
     7081 tgagatttta caacaattac tgaatctgtt aaaaaataat gacaaaaaag ataaagatat
     7141 aaactctata ttaaatgatt tagaaaaact aaatatatca gatgaattaa aacaagaact
     7201 taaacttaat aaaaatgtat tgaataagat tgtaaaccaa aatgctaaac taaacaatgc
     7261 taatataaat aactctgatg acatgcaaag tttagacatg atgaagttaa gagaactttt
     7321 agataatgat gctaataaga actttgaagt aattagtaaa actacagaaa atgatacaaa
     7381 aagcatagac ttgtacaatt ttgatagtaa tagaatggat aatattaact caaataagca
     7441 aaaagatgat agttctgata ttttggaaaa actagcaggg ggcaatggaa ataagagcgg
     7501 aaactttggt caagtgataa ataaaaatct tgatacgaat aaagttaaaa atgataatgt
     7561 caatttaaaa catatagaat caaatgtaat ggatgattct ataaaagcta ttaagcatat
     7621 gaaaacaaat gatatacaag aattaacaat taaattaaga ccaaaagaat taggtgatat
     7681 gaacatacaa ctgctaaaag atggtgaaaa tatgagagct gttgtaactg tttttaataa
     7741 agatgtattt gattctataa ataaaaatat gacagattta aagcaacatc ttgaacttac
     7801 aaatgtaaac ataaaagatg tatcagttca gatgcatagt gataatagaa atacatctga
     7861 tacttttgat aaagcatttg aacaacagaa tagacaaaat aaccaagaaa acatgaacaa
     7921 gcagaataca aataataagc gtaacaatat ggccatagaa gatgagatta aagaaaatat
     7981 tattgatgat gatagagtag atttattagc ttagtaaggg ggaagtattg tatgtacgga
     8041 gtagcaacag ctttggcagg tgctcaagga agctataggt caaataactt aagccaactt
     8101 gaaagaaata ctaatgacat tgcgaataaa caagttagaa gcaaaggaaa tcagacattg
     8161 acagataatg gaactccaat tataggacag ggtgatgatg ctgataaaga tttatttatg
     8221 aaaatacttg tagcacaaat gggtaatcaa gacccaatga atccacaaga cccaacagag
     8281 tatgtatcac aattagcaca gtttgcaact ttagaaaaga tgtctgctat gaatgaaaag
     8341 ctagatatac ttttagcttt aagcaactct actctaataa attctgcttt aagcatggca
     8401 acatcattaa taggcaaaaa agtagaggta tctggagaag agaaagaaga tgaaaacatt
     8461 gtaggtgcag taaagtccac atacataaaa gatggagagg tacacttaga gctgaaagta
     8521 gaaggcagtg ataaaactgt agatgttaaa tatgaaaaat tacttaaaat aggagaagta
     8581 gatacaaatt cagatgaaga agaaaattct ccagaatcag gtgaagaaga aagttctaca
     8641 gaacaagtat aatattaaac tagaaaagag gtatattcat gataaaagca atgtattcag
     8701 gtgtaagtgg gatgaaagca aatcaaacaa agctagatgt aataggtaac aatgtagcaa
     8761 atgcagggac tacttctttt aagaaaagtt ctgctagaat aacagattca ttttatcaaa
     8821 ctgtattata tgcaagtgct ccaacagctg cattaggagg aacaaactta ggtcaagtag
     8881 gtgtaggtag taagatatca agtataaata aagatatgac gcaaggaaat gtacagccaa
     8941 ctaatagacc atcggattta atgatagatg gagatggata tcttccagta gtaaggcaag
     9001 gtacagttat gtatacaaga gatggctctt ttaacttaga tacaggattt aaagcagcag
     9061 ctggaccttt aaaagatgtc aaaggcggaa gacttgtaaa tggtgatggg tatatgttac
     9121 aaggtatgat ttatgaggga gaatataaag cagatacagg agaatttgtt aaaaaaggtg
     9181 atgccaaagg aaaagaacct attgtaatac ctcttcaaag tataagtcct aaagatggaa
     9241 cagttcaaaa tgtattagag tataacatat caaaggatgg gacaatagaa tttttattga
     9301 gtgatggtca aagaactcag tttgtgtata ataatattcc tgatgatgct aaggtaggag
     9361 atacacctgc tggaatcgat aaattaaagg gaactattca aaaagtacaa acatatgcat
     9421 tccaaaatcc agcaggacta gatgcagagg gaggaaactt attcaaacca tcagcaaact
     9481 caggagaccc acaaatagca ggagctacag gaacaataat acaaggggca atagaagctt
     9541 ccaatgtaga tatagcagaa gaatttacag aaatgataat agcaagtagg tctttccaag
     9601 caaacagtaa aataatatcg acatcagacg agatacttca agaaatcata aatcttaaga
     9661 gatagtaggt acatattatg ataagagtaa cggatttaaa cggaagaaaa tacatattaa
     9721 attcagacct tatagtgagg attgattctg taccagaaag taggattctc cttacaacag
     9781 gagaaaaaaa ccttgtaaaa gagtcggtag atgagatagt agataaagta ataaagtata
     9841 aaagaaaaat aaatgtaggt tatattagga gcgaaaaata atgaaaaaaa cagattcact
     9901 gacatttatt ggactaatag tatctacagt attagtttta gttggtgccg caaaaggaag
     9961 tagttcaggg ttaaagaatt tttttgatgt ttcatcaata ttaatcacag tgcttggttc
    10021 atttggagct ctaatgatta catttactat agatgatata aaacttataa aaaatgcatt
    10081 acaatactca tttaagacaa tgagtgtatc aaagttagat ttactagaac aatttaaaac
    10141 actttctaaa aaagctagaa aagaaggtct tttatcaata gaaagtgatg taatgggaat
    10201 agaagatcca tttatgaaga aagggttaga gctatgtatt gatggattgg aaatcaatga
    10261 gataaggtcc atcttagaac tagagataga ttctgttgaa gatacaaata acagatactc
    10321 taaactattt aaaacttggg gaacctttgc acctgccttt gggatgttgg gaacactaat
    10381 aggtttaata caaatgttag ctgaccttac atctccagat ttaatagcat ctggtatggg
    10441 aaaggcatta attacaacgt tttatggttc tttacttgcc aatatcgctt tgaacccaat
    10501 tgcatataat atagatgaga agactgaaaa agaaatttat gtaaaggaaa tgatgttgga
    10561 aggtataata agcatacaat ctggggagag cagtatagta gttgaggaac gtttagccac
    10621 atacctttca aacaatgaga agttagaaat tatgaaatcc aataaaaata cagaaagagc
    10681 aatgagcaat ggcgcgtaaa aaaaataaga aaaaggatga gataaatcca gatgcatggc
    10741 tggctactta tgcagatact ataacactga ttcttacatt tttcgtatta ttgtatgcaa
    10801 catcagttgt agatatggaa aaatttaaaa acatagcaga tgcgttaaaa ggtcagtttt
    10861 ctgggacaag tatatttagt acgggttcaa gtacaagtga gaaatctcca atagacagtg
    10921 ttttagtgga taaaaatgat aaatcaaatt tagaacagga tttaaaagat aaagttaata
    10981 attctaactt acaaaatagt gtaacagtca aagatgatag tagagggatt ttactagagc
    11041 tagatgattc aattttattt gactctggga tttctgagtt aaaagtagat agtaaagcta
    11101 ctcttaataa agtatatgac atgataaaga tgatgaaaaa taagatagtt atagaaggcc
    11161 atactgataa tgtacctgta aaatcaagta cacatgaatc caattgggat ttatcttcta
    11221 gtagagcagt aagtgttgta agatactttg tagaagaaaa gggcatggac cctaagctat
    11281 tttcggcaac aggatgtgga gagtatcaac ctttggtttc aaatgaaaca gcaaagggca
    11341 gggctgagaa tagaagagtt aatatactta taactattga ctaggaggaa gaggtttgtt
    11401 caaaaataaa aaaattatta taattatttt gatactgatt attttctcag cgggagcagg
    11461 tttttttata ttaggagaag gtggaaataa acaaattgac aagattgcag gtttgtttat
    11521 tccaacagga aaaggtaaag atgaagtaaa gaccgaaaat cttgacttag aagagatggt
    11581 attaaagcta gctgatgaag gtgtaagata tttaaaagta tcaataactg tatcttatga
    11641 ttcttcatac aaagatatag aaaaaaatac gcctttaatt agggataata ttataaattg
    11701 ttttatggca aaaagagcag atgattttac tgctgagaat ttaaataaca taaaagaaga
    11761 aattaaaaat acattaaata tacaactagg tgaaaatgta attcaaaata tatatttcac
    11821 aaatatagta gtgcaataaa aggatggtaa gacatgatac tttatttact aaaaatatta
    11881 atatgtatcc caataatttt aatattgata gtcttatctt taaaaattag ccaaggaact
    11941 ttattaaaaa aatataatga taaatatgta aaaatactag atgttgtaaa tataagtaaa
    12001 aacaatagca taataatttt aaaaataggt gaagaaggat gcattatggc atcttcttcc
    12061 tctagcataa ataaaataaa agatttgtcc aaagaagaaa tattaaaaat agaaaatgac
    12121 attaaagaaa gaaattttaa aattcaagat tttaaaatgg aaagaattaa aaagaaatat
    12181 gatttcagta gatatattgg aaaatttaca aaaaaggaag ataagtaaat ggaactatta
    12241 tcaaaattac ctttggctga tgtaccatat acaagttcaa tgcaattgtt tttatttcta
    12301 actgcattaa tgtttttacc atttgtaatg ttcatgatga caagctttgt cagaatagtt
    12361 ataagtttat catttttgaa atcagcatta ggagcacagc aagcaattcc aagccaaatt
    12421 ttggtagggc ttgcaatagt attgacaata tttataatga gaccagtatt aaatgagata
    12481 aacgaaaagg cgttacaacc atatatgaaa gaagaagtta ctatggaaga agctatgaag
    12541 gaagcagaag gacctattaa agagttttta ttaaagcaaa ccagacagac agatttagat
    12601 ttatttgtag aacaggctgg tttaaaagaa aagaaattaa ctcgtgaaaa tataccacta
    12661 tctgttgtag ttccagcatt tgctataagc gaactaaaaa ctgcttttca aataggtttt
    12721 ttaatatata taccattttt gataatagac cttgttgtgg caagtgtttt gatgtctatg
    12781 ggtatgttta tgttgcctcc agtcatgata tcattaccgt ttaagttatt attatttgta
    12841 atggtggatg gatggaattt gatagtaaaa acattgatat tgggatttgg ataggagaaa
    12901 aatagtatga gtgaatttat gataatgagt gttcttaaaa atggcatgtt tacaggagct
    12961 atgattgcag ctccagtact cattgtttct cttgtagttg ggcttttaat aagtgtgttt
    13021 caagctacaa ctcaaatcca ggagcaaaca ttgacctttg taccaaaact aatagctatt
    13081 cttttagtag tagctattgg tggaagctgg atgttacata ctcttattag ctttacctct
    13141 gagatattca atatggtatc tcttataact aaataagtag ggtatatatg atttttgtat
    13201 ttattagaat agtagcattc tttggaacac taagaatact atttccagct ggtacacctg
    13261 cgttatttaa gtcactcttt gcaatcatta tatcaatatt gatttcaagt actatgcaaa
    13321 ttgaatatgc aacaaatata gataacattg tattgtttac cttatatggt ataaatgaga
    13381 ccataactgg gattatgctt ggatatataa ctaacctatg tttttatagt atcagaatgg
    13441 cgggttctat gatggaccaa cagatgggat tatcaatgat taatatgttt gatccaaact
    13501 cactgactca aacaacttta attgataatc ttatgaactg gacagcactt atgatatttt
    13561 ttagcatgga tggtcatcat gttttaattc gaggtataag gtatagtttt gagttaattc
    13621 caatagggaa atcattcata gataataata tagattatat aataaacata tttgtgcagt
    13681 gctttttaac tggattcaaa atagcaatac ctattgtact ttgtctgctt atggcagatt
    13741 ttattttagg gcttatatca agaagtgtac ctcaattaaa tgtaatgata gttggtatgc
    13801 cattaaaaat attggttggt attgcattat ttatcatatc aataccttta attgtcaatc
    13861 aaataagtca tttattaagt caaataccaa agatgtatga gggaactttt gcactagctc
    13921 ctatgttttt tatgggttca gcagataaaa cagaagaagc tactccaaag aaaaagggag
    13981 aacaaagaaa gaaaggtaat atagcaaaga gtagagaatt acccgttgca atgactctac
    14041 ttgcattcac cttattagta cctacattat tttcatatgt agtagataca cttaaatctt
    14101 cgcttaatta ttttttgagt ctagattttt atatgaacat aaattattca aacctagaga
    14161 aattagttat agcaggatta atagattttt ttaagatatt tttaccaata gctattccct
    14221 ttttagtttt aggcataata gcaaacctat ttcaggtggg aatactgttt actggtgaaa
    14281 ctttaaaacc aaatttatca aaattaaatc cagttaatgg attcaaaaac atgttttcta
    14341 tgagaagttt gtcaacctta ataaaggata cagcgattat atctattttg gcttacatag
    14401 gatatacatt cttccaagat aattatttag atatattaaa gctaggtaat atatacctac
    14461 caactttaat gtacacagtt aaagacttag tatattcaat attgagtaag atttgtgtag
    14521 ctatgatagc tatagcagtt gcagattatg tctatcaaag atatagtcat aaaaagcaac
    14581 tgagaatgac aaagcaagaa gttaaagatg aatataaaaa ctcagaagga gacccagaag
    14641 taaaagctaa gataaaacaa aaacaaagac aaatttcgtc tcaaaggaca atgcaagcag
    14701 taccaagtgc tactgtaata gtaaccaatc caacccactt atctatagca gtgagatatg
    14761 aaaaagggaa agaccaagct ccagtagtgg tggcaaaagg tgcagattat ttagctttta
    14821 aaataaggga aattgcaaag ggaaatgaca tccctataat agaaaacaaa cctattgcta
    14881 ggttgttata taagcaagta gaaatagacc aagaaattcc agaggacatg tatcaagctt
    14941 ttgcagagat attagtagca gtatataaaa ttaaaaatag atacaaagtc cccaaaaggt
    15001 aggataagta atgaataagt ttaatttaaa ggaaaatatg gatgtaatag tggcatttgg
    15061 tgttgtaggg attgttctta tgatgatagt gccattacct acatttatat tagatatttt
    15121 actggcaatt aacataagtt tatctgtgct aattttacta atgacattat ttacaacaaa
    15181 tgtacttgat ttatctatat ttccaacagt gttacttgtg acaacactat ttagactggg
    15241 attaaatctt tcatcaacta gattgatttt aacacaggga tatgcaggtg aagttataga
    15301 ggcatttgga agttttgttg taggtggaaa ttacgtagta ggaataatta tattcttaat
    15361 tatattaatt gttcaatttg ttgttataac taatggttct ggaagagtat ctgaagtaac
    15421 agctagattt acgttagatg ccatgccagg taagcagatg agtatagatg cagatttaaa
    15481 tgcaggagtt atagatgaca aaactgctag aaaaagaaga aaagagcttc aacaagaagc
    15541 agatttttat ggttctatgg atggagcaaa taagtttgta aagggagatg ccatagcagg
    15601 tcttatagta acagcaataa acataatagg tggaattgta ataggagctg taatgctgaa
    15661 tatgactctt atgggtgcag cgcagacata tgtaagactt actatagggg atggtcttgt
    15721 aagccagata cctgctctat taatatctac atcagcaggt ataatcgtaa caagagcttc
    15781 tactgatgaa aactttggtg gattagtagg taaacagtta acggcaatac caaaagcagt
    15841 aatgatgact ggttgtgttc taatcgtact tggtatgatg cctggattac ctaaaccagc
    15901 atttttttca ctaggtatag gagctatagc agcaggttac tttttaggaa gagaaaagaa
    15961 agatacttta gaagacttgg agtttgaaaa acctgatatg gtaaatccag ataaaaatga
    16021 agtagaagat gtaacaaact taataaatgt agaatcaata gaagtagaaa ttggatatgg
    16081 tttaatatct ttagctgatg aatcagcagg aggagattta cttcagagaa tagcatcaat
    16141 aagaagacaa tgtgcaatag acatgggtat agtcgtacaa cccataagaa taagagataa
    16201 cttacagtta aatccaaacc aatacaatat aaaaataaaa ggaaatgtag ttggtgggta
    16261 tgagataatg cctaatatgg ttttatgtat ggaccctatg aatcaaggat ttagtatatt
    16321 tggaataaag acaattgaac ctacttttgg aatagaggca ctttggatag aaagtgacaa
    16381 ggtagaagat gcagagttaa agggttatac agttgtagat tcatccacga tacttataac
    16441 tcacttacta gatataataa aatcaaatgc acacgaatta ctgggaagac aagaagttaa
    16501 gactattata gatgcagcaa gagaaaatta tagtgctgta gtagatgaat tgataccaga
    16561 tttaatgaca cttggtgaaa ttcaaaaagt acttaaaaat ctgttaagag aaaaagtaaa
    16621 tataaaggat agggttacaa tacttgaaac gcttgcagac cagtctagaa atacaaaaga
    16681 tatagagctt cttactgaat atgtaagaat agccttggct agaagtattt gtaataattt
    16741 agttgatgat gataagacaa ttgtagtggc tacattgtct ccagaaactg aggagttggt
    16801 atctaataat ttacaaaggt ctgtaaatgg aacttatcca gcagttgacc cagaaaatac
    16861 aaataaaata tttgaaagta tacaagaagt aatgagcaat gtatatttca atcataatat
    16921 accagtaata gtagtatcac caaagataag agctccattt agaaaattag ttgaaattgt
    16981 atatcctaat ttgactgtat tatcattaaa tgaaatacct aacgatatta aaatcaaagc
    17041 agaaggtgtg gtgagcatat atgatgacga aaaagtatac agctaacaca attcaggatg
    17101 ctatgaatct agccaaaatg gaattgggtg acaatattac attaatagat aaaaaagagg
    17161 taagaaagtc tggaatacaa ggtatctttt taaagaaaga tattgaacta actattggat
    17221 gggaaaagag agaaaacgta gaacaaaaag atttaaaacg agagatagaa caattaaagt
    17281 caattattaa caatatgggg tttgataata agaatgatat tgatatagat aaaatatgta
    17341 aaaatctact aagtttagaa ttaaatgaag aaattgtaga atctataaga attgatttgc
    17401 aagaaatgaa gttcaatgga attgatacaa gcaaaaattt agtagaaatt ttaaagaaaa
    17461 agattaaaat agaaaatcaa gcaataaatg ggaaaattgc tctagtcgga ccaccaggag
    17521 taggtaaaac tactacaata gcaaagttag ccgcaaaatt agtttttgaa gaaaataaaa
    17581 aagtaggagt aataactata gatacataca ggattggtgc tgtagagcag ttgaagatat
    17641 atactgatat aatgaatatt ccttttaaag gggtaataag tcctgatgaa atggaattgg
    17701 cacttgatga gatgaaagat tgtgatgttg tattaataga tacaacaggt agaggctata
    17761 aaaactctat gcaaatacta gaaatcaaaa atcttataga taaagctgaa actgataata
    17821 tacatttagt tttgaattgt acaacaagag aaagtgatac aaaagcaatt atagattcat
    17881 atagaaatgt gaattttaaa agcttaataa ttacaaagct ggatgaaaca ataacatatg
    17941 gctcaatatt taacatagtg aattatgcac aaaaaccaat atcatatatt acaacaggac
    18001 aaaatgttcc tgatgatatt ataaaaccta atgaagataa gattataagg ttactattag
    18061 gagttgaaag tatatgatag accaagctca aattttgaga aaaatggcaa tggaaaaaag
    18121 aggttctgat gagattatag tagagaatga gaatacgcct aagataatta caatagcttc
    18181 tggtaaaggc ggtgttggaa agagtaatct cgctacaaat ttgtctatat gcttgaccaa
    18241 acttaataaa aaagttctta tattagatgc agacatagga atgtctaata tagatataat
    18301 aatgggagta aatgtaaaag ggactataat tgatgtaatt aatggtgaaa aaaatataga
    18361 agatataatt tcacagacta agtatggtgt aaatataata tctggaggtt ctgcccttaa
    18421 tcatatagaa gactttacag aagctcaaag aaataagttt atacatagca tagagcagat
    18481 tcacgatgtg gattttataa taattgatac aggagctggt atgagcaaaa gcctattgtc
    18541 atttatatac tgtagtactg aatttttctt aataacaact ccagaaccga catcattgac
    18601 tgacgcatat agtttattaa aagctataag taattttggc ataaagagaa gtgcaaatat
    18661 aataatcaat agggtaattg atttagaaga agcaaaatct acatacaaaa gaataaagat
    18721 ggttgtggat aaatttctaa accttaatct ggaattgtat ggatatatag tagatgacaa
    18781 aaaagtaggt ttatgtgtaa aaaaacagat accattcatt ttagaatatc ctacaagtat
    18841 tgcatcaaaa tgtattatag ccatagcaaa gagaatggta agtcagtcaa gagaagatat
    18901 tgttgataat aaaagttttc taagaaagat gtttagtata tttaaaagct aaggaggcgt
    18961 agttaatgaa tagagaagaa ttaataaaag agaatatgcc tcttgtaaag agtatagcaa
    19021 gaaaattttt tataccaggg aaaggttttg aatatcaaga cttagtaaat agtggagtca
    19081 ttggattgat tgatgcaata aataaatttg atgttgaaaa aggggcaaag ttttctagtt
    19141 actcttatat aaagataaaa tctgctatat tagatgaaat tagaaatcaa agtccgattt
    19201 ctaaacataa tctaactaaa gtaaataagt ataatagagt ggtagaaaag ttgcaatcta
    19261 aattattaag agagccaact tcatatgaaa ttgcaaaaga attaaaggtt tcagaaaaag
    19321 aacttcatga tatcgaaagt aatatagaca tgttaaatat tgtatcatta aactatgtaa
    19381 tctttgaaga tacaaatgaa actgtacaag atgtaataag tgatagagaa gaggaagctc
    19441 cagaaaatat aatagaagaa gaagaaaagt tagagattct atctaaagca attagtaatt
    19501 taaatgaaag agaaaaatta atactttcac tgtactatta tgaagattta aatctaaagg
    19561 agatagggaa agtgctaggt gtttcagaat caagagtttc acaactccac agaaaatcaa
    19621 taagaaactt aagaaacaaa ataaaagaac ttaaatattc tatatagatg gtgattgata
    19681 agatgataca aattatatta atagtagtgt ctattttaat aatagtaact atagttataa
    19741 atttacataa ggaatcaaaa aataaagata acagatactt tgataaaata tttgagcaag
    19801 aatatgaagc aaatgataga ttaataattg cagaaaaaag aagattcttt ttagacaaag
    19861 tacttgctga aataagaagt aataatcata taaaagttta tgaaaattat aacctaataa
    19921 aggctaatga agaaagaaag gaaagcatat ttatagaatc aaaaaaggaa gtaagacaag
    19981 aaaatgagtt atctcttaaa gtgaaacatc ttataagttc aggttatgat gatgtggaaa
    20041 tatgtaagat gctagatatt ggaaaggggg aactgtcatt aataaggagt tgttacaaaa
    20101 tataaggata ttattatgtg ataaaaaagt attcttagga ataggtattg gtatattaat
    20161 atcaacttta tgtataatgc tttttagtaa taatgatatg agcaaggcag agatagaaaa
    20221 aaaggccaga gcgttaggta tgaaatatac aagtgagatg aaagttttag aatagatgac
    20281 actgtttaaa catttacaaa aaaggaggtc ataatgtgat aagaggatta tatacagcag
    20341 tttctgctat gataactaat caacaaaagc aaaatgttgt tgtcaataac ctagctaata
    20401 tggatacaaa tggatataaa agtaaacaac ttctgactaa aagttttgat gaattgaagt
    20461 tggaaggtta caataactac gcaaatggaa ctaaacaaaa gcaggttata ggcgatataa
    20521 gtccaggtat ttctatggat gaaactatca caaactttaa tcaaggacct ataaaaacta
    20581 ctgataataa aatggatgta gctattcaag gtaagggatt ttttctagtt agtgatgcag
    20641 ctggaaatca attttacact agaaatggga acttcagaac agacaatcaa ggtgacctta
    20701 taacaagtga agggtattat gtacttggga cgaatactgc tactggtgct gtaggtcata
    20761 taaatgtagg aaatcaaaaa tttgaagtgg caaaagataa tacaatatca ctagacggaa
    20821 atgctatgta taaatttaat attgtagatt ttaataatta caataactta aaaaaagaag
    20881 gagataactt gtattctgga ggaggagcta tgaaagtaaa taatgcactt acaatacaaa
    20941 gtgcgataga gtcttctaat gtagatatgg tatctgaaat taataatatg atgacaatat
    21001 caagagaata tgaggcaaat caaaagataa ttcaggctat ggattctaaa ttagcaaaaa
    21061 tagcaagtga gattggttca gtaagatagg taataggagg tgaattttta tgtacacaat
    21121 gttttatact agtaaaacag ctttatcagc aaatcaaagt aaactagata taatatctaa
    21181 caatatagca aattctgatt cgacaggata taaaaaaata aatatggaat attctgattt
    21241 agtggatgaa gcactaaata gaccatcata tcctacaaat ggcaatgata tatcgactgg
    21301 aacaggagct aaggcaacag ctcccatgag aatttatgaa caaggtgcat tggctcctac
    21361 agatagtaag agtaatttgg cgatagatgg agaaggattc tttagagtga taagaaaaga
    21421 tggtacatat gcatatacaa gaaatggtgg atttaatgta gatgctttag gtaagattgt
    21481 agatgataat ggaaatatat tagatgttca atttgaggca ggatataact ataataatac
    21541 gaatatagat agtacaaacc tgacagtttc aagaaatgga gagctattcg tagataataa
    21601 aaaaatagga aaaatcaact tatatcaacc tataggaact caaaatttta tatctgaagg
    21661 agatagttta ttcgtcgcaa ctgatgcagc acagattaaa caagttgaga aagtagatat
    21721 agttcaaggc tatagagaga ggtcaaatgt ttctttacaa gaagaatttg ttgacttaat
    21781 agccactcaa agagcatttc aaatgaactc aaagggaata aagacagcag atgaaatgtg
    21841 gcaaatagct aataatttaa gagctaaata attatgtaag gtgaggaata ttcatgaatg
    21901 atatgaaaga gtacgatttt ggcaaacctc agagttattc aagagaaaat ttaacttgtt
    21961 taaatttttt attagctgaa ttttgtaaaa aatataaaaa ctatataatt tatgaactta
    22021 agtgtaattc tagtatggtt gttgatagta tagaccaagt aaattatcag acttttttag
    22081 atagagtaac ttcttcatgt gtagtagtac aaaatgcaat ggaacctgtt atgaaaaact
    22141 tttcatttag aatagataga agtgtagctg atatgtggat tgatataact tctggaggaa
    22201 ctggagttgt aagagataat gatagagagc ttacagaact tgataaaaaa gtgcttttac
    22261 atcttatgga tgatttagta agaaatatgc acttatttga gggttttgaa aatatacaag
    22321 tattggatac acatacacat attaatttgc cacaattatg ctctccaact gcaccagtat
    22381 gcgttgtaga tttaaaagtg ctaaatgata atgagtatat tggaagtgtt tctctttgtt
    22441 ttccatacaa tggtttagag gctctattag aaagtatatc tgttatagaa ttttttgata
    22501 atgatgtagg agatgattca gaggaattta ccaaaaaaat ctataatagt gtatctaata
    22561 tagatttatc agttattgca gagataggaa aagtatcaat taatgttgag gatttactta
    22621 aactagaagt tggagatgta atagtaacaa ataaaaaaat aaatgattat atagatatat
    22681 ttgtagaaaa ttcaaaatca tacacagcaa caccaggatt tatatcaagc aaaaaaggtg
    22741 taaaaataaa tgatgctgtt ggaaaagagg tgtagttatg gacaatagta atcttatttc
    22801 agatgaagaa gcaaagatgt tattaaatga taaagttgaa agtgaagttg atggagatga
    22861 tgagttagcc ttagatgtct ctgaagttaa tcgaaataat atacaaagga tacttgacct
    22921 taagttagaa ttatctgtgg ttataggaag aactaagatg actctaagag atattttaaa
    22981 tttacataaa ggttctctta ttgaactgga tactttagca gaccaagatg tggaaatact
    23041 aatagataat aaagtattgg cttatggaaa agtagtggtg gtagatttaa attttggtgt
    23101 aaagataaca agtatagtaa gtgaagaaga catggtaaaa tctcttgttt agagataaca
    23161 ttaattaata cttaaggcat gaggtggtac ttattttaaa cttaaatgat ataaaaaaag
    23221 atatattaaa attcaataaa aaagactcat gttctctata taatgaatct attaataata
    23281 taaataataa ttgtatatca actggattga aatttttaaa acaagcaaga gatttaaacc
    23341 cagatgatgt tgatatatta aatctcacag gtcttgtaaa cttactaaaa tgtaattttg
    23401 atgatgctgt agaaagtttc tataagagtt cactttgtga aaaaactgct ctgtgtgaaa
    23461 agtatataaa catgcttaca tctgaggagt ttttaatttt ctttgaaaag tacaatcaac
    23521 ttataaggtt aataaacaaa ggaatgtata aagaagctat acaagggttt aaacttataa
    23581 ctgaccaata ctgtgattta atagaacctt atgagttgct tgccttactg tatgacaagg
    23641 aagaagaata tataaaattt gatgaatgtt tagaagtatt gaagattatt gataaggaaa
    23701 attatttatt aaatcaagaa aataacatat tagatatgta taatagtgct aatatacaaa
    23761 ctaaaagtaa tataaaagta gatgattata agaatgagtt tttacataaa agtagtaata
    23821 atcataagat taatgattat aataatgtaa ctaaagaaaa gagttcaagc aaaaaccaga
    23881 aaagttcaga ttctaataag aaaaaaagta ttaaaaaaaa taagtaccta tatatagtca
    23941 taatattagg aatattatta gtaggacaaa gtatatatag ttcttataag atgaataatt
    24001 taactgataa aatatctaaa aataatgaaa ctaaaaatga agggtcaata gattctagtt
    24061 tggataagaa agaaaataat ataaaagaaa atgaagatat gaataagact tctaatatca
    24121 ataagagtaa taaaaatatt aataaagagg atgtaaaatc taaaaaaaat actgtagaaa
    24181 atgaagatga taaagaatct gatttattta cagaggatga attgatgtct aaggcaaata
    24241 ctttaaaatc agaaaagaaa actaaatctt ctatacaact atataagaaa gttgcagata
    24301 taggtgaaaa taaagggaat acatctgaag caacttatca agttgctatt ctcagtgaaa
    24361 agttaaaaga ctataaaact gctgaagaat attataaaat gtatgtagaa aattactcag
    24421 aaaaagatgc ttattttgat gaatcttatt ataatttagg tatgatgtac tataacaatg
    24481 gtgatttaaa aagttctaaa ttgacattaa agaagttggt taataaggta ccaaatagta
    24541 tgtataataa ttcaaaagta aaagaaattt
//
LOCUS       DAEPXF010000044        24484 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain HB27
            SAMN13376389-rid12479423.denovo.046, whole genome shotgun sequence.
ACCESSION   DAEPXF010000044 DAEPXF010000000
VERSION     DAEPXF010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13376389
            Sequence Read Archive: SRR10518432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 24484)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24484)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001069624.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001069624.1
            Genome Coverage       :: 315x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/15/2021 01:54:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,165
            CDSs (total)                      :: 4,076
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 89
            rRNAs                             :: 10, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 10 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 19 of 69
            Pseudo Genes (incomplete)         :: 44 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 10 of 69
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24484
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13376389-rid12479423.denovo.046"
                     /strain="HB27"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="China: Hebei"
                     /collection_date="2017"
     gene            78..194
                     /gene="rrf"
                     /locus_tag="KRL67_001547"
     rRNA            78..194
                     /gene="rrf"
                     /locus_tag="KRL67_001547"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            313..504
                     /locus_tag="KRL67_001548"
     CDS             313..504
                     /locus_tag="KRL67_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF378 domain-containing protein"
                     /protein_id="HBH1522435.1"
                     /translation="MKKVALILAIIGALNWGAIGILGTDLIGSIFGGTYEMVSRIIYF
                     IVGLAGLYLIPSLMSDDRE"
     gene            659..2068
                     /locus_tag="KRL67_001549"
     CDS             659..2068
                     /locus_tag="KRL67_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HBH1522436.1"
                     /translation="MFIINELNKIVNEEVISFHMPGHKKGQIYKKLGYKDILENLYKM
                     DTTEILGTDNLHFPEGIIKNSQEKASRVFKSDYTCYLVNGSSCGIQSAIMSVCRPKSK
                     IITNRDCHQSVINGCILGDIDIEYVPCEVSEETNVLKGISISNVIDIVDKNLDAKAVL
                     LTYPTYYGMTYDLEYICNYAHSKKMVVIVDEAHGAHLGLSERLPKTALEQGADIVVQS
                     THKTLPSFTQSSMIHIQGDRVDIDKILNMLRITESSSPSYLLLSSLELAVDIYENKGK
                     ELMEELLNNIRTFKNNVDSNINIYDTNDKTKIFISSKNMGLTGYELESILRNKYNIQV
                     ELSNYYGVLLICTIGNTAKDFSSLETALNNVIKEDFKFEKLENKKYPVVIPKKILTPR
                     EAFYKTKKSVKIYDSIGKICGECIVPYPPGISVISPGEVISKEIIDYLMFCHSKGMIV
                     SGLKDVDLNFIQVIDLEDI"
     gene            2189..2878
                     /locus_tag="KRL67_001550"
     CDS             2189..2878
                     /locus_tag="KRL67_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate kinase"
                     /protein_id="HBH1522437.1"
                     /translation="MKGKLIIIESGSDASGKATQTEILYERLKKEGYMIKKVEYPNYE
                     SPSSALVKMYLKGDFGKNASDVDPYIASTFFTADRYASFKTDWEEFYNEGGIIIADRY
                     TTSNMVHQASKMDICDREEYLNWLADYEFNLFKIPKPDCVIFLDVPIEFSEKLMENRK
                     NKITGEEKKDIHESDIEYLEKSYNNALYIADKYDWKKINCVLDNKLRSIESIHNEIYK
                     IVLDSIKSMER"
     gene            2881..3816
                     /locus_tag="KRL67_001551"
     CDS             2881..3816
                     /locus_tag="KRL67_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HBH1522438.1"
                     /translation="MYFENIIGQDFAKKYLTNSIKKNKLNNAYMFEGMDGIGKKKFAD
                     ELSKLLLDYENLENSPDYVLIKPDGNSIKIAQIRNLQSDIVIRPHKDYKIYIINNAEK
                     MTVEAQNALLKTLEEPPNYAIIILVTNNKESLLETIKSRCDIIKFSPIPIEDLKRYLI
                     NTGIEEERAQLLAIFSRGSIENALNLSQSSEFSMMREDIQQYIQIMLDKNIVEILNIP
                     NNMEKYRGKIIALLDMMINYFRDIIILKENVNKNMLINVDKLVFIQNMSGKISYSQLS
                     KIIDIIEDAKSKIKSNCNFNISIQVMSLNIYEVIK"
     gene            3813..4706
                     /locus_tag="KRL67_001552"
     CDS             3813..4706
                     /locus_tag="KRL67_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage 0 sporulation family protein"
                     /protein_id="HBH1522439.1"
                     /translation="MIKIVGIRFKSAGKIYYFDPVDFNIEQDMDVVVETARGLEYGKV
                     VVGPKDMDESELVSPLKPIIRIATEEDKKVYLENKEKAKETFELCQQKIKEHELTMFL
                     IDCEYTFDRNKLIFYFTAEGRIDFRELVKDLAAIFKTRIELRQIGVRDEAKSIGGLGP
                     CGRKLCCSSWLGDFQPVSIKMAKDQSLSLNPTKISGICGRLFCCLKYEHDVYSEAIEK
                     MPVVGALVQSDDGKGKVVEVNPLLEQIKVEFQDKTVKIYTREDIKILREPKKCDGCGG
                     KCKDNDGLDEATLRELKKLED"
     gene            4784..5530
                     /locus_tag="KRL67_001553"
     CDS             4784..5530
                     /locus_tag="KRL67_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA1(Val) (adenine(37)-N6)-methyltransferase"
                     /protein_id="HBH1522440.1"
                     /translation="MQLKDSERIDDLQLKGLKIIQDTNGFCFGIDAVLLANFTKIKKD
                     AKVVDLGTGTGIIPILIAGKSEAKKIIGVEIQEDVYEMATRSVKLNGLEERVKIINED
                     IKYIDKVLDVNGYHVVTSNPPYMHIDGIKNPNDKKAISRHEVRCNLEDVIRAASRLVM
                     PRGKFFMIHRPTRLVDIITLGRKYKLEPKVIQFVHPRPQKAPNLVLVQFVKDGRPELK
                     ILDPLYVYGEDGNYTKELKAIYNNKDIGEL"
     gene            5531..6364
                     /gene="rsmI"
                     /locus_tag="KRL67_001554"
     CDS             5531..6364
                     /gene="rsmI"
                     /locus_tag="KRL67_001554"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HBH1522441.1"
                     /translation="MSGKLYICPTPIGNLEDITYRTLRILKEVDLIAAEDTRHSIKLL
                     NHFEISKPLTSYHEHNKDSKGDYLINKLIDGENIALISDAGMPGISDPGEEIIKQAIQ
                     NNIEIEVLPGATAFVTALVGSGMDTHKFVFEGFLDRDKKVRKAQLEELKEESRTIIFY
                     ESPHRLKDTLKDMLKILGNRNISINRELTKKYQEIIRENIETTIKIFEEKEVRGEFVL
                     IVEGFHGEKSKKENYDGLTDREYVLKLIESGMNKKDAIKIVCKDRKLKKDVVYKQVLD
                     L"
     gene            6387..7091
                     /locus_tag="KRL67_001555"
     CDS             6387..7091
                     /locus_tag="KRL67_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HBH1522442.1"
                     /translation="MEIGKIIIESLKREDTHKIFIELDNKHELEKIIPKISCMKSVGE
                     CKYHVVNCFEHSINALKELEIVLNDKDFFPIHLRKHVSNYLNTYTEKGINKLHVLKLG
                     TFLHDIGKPDSMTLDETGRVHFTNHEKIGAQIIDNMGIKLELSTETYRLISKYVRYHM
                     ILLSLYKKNDLSRKELINVFNLVDEDTIGVIMLGYADIVSTVKLLGKSGEVSVLKTYM
                     EYILTNYLYKSNYTHV"
     gene            complement(7279..7707)
                     /locus_tag="KRL67_001556"
     CDS             complement(7279..7707)
                     /locus_tag="KRL67_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007287587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HBH1522443.1"
                     /translation="MDVTDYRIIEILQDDGRISMKDLGKIVGLTSPAVSERVKRLEES
                     GVIEGYKAIVNPDSLGRVIKAFIHISLPSNGYTEFIESAAKDPRIVECHHITGDDCLL
                     LKVIVKDMYELENVIDTIKKIGSTKTSVILSTPIQAKSIL"
     gene            complement(7744..9174)
                     /locus_tag="KRL67_001557"
     CDS             complement(7744..9174)
                     /locus_tag="KRL67_001557"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HBH1522444.1"
                     /translation="MRNLTLLTDLYQLTMLNGYFEKNIHEDIVVFDMFFRKNACDGGY
                     TIVCGIDQVVEYIDNLHFSDEDLEYLKNLNLFSDKFLKFLKEFKFTGDIYAVEEGTIM
                     FPNEPLITVKAPLYQAQLIETALLTIVNFQSLIATKASRVCFAAQGDPVFEFGLRRAQ
                     GPDAGIYGARAAVVGGCAGTANVLAGKMFDIPIIGTQAHSWVQKFDNELEAFQAYADV
                     YPDKCLLLVDTYDVLNSGVPNAIKVFKNISEKGYKPMGIRLDSGDLAYLSKEAKKQLD
                     NAGFSDISITASNDLDEYTITSLKAEGATINSWGVGTKLITSFDSPSLGGVYKLAASC
                     EKGVLEPKIKISENPEKINNPGYKKVIRIYNEDNKAEADLIMLHDEVIDESKPLEIFH
                     PTYTWKTKVFTNYKVKELLKPLYIKGRCKYNKKAVLEIKNHVQYELSTIWEQYKRLSK
                     PHIYKVDLSRNLWYLKTQMIDSKKVL"
     gene            9306..9890
                     /locus_tag="KRL67_001558"
     CDS             9306..9890
                     /locus_tag="KRL67_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore maturation protein A"
                     /protein_id="HBH1522445.1"
                     /translation="MIIIGKIWFYMVSIGIIGSLISKNMGELNKVILSETSKGIEFAI
                     SLAGIMALWMGIMNIAKESGLIEKIGQKLNPIMRRLFPSIPKGHKAMSYIVMNIALNM
                     LGAGNGATAFGLKAMSELQTLNNKKDTATNDMIMFMVINISSIQIIPFTIIKLRMDMG
                     AQNPSEIIFTTLFATIISTAVAIITCKFFQRRYR"
     gene            9906..10427
                     /locus_tag="KRL67_001559"
     CDS             9906..10427
                     /locus_tag="KRL67_001559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore maturation protein"
                     /protein_id="HBH1522446.1"
                     /translation="MEIFSNLLIPIIILYIVVYGKYKKIDVYDSFVKGAIDGLKAAWD
                     ILPYIIGIFLAIGIFKTGKGLDMLEWLFTPIANMLSIPKELIGLISVKPLSGSGALGM
                     YSELANRVGIGSLVEKMGATIVGSSETIFYTMAIYYGSLKIKNTRHTLSCAMISHVAG
                     VIAAVFICYVIFV"
     misc_binding    10493..10739
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            10823..12760
                     /gene="metG"
                     /locus_tag="KRL67_001560"
     CDS             10823..12760
                     /gene="metG"
                     /locus_tag="KRL67_001560"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HBH1522447.1"
                     /translation="MSKPSFYVTTPIYYPSGGLHIGHTYSTVAADTIARFKRFCGYDV
                     KFLTGTDEHGEKIQKKAIEQGMSEIEYLDGMIKDIKALWNTMDISYDDFIRTTEKRHT
                     DIIQKIFTKLYEQGDIYKGEYEGRYCTPCESFWTESQLLEGNKCPDCGRETYLVKEES
                     YFFRLSKYEDRLKELFKDDSFCFPAARKNEMVANFLDKGLEDLSVTRTTFDWGIKVPF
                     DEKHVIYVWVDALCNYITALGYMTDNDEEFRKYWPANVQIVGKEIVRFHTIIWPALLM
                     ALGLEVPKQVFGHGWILFADDKMSKSKGNVVYPEPIIERYGIDTLKYFLLREFAFGQD
                     GSYTHRNFVTRINYDLANDLGNLISRTVAMVEKYNNGIIPTAKVSTDFDADLKEQAVS
                     TRENFEAEMDKMQFHEALESVWKLVRRTNKYIDETMPWALAKDETKKDELDTVLYNLC
                     ESIRIIATLINPIMNETANKIYEHIGIKGQDDITTWESTKTFGLIGENVKVFKGEPLF
                     PRLDVEKEIEELTKMFSGKPPVEEKPLEHKEEITIDDLDKIELRVGKIISCEKHPKAN
                     KLLVSQVKIGPETRQIVSGIAEYYKPEDLVGKEVTVVCNLKPVKLRGVESQGMILAAG
                     DDGDPYVLPFTQGAKDGCEVR"
     gene            12790..13560
                     /locus_tag="KRL67_001561"
     CDS             12790..13560
                     /locus_tag="KRL67_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HBH1522448.1"
                     /translation="MLFDSHAHLNDESFDEDRDELIGSLKDKGVDLVVNPGADIETSI
                     TAIELAKKYDFIYSAVGVHPHDVSKLDDTAIETLRKLATENEKVVAIGEIGLDYYYDY
                     SPREEQKEWFKKQIELANELKLPIIIHDRDAHGDTFEIIKNTKNPEIGCVLHCYSGNV
                     ELAREYVKMGCYISIPGTVTFKNNKKTREVVREIPLERLFIETDSPYMSPEPHRGKRN
                     NPSQVSFVADKIAQEKGISYEEVCRATKENAKKFFNIK"
     gene            13904..14335
                     /gene="phnG"
                     /locus_tag="KRL67_001562"
     CDS             13904..14335
                     /gene="phnG"
                     /locus_tag="KRL67_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnG"
                     /protein_id="HBH1522449.1"
                     /translation="MYTVDSTKILINMNLEKLEKIYSMIKDTSSISIIKEPNLATVMV
                     RANESVKNTTFNLGEILVTECSVKVDESLGYGIVSENNNKKAIYLAVIDAVLHSNNSK
                     FDELKNYINKSVYEENLRYEQEIIDEFSLINKTKVQFNTMD"
     gene            14350..14925
                     /gene="phnH"
                     /locus_tag="KRL67_001563"
     CDS             14350..14925
                     /gene="phnH"
                     /locus_tag="KRL67_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnH"
                     /protein_id="HBH1522450.1"
                     /translation="MVNYTQEIYRKMLDSASFPGKLNNIDNINIENNTKLLNGAIGIA
                     ITLLDQEVTFYIENYTQEDIKNIRALTMSNQIDYRKSDYIFLDINSEVDILQIKIGSF
                     EYPDESATIVHQVNDLSTYSQTKYIKLDLSGPGIKSKNSIYINGVREEFIERLSTINK
                     DYPIGIDLILVDKKGELAFIPRSSKLSWEVL"
     gene            14928..16013
                     /locus_tag="KRL67_001564"
     CDS             14928..16013
                     /locus_tag="KRL67_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-phosphorus lyase complex subunit PhnI"
                     /protein_id="HBH1522451.1"
                     /translation="MAYVAVKGGEEAILQAKNILEFYRVKGDSLPIDVKQIEEQMSYA
                     VDKVMSEGSLYNKELSALAIKQAQGDILEASFYVRALRSTMPRITYSEPINTEEMRIK
                     RRISATFKDIPGGQYLGATRDYENRILNVDLLEEDEKKNRDFKDSFINELETVDEIPE
                     FTKISNIFRNENLLQEEVCENKSEEVIDDITRNSLRFPVSRSAKLQALTRGESGVVSG
                     FAYAIVRGFGDEHPYISELRTGNVFIKVKHPIYEDEEIIIGDMLVTECEIVSKASQNK
                     TENSKTNSEDKNKMSLGYGLCMGNNENKAISMAILDKSLEPNSEKLAQDEEFVLLHID
                     GIDSLGFISHFKLPHYVDFKADVERIK"
     gene            16033..16902
                     /locus_tag="KRL67_001565"
     CDS             16033..16902
                     /locus_tag="KRL67_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate 5-phosphate
                     C-P-lyase PhnJ"
                     /protein_id="HBH1522452.1"
                     /translation="MIYNYGFLDENTKKEIRRKILKAVSIPGYQVPFGSRELPIAKGW
                     GTGGLQLTLSLIGKNDVVKVIDQGSDDSTNACNIRNFIASVSNVETTKDTLEATLIQT
                     RHRIPEEKLKSNQILIFQVPIPETLRIVEPSEVETRRMHSEEDYSRMWVYLYEDIVRF
                     NDISIAVEYPCKVNDRYLMNPSPIPRFDIKKLNMSDNLFLFGAGREKRIYAIPPYTKV
                     EPLEFEDYKFEEEKFEGKYCSLCNSTNTFLDEVYDSDTNEKYYSCSDTSYCEKVRLKN
                     NSIDVTIGGTWNE"
     gene            16895..17761
                     /locus_tag="KRL67_001566"
     CDS             16895..17761
                     /locus_tag="KRL67_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBH1522453.1"
                     /translation="MSKINDSKRLESIDKQVLIGRNINLIYGDGCEKCFESTGYESNN
                     ICKYCGSIVACNNVNLELYEGEVLGIVGESGSGKSTLLKILFFQEHANSGEVYISHYN
                     KDTNILKLSDPKKRYIKNHFMGIVYQNPHLGLNLDFSSGGNIAEKLLMANLYNVEKIR
                     DRGKELLIKTNIPVERIDHKPKYFSGGMQQRVQIAKALSNNPPILLLDEVTTGLDVSV
                     QAEVLDLIREIQRELKISMIVVSHDFDVIKMLADRTIVMKNGSVIESGLTDQIMEDPQ
                     HPYTQQLINSLL"
     gene            17802..18515
                     /locus_tag="KRL67_001567"
     CDS             17802..18515
                     /locus_tag="KRL67_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBH1522454.1"
                     /translation="MKETILEVNNFSKDFELHALNKTITACSNISFEVSKGEFLGIIG
                     KSGAGKSTILKSIYKTYIPTTGEIIFNSEIYGNVDLAKIGDREMINLRKKEIGYVSQF
                     LKTLPRITAIELVVHSLIESGFEKDCSYDMAKDILNQFEIKENLWDAYPNNFSGGEKL
                     RLNLAQAMVKKPRLLLLDEPTASLDNQSKIYVKEKMLELKSQGTTMIGIFHDIEFMET
                     VIDKTFTMAKGTISESGVA"
     gene            18516..19685
                     /locus_tag="KRL67_001568"
     CDS             18516..19685
                     /locus_tag="KRL67_001568"
                     /EC_number="3.6.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate
                     5-triphosphate diphosphatase"
                     /protein_id="HBH1522455.1"
                     /translation="MIKLIHNANIVLEDMILENGHLIIEGDTIKKVSNDKIDYAFDFN
                     EIDEIIDAKNLYIIPGIIDIHSDAIEKEIEPRPSTLLPFNMAFYELDKKLPANGITTV
                     YHSISLGDGVGVRSIDNSLKMIKNIDSYKNIDSKSINHKVHLRYEVLYYEGLDKVLEL
                     LDENKIDYLSIMDHSPGQGQYTNPTFYREYATKVWGVTENYVDTWLDDLVNLHDNLDW
                     NKIANIIGIAKTKNINVASHDDDTLEKLDFIKNIGINVSEFPITLEVAKHSKKLGVLT
                     CLGAPNIVRGKSHNNNLKAMDAILEDCCDIVCSDYLPSAMIKSMCIVAERINNLNKAV
                     SLFTSNPAKAVGIYDERGSIKENKKADLVLVDIDSEYPKVVNTIVNGKTVYKREV"
     gene            19689..20525
                     /locus_tag="KRL67_001569"
     CDS             19689..20525
                     /locus_tag="KRL67_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HBH1522456.1"
                     /translation="MLTELHCHTSISDNNFTTEEIIKKAAEKNINILAITNHDTLSGL
                     DEALVLGEHYGVTIIPGIEISAYDYKNRKRVHILGYNIDLNSQEIKNLCNPMVCDRHK
                     ASVKMVNKIIDLGYKISLEDVKKYSSKTGIFKQHIMKALIDKGYTDNIYSSLYKELFH
                     RGNGKVYVSLKYVDYRDAIKAIKNSGGICVLAHPGQMDNFSAIEEMVKVGLDGIEVYH
                     PSHNKELEKESLFYAKKYNLVVTGGSDYHGFYSQHNYELGSKSLNKEDLFKFRLALGG
                     VL"
     gene            20526..21521
                     /locus_tag="KRL67_001570"
     CDS             20526..21521
                     /locus_tag="KRL67_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBH1522457.1"
                     /translation="MSFLQVKNVGKSYGQVKVLKDISIDIEKGEFICLLGPSGCGKST
                     LLRIIAGLEDKHGGKIIINDKDMTNSPPESRNFGIVFQSYALFPNMNVYKNIAFGLEN
                     KNISKSNIDKKVKEVLDVVELSGYEKKYPSQLSGGQQQRVALARAIALEPDFLLLDEP
                     LSALDAKVRLKLREQIRSLHRKLGITTIMVTHDQEEALCLADKMVVMNRGEIIQVGTP
                     KEVYKNPETPFVADFIGTINFIDDGINKIAIRPEDIKVESNRDSKDKDIKVGEILDIE
                     FRGFNYRITVEYRSKQMKLDVVSKVAEQMKLCIGSKINFKIPKEGIVQYKSEGCA"
     gene            21539..23227
                     /locus_tag="KRL67_001571"
     CDS             21539..23227
                     /locus_tag="KRL67_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative 2-aminoethylphosphonate ABC transporter
                     permease subunit"
                     /protein_id="HBH1522458.1"
                     /translation="MITSKNKRRISDSTIQKYFLIFSVIVLIVFILFPLVSLLKNAFM
                     DTSGNFIGISNFSKYIENPSLIASFKNSIFVSTVSSLISLCLAFIYSYAITRTNIKFK
                     NFFKTMGMFPLFAPTMLYGISLIYLFGNKGIFTTMGLEVPLYGPLGIIISQILFTFPQ
                     AFLILSVALSMADYRLYEAADSLGASEFKKFCKITIPGIKYSLLSSFFVSFILAFTDF
                     GAAKVVGGNYNVLATDIYKQVVGQFNIPMGATVSMVMLIPVLIAFTLDKMASKKQGMT
                     ITAKSMPYKIKENKFRDRVFLIVCSFITLLIFILIFVSVVGSVIKLWPYNLSFTMEHY
                     TFKGILGSGIKTYLSSLKISTLTAIFGTIFVFFSAYMIEKVDKFPKLRQTAYFLSMIP
                     MALPGLVLGISYITFFNSASNPLNFLYGSTAILVIVNIVHFYSVCFITSNSSLKMLDR
                     EYELVAKSINIPFYKVFFNITVPMSITSILEIVVYYFVNSMVTVSALIFLYTPQTQTA
                     SISILKLDEIGYIGPSAAMAVLVLLTNIIVRLLYEFVTKKLKNNTQKWQQKDVA"
     gene            23239..24306
                     /locus_tag="KRL67_001572"
     CDS             23239..24306
                     /locus_tag="KRL67_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative 2-aminoethylphosphonate ABC transporter
                     substrate-binding protein"
                     /protein_id="HBH1522459.1"
                     /translation="MKFKKLVSLGVLSTLLLSVITGCSTDSSGSDSKSDSKTKGDLTV
                     YTAIEEDSIEPYLATFKEKYPDIKLNIVRASTGDITARLLAEKDNPQADVVWGVAATS
                     LLVADDQGMLEPYAPKGSEEIESAFKDDKEVPSWVGIDAWMTGITVNTKELSDKNIPV
                     PSSYEDLIKPEYKGLISMPNPSSSGTGYLTVSALIQIMGEEKAWQYMDKLHENIGVYT
                     QSGSAPATSAASGEYPIGISFGYRGIKLKEEGYPVEVVFPKEGSGWDIEANALVKKDN
                     IKEASKVFLDWAISKDAMNEYSKNYAVTTISTGNPIPEGFPKKPLEQMIDNDLKSAAK
                     NREDILNKWISKYDGKTEKES"
BASE COUNT         9750 a         2757 c         4383 g         7594 t
ORIGIN      
        1 taactctaaa gaaactagtg ttactagatt ctaaaaaatt attaaaaact taatagaaat
       61 attaagcata aagattatgt ggttacaata gcagagagga tacacctgtt cccattccga
      121 acacagaagt taagctctct agcgctgatg gtacttggtg ggaaactgcc tgggagagta
      181 ggacgtagcc acgtaatttt tttatttttt gtcaaaaaaa acaaaaaata cgtatttctt
      241 ttttcatttc gggaaaacta aacacaggaa ttatggactt tataaaaatt taattcttga
      301 ggagtgatat aagtgaaaaa agttgcttta attttagcta taataggtgc acttaactgg
      361 ggtgcaattg gtattctagg tacagactta ataggtagta tctttggtgg aacatatgaa
      421 atggtaagta gaataatata cttcattgta ggtttagctg gattatactt aataccaagt
      481 ttaatgtctg atgatagaga ataatttatg cttttatgtt aaatacctca ttctaaatgt
      541 ttagaatgag gtatttaatt ttataaaaat attaatttgt ttctttttta atggtaaaat
      601 agataagaat tgaagtttta aaaagaagta ttatgtaaaa aaggagtact attaatatat
      661 gtttattatt aatgaattaa ataagattgt aaatgaagaa gtaatatctt ttcatatgcc
      721 tggtcataaa aagggacaaa tttataaaaa gttaggatat aaagatattc tagaaaattt
      781 gtataaaatg gatacaactg agatattagg aacagataat ttacactttc cagaaggcat
      841 tataaaaaat tcacaagaaa aagcatcaag agtatttaaa agtgattata cttgctattt
      901 agtaaatggt agtagctgtg gaatacaatc tgcaataatg tcagtatgta gacctaaaag
      961 taaaataata acaaatagag attgtcatca gtctgttata aatggatgta ttttaggaga
     1021 tatagatata gagtatgtgc cttgtgaggt aagtgaagag acaaatgtct taaaagggat
     1081 atctataagt aatgtgattg atatagtaga taaaaacttg gatgcaaaag cagtactgct
     1141 tacttatcct acatattatg gaatgacata tgatttagaa tacatatgta attatgcaca
     1201 tagcaaaaag atggtagtga tagtagatga agcacatggt gcgcatttag ggcttagtga
     1261 gaggcttcct aaaacagctc ttgaacaagg tgcagatata gttgtacaga gtacacataa
     1321 aactcttcca tcatttacac aatcgtcaat gatacatata cagggagacc gagttgatat
     1381 agataaaata ttgaacatgt taagaattac agaatcttca agcccttcat atttattatt
     1441 atcttctctg gaattagcag tagatatata tgaaaataaa ggaaaagaat taatggaaga
     1501 gcttttaaat aatattagaa ctttcaaaaa taatgttgat tcaaatatca atatatatga
     1561 cacaaatgat aaaacaaaaa tatttatatc atctaaaaat atgggtttaa caggttatga
     1621 actagaaagt attttaagaa acaaatataa catacaagtg gaattatcta attattatgg
     1681 agttctttta atatgtacaa taggaaatac tgctaaggat ttctctagtt tagaaacagc
     1741 attaaacaat gttataaagg aagactttaa atttgaaaag ttagaaaata aaaaataccc
     1801 tgttgttatt ccaaagaaaa tattaacacc tagagaagca ttttataaaa ctaaaaaaag
     1861 tgttaaaata tatgacagca taggtaaaat ttgtggtgaa tgtatagtgc catacccacc
     1921 tggaataagt gtaatatctc ctggtgaagt aataagtaaa gaaataatag actatctaat
     1981 gttttgtcat tctaaaggta tgattgtgag tggattgaaa gatgtagatt taaattttat
     2041 acaagtaata gatttagaag acatttaaaa attatgatat aaaagtagat agtttgaaat
     2101 tgtgaagaga aataaataga ctgcttatat agagctatct aataaaagta ctaaaaaagt
     2161 aaaaagtgtt tgtatggagg atatgaaaat gaaaggtaag ctaataatta tagagagtgg
     2221 ttcagatgca agtggaaagg ctactcagac agaaatatta tatgaaagat taaaaaaaga
     2281 aggctatatg attaaaaagg tagaatatcc aaattatgaa tctccatcat ctgccttggt
     2341 gaaaatgtac ctaaaaggag actttggtaa aaatgcatca gatgtagacc catatatagc
     2401 atcaactttt tttacagctg ataggtatgc atcatttaaa accgattggg aggagtttta
     2461 caatgaaggt ggaataatta tcgcagatag atatacaacc tctaatatgg tacatcaggc
     2521 ctctaaaatg gatatttgtg atagagaaga gtatttaaat tggttagctg attatgaatt
     2581 taatttattt aagataccaa aacctgattg tgttatattt ttagatgtgc ctattgaatt
     2641 tagtgaaaag cttatggaga atagaaaaaa taaaattaca ggggaagaaa aaaaggatat
     2701 tcatgaaagt gacatagagt atttagagaa atcttataac aatgctttat atatagcaga
     2761 taaatatgat tggaaaaaaa taaattgtgt tttagataac aaattaagaa gtattgaaag
     2821 tatacataat gaaatttata aaatagttct tgattcaata aaatctatgg agagataaat
     2881 atgtattttg aaaatattat agggcaagat tttgcaaaga aatatttaac aaattctata
     2941 aagaaaaata aacttaataa tgcgtatatg tttgaaggaa tggatggaat aggaaaaaag
     3001 aaatttgcag atgaattatc aaaactatta cttgattatg aaaatcttga aaatagtcca
     3061 gactatgtac tcataaaacc agatggaaat agcataaaaa tagctcaaat cagaaattta
     3121 caatcagata tagtaataag acctcataaa gactataaaa tatatataat caataatgct
     3181 gaaaaaatga ctgtagaagc ccaaaatgca cttttaaaga cgctagagga acctccaaac
     3241 tatgctataa taatcttagt cacaaacaac aaggaatctt tattagaaac tataaaatct
     3301 agatgtgata taataaagtt ctcgcctata ccaattgagg acttaaaacg ttatttaata
     3361 aatacaggta tagaagaaga aagagctcaa ttattggcta ttttttcgag aggaagtatt
     3421 gagaatgctc taaatctatc tcagtcctct gagttttcaa tgatgagaga agatatacaa
     3481 caatatattc agataatgtt agataaaaac atagtagaga ttttaaatat accaaataat
     3541 atggaaaaat atagaggtaa gattatagct ttattagata tgatgataaa ctattttcgg
     3601 gatatcatca tattaaaaga aaatgtaaac aagaacatgt taataaatgt agataagcta
     3661 gttttcattc aaaatatgag tggaaaaatt agttattctc aattgtccaa gattattgat
     3721 ataatagaag atgcaaagag caaaataaaa agcaattgca atttcaatat aagcatacaa
     3781 gttatgtctt taaatatata cgaggtgatt aaatgataaa gatagtaggt ataaggttta
     3841 aaagtgctgg gaaaatatat tattttgacc ctgttgattt taatatagag caagatatgg
     3901 atgtagttgt agaaactgca agaggcttgg agtatgggaa agtagtcgtt ggtccaaaag
     3961 atatggatga aagcgaatta gtttctcctt tgaagccaat tataagaata gctacagaag
     4021 aagataaaaa agtatatcta gaaaataagg aaaaggcaaa agaaactttt gagttatgcc
     4081 aacaaaaaat aaaagaacat gagttaacaa tgtttttaat agattgtgag tatacgtttg
     4141 ataggaataa gttgattttt tactttacag cagaaggaag aatagatttt agagaattgg
     4201 taaaagactt agctgctata tttaaaacta gaatagaact tagacaaata ggtgtcagag
     4261 atgaagctaa atctatagga ggtctagggc cttgtggtag aaaactttgt tgttcatctt
     4321 ggttaggtga ttttcaacca gtttcaataa aaatggcaaa agaccaaagt ctatctctaa
     4381 atccaacaaa aatatctgga atatgtggga gattattctg ttgtttaaaa tatgagcatg
     4441 atgtatatag tgaggcaata gaaaaaatgc ctgttgtagg tgcattggtg caatcagatg
     4501 atggaaaagg aaaagttgta gaagtaaatc ctttattaga acaaattaaa gtggaatttc
     4561 aagacaagac agtaaagata tacacaagag aagatataaa aatacttaga gagcctaaaa
     4621 aatgtgatgg ctgtggtggt aaatgtaagg ataatgatgg attagatgaa gccacactaa
     4681 gagagctaaa gaaattagaa gattaaattt aaggtagggc ttttcctacc ttttttaaag
     4741 caattaatgc ttaaagttat taatatttga ggagaattta tatatgcaat taaaagattc
     4801 agaaagaata gatgatttac aattaaaagg tcttaaaatt atacaagata ctaatggttt
     4861 ttgttttggt atagacgcag ttttattagc taattttact aaaataaaaa aagatgctaa
     4921 agtagtagat ttgggtactg gaactggtat tatacctatt ttgatagctg gaaaaagtga
     4981 agctaaaaaa attattggtg tagaaataca agaagatgta tatgaaatgg caactcgttc
     5041 tgtaaaatta aatggattag aagaaagagt taagattata aatgaagata taaaatacat
     5101 agacaaagtc ttagatgtaa atggctacca tgtagtcaca tcaaatcctc catatatgca
     5161 tattgacgga ataaaaaatc caaatgataa aaaagcaata tcaagacatg aagtaaggtg
     5221 taatttggaa gatgtaatta gagctgcatc cagattggtt atgccgagag gaaaattctt
     5281 catgatacat agaccaacaa gattggttga tataataact ttagggagaa aatataaatt
     5341 agagccaaag gttattcaat ttgtgcatcc aagacctcaa aaagcaccga acttagtttt
     5401 ggttcaattt gtaaaagatg gaagaccaga gcttaaaata ttggacccat tgtatgttta
     5461 tggagaagat gggaattata caaaagaatt aaaagcaata tataataata aagatatagg
     5521 agagttgtaa atgagtggga aattgtacat atgtccaaca ccaataggaa acctagaaga
     5581 tataacatat agaactttaa gaatattaaa agaagtagac ttgattgcag cagaagatac
     5641 aagacatagt ataaaacttt taaatcattt tgaaatctca aaaccactaa ctagttatca
     5701 tgaacataat aaggattcaa agggagatta tttgataaat aaactaattg atggtgaaaa
     5761 tatagctctg ataagtgatg caggtatgcc tggtatatct gacccaggag aagagataat
     5821 aaaacaagct atacaaaata acatagagat agaagtattg ccaggtgcaa cagcatttgt
     5881 aacggcttta gttggctctg gtatggacac acataaattt gtttttgagg gctttttaga
     5941 tagagataaa aaagttagga aagcacaatt ggaagaatta aaggaagaaa gtagaactat
     6001 aattttctat gaatcaccac atagattgaa ggatacacta aaagatatgc ttaaaattct
     6061 tggtaatagg aatatttcta taaatagaga acttacaaaa aaatatcaag aaataataag
     6121 agaaaatata gaaactacca taaaaatatt tgaggaaaaa gaagtaagag gagagtttgt
     6181 attaatagta gagggttttc atggtgagaa gtcaaagaaa gaaaattatg atgggcttac
     6241 agatagggaa tatgttttaa aactaattga aagtggtatg aacaaaaaag atgcaataaa
     6301 aatagtttgt aaagatagaa agttaaagaa agatgtggtt tataaacaag ttttagattt
     6361 ataaaacctg aatctgaggt gattttatgg aaataggaaa aattataata gagagtttaa
     6421 agagggaaga tacacataaa atatttatag aattagataa taagcatgaa ttagaaaaaa
     6481 taattcctaa aataagctgt atgaagtctg taggtgaatg taaatatcat gtagtaaatt
     6541 gttttgaaca ttctataaat gcattaaaag aattagaaat agtattaaat gataaggatt
     6601 tttttccaat acatctaaga aaacatgtgt caaactattt aaacacatat actgaaaaag
     6661 gaataaataa gttacatgta ttaaagctgg gaacattttt acatgatata ggtaagcctg
     6721 acagtatgac tttagatgaa acaggaagag ttcacttcac aaatcatgaa aaaataggtg
     6781 ctcaaataat tgacaatatg gggataaagt tggagttatc aacagagaca tatagactta
     6841 taagtaaata tgtaagatat catatgatac ttttgtctct gtataagaaa aatgatttga
     6901 gtaggaaaga attaattaat gtatttaact tggtagatga ggacactata ggtgtaataa
     6961 tgcttgggta tgcagatata gtgtctacag taaaattatt gggcaagagt ggagaagtga
     7021 gtgtattaaa gacatatatg gaatatattt taacaaatta tttgtataag agtaattata
     7081 cacatgtata aatttagtta atataattga gttttagatg attaattaaa gtctgaaata
     7141 cacggatttc aaaaacataa aagaagttgt ctttgttgta aaattatgaa agacaacttt
     7201 ttttatatca aaaattgaaa ttttattaag gatacttaat ggtctattaa ttaataaacc
     7261 attagtattt aatttatatt acaatattga ttttgcttgt attggcgttg atagtataac
     7321 agaggtctta gtagaaccaa ttttctttat agtatcaatc acattttcta gctcgtacat
     7381 atcttttaca attactttta aaagtaagca atcatcacct gttatatggt gacattctac
     7441 aatcctaggg tcctttgcag ctgactcaat aaattctgta tatccattgc ttggaagaga
     7501 aatatgaata aatgccttta taactctgcc taatgaatct gggttgacaa tagctttata
     7561 tccttctata acaccagact cttctaatct tttgactctt tctgaaactg caggagaagt
     7621 taaaccaact atttttccta agtccttcat agaaattctc ccatcatcct gtaaaatttc
     7681 tatgattctg taatctgtaa catccataaa taaacagtcc cccttaaata aaaataatta
     7741 atgctataaa acttttttag aatctatcat ttgtgttttt aaataccaaa gatttctaga
     7801 taaatcaacc ttataaatgt gtggttttga aagtcttttg tattgttccc agatagtact
     7861 taattcatat tgtacatgat tttttatttc aagaacagct tttttattat atttgcatct
     7921 gccttttata tacaatggtt taagaagctc tttaactttg taatttgtaa atacctttgt
     7981 tttccatgta taagtaggat ggaagatttc taaaggttta gactcatcta taacttcatc
     8041 atgtaacata atcaaatctg cctctgcctt attgtcttcg ttatatattc ttataacctt
     8101 cttgtaacca ggattattta ttttttctgg attctcagaa atctttatct taggttcaag
     8161 aactcctttt tcacaagatg cagctaattt gtaaactcct ccaagagaag gtgaatcgaa
     8221 agatgtaatg agctttgtac ctactcccca tgaatttata gtagctcctt ctgctttaag
     8281 tgatgttatg gtatattcgt ctaggtcatt agatgcagtg atgctgatat ctgaaaagcc
     8341 agcgttatct aattgttttt tagcttcttt agataaataa gctaaatctc cagagtcaag
     8401 tcttataccc ataggtttgt atcctttttc agaaatattt ttgaatactt ttattgcatt
     8461 aggaacccca ctgtttaata catcataagt atctacaagt agtagacatt tatctggata
     8521 aacatcagca tatgcttgga atgcttcaag ttcattatca aatttttgta cccaactgtg
     8581 agcttgtgta ccaattatag gtatatcaaa cattttccct gctagtacgt ttgcagttcc
     8641 agcacaacca ccaactacag cagccctagc tccatatatc cctgcatctg gaccttgtgc
     8701 acggcgtaat ccaaattcaa atacagggtc tccttgagct gcaaagcaaa ctctagaggc
     8761 tttagtagca ataagggact gaaaattaac tattgttaac aatgctgttt caataagttg
     8821 agcttgatat aaaggtgcct taacagtaat taatggctca ttagggaaca tgatagttcc
     8881 ttcctcaaca gcatatatat ctcctgtgaa tttaaattct tttaaaaatt ttaagaactt
     8941 gtcagaaaat aaatttaagt tttttaagta ctctaaatct tcatctgaaa aatgaagatt
     9001 atctatgtat tcaacaacct gatcaatacc acaaactata gtataccctc catcacaagc
     9061 gttttttcta aaaaacatat caaatacaac aatgtcttca tgaatattct tttcaaaata
     9121 gccatttaac atagtaagct gatataaatc agtaagaagt gttagatttc tcattatttc
     9181 tctccttaat tattattaaa tattaaaaat tgtaaaaact cccacactta tactaaaaca
     9241 taatatagta caactttcaa gtataaagta ttacctaact atcataaaat tatttaaaga
     9301 ggaggatgat tataatagga aaaatatggt tttatatggt atcgatagga ataataggaa
     9361 gcttgatttc taaaaatatg ggagaattaa ataaagttat attaagtgaa acatcaaaag
     9421 gcatagaatt tgcaataagt ctagctggta tcatggcact ttggatgggg ataatgaata
     9481 tcgcaaaaga gtcaggctta attgaaaaga taggtcaaaa attaaatcct ataatgagaa
     9541 gattatttcc ttctattcca aagggtcata aagcaatgtc ttatatagtt atgaatattg
     9601 ctttaaatat gttgggtgct ggaaatggtg caacagcatt tggtttaaaa gcaatgagtg
     9661 aacttcaaac tttgaataac aagaaagata cagccactaa tgatatgatt atgtttatgg
     9721 taatcaatat atcatctata caaataattc catttactat aatcaaatta aggatggata
     9781 tgggggctca aaatccaagt gaaataattt ttacgacttt atttgcaacc ataatatcta
     9841 cagcagtagc aataataaca tgtaaatttt ttcaaagaag atatagataa aggggatgta
     9901 gatacatgga gatattttca aacttactta taccaataat aattctgtac atagtagttt
     9961 atggtaaata taaaaaaata gatgtgtatg atagttttgt aaaaggagcg attgatggat
    10021 taaaagcagc atgggatatc ctaccatata taataggtat atttttggca ataggaatat
    10081 tcaaaactgg taaaggtctt gatatgttag aatggttatt tacaccaata gcaaatatgt
    10141 tgagtatacc aaaagaatta ataggtctaa taagtgtaaa acctttatct ggaagtggtg
    10201 ctttagggat gtacagtgaa cttgcaaata gagttggaat aggaagttta gtagaaaaaa
    10261 tgggtgccac gattgtaggt tcttctgaga ctatttttta cacaatggca atatactatg
    10321 gaagtcttaa gataaaaaat actagacata cattatcatg tgctatgatt agccatgttg
    10381 caggtgtcat agcagctgtt tttatttgct atgtaatatt tgtataattt gttgacaata
    10441 tgggaaaaat tttataatat agtgattata tagaaacaaa tagtaataca aattataagc
    10501 tatgaaaaga agagtaaata aaggtttttt ctatcagaga gctaggttag gtgaaagcta
    10561 gtgtcaaaag ctttattgaa gatggtcttg gagcttttta tctgagcaag tgtgctaggg
    10621 taaaacggtt ggacacgtta tagtctacta aggtatctat aaaatgtttc tacatttcat
    10681 gtgaaacata actattatag atgaattagg gtggtaccgt gaataatctc gcccctatgt
    10741 taactcatag gaggcgattt tttattattt aaatgaaaga atcatatgat tacgaacgat
    10801 aaaaaattag gaggtaaata acatgagcaa accgagtttt tatgtaacaa caccaatata
    10861 ctatccaagt ggaggattac acataggaca tacttattca acagtagccg cagatacaat
    10921 agctagattc aagcgtttct gtggatatga tgtaaagttc ttaacaggaa cagatgagca
    10981 tggtgaaaaa attcaaaaga aagccataga acaaggaatg tcagagatag agtatcttga
    11041 tggaatgata aaagatataa aggccttatg gaatactatg gatatatctt atgatgattt
    11101 tataagaact acagaaaaaa gacatacaga cataatacaa aagatattta ctaaactata
    11161 tgagcaaggt gatatatata aaggtgaata tgaaggtaga tactgtactc cttgtgagag
    11221 tttctggaca gaatctcaat tactagaagg aaataaatgc cctgactgtg ggagagaaac
    11281 atatttagta aaagaagaat cttatttctt tagattatct aaatatgaag atagattgaa
    11341 agagttattt aaagatgata gtttctgttt tcctgctgct agaaaaaatg aaatggtagc
    11401 aaacttttta gacaaaggat tagaagattt aagcgtaact agaacgactt ttgactgggg
    11461 tataaaagtt ccttttgatg aaaaacatgt aatttatgtg tgggtagatg ctttatgtaa
    11521 ctatataaca gcattaggtt atatgactga taatgatgaa gaatttagaa aatattggcc
    11581 tgccaatgtt caaatagtag gtaaagaaat agtgagattc catacaataa tatggccagc
    11641 acttttaatg gctttaggtc tagaagttcc aaaacaagta tttggacatg gatggatact
    11701 gtttgctgat gataaaatga gtaaatcaaa gggtaatgtg gtttatccag aacctataat
    11761 agaaagatat ggaatagaca cacttaagta cttcttatta agagagtttg catttgggca
    11821 agatggaagt tatactcata gaaattttgt aacaagaata aattatgatt tagcaaatga
    11881 tttaggaaac ttaataagta gaactgtagc tatggttgaa aagtacaata atggtataat
    11941 accaacagca aaagtttcaa ctgattttga tgctgactta aaagagcaag ccgtttcaac
    12001 tagagaaaac tttgaagctg aaatggataa aatgcaattc catgaagcat tagagagtgt
    12061 atggaaatta gttagaagaa ctaacaaata tatagatgaa actatgcctt gggcactagc
    12121 taaagatgaa acgaagaaag atgaattaga cacagtttta tataatttat gtgaatctat
    12181 aagaataatc gcaacactaa taaatcctat aatgaatgag actgctaata aaatatatga
    12241 acatatagga ataaaaggtc aagatgatat aactacatgg gaaagtacaa aaacatttgg
    12301 tcttattgga gaaaatgtaa aagtatttaa aggtgaacct ttattcccaa gattagatgt
    12361 agaaaaagag atagaagaat taactaaaat gttctctggg aaacctccag tagaagaaaa
    12421 acctttagag cataaagaag aaattactat tgatgattta gacaaaatag agcttagagt
    12481 agggaaaata ataagctgtg aaaaacatcc aaaagcaaat aaattattag tatcacaagt
    12541 taagattgga ccagaaacaa gacaaatagt atcaggaatc gctgaatact ataagccaga
    12601 ggatttagta ggaaaagagg ttacagtggt atgtaactta aaaccagtta aattaagagg
    12661 tgtagaatct caaggtatga tattagcagc aggtgatgat ggagacccat atgttttacc
    12721 atttacacaa ggggctaaag atggatgcga ggttcgttag taggtaatta aatagtaagg
    12781 agattaaaga tgttatttga ctcacatgca catttaaatg atgaaagttt tgatgaagat
    12841 agagatgaac ttattggttc attaaaagat aaaggtgtag atttggttgt aaatccagga
    12901 gcagatatag agacgtcgat aacagcaatt gaacttgcga aaaaatacga ctttatatat
    12961 tcagcagttg gggtacatcc acatgatgta tcaaaactag atgatacagc aatagaaact
    13021 ctaagaaaac ttgcaactga gaatgaaaaa gttgtagcta ttggagagat tggattagat
    13081 tattattatg attattctcc aagagaagaa caaaaagagt ggtttaagaa gcaaattgaa
    13141 ttagctaatg agctaaaact tccaataata atacatgata gagatgcaca tggagatact
    13201 tttgagataa taaagaatac taaaaatcct gaaataggat gtgtacttca ttgttatagt
    13261 gggaatgtag agttagctag agaatatgtt aagatgggtt gttatatatc aattccaggc
    13321 acggttactt ttaagaataa taaaaaaact agagaagtag taagagaaat acctctagaa
    13381 agattattca tagagactga ttcaccatat atgtcaccag agccacatag aggaaagaga
    13441 aacaatcctt ctcaagtatc ttttgttgct gataagatag ctcaggaaaa agggatatct
    13501 tatgaagaag tatgtagagc tacaaaagaa aatgcaaaga aattttttaa tataaaatag
    13561 ataaattaaa tcttataata atatatataa taagtaatta taagtatagt atgagagtgc
    13621 ctcaaaatga gttttttagt tcatgaggca ctcttttttg tatgaaatca aatatatttt
    13681 catcatttca acaatgaaaa agaagtttat catattttaa gacagtctat ttttgttttt
    13741 atttatttaa ttcatatttt aaaatatatt ttcttaacat tgttaaaaaa gaatttacaa
    13801 gatattcatt agaattaact caactctaat attcatttaa cttaggttgt ttattatatt
    13861 attaaagaga agtaaacgaa aggtagatta aggaggctaa attttgtata cagttgatag
    13921 tactaagata ttgataaata tgaacttaga gaaactagag aagatatact caatgataaa
    13981 agatacatca agtatttcta taattaaaga acctaactta gctacagtaa tggtaagagc
    14041 taatgaaagt gtaaaaaaca caacatttaa tttaggagag atattagtta ctgaatgtag
    14101 tgtaaaggta gatgaaagct tgggttacgg aatagtttca gaaaacaata ataagaaagc
    14161 catttactta gcagttatag atgcagtttt acatagcaat aatagtaaat ttgatgagtt
    14221 aaagaattac ataaataaaa gtgtatatga agagaactta aggtatgaac aagagattat
    14281 tgatgaattt agcttgatta ataaaacgaa agttcaattc aatactatgg attaaatagg
    14341 aggagaatca tggtaaatta tacacaagaa atatatagaa agatgctaga tagtgcttca
    14401 tttcctggga aattaaataa tatagataat ataaatatag aaaataatac aaaactattg
    14461 aatggagcaa ttggtatagc aatcacttta ttagaccaag aagttacctt ctatatagaa
    14521 aattatactc aagaagatat aaaaaatata agagcactta caatgtcaaa tcaaatagat
    14581 tatagaaaat cagattatat atttttagat attaattcag aagtggatat attacagatt
    14641 aaaataggtt cttttgaata tcctgatgaa agtgcaacta tagtacatca agtaaatgat
    14701 ttatcaacgt actctcaaac taagtatata aaacttgatt tgagtggtcc tggtataaaa
    14761 tctaaaaata gtatatatat aaatggagtt agagaagaat ttatagaaag attatctacc
    14821 ataaataaag attatccaat aggaatagat ttgattttag tagacaaaaa aggagaactt
    14881 gcatttatac caaggtcatc aaaattaagt tgggaggtat tgtagaaatg gcttatgtag
    14941 cagttaaagg tggagaagaa gccatattac aggctaaaaa tatacttgag ttttataggg
    15001 taaaaggaga tagcttgcct atagatgtaa agcagattga ggaacaaatg tcttatgctg
    15061 tagacaaagt aatgtctgaa ggaagtttat acaacaagga actatcagca ttggctataa
    15121 aacaagctca aggtgatata ttagaagcct ctttctatgt aagagcttta aggtcaacaa
    15181 tgcctagaat tacatattca gaacctataa atacagaaga gatgagaata aaaagaagaa
    15241 tatcagctac ttttaaagat ataccaggag gtcagtatct tggggcaact agagactatg
    15301 aaaatagaat attaaatgta gacttattag aagaagatga aaaaaagaat agagacttta
    15361 aagatagttt tataaatgaa ttagaaacag tagatgaaat ccctgaattt acaaaaatat
    15421 ctaatatatt tagaaacgaa aatttattac aagaggaagt ttgtgaaaac aaatcggaag
    15481 aagttattga tgatattaca agaaatagtt taagatttcc agtttctagg tcagcaaagt
    15541 tacaggcatt aactagaggt gaaagtggag tagtgtcagg ttttgcttat gctattgtaa
    15601 gaggttttgg tgatgaacat ccatatataa gtgaacttag aactggtaat gtatttataa
    15661 aagtaaaaca tcccatctat gaagatgaag aaataataat aggtgatatg ctggttactg
    15721 aatgtgaaat agtttcaaaa gcatcacaaa ataagactga aaattctaaa actaattcag
    15781 aagataaaaa taagatgtct ttaggatatg gtctttgtat ggggaataat gagaataagg
    15841 ctatatctat ggccatacta gacaaatctt tggagccaaa tagcgaaaaa ctagcacaag
    15901 atgaagaatt tgtattgctt catatagatg gaatagattc attaggattt atatctcact
    15961 ttaagcttcc tcactatgta gattttaaag cggatgtaga aagaataaaa tagtatatgg
    16021 aggtaagttt aggtgatata caactatggt tttttagatg aaaatacaaa gaaggaaata
    16081 agaagaaaaa tattaaaagc agtttcaata cctggttatc aagttccctt tggctcaaga
    16141 gaattgccta tagctaaagg atggggtact ggagggttac aattaacact atcattaatt
    16201 ggtaaaaatg atgtagtaaa agtaattgac cagggaagtg atgattctac aaatgcttgt
    16261 aatataagga actttattgc aagtgtaagt aatgtagaaa cgacaaaaga tacgttagaa
    16321 gctacattga ttcaaacgag acatagaata ccagaagaaa aacttaaatc taatcaaata
    16381 ctaatatttc aagtaccaat accagaaacc cttagaatag tggaacctag tgaagtagaa
    16441 acaagaagaa tgcattcaga agaagattat agtaggatgt gggtatatct ttatgaagat
    16501 atagttagat ttaatgatat atctatagca gttgaatatc catgtaaagt aaatgataga
    16561 tatttaatga atccatcacc tatacctaga tttgatataa aaaaattaaa tatgtcagac
    16621 aatttattct tgtttggtgc aggtagagaa aaaagaattt atgctattcc tccatataca
    16681 aaggtagaac ctttagagtt tgaggattat aagtttgaag aggaaaaatt tgaaggaaaa
    16741 tactgtagtt tatgtaacag tacaaataca tttttagatg aagtatatga cagtgatact
    16801 aatgaaaagt actatagttg ttcagataca agttattgtg aaaaagttag attaaaaaat
    16861 aacagtatag atgtgacaat aggaggtact tggaatgagt aaaataaatg actctaaaag
    16921 attagaaagt atagacaagc aagtattaat aggaagaaat atcaatctta tttatggaga
    16981 tgggtgtgaa aagtgttttg aaagcacagg gtatgaaagc aataatatat gtaagtattg
    17041 tggttctata gttgcatgta ataatgtaaa tttagaactt tatgaaggag aagtccttgg
    17101 aattgtaggt gaatctggtt caggaaaaag cactttatta aaaatactgt tttttcaaga
    17161 acatgcaaat agtggagaag tatatatatc ccattacaat aaagacacaa atatactaaa
    17221 gctttcagac ccaaaaaaga gatacataaa gaatcatttt atgggtattg tgtatcaaaa
    17281 tccccacctt ggtttaaatt tagactttag tagtggtggt aatatagcag aaaaattatt
    17341 gatggcaaat ttatataatg ttgaaaaaat aagagataga ggcaaagaac ttttaataaa
    17401 aactaatata cctgtagaaa gaatagacca taaaccaaaa tactttagtg ggggaatgca
    17461 acaacgagtt caaatagcaa aagctctttc aaataaccct ccaatattgc tattagatga
    17521 ggttacaaca ggtttagatg tttctgtgca agcggaagta ctggatttaa taagagaaat
    17581 ccaaagagaa cttaagattt ctatgatagt tgtatcccat gactttgacg ttataaagat
    17641 gcttgctgat aggacaattg taatgaaaaa tggaagtgtg attgagtctg gtcttacaga
    17701 ccaaataatg gaggacccac aacacccata tactcagcaa cttataaatt cattgctata
    17761 aatatcgttt gtaaataagc aaaatggggg tttttaataa aatgaaagaa actatacttg
    17821 aagtaaataa ttttagtaaa gactttgagt tacatgcatt gaacaagact ataacggcat
    17881 gtagtaatat aagttttgaa gtttcaaaag gtgagttttt aggaataata ggtaaatctg
    17941 gagcaggtaa gtctaccata ttaaaaagta tctataaaac atatatacca acaactggag
    18001 aaataatttt taattcagaa atatatggta atgtagacct ggctaaaatt ggagaccgag
    18061 aaatgataaa tcttagaaag aaagaaatag gatatgtatc acagttttta aagacacttc
    18121 cacgaataac tgcaattgaa ctagtagtac attctttaat tgagagtggg tttgaaaaag
    18181 attgttcgta tgatatggca aaagatatac ttaatcaatt tgaaataaag gaaaatttat
    18241 gggatgctta tccaaataat ttttctggtg gagaaaaatt aagattaaat ttagctcaag
    18301 ctatggttaa aaagcctaga cttttattac ttgatgaacc aactgcatca cttgataatc
    18361 aatctaaaat atatgtaaaa gaaaaaatgt tggaacttaa aagtcaagga actacaatga
    18421 taggaatttt tcatgatata gagtttatgg aaactgttat agataagacc tttactatgg
    18481 caaaagggac tataagtgaa agtggggtag cctagatgat aaaactaatt cataatgcaa
    18541 atattgtatt agaagatatg atattagaaa atggccattt aatcatagag ggagatacaa
    18601 taaaaaaagt atcaaatgat aagattgatt acgcatttga ctttaatgag atagatgaaa
    18661 taatagatgc aaaaaactta tatataatac ctggtattat agatattcac agtgatgcca
    18721 ttgaaaagga aatagagcca agaccaagta cattattacc atttaatatg gcattttatg
    18781 agctagataa aaaactacct gcaaacggaa taactacagt ttatcattct atatccttag
    18841 gggatggagt aggagttaga agtattgata actctttaaa gatgattaaa aatattgatt
    18901 catataaaaa tatagatagt aagagtataa atcataaagt tcatttaaga tatgaagtgt
    18961 tgtattatga aggtttagat aaggttttag aactattaga tgaaaataaa atagattact
    19021 tatcaataat ggaccattct ccgggacaag gtcagtatac aaatccaaca ttttatagag
    19081 agtatgctac taaagtgtgg ggagttacag aaaattatgt agatacatgg ttagatgatt
    19141 tagtaaatct gcatgataat ttagattgga ataaaatagc taatattata ggtatagcca
    19201 aaactaaaaa tataaatgtt gcatcacatg atgatgatac attagaaaaa ctggatttca
    19261 taaaaaatat aggcataaat gtatctgaat ttcctataac tcttgaagtt gcaaaacatt
    19321 caaagaaatt gggtgttcta acttgtttag gtgcaccgaa tattgtaaga ggtaaatctc
    19381 ataataacaa tttaaaagca atggatgcta tattggaaga ttgttgtgac attgtgtgtt
    19441 cagattatct tccatcagct atgataaagt ctatgtgtat agttgcagaa agaataaata
    19501 atttaaataa agcagtcagt ttatttacct caaaccctgc taaagcagtt ggtatatatg
    19561 atgaaagagg tagtataaaa gaaaataaaa aagcagattt agttttagta gatattgata
    19621 gtgaatatcc aaaggttgta aatactattg taaacggaaa aacagtttat aaaagggagg
    19681 tataaagatt gttaacagag ttacactgtc atacaagtat atcagataat aattttacta
    19741 ctgaagaaat aataaaaaaa gcagcagaaa agaatataaa tatcttagca ataacaaatc
    19801 atgacacatt atctggattg gatgaagctc ttgtgcttgg agaacattat ggagtaacaa
    19861 taataccagg tatagaaatt tcagcttatg attataaaaa tagaaaaaga gttcatattc
    19921 ttggatataa catagattta aacagtcaag aaataaaaaa tttgtgcaat ccaatggtat
    19981 gtgacagaca taaagcatct gttaagatgg taaataaaat tatagattta gggtacaaaa
    20041 tatctctgga ggatgtaaag aaatactcat caaagactgg aatatttaaa caacatataa
    20101 tgaaggctct tatagataaa ggatatacag ataatatata ttcaagtcta tataaagaat
    20161 tgtttcatag aggaaacggc aaggtatatg tatctttaaa atatgttgat tacagagatg
    20221 caataaaagc tataaaaaat tctggtggaa tttgtgtgtt agctcaccca ggtcaaatgg
    20281 ataattttag cgctattgaa gagatggtaa aagtaggatt agatggaata gaagtatatc
    20341 atccatctca caacaaagaa ctagaaaaag aatcgctttt ttatgctaag aagtacaatc
    20401 tagtggtcac aggaggctca gattaccacg gattttattc tcaacataat tatgaacttg
    20461 gaagcaagag cttaaataaa gaagacttat ttaagtttag gttagcttta ggaggggtat
    20521 tataaatgtc ttttttacaa gtaaaaaatg taggtaaaag ttatggacaa gttaaggtgt
    20581 taaaagacat atctattgat attgaaaaag gggagtttat ttgcttatta ggtccaagtg
    20641 gttgtggaaa aagtacactg cttagaataa ttgcagggct tgaggataaa catggtggaa
    20701 aaataataat aaatgataaa gatatgacta attcaccacc tgaaagtaga aattttggta
    20761 tagtttttca atcatatgca ttatttccaa atatgaatgt ttacaaaaac atagcttttg
    20821 gactagaaaa taaaaacata agtaaatcta atattgataa aaaagttaaa gaagttctag
    20881 acgttgtaga attgagtgga tatgagaaaa aatatccatc acaattatca ggtggacaac
    20941 aacaaagggt tgcattagct cgtgcgattg ctttagagcc agacttttta cttcttgatg
    21001 aacctttatc tgcattagat gcaaaagtaa gacttaaact tagggagcag attagaagtt
    21061 tacatagaaa actaggcatt acaactataa tggtaacaca tgaccaagaa gaagctcttt
    21121 gtttagctga taagatggtt gttatgaata gaggagaaat aatacaagtt ggaacgccaa
    21181 aggaggtata taaaaatcca gaaacacctt ttgtagctga ttttatagga acaataaatt
    21241 ttatagatga tggtattaat aagatagcta ttagaccaga agatataaaa gtagagtcca
    21301 atagagattc taaagataag gatataaaag tgggagaaat tttagatatc gaatttagag
    21361 gatttaacta tagaattaca gttgagtatc gttctaagca aatgaaatta gatgtagttt
    21421 ccaaagttgc agaacaaatg aaactgtgca taggttctaa aataaacttt aagataccta
    21481 aagaaggaat tgtacaatat aaatcagagg gatgtgctta atagctaggg gtgaataaat
    21541 gataacatca aaaaataaaa gaagaataag tgatagcaca atacaaaaat atttcttgat
    21601 tttttcggta attgtactaa tagtatttat attatttcca ttagtatcat tattaaaaaa
    21661 tgcatttatg gatacaagtg gaaattttat aggaatttca aactttagta aatatattga
    21721 aaatccaagt cttatagcat catttaaaaa ttcaattttt gtatctacag tctcatcttt
    21781 aatatcacta tgtttagcat ttatatattc atacgcaata acaagaacaa acataaagtt
    21841 taaaaatttt tttaaaacta tgggaatgtt tccacttttt gcaccaacta tgctatatgg
    21901 tatttcactt atataccttt ttggaaataa gggaatcttt acaacaatgg gtttggaagt
    21961 accactatat ggtccattag gaataattat ttctcagatt ctatttacat ttccacaggc
    22021 ctttttaata ttatctgtag cactttcgat ggctgattac agactttatg aagcagcaga
    22081 tagcttgggc gctagtgaat ttaagaaatt ttgcaaaata acaattcctg gtataaagta
    22141 ctcgttactt agttcattct ttgtatcctt tatacttgca tttacagatt ttggagcagc
    22201 taaggtagtc ggaggaaact acaatgtact ggccactgac atatataaac aagtagttgg
    22261 acaatttaac ataccaatgg gagctactgt ctctatggta atgttaattc ctgtactaat
    22321 agcatttaca ttagataaga tggcaagtaa gaaacaaggt atgaccataa ctgcaaagtc
    22381 tatgccatat aagattaaag aaaataagtt tagagataga gtatttttaa tagtatgtag
    22441 ttttataaca ttgctaattt ttattttaat attcgtttca gttgtaggtt cagttataaa
    22501 gttatggcca tataatttaa gctttacaat ggaacactat acatttaaag gtattttggg
    22561 aagtggaata aaaacatact taagcagttt gaaaatatca actttaacag caatatttgg
    22621 aactatattt gtatttttta gtgcttatat gatagaaaaa gtggataagt ttcctaaatt
    22681 aagacaaaca gcttactttt tatcaatgat accaatggcc ttacctggct tagttttagg
    22741 tatatcatat ataacttttt tcaattcagc aagtaatcca ttaaattttt tatatggaag
    22801 tacagcaata ttagttatag taaatatagt tcatttttat tcagtttgtt ttataacttc
    22861 aaattcatct ttaaaaatgt tggatagaga gtatgaatta gttgcaaaat ccataaatat
    22921 tccattctat aaagtatttt ttaatataac agttccaatg tctataacat caatacttga
    22981 aatagtagta tattattttg taaattcaat ggttactgtt tcagcattaa tatttttata
    23041 tacacctcaa actcagactg catcaatatc aatactaaag ttagatgaga tagggtatat
    23101 aggaccatca gctgctatgg cagtattagt gcttttaaca aatataatag taagactttt
    23161 gtacgaattt gtaacaaaga aattaaaaaa taacacacaa aaatggcagc aaaaagatgt
    23221 tgcatagggg aggtaaaaat gaaatttaag aagctagttt ctttaggagt attatcaaca
    23281 cttttattaa gtgtaataac aggatgtagt actgattcat caggttcaga ttcaaagtct
    23341 gatagtaaga caaaagggga tttaacagtt tacacagcaa ttgaagaaga tagtatagag
    23401 ccatatttag ctacatttaa agagaaatat ccagatatta aattaaatat tgttagggca
    23461 tcaacaggag atataacagc aagattatta gctgaaaagg ataatcctca agcagatgtt
    23521 gtatggggag ttgcagccac aagcttattg gtcgcagatg accagggcat gctagaacca
    23581 tatgctccaa aaggttctga agaaatagag tctgcattta aagatgataa agaagttcct
    23641 tcttgggttg ggatagatgc ttggatgaca ggtataacag taaacactaa agaattaagt
    23701 gataagaaca taccagttcc tagttcttac gaagatttaa taaagccaga atataaaggt
    23761 cttatatcta tgcctaatcc atcttcttct ggtacagggt acttaacagt atcagccctt
    23821 atacaaatta tgggagaaga aaaagcatgg cagtatatgg ataagttgca tgagaatatt
    23881 ggtgtataca ctcaatctgg ttcagctcca gctacatcag cagcttcagg agaatatcct
    23941 attggtatat cttttggata tagaggtata aaattaaaag aggaaggcta tccagtagaa
    24001 gtagtatttc caaaagaagg ttctggatgg gatatagaag caaatgctct tgtgaaaaaa
    24061 gataatataa aagaagcatc aaaagtattt ttagattggg ctataagtaa agatgcaatg
    24121 aacgaatact ctaagaacta tgcagttact actatttcta caggaaatcc aataccagaa
    24181 ggattcccta aaaagccatt agagcaaatg atagataatg atttaaaatc ggcagcaaaa
    24241 aatagagaag atatactaaa taaatggata tcaaaatatg atggtaagac agaaaaagag
    24301 agttaagtaa aactatacaa atggagtaaa aagaaagaat ttttttaagt agattttatt
    24361 ataataatat aagttgagaa taatataggt caagaaaagt gaatattcta taaaaatgta
    24421 tagagtaagt ataccattca gtttgaatgg tatatttttt atttttggag attaatttag
    24481 aaat
//
LOCUS       DAEPXF010000045        24292 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain HB27
            SAMN13376389-rid12479423.denovo.047, whole genome shotgun sequence.
ACCESSION   DAEPXF010000045 DAEPXF010000000
VERSION     DAEPXF010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13376389
            Sequence Read Archive: SRR10518432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 24292)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24292)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001069624.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001069624.1
            Genome Coverage       :: 315x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/15/2021 01:54:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,165
            CDSs (total)                      :: 4,076
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 89
            rRNAs                             :: 10, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 10 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 19 of 69
            Pseudo Genes (incomplete)         :: 44 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 10 of 69
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24292
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13376389-rid12479423.denovo.047"
                     /strain="HB27"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="China: Hebei"
                     /collection_date="2017"
     gene            9..2216
                     /locus_tag="KRL67_001573"
     CDS             9..2216
                     /locus_tag="KRL67_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HBH1522460.1"
                     /translation="MYNKYNNLYLLLGKENVNMDSYRQLKAKIPCKETGIEIKRTLCD
                     ICSPGVHCGIDAYVKDGKVIKIEGTKEHPMNKGLLCTKGLCNREYIYREDRIKTPLKR
                     IGKRGEGKFKPISWDEAYREIAEKLNRVKSEYGAKHVAFFTGYGKWYRPFLQRFASSF
                     GSPNYGTESSSCSTSAQMAGNVATGRNFTMQDMENAKTYLGWGFNPYYSKFLNSVNLI
                     KLKEKGLKFIIVDPRITPTTQKLADLHLRIKPGTDGALALGMANLFIQKDLIDREYIE
                     KHVHGFNEYKEYVKLFDLDTVSRITGVSKDNIVKAVKMLSQGPFCINESAAPIVHHQN
                     GFQNYRAMMSLSAITGNYDRIGGNIPKQDYFAGKIGGFQTLEKEFVKEVLPKNINERI
                     GGERFPLWNEMVTQFQAMDLPRQIEEKTPYEIRALFCHGINARMFPNSGRLFKALNSV
                     DFFVNIDLFMTDTSKYADILLPACTSFEREECKSYPGGYITYTKKVIDKLYDSKSDVE
                     ILSDLANVMNIDDDLLKAGYEASIRYMFKNTCVDVDKLKESDLPLKCKDFKPYIVGSY
                     TREGYDTSTSRFELKSTIIEKYKDFGLDALPTYRGFNDDIDNEYTHLLTSGARISNAL
                     HSRLHDIKLSRILKNEASCDISILDAKELDVISGDYIKITTKTGSLDVKVNITDRVLY
                     RNIHMYHGYREADVNSIVCDNFDPYSGFPAYRSCKCKIEKMNRQMEGYNEKFI"
     gene            2200..2622
                     /locus_tag="KRL67_001574"
     CDS             2200..2622
                     /locus_tag="KRL67_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HBH1522461.1"
                     /translation="MKSLFKFNKELCTACGACLLACIDQNDNDIDNGELFRCVFTIEE
                     GESVEYFSLACIHCDEPKCLDICPKDCFKKEEGFVVLDNQNCIGCKLCEKACEYGALI
                     YGNDGKANKCNGCIERYKCGMDSPCVMVCPTGALKFNK"
     gene            2796..3371
                     /locus_tag="KRL67_001575"
     CDS             2796..3371
                     /locus_tag="KRL67_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HBH1522462.1"
                     /translation="MVKVLAFMGSPRKKGNVDTILDEIIKGVNDNNVEVKKYYLNDMN
                     IKGCQGCLYCRKVPTCAFKDDMIDIYKDIKSAEYIIIGSPVYICQVSAQTKLLLDRLY
                     PLTEINKSKHTPRFGQKKLIMVYTQAAPVSFLFKKYFKYTSKHLKGMGLNHYKTIVAT
                     KAFERNSTKKNFKVLRKAYKLGMKIAIDING"
     gene            complement(3496..4212)
                     /locus_tag="KRL67_001576"
     CDS             complement(3496..4212)
                     /locus_tag="KRL67_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HBH1522463.1"
                     /translation="MQQGSDYLKKFLEKHIDLLKKIPLFEGIKSNELEEMLECLGVVD
                     KTYPKDSIIFSVGSEVTSIGIMLKGSAHIIKEDIEGNRNIVAELLPGDLFGEVFACTR
                     LHKSNVTVTTTSSCDVLFIKFKSVTGICSSTCVFHNRLVENMLQLIAEKNILLHNKIE
                     LLSRKTTREKLLMYFSKQIEQTGSHKFTIPFSRNEMADFLCVDRSSMSRELGKMRDEG
                     LLSFNKNKFEIFCFPNKLNI"
     gene            4377..5102
                     /locus_tag="KRL67_001577"
     CDS             4377..5102
                     /locus_tag="KRL67_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HBH1522464.1"
                     /translation="MMRKIIKIDEGKCNGCGLCVEACHEEAIGMVNGKAKLLRDDYCD
                     GLGDCLPTCPTNAISFEYREAAEYDEAAVKANMEAKKAQKKTLACGCPGSQSKSINRG
                     VSNSASISNDIVETKGSQLNQWPVQIKLVPTNASYLKNASLLIAADCTAYAYGNFHNK
                     FMKNKVTLIGCPKLDEVDYAKKLTEILKENDIKNIVVTRMEVPCCGGIVSAVKTALQN
                     SGKMIPWQIVTISVDGKIVDGEI"
     gene            5162..6754
                     /gene="hcp"
                     /locus_tag="KRL67_001578"
                     /gene_synonym="priS"
     CDS             5162..6754
                     /gene="hcp"
                     /locus_tag="KRL67_001578"
                     /gene_synonym="priS"
                     /EC_number="1.7.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxylamine reductase"
                     /protein_id="HBH1522465.1"
                     /translation="MEKMFCFQCEQTAGGKGCTINGVCGKKGGTANLQDELTGVLIGL
                     SRATIGNKNRPTNETDKIMIEGLFTTITNVSFDDEAIKRQIEKTEIEKSKLVPRCLDC
                     STTCNRNDNYDMKDLWNDNEDIRSLKSLILFGLRGMAAYAYHAMVLGYTDKDVNEFFY
                     EGLIAVGGDLSIDELLGLVMKTGEINLKCMELLDRANTETYGTPEPTQVSMKIEKGPF
                     IVVTGHDLYDLHKLLEQTKDKGINIYTHGELLPAHAYPKLKEYKHLKGNFGTAWQNQQ
                     KEFDNIPAPILFTTNCLMPVKDSYRDRVFTTEVVAYPGMVHIDEDKDFSSVIEKALEL
                     GGYKEDVHMTGINGGDTLTTGFARGTVSSVQDKVVDAVKSGAIRHFLLVGGCDGAKPG
                     RNYYTEFVKQAPKDTVILTLACGKYRFNDLDLGDIGGLPRLMDMGQCNDAYSAIKVAV
                     SLANAFECGVNDLPLSMVLSWYEQKAVCILLTLLHLGIKNIYIGPSLPAFLSKNVLDI
                     LVDKFALTPISTPEEDMKKILG"
     gene            7075..7980
                     /locus_tag="KRL67_001579"
     CDS             7075..7980
                     /locus_tag="KRL67_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HBH1522466.1"
                     /translation="MKNSNFNILAFDGGGLKGALSISILERIVKEYPNLLNNINMFGG
                     TSTGSLIALGLAYGVSPKEIKELYSIENSKYIFNKSYAEILRPKYENKNLKEVLLSIF
                     PEELELKDLNKLVMIPSFYIGNEENAWKPVFYNNMPNSFTKTSKVVDVAMASSAAPVF
                     FPTYNHHVDGGIIATDPSLACIIHAIDSGFKLKNTRLFSIGTGYVYNSIKADTTEWGA
                     IDWIINKEPDLPIISITLEGNSQMSQLFSQKLLGDNYYRLNPKMEKDVAMDDCDALEY
                     LMSLGENCDIKDAFGWIKKKSFYRN"
     gene            8104..9057
                     /gene="msrA"
                     /locus_tag="KRL67_001580"
     CDS             8104..9057
                     /gene="msrA"
                     /locus_tag="KRL67_001580"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HBH1522467.1"
                     /translation="MIKLATFAGGCFWCMVKPFDKYDGVIKVVSGYTGGHLENPTYQE
                     VCSGTTGHLEAIQITYDDDIISYDELLNIFWKQINPTDDGGQFGDRGTQYKTAIFYHD
                     EEQKNIALESKEYLENSKIFDSKIVTDIIPATKFYEAEENHQDYYKKRPLNYNMYYKG
                     SGRYNFVKSHWDGNNINREELKKTLTPIQFEVTQNDMTEVPFENEYYDNDKEGIYVDI
                     VSGEVLFSSKDKFDAGCGWPSFSKPVIDKSIVERSDFSQGMYRVEVRSAKGNSHLGHV
                     FDDGPEELGGLRYCINSASLKFIPRENMEALGYGEYLKLFE"
     gene            9210..10340
                     /locus_tag="KRL67_001581"
     CDS             9210..10340
                     /locus_tag="KRL67_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBH1522468.1"
                     /translation="MNLKDFNGKRLKSARIFRAKTIEQLSKETKINKKDLKAFEENKY
                     VPNMENTLKLSNILNFPKEYFYRNENINIVVEDSHFNPQSKLPRVEEISYREKIIIIH
                     KIYSFMENYIKFPKENLPNRKVMSDININDIESLAYKTREFWDLNSTPIVNMVSLLES
                     KGIIISGMNVDRKGATIFTQKQRISKESKYLISLGNDKKSASIRNYTLACELGYIISS
                     ELRIPSKQFSEDEYACAFLLPKESFLEDLIHPEDLDYYVELKKKWIVPISAMIFRAYN
                     LEKINYKKYNYLMNEVDKKGWLIEEPLDKMKGSNPTYLKRAVELLIENKIMSVNSIVS
                     SLEEFGINLYPEDLELLMGLKKGLLSQEANKKSKVIKFDGKK"
     gene            complement(10457..11425)
                     /locus_tag="KRL67_001582"
     CDS             complement(10457..11425)
                     /locus_tag="KRL67_001582"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HBH1522469.1"
                     /translation="MAIKPRKISIVGSGHVGSHCGFSLITQGVCDELFMIDIDESKSK
                     AQALDLADAVSYLPHKVHIEKGTFSDCKDSDIVVISVADSSEGPLRRQNTTRLDLLRP
                     TIGMIKSIVKPIVDSGFDGIFVVISNPVDVVTNYIWEKSGFPKNKVIGTGTALDSTRL
                     RRILSEETGIAQQSIQAYSMGEHGDSQMVPWSHVSIGGKPILNMIKDNPSTYSNLDLP
                     SIVEKNKKTGISIINGKDCTEFGIGTALVEIVKAILHNEKKVLPVSTLLEGQYNERNV
                     FAGVPCVIGKDGIEEIIEINMTEYERNEFNKSCSVLREYIALSKTL"
     gene            11765..12130
                     /locus_tag="KRL67_001583"
     CDS             11765..12130
                     /locus_tag="KRL67_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH1522470.1"
                     /translation="MNYHTKEELMEALRVVSSSIINCEKVQPKFAEGTSQHSLLRNRI
                     KALHISKALIESEDIIDKYTKEELIEALPPITSIISKCEKGQKKFSEETSHHTRFKNI
                     IEAMYISKSLIMDEISKRD"
     gene            12389..13588
                     /locus_tag="KRL67_001584"
     CDS             12389..13588
                     /locus_tag="KRL67_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanyl-tRNA editing protein AlaX-L"
                     /protein_id="HBH1522471.1"
                     /translation="MEKLYYTNQYIRDFTAEIIEIKEVENKYHVLLDKTAFFPGGGGQ
                     FGDLGKIENDDVISVYEDGKNIYHVLDKKPIKIHKVKCSIDWDRREDGMHQHFGQHVL
                     SGCFFKTFNANTTGFHLGNEYSTVDIEGNLEEDKIREIELFANEIIHQNIQVETLLPT
                     KKELKKIWLRRDLPNTDEEIRVIKIGDLDTNACCGVHPKSTGELRMIKIKKWEKHKSS
                     TRIEFLAGKRAVDEVLKRDIYLTKICRYLSCSEEDAINGIVNLNSKVEEALIKKRRLE
                     EVVSTYQVKEMIEKANKVGNILVIKKIYDDEDLKYVNKIANKITEYENSIALLAVKFN
                     DKINLVFACSKNLKDINMGVLLKDAISLIDGKGGGSKVLAQGGGKNNGNLEATLDYAF
                     MKIEKTL"
     gene            13640..14143
                     /locus_tag="KRL67_001585"
     CDS             13640..14143
                     /locus_tag="KRL67_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBH1522472.1"
                     /translation="MEQQKDYIELYENEELIEIREARMDDLDTIVKFNYNLAKETEGK
                     ELDMDVLTKGVKALLLDERKGKYHVYTVFDKVVAQIMYTYEWSDWRNGNFLWIQSVYV
                     DKEYRRKGIFNYLFNYIKNICDKDENIVGMRLYVEKENINAKATYESLNMYECDYNMY
                     EYEVIHS"
     gene            14250..14567
                     /locus_tag="KRL67_001586"
     CDS             14250..14567
                     /locus_tag="KRL67_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH1522473.1"
                     /translation="MGVLKGMFYVFLFLDLVSIFCFFFNKGKIASNKIVFNAIGVLTF
                     VLCFMLFSYYPNNNLIGKFIASLFFIFGVAGVMLKEKNFLYARLLLTVVIVFSTLRLF
                     VIQ"
     gene            14725..15684
                     /locus_tag="KRL67_001587"
     CDS             14725..15684
                     /locus_tag="KRL67_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HBH1522474.1"
                     /translation="MIESKKSEKLLLSIIYIIFLVYILMIIRIILFRDVPIYAIFKGT
                     FRTVNLIPFYTIYQFIVDSNIDFMKATVNIIENIGIFIPMGIFLPIVCKNLNKKTIII
                     TIILVSLAFELTQYIFALGSSDIDDVILNSLGGIIGITIYINMNKLFPNDIKRFKVII
                     ATSLILGVIGLGVISKNYHNLLTFKFRPDRKISKILIEENREIIKDINKDTVDIVGTF
                     ESFKKGIITIKAGSSNKVKRPENLIDSDGNIRIYLNENTKLVSYIITKESKMDIVKYE
                     EFDVKNLDLLRRYDTINVWIDKENQPKDKHGIMASKLLIGLYE"
     gene            15799..16584
                     /locus_tag="KRL67_001588"
     CDS             15799..16584
                     /locus_tag="KRL67_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH1522475.1"
                     /translation="MEIIKYILYFFIYSFLGWTVESIGCSIASKRIINRGFLNGPICP
                     VYGFGAVIVISLLGRFNNVVIVFLLGMILTTVLEYFTGFILETLFHAKWWDYSDRKFN
                     IKGRVCLKNAIYFGIMSVLIIRFIHPFIKDFVGIIPYRILISMSIITTLWTILDLIVT
                     VITLKKLDIKLNLLDDIITDLNDINVKLDKFDRGEIQALLKTINNDELEVREKINKIN
                     SKLDRIESNIILQKRVIKAFPHIKHKKQQEQLEHFKKIINEKR"
     gene            16876..18195
                     /locus_tag="KRL67_001589"
     CDS             16876..18195
                     /locus_tag="KRL67_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HBH1522476.1"
                     /translation="MSEITSQMISTEEKKYVAKTQKIPYYPVAFKSGDGAMLYDYEGN
                     EYVDFLASAGSANVGHGNKEISQAVKEQMDDITQYTLAYFHSDPPVKLAEKLVEIAPG
                     DNDKKVLYSATGSACIDAAIKLARGYTGRTKIISMCESYHGSTYGAISISALSTNMRR
                     KMGPLLPEVYHFHYPDKNRTAKECLDEIEYAFAHYLPAEEVAAIFIEPIAGDAGIIVP
                     PVEWVQGLSKICKENGILLVSDEIQQGMGRTGKWFGIENFGVEADLIVLGKSVGGGLP
                     LGAVVGRTEIMQSLDAPAHLFTLAGNTTVCVAALKSIEIIEKENLLQKSIEMGDYIKA
                     GFEKLKEKYDIIGEIRGIGLSIGVDIVKGKGSNEKHPDATAKICYRCIQTGLIMIFLG
                     QSTLRVQPPLVITKEQVDKAMNIIDSAIDDYLNGRIGDEVYEVTQGW"
     gene            complement(18270..18698)
                     /locus_tag="KRL67_001590"
     CDS             complement(18270..18698)
                     /locus_tag="KRL67_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH1522477.1"
                     /translation="MKDNNNTNKTIQFGEKKASLENNIQKNKNIKLDNTHKVIPYTKN
                     RRKDDKKKGNKKKNTNTSKVNKNNYGHSFFNNKYKYKRKPYVIALYTVILILLAILII
                     TKLTDSTNKGNYSRNTVSDTHKNDIRNIVFNESNNFLICG"
     gene            complement(18789..20165)
                     /gene="hydG"
                     /locus_tag="KRL67_001591"
     CDS             complement(18789..20165)
                     /gene="hydG"
                     /locus_tag="KRL67_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[FeFe] hydrogenase H-cluster radical SAM
                     maturase HydG"
                     /protein_id="HBH1522478.1"
                     /translation="MFINHELINSLLEDAKNSTSDDIEKVLDKADRREKLSYKDIATL
                     LEVEDKKQLDRLFSIAGQIKNEIYGNRVVLFAPLYVSNYCVNECVYCGFSKCNKFKRK
                     KLTMEEIKEEVKILEKMGHKRLALEAGEDPKNCDINYILDCLDAIYSTYNENGNIRRV
                     NVNIAATSVDEYKLLKEKGIGTYILFQETYHKPTFIKMHGQSIKNDYYYHLTAFDRAM
                     EAGVDDVGAGVLFGLSDPKFEVLGLMMHNEHLEEKFGVGFHTISFPRLKKAEGMSLED
                     FPHLVSDDMFKKIVAITRLAVPFTGIIMSTRETAEMRNELLKYGVSQISAGSLTGVGG
                     YKAYEDGDNTEQFEVGDHRSPVEVLKELITDGYIPSYCTACYRKGRTGDRFMSLAKSR
                     QIHNVCTPNALTTLNEFLIDYGDEELKIMGKKLIAEEIGKIEREDIRNIVSNNMTALE
                     RGERDLYL"
     gene            complement(20166..21251)
                     /gene="hydE"
                     /locus_tag="KRL67_001592"
     CDS             complement(20166..21251)
                     /gene="hydE"
                     /locus_tag="KRL67_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[FeFe] hydrogenase H-cluster radical SAM
                     maturase HydE"
                     /protein_id="HBH1522479.1"
                     /translation="MTKNKIKYFIDKLYSINSLSFDELLYLIENIDNEEYNFEQNLRN
                     YLYKQASDMRNKHYGNRVYLRGLIELTNYCKNNCYYCGIQSSNKNIIRYRLTLDEILE
                     CCDIGYKIGYRTFVLQGGEDTYFTDDKICEIVSSIKNKYEDCAITLSLGEKSYDSYKK
                     YFLCGADRYLLRHETATDSHYKKLHPPNIELRTRKECLKNLKEIGYQVGAGFMVESPF
                     QNNEDLVRDLLYLKELNPHMVGIGPFIPHHDTIFKDYKHGDLEKTLLMLSITRLLLPK
                     VLLPATTALASINPSSRNAGLLAGCNVIMPNLSPQEFRKQYSLYDNKAFTGQEACEYH
                     QSLEENIKSLGLEVDYSRGDNIEWRRL"
     gene            complement(21287..22495)
                     /gene="hydF"
                     /locus_tag="KRL67_001593"
     CDS             complement(21287..22495)
                     /gene="hydF"
                     /locus_tag="KRL67_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[FeFe] hydrogenase H-cluster maturation GTPase
                     HydF"
                     /protein_id="HBH1522480.1"
                     /translation="MSLNSTPQSVRVHIGLFGKRNAGKSSIINAITNQSAAIVSDIAG
                     TTTDPVFRPMEILPIGPCVLIDTAGLDDVGELGELRIGKSLDVLEKTDIALLVVDCQI
                     GISQEDLSLIEKFNDKNIPHILILNKIDTIKNQSEILNLTKNKVKCPVVSVSSTDKIG
                     IENLKNEIIRVLPKDSTEFKLVSDLIEPNDLVVLVVPIDKAAPKGRLILPQQQVIRDI
                     LDNGAISIVTKEDSLKETLSNLGKKPKLVITDSQVFPQVDKDTPKDIPLTSFSILFAR
                     QKGDLKELINGAYALENLKDGDKILMAEGCTHHRQTDDIGTVKIPNMIRKKTGKNITF
                     EFSSGVSFTEDINKYALVVHCGACMMNRAGMLSRIEKAKSFNVPIVNYGILIAYIKGI
                     LERSLELFNY"
     gene            complement(22510..22761)
                     /locus_tag="KRL67_001594"
     CDS             complement(22510..22761)
                     /locus_tag="KRL67_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003361768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-only hydrogenase system regulator"
                     /protein_id="HBH1522481.1"
                     /translation="MKKVAVISAILEEPKECQLKFNEVVSGFKGIIKGRMGIPCEEEG
                     VSIVCIAVVGEMDTINSLTGKLGNIPHVSVKTSISKKEI"
     gene            complement(23142..24182)
                     /locus_tag="KRL67_001595"
     CDS             complement(23142..24182)
                     /locus_tag="KRL67_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HBH1522482.1"
                     /translation="MNLNLTNTINLMKKYLDIPSPAGYTENAVLESKKDFKNLGLDTI
                     LTKKGALIATLPGEDDSNQITISAHMDTLGAIVKEISSDGTLKYHKVGGGCWAAIEGE
                     NCTIITRKGKKIRGSVVFKYASTHIYGQTKAATERNEETMIIRLDEKVFTKQDVLDLG
                     ISVGDFVCLDHRFEATESGFIKSRYIDNKSAVAMVLEICRYFKENNLTPKHTTNFYIS
                     NYEEIGHGVSKCLPEKTKEFVAIDIAPTGPSQTSSEHGVTIVAKDNLSLYDFNLRNKL
                     VDVAEDNNLDYAVDVFTFYGSDASEAIRWGEDVQIACVGPGTNNSHHYERTHIEAIEN
                     TLKLLINYMLKE"
BASE COUNT         9190 a         2888 c         3946 g         8268 t
ORIGIN      
        1 taaaaagaat gtataataag tataataatt tgtatttatt attaggaaag gaaaatgtta
       61 atatggatag ttatagacaa cttaaagcta aaataccatg taaggaaaca ggtatagaaa
      121 taaagaggac tttatgcgat atttgttctc caggagtaca ttgtggaatt gatgcatatg
      181 ttaaagatgg taaagtaata aaaatagaag gtacaaaaga acatccaatg aataaagggt
      241 tgttatgtac caaaggtctt tgcaatagag aatatatcta tagagaagat agaataaaaa
      301 cacctctaaa aagaattgga aaaagaggtg aaggaaaatt taaaccaata tcatgggatg
      361 aagcttatag agaaatagca gaaaaattaa atagagtaaa atctgaatat ggagcaaagc
      421 atgtagcttt ttttacgggt tatggaaagt ggtatagacc gtttttacaa aggtttgcat
      481 cttcatttgg ttcacctaat tatggtacag aatctagttc atgttcaaca tcagcacaga
      541 tggctggaaa tgttgctact ggaagaaact ttacaatgca agatatggag aatgcaaaaa
      601 cttatttagg gtggggattt aatccatact actctaaatt tttaaatagt gtgaatctaa
      661 taaaattaaa agaaaaaggt ctaaaattta taattgtaga ccctagaata acaccgacaa
      721 ctcaaaaact agcagattta catttaagaa ttaaacctgg aactgatgga gctttagctc
      781 ttggaatggc gaatctattt atacaaaagg atttaataga tagagaatac atagaaaagc
      841 atgtacatgg atttaatgaa tataaagaat atgtaaaatt atttgattta gatactgtaa
      901 gtagaattac aggagtttct aaagataata tagtaaaagc tgttaagatg ttgtcacaag
      961 gtccattttg tataaatgaa agtgcagctc caatagttca tcatcaaaat ggatttcaaa
     1021 actatagagc aatgatgtct ttatctgcta taacaggtaa ttatgataga ataggtggaa
     1081 acataccaaa acaagattac tttgctggaa agataggtgg attccagaca ctagaaaaag
     1141 agtttgtaaa agaagttcta cctaaaaata taaatgaaag aattggtgga gaaagatttc
     1201 ctctatggaa tgaaatggtg actcaatttc aagctatgga tttacctagg caaatagaag
     1261 aaaagacacc atatgaaata agagctttgt tttgtcatgg gataaatgct agaatgtttc
     1321 ctaattcagg gagacttttt aaagcgttga atagtgtaga tttttttgtg aatatagatt
     1381 tatttatgac agatacatca aaatatgcag atatactttt acctgcgtgt acatcatttg
     1441 agagagaaga atgtaagagt tatccaggag ggtatattac atataccaaa aaagttattg
     1501 ataaattata cgattcaaaa tcagatgtag aaatactttc agatttagca aatgttatga
     1561 atatagatga tgatttatta aaagcaggtt atgaagcaag tataagatat atgtttaaaa
     1621 atacgtgtgt agatgtagat aaacttaaag aaagtgattt accattaaaa tgtaaagatt
     1681 ttaagccgta tatagtaggt tcatatacaa gagaagggta tgatactagt acttctaggt
     1741 ttgaattaaa atctacaatt attgaaaaat ataaagattt tggattagat gcactaccaa
     1801 catatagagg gtttaatgat gatatagata atgaatatac tcatttattg acatcaggag
     1861 caagaatttc aaatgcacta cattcaagac ttcatgacat taaactttca agaatattaa
     1921 aaaatgaagc tagttgtgat atttccattt tagacgcaaa agaattggat gttatcagtg
     1981 gagattatat aaaaataact actaagacag gaagtttgga tgtaaaagtc aatattactg
     2041 atagagtctt atacagaaat atacatatgt atcatggata cagagaagct gatgtaaata
     2101 gtattgtttg tgataatttt gacccttata gtggattccc tgcatataga agttgtaaat
     2161 gcaagattga aaagatgaat agacagatgg agggatataa tgaaaagttt atttaaattt
     2221 aataaagaat tgtgtacagc atgtggagca tgtttgcttg catgtataga ccaaaatgat
     2281 aatgatatag ataatggtga actttttaga tgtgtattta caatcgaaga aggcgaaagt
     2341 gtagaatatt tttcattagc atgtatacat tgtgatgagc caaaatgttt agatatatgt
     2401 cctaaagact gttttaaaaa agaagaagga ttcgttgtat tagacaatca aaactgtata
     2461 gggtgtaaac tttgtgaaaa agcttgtgaa tatggagcct taatttatgg aaatgatggt
     2521 aaggcaaata agtgtaatgg ttgtattgag agatataagt gtggtatgga ttcaccttgt
     2581 gtaatggtat gtcctacagg agcattaaaa tttaataaat aactgtatta aattgctata
     2641 ttacttttaa ataattgtgt taagaattat tttttgctat acttttgtaa tataaaattt
     2701 aaataaaaca aatacaaagt atataaaata tattgttaga ataaagtgtg ctttagatta
     2761 aatttagaaa aataaattag acaggagaac ttaatatggt aaaagtatta gcctttatgg
     2821 gcagtccaag aaaaaaaggg aatgtagaca caatacttga tgagataata aaaggtgtaa
     2881 atgataataa tgttgaagtt aagaaatatt atttaaatga tatgaatatt aaaggatgtc
     2941 aaggttgtct atactgtaga aaagttccta cttgtgcatt taaagacgat atgattgaca
     3001 tatataaaga tataaaaagt gctgaatata taataattgg ttcgccagta tatatatgtc
     3061 aggtatcagc tcaaacaaag ctacttttag atagactgta tcctttaaca gagataaata
     3121 agtctaaaca tacacctagg tttggtcaaa aaaagttaat catggtctat actcaagcag
     3181 cccctgtttc atttttattt aaaaaatatt tcaagtatac ttcaaagcat ttaaagggta
     3241 tgggcttaaa tcactacaaa actatagttg caaccaaagc atttgaaagg aactccacta
     3301 aaaaaaattt caaggtattg agaaaggcct ataaacttgg aatgaaaata gccattgata
     3361 tcaatggata gataaaagtt taatctatag tatagctaaa ggagcattaa ttattaatac
     3421 tccttttttg tataaaggtt tctagaaaat aactaataaa aaacaatatt atttgaatac
     3481 agatattata taagcctaga tatttaactt gtttggaaaa caaaagattt caaacttatt
     3541 tttattaaaa gataacaagc cttcatcccg catcttgcca agctctcttg acatagaact
     3601 tctgtcaaca cataaaaaat cagccatttc atttcttgaa aaagggattg taaacttgtg
     3661 gctacctgtt tgttctatct gctttgaaaa atacataagc aatttctcgc gagttgtttt
     3721 acgagaaagt aattctattt tattgtgaag taaaatattt ttttcagcta ttaattgtag
     3781 catattttca acaagtctat tatgaaatac acatgtagat gaacaaatac cagttacact
     3841 tttaaacttt ataaataaaa cgtcgcaaga agatgttgtg gttactgtaa cattactttt
     3901 atgtagtcta gtacatgcga atacctctcc aaataaatct ccaggcaaga gttcagctac
     3961 aatattccta ttcccctcaa tatcttcctt tattatatga gcacttcctt taagcataat
     4021 tccaatagaa gtaacttcac ttccaacaga gaatattata ctatcttttg ggtaagtctt
     4081 atctacaaca cctaaacatt ccaacatttc ttcaagttca ttacttttaa ttccttcaaa
     4141 tagaggaatt ttttttagta agtctatatg cttttctaaa aattttttca aataatcact
     4201 tccttgttgc atatgcaacc gataatttgt tttcattgta ctatactaag tttataaaag
     4261 caaggggata taaaaatcct actacaagaa atttgaaata aaaaaatata agtattaaaa
     4321 ataaatatat aagaaatcaa tactttaaat taaaaaaatt ttatgaggtg atataaatga
     4381 tgagaaaaat aattaaaatt gatgagggaa agtgtaatgg ttgtggacta tgtgttgaag
     4441 cttgtcatga agaagctata ggaatggtaa atggaaaagc aaaactttta agagatgact
     4501 attgtgatgg gctgggagat tgtctgccaa cttgtccaac aaatgcgatt tcatttgagt
     4561 atagagaagc agcagaatat gatgaagctg cagtaaaagc aaatatggaa gcaaagaaag
     4621 cacaaaagaa aactttagca tgtggatgtc caggttctca gtctaagtca ataaatagag
     4681 gggtttcaaa tagtgcatct ataagcaatg atatagtaga gacaaaaggt tctcaactta
     4741 atcaatggcc agtacaaata aaattagtac caactaatgc atcatattta aagaatgcaa
     4801 gtcttttaat agcagcagac tgtacggcgt atgcatatgg aaattttcat aataaattta
     4861 tgaaaaataa ggttacatta atcggatgcc ctaaacttga tgaagttgac tatgctaaaa
     4921 aacttacaga gattttaaaa gaaaatgata ttaaaaatat agtagtaaca cgtatggaag
     4981 ttccttgttg tggtggtata gtaagtgctg ttaagacagc tctacaaaat agtggaaaaa
     5041 tgataccatg gcaaatagtt actatatctg tagatggaaa aattgtggat ggagaaattt
     5101 aattaagtga attatagaaa aatataataa attttaataa ataaaaaggg aggaataaaa
     5161 aatggaaaaa atgttttgtt ttcaatgtga acaaacagca ggaggtaaag gttgtactat
     5221 aaatggtgtt tgtggtaaaa aggggggtac agccaactta caagacgaac ttacaggagt
     5281 gttgattggt ctttcaagag caactattgg aaacaagaat agaccaacaa atgaaacaga
     5341 taaaattatg atagaggggt tattcactac tataacgaat gtaagttttg atgatgaagc
     5401 tataaaaaga caaattgaaa aaactgagat agaaaaaagt aaacttgtac ctagatgttt
     5461 agactgttca acaacttgta atagaaatga taactatgat atgaaggatt tatggaatga
     5521 taatgaggat atacgttcat taaaatcatt aatcttattt ggattaagag gtatggctgc
     5581 ttatgcatat catgcaatgg tacttggtta tacagacaaa gacgtaaatg agtttttcta
     5641 tgaaggatta atagcagttg gaggagactt gtctattgac gaacttttag gtctagttat
     5701 gaaaactggg gaaataaact taaaatgtat ggaattgctt gatagagcaa atactgaaac
     5761 ttatggaaca ccagaaccga ctcaggtatc tatgaaaatc gagaaaggac catttattgt
     5821 agttacagga catgaccttt atgatttaca caaattatta gaacaaacaa aagataaggg
     5881 tataaatata tatactcatg gtgagttatt accagcacat gcatatccaa aactaaaaga
     5941 atataaacat ctaaaaggta attttggtac agcatggcaa aaccaacaaa aagaatttga
     6001 taatattcct gctccaatat tatttacaac aaactgttta atgcctgtta aagatagtta
     6061 tagagataga gtatttacta cagaagtagt tgcttatcca ggtatggttc atattgatga
     6121 agataaagat ttttctagtg taatagaaaa agctcttgaa cttggaggat ataaagaaga
     6181 tgttcatatg actggtatta atggaggaga tacattaact actggttttg caagaggtac
     6241 agtttcatct gtacaagata aagtagtaga tgctgtaaag agtggagcta taagacactt
     6301 tttattggtt ggaggttgtg atggtgcaaa acctggaaga aattattaca ctgaatttgt
     6361 aaaacaagct cctaaagaca ctgttatatt gacacttgca tgtggaaaat atagatttaa
     6421 tgatttagac cttggagata taggtggctt acctcgttta atggatatgg gacaatgtaa
     6481 tgatgcttat agtgcaatta aagtggcagt ttcattggca aatgcttttg aatgtggagt
     6541 aaatgatttg cctcttagca tggtactgtc atggtatgag cagaaagctg tttgtatact
     6601 tcttacacta cttcatttag gtatcaaaaa tatctatata gggccttctt tgccagcatt
     6661 cttatctaag aatgtattag atatattggt tgataaattt gcattgacac ctatatctac
     6721 tcctgaagaa gatatgaaga agattttagg ataaataaat atatctatac ttaaatatat
     6781 ttatagttaa aatattttta agtatgacac aattaagata aggggttaaa cttaaaattt
     6841 gattgaaaaa atcaattttg agacgaccct ttatttattt tttgtaataa ttgaaaaata
     6901 aacctgcttt cagtatattc tttttaagaa tctttgtatt aaaggtttga atgcaactta
     6961 aatattaaaa gaggaacatt gtaatatgta acttgcataa aatatatata ctatagtgaa
     7021 attattttga ttaaaattta aaatataaga tgttttatgt gggggtatat gtatatgaaa
     7081 aattctaatt ttaatatatt ggcatttgat ggtggtggat taaaaggggc attgagtata
     7141 tccatacttg agaggatagt aaaagagtat ccaaatctat taaacaatat aaatatgttt
     7201 ggaggtacat ctacagggag tctaattgca ctaggacttg catatggtgt aagccctaaa
     7261 gagataaaag aactttactc aattgaaaat tcaaagtata tttttaataa aagttatgca
     7321 gaaatactca gaccaaagta tgaaaacaaa aacttaaaag aagttttatt aagtatattt
     7381 ccagaggaac tagaattaaa agatttaaat aaattagtaa tgataccaag tttttatata
     7441 ggaaatgagg aaaatgcatg gaaaccagtg ttttataaca atatgccaaa ttcttttaca
     7501 aagactagca aagttgtaga tgtagcaatg gcaagtagtg ctgctccagt attttttcca
     7561 acatataatc accatgttga tggaggaata atagcaactg acccaagttt agcgtgtata
     7621 attcatgcca ttgacagtgg atttaagctt aagaatacaa gacttttttc aatagggact
     7681 ggatatgtat ataatagtat aaaggcagat actactgaat ggggagctat tgactggata
     7741 attaataaag aacccgattt accaattata tctattacat tagaaggaaa ttctcaaatg
     7801 agtcagttat tttctcaaaa acttcttgga gataattact atagattaaa tcctaagatg
     7861 gaaaaagatg tagctatgga tgattgtgat gcattagaat atctaatgtc tttaggagaa
     7921 aattgtgata taaaagatgc ttttggatgg ataaagaaaa agagctttta tagaaattag
     7981 attcaatttg aaattgtatt tttacacatg atttcattga gtaggtaaaa ataataaaat
     8041 gcagattatt aagttacttc ttagggaata aataaagtat aatttattta aggagtgatg
     8101 aaaatgataa aacttgctac ttttgcaggt ggatgttttt ggtgcatggt aaaacctttt
     8161 gataagtatg atggagttat taaagtagtt tctggatata caggtggtca tcttgaaaac
     8221 cctacatatc aagaggtatg ttcaggaact acgggtcatt tagaagcaat tcaaataaca
     8281 tatgatgatg atataataag ttatgatgaa cttctaaata tcttctggaa acagattaat
     8341 cctacagatg atggcggaca atttggtgat aggggaactc aatataagac tgctatattt
     8401 tatcatgatg aagagcaaaa aaatatagct ttagagtcaa aagaatatct ggaaaatagt
     8461 aaaatatttg atagtaaaat agtgactgat ataattccag caactaaatt ctacgaagca
     8521 gaagaaaatc atcaggatta ttataaaaaa agaccattaa actacaatat gtattataaa
     8581 gggtctggta gatataattt tgtaaaaagt cattgggatg gaaacaatat aaacagggaa
     8641 gagttaaaaa agactttaac tcctatacaa tttgaagtca ctcaaaatga tatgacagag
     8701 gtgccttttg aaaatgaata ttatgataat gacaaagaag gaatttatgt tgatattgtg
     8761 agtggagaag ttttattttc ttcaaaagat aaatttgatg ctggatgtgg gtggccaagt
     8821 ttctcaaagc cagttataga caaatctatt gttgagagaa gtgatttctc acaaggtatg
     8881 tatagagttg aggtaagaag tgctaaagga aattctcatt taggtcatgt atttgatgat
     8941 ggtccagaag aacttggggg tttaaggtat tgtataaatt ctgcttctct taaatttata
     9001 ccaagagaaa atatggaagc tttaggatat ggagaatatt taaaactttt tgagtaatta
     9061 ggaatattta ttataaactt agctaaaagg tgaaaatata agaaaatatt ttcacctttt
     9121 gtttttttgt caataatatg ataaattatt aattgtaatc ttatttatac caatagatta
     9181 agaatttaat tttgaaagag agaaaacaaa tgaatttaaa agactttaat ggaaagagat
     9241 taaaatcagc tagaattttt agagcaaaga cgatagaaca gttgtctaaa gaaactaaaa
     9301 taaataaaaa ggatttaaaa gcttttgagg aaaataagta tgtaccaaat atggaaaaca
     9361 cattaaaatt atctaatata ttaaattttc caaaagagta tttttataga aatgaaaata
     9421 taaatatcgt tgtagaggat agccacttta accctcaatc taaactacct agagtagaag
     9481 aaatttctta tagagaaaaa ataattataa tacataaaat atattcgttt atggagaatt
     9541 atattaagtt tccaaaagaa aacttgccaa atagaaaagt aatgtcagat attaatataa
     9601 atgatataga atctcttgca tataagacta gagagttttg ggatttaaat agtaccccaa
     9661 tagtaaatat ggtaagtttg ttagagtcta aagggattat aatatcaggt atgaatgtag
     9721 atagaaaagg ggcaactatc tttactcaaa aacagagaat tagtaaagaa tctaaatatc
     9781 taatatctct gggtaatgac aaaaagtcag catcaattag aaattatact ttggcatgtg
     9841 agcttggata tataatatca agtgaattaa gaataccaag taagcaattt tcagaagatg
     9901 agtatgcttg tgcattttta ttgccaaaag agagtttttt agaagatttg attcatccag
     9961 aagatttgga ttattacgta gaactaaaga aaaaatggat tgtacctata tctgctatga
    10021 tttttagagc atataatcta gaaaagataa attacaaaaa atacaattat cttatgaatg
    10081 aagtggataa aaaaggatgg ttgatagaag aaccactaga caaaatgaaa ggttcaaatc
    10141 caacgtattt aaaaagagca gttgaattat tgatagaaaa taaaataatg agtgtaaata
    10201 gtatagtatc aagtttagag gaatttggaa taaatcttta tccagaagat ttagaattat
    10261 tgatgggatt gaaaaaagga ttattatctc aagaggcaaa taaaaaaagt aaagttataa
    10321 aatttgatgg taaaaaatag gttgctattt tagtattaaa attaaaagca ggtataaatg
    10381 atttaaacat taacttagtt aaaagtcttt tcaataagtc atttacctgc ttttagtgta
    10441 agaaagaatc ttgaatctaa agtgtttttg aaagtgcaat atattctctt aaaacagaac
    10501 aagatttatt aaattcgttt cgctcatatt cagtcatatt gatttcaata atttcttcaa
    10561 taccatcttt tcctataaca catggaacac ctgcaaatac atttctttcg ttatattgac
    10621 cttctagtaa tgtagaaact ggaagaactt ttttctcatt gtgtagtata gcttttacaa
    10681 tttctactaa agcagtacct ataccaaatt ctgtacaatc tttaccattt attatactta
    10741 tccctgtttt tttatttttt tcaactattg atggtaagtc taaattgcta taagtacttg
    10801 ggttgtcttt tatcatgttc aagattggtt taccaccaat tgaaacatga gaccaaggaa
    10861 ccatttgaga atctccatgt tcacccatag aataagcttg aattgattgt tgagcgattc
    10921 cagtttcttc agataaaatc cttctaagtc ttgttgaatc taaagctgtg ccagttccaa
    10981 tgactttgtt tttaggaaag ccagattttt cccaaatata atttgtaact acatcaacag
    11041 gattagatat gactacaaat ataccatcaa agccagagtc aactatgggt tttactatag
    11101 attttatcat tccaatagtt ggtcttagca aatctaatct agttgtgttt tgtctacgta
    11161 aaggcccttc tgaagagtct gcaacactta tcactactat atcagaatct ttacagtcag
    11221 aaaaagttcc tttttctata tgtactttgt gtggcagata tgaaactgca tcagctaaat
    11281 ctaaagcttg agcttttgat ttagattcat ctatatcaat cataaagagt tcatcacaaa
    11341 ctccttgagt tatcaaactg aatccacaat gagaaccaac atgccctgaa cctacaatag
    11401 atatttttct tggctttatt gccattttaa atcactcccc ctaaaataac gatacattta
    11461 taagaaaatt atagatgtta ttaaaagctg tttttatata ttttaatatt ctattatttc
    11521 agaaaattta tttaatatga ttttatacta aaatttttaa aaaagcaatg ttaatatact
    11581 ctctattaat gaatgttaaa gtattatatg tatattaaca attattaaaa tatgtaatgt
    11641 tactgatagt tagaatattt aagtgttaaa agatgtgtat aaataataat gatgtcagtt
    11701 gataatgata ttaaattgag gtatactgtt tctctaaatt aaaaaataaa ggagtaaatg
    11761 tgttatgaat tatcatacaa aagaagaatt gatggaagca ctgagagtag tatcttcaag
    11821 tattattaat tgtgaaaaag tacaaccaaa atttgcagag gggacttccc agcattcact
    11881 tcttagaaat aggataaaag cattgcatat ttctaaggca ttgatagaat ctgaagatat
    11941 tatagataaa tatacgaaag aagaattaat agaagcttta ccccctataa cttcgattat
    12001 tagcaaatgt gaaaaaggac agaagaaatt ttctgaggag acatctcatc atacacgttt
    12061 taagaatatt atagaagcaa tgtatatttc aaaatcatta ataatggatg aaattagtaa
    12121 aagagattaa agcttatttt gcaatttaac atgttttaat atacatttta tatataagaa
    12181 aataagtaag catacatgtt ataaaatatt ggaacaatat gcaattataa atttgttaac
    12241 actaatatct atatgttaaa atatagtaga agatgttagt aaataatagg attagttcta
    12301 ttttaataca attaaattaa gcttggcttg aaatttgtat ttaaaaagta aagtagtcta
    12361 taattaagaa aatggagata gaattataat ggaaaagtta tattatacaa accagtatat
    12421 aagagatttt acagcagaaa taattgaaat aaaagaagta gaaaataagt atcatgtttt
    12481 attagataag acagcttttt ttcctggtgg aggaggtcag tttggagacc tggggaaaat
    12541 agagaatgat gatgtaataa gtgtgtatga agatggaaaa aatatatacc atgtattaga
    12601 taaaaaacct ataaagatac ataaggtaaa gtgtagtatt gattgggata gaagagaaga
    12661 tggaatgcat caacattttg gacaacatgt gttatctgga tgttttttta agactttcaa
    12721 tgcaaatacg acaggttttc atttgggaaa tgagtatagt acagtagata tagaaggtaa
    12781 tttagaggag gataaaatta gagagataga gttgtttgcc aatgaaatta ttcatcaaaa
    12841 tatacaagta gagacattac ttccaacgaa aaaagaacta aaaaagattt ggttaagacg
    12901 tgatttgcca aatactgatg aagagataag agtaataaaa ataggtgatt tagatacgaa
    12961 tgcatgttgt ggagtgcatc caaaatcaac tggtgaattg agaatgataa aaataaagaa
    13021 atgggaaaaa cataagtcaa gtacaagaat agagtttcta gcaggtaaaa gggcagtgga
    13081 tgaagtattg aagagagata tatatttgac taaaatatgt agatatttaa gctgtagtga
    13141 ggaagatgct ataaatggta ttgtaaatct aaatagtaaa gtggaagagg ctttaattaa
    13201 aaaaagaaga cttgaagaag tagtatctac atatcaggtc aaagaaatga ttgaaaaagc
    13261 aaacaaggtt ggcaatattt tggtcattaa aaaaatttat gatgatgaag atttgaaata
    13321 tgtaaataag atagcaaata aaattacaga atatgaaaat agtattgcat tattagctgt
    13381 taaatttaat gacaaaataa atttagtttt tgcatgttct aaaaatttaa aagatattaa
    13441 tatgggagtt ttattaaagg atgcaataag ccttatagat ggcaaaggtg gtggaagtaa
    13501 agtattagct caaggaggag gtaagaataa tggaaattta gaagctacac ttgactatgc
    13561 atttatgaag atagaaaaaa ctctataaat atattatata aaaaacctta atagataaga
    13621 tagatatgga ggaataagta tggagcaaca aaaggactac atagagttat atgaaaatga
    13681 agaattaata gagataagag aagcacgtat ggatgatttg gatacaatag taaaatttaa
    13741 ttataatttg gctaaagaaa ctgaaggaaa agaacttgat atggatgttt taactaaagg
    13801 tgtaaaagca ttgttattag atgagagaaa aggaaaatat catgtatata cagtatttga
    13861 taaagttgtt gcacagatta tgtatacata tgagtggagt gattggagaa atggaaattt
    13921 cctttggatt cagagtgtat atgtagataa ggaatataga agaaaaggta tctttaatta
    13981 tttatttaat tatataaaaa atatatgtga taaagatgaa aatattgttg gaatgagatt
    14041 atatgtagaa aaagaaaaca taaatgcaaa agcaacatat gagtctttaa atatgtatga
    14101 gtgtgattat aatatgtatg aatacgaagt aatacattcg taatataaat ttgtaataca
    14161 gttgtactaa tgttataaag cattgattat attgacgatt atatgaaata aactggatat
    14221 tataattaga atttttaggg ggtataaaaa tgggcgtttt aaaaggaatg ttttatgtat
    14281 ttttgttttt agatttagtg tcaatattct gtttcttttt taataaagga aaaatagcat
    14341 ctaataaaat tgtgtttaat gcaataggag tacttacgtt tgtactttgt tttatgcttt
    14401 ttagttatta tcctaataat aatcttattg gaaaatttat tgcttctcta ttttttatat
    14461 ttggagttgc aggagttatg ttgaaagaaa aaaatttttt atatgctaga cttcttttaa
    14521 ctgtagttat agttttttct acactaagat tgttcgttat tcaataacat gtattcgtat
    14581 gaaaatttgt taaagtctac acatttaagt gtaggctttt ttgctttttt atttaaaaat
    14641 taaggaaatt ttaaggaaat tttaaaatcg tcttaaatat ttattgatat atttaaaata
    14701 ttaatagcga aaggagactt atcagtgata gaaagtaaaa agtcagaaaa attgttactg
    14761 tcaattatat acataatatt tttggtatat attttaatga ttataagaat aattttattt
    14821 agagatgtac caatttacgc tattttcaag ggaactttta ggactgtaaa tttaattcca
    14881 ttttatacaa tatatcaatt tatagtagat agtaatattg actttatgaa agctactgta
    14941 aatattatag aaaatatagg tatatttatt cctatgggaa tttttttacc tatagtatgt
    15001 aaaaatctaa ataaaaaaac tataattata acaattatac ttgttagctt agcatttgaa
    15061 ttaacacaat atatttttgc tcttggaagt agtgatatag atgatgttat tttaaattca
    15121 ttaggtggca taattggaat tactatttac ataaacatga ataaattatt tcctaatgat
    15181 attaaaagat ttaaggttat aatagctact agtttaattt taggagttat aggattagga
    15241 gttatttcta aaaattatca taacttacta acttttaaat ttagacctga tagaaaaata
    15301 tcaaaaatat taattgaaga aaatagggaa ataattaagg atataaataa agatacagta
    15361 gacatagttg ggacatttga gagctttaaa aaaggaataa taactattaa agcaggctct
    15421 agtaacaaag taaagagacc agagaattta attgattctg atggaaatat aagaatttat
    15481 ttaaatgaaa atacaaaatt agtatcttat ataattacca aagaatcaaa aatggatata
    15541 gttaaatatg aagaatttga tgtgaagaat ttagatttat taaggagata tgatactatt
    15601 aatgtttgga ttgataaaga aaatcagcca aaggataaac atggtattat ggctagtaaa
    15661 ttattaattg gattgtatga ataaaatatt aaataagaat aaaagaataa caaatataaa
    15721 aaacgaaaaa actatttcta taattatttt tacgttgtgt ataaaataag gacactggtg
    15781 aaggagtaga gataaattat ggagataata aaatatatct tgtacttttt tatatattca
    15841 tttttaggat ggacagtaga aagtatagga tgttctatag caagcaaaag aataatcaac
    15901 agaggttttt taaatggtcc aatatgccct gtttatggtt ttggagcggt aattgtgata
    15961 tcactattgg gaagatttaa taatgtagta atagtatttc tattaggaat gattttaact
    16021 acagtactag aatattttac aggatttatt ttagaaacct tatttcatgc taaatggtgg
    16081 gattattctg acagaaaatt caatataaag ggtagagtat gtttaaaaaa tgcaatatac
    16141 tttggtataa tgtctgtttt aataataaga tttatacatc catttataaa ggattttgta
    16201 ggtataattc catatagaat attaatttca atgtctataa ttacaacact ttggactata
    16261 cttgatttga ttgttactgt tataacactt aaaaaattag atataaagtt aaacttacta
    16321 gacgatataa ttacagacct taatgatata aacgtgaaat tggataagtt tgatagagga
    16381 gaaatacaag ctttacttaa aacaataaat aacgatgaat tagaagttag agaaaaaata
    16441 aacaaaataa atagtaagtt ggaccgtata gagtctaata ttattttaca aaaaagagtt
    16501 ataaaagctt ttccacatat aaaacataaa aaacaacaag aacaactaga acattttaaa
    16561 aaaattatta atgagaaaag gtaattatac aatttaatga aaaagaatag aataatggta
    16621 tttataagat gattttcaga ggactctata aaaatagaat agagtccttt attttttgaa
    16681 aaataatgtt tatttgataa aaataaaata tattgtttct gaataacaaa tattttgaaa
    16741 attataaaaa catatattga caatttgtgt caaaaatact aacattaagt atataaagaa
    16801 ttttatatga gaatatcttt ataaatcgca ataattttaa aacataaatt tatttaaaga
    16861 ggggatgatt tgccaatgag cgagataact agtcaaatga taagtacaga agaaaaaaaa
    16921 tatgttgcta aaactcaaaa gataccttat tatccagtag catttaaatc aggtgatggt
    16981 gcaatgctat atgattatga aggtaatgaa tatgtagatt ttttagcaag tgcaggctct
    17041 gcaaatgtag gtcatggtaa taaggaaatc tcacaagcag ttaaagaaca aatggatgat
    17101 ataacacaat atacacttgc atatttccat agtgatcctc ctgtaaaatt agctgagaag
    17161 cttgtggaaa tagcccctgg agataatgat aaaaaagtac tatacagtgc aactggttct
    17221 gcttgtatag atgctgctat aaaattagca agagggtata ctggaagaac aaagataata
    17281 tctatgtgtg aatcttatca tggaagtact tatggagcta tatctatatc tgctttaagt
    17341 acaaatatga gaagaaaaat gggtccattg ttaccagaag tttatcactt ccattatcct
    17401 gataaaaata gaacagctaa agaatgttta gatgagatag aatatgcttt tgcacactat
    17461 ctaccagcag aagaggtagc tgcaatattt atagagccaa ttgcaggaga tgcaggaata
    17521 atagtgcctc cagtagagtg ggtacaaggt ttaagtaaaa tatgtaaaga aaatggaata
    17581 ttacttgtaa gtgacgaaat acaacaaggt atgggaagaa caggtaagtg gtttggtata
    17641 gaaaattttg gtgttgaagc agaccttata gttcttggaa aatctgttgg aggtggatta
    17701 cctctaggag cagttgttgg aagaacagaa ataatgcaaa gtcttgatgc accagcacat
    17761 ttatttactt tagctggaaa tacaacagtg tgtgtggctg ctttaaaatc tatagagata
    17821 attgaaaaag agaacttact tcaaaagagt atagaaatgg gagattatat taaagcagga
    17881 tttgaaaaac taaaagaaaa atacgatata attggagaaa taagaggaat aggattatct
    17941 ataggagttg atatagttaa aggaaaaggt tctaatgaaa aacatccaga tgctacagct
    18001 aagatatgtt atagatgtat acaaactgga ttaataatga tattcttagg acaatctact
    18061 ttaagagtgc aaccacctct agtaataact aaggagcaag tagataaagc aatgaacatc
    18121 atagattctg caatagatga ttatttaaat ggtagaattg gtgatgaagt atatgaagta
    18181 actcaaggtt ggtaaaataa atattaaatg aaaataggag ttatctcaat tttagaaagt
    18241 aatttctgaa atgagataac tcttttaagt tacccacaaa taagaaaatt gtttgattca
    18301 ttaaagacta tatttcggat gtcgtttttg tgagtatcag agacagtatt acgagaataa
    18361 ttacctttat tcgtactatc tgttaattta gttataatta agatagctaa aagaataaga
    18421 attactgtgt ataaagcaat aacatatggt tttcttttat atttgtattt gttattgaaa
    18481 aaagaatggc cataattatt tttatttact ttagatgtat ttgtattctt tttcttgtta
    18541 cctttctttt tatcatcctt tcttctattt ttagtgtaag gaatgacttt gtgtgtatta
    18601 tcaagtttga tatttttatt tttttgaata ttgttttcca ggctagcttt tttttctcca
    18661 aattgaatag ttttattggt attgttgtta tctttcaatg ttaatctccc tcccttaaac
    18721 tttcccttta gataatatta ctactaacct atggaatata caataaataa tatataagta
    18781 ataaaatttt ataggtataa atctctttct ccacgctcta aggcagtcat attgttagat
    18841 actatgtttc ttatatcttc tctttctatc ttacctattt cttctgcgat aagtttttta
    18901 cccataattt taagttcttc atctccataa tcgattaaaa attcatttaa tgtagtaaga
    18961 gcatttggtg tacatacatt atggatttgt cgacttttag ctaagctcat aaatctatca
    19021 ccagttctac ccttgcgata acaagctgta caataactag gtatatatcc atcagtaatc
    19081 agttctttaa gcacctcaac agggcttcta tggtctccaa cttcgaattg ttctgtattg
    19141 tcaccatctt catatgcttt atatcctcca acacctgtta gagaacctgc actaatttgt
    19201 gatacaccat attttagtaa ctcatttctc atttcggcag tttccctagt ggacattatg
    19261 attccagtaa aaggaacagc caatctagtt atagctacta tttttttgaa catatcatca
    19321 gaaactaagt gaggaaaatc ttctaagctc ataccttcag cttttttcaa tctagggaaa
    19381 gatatagtat ggaaacctac accaaatttt tcctctaagt gttcgttgtg catcataagt
    19441 cctagaactt caaatttagg gtcagataaa ccaaataaaa ctcctgcgcc aacatcatca
    19501 acgccagctt ccatagctct atcaaaagct gttaaatggt aataatagtc attttttata
    19561 gattgaccat gcatttttat aaatgttggt ttgtgataag tttcttggaa taatatataa
    19621 gttcctattc ctttttcttt tagtaattta tactcatcta cacttgtagc tgctatgtta
    19681 acgtttaccc ttcttatatt tccgttttca ttatatgttg aataaattgc atctaagcaa
    19741 tctaagatgt aatttatatc acaattttta gggtcttcac cagcttctag agcaagtctt
    19801 ttatgaccca ttttttctaa tattttaacc tcttccttta tctcttccat ggtaagtttt
    19861 tttcttttaa atttgttgca tttactaaaa ccacaataaa cacattcatt tacacagtag
    19921 tttgatacat aaagaggggc aaataaaacc actctattac catatatttc attttttatt
    19981 tgcccagcta tactaaataa tctatctagt tgttttttat cttctacttc aagaagtgta
    20041 gctatatctt tataactaag tttttccctt ctatcagcct tatcaagtac cttttcaata
    20101 tcatcagaag ttgaattttt tgcatcttct agtaaagaat taataagttc atggtttatg
    20161 aacatttaca aacgcctcca ttcaatatta tcaccacgag aatagtcaac ttcaagtcct
    20221 aatgacttga tattttcttc taaactttgg tgatattcac aagcttcttg accagtaaag
    20281 gctttgttat cgtaaagaga atattgtttt ctaaattctt gaggagaaag gtttggcata
    20341 attacattgc aaccagctaa aagacctgca tttctactac tagggtttat agatgccaag
    20401 gctgttgtag cgggtaataa aacttttgga agtagaagtc ttgtaattga aagcataagt
    20461 aatgtctttt ctaaatctcc atgtttataa tccttaaaaa ttgtatcatg gtgtggaata
    20521 aaaggtccta ttccaaccat atgaggattt aactctttta ggtataataa atcacgaaca
    20581 aggtcttcgt tgttctgaaa tggtgattca accataaatc cagcacctac ctgataacca
    20641 atttctttaa ggttttttag gcattctttt ctagttctaa gctcaatgtt tggcggatgt
    20701 aattttttat aatgactgtc tgtagcagtt tcatgtctta aaagatatct atctgcacca
    20761 cataaaaagt attttttata cgaatcataa ctcttttctc ctagtgatag agttatagca
    20821 caatcctcat atttattttt tatacttgaa acaatttcac atattttatc atcagtaaaa
    20881 tatgtatctt caccaccttg caaaacgaat gttctgtatc ctattttata acctatatca
    20941 caacattcaa gtatttcatc aagtgtaagt ctgtatctta tgatattttt attagaagat
    21001 tgtattccac aatagtagca attattttta caatagtttg taagctctat aagtcctctc
    21061 aagtagactc tatttccata atgtttattt ctcatgtcgc tagcttgttt ataaagatag
    21121 tttcttaaat tttgttcgaa gttatattcc tcattgtcga tattttctat taaatatagt
    21181 agttcatcaa aacttaatga attaatgctg taaagtttat ctataaagta tttaatctta
    21241 tttttagtca tattcaaata tgtaattatt aagtatagtt aaatttttaa taattgaaaa
    21301 gttcaagtga tctttccaaa atacctttta tatatgctat taagatacca tagtttacaa
    21361 taggtacatt gaaagatttt gccttttcta ttctagataa cattccagct ctattcatca
    21421 tacaagcgcc acagtgaact acaagggcat atttatttat atcttcagtg aaggatactc
    21481 cagaactaaa ttcaaaagta atatttttgc cagttttctt tctaatcata ttaggaattt
    21541 ttactgttcc aatatcatca gtttgtctat gatgggtaca tccttcggcc atcaagattt
    21601 tatcaccatc ttttaaattt tctaaagcat aagctccatt aatcaattct ttaaggtctc
    21661 ccttttgtct agcaaacaat atagaaaaag atgtaagggg aatatctttt ggtgtatctt
    21721 tatctacttg tggaaatact tgagagtcag taataacaag ttttggtttt ttgcctaaat
    21781 tagatagagt ttcttttaaa ctatcttctt ttgtaactat agaaattgcg ccattatcca
    21841 aaatatctct tataacttgc tgttgaggaa gtataagtct accttttggt gctgctttat
    21901 ctattggaac aactaaaaca actaaatcat ttggttcaat taaatctgat accaatttga
    21961 actcagtgct atctttaggc aaaactctta ttatttcatt ttttaaattt tcaattccaa
    22021 ttttatctgt tgatgagaca gaaacaactg gacatttaac tttattttta gttaaattta
    22081 aaatttctga ttgattctta atagtatcaa ttttgttaag aattaaaata tgagggatat
    22141 tcttatcatt aaacttttca attagtgata aatcttcttg tgatatacca atctggcaat
    22201 ccactactag taaagctata tctgtttttt ctaaaacatc tagtgattta cctattctaa
    22261 gttcaccaag ttcaccaaca tcatcaagac cagcagtatc tataagaaca caaggtccta
    22321 taggaaggat ttccattggt ctaaatactg ggtctgtagt agttccagca atatctgaga
    22381 ctattgcagc actttgattc gttatagcat ttattatgct agatttacct gcatttcttt
    22441 taccaaagag acctatatgt actcttactg attgtggagt agaatttaga ctcatttgta
    22501 ctcaccacct taaatttctt tttttgatat actagttttt acagatacat gaggtatgtt
    22561 gcccaattta ccagtcaaac tattaatagt atccatttcg ccaacaactg ctatacatac
    22621 gattgatacg ccttcttctt cgcaaggaat gcccattctt cctttaataa tacctttaaa
    22681 acctgataca acttcattga atttaagttg acattctttt ggctcttcca aaatagcact
    22741 tataactgca acctttttca taaatatcct cctaactcag aaaatatgtc ttaaacaaat
    22801 ccaaaatcaa aaaacttttc tttgtaaaga aaagtttcta caaaagaaaa gttattaatt
    22861 acacaagtat aatgtgctat attaaactat ttccttacag attagataac tcttttctgg
    22921 tagggttagc ttgtaaaaat ttaatttaac aacaattaac tgaagaaaat ttttatacaa
    22981 tttatataaa ttataataaa aattaactaa agaaaattga agttacttat ataatagtag
    23041 taaattgaaa ttaagtctat agaaaaacca atttttgtat aaaaataaaa attaaagata
    23101 gttatttgtt aaaaattgac tttgataaaa tatataatta attattcttt aagcatataa
    23161 tttataagta attttagtgt attttcaata gcttctatat gtgttctttc ataatggtga
    23221 ctattgtttg ttccaggacc aacacatgct atttgaacat cttctcccca acgaatagct
    23281 tctgaagcat ctgaaccata gaaagtaaat acatcgacag cgtagtctaa attattgtct
    23341 tctgcaacat cgacaagctt gtttcttaaa ttaaaatcat ataatgataa attatctttt
    23401 gcaactatag ttacaccatg ttcactagat gtttgacttg gcccagtagg cgctatatct
    23461 attgctacaa attcttttgt tttttcaggt aaacatttag aaacgccatg tccaatttct
    23521 tcataattac ttatataaaa gttagtagta tgcttaggtg ttaaattgtt ttctttaaaa
    23581 tatctacata tttcaagaac cattgctaca gctgatttat tatctatata tctagatttt
    23641 ataaaaccag attctgtagc ctcaaatcta tggtctaggc atacaaaatc cccaacactt
    23701 attcctaaat ctaatacatc ttgctttgta aatacttttt catcaagtct aataatcatt
    23761 gtttcttcat ttctttcagt ggcagctttt gtttgaccat atatatgagt agaagcatac
    23821 ttaaatacaa cacttcctct gattttttta ccttttctag ttataatagt acagttttct
    23881 ccctctatag ctgcccaaca tccaccacca actttatggt atttaagagt accatcagat
    23941 gatatttctt ttactatagc tccaagtgta tccatatgtg cagatattgt tatttgattt
    24001 gaatcatctt caccaggaag agtagctata agtgcacctt tttttgtaag aatagtgtca
    24061 agacctaaat ttttaaaatc ttttttgctt tctaaaactg cattttcagt atatccagct
    24121 gggcttggaa tgtccaaata ttttttcatt aaatttatag tattagttaa atttaaattc
    24181 atattgtttc ctcctaaata aatatattta tacaaattat aacataaata caaatgttct
    24241 gaaaactaaa attaaaagaa tgaaatctaa acatatttaa aaaaaaagta ta
//
LOCUS       DAEPXF010000046        24227 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain HB27
            SAMN13376389-rid12479423.denovo.048, whole genome shotgun sequence.
ACCESSION   DAEPXF010000046 DAEPXF010000000
VERSION     DAEPXF010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13376389
            Sequence Read Archive: SRR10518432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 24227)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24227)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001069624.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001069624.1
            Genome Coverage       :: 315x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/15/2021 01:54:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,165
            CDSs (total)                      :: 4,076
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 89
            rRNAs                             :: 10, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 10 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 19 of 69
            Pseudo Genes (incomplete)         :: 44 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 10 of 69
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24227
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13376389-rid12479423.denovo.048"
                     /strain="HB27"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="China: Hebei"
                     /collection_date="2017"
     gene            complement(159..2546)
                     /gene="cadA"
                     /locus_tag="KRL67_001596"
     CDS             complement(159..2546)
                     /gene="cadA"
                     /locus_tag="KRL67_001596"
                     /EC_number="7.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadmium-translocating P-type ATPase"
                     /protein_id="HBH1522483.1"
                     /translation="MEANNLIKKEFILGGLNCAHCAEEINNKVSKLQEVKSSNLNFIN
                     KKLTVNIKESFNEDTTIEKIIDIIDSTEPGLDIQISSKENASSKISIKKELILGGLNC
                     AHCAEEINNKVSKLKEVESSNLNFVNKKLTVNISNNFEADDVINKIKEIINSTEPGLD
                     IQVESTNKVKGRTTEKPGAVNDTNKKELIPLIIGALVYIFGIYQTATGYESQFSNIVF
                     IVAYVIVGGDVLLRAIRNISKGRVFDENFLMALATVGALAIGELSEAVGVMLFYKVGE
                     YLQGVAVGKSRKSITSLMQIRPDYANLKVNSEVKVVSPEEVNVGDIIVVKPGEKVPLD
                     GVVVDGISMLDTSALTGESVLREVEKGDEILSGVINKNALLSIEVTKSFGESTVSKIL
                     DLVENSSIKKSKTENFISKFSRYYTPIVVIAALLIAFVPPLVISGEVFSDWLYRGLIF
                     LVVSCPCALVLSIPLSFFSGIGFASKNGILIKGSNYLEALRSVDTVVFDKTGTLTKGV
                     FNVTKLNPEGISEEELLEYAAIAEVNSNHPIAKSILSYYNKKIDLDTIDSYEEIAAYG
                     IRVKHNGNFILAGNEKLMKKENISYSSAKEVGTVVYIAVDKVYRGYIVISDEVKEDSK
                     NAIRSLKAIGVKEVVMLTGDNEKVAKNIAQELELDTVYSNLLPNEKVDRLEDLYEGRT
                     EKEKIAFVGDGINDAPVLARADVGIAMGGLGSDAAIEAADVVLMTDEPSKISKAIEIA
                     NKTNKIVWQNIIFALGVKIIVMILGAGGVATMWEAIFADVGVALIAVVNAMRAMR"
     gene            complement(2569..2934)
                     /locus_tag="KRL67_001597"
     CDS             complement(2569..2934)
                     /locus_tag="KRL67_001597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBH1522484.1"
                     /translation="MREEINDCNCNIVHEEIVTEAKSTMPDEEMLYDLAELFKVFGDT
                     TRVKILYALFANEMCVCDIASLLNMTHSAISHQLRVLKQARLVKFRREGKTVYYSLDD
                     SHISQIFDCGLNHIRETYK"
     gene            3134..3985
                     /locus_tag="KRL67_001598"
     CDS             3134..3985
                     /locus_tag="KRL67_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH1522485.1"
                     /translation="MSNKKKKDLDTSYMPYLDPANYADYITNEEIPQDELRNDPQISN
                     GYTKIPNNQNINNLYPNNVNHYPKSSNSPNNLPNTNNTSGNMNYNTSATNNMPNNMNN
                     NTGMPNNMSNNMNSNMGMPNNMNSNMGMPNNMNSNMGMPNNMPNNMNSNMGMPNNMNS
                     NMGIPNNMSNNMNSNMGMPNNMSGNMNNMYGNTSMPNNMNSNMGYNMNNTMGIPNFSC
                     NQNMPPNVLMMIPGVICHNTMQGMPVVMPSTMPPNIYPTPYGSSNMSIQGIPQTTNIE
                     EFDEEEM"
     gene            complement(4422..5510)
                     /locus_tag="KRL67_001599"
     CDS             complement(4422..5510)
                     /locus_tag="KRL67_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH1522486.1"
                     /translation="MNFNFAKATNSRLMGSLGLIINWIDDENNHFCQYFLLDAEGLGL
                     ADYVSLNNPTQEEAYMEEERLMGGFGSDRVELTKDESLFLVSYFGNKNLYYDKLLPGD
                     KCEYIDIIKNYKTDLTIEKLYNKICKRVDEEVEFINYMTMRFIAWDRESLKYFSGSDE
                     IANMHITNINGTLLKNVVSDKGQGRYISEALYEDNDGYYICKIAFCISKCYETGFRIN
                     SLLVTDKEPMYDFEVFDEISKSEFVSVYSVNSPEEFAGVFYRENPFLLKSNMNEGIFF
                     TRFNFNNDHVKENVYIINNDMKAIYYLMGNKFFIGTYNENDCNYINEISQSNYSDYIK
                     FEEKFFFEQNALYDFAESGSLDFDDFLE"
     gene            5635..6363
                     /locus_tag="KRL67_001600"
     CDS             5635..6363
                     /locus_tag="KRL67_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IB family hydrolase"
                     /protein_id="HBH1522487.1"
                     /translation="MKNIAAFFDIDGTLYRDSLMVEHFKKLIKYDIIDQKNWYKHARD
                     TFMDWDKRQGNYDDYLLEICDLYVDSLKGLDKTCINFTSDQVIKLKADRVYKYTRSRI
                     EWHLNQGHIVIFISGSPGFLVEKMAKKYNVTDFLGSDYVFENNIFTGTVIPMWDSISK
                     NNAINDFVVKYDLDLSNSYAYGDTNGDINMLKRVGNPIAINPTKELLSQISLDEEISK
                     NVEIIVERKDIIYSLSTDVNIVDI"
     gene            complement(6565..7056)
                     /locus_tag="KRL67_001601"
     CDS             complement(6565..7056)
                     /locus_tag="KRL67_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH1522488.1"
                     /translation="MSIKVAYGGVLLALNVILLTLTNIIPVNTLFIMGLASLLVSIVI
                     MEWGFKSGIAFYIGSIVLGFIVMASKSQWIVYSLTFGIYGIVKYLIEKDKSIYIEYFM
                     KLVFANIMILILYFLLRTIVYIPINIFIIGSFEIAFIVYDYVYSSFIGYYNNRLRKML
                     FKK"
     gene            complement(7067..7510)
                     /locus_tag="KRL67_001602"
     CDS             complement(7067..7510)
                     /locus_tag="KRL67_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH1522489.1"
                     /translation="MYSNTYNNENFMRARAFIENSEFKKAYDFLKTLTDKCAEWYYLT
                     GFSAMNIGYYEEGEDFLKRAKFMEPENSEYSDALRSYTQYRNDYSNRADNYNRRRRND
                     LDGCCCCCCDDCCCCLGDDCCENCAKLWCLDSCCECFGGDLITCC"
     gene            complement(7500..8339)
                     /locus_tag="KRL67_001603"
     CDS             complement(7500..8339)
                     /locus_tag="KRL67_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH1522490.1"
                     /translation="MFGYVKINKMDLTFREYDYYKAYYCGLCKYLKRNHGEISRFSLN
                     YDITFLIVLLTAVYNPESISTEEVCIVNPFKKKKVITNDITEYAASMNILLTYYKLED
                     NLMDDKRIKDKLAYYIYKNKLNLAYEKYPEKAEYIKQQLNELNKLEKDKNINIDEVSS
                     IFGNIMGEVFVYKKDENERNLRMIGFNIGKYIYLLDAYEDLDEDFKKGRYNPFIEYID
                     KNDELKEKVKKIIVTSLGFLARGIDNLNLNKNVGIIENIIYSGVYLRYINILESRGGK
                     NVQ"
     gene            complement(8558..9943)
                     /locus_tag="KRL67_001604"
     CDS             complement(8558..9943)
                     /locus_tag="KRL67_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HBH1522491.1"
                     /translation="MDNLNKKFLMEYIDEIQGELRDISDKIWKNPELQYKEYYASNLQ
                     KECLKKHGFSIEGLEGMDTAFVASYGEGRPVIAVLGEYDALDGLSQKVSTTKEPIVDG
                     GAGHGCGHNLLGTGSIGAVIAVKELIKLKKLKGTIKYYGCPAEEDLSGKVLMIKKGFF
                     DGIDCAFSWHPFDLNTPIRIPTLANYSVKFRYNGISAHAAQAPYNGRSALDAVELMNI
                     GCNYLREHIFDNCRVHYVTTNGGKMPNIVPDFAEVWYYIRGVKMEHVRDVFGRIVDIA
                     KGAALMTGTSMEYDIISGVYDYIPNTILTDILSQNMKLVGVQKYNEDDYYFADRLAET
                     VSIDKRSSVSSVLSGNKDITKMNLHDEVTDDTFTHNNCISLSLDIGDVSYIIPTAQCS
                     CSVWPIGISAHTWQSCASAGSDMGFKAMLLASKSISCSINDVMLDELVINKAKKELKD
                     TVGSFEYIPII"
     gene            complement(10116..10859)
                     /locus_tag="KRL67_001605"
     CDS             complement(10116..10859)
                     /locus_tag="KRL67_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytoplasmic protein"
                     /protein_id="HBH1522492.1"
                     /translation="MKKVLIVGESWVKNITHIKGFDTFVTTHYEEAVKWLKEAIESGG
                     YETVHMPAHVAADSFPYKLEELNEYDCIILSDIGSNTFLLSNSTFIDCNSNPDRLELI
                     KEYVNNGGALIMVGGYMSFTGVDAKARFGETAIKDVLPITMIDKDDRVEKPAGIIPEV
                     IDSEHPVLKGIPTEWPKFLGYNKTVARDNCPVLATIGGEPFVAVGEFGKGKSAIFSSD
                     CAPHWGPKEFTDWKYYNKLWVNMLDWLTC"
     gene            complement(10879..12141)
                     /locus_tag="KRL67_001606"
     CDS             complement(10879..12141)
                     /locus_tag="KRL67_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HBH1522493.1"
                     /translation="MKKILLDTLNSKKQEYIDYLKELVSIKTEDVGHGILGGFEKEGQ
                     EYIEKLANYIGFSVDRQVMSEELIKKAKNIYKEGNLGHNYQDRYNLICKYSDDLPGKT
                     IVFNGHVDTMPPGDISKWKYNPYCATEDNGKLYGLGTADMKSGLIASILAVKLIKDSG
                     LNVPGNVKIMSVVDEEGGGNGTINAVMNGIDGDCCIICEPSEQNLIVAHMGFVFFEVE
                     VKGVSLHCGSKWEGVNAIEKAMLLLQDIKELEHNWLMIYKHPLLPSPTINLGVINGGT
                     AGSTVPDKCVFNLCVHFLPNIMSYEQVVNDVTNVIMIRANGDLWLKDNKPNINVYQSG
                     LGFEMDKDSDFVVNAHKILEETLGKKLEIKGSTAGNDARLMKNLAEMPTLILGPGSIE
                     QCHSIDEYVEIKEYLDSILIYASLILNL"
     gene            complement(12324..13322)
                     /locus_tag="KRL67_001607"
     CDS             complement(12324..13322)
                     /locus_tag="KRL67_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter permease"
                     /protein_id="HBH1522494.1"
                     /translation="MSSITKHKEEKSSPLLTKLTSAFRDQGSAAVGLVIIFIIMSVAS
                     SNFLTLDNLINVGRQISINAILAVGMTFVIITGGIDLSVGAVIALVGTFWATTVVNYN
                     APIWVGMILALAIGTILGVIKGAIISTQKLPPFIVTLAMLTIISGASFVFTGGRPISV
                     NTDAFKMLGRGYIGPIPIPVIIMIIVVIAGHFLLKRTNFGRHVHAVGGNEEGARLCGV
                     KVNKVIVKVYALAGLLTALAGIILSSRLASGSPTVGDGAELDAIAAVVLGGTNMMGGS
                     GSIVGTCIGVGIIGILGNGLNLLSVSSYNQMIIKGLVMLFAIWINNIKLKKSAKSK"
     gene            complement(13306..14817)
                     /locus_tag="KRL67_001608"
     CDS             complement(13306..14817)
                     /locus_tag="KRL67_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HBH1522495.1"
                     /translation="MSNIILKLSNIAKEFPGVRALDNVNFELFHGEVHALLGENGAGK
                     STMIKILTGAHSKTSGKFIFEGKEIENISPDISKKIGINAIYQELTVFDELTVAQNIF
                     MGKEINGKVLTNDKKMNEEAKKIFDNMGIDINPNSLVKELSIAQKQMVEIARVLSSET
                     KVLIMDEPTSSISKKETEILFRLINDLKESGVSIIYISHRMEELFEICDRITIMRDGK
                     TISTLNTKDVPSEEELVNLMIDRKLDQFFPKRKVEIKEEIMRVENLTKNNVFNDVSFN
                     IRKGEILGIGGLVGSKRSEIVEAIFGLKTYDSGKIYLNNEEVKFKTPSDAIENGLGLI
                     TEDRKGTGLFLQMSVKENTTMAGLKKISKFKSIIDRKKEKEILEKYIEALKIKTPHMN
                     QVIQSLSGGNQQKAIIARWLLLQPDILIMDEPTRGIDVNAKAEIYNLMGDLVESGVSI
                     IMISSEIPELISMSDRIMVMREGHISGFLEGEEMVENNVLKLAFGGKINEFNN"
     gene            complement(14871..15833)
                     /locus_tag="KRL67_001609"
     CDS             complement(14871..15833)
                     /locus_tag="KRL67_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HBH1522496.1"
                     /translation="MRVRKILSLGIAGILAIGMLTGCSMKGPSKSDNKGGSDKKDLTI
                     GVSTITLQHQFFIDIDEGIKEKAKELGVKVIVNDPDQDVAKQTSAIEDFIQQNVDGMI
                     VLGTDNSAIVPAVEGAFEKMPVVTVDAVLNTENITSYVGTVSYDAGKKLGEYTKKYID
                     EKLGGKSEIAIVTDLKSQIQMQRIDGFKDALKGSANVKILNSQPGYDREESLNTVENL
                     IQSNPDVDIIYATAENSVLGAKAALESAKNKDVKIVGFDLTEEASSGITDGTILAMIQ
                     QQPKEMGRLAVEAVVKAIKGEKVEKNIPVPALLYDKENIKDFKN"
     gene            complement(15881..16771)
                     /gene="rbsK"
                     /locus_tag="KRL67_001610"
     CDS             complement(15881..16771)
                     /gene="rbsK"
                     /locus_tag="KRL67_001610"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HBH1522497.1"
                     /translation="MGNIVVIGSVNMDMVCSVDKRPEKGETVLGNSFFTSPGGKGANQ
                     AISASKLGANVKMISCIGKDGLGEELIRNFRANKVDYSLVSRNNHKSSGVAVITLCEN
                     DNSIVVVPGTNELVDIELIKKNEEEIKNADIVLLQLEIPLKTINYVVNFCFENRVKVL
                     LNPAPAVKLNEDIIEKVTYLTPNEHEYKIVFDTNEGIEEVLKKYPNKLIITEGKNGAR
                     FYDGEEIKHVSCISVDVQDTTGAGDTFNGALAVAITEGKNLYTSVEYAVVVSGLSVTK
                     LGAQSGMPYKEDVEKYLNNK"
     gene            complement(16813..17826)
                     /locus_tag="KRL67_001611"
     CDS             complement(16813..17826)
                     /locus_tag="KRL67_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBH1522498.1"
                     /translation="MSITIKEIGELAGVSKTTVSKVINNKDENISQATREKILKIMKE
                     KNYVPNKLAQSLVTKKTNTIGLLIPDIRNPFFTDVSRGVEDKANEEGYNIILCNTDED
                     AKKEYEGIRTLSERMIDGIIFAASSNTNWKEANYKDIKIPTLLIDKKISMNKETLKGI
                     VKINNFEGAYIATKHLLDIGNKKIIYLSGPLQNEIAVDRLEGYKKALIECNLSYNPAY
                     VFEGKYKIEWGQEFIKNLEKIDFDAIFCANDLIAIGVIRGLKERGLSIPNDVSVVGFD
                     DIQTSSLISPSLTTVKQPSYDIGYKASEILINCLRGDKKESFDELIFKPELVIRDSTK
                     ESE"
     gene            complement(18052..19032)
                     /gene="bioB"
                     /locus_tag="KRL67_001612"
     CDS             complement(18052..19032)
                     /gene="bioB"
                     /locus_tag="KRL67_001612"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HBH1522499.1"
                     /translation="MEKYTIKLKNKVLRGKEISYEEALNLISLDTNNKNDFDTLLKSA
                     NEIREYFMGRKADLCTIMNAKSGKCSEDCKFCAQSSHYKTGVEEYSLLDYNEILNRAK
                     EMESKGVHRFSLVTSGKGMSGKEFNDILNIYEGLRKNTNLKLCASLGIIDYEKAKMLK
                     SAGVTTYHHNVETCRDNFHNICTTHTYKDRIKTIKDAKKAGLDVCVGGIIGMNESEEQ
                     RLKMAFEIRELNVKSFPINILNPIKNTPMENYDVLEPMEILKTTAVFRFIIPNVYIRY
                     AGGRLSLKGYDKVGFNGGVNSAIVGDYLTTVGSGIENDKKMIIEQGFELY"
     gene            complement(19238..19501)
                     /locus_tag="KRL67_001613"
     CDS             complement(19238..19501)
                     /locus_tag="KRL67_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH1522500.1"
                     /translation="MKIYYEKENSKKANVNNIPSVFAFNFLRNFLTSKEYEETRKEYF
                     INNLTKSQVNQAMKDLKWLFREYEGLEVITMESLDGVKTRIVL"
     gene            complement(19571..20644)
                     /locus_tag="KRL67_001614"
     CDS             complement(19571..20644)
                     /locus_tag="KRL67_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HBH1522501.1"
                     /translation="MEKSKVYFCDLYSNSQNKNVPNNVRRLFDKAGFKDLIEKNDQVA
                     IKLHFGEKGNTTYMSPVAVRQVVDKVKDCEGKPFLTDTNTLYTGSRTNSVDHLTTAIE
                     NGFAYAVVNAPVIIADGLYSRNYENIKIDKKHFESVKIGGEIYNSSAMIVMSHFKGHE
                     AAGFGGALKNLAMGCASAAGKQMQHSDVTPVVKEKKCVGCGKCVNSCPTKAISIVDKK
                     AVIDSDVCYGCGECPTVCPTRAVTIQWESDSDVFVEKMAEYAYGAVSNKKDKVGYITF
                     VMNVTPLCDCVPWSGRPIAHDIGILASTDPVAIEQACYDLICKEMGHDVFKHEHPHVN
                     GTRIIDYACEMGMGSKEYELIKL"
     gene            complement(21113..21871)
                     /locus_tag="KRL67_001615"
     CDS             complement(21113..21871)
                     /locus_tag="KRL67_001615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBH1522502.1"
                     /translation="MREYIAFTKKEFKENIRNYKLLSLTILFLVFGIMSPLSAKFMPD
                     LIAHFAPTLKVTTAPTALDSWTQFSGNISGLGTSLTLIVFCNILSNEYSKGTLVIMLT
                     KGLSRSSVVLSKFSIAVIIMTIGFWLSFLCTYGYTMYFWPTANLNHIIFSAFNLWLIS
                     IMYISILILGCVLFRPAFASVLLVLVATVILSLISIPKQIAPYTPNFILSKNIDLVSG
                     KVIPPEFIIPIIVTIVISIVCLLLAVVLFNKKAV"
     gene            complement(21868..22785)
                     /locus_tag="KRL67_001616"
     CDS             complement(21868..22785)
                     /locus_tag="KRL67_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBH1522503.1"
                     /translation="MNIVSINGLSKGFGNRKIIDNLNFTVPEGSVFGFVGKNGAGKTT
                     TMKMVLGLLEPDSGTIDVCGEKVTYGRTSSNRHVGYLPDVPEFYNYMRPFEYLSLCGE
                     ITGLSKNEIKIRSEELLSLVGLGNEKRRIGGFSRGMKQRLGIAQALLSRPKLLICDEP
                     TSALDPVGRKEILDIMLKIKDSTTVIFSTHILSDVERICDHVAILNKGSIALSGTLSE
                     IKSMHGKDRLLLEFASNDEIQKFKSSDGIKSLLKDSEETNMEIVLHGKDIKAIQKTVI
                     STLAEMNLCPVKMELIELSLENLFLEVVK"
     gene            complement(22785..22985)
                     /locus_tag="KRL67_001617"
     CDS             complement(22785..22985)
                     /locus_tag="KRL67_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLDc_N domain-containing protein"
                     /protein_id="HBH1522504.1"
                     /translation="MENLMEYLPLLVPVIILDLILIITALVHVLRHPNYKIGNKAIWI
                     IVVLFISLIGPILYFTIGRGEE"
     gene            complement(22990..24084)
                     /locus_tag="KRL67_001618"
     CDS             complement(22990..24084)
                     /locus_tag="KRL67_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBH1522505.1"
                     /translation="MNELNIAKTLILKRKEKGITQDELANYIGVSKASVSKWETGQSY
                     PDITFLPQLATYFNITVDELICYEPQMMKEDINKLYNKLCKDFTVKSFDEVMDEIREI
                     IKKYYSCFPLIFRMGLLIVNHYDIVDEKKRELLISEALEIFIRIQETCNDIDICRQAK
                     SMEATCYILLNQPIQVIDLLQNSNFPIINESILLAQGQMMNGQIDEARETFQLGAYQN
                     LISLVQNLVGILQNADKLQMKEIERRILAISDIFELDTLSPAIMLSAYLTQVQINLIH
                     GDNEGAIKSLRKYVDLATRDIYPIIIHGDDFFNKLDRWISEIGTGITRDDTIVKAGIV
                     DAIKNNPMFSVLSENKEYKFLIEKLSLLEE"
BASE COUNT         7843 a         4411 c         2543 g         9430 t
ORIGIN      
        1 taaacataat gtataatgaa tatagattta tatatacaaa aacgctccaa taaaaaggta
       61 ccaataaagt taaaagaaac ttattgatac cttttattat tatatacttc aatatataat
      121 tataagtttt ttgaatagta tggatttaca tttatccctt atctcatagc tctcatagca
      181 tttactactg ctataagtgc cacacctaca tcagcaaata tagcttccca catagtagct
      241 actcctccag cacctagtat cataactata atttttactc ctagagcaaa tattatgttt
      301 tgccacacta tcttattagt cttgtttgct atttcaattg ctttagatat cttgctaggc
      361 tcatctgtca taagcactac atctgcagct tcaatagcag catctgaacc taagcctccc
      421 atagctatac caacatcagc acgagctaat actggagcat catttattcc atcacctaca
      481 aatgctattt tctctttttc agttcttcct tcatataaat cttctaatct atctaccttt
      541 tcattaggaa gtaaatttga gtaaactgta tccaattcta attcttgagc aatatttttt
      601 gctactttct cattatctcc agttaacatt acaacttctt ttacccctat tgcttttaag
      661 cttcttatgg catttttact atcttctttt acttcatcag atataactat ataacctcta
      721 tatactttat caacagcaat atagactact gtacctactt ctttagctga tgaataagat
      781 atattttctt ttttcataag tttctcatta cctgctaata taaaattgcc attatgcttt
      841 actctaattc cataagctgc tatttcctca taactgtcta ttgtatctaa atcaattttt
      901 ttattatagt aacttaatat agactttgct attggatgat ttgaatttac ctccgcaata
      961 gctgcatatt ctaataattc ttcttctgat ataccttcag gatttaactt agttacattg
     1021 aacacacctt tagtaagtgt tcctgtttta tcaaatacta ctgtatctac acttcttaaa
     1081 gcttctaaat agttacttcc tttaataagg ataccatttt tagatgcaaa cccaattcca
     1141 ctaaagaaac taagtggtat tgataaaact aatgcacaag ggcaagaaac gactaagaat
     1201 atcaatcctc tatataacca atcactaaat acctcacctg agattacaag tggtggcaca
     1261 aatgctatta ataaagcagc tattactact attggagtat agtatcttga aaacttagat
     1321 ataaagtttt ctgtctttga ctttttaatg ctagaatttt caacaaggtc aagaatctta
     1381 gaaactgttg attctccaaa actctttgta acttcaatac tcaataaagc atttttattt
     1441 ataacacctg ataaaatttc gtctcctttt tcaacttctc tcagtactga ctctcctgta
     1501 agtgcagaag tatctagcat agaaattcca tccactacta caccatctaa tggtactttc
     1561 tcaccaggtt ttactactat tatatcgcct acatttactt cttctggtga caccacctta
     1621 acttcagaat taacttttaa atttgcatag tctggtctta tttgcataag tgaagttata
     1681 gactttcttg atttaccaac tgcaacacct tgtaaatact ctcctacttt atagaaaagc
     1741 ataaccccta ctgcttctga taactcccct attgctaatg ctcctactgt tgctagagcc
     1801 atcaaaaagt tctcatcaaa tactcttcct tttgatatat ttcttattgc tcttaaaagc
     1861 acatcgccac ctactattac ataggcaact ataaatacta tattgctaaa ctgactttca
     1921 taacctgtag ctgtttgata tataccaaat atatatacaa gtgctccaat tataagtgga
     1981 attaattctt ttttatttgt atcatttaca gctcctggtt tttcagtagt tctccctttt
     2041 actttgttag tagactctac ttgtatatca agccctggct ctgttgaatt tattatttct
     2101 tttattttat ttataacatc atccgcttca aagttattac ttatatttac tgtaagtttt
     2161 ttatttacaa agtttaaatt agaagattca acttctttta atttagaaac tttgttatta
     2221 atttcctctg cacaatgtgc acagtttaat cctcctaata taagctcttt tttaattgat
     2281 attttactag atgcattttc tttagaactt atttgtatat caagccctgg ttctgttgaa
     2341 tctattatat ctattatctt ttctatagta gtatcctcat taaagctttc ctttatattt
     2401 actgtaagtt ttttatttat aaaatttaaa ttagaagatt taacttcttg tagtttagaa
     2461 actttattat taatctcttc tgcacagtga gcacaattta atcctcctag gataaattct
     2521 tttttaatta aattatttgc ttccatatat attcactcct ctcatttatc atttataagt
     2581 ctctctaata tgatttaatc cacaatcaaa tatctgactt atatgactat catctaatga
     2641 gtaatacact gtttttcctt ctcttctaaa ctttactaat ctagcctgct ttaatactct
     2701 aagttgatga gatattgcag agtgagtcat atttaataaa ctcgctatat cacatacaca
     2761 catttcattt gcaaataatg catataatat ctttactcta gtagtatccc caaatacttt
     2821 aaataattct gctaaatcat ataacatctc ttcatctggc atagttgatt tagcctctgt
     2881 tactatttct tcatgtacta tattacaatt acaatcattt atctcttctc tcatttttaa
     2941 tcctccaatt attttaatat atgaataact gttcatatgt tcattatatg ctatcatttc
     3001 taaatttgca acaataattt ttattaattt aatataatta gaatgcacat attataaact
     3061 tttttaatat atatattatt agaataagtt tttgtaaact tttttatagt tttctaggag
     3121 gtgcaagttt aatatgagta acaagaaaaa aaaggattta gatactagtt acatgcctta
     3181 cttagaccca gcaaactatg cagattatat tactaatgaa gaaattcccc aagatgaatt
     3241 gagaaatgac cctcaaattt ctaatggtta tacaaaaata cctaataacc agaacataaa
     3301 taacctatat cctaataatg taaatcatta tcctaaaagt agtaattctc caaacaactt
     3361 gcctaacaca aataatacat ccggaaatat gaattacaat acatctgcta caaataatat
     3421 gcctaataac atgaataata atacgggtat gcctaataat atgtctaaca atatgaatag
     3481 taatatgggt atgcctaaca acatgaatag taatatgggt atgcctaaca acatgaatag
     3541 taacatgggt atgcctaaca atatgcctaa taatatgaat agtaacatgg gtatgcctaa
     3601 taacatgaat agtaatatgg gtatacctaa caatatgtct aataatatga atagtaatat
     3661 gggtatgcct aacaatatgt ctggtaatat gaataatatg tatggtaata caagtatgcc
     3721 taataacatg aacagtaata tggggtataa tatgaacaat actatgggta ttcctaactt
     3781 ctcatgtaat caaaatatgc ctcctaatgt acttatgatg ataccaggag ttatatgtca
     3841 taatacaatg caaggtatgc ctgtagttat gcctagtact atgcctccta atatatatcc
     3901 tactccttat ggttctagta atatgtctat acaaggtata ccacagacta ctaatattga
     3961 agaatttgat gaggaagaaa tgtaatatat atttttttaa gtaagttgta taaaacatta
     4021 ataattgacc ataataataa atgtattcct cataatattc ccccaatatt attataggta
     4081 tagaaagttg actgaatatg cgcttattcg gtcaacagat attaacataa tctaataaga
     4141 taaacataga aaaagtcaga taaaatttaa ttttatctga ctttttcatt taaaaatgct
     4201 ctcttgtgtt ggtaggtcac aatagtatgg tactcgtatt tgagactaat attggcaaac
     4261 aaaataaaga aaagtataaa ttttttataa taaactgtat aaaattttaa taattgacca
     4321 caataataaa tgtattcctc ataatattcc cccaatatta tagaaataaa tttaagcgag
     4381 attaaattca gttaatctcg cttaaataat gtctaaattt attattccaa aaaatcatca
     4441 aaatctaatg aacctgattc tgcaaagtca tataatgcat tttgttcaaa gaaaaatttc
     4501 tcttcaaatt taatatagtc actataatta gattgactta tttcattgat ataattgcaa
     4561 tcattttcgt tataagtacc aataaaaaac ttatttccca ttaagtaata tatagctttc
     4621 atatcattat ttattatata tacattttct tttacatggt cattattaaa attaaatcta
     4681 gtaaaaaata taccttcatt catattactt tttaacaaaa atggattttc tctataaaat
     4741 actcctgcaa attcttcagg tgaatttaca ctgtatacac ttacaaattc acttttagaa
     4801 atctcatcaa atacctcaaa atcatacata ggttctttat ctgtcactaa tagagagtta
     4861 atcctaaaac cagtttcata acacttactt atgcaaaaag caattttaca tatatagtat
     4921 ccatcattat cttcatataa agcttcactt atatatctgc cttgaccttt atcacttacc
     4981 acatttttaa gtaatgttcc atttatatta gtaatatgca tatttgcaat ctcgtcactt
     5041 ccagaaaagt actttaaact ttctctatcc catgctataa atctcatagt catataattt
     5101 ataaattcaa cttcttcatc aactctttta catattttat tgtataattt ttctatagtt
     5161 aaatctgttt tatagttttt tataatatca atatactcac atttatctcc tggcaatagt
     5221 ttatcataat aaagattctt attaccaaaa taagatacta aaaataaaga ttcatccttc
     5281 gttaattcaa ctctatctga accaaatcct cccatcaatc tttcttcttc catataagct
     5341 tcttcttgtg ttgggttatt taaactcaca taatctgcta atcctaatcc ctctgcatct
     5401 aataaaaaat attgacaaaa gtgattgttt tcatcatcta tccaatttat tataagacct
     5461 aaacttccca taagtctaga atttgtagcc tttgcaaaat taaaattcat atagtcacat
     5521 cctttaaact ctaaaatact tgttttttaa tatataaatt attatacata tattttaaca
     5581 acttagatac aatttataat ataatataag gaaatgatta attgaggtgg tcttatgaaa
     5641 aatatagcag ctttttttga tatagatggt actctttata gagactctct tatggttgaa
     5701 cattttaaga aattaattaa atacgatata attgaccaaa aaaattggta taaacatgct
     5761 agagacactt ttatggattg ggataaaaga caaggtaatt atgatgatta tctattagaa
     5821 atatgtgacc tttatgttga ttcattaaaa ggattagata aaacctgtat caactttaca
     5881 agtgaccaag taattaaatt aaaagcagat agagtctata aatacacacg ttcaaggatt
     5941 gagtggcatc taaatcaagg acacatagtt atatttatat ctggtagccc tggatttctt
     6001 gttgaaaaaa tggcaaaaaa atataatgta accgattttc taggaagtga ttatgtcttt
     6061 gaaaataata tatttacagg cacagttatt ccaatgtggg attctataag taagaataat
     6121 gctataaacg attttgttgt aaagtatgat ttagatttat caaactcgta tgcctatgga
     6181 gatactaatg gtgatataaa tatgctaaaa agagttggaa atccaatagc aataaatcca
     6241 acaaaggaac ttttatctca aatatcactt gatgaggaaa tttctaaaaa tgttgagata
     6301 atagtagaaa gaaaagatat tatttattct ctatctacag atgtaaatat agtggatatt
     6361 taatttatac tatctatttt aaaaacttat attatatcta atatttatat ctaacatata
     6421 aaagctttga ctaaaaatca aagcttttat atgttgcact aagaatatat taaatttagc
     6481 tttatgtttt aatgtatatt catatatata atttacactt atccaaacta caatttcatt
     6541 aaatttaaat ataaaaatca actattattt tttaaatagc atctttctta acctattatt
     6601 gtaataacct ataaaagaac tgtaaacata gtcatatact ataaaggcta tctcaaatga
     6661 acctataata aatatgttta ttggaatata aactattgtt cttaataaaa aatacaatat
     6721 aagtatcatt atatttgcaa atactaattt cataaaatac tcaatatata tagacttatc
     6781 tttttctata agatatttta caataccata tattccaaag gtaagtgaat atacaatcca
     6841 ttgagactta cttgccatca ctataaatcc taaaactata gaacctatat aaaaagcaat
     6901 tccactctta aagccccatt ccattattac aattgatact aaaagtgatg ctaaccccat
     6961 aataaacaat gtatttacag gaattatatt tgtaagagtc aacaatatta catttaatgc
     7021 taataaaact cctccataag caacttttat actcatattt atcatcctaa caacaagtta
     7081 ttaaatctcc accaaaacat tcacaacatg aatctaaaca ccaaagttta gcacaatttt
     7141 cacaacaatc gtcacctaga caacaacagc aatcatcaca gcaacagcaa caacatccat
     7201 ctaaatcatt acgtcttctt ctattatagt tgtcagccct attgctataa tcatttctat
     7261 attgtgtata acttctaagt gcatcactat attcactatt ttcaggctcc ataaattttg
     7321 ctctttttaa gaagtcttca ccttcttcat aatatcctat gttcattgca gaaaagccag
     7381 ttaaataata ccactctgca catttgtcag ttaaagtttt taaaaaatca tatgcttttt
     7441 taaattcact attttctatg aatgctcttg ctctcataaa attttcatta ttataagtat
     7501 tactgtacat tctttccacc tctactttct aatatattaa tatatcttaa ataaacacca
     7561 gaataaatta tattttctat tattccaaca tttttattta gatttagatt atcaattccc
     7621 ctagctaaaa accctaatga tgttactatt atttttttta ctttttcttt taactcatca
     7681 tttttatcaa tatactctat aaatggatta tatcttcctt tcttaaaatc ttcatctaaa
     7741 tcttcatagg catctagtag atatatatac ttacctatat taaaacctat cattcttaag
     7801 tttctctcat tttcatcttt tttatagaca aatacttcac ccattatatt tccaaatata
     7861 ctagatactt catctatatt tatatttttg tctttttcta acttatttaa ttcatttagt
     7921 tgttgtttta tatactctgc tttttcagga tacttttcat atgctaaatt taatttgttt
     7981 ttatatatat aatatgccaa tttatctttt atgcgtttat catccattaa attatcttcc
     8041 aatttataat acgttaaaag aatattcata cttgctgcat attctgtaat atcatttgta
     8101 attacctttt tctttttaaa tggatttact atacacactt cctctgtgct tatagactct
     8161 gggttatata ctgctgttaa taaaacaatt aaaaatgtta tatcataatt caaagaaaac
     8221 ctagagattt ctccatgatt tctttttaag tatttacata gtccacaata gtaagctttg
     8281 taataatcat attctctaaa tgttaaatcc attttgttta ttttaacata tccaaacata
     8341 aatactcctt tttgtactat tttttattta gattatatat agtataacac tttttatcaa
     8401 gattatataa tttacagtat tctaagaaac ttaaaaaatt ccttctaatc attgtatctt
     8461 aaatttaaaa taattagaag gttgggttaa aaataatatt aaattatata cttatattaa
     8521 ctcatacaag ttagttatgc ttatatcaat ttaagtttta aataattggt atatattcaa
     8581 aactacctac agtatccttc aattcctttt ttgctttatt tataaccaat tcatcaagca
     8641 ttacatcatt tattgaacat gatatagatt tagatgccaa taacattgct ttaaatccca
     8701 tatcagaacc tgcactggca caagattgcc atgtatgggc agaaatacca ataggccaaa
     8761 ctgaacaaga acattgtgct gtaggaatta tgtagctaac atctccaata tccaatgata
     8821 atgatataca gttattatga gtaaatgtat catctgtaac ctcatcatgt aaattcatct
     8881 tagtaatatc cttatttcca cttaatacac tagaaacact acttctttta tctatagaca
     8941 ctgtctcagc taatctatct gcaaaatagt agtcatcttc attatatttt tgcacaccta
     9001 ctaatttcat attttgtgat agaatatctg ttaatattgt atttggaata tagtcatata
     9061 ctccacttat aatgtcatat tccatactag taccagtcat taatgctgct ccttttgcta
     9121 tatcaacaat cctaccaaat acatctctta catgctccat tttaactcct cttatgtaat
     9181 accacacttc cgcaaaatct ggaactatat ttggcatttt tccaccatta gtagttacat
     9241 agtgtactct acagttatca aatatatgtt ctctcaaata attacaacct atattcataa
     9301 gttctactgc atcaagtgca cttcttccgt tataaggagc ttgtgctgca tgagcactaa
     9361 tcccattata tctaaatttc acagagtaat tagcgagtgt tgggattctt atcggagtat
     9421 ttagatcaaa tggatgccaa ctaaaagcac aatcaattcc atcaaaaaaa ccttttttta
     9481 tcatcaatac ttttccagat aaatcttcct ctgctggaca tccatagtat tttatagtcc
     9541 ctttcagctt ttttagtttg attaattcct ttactgcaat tacagctcct attgaacctg
     9601 tacccagtaa gttatgtcca catccatgac ctgcaccacc atctacaata ggttctttag
     9661 tagtggatac tttttgagat aaaccatcta aagcatcata ttctcccaaa actgctataa
     9721 ctggtctacc ttctccataa ctagccacaa atgctgtatc cataccttct aatccctcta
     9781 tactaaaacc atgttttttt aaacattctt tttgtaaatt tgaagcatag tattctttat
     9841 actgaagttc tggatttttc catatcttat ctgatatatc tcttaattct ccctgtatct
     9901 catctatata ttccatcaaa aactttttat ttaaattatc catattaatt atataaacct
     9961 ccctgtttct aataacttat cgattatcag ctaaaatgac taattttaac tgatttaaaa
    10021 atattttcta ccatacaacc attttataca ataaaacctc ttgacttgta aatttcattt
    10081 tatcaagtaa gaggttttca agttttataa aatttttagc atgttaacca atcaagcata
    10141 ttaacccata atttattata gtatttccaa tcagtaaatt cttttgggcc ccagtgaggt
    10201 gcacagtcag aactaaagat agcagatttt ccttttccaa actcgccaac agctacaaat
    10261 ggttctcctc caatagttgc taaaacagga caattatctc tagctactgt tttgttatat
    10321 cctaaaaact ttggccattc tgttggtata ccttttaata ctggatgttc agagtcaata
    10381 acttctggaa ttattcctgc tggtttttca actctatcat ctttatctat cattgttatt
    10441 ggtaatacat ctttgatagc agtttctcca aatctagctt ttgcatctac tcctgtaaat
    10501 gacatatatc cacctaccat gattaacgcc ccaccattat ttacatactc tttgattagt
    10561 tcaagtctat ctggattact attgcaatct ataaaagtac tattagataa taagaatgta
    10621 ttagaaccta tatcagataa tattatacag tcatactcat ttaactcttc taatttatat
    10681 gggaaactat ctgctgctac atgtgcaggc atatgaactg tctcatatcc tccagattct
    10741 attgcttctt tcaaccactt tacagcttct tcataatgag ttgtaacaaa tgtatcaaat
    10801 ccttttatat gagttatatt ttttacccaa gattccccta ctattaatac ttttttcatt
    10861 tatatattcc tcctactttt ataagtttaa tattagactt gcatatataa gtatactatc
    10921 taaatactcc ttaatttcaa catattcatc aattgaatga cattgttcta ttgaacctgg
    10981 tcctaatata agtgttggca tttcagctaa atttttcatt aatctcgcat cattgccagc
    11041 tgttgaacct tttatttcta attttttacc tagagtttct tctagtattt tatgtgcatt
    11101 cactacaaaa tcagaatcct tgtccatctc aaaacctaac cctgattgat atacgtttat
    11161 atttggttta ttatctttaa gccataaatc tccattagcc cttatcatta tgacatttgt
    11221 tacatcattt acaacttgtt cataactcat tatatttggc aagaagtgta cacataagtt
    11281 aaatacacac ttatctggca cagtagagcc tgctgttcca ccatttatta cacctaaatt
    11341 tattgtaggt gaaggtaata gtggatgctt atatatcata agccagttat gttccaactc
    11401 ttttatatct tgaagcaaca acatagcctt ctcaattgca tttacaccct cccacttact
    11461 accacagtga agagacacac ctttaacctc aacttcaaag aatacaaatc ccatatgtgc
    11521 aactattaag ttttgttctg atggttcaca aattatgcaa caatctccat caataccatt
    11581 cataactgca tttatggtac catttccgcc accttcttca tctactactg acattatttt
    11641 tacattacca ggtacattta gaccactatc ttttataagc tttacagcta atattgatgc
    11701 tataagccca gatttcatat cagcagttcc aagaccataa agttttccat tatcttctgt
    11761 agcacaatat ggattatatt tccacttact tatatcacct ggtggcatag tgtctacatg
    11821 accattaaat acaattgtct taccaggtaa atcatcacta tacttacaaa ttaaattata
    11881 tctatcttga taattatgac ctagatttcc ttctttatat atattttttg cttttttaat
    11941 taattcttcg ctcattactt gtctatctac actaaaacct atgtaatttg caagtttttc
    12001 aatatattcc tgtccttctt tttcaaatcc acctagtatt ccatgaccga catcttcagt
    12061 ctttatgctt acaagttctt ttaaataatc tatatattct tgctttttac tatttaatgt
    12121 atctaagaga atttttttca tatttcctcc taagatttat ttttacattt aaaatttcaa
    12181 gtctaatctc atctatttaa gccatagtaa actttttcac ttagcatatt cccatttaat
    12241 gtataaagat atatgtaaat taaagtataa aaatatatac aaaacttaat aatttttata
    12301 gtttataagc taaaatttaa ttactattta gatttagctg atttttttaa ttttatatta
    12361 tttatccata tagcaaataa cattaccaaa ccttttataa tcatttgatt atatgaagat
    12421 acacttaata agtttaatcc atttcctaat attcctatta ttccaacacc tatacatgtc
    12481 cctactatag aaccagagcc acccatcata ttagtgccac caagcacaac ggctgctata
    12541 gcatccaatt ctgctccatc tccaacagtt ggtgaacctg atgccaaacg tgatgataat
    12601 attatcccag ctaatgctgt tagtagtcca gctagtgcgt atacttttac tataacttta
    12661 tttactttta ctccacataa tctagcaccc tcttcatttc cacctactgc atgtacatga
    12721 cgtccaaaat tagttctttt taatagaaaa tgtcctgcta ttacaactat aatcataatt
    12781 ataactggta ttggtattgg tccaatatat cctctaccga gcatcttaaa tgcatctgta
    12841 tttactgata taggtcttcc tcctgtaaat acaaagcttg ccccacttat tatcgttaac
    12901 atggctaaag ttacaatgaa tggaggtagt ttttgtgttg aaataattgc accttttatg
    12961 actcctaata ttgtccctat tgctaatgca agtatcatac ctacccatat aggtgcatta
    13021 taattcacta ctgttgtagc ccaaaatgta cctactaaag caattactgc tcctacactt
    13081 aaatctattc ctcctgtaat tattacaaat gtcataccta ctgcaagtat tgcatttatt
    13141 gatatttgtc tcccaacatt aattaaatta tctaaagtta aaaagttaga tgaagctaca
    13201 ctcattatta taaaaattat tacaagtcct acagctgcac tcccttggtc tctaaatgca
    13261 cttgttaatt ttgtcaataa tggagaagat ttttcttctt tatgcttagt tattgaactc
    13321 attaatttta cctccgaaag ccaattttaa tacattattt tcaaccattt cttcaccttc
    13381 taaaaatcca cttatatgac cttctctcat aaccattatt ctgtcactca ttgatattaa
    13441 ttctggtatt tcagaagata tcattattat acttacacca ctctccacta aatctcccat
    13501 tagattgtat atctctgcct tagcatttac atctatacct ctggttggtt catccataat
    13561 taatatatct ggttgtaata aaagccatct tgctattata gctttttgtt ggtttccacc
    13621 acttaaactt tgtataactt gattcatatg tggtgttttt atctttaaag cttctatgta
    13681 tttttctaaa atctcttttt ccttttttct atctataatt gatttaaact tagatatctt
    13741 ttttaaccca gccatagttg tgttttcttt gacactcatt tgtaaaaaca atccagtacc
    13801 ttttctatct tctgttatta atcctagtcc attttcgatt gcatctgatg gagttttaaa
    13861 ctttacttct tcattgttta aatatatttt cccagaatca tatgttttaa gtccaaatat
    13921 agcttctact atttcacttc tcttacttcc aactagtcca cctattccca atatttcacc
    13981 ttttcttata ttaaagctaa catcattaaa aacattgttc ttagttagat tttccactcg
    14041 cataatttct tcttttattt ctacttttct tttcggaaaa aattggtcta gctttctatc
    14101 tatcattaaa ttaactaatt cttcctcaga tggtacatct ttggtattca aagtcgaaat
    14161 cgttttccca tctcgcatta tagttattct gtcacaaatc tcaaagagtt cttccatcct
    14221 atgggatata tatattatac tgactccact ctcttttaaa tcatttataa gtctaaaaag
    14281 tatttcagtc tcttttttgc ttattgaaga agtaggttca tccattatta atactttagt
    14341 ttctgatgat aaaactcttg ctatttctac catttgtttt tgagctatac taagctcttt
    14401 gactaaacta tttggattta tatctattcc catattatcg aaaatctttt tagcctcttc
    14461 attcattttt ttgtcattgg ttaagacttt gccatttatt tcttttccca taaaaatatt
    14521 ttgtgctact gttaattcgt caaaaactgt tagttcttga tatatggcat ttatacctat
    14581 ctttttagag atatctgggg atatattctc tatttctttg ccttcaaaaa taaattttcc
    14641 agaagttttc gaatgtgcac cagttaaaat ctttatcatt gttgatttac cagcaccgtt
    14701 ttcacccagt agtgcatgca cttcgccatg aaataactca aaattaacat tatctaatgc
    14761 tctgacacca ggaaattctt tagcaatatt acttaacttc agaattatat tactcataat
    14821 gacactcctt tttaaatagg atttatttca aataatctca ttttatttta ctaattctta
    14881 aagtctttta tattttcctt atcataaagt aaagctggta ctggtatatt cttttctacc
    14941 ttttcccctt ttatagcttt tactactgct tctacagcta aacgtcccat ctctttaggc
    15001 tgttgttgaa tcatagcaag aatagttcca tctgttattc cacttgatgc ttcttcagtt
    15061 aagtcaaacc ctacaatctt aacatcctta ttttttgcag attcaagtgc agctttagct
    15121 cctagtacac tattttcagc tgttgcatat attatatcta catctgggtt agattgaatt
    15181 aaattttcta ctgtattaag agattcttct ctgtcatatc ctggttgtga atttaatatc
    15241 ttaacatttg ctgaaccttt taacgcatct ttaaatccat ctattctctg catttgtatt
    15301 tgagatttta aatctgtcac tattgctatt tctgactttc cacctaactt ttcatctatg
    15361 tattttttag tgtattcccc taatttttta ccagcatcat aactaactgt tcctacataa
    15421 cttgtaatat tttcagtatt taatacagca tctactgtaa ctactggcat tttttcaaat
    15481 gctccttcta ctgctggtac tatagccgag ttatcagttc caagtactat cattccatct
    15541 acattttgtt gtataaaatc ttctattgct gatgtttgct tagctacatc ttggtctggg
    15601 tcatttacta tgactttcac acctaattct ttagcttttt cttttattcc ctcatcaata
    15661 tctataaaaa attgatgttg aagagttatt gtagatactc caatagttaa atctttcttg
    15721 tctgaaccac ctttattatc tgatttactt ggtcctttca ttgaacaccc tgttaacatt
    15781 cctattgcta gtattccagc aattcctaaa gataaaattt ttctcactct cataattaac
    15841 ctccattttt aatagtttta aatttgaaca aattagattt ttatttatta tttaaatatt
    15901 tttcaacatc ttctttataa ggcataccag attgtgcacc taatttagtt acagaaagac
    15961 ctgatactac tactgcatat tctactgatg tatataaatt tttcccttct gttatagcca
    16021 ctgccaatgc tccattaaat gtatctcctg ctcctgtggt atcttgaaca tcaacactta
    16081 tacaagatac atgcttgatt tcctcaccat cataaaatct agctccattt tttccttctg
    16141 ttattataag tttatttgga tattttttta atacttcttc tatcccttca tttgtgtcaa
    16201 aaactatctt atattcatgt tcatttggtg ttaagtaagt tactttttct attatatctt
    16261 catttagttt tactgctggt gctggattta ataaaacctt aaccctattt tcaaaacaga
    16321 aattcactac ataatttatt gtttttaatg gaatctctaa ttgtagcaat actatatctg
    16381 cattctttat ctcttcttca ttctttttaa ttaattctat atctactagc tcattcgtcc
    16441 ctggtacaac aacaatacta ttatcatttt cacataaggt tataacagca acaccagaac
    16501 ttttgtgatt atttctggat actaagctat aatcaacttt attggctcta aaatttctta
    16561 ttaactcctc tcctaaacca tctttcccta tgcatgatat catttttaca tttgctccta
    16621 gtttagaagc tgagatagct tgattagccc cttttcctcc aggtgatgta aaaaaactat
    16681 tacctaatac tgtttctcct ttttctggtc ttttatctac agaacatacc atgtccatgt
    16741 ttacacttcc tatgacaact atgtttccca ttacattact cctttacata tctatttatg
    16801 tctctcgtgc tgttattcac tttcttttgt tgaatctctt atgactaatt ctggtttgaa
    16861 aatcaattca tcaaacgatt cttttttatc tcctcttaaa caatttatca atatctcact
    16921 ggctttatat cctatatcat aagatggctg ttttactgtg gtaagagaag ggcttattag
    16981 acttgatgtt tgaatatcat caaatcccac aacacttaca tcatttggta tacttaatcc
    17041 tctttcttta agacctctta tgacccctat tgcaatcaaa tcatttgcac aaaatattgc
    17101 atcaaaatct atcttttcta aattttttat aaattcttgt ccccattcaa tcttatattt
    17161 tccttcaaat acataagcag gattgtagct taaattacac tcgattagag cctttttata
    17221 tccttctaat ctatctactg ctatttcatt ttgaagtgga ccacttaggt atattatttt
    17281 cttatttcca atatctaata aatgttttgt agctatatag gctccttcaa agttatttat
    17341 tttaacaatt ccttttaagg tttctttgtt catgcttatc ttcttatcaa tcaacaaagt
    17401 tggtattttt atatctttat aatttgcttc tttccagttt gtatttgaag atgctgcaaa
    17461 tataattcca tctatcatcc tctctgatag tgttcttatt ccttcatatt cttttttagc
    17521 atcttcatct gtattacata aaattatatt gtagccttct tcattagctt tgtcttctac
    17581 acctcttgat acatctgtaa agaatggatt tcgtatatct ggtataagaa gacctatagt
    17641 attagttttt ttagttacta aactttgtgc taatttattt ggtacataat ttttttcttt
    17701 cattattttc aaaatttttt ctcttgttgc ctgacttata ttttcatctt tattattaat
    17761 gacttttgac acagttgttt tagaaacacc agccaattcg ccaatttctt ttatagttat
    17821 actcaagaag ttcaccccca aattttagga aaccgtttta cttaaccggt tacctagata
    17881 ctatcacatc ttttttccct ttgcaaccat tttatataaa ttttgatatt tttccctttt
    17941 ttcaaaatat tgcaacttat aattcaattt taatatttaa attaatctac aaaaaaaaat
    18001 taatatactt ttataattat ctaaaagtat attaattaat ttaagtagta tttaataaag
    18061 ttcaaatcct tgctctataa tcattttttt atcattttca atcccactcc caacagttgt
    18121 caaataatca ccaacaattg cagaatttac tcctccatta aatccaactt tgtcatatcc
    18181 ctttaatgat agccttccac cagcatacct tatgtacaca ttaggaatta taaacctaaa
    18241 tactgctgtt gtttttagta tttccattgg ttctaataca tcataatttt ccattggtgt
    18301 attcttaata ggatttaaaa tgtttattgg aaatgatttt acatttaatt ctcgtatttc
    18361 aaaagccatt tttagtctct gttcttcact ctcattcatt ccaattatac cgcctacaca
    18421 tacatctagc ccagcttttt tagcatcttt tatagttttt attctatctt tatatgtgtg
    18481 tgttgtacat atattatgaa aattatctct acacgtctca acattgtgat gatatgtagt
    18541 cacacctgct gatttcaaca ttttagcttt ctcataatct ataattccta aagatgcaca
    18601 taactttaaa ttagtatttt tcctcaatcc ttcatagata tttaaaatat cattaaactc
    18661 ctttcctgac atacctttac cacttgtaac caatgagaat ctatgcactc ctttactctc
    18721 catctcttta gctctattta agatttcatt gtaatctaat aaggaatatt cttctacacc
    18781 agttttataa tgagatgact gagcacaaaa tttacagtct tcactacatt tgccagattt
    18841 agcattcatt atcgtacata aatctgcttt tcttcccata aaatattctc ttatttcatt
    18901 tgcagatttt aatagagtat caaaatcgtt tttattattt gtatcaagac ttattaaatt
    18961 aagagcttct tcataactta tttcttttcc tctcagtact ttatttttta attttattgt
    19021 gtatttttcc atataccctc ctctttgtca acttactatt tttatacagt ttacttatta
    19081 tatatagtat tgcatatata acatttattg gtcaacttta ttttagtcat aagtttacta
    19141 ttctcaaaaa aatactgctt caaaataata ttagtatata gattatacat attattatta
    19201 aagcagtatt ttttattaga atataattct tactgattta taaaactatc cttgtcttaa
    19261 ctccatccaa gctttccatt gtaataacct ctaagccttc atattctcta aatagccatt
    19321 ttaaatcctt catggcttga ttaacctgac tttttgttag attatttata aaatattctt
    19381 ttctagtttc ctcatactct ttgctagtca aaaagtttct tagaaaatta aacgcaaata
    19441 cacttggaat attgtttaca ttagccttct tgctgttttc cttctcatag taaattttca
    19501 taaatgcata ctcctttgat acttttagat ttctcttaca ataattatat gcatttaagt
    19561 gctactattc ttataatttt attaattcat attctttact tcccataccc atttcacatg
    19621 catagtctat tattctagtt ccatttacat gaggatgttc atgtttgaaa acatcatgac
    19681 ccatttcctt acaaatcaaa tcgtagcatg cttgttcaat agctacaggg tctgttgatg
    19741 ccaatattcc tatatcatgg gcaattggtc tacctgacca aggtacacaa tcacaaagtg
    19801 gagttacatt cataacaaaa gttatatacc ctaccttgtc ttttttattt gatactgctc
    19861 cataagcgta ttctgccatc ttttcgacaa acacatctga gtcactttcc cattgtatag
    19921 ttactgctct agttggacag acagttggac attctccaca tccataacat acatcagaat
    19981 ctataactgc ttttttatcc actatactta tagcttttgt aggacaagaa ttaacacact
    20041 ttccacaacc tacacacttc ttttccttta ctactggagt aacatcagaa tgttgcattt
    20101 gttttcctgc tgcacttgca catcccatag ctaagttttt taatgctcct ccaaaacctg
    20161 ctgcttcatg acctttaaaa tgactcatta ctatcattgc agaagaatta taaatttctc
    20221 cacctatttt tacactttca aaatgttttt tatctatttt tatattctca taatttctac
    20281 tatataatcc atctgcaatt attactggtg catttacaac tgcataagca aatccatttt
    20341 ctatagcagt tgtaagatgg tctacagaat ttgttctact ccctgtatac agtgtatttg
    20401 tatctgttaa aaatggcttt ccttcgcagt ctttaacctt atcaaccact tgtctaactg
    20461 ccacaggact catatatgta gtatttccct tttctccaaa atgaagttta attgcgactt
    20521 ggtcattctt ttctattaaa tccttgaatc ctgctttatc aaataatctt cttacattgt
    20581 ttggtacatt tttattttgc gaatttgagt ataaatcaca aaaatatact tttgactttt
    20641 ccatatctac acccctttaa aatatttact tatactaatt ttatcacttt taaaataatt
    20701 ttttagtata ttttaccaaa attaaagatt cttatcttta tttaatgaaa acaatattat
    20761 tttagtcctt cactacttta gactctatat actaaatatt gtaaataact gacaaacatt
    20821 agattataat ctatatctct ttttttagat agctccttaa ggtaataact taattattat
    20881 aaagtatagt gaggacgact aaaataaaaa agtaagtgcc tcaaaatgaa ttttttagtt
    20941 tataagtcac ttttttgtat aaaatcaaat atgttttata attttaacaa taaaaaagag
    21001 gcttatttct ctattttgag acagcctctt ttttacttaa atacattctt ctacaatact
    21061 aaacacattt ttccataata cagtgatatt tgtatttata ataaaaatat ccttaaactg
    21121 cttttttatt aaacaaaaca acagctaata atagacatac tatcgaaata acaattgtta
    21181 caatgattgg aataataaat tctggaggta taactttacc tgaaactaaa tctatatttt
    21241 ttgataatat gaaattaggc gtataaggtg caatctgttt aggaatagaa atcaaactca
    21301 aaataacagt tgctactaat acaagtaaca cacttgcaaa tgctggtcta aatagtacac
    21361 acccaagaat taatatggaa atatacataa tactaattaa ccataagtta aatgctgaaa
    21421 atataatatg attaagattt gcagttggcc aaaaatacat tgtatatcca taagtacata
    21481 aaaagctcaa ccaaaaacca attgtcataa taatcactgc aatagaaaat ttagacaata
    21541 ctacagatga acgtgataac cctttagtca acataataac gagtgttcct ttagaatatt
    21601 cgtttgataa aatattgcaa aaaacaatta gagttaaact cgttccaaga ccagaaatat
    21661 ttccggaaaa ctgtgtccaa gaatctaaag cagttggtgc agtagtaact tttaaagttg
    21721 gggcaaaatg agcaatcaag tctggcataa attttgcaga aagaggactc attattccaa
    21781 atactaaaaa taaaatagtc aaactaagta atttataatt tcttatgttc tccttaaact
    21841 ctttttttgt aaatgctatg tattctctca ctttactacc tccaaaaata aattttcaag
    21901 tgataattct attagttcca tctttactgg acaaagattc atttctgcga gtgtagatat
    21961 aactgttttt tgtattgctt ttatatcttt accatgcaaa acaatttcca tattagtttc
    22021 ttctgaatct tttagtaagg atttaatacc atcactagac ttaaactttt gaatttcatc
    22081 attacttgca aactccagta aaagtctatc ctttccgtgc atacttttta tttctgaaag
    22141 tgtaccgcta agagcaatgc ttcctttgtt taatatagca acatggtcac agattctttc
    22201 aacatctgat agtatgtgtg ttgaaaatat tacagttgtt gagtctttaa ttttaagcat
    22261 aatatctaat atttcttttc gaccaacagg gtcaagtgca cttgttggtt catcacaaat
    22321 aagtaatttg ggacgtgaaa gcaaagcttg agctatacca agtcgttgct tcattccacg
    22381 agaaaaccca cctattcgtc tcttttcatt accaagcccc acaagtgata acaactcttc
    22441 actccttatc tttatttcat tttttgaaag ccctgtaatt tcaccacaaa gagaaagata
    22501 ttcaaatggt ctcatatagt tgtaaaactc tggaacatca ggcagataac ctacatgacg
    22561 attagaactt gttcttccat aggtcacttt ctcaccacaa acatctatag ttccactatc
    22621 tggttcaagt aacccaagta ccattttcat tgttgtagtc ttacctgcac cattcttacc
    22681 tacaaaccca aagactgacc cttctggtac agtaaaattt agattatcta ttatctttcg
    22741 attaccaaaa ccttttgata atccatttat tgagacaata ttcatcattc ttcacctctt
    22801 cctattgtaa aatacaaaat tggtcctatt agactaataa acagtactac aataatccat
    22861 attgccttat ttccaatttt ataatttgga tgtctcaata catgaacaag tgcagtaatt
    22921 ataagtatta aatctaaaat aataacagga accaaaagtg gtaagtattc catcaaattt
    22981 tccataatct tattcctcca ataaagataa tttttctata agaaatttgt attctttgtt
    23041 ttcacttaat acagaaaaca ttggattgtt tttaatagca tcaacaatac ctgcttttac
    23101 gattgtatca tcacgtgtaa tacctgttcc aatttcagaa atccatctgt caagtttatt
    23161 aaaaaagtca tcaccatgta ttataattgg gtatatatca cgtgttgcta aatctacata
    23221 ttttcgtaat gatttaatag ctccttcatt atccccatgt attaaattaa tttgtacctg
    23281 tgttaaatat gcagaaagca taatagcagg agataatgta tcaagctcaa atatatctga
    23341 aattgctaga atacgtcttt ctatttcctt catttgtaat ttatctgcat tttgcaaaat
    23401 acccactaga ttttggacta atgatataag gttctgatat gccccaagtt gaaaggtttc
    23461 tcttgcttca tcaatttgac cattcatcat ttgcccctgt gcaagaagta tagattcatt
    23521 tataatggga aaattgctgt tttgcaataa atcaatcact tgtatgggtt gatttaacaa
    23581 tatataacaa gttgcctcca tactttttgc ttgacggcaa atatctatgt cattacaagt
    23641 ttcctgtata cgaataaata tttctaatgc ttcactaatt aatagttcac gctttttttc
    23701 atctacaata tcatagtgat taactattaa aagacccatc cgaaaaatca aaggaaaaca
    23761 tgaataatat ttttttatga tttctcttat ttcatccatg acttcatcaa atgatttaac
    23821 cgtaaagtct ttgcacagtt tattatacag tttattaata tcttctttca tcatttgagg
    23881 ttcataacat attaactcat caacggtaat gttgaaatat gtcgcgagtt gtggtaaaaa
    23941 ggttatgtca ggatagcttt gtccagtttc ccatttagat acagaagctt tagaaacgcc
    24001 tatataattt gcaagttcat cttgtgtaat tcctttttct ttacgcttta aaatcagcgt
    24061 ttttgcaatg tttaattcat tcatgtcaac ccctccttat gtataattat atccttaaac
    24121 atgtaaagct aacaagcgat ttgaagttga cttttgacga aaaaatcaac cacagagaaa
    24181 caaataaaca atcatataaa atatatattt aaatatattt taaattt
//
LOCUS       DAEPXF010000047        24174 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain HB27
            SAMN13376389-rid12479423.denovo.049, whole genome shotgun sequence.
ACCESSION   DAEPXF010000047 DAEPXF010000000
VERSION     DAEPXF010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13376389
            Sequence Read Archive: SRR10518432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 24174)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24174)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001069624.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001069624.1
            Genome Coverage       :: 315x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/15/2021 01:54:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,165
            CDSs (total)                      :: 4,076
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 89
            rRNAs                             :: 10, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 10 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 19 of 69
            Pseudo Genes (incomplete)         :: 44 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 10 of 69
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24174
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13376389-rid12479423.denovo.049"
                     /strain="HB27"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="China: Hebei"
                     /collection_date="2017"
     regulatory      248..349
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            422..1765
                     /locus_tag="KRL67_001619"
     CDS             422..1765
                     /locus_tag="KRL67_001619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HBH1522506.1"
                     /translation="MVPNTNNVQPGGNNSSLSFLDKMFKFSERGSSLKTEVIGGVTTF
                     LTMAYIVFVNPAILSEAGMDKAALITVTILATAIGTLIFAILGNAPFALAPGMGLNAF
                     FTYSLVIGAGIPWQTGLGVVFLSGCVFFTLAVTGLRKTIADAIPRELAVASSAGIGLF
                     LAFVGLKNMGVIIANPDTIVSLADFTPTVLIALFALILMAILEYKKVKGGILISIVVA
                     TIISVIFGFVELPTAIISTPPSIAPVAFKLDIVGALKFSLIGPIFSFMFIDMFDSLAF
                     LITCCKQMGLEDENGEIKDLGRMLYADVSSTLIGSFLGTSTVTTFGESASGIAAGART
                     GLASIVTAALFLATLIFTPLLGIVPTYAAAPALVMVGVFMFESIKQIDFADSKIAVPA
                     FVTVLLMPLTYSISIGLCFGFISYIIMHVVAKETDKISITLWIIGLLAVVNLVLS"
     gene            1943..3307
                     /locus_tag="KRL67_001620"
     CDS             1943..3307
                     /locus_tag="KRL67_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HBH1522507.1"
                     /translation="MSQLLIKNAYLITMNADREVYENGSILIENNIIKEVGKFDINIV
                     DKDAEIYDAKGKILMPGLVNTHVHLSQQLGRGLADDVVLLTWLRERIWPYESSFNYED
                     SLISSTACCVELIKSGVTTFLESGGQYVDAMVEAVDKTGLRACLAKSVMDTGDGLPEA
                     WQKTTDEEINTQLDLFHKFNNTSNERIKIWFALRTIFNTTEELLVRTKELADKYNTGI
                     HMHIAEIADEISFVKQNNGVGTVEYLDKLGVLGPNLLAAHTVWLTHKEIDLFRLHDVK
                     VSHDPGSAMKVVLGFASIPEMLEKGISVSIGTDGAPSNNRMDLMRDMYLTSLIHKGRT
                     LNPTVVPAEEVLEMATINGAKCALLEKEIGSLEVGKKADMIILNPDTIHCLPMHNPIG
                     NIVYSMTSENVDSTICDGKWLMKERKVLVVDESELLNKVKEQASKIKDKAEVKIPSKF
                     KIIK"
     gene            complement(3482..5347)
                     /locus_tag="KRL67_001621"
     CDS             complement(3482..5347)
                     /locus_tag="KRL67_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIRAN domain-containing protein"
                     /protein_id="HBH1522508.1"
                     /translation="MENVFKRLQEFNGYDGYKESFEMNYLCIYESIPLREQVELANNL
                     VDEILNMYKSESNEIYLLEDSNSKSLICYFEIFMKKINTLVKEMIIDEKWLYKLTKEL
                     IYKSKKVEYVKLGLILSEKYLNVENLREVVDTFSKSGEYVFYLSNTIKKLEFYNTYLF
                     NLSKKATGSIKVFAIVNMENLDSKINSYLIEYGYKDAKYERLLMNYIISIVDLNEYLE
                     KRDLDKEKINNLARLICNYLLSVEFKYIGNKLELVNRFLPTVVNYGTNFESLYSIFLI
                     AINVLKDENIECNKVEFEKEINDILLSEKWKNMYFEALRDASGKTEDMIKMSEIYDVN
                     LSFDDLLPYLNRDIRDFEVYWHISKKGTTSSRLKLLNFFEERFKVDDLIGKMKDIEKD
                     KLTQEYYDDMLFFIVLKGSKSLYPEGKNISLKGIFGNINEVRKESINILKRYREKLSL
                     EELKMVKEAYEKEKNVILKDELRRVLYESNNLKKEFVNIEKIKVDEHGKDIYLTSIAV
                     AGSRFRNREYLEKELEKSKIYYLTREKDNLYDEKAIKIVGETGYVIGYVPRKENYILS
                     NLLDGGKLLYCRVTEYNLYEDCIYANVYLSYKDVIETVENSLKMVLDKSKIKLIN"
     gene            complement(5592..6800)
                     /locus_tag="KRL67_001622"
     CDS             complement(5592..6800)
                     /locus_tag="KRL67_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004455016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBH1522509.1"
                     /translation="MNNKKYYPTAIALYFSYFLLGIGISILGQYKPEFSSMWGAKTLS
                     DGTLDVSIVLAVIAALGLGRLISYPFAGPISDKYGRKVSGLIGNFLHAIFFVGIVFSP
                     NFYIAYVFAIIGGAANSFLDTCVTPSCMEIFASLGTIANMFTKFTIALAQFLLPFIIG
                     FVAANSISFKVIFIVTAILIVVDAILIAVLPFPPANNVIDNKGKTVKSEKMKFTPTSI
                     ALVCIGFTCTSTFVLWLNCNQELGKLYGMADPTKIQSFYSMGVICAVLITSLLIKKYI
                     KPIRILVIYPIIALLMLLVVYFVQTPTICMLGGFVIGYSAAGGVLQLTTSTANEMFPT
                     NKGKITSIVMIASSIANYVILNIAGIITKSGGVNGPKYVVLFNVAITFVGILLALFVN
                     MRYGKEKVYD"
     gene            complement(7000..7863)
                     /gene="aroE"
                     /locus_tag="KRL67_001623"
     CDS             complement(7000..7863)
                     /gene="aroE"
                     /locus_tag="KRL67_001623"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004455017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HBH1522510.1"
                     /translation="MEISGRTGLFALIGTPVGHSKSPVMYNYSFKKLDLDYRYLAFDI
                     TVDKVKEALLAIKTFNIKGANVTMPCKSAVTEYMDELSPAARIIGACNTIVNDNGKLV
                     GHITDGVGYVRNLKENGVEVKGKKITIMGAGGAATAIQVQCALDGAREISIFNTKDDF
                     YKRAEQTVENIKKDVPECVVNLYDLEDTNKLYEEIESSDILTNATLIGMKPYDNETNI
                     KDTSVLRKDLVVTDVVYNPKKTKMIEDAEANGCKAIGGLGMLLYQGAEAFNLYTGLEM
                     PVEEVNELCFK"
     gene            complement(7945..9867)
                     /locus_tag="KRL67_001624"
     CDS             complement(7945..9867)
                     /locus_tag="KRL67_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HBH1522511.1"
                     /translation="MKISSMFTPIRIGSMTVPNRFVVPPMGNNFANTDGTLSETSKAY
                     YLERALGGFGLITIESSVVDKKAKGGPRKPCLYDDSTIDSFKNVIDACHDAGSKVSIQ
                     LQHAGSEGNEKVAGHPLKAASAIPASNGRNTPLAITTEEIYELIESYGDAALRAQKAG
                     ADAVEVHCAHGYLVSSFISQRTNKRVDEFGGCFENRMRLPRLIIENIRKKVGNSLAIL
                     CRINSTDDVLGGIDVHDSSVIAAYLEDCGIDGLHVSRSIHIHDEYMWAPTTLHAGFSA
                     ELVTEIKKAVSIPVITVGRYTEPQFAELMVRQGRCDLVAFGRQSLADPEMPNKAKNGK
                     LDEMIPCIACLQGCVPNMFQGKPIACLANPVLGHEAELKSAEISKEVLVVGGGVGGML
                     AAWICAKRGHNVTLVEKSEVLGGQMRLAAYPPGKGDITNLVRSYISKCNQYGVKICTN
                     TEATVELIKEKSPDAVIIATGATPLVLPIPGINDSGLIHAVDLLDGKKSCGKKVLVVG
                     GGMVGCEVAAFLGEQEHEVTVIELREEVGADVISEHRKFLMNDFENYNIHTITGAKVS
                     KFYDNGVDYLLTDGTEHDLKGFDSVVLAMGSRNYDPLSEVIKEVVKETYIVGDAVKAR
                     RALDATKEAFEVALNI"
     gene            complement(9946..10794)
                     /locus_tag="KRL67_001625"
     CDS             complement(9946..10794)
                     /locus_tag="KRL67_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HBH1522512.1"
                     /translation="MNNQKNLNIPITISSYTLGTDVSFRDRVRIAKEAGFDGIGLRAE
                     NYIDAKNAGVTDEEMIAILNEYDMKVTEVEYITQWGTEEDRTEAQREKEQNVYHMAHL
                     FNVKHINCGLLEMIPEEQIITALGELCDRAGELIIGLEFMPYSGVPDLATAWRVVKGC
                     NRENAMLILDTWHWARAKQTAEDLAPVPADKIVSIQVCDVLETPYEKLRDESLHDRLA
                     PGEGYGNTEEFVKIIKEHNISPRVIGAEVISDSLISKGLSTAAETVFNATKKVLDKAW
                     SEVSPK"
     gene            11208..12089
                     /locus_tag="KRL67_001626"
     CDS             11208..12089
                     /locus_tag="KRL67_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBH1522513.1"
                     /translation="MNLYHLRYFVTLAHLEHYTKAAENLSITQPSLSHAISLLENELG
                     VALFEKEGRNIVLTKYGKIFLKDVEKSLEILDSSVKSLKITGTGEGQIDLAFLRTLGT
                     DFIPDIVHKFLKSNPAKSIDFKFHTGVTTDIIQGLKERKYDIAFCSKLEKEKGIEFIP
                     VAKQDLVLIVPYSHPLAANDTIDLKETIPYPQIVFNQRSGLRYIVDDMFKKINQQPNI
                     VYEVEEDQVIAGLVAKNFGIAVVPNMNMLSFTKVKVIQIIHPSWERNFYLAFIKDRYL
                     PPAIKNFKNFVIKNAQL"
     gene            complement(12297..13160)
                     /locus_tag="KRL67_001627"
     CDS             complement(12297..13160)
                     /locus_tag="KRL67_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004455022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3800 domain-containing protein"
                     /protein_id="HBH1522514.1"
                     /translation="MLSSYVKRVSKIIKKKYIMFIDESGRADINHEDPFTLTGVIFEY
                     KYAVSQGDYVCSLRKEMDAFKEYCFGTSNIGIHLTDISRKSRAFEKFDDSQIKLFYDE
                     LPLFLSRLECTIISVTVDKARLKKYYAPSKEPYVVAFIHVLQNFYSFVNNETVESARI
                     VIESRDDVSNLKVQKAFFDVFNNGTIYLDIDEELRDKVKGFIIAKKEDVDYREGLEIA
                     DILCNPLSRARRGLIEANPKCMQYGEKNKIFKSVRNKIYTPTSVNDIRNWGFKEVPIV
                     EPVGPWLEKMR"
     gene            complement(13315..15249)
                     /locus_tag="KRL67_001628"
     CDS             complement(13315..15249)
                     /locus_tag="KRL67_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp22"
                     /protein_id="HBH1522515.1"
                     /translation="MKKSLKKYLVLALTLVLVFACGVPESSAASKHVIIVNSRKNTLG
                     YFVNNKLVKEFRVATGKKGSETPTGKTKVVNKIKNRPYYKGNIPGGSPRNPLGDRWMG
                     LALKGTYGDTYGIHGNNNESSIGKHISGGCIRMHNKEVRWLFDQVPVGSDVIIDYSND
                     SYVKIAAKYKINLNQTGWKTENGKKYYVKSDGTYQKNSWLKVNGKMYYFDASGVMQTG
                     WKTINNKKYYLGTDGTRVSGWKVIDGKTYYFNSDGVMQTGWQEKNGKKYYLGSDGLSV
                     TGWQEIDGNKYYFDKTGIMQTGWQQIDGKSYYLDKDGKMLTGSQKIDGKDYTFNEDGT
                     INPTWDTIIGANRFDTAKKISSVGNWNADSSDTVILVNGNAIADGITATPLASSYDST
                     ILLTNTDNLPTETVEEMKLLAPKTVILIGGENAISSKLEQEIKTTFNTETKRIAGQDR
                     YQTATRIAEELGNREEIKTAYMVSGNGEADALSVASKAGEEKQPIILVNKDGITEESY
                     KWLTERKLENAYFIGGPSAINDSVIAKMNDITTEDISGNRIYGDSRVDTNAKVIEKFY
                     GDTDLQAILVSKSDALVDALSAGPLAVKLHSPIVLMDNSGLSSEQQRVFANKKVETPY
                     QIGGGVSYIVMDKLMDILAK"
     gene            complement(15361..16374)
                     /gene="galE"
                     /locus_tag="KRL67_001629"
     CDS             complement(15361..16374)
                     /gene="galE"
                     /locus_tag="KRL67_001629"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HBH1522516.1"
                     /translation="MAVLVAGGAGYIGSHTAIELLESGYEVVIVDNLSNSNSIVVDRI
                     KELSKKPVKFYNIDIRNKDEMHVVFKENNIESIIHFAALKAVGESVEKPIEYYSNNLI
                     STLNLFELMREYGVKKFVFSSSATVYGDPHTCPILEDFPLSVTNPYGRTKLMIEQMLV
                     DISKADKSLDIALLRYFNPVGAHKSGRIGEEPNGVPSNLMPYITKIAVGKLKELSVYG
                     NDYPTHDGTGVRDYIHVLDLAAGHVKALQKLEENPGLVVYNLGTGKGYSVLDLVKAFS
                     KASGKEIPYKIVGRRAGDVAMCYADSSKAEKELGWKAKYELEEMCEDSWRWQSMNPNG
                     YEE"
     gene            complement(16398..17267)
                     /gene="galU"
                     /locus_tag="KRL67_001630"
     CDS             complement(16398..17267)
                     /gene="galU"
                     /locus_tag="KRL67_001630"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HBH1522517.1"
                     /translation="MTVKKAIIPAAGLGTRFLPATKSQPKEMLPIVDKPTLQYIIEEA
                     IESGIEEILIITGRNKKSIEDHFDKSVELELELEQKGKTEMLEMVRDISNMVNIHYIR
                     QKEPKGLGHAIYCAKSFIGDEPFAVLLGDDIVDSDVPCLKQLIDTYNEYKTTVLGVQK
                     IAKEDTNKYGILDVKHIEDRVYKVKDMVEKPAIEEAPSDIAILGRYIITPAIFSILEK
                     QTPGKGGEIQLTDALQTLGKQEAIYAYNFEGRRYDVGDKIGFLEATIDFALKRENLKD
                     DLMDYMRKKVSEK"
     gene            complement(17465..17785)
                     /locus_tag="KRL67_001631"
     CDS             complement(17465..17785)
                     /locus_tag="KRL67_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH1522518.1"
                     /translation="MSSKYNVWSFNYEFLGLNSGDEKNNEVYYEIDLDEILNENYIDE
                     DIDSDLDSDGNSILGELISEMDIDEDVSVDMTYQELEVDLEDLDSYLDEDIDSDIKGI
                     LDEI"
     gene            complement(17775..19766)
                     /locus_tag="KRL67_001632"
     CDS             complement(17775..19766)
                     /locus_tag="KRL67_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH1522519.1"
                     /translation="MLKLGEKIIYELSDGFELSGISRYSKEYKKLHITNLGNIIISKS
                     DEVNKDTGVKYIYSFSDNKEKLEAVLEDSQIIVYGNNLPFYIKSENSSKNVTANLTMK
                     ITLDEYDLICKSQREFIFYLKDNMTILSLDDNKFYMGGINKDNEKFIFISGKNRFEIN
                     FDDIERYILEDKRVSLKGYFHMEREGIIVRSVSIFNNNIDSVVPSDLNERVKDNQKIG
                     NLPKDCDIVFCKISGNIDGFDYKNTNMLLVKYQDQLIFINKKSKKTIVKSAKDNCSKL
                     NLGEDIIIYDNKNVFNLHINDKNREIMQIDDLKDIENEIVGYTLKHAPFFIQEDFDSL
                     TILKSFQKEIISIKNSDIKDIVINKELENENSNFVETEIKFNNQKILLNLSKNMVQKL
                     MQDVFIYAKQPLLKENSIEVIYKNWSKAMNDMIIFNFFGNIYYMKSEFDKILEKELND
                     EIRIEVINSLYKQIQEQRNNLDLLSAYMPRILENQEIDLFEKYNTKLDVQVFKQIKNL
                     LSDLSYNISSYLNEVEKSLDNIIFVISGEDKKKYNYRMLKESESASLDVFLKQAISRL
                     NHLVENMYPYYVDETSREMFKLFELLWKNYRNIDDDSIKEILFERITNTYVFKQLTLN
                     NSTKERRKDIIEKIYNSVDYGTNKLDENMFFTGGIKYVK"
     gene            complement(19889..22450)
                     /locus_tag="KRL67_001633"
     CDS             complement(19889..22450)
                     /locus_tag="KRL67_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HBH1522520.1"
                     /translation="MKVLSENNIIRNTVLNVTASFLKQESKINEKLDGVLEKKFEKVE
                     FNEAKYAELLKFNILFYKTLARNTEPLIGKWIVDKYIPEIDELEKDLELTTAKCRKYV
                     NKAMKDGLDSLKANDLNSFLAYDKMELSERRRRLEKDYKVLNLYKDLLNISLRKISLE
                     KKECGDLFLKNQADAKRELKREIIFCVNKILASNKDVVPNNVEENNEQDRVEIKAAEI
                     EDLQLGKDNSENDMLLNEIKNSKVKHESKSNDIESKFIDKLNSLNNESISQTIHNEYK
                     KLCGLEELHSVEGYGFGKEIIKDFACATVVLEFLKRRNRDLIEGAMRLTIIGEFGPEN
                     FKEFMDYVIKNKTEISEDTWNKGHSLIKDNYSELENHEIASKRTRRNKDIDIEEYIYM
                     IKNADKDICFRSSISIEDDAEEETKEEVDSNSQDIGDVVEDKDITDKEYDSNKEDIIE
                     PENKKSKKKAKLFGFIKKDNEEVEQEEENLNDISPDIILDKPVENHQVKSEEIEQNEL
                     KEIKQEETSQHIEEERSVKIEKPINNNLDEKVSSNNESKLEKESKNLEDKKAKETKEE
                     KEIKEEKLENEKSVVIPIKKKENSNKKSKNSSKDKYIENKKEINNYISDKEDSLDDEE
                     VVSKKSRLKETIIAVVIVAIVGVGYFITVGNNKKNDKEDIPKSSTQQQANNKLTEEEK
                     KAQAEKEKKEAEEKAKAEKEAQEKAQAEKKAKEMEAYKDGKGVYYTVYAGSLKVEKTA
                     KETAKEYEAKGISSTIIQENGYYKIKIGDYSQYGEAQEKCNELAKKSIDTYIAMYDKY
                     YDYKLEELKESAPSLSAEELKQKYEDLRSELKNKSGYREYVKHLDKLYEEIVEGA"
     gene            complement(22728..23062)
                     /gene="rnpB"
                     /locus_tag="KRL67_001634"
     ncRNA           complement(22728..23062)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="KRL67_001634"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            23194..23871
                     /gene="srtB"
                     /locus_tag="KRL67_001635"
     CDS             23194..23871
                     /gene="srtB"
                     /locus_tag="KRL67_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sortase SrtB"
                     /protein_id="HBH1522521.1"
                     /translation="MKKLYRIIINIILVLVILYSGFNIYSKLTKYNHDTKISSELQKK
                     EYKKEDLSKINSDFKFWLSVENTNINYPVVQSKDNSYYLDKDFYKKDSISGTLFMDYR
                     NKSIDDKNIIIYGHNMKNKTMFNNLNKFKDADFFKKNNKIKITLNGKEFLYDVFSAYI
                     VESDYDYLKTNFNNESDYQNYINDITSKSLYKSPVKVNSNDKIVTLSTCTYEFDDARM
                     VIHGRLV"
     gene            complement(24022..24096)
                     /locus_tag="KRL67_001636"
     tRNA            complement(24022..24096)
                     /locus_tag="KRL67_001636"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(24062..24064),aa:Thr,seq:tgt)
BASE COUNT         7725 a         4163 c         2715 g         9571 t
ORIGIN      
        1 aaaagttttt acgaaatatc catgtattca gtacttttat aattatatca caaactcatt
       61 tataataaac ttccattaat agtataaaac aagttagaat aattgacatt atcctcatac
      121 atattcaatt attggaaaaa gtaatttttg tcaataaata accttaattt tttataaaat
      181 cgacaaaaaa acgaataatt ttattatatt tattgtaaat aatacaatat tacgatataa
      241 tacaacagaa aaacaaatag aaaatgttca tataatatta gtaatacggt ctaatagttt
      301 ctacaaactc acctcaaatg ggttttctat gaatagaata tcaactttga atatatgttc
      361 tattctatct atatgaatat atattttttt gtttatatct taaagttttt tggaggtaaa
      421 aatggtacca aacactaaca atgtacaacc aggtggaaat aattcatcat taagcttttt
      481 agacaaaatg tttaaattta gcgaacgtgg ctctagcctt aaaacagagg taataggtgg
      541 agttactaca ttcttaacaa tggcatacat agttttcgta aatccagcaa tattatctga
      601 ggctggaatg gataaagccg cccttataac agttaccata ctagctacag ccattggtac
      661 acttatattt gcgattttag gaaatgcacc atttgctcta gctcctggta tgggattaaa
      721 tgcatttttc acatattctt tggttatagg ggcaggaatc ccatggcaaa caggtcttgg
      781 ggtagttttt ctatctggat gtgtcttctt tacactcgca gtaacaggtc ttcgaaagac
      841 aattgcagac gcaattccta gagaattggc agtagcatct tcagcaggta taggtctttt
      901 ccttgctttt gttggtttaa aaaatatggg tgttataata gctaatccag atactatagt
      961 ttcacttgca gattttacac caacagtttt aatagctctt tttgctctta tcttaatggc
     1021 aatactagaa tataaaaaag ttaaaggtgg aatattaata agtatagtag ttgcaacaat
     1081 aatatctgtt atatttggat ttgtggagtt gccaacagca ataatctcaa cacctcctag
     1141 tatagcacca gttgctttta aattagatat tgtaggtgca ttgaaattct cattaattgg
     1201 acctatattc tctttcatgt ttatagacat gtttgattca ttagcatttc ttattacttg
     1261 ttgtaaacaa atgggtcttg aagatgagaa tggtgaaata aaagatttag gaagaatgtt
     1321 atatgcagat gtttcttcta ctttaatagg ttcattttta ggaactagta ctgttacaac
     1381 atttggagaa tctgcttctg gtattgcagc aggtgcaaga acaggacttg cttcaatagt
     1441 aacagcagca ttattcttag ctacacttat atttacacca ttacttggta tagttcctac
     1501 ttacgctgca gctcctgcac ttgtaatggt tggtgtgttc atgtttgaaa gcataaaaca
     1561 gattgatttt gctgatagca aaatagcagt acctgctttt gttacagttt tattgatgcc
     1621 tttaacttat agtataagta ttggtttatg ctttggattt atttcatata taataatgca
     1681 tgtggtagct aaagaaactg ataaaatcag tattacactt tggattatag gtttacttgc
     1741 agttgtaaac ttagttctaa gttaatattt taaataattc taggtcaata ttttaaatta
     1801 tatagaataa aatcttattt attttaaatt tataaatagc aaaatcactt tatatgtaaa
     1861 ttgattttgc tattaacttt gtatagaaaa tttattatca taatacatag atataaaaat
     1921 atatagaaaa ggagtggtgt ttatgtcaca attacttata aaaaatgcat atctaattac
     1981 tatgaatgca gatagagagg tatatgaaaa tggaagcata cttattgaaa ataatattat
     2041 aaaagaggtt ggtaagtttg atattaatat tgtagataaa gatgcagaga tatatgatgc
     2101 taaaggaaaa atactaatgc cagggctagt caatacacat gttcacttat ctcaacaatt
     2161 aggacgtggt cttgcagatg atgttgtact tttaacttgg cttagagaaa gaatttggcc
     2221 atacgaaagt agcttcaatt atgaagattc cttgatttca tctactgcat gttgtgttga
     2281 attgataaaa tctggtgtca ctacattttt agaatctggt ggtcaatatg tagatgctat
     2341 ggttgaagct gttgataaaa ctggacttag agcatgttta gctaaatcag ttatggatac
     2401 aggagacggt cttcctgagg catggcaaaa aactactgat gaagaaatca acactcaatt
     2461 agacttattc cataagttta ataacacatc taatgagaga attaaaatat ggtttgcatt
     2521 aagaactatt tttaatacaa ctgaagaatt acttgttaga actaaagagt tagctgataa
     2581 atacaataca ggcatacata tgcatattgc tgaaatagct gatgaaattt catttgtaaa
     2641 gcaaaacaat ggagttggaa cagttgaata tcttgataaa ctaggtgtat taggaccaaa
     2701 tcttttagct gctcacacag tttggcttac tcataaggaa attgatttat ttagattaca
     2761 tgatgtaaaa gtttctcatg acccaggttc agctatgaaa gtagttctag gatttgcatc
     2821 aattcctgaa atgttagaaa aaggaatatc tgtttcaatt ggtactgatg gagctccttc
     2881 aaataataga atggacctta tgagggatat gtatttgact tcattaatac ataaaggtag
     2941 aactttaaat ccaacagttg taccagctga agaagtgtta gaaatggcta ctataaatgg
     3001 agcaaaatgt gctttattag aaaaagaaat tggtagtctt gaagtaggaa aaaaagctga
     3061 tatgattatt ctaaatccag atacaattca ttgtttgcca atgcataatc caattggaaa
     3121 tatagtttat tctatgacta gtgaaaatgt agattctact atttgtgatg gtaaatggtt
     3181 gatgaaggaa agaaaagtat tagttgtaga tgaatctgaa ttgttaaata aagttaagga
     3241 acaagcttct aaaataaaag ataaggcaga agttaaaatt ccatctaaat ttaaaatcat
     3301 aaaataatat aaatttcaat tgttttgtaa atatgaatat ataaatatat ttatgcatca
     3361 tatatacaaa acaattgttt ttttattttc tattcattac cattgctcat aaaatacatc
     3421 ataattccag aaaaataacc aatctgttag acttatatca tctaagtaat ctataaattt
     3481 tttagtttat aagttttatt ttactcttat ctaaaaccat ctttagagag ttttcaaccg
     3541 tttcaattac atctttatag ctcaagtata catttgcata aatacaatct tcatataaat
     3601 tatattcagt tactctacaa tacaagagtt ttcctccatc taataaattg ctcaaaatat
     3661 agttttcttt tcttggaaca taacctatta catatccagt ttcaccaact attttaatag
     3721 ctttttcatc atatagatta tctttctctc tagttagata gtatatctta ctcttctcca
     3781 attctttttc aagatattct ctatttctaa accttgaacc agcaacggct attgaagtca
     3841 aatagatatc cttaccatgt tcatctactt ttattttttc aatatttaca aattcttttt
     3901 ttaaattgtt actttcatat aatactcttc ttaattcatc cttcaatata acattttttt
     3961 ctttttcata ggcttcttta accattttta attcttcaag agataatttc tctctatatc
     4021 ttttcaaaat atttatggat tcttttctta cttcatttat atttccaaat attcctttta
     4081 aagatatatt ctttccttca ggatatagtg atttacttcc tttaagcaca ataaaaaata
     4141 acatatcatc ataatattct tgagtcaatt tatctttttc tatatctttc atttttccaa
     4201 ttaaatcatc tactttaaac ctctcttcaa aaaaatttaa caattttaat ctggaacttg
     4261 tcgttccttt tttagatata tgccaatata cctcaaaatc tctaatatct ctattcaaat
     4321 acggtaacaa gtcatcaaaa gataagttta catcataaat ttctgacatt tttatcatat
     4381 cttctgtttt tccactagca tctcttaatg cttcaaaata catatttttc catttttcac
     4441 taagcaagat atcatttatt tctttttcaa attcaacctt attgcactct atattttcat
     4501 cttttaaaac atttatagct attaggaaaa ttgaatataa actttcaaag ttagtcccat
     4561 aatttactac agtaggtaaa aatcgattca ctaattctaa tttatttcct atatatttaa
     4621 attctacact tagcaagtaa ttgcatataa gacgtgctaa attatttatc ttttctttat
     4681 ctaaatctcg tttttcaaga tattcattca aatctacaat tgaaataata taattcatca
     4741 ataatcgttc gtacttcgca tctttataac catattcaat tagataggaa tttattttag
     4801 aatctaaatt ttccatatta acaatagcaa atacttttat tgaacctgta gcctttttag
     4861 ataaattaaa taaatatgta ttgtaaaatt caagtttttt aatagtatta ctcagataaa
     4921 atacgtattc tccagattta gaaaatgtat ctacaacttc tcttaagttt tctacattta
     4981 gatatttctc acttaaaatt aaccctagtt ttacatactc aactttttta ctcttatata
     5041 ttaattcttt agttaattta tacaaccatt tctcatcaat aatcatttct tttaccaaag
     5101 tatttatctt tttcataaaa atttcgaaat aacaaattaa agacttagaa ttactatcct
     5161 ccaataaata aatttcatta gattcacttt tatacatatt taaaatttca tctactagat
     5221 tatttgcaag ctctacttgc tctcttaaag gtatactttc atatatacat aggtaattca
     5281 tttcaaatga ttctttgtat ccatcataac cattaaattc ctgtaatcgc ttaaaaacat
     5341 tctccaccaa attatcctcc tttacatgat gtaatttata ttgattatac tacttatttc
     5401 gtcaagttta catttatacg caaaaaaata ttactagaaa ttgaaattct agtaatatct
     5461 gtattattaa ttatgtaaat accctatctt ataactgtaa ataatatcct tattaatttt
     5521 aattattttt aatagaaacc catcctaaat ttggacttct ctataaatat attatactaa
     5581 tattagacat cctaatcata cactttttct tttccatatc tcatgtttac aaacaatgca
     5641 agtaaaattc caacaaatgt tattgctaca ttaaacaata caacatattt tggtccatta
     5701 actccccctg attttgtaat aatacctgct atatttaaaa taacatagtt tgctatacta
     5761 gatgcaatca taactataga tgttattttt cctttattag ttggaaacat ttcatttgca
     5821 gttgacgttg ttaactgaag aacacctcct gctgctgaat aacctattac aaatccccct
     5881 aacatacaaa tagttggagt ttgaacaaaa tatacaacta atagcataag taaagctatt
     5941 ataggatata taactaatat tcttattggt ttaatatact ttttaataag taaagaagtt
     6001 attaatactg cacatattac tcccattgag taaaaggatt gaatttttgt agggtcagcc
     6061 atcccatata atttacccaa ttcctgatta caatttaacc ataatacaaa tgtagatgta
     6121 catgtaaatc caatacaaac aagagctata cttgttggtg taaatttcat cttctctgat
     6181 ttaactgttt tgcctttgtt atcaataaca ttattagctg gtggaaatgg taacacagct
     6241 atcaaaatgg catctactac aataagaatc gctgttacta taaaaataac tttaaatgat
     6301 attgaatttg ctgcaacaaa tccaattata aagggaagta aaaattgtgc taaagctatt
     6361 gtaaatttag taaacatatt tgctattgtt cctaaactag caaatatctc catacaagaa
     6421 ggagttacac aggtatctaa gaatgaattt gctgcccctc caattattgc aaatacatat
     6481 gctatataaa aatttggtga aaatacaatt ccaacaaaga aaattgcatg tagaaaattt
     6541 cctataagac cacttacttt tcttccatac ttatctgaaa taggtcctgc aaatggatac
     6601 gatataagtc tccctaatcc tagtgcagca ataacagcta aaacaatact tacatccaat
     6661 gttccatctg atagtgtttt agccccccac attgatgaaa attctggttt gtattgtcct
     6721 aaaattgaaa ttccaatacc aagtaaaaaa tatgaaaaat acaacgctat ggcagtagga
     6781 taatattttt tattgttcat aatttttctc ccattcttgt taatttatta acatttttgt
     6841 gtacaataaa ctcatcccca taatggattt aaagtatagt cagttaaagc ttcaaattta
     6901 actggactat gatgcaaaac caactatgac aatttgtaat ttattattaa aaatattgaa
     6961 atacgtatgt tttttcattg tttaagatat cactcttatc tatttaaaac ataattcatt
     7021 aacttcttct acaggcattt ctaatccagt atatagatta aaagcttctg ctccttggta
     7081 taataacatt cctaatccac caatagcctt acatccatta gcttcagcat cttcaatcat
     7141 ttttgttttt ttaggattat aaacgacatc tgttactact aagtcttttc tcaaaacact
     7201 agtatctttt atatttgttt cattgtcata aggcttcata ccaataagag ttgcatttgt
     7261 caatatatca cttgattcta tctcctcata aagtttgttt gtatcttcta aatcatatag
     7321 attaacaaca cattctggta catctttttt tatattctca actgtttgct ctgctctttt
     7381 ataaaaatca tccttagtat taaagataga tatttctctt gctccatcta aagcacattg
     7441 tacttgtata gcagttgctg caccaccagc acccataatt gtaatctttt tacctttaac
     7501 ttctacccca ttttctttta aattacgaac atagcctact ccatctgtta tatgaccaac
     7561 taactttcca ttgtcattta caattgtatt acatgctcca attatacgag ctgctggaga
     7621 aagttcatcc atatattctg taacagcaga tttacatggc atagtaacat tggctccttt
     7681 tatattaaaa gtttttattg ctaaaagtgc ctcttttacc ttatctactg tgatatcaaa
     7741 ggctaaatat ctataatcta aatctaattt cttaaaacta taattataca tcactggtga
     7801 tttagagtga ccaactggtg tacctattaa tgcaaataat cctgttctac ctgaaatttc
     7861 cataactata atctcctccc aatattatca ttttctttgc aaataaccta ttcagttatt
     7921 tacaaagaaa aatttattta ttatttatat atttaatgct acttcaaaag cttcttttgt
     7981 tgcatctaat gccctacgag cttttactgc atcaccaaca atataagttt cttttacaac
     8041 ttcttttata acttcactta atgggtcgta attacgagaa cccatagcaa gtactactga
     8101 atcaaaacct tttaaatcat gctctgtgcc atctgtaagt aaatagtcga caccattatc
     8161 ataaaattta gaaacttttg caccagtaat ggtatgtata ttataatttt caaaatcatt
     8221 cataaggaat ttacggtgtt ctgaaattac atctgcacca acttcttctc taagctcaat
     8281 aacagtaacc tcatgttctt gttctccaag gaaggctgcc acttcacatc caaccattcc
     8341 acctccaaca acaagtactt ttttcccaca agatttttta ccatcaagaa ggtctacagc
     8401 atgaattaat cctgaatcat tgattcctgg tataggaagt acaagtggag ttgctccagt
     8461 agctataata actgcatcag gtgatttctc ttttataagt tctactgtag cttctgtatt
     8521 agtacatatt tttacaccat attgattaca ttttgaaata taacttctaa ctaagttagt
     8581 aatatctcct tttcccggag gatatgcagc taaacgcatt tgcccaccaa gaacttcaga
     8641 tttttcaact aaagttacat tatgtcctct tttagcacat atccatgctg caagcatacc
     8701 tccaacgcct ccaccaacaa caagaacttc tttgctaatt tcggctgact taagctctgc
     8761 ttcgtgtcct aatacaggat ttgcaagaca tgctataggt ttgccttgga acatatttgg
     8821 tacacatcct tgtaaacatg caatacatgg tatcatttcg tctagctttc catttttagc
     8881 tttattaggc atttctgggt cagccaaact ttgtctaccg aatgctacta aatcacatct
     8941 tccttgtctg accattaact cagcaaactg tggttctgta taacgtccaa ctgttattac
     9001 tggtatacta acagcctttt ttatttcagt aactaattct gcactgaatc cagcatgtaa
     9061 agtcgtagga gcccacatat attcatcatg tatatgtata ctacgtgaaa catgaagtcc
     9121 atctattcca caatcttcta gatatgctgc tattactgaa ctatcatgta cgtcaattcc
     9181 ccctaggaca tcatcagtgc tgtttatacg acataatatc gctagagaat ttcctacttt
     9241 tttacgtata ttttcaatga taagacgagg taatctcatt ctattttcaa agcatccacc
     9301 aaactcatct acacgcttat ttgttctttg tgatataaaa ctactaacca gatatccatg
     9361 agcacaatga acttcaacag catctgctcc agctttttgt gcacgtaatg cagcatctcc
     9421 ataagattct ataagttcat atatttcttc tgttgttatt gctagtggtg tattacgacc
     9481 atttgatgca ggtattgcag aagcagcttt taatggatgt cctgctactt tttcatttcc
     9541 ttcagaacca gcatgttgta attgtataga taccttcgaa cctgcatcat gacaagcatc
     9601 tattacattt ttaaaactat ctattgtgct atcatcatat aaacaaggct tacgaggacc
     9661 tccttttgct tttttgtcta caacactaga ttctatagtt attaatccaa aaccacctaa
     9721 agcacgctca agataataag ccttagaagt ttcacttaaa gtaccatctg tgtttgcaaa
     9781 attgtttccc ataggaggaa ctacaaaacg atttggaact gtcattgaac caattcttat
     9841 aggtgtaaac atagaactaa tcttcataag gttttccttc ctttcaagtt ttatatttaa
     9901 gagttttata ttataatatt ctgtatattt ttatattata taagtttatt ttggagatac
     9961 ttcagaccat gctttatcca aaactttttt agttgcatta aatacagttt cagcagcagt
    10021 agataaccct ttagatatta gagaatcaga tattacttct gcccctatga ctcttggtga
    10081 tatattgtgt tcttttatta ttttcacaaa ttcctctgtg tttccatatc cttcacctgg
    10141 agccaaacgg tcatgtagtg attcatccct aagtttttca taaggagttt ctaaaacatc
    10201 acacacttga attgatacta tcttatctgc tggaacaggt gcaaggtcct ctgctgtctg
    10261 ctttgctcta gcccagtgcc aagtatctaa tataagcatt gcattttcac gattacatcc
    10321 tttcacaaca cgccatgcag tagccaaatc tggtacacca ctatatggca taaactcaag
    10381 acctataata agttcacctg ctctgtcaca taattctccc aatgcagtta taatttgttc
    10441 ttctggtatc atctccaata atccacaatt tatatgttta acattaaata agtgagccat
    10501 atgataaaca ttttgctctt tttctctttg agcttccgtt ctatcttctt ctgttcccca
    10561 ttgagttata tattctactt ctgtaacttt catatcgtat tcattaagaa ttgcaatcat
    10621 ctcttcatct gtaacacctg cattttttgc atcaatataa ttttcagcac gcaatccaat
    10681 accatcaaat ccagcttctt ttgcaatacg aactctatca cggaatgaaa cgtctgtccc
    10741 caatgtataa gaactaattg taattggaat attaagattc ttttgattat tcatagctta
    10801 aatttgcccc ctttattttc tttataatta atagtatttt tgattttata tttttattta
    10861 tcctactagt agtattattt tgccttaaaa ataaaagtat tttttgattt ctatttatct
    10921 agattttata aatttattgt atcacgagac tttactaata acaaattgat aatttcctat
    10981 taattcatag attttgttta tagattttat ttataagata attcttgtat tcttctatta
    11041 ggtcgtataa atatatttat cttataattc agacaaaaaa cgataaattt ttttaaaagc
    11101 ttttattctt actctataat catatataat aaaaatgtaa aaaatatctt aaaggttaac
    11161 ttctacaaat atataaataa aaatatctat gaaaggatga acaaaaattg aatttatatc
    11221 acttacgcta ttttgttaca ttggcacatt tagaacatta tacaaaagcg gcagaaaatt
    11281 tatctataac acaaccaagc ttaagtcatg ctatttcttt actagaaaat gaacttgggg
    11341 tggctctatt tgaaaaagaa ggtagaaata tagtccttac taagtatgga aaaatatttt
    11401 taaaagatgt ggaaaaatca ttagaaattt tagattcaag tgtaaaatca ttaaaaataa
    11461 ctggtactgg tgaaggacaa atagacttag cttttcttag aacacttggt actgacttta
    11521 tcccagatat agtgcataaa tttttaaaat ctaatcctgc taaatcaata gattttaaat
    11581 ttcatactgg tgtaactact gacattattc aaggtcttaa agaaagaaaa tatgatattg
    11641 cattttgttc gaaacttgaa aaagaaaagg gaattgaatt tatacctgtt gccaaacaag
    11701 atttagtact aattgtacca tatagtcatc cactagctgc aaatgatact atagatttaa
    11761 aagaaaccat accttatcct caaatagtat ttaatcagag aagtggtctt agatatatag
    11821 ttgatgatat gtttaaaaaa ataaatcaac agcctaatat agtctatgaa gtggaagaag
    11881 accaagtaat tgctggatta gttgcaaaaa atttcggaat tgctgtagtc ccaaatatga
    11941 atatgcttag ctttacaaaa gtaaaagtaa ttcagattat tcacccaagt tgggaaagaa
    12001 acttttactt ggcatttata aaagatagat atttgcctcc cgcaattaaa aactttaaaa
    12061 actttgtcat taaaaacgct caattataat ttattaataa aggggttatt tttaaatatc
    12121 ctcttttatt ctttttataa aataaaaaaa tctatacttt taaaagtata gattcctaac
    12181 tgacagtcga catccgagtc tcagtaatta tcgttataca acagcaactg gattcagtcc
    12241 ctgaataact aggttattta tattatatca gattttttaa aacttgtcaa ttgtatctaa
    12301 cgcatttttt caagccatgg tccaacaggc tctacaatag gaacttcttt aaatccccaa
    12361 tttcttatat cattcacact cgttggagta tatattttgt ttctgacaga tttgaatatt
    12421 ttgttttttt ctccatattg catacacttt ggattagctt ctattaaccc tcttcttgct
    12481 ctgctaagag gattgcataa aatatctgct atttctaaac cttctctata gtctacatct
    12541 tcttttttgg caattataaa acccttaacc ttatctctta attcttcatc tatatctaag
    12601 tatattgttc cattattaaa tacatcaaaa aacgcctttt gtacttttaa atttgagaca
    12661 tcatcccttg actctataac tattcgtgca gattctacag tttcattatt tacaaacgaa
    12721 taaaaattct gaagaacatg tataaaagcc acaacatacg gttcctttga aggtgcataa
    12781 tatttcttta atctagcttt atctactgtt actgatatta tagtacactc tagtctagat
    12841 agaaataaag gcaactcatc atagaataac ttaatctgtg aatcatcaaa cttctcaaat
    12901 gctcttgatt ttcttgatat atctgtcaaa tgtattccta tgttactggt tccaaaacaa
    12961 tactctttaa atgcatccat ttctttcctc aaagaacata cataatcgcc ttgacttact
    13021 gcatatttat attcaaaaat tactcctgtc aatgtaaatg ggtcttcatg atttatatca
    13081 gctcttccac tttcatcaat aaacattata tattttttct ttataatctt actcaccctc
    13141 ttaacatatg atgataacat ctatattcat tttatatgag ttttaaaaat attccaagat
    13201 ttttatataa cttaaactta attttatact aaaaaattga gatgtaaaac aatcaagctt
    13261 ttattgattg aatttcacat ctcaattata attatttttc aatatatatt tttactattt
    13321 tgcaagaata tccattaatt tatccattac tatataagat actccgcctc ctatttgata
    13381 aggagtttca acttttttat ttgcaaatac tctttgttgc tctgatgata atcctgaatt
    13441 atccattaaa actattggtg aatgtaattt tacagctaat ggtccagctg ataaagcatc
    13501 tactaaagca tctgatttgc taactaatat agcctgtaaa tcagtatctc cgtagaattt
    13561 ttctattact tttgcattag tatctactct actatctcca tatattctat ttcctgaaat
    13621 atcttcagta gttatatcat tcatttttgc tattactgaa tcgttaattg cacttggtcc
    13681 acctataaag tatgcatttt ctaattttct ttctgttagc catttataag attcttctgt
    13741 aattccatct ttattgacta atattatagg ttgtttttct tccccagctt tagaagctac
    13801 agatagagca tctgcttccc catttccaga aaccatatat gcagttttta tttcttctcg
    13861 atttccaagt tcttcagcta ttcttgtagc agtttgatat ctatcttgac ctgctattct
    13921 ctttgtttct gtattgaaag tagtttttat ttcttgctct aatttagaac ttattgcatt
    13981 ttctccacct atcaagatta ctgttttagg tgctaataac ttcatttctt caacagtttc
    14041 agttggtaaa ttatctgtat tagtaagtag tattgttgag tcatagctac ttgctaaagg
    14101 agttgctgtt ataccatcag ctattgcatt accatttact aatattacag tatctgaact
    14161 atcagcattc cagtttccaa ctgaacttat cttcttagct gtatcaaatc tatttgcacc
    14221 tataattgta tcccatgtag gatttatagt tccatcttca ttaaaagtat aatcttttcc
    14281 atctattttt tgagaaccag ttagcatctt tccatctttg tctaaatagt aagacttacc
    14341 atctatttgt tgccatcctg tctgcattat tcctgttttg tcaaagtaat acttattacc
    14401 atctatttct tgccatcctg ttactgataa accatcagat cctagatagt atttctttcc
    14461 attcttttct tgccatcctg tttgcattac tccatctgag ttaaagtaat aagtttttcc
    14521 atctattact ttccatccag atactcttgt tccgtcagtt cctaaataat attttttgtt
    14581 attaattgtt ttccatccag tttgcattac tcctgaagca tcaaagtaat acatttttcc
    14641 atttactttt aaccaactat tcttttggta tgttccatca gattttacat aatacttctt
    14701 accattttct gttttccatc ctgtttgatt tagatttatc ttatattttg ctgctatctt
    14761 tacatagctg tcattactat aatctataat tacatctgaa cctacaggaa cttggtcaaa
    14821 taaccatcta acctctttat tgtgcattct tatacatcca ccagaaatat gctttcctat
    14881 agaactctca ttattatttc cgtgtattcc atatgtatct ccataagttc cttttaatgc
    14941 taatcccatc catctatctc ctaatggatt tcttggactt cctccaggta tattaccttt
    15001 ataatatggt ctatttttta ttttgttaac tacttttgtt ttccctgttg gtgtttctga
    15061 ccccttttta cctgttgcaa ctctaaattc ttttacaagt ttattattta caaaatatcc
    15121 tagagtattc tttcttgaat ttactataat cacatgttta ctggctgcgc ttgattctgg
    15181 tacgccacat gcaaatacta aaactaaagt cagtgctagt actaaatact tttttagact
    15241 ttttttcact ataacttccc cctcttttag aaacattact acatgtgtct aattataaca
    15301 taaaattacc aataccttat atttacaaat ttgttacatt tatacatata aatatatatt
    15361 ctattcttca tacccattag gattcatact ttgccatctc catgaatctt cacacatctc
    15421 ttcaagttca tacttagctt tccatccaag ctctttttct gcctttgaag aatctgcata
    15481 acacatggct acatctccag cccttcttcc tacaatctta tatggtattt cttttccact
    15541 tgctttagaa aatgctttta ctaaatcaag aacactatac ccctttccag ttcctaaatt
    15601 ataaacaaca agtcctggat tttcttctaa tttttgtaat gcttttacat gaccagcagc
    15661 taaatctaat acatgtatat agtctctaac tccagttcca tcatgtgttg gatagtcatt
    15721 accataaaca ctcaattcct tcaatttacc tactgctatt ttcgttatat aaggcattag
    15781 attactcgga accccatttg gttcttcacc tattctacca cttttatgag caccgactgg
    15841 attaaagtat cttaaaagag ctatatccaa cgacttatct gcttttgata tatctactaa
    15901 catctgctct atcataagtt tagttcttcc atatggattg gttacagaaa gtgggaaatc
    15961 ctctagtata ggacaagtat gtgggtctcc atatacagta gctgaagaac taaatacaaa
    16021 ctttttaaca ccatattctc tcattaattc aaataaattt aaagtactta ttagattatt
    16081 gctgtaatac tcaattggtt tttctactga ttcaccaact gcttttaaag ctgcaaagtg
    16141 tataattgat tcaatattat tttctttaaa tacaacatgc atttcatctt tatttctgat
    16201 atcaatatta taaaatttta ctggtttttt agataattcc ttgattctat caaccactat
    16261 tgagttgcta ttacttaagt tatcaactat aacaacttca taacctgatt ctaaaagttc
    16321 tattgctgta tggcttccta tatatcctgc tcctccagct actaaaactg ccattgtaat
    16381 tacctctttt ctaatattta tttttcactt actttttttc tcatatagtc cattaaatca
    16441 tcttttaaat tttctctctt tagagcaaag tctattgttg cctctaaaaa accaatctta
    16501 tctcctacat catatcttct accttcaaag ttataagcat aaatagcctc ttgttttcca
    16561 agagtttgaa gtgcatctgt cagttgtatc tctccacctt taccaggtgt ctgtttctct
    16621 aatatgctaa atattgcagg agttattata tatcttccaa gtatagctat atcagatgga
    16681 gcttcttcaa ttgcaggttt ttcaaccata tctttaactt tatatactct atcttcgata
    16741 tgttttacat ccaatatacc atacttatta gtatcttctt tagctatctt ttgaacacct
    16801 aatacagttg ttttatactc attataagta tctattaatt gttttaaaca cggaacatct
    16861 gaatctacta tatcatcacc taataatacg gcaaaaggct catcacctat aaaacttttt
    16921 gcacaataaa ttgcatgtcc aagacctttt ggttcttttt gtcttatata atgaatattt
    16981 accatatttg atatgtctct taccatttct aacatctctg tcttaccttt ttgctctagt
    17041 tctaactcta attcaactga tttatcaaaa tgatcttcta tagatttttt atttctacct
    17101 gttattataa gaatttcttc aattcccgat tctatagctt cttctattat atactgtaaa
    17161 gttggtttat ctactatagg aagcatttct ttgggctgtg attttgttgc tggtaaaaat
    17221 ctagtcccta gccctgctgc tggtattatt gcttttttaa ctgtcatctg cattttttca
    17281 cccttcttcg taaattttct attgtaccaa attaatatac catatttata ctttttcttc
    17341 aaactctata ttatcccaat atttaatgct ataagataat ataaggtaga tgaaatttca
    17401 tctaccttta gttttataag ttaatattgt attgtttata ttttatctaa aacttataca
    17461 ggtactaaat ttcatctaag atgcctttta tatcactatc tatatcttca tctagataag
    17521 aatctaaatc ttctaagtca acttctagtt cttgatatgt catatcaaca ctgacatctt
    17581 catctatatc catttctgat attaattctc ctaaaatact atttccatca gaatctaaat
    17641 cactatctat atcttcatct atatagtttt catttaaaat ttcatctaaa tcaatctcgt
    17701 aataaacttc attatttttt tcatcccctg agttcaatcc taggaactca tagttaaaac
    17761 tccaaacatt gtatttactt gacatacttt attcctcctg tgaaaaacat attttcatct
    17821 agtttattgg ttccataatc aacagaatta tatatttttt caattatgtc ttttcttctt
    17881 tcttttgtac tattatttaa tgtcaattgt ttaaatacat atgtatttgt tattctctcg
    17941 aataatattt cttttatact atcatcatca atatttctat agtttttcca caataactca
    18001 aataacttaa acatttccct actagtttca tctacataat aaggatacat attttctact
    18061 aaatggttta atcttgatat agcttgtttt aaaaatacat caagacttgc actttcactt
    18121 tcctttaaca ttctataatt gtatttcttc ttatcttctc ctgaaattac aaagatgata
    18181 ttatctaagc ttttttcaac ttcatttaaa taactgctta tattatatga taaatcagac
    18241 aatagatttt taatctgctt aaacacttgt acatcaagtt tagtattata cttttcaaac
    18301 aaatctattt cttgattttc taatattctt ggcatatatg ctgacaataa atctaaattg
    18361 tttctttgtt cttgtatttg cttataaagt gaatttataa cttctattct tatttcatca
    18421 tttaactctt tttctaaaat cttatcaaat tcagatttca tatagtaaat atttccaaag
    18481 aaattaaata ttatcatatc attcatagct ttagaccaat tcttatatat cacttcaata
    18541 gaattttctt taagtagagg ttgctttgca tatataaata catcttgcat aagtttttga
    18601 accatatttt tacttaagtt taaaagtatt ttttgattat taaattttat ttccgtttct
    18661 acaaaattag aattttcgtt ttcaagctct ttattaatta caatgtcttt tatatcacta
    18721 ttttttatag atatgatttc tttttgaaaa gactttagaa tagtcaagct atcgaaatct
    18781 tcttgaataa aaaatggagc atgttttaat gtatatccca ctatttcatt ttctatatct
    18841 tttaagtcat ctatttgcat aatttctcta tttttgtcat ttatatgtag gttaaataca
    18901 tttttattat catatattat tatatcttct cctaaattca acttagaaca attatctttt
    18961 gctgacttta caatagtctt cttagatttc ttatttataa aaattaattg gtcttgatat
    19021 tttactaaaa gcatgtttgt gtttttataa tcaaatccat ctatatttcc tgatatcttg
    19081 cagaatacta tatcacaatc tttaggaaga tttcctattt tttggttatc tttaactctc
    19141 tcatttaaat cagatgggac tacactatct atattgttat taaatataga tacagacctt
    19201 actataatcc cctctctttc catatgaaag tatcccttca atgacactct tttatcttct
    19261 aaaatgtatc tttctatatc atcaaaattt atttcaaatc tatttttccc actgataaat
    19321 ataaacttct cattatcttt attaattcca cccatataaa acttattgtc atctaaactt
    19381 aatatagtca tattatcttt tagataaaat ataaattctc tttgactttt gcagattaaa
    19441 tcatattcat ccaaagttat tttcatagtt aaatttgctg taacattttt acttgaattt
    19501 tcacttttta tgtaaaaagg caagttattt ccatagacaa ttatttgaga atcttctaaa
    19561 actgcctcta atttttcttt attgtctgaa aaactatata tatattttac acctgtatcc
    19621 ttattaactt catctgattt gctgattatt atattcccta gatttgttat atgaagcttt
    19681 ttatattctt ttgagtatct acttatgcca ctaagctcaa acccatctga aagctcatat
    19741 atgatttttt ctcctaattt caacatacct tcaccaccta aacagaatta taagtatttt
    19801 ataaaggtga acaaaatgtc tcaaaataaa taatattttt tttaatactt tttattctga
    19861 gacatagttt ctcctttttt ataatgctct aagcaccttc aactatttct tcataaagtt
    19921 tatctaaatg tttaacatat tcacgatatc cagatttgtt ctttaactca cttcttaaat
    19981 cttcatattt ttgtttaagt tcttcagcac tcaaagaagg agctgattct tttaattctt
    20041 ctaatttata atcatagtac ttatcataca tagctatata tgtatcaata gatttcttag
    20101 ctagttcatt acatttttct tgtgcttctc catattgaga ataatcacct atttttattt
    20161 tatagtatcc gttttcttga attattgttg aagaaatacc tttagcctca tattcttttg
    20221 ctgtttcttt tgctgttttt tcaaccttaa gtgaacctgc ataaacagtg taatatacac
    20281 ctttaccatc tttatatgct tccatttctt ttgctttctt ttcagcttgt gctttttctt
    20341 gagcctcttt ttcagctttt gccttttctt ctgcttcttt tttctctttt tcagcttgtg
    20401 cttttttctc ttcctcagtt aatttattgt tagcttgttg ctgagttgat gactttggaa
    20461 tatcctcttt atcatttttc ttattgttgc ctactgttat aaaatatcct acaccaacaa
    20521 tcgccactat aactactgct ataatagttt cttttaatct actcttctta gaaacaacct
    20581 cttcatcatc taaagagtct tctttatctg atatataatt atttatttct ttcttatttt
    20641 ctatatattt gtccttagaa gaatttttag attttttatt actattttct ttctttttaa
    20701 taggtattac tactgatttt tcattttcta atttttcttc ttttatttct ttttcttctt
    20761 ttgtttcttt tgctttttta tcttctaagt ttttactttc tttttcaagc ttcgattcat
    20821 tatttgaaga tactttttca tctaagttat tatttattgg tttttctatt tttacactac
    20881 gttcttcttc tatatgttga cttgtttctt cttgttttat ttcttttaat tcattttgct
    20941 ctatttcttc tgattttact tgatgatttt caactggttt atctaaaatt atatcaggag
    21001 aaatatcatt taaattctct tcttcttgtt caacttcttc attgtctttt ttaataaatc
    21061 caaataattt agccttcttt ttactctttt tattttctgg ttcaattata tcttctttat
    21121 tgctgtcgta ctctttatct gtaatatctt tatcttctac aacgtcacct atatcttgac
    21181 tattactatc tacttcttct ttagtctctt cttcagcatc atcttctatt gaaatactac
    21241 ttctaaagca aatatcttta tctgcatttt ttatcatgta aatgtattct tcaatatcga
    21301 tatctttatt tcttctagtt ctttttgatg caatttcatg attttctaat tcactatagt
    21361 tatcttttat tagactatgt cctttattcc atgtatcttc gctaatttct gttttatttt
    21421 taattacata gtccataaat tccttaaaat tttcaggacc aaattctcca attatagtca
    21481 gtctcattgc accttctata aggtctctat ttcttctctt taaaaattct aatacaaccg
    21541 ttgcacaagc aaaatcttta ataatttctt tgccaaaacc atatccttca acagaatgaa
    21601 gttcttctaa tccacataac ttcttatatt cattgtgtat tgtttgagat atactttcat
    21661 tatttaagct atttaactta tctataaact tcgattctat atcatttgat tttgattcat
    21721 gttttacttt tgaattttta atttcattta gtagcatgtc attttctgaa ttatctttac
    21781 ctaattgtaa atcctcaatt tcagctgctt ttatttctac tctgtcttgt tcgttatttt
    21841 cttccacatt attaggtacc acatctttat tgctagctaa tattttgttt acgcagaata
    21901 taatttctct ttttaattct cttttcgcat ccgcttgatt tttaagaaat aaatctccac
    21961 attctttctt ttctaaactt atttttctaa gagatatatt aagaaggtct ttatatagat
    22021 ttaaaacctt ataatccttt tctaaacgtc ttcttctctc tgatagttcc attttatcat
    22081 aagctaaaaa actatttaaa tcattggctt ttagagagtc taatccatct ttcatagcct
    22141 tatttacata ctttctacat tttgctgtag ttaattctaa atctttttct aattcatcta
    22201 tttcaggtat gtacttatct actatccatt tgccaataag tggttctgta tttcttgcta
    22261 atgtcttata aaacaatata ttaaatttta aaagctctgc atattttgct tcattaaatt
    22321 ctactttttc aaatttcttt tccaaaacac catctagctt ttcatttatt ttagactctt
    22381 gttttaaaaa acttgctgtg acatttaaaa cagtgtttct tattatatta ttttcactca
    22441 aaaccttcac tccttaaatc aattatttta ttgtaatatt tacattgtgc aaagttgcta
    22501 atttgattat gatatctaaa taattattta ctaaatttgc tacatcttat atcatatcat
    22561 attatttaca tcattttact ttttcttttt atatctatct tatattatat catttttaat
    22621 atctgtacta acttatatta caatattcct caaaatttta aaagtagatt agacaaattt
    22681 acaaataaaa aaacatcagt ttaaactgat gtttttgact atcgaaataa ttaagcaagt
    22741 ctataagcgg agttctgtat tagataatca tctttctagg cctattgtca ccaataggct
    22801 caagcgacct acctaccgga gagtacgagc agcactcttt attcctatct tggtcttgct
    22861 ccaggtgggg tttacatagc caactagtcg cctagctgct ggtgagctct tacctcgcct
    22921 ttccatcctt accacaatag tggcggttta tttctgttgc actatcctta ggatcgctcc
    22981 cactaggcgt tacctagcac cctgctctat ggagctccga ctttcctcat gtaagattac
    23041 atgcgattat ctggcttact taatatttat tttacttagt tatgttaaca caaattcttt
    23101 tacatgtcaa agtaatatta tactaaaaat ttaaatttat tcaaaaatag tgtaatatag
    23161 atatataaac tacatttaac tcgaggtgat atattgaaaa aattatatag aataataatt
    23221 aacattatac tagtattagt aatactttac agtggattta atatatactc aaaacttacc
    23281 aaatacaatc atgatactaa aatatcctca gaacttcaaa aaaaagaata taaaaaagag
    23341 gacttatcaa agattaacag tgattttaag ttttggcttt ctgttgagaa tacaaatata
    23401 aattatccag ttgtacaatc taaagataat tcttactatc ttgacaaaga tttttataaa
    23461 aaagattcta tttctggtac actgtttatg gattatagaa ataaatctat tgatgacaaa
    23521 aatatcataa tatatggaca taatatgaaa aataaaacta tgttcaacaa cctaaataag
    23581 tttaaagatg ctgacttctt taaaaaaaat aataaaataa aaataacctt gaatggaaaa
    23641 gaatttttat atgatgtttt ttctgcttat attgtagaat ctgattatga ttatctaaaa
    23701 acaaacttta acaatgaatc tgattatcaa aattacataa atgatattac ttctaaatca
    23761 ttgtataaat ctcctgttaa ggtaaatagt aatgataaaa tagttacact ttctacatgt
    23821 acctatgaat ttgatgatgc cagaatggtg attcatggta gattggttta aaactcacag
    23881 acacagttta aaacaataat tttaaactgt gtcatatttt atacctaata atttataaat
    23941 taggctacta aaagtgtgta atacaaaata acaaacacat caaacaataa aaaaagacta
    24001 tgctaaaaac atagtcttta ctggagctgg tgataggaat cgaacctaca acctgctgat
    24061 tacaagtcag ctgctctacc gttgagccac accagcatac ctattctatt gtaaattagt
    24121 aaaactaatt tttaaagtta acactctaaa aactgcacat atatcttaat gaat
//
LOCUS       DAEPXF010000048        23348 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain HB27
            SAMN13376389-rid12479423.denovo.050, whole genome shotgun sequence.
ACCESSION   DAEPXF010000048 DAEPXF010000000
VERSION     DAEPXF010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13376389
            Sequence Read Archive: SRR10518432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 23348)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23348)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001069624.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001069624.1
            Genome Coverage       :: 315x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/15/2021 01:54:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,165
            CDSs (total)                      :: 4,076
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 89
            rRNAs                             :: 10, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 10 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 19 of 69
            Pseudo Genes (incomplete)         :: 44 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 10 of 69
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23348
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13376389-rid12479423.denovo.050"
                     /strain="HB27"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="China: Hebei"
                     /collection_date="2017"
     gene            274..1212
                     /gene="pdaA"
                     /locus_tag="KRL67_001637"
     CDS             274..1212
                     /gene="pdaA"
                     /locus_tag="KRL67_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="delta-lactam-biosynthetic de-N-acetylase"
                     /protein_id="HBH1522522.1"
                     /translation="MFRKVLHYLTLLSISIFFIVGCSNSQNNQNENQKKETQLQEDNE
                     KIDSGKDTSNVIVSDGTDKPSKGTANNDNNKLDVSSLDNTALDWFYIPNNKHKTPEVN
                     TDIEFKFSDYDAIYNGPTKDGQKTLYLTFDEGYENGYTTKILNTLKQNQVKAVFFVTY
                     PYIKENKDLVKRMVSEGHIVGNHSKTHPSMPTKTSNLENFNEELYDVEKLYKDVTGKD
                     IVKFFRPPMGKYSEKSLAMTKNLGYKTVFWSFAYRDWDTDKQPSHEEATQKIMDNLHD
                     GSILLLHAVSKTSTEILNDFISNARKLGYEFELLEY"
     gene            complement(1572..2945)
                     /locus_tag="KRL67_001638"
     CDS             complement(1572..2945)
                     /locus_tag="KRL67_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH1522523.1"
                     /translation="MVLTGAHYIYILFMVIILITMIMKKDTIVPCILGVFFMGLFFEK
                     NIFGAITAVFNSFIISLNELGPIILIIAIMVALSKALEANNAIQYMVRPFSRVIKNSN
                     TAFFVTGFVMLVLSWFFWPTPAVALVGAVFLPVAMRAGLPAIGVAVALNLFGHGLALS
                     TDFVIQGAPSITAGAAGVAVSYVINDGMILFWVMGIVTISMAFYTLKRDINKGMFREE
                     LKGFESEEVEEFNGKSKIATILVALGFLADIIAMYVFDLKGGDASALLGGTAVFLIII
                     INVINFGKDSLENVCENIIDGFVFGIKIFGAIIPIAAFFYMGEVAPLTGVFGKVLAPG
                     SQGLLSDIGIALSQTIPLNKVAVSGIETVVGAITGLDGSGFSGISLVGSLASVFGTAI
                     NASVGALAALGQISGIWVGGGCLVPWGLISAAAICGVSPIELAKRNFIPVITGLVVTT
                     IVAMFII"
     gene            complement(3132..4454)
                     /locus_tag="KRL67_001639"
     CDS             complement(3132..4454)
                     /locus_tag="KRL67_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBH1522524.1"
                     /translation="MISKIKPSKLGVILLIYVVISAAVAFILNIPFYSAVESIVKDVS
                     EGKVYFKREVNQLMDDFQTYVNNNGISADDGDDIAKWNGDNWFVSMKIYKGNKVFYDT
                     LYYPNEVPDKSEENEFYSSPMARNYEIQFSDIKANIVVVALFRFRFQNFIEAISITFM
                     ALTFIITFLLLFSRKIKYLRKIEMGIKIVESGSLKYRIPVKGNDELSSLASSINEMSH
                     TLENQLIKEREMKNKNKDIVTSISHDVRTPLTSVICYLDLIKDHKYNSPEQLEQYIKN
                     VRSKAYQIKDLTDDLFTHFVNSGEEVSKGYEKINANEFIFQLLLDSSYTLEEKGFHIT
                     IENELYNQYHIYVDVTQFRRIFDNLCSNIIKYARKDKPVVFLIKVEDSKLLIIQKNRV
                     SNFSKNVESFGIGIKNCEKIVAYHNGSINVNSDDKEFAIEISIPAKES"
     gene            complement(4447..5151)
                     /locus_tag="KRL67_001640"
     CDS             complement(4447..5151)
                     /locus_tag="KRL67_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBH1522525.1"
                     /translation="MNKEIPKKILVADDNPEIREIVEILLTGEGYEVIMATNGEEAVN
                     LVDSTIDLIILDVVMPVKTGFVACNEIREKTTAPILFLTAKTQDSDKVIGFSAGGDDY
                     LSKPFSYSELISRVKSLLRRYYIYQGKEKEREPNILKTKELCVNLDTQDVTLNGEPVF
                     LTTIEYSILILMLKNRKKVFSSENLYETIWDEQYFYTANNTIMVHIRNLRKKLEKDQK
                     NPQYIKTAWGKGYYID"
     gene            complement(5334..6398)
                     /locus_tag="KRL67_001641"
     CDS             complement(5334..6398)
                     /locus_tag="KRL67_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase-like domain-containing protein"
                     /protein_id="HBH1522526.1"
                     /translation="MISKTEKKNKCLGSVAFLVLLMGITGYFVFRGQSVESLIKSLKG
                     ASPMFILIGFAMMFIYVACEGINIYLGMKALNQKTTLLKCMGYAFIGFYFSSITPSAS
                     GGQPAQVYYMKKDDINISYSSLILLVIVVIHQVVILAYSGIMFIMEREFILNNVSGMN
                     ILLIYGVITNVALVVGVIAIIFSKKLVNNFIISITNLLGKLRIIKDVENSRKVITSQI
                     EEYVKGAQYIKQNPKLVVQILVITIIQITAMFLVPFFVYKAFHLSTYTVFEILAIQSL
                     LNIAVSSLPLPGAVGASENSFMTLFKIFFPGHLLVPAMLLSRGISFYAFVAISGLICI
                     VVHVKSSEKVKAVKKVAYVR"
     gene            complement(6401..7099)
                     /locus_tag="KRL67_001642"
     CDS             complement(6401..7099)
                     /locus_tag="KRL67_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HBH1522527.1"
                     /translation="MYVVGLIIVVALIFLVHSIIPTYYNKLLNKEVLKNVAGENEIAL
                     TFDDGPDKRYTEKLLDVLKENDIQAMFFVVAKNAEKEPEIIKRMLRENHIVGLHSLEH
                     KNAWLYSYSYVKKDFIESTNIMKNLGVDVNYYRPPWGHTNIFSNSFVKKYNLKMTLWD
                     VMAEDWEKDSTVDIIINKLMSRTKENSIICLHDAGENSGGAVGAPERTIEALKIAIPK
                     LKASGLKFVTPERM"
     gene            complement(7090..8187)
                     /locus_tag="KRL67_001643"
     CDS             complement(7090..8187)
                     /locus_tag="KRL67_001643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-diphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HBH1522528.1"
                     /translation="MKVLILTGKFGMGHYSASNSLSEDIKAKFDNSEITIKDIFEYIM
                     PNYSDKMYKTFSILVNRGSSLYNLFYKCAENGKKDIKFTFSDYFLNKLDTLLNEVQPT
                     VVISTFPFCSQLVSRYKEKYNSNLPLITCITDISSHSEWISKNTDCYLVASKSTKEEL
                     VFKGIDESKIKVNGIPVKKEFKRIEHINHSTKKNILIMGGGLGLLPKSEQFYRELNGL
                     EGVKTTVITGNNKKMYYKLYGRYENIEVVGYTNEVYKYMKDSDLIISKPGGITLFETI
                     YSELPILAFNPFLQQEIDNASFILNNEIGRILGKNKKYYVDEIKDLIYDDVTLKEMSS
                     NMKELKKQFDNNTLENILFSLDEQGACRECM"
     gene            complement(8512..9756)
                     /locus_tag="KRL67_001644"
     CDS             complement(8512..9756)
                     /locus_tag="KRL67_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HBH1522529.1"
                     /translation="MFENLKKADPEIYESMKRELKRQQRNIELIASENIVSVPVMETM
                     GSHLTNKYAEGYSGKRYYGGCEYIDEVETLAIDRIKKIFGAEHANVQPHSGANANIGV
                     YFAMLKPGDVVMGMNLSQGGHLTHGAPVNISGQYYKFYEYGIDKDSGLIDFDEVRKIA
                     HEVKPKMIVAGASAYPREIDFKKFREIADEVGALLMVDMAHIAGLVAAGLHQNPCEVA
                     DFVTTTTHKTLRGPRGGVILCKEKYAKDIDKAIFPGIQGGPLEHIIASKAVCFKEALS
                     DEFKEYQVQVAKNAKALAEELIKRDFKLISGGTDNHLILLDLTNKNITGKAAEKRLDD
                     AYITANKNTIPFDPNGALVTSGIRLGTPAVTTRGMKEEDMAIIAEAIDLCLTYDEESK
                     ARTLVVGLTEKYPLYEEYNIMD"
     gene            10084..10872
                     /locus_tag="KRL67_001645"
     CDS             10084..10872
                     /locus_tag="KRL67_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HBH1522530.1"
                     /translation="MNQNEELEYKKNILKLALFVGELMIKNGAETSRVEDSVLRICRS
                     RGFYHVNIFTTPTVIIISDEKFDGFSFMKTIQSRGINLNKISLLNSFSREFVSDKEYD
                     IDNAIARLYEIQDVKPYPLWLFYSCTGIASACFACLLGGNYVLNFILTFIIAIFAAIT
                     YDKTMKISAISAFSCLVSSFLIALSGVLLVEIGILDDPKMLIVGAIMPLLPGVAFIKA
                     IRDLISGNLISGISRAFDAAMIIIAIASGVGFVLDTWYRIGGPF"
     gene            10872..11345
                     /locus_tag="KRL67_001646"
     CDS             10872..11345
                     /locus_tag="KRL67_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HBH1522531.1"
                     /translation="MTDMSMILHFLFSGVATAGFAVFFNAPLYLLLPAGITGGIGWIV
                     YVYLFNFTTNAVFAGFIAAALVSACSETLARKLKQPAIVFVIPGILPLIPGIGLYNTM
                     LSLIQKNYSLAMSKGTDALFLSAAIALGVLVVTSFVRTLNLLKIRKNFIPYRKSK"
     gene            complement(11437..11877)
                     /locus_tag="KRL67_001647"
     CDS             complement(11437..11877)
                     /locus_tag="KRL67_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SoxR reducing system RseC family protein"
                     /protein_id="HBH1522532.1"
                     /translation="MNQQGFIMEIVDDRTAKLKMQRHSACAACGKCATTNSAESKEIL
                     VEVDNTIGAKVGDHVEVSMDNMNVLKAAAMAYIIPLIALLVGTIGTYYILGAIGMTTG
                     VEAISGLVGICLTIICYLYLKKNDKKFRDSREFIPVITRILIDL"
     gene            complement(11892..12185)
                     /locus_tag="KRL67_001648"
     CDS             complement(11892..12185)
                     /locus_tag="KRL67_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HBH1522533.1"
                     /translation="MEERKLAPNNEREALIKRLKRIEGQVKGIQKMVEQERYCVDILV
                     QISAIRSAINRVGTIILENHIKGCVAESIKHDDADKTEEMIKELMDTINKFTK"
     gene            complement(12228..12629)
                     /locus_tag="KRL67_001649"
     CDS             complement(12228..12629)
                     /locus_tag="KRL67_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH1522534.1"
                     /translation="MKKCLYCNRDIKSPNNKLAIKYDDNMNIYPCRAECKEKIEEYIA
                     KKPTYKFINILEVLLGVGGIFCFLSKWTIAAQVVLGIDALVIFLFPLAGFKMNGKLSM
                     EQTKNQSRSIAISILAFIVIITVLYFKQVGK"
     gene            12862..14982
                     /locus_tag="KRL67_001650"
     CDS             12862..14982
                     /locus_tag="KRL67_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBH1522535.1"
                     /translation="MKQMELAIVSLKKDAGEIYENQIRQFLGDNLKINLYSFEEGNLK
                     FFKEKLILLSAYLKYDEIVKLSRYDAQIIVPKLTFEKNSIDMISKLEKDKIIYVYNLS
                     KDMAIETISLIHRLGIDNINVLPCYPEIEFTPTDAVILTPGEKILPKFKNCEVIDLKY
                     RIIDLSCIVEIATKTKLKHLIKDDLIKKYVEKIIPTSYSTGELLLDANKFERQFDLLL
                     SIIDDGIICTNNDGIIQFYNHMARKILSINANEMIDSFVGDCIKDINFQNILTNKTPF
                     FEKLIKINHIDINLEIKHIQLNVFDGFILKMTKFSQLEKKQAKLRAQLVNSGNISKYT
                     FDDILGSSIQTINTKKIANKMAQSNSSILIIGESGTGKELFAQSIHSASRRKDGPFVA
                     VNCSTFQENLLQSELFGYDEGAFTGAKKGGKIGLFELANNGTIFLDEIGEMDLNSQSK
                     LLRVIQEKQVRRIGSNNVIDIDVRIIAATNRNLKELVSKNMFRRDLYFRLNVLPLKIH
                     PLRERPADIFEIFGSLKYDIPCNFSLSEEVKEIFKMYRWEGNVRELRNLGEYFCYLGK
                     DIIEICDLPEYILDTIDSNYSRTVCNKVSDNIKKYQFNIGKDKNIMKYDYNFKRSLDE
                     YIFILDNLKKAYDLKERIGRKSLCKIALEENRFLTEQQIRNMLLELQDFGLVDILVGR
                     GGSIITSKGVEFLKNINRSNKLNS"
     gene            15207..16373
                     /locus_tag="KRL67_001651"
     CDS             15207..16373
                     /locus_tag="KRL67_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HBH1522536.1"
                     /translation="MNYNFNEIVDRSNNFSSKWSEMEKKYGTNDLLPMWVADMDFKAA
                     PCIIDSLKNRLEQEIYGYTTRPDSYNESIVNWLNRRHNWKIKSEWLIYSPGVIPAISL
                     LINELTKENDKIMIQEPVYSPFNSVVKNNNRELVISPLQKLENGNYIMDYEDIENKIK
                     DVKLFILCNPHNPVGRVWTKDELKKLGDICLKYNVKIISDEIHSDIILKKHKHIPMAS
                     ISKEFEKNTITCMAPTKTFNIAGLQSSYVILPDEKDYKLLDDAFTRIDIKRNNCFSLV
                     ATEASYNNGESWLESFLEYLESNIDFAIKYINENMPKLKVRKPEGTYLLWVDFNALGL
                     SDEELESILVQKGKVALNQGNSFGIGGSGYQRINLACPRSMLEEALIRIKNAIN"
     gene            16398..17840
                     /gene="nhaC"
                     /locus_tag="KRL67_001652"
     CDS             16398..17840
                     /gene="nhaC"
                     /locus_tag="KRL67_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HBH1522537.1"
                     /translation="MKMNTEKKIPSFRYSLLVMLAIIALTSVGIVVFNASITTMFLLS
                     WLIVVPAAMKLGYTNDEIEAFGFEVGKDAFQSNLIILSVGVLISAWIAAGTIPTVVYS
                     GLTIITPKYFLLTTLIVCSLTSVATGTSWGTLGTSGIAMMSIGTSMGIPLGLTAGAVI
                     SGAFFGDKMSPLSDSTNLAAAVCKTDVITHMKHMFYTTGPAYVICIVLYTVIGFKYSN
                     NTIDYIQINQIKDVLNSNFHIGLVAMIPIIFLLLLLLLQKPPIISILSSAIMGLIIAV
                     FQEGEKIGDVLNYMLSGFTIDTGFVYADKLLNRGGIMSMAETVLLVFVVFVIAGILQK
                     TGFLEVLLQPLINKIGKSRTKLVGSTFIVSYFANAFSSSMMFTSVFVGTLMSPIYKEF
                     KLKPQNLSRIIEDTATLGGPLIPWNSNAVFCAQTLGVSPLEFIPYCFLSWITPIISFI
                     YGVTGFTMLTYTDDEIEELEIFDTIQRRVD"
     gene            17843..18112
                     /locus_tag="KRL67_001653"
     CDS             17843..18112
                     /locus_tag="KRL67_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH1522538.1"
                     /translation="MRFEYYYLIQDIAGILLAFIGLRMSIIGFRILSMKGISINTLSI
                     VIKYCLFTIAGLNLLISKFGIRHWIWSVCMLLISIIINPRIKVSK"
     gene            complement(18188..19615)
                     /locus_tag="KRL67_001654"
     CDS             complement(18188..19615)
                     /locus_tag="KRL67_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp14"
                     /protein_id="HBH1522539.1"
                     /translation="MKLIKSISSMLCLSLVLSLSPVAYANEISRTAVENTLVGQDRYQ
                     TAVEVSKKGWNSANEAVVVNSESIVDALAVTPFAKLKNAPILLTEKNNLNSQTEKELK
                     RLGVKKVYIIGLSSSVSNDVQKQLESNNLATERVGGSDRYQTALSIAKKIEGLKDISE
                     IAIVNGYTGLADAVSIASVAATNGMVILPVSDTSGISSFKDFIESKNINKSYIIGSTN
                     AVSDKIKQSLPNSERIGGADRNETNGKVIEKFYTSNKLNNVFVAKDGMKKQNQLIDAL
                     AVGVLASKENSPVLIVGEQLSATQKSVFSNKNATSITQVGSGGNENAFKELKDLQANS
                     NNKRVMYVTNTDKVNIRNDATIEASVIGYLSNGDEVEVLDVLKTGWVRIKYNEGIGYV
                     SGSYLTNNKPNNSNENIKIKYVKEKDGLNVRKGPSTEDEKIGHLSYGSKVETIEMFAT
                     GWVKIKYNGGYGYVSNDYLSDTPVI"
     gene            19987..20856
                     /locus_tag="KRL67_001655"
     CDS             19987..20856
                     /locus_tag="KRL67_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBH1522540.1"
                     /translation="MDLLHLKYFQTVARMEHITKASHKLNIAQPALSKTISSLEKELG
                     VQLFDRKGRYIVLNEYGRLFLKRVDSILDLVESSKKELQDTSLENSGEVKILSPAAAN
                     VLPPLLSNFRKLYPNITFNVSHTLPSSYKKSDFDLYISSSFTKLNSENSITLTCEEIL
                     LGVSINHPLSLKDEVYLSEVSDENFIVITKGENYREVIDILCESANFKPKIAFESDSP
                     YTIYALIKSLQGVGFICGKSWGLSQDPEIKLLHIKDIEFKRYLNLSWFSENYESKAVL
                     LFKNFLINYFKNI"
     gene            complement(20968..21774)
                     /locus_tag="KRL67_001656"
     CDS             complement(20968..21774)
                     /locus_tag="KRL67_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase"
                     /protein_id="HBH1522541.1"
                     /translation="MGRKVKIGIIQQHSVLGDVKKNIEKAVEMIDDLGKQGADIICLP
                     ELFATGYNLESLGGVKTLELIREHNKYIEESMSEAAKRNNVYLISPYGTLEKGYTHVY
                     NSAVIFDRKGKIMGEYCKNHLWSLEAVYFKAGEKVEVYDADFGRFGVMICYDAGFPEV
                     SRELTLKGSEIIFIPSAWRIQDEDMWDLNVSQRALENTVYTVGVNLVSNDSNLILFGK
                     SKICNPRGTVITQLDTYREKYVLSEIDLDEIKRYREDIPYLKDRKSSYNI"
     gene            complement(21788..23020)
                     /locus_tag="KRL67_001657"
     CDS             complement(21788..23020)
                     /locus_tag="KRL67_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytosine permease"
                     /protein_id="HBH1522542.1"
                     /translation="MQEREELVLNPVPKEERVGWLAPLFNMLGSNIAISELMVGGTLI
                     LGMTLSNMIITSIIGNLILVAIIMIQGYIGYKEGLNTYVLAKGAFGEIGGKYLISLLL
                     GITSFGWFGVQAGVAGLSVQKIFPSVNLTLVTVILGLFMVVFALYGFKAMAKFNYLVI
                     PPLMVLMVWGVFKAFSTYGVEAIYNYTPQTTMSMVEGLNIVVGLVIVGAIISPDQLRY
                     TRRVKDIWIISFLGLGIISLFQQVAAGVMSMGAPTWDITEVLANLGFGWVAFVILILA
                     SWSTNVSNAYSGGLALKTIFPNVKRNVLTLVAGLIGTIIAATGIIFKFQSFLSFLGIA
                     VPAIAGIMWCEYYFIQGRTYKHREGINWIAVISWLIGFAASYYSSKINFLIPPINGIV
                     VSMVIYYILMKCFGIKDK"
BASE COUNT         8152 a         3695 c         2912 g         8589 t
ORIGIN      
        1 attcaattaa cttctttgtg cttgttattt tgttacttct tgttgagcac atttattata
       61 ataacatctt ctcataatat ggtcaactat ttttttcttt tttttaaaaa tttattgatt
      121 attcaccctt tgtcatacta ttgtgacttt atttttaaag atataactat tattaatagt
      181 aatttccaat tataataata ctatatgcaa ttattattaa tcatataatt attattatat
      241 gtaattatta ttaatttaga aaggagcttc aaaatgttta gaaaagtatt acattattta
      301 acactacttt ctatatctat tttttttatt gttggatgtt caaattcaca aaacaatcaa
      361 aacgaaaatc aaaagaagga aacacagtta caagaagata atgaaaaaat agatagtggt
      421 aaggatactt ccaatgtaat agtttcagac ggaacagata aaccatcaaa aggcactgct
      481 aataatgata ataataaatt agatgtatct tctttagata acactgctct tgattggttt
      541 tatataccaa acaataagca taaaacacca gaagtaaata cggacattga atttaaattt
      601 tctgactatg atgccatata caatggacct acaaaagatg gtcaaaaaac actatatctt
      661 acatttgatg aaggttatga aaatggatac actacaaaga tactcaacac cctaaaacaa
      721 aaccaggtta aagcagtttt ttttgttaca tatccatata taaaagaaaa taaagattta
      781 gtaaaaagaa tggtttctga gggacatata gttggaaatc atagtaaaac ccatccatct
      841 atgccaacaa aaacaagtaa tctagaaaac tttaatgaag aattatatga tgttgaaaag
      901 ttgtataaag atgtaactgg gaaagatata gttaaatttt ttaggcctcc tatgggaaaa
      961 tactcagaaa aaagcttggc aatgactaaa aatttaggat acaagactgt attttggagt
     1021 tttgcatata gagactggga tactgataaa caaccatcac atgaagaagc aacgcaaaaa
     1081 ataatggata atctacatga tggttctatc ttgttactac atgcagtttc caaaactagt
     1141 acagaaattt taaatgattt tatatcaaat gcaagaaaac taggatatga atttgaactc
     1201 ttggagtatt aagatttaga attacaacta aagtctacaa actagtatag ttaatataat
     1261 ataggttata aaataataaa ttaaaattag aatttttgtt tggtaatagt ataggtaaaa
     1321 aacttgtgat agcttatata tgttggtagg acaggtcaat aaaacatcaa aaaatttggc
     1381 aagatactaa atatagataa cagtatttta gaggttatat atagtataat aaaaaatatt
     1441 attttattaa agaatactaa taaaaatacc aataaaaata gatgtatggc ttagaattag
     1501 taaaacaatt atgataaatt atagccacac atctattttt aatttgtaca attaaatatt
     1561 tatttgattt attaaataat aaacattgct actatggttg ttacaaccaa tccggttata
     1621 actgggataa agtttcgctt tgccaattct attggactaa ctccacaaat tgccgctgct
     1681 gaaataagac cccaaggaac tagacagcct ccacctaccc atataccact gatttgtcct
     1741 aatgcagcta atgcaccaac acttgcattt atagcagttc caaatacaga ggcaagtgaa
     1801 ccaactaatg aaatcccaga aaatccagaa ccatcaagcc cagttattgc tcccactaca
     1861 gtttctatac ctgatacggc aactttatta agtggtattg tttgagaaag agctatgcct
     1921 atatctgata ataaaccttg tgaacctggt gctaatactt ttccaaatac ccctgtcaag
     1981 ggtgcaactt cacccatata gaaaaatgct gctattggta taattgctcc aaatattttt
     2041 atcccaaata caaaaccatc aattatattc tcacatacat tttcaagact atctttgcca
     2101 aaatttataa catttataat tattattaaa aatactgctg ttccacccaa aagagctgat
     2161 gcatctcctc cttttaggtc aaatacatac atagcaatta tatcagcaag aaatcccaga
     2221 gctactaaaa tagttgctat tttagatttt ccattaaact cttctacttc ttcgctttca
     2281 aaacctttta gttcttctct aaacattcct ttgttaatat ctctttttaa tgtataaaat
     2341 gccatagata tggttacgat tcccatcacc caaaacaaaa tcataccatc atttataaca
     2401 tatgatactg caacacctgc tgcaccagct gttatacttg gagctccttg aataacaaag
     2461 tctgtagaca aggcaagacc atgaccaaat aaatttaatg ctactgcaac accaattgct
     2521 ggtagcccag ctctcatagc aacaggtaaa aacacagctc caactaaggc aacagctgga
     2581 gttggccaga aaaaccaaga aagaactaac ataacaaatc cagttacaaa aaatgctgta
     2641 ttagaatttt ttataactct cgaaaatggt ctcaccatat actgtatagc attattggct
     2701 tctagggctt tagataatgc aaccataata gctataatca agatgattgg acccaactca
     2761 tttaaagata taataaaaga gttaaacact gctgttatag caccaaaaat gtttttttca
     2821 aagaacaatc ccataaaaaa aacacctaat atacatggta ctattgtatc ctttttcata
     2881 atcatagtaa ttaatataat taccataaat agaatgtaaa tataatgtgc tcctgtcaaa
     2941 accatattat tatgtcccct ttttttaaga taataatatt atatggtctt gtaagtaaac
     3001 ttgttacttg cctagaggta cacaatattg aacatcccat aactaaagtc acatgcttcc
     3061 tgcttcatag aattttacat aagcaattac acaatatata atagcaatta cacaatatat
     3121 aatttaaatt tttatgattc ttttgctgga atagatattt caatagcaaa ttctttatca
     3181 tctgaattta catttatgct tccattatga tatgcaacta ttttctcaca attctttata
     3241 ccaattccaa aactttctac attcttagaa aaattgctta ctctattttt ttgtataatt
     3301 aataatttag aatcctctac tttaattaaa aatactacag gtttatcttt cctagcatat
     3361 tttattatat tagaacataa attatcaaat atccttctaa attgtgtaac atctacataa
     3421 atatgatatt ggttataaag ttcattttct attgttatat gaaagccttt ctcctccaat
     3481 gtatatgaac tatctaaaag taattgaaaa ataaattcat tagcatttat tttttcatac
     3541 ccttttgaaa cttcctctcc agaattaaca aaatgcgtaa aaaggtcatc tgttaaatct
     3601 tttatctgat atgctttaga ccttacattt tttatgtatt gttccaattg ctcaggacta
     3661 ttgtatttat ggtctttaat caaatctaaa taacaaatta ctgacgttaa tggagttcta
     3721 acatcatgtg atatcgaagt tactatatct ttatttttat tcttcatctc tctttctttt
     3781 ataagttgat tctccaatgt gtggctcatc tcattaatac tagatgcaag tgatgataat
     3841 tcatcattac cttttactgg gattctatac tttaaactgc cactctctac tatctttata
     3901 cccatttcta ttttccttag gtactttatc tttctactaa acaacagtaa aaatgttata
     3961 ataaatgtaa gtgccataaa tgtaatagaa attgcttcta taaaattttg aaatctaaat
     4021 ctaaataaag ctactacaac tatattagct ttaatatcag aaaattgtat ctcatagttt
     4081 ctagccatgg gactgctata aaactcattt tcctcactct tatcaggtac ttcattagga
     4141 tagtacaatg tatcataaaa aaccttattt cctttatata tcttcataga aacaaaccaa
     4201 ttatcaccat tccattttgc tatatcatct ccatcatctg cacttatacc attgttattc
     4261 acataagttt gaaaatcatc cataagttga tttacttctc ttttaaaata tacttttcct
     4321 tctgatacat cttttactat agattcaaca gcagagtaaa atggtatatt taaaatgaaa
     4381 gccactgcag ctgagattac tacatatatc aatagtataa ctcccaactt agatggctta
     4441 attttgctaa tcaatataat acccctttcc ccatgcggtt tttatatatt gtggattttt
     4501 ttggtctttt tctaactttt ttcttaaatt tctaatatga accattattg tattatttgc
     4561 cgtataaaaa tactgctcat cccaaatagt ttcatataaa ttttcagatg aaaaaacttt
     4621 ttttctattt ttcaacataa gaattaatat actatattct atagttgtca aaaacacagg
     4681 ttctccattt agtgtaacat cttgagtatc taaattgaca cataactctt ttgtttttaa
     4741 tatatttggc tctctttcct tttcctttcc ctggtatata taataacgtc taagtagcga
     4801 cttaaccctt gaaattagtt cagaatatga aaaaggcttt gataaatagt catctcctcc
     4861 tgctgaaaat ccaataactt tatcagagtc ttgagttttt gcagttaaaa ataatattgg
     4921 tgctgtcgtt ttttctctaa tttcattaca tgcaacaaat cctgttttta caggcattac
     4981 aacatccaaa ataatcaaat caatcgtact atctactaag tttacagctt cttctccatt
     5041 cgttgccata ataacttcat aaccttcacc agttaaaagt atttctacga tttccctaat
     5101 ctcaggatta tcatcagcta ctaaaatttt ttttggaatc tctttattca tcgccaagct
     5161 cctttttttg ctatatttct taaatatttc aaactatata aaatattata ctttattagc
     5221 aaaagcttag caatgtcaca cgttttaata ctttctactc ctgtaaacag gtttaaaact
     5281 tcattaaaat gtatacacaa attgacatat taataaaaat aatctatata atattatctt
     5341 acataagcaa cttttttcac tgcttttact ttttcagaag attttacatg tactactata
     5401 catatcaatc cacttatagc tacaaatgcg taaaaactta ttcccctact aagcaacatt
     5461 gccggaacta ataagtgacc agggaaaaat atcttaaata atgtcataaa actattttca
     5521 gaagctccaa cagcccctgg cagtggtaat gaagaaacag caatattaag caaggattgt
     5581 atcgctaata tctcaaatac agtatatgtt gatagatgaa aagctttgta tacaaagaaa
     5641 ggaactaaaa acatagctgt tatttgtata atagttatga ctaaaatttg gactacaagt
     5701 tttgggttct gctttatata ttgagcacct ttaacatact cttcaatttg agatgttata
     5761 acttttcttg aattttcaac atcttttatt attcttaact tacctaataa atttgttatt
     5821 gatattataa aattgtttac aagttttttt gaaaatataa ttgcaattac tccaacgaca
     5881 agagcaacat ttgttattac accatatatt agtaaaatgt tcataccact tacattattt
     5941 aatataaact cacgttccat aatgaacatt attccactat atgccagtat aactacttgg
     6001 tgaataacaa caatgactag tagtataagt gaagaatatg atatatttat atcgtctttt
     6061 ttcatataat aaacttgtgc tggttgacca cctgatgctg aaggcgtaat agaactaaaa
     6121 taaaatccaa taaaagcata tcccatacat tttaaaagag tagttttttg atttaaagct
     6181 ttcataccta aatatatatt tataccttca caggcaacat atataaacat cattgcaaat
     6241 ccaattagta taaacatagg gcttgcacct tttaatgatt ttatcaatga ttcaactgac
     6301 tgccctctaa aaacaaaata tcctgttatg cccattagta aaactaaaaa agctacacta
     6361 cctaggcatt tatttttctt ttcagtttta cttatcataa ctacatcctt tctggtgtta
     6421 cgaactttaa accactagct ttaagttttg gaatagctat ttttaaggct tcaatagtac
     6481 gctcaggtgc acctactgca ccacctgaat tttcacctgc atcatgtaaa caaattatag
     6541 aattttcttt tgttctactc attaatttgt ttataataat gtctactgta ctatctttct
     6601 cccaatcttc tgccattaca tcccacaagg tcatttttag attatatttt ttaacaaatg
     6661 aatttgaaaa tatattagta tgaccccatg gtggacgata gtagtttaca tctacaccta
     6721 aatttttcat tatattcgta ctttcaataa aatctttttt aacataacta taactataaa
     6781 gccatgcatt tttgtgttcc aatgaatgta aaccaactat atgattttct ctcaacattc
     6841 tttttataat ttcaggctct ttttcagcat tctttgctac tacaaaaaac atagcttgta
     6901 tatcattttc ttttaacaca tctagtaatt tttctgtata tctcttatct ggaccatcat
     6961 caaatgtaag tgcaatttca ttttctccag ccacattttt taatacctct ttatttaata
     7021 gtttattata gtatgtaggt ataattgaat ggactaaaaa aatcaatgca acaacgataa
     7081 ttaaacctac tacatacatt ctctacacgc tccttgttca tcaagtgaaa ataaaatatt
     7141 ttctaaggta ttattatcaa attgtttttt taattctttc atgttactgc tcatttcttt
     7201 aagagtaaca tcatcataaa ttaaatcttt tatctcatct acatagtatt ttttattttt
     7261 accaagtatt ctcccaattt cattgtttaa tataaaacta gcattatcta tctcttgttg
     7321 taaaaatgga ttaaatgcca atattggaag ttctgaataa attgtttcaa atagtgttat
     7381 tccacctggt ttagaaataa taaggtcaga atctttcatg tatttatata cttcattggt
     7441 gtaacctact acctctatat tttcatatct accatatagt ttatagtaca tttttttatt
     7501 attaccagtt attacagtag tttttactcc ttctaaacca tttaattctc tgtaaaattg
     7561 ttctgattta ggcagtaatc ctaatcctcc acccattata agaatatttt ttttagttga
     7621 atgatttata tgttctattc ttttaaattc tttttttact ggaataccat taactttaat
     7681 cttagattca tctattcctt taaaaacaag ttcttcctta gtagatttac tagctactaa
     7741 ataacagtct gtatttttac taatccattc tgagtgacta cttatatctg ttatgcatgt
     7801 tattaaagga agatttgagt tatatttttc cttatatctt gatactaatt gagaacaaaa
     7861 tggaaaagtt gaaataacta ctgttggttg tacttcatta agtaatgtat ctaatttatt
     7921 taaaaaataa tctgaaaaag taaattttat atctttcttt ccattttcag cacatttata
     7981 aaacaaatta taaagactac ttccccgatt tacaagaata gaaaaagtct tgtacatttt
     8041 atctgaatag ttaggcataa tgtattcaaa tatatcttta attgttattt ctgaattatc
     8101 aaattttgct tttatatctt cacttaaaga atttgatgct gagtaatgac ccataccaaa
     8161 ttttcctgta agaattaata ccttcataag attccctcct cattaaagct tttcttttca
     8221 tataattact atacttataa gtatagactt taaatcttta gaaaaaccta gtataattct
     8281 taagaaatct taaagtttta attaaaaaat acaaagagac catctaaaaa tagagataac
     8341 cgtatttttt attatttaaa taacacaaat aggttcgatt acatacaaaa aaagtgcctc
     8401 atgaactaaa aagttcattt tgagacacct tttttatatc taaatatatt caaataaata
     8461 cacaatcatt gtaattattt aatatgtatt ttatttagtt gttcttcata attaatccat
     8521 aatattatat tcttcatata aaggatattt ttcagttaaa ccaacaacca atgttctagc
     8581 cttagattct tcatcataag ttaaacatag gtctatagcc tcagcgatta ttgccatatc
     8641 ttcttctttc atacctctag tagttacagc tggagtacca agtcttattc cacttgtaac
     8701 aagagcacca tttgggtcaa atggaattgt atttttatta gctgttatat aagcatcatc
     8761 caatctcttt tctgctgctt taccagttat atttttatta gtcaaatcta atagtattaa
     8821 atgattatct gtacctccag aaatcaattt aaaatctctt ttaataagtt cttcagctaa
     8881 ggcttttgca tttttagcaa cttgtacctg atattcttta aactcatcac ttaaagcttc
     8941 tttaaagcaa actgccttag aagcaattat atgctcaaga ggtccacctt gaataccagg
     9001 gaatattgct ttgtctatgt ctttagcata tttttcttta caaagaataa cacccccacg
     9061 aggtcctctt aaagttttgt gagttgttgt tgtaacaaag tcagccactt cacatggatt
     9121 ttggtgaaga ccagcagcca caagccctgc tatatgagcc atatctacca taagcaatgc
     9181 tccaacctca tcagctatct ctctaaactt tttaaaatct atctctctag gatatgcact
     9241 tgctcctgca actatcattt taggttttac ttcatgtgct atctttctaa cttcatcaaa
     9301 atctataagt ccactgtctt tatctatacc atattcataa aatttataat attgaccaga
     9361 aatattaact ggagcaccat gagttaaatg tccaccttga gataaattca ttcccataac
     9421 aacatcacct ggttttaaca tagcaaaata tacaccaata tttgcatttg ctccagagtg
     9481 aggttgaaca ttagcatgct ctgctccaaa tatttttttg attctatcta ttgcaagagt
     9541 ttcaacttca tctatgtatt cacatccacc ataatatctt ttgcctgagt aaccttctgc
     9601 atacttattt gtaagatgac ttcccatagt ttccattact ggaactgata ctatgttttc
     9661 agatgctatt aattcaatat ttctttgttg tctttttaac tctcttttca tactttcata
     9721 aatctcaggg tccgcttttt tcaagttctc aaacatatta ctcctcctag ttattattta
     9781 aattttattt ttacagacat ttcataattt tcgtttttat caatatgata atttattatt
     9841 atagtccttt accaattaat agtcaagtgt tttgccattt ttccatattt aacatagtta
     9901 ctttctttat ttttaagtta aaatataata aataatattg tctaattctt ttaattggat
     9961 ataatttaaa tgtgactatt ttgaaacaaa aaaaagataa tatatttata tagatattaa
    10021 ggaatattac ttaaaaattt gatataattt caatattacg ttttttaatt tggaggtgta
    10081 tttatgaatc aaaatgagga acttgaatat aagaaaaata ttctaaaact agctctattt
    10141 gtaggggaac tgatgataaa aaatggagct gaaacatcta gggttgaaga cagtgtcctt
    10201 agaatatgca ggtcaagagg gttttatcat gtaaatattt ttacaactcc tacagtaata
    10261 attatttcag atgagaaatt tgatggcttt tcgtttatga aaacgattca atcaagaggt
    10321 ataaacttaa ataagatttc tttattaaac agtttttctc gagaatttgt tagtgacaaa
    10381 gagtatgata ttgataatgc tattgcacgt ctttatgaaa tccaagatgt aaaaccttat
    10441 cctttatggt tattttactc ttgtacagga attgcatctg cttgttttgc ttgtttgctt
    10501 ggagggaatt atgtattaaa ttttatactt acgtttataa tagcaatctt tgctgcaatt
    10561 acttatgata aaacgatgaa gataagtgca atatctgcat tttcctgttt agtatcttct
    10621 tttttaattg cactttcagg agttctacta gttgaaatag gcattttaga tgaccctaaa
    10681 atgcttatag tcggcgctat aatgccacta ctcccaggag ttgcttttat aaaagctatt
    10741 agagatttaa tatcagggaa tttaatatca ggaatttctc gtgcttttga tgctgctatg
    10801 attattattg caattgcttc tggagttggt tttgtccttg atacatggta tagaatagga
    10861 ggtcctttct aatgactgat atgtcgatga ttttgcattt tttattttca ggtgttgcaa
    10921 ctgctggatt tgctgttttc tttaatgctc cactatatct acttcttcca gctggaataa
    10981 ctggcggaat tggttggatt gtatatgttt atttatttaa ttttactaca aatgcagttt
    11041 ttgcaggttt tatcgctgct gctttggtct cagcgtgcag tgagacttta gcaagaaaac
    11101 taaagcaacc agctattgtg ttcgtaattc caggtattct tcctctaata ccaggaattg
    11161 ggctatataa tacaatgttg tctttgattc aaaaaaatta ttctttggct atgtcaaaag
    11221 gtacagatgc actatttcta agtgctgcaa tagcacttgg tgtattagtt gtaacttctt
    11281 ttgtaagaac attgaatctt ttgaaaatca gaaaaaactt tattccttac agaaaatcaa
    11341 aataaaaata taaaaatagc aatgtactta aactctacaa atatgtattg agtatttgtt
    11401 taaaaagcac attgctattt tttaatcata attatcttaa aggtcaatta atattcttgt
    11461 tattactggt ataaactctc tactgtctct aaattttttg tcattcttct ttaagtataa
    11521 ataacagatt attgtaagac aaattccaac taatccactt atggcttcaa ctccagttgt
    11581 cattccaatt gcccccaata tataataagt ccctatggtt cctacaagaa gtgctataag
    11641 aggtattata taagccattg cagctgcttt taatacattc atattatcca tactcacttc
    11701 tacatggtca ccaacttttg caccaattgt attgtctact tctactaata tttcttttga
    11761 ctctgctgaa tttgtagtag cacattttcc acaagcagca caagctgaat gtctttgcat
    11821 ttttaacttt gcagttctat catcaacaat ttccataata aatccttgtt gattcataac
    11881 taaccgcctc cttattttgt aaatttattt atagtatcca taagctcttt tatcatctct
    11941 tcagttttat cagcatcatc atgtttaata ctttctgcta cacatccttt tatatgattc
    12001 tctaagataa ttgtgcctac tctatttata gcagacctta tagcagatat ttgaaccaat
    12061 atatcaacac aataacgttc ttgttccacc atcttttgta tacccttaac ttgaccttct
    12121 attcttttta atctttttat gagagcttcc ctctcattgt ttggtgctaa ttttctctct
    12181 tccataaact cacctcatac tctaatgatg tcttatacaa ttatgctcta cttaccaact
    12241 tgtttaaaat ataatacagt tattattact ataaatgcca aaattgatat tgcaatagac
    12301 ctactttgat tcttagtttg ttccatactt aactttccat tcatcttaaa tcctgccaat
    12361 gggaataaaa atataaccag tgcatcaatt ccaagtacaa cttgggctgc aatagtccat
    12421 ttactcaaaa aacagaatat tccgcccaca cctaaaagaa cctctaatat atttataaac
    12481 ttatatgtag gtttttttgc tatatattct tcaatttttt ccttacattc tgcacgacat
    12541 ggatatatat tcatattgtc atcatatttt atagctaatt tattatttgg tgactttata
    12601 tccctattac agtaaagaca ttttttcatt ttatattctc ctctagtatt ccttaaaaaa
    12661 ttttgcctaa attattatac gctctaatac tattatacca aatagataca aaaaacaaaa
    12721 ataaaaggaa taattttagc tatttctttt acttcaatgt gtagtctata ataaatttat
    12781 aataataatt aaataatatt atttaatcta atttaattat tatttaatct aaaataagtc
    12841 aaatttgtat gagaggttaa aatgaaacaa atggaattag ctattgtaag tttaaaaaaa
    12901 gacgctggag aaatttatga aaatcaaata aggcaattct taggtgataa tctaaagata
    12961 aatttatact catttgaaga gggaaattta aagtttttta aagaaaaact tatactccta
    13021 tctgcttatt taaaatacga tgaaatagta aaattatctc gctatgatgc tcaaatcatt
    13081 gttcccaagt tgacatttga aaagaacagt attgatatga tatccaaatt agaaaaagat
    13141 aaaattattt atgtatataa cttaagtaaa gatatggcaa tagaaacaat ttctttaata
    13201 catagattag gaatagacaa tataaatgtc ctgccctgct atccagagat tgagtttact
    13261 cccacagatg ctgtaatcct aactcctggt gaaaaaatct tgcctaagtt taaaaattgt
    13321 gaagttatag atttaaaata cagaataata gatttaagtt gtattgttga aattgctaca
    13381 aaaaccaaat taaaacatct aataaaagat gacctaataa aaaaatacgt agaaaaaata
    13441 attcctacaa gttatagtac aggtgaatta ttgttagatg caaataagtt tgaaagacaa
    13501 tttgatttgt tactatctat aattgatgat ggaattatat gcacaaataa tgatggaata
    13561 attcaattct ataatcatat ggctagaaaa attttatcta taaatgcaaa tgaaatgatt
    13621 gactcatttg ttggtgattg cataaaagac atcaattttc aaaacatatt gaccaataaa
    13681 actccatttt ttgagaaatt gattaaaata aatcatattg acataaattt agagattaaa
    13741 catatacaat taaatgtatt tgatggattt atactaaaga tgacaaagtt ttctcaatta
    13801 gaaaaaaagc aagctaaatt aagagcacaa ctagtaaact ctggtaatat cagtaaatac
    13861 acatttgatg acatattagg ttctagtata caaactatta atacaaaaaa aattgctaat
    13921 aaaatggctc aatcaaattc atctatcctt ataattggtg aaagtggtac tggtaaagaa
    13981 ttatttgctc aatccattca tagtgcttct aggagaaaag atggtccttt tgtagcagta
    14041 aattgtagta cttttcaaga aaatctactg caaagtgaat tatttggcta tgatgaaggg
    14101 gcttttactg gtgctaaaaa aggtgggaaa attggtttat ttgaattagc caacaatgga
    14161 actatatttt tagacgaaat tggagaaatg gatttaaatt ctcaatctaa attacttaga
    14221 gtcatacaag aaaaacaagt acgacgtatt ggttctaata atgtcattga tattgatgta
    14281 agaataattg cagctactaa tagaaattta aaagaacttg tatctaaaaa tatgtttaga
    14341 agagatttat attttaggtt gaatgttctt ccccttaaaa tacatccact tagggaaaga
    14401 cctgctgata tatttgaaat ttttggttca ttaaaatatg atattccatg taattttagt
    14461 ctaagtgagg aagtaaaaga aatttttaaa atgtatagat gggaaggaaa tgtaagagaa
    14521 ttaagaaact taggagaata cttctgttac ttgggaaaag atattataga aatatgtgat
    14581 ttacctgaat atatattgga tactatagat tcaaattata gcagaaccgt ttgtaataaa
    14641 gtaagtgata atataaaaaa gtatcaattc aacattggca aagacaaaaa catcatgaaa
    14701 tacgactata attttaaaag gtcactagat gaatacatat ttatactgga taatttaaaa
    14761 aaggcttatg atttaaaaga aagaataggt agaaaaagct tatgtaaaat agctttagag
    14821 gaaaatagat ttttgacaga acaacagatt agaaatatgc ttttagaact acaagacttt
    14881 gggcttgtag acatacttgt tggtcgtgga ggttcaatta taacatcaaa aggagtagaa
    14941 tttttaaaaa atataaatag gtctaacaag ttaaacagtt aaacctattt aacctaatta
    15001 attgcatttt catatattta aaaatttatt agaaataacc ttttccacaa agttttaagt
    15061 ataattaata tttttatctt atctatgctc tgaaattgct taatattaaa tattaacttg
    15121 tgaataaata ttttttatta tcaatattgg cacgttgttt gcttaatatt attaacataa
    15181 aattaaaatt actggaggta tttacaatga attataattt taatgagatt gttgatagaa
    15241 gcaataattt ttcatctaaa tggtcagaga tggaaaaaaa gtatggaact aatgatttac
    15301 ttcctatgtg ggtggctgac atggatttta aggctgcacc ttgtataatt gattccttaa
    15361 aaaatagact agaacaagag atttatggct acacaacaag accagattct tacaatgaat
    15421 ctatagtaaa ttggctaaat aggagacata actggaaaat aaaaagtgag tggttaattt
    15481 atagtccagg tgttatccca gctataagtc ttttaataaa tgaactcaca aaagaaaacg
    15541 ataaaataat gatacaagaa ccagtttaca gtccttttaa tagtgttgta aaaaataaca
    15601 atagagaatt agttataagc ccattacaaa aactagaaaa tggaaactac ataatggatt
    15661 atgaagacat tgaaaacaag attaaagatg taaaattatt catactttgt aatcctcata
    15721 atccagttgg tagagtatgg acaaaagatg aattaaaaaa attaggagat atttgtctta
    15781 agtataatgt aaaaatcata tctgatgaaa tacatagtga cattatatta aaaaaacata
    15841 agcatattcc tatggcatct atttctaaag aatttgaaaa aaatacaatt acttgtatgg
    15901 ctccaactaa aacttttaat atagctggtc ttcaatcatc atatgtaata ctacctgatg
    15961 aaaaagacta taaattgtta gatgatgctt ttaccagaat tgatataaag agaaataatt
    16021 gctttagctt agttgcaact gaagcttcat ataataatgg tgaatcttgg ctagaatcat
    16081 ttttagaata tttagaatct aacatagatt ttgctataaa atatataaat gaaaatatgc
    16141 caaaacttaa agttagaaaa ccagaaggta cttatttatt atgggtagac tttaatgcac
    16201 ttggattaag tgatgaggaa ctagaaagta tacttgtaca aaaaggtaaa gttgctttaa
    16261 atcaaggaaa cagctttggc attggaggaa gtgggtatca aagaataaac ttagcttgtc
    16321 caagaagtat gcttgaagaa gctcttataa gaataaaaaa tgcaattaac taaaaatatt
    16381 taaggagaat ttgcactatg aaaatgaata cagaaaaaaa aataccttca tttagatatt
    16441 ccttgttagt aatgcttgca ataatagctc taacatcagt tggtatagtt gtatttaatg
    16501 cttctataac tacaatgttt ttactttcat ggcttatagt agttcctgca gccatgaaac
    16561 ttggttatac taatgatgaa atagaagctt ttggatttga agttggtaaa gatgcctttc
    16621 aatcaaactt aattatactt tctgttggtg tactaatatc agcttggata gctgctggta
    16681 caataccaac agtagtatat agtggactaa caataataac ccctaaatat tttctgctta
    16741 caacattaat cgtatgttca ctaacttctg ttgctactgg tacatcttgg gggactcttg
    16801 gaacttctgg tatagcaatg atgagtattg gtactagtat gggtattcct cttggtttaa
    16861 cagctggtgc agtcatttct ggtgctttct ttggagataa gatgtcacct ctatcagatt
    16921 ctacaaactt agctgcagca gtttgtaaga cagatgtaat tacacatatg aagcatatgt
    16981 tttataccac aggacctgca tatgtaatat gtatagttct atatacagta attggattta
    17041 agtattcaaa taatactata gactatattc aaataaatca aatcaaggat gtattaaatt
    17101 caaactttca tattggttta gtagcaatga ttccaataat atttttatta ttacttttat
    17161 tattacaaaa accacctatt atatctatac taagttcagc tatcatggga cttattatag
    17221 cagtatttca agaaggagaa aaaataggtg atgttttaaa ttacatgcta tcaggattta
    17281 ctatagatac tggatttgtc tatgccgata aactattaaa tcgtggtgga ataatgagta
    17341 tggctgaaac cgttttacta gtatttgtag ttttcgttat tgctggtatt ctccaaaaaa
    17401 ctggattctt agaagtacta cttcaacctc ttataaataa aataggaaaa tcaagaacta
    17461 aattggttgg gtctactttt atagtgagct actttgcaaa tgcatttagt tcgtcaatga
    17521 tgttcacttc tgtatttgtc ggaactctaa tgtctccaat atataaagaa tttaaattaa
    17581 aaccacaaaa tctatctaga attatagaag atacagctac acttggtggt ccattaatac
    17641 catggaattc aaatgctgta ttctgtgctc aaactcttgg tgtaagccca ttagaattta
    17701 tcccatattg ttttttaagt tggattaccc ctataatttc atttatttat ggtgtaactg
    17761 gttttactat gcttacatac acagatgatg aaattgaaga gttagaaata tttgatacta
    17821 ttcagagaag agttgattga atatgagatt tgaatactat tacttaattc aggatattgc
    17881 aggtatatta ttggcattta taggacttag aatgtctatt attggattta ggatactttc
    17941 tatgaaagga atttctataa atacactttc aattgtaatt aaatactgct tatttaccat
    18001 agcaggactg aatttattaa tatctaagtt tggaattagg cactggattt ggagtgtttg
    18061 tatgttgcta atatccataa taataaatcc caggattaag gtatcaaaat agaaataatt
    18121 attagactta aatttttata aaaaaactgc tttacaaatt aaagcagttt tttgttgtta
    18181 cttataatta aataaccggt gtatcagata ggtaatcatt acttacataa ccatatccac
    18241 cattatactt tatttttacc catccagtag caaacatttc tattgtttct actttgcttc
    18301 catatgaaag gtgacctatc ttttcatctt ctgtacttgg tcctttccta acatttagac
    18361 catctttttc ttttacatac tttattttta tattttcatt gctattatta ggcttattat
    18421 ttgtcaaata agacccacta acatatccaa taccttcatt gtattttatt cttacccatc
    18481 cagtttttaa cacatctaaa acttctactt catcaccatt acttagatat cctataacag
    18541 aagcttctat tgtagcatca tttcttatat ttaccttatc tgtattagtt acatacatta
    18601 ctctcttatt attactattt gcctgtaaat cttttaattc tttaaatgca ttttcatttc
    18661 ctccactacc aacttgagtt atagaggtag catttttatt cgaaaataca ctcttttgtg
    18721 ttgcacttaa ttgttctcct acgattagta caggagaatt ttcttttgat gccagtactc
    18781 ctactgctag agcatctata agttgatttt gctttttcat tccatctttg gctacaaata
    18841 cattatttaa tttattagat gtataaaact tttctatcac tttaccatta gtttcatttc
    18901 tatctgctcc acctattctt tctgaattag gtaaactttg ttttatttta tctgatacag
    18961 cattagtaga gcctattatg tatgatttat ttatattctt agattctata aaatctttaa
    19021 agctagatat accagatgta tcagatactg gaagtataac cattccatta gtagcagcta
    19081 cagaagctat actaactgcg tctgcaagac ctgtataccc atttactata gctatttctg
    19141 atatatcttt taatccttct atttttttag ctatactcaa agctgtttgg tatctatcac
    19201 taccaccaac tctctcagta gctaaattat tagattctaa ctgtttttgc acatcatttg
    19261 atacagaact agatagacca attatataaa cttttttaac tcctaatctt tttaattctt
    19321 tctcagtttg tgaatttaga ttattttttt ctgtcaataa tataggagca ttttttaact
    19381 tagcaaacgg tgttacagcc aatgcatcta caatagattc actatttaca actacagcct
    19441 catttgcact attccatcct tttttgctta cttcaacagc agtttgatat ctatcctgac
    19501 ctactaaagt attctcaact gctgttctac ttatttcatt tgcatatgct actggtgata
    19561 aacttaaaac tagagataag cacagcattg atgatattga tttaattaat ttcatcttga
    19621 ctccccctaa aattatagta tataaatgtt tcctatttaa aatagtataa cataataatt
    19681 taagtaacat cgttattata aaatattatt tttcttatct aataagcttt tattcttact
    19741 tgacattaat gctttactca tcaaatgaat tttttatata actaaagttt gtaacaacat
    19801 ttccttagtt aatcatttaa agctactttt tttaattaga ttatgtacaa aatataaata
    19861 acaatttaaa taacttttta atatattgta gtttattata taaactttat attataatgt
    19921 ataatgtgga gttgattata aaattgatat aactcaagtc atattaaaag aggtgtattt
    19981 tatactatgg atttattgca tcttaagtat tttcaaactg ttgctagaat ggagcacata
    20041 acaaaagcat cacacaagtt aaatatagca cagccagctt taagcaaaac tatatcttcc
    20101 ttagaaaaag aacttggggt tcagttattt gacagaaaag gaagatacat tgttttaaat
    20161 gagtatggaa gattgtttct aaaaagagta gatagcattt tagatttagt agaaagttca
    20221 aaaaaggagt tacaagacac ttctttagaa aattcaggag aagtaaagat attatctcct
    20281 gcagcggcta atgtgttacc accacttttg agtaatttca gaaaactata tcccaatatt
    20341 acttttaatg tgtcacatac tttgccatca tcttataaaa aaagtgattt tgatttgtac
    20401 atatcttctt catttacaaa gctaaactca gaaaatagca taactttaac atgcgaagaa
    20461 atactattag gagtttcaat aaaccatcct ttatcattaa aggatgaagt atatctaagc
    20521 gaagtttcag atgaaaactt tatagttata actaagggtg agaattatag agaggtaatt
    20581 gatattctat gcgaatcagc taatttcaaa cccaaaatag catttgaatc tgattcacca
    20641 tatacaattt atgctcttat aaaatcactt caaggagttg gatttatatg tggaaaatca
    20701 tggggattat ctcaagaccc tgagattaag ttgttacata tcaaagatat tgaatttaaa
    20761 agatatttaa atctttcatg gtttagcgaa aactatgaat ctaaagctgt gttgttgttc
    20821 aaaaactttc ttatcaacta ttttaaaaat atttaaatca aagttgataa tattaaagag
    20881 gatatatcaa agtataataa atttaatctt aatattgata catcctcttt ttatcatttt
    20941 atattgaact tgcattataa catagaatta tatgttatag ctactttttc tgtctttcaa
    21001 atatgggata tcttccctat acctttttat ttcatctaaa tcaatctctg ataatacata
    21061 cttttcccta tacgtatcta attgagttat tacagtacct ctaggattgc atattttact
    21121 ctttccaaat agtattaaat tagagtcatt acttactagg tttacaccta ctgtatatac
    21181 agtgttctct aaagctcttt gagatacatt taaatcccac atatcttcat cttgtattct
    21241 ccatgcagag ggtataaata ttatttcaga accttttaac gtcaattctc tggatacttc
    21301 tggaaagcct gcatcataac atatcattac accaaatctg ccaaaatcag catcatatac
    21361 ctctactttt tcccctgctt taaagtatac tgcttctaat gaccacagat gatttttaca
    21421 gtattctccc ataatcttac cttttctatc gaatataact gctgaattat aaacatgtgt
    21481 ataccctttt tctaaggttc catatggaga tatcaggtat acattatttc ttttagcagc
    21541 ttctgacatt gattcctcta tgtacttatt atgttctctt attaattcta atgttttaac
    21601 tccacctaaa gactctaaat tatatcctgt agcaaatagc tctggtaaac aaattatatc
    21661 tgccccttgt tttcctaagt catctatcat ctctactgct ttttctatat tttttttaac
    21721 atctccaagt acactgtgtt gttgaattat acctattttt accttacgac ccatgtaatt
    21781 gcccccttta tttatctttg attccaaaac atttcattaa tatataataa ataaccatac
    21841 tcactacaat tccatttatc ggaggtatta agaaatttat ttttgaacta taatacgacg
    21901 ctgcaaaacc tattaaccag cttattactg caatccaatt tattccttct ctatgcttat
    21961 aagttcttcc ttgtataaaa taatactcac accacataat tcctgctatt gcaggaactg
    22021 cgattcccaa gaaagataaa aatgactgaa atttaaatat tataccagta gctgctatta
    22081 ttgtacctat tagacctgca actaaagtca aaacatttct ttttacattt ggaaaaatag
    22141 tttttaatgc taatccacca gaataagcat ttgatacgtt tgtcgaccaa gatgcaagta
    22201 ttaaaataac aaacgctacc catccaaaac ctaaattagc caatacttct gttatgtccc
    22261 aagtaggtgc tcccatggac attacaccag ctgcaacttg ttggaataat gaaataatcc
    22321 ctagacctaa aaaactgata atccaaatat cttttactcg tctcgtatat cttaactggt
    22381 caggtgatat aattgcacca actataacaa gacctactac aatattaaga ccttcaacca
    22441 tactcatagt tgtttgagga gtataattat aaatagcttc aactccataa gtagaaaaag
    22501 ctttaaaaac tccccacacc attaatacca taagtggtgg tataactaag taattaaatt
    22561 tagccatagc tttaaagcca tacagtgcaa aaacaaccat aaataatcca agtataactg
    22621 taactaatgt taggtttaca cttgggaata tcttttgtac tgataatcct gcaacccctg
    22681 cttgtacacc aaaccatcca aaactagtta taccaagtaa tagagatata agatactttc
    22741 ctcctatctc tccaaatgcc cccttagcta atacatacgt atttagccct tctttgtaac
    22801 ctatataacc ttgtatcata ataattgcaa ctaatattaa gttccctata attgatgtaa
    22861 taatcatatt agaaagagtc attcctaaaa ttaaagttcc cccaaccatt agttcagata
    22921 tagctatgtt agaaccaagc atattaaaca atggagctaa ccatccaact ctttcctctt
    22981 ttggaactgg atttaaaact aattcttctc tctcttgcaa tattatccct ccctttcttt
    23041 ataaaagaat aatttctact tttttctact tttttttcac tattaataga atttttcttt
    23101 atataattgt aattctattc tataaaaaag tataatatct agtttgttat caatttgata
    23161 taatttaagt tataaataga taaatagtcc aaatttatta ttttaattta atctattaag
    23221 tcatatatta gacttatttc aaatttgtta tatattcttt aattaaaatc gtgtacttat
    23281 tacaaaagta gtttctaaaa caatcaaatt tatgtaaaat ttttaaacat agtattttaa
    23341 ttatattt
//
LOCUS       DAEPXF010000049        23340 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain HB27
            SAMN13376389-rid12479423.denovo.051, whole genome shotgun sequence.
ACCESSION   DAEPXF010000049 DAEPXF010000000
VERSION     DAEPXF010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13376389
            Sequence Read Archive: SRR10518432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 23340)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23340)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001069624.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001069624.1
            Genome Coverage       :: 315x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/15/2021 01:54:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,165
            CDSs (total)                      :: 4,076
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 89
            rRNAs                             :: 10, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 10 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 19 of 69
            Pseudo Genes (incomplete)         :: 44 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 10 of 69
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23340
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13376389-rid12479423.denovo.051"
                     /strain="HB27"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="China: Hebei"
                     /collection_date="2017"
     gene            204..905
                     /locus_tag="KRL67_001658"
     CDS             204..905
                     /locus_tag="KRL67_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5058 family protein"
                     /protein_id="HBH1522543.1"
                     /translation="MKFQEMINSPGLWIVSSFLVIISVVQAIVFMKAALKEASNLGIE
                     RKGIKAAIRSASVTAIGPSLSPVITLLSLVAVIGAPTTWMRLCDVGAARTELGVISLT
                     SNLSGVEVGSAAFGAEAFSYALWGMALNNLGWLFVVFILGHRMRGIVEKMNMKYNPTW
                     VKKLLTGATVGLFAYLLSNQIKTFEIPKLTPAIISAIVMLVLTTLFKKNQRLQELSLG
                     IAMLIGMFGTQIILS"
     gene            931..1587
                     /locus_tag="KRL67_001659"
     CDS             931..1587
                     /locus_tag="KRL67_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH1522544.1"
                     /translation="MYDKKMIKIGRISIGLAIIANFIPAIYVGIRFGQMPMLSVIFQI
                     WGLVAATYGVSWIVQPIAYYPTLGAPGSYIGWLAGSVGDIRMPAASMAQKIAGVEAGT
                     HEGEVVGTIGTCCSIFVSASMITIFTIVGYKVIPLLPEFVTNSFNYILPALFGAIYVD
                     IARKDIRAGGCTIAVALAIMYFGEKIGIPGGLLTLFIVLGGILITRVFFVIDNNKTKE
                     "
     gene            1897..3537
                     /locus_tag="KRL67_001660"
     CDS             1897..3537
                     /locus_tag="KRL67_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacylase"
                     /protein_id="HBH1522545.1"
                     /translation="MFDVKIVNGIIVDGTGNSRYKSDVGIVGDKIMAIGDLSQKEAKE
                     TIDATGKFVSPGFIDFHTHSDLSLVYDKYTRSRIHTGVTTDVIANCGIGVAPIREEKK
                     QELIDYLGTRIIGTIPTKLELHWNTMQEYFDYLTENSPAVNVVAYVAQGPVRINEMGF
                     SKEPATLEQLKNMKLEVRKAMEAGCVALSSGLVYLPGAYTKKEEMAELCKELIPYNGY
                     YISHIRDEGDEEMEALDEAIYIAKTAGVPLHISHLKVMGHKNFGTIDEVFKKLDEAEA
                     DGLEVTFDCYPYTAGMTSLGALLPPWAFEGGVENMVKRLEVQENRDRIIKELEEGIPG
                     WQCFYQLAGGWNGVVLASVMTEANKYVEGKTLMEVAKINGENPFDTFFRLLIEEKSKI
                     QVVVHTQGQEDTDKVVCHPKSCIGSDSMDLSTEGLLSLGKPHPRAFGTFGRIFSYYVR
                     EKGMLTFEEAVKKITYLSAKRLGIYKERGLLKENYFADIVVFDPDTIEDKATYSNPKQ
                     YTVGVEYVLVNGKIALAEGKQTDVCAGRVIKNPLSIAK"
     gene            3894..5216
                     /locus_tag="KRL67_001661"
     CDS             3894..5216
                     /locus_tag="KRL67_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH1522546.1"
                     /translation="MKILYVGPGMVIDKILAVSKKNFPNIEADFLRYDRYIEVPDLLE
                     KYRGRVDAVLFTGKSPFKFFEKRQKDFVLSDYIPRHETTLYRVLLEITYLLKFDISRL
                     SIDTYDKAMMKRLYKEIGVEFKDEHMFFAEQKYLEEDYIKFVLEFHKYNYQNNDICCC
                     ITGLEEAYHIMKKDNIPVVLALPSEDVMVQSIKNIQMRYIAKKNNENQIVVLAIKLNM
                     PSEYSLMKEDEYAYLSQRIKILDKLYHFNSRIDGVLVEQSRSEFMIFTTKKILELETK
                     KYRDIYLLDMIREVSVINTYIGIGYGKTANEAKFNAYESIKMAQSQKNDAAYVVFENG
                     EIMGPLKPNPTVKEKNSFDERFYQIATETGLSANTVYKVFNKIINSGKTEFTSKELAT
                     ICGVSIRTMDRIILRLCDSGYCEVVSEQLMSKYGRPSRILRFKPFLLF"
     gene            5413..5715
                     /locus_tag="KRL67_001662"
                     /pseudo
     CDS             5413..5715
                     /locus_tag="KRL67_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895717.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
     gene            6360..7478
                     /locus_tag="KRL67_001663"
     CDS             6360..7478
                     /locus_tag="KRL67_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBH1522547.1"
                     /translation="MIVVEKAYKFRMYPNKKQQELINKTFGCCRFVYNKYLAKRIEVY
                     KNDKETFTYKQCSSDLTNLKKELKWLKEPDKFSLQNALKDLDNAYKKFFKEKVGFPKF
                     KSKKINRFSYKTNFTNGNIMYLGQHIKLPKLGMIKIRDKQVPQGRILNATISKEPSGR
                     YYVSLCCTDVDIEAFENTNNQIGLDLGIKEFCISSCGEFIENPKYLKKSLNKLAKLQR
                     ELSRKTIGSLNRNKARLKVARLQEHIANQRKDFLQKLSTKLIKENDIICIEDLQVKNM
                     IRNRKLSRLISDVSWSEFIRQLEYKANWHGRQIVKVGKFFASSQICNKCGYKNEEVKN
                     LNIREWICPSCNETHNRDINASINILKEGLRLITIQNK"
     gene            7596..8300
                     /locus_tag="KRL67_001664"
                     /pseudo
     CDS             7596..8300
                     /locus_tag="KRL67_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895717.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
     gene            8338..9750
                     /locus_tag="KRL67_001665"
     CDS             8338..9750
                     /locus_tag="KRL67_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ester cyclase"
                     /protein_id="HBH1522548.1"
                     /translation="MASTNKNNVTSKGEEKEASTSGLESCTVKENKAKTNQTNFSNIN
                     ENQTNENKTNTYKGDIDKGNIDKTNTNKSDINKVNIDTMDTDKLNTNTLTEKNKKVIF
                     KGEDNMSSTKIYYGDSLEVTPVGVMDYNDFSKQTKREQEIPGFDSKYRDFVDYIMKIT
                     HNIWEEKGIGVIYDTYHNNVTMHCGSSNLVGIKDVISNTLQTLHAFPDRRLIGQNVIW
                     SNFGADGFLSSHRVLSTATNLGDSNFGPATGRKINFRTVIDCATTNNRIHEEWLVRDN
                     LWIVTQLGLNPQEVAKSMAKASESKVLSLQSTYGICESMDGQFMPTKYQAKDDSVGEM
                     MLEMTSRIYNYKYINEVKKYYHDNAVVHFICDKDLNGYDEIQGMIVSLLASIPNGSYE
                     VERVTCNQREKNEGYDVSVRWRLRGINEGIGFFGQPSGKHIEVMGINHYHILQGKVKE
                     EWITFDGMDVLKQMYMGVEE"
     gene            9895..11160
                     /locus_tag="KRL67_001666"
     CDS             9895..11160
                     /locus_tag="KRL67_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBH1522549.1"
                     /translation="MKTTVEIENDIILGKDYSNLKRGIAFFSIALIYFFYCYNFMVGT
                     FIKPTMIYALADGGFGFSLRQTEEIFAVMSFGTIPGTFIFGVISTKIGKKRTLISVAL
                     LIGLTTFIPMLSPTNIMLWKIARLCTGVVLGGVFGTAMPLVADMFPSKYRGKLAAVLT
                     ALFSLAMIFGGKVYGVLGDANWQILMYTAIIPPVVGAILAILFVPDDLEYTKELVKKG
                     KETGEKISYISMYKGKYLWIGLGTILLSGANFTAYSAFSNNATTYLVTGIGMSAAVAG
                     SIYSLQGIGQLVGYLFWGSIADKFGRKIPFIGMALAAVFVFIYTKLGGDNTTTFYMIS
                     VLLGVAVGYSGAWGAYYTELFPSKYRSLSSGMSFNGGRIISTFAIPAIAGTASGTLGM
                     MTIFYISIAVFVAGAIVWLFLPETLNNKK"
     gene            11202..12800
                     /locus_tag="KRL67_001667"
     CDS             11202..12800
                     /locus_tag="KRL67_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter"
                     /protein_id="HBH1522550.1"
                     /translation="MISSIDVCIIIGYLLLMLVIGYYSGKDNKNQEDYFLAGRSMPWI
                     PIALSVAATMISANGFIGGPGWAYVDGMYPVMVNITVPLAIFFALSITTPVIYKLKIT
                     SIYEYMGLRLGNYSRGLTIIQFFINSLIQASSIVYIPVLIIQMITGWSLYALVPIVVL
                     VSIIYTLLGGIKAVIWTDSIQMIVVISAVFIVIIVALKGMNLSFFDTLQIAQQSGKLN
                     TFDFSRDISVTNTFWATLIGGTVMWIRYFCFDQSQVQRVLTSKSLKCAKNSFVVSAFV
                     MNLVYYFMLLVGVILFVFYGGRTFETSNEIMITFILNELPVGAIGIIIAGVFAAAMSS
                     IDSILNSMTTVFTKDIYEFYFKKDNKESSLKVTMIITVVIGVVMTGFIIMGFGGSIKS
                     ILDLVGNYISYFAGPATGAFVLAMFTYKANDKGVSIGFIVGLVLGFFISNKYNVSWLW
                     NPAIGATITLIFGYIFSVILKKNHNVDDIKKYTAFGMRNHLISQGMDKVDGVSIVPFS
                     LGKQELIVLAFFIIQYIILFLIQF"
     gene            12835..13857
                     /locus_tag="KRL67_001668"
     CDS             12835..13857
                     /locus_tag="KRL67_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBH1522551.1"
                     /translation="MNQKKVHYNKSATSKDVARLAGVSQSSVSRAFGSANGKGVKPEV
                     RERIFRVANEIGYVPNLVARGMISGKTNVIGLIVGDSLGPFYNRIINLFVEKIQEIGK
                     QCLVFKVPRQQSIDSIISKVIQFQVEAVIITASAMNKVMAETCEENNIPVILFNRFIP
                     GIKISTVYVDPIEGGGMAAEYLLKKGHKNIGYIQFTRETSEEMEKKIGFYSKLRQSGI
                     HNLKEESANYDYDSGYEAGKRMLALSNPPTAIFCTSDLIAIGVMDAARFEYKLRVPED
                     LSVIGYDDIQMASWKSYNLTTIRQPLDLLIEKTINILKSLLNEENPESVVEMLKPELI
                     ERGSTL"
     gene            complement(14264..15667)
                     /locus_tag="KRL67_001669"
     CDS             complement(14264..15667)
                     /locus_tag="KRL67_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBH1522552.1"
                     /translation="MKMLNNFFKKINNPAPVFAQTKMERDNQTDDSLSTKPKTTFRDV
                     AGLDEVKEELFEIVDFMKSPQKYQKMGAKIPKGVLFYGPPGTGKTLLASAVAGETNSS
                     FFNVTGSEFVEKYVGVGAKRVRTLFEKARKEAPSIIFIDEIDAVGAKRHLESNNEKDQ
                     TLNQLLVEMDGFNKDSNVLIIGATNRLDLLDEALLRPGRFDRHIHIGAPNYHTRFEIL
                     KVHTDDKPIDKSVNLELLAKKTHGFNGAHLSNIANEAAIFAVRDDSECITSEHFDKAL
                     ERVIAGLESKNSALVEKEKKIVAYHEAGHALVSDIVGICPIQKISIVPRGQALGYVLQ
                     LPDEDRYIYTKDELIGKIKILLAGKASEELIFNHKSTGAKDDLKKVTEIANQMVCEYG
                     MSNLGFMTIDGNDKTFLCDKVQKEANRIVEVCYKETLEMLKDNLEDLHSVSKFLFEKE
                     TMTHEELKDLIGKEAVN"
     gene            16655..17164
                     /locus_tag="KRL67_001670"
     CDS             16655..17164
                     /locus_tag="KRL67_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009528606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HBH1522553.1"
                     /translation="MSKELAINEQIKDKEIRVLSPTGEQLGVMPTKEAQAMANSKNLD
                     LVQISPNANPPVCKIMDYGKFRYEQARKEKEAKKKQKTIVVKEVRLRPGIEQNDLNTK
                     ANNAIKFLKKGDKVKVELRFRGRELGHKDIGKEVMLKFLDIIKEFGEPTKAPAFEGNN
                     MVVIIDPKK"
     gene            17192..17386
                     /gene="rpmI"
                     /locus_tag="KRL67_001671"
     CDS             17192..17386
                     /gene="rpmI"
                     /locus_tag="KRL67_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011721840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HBH1522554.1"
                     /translation="MPKMKTHRGAAKRLKKTGTGKLKRAKAFKKHILTKKSAKTKMNL
                     RKSTLVSDGDAKRIAQLLPY"
     gene            17441..17797
                     /gene="rplT"
                     /locus_tag="KRL67_001672"
     CDS             17441..17797
                     /gene="rplT"
                     /locus_tag="KRL67_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HBH1522555.1"
                     /translation="MARVKKAMNARKKHKKILKLAKGFRGSRSKLYRPANTFVMKALK
                     NAYIGRKLKKRDFRKLWIQRINAAARMNGISYSRLMNGLKLSGVEVNRKMLSEMAIQD
                     PEGFAKLAEVAKAKLA"
     gene            18107..18652
                     /locus_tag="KRL67_001673"
     CDS             18107..18652
                     /locus_tag="KRL67_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH1522556.1"
                     /translation="MNEKLMDCFVNPIKCKLLLEIFSNNRTTAKQLGEKFQDISKATL
                     YRYLNKMLKDDIIRVVEENKIRGTVEKVYAMKLDIADDVNKIIDENSGEEYAQLFTQY
                     MMGFLREFYEYAERKEKDILKDGSGFSVCPVYASIDEIVEVSKKIASILEPLRNNSHE
                     EGRRMHSIGMIITPPKEDEGK"
     gene            19089..21014
                     /locus_tag="KRL67_001674"
     CDS             19089..21014
                     /locus_tag="KRL67_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HBH1522557.1"
                     /translation="MKIKFVIMPLVIFLMVLFVGCQSTKDTEVKAKDTEIKILGTGDL
                     HSILTDSIVSYVNEEREKNKNLLMVDAGDFYGTQSREMWEWSSGKKLINIRRNGRAEY
                     DDIIKNSKDEVPIVKDMAKLKYDAVVLGDNEFVSNDKQSLDKLVSDFKNNNMPLVSAN
                     IYEQSGENYVQPYVMKNIKTDGGNVKVGILGLTIKEVGETLGLEDFEKDKKARELGEQ
                     ADYKGKLYANDLVEDAKKWIKVMEKESPDIIVAVVHSGEEPKTDRNPGNRIKELATTV
                     DGIDAIVAGHTHEKIDEHKYKNKSGDEVIVTQPGWHGDRVSEVSFKLNKENGKWDIKE
                     KSSKLKIIDKEVSIVATADLHSHFSDKLTVDLVNERSNPIEPVVVDAGDFLDPQTDEM
                     TQWYKEWKNIRDNNSGKTISRCPMVMDMNQGMYDAVVLGNHEFVSEKDEFWSDERLLD
                     AVIEDFEQVAISVLSANIYRQSGKNYVKPYIIKDVETSEGNVKVGILGLTIKEVGKGL
                     ENVNLQEQFQYKGKLYANDLVEDAKKWVKVMKKKKPDIIVAVVHSGEEPKNPKHPGNR
                     IKELATTVEGIDAIVASHTHEKIDEHEYKNKSGEKVIVTQPGEHGKYYSKITFKVNKE
                     KDSWIINDKFSKTVEVE"
     gene            21409..23313
                     /locus_tag="KRL67_001675"
     CDS             21409..23313
                     /locus_tag="KRL67_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HBH1522558.1"
                     /translation="MKIKFIIVASIIFLIVLLVGCQSTKDTEINVLATSDLHSIIPDN
                     LISYVEEERKYDENLLMVDAGDFFDMQSGEMNKWFTGQKLVRFKDGIPEYKKIAEPRE
                     GEVPLAKKMAKLKYDAVTFGNHEFVSNDKQSLDKLVSDFKNNNIPLVSANVYEQSGEN
                     YVKPYVMKNINTDKGSVKVGILGLTIKEVGERSRFKSFEQDKKARELGEQPQYKGKLY
                     ANDLVEDANKWVKVMEKKEKPDIIVAIVHSGEEPKNPKNPGNRIKELATTVEGIDAIV
                     AGHTHEKIEQHTYKNNSGEEVIVTQPGAHGNSISKINFKLDKKNDKWFIKDKYSELKV
                     FGKEVNIITTSGLNPKFSDEMAVNLFNERDRDVQPIFVDTGDFLNTNTKEMTKWAKEW
                     QYNENKGIYKKINEWPIVYLGYDAVVLGSHEFTEDKETFEEKKSTLDYIIENFEEMKI
                     PVLSANIYDESGENYVKPYIIHNVETREGNIKVGVLGLTTTDKDEEDLSSSSLSKDSK
                     TSKIPEETGKLYTNNLVKDASEWVKVMQKESPDVIVAVVHSDNNSTLKNPSSEVKKLA
                     MSVDGIDAIVVGHTKNKVEEHIYKNKSGDEVIVTQSGEDCYSKISLELRKENGKWNII
                     NKYSKSIKMK"
BASE COUNT         8847 a         2616 c         4407 g         7470 t
ORIGIN      
        1 taatatgtga attttctgat atttaccaat tatcaataaa atatttaagt attaatgttt
       61 ttagtgagac tttaagacag ataatctcct attaaagata ctatatctta gataaaataa
      121 attaaaacaa tataaaaaca taacaagatt acagaaagct cacaataaaa cattaaaaaa
      181 tttattttaa ggaggaataa agtatgaagt ttcaagaaat gattaatagc ccaggtcttt
      241 ggattgtttc tagtttttta gtaattattt cagttgttca agcaatcgta tttatgaaag
      301 cagcgctaaa agaggcttca aatcttggta ttgaaaggaa aggaattaaa gcagcaattc
      361 gctcagctag tgttacagca attggacctt ctctatctcc tgttattaca ttattatcac
      421 tggtagctgt gattggagct cctacgactt ggatgaggct ctgtgatgtc ggagctgcta
      481 gaacagagct tggagtaata tctcttacat ccaatttatc tggggtagaa gtaggtagtg
      541 ctgcatttgg agcagaagct ttttcatatg cactatgggg aatggcttta aataacttag
      601 gttggctgtt tgttgtattt attttgggtc atagaatgcg tggaattgtt gaaaagatga
      661 acatgaagta taatcctact tgggttaaaa aactacttac aggagctaca gttggattat
      721 ttgcttacct tctttcaaat caaataaaaa catttgaaat accaaaactt acaccagcaa
      781 ttatttcagc tatagttatg ctagtattga caactttatt taaaaagaat caacgattac
      841 aagaattatc cttaggtatt gccatgttaa ttggtatgtt tggtacacag attatattat
      901 cgtgattaaa acggataaga ggtgataatt atgtatgata aaaagatgat aaaaataggg
      961 agaatttcta taggtcttgc aattattgca aactttattc ccgctatcta tgtaggtatt
     1021 cgctttggtc agatgccaat gctttctgtt atatttcaaa tttgggggtt agtcgctgcg
     1081 acttatgggg ttagttggat tgttcaacct atagcttatt atccgacact aggagctcca
     1141 ggttcttata taggttggct tgcaggttct gttggagata tacgtatgcc tgcagcatca
     1201 atggcacaga aaattgcagg tgtagaagca ggaactcatg agggtgaagt tgttggaaca
     1261 attggtacat gttgctcaat ctttgtatca gcttctatga ttaccatttt taccattgta
     1321 ggctataaag taattccttt gttacctgaa tttgttacta attcatttaa ttatatactt
     1381 ccagcattat ttggagcaat ttatgttgat attgcacgta aggatattcg agctggtgga
     1441 tgtacgattg ctgtagcgtt agcaattatg tactttggtg aaaaaattgg tataccaggt
     1501 ggattattaa cattgtttat tgtgttaggt gggatactta tcacaagggt cttctttgta
     1561 attgataaca ataaaacaaa agaataggat tgcttattaa ggaggaagac tagaattatt
     1621 tgttgagaaa aaagaatagg attgtttatt aaggagaaag actagaatta tttgttaaga
     1681 aaaagaatag gattgtttat taaggagaaa gactagaata atttgttgag aaaaggaaca
     1741 ggattgttta ttaaggagaa agactagaat tatttgttaa gaaaaggaac aggattgttt
     1801 attaaggaga aagactagaa ttatttgttg agaaaaaaga ataggattgt ttattaagaa
     1861 atatgaatat aaataagtct tgaaaggaga ataagcatgt ttgatgttaa aattgtcaat
     1921 ggaataatcg ttgatggtac aggaaattca agatataaaa gtgatgtagg gattgtagga
     1981 gacaaaatca tggctattgg agatttaagt caaaaagagg ctaaagagac tatagacgca
     2041 actggaaaat ttgtatcacc tggttttata gatttccata ctcattctga tttatctctt
     2101 gtgtatgata aatacacaag aagccgtatc catacaggtg taacaacaga tgttattgca
     2161 aactgtggta taggagttgc tccgataaga gaagagaaga agcaagagtt aattgactat
     2221 cttggaacta ggataattgg tacaattcca acaaaattag agcttcattg gaatacaatg
     2281 caagaatact ttgattactt aactgaaaat tctccagcag tcaatgttgt agcttatgta
     2341 gcacaaggac ctgtacgtat caatgaaatg ggattttcaa aggaaccagc tactttagag
     2401 caacttaaaa atatgaagtt ggaagttaga aaagctatgg aagctggatg tgtagctctt
     2461 tcatcaggat tagtttattt accaggagca tacacaaaga aagaagaaat ggctgaatta
     2521 tgtaaggaat tgataccata caatggatac tatatatctc atattagaga tgaaggtgat
     2581 gaagaaatgg aagctcttga tgaagcaatt tatatagcta agactgcagg agttcctcta
     2641 catatttctc atttaaaagt catgggtcat aagaattttg gaactattga tgaggtattt
     2701 aaaaagctag atgaagctga ggcagatggt ttggaagtaa cttttgattg ttacccatat
     2761 acagctggaa tgacatcttt gggtgcactt ttacctcctt gggcttttga aggcggcgta
     2821 gaaaacatgg taaaacgatt agaagtacaa gaaaatagag acagaattat aaaagagttg
     2881 gaggaaggga ttcctggatg gcaatgtttc taccaattag caggtgggtg gaacggtgtt
     2941 gtactagcct ctgtaatgac tgaagcaaat aaatatgtag agggtaaaac tttaatggag
     3001 gtagccaaaa tcaatggtga aaatccattt gatacattct ttaggctttt gattgaagaa
     3061 aaaagtaaaa ttcaagtagt tgttcataca caaggtcaag aggatacaga taaggtcgtt
     3121 tgccatccaa agtcatgtat aggttctgat agtatggacc tttctacaga aggtttactt
     3181 tctcttggaa agcctcatcc tcgtgcattt ggtacttttg gacgaatttt ctcttactat
     3241 gttagagaaa aaggtatgtt gacttttgag gaagcagtta aaaagataac ttatctttca
     3301 gcaaaacgct taggcattta taaagaaaga ggtctactta aggaaaatta ttttgctgac
     3361 attgtggtgt ttgacccaga tacaatagaa gataaagcga cttatagtaa tcctaagcag
     3421 tatacagtgg gagtggaata tgtgttggtg aatggtaaaa ttgcgttggc agaaggaaag
     3481 caaactgatg tatgtgctgg acgtgtaatt aaaaatcctc ttagcatagc aaaatagaat
     3541 acattaagaa cttttataaa aatagtcttt ctttaaatat aaggaaggct atttttgtta
     3601 gattaataaa gattgttata attgtgtttt ctaaagaatt taactttaag tagaaaaaac
     3661 aatatattaa ttgtaaaatg taattaatat ttatgaataa gatttaaata caaaaattta
     3721 aatacaaaaa tgtttcaaca gatagtcgta attaaaaaac ttaaagtaac tttttatatt
     3781 aggtataatt gattattcaa aattctatga atcaggagtc ctgtgacttt agtcatggta
     3841 ggtttagaaa atgttttaag agataatagt atcattagga aaggagagac gaaatgaaaa
     3901 tactttatgt aggaccaggt atggtaatcg ataaaatact agcagtaagt aaaaagaatt
     3961 ttcctaatat tgaagcagat tttcttagat atgatagata tattgaagta cctgatttat
     4021 tggaaaaata tagaggtaga gtggatgcag ttctttttac aggtaagtcg ccatttaaat
     4081 tttttgaaaa aagacaaaaa gattttgtat tgtctgatta tattcctaga catgaaacaa
     4141 cattgtacag agtgttgctt gaaattacat atttgcttaa atttgatatt agtagactta
     4201 gtatagatac ttatgataag gctatgatga agagattgta taaagaaatt ggagttgaat
     4261 ttaaggatga acatatgttt tttgcagagc aaaagtattt agaagaggac tatataaagt
     4321 ttgtgcttga atttcataaa tataattatc aaaataatga tatatgttgt tgtattacag
     4381 gtcttgaaga ggcctatcat ataatgaaaa aagataatat tccagtagtt ttagccttgc
     4441 cttctgagga cgtaatggtg caaagtataa aaaacattca gatgcgttat attgcaaaga
     4501 aaaacaatga gaatcagatt gttgttcttg ctattaaact gaatatgcct tcagaatatt
     4561 cactgatgaa ggaagatgag tacgcttatc tttcacaaag gattaagatt cttgataaac
     4621 tataccattt caatagccgt atagatggtg ttttggtaga gcagagtaga agtgaattta
     4681 tgatttttac aactaaaaaa atacttgagt tagaaacaaa gaaatacaga gatatttatt
     4741 tgttggatat gattcgtgaa gtttcagtta taaacactta tataggaatt ggttatggaa
     4801 aaacagcaaa tgaagctaaa tttaacgctt atgagagtat taagatggct caaagtcaaa
     4861 aaaatgatgc agcatatgta gtttttgaaa atggagaaat tatgggacca ctaaagccaa
     4921 atcctactgt caaagagaaa aatagttttg atgaacgttt ttatcaaatt gctacagaga
     4981 cagggctaag tgctaatact gtatataaag tatttaataa aataataaat agtggaaaaa
     5041 ctgaatttac atcaaaggaa ttagctacta tatgtggtgt aagtattaga actatggaca
     5101 ggattatact tagattgtgt gattctggtt attgtgaggt agtgagcgaa cagcttatga
     5161 gcaagtatgg tagacccagt agaatactgc gttttaaacc ttttttgttg ttttaaatag
     5221 ctataagtca aaaattaaat ttttatgata acattttctt tacaattttt tagatatatg
     5281 ttaatattta gaaaaatgca tatgtattca taaaataaac ttaaaacagc taaaaaatca
     5341 ttaaaaggga aaacgtatgc ataaaataag aaagttaatt tataacttaa ataaatctgg
     5401 gaggagatag ttatgggaaa atatcaggat tcaaaaaaag ttgtaagaaa ttattttgaa
     5461 gcattagaaa atgctactgg agatgaggtt gaaaatgtct taaagcaaca tatgaaatca
     5521 gattataact ggaaaggtgt gtatccattt cgtgaacaag aagggactga aaatgtagca
     5581 gatgtatttt ggaggccttt aaaaaaatca ttgagtaata tgcaaagacg acaagatatt
     5641 ttcattgcag gaacaaatga gattgataat agtgaatggg taatgagtat ggggcatttt
     5701 atgggacttt ttgattaaaa aatagctacg cacactcgtg attttagtca tgagttagta
     5761 gctaaaatag tcagcatata gggaaaccta tatgtagaga taggatagaa cctatccaat
     5821 actacttgaa ttgctggaaa cccctaaagc taatcaaact aaaacataag gatgaaataa
     5881 acctaagtgt gaaagttacg aaagtagaaa aaattgatta gatggtataa ggttaaaccc
     5941 taagtacttt aaaatgggca atcagcaggt aaactccgaa taggagaaac ttcaacgact
     6001 attcctcttg agggaagtac actacaagtt atcggtagtg gaagtgggta gaccttaacg
     6061 gataatgccg aaggataaga tatagtctgt gcttgcatga aagtgtaaga agttcataag
     6121 agaactgcat aggtagtagc gaacttatgt gaacgacaac ctcaaaaatg ataaaagtta
     6181 gtacttacgg ttcttccata tgttatttaa aaataaatat ataaaccttc gaaataattg
     6241 ttttaaataa ttacgtttgt taatattata atgagtgatg aaaaaaggaa gttactcatg
     6301 gtagagaaca tgtatactaa taatcgtata ttaataatca gaaaaacagt taaagtgagg
     6361 tgatagtagt ggaaaaagct tataagttta gaatgtatcc aaataaaaaa caacaagagt
     6421 taattaacaa aacttttgga tgttgtagat ttgtatataa taagtatctt gctaaaagaa
     6481 tagaagttta taaaaacgat aaagaaactt ttacttataa acagtgtagt tctgatttga
     6541 ccaacttaaa aaaagaatta aagtggctta aagaacctga caaattttca ctacaaaacg
     6601 ccttaaaaga tttagataat gcatataaaa aattctttaa agaaaaagta ggatttccta
     6661 aatttaaatc aaagaaaatt aatagatttt catataaaac taactttaca aatggaaaca
     6721 ttatgtacct tggtcaacat ataaaactac ctaaacttgg tatgattaag ataagggata
     6781 aacaagtacc tcaaggtaga atacttaatg ccactatatc taaagaacca agtggtagat
     6841 actatgtatc attatgctgt actgatgtag atattgaagc atttgaaaat actaataatc
     6901 agataggttt agatttggga attaaagaat tttgtatctc aagttgtgga gaatttatag
     6961 aaaatcctaa gtatcttaag aagtcattaa acaaacttgc taaattacaa agagaattat
     7021 cgagaaaaac aatcggtagt ttaaatagaa ataaagcaag attaaaagtt gcaagacttc
     7081 aagaacatat agctaatcaa agaaaggatt ttctacaaaa actatctact aaattaataa
     7141 aagagaatga tattatttgc atagaagatt tacaagtaaa gaatatgatt agaaatcgta
     7201 aactatctcg cttaatttct gatgtgtcat ggtcagagtt tattcgtcaa ttagaataca
     7261 aggctaattg gcatggtaga caaattgtaa aggtgggtaa attctttgca agttcacaaa
     7321 tatgcaataa atgtgggtac aaaaacgagg aagtgaagaa cttaaatata agagaatgga
     7381 tttgtcctag ttgtaatgaa actcacaata gagatataaa tgcaagtata aatatattaa
     7441 aagaaggatt aagattaata acaattcaaa ataaataact tatgtaagaa ccgtaggaac
     7501 tacggggata gcctgtggag aattagtaag acatatttta gtatgcaaaa ttctatgaag
     7561 caggaaccct gtgactttag tcatgggagg ttcagaagga ttggcttggt attaaacata
     7621 ctcgtaaaat gattagttta aggtatgcag aatttaactg tgttcaagat ggtaaaattg
     7681 ctaaaactgg attatttgta gatataatcg ggtttatgat tcaagcagga gttaatccat
     7741 tgccaccaca aacaggttca tattttgtat atccaggccc aattgaccat aatggattac
     7801 tttttgaaga tgcagatgaa gttgaaggag taaaaacttt agcattagta aataaaatgg
     7861 tagatgattt atctgaatta aatgaaagtg gggctatggg atgtccacca gaaatacttg
     7921 aaaaatcttg ggctaaagat atgatttggt atggtccagg tggaattggt gcttcttata
     7981 ctatacctag atatcaagaa caacatcagc taccatttag aaataattta aaaggaaaga
     8041 cttttaatgg gcatgtatgt agatttgcag aaggtaattt tgcttgtttc tttggatggc
     8101 ctaatctgac taatgtacca tgtggaggtt ttttaggatt accaggtgga gaggttaaag
     8161 cagatatgca agttgttgat gtttattatc gtaaaggaga taaacttcaa gaaaactggg
     8221 tgttaattga tattccttat tggttgaaac aacaagggct tgatattctt gagagaacta
     8281 aaagtatttt taatgcttaa ttatgcatac gtattctaaa acgaaggggg atataaagtg
     8341 gcatctacca acaaaaataa tgtaacaagt aaaggggaag aaaaagaagc ttctacaagt
     8401 gggcttgaat catgtacggt taaagaaaac aaagctaaaa caaatcaaac taattttagt
     8461 aatataaatg aaaatcaaac aaatgagaat aaaacgaata catataaagg agatatagat
     8521 aaagggaata tagataaaac caacacaaat aaatccgaca taaataaagt taacatagat
     8581 acaatggata cagataaatt aaatacaaat actcttactg aaaaaaataa aaaagttata
     8641 tttaaggggg aagataatat gtcatctaca aaaatttact atggagattc attagaagta
     8701 acgcctgttg gagtaatgga ttacaatgat ttttcaaagc agacaaaaag ggagcaggag
     8761 ataccaggat ttgattctaa gtatagagat tttgtagatt atattatgaa aattacacat
     8821 aatatctggg aagaaaaagg tataggtgtt atttatgata catatcacaa taatgttaca
     8881 atgcattgtg gttcttcaaa tttagttggt attaaagatg ttatttccaa cactcttcaa
     8941 acattgcatg cgtttcctga tagaagatta attggtcaga atgtaatatg gtctaacttt
     9001 ggtgctgatg gttttttatc ttctcaccgt gttttgtcta cagcaactaa cttaggtgat
     9061 agtaattttg gaccagcaac aggtagaaaa ataaacttta gaactgtaat tgattgtgcc
     9121 acaactaaca atagaatcca cgaagaatgg ttggttagag ataatttatg gattgttact
     9181 caattaggat taaaccctca ggaagtagca aagagtatgg caaaggcaag tgaatctaaa
     9241 gtgcttagtt tacaatcaac ttatggtatc tgtgaatcta tggatggtca atttatgcca
     9301 actaagtatc aagcaaaaga tgattctgtt ggcgaaatga tgcttgaaat gacaagtcgt
     9361 atttataatt ataaatatat aaatgaagtt aaaaaatact atcatgataa tgcagtagtc
     9421 cattttatat gtgataaaga tttaaatggt tatgatgaga ttcaaggaat gattgtaagc
     9481 ttacttgcat caattccaaa tggaagttat gaagtggaaa gagtaacttg taatcaaaga
     9541 gaaaaaaatg aaggttatga tgtatctgta agatggagat tacgtggaat aaatgaaggt
     9601 ataggatttt ttggacagcc ttctggaaag catatagagg taatgggaat caatcattat
     9661 catatactac aaggtaaagt aaaggaggag tggataactt ttgatggtat ggatgtctta
     9721 aaacaaatgt atatgggagt agaagaataa ggctgtttag agtataaata gttttcaaat
     9781 gtatataggg aagtattgtt ttgtaaaatt taagtaggga atgtatatga aaaatagatg
     9841 tttaaacttt tagcaaaatt atgactaata tttaaaaggg ggaattataa aaatatgaaa
     9901 acaacggttg aaatagaaaa tgatatcata ttaggaaaag actattcaaa cttaaaaaga
     9961 ggaattgcat ttttttcaat tgctttaata tacttttttt attgctacaa ttttatggta
    10021 ggtactttta ttaaaccaac aatgatttat gctttagctg atgggggatt tggtttttca
    10081 ttaagacaaa cagaagaaat atttgcagtt atgtcttttg gtacaattcc aggtacgttt
    10141 atctttgggg tgatttctac aaaaattggg aagaagagga ctttaattag tgttgcctta
    10201 ctgattggat tgacaacatt tataccaatg ttatctccta caaatattat gttatggaaa
    10261 atagcacgtt tgtgtacagg agtagtgtta gggggcgtgt ttggaacagc tatgccttta
    10321 gttgcagata tgtttccaag taaatatcgt ggtaagttag ctgctgtatt aactgcttta
    10381 ttttcattag ccatgatttt tggtgggaaa gtatatggcg ttttaggtga tgcaaattgg
    10441 caaatactga tgtatactgc gattatacca cctgtagttg gagctatctt agcaatttta
    10501 tttgttccag atgatttgga gtatacaaag gaattagtta aaaagggaaa agaaactggt
    10561 gaaaagatta gctatataag tatgtataag ggaaaatact tatggattgg attgggaacg
    10621 attcttttat caggtgcaaa ttttacagca tattcagcat tttcaaataa tgcaactaca
    10681 tacctagtga caggaatagg aatgtcagca gcagtggctg gttctatata tagtttacag
    10741 ggaattggtc aattagtagg atatttattc tggggttcaa ttgcagataa atttggaaga
    10801 aaaatacctt ttataggtat ggcattagca gcagtatttg tttttatata tacaaaatta
    10861 ggtggagata atacaacaac attctacatg atatcagtat tactaggtgt tgctgttgga
    10921 tattctggag catggggagc atactacaca gagttgttcc caagtaaata tcgttcgttg
    10981 tcatctggta tgtcatttaa tggaggaagg attatatcta catttgcaat acctgctata
    11041 gctggtacag cttctggtac tttaggtatg atgactatct tttacatatc aatagcagtc
    11101 tttgtagcag gtgctattgt gtggttattc ttaccagaga cgttaaataa taagaaataa
    11161 aatactagga atttattttg ttacttagaa aggggtggct tatgatttca tcaattgatg
    11221 tatgtattat aataggatat ttgctattaa tgttagttat tggttattat tcaggaaaag
    11281 ataataaaaa tcaagaagat tatttcttgg caggtaggtc aatgccatgg attccaattg
    11341 ctttatcagt tgcagctact atgattagtg caaatggttt tataggtggt cctggatggg
    11401 catatgtaga tggaatgtat ccagtaatgg ttaatattac agtaccttta gcgatatttt
    11461 ttgcactgag cattaccacc ccagttatct ataaattaaa aataacctct atatatgaat
    11521 atatgggact tcgtttagga aattattcga gaggattaac tattattcaa ttttttatta
    11581 attcattgat tcaagctagt tctatagtat atattccagt gcttattatt caaatgatta
    11641 ctggctggtc actttatgct ttagtcccaa tagttgtact tgtatcaatt atatatactc
    11701 tattaggtgg gattaaggca gttatatgga ctgatagcat tcaaatgata gtagtaataa
    11761 gtgctgtatt tatagtaatt attgtagcct taaaaggaat gaatctaagt ttctttgata
    11821 cattacaaat tgcacaacag agtggaaaat taaatacatt tgatttcagt agggatattt
    11881 cagttacaaa tactttttgg gctactttaa ttggtggaac tgtaatgtgg ataagatact
    11941 tctgttttga ccaatctcaa gtacaaagag ttttaacatc taaatcttta aaatgtgcaa
    12001 aaaattcttt tgtagttagt gcatttgtta tgaatttagt ttactacttt atgctattgg
    12061 taggagtaat tttatttgtt ttttacgggg gaaggacatt tgaaacttca aatgaaataa
    12121 tgattacttt tattttaaat gaacttccag taggagccat tggaattatt attgcaggtg
    12181 tgtttgcagc agcaatgtct agtatcgact ctatattaaa cagtatgaca actgttttta
    12241 cgaaggatat ttatgagttt tatttcaaaa aagataacaa agaatcatca ttaaaagtta
    12301 caatgattat aactgtagtt ataggagttg ttatgacagg ttttattatt atgggatttg
    12361 gaggaagcat taaatcaatt cttgaccttg ttggtaacta tatctcatac tttgctggac
    12421 cagctacagg tgcatttgta ctagcaatgt ttacatataa agctaatgat aaaggagtat
    12481 ctattggttt tattgtaggg ttagttttag gattttttat ttctaataaa tataatgtga
    12541 gttggttgtg gaatccagca ataggtgcta caataacatt gatatttgga tatattttta
    12601 gtgtcatttt aaagaaaaat cataatgtag atgatattaa aaaatacact gcatttggta
    12661 tgagaaatca ccttatttct caaggaatgg ataaagtaga tggagtatct atagtgccat
    12721 tttctttagg taaacaagaa ttaattgtat tagctttttt tattatccaa tatattatat
    12781 tatttttgat acaattttaa gaagaaatac tagatattta aggagaaata ctaaatgaat
    12841 caaaagaaag ttcattataa taaatcggct acatcaaaag atgttgcaag attggcagga
    12901 gtatctcaat catctgtttc aagagcattt ggtagtgcaa atggaaaggg cgtaaaacct
    12961 gaagtaagag aaagaatttt tcgtgtagca aatgaaatag gatatgttcc aaatttggtt
    13021 gcacggggaa tgattagtgg aaaaactaat gttataggat tgatagttgg tgacagtcta
    13081 ggtccatttt acaatagaat tatcaattta tttgtagaaa aaattcaaga gataggtaaa
    13141 cagtgtcttg tttttaaagt tccgagacaa caaagtatag atagtataat ttcaaaagta
    13201 attcagtttc aagtagaagc tgtgattatt acagcttcag ccatgaataa ggtgatggct
    13261 gaaacttgtg aagaaaataa tattcctgtg attcttttta atcgttttat acctggtata
    13321 aaaataagta cagtctatgt agacccaatt gaaggtgggg gaatggctgc tgaatatctg
    13381 cttaagaaag gtcacaagaa tataggatat attcaattta cgagagaaac aagtgaggaa
    13441 atggaaaaga aaattggatt ctattctaag ctaagacaaa gtgggattca taatcttaaa
    13501 gaagaatctg caaactatga ttatgattct ggatatgaag caggtaagag aatgttagca
    13561 ttatcaaatc ctccaacagc aattttttgc acaagtgact tgatagctat tggtgtaatg
    13621 gatgctgcaa ggtttgagta taagttgagg gtgccagagg atttatcggt gataggatat
    13681 gatgatattc aaatggcttc atggaagagt tataatttaa cgacgattag acaaccacta
    13741 gatttgttga ttgaaaaaac cattaacata cttaagtcct tattaaatga agaaaatcca
    13801 gagtcagttg ttgaaatgtt aaaaccggaa ttgattgaaa gaggttctac actttaagtg
    13861 atagatattt aattaagtga taaatgttta atcaagtgat agatacctaa ttaagttgta
    13921 gatgtctgat aagaaatttt taagtttcca attgttctaa attgtgagta taaaaatcta
    13981 ctaaatttag ttatgtaaaa tataaattat aggtgtagaa actaggctac tagcttttat
    14041 accacttcaa acattactgg aggtagttaa gtttacagca gtttagtttt atgtagatat
    14101 aatattatgc aaatagacaa aattaaaata tggaagtgtc tcaaaatgaa ttttttagtt
    14161 tatgaggcac tttcttgtat gaaatcaaat atatttttat aactttaaca ataaaaagag
    14221 gcttatctct attttgagac agcctcaaat ttttaaaact atactagttt acagcttctt
    14281 ttcctatcaa atcttttaac tcttcatgag tcatagtttc tttttcaaat aagaatttag
    14341 aaacagaatg taaatcttca agattatcct ttaacatctc taaagtctct ttataacaaa
    14401 cttcaactat acgatttgct tccttttgaa ccttatcaca taagaaagtc ttatcatttc
    14461 catcaatagt cataaatcct aaattactca taccatattc acaaaccatt tggttagcta
    14521 tttcagtgac tttctttaag tcgtcctttg caccagtaga tttatgatta aatattaact
    14581 cttctgaagc ctttccagca agtaatattt ttatttttcc aatcaactcg tccttagtat
    14641 aaatatatct atcttcatca ggtaattgaa gaacataacc taatgcttgt cctctaggaa
    14701 ctatagatat tttttgtata ggacaaattc ctactatatc acttacaaga gcatgtccag
    14761 cttcatgata tgctactatt tttttctctt tttcaaccaa tgcagagttt ttagattcta
    14821 gccctgcaat aactctttca agtgccttgt caaaatgttc tgatgtaata cactcactgt
    14881 catctctaac tgcaaatata gctgcttcat ttgctatatt tgaaagatga gccccattga
    14941 aaccatgagt ttttttagct aaaagttcta gatttacaga tttgtctata ggtttgtcat
    15001 ctgtatgtac ctttagaatt tcaaatctag tgtgatagtt tggtgcacct atatgaatgt
    15061 gtctgtcaaa tcttcctggt ctaagtaaag cttcatctaa taagtcaagt ctattggtag
    15121 cacctattat taatacattg gaatctttat taaatccatc catttcaact aaaagttggt
    15181 ttagtgtttg gtctttttcg ttgttacttt caagatgtct ctttgctcct acagcatcta
    15241 tttcgtctat aaatattata cttggagctt cttttctagc tttttcaaat agagttctaa
    15301 ctctttttgc tcccacacct acatattttt caacgaactc tgaaccggta acattgaaaa
    15361 atgatgagtt tgtttcacca gctacagcag atgcaagtag ggttttacct gttcctggag
    15421 gaccataaaa taatactcct ttaggtattt ttgcacccat tttttgatat ttttgtggtg
    15481 acttcataaa atcgactatt tcaaatagtt cttcttttac ttcgtctaat ccagcaacgt
    15541 ctctaaatgt agtctttggt tttgtagaaa gtgaatcatc tgtttgatta tctctttcca
    15601 tttttgtctg tgcaaagaca ggagctggat tgttgatttt cttaaaaaag ttgtttagca
    15661 ttttcatatt cacacctcag gtaattaatt attgttaatc aattttatat tttccatatt
    15721 tactattttt tattatttat ttttcatgga aaaaatatac atgttatcta aataaaaata
    15781 aatttgtaag atgtatgaaa aagtgaattt atgtatatag agatttctat ttatgtattg
    15841 aataaagtat tacaaattat aaattatgta ttgattatat attgagtaag gtattataaa
    15901 gcatgtattg attaattatt gattatgcat tgaataaaat attacaaatt tagctggaat
    15961 aaaaatgata aaaaatgttg tactatattt tacaaatcat ataaatagag aaattgttgt
    16021 agcgcaaaat agtgggtagt atattagaat ataatgtagg atttgttgtg tagtatagta
    16081 gcatattaga atataatata gggtttgttg tgtagtatag tagaatatta gaatataata
    16141 taggatttgt tgagtagaat attagaatat aatataggat ttgttgagta gcatattaga
    16201 atataatata gaatttgttt ggtagtatag tagcatatta gaatataata taagatttgt
    16261 tgagtagtat agtattatat tagaatacaa tataggattt gttatgtaga atatataaag
    16321 tcatgtagaa tatacctata taaaaggatt ttgggtataa atttaaaaat taatagcaaa
    16381 atgagtatta tagacatata attagtagta ggaagtaatt aaatttttcc taaacaaaaa
    16441 aaatagttgc aaataatttg attaagtgat atagtaaata aggtaagaat aaaacaaaga
    16501 agaagtccgc ttctcacctt acgccagagg ttagtaaggt aaatataagt taaagcttaa
    16561 ttttaattta agtttgttta aatttggtaa cttatattgc ggatgattac gtcatccgct
    16621 ttttatttta taaaaatttt ggaggtgtcc tacaattagc aaagaattag caatcaatga
    16681 acaaataaaa gataaggaaa taagagtcct ttcgccaaca ggtgaacaac ttggagtaat
    16741 gcctactaaa gaagctcaag caatggctaa tagcaaaaac ttagatttgg ttcaaatctc
    16801 gccaaatgca aatccaccag tatgtaagat aatggattat ggaaagttta gatatgagca
    16861 agcgagaaaa gagaaagaag ctaagaaaaa acaaaaaact atagttgtaa aggaagtaag
    16921 acttagacca ggtattgaac aaaatgattt aaatacgaaa gctaataatg ctataaagtt
    16981 ccttaaaaaa ggggataaag tcaaagttga acttagattt agaggaagag aattaggaca
    17041 taaagacata ggtaaagaag ttatgcttaa gtttttagat ataataaaag agtttggaga
    17101 accaacaaaa gcccctgcat ttgagggtaa taatatggtt gtaattatag atccaaaaaa
    17161 atagatgatt aactgagagg aggaatttgt catgcctaaa atgaaaactc atagaggtgc
    17221 agcaaagaga ttaaagaaaa caggaactgg aaagttaaag agagctaaag cttttaaaaa
    17281 acatatatta actaaaaaat ctgcaaaaac taaaatgaac ttaagaaaat caactttagt
    17341 aagtgatgga gacgctaaga gaatagcaca attattacca tactaagata cagattataa
    17401 taataactat agaagaggga tataaaagga ggtcataacg atggcaagag ttaaaaaagc
    17461 tatgaacgct cgtaaaaagc ataagaaaat attaaaactt gcaaaaggat tcagaggatc
    17521 tagaagcaaa ttatatagac cagcaaacac attcgtaatg aaagcattaa aaaatgctta
    17581 tataggaaga aagttaaaga agagagactt cagaaaactt tggatacaaa gaataaatgc
    17641 agctgctaga atgaatggaa tatcttattc aagattaatg aatggattaa aattatctgg
    17701 tgttgaagtt aacagaaaaa tgttatctga aatggctata caagacccag aaggatttgc
    17761 aaaattagca gaagttgcta aagcaaaatt agcttagtat tgaatcatga gatgtaaagt
    17821 ctttgagtta tataaataat tcaaggactt ttttattttt tgaaaaatac ttattagtgt
    17881 catgtacaag caattttata atgataaata aaataagcaa tacaatagaa ttttctgata
    17941 tttattataa aaattgttac aattatttat gatagctata aaaagtcata gtaaaaatga
    18001 tattgatata tgttgatata tgtataaagt ttccaatcaa tcttgaatta atattatcaa
    18061 catgataata ttatcttatt aataatataa ataaggggga gataatatga atgagaaact
    18121 aatggattgc tttgtaaacc caattaaatg caaattatta cttgaaattt tttccaataa
    18181 tcgaactaca gctaaacaat taggagaaaa atttcaagat atatcaaaag caacacttta
    18241 tcgttacctt aataaaatgc ttaaagatga tattataaga gtggttgaag aaaataaaat
    18301 aaggggaact gttgaaaaag tatatgctat gaagttagat atagctgatg atgtgaacaa
    18361 aattatagat gagaactctg gagaagaata tgctcaactg tttactcaat atatgatggg
    18421 atttttaaga gaattttatg aatatgctga aagaaaggaa aaggatatct taaaagatgg
    18481 ttcaggattt tctgtctgtc cagtatatgc tagtattgat gaaatagttg aagtgagtaa
    18541 aaaaattgct agtatactag aacctttaag aaataattca cacgaagaag gtagaagaat
    18601 gcattctata ggaatgataa ttactccacc taaagaggat gaaggtaaat aaatctgatt
    18661 tacatgaggt agagaccatt tatacaggga atcgttttat gattcacagt atgtatccag
    18721 agcaaaatat ctctgcttag attatacttc attataccat ttcatctaat gacttggagt
    18781 ggtacaatga agttttttaa tttttcatat caataattaa agaaataaac tttagaccac
    18841 atatacaatt aggttacatt atacaattag accagatata caaaataaaa attagtattt
    18901 ttatagcaga caaattgact gttattcttc ttaaaaatca tctattagtt attgttagaa
    18961 tattgaaaga aattatagag acctattgga taaatgaata ttgcccatag tcatataaac
    19021 tgctatagtt taaaataaag caggacgtta aattttatta taatctaaaa atttaagagg
    19081 gggattttat gaaaataaaa tttgttatta tgccattagt gatattttta atggtattat
    19141 ttgtagggtg tcaaagtaca aaagatacag aagttaaagc aaaagataca gaaattaaaa
    19201 tattaggtac aggtgatttg cattcaattt tgacagactc tatagtttca tatgtaaatg
    19261 aggaaaggga aaagaataaa aatctgctta tggtagatgc gggagatttc tatggtacac
    19321 aaagtagaga aatgtgggaa tggtcttctg gaaaaaagtt aattaatatt agaagaaatg
    19381 gtagagcaga gtatgatgac ataattaaga atagtaaaga tgaagtacct atagttaaag
    19441 atatggcaaa gttgaaatat gatgctgtag ttttgggaga taatgaattt gtttcaaatg
    19501 ataaacaaag tcttgataaa ctagtttctg attttaaaaa taataatatg ccattggtat
    19561 cagcaaatat atatgaacaa tctggagaaa attatgtaca accatatgta atgaaaaata
    19621 ttaagacaga tgggggcaat gtaaaagttg gaatactagg tcttactata aaagaagtag
    19681 gggaaaccct tggtcttgaa gattttgaaa aggataaaaa agcaagagag cttggagagc
    19741 aagctgatta caaaggaaaa ttatatgcaa atgatttagt agaagatgca aagaaatgga
    19801 taaaagttat ggaaaaagaa agtccagaca taatagttgc agttgtacac tcaggggaag
    19861 aacctaaaac agatagaaat ccaggaaata gaataaaaga gttagcaact acagtggatg
    19921 gaatagatgc aattgtagct ggtcatactc atgaaaaaat agatgaacat aaatataaaa
    19981 ataaatctgg agatgaagta attgtcacac aaccaggttg gcatggagat agagtgtcag
    20041 aggttagttt caaattaaat aaagagaatg gaaagtggga cataaaggaa aagtctagta
    20101 aattaaaaat aattgataaa gaagtaagta tagttgctac tgcagattta cattcacatt
    20161 tctctgataa attaacagtt gaccttgtta atgaacgaag taacccaatt gaacctgttg
    20221 tagtagatgc aggcgacttc ttagaccctc aaacagatga aatgactcaa tggtataaag
    20281 agtggaaaaa tataagagat aataatagtg gcaaaacgat tagtagatgc ccaatggtca
    20341 tggatatgaa tcagggaatg tatgatgcag tagtacttgg gaatcatgaa tttgtatctg
    20401 aaaaagatga attttggtca gatgaacgcc ttttagatgc tgttatagag gattttgaac
    20461 aggtggcaat ttctgtgtta tcggcaaata tatataggca atctggtaaa aattatgtta
    20521 aaccatatat tataaaagat gtagagacga gtgaaggtaa tgtaaaagta ggaatattgg
    20581 gacttactat aaaagaagta ggtaaaggtc ttgaaaatgt aaatttacaa gagcaatttc
    20641 aatataaggg taaattgtat gcaaatgatt tagtagaaga tgcaaagaaa tgggtaaaag
    20701 ttatgaaaaa gaaaaaacca gatataatag ttgcagttgt acattcagga gaggagccaa
    20761 aaaatccaaa acatccagga aatagaataa aagagctagc aactacagta gagggtattg
    20821 atgcaattgt tgctagtcat actcatgaaa aaatagatga gcatgaatat aaaaataagt
    20881 ctggagagaa agttatagtg acacaaccag gtgagcatgg taaatattat tctaaaatta
    20941 cttttaaagt gaataaagaa aaggattcat ggattattaa tgataaattc agtaagacag
    21001 ttgaggttga ataactttat aaaatttata gataaaataa attatatgat attagtatga
    21061 tatacaagat aaagttttta atattagtat aatgtgaaat attctagcaa taagagaata
    21121 aacaagataa ataaaaatat ttcaacatgt aagatatagt atgaacatat tatactaatg
    21181 cttactttat aatttgagca tgttagaata agtcttattt gacaatttta gtgcactaga
    21241 gtaagtatta ttttaccagt aaaaaatatt gagaactata tgtagatttg cttaaaaagg
    21301 aatttacata tagttctttt cgtataataa atagtacaat cttatatggt aaagataaag
    21361 tgtgatatta aattttatta tcatccaaaa tctaaggagg agtctcttat gaaaataaaa
    21421 tttattatag tagcatcaat aatattttta atagtattac ttgtggggtg tcaaagtact
    21481 aaagatacag aaatcaatgt actcgccaca agtgatttac attcaattat tccagataat
    21541 ttaatttcct atgtagaaga ggagagaaaa tatgatgaga atctacttat ggtagatgca
    21601 ggagattttt ttgacatgca aagtggtgag atgaataagt ggtttactgg tcaaaaacta
    21661 gtaagattta aagatgggat accagagtac aaaaaaatag ctgaaccacg tgaaggagaa
    21721 gtaccattgg ctaaaaaaat ggcaaagctg aaatatgatg cagttacatt tgggaatcat
    21781 gaatttgtat ctaatgacaa gcagagcctt gataaattgg tttctgattt taaaaataac
    21841 aatataccat tagtatcagc aaacgtatat gaacaatctg gagaaaacta cgtaaagcca
    21901 tatgtaatga aaaatataaa tactgacaag ggatctgtaa aggttggtat acttggtctg
    21961 actataaaag aagtagggga acgttcaagg tttaaaagtt ttgaacaaga taaaaaggct
    22021 agagaacttg gggagcaacc tcaatacaaa gggaaattat atgcaaatga tttagtagaa
    22081 gatgcaaata agtgggtaaa ggtcatggaa aaaaaagaaa agccagatat aatagttgcc
    22141 attgtacact caggtgaaga accaaaaaat cctaaaaatc caggaaatag aataaaagaa
    22201 cttgcaacaa ctgtagaggg aatagatgct attgtagcag gtcatacaca tgaaaaaata
    22261 gaacagcata catataaaaa taattctggt gaagaagtaa tagtaacaca gccgggagct
    22321 catggtaata gtatatcaaa gattaatttt aagttggata agaagaatga taagtggttt
    22381 ataaaagaca aatatagtga attaaaggtt tttggaaagg aagtaaatat aattacaact
    22441 tctggtctga atccaaaatt ttcagatgaa atggcagtta atttgtttaa tgaaagggat
    22501 agagatgttc aacctatctt tgtagatact ggagattttt taaatactaa tacaaaagaa
    22561 atgactaagt gggctaaaga atggcaatac aatgaaaata aaggtatcta taaaaaaata
    22621 aatgaatggc caattgtata tcttggttat gatgctgtag tacttggtag ccatgaattt
    22681 acagaggata aagagacttt tgaagaaaaa aaatctacat tggattatat tatagaaaac
    22741 tttgaagaaa tgaagatacc agtattatca gctaatatat atgatgaatc tggagaaaat
    22801 tatgtaaaac cttatataat tcataatgta gaaactaggg aaggaaatat aaaggttgga
    22861 gtattaggtc ttactactac tgataaggat gaagaagact taagtagctc aagcttaagc
    22921 aaagattcaa agacttcgaa aattcctgaa gaaacaggaa agttatacac taacaatcta
    22981 gtaaaagatg caagcgaatg ggtaaaagtt atgcaaaagg aaagtcctga tgtgattgtt
    23041 gcagttgtac attcagataa taatagtact ctaaaaaatc caagtagtga agtaaaaaaa
    23101 cttgcaatgt cagtagatgg aatagatgct attgttgtag gtcatacgaa aaataaggta
    23161 gaagaacata tttacaagaa taagtctggg gatgaggtca tagtaacaca atctggagaa
    23221 gactgttatt caaaaataag tcttgaacta agaaaagaaa atggcaagtg gaatatcata
    23281 aacaaatata gtaaatcaat aaaaatgaag tgaatttatt gatatataaa ccattattaa
//
LOCUS       DAEPXF010000050        23259 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain HB27
            SAMN13376389-rid12479423.denovo.053, whole genome shotgun sequence.
ACCESSION   DAEPXF010000050 DAEPXF010000000
VERSION     DAEPXF010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13376389
            Sequence Read Archive: SRR10518432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 23259)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23259)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001069624.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001069624.1
            Genome Coverage       :: 315x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/15/2021 01:54:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,165
            CDSs (total)                      :: 4,076
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 89
            rRNAs                             :: 10, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 10 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 19 of 69
            Pseudo Genes (incomplete)         :: 44 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 10 of 69
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23259
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13376389-rid12479423.denovo.053"
                     /strain="HB27"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="China: Hebei"
                     /collection_date="2017"
     gene            complement(132..1088)
                     /locus_tag="KRL67_001676"
     CDS             complement(132..1088)
                     /locus_tag="KRL67_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HBH1522559.1"
                     /translation="MENNTIQKQLRDLSKKIWHIYILNPEEENLKFIISLLDDNLALI
                     GTGKHEFYETLEEFIIGLEKDQTESEQIDFEIIDEWYSCTKVTDEAFIVYGTLWVIEK
                     DYLEKDIYVEMDTRFTMVYRITDDVIKLIHVHHSIPNFDQMKDEYYPKTITEKAQEAL
                     AMANEFKLKSEKDLMTGLYNRHFFEVHIKEMLKTVSHGYFYIFDLDNFKQVNDIFGHV
                     RGDEILIHFSKILTKVFVNDAIIGRLGGDEFAVFEYSNQSKENVENKAKKVLELCLES
                     NSCSIGISKLENNSDTFQSLYKQSDYALYYSKRNNKGNFSWF"
     gene            1791..2786
                     /locus_tag="KRL67_001677"
     CDS             1791..2786
                     /locus_tag="KRL67_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBH1522560.1"
                     /translation="MSIIELNELTKSYRRFEKKAGFMGSVKSLFNRKYTEKTAISSFD
                     LKIEEGEFVGLIGQNGAGKTTLIKMLTGIIAPSSGEISVMGYYPNKLENNFKKQYAVV
                     MGQKSQLFFELTPADTFLLFKELYDISNEEYKKNLDYFIELFEVSDYLNVQVRTLSLG
                     ERMKMELIVALLHNPKILFLDEPTIGLDAIAQKQIRYFLKEVNETKGTTIILTSHYME
                     DIKSLCSRSIVVENSHKIYDGSTERLFNHFQKYKKITVSFSNETNIIFPKDCKVVEQT
                     NHKTSVIVPKERGNETIKWIFDKYDVKDAMIEEEDIGSIVERIYMKGSDEYYEQL"
     gene            2764..3627
                     /locus_tag="KRL67_001678"
     CDS             2764..3627
                     /locus_tag="KRL67_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 family transporter protein"
                     /protein_id="HBH1522561.1"
                     /translation="MSIMSNYNEASSNSSMFYKFKQSIRRYYEVAKINFKVQLIWRFD
                     VVVNMVFTIVKILFAYILWKAIFGENKIVSGFTFNSMLSYYIISSFLAGIDMSKSTSE
                     EICNRVKLGTFSKYMIIPINVQNYFIAKNFGMTCFYFVFNMISAFVWIFIFKIDFAFT
                     SNPVQILISILMIFLGFIFMMKLHFFIGILSFKFLEISFFRMIIENIVEFITGTMIPL
                     VLLPETIVTVMKIFPFYYTTYLPSMLLIGRNSNEAIQGLIILALWVLFFSVVNKVMYK
                     RMRTLFDGVGI"
     gene            3624..4418
                     /locus_tag="KRL67_001679"
     CDS             3624..4418
                     /locus_tag="KRL67_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 family transporter protein"
                     /protein_id="HBH1522562.1"
                     /translation="MIKKNLKIIFMLLKMKLSKMMVFRFDFFGAFFVDSSLFILQLLM
                     FNSIYSHVDSIGGWQQGEMLIFIGTFSLINAINMTIFFFGIYDIPRKIQEGELDYYIT
                     KPLDPLFRLTFENINIGSSPLILASILIVLYGISELNSEITLVMIIIYIFMVLLMTLL
                     FYDVCIILRTVSFFVISSSAIMRLEDNLLPMNMKIPGVIYEGVFKVLFYLILPYGIMS
                     TIPTQILAGTITINGLIYSILIVFLFTLFMWRFWKLGMRHYKSASS"
     gene            4703..6082
                     /locus_tag="KRL67_001680"
     CDS             4703..6082
                     /locus_tag="KRL67_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HBH1522563.1"
                     /translation="MSDVYYSENHEQDNLYEQDNLLAQRFNLFTLLKFAFPTIFMMIF
                     MGLYTIVDTIFVSRFINTNALSAINIVCPIINLIVGIGTMLATGGSAIVARKMGNGDI
                     EGANKAFSLIVLSGVVIGVIITGIGIVFMDYIIIWLGASEVLIKYCRSYLFIILIFAS
                     ANILQVLFQCLFVTAGKPQFGLWLVLGAGCANIILDYVFIVLLQMGISGAALATGVGY
                     LIPTISGMVFFSKNKGLLKFCIPRLDKRILFEACFNGSSEMVGQLSTAVTTFLFNAMM
                     MKLIGEDGVAAITIIIYSQFLLTSLYIGFSMGVAPIISYNYGSENNTQLNHVFKICIL
                     FISVASLLVFIFSISCSPIIVEIFSQRGSRVYEIARSGFYIFSFSFIFCGYNIFTSSM
                     FTALSNGKVSAILSFLRTFGFITIGLLILPNILNINGIWLAVPSAEILTLFLTVFCIC
                     KYKNRYKYM"
     gene            6532..7245
                     /locus_tag="KRL67_001681"
     CDS             6532..7245
                     /locus_tag="KRL67_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBH1522564.1"
                     /translation="MDTYFNKKILLVDDEKDIVDLIEEVLINDGFKNIIKAYNGLDAI
                     SLCKIACPDVVILDIMLPDIDGIEVCKKIREFSYCSILFLSSKNDDIDKILGLSSGGD
                     DYITKPFSPREIVFRVKAQLRRQQYQSIVPSDSEVIKVGDITIDIEGNRVYKDKNEIE
                     LTGREYHLLSYMAQNVNKIIGKERLYEQVWGVYSSICDNTIMVHIRHIREKIEDNPSN
                     PKILITVKGLGYKLVNRID"
     gene            complement(7523..9061)
                     /locus_tag="KRL67_001682"
     CDS             complement(7523..9061)
                     /locus_tag="KRL67_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HBH1522565.1"
                     /translation="MSLLKVTNLSQCFMDKSLYEKANFDLFKGEHIGVVGQNGAGKST
                     LIKILLGEVVPDSGEIKWQPNINIGHLDQYAEINKDTTISLYLHTAFEKLYKIEKEMN
                     LLYQKSAISGNEQHLIKASDYQEQLIANNFYYIDNEINKIANGLGLDSIGMNRVVGEL
                     SGGQRAKVILAKLLLSNHDVLLLDEPTNFLDKEHVEWLSDYLNTFNGAFIVVSHDFDF
                     LEKISTGILDIEFGMIKKYHGKYSEFLKQKSHLREDYIRRYQAQQKKIEKEETFIRKN
                     KAGVNSKIARGRQKQLDKIERIAPPSFTGKPNIQFSEIEISAQNALTITNLEVGYYYS
                     LLPKLNFSVDGGQKIVITGFNGIGKSTLLKTLVKDIPRISGDFQFSEQVKIGYYEQDL
                     KWENPDKTPLQIVADKYPKLNTKEIRRHLARCGVKEEHVSRSVSTLSGGEQSKVKLCC
                     MMLSPCNFLILDEPTNHFDAETKDALQNALKQFRGSIILVSHEEKFYKGWIDKVFNIE
                     KQLV"
     gene            9943..10398
                     /locus_tag="KRL67_001683"
     CDS             9943..10398
                     /locus_tag="KRL67_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HBH1522566.1"
                     /translation="MDEIDLKIINSLKKNSRASTSEISRQINLSVPAVSERIRKIEQS
                     NLIQKYTIQINREKTKYKLLAFIFINTDLSKSMKEFKKTILEYDTVLECHHISGEYDY
                     LIKVLAEDTSNLEEFISVTLKEIGGIQKANTTIVLSTIKEEFNTIKDIF"
     gene            10447..11031
                     /locus_tag="KRL67_001684"
     CDS             10447..11031
                     /locus_tag="KRL67_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DJ-1/PfpI family protein"
                     /protein_id="HBH1522567.1"
                     /translation="MKILVFLAKGFETMEFSVFVDVVGWARNDYGHDIDVVTCGFKKQ
                     VMSTFNIQVLVDKTIEEVCVDDYDALAIPGGFEEFGFYDEAYDSSFLNLIREFNSKEK
                     IIASICVAALPVGKSGVLKNRKATTYHLKNGKRQRQLSEFDVNVVNEPIVVDKNIITS
                     YCPETAPHVAFKLLEMLTSKEQMDEVKLAMGFKL"
     gene            complement(11036..11359)
                     /locus_tag="KRL67_001685"
     CDS             complement(11036..11359)
                     /locus_tag="KRL67_001685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBH1522568.1"
                     /translation="MKKCLDSYSCPIEATLALIGGKYKTLILWHLKDTILRFNELKKL
                     IPKATPKMLTQQLRELESDGLIIRVVYPVVPPKVEYSLSDFGKSIIPILDSMCDWGSD
                     YLEKL"
     gene            11511..12029
                     /locus_tag="KRL67_001686"
     CDS             11511..12029
                     /locus_tag="KRL67_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HBH1522569.1"
                     /translation="MNFVELAKKRYSCRNYQDRKVEKEKLEKVLDVARIAPTGGNRQP
                     QRLIVIQEKEGINKLSKAANIYDAPLAILVCGDKDKVWTRPFDGKQLTDIDTSIVTDH
                     MMLQATELGLASVWVCYFNPDIIREEFSLPDNLEPINILLMGYESKIPESPERHEKTR
                     VPLSEIVSYETL"
     gene            complement(12205..12873)
                     /locus_tag="KRL67_001687"
     CDS             complement(12205..12873)
                     /locus_tag="KRL67_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH1522570.1"
                     /translation="MLEISHKVKEIYPNIKFGVMVINITHSKPNKENFLILKDSTIKN
                     IIEQHPEYNRKEKIKTEPASSYIKYYKKFKKTYPVLLQLESILLKSKGIPDVDVTIES
                     MFLAELKNLLLTAGHDLDKIELPLKIELANGSEHFYGIGGKEQILTKDDLFLSDNIGI
                     LSSILNGPDNRTAITKDTKNIMYFVYGPDKISEKQIQDHLNDIKHYISSAFPDLKVNS
                     IDIF"
     gene            12988..14403
                     /locus_tag="KRL67_001688"
     CDS             12988..14403
                     /locus_tag="KRL67_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HBH1522571.1"
                     /translation="MWITINRNSNISLSRQIYCEIKDMILDGSLKFEQKLPSSRTLSK
                     ELSVSRNTVLDAYNQLIAEGYLETRNGYGTIVAKGISGHQVDFKLNYTFRNEKDLHTN
                     KEKNYIDFRSGVPELKLFPRKELSKLYKQILYDLSDSDFRYGSTAGVWVLRVEISKYL
                     FRTRGIRCNPKNIMIVSGSTQGLSLISQLLYKSNKKVAVEDPIHKGLLKVISSIGYSV
                     VGIKVDDKGMNTNLIKSIKGISFVYTTPSHQYPLGGILPIQRRLELIKCATENDYYII
                     EDDYDSEFRYEGQPISSLYELNPNKVIYIGSFSKILSPAIRVGFMLLPDNILESYKTL
                     KMYSDVHTETILQHVLAEFIGNGGLEKYIFRMKKLYNRKRKYLIKVLNTEFKNEFKIK
                     GEAAGLHIVAHFYNVKFTESLIHKIFEAGIRVYPIQQCAIENILEYGHEIILGYAHLS
                     FDEIEKGVRILSKIIHMNQGT"
     gene            14626..15084
                     /locus_tag="KRL67_001689"
     CDS             14626..15084
                     /locus_tag="KRL67_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBH1522572.1"
                     /translation="MEIIDYIEENLTENLNLDKIADIANYSKYHLHRMFTNVVGCTIH
                     QYIQRRRLTEAARQLIFTDKYIIDIAITVGYSTHQSFTLAFRNLYKESPQVYRNKDEF
                     YPIQQRFVLNLNHQIQSKKCISYANMNYKQRRDEVNSYLNFNINCEVEVA"
     gene            15081..15665
                     /gene="clpP"
                     /locus_tag="KRL67_001690"
     CDS             15081..15665
                     /gene="clpP"
                     /locus_tag="KRL67_001690"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HBH1522573.1"
                     /translation="MSLVPHVIEQTGQGERSYDIYSKLLKDRIIFIGEEINDAIASLV
                     VAQLLFLESEDPDSDIIIYINSPGGSTTAGFAIYDTMNYVRCDVSTICIGMAASMSAF
                     LLAGGTRGKRCALPNSEIMIHQPMGGAKGQATDVKIAVDNILKIKEQLDRILSENTGK
                     SIEDIRRDTDRDNFMTAIEAKEYGLIDYIMNRNE"
     gene            15977..17053
                     /locus_tag="KRL67_001691"
     CDS             15977..17053
                     /locus_tag="KRL67_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HBH1522574.1"
                     /translation="MITISLCMIVKNEEDVIGRCLECVKDIVDEIIVVDTGSTDSTKE
                     IVSKYTDMVYDFEWIDDFSAARNFSFSKASKDYIMWLDADDIILEDDREKLLKVKETL
                     DTSIDIVMAKYNVSFDDNGKPTLSYYRERLFKRSSNHKWVDPIHEVIPLYGEIFYSEI
                     AISHKKLHRQDPLRNLRIFEKMISEGKVLGPRHQFYYSRELYYNARYEEAIEGFTKFL
                     DSSRGWLEDCISACRDLATCYYLINDEKSALYSLFRSFEFDEPRAEVCCDIGKHMFDR
                     QKYKEAIFWYKVALTRDKNDTNGGFKSNDCYGYIPSIQLSVCYDRLGESDKAIYYHEK
                     TKEIKPNDSAVLHNENYFSKFKNS"
     gene            17120..19258
                     /locus_tag="KRL67_001692"
     CDS             17120..19258
                     /locus_tag="KRL67_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore surface glycoprotein BclB"
                     /protein_id="HBH1522575.1"
                     /translation="MLLIMSRNKYFGPFDDNDYNNGYDKYDDCNSGRDDYNSCDCHHC
                     CPPSCVGPTGPMGPRGRTGPTGPTGPTGPGVGATGPTGPTGPTGPTGPTGNTGGTGTG
                     GPTGPTGPTGPTGPTGNTGGTGTGGPTGPTGPTGPTGNTGATGFGVTGPTGVTGATGA
                     TGADGVTGATGPTGATGADGATGPTGATGATGFGVTGPTGPTGPTGVGVTGATGLIGP
                     TGATGTPGATGPTGAVGATGIGITGPTGPTGATGADGATGVTGPTGATGATGADGVTG
                     PTGATGATGFGVTGPTGPTGATGIGITGATGLIGPTGATGATGATGPTGVTGATGAAG
                     LIGPTGATGATGADGATGATGATGATGPTGADGLVGPTGATGATGADGLVGPTGPTGA
                     TGVGITGATGATGATGPTGADGLVGPTGATGATGADGVAGPTGATGATGNTGADGATG
                     PTGATGPTGADGLVGPTGATGATGLAGATGATGPIGATGPTGADGATGATGATGPTGA
                     DGLVGPTGATGATGATGATGATGPTGATGPTGASAIIPFASGIPLSLTTIAGGLVGTP
                     GFVGFGSSAPGLSIVGGVIDLTNAAGTLTNFAFSMPRAGTITSISAYFSTTAALSLVG
                     STVTITATLYQSTAPNNSFTAVPGATVTLAPPLTGILSVGSISSGIVTGLNIAATAET
                     RFLLVFTATASGLSLVNTVAGYASAGIAIN"
     gene            19430..20191
                     /locus_tag="KRL67_001693"
     CDS             19430..20191
                     /locus_tag="KRL67_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase"
                     /protein_id="HBH1522576.1"
                     /translation="MKNKIETLMNNFVKNYHKSNNITTKWQELLFAYASAEDDMFQKL
                     KSVVGTSHVMPKDFLKNGKTIITYFIPFDESVNNSNIGGFECSEIWARAYIETNMLIS
                     NLNDFLCEELEKIGANSYAIPGSYNFDSEKLVSDWSQRHIAFIAGLGTFGLNNMLITE
                     KGCCGRIGSIITSLELEPTKRPENEYCLYKHNNSCKKCVNKCVKEALKEDGFNRNKCY
                     EMCMINEKKYTYIGGCDICGKCIVGVPCSVINPSR"
     gene            20500..20778
                     /locus_tag="KRL67_001694"
     CDS             20500..20778
                     /locus_tag="KRL67_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH1522577.1"
                     /translation="MSYKKAIHVLPEELLELIQEYVDGECIYIPRKSNNKKEWGSNTP
                     TREELAIRNMQIYEDYQVGYDLQYLSEKYYLSLKSIQRIVLQEKRKDI"
     gene            21071..21646
                     /locus_tag="KRL67_001695"
     CDS             21071..21646
                     /locus_tag="KRL67_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1062 domain-containing protein"
                     /protein_id="HBH1522578.1"
                     /translation="MQKITWEVEYISQLPVIRYCKKCGKKTEYICSGLFRINAQHKYL
                     DIWLIYKCSNCDSTWNSTIYSRVNPKSLNPETLEQFHTNDYNLAKQYAMNMELLHRNG
                     AEVGIPEYKIVGQEININDLTQVHIISKYPSHLKVSTLLREKLGLSRKVFDEMLDCGI
                     IRSESGVDLKKGKLNYEIILDIGKEKQKSSN"
     gene            complement(21753..22586)
                     /locus_tag="KRL67_001696"
     CDS             complement(21753..22586)
                     /locus_tag="KRL67_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prenyltransferase"
                     /protein_id="HBH1522579.1"
                     /translation="MNVENQSLSDIEIILSHRYDNGADYWTTPDKRLIKGSPFSTLDS
                     VLYLLELGVNPDDTLLKECADLIFSTWREDGRFKLYPNGGIYPCHTANAANVLCHMGY
                     ASDIRMQKTFQHFLDTQYTDGGWRCNKFSFGRGPETEYSNPLPTLNILNAFRFSNYLN
                     KESKLDKAVDFLLDHWTIKKPIGPCHYGIGTLFMQVEYPFRNYNLFLYVYVLSFYNRA
                     KKDKRFLEALKILESKMIGNKIVVERVVPKLSKLSFCKKGESSEIATTHYYEILKNLE
                     K"
     gene            complement(22660..>23259)
                     /locus_tag="KRL67_001697"
     CDS             complement(22660..>23259)
                     /locus_tag="KRL67_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3786 domain-containing protein"
                     /protein_id="HBH1522580.1"
                     /translation="NYNVPFDYVRKKLKELDPHTISNLSNIPFNNDLNRFTLNFFDRQ
                     YIIDYPSGEVWNSNGSLCNNFELKILIVRYLINAKGIPRTNKYVTFKEIPGGNVYYPN
                     FLNRTLYRLSEVFTSQIDKYKLVMEKIGAEKVNMGDLAYRFTYMGNTSMVFILWFGDD
                     EFPPNSNILFDSNIVYYFNAEDLAVVPDTAIDAILNKIN"
BASE COUNT         8521 a         2908 c         3874 g         7956 t
ORIGIN      
        1 tgactattgt agttttatta agaatagttg gtttatataa atatatgcaa agataacttg
       61 ttgtttactt tatttattag tatatagtaa aagatactct aataaatttg cttattttaa
      121 tatcttttgc actaaaacca actaaagttt cctttattat ttcgttttga ataatacaaa
      181 gcataatccg attgtttgta taaagactgg aaagtatcag agttattttc taatttagat
      241 atacctatag aacaagaatt actctctagg cataattcta aaactttctt tgctttattt
      301 tcaacattct cttttgattg attactatat tcaaaaacag caaattcatc tccaccaagt
      361 cttccaataa tagcatcatt tacgaagact ttagtcaaaa tctttgaaaa atgtattagt
      421 atttcatctc ctcgaacatg accaaagata tcatttacct gtttaaaatt atctaaatca
      481 aagatgtaaa agtatccatg tgaaacagtt ttaagcattt cttttatgtg tacttcaaaa
      541 aaatgtctat tgtatagtcc agtcataagg tctttttctg attttagttt aaattcattt
      601 gccattgcta gtgcttcttg tgctttttca gttattgttt tagggtaata ctcatctttc
      661 atttggtcaa agtttggaat agaatggtga acatggataa gtttaatcac atcatctgta
      721 attctgtata ccatagtaaa tcgagtatcc atttctacat agatatcttt ttctaggtaa
      781 tctttttcta ttacccaaag agtaccatat actataaaag cttcgtctgt aactttggta
      841 catgaatacc actcatctat aatctcaaag tctatttgct ctgattctgt ttggtctttt
      901 tcaagaccta taataaattc ttctaatgtt tcgtaaaatt catgttttcc tgttccaatt
      961 aatgcaaggt tgtcatctaa taaggaaata ataaatttaa ggttttcttc ttctggattt
     1021 aaaatataaa tatgccaaat tttttttgat aaatccctta attgtttttg tattgtatta
     1081 ttttccatag attaccccct taagatttac agtttatatt ttaatgtatt ataagttgaa
     1141 ctcttattac aatattatta taatatagaa ctaaagaaat tacatcctgt ataggtaaaa
     1201 gtatatcttt tacaataaaa atattacagt aaaataaccc gttttaatat acatattata
     1261 tttttaattt ataaatgaat attataaatg aaatttaaat tcaaccctga agaaataagc
     1321 taaatgtatt agtatataaa tccatacttg aacataaata acctttatga tatgatatca
     1381 ataataatat tatgagagga gtgccttgat gtcggagtta tataatggta accaacaatt
     1441 aaatagttgg agacacatat attctgtata atcagaaatt ttaagtgtat ttttaataca
     1501 tttaattttt gtgtcttatt ctacctttat agataactga catatgacaa caaatagtat
     1561 ttaaaatata tattactttg ttaaattatg gtgtagtagg ctttaatctg ctacactttt
     1621 attaattttg tatagtatta ttttgataac tattttgaaa tagagagcat taatttttct
     1681 agtccacagg agttttctgt ttttgtgctg ttaaatataa gcacagaaaa aggcagatat
     1741 atacctgtgg acttttttta tataaattat gaaatatgga ggtagttaaa atgagcataa
     1801 ttgaacttaa tgaactgact aaatcttata gaagatttga gaaaaaggca ggatttatgg
     1861 ggagtgtaaa gtctttattt aataggaaat atacagaaaa aacagctata tcgtcatttg
     1921 atttaaagat agaagaagga gaatttgttg gattaatagg tcaaaatgga gctggcaaaa
     1981 ctactcttat taagatgtta actggtatta ttgcaccgtc ttctggagaa atatctgtta
     2041 tgggttatta ccctaacaaa ttagaaaata attttaaaaa acaatatgct gttgttatgg
     2101 gccaaaaaag tcaactattc tttgaattga caccagcaga tacgtttctt ttatttaaag
     2161 aattatatga tatatctaat gaagaatata aaaaaaactt ggattacttt atcgaattat
     2221 ttgaggtatc tgactattta aatgttcaag ttaggacgct ttctttagga gaaagaatga
     2281 aaatggaact aatagtagcc cttctacata atccaaaaat attatttttg gatgaaccaa
     2341 ctataggctt agatgccata gcccaaaaac aaattaggta tttcctaaaa gaagtaaatg
     2401 aaacaaaagg aactactatt atacttactt ctcattacat ggaagatatt aagagtctct
     2461 gtagtagaag tattgttgta gaaaatagcc ataaaatata tgatggttct acagagagac
     2521 tatttaatca ttttcaaaaa tataaaaaaa taacagttag tttttccaat gaaactaaca
     2581 ttatatttcc aaaagattgt aaagttgttg aacaaacaaa tcataagaca tcagtaattg
     2641 tgccaaaaga aagaggcaat gaaacgatta aatggatttt tgataaatat gatgttaaag
     2701 atgcaatgat tgaagaagaa gatataggta gtatagtaga gagaatatat atgaaaggaa
     2761 gtgatgagta ttatgagcaa ctataatgaa gcaagttcaa attcttcaat gttttataaa
     2821 tttaaacaga gtattagaag gtattatgaa gtagccaaaa ttaactttaa agtgcaactt
     2881 atctggagat ttgatgtggt ggttaatatg gtgtttacga ttgtaaaaat tttatttgct
     2941 tatattttat ggaaagcaat ttttggtgaa aataaaatag tctctggttt tacatttaac
     3001 tctatgttgt cttactatat aataagctca tttttagcag gtattgatat gtcaaagagt
     3061 accagtgaag agatatgtaa tcgtgtaaaa ttgggcacat tttcaaagta catgattatt
     3121 ccaattaatg ttcaaaatta ttttattgct aaaaattttg gaatgacatg tttttatttt
     3181 gtatttaata tgatttctgc atttgtatgg atttttatat ttaaaataga ttttgccttt
     3241 acatcaaatc cagttcaaat attaatttca attttgatga tttttctagg gtttattttt
     3301 atgatgaaac tccacttctt tattgggatt ttgtcattta aatttcttga gattagtttt
     3361 tttagaatga taattgaaaa tattgttgaa tttattacag gaacaatgat accacttgta
     3421 ctcttaccag aaactatagt aacagttatg aagatatttc cattttatta tacaacatat
     3481 ttgccatcta tgttactaat aggtagaaac agcaatgagg caatacaagg attaattatt
     3541 ctagcattat gggttttatt tttttctgta gtaaataaag ttatgtataa aagaatgaga
     3601 acattatttg atggggtggg tatatgatta aaaaaaattt gaaaataata tttatgttat
     3661 taaaaatgaa attatcaaag atgatggtat ttagatttga tttttttgga gcattttttg
     3721 tagatagttc attatttata ttacaacttc taatgtttaa tagtatatat tctcatgtag
     3781 actcaatagg gggatggcaa caaggagaga tgttaatatt tataggaact ttctcattaa
     3841 taaatgcgat aaatatgacg attttctttt ttggtattta tgatattcca cgaaaaatcc
     3901 aggaaggaga attagactac tatattacaa aaccattaga cccactattt aggttaacat
     3961 ttgaaaatat taatattggt tcatcaccat taattctagc aagtatactt attgttttat
     4021 atggaatatc tgagttaaac tcagagataa ctttagttat gataataata tatattttca
     4081 tggtgttatt gatgacatta cttttttatg atgtatgtat catacttaga acagtttcat
     4141 tttttgttat atcatcttct gcgataatga gattagaaga taatttattg ccaatgaaca
     4201 tgaaaattcc tggtgtaata tatgaaggtg tttttaaagt attattctat ctaatcttac
     4261 catatggaat tatgtcgaca attccgactc aaattttggc tgggacaata acaattaatg
     4321 gattaatata ctcaatttta attgtatttt tgtttacatt gtttatgtgg agattttgga
     4381 agttaggtat gagacattat aaaagtgcaa gtagttaata aattggtaaa aatagaaaat
     4441 gtataattat atgtaaatac gtttcatatg acactaaact atgtaaacaa gtatttgcac
     4501 taaaggcact gaaagctata aaatgattaa agtaaggatt taaaagttac tttagtagaa
     4561 atataattgt gtaaaaaagc aagattaatt aaaactatat ccgaataatt agatataatt
     4621 tttatgcaga aaggaggtct gtaaatgtct aattgtttgg aatatatttt aaaaataatt
     4681 aatgtacaga aaaataaaca aaatgagtga tgtatattat tcagaaaatc atgaacaaga
     4741 taatctttat gagcaagata atttactggc acaaagattt aatttattta ctctattgaa
     4801 gtttgcattt cctactattt ttatgatgat ttttatgggg ttgtatacaa tcgtcgatac
     4861 tatatttgta tcaagattta ttaatacaaa tgctctatca gcaattaata ttgtatgtcc
     4921 aattattaat cttatagttg gaattggaac tatgcttgcc actggaggaa gtgctattgt
     4981 agcacgtaag atgggaaatg gtgatattga gggagcaaat aaagctttct ctttaatcgt
     5041 attatcagga gttgtaattg gggtaataat tactggtata ggtattgtat ttatggatta
     5101 tatcattata tggttaggag caagtgaagt tttaattaaa tattgtcgtt catatctttt
     5161 tattatcttg atttttgcct ctgcgaatat actacaagta ttatttcaat gtttatttgt
     5221 aacagctgga aagcctcaat ttggattatg gctagtgtta ggtgctgggt gtgctaatat
     5281 aattcttgac tatgtgttca ttgttctgtt acaaatgggt attagtggag cagcacttgc
     5341 aactggagta ggatacctaa ttcctaccat ttcaggtatg gtcttctttt caaaaaataa
     5401 agggttactt aaattttgta tacccagact agataaaaga atattatttg aagcttgttt
     5461 taatggttca tctgaaatgg tgggtcaact atcaacagca gttactactt ttttatttaa
     5521 tgctatgatg atgaaattaa ttggtgaaga tggagttgca gctattacaa taattattta
     5581 ttctcaattt cttttaactt cgctttatat aggtttctca atgggtgttg caccaataat
     5641 aagttataac tatggaagtg aaaacaatac gcagttaaat catgttttta agatttgtat
     5701 attatttatt tcagtagcat ctttattggt atttatcttt tctatatcat gttcaccaat
     5761 cattgtagag attttttctc aaagaggaag ccgagtttat gaaattgcaa ggtctggatt
     5821 ttatatattt tcttttagtt ttattttttg tgggtataat atttttactt cgtctatgtt
     5881 tacagcatta tcaaatggaa aggtgtctgc aatattatct tttctacgta catttggatt
     5941 tataactata ggtttattga tattgccaaa tatattaaat ataaatggga tatggttagc
     6001 agtaccaagt gctgagattt taactttatt tttaaccgtt ttttgtatat gtaagtataa
     6061 gaatagatat aaatatatgt agatattaaa taaatagttt aggtagatat tttatgtttt
     6121 aataagtaat tcaattagtg gtagatagat actattgaaa attaaagtta taattgatat
     6181 aaagaattaa taaaaataac atataaaagt ttaaaagggg agttgaactt aaagtttgat
     6241 tagaaaaagt caattttaag acaactcctt ttattttact attgtattaa tctgtatctg
     6301 taagtgtatt agtctaatat tataaacttt ggatgaaatt aattttctat aggttttaat
     6361 gtatcatata ctttatttta aagaaacgca tcttaatttt cataatgatt gaatttgtat
     6421 attagagatg ttactattag taatgtaaaa gcaagagtag tgaatataaa aaaacaagat
     6481 agtgatactg caagattaga atataataaa tttttatgga gagatttcaa tatggataca
     6541 tattttaata aaaaaatatt acttgttgat gatgaaaaag atatagtaga tttaattgaa
     6601 gaagtactta taaatgatgg ttttaaaaac attattaaag cttataatgg tttagatgct
     6661 atttcgctgt gtaagatagc atgtccagat gttgtcattc ttgatataat gttaccagat
     6721 atagatggta tagaagtgtg taaaaaaatt cgagagtttt catactgttc tattcttttt
     6781 ctctcatcaa aaaatgatga tattgacaaa atactaggtc ttagtagtgg gggagatgac
     6841 tatataacaa agccattcag tccaagagaa attgtttttc gagttaaagc gcaattacgt
     6901 agacaacagt atcaaagtat tgtaccatca gatagtgaag ttattaaggt aggagatatt
     6961 actattgata tagaaggaaa ccgtgtatat aaagataaaa atgagattga gttaactgga
     7021 agggagtatc atcttttaag ctacatggct caaaatgtaa ataaaattat aggaaaagaa
     7081 agactctatg aacaagtatg gggagtatat agtagtattt gtgacaatac aattatggtg
     7141 catatacgac atataagaga aaaaattgaa gataacccct caaatcctaa aatacttata
     7201 acggttaaag gactaggata taaacttgtg aatagaatcg attagaatag attttaatat
     7261 caattaagat gtctcaaatt gaatttttta gttaatgagg cattctttta tatgtgaaat
     7321 caaatgtatt ttcgttatct caataatgaa gaagaggtct atctctattt tgagatagcc
     7381 tcattaagtt tatatcctgg caatgtactt tatgttctaa taaatattta tttttttagt
     7441 ttatttgcag tgaaacatta actagaacat taaaatttgc attgaaatac caattaaata
     7501 atattgtata ttgtgtataa acttatacta attgtttttc tatattaaat actttatcaa
     7561 tccaaccttt ataaaatttt tcttcatggg aaactaggat tatacttcct ctaaactgtt
     7621 tcaaagcatt ttgtaaagca tcctttgtct ctgcatcaaa gtgattggtt ggttcatcta
     7681 gaataagaaa attacaagga gataacatca tacaacataa tttaacctta gattgttcac
     7741 caccacttaa agtagataca cttcttgaaa catgctcttc cttcacacca catctggcta
     7801 gatgacgtct aatctctttt gtatttaatt ttggatattt atctgctact atttgaagtg
     7861 gggttttatc aggattttcc cattttaaat cttgctcata ataaccaatt ttaacttgtt
     7921 ctgaaaactg gaaatctcca gatatacgag gaatatcttt gactaatgtc tttaataagg
     7981 tagatttacc gattccatta aaaccagtta taactatttt ttgacctcca tcaacagaaa
     8041 aatttaattt tggaagtaaa gaatagtagt atccaacttc aagatttgtt atagtaagag
     8101 cattttgagc tgatatttca atttctgaaa attgaatatt tggttttcct gtaaaactag
     8161 gaggagctat tctttctatt ttatccaatt gtttttgtct tcctcttgca atttttgaat
     8221 ttactccagc tttattttta cgtataaatg tctcttcttt ttctattttc ttttgttgtg
     8281 cttgatatct tctaatataa tcttctctta aatgtgattt ttgtttaagg aactctgaat
     8341 attttccatg atatttctta atcatgccaa attctatatc aagaatacct gtagaaattt
     8401 tctctaaaaa gtcaaaatcg tgggaaacta caataaatgc gccattaaat gtatttaaat
     8461 aatctgagag ccattcaaca tgttctttat ctaaaaagtt tgttggttcg tctaataata
     8521 aaacatcatg gttagaaagt agtaattttg ctaaaattac ctttgccctt tgcccaccac
     8581 taagctctcc aacaacacga ttcattccta ttgaatctaa tccaagacca tttgctattt
     8641 tatttatttc attatctata taatagaaat tatttgctat tagttgttcc tgataatctg
     8701 atgctttgat tagatgttgc tcatttccag atatagcact tttttgatag agtaaattca
     8761 tttccttttc tattttataa agtttttcaa atgcagtgtg taaatagagt gatattgttg
     8821 tatctttatt gatttctgca tattggtcta aatgtcctat attgatatta ggttgccact
     8881 tgatttctcc tgaatctgga acaacttctc ccaataaaat tttaattaat gtacttttgc
     8941 ctgcaccatt ttgaccaact acacctatat gttcaccttt gaataaatca aagttagctt
     9001 tctcatacag tgatttatcc ataaaacatt gtgataaatt agttactttt aataaactca
     9061 taattcaaac tccttttttt tattaaaaaa tttatggaaa taaaaaaagc agaggaatgg
     9121 cattgtaaaa tgccgacctc tgcttttgga aaaacaaaag gtcatagaca agcattgttg
     9181 tctatgacca gaaaatctat attatctatc ggcaaatagg taattttaaa taagcatagc
     9241 caaaaaacta tactaaacct atatttgccg atgaagatag aaagctttaa gctttcagat
     9301 taacctctac acaacgcttc atcgtataca tttgtgtaga gttaagtttt ctaacaagtt
     9361 taactaattc tatataaatc ataattattc acatcctaat cttagtatta attctagttt
     9421 atctatattt aaattgtact tttgtacttt gtggattatc ttatttattt aaaatatgtt
     9481 ttattaatta tagcttaagt tatgttatca taatacttag ttatagtcaa taagaaagtt
     9541 atatttacta tatattatat taatttagca aatctatcat tacaaagtta tttgaagatt
     9601 cactaccaat aaagttttat agtctgtaag aaaactaaga ttatgagttt tataattttg
     9661 atatagggat actattttca taaatattat aatatgtaga agtatatgtc tagtattgtt
     9721 gaactttcaa tgatatatta aggttctaat atattataca aaataaaaac attaattcaa
     9781 cttaagaaat tttaagtatc gtatgtgttt tgattaaata cacctaaaat tttaagtgta
     9841 aataacatat ttgcattaaa ttttattgaa atgattaaaa ttatttactt aatgtaaatt
     9901 attagctaaa attgtactaa ataattaagg aggtgagtag ctttggatga gatagattta
     9961 aaaataatta attctctaaa aaagaatagt agggcttcaa cctctgaaat aagtagacaa
    10021 attaatttat cagtaccagc agtctcagag cgtataagga aaattgagca gtcaaattta
    10081 attcaaaagt acactattca aattaatcgg gaaaaaacaa aatataagct tttggctttt
    10141 atatttatca atactgattt atctaaaagt atgaaagaat ttaagaaaac tatattggaa
    10201 tatgatactg tattagaatg tcatcatatt tcaggagagt atgattactt aataaaagta
    10261 ttagcagaag atacaagtaa tcttgaagag tttatatcag tgactttgaa ggaaattggt
    10321 gggattcaga aagctaatac aaccattgtt ttatctacaa ttaaagaaga atttaataca
    10381 ataaaagata ttttttaaat aattgactag taaagtgtta attatatttt aggaggaaaa
    10441 tagaatatga aaattttagt ttttttagca aaaggttttg aaactatgga atttagtgta
    10501 tttgtagatg ttgtgggatg ggctcgtaac gattatgggc atgatattga tgtagtgact
    10561 tgtggattta aaaaacaagt tatgagcaca tttaatatac aagtattagt tgataagacg
    10621 atagaagaag tatgtgttga tgactatgat gctctagcta ttcctggagg atttgaagaa
    10681 tttggatttt atgatgaagc atatgattca tcttttttaa atctaataag ggagtttaat
    10741 tcaaaagaaa aaataatcgc ttctatttgt gtggcagcgt tgccagttgg aaaaagtggt
    10801 gttttaaaaa atcgtaaagc tactacatat catttgaaaa atggtaaaag acaaagacag
    10861 ttaagcgagt ttgatgtaaa tgtagttaat gaaccaattg ttgtagataa aaatattata
    10921 acctcatatt gtccagaaac agctccacat gtagctttta agcttttaga aatgttgaca
    10981 tctaaagaac aaatggatga agttaaatta gcaatgggat ttaaactgta atggtttaga
    11041 gcttttccaa gtaatcagaa ccccagtcac acatggaatc taaaattgga ataatactct
    11101 ttccaaaatc agatagtgag tattctactt ttggaggaac aacaggataa accactctta
    11161 taattaaacc atctgattct aattcacgta attgttgagt taacatctta ggcgttgctt
    11221 taggaattaa tttttttaat tcattgaacc tcaatatagt gtctttaaga tgccaaagta
    11281 taagtgtctt atatttacca ccaatcaatg caagtgttgc ttctattggg caactgtaac
    11341 tatctaaaca ttttttcatt tttgggctcc tttatttatt ttaaagtatc tttttaggta
    11401 ctatattaca aaaaagtgca ttattgtaat atagtaccat agtgataaaa ttatagcata
    11461 ggttaaataa atcatcaact tagcctaata aaacaatagg aggtaaataa atgaatttcg
    11521 ttgaacttgc aaaaaaaaga tattcttgcc gtaattacca agatagaaag gttgaaaaag
    11581 aaaaacttga gaaggtttta gatgttgcaa gaatagctcc aactggtggt aatcgtcaac
    11641 ctcaaagatt gattgttatt caggaaaaag aaggtataaa taaactttct aaggctgcca
    11701 atatatatga tgcgccatta gcaattcttg tatgtggaga taaagacaaa gtttggacaa
    11761 gaccttttga tgggaaacaa cttacagata ttgatacaag tatagttaca gaccatatga
    11821 tgttacaggc aactgaactt ggattggcaa gtgtttgggt gtgctatttt aatccagata
    11881 taattagaga agagtttagt ttgccagata atctagaacc aataaatata ttattgatgg
    11941 gatatgaatc taagatacca gagagcccag agagacatga aaaaactcgt gttcctttaa
    12001 gtgaaattgt ttcttatgaa actctttaat tataaaatta agttattcat aggataaatg
    12061 taataaagtg tttattataa acaaaattca aaataaataa tatctacata taattaattt
    12121 tagtgaagta tatctagttt aactagaatg tatacttcac taatttattt attcaaaata
    12181 tttatgagga ttttaatata ttaactaaaa tatatcaatt gaattaactt ttaaatctgg
    12241 aaaagcactt gaaatatagt gttttatgtc atttaaatgg tcttgaattt gcttttctga
    12301 tattttatca ggtccatata caaaatacat aatattttta gtatcttttg ttatagctgt
    12361 tcgattatca ggtccattaa gaatactaga taatatacca atattatctg ataaaaataa
    12421 atcatctttt gtcaaaatct gttctttacc acctatacca taaaaatgtt cacttccatt
    12481 tgctaattct attttcaaag gtaattctat tttatcaagg tcatgacctg ctgttagaag
    12541 aagatttttc aattctgcta aaaacattga ctcaattgtt acatctacat ctgggatacc
    12601 ttttgatttt aatagaatag attctaattg aagcaataca gggtaagttt tcttgaattt
    12661 tttataatac tttatataac ttgaagcagg ctctgtttta attttttctt tacgattgta
    12721 ttctggatgt tgttctataa tatttttaat tgtagaatct ttaagaatta aaaaattttc
    12781 tttattaggt tttgaatgtg taatatttat aaccataact ccaaatttaa tattgggata
    12841 aatttcttta actttatgtg atatttctaa cataatatag actccttttt attttttatt
    12901 ttatgatata atcttagcaa gttaaaagtg gattttgtag ttccattttt atagtaattt
    12961 tatagaacca cttttggagg tattagaatg tggattacga ttaatagaaa tagcaatata
    13021 tcattatcaa gacaaattta ttgtgagata aaagatatga ttctagatgg aagcttaaag
    13081 tttgagcaaa aattgccatc atctaggact ttatcaaaag aactatctgt atctcgtaat
    13141 acagttttag atgcatataa ccaattaatt gcagaaggat atttggaaac aagaaatgga
    13201 tatggaacaa ttgtagctaa aggaatatca gggcatcaag ttgattttaa gttaaattat
    13261 acttttagaa atgaaaaaga tttacatact aataaagaaa aaaattatat agattttcgt
    13321 tcaggagttc cagaattaaa actatttcca cgcaaagaac tttcaaagct atataaacaa
    13381 atactgtatg atttatcaga ttctgatttc agatatggaa gtacagctgg tgtatgggtt
    13441 ttaagagtgg aaatatctaa gtatttattt cgtactagag gaattagatg taatcctaaa
    13501 aacattatga ttgtttctgg ttcaacacaa ggtttatctt taatttcaca acttttatac
    13561 aaaagtaata aaaaggttgc tgtagaagac cctattcata aaggcttatt aaaggttatt
    13621 tcatctattg gatattcggt tgttggaatt aaagtagatg ataagggaat gaataccaac
    13681 ttaataaaat caataaaggg gatttctttt gtttatacta caccttctca tcaatatcca
    13741 ttaggaggga ttttacctat tcaaagaaga ctagagctta ttaaatgtgc aacagagaat
    13801 gattactata taattgaaga tgattatgat agtgaattta gatatgaggg tcaaccaatt
    13861 agttctcttt atgaacttaa cccgaataaa gtaatttaca taggttcttt tagtaaaata
    13921 ttatctccag ctattcgtgt tggatttatg ttattaccag acaatatttt agaaagctat
    13981 aaaaccttaa aaatgtactc tgatgttcat acagaaacaa tattacaaca tgtattagct
    14041 gaatttattg gaaatggagg tttagagaag tacatattta gaatgaagaa gttgtataac
    14101 agaaaacgta agtacctcat aaaagtttta aacacagaat ttaaaaatga atttaaaata
    14161 aaaggtgaag cagcaggact tcatatagtt gctcatttct ataatgttaa atttacggaa
    14221 agtttaattc ataaaatttt tgaagcaggt ataagagttt atcctataca acaatgtgca
    14281 attgagaaca tattggaata tggccatgaa attatactag gttatgctca tcttagtttt
    14341 gatgaaattg aaaaaggtgt aagaatactc agcaaaataa tacatatgaa tcaaggtaca
    14401 tagataaaac atgtagcaat ctacatataa tagatgcaat taagtttaaa ctttaaaaga
    14461 aatatatatt aatattcttg tataaaaata cctgatattg tgttatcgat actttgttgc
    14521 tatacactaa attaagatag caatttatat taagaaaaaa ctttttgcat aagttataat
    14581 acttaaaagg agaggataga gttgaaaaca aatacagatg ttattatgga gataattgat
    14641 tatatagaag aaaatcttac agaaaatcta aatttagata aaatagctga tattgctaat
    14701 tattcaaaat accatttaca cagaatgttt acaaatgttg taggatgtac tatacatcaa
    14761 tacatacaga ggaggagact tactgaagca gctagacaac ttatatttac agataaatat
    14821 attatagaca tagctattac agtaggatat tctacacatc aatcttttac actagcattt
    14881 agaaatttat ataaagaatc tccacaagta tatagaaata aagatgaatt ttatcctatt
    14941 caacagagat ttgttttaaa tctaaatcat caaattcaaa gcaaaaaatg tatttcttat
    15001 gcaaatatga attataaaca aagaagagat gaagtaaatt cttatcttaa ctttaatatt
    15061 aattgtgagg tggaagtagc atgagtttag taccacatgt aattgaacaa acaggtcaag
    15121 gggaaaggtc ttatgatata tattcaaaat tattaaaaga tagaattata tttataggag
    15181 aagaaattaa tgatgctatt gcaagtttag tggtagctca attattgttt ttagaatcag
    15241 aagacccaga ttcagatata attatatata taaatagccc tggtggatct actacagcag
    15301 ggtttgctat atatgatact atgaattatg taagatgtga tgtatcgact atttgtattg
    15361 gaatggctgc aagtatgagt gcttttttac ttgcaggagg aacacgtgga aaaaggtgtg
    15421 ctttacccaa ttcagagatt atgattcatc aaccaatggg aggagctaaa ggtcaagcaa
    15481 cagatgtaaa aatagcagta gataatatat tgaaaataaa agaacaatta gataggattc
    15541 tttctgaaaa tacaggtaaa tcaatagaag atattagaag agatacagat agagataatt
    15601 ttatgactgc aatagaggct aaagagtatg ggctgattga ttatataatg aatagaaatg
    15661 aataaagtat caaagtatca tataaaaatg tctgctttac aaatatatag agaatattaa
    15721 atgagagtgt ctcaaaatga actttttagt tcatgggaca ctcttttttg tatgaaatta
    15781 aatatgtttt tattatttca acaattatta tcaaatttca agtttaataa attctaatat
    15841 aaaaaataca taaaagataa gactctttag caactttttg tcgttaaaag tttttataaa
    15901 tttaataaca aaataagata aagtaccata tattacttat gtctgcaata tatagaagac
    15961 aaaggagttg tacattatga ttacaataag tttgtgcatg attgttaaga atgaggaaga
    16021 tgttatagga agatgcttag aatgtgtaaa ggatatagta gacgaaatta tagttgtaga
    16081 tactggttct acagattcca caaaagaaat agtctcaaaa tatacagaca tggtgtacga
    16141 tttcgaatgg atagatgatt tctctgctgc aagaaatttt tcattttcta aagcttctaa
    16201 agactatata atgtggcttg atgcagatga cataatttta gaagatgata gagaaaaatt
    16261 attaaaagtt aaagaaactt tggatacttc cattgatatt gttatggcaa aatacaatgt
    16321 atcctttgat gacaatggta aaccaactct ctcatattat agagaacgat tgtttaaaag
    16381 aagttctaat cataaatggg ttgaccctat tcacgaagta atacctcttt atggagaaat
    16441 attttattct gaaattgcaa tatctcataa gaaactccat agacaggacc ctctaagaaa
    16501 ccttaggatt tttgaaaaaa tgatttcaga aggaaaagta ttggggccaa gacaccaatt
    16561 ctattactct agagagttgt actataatgc taggtatgaa gaagcaatag aaggttttac
    16621 taagttttta gactcatcaa gaggatggct tgaggattgt atcagtgctt gcagagattt
    16681 agccacatgt tattacttaa ttaatgatga aaagagtgca ttatattcat tgtttagaag
    16741 tttcgaattt gatgagccaa gagcagaagt ttgttgtgac ataggaaagc atatgtttga
    16801 tagacaaaaa tataaagaag ccattttttg gtataaggta gcattaacaa gagataagaa
    16861 tgatactaat ggaggcttta agtcaaatga ttgttatgga tatataccat ctatacaatt
    16921 aagtgtctgt tatgacagat taggagaatc agataaagct atttattatc atgaaaaaac
    16981 aaaagaaatc aaacctaacg atagtgctgt tttgcacaat gaaaattatt ttagtaaatt
    17041 taaaaatagt tgataggtca caatcatact aaatttgaca tattatatat agagcttaaa
    17101 agctcaaata tatcaggagg tgcttttaat aatgagtaga aataaatatt ttggaccatt
    17161 tgatgataat gattacaaca atggctatga taaatatgat gattgtaaca gtggtcgtga
    17221 tgattataat agctgtgatt gccatcattg ctgtccacca tcatgtgtag gtccaacagg
    17281 tccaatgggt ccaagaggta gaacaggccc aacaggccca acaggtccaa caggtccagg
    17341 tgtaggggca acaggcccaa caggcccaac aggtccaacg ggtccgacag gtccaacagg
    17401 aaatacagga ggaactggaa caggaggccc aacaggacca acaggtccaa cgggtccaac
    17461 aggtccaaca ggaaatacag gaggaactgg aacaggaggc ccaacaggac caacaggtcc
    17521 gacaggtcca acaggaaata caggagcaac aggatttgga gtaacaggcc caacaggcgt
    17581 aacaggagca acaggagcaa caggagcaga tggagtaaca ggagcaacag gcccaacagg
    17641 agcaacagga gcagatggag caacaggacc aacaggagca acaggagcaa caggatttgg
    17701 agtgacaggt ccaacaggcc caacaggccc aacaggagta ggagtaacag gagcaacagg
    17761 attaataggt ccaacaggag caacaggaac acctggagcg acaggcccaa caggggcagt
    17821 aggagcaaca ggaataggaa taacaggtcc aacaggtcca acaggagcaa caggagcaga
    17881 cggagcaaca ggagtaacag gtccaacagg agcaacaggg gcaacaggag cagatggagt
    17941 aacaggtcca acaggggcaa caggagcaac aggatttgga gtgacaggcc caacaggtcc
    18001 aacaggagca acaggaatag gaataacagg agcaacaggg ttaataggac caacaggagc
    18061 aacgggggca acaggagcaa caggcccaac aggagtaaca ggggcaacag gagcagcagg
    18121 actaatagga ccaacagggg caacaggagc aaccggagca gatggagcaa ccggagcgac
    18181 aggggcaaca ggagcaacag gtccaacagg agcagatgga ttagtgggtc caacaggagc
    18241 aacaggagca acaggagcag atggattagt aggcccaaca ggtccaacag gggcaacagg
    18301 agtaggaata acaggagcaa caggagcaac aggagcgaca ggtccaacag gggcagatgg
    18361 attagtaggt ccaacaggag cgacgggagc aacaggagca gatggagtag caggtccaac
    18421 aggagcaaca ggggcaacag gaaatacagg agcagatgga gcaacaggtc caacaggggc
    18481 aacaggtcca acaggagcag acggattagt aggtccaaca ggagcaacag gagcaacagg
    18541 attagcagga gcaacaggag caacaggccc aataggagca acaggtccaa caggagcaga
    18601 tggagcaaca ggggcaacag gagcaacagg tccaacaggg gcagatggat tagtaggtcc
    18661 aacaggagca acgggagcaa caggagcaac aggagcgacc ggagcaacag gtccaacagg
    18721 agcaacaggc ccaacaggtg ctagtgcaat aatacctttt gcatcaggta taccactatc
    18781 acttacaact atagctggag gattagtagg tacaccaggt tttgttggct ttggtagttc
    18841 agctccagga ttaagtatag ttggtggagt aatagacctt acaaacgcag caggaacatt
    18901 gactaacttt gcattttcaa tgccaagagc tggaacaata acatctattt cagcatactt
    18961 cagtacaaca gcagcacttt cacttgttgg ctcaacagtt acaattacag caacacttta
    19021 tcaatctact gcaccaaata actcatttac agctgtacca ggagcgacag ttacactagc
    19081 tccaccactt acaggaatat tatcagttgg ttcaatttct agtggaattg taacaggatt
    19141 aaatatagca gcaacagcag aaactcgatt cttactagta tttactgcaa cagcgtcagg
    19201 tctttcatta gttaatactg tagcaggata tgcaagtgca ggaattgcaa taaattagat
    19261 taaataatta aaaaaggctt ctagtatcct attgatatta gaagcttttt taagactgat
    19321 tgataaacat agttttaata ttaattagta cacatattat aaaatactat tatataatat
    19381 aaaataataa cattaaattg gaaatataga attatctagg ggtgatacta tgaaaaataa
    19441 gattgaaaca ttaatgaaca actttgtaaa aaattatcat aaatcaaata atataactac
    19501 aaagtggcaa gaacttttat ttgcttatgc ctctgctgaa gatgatatgt ttcagaagct
    19561 aaaaagtgtt gtaggaacat ctcatgttat gccaaaagat tttctgaaaa atgggaaaac
    19621 tataattaca tattttatac cttttgatga aagtgtaaat aatagtaata ttggagggtt
    19681 tgaatgttct gaaatatggg caagagcgta catagaaact aatatgttga tttcaaattt
    19741 aaatgatttt ttatgtgaag agctagagaa gataggggct aattcatatg ctattcctgg
    19801 gtcgtataat tttgacagtg aaaaattggt cagtgattgg tcacaaagac atatagcatt
    19861 catagcaggt cttggaactt ttggtttaaa taatatgctt attacagaaa aagggtgttg
    19921 tggaagaata ggaagcataa tcacaagttt agaattagag ccgactaaaa gaccagaaaa
    19981 tgaatactgc ttgtataaac ataataatag ttgcaaaaaa tgtgtcaata aatgcgtaaa
    20041 agaagcttta aaagaagatg gatttaatag aaataagtgt tatgaaatgt gtatgataaa
    20101 tgaaaaaaaa tacacttata taggtggatg tgatatttgt ggaaagtgta tagttggagt
    20161 tccttgttca gtaataaacc catcaagata atggatttaa gttgactaat aatttaaaat
    20221 atagtttata atgaaaaaat tttagttatc aaaataaata taatataaaa actaaataaa
    20281 tgtgtttaaa aaatggataa ataatttgtg ttgattgaca accagttata ctgttgatag
    20341 acttatatgt atcgctgtga tatataagga gggttgagat tttatgtttg atttaatctt
    20401 tatttttgca tagcaagggc tattgcaact atgaaattta atatataaat agccctttgc
    20461 tattcctaat gaaaaattac tatttaatag gagagcaaaa tgagctataa aaaagcaata
    20521 catgtattac cagaagaatt acttgaatta atacaggaat atgtagatgg tgaatgtatc
    20581 tatattccta gaaaatctaa taataaaaaa gaatggggaa gtaatacccc tactcgtgaa
    20641 gagttggcaa ttagaaatat gcagatttac gaagactatc aagttggata tgatttgcaa
    20701 tatttatcgg aaaagtatta tttatctttg aaaagtatcc aaagaattgt attacaagaa
    20761 aaaagaaaag atatctaagg atgagccagt atagttttat aattatactg gctcattttc
    20821 ttttgataaa agtgtttgag gtatagtttc ttttaagggg atgatataat ttaagtaatt
    20881 tcaatgactt atacagtagc taacaaagtt ggttgtaaag tacctagtaa gaagtaatag
    20941 cttattatat gcttgaaatt aaaatttatt aaaattagga ggtcttgttt tgaaaaaaat
    21001 gcttgattat attccacctt ttcataactt tctattagtt tagcaatctt atagaaagga
    21061 aattgtattt atgcaaaaaa ttacttggga ggtggaatat atctcccagt tacctgtaat
    21121 taggtattgt aagaaatgtg gaaaaaagac tgaatatatt tgttcaggtt tatttaggat
    21181 taatgctcaa cataaatatt tggatatatg gttaatatat aagtgttcaa actgtgattc
    21241 aacatggaac tcaactatat attctcgtgt caatccaaaa agcttaaatc cagaaacact
    21301 agaacaattc catacaaacg attataatct tgctaagcaa tatgctatga atatggaact
    21361 tttacataga aatggagcag aagttggtat tccagagtat aagatagtag gacaagaaat
    21421 taacattaat gaccttacac aagttcatat tattagtaaa tatccatctc atttaaaggt
    21481 gtcaacatta ttacgtgaga aactaggttt atctaggaag gtatttgacg aaatgttaga
    21541 ttgtggaatt attagaagtg aatcaggagt tgatttaaag aaaggtaaat taaattatga
    21601 aattatttta gatataggca aggagaagca aaagtcttca aattgattag aataactaac
    21661 ttaatatatt ttcaggaaca tcattttaaa aaaatagtga ctttaaaaaa tttaaagcca
    21721 ctatttttta atatgtttta tctatataac tattattttt ctaggttttt taatatttcg
    21781 taatagtgtg ttgtggctat ttcacttgat tcgccttttt tacaaaaaga taatttagat
    21841 agttttggaa ctacacgttc aacgacaatt ttattaccta tcattttaga ttcaagtatc
    21901 ttcaatgctt ctaaaaacct cttatccttt tttgcacgat tataaaatga taaaacgtag
    21961 acataaagga ataggttata attacggaaa ggatattcaa cttgcataaa taaggttcca
    22021 attccatagt gacaagggcc gattggtttt ttaatagtcc aatggtcaag tagaaaatct
    22081 acagctttgt caagttttga ttctttattt agataattac taaaacggaa tgcatttaat
    22141 atattaagag ttggtagtgg atttgagtat tcagtttctg gacctcgacc aaaactaaat
    22201 ttattgcaac gccacccacc atcagtatac tgtgtatcta aaaaatgttg aaacgttttt
    22261 tgcattctaa tgtcagatgc atatcccata tgacagagta cgttagcagc atttgcagta
    22321 tgacaagggt aaatacctcc atttggatat agtttaaaac gtccatcttc tcgccatgtg
    22381 ctaaatatca agtcagcaca ttctttaagt aaagtatcat cagggttcac acctaattct
    22441 aatagataaa gtacactatc aagagtagag aaaggagaac cttttattaa acgcttatca
    22501 ggtgtagtcc aatagtctgc accattatca tacctgtgtg ataatattat ttcaatatct
    22561 gataaagatt gattttctac attcattttt taccccattt catattatat tctattaaat
    22621 ctatcaaaaa ctttattata atattattta tatttataat taatttattt tatttaaaat
    22681 tgcatctata gctgtatcag gaacaactgc taaatcttct gcattgaagt aataaactat
    22741 attagaatca aataatatat ttgaatttgg aggaaattca tcatctccaa accaaagtat
    22801 aaataccata gatgtattac ccatatatgt aaacctataa gctaaatctc ccatgtttac
    22861 tttttcagct ccaattttct ccataactaa cttatatttg tctatttgag atgtaaaaac
    22921 ttctgaaagc ctatatagag tcctgtttaa aaaatttgga taataaacat tgcctcctgg
    22981 tatttcttta aaagttacat atttattagt tctaggaata cctttagcat tgattaaata
    23041 tctaactatc aagattttaa gctcaaagtt attgcaaagg ctaccattac tattccaaac
    23101 ttctccactt ggatagtcta taatatattg tctatcaaaa aaattgagtg tgaatctatt
    23161 taaatcatta ttgaaaggta tattacttaa attactaatt gtatgagggt ctaattcttt
    23221 gagttttttt cgaacatagt caaatggaac attataatt
//
LOCUS       DAEPXF010000051        22801 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain HB27
            SAMN13376389-rid12479423.denovo.055, whole genome shotgun sequence.
ACCESSION   DAEPXF010000051 DAEPXF010000000
VERSION     DAEPXF010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13376389
            Sequence Read Archive: SRR10518432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 22801)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22801)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001069624.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001069624.1
            Genome Coverage       :: 315x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/15/2021 01:54:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,165
            CDSs (total)                      :: 4,076
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 89
            rRNAs                             :: 10, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 10 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 19 of 69
            Pseudo Genes (incomplete)         :: 44 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 10 of 69
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22801
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13376389-rid12479423.denovo.055"
                     /strain="HB27"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="China: Hebei"
                     /collection_date="2017"
     gene            101..2029
                     /locus_tag="KRL67_001698"
     CDS             101..2029
                     /locus_tag="KRL67_001698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide transporter, OPT family"
                     /protein_id="HBH1522581.1"
                     /translation="MNKKLPKGAYGEVSGKDYVPYITDKSRTGGNVAVLIIGIILAAI
                     FAASTTYSGMKAGLTVAAGIPGAIIGSAFVGAFARSKGILGKNLIQGMSSGGESVASG
                     FIFVLPAVILIGSQITFLEGLAVGVGGVLFGIGIAAIVHNYLIVEEHGKLMYPESMAI
                     SETLVASEAGGDSIKYMGIGFVISGFITVLTGSFLNVANNVMSLVGSKFYKWKFDIEV
                     NPLLLGIGFIVGLEVSLTMFAGSILSNFGIAPLIGYFTDMAKDGAMVWNNPAMPLNQM
                     DVGAISSSYVKYIGAGMMLCGGIIGAIKLIPTIIASIKETLKAKSSTGEGEEGSSIQM
                     ILLLGGVVIGFLAAFLISGNIVMAIIGAIISLLLSLLFVIVAGRLTGTIGTSNLPVSG
                     MTIASLVIVTLVFVIMGWTDLEANKSLLLFGSFIVVAIAIAGGYTQSQKVTYIIGGSK
                     NEMQRYFTIASIVGVIVVVGVILLLSDQLRATGDNVQFALPQANLMSTLTSGIMSGSL
                     PWVMIIVGVFMAIVLYALNLPIMTIAIGFYLPIATTSIILVGALIRLFVELVSKTEKE
                     KEVKVSNGISLSSGLVAGGSIIGLIGIILQVTGVVTPKVPSGFAATNSMAIALLVVLV
                     VLTALPIVLSKVKNNEQE"
     gene            2016..2363
                     /locus_tag="KRL67_001699"
     CDS             2016..2363
                     /locus_tag="KRL67_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PqqD family protein"
                     /protein_id="HBH1522582.1"
                     /translation="MSKSNDVLDIVFKITDELEYEVAENSVVTILEKQDHKIQKFFRK
                     LKARIPKYKKIELDEYSSFVFLQIDGQRTVREIGKNLEAKYGEESHPLYERLLVFLNH
                     IEVNCHYIERLDI"
     gene            complement(2611..4530)
                     /locus_tag="KRL67_001700"
     CDS             complement(2611..4530)
                     /locus_tag="KRL67_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_071223118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HBH1522583.1"
                     /translation="MNLMTLENISKSYSEKKLLENISLGINEGEKIGLIGVNGTGKST
                     LLKIIAGVEESETGNITKTNGIRIEYLPQNPTYNENSTVLEQVFNGTSEELKLLGEYQ
                     EILEKLNSNFTEELNKKLLSVHEKIDALNLWDLESEAKSVLTKLGINDFNQKIAELSG
                     GQRKRVSLASALITPCEFLILDEPTNHLDNDTIDWLEEYLNSRKGSLLMITHDRYFLD
                     RVTNRIIELDKGRLFSYDGNYSIFLEKKMERLSLEASIEDKRQNLIRTELAWVRRGAQ
                     ARSTKQKARLQRFDELINKDSFKPDENLDISVASSRLGNKIIEIHNISKSFEENTVID
                     NLEYTLTRTDRIGIIGKNGMGKSTLINILNGEIEPDSGYISIGETVKIGCFSQDDSHM
                     NIDMRAIDYVKEASDYIETSDGTRITASQMCERFLFNGTMQYTLIGKLSGGERRRLHL
                     LKILMTAPNVLLLDEPTNDLDIETLKVLEEYLDEFKGVVVTVSHDRYFLDRICNKIFA
                     YEGNGKIHIYTGNYSDYLIYREIQGIEFEESKRESVKKDDSTTPKKEKPKNDKKLKFS
                     YNEQREFENIDSDIEKLESKIATLDESTSKFSTDFTKLQEILDEKSKLEKELEYKYER
                     WEYLNNLADEIANNK"
     gene            complement(4556..5362)
                     /locus_tag="KRL67_001701"
     CDS             complement(4556..5362)
                     /locus_tag="KRL67_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HBH1522584.1"
                     /translation="MNLILGDNMSKKSKRRKINKLKLTIVITVLFLALLSIYEILFSN
                     PNKKDNIKSQETMQSKNNTTDDSKNNQSSTKKQEDNNIKPTFSYSSIPDNIKNKMIGK
                     SMPTDEPISFDSLSYLKLTYYGFDEKTHQGEMIVNSELAPEVVDIFKELYEKKYPIEK
                     IKLIDDYDAVDEKSMSDNNTSSFCYRTIAGTNVVSNHGKGRAIDINPLQNPQVSGNDV
                     TPKVSTVYADRSSTKFGMIKKGDDCYNAFVSRGWSWGGYWKNPDYQHFEK"
     gene            complement(5421..6869)
                     /gene="ddlR"
                     /locus_tag="KRL67_001702"
     CDS             complement(5421..6869)
                     /gene="ddlR"
                     /locus_tag="KRL67_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator DdlR"
                     /protein_id="HBH1522585.1"
                     /translation="MIFSNLKLNDNEPIYIQLKNYISDMISKGLIPDNSKLPSTRELS
                     QLLQVSRNSVVLTYEELKSEGLIYSISGKGTFVKSKNKSSNTTWSLNWDCLENTYSKK
                     ANELDIIKSEIPWSSDLISFKSISPDGDLFDMEELKKSFLNRISLEGHKLLNYGYAQG
                     YKPLIDYLLEYMTNKGVDISNKDILITNGFTEGFDIIMSSFTQEKDYIICENPTHNTA
                     IKIMKSHNLNILGVDINEDGLDFNMLEDNLVKYKNKIKFAYITPSYHNPTGIVMTPEN
                     RYKFYNLMKKYNIAIIEDGFNEELLYNSSHIFPICSLDNMNNGVLYIGSFSKILFPGM
                     RIGWIFADKKVINKLESVKRCRNIHVSFLDQGILFDYLSNGGFEKYIKKIRKFYGDKF
                     NFAYKCVKKYIKTEYILGDGGLHIFIKLKNIDTRVLLEKCYEKNVIFMPGDLFFTDNS
                     GFDTLRLGFSRLSFEDIEIGIKIIGETINEMF"
     gene            complement(6893..7792)
                     /locus_tag="KRL67_001703"
     CDS             complement(6893..7792)
                     /locus_tag="KRL67_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HBH1522586.1"
                     /translation="MKIAVIMGGISSEREVSLNSGKEIYNNLDKNKYEVVKVIIDDKK
                     DIFTKIPEDIDFAILALHGKFGEDGCIQSILETMDIPYSGCGPLCSGMCMDKNITKKM
                     LRDSNLPTAPWVLVKSVDEIDYDEIDNIGYPVFIKPNSGGSSVATFFIHSKDEVEEAV
                     RKGLEVDEFVMIEKYIPGGEYTSFILNGEVFPTISIKSDSGFFDYEAKYSVEKGAKEE
                     VVYLDEELQKRVNEISETCWKIFNCKAYVRVDMIISEGIPYVLELNTLPGMTQTSLIP
                     RSAAARGIKYSELLDKLIEYSLN"
     gene            8050..9306
                     /locus_tag="KRL67_001704"
     CDS             8050..9306
                     /locus_tag="KRL67_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011591286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HBH1522587.1"
                     /translation="MKAEIISVGTEILLGDIVNTNSQFLAKELASLGIEVYHQSTVGD
                     NKQRLLECFDESLKRSDFVITTGGLGPTGDDMTKETAAEYFGQKLELHKPSLEVLESF
                     FVKTGKKMAENNMKQVYFPKDAIVLKNNNGTAPGAILKKNGKFIIVLPGPPREMKAMF
                     NESVKPYLQQFTNEMLVSKTLRLYGIGESNLELEILDIINEQTNPTVALYAKELEVTI
                     RITAKAENEREAFKLIKPVEEKIKIRVGKYVYTEGDISISEGETALEDAVSKLLVEKN
                     LTIAVAESCTGGLVSSSLINYPGISSVFLEGCVTYSNDSKMKRLGVKRETLEEFGAVS
                     EQTAIEMAEGVAKGLKANIGISTTGVAGPGGGTKEKPVGLVYTAIYINGKTIVKKNIF
                     NGDRRKIRLRATRDLLNELRIQLEKL"
     gene            9376..10155
                     /locus_tag="KRL67_001705"
     CDS             9376..10155
                     /locus_tag="KRL67_001705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HBH1522588.1"
                     /translation="MIKHIFCDLDGTLYENGTITKEDIIAIEEIEKKGVQFNVATGRI
                     FKQARNIIKDSLDMNGYYVCENGSFIYDKDYNVIFKRTIDDNLVKKVIDRFESSDAQL
                     YFKYKGDVIVGSDTTAFRHYSSDFIVDPDFEKRSSFDNLIGNIGVCSENLEELSRIEL
                     YLKSEFSEVLEIYFSGTYTLNIVPKSVSKRGSIEHVIKTLNVSPDEVATIGDSPNDIC
                     MLEGFKYSFAMSKARGDVKQSANYVVDSVKSAIDVIMEINS"
     gene            complement(10249..10833)
                     /locus_tag="KRL67_001706"
     CDS             complement(10249..10833)
                     /locus_tag="KRL67_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc dependent phospholipase C family protein"
                     /protein_id="HBH1522589.1"
                     /translation="MLMNTHFIMAKSILDNIDENKTFFISEKNFIYGNIKPDAFSKYK
                     LKKHYLDESFNMIVSKINYLCSLNIDSLSKYFSVSRLSQELGVICHFLCDFFCVAHSE
                     RWEFKHSLNKHVTYERELGAFAKDVDLSKIKCGTINSSFEKFFTKLYSDYKKRTDYMN
                     DLKFSAYTCNSVINYILDSILSNTIKSYNIANCG"
     gene            complement(11033..12463)
                     /locus_tag="KRL67_001707"
     CDS             complement(11033..12463)
                     /locus_tag="KRL67_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBH1522590.1"
                     /translation="MELDLEFYQKILEASHDEICVSDDKGIIIYCNKAFEENYGLKKE
                     DILGKNVSFLEDSGYSTKSPIPIVLKTKTKFSLEQDTQAGKKLIITATPIFDENGNLE
                     FTVENCRDITELNNIKNKLEDTKKQVKKYKSEVETLYRTALRIEDTVIMDGIVMRPII
                     NTVNHVSKTDVSVLLLGESGTGKSSLARYIHHNSNRSNGPFITINCATISPQLLESEL
                     FGYTSGAFTGASTKGKVGLVELANGGTLFLDEIGDIPQNLQAKFLQLIQDRTFTPVGS
                     LKNKKVDIRIISATNADLVSKVKEKKFREDLYYRLNVIEIKLPPLRERRDNLVEIIKY
                     YFNRYSSDFNLNKTISKEAMDAIANYGFPGNIRELQNIIQKILLTCTDNHITIDDLPN
                     ILTKNIHITNNGNKTHISQINKVIISDSKSTNYKNKNFDTLIKEYEKNIILDAYEKFG
                     SSYKVAKHLEISQSKANRLIRKYTNT"
     gene            12791..13735
                     /locus_tag="KRL67_001708"
     CDS             12791..13735
                     /locus_tag="KRL67_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HBH1522591.1"
                     /translation="MGEKSKGYVFIAIAGLLWATLGLFGKFLMGNGLTSEQVAFTRLF
                     FGFIVLGVYSSIRTPQILKISKKGIIYSVIIGIICQAMFNLCYFKAIDIAGVSIAAVL
                     LYTSPLFLAIFSKVCYKENITRSKLFSLILCFIGAIMAVSGGRLDFQGLNAFGLFLGT
                     LSAIAYALMPTISKNALKEFSSSTILVYSFLFGAIFMIPSSRPWEILNYAKDLDVLSC
                     MLMLGIVPAALAYIFYATGISKGVELSVAGVVASVELVGSVIIGCTILGESFSLGKLF
                     GVMLMLISAVVALNLSYDEIRIFYKSNKLKQIEKTESI"
     gene            complement(14006..14941)
                     /locus_tag="KRL67_001709"
     CDS             complement(14006..14941)
                     /locus_tag="KRL67_001709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893027.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HBH1522592.1"
                     /translation="MDKFKYAFNNKRYHTWNYYLRNTFGEKVFKVSINAGFTCPNIDG
                     SLGYGGCTYCSKEGSGDFAGNPKDNLISQFYNIKEMMLKKWPHAKYIGYFQAYTNTYA
                     PLEVLKEKYETILELEDVIGLSISTRPDCLPDDVVEYLSELNKRTNLWVELGLQTIHD
                     STSKIINRGHDYKTFVEGVEKLKSKNIKVVVHIINGLPGEDYNMMMETAKAVGKMGVD
                     GIKIHLLHVIKDTPMEKMLQNGMLTLMEQDEYINLVCDQLEILPETMIIHRLTGDGKR
                     DELVGPIWSLKKWEILNQIDDTLKARNSYQSCKFV"
     gene            complement(15061..15654)
                     /locus_tag="KRL67_001710"
     CDS             complement(15061..15654)
                     /locus_tag="KRL67_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF308 domain-containing protein"
                     /protein_id="HBH1522593.1"
                     /translation="MNFCYNCSIDFGGDFMYTNFNIDFNNFNKKENATKFMLMGVLLI
                     ILGLLCLTFKTLGIKLISWTFGIALLFFAYLNLKNINELKRYATKEEIKPSINIQWVL
                     IIACILLFVFPQKIQSIFSLFLGFYLIFNQLVALVNSKNNPYSKFTTWNIVKILFGIC
                     LILSPLFLSRFIVSIMSFFIILFGLVLFFSGNTARKY"
     gene            15761..16189
                     /locus_tag="KRL67_001711"
     CDS             15761..16189
                     /locus_tag="KRL67_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HBH1522594.1"
                     /translation="MTKLWAIIFALLAGGSTALEAFINGELGKSTTALVATFISLTVG
                     AIFFLASIIVTGDLKCLVSINGFNYKLLLGGVFGGLIIYFTVKAIPNLGVSNTLILTL
                     VSQVLVGFFIDSVILGQETMHLYKYIGVILLILGTFFIVN"
     gene            16256..16924
                     /locus_tag="KRL67_001712"
     CDS             16256..16924
                     /locus_tag="KRL67_001712"
                     /EC_number="6.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diphthine--ammonia ligase"
                     /protein_id="HBH1522595.1"
                     /translation="MKEVLKLADEKFVMSFSGGKDSILALNRMLKKGYKPVALLTTIS
                     EEHGKSWTHNLEYNMLKQVSSNIGLPLLVAECGVEGYEESFERALIKAKNMGATICAY
                     GDIDIESHRKWDTDRCEAVGMKVDLPLWQESREDLVYEFIDSGFCSVVTKVNLKHLGE
                     EFLGKKLTRELVEKIKNAGADPCGEHGEYHTFVVDGPIFKKPVEYEVKGTLIKDGYGY
                     LEIK"
     gene            complement(17042..18397)
                     /locus_tag="KRL67_001713"
     CDS             complement(17042..18397)
                     /locus_tag="KRL67_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HBH1522596.1"
                     /translation="MDTKISINENMSLGKRFFKYLAPSVVAMWVFSLYTMVDGIFVSK
                     GVGELALAAVNISMPFINFIFAVSLLFSTGASTIIAIYLGKKDIKSANEVFSFNLVSI
                     IILSIIILAITFFNLDRLALFLGATESTIGMVKDYLGIIIFFNGFFIVSYSLEVIIKT
                     DGFPILATVGVIISALTNIILDYLFVIEFGWGVKGAGIATGLSQVFSTIFFLIHFLRK
                     NSTLNFSKFRIDFKTLRKIVFIGFPDSTTELSCGIVVLLFNLSLTKYIGENALIYYSV
                     INYINTLVLMTMMGITQGMQPLTSFYYGAGNIDNVKKLLKMGIKATIIASIAVFAICM
                     AFSEPIVSLFIHPEETMLFNEGVRVFKIFSISFLLVGINVIISGFFVSVEKPSISTVI
                     SLGRGLVIVVLSLISMILIFGGQGIWMTTIVSEFICLILSLVFLKKNFSTLDSNLNKV
                     A"
     gene            complement(18901..19395)
                     /locus_tag="KRL67_001714"
     CDS             complement(18901..19395)
                     /locus_tag="KRL67_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HBH1522597.1"
                     /translation="MLNSAKNQVGFIKSVLKESYRLKEISENICDFVTTLIKKNSSTS
                     KFYTKQKAQLLLEKSKNIPIGIPIFLMLTLGLRFGEAVSLIWSDVDLDKKIIHVNQTL
                     VYVDNKIIFKDPKTPKSKRKLFAPDELISLLEEEKFKQNKLKLQAILKNEFDLICLNK
                     RFNP"
     gene            19491..19847
                     /locus_tag="KRL67_001715"
     CDS             19491..19847
                     /locus_tag="KRL67_001715"
                     /inference="COORDINATES: protein motif:HMM:NF012870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HBH1522598.1"
                     /translation="MVFETFDKVIELYPNAKPVFHSDRGFQYTSKVFESKLLAQGMIQ
                     SMSRAGRCIDNAPMEGIWGILKVEMYYLRKFDTYEQLVKIIENYIYYYNNFRYQKRLN
                     SMSPLEFRKYLSTEIA"
     gene            complement(20926..22659)
                     /locus_tag="KRL67_001716"
     CDS             complement(20926..22659)
                     /locus_tag="KRL67_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HBH1522599.1"
                     /translation="MSILLKKAPKLAKHIITSFYINRDIDEVLKYLCENVTWIGPGEQ
                     EFLTSFNEIKNYFYAGQDEIPSCDINNDIFETVSEDENRCMVLGKYTVRTKENAQMIL
                     EVNQRCTFEIIEDRGKLLVKHMHISNPYGEMQLDEYFPTKIGTQSYDYLQRLLKEKTE
                     VIEMITNNINGGLKGSNDDSTYSFFYVNEGLPKILGYTYDEFMEMSGGSAVGAVYPPD
                     LPKALEDCERCFAKGPTYSSEYRIRKKDGTLMWVLDSGMKSLNSDGIVKINSIITDIT
                     QLKNIESELKLERERYRIALQNITDIMFEYDIENDTLIKYQRVEIDKKIELENFETKN
                     YSKLLEFGKIIHLDDIGKLLEVLHGNLRETIEIRQINSLTKNEWRWIRVQCSVIYDSD
                     HNPIKTIGVLKDITEDKSKLESLINQAQRDPLTQLYNQRVSQNLIQEYLCSSDSKNND
                     ALLVIDIDDFKTVNDTFGHLEGNEVLVAVSKILLHNTHDKDIVARIGGDEFTIFIKSL
                     TKDLIIKITNDILNDASKIKVKDNHKITLSIGIAFTDDSTKLYKDLFSKADKALYLSK
                     ADGKNCYSTYE"
BASE COUNT         7932 a         3028 c         3273 g         8568 t
ORIGIN      
        1 ttttagtagt tttgactcgc tatttgtaaa ataataaaaa gttaagtttt atatgtatat
       61 atgttataat atatacaaat tgaaaagggg tgaaaatttt atgaacaaaa aattacctaa
      121 gggtgcttat ggtgaggtta gtggaaaaga ttacgttcca tatatcacag ataagtccag
      181 aaccggtgga aatgtagctg tactgattat cggtattatt ttagctgcca tttttgccgc
      241 atctacaact tactctggaa tgaaagccgg tcttacagtt gccgcaggta ttccaggagc
      301 tataataggt tctgcttttg ttggagcatt tgctcgttca aaaggtattc ttggtaaaaa
      361 cttaatccaa ggtatgtcaa gtggtggaga atctgttgca agtggattta ttttcgtatt
      421 accagcagtt atattaattg gaagtcaaat tacattctta gaaggcttag ctgttggagt
      481 tggtggagtt ttatttggta taggaattgc tgctatagtt cacaactatc taatagttga
      541 agaacatggt aaacttatgt acccagaatc aatggctata tctgagacac ttgttgcttc
      601 agaggctggt ggagattcaa ttaagtacat gggaattgga tttgtaataa gtggatttat
      661 tactgtatta actggttcat tcttaaatgt agcaaataat gtaatgagtc ttgtaggaag
      721 caaattctat aaatggaaat ttgatattga agtaaatcct ctacttttag gaattggatt
      781 tatagtaggg cttgaagttt ctttaacaat gtttgcagga agtattttat caaactttgg
      841 tatagcacct ttaataggat actttacaga tatggctaaa gatggagcta tggtatggaa
      901 taatcctgca atgcctctta accaaatgga tgttggagca atatctagta gttatgtaaa
      961 atatataggt gctggtatga tgctttgtgg aggtattatt ggtgcaataa aacttatacc
     1021 aactataatc gcttctataa aagaaacact taaagctaaa tcaagcactg gagaaggaga
     1081 agaaggttca tctatacaaa tgatactttt gttaggtgga gtagttattg gatttttagc
     1141 agcattctta atttctggta acattgtcat ggcaattatt ggagctatca tatctctttt
     1201 attatcttta ttatttgtca tagtagctgg tcgtttaaca ggaacaatcg gaacatcaaa
     1261 tcttccagtt tctggtatga ctatagcttc tctagttatt gtaacattag tgtttgttat
     1321 aatgggatgg actgatttag aagctaataa atcattactt ttatttggtt catttatagt
     1381 tgttgctatt gctatagctg gaggttatac tcaatctcaa aaagtaactt atattattgg
     1441 tggtagcaaa aatgaaatgc aacgttactt tacaattgca agtatagttg gtgttatagt
     1501 tgttgtagga gtaattttat tactttctga ccaacttaga gcaactggtg ataatgtaca
     1561 atttgcatta ccacaagcaa acttaatgtc aacattaact tctggtatta tgtcaggtag
     1621 tttaccttgg gttatgatta ttgttggagt atttatggca atcgttctat atgcattaaa
     1681 tttaccaata atgactatag ctataggatt ctatttacca attgcaacaa cttctataat
     1741 attagttgga gcattaattc gtttatttgt tgaattggta tctaaaactg aaaaagaaaa
     1801 agaagttaaa gtttctaatg gtataagttt atcttctggt cttgttgctg gtggttctat
     1861 cattggttta ataggtataa tattacaagt aactggagtt gtaactccaa aggtaccaag
     1921 tggatttgct gcaacaaatt caatggcaat tgctttatta gtagttttag ttgtacttac
     1981 agcattacct attgttttat ctaaagtaaa aaataatgag caagagtaat gatgttttag
     2041 atattgtatt taaaattact gatgaacttg aatatgaagt agctgaaaat agtgttgtta
     2101 ctatacttga aaaacaggac cataaaattc aaaagttttt taggaagtta aaagctagaa
     2161 tacctaaata caaaaaaata gaactagatg aatattccag ttttgttttt ttacaaattg
     2221 atggtcaaag gactgttaga gaaataggca agaacctaga agctaaatat ggagaagaat
     2281 ctcatccact ttatgaaagg ttgttagtat ttttaaatca tattgaagtt aattgtcact
     2341 atattgaaag actggatata tagatataat aagaattatt atagatatat tagagcatat
     2401 aaattagaag gtaattataa aaattatgaa agaggctgtc tcaaaataga gataagcctc
     2461 ttttgtattg ttgagatgat taaaatatat ttgatttcac ataaaaaaga gtgtctcata
     2521 aaactaaaaa gttcattttg agacactccc tttaaaatat ttataaagta cagatttgta
     2581 aaacttatta aacttaagat tattttaata tcatttatta ttagctattt catcagctag
     2641 attatttaaa tattcccatc tttcatattt gtattcaagt tctttttcta gtttagattt
     2701 ctcatctaaa atttcttgaa gtttagtaaa atcagttgaa aattttgaag tactttcatc
     2761 aagtgttgca attttagatt cgagcttttc tatatcagag tcaatatttt caaactccct
     2821 ttgttcatta tatgagaact taagcttttt atcattttta ggtttttctt tttttggggt
     2881 agtagagtca tcttttttaa ctgattccct tttagattct tcaaattcaa ttccttgaat
     2941 ctctctataa ataagataat cactgtaatt acctgtatag atatgaattt tcccattacc
     3001 ttcatatgca aatattttat tacaaattct atctaagaaa tatctatcat gtgatactgt
     3061 aactactaca cctttaaatt cgtctaggta ttcttctaat actttaagag tttcaatatc
     3121 taaatcattt gtaggctcat ctaaaagaag cacattagga gctgtcataa ggattttaag
     3181 aagatgtagt cttcttcttt caccaccaga taatttacct atcaaagtat attgcatagt
     3241 accattaaaa aggaatcttt cacacatttg agaagctgta atcctagtac catctgaagt
     3301 ttctatatag tcactagctt cttttacata gtctatagcc ctcatatcaa tattcatatg
     3361 agagtcgtct tgagaaaaac aacctatttt aaccgtttct ccaattgaaa tatatccact
     3421 atcaggctct atttctccat taagtatgtt tataagggta gatttaccca taccattttt
     3481 gcctattata ccaattctat ctgtacgagt taaagtatat tctaaattat ctataacagt
     3541 attttcttca aatgatttag atatattgtg tatttctatt attttatttc caagtctgct
     3601 tgatgcaaca gatatatcga gattttcatc aggtttaaaa gagtctttat ttataagttc
     3661 atcaaatctt tgtaaacgag ctttttgttt agtgctacga gcctgagctc ctcgtcttac
     3721 ccacgcaagt tctgttctta ttaaattttg tcttttgtct tcaatacttg cttctagcga
     3781 aagtctttcc attttctttt ccaaaaatat tgaataattc ccatcataac tgaataatct
     3841 acccttatcc agttcaataa ttctatttgt aacacggtct aaaaagtatc tatcatgagt
     3901 tatcataagc aatgaacctt ttcttgagtt aagatattct tcaagccaat ctatagtatc
     3961 attgtctagg tggtttgtag gctcatctag tatcaaaaac tcacaaggtg taataagggc
     4021 tgatgccaga gagactctct ttctttgccc accagaaagt tctgctattt tttgattaaa
     4081 gtcatttata ccaagctttg taagtactga cttagcttca ctttctaaat cccaaagatt
     4141 taaggcatct attttttcgt gtactgataa taatttttta tttaattcct ctgtaaagtt
     4201 tgaattaagt ttttctagga tttcttgata ttcaccaagt agctttaatt cttcagaagt
     4261 tccattaaat acttgttcaa gtacagttga attttcattg taagttggat tttgaggtag
     4321 atattctatc ctaataccat tagtttttgt tatattacca gtctcacttt cttcaactcc
     4381 ggcaataatt tttagtaagg tagatttacc agtaccattt acaccaataa gtcctatttt
     4441 ttccccctca tttattccaa gagatatatt ttcaagtaat tttttttcag aatagctttt
     4501 acttatattt tccaatgtca ttaaattcat ataattcacc tttatttcta atctattatt
     4561 tttcaaaatg ttgataatct gggtttttcc aataaccacc ccaactccaa cctctgctta
     4621 caaaagcatt ataacaatca tcaccttttt ttatcattcc aaattttgtg ctagacctat
     4681 cagcataaac tgtacttact ttaggtgtaa catcatttcc agaaacttga ggattctgta
     4741 aagggtttat atctatagca cgtcctttac catgatttga aacgacattt gtaccagcta
     4801 tagttctata acaaaatgat gaggtattat tatcagacat agatttttca tctacagcat
     4861 catagtcatc tattaactta attttttcaa ttggatattt tttttcgtat aattccttaa
     4921 aaatatccac tacttctgga gctagttcac tgtttactat catttctcct tgatgagttt
     4981 tttcatcaaa tccatagtat gtaagtttta aataagatag actatcaaag cttataggtt
     5041 catcagtagg catagattta ccaatcattt tattttttat attatctggt attgaagaat
     5101 atgaaaaagt aggcttgata ttattatctt cctgcttttt ggtagagctt tgattatttt
     5161 tagaatcatc tgttgtatta tttttacttt gcatagtttc ttgactcttt atattatctt
     5221 ttttgttggg attagagaac agtatctcat atatagataa taaagcaaga aataatacag
     5281 ttattacaat cgttaatttc aatttattaa tttttcttcg ttttgacttt ttactcatat
     5341 tatcccccag tattagattc atataaacta tatatattat acctcataaa aataaactag
     5401 gctaacaata tttagttaac ctagaacatt tcatttatag tttctccaat tatttttatt
     5461 ccaatttcaa tgtcttcaaa agacaaccta gaaaatccaa gtcttaaagt atcaaaacca
     5521 gagttatctg taaaaaataa atctccaggc ataaatataa catttttttc ataacatttt
     5581 tctaataaaa ctctagtatc tatatttttt agttttataa atatatgcaa accaccatca
     5641 cccaagatgt attctgtttt tatatatttt tttacacatt tatatgcaaa gttaaatttg
     5701 tctccataga atttacgtat ttttttaatg tatttttcaa aacctccatt acttaagtaa
     5761 tcaaataaaa ttccttggtc taaaaaggat acatgtatgt ttctgcacct ttttacactt
     5821 tctagcttat tgattacttt tttatctgca aatatccatc caattctcat tccaggaaat
     5881 aatatttttg aaaaactacc aatatataag accccattat tcatattatc tagggaacag
     5941 ataggaaata tatgagaact attgtaaagt aattcttcat taaatccatc ttctataatt
     6001 gctatattgt attttttcat aagattatag aatttgtacc tattttcagg agtcattaca
     6061 attcctgtag gattgtgata agagggtgta atgtatgcaa actttatttt atttttatac
     6121 ttaactaagt tgtcttctag catattgaag tccaaaccat cttcatttat atctacacct
     6181 aagatattga ggttatgaga tttcataatt tttatagcag tattgtgagt aggattttca
     6241 catattatgt agtctttttc ttgggtaaaa gatgacataa taatatcaaa gccttctgtg
     6301 aatccatttg ttattagtat atctttatta cttatatcaa cacccttgtt tgtcatatat
     6361 tctagtaaat agtcaataag gggcttataa ccttgtgcat atccatagtt gagtaattta
     6421 tgtccttcta gtgaaattct atttaaaaag gattttttta attcttccat gtcgaataaa
     6481 tctccatctg gagaaatact tttaaaggaa attaggtcag agctccaagg aatttcactt
     6541 tttataatgt ccaattcatt ggctttttta gagtatgtat tctctaaaca atcccagttt
     6601 aacgaccatg tagtatttga agacttattc tttgatttaa caaatgtacc ttttcctgaa
     6661 atagagtaga ttaatccttc tgattttaat tcttcataag ttaaaactac agaatttcgg
     6721 ctaacttgta aaagctgact aagctccctt gtagaaggga gcttactatt gtctggaatt
     6781 agtccttttg atatcatgtc acttatataa tttttaagtt gtatataaat tggttcatta
     6841 tcatttaatt ttagatttga aaatatcaaa ttaccacctc aaattatttc atctaattta
     6901 aagagtattc tataagttta tctaatagtt cactgtactt aatacctcta gcagcagcac
     6961 ttctaggaat taaactagtt tgagtcattc caggaagagt atttagttca agaacatatg
     7021 gaataccttc acttataatc atatcaactc ttacatatgc tttgcaatta aaaattttcc
     7081 aacaagtctc agaaatttcg tttactcttt tttgaagttc ttcatctaag taaacaactt
     7141 cttcctttgc acctttttca actgagtatt tagcttcata atcaaagaat cctgaatcag
     7201 attttataga tattgtaggg aacacttctc catttaatat gaatgaagta tattctccac
     7261 ctggtatata tttttcaatc attacaaatt catcaacttc cagacctttt ctaactgctt
     7321 cttcaacttc atccttagaa tgaataaaga atgtggctac acttgaacct cctgagtttg
     7381 gttttataaa tactggataa cctatattat ctatttcatc atagtctatt tcatcaactg
     7441 attttactaa aacccatgga gcagtaggta gatttgaatc tctaagcatt tttttagtta
     7501 tatttttatc catacacatc ccactacaaa gaggtccaca tccagagtat ggtatatcca
     7561 tagtctcaag tattgattgt atacatccat cttcaccaaa ttttccatgt aaagcaagta
     7621 ttgcaaaatc tatatcttct ggtatctttg taaatatatc ttttttatca tctattatta
     7681 cctttacaac ttcgtattta tttttatcaa ggttgttata tatctctttt cctgaattta
     7741 aagaaacttc tctctctgat gatattccac ccattataac tgctattttc atattataca
     7801 ctcctatttt gatttttatt attttaaagt aaacttttta tttacatact taattatatg
     7861 aattaacagt tgaggatgaa agtaccacta tgtacgaagt ttatattatg tgtggtttta
     7921 gagtaataat ctagtttaaa gatatgttta aatgattttt taactttatt tatgttatta
     7981 tttttgctaa aattattgtt atgaacttaa gactagtctt aatggctata aatcaaggag
     8041 tgataaattt tgaaagctga aataataagt gtaggaacag aaatattatt gggagatata
     8101 gtaaatacaa attctcaatt tttagctaaa gaacttgcat cattgggaat agaagtatac
     8161 catcagagca ctgttggaga taacaaacaa aggcttttag aatgttttga tgaaagctta
     8221 aagagaagtg attttgtaat tactacaggt ggtcttgggc caactggtga tgatatgaca
     8281 aaagaaactg ctgcagaata ctttggtcag aaattagaac tacataaacc ttcactagag
     8341 gttttggaat ctttctttgt taaaacagga aaaaaaatgg ccgaaaataa tatgaaacaa
     8401 gtgtactttc caaaagatgc tatagtacta aaaaataata atggtacagc tccaggggca
     8461 attttaaaaa aaaatggtaa atttataata gtattgcctg gacctccaag agaaatgaaa
     8521 gctatgttta atgagagtgt caaaccttac ttacaacaat tcacaaatga aatgctagta
     8581 tctaagactt taagattata tggaataggt gagtcaaatc tagaattaga aattctcgat
     8641 ataattaatg agcagactaa tccaacagtt gctttatatg caaaagaatt agaagttaca
     8701 ataagaataa ctgcaaaggc tgaaaatgaa agagaagctt ttaaattaat aaaaccagta
     8761 gaagaaaaaa taaaaattag agttggcaaa tatgtatata ctgaaggaga tatttccata
     8821 tctgaagggg agactgcttt agaagatgct gtttctaagc tacttgtaga aaaaaatcta
     8881 acaatagcag tggctgaatc ttgtactgga ggtttggttt cttcatctct tataaactat
     8941 ccaggtatat cttctgtatt tcttgaaggt tgtgtaacat attctaatga ttctaaaatg
     9001 aaaagattag gagtaaaaag agaaactttg gaagagtttg gagcagtaag tgagcaaaca
     9061 gctatagaaa tggctgaggg cgttgctaaa ggacttaaag ctaatatagg tatatctaca
     9121 actggagtag ctggacctgg tggaggaacc aaagagaagc ctgtgggact agtttatact
     9181 gcaatatata taaatggaaa aacaatagta aagaaaaata tatttaatgg ggatagaaga
     9241 aagataagat taagagctac tagagatttg cttaatgaat tgagaataca attagaaaag
     9301 ctttaagttg ttatgatatt ggatatattt gaagtgttac tataatataa aattgttttt
     9361 ggaggctttt aatttatgat aaaacatata ttttgtgatt tagatggaac tctatatgaa
     9421 aatggaacca taacaaaaga agatataata gcaattgaag aaatagaaaa aaagggagta
     9481 cagtttaatg ttgctacagg tagaattttt aaacaagcac gtaatattat caaagatagt
     9541 ttagatatga atggatatta tgtatgtgaa aatggttcat ttatatatga taaagattat
     9601 aatgtgattt ttaaaagaac gatagatgat aatttagtaa aaaaagtcat tgatagattt
     9661 gagtcaagtg atgcacaact ttattttaaa tataagggtg atgtaattgt gggtagtgat
     9721 accactgctt ttagacatta ttctagtgac tttatagttg acccagattt tgaaaaaaga
     9781 agtagttttg ataatcttat aggaaatata ggcgtatgtt ctgaaaattt ggaagagtta
     9841 tccagaatag aactttatct aaaaagtgag tttagtgaag tattagaaat atatttttct
     9901 ggaacatata ctttaaatat agttcctaaa agtgtgtcaa aaagaggaag tatagagcat
     9961 gtaataaaaa cgttaaatgt aagtccagat gaagttgcta ctattgggga ttctccaaat
    10021 gatatttgca tgttagaagg atttaaatat agttttgcaa tgtctaaggc aaggggagat
    10081 gtaaagcaaa gtgcaaatta tgtagtagat agtgtaaaaa gtgccataga tgttataatg
    10141 gagataaata gttagcataa aaaatgactg tcctagagaa gtataaaatt atattctata
    10201 gagcagtcat tttaattatg ttcaattata agtttaaaaa ttcttaagtt aaccacaatt
    10261 tgctatatta tatgatttta ttgtgttaga taaaatagag tcaagtatat aatttatgac
    10321 actattacaa gtataagctg aaaattttaa atcattcata taatcagttc tctttttgta
    10381 atcactataa agtttagtaa aaaacttctc aaagctacta tttatagtac cacatttaat
    10441 tttagataaa tcaacatctt ttgcaaatgc tccaagttct ctttcataag taacatgttt
    10501 atttaagcta tgcttaaatt cccaacgttc actgtgagct acacagaaaa aatcacataa
    10561 aaaatgacat atgactccta gttcctgact aagtctactt actgaaaagt atttagaaag
    10621 actatctata ttaagactgc atagatagtt tattttagaa acaatcatat taaatgactc
    10681 atctaaataa tgttttttta atttatattt agaaaaagca tcaggcttta tattgccata
    10741 tataaaattt ttttcgctta taaagaatgt tttattttca tctatattat caagtattga
    10801 ttttgccata ataaaatggg tattcattaa catatttaca cctcgtatag tttattagac
    10861 ttattaaata tattattatg atatgatatt tatgataaaa gtttaatggt aaatatatga
    10921 aactaagtca atagtattta tttaaataat ttcaaccata ttcataatac cagtaaatgg
    10981 ccaaatttac aatatgtaaa tagtgttaag tttttattta tcaaatttaa aattaagtat
    11041 tagtatactt tctaattaac ctgttggctt tagactgtga tatttcaagg tgtttggcta
    11101 ctttataaga actaccaaat ttttcataag catctaaaat tatatttttt tcgtactctt
    11161 taattaaagt gtcaaaattt ttatttttat aattagtaga ttttgagtca gatataataa
    11221 ccttattaat ttgggatata tgtgttttat ttccattatt ggtaatatgt atattttttg
    11281 taagaatgtt tggtaagtca tctattgtta tatgattatc tgtacaagta agcaagattt
    11341 tttgaattat attttgtaat tctcttatat tgcctgggaa tccataattt gcaatagcat
    11401 ccatagcttc tttagaaata gttttattta ggttgaagtc acttgagtat ctattaaaat
    11461 aatattttat aatttccact agattatccc ttctttctct aagaggaggc aattttattt
    11521 ctataacatt taatctatag tataaatctt ctctaaactt tttttctttt acttttgaaa
    11581 ctaaatcagc atttgtagca gatataatcc ttatatcaac ctttttattt tttaagctcc
    11641 caactggagt aaaagttcta tcctgtatta attgcaaaaa ttttgcctgt agattttgtg
    11701 gtatatctcc tatttcatct aaaaaaagag ttcctccatt agctaattct acgagcccaa
    11761 ctttaccttt agtacttgca cctgtaaaag cacctgaagt atatccaaaa agttcagatt
    11821 caagaagttg tggagatata gttgcacaat ttattgttat aaatgggcca ttagatctat
    11881 tgctgttgtg gtgtatatat ctagcaagtg agctttttcc tgttccagat tcacccaata
    11941 aaagtacact tacatccgtt ttagaaacat gatttactgt gtttatgatt ggtctcatca
    12001 ctattccgtc cataattaca gtatcttcaa ttctaagtgc agttctgtat agtgtttcta
    12061 cctcagattt atattttttt acttgctttt ttgtgtcttc aagtttattt tttatattat
    12121 taagttcagt tatatctcta caattttcaa cagtaaattc taagtttcca ttttcatcaa
    12181 atataggggt agcagttatg ataagctttt tccctgcttg agtatcttgt tccagtgaaa
    12241 atttagtctt tgtttttaat acaattggta tggggctttt tgttgaatat ccagagtctt
    12301 ctaaaaaaga aacatttttt cctaaaatat cttctttttt tagaccataa ttttcttcaa
    12361 atgctttatt acaatatatt attattcctt tatcatcaga aacacatatt tcatcatggg
    12421 aagcttctaa tattttttga taaaattcca aatctaattc caaatttaaa ttcattttta
    12481 tattttcctc ctaaattttt aacgatatat taaaataggt ttaattgatt aatatactta
    12541 aaatttgata tttatataag tttcagtcta ttttaacttt aaataagtta tatataactc
    12601 attttacaat taaaataagt ctaaatcaac ttaaagtaag tgaaaaataa atataaaaaa
    12661 attgaaaatt tattagtatc gttggaaata ctagggtttt agagtcgata tatattggtt
    12721 taagatggca tagtttttgc ttaaaaagaa gagtgtaaat tgactaaata aaaatagaag
    12781 ggaatttaaa atgggagaaa aaagtaaagg gtatgtattt atagcaatag cagggttatt
    12841 atgggctaca cttggattat ttggaaagtt tttaatggga aatggattga catcagaaca
    12901 agtagcattt acaagattgt tttttggatt tatagtttta ggcgtatatt catctataag
    12961 aacaccacaa atcttaaaga taagtaaaaa aggaattata tattcagtaa taataggtat
    13021 tatatgtcag gcaatgttta atttatgtta ttttaaagca attgacattg cgggtgtatc
    13081 aattgcagca gttttactat atacatcacc actattttta gcaatatttt caaaagtatg
    13141 ttataaagaa aatatcacta gaagcaaatt attttcactt attctttgtt ttattggtgc
    13201 aattatggca gtgagtggtg gacgtttgga ttttcaaggt ttaaacgctt ttggactttt
    13261 tcttgggaca ttatcagcaa tagcatatgc acttatgcca actattagta aaaatgcttt
    13321 aaaggaattt tcaagttcaa caatattagt atacagtttc ttatttggag ctatatttat
    13381 gattccttct tcaagacctt gggaaatctt aaattatgct aaggatttag atgttttatc
    13441 ttgtatgcta atgttaggca ttgttccagc tgcacttgca tatatttttt atgcaacagg
    13501 tatatctaaa ggtgtagagt tgtctgttgc aggtgttgtg gcatcagtag agttagttgg
    13561 ttcagtaata attggttgta ctatattagg agaaagtttt tccttaggga agttatttgg
    13621 agtgatgttg atgttaatat ctgcagtagt agcacttaat ctttcttatg atgaaataag
    13681 aatattttat aagtccaaca agttaaagca gattgaaaaa actgaaagta tttagaatta
    13741 tatatttaaa aaggatttat tggtatacat tgtagagtcc aacaaaaatt ataccaaatt
    13801 atatttggtg ttagaaaata actatttata aattttgtag aataaagtgg acagtacaaa
    13861 taaaatataa gtagatacag tttagattat atatttttta ttatatattg tataaatata
    13921 caaattaata ttttgttttg taaacgctaa ataactatag aaaggattat atttatataa
    13981 ccctttctat agttattatt ttgtattaaa caaacttaca gctctgataa gaatttctag
    14041 cctttaaagt atcatcaatt tgatttaaaa tttcccattt ttttaggctc catataggac
    14101 ctactagttc atccctctta ccatcaccag tgagtctatg gattatcata gtttcaggaa
    14161 gtatttctaa ttggtcacag acaagattga tatattcatc ttgttccata agagttaaca
    14221 taccattttg taacatcttt tccataggag tatcttttat aacatgcaat agatggattt
    14281 ttataccgtc tacacccatt ttaccaactg cctttgctgt ttccatcatc atattataat
    14341 cttcaccagg aagaccattt ataatgtgta cgacaacctt tatattttta gactttaatt
    14401 tttcaacacc ttcaacaaat gttttataat catgacctct atttataatt ttagatgtag
    14461 aatcatgaat tgtttgaagt ccaagttcaa cccataaatt ggttctttta tttagttcag
    14521 ataagtattc tacaacatca tctggtaaac agtcaggtct tgtggatatt gaaagaccta
    14581 tgacatcttc aagttctaat atagtctcat atttttcttt taaaacttca agaggagcat
    14641 acgtatttgt gtaggcttga aagtagccaa tatactttgc atgaggccat ttttttaaca
    14701 tcatttcttt tatattataa aattgactga taagattatc tttagggttt ccagcaaaat
    14761 cacctgagcc ttctttgcta cagtaggtac atccgccata accaagactt ccatcaatgt
    14821 ttgggcaggt aaatcctgca tttatagata ccttaaaaac tttttctcca aaagtatttc
    14881 taagatagta attccaagta tgatatcttt tattattaaa agcatattta aatttatcca
    14941 aaaaaatcac ctatttctaa taaaattgta cgctgagctt gtacaaaaaa catattacca
    15001 caaatagata acttaaacaa attctatttt aagtaatata aaaatacagc gagaaaataa
    15061 ttaatatttt ctcgctgtat ttccagagaa aaatagaact agtccaaata atattatgaa
    15121 aaaagacatt atcgatacta taaatctaga taaaaaaagt ggagataata taaggcatat
    15181 accaaataga atcttaacta tattccaagt tgtaaattta gaataaggat tattcttact
    15241 atttacaagt gctactagtt gattaaagat taaataaaat cctaaaaata atgaaaatat
    15301 gctttgtatt ttttgaggaa atacaaaaag taatatacat gcaattatta gaacccattg
    15361 gatattaata gagggtttaa tttcttcttt agtagcatat ctttttaatt cattaatatt
    15421 ttttaaattt aaatatgcaa agaataaaag tgcaatacca aaagtccaag aaattaattt
    15481 tatacctaat gtcttaaatg taaggcataa taatcctaat ataatgagta atactcccat
    15541 taacataaat ttagtagcat tttctttttt attaaagtta ttaaaatcga tattaaaatt
    15601 tgtgtacata aaatcacctc caaaatcgat agaacaatta taacagaaat tcatatccaa
    15661 ataaagccta gtgtataaaa attaaactat acttagtaat taattaatag gttgtatcat
    15721 aaacttctat taatagaatt atatagaagg aggtttgtcc atgacaaaat tatgggcaat
    15781 aatatttgca ttattagcag gtggttctac agctcttgaa gcttttataa atggagaact
    15841 aggaaaaagt acaacagcat tagtggccac ctttataagt ttaactgtag gagcaatatt
    15901 ttttttggca agtataatag ttacaggtga cttaaagtgt ctagtatcta taaatggttt
    15961 taattataag ttgttattag gaggagtatt tggaggtcta ataatatatt ttacagtaaa
    16021 ggcaattcca aatttaggag tatcaaatac acttatatta acacttgtat cccaagtttt
    16081 agttggattt tttattgata gcgtaatttt gggacaagaa acaatgcatt tatacaaata
    16141 cataggggtt atacttttaa tattagggac attctttata gtgaattaac aatttaatat
    16201 aatttgctat gcaactcaaa aatttggtat aattatgtta ataaaacaaa gttagatgaa
    16261 agaggtgtta aaattggctg atgaaaaatt tgtaatgtca tttagtggtg gtaaggatag
    16321 tatattagca ttaaatcgaa tgttgaaaaa aggatataag cctgtggcac tcttgacaac
    16381 tataagtgaa gaacatggaa agtcatggac acataattta gaatataata tgcttaagca
    16441 agtaagtagc aatataggat tacctttatt agttgctgag tgtggtgtag aaggatatga
    16501 agaaagcttt gagagggctt taataaaagc taaaaacatg ggagcaacaa tatgcgcata
    16561 tggagatata gatatagaaa gtcatagaaa atgggatact gatagatgtg aagcagttgg
    16621 tatgaaagta gatttaccat tatggcaaga gagtagagag gatttagtat atgaatttat
    16681 agacagtgga ttttgtagtg ttgtaacaaa agtaaactta aaacacttag gagaggaatt
    16741 tttaggaaaa aaattaacta gagaacttgt ggagaagata aaaaatgctg gtgcagaccc
    16801 atgtggagaa catggagagt atcatacatt tgtcgtagat ggtccaatat ttaaaaaacc
    16861 tgtagaatat gaagtaaaag gaactttaat aaaagatggc tatggatatt tagaaataaa
    16921 ataattacat aaataaaata cctattatat aatcctaatt tttgcaaagg tttaatattt
    16981 gcaagattgt ataataggta ttttttattt agatattttc atatattgcc actcttatca
    17041 tctaagcaac cttattcaga ttagagtcta atgtagaaaa attctttttt agaaaaacca
    17101 atgataaaat aagacatata aactcagaaa caatagtagt catccatatt ccttgtccac
    17161 caaatattag tatcattgaa attagactta aaacaactat tacaagcccc ctaccaagag
    17221 atataacagt agaaattgat ggtttttcta ctgatacaaa gaatccagaa attattacat
    17281 taattcctac caataagaaa gatatagaga atattttaaa tacacgaact ccttcattga
    17341 atagcattgt ttcttctggg tgtataaata aagagactat tggttctgaa aaagccatac
    17401 aaattgcaaa tacagcaatt gatgctatta ttgtggcttt tatccccatt tttaataatt
    17461 tttttacatt atctatattt cctgcaccat aataaaagct agtaagtggt tgcatacctt
    17521 gagttatacc catcatagtc ataagtacta atgtatttat ataatttatg acactataat
    17581 aaataagagc attttctcca atatatttag taagacttaa gttaaaaagt aaaacaacta
    17641 taccacaact taattcagta gttgaatcag gaaaacctat aaatacaatt tttctaagtg
    17701 tcttaaaatc aattctaaat tttgaaaaat tcaaagtaga gttttttctt aaaaaatgta
    17761 ttaagaagaa aatagtagaa aagacttgag acaaaccagt tgcaatacca gcacctttta
    17821 caccccatcc aaattcaatt acaaacaagt aatcaagtat gatgtttgta agagcagata
    17881 ttattacacc aacagtagct aatattggaa agccatcagt ttttattata acttctaaag
    17941 aataagacac tataaagaat ccgttaaaaa atattattat tcctaaataa tctttaacca
    18001 taccaattgt agattctgta gctcctaaaa atagtgctaa tctatctaaa ttaaaaaaag
    18061 ttatagctaa tataattatt gataatataa ttattgatac taaattaaat gaaaagactt
    18121 cgttagcaga tttaatatct tttttcccca gataaattgc aattatagta gatgcaccag
    18181 ttgaaaataa tagagaaacc gcaaatataa aattaataaa aggcattgat atattgacag
    18241 cagctaaagc taattctcca actcctttac ttacaaatat tccatcaacc attgtatata
    18301 gtgagaaaac ccacattgca actacagatg gtgctaggta tttaaaaaat cttttaccta
    18361 gactcatgtt ttcgtttata gatatttttg tatccattta ttatcactcc ctgtaaatct
    18421 attataaaat tacgaaacta ttaattaata attttatttc taatataaat gataaactat
    18481 ggtataatac caatgtcaac agcaaatagt atataattat aaattttatt aaataatagt
    18541 gattttaagg gtcttataaa tatatcttat ttatatatta ctgacattta actgataaat
    18601 aggaaaaagg tactttaagt gacatttaat tgacataaaa ctgaacatta tttgaatagt
    18661 attttgctta tattttcaga tgtttcttta tccatttcat ctaataagtg agaatatcga
    18721 atatctattc atagctattt ttatatctgc atgtactaat attttataaa tagcttttat
    18781 gttagtacct gaaagtaata tcaaagttgc atttgcatgt attaattcat gtacttttat
    18841 atctatagga ttattatatc ttaatagttt tttaaaatga acaacaaaag ttggttgaac
    18901 tcacgggttg aatcttttat ttaagcatat caaatcaaat tcatttttca atatagcctg
    18961 taattttaac ttattttgtt taaatttttc ctcttcaagt aaacttataa gttcatcagg
    19021 agcaaataat tttcttttgg atttaggggt tttagggtct ttgaagatta ttttattgtc
    19081 tacataaact aatgtttgat ttacatgtat gattttttta tcaaggtcta catctgacca
    19141 tataagtgat acagcttcac caaaacgtaa tccaagagtt aacatcaaaa aaattggtat
    19201 acctatagga atattttttg atttttctaa taataattga gctttctgtt ttgtataaaa
    19261 cttagaagtt gaactatttt ttttgattag ggttgtaaca aaatcacaaa tattttctga
    19321 tatttctttt aatctataag attctttcaa tactgatttt ataaaaccga cttggttttt
    19381 tgctgaattt aacatgtgct cattgtatat gttatttata taagactgta aaatagaagg
    19441 cgttatatct attaatttca tatgttattg gaaaatcgaa taataataac ttggtattcg
    19501 aaacttttga taaagtaata gaactatatc caaatgcaaa acctgttttt catagtgata
    19561 gaggttttca gtatactagt aaggtgtttg aatcgaaact tttagcacaa ggtatgatac
    19621 aaagtatgtc aagagctggt cgttgtattg acaatgctcc tatggaagga atttggggaa
    19681 ttttaaaggt ggaaatgtac tatttaagga aatttgatac atatgaacaa ttagttaaaa
    19741 taatagagaa ttatatatat tactataata attttagata tcaaaagaga ttaaattcta
    19801 tgtctccact tgaatttagg aaatatttaa gtacagagat agcataattt attttaagta
    19861 gctctaaaaa aaattaaata tctagggttt tatttgacgt gctctttttt agatagtaaa
    19921 ctttaaaata tagatattaa ttatatgaat ataataaaaa aagtactaat gagttacact
    19981 agtactttat aactactttt acatgtttta actgtataaa acagtgggta tagttcaggc
    20041 aggagtgact ttagttttga actaaaaatc aagttcaaaa agaataaaca ttagtatttg
    20101 aacttcactt tacatctaaa tagattgtag ttcttcttgt ttttattata ccacaaattg
    20161 gtacagatat tcaaaaataa tatatttatg atataataaa aatgtaaata gttttgcagt
    20221 gagcgatatt tgttacaaag tagggcttaa cgcttgaaat ataaggtgtt gagggtatat
    20281 gataaacttt atcatttgca ctactcatgg ttcactgcaa atttaagaga gttgcacatg
    20341 tgtaagtatt gaaaatgccc agtttatttt ggggttttat attaactaag tggtatgtaa
    20401 ataaagttaa atttattaca tctaattctg tctcttcatg ttttatatta actaagtggt
    20461 atgtaaatac aacagcacca taaaaaaaac cattcctaaa aaactgtttt atattaacta
    20521 agtggtatgt aaatagtata atgttgaaaa gttagagagt acaatcaaga agttttacat
    20581 taactaaaat gtatagaata aaacatgtat ttatacttaa attctgtacc tatataaaaa
    20641 agtgaactct gtcaacaaag cactttttta tatagatgaa ttatcatttt gttttaagat
    20701 agaacatact aatgataaat gtttatcatt agtatctgta tgtacataaa agtttaattt
    20761 tttatataaa tttgctcttt agaaaaatgg gcagtatcaa taaatatatt gtctaaattt
    20821 tttttaggaa ctagttgact agccataaga ttagcttcaa ctctttgatt gttagactat
    20881 gaaattaaat ttaaaggttc attcttagtc atataaatag ctttattatt cgtatgtact
    20941 ataacaattt ttgccatctg cttttgatag ataaagagct ttatcagctt tagaaaataa
    21001 atctttatat aatttagttg aatcatcagt gaaggcaata ccaatgctta atgttatttt
    21061 atgattgtcc tttactttta ttttacttgc atcatttaaa atatcattag taatttttat
    21121 tattaaatcc tttgttaatg attttataaa tattgtaaat tcatctccac caatgcgagc
    21181 aactatgtct ttatcatgtg tgttatgtaa tagtattttt gatacagcaa ctaaaacttc
    21241 atttccctct aaatgtccaa aagtatcatt tacagtttta aaatcatcaa tatcaataac
    21301 taatagagca tcattatttt tggaatcaga actacataaa tactcttgaa taagattttg
    21361 tgatactctt tgattataca attgtgttag tgggtcacgt tgagcctgat taattaagct
    21421 ttctaattta gatttatctt cagttatgtc ttttaaaacg cctattgttt tgatggggtt
    21481 atggtcacta tcatatataa ctgaacattg aacacgaatc catctccatt catttttagt
    21541 taatgaattt atttgtctta tctcaattgt ttcacgaaga tttccatgaa gtacttctag
    21601 tagttttcca atatcatcaa ggtgaataat tttaccaaat tctaatagtt tggagtaatt
    21661 ttttgtttca aaattttcta attcaatttt tttatcaatt tctacacgtt gatattttat
    21721 aagggtatcg ttttcaatat catactcaaa catgatatct gtaatatttt gcaaagcaat
    21781 acgatatcgc tctctttcta attttagttc agattctatg ttttttagtt gggtgatgtc
    21841 agtaataata ctattaattt ttactatgcc atcactattt aacgatttca ttccggaatc
    21901 taatacccac attaatgttc catctttttt tcggattcta tattcagatg aatatgtagg
    21961 tccttttgca aaacatcgtt cacaatcttc tagtgcttta ggtaagtctg gtggatatac
    22021 agcacccact gctgaaccac cactcatttc catgaactca tcatatgtgt atcctaatat
    22081 tttgggcaat ccttcattga catagaaaaa tgagtatgta ctatcatcat tgctaccttt
    22141 taagccacca ttgatattat ttgtaatcat ttcaataact tctgtttttt cctttagtaa
    22201 tctttgcaaa taatcataac tttgagttcc aattttagtg ggaaagtatt catcaagttg
    22261 catttcacca taagggtttg atatgtgcat gtgctttact aataattttc ctctatcctc
    22321 aattatctca aatgtacaac gttgatttac ctctaaaatc atttgagcat tttctttagt
    22381 tcgaacagta tattttccta ataccataca tctattttca tcttcactta cagtctcaaa
    22441 tatatcatta tttatatcac aagaaggaat ttcatcttga cctgcataaa aataattttt
    22501 tatttcatta aaagaagtga gaaattcctg ttctcctggt ccaatccatg taacattttc
    22561 acaaaggtat tttaatactt catcaatatc tcgatttata taaaaacttg ttataatatg
    22621 ttttgctaat ttgggtgctt tttttaggag tatgctcatg atattcctcc tttctttttg
    22681 ctattatatt ttaattatat tatacatcaa tatatttaaa ttaaaagctt tgttaaacta
    22741 tgatatcttt gttaaataag ttaattttaa aaaaagaact gaaacaatat atgtttcagt
    22801 a
//
LOCUS       DAEPXF010000052        22572 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain HB27
            SAMN13376389-rid12479423.denovo.056, whole genome shotgun sequence.
ACCESSION   DAEPXF010000052 DAEPXF010000000
VERSION     DAEPXF010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13376389
            Sequence Read Archive: SRR10518432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 22572)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22572)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001069624.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001069624.1
            Genome Coverage       :: 315x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/15/2021 01:54:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,165
            CDSs (total)                      :: 4,076
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 89
            rRNAs                             :: 10, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 10 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 19 of 69
            Pseudo Genes (incomplete)         :: 44 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 10 of 69
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22572
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13376389-rid12479423.denovo.056"
                     /strain="HB27"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="China: Hebei"
                     /collection_date="2017"
     gene            102..843
                     /locus_tag="KRL67_001717"
                     /pseudo
     CDS             102..843
                     /locus_tag="KRL67_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428529.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
     gene            1104..1358
                     /locus_tag="KRL67_001718"
                     /pseudo
     CDS             1104..1358
                     /locus_tag="KRL67_001718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729170.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1368..1547
                     /locus_tag="KRL67_001719"
     CDS             1368..1547
                     /locus_tag="KRL67_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH1522600.1"
                     /translation="MIHVEISEKEFFRKIEEAFNKGEGNVEIDNLGDIKIQVKKFDDK
                     TKSLELKLIKEFKLK"
     gene            1559..1912
                     /locus_tag="KRL67_001720"
     CDS             1559..1912
                     /locus_tag="KRL67_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3139 domain-containing protein"
                     /protein_id="HBH1522601.1"
                     /translation="MRKYKAILLTLIISIVAIIGFIIYNNYFIKWNEVEKYIDKYMTY
                     QVVLDDDIERITKETYTKENYDGILYKSFYKNYPDYEYQYFYSGDYYALHVNKVMLQA
                     YHEEDGSKLQKIIKN"
     gene            1987..4542
                     /locus_tag="KRL67_001721"
     CDS             1987..4542
                     /locus_tag="KRL67_001721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HBH1522602.1"
                     /translation="MGIYLKMSLAYLKKNKLRTLLLIIGVMLGVMLIFGPSVIKDSQN
                     ENNVVAIHELYSGYHVEFSDLTLDDAKKLETDGKVSKGNSVQNLGRISDKKGNSFILT
                     SSNEEYLKSRYNKIIEGRLPKNNNEIVLEKKALEAMNLSEGVGSTLNLTIKKNYKDTN
                     GSNQLFSINKEFKIVGVMEKPKGYYDVVWQYEAVTYGNDETNNIIPHDAISYNSILSF
                     KSGWRNIEGECDYLMKKYKLGQKSFLPNVPLVRELRYVEDDKNDPNVFKRNVLITTVS
                     IIFIFNIFNITLNQSVKEMGLLRLMGAKKRNVRCMIIYQALIITIIGILLGLVAGVTY
                     SYIGISNYNEVLYSEAGLNPKLYINNEIVIKATLVGIISVFISCIIPTFKIGKISPIE
                     SIINTDKIKNHRSRLGIDKLASKIFGFYGFMGLKNIGRNKVRAAISMLSIALGGYIFI
                     TTFSSMQEEVNSKIEDMQNRYNITMQFGVNADPQISKYTDSDVENIEKIKGVKSIHKI
                     QITNGMFTFNNKDINKEFTTYNGVEKKEIMEDKLVLKLYDDDYIDEKLNDFVEEGSLD
                     DIKKDTDGYPNVAVYNYFYDIVDDHTIKPIFKELNIGDIITVKIPVEENNTIVYKEMK
                     VRVAVILKDEWIAMGDGDFMPDLEIITSNIHSKNITGEQKYTQLGINLEDPYDEIVNN
                     EIQKISRSIPGSLFNSRLSYHEMSESIYKSYIKSQIAIIALVLIIASINIFCTIRTNL
                     LIRKKEISTLRALGVSLKNMKKMLIYEALAYAVFSFIIAMIPSFINLVKFTNWNNDAY
                     INYGIENFMSFTFPIKESLLFFAISVFVCLIAVVVSNRDFESMNIIEGIKENE"
     gene            4559..5221
                     /locus_tag="KRL67_001722"
     CDS             4559..5221
                     /locus_tag="KRL67_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBH1522603.1"
                     /translation="MKLLKVIDIKKIYGKGENEIKAVDGITLEIEPHKFSAIIGQSGS
                     GKSTLLHCMAGLDKPTLGDVFMDNLDLYTLSDDKLSNLRSKEFGFIFQNYNLIPVINV
                     YDNIVLPILIAKGKIDNNYINDLITKLGLESQINKFPNELSGGQQQRVAIARSLANKP
                     SIIFADEPTGNLDSKTTDEVMNLLKYCVNEYKQTLVMITHNDEIAKMADCIITISDGK
                     VI"
     gene            complement(5510..5620)
                     /locus_tag="KRL67_001723"
     CDS             complement(5510..5620)
                     /locus_tag="KRL67_001723"
                     /inference="COORDINATES: protein motif:HMM:NF025126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH1522604.1"
                     /translation="MKHSINFKRFLSRGNQNVLSEFILVVLAHNISKFHK"
     gene            complement(6233..6805)
                     /locus_tag="KRL67_001724"
     CDS             complement(6233..6805)
                     /locus_tag="KRL67_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class B sortase"
                     /protein_id="HBH1522605.1"
                     /translation="MKILVKILINIICIITLIFSSLSAYIKLSEYRKADEVYTELREN
                     TSNNNSKHQELYDKNNDYRFWLKINNTNIDYPVVQGYNNDFYLTHNFYKNYLPSGNIF
                     MDYRNNFENDKSLIVYGHYMKNKTMFGQLENYTDEALFKENNLVETKYKVQTYTYKIF
                     SIYTADLIYRDYLSIHFNNDEFKDSLNYIT"
     gene            6975..7112
                     /gene="rpmH"
                     /locus_tag="KRL67_001725"
     CDS             6975..7112
                     /gene="rpmH"
                     /locus_tag="KRL67_001725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HBH1522606.1"
                     /translation="MSKRTYQPKKRQRSKEHGFRKRMKTSNGRNVLKRRRAKGRNRLT
                     H"
     gene            7190..7534
                     /gene="rnpA"
                     /locus_tag="KRL67_001726"
     CDS             7190..7534
                     /gene="rnpA"
                     /locus_tag="KRL67_001726"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HBH1522607.1"
                     /translation="MDFNRTKGLKKDSDFRKVYKHGKSFANKYLVIYILKNKSDYSRV
                     GISVSKKVGKAITRNRVRRLIKEAYRLNIDEKIKPGYDIVFIARVSSKDATFKDIDKS
                     IKNLVKRTDISI"
     gene            7566..7817
                     /gene="yidD"
                     /locus_tag="KRL67_001727"
     CDS             7566..7817
                     /gene="yidD"
                     /locus_tag="KRL67_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HBH1522608.1"
                     /translation="MNKIIYILKEISKYLSNLCIYLVRFYQKYISPLKGPTCRFYPTC
                     SQYSIEAFKKYGFVKGMYLTIRRLLKCHPFHPGGYDPLK"
     gene            7849..8556
                     /locus_tag="KRL67_001728"
     CDS             7849..8556
                     /locus_tag="KRL67_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HBH1522609.1"
                     /translation="MNIIGNFLGLILKVIFEFVNHYGISIILFTILVKIILLPLTIKQ
                     TKSTKAMQDIQPRIKEIQEKYKNKPEKQNEEIVKLYGEAKINPLSGCLPLLIQFPILI
                     GLFSVLREPVAHGVFANKAAFLAADNGFLWIKSLTSPDYVLAVFSGASAYVMQKVMTP
                     KDQLQGSMKVMTYVMAGMSFYWGFIFPAGLTLYWTVSNLFSIAQYYLIMNPLKAKLAA
                     NSKEEKVNEKKPKINKK"
     gene            8525..9154
                     /locus_tag="KRL67_001729"
     CDS             8525..9154
                     /locus_tag="KRL67_001729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein jag"
                     /protein_id="HBH1522610.1"
                     /translation="MRKSLKLIKSKSKEEAISKAIAELNLKEEDIEVEILENPSKGFL
                     GFIGAKDGTYEIFVIEREVDVAKNFIEVMLKNANVDAKVNVSQKDNLIMVDIEGKEAA
                     SLIGRRGETLDSIQFLTGLALNKINKDSHTRVLVDIENYRSKREESLIRYANKVAREV
                     AKTRKTKKLDYMNPYERRIIHSALQNDKYVITYSEGTDPYRRLVIECKR"
     gene            9266..10645
                     /gene="mnmE"
                     /locus_tag="KRL67_001730"
     CDS             9266..10645
                     /gene="mnmE"
                     /locus_tag="KRL67_001730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HBH1522611.1"
                     /translation="MFIDDTIAAIATAPGEGGIGILRISGERALKVAEEIFKSMSGKS
                     IEEYNKRTLIYGNIVDNENIIDEVLLAYMKGPNSYTGEDVIEINCHGGFISVKKILEL
                     ILSKDVRLAEAGEFTKRAFLNGRIDLSQAEAVIDVIKAKTDIAHEVAQNQLEGSLSKK
                     IRELRDKVTEILAHVEVAIDYPEEDIEHITYQTLKEKTDELKKEIKKLYDTAESGKIL
                     REGLKTVIVGKPNVGKSSLLNSILGENRAIVTDIPGTTRDVIEEFVNIKGIPLKIVDT
                     AGIRDTDDIVEKIGVEKSKESFTSADLIVMVLDASRKLSEEDIEILEKLKDKQTIVLL
                     NKNDLKQEIEEEKILKYVENNSIIKISALQQEGIEELQDKIESMVYKGSIKNNSSLVV
                     TNSRHKDALSKAYKSATDALIALEQSMPFDFVEVDLKNIWDYLGYINGDTVTEDLLDN
                     IFHNFCIGK"
     gene            10740..12635
                     /gene="mnmG"
                     /locus_tag="KRL67_001731"
     CDS             10740..12635
                     /gene="mnmG"
                     /locus_tag="KRL67_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HBH1522612.1"
                     /translation="MIKFEAGKYDVIVVGAGHAGCEAALATARMGYKTLIITMSLDSI
                     ALMPCNPSIGGTGKGQLVKEIDALGGQMGLNIDKTYIQSRMLNTAKGPAVHSLRAQAD
                     KFKYHEEMKKTLEDEPNLDIAMDEVVEILHERNVVVGVGTKLGCSFKSKAVILATGVY
                     LNSKIYMGEVAFYEGPNALGYAKYLTDSLVELGLRMRRFKTGTPARVHRDSIDFSVMS
                     LQEGDEKVTPFSFMNENIEKEQEPCYLTRTTEETQKVILDNLKRSAMYSGVIESTGPR
                     YCPSIEDKVVRFSDKTSHQLFIEPEGLNTKEMYIQGISTSLPFEVQLDMYKTIKGLEN
                     CKIMRPAYAIEYDCVDPTQLKISLEIKGVENLFSAGQFNGTSGYEEAAAQGLMAGINA
                     VRKIEGKEPFVLDRSEAYIGVLLDDLVTKGTNEPYRMMTSRAEYRLYLRQDNADMRLT
                     QKGYDIGLVKKDRYERFLNKKAAVEKEFERLKNERVTPKEVNSLLEEKGATPIKVGIS
                     LYEFLKRPEVTYELLEELGKGAGEDVSREVKEQCVIITKYEGYIEKQLKQIDQFKKLE
                     NKKLDEKINYSSIEGLRLEARQKLDDIKPISIGQASRISGVSPADISVLLIHLEQVRR
                     TRGGKGE"
     gene            12628..13347
                     /gene="rsmG"
                     /locus_tag="KRL67_001732"
     CDS             12628..13347
                     /gene="rsmG"
                     /locus_tag="KRL67_001732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HBH1522613.1"
                     /translation="MNNIELLKNGIEGFNIDTNDSMLEKFKIYREILVDWNKKMNLTG
                     IEDEKEVYIKHFLDSISAVKNGYIKNGMSIIDVGTGAGFPGIPLKICLDSLELTLLDS
                     LNKRINFLEEVSRVLELDNVTFIHGRAEDFGKNENYREKYDVATARAVAGLPILMEFC
                     VPFVKVGGYFICLKGPNANLELEESQKAIDVLGLEYIEKIDVELPEIDLNHNILVFKK
                     VMETPVKYPRKAGKPAKNPIK"
     gene            13510..14295
                     /gene="noc"
                     /locus_tag="KRL67_001733"
     CDS             13510..14295
                     /gene="noc"
                     /locus_tag="KRL67_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid occlusion protein"
                     /protein_id="HBH1522614.1"
                     /translation="MEDKRVMEIPIEDVVPNPYQPRKIFSQVSLEELSNSIKVYGIIQ
                     PITVRAKDGKYELIAGERRLRAAKLAELKTIPAIINNMNDESSAVLALLENLQREDLN
                     FIEEAIGYENLIKEHAFTQQQLAEKLGKNQSTVANKLRILKLPNDIKMKLIENNLTER
                     HARAFLKLPSEDLMQSVLDKVIKNELTVKKTEKLIQDVLEETKVQEEPDKKQNIKGAM
                     SIRIYINTIKQAFDAISNTGIDAKYNEIDKGDYMEVVVKIPKK"
     gene            14431..15204
                     /locus_tag="KRL67_001734"
     CDS             14431..15204
                     /locus_tag="KRL67_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HBH1522615.1"
                     /translation="MGKVIAVFNQKGGVGKTTTNVNLSASLGTLGKKILVLDLDPQGN
                     TTSGYGINKNEVENTIYEIMLDGLHIKEAIISTEFENVDVVPSATELSGAEIELTSKT
                     NREYILKNSIKAVIDEYDYIFLDCPPSLGMLTINCLTAVDSVLIPIQCEYYALEGVSQ
                     LMETIKLVKSRLNADIEIQGVVLSMFDGRANLSIQVVEEVKKYFKGSVYTTLIPRNVR
                     LAEAPSHGKPVIYYDKRCRGSVAYLELAEEFIDLEEEEW"
     gene            15206..16069
                     /locus_tag="KRL67_001735"
     CDS             15206..16069
                     /locus_tag="KRL67_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HBH1522616.1"
                     /translation="MENKSKRSNRLGRGLSALIPEIKGETSEKEIVNIDIDKIYPNEV
                     QPRKQFDEEKIKVLSDSIKNYGVLQPIVVKMDENNKYMIIAGERRFRASKLANKNQIP
                     AIIKDIDMKDIMEIALIENLQREDLNSIEEALAYKSLIEHYNVTQEEISEAVGKSRPH
                     ITNTLRLLNLGQDVIEMIDSGRITAGHGKALLRIADKDLQLQIAKKIEEEELSVREVE
                     NIAKKISENKQEEPKKSKPKDVFILDVEDKLRNIFGTKVNISKGKKKGKIEIEYYNDD
                     DLNSIVSMLLE"
     gene            16102..17244
                     /locus_tag="KRL67_001736"
     CDS             16102..17244
                     /locus_tag="KRL67_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HBH1522617.1"
                     /translation="MIYLDNAATTYPKPERVYNAVLDCMKNYCANPGRAGHKLAMRAA
                     REIYDTRENIAKLFNVSNPMNIVFTSNATDSLNLAIKGVLQEGDHVITTSMEHNSVIR
                     PIKALEKMGIENTVVKCDYEGFLDYEDLEKSIKNNTKLIVTTHASNVCGTLIDIKKVG
                     EIAKKHNILFLVDASQTAGVYDIDVNECNIDMLAMPGHKCLFGPQGTGILYVREGLNL
                     NILKEGGTGSKSEEIVQPELFPDKYESGTHNTPGIAGLNQGILFIFERGINNIRQHEE
                     ELCQYMIDKLEEVPDIKIYGPKDSKKRASVIALNIGDMDSGEVTFLLDSDYNIATRSG
                     IHCSPLAHTTLGTLKQGAVRFSIGYFNTKDEIDKAVEALKKISKNK"
     gene            complement(17670..18569)
                     /locus_tag="KRL67_001737"
     CDS             complement(17670..18569)
                     /locus_tag="KRL67_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBH1522618.1"
                     /translation="MSIYGYTIFETVLEQNSFIKAASILNLTPSAVSHAIAKLERDVG
                     FQLFIRNRNGISLTREAEKILPHIRILLHSNEILEQEIDRILRSESGIIRIGTFNSIT
                     INWIPEIMKSFRQKYPNIEIRILQGNYINILEWIETSIVDLAFISNTVLPPHIESIPL
                     HKDRIICVFPKGFVPCNPGYVTIEDIQNMSFVMPKKDYDQEAEKILREYHLNVYSQFH
                     IDTDSAIVAMVEAGFGVAILSELIYKSCVGNVDTYPFVPEHYRVISLAYPNSRYDSFA
                     VQKMKEHIVNYTKEKGFFNFKSF"
     gene            18839..20035
                     /locus_tag="KRL67_001738"
     CDS             18839..20035
                     /locus_tag="KRL67_001738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HBH1522619.1"
                     /translation="MVKFAKRMENMKNSANIVRNLFNSMTDPEIISFGGGAPAVEALP
                     IDIIREISNEVLAKNKRGIEALQYSHPYGLRDLREAVAQKLLEPKGVHASPEDIMIVN
                     GGLETMNLICQVFIDPGDVILVESPTFVHCVEIFEMFQAKCIAVDMDEHGIIPEDIER
                     KIQKHHPKMVYVIPTFHNPTGRTLTTERRKKLAGIGSEYDVLILEDDPYRDLRYSGEE
                     LPPIKAFDKTGHTILANSFSKIFSPGARLGYVLAEHAIIERLLDAKTATNSHTSAISQ
                     VLCAEFFNRGYFDEHLKNIRDIYRERRDVMIESIDKYLPKGTKRVFPDGGLFTWVELP
                     ENINTTELLKEAVEHKAAFVAGEGFFTEGGGKGRNCMRISFGNVPPEKIRIGMERLGQ
                     LIASKM"
     gene            20104..20850
                     /locus_tag="KRL67_001739"
     CDS             20104..20850
                     /locus_tag="KRL67_001739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HBH1522620.1"
                     /translation="MTNKVPNLWNQLESLKKSYKWVDLSREVSPETPHWTGFPAMEVE
                     PFYLLDRGDGFNVNKYTLVTQYGTHVDAPIHFVADRKYLDEYTPMDMVLPLCVIDKSD
                     EVAKNSDYVVTVEDIQAWEKEYGNIPEGAFVAFRSDWSKRPDDSLDNYDSDGNKHFPG
                     WSIEALQYMVEKRNIAAIGHETCDTDAPILSNISGYQAETYILGENRYQVEFLVNLSE
                     LPAIGAIIFVTFPRIHKASGFTARCFAICP"
     gene            20883..21386
                     /locus_tag="KRL67_001740"
     CDS             20883..21386
                     /locus_tag="KRL67_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1097 domain-containing protein"
                     /protein_id="HBH1522621.1"
                     /translation="MSKKAMFVWCVGLGLLDGIYCILCSYLPHIQNYMWIGFISLPIY
                     FCGGAKLEEMPKYFCCAASGIFWGGLTLVVLDIGFISNIHLNMLVFVTIVVCIACFVH
                     LGVLTENVWKGLFSSCPMMFGGFAAIFSQGLAEWFWVLVTLTLGLIFGCIMASISTPI
                     TKWIDKN"
     gene            21634..22461
                     /gene="panB"
                     /locus_tag="KRL67_001741"
     CDS             21634..22461
                     /gene="panB"
                     /locus_tag="KRL67_001741"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011721175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HBH1522622.1"
                     /translation="MKNTIQTFKNAKSEGKKLSMLTAYDYSMAKIMDECNINGLLIGD
                     SLGMVVKGEENTLSVTIDEIIYHTKAVKNGAKNALIVSDMPFLSYHVSIEDAVKNAGR
                     LVKEGGAHAVKLEGGSNVIKQIESIVNAQIPVMGHLGLTPQSVNSFGGFKVQGITSEA
                     AKQLIEDAKLIEKAGAFSIVLEGVPAKISEIITNSISIPTIGIGAGINCDGQILIYQD
                     MLGIFEDFVPKFVKQYANVGDIMKDSIKNYILEVETGAFPQEKHSFSIKESELEKLY"
BASE COUNT         9018 a         2489 c         3950 g         7115 t
ORIGIN      
        1 taataacaaa tttatttact atattaataa tttatataaa tagaaaaatg tatcgatttt
       61 gtgggtatta ggaaatatat atagttgggt atattgtaat acctaggtta atactagcta
      121 gtagcttagt aattattata gtgtttataa aaataattaa ggaaaataag ttaaaatcgc
      181 aaaatgaagt aataaagaat aagctagata tgcagtattc tcattatcta agtattcaag
      241 aatcacacac taaagttaag aagctttatc atgatataaa taatcatata tattgtatag
      301 aaaatctcaa atataaaaat aggaaaattg atgggaatgt aaatatttta aaagatgaaa
      361 ttaaggaatt tagatcaatt tacaatacag gtaatgttat attggatata ataataaatg
      421 aaaaaagtga tgtatgtgaa aaaagaaaaa ttaaattcat ttgtgatatc aatttttcca
      481 aagtgaattt tataaaacaa atagatgtat ctagtatatt tgcaaatact tagataatgc
      541 aatagaagct tgtgataaga tacatgataa aagtattgat aaatatatac gaataaaagg
      601 aactatctca aagtcctatt tcgtaattaa atgtgagaat agtaaaataa attcaattag
      661 aaagaataaa agtttattat taacagataa aaagataaac ttgtacatgg aataaggctt
      721 caaagtatca aatcttctat aaaaaaatat agaggagatt tattatttga agataaaaaa
      781 gacaaattta tattgaatat ttatacacca ttaaatcaaa atattgatag ttgggtcgat
      841 taacctacca gttggggaac atatattgat actaaacata tagggatgtt atcttttaat
      901 agaaaaagat agcgtctttt ttttatgaga aataacgagt tttcaatata taaggaggat
      961 tatattaaga taaaaaaatt ataatagcta tgattatagc tataagctta acgctaggtt
     1021 taataacagg atgcacaaat aaggtttcag atacaaatga aaaaatatat accattgaac
     1081 agatttctag tatcaaggaa gacttgtcaa agaaaatatc agaatcaata gaaaatagtg
     1141 gtcttagagt agttgaaaat aaatcagatg gaagttttgt tttgtctttg ggaaacttgg
     1201 agtatacaaa agaacaacct agccaagttt tacattattc aatgactaag gacaagaagg
     1261 gtacgaagga aatattatca attcaatgtg ttaaagattt ttcttataat gaaaagctgt
     1321 cagaagatga taaattcata atggccattt ataacatata tagatcgttg attcatgtag
     1381 aaataagtga gaaagaattt tttagaaaaa ttgaagaagc atttaataaa ggtgaaggaa
     1441 atgtagagat tgataatttg ggagatataa aaattcaagt taagaaattt gatgataaaa
     1501 ctaagtcttt agaattaaaa cttattaaag aatttaaatt gaagtaggaa gattcactat
     1561 gagaaaatac aaagctattt tattaacttt aattataagc attgtagcca taataggatt
     1621 tattatatac aacaactatt ttataaaatg gaatgaagtt gaaaaatata tagataaata
     1681 tatgacatat caagtagttt tagatgatga tatagagcgt attacaaaag aaacgtatac
     1741 aaaagagaat tatgatggga tactttataa atcattttac aaaaattatc cagattatga
     1801 ataccaatat ttttatagtg gtgattatta tgcactacat gtaaataagg taatgctaca
     1861 agcttatcat gaggaagatg gtagtaagct tcaaaaaata attaagaatt aaaagattta
     1921 aaatatccac caatctactc aatagatgag ttgggtaaat aatttatagt aaaataggag
     1981 gattatatgg ggatttactt aaaaatgtca ctagcatatt taaagaaaaa taaacttaga
     2041 acgctacttt taataatagg agttatgtta ggtgttatgt tgatatttgg acctagtgtt
     2101 ataaaagatt cacaaaatga aaacaatgta gtagcaatac atgaacttta cagtggatac
     2161 catgtagagt ttagtgattt gacacttgat gatgctaaaa agttagaaac tgatgggaaa
     2221 gtatcaaagg gtaattcggt acaaaatcta gggcgtattt cagataaaaa aggaaattca
     2281 tttatattaa cttcatcaaa cgaagagtat ctaaaaagta gatacaacaa aataatagaa
     2341 ggtagactac ctaaaaacaa caatgaaata gtattagaga aaaaagcact agaagcaatg
     2401 aatttaagtg aaggagtagg atctacatta aatttaacaa taaaaaagaa ctataaagat
     2461 actaacggaa gtaatcaatt attttctata aataaagaat ttaaaatagt aggagttatg
     2521 gaaaaaccaa aaggatacta tgatgtggtt tggcaatacg aagcagtaac ttatggcaat
     2581 gatgaaacaa acaacattat acctcatgat gcgattagtt ataatagtat attaagcttt
     2641 aaaagtggat ggagaaatat tgaaggtgaa tgtgattatc ttatgaaaaa atataagtta
     2701 ggacaaaaaa gttttctacc aaatgtacct cttgtaaggg aacttcgtta tgtagaagat
     2761 gataagaatg atccgaatgt ttttaaaaga aatgttttaa taactacagt atctataatt
     2821 ttcatattta atattttcaa tattacacta aatcagtcag taaaagaaat gggactgctt
     2881 agactgatgg gcgcaaagaa aagaaacgta aggtgtatga taatctatca agcattaatt
     2941 ataacaataa taggtatact acttgggtta gtagctgggg ttacatattc atatatagga
     3001 attagtaatt ataacgaagt tttatacagt gaagctggtc ttaaccctaa attatatata
     3061 aataatgaaa tagttataaa agctacattg gttggaataa tttctgtttt tatatcttgt
     3121 attataccaa cttttaaaat cggcaagata tcacctattg aatcaataat aaatacagac
     3181 aaaattaaaa atcacagaag tagacttgga atagacaaac tagcaagcaa aatatttgga
     3241 ttttatggat ttatgggact taaaaatatc ggaagaaata aggttagagc tgctatatct
     3301 atgttgtcta ttgctttagg aggatatata ttcattacta cattctcaag tatgcaagaa
     3361 gaggtcaaca gtaaaattga agatatgcaa aatagatata atataacaat gcaatttgga
     3421 gtaaatgcag acccgcaaat atcaaaatac acagatagtg atgtagagaa tatagaaaaa
     3481 attaagggtg ttaaaagtat acataaaatt caaataacaa atggaatgtt tacttttaat
     3541 aataaggata taaacaaaga atttacaacc tataatggag tagaaaaaaa agagataatg
     3601 gaagataaat tagttttaaa gctttatgat gatgattata tagatgaaaa attaaatgac
     3661 tttgttgaag aaggtagctt agatgatatt aaaaaggaca ctgatggata tccaaatgta
     3721 gcagtatata attactttta tgatatagtt gatgatcata ctataaagcc tatattcaaa
     3781 gaattaaata taggagacat aataactgta aaaattcctg ttgaagaaaa taatacgatt
     3841 gtttataaag agatgaaggt aagagtggct gtaattttaa aggatgaatg gattgctatg
     3901 ggagatggcg attttatgcc agatctagaa attataacaa gtaatattca ctctaaaaac
     3961 ataacaggag aacaaaaata cactcaatta ggcataaatt tagaagatcc atatgatgag
     4021 attgttaata atgagataca gaaaatttca agaagtatac cagggtctct atttaatagt
     4081 agattaagtt atcatgaaat gtcagaaagt atatataaaa gttacattaa atcacaaata
     4141 gcaataattg cactagtttt aataatagca tcaattaata ttttctgtac tataagaaca
     4201 aatctactaa ttagaaaaaa agaaatttca acgcttaggg cattaggtgt tagtcttaaa
     4261 aatatgaaaa aaatgcttat atatgaagca ttagcatatg cagtttttag ctttataata
     4321 gctatgattc catcatttat aaatctagtt aaatttacaa attggaataa tgatgcgtat
     4381 ataaactatg gaatagagaa ttttatgtct tttacattcc caattaagga atctttacta
     4441 ttctttgcta tatctgtatt tgtatgttta atagcagtag ttgtaagtaa tagagatttt
     4501 gaaagtatga atattattga aggtataaaa gagaatgaat aatttgggag gtaaatgtat
     4561 gaagctgtta aaagttatag atattaaaaa aatatatgga aaaggagaaa atgaaataaa
     4621 ggctgttgat ggaattactt tagagataga accccataaa tttagtgcaa taattggaca
     4681 aagtggttct ggaaaaagca cattacttca ctgtatggca gggcttgata aaccaacttt
     4741 aggtgatgta tttatggata atttagattt atatacttta agtgatgaca agctatctaa
     4801 tcttagaagt aaagaatttg ggtttatatt ccaaaactat aatctaatac ctgttatcaa
     4861 tgtttatgat aatatagttc ttcctatttt aattgctaaa ggaaagatag ataataatta
     4921 tattaatgat ttaataacta agttaggatt agaaagtcag attaataaat ttcctaatga
     4981 attatcaggt ggtcaacaac aaagagttgc aatagcaaga tcacttgcca ataagccatc
     5041 aataattttt gcagatgaac ctactggaaa tttagatagt aaaacaactg atgaggtgat
     5101 gaatttacta aagtattgtg taaatgaata caagcagacg ttggttatga taactcataa
     5161 tgatgaaata gctaagatgg ctgattgtat aataactatt agtgatggta aagtcatata
     5221 aaatgcattg tataaaggtt taatcgtggg tattattctt gtgattcttc cccaccattt
     5281 aaatccacga aaaaaggact gtcaaatgag taattttaaa ttgctgtgcg acagttcttt
     5341 ttttacttgt ttttcattaa tttatattta tgcaaaattt gagcttaaaa cggtcataag
     5401 aaataaaggt ataaaaatac cattttaaaa tagaataatt tacaaattgt aagattaaaa
     5461 aatatttttg agaggatgaa tatacatact acatttatta atcattatct tatttgtgaa
     5521 acttagaaat attatgggct aacactacaa gtataaactc tgaaagaaca ttttgattgc
     5581 ctctgcttag aaatctttta aagttaatac tatgttttat ttgggcgaaa gctccttatg
     5641 cctgaatact tctattcatt ctaagttcac atacttgtgt accagtgatt ctttctaaat
     5701 tatcttaatg ccaaaatctt gttcgcattt caaaataaag tcaggtagct actttaacat
     5761 tttaggtata tttcttcaat agtttgtctc caaacaaagg tacatctatt agcaacagat
     5821 ttaattttac ttatatcaat aaataatata tcctttgaaa cttcattaat gtcaccaaga
     5881 taatttataa gttaagctat tgtatactat cttcaccaac tacatcctca atagttttat
     5941 actttcttac ttcagcttgg taatctaaat ctgtaataat tatttctctt tgattttcat
     6001 ctctttgcct acaaaagttt aatatgaata ttattccata attatcaatt ttaagttcaa
     6061 aaaagattgc aggtaagtct gtatttacac aaacctactt atcagtctct ttttttgaaa
     6121 ttaatttagc atgaactata gtacgagcat ctctaaattc atagttataa gtatacaaag
     6181 ttataatttt atctgaaata tctaccttta tatcagattt gtgtactgat ctttaggtta
     6241 tataattcaa tgaatcttta aactcatcat tattaaagtg tatacttaaa taatctctgt
     6301 aaattaagtc agcagtatat attgaaaaaa ttttataagt ataagtttgt actttatatt
     6361 ttgtttctac taagttattt tctttgaaaa gtgcttcatc agtatagttt tctagttgac
     6421 caaacatagt tttatttttc atataatgac catagactat taaactctta tcattttcaa
     6481 agttatttct ataatccatg aaaatattac ctgaaggaag ataattttta taaaaattat
     6541 gagttaggta gaaatcattg ttatatcctt gtactacagg atagtctata tttgtgttgt
     6601 ttattttaag ccagaatcta taatcattat ttttatcata aagttcttga tgtttactat
     6661 tgttattgct agtgttttct ctgagttcag tatatacttc atcagctttt ctatattcag
     6721 ataattttat gtaagccgaa aggctagaga aaattagtgt aataatacaa ataatgttta
     6781 ttaatatttt tactagtatt ttcaataaaa aaaatacata atatatatat ggtagatttt
     6841 gtgatataag cgtcccaaaa actcgttgac aacagtagta aaagtattta taattatatg
     6901 tgttatgttt actttgaggg taaagttatt atttttataa acggaaaata aaatctgaaa
     6961 ggcggtgcaa aataatgagt aaaagaactt atcaaccaaa aaagagacaa agaagtaaag
     7021 aacatggttt cagaaaaaga atgaagacgt ctaacggaag aaacgtgtta aagagaagaa
     7081 gagctaaagg aagaaataga ttaactcatt aattacaaaa gggccgctgc aaaggtggcc
     7141 tttttgtgta aataattaca attcatagct gtgtttatgg aggttaacta tggactttaa
     7201 taggactaaa gggttgaaaa aagactctga ttttagaaaa gtatataaac acggcaaatc
     7261 ttttgcaaac aaatatttag taatatatat acttaaaaat aaatcagatt atagtagagt
     7321 aggtatttct gtttcaaaaa aagtaggaaa agctattact aggaatagag taagaagatt
     7381 aataaaagaa gcctacagat taaatattga tgaaaaaatt aagcctggat atgatatagt
     7441 atttatagca agggtatcta gtaaggatgc tacttttaaa gacatagata agtctataaa
     7501 aaatttagtt aaaagaacgg atatttctat ttaattgtat gctataaata tggaggttaa
     7561 tgactttgaa taaaataatt tatatattaa aggaaataag taagtattta tcgaatttat
     7621 gtatttattt agtgagattt tatcaaaagt atatatctcc cttaaaggga cctacctgta
     7681 gattttatcc aacttgctca caatattcaa tagaggcgtt taagaaatat ggatttgtaa
     7741 aaggtatgta tttaacgata agaagacttt taaaatgtca tccttttcat ccaggaggtt
     7801 acgaccctct aaaataaaag agagtaacta ttaggaggta taaaaaaatt gaatataata
     7861 ggaaatttct taggattgat tcttaaggta atatttgaat ttgttaatca ttatggtatt
     7921 tctataatac tttttacgat acttgtaaag attattttat taccattaac aattaagcag
     7981 actaagtcaa caaaggcaat gcaagatata caacctcgta taaaagagat acaagaaaaa
     8041 tataaaaata agccagaaaa acaaaatgaa gaaattgtta agttatatgg agaagctaaa
     8101 attaatccac tatctggttg tttaccttta ttaatacagt ttcctatatt aataggatta
     8161 tttagcgttt tgagagaacc agttgctcat ggcgtttttg caaacaaagc tgcattttta
     8221 gcagctgaca atgggttttt atggataaaa agcttaacat caccagatta tgtattagca
     8281 gtattttcag gagcaagtgc atatgtcatg caaaaagtaa tgacaccaaa agatcagtta
     8341 caaggctcta tgaaagtaat gacgtatgta atggcaggta tgtctttcta ttggggattt
     8401 atattcccag caggacttac attgtactgg acagtaagta acttattctc aatagctcaa
     8461 tattacttaa taatgaatcc tttaaaagct aaactggctg ctaactctaa ggaggaaaaa
     8521 gttaatgaga aaaagcctaa aattaataaa aagtaagagt aaagaggaag ctataagtaa
     8581 agctatagca gagttgaatt taaaagaaga ggatatagaa gtagaaatac ttgaaaatcc
     8641 aagtaaaggg tttttaggtt ttataggtgc taaagatggt acttatgaaa tttttgttat
     8701 cgagagggaa gtagatgtag ctaaaaattt tatagaggta atgttgaaaa atgcaaatgt
     8761 tgatgccaaa gtcaatgtat ctcaaaaaga taatttaatc atggttgata ttgaaggcaa
     8821 agaagctgca agtttaattg gtagaagagg ggaaacttta gattctattc aatttttgac
     8881 tggattagct cttaataaaa taaataaaga ttctcataca agagtacttg tagatataga
     8941 aaattataga tcaaaacgag aagaatcttt aataagatat gctaataaag ttgctagaga
     9001 agtagcgaaa acaagaaaaa ctaaaaaatt agattatatg aatccatatg agagaagaat
     9061 aatacattca gctcttcaaa atgacaaata tgtaattaca tatagtgaag gaaccgatcc
     9121 atatagaaga ttagtaattg agtgtaaaag ataaagtcaa cctcctaaac attagggggt
     9181 tttatttttt aatattatta aatcattaat gattggttaa atgtaaatct atatgtaaaa
     9241 aattatgaat taagaggtga taaatttgtt tatagatgat acaattgcag caatagctac
     9301 agcacctgga gaaggtggta taggtatact tagaataagt ggagaaagag ctttgaaggt
     9361 tgcagaagaa atctttaaat ctatgtcggg aaaaagtatt gaagaataca ataagagaac
     9421 attaatatat ggaaatatag tggataatga aaatataatt gatgaagttt tattggcata
     9481 tatgaaagga ccaaactcct atacaggaga agatgttata gaaattaatt gccatggtgg
     9541 atttatttcg gtaaagaaga tattagaatt aatactatca aaagatgtta gattagcaga
     9601 ggctggtgag tttactaaaa gagcattctt aaatggaaga atagatttat ctcaagcaga
     9661 ggctgtaatt gatgttataa aagccaaaac tgatatagct catgaagttg ctcaaaatca
     9721 attagagggc tctttatcaa agaagataag agaattgaga gataaagtca ctgagatatt
     9781 agctcatgta gaagttgcta tagattatcc tgaggaagat attgagcata taacatatca
     9841 aactcttaaa gaaaagactg atgaacttaa aaaagaaatt aaaaaattat atgatacagc
     9901 tgaaagtgga aaaatactta gagaaggctt aaagacggta attgttggga aaccaaatgt
     9961 aggtaaatct tcactgctta attctatttt aggagagaat agagcaatag ttactgatat
    10021 accaggaact actagagatg ttatagaaga gtttgtaaac ataaaaggta tacctttaaa
    10081 aattgttgac acagcaggta taagagatac tgatgatatt gttgaaaaaa taggtgttga
    10141 aaagtctaaa gaatcattta ctagtgcaga tttgatagta atggttttag atgcatctag
    10201 aaagttgtca gaagaagata tagagatatt agaaaaactt aaggataaac aaacaatagt
    10261 gttgttaaat aagaatgatt tgaaacagga gattgaagaa gaaaaaatac ttaaatatgt
    10321 agaaaataat agtataataa aaatatcagc tttacaacaa gaaggaatag aagaattaca
    10381 agataaaata gagtctatgg tatataaagg tagtatcaaa aataattcta gtttagtagt
    10441 aacaaattca agacataaag atgcacttag taaagcatat aaatctgcaa cagatgcttt
    10501 aattgccttg gaacaaagca tgccatttga ctttgtagaa gtcgatttaa aaaatatatg
    10561 ggactatctt ggatatatta atggtgatac agtaacagag gatttactag ataatatatt
    10621 ccataatttc tgtattggta agtaatgtac aaaagtaaat ttgtttttat tatagagatt
    10681 tttaaaaaat ttgcagtgtt tctcatgaaa cattaatttt gaaaagaaag gaattataaa
    10741 tgataaagtt tgaagcagga aaatatgatg ttatagtagt cggagcagga catgctggat
    10801 gtgaagctgc tttggctact gcaagaatgg gatataaaac tttgataata acaatgtctt
    10861 tggattccat agcattaatg ccttgtaatc catcaattgg agggactggt aaaggtcaat
    10921 tagttaaaga aatagatgcc ttaggtggtc agatgggact aaatatagac aagacatata
    10981 tacaaagtag aatgttaaat actgccaaag gtccagcagt acactcttta agagcacaag
    11041 cagataaatt taaatatcat gaagaaatga aaaagacatt agaagatgaa ccaaatcttg
    11101 atatagctat ggatgaagtt gtagaaatac ttcatgaaag aaatgttgtg gtaggagttg
    11161 gaactaaact aggatgttca tttaaatcta aagctgtaat attggccact ggagtttatc
    11221 tgaactcaaa gatatatatg ggtgaagttg cattttatga aggtcctaat gcattggggt
    11281 atgcaaaata tcttacagat agcttagttg aacttggtct tagaatgaga agatttaaga
    11341 cgggaactcc agcaagagtt catagagata gtatagactt ctctgtaatg tctttacaag
    11401 agggagatga aaaagtaact ccattctcat ttatgaatga aaatatagaa aaagaacaag
    11461 agccttgtta tttaacaaga actacagaag aaactcaaaa agttatactg gataatttaa
    11521 aaaggtctgc tatgtatagt ggagtaatag aaagtactgg tccaagatac tgtccttcta
    11581 tagaagataa agtagtacga tttagtgata aaacatcaca tcaattattt atagaaccag
    11641 aagggttaaa tactaaagaa atgtatatac aaggaatttc aactagttta ccttttgaag
    11701 ttcaattaga tatgtataaa actataaaag gacttgaaaa ttgtaagata atgagacctg
    11761 catatgctat tgaatatgat tgtgttgacc caactcaact aaaaataagt ttggaaataa
    11821 agggagttga gaacttattt agtgcagggc aatttaatgg aacttctgga tatgaggaag
    11881 cagcagccca aggtcttatg gctggtataa atgcagttag aaaaatagaa ggaaaagaac
    11941 catttgtatt agaccgttca gaagcttata taggtgtctt attagatgac cttgtcacta
    12001 aaggaactaa cgagccttac agaatgatga catcaagagc agaatatcga ctatatttaa
    12061 gacaagataa tgcagatatg agattaactc aaaaaggtta tgatatagga ttagttaaaa
    12121 aagatagata tgaaaggttc ctaaataaaa aggctgctgt agagaaagaa tttgaaagat
    12181 taaaaaatga aagagttaca ccaaaagaag taaatagttt gttagaagaa aaaggagcaa
    12241 caccaataaa ggttggaata tctctatatg aatttttaaa gagaccagag gttacttacg
    12301 aattgcttga agaattaggg aaaggcgctg gagaagatgt ttcaagagaa gttaaagaac
    12361 agtgtgtaat aataactaaa tatgaaggtt atatagagaa acaattgaaa caaatagacc
    12421 aatttaaaaa acttgagaat aaaaagctgg atgagaaaat aaattattca tctatagaag
    12481 gtcttagatt agaggctaga cagaaattag atgatataaa acctatttca atagggcaag
    12541 catctcgtat atctggtgtt tccccagctg atatatcagt tttacttata cacctagaac
    12601 aagtcagaag aactagaggt ggcaaaggtg aataatatag aacttctgaa aaacgggatt
    12661 gaaggtttta atatagatac gaatgacagt atgttagaaa aatttaaaat ttatagagag
    12721 atattagttg actggaataa aaagatgaat cttactggaa tagaggatga aaaagaagta
    12781 tatataaaac attttttaga ctctatttca gctgtaaaga atggatatat aaaaaatggg
    12841 atgtctataa tagatgttgg tacaggcgct ggttttcctg gaataccatt gaaaatatgt
    12901 ctagatagct tagaacttac tttgttagat tcattaaata aaagaataaa ctttttagag
    12961 gaagttagta gagtactaga gttagataat gttacattta tacatggaag agctgaagat
    13021 tttggtaaaa atgaaaacta tagagaaaaa tatgatgttg ctacagcaag agctgttgct
    13081 ggattaccga ttcttatgga attttgtgta ccatttgtaa aagttggagg gtattttatt
    13141 tgcttaaaag gcccgaatgc taatttagag ttagaagaat cacaaaaggc tattgatgtt
    13201 ttaggattgg agtatattga aaaaatagat gtagagttac cagagataga tttaaatcat
    13261 aatatattag tttttaaaaa agtgatggaa acaccagtta agtaccctag aaaagctggt
    13321 aaaccagcca aaaatcctat taaataatga aaaaattatt attggtaagc ttagttattt
    13381 ctcaggaaat aattaagctt accgtaaaaa aacaaaaata aaggaattat taaagtagat
    13441 gcagaaataa atttgtatgt acatatagag aacaaaaata atttatatct aaaataagag
    13501 aggtatgtta tggaagataa aagagttatg gagataccta tagaagatgt agttccaaat
    13561 ccatatcaac caagaaaaat attttcacaa gtttcattgg aggaattgag caattctata
    13621 aaggtatatg gtataattca acctataaca gttagagcaa aagatggcaa atatgaacta
    13681 attgcaggag aaagaagatt aagagcagct aaattagcag agcttaaaac cataccagca
    13741 attataaata atatgaacga tgaatcttct gctgttctag cgttattaga aaatttgcaa
    13801 cgagaagact taaatttcat tgaagaagca attggttatg aaaacttaat aaaagaacat
    13861 gcttttactc aacagcaatt agcagaaaaa ttgggcaaaa atcaatctac tgtagcaaat
    13921 aagcttagaa tattaaagct tccaaacgat ataaaaatga agctaataga gaataattta
    13981 acagaaagac atgctagagc atttcttaaa ctaccaagtg aagatttaat gcaaagtgtg
    14041 ttagacaaag tcataaaaaa tgaattgact gttaaaaaaa cagaaaaatt gatacaagat
    14101 gtattagaag aaactaaagt tcaagaagag cctgataaaa aacagaatat aaagggtgct
    14161 atgagtataa gaatttacat aaatactata aagcaggcct ttgatgctat atcaaataca
    14221 ggaattgatg ctaagtataa tgaaatagat aagggagatt atatggaggt tgttgtaaaa
    14281 atccctaaaa aatagagatt actgcttacc agtaatcttt ttttgtaata taagtcttgt
    14341 aatataaatt gatttataga cattatttat acttaaaaaa cacctattca acatttaaaa
    14401 ttaatttaat tcttaaaggg ggggaacatc atgggcaaag ttatagctgt attcaatcaa
    14461 aagggtggag tcgggaaaac tacaacaaat gtaaatttaa gtgctagctt aggaactttg
    14521 ggaaagaaaa tattagtatt agatttagac ccacaaggaa atacaactag tggatatggt
    14581 attaataaaa acgaagtaga aaatacaatc tatgaaatta tgttagatgg gttacatatt
    14641 aaagaagcaa taataagtac tgaatttgag aatgtagatg tagttccatc agctactgaa
    14701 ctatcaggtg cagagataga gcttacatct aaaactaata gagagtatat tttaaaaaat
    14761 tccataaaag cggtaataga tgaatatgat tatatatttt tagattgtcc accctcactg
    14821 gggatgctta caataaattg tttaactgct gtagatagtg ttttaattcc aatacaatgt
    14881 gaatattatg ctttagaagg tgttagtcaa ttaatggaga ctataaaatt agtaaaatca
    14941 agattaaatg ctgatataga aattcaaggt gttgttttaa gtatgtttga tggaagagct
    15001 aatttatcaa tacaagtagt tgaagaagtt aagaaatact ttaaaggtag tgtatataca
    15061 actctaattc caagaaatgt aagacttgca gaagctccaa gtcatggaaa acctgtaatt
    15121 tattatgata aaagatgtag agggtctgta gcttatttgg agctagcaga agaatttata
    15181 gatttagaag aggaggaatg gtagtatgga gaataagtct aaaagaagta atagattagg
    15241 gagaggtctt agtgcattga taccagaaat taaaggagaa acttcagaaa aagaaatagt
    15301 caacatagat atagacaaaa tatatccaaa cgaagttcaa cctagaaagc aatttgatga
    15361 agaaaagata aaagtattgt ctgactctat aaaaaattat ggtgtcttac agccaatagt
    15421 ggttaaaatg gatgaaaata ataaatatat gataatagct ggagaaagaa gatttagagc
    15481 atcaaaactt gcaaataaaa atcaaatacc tgctataatt aaagatatag atatgaaaga
    15541 tataatggag atagccttaa ttgaaaactt acagagggaa gatttaaatt caatagaaga
    15601 ggctctagca tataagagtt taatagagca ttacaacgta actcaggaag aaatatcaga
    15661 agctgttggt aagagtagac ctcatataac taatacatta agacttttaa atcttggaca
    15721 agatgttata gaaatgatag acagtggtag aattacagca ggacatggta aggctttatt
    15781 gagaattgca gataaagatt tgcaacttca aatagcgaaa aaaatagaag aagaagaact
    15841 ttctgtaaga gaagtcgaaa atatagctaa aaaaatatct gaaaataaac aagaagaacc
    15901 taaaaaatct aaacctaagg atgtatttat tttagatgtt gaagataaac ttagaaatat
    15961 atttggaaca aaggtgaata tttctaaagg aaagaaaaaa ggtaaaatag agatagaata
    16021 ctataatgat gatgatttaa atagtattgt atcaatgctt cttgaataga acaaaaatat
    16081 aaaacagaga ggaagattgt tatgatttat ttagataatg ctgcaactac atatccaaaa
    16141 ccagaaagag tgtataatgc tgttttagat tgtatgaaaa attattgtgc aaacccaggt
    16201 agagcaggcc ataaacttgc tatgagagct gctagggaga tatatgatac aagagagaat
    16261 atagctaagt tgttcaatgt aagcaatcct atgaatatag tatttacatc taatgcaaca
    16321 gattctttaa atttagcgat aaaaggtgta ttacaagaag gagaccatgt aataacaacg
    16381 tctatggagc ataattcagt aataagacct ataaaagcat tggaaaaaat gggtattgaa
    16441 aatactgtag taaaatgcga ttatgaggga tttttagatt atgaagattt agaaaaatca
    16501 ataaagaata atactaaatt gattgtaaca acacatgcgt ctaatgtatg tggaacttta
    16561 atagatataa aaaaagttgg agaaatagct aaaaaacata atatactatt tttagtagat
    16621 gcatcacaaa cagcaggtgt ttatgatata gacgtaaatg aatgtaatat agatatgttg
    16681 gcaatgccag gtcataaatg cttatttgga cctcaaggaa caggtatatt atatgtaaga
    16741 gaagggttga acttaaatat attaaaagaa ggtggaacag gtagtaaatc agaagaaata
    16801 gttcaaccag agttattccc agataaatat gaatcaggaa ctcacaatac tccaggtata
    16861 gcaggtctta accaaggtat actatttata tttgaaagag gaatcaacaa tataagacaa
    16921 catgaagaag aattatgtca atatatgata gacaaattag aggaagtacc agatataaag
    16981 atatatggac caaaggatag taaaaaaaga gcatcagtaa tagctctaaa tataggagat
    17041 atggattctg gagaggttac tttcttatta gatagtgatt ataatatagc aactagatca
    17101 ggaatacatt gttcaccact agcacatacc actttaggta ctttaaaaca aggtgcagta
    17161 aggtttagta taggatattt caacacaaaa gatgagatag ataaagcagt ggaagctttg
    17221 aaaaaaattt caaaaaataa atagcatgag atttaataat atagaaataa aaaatgggga
    17281 tgtttcaaaa taaaaatttg aatagagtct tgaaattttt atgtatattt tctaaataaa
    17341 aacttcaaga ccctattaat taaattacta attttgctaa ttttcataaa atctattaaa
    17401 ttactcataa agttatatat tttcaaaggc ttaagtcttg tatgaaatta cttgttttaa
    17461 ttttttatat aactatgact tttgcaatct ttttagttga aaaagcacct tcaatcatac
    17521 taaaataata agattacaat gctaaattat tttatcctct attataacta taaatacaaa
    17581 ctggtgttcc tcatcttatg aaaaaggtat tgctcagctt tgataaaact gacaactaag
    17641 taataaaaat attatactaa ttttactttt taaaaagact taaaattaaa aaaacccttt
    17701 tcttttgtat aatttacaat atgttctttc attttttgaa ctgcaaaaga atcatatcgt
    17761 gagttgggat aagctaaact aattacacgg taatgctctg gtacaaatgg gtatgtatca
    17821 acattgccaa cacaactttt ataaattagt tctgataaaa tagcaactcc aaaccctgct
    17881 tctaccatgg caacaatagc actatcagta tctatgtgaa attgagagta cacattaaga
    17941 tgatattcac gaagaatttt ttccgcttcc tggtcataat cttttttagg cataacaaag
    18001 gacatatttt ggatatcctc tattgtaaca tacccaggat tacaaggtac aaatcctttt
    18061 ggaaagacac aaatgatacg gtctttatga agcggaatag attcaatatg tggaggaagt
    18121 actgtgttgg atataaaagc taaatccaca atagaggttt caatccattc taggatatta
    18181 atatagttcc cttgaagaat acgtatttca atattaggat atttctgccg aaatgatttc
    18241 ataatctcag ggatccagtt tattgtaata ctattaaaag ttccaatacg aataatacca
    18301 ctttctgaac gtaaaatacg atcaatttct tgttctaaaa tttcgttact atgtaataaa
    18361 atacgaatat gaggtaaaat tttttcagct tctcttgtta aactaatacc atttctattt
    18421 ctaataaaca attgaaatcc tacatctctt tctaattttg caatcgcatg acttacagct
    18481 gacggagtta agtttaaaat acttgctgct tttataaaac tattttgctc taaaactgtt
    18541 tcaaaaatag tatatccata aattgacata tgatttactc ctttccttta gatgagtaaa
    18601 aactcatcta aaacgataaa aagattcgct ttattttata ataataatct actattatta
    18661 aattataaat acaagtgatc atttgcattt tttactcaag ataataaaaa tagtaccacc
    18721 aaaaatacaa gaattaaaat attatttctg tttaaaaatg agcggaaacg gaacctttgc
    18781 tcatctaatc tcatataatt acagacaaat ataaacttga taaaaaggag gaactaatat
    18841 ggtaaaattt gcaaaaagaa tggaaaatat gaaaaattca gcaaatattg ttcgtaatct
    18901 ttttaattcc atgacagatc ctgaaattat ctcttttggt ggaggtgcac cagcagtaga
    18961 ggctttacca attgatatta taagagaaat ttctaatgaa gttttagcaa aaaacaaaag
    19021 agggattgaa gccttacagt atagtcaccc atatggttta agagatttgc gtgaagcagt
    19081 tgctcagaaa ttattagaac caaagggagt acatgcaagt ccagaggaca ttatgattgt
    19141 aaatggtggt ttggaaacaa tgaatctgat ttgccaggta ttcattgatc caggagatgt
    19201 tattttagta gaatcaccaa catttgtaca ttgtgttgaa atttttgaaa tgtttcaggc
    19261 aaaatgcata gcagtagata tggatgaaca tggaatcatt ccagaagata tagaaagaaa
    19321 gattcaaaaa catcacccaa aaatggttta tgtaattcct acttttcata atccaactgg
    19381 taggacattg actacagagc gaagaaaaaa attggcaggt attggtagtg agtatgatgt
    19441 tttgatttta gaagacgacc cttatcgtga tttgagatat agtggagagg agttacctcc
    19501 tattaaagca tttgataaaa caggacatac tattttagca aacagttttt ctaaaatctt
    19561 ttctccagga gcaagacttg gttatgtttt agctgaacat gcaatcattg aacgattact
    19621 tgatgcaaaa acagcaacta attctcatac atcagctatt tctcaagtat tgtgtgcaga
    19681 gtttttcaat cgtggttact ttgatgaaca cttgaagaat attcgagata tttatagaga
    19741 acgcagagat gtgatgattg aaagcattga taagtattta ccaaaaggta ctaaaagggt
    19801 atttccagat ggtggtttat ttacttgggt agagctacct gaaaatatca atacaactga
    19861 attattgaaa gaagcggtag aacataaagc agcttttgtt gcaggagaag gattctttac
    19921 agaaggtggt ggaaaaggac gaaattgtat gagaattagc tttggcaatg tacctcctga
    19981 aaaaattaga attggtatgg aaaggcttgg acaattaatc gcttccaaaa tgtaaataat
    20041 tacttatata aaatacaact ttatatttta agatttataa tataaccaaa ggaggaatta
    20101 cttatgacaa ataaagtacc aaacttatgg aatcaattgg aaagcttaaa aaaatcatat
    20161 aaatgggtgg atttgagtcg tgaagtaagt cctgagacac ctcattggac aggctttcct
    20221 gcaatggaag tagaaccgtt ctacttattg gatagaggag atggatttaa tgtaaataaa
    20281 tatacactag taactcaata tggaacacat gtagatgcac caattcattt tgttgctgat
    20341 agaaagtatt tggatgaata tacgccaatg gatatggtac ttccactttg tgttattgat
    20401 aaaagtgatg aggttgctaa aaattcagat tatgtagtaa ctgttgaaga tattcaagca
    20461 tgggaaaaag aatatggtaa tattccagag ggtgcatttg tggcatttcg ttcagattgg
    20521 tcaaaaagac ctgatgattc tttagataat tatgattcag atggtaataa gcattttcca
    20581 ggatggagta tagaagctct gcaatatatg gttgagaaga gaaatattgc tgcaattgga
    20641 catgaaactt gtgatacaga tgcaccaatt ttatcaaata taagtggata tcaggcagaa
    20701 acatatattt taggagaaaa tcgatatcaa gttgaatttt tagtaaattt gtctgagtta
    20761 cctgcgatag gagctattat ttttgtaaca ttcccaagaa tacacaaagc atctggtttt
    20821 actgcccgtt gttttgcaat ttgcccataa ggttttaact gttagaaggg agggggagac
    20881 agatgagtaa aaaagcaatg tttgtttggt gtgttggctt aggtctacta gatggcatct
    20941 attgtattct ttgttcctac ttaccccata tacaaaacta tatgtggatt ggttttattt
    21001 ctcttccaat ttacttttgt ggaggtgcaa aattagaaga gatgccaaaa tatttttgtt
    21061 gtgcagcaag tggtatattt tggggtgggc taacactagt tgtattagac ataggattta
    21121 tatccaatat acatttaaat atgcttgtgt tcgtaacaat tgttgtatgc attgcttgct
    21181 ttgtacatct aggtgtattg actgaaaatg tatggaaagg attgttctca agttgcccga
    21241 tgatgtttgg tggatttgca gcaatttttt ctcaaggatt agcggagtgg ttttgggtac
    21301 ttgtaacact tacactaggg ttgatttttg gttgtattat ggcctcaatt tcaacaccta
    21361 tcacaaaatg gattgataag aattgaaata aaataattat tcaataattt ttaaaaagtt
    21421 aatttattta tgaaatataa tagtatatta tttcaaaata gtttaaaagg aaaagtatta
    21481 aaaaactttt ccttttaatt taaaacataa atacatttgc cacataagtg cttctttaaa
    21541 atctagtatt atctttacta gatataggag taagttgttt tactaaatta gttttatatg
    21601 aagatattta caaaatttta ggaggaaaaa acaatgaaaa atactataca aacttttaag
    21661 aatgctaaaa gtgaaggaaa aaaactatct atgcttactg cttacgatta ctcaatggca
    21721 aaaataatgg atgagtgtaa tataaatggt cttttaatag gagattcatt aggaatggtt
    21781 gtaaaaggtg aagaaaatac tctctctgtt actatagatg aaattatata tcatacaaaa
    21841 gctgtaaaaa atggggcaaa aaatgcctta atagtaagtg atatgccttt tctatcttac
    21901 catgtttcta tagaagatgc tgttaaaaat gctggtagac ttgttaaaga aggaggagct
    21961 catgctgtaa aattagaggg tggatcaaat gttataaagc aaatagaaag cattgtaaat
    22021 gctcaaatac ctgtaatggg tcatttagga ctgactcctc aatctgtaaa ttcatttggt
    22081 ggatttaagg ttcaagggat tactagtgaa gcagcaaaac aacttattga agatgctaaa
    22141 ttaatagaaa aagcgggggc attttctatt gttcttgaag gtgttcctgc gaaaatatct
    22201 gagataataa ctaactctat ttcaattcct acaataggga taggagcagg aattaattgt
    22261 gacggacaaa ttttaattta ccaagatatg ttaggtatat ttgaagattt cgtacctaag
    22321 tttgtgaagc aatatgcaaa tgtaggagat ataatgaaag attcaattaa aaattacata
    22381 ctagaagttg aaacaggggc atttccacaa gaaaaacata gtttttcaat aaaagaatct
    22441 gaattagaaa aattatacta agattaagga tgttataagc tgtcatatat aacaatttat
    22501 tattatattt agagattgat tatccatctt ttttgggtaa aaatttatcc agttttatga
    22561 ttataataac ca
//
LOCUS       DAEPXF010000053        22348 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain HB27
            SAMN13376389-rid12479423.denovo.057, whole genome shotgun sequence.
ACCESSION   DAEPXF010000053 DAEPXF010000000
VERSION     DAEPXF010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13376389
            Sequence Read Archive: SRR10518432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 22348)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22348)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001069624.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001069624.1
            Genome Coverage       :: 315x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/15/2021 01:54:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,165
            CDSs (total)                      :: 4,076
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 89
            rRNAs                             :: 10, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 10 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 19 of 69
            Pseudo Genes (incomplete)         :: 44 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 10 of 69
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22348
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13376389-rid12479423.denovo.057"
                     /strain="HB27"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="China: Hebei"
                     /collection_date="2017"
     gene            108..224
                     /gene="rrf"
                     /locus_tag="KRL67_001742"
     rRNA            108..224
                     /gene="rrf"
                     /locus_tag="KRL67_001742"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            677..1747
                     /locus_tag="KRL67_001743"
     CDS             677..1747
                     /locus_tag="KRL67_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBH1522623.1"
                     /translation="MADIKLENVTKSYNKKNTVIENLNLTIEDGSFTVLVGPSGCGKS
                     TTLRMIAGLEDITSGKLKIEENDVTKTDASKRDIAMVFQNYALYPHMTVRENIEFGLK
                     NKKVEKEKREKLITEVIDVVGLREYLDVKPGNLSGGQRQRVALARAMVKNPKVFLMDE
                     PLSNLDAKLRNQMRTELIQLHQKLKSTFVYVTHDQVEAMSMADKIVIMNKGKIMQIGS
                     PKKIYQNPKNLFVAQFIGNPCMNILDIKNDVKFGFRPEKATLESINIDGKNDDVFSLF
                     GKVVTKEVLGNETIYCLSVLDSEIRVKTENDIFDIGRTLRISVLEKDLYYFDKNGERI
                     EDLKICKNMYSKIKGDIYEKAI"
     gene            1731..2600
                     /locus_tag="KRL67_001744"
     CDS             1731..2600
                     /locus_tag="KRL67_001744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HBH1522624.1"
                     /translation="MKKQFNKAYLYITPAIIGTLLFIVYPIIKTISLSFKSGSLLSST
                     FENVGLNNYTELLKNPEFIQTITNTAIYTFFMVTISISLAIVLAVWLNKNSIIHNLTQ
                     SIIFTPHVISLVSVAVLWLWIMEPQYGFLNWILSTLHLPTSKWLSSESSALMSLILIG
                     VWKTLGYNILIVLSGLKSIPNYIYEASKLDNANKITNFFKVTLPLLSPTIFFLMITTT
                     TASFQVFDEISIMTQGGPVGSTNMLSYYIYESGFIYYDIGQASAASMFLLAIVMIATY
                     ANFRLLSKKVHYQ"
     gene            2644..3504
                     /locus_tag="KRL67_001745"
     CDS             2644..3504
                     /locus_tag="KRL67_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HBH1522625.1"
                     /translation="MDIIQPIKKDKVDIKKIVSKSMEILILLLLSAIFIFPFIWMLST
                     SFKNPQEMMQLPPTIIPNDIVLENYSTAWHSGPFFRYLLNSVFVTGSVLIVQFLVMVP
                     AAYAFSKIKFKGEKVLFGLLLVGLMIPPQVTFLPVYMMMSKFGFINTYVPLIIPFMTS
                     SFGIFLLRQNFMQISDEIIEAAKLDNASDLKIMFRIMVPMAKPAVITFILFNFIYHWN
                     NYFWPLVMTNTDAIRTLPIGVALLKSSEGITAWNVIMAGNIILILPIILVYIFANKKV
                     KEAFMYSGIK"
     gene            3535..4839
                     /locus_tag="KRL67_001746"
     CDS             3535..4839
                     /locus_tag="KRL67_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBH1522626.1"
                     /translation="MKLKRKLATGLAITLLLGSLVGCSNSNDANASKGEKTDSNITKI
                     DFWAPLGGTNGETAQKMVDQFNSEHKDVQVNMLKQKDYYENATKLQAALTSKDQPDVT
                     LLEITQTGTFASAGALVDMSKYFDKTYQERFFSGLLTNSYYEDKFVGMPFNRSTPILY
                     INKDMATKAGLDPSGPKSWEELKDYASKMTNKSEDTYGFETPIDIWFYEAMVMQSGGE
                     ITNGKKVAFNNEAGQAPVKFWQDMMKAGIMKMPPGEDYNAWDVAKQDFVNGKVGMIFT
                     STADLAGLMQQTEGKFEISTAFLPKNQKYATPTGGANLVMLEGGTDKEKEASAEFIEW
                     MTQTDKIVQFSSSTGYLPTTEDATNSEKLQTLYKEKPQYKVATDQLQYAVALPMLNGY
                     KEATDKLMDEIKKGLTDLNANPKDVVSKGTSAMQAVIDKTNK"
     gene            4902..6164
                     /locus_tag="KRL67_001747"
     CDS             4902..6164
                     /locus_tag="KRL67_001747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HBH1522627.1"
                     /translation="MTKVIVLSVDSLFEKDLEFVKNLPNFKSILENCSIVKNINCVYP
                     TLTYPCHTSMVTGVYPKKHGIYHNEKFDPNKENKDWYWYSKDIKVKTIIDVAKENNLT
                     TSSVLWPVMGANPNIDYNIAEIWAPSREDDPRETFERSSSKVIMDGIYNRHCHYIDWK
                     LEPNMDLFGVNCSVDIIKEYKPDLMLIHLATLDHTRHNYGLFNDEVDKALKMNDKWLG
                     DIIQATKDAGTYEDTNFIILGDHGHLRVDKVINPNVLLKSNGFIKVENGEVKDFDAYV
                     NSSGISAQIIVNNLDRLTELRELLEEFKEELFIENIFTKEEASNLGLEGDFEMVIEGL
                     EGISFGNDFEGSIIKDSDIKDYKFAVATHGHLPTKGNRPPFIAFGPNIKGGMVIEEGD
                     LRAHAATILKMFDLKLDGVEKEAFDFII"
     gene            6351..8321
                     /gene="uvrB"
                     /locus_tag="KRL67_001748"
     CDS             6351..8321
                     /gene="uvrB"
                     /locus_tag="KRL67_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HBH1522628.1"
                     /translation="MDFKIKSDFKPTGDQPEAIKSIVDSINRNEKFSTLLGVTGSGKT
                     FTMANIIQQVKKPTLIMAHNKTLAAQLYSEFKEFFPDNAVEYFVSYYDYYQPEAYVAH
                     SDTYIEKDASINDEIDKLRHSATASILERRDTIIISSVSCIYGLGDPKDYKELMLSIR
                     PGMQRDRDDVIKRLIEIQYERNDINFTRGTFRVRGDILEIFPASNDEKAIRIEFFGDE
                     VDRITEIDYVTGKIVGTRNHVVIFPASHYVTTPERIEKAIVEIEDELQEQIKFFKEND
                     RLLEAQRIEQRTKYDIEMLKEIGFCQGIENYSRHITGRSEGERPYTLMDFFPDDYLII
                     VDEAHVTIPQVRGMYAGDRSRKTSLIENGFRLPSALDNRPLNFQEFEGNINQMLFVSA
                     TPGPYEIQHSETIAEQIIRPTGLLDPIVEVRPINNQIDDLVGEITKTIEKNERVLITT
                     LTKKMSEDLTNYLKEIGIKVKYLHSDIVTLERTEIIRDLRLGKFDVLVGINLLREGLD
                     IPEVSLIAILDADKEGFLRSETALIQTIGRAARNENGRVIMYADRITDSMQNAIDETK
                     RRRDIQNLYNEEHNIIPKTIQKNIRDSIEATKVAEEEVVYGISDTDDKDEIRANIDKL
                     KSEMMEAAQNLQFERAAELRDKVKQLEEKLEK"
     gene            8340..11165
                     /gene="uvrA"
                     /locus_tag="KRL67_001749"
     CDS             8340..11165
                     /gene="uvrA"
                     /locus_tag="KRL67_001749"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HBH1522629.1"
                     /translation="MEDKIIIRGAKEHNLKNIDLELPRDKFIVFTGLSGSGKSSLAFD
                     TIYAEGQRRYVESLSAYARQFLGQMEKPNVEYIEGLSPAISIDQKTTSKNPRSTVGTV
                     TEIYDYLRLLFARVGDVYCPTCGEKISQMTIQEIVDKMLDFPDRTKLQILSPIVRGQK
                     GTHKKVFDNIKKEGFVRVRVNGENYEVSDDIVLNKNQKHNIEVVVDRIVIKDGIESRL
                     ADSIETAVKLSDGLVIAQIIDGEEILFSTKFACPEHGIGIEELSPRMFSFNAPFGACD
                     VCNGLGESKEVDPELVVPNKDLSIKQGAVAAWGSTGVNDDTYYSKMIKSLAEHFNVSL
                     TTPFKDLPDDFVEELLYGKDNIIVEFTYESKFGGTRNYKSYFEGVIVNLERRYRETNS
                     DYMRDKIEEYMAERPCPKCKGMRLKKEVLSVLVDGKNIMEVTNLSVNELIDFMENINL
                     TEKQRFIAHEIIKEIKGRAKFLRDVGLDYLNLSRKAGTLSGGEAQRIRLATQIGSALV
                     GVLYVLDEPSIGLHQRDNDRLITTLRHLTDLGNTLIVVEHDEDTMREADYVIDIGPGA
                     GIHGGEIVAQGTLDEIIENDNSITGQYLSGKKQILLPETTREGNGSFIEIVKASENNL
                     KNIDVKFPLGKFTCITGVSGSGKSTLINDILYKGVASKVNRLKQRAGKHKEIKGIESI
                     DKVINIDQSPIGRTPRSNPATYTGVFDFIRDIFASTNEAKARGYKKGRFSFNVKGGRC
                     EACKGDGIIKIEMHFLPDVYVPCEICKGERYNRETLQVKYKDKTISDILDMNVEEAVE
                     FFENIPNIKRKLETLMDVGLSYIKLGQPSTQLSGGEAQRIKLAAELSKRPTGKTLYIL
                     DEPTTGLHMADVDKLIQVLQRLADTGNTIIVIEHNLDVIKTCDYVIDLGPEGGDKGGT
                     IVATGTPKEVSKVDGSYTGQFLKKYFS"
     gene            11427..13244
                     /gene="uvrC"
                     /locus_tag="KRL67_001750"
     CDS             11427..13244
                     /gene="uvrC"
                     /locus_tag="KRL67_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HBH1522630.1"
                     /translation="MFDIQEHLKKLPSEPGVYLMKDKYDHIIYVGKAISLKNRVRQYF
                     QSSKNHTSKVKSMVKNIYKFEYIITDSELEALILECNLIKRYRPKYNVVLRDDKTYPY
                     IKVTTNEDYPRILKVRRVLKDKAKYFGPYTNITAVNDTLELISSTYPIRSCKIDIDKA
                     IKNKTRPCLNLHINKCLGPCTGNVSKEEYGKMVEEIIMCLSGKEEKLMELLKEKMNES
                     SMNFRFEEAAVYRDKIKSLEEMIQKQKIDATVSDLNQDVVAMARAHNEACVQVFFIRN
                     GKIVGREHFILEGVMDSPRASILSSFVKQFYNEQEYIPKELIIEDEIEDSSILEEWLS
                     SKKGQKVTIRVPQKGEKKSLVEMVRKNAVEYLEKFSDMNKRKYEKSIGALEELKQILS
                     LEKLPIRIEAYDISNIQGVDSIGSMVVYTNAKKDKKEYRRYKIKTVIGPNDYDSMAEI
                     VDRRLKHGNLPDLILLDGGKGQVSAVKKVLELNDVDIPLWGMYKDDKHRTKGLICKEK
                     EIELDKTTNLYRFIASIQEEVHNYAITYHRSLRNKALTKSILDDIQGIGEKRKKSLLN
                     HFKDVDAIKKATMEELLEVDGMNKSIADNVYNFFRKEEN"
     gene            13244..14173
                     /locus_tag="KRL67_001751"
     CDS             13244..14173
                     /locus_tag="KRL67_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr kinase/phosphorylase"
                     /protein_id="HBH1522631.1"
                     /translation="METSNKVSIREIIKELKLKVVYMPDDVDYYVYSQDINRPGLQFA
                     GYFDYFAYERIQIVGKTEYNFFGLMDSHIREEVLDRFFSYEIPALIISRDLEIKPDVI
                     EKAKKYKRILLSTNRNTTRLINRLSNYLDNKLAPHTTIHGVLVDIYGIGVLIKGESSI
                     GKSETALELIQRGNRLVADDAVEIRKLDESMLVGQSPELIRHFLEIRGIGIIDVRSLY
                     GVGAIKNSKMIDLVVHLEAWDETKYYDRLGLDKEYEEILGVNVEKLVVPVKPGRNTAM
                     ILEVAAMNFRQRGMGYDAAQEFTKKLSKLIDTK"
     gene            14387..16183
                     /locus_tag="KRL67_001752"
     CDS             14387..16183
                     /locus_tag="KRL67_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate oxidoreductase"
                     /protein_id="HBH1522632.1"
                     /translation="MGIEYFSKLLESSTEKELLLSKKSNNISTARLISFLIVIAGLAI
                     GFYNKNIVGIFVGVVSIIIFIALLVIHSKVKEEETYFKSKCEVLNKYIKRFGDGWKEF
                     KIDGKEYLKDENSQAKDLDLFGRTSLYQYICVANTSYGKNFLAKYLWNENPDENVILE
                     RQIAIKELLSKDDFSIHIQTLSNIIGKEQKSNSDSSIESFIEYGENKNVYIPKWMHIF
                     TWGLPTATILSFIFCVLGFLPVLPVFLLFIIQLGFSGFGYPRLMQTLSPLFSFSRSIQ
                     VYEKMFEVLEKETFESDYLKELQNKLSKGSGVSRGVKQLNSIGNAVNLRYNQIIYIIA
                     CGVLMWNYHCAEALERWKGIYGNQIRDWFESIGEFEALISLTVISHVKENTCFPVIKD
                     EDTPRLKVEEVYHPLIAEKSVIANSIQLNSQTCIITGSNMSGKTTFLRSIGVNLVLAY
                     AGAPVCAKSFDATCMAIFTSMRIQDDVSQGISTFYAEILRIKSMIQYSLKELPMLVLV
                     DEIFKGTNSADRIIGASEAVKKLSKPWVISMVTTHDFELCDLSGSGDVEVVNYHFSEY
                     YVDDKIHFDYKIKDGRCKTTNAKQLMRMAGIL"
     gene            complement(16323..18104)
                     /locus_tag="KRL67_001753"
     CDS             complement(16323..18104)
                     /locus_tag="KRL67_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron hydrogenase small subunit"
                     /protein_id="HBH1522633.1"
                     /translation="MSLVNLTINGKHVSAPSGTSILDAAKLINIKIHNLCHLHMNEID
                     KLDTCASCRVCMVETERGLVPACGTVIKEGMKVQTNSAKALNARRTIVELLLSDHPQD
                     CFVCEKNGDCELQTIAADLGVRKIRYQGSKSFAGKDTSTKSLVKDHSKCILCRRCETV
                     CNDIQTVGALSGVNRGFNTLVSTFFNSDMVETECTFCGQCISVCPTGALTEVDNVPKL
                     WDVLNKKEKTIVVQVAPAVRVAIGEEFGLEPGSISTGKMVAALKALGFEHVFDTNFGA
                     DFTIMEEATEFIERIQKGENLPILTSCCPAWVNFLEHNYPDKLNLASSCKSPQGMFGS
                     IAKNYYAPKILGINPEELYVVSVMPCVAKKYEASREELSESGILDVDLSITTRELAKM
                     IKEAAIDLPNLQDQDFDNPLGKSTGAASIFGASGGVLEAALRTSYEKITNKTLDNVNF
                     TNVRGLKGIREASIDVDGTTVNVCIVNTLKNARKIMDKVRSGECKYHIIEVMACPGGC
                     VGGAGQPYHHGNTEIVDRRANALYEIDRNKAIRKSHENPDLQAIYKDFFGEPNSDVAH
                     KYLHTHYFDKSCVYGECPQECACEEAK"
     gene            complement(18127..20013)
                     /locus_tag="KRL67_001754"
     CDS             complement(18127..20013)
                     /locus_tag="KRL67_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HBH1522634.1"
                     /translation="MRKVNSFDELKVLADELKPNLSLRKNYSKENVLRRELLVCCDTG
                     CTSSNSLEIVSELENEIKKSGIQDKVSVRLTGCFGFCAQGPIVKVYPDNVFYVKVEPS
                     DAEKIVQSHLIRNTVVEELLYEEESLSKKVEEQEEMSFYKKQLRIALKNCGLIDPDSI
                     EEYIANDGYLALGKCLTSLTPQEVIAEVKTSGLRGRGGAGFPTGSKWEAASKNPAGPT
                     DKKFVVCNADEGDPGAFMDRSVLEGDPHSVLEAMAICGYAIGSDTGYIYIRAEYPKSI
                     ERLKVAIAQAEKYGLLGENILGTGFNFKLELKYGAGAFVCGEGTALMHSIEGRRGEPR
                     MKTYSSSKSGLWKSPTCLNNVETFANIPAIILKGGDWFANLGTEDSSGTKVFALGGKV
                     ENVGLVEVPMGTTLRDIVFEIGGGIPEGKKFKAVQTGGPSGGCIPTEHLDTPIDFGSL
                     SSIGSMMGSGGMLVLDESDCMVDIAKFFLEFTVEESCGKCTPCRIGTTRLLEILTKIT
                     EGNGTLQDLDDLENLAETIQTASLCGLGKAAPNPVLSTLQYFKDEYIAHVVDKKCPAG
                     KCQNLLSYFITDDCKGCTKCSRVCPAGCITGSVKEQHTIDTSKCLKCGACIDNCTFNA
                     IIKK"
     gene            complement(20030..20527)
                     /locus_tag="KRL67_001755"
     CDS             complement(20030..20527)
                     /locus_tag="KRL67_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HBH1522635.1"
                     /translation="MCDFISHNKQLFDELDIFIDSLATKEGALIQVLHEAQGIFGYLP
                     KEVQLHVARKLGVAPAKVYGVVTFYSYFTTEPVGKYKISVCLGTVCFVKGADKILSAF
                     EKQLGIKVGETTSDFKFSLEGLRCLGACGLAPVVTVNGKVYGKVKPDQVSEILDTYRE
                     LELNC"
     gene            complement(20845..22326)
                     /gene="cls"
                     /locus_tag="KRL67_001756"
     CDS             complement(20845..22326)
                     /gene="cls"
                     /locus_tag="KRL67_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HBH1522636.1"
                     /translation="MFDISFFEMSIYEIAVTTIYIINFMVILNLIFREKRNINTTLTW
                     LLILVLIPALGFILYIAFGRNISKNNMFRLKEKDDKIIKSNILDTQVKLQSTSEIDSD
                     IHQHKDMIYALANSNNAHYTNNNDVWIYAESSQFFNSLLEELKKAKKYINIQFYIFKD
                     DKIGTEIIDILIDKAKEGVEVRLLFDAVGGRTLKNSTLSRLKESGVKVGSFFPSFLKI
                     VNFNLNYRNHRKIVVIDGKVGFVGGYNVGDEYLGRNPKFGLWRDTHTKLTGDCVIDLN
                     MRFILDWRYTTKEDLDLEKYFIEEDTNSNHPSENIGIQIVSSGPDISELDEIKYGYLK
                     MIQKARKYIYIQSPYLILDSTFIDTLKIACLSGVDVRVMIPSKPDHPFVYWASYSYAG
                     ELLKFGAKIYTYGQNAFLHAKTIVIDDSICSIGTANMDIRSFELNFEVNAFMYSSKKA
                     LEQRIIFENDILNSKEITLDIYNSRSTYIKIKESISRLLSAVL"
BASE COUNT         8541 a         3023 c         3774 g         7010 t
ORIGIN      
        1 attaagatat atgtgtagtt tttagagtgt taactctaaa gaaactagtg ttactagatt
       61 ctaaaaaatt attaaaaact taatagaaat attaagcata aagattatgt ggttacaata
      121 gcagagagga tacacctgtt cccattccga acacagaagt taagctctct agcgctgatg
      181 gtacttggtg ggaaactgcc tgggagagta ggacgtagcc acgtaatttt tttgagtaaa
      241 tttataaaaa ttaaaaaagt tttgtttttt atgtttagac cttgcaaatt tgggacataa
      301 tataattgta aaacttaata tagcaaaaat aaattactca atctccccca cttaaagacc
      361 tctatccccc atagaggtct tttttatttt caaatcaaat catataaaaa taaatagttt
      421 tatatgaccc cttctaaatt aaaattatat tgtgacattg aatattgact atgaaaagta
      481 taaaaagtta tagctatcat aaacagtata tcatcttaac ttcattatat ttaacaaaaa
      541 atgtttataa tttaaccttt attttaataa gtttactata tattcacttt gttttaatat
      601 atcctttaca aattaagagt attattaatc tatagataaa tcgtataatt aaatcaaata
      661 ttttaggagg ataaaaatgg cagatataaa actagaaaat gtaacaaaat cttataataa
      721 aaaaaataca gttatcgaaa atttaaatct tactatagaa gatggctctt ttacagtatt
      781 ggtaggccca agtggatgtg gtaaatctac tacgttaaga atgattgcag gtcttgaaga
      841 tataacttca ggtaagttaa aaattgaaga aaatgatgta acaaaaacgg atgctagtaa
      901 aagggatata gctatggtat ttcaaaatta tgcattatat ccgcatatga ctgttagaga
      961 aaacatagaa tttggtctta aaaacaagaa agtagaaaaa gaaaaaagag aaaaacttat
     1021 aacagaagtt attgatgtag tagggttgag agaatattta gatgtaaaac caggaaactt
     1081 atctggaggt caaagacaaa gagtggcact tgctagagca atggttaaaa atccaaaggt
     1141 atttttaatg gatgaaccgt tgtcaaattt ggatgctaaa ttaagaaatc aaatgagaac
     1201 agaattaata caattacatc aaaaattaaa atctacattt gtttatgtaa cacatgacca
     1261 agttgaggca atgtcgatgg cagataagat agttataatg aacaaaggaa agataatgca
     1321 aataggttcc ccaaagaaga tatatcaaaa tcctaaaaat ttatttgtgg cgcaatttat
     1381 cgggaatcct tgtatgaaca tactagatat aaaaaatgat gtcaaatttg gtttcagacc
     1441 ggagaaagca acgctagaga gtataaatat agatggaaaa aatgatgatg tatttagcct
     1501 atttggaaaa gttgtgacta aagaagtgtt gggaaatgaa actatatatt gtttatctgt
     1561 tcttgatagc gagataagag tcaaaacgga aaatgatata tttgatattg gaagaacttt
     1621 aagaataagt gtattagaaa aagatttata ttactttgat aaaaatggag aaagaataga
     1681 agatttaaaa atatgtaaaa atatgtattc taaaattaaa ggagatattt atgaaaaagc
     1741 aatttaataa agcatattta tatataacac ctgctattat aggaacttta ttgtttatag
     1801 tctatccaat aatcaagacg atatctctaa gcttcaaatc tggaagtctc cttagttcaa
     1861 cctttgagaa tgtaggctta aacaattata cagaactttt aaagaatcca gaatttatac
     1921 aaactataac aaatactgca atttatactt tttttatggt gactatatct atatcactag
     1981 ccatagtact tgctgtttgg ctaaataaaa attctataat acacaattta acgcaaagta
     2041 taatctttac accacatgta atttcactag tatctgtagc tgtattgtgg ctttggatta
     2101 tggagccaca atatggcttt ttaaactgga tactatcgac attacattta ccaacatcaa
     2161 agtggctttc aagtgaaagt tcagctttaa tgtcactgat tctaatagga gtatggaaga
     2221 ctcttggata caatatctta atagtgttat caggtcttaa atccatacca aactatatat
     2281 atgaagcatc taaattagat aatgcaaaca agataacgaa tttctttaaa gtgacattgc
     2341 cactattatc accaacaata ttttttctta tgataacaac aacaactgca tcttttcaag
     2401 tatttgatga gataagtata atgacacaag gtgggccagt aggttcaact aacatgttat
     2461 cttattatat atatgagtct ggatttatat actatgacat agggcaagca agtgcagcga
     2521 gtatgttttt gttagctata gttatgattg ctacatatgc taactttaga ctcttatcaa
     2581 agaaagttca ttatcaataa agaaaaagta gatattataa gacttatcca aaaggaggag
     2641 aaaatggata ttatacaacc tatcaaaaaa gacaaagttg atataaaaaa aatagtgtca
     2701 aaatcaatgg aaatattaat tctattactt ttatctgcaa tatttatatt cccttttata
     2761 tggatgttat cgacatcctt taaaaatcca caagaaatga tgcaattacc acctaccatt
     2821 ataccaaatg atatagtgtt agaaaattat agtacagcat ggcattcagg tccatttttt
     2881 agatatttgt taaacagtgt atttgtaaca gggagtgttt taattgtaca atttttagtt
     2941 atggttccag cagcatatgc attttctaag attaagttta aaggtgaaaa agtgttattt
     3001 gggcttttgc ttgtaggact tatgattcca ccacaagtaa cttttctacc agtctacatg
     3061 atgatgagta agtttggatt tataaatact tatgttccac ttataattcc atttatgaca
     3121 tcttcatttg ggatatttct gcttagacag aattttatgc agatttcaga cgagattatt
     3181 gaagcagcta aactagataa tgcttctgac ttaaaaataa tgtttagaat aatggttccc
     3241 atggcaaaac cagcagtaat aacatttata ttatttaatt ttatatacca ttggaataat
     3301 tatttctggc ccttagtaat gacaaataca gatgcaataa gaactttacc aataggagtt
     3361 gcgctcctta aatcttcaga aggaattact gcatggaatg taataatggc aggaaatata
     3421 attttaattt taccaataat tcttgtctac atatttgcga ataaaaaagt aaaagaagct
     3481 tttatgtatt caggaataaa ataaaaaatt aaagactaaa tttggaggat gaaaatgaag
     3541 cttaaaagaa aattagcaac aggattagca ataacattat tattaggttc tttagttggg
     3601 tgttcaaatt ctaatgatgc aaatgcatct aaaggagaaa agactgatag caatattacg
     3661 aagatagatt tctgggctcc tttaggtgga actaatggag aaactgctca aaaaatggtt
     3721 gaccaattta atagtgaaca taaggatgta caagtaaaca tgttaaaaca gaaagattac
     3781 tatgaaaatg caacaaaact acaggcagcc ttaacatcta aagaccaacc agatgtaaca
     3841 ttattagaaa taactcaaac aggtacattc gcatcagcag gtgctttagt agatatgagt
     3901 aaatattttg ataaaacata tcaagaaaga ttcttttctg gtcttttaac aaattcatat
     3961 tatgaggata agtttgttgg catgccattt aatagaagta caccaatatt atacataaat
     4021 aaagatatgg caactaaagc aggtcttgac ccttcaggtc caaaatcatg ggaagagctt
     4081 aaggattatg ctagtaaaat gacaaataaa tcagaagata cttatggctt tgaaacacca
     4141 atagatattt ggttctatga agctatggta atgcaaagtg gcggagaaat aactaatggt
     4201 aaaaaagtag catttaataa tgaagcagga caagcaccag ttaagttctg gcaagatatg
     4261 atgaaagctg gaataatgaa aatgcctcct ggagaagatt ataatgcttg ggatgtagca
     4321 aaacaagatt ttgtaaatgg taaggtaggt atgatattta catcaacagc agatttagca
     4381 ggacttatgc aacaaacaga agggaagttt gaaatatcaa cagcattctt accaaaaaat
     4441 caaaaatatg caactcctac aggtggggct aatctagtta tgcttgaagg tggaacagat
     4501 aaagaaaaag aagcaagtgc agaatttata gaatggatga cacaaacaga taaaatcgtt
     4561 cagtttagtt catcaacagg atacttaccg acaactgaag atgcaacaaa ctcagaaaag
     4621 ttacaaacat tatataaaga aaagcctcaa tataaggttg caactgacca attacaatat
     4681 gctgttgcac ttccaatgtt aaatggatac aaggaagcaa ctgataagct aatggatgag
     4741 attaaaaagg ggcttactga tttaaatgca aatccaaaag atgtagtaag caaaggaact
     4801 tctgcaatgc aagcagtaat agataaaaca aataaataat tatatagact tgcaagttga
     4861 ttaaaagtat tcaataatat ataaatgaag aggtaatgta aatgacaaag gttatagtat
     4921 tatcagtaga ttctttattt gaaaaagact tggaatttgt aaaaaatcta ccaaacttta
     4981 aatctatact agaaaattgt tccatagtaa aaaatataaa ttgtgtttat cccactctga
     5041 catatccatg tcatactagt atggttacag gagtatatcc taaaaaacat ggtatatatc
     5101 ataatgaaaa atttgaccca aataaagaaa ataaagactg gtactggtat tcaaaggaca
     5161 taaaagtaaa aactattata gatgtagcaa aagaaaataa tctgactacc tctagtgtat
     5221 tatggcctgt aatgggagca aatccaaata tagattataa tattgcagaa atatgggctc
     5281 ctagtagaga agatgaccca agagaaactt ttgaacgaag ctccagtaag gttataatgg
     5341 acggtattta caatagacat tgtcattata ttgattggaa attagaaccc aacatggatt
     5401 tatttggagt aaactgttca gtagatataa taaaggaata taaaccagat ttaatgttaa
     5461 tacatttagc tacattggac catacacgac acaattatgg cttatttaat gacgaggtag
     5521 ataaagcatt aaaaatgaat gataaatggc taggagatat tattcaggct acaaaggatg
     5581 caggtacata tgaagataca aattttataa tacttgggga ccatggtcat cttagagttg
     5641 ataaagttat aaatccaaat gtcttgctta aatcaaatgg atttataaaa gtcgaaaatg
     5701 gagaagttaa ggattttgat gcctatgtaa attcttctgg aatatcagct caaataatag
     5761 taaataactt agataggtta acagaattaa gagaactgct tgaagaattt aaagaagaat
     5821 tattcataga gaatattttt accaaagagg aagcatccaa ccttggtctt gaaggtgatt
     5881 ttgaaatggt aattgaaggt cttgaaggaa tttcatttgg aaatgatttc gaaggaagta
     5941 ttataaaaga ttcagatata aaagattaca agtttgctgt tgcgacacat ggtcatctac
     6001 ctactaaggg taataggcct ccatttatag cttttggacc aaatattaaa ggtggtatgg
     6061 ttatagagga aggagacctg agggcgcatg cagcaactat attaaaaatg tttgatttaa
     6121 agttagatgg agttgaaaaa gaagcatttg actttattat ataagttgaa taaagcattt
     6181 attttccatg aattatagac ttctattgca aagataggag tcttttttta tgttttagat
     6241 ttaagtaaaa tttaaatggg taaaatattt aaaataactt aaacaatttt ataaaggtat
     6301 gatataataa tacatgttag gaactaatgt tcggaaaaag gagtgatact atggatttta
     6361 aaataaaatc agacttcaaa cctacaggtg accaaccaga agctattaaa tcaatagtgg
     6421 attctataaa tagaaacgag aaattttcga cattattagg tgtaacgggt tcaggaaaaa
     6481 cattcacaat ggcaaatatc attcaacaag ttaagaaacc taccctaata atggctcata
     6541 ataagacact agcagcacaa ctttacagtg agtttaaaga atttttccca gacaatgcag
     6601 ttgaatattt tgtaagttac tatgattact atcaaccaga agcatatgta gctcacagtg
     6661 acacttatat agaaaaagat gcaagcataa atgatgaaat agacaaactt cgacattctg
     6721 ctacagcatc tattttagag agaagagata ctataataat atcttctgtg tcttgtatat
     6781 atggacttgg tgacccgaaa gactataaag aacttatgtt atccataaga ccaggtatgc
     6841 aaagagatag agatgatgta ataaaaaggt taatagagat acagtacgaa agaaatgaca
     6901 taaattttac acgaggtact tttagggtta gaggagatat actagaaata ttcccagcta
     6961 gtaacgatga aaaagcaata agaatagaat tttttggtga tgaggtagat agaataacag
     7021 agattgatta tgtaacaggg aagattgttg gtactagaaa tcatgttgtt atattcccag
     7081 cgtctcacta tgtaacaaca ccagaaagaa ttgaaaaagc aattgtagaa atagaagacg
     7141 agcttcaaga acaaattaaa tttttcaagg aaaatgatag gctcttagaa gctcaaagaa
     7201 ttgaacaaag aactaagtac gatatagaaa tgttaaagga aataggattt tgtcagggga
     7261 tagagaatta ttctagacat attacaggaa ggtcagaagg agaaaggcca tatactttaa
     7321 tggatttctt cccagatgat tatttgataa ttgtagatga ggcacatgtt accataccac
     7381 aagtaagagg tatgtatgca ggagacaggt ctagaaagac atctcttata gagaatggtt
     7441 ttagattacc ttctgcactt gacaatagac cattaaattt tcaagaattt gaaggaaata
     7501 taaatcaaat gttgtttgta agtgctacac caggacctta tgaaattcaa cactcagaga
     7561 caatagcaga acagataata agaccaactg gccttttaga cccaattgta gaagtaagac
     7621 ctataaataa tcaaatagat gatttggtag gagaaataac taaaactata gaaaaaaatg
     7681 aaagagtgct tataaccact ttaactaaaa aaatgagtga ggacttgact aattatctta
     7741 aagaaatagg gataaaagtt aaatatttac actcagatat agttacatta gaaagaacag
     7801 aaataataag agatttaaga ttagggaagt ttgatgtcct tgtaggaata aacttactta
     7861 gagaaggatt ggatatacca gaagtttcac ttatagcaat tttagatgca gacaaagaag
     7921 gtttcttacg ttccgaaaca gccttaatac agactattgg acgtgcagct agaaatgaaa
     7981 atggaagagt aattatgtat gcagacagga taactgactc aatgcaaaat gctattgatg
     8041 aaaccaagag aagaagagat atacaaaatt tatataatga agagcacaat atcattccta
     8101 aaactataca gaaaaatata agagatagta tagaagctac taaggtggca gaggaagaag
     8161 ttgtatatgg aatatctgat acagatgata aagacgaaat aagagctaat atagataaat
     8221 taaaatcaga gatgatggaa gctgctcaaa acttacaatt tgaaagagca gcagaactta
     8281 gagataaagt gaaacaatta gaagaaaaat tggaaaaata gaaaggtaaa aacaacatta
     8341 tggaagataa aattataata agaggagcaa aagagcataa tttaaaaaat atagatttag
     8401 aattaccaag agataaattt attgttttta caggtttgtc agggtctggg aagtcttctt
     8461 tggcatttga tacaatatat gcagaagggc agagaagata tgtagaaagt ctatctgctt
     8521 atgcaagaca atttttaggt cagatggaaa agccaaatgt agaatatata gagggtttat
     8581 caccagctat ctcaattgac cagaagacta cctcaaaaaa cccacgttct actgttggaa
     8641 cagtaacaga aatatatgat tatttgagac tattatttgc aagagtagga gatgtatatt
     8701 gccctacatg tggtgaaaaa ataagtcaga tgactattca agaaatagtt gataaaatgc
     8761 tagattttcc agatagaacg aaattacaaa ttctttcacc aattgttaga ggacaaaaag
     8821 gcacacataa aaaagttttt gataatatca aaaaagaagg atttgtaaga gttagagtta
     8881 atggtgaaaa ctatgaagtt agtgatgata tagtcttaaa taaaaatcaa aaacataata
     8941 tagaagttgt agtggacagg atagtaatta aagatggaat agaatcaaga ctagcagatt
     9001 caattgaaac tgcagttaag ctatctgatg gtcttgtaat agcccaaata atagatggag
     9061 aagaaattct tttttcaact aaatttgcat gcccagaaca tggaattggt atagaagaat
     9121 tatctccaag aatgttttca tttaatgctc cttttggtgc ttgtgatgtt tgtaatggtc
     9181 ttggggaaag taaagaagtt gacccagaat tagtagtacc aaacaaagat ttatcaataa
     9241 aacaaggtgc agtggcagca tggggttcaa caggtgttaa tgatgacact tattacagta
     9301 aaatgataaa aagtttagca gagcatttta atgtatcttt gacaacacca tttaaagatt
     9361 taccagatga ttttgtggag gaattacttt atggtaaaga taatataatt gtagagttta
     9421 cttatgagtc aaaatttgga ggaactagaa attataaatc atactttgaa ggtgtaatag
     9481 taaacttaga gagaagatat agagaaacta attcagatta catgagggat aagattgaag
     9541 aatacatggc agaaagacca tgtccaaagt gtaaaggtat gagacttaaa aaggaagttt
     9601 tatctgtttt agttgatgga aaaaatataa tggaagttac aaacttatct gtgaatgaat
     9661 tgattgactt tatggaaaat ataaatctta cagaaaaaca gaggtttata gctcatgaga
     9721 ttataaaaga gataaaagga agagctaagt tcttaagaga tgttggtctt gattatttaa
     9781 atttatcaag aaaagctgga actctatcag gaggagaagc acaacgtata agattagcta
     9841 ctcaaatagg ttctgcatta gttggagtat tatatgtact tgatgagcca agtatagggc
     9901 ttcaccaaag agataatgat aggcttataa ctactcttag acatctaaca gacttaggaa
     9961 ataccttaat cgttgtagag catgatgaag acacaatgag agaagctgat tatgttatag
    10021 acattggtcc aggtgcaggt atacatggtg gtgaaatagt tgcacagggt actttagatg
    10081 aaataattga aaatgataac tcaataacgg gtcaatattt aagcggtaaa aaacaaatat
    10141 tgcttcctga gactactaga gaaggaaatg gaagttttat agaaatagtt aaagcatcag
    10201 aaaataatct gaaaaatatt gatgtaaagt tcccattagg taagtttaca tgcataacag
    10261 gcgtttcagg ttctggaaaa agtacgctaa taaacgatat actttacaaa ggtgttgcaa
    10321 gtaaagtgaa tagacttaag caaagagctg gtaagcataa ggagataaaa ggaattgaaa
    10381 gtatagataa agttataaat atagaccaaa gtcctattgg aagaacacct cgttcaaatc
    10441 ctgctactta tacaggtgta tttgatttta taagagatat ttttgcatct actaatgaag
    10501 ctaaagcaag aggttacaaa aaaggtagat ttagttttaa tgtaaaaggt ggtagatgtg
    10561 aagcttgtaa gggtgatggg ataataaaaa tagaaatgca tttcttacca gatgtatatg
    10621 ttccttgtga gatttgtaaa ggtgaaagat acaatagaga aacattgcaa gtaaaataca
    10681 aagataaaac tatatctgat atattagata tgaatgtgga agaagctgtt gagtttttcg
    10741 aaaatatacc aaatataaaa agaaaattag aaactttaat ggatgtagga ctttcatata
    10801 ttaaattagg acagccttca actcaattat caggaggaga agcacaacgt ataaaattag
    10861 ctgctgaact tagtaaaaga ccaacaggaa agactttgta tatattagat gaacctacta
    10921 ctggacttca tatggcagat gtagacaagc taattcaagt attacaaaga ctagcagata
    10981 caggaaatac aattatagtc atagaacata atttagatgt tataaagact tgtgattatg
    11041 taatagactt aggaccagaa ggcggagata agggtggaac tatagttgca actgggacac
    11101 caaaagaagt atctaaagtt gatggttctt atacaggtca attcttaaag aaatattttt
    11161 cttaagttat ataaattgtg aaaaatttta gggctagata tatatgataa actgttctgt
    11221 tataagtaaa ttagtcaaat acaagttgta aatagtaatc taaagtaaag tccatattac
    11281 tcttcatggt taaaattgtt ttcaaatcaa ttatagaagg gaatatggac tttaacttgt
    11341 ctaaagcact ggaaattgta taacttttta aatttcatat tttagggtat aattttagta
    11401 taaaatttaa tgttttggag ggcgagatgt ttgatataca agagcattta aaaaaattac
    11461 cttctgagcc cggagtatat ttaatgaaag acaaatacga ccatataata tatgtgggaa
    11521 aagccatatc tctaaaaaat agggtaaggc agtattttca atcttcaaaa aaccatacat
    11581 ctaaagtaaa atctatggtt aagaatatat ataagtttga gtatattata acggattcag
    11641 agttagaggc attaatactt gaatgtaatt taataaaaag atacagacca aagtataatg
    11701 tagtacttag agatgataaa acttatccat atataaaagt aactacgaat gaagattatc
    11761 cgagaatctt aaaagttaga agagttctta aagataaggc aaagtatttt ggaccctata
    11821 ccaatataac tgctgtgaat gatactttgg agcttataag tagtacgtat ccaataagaa
    11881 gctgtaaaat agatatagat aaagccataa agaataagac aagaccttgt ttaaatctgc
    11941 acataaataa atgcttagga ccttgcacag gaaatgtttc taaagaagag tatggaaaaa
    12001 tggtagaaga gattataatg tgtttatctg gtaaagaaga aaaattgatg gaacttttaa
    12061 aggaaaaaat gaatgagagt tctatgaact ttagatttga agaggctgct gtatacaggg
    12121 ataagataaa gagccttgaa gagatgatac agaaacaaaa aatagatgct actgtaagtg
    12181 acttaaacca agatgtggtt gcaatggcaa gagctcataa tgaagcttgt gtccaggtat
    12241 tttttataag aaatggtaag attgtaggta gggaacattt tatattagaa ggtgtaatgg
    12301 atagtccaag agcttctata ttaagttcat ttgtaaaaca attttataat gaacaagagt
    12361 atatacccaa agaattaatt atagaagatg agatagaaga tagctctata ttagaagaat
    12421 ggttgtcatc taaaaagggg caaaaagtaa caataagagt tcctcaaaag ggagagaaaa
    12481 aaagcttagt tgaaatggtc agaaaaaatg ctgtagaata tcttgaaaaa ttttctgata
    12541 tgaataaaag aaaatatgaa aaaagtattg gagcattgga agaattaaag caaatattaa
    12601 gtctagaaaa gttgccaata agaatagagg cttatgatat atcaaatata caaggtgtag
    12661 attcgattgg ttctatggta gtgtatacga atgctaaaaa agataagaag gaatatagaa
    12721 gatataaaat aaagacagtt attggaccta atgattatga ttctatggct gaaattgtag
    12781 atagaagatt aaaacatgga aatttacctg acttgatatt gttggatggt ggaaaaggac
    12841 aagttagtgc agtaaaaaaa gtactagaac ttaatgatgt agatatacct ctttggggaa
    12901 tgtacaaaga tgataagcat agaaccaaag gattgatatg caaagaaaaa gaaatagagc
    12961 tagataaaac aactaattta tatagattta ttgctagtat acaagaagaa gttcataatt
    13021 atgcaataac atatcataga agtctaagaa ataaagcttt gacaaaatct atcttagatg
    13081 atatacaagg aataggtgaa aaaagaaaga aatcattatt aaatcacttt aaagatgtag
    13141 atgctataaa aaaagctact atggaagaac ttttagaagt agatggaatg aataaaagca
    13201 ttgcagataa tgtttataat ttttttagaa aggaagaaaa ttaatggaaa ctagcaataa
    13261 agtatcaata agagagataa taaaagagtt gaagttaaaa gtagtataca tgcctgatga
    13321 tgtagattat tatgtctact ctcaagatat aaatagacca ggattacagt ttgcaggata
    13381 ttttgattat tttgcatatg aaaggataca aatagttgga aagacagaat ataatttttt
    13441 tggattaatg gatagtcata ttcgagaaga agtactagat agattttttt catatgaaat
    13501 accagcgtta ataatttcta gggacttgga aatcaagcca gatgtaattg aaaaggctaa
    13561 aaaatataaa agaatacttt tgagcacaaa tagaaatacc actagactta taaatagact
    13621 gtcaaattat ttggataata agttagcacc acacacaaca attcacgggg tgctagtaga
    13681 tatttatggt ataggtgtac tgataaaagg tgaaagtagt ataggaaagt cagaaacagc
    13741 attagaactg attcaaaggg gtaatagatt agttgcagat gatgctgtag aaataagaaa
    13801 attagatgaa agtatgcttg tgggtcagtc acctgaacta ataagacatt ttcttgagat
    13861 aagaggaata ggaataatag atgttagaag tctatatgga gtaggggcaa tcaaaaattc
    13921 taaaatgata gatttagtag ttcatttaga agcttgggat gaaactaagt actatgatag
    13981 acttggactg gataaagaat atgaagaaat acttggtgtt aatgtagaaa aattggttgt
    14041 acctgttaaa cctggaagaa atactgctat gatattagaa gtagcagcaa tgaattttag
    14101 acaaagagga atggggtatg atgcagctca agaatttact aaaaaattat caaaattaat
    14161 tgatactaag tagtactgaa taatagtgaa ctacacatca tttaattaaa tattgattag
    14221 gattgtgtcc tacaccttat aaattaaaat caagttgtat atatataatg cttattaata
    14281 agatatatta tataaggata atttaaagta caatgctagg ttataataat actagattaa
    14341 ttttatattt gaatttcatt atgtaataag aaagttggaa ttttttatgg gaatagaata
    14401 ttttagtaaa cttttagaaa gtagtactga aaaagaattg ctcttaagca aaaagtcaaa
    14461 taacataagt acagcaagat taatatcttt tttgatagtg atagcagggc ttgctattgg
    14521 attttacaat aaaaatatag taggtatttt tgttggagtt gtatcaataa taatatttat
    14581 agcactctta gttattcata gtaaagtaaa agaagaagaa acatatttta aaagtaagtg
    14641 tgaagtatta aataagtata ttaagcgatt tggagatggc tggaaggaat ttaaaataga
    14701 tggtaaagaa tacttaaaag atgaaaactc gcaagcaaaa gatttagact tatttggaag
    14761 aacatcattg tatcaatata tctgtgtagc taatacatct tacgggaaaa attttttagc
    14821 caaatatttg tggaatgaaa atccagatga gaatgtaatc ttagaaagac agatagctat
    14881 aaaagaactt ttatcaaaag atgatttttc tattcatatt caaacactca gtaatataat
    14941 cggtaaagag caaaaaagca attcagatag tagtatagaa tcgtttatag agtatggaga
    15001 aaataagaat gtgtatatac caaaatggat gcatattttt acttggggtt taccaactgc
    15061 gacaatctta tcttttatat tttgtgtgtt aggattttta cctgttttac ctgttttttt
    15121 actttttata atacagttag gatttagtgg ttttggatat ccaagactta tgcaaactct
    15181 ttctccatta ttttcattta gtagaagtat acaagtatat gaaaaaatgt ttgaggtttt
    15241 agaaaaagag acgtttgaga gtgactattt gaaagaatta caaaataaac tttctaaggg
    15301 aagtggagtt tcaagaggtg ttaaacaatt aaattcaata ggaaatgctg taaacctaag
    15361 gtataatcag ataatatata taattgcctg tggtgttttg atgtggaact atcattgtgc
    15421 agaagctttg gagcgatgga aaggtatata tggaaatcaa attagagatt ggtttgaatc
    15481 tattggggaa tttgaagctc taataagttt aacagttatt tctcatgtaa aggaaaatac
    15541 atgttttcca gtaataaaag atgaagatac tccaagattg aaagttgaag aggtatacca
    15601 tccattaata gcagaaaaaa gtgtcatagc aaattcgata caattaaatt ctcagacttg
    15661 tattatcaca ggttcaaata tgtctggaaa gacaacattt ttaagaagta tcggtgtaaa
    15721 cttagtgctg gcatatgcag gtgctccagt atgcgctaag agttttgatg cgacctgtat
    15781 ggctatattt acttctatga gaatacaaga tgatgtaagt caaggaatat caactttcta
    15841 tgctgagatt cttagaataa aatcaatgat tcaatatagt ttaaaagaac tgccaatgtt
    15901 ggttctagta gatgaaattt ttaagggaac taactctgct gatagaatta ttggagcaag
    15961 tgaggcggta aagaaattat caaaaccctg ggttataagt atggtgacaa cacatgactt
    16021 tgagctatgt gacctttcag gtagcggaga tgttgaagtt gtaaactatc atttctcaga
    16081 gtactatgta gacgataaaa ttcattttga ctataagata aaagatggaa gatgtaaaac
    16141 tacaaatgca aaacaactta tgagaatggc aggtattctt taattaatag ataggttaat
    16201 taataaataa gtctatttag aaatataatt taatttttga aattaataag gattgtctta
    16261 aattacgtaa aagtatttta agacaatcct tttttaagaa taaattttat actttgttat
    16321 gactacttag cttcttcaca agcacattct tgtggacact ctccatagac acaactctta
    16381 tcaaagtaat gagtatgaag gtatttatga gctacatcac tatttggttc tccaaagaag
    16441 tctttataaa tagcttgtaa atcaggattc tcatgagatt tacgaatagc cttatttcta
    16501 tctatttcat ataacgcatt tgcacgtcta tcaactattt cagtattacc atggtgataa
    16561 ggttgaccag cacctccaac acatccacca ggacatgcca taacttctat gatatggtat
    16621 ttacattctc cacttctgac cttatccata atctttctag cgtttttaag tgtatttaca
    16681 atacacacat taactgtagt tccatcaaca tctatacttg cttccctaat tcctttaaga
    16741 cctctaacat tagtgaagtt tacattatct aaagtcttat tagttatctt ctcatatgaa
    16801 gtacgtaggg cagcttctaa aactccacca cttgctccaa atatagatgc agcacctgta
    16861 gattttccta aaggattatc aaaatcttgg tcttgaagat taggtaaatc gatagcagct
    16921 tcttttatca tcttagctaa ctctctagta gttatagaaa ggtcaacatc tagtattcca
    16981 gattcactta attcttctct tgaagcttca tattttttag ctacacaagg cattacagat
    17041 acaacataaa gttcttcagg attgattcct aatattttag gtgcatagta gtttttagct
    17101 atagaaccaa acataccttg aggagactta cagctagatg ctaagtttaa cttgtctgga
    17161 taattgtgct ctaagaagtt tacccatgca ggacaacaac ttgttaatat aggaagattt
    17221 tcaccttttt ggattctttc tataaattca gtagcttctt ccattatagt aaaatctgca
    17281 ccaaagtttg tatcaaatac atgttcaaat ccaagtgctt ttaaagcagc taccattttt
    17341 cctgtagaga ttgaccctgg ttctaatcca aactcttcac caatagcaac tcttacagca
    17401 ggagcaactt gcactactat agtcttttct tttttattta aaacatccca aagtttaggt
    17461 acattatcta cttctgttaa tgctccagtt ggacagacag atatacattg accacagaat
    17521 gtacactctg tttcaaccat atcagagtta aagaatgtac taactaaagt attaaatcca
    17581 cgattaactc cagataatgc tcctactgtt tgtatgtcat tacatacagt ctcacatctt
    17641 ctacataata tacacttaga atggtcttta actaaagatt tagtagaagt atctttacct
    17701 gcaaaagatt tggaaccttg atatcttatt tttcttactc ctaaatcagc agctatagtc
    17761 tgtaattcac aatctccatt tttttcacag acaaaacaat cttgtggatg gtctgataac
    17821 aataattcaa caatagttct acgagcattt aatgctttag cactattagt ctgaactttc
    17881 ataccttcct ttataactgt tccacaagct ggaactaatc ctctctctgt ctcaaccata
    17941 caaacacgac aagaagcaca agtgtcaagc ttatctattt cattcatatg taaatgacaa
    18001 aggttgtgta tttttatatt tattaatttc gcagcatcta agatagaagt tccactaggt
    18061 gctgaaacgt gtttgccatt aatagttaaa ttaactaaac tcattgttat tcctcctcca
    18121 aataagttat ttcttgatta tagcgttaaa tgtacaatta tctatacaag caccacattt
    18181 tagacactta gaagtatcta ttgtatgttg ttccttaaca gaacctgtta tacaaccagc
    18241 aggacacact ctagaacatt ttgtacaacc cttacagtca tctgttataa agtaagaaag
    18301 taagttctga catttaccag caggacattt cttatctaca acatgagcta tatactcatc
    18361 tttgaaatat tgaagagtac ttaaaacagg gtttggagca gctttaccaa gaccacataa
    18421 tgaagcagtt tgtatagttt cagcaaggtt ttctaggtca tctaaatctt gtagagtacc
    18481 attaccttct gtaattttag ttaatatttc taataatctt gtagtaccta tacgacaagg
    18541 tgtacattta ccacaagatt cttcaacagt aaactcaagg aagaacttgg ctatatctac
    18601 catacagtca gattcatcaa gaacaagcat accaccagag cccatcattg aaccgataga
    18661 acttaatgaa ccaaagtcta taggagtgtc taaatgttca gttggtatac atccaccaga
    18721 tggtccacct gtttgaacag ccttaaattt cttaccttca gggattcccc caccaatttc
    18781 aaatacaata tctcttaaag tagtacccat tggaacttca acaagtccta cattttctac
    18841 ttttccacca agagcaaaga ctttagtacc actagaatct tcagtaccta agttggcaaa
    18901 ccaatctcca cccttaagta taattgcagg tatgttagca aaagtctcaa cgttatttaa
    18961 gcaagtagga gatttccata gaccactctt agaagaacta taagttttca ttctaggttc
    19021 accacgtcta ccttcaattg aatgcattag cgctgtacct tcaccacaaa cgaaagcacc
    19081 tgcaccatat tttaattcta atttaaagtt aaatccagtg cctaagatat tttcgccaag
    19141 taaaccgtat ttttcagctt gagctatggc tacttttaat ctttctatag attttggata
    19201 ttctgctctt atgtatatgt atcctgtatc agaacctata gcgtatccac atattgccat
    19261 agcttctagt acactatgag ggtcaccttc aagtacagat ctatccataa atgcacctgg
    19321 gtcaccttca tcagcattac atacaacaaa cttcttatct gttggaccag ctggattttt
    19381 tgaagcagct tcccatttag agccagtagg gaaacctgca ccacctctac cacgaaggcc
    19441 agaagttttt acctctgcaa taacttcttg aggagtaaga gaagttaaac atttgcctag
    19501 agctaaataa ccatcattgg caatatattc ttctatacta tctgggtcta taagaccaca
    19561 attttttaaa gctatacgta actgtttttt gtagaaagac atttcttctt gttcttcaac
    19621 ttttttactt aaagattctt cttcatataa taattcctcg acaactgtat ttctaattaa
    19681 gtggctttga actatctttt cagcatcaga tggctcaact tttacataga aaacgttatc
    19741 aggataaact tttacgatag gaccttgagc acagaaacca aaacaacctg tcaaacgaac
    19801 actaacttta tcttggattc ctgatttctt tatttcattt tctaattcac taactatttc
    19861 tagactgttt gaagatgtac atccagtatc acaacaaaca agtaattctc ttcttaatac
    19921 attttcctta gaataatttt ttcttaagct aagattaggt ttcagctcat cagctaaaac
    19981 ttttaattca tcaaatgaat ttactttacg cattatttat tcacccctct taacaattca
    20041 attctaattc tctataagta tctaatattt cgcttacttg gtcaggtttc actttaccat
    20101 aaactttacc atttacagta acaactggag ctaaaccaca tgcccccaaa catcttaaac
    20161 cttctaaaga aaatttaaaa tcactagtag tttctccaac ttttatgcca agctgttttt
    20221 caaaagcact taagattttg tcagcacctt ttacaaaaca tacagttcct agacagacac
    20281 tgattttata cttacctaca ggttctgtag taaaataaga atagaatgta acaaccccat
    20341 atacctttgc aggagctact ccaagttttc tagcaacatg aagctgaact tcttttggaa
    20401 gatatccaaa tataccttgg gcttcatgta gaacttgtat taacgcacct tcctttgttg
    20461 caagagagtc aataaatata tctagttcgt caaataattg cttgttgtgt gaaataaaat
    20521 cgcacataac aaatccccct tttcttttta aagttattat ttttcataaa ataattaacc
    20581 atgagttgac cccaacatta tgtgggtgaa caaaaccaaa acaataaaga aagaataata
    20641 tacaacaatt tctttattta taaaaacaat gcaaaaaagt tacctattaa aaataaaaac
    20701 tatactagtc tattataata taaaataatt cagaatagtt aaaaaaaata aaaaaaatga
    20761 gctaaattca ataaaaaaaa aataatacat ataaaaaact ttaaaaaata aaccaaaatg
    20821 taaaattttt ataaaataaa agatttataa aactgctgat aatagtcttg aaattgattc
    20881 cttgattttt atataggtag acctagaatt atatatatct aatgttattt ccttagaatt
    20941 taaaatatca ttttcaaata taattctttg ttccaatgct ttttttgaag agtacataaa
    21001 tgcattaact tcgaaattaa gctcaaaact tctgatatcc atattagctg ttcctataga
    21061 gcatatagag tcatctataa ctattgtctt agcatgcaaa aatgcgtttt gcccataagt
    21121 gtagatttta gctccaaact ttaaaagttc tccagcatat gaatatgatg cccagtatac
    21181 aaatggatgg tcaggtttag aaggaatcat aactctcaca tcaacaccag ataaacaagc
    21241 tatttttaaa gtatctataa aagttgagtc aagtattaaa taaggactct gtatgtatat
    21301 atactttcta gctttctgaa tcattttaag gtaaccatat ttaatctcat caagttctga
    21361 tatatctgga ccacttgata ctatctgaat cccaatattt tcagaaggat gattagaatt
    21421 tgtatcttct tctataaagt acttttctaa atctaaatct tctttagttg tatatctcca
    21481 atcaaggatg aatctcatat ttaggtcaat tacgcaatca ccagtaagtt tagtatgtgt
    21541 atctctccat agaccaaatt ttggatttct acctaaatat tcatcaccaa cattatatcc
    21601 acctacaaat cctaccttac catcaatgac aacaattttt cggtgatttc tatagtttaa
    21661 gttaaagttt acaattttca aaaatgatgg gaagaagctt ccaactttaa caccactttc
    21721 tttaagtctg gaaagtgtac tattttttag tgttctacca ccaactgcat caaataaaag
    21781 tcttacctca acaccttctt tagctttgtc tataagaatg tctattattt cagttccgat
    21841 tttgtcatct ttgaaaatat aaaattgtat gtttatatat tttttagctt ttttaagttc
    21901 ttcaagaaga ctgttaaaaa attgactaga ttcagcataa atccacacat cattattatt
    21961 ggtataatga gcattatttg aattagccaa tgcataaatc atatccttat gttgatgtat
    22021 atctgaatca atttcagatg tagattggag tttaacctga gtatctagta tattactttt
    22081 gattatctta tcatcctttt ctttcaatct aaacatattg ttttttgaaa tatttctacc
    22141 aaaagctata taaagtatga atccaagagc aggaatcaat actaatataa gaagccatgt
    22201 taaagttgta tttatgtttc ttttttctct aaagataagg tttagtataa ccatgaaatt
    22261 aattatatat attgtagtaa ctgctatttc atatattgac atttcgaaaa atgatatatc
    22321 aaacataaaa ttccctcctt caaatatt
//
LOCUS       DAEPXF010000054        22284 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain HB27
            SAMN13376389-rid12479423.denovo.058, whole genome shotgun sequence.
ACCESSION   DAEPXF010000054 DAEPXF010000000
VERSION     DAEPXF010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13376389
            Sequence Read Archive: SRR10518432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 22284)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22284)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001069624.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001069624.1
            Genome Coverage       :: 315x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/15/2021 01:54:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,165
            CDSs (total)                      :: 4,076
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 89
            rRNAs                             :: 10, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 10 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 19 of 69
            Pseudo Genes (incomplete)         :: 44 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 10 of 69
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22284
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13376389-rid12479423.denovo.058"
                     /strain="HB27"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="China: Hebei"
                     /collection_date="2017"
     gene            111..656
                     /locus_tag="KRL67_001757"
     CDS             111..656
                     /locus_tag="KRL67_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HBH1522637.1"
                     /translation="MENVITGLSKGAEWFIGLFQKGGEQFIGLVSGTLPTLIVLMVAI
                     NSLIKIIGEERVNNWASKLGKNSFTRYILLPLVSVFFLGNPMCYTFGRFLKEEHKAGF
                     YDAAVSFVHPITGLFPHANAGELFVWLGISAGLTTLGKETTTLALWYFIVGLIVIFIR
                     GIVTEKMYAFLTRKNRATNKA"
     gene            669..1682
                     /locus_tag="KRL67_001758"
     CDS             669..1682
                     /locus_tag="KRL67_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HBH1522638.1"
                     /translation="MEENKILKIEKGTSGWGGPLYIKKEGNRNKILSMTAGGIHEVTL
                     KIKELLGCEIVDGFKTGVSDEEVAVVIIDCGGTARCGVYPKKKIPTINVNPVGKTGPL
                     AKFITEEYYVSDVSPNCISVVDGEDMPQKSQENKSENKSSIRKPDNYNEVKSKAQGEY
                     AKKNIILSIGQGAGQVVSKFYDAGRDTIQMVMNNVIPFMAFVSMLMGIILASGLGDWI
                     ARVISPLAGNIGGLLIISVICTLPFLSPILGPGAVIAQVVGTLVGTQIGLGAIPAYLA
                     LPALFAINGQAGCDFVPVGLSLGEAEPETVEYGVPALFYSRLITGPIAVIIAYGVAVF
                     ALR"
     gene            1904..3877
                     /locus_tag="KRL67_001759"
     CDS             1904..3877
                     /locus_tag="KRL67_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HBH1522639.1"
                     /translation="MSATEKIPSIDRKIFKILIMCSKKEFVSINSIANELNVTTRSVR
                     TYIKQLNKDLGNDIAVIKYIKGQGYKLEIKDEQILNKIIDMNRKNVFSLNSKEDRVEF
                     ILNYLIELDGFITLDSLADEMCVGRTTLVNDFQYVEKVLASYNLNLIKKQNTGMKLNG
                     NELDIRLFILNQLYKNSRKDFSNSKYFKGIKKEEIIKLEEKILTLFKKNNFYVTDEML
                     REVINYIVVLVYRVKEEKKVKDYDVKFDLLKSYDEYFIAREIKHIISGMFECTLNDEE
                     IIYLTIPLVSGNAPASECALNSSRISKNIDELMENIFNQIYVDMGIFINEDELRVGLG
                     YHLSFTLNRLLFNIKLKNVLLEEIKQNYILPFKLAQIAGKVIEKKYNLEVSEDEIGYI
                     AIHFSGYLERNSSRFYSIKKIAIICSTGLGTAKLLKIRVEKLIGNNPKIDTLSSFNLR
                     NINLDEYDIVFTTIDLDTSNINTIVLKINTIFDENKLREQLKTVLCLREGNIDTTNST
                     NLLINNLLDEDKFMILNEKTILASLEKMMDHLMDLGCIDDKFRKNIVNREEKSPTVFD
                     KGLLFPHSVNEKSDKFLMAVGILEEPIEYANRSIKIILMTMFPCENKIDSDLLVKIYE
                     EVLKIGQDVKLTNKISKCRTFLEFKKVLLKNLI"
     gene            3946..4305
                     /locus_tag="KRL67_001760"
     CDS             3946..4305
                     /locus_tag="KRL67_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH1522640.1"
                     /translation="MKLLMILFVAYILNTIFIMKQNKLYFKTLNRAKKKGDIVSTGKK
                     KSYFSKGSIAIISSDLDGFIKFGEILKGRTVMAKFKEIEEIEGLDIYSAEQKFKDEES
                     IVQAIKFIKEKINVSFN"
     gene            4340..4696
                     /locus_tag="KRL67_001761"
     CDS             4340..4696
                     /locus_tag="KRL67_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HBH1522641.1"
                     /translation="MKYEVKISGIGSLVKELMDESNCLIIYDETINDDDLKDISVIHS
                     IATLKSDVEIGDTLTIGNRDYCIVSVGDIAQKTLREIGHCTIKFDGKCEVNLPGEIHV
                     EIGNPDITIGDLITIS"
     gene            4733..6025
                     /locus_tag="KRL67_001762"
     CDS             4733..6025
                     /locus_tag="KRL67_001762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HBH1522642.1"
                     /translation="MLDLNRKLAKLEEEGKKIKVALIGAGHMGNGMVSQMANMKGIEA
                     SIVVDINLELAHKAFTDAGIDEEIIDNVTNEKDAEIKLQEGKVLTCNDFLVACKTKSI
                     DVVIDATGGIAIGAEIALNSILNKKHIVMLNAETDCVVGPILKKLADDAGVIFTGSAG
                     DEPGAVMELFDFADAMGFEVRVIGKGKNNKLDLDCNPDTVREEAERKGASPHMIASFK
                     EGTKTMVEMALMCNATGFVPDVRGGHGIEATVNEVPKKYALKSEGGVLDNYGVVDFVN
                     GIAPGVFVVVAHKLKAVNDELKYLSMGDGPNYILYRPYHLCSLETPLSAAMAVLEHKA
                     TIVPKAGLVAEVMTIAKKDLKKGDFMDGYGAYTCYGTIEKYDVAKAMNAVPIGLISKK
                     TKVVKDIKKGEVITYDMVEIEKDTTLYHLRQLQEKIFG"
     gene            6461..7099
                     /locus_tag="KRL67_001763"
     CDS             6461..7099
                     /locus_tag="KRL67_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBH1522643.1"
                     /translation="MIIIQLNERQLKIIDIVKENEPITSESIASSLNVTRATLRSDLA
                     ILTMTGILDARPKVGYFYSGVSEINLIGKSVKEKTVEDIMSMPVLAKKDESIYDVIVT
                     MFLSDVGSIVIIDENEELCGVVSRKDLLKATIGGSDINKMPIGMIMTRTPNVVTLTKG
                     ASVLLASRKIIEHEVDSIPIVEYKGEDKNHMRVVGRISKTNITKLFLEIVDN"
     gene            7106..7948
                     /locus_tag="KRL67_001764"
     CDS             7106..7948
                     /locus_tag="KRL67_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase/pyrophosphorylase"
                     /protein_id="HBH1522644.1"
                     /translation="MLVKNLIIYAVSDSVGETAQQVAKACMSQFYVNETYEIKRFPYM
                     INKGVLLETLENAKAENALIVYTLVDEELCSIVERYCEREGLSCIDLMTDILREISKR
                     TGRKPKREAGIIRKLDESYFKRVEAIEFAVKYDDGKDPRGILQADIILVGISRTSKTP
                     LSMYLANKNIKVANVPLVPEIPIPKEVFEIDTKKIIGLTNSPEKLNEIRTQRLKALGL
                     SSKANYANLERILQELDYSEEIMKRIGCPVINVSNKAIEETAGIILDIMKENGLKIYK
                     EIEI"
     gene            8009..10636
                     /locus_tag="KRL67_001765"
     CDS             8009..10636
                     /locus_tag="KRL67_001765"
                     /EC_number="2.7.9.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, phosphate dikinase"
                     /protein_id="HBH1522645.1"
                     /translation="METKYVYSFGEGSKDMKSLLGGKGANLAEMTKIGLPVPPGFTIT
                     TEACNDYYVNNESIRAEIIKEIEEHLATLEKDLNKTLGCNKNPLLVSVRSGAVFSMPG
                     MMDTILNLGLNDNSVVGLAEATQNERFAYDSYRRFIQMFSDVAMEVPKYKFENVLDRI
                     KEAKGYTVDTELTTDDLKEIVKEFKAIYKKEIKNDFPQDPKEQLMLAIEAVFRSWNNP
                     RAIVYRKLNDIAHNLGTAVNIQSMVFGNMGETSGTGVAFTRNPATGENKLFGEFLMNA
                     QGEDVVAGIRTPQNISTLADVMPAVFDEFVKITHILEGHYKDMQDIEFTIENERLYIL
                     QTRNGKRTAAAAINVAVDLVEAGIIDEKEAIMRIEPNQLDQLLHPKFEDKALKEAKVI
                     AKGLPASPGAASGKVYFNADDVVKANEKGEKVVLVRLETSPEDIEGMVKAEGILTARG
                     GMTSHAAVVARGMGKCCVAGCGEIKVDEFNKEIRALDDVVIKEGEYISIDGSTGNVYL
                     GDVKKTEVSLTGNFEKLMNWVDKHKCMMVRTNADNPRDARAAIEFGAEGIGLCRTEHM
                     FFDEARLPAVREMILSNTVEQREKALEKILPMQREDFVELFKVMDGKPVNIRLLDPPL
                     HEFLPHDDETIEELSKSMGIKVSDIKKRIVDLDEFNPMLGHRGCRLAITYPEICVMQS
                     KAIIQGAIEAIKAGVKVSPEIMVPLVGEVNELKIIRKMIVETVDAIIKEEGVEVPYTV
                     GTMIEIPRACLTADEIAQEADFFSFGTNDLTQMAFGYSRDDAGKFLGQYVDEEILEKD
                     PFQVLDQNGVGKLVKMGAKLGREVKPELKLGICGEHGGEPSSVEFCYSVGLNYVSCSP
                     FRVPIARLAAAQASIKNPR"
     gene            10850..11095
                     /locus_tag="KRL67_001766"
     CDS             10850..11095
                     /locus_tag="KRL67_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HBH1522646.1"
                     /translation="MTKCPKCGKEVSSRPGSICPRCGFKVADESLKIRCPEPSCRALV
                     SRKLDYCPKCGCKLRGYNISEFVNMVRCKIDETFDKR"
     gene            complement(11384..12682)
                     /locus_tag="KRL67_001767"
     CDS             complement(11384..12682)
                     /locus_tag="KRL67_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HBH1522647.1"
                     /translation="MSKIVAIGCGVGIITANLIPVYADNNTSSINKVDNSILSDSNKR
                     TSEKEILTAREAVSAALKNSEKLKMKSEEIKMLKEKLEVQDEFDSFTGSDNSFPYDQI
                     GLLKNQSEQAKGFMEDQIANDITNKFNDLVSRENELDKIKNNLEIKTKEIKDMKLKKD
                     LGLVTSLETESAELELQTLQNTQKAKLQELKNNQDYFKLLTNIDLNNYQLDKEYRFES
                     FRVGGSVDSYMEGKVNEYLKYDQLILERTEESFNDKDENKADLPDRPNLTRPVAPTKP
                     EQGSLTDDEYKVLMDKYKKDYEKYEKEVEAYNLERQTYAAGLTTYANYLQQKFNTENG
                     LVTLEDSKKALKKGLIDSYAQLLALEDTIQITKKQLDLSEKQLKNTKLRYDLGLITLT
                     DYKKQVVSNEDAKNSYDTLIVNYNSLKNGIEKPWILNTGK"
     gene            complement(12716..15850)
                     /locus_tag="KRL67_001768"
     CDS             complement(12716..15850)
                     /locus_tag="KRL67_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HBH1522648.1"
                     /translation="MNLTQTSVKRPLTIIMVFLVLIVFGGIGYKKMSINLMPDIEIPV
                     VMVMTTWTGAGPQDVDEQVSQKVDESLSAVSNVKSTISSSQESVSMVVAQFEFGTNLD
                     EIMNDVRSKVDALQTSLPDDAAKPTVLKLDMNAQAIGQLVISGGNENSSQALRKYAED
                     VIQPKIESIDGVTSADLKGGEKAQVNVIADPAVLSNYGVSLSTIKGVLSSSNKTFPYG
                     SITQGEDKIVLRAIDKLESLDDIKQIQIPVKGGNTVRLDNLCSVDYGYADKDSIYRYN
                     GKDSLVIDISKQQDANTVKVMQSAYKYVDELNKENPQFKIKIANDTSSYISESINSVM
                     SNLLISAVIAFIVIFAFLKSVRASLVVAVAIPISIIGAIAILYFTGESLNLITASSLV
                     ISVGMVVDNATVVIENIFKYRKSGRLSLDDCAIEGTRTVTNAILASTLTTIAIFLPIM
                     FTEGIAKITFGALGKTLIASLAFSFIGAITLVPSVFAKLSRGKNSQKMQEKDSPIFDK
                     VSEAYKKLLSVCLRHQRLVVISSLLIFVASLFGMGFIGMDFMPAADKGELSISIELPK
                     GLSLESNDYYVSMTEKKVSDIPEIKTLITTLTSDSNNSKASIAIELESQKNRKRTTDE
                     IEKEIIDRVATVPDCKINVSQNSSVMGGAGGADFTLEMKGPDLDVLKILGKEAEKEFS
                     SIKGFRNVETSLTDTTQEAQFTIDKHKAQEYGVNTAEIAGMLRTAINGDSVTTATIDD
                     YKVDVNLKFKADTIDSLEDIKQLKVNSATGKEVPLGAFSDIKMADGLQSISRTDGDFS
                     VNITATLDNLDAGTATKLALEKTNKMVIPKDYSIGVGGETEMMNESMASLGFSMVIAI
                     ALVYMVMVAQFESFSKPFIIMFCVPFAFVGVVLSLLVSGQSLSTVGMLGIILLIGIVV
                     NNGIVLIDYIEQLRKTNRNNDLIGLVSIGCAARLRPVLMTTATTILGMLPTALSLGEG
                     GETMQPLAIVIMGGLTVSTLITLVLIPTIYLIFDKWENKFSDKMPKIFGKITDKIGSI
                     KRKIIKKPKLKAKV"
     gene            complement(15854..17077)
                     /locus_tag="KRL67_001769"
     CDS             complement(15854..17077)
                     /locus_tag="KRL67_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HBH1522649.1"
                     /translation="MKRYLIVFLMCSSLFLVGCGKTEESEPEKPIAVSVQKAVGGEIE
                     NTNSFSGTTKVKDETAVTAQTVGTVQEVYVKLGQNVRKGDELLSISSPELENSVKQSK
                     ASLDLAKASYSSATGGSLEAQVNQAKTALDNAKIQYEESQRNYDNNKILYEQEVISLD
                     QFKKIEFSLEQTKQQLDSAQRAYDTATSKSIPQAKALAKKQLDQAQVSYNLAMSNLDK
                     LTLTSPVDGTITAKNFDSKEMITQSQPAFIISNPNILEVDLNVAESDIGKFKKDGNVD
                     VIIEDQRILGKIDYVPSVVDPQTSLYPVKVLVNNTNNKFKAGMSAQVNLSIEKENGAV
                     TVPKKAIFEENGKKYVYIATKDNIAKKHLVQTGIVTEDKIEIKSGVSDKDTVIIGGIS
                     LISDGTKIFPVEKEK"
     gene            17329..17814
                     /locus_tag="KRL67_001770"
     CDS             17329..17814
                     /locus_tag="KRL67_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HBH1522650.1"
                     /translation="MDNNNILKIADSFLDFLFVLENNIFKESDLLKKFQNNSDIMKAY
                     FEECPMAPSHAKVILYLMTSNSSSISQIASNLGILKSNMTPIIDRLVEHGLVNKFPDP
                     KDRRILRVELTDKAFELFDAVKAILKESLVKKLSNLSEEELTLLDEHTLKLSEIVKKL
                     G"
     gene            complement(17925..20153)
                     /locus_tag="KRL67_001771"
     CDS             complement(17925..20153)
                     /locus_tag="KRL67_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IA DNA topoisomerase"
                     /protein_id="HBH1522651.1"
                     /translation="MKLVLAEKPSVAKTIASFLGAKTRQDGYFEGNDYIITYAVGHLV
                     SLYDMKDYDKDKYSGSWKMNNFPFVPEDKFKFKIDSSKTKQFNTVKKLLNRKDVEYVI
                     NATDNDREGELIAFLIFLLAKNKKPVKRILVNEWTPEDITRGIKNLKDEDEMRNLQAA
                     GYTRLITDWLIGINFTSVATLKYGNGKLLNIGRVILPTVKLVYDRDMEILNFVPKTYY
                     EIEGHFKAEAGEYKGKYVKGKESKFDTLEDANKIIASITSETGKILDKKVTMSKEYAP
                     KLFSLTSLQGYITSKYSNFTSDKVLNVCQSLYEGNGKGGYITYPRTDSIFLEESLASK
                     ASQTLDKLKVGLEYENKIKFSKTKRVFDSSKVDSHSAIIPTYIIPKSLSKDEQLVYDA
                     IKDRFVANFMPPAEYENTEIKTEVDNCTFLTKGKVLKSKGYLEVYNKEEKNDLLPLVN
                     KDDVVDVLEIKPLTKQTTPPKPYTEDTLLKAMKNCGKNVPEEDTTVLSGYSIGTSATR
                     ADVLKKISQVGYVKKKGKSYSITDLGKNLVEIFPVKDLFDVDYTGKLEKSLSDIQKGK
                     YTRKEYLTNIISFIYQNVNIIKQDASKNINTEAYTFDSKTKKFTKTKEKKQKKDVTNA
                     EKEVAISKDSNNKKEVKNDDENPSLGKCPVCQGDVLEFDKGFACKNHKECKFVIWKND
                     KYLSLYKKKVNKTMVKNILKKGETKVKSLTAKNGNKFDALLKYNKNPDTGYFNWKIEF
                     DN"
     gene            20266..20697
                     /gene="dut"
                     /locus_tag="KRL67_001772"
     CDS             20266..20697
                     /gene="dut"
                     /locus_tag="KRL67_001772"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HBH1522652.1"
                     /translation="MYNLKVKKLNDDAIIPNFAHKGDAGMDLYSIEEVVIPSGETKLI
                     KTGICIELPTMTEAQVRPRSGLALKHSVTVLNTPGTIDEGYRGELKIILINHGKNDFK
                     VEKHMKIAQMIVKPIYDISIEEVKELSDSERGKGGFGSTGF"
     gene            20957..22147
                     /locus_tag="KRL67_001773"
     CDS             20957..22147
                     /locus_tag="KRL67_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HBH1522653.1"
                     /translation="MNFNQKRIAASIMATAIIMPTMGNLAYANESEVESVSIESRTIT
                     GNAVNFRKGPGTNHESMGKLYKGDKVEYVGKEGSWVKVKYNGNTGYVHGNYVAINSLG
                     SSNESSDTSVKSTKVVTAKGLNFRTGPSTSSSKISTLGYGTEVGYISESNGWSKISSN
                     GRVGYVSSKYLGTSVNDSTNENAENSSNDLVKGTKVVTAKSLNVRTGPGTSHSKIATL
                     SYGTEVGSISESGGWTKVSYGNQTGYVSSQYLAEKGSVDTSIPSYSTNSPSQGAESVI
                     SFAKTLLGKPYVWGAEGPNSFDCSGFTQYVMKKSAGVSIPRVSRDQSKYGTYVNRGDL
                     RSGDLVFFDTQGSNNGSVSHVGIYIGNGDMIHASSGSAKKVTISNINSSYYSSRYVNA
                     RRVL"
BASE COUNT         8030 a         2900 c         3751 g         7603 t
ORIGIN      
        1 aaaacattta taatattagc atatagtata gaaaaaagat gtaatttgta aaatttatgg
       61 taagcagtaa aattaaaaat ttaaagatat tatagggagg ttataatttt atggaaaacg
      121 ttattacagg tctaagtaaa ggtgcagagt ggtttatcgg tttgtttcag aaagggggag
      181 aacaatttat aggtcttgtt tcaggtactc ttccaacgct aattgtatta atggtagcta
      241 ttaactcttt gattaagata attggtgagg aaagagttaa taattgggct tctaagctag
      301 gaaagaactc ttttacaaga tatattctac ttccattagt atcagtattt ttcttgggaa
      361 atcctatgtg ctatacattt ggtagatttt taaaagaaga acacaaagca ggattttatg
      421 atgcagcagt ttcatttgta catccaatta caggtctttt tccacatgca aatgcaggag
      481 aactttttgt atggttggga atatcagcag gtttgactac tttaggcaaa gaaactacta
      541 cactggcatt gtggtatttt atagttggtc taattgtaat ttttataaga ggtatagtaa
      601 cagaaaaaat gtatgctttt ctaacaagaa aaaatagagc aacaaataag gcttaggagg
      661 tagataaaat ggaggagaat aaaatattaa aaatagaaaa gggaacttca ggctggggag
      721 gtcctttgta tataaaaaaa gaagggaata gaaataagat actatcaatg actgctggtg
      781 gtattcatga agtaacatta aaaataaaag aactactggg ttgtgaaatt gtagatggat
      841 ttaaaactgg ggtttctgat gaagaagtag cagtagttat tattgattgt ggtggaactg
      901 ctagatgtgg agtttatcca aagaaaaaaa tacctacaat aaatgtaaat ccagttggaa
      961 aaacaggtcc tttagcaaaa tttataacag aagaatatta tgtatcagat gttagtccta
     1021 actgtataag tgttgtagat ggggaagata tgccacaaaa atctcaagaa aataaaagtg
     1081 aaaataagag ttctattaga aagcctgata attataatga ggttaagtca aaagcacaag
     1141 gtgaatatgc taaaaagaac ataatacttt ctattggtca aggggcaggg caagtagtat
     1201 caaaatttta tgatgcaggc cgagatacta tccaaatggt aatgaataac gtaataccat
     1261 ttatggcatt tgtaagtatg cttatgggaa ttattttagc atctggactg ggagattgga
     1321 ttgcaagagt tatatctcca cttgcaggta atattggtgg attattgatt atatcagtaa
     1381 tttgtacact tccattccta tcaccaatac taggacctgg tgcagttata gctcaagtag
     1441 tagggactct tgtaggtaca caaattggtc ttggtgcaat accagcatat ttagcacttc
     1501 cagctctatt tgcaataaat gggcaagctg gatgtgattt tgtaccagta ggattaagtt
     1561 taggagaagc agaaccagaa actgtagagt atggtgttcc agctttattt tattctcgtt
     1621 taataactgg tccaatagct gttattattg catatggagt tgcagtattt gcattgagat
     1681 aatatttaat gtcaatataa ggcttggaat taattaataa aattgtaaat ataaatttaa
     1741 gctagtcaag attttttgtt aatgaaagac tagcttttat actattaatt agctgtaatt
     1801 atcgagttag attaatacta aattttcctt tattaaagtg aaattttatg tattaatttg
     1861 gtttcaaatt tgtactagaa tgaaatcaag gaggagatac aaaatgagtg caactgagaa
     1921 aattccttct attgaccgca agatatttaa gatactgatt atgtgtagta aaaaagagtt
     1981 tgtgagtatt aattcaatag caaatgaatt gaatgtcaca actagaagtg taaggacgta
     2041 tataaaacaa ttgaataaag atttaggtaa tgatatagca gtaatcaaat atataaaagg
     2101 tcaaggctat aaattggaaa taaaagatga gcagatactc aataagatta ttgatatgaa
     2161 tagaaaaaat gtattttcat taaattcaaa agaagataga gtagagttta tactaaatta
     2221 cttgatagaa ctagatggat ttattacact tgatagttta gctgatgaga tgtgtgtagg
     2281 aagaactact ttagtaaatg atttccagta tgtagaaaaa gttttggctt catataattt
     2341 aaacttaatt aaaaaacaga atacaggtat gaaattaaat ggaaatgaac ttgatattag
     2401 actgtttata ttaaatcagt tgtataaaaa ctcaaggaaa gatttcagta attcaaagta
     2461 ttttaaagga atcaaaaaag aagaaatcat aaagttagaa gaaaaaatat tgactttatt
     2521 taaaaaaaat aatttttatg ttacagatga aatgttaaga gaagttatta actacatagt
     2581 agttttagtc tatagagtaa aagaagaaaa gaaagtaaaa gactatgatg taaagtttga
     2641 tttacttaaa tcatatgatg aatactttat tgctagggaa ataaaacata taatatcagg
     2701 aatgtttgaa tgcactttaa atgatgaaga aattatttat ttaactatac cattagttag
     2761 tggaaatgca ccagcatcag aatgtgcttt aaatagtagt agaattagta aaaatattga
     2821 tgagttgatg gaaaatatat ttaatcaaat atatgtcgat atgggtattt ttataaatga
     2881 ggatgaatta agagtagggc taggatatca tcttagtttt actctaaaca ggctattatt
     2941 taatataaag ttaaaaaatg ttttgttaga agagattaag caaaattata tacttccatt
     3001 taaattagca caaatagcag gaaaagtaat agagaaaaag tataacttag aagtttcaga
     3061 agatgagatt ggatatatag caatccattt cagtggatat ttagaaagaa atagcagtag
     3121 attctattca ataaaaaaaa tagcaattat atgtagtaca ggtctaggaa cagcaaaact
     3181 attgaaaata agagtagaaa aactgatagg aaataatcct aaaattgata ctctttcaag
     3241 ttttaatttg agaaatatta acttagacga atacgatata gtttttacaa ctattgattt
     3301 agacacatca aatataaata caatagtatt aaaaataaat acaatatttg atgaaaacaa
     3361 actaagagaa caattaaaaa cagtattgtg cctaagagaa gggaatattg acactactaa
     3421 ttctacaaat ttactaataa ataatttgtt agatgaagat aaatttatga tattaaatga
     3481 aaagactata cttgcttcat tagaaaaaat gatggaccat cttatggatt taggatgcat
     3541 tgatgataag ttcagaaaaa atatagtcaa tagagaagaa aaatctccca cagtatttga
     3601 taaaggttta ttatttcctc attctgtaaa tgaaaaatca gacaagtttt taatggcagt
     3661 tggaatttta gaagaaccaa tagaatatgc aaatagaagt ataaaaataa ttcttatgac
     3721 aatgtttcca tgtgagaata agatagactc agacttatta gtaaaaattt atgaagaagt
     3781 tttaaagata ggccaagatg taaaattaac aaataagata agtaaatgta gaactttttt
     3841 agaatttaaa aaggtgttac ttaaaaacct tatataagta ttgtacagca tttatagttg
     3901 ttatacagat ttaaaaataa tgtgattttt ggaaggtgta taaatatgaa attattaatg
     3961 attttatttg ttgcttatat tttaaatact atatttataa tgaagcagaa taaattgtat
     4021 tttaaaactt taaacagagc taaaaaaaag ggtgatatag tgtctacagg caaaaagaaa
     4081 agctattttt ctaaaggaag tatagccatt atatcttcag atttagatgg atttataaag
     4141 tttggagaaa ttttaaaagg gcgtacagta atggctaagt ttaaagagat tgaagaaata
     4201 gaaggattag atatatatag tgcagagcaa aaatttaaag atgaagagag tattgtacaa
     4261 gctataaaat ttataaaaga aaaaataaac gtttctttta attaactcaa tataaatata
     4321 aattatcagg aggaatgtca tgaaatatga agttaagata agtgggatag gcagtttagt
     4381 taaagagtta atggatgaaa gcaattgtct tattatatat gatgaaacca ttaatgatga
     4441 cgacttaaag gatatatctg taattcattc aatagcaaca ttaaaaagtg atgttgagat
     4501 aggagataca cttactatag ggaatagaga ttattgtata gtttcagttg gtgatattgc
     4561 tcagaagact cttagagaaa ttgggcattg tacaatcaaa tttgatggaa aatgtgaagt
     4621 aaatttacca ggagaaatac atgtggaaat aggaaatcca gatataacta ttggagattt
     4681 aataactatt tcttaatttg aaaataaaat tattataaca ggaggaattg gtatgttaga
     4741 tttaaacaga aaacttgcaa aattggaaga ggaaggtaaa aaaataaaag tagctcttat
     4801 aggagctggg catatgggaa atggaatggt aagccaaatg gcaaatatga aaggtattga
     4861 agcttctatt gttgtagata taaatttgga gttggctcat aaagctttta ctgatgcagg
     4921 aatagatgaa gaaattatag ataatgtaac aaatgaaaag gatgcagaaa taaaattaca
     4981 agaaggaaaa gtattgacat gtaatgattt tcttgtggct tgtaaaacta agtcaataga
     5041 tgtagttata gatgcaactg ggggtattgc aattggtgca gagatagcat taaactctat
     5101 tttaaataaa aaacatatag ttatgttaaa tgctgaaact gattgtgttg ttggaccaat
     5161 acttaaaaaa ttagcagatg atgctggagt aatatttaca ggttctgcag gagatgagcc
     5221 aggtgcagta atggaattat ttgattttgc agatgcaatg gggtttgaag ttagagtgat
     5281 aggtaaaggt aaaaataata aattagattt agattgtaat cctgatacag ttagagaaga
     5341 agcagaaaga aaaggagctt cacctcatat gatagcttct tttaaagaag gaactaaaac
     5401 aatggtagaa atggctttaa tgtgtaatgc aactggattt gtgcctgatg taagaggtgg
     5461 tcatggtatt gaagctacag ttaatgaagt acctaagaaa tatgcattaa aaagcgaagg
     5521 tggagtatta gataattatg gtgtagttga ttttgtaaat ggtatagcac ctggagtttt
     5581 cgtagtagta gcccataaat taaaagcagt taatgatgaa ttaaaatatt taagtatggg
     5641 tgatggacct aattacatat tatatagacc atatcattta tgtagcttag agactccttt
     5701 atcagcagct atggcagttt tggaacataa agctacaata gtgcctaaag ctggattagt
     5761 tgcagaagtt atgactatag ctaagaaaga tttgaaaaaa ggtgacttta tggatggcta
     5821 tggagcatat acatgctatg gtactataga aaaatacgat gtagcaaagg ctatgaatgc
     5881 tgtacctatt ggtttgataa gtaaaaagac taaagttgta aaagatatta aaaaaggtga
     5941 agttataact tacgatatgg tagagataga aaaagatact actttatatc atttaagaca
     6001 attgcaagaa aaaatatttg ggtaagattt gatggtaata taaatagata ttagtaaaaa
     6061 gggttgatgg ttatttttaa atcaaccctt ttaaattttt atatattctt gttaggagac
     6121 tttctttaaa agtatattat aggttatttg tagtcataaa aattattttg aattagttct
     6181 gcaattttat attcatattg aaacaatatt attaaaaatt ttaacataaa atgaattaaa
     6241 atgaccaatt atgtattata atattaactg taatactaat atagttctat taattaaaga
     6301 aatatccccc attaatatat aataaatatc tttaagtgag agaacttagt aaaaaatata
     6361 tcgtattcaa taaaaaatct gtttacagaa tggtatatat agttatataa tatactatat
     6421 aagatgcgaa aagataaaaa gtatagcaca atgaaagaag gtgattatta ttcagcttaa
     6481 tgaaagacaa ctaaaaatta tagatatagt taaggaaaat gaaccaataa cgagtgaaag
     6541 tattgcctcc agcttgaatg tgactcgtgc tacacttaga tctgatttag ctatattaac
     6601 aatgacagga atattagacg caagaccaaa agttggatat ttttattcag gagttagtga
     6661 aataaattta attggtaaaa gtgtaaaaga aaaaacagta gaggatataa tgagcatgcc
     6721 tgtactagct aagaaggatg aaagtatata tgatgtaata gtcactatgt ttttatctga
     6781 tgtagggagt atagttatta tagatgagaa tgaagagtta tgtggagttg tttccagaaa
     6841 agacttgcta aaagcaacta taggaggttc tgatataaat aaaatgccaa taggtatgat
     6901 aatgacaaga actccaaatg tagtcacttt aactaaagga gcaagtgttt tattagcttc
     6961 aagaaaaata atagaacatg aagtagattc gattccgatt gttgaatata agggagaaga
     7021 caaaaatcat atgagagttg ttgggagaat atcaaaaacc aacataacta agctattctt
     7081 agaaatagtt gacaattaag gaggtatgct ggtgaaaaac ctaattatat atgcagtatc
     7141 agattcagta ggggagactg cacaacaagt tgcaaaagca tgtatgtctc agttttatgt
     7201 gaatgaaact tatgaaataa aaagatttcc atacatgatt aataaaggag tactacttga
     7261 aactttggag aatgcaaaag ctgaaaacgc tttaattgta tatacattag ttgatgaaga
     7321 attgtgcagt attgttgaaa gatattgcga aagagaaggt cttagttgta tagatttaat
     7381 gacagatata ctaagagaaa taagcaagag aacaggaaga aaacctaaaa gagaagctgg
     7441 tataattaga aaattagatg aatcttactt taagagagtt gaagctatag aatttgcagt
     7501 gaaatatgat gatgggaaag accctagagg tattcttcaa gcagacataa ttttagtggg
     7561 aatatcaagg acatctaaaa ctcctttgag tatgtattta gctaataaaa atataaaagt
     7621 agctaatgtt ccattagttc cagaaatacc aattcctaaa gaagtgtttg agatagatac
     7681 taaaaagata ataggtctta ctaattcgcc tgaaaagctg aatgagataa gaacccaaag
     7741 actaaaagcc ttaggattgt ccagtaaagc aaactacgct aatttagaaa gaatacttca
     7801 ggagttagat tattcagaag agataatgaa aagaattgga tgcccagtaa taaacgtttc
     7861 aaataaggca atagaagaaa cagcaggtat aattttagac ataatgaaag aaaatggtct
     7921 aaagatatac aaagaaatag aaatttaata atgtctatta ataaaaatga tataaattaa
     7981 aataaaaaga ttaatggggg atttaacaat ggaaactaag tatgtttata gttttggtga
     8041 aggaagcaaa gacatgaaat ctttactagg aggtaaaggt gccaatttag cagaaatgac
     8101 taagataggc ttacctgtcc ctcctggatt cactataaca acagaagcgt gtaatgatta
     8161 ttatgttaac aatgaaagta taagagcaga gataataaaa gaaatagaag agcatttagc
     8221 tactttagaa aaagacttaa ataaaacatt aggatgcaac aagaatccat tattagtatc
     8281 agttcgttct ggtgcagtat tctctatgcc aggaatgatg gatacaattc ttaacctagg
     8341 tcttaatgac aatagtgttg ttggcttagc agaggctaca caaaatgaaa gatttgcata
     8401 tgatagttac agaagattca tacaaatgtt ttctgatgtt gctatggaag ttccaaaata
     8461 taaatttgaa aatgtattag atagaataaa agaagcaaaa ggatacacag ttgatacaga
     8521 acttacaact gatgatttaa aagaaatagt aaaagaattt aaagcaattt ataaaaaaga
     8581 aataaaaaat gatttcccac aagacccaaa agagcaatta atgcttgcaa tagaggctgt
     8641 atttagatca tggaataatc ctcgtgcaat cgtataccgt aaattgaatg atatagctca
     8701 taacttagga acagcagtaa atattcaatc aatggtattt ggtaatatgg gagaaactag
     8761 tggtacaggt gttgcattta ctagaaatcc agctacagga gaaaataaat tatttggtga
     8821 atttttaatg aatgcacaag gtgaagacgt tgttgcaggt ataagaactc ctcaaaatat
     8881 atcaactctt gcagatgtaa tgccagctgt attcgatgaa tttgtaaaaa ttactcatat
     8941 acttgaaggt cactataaag atatgcaaga tatagagttt actatagaaa atgaaagatt
     9001 atatatacta caaactagaa atggtaagag aacagcagca gcagcaataa atgtagcagt
     9061 tgatttagtt gaagctggaa taattgatga aaaagaagct atcatgagaa ttgagcctaa
     9121 tcaattagac caattattac atccaaaatt tgaagataag gcattaaaag aagccaaggt
     9181 tatagcaaaa ggtttaccag catcaccagg tgcagcgagt ggtaaagttt atttcaatgc
     9241 tgatgatgta gtaaaagcaa acgaaaaagg agaaaaagta gttttagtta gactagagac
     9301 atctccagaa gatatagaag gaatggttaa agcagaagga atccttacag ctagaggtgg
     9361 aatgacatca catgcagcag ttgttgctag aggtatgggt aaatgttgtg ttgctggatg
     9421 tggagaaata aaagttgatg agtttaataa agaaataaga gcacttgatg atgtagttat
     9481 aaaagaaggt gaatacattt ctatagatgg ttctacagga aatgtatacc taggagatgt
     9541 taagaaaaca gaagtatctt taactggaaa ctttgaaaaa ttaatgaact gggtagacaa
     9601 acataaatgt atgatggtta gaactaatgc agataatcct agagatgctc gtgcagctat
     9661 agagtttggt gcagaaggta taggtctatg tagaactgag catatgttct ttgatgaagc
     9721 tagattgcca gcagttagag aaatgatatt atctaataca gtagaacaaa gagaaaaagc
     9781 tcttgaaaaa atattaccaa tgcaaagaga ggattttgta gagttattta aagttatgga
     9841 tggaaaacca gttaatataa gactactaga cccaccacta catgaatttt tgcctcatga
     9901 tgatgagact atagaagaac tttcaaaatc tatgggaata aaagtaagtg acattaagaa
     9961 gagaatagtt gatttagacg agtttaaccc aatgcttgga cacagaggtt gtagacttgc
    10021 aataacttat cctgaaatat gtgtaatgca atctaaagct ataatacaag gtgctataga
    10081 agcaatcaaa gcaggtgtta aagtttctcc agagataatg gttccattag ttggagaagt
    10141 aaatgagtta aaaataataa gaaaaatgat agtagaaact gttgacgcta taataaaaga
    10201 agaaggtgta gaagttcctt atactgtagg tactatgata gaaataccta gagcttgttt
    10261 aactgctgat gagatagcac aagaagcaga cttcttcagt tttggtacta atgacttaac
    10321 tcaaatggca tttggatatt caagagatga tgctggtaaa ttcttaggtc aatatgtaga
    10381 tgaagaaata ttagaaaaag acccattcca agtactagac caaaatggtg ttggtaagtt
    10441 agttaagatg ggagctaaat taggtagaga agttaaacca gaattaaaac taggaatatg
    10501 tggagagcat ggaggagaac catcttcagt agagttctgt tatagtgtag gacttaacta
    10561 tgtatcttgt tcaccattta gagtgccaat tgctagactt gcagcagctc aagcatctat
    10621 aaaaaatcct agataataat agactgcaaa atatgtaact aaataaaaat aaaatcccct
    10681 attgttcaat gtatcaaatt aagtatttgt aaattgaata taggggattt ttattataaa
    10741 aatttaactt atatagttgt aagtgaaacg tatttaatta tattgcatat attattgtag
    10801 agagaaattt caactatcaa ttaaattgag tttactggag gggataaagt tgacaaagtg
    10861 tcctaaatgt ggaaaggaag tatcatcacg tccaggtagt atatgcccac gatgtggatt
    10921 taaagtagca gatgagagtc taaaaataag atgtccagaa cctagttgta gggctttggt
    10981 atctaggaaa ctggattatt gtcctaaatg tggatgcaaa cttagaggat ataatatttc
    11041 tgaatttgtt aacatggtga gatgtaaaat agatgagaca tttgataaaa gatagttaaa
    11101 cttagagtta agattttgag ggtgtctcaa aatgaacttt ttagttcata aggcactctt
    11161 tttgtatgga atcaaatata tttttataat ttaaacaata aaaaagaggc ttatctctat
    11221 tttgagagag cctctataaa agtatacagt tttataagta aatatatttt tgttaatata
    11281 tttctattat ttgccttctg aatcttccat gattaaagtc acgagtgtgc atagctattt
    11341 tttaatcaaa tgtatgttaa agtaatcttt ctatttacca gaattacttt ccagtattaa
    11401 gtatccaagg tttttctata ccatttttta gtgagttata attcacaata agagtgtcat
    11461 agctattttt agcatcttca ttacttacaa cttgtttttt ataatcagtt agagtaatta
    11521 atcctaaatc atatcttaat ttagtatttt ttaattgttt ttctgataaa tcaagttgtt
    11581 ttttagtaat ctgaattgta tcttctaaag caagtaattg agcatagcta tcaattaacc
    11641 cttttttaag agctttttta ctgtcttcta atgttactag accattttca gtattaaatt
    11701 tttgttgaag ataatttgca taagtagtaa gaccagcagc ataagtctgt ctttcaagat
    11761 tgtatgcttc aacttctttt tcatatttct cgtagtcttt tttatactta tccataagaa
    11821 ctttgtattc atcatcagtt agactacctt gctctggttt tgtaggagca actggtcttg
    11881 taaggttagg tctatctggt aaatctgctt tgttctcatc tttatcatta aaactttctt
    11941 ctgtacgttc caaaatcaat tggtcgtatt ttaaatattc attaacctta ccttccatat
    12001 atgaatcaac tgagccacct actctaaaag attcaaatct atattcttta tctaattgat
    12061 aattatttaa atctatatta gtcaataatt taaaatagtc ttgattgttt tttagttctt
    12121 gaagtttagc tttttgagta ttttgtagtg tttgaagttc aagttcagct gattcagttt
    12181 ctaaagatgt taccagccct aaatcttttt ttaatttcat atctttaatt tctttagtct
    12241 ttatctcaag attgtttttt atcttatcta gctcattttc tcttgaaact aaatcgttaa
    12301 atttattagt aatatcattt gctatttggt cctccataaa gcctttagcc tgttcactct
    12361 gatttttaag aagtccaatt tggtcgtatg gaaacgagtt gtcacttcca gtaaaactat
    12421 caaactcatc ttgtacttct aacttttctt ttaacatttt tatttcttct gatttcattt
    12481 ttaatttttc actattttta agtgcagcac taacagcttc tctagcagtc aaaatttcct
    12541 tttcagatgt tcttttattt gagtcactta aaatagaatt atcaactttg tttatagaag
    12601 aagtattatt atctgcgtaa actgggatta aattggcagt tattatacca acaccacagc
    12661 caattgcaac tattttgctc attcttttat tcaatacaat caccctccat tctatttaaa
    12721 ctttagcttt caattttggt ttctttatta ttttacgttt tatacttcct attttatctg
    12781 ttatcttacc aaatatttta ggcattttgt ctgaaaactt attttcccat ttatcaaata
    12841 ttaagtatat agtaggtata agcaccaaag ttataagtgt agatacagtt agaccaccca
    12901 ttataactat ggctagtggt tgcatagttt ctccaccttc accaagagat agtgctgttg
    12961 gcaacatacc taaaattgtt gttgctgtag tcattaagac tggtcttaat cttgctgcac
    13021 aaccaattga aaccaaacca attaaatcat tgttacgatt tgtttttcta agttgctcta
    13081 tgtagtcaat aagaactata ccattattaa caacaatacc gataagtaag attataccta
    13141 gcataccgac agtacttaaa ctttgaccac taactagaag cgaaagcaca actccaacga
    13201 atgcaaatgg aacacagaac atgattataa atggtttact aaatgattca aactgagcta
    13261 ccattaccat ataaactaat gctatagcta tgaccattga aaatccaaga cttgccatag
    13321 attcattcat catttctgtt tcaccaccaa cacctataga gtaatcttta ggaattacca
    13381 ttttattagt tttttctagc gctagttttg tagcagtacc agcatctaaa ttgtcaagtg
    13441 tagcagttat atttacagaa aaatctccat cagtcctaga tatagattgt agaccatctg
    13501 ccatttttat atctgaaaaa gcaccaagag gaacttcttt tccagttgca gagtttactt
    13561 ttagttgctt tatatcttct aagctatcaa tagtatctgc tttaaatttt aggtttacat
    13621 ccactttgta atcatcaata gtagcagtag tgacggagtc accatttatt gctgtacgta
    13681 acatgcctgc aatttcagca gtatttacac catattcttg agccttatgt ttatctatag
    13741 taaattgagc ttcttgtgtt gtatctgtaa gagaagtttc aacatttcta aatcctttta
    13801 tagagctaaa ttctttttca gcttcttttc ccagtatttt taatacatct aaatcagggc
    13861 ctttcatttc taatgtgaag tctgctcctc cagctccacc cataacacta ctgttttgac
    13921 ttacatttat tttacaatct ggtacagtag ccactctatc aattatctct ttttctattt
    13981 catcagtagt tctttttcta tttttttgag attctaattc aattgctata cttgctttac
    14041 tattatttga atctgatgtc aaagttgtta ttaaagtttt aatttctgga atatctgaga
    14101 cttttttctc agtcatagat acatagtaat cattggattc taggctaaga ccttttggaa
    14161 gttctataga aatagataat tcacccttgt ctgctgcagg cataaagtcc atacctataa
    14221 atcccattcc aaataaggaa gctacaaaaa taagtaaact tgatattaca actaagcgtt
    14281 gatgtctcaa acatacactt aataattttt tatatgcttc acttacctta tcaaatatag
    14341 gactatcttt ttcttgcatc ttttggctat tttttcctct gcttaatttt gcaaatacac
    14401 taggaacgag tgtaattgca cctataaagg agaatgctag ggaagctatt agtgttttac
    14461 ctagtgcccc aaaagttatt ttggctatac cttctgtaaa catgataggt aagaatattg
    14521 ctatagtggt caatgttgat gcaagtattg catttgttac agttcttgtt ccctcaatag
    14581 cacaatcatc aagactcaat ctgccacttt tcctatattt aaatatattt tcaattacaa
    14641 ctgtagcatt atcaacaacc ataccaactg aaattaccaa tgaacttgca gtaataagat
    14701 ttaaactttc accagtaaag tagagaattg ctatagcacc tattatagaa attggtattg
    14761 caactgcaac aactagtgaa gctcttacac tttttaggaa agcaaatatt acaataaatg
    14821 cgataacagc acttataagc aagttgctca ttacgctgtt tattgattca cttatgtatg
    14881 aacttgtatc attagcaatt ttaattttaa attgaggatt ttccttattc aattcatcta
    14941 cgtatttgta tgcactctgc ataactttta cagtgttggc atcttgttgt ttagaaatat
    15001 ctattaccaa actgtccttt ccattgtatc tataaatact atctttgtct gcatatccat
    15061 aatctacact acaaagatta tctaatctaa ctgtatttcc tccttttaca ggaatttgaa
    15121 tctgttttat atcatctagg gattctaatt tatcaattgc tcttaaaact attttatctt
    15181 caccttgagt aattgaacca taagggaatg ttttattact agaggataag acacctttta
    15241 tagtggataa actaactcca taatttgaaa gaacagctgg gtcagctatt acattaacct
    15301 gtgctttttc tccaccttta aggtctgctg aagtaacacc atctatggat tctattttag
    15361 gctgaattac atcttcagcg tattttctta aggcttgtga actattttca tttcctccag
    15421 atatgacaag ttgacctatg gcctgtgcat tcatatccag ctttaaaact gttggtttag
    15481 ctgcatcatc tggaaggctg gtctgtaatg catcaacttt agacctaaca tcattcatta
    15541 tttcatccaa attagttcca aattcaaact gagcaactac catagaaaca ctttcttgtg
    15601 aacttgatat agtagatttt acatttgaaa cggcagataa actttcatca actttttgac
    15661 taacttgttc atcaacgtct tgaggaccag cacctgtcca agtagtcatg accattacaa
    15721 ctggtatttc tatatcaggc attagattta ttgacatttt tttatatcca ataccaccaa
    15781 aaactattaa tactaaaaag accattatga ttgtgagagg acgtttaacg gaagtctgtg
    15841 ttaaattcat agtctacttc tccttttcta caggaaatat cttagttccg tcagaaataa
    15901 gacttatccc tccaataatc actgtatctt tatcacttac accactttta atttctattt
    15961 tatcctctgt tacaattcca gtttgaacca agtgtttctt tgctatattg tcttttgttg
    16021 ctatgtatac atatttttta ccattttctt caaatattgc ttttttaggc actgtaacag
    16081 caccattttc tttttcaata cttaagttta cttgtgcaga catacctgcc ttaaatttgt
    16141 tgtttgtatt atttacaagt actttaacag gatataaaga tgtctgaggg tcaactactg
    16201 aaggtacata atctattttt cctaaaatcc tttggtcttc aattataaca tctacatttc
    16261 catctttctt aaatttacct atatctgatt ctgctacatt taaatcaact tctagtatat
    16321 taggattaga aataataaaa gctggttgtg attgtgttat catttccttt gaatcaaagt
    16381 ttttagctgt tattgtacca tctactggag atgttaatgt taatttgtct aagttactca
    16441 ttgctaaatt atatgatact tgagcttggt caagctgttt tttagcaagt gccttagctt
    16501 gaggtatact ttttgaagtt gctgtatcgt atgctctttg agcagaatct aactgttgtt
    16561 ttgtttgctc taaagaaaac tctatttttt taaattggtc taaactaatg acttcctgtt
    16621 cataaagaat cttattattg tcataatttc tttgagactc ctcatattga atctttgcat
    16681 tgtcaagagc cgtttttgcc tgatttactt gtgcctctaa tgagccacca gtggcgcttg
    16741 aataacttgc ttttgctaaa tctagtgagg ctttactttg ctttacacta ttttctagct
    16801 ctggactgct tatactcaat aattcatctc cttttcttac attttgccca agttttacat
    16861 acacttcttg aactgtgcca acagtctgtg cagtaacagc tgtttcatct ttaacctttg
    16921 tagttcctga gaaagaattt gtgttttcaa tttctccacc tactgctttt tgaacagaaa
    16981 ctgctattgg tttctcaggt tcagattctt ctgtttttcc acatccaact aaaaacagtg
    17041 aactacacat taagaaaact attaaatatc ttttcatatc tcctcctatt atatattgca
    17101 cactagcttg ttgtagaatt taaagtgtct tataatttaa gtttagtttt agactaatta
    17161 aaaaacttcc ctttattggc aatttataat tctctaattt gattataaat ttataattat
    17221 tatgagtcaa taactttttc ttgttttaaa tttattaata gtttataatt tatactatat
    17281 taaacaacaa tgttttatta tattatctat tgaagggatg ttacaaatat ggataacaac
    17341 aatatactta aaatagcgga ctctttttta gattttttat ttgtgcttga aaataatata
    17401 tttaaagaaa gcgatttgtt aaaaaagttt caaaataact ctgatataat gaaagcttat
    17461 tttgaagaat gtccaatggc tccatcgcat gctaaggtaa ttctttatct tatgacatca
    17521 aattcatcat caatttctca aatagcttct aatttaggaa ttttaaaatc aaatatgact
    17581 ccgattattg acagattagt ggaacatggt ttagtcaata aatttcctga cccaaaagat
    17641 agaaggattc ttagggttga attaacggat aaagcatttg aattatttga tgcagtaaaa
    17701 gctattctca aagaatctct agtaaaaaaa ttatctaatc tttctgaaga ggaactaact
    17761 ttattagatg aacacacatt aaagttatca gaaatagtaa aaaaattggg ttagaatgtg
    17821 attgtaaaag tcactattaa aaaagttaaa acttcataag aaaagaaata gcagtagtat
    17881 aatatagatt attatactac tgctatttct tttcttatga atatttagtt gtcaaactct
    17941 attttccaat tgaaatagcc agtatctggg tttttattat actttaataa agcatcaaat
    18001 ttattaccat ttttagcggt taaagatttt actttagttt cacctttttt taaaatattt
    18061 ttgaccattg ttttgttaac tttcttttta taaagactta aatacttatc atttttccaa
    18121 ataacaaatt tacattcttt atgattttta caagcaaagc ctttgtcaaa ttccagtaca
    18181 tcaccttgac aaacggggca tttgccaaga gatggatttt catcatcatt tttaacttct
    18241 tttttgttat tagaatcttt tgatatagca acttcttttt cagcattagt tacatctttt
    18301 ttctgctttt tttcttttgt tttagtaaat tttttagttt ttgaatcgaa agtatatgct
    18361 tctgtgttta tattttttga agcatcttgt tttataatat tgacattttg atatataaaa
    18421 ctaatgatat tagtaagata ttcttttctt gtatactttc ctttttgaat atcgcttaaa
    18481 cttttttcaa gtttcccagt ataatctaca tcaaataagt ctttaacagg aaaaatttca
    18541 actaaatttt tcccaaggtc tgttatagaa taagatttcc cttttttctt tacatagcct
    18601 acttgactta tcttctttaa cacatcagca cgtgtggctg atgttccaat agaatatcca
    18661 gacagcactg ttgtatcttc ttcaggaaca tttttaccac aatttttcat agcctttaaa
    18721 agggtgtcct cagtatacgg tttaggtgga gtagtttgct tagttaatgg ttttatttct
    18781 aacacatcta caacatcatc tttatttaca agtggaagta aatcgttttt ttcttcctta
    18841 ttatatacct caagatatcc ttttgattta agcaccttac cttttgttaa gaaagtacaa
    18901 ttatctactt cagtcttaat ttctgtattc tcatattcag caggaggcat aaagtttgct
    18961 acaaatctgt cttttatggc atcataaact aattgttcat ccttagataa actttttgga
    19021 attatataag ttggaattat agcactatgg gagtctactt tagaagaatc aaaaactctt
    19081 ttagttttag aaaatttaat tttattttca tattcaagac caacctttaa tttatctaaa
    19141 gtttgagaag cttttgaagc caaactttct tctaagaaaa tggaatcagt tctagggtaa
    19201 gttatgtatc cacccttacc attaccctca tagagagatt gacagacatt taaaacctta
    19261 tcagatgtaa aattggaata cttagaagtt atatatcctt gaagtgatgt caaactaaaa
    19321 agttttggtg catattcctt tgacatagta acttttttat ctagtatttt accagtttca
    19381 gatgtaattg aagctataat tttatttgca tcttcaagtg tatcaaattt actttcttta
    19441 cccttaacat atttaccttt atattcacca gcttcagctt taaaatgccc ttctatttca
    19501 taataagttt taggaacaaa gtttagtatt tccatatctc tatcatagac taatttaact
    19561 gttggaagaa ttaccctacc tatgtttaat agtttcccat ttccgtattt caacgtagca
    19621 acagaagtga aatttattcc aatcaaccag tcagtaataa gcctagtata tccagcagct
    19681 tgaagatttc tcatttcatc ttcatctttt aggtttttta tacctcttgt aatatcttct
    19741 ggcgtccatt catttaccaa gattcttttt actggttttt tattttttgc cagtaaaaat
    19801 attaaaaatg ctataagttc tccttctcgg tcattgtcag tggcatttat tacatattct
    19861 acgtcttttc tattcaaaag cttttttaca gtgttgaatt gtttagtttt ggaactatca
    19921 atcttaaatt taaacttatc ctcaggaaca aatgggaaat tgttcatttt ccaagagcca
    19981 gagtatttat ctttatcata atccttcata tcatataatg atactaagtg tccaactgca
    20041 taagtaatta tataatcatt tccttcaaag taaccatctt gtctggtctt tgcacctaaa
    20101 aatgatgcta tagtttttgc aactgatggt ttctcagcaa gtacaagttt cataaaaaat
    20161 cctcctttaa aacactaata attatattac cacaaaaaaa taaaaaactt caagaggtcg
    20221 aaatttaagt tataatattt atagaaagtt gaaaggagat ataagatgta caatttaaaa
    20281 gtaaaaaaat taaatgatga tgctataata cctaattttg ctcacaaagg tgatgctggt
    20341 atggatttgt attctataga agaggttgta ataccttctg gagagactaa acttatcaaa
    20401 actgggatat gtatagagct tcctactatg acagaagcac aggtaagacc tagaagtggg
    20461 cttgccttaa agcattctgt tactgtatta aatacaccag gaactattga cgaaggatat
    20521 agaggagagc ttaaaattat attaataaat catgggaaaa atgattttaa ggttgaaaag
    20581 catatgaaaa ttgctcaaat gatagtaaaa cctatctatg atattagcat agaggaagta
    20641 aaagaattaa gtgactccga aagaggaaaa ggtgggtttg gctcaactgg attttaaaaa
    20701 gttacaaaaa agttaaaaaa tgtagccatt tttaaaaaag gtaaaagatg gctatattac
    20761 taatttacag gctattaaag attaaaattt atacatggta atttatggta agcaaatatt
    20821 actatagaca aatagttaca aatttgtaat aatattaata taaaagaaat aattgatatt
    20881 ttaatagtgt caattatttc ttttatattt tgaagtgaaa taaagatagt agaaaagagg
    20941 agatgagtgg aaagttatga attttaatca aaagagaata gcagccagta taatggcaac
    21001 tgcgataata atgcctacta tggggaattt agcatatgct aatgagtctg aagtagagag
    21061 tgtgagtata gaaagcagaa caataacagg gaatgcggta aattttagaa aaggaccagg
    21121 tacaaaccat gaatcaatgg ggaaacttta caaaggtgat aaagttgagt atgtaggaaa
    21181 agaaggttct tgggtaaaag ttaaatataa tggaaacaca ggttatgtgc atggaaatta
    21241 tgtagcaata aatagccttg gaagtagcaa tgaaagtagt gatacttcag ttaagtcgac
    21301 aaaagttgta acagcaaaag gattaaattt tagaacagga ccaagtacaa gtagttctaa
    21361 aatatcaaca ctaggatatg ggactgaagt cggatacata tctgagtcaa atggatggtc
    21421 aaaaataagt tctaatggta gagtgggtta tgtttcaagt aaatacttag gaactagtgt
    21481 aaatgactct acaaatgaaa atgctgaaaa tagttcaaat gaccttgtaa agggtacaaa
    21541 ggttgtaaca gcaaagtcat tgaacgttag aacagggcca ggtacaagcc attctaaaat
    21601 agctacatta agttatggta ctgaggttgg aagtatttct gaaagtggag gatggacaaa
    21661 agtaagttat ggcaatcaaa ctggatatgt gtcaagtcaa tatcttgctg aaaaaggttc
    21721 tgttgatact tcaattccat cttattcgac taattctcca agtcaaggag ctgaaagtgt
    21781 aataagtttt gcaaagacac tacttggaaa accatatgta tggggagcag aaggtccaaa
    21841 tagttttgac tgttctggat tcacacagta cgttatgaaa aaatctgcag gagttagtat
    21901 accaagagta tctagagacc aaagtaagta tggaacatat gtaaatagag gagaccttag
    21961 aagtggggac ttagtattct ttgatacaca aggttcaaat aatggttctg taagtcatgt
    22021 tggaatatac ataggaaatg gagatatgat acatgcatct tcaggttcag caaaaaaagt
    22081 aacaatatct aacataaata gtagctacta ttcaagtaga tatgtaaacg caagaagagt
    22141 tttataatta tttttattat aaaacataat aaatagattt ttctttattg cttataatat
    22201 gaaagcaggt acaatattga tagtaatgta tagtataata catgttacta tttgattggt
    22261 acctgctttt tatttatttt ctct
//
LOCUS       DAEPXF010000055        22278 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain HB27
            SAMN13376389-rid12479423.denovo.059, whole genome shotgun sequence.
ACCESSION   DAEPXF010000055 DAEPXF010000000
VERSION     DAEPXF010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13376389
            Sequence Read Archive: SRR10518432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 22278)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22278)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001069624.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001069624.1
            Genome Coverage       :: 315x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/15/2021 01:54:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,165
            CDSs (total)                      :: 4,076
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 89
            rRNAs                             :: 10, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 10 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 19 of 69
            Pseudo Genes (incomplete)         :: 44 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 10 of 69
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22278
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13376389-rid12479423.denovo.059"
                     /strain="HB27"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="China: Hebei"
                     /collection_date="2017"
     gene            complement(174..1190)
                     /locus_tag="KRL67_001774"
     CDS             complement(174..1190)
                     /locus_tag="KRL67_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HBH1522654.1"
                     /translation="MNYNDLLKSARENFNGTCKVCKICNGLACAGNVPGMGGKGSGSS
                     FIENRKSLEKIKINMRVIHNVSKPDTSIELFGRKMSSPIFAAPVSGTLLNMGGKVSEK
                     EYIEPVVRGCSNSGIYAMVGDTNVDTFLLDNLDVLKDNSGNGIVFIKPWNNSKIIEKI
                     RLSEEAGAFAVGVDLDACGLINNQFQENPFSPKTIDEIRELVESTRLPFIIKGIMTVD
                     DALMAVESGASAIIVSNHGGRVLDYTPGTCEVLPDIAKSVKGKITILVDGGVRTGVDV
                     VKMLGLGADAVLMGRPFVTASFGGGLDGVEFFIEKVRNELCETMILTGCQNVKDIDGR
                     VIWN"
     gene            complement(1518..1700)
                     /locus_tag="KRL67_001775"
     CDS             complement(1518..1700)
                     /locus_tag="KRL67_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH1522655.1"
                     /translation="MYKDKSDECIHLMTAYIDSISGYYSFIDTQLEDFMVKYGENIVD
                     SNLHSIMMLLCKWGLS"
     gene            2013..2432
                     /locus_tag="KRL67_001776"
     CDS             2013..2432
                     /locus_tag="KRL67_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3785 domain-containing protein"
                     /protein_id="HBH1522656.1"
                     /translation="MSLYKFIYDDKEYLLKEENCSALINDEENPVQGVSISKIIDILN
                     EAEEVDFDVEYYQEACPLCLEGVKEKKKFFPFLEYHFYIFSRDGEYVISNISIDYKGL
                     SFNKLSRANKVDNSYIVSVIICENCQDYIVQIENCIV"
     gene            2563..4404
                     /locus_tag="KRL67_001777"
     CDS             2563..4404
                     /locus_tag="KRL67_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HBH1522657.1"
                     /translation="MEEIDDTILDSMFKLTLNERLLTNNDFIENKIDVVKNLIDCYKT
                     EQNSLMLLKSNFLLALLYGIQGFSEKMKEYILISCKYIDKCLSKDYKFLARFYSQIAI
                     LSLKNEDVNKANLYMDKFNLICDENDFLIEEIIFKSQLIYIKASKCIESSVIIKEIET
                     LYIKIKEINDFNCKKIYFFIIGKIYFLFLNDALIAKVNFLKARKYADLLKDMQVSSLC
                     NIKIGECECSFENYECSMEYFNEVISNKKYRNVNIIQKYRASNNMTKILIKTRNYPVA
                     INNLAKSEVYLEKIKNQGLKEVEKFDLFISLAMYYAKSNEKSFEQSICYLDRAKNILN
                     MIKNIEEYTIYDLEIIYHQAKIYYIFGYYDNALNTNKKLLEKSKNAKNYNYVKLAYKN
                     IYLCFEKIQNYEMSMKYFKMYYELKMMYIKARNRNYIDSLNYRHEYIEKEINNMRKVK
                     LDLDKKKYIDHLTSAYNRTFLNDFLEKQEVSEYCTAFMIDVDYFKEYNDTYGHYNGDI
                     ALKDITIIIKKYLKKDMLMIRYGGEEFLILSVCKDYKKSKIFGRKLCRIVKKFLNNNL
                     TVSIGIDTCKNSSIDIREIIENADKALYKAKESGRNRCLHYHDFKNF"
     gene            4431..6317
                     /locus_tag="KRL67_001778"
     CDS             4431..6317
                     /locus_tag="KRL67_001778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HBH1522658.1"
                     /translation="MKRSLIEDLKRNINKIEYDMLTKFYLNLTDLNYILDYHKDLLNT
                     KLEDNSRVYTLYLISLINYIKKDREASYNYLIESLKYIKKLSLKDDSDIISKIYIYLT
                     LSAISIRKYDKANNFYFSAKKIIRQNHLNELLVLLNMSLCVEISALYQTKTDVIALIE
                     EVSFFKTLKNKALVSRANYIFGRVYLYLFNNFIKSMEYLIECLKLAQENNLYSIEAFC
                     KCIISLCYLESGNNVEAIKYINNVLDVAHNNNSISVTERVSIKIDLIWAYLKEDMNKE
                     AEAILLKTIKLMKVVEGVYKDYLYSFIFLYSAEIELKKDNCSFEKVNTYLILSKNIYK
                     SFGDVFIYNSKLDYIYKLYGDLYIKFGNIDEGISCYIKGLNLVKKSSLNLRKISIFYW
                     LIANGYELKKDFKLAIEYYKSMDWYSDRWEKNNSYKTSQAIHKQYELRQKQESLRLLV
                     DDNKKLENDILKDGLTKLYNRRYLEQQLDLFNGTRDKNMVAILVDIDCFKSYNDNYGH
                     LAGDKVIVKVTDIIRSVFIDITEHIIRYGGEEILILVDSIESMDVEEIDNIELYIKRV
                     FKLLEFEGIEHIYSKIKNYITISAGVSIKRCRSRKDVEMLIEDADKNLYKSKKAGRNQ
                     YIII"
     gene            6325..8325
                     /locus_tag="KRL67_001779"
     CDS             6325..8325
                     /locus_tag="KRL67_001779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBH1522659.1"
                     /translation="MILLRKKIGIIASDIELKERIEELYREDVENGTIIIDILNLDLM
                     ENQGRILVEKGAQAIIGRGGGYSLVIDTVNVPVIPMNMKSTDLLRAIEIAKKYSKKVV
                     LILGDNEVSFDYVGWRNVISTEITEEWFESKYEIRSKVVKYIDQKDEVVIVGGGLACS
                     FARQYGIDSVFATASDESIREAVEYCKKLLDTLGEEKFNNEVLRNILDGIKDGVIAID
                     SNGSIILYNESAKNMLKVERKCALNKYILDVFPKMEWMLDCLHEKEDVEDRKIRNINN
                     LIVNTRTTLIKVDNSTYGVLGIIQDITKLQNLERKIRFDLNQKGLYARYTFDDFLFKD
                     KLTKEFIEEAKKIGKSDYTTLLYGESGSGKEIIAHSIHNISKRKDRPFVAINCATIAE
                     NLLESELFGYEEGAFTGARKGGKRGLFELAHGGTLFLDEINSLSFNIQTKLLRVIEER
                     QIMRIGSDYIIPLDIRIIAATNESLTEKIVMGTFRADLFYRLSSLEINIPPLRDRRED
                     IIPLFNNFVNEVLKDDGLNGINSIDENFVLTKDEMDKLYNYSWPGNVRELKTIAQKYV
                     VTGKIKLRQDRDFKTKKVPPNSEVDKFNSETTASVEVQDESINISKINDGKISIDIKE
                     VNKYVEEKIISMLFAQGLSKNEVAQVLGISRTSLWKKYNKNI"
     gene            8587..9846
                     /locus_tag="KRL67_001780"
     CDS             8587..9846
                     /locus_tag="KRL67_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HBH1522660.1"
                     /translation="MSQSMVGKHAMWPKENDVIFSISGRAQAAEKAFGMDNVINATIG
                     ALMDDSGKLITMKTVYEEYKALDNCEIGAYAALEGQPDYLEAVKKVFFRDYLPEGHIR
                     VLASPGGSGAIKLAVWNYTNEGDEVLTSDWFWSPYVSIAEEANRKVVNYQLFDENRRF
                     NFESFKEKFVNIAEKQGRVFTIINTPAHNPTGYSVADDEWDKILDLSKEVAKDKDKKI
                     IFFVDSAYIDFAGDDDVCRKFFKKFSNLPENVLVLVGFSMSKGFTAYGMRMGAIICIS
                     SSEDIAEEFHYSCVHSCRANWSNCNRSAMAVLSNIVNDPKKFKEYEDEKEIYKNMLTR
                     RADVFVKEAERVGLEILPYIAGFFVSIPCDNPKEVCEELTKHNLFAVPLKMGLRFAVC
                     AVSEDKCKKAPSIIKEALESLEVKINN"
     gene            9907..11694
                     /gene="iorA"
                     /locus_tag="KRL67_001781"
     CDS             9907..11694
                     /gene="iorA"
                     /locus_tag="KRL67_001781"
                     /EC_number="1.2.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate ferredoxin oxidoreductase subunit
                     alpha"
                     /protein_id="HBH1522661.1"
                     /translation="MKQLMTGNEAIARGAYEAGVKYASAYPGTPSTEILENIATYKDA
                     IVAEWAPNEKVALEAAIGGSIAGARTMASMKHVGLNVAADPIFTYAYTGVNGGMVLIT
                     ADEPGMHSSQNEQDNRMYAKFAKIPLFEPSTSQEAKDMIKEAFEVSEKYDTPVLYRVT
                     TRLCHSKGLVECYDREEVEIKEYVKNAKKMVTVPANAQIRRGVVEERMEILKKFSNET
                     DLNYYEINDTKIGVIASGMCCNFAKEVFGKNASYMKLGFTNPLPYEKIKEFAEKVDKI
                     YVIEENDPFIEEQIKAYGIDCIGKDVIPPYGEMTPDVLRKAIFGKTNDTIEYKSELVT
                     PRPPSFCAGCPHRGFFYELGKRKNLIVGGDIGCYTLGFAPPYNGIDYVVCMGSAFGTA
                     HGAQKVLNMKDDNEKRLVGVLGDSTFFHTGINGLLDVVYNRGNSISVILDNRITGMTG
                     HQENPGSGYTLQGAKTKEVDIEGLVKACGIEHVRVINPNNLKEVNEALDWALAIEDEP
                     SVIITRWPCVLKKFSKEDIEEFNNPFKTKCKVDHDKCIGCKLCLKTGCPALSFDKENK
                     LSNIDRNQCVGCGVCAQVCPKQAIVKEEK"
     gene            11696..12271
                     /locus_tag="KRL67_001782"
     CDS             11696..12271
                     /locus_tag="KRL67_001782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate oxidoreductase subunit beta"
                     /protein_id="HBH1522662.1"
                     /translation="MTKSILLVGVGGQGTILASKLLTMGLMEAGYDVKMSEIHGMSQR
                     GGSVSSQVRYGDCVHSPVIEIGGADILVSFEKMEALRWFNYLKPEGKALVNNHRIDSM
                     TVLIGGAEYQESDIDAELSRLNAKVINAADKAEELGNSKIMNVILLGCLVKSMQLESI
                     DWEKIISENVKPKFVELNIKAFHEGMEMVSK"
     gene            12324..13394
                     /gene="buk"
                     /locus_tag="KRL67_001783"
     CDS             12324..13394
                     /gene="buk"
                     /locus_tag="KRL67_001783"
                     /EC_number="2.7.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="butyrate kinase"
                     /protein_id="HBH1522663.1"
                     /translation="MTYRILAINPGSTSTKIAVYDGEEQILVKTIDHPAEEIAKYNTI
                     QDQFEMRKEAVLKILKENSIDLKSLSAIVGRGGVLPPVKSGAYLVNEEMIDVLRHRPV
                     LEHASNLGAVVAHAISEPLGINSYIYDSVAVDELIDVARISGLCGMDRSSAGHALNTR
                     AMALKYAKDKGKDYKSLNLIVAHIGGGVSIYLHEKGRMVDMLSDDEGPFSPERSGRVP
                     ATKLVAACYSGQYSEREMTKKIRGKGGIVSYLNTVDAREVEKMIAEGNEEAKIIYEAM
                     AYQLAKGIGELATVVDGKVDAIIITGGIAYSEMFTSMVKKKVEFIAPVEIMAGENELE
                     SLAFGTLRVLNGEEEARIYSEN"
     gene            13612..14313
                     /locus_tag="KRL67_001784"
     CDS             13612..14313
                     /locus_tag="KRL67_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH1522664.1"
                     /translation="MGKISLKSMIRLFSGFFIFAISSVLMINAHVGLMPWDVLHQGLS
                     IKLGITIGQASIMVGVVIVILDAVFGENIGWGTLLNMTFIGIFIDLVIFSGVIPHANN
                     TYIGVFMVVIGIILAAIASFLYLGVCLGSGPRDGLMIALQKKTNKSVRLVRTILEILA
                     LVVGWLLGGSVGIGTLVSALGLGYVLQIVFRIFKFDTKLLKHRFIIDDIREWKEKKNE
                     EHKCKSSIVIKNEQN"
     gene            14373..14879
                     /locus_tag="KRL67_001785"
     CDS             14373..14879
                     /locus_tag="KRL67_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HBH1522665.1"
                     /translation="MELWDLYNADGIKTGNVIKRGNSIEEGYYHLAVEVWILNSNSQI
                     LIQKRSKSKKTLPNMWGMTTGCIVSGEESLEGAIREAKEEIGIDITKDEMKVFRSMIH
                     EDTLWDVYLVKKEYDISKAILQEEEVSDIKWVSTDEIRQLLKEGLFFEYPEIYELLSE
                     IDNNKLNI"
     gene            15054..16076
                     /locus_tag="KRL67_001786"
     CDS             15054..16076
                     /locus_tag="KRL67_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HBH1522666.1"
                     /translation="MEDNKGLARDSSKYKIVSISVVVIILITFWYMLNMVLLTFIMTF
                     IFYNLLVATRKRIKKFSSLNIPDSLIIIVLYALFAILLVLISYAVVPIIIVQLTELSR
                     VFSDFDVNQFAQSLGPKLYPIVSKLDFNKYISQAGLLIASTATKVGSFGVNILLAFLL
                     SLLLLLEKNEIKNFGDKLSDSKISFIYNSLVFFGKSFVKNFGEVMKVQVMIAFINSVV
                     SMIFLGFMGFPQIWALGFMIFVLGLIPVAGVIVSLIPLTVIAFNTGGITKVFGVLLMI
                     CIVHAVETYILNPKLMSNRTKLPVCFVFIILLVGEHYLGVWGLLIGVPIFMFLMDILG
                     VKFTSR"
     gene            complement(16223..16531)
                     /locus_tag="KRL67_001787"
     CDS             complement(16223..16531)
                     /locus_tag="KRL67_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1540 domain-containing protein"
                     /protein_id="HBH1522667.1"
                     /translation="MKNNNLNCAATNCAYNTSGYCYAGSIKVDGMQATTTENTYCASF
                     EDKYTSGITSRSNETNQVDTDNIHCEAVKCKYNKNELCKAEKVHINERNASCETFEMK
                     "
     gene            complement(16591..17019)
                     /locus_tag="KRL67_001788"
     CDS             complement(16591..17019)
                     /locus_tag="KRL67_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH1522668.1"
                     /translation="MNNKLHELEKNLPETSCSFCTHLSFKGPSLDYRYDIRCVISDTK
                     PDFRGCCEYFEPEYTELNTGDLDNLYINFLETCLKVNYNDYINSMYWKLFKERTLLEN
                     NFKCSICGSTENVDVYHVNKNLGRETSKDVVVKCDKCNCK"
     gene            complement(17242..18699)
                     /locus_tag="KRL67_001789"
     CDS             complement(17242..18699)
                     /locus_tag="KRL67_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation protein A"
                     /protein_id="HBH1522669.1"
                     /translation="MTTFLIGLAILLIGGALYGAYCEKVFGPDDRKTPALAQSDGVDY
                     VPMKKWKNSLIELLNIAGTGPILGPIQGILFGPIAFILIPIGCVFGGALHDYMSGMIS
                     IREKGAQMPSLISRFLGNKVFQVYNIFLCLLMLLVGAVFIYTPGDLVVTQILNMKSTI
                     NNPVVWIVYGLIFLYYLCATLFPIDKIIGKIYPIFGAILLLSAAGVGIGIFTQGYDLA
                     NLSLANWKGIHPDGIPLIPTFFVTVACGIVSGFHSTQATLIARSVSNEKEGKTTFYNM
                     MILEGLIAMIWAAAAMGIYNKGIPKELVGSPDVIGLVARDLLGSIGGIIAIIGVIVLP
                     ITSGDTALRSLRLMLADYFHYDQKEKKHRVILSICIFIPVIAILIFAKLSASGFNILW
                     RYFSWSNQTIAIFAFAMITVYLIIKEKNYIISLIPGMFYSFVIFSYIFNAQIGFNLNI
                     NISYVLAAIFTVLYAILTVRSGRKLKSKADTKLAD"
     gene            19117..20031
                     /gene="nadA"
                     /locus_tag="KRL67_001790"
     CDS             19117..20031
                     /gene="nadA"
                     /locus_tag="KRL67_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HBH1522670.1"
                     /translation="MDKDLTYQIKELKKEKNAIILAHFYQPPEIQELADAVGDSYYLS
                     EIARDCKEEVVVFCGVRFMGESAKILSPEKTVLMPVSNAGCAMADMVDEEGVIKLKQQ
                     YPNALVVCYINSTAKVKAHCDVSVTSSSAIKILENIDNKEIIFLPDKNLGGYIAEQFP
                     DKSFIFWDGYCKYHNNIRAEEIIELKDKYKNAEVLVHPECKKEIRDLGDYVGSTSGII
                     KYATNSKNKDFIIATEEGILHELKKNNPNKNFYIPGGKILCTDMKKTTLENLYSTLKN
                     MENEVIVEDEIMEKALNSLLNMHKLAEG"
     gene            20044..21345
                     /locus_tag="KRL67_001791"
     CDS             20044..21345
                     /locus_tag="KRL67_001791"
                     /EC_number="1.4.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
                     /protein_id="HBH1522671.1"
                     /translation="MNLEQDVLIVGSGVSGLYCALNLDKSLNVLVVSKSTIENNNTYL
                     AQGGISTARNIDDIESFVEDTMKAGQYKNRVEAVQVLADESIENVGQIVEYGMPLDKE
                     NGEIDYTREGAHSVNRIVHSKDNTGEVVFKTLLKEAKTRENITLIEDAYLLDILKDGN
                     KCIGARIFKSKKEIHVFSKIVVLATGGIGGLFKNSTNQRHLTGDGIAIALRNNIKIEN
                     LEYIQIHPTAFYEENNEGRRMLISESLRGEGAKLLNKNKERFVDELLPRDVVSKAIFE
                     QMEKDKLPYVYLDATHLDSEYLINRFSFIYNECLARGTDITKECIKVSPAQHYFMGGI
                     HVDLDSKTSMENLYAVGEISCTGVHGANRLASNSLLEGLVFSKRAAKNINSVIDNVKV
                     KFIDVPDMDIDIEQVKKENKILVIKEIERTSEDFGDELFDY"
     gene            21326..22162
                     /gene="nadC"
                     /locus_tag="KRL67_001792"
     CDS             21326..22162
                     /gene="nadC"
                     /locus_tag="KRL67_001792"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HBH1522672.1"
                     /translation="MNYLIIDRMIQDALIEDVPSEDITTNSIVDESSKSTVDLICKQD
                     GIIAGLGVFKRVFEILGDVDVKLYKNDGDRVKNREKIAVLTGSTRNLLVGERVALNYL
                     QRMSGIATITSRYVEKLEGTNTKLLDSRKTIPNLRILDKYSVKVGGGCNHRFNLSDGI
                     LLKDNHMGAAGGVKNAVELARKNTSFVRKIEVEVETLDMVKEAIEANADIIMLDNMSL
                     EMAKQAVDIINGRAIVEFSGNVNLDTIEEIGKIGVDVVSVGALTHSVKALDISMKNLR
                     NI"
BASE COUNT         8765 a         2412 c         3816 g         7285 t
ORIGIN      
        1 tattatatta ttaaataaat ttagctaata ttttgttttt gttaaatata aaaagtgtct
       61 accatagttt gtatagtaat atttgagatt ttaattatca ctattttttg taagaataca
      121 caatttatat ttaaatacct gacagaatat ttaaatataa atttgtttta aaactaattc
      181 catataactc taccatctat atccttaaca ttttgacaac cagtcaaaat catagtctca
      241 cataactcat tcctaacctt ctctataaaa aactccacac catcaagacc accgccaaaa
      301 gaagcagtaa caaaaggtct tcccatcaaa acagcatcag cacccaatcc aagcatctta
      361 actacatcaa caccagtcct aactccacca tcaacaagta ttgttatctt tccctttaca
      421 gacttagcta tatctggaag tacctcacaa gtaccaggag tataatcaag tactcttcca
      481 ccatgatttg aaacaatgat ggcactagca ccagactcta cagccatcaa agcatcatct
      541 acagtcataa ttccttttat aataaaaggt aatctagtag attcaaccaa ttctctaatc
      601 tcatcaattg tcttaggaga aaaaggattt tcttgaaatt gattattaat aagtccacaa
      661 gcatcaaggt ctacaccaac tgcaaaagca cctgcttctt cagacaatct aattttttct
      721 attatctttg aattattcca aggctttata aatacaattc catttccact attatctttc
      781 aacacatcta aattatctag taaaaaagta tctacattag tatcgccaac catagcataa
      841 attccagaat tagaacaacc tctaacaaca ggctctatgt attctttttc agaaacttta
      901 ccacccatgt tcaatagagt acctgataca ggtgcagcga atataggaga actcattttt
      961 ctaccaaata attctatgga tgtatctggc tttgaaacat tatgtatgac tctcatattt
     1021 atctttattt tttctagact ctttctattt tcaatgaaag acgaaccact tcctttacca
     1081 cccataccag gaacatttcc agcacaagct aatccattac aaattttaca tactttacat
     1141 gttccattaa agttctctct agctgatttt agtaagtcat tgtaattcat acaaaatctc
     1201 tccttttata atattaaatt tgagtattac taaattgaaa atatcttaat tgtaaatata
     1261 gagatgatgt tatgatgagc taaactagat aatacatatt agatattttt tattgttgta
     1321 gatagttaaa ttatatcata ttagatagtc aaattttgaa aaaaactaac ttatttaagt
     1381 atacattttg aaaaaattaa aaaagctata tatagatttt taaagaaatc tatatatagc
     1441 ttttaagttt atgtcaatct tttttattag tgaataaata atatagaata caatatacaa
     1501 aaaagtttct tgaaatgcta agataatccc catttacaaa gcaacatcat tatactatgt
     1561 aaatttgaat caacaatgtt ttcaccatat ttcaccataa aatcctctaa ttgtgtgtca
     1621 ataaatgaat aatatccact tatactatcg atgtatgcag tcattaagtg tatacattca
     1681 tcacttttat ctttatacat atatatctcc ccctatatac aatatataaa tttagatacc
     1741 aaatcgtatc atattaaata gttaagtgtg taacatctat gcagttatat atttatatcc
     1801 ttatattaat aactaaagtg agttttgaca atagattttg ggaaactgga tgttcaaagt
     1861 gcaaacttgg attttaaaat cattaattaa gatagtgcat aagaatatat gtatacataa
     1921 cttaattata aagattataa acaattactt ttattaactt tatttaagtg gtagaataac
     1981 ttggagatta ataattataa ggaggatata atatgagttt atataagttt atatatgatg
     2041 ataaagaata tttattaaaa gaggagaact gtagtgcttt aataaatgat gaagaaaatc
     2101 cagttcaagg agtaagtatt tcaaaaataa ttgatatact taatgaagca gaagaagttg
     2161 attttgatgt agaatattat caagaagcat gtccattatg tttagaagga gtaaaagaaa
     2221 agaaaaaatt ctttcctttt ttagaatatc acttttatat tttcagtaga gatggagaat
     2281 atgtaataag caatatatct attgattata aaggactatc ttttaataaa ctatcaaggg
     2341 caaataaagt tgataatagt tatattgtaa gtgtgataat ttgcgaaaat tgtcaggatt
     2401 atatagttca gatagaaaat tgtatagttt agtgtaaagt atgtcttaat ttaagagaaa
     2461 tttctaaatt gagacatcaa cttttttcaa aaatagtatg gaaaattact aaatttatga
     2521 gataatgtaa gtcggaggtg agttacaata tatagtaaat atttggaaga gatagatgat
     2581 acaattttag attcaatgtt taaactaacc ttgaatgaaa gattattgac taataacgat
     2641 tttattgaaa ataaaattga tgtagtaaag aatttaattg attgctataa aactgaacaa
     2701 aatagtttaa tgttattaaa atcaaatttt ttgctagctt tattatatgg tattcaaggg
     2761 tttagtgaaa aaatgaaaga gtatattttg atttcctgta aatatattga taaatgctta
     2821 tccaaagatt ataagttttt agcacgattt tattcacaaa tagctatttt aagtttaaaa
     2881 aatgaagatg taaataaggc aaatttatat atggataaat ttaatcttat ttgtgatgaa
     2941 aatgattttc taattgaaga aattattttt aaatcacaac ttatttacat taaggcaagt
     3001 aagtgtatag aatcttctgt aataattaaa gaaatagaaa cgttgtatat aaagataaaa
     3061 gaaattaacg attttaactg taaaaaaata tattttttta ttattggtaa aatatatttt
     3121 ttgttcttga atgatgctct aattgctaag gtgaattttt taaaagcaag aaaatatgca
     3181 gatttactaa aggatatgca agtatcttca ctatgtaata taaagatagg tgagtgtgaa
     3241 tgttcgtttg aaaattatga atgctctatg gaatatttca atgaagtaat aagcaataaa
     3301 aagtatagaa atgtaaacat aattcaaaaa tatagagcat caaacaacat gactaaaata
     3361 ttaataaaga ctagaaatta tcctgtcgca ataaataatt tagcaaaatc tgaagtatat
     3421 ctagaaaaaa ttaaaaatca gggcttaaaa gaagtagaga agtttgattt atttataagt
     3481 ttagccatgt attatgcaaa aagtaatgaa aaatcttttg aacaatcaat atgctattta
     3541 gatagagcta aaaacatctt aaatatgata aaaaatatag aagaatatac aatatatgat
     3601 ttagaaataa tttatcacca agcaaagata tattatatct ttggatatta tgacaatgcc
     3661 ttaaacacaa ataaaaaact ccttgaaaaa tctaaaaatg ctaaaaatta taattacgtg
     3721 aagttagcat acaagaatat atatttgtgt tttgaaaaga ttcaaaatta tgaaatgagt
     3781 atgaaatact ttaaaatgta ttatgagtta aaaatgatgt atataaaagc tcgaaataga
     3841 aattatatag attccttaaa ttatagacat gagtatatag aaaaagaaat aaataacatg
     3901 agaaaagtaa aacttgattt agataaaaaa aagtatatcg accatttgac aagtgcatat
     3961 aataggactt ttttgaacga ttttttagaa aaacaagaag ttagtgaata ttgcacagca
     4021 tttatgatag atgtagatta ctttaaagaa tataatgata cttatggaca ctacaatgga
     4081 gatattgctc ttaaagatat aactataata ataaaaaaat atctaaaaaa agacatgctt
     4141 atgataaggt atggtggaga agaatttctg atattatctg tatgtaaaga ttataaaaaa
     4201 agtaaaatat ttggaagaaa attatgtagg attgtaaaga aatttttaaa caataatttg
     4261 acagttagta ttggcattga tacatgtaaa aatagtagta tagatataag agaaataatt
     4321 gaaaatgctg ataaagcttt atataaagca aaggaaagtg gtagaaatag atgtttgcat
     4381 tatcatgatt ttaagaattt ttaactgttg aaataggatt ggtgatttac ttgaaaagga
     4441 gtttaataga agatttaaag agaaatataa acaagataga atacgatatg ttgactaaat
     4501 tctatctaaa tttgacagat ttaaactaca tcttggatta tcacaaagac cttttaaata
     4561 caaaactaga agataatagt agagtctata ctttatattt aattagtctt ataaactata
     4621 taaaaaaaga tagagaagca tcatacaact atttgataga gtcattaaag tatattaaaa
     4681 aattgtcatt aaaagatgat tcagatataa tatctaaaat ttatatttat cttactttat
     4741 cagctatttc tataagaaaa tatgataagg caaataattt ttatttctca gcaaaaaaaa
     4801 ttataagaca aaatcattta aatgagttgt tggtcttgtt aaatatgagt ttatgtgtag
     4861 aaataagtgc tttatatcag accaaaacag atgtaatagc tttaatagaa gaagtgtctt
     4921 tttttaaaac tttaaaaaat aaagctcttg taagtagggc aaattatata tttgggagag
     4981 tttatctata tttatttaat aactttataa aaagtatgga gtacctaatt gaatgtctaa
     5041 aactggctca agaaaataat ctatactcta tagaggcttt ttgtaaatgt ataataagtt
     5101 tgtgttattt agagagtgga aataatgtag aagctataaa atacataaat aatgtattag
     5161 atgttgcaca caataataac tcaatatcag taactgaaag agtgtccata aagatagacc
     5221 ttatatgggc atatttaaaa gaagatatga ataaagaagc cgaagctatt ttattaaaga
     5281 ctataaaatt aatgaaagtt gttgaaggtg tatataagga ttatttatat tcatttatat
     5341 ttttatattc agctgaaatt gagttaaaga aagataattg tagttttgaa aaggtaaaca
     5401 cctatttaat tttatcaaaa aatatatata aaagttttgg agatgttttt atatataatt
     5461 ctaaactaga ttacatatat aagttgtatg gagatttata tataaaattt gggaatatag
     5521 acgaaggtat aagttgttat ataaaaggtt taaatttagt taaaaagagt tcattgaatc
     5581 ttagaaaaat atctatattt tattggctaa tagcaaatgg atatgagcta aaaaaagatt
     5641 ttaaactggc aatagaatat tataaaagta tggattggta ttctgatagg tgggaaaaaa
     5701 ataattccta caaaacatct caagcaattc acaaacaata tgaacttaga caaaaacaag
     5761 agagtttaag attactagtg gatgataata agaaattaga aaatgatatt ttaaaagatg
     5821 gacttactaa actatataat agaagatatt tggaacagca gttggattta tttaatggaa
     5881 ccagagacaa aaatatggtc gcaatactgg tcgatataga ttgttttaaa agttataatg
     5941 ataattatgg tcatctagca ggagataaag ttatagttaa ggtaactgat attataagaa
     6001 gtgtatttat agatattacc gaacacataa tcagatatgg gggagaagaa attttaatat
     6061 tagtagattc tatagaatca atggatgttg aagagattga taatatagaa ttatatatta
     6121 aaagggtttt caaacttctt gaatttgagg gtattgaaca tatatattct aaaataaaaa
     6181 actatataac tataagtgct ggagtaagta taaaaagatg tcgtagtaga aaagatgttg
     6241 agatgttaat tgaagatgca gataagaacc tatataaatc aaaaaaagca ggtagaaatc
     6301 aatacattat aatttaaaat tgaggtgatt ttgttgcgaa aaaaaatagg aattatagcc
     6361 tctgatattg aattaaaaga aagaatagaa gaattatata gagaagatgt agaaaatgga
     6421 actataataa tcgacatact taacctagac ttaatggaaa atcaaggtcg tatacttgtt
     6481 gaaaaaggtg cccaagctat aattggtaga ggtggaggtt atagtttagt aatagataca
     6541 gtaaatgtac ctgttatacc aatgaatatg aagtcaactg accttttaag agcaatagaa
     6601 atagcaaaga aatattctaa aaaagtagta ttgattcttg gagacaatga agtttctttt
     6661 gactatgttg gctggagaaa tgtcataagt actgaaataa ctgaagagtg gtttgaatct
     6721 aagtatgaga taagaagtaa agttgtaaaa tatatagacc aaaaagatga agttgtaatc
     6781 gtaggtggag gtttggcatg ttcttttgca aggcaatatg gaattgatag tgtatttgca
     6841 actgctagtg atgaatctat aagagaagct gtagaatatt gtaaaaaatt attggataca
     6901 ttaggtgaag aaaagtttaa taatgaagtg ttacgaaata tacttgatgg tataaaagat
     6961 ggagtaattg ctatagatag taatggtagt ataattttgt acaatgaaag tgcaaaaaac
     7021 atgcttaaag tagagcgaaa atgtgcactt aataaataca tcttagatgt atttcctaaa
     7081 atggagtgga tgttagattg tttacatgaa aaagaggacg tagaagatag aaagataaga
     7141 aatataaata atctaatcgt taatactagg acaacattaa taaaagttga taatagtact
     7201 tatggagttt taggaataat acaagatata accaagctac aaaacttaga aagaaaaata
     7261 agatttgact tgaatcaaaa aggtctctat gctagatata cttttgatga ttttttattt
     7321 aaagataaat taactaagga atttattgaa gaagctaaaa aaattggaaa aagtgattat
     7381 acaactttac tttatggaga aagtgggtct ggtaaagaaa ttatagctca tagtatacac
     7441 aacataagta aaagaaaaga tagacctttt gttgccataa attgtgctac tatagcagaa
     7501 aatttacttg aaagtgaact ttttggatat gaagaaggtg catttactgg tgctagaaaa
     7561 ggtggaaaaa gaggactatt tgaacttgct catggaggga ctcttttttt agatgaaata
     7621 aatagtctat cttttaatat tcagacaaaa ctattaagag taatagagga acgtcaaata
     7681 atgagaattg gttctgatta tataatacct ctggatataa ggattatagc agcaacgaac
     7741 gaatctttaa ccgaaaaaat agttatgggt acatttaggg cagacttatt ttatagattg
     7801 agtagtttag agataaatat acctccttta agagacagaa gagaagatat aatacccttg
     7861 tttaacaatt ttgtaaatga agtattaaag gatgatggtc tcaatggaat aaacagtata
     7921 gatgaaaatt ttgttttgac taaagatgaa atggataagc tatataatta tagctggcct
     7981 ggaaatgtaa gagagttaaa gacaatagct caaaaatatg ttgtgacagg taaaattaaa
     8041 ttgagacaag atagagattt taaaactaaa aaagtacctc ctaactctga agtagacaaa
     8101 tttaattctg aaacgacagc aagtgtagaa gtccaagatg aatctataaa tataagcaag
     8161 ataaatgatg gtaagataag tatagatata aaagaagtaa ataagtatgt tgaagagaaa
     8221 ataataagta tgctttttgc tcagggactt tctaaaaatg aagttgcaca agttcttgga
     8281 ataagtagga catctttatg gaagaaatat aataaaaata tttaaatata attacatata
     8341 aattattttg aagtaatgtt aattttttta aacaagtgtt aagaaaaatt aacattactt
     8401 ttttgcaatt ttttattttt ttagacaggc atatatattt ataagaaaaa tagttaaaac
     8461 acttaattta ataatgaaaa aaataataat tctaaacaaa atcagtttta cataaagtgg
     8521 aatttattgg catagtaatt gcttaatata taatgcaaga gtaaatacaa ttttaggggt
     8581 gatggtatga gtcaaagtat ggttggtaag catgcaatgt ggccaaaaga aaatgatgtt
     8641 atatttagca tatctggcag agctcaagca gcagagaagg cttttggtat ggataatgta
     8701 ataaatgcaa caataggcgc tctaatggat gattcaggaa aattaataac aatgaaaact
     8761 gtatatgaag aatataaagc attagataac tgtgagatag gtgcatatgc agctcttgaa
     8821 ggtcaaccag attacttaga agcagttaaa aaagtatttt tcagagatta cttaccagaa
     8881 ggacatataa gagtattagc ttcaccagga ggaagtggtg caataaaatt agctgtatgg
     8941 aactatacta atgaaggaga tgaggtatta acatctgatt ggttctggag tccatatgta
     9001 agtatagctg aagaagctaa tagaaaagta gttaattacc aactttttga tgaaaataga
     9061 aggtttaatt ttgagtcgtt taaagaaaaa tttgtaaata tagcagaaaa acaaggtaga
     9121 gtctttacaa ttataaacac accagcacat aatccgactg gatatagtgt tgcagatgat
     9181 gagtgggata aaatattaga cttatcaaaa gaagtcgcaa aagataaaga caaaaagata
     9241 atatttttcg tagattcagc atatattgac tttgcaggtg atgatgatgt atgtagaaag
     9301 ttttttaaga aattttctaa tttgcctgaa aacgttctcg tattggttgg attcagtatg
     9361 tcaaaaggat ttacagctta tggtatgaga atgggggcta taatttgtat atcttcaagt
     9421 gaagatatag ctgaagagtt ccattactcc tgtgttcatt catgtagagc aaattggtca
     9481 aattgtaata gaagtgccat ggcagtatta tctaacattg taaatgaccc taaaaaattc
     9541 aaagaatatg aggatgaaaa agaaatatac aaaaacatgc ttactagaag agcagatgta
     9601 tttgtaaaag aagctgaaag agtaggatta gaaattcttc catatatagc aggtttcttt
     9661 gtaagtatac catgtgataa tccaaaagaa gtatgtgaag aattgactaa acataattta
     9721 tttgcagttc cattaaaaat gggcttaaga tttgcagttt gtgcagtttc tgaagataaa
     9781 tgtaagaaag caccaagtat tataaaagaa gcacttgaaa gtttagaagt aaaaataaat
     9841 aattaatttc attgaacaaa ttgggtggac tatgaattta ataattgtag atggagggaa
     9901 ttttatatga aacaattgat gacaggtaat gaagcaattg cacgtggagc ctatgaggct
     9961 ggtgttaaat atgcatctgc atatccaggg actccaagta cagaaatatt agaaaatata
    10021 gcaacatata aagatgcaat agttgcagaa tgggctccaa acgaaaaggt agcacttgag
    10081 gctgctatag ggggttcaat tgcaggagca agaacaatgg cttctatgaa acatgttggt
    10141 ttgaatgttg cagctgatcc aatatttact tatgcatata caggggtaaa tggtggtatg
    10201 gtacttatta cagcagatga gccgggaatg cattcatctc aaaatgagca agacaatagg
    10261 atgtatgcaa aatttgcaaa gataccttta tttgaaccat caactagtca agaagctaaa
    10321 gatatgataa aagaagcttt tgaagtcagt gaaaaatacg atacaccagt tttatataga
    10381 gttacaacaa gactttgtca ttcaaaagga ttagtggagt gctatgatag agaagaagtt
    10441 gaaattaaag agtatgttaa aaatgctaag aaaatggtaa ctgttccagc aaatgctcaa
    10501 ataagaaggg gcgttgttga agaaagaatg gaaattctta aaaaattctc aaatgaaact
    10561 gatttaaact actatgaaat aaatgataca aaaataggtg ttatagcttc tggtatgtgt
    10621 tgtaatttcg ccaaagaagt ttttggaaaa aatgcatcat atatgaaact aggatttaca
    10681 aatccattac cttatgaaaa aataaaagaa tttgcagaaa aagttgataa aatatatgtt
    10741 atagaagaaa atgacccatt tatagaagaa caaataaaag catatggaat agattgtatt
    10801 ggaaaagatg taataccacc atatggagaa atgacaccag atgttttaag aaaagctata
    10861 tttggaaaaa caaatgatac tatagaatac aaatctgaat tagtaacacc aagaccacca
    10921 agtttctgtg caggatgtcc acatagagga ttcttctatg aacttggaaa gagaaaaaat
    10981 ttaatagttg gtggagatat aggttgttat actcttggat ttgctcctcc atataatgga
    11041 atagattatg ttgtttgtat gggttctgct tttggaactg cacatggagc tcaaaaagtt
    11101 cttaatatga aagatgataa tgaaaaaaga ttggtaggtg tacttggaga ttcaacattc
    11161 ttccatacag gaataaatgg acttttagat gttgtttaca atagaggtaa ttctatttca
    11221 gttatattag ataatagaat aactggaatg acaggtcacc aagaaaaccc aggttcagga
    11281 tatactttac aaggtgctaa aactaaagaa gttgatatag aaggattagt taaagcttgt
    11341 ggaatagaac atgtaagagt tataaaccct aacaatttaa aagaagtaaa tgaagcacta
    11401 gattgggctt tagctataga agatgaacca tcagtaataa taacaagatg gccatgtgta
    11461 cttaaaaaat tctctaaaga agatatagaa gaatttaata atccatttaa gactaagtgt
    11521 aaagttgacc atgataaatg tataggatgt aaattatgtt tgaaaactgg atgtccagct
    11581 ctatcatttg ataaagagaa taagttatca aatatagata gaaatcagtg tgtaggatgt
    11641 ggagtatgtg cacaagtttg tccaaaacaa gcaatagtta aggaggaaaa ataatatgac
    11701 taagagtata cttttggtag gtgtaggagg tcaaggaact atacttgcta gtaaattatt
    11761 aactatggga ttgatggaag ctggatatga tgtaaagatg agtgaaatac atggaatgag
    11821 tcaaagagga ggttcagttt cctcacaagt tagatatgga gattgtgttc attctccagt
    11881 tatagaaatt ggtggagcag atatattagt atcttttgaa aaaatggaag ctttaagatg
    11941 gttcaattat ttaaaaccag aaggaaaagc cttagtaaat aatcatagga tagattctat
    12001 gactgtttta atcggaggag ctgagtatca agaaagtgat atagatgcag agttaagtag
    12061 attaaatgca aaagttataa atgcagctga taaagctgaa gaactaggaa attcaaaaat
    12121 aatgaatgtt attttattag gatgtctagt taaatctatg caattagagt ctattgattg
    12181 ggaaaaaatt ataagtgaaa atgttaaacc aaaatttgtt gaattaaaca taaaagcttt
    12241 ccatgaaggt atggaaatgg ttagcaaata gtttataata gatataaata gactatagaa
    12301 attaaggtga aaggaagaaa attatgactt acagaatatt agccataaat ccaggttcta
    12361 cttctacaaa aatagcagta tatgatggag aagaacaaat tcttgtgaag acgatagacc
    12421 atccggctga agagattgca aaatacaata ctatacaaga ccagtttgaa atgcgtaagg
    12481 aagcagtttt gaagattctt aaagaaaata gtatagactt aaaatctctt agtgcaatag
    12541 taggaagagg tggagtttta ccaccagtaa aatcaggagc atatttagta aatgaagaaa
    12601 tgattgatgt actaagacat agaccagtac ttgaacacgc ttccaattta ggtgctgttg
    12661 tggcacatgc aatatcagaa cctcttggaa taaactcata tatttatgat tctgttgcag
    12721 tagatgagct tatagatgta gcgagaatat ctgggctttg tggaatggat agatcaagtg
    12781 cagggcatgc attaaatact agagcaatgg ctttaaaata tgctaaggat aaaggaaaag
    12841 attataagag cttaaactta atagtagctc acattggtgg cggagtaagt atttatcttc
    12901 atgaaaaagg aagaatggtt gatatgctat ctgatgatga aggaccattt tctccagaaa
    12961 ggtcaggaag agtacctgct acaaaattag tggctgcctg ttattcaggt caatattcag
    13021 aaagagaaat gactaaaaag ataagaggta aaggtggtat agtttcatac ctaaatactg
    13081 tagatgctag agaagttgaa aaaatgatag cagaaggaaa tgaagaagca aaaattattt
    13141 atgaagcaat ggcttatcag ttagcaaaag gtattggaga gttagcaact gtagtagatg
    13201 gaaaggtaga tgctataatt ataacaggtg gaattgcata ttctgaaatg tttacttcaa
    13261 tggttaaaaa gaaagttgag tttatagcac cagtagaaat tatggcagga gaaaatgagt
    13321 tggaatcact tgcttttgga actttaagag tactaaatgg agaagaagaa gctagaattt
    13381 atagtgaaaa ttaataagaa aaactaaaaa tgtttatata ctaaaaaagt ggtatatccg
    13441 ttttttatac acgaaagctt agagtatagg tgaaaatgta taaaatttgg attgccactt
    13501 tttataatta aaattttatc ttaacctaat aaataattct gtaaaatttc gtatttatat
    13561 atatatttta ttggtattta ttgttattat tattatgagg agtgattttt gatgggaaaa
    13621 ataagtttaa aaagtatgat aagattattt tcaggttttt ttatatttgc aatttcttct
    13681 gtgcttatga ttaatgcaca tgtaggtctt atgccttggg atgtacttca ccaaggttta
    13741 tctataaagc taggtataac tataggtcag gcttctataa tggtaggagt tgtaattgtt
    13801 atattggatg cagtttttgg tgaaaatata ggatggggta ctttactcaa catgacattt
    13861 ataggaattt ttattgattt agtaatattt tctggagtga tacctcatgc aaacaacact
    13921 tatataggtg tgtttatggt tgttatagga attattttgg cagctatagc aagttttcta
    13981 tacttaggtg tttgtctagg aagtggaccg agagatgggc ttatgattgc tcttcagaaa
    14041 aaaactaata aatctgtacg tcttgttaga acaattcttg aaatactagc attagttgtt
    14101 gggtggttat taggaggttc tgtaggtatt ggtacgcttg taagtgcttt aggtttagga
    14161 tatgtattac agatagtgtt tagaatattt aaatttgaca caaagttgct gaaacataga
    14221 tttattatag atgatataag agaatggaag gaaaagaaaa acgaggaaca taaatgtaaa
    14281 tcaagtattg taataaaaaa tgagcagaat taattacgtt aaaatatatt tgtagtttat
    14341 ctaatttata gtaaagtcaa ggagattttg aaatggaatt atgggattta tataatgctg
    14401 atggaattaa aacaggaaat gttataaaaa gaggtaattc tattgaagaa ggttattacc
    14461 atttggcagt tgaagtgtgg attttaaata gtaactctca aattttaatt cagaaaaggt
    14521 ctaaaagtaa aaagacattg ccaaatatgt ggggaatgac aactggatgt attgtttctg
    14581 gggaagagag tttagaagga gcaattcgcg aagcaaagga agaaataggg atagatataa
    14641 ctaaagatga gatgaaagtt tttagaagta tgattcatga agatactctt tgggatgtat
    14701 acttagttaa aaaagaatat gatatatcca aagctatttt acaggaggaa gaagtaagtg
    14761 atataaagtg ggtaagcaca gatgaaataa gacaattact aaaagagggt ttgttttttg
    14821 aatatccaga gatatacgaa ttgctatctg agatagataa taataaatta aatatatgat
    14881 gtagagcaga tttcagtata agtaagatta tttaaaatat accttctcta aattttaata
    14941 ttgttttatg aaaattaata agttattttt agtatttttc tacttgtttt ttgagattaa
    15001 aataaaaagg gcagtcatta taaaggcata aaaatttggg gaggattcaa actatggaag
    15061 ataacaaagg attggcacgc gatagttcaa aatataaaat tgtttctata tctgtcgtag
    15121 taattattct aattacattt tggtatatgt taaatatggt gcttttaaca tttattatga
    15181 cttttatttt ctataacttg cttgtggcca caagaaaacg aattaagaag ttttcttctt
    15241 taaatattcc agattcattg ataattatag tattatatgc actatttgct atattgttag
    15301 tattgattag ctatgcagtt gtaccaataa ttatagttca acttacagag ttgagtaggg
    15361 tttttagtga ttttgatgta aatcaatttg ctcaatcttt aggaccaaaa ttgtatccta
    15421 ttgtttcaaa gctggatttt aataaatata tttcacaagc agggctactt atagcatcaa
    15481 cagcaacaaa agttggaagt tttggagtaa atatattatt ggcattttta ctgagtttac
    15541 ttcttttact tgaaaaaaat gaaattaaaa attttggaga taaactttct gatagtaaga
    15601 tttcttttat ttataattcg cttgtatttt ttggaaagag ctttgttaaa aattttggtg
    15661 aagtaatgaa agtacaagtt atgattgcct ttataaattc tgttgtttct atgatttttt
    15721 taggtttcat gggattccct caaatatggg ctttaggatt tatgattttt gtgttgggtc
    15781 ttattccagt agcaggagtt atagtgtctt tgattccatt aactgtaata gcatttaata
    15841 caggaggaat aacaaaggtt tttggagttt tacttatgat ttgtatagtg catgcagtag
    15901 aaacatacat attaaaccct aagttgatgt cgaatagaac gaaacttcca gtttgttttg
    15961 tttttattat tttacttgta ggagaacatt atttaggtgt atggggatta ctaattggag
    16021 ttccaatatt catgttcctt atggatattc tgggtgttaa atttacttct aggtaatata
    16081 ttaaaataat aatttgtttt tatatgtaaa ttatgaattg ttttaaaaaa gaacctataa
    16141 atagacatca ttaataaaga catctatttg ataggttcaa ttttactttt tataaatact
    16201 ataaaattaa ataaaaagaa atttatttca tttcaaaagt ttcacaactt gcatttcttt
    16261 catttatatg aactttttca gctttgcaaa gttcattttt attgtattta cattttacag
    16321 cttcacaatg aatgttatct gtatcaactt ggttagtttc attagaacga ctagttatac
    16381 cagatgtata tttgtcttca aaagaagcac aataagtatt ttctgtagta gttgcttgca
    16441 ttccatctac ttttatacta ccagcataac aatatccact agtattataa gcacaattag
    16501 tagcagcaca atttaaatta ttatttttca taaataacct cctaagtttt atttaaactt
    16561 attatgtgaa gttatctaaa ttttatacac ttatttacaa ttacatttat cacatttaac
    16621 aactacatct ttggaagttt cccttcctaa atttttatta acatgataca catcaacatt
    16681 ttcagttgat ccacatatag aacatttaaa attgttttct agtagggttc tttctttaaa
    16741 taacttccaa tacatagaat tgatgtaatc attataattt accttgaggc atgtctcaag
    16801 aaagtttatg tatagattgt ctaaatctcc tgtatttaac tctgtatatt ctggttcaaa
    16861 atattcacaa catcctctaa aatcaggttt tgtatctgaa ataacacatc taatatcata
    16921 tctatagtct aggctaggcc ccttaaatga aagatgtgtg caaaaagaac aagatgtttc
    16981 tggtagattt ttttcaagtt catgtaattt attattcata agtattcctc caaaaaattt
    17041 attttaagta tataaatgaa tagattattt taaaactttt atttatttat attagagatt
    17101 taggataaat attccaataa ggagtaaata cttaagtatt tattactgta ttaaataaaa
    17161 aagaggcaaa tctaacttga gattgcctac ttttttatca ttttttaata aaacttagta
    17221 taagtattat tttaattaga tttagtcagc caatttagta tctgctttgc tttttaactt
    17281 tctaccagag cgtacagtta aaatagcata taatacagta aatatggcag ctaaaacata
    17341 tgatatattt atgtttaagt taaatccaat ttgagcatta aatatataac tgaatataac
    17401 aaaagaataa aacataccag gaattaagct tataatataa ttcttttctt ttattataag
    17461 gtaaactgta atcattgcaa acgcaaatat ggcaatagtt tgattactcc aagagaagta
    17521 tctccacaat atattaaatc cagaagcact aagctttgca aatataagga tggctatgac
    17581 aggaataaat atacagattg acaatataac acgatgtttc ttttcctttt ggtcataatg
    17641 aaagtaatct gcaagcatta atcttaaaga tcttaaagct gtatctccag aagttattgg
    17701 taaaacaatt acacctatta tagctatgat accaccaata gaaccaagta aatctcttgc
    17761 aactagacct attacatcag gagaacctac taattcctta ggtattcctt tattgtaaat
    17821 acccatagca gctgcagccc aaatcattgc aattaaacct tctaaaatca tcatattata
    17881 aaaagtagtt tttccttctt tttcatttga aactgatctt gctataagag tagcttgagt
    17941 tgagtggaat ccagaaacga taccacaagc aactgtaaca aagaatgtag gaataagagg
    18001 aataccatca ggatgaatac ctttccaatt tgcaagactt aaattagcta aatcataacc
    18061 ttgtgtaaag attccaattc caacaccagc agcagataaa agtagaatag ctccaaatat
    18121 tggatatatc tttcctatga ttttatcaat tggaaatagt gttgcacaca agtaatatag
    18181 aaagataagt ccataaacta tccacacaac tggattattt atagtcgatt tcatatttaa
    18241 aatttgagta acaactaaat ctccaggtgt ataaataaaa actgcaccga ctaaaagcat
    18301 taaaagacaa aggaatatat tatagacctg aaatacttta ttacctaaaa atctactgat
    18361 aagagaaggc atttgtgctc ctttttctct tatactaatc attccactca tatagtcatg
    18421 taaagctcct ccaaatacac agccaatagg tattagtatg aatgctattg gaccaaacaa
    18481 tataccttgg ataggtccta gaattggtcc tgtaccagct atattaagca actcaattag
    18541 actatttttc cattttttca ttgggacata gtctactcca tcactttgag caagtgcagg
    18601 tgtttttctg tcatctgggc caaatacttt ttcacaataa gcaccgtata aagcacctcc
    18661 tattagtaat atagccaagc cgattaagaa cgttgtcata atatcacccc atttataaaa
    18721 ttttttggtc tttgtataca tattatctaa atgtgtagat ttatattgaa tttaattctg
    18781 cacaacctac aaattctata aagtattaca actaatataa tttcagaaat aaatacatat
    18841 acataaccat gtacttagta tacaggtatt tggtaatatt ttcaatgtta tagcaataaa
    18901 gaatataaac aaaattctga taatatttta caaaaaaatt aaaaaatata aaaattagtt
    18961 taaatagatg gggaaaaaag gaaaatcaat tgagaatata aatgtctata tcttaacaat
    19021 attaagaggt ttaaaattct tgaagaaaga atagtcaagt gatataatga atttacaggt
    19081 gtatatacac tagtacatat agaaagcggt gcaattatgg ataaagattt aacatatcaa
    19141 atcaaagagc taaagaaaga aaaaaatgca ataatacttg cccattttta tcaaccacca
    19201 gaaatacaag aattagcaga tgcagttggt gactcatatt atttaagtga gattgctaga
    19261 gattgcaaag aagaagtggt tgtattttgt ggagtaaggt ttatgggaga aagtgctaag
    19321 atattatcac ctgaaaaaac tgtattgatg ccagtaagta acgctgggtg tgctatggca
    19381 gatatggtag atgaagaagg cgtgataaaa ttaaaacaac agtatccaaa tgctttggtt
    19441 gtatgttata taaactctac agctaaagtt aaggcacatt gtgatgtttc tgttacatct
    19501 tctagtgcaa ttaaaatact agaaaacata gataataaag aaattatatt ccttccagat
    19561 aaaaatttag gaggatatat tgcagaacaa tttccagata aaagctttat attttgggac
    19621 ggatattgta aataccataa caatataaga gctgaagaaa taattgagtt aaaagataag
    19681 tataaaaatg cagaggtatt agtacatcca gaatgtaaaa aagaaataag agatttagga
    19741 gattatgtag gtagtacatc aggaataata aaatatgcta ctaatagtaa aaacaaagac
    19801 tttataatag caactgaaga aggtatatta catgaattaa agaaaaataa tcctaataaa
    19861 aacttttata ttcctggtgg aaaaatacta tgtacagata tgaaaaagac aacattagaa
    19921 aatttatata gtacacttaa aaatatggaa aatgaagtta ttgtagaaga tgaaatcatg
    19981 gaaaaggctt taaattcact gcttaatatg cacaaattag cagaaggata gagggataga
    20041 cttatgaatt tagaacaaga tgttttaata gttggaagtg gagtttcagg attatactgt
    20101 gcattaaatt tagataaaag tttaaatgtg ctagtagtat cgaaatcaac aattgaaaac
    20161 aataatacat atcttgcaca aggtggaatt tcaactgcta gaaatattga tgatatagaa
    20221 tcctttgtag aagatacaat gaaggctggg cagtataaaa atagagttga agcagttcaa
    20281 gtacttgcag atgaatctat agaaaatgta gggcagatag tagaatatgg tatgccttta
    20341 gacaaagaaa atggagagat agattacaca agagaaggag cacatagtgt aaatcgaata
    20401 gttcatagta aggataatac tggagaagtt gtttttaaaa ctttattaaa agaagctaaa
    20461 actagagaaa atataactct tatagaagat gcctatcttt tagatatatt aaaagatggt
    20521 aataaatgta taggagctag aatttttaaa tcgaaaaaag agattcatgt tttttcaaaa
    20581 atagtagttt tagctacagg tggaattggt gggcttttta aaaattctac taatcaaaga
    20641 catcttacag gtgatggaat tgcaatagct ttaagaaata acataaaaat agaaaattta
    20701 gaatatattc aaatacatcc tacagccttt tatgaagaaa ataatgaagg taggagaatg
    20761 cttatatctg aatcattaag aggagaggga gctaaacttt taaataaaaa taaagaaaga
    20821 tttgtggatg aacttcttcc tagagatgta gtttcaaaag ctatttttga acaaatggaa
    20881 aaagataaat taccttatgt gtatttagat gcaacacatt tagatagtga atatttaatt
    20941 aatagatttt cttttattta taatgaatgt ttagctagag gaacagatat aacgaaagaa
    21001 tgtataaagg tatctcctgc acaacattat tttatgggcg ggatacatgt agatttggac
    21061 tcaaaaacat ctatggaaaa tttatatgca gttggtgaaa ttagttgtac aggtgttcat
    21121 ggagccaata gattggcaag taattcatta cttgaagggc ttgtattttc aaaaagggca
    21181 gctaaaaata taaactcagt tatagataat gtaaaagtta agtttataga tgtaccagat
    21241 atggatatag atatagaaca ggtaaaaaaa gaaaataaaa tattagtcat aaaagaaatt
    21301 gaaagaacaa gtgaggattt tggcgatgaa ttatttgatt attgatagaa tgatacagga
    21361 tgctttgata gaagatgttc caagtgaaga tataacaact aattctattg tggatgaaag
    21421 ttctaaatct actgtagatt taatttgcaa acaagatggt ataatagctg gcttaggtgt
    21481 atttaagaga gtttttgaga tacttggtga tgtagatgta aaattatata aaaatgatgg
    21541 tgatagagta aagaatcgtg aaaagatagc tgtattaaca ggtagtacta gaaacttact
    21601 tgtaggagag cgtgtagcat tgaattattt acagagaatg agtggtatag ctactattac
    21661 aagtaggtat gttgaaaagc ttgagggtac aaatacaaaa ttattagata gtagaaaaac
    21721 gatacctaat cttagaatat tagataagta ttctgttaaa gtaggtggag gatgcaatca
    21781 tagatttaat ttatctgatg gaatattgct taaagataac catatggggg ctgctggtgg
    21841 tgttaagaac gcagttgaat tagctagaaa aaacacttct tttgttagaa agatagaagt
    21901 ggaagtggaa actttagaca tggtaaaaga agcaatagaa gctaatgcag atataataat
    21961 gttggataat atgagtttgg aaatggcaaa acaagcagta gatattataa atggaagagc
    22021 aattgtagaa ttttctggaa atgtaaattt agatactata gaagaaatag gaaaaattgg
    22081 tgtagatgtt gtttctgtag gagcgcttac acattctgtt aaagctctcg atataagtat
    22141 gaagaatttg agaaatatat aaattttata ttgaaataaa attaattaaa atagactcct
    22201 taaatttgtt ttataaattt aaggagtcta ttttttattc attaaaatta ataaaaatag
    22261 aaaaaaatca atcaataa
//
LOCUS       DAEPXF010000056        22113 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain HB27
            SAMN13376389-rid12479423.denovo.060, whole genome shotgun sequence.
ACCESSION   DAEPXF010000056 DAEPXF010000000
VERSION     DAEPXF010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13376389
            Sequence Read Archive: SRR10518432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 22113)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22113)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001069624.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001069624.1
            Genome Coverage       :: 315x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/15/2021 01:54:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,165
            CDSs (total)                      :: 4,076
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 89
            rRNAs                             :: 10, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 10 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 19 of 69
            Pseudo Genes (incomplete)         :: 44 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 10 of 69
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22113
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13376389-rid12479423.denovo.060"
                     /strain="HB27"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="China: Hebei"
                     /collection_date="2017"
     gene            complement(328..519)
                     /locus_tag="KRL67_001793"
     CDS             complement(328..519)
                     /locus_tag="KRL67_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH1522673.1"
                     /translation="MDDFSKLKNLYEDGYRCIYHDCVDNNYTIYLKNFYTEGSETIEL
                     SSESDFSQFKDYIDGLRMS"
     gene            738..1784
                     /gene="cobT"
                     /locus_tag="KRL67_001794"
     CDS             738..1784
                     /gene="cobT"
                     /locus_tag="KRL67_001794"
                     /EC_number="2.4.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide--dimethylbenzimidazole
                     phosphoribosyltransferase"
                     /protein_id="HBH1522674.1"
                     /translation="MSLLESISKNIYSLDNSSIEKTKQRLDRLIHPTGSLGKIEDICM
                     QLAGIFGNENFETSKKVIIAFAGDHGVYEEGVAPDPQNITKLQFPNFSKGLCGVGVIS
                     KFVGADVVAVDVGINCDEKLDGVLDYKIRKGTSNMAKGPAMSKQEAIRCLEIGIEIAE
                     QCIEKDYKVIGIGEMGIANTTPSTAIISVISGCDPLEVTGIGAGLKKERLKHKAEVIR
                     KAIEINNPNPTDGVDILSKVGGFEIGSMAGVILGCSANRIPVVIDGFISYAAALIAYK
                     INPKTREYMIASHLSAESGTKRALDILKLDPLLNMDMRLGEGSGAALAFDIIEASNYT
                     YKNMATFDEIDMGR"
     gene            1816..2376
                     /gene="cobU"
                     /locus_tag="KRL67_001795"
     CDS             1816..2376
                     /gene="cobU"
                     /locus_tag="KRL67_001795"
                     /EC_number="2.7.1.156"
                     /EC_number="2.7.7.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional adenosylcobinamide
                     kinase/adenosylcobinamide-phosphate guanylyltransferase"
                     /protein_id="HBH1522675.1"
                     /translation="MSKIILVTGGARSGKSNFAEKLCMDRNNNTAYIATSIPFDDEMK
                     DRVKKHQENRPINWNTYEIYEDIYSIIKEIYTGHETVILDCVTLLVNNLMFEHNLNIE
                     ESSQEEINKLEQYIKEQVVKLIKEIEKTNLYFVFVTNELGMGIVPGNKLSRIYADIVG
                     RINQYIASKSNEVYFVVSGIPMKIKE"
     gene            2386..3159
                     /gene="cobS"
                     /locus_tag="KRL67_001796"
     CDS             2386..3159
                     /gene="cobS"
                     /locus_tag="KRL67_001796"
                     /EC_number="2.7.8.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide-GDP ribazoletransferase"
                     /protein_id="HBH1522676.1"
                     /translation="MKRFILILQFLTRIPIKLNVGFDDEFYKSIVYFPLVGFVIGILS
                     YLIGWISMLLFEPFIASIIITLAGVLITGGLHIDGLGDTFDAIYSYRDKEKMLEIMKD
                     SRLGTNSLLAIMFVLLLKVGFIYNIISNNSLWVIIFMPMIARLGVMLLTYKTVTPREK
                     GMGNLFIGKLTTSMLITAIIYTLLIVGLIAKFIFLLPNIVLIKVLGSVIVVFVFIILF
                     KKHIYKKIDGVTGDILGCGIELSELVYLIYIYLLIFMFF"
     gene            3179..3793
                     /locus_tag="KRL67_001797"
     CDS             3179..3793
                     /locus_tag="KRL67_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HBH1522677.1"
                     /translation="MIRLILIRHALTNDNKKGKLSGHIDSCISEEGKLQINKITRYLS
                     NENIDKVYTTPSTRTKDTIEKISKLKLLEIEEKETLREISFGDFEGITFEEIKIKYPN
                     EFEKMIKEGNNYRYPNGESLIDSYKRVAKEIDNIILENDSNLDTKTILICSHAGTIRN
                     IITHLISGSYKYHWNFKIDNASITVLEIDGGFAVIDKMNFTDFI"
     regulatory      3860..4039
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            4169..5668
                     /locus_tag="KRL67_001798"
     CDS             4169..5668
                     /locus_tag="KRL67_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobyric acid synthase"
                     /protein_id="HBH1522678.1"
                     /translation="MLQGTASNVGKSVLVAGLCRIFKQDGYTVAPFKSQNMALNSFIT
                     KEGLEMGRAQVFQAEAAKIEPIADMNPVLLKPSGNNKSQVIVRGKVVGEMPSSKYHDY
                     KLELVDVLKETFNSLNSRYDIVVMEGAGSNAEINLKDRDISNMGMAEIADAPVIIVGD
                     IDRGGVFASLAGTMLLLNEDEKRRVKGVIINKFRGKKELLQDGVKMLEDIIKVPVIGV
                     VPYTDIKIEDEDSVTTRFRKKMNKSDIHIEIIRTPHMSNFTDFNIFETQEDVSIRYVD
                     YGESLGNPDILIIPGTKSTIDDLTYIRKSGLESQIKNLHSQGKLIFGICGGYQMLGRK
                     LKDPYHVESEIEEVDGIGLLDTETTFEKEKTTTQVEATIVECISEYMNNLKGKKVKGY
                     EIHMGITKRGNDVRSLDLITKKLNKEVNYTEGSVNKEGNVIGTYLHGIFDEISFTRAI
                     LNNIRKKKGLGELESKVNSFNEFKDKEYDKLADFLREHLDIEKIYEIMK"
     gene            5707..7083
                     /locus_tag="KRL67_001799"
     CDS             5707..7083
                     /locus_tag="KRL67_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobyrinate a,c-diamide synthase"
                     /protein_id="HBH1522679.1"
                     /translation="MKKILIAGTNSGVGKTTISLGIMQALVKRNMKVQPYKVGPDYID
                     PSYHTFITGRHSRNLDSYMLDDEKIKYIVNKSSKDADISVIEGVMGLYDGFGIDLDNC
                     TSSHTSKVLKSPVILVINGKSMAASSAAMVLGYKELDKDVNIKGVIVNNVKTNSHYQI
                     IKSSIEKYCNVEVLGYFPPNNKFSLESRHLGLVPSVEMKSLREKFYTLADEIEHYIDI
                     DRIIEISESDEFESDFDFQTLIENNKIKEHVNNKSIAIAYDKAFNFYYRENIELLEEL
                     GLEINYFSPLEDTSVPNADYIYIGGGFPEIFGKELEANESMRTSILEAHNNNIPIYAE
                     CGGLMYLGEKLLNQEEQEFNMVGIFSGISKMTSSLKRFGYCIGVAKENTLLSKKGESI
                     KGHEFHHSIFESDEKCVYSMRKERDGNIVEEWDGGYSKGNTLATYLHTHFYNNLNCIE
                     NFLKKGNE"
     gene            7084..8055
                     /locus_tag="KRL67_001800"
     CDS             7084..8055
                     /locus_tag="KRL67_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin biosynthesis protein"
                     /protein_id="HBH1522680.1"
                     /translation="MSILSIYIGYVTDLIVGDPYSFPHPVRFIGKLINFTQGIIRKVS
                     KNDRQLKLGGFILWFITVGITYLITYAIVKLFSFNILLSIVVNSFIIYTTLATKCLKD
                     EALKIYNVLKTEDIEKSRIQLSYIVGRDTTNLSEPEIIRATVETVAENTVDGIIAPMF
                     YAFIGGAPLAMAYKAINTLDSTVGYKNKKYKDIGFASAKIDDIANYIPARISVILMTI
                     GSFFLNYNYRNCFKISIRDRKNHKSPNCAFSEGAVSGALGIQLGGTNVYFGEKVYKPT
                     IGDKLREIDKEDIIKTNKIMYASSFVSMLVFTLIYVLCRMILINLII"
     gene            8087..9157
                     /locus_tag="KRL67_001801"
     CDS             8087..9157
                     /locus_tag="KRL67_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HBH1522681.1"
                     /translation="MKDLGHGANVDEMARLYGKNPKEIIDFSSNINPNVLPNLERYIL
                     KGLEECRNYPDINYTNLRENISKYIDINPDFIIPGNGATEVIYLLMKSIKKKLAIINP
                     TFSEYRRSAELNNLDIIDLELDLENNFKLNIDIIKENIKRFDSLFICNPNNPSGNVQD
                     LKELVHLLDKHNKVLIIDETFMEFVEDESKYSLVKYIESNKNIFIIKAVTKFFGMPGL
                     RLGYGLTSNTEIMNKIYEHKEPWTINSFADILSNFIFEDKEYIKSSKEYYIEERKYML
                     QELRNIRNIKVYDTDANFILIRTYKKTTKELKKDLFKQGNILVRDASNFIGLDDSFIR
                     VAIKSHEDNKILIENMKNLLGD"
     gene            9159..10046
                     /locus_tag="KRL67_001802"
     CDS             9159..10046
                     /locus_tag="KRL67_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin biosynthesis protein"
                     /protein_id="HBH1522682.1"
                     /translation="MKSYGICPASCGEFVQGIIDDEEYLCSYAIDMYSKVYIEEKLVD
                     INLGRYKSRLAIEKVFEKFNLPKKYTKNISLNINSKIPVGKGMASSTADIGATIKATL
                     SLIDKDLSSEEISKLAAEIEPTDSIFIDKNSIFNPLNGTVIKYLGNLTNAKVVILEPN
                     KVLDTMKIRLRQDYNRLKVENKEVIKKSFALLEEGLKKNNLSLVGEACTLSSLANENI
                     EKKEYLNEIIKISKKYGAYGVNIAHSGTVIGILTDKFMNDKKMIDALCESNINSVYNK
                     IYTLNIINGGIKGEIEWNT"
     gene            10034..10666
                     /locus_tag="KRL67_001803"
     CDS             10034..10666
                     /locus_tag="KRL67_001803"
                     /EC_number="5.4.99.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003724727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt-precorrin-8 methylmutase"
                     /protein_id="HBH1522683.1"
                     /translation="MEYIKNPMKIEEKSFELIQEIIDEIRPDYKFKNNIEEKIIKRAI
                     HTTADFDYLDILKISEEAVDSIIDALKNNASIYTDTNMALSGINKRKLESLGCKYKCL
                     VADDETAKLAKEKGITRSMAAVEIAAKEEGRKIFVLGNAPTALYKVMEMKSEGRLDLD
                     AVIGVPVGFVGAQESKDEVEKTDIPYIISKGRKGGSNLAAAIVNAILYTM"
     gene            10675..11901
                     /locus_tag="KRL67_001804"
     CDS             10675..11901
                     /locus_tag="KRL67_001804"
                     /EC_number="2.1.1.195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt-precorrin-5B (C(1))-methyltransferase"
                     /protein_id="HBH1522684.1"
                     /translation="MEEYVYIDGKKYRRGYTTGSCATGASKAAVYMLITKNRINTINI
                     DTPKGIPLLLKVDNINISDTFVECSIKKDGGDDIDATHTMDIYARAEIVSKNDKNKGY
                     LTLKDIDSLSTNSECKSELYKFIRVYGGTGIGVVTKKGLSVDVGKPAINPTPLKMINH
                     EIRKLIGDNFESILGNDKVLKITIFAPQGETVAKKTFNPRLGIVGGISIIGTTGIVEP
                     MSDEGWKKSLSIELQMKKEQGLDKIILVPGNHGEQFIREKLNLDIKYVVRVSNFIGYM
                     IKEAQRIGYKKILMAGHIGKFIKVSAGIFNTHSKVADARSEILVANLALMGARYEFLN
                     KINQCVTTEEAVELINSSEYREVYNILSNKCRERVKQYLNEDSDDIDVEVIIFSMDKS
                     LLGKSDNTDDLVEVFI"
     gene            11898..12506
                     /locus_tag="KRL67_001805"
     CDS             11898..12506
                     /locus_tag="KRL67_001805"
                     /EC_number="2.1.1.289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt-precorrin-7 (C(5))-methyltransferase"
                     /protein_id="HBH1522685.1"
                     /translation="MINIIGLGPGNLDYITKKGENLISTSDVLIGGKRNLESIKNFEG
                     EKIVLDSNLREIIEYINNNKEKQISIIASGDPLIYGIGRYLSKNIDNKMLNMVSGISS
                     LQYIFSKIYVDMNDVYITSSHGKVPDFDYILSHKKVCMVTDFKIGPKQISKEIIDRNL
                     NKIIVVGENLSYDNEKITIAKPEEIIRMDNFDMNVVVILDEE"
     gene            12496..13071
                     /locus_tag="KRL67_001806"
     CDS             12496..13071
                     /locus_tag="KRL67_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylating cobalt-precorrin-6B
                     (C(15))-methyltransferase"
                     /protein_id="HBH1522686.1"
                     /translation="MRNSEFITGKVPITKEEVRAISISKLNLVNAKNLIDIGAGTGSV
                     SVEAAYNYPNLKVISIEKNDDAIELIEKNIKKFNLKNIEVIKGYAPINLGLNVKVESI
                     FLGGTGNNLEEIIEWSKKNLVTGGRLVANFIIIDTFNQTLKLLKKHGFKEIDVCVLNV
                     SKLEKLGKGEYFKPLNPIYIISCEKGENDDE"
     gene            13064..13816
                     /locus_tag="KRL67_001807"
     CDS             13064..13816
                     /locus_tag="KRL67_001807"
                     /EC_number="2.1.1.271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt-precorrin-4 methyltransferase"
                     /protein_id="HBH1522687.1"
                     /translation="MNKVHFVGAGPGDKELITLKGYKLLSNADVVIYAGSLVNPELLE
                     YCKEDCQIHNSAHMDLQEIIDVMREGIENNKSVVRLQTGDFSIYGSIREQVEDLNKLN
                     IDYDCTPGVSSFLGAASSLGVEYTVPEISQSVIITRMEGRTPVPEKESIQSYAKHQTS
                     MVIFLSVQEIEKVVSKLLEGGYPKDTPIAVIYKATWADEKIVKGTLSDIAVKVKENNI
                     NKTALIMVGRFLGEEYNNSKLYDKDFKHEYRG"
     gene            13800..14921
                     /gene="cbiG"
                     /locus_tag="KRL67_001808"
     CDS             13800..14921
                     /gene="cbiG"
                     /locus_tag="KRL67_001808"
                     /EC_number="3.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt-precorrin 5A hydrolase"
                     /protein_id="HBH1522688.1"
                     /translation="MNIEGNIAIICITENGKKLAVKINSLVNNCIIYQVKNKESNLAV
                     EETCVHTVQKKLSDFVGEIFSKFDYIVFIMATGIVVRSIAPYIISKFSDPAILVMDEK
                     GKNVISLLSGHIGGANSLTQYISSLIDANPVITTATDINQKASLDMIAKKLNAHMDNF
                     RESVKEVNSMLVNNKCVGLYVDEGYQVDTRGFKLIESKGELRDSFSNTYIEKLVVVSN
                     KINCIEDIENLNLDNKLEHCYSTKKIIKVIPRDIVIGIGCRRNTDSELMYNSLEEFLN
                     KQNIDIRAINTVGSIELKKDEKAIIELSKILKVPFKTFSIDEISKVDNLFDKSDFVKK
                     NVGVYSVAEPVAYLLSKGNLLVEKHKYKGITFSVGRIKI"
     gene            14918..15643
                     /gene="cobJ"
                     /locus_tag="KRL67_001809"
     CDS             14918..15643
                     /gene="cobJ"
                     /locus_tag="KRL67_001809"
                     /EC_number="2.1.1.131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011701973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="precorrin-3B C(17)-methyltransferase"
                     /protein_id="HBH1522689.1"
                     /translation="MIYVVGIGPGSKDTMTLEAIKAIEDSEAIVGYKTYIKLIEEFIQ
                     GKEIIQNGMRQEVDRCKQAVEEAKKGKKVAVVSSGDAGIYGMAGLILELISKEDEDIK
                     VKVIPGVTASIGAAAILGAPIMHDFCHISLSDLMTPWEVIEKRLRLAAEADFVICLYN
                     PRSKGRSEHLSKAFKIMGEFKGGDTLVGVVKDVGREKEEKFVCTFENMDFEKVDMTTM
                     VIIGNKSTYINGELMITPRGYTV"
     gene            15640..16392
                     /gene="cobK"
                     /locus_tag="KRL67_001810"
     CDS             15640..16392
                     /gene="cobK"
                     /locus_tag="KRL67_001810"
                     /EC_number="1.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="precorrin-6A reductase"
                     /protein_id="HBH1522690.1"
                     /translation="MILILGGTSDSLSICDKINELKNQPYILSVTTDYGRKLAQQHAE
                     NVILGKLGKEDMLKFIKDNNIVKIIDATHPYAVEVSKTAITCAKLLNIDYIRFERKSL
                     IEEIDYDNKFIVDTIEEACEIANKIGTNIFIGTGSKNLNRFIEEISDKNLVVRVLPTS
                     EVILSCEKLGLSADNIIAMKGPFSQHINEETYKHYNIDLVITKESGIAGGFLEKVNAC
                     KTFNIPVIIIKRESINYETVFNDINKIKDILV"
     gene            16411..17889
                     /locus_tag="KRL67_001811"
     CDS             16411..17889
                     /locus_tag="KRL67_001811"
                     /EC_number="2.1.1.151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt-factor II C(20)-methyltransferase"
                     /protein_id="HBH1522691.1"
                     /translation="MKKALLVVSFGTSYHETREKTIDTCEDKIKNSLKSYDFFRAYTS
                     NMIINKIKKRDGIEIDNPIQALDKIYEQGYDEVIIQTLHIICGEEFNKLKEQVDGYSS
                     KFKKIVLGRPLLTHIEDYQEAVEAIKHQIPHMESNEAVVFMGHGTLHESHSAYPALEY
                     MLRDNGINAYVGTVEGYPEIEHVIRRLKEGNIKTVNLMPFMLVAGDHAINDMAGDEED
                     SWKTILEENGFDVRVHLKGLGENSYIQDKFVRHAVKCAENAKFYGIGAGPGDGSLLTI
                     KAVNTLKKLDILYTPESKKGGDSLALSIVSEYLPESVEIKSRHFPMSFDGNEKSLAWD
                     NVAQEIISDVNDGKNVGFVTLGDPMIYSTYVYIMKRILGEVEVETIPGISSFSNIASN
                     QNFPLVMDKEALVVIPCTMEDDKIEYALQNYNSIVLMKVYKNFKEIIKKLEKYDLIEH
                     AILVSNSSQESENVFTDLKEAHLEDKISYFSTILVNRDNKIK"
     gene            17919..18824
                     /gene="hemC"
                     /locus_tag="KRL67_001812"
     CDS             17919..18824
                     /gene="hemC"
                     /locus_tag="KRL67_001812"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HBH1522692.1"
                     /translation="MNIVVGTRGSNLALIQTEWVINELKKKYPEISFEIKIIKTKGDL
                     IQNVSLDKIGDKGLFVKEIEQQLLDGKIDIAVHSMKDMPSYLADGLKFAHTPKREDPR
                     DVLILREGYKNLDDLPHGAVIGTGSKRRKFQLLKQRPDLNIVQVRGNVETRIRKIKDE
                     NMHGIVLAASGIIRANLQDKISSYLPVDVVIPAPAQGALAIEIRSNDSTIEGIVNSLK
                     DENTEIQILAERGFLDGVNGSCHIPMAAYCEIIQDKIHLTGLYGDSEGKKVVIKSIDG
                     DISSPRELGLKLAKLVLKEYENYEG"
     gene            18814..20313
                     /gene="cobA"
                     /locus_tag="KRL67_001813"
     CDS             18814..20313
                     /gene="cobA"
                     /locus_tag="KRL67_001813"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HBH1522693.1"
                     /translation="MKGKVYLVGAGPGDYKLMTLKGLECIRKSDVIVYDRLANSSYLR
                     EAKPDCELIYVGKASSNHILPQEDINRIIAEKAKEGKIVTRLKGGDPYVFGRGGEEAE
                     TLVDEGIEFEVVPGITSAIGGLCYAGIPITHRDHASSFHVITGHLKGDDSGELNWNAL
                     ANNKGTLVFLMGISNLEKISENLMKEGKDKDTPVALISWATRYNQRVVTSTLENVYKT
                     AIKEEVKPPTLIVVGSVVGLREKLNFFERKSLFGKSIAVTRSRNQNSVLVEKIMDLGG
                     NPIEIPTIKIEKIQNNIKLENEIKNINKYNYLILTSKNAVEIFFEKIYEMNFDLRILS
                     NLKVCAIGSATSNELKKRGIIADIVPKKFVAESLYEELAPILNDNDNILIPRAENARD
                     YLVDKLKAICEVTEVHIYRTVIDEDKKGEILDILNSNDIDYITFTSSSTVNNLVEIIG
                     KENIKILEKIKTLSIGPITTETINRLGIKLYKESATSTIDSLVDLIIKN"
     gene            20330..21295
                     /gene="hemB"
                     /locus_tag="KRL67_001814"
     CDS             20330..21295
                     /gene="hemB"
                     /locus_tag="KRL67_001814"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HBH1522694.1"
                     /translation="MLRRPRRLRASKAIRNLVRETKVNVEDLIYPLFVVEGENIKEEI
                     KSLPDVYHLSIDMLEEEIKEISELGIEHIMLFGVPDEKDECASQAFNDNGIVQKAIRK
                     VKEINSDINVITDVCMCQYTSHGHCGILTDTGYVDNDKSLEYLAKISVSHAKAGADMV
                     APSDMMDGRIQAIREALDENGLENVGIMAYSVKYASNYYGPFREAAHSAPSFGDRKTY
                     QMDPANSNEAIIETELDILEGADILMVKPALSYLDIIRRVKDNFDLPLAAYNVSGEYS
                     MLKFAVKEGLLNEDVILESITSIKRAGADIIITYFAKDIAKLLKK"
     gene            21448..22026
                     /locus_tag="KRL67_001815"
     CDS             21448..22026
                     /locus_tag="KRL67_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional precorrin-2
                     dehydrogenase/sirohydrochlorin ferrochelatase"
                     /protein_id="HBH1522695.1"
                     /translation="MLYPINLKLDELDVVIIGGGEIAYRKCKNFLEFNKNVTIVSKQI
                     LNKFYDLKGNIKIIKDDYKEIYIKDASVIIAATNNRELNMEIGLYCRAKNKLVNVVDN
                     IEISNFTVPSYIKRGDLLISVSTGGKSPSLSSKIKKEIEEIYTEDYEEYLNVLGDIRK
                     EVIKKYEDKSKRKDLLNMLITLDLEELKKFDI"
BASE COUNT         9098 a         2122 c         4164 g         6729 t
ORIGIN      
        1 atttgatatt atatttgaaa ggtagatttt agtatatttt gaaaattttg ttttgaaaat
       61 ttgggattat ttaagaaatt ttgtattata aatagaagga aatagataaa ttgtatagaa
      121 taaaatatat taagataagt taataatgtt aaatgggaag tgagtttatg gaaaaaccaa
      181 atacaaaagt tgttaacttt caagagtata aagacagaaa aaaccaaaga gaagtaaata
      241 gagacgaact tttaaagctt ataaaaaaaa ttgtgacttc aaaataggga gttgttaaac
      301 tccctatttt aatgggtttc ataataatta agacattcta agtccatcta tataatcttt
      361 gaattgtgag aaatctgatt cactacttaa ttctatagtt tctgaacctt ccgtataaaa
      421 attcttaaga tagatagtat aattattatc aacacaatca tgataaatac atctatatcc
      481 atcttcatac aagtttttta acttagaaaa atcatccaca atatcatctc cttaaagttc
      541 ttttaaatat atttttacaa aaagcatcta attgtatgca aattgacaat gtaaaataaa
      601 ggaaattaac ataaaatgta gaataatttt tatatgtatg taaaaataaa aagaaaaagt
      661 tgaaattttt agaaaataaa agtatttttt atctaaaaag agaatttaaa aaataaaaaa
      721 atgttgggag gataaaaatg agtttattag aaagtatatc aaaaaacata tattctctag
      781 ataattcttc tattgaaaaa acaaagcaga gattggacag gcttatacat ccaactggaa
      841 gcttaggaaa gatagaagat atttgcatgc aattagcagg aatatttgga aatgaaaatt
      901 ttgagacaag taaaaaggtt ataatagctt ttgctggaga ccacggtgta tatgaagaag
      961 gagtagcacc tgaccctcaa aatataacaa aactacaatt cccaaatttt tcgaaaggtc
     1021 tatgtggagt tggagtaata agtaaatttg taggggcaga tgttgtagct gtagatgtag
     1081 gaataaattg tgatgaaaaa ctggatggag tattagatta caagataaga aaagggacat
     1141 caaatatggc aaaagggcca gctatgagta aacaagaagc gattagatgt ttagagatag
     1201 gtattgaaat agcagagcag tgtatagaaa aagattataa ggttattggt attggagaga
     1261 tgggtatagc aaatactact ccaagtacag caataatatc tgtaatttca gggtgtgacc
     1321 cattagaggt tactggtata ggtgcaggtc ttaagaaaga gagactaaag cataaggctg
     1381 aagtaattag aaaggctatt gaaattaata atccaaatcc aactgatgga gtagatattt
     1441 tatctaaagt aggaggattt gaaataggct caatggcagg tgttatatta ggttgtagtg
     1501 ctaatagaat ccccgtagta attgatggct ttatttctta tgcagcagca ttaattgcat
     1561 ataagataaa tccaaaaact agagaatata tgatagcatc ccatttgtca gcagaatcag
     1621 gaactaaaag agcattagat atattgaaat tagacccttt attaaatatg gatatgagat
     1681 taggtgaggg aagtggagct gctttagcat ttgatataat agaagcttca aattatactt
     1741 acaaaaatat ggcaactttt gatgaaattg acatgggaag ataaatataa aaatttaata
     1801 tgtaggagtg ttttgatgag taaaattatt ctagtaacag gaggggctag gtcagggaag
     1861 agtaactttg cagaaaaact atgtatggac agaaataata atacagctta tatagcaact
     1921 tctattccat ttgatgatga aatgaaagat agagtgaaaa agcatcaaga gaatagacct
     1981 ataaactgga atacctatga aatatatgaa gatatatatt ccataattaa agagatatat
     2041 acaggacatg aaactgttat attagattgt gtaacattat tagtaaataa tctaatgttt
     2101 gaacacaatt taaatataga agagtctagt caagaagaaa taaacaaatt agaacaatac
     2161 ataaaagaac aagttgtcaa acttattaaa gaaatagaaa aaacaaatct atactttgta
     2221 tttgtgacga atgaattagg tatgggaatt gttcctggga ataaacttag taggatttat
     2281 gctgatattg taggtcgcat aaatcaatat atagcatcaa aaagcaatga agtttatttt
     2341 gtagttagtg gcatacctat gaaaataaag gagtagtatg tgattatgaa aagatttatt
     2401 ttaatactac agtttttgac aaggatacct ataaaattaa atgtaggatt tgatgatgaa
     2461 ttttataagt caatagtata ttttccactt gtaggttttg tcataggtat attatcctat
     2521 ttaataggat ggatttctat gcttttattt gaaccattta tagcttctat aataataact
     2581 ctagctggtg tcttaattac tggaggactt catatagatg gattaggaga tacatttgat
     2641 gcaatatata gttataggga taaagaaaaa atgcttgaaa taatgaaaga ttcaagatta
     2701 ggtacaaatt cacttttagc aattatgttt gtacttttat taaaagttgg attcatctac
     2761 aatataataa gtaataattc attatgggtt ataattttta tgccaatgat tgcaaggcta
     2821 ggagtaatgc tgttgacata taaaactgtg acaccaagag aaaaaggaat gggaaattta
     2881 tttataggaa aattaactac aagtatgtta ataactgcta taatatacac attattaata
     2941 gttggtctta ttgctaaatt tatattctta ttacctaata tagtgttgat taaagtatta
     3001 ggttcggtta tagtagtttt tgtatttata atattattca aaaaacatat ttataaaaaa
     3061 attgatggag taacaggaga tatattaggc tgtggaatag aactttcaga gcttgtatat
     3121 ttaatttaca tatatttgtt aatttttatg tttttttaaa gtaaaaagtg gtgataatat
     3181 gataagatta atattaataa gacatgcact gacaaatgat aataaaaaag gaaaattatc
     3241 aggccatata gatagttgta ttagtgaaga gggcaaacta caaataaata aaattactag
     3301 atatctgagt aatgaaaaca tagataaggt ttatacaaca ccatctacac gtactaaaga
     3361 cacaatagaa aaaatatcaa aattgaaatt attagaaata gaagaaaaag aaactttaag
     3421 agaaattagt tttggagact ttgaaggaat aacttttgaa gaaattaaaa tcaaatatcc
     3481 caatgagttc gaaaaaatga taaaagaagg taacaactat agatatccaa atggagaaag
     3541 tttgatagac tcttataaaa gagtggcaaa agaaattgat aatataattt tagaaaatga
     3601 tagtaattta gatacaaaga caatacttat atgttctcat gcaggaacaa taagaaatat
     3661 aataacacat ttgatttctg gtagttataa gtatcactgg aactttaaaa ttgacaatgc
     3721 atctattaca gttttagaaa ttgatggtgg atttgctgta atagataaaa tgaattttac
     3781 agacttcata taatttaatc aaaaccaaac attacaatct gtgtttgtaa tatatctgta
     3841 aaattagcaa tgaaaataga atattagatt aaaattaggt tcttaattag atgttaaaag
     3901 ggaaaaaggt tagatgcctt tgcagccccc gctactgtga aaccaacgaa actataaata
     3961 ccactgtcag tttgatggga aggttataga agtaggatga agttaagcca ggatacctgc
     4021 ctaatttaat ttaaaacaag aggtcttcgg ggtgaaagat ttttaattaa tatatataaa
     4081 agtcctatct ttagaagtat taggactttt tttaatttgt tttttagtaa attttaacaa
     4141 ggagggatgg aaagtgggga aaaaaataat gctacaagga acagcttcaa atgtaggtaa
     4201 aagtgtctta gtagcaggtt tgtgtagaat atttaagcaa gatggttata ctgttgctcc
     4261 ttttaagtca caaaatatgg ctttaaattc ttttataact aaagaaggat tagaaatggg
     4321 aagagcacag gtatttcaag cagaggctgc gaaaatagag ccaatagctg atatgaatcc
     4381 agttttatta aaaccttctg gaaataataa aagtcaagtt attgtaagag gtaaagttgt
     4441 aggagaaatg ccttcctcta agtatcatga ttataaactg gagttagtag atgtgttgaa
     4501 ggaaacattt aatagcttaa attcaaggta tgatattgtg gttatggaag gtgcaggaag
     4561 taatgcagag ataaatttaa aagatagaga tatctctaat atgggtatgg cagaaatagc
     4621 tgatgcacct gtaataatag tcggagatat tgatagagga ggcgtatttg cctcattagc
     4681 aggaacaatg cttttattaa atgaagatga gaaaagaaga gtaaaaggtg taataatcaa
     4741 taaattcaga ggaaaaaaag agcttttaca agatggtgta aagatgctag aagacatcat
     4801 aaaagtacct gttataggag tagtacctta tacagatata aaaatagaag atgaagacag
     4861 tgtaactacg agatttagga aaaaaatgaa taaaagtgat atacacatag aaataattag
     4921 aaccccacat atgtcaaatt ttacagattt taatatattt gagacacaag aagatgtaag
     4981 tattagatat gtagactatg gagaatcttt gggaaatcca gatatattaa taatacctgg
     5041 aactaaaagt actatagacg atttaacata tataagaaaa agtggtcttg aatcgcaaat
     5101 caaaaattta catagtcaag gtaaattgat atttgggata tgtggtggat accaaatgtt
     5161 aggtagaaaa ttaaaagacc cttatcatgt tgaaagtgaa attgaagaag ttgatggaat
     5221 aggtctttta gatacagaaa caacgtttga gaaagaaaag actacaacac aagtagaagc
     5281 tactattgtt gaatgtatca gtgaatatat gaataacctt aagggaaaaa aagtaaaagg
     5341 ttatgaaatt catatgggca ttacaaagcg aggtaatgat gtaagaagtt tagatttaat
     5401 aactaaaaaa cttaataaag aagtgaatta tactgaagga agtgtaaata aagaggggaa
     5461 tgtaattggg acttatcttc atggaatatt tgatgaaata agctttacaa gagcaatttt
     5521 gaacaatata agaaagaaaa aaggtttagg agaattagaa agcaaagtaa actcttttaa
     5581 tgaatttaag gataaagagt atgataagtt ggctgatttt ttaagagaac atttagatat
     5641 agagaaaata tatgagatta tgaaataatg atgaccttta tttataattt aggtaggagg
     5701 tgtattatga aaaaaatttt aattgcgggt actaatagtg gagtaggtaa aacaacaata
     5761 tcattaggaa taatgcaagc tttggttaag agaaatatga aagtacagcc atataaagta
     5821 gggccagact atatagaccc ttcatatcac acttttataa ctggaagaca ttccagaaat
     5881 ttagattctt atatgttaga tgatgaaaaa ataaaatata tagtaaataa gtcctcaaaa
     5941 gacgcagata tttcagtaat agaaggtgtt atgggcttat atgatggttt tggaatagat
     6001 ttagataatt gtacaagttc acatacttca aaggtattaa agtcacctgt tatattagtt
     6061 ataaatggta aatctatggc agcatctagt gctgctatgg tacttggata taaggaatta
     6121 gataaagatg taaatataaa aggtgttata gtaaataatg taaagactaa tagccattat
     6181 caaataatta agagttctat agaaaaatat tgtaatgttg aagtattggg gtattttcct
     6241 ccaaataaca aattctcttt agaatctcga catctaggat tagttcctag tgtagaaatg
     6301 aaatcactaa gggaaaaatt ttatacctta gcagatgaaa ttgaacacta tatagatatt
     6361 gacagaataa ttgaaatatc agaaagtgat gaatttgaga gtgattttga ttttcaaact
     6421 ttgatagaaa ataataaaat aaaagaacat gttaataata aatcaatagc tatagcatat
     6481 gataaagcat tcaactttta ttatagagaa aatatagagc ttttagagga acttggttta
     6541 gaaataaact attttagccc tcttgaagat acatcagtgc caaatgcaga ttatatttat
     6601 attggaggag gttttccaga aatatttgga aaggaattag aagcaaatga gtcaatgaga
     6661 acttccatat tagaagcaca taacaataat atacctattt atgcagaatg tggaggactg
     6721 atgtacctag gtgagaaact attgaatcaa gaagaacaag aatttaatat ggttggaata
     6781 tttagtggaa ttagtaagat gacatcttct ttaaagagat ttgggtactg tattggtgta
     6841 gccaaggaaa atactctttt atctaaaaaa ggggaatcta ttaaaggtca tgagtttcat
     6901 cattcaatat ttgaaagtga tgaaaaatgt gtatattcaa tgagaaaaga aagagatgga
     6961 aatatagtag aagagtggga tggtggttat agtaaaggaa acactttggc tacatatctt
     7021 catactcatt tttataataa cttaaattgt atagaaaatt ttttgaaaaa aggaaatgag
     7081 taaatgagta ttttatctat ttatatagga tatgtgacag acttaattgt aggagaccca
     7141 tattcttttc ctcatccagt gagatttatt ggtaaactta taaattttac tcaaggtata
     7201 ataagaaagg tctctaaaaa tgataggcag ttaaaactgg ggggatttat attatggttt
     7261 ataactgttg gaataacata tttaataact tatgctatag tgaagttatt tagttttaat
     7321 atattattga gtattgtagt gaatagtttt ataatttata caacactagc cacaaaatgt
     7381 ttaaaagacg aagcattaaa aatctataat gtattaaaaa ctgaagatat agaaaaatct
     7441 aggattcagc tatcttatat agtaggtagg gatactacta atttaagtga acctgaaata
     7501 ataagagcaa ctgtagaaac tgttgcagaa aatactgtag atggtataat tgcgcctatg
     7561 ttttatgcat ttattggagg agcaccactt gctatggcct ataaagctat aaatacattg
     7621 gactcaaccg ttggatataa aaataaaaag tataaagata ttggttttgc atctgctaag
     7681 attgatgata tagccaatta tataccagct agaatatctg ttattcttat gacaattgga
     7741 agtttctttt taaattataa ctataggaac tgttttaaaa tctctattag agatagaaag
     7801 aatcataaaa gtccaaattg tgctttttca gaaggtgcag tatctggtgc tctaggaata
     7861 caactaggag gtaccaatgt ttattttggg gaaaaagtat ataagccaac aataggggat
     7921 aagcttaggg agatagataa agaagatata attaaaacta ataaaattat gtatgcatct
     7981 tcatttgtat ctatgttggt atttacacta atatatgtgt tatgtaggat gattttaatt
     8041 aacttaatta tataaattat tgtttgttaa cttaaggggg acatagatga aggatttagg
     8101 gcatggtgcc aatgtagatg aaatggcaag attatatggg aaaaacccaa aagaaataat
     8161 tgattttagt tcaaacataa atccaaatgt attaccaaat ttagagagat acattttaaa
     8221 aggattagaa gagtgtagga attatccaga tataaattat acaaacttga gagaaaatat
     8281 atcaaaatat atagatataa atccagactt tataatacct gggaatggtg caacagaagt
     8341 gatttatttg cttatgaaga gtattaaaaa aaaattggct ataataaatc cgactttttc
     8401 agagtataga agaagtgctg agttaaataa tttagatatt atagatttag agttagattt
     8461 agaaaataac tttaaactta atatagatat aataaaagaa aatataaaaa gatttgatag
     8521 tttatttata tgtaatccta ataatcctag tggtaatgtc caagatttaa aggaacttgt
     8581 tcatttacta gataaacata ataaggtact aataattgat gaaactttca tggaatttgt
     8641 tgaagatgaa agtaaatata gtttggttaa gtatatagaa tcaaataaaa atatatttat
     8701 aattaaagct gtgactaagt tttttggtat gcctggttta aggttaggat atggtcttac
     8761 aagcaacact gaaattatga acaaaattta tgaacacaaa gagccatgga ctattaattc
     8821 ttttgcagat atactatcta attttatttt tgaggataaa gagtatatta aaagtagtaa
     8881 agaatattac atagaagaaa gaaaatatat gttacaagag ttgagaaaca taagaaatat
     8941 aaaggtctat gatacagatg ctaattttat tttgataaga acatataaaa aaacaactaa
     9001 agaattgaaa aaagatttgt ttaagcaagg aaacatatta gttagagatg cgtctaattt
     9061 tataggatta gatgatagtt ttataagagt ggctataaaa tctcatgaag ataacaaaat
     9121 tcttatagaa aatatgaaaa atttattggg tgattaaaat gaaatcttat ggaatatgcc
     9181 cagcatcttg tggagaattt gtgcaaggta taattgatga tgaagaatat ttatgttcat
     9241 atgcaataga tatgtattct aaagtgtata ttgaagaaaa attagtagat ataaatttag
     9301 ggagatataa gtcaaggtta gcaattgaaa aggtatttga aaaatttaat ttacctaaaa
     9361 aatatactaa aaatatttct ttaaatataa atagtaaaat acctgttgga aaaggaatgg
     9421 caagttcaac tgctgatata ggagctacca taaaggcaac attatcattg atagataaag
     9481 atttaagtag tgaggagatt tctaaattag cagcagaaat agaaccaaca gattctatat
     9541 ttatagataa aaatagtata tttaatcctc taaatggaac tgttataaaa tacctaggaa
     9601 atctgaccaa tgcgaaagtt gtaatacttg aaccaaacaa agtactagat actatgaaaa
     9661 ttagattgag gcaagattat aatagattaa aagtagagaa taaagaagtc ataaaaaaat
     9721 catttgctct tttagaagaa ggattgaaaa aaaataattt atctctagtt ggagaggctt
     9781 gcactttgag tagcttagca aatgagaata tagaaaaaaa agagtattta aatgaaataa
     9841 taaaaatatc aaaaaaatat ggagcatatg gagtaaatat tgctcacagt ggaactgtaa
     9901 taggtatttt aactgataag ttcatgaatg ataaaaagat gatagatgca ttatgtgaat
     9961 ctaacataaa ttctgtctac aataaaattt atacactaaa cataattaat ggaggaatta
    10021 agggggaaat agaatggaat acataaagaa tcctatgaaa atagaagaaa aaagttttga
    10081 attgatacaa gaaataatag acgaaattag accagattat aaatttaaga ataatatcga
    10141 agaaaaaata ataaagagag ctatacatac tactgcggat tttgattact tagatatatt
    10201 aaaaatatca gaagaagcag ttgatagtat aatagatgca ttaaaaaata atgcaagtat
    10261 ttatactgat acaaatatgg ctcttagtgg tataaataaa agaaagttag aatcattagg
    10321 atgtaagtat aaatgtttag tggcagatga tgaaactgca aaattagcta aagaaaaagg
    10381 aattactcgt tctatggcag cagtagaaat tgcagctaaa gaagaaggaa gaaaaatatt
    10441 cgttttaggt aatgcaccaa cagctttata taaagttatg gagatgaaat cagaaggtag
    10501 attagattta gatgcagtta taggagttcc tgtaggtttt gtaggagcac aagaatcaaa
    10561 agatgaagtt gaaaaaacag atataccata tataatttct aaaggtagaa aaggtggcag
    10621 taatttagca gcagcaatag taaatgctat attatatact atgtaggtac ctatatggaa
    10681 gaatatgtat atatagatgg taaaaaatat agaagaggat atactacagg ttcatgtgca
    10741 acaggtgcat ctaaggctgc tgtatatatg ttgattacaa aaaatagaat caatactata
    10801 aatatagata ctcctaaggg gataccatta ctactaaaag ttgataatat aaatatttct
    10861 gatacatttg tggaatgttc cataaaaaaa gatggtggag atgatataga tgcaactcat
    10921 accatggata tttatgcaag agctgaaatt gtatctaaaa atgataagaa caaagggtat
    10981 cttactctaa aggatataga tagtttaagt accaatagtg agtgtaaaag tgagctctat
    11041 aagtttatta gggtttatgg cggaactggt attggtgtgg ttacgaaaaa agggcttagt
    11101 gtggatgttg gtaaacctgc tataaatccg actccactta aaatgataaa tcatgaaata
    11161 agaaagctta taggagataa ttttgaatct atacttggaa atgataaagt tttaaaaata
    11221 acaatctttg ctccacaagg agaaactgta gcaaaaaaaa catttaaccc aagattaggt
    11281 atagttggag gaatatctat aataggtaca actgggattg ttgaacccat gagtgatgag
    11341 ggttggaaga agtctctatc tatagaactt caaatgaaaa aagaacaagg cttagataaa
    11401 ataatattgg ttccaggaaa tcatggagaa cagtttataa gagaaaaatt aaacttagat
    11461 attaagtatg tagttagggt aagtaatttt ataggctata tgattaaaga agctcaaaga
    11521 ataggatata aaaaaatctt aatggcgggt catataggta aatttataaa agtatctgct
    11581 ggtattttta atactcatag taaggtagca gatgctagaa gtgagatatt agtggcaaac
    11641 ttagctttaa tgggagctag atacgaattt ttaaataaaa taaatcaatg cgtaactact
    11701 gaagaagctg tagagctaat aaatagtagt gaatatagag aagtatataa tatattgagt
    11761 aataaatgta gggagagagt aaagcaatac ttgaatgaag attcagatga tattgatgtg
    11821 gaagtaatca tattttcaat ggataagtct ttattaggta aatctgataa tacagatgat
    11881 ttagtggagg tctttatatg ataaacataa taggtctagg acctggcaac ttagactata
    11941 taacgaaaaa aggtgaaaat ttaatttcca cttctgatgt attaattggt ggtaagagaa
    12001 atttagagtc tataaaaaac tttgaaggag aaaaaatagt cttagattct aatttaagag
    12061 aaattataga gtacataaat aacaataaag aaaagcaaat atcaataata gcatctggtg
    12121 acccattaat atatgggata ggtagatatc tatctaaaaa tattgataat aagatgctaa
    12181 atatggtttc tgggattagt tcattacagt acatattttc aaaaatatat gtagatatga
    12241 atgatgtata tattacaagt agccatggta aagtaccaga ttttgattac atattatcac
    12301 ataaaaaagt atgtatggtt acagacttta agataggacc aaaacaaata agtaaagaaa
    12361 taatagatag aaatctaaat aaaattattg ttgtaggaga aaatttgtca tatgataatg
    12421 aaaaaataac aatagcaaaa ccagaagaaa taataagaat ggacaatttt gatatgaatg
    12481 ttgtggtgat tttagatgag gaatagtgaa tttataacag gtaaagttcc aattactaag
    12541 gaagaggtta gagcaatatc aataagtaag ttaaatttag taaatgctaa aaacctcata
    12601 gacatagggg ctggtacagg aagtgtaagt gttgaagctg catataatta tcctaattta
    12661 aaagttatat ctatagagaa aaatgatgat gctatagagc ttatagagaa aaatataaaa
    12721 aaattcaact taaaaaatat agaagtgata aaaggatatg ctccaattaa tctagggtta
    12781 aatgttaaag ttgagtctat ttttcttggg ggaacaggaa ataatctgga agaaataata
    12841 gaatggtcta aaaaaaatct cgttacagga ggaagacttg tagctaattt tataataata
    12901 gatacattta atcaaacttt aaaactgctt aaaaagcatg gatttaaaga aatagatgta
    12961 tgtgttttga atgtttctaa actagagaaa ctaggaaaag gtgaatattt taaaccacta
    13021 aatcctatat atataatatc ttgtgaaaaa ggggaaaatg atgatgaata aagtacattt
    13081 tgtaggagca ggacctggag ataaagagct gataacatta aaaggataca aattattaag
    13141 taatgcagat gtagtgatat atgcaggttc acttgttaat cctgaacttt tggaatactg
    13201 taaagaagat tgccaaatac ataatagtgc acatatggat ttacaagaga taatagatgt
    13261 tatgagagaa ggtatagaaa acaataaatc tgttgtaaga ctgcaaacag gtgatttttc
    13321 tatatatggt tcaattagag aacaagtgga agatttaaat aaattgaaca tagattatga
    13381 ttgtactcct ggagtaagtt cttttttagg ggcagcatca tctttggggg ttgaatatac
    13441 agttccagaa atttctcaaa gtgtaattat aacaagaatg gaaggcagaa ctccggttcc
    13501 agagaaagaa tcaatacaat catatgctaa gcatcagaca tctatggtaa tatttttatc
    13561 tgttcaagaa attgagaaag ttgtgtctaa attactggaa ggtggatatc caaaagatac
    13621 tcctatagca gttatataca aagcaacttg ggcagatgaa aaaatagtta aaggaacttt
    13681 aagtgatata gctgtaaaag ttaaagaaaa caacataaat aagactgctt taataatggt
    13741 tgggagattt ttaggagaag agtataataa ttcaaagtta tatgataagg atttcaaaca
    13801 tgaatataga gggtaatatt gctataatct gtattacaga aaacggaaaa aaattagctg
    13861 taaagataaa ttctctagta aataactgta ttatatatca agtaaaaaat aaagaaagta
    13921 atttagctgt tgaagaaaca tgtgtacata cagttcaaaa gaagttaagt gattttgtag
    13981 gagaaatatt tagtaaattt gattacatag tatttataat ggctacaggt atagttgtac
    14041 gaagtatagc gccatacata ataagtaagt ttagtgaccc tgcaattttg gtaatggatg
    14101 aaaaaggcaa aaacgtaata agtttattga gtgggcatat tggaggggca aacagcttga
    14161 cacagtacat aagtagtctt attgatgcta atccagtaat aacaacagct actgatataa
    14221 atcaaaaagc ttccttagat atgatagcaa aaaaactaaa tgcacatatg gataatttta
    14281 gagaaagtgt aaaagaagta aattctatgc tagtgaataa taaatgtgtt ggattatatg
    14341 ttgatgaagg atatcaagtt gatactaggg gatttaaact tatagaaagt aagggtgagt
    14401 taagagatag tttttcaaac acgtatatag aaaaattagt tgtagtaagc aataaaataa
    14461 attgtattga agatattgag aatttaaacc tagataataa acttgaacat tgttatagta
    14521 ctaaaaaaat aataaaggta attccaagag atatagtgat aggaattggc tgtagaagaa
    14581 acacagatag tgagctgatg tataattcac ttgaagagtt tcttaataaa caaaatatag
    14641 atattagagc aataaacaca gttggaagta tagaactgaa aaaagacgaa aaagcaataa
    14701 ttgaattatc aaaaatatta aaagtgccat ttaaaacatt ttcaatagat gaaatatcta
    14761 aggttgataa tctttttgat aaatctgatt ttgttaaaaa aaatgtgggc gtgtattcag
    14821 tagcagaacc tgtagcatat cttttgagca aaggtaattt gttagttgaa aaacataagt
    14881 ataaaggaat aactttttca gtggggagaa taaaaatatg atatacgttg taggaattgg
    14941 accaggaagt aaagatacaa tgactttaga agcaataaaa gcaatagaag attctgaggc
    15001 tattgttgga tataagactt atataaaact aatagaagaa tttatacaag gtaaagaaat
    15061 aatccaaaat ggtatgaggc aagaagttga tagatgtaaa caagcggtag aggaagcaaa
    15121 aaaaggaaaa aaagtagcag ttgtaagcag tggtgatgct ggaatatatg ggatggcagg
    15181 tcttatttta gaattaatat ctaaagaaga tgaagatata aaagtaaagg taattccagg
    15241 tgttactgca agtattggtg cagcagctat actcggagcg cctattatgc atgatttctg
    15301 tcatataagt ttgagtgatt taatgactcc atgggaagta attgaaaaaa gattgagatt
    15361 ggctgctgaa gctgattttg taatttgttt gtataatcca agaagtaagg gaagaagtga
    15421 acatctaagc aaagcattta aaataatggg tgagtttaaa ggtggagata cacttgttgg
    15481 agtggtaaaa gatgtaggta gagaaaaaga agaaaaattt gtatgtactt ttgaaaatat
    15541 ggactttgaa aaagtcgata tgactacaat ggttataata ggaaataagt ctacctatat
    15601 taatggtgaa ttgatgataa ctccaagagg ttatacagta tgattttaat tttaggagga
    15661 acttcagata gtttatctat atgtgataag ataaatgaac tgaaaaatca accatatatc
    15721 ctttcagtga ctacagatta tggaaggaaa cttgcacaac aacatgcaga gaatgtaatt
    15781 ttaggtaagc ttggaaaaga agatatgcta aaatttataa aagataataa tatagtaaaa
    15841 ataatagatg ccactcatcc ttatgcagtt gaagtatcca aaactgctat tacttgtgca
    15901 aaattattga atatagatta tataaggttt gagagaaaat ctctaattga agaaatagat
    15961 tatgataata aatttatagt agatacaatt gaagaagcct gtgaaatagc aaataaaata
    16021 gggacaaata tattcatagg aacaggaagt aaaaatttga acagatttat agaggaaata
    16081 agtgataaaa atttagttgt tagagttctt ccaacgagtg aagtcatatt aagttgtgaa
    16141 aaattaggtt taagtgcaga taatataata gctatgaaag gtccatttag ccagcatata
    16201 aatgaagaaa catataaaca ttacaatata gatttagtta taacaaaaga aagtggaata
    16261 gctggtggat tcttagagaa ggtaaatgca tgtaaaactt tcaatatacc agtaataatt
    16321 ataaaaagag aatcgattaa ctacgaaact gtatttaatg acataaataa aattaaagac
    16381 atacttgtgt aatttttagg aggagaatat atgaagaaag ccttattagt agtgagtttt
    16441 ggtacaagct atcatgaaac gagagaaaag acaatagata catgtgaaga taaaataaaa
    16501 aattcattaa aaagctatga tttttttaga gcctatacat caaatatgat aataaataaa
    16561 ataaaaaaaa gggatggaat agaaatagat aatccaatac aggctttaga taaaatatat
    16621 gagcaaggat atgatgaagt aataatccaa acactccata ttatatgtgg cgaagaattt
    16681 aacaaattaa aagaacaagt agatggatat tcttctaaat tcaaaaagat tgtattagga
    16741 agacctttgc ttacacatat tgaagattat caggaagctg tagaggcaat taagcatcaa
    16801 attcctcata tggaaagtaa tgaagcagta gtatttatgg gacatggaac acttcatgaa
    16861 tcacattcag catatccagc attggaatac atgcttaggg ataatggaat aaatgcttat
    16921 gttggtacag ttgaagggta tccagaaata gaacatgtaa taagaagatt aaaagaagga
    16981 aatataaaaa ctgtaaattt gatgccattt atgttagttg caggagacca tgctataaat
    17041 gatatggcag gagatgaaga agattcttgg aaaacaatat tagaagaaaa tggatttgat
    17101 gtaagagttc atttaaaagg attaggagaa aattcttata tacaagataa atttgttcgt
    17161 catgctgtaa aatgtgcaga gaatgctaag ttctatggta taggagcagg accaggagat
    17221 ggttctttgc ttacgataaa agcggttaat actttaaaga aactggatat tctatacact
    17281 ccagaatcaa aaaagggtgg agatagtcta gctttatcaa tagttagtga atacttacct
    17341 gaatcagtgg aaataaagtc taggcatttc ccaatgagtt ttgatgggaa tgaaaaaagt
    17401 ttagcttggg acaatgtggc acaagaaatt atatcagatg ttaatgatgg taaaaatgta
    17461 gggtttgtta cattaggaga ccctatgatt tatagtacat atgtatatat aatgaaaaga
    17521 atattaggag aggtagaagt cgaaactata ccaggaattt catcattttc caatatagca
    17581 tctaatcaga attttccatt agtcatggat aaagaagcct tagtggtaat tccttgtact
    17641 atggaagatg ataagataga gtatgctttg caaaattata attcaatagt attgatgaag
    17701 gtgtataaaa atttcaaaga aataataaaa aaattagaaa agtatgattt aatagaacat
    17761 gctattttag taagtaattc ttcccaagaa agcgaaaatg tgtttacaga tttaaaagaa
    17821 gcgcaccttg aagataagat atcttatttt tctactatac tagtaaatag agataataaa
    17881 ataaaatagg tgaaagcaat atttaaagga gacttaatat gaatatagtg gttggaacta
    17941 ggggtagtaa tctagcgctt atacaaacag aatgggtaat aaatgaatta aaaaagaagt
    18001 atccagaaat tagttttgag attaaaatca taaagacaaa aggagattta attcaaaatg
    18061 tttcactgga taaaataggt gataaaggat tgtttgtcaa agaaatagag caacaattat
    18121 tagatggaaa aatagacata gcagtacata gtatgaaaga tatgccttct tatttagcag
    18181 atggactaaa atttgctcat acgcctaaga gagaggaccc aagagatgtt ctaatactta
    18241 gagaaggata taaaaattta gatgatttgc ctcatggagc agtaatagga acaggaagta
    18301 aaagaaggaa gtttcaatta ttgaaacaaa gacctgactt aaatatagta caagttagag
    18361 gtaatgtgga aactaggata cgaaaaataa aagatgaaaa tatgcatggt attgtgttgg
    18421 cagcatcagg aataattaga gctaatttac aagataaaat aagttcttat ttacctgttg
    18481 atgttgttat tccagcacct gcccaaggag ctttagctat agaaattaga agtaacgata
    18541 gtacgataga gggaatagta aattcactga aggatgagaa tacagaaatc caaatattag
    18601 ctgaaagagg ttttttagat ggtgtaaatg gaagttgtca tattccaatg gcagcttatt
    18661 gtgaaataat ccaagataaa atacatctta ctggcttata tggagattca gaaggaaaaa
    18721 aagtagtaat caagtctata gatggagata tttcttctcc tagagagtta ggtttaaagt
    18781 tagctaaact tgtattgaag gagtatgaga attatgaagg gtaaggttta cttagtaggg
    18841 gcaggaccag gtgattataa actcatgact ttaaaaggtt tagagtgtat aagaaaatcg
    18901 gatgtaatag tatatgatag attagcaaat agtagttatt taagagaagc taaacctgat
    18961 tgtgaactta tatatgtagg caaggcatca agtaatcata ttttgccaca agaagatata
    19021 aatagaatta tagcagaaaa agctaaagaa ggaaaaattg taactcgtct taaaggagga
    19081 gacccttatg tatttggtag agggggagaa gaagcagaga ctttagtaga tgaaggtata
    19141 gaatttgaag tagttccagg tataacttct gctataggag gtttatgtta tgcgggtatt
    19201 cctataacac atagagacca tgcttcgtca tttcatgtaa taacaggtca tttgaagggt
    19261 gatgatagtg gagaattaaa ttggaatgct ctcgcaaata acaaaggtac tcttgtgttt
    19321 ttgatgggaa tttcaaacct agagaaaata agtgaaaact taatgaaaga aggaaaagat
    19381 aaggatacac ctgttgctct tataagttgg gcaactagat ataatcaaag agttgttaca
    19441 tctacacttg aaaatgttta taaaactgct attaaagaag aagtaaaacc accaactctt
    19501 atagtagttg gaagtgttgt aggacttaga gaaaagttga acttctttga aagaaagtct
    19561 ttatttggaa aaagtattgc tgtaactagg tctaggaatc aaaatagcgt attagtagaa
    19621 aaaataatgg atttaggtgg aaatcctata gaaataccta ctattaagat agaaaagata
    19681 caaaataata ttaaattaga aaatgaaata aaaaatataa ataagtataa ttatctaata
    19741 ctaactagta aaaatgccgt agaaatcttc tttgaaaaga tatatgaaat gaattttgat
    19801 ttaaggatac tatctaattt aaaggtatgt gctataggtt ctgctacatc aaatgagttg
    19861 aaaaaaagag gaattatagc agatatagta ccaaaaaaat ttgtagcaga gtcactgtat
    19921 gaagagttag cacctatttt aaatgataac gataatatac ttataccgag agcagaaaat
    19981 gctagagatt atctggttga caaacttaag gctatttgtg aagtaacaga agtacatata
    20041 tatagaacag taatagatga ggataaaaaa ggtgagatat tagatatttt aaattctaat
    20101 gatatagatt atataacatt tactagttcg tcaacagtaa ataatcttgt tgaaattata
    20161 ggaaaagaaa atattaaaat attagaaaaa ataaaaacac tatctatagg gcctataact
    20221 acagagacaa taaataggtt gggaataaaa ttgtacaaag aatctgctac atctactata
    20281 gattcgttag tagatttaat tataaaaaat taattttgga ggcttgaata tgttaagaag
    20341 accaagaaga ctaagagcaa gtaaagcaat cagaaatttg gtaagagaaa ctaaggtgaa
    20401 tgtagaagat ttaatatatc cactgtttgt agtggagggg gaaaatataa aagaagaaat
    20461 taaatcttta ccagatgtct atcatttatc aatagacatg ttagaagaag aaataaaaga
    20521 aataagtgag ttgggaattg aacatataat gttatttggt gttccagatg aaaaagatga
    20581 gtgtgcttca caagctttta atgataatgg aatagttcaa aaggcaatta gaaaggtaaa
    20641 agaaataaat tcagatataa atgtaataac agatgtctgt atgtgccaat atacaagtca
    20701 tggacattgt gggattttaa ctgatactgg ttatgttgat aatgataaat cattggagta
    20761 tttggctaaa atatctgtaa gccatgctaa agcaggagct gatatggttg ctccatctga
    20821 tatgatggat ggaagaatac aagcaataag agaagcttta gatgaaaatg gattagaaaa
    20881 tgttggaatc atggcttata gtgttaagta tgcttcaaat tattatggac cttttagaga
    20941 agcagctcac tcagcaccat cttttggaga tagaaaaaca tatcaaatgg acccagcaaa
    21001 tagcaatgaa gctataatag agactgaact tgatatattg gaaggtgcag atatacttat
    21061 ggttaaacct gcattatcat atttagatat tataagaaga gttaaagata attttgactt
    21121 acctctagca gcttataatg taagtggaga atactctatg cttaagtttg ctgtgaagga
    21181 aggtttatta aatgaagatg taattcttga atcaattaca tctataaaaa gagcaggagc
    21241 agatataata ataacttatt ttgctaaaga cattgctaaa ctgttgaaaa aataaattct
    21301 agtagtataa agaatatcaa aaagtatagg aattgtaatt aactattata cagtagtttt
    21361 gtttagacat ttattgaaga ataatagaaa ctaatttgca atttctatac tttatttatg
    21421 taactaaata aaatttggag gtagaaaatg ctatatccta ttaatttaaa attagatgag
    21481 ttagatgtag ttataattgg tggtggagaa attgcatata ggaaatgtaa aaatttttta
    21541 gagtttaaca aaaatgttac catagtttct aaacaaattt taaataaatt ttatgattta
    21601 aaaggaaata taaaaataat aaaagatgat tataaagaaa tatatataaa agatgcttct
    21661 gtaataattg ctgccacaaa taatagagag ttaaatatgg aaataggatt atattgtaga
    21721 gcaaaaaata agctagtgaa tgtagttgat aatatagaaa tatcgaactt tactgttcca
    21781 tcatatataa aaagaggaga tttacttata agtgtatcta ctgggggcaa gagtccttca
    21841 ttatcatcaa agataaaaaa agagattgaa gagatatata ctgaagatta tgaggagtat
    21901 ttaaatgtat taggagatat aagaaaagag gttataaaaa aatacgaaga taaaagcaaa
    21961 agaaaagatt tgcttaatat gttgataaca cttgacttag aagaacttaa aaagtttgat
    22021 atataaaaaa taaaataaaa aatgttaaaa aggtgttgac ggattatttt taagatgata
    22081 tagtattact tgtccttaag aaaagggcaa aca
//
LOCUS       DAEPXF010000057        21707 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain HB27
            SAMN13376389-rid12479423.denovo.061, whole genome shotgun sequence.
ACCESSION   DAEPXF010000057 DAEPXF010000000
VERSION     DAEPXF010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13376389
            Sequence Read Archive: SRR10518432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 21707)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21707)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001069624.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001069624.1
            Genome Coverage       :: 315x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/15/2021 01:54:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,165
            CDSs (total)                      :: 4,076
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 89
            rRNAs                             :: 10, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 10 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 19 of 69
            Pseudo Genes (incomplete)         :: 44 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 10 of 69
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21707
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13376389-rid12479423.denovo.061"
                     /strain="HB27"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="China: Hebei"
                     /collection_date="2017"
     gene            87..1010
                     /locus_tag="KRL67_001816"
     CDS             87..1010
                     /locus_tag="KRL67_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBH1522696.1"
                     /translation="MEIRVLRYFLAIAREESISGAATFLHITQPTLSRQIMELEEELG
                     VKLLVRGNRKITLTNEGRLLHKRAEEIISLVDKVELEFDNPNEIISGDIYIGSGETEA
                     MRLIAKTAHHFHQKYPNVRYHLFSGNADDVTEKLDKGLLDFGILIEPANIQKYDYTQI
                     PATDVWGLLMRKDSALASQNTINPKDLCNIPLIISRQTLVDKAISKWLQGSNEKLSIV
                     ATYNLVYNASLLVEEGLGYALCLDRLINTTENSNLCFRPLSPPFEAKLNIIWKKHQIF
                     SKAAEKFLLQIQKDFSSIMLDTSKLDTPKEK"
     gene            complement(1182..2324)
                     /locus_tag="KRL67_001817"
     CDS             complement(1182..2324)
                     /locus_tag="KRL67_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HBH1522697.1"
                     /translation="MDFKIEKYLLKKAEELAFITIKHGGEFKLKSYEVPKGGLDVPIK
                     NEVLVKGIKEKTAQDKLNSMSIADAMIYIIGIDSKFKNNAEYEKFLNALSKDIDLDLK
                     SYMGYMSRKYFEIGEHTDSLIYIKAFITMYPDDLDAMYNYAIVCQEIAKQYQKDMDDK
                     AMNAFLLEAMAKLEKVIDVDENFALGYYHLGYHYYNQGQYLKTKLTWEEALRLGLDAD
                     LVAEVQENLGKMDFKVQYEEGYTLVFQGKFKEGLEKLLPLEEEHMDWWNLLFMIALGY
                     KGMGEIEQAKMYLEKILIIKPNQVDTIVELGLCEAYKNNLDKAIEYFEQAAKIKEDPE
                     ILCNLGMAYLNNGDIDDATYYIERAYELNPQDEITIACLRELGINK"
     gene            complement(2342..4096)
                     /locus_tag="KRL67_001818"
     CDS             complement(2342..4096)
                     /locus_tag="KRL67_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="B12-binding domain-containing radical SAM
                     protein"
                     /protein_id="HBH1522698.1"
                     /translation="MKILLTTLNSKFIHTNLAIRYLKEFVRDLIEVSMKEYTINNDLD
                     YILKDIYKNEYDIILFSTYIWNVGDIVKLCDNLKKIRPNTKIALGGPEVSYDSYEAMK
                     KYDFVDYILYGEGELIFRDLVLHLQGKMEINDVNGLVYRQGNEIIVNKPMELLQNLDE
                     IPSPYENLNPKEYENRIVYYESSRGCPFNCQYCLSSTIPGLRYFSLDRIKSDLKALID
                     ARVSQIKFIDRTFNANRKVAMEIMDFLMKNDNGYTTYHFEVTAYLIDDKMLEFLADCK
                     EGLFQFEIGVQSTNEKTLDAVGRRDDFKKLSHVVQTVASYRNIHQHLDLIAGLPYEDY
                     KSFENSFNDVFNLGIEHLQLGFLKMIKGTGMRKVADEHGFKYKDYAPYEFLYNNYISY
                     EETLKLKDIEDILERYYNSKNFVLSMRYIIGRFYKQSPFKFFEVFAKYFDENGYFDLA
                     QGKNQLYKILMDFYNEKINIDNDVFNDILKYDYISLGKTSNMPQFFNKLDMDDFKNRC
                     HVFLQDNDNLSTYLPSFVDKPAKHIIKYVHFEPFRFNVVDLKNDINTEIREVENVVLF
                     EYDDKKIFEKSKTHKVEI"
     gene            4232..4438
                     /locus_tag="KRL67_001819"
     CDS             4232..4438
                     /locus_tag="KRL67_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH1522699.1"
                     /translation="MELIEKVSINSLSKRDLLLIIKSLEYTNENTQISDFIDLRNNIV
                     KELCFLTDTKEKDFLDYLEKNSTL"
     gene            complement(4443..5366)
                     /locus_tag="KRL67_001820"
     CDS             complement(4443..5366)
                     /locus_tag="KRL67_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH1522700.1"
                     /translation="MNDELLKKVYTSSFSSASRALKDLTIINTKSLIDDKTQRCVYID
                     ENRLRDELIYYRFYGEKIGNINFLNILLPLILSNTNIQKSEDEVIKLIKKYVIYFKKE
                     KLLFDYLLGSVVYNSIMHNLINNSKIEYVELLQSIKDKIIGFSIELDKSDVVRFQMAR
                     IKAIQLIDKYIDLKSEDYDEESILLNVLNVLYDVYMEDRTVENEGINSIKKSILSILG
                     EDSKLNEDNIDFIFSMSEYVVKLRKYKIGVKAYNKSIDPRSLIRLEEGNTIIDPIFNQ
                     ITVMSKTFNDNILSIKINSKSGIYILKFKKV"
     gene            complement(5620..7320)
                     /locus_tag="KRL67_001821"
     CDS             complement(5620..7320)
                     /locus_tag="KRL67_001821"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HBH1522701.1"
                     /translation="MQDFKVAISNCLKEKIEDLSKEEIEALIEVPPNKDMGDYAFPCF
                     KLAKVFRKAPNMIASELAESIEPSGEITKVIQLGGYVNFFVNKSQLAETVIKKVLDEK
                     ENYGHSDFGKDKTVIVEYSSPNIAKPFHIGHIRTTVIGNALYKIYDSQGYKTIRINHL
                     GDYGTQFGKLIVAFKKWGEKEVVESNPIPELLKLYVRFHDEAEQHPEMEDEARAWFNK
                     LENGDEEAQELWQWFRNESLKEFNRVYKLLDIEFDSLAGESFYSDKMNRVIELLEEKN
                     LLKESKGARIVDLEEYKMPPALITKNDGSTLYMTRDLAAAIYRKETYDFDKCIYVVGS
                     QQNLHFQQWFKVIELMGYDWAKDLIHVGFGMVALEEGTMSTRKGRVVFLEDALNQAID
                     KTKDIILAKNPNAKNVDEISKQVGVGAVVFQELSNSRIKDYTFSWERTLSFDGETGPY
                     VQYTHARCCAVLRKAEVEVTSDIDYSLLADEDSAEVLRVIESFNKNILLALKKNEPHI
                     VTRFMLDLAQAFNKFYHDNPILVENLEIRKARLALVLATKQTLENSLKLLGMHAPERM
                     "
     gene            complement(7679..8092)
                     /locus_tag="KRL67_001822"
     CDS             complement(7679..8092)
                     /locus_tag="KRL67_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF523 domain-containing protein"
                     /protein_id="HBH1522702.1"
                     /translation="MILVSACLIGINCKYSGDNNENEKVKEYLKGKEFTLVCPEQLGG
                     LSTPRPPAEIVDGKVITKDNKDVTENFVRGANETLRIANLYVCSEAILKEGSPSCGCN
                     LIYDGSFSGKKIPGKGITARLLEEKGIKVKSEKDL"
     gene            complement(8167..10545)
                     /locus_tag="KRL67_001823"
     CDS             complement(8167..10545)
                     /locus_tag="KRL67_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HBH1522703.1"
                     /translation="MNEKSLRVLEYNKIIDLLKKKASSSLGLKYIENLVPNTDFVEVK
                     SMLEETSEAQSIIIKRGSVGLEGIHDIEDKVKRAYIGASLDPGSLIMIADTLRVARRL
                     RNSLSSSDEEDFNYPIIQSLSNSLYVYKDIEDQIYNAIISEVEISDNASSTLRDIRRR
                     IAQKNQSIRSKLNSIISSTTYQKYLQDAIISLRGDRFVVPVKAEYRSQVAGIVHDQSS
                     SGATLFIEPMTIVEMNNELRQLKLGEQEEIERILSELSAMVGEVSEDLISNQEILGRL
                     DFAFSKGKLSIQMRGIEPTLNEDKYLNIKNGRHPLLDKKKVVANTIYLGRDFHTLVIT
                     GPNTGGKTVTIKTVGLFALMTQSGLHIPVDYGSSMCVYDNVFADIGDEQSIEQSLSTF
                     SSHMTNIVSILQNVTADSLVIFDELGAGTDPVEGAALAIAVLEDINSVGAKCIATTHY
                     SELKNYALTKSGVENAAVEFDIETLSPTYKLLIGVPGKSNAFEISRKLGLSDYVISRA
                     KEYINTENIALEDVLQNVEKNRIKAVEDREEAERLKEEIERLKVEYDEKLEKLVSQRD
                     KMIEKAKSEAFSIIRQAKEEVDIIIKELRSLEQERASKEKNRKIEELRKELTSSMGSL
                     QPTVKSMIVPKVSNKEIKDLKPGEEVKVITLNQNGSVVSVDKKRKEAVVQIGIMKMTL
                     PFKSLQKTRKDVSTNVTKSTRNIIRSKSGSVKNEVDLRGLNLEEAIMEVEKYLDDAYV
                     AGLESVTVIHGIGTGVLKAGLQDILRRNRHVKSQRGGQYGEGGAGVTIVKLK"
     gene            10863..12314
                     /locus_tag="KRL67_001824"
     CDS             10863..12314
                     /locus_tag="KRL67_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-histidine dipeptidase"
                     /protein_id="HBH1522704.1"
                     /translation="MGNVLEGLKPESVFKNFEKISQIPRGSGNEKGISDFLLSFGKNL
                     GLETIQDESLNIIIRKPATKGYENCPGVVLQGHMDMVCEKEKNVEHDFLKDPIKLRID
                     GDMIYATGTTLGADNGIAVAMGMAILEDNTLEHPALEVLVTVNEEDGMNGADALDPSL
                     IKGQYILNMDSEEEGYLLVSCAGGKTCVVSLPVEYKKVEGDKQGLLVEVTGLLGGHSG
                     MEIVLQRANANKAIARVLSVLNVDYELASVDGGTKHNAIPREAKCVIAVNKADVESAK
                     KQINNILTAFKHEFTTSDPGMTYSIAETSVDKVLTKDCKEKVVQMSCLTPHGVQSVSL
                     DIEGLVESSTNFAIIETKESTIEFLTSVRSSVMSIRDEIADRIRLLAQALGANYDLIA
                     QYPAWEFKKGSKLEKICSETYEKLTGKVPTVMALHAGLECGLLLDKLPHAEAISIGPD
                     MFDVHTPNEHVSIPSVANVWDYVIEILKSMNQY"
     gene            complement(12518..15031)
                     /locus_tag="KRL67_001825"
     CDS             complement(12518..15031)
                     /locus_tag="KRL67_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HBH1522705.1"
                     /translation="MEDCVVNLKKLLLLIIVITSIFTSCIFSFDINAIGNDNRTIRVA
                     YPIQKGLTEIDEQGNYYGYTYEYLLEIAQYTGWNYEFVQVPGEINESITKLMKMLENG
                     EIDIMGGMLYNEPMQKIYNYAGNSYGVANTVLQALYENTDIDNIMSSQDVKKIRIAVI
                     ENSKTRLKELDEYCKINSMSPELVYCKTNDEQLEALKNGKADVLLNVSMNQIEGVRTV
                     AKFSPRPFYFITPKGSTDLTQNLNSAINNIEQKDPYFSTTLYEKYFTPENNQMFLSDE
                     ETNYIKDTEKLKVGVLLNKPPFQNIDKDTGELRGISIDLLNHICDKTGLEFELVPVKS
                     QEDLENMTKNHEIDIVAGMIYDYEYSREHNVAMSRPFISTQYTMMINNKLNENSLDGK
                     RIALTKGINHDGQFEENIVWYDNLEECIEAVNKGYADYTYGEGYSMQYYMNQPKYKNV
                     RLVPQTYKAKSICFGIIKKDKQELLNIFNKAIISISSEKMQSIVYQNTTYRQDFSLLY
                     IMQQNPIETIFIVASLLICIIGFMAFGLHTRTSMNEKISLELKKHTQLYEMTSEYFFE
                     YDYKQDKLIISMPERGIETYYGLDKNDKEGENGNTTNEIDKIRREYNKKFFNVIKSQE
                     NGTTEMYSIMFDDTFHWIRITSKIIYNDRKIPMYVIGKICNIDSEKEEKNRLLDKAQR
                     DSLTNIYNAAYIRKLTAQNLLNLSTKKNGALLIIDIDYFKSINDTYGHLVGDKVLIDV
                     AKALDESSANDDIVGRLGGDEFSVYINDIINKEELINKCNDVYNHIHFIKLPNGESLS
                     ISMGVAISRRGQEYDELYQIADNALYDSKRQGRNRFKIV"
     gene            complement(15254..16075)
                     /locus_tag="KRL67_001826"
     CDS             complement(15254..16075)
                     /locus_tag="KRL67_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HBH1522706.1"
                     /translation="MIDINLEKCVGCGMCESDCLVNAIKVKDDKAKVKNILCINCGHC
                     MAICPTDAIEMQGFDKNEVIEYNRESFELEPEKLLNFIKFRRSIRQYKDIEVEEEKIK
                     NIVEAGRYTPTGGNRQPIRYILVKEKLKEVKELAIEGLYNLALDTDDNDPVRSMYKNT
                     FKKMYKRYKENGNDSLFYDAPLLMVVVGDISLGGSAYVDGGLAASNMELMAYSQGLGI
                     CYNGFFVMASNVEPKIKELLGMSENEAVITSFILGYPDVKYKRTVNRNTAKFEMR"
     gene            complement(16528..17004)
                     /locus_tag="KRL67_001827"
     CDS             complement(16528..17004)
                     /locus_tag="KRL67_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase"
                     /protein_id="HBH1522707.1"
                     /translation="MKLTTICYIEKDDKTLMLYRNKKKDDIHEGKYVGVGGKFEQGET
                     PEECVIREVKEETGLTLKSLSYKGLITFPKFKDEEDWYMFLYFSDEFEGELSEKDLND
                     CKEGNLIWVDNDKIFDLNMWEGDMLFLNWAKTGNIFSAKIVYDNGKLKDYNVSFFD"
     gene            complement(17038..17616)
                     /locus_tag="KRL67_001828"
     CDS             complement(17038..17616)
                     /locus_tag="KRL67_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HBH1522708.1"
                     /translation="MREEALEILFKYLETDRMRKHCYAVEAVMRDLAKRLEPEKEEEW
                     AVAGLLHDLDSDIVGRKPGEICEGHATTTVELLKKENFGDEEMYRAILGHHDGMGVTR
                     TSLMEKAIYAADPITGFITAIALVYPDKKLTSVKTKSVIKRMKETRFASNVNRDAMRS
                     IEDIGIPFDEFAELSLNAMKNISDVLESAENN"
     gene            complement(17720..20302)
                     /locus_tag="KRL67_001829"
     CDS             complement(17720..20302)
                     /locus_tag="KRL67_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HBH1522709.1"
                     /translation="MKKIELLAPVGSFDSLKAAVQNGANAVYLGGKDFSARASANNFD
                     KNELIEAVKYSHIRGVQVFVTVNTLIKQYEIEDFVEYVKFLYDIDIDALIVQDIGMAR
                     LIKKLLPDFELHASTQMVAHSLEDVKYLQSVGFDRVVLARELNVDEIKWICENTTVDI
                     EVFVHGALCVCYSGQCLMSSMIGNRSGNRGRCAQPCRQKYELIDINTGEVVKSNGDYL
                     LSPRDLSTIEDLDKIIEAGVLSLKIEGRMKKPEYVATVVSGYREAINEFVDNKKISIS
                     EEIINNLYTIFNRKFTKGYILGEVGKDIMNSNRPNNQGLYIGKVLDYNRKNKRLRVML
                     ENTLKKGDGINLGGGTIGRIIKNGEITTIAHKGDLIELDFVGEAKRNQIIYKTSDSEL
                     MGRVQATFQQDKEFIKTNISAKVSIKLGKKPELFISDFCGNKSVVEGDKIVEKAIKVA
                     LSKEKIETQIQKLGNTPYKLKEIEIDLDEGVSMPISVLNQMRRDCIELLDEERIKIKD
                     RKFKKNRLKYQPNRVNKQKKLDTVPKIRVKVKNLEQLEAVLSYDVDLIYYEDINTLSK
                     ALQMVSNISTDSSISMDLDTSSSLGIPKELMGSNTNNIDTLSNTSKIDTLNKNHSNTA
                     VTDKKLIYSAPRIVRNKEYKQFEILDNIKGINKVQIGNWGSMEYFKKKDLNIDCYLNA
                     FNSESINHFKEEGASTVCLSQELNLNEIKQTLKYTDVEVEAVIYGYAPMMVTEYCPMG
                     VVVRDCKKDKRVAKCKESNYALRDFTDASYRITQDIFCRSTIYNSKVTCMLDNLYELE
                     EIGIDVFRIDFTVEDSEMVKKVIEAHIEVLSNNYKLGKNATDLYKKLDEIGTVAGHYY
                     KGVE"
     gene            complement(20833..21516)
                     /locus_tag="KRL67_001830"
     CDS             complement(20833..21516)
                     /locus_tag="KRL67_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1275 domain-containing protein"
                     /protein_id="HBH1522710.1"
                     /translation="MTSKSQMSESFFLCSLLAITGGFLDIYTYVVRGKVFANAQTGNI
                     VLFGLNIAEGNVKESFYYLIPILAFMLGVFIAEKIRKYFTNNKTKIHWRQVIIIIEMI
                     TILIVSFVPRGRFNMFVNVAISFICSMQVESFRKVNGNSFATTMCTGNLRSATETLFH
                     YIHTKNIFMIKKSLQYYAIIIFFISGAILGTFFTKIFVEKSILICFFILAVAFGIMFI
                     NKDDSFNEN"
BASE COUNT         6935 a         3855 c         2356 g         8561 t
ORIGIN      
        1 tctatattaa atacttaact atacttaaaa agcatataaa ttgtataatt atataataaa
       61 taagttaata ttgaggaggg ataattatgg aaataagagt attgagatac tttttagcta
      121 ttgcaagaga agaaagcata tctggtgcag caaccttttt acatataaca caaccaacac
      181 tttctagaca aattatggaa cttgaagaag agttgggagt taaattattg gttcgaggaa
      241 atagaaaaat caccttaaca aatgaaggta gacttttaca taaaagagca gaggaaatta
      301 tatcattagt tgataaggtt gaattagaat ttgacaatcc caatgagatt attagtggtg
      361 atatatatat aggaagtggt gaaactgaag ctatgcgact tatagctaaa actgcccatc
      421 attttcatca aaaatacccc aatgtaagat accacctttt tagtgggaat gctgatgatg
      481 tgactgaaaa attagacaag ggactgcttg attttggtat tttaattgaa cctgcaaata
      541 tacagaagta tgattataca caaattcctg ctactgatgt atggggttta ctaatgcgta
      601 aagatagtgc ccttgcatca caaaacacaa taaatccaaa agatttatgc aatatacctc
      661 ttattatttc aagacaaaca cttgttgata aagcaatttc taaatggctt caaggcagta
      721 atgaaaaact aagcattgtt gcaacataca atttagttta taatgcttct cttttggtag
      781 aagaaggtct gggctatgct ctgtgtttag atagactcat caatactaca gaaaatagta
      841 atctttgttt tagacctcta tctcctcctt ttgaagctaa attaaatatt atatggaaaa
      901 aacatcaaat attttcaaag gcagcagaaa aatttttact tcagatacaa aaagattttt
      961 cctctatcat gttggataca tctaagttgg atacacctaa agaaaaataa ataataataa
     1021 aaagtgagag tgtctcaaaa tggacttttt acttcatgag gcactctttt ttatatgaag
     1081 taaaatatat attcatcatt tcagcactaa aaaagaggct tatctctatt ttgagatagc
     1141 cccacttaac caataaaatt aaatacaacc taacaatatc cttacttatt tattccaagt
     1201 tctcttaaac aagcaatcgt aatttcatct tgtggattta attcataagc tctttctata
     1261 tagtaagttg catcatcaat atcaccatta tttaaatagg ccattcctaa attgcaaagt
     1321 atttctgggt cttcctttat cttagccgct tgttcaaaat actctattgc tttatctaaa
     1381 ttattcttat aagcctcaca aagccccaat tccactatag tatccacctg attaggcttt
     1441 attatcaata tcttctcaag atacatctta gcctgctcta tttcgcccat acccttatat
     1501 ccaagtgcta tcataaacag caagttccac caatccatat gttcttcttc taatggaagt
     1561 aacttctcta atccttcttt aaacttacct tggaatacaa gtgtataccc ttcttcatat
     1621 tgaactttaa agtccatctt gcccagattc tcttgtacct cagctactaa atcagcatct
     1681 aaccctagcc ttaacgcctc ttcccaagtc aacttagtct ttaaatattg accttgatta
     1741 taataatggt agcctaagtg ataataacct aatgcaaaat tctcatcaac atcaattacc
     1801 ttttcaagct tagccatggc ttcaagtaag aatgcattca ttgccttatc atccatatct
     1861 ttttgatact gtttagctat ctcttggcaa acaatcgcat agttatacat tgcatctaaa
     1921 tcatctggat acattgttat aaatgctttt atatatatta aagaatctgt atgttcacca
     1981 atctcaaagt attttctcga catatatccc atatacgatt tcaaatccaa atcaatatct
     2041 tttgataaag catttaaaaa cttttcatac tctgcattgt ttttaaactt actatctatt
     2101 ccaattatat atatcattgc atctgctata gacattgaat ttaacttatc ttgagcagtc
     2161 ttttctttta tgccttttac taatacttca ttttttatag gtacatctaa tccacctttt
     2221 ggtacttcat agctttttaa tttaaactct ccaccatgtt ttatagttat aaaagctaat
     2281 tcctctgctt tttttagcaa atatttttct atcttaaaat ccattttatc ctcctaaata
     2341 cttaaatctc tactttatga gttttagatt tctcaaatat ttttttatca tcatactcaa
     2401 ataagactac attttctact tctcttattt ctgtatttat atcatttttt aaatccacaa
     2461 cattaaatct aaatggctca aaatgaacat attttattat atgtttagct ggtttatcaa
     2521 caaaactagg taaatatgtt gataagttat cattatcttg taaaaataca tggcatctat
     2581 tcttaaaatc atccatatct aacttattaa aaaactgtgg catatttgat gttttaccca
     2641 gtgatatata gtcatatttt aatatatcat tgaatacatc attatctatg ttgatttttt
     2701 cattatagaa atccatcaat attttatata actgattctt tccttgtgcc aaatcaaagt
     2761 agccattttc atcaaaatac ttggcaaaaa cctcaaagaa tttaaaaggg ctttgtttat
     2821 aaaatctgcc tataatatat ctcatagata acacaaagtt tttagaattg tagtatctct
     2881 ccagtatatc ttctatatcc tttagcttca aagtttcttc atagcttata taattattgt
     2941 ataaaaactc atatggagca taatccttat atttaaaacc atgctcatct gcaacttttc
     3001 tcatacctgt ccctttaatc atctttaaaa agcctaattg cagatgctct atacctagat
     3061 taaatacatc attaaaagaa ttttcaaagc tcttataatc ttcataaggc agtcctgcaa
     3121 ttaagtctaa gtgctgatgt atatttctat aagatgctac agtttgtaca acatgagaca
     3181 actttttaaa atcatccctt ctacctactg catcaagtgt tttttcattt gttgactgaa
     3241 cacctatttc aaactggaaa agtccttctt tacaatcagc caaaaactca agcatcttat
     3301 catctatcaa atatgccgta acttcaaaat gataagttgt atatccatta tcgtttttca
     3361 taagaaagtc catgatttcc atagccactt ttctatttgc attgaatgtc ctatcaataa
     3421 actttatctg tgatactctt gcatctatta aagcttttaa atcactcttt atcctgtcta
     3481 agctaaaata ccttagacct ggaatagtag atgataaaca gtactggcag ttaaaaggac
     3541 aacctcttga tgattcataa tacactattc tattttcata ttcttttgga ttcaagtttt
     3601 cataaggact aggaatctca tccaaatttt gaagtaattc cataggctta ttcactatta
     3661 tctcattgcc ttgtctatat acaagtccat ttacatcatt tatttccatc tttccttgca
     3721 agtgaagtac taagtctcta aaaattaatt ctccctctcc ataaagtata taatccacaa
     3781 aatcatactt tttcattgcc tcataactat cataactcac ttccggtccg cccaaagcta
     3841 ttttagtatt aggtctaatt ttttttagat tatcacaaag cttaactata tctcccacat
     3901 tccagatata tgttgagaac aatattatat catattcgtt tttatatata tccttcaaaa
     3961 tatagtccaa atcgttgttt atcgtgtatt ctttcatact aacttctatc aaatctctta
     4021 caaattcttt taaatatctt atagccaaat ttgtatgaat aaacttagaa tttaatgttg
     4081 ttaataaaat tttcaaatat ttctcacctc tttttaagat tatactgtct ttttgtaccc
     4141 cttataggtt aaaatagtat atgtaaagtc taaataatat ctatataatt aatttattaa
     4201 tttcaagtaa tgtgaggaaa ggaagttttt tatggaatta attgaaaaag tatctatcaa
     4261 ctctttatca aaaagagatt tactacttat tataaagtct cttgaataca ctaatgaaaa
     4321 tactcaaatt agtgatttca ttgaccttag gaataatata gtaaaggaat tatgtttttt
     4381 aactgacact aaggaaaaag attttttaga ttatctagaa aaaaatagca cactctaata
     4441 acctaaacct ttttaaactt taatatataa atacctgatt tagaatttat ttttatgctt
     4501 aatatattat cattaaaggt tttcgacata actgttattt gattaaatat agggtctatt
     4561 atagtattac cttcttcaag tcttataaga cttcttgggt ctatgctttt attatatgcc
     4621 ttaactccaa ttttatattt tctcaattta acaacatatt cggacataga aaaaataaaa
     4681 tctatattat cttcatttaa tttactatct tcacccaaaa tggataatat agattttttt
     4741 atgctattaa ttccttcatt ttctacagtt ctatcttcca tatatacatc atatagaaca
     4801 tttaaaacat ttaataaaat actttcttca tcataatctt cagatttcaa atcaatgtac
     4861 ttatcaatta attgtattgc tttaatcctt gccatctgaa accttacaac atctgatttg
     4921 tctaattcta tagaaaatcc tattatcttg tctttaatac tctgtagtaa ttcgacatat
     4981 tctattttac tattatttat aagattgtgc ataatggaat tatacactac agaacctaat
     5041 aaataatcaa ataaaagttt ttctttttta aaataaataa catacttttt tattagttta
     5101 ataacctcat cttcgctttt ttgaatatta gtatttgata aaattagtgg taataaaata
     5161 tttaaaaaat tgatatttcc tattttttct ccataaaatc tatagtaaat aagttcatct
     5221 ctcagtctat tttcgtctat atacacacat ctttgcgttt tatcatctat taaggatttt
     5281 gtatttatta tagttaaatc tttaagagcc ctacttgcac tactaaaaga agatgtgtag
     5341 acttttttta gtaattcgtc attcataaat atatcctttc tgaaaaattg aatgtaaata
     5401 aattgttatt aaaaaaaaag agacagcccg tttaagaagc tgtcatatat agggggatag
     5461 tagtgtacaa aaatcattgt aatctactat ctatatatta ttatacgata ctactttttt
     5521 atatgtaaag catcagtttt cgacatccct atattttaca gttatacaaa ttgcgtacta
     5581 agttcaattt tattcttttt atttcaacat tttatcatgc tacattcttt ccggagcgtg
     5641 cattcccaac aatttcagag aattttctaa agtttgtttt gttgctaaaa ctaatgcaag
     5701 tcttgctttt cttatttcca aattttctac taaaattgga ttgtcatgat aaaatttatt
     5761 aaacgcttga gctaaatcta gcataaatct agtcactata tgtggctcat ttttctttag
     5821 agcaagtaaa atattcttat tgaatgattc tataactcta agaacttctg cactatcttc
     5881 atcagctaat agactataat ctatatcaga agtcacttct acttctgctt ttcttaaaac
     5941 tgcacagcat ctagcatgtg tatactgaac atatggtcca gtctcaccat caaaactaag
     6001 tgttctttcc caagagaaag tatagtcttt aattctacta ttagataact cttggaatac
     6061 tactgcccca acacctactt gcttagatat ttcatccaca tttttagcat ttgggttttt
     6121 agctagtata atatccttag tcttatctat agcttgattt aaagcatctt ctaaaaatac
     6181 gactcttcct tttctagtcg acatagtacc ttcttcaagt gcaaccatac caaatccaac
     6241 atgaattaaa tcttttgccc aatcatatcc cataagctca attaccttaa accattgttg
     6301 gaaatgtaag ttttgttgag aaccaactac atatatacac ttgtcaaaat cataagtttc
     6361 ttttctatat attgcagcag ctaaatctct tgtcatatat aatgttgaac catcattctt
     6421 agtaataagt gctggtggca ttttatactc ttctaaatca acaattcttg ctcctttaga
     6481 ctcttttaaa agatttttct cttctaatag ttcaatgact ctgttcattt tatcagaata
     6541 aaaactctct cctgctaaag aatcaaattc tatatctaaa agcttataca ctctgttaaa
     6601 ctcttttaaa ctctcatttc taaaccattg ccataattct tgtgcttctt cgtcaccatt
     6661 ttctaattta ttaaaccatg ctcttgcttc atcttccatt tctgggtgtt gttcagcttc
     6721 atcatggaat cttacatata attttagtaa ttctggaatt ggattagact ctactacttc
     6781 tttttctccc cactttttaa aggccactat taacttacca aactgagttc catagtcacc
     6841 taaatgattt attcttatag tcttgtatcc ttgggaatca tatatcttat ataaagcatt
     6901 acctataact gtagttctta tgtgccctat atggaaaggt tttgctatat ttggagaaga
     6961 atattcaact attacagttt tatcttttcc aaaatcacta tgtccgtaat tttccttttc
     7021 atctaaaact tttttaatta cagtttctgc taattgagat ttatttacaa agaaatttac
     7081 atatccgcct aattgtatta cttttgttat ctctccactt ggttctatac tttccgccaa
     7141 ttcacttgct atcatgtttg gagcttttct aaacactttg gctaatttaa agcatggaaa
     7201 agcatagtcc cccatatcct tatttggtgg aacttctata agtgcctcta tctcttcttt
     7261 cgataaatct tctatttttt ctttaaggca atttgatatt gccaccttga aatcttgcat
     7321 tctgttgctc ctctctacat tgttcctggt ttatattgtg cttatctctt tatgttaaat
     7381 caaatttcgt gttttttcaa gtatttcatt acagtgtaaa acactttaat aattatgttt
     7441 tctgtatata ttaaaaatac tataattgca gtttttaatc actctattat tgatttttta
     7501 taagactttt ttctatttta ttgtttctta tactatccag ttgactttat atactttttt
     7561 tactctttta tcaattttca tatatcaatg ttaactttat atactttttt tactctttta
     7621 tcaattttta tacatcaacg ttaactttac atactttttt tactcttcta tcaatttttt
     7681 ataagtcttt ttcactctta acttttattc ctttttcttc taatagcctt gctgttatac
     7741 ctttccctgg tattttttta cctgaaaaac taccatcata tattagatta catccacaag
     7801 aggggctacc ctcttttagt attgcttcgc tacatacata taaatttgct attcttagag
     7861 tttcattagc ccctcttaca aagttttctg ttacatcctt attatcttta gtaattactt
     7921 ttccatcaac tatctcagca ggtggtcttg gtgtagatag accacctaac tgctctgggc
     7981 atacaagtgt aaattctttg ccttttaaat attccttgac tttttcattt tcattattat
     8041 caccactata tttacaattt attcctatta aacatgcaga taccaatatc ataaaaatgt
     8101 gctcctatac aaattcttaa tatattctca tacaaattac taatgtattc ttatacaaat
     8161 tacaaattat tttaatttaa caattgttac tcctgctcca ccttctccat attgaccacc
     8221 tctttgtgat tttacatgtc tatttcttct taaaatgtct tgtagccctg cttttaatac
     8281 tcctgtacct attccatgta ttactgttac agactctaaa cctgccacat atgcatcatc
     8341 aaggtacttt tctacttcca ttatagcttc ttctaggttt aatcctctaa ggtcaacttc
     8401 attttttaca ctaccagatt ttgaccttat aatgtttctc gttgatttag ttacatttgt
     8461 tgaaacatct ttgcgtgttt tttgtaaaga tttaaaaggt agggtcatct tcattatacc
     8521 aatttgcact actgcttctt ttctcttttt atctactgaa actacactac cattttgatt
     8581 taaagttatt accttaactt cttcacctgg ctttaagtct tttatctcct tatttgatac
     8641 ctttggaact atcatacttt ttactgttgg ctgaagactt cccatagaac tggtcaattc
     8701 ttttctaagt tcttctatct tacgattctt ttcttttgaa gctctttctt gttcaagact
     8761 tctaagttct tttattataa tatctacttc ttctttagct tgtcttataa ttgaaaatgc
     8821 ttctgattta gctttttcaa tcatcttatc tctttgactg accaattttt caagtttttc
     8881 gtcatactct actttcagtc tttcaatctc ttcttttaat ctttcagctt cttctctatc
     8941 ttctactgct ttgattctat tcttttcaac attctgtaac acatcttcca aagctatgtt
     9001 ttctgtattt atatattctt tagcacgtga aatcacataa tcacttaatc caagttttct
     9061 agaaatctca aacgcatttg attttccggg aacacctata agtaacttat atgttgggct
     9121 taaagtttct atatcaaatt caactgctgc gttttctaca cctgatttag taagagcata
     9181 gtttttaagc tcactatagt gagtggtagc tatacattta gctcctacag aatttatatc
     9241 ttctaatact gctattgcaa gggcagctcc ttcaacaggg tctgttcctg ctcctaactc
     9301 gtcaaatata actaaagagt ctgctgttac gttttgtaat atagatacta tattcgtcat
     9361 atgagatgaa aatgttgata gactctgttc aatactctgt tcatctccta tatctgcaaa
     9421 tacattatca tatacacaca tactgcttcc ataatcaaca ggtatatgaa gtccactttg
     9481 tgtcataagt gcaaatagac ctacagtttt tattgtaaca gtcttaccac ctgtgtttgg
     9541 acctgttatc accaatgtgt ggaagtctct tcctaaataa attgtatttg ctactacctt
     9601 ctttttatcc aacagtggat gtcttccatt ttttatgttt aaatatttat cctcatttaa
     9661 agttggttct attcctctca tctgtataga aagttttccc tttgaaaatg caaagtccaa
     9721 tcttccaagt atctcttgat tagagattaa atcttcactc acttctccaa ccatagcaga
     9781 aagctctgat aaaattcttt ctatttcctc ttgctctcct agtttcaact gtctcaattc
     9841 attgttcatc tctactatag tcattggctc tataaaaagt gtcgcaccag aagatgactg
     9901 gtcatgtact ataccagcta cttgcgacct atattcagct tttacaggta ctacaaatct
     9961 atcacctctt agagaaatta ttgcatcttg taagtacttt tgataagtag ttgaacttat
    10021 tattgaattt aattttgacc taatggattg atttttttgg gctattcttc ttctgatatc
    10081 tctaagtgta cttgaagcat tatcagatat ttctacttca ctaattatag cattgtatat
    10141 ctggtcttct atgtccttgt atacatatag agaatttgat agagattgta ttattggata
    10201 attaaaatct tcttcatcac tactagataa gctatttctt aatcttctag ccactcttaa
    10261 ggtatctgct atcataatca gacttcctgg gtcaagagaa gcacctatat aagctctctt
    10321 taccttatct tctatatcat gaataccttc caaccctact gaacctctct ttataataat
    10381 tgattgagct tcactagttt cttctagcat agatttcact tctacaaaat cagtatttgg
    10441 tactaaattt tctatatatt ttagacctaa agaagatgag gctttcttct ttaataaatc
    10501 tattatttta ttatattcta gaactcttaa cgatttctca ttcattttct atattcacca
    10561 tcctcgatat gttaactttt ttatttaatt ttaaaaatgt atgttttcaa gcatttccaa
    10621 taaaaactgc aagttttatt tctttttcat ttctaaaatt attatatttt ttctctttta
    10681 gatatatttt acacatattt tgtattatac tatgtatgta gctatctata tattatacat
    10741 aaacatgctt tatttgtaat ataatctgtt aaaaaatctt ctttaatatt tttaaaggag
    10801 tttattttag cttataaaaa aaatagctac ttatatagat aaattaagga ggaacatgag
    10861 tcatgggaaa cgtattagaa ggattaaaac cagaaagtgt ttttaaaaac tttgagaaaa
    10921 tatctcaaat tcctagaggt tctggaaatg aaaaaggcat aagtgatttt ttacttagct
    10981 ttggtaaaaa tttaggtctt gaaactattc aggatgaaag cttaaacata ataataagaa
    11041 agcctgctac aaaaggatat gaaaattgtc caggtgtagt tcttcaagga catatggata
    11101 tggtttgcga aaaagaaaag aatgttgaac atgattttct taaagaccct ataaaattaa
    11161 gaattgatgg tgacatgata tatgctactg gaactactct aggagctgac aatggtatag
    11221 ctgttgctat gggtatggct atattagaag ataatacttt agagcatcct gcactagaag
    11281 ttttagttac tgttaatgaa gaagatggta tgaatggtgc agatgcacta gaccctagct
    11341 taattaaagg tcaatatatc ctaaatatgg actccgagga ggaaggatac ttattagtta
    11401 gttgtgctgg tggtaaaact tgtgttgtta gtttacctgt tgaatacaaa aaagttgaag
    11461 gtgacaaaca aggattatta gttgaggtta ctggactatt aggtggacac tctggtatgg
    11521 aaattgtttt acaaagagca aatgctaaca aagcaatagc tagagtatta agtgtattga
    11581 atgttgatta tgaattagct tctgttgatg gtggtactaa gcacaatgct atacctcgtg
    11641 aagctaagtg tgtaatagct gttaataaag ctgatgttga atctgctaaa aaacaaataa
    11701 ataacatttt aactgcattt aaacatgagt ttactacttc tgaccctggc atgacttact
    11761 caatagctga aacttctgta gacaaagttc ttactaaaga ttgtaaagaa aaagttgttc
    11821 aaatgtcttg cttaactcct catggagttc aatcagtaag tcttgatata gaaggattag
    11881 ttgaaagttc tactaacttc gctataattg aaactaaaga atctactatt gaatttttaa
    11941 cttctgttag aagttctgtt atgtctatta gagatgaaat cgctgataga ataagacttt
    12001 tggctcaagc tttaggtgct aattacgacc ttatagctca atacccagct tgggaattta
    12061 aaaaaggctc aaaattagaa aaaatatgca gtgaaactta tgagaaatta actggtaaag
    12121 ttcctactgt tatggctcta catgctggtc ttgagtgtgg attattactt gataaacttc
    12181 ctcatgctga agctatctct ataggaccag atatgtttga tgttcatact ccaaatgaac
    12241 atgttagtat cccttctgta gctaatgttt gggattatgt tattgaaata ttaaaatcta
    12301 tgaaccaata ttaaatttaa gttttgattc taaatagatt ttaattttaa gtaatctata
    12361 taaaattaac tctgtaaatt aatactacta ttagtataat atttactttc aatattaact
    12421 tatagaaata aagcctaatg ataggtaata atatgtatca ttaggcttta ttgtaattta
    12481 aatattatta aactttattc tgttttaaaa ttaattctta tactatttta aatctatttc
    12541 ttccttgtct cttagaatcg taaagagcat tatctgctat ttgatacagt tcatcatact
    12601 cttgaccacg ccttgatata gctaccccca tactaataga taaactttct ccatttggca
    12661 acttaatgaa atgaatatga ttgtatacat cattacactt attgataagc tcttccttat
    12721 ttattatatc attgatataa acactaaatt catctccgcc taaacgacct actatatcat
    12781 catttgcaga actttcatca agtgcctttg caacatcaat cagtacttta tctcctacca
    12841 ggtgaccata agtatcatta atcgacttaa aataatcaat atctataatc aataaagcac
    12901 catttttttt agttgataaa ttcaataaat tttgtgcagt taattttcta atatatgctg
    12961 cattataaat attggttaaa ctatcccttt gagccttatc taacaatcta ttcttttcct
    13021 ctttttcaga atcaatatta catattttcc cgattacata cattggtatt tttctatcat
    13081 tataaattat ttttgaagta attctaatcc agtgaaaagt atcatcaaac ataatagagt
    13141 acatttcagt tgttccattt tcttgtgatt ttattacatt aaaaaacttt ttattatatt
    13201 ctcttcttat tttatctatt tcatttgttg tattgccatt ttccccttcc ttatcatttt
    13261 tatctaaacc ataataggtt tcaatacccc tttctggcat agaaattatc aatttatctt
    13321 gcttataatc atattcaaaa aagtattcac ttgtcatttc atacaactga gtatgttttt
    13381 tgagttccaa agatatcttc tcgttcatac ttgtacgagt atgtaaacca aatgccataa
    13441 atccaataat acaaattaat aaactagcta caataaaaat agtctcaatt ggattctgtt
    13501 gcataatata aagcaacgag aaatcttgtc tatatgtagt attttggtag acaattgatt
    13561 gcattttttc tgatgaaata cttataatag ctttattaaa tatattcaat aattcttgtt
    13621 tatctttttt aattatacca aaacaaatac tctttgcttt ataagtttga ggaactagac
    13681 gcacattttt atacttaggt tgattcatat agtactgcat agaataacct tctccataag
    13741 tgtaatctgc atagccttta ttaacagctt ctatacactc ttctaaatta tcataccaaa
    13801 ctatattctc ttcaaactga ccatcatgat taataccttt ggttagagct atacgttttc
    13861 catctagact attctcattt aatttattat taatcatcat agtgtattga gtagaaataa
    13921 aaggtcgaga catagctaca ttatgttcac gagagtattc ataatcataa atcattcctg
    13981 ctacaatatc aatttcatgg tttttagtca tattttccaa atcttcttga gattttacag
    14041 gcacaagttc aaattccaat cctgttttat cacatatatg atttaacaaa tcaatagata
    14101 tacctcttaa ttcacctgta tctttatcta tattctgaaa tggtggtttg tttaaaagta
    14161 ctcctacctt taatttttct gtatctttaa tgtaattagt ttcttcatca gataaaaaca
    14221 tttgattatt ctctggtgta aagtatttct catataaagt agttgaaaaa taggggtcct
    14281 tctgttcaat attattgatt gctgagttaa gattttgagt taagtctgta ctacctttag
    14341 gagtaatgaa ataaaacggt cttggtgaga attttgctac agtacgtaca ccttctattt
    14401 gattcatact cacattaaga agtacatctg ctttaccatt ctttagtgct tctaattgct
    14461 cgtcatttgt tttacagtat accaattcag gtgacatcga attaatctta caatactcat
    14521 ctaactcctt aagcctagtt ttagaatttt ctattacagc aatacggatt tttttcacat
    14581 cttgtgaact cattatgtta tcaatatctg tattttcata taatgcttgt agtactgtat
    14641 ttgctactcc ataactattt cctgcataat tatatatttt ttgcattggc tcattgtata
    14701 acatacctcc cattatatca atttcaccat tttctaacat cttcattaat tttgtaatag
    14761 attcatttat ctctccagga acttgaacaa attcataatt ccatccagta tactgtgcaa
    14821 tttctagtaa atattcatat gtatacccat aatagttacc ttgctcatct atttcagtta
    14881 aacctttttg aataggataa gcaacacgaa tggtacgatt atcattacct atagcattga
    14941 tatcaaaaga aaatatgcaa gaagtgaata tagatgtaat tacaataatt aataaaagta
    15001 atttttttaa attcactaca caatcctcca tatagttaag atttacagta taaattactg
    15061 cataaacttg tagacatgaa aaaatagatt agtatataaa aggatatata aatctactta
    15121 gaaatttaaa tatccttaat atatacaata tatttaaatt ataactcatt tatatatcta
    15181 ttacaacaaa tttaccctgc taaataccta ctctatatcc cataatttta caagttgtag
    15241 ctaagtttaa gttctatctc atctcaaatt tagcagtgtt tctatttaca gttcttttat
    15301 atttcacatc tggataaccc aatataaatg aagtgataac cgcttcgttc tcgctcattc
    15361 ctaacagttc ttttatcttt ggctctacat ttgatgccat aacaaaaaaa ccattataac
    15421 aaattcctaa tccttgagaa taggccataa gttccatatt agatgcagcc aatcctccat
    15481 ctacataagc acttccacct agagatatat cacctacaac taccataagt agtggggcat
    15541 cataaaataa agaatcattt ccgttctctt tatatctttt gtacatcttc ttaaatgtat
    15601 ttttatacat agaacgtaca gggtcattgt catcagtatc taatgccaaa ttataaagac
    15661 cttctattgc tagttccttt acttctttta gtttttcttt tactaatatg tatcttatag
    15721 gctgtctatt cccacctgtt ggtgtgtatc taccagcttc cactatgttt tttatttttt
    15781 cttcttctac ctctatatct ttatattgtc ttatacttct tctaaattta ataaaattca
    15841 ataacttttc gggttctaat tcaaaacttt cacgattgta ttctattact tcatttttat
    15901 caaatccttg catttctatt gcatctgttg gacaaattgc catacagtga ccacagttaa
    15961 tacagagaat atttttaacc tttgctttat catccttaac ttttattgca tttacaagac
    16021 agtctgattc acacatccca cagcctacac atttttctaa atttatgtct atcattccaa
    16081 attattcctc cttttaaaaa atttacttta gtataaaaca aatagttgtc taagtcaatt
    16141 tttatttttt aacaatatac tagccttaac tacactttaa tggagaaaaa tatacataaa
    16201 ttagaactta ttcaaaataa aagataaaag aagcagatgt ctaaaagtaa actttttagt
    16261 taatgagaca ttctttattg tatgaaatca agtgtatatt tatcatttca acaataaaaa
    16321 aggcttatct ctattttgag ataagccctt ttctcaacta ctattgttta tcttaatatt
    16381 atgttaataa atttaatttg ctataacctt aagttacatt ttaatttgat ataactttaa
    16441 attacatttt taagtagtat ctttaaatta tattcttaaa atatatcatt aaactacatt
    16501 ttaatcaatc taaaaactat atcttaatta atcgaagaaa cttacattat aatcttttag
    16561 tttcccatta tcatatacaa tctttgcact aaagatattt ccagtcttag cccaatttaa
    16621 gaacaacatg tcaccttccc acatatttaa atcgaatatt ttatcattat caacccaaat
    16681 taaatttcct tctttacaat catttaaatc tttctcagat aattcacctt caaactcatc
    16741 tgagaaatac aaaaacatat accaatcttc ttcatcttta aattttggaa aagttatcaa
    16801 tcctttatat gacaatgatt taagagtaag accagtctct tcttttactt ccctaattac
    16861 acattcttct ggagtctctc cttgctcaaa ctttcctcca actcctacat acttaccttc
    16921 atgtatatca tctttctttt tatttctata caacatcaaa gtcttgtcat ctttttctat
    16981 ataacaaata gttgtaagtt tcatgtatct cgccttactt tctctttata ttctcaacta
    17041 attattttca gcactttcaa gcacatcact tatattcttc attgcattga gagaaagctc
    17101 tgcaaattca tcaaatggaa tccctatatc ttctatactt ctcattgcat ctctatttac
    17161 atttgaagca aatctcgttt ctttcattct tttaataaca gacttagtct ttacactggt
    17221 aagtttttta tctggataaa ctaaagctat agcagttata aacccagtta tagggtctgc
    17281 tgcatatatt gctttttcca ttaggctagt tcttgtaact cccattccat catgatgacc
    17341 aagtattgct ctatacattt cttcgtcacc gaagttttct tttttaagca gttctactgt
    17401 tgttgtagca tgtccttcac atatttctcc tggctttctt ccaactatat ctgaatccaa
    17461 atcatgtaaa agacctgcca ctgcccactc ttcttctttt tctggctcta gtcttttggc
    17521 taagtccctc ataacagctt caacagcata acaatgtttt ctcattctat cagtttctaa
    17581 atacttaaat aaaatttcta aagcttcttc tctcattttc actaatcccc ctgttttttc
    17641 atatatatga tatctttata tattaataca cataaattca agtttttata tatattcatg
    17701 tatatttttc atgttatatc tattctacac ctttatagta gtgtcctgca actgttccaa
    17761 tttcatctaa tttcttatac aagtctgtag catttttccc aagtttataa ttattactca
    17821 atacctcaat gtgagcttca ataacctttt taaccatctc actatcttca actgtaaaat
    17881 ctatcctaaa tacatctatt ccaatttcct ctaactcata tagattgtcc aacatacaag
    17941 taaccttcga attgtaaatt gtacttctac agaatatatc ttgtgttatc ctataacttg
    18001 cgtccgtaaa gtctctcaaa gcataattac tttccttaca tttagcaact ctcttatcct
    18061 ttttacaatc tctaacaact actcccatag gacagtattc tgtaaccatc ataggagcat
    18121 atccatatat taccgcttca acttctacat ctgtatattt taaagtttgt ttaatttcgt
    18181 ttaagtttag ttcttgtgac aaacaaactg tacttgctcc ctcttctttg aagtgattta
    18241 tactttcact attaaatgca tttaagtagc aatctatatt taagtctttt ttcttaaagt
    18301 attccataga gccccagtta ccaatttgaa ccttattaat tccttttata ttatccaata
    18361 tctcaaattg cttgtactct ttatttctaa caatccttgg tgctgaatag attaattttt
    18421 tgtcagtgac tgctgtatta gagtgattct tattcaaagt atctatttta cttgtatttg
    18481 ataaagtatc tatgttattt gtatttgaac ccattaattc tttaggaata cctaaagacg
    18541 aagaagtatc taaatccata gaaatacttg aatctgtaga aatatttgag accatttgta
    18601 aagcttttga taatgtgttt atatcttcat aatagattaa atccacatca taacttaata
    18661 ctgcttctaa ttgctccagg ttcttaactt taactctaat cttaggaact gtatcaagtt
    18721 ttttctgttt atttactcta tttggctggt attttaatct attcttctta aactttctat
    18781 cttttatctt tattctttct tcatctaaaa gctcaataca atctcttctc atttgattta
    18841 acacacttat aggcatactt acaccttcat ccaggtcaat ttctatctct tttaacttgt
    18901 agggtgtatt tccaagtttt tgaatttgtg tttctatttt ttctttactt aaagccacct
    18961 ttatagcttt ttcaactatc ttgtctcctt ctaccaccga tttatttcca caaaaatcac
    19021 ttataaaaag ctctggtttt ttacctagtt taatactcac ttttgcactt atattagttt
    19081 ttatgaactc tttatcttgc tgaaatgtag cttgtactct acccattaat tcgctatcag
    19141 aagtcttata tataatctga tttctctttg cttctcctac aaaatctaat tctatcaaat
    19201 cccctttatg tgctattgta gttatctctc cattctttat tatccttcca atagtacctc
    19261 cacctaagtt aattccatca ccttttttaa gcgtattttc taacattact ctaagtcttt
    19321 tgttctttct attataatct aaaacttttc caatatatag cccttgatta ttaggtctat
    19381 tagagttcat tatatcctta ccaacttcac ctaatatata tccttttgta aactttctat
    19441 taaatatggt atataaatta tttattattt cctcagaaat acttattttt ttattatcta
    19501 caaattcatt aatcgcttct ctatatcctg aaaccacagt agctacatac tctggctttt
    19561 tcattctccc ttcaattttt aaagataata cacctgcttc aattatttta tccaaatctt
    19621 ctatagtact taaatctcta ggactcaaca aataatctcc attacttttt actacttctc
    19681 ctgtatttat atctattaac tcatactttt gtctacatgg ctgtgcacac ctacctctat
    19741 ttccagacct attgcctatc atactactca taaggcattg acccgagtaa catacacata
    19801 aagctccatg tacaaaaact tctatatcta cagttgtatt ttcacaaatc cattttatct
    19861 catcaacatt taattctctt gctaaaacaa ctctatcaaa ccctacactc tgaaggtatt
    19921 ttacatcttc taaggaatgt gctaccattt gagtgcttgc atgtagttca aagtcaggta
    19981 atagtttttt tataagtctt gccattccta tatcctgtac tattaaagca tcaatatcta
    20041 tatcgtataa aaattttaca tactctacaa aatcttctat ttcatactgt tttatcaatg
    20101 tatttacagt aacaaagact tgcactcccc ttatatgaga gtatttaaca gcctcaatca
    20161 gctcattttt atcaaagtta tttgcagatg ctctggcact aaagtcttta ccacctaaat
    20221 acacagcatt tgctccgttt tgaactgctg cttttaatga atcaaaagag cctactggtg
    20281 caagtaattc tatttttttc atatttttct cctctattta tacttgttta ggttaatata
    20341 attatatcta taacttaaat atttatatta aatttatata ttgattacta tattttaaat
    20401 tcttcaatta atttatatac tgcttactaa atttaaaaca ttttaattaa tttgtacact
    20461 gtttactata ttttaaatgc ttcgattaat ctatatattg cctactatac ttaagacatt
    20521 ttaattaatt tatacactgc ttactatact taaaatactt cagttaatct atatattgcc
    20581 tactatactt aagatatttt aattaatttg tacactgttt actatacttg aaatgcttca
    20641 gttaatctat atattaccta ctatacttaa gacattttaa ttaatttgta cactgcttac
    20701 tatacttaaa atacttctat taacttatat attgcttact atattttaaa ttctttaatt
    20761 aatttatata atatttgcta tatataaagt atatcaaata aaagcctcat ttttttgtaa
    20821 atttacagaa agttaatttt cattaaatga atcatcttta ttgataaaca taattccaaa
    20881 ggccactgcc aaaataaaaa aacatattag tattgatttt tccacgaaaa tttttgtaaa
    20941 aaaagttcct aaaatagctc cagatataaa gaatataata atagcataat actgtaaact
    21001 cttttttatc ataaaaatat tcttagtatg tatataatga aataaagttt ctgtagcact
    21061 tcttaaattt ccagtacaca ttgtcgttgc aaaactattc ccatttactt ttctaaaact
    21121 ctctacttgc attgaacaaa taaaagaaat agcaacattt acaaacatat taaatcttcc
    21181 tctaggaaca aatgaaacaa taagtattgt aatcatttca attataatga taacctgacg
    21241 ccaatgaatc tttgtcttat tatttgtaaa atactttctt atcttctcgg ctataaatac
    21301 acctaacata aatgccaaaa taggaatcaa ataataaaat gattccttta cattaccttc
    21361 tgctatatta agcccaaata aaactatatt tccagtttgt gcattagcaa agactttacc
    21421 tcttaccaca taagtataaa tatccaaaaa tcctcctgtt attgccaaca aagaacataa
    21481 aaagaatgat tcagacatct gactcttgct tgtcatatat aaacctctca ctttctaatg
    21541 atagatacaa aatcaaaatc attttaaatt ctatttttca ttgatataaa aaaattattt
    21601 atatttaaat attgtctata tttatacatc atttatattt aaacattatc tatatttata
    21661 tcatttatat ttatttatat ttaaacatta tctatatcta tatatca
//
LOCUS       DAEPXF010000058        21508 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain HB27
            SAMN13376389-rid12479423.denovo.062, whole genome shotgun sequence.
ACCESSION   DAEPXF010000058 DAEPXF010000000
VERSION     DAEPXF010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13376389
            Sequence Read Archive: SRR10518432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 21508)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21508)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001069624.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001069624.1
            Genome Coverage       :: 315x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/15/2021 01:54:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,165
            CDSs (total)                      :: 4,076
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 89
            rRNAs                             :: 10, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 10 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 19 of 69
            Pseudo Genes (incomplete)         :: 44 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 10 of 69
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21508
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13376389-rid12479423.denovo.062"
                     /strain="HB27"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="China: Hebei"
                     /collection_date="2017"
     gene            284..3187
                     /locus_tag="KRL67_001831"
     CDS             284..3187
                     /locus_tag="KRL67_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBH1522711.1"
                     /translation="MELRDKLVNIINTENKKDPLTDVQIAKFLSTTRENITNLRKELN
                     ISNSRQRRYPYLKSAISAILQKNKNISISEITRELMTEGFNISRRVVEELLPKESLEM
                     DVVEESEEESNDPFETLIGNKGSLRNAVEQAKSAILYPPKGLPTLIIGESGVGKSLFS
                     RHMYEFAKQKKIVKESSNFVVFNCADYSDNPQLLLSLLFGYKKGAFTGAEYDTPGLVE
                     EADEGVLFLDEIHRLPPKGQEILFSILDRGKFRRLGETNAERKVSIMLIGATTENVET
                     NLLLTFRRRILMLITLPPLHDRLLKEKIDLIYNIFQQECNRINAKIFVDKNVIEILAL
                     KKFSGNIGQLQNMIQVLCARAFMKFINSKDEDSESIVVVDINEVLKLKDSFKDAAFQE
                     IEYTEIRKYLKNAIFIPFNLEESSVKGNLLSDEYILPEDIYKTIEKKYYDLKSLDIED
                     IDIENILWTFILNRFADLKFNLDSNEDIFSMSDLNSFVSDSLVKLVKKLMNDIVEKNM
                     NLEVNKNMFKYLAIHLEEAIKRIKLNQKIINVNLEKIKIDFSKEYEISKSFAIKIEES
                     IGIKIPDDEIGFITLYIKSALKNEVKKDKVGLIIISHGRIATETVNVVKELLGVKFPV
                     AIDMPLDEKPINIYNKAVELSKIIDQGKGILFLVDIGSLTNIGQIVNKRTNINTKTID
                     RVDLLMALEATRKVSMGEEELDEIFFSLLKDRMGYNYELEKHIDKSNAIVTLCLTGEG
                     TAKYISKTLEEKYDNTKCYQMSALDENLFCKIENLKETNNILAIVGTINPKIPGINFI
                     PYNKELFKNLDIYLSKSKQQDDRPVKSYERMIDEDLVIFEPDIYFKKDLLEYVCSILI
                     NKEYVEKDYLDSVLHREEMLPTYSKGAIGVPHGDSSTVNSTRFVFVKLKNPIDWGVGN
                     VNFIFMPVFQANDKEIVKNVLDILKDAEFMNNANKCFDKDAFRRIIFDKFKHL"
     gene            3220..3666
                     /locus_tag="KRL67_001832"
     CDS             3220..3666
                     /locus_tag="KRL67_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBH1522712.1"
                     /translation="MNVDLISLGLESNDYESVIEELGSVMCKKEYVKDTYINAVLERE
                     RTLPTGLDIGEMCVAIPHTDSKHVNESNVAVGILKNPVKFNSMIDPKDRLDVELVFLL
                     AVKNPDSQVKLLKDLMSVFQNIKLLKNIKNASTKEEVAKLLDFIEI"
     gene            3789..4091
                     /locus_tag="KRL67_001833"
     CDS             3789..4091
                     /locus_tag="KRL67_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBH1522713.1"
                     /translation="MKQFNVLSVCGSGTVTSSMVAGKLKEVLGNKGISITTTEARPTE
                     ALNLAQSGRFDFITFTSPLQTGDYGIPTINAFACLTGIGEETFFEEVLTVIEGIDK"
     gene            4272..5678
                     /locus_tag="KRL67_001834"
     CDS             4272..5678
                     /locus_tag="KRL67_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HBH1522714.1"
                     /translation="MLEFMKSIIDTFGSAIIVPIIIFIIAKIFKVTTKKSFLSAVYAG
                     VALQGFTLILNSFTPIITPVINRMVESTGVNLPVFDVGWQATSLVAFSTSAGMIYLGL
                     GILLQTILFLIKWTDVFQPSDLWNNYSYMVWGAMVIGVTGNFPLGIACMVLLNLYSLL
                     ISELVAKRWSSYYRYPNCTIIAMHNVEASVFAVFADPIYNKLGLNKIKLNPKELEKKL
                     GFLGEPITLGLFLGMFIGILGNMTRINTMEAWGEIMKVGISTSAVMAIFPKVASMFAQ
                     AFAPITEAARKIMQKAGNREWYIAVNDAVGYGEPATLISGLILIPIMLVIAMVLPGNK
                     VLPVVDLLAIPYMVQGLVAIHNGNIPKVLVSGIIWFGLGLYVCTSTAPLFTDMATNIG
                     VAIPAGAMLITSFNILGKPLMGLVFFAFLSANPIYIGLAVVIYFVLWALFRKNKTSIL
                     DYLEKQALKNVEEEPVAV"
     gene            6022..7080
                     /locus_tag="KRL67_001835"
     CDS             6022..7080
                     /locus_tag="KRL67_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012101107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HBH1522715.1"
                     /translation="MGNKMRASVLYNVGDVRYEMVDIPEITDTQVLVNVKYVGICGSD
                     LPRSMVSGLSGNTKYPLILGHEFSGEVVKIGEKVKHINVGDRVAVAPLVPCGKCDYCN
                     EGNFGLCDDYNIIGTRVNGAFAEYVRVPEEHILKLPDTLDYETAAGIEPSTIAYHGIS
                     KSNIRVGDSVVVLGCGPIGQFAIQWAKVFGASKIIAVDIFDEKLELSKLLGANYILNS
                     KEVNVIKEIRKITNGGADVVIETAGSRFTQEQSLFVAKKRGNIVFVGISHTELPLSAD
                     ATECILRGELTLKGSWNSYTSPYPGRAWTATLDFMGKGAIIFKPMISHKIGLNEVGDF
                     LSKMSKREINFNKILVEI"
     gene            7182..7868
                     /gene="araD"
                     /locus_tag="KRL67_001836"
     CDS             7182..7868
                     /gene="araD"
                     /locus_tag="KRL67_001836"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HBH1522716.1"
                     /translation="MLENLKEEVLNANLELPKKKLITYTWGNVSGIDREKGLVVIKPS
                     GVEYSNMTVEDMVVVDLEGNVVDGKLRPSSDTPTHLVLYKEFEEVGGIVHTHSSWATI
                     WAQIGKSIPSCGTTHADYFYGSIPCTRKMTREEICNEYEKETGNVIIEEFMGRNPIHC
                     PGIIVNDHGPFTWGKDANEAVHNAVVLEEVAKMAYYTELMSPDNIMDKVLMNKHFSRK
                     HGKNAYYGQK"
     gene            7886..8533
                     /locus_tag="KRL67_001837"
     CDS             7886..8533
                     /locus_tag="KRL67_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HBH1522717.1"
                     /translation="MLESLKGKVVEIAIKAEHYGLCKEKTGNFSIRDKDTGYILITPS
                     GIGREELKVEHICVLDLNGNVIEVEKGIKPSSEVLMHIEIYKTRENINAISHTHSLYA
                     TAFSVAKKPIIPIVYESVNYGGYVDIAPYERPGTLKLAKSVSKLLIDTDACLLERHGL
                     TTVGNDLNEALLKSRYVEEVAEIYYKSLVLNQFKELEVVDVDEFKSWKYPEEIKF"
     gene            complement(8706..10073)
                     /locus_tag="KRL67_001838"
     CDS             complement(8706..10073)
                     /locus_tag="KRL67_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:alanine symporter family protein"
                     /protein_id="HBH1522718.1"
                     /translation="MNLIDILNKVDAFIWGPPLLVLLVGTGILLTVKLGVVQITKLPR
                     ALKLIFSAENKGSGDVSSFAALCTALAATVGTGNIVGVATAIKAGGPGALFWMWIAAF
                     FGMATKYSEGVLAIKYRAKDKNGQVSGGPMYYIVNGMGEKWRPLAIFFAISGILVALL
                     GIGTFTQVNSITDAINNSFGIDPRITGVVLAVFVALVVFGGLKSISNVATKIVPFMAV
                     IYVVICGIILISFWNKIPETFMLIIKSAFTPTAATGGFLGATMSLAIRNGIARGVFSN
                     ESGLGSAPIAAAAAKTEWPAEQGLISMTGTFIDTIIICTLTGFSLVISGVWCSDLNGA
                     VMTQAAFNGAIPTFGPILLTISLTLFAFTTILGWSYYGERCFEFLFGVKGMNGYRTVF
                     VAMVLLGAFLKLEVVWIIADIVNGLMAIPNLIALLALSPIIVSETKKYFEHINSPENQ
                     IKKNA"
     regulatory      complement(10262..10350)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00504"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="glycine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="glycine"
                     /db_xref="RFAM:RF00504"
     gene            10778..12898
                     /locus_tag="KRL67_001839"
     CDS             10778..12898
                     /locus_tag="KRL67_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HBH1522719.1"
                     /translation="MGKILVLAEKPSVGRDIAKVLGSKNEKNGYIEGPKYVITWALGH
                     LVTLADPESYGERYKSWSLEDLPILPKYLKTVVIKKSGKQFNTVKSQMNRNDIDEIVI
                     ATDAGREGELVARWIIEKSQVKKPIKRLWISSSTDKAIKDGFAKLRSGKEYENLYYSA
                     IARAEADWIIGINATRALTTKYNAQLSCGRVQTPTLAMLLKREEEIRNFKPKEYYGLE
                     LIATKGNSDIKFIWNDKNNNSSTFSKEKIESTLKKVKGVDIKISEVNKSYKKKYSPAL
                     YDLTELQRDANKMFGFSAKETLSIMQKLYEHHKVLTYPRTDSRYLTSDIVGTLKDRIK
                     AVNISDYSRICNKLLKSNITANKSFVDNSKVTDHHAIIPTEQKVFMSDFSDKERKIYN
                     LVVKRFLAVLSPPFEYEQTTISATVEGEIFKAKGNKVINLGWKESYKDIEDEDEYESI
                     PNVLEKDILKVKGLKITSGKTNPPPYLNEGTLLTAMEKNSLGTVATRADIIEKLFNSF
                     LMEKKNKDIYITSKGRQLLDLVPVDLKTPELTASWEKKLLDISKGKLSKNTFINDMKD
                     YSKDIVLEVKNSDNKFKHDNLTKNRCPECGKFMLEVNGKKGKMLICEDRECNTRKTIS
                     QVTNSRCPNCHKKLELRGEGEGRIFTCSCGYREKLSSFNKRKQEEKQKASKKDVNQYL
                     KNQNKNQETFNNPFAEALAKLKNK"
     gene            13002..14255
                     /locus_tag="KRL67_001840"
     CDS             13002..14255
                     /locus_tag="KRL67_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FliS"
                     /protein_id="HBH1522720.1"
                     /translation="MDLSGIFKYYCKECENTWNNSSVELFENIETYSKDSQKKREKEL
                     DKLMNTISVHLERYPSDAVLRKMWVKKGEVFLQKTLEKENIFKLEKMDVEDRKKFLDI
                     TKQFIRDARKFDSDLPIGDIMQAMRNVWISNALQLLFGKEVYYSKANFAYSMLYPYTD
                     NYLDNTSIDKNDKILFNNWLEKRLLGEHIKSKDYHESKVSQMIDYIESVYPREKFTEV
                     YESLLLIFKSQVNSLKQHGKENHLCKEDLLSISIEKGGSSVLVDGYLISGLMTKEEIE
                     FCIGYGFLLQISDDLQDIKEDLKYNHKTIITEMAKGGTLDKVVNKLINFTIELIDSFK
                     INNKNKSVITMIKNDCLMLILFSVVYNAEFFSVGYIKEVEKFIPYTIDYSLEIEEKIK
                     EKFKNIDVLNNENEYKEMIDIICSE"
     gene            complement(14409..15470)
                     /gene="ytvI"
                     /locus_tag="KRL67_001841"
     CDS             complement(14409..15470)
                     /gene="ytvI"
                     /locus_tag="KRL67_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation integral membrane protein YtvI"
                     /protein_id="HBH1522721.1"
                     /translation="MDIQKQKNFIIKFTYVVLITSIVYIVLKFLLPLLMPFVISFIIA
                     SILRPIIRLITNNTNLNRTLISIVILLVFYGLCIFLLVSFGVKIFASVSDVFFRLPEI
                     YKSSIQPTLNTLFSKIDASTPNVNLALILGWDNISQSMMSLVASISTNALNAIASIAS
                     KTPAFMLKLIITLIASFFFTFDYQKIVNFILKQFPEKSQFMIINIKNSSINALLKLLK
                     AYAILLSVTFIELLIGLTILKVENAFTISIIIALVDILPVLGTGSILTPWMIISLING
                     NINLAIGLLILYIIITVVRQILEPKVVGHQIGLYPLITLMCMFVGAQLFGIAGLFGFP
                     IAATIIKNLHDNGIITAFK"
     gene            15744..16484
                     /locus_tag="KRL67_001842"
     CDS             15744..16484
                     /locus_tag="KRL67_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein phosphatase"
                     /protein_id="HBH1522722.1"
                     /translation="MKNFRDLGGNKTEDGRTVKKGLFYRSAKLSNLSENDIKILKELN
                     IKYIFDYRSDEEARKHPSTIISNIKNIRIPAMRELEESGGSFGSIEDMIDGLFEKDGA
                     FNMLNNSYYNLPINNPSYKKLVELIRDYSNLPILNHCTAGKDRTGVGSAIILMILGVS
                     RENIMKDYLKSNDFADKEIERFIEYKPKFKDIPKENLKYIFGVNEEYMKTAFRRIDEE
                     YTSVEAYLYGEFNLNKEEIRKLRNQYLE"
     gene            16496..18274
                     /locus_tag="KRL67_001843"
     CDS             16496..18274
                     /locus_tag="KRL67_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HBH1522723.1"
                     /translation="MSLNITARQINIIKILLNSKDTISGLALSQEIGCSSKTIQNEIK
                     DINKQLRNGKILSIRGVGYKLEGSFDEVNLNSNLYDDVDRVEYIIRKILTLSNEEKNT
                     IKLEDLADSMYVSVSTVKNDLKEVKRMLEKYSISVKSKHKQGICVLESEDKILNCIVD
                     LSNKRDNQLSLNDFLNDDVKEKKLLIKKLLLDILNENRLVLTDIEFKSVLNNILVMLS
                     RHKYNEKEFIENYIENYRSKRSSILENEQNKEVIINSIKTFCKNLKLATSIDISNDKV
                     FEEFLYKHISSLCKKIKLGINQSPIMAQDIKIKYPFAFELANIAKKTIEKDLKIEINE
                     DEVANIALHIGGAVERASYNNKDKVFKTIIVCTSGIGTSMLIKAKLENIFKEKLEILK
                     IIPSYLVEYIKVIDVDFVISTVPIEVGDVPVINISPILSEKEIKLIEKYIETGNIYLD
                     LDIKSLFDSELFFTDLVFKRKEEVIDFMASKLVQKGYIDEDMKKSYFEREKIATTEIG
                     NMVTIPHGAIGKIFDNKIAIGILKKPISWEISDVRLIIMLALDKDKILDYEVIFSKIY
                     KRVDSIAKVISICENSSFEKFVNLFN"
     gene            18441..19358
                     /gene="pfkB"
                     /locus_tag="KRL67_001844"
     CDS             18441..19358
                     /gene="pfkB"
                     /locus_tag="KRL67_001844"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HBH1522724.1"
                     /translation="MIYTVTLNPSIDYIVKLDELKTGSTNRVNEEYVYPGGKGINVSR
                     ILKELGNDNTSLGFISGFTGEYIIRTLEEKKLKTDFIKIKSGFSRINVKIKALKETEI
                     NGQGPNIDDEDIDILYKKLDKLNQDDILILAGSIPSTLDEKLYENIMARLEKKNIKVV
                     VDATKNLLLNVLKYKPFLIKPNNNELEELFGVKLNSIEDMVKYAKRLKEMGAINVLVS
                     MGKDGALLITEEDEVLISDVPKGKVKNSVGAGDSMVAGFISGYLNTGKYDYALKLGAA
                     SGSATAFSYDLAKREYIDKLVNEISVKQF"
     gene            19395..21314
                     /locus_tag="KRL67_001845"
     CDS             19395..21314
                     /locus_tag="KRL67_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBH1522725.1"
                     /translation="MKIIDLLDEKSIKLNLNSKTKQEAIEELVDLVANSGNLNDKENY
                     KKAILAREEMSTTGIGEGVAIPHAKNSSVTKACIAAAVSKEGIDYESFDGSLSNLFFM
                     IAAPDGANNTHLEVLSRLSTILMDEDFRNKLINSSSEKEFKEIIDKKEREKFSEEYQD
                     EKVAEKNIIDTKENDANKYPKVLAVTACPTGIAHTFMAAESLNKMAENKGVSIKVETN
                     GSAGVKNKLTKDEIENATCIIVAADKNVDMARFNGKKVIKTKVADGIHKAEELIDKAV
                     NGDAPIYHGGDGSHNESNEESESGFRKVYKHLMNGVSNMLPFVIGGGILIAIAFLLDD
                     YAINPSNFGSNTPIAAFFKGIGDKAFGFMLPVLAGYIAYSISDRPAFVVGFVGGALAG
                     DGGSGFLGALLAGFIAGYLVEGLKKIFSVLPASLEGIKPVLLYPLLGTLLMGIIMTFL
                     VIPPVTAINNAMIGFLNGLGGTSKIVLGLVLGGMMAVDMGGPVNKAAYVFGVASLESG
                     QFEIMAAVMAGGMVPPLAIALATTFFKNRFTKEERDSGKVNYVMGLSFVTEGAIPFAA
                     GDPLHVIPACVGGSAVAGALSMLFNAALRAPHGGVFVIPVVTHPFAYILAIAVGALVG
                     MMLLALLKKPLNQEV"
BASE COUNT         8626 a         2316 c         3774 g         6792 t
ORIGIN      
        1 attgttgagt agatacgtac tattatatat gattgagcaa gtttttcaat cataaattta
       61 taaagtattt tgcattacaa tttaaacaaa gagttaaatt atttaagatt atgaataact
      121 aatacattta gtaaattact aaatatacaa aatgtaaata cttatcaaaa tgtataaata
      181 cttatgacat ctagttactt acagggtttg agtagaaaaa aatatcaaag taagtatggc
      241 atgatagttg cttatatatt tatcaagaaa ggagttatta agcatggagt tgagagataa
      301 acttgtaaac atcataaata cagaaaataa aaaagatcca ttaacagatg ttcaaatagc
      361 aaagttttta agtacaacta gagagaacat aactaatttg agaaaagaac ttaatataag
      421 taattcaagg caaagaagat atccttattt aaaatcagca atttcggcaa tattacaaaa
      481 gaataagaat atttctatat cagaaattac aagggaattg atgacagaag gctttaatat
      541 atctagaaga gttgtggagg aacttttacc taaagaaagt ttagaaatgg atgtagtaga
      601 ggaatctgaa gaagaatcga atgacccatt tgaaactctg ataggtaata aagggagtct
      661 tagaaatgct gtggagcagg ctaaatctgc tatattgtat ccacccaaag ggttaccaac
      721 gttgattatt ggggaaagtg gagttggtaa gagtttattt tctagacata tgtatgagtt
      781 tgcaaagcaa aaaaagatag taaaagaaag ctccaatttt gttgtattta actgtgctga
      841 ttatagtgac aatccacaat tattattatc tttgttattt ggatataaaa aaggtgcatt
      901 tacaggtgct gaatatgata caccaggtct tgtagaagaa gctgatgaag gagttttatt
      961 tttggatgaa atacatagat tgcctccaaa aggtcaagaa atactatttt ctatcttaga
     1021 taggggaaag tttaggcgat taggtgaaac taatgctgaa agaaaagtaa gtattatgtt
     1081 gattggggca actactgaaa atgtagagac taatttatta ttaacattca gaagaaggat
     1141 actaatgcta ataactttgc caccattaca tgatagatta ctaaaagaga agatagacct
     1201 tatatacaat atctttcaac aagagtgtaa tagaataaac gcaaaaatat ttgtagataa
     1261 aaatgtgatt gaaatacttg cactaaaaaa gtttagtgga aatataggac aattacaaaa
     1321 tatgattcag gttctttgtg cgagagcttt tatgaagttt ataaattcca aagatgaaga
     1381 tagtgaatcg atagtagtag tagatataaa tgaagtttta aagcttaaag attcttttaa
     1441 agatgctgcc tttcaagaaa ttgaatatac agagataaga aaatatttga aaaatgctat
     1501 atttatacca tttaatttag aagaatcatc agtaaaagga aatcttttaa gtgatgaata
     1561 tatattacca gaggacatat acaaaacaat tgaaaagaaa tattatgacc taaaatccct
     1621 agacattgaa gatatagata tagagaatat tttatggact tttattttaa atagatttgc
     1681 tgacttaaag tttaatctcg atagtaatga agatattttt tcaatgagtg atttaaatag
     1741 ttttgtaagt gatagtttag tcaaactggt aaaaaaactt atgaatgata tagttgaaaa
     1801 aaatatgaat ttagaagtaa ataaaaatat gtttaaatat ctagcaatac acttagaaga
     1861 agctataaaa agaattaagc taaatcaaaa gataataaat gtaaatttag aaaaaataaa
     1921 gattgacttt tcaaaagagt atgaaattag taaaagtttt gcaataaaaa ttgaggaatc
     1981 aattggtatt aagattccag atgatgaaat tggatttata acgctctaca taaagtctgc
     2041 tctaaaaaat gaagtgaaaa aagataaagt agggttaata ataatttctc atggacgtat
     2101 agctacagag actgtaaatg tagttaaaga acttttaggt gtaaagtttc ctgtggctat
     2161 agatatgcct ttagatgaaa aaccaataaa tatatacaat aaggcagtag aattgtcaaa
     2221 aataatagac caagggaaag gcatattatt tttggtggat attggttcac ttacaaacat
     2281 aggtcaaata gtaaataaga gaacgaatat aaacactaaa actattgata gagtagacct
     2341 cttaatggca ctagaagcaa ctagaaaagt ttctatggga gaggaagaac ttgatgaaat
     2401 atttttttct ttgctaaaag ataggatggg atataactat gaattagaaa aacacataga
     2461 taaatcaaat gcaattgtaa cattgtgtct tactggagaa ggaactgcta agtatataag
     2521 taaaacattg gaagaaaagt atgataacac taaatgctat caaatgagtg cattggatga
     2581 aaatctattt tgtaaaattg aaaatctaaa ggagacaaac aacatattag ctattgttgg
     2641 gacaataaat cctaagatac caggtataaa ttttataccc tataataaag aacttttcaa
     2701 aaatttggat atctatctat caaaatcaaa acaacaagat gacagaccag ttaaatcgta
     2761 tgaaagaatg attgatgagg atttagtgat ttttgaacca gatatatatt ttaaaaagga
     2821 tttattagaa tatgtatgtt caattcttat aaataaagaa tatgtagaaa aagattactt
     2881 agattctgtt ttacatagag aagaaatgct tccaacatat tctaaaggag ctataggtgt
     2941 gcctcatgga gattcttcta ctgtaaatag tacaaggttt gtttttgtta aactaaagaa
     3001 tcctattgat tggggtgtag gtaatgtaaa tttcatattt atgccagttt ttcaggctaa
     3061 tgataaagag attgttaaaa atgttcttga tatactaaaa gatgcagaat ttatgaacaa
     3121 tgccaataag tgttttgata aagatgcttt tagaaggatt atatttgata aatttaaaca
     3181 tctgtagcca tagcaaaaaa attgagagga gttagagtaa tgaatgtaga tttaatatca
     3241 ttaggtttag aatctaatga ttatgaaagt gttattgaag aattaggaag tgtaatgtgt
     3301 aaaaaagaat atgtaaaaga cacatacata aatgctgtct tggaaagaga aaggacatta
     3361 ccaacaggtc tagatattgg agaaatgtgt gttgctattc cacatacaga ttcaaagcat
     3421 gtaaatgagt ctaatgtagc tgtagggata ttgaaaaatc ctgtaaagtt taattcaatg
     3481 atagacccaa aagatagact tgatgtagaa cttgtatttt tattagcagt aaaaaaccca
     3541 gattcacaag taaaactgtt aaaagattta atgtcagttt ttcaaaatat aaagttatta
     3601 aaaaatataa aaaacgcatc aacaaaagaa gaagtagcta aattattgga ctttatagaa
     3661 atttaatgta ggagaatttt ataaataaaa aattaaaatt taaacaatca aagtaaatat
     3721 tattttttta ataggtttaa tcttatataa aataaaaatt tagagtgaaa aattaggagg
     3781 aaattattat gaaacaattt aatgtattat cagtttgtgg ttcagggaca gttacatcat
     3841 ctatggtggc aggtaaatta aaagaagtat taggtaataa ggggatttct attactacaa
     3901 cagaagctag accaacagaa gcactaaatt tagcacaatc aggaagattt gactttataa
     3961 catttacgag tccacttcaa acaggcgatt atggaatacc aactataaat gcatttgctt
     4021 gcctaactgg tataggggaa gagacattct ttgaagaagt tttgactgtt attgaaggga
     4081 tagataagta agttttggag atgtgaattt atagtattta aatcaatgat ttagatacta
     4141 taataagcta atattaacta aatatttgtg gaaaaaacat tttatatgtt gtcataaatt
     4201 aaaaaattat aaaaatagtc acttaagtat taaattaagc ttataaatat ttataagtag
     4261 gagggaaaaa gatgttagaa tttatgaaat ctataatcga tacatttggt tctgcaatta
     4321 tagttccaat tataatattc ataatagcaa agatatttaa agtaactact aagaaatcgt
     4381 ttctttctgc tgtgtatgca ggagttgctt tacaaggttt tacactgatt ttaaattcat
     4441 ttacaccaat aataacacca gttattaata ggatggttga gagtacagga gtaaacctac
     4501 cagtttttga tgttggttgg caagcaacat cattggttgc attttcaaca agtgcaggta
     4561 tgatatattt aggtcttggt atattattac aaacaatttt atttttgata aaatggacag
     4621 acgtgtttca accatcagat ttatggaata actattctta tatggtatgg ggagcaatgg
     4681 ttataggagt aacaggaaat tttcctttag gtatagcatg tatggtttta ttaaacttat
     4741 atagtttatt aatatcagag ttagttgcaa agagatggtc cagttactat agatatccta
     4801 attgtacgat aatagctatg cataatgtcg aggcttctgt gtttgcagta tttgcagacc
     4861 ctatatacaa taaattggga ttaaataaga taaaattaaa tccaaaagaa ttagagaaga
     4921 aattagggtt tttaggtgaa ccaattactt taggtctctt tttaggcatg tttataggta
     4981 tattaggcaa tatgacaaga ataaatacta tggaagcttg gggagaaata atgaaagtag
     5041 gtatatctac aagtgcagtt atggcaatct tccctaaagt agcaagcatg tttgctcaag
     5101 catttgcacc tataacagaa gcagctagaa agattatgca aaaagctgga aaccgtgagt
     5161 ggtacatagc agtaaatgat gctgttggat atggtgaacc tgcaacttta atatcaggtc
     5221 ttatattaat tccaataatg cttgtaatag ctatggtact tcctggtaac aaggtattac
     5281 cagttgtgga tttattggca atcccatata tggttcaagg tttggttgcc atacataatg
     5341 gtaatatacc aaaagtatta gtttcaggta taatttggtt tggattagga ttatatgttt
     5401 gtacatctac agcaccatta tttacagata tggctacaaa tataggtgta gctatacctg
     5461 caggagcaat gttaataaca agttttaata tactaggaaa acctttgatg ggattagttt
     5521 tctttgcatt tctaagtgca aatccaattt atataggtct tgcagttgta atttactttg
     5581 ttctatgggc attgtttaga aaaaacaaaa caagtattct tgattattta gaaaaacaag
     5641 cacttaaaaa tgtagaagaa gagcctgtag cagtttaagt ttataaaaat gaaataagaa
     5701 taattaaagg agtttataat tgattttata taagtatcag ataatacctg agaaatattt
     5761 aaatgaaata tagacttctt tttgtttgat aatatttgta attataatac attgtatttt
     5821 tatagttgag ttttctgtat aacctgccat ttaaaacata ctcttttata tttatacagt
     5881 aatgtggttt aattttataa aatgtaatcc ctactggtaa aagactttgt atataaggca
     5941 tttgattgaa taaaccattt atcttttgta tggcatagat attgctatat agaagtatat
     6001 agcatgaagg gagatattta tatgggaaat aaaatgagag catcagtatt gtataatgta
     6061 ggtgatgtca gatatgaaat ggtagatata ccagaaataa cagatacaca agtgcttgta
     6121 aatgtaaaat atgttgggat atgtggttca gatttaccta ggtctatggt aagtggtttg
     6181 agtggaaata caaaatatcc attgatttta gggcatgaat tttctggtga agttgttaaa
     6241 attggagaaa aagtaaagca tattaatgta ggagatagag tagctgttgc accattagta
     6301 ccttgtggaa aatgtgatta ttgtaatgaa ggtaattttg gcttgtgtga tgattacaat
     6361 ataattggaa caagagtaaa tggagcgttt gcagaatatg taagagttcc agaagaacat
     6421 attttaaaac taccagatac attggattat gaaactgctg ccggaattga accatcaact
     6481 atagcttatc atgggatttc aaagtctaat attagagtag gagatagtgt agttgtgtta
     6541 ggatgtggtc caataggtca gtttgctata caatgggcaa aggtgtttgg tgcatctaag
     6601 attatagcag ttgatatttt tgatgaaaaa ttagaacttt ctaaacttct aggagcaaac
     6661 tatatactta actcaaaaga agtaaatgta atcaaagaaa taagaaaaat aacaaatggt
     6721 ggtgctgatg ttgtaataga gactgctggt agccgattta cacaagaaca atctctattt
     6781 gtagcaaaaa agagaggaaa tatagtgttt gtaggtatat cacacacaga attaccactt
     6841 agtgcagacg ctactgagtg tatattacgt ggagaactta ccttgaaagg ttcttggaat
     6901 tcatatacaa gcccttatcc tggaagagca tggactgcaa cattggattt tatgggaaaa
     6961 ggtgccatta tttttaaacc catgatttca cataaaatag gtcttaatga agttggtgac
     7021 tttttaagta aaatgagcaa gagagaaatt aatttcaata aaatcctagt agaaatataa
     7081 gaagaattta ataaaaaatt ctaaaagcaa ttaaatttta taactatgac attaaataaa
     7141 ataaaaataa ttttcttaaa taaaatatag gaggaagaaa agtgttagaa aatttaaaag
     7201 aagaagtatt aaatgcaaat ttagaattac ctaagaaaaa gctaattact tatacttggg
     7261 gaaatgtaag tgggatagat agagaaaaag gattagtggt gataaaacca agtggtgtag
     7321 agtatagtaa tatgactgta gaagatatgg ttgtagttga tttagaggga aatgtagttg
     7381 atggcaaatt aagaccttct tcagatactc cgactcatct agttttatat aaagagtttg
     7441 aagaagtggg gggaatagta catacacatt caagttgggc aacaatttgg gctcaaatag
     7501 gaaagtctat accatcttgt ggtactactc atgcagatta tttttatgga agtataccat
     7561 gtaccagaaa aatgactaga gaagaaattt gcaatgaata cgaaaaggaa actggaaatg
     7621 taataattga agaatttatg ggaagaaatc caattcattg tccaggaata attgtaaatg
     7681 accacggacc ttttacatgg ggaaaagatg caaatgaggc tgtacacaat gcagtggttt
     7741 tagaagaagt tgcaaagatg gcttattata cagagttaat gagtcctgat aatattatgg
     7801 ataaggttct tatgaataag catttttcaa gaaaacatgg aaaaaatgca tactatggtc
     7861 aaaaatgatt atctggaggt gaattatgct agagtcttta aaaggtaaag ttgtggaaat
     7921 agcaattaaa gcagagcatt atggtttatg taaagaaaag actggtaatt ttagtattag
     7981 agataaggat actggatata tacttataac tccatcaggt ataggaagag aagaattaaa
     8041 agttgaacat atttgtgtat tggatttgaa tggaaatgta atagaagttg agaaaggtat
     8101 taagccaagt agtgaggtat tgatgcatat tgaaatatac aaaacaagag aaaatataaa
     8161 tgctatatca catactcatt ctttatatgc aacagcattt tcagtagcaa agaaaccaat
     8221 tataccaatt gtatatgaat cagttaatta tggcggttat gtagatattg ctccatacga
     8281 aagaccagga actttaaaat tagctaagag tgtttcaaaa ttattgatag atacagatgc
     8341 atgcttatta gaaagacatg ggttgacaac tgtaggtaat gatttaaacg aagcattatt
     8401 aaaatcaaga tatgtggaag aggtagcaga aatatactat aagtctttag tattaaatca
     8461 atttaaagaa cttgaagtag tagatgttga tgagtttaag tcttggaaat acccagaaga
     8521 aataaagttt taatctaaat aattttcatt gaagtggtta attaaaatat atgtattatt
     8581 tatatcaatt actatattgt attatgttaa ttacggtatt ttgtaaatat atttattaca
     8641 ttttttcaaa aaaatataga atccgaaagc catataaaat tgacttggga ttctatactt
     8701 tttatttatg catttttctt tatctgattt tccggagaat ttatatgttc aaaatatttt
     8761 ttagtttcag atactattat aggtgataat gcaagtaaag ctataaggtt aggtattgcc
     8821 ataagtccat ttacaatatc agcaattatc caaacaactt ctaactttaa gaaagcacct
     8881 aataacacca tagccacaaa tacagttcta tacccattca tgccttttac accaaacaga
     8941 aattcaaaac atctttctcc ataataactc catcctaaaa ttgtagtaaa tgcaaatagt
     9001 gttaaactaa ttgtaagtaa tattggtcca aatgtaggaa ttgctccgtt aaaagctgct
     9061 tgtgtcataa cggctccatt taggtctgaa caccatactc ctgaaataac aagagagaat
     9121 ccagtaagag tacagattat aatagtatct ataaaagtac ctgtcataga tataagacct
     9181 tgttccgctg gccattcagt tttagcagca gctgcagcta ttggagcact acctagacca
     9241 gactcatttg aaaatacacc acgagcgata ccatttctaa ttgccagtga catagttgct
     9301 cctaaaaaac caccagtagc agcagtaggt gtaaatgcac ttttaattat taacataaaa
     9361 gtttcaggga ttttattcca aaaagatatt aaaatgattc cacagattac aacatatata
     9421 actgccataa aaggtactat tttagtagct acattagata tacttttaag tcctccaaat
     9481 acaaccaaag ctacaaatac tgctaataca acccctgtaa ttctagggtc tatcccgaaa
     9541 ctattattta ttgcatctgt tattgaattt acttgagtaa aagtaccaat tcctaaaagt
     9601 gcaactagta taccacttat agcgaaaaat attgcaagag gtctccattt ttctcccata
     9661 ccatttacta tgtaatacat tggcccacca gacacttgac catttttatc tttagctcta
     9721 tattttattg caagaacacc ttctgaatac ttagtagcca ttccaaagaa tgctgctatc
     9781 cacatccaaa aaagtgcacc aggtccacca gctttgatag ctgtagcaac accaacaata
     9841 ttaccagtac caactgtagc agctagagca gtacataagg ctgcaaaact tgaaacatca
     9901 ccactacctt tgttttcagc agagaatatt aacttaagag ctcttggcag tttagttatt
     9961 tgaacaactc caagcttaac agtaagtagt ataccagtac caactaatag tactagtagg
    10021 ggtggtcccc aaataaaagc atcaactttg tttaaaatat caattagatt cataaaaaca
    10081 ctccctcatt tttttgcttt acataatcaa tagaattttt aatgaagtaa ttgacaggaa
    10141 ataagtataa tttattaata atttctagtg taataaaaaa agctaaatct aaaaaagatt
    10201 cagctctgga tgtacactaa ttagcaacca aaataaaagt attagtaaat actgtttttg
    10261 gtagataatc cctgtccttt tgcctgagag attgagcata aaagccttgc cccttcggcg
    10321 cccataatgg gtctctccag agttccgtaa attttacagt cctactttta aaagtacctg
    10381 agagtgttac tccttcggtg tgctatatag caactcttct ataaaatctt aaccggatat
    10441 tgatttgtca tagcttattg tattaataat attaatataa gtttttatgt tttgtcaact
    10501 aaaaaattaa aaaacaaaga aaaaatataa aaatgtaagc ctatttacga attataaact
    10561 tagagagatg agtaatatac gtgaaaatga attttgtaat acacatacaa atgaagtgta
    10621 aaataagttt taaataaaaa aataataaaa tcttaaataa ttaattagat atgattaaat
    10681 tatggtaata ttatagttac gatattaaga aaagttctat ttagttagat tttaagcgta
    10741 atatgagatt atgtgtagag aaaggatgta tttttagatg ggaaaaatat tagtgctagc
    10801 agaaaaacct tctgttggaa gagatatagc aaaagtttta gggtcaaaaa atgaaaaaaa
    10861 tggttatata gaggggccaa agtatgtgat aacttgggca ctaggtcatt tggttacact
    10921 tgctgacccc gaaagttatg gagaaagata taaatcatgg agcttagaag acctgccaat
    10981 acttcccaag taccttaaga ctgttgtaat aaaaaagagt ggaaagcagt ttaatacagt
    11041 taaatcacaa atgaatagaa atgatataga tgaaatagtt atagcaacag atgcaggtcg
    11101 tgaaggagaa cttgtagcaa ggtggataat agaaaaatca caggtaaaaa aacctataaa
    11161 acgtctttgg atttcttcaa gtacagacaa agcaataaaa gacggatttg caaaattaag
    11221 aagtggaaaa gaatatgaaa atttgtatta ttcagcaata gcacgagcag aagctgattg
    11281 gattattgga ataaatgcaa ccagagcttt gacaacaaag tacaatgcac agctttcttg
    11341 tggaagagta caaactccta ctcttgcaat gttgcttaaa agagaagaag aaataagaaa
    11401 tttcaaacct aaagagtatt atggattaga attaattgct acaaagggaa attcagatat
    11461 aaaatttatt tggaatgata aaaacaataa ttcttcaact tttagtaaag aaaagataga
    11521 atccacatta aaaaaagtaa aaggtgtaga cataaaaata tctgaagtaa acaagtcata
    11581 taagaaaaaa tattcacctg cactctatga tttaacagaa ttacaaagag atgcaaataa
    11641 aatgtttgga ttttcagcaa aagaaacttt atctataatg caaaaactgt atgagcatca
    11701 taaagtactt acttatccta gaactgattc tagatattta acatcagata ttgttgggac
    11761 attaaaagat agaataaaag ctgtaaatat tagtgattat tcaaggattt gtaataaatt
    11821 attaaaaagc aatatcacag ctaataaatc gtttgttgac aattcaaagg taacagacca
    11881 ccatgcaata ataccaacag aacagaaggt attcatgagt gattttagtg acaaagagcg
    11941 aaaaatatat aatcttgtag taaaaagatt tttagcagta ctatctcctc cttttgaata
    12001 tgagcagaca actataagtg caactgtaga aggagaaata tttaaggcta agggaaacaa
    12061 agtaataaat ttaggttgga aagaaagtta taaagatatt gaagatgaag atgagtatga
    12121 aagtatacca aatgttttag aaaaagatat acttaaggtc aagggattaa agataacttc
    12181 aggaaaaaca aatccaccac catacttaaa tgagggaaca ttattgactg ctatggaaaa
    12241 gaatagtctg gggacagttg ctacaagagc agatataata gaaaaattat tcaattcatt
    12301 tttaatggaa aagaaaaata aagatatata tataacatca aaaggaagac aattattaga
    12361 tttagttcca gtagacttaa agacaccaga acttacagct tcatgggaaa agaaattatt
    12421 ggatatttca aagggaaaac ttagtaaaaa tacattcata aatgacatga aagattattc
    12481 aaaagatatt gtactagaag taaaaaatag tgataataaa tttaaacatg ataacttaac
    12541 taaaaataga tgtccagaat gtggtaaatt tatgttagag gtaaatggta aaaaaggtaa
    12601 gatgttgatt tgtgaagata gagagtgtaa tactagaaaa actatatcac aagtaaccaa
    12661 ttcaagatgt cctaactgtc ataaaaaatt agaacttcgt ggagaaggtg aggggagaat
    12721 cttcacttgt agttgcggat acagagaaaa gttgtcttct ttcaacaaga gaaagcaaga
    12781 agaaaaacag aaggcatcaa aaaaagatgt caatcaatat ttaaaaaatc aaaataaaaa
    12841 tcaagagaca tttaataatc catttgcaga agcattagct aagctaaaaa acaaataatt
    12901 ttaaagttat tattatacac aattataact ttaactgtat attagttata tgtactgata
    12961 taatatagtg tatagtttag gcatgaaagt agggctgtta aatggattta agtggcatat
    13021 ttaaatacta ttgtaaagag tgtgaaaata catggaataa ttcgagtgtt gaattatttg
    13081 agaatataga aacgtatagt aaagattcac aaaaaaagag ggaaaaagaa ttagataaat
    13141 tgatgaatac aatatcagtt catttagaga ggtatccaag tgatgctgta ttgagaaaaa
    13201 tgtgggtaaa aaagggcgag gttttcttac aaaagacatt ggaaaaagaa aatattttta
    13261 agttagaaaa aatggatgta gaggatagaa aaaaattttt agatataaca aaacagttta
    13321 ttagagatgc tagaaaattt gatagtgatt taccgatagg tgatattatg caagctatga
    13381 ggaatgtatg gatttcaaat gcattacaat tattatttgg taaagaagta tattattcaa
    13441 aagctaactt tgcatatagt atgttatatc catatactga taattattta gacaatacaa
    13501 gtatagataa aaatgataag attttattta ataactggtt agaaaaaagg ctcctgggag
    13561 aacacattaa atctaaggat tatcatgaaa gtaaagtatc tcaaatgata gattatattg
    13621 aaagtgtata ccctagagaa aagtttacag aagtctatga atcgttatta ttaatattta
    13681 aaagtcaagt aaatagttta aaacaacatg gtaaggaaaa tcatttgtgt aaagaagatt
    13741 tattatccat ttctattgaa aaaggaggtt catccgtttt agtagatggg tatttaataa
    13801 gtggattgat gacaaaggaa gaaatagagt tttgtatagg atatggattt ttattacaaa
    13861 tatctgatga tttacaagat ataaaagaag atttaaaata caaccataaa actattatta
    13921 cagagatggc aaaagggggt actttagata aagttgtaaa taaactaata aattttacta
    13981 ttgagttaat agatagtttt aaaattaata ataaaaataa atctgtaata actatgataa
    14041 agaatgattg cttaatgtta attttatttt ctgtagttta taatgctgaa tttttttctg
    14101 taggatatat aaaagaagta gagaaattta ttccatatac aatagattat tcattggaga
    14161 ttgaagaaaa aataaaagaa aaatttaaaa atatagatgt tttaaataat gaaaatgaat
    14221 ataaagaaat gattgatatt atttgttcag agtagaaaaa aagggagtgt cttagaatag
    14281 actttttagt ttatgaggca ctctttttgt ataaaatcaa atgtattttc atcattctaa
    14341 caattaaaaa gaggcttatc caatttgaga cagccctttt taatttaaat aaaaatgtca
    14401 aggttaaact atttaaatgc agtaataatc ccattatcat gtaagttttt tatgattgta
    14461 gctgcgatag ggaatccaaa taatccagct attccaaata gttgagctcc aacaaacata
    14521 cacattaatg ttattaaagg atataggcca atttggtgtc ctactacctt tggttctaat
    14581 atttgtctaa caacagttat aattatgtat agtattaaaa gtcctattgc taaattaata
    14641 tttccattaa tgagtgagat aatcatccaa ggtgtcaaaa tacttcctgt acccaagact
    14701 ggcaaaatat caacaagtgc tatgataata gatatagtaa atgcattttc aacctttagg
    14761 atagtaagtc caataagtag ttctatgaat gtcactgata gtagtattgc ataggctttt
    14821 agtagtttta aaagagcatt aatactgcta ttttttatat ttattatcat aaattgactt
    14881 ttttctggaa attgttttaa gataaagttt actatttttt gataatcaaa agtaaagaaa
    14941 aaagaagcaa ttaaagtaat aataagtttt aacataaatg caggtgtttt acttgcaata
    15001 ctagcaatag cattgagtgc atttgttgaa atagaagcta ctaaagacat catagattga
    15061 cttatattat cccaaccaag tattaatgct aggttaacat taggtgtact agcatctatc
    15121 tttgaaaata aagtatttag cgttggttgt atacttgatt tatatatttc aggaagtcta
    15181 aaaaatacat cacttacact tgcaaatatc ttcacaccaa agctaactaa taaaaatata
    15241 cacaacccat aaaaaactaa aagtattact atagaaataa gtgtacgatt taaatttgta
    15301 ttatttgtaa taagtcttat aataggtctt aagatggatg caatgataaa actgattaca
    15361 aaaggcatta ataatggcaa taagaatttt aatacaatat atacaatact tgttattaaa
    15421 actacatatg taaatttaat tataaaattc ttttgctttt gaatgtccat aatataaaac
    15481 ctccaatgta tttgatatta ttctaaatat ggtctttttt acagaaaata tactactgta
    15541 tattgattat aaactataaa gcctatgtaa agtaaataaa ttatgtaaaa tttaggttaa
    15601 ttttgtataa agagatactt ttatatttta caattaataa ccaaatacta aaaaatattt
    15661 taaataaaga acttaaaatt gtgataaaat taatttatag atattaaaaa aagtataata
    15721 tatggttttt aagaggtgct aagatgaaaa attttagaga tttaggtggg aacaaaactg
    15781 aggatggcag aactgtaaag aagggattat tttataggtc tgcaaaatta tcaaatctaa
    15841 gtgaaaatga tataaagatt ttaaaagagt taaatattaa atatatattt gattatagaa
    15901 gtgatgaaga agctcgtaaa catccatcaa caataatatc aaatataaaa aatataagaa
    15961 taccagcaat gagagagtta gaagaatcag gaggaagttt tggttctatt gaagatatga
    16021 ttgatggatt atttgaaaaa gatggtgcat ttaatatgtt aaataatagt tactataatt
    16081 tgcctataaa taatccgtca tataaaaaac tagtagaact tataagagat tattctaatt
    16141 taccaatatt aaatcattgt acagctggta aggatagaac tggtgtgggg agtgctataa
    16201 ttcttatgat tttaggggtg tcgagagaga atataatgaa agattatcta aaaagtaatg
    16261 attttgcaga taaagagata gaaagattta tagaatataa acctaaattt aaggatattc
    16321 caaaagaaaa tttaaaatat atatttggtg taaatgaaga gtatatgaaa actgcattta
    16381 gaagaattga tgaagaatac actagtgtag aagcatattt atatggagaa tttaatttaa
    16441 ataaagaaga aataagaaaa ttaagaaatc aatatctaga gtaaaaggtg agtttatgag
    16501 tttaaatata acagcaagac agataaatat aataaaaata ttgttaaatt caaaagatac
    16561 aattagtggt cttgccttaa gtcaagagat aggatgttct tcaaaaacta tacaaaatga
    16621 aataaaggat attaataaac aacttagaaa tggtaaaatt ttgtcaataa gaggagttgg
    16681 ttataaacta gaaggaagtt ttgatgaggt gaatctaaat agcaatctct atgatgatgt
    16741 agatagagta gaatatataa tcagaaaaat attaacctta agtaatgaag aaaaaaatac
    16801 tataaaactt gaagatttag ccgattctat gtatgtaagt gtttcgacgg taaaaaatga
    16861 tttaaaagaa gtaaaaagaa tgttggaaaa gtattcaata tcagttaaat ctaagcataa
    16921 acaaggtata tgtgttttgg aaagtgaaga taagatatta aattgtatag ttgacttaag
    16981 taataagaga gataatcaat tgagtttaaa tgatttttta aatgatgatg taaaggaaaa
    17041 aaaactacta ataaaaaaat tattattaga catattaaat gaaaatagat tggtattaac
    17101 tgacatagaa tttaagagtg tgttaaataa tatattagtt atgttaagca gacataagta
    17161 taatgaaaaa gaatttatag agaattatat agaaaattat aggagcaaga gaagctcaat
    17221 tttagaaaat gaacaaaaca aagaagttat aataaattca ataaaaacat tttgtaaaaa
    17281 tttaaaatta gctacatcaa tagatataag taatgataag gtatttgaag agtttttata
    17341 taagcatatt agtagtttat gtaaaaaaat aaagttaggt ataaatcaat cacctataat
    17401 ggctcaagat ataaaaataa aatatccatt tgcttttgaa ctagctaata tagctaaaaa
    17461 gacaatagaa aaagatttga agatagaaat taatgaagat gaagttgcaa acatagctct
    17521 acatatagga ggagcagttg aaagagcatc ttataataat aaagataagg ttttcaaaac
    17581 tataatagtc tgtacttcag gcataggcac atcaatgtta ataaaagcta aattagagaa
    17641 tatatttaag gaaaaattag aaatactaaa gattattcct tcatacttag ttgaatatat
    17701 aaaagttata gatgttgatt ttgtaatatc tactgttcct attgaagtag gagatgtgcc
    17761 agtaataaat atatctccaa ttttaagtga gaaagaaatt aaattaattg aaaaatatat
    17821 agaaacagga aatatatacc tagatttaga tataaaaagc ctgtttgata gtgaattatt
    17881 ttttactgat ttggtgttta aaaggaaaga agaagttata gattttatgg caagtaaact
    17941 tgtacaaaag ggctatatag atgaggatat gaaaaaatct tactttgaga gagaaaaaat
    18001 agcaacaaca gaaataggga atatggtaac tataccacat ggagctattg gaaaaatttt
    18061 tgataataaa atagcaattg gtatattaaa gaagcctatt tcttgggaaa tatcagatgt
    18121 tcgtttaata ataatgttag ctcttgataa agataaaata ttagattatg aagtaatatt
    18181 ttcaaaaata tataaaagag tagattctat agcaaaagtt ataagtatat gtgaaaatag
    18241 tagttttgaa aaatttgtaa atttatttaa ttagatgggt agtattgtat atacacctgt
    18301 aaatttggat aatataaaac taaataaggt aaaaacgata aatttctctt ttaattaaga
    18361 gaaatttttt gactttcaag gatattaata taaagttaag atataaacat aaagcaaata
    18421 aataaaaaag aggtggcaat atgatttata cagtgacatt aaacccatca atagattata
    18481 tagttaagct agatgaacta aagacaggaa gtacaaatcg tgtaaatgaa gaatacgttt
    18541 atccaggagg aaaaggtata aatgtatctc gcatacttaa ggaacttgga aatgataata
    18601 catctttagg ttttataagt ggctttactg gagaatatat aataagaaca ttggaagaaa
    18661 aaaaattgaa gacagatttt ataaagataa aaagtggttt ttcaagaata aatgtaaaaa
    18721 taaaggcatt aaaggaaaca gagattaatg gacaaggtcc aaatatagat gatgaagaca
    18781 ttgatatttt atataaaaaa cttgataagc taaatcaaga tgatatttta atattggcag
    18841 gaagtatacc atctacttta gatgaaaaac tatacgaaaa tataatggct agattagaaa
    18901 aaaagaatat aaaagttgta gtagatgcaa ctaaaaatct tcttcttaat gttttaaaat
    18961 ataaaccttt tctaattaag cctaataaca acgaattaga agaactattt ggtgttaaat
    19021 taaattcaat tgaagacatg gtaaaatatg ctaaaaggtt aaaggaaatg ggagccataa
    19081 atgtcttagt atcaatgggc aaagacggag cattgttgat tacagaggaa gatgaagtgc
    19141 ttataagtga tgttcctaaa ggaaaagtaa aaaattctgt aggagcaggt gattcaatgg
    19201 tagcaggatt tatatctggg tatttgaata ctgggaaata tgattatgct cttaaattgg
    19261 gagcagcttc aggtagtgca actgcttttt catatgactt agccaaaaga gaatacattg
    19321 ataaattggt aaatgaaata tctgtaaagc agttttaaaa tattaaaaaa tatatagaaa
    19381 ggtaattaat atttatgaaa attatagatt tattagatga aaagtcaatt aagttaaatt
    19441 taaattcaaa aaccaaacaa gaagcaatag aagaattagt agatttggtt gcaaacagtg
    19501 ggaatttaaa tgataaagaa aactacaaaa aagcaatttt ggctcgtgaa gaaatgagta
    19561 caacaggaat tggagaaggt gtagctatac ctcatgctaa aaattcttct gttactaaag
    19621 cttgtatagc agcagcagtg tcaaaagaag gtatagatta tgaatcgttt gacggaagtt
    19681 tatcaaattt attttttatg atagctgcac cagacggagc taacaatact catcttgaag
    19741 tattatcaag actatctaca atacttatgg atgaagactt tagaaataaa cttataaatt
    19801 caagttctga aaaagaattt aaggaaataa tagataaaaa ggaaagagaa aaattcagtg
    19861 aagaatatca agatgagaaa gttgctgaaa aaaatataat agatactaaa gagaatgatg
    19921 ctaataagta tcctaaggtt ttagcagtaa cagcatgtcc aactggaata gcacatacat
    19981 ttatggcagc tgaaagttta aataaaatgg ccgaaaataa aggtgtatca ataaaagtag
    20041 aaactaatgg ttcagcaggt gttaaaaata agcttacaaa agatgaaata gaaaatgcaa
    20101 catgtataat agtggcagca gataaaaatg ttgatatggc tagatttaat ggcaaaaaag
    20161 tcataaaaac taaggtggct gatggcatac acaaagcgga agaacttata gacaaggctg
    20221 taaatggaga tgctccaatt taccatggag gagatggaag tcacaacgaa tcaaatgaag
    20281 aaagtgaaag tggatttaga aaagtataca aacacttaat gaatggtgta tctaatatgt
    20341 taccatttgt tattggtgga gggatactta tagcgatagc ctttttactt gatgattatg
    20401 ctattaaccc ttcaaacttt ggttcaaata caccgatagc agcatttttt aaaggaatag
    20461 gagataaagc ttttggattt atgttacccg tacttgctgg atatatagca tactctatat
    20521 ctgatagacc agcatttgtt gtaggatttg taggaggtgc tctagcaggt gatggtggtt
    20581 caggcttttt aggtgcacta cttgcaggtt ttatagcagg ttatttagta gaagggctta
    20641 agaaaatatt tagtgtacta ccagcttcat tggaaggaat aaaaccagta ttgttatacc
    20701 ctttacttgg aacacttttg atgggaatta taatgacatt tttagtaata cctccagtta
    20761 cagcaattaa taatgcaatg attggatttt taaatggttt aggtggaact tcaaaaatag
    20821 ttttaggatt agtattaggt ggaatgatgg ctgtggatat gggtggacct gtaaataaag
    20881 cagcatatgt atttggagtt gcatcacttg aatcagggca gtttgaaata atggcagcag
    20941 taatggctgg tggtatggtt ccgccacttg ctatagctct tgcaactaca ttttttaaga
    21001 atagatttac aaaagaagaa agagattctg gtaaagttaa ttatgtaatg ggattatcat
    21061 ttgttactga aggagctata ccttttgcag ctggagaccc acttcatgtt ataccagctt
    21121 gtgttggagg ttcagcagtg gcaggagcac ttagtatgtt gttcaatgca gctcttcgtg
    21181 cacctcatgg aggagtattt gttataccag tggttaccca tccatttgca tatatattag
    21241 ctatagctgt aggtgctttg gttggaatga tgcttttagc attacttaaa aaaccattaa
    21301 atcaagaagt atagtaatta aaggatattc aagaacttaa aatatgatgt tttataaaaa
    21361 ttaatatatt gctgaattat tttaaaataa gaattttatg aggctgttat gtaacgattt
    21421 gtaattgtta tcttgacagt cttttatttt tatataagta aaataagtgt gtcttgcttg
    21481 atttatattt aaattgtttt taagttag
//
LOCUS       DAEPXF010000059        21360 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain HB27
            SAMN13376389-rid12479423.denovo.063, whole genome shotgun sequence.
ACCESSION   DAEPXF010000059 DAEPXF010000000
VERSION     DAEPXF010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13376389
            Sequence Read Archive: SRR10518432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 21360)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21360)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001069624.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001069624.1
            Genome Coverage       :: 315x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/15/2021 01:54:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,165
            CDSs (total)                      :: 4,076
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 89
            rRNAs                             :: 10, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 10 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 19 of 69
            Pseudo Genes (incomplete)         :: 44 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 10 of 69
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21360
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13376389-rid12479423.denovo.063"
                     /strain="HB27"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="China: Hebei"
                     /collection_date="2017"
     gene            complement(148..828)
                     /locus_tag="KRL67_001846"
     CDS             complement(148..828)
                     /locus_tag="KRL67_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBH1522726.1"
                     /translation="MYRILLVEDDIDLSKEIALALEKWGFKVELIDDFEIVLDEFMDK
                     KPDVVLLDVNLPLYNGFYWCEKIRAISNVPLIFLSSRDSDMDLIMGINNGADDYITKP
                     FSIDILVTKINGIIRRVYNYSDSNSILYCEDLMFDVGKGIIKHKYKDKSIELTKNEIK
                     ILTLLLKNKNRVVSRESLMMTLWDNDEFVTDNALTVNMNRLRSKIKELGFDDFIKTKK
                     GIGYIIQC"
     gene            complement(980..2887)
                     /locus_tag="KRL67_001847"
     CDS             complement(980..2887)
                     /locus_tag="KRL67_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HBH1522727.1"
                     /translation="MSILGIAYNNFKSNIRTYLAFFISMVFSVVVLTNFELLRYGDAI
                     NVLQEENKKFTLSVLVAVIMILSVFLFFFIWYATNIFFKNRSKEIGILSFMGLDLYTI
                     GKIYFVENMLIGISSCITGIIIGIITSRFFQVVIIKLSGFNIKVSNGLSIEAILYSTL
                     IFISIFIIMAVKGFITICRSSVINLINISKKQEKIPKVGIGIYILAIISIIVIGYGYY
                     LSTNIRTGDITSVLPIIGIIIVGTYGLFKSVMPVIFDIIIKNKKILFNGNNIIAINNI
                     NYRLNKNYKTYAIIAIIITTTISTLGASVAMKRIHKNAQEQRNVYTISIVSMDKDEVN
                     KKQIENIIKDTNKIKYSVNPELTILKKTDIKEAYDTTDTIMKYSDFCNTLKVNGNDKE
                     LKEYGERLVSGNNVIELKSPQTIASIATKEAKIKLDGQLYNILRGEVKVPVLGTGLNQ
                     SIIVVNDDTYFKLRDKGQQVYFYGAKVFQEENSQVMFNNIEKNLSLKNGYIQNGYKAQ
                     GESQWMKFAYVVLVFLFLVFAIVAGSIIYMKIYSDAYEDKEKYKILLKVGTTEKDINR
                     AILKEVAIFYTLPMLSATISSYFALRLAGDLLMTDLFGIYILSLVICLIIFIIYGAVS
                     VNKFKSVVYKN"
     gene            complement(2888..3640)
                     /locus_tag="KRL67_001848"
     CDS             complement(2888..3640)
                     /locus_tag="KRL67_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBH1522728.1"
                     /translation="MKEILKIKNISKDYGIKGFKTNVLKNISLTVNEGDFIAIMGPSG
                     AGKTTLLNLMSTLDKQTSGEIILDGINISKVKNNELSKLRREKIGFIFQDYNLLDNMK
                     LMDNIALPLALGKKKSKEIEAKVFSIAKKFGLENHLDKYPYQLSGGQKQRGAAARSLI
                     TNPAVIFADEPTGALDSKSAYELLESLEKINRENNATIIMITHDPLTASYSNEVYMLN
                     DGNIKCKLNKGNSRKEFYGKIMDMLASMGGEM"
     gene            complement(3958..5112)
                     /locus_tag="KRL67_001849"
     CDS             complement(3958..5112)
                     /locus_tag="KRL67_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBH1522729.1"
                     /translation="MINKAIKFKFIIFYFFSYIIFAFASTKFTPFLSKLGYSAFERGI
                     ILSSYAITNILFQLLFGVLADKYQTMKKIVLISLSVYGIASVIMFSTESATFIIYLLL
                     VALSGGLLNTCCSLYDTWIIGCGDKINNYLSFIKTFGSIGWAIGSMVASYLILRFSYS
                     GLSVAIGLIVVLLIINILLLPDIEKINKKINVTSKDIIELLKDKKYVLLVCILFLLYS
                     MVVANNCTVIDKMIALNATNSQISLKWSLQSLLEIPTYLAGSYLLKKYSSLNLLKLSA
                     IMITIQFLLFALTNDSNIIIILCVFQVFSTPLILITSKRLIFEVSPKKLRSSSQLIAL
                     SIFMGGSSLIIPTVAGFLSVNIGYNYTLMVLSLFGCLAYGLIVSLNYLQE"
     gene            complement(5115..5582)
                     /locus_tag="KRL67_001850"
     CDS             complement(5115..5582)
                     /locus_tag="KRL67_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HBH1522730.1"
                     /translation="MFFKRKKEVMTINSYIDGKIINLEEIPDKVFSTKVMGDGFAMIA
                     KDNILKSPTKGVISFIAQTNHAIGIKLQNDVQILIHIGLDSATTKKENFKLEVKQGQE
                     VNMGEKLVEIDNKLLENTLYIPLVIVENPNTVKYHLSNLNQEGNSGIKVINFD"
     gene            complement(5596..6084)
                     /locus_tag="KRL67_001851"
     CDS             complement(5596..6084)
                     /locus_tag="KRL67_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH1522731.1"
                     /translation="MITNKIKNITAVLKDENIFLSVVMICFLILPVLYIVYLELTSLF
                     TNISFSQLLNNDVNASLNLISNFSAVYSAYVIYNIKKNKEIKENMIAFYLVLLGQIFQ
                     IYTITIVIMFTYIYKFVGIKQIISSIKELQWGKSYKALFSSIAVCLISLFVMIIRIKL
                     IM"
     gene            complement(6098..7429)
                     /locus_tag="KRL67_001852"
     CDS             complement(6098..7429)
                     /locus_tag="KRL67_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-alpha-glucosidase"
                     /protein_id="HBH1522732.1"
                     /translation="MERKRQRILIAGGGSTYTAGIVTMLIESVDKFPIESIKLYDNND
                     ERQRKVAEACAIIVREKNPEIKFSYTTNPEEAFTDIDFVMAQIRVGLYALREQDEKIP
                     LKYGVVGQETCGAGGIAYGLRTIGPIIEMIDYMEKYSPNAWMLNYSNPAAIVAEACRV
                     LRPNSRIINICDMPVCLEEIFARVLGLNSRKDFDVRYYGLNHFGWWTSIKDKEGNDLM
                     PKLQEYCAKKGYEEFTPQGQHKESSWLETMRAAKDLLEIEPATLPNTYLKYYLMADET
                     VEHANPNYTRANEIMDRREKDTFEECERIIKKGTARDTWFDASEHSQFIVELACALAF
                     NTQERFLLIVPNNGAIENFADDAMVEIPCLVGKDIVEPMSIGKIPTFQKGLMEQQVAS
                     EKLAVEAWIEKSYQKLWQSFTMSKTVPSAKVAKEILDEMIVANKDFWPTFK"
     gene            complement(7443..9008)
                     /locus_tag="KRL67_001853"
     CDS             complement(7443..9008)
                     /locus_tag="KRL67_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003233606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HBH1522733.1"
                     /translation="MMKKIQRFGGAMFTPTLLFAFAGIMVGFSIVFQNQSIMGSLATP
                     ENIWYQFWGVISSGAWMVFNQLPLLFAISLPIALAKKQQARACMEALATYLTFNYFVG
                     SMLSFWGKSFGVDFAAEISAGSGLVSIAGIKTLDTGMVGALLISGIVIYVHNRFYDKE
                     LPDFIGLFRGSSLVVAICFFVMIPVALLTCFIWPHIQNVIRHLQTFFINSGNIGVWCY
                     AFLQKILIPTGLHHFVYAPICYDSVVVPGGTSVYWATHIQDFQTSAKTLKEMYPIGFS
                     LSGLSKVFGSLGVFGAFYVTAKPEKKKKVLGLMIPATLTAVLTGITEPLEFTFLFVAP
                     LLFLVHAFLDACLQTISFALGVVGDFGGGIINWVVLNWLPLGMYHWKVYVVQVVVGII
                     FSFIWFFVFAFLIKKFDMKTPGREEDSEETKLYTKNEYLETKDEKGNKLSKASQQASE
                     YIKLVGGAENVVDVTNCATRLRLTLKDDSIISKEEDFKAVGAHGLVHNGKAIQIIIGL
                     SVPSVREEFENLL"
     gene            complement(9177..9962)
                     /locus_tag="KRL67_001854"
     CDS             complement(9177..9962)
                     /locus_tag="KRL67_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HBH1522734.1"
                     /translation="MRLEEMIFFNYNKLNDTDLLIWKYITANKKHCSKMSINELAMKC
                     NVSRATITRFVKKLDLKGFSEFKVLLSWESEKVHVIENQSFDIACNSIIKYVEDQKNK
                     DYDELCKLLYESRTIYAYGSGDIQNVVAKQIKRMFLSCQEIIYDFEGRTFDDSFYNIV
                     SKNDVMIIISLSGENENTLNIARRLKLIGTKIISITEFKNNTLTDLSDESIYISATNI
                     SFLQMHPSYKLTMLYFILIELLFIKYSIYKRHRMITEGIDCKL"
     gene            complement(10128..11327)
                     /locus_tag="KRL67_001855"
     CDS             complement(10128..11327)
                     /locus_tag="KRL67_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase S41"
                     /protein_id="HBH1522735.1"
                     /translation="MKKKVLFLVIILAFIILLGNSTYSQKNQLSEEEKVDDFNYVYNV
                     VKTGYPYLGVNKRLNNIDWLANKDEYTKRIKNTENDEEFIEELSSILSDLNNKHTEVI
                     DNKKRYELFKKSYSNNDWYDFFNDKKVVDRYDSMNPKIKMPDDIFVKKELILKDVIRG
                     QVGYMYLPSMSSKNGSINNDLKIIGDYINTLEKHKALIIDIRGNLGGSDRYWKGIVSK
                     LIKNDVKINGYRIYRNNNEIVKKYTNARNIKLNPVESLPANVKENAPKEIIKDFNGFE
                     DTSYTIKSTDDLKFRGNIYLLVDRKIYSSSESFAMFCKETKFATLVGETTGGDGGCID
                     PVLFNLKNSGLIVRMASCMYLNKSGVCDEEFKTTPEFKVKDCKRTADFKEDNCIRKAL
                     ELENINY"
     gene            complement(11850..13187)
                     /gene="xylA"
                     /locus_tag="KRL67_001856"
     CDS             complement(11850..13187)
                     /gene="xylA"
                     /locus_tag="KRL67_001856"
                     /EC_number="5.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylose isomerase"
                     /protein_id="HBH1522736.1"
                     /translation="MSEIFKGIGQIKFEGVKSDNELAFRYYNPEQVVGNKTMKEHLRF
                     AMSYWHTLCGEGNDPFGVGTVERPWNNITDPIEIAKIKVDAGFEFMSKMGIEYFCFHD
                     RDIAPEGRDLEETNKILDEIVEYIKANMEKTGIKLLWGTANMFGNPRFVHGASTTCNA
                     DVYAYAAAQVKKAMEITKYLGGENFVFWGGREGYETLLNTNTELEMDNFARFLQMAVD
                     YAKEIGFTGQFLIEPKPKEPTKHQYDFDTATVLGFLRKYNLDKYFKVNIEANHATLAG
                     HTFQHELNIARINNVLGSIDANQGDLLLGWDTDQFPTNIYDATLAMYEVLKQGGIAPG
                     GFNFDSKVRRASFEVEDLFLAYIAGMDTFAKGLLIAHKLLEDEVFENFTKERYASFSE
                     GIGKDIVEGKVGFKELESYALQMPVIKNKSGRQEMLEAILNRYIYEVDTISNK"
     gene            complement(13287..14834)
                     /gene="xylB"
                     /locus_tag="KRL67_001857"
     CDS             complement(13287..14834)
                     /gene="xylB"
                     /locus_tag="KRL67_001857"
                     /EC_number="2.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylulokinase"
                     /protein_id="HBH1522737.1"
                     /translation="MKLEYVLGVDIGTSGTKTVLFDKLGNTIKSCTYEYPLIQEKSGW
                     AEQDANDWWKAVVESIREVVQSSNVSSECIKGIGLSGQMHGLVMLDNEGKTLRNSIIW
                     CDQRTVKECEEITDLVGEERLIEITANPALTGFTASKILWVRNNEPDIYKNTNKILLP
                     KDYIRYKLTGEYATEVSDASGMQLLDIRKRDWSDEVLEKLNIDKNLLGKVYESQEITG
                     YVTRDVASLTGLKEGTIVVGGAGDQAAGAIGNGIVKDGVVSSTIGTSGVVFAYTKEPK
                     IDKEGRVHTFCHAIPNTWHVMGVTQGAGLSLKWFKDNFCQSEVEVSNSLGEDVYEIIN
                     NQVSQVPTGCNGLIYLPYMMGERTPHLDPYARGVFFGLSPIHSKKEIARSIMEGVSYS
                     LKDCMDIIENLNIDVNEVRASGGGGKSKVWRQMQADMFNQDVYTINSSEGPALGVAIL
                     ALVGAGIYENIQKACDAIIKTSTKLEPVSENVDTYKIYHRLYKRIYKSLKDDFRLLDE
                     VVNCKNK"
     gene            15042..16202
                     /locus_tag="KRL67_001858"
     CDS             15042..16202
                     /locus_tag="KRL67_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family transcriptional regulator"
                     /protein_id="HBH1522738.1"
                     /translation="MVTDKYTIREMNERLVLEQIIKNGPISRASIASTIGLNKATISA
                     ITKKLIDESLVHEIGIGNSTHSGGRKPILLVFNKCAGISLSMDIGYDYIFSSLSYLDG
                     TIINSKKLTDIQVSKDNVIQLIDEIINSYNISKIDTPYKVIGLTLAIHGITCENKVLF
                     TPYYNLNEIDLYSILSKKYDFPIHIENEANLTALAENTFSTVHNSLLSLSIHSGFGSG
                     IIINNKLYSGRNGMSGEIGHTIIMPNGKLCPCGNRGCLEQYCSEKKVFEQLSSLENIP
                     KIDSDIVKQLYYEDNQNAKKVIHEFCSYLTIAINNAITTYAPEIIYLNSQIISDIPEI
                     LQITKDMLVSSFNKGINIEISSLGSEASLYGGSAVNIKSFLNIQNLTLINEI"
     gene            complement(16358..16762)
                     /locus_tag="KRL67_001859"
     CDS             complement(16358..16762)
                     /locus_tag="KRL67_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBH1522739.1"
                     /translation="MNSIILMSHGNMAEEVLNSAKMIIGDSIDYPTVNMKQDDGIEGT
                     IEKLKKALEVYKESSEIIIMADLLGGTPCNAALLVANNDSRIKIISGLNLGMVIESAF
                     LDTKELAAKLEKIGKDNISMVEQNASLLSDDE"
     gene            complement(16783..17265)
                     /locus_tag="KRL67_001860"
     CDS             complement(16783..17265)
                     /locus_tag="KRL67_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBH1522740.1"
                     /translation="MEMKGIKNIRIDDRLIHGQVATMWSNKLGVTRLMVVNDAVANNS
                     VQKQVLRMATPAGIASSIITEETAIKNITAGKYEGQNVLLIVKSPVDLIPFVDAGLKI
                     HSINVGNMSSRKDTTVLKPNISVTEEEKEAFKRLLDEGIEITTIMTPDDKKTYLVDIL
                     "
     gene            complement(17285..18094)
                     /locus_tag="KRL67_001861"
     CDS             complement(17285..18094)
                     /locus_tag="KRL67_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HBH1522741.1"
                     /translation="MSDKVSLNKSNEKVKKSVLWRWFFTSSVSSNYEKMQALAYCYAV
                     LPFLKVVYKDNPEKLQSAVLNHLQFFNTNPWVAPYILGINVAMEELSSEETEEAVASI
                     KTGLMGPVAGLGDSLFVVIPWTIFGAIAANMAIDGSPFGILLWIVVSVALKLLSIPLF
                     NAGYTSGTKLISSIEKSLKILTESTSILGLMVVGALIPSVVKANVALNFQQGDFTMKG
                     QEILDQIMPGLVPAILVVIVYWALKKNIKPIYLILGVMVVSIILYALGILK"
     gene            complement(18087..18866)
                     /locus_tag="KRL67_001862"
     CDS             complement(18087..18866)
                     /locus_tag="KRL67_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBH1522742.1"
                     /translation="MEPQLWQLLLIVLYGFFINYEKNSTMFGTYQPVTAGFITGLILG
                     DINTGLYIGGTLQLLSLGISNFGGASIPDYQTASIVATFITITTKQEASVGISIGIPV
                     ALLMVQLDVLRNTIGIWLVHKAEDGAKKGNYKNITYMQMLGVLLTAATTGIPVALSII
                     FGPSLINTILKYTPEWLTGGLTVAGGLLPAVGIGLLLRYLPAKEYFSYLVIGFVLAVY
                     MKVPLLGVALIGGAIALIIYKKNLENQEQQYTVVGGMDEDE"
     gene            complement(18866..20944)
                     /locus_tag="KRL67_001863"
     CDS             complement(18866..20944)
                     /locus_tag="KRL67_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 31 protein"
                     /protein_id="HBH1522743.1"
                     /translation="MIKISEDYIEKRFDNELLRIEAWGKNSLRIRSFVDQNFVDENYA
                     LNEKPKLNKDDITINKNEDGSAIIKNGKIKAVLDHRDRITFYNDKNEILLKEYIRLRA
                     VKHDDGGEDVGTIEITKDFNSTLKLKSREYRPSYSGEFEVTTRFESEPSEKIFGMGQY
                     QHKFLDLKNTVLELAQRNSQISVPFYISSLGYGLLWNNPGIGKVSFAKNMTEWKMFST
                     NFIDYWITCGESPKELNKNYSQVTGTVPMMPENLLGLWQSKLRYRTSEEVLDVVKEYS
                     KRGIKLSSIAIDYFHWPKQGEYKFDLDYWKNPKELVKKLKEEYSVEPIVSVWPTVQSD
                     AENYNDYLENGYLVNVNRGVRMTMQIQGNTVFVDMTNENAREYVWDRIDKNYKQLGID
                     YYWLDVAEPGYSVYDFDNYRYKKGNVLSCGNIYPIDYLKMIYDGLHNDIESVVTLVRG
                     AWAGAQKYGALVWSGDIDSSFEAFNNQVNTGLNMGLAGIPWWTTDIGGFHGGNPKDPE
                     FRELMVRWFQYATFSPILRMHGDRLPHSKPLSNKGGGSMVTGAPNEIWSYGEEVEVIL
                     TKFIKIRESLKTYLKKLMKEAHEDGTPVMRTLFYEFPEDDKTWEVDNTYMLGDEILVA
                     PIMNYKDRSRKVYLPKGHIWENIFSGVSYEGGKTYEVECPLEEIPIFLKQDSSYNFKE
                     LKNILGEVKS"
BASE COUNT         7112 a         3789 c         2274 g         8185 t
ORIGIN      
        1 ttattataaa cagcactaaa aaatctaagt atattaactc tgaatatcta tttttaaaaa
       61 tatctatagc aaataaatag atatttatta tactaactac aactaaaggt agagcaatat
      121 acttagacct accttttaaa tattttacta gcattgtatt atatatccta tccccttctt
      181 agtttttata aaatcatcaa aacctagttc tttaatttta cttctcaatc tattcatatt
      241 tacagtaagt gcattgtctg tcacaaactc atcattatcc catagagtca tcataagact
      301 ttcccttgat acaaccctat tcttattttt taataataat gttaaaattt ttatttcgtt
      361 tttagtcaac tctatacttt tatccttata cttatgtttt ataatccctt tccccacatc
      421 aaacatcaaa tcttcacaat acaaaatact attagaatca ctataattat agactcttct
      481 tattatacca tttattttgg taaccaatat gtctatagaa aatggtttgg taatataatc
      541 atctgctcca ttatttatac ccattattaa atccatatca gaatcccttg atgataaaaa
      601 tattaatggt acatttgaaa tagctcttat tttttcgcac cagtaaaatc cattatataa
      661 tggtaaattt acatctaaaa gtactacatc tggtttttta tccataaatt catctaatac
      721 tatctcaaaa tcatcaatta attctacttt aaatccccat ttctcaagtg ctaacgcaat
      781 ctcttttgat aaatcaatat cgtcctctac taataaaatt ctatacattt gtatattctc
      841 cattatccta atatttcact atgccacgaa ttattaatat cataacatat tttatatgaa
      901 tttattaccc aaattattta gttaatcaac aaggagactc ttattacaag aatctccttt
      961 ctctttatta atatatcaac taatttttat acactacact cttaaactta ttaacagata
     1021 ctgctccata tattatgaat attatcaaac atatcactaa acttagtata tatataccaa
     1081 ataagtcggt catcaataaa tctcctgcta atctcaatgc aaaataacta gatatagttg
     1141 cacttaacat tggcaaagta taaaatatag ctacttcttt aagtatagct ctatttatat
     1201 ctttttctgt agtacctact tttaataaaa tcttgtattt ttctttatct tcatatgcat
     1261 cactatatat tttcatatat attatacttc cagctacaat tgcaaagact aagaataaaa
     1321 acacaagcac tacatatgca aacttcatcc attgactctc accttgagct ttatatccat
     1381 tctgaatata tccatttttt aaacttaagt tcttttcaat attgttgaac attacttggc
     1441 tattttcttc ttgaaatacc tttgcaccat aaaaatatac ttgttgaccc ttatctctta
     1501 atttgaaata tgtatcgtca tttactacta tgatactttg attaagtcct gtccctagta
     1561 ctggaacttt tacttctccc cttaaaatat tatataactg accatcaagt tttatcttgg
     1621 cttcttttgt agctattgat gctatcgttt gagggctttt taactctatt acattatttc
     1681 cactaactaa tctttcacca tactctttta attctttatc attgccattc actttaagag
     1741 tattacaaaa atcactatat ttcattatag tatctgttgt atcataagct tcttttatat
     1801 ctgttttctt taaaattgtt agttctggat ttactgagta ctttatctta ttagtatctt
     1861 ttattatatt ctctatttgt tttttattca cttcatcttt atccattgaa actatgctaa
     1921 tagtgtacac atttctttgt tcttgggcat tcttatgtat acgcttcatc gcaactgaag
     1981 caccaagagt tgatattgtt gtagttataa ttatagctat tatagcataa gttttatagt
     2041 ttttatttaa tcggtagttt atattgttta ttgcaattat attatttcca ttaaataata
     2101 tttttttatt tttgattata atatcaaata taacaggcat aactgatttg aataagccat
     2161 aagtacctac tattattata cctataatag gtagtacact agttatatct ccagttctta
     2221 tattagtaga aagatagtag ccatatccta ttactataat tgatattata gctaatatat
     2281 atatacctat tcctaccttc ggtatttttt cttgtttttt agatatattg attagattta
     2341 ttacactact tctacatatt gttataaaac cttttacagc cattattata aatatagaaa
     2401 taaatattaa agtagaatat agtatagctt ctatgctaag tccattagat acctttatat
     2461 taaatccaga taactttatt atcactactt gaaaaaatct agatgttatt atccctataa
     2521 ttattcctgt tatacaagaa cttattccta taagcatatt ttctacaaaa tatatttttc
     2581 ctatagtata taaatctaat cccataaagg acaatattcc aatttcttta gacctattct
     2641 taaaaaatat atttgttgca taccatataa agaaaaataa aaatactgat agtatcatta
     2701 ttacagctac taacactgat agtgtaaatt ttttattttc ctcttgtaac acatttatag
     2761 catcaccata tcttaataat tcaaaattag ttagcacaac tactgaaaaa accatagata
     2821 taaaaaatgc tagataagtt cttatgttac tcttaaaatt attataagca attccaagta
     2881 tactcactta catctcacct cccatagagg ctagcatatc cataatcttt ccataaaatt
     2941 cttttctact atttcctttg tttagtttac actttatatt tccatcattt agcatatata
     3001 cttcatttga atagcttgcc gttagagggt catgtgtaat cattataata gtagcgttgt
     3061 tttctctgtt tattttttct aatgattcta aaagttcata tgctgattta gagtctaaag
     3121 cacctgttgg ttcatcagca aatatgactg ctggatttgt aattaatgac ctagctgctg
     3181 cacctctttg tttttgtcct cctgataact gatatggata tttatctaaa tgattttcta
     3241 atccaaattt tttggctata gaaaatacct tagcttctat ctccttactt ttcttttttc
     3301 ctaatgctag aggaagtgct atattgtcca ttagtttcat attgtctaat agattatagt
     3361 cttgaaatat aaaccctatt ttttctcttc ttaattttga tagttcatta ttttttactt
     3421 ttgatatatt aattccatct agtattattt ctcctgaagt ttgtttgtca agtgtagaca
     3481 ttaaatttag taatgtggtc tttcctgcac cagatggacc cattatcgct ataaaatcac
     3541 cttcattgac tgtaagagat atatttttta acacatttgt tttaaatcct tttattccat
     3601 aatcttttga tatatttttt atttttaata tttctttcat aataatagcc tccagttgct
     3661 aattttttaa tatatcttta ttttaaaggt tttatataaa taaatcctta agtaaatatt
     3721 acattttaac tgttagcatt acacttttgt aacatatgga agactattat aattatagtg
     3781 gaaatatctt aaaataaaag catgattcac ttttttgtat aaacaaagaa aagaagccac
     3841 taagaggcct ctaatctata caataattct tctctataaa aaaacaaaaa tacaaccttg
     3901 attgtggtaa atacaggtac tataaacaaa tgatattacc actatatatt aaaacaatta
     3961 ttcctgaaga taatttagtg agactatcaa gccataagct aagcatccaa aaagacttaa
     4021 taccattaat gtataattat aaccaatatt aacgctcaaa aaaccagcaa cagttggaat
     4081 aatcagagat gagcctccca taaatatact taaagctatt aattggctac tacttctaag
     4141 ttttttagga gaaacttcaa atattaatct ttttgaagta attaaaatta aaggagtaga
     4201 aaacacttga aaaacacaca gtataataat gatatttgaa tcatttgtta aagcaaataa
     4261 tagaaactga attgttatca taatagcact taatttcaat aagtttaaac tgctatattt
     4321 ttttaataaa taagaaccag ccagatatgt tggtatctct aataatgact gtaaagacca
     4381 ctttaaagat atttgagaat ttgtagcatt taaagctatc attttatcta ttacagtaca
     4441 gttatttgct accaccattg agtataataa aaataaaata catactagta agacatattt
     4501 tttatctttt aataattcaa taatatcttt actagttaca tttattttct tattaatctt
     4561 ttcaatatca ggtaataata gtatatttat aataagtaat actacaatta aaccaatagc
     4621 tacagataat ccagaatatg agaatctaag aatcaagtaa gatgctacca tacttccaat
     4681 agcccatcca atcgacccaa aagtcttaat aaatgacaaa taattattaa tcttatctcc
     4741 acaacctatt atccatgtat catataaact acaacaagta tttaataacc ctcctgacaa
     4801 agcaacaagt aataagtata ttataaaagt tgcagattca gtagaaaaca taataactga
     4861 tgcaattcca taaactgata aactaattaa aacaatcttt ttcattgttt gatacttatc
     4921 agctaatact ccaaataata attgaaataa tatattggtt attgcatagc tcgataatat
     4981 aattcctctt tcaaatgctg agtaacctaa ttttgataag aaaggtgtga attttgttga
     5041 cgcaaaggca aaaataatat atgagaaaaa atagaatatg ataaatttaa atttaatcgc
     5101 tttattaatc ataatcaatc aaaattgata actttaatac cactatttcc ttcctgattt
     5161 aagtttgaaa gatgatattt taccgtatta ggattctcta caataactaa aggaatatat
     5221 aaagtatttt caagcaactt attatcaatt tctactaact tttcccccat attaacttct
     5281 tgaccttgtt taacttctaa tttaaagttt tctttttttg ttgttgcact atccaatcca
     5341 atatgaataa gaatttggac atcattttgc aatttaattc ctatcgcatg gttagtttga
     5401 gcaataaaac ttattactcc tttagtaggt gatttaagaa tattgtcctt tgctatcata
     5461 gcaaatccgt ctcccattac cttagttgaa aatactttat caggaatttc ttcaagatta
     5521 attatttttc catcaatgta ggaattaatt gtcattactt ctttttttct tttaaagaac
     5581 attgtaaaca ctcctctaca taattaactt tattctaatt atcattacaa ataacgatat
     5641 taagcatact gcaattgagg aaaaaagagc cttatagctc tttccccatt gtagttcttt
     5701 aatactgctt atgatttgtt taattccaac aaatttataa atataggtaa acatgataac
     5761 aatagttatt gtgtaaattt ggaatatctg ccctaataag actaaataga aagcaatcat
     5821 gttttctttt atctctttat tcttttttat attataaata acatatgcac tatatactgc
     5881 actaaagtta gagattaaat ttaaacttgc attaacatca ttgtttaaaa gttgtgaaaa
     5941 tgaaatgttt gtaaataatg atgtcaattc taaatacact atatataaaa caggtaatat
     6001 tagaaaacaa atcatcacca ctgaaagaaa tatattttca tcttttaata ctgctgtgat
     6061 attttttatt ttatttgtta tcatattttt gttctcctta tttaaaagtt ggccagaaat
     6121 ccttattagc cacaatcatt tcatctaaga tttcttttgc tacttttgca cttggaacag
     6181 ttttactcat agtaaacgac tgccataatt tttgataact tttttctatc catgcttcta
     6241 cagctaattt ttcagaagca acttgttgtt ccattaaacc tttttggaaa gttggaattt
     6301 tacctataga cataggttca actatatctt tcccaacaag gcaaggaatt tcaaccattg
     6361 catcatctgc aaaattttca atagctccat tattaggaac aatcagtaaa aatctttcct
     6421 gagtattaaa tgctaatgca caagctaatt caacaataaa ttgagaatgt tcacttgcat
     6481 caaaccatgt atctcttgct gtgccctttt ttataattct ttcacattct tcgaatgtat
     6541 ccttttctct tctatccata atttcatttg ctcttgtata atttggatta gcatgttcaa
     6601 cagtttcatc agccattaaa taatatttta aataagtatt aggtaatgta gctggttcta
     6661 tttctaataa gtcttttgcg gctctcatag tttctagcca actagattct ttgtgttgac
     6721 cttgaggtgt aaattcctca tatccttttt tagcacaata ttcctgtaat ttaggcatta
     6781 aatcatttcc ttctttgtcc ttaatacttg tccaccaccc gaaatgattc aatccatagt
     6841 atctaacatc aaagtcttta cgagaattta aacctaaaac tctagcaaat atttcctcta
     6901 aacatactgg catatcacaa atattaataa ttcttgaatt tggtcttaat acacgacatg
     6961 cttctgcaac tatagctgct ggatttgagt aattaagcat ccaagcattt ggagaatact
     7021 tttccatata atcaatcatt tcgattattg gacctattgt tcttaatcca taagcaattc
     7081 cacctgctcc acaagtttct tgaccaacaa ccccatattt tagtggaatt ttttcatctt
     7141 gttcacgtaa agcatataaa ccaacacgaa tttgtgccat tacaaaatca atatcagtaa
     7201 atgcttcttc tggatttgtt gtataactaa atttaatttc tggatttttc tctctaacaa
     7261 ttattgcaca tgcttcagca acttttcttt gtctctcatc attattatca tataatttaa
     7321 ttgattcaat aggaaattta tcgacacttt caattaacat tgttacaatt ccagcagtat
     7381 aagtacttcc ccctccagca attagtattc tctgtctttt tctttccatc tttccttctc
     7441 ctttataata aattttcaaa ttcttctctt acacttggta ctgaaagacc aataataatc
     7501 tgaatagcct ttccattatg cactaaacca tgtgcaccta ctgccttaaa atcttcttct
     7561 ttagaaataa ttgagtcatc tttcaaggtt aatctcaacc tcgtagcaca gtttgtaaca
     7621 tctacaacat tttctgctcc accaactaat tttatgtatt cagatgcttg ttgagaggct
     7681 ttactcaatt tatttccttt ttcatcttta gtttctaagt actcattttt agtatatagt
     7741 tttgtttctt cactatcttc ctctctacca ggagttttca tatcaaactt cttaattaag
     7801 aaagcaaata caaagaacca gataaatgaa aatataatcc ctacaacaac ttgaacaaca
     7861 tataccttcc agtgatacat tccaagagga agccaattta atacaaccca gttaataatt
     7921 cctccaccaa aatcaccaac aactcctaag gcaaacgaaa tcgtttgcaa gcacgcatct
     7981 aaaaatgcat gtactaggaa taaaagtggt gctacaaata aaaatgtaaa ttctaacggc
     8041 tcagtaattc cagttaatac tgcagtgaga gtagcaggaa tcattaaacc taatactttt
     8101 ttcttttttt ctggctttgc agttacatag aaagcaccaa atacacctaa tgaaccaaac
     8161 accttcgata atccggataa tgaaaatcca attggataca tttcctttag agtcttagct
     8221 gaagtttgaa agtcctgaat atgagtagcc caatatactg atgtaccacc tggaacaaca
     8281 acactatcat aacaaattgg tgcatataca aaatgatgta atccagttgg tattaaaatc
     8341 ttttgtaaga acgcataaca ccaaactcct atatttccac tatttataaa aaacgtttgc
     8401 aaatgtctaa taacattttg aatatgaggc caaataaaac aagttaataa tgcaactggt
     8461 atcataacaa agaaacaaat tgcaactaca agtgatgagc ctctaaataa tccaataaaa
     8521 tctggtagtt ctttatcata aaatctatta tgaacataaa tgacaatacc tgatattaac
     8581 aaagcaccaa ccataccagt atctaatgtt ttaataccag caatgcttac aagtccactt
     8641 ccagcactta tttcagctgc gaaatcaact ccaaagcttt ttccccaaaa acttaacata
     8701 gaacctacaa aataattaaa agtcaaatac gttgctaaag cctccataca agctcttgct
     8761 tgttgctttt ttgcaagtgc tattggtaaa gaaatagcaa ataataatgg taattgatta
     8821 aataccatcc aagctccact agatattaca ccccaaaatt ggtaccaaat attttctggt
     8881 gtagcaagtg aacccataat tgactgattt tgaaatacaa ttgagaatcc aaccattatc
     8941 ccagcaaaag caaataatag tgttggtgta aacattgccc ctccaaaacg ttgtattttc
     9001 ttcatcatat aaattactcc cctcctcata tttttagttt atctttggcc ttagatatct
     9061 attattttaa caggttaata aatatataca atctttaggg tacattcgga gcttattcac
     9121 agtcaaataa gtttttgcac atttttatgt tcaaattctc ataaaaaaaa gaatgactac
     9181 aatttgcaat caatcccttc tgtaatcatt ctatgccttt tataaatact atatttaata
     9241 aataataact caatcaatat aaaatataac attgtcaatt tataactagg gtgcatttgt
     9301 aaaaatgaga tattagtcgc tgaaatataa attgattcat cacttaaatc agtcaatgta
     9361 ttatttttaa actctgtaat tgaaattatt ttagttccta ttaattttaa tcttctagca
     9421 atgttaagtg tattctcatt ctcaccactt agggatataa taatcataac atcattttta
     9481 ctgacaatat tgtaaaaaga atcatcaaat gttcttccct caaaatcata tattatttct
     9541 tgacatgata agaacattct ctttatctgt tttgcaacca cattttgaat atcacctgaa
     9601 ccataagcgt atattgttct tgattcataa agaagtttac acaattcatc ataatcttta
     9661 tttttttgat cttcaacata cttaattatt gaattgcatg caatatcaaa tgactgattt
     9721 tctataacat gaaccttttc tgattcccag cttaataata ctttaaactc actgaaacct
     9781 tttaaatcta attttttaac aaatcttgta atagttgcac gagacacatt acatttcata
     9841 gcaagttcat ttattgacat cttactacaa tgttttttat ttgcagttat atatttccat
     9901 attaataaat ctgtatcatt caatttatta taattaaaaa aaatcatttc ttctaatctc
     9961 ataaactatt atccccttta attaaaaatc gtcataaata ttataccaat attcaacatt
    10021 aaattacagc atattcttat atttactatt tatcccataa aattgactat aaaaaatact
    10081 gctataattg atgtttaaaa aatgtagttt taattctatt cttttatcta ataattaata
    10141 ttttcaagtt ctagtgcttt tcttatgcaa ttatcctctt taaaatcagc agtcctttta
    10201 caatctttta ctttaaattc tggagtagtt ttaaattcct catcacatac tccactctta
    10261 tttaaataca tacaacttgc cattctaact attagaccac tattttttaa attaaacaat
    10321 acagggtcta tacaccctcc atctccacca gtagtttcac caactaaagt tgcaaattta
    10381 gtttctttac aaaacattgc aaatgactct gatgatgaat atatttttct atcaacaagt
    10441 aagtatatat tacccctaaa ttttaaatca tctgtagatt ttatagtata ggaagtatct
    10501 tcaaaaccat taaaatcttt tataatttct ttaggtgcat tttcctttac atttgctggt
    10561 aaactttcta caggatttaa ttttatattt cttgcatttg tatatttttt tactatttca
    10621 ttattatttc tataaattct ataaccattt attttgacat cattttttat gagtttagat
    10681 acgattccct tccaatacct atcacttcca cctaaattac ctcttatatc aataattaaa
    10741 gctttatgtt tttctaaagt gttgatataa tctccaatta tttttaaatc attatttata
    10801 gacccattct tcgatgacat tgatggtaaa tacatatatc caacctgacc cctaataaca
    10861 tcctttaaaa tcaattcttt ttttacaaaa atatcatctg gcatttttat tttaggattc
    10921 atagagtcat atctatctac aactttttta tcattaaaaa aatcgtacca atcattatta
    10981 gagtatgatt tcttaaaaag ttcatatctt ttcttattat ctattacttc tgtgtgttta
    11041 ttatttaaat cagataatat agatgacagt tcttctataa attcttcatc attttcagtg
    11101 ttttttattc gttttgtata ttcatcctta tttgctaacc aatcaatatt attaagcctc
    11161 ttatttacac ctagatatgg atatcctgtc tttacaacat tatacacata attaaagtca
    11221 tctacctttt cctcttcaga caactgattc ttttgagaat aagtagaatt acctaataag
    11281 attataaatg ctaaaataat tactaaaaat aatactttct ttttcatgta attctcatcc
    11341 actttcagat tttatatttt aatacattgt atcattattt tattatcacc taaacaacta
    11401 aaagaaattc ttgaagatat attccattat tattctcttt agaaaatttt tatttgtatt
    11461 ttcaaaatta tttcatgaaa gttataaaat aacaatcata ttcaaatatc tattttactg
    11521 ccatatcctg aaagtaaatt cttatcagtg atatactaaa aaagatatgc taaaaaattt
    11581 atccattata atgacttaaa tttagatact ttactgattt ttatttatta gttgagaaag
    11641 atattgataa ttaaaaatat tgataactat agaaatgatt attttttata gaaattttaa
    11701 ggatataaac taattatact taagtctata tccttaattt atcatgatta tgagaatttt
    11761 tacaaatact tatctttgtc cgttttagaa taaaagcaag aatatatcct gttattttat
    11821 tcaaacatcc aagtttaatt actacacaat tacttgttag aaattgtatc tacttcatat
    11881 atatatctgt taagtatagc ttctagcatt tcttgtcttc cagacttatt ctttataact
    11941 ggcatttgca gtgcataaga ttctaactct ttaaatccaa ccttaccttc tactatgtct
    12001 ttacctatac cttcactaaa acttgcatat ctttctttag taaagttttc aaatacctca
    12061 tcttctaata atttatgagc tattaataat ccctttgcaa atgtatccat tcctgcaata
    12121 taagctagga ataagtcttc tacctcaaaa gatgctcttc ttactttaga gtcaaaatta
    12181 aatcctcctg gtgctattcc accttgcttt aacacttcat acatagcaag tgttgcatca
    12241 tatatatttg ttggaaattg gtctgtatcc caacctaata ataggtctcc ttgatttgca
    12301 tctatactac ctaaaacatt gtttattctc gctatattta attcatgttg gaaagtatgt
    12361 cctgcaagtg ttgcatggtt tgcttctata ttcactttga agtatttatc cagattatac
    12421 tttcttaaaa atcctaaaac agtagcagta tcaaaatcat attgatgttt agtaggctct
    12481 tttggcttag gctctattaa gaattgtcca gtaaatccta tttccttagc ataatctaca
    12541 gccatttgta aaaatcttgc aaagttatcc atttcaagtt ctgtattagt gttaagcaaa
    12601 gtttcataac cttctcttcc accccagaat acaaaatttt ctcctcctaa gtacttagtt
    12661 atttccatag cttttttaac ctgagctgct gcatatgcat atacatctgc attacatgtt
    12721 gtagatgcac catgaacaaa tcttggattt ccaaacatat tggcagttcc ccataataac
    12781 tttatacctg tcttttccat attagctttt atatactcaa ctatttcatc tagtatctta
    12841 tttgtctctt ctaaatctct tccttctgga gctatatctc tgtcatgaaa acagaaatac
    12901 tcaattccca tcttagacat aaattcaaac cctgcatcaa ctttaatctt tgctatctca
    12961 atcgggtctg ttatattatt ccaaggtctc tccactgtgc ctactccaaa tgggtcatta
    13021 ccctctccac ataaagtatg ccaatagctc attgcaaatc ttaagtgttc tttcatagtc
    13081 ttattaccta ccacttgttc aggattgtag tatctaaatg ctaactcatt gtcagatttt
    13141 actccttcaa attttatctg tcctattcct ttaaatattt cactcatttc atgtcctcct
    13201 aatattttac acatataata tatgtagtat gttttcttaa tttaaaacta ttttctatct
    13261 ttatttttct ttttaagtta ttttaattat ttatttttgc aattaactac ttcatctaat
    13321 aacctaaagt catctttcaa agatttgtat attcttttat acaatctatg atatatcttg
    13381 tatgtatcta cattttcact tactggttct aatttagttg aagtttttat tatagcatca
    13441 caagcttttt gtatattttc atatattcca gctcctacta atgcaagaat tgcaactcca
    13501 agtgctggtc cttcacttga atttatagta tatacatctt gattaaacat atctgcttgc
    13561 atctgtctcc atactttact ttttccacct ccacctgagg ctcttacttc attaacgtct
    13621 atattaagat tttctattat atccatacag tctttaagac tatacgaaac accttccatt
    13681 attgaccttg cgatttcttt tttagaatgg attggagata atccaaagaa cacacctctt
    13741 gcataagggt caagatgagg agttctttct cccatcatat atggtaagta aatcaatcca
    13801 ttacatcctg ttggtacttg acttacttga ttatttataa tctcatatac atcttcacct
    13861 aaagaatttg aaacttccac ttcactttga cagaaattat ctttaaacca ttttaatgac
    13921 aatccagctc cttgagtaac tcccataaca tgccatgtat ttggtattgc atgacagaaa
    13981 gtatgaactc taccttcttt atcaattttt ggctcttttg tataagcaaa tactacacct
    14041 gatgtaccta tagtagatga aactactcca tctttaacta ttccatttcc tatagctcct
    14101 gctgcttggt ctccagctcc tcctaccact attgttcctt ctttaagccc tgtcaaactt
    14161 gctacatctc tagtcacata acctgtaatc tcttgtgatt cataaacttt acctaaaaga
    14221 tttttatcta tatttaactt ttctagtact tcatcgctcc aatctctttt tcttatatcg
    14281 agcaactgca taccacttgc atctgatacc tctgtagcat attcacctgt caatttgtat
    14341 cttatataat cttttggaag aagtatttta ttagtatttt tatatatatc tggttcattg
    14401 tttctaaccc ataatatctt tgatgctgta aatcctgtaa gtgctggatt agcagttatt
    14461 tcaatcaatc tttcttcacc tactaaatca gttatctcct cacactcttt tactgttcgt
    14521 tggtcacacc atattataga gtttctcaat gttttacctt cattgtccag cataactaga
    14581 ccatgcatct gtcctgataa tccaattcct tttatacatt cactgcttac atttgaactt
    14641 tgaactactt ctcttataga ttcgactaca gctttccacc aatcatttgc atcctgttct
    14701 gcccaacctg atttctcttg tataagagga tattcatatg tacaactttt tatagtattt
    14761 cctagtttat caaataaaac tgttttagta cctgatgtac caatatccac acctaataca
    14821 tactccaact tcatccctcc ttgaatgcca cttcataact atcgttttcc tatcctaatt
    14881 tttttatatt ttaggtatat tttacttgct ttatttttgt tagtatatta aactaacttg
    14941 tgaactaata ataaaatatt taattatctt tgtcaatgca aaataacctt ttatttttgt
    15001 gtaaaaagta ttacaatatt attttaagga ggaatatcaa attggttact gataaataca
    15061 ctattcgaga aatgaatgaa agattggtac ttgaacaaat tataaaaaat ggtcctattt
    15121 ctagggcaag tatagcttct actataggat tgaataaagc aactatatct gctattacca
    15181 aaaaacttat tgatgaatct ctagttcatg aaataggaat tggaaatagt actcatagtg
    15241 gcggaagaaa acccatatta ttagtattta ataaatgtgc agggatttct ttaagtatgg
    15301 acattggata cgactatata ttttcttctc tatcttactt agatggaact attataaatt
    15361 ctaagaaatt aacagatata caagtaagta aagacaatgt tatacaattg atagatgaaa
    15421 ttataaatag ttataacata tccaaaatag acactcctta taaggtaatt ggtcttactt
    15481 tggctataca tgggattact tgtgaaaata aagtattgtt tactccatat tacaatctta
    15541 atgagattga cttatatagc atccttagca aaaagtatga ctttcctatt catatagaaa
    15601 atgaagcaaa cttaacagct ttagctgaga atactttttc aactgtacat aactcactac
    15661 ttagtctcag tattcacagt ggatttggtt caggcattat aataaataat aaattatata
    15721 gtggcagaaa tggtatgagt ggagaaattg gtcacactat catcatgcct aatggtaaac
    15781 tatgtccttg tggaaaccgt ggatgtttag aacagtattg ttcagaaaaa aaggtatttg
    15841 aacagctttc ttctttagaa aatattccta aaatagattc tgatatagta aagcaactat
    15901 attatgaaga taaccagaat gccaaaaaag ttattcatga gttttgtagt tacttaacaa
    15961 tagctataaa taatgctatt acaacttatg ctcctgaaat aatatattta aatagccaaa
    16021 taattagtga tatccctgag atattacaga ttacaaaaga tatgttagtt agctctttta
    16081 acaaaggaat aaatatagaa atttcatcat tagggtctga ggcatctcta tatggtggct
    16141 ctgccgtaaa tatcaagagc tttttgaata tacaaaattt aactttaatt aatgaaatat
    16201 aagggttatg actatataca gccataaccc tttaaattat acattctaaa attagattta
    16261 ttatatagtt tatgcaagat tagctatatt ttttaaaaat tgtatatcaa ttaagtatta
    16321 acacaagtat tttgttttaa atattaatat aatcttccta ctcatcatca gataataaac
    16381 ttgcattttg ttcaaccata gaaatattat ctttacctat tttctctaat tttgctgcca
    16441 attcctttgt atctaaaaaa gctgattcaa taaccatacc taaatttagc cctgaaataa
    16501 ttttaattcg actgtcatta ttggctacta ataaagcagc attgcatgga gttccaccta
    16561 gtaaatctgc cattataatt atttctgaac tttccttgta cacttctaaa gcttttttta
    16621 atttttctat tgttccttct attccatcat cttgtttcat atttacagta ggataatcta
    16681 tagaatcccc tattatcatt tttgcagaat ttaagacttc ttcagccata ttgccatgac
    16741 tcatcaaaat aatactattc actttaatac acctctttaa atctataaga tgtctacaag
    16801 ataagttttc ttatcatcag gcgtcattat agttgtaatt tcaattcctt catccaaaag
    16861 tcttttaaat gcttcttttt cttcttcagt aacactgata ttaggtttta gcactgttgt
    16921 gtcttttcta cttgacatat ttcctacatt tatactgtgt attttaagtc ctgcatctac
    16981 aaatggaata aggtccacag gagacttaac aattaataat acattttgcc cttcatactt
    17041 tcctgcagta atatttttta tagcagtctc ttctgtaatt atagatgacg ctatacctgc
    17101 aggtgtagcc attcttaaaa cttgtttttg aacagaattg ttagctactg catcatttac
    17161 taccattaat cttgtaacac ccaatttatt actccacata gttgcaactt gaccatggat
    17221 taaacggtca tctatacgaa tatttttaat acctttcatt tccatatcta atatcatcct
    17281 ttccttattt taaaatacct aatgcatata aaattattga aacaaccatt actcctaaaa
    17341 ttaagtatat aggttttata ttctttttaa gagcccaata aacaatcaca actaaaattg
    17401 caggaactaa tccaggcata atttggtcta atatttcttg acctttcatt gtaaaatcac
    17461 cttgttgaaa gttcaaagca acattagctt ttacaactga aggtattaat gcacctacaa
    17521 ccattaatcc taaaatagat gtactctcag ttaaaatctt taaacttttt tcaattgaag
    17581 atattagctt agtacctgat gtataaccag cattaaataa tggtatactt aataatttca
    17641 atgctacact cactacaatc cacagtaaga ttccaaatgg acttccatct atcgccatat
    17701 tagctgctat agctccaaaa attgtccacg gaataacgac aaataaacta tctcctaaac
    17761 ctgctactgg tcccatcaag cctgttttaa ttgaagcaac agcctcttca gtctcttcac
    17821 ttgataattc ctccattgct acattaatac ccaaaatata aggggcaacc caaggattag
    17881 tgttaaaaaa ttgtaaatga tttaatacag cactttgtaa cttttctgga ttatctttgt
    17941 atactacttt taaaaaaggt agtactgcat aacagtatgc caaggcctgc atcttctcat
    18001 aatttgaaga aacagaacta gtaaaaaacc agcgccataa gacacttttt ttcacctttt
    18061 catttgattt atttaatgat accttatcac tcatcttcgt ccatacctcc tactacagta
    18121 tattgttgtt cttgattttc caaattcttt ttgtaaatta taagtgcaat tgctccacca
    18181 attaaagcta cacctaataa tggtactttc atatatactg ctaaaacaaa accaattact
    18241 aagtaactaa aatactcctt agcaggaaga tatctaagta ataatcctat ccctactgct
    18301 ggaagtagtc ctccagctac tgttaaacca cctgttaacc attcaggtgt atatttcaaa
    18361 atggtattaa ttaaagaagg tccgaatatt atagatagag caactggaat acctgttgta
    18421 gctgctgtta atagtactcc taacatttgc atataggtta tatttttata atttcctttt
    18481 ttagctccat cttctgcttt atgaactagc catataccaa tagtatttct aagaacatct
    18541 aattgtacca tcaataaagc aactggaata cctatagaga ttcctacaga tgcttcttgc
    18601 ttagtcgtaa tagttataaa tgtggctact atacttgctg tttgataatc aggtattgat
    18661 gctccaccaa agttactaat acctaatgat aatagttgta atgtacctcc aatatataac
    18721 cctgtattta tatcacctaa gataagacca gtaataaaac ctgctgttac tggttgatat
    18781 gttccaaaca ttgttgaatt tttttcataa tttataaaaa atccatatag tacgattaat
    18841 aataattgcc ataattgtgg ctccattatg atttcacctc tcccaaaata ttttttagtt
    18901 ctttaaaatt ataactggaa tcttgcttta ggaaaatagg aatttcttcc agtgggcatt
    18961 caacttcata agtcttccca ccttcatagc taactccact aaatatattt tcccatatat
    19021 gtccttttgg taaatatact tttctacttc tatctttata attcattatt ggagctacca
    19081 aaatttcatc tccaagcata tatgtattat ccacctccca agttttatca tcttcaggaa
    19141 attcataaaa taaagttctc ataactggtg ttccgtcttc atgcgcttct ttcatcagtt
    19201 tttttaaata agtttttaag ctctctctaa tttttatgaa ttttgtaaga attacttcaa
    19261 cttcttcacc ataagaccat atttcattag gagctccagt taccattgag cctccacctt
    19321 tatttgataa tggtttacta tgaggcaatc tatctccatg cattctcaaa attggtgaaa
    19381 aagtagcata ttggaaccat cttaccataa gttctctaaa ttctgggtct ttaggatttc
    19441 ccccatggaa acctccaata tcagtagtcc accatggtat acctgccagt cccatattta
    19501 accctgtatt tacttgatta ttaaaagcct caaaactaga atcaatgtca ccagaccata
    19561 ctaaagcccc atacttttga gctcctgccc aagctcctct tacaagagtt acaacagact
    19621 ctatgtcatt atgtaaacca tcataaatca tttttaaata atcaatagga taaatattac
    19681 cacaacttaa aacatttcct tttttatatc tatagttatc aaaatcatac acagaatagc
    19741 caggttcagc tacatctaac caataatagt caatcccaag ttgcttataa tttttatcta
    19801 ttctatccca aacatattct cttgcatttt catttgtcat atctacaaag actgtatttc
    19861 cttgaatttg cattgtcatc ctaacaccac gattaacatt gactaaataa ccattttcta
    19921 aataatcatt gtagttttct gcatcacttt gaactgtagg ccatacagat actattggtt
    19981 ctactgaata ctcttctttc aattttttaa ctaattcttt aggatttttc caataatcta
    20041 agtcaaattt atattcacct tgcttaggcc aatgaaaata gtcaattgca attgatgaaa
    20101 gcttaattcc tcttttagaa tattccttca caacatctaa aacttcttct gaagttctat
    20161 atcttagttt actttgccat aatcctaaaa ggttttcagg catcatagga acagttcctg
    20221 taacttgaga ataattttta tttaactctt ttgggctttc tccacaagta atccaataat
    20281 ctataaaatt tgtagaaaac atcttccatt ctgtcatatt tttagcaaaa gatacttttc
    20341 caattcccgg attattccat aaaagtccat aacctagact agaaatataa aagggaactg
    20401 atatttgaga atttctttgg gctaactcca aaacagtatt ctttaaatct aaaaatttat
    20461 gttgatattg tcccattcca aaaatctttt cggaaggctc agattcaaat ctagtagtta
    20521 cttcaaattc tccactatag cttggtcgat attctcttga ttttaacttt aaagtagagt
    20581 taaagtcttt ggtaatctca attgtcccta catcttctcc accatcatca tgttttacag
    20641 ctcttaatcg aatatattct ttcaataata tttcattttt atcattataa aaagtaattc
    20701 tatctctatg gtctaagaca gcttttatct ttccattttt aatgattgca cttccatctt
    20761 catttttatt aattgtaata tcatctttat ttaacttagg tttttcgtta agagcatagt
    20821 tttcatcaac aaaattttgg tctacaaacg acctaatacg taaagagttt tttccccatg
    20881 cttctattcg caatagttca ttgtcaaatc ttttttcaat atagtcttca gatattttta
    20941 tcacggctac ctcctaaatc aatggtataa tattctatct ttgtgtagct taagttaaat
    21001 aatgtcgttt agtatcgtta tttataaatt aggttttata tataattcat ttgtacatta
    21061 aactaactta ccaataatat actttatttg actttgtttg tcaatcgctt tccggatttt
    21121 agtaaaattt tctaatatat tgatggaaag tcattcttaa tattttttat ttatactttt
    21181 aatatctgtg atgataccta tatacatcta gattttttaa aacacatatg ggaataacgt
    21241 ttgcctatta tgtagataat atcgtacttg aaaatctgta tagtgacctt ataaatacag
    21301 ataataatat attagatatt attcaaaaca atggatttta taattataaa taatttatga
//
LOCUS       DAEPXF010000060        21106 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain HB27
            SAMN13376389-rid12479423.denovo.064, whole genome shotgun sequence.
ACCESSION   DAEPXF010000060 DAEPXF010000000
VERSION     DAEPXF010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13376389
            Sequence Read Archive: SRR10518432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 21106)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21106)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001069624.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001069624.1
            Genome Coverage       :: 315x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/15/2021 01:54:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,165
            CDSs (total)                      :: 4,076
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 89
            rRNAs                             :: 10, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 10 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 19 of 69
            Pseudo Genes (incomplete)         :: 44 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 10 of 69
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21106
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13376389-rid12479423.denovo.064"
                     /strain="HB27"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="China: Hebei"
                     /collection_date="2017"
     gene            complement(121..1128)
                     /locus_tag="KRL67_001864"
     CDS             complement(121..1128)
                     /locus_tag="KRL67_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase S55"
                     /protein_id="HBH1522744.1"
                     /translation="MQLKNFHFKNNTLSKKTNLAKSKFTIVLTFLFLLYFFSNNLIYA
                     QNLQKNEPEYLIPIGNVLQIDAELKNIIVRNPGEASPFRLGDAIVKVNNAEVDGYSDF
                     ANTLNALPEEKQVSILLNRGGQLITMKTTKHILEKISFNNLLSGFATLTYINPDTSEF
                     GAVGHPISVGNARKIPIKTGSISTTKHLNIEKSYKGSVGCINAKRENIIGSFTENTDF
                     GIRGQINNFDISKLKKYKVASLDEVKLGKAQIILQDNFNKCKKYNIEIIDIENQKHPE
                     SKSFKIKITDKKLLAQTGGIVQGMSGTPIVQDNKIIGAVSHAIENDPAVGYGVFIKWM
                     L"
     gene            1356..2240
                     /locus_tag="KRL67_001865"
     CDS             1356..2240
                     /locus_tag="KRL67_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HBH1522745.1"
                     /translation="MKKYRLLVTCIIAMGIIIIGTSNPVSNFYKTTNLISSGNITNEK
                     ADKGENSNKNNKDKNEDKSNSKSNKDKSSEDKSSDNKNEIQKNKKLLICIDPGHQGKG
                     DSNLEPVAPGSSSKKARVSSGTEGIATKKPEYVLNLEASLVLKSILESKGYNVIMTRE
                     THDVNISNSERAILANDKKADMVVRIHADSLNNSSKTGASILIPEKDGKYTAPIYEES
                     NKCAEFIKQNMEQSGIQINGIVQRGDLTGFNWSKVPAVLVEMGFMSNYNEDQMMSNPD
                     YQRKMMQCIADGLDEYFK"
     gene            2442..3992
                     /locus_tag="KRL67_001866"
     CDS             2442..3992
                     /locus_tag="KRL67_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018592108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HBH1522746.1"
                     /translation="MLVVENVSHGFGARTILENVSFRLRKGEHIALVGANGEGKSSFL
                     NIITKKLMPDAGNIKWSSRATVGYLDQHTVLSKGKTIREVLREAFKHMFDLEQEMIAM
                     YDKMGEASDDEMSNLLEETAEIQTILENSGFYMIDAKIQEVANGLGLGEIGLDKDVTD
                     LSGGQRTKVLLTKLLLENPTILILDEPTNYLDEEHITWLTKYLQEYENSFVLVSHDIE
                     FINNTCNVIYHMENGELNRYKGNYDEFVRLNDIKKRQEEQAYDKQVEERKRLEDFVAR
                     NKARVATRGMANSRQKQLDKMEILERPKEKIKPTFAFKDARAASKIIFETENLVLGYD
                     EALTKPLNFHLERGKKIALKGMNGIGKSTLLKTLLGIIKPFEGNVKLGDYLEVGYFEQ
                     ESSRENSNTPMDEVWSEFPGLTNFEVRQALAKCGLTNEHITSQMRVLSGGEAAKVRLC
                     KVMLKNINFLVLDEPTNHLDVEAKDELKKAIKEFKGTVLLVCHEPEFYSEIVDDVWNI
                     EDFTTKIV"
     gene            4021..4656
                     /locus_tag="KRL67_001867"
     CDS             4021..4656
                     /locus_tag="KRL67_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01906 family membrane protein"
                     /protein_id="HBH1522747.1"
                     /translation="MKKFLNVLFSVCISIVIIVGVINFTVGFKQLYYFDIDYLNISEL
                     SGLSKDDIKLNYDYLIDYNLNKNVSEFKLPTLKFSPQGKIHFEEVRNIFQNINKLAKL
                     LLVVSLVGIILSVKNKNIKILKTTSITLIIMPLLLTVPILLNFEKSFIIFHKLLFRND
                     YWVFNPNLDPVINILPEEFFFHSGMMILILILLASILLFVMYKLCKSFRIK"
     gene            complement(4767..5027)
                     /locus_tag="KRL67_001868"
     CDS             complement(4767..5027)
                     /locus_tag="KRL67_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich small domain-containing protein"
                     /protein_id="HBH1522748.1"
                     /translation="MGENYKFFNHKDCEFFPCHKTNKPEEFNCLFCYCPLYALGENCG
                     GNFKYTDKGIKDCSSCMLPHKKDNYNYIMGKFQDLVKITSKK"
     gene            5244..6527
                     /gene="hflX"
                     /locus_tag="KRL67_001869"
     CDS             5244..6527
                     /gene="hflX"
                     /locus_tag="KRL67_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HBH1522749.1"
                     /translation="MKEIQEKALLVGLNLTTIVKKNDDIDTSESMEELKELTKAAGAE
                     VVGSLIQNKHSVDAAYYIGKGKVEEIRAYSDSLDATLVIFNDELSGAQIRNIENVVGR
                     KVIDRTTLILDIFAQRALSKEGKLQVELAQLKYRLPRLYGMGGEMSRTGAGIGTRGPG
                     EQKLEIDKRHILNKAADIRRELKEVKKNRETQRVKRLKSNIPIVALVGYTNAGKSTLL
                     NELIKTHKDYEQEKEVFVKDMLFATLDVTLRKALLPNKKEFLVVDTVGFVSKLPHDLV
                     EAFKATLEEVQYADLILHVIDATNTSYELQKSTTEGVLKELGVNDKKHILVYNKVDKL
                     ELDIYPKSQEDIVYISAKQGINMDKLLNMIEIALMENTYSVSLMLPYERGDIFSRIKD
                     KYNVENFEYGESGITLDVDLDEEDFNIYREYILEK"
     gene            6535..7212
                     /locus_tag="KRL67_001870"
     CDS             6535..7212
                     /locus_tag="KRL67_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HBH1522750.1"
                     /translation="MDINWENIDNLEDYFITYLLYKESKTVSQISKIRNISSTEASEQ
                     LIQAKLKIKEMQKDDFEASKDILDKFLELDKIKRLDFMDSLDDEKMVYFKRKVFKRIL
                     VEKNAEDLIVLIWATGELKDDKFLKLLHQLTNHRHSDIRRITYSAIRKIESPSSREVL
                     QKGLYDKNAQTRQYCAKALSKLGDENSLKILQQLKDKNKNFEKEYVLRAYDEAIKSLE
                     NAKNLKK"
     gene            7298..7741
                     /locus_tag="KRL67_001871"
     CDS             7298..7741
                     /locus_tag="KRL67_001871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HBH1522751.1"
                     /translation="MIVRRCAGGVVFYANKVLIVKNDRGEWTLPKGKILGGGLPYESA
                     VQRVKVETGIDAKMIDVAGDTMYEFFSRSRQQEVCNAIMWYVMEACNTDCVLAPEFQE
                     GGFYKVKDALEMLSHHKEQALVEVSYKKFKELKKLVSEGDDVPAN"
     gene            8046..8690
                     /locus_tag="KRL67_001872"
     CDS             8046..8690
                     /locus_tag="KRL67_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HBH1522752.1"
                     /translation="MSNYRTLHEFGTDEITIEKSVFIGYAKPIKSEEEAVEFINEIKK
                     KHKDATHNVWAYTVGKNMNIQRYSDDGEPQGTAGIPTLEVIKKEDLRDVAVVVTRYFG
                     GTKLGAGGLVRAYTKGAKLGLEAGKIIYKVMYQEVKVKIDYTQLGKVQNELMNLGYFI
                     KDTVYEDNVEIVVYSRLEDVEKLSEKMIDITSGTGKIILGEEFYLSEQNGEILI"
     gene            complement(8776..9153)
                     /locus_tag="KRL67_001873"
     CDS             complement(8776..9153)
                     /locus_tag="KRL67_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HBH1522753.1"
                     /translation="MLFALIGFFAGIIGGMGMGGGTILIPALVLLAGIDTKIAQSVNL
                     LSSIPMTLIALAIHIKNKNVIFKLVIPIAVFGVLGAVFGSLLANYLSSDILKKIFGVF
                     LLLVGLFEVKKGFCAKSDKKPLN"
     gene            complement(9153..9539)
                     /locus_tag="KRL67_001874"
     CDS             complement(9153..9539)
                     /locus_tag="KRL67_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HBH1522754.1"
                     /translation="MKKTDDLKKKYFDFNLKNTIIGVFTGFINGVFGSGGGTLLVPIL
                     NDIVKVEEHKSHATALSIIIFLTTASSVLYVSKGTYDVNLTIKVAVGSILGGIIGAKL
                     LNKVTGKFLRISFGIIMIIAALRMVF"
     gene            9718..10473
                     /gene="nadE"
                     /locus_tag="KRL67_001875"
     CDS             9718..10473
                     /gene="nadE"
                     /locus_tag="KRL67_001875"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) synthase"
                     /protein_id="HBH1522755.1"
                     /translation="MSNIKIQIDKTVEWLINKVNEANAKGLIVGVSGGIDSAVVANLI
                     KKAFPENSMGVIMSIKSNPQDREDALKVIEGCDIEYLDLDLIEPQSAILDMVVGNLKD
                     KHLYREEYLKMTDANLRARVRMSTIYTIANNLGYLVVGTDNAAEIHTGYFTKFGDGGV
                     DILPIANLTKGEVYEWAKELGVHEDLINKAPSAGLWEGQTDEDEMGTTYNMIDAVLEG
                     RLDEVPKRDQEIIERLHRLSEHKRKTPAQPPKF"
     gene            10556..11275
                     /locus_tag="KRL67_001876"
     CDS             10556..11275
                     /locus_tag="KRL67_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBH1522756.1"
                     /translation="MGRIGNIINRKGKQDAQRAKIFTKHARAIAVAAKEGGADPEYNA
                     ALKTAIEKAKADNMPNDNIDRAIAKGAGAGAGEDYETIVYEGYGPGGVAVIVETLTDN
                     KNRTAGNVRYYFDKNGGNLGTSGCVSFMFDKKGQILVGLGDGVSEEELMDVALEAGAE
                     DFITEEDGYEIITTPEDFSSVRDELKAKGYEFISADVKMIPQTTTVLTEESHLKMMNK
                     LVDMLEEDDDVQDIYHNWEVE"
     gene            12051..12845
                     /locus_tag="KRL67_001877"
     CDS             12051..12845
                     /locus_tag="KRL67_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HBH1522757.1"
                     /translation="MESVMENLNNLKVEVKDKVCVITINRPKALNALNSDTLRELSQV
                     IDVVSENEAILGVIITGEGKVFVAGADIRQMQNYKSEEGRKYAGYAQGIFDKIEALEK
                     TVIAAVNGYALGGGCELAMSCDIRIASEKAIFGQPEVNLGVIPCFGGTQRLSRLVGTG
                     IAKELIFTGRQVNAEEAKSIGLINKVVPSDLLLEESMKMMNQIVEKAPIAIRYAKVVI
                     NKGIDMDLKNALELEKDIAGLTFATRDKQEGMNAFIEKRKPVFENK"
     gene            12939..14279
                     /locus_tag="KRL67_001878"
     CDS             12939..14279
                     /locus_tag="KRL67_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntP family permease"
                     /protein_id="HBH1522758.1"
                     /translation="MNTGVIISLVGIIIALSLLVVMVMKGINIFIIAIVCSSLVAITG
                     SLNVYDALKVDYMTGFVGFLQANFFIFLTGTLMGKIMEITGGAKSIAKMIVRWIGKDK
                     ALLSIPIACGILAYGGVSVFVVSFAVFPIALEIFKEADLPRRFIPAALTFGCSTFAMV
                     APGAPQIQNIVPASTLGTDIMAGAVNGFISCAVMFIVGSTILYKMVSKEKANGGHFIA
                     HESDIFDEAEATNSKRENGPNGVIALIPLIVSILIINVKVNGKAIVPIEVGVFIGALL
                     VYLLLNKYQDNTKIVGHVGDACKTTVVAICNTCAVVAFGSVVKSAVGFDFIVNAMTNI
                     PGPPIAGAAVGTTVIAGICGSASGGLGIAAPLLGPVFLAKGVSAAALTRTMAISSAAL
                     DSLPHNGYIVTVTNGLCKETHKDAYMPIFWVTVLTPFIGTIVAVLLFTMFPGLP"
     gene            14416..15648
                     /locus_tag="KRL67_001879"
     CDS             14416..15648
                     /locus_tag="KRL67_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HBH1522759.1"
                     /translation="MLNKPLEGVKVIDLTYFVAGPGTSKILADWGADVIKVEPSFGDP
                     GRKTGATMTMPIDDYNNPFYSTYNSNKRGLSINLKSETGIEIMDKLLSEANVFVSSYR
                     TGALKRLGLDYESLSKKHPHLIWGQINGFGDFGPAKDNAGFDTVAFWARSGAMLDIAE
                     KDTSPINPPIAFGDATTSCSLSGGICAALYQQAKTGKGQKVMASLFGQAIWNASSLLA
                     STQFSDEYPKTRKNAISPVINSYRCKDGKWIFLSILEHERYFEALCRLFGREDLIKEE
                     KFASSVESKKNATELINILDGEFAKFTQNEMVEKLTAADIAHEKIQHVNDVVTDEQAI
                     ANNYIYEHTSKNGNKTMLASTPVKFGNIEVNMTCDAPLIGEHSDEILRELGYSDSDIE
                     ALIESGIVTMNKEACEIC"
     gene            15688..16830
                     /locus_tag="KRL67_001880"
     CDS             15688..16830
                     /locus_tag="KRL67_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HBH1522760.1"
                     /translation="MDFRLTEAQLMLQRVAKEFAENEIAPIAAETDKTGIFPRELFSK
                     MAKIGFNGIGTPVEYSGSGGADIEKVIVVTEIAKKCAASAAILSIHTIYAQAILKFGT
                     EEQKKKYLPMMAEGGCVGAFALTEPNAGSDAARAATTAIIDEETDEYVLNGTKCFISG
                     GGQAESLIIFALTDPSKGIKGMSAIIVDKGTPGFSIGKIEEKMGIHGSETAELIFDNC
                     RVPKSNLLGKEGKGFNIAMTCLDGARIGVGAQAVGIAEGALEESIKYSKERVQFGKPI
                     SALQGIQWYIADMATMVESAKLLVYYAADLKARGEKHTKEAAMAKYNASRTAREVTNL
                     ALQIHGGYGYMKDYPLERMYRDAKITEIYEGTSEIHKVVISRAVLG"
     gene            16841..17635
                     /locus_tag="KRL67_001881"
     CDS             16841..17635
                     /locus_tag="KRL67_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009899720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HBH1522761.1"
                     /translation="MNIVVCLKQVPDTNEVKINKETGTLIRDGVPSIINPDDRNALEE
                     ALKMKDELGAVIKVISMGPPQAKSALKEALAMGADEAYLISDRAFGGSDTWATSTIIA
                     AAIEKVGKYDVIFCGRQAIDGDTAQVGPEVAEFLGIPQVTYAKEVKVQDDKLLVTRYT
                     ETGDYLIEAKMPVLLTAIKELNNPRYPSVKGILEAYNNGDAKITVLTLADLDVDTTQI
                     GLKGSPTNVYKSFVPVKDKHNEIIEGINKKEKAEKLIEILFDLKLV"
     gene            17646..18668
                     /locus_tag="KRL67_001882"
     CDS             17646..18668
                     /locus_tag="KRL67_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HBH1522762.1"
                     /translation="MMRAKVNQGINLNDYNGVWVIGEQREGKINPVTIELIGEGRKLA
                     DQLGKELAVVIAGYEVEKEVKELLHYSVDKVYYINDPLLKDFTTDGYAISIANLIERK
                     KPEVVLVGATSIGRDIAPRIAGKVGTGLTADCTKLEIDSTDNKLLQTRPAFGGNLMAT
                     IVCPKNRPQMSTVRPGVMAKAVRNESETGILEVVTPELTEKMIRTRLVEILPQEKKSV
                     NLTDARIIVSGGRGLKRAEGFELIKELADKLGAEIGASRAAVDSGWIEHSHQVGQTGT
                     TVRPELYIACGISGAIQHLAGMSDSKYIVAINKDAKAPIFSICDYGIVGDLYEIIPEM
                     IESLNR"
     gene            18767..20143
                     /locus_tag="KRL67_001883"
     CDS             18767..20143
                     /locus_tag="KRL67_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent Fis family transcriptional
                     regulator"
                     /protein_id="HBH1522763.1"
                     /translation="MKEQWYKDIFARVLSMTDDGFIVVNTSGVIIDINDKYCDFLGKE
                     RKDIIGQNIQSIIPNTKMLDVMKDKYCEEGAIHHYSGGNTKEKSVIVSRSYVENDNGE
                     VVAGVAQVKFRLQSFDVAKKLMSEYMELQYYKEQFKDNCGFDKLIGENRDFIELKKTG
                     VKASKTNFPVLLTGETGTGKEVFARAIHNNSSRSDKPMVSINCAAIPEELLESELFGY
                     DEGAFTGAKKGGKKGKFLVANNGTIFLDEIGDMPLTMQAKLLRVLQESEIEPVGGLKT
                     IKIDVRVISATRKNLSKMVEEGLFREDLYYRLNVINIHMMELKDRQDDILLLANYILN
                     KLNVEYKELKVLSDKVKNCFINYTWPGNIRELQNVIKSAYAVSDDMVIMMCDLPSKMD
                     NISRVAQCNVDSNCSIHEMVENYEKSLIIDVLRKYNWKCSKAAEVMGIHKSLLYKKIK
                     KYEIELNN"
     gene            complement(20473..20817)
                     /locus_tag="KRL67_001884"
     CDS             complement(20473..20817)
                     /locus_tag="KRL67_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH1522764.1"
                     /translation="MDQIKRLHELQQKSYLTKEEFEELKNNTYIERFELRDECDNSYI
                     YTFYTNDKCNDIKFIKSQFIVTLIKIGFLCECGGVFRQKKIVDEYDYGYNAIYKCDSC
                     GKELDKEVENYD"
BASE COUNT         8106 a         2403 c         3931 g         6666 t
ORIGIN      
        1 atattttata ctatgtaaaa tatctaatat atttggtctg taggttaaat aaaagtacct
       61 ttagaccaaa tatattatta ataacttaat aacatcttaa aacttattta tttatattat
      121 ttacaacatc catttaataa atacaccata acctacagct gggtcgtttt caattgcatg
      181 tgacactgct ccgattattt tattatcttg aactattgga gtaccactca taccttgtac
      241 aataccacca gtttgagcta aaagcttttt atctgtaatt ttaattttaa aacttttact
      301 ttctggatgt ttttggtttt ctatatctat aatttcaata ttatattttt tacacttatt
      361 aaagttatct tgtaaaatta tttgagcttt tcctaattta acttcatcta aagatgctac
      421 tttatatttt tttaacttgg aaatatcaaa gttattaatt tgacctctta ttccaaaatc
      481 agtattttct gtaaagctac caataatatt ttctcgtttt gcatttatac atccaacact
      541 acctttataa gatttttcta tattaaggtg cttagttgta gaaattgaac ctgttttaat
      601 aggtattttt cttgcattac caacacttat tggatgacct acagctccaa actctgatgt
      661 atctggattg atatacgtta aagtagcaaa tccagaaagt aaattgttaa aactaatttt
      721 ttctaatatg tgtttggtag ttttcattgt tataagttgt ccacctctat ttaataaaat
      781 agatacttgt ttttcttctg gtaaagcatt taaggtattg gcaaagtcac tatatccatc
      841 aacttcagca ttatttacct ttactatagc atcacccaat ctaaatggag atgcttctcc
      901 aggatttcta actatgatat tttttaattc tgcgtcaatt tgaagtacat ttccaattgg
      961 tattaaatat tcaggttcat ttttttgtaa attttgtgca taaattaaat tatttgagaa
     1021 aaaatataat aaaaataaaa atgttaaaac aattgtaaat tttgacttag ctaagttggt
     1081 ttttttactt aaagtattat ttttaaaatg aaaattttta agttgcataa aacacctctt
     1141 tcttattaaa ttattttaat aatagaattg aagttttctt gctgcttcta tattatgttg
     1201 tgcaagaatt acgttttgat gcttggaaaa ctgactcaat aacaccttag ttttcataaa
     1261 aataaataaa ataaatagtg tatatgataa aataaataca tattcatata aataaaaata
     1321 aacaataact gtctagcata agaaaggagc aaaaaatgaa aaaatataga ttgttagtta
     1381 cttgtataat tgccatggga atcataatta taggaacatc aaatccagtt agcaattttt
     1441 acaagactac aaatttgatt agttcaggaa atatcacaaa tgaaaaagca gataaaggtg
     1501 aaaattcaaa taaaaataat aaagacaaaa atgaagacaa atctaattct aaaagtaata
     1561 aagataagtc atcagaggac aaatcgtcag ataataaaaa tgaaattcaa aaaaataaaa
     1621 aacttttaat ttgtatagat ccagggcatc aaggaaaagg agatagcaat ttagaaccag
     1681 tagctccagg ttcttcctct aaaaaagcga gagtatcttc tggaacagag ggtattgcta
     1741 caaaaaaacc agaatatgtt ttaaacttag aagcatctct tgttttaaag agtatattag
     1801 aatctaaagg ttataatgta ataatgacta gagaaacaca tgatgtaaat ataagtaatt
     1861 cagaaagagc aatacttgct aatgataaaa aagcagatat ggttgtaaga atacatgcag
     1921 atagtttaaa taattcttca aaaactggag catctatatt gattcctgaa aaagatggaa
     1981 aatatacagc tcctatatat gaagagagta ataaatgtgc tgaatttatc aaacagaata
     2041 tggaacaatc aggtatacaa atcaatggaa tcgttcaaag aggtgatttg actggattca
     2101 actggtcaaa agtgccagct gtattagtag aaatgggatt tatgagtaac tataatgaag
     2161 accaaatgat gtcaaatcca gactaccaaa gaaaaatgat gcaatgtata gctgatggat
     2221 tggatgagta ttttaaataa gatatgaata gagaaaaagt taggctggta ttattttcct
     2281 aatttttatc tctatttttt attgtacata acacaaagat gaagtttatt ttaggttaaa
     2341 ttgtcgtaaa tataactaaa aatattggga atacttgatt taagtttaaa aagaatatat
     2401 tatagtatag aatgtgtaaa atttttttga gaggtgttga aatgctagta gtagaaaatg
     2461 tgagtcatgg atttggtgcg agaacaatat tagaaaatgt atcctttaga cttagaaaag
     2521 gtgaacatat tgcactagtt ggtgctaacg gagaaggaaa atcgagtttt ttaaatataa
     2581 taacaaagaa gcttatgcca gatgctggaa atataaaatg gtcttcaaga gctacagtag
     2641 gttacttaga ccaacatacg gtattaagta agggaaaaac tataagagaa gtgcttagag
     2701 aggcttttaa gcatatgttt gacttagagc aagaaatgat tgctatgtat gacaaaatgg
     2761 gagaagctag tgatgatgaa atgagtaatc ttcttgaaga aacagcagag atacaaacta
     2821 tacttgaaaa tagtggtttt tatatgatag atgcaaagat acaagaagtt gcaaatggtc
     2881 ttggtcttgg agaaattggt cttgataaag atgtaactga cttaagtgga gggcaaagaa
     2941 caaaagtact tcttactaaa cttctacttg aaaatccaac tattcttata ttggacgagc
     3001 ctacaaacta tttagacgaa gagcatataa catggcttac taagtacctt caagagtatg
     3061 agaatagttt tgtcttagtt tctcatgata ttgaatttat aaacaataca tgtaatgtaa
     3121 tatatcatat ggaaaatgga gagttaaata gatataaagg aaattatgat gaatttgtaa
     3181 gacttaatga tataaagaaa cgtcaagaag aacaagctta tgataaacaa gttgaagaaa
     3241 gaaaaagact agaggatttt gtagctagaa ataaagctag agtagcaact agaggaatgg
     3301 ctaacagtag acaaaagcaa cttgataaga tggaaatatt agaaagacca aaagaaaaaa
     3361 taaaacctac ttttgctttt aaagatgcta gagcagctag taaaataatt tttgaaacag
     3421 agaatttagt tttagggtat gatgaagcat tgacaaaacc acttaatttc catttagaaa
     3481 gaggaaaaaa gattgcctta aaagggatga atggtattgg aaaatctact ctattaaaaa
     3541 ctttacttgg aataataaaa ccatttgaag gaaatgttaa attaggtgac tatttggaag
     3601 ttggatattt tgaacaagag agttcaagag aaaatagtaa tacacctatg gatgaagttt
     3661 ggagtgaatt tccagggctt acaaactttg aagttagaca ggctcttgca aaatgtggac
     3721 ttacaaatga acatataact agtcaaatga gagtattaag tggtggagaa gctgctaagg
     3781 ttagactttg caaagtaatg ttaaaaaata taaatttctt agtattagat gagcctacaa
     3841 atcatttaga tgttgaggca aaagatgaat tgaagaaggc tatcaaagag tttaagggaa
     3901 cagttctttt agtatgccat gagccagaat tttattcaga aattgttgat gatgtatgga
     3961 atatagaaga ttttacaact aaaatcgtgt aatgatttaa cttattgctg gggggatttt
     4021 atgaaaaagt ttctaaatgt acttttttca gtttgtatta gtattgttat aatagtaggt
     4081 gttattaatt ttactgttgg atttaaacaa ctttattatt ttgacattga ttatttaaat
     4141 atatcagagt tatctggttt atctaaagat gatataaaat tgaactacga ttatctaatt
     4201 gattataatt taaataagaa tgtgtctgag ttcaagttac caacattaaa attttctcca
     4261 caagggaaga tacattttga agaagtcagg aatatatttc aaaatataaa taaattagct
     4321 aaattattat tggtagtttc tttagtagga attatattga gtgtaaaaaa taaaaatata
     4381 aaaatcttga aaactacgtc aataacactt ataattatgc cgttattatt aacagttcca
     4441 attttattaa attttgaaaa gtcatttatt atttttcata aattattatt tagaaatgat
     4501 tattgggtat ttaatccgaa tttagaccca gttataaata tattgccaga ggaatttttt
     4561 tttcactcag gaatgatgat tttaatatta attttgctag caagtatctt actatttgta
     4621 atgtataaat tatgtaaatc tttcagaata aaataagata acaaaaacga attatattaa
     4681 agtttataca ataaaaatag ctatgtaata atatttagac taagagtagt caataaatat
     4741 ttatgcatag ctatttctaa tagaaattac tttttactag tgattttaac taaatcttga
     4801 aatttcccca taatgtaatt gtaattatct tttttgtgag gtaacatgca actactgcaa
     4861 tctttgatac ctttatctgt atacttaaag tttccaccac aattttctcc taaagcatac
     4921 aaaggacaat agcaaaatag acagttaaac tcttctggtt tattagtttt atgacaaggg
     4981 aaaaattcgc aatctttgtg attaaaaaac ttataatttt cgcccatatt ttcctcctaa
     5041 ttaaataaaa tttaaatttt ttatactata taagattttg gtgataatat aattatacta
     5101 aataatttta tgaatagtcg ataatatagt attattaatg atagtattaa tggtataatt
     5161 agtcaacaga aaaataatgt aaaaataatg taagtgtatt ttgtaattag aatatgtttt
     5221 ataacaaaaa atgaggtgat taagtgaaag aaattcaaga aaaagcactt cttgtaggtc
     5281 tgaatttgac tacaatagtg aaaaaaaatg atgatataga cactagtgaa tctatggaag
     5341 aattaaaaga gctaacaaaa gctgctgggg cagaagttgt aggtagctta attcaaaata
     5401 aacactctgt agatgcagct tattatatag gaaaaggtaa agtagaagaa ataagagcct
     5461 atagtgactc tttagatgct actttagtaa tttttaatga tgaattatct ggtgctcaaa
     5521 tcagaaatat agagaatgtt gttggaagaa aagtaataga tagaactact ttaattttag
     5581 atatatttgc tcaaagggca cttagtaaag aaggtaaatt acaagttgaa ttagcacaat
     5641 taaaatatag attgccacga ctttatggaa tgggtggtga gatgagtaga acaggtgcag
     5701 ggattggtac tagaggtcct ggagaacaaa aactagaaat agataagaga catatactta
     5761 ataaagcagc agatataaga cgtgaactta aagaagtaaa aaagaataga gaaacacaga
     5821 gagttaagcg tttaaagtca aatataccga ttgtagcact tgttgggtat acaaatgcag
     5881 gaaaatctac cctgttgaac gaattaataa agacacacaa ggattatgaa caagaaaaag
     5941 aagtttttgt aaaagatatg ctttttgcaa ctttagatgt aaccttgaga aaggctcttt
     6001 tgccaaataa aaaagaattt ttagtagtag atactgttgg atttgtaagt aaacttcccc
     6061 atgatttagt agaagcgttt aaagcaacat tggaagaggt acagtatgca gatttaatct
     6121 tacatgtaat tgacgcaaca aatactagtt atgaattgca aaaaagtacg actgaaggtg
     6181 tgttaaaaga acttggtgta aatgataaaa aacatatact tgtatataat aaagtggaca
     6241 agctcgaatt ggatatatat cctaaaagtc aagaagatat tgtgtatatt tctgcaaaac
     6301 aaggcataaa tatggataag ttattaaata tgatagaaat tgctttaatg gaaaatactt
     6361 attcagtaag cttgatgtta ccttatgaga gaggtgatat atttagtaga ataaaagata
     6421 aatataatgt ggagaatttt gagtatgggg aaagtggtat aacgctagat gtagatttag
     6481 atgaagaaga ttttaatatc tatagagagt acatactaga aaaataggtt gaatatggat
     6541 ataaactggg aaaatataga taatcttgaa gattatttta taacctatct tctttataaa
     6601 gaatctaaaa cagtatctca aattagtaaa attagaaata tttcaagtac tgaagcaagt
     6661 gagcaactta tacaagcaaa gttaaaaata aaggaaatgc aaaaagatga ttttgaggca
     6721 tctaaggata ttttggataa atttttagaa ctagataaaa ttaagagact tgattttatg
     6781 gatagcttag atgacgaaaa aatggtatac tttaagagaa aggttttcaa aagaatcctt
     6841 gtagaaaaaa atgctgaaga tttaattgtt ttaatttggg cgacaggtga gctaaaagat
     6901 gataagtttt tgaaattgct tcatcagctc acaaaccaca gacactcaga tattagaaga
     6961 ataacttatt cagcaatcag aaagattgaa tcaccaagta gtagagaagt actgcaaaaa
     7021 ggtctctacg acaaaaatgc acagactagg cagtactgtg caaaagctct ttcaaaattg
     7081 ggagatgaaa acagtttaaa gattttacag cagttgaaag acaagaataa gaattttgaa
     7141 aaagaatatg tgcttagagc ttatgacgaa gccattaagt cattggaaaa tgcaaaaaat
     7201 ttgaaaaagt aattttttat taaagcaatt tgtttactta atgatgtatt tctaaagttt
     7261 aacaaggtaa taaataaatt aatgggaggg gtttcaaatg atagtaagac gttgtgcagg
     7321 tggagtagtg ttttatgcta ataaagtact tattgtaaaa aatgataggg gtgaatggac
     7381 acttccaaaa ggaaagatac taggaggggg acttccatat gagagtgctg ttcaaagagt
     7441 taaagtagaa acgggcatag atgctaaaat gatagatgtg gctggcgata ctatgtatga
     7501 atttttctca agaagtagac aacaagaagt ttgtaatgca ataatgtggt atgttatgga
     7561 agcttgtaac actgattgtg tacttgctcc tgaatttcaa gaaggtggat tttataaagt
     7621 aaaggatgct ttagaaatgt tatcacatca taaagagcaa gctttagtag aagtatctta
     7681 taaaaagttt aaggaattaa agaaattagt ttcagagggc gatgatgttc cagcaaatta
     7741 attgtgaatt tagaggattc tgaggaaggg atagcccctt ccttttttaa ttaaagcagt
     7801 tttaagcgta aattatatag tctctttaaa ttatgattta tcaatttaga gctttagtag
     7861 tttatattta aaatcagtaa gatatattta aaataaaaat taaaaagtat acaattttat
     7921 tatataattg tcttatgatg ttgattaaag aattattaaa agtccattta tttttaaata
     7981 gaataatttt ataaatatat atttgatgtg tgtattagaa aatgattaaa ataggaggaa
     8041 atacaatgag caattacagg acattacatg aatttggaac agatgaaata acgattgaaa
     8101 agtctgtttt tattggatat gctaagccta taaagagtga agaagaagca gtggagttta
     8161 taaatgaaat aaagaaaaag cataaagatg caactcacaa tgtgtgggct tatactgtag
     8221 gtaaaaatat gaatatacaa agatatagtg atgatggaga accacaagga acagctggga
     8281 tacctacact agaggttata aagaaagaag atttgagaga tgtagcggtt gttgttacta
     8341 ggtattttgg aggaacaaaa ttaggtgcag gtggtctagt tagagcttat acaaaaggtg
     8401 ctaaactggg acttgaagca ggtaagataa tttataaggt aatgtatcaa gaagttaagg
     8461 ttaagataga ctatactcaa cttggaaaag tgcaaaatga gttaatgaat ttggggtatt
     8521 ttataaaaga tactgtatat gaggataatg ttgagatagt agtgtattca agattagaag
     8581 atgtagagaa attatcagaa aaaatgattg atataacaag tggaacagga aaaataattt
     8641 taggagagga attttatttg tctgaacaaa atggagaaat tttaatataa tctaaaaggt
     8701 tattagaata gtcaatactt caaatagtca attggtattt ttaagtgaaa agccattaaa
     8761 ttgatttaat ctaaatcaat ttaatggctt tttatcagat tttgcacaga atcctttttt
     8821 tacttcaaat aaaccgacta aaagtaaaaa taccccaaat atttttttta atatatctga
     8881 agacagataa ttagctaata gtgaaccaaa tacagctcct aaaaccccaa acacagctat
     8941 aggtataacc aatttaaaaa ttacattttt attttttata tgtatagcaa gtgctataag
     9001 tgtcataggg atagatgaaa gtaaatttac gctttgtgct attttagtgt ctattccagc
     9061 aagtaaaacg agtgcaggta taaggattgt tcctccaccc attcccattc ctccaataat
     9121 tcctgcaaaa aatccaatta gagcaaataa cattaaaaca ccatccttaa tgctgcaatt
     9181 atcatgatta ttccaaaaga aattctaaga aattttccag taaccttatt tagcaattta
     9241 gctcctataa taccaccaag aatacttcca actgcaactt taattgttaa gttaacgtcg
     9301 taagtacctt tagatacata taaaacagaa cttgctgtcg ttaagaatat tattatagaa
     9361 agagcagttg catgagattt atgttcttct accttaacga tgtcatttaa tattggaact
     9421 aaaagagtac ctcctccaga accaaaaact ccatttataa atcctgtaaa aactccaatt
     9481 atcgtatttt ttaagttaaa atcaaagtat tttttcttta aatcatcagt ttttttcata
     9541 aaaatcacct tctgaaatag ttttaccaaa ataggcaaat tatatttata taaaataaga
     9601 ttaaatataa tctttcatgt ttgcttaatt ttgcataaaa aaaaacatat taaaacaaca
     9661 tattaatata tggtataata tattaaaaca aagtagcaaa tttgagagga gataacaatg
     9721 tcaaatataa aaatacaaat agacaaaact gtagaatggc ttataaataa ggttaatgag
     9781 gcaaacgcaa aaggattaat tgttggtgta tctggaggaa ttgattcagc agtagttgct
     9841 aatttaatta aaaaggcatt tccagaaaac tctatgggag ttataatgag cataaaaagt
     9901 aatcctcaag atagagaaga tgcacttaag gttatagagg gatgtgatat agaatacctt
     9961 gatttagacc ttatagaacc acaaagtgct atattagaca tggtagtagg aaatttaaaa
    10021 gataaacatt tatatagaga agaatattta aagatgacag atgcaaactt aagagcaaga
    10081 gtaagaatga gtacaattta tacaattgct aacaatctag gatatctggt tgttggtaca
    10141 gacaatgctg cagaaataca cacaggatat tttactaaat ttggtgatgg tggagttgat
    10201 attttaccta ttgcaaattt aactaaagga gaggtgtatg aatgggcaaa agagcttggt
    10261 gttcatgaag atttaataaa caaagctcct tcagcaggtc tttgggaagg tcaaacagat
    10321 gaagatgaaa tgggaactac ttacaatatg atagatgctg tgttagaagg tagattggat
    10381 gaagttccta aaagagacca agagataatt gaaagacttc atagattaag tgaacacaag
    10441 agaaaaacgc ctgcacaacc tcctaagttc taatttacta tttataagat ttgtgataaa
    10501 atttacaagt gatgaaaatt agaataatta attgtacgta ttaaggagtg gaaatatggg
    10561 acgtataggt aatataatta atagaaaagg taaacaagat gctcaaagag caaagatatt
    10621 tactaaacat gctagagcaa tagcagttgc agctaaagaa ggtggagcag acccagagta
    10681 caatgcagcg ttaaaaacag ctatagaaaa agctaaggca gataatatgc caaatgataa
    10741 tatagataga gctatcgcta aaggtgctgg agcaggtgct ggagaagact atgaaactat
    10801 agtttatgaa ggatatggac ctggtggagt tgctgttata gtagaaactt taactgacaa
    10861 caaaaacaga actgctggta acgtaagata ttattttgac aaaaacggag gaaacttagg
    10921 aactagtgga tgtgtgtctt ttatgtttga taaaaaagga caaatattag ttggtttagg
    10981 agatggagtt tcagaagaag aattgatgga tgttgcatta gaagcaggag ctgaagattt
    11041 cataacagaa gaagatggtt atgaaataat aactacacca gaagattttt ctagtgttcg
    11101 tgatgagtta aaagctaaag gatatgagtt tatatctgct gatgtgaaga tgatacctca
    11161 aactacgact gtattgactg aagaatctca tttaaaaatg atgaataaat tagttgatat
    11221 gcttgaagaa gatgatgatg ttcaagatat atatcataac tgggaagtag aataaatgtt
    11281 ataagcatat tgaaatttat aagtattgaa tcaattttaa catgtgataa attttatgga
    11341 ataagaaatt actaaaatag acatacgcac ctctaaccta tatttgcata ataatatgta
    11401 agcatagata aggggtgttt ttatatttat aggttaagaa atttaaaaaa atggagttat
    11461 ttgatttaga ataatatcaa aggagattaa cttaacattg caaatctaat ctaaaattgt
    11521 cagaaacatt taagatagga aaagttactt agacactaag tctttgtggc ttttcctatt
    11581 tttgttaaga aatttctact attagagcag atatatactg aactaaagta ttaatggttt
    11641 aaatcaatag aaaataaatt atctactatg ttgaataaag attccttata tcagagtgaa
    11701 aataaactta tagagatgtt atttaaatta ttttaatgaa aatatcaaaa atgagactgt
    11761 attctcaaaa atgagactgt gttttttaag tattttctcg aaaatgagac aaaaatattg
    11821 ttttatgggt tctattgaag aaaaattctt atgtgaatta caatttaatc gattttaaag
    11881 ttgaaaatta ttaaaaattt agtgaaattt atgtatattt cgtttatttt attaaaaata
    11941 acaatttttt actaaaaata aagaacttca atggtgattg gcatagtact tgctatttta
    12001 tattaacaag aaatgtagaa aaaataacaa tgtataatgg ggggaatatg atggaatctg
    12061 taatggaaaa tttgaacaat cttaaggtgg aggttaagga caaagtctgt gttattacta
    12121 taaatagacc caaagcttta aatgctctta atagtgacac ccttagggaa ctatcacaag
    12181 ttattgatgt agtatcagaa aatgaggcaa tattaggagt tattattact ggagaaggta
    12241 aagtttttgt agctggtgct gatatacgcc agatgcaaaa ctataaaagt gaggaaggca
    12301 gaaaatatgc aggttatgct cagggaattt ttgataaaat tgaagctctt gagaaaacag
    12361 taatagcagc agttaatgga tatgcattag gtggtggatg tgaacttgct atgagttgtg
    12421 atattagaat tgcatcagaa aaggctatat ttggacaacc tgaagttaat ttaggagtta
    12481 ttccatgttt tggtggaact caaagattat caagacttgt tggaactgga atagccaaag
    12541 agttaatttt tacggggaga caagtaaatg cagaagaagc taaatctatt gggcttatta
    12601 ataaagtggt tccaagtgat ttacttttag aagagtctat gaagatgatg aaccaaatag
    12661 ttgaaaaagc gccaatagca ataagatatg caaaagttgt tattaacaaa gggatagata
    12721 tggatttaaa gaatgctctt gaactagaga aagatattgc aggtttaact tttgctacaa
    12781 gagataagca agaaggaatg aatgcattta tagaaaagag aaagccagtt tttgaaaata
    12841 agtagctatt ataactaaat tggaacaatg tagaatatat tcaaaatcta aatttcgtga
    12901 aaaaatacat atagtttaat tttaaaagga gttaatttat gaatacggga gtaattataa
    12961 gtttagtagg gattatcata gctctatctc ttctagttgt aatggttatg aagggaataa
    13021 atatttttat tatagcaatt gtatgttcat cattagttgc tataacagga agtctaaatg
    13081 tgtatgatgc acttaaggtg gattatatga cagggtttgt tggatttctt caggcaaatt
    13141 tctttatttt cctaacaggt acattaatgg gaaaaataat ggagattact ggtggagcaa
    13201 aatctattgc taagatgatt gtaagatgga ttgggaagga taaagcacta ctttctattc
    13261 caatagcttg tggaatatta gcctatggtg gagtaagtgt tttcgttgta agttttgcag
    13321 tgtttccgat agctttagaa atttttaaag aagctgattt gccacgaaga tttataccag
    13381 cagctttgac ttttggatgc tcaacttttg ctatggttgc accaggggca ccccaaattc
    13441 agaatattgt tccagcatca acattaggta cagatattat ggctggagca gtaaatggtt
    13501 ttatatcatg tgcagtgatg tttatagttg gaagcaccat tttgtataaa atggtttcaa
    13561 aagaaaaagc caatggtggt cattttatag cacatgaatc agatatattt gatgaagcag
    13621 aagcaactaa ttctaagaga gaaaatggtc ctaatggtgt aattgcctta attccattaa
    13681 tagtgtcaat tttaataatt aatgtaaaag ttaatggaaa agcgatagta ccaattgagg
    13741 tgggagtatt tattggagct ttattagtgt atttattgtt aaataaatat caagataata
    13801 ctaaaatagt tggacatgtt ggtgatgcat gtaaaactac agtagtagca atatgtaata
    13861 cttgtgctgt agttgcattt ggttcagtag taaaatcagc agttggtttt gatttcatag
    13921 ttaatgccat gactaatatt cctggtcctc ctattgcagg ggctgcagtt ggaactacag
    13981 ttattgcagg tatttgtggg tcagcttcag gtggactagg aattgcagct ccacttcttg
    14041 gaccagtatt tttagctaag ggtgtttctg ctgcagcatt aactaggact atggcaattt
    14101 catcagcagc attagattca cttccacaca atggatatat tgttactgta acaaatggtc
    14161 tgtgtaaaga aacgcataag gacgcctata tgccaatatt ctgggtaact gttttaactc
    14221 cgtttattgg aaccatagta gcagttctac tgtttacaat gttcccagga ttaccataag
    14281 agagaagata aaaggtataa attatatttt gatattataa aatcaatttt caaaatatat
    14341 acaataaatt tcatgtttta atactgtaaa tatgtattta aaataacaaa ttgaccatat
    14401 aaggaggaat tgaaagtgtt aaataaacca ttagagggag ttaaggttat tgacctaacg
    14461 tattttgtag caggtccagg gacttctaaa atattagctg actggggcgc tgacgttatt
    14521 aaggtagagc caagctttgg agacccagga agaaagacag gggcaacaat gacaatgcca
    14581 atagatgatt ataataatcc tttttacagc acatataatt caaataaaag aggtttgtct
    14641 ataaatctta agagtgaaac tggaattgaa attatggata aacttttaag tgaagcaaac
    14701 gtatttgttt caagttatag aacaggagcg ttaaaaagac taggtcttga ttatgaatca
    14761 ttaagtaaaa agcatccaca ccttatctgg ggtcagataa atggttttgg cgattttgga
    14821 cctgctaagg acaatgcagg atttgataca gttgcctttt gggcaagaag tggagctatg
    14881 cttgatatag cagaaaaaga tacaagccca ataaatccgc ctatagcttt tggagatgca
    14941 accacttctt gtagtttatc tggaggaata tgtgcagctt tgtaccagca agctaagact
    15001 ggaaaaggac aaaaagtaat ggcatcttta tttgggcaag caatatggaa tgcatcatct
    15061 ttattagcat ctacacagtt tagtgatgag tatccaaaaa ctagaaaaaa tgctatttca
    15121 ccagtaataa attcttatcg ttgtaaggat ggaaagtgga tattcttaag tattttagag
    15181 catgaaagat attttgaggc actttgtaga ttatttggaa gagaagactt aataaaagaa
    15241 gagaaatttg catcttcagt agaaagtaag aaaaatgcta cagaacttat taatattttg
    15301 gatggtgaat ttgcaaagtt tactcaaaat gagatggttg aaaaacttac tgctgctgat
    15361 atagctcatg aaaaaattca acatgttaat gatgtagtga ctgatgaaca ggctattgct
    15421 aataactata tatatgaaca tacaagtaaa aatggaaata agacaatgct tgcatctact
    15481 ccagttaaat ttggaaatat tgaagttaac atgacttgtg atgctccact tataggagaa
    15541 cattcagatg agatattgag agaacttgga tacagtgatt ctgatataga ggctctaatt
    15601 gaatctggaa ttgtaacaat gaataaagaa gcatgtgaaa tatgttaata aacatgtgaa
    15661 gtatgttaat aaataggagg gaattttatg gattttagat taacagaagc acaattaatg
    15721 cttcaaagag tagcaaaaga atttgcagag aatgaaatag caccaatagc tgctgaaaca
    15781 gataaaacag gtatttttcc aagagagctt tttagcaaaa tggctaaaat aggatttaat
    15841 ggaattggaa cacctgtaga gtatagtggt tctggtgggg cagatataga aaaggtaatt
    15901 gttgttactg agattgccaa aaaatgtgca gcatctgcag caatattatc aatacacaca
    15961 atatatgctc aagctatttt gaaatttggt actgaggaac aaaagaaaaa ataccttcca
    16021 atgatggctg aaggtgggtg tgtaggtgct tttgccttaa ctgaacctaa tgctggttca
    16081 gatgctgcaa gagctgcaac aactgcaatt attgatgaag aaactgatga atatgtttta
    16141 aatggaacta agtgttttat ttctggtgga ggtcaagcag aatcattaat aatatttgca
    16201 ctgacagacc catcaaaggg aataaaggga atgtctgcta tcattgtcga caaaggtact
    16261 ccaggttttt caataggaaa aattgaagaa aaaatgggta ttcatggttc agaaactgca
    16321 gagttaatat ttgataactg tagagttcca aagtctaact tattaggaaa agaaggcaaa
    16381 ggatttaata tagctatgac ttgtttagat ggagcaagaa ttggagttgg agctcaagct
    16441 gtaggaattg ctgaaggggc tttagaagaa agtatcaaat attctaaaga aagagtccag
    16501 tttggcaagc caatttcagc tttgcaaggt attcaatggt atatagcaga tatggcgaca
    16561 atggtggaat cagcaaaatt attagtatat tatgctgcag atttgaaagc tagaggagaa
    16621 aaacacacta aagaagctgc tatggcaaaa tacaatgcgt cacgtactgc aagagaggtt
    16681 acaaatttag cccttcaaat ccatggtgga tatggatata tgaaggatta tccattagag
    16741 agaatgtaca gagatgccaa aataacagaa atttacgaag gtacttcaga aatacacaaa
    16801 gttgttattt caagagcagt actaggatag gaggaaaaga atgaatatag ttgtttgctt
    16861 aaagcaagtt ccagatacta atgaagttaa aataaataaa gagacaggta ctctaataag
    16921 agatggtgta ccaagtataa tcaatccaga tgatagaaat gctttagaag aagctctaaa
    16981 aatgaaagat gaactaggag cagtgattaa ggttataagc atgggacctc ctcaagctaa
    17041 aagtgcttta aaagaagctt tggcaatggg agctgatgaa gcttatttaa tctcagatag
    17101 agcttttggt ggttcagata cttgggctac atctacaatt attgctgctg ctatagagaa
    17161 ggttggaaag tatgatgtaa tattctgcgg aagacaagct atagatggag atacagctca
    17221 ggttggacca gaggttgcag aatttttagg gataccacaa gttacttatg cgaaagaagt
    17281 aaaggttcaa gatgacaaat tactagtaac tcgttataca gagacagggg attatttaat
    17341 agaagcaaag atgccagttt tattaactgc tataaaagag ttaaacaatc caagatatcc
    17401 aagtgtcaaa ggaatcttag aagcttataa taatggagat gcaaagatta ctgtattaac
    17461 acttgcagat ttagatgttg atacaactca aattggatta aagggttcac ctacaaacgt
    17521 ttataagtct tttgtaccag ttaaagacaa gcataatgaa ataatagaag gtataaataa
    17581 aaaagaaaaa gctgagaaac taatagaaat actattcgat ttgaaactag tataggagtg
    17641 ttaagatgat gagagcaaaa gttaatcaag gtataaattt aaatgattat aatggtgttt
    17701 gggtaatagg agagcaaagg gaaggtaaaa ttaatcctgt tacgatagaa cttattggtg
    17761 aaggaagaaa gcttgctgac caattaggaa aagagcttgc agttgtgata gctggatatg
    17821 aagtagagaa agaagtgaag gaattacttc actatagtgt tgataaagta tattacatca
    17881 atgaccctct tcttaaagat ttcactactg atggatatgc aatttcaatt gcaaatttaa
    17941 tcgaaagaaa aaagccagag gttgtattag ttggtgctac ttctatagga agagatatag
    18001 cgcctagaat tgcaggtaag gttggaacag gtctaactgc tgactgtaca aaacttgaaa
    18061 tagattcaac agataataag ttattacaaa caagaccagc atttggtgga aatttaatgg
    18121 ccactattgt ttgtccaaaa aatagaccac agatgtcaac agttcgtcca ggagttatgg
    18181 caaaagctgt aagaaacgaa tcagagactg gaattttaga agttgttact ccagaactta
    18241 ctgaaaaaat gattagaact agattagtag agatacttcc acaagaaaag aaatctgtaa
    18301 atctaactga tgctagaata atagtatcag ggggaagagg attaaaaaga gcagaaggat
    18361 ttgagcttat aaaagaattg gctgataaat tgggggcaga gattggtgct tcaagagcag
    18421 cagtagattc agggtggata gaacattctc atcaagtggg acaaacagga actactgtta
    18481 gaccagaact atacattgca tgtggaatat ctggagcaat acaacattta gcaggtatga
    18541 gtgattctaa atatatcgtt gctataaata aagatgcaaa agcccctatt tttagtatat
    18601 gtgactatgg aatagtagga gatttatatg aaattatacc tgaaatgata gaatcattaa
    18661 ataggtaact tctagaaaac attgaatatg tggaatgata agtcttataa tattgtattg
    18721 taagacttat tatttttgct gaaggttata gtggggagat gaaacaatga aagagcagtg
    18781 gtataaggat atatttgcaa gagtattatc tatgactgat gatggtttta ttgttgtaaa
    18841 tacaagtgga gtaataatag atattaacga taaatattgt gactttttag gaaaagaaag
    18901 aaaagatata ataggacaaa atatacaaag tattatacca aatacaaaaa tgctagacgt
    18961 tatgaaagat aagtactgtg aagaaggagc tatacatcat tattcaggtg gaaatacaaa
    19021 agaaaaaagt gtaatagtaa gtaggtctta tgtggaaaat gacaatggag aagtagttgc
    19081 aggagtagct caagttaaat ttagattgca atcatttgat gtggctaaaa agctaatgtc
    19141 agaatatatg gagctccaat attataaaga gcagtttaag gataactgtg gttttgacaa
    19201 gctcataggt gaaaatagag actttataga acttaagaaa acaggtgtga aagcttctaa
    19261 aacaaacttt ccagtacttt taacgggtga aacaggcaca ggtaaggaag tctttgctag
    19321 agcaattcac aataatagca gtaggtcaga caaacctatg gttagtataa actgcgctgc
    19381 aataccagaa gaattattag agtcagagct atttggttat gacgaaggag cttttacagg
    19441 agcaaaaaag ggtggcaaaa aaggtaagtt tttagttgcg aataatggta caatcttttt
    19501 agatgagata ggtgacatgc ctttgaccat gcaagcaaaa cttttaagag ttcttcagga
    19561 aagtgagata gaaccagtgg gaggtcttaa aactataaag atagatgtaa gagtaatctc
    19621 tgctacaaga aagaatttaa gtaaaatggt tgaagaggga ttatttagag aagacctata
    19681 ctatagatta aatgtaataa acattcatat gatggaattg aaggataggc aagacgatat
    19741 tttattgcta gcaaattata tattaaacaa attaaatgtt gaatacaagg aattgaaggt
    19801 tttaagtgat aaggttaaaa attgttttat taactatact tggccaggaa atataagaga
    19861 actacaaaat gtaataaaaa gtgcctatgc tgtaagtgat gatatggtca ttatgatgtg
    19921 tgaccttcca tctaagatgg ataatatcag cagagtagct caatgtaatg tggatagcaa
    19981 ctgttcaatt catgaaatgg ttgagaatta tgaaaagagc ttaattattg atgtgttaag
    20041 gaagtataat tggaaatgtt ctaaggcagc agaggttatg ggaatacaca agagtctttt
    20101 atataagaaa ataaaaaagt atgaaattga attaaataat taagataact tgaattaagg
    20161 caatgctaaa aaaagcgccc ttattcaaag cgaactgtat aaactgtttt atgcagttaa
    20221 aacgtgtaaa agtagatggg aagtactagt gtcaatcatt agtacttttt tattgtattt
    20281 ataattacta tttatatttc agtatctttt gatatttaag attattatgt ttaatttagg
    20341 acataacaaa accctcttag ttatggacag ttttttatat aactgtctac taagagggga
    20401 tagtttagtt caattttaga gtaaatttta tctagcattt ataaaaaata ttttgaatag
    20461 cttttttgtt atttaatcat aattttctac ttctttatca agttctttgc cacaagaatc
    20521 acacttatat atagcattgt atccgtaatc atattcatca actattttct tttgtctaaa
    20581 tacaccacca cactcacata ggaaaccaat ttttataagt gtaacgataa attgtgattt
    20641 tataaatttt atatcattac atttatcgtt agtgtaaaat gtataaatat aagagttgtc
    20701 gcactcatct cttaattcaa atctttcaat atatgtgtta ttttttaatt cctcaaattc
    20761 ttctttagtt aaatatgatt tttgttgcaa ttcatgtaaa cgcttaattt ggtccattat
    20821 ttatatcctc ccaataattt tagtatttta agcttagata aggaatctaa gttttattaa
    20881 atttatacca aggaattatt taaatcatat ttagttttat taattacaac atatataaac
    20941 tacacgaaaa taaaactaat tgtaaaaata atatcctgtg aaaacctaaa ctactaaccc
    21001 cttactaacc cgaaaactaa aaaaagaaaa tatatccaaa tataagtgat atataaataa
    21061 agatgtttta tataaattta actttaaata ttctgacaat aatata
//
LOCUS       DAEPXF010000061        21024 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain HB27
            SAMN13376389-rid12479423.denovo.065, whole genome shotgun sequence.
ACCESSION   DAEPXF010000061 DAEPXF010000000
VERSION     DAEPXF010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13376389
            Sequence Read Archive: SRR10518432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 21024)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21024)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001069624.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001069624.1
            Genome Coverage       :: 315x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/15/2021 01:54:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,165
            CDSs (total)                      :: 4,076
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 89
            rRNAs                             :: 10, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 10 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 19 of 69
            Pseudo Genes (incomplete)         :: 44 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 10 of 69
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21024
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13376389-rid12479423.denovo.065"
                     /strain="HB27"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="China: Hebei"
                     /collection_date="2017"
     gene            complement(39..899)
                     /locus_tag="KRL67_001885"
     CDS             complement(39..899)
                     /locus_tag="KRL67_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-ADP-ribose hydrolase"
                     /protein_id="HBH1522765.1"
                     /translation="MAMKWRDYADDINLFEDFDKTIKPLTDEQRRKNINTLIAYFSKE
                     VPSKIYNLSNDEIKPRDILRGLLNVYPPKEIAPEILNMLHNLLLIECEERELVDVNDI
                     EEVEEGIAIWRGNITNLRADAIVNAANNKLLGCLQPLHLCVDNEIHSCAGPRLREDCD
                     KIIKKQGHLEYTGDAKITRGYCLPAKFVVHTVGPIVSGGQPSKEQEKQLLHCYKSCLN
                     TIKEIDEIKNIVFCGISTGVFGYPKKEAANLAVSRVRLWLKENPEKNLKVVFNVFTEE
                     EEEKYRRIFK"
     gene            complement(1455..2783)
                     /locus_tag="KRL67_001886"
     CDS             complement(1455..2783)
                     /locus_tag="KRL67_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010963854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-alpha-glucosidase"
                     /protein_id="HBH1522766.1"
                     /translation="MEKFSVVIAGGGSTYTPEIILMLLDNLDRLPLRAIKLYDNDEER
                     QNKVAKACEILIKEKDPNIEYLATTCPKEAYTDVDFCLAHIRVGKLEMRELDEKIPLR
                     HGVVGQETCGPGGIAYGMRSIEGVLENIDYMEKYSPNCWMLNYSNPASIIAEAVRRLR
                     PNSRVINICDMPIGMEHNIARIAGLKSRKHMDIRYFGLNHFGWYTSIKDKKGNELLPK
                     LVEHIKEYGFINGEEGMKNDKKDSWFETNLFTKEIVKTDLTTIPSSYLKYYLFPDYVV
                     NHSDVNYTRANEVIDGREKEVFGSCALIEKQGHSKGSGLKIGIHAEFIVDLATALAFN
                     TQERMLLIVQNNGAIENIQDDAMVEIPCIVGKDGYEPLSIGKIPTFQKGLIEQQLAVE
                     KLVVDAWIEKSYNKLWQALMLSKTVPSATVAKELLDDLIEANRNYWPELN"
     gene            complement(2931..3260)
                     /locus_tag="KRL67_001887"
     CDS             complement(2931..3260)
                     /locus_tag="KRL67_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HBH1522767.1"
                     /translation="MVPEMEEKVFSIISEAGDAKSDVMMSLKEIKKGDYNKAKHLLNS
                     ASEKIQTASKHHLELLSNAMNSEDSGTDFLVVHSEDHYSNALFAHSLVSELVDIFEMM
                     DPRINKN"
     gene            complement(3284..4573)
                     /locus_tag="KRL67_001888"
     CDS             complement(3284..4573)
                     /locus_tag="KRL67_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBH1522768.1"
                     /translation="MEALQNNLRKFLLPIAQKIEKQRHLQAIKEGMISITPIIIVGSL
                     SMLFMALNNMLPEGSAKTLLSENMDTLLIPNKFTMSLLSIYSAFFIAQALAKKYNLNH
                     VEIGMTAVVAQLVVCGQVVDGVLDTSYLDAQGLFVSILVALLVVDITKFMNDKNLVIR
                     FPKEVPSVVNKSFRNLTPMIVCIMLFTAIAAITKNVSGQPLPAIIMNFLAPAISSVDN
                     VFAVTIILFITQLLWFFGLHGAAITSSIWMPIAATYMAENATLIAAGGDPKYVFTIGF
                     YYGFLQVTGSGITLGLVYLMSRSKCKSFNSMGKVVILPSLFGINEPVIFGTPIVMNPY
                     MFVPFVFGPVLVGALNFMALKVGLVGLPIAEPPGFLPPGVAAFLMTLDWKAIVLVFAS
                     IILMTLIYYPFFKIMEKEELNKNAELATTLDDDSFDF"
     gene            complement(4589..4915)
                     /locus_tag="KRL67_001889"
     CDS             complement(4589..4915)
                     /locus_tag="KRL67_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBH1522769.1"
                     /translation="MNILLVCANGASTGVLVEKMKSFCSEHEKLKTKTINIEATSFEN
                     LKSYIEANDTDVVLVAPQIRFKEDDVVEACKNYKIAVGLIDTKHYGRMDAPSVMKSAI
                     ELYKNR"
     gene            complement(5050..5679)
                     /locus_tag="KRL67_001890"
     CDS             complement(5050..5679)
                     /locus_tag="KRL67_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH1522770.1"
                     /translation="MQDKILRDKKANDPWARMTTRNGFSADEIISSFQKAIRRNMVEE
                     ACEFAYELYISSPELEDKLWRRILTISVEDIGMGDPSAVIIINNLNQVRKEYSYADGD
                     RPLFFIHAIRYLCACEKDRSSDLLKNIIIKGFAMGKVPEVMDVALDKHTYRGLEMGRD
                     SFHFLNEASIVIPEKEVDNDYKERYLKILEKYKQEEVIDTAFKFNHWQY"
     gene            complement(5894..8551)
                     /locus_tag="KRL67_001891"
                     /pseudo
     CDS             complement(5894..8551)
                     /locus_tag="KRL67_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418584.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
     gene            complement(8910..9854)
                     /locus_tag="KRL67_001892"
     CDS             complement(8910..9854)
                     /locus_tag="KRL67_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009524518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBH1522771.1"
                     /translation="MNREKILEIKNLKQYFHLDKSTTVKAVDDISFDIYKGEIFGLVG
                     ESGSGKSTTGKTIIRLHESTGGEVIYKGNCISDKKTYKFIKKDVNKSMQIIFQDSTSS
                     LNPRMTIADIISEPLKIQGICKNKTDRMDKVYEMLNLVGLDRSYANKYPSDFSGGQRQ
                     RIGIARALSVDPEFIIADEPIASLDVSIQAQIVNLFKKLQQEKNLTCLFIAHDLSMVR
                     HISDRIGVMYNGKLVELADSNELYNNPIHPYTKSLLSAIPVPDPRYAKARNRIEYNSN
                     VYDCSNEKSLSWIEISDGHFVYSSKSDINKYQQNLKVV"
     gene            complement(9847..10872)
                     /locus_tag="KRL67_001893"
     CDS             complement(9847..10872)
                     /locus_tag="KRL67_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBH1522772.1"
                     /translation="MEHLLEVNNLSVSFKVEEGEVQAVRNVSFNLKKGETLAIVGESG
                     CGKSVLCKSLMRILPYNGYIKNGEVLLKSGDLVKKSEKEMEDIRGKNISMIFQDPMTS
                     LNPTISIGKQIAEAVIIHQGISKSEAKKRAIELIELVGIDNPEKRFKQFPHHFSGGMR
                     QRIVIAIALACNPDVLIADEPTTALDVTIQAQIIDLIKDLQHKIGLSIIFITHDLGVV
                     ATIADRIAVMYAGKIVEIGTVEDIFYDPRHPYTWGLLGSLPTLDSQDDYLYNIPGMPP
                     NLLNPPKGDAFAIRNKNALKIDYEKEPPMFKINDTHSAATWLLHPDAPEVDVPVRVNC
                     GRVISNE"
     gene            complement(10947..12515)
                     /locus_tag="KRL67_001894"
     CDS             complement(10947..12515)
                     /locus_tag="KRL67_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBH1522773.1"
                     /translation="MKLKKLKVLSLVMILSLMAGCSSGGDKDKKADTPKDGKVLVYGS
                     NDYTSINPALYEHGEINSLIFNGLTAHDENNKVVPCLAKDWKFDEATNTYTFNLRDDV
                     KWHDGEKFTANDVKFTIETIMNPDNASEIASNYEDITKIDVVNDNTIKITLKAPNTAM
                     LDYLTVGILPKHALEGKDIATDEFNQKPIGTGPFKLEKWDKGQSITLVKNSDYFVKEP
                     GLDKVVFKIVPDDKAKAMQLKSGELDLAQVTPKDMSNFEKDEKNFKVNIMKTADYRGI
                     LYNFNSKFFKDKKAKGLPNALSYAIDRKAIVDSVLLGHGVPAYSPLQMGPYNNPDIEK
                     FEYNPEKAKQEIEKLGWKLGSDGIYEKEGTKLAFEITAGESDQVRVDMAKICAQQLKE
                     IGVDAKAVVVTETDWANQDAHLIGWGSPFDPDDHTYKVFGTDKGANYSAYSNPTIDKI
                     LQKARETEDKDEKLKLYKQFQVEMTKDMPYTFIAYIDAIYVGKPNIKGLTPDTVLGHH
                     GVGIFWNIADWTIE"
     gene            complement(12628..13539)
                     /locus_tag="KRL67_001895"
     CDS             complement(12628..13539)
                     /locus_tag="KRL67_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBH1522774.1"
                     /translation="MKPQYSDFEIVGENYILVAEDEHIETKKTLSKKIKELPYISIII
                     LSIIVIGSVFSSFIMTHEPTYMDLASSNLAPNKTFLFGTDSMGRDIYSMIWYGGKISL
                     FIGLFSTVISTVIGIVYGSISGSASELVDDAMMRFTEIILSIPSILIIIFVQAILGNS
                     NPVSMSIVIGITSWMNISKIVRTEVRQIRNSEYILAAKSMGGGFFYVLKQHLLPNFVA
                     SIMFMVVTNIGAAIGTESTLSFLGIGLPIEIVSWGSMLSLSEEALLSNRWWIILIPGI
                     FLVTTLVCITNIGNYIRKNNNKKSSNL"
     gene            complement(13523..14509)
                     /locus_tag="KRL67_001896"
     CDS             complement(13523..14509)
                     /locus_tag="KRL67_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBH1522775.1"
                     /translation="MLKDILKKLLQFILVMFLLSFIVFYMARLAPGDPLISYYGDGVE
                     RMSTQEKENAMKKLGLDEPIYSQYIKWISNASKGEFGISFKYKQNVTSVINDVYINTI
                     ILGGSGYILTFVLALLLGIFCTLHEDKLIDRIICKLGTITNCIPSFWVALVLILIFSI
                     NLSILPSSGAYSMGEESSILSRISHLILPLTVLILSHLWYYTYMIRNKLLEEIREDYV
                     LLCKAKGLNNRTIVFKHCLRNIMPSYISIMAISIPHILGGTYVVEKVFSYPGLGTLCF
                     ESAKYHDYNMLLVLCLITGALVVFGNMLAQIINNKIDPRMKYDRGDTNEATI"
     gene            14869..15270
                     /locus_tag="KRL67_001897"
     CDS             14869..15270
                     /locus_tag="KRL67_001897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HBH1522776.1"
                     /translation="MLLKELYSKTMYTVEENFKENGLTHQQIIVIKLIAHNQELTISQ
                     LCDEMSLAKGTVSGIISRLEQIGYIEKFKKSNDKRNTYVKFTTTGFEFATNFKIKMQE
                     SFDDIFKNCDENELSDLVKNLRNILAKVKER"
     gene            15273..15671
                     /locus_tag="KRL67_001898"
     CDS             15273..15671
                     /locus_tag="KRL67_001898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03987 family protein"
                     /protein_id="HBH1522777.1"
                     /translation="MNYKLILAIVFITSALIFYTIGVFGERKAKILKKKHVIIFWLGF
                     IFDTLGTFTMSNIANSHTFEVKSALSQNLHSITGLLAIVLMLFHASWATFVLYKDDEE
                     KKKFFHKFSIVVWTIWLVPYFIGMFIGMAG"
     gene            complement(15999..16223)
                     /locus_tag="KRL67_001899"
     CDS             complement(15999..16223)
                     /locus_tag="KRL67_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifU family protein"
                     /protein_id="HBH1522778.1"
                     /translation="MREKVEKVLEEKIKPVLQRDGGDVELIDVNENGVVLVRLQGACS
                     GCPGATMTIKAIIENVLVSEVPGVTQVLGV"
     gene            complement(16258..17625)
                     /locus_tag="KRL67_001900"
     CDS             complement(16258..17625)
                     /locus_tag="KRL67_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HBH1522779.1"
                     /translation="MKEYIINNVEEIREELIDLSKKIWENPELAFEEKYASSIQKEYL
                     KSKGFKIEEVENLPTGFIASFGEGKPVIGILGEYDALPELSQCVSAERKPLVEGKAGH
                     GCGHNLLGVAGVGAVVSIKKLIEENKFNGTIKYFGCPAEEEGGGKTVMCINGCFDDVD
                     CAFTWHPFDINAPWRGGSLANLSVKFKFKGITAHAAQAPHNGRSALDAVEIMNVGANY
                     LREHVIDSIRMHYVITNGGGRPNVVPGFAESWYFIRGKKAKDAEHVLDRLIKVAQGAA
                     MMTETEMEYKVTDGIYDYIPNQCLTDLVYDNMVFVGCPKNTPEEEEFAKKLCDTLTRE
                     ERLGVATSLQNDKSIVESYIHKEIVDGDKDKGLAGSTDVGDVSYVIPVAQFAMAAWPV
                     GIASHTWQSCSSAGSNIGFSAMINSAKVLACSAYDVFMDTKIIDEAKIEFDKSLDGQK
                     FKPLV"
     gene            complement(17709..19244)
                     /locus_tag="KRL67_001901"
     CDS             complement(17709..19244)
                     /locus_tag="KRL67_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH1522780.1"
                     /translation="MLNNLSNININENNLIKNQYSISLLKECLNCKVIAEREVYNIQQ
                     EISLILMDLIKKYTNGQSTSVKTEVAEKLLISIWYAIDAYINKLGDIEKRVVELTAKN
                     VKKVYLGGIEILEEELVRLKDFYELMVSRKLNTNSIAYNDTLMELSSFFKSYIIKFEA
                     QDVPVSIDYPLALDDMDVKGIYYVKNYIESIDIENRFCNLFKKKDREKLFYDYGVTYK
                     IDCDVILVNLFELIINNCIFSTILGNEVTNLEISKYEYEFLEKQFKNLYNNITEDKVI
                     GSEESNIIELIEVDDSFNTEVDSNLDYNDFEEINREIRINKKITLLLMEAIEVLIKRL
                     SIEDEDVIRYIKKYEKIFIESVLSSIKSNTFKSMVTVSKNKKEHLEDYIVDDESKLDD
                     ESFREIFNEILDSTSIIEKIRIIKENINAKKDFIDILESECLFGEEYLMLFASLSELE
                     LAILGSVVFYEDIRMREINILEFILNKKTETTLWKIEYIKFMKKQSEDKIYSIEEHMN
                     KIN"
     gene            complement(19267..19701)
                     /locus_tag="KRL67_001902"
     CDS             complement(19267..19701)
                     /locus_tag="KRL67_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH1522781.1"
                     /translation="MISPIILSSINRNLKEIERNELLETNIESGDYGLTLSESDVKDI
                     INSRDNTLKGYGRIELDIKVTKQLIENIYTSQYTNVDNYLETINDMQEIFYYLKNETD
                     DKICDDEIIEILGEFYEKFSGNMDNVRGEADEFAKKFKFGEV"
     gene            complement(19960..20871)
                     /locus_tag="KRL67_001903"
     CDS             complement(19960..20871)
                     /locus_tag="KRL67_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CGNR zinc finger domain-containing protein"
                     /protein_id="HBH1522782.1"
                     /translation="MNKKIELIGNFASDWIRYSDYEYKITEEDELYIVPTEDAVFTMY
                     NPFDVADDIIVDIIRIGQEALQDISKETKEMKLKKEVLEFVKKYGLLGLIYSSVYNRD
                     IVGEERVLMIEKNYITKENILSTDEYVNMFIPFASDEDVYFKKYRRGIDVSKREESPK
                     FFGKRPLILDLVFSKFYTEQLDWIVSFAKDMYKHFNQICVYKNTSNLLTESVTIMSGG
                     FNPQKIGFSISQLDKPIIAWEFDSLKTAIETIYAFAITSEPSSLNKCKYCGKIYIPTN
                     SKSQYCSPSCRNCSNVKKSRSRAKKDK"
BASE COUNT         6645 a         3751 c         2187 g         8441 t
ORIGIN      
        1 taagataata agtattttta ggattctatt acataatatt atttaaatat tcttctatat
       61 ttctcttctt cctcttcagt aaacacatta aatacaactt ttaaattttt ttcaggattt
      121 tcttttagcc ataatctaac tctacttaca gcaagattag ctgcttcttt ttttggatag
      181 ccaaatacac ctgtagaaat tccacaaaaa acaatatttt ttatttcatc aatttctttt
      241 attgtattta aacatgattt ataacaatga agcagttgct tttcttgctc tttacttggc
      301 tgaccaccag atacaatagg tccaacagta tgtactacga attttgcagg taaacaataa
      361 cctctagtta tcttagcatc tccagtatat tctaaatgcc cttgtttttt tattattttg
      421 tcacaatctt ctctaagtct aggacctgca catgaatgta tctcattatc cacacataga
      481 tgtaatggtt gtaaacaccc taataacttg ttatttgcag cgtttactat tgcatctgct
      541 cttaaattcg ttatattacc tctccatatg gcaatccctt cctctacttc ttctatatca
      601 tttacatcta ctaactctct ttcttcacat tcaatcaaca ataaattatg taacatatta
      661 agtatctctg gagcaatttc ttttggtgga tatacattca aaagtcctct aagtatatct
      721 ctaggcttaa tttcatcatt tgataaattg tatattttag aaggaacttc ttttgaaaaa
      781 tatgcaatta aagtgtttat attttttctt ctttgttcgt cagttagagg cttgatagtt
      841 ttatcaaaat cctcaaataa atttatatca tcagcataat ctctccactt catagccatc
      901 tctccattct tgtattttag acttaaaaat aattatgtat ttatgtacat atttatttat
      961 ttctataaac acctacataa atcctgttaa aagtgtaaat tttttattat gcaacttctc
     1021 gagttatttc tattgattga tagtaaatta ttgtacctaa atttaatcca actaacaagt
     1081 aactaaacct actttcctaa ctgcaaaata cactatgttt gccacttgta tctcatttgt
     1141 aaataaataa ggatactact tgtttaccaa gtacaaaact tataagtggt tgtactccta
     1201 gtgttaatcc aaataaagtc atatatatac taatacttat ataatttatt atactataag
     1261 ctacaattcc tattttcctc ataattttta ctaaaactac attgtgtatt aatgctataa
     1321 ataacagatt atttacaagt cttgaagaag tcagagtatc atggacaaac ttataaaatc
     1381 ataaataaag tccctacaaa tacattaact aagctatgta ctaaactgta ggaactttaa
     1441 aaaatatatt attcttaatt aagttctggc caataatttc tatttgcttc tattaaatca
     1501 tctagtaatt cttttgcaac cgttgcacta ggaactgtct tagatagcat aagtgcttgc
     1561 cacaatttat tatacgactt ctcaatccaa gcatcaacta ctaatttttc aactgctaat
     1621 tgttgttcta ttaaaccttt ttggaatgtt ggtattttcc ctattgataa tggttcatac
     1681 ccatcttttc caactataca tggaatttca accatagcat catcttgtat attttctata
     1741 gctccattat tttgaactat caataacata cgctcctgtg tattaaaagc tagagctgta
     1801 gctaaatcca ctataaactc tgcatgtatt cctatcttaa gcccactgcc cttggaatgt
     1861 ccttgctttt ctatcaatgc acatgaacca aaaacttctt tttctcttcc atcaataact
     1921 tcattggcac gagtataatt tacatcagaa tgatttacaa cataatctgg aaatagatag
     1981 tactttaagt aactacttgg tattgtagtt aaatctgttt ttactatttc tttagtaaat
     2041 aaattagttt caaaccaact atctttcttg tcatttttca ttccttcttc accatttata
     2101 aaaccatatt cttttatatg ttctactaat tttggtaaaa gttcatttcc ttttttatct
     2161 ttaattgaag tataccaacc aaagtgattt aatccaaagt atcttatatc catatgttta
     2221 cgagatttaa gccccgcaat ccttgctata ttatgttcca tacctattgg catatcacaa
     2281 atatttatta ccctagaatt aggtctcaac cttctaacag cttctgctat tattgatgct
     2341 ggattagaat aatttaacat ccaacaattt ggagaatact tttccatata gtctatgttt
     2401 tcaagaacac cttctataga cctcattcca taagctattc cacctggtcc acaagtttct
     2461 tgaccaacta ccccatgtct tagaggtatc ttttcatcta gttccctcat ttcaagtttt
     2521 ccaactctta tatgagctaa acaaaaatct acatctgtat aagcttcttt agggcatgtt
     2581 gttgctaaat actctatatt agggtccttt tcctttatta gtatttcaca agctttagct
     2641 accttatttt gtctttcttc atcattatca tataatttga ttgctcttaa tggtaaccta
     2701 tctaagttat ctagcaacat taatatgatt tctggggtat atgtacttcc tcctccagct
     2761 attactactg aaaatttttc cataataata tctccttttc tatttaaaaa tttactatat
     2821 ttataaataa atttataaat actagtactt taaagtaaaa gggagttcat ttattaaatc
     2881 tattaattta ataaactata gccaaacttc aagcactatt tacaagaaat ttaattctta
     2941 ttaattcttg ggtccatcat ctcaaatata tctacaagtt cagatactaa agaatgagca
     3001 aataatgcat ttgaataatg gtcttcagag tgcacaacta aaaaatcagt cccactatct
     3061 tcactattca tagcatttga tagtaattct aaatgatgct ttgatgctgt ttgtattttc
     3121 tctgaggctg aatttaaaag gtgttttgct ttattgtagt cacctttttt tatttcctta
     3181 agagacatca ttacatcact tttagcatct cctgcttctg atattatact aaatactttt
     3241 tcttccattt caggtaccat atccttctcc ttctaatgac ttgttaaaaa tcaaagctat
     3301 catcatctaa tgttgtagct aattctgcat ttttatttaa ttcttccttc tccattatct
     3361 taaagaatgg ataatatatc aaggtcataa gtattatact tgcaaatact aatacgattg
     3421 ccttccaatc taatgtcatc aaaaatgctg caacccctgg aggtaaaaag cctggtggtt
     3481 ctgcaatagg taatcctact aatcctactt ttaatgccat aaaattaagt gctccaacca
     3541 atacaggtcc aaatacaaat ggtacaaaca tatatgggtt cataactatt ggtgttccaa
     3601 atattactgg ctcatttatt ccaaatagtg atggtaaaat cacaactttt cccatactat
     3661 taaaactctt acacttactt cttgacataa ggtacactaa tcccaaagtt attcctgaac
     3721 cagttacttg caagaaacca tagtaaaaac ctattgtaaa cacatatttt gggtctcctc
     3781 ctgctgctat taacgttgca ttttctgcca tatatgtagc tgctattggc atccatatac
     3841 ttgaagttat tgctgctcca tgaagcccaa agaaccataa taattgagtt ataaatagta
     3901 ttatagttac tgcaaaaaca ttatctacac tgcttatagc tggtgctaag aaattcatta
     3961 taatagctgg tagtggttga cctgagacat ttttagttat tgctgctatt gctgtaaata
     4021 acattataca tactatcatt ggagttaaat ttctaaaact tttatttact acactaggta
     4081 cttcttttgg aaatctaatt acgagatttt tatcattcat aaacttagta atatcaacta
     4141 ctaaaagtgc tactaatata cttacaaata aaccttgtgc atctaaataa cttgtatcta
     4201 atacaccatc tactacttgt ccacatacaa caagttgtgc aacaactgct gtcataccta
     4261 tttctacatg attaagatta tattttttag ctagtgcctg agctataaaa aatgctgaat
     4321 atatagataa taaactcata gtaaatttat tagggataag taatgtatcc atattttcag
     4381 ataataatgt ttttgcactt ccttcaggta acatattgtt taatgccata aataacatac
     4441 ttaaagaacc aacaattatt attggggtaa tgcttatcat accttctttt atagcttgaa
     4501 gatgcctttg tttttcaatc ttttgtgcta ttggaagaag aaattttctt aaattatttt
     4561 gaagtgcttc catttttatt ccccccaatt atctattttt atataattcg attgccgact
     4621 tcataacaga aggagcatcc atacgaccat aatgcttagt atctattaaa ccaactgcta
     4681 ttttgtaatt cttacaagct tcaactacat catcttcctt aaacctaatt tgtggtgcta
     4741 caagtactac atctgtatca ttagcttcaa tgtatgattt taaattttca aatgatgttg
     4801 cctctatatt tatagtttta gtttttaact tttcatgctc actacaaaaa cttttcatct
     4861 tttccacaag tacacctgtt gaagctccat ttgcacatac taataaaata ttcataatta
     4921 ccctcctaca atatctaacc tcttttgttt tctaattttt tatcagccaa ttcttactct
     4981 aattcctatc agtttttact ttaattctta ttactttatt ttaattctta ttaatattta
     5041 ctctaattct tagtactgcc aatgattaaa cttgaaagct gtatctataa cttcttcttg
     5101 cttatatttt tctaatattt ttaagtatct ttctttataa tcattatcta cttctttttc
     5161 tggtattact atactagctt catttaaaaa gtggaatgaa tcccttccca tttctaatcc
     5221 tctatatgta tgcttgtcaa gtgccacgtc cataacttct ggaactttac ccattgcaaa
     5281 tccttttata attatatttt ttaataaatc acttgatcta tctttctcac aagcacaaag
     5341 atatcttatt gcatgtatga aaaataaagg tctgtctcca tctgcatatg aatattcctt
     5401 tcttacttga tttaaattat ttatgattat tacagctgat gggtcaccca ttcctatatc
     5461 ttcaactgat attgtaagta ttcttctcca tagcttatct tctaattctg gtgatgatat
     5521 atatagttca taagcaaatt cacaagcttc ttccaccata tttcttctta tagctttttg
     5581 aaatgacgat attatttcgt ctgcactaaa tccgtttctt gtagtcattc ttgcccatgg
     5641 gtcatttgcc tttttatctc ttaatatttt atcttgcata ttatttactc ctttttattt
     5701 aaattttaat tatttatatt actcatttaa taatataagc aagtattatg ccagtttttt
     5761 tagacacttt aaaacactgt tatttcaatc atttttatca tcaaaactta gatactttgt
     5821 ctaagtatat ttatatgtgt ctaatattaa tattatttta gactaaaaaa aattaccaaa
     5881 taagttttta tactcattgg taatcttttt cgattatttc tgcatattct aatattaata
     5941 atatttgata gcactctttt tcattaattt ctatattata ttttttatct aattgagata
     6001 ctatatcttt tattttgctt aggctcttgt gataatcaag tgttttttct ttatcaagtt
     6061 tttcaatttc tctagtattt ccaatcaacc catctaccat gaaagctaaa tgcatcataa
     6121 gcccaattac tttatcgtat ttcttttcta tattaaatac taaaaacaat tcatcagaca
     6181 catattttat atctttaaat atcctattat aattagagct ttttaaggat gtctttaaac
     6241 cttcaactac atcatcataa cctatactta tctttataat attttttaat ttattgatat
     6301 tttcatcctt atctatatct tttttgctaa tatagtcaat tcctctaata ttaattcttt
     6361 gttcattgat tactgcaaca atattactac tttctttaag ttttgtaata tttaaactta
     6421 aatctttttc tgttttatat gggacagata taatatctat tccatctaag tacctactca
     6481 tcttttcttc taatattttt cttgttttat tatctacacc ttctgaaaca tgtgatgcta
     6541 taataattgt tttagacaaa ttttctttat tttgaatttc attagttcca aaatagtact
     6601 ttctcgtgtt agtgatagag ttgtatacat catttaaagg taatcccata ttggccttct
     6661 gaactgcttc taacacagtt aatgtcgtaa ccataggaat tactcttact ccacactttt
     6721 tttctttctt tataatttca tcaaaggagt ttaatgaacc catatctgta aatagtaata
     6781 catctttatt gtctatatta ttaattaaat ttttaaaatc tacaatcact tcatcataac
     6841 tcttatctaa cttcatatcg aaagaaatag cgcttcactt tccaataata tcaccaactg
     6901 ctttaaccat actacttgct gtagtttctc catgcatagc aactataata ttaatgttat
     6961 ttactatttt attttgttca tcttgtttag cttgtactat gaataatgta atatatgcta
     7021 cagtttctat agataaaaat aaatcaaatt cattttcaat tattccaact atttttaatg
     7081 ctgttttaaa ttcacttctg ttatttcttc taatttgatt caaatctatg ttgtttgtag
     7141 agctactttt atccaatacg ctttttatat aaattgccat tgacttaaat attgattcac
     7201 ttatatctat ttcttcatct tctttaataa tttgtcttat tgattcacac atgcttttaa
     7261 acttaacatt attaaataga gaagcatctg ctatattcat ttctacagat ttattaagat
     7321 tagtatttac tgcattaata atatttatat cattgtagtt aataacttct tcttctccta
     7381 atgacgtaaa tacaaaatat tctattccat gtgttataac ttcatctgct ttaatagact
     7441 tctccctata atcatcactc acttcattta tagatttatc aaaatatttt ttctctatat
     7501 aaactggttc tttattatct cttttatatc ttaggtatcc atttgcaaca gctaatttta
     7561 tatcattttg aagttgacct acattatttt tacaatcata aagtaatagt gatttaatta
     7621 tttctttatg tacttttatt gaaatagaaa tactttttga ttcttccttg aaaaaatttt
     7681 tgactaattc cattctttca atcattgtcc tatctctcag tgatggtatt tctattttca
     7741 ttggtattct tctattgaat gtatctaata gtgttgatgt cggattttct gtagtagcac
     7801 atattattaa taccttagac tctctttctt tcgttgcatc tcctagtctt ttaaatgttc
     7861 ccttatctat aaaagtaaac aacatttctt gaccttctgc tggtagtcta tgaatttcat
     7921 ctaaaaatag tatccctcca tttgcaattt ccaaaagtcc ttttctggaa gattctgcac
     7981 ctgtataagc tccttgttct actccaaaaa tatgtcccat aagtaattgt gcattattag
     8041 cataatcagc acaattaaat gttacaaaag gagctttatt ttttctaatt ccactatcta
     8101 tagaataatc gtacatatat ctagcaatca tactctttcc tactcctgtt tctccataga
     8161 taatagtatg aagtccattc gtaggataga gcattgcaac cttgcatttt tctattatag
     8221 gtcttaaact atgttcttta cctattagtg tttcaaaact atcttttttt attttgtttt
     8281 ctacttttgt tccttttttt attccaagca actcactttg cgtatcaagc aattcattct
     8341 tcttaaggtt aagatttgca gggatataat acacaggtct tccttttatc tttgtaacca
     8401 taccctcatt gaataactgg tttaaatatg tacttatatt gtttcttgca attcctgtct
     8461 tttctgataa ttggttagct gtaatccctt tttcattttc cctatatgat gattctaaaa
     8521 ctttcattac tttatctttc ttatatatca ttacaagctc ccctacatta taatatttta
     8581 ttaactaaaa cccctataca cactttattt tttaataaac tcttattctt taactaaggt
     8641 tataatttat caaactttcc ttactttaaa ttatagaaaa ttattgtaga aaaaataagg
     8701 ttattgctca ttttttctac attactattt ttaaatttat agtactttct taataacata
     8761 ctttacttgt ttttaatttt attatatttg aatttattat atttgaaaag tagaaataaa
     8821 aaaccctatt tgggagtagt tttaaaactt ctccaaaaat agggttttag tttgtatttc
     8881 aaataaatac atgttcaagt atttttatac taaacaactt ttaaattttg ttggtattta
     8941 tttatgtctg atttagaaga atatacaaaa tgtccatctg aaatctcaat ccaacttaat
     9001 gacttttcat ttgaacaatc atatacatta gaattatact ctattctatt tcttgcctta
     9061 gcatatctag ggtcaggaac aggaattgca gaaagtagtg actttgtata tggatgaata
     9121 ggattattat acaattcatt tgaatctgcc aattctacta attttccatt atacataact
     9181 cctatacggt cactaatatg tctcaccata gataaatcat gagctataaa taaacaagtt
     9241 aaattctttt cttgttgcaa ttttttaaat aagtttacta tttgcgcttg tatagacaca
     9301 tccaaagaag ctataggttc atcagctatt ataaattctg ggtctactga aagagctcgt
     9361 gctatgccaa tacgctgtct ttgtccacct gaaaaatcag atggatactt atttgcataa
     9421 cttctatcaa gtcctactag atttagcatc tcatatactt tatccattct atctgtttta
     9481 tttttacaga taccttgaat ttttagtggc tctgaaatta tatctgcaat tgtcatacgt
     9541 ggatttaatg atgaagtaga atcttgaaaa ataatctgca tacttttgtt tacatctttt
     9601 tttataaatt tgtacgtttt tttatctgat atacagtttc ctttgtaaat tacttctcca
     9661 ccagtagatt catgtagtct tattatagtt ttaccagttg tagattttcc tgaacctgac
     9721 tctcctacca atccaaatat ctcaccctta tatatatcaa aacttatatc atcaactgct
     9781 tttacagtag tacttttgtc caaatgaaaa tattgcttta agttttttat ttccagtatt
     9841 ttttctctat tcattagaaa tcacccttcc acaatttact ctaactggta catctacttc
     9901 aggtgcatct gggtgcaata accaagttgc tgcactatgt gtatcattaa ttttaaacat
     9961 tggtggctct ttttcataat ctatcttaag tgcattttta ttccttattg caaaagcatc
    10021 tccttttggt gggttcaata aatttggagg cattccaggt atattatata aatagtcatc
    10081 ctgcgaatct aaagtaggta atgaacctaa caacccccaa gtatatggat gtctagggtc
    10141 ataaaatata tcctctacag ttcctatttc tacaatcttt cctgcataca taactgctat
    10201 tctatcagct atagttgcaa ctactcctaa atcatgagtt ataaatatta ttgacaaacc
    10261 tattttatgt tgtaaatcct ttattaaatc aattatttga gcttgtatag ttacatctaa
    10321 agcggttgta ggttcatcag caatcaatac atctggatta caggcaagtg ctatagctat
    10381 tactatacgt tgtctcattc cacctgaaaa atgatgtgga aattgtttaa atcttttctc
    10441 aggattatct attccaacca attcaataag ctctatggct ctcttttttg cttcactttt
    10501 acttatacct tgatgaatga ttacagcttc tgcaatttgc tttcctattg atatagttgg
    10561 attgagagat gtcatagggt cttgaaatat cattgatata ttctttcctc ttatatcttc
    10621 catctctttt tcagacttct taactaaatc acctgattta agtaaaacct ctccattttt
    10681 aatatatcca ttgtatggga gtatcctcat caaactctta cacagtacag atttcccaca
    10741 accagattct ccaactatag ccaatgtctc accttttttc agattaaaag atacatttct
    10801 tacagcctga acttctcctt cttctacttt aaaacttaca gatagattat taacctccaa
    10861 taaatgttcc aaaaaccatc aacccctatg ctattcaaac cttagggata tatttttatc
    10921 cctaaggttt ctaattaatt tcaatattat tcaattgtcc agtcagctat gttccagaat
    10981 atacctacac catgatgacc taaaactgta tcaggtgtta atccttttat gtttggtttt
    11041 ccaacatata ttgcatctat ataagcaata aatgtataag gcatatcctt agtcatttct
    11101 acttggaatt gtttgtataa ctttaatttt tcatctttat cttctgtttc tcttgctttt
    11161 tgaagtattt tatctatagt aggattagag taagcactgt aatttgcacc tttatctgtt
    11221 ccaaatactt tatatgtatg gtcatctggg tcaaatggac ttccccaacc tattaaatga
    11281 gcatcttgat tagcccaatc tgtttcagtt acaacaacag ctttagcatc aacaccaatt
    11341 tcttttaact gttgtgcaca tattttagcc atatcaactc taacttggtc actctcacct
    11401 gctgttatct cgaaagctaa cttagtacct tctttttcat agattccatc agaacccagt
    11461 ttccaaccta gcttttcaat ttcttgttta gctttttctg gattatattc aaacttttct
    11521 atatcaggat tattatatgg tcccatttgt aaaggtgaat atgctggtac accatgtcct
    11581 aataatacac tgtcaactat tgcttttcta tctatcgcat agcttaaagc atttggcaat
    11641 cctttagctt ttttatcttt aaagaattta gaattaaaat tgtataatat acctctataa
    11701 tctgctgttt tcattatatt tactttaaaa ttcttttcgt ctttttcaaa gttagacata
    11761 tctttaggag ttacttgggc taaatctaat tctcctgatt ttaattgcat tgccttagcc
    11821 ttatcatctg gaacaatctt aaagactact ttatctaatc ctggttcttt tacaaaataa
    11881 tcactattct taacaagtgt tatactttga cctttgtccc atttttcaag tttaaatggt
    11941 ccagtaccta ttggtttttg attaaattca tcagtagcta tatccttacc ttccaaagca
    12001 tgttttggta atattcctac tgttaaataa tcaagcattg ctgtatttgg agcttttaaa
    12061 gtaattttta tagtattatc attaaccaca tcaatttttg ttatatcttc atagtttgat
    12121 gctatctcag aagcattatc tggattcatt atagtttcta ttgtaaactt aacatcattt
    12181 gctgtaaatt tctctccatc atgccactta acatcatctc ttaaattaaa agtatatgta
    12241 tttgttgcct catcaaattt ccagtccttt gctagacaag gaaccacctt attattttca
    12301 tcatgagctg ttaatccatt aaatattaat gaatttattt ctccatgttc atatagtgca
    12361 ggatttatac ttgtatagtc attacttcca taaacaagca cctttccatc tttaggcgta
    12421 tctgccttct tatctttatc tcctccacta gaacaacctg ccattagact aagtatcatt
    12481 actaaactta aaacttttaa cttctttaat ttcatgatgc tccccctttt gctttttata
    12541 aaatattttt caaagctttg accccattta tttaaataga tatttcaaat acctcagtat
    12601 ttgcttattt agtactcaaa tgctaattta taaattacta gattttttat tattattctt
    12661 tcttatataa ttacctatat tcgttataca caccaatgtg gtaaccaaaa atatacccgg
    12721 aatcagtata atccaccatc tatttgaaag taatgcttcc tctgacaaag ataacatact
    12781 tccccaagaa acaatttcta ttggtaaacc aattcctaaa aagctaagtg tcgattcagt
    12841 accaatagct gcgcctatgt ttgttacaac catgaacatt attgatgcca caaaatttgg
    12901 aagtaaatgt tgctttaata catagaagaa tcctcctccc atgctcttag ctgctagtat
    12961 gtattcgcta tttcttattt gtctaacttc tgttctcact atcttagata tattcatcca
    13021 acttgttatc cctattacta tggacataga tactggattt gaatttccaa gtatagcttg
    13081 tacaaatatt ataattaata ttgaaggtat actaagtatg atttctgtaa acctcatcat
    13141 tgcatcatct acaagttcag atgctgaacc acttatacta ccataaacaa ttcctataac
    13201 agttgatatt acagtggaaa atagtcctat aaataaagat atttttcctc cataccatat
    13261 catagaatat atatctctac ccattgagtc tgttccaaat aaaaaagttt tatttggagc
    13321 taaattagaa cttgctaaat ccatgtatgt tggttcatgt gtcattataa aagatgagaa
    13381 tacacttcca atgactatga ttgataaaat aataattgat atatatggta attctttaat
    13441 ttttttagat aaggttttct ttgtttctat atgttcatct tctgctacta aaatataatt
    13501 ttctcctaca atttcaaaat cactatattg tggcttcatt agtatcacct ctatcatatt
    13561 tcattctagg gtctatttta ttgtttataa tttgagctaa catattccca aatactacta
    13621 aagcccctgt tattaagcat aaaactaata acatattata atcatgatac tttgcacttt
    13681 caaaacaaag tgttcctagc ccaggataag aaaaaacttt ttcaactaca taagttcctc
    13741 ctagtatatg aggtatcgaa atggccatta tactaatata tgatggcatt atatttctca
    13801 ggcaatgctt aaaaacaata gttctattat ttaaaccttt tgctttacat aaaagcacat
    13861 aatcttctct tatttcctct agtaatttat ttcttatcat atatgtgtaa taccacaaat
    13921 ggcttaatat aagtactgta agaggtaaga ttagatgtga tatcctactt aatatactgg
    13981 actcttctcc cattgaataa gcaccactac ttggaagtat gcttaagttt atactaaata
    14041 taagtatgag tactaaggca acccaaaatg atggaataca attggttatt gttcctaatt
    14101 tgcatatgat tctatcaatg agcttatctt catgtaatgt acaaaatatt cctaacaata
    14161 aggctaacac aaaggtaagt atatatcctg aaccgcctaa tattatagtg tttatataga
    14221 catcatttat aacagaagta acattttgtt tatatttaaa tgaaatccca aattcccctt
    14281 ttgatgcatt tgaaatccat tttatatatt gactgtatat tggttcatcc aaaccaagtt
    14341 ttttcatagc attttctttt tcttgtgtac tcattctttc tactccatcc ccatagtaag
    14401 agattaatgg gtctcctgga gcaagtcttg ccatgtaaaa aacaataaaa gataataaaa
    14461 acattactaa aataaactgc aacaattttt tcagtatatc ctttaacaaa ccccaacccc
    14521 cctttgttgc aataaaaaaa accacataaa agcaaacgta aagtaacgtt aaaaccttcg
    14581 tggttccatt atcattaaac aaaattaaat tttcaatatt tatctatgag ttaattttag
    14641 catcatttat taactgtgta aacaaacttt ttaaaaattg ttaaaatttc atcaaattat
    14701 tttatttata cctaaaataa aatttttata ttctatcctt cattattttc tataaaatta
    14761 taaaatttta ggttgactat ataagaaaag tatattatat tagtttgtat gcaaaccatt
    14821 taagaggtga ttttattgaa aggtaattat gactgtttta gaatagccat gctcttaaaa
    14881 gaactttatt ctaaaactat gtacacagta gaagaaaatt ttaaagaaaa tggattaaca
    14941 caccagcaga ttatagttat aaaactgata gctcataatc aagagcttac aatatcacaa
    15001 ctatgtgatg aaatgtcttt agctaaaggt acggtttcag gcataataag cagattagaa
    15061 cagattggat atatagaaaa attcaagaaa tctaatgata aaagaaatac atacgttaag
    15121 tttacaacaa caggatttga atttgccaca aattttaaaa ttaaaatgca agaaagtttt
    15181 gatgatattt ttaaaaattg tgatgaaaac gaattgagtg acttagtaaa aaatcttaga
    15241 aatatattgg caaaagtaaa ggagagataa ttatgaatta taaattaata cttgcaatag
    15301 tatttataac atcagcttta attttctaca ctataggtgt atttggagaa agaaaggcca
    15361 aaattttaaa aaagaaacat gtaattatat tttggcttgg ttttatattt gacactttag
    15421 gaacattcac aatgagtaac atagccaaca gccatacttt tgaagtaaaa tctgctttat
    15481 cacaaaacct tcatagtatt acaggtcttc ttgcaattgt gcttatgctt tttcatgcaa
    15541 gttgggctac ttttgtccta tacaaagatg atgaagagaa gaaaaaattc tttcataagt
    15601 ttagtatagt tgtatggact atatggttag ttccatactt tataggtatg tttattggta
    15661 tggctggata gtaaaataaa aagtgtgtaa gaaatactgt aggtaaattt gtataagaca
    15721 aatttatcta catccttctt tcacactttt acatatttat ctataatact ccattatttc
    15781 tttcatattt cttaatacct atatagaata tttaattgtt tctatagtat tttattattt
    15841 tctcacattt tttcatatgt atatatgaaa tatttcatta tttctataat gttttattat
    15901 ctttctcata ctttttcata tatatattgc ttcactattt tttttatact tctttattaa
    15961 atacttatgt ataccataca tttaataaac tctaactatt aaacacctaa tacttgagtt
    16021 actcctggaa cttcactcac caaaacattt tcaataatag ctttgattgt catagttgct
    16081 cctggacacc cactacatgc cccttgtagt ctaactaaaa ctacaccatt ttcatttaca
    16141 tctataagct ctacatctcc accatctctt tgtaatactg gttttatctt ttcctcaagc
    16201 actttttcaa ctttttctct catagctatt ccccccagaa atattttatt accaaaatta
    16261 taccaatggt ttaaattttt gtccatctaa tgatttatca aattcaattt ttgcttcatc
    16321 aataatttta gtatccataa atacatcata agcactacaa gccagtactt tagctgaatt
    16381 tatcatagca gaaaatccta tatttgaacc tgctgatgaa catgactgcc atgtatgact
    16441 tgcaattcca acaggccacg ctgccattgc aaattgagcc actggtatta cataacttac
    16501 atctccaaca tcagtagaac cagctagtcc tttatctttg tcaccgtcaa ctatttcctt
    16561 atgtatatag ctttctacaa tagatttatc attttgtaat gatgttgcta cacctaatct
    16621 ttcttctcta gtaagagtat cacacaactt ttttgcaaat tcttcttctt caggagtatt
    16681 tttagggcaa ccaacaaaaa ccatattgtc ataaactaaa tcagtcaaac attggtttgg
    16741 tatatagtca tatattccat ctgtaacctt atactccatt tcagtttctg tcatcattgc
    16801 tgctccttga gctactttta ttaatctatc aagaacatgt tctgcatcct ttgctttttt
    16861 acctctaata aagtaccaac tttcagcaaa tccaggaact acattaggtc ttccaccacc
    16921 attagttata acatagtgca ttcttataga gtcaataaca tgttctctca aataattagc
    16981 tccaacattc ataatttcaa cagcatcaag tgcacttcta ccgttatgag gagcttgagc
    17041 tgcatgggct gttattcctt taaatttaaa ttttacagat aagtttgcca gacttccacc
    17101 tctccaaggt gcatttatat caaaaggatg ccaagtaaat gcacaatcta catcatcaaa
    17161 acagccattt atacacataa ctgtctttcc tccaccttct tcttcagcag gacaaccaaa
    17221 atattttatt gttccattaa atttattttc ttctattaat ttttttatag aaacaactgc
    17281 tcctacacct gctaccccca acaggttatg cccacatcca tgtccagctt ttccttctac
    17341 caatggcttt ctttctgcac tcacacattg tgatagctct ggtaaagcat catattctcc
    17401 aagtatgcca atcacaggtt taccctctcc aaaacttgct ataaatccag taggtaaatt
    17461 ttctacttct tcaattttaa atccttttga ctttaggtat tctttttgga tgctgcttgc
    17521 atacttttct tcaaaggcta attctggatt ttcccatatt ttcttagata aatctattaa
    17581 ttcttctctt atttcttcaa cattatttat gatatactct ttcataatct tcctccaaat
    17641 atatgtattt ttgatatgct ttacaaatta tattatatac taacattcta tttattcaca
    17701 attttttgtt aattaatttt attcatatgt tcttctatgg agtatatctt atcttcactt
    17761 tgcttcttca taaatttaat gtactctatc ttccataaag tagtttcagt ttttttattt
    17821 aatataaatt ctaatatatt aatttctctc attctaatat cttcataaaa tactacacta
    17881 cccaatattg ccaattctaa ttcacttaat gatgcaaaca acattaaata ctcttctcca
    17941 aataaacatt cagattcaag aatatcgata aagtctttct tagcattgat attttccttt
    18001 attattctta ttttttcaat tatagatgta ctatctaata tctcattgaa tatctctcta
    18061 aacgattcat catccaattt actttcatca tcaacaatat aatcttctaa gtgttccttt
    18121 ttatttttac tcacagttac catactttta aaagtgttac tttttattga gcttagcaca
    18181 gattcaataa atatcttttc atatttttta atatatctta ttacatcttc atcttctatg
    18241 cttaatcttt ttattaatac ttcaattgct tccataagta gtaaagttat ttttttattt
    18301 attctaattt ctctgttaat ttcttcaaaa tcattataat ccaaattact atctacttca
    18361 gtgttaaaac tatcatctac ttcaatcaat tcaattatat tactttcttc agagcctata
    18421 actttatctt cagtaatatt attgtataaa tttttaaatt gtttttctaa aaattcatac
    18481 tcatattttg aaatttctaa attagtcact tcatttccta aaatagtaga aaaaatacaa
    18541 ttatttataa taagttcaaa taaatttaca agtataacat cacaatctat tttataagtc
    18601 actccatagt cataaaataa tttttctcta tcttttttct taaataagtt acaaaatcta
    18661 ttttctatat ctatactctc aatataattc ttcacataat aaataccctt aacatccata
    18721 tcatctagtg ccaatggata atctatactt actggaacgt cctgagcttc aaattttata
    18781 atataacttt taaagaaact tgataactcc atcaaggtat cattatatgc tattgaatta
    18841 gtattcaatt ttctactcac cattagttca taaaaatctt ttagtctaac taattcttcc
    18901 tctaatattt caatacctcc aaggtatact tttttaacat tcttagcagt caactccaca
    18961 actctttttt ctatatctcc aagcttattt atatatgcat caatagcata ccatattgaa
    19021 atcaacagtt tttcggcaac ttctgttttt actgatgtgc tttggccatt tgtatatttt
    19081 ttaatcaaat ccataagtat tagagatatt tcttgttgta tattatatac ttctctttcc
    19141 gctatcactt tacaatttag acattcctta agtagagata tggaatattg gttctttatt
    19201 aaattatttt cgtttatatt aatattactt aaattattta acatacaaaa tctctcctgt
    19261 catatattaa acttctccaa atttaaattt ctttgcaaat tcgtctgctt ctcctcttac
    19321 attgtccata ttacctgaaa acttttcata aaattcaccc agtatttcta taatctcatc
    19381 atcacatatc ttgtcatcag tttcattttt taaataataa aaaatttctt gcatatcatt
    19441 aatagtctct aaataattgt ccacatttgt atattgtgat gtatatatat tttctattaa
    19501 ttgttttgta acttttatat ctaattctat tcttccatac ccctttaaag tattatctct
    19561 cgaatttatt atatctttta catcagattc agaaagagtc agaccataat ctccagattc
    19621 tatattagtc tcgagcagtt catttctttc tatttccttt aaattccgat ttatagaact
    19681 caaaataata ggtgatatca tttttaaatt ctcctttttt attttttcta ttgacttttt
    19741 ttgtttttta tgctatactt aaattaatat acattattcc actaaccact tataatagta
    19801 gtgttaaaag tagtttttgt caactcaaaa ttactatttc aattaaactt ttcaattttc
    19861 tcaacaaaaa ataccatcat ttagatatat aattttctct aacaactttt aattcagtgg
    19921 ttatttacta aaatctaaac aatgatatat ttattaattt tatttatctt ttttggctct
    19981 acttctactt ttctttacat ttgaacaatt tctacaagaa ggtgaacagt attgagattt
    20041 agaatttgta ggaatataaa ttttaccaca gtacttgcat ttatttaaag aagaaggttc
    20101 acttgtaatt gcaaacgcat agatagtctc aatagctgtt ttcaaagaat caaactccca
    20161 tgcaataatt ggtttatcta attggcttat agaaaatcct atcttttgag gattaaaccc
    20221 tccagacata atagtaacac tttcagttaa tagattagat gtatttttat atacacaaat
    20281 ttgattaaag tgcttataca tatctttagc aaagcttaca atccaatcta gttgttcagt
    20341 gtagaacttt gaaaatacta aatctaaaat caatggtctc tttccaaaaa actttggaga
    20401 ttcctctctc tttgatacat ctatacctct tctatacttt ttaaaataca catcttcatc
    20461 acttgcaaat ggtataaaca tgtttacata ttcatccgta ctcaatatat tctcttttgt
    20521 aatataattc ttttcaatca tcagtactct ttcttctcca actatatctc tattatatac
    20581 acttgaatat attaaaccca ataatccata cttttttaca aattcaagta cttctttctt
    20641 tagtttcatt tcttttgtct cttttgatat atcttgtaat gcctcttgtc ctatgcgaat
    20701 tatatctact attatatcat ctgctacatc aaatgggttg tacatggtaa acacagcatc
    20761 ttctgttgga actatatata attcatcttc ttcagtaatt ttatattcat aatcactata
    20821 cctaatccaa tcacttgcaa aattacctat taattctatt tttttattca tgataatcat
    20881 tcaccccttt caatgtaata tataatttta tattactatt acatttttag tttattccat
    20941 attttctata ctgtcaacta ttctttttat atttttagca tatcaaaatt tacaacagcc
    21001 tttataatgg acttaaatag ctaa
//
LOCUS       DAEPXF010000062        20986 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain HB27
            SAMN13376389-rid12479423.denovo.066, whole genome shotgun sequence.
ACCESSION   DAEPXF010000062 DAEPXF010000000
VERSION     DAEPXF010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13376389
            Sequence Read Archive: SRR10518432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 20986)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20986)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001069624.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001069624.1
            Genome Coverage       :: 315x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/15/2021 01:54:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,165
            CDSs (total)                      :: 4,076
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 89
            rRNAs                             :: 10, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 10 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 19 of 69
            Pseudo Genes (incomplete)         :: 44 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 10 of 69
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20986
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13376389-rid12479423.denovo.066"
                     /strain="HB27"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="China: Hebei"
                     /collection_date="2017"
     gene            76..897
                     /locus_tag="KRL67_001904"
     CDS             76..897
                     /locus_tag="KRL67_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HBH1522783.1"
                     /translation="MIKKEISNFRGSSDYVVSPELMASVNVAIALEKPLLIKGEPGTG
                     KTMLAQAISNELKKDLVIWNIKSTTKAQEGLYVYDTVQRLYDSQFGGEGVDNISKYIK
                     YGKLGEAFSSNQQVILLIDEIDKADLEFPNDLLWELDKMEFYINETKETVRAKQRPIV
                     IITSNAEKELPDAFLRRCIFHYIEFPDRDMMEEIVKVHFDKVEEHLLEQVMTTFYWIR
                     SLKDIQKKPSTSELIDWIQALTLSGMPIEKIEKEVPFAGILLKNNEDIESMQRHL"
     gene            918..2114
                     /locus_tag="KRL67_001905"
     CDS             918..2114
                     /locus_tag="KRL67_001905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HBH1522784.1"
                     /translation="MFIEFFYLLRSRGLNVSLNEWMTLIEALDKGLCYSNFSNFYYLC
                     RMILIKSESDFDKFDAVFLEYFKGIEHQEEIPEEIMNWLKKPDIDLEEFERLEQNPNI
                     NLDKLRAKLEERIKEQDSPHNGGNYWIGTGGTSELGHSGKGQTGIRIGGNSTYGRAVL
                     EVAGERKYRDFRDDEVLNMRQFQVALKRLRQFSTRIDAPKTELDLDKTIEETCNNAGY
                     LKLFFEKPRKNTVKLLLLMDSGGSMRGYSTLCNTLFQSVSKSNHFKDVKIYYFHNCIY
                     DRLFTTPECWLSKSINTEWILKNIDKNYKVIIVGDASMSPSELLHIGGNYRGPYNYTP
                     GIEWLKRFKRKYSKIVWMNPELRDGWDSINYWYQTQRMIQGEFNMFPLTVKGLEKALK
                     NLMVSR"
     gene            2281..5679
                     /locus_tag="KRL67_001906"
     CDS             2281..5679
                     /locus_tag="KRL67_001906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HBH1522785.1"
                     /translation="MIIINYELIVKYNGDILRLEEELGVSVEILNSSYAIITSSNEED
                     VNTLLTYPEIEFIEKPFILQTQDVQSFSSTGITGFKNRTGLTGKGTIIGIIDSGIDYT
                     LPVFRDSDGRSKILYYWDQSIQGNPPEGFREGTLYTNEDINNAIDGSMYIPISTTSLH
                     GTHVAGICATIASDARIIVVRVGNIQTDIFSRSTEFMRAIKFILDRALELRMPVTLNI
                     SYGSNEGSHRGTSLFEQYIDDMCLFWKNNIVVAAGNNADKGGHKRIRLQNNITEEVEF
                     IVGEGERILNINIWPDFVDDFSVHLVNPSNNQTQAISLTSGEIRNTLGETRITGYFYP
                     IAPYSLTRRVTLQLSSNTQITPGLWKIVFEPIDIVTGNVNIYLPTSEGLNRNTRFLIP
                     TQELTVTVPGTASRVITVGSFNSRTDIVSIFSGEGDTQLGVFKPDLLAPGEDIVSFLP
                     GGTSGALTGTSMATPHVTGVCSLFMEWGIVNGNDLFLYSQKLRALLLKGARRLSNQSY
                     PNNSSGFGFLNLSDIDLYTLSNINQDLETEDIGYRSINKSFKDEENSYKFIDGYNMQI
                     HNDLENEIYISKNASRQSGILSGIDVVHTPEFEEELAGLGMGQNFFKISDSLGVLFIN
                     NTDYNSIQRVLQLPSIIRTVSTTKMTLLGEINRGTFGGVVATEEMGVNFFKNNPNINI
                     TGRGTLISIADTGIDYLHPDFIYPDGTSKIVYLWDQTKEGTPPDGFYIGTEYTREDIN
                     RAIAENDPSLSQDEVGQGTMLSGICSGLGNVNSEYAGIAEDSELIIIKLGKIDGFYNS
                     AMLFAASQYAYKKAFELGRPLVINMSLGTSSLAGLTNRSNSEKAFFTRGLCITAGAGN
                     EGNTQTHTSGIIPYVGGSVEVELELNEEEEELSLELWLNRPDKADVIIVSPTGEESKS
                     VGVSNYNKVTGLFDLEGTEYSITYIYPTTFSGQQFTNVTLKNAKRGVWKIRLVGVYII
                     TGRYNLYLPNRELLKSGTRFREVDPFYTINYPAIQDDLITIGAYNTINGSLWQSSSRG
                     PTIEDRLKPDIVAPGVNIIAAYPGNTYATITGTAAASAHAAGAAAMYFQYTFVDGRYP
                     NQAYVQKIKTFMQAGARKDSNTVYPNTNSGYGLLDVRGMFDVLR"
     gene            5689..7362
                     /locus_tag="KRL67_001907"
     CDS             5689..7362
                     /locus_tag="KRL67_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family peptidase"
                     /protein_id="HBH1522786.1"
                     /translation="MEKSYCIIYQGNIESALQENGINKYMVLNSQLAVIYVPIDFDET
                     ILNNIIQVAWWEESEPMSSLIEITNNVNNGETITTAAETDYIYENPYNDITGRGILLA
                     VIDSGIDYLHPDFINDDGSSKVLYLWDQEANTNPPPEGFIFGSEFTRSELNIAINRND
                     GSLSQDNIGTGTLVSGILAGNGRVNSQYRGITTESDLIVVKLKSYTDTYYAGRINYSV
                     SDFLAAITYVTNIARTENKPLIINLTIGVKSGAVATTSILDTFNILSSAGVVVVSGAG
                     NQGNTDIHYSGRFNSVGEVQDVIIQDGDDYALDITLNTNGPDKIGAQIISPSGEVSHD
                     IRYSPDFYIYRGKFNLENTTYAMRFIYPYITSGKENLEIRLRDIKPGVWILRLTSELI
                     IGGEYDIYLPNKNLIAPDTRFLDPDSVATITMYAASDDVITVGTFNNKTDSMWIGSSK
                     GPIRGRGIKPDIVASGVDIISTYKNGAYNTGTGTGVSSSIVTGVLALLMEYLEKQDNV
                     PRLSLFTQVLKTYLILGATKLEIYTYPNVSQGYGILNLKNTIQQIANTL"
     gene            7636..7830
                     /locus_tag="KRL67_001908"
     CDS             7636..7830
                     /locus_tag="KRL67_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3787 domain-containing protein"
                     /protein_id="HBH1522787.1"
                     /translation="MESKKARALKGKDNKRLKSSLLVGNESTAAWADVEKLKPHSKVS
                     IPSIKGVEEAKDWVDNGSKL"
     misc_binding    7999..8232
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            8309..9706
                     /gene="asnS"
                     /locus_tag="KRL67_001909"
     CDS             8309..9706
                     /gene="asnS"
                     /locus_tag="KRL67_001909"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HBH1522788.1"
                     /translation="MQKEFITVKELYREKEQYIGKTVKVAGWIRTSRVSKNFGFIELN
                     DGSFFKNMQIVFDEKVENFKEVGKLAISSSILVEGELVSTEGAKQPVEIHAKSIVVEG
                     ESDSSYPLQKKRHTLEYLRTIAHLRPRSNTFSAVFRVRSIAAYAIHKFFQERNFVYAH
                     SPIITGSDCEGAGEMFRITTMDLNDIPKTEDGKIDYTQDFFGKEANLTVSGQLNAEIM
                     ALSFRNVYTFGPTFRAENSYTGRHASEFWMIEPEIVFADLEDNMELAEDMIKYVISYV
                     LENAPEEMEFFNSFIDKGLLERLNKIVNSEFIRITYTKAVELLIESGHEFEYPVEWGC
                     DLQTEHERYITEQIYNCPVFVTDYPKDIKAFYMRMNEDGKTVRAMDLLVPGVGEIVGG
                     SQREEREDVLLDRINEMGLREEDYWWYLELRKFGTATHSGFGLGFERIIMYMTGMSNI
                     RDVIPFPRTPKNAEF"
     gene            complement(9879..11054)
                     /locus_tag="KRL67_001910"
     CDS             complement(9879..11054)
                     /locus_tag="KRL67_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HBH1522789.1"
                     /translation="MNLEKIKKFFGFPLDKKHKKNVEKELCNYLHHTGKIICIIIVIS
                     QIIMMISISAREGGPFATPRRQWYFYMYVTLFCVSSVFMFVFSYLWNKKKDCYTFYIN
                     LWIIYVTFFCLWGCVITLFDQLGGNDLSVFSYIMLAAAAFSVLKFGESVIVFGGCFLF
                     LNLFLPGIQTIPGNEFSNIVNTFFITSLAIFVSTMLYRNRVFTYYDKLLISQQYEEIS
                     HINNLLNDKVMTDDLTKMNNRRYLEDILQKKLISHANQEPITGMMIDIDYFKQFNDNY
                     GHQKGDYCLQNIAKVILDFTSSENEHAIRYGGEEFFICIFNCDEADAKLKSECLRKII
                     ESCNFERDDVPLKCVTVSIGVYTKKIEKTITLNEFISNCDKALYTAKEQGRNCVAFY"
     gene            11473..12837
                     /locus_tag="KRL67_001911"
     CDS             11473..12837
                     /locus_tag="KRL67_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH1522790.1"
                     /translation="MGEKKINNMNYSHNNYSKKTGEPTRKIKNSHITLKSKEEKICSS
                     CNKESNTKDNYCKFCGNELYEITSLRPLETKLDLKSKIKDISYHANKRGVFLTTFTTI
                     FILFIIALIFKAIITIQFNDISYLINPAHIILALNLGQISVSMSTMMGSGFINANIGL
                     LILLIIPILVLLISNLIFMRKRCRDSKTVLANSLGVGIFYGLILAILSIFTNVKTSSH
                     SMLEYGYALEYSYEFFSVLLNGFVLGFICTYITTYKKSYEKENMYLSLFSNSIRTFIL
                     GYVLVLVILLVLTISDSSYLNELDMSSYSNGLNLFSILPQIASYMWAFANGISVTIIN
                     STVSMFTLSSSSLFGDTKLMFYAMGALSMLILLLNGYKLRFKYNIDSIKPIIVFSIYY
                     AFLMGMLALFSTFILDSNINFFNTTSYGATLIMQFKVLQAIVISFVYSFVISLIGYKL
                     NSAD"
     gene            12859..13350
                     /locus_tag="KRL67_001912"
     CDS             12859..13350
                     /locus_tag="KRL67_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HBH1522791.1"
                     /translation="MEGNTNNKVDDKFSKVKKKFGSGKFKVELYKEKSKLNREIDDIQ
                     EKKAKIFLEMGILTYQKIREDSINDNSFDKFCDELLELDKLIYEKNMEINEMEMAESN
                     VTCECGYVGNVNDKYCAECGKKFELENECDDFIVCGYCENEIDTEAEFCPCCGRKIIM
                     DLE"
     gene            13623..14294
                     /locus_tag="KRL67_001913"
     CDS             13623..14294
                     /locus_tag="KRL67_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HBH1522792.1"
                     /translation="MLDKVKGRLIVSCQALENEPLHSPFIMGRMAKAAMEGGAVGIRA
                     QGVEDIIEIKKVTGLPVIGIIKRNYEDSDIYITPTKKEVDELLTTGCEMIALDATNRV
                     RPNNEDLKELIKYIKENGVLVMADISNYDEAIKAQEYGVDCVSTTLSGYTPYTKTLEG
                     PDFVLMERLVKDLEIPVIAEGKVNTPQDLKKVFELGVHSSVVGSAITRPQLITEKFVK
                     AIEIN"
     gene            14379..15245
                     /locus_tag="KRL67_001914"
     CDS             14379..15245
                     /locus_tag="KRL67_001914"
                     /EC_number="4.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003452648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate lyase"
                     /protein_id="HBH1522793.1"
                     /translation="MKGIYSALLVSFDKEGNINEKGLRQIIRHNIDVCKVDGLYVGGS
                     TGENFMLSTDEKKRIFEIAKDEVKEEIKLIAQVGSVNLKEAVELAKFTTDLGYDAISA
                     VTPFYYKFDFEEIKHYYNTIINSVDNRLIIYSIPFLTGVDMSLDQFGELFENEKIIGV
                     KFTAADFYLLERMRKTFPNKLIFAGFDEMMLPATVLGVDGAIGSTFNVNGVRARQIFE
                     LTKNEKISEALEVQHVTNDLITDILGNGLYQTIKLLLEEQGVEAGYCRQPMKEATDEM
                     KSRAKEIYRKYF"
     gene            15278..16780
                     /locus_tag="KRL67_001915"
     CDS             15278..16780
                     /locus_tag="KRL67_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003462875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter"
                     /protein_id="HBH1522794.1"
                     /translation="MVGFTWIDFAILVVYLLAVLFAGLLFSKKEMKGKEFFKGDGTIP
                     WWVTSVSIFATLLSPISFLSLAGNSYGGTWILWFAQLGMFIAIPLTIKFFLPLYSRLE
                     IDTAYEYLEMRYESKGLRVLGALMFIIYQIGRMSIIMYLPSIVLAGLTGIPVNVLIIV
                     MGVIAIIYSYTGGLKAVLWTDFIQGMVLIVGVTGTLFYLIASINGGFGTVMDTLTTGH
                     KFLASNEVMFDKNILSTSAFIIFVGAGLNTFSSYVSSQDIVQRFTTTTDIKQLNKMTY
                     GNGVLSMGLATVFYLIGTCLFIYYTQNPDLAQTVQQDQVFASYIAYELPVGITGILLA
                     AIYAASQSTLSTGLNSVATSWTLDIQTIITKDISMEKQTKIAQYISLGVGILSIAVAI
                     VLANGEIKSAYEWFNSFMGLVLGVLAGIFVLGAFCKKATKMGAYVGFIVSAILVVYLK
                     YRMPEVTSWAYSLITITTSVVVGTIVSAIEAKVKAKVSLPKAETTVYYEK"
     gene            16910..17362
                     /locus_tag="KRL67_001916"
     CDS             16910..17362
                     /locus_tag="KRL67_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HBH1522795.1"
                     /translation="MIFGNINHSKTYSTLHEDLLRCFEYAKDNSLIDYEKGTYTIDGD
                     DIFVNIVEYKTCEKEDRFWEAHKKYIDVHLMLDGCERIDINFIENLEQKIYEEEGDFL
                     PLDGKNNGCVELRKGDFLVCYPEDAHMTGIRVLEKENIKKAIFKVLVR"
     gene            17522..18325
                     /locus_tag="KRL67_001917"
     CDS             17522..18325
                     /locus_tag="KRL67_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH1522796.1"
                     /translation="MENIDVFLLIGQSNARGLGNPKESVIPNENCFEYLSTDEIINMR
                     CELETSEGDGTIAPAFSNEWNKLTGNKVCFIHNAKDGSRIKNWNHDNNWYLNDTIEKF
                     NAGCTTLSKHYNIENKYVIWIQGESDAKYGSDALYYKESLKKIAYRLKEECMIDKMFV
                     SLTGYWLGEDEYFIRTRRIAAAQESACNECDILCVGSKIAMQFHDKGLTIDDVHYTQE
                     GLNILGEDLCKNIYKYHITKEKSVLSDTIDLSEARKYICELEKISKRFV"
     gene            18382..19269
                     /locus_tag="KRL67_001918"
     CDS             18382..19269
                     /locus_tag="KRL67_001918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HBH1522797.1"
                     /translation="MKNYVCIDVGGTSIKYGFIREDGLIIDKSSMDTEAKEKGGEGIL
                     AKIKHIVKKYREENEISGICISTAGMVDPVEGKILFALEELIPNYKGMQLKKEVEKEF
                     NIKCEVENDVNCAGLGEMWLGAGRGATSSICLTIGTGIGGCIIINNKLINGFSNSAGE
                     VGYMNINGSSFQELASTSSLIKKVAKIKNLNENDLNGKIIFDLAKNNDEDCLKELDNM
                     IKSLAVGIANICYVINPEVVILGGGIMAQEKFLKPKIDKALKEVLIERVYKNTNIEFA
                     KRQNDAGMLGALYNFLNKI"
     gene            19288..20136
                     /locus_tag="KRL67_001919"
     CDS             19288..20136
                     /locus_tag="KRL67_001919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HBH1522798.1"
                     /translation="MSILEQLESPTFKVTKSDKLLIAYIKENIDDVFYKPISQIAKES
                     NIGEATITRFVKKMNFNGLQDFKVTLAQEISTINKKNIINKNIQNDEPALDTAKKLLS
                     SNVTTLENTVEIINSKDVHDCARLIINAKKVYFIGIGYSGIIAQDSNYKFMRIGLNCV
                     SFDSSHTMIMMSSIMEEGDLIIAISHSGETEEIIKTVKLAKANNAKIISITENKNSEL
                     KDISDVHLSYVSGETVLETGSISSKLAQFFIIDLVYTQVVKELSNEAIERKIKTTNAI
                     KLFKNE"
     gene            20199..20888
                     /locus_tag="KRL67_001920"
     CDS             20199..20888
                     /locus_tag="KRL67_001920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HBH1522799.1"
                     /translation="MSKLKDYNIDKVIIFNNLSTKTKNQLKEKAKLKRLQKKEILFYE
                     KDSLDSVYILLEGKVSIFKISENGERKVIFILNSGEVINDITFDSSKNSTVGCEVFEN
                     SIVAYYSIEEFLEIMQNDFQLTKDIISYMERRIRRLYRQLKNSISIKVDKKLAAKLYR
                     LSREFGVCCGEWTLLNVNITVTYLADMLGCKRETVSRMIKVLQKEELLKIDNKGFYVK
                     ETELSRYFKNN"
BASE COUNT         8238 a         2230 c         3748 g         6770 t
ORIGIN      
        1 ttattatttt tatatcaaaa atagtaataa agtctatata catttagaaa atactgttta
       61 aaaggagtag aatttatgat aaaaaaagag atatctaact ttagaggaag tagtgattat
      121 gtagtatcac cagaattgat ggcatcagtt aatgttgcta ttgctcttga aaagccactt
      181 ttgattaagg gcgaacctgg aacaggtaaa actatgctag cacaagcaat ttcaaatgaa
      241 ttaaaaaaag atttagtaat ttggaatata aaatcaacta caaaagcaca agaagggctt
      301 tatgtatatg atactgttca aagactttat gatagccaat ttggaggaga aggtgtagat
      361 aatattagca agtacataaa atatggcaaa ctaggagagg cctttagttc aaaccaacaa
      421 gtaattcttt taatagatga aatagataag gcagatttag aatttccaaa tgacttgctt
      481 tgggaattgg ataaaatgga gttttacata aatgagacaa aagaaactgt aagagctaaa
      541 caaagaccaa tagttattat aacttcaaat gcggaaaaag aacttccaga tgctttttta
      601 agaagatgta tatttcatta tatagaattt cctgatagag atatgatgga agaaatagtt
      661 aaagtgcatt ttgataaagt agaagaacac ctattggaac aagtaatgac tactttttat
      721 tggattagaa gtttaaagga catacaaaaa aagcctagta catcagagtt aatagactgg
      781 atacaagcat taactttgag tggaatgcct attgaaaaaa tagaaaaaga agttcctttt
      841 gcaggaatac tgcttaagaa taatgaggat attgaatcta tgcaaagaca cttatagtta
      901 ataggagctg gataattatg tttattgaat ttttttattt attgaggtca agaggattaa
      961 atgtatcttt aaatgaatgg atgactttaa ttgaagcatt ggataaagga ctgtgctatt
     1021 caaacttttc aaatttttac tatctttgta ggatgatttt aataaaaagt gaaagtgatt
     1081 ttgataagtt tgatgcagtg tttttggagt attttaaagg tatagaacac caagaagaaa
     1141 ttccagaaga gattatgaat tggcttaaaa aaccagatat agatttagaa gagtttgaaa
     1201 gattagaaca aaatccaaat ataaatcttg ataaattaag agctaaattg gaggaacgaa
     1261 taaaagaaca agactctcct cataatggtg gtaattattg gataggaaca ggaggcactt
     1321 ccgagttagg acattcagga aaaggtcaaa ctggtatacg tataggtgga aattctactt
     1381 atggtagagc tgttcttgag gttgcaggag aaagaaaata cagagatttt agagatgatg
     1441 aagttttaaa tatgcgtcaa tttcaagttg cactaaagag acttcgtcaa ttttcaacta
     1501 gaatagatgc tccaaagaca gagcttgatt tggataagac gattgaagag acttgtaata
     1561 atgcaggata tttaaagtta ttttttgaga agccaagaaa aaatacagtg aagcttttat
     1621 tgcttatgga ttcaggtggt tcaatgagag ggtatagcac tttatgtaat acattatttc
     1681 agtctgtaag taaatcaaat cattttaaag atgtaaagat atattatttc cataactgta
     1741 tttatgatag attatttaca acacctgaat gttggttaag taaatctatt aatactgaat
     1801 ggatattaaa aaatatagat aaaaactata aagttataat tgttggagat gcttctatgt
     1861 cgccttctga gttacttcat attggtggta actatcgtgg tccatataat tacacacctg
     1921 gtatagagtg gcttaaaagg tttaagagaa agtacagtaa aattgtatgg atgaatccag
     1981 aacttagaga tggatgggat tcaattaatt attggtacca aactcaaaga atgatacaag
     2041 gtgaatttaa tatgtttcca ctgactgtaa aaggtcttga aaaagcattg aaaaatttaa
     2101 tggtttcaag ataaagtaaa tataaatgat ggtatgattt attcaaaatt gtaccatcat
     2161 tatttatttt tctaatatat ctattataaa ttgtataact gaaacttaaa aaattatata
     2221 gtctaataca ttttagactc acacaaaaca tatacattaa taataatttt agaagtcgag
     2281 gtgattataa taaattatga attaattgta aaatacaatg gagatatatt gagattagaa
     2341 gaagaattag gtgtatcagt agagatactt aattcatcat atgcaataat aacatcaagt
     2401 aatgaagaag atgtaaatac acttttaact tatccagaaa ttgaatttat agaaaagcct
     2461 tttatattac agactcaaga tgttcaaagt ttttcaagta caggaataac aggatttaaa
     2521 aatagaactg gtttaactgg aaaaggtact ataattggta taattgattc gggaatagat
     2581 tatactctac ctgtgtttag agatagtgat ggtaggtcta agatattata ttattgggac
     2641 caatctattc aaggaaatcc accagaaggt tttagagaag gaacattata tactaatgaa
     2701 gatataaata atgcaataga tggaagtatg tatataccta tttcaacaac tagcttacat
     2761 gggacacatg ttgcaggtat ttgtgcaact attgcaagtg atgccagaat aatagtggtt
     2821 agagtcggaa atatacagac cgatattttt tcgagaagta cagagtttat gagggcaatt
     2881 aaatttattt tagatagggc attagaatta agaatgcctg ttacattaaa tataagttat
     2941 ggaagtaatg aaggttcaca tagaggaaca tctttatttg aacaatatat agatgatatg
     3001 tgtttatttt ggaaaaacaa tatagttgta gcagctggaa ataatgcaga taaaggaggt
     3061 cataaaagga ttaggcttca aaataatatt acagaagaag ttgagtttat agttggggaa
     3121 ggagagcgta tattaaacat aaatatatgg ccagattttg ttgatgattt tagtgtacat
     3181 ttggtaaacc catcaaataa tcaaacacaa gcaatctctc taacttcagg tgagataagg
     3241 aatactttgg gtgaaactag aataactggt tatttttacc ctatagcacc atattctctt
     3301 acaagaagag ttactctaca attaagttca aatacacaga taactcctgg gttatggaaa
     3361 attgtatttg aaccaataga tatagtaact ggtaatgtaa atatatattt accaacttct
     3421 gaaggactaa atagaaatac tagatttctg attcctactc aagagcttac tgtaacagtt
     3481 cctggaacag caagtagagt tataacagta ggtagtttta attcaagaac agatatagta
     3541 tctatatttt ctggtgaagg agatacacaa ttaggtgtct ttaaacctga tttattagct
     3601 ccaggtgaag atatagtatc ttttttacca ggaggtacaa gtggagcatt aactggaact
     3661 agcatggcta cacctcatgt tacaggggtt tgttcattgt ttatggaatg gggaattgta
     3721 aatggaaatg atttgttttt gtattcacag aaattaaggg cattacttct aaagggagca
     3781 aggagattaa gtaatcaatc ttatccaaat aattcatcag gatttggttt tttaaattta
     3841 tcagatatag atttgtatac tctatctaat ataaatcaag atttagaaac agaggatatt
     3901 ggatatagaa gtattaataa gtcatttaaa gatgaggaaa atagttataa gtttatagat
     3961 ggttataata tgcaaataca taatgactta gaaaatgaaa tttatataag taaaaatgca
     4021 agtagacaat caggaatact atcaggaata gatgtagtcc atactcccga atttgaagaa
     4081 gaactagctg ggttaggtat gggtcaaaat ttttttaaaa taagtgattc attaggtgta
     4141 ctatttataa acaatacaga ctataatagc atacaacgag ttttacaact tccttcaatc
     4201 ataagaactg tatcgactac aaagatgaca ttacttggag aaattaatcg aggtactttt
     4261 ggaggtgtag ttgcaacaga agaaatgggc gtaaatttct ttaaaaataa tcctaatatc
     4321 aatataacag gaagagggac tctaatatca attgcagata caggaataga ttatctacac
     4381 ccagatttta tttatccaga tggcacttca aaaatagtat atttatggga ccaaacaaaa
     4441 gaaggaactc cacctgatgg tttttatatt gggacagaat atacacgaga agatataaat
     4501 cgagctatcg cagaaaatga ccctagtcta tctcaggatg aagttggtca aggaactatg
     4561 ttgagtggta tatgttcagg gcttggaaat gtaaatagtg aatatgcagg tatagcagaa
     4621 gattctgagc taataataat aaaactcggc aagatagatg gtttttataa tagtgcaatg
     4681 ttgtttgctg catctcagta tgcatataaa aaagcttttg aacttggaag accactagtt
     4741 ataaatatgt ctttaggtac cagtagccta gcaggtttaa ccaataggag taatagtgaa
     4801 aaagcattct ttactagagg gttatgtata acggcaggag caggaaatga gggcaataca
     4861 cagacacata catctggaat aataccatat gttggaggtt ctgttgaagt tgagttagaa
     4921 ctaaatgaag aagaggaaga attatcatta gaactttggt taaatagacc agataaagct
     4981 gatgtaataa tagtatctcc aactggcgaa gaaagtaaaa gtgttggagt ttcaaattat
     5041 aataaagtaa caggtttatt tgatttagaa gggactgaat attctataac atatatatac
     5101 ccaacaactt tttctggaca gcaatttact aatgttactt taaagaatgc aaaaagaggt
     5161 gtatggaaga ttcgattagt aggagtttat attataacag gaagatataa cttatacctt
     5221 ccaaatagag aacttttaaa aagtggaact agatttagag aggtagaccc tttttacact
     5281 ataaattatc ctgcgattca agatgattta ataactatag gagcctacaa tactattaat
     5341 ggtagtttat ggcaaagttc atcaagagga ccaactattg aagacaggtt aaaaccagat
     5401 atagtagcac caggtgttaa tataatagct gcttatcctg ggaacacgta tgcaactata
     5461 actggtacag cagcagcaag tgcacatgca gctggtgctg ctgcaatgta ttttcaatat
     5521 acttttgttg atggtagata cccaaaccaa gcatatgtgc aaaaaataaa aacttttatg
     5581 caagctggtg ctagaaaaga ttcaaacaca gtatatccaa atacaaattc tggatatggg
     5641 cttttggacg ttagaggaat gtttgatgtt ttaagatagg agggatttat ggaaaaatct
     5701 tattgtataa tttatcaagg taatatagaa tctgccctac aagaaaatgg aataaataag
     5761 tatatggttt taaatagtca gcttgcagta atatatgtac ctattgattt tgatgaaact
     5821 attttaaata atataataca agttgcttgg tgggaagaat ctgaaccaat gagcagtcta
     5881 attgaaataa ctaataatgt aaacaatgga gaaaccatta caacagcagc tgaaactgat
     5941 tatatttatg aaaatccata taatgatata acgggaagag gaattttatt agcagttata
     6001 gattctggga tagattattt gcatcctgat tttataaatg atgatggaag tagtaaagtt
     6061 ttatatttat gggaccaaga agcaaataca aatccacctc cagaaggttt tatttttgga
     6121 agtgaattta caagaagtga gttaaatata gccataaata gaaatgatgg aagtttgagt
     6181 caagataata taggtactgg aactttagtt tctggtatat tagctggaaa tggaagggta
     6241 aattctcaat acagaggtat tactacagaa tcagacttaa tagtagttaa attaaaatca
     6301 tatacagata cttattatgc tggaaggata aattacagtg tttcagattt tttagcagct
     6361 ataacgtatg ttactaatat agcaagaaca gaaaataaac cattaataat aaatttgact
     6421 attggagtta aatcaggagc tgtagcaact acttcaatac tagatacttt taatatattg
     6481 agtagtgctg gtgtggtagt agtgagtggt gcaggaaatc aagggaatac agatattcat
     6541 tattctggca gatttaacag tgtaggagaa gttcaagatg taataataca agacggagat
     6601 gactatgcac ttgatataac gctaaatacg aatggtccag ataaaatagg agcacaaata
     6661 atatctcctt caggtgaagt tagtcatgat ataaggtatt ctccagactt ttatatatat
     6721 agaggaaaat ttaacttaga aaatactact tatgcaatga gatttattta tccatatatt
     6781 acttctggaa aagaaaatct ggagataaga ttaagagata taaaacctgg agtatggatt
     6841 ttaagattga cttcagaact tataataggt ggagaatatg acatatactt gccaaataaa
     6901 aatttgatag ctccagatac aagattttta gaccctgatt cagtagctac aataactatg
     6961 tatgctgcta gtgatgatgt aattacagtt ggtacattta ataataagac tgatagtatg
     7021 tggattggtt cttcgaaagg accaattaga ggaagaggaa ttaagccaga tatagtagct
     7081 tcaggtgtag atataatatc cacatataaa aatggagcat ataatacagg aacaggaaca
     7141 ggagtgagta gttctatagt cacaggtgtt ttagccttgt tgatggaata cttagaaaaa
     7201 caggataatg taccgagatt atcattgttt acacaggttt taaaaacata tttaatattg
     7261 ggtgctacaa aacttgagat atatacatat ccaaatgtat cgcagggata tgggatttta
     7321 aatttaaaaa atacaattca acagatagca aatactctat agataagaac ctatgtaaag
     7381 ttataattga ttaatagtta aattttatta tgtagtctag atagggtgga aaaatagcat
     7441 aaaaaataaa tatgaaaatt aaaaaaacag ttttatattt tgaatacaca aagtaaatat
     7501 tttatatata aataggtata aattaagtat tgaaaagata tgatgttaat ttaaaaatca
     7561 ttataattta gacttatgaa ttttatttgt aaaattgcta aaactattac aaaataaata
     7621 atagaggtga tagttttgga aagtaaaaag gcaagagcat taaaaggtaa agataataaa
     7681 agacttaaaa gttcactttt agttggaaat gagtcgacag cagcatgggc tgatgttgaa
     7741 aaattaaaac ctcatagtaa agtttcaata ccttcaatta agggtgtcga agaagcaaaa
     7801 gattgggttg acaatggaag taaattataa gattttggaa attgaagcaa taaagaagta
     7861 ataaagataa gtatttatta tcattatgca agttagattg attatatttt aaataacaat
     7921 cagtatcaat tatataaatt tattgactaa aaatttataa attggtataa ttttgttaat
     7981 tagcagaaat aataaaagat aaatactgtg aagagaagag tagatataaa atgtagttct
     8041 agcgagttga ggatggtgaa agctcaatac gaggtttata ttgaagaacg tcttggagtt
     8101 tctaatcgaa agcttattta gtaagtagac ttagacggaa ggcaccgtta taggctttaa
     8161 gcattgctta actaagaggt cattatgaca actagggtgg taccgcgaga gtaatagtct
     8221 ttcgtccctt tatataggga tgaagggctt tttttattga aattatattt aaaagaaatc
     8281 aataacaatt atactaggag gatttaaaat gcaaaaggaa tttataacag taaaagaatt
     8341 gtacagagaa aaagaacagt acatagggaa gactgtaaaa gtagctggat ggataagaac
     8401 atctagagta tctaaaaatt ttggatttat agagctaaat gatggaagct tctttaaaaa
     8461 tatgcagata gtatttgatg agaaagtaga aaattttaaa gaggtaggaa aattagcaat
     8521 aagctcatct atacttgtag agggagagtt agtatctaca gaaggagcta aacaaccagt
     8581 cgaaatacat gcaaaaagta tagttgtaga aggggaatca gatagttcgt atccactaca
     8641 aaagaagaga catacgcttg aatatttaag aacgatagca catttaagac caagaagtaa
     8701 tactttttca gctgtattta gagtaagatc aatagctgcc tatgcaatac acaaattttt
     8761 ccaagaaaga aactttgtat atgcacattc tccaatcata acaggtagtg actgtgaagg
     8821 tgcaggagaa atgtttagaa taacaactat ggatttaaat gatattccaa aaactgaaga
     8881 tggcaaaata gactatactc aagatttctt tggaaaagag gctaatttaa cagttagtgg
     8941 tcaattaaat gccgagataa tggcactttc ttttagaaac gtttatacat ttggacctac
     9001 atttagagct gaaaactctt atacaggaag acatgcttca gaattttgga tgatagagcc
     9061 agaaatagtt tttgcagacc ttgaagataa catggaactt gcagaagata tgataaaata
     9121 tgttataagt tatgtattag aaaatgctcc tgaagaaatg gaatttttta atagttttat
     9181 agataaagga ttgttagaaa gattaaataa gatagtaaac tctgagttta ttagaataac
     9241 gtatactaaa gcagtggaat tattaattga atctggtcat gaatttgagt atcctgttga
     9301 gtggggatgt gaccttcaaa ctgagcatga aagatatata acggagcaaa tatataattg
     9361 tccagtgttt gttacagatt atccaaaaga tataaaagct ttctatatga gaatgaatga
     9421 agacggtaaa actgttagag caatggattt actagttcct ggagttggag aaatcgtagg
     9481 aggttctcaa agagaagaaa gagaagatgt attgttagat agaattaatg aaatgggatt
     9541 aagagaagaa gattattggt ggtatttaga acttagaaag tttggtactg ctactcactc
     9601 tggatttggt cttggatttg aaagaataat tatgtatatg acaggtatgt ctaacataag
     9661 ggacgttata cctttcccaa gaactcctaa aaatgctgaa ttttaataat tatatataat
     9721 aaaacttata tagctttaag tataaacact aaaatgactt gcatatttat ctattttaga
     9781 gatatgcaag ttatttttat tgtactataa gtatataatt tgattacttt tattttgcga
     9841 tataatactt ataggtttat gtaatagtat gtaacttttt aataaaatgc aacacaatta
     9901 cgtccctgtt cttttgcagt gtatagagcc ttatcacaat tacttataaa ctcatttaat
     9961 gttattgtct tttcaatttt ctttgtataa actccaatac taacagttac acattttaaa
    10021 ggaacatcat cacgctcaaa attacaggac tctataattt ttcgtaaaca ttctgattta
    10081 agtttagcat ctgcctcatc acagttaaaa atacatataa aaaattcctc tccaccatat
    10141 cgtatagcat gttcattttc agagcttgta aaatctaaaa ttactttggc aatattttgt
    10201 agacaatagt ctcccttttg atgtccataa ttatcattaa attgtttgaa ataatctata
    10261 tctatcatca ttccagttat aggttcttga tttgcatggc tgattaattt tttttgtaga
    10321 atatcttcaa ggtatctacg gttgttcatt ttagtaaggt catctgtcat aactttgtcg
    10381 ttaagtaaat tatttatatg agatatttct tcatattgtt gactaattaa aagtttatca
    10441 tagtaagtaa atacacgatt tctatatagc attgtagaaa caaaaatggc aagggatgtt
    10501 ataaaaaatg tgttgacaat attactaaac tcattgcctg gtatagtttg aatcccaggt
    10561 aagaataaat taagaaacaa aaagcatcct ccaaaaacta ttactgattc tccaaatttt
    10621 aatactgaga aagctgcagc tgctaacata atgtaactaa atacacttag gtcatttcca
    10681 cctaactggt caaatagagt aattacacaa ccccatagac agaaaaaagt aacataaatt
    10741 atccataaat ttatataaaa cgtataacaa tccttttttt tattccaaag ataagaaaat
    10801 acaaacataa agacagaaga tacacaaaat agtgttacat acatataaaa ataccattgg
    10861 cgtcttggtg tggcaaaagg gcctccttct ctagctgaga ttgatatcat cataattatt
    10921 tgagatatta ctattattat gcaaatgatt ttacccgtat gatgaagata attgcagagt
    10981 tctttctcaa catttttttt atgttttttg tctagaggaa atccaaaaaa tttttttatt
    11041 ttttctaagt tcatataagc ctccattaaa aataaagcat aaaaataagt attcctttct
    11101 gtccttagat acttggaata cttcgtttat gatttaatta tatcaaaata tattgtgtgc
    11161 aacaatctaa aattgtgata gataaaattt attagctaat gtcgagtaac tttattgatt
    11221 aatactttat tataattaag atttacagat agtttgctac tgaaaataaa gaatagaata
    11281 aatttaaatt aagcataata aaatataaat aataaagttc gcattaacaa actatttaaa
    11341 tatcaagacc tgttaaaaaa caacaccaat aaacaaataa aatagataaa tatatccaat
    11401 taaggtataa ttaagtttta tatgatatag ttatatttga taatttacaa tagtaaaact
    11461 tggagggaaa aaatgggtga aaagaaaata aataatatga attattcaca taataattat
    11521 tccaaaaaga caggtgaacc aactagaaaa ataaaaaata gtcatatcac tcttaaaagt
    11581 aaagaagaaa aaatttgttc tagttgcaat aaagaaagta atactaagga taattactgt
    11641 aaattttgtg gaaatgaact ttatgaaata acatcattaa gaccacttga aacaaaacta
    11701 gacttgaaat ctaaaataaa agatatttca tatcatgcaa ataaaagagg tgtattttta
    11761 acaacattta caactatatt tatactattt attattgcat tgatatttaa agctattata
    11821 actattcaat ttaatgatat tagttattta ataaatccag cacatataat cctagctctc
    11881 aacttgggtc aaattagtgt atctatgtct acaatgatgg gttctgggtt tataaatgcc
    11941 aatataggtt tgttgatatt acttataatc cctattttgg tattattaat atcaaaccta
    12001 atttttatga gaaaaagatg tagagattca aaaactgtat tagcaaattc tctaggagtt
    12061 ggaatttttt atggattaat tttagctatt ttatctattt ttactaatgt aaaaactagt
    12121 tcacatagta tgttagaata tggatatgca ttagaatata gttatgaatt ttttagtgtt
    12181 cttttaaatg gatttgtatt agggtttata tgtacataca taactactta taaaaaaagt
    12241 tatgaaaagg aaaatatgta tttatctctt ttttcaaatt ctataagaac ctttatatta
    12301 ggttatgttt tagtattagt aatactattg gtactaacta tatctgatag tagttactta
    12361 aatgaactag atatgagtag ttattcaaat ggattaaatt tatttagtat actgccacaa
    12421 atagctagct atatgtgggc ttttgcaaat ggaatttcag taaccataat aaattcaaca
    12481 gtatcaatgt ttactttaag ttcttcatct ttatttggag atactaaatt aatgttttat
    12541 gcaatgggag cattgtctat gttaatatta ttattaaatg gatacaagct aagatttaag
    12601 tataatatag atagtataaa acctataata gtatttagta tatattatgc atttttgatg
    12661 ggaatgttgg ctttatttag cacatttata ctagatagta atattaattt ctttaataca
    12721 actagttatg gagcaacttt aataatgcaa tttaaagtct tacaagctat tgtaatttca
    12781 tttgtatata gttttgtgat atctttaata ggttataagc taaactcagc tgattaaata
    12841 tgattggagg aaacttctgt ggaaggaaat acaaataata aagtagatga taaattttct
    12901 aaggttaaga aaaagtttgg tagtggaaaa tttaaagttg aattatataa agaaaaatca
    12961 aaattaaata gagagattga tgatattcaa gaaaagaaag caaagatatt cttggaaatg
    13021 ggaatactga catatcaaaa aattagagaa gattctatta atgataatag ttttgataaa
    13081 ttctgtgatg agttactaga acttgataag ttaatttatg aaaagaatat ggaaattaat
    13141 gaaatggaaa tggcagaaag caatgtaact tgtgagtgtg gttatgttgg aaatgtaaat
    13201 gataaatatt gtgctgaatg tggtaaaaaa tttgaattgg aaaatgaatg tgatgatttt
    13261 atagtttgtg gttattgtga aaatgagata gatacagagg ctgaattttg tccatgctgt
    13321 ggaagaaaaa ttataatgga tttagaataa tattgggaaa cctatgtata gtatacatgg
    13381 gtttttttat tgaaattatt taaatggact tataacagtg gttatataaa atgaaaataa
    13441 acaccaaaaa aatgaaataa attttctttt aagcctaaaa aacaagctaa tatatagaat
    13501 aaatcagtaa aaatatagag gtgagaaaaa aatatgaaaa aatattgcaa agaaaaagtt
    13561 ttcatgttat tattaataca taaatcaaga aaataaattt cagaaacgag gtaatttaaa
    13621 aaatgctaga taaggtaaag ggaagattaa tagtatcatg ccaagcttta gaaaatgaac
    13681 ctcttcatag tccttttata atgggtagaa tggctaaggc tgctatggaa ggtggagctg
    13741 ttggaattag agctcaagga gttgaagata ttattgagat aaaaaaagtt actggacttc
    13801 ctgttatagg tattataaaa agaaattatg aagattcaga tatatatata acgccaacta
    13861 aaaaagaagt tgatgaactt ttaacaacag ggtgtgaaat gatagcttta gatgctacta
    13921 atagagttag accaaataat gaagacttaa aagaacttat aaagtatata aaagaaaacg
    13981 gtgttctagt aatggcagat atttcaaatt atgatgaggc tatcaaagca caagagtacg
    14041 gtgtagattg cgtttcaact acactatctg gatatactcc atatactaaa acattagaag
    14101 gcccagattt tgtattaatg gaaagattag ttaaagattt agaaatacct gtaatagcag
    14161 aaggaaaggt aaatactcct caagatttaa aaaaggtttt tgaactagga gttcattcat
    14221 cagtcgtagg ttcagcaata acaagaccac aattaataac agaaaaattt gttaaagcaa
    14281 ttgaaattaa ttaaaaatta atttataaat taaaaaatta ttatatttaa ctatacttga
    14341 aaattaaaat tggaggtaaa gatatgagaa caacagatat gaaaggaatt tattcagcat
    14401 tattagtttc atttgataag gaaggaaata taaatgaaaa agggttaaga caaataatta
    14461 gacataatat agatgtttgt aaagtagatg gtctttatgt aggaggaagt acaggggaaa
    14521 acttcatgct ttcaactgat gaaaagaaaa gaatatttga aatagctaaa gatgaagtga
    14581 aagaagaaat aaaattaata gctcaagttg gttcggttaa tttaaaagaa gctgtagagc
    14641 ttgctaaatt tacaactgat ttaggatatg atgcaataag tgctgttaca cctttttact
    14701 ataaatttga ttttgaagaa ataaaacact attataatac aataataaat tctgtagata
    14761 atagattaat aatatactct ataccatttt taactggtgt tgatatgagt ttagaccaat
    14821 ttggagaatt atttgaaaat gaaaaaataa taggtgttaa gtttacagca gcagatttct
    14881 acttattaga gagaatgaga aaaacattcc caaataaatt aatatttgca ggatttgatg
    14941 aaatgatgtt accagctaca gttttaggtg tagatggggc aattggttct acattcaatg
    15001 taaacggtgt cagagctaga caaatatttg aattaactaa gaatgaaaaa atatcagaag
    15061 cacttgaagt acaacatgtg actaacgatt taataacaga tatattagga aatggtttat
    15121 atcaaacaat taaattacta cttgaagaac aaggtgttga ggctggatac tgtagacaac
    15181 ctatgaaaga agcaactgat gaaatgaaat caagagcaaa agaaatatac agaaagtatt
    15241 tttaataaaa tacttttagt atgggaggat tagtcaaatg gtaggattta cttggataga
    15301 ttttgcaata ctagtggttt acttattggc agttttattt gcaggattgt tattttcaaa
    15361 aaaggaaatg aagggaaaag agttcttcaa aggtgatgga actattccgt ggtgggttac
    15421 atcagtatca atatttgcca cattattaag tccaatatcg tttttatcat tagcaggaaa
    15481 ttcttatggt gggacttgga ttttatggtt tgcacaatta ggtatgttta tagctatacc
    15541 tttaacaata aaatttttct tacctctata tagtaggtta gaaatagata cagcttatga
    15601 gtacttagaa atgagatacg aaagcaaagg tcttagagtg cttggagctt taatgtttat
    15661 aatatatcaa ataggacgta tgtctattat aatgtatctt ccatcaatag ttctggctgg
    15721 tcttacggga atacctgtaa atgtactgat aatagttatg ggtgtaatag caataattta
    15781 ctcatataca ggtggattaa aagctgtctt gtggaccgat tttatacaag ggatggtatt
    15841 aatagtaggt gttacaggga cattgttcta tttaatagca agtataaatg gtggttttgg
    15901 aacagtaatg gatactctta caactggaca caagttctta gcaagtaatg aagtcatgtt
    15961 tgataaaaat atactgtcta caagtgcatt tataatattt gttggagctg gtcttaatac
    16021 tttttcatca tatgtatcga gtcaagatat agttcaaagg tttactacaa caactgacat
    16081 aaagcaactt aataaaatga catatggaaa tggcgtttta tcaatgggac ttgcaacagt
    16141 attttatcta attggaactt gtttatttat atactatact caaaacccag atttagctca
    16201 aacagttcaa caagaccaag tatttgcatc atatatagct tatgaattac cagtaggtat
    16261 aacaggaata ctacttgcag cgatatatgc agcttctcaa tcaactttat cgacagggct
    16321 taactcagtt gctacaagtt ggactttaga tatacaaact atcataacaa aagatataag
    16381 tatggaaaaa caaactaaga tagcacaata tatatcatta ggtgtaggta tactttctat
    16441 agctgttgca atagtattag caaatggaga aataaagtca gcatatgaat ggttcaatag
    16501 ttttatgggg ctagtactag gtgttttagc aggaatattt gtacttggtg cattttgtaa
    16561 aaaagcaact aaaatgggag catatgttgg attcatagta tcagcaattt tagttgttta
    16621 tttaaaatac cgtatgccag aagtaacttc ttgggcatat tctctaataa caatcacgac
    16681 ttctgtagtg gtaggtacaa tagttagtgc aatagaagct aaagttaaag caaaagtatc
    16741 tttaccaaaa gctgagacta ctgtttatta tgaaaaataa tatttaaatg ataaattgtc
    16801 ttatagagtg attagataaa taatttagtt actctataag attgtataat atactttata
    16861 tataaaagta taaaaataga ttttataatt atatagggag atagtgctta tgatatttgg
    16921 aaatataaat catagtaaga catattcaac attgcatgaa gatttactta gatgttttga
    16981 atatgcgaaa gataattcat taatagatta tgaaaaaggt acttatacaa tagatggtga
    17041 tgatattttt gtgaatattg tagagtataa aacttgtgaa aaagaagata gattctggga
    17101 agcgcataag aagtatatag atgttcattt gatgcttgat ggatgtgaac gaatagatat
    17161 aaattttata gaaaatttag aacaaaaaat atatgaagaa gagggagatt tcttacctct
    17221 tgatggtaaa aataatggat gcgtggaact tagaaaaggt gattttttag tctgttatcc
    17281 agaagatgct catatgacag gaataagagt tttagaaaaa gaaaatatca aaaaagctat
    17341 ttttaaggtt ttggttagat aatgataatt tatataacca ctagggctga aaatagtgat
    17401 ttaactttca gcgattagtg agacttcgta aattttagtt agattactat tttcagcctt
    17461 agttttttat gagaaaattg aaaataatta aaagcttaaa attttgtaaa ggagttaact
    17521 tatggaaaat atagatgtat tcttattaat aggacaaagt aatgctagag gactaggaaa
    17581 tccaaaagag agcgtcattc caaatgaaaa ttgctttgaa tatttgagta ctgatgaaat
    17641 tataaatatg agatgtgagt tagagacctc agaaggtgat ggtactatag cacctgcatt
    17701 ttcaaatgaa tggaataagc tcactggaaa taaagtttgt tttattcaca atgcaaaaga
    17761 tggttcaaga ataaaaaatt ggaatcatga taataactgg tatctaaatg acactataga
    17821 aaaatttaat gcaggctgta ctaccttaag taaacattat aatatagaaa ataaatacgt
    17881 tatttggatt caaggtgaaa gtgatgcaaa atatggtagt gatgcattgt actataaaga
    17941 aagtctaaaa aaaattgcgt ataggcttaa agaagaatgt atgattgata aaatgtttgt
    18001 aagtttaact ggttattggc ttggagaaga tgaatatttt attagaacta gacgaattgc
    18061 agcagctcaa gaatctgctt gtaatgaatg tgatatactg tgtgtaggaa gtaagattgc
    18121 tatgcaattt catgacaaag gcttaactat tgacgatgtt cattatactc aagaaggtct
    18181 taatatttta ggagaggact tatgtaaaaa tatttataag tatcatataa ccaaagaaaa
    18241 aagtgtatta agtgatacta tagacttatc agaagcaagg aaatatatct gtgaattgga
    18301 aaagataagt aaaagatttg tgtaatataa attttaaata gagttgttaa tttataaaaa
    18361 taatttgtat ggagatatgt tatgaaaaat tacgtttgta tagatgttgg aggtacttca
    18421 ataaaatatg gttttattag agaagatgga ttaataattg ataaatccag tatggatact
    18481 gaagcaaagg aaaaaggtgg agaaggaata ttagccaaaa taaaacatat agtaaaaaaa
    18541 tatagagagg aaaatgaaat aagtggtata tgcatatcaa ctgcaggtat ggttgaccca
    18601 gttgaaggta aaatactatt tgcattagaa gaattaattc caaactataa gggtatgcaa
    18661 ttaaaaaaag aagtagaaaa agagtttaat ataaaatgtg aagttgaaaa tgatgttaat
    18721 tgtgctggac ttggtgaaat gtggcttgga gcaggtagag gtgcaacatc ttctatatgt
    18781 ttaacaatag gtactggaat tggtggatgt attataataa acaataaact gataaatggg
    18841 tttagtaata gtgctggtga agtaggatat atgaatataa atggaagttc ttttcaagaa
    18901 ttggcatcta ctagcagcct aattaaaaaa gtagcaaaaa taaaaaattt gaatgaaaat
    18961 gacttaaatg gaaaaataat ttttgattta gcaaagaata atgatgaaga ttgtttaaaa
    19021 gaactcgata atatgataaa atcattagca gttggaatcg caaatatatg ttatgtaata
    19081 aatccagaag tggtaatctt aggtggtggg attatggcac aagaaaagtt cctaaaacca
    19141 aaaatagaca aagcattaaa ggaggttctt atagagagag tttacaaaaa cactaatata
    19201 gaatttgcaa agagacaaaa tgatgcagga atgcttggag cattatataa ttttttaaac
    19261 aaaatataaa gaatagaagg gtttttaatg agtattttag aacaattgga atcaccaact
    19321 tttaaagtca caaaatcaga taaattatta atagcttata taaaagaaaa tatagatgat
    19381 gtattttata aaccaatatc acaaatagct aaagagagca atattggtga agcaactata
    19441 actaggtttg taaaaaaaat gaattttaat ggattacagg attttaaagt tactttagca
    19501 caagaaatat caactatcaa taaaaaaaat attataaata aaaatataca aaatgatgag
    19561 ccagctttgg atacagcaaa aaaactttta agttcaaatg taaccacttt agagaacaca
    19621 gtagagataa tcaatagtaa ggatgtacat gactgtgcaa ggcttataat aaatgcaaag
    19681 aaagtttact tcataggtat tggatattct ggtataatag cacaggattc aaactataaa
    19741 tttatgagaa taggtcttaa ttgtgtaagt tttgacagta gccatacgat gataatgatg
    19801 tcttccataa tggaagaagg agatttaata attgcaattt cacattctgg tgaaacagag
    19861 gagattataa aaactgtaaa gcttgcaaaa gccaataatg ctaaaattat atcaattact
    19921 gaaaataaaa attctgaatt aaaggatatt tcagatgtac acttatctta tgtatcagga
    19981 gaaacagtac tggaaacagg ttcaatatct tctaaattag cacaattttt cattattgat
    20041 ttagtatata cacaggtagt aaaagaactt tctaatgaag ctattgaaag gaaaattaaa
    20101 actacaaatg ctataaaatt gtttaaaaat gaataaaata agaaatttgt aatgttttat
    20161 agagctctct taaaaattta ggaatggaga gatattacat gtctaaatta aaagattata
    20221 atattgataa agtaataatt tttaataatt taagtactaa aacgaagaat caacttaaag
    20281 aaaaagctaa actaaaaaga ttacaaaaaa aagaaatttt attttatgaa aaagatagct
    20341 tagacagtgt ttatatactt ttagaaggaa aggtatctat ttttaagata agtgaaaatg
    20401 gagagagaaa agtaattttc atattaaata gtggggaagt cataaatgat attacatttg
    20461 actcttctaa aaactctact gttggctgtg aagtatttga aaattcaatt gtagcttatt
    20521 atagtattga agaattttta gaaattatgc aaaatgattt tcaacttaca aaagatatta
    20581 tttcttatat ggaaagaaga ataagacgtt tatatagaca gttgaaaaat tctatatcca
    20641 taaaagtaga taaaaaactt gcagcaaaat tatataggtt aagtagagaa tttggagttt
    20701 gttgtggaga atggacttta ctaaatgtaa atattacagt tacatattta gctgatatgc
    20761 ttggctgtaa aagggagact gtttctagaa tgataaaagt attgcaaaaa gaagaacttc
    20821 ttaagattga taataaaggt ttttatgtta aagaaactga attatcaagg tattttaaaa
    20881 ataattaatt atgattaagt aagagtgttg ttagaatttt aattaaataa aattattatt
    20941 aggagaatct atttatagat tcttttttat tgtctaattt attaat
//
LOCUS       DAEPXF010000063        20769 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain HB27
            SAMN13376389-rid12479423.denovo.067, whole genome shotgun sequence.
ACCESSION   DAEPXF010000063 DAEPXF010000000
VERSION     DAEPXF010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13376389
            Sequence Read Archive: SRR10518432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 20769)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20769)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001069624.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001069624.1
            Genome Coverage       :: 315x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/15/2021 01:54:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,165
            CDSs (total)                      :: 4,076
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 89
            rRNAs                             :: 10, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 10 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 19 of 69
            Pseudo Genes (incomplete)         :: 44 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 10 of 69
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20769
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13376389-rid12479423.denovo.067"
                     /strain="HB27"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="China: Hebei"
                     /collection_date="2017"
     gene            356..667
                     /gene="rpsJ"
                     /locus_tag="KRL67_001921"
     CDS             356..667
                     /gene="rpsJ"
                     /locus_tag="KRL67_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HBH1522800.1"
                     /translation="MAKNEKIRIRLKSYDHKLLDFSAGKIVETAKKAGSQVSGPVPLP
                     TEKQVVTILRAVHKYKDSREQFEIRTHKRLIDIANPTPKTVDSLMRLDLPAGVDIEIK
                     L"
     gene            759..1388
                     /gene="rplC"
                     /locus_tag="KRL67_001922"
     CDS             759..1388
                     /gene="rplC"
                     /locus_tag="KRL67_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HBH1522801.1"
                     /translation="MKGILGKKVGMTQIFTDKGVVIPVTAVEAGPMVVTQIKTVDKDG
                     YNAIQIGFEDAKEKALNKPKKGHLAAANVLKKHLKEFRVDSVEGYTVGQEIKADVFEA
                     GAKIDVTGISKGKGFQGPIKRHGQSRGPETHGSRYHRRPGSMGACSYPGRVFKNKKLA
                     GHMGSVKVTVQNLEVVKVDADKNLILVKGAIPGAKGSVVTIKEAIKVSK"
     gene            1418..2041
                     /gene="rplD"
                     /locus_tag="KRL67_001923"
     CDS             1418..2041
                     /gene="rplD"
                     /locus_tag="KRL67_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001127258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HBH1522802.1"
                     /translation="MPKLNVLNVSGQNVGEIELSDSIFGVEVNGHVLYEVVKNQLANK
                     RQGTQSAKTRAEVRGGGRKPWKQKGTGRARQGSTRSVQWVGGGVAFAPKPRSYKYTLP
                     KKVRRLAMKSALSSKVQNSEVIVLDALNMDAPKTKEFAQILNNINAAKKALVVIADKN
                     DNVIKSARNIEGVQTALVNTMNVYDILKYDSFIITTDAVKKVEEVYA"
     gene            2041..2331
                     /gene="rplW"
                     /locus_tag="KRL67_001924"
     CDS             2041..2331
                     /gene="rplW"
                     /locus_tag="KRL67_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001205558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HBH1522803.1"
                     /translation="MTNPHDVIIRPVVTEHSMAEMGEKKYTFVVAKDANKTEIKKAVE
                     KVFGVSVDKVNTLNYDGKVKRMGRTQGRTASFKKAVVKLTADSKEIEFFQGM"
     gene            2361..3191
                     /gene="rplB"
                     /locus_tag="KRL67_001925"
     CDS             2361..3191
                     /gene="rplB"
                     /locus_tag="KRL67_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HBH1522804.1"
                     /translation="MAIKKFRPTSPALRQMTVLVSDEITCNQPEKSLLVNLKKNAGRN
                     VHGRITVRHRGGGQKRKYRIIDFKRDKDGIPAKVATIEYDPNRTANIALLNYADGEKR
                     YILAPVGINVGDTILSGLGADIKPGNCLALKDMPVGTIIHNIELKPGKGAQLVRSAGV
                     SAQLMAKEGKNALLRLPSGEMRLVSINCKATIGQVGNIEHGNVVIGKAGRKRHMGIRP
                     TVRGSVMNPNDHPHGGGEGRSPIGRPSPVTPWGKPALGYKTRKKNKASNKLIVSRRTK
                     "
     gene            3226..3507
                     /gene="rpsS"
                     /locus_tag="KRL67_001926"
     CDS             3226..3507
                     /gene="rpsS"
                     /locus_tag="KRL67_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003454422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HBH1522805.1"
                     /translation="MSRSTKKGPFVHARLLKKIEAMNASGNKEVIKTWSRSSTVFPQM
                     VENTIAVHDGRKHVPVYITEDMVGHKLGEFVPTRTFKGHKDDEKSNKRK"
     gene            3539..3874
                     /gene="rplV"
                     /locus_tag="KRL67_001927"
     CDS             3539..3874
                     /gene="rplV"
                     /locus_tag="KRL67_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018590372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HBH1522806.1"
                     /translation="MEAKATAKYVRVSPRKAGQICDLVRGKNVDEALAILKFTPRGAA
                     SIIAKVVKSAKANAENNHEMDTEKLYIASIVANQGPTMKRFMPRAMGRATTIRKRTSH
                     IEVVVKEKK"
     gene            3897..4712
                     /gene="rpsC"
                     /locus_tag="KRL67_001928"
     CDS             3897..4712
                     /gene="rpsC"
                     /locus_tag="KRL67_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HBH1522807.1"
                     /translation="MGQKVNPHGLRVGVIKDWDSRWFATDKKEFGNLLLEDHNIRKFL
                     KKRLYSAGVAKIEIERSANKIKMDLHVAKPGVVIGRAGAGIEALKAELEKMTKKTIIV
                     NIVEVRSTDKNAQLVAENIALAIERRVAFRRAMKQAIQRAMKSGAKGIKVSASGRLGG
                     AEMARTEGYSEGNVPLQTLRADIDYGFAEADTTYGKIGIKVWICNGEVLPTRDGVNPR
                     EESRKSDRRDNKRDNRRNDRRGNDRRGNDNRGNYRGQRPQGGSRPQRTENKGN"
     gene            4748..5179
                     /gene="rplP"
                     /locus_tag="KRL67_001929"
     CDS             4748..5179
                     /gene="rplP"
                     /locus_tag="KRL67_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HBH1522808.1"
                     /translation="MLMPKRVKRRRVHRGSMAGQAHKGNKVTYGEFGLVALEASWITS
                     NQIEAARIAMTRYIKRGGKVWIKIFPHKPVTRKPAETRMGAGKGSPEYWVAVVKPGRV
                     MFELAGVSEDKAREAMRLAAHKLPIKCKFVKKEDLEVKGGE"
     gene            5181..5384
                     /gene="rpmC"
                     /locus_tag="KRL67_001930"
     CDS             5181..5384
                     /gene="rpmC"
                     /locus_tag="KRL67_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005810148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HBH1522809.1"
                     /translation="MKAKELRDLTSEELMNKLNDFKSELFSLRFQLATGQLENTARIK
                     FVKKDIAKVKTVLAERKLYETRA"
     gene            5408..5662
                     /gene="rpsQ"
                     /locus_tag="KRL67_001931"
     CDS             5408..5662
                     /gene="rpsQ"
                     /locus_tag="KRL67_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018590376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HBH1522810.1"
                     /translation="MERGRRKVRIGRVVSDKMDKTIVVAVEEFVRHPLYNKRVKRTKK
                     FKAHDEKNICNIGDRVKIMETRPLSKDKRFRLVEVVEKVK"
     gene            5688..6056
                     /gene="rplN"
                     /locus_tag="KRL67_001932"
     CDS             5688..6056
                     /gene="rplN"
                     /locus_tag="KRL67_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007885948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HBH1522811.1"
                     /translation="MIQQESRLRVADNSGAKELLCIRVLGGSKRRYGNIGDVIVATVK
                     SATPGGVVKKGKVVKAVIVRSKQGVRRNDGSYISFDENAAVIIKDDKTPVGTRIFGPV
                     ARELRDNEFMKIVSLAPEVL"
     gene            6080..6385
                     /gene="rplX"
                     /locus_tag="KRL67_001933"
     CDS             6080..6385
                     /gene="rplX"
                     /locus_tag="KRL67_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HBH1522812.1"
                     /translation="MRVKKGDTVVVIAGKDKGKKGSVLKVYPKTSKVLVEGVNVITKH
                     QKPSAMNQQGGIINKEAPIHISNVMPFDPETGKGVRVRYEVKDGNKVRVSAKSGKEL"
     gene            6415..6957
                     /gene="rplE"
                     /locus_tag="KRL67_001934"
     CDS             6415..6957
                     /gene="rplE"
                     /locus_tag="KRL67_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018590379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HBH1522813.1"
                     /translation="MASRLQEKYMKEVAPALMEKFGYKNVMEIPKLNKIVINMGIGDA
                     RENPKGLEKGVEELEIISGQKPVITKARKSVANFKLREGMPIGTKVTLRADKMFYFMD
                     KLVSVSLPRVRDFRGVNPNAFDGRGNYALGVKEQLIFPEIEYDKIDKVRGMDIIFVTT
                     AKTDEEARELLKLLGMPFSK"
     gene            6975..7160
                     /locus_tag="KRL67_001935"
     CDS             6975..7160
                     /locus_tag="KRL67_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HBH1522814.1"
                     /translation="MARKAMVVKQQRKQKYATREYTRCTICGRPHSVLKKFGICRICF
                     RELAYKGQIPGVRKASW"
     gene            7193..7591
                     /gene="rpsH"
                     /locus_tag="KRL67_001936"
     CDS             7193..7591
                     /gene="rpsH"
                     /locus_tag="KRL67_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012158382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HBH1522815.1"
                     /translation="MTMTDPIADMLTRIRNANVVKHETVDVPASNMKKELARILLEEG
                     FIRGYDVIEDGKQGIIRIQLKYGQEGERVITGLKKISKPGMRVYAANHEIPKVLNGLG
                     ISVISTSKGILTDKQARKENVGGEVICYVW"
     gene            7622..8164
                     /gene="rplF"
                     /locus_tag="KRL67_001937"
     CDS             7622..8164
                     /gene="rplF"
                     /locus_tag="KRL67_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HBH1522816.1"
                     /translation="MSRIGVKPIIIPAGVEVTIAEGNLVTVKGPKGTLTKQLSAELNI
                     KKEENTIMVERPTNNKKHRSLHGLTRTLLDNMVVGVNTGFEKKLELKGVGYRAQKQGK
                     KLVMNLGFSHPVEMEDPEGITVEAPNQTELIVKGIDKQLVGNYAAKIRAWRKPEPYKG
                     KGIKYVDEVIRRKEGKTGKK"
     gene            8200..8568
                     /gene="rplR"
                     /locus_tag="KRL67_001938"
     CDS             8200..8568
                     /gene="rplR"
                     /locus_tag="KRL67_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000628816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HBH1522817.1"
                     /translation="MLKKADKNANRLQRHKRVRRKISGTSQRPRLCVFRSANNIYAQI
                     IDDTKRVTLVAASSLEAEVKSAVNHTGNKEAAKKVGELVAKKAVEKGITEVVFDRGGY
                     LYHGRIQELAEGAREAGLKF"
     gene            8589..9098
                     /gene="rpsE"
                     /locus_tag="KRL67_001939"
     CDS             8589..9098
                     /gene="rpsE"
                     /locus_tag="KRL67_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011109776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HBH1522818.1"
                     /translation="MLRRKPIDAGQLDLQEKVVEVRRVTKVVKGGRNFRFAALVVVGD
                     ENGHVGIGAGKAMEVPDAIKKAVEDAKKNLIVVPIVGTTIPHEVRGHFGAGNILIMPA
                     VEGTGVIAGGPARAVLELAGLKDVRAKSLGSNNPRNMVNATIEGLNSLKTVEDIAKLR
                     GKKVEELLG"
     gene            9113..9298
                     /gene="rpmD"
                     /locus_tag="KRL67_001940"
     CDS             9113..9298
                     /gene="rpmD"
                     /locus_tag="KRL67_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HBH1522819.1"
                     /translation="MAKLQIKLVRSVIGTTPNQKKNVEALGLRKREQVVVKEDNAQTR
                     GMINKVSHLLEVTEIAE"
     gene            9331..9774
                     /gene="rplO"
                     /locus_tag="KRL67_001941"
     CDS             9331..9774
                     /gene="rplO"
                     /locus_tag="KRL67_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000766080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HBH1522820.1"
                     /translation="MKLHELKPAEGAVRAKRRLGRGTATGQGKTAGRGQKGQWSRSGG
                     GVRVGFEGGQMPLARRLPKRGFNNIFKKVYTEVNVEVLNRFENGTEITAELLKSTKTI
                     SKIGKDGIKILGEGNLEKALTVKAAKFTASAQEKIEKAGGKAELV"
     gene            9818..11086
                     /gene="secY"
                     /locus_tag="KRL67_001942"
     CDS             9818..11086
                     /gene="secY"
                     /locus_tag="KRL67_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HBH1522821.1"
                     /translation="MLSKLKQAWKIKAVRKKVMYTLMMIVIFRIGTTIPVPGIDTSII
                     QKMVGGNSLLSLYNMFTGGAFSNFSLFALGISPYITASIIIQLLTVGFESLAELQKSG
                     EEGKKKINKYTKYTALALAVVQALGITLGIVRSALISNSVFFITTVVITLVSASMLVM
                     WIGDKITEKGIGNGSSVIIFAGIISRIPTDVIKISQQVKSGEVAPWVIVILAVVILLT
                     VTGVTFIQEATRKIPVQYAKRVVGRKMYGGQSSHIPMKVNQSGVMPIIFASSLLAFPQ
                     TIAMFMGPNAQAFVQKYLSMATEQGFWTYRSIEILLIIFFSYFYTTVSFNTEDISKNM
                     KNNGGFIPGIRPGEPTMDYLNRILTRLTLAGATFLAIIAMVPALTTHYMKVNMSLAGT
                     SLLIVVGVALELKRQLESNLVMRSYQGFLK"
     gene            11104..11754
                     /locus_tag="KRL67_001943"
     CDS             11104..11754
                     /locus_tag="KRL67_001943"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HBH1522822.1"
                     /translation="MRIILLGPPGAGKGTQAAGIVEKYNIPHISTGDIFRKNIKEGTE
                     LGKKAKEYMDQGLLVPDELTVGLVTDRISQEDCKNGFMLDGFPRNVAQGEHLDIFLKD
                     AGISLDKVVNIEVDKSILVSRAVGRRICKSCGATYHVEFNPPKVEGVCDVCQGELYQR
                     ADDNEETVSKRIQVYLDETKPLVDYYSKQGIIADIKGDQAIDKVFEDIVAALGSGK"
     gene            11755..12501
                     /gene="map"
                     /locus_tag="KRL67_001944"
     CDS             11755..12501
                     /gene="map"
                     /locus_tag="KRL67_001944"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HBH1522823.1"
                     /translation="MIILKSKKEIELLREAGKIVADTHEVLRKAISPGISTLELDKIA
                     EENIRKYNAEPSFKGYGGFPGSICASINREVVHGIPGETILQEGDIVSLDIGAYYKGY
                     HGDSAKTHGVGMISEEDRKLIEVTRESFYEGIKFAKLGYRLSDISHAVQTHVEKHGFS
                     VVRDLVGHGVGANLHEDPQVPNYGLPGKGPRLREGMVIAIEPMVNAGRYHVKTLSDGW
                     TTVTIDGKKSAHYEHTIAITEHEPLILTKL"
     gene            12523..12792
                     /locus_tag="KRL67_001945"
     CDS             12523..12792
                     /locus_tag="KRL67_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KOW domain-containing RNA-binding protein"
                     /protein_id="HBH1522824.1"
                     /translation="MLSDNLSIGQVVKVSLGRDKGNLFFVVKIINNEYVLIADGKKRK
                     LDKPKLKKVRHLKKYNFINDEVRKRVVSGQEITDSFLRAELTKLN"
     gene            12830..13048
                     /gene="infA"
                     /locus_tag="KRL67_001946"
     CDS             12830..13048
                     /gene="infA"
                     /locus_tag="KRL67_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HBH1522825.1"
                     /translation="MAKKDVIELEGTVSEALPNAMFKVKLENGHEILCHISGKLRMNF
                     IRILEGDKVNVELSPYDLTRGRITWRKK"
     gene            13084..13197
                     /gene="rpmJ"
                     /locus_tag="KRL67_001947"
     CDS             13084..13197
                     /gene="rpmJ"
                     /locus_tag="KRL67_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HBH1522826.1"
                     /translation="MKVRPSVKPICEKCKVIKRKGKVMVICENPKHKQKQG"
     gene            13350..13721
                     /gene="rpsM"
                     /locus_tag="KRL67_001948"
     CDS             13350..13721
                     /gene="rpsM"
                     /locus_tag="KRL67_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005810116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HBH1522827.1"
                     /translation="MARIAGVDLPREKRAEIGLTYIYGIGKATANEILAKAEINPDTR
                     IKDLSEDQVNELRKVIDDDFLVEGDLRREIALNIKRLRDIKCYRGIRHAKGLPLRGQR
                     TKTNARTRKGPRKTVSRKKKK"
     gene            13757..14155
                     /gene="rpsK"
                     /locus_tag="KRL67_001949"
     CDS             13757..14155
                     /gene="rpsK"
                     /locus_tag="KRL67_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006440523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HBH1522828.1"
                     /translation="MAKPKKKVTRIRRRERKNIERGHAHIQSTFNNTIITLTDVHGNA
                     ISWASSGQLGFKGSRKSTPFASQMAAETAAKAAMEHGLKSVEVFVKGPGSGREAAIRA
                     LQATGLEVTMIKDVTPIPHNGCRPPKRRRV"
     gene            14187..14810
                     /gene="rpsD"
                     /locus_tag="KRL67_001950"
     CDS             14187..14810
                     /gene="rpsD"
                     /locus_tag="KRL67_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HBH1522829.1"
                     /translation="MARYTGASCRQCRREGMKLFLKGDRCYTDKCAIVKRNYAPGQHG
                     QGRKKVSNYGLQLREKQKVKRIYGVLETQFRNLYERAENMPGKAGENLLSLLERRLDN
                     VVYRMGLASSRKEARQLVTHGHFTLNGNKVDIPSLIVKVGDVIEVKEKSRSSAKFKNL
                     VEVNSRIAPKWLEANVEGMTAKVVGVPTREDIDLEIAEHLIIELYSK"
     gene            14888..15835
                     /locus_tag="KRL67_001951"
     CDS             14888..15835
                     /locus_tag="KRL67_001951"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HBH1522830.1"
                     /translation="MIEIEKPKVDIVELSEDYRYGKFVIEPLERGYGITIGNALRRIL
                     LSSLPGVAVNAIKIDGVLHEFSTIPGVKEDVTEIILTLKELSATIDGEGSRTLKIEAQ
                     GPCSITGADIICPPDVEILSKDLAIATLDDNAKLNMEIFVDKGRGYVSAEENKTENVP
                     IGVLPVDSIYTPVEKVSYHVENTRVGQKTDYDKLVLEVWTNGSINPQEGISLAAKVLV
                     EHLNLFIDLTEHVSSVEIMVEKEEDQKEKVLEMTIEELDLSVRSYNCLKRAGINTVEE
                     LANKSEDDMMKVRNLGKKSLEEVIQKLEELGLGLKPSEE"
     gene            15856..16197
                     /gene="rplQ"
                     /locus_tag="KRL67_001952"
     CDS             15856..16197
                     /gene="rplQ"
                     /locus_tag="KRL67_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003357477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HBH1522831.1"
                     /translation="MAKYRKLGRETAHRNLMLRNLVTCLLRSGRIETTVTRAKETRRM
                     AEKMITLAKRGDLHARRQVLAYVMDETVVNNLFTDLAPKYAERNGGYTRIIKIGPRKG
                     DAAEMAFIELV"
     gene            16317..17150
                     /locus_tag="KRL67_001953"
     CDS             16317..17150
                     /locus_tag="KRL67_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter ATPase"
                     /protein_id="HBH1522832.1"
                     /translation="MDNIVKVNNISFEYITDEAKLKAIDDLSLDVKKGEFVAIIGHNG
                     SGKSTLSKNLNAILMPTEGNILIDGMDTKEEERLWDIRQTAGMVFQNPDNQIVATIVE
                     EDVAFGPENLGIEPKEIRRIVEESLKSVGMYDLRDRQPHLLSGGQKQRVAIAGIIAMR
                     PKCIIFDEATAMLDPSGRKEVMKTIKRLNKEENITVIHITHFMEEAVEADRVVVMEKG
                     KKILEGTPREVFSKIKMLKEIGLDVPCMTELSSLLIEEGINISSDILTVDEMVMELCQ
                     L"
     gene            17138..18004
                     /locus_tag="KRL67_001954"
     CDS             17138..18004
                     /locus_tag="KRL67_001954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter ATPase"
                     /protein_id="HBH1522833.1"
                     /translation="MSIIVKNLTHIYNEGMPFASKALDDVSFEIKDRDFVGLIGHTGS
                     GKSTLIQHLNGLLKPSSGEIFINDFNITDKNLNLTEIRKRVGVVFQYPEYQLFEETID
                     KDIAFGPSNLGLEESEIHNRVKASMEAVGLEYEEFKDKSPFELSGGQKRRVAIAGVIA
                     MNPEVLILDEPTAGLDPGGRDEIFNLIKDLHEKKNMTIILSSHSMDDMAKLAKTLIVM
                     NHGSVEFMGTPREVFKSNASKLKDIGLDIPQVLELALKLREKGFDISEDILTLEEAKQ
                     EILKVVRGRGLC"
     gene            17998..18801
                     /locus_tag="KRL67_001955"
     CDS             17998..18801
                     /locus_tag="KRL67_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HBH1522834.1"
                     /translation="MLKDITIGQYYPTSSAIHKLDPRIKLVATIVFMVSIFVVNKFWP
                     YIVVLLCLLAMIKLANIPVKYIVKGVKPLKWIILFTFLINIFFLPGDEIWSFGFLAIT
                     KQGLRQAIFMAIRLIFLVVGTSLLTLTTSPIELTDGIERLLNPFKKIGLPVHELAMMM
                     TIALRFIPTLLDETDKIMKAQMSRGADFESKNLINRAKNLVPLLVPLFVSAFRRADEL
                     AMAMEARCYRGGHNRTKMRESVISRRDYMACVFQVVYLGAIIATRFIAI"
     gene            18833..19564
                     /gene="truA"
                     /locus_tag="KRL67_001956"
     CDS             18833..19564
                     /gene="truA"
                     /locus_tag="KRL67_001956"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003514691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HBH1522835.1"
                     /translation="MRNIKITIQYKGKNYCGWQKQPDSLGIQGTIERAIYDITKEETN
                     LIGSGRTDSGVHAIGQIANFKINSGISIESIPMALNAKLPKDISVIEACEVNDDFHSR
                     YSAKGKTYKYLVYNSKFRNPILSEISYQVKYELDFDKMCSEAKSLLGTHDFKGFMSSG
                     SSVKDTVRTIYDIDISKKDDLITFEISGNGFLYNMVRIIVGTLVDMGRGRINEPFLDI
                     IQSKTRSRCGHTAPAQGLFLKKVHY"
     gene            19684..20115
                     /gene="rplM"
                     /locus_tag="KRL67_001957"
     CDS             19684..20115
                     /gene="rplM"
                     /locus_tag="KRL67_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HBH1522836.1"
                     /translation="MKSYIAKPADVQRKWYLVDAEGKTLGRLATEIATVLRGKHKPTF
                     TPHVDGGDFVVVVNAEKIVLSGKKLDQKYYRYHTGYVGGLKEISYRDMMDKKPEEVIS
                     HAVSGMLPKNKLRSRMMTRLRVFAGAEHTHAAQNPEVLNFK"
     gene            20144..20536
                     /gene="rpsI"
                     /locus_tag="KRL67_001958"
     CDS             20144..20536
                     /gene="rpsI"
                     /locus_tag="KRL67_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003515879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HBH1522837.1"
                     /translation="MANVQYYGTGRRKSSVARVRLVAGEGNILVNGRALENYFNYETL
                     IRDVKQPLVLTGNENKYDVIVKVEGGGFTGQAGAIRHGISRALLKADLDLRPALKKEG
                     FLTRDARMKERKKYGLKAARRAPQFSKR"
BASE COUNT         8284 a         2454 c         4336 g         5695 t
ORIGIN      
        1 atggatttaa tataaaaaaa gagggggtaa ctcctctctt ttttgttttt tgttgacaag
       61 tagcgctaaa gcatatatac taaactagtg ccttgagaca gattaacaat caagtttaag
      121 gttatgatgt tgtaatagaa aataaaattg aaaaaaacag aaaaaaaact tgaaaaaatg
      181 taacagatga tataaaatat aatagtgtgc ttgagagatt aagaaaataa taattaaatc
      241 ctagtgaaat acgaactgat atcgatttaa aagttacaac acacccggag cagtgtatcg
      301 gtattggttg tacgcaactt gaaaattaaa cacgtgataa aggagggaaa acaaaatggc
      361 taagaatgaa aaaataagaa taagattaaa atcatatgat cacaaattat tagatttttc
      421 agcaggtaaa atagttgaaa ctgctaaaaa ggctggttca caagtttcag gacctgtgcc
      481 tctaccaaca gaaaaacaag ttgtaactat attaagagct gtacataagt acaaagactc
      541 tagagaacaa ttcgaaataa gaactcataa aagactaatc gacatagcta atccaacacc
      601 taagacagtt gactcattaa tgagattaga cttaccagca ggtgtagata tagaaataaa
      661 attataagaa cccctaagat attatcctat agatgattat cttagaatga ttactctaaa
      721 agatgtaatc cgctgtaaga attataggag gtgctaatat gaaaggaata ttaggcaaaa
      781 aagttggtat gactcaaata ttcactgata aaggtgtagt tatacctgta acagctgttg
      841 aagcagggcc tatggtcgtt actcaaataa aaacagttga taaagatgga tacaatgcta
      901 tacaaatagg ttttgaagat gctaaagaaa aagctttaaa caaacctaaa aaaggacatt
      961 tagctgctgc taatgtttta aagaaacatt taaaagaatt tagagtagat tctgtagaag
     1021 gatacacagt aggacaagaa ataaaagctg atgtatttga agcaggtgca aaaatagatg
     1081 ttactggaat aagtaaaggt aagggattcc aaggtccaat aaaaagacat ggacaaagta
     1141 gaggtcctga aactcacggt tctagatacc acagaagacc aggttcaatg ggagcttgtt
     1201 cttacccagg tagagtattt aaaaacaaaa aattagctgg acacatggga agcgtaaagg
     1261 taacagttca aaacttagag gttgttaaag tagatgctga caagaacctt atattagtta
     1321 aaggagctat accaggagct aaaggttcag tagtaactat aaaagaagct ataaaggttt
     1381 ctaaataatg actaacctag agaaaggagg aataacaatg ccaaaattaa atgtactaaa
     1441 tgttagtgga caaaacgttg gagaaataga attatcagat tctatatttg gcgtagaagt
     1501 taatggacat gttttatacg aagttgtaaa aaatcaatta gcaaataaga gacaaggaac
     1561 tcaatctgct aaaactagag cagaagttag aggtggcgga agaaaacctt ggaaacaaaa
     1621 aggaacaggt agagcaagac aaggttctac aagatctgta caatgggtag gtggaggagt
     1681 tgcttttgca cctaaaccaa gaagctacaa atatacatta cctaagaagg taagaagatt
     1741 agctatgaag agtgctttat cttcaaaagt tcaaaatagt gaagttatag tattagatgc
     1801 attaaacatg gatgctccta agactaaaga atttgctcaa atattaaaca atataaatgc
     1861 tgctaaaaaa gctttagtag ttatagctga caaaaacgat aacgtaataa aatcagctag
     1921 aaatatagaa ggtgttcaaa ctgcattagt taatactatg aatgtatatg atatattaaa
     1981 atacgattca tttataataa ctacagatgc tgttaaaaaa gtggaggagg tgtacgcata
     2041 atgactaatc cacatgatgt aataataaga ccagttgtaa ctgagcacag tatggctgaa
     2101 atgggtgaaa aaaaatacac ttttgttgtg gctaaagatg cgaacaaaac tgaaataaaa
     2161 aaagcagtag aaaaagtatt cggagttagt gttgataaag taaatacttt aaactacgat
     2221 ggaaaagtta aaagaatggg tagaactcaa ggaagaacag caagctttaa gaaagcagta
     2281 gttaaattaa ctgctgatag taaagaaata gaattcttcc aaggaatgta gtattataac
     2341 aaacgaagga gggaaaacag atggctataa aaaagtttag accaacttct cctgccttaa
     2401 gacaaatgac agttttagtt tctgatgaaa taacatgtaa tcaaccagaa aaatctcttt
     2461 tagttaactt aaagaaaaat gctggtagaa acgtacatgg tagaataact gttcgtcata
     2521 gaggtggagg acaaaaaaga aaatatagaa taatagattt taaaagagat aaagatggta
     2581 tacctgctaa ggttgctact atagaatatg atccaaacag aactgcaaat atcgcattat
     2641 taaactatgc tgatggagaa aaaagatata tattagctcc agttggaatt aatgttggag
     2701 atactatatt atcaggatta ggtgctgata taaaaccagg aaactgttta gcattaaaag
     2761 atatgccagt tggtactatc attcataata tagaattaaa gccaggaaaa ggagctcaat
     2821 tagttagatc tgctggagtt tctgcacaat taatggctaa agaaggaaaa aatgctttat
     2881 taagattacc atcaggtgag atgagattag taagcataaa ctgtaaagct actataggtc
     2941 aagttggaaa tatagagcat ggtaacgtag ttatcggtaa agcaggtaga aaaagacata
     3001 tgggaataag acctactgta agaggttctg taatgaaccc taatgaccat ccacacggtg
     3061 gaggggaagg tagatctcca ataggtagac catctcctgt tactccatgg ggtaaaccag
     3121 ctcttggata caaaactaga aagaaaaata aagcttcaaa taaactaata gtatcaagaa
     3181 gaactaagta gtaaatttat ttttgctcgg aaaggaggaa tataaatgtc aagatcaact
     3241 aaaaaagggc cttttgtcca tgcaagactt ttaaagaaga tagaagctat gaatgctagt
     3301 ggaaataaag aagttataaa aacttggtca agatcttcaa ctgtatttcc acaaatggta
     3361 gaaaacacta tagctgttca tgatggaaga aaacatgttc cagtatatat aacagaagat
     3421 atggttggac ataaattagg tgagttcgtt cctactagaa ctttcaaagg acataaggat
     3481 gacgaaaaat ctaataagag aaaataatta aattatgact aagaaaggag gaataacaat
     3541 ggaagcaaaa gctactgcta aatacgtacg tgtatcacca agaaaagcag gccaaatatg
     3601 tgaccttgtt agaggaaaga atgttgatga agcattagca atattaaagt ttactccaag
     3661 aggagcagct tcaataatag ctaaggttgt aaaatcagct aaagctaacg cagaaaacaa
     3721 tcacgaaatg gatactgaaa aattatatat agcatcaata gttgctaatc aaggaccaac
     3781 aatgaagaga ttcatgccta gagctatggg tcgtgcaact acaataagaa aaagaacttc
     3841 tcatatagag gttgttgtta aagagaaaaa ataattaata gataggaggg aaagtaatgg
     3901 gtcaaaaggt taatccacac ggcttaaggg tcggtgttat aaaagattgg gactcaagat
     3961 ggttcgctac tgataaaaaa gagttcggaa acttattatt agaagaccat aatatacgta
     4021 aattcttaaa gaaaagatta tactcagctg gagttgctaa gatagaaata gaaagatcag
     4081 ctaacaaaat aaaaatggac ttacacgttg ctaagcctgg tgtagttata ggaagagctg
     4141 gtgcaggaat cgaagcatta aaagctgaat tagaaaaaat gacaaagaaa actataatag
     4201 ttaatatagt agaagtaaga agtacagata aaaatgctca attagtagct gaaaatatag
     4261 ctttagctat agagagaagg gttgctttca gaagagcaat gaaacaagct atacaaagag
     4321 caatgaaatc tggtgctaaa gggataaaag tttctgcttc aggaagatta ggtggagctg
     4381 aaatggctag aactgaaggt tatagtgaag gaaatgtgcc actacaaact ttaagagcag
     4441 atatagatta tggtttcgct gaagctgata caacttatgg aaaaatcggt ataaaagttt
     4501 ggatatgtaa tggtgaagtt ttaccaacta gagacggtgt aaatccaaga gaagaaagca
     4561 gaaagagcga tagaagagac aacaagagag ataatagaag aaacgataga agaggaaatg
     4621 acagaagagg aaatgataac agaggaaact acagaggaca aagacctcaa ggtggatcaa
     4681 gacctcaaag aactgaaaac aaaggaaatt aataatagtt aggttcaagg aaggaggaaa
     4741 aatactcatg ttaatgccaa aaagagtaaa gcgtcgtaga gttcatagag gaagcatggc
     4801 tgggcaagct cataagggta ataaagttac ttatggtgag tttggattag tagcattaga
     4861 agcttcttgg ataacttcta atcaaataga agctgccaga atcgcgatga ctagatatat
     4921 aaaaagaggg ggaaaggttt ggataaaaat attccctcat aaaccagtaa caagaaaacc
     4981 agcagagact cgtatgggtg cagggaaagg ttctccagaa tattgggtag ctgtagttaa
     5041 gccaggaaga gttatgtttg aattagcagg tgtttctgaa gataaagcaa gagaagctat
     5101 gagacttgct gcacataaat taccaatcaa atgtaaattt gttaaaaaag aagatttaga
     5161 agtaaagggt ggtgaatagg atgaaagcta aagaattaag agatttaaca agcgaagagc
     5221 taatgaacaa attaaacgac ttcaaaagtg aattatttag cttaagattc caattagcta
     5281 ctggtcaatt agaaaataca gctagaataa agtttgttaa gaaggatata gcaaaagtta
     5341 aaactgttct tgctgaaaga aagttatacg aaactagagc ttaatttgga aaggaggctt
     5401 tttaaacatg gaaagaggaa gaagaaaagt tagaataggc cgtgttgtta gtgataagat
     5461 ggataaaaca atagtagtag ctgttgaaga attcgtacgt catccactat ataataaacg
     5521 tgttaagaga actaagaaat tcaaggctca tgatgaaaag aatatatgta acatcggaga
     5581 tagagttaaa ataatggaaa ctagaccatt atctaaagat aagagattca gactagttga
     5641 agttgttgag aaagttaagt agtttttgaa gaaggaggga ttacgatatg atacaacaag
     5701 aatcacgtct aagagttgct gataactcag gagctaaaga acttttatgt atacgtgttc
     5761 taggcggaag taaaagaaga tatggtaaca taggcgacgt tatagttgct actgttaaaa
     5821 gtgcaacacc tggtggagtt gtaaaaaaag gtaaggttgt taaagctgtt atagtaagaa
     5881 gcaaacaagg cgtaagacgt aatgacggaa gctatatatc ttttgatgaa aatgctgctg
     5941 ttataataaa ggacgataaa actccagtag gaactcgtat attcgggcct gttgctagag
     6001 agttaagaga caatgaattc atgaaaatag tttctcttgc tccagaagta ctataattaa
     6061 ggaggtgcaa taggacatga tgcgtgttaa aaaaggtgac actgttgtag ttatagcagg
     6121 taaagataaa ggtaaaaaag gttcagtttt aaaggtatat cctaaaacta gcaaagtgtt
     6181 agttgaaggt gtaaatgtaa taactaaaca tcaaaaacca agtgctatga accaacaagg
     6241 tggaataata aataaagaag ccccaataca catatctaat gtaatgccat ttgatcctga
     6301 aacaggaaaa ggcgttagag taagatatga agtaaaagat gggaacaaag taagagtatc
     6361 agcaaagagc ggaaaagaat tataataaat attgcttgaa aggagggacc ttaaatggct
     6421 tctagattac aagaaaaata catgaaagaa gttgctcctg ctttaatgga gaaatttgga
     6481 tacaaaaacg taatggagat acctaagtta aataaaatag ttattaacat gggtataggt
     6541 gacgctagag aaaatccaaa aggattagaa aaaggcgttg aagaattaga aataatatca
     6601 ggacaaaagc ctgttataac taaagctaga aaatctgttg ctaacttcaa actaagagaa
     6661 ggaatgccaa taggaacaaa agttacatta agagctgata aaatgttcta ctttatggac
     6721 aaattagttt cagtttcttt accaagagtt agagacttta gaggagttaa ccctaatgct
     6781 tttgatggta gaggaaatta tgctttagga gttaaagaac aattaatatt ccctgaaata
     6841 gaatatgata aaatagataa agtaagagga atggatataa tatttgttac tacagcaaag
     6901 actgacgaag aagctcgtga attattaaaa ttattaggaa tgccattttc taagtaagca
     6961 aaggagggat tcccgtggct agaaaagcga tggttgttaa acaacaaaga aagcaaaagt
     7021 acgctactag agaatacact agatgtacta tatgcggaag accgcattca gttttgaaaa
     7081 aatttggtat atgccgtata tgctttagag aattagctta taaaggtcaa atacctggtg
     7141 ttagaaaagc aagctggtag aagcttgatt agaatggaag gagggcaaca aaatgacaat
     7201 gacagatcca attgcagata tgcttactcg tataagaaat gctaatgttg ttaagcatga
     7261 aactgtagat gttccagctt ctaatatgaa gaaagaatta gctagaatct tattagaaga
     7321 aggtttcata agaggttatg atgttataga agatggaaaa caaggtatca taagaataca
     7381 attaaagtac ggacaagaag gcgaaagagt tataacaggt cttaagaaaa tatctaagcc
     7441 tggaatgaga gtttacgctg ctaaccatga gataccaaaa gtattaaatg gattaggaat
     7501 atcagttata tctacttcaa aaggaatttt aactgacaag caagctagaa aagaaaatgt
     7561 tggtggagaa gtaatctgct acgtttggta attaatatga acaatgtaag gaggtgcgac
     7621 catgtcaaga ataggtgtta agccaataat aataccagca ggtgttgaag ttactatagc
     7681 tgaaggaaat ttagttacag taaaaggtcc aaaaggaact ttaactaaac aattaagtgc
     7741 tgagttaaac ataaaaaaag aagaaaatac aataatggtt gaaagaccaa ctaataataa
     7801 aaaacataga tctttacacg gactaactag aactttatta gataatatgg ttgtaggtgt
     7861 aaatactggt ttcgaaaaga aattagaatt aaaaggtgtt ggatatagag ctcaaaaaca
     7921 aggaaagaaa ttagttatga acttaggttt ctctcatcca gttgagatgg aagatccaga
     7981 aggaataact gttgaagctc caaaccaaac agagttaata gtaaaaggta tagacaagca
     8041 attagtaggg aattatgctg ctaagataag agcttggaga aagccagagc catacaaagg
     8101 taaaggtata aaatacgttg atgaagtaat cagacgtaaa gaaggaaaaa ctggtaaaaa
     8161 ataataccaa aacttaaacg ctagaaagga gtgagctagg tgttaaaaaa ggctgataaa
     8221 aatgctaata gacttcaaag acataagaga gttcgtagaa aaataagcgg tacttctcaa
     8281 agaccaagat tatgtgtatt tagaagtgct aacaatatat atgctcaaat aatagatgac
     8341 acaaaaagag taactctagt tgctgcatct tctttagaag cagaagttaa gagtgctgtc
     8401 aatcatacag gaaacaagga agcagctaag aaagttggag aattagttgc taaaaaagct
     8461 gttgaaaaag gaatcactga agttgtattt gacagaggtg gatacttata tcatggaaga
     8521 attcaagaat tagctgaagg tgctagagaa gctggtctta agttctaata cgaaggaggg
     8581 aaaaatacat gctacgtcgt aagccgatag atgcaggaca acttgactta caagaaaaag
     8641 ttgttgaagt aagacgtgtt actaaggttg taaaaggtgg tagaaatttc agatttgcag
     8701 cattagtagt tgttggagat gaaaacggac acgttggtat aggtgctggg aaagcaatgg
     8761 aagtaccaga cgctataaaa aaagcagttg aagatgctaa gaaaaatcta atagtagtgc
     8821 caatagttgg gactacaatt cctcacgaag tacgcggaca ctttggtgct ggaaatatat
     8881 taataatgcc tgctgtagaa ggtacaggag taatagctgg gggacctgct agagccgtac
     8941 ttgagctagc tggactaaaa gacgttagag ctaaatcttt agggtctaac aatccaagaa
     9001 atatggtaaa tgctacaata gaaggactta attctctaaa aacagttgaa gatatagcta
     9061 aacttagagg taaaaaagtt gaagaacttc tagggtaagg aggtagtgta aaatggctaa
     9121 attacaaata aagttagtta gaagtgttat aggaactact cctaaccaaa aaaagaatgt
     9181 tgaagcgtta ggattaagaa aaagagaaca agttgttgta aaagaagaca atgctcaaac
     9241 aagaggtatg ataaataaag taagtcactt attagaagta actgaaatag ctgaataatt
     9301 tttataaagt agtgcaagga ggtgcaatcc atgaagttac atgagttaaa acctgctgaa
     9361 ggtgcagtaa gagctaagag aagattaggt agaggtactg caactggaca aggtaaaact
     9421 gcaggacgtg gacaaaaagg tcaatggtct cgttctggtg gtggagtaag agtaggattt
     9481 gaaggtggac aaatgccact agctagaaga cttcctaaga gaggtttcaa taacatattt
     9541 aagaaagttt acacagaagt taatgttgaa gttttaaaca gatttgaaaa tggaacagaa
     9601 ataactgcag aattattaaa atctactaaa actataagca aaataggtaa agatggaatt
     9661 aaaatcttag gtgaagggaa tttagagaaa gctttaactg ttaaagctgc taaatttaca
     9721 gcttcagctc aagaaaaaat agaaaaagct ggtggaaaag cagagttagt ataataacct
     9781 gctaatctcc tagccagtac aggcggggtg aattagcgtg ctgtcaaaat taaaacaagc
     9841 ttggaagatt aaagcagtaa gaaaaaaagt aatgtataca cttatgatga tcgtgatatt
     9901 cagaataggt actactatac cagttccagg catagacact agtattatac aaaagatggt
     9961 aggcggcaat agtctacttt ctctgtataa tatgtttact ggtggagcat ttagtaactt
    10021 ttcactattt gctttaggta taagtccata tatcactgca tcaatcataa tacaacttct
    10081 aacagtaggt tttgaaagtt tagcagaact tcaaaaatct ggtgaagaag gaaagaaaaa
    10141 aatcaataag tacactaaat atacagcact agctctagca gttgtacaag ctttgggaat
    10201 tacattagga attgtaagaa gtgctttaat atcaaatagt gtgtttttta tcacaactgt
    10261 tgtaataaca ttagtttcag caagtatgtt agtaatgtgg attggggaca aaattactga
    10321 aaaaggaatc ggtaatggta gttcagtaat aatatttgca ggtataatat caaggatacc
    10381 tacagatgtt attaaaatat cgcaacaagt taaatctggc gaagttgcac cttgggttat
    10441 agtgattcta gctgtagtta tattacttac agtaacagga gttacattta tacaagaagc
    10501 aactagaaag atacctgtac aatatgctaa aagagttgtt ggaagaaaaa tgtatggagg
    10561 acaaagttct catataccaa tgaaagttaa ccagtcagga gttatgccta taatttttgc
    10621 tagttcactt ttagctttcc ctcaaacaat agctatgttt atgggtccaa atgctcaggc
    10681 ttttgttcaa aaatacttaa gtatggcaac agaacaagga ttttggacat atagatcaat
    10741 tgagattcta cttataattt tcttctcata tttttataca actgtatcat ttaatacaga
    10801 agatatatct aaaaatatga aaaataatgg tggatttata ccaggtataa gaccaggtga
    10861 acctactatg gattatttaa atagaatttt aactagatta actttagctg gagctacttt
    10921 cttagcaata attgctatgg tgccagctct tacaacacat tatatgaaag taaatatgag
    10981 tctagctggg acttcattgc taatcgttgt tggagttgct ttggagctta agagacagtt
    11041 agagtcaaac ttagttatga gaagctatca aggtttctta aaataaaatg gagatgggat
    11101 aatatgagaa taatattact tggacctcca ggtgctggta aaggtactca ggcggcagga
    11161 atagtagaaa aatacaatat acctcatata tcaacaggag atatattcag aaagaatata
    11221 aaagagggaa cagaacttgg aaaaaaagct aaagaataca tggaccaagg tttattagta
    11281 ccagatgagt taactgtagg tttagttact gatagaatat ctcaagaaga ttgtaaaaat
    11341 ggatttatgt tagatggatt tccaagaaat gtagcacaag gagaacattt agatatcttc
    11401 ttaaaagatg ctggtatatc actagataaa gttgtcaata ttgaagttga taagagtata
    11461 ttagtgtcta gagcagttgg tagaagaata tgtaagtctt gtggagctac ttaccatgtt
    11521 gagtttaatc ctcctaaagt agaaggtgta tgtgatgtat gccaaggaga attatatcaa
    11581 agagctgatg ataatgaaga aactgtatct aagagaatac aagtttatct agatgaaact
    11641 aagcctttag tagattatta tagcaaacaa ggtataatag cagatataaa aggtgatcaa
    11701 gcaatagata aagtatttga agatattgtc gcagccttag gaagtggaaa ataaatgatt
    11761 atcttgaaat caaaaaaaga aatagaactt ttaagagaag caggcaaaat agttgctgac
    11821 actcatgaag ttttaagaaa agctatttct ccgggaatat ctactttaga attagataaa
    11881 atagctgaag agaatataag aaaatacaat gcagaacctt cttttaaagg atatggaggt
    11941 tttccaggaa gtatatgtgc ttctatcaat agagaggtag tacatggtat tccaggagaa
    12001 actatattgc aagaaggaga cattgtaagt ttggatatag gagcctatta taaaggatat
    12061 catggtgatt ctgcgaaaac tcacggagta ggcatgatat ctgaagaaga taggaaattg
    12121 atagaagtaa ctagagaaag cttctatgaa ggaataaaat ttgctaaatt aggatataga
    12181 ctttcagata tctcacacgc agtacaaaca cacgttgaga aacatggttt ctcggtagtt
    12241 agagatttag tgggtcatgg agttggagca aatcttcatg aagacccaca agtacctaat
    12301 tatggactcc caggaaaagg accaagactt agagaaggaa tggttattgc tatagagcca
    12361 atggttaatg caggtcgtta tcatgtaaag actctatcag atgggtggac gactgttaca
    12421 atagatggaa aaaaatcagc tcattatgag catacgatag caattactga acatgaacct
    12481 ttgatattga caaagttgta atttaaaaag taggtgaaaa aagtgctatc agataattta
    12541 agtataggtc aagttgttaa agtttcatta ggacgagata aaggaaattt attctttgta
    12601 gtcaaaataa ttaataatga atatgtatta atagctgatg gaaaaaagag aaaacttgac
    12661 aaacctaagt taaagaaagt gaggcactta aaaaaatata attttattaa tgatgaagtt
    12721 agaaaaagag tagtatctgg acaagagatt acagattctt ttttaagagc tgaacttact
    12781 aagttaaatt agataattta acttattgaa atggagggct ggttagttaa tggccaaaaa
    12841 agatgttata gaattagaag gtacagtttc agaagcttta cctaatgcta tgttcaaagt
    12901 taaactagaa aatggacatg agatattatg ccacatttct ggaaagctaa gaatgaactt
    12961 cataagaatt cttgaaggtg acaaggtgaa tgttgaactt tctccatatg accttacaag
    13021 aggaagaatt acttggcgta agaagtagac tatattaata tttagtctaa ggagggatta
    13081 ataatgaagg ttagaccatc agtaaaacca atatgtgaaa aatgtaaagt aataaaaaga
    13141 aaaggaaaag taatggttat ctgtgaaaat ccaaagcata agcaaaagca aggataagaa
    13201 attattacta gcattttgtt aaattttata gtataatact atattgtgat gttaacaaaa
    13261 gaacgttaaa atcgttgaac aatctggcgt aggattggca tatgatgtga agttttaaac
    13321 actaaaaact atgtgtagga ggtgcgatca tggcaagaat agctggggta gatttaccta
    13381 gagaaaaaag agcggagata ggcttaactt atatatatgg tataggtaaa gcgactgcta
    13441 acgaaatatt agctaaggct gagataaatc cagatactag aataaaagat ttatcagaag
    13501 atcaagttaa tgagttaaga aaagttatag atgacgattt cttagttgaa ggtgacttaa
    13561 gaagagaaat tgctttaaac ataaagagat taagagatat aaaatgttat agaggaataa
    13621 gacacgctaa aggtcttcca ttaagaggac aaagaactaa gactaatgct agaactagaa
    13681 aaggtcctag aaaaacagta tctcgtaaaa agaaaaaata aataaataat aactagatga
    13741 aggagggaaa aggaaaatgg ctaaaccaaa aaagaaagtt acacgtatta gaagaagaga
    13801 acgtaaaaat atagaacgtg gtcatgctca tatacaatca actttcaata atacaataat
    13861 aactttaact gacgttcacg gaaatgctat atcttgggca agttctggac aattaggatt
    13921 caaaggatca agaaaatcaa ctccatttgc atctcaaatg gctgctgaaa cagctgcaaa
    13981 agctgcgatg gaacacggac taaaaagtgt tgaggtattc gtaaaggggc caggttcagg
    14041 aagagaagct gcaataagag ctttacaagc aactggacta gaagtaacta tgataaaaga
    14101 cgttactcca atcccacata acggatgcag accaccaaag agaagaagag tgtaattaat
    14161 aagagtacaa taggaggtgt agataaatgg caagatatac aggtgcatca tgtagacaat
    14221 gccgtagaga gggaatgaaa ttattcctta aaggtgatag atgttataca gataaatgtg
    14281 ctatagtaaa aagaaactat gctccaggtc aacatggcca aggaagaaag aaagtttcta
    14341 actatggatt acaattaaga gaaaaacaaa aagttaaaag aatatatgga gttttagaaa
    14401 ctcaatttag aaatttatat gaacgtgctg aaaatatgcc tggtaaagca ggtgaaaact
    14461 tattaagttt attagagaga agattagata atgtagttta cagaatggga ctagcatctt
    14521 ctagaaaaga agctagacaa ttagtgactc atggtcattt cactttaaat ggaaataaag
    14581 tagacatacc ttcattaata gttaaggttg gagatgttat agaagttaaa gagaaatcaa
    14641 gatcttctgc aaaattcaaa aatttagtag aagttaactc aagaatagct cctaagtggt
    14701 tagaagctaa tgtagaagga atgacagcaa aagtagttgg tgttccaaca agagaagata
    14761 tagatcttga gatagcagag cacttaatca tagaacttta ctctaaataa taagcacact
    14821 tttaaaaatt attttaaaat tttgtttacc ctcagtggat tactgaattt taaggagggt
    14881 tttgtccatg atagaaatag aaaagccaaa agtagatata gttgagctta gcgaagacta
    14941 tagatatggt aagtttgtta tagagcctct agaaagagga tatggaataa ctataggtaa
    15001 cgcattaaga agaatattat tatcatcact accaggtgta gcagtgaatg ctataaaaat
    15061 agatggagtt cttcatgaat tttcaacaat acctggtgtt aaagaagatg ttactgagat
    15121 aatattaact ttaaaagagc tttcagcaac tatagatggt gaaggaagta gaacacttaa
    15181 aatagaagct caaggaccat gctctatcac aggtgcagat ataatctgtc ctccagatgt
    15241 tgaaatatta agtaaagatt tagcaatagc tacattagat gataatgcta aacttaatat
    15301 ggagatattt gtagataaag gtagaggtta tgtttctgct gaagaaaata aaacagagaa
    15361 tgttccaata ggtgttttac ctgtggattc aatatatact cctgttgaga aggttagtta
    15421 tcatgtggaa aacacaagag ttggtcaaaa aacagattat gataaattag tactagaagt
    15481 ttggactaat ggtagtataa atcctcaaga gggcatatca ttagctgcaa aggtattagt
    15541 tgagcattta aacttattta tagacttaac tgaacatgta agtagtgttg aaatcatggt
    15601 agaaaaagaa gaagatcaaa aagaaaaagt tcttgaaatg actatagaag aattagattt
    15661 atcagttaga tcatataact gtttaaagag agcgggaatt aatacagttg aagaattagc
    15721 taataaatct gaggatgata tgatgaaggt tagaaaccta ggtaagaagt cattagaaga
    15781 ggtaatacaa aaactagaag aacttggatt gggattaaaa ccaagtgaag aatagcgagc
    15841 aaaggaggga taagaatggc taagtaccgt aaattaggac gtgaaacagc tcacagaaac
    15901 cttatgttaa gaaacttagt aacttgctta ctaagaagtg gaagaataga aactacagtg
    15961 actagagcga aagaaactcg tagaatggct gaaaagatga taactcttgc taagagaggg
    16021 gatcttcatg ctagaagaca agttttagct tatgttatgg atgaaacagt agttaataac
    16081 ttattcacag atttagctcc aaaatatgct gagagaaatg gtggatacac tagaataata
    16141 aaaatagggc caagaaaagg cgatgctgct gaaatggctt ttatagaatt agtatagtaa
    16201 ggaaaggatt aggcttgatg gcttaatctt ttttttattt ttttgactaa ggtgtgatat
    16261 aataatgtgt aaaatgattt ttataagtta ttgcttacta aaaggagtat gagttaatgg
    16321 ataatatagt aaaggtaaat aatatttcgt ttgagtatat tacagatgaa gcaaaactta
    16381 aagcaataga tgatttaagc ctagatgtta aaaaaggaga atttgttgcg ataataggac
    16441 ataatggttc tggtaagtct actttatcta aaaacttaaa tgctattctt atgcccactg
    16501 aaggaaatat tcttattgat ggtatggaca ctaaagaaga agaaagatta tgggatataa
    16561 gacaaactgc aggcatggta tttcaaaatc cagataatca aatagttgct actatagtag
    16621 aagaagacgt tgcatttgga cctgagaact taggtataga accaaaagaa ataagacgaa
    16681 ttgtagaaga gtctttaaaa agtgtaggta tgtatgattt aagagataga cagcctcact
    16741 tgttatcagg aggtcaaaag caaagagttg caatagcagg aattattgct atgaggccta
    16801 aatgtataat atttgatgaa gcaacagcaa tgttagatcc atctgggaga aaagaagtta
    16861 tgaaaacaat aaaaagactt aataaggaag aaaatataac tgttatacat ataacacact
    16921 ttatggaaga agcagtagaa gctgatagag ttgtagttat ggaaaaagga aagaaaatct
    16981 tagagggaac tcctagagaa gtattcagca aaataaagat gttaaaggaa atcggtttag
    17041 atgtaccttg tatgacagag ttatctagtt tattaataga agaaggtata aatattagta
    17101 gtgatatatt aactgtggat gagatggtga tggaattatg tcaattatag taaagaattt
    17161 aacacatata tataatgaag ggatgccttt tgcaagtaag gcacttgatg atgtttcttt
    17221 tgagattaaa gatagagatt ttgttggtct tataggtcat acaggctcag gaaaatctac
    17281 attaatacaa catttaaatg gattactaaa gccttcttca ggagaaatat ttataaatga
    17341 ttttaatata acagataaaa acttgaattt aactgagatt agaaaaagag taggggttgt
    17401 atttcaatat ccagaatacc aattgtttga agaaacgatt gataaagata tagcttttgg
    17461 accttctaat ttaggcttag aggagtctga gatacataac agagtaaaag catcgatgga
    17521 agctgtaggg cttgagtatg aggaatttaa agacaaatct ccttttgaat tatctggagg
    17581 acaaaagcgt agagtagcga ttgccggagt tatagccatg aacccagaag ttctcatatt
    17641 ggacgaacca actgctggtc ttgaccctgg tggaagagat gaaattttta acttaataaa
    17701 agacttacat gaaaaaaaga atatgactat aatactatca tcacatagta tggatgatat
    17761 ggcaaagtta gcaaaaactt taattgttat gaatcatggt agcgtagaat ttatgggaac
    17821 tcctagagaa gtatttaaat caaatgctag taaattaaaa gatataggat tagatattcc
    17881 acaagtttta gagcttgcat tgaaattgag agaaaaaggc tttgacatca gtgaggatat
    17941 attgacttta gaagaagcaa aacaggaaat attaaaagtt gtgagaggac gaggattatg
    18001 ttaaaagata taactatagg gcaatactac ccaacaagtt ctgctattca taaattagat
    18061 ccaaggataa agcttgtagc gacaattgta tttatggtat ctatatttgt agtaaataaa
    18121 ttttggcctt atatagtagt tttattatgt ttattagcta tgataaaatt ggctaatata
    18181 ccagtcaaat atattgttaa gggtgttaaa cctcttaaat ggattatttt gtttacattt
    18241 cttataaata tttttttctt accaggtgat gaaatatggt catttggatt tttagctatt
    18301 acaaaacagg gtctaagaca agcaattttt atggcaataa gattaatatt tttagtagtt
    18361 ggtacatcat tgcttacttt aacaacttca ccaatagaat taactgatgg aatagagaga
    18421 ttgttgaatc catttaagaa aattggtttg ccagttcatg aacttgcaat gatgatgaca
    18481 attgctttac gttttattcc taccttgtta gatgagacag acaaaataat gaaggctcag
    18541 atgtctagag gtgcagattt tgaaagtaag aatcttataa atagagcaaa gaatttagta
    18601 ccacttttgg tgcctttatt tgtgagtgca tttagaaggg ctgatgaatt ggctatggcc
    18661 atggaggcta gatgttacag aggtggacat aatagaacta agatgagaga gtctgttata
    18721 tcaagaaggg attatatggc atgtgtattt caggttgttt atctgggagc aattatagct
    18781 actagattta tagcaattta attaataagc tatagatata atgaggtaaa gtatgagaaa
    18841 tataaaaatt acaattcaat acaagggtaa gaattattgt ggatggcaaa agcaacccga
    18901 ttctttaggg atacaaggta caattgaaag ggctatatat gatataacaa aagaagaaac
    18961 caatcttata gggtctggta gaactgattc aggagttcat gctattggtc agattgcaaa
    19021 ttttaaaatt aattcaggta taagtataga aagtatacca atggcactaa atgccaagtt
    19081 gccaaaagac atatcagtta tagaagcatg tgaagtaaat gatgattttc actccagata
    19141 cagtgcaaaa ggtaaaacat ataaatattt agtatataat agcaagttta gaaatccaat
    19201 acttagtgag atatcatatc aagtaaaata tgaacttgat tttgataaaa tgtgttcgga
    19261 agcaaaaagt ttattaggaa cacatgattt taaaggtttt atgagttctg gttcatctgt
    19321 aaaagataca gttagaacga tatatgatat tgatataagt aaaaaagatg acttgattac
    19381 ttttgagatt agtggtaatg gttttttata taacatggtg aggattattg ttggaacact
    19441 tgttgatatg ggtcgtggta gaattaacga accattttta gatataatac aatcaaaaac
    19501 taggtcaaga tgtggacata cagctccagc acaaggatta tttctcaaaa aagttcatta
    19561 ttaacttgac acaccaggct acatgtatta aaatatatga gaatgtgtta ataagtccac
    19621 ttgccccgga cttgacataa acggtaaaaa aattttatga aaagtagtta aggagggaca
    19681 gttatgaaaa gttatatagc taagccagct gatgtacaaa gaaaatggta cttagttgat
    19741 gctgaaggaa aaacattagg tcgtttagca acagaaatcg cgacagtatt gagaggtaaa
    19801 cataaaccaa catttactcc tcacgttgat ggtggagatt ttgttgttgt agtaaatgca
    19861 gaaaaaatag ttttaagtgg taagaaatta gatcaaaaat actacagata ccatactggt
    19921 tatgtaggtg gattaaaaga aatatcttac agagatatga tggataagaa accagaagaa
    19981 gttatatcac atgctgtaag cggtatgtta cctaaaaata aattaagaag cagaatgatg
    20041 actagattaa gagtatttgc aggtgctgag cacactcatg cagctcaaaa tccagaagtt
    20101 ttaaacttta aataagatta ataggtgaaa ggaggagttt ataatggcta acgttcaata
    20161 ttatggaact ggaagaagaa aaagttctgt tgctagagta agactagttg caggtgaagg
    20221 aaatatatta gtaaatggaa gagcattaga aaattatttt aattatgaga cattaataag
    20281 agatgttaaa caaccattag ttttaactgg taatgaaaat aaatatgatg ttatagtaaa
    20341 agttgaaggt ggaggattca ctggacaagc tggagctata agacatggta tatctagagc
    20401 tttattaaaa gctgatttag atttaagacc tgctttaaag aaagaaggtt tcttaactag
    20461 agatgcaaga atgaaggaaa gaaagaaata cggattaaaa gcagcaagaa gagctccaca
    20521 attctcaaaa agataattac ttattttttt ggaaaagcaa aatcctttat ttactgttat
    20581 tcagaaagta ttggtatttt aaaaatgttg ggttaagtta attattgata tataataaaa
    20641 taataagata tcttattagg atgtttttca ttaagaagag catccttttt aatttgacta
    20701 tttaataaaa aacatatgct aataacattt aaattttatt tttataatat tgtaatttaa
    20761 aagtttata
//
LOCUS       DAEPXF010000064        20226 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain HB27
            SAMN13376389-rid12479423.denovo.068, whole genome shotgun sequence.
ACCESSION   DAEPXF010000064 DAEPXF010000000
VERSION     DAEPXF010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13376389
            Sequence Read Archive: SRR10518432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 20226)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20226)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001069624.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001069624.1
            Genome Coverage       :: 315x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/15/2021 01:54:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,165
            CDSs (total)                      :: 4,076
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 89
            rRNAs                             :: 10, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 10 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 19 of 69
            Pseudo Genes (incomplete)         :: 44 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 10 of 69
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20226
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13376389-rid12479423.denovo.068"
                     /strain="HB27"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="China: Hebei"
                     /collection_date="2017"
     gene            40..1953
                     /locus_tag="KRL67_001959"
     CDS             40..1953
                     /locus_tag="KRL67_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBH1522838.1"
                     /translation="MKYGGIIMKEILVIAPTKGTYEKSIHIVKKNKYTNIDVVFGNLK
                     EGIPLAEKSINHGARIIISRGGTYNMLKATYNIPIVEIKVDAYDIIKSYKEVKNSNEP
                     FGIIGFNNVIYGFDIIEEILNKKITMIEIEKEEEIYDAIEKYRKKGINTYIGDTTVAH
                     IVKRLNCKGILIESREENILRAIQQAEQILEATKDEQKRRLQIEAMTDFVHDGIITVD
                     KNFKITLFNKSAEKIFGIKKENALYHNVVDVIPNTVLPQVVETMEPQIGEIQNIGSTK
                     IITNRVPIIVDGQIQGAVSTFQDSKEISSFEHTIRRSLLNKGLSAKYTFDDIIHASEK
                     TKDCIELAKKYACYDTPMIITGESGVGKELFCQSIHNYSKRKNAPFVAVNCAAIPPSL
                     IESEFFGYEEGSFTGARKKGKAGVFELAHEGTIFLDEISEIPLELQGRLLRILQEKQV
                     MRIGGDKVIPIDVKIICASNKDLKNMMRNGLFRQDLYFRINILTLYLPSLKERKEDII
                     KLSEFFIHKYSNKYNKTPLIITPNIKKVLLEREYEGNIRELEGMMERAVIVESFDSIL
                     LEKISSEYTNNIVDKDNSDFECSNLDLKTLEKKYIYKIYNENNRNTTKTCEILKITRS
                     TLWRKLKEIEDDV"
     gene            2223..3197
                     /locus_tag="KRL67_001960"
     CDS             2223..3197
                     /locus_tag="KRL67_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-3-deoxygluconate permease"
                     /protein_id="HBH1522839.1"
                     /translation="MHILKSVKKIPGGLMVCPLLLGALFNTVCPQSLEIGGFTTSLFK
                     TGAMSILALFCLCNGAQINIRQAGKPLTKGIVLTATKFIIGAVLGIIVSKFIGPKGII
                     GITPLAIVATMTNSNGGLFAALAGEYGDSTDVGAISILSLNDGPFFTMLAFGVTGLAN
                     VPIVALFAALIPILLGFILGNLDEDIREFLAPGTTLLIPFFAFPLGAALNFNQIITAG
                     LPGVVLGLGVTIITGMGGYFVMKLIRAEHPAVGAATGTTAGNAAGTPEALAAIDPTLA
                     TIAAVSTVQVAAAIIVTAICCPMLVSYLDKREKKKKAIFKTNKNIEVN"
     gene            3218..3493
                     /locus_tag="KRL67_001961"
     CDS             3218..3493
                     /locus_tag="KRL67_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UxaA family hydrolase"
                     /protein_id="HBH1522840.1"
                     /translation="MNAIIHANEKDNLVTCVRPLVKGEKVSIDGKSYIVNDNIPVFHK
                     MATEDVKKGDVVYKYGEVIGIATVDIKTGDYVHVHNIESTRGRGDKK"
     gene            3506..4669
                     /locus_tag="KRL67_001962"
     CDS             3506..4669
                     /locus_tag="KRL67_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UxaA family hydrolase"
                     /protein_id="HBH1522841.1"
                     /translation="MKLMGYLRENGQFGIRNHVLIIPTSVCSSETATRIASLVPGAIA
                     IPHQHGCCQIGSDIELTAKTLIGFGKNPNVAAVLVVGLGCDGIQAKDLASEIATTGKK
                     VDYVVIQECGGTLKTVSKGAEIVGKMAREVSKEVRVEFGMSEITLALECGGSDPTSGI
                     ASNPSIGVASNLLVDEGGSSILSETTEVIGAEHLLATRFEDEKMKDKFLKFVSDVEKR
                     AISMGEDLRSGQPTPGNKAGGLSTIEEKSLGCMYKAGNKPFKGALEYADIIPPNKKGL
                     YFMDTPGQDIDSITGMVAGGAQIVIFSTGRGTPTGSPISPVIKITGNSDTYNKMPDNI
                     DINAGRIITDGAKIADIGQEIFNEIVEVCNGKHTKAESLGHREFGIYRISSTF"
     gene            5012..7291
                     /locus_tag="KRL67_001963"
     CDS             5012..7291
                     /locus_tag="KRL67_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HBH1522842.1"
                     /translation="MNFENTKNKKIIATLLLTLLIIVSLTCVTFIMNLNKVTHNETER
                     YLVEVSQSISTTINVKCKSIFRTLYSIGDTYFEINDSEKEVNRYLSKKLKLFEFDWLG
                     IIDSNGNFVSALKDTGNVKGQDFYRKVLQGKETITPVKSQSGTYGILYSVPLYNGDKI
                     VGAVAAWNTIDTMRNLLSVESFNGEGFSQIIDQDGNFIVSSANKNAPKNVVNFFDMIK
                     HQGKQVDNNKLEEMKINLKKNKTGSMHYTLNDGISKAMHYLPLQEENLYLLSIVPTKT
                     ASAKFDLLLNKAIFINILIVILFLLLIILIMFINQKNRAQLIKIAYVDSVTKGYSKAR
                     FDMEAKKIIQSSSSESYSLVSINIQKFKLINDSFGSEAGDKTLKYIHDIISNYLEPEE
                     LLCRVSNDQFNVLIKTIPQKEILKYIENITNDINSFNKNEECKYFIALSIGVYKIDDP
                     NLPLVSIQDRANVARKNNKGNIGNHPLYTCVFYSDLERLKMIKEKEMENRMEEALNNN
                     EFIVYLQPKISLKDNTIVGAEALVRWQDPNKGLIPPDEFIPFFEKNGFITKLDHYVFE
                     KVCSMIRKWIDNGETPIPVSVNFSRANLNNIDFLEKYRMLRNKYNIPAKLLEIELTET
                     LVFENLQKLINIIDQIHEEGFQCSLDDFGSGYSSLNILKEIKVDTLKLDRAFFSSPNA
                     DNIAENHVIESIVNLAKKLNMNSISEGVETILQMDYLKKINCDMIQGYVFSKPLPQEI
                     FEKMTFGKTLEETSMDSFS"
     gene            complement(7386..8726)
                     /locus_tag="KRL67_001964"
     CDS             complement(7386..8726)
                     /locus_tag="KRL67_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HBH1522843.1"
                     /translation="MENQEALRHEKIWILLFRYSTPAIIAMMVTSLYNVVDRAFIGSM
                     EGIGSMAIAGLGVTMPVFTLIIAFGMLVSVGASTRLSIKLGERNREEAEKILGNALTL
                     SIIISLIITILGLVFLEDILFILGASKDSISYAKDYMSVILIGSIFNLVAFSLNNAIR
                     AEGNPKLSARTMIVGCVLNLILDPIFIFVFNLGIKGAAIATVLCQIVVFFWVTHYFIR
                     GKSNLKLKKCNLKLDKDIVKKVFAIGMTPFAMEVAISITHMFTNNSLKVYGGDLAIGA
                     MTALTSILLMFMMPVFGLNQGMQTIISYNYGAKQYERAKKTLILSIIVSVVILSFGFL
                     VVQVFPEVFVGIFNKDSDLMDIAVRGININLITLPIMGISIVGPVYFQCISKVKHSMF
                     LTLLRQFILFIPLVIVLPIQFNLDGVWLAQPIADFIAMIIVLLFLKREFKTSQV"
     gene            complement(8738..9520)
                     /locus_tag="KRL67_001965"
     CDS             complement(8738..9520)
                     /locus_tag="KRL67_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004460318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBH1522844.1"
                     /translation="MNCINTKNLNVSYGNTDIIKNLNLNIPKGKITTIIGANGCGKST
                     ILKTIGRIISPNSGKIYINGEDIYKKKSKEIAKDMAVLPQSPQAPSGLTVSELISYGR
                     FPHKSGFGNSSKEDREIVNWSLEVTGIAEFKDRNMEDLSGGQRQRAWIAMALAQETDI
                     LLLDEPTTYLDLAHQLEILKLLDELNKKQGRTIVMVIHELNNAARFADHMIGIKNGKI
                     VCEGDAYDVMTKDNLKELFNIDAEIATDPRTNKPICITYDMV"
     gene            complement(9535..10557)
                     /locus_tag="KRL67_001966"
     CDS             complement(9535..10557)
                     /locus_tag="KRL67_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HBH1522845.1"
                     /translation="MKNIIKINSKTLLVISILMVLIFITFLLSLNMGSFSIEPMDVIK
                     TFLGQGQRNHEIAIFKLRLPRIVIALLVGSALSTAGVILQGVTKNDLADSGILGINSG
                     AALFVVVYIYLMNGNVYEGMSNLTVFTMPIVALVGALFGAFLIYSLAWKGGINSSRLL
                     LIGIGVNIAFTSILTIFQLKFTTQEFNRVMAWTSGSIWGTSWKYVIAVLPFIVIFMGI
                     TIYKSKYIDVLNLGDEVSISLGVDVEKQRRRLIIYAVILSGVSTAVAGSISFLGLIAP
                     HIGRKIIGPKHKRLIPVSALIGTLLLLIADTISRNLLAPIEIPVGIVVSIIGVPYFIY
                     LMLVND"
     gene            complement(10563..11576)
                     /locus_tag="KRL67_001967"
     CDS             complement(10563..11576)
                     /locus_tag="KRL67_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HBH1522846.1"
                     /translation="MTKLSKKNMTYLLILVSIVVLVFGIVLSITIGAKDMNLSTVIDS
                     LIKMEDGINMRIVKDVRLPRAIAAALVGGFLAVSGAIMQGITRNPIAEPSVIGITQGA
                     TFAISISLVLQKKLPQLIHGSFSVMIFAFIGASISGLFIYFISSKSRGRVNNVKLALA
                     GVALGTLLISLASAISMYFNLSQQLSFWISGGLVGVKWEGIKLLFIAGGIGFILAIIM
                     APRITILSLGEEVAIGLGQKTNTVRFICIVLVILMTGASVSVSGNIIFIGLIVPQIAK
                     GIVGSDYKYIIPSSLVLGAVLLVYTDILSRMINPPYETPVGSITALIGVPIFIYLVRK
                     GEK"
     gene            complement(11704..12642)
                     /locus_tag="KRL67_001968"
     CDS             complement(11704..12642)
                     /locus_tag="KRL67_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBH1522847.1"
                     /translation="MNKIKSLAIFISIITTLVLVTACSDKNTEDKDKSETRVVQSVKG
                     EVKIPSNPKKIVDISGSSEELLLAGYKPVATANVDSYETDKLPSYIREELKGVKIVGH
                     SMMDTMDMEAILEVNPDLIIMSQRQEKIYDQLKEIAPVVMMKDYANDWRSKLTDVSKL
                     FDKEEEAKNWLQKYDEKATKLGKEVIEKNGEKTYLPVLASSGQFMVFSDGGIGTLIND
                     DMKLARPKNMPKQDGITLPMVSMEGLTDIDADHIVVIATESDKKDLENSAIWAQIRAV
                     KDGNVTILDAAPFFSQSYNPIGKELLLESVKNELTK"
     gene            12908..13372
                     /locus_tag="KRL67_001969"
     CDS             12908..13372
                     /locus_tag="KRL67_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016208838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH1522848.1"
                     /translation="MVHLVYCDNSGKKGEKVLDKILAGTKTMVIRGAAGRKIPHSRVF
                     EDELLYFMEKGTAKISAKATVKKVENYVKLTEDEISKILNENQPKLNLSEKQKVRWHK
                     KCLCLVEFENVEEITPLDFDHQGNMDDWLIVEKIEDVVIGTSIPYNYEKSKF"
     gene            complement(13484..13801)
                     /locus_tag="KRL67_001970"
     CDS             complement(13484..13801)
                     /locus_tag="KRL67_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HBH1522849.1"
                     /translation="MDEKMIEISFGIIGFAGDAKGLAFEAIKEAKSGNIDKARECIEK
                     SKESIHKAHKFQTELIQNEANGNKTEISVILIHAQDHLMTAMNFQQLAEEFIDVYERL
                     ESK"
     gene            complement(13814..14545)
                     /gene="chbG"
                     /locus_tag="KRL67_001971"
     CDS             complement(13814..14545)
                     /gene="chbG"
                     /locus_tag="KRL67_001971"
                     /EC_number="3.5.1.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chitin disaccharide deacetylase"
                     /protein_id="HBH1522850.1"
                     /translation="MTKIIINADDFGYCEAVNYGIISAHNNGIVRSTSMMANMPGVEH
                     GVGLLKENKTLNCGVHMTLSCGRPLLSNLKTIVDKDGFFIRRITDEIIEKMDCDEIYR
                     ELCAQIDRVKGLGIDISHLDSHHHIHTLVSLKPVIEKIVIKYNLPIRGGFEYNLEYSK
                     VVPLIDSFYKENVSEEYFIKNIDEIMKYDVVDIMSHPAFLDDYILNSTSYAIDRTKEH
                     KILTSKKVKEFLEKNGLVISSYRDV"
     gene            complement(14571..15881)
                     /locus_tag="KRL67_001972"
     CDS             complement(14571..15881)
                     /locus_tag="KRL67_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HBH1522851.1"
                     /translation="MKNGLKIVTIGGGSSYTPELVEGFINRYEELPVKELWLVDIEAG
                     KEKLEIVGNLAKRMVKKAGVDMKINLTLDRREALRDADFVTTQLRVGLLDARIKDETI
                     PLSHGVMGQETNGAGGLFKALRTIPVIFDIIKDVEELCPNAWMVNFTNPTGIITEAVF
                     KYTNFRRYIGLCNVPIHLKNDVAKLFNVESDRISMDFAGLNHMVYGLNVSLDGEDVTK
                     EAIDKFVKADISMQNIKAIDFNAEFVKSLGAIPCPYHRYYYKTKEMLEDELKEFKEGK
                     ARGQVVKELEEQLFELYKDEKLDVKPPQLEKRGGAYYSDAACNLISSIYNDKKDIQVV
                     NTLNNGSIRDFKDEQAVEVSSVITKNGPKPISIGYLPDSVHGLVSQIKSFEVLAAKAA
                     VYGDYESALLALCINPLIPSDDLAKTILDEMLEAHKDYLPRFNR"
     gene            complement(15979..17292)
                     /locus_tag="KRL67_001973"
     CDS             complement(15979..17292)
                     /locus_tag="KRL67_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBH1522852.1"
                     /translation="MEKFMSFMDKYIVPVAAKIGAQRHLVAVRDAFIVMIPITMVGAL
                     GTLINNLPLEAYKNLMSSIFGENWTTFGGDLWWGAIGTMAVFLVIGVAYFLAKSYESD
                     GLQSGLIALSIFFIMAPQIGKIVPEGGTTVVEGWGMIQQTYLGTAALFSSILIGLLST
                     EIFVRLSKVKKLTIKMPDGVPPAVSRSFAKLIPGMLTIMIFTVIGIFIKMLSNGSFLT
                     DILNTYLGAPLSNVADSLGSTMLIAFIIHILWTVGLHGANIALPFTETILMKLGGENA
                     ALAQAGATEGYHVLAGAFFDAFVYLGGSGMVLGLIVALLIAGRRRKEMIVLGGPPAIF
                     NIGEPLIFGLPIVLNPIFMIPFVLAPVICSAVSYLAIDFGLVAPVILPKIPWVTPPIL
                     GGAMATGDWTGGALALFNLILSILIYIPFVIASEKMEANKLKINN"
     gene            complement(17314..17625)
                     /locus_tag="KRL67_001974"
     CDS             complement(17314..17625)
                     /locus_tag="KRL67_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBH1522853.1"
                     /translation="MIKIMLACSAGMSTSLLVTKMEDAAKENGIEAKIWAISEVNLKN
                     EIENCDVLLLGPQVRYVLGKATEMAKPYNIPVEVINMMDYGRCNGKAVLDRAVELNSS
                     K"
     gene            complement(17825..18529)
                     /locus_tag="KRL67_001975"
     CDS             complement(17825..18529)
                     /locus_tag="KRL67_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBH1522854.1"
                     /translation="MKEPIYKVIENHVRELINSDSLKEGDLIPSEKQLSEEFNVTRMT
                     VRSALNNLVKEGYITRQRGVGSIVIANNIYDNISSVSGFTKEMESKGYKVSNILVSLE
                     IVQADENLSSKLNISLEENVWEIKRVRLANDARVSYMITYMPVKLFPNLNKTHCENSL
                     YNFVEEVCGYKIAMSEREVQAVISNEECMDNLKLEEPEPLLYISQICKLQNSEIFEYS
                     HTYHYGYTLTLNAVVE"
     gene            complement(18624..19985)
                     /locus_tag="KRL67_001976"
     CDS             complement(18624..19985)
                     /locus_tag="KRL67_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HBH1522855.1"
                     /translation="MKDRLNLTEGRITEKLLKLSLPIMGTSFIQMGYNMIDMIWVGKA
                     GSKAVAAVGTAGFFPWLAMAFIMISKVGGEIKVAQSIGANNISTTKSYMKSAIEINII
                     LAIIYTMSLIVLNKQLIGFFRLGDLEVITMSRQYLIIVALGMVFYFINPVFTAIFNGL
                     GNSKTPFRINTVGLITNIILDPILIFGWGFAPKLGVAGAAIATVFAQIVVTTCFIYII
                     VKSKEEYFKLRLFKEIETKYYKVLYKLGLPIAIQSGMFTVFSMLLGVIVASWGPVAVA
                     VQKVGSQIEAISWMTAEGLSVALGSFVGQNYGAKKYSRINKGCKIAITVSSVLGIITT
                     LVLVFAGESIFSIFIDESEAIEKGAIYLKILGYSQFFMCLEIITTGAFKGLGRTYIPS
                     TIITILTGARVPLAYFISRPEILGLNGVWWSITLSSILKGILMISLFISLLKLGKLYK
                     MSE"
BASE COUNT         7113 a         3339 c         2651 g         7123 t
ORIGIN      
        1 gataaaattt agtcataaga taatatttaa gtttatttga tgaaatatgg aggaattatt
       61 atgaaggaaa tactcgttat tgctcccact aaaggaacct atgaaaaatc aattcacata
      121 gttaaaaaaa acaaatatac aaatattgat gttgtatttg gcaatctaaa agaaggtata
      181 cctcttgctg aaaaaagtat taatcatggt gctagaatta tcataagccg aggtggaact
      241 tacaatatgt taaaagctac ttacaatata cctatagttg aaattaaagt agatgcttat
      301 gatattataa aaagctacaa agaggtaaaa aattccaacg aaccatttgg aataattgga
      361 tttaataatg tcatctacgg tttcgatatt atagaagaaa tattgaataa gaaaataact
      421 atgattgaaa ttgaaaaaga agaagaaatt tatgatgcta tagaaaagta cagaaagaaa
      481 ggtataaaca catatatagg agataccact gttgcacata ttgttaagag acttaattgt
      541 aagggaatat taatcgaatc tagagaagaa aacatactta gagccattca acaagcagaa
      601 caaatactcg aagcaaccaa ggatgaacaa aaaagaagat tacagataga agctatgaca
      661 gattttgtac atgatggaat aataacagtt gataaaaatt ttaaaataac attatttaat
      721 aagagtgccg aaaaaatatt tggtataaaa aaagaaaatg cactttatca taatgttgta
      781 gatgttatcc caaacacagt tcttccacaa gtagttgaga ctatggaacc tcaaattggt
      841 gaaatacaaa atataggaag tacaaaaatt attacaaata gagttccaat cattgttgat
      901 ggtcagattc aaggagctgt ttccacattt caagatagca aggagataag ttcttttgag
      961 catacaataa gaaggagttt attaaacaaa ggtctatctg ctaaatacac ttttgatgat
     1021 ataatccatg ctagtgaaaa gacaaaagat tgtatagaac ttgcaaaaaa atatgcatgt
     1081 tatgataccc caatgattat cactggagaa tctggtgttg gtaaggagct attttgtcaa
     1141 agcatacata actatagtaa gcgtaaaaat gcaccttttg ttgctgtaaa ttgcgctgcc
     1201 atacctccat ctttaataga aagtgagttt tttggttatg aggaaggttc atttacaggt
     1261 gctagaaaaa aaggtaaagc aggtgtattc gagttagcac atgaaggtac aatcttccta
     1321 gacgaaataa gtgaaatccc tcttgaactt caaggtcgat tattgaggat attacaggaa
     1381 aaacaagtta tgagaattgg tggtgataaa gttattccta tagatgtaaa aattatctgt
     1441 gcttcaaata aagacttaaa aaatatgatg cgcaatggtt tatttagaca agacctttat
     1501 tttagaataa atatattaac attatacctt ccttcattaa aagaaagaaa agaagatatt
     1561 ataaaattat cagaattttt tatacataaa tattcaaata aatataataa aacgccttta
     1621 attatcactc caaatattaa aaaagtactt cttgaacgag aatatgaagg taatataagg
     1681 gaattggaag gtatgatgga aagggctgtt attgtggaat catttgactc tattttatta
     1741 gaaaagatta gtagtgaata tacaaataat atagttgata aagataatag tgattttgaa
     1801 tgcagtaatt tagatttaaa aactttagaa aaaaaatata tctataaaat ttataacgaa
     1861 aataatagaa atactacaaa aacctgtgaa attctaaaga taactcgctc cactctatgg
     1921 cgtaagttaa aagaaataga agatgatgtt taaaaataat acatatctat atatatcaaa
     1981 acgttttatg tttaatttta atacataaga cgtttatttg ttttcaataa aaaatacaat
     2041 tatatgataa aaagaatctt tataagtgaa attctcgtca atcatacttt taattataag
     2101 tattctacag catttcaatc gattattaaa aataatcatg ataactaaag atataaactc
     2161 tgtatattgg catagtaatt gcttttagat acctatataa aaatgaaagg gagtgatact
     2221 agatgcacat cttaaaatca gtgaaaaaga taccaggagg acttatggtc tgtcccttgt
     2281 tattgggagc actatttaat acagtatgtc cacaatctct tgaaattgga ggattcacaa
     2341 catccttatt taaaactggt gcaatgagta tacttgctct attttgtcta tgtaatggag
     2401 ctcaaatcaa tatcaggcaa gctggtaagc cattgactaa aggtatagta cttacagcaa
     2461 caaaatttat aattggtgca gtattaggta ttattgttag taaatttatt ggacctaaag
     2521 gtataattgg tattacacct cttgccatag ttgctaccat gactaattca aatggaggtc
     2581 tatttgctgc tcttgcaggt gaatatggag actccacaga tgttggagca atatctatct
     2641 tatctctaaa tgatggtcct tttttcacaa tgcttgcttt tggagtaact ggacttgcaa
     2701 atgtacctat agtagcacta tttgcagcac ttataccaat actacttgga tttatacttg
     2761 gtaaccttga tgaagatata cgagagtttc ttgcacctgg tacaacatta ttaattcctt
     2821 tcttcgcttt tcctttagga gcagcattaa actttaatca aattataact gctggactgc
     2881 ctggagttgt acttggttta ggagttacta taataacagg tatgggtggg tactttgtaa
     2941 tgaaacttat acgtgcagaa catccagcag taggtgcagc tacaggtaca acagccggaa
     3001 atgcagctgg tactcctgaa gctttagcag ctatagaccc tactctagct acaatagcag
     3061 ctgtttcaac agtacaggtt gctgctgcta ttattgttac agcaatatgt tgtccaatgt
     3121 tagtttccta tctagataaa agagaaaaaa agaaaaaagc tatttttaaa acaaataaaa
     3181 atattgaagt aaattaatac ttaaggaggt aaaattaatg aatgcaatta ttcatgcaaa
     3241 tgaaaaagat aatctagtta catgtgtcag acctttagtt aaaggtgaaa aagtttcaat
     3301 tgatggaaag tcttacatag taaatgataa tatacctgtc tttcataaga tggctacaga
     3361 agatgttaaa aaaggtgatg ttgtatacaa atacggtgaa gttataggaa ttgcaacagt
     3421 tgacataaaa actggtgact atgttcatgt acataacatt gaaagtacac gtggtcgtgg
     3481 agataaaaaa taggaggaag ttaaaatgaa attaatgggt tatttaagag aaaatggtca
     3541 gtttggtatt cgtaatcatg ttttaattat tccaacatct gtatgttcaa gtgagacagc
     3601 tacaagaata gcatcacttg tgcctggagc aatagctatc cctcatcaac atggatgttg
     3661 tcaaattggc tctgatatag agcttacagc aaagacatta ataggatttg gaaaaaatcc
     3721 taatgttgcc gcagttttag tagttggtct tggatgtgat ggtattcaag caaaagactt
     3781 agcatctgaa atagctacaa caggaaaaaa ggtagattac gttgttatac aagaatgtgg
     3841 tggaacatta aaaacagttt ctaagggagc tgaaatagta ggtaaaatgg ctcgtgaagt
     3901 atcaaaagaa gtaagagttg aatttggtat gagtgaaatt acattagcac ttgaatgtgg
     3961 tggttctgac ccaacaagtg gaatagcttc aaatccttca ataggcgttg catcaaacct
     4021 tcttgtagat gaaggtggaa gtagcatact aagtgaaact acagaagtaa ttggtgcaga
     4081 acatttattg gctactcgtt ttgaagatga aaaaatgaag gacaaatttc ttaaatttgt
     4141 aagtgatgta gaaaaaagag caatttctat gggggaagat ttgcgtagtg gtcaacctac
     4201 tccaggcaat aaagctggtg gactttcaac aattgaagaa aaatcacttg gatgtatgta
     4261 taaggcagga aataagccat tcaaaggtgc tcttgaatat gctgatatta tacctcctaa
     4321 taaaaaagga ttatatttta tggatactcc aggtcaggac atagattcta tcactggtat
     4381 ggtagcaggt ggtgcacaaa ttgtaatctt ttcaactggt agaggtactc caacaggaag
     4441 ccctatttca ccagttatta aaatcactgg aaatagtgat acatacaaca agatgccaga
     4501 caatatcgat ataaatgcag gtagaattat aacagatggt gctaaaatag cagatatagg
     4561 tcaagaaata tttaatgaaa tagtagaagt atgtaatggt aagcatacca aagctgaaag
     4621 tcttggtcat agagaatttg gtatttacag aatttcatct acattttaga ttgcaataga
     4681 caataaaaca ttttttataa atataatact tatatagatg tataaactaa aagtcctttc
     4741 taatcttata aaaatttaca atactgaaaa ggaactgtat caaagttatt ttgagaacag
     4801 ttccttttta gcatctttta cttaaaacat cataaaatac gatttaaaac tataaacata
     4861 tcttatctac tctctaaaaa gcttaaataa agttaaatat aaagtcaaat aaataaaatt
     4921 taatatcttt gtatgaaaag atatttggta taatatgtct gagagaattc ttatttgtaa
     4981 acaaataaag gatggaggat gaaataaaaa aatgaacttt gaaaacacaa aaaataaaaa
     5041 aataatagct accttactat tgacacttct tataatcgtg tccttaacat gtgtaacttt
     5101 tattatgaat ctaaataaag taacacataa tgaaacagaa cgatatcttg tagaagtttc
     5161 acaaagtatt tccactacaa ttaatgtgaa atgtaaaagt atatttagga cattatattc
     5221 tataggagat acttattttg agattaatga tagtgaaaaa gaagtcaata gatacttatc
     5281 aaaaaaatta aaattatttg aatttgattg gttaggtatt atcgattcta atggaaattt
     5341 tgtaagtgcc cttaaagata caggtaatgt taaaggacaa gacttttata gaaaagtcct
     5401 tcaaggtaaa gaaaccatta ctcctgttaa atcacaatct ggtacatatg gaattttata
     5461 ttctgtacca ctatacaatg gcgataaaat tgttggagct gttgctgctt ggaatactat
     5521 tgatactatg agaaatttat taagtgtaga aagctttaat ggtgaaggat tttcccaaat
     5581 tatcgaccaa gatggaaatt ttattgtaag ttcagcaaac aaaaatgctc caaaaaatgt
     5641 agtaaatttt tttgacatga ttaaacatca aggaaaacag gttgataaca ataaacttga
     5701 agaaatgaaa ataaacttaa aaaagaacaa aactggttct atgcattata cattaaatga
     5761 tggtatttct aaagcaatgc actatcttcc attacaagaa gaaaacttat acctcctttc
     5821 aattgttcca acaaaaacag caagtgcaaa gtttgattta cttctaaata aagcaatctt
     5881 catcaatatt ttaattgtta tactattttt attacttatt atattgatta tgtttataaa
     5941 tcaaaaaaac agagcacaac ttataaaaat cgcctatgta gactctgtaa ctaaaggtta
     6001 cagtaaggca cgttttgata tggaagctaa aaaaattata cagtcatctt catcagaaag
     6061 ctattcatta gtatctataa atattcagaa attcaaactt attaatgatt cttttggtag
     6121 tgaagcagga gataaaacat taaagtacat acatgatatc attagtaatt atctagaacc
     6181 agaagaatta ctttgtcgag tttctaatga ccagtttaat gtacttatta aaactattcc
     6241 tcaaaaagaa attcttaagt acatagaaaa tatcactaat gatattaata gtttcaataa
     6301 aaatgaagaa tgtaaatatt ttatagccct atctataggt gtatataaaa ttgatgaccc
     6361 aaatctacct ttagtttcaa ttcaagaccg tgcaaatgtt gcacgaaaaa ataataaagg
     6421 aaacattgga aatcatccac tttatacatg tgttttttat agtgatttag aacgcttaaa
     6481 gatgattaaa gaaaaagaaa tggaaaatag aatggaggaa gcattaaata ataacgaatt
     6541 tatagtatat cttcagccta aaatcagcct taaagataat actattgtag gtgcagaagc
     6601 attagtaaga tggcaagacc caaataaagg acttatacca ccagacgagt ttattccttt
     6661 ctttgaaaaa aacggtttta ttacaaaact cgaccactat gtttttgaaa aagtatgtag
     6721 tatgataaga aaatggatag ataatggtga aactccaata cctgtttcag tcaatttttc
     6781 acgtgctaat ttaaataata tagatttttt agaaaaatac agaatgcttc gtaataaata
     6841 caacattcct gctaaactct tagaaattga attaactgaa acccttgtat ttgaaaatct
     6901 ccaaaaacta attaacataa tagaccaaat ccacgaagaa ggttttcaat gttcactaga
     6961 tgactttgga agtggatact cttcactcaa tatattaaaa gaaataaaag ttgacacatt
     7021 aaaacttgac cgtgcttttt ttagctcacc taatgcagat aatattgctg aaaatcatgt
     7081 aatagaatct atagttaatt tggcaaaaaa attaaatatg aatagtatat cagaaggtgt
     7141 tgaaacaatc ttgcaaatgg attatcttaa aaaaattaat tgtgatatga tacaaggata
     7201 tgttttttca aaacctttgc ctcaagaaat atttgaaaaa atgacttttg gaaaaacact
     7261 agaagaaacc agtatggata gtttcagtta aataaaataa aaaacttgtc tctataaaat
     7321 aattttaata attttataaa gacaagtctt tttattttat aatttcattt tactttctag
     7381 atttcttata cttggcttgt tttaaactcc ctttttaaga ataataatac gattatcata
     7441 gcaataaaat ctgcaattgg ctgtgccaac caaacaccat ctaaattaaa ttgaattgga
     7501 agtacaataa ctaatggtat aaataaaata aattgtctta aaagagttaa aaacattgaa
     7561 tgtttaactt tacttataca ctgaaaatac acaggtccta caatagaaat ccccattata
     7621 ggcaatgtaa tcagatttat gtttatacct cttactgcta tatccatcaa atctgaatct
     7681 ttattaaata tgcctacaaa tacttctggg aaaacttgta ctaccaaaaa tccaaatgac
     7741 aatatgacaa ctgacactat gatagataat attaaagttt tctttgcacg ctcatattgt
     7801 tttgcaccgt aattgtatga tattatagtt tgcatacctt gattcaatcc aaatacaggc
     7861 atcataaaca tcaataaaat agatgtaagt gctgtcatag ccccaatagc taaatctcca
     7921 ccataaacct ttagagaatt atttgtaaac atgtgagtta tacttatagc tacttccata
     7981 gcaaaaggtg tcatgcctat tgcaaatacc ttctttacta tatctttatc caattttaag
     8041 ttacattttt ttagttttaa attggacttt cctcttataa aataatgtgt tacccaaaag
     8101 aatactacta tttgacagag tactgttgct atagctgctc cttttatacc taaattaaac
     8161 acaaatataa atatagggtc caatatcaag tttaatacgc atcctacaat cattgttctt
     8221 gctgataatt ttggattacc ttctgcccta attgcattat taagagaaaa tgcaactaaa
     8281 ttaaatatac ttccaatcaa tatgacactc atatagtctt ttgcataaga tattgaatct
     8341 ttacttgcac ccaaaataaa aagtatatct tctaagaaaa ctaaccctaa tatagttata
     8401 attaaagaga taattataga taatgtcaaa gcattaccta atatcttctc tgcttcttct
     8461 ctatttcttt cccctaactt tatagacaat cttgtgctag cacctactga tactaacatt
     8521 ccaaaagcta ttattagagt aaatactggc atagttacac caagacctgc aatagccata
     8581 gaaccaatac cttccataga accaataaaa gctctatcta ctacattata aagtgaagtt
     8641 accatcattg ctattattgc aggtgtcgaa tacctaaata ataaaatcca tattttttca
     8701 tgtcttaatg cttcttgatt ctccatgttg tcaccattta caccatgtca taagttatac
     8761 aaattggctt atttgttctt gggtctgttg ctatttctgc atcaatatta aataattctt
     8821 ttagattatc tttcgtcata acatcatatg catcaccttc acaaactatt ttaccattct
     8881 taatgcctat catgtggtca gcaaatcttg ctgcattatt aagttcatgt attaccatga
     8941 caatagttct tccttgtttt ttatttagtt catctaataa ttttagtatc tctaattgat
     9001 gtgccaaatc taaatatgtt gtaggctcat ccagtaataa tatatcagtc tcttgtgcca
     9061 aagccatagc tatccatgcc ctttgtcttt gtcctcctga taaatcttcc atatttctat
     9121 ccttaaattc tgcaattcca gtaacttcta aactccaatt tacaatctct ctgtcttctt
     9181 tactagaatt accaaatcca cttttatgag gaaatcttcc ataagaaatt aattctgaaa
     9241 cggtaagacc actaggagct tgagggcttt gtggaagtac agccatatcc tttgctattt
     9301 ctttagactt ctttttgtat atatcctctc catttatata tatttttccg ctattagggc
     9361 taataattct acctatagtt ttaagtattg tcgattttcc acaaccatta gcccctatga
     9421 tagtagttat ttttcctttt ggtatattta aatttaaatt ttttataata tctgtatttc
     9481 catatgatac atttagattc ttagtattta tacagttcat aacaacaccc tttcctaatc
     9541 atttaccaac attaaataaa taaagtatgg cactcctatt atagaaacta ctatacccac
     9601 tggtatttct ataggtgcta gaagatttct tgatatagta tctgctatta aaagtaataa
     9661 tgttcctatc aatgctgata ctggtattaa tcttttatgc tttggcccta ttatttttct
     9721 acctatatgt ggagctatta aacctaaaaa tgaaatacta cctgctactg ctgtcgatac
     9781 accagataaa attactgcat atattataag tcttcttctt tgtttttcta catctacacc
     9841 aagacttata gatacttcat cacccaaatt taaaacatct atatatttag atttataaat
     9901 agttatcccc ataaaaatca ctataaatgg aagcacagct attacgtatt tccaactggt
     9961 tccccaaatg cttcctgatg tccatgccat aaccctatta aattcttgtg ttgtaaattt
    10021 caattggaat atcgtcaaaa ttgaagtaaa agctatattt acacctatac caataagtag
    10081 taatcttgac gagtttatac cacctttcca tgcaagagaa tatataagaa atgctccaaa
    10141 taaagctcct acaagtgcaa caataggcat tgtaaataca gtcaagttac tcataccttc
    10201 atatacatta ccattcataa ggtatatata aactactaca aataatgcag cacctgaatt
    10261 tattcccaaa attccagaat cagctaaatc attctttgta actccttgaa gtattacacc
    10321 tgctgttgat aatgcagaac caaccaaaag tgctattact attcttggta atcttaattt
    10381 aaatattgca atttcatgat tacgctgacc ttgccctaaa aaagtcttta taacatccat
    10441 tggttctata gaaaatgaac ccatatttaa actcaataaa aaagtgataa aaataagcac
    10501 cattaaaatt gatataacaa gtaatgtttt agaatttatt tttataatat ttttcatagc
    10561 acctatttct ccccctttct aacaagatat ataaatattg gcactcctat aagtgctgtt
    10621 atagaaccta ctggtgtctc atatggagga tttatcatcc tacttaatat atctgtatat
    10681 acaagcaata cagctcctaa tactaaagaa ctcggtatta tatacttata atctgaacct
    10741 actattcctt tagctatttg aggaactata agacctataa aaataatatt accagacact
    10801 gacacagatg ctccagtcat taatattaca agtactatac atataaatct aacagtgtta
    10861 gttttttgtc ctaatcctat tgccacttct tctccaagac ttaatatagt tattcttgga
    10921 gccataataa tagctaaaat aaatccaata ccaccagcta taaatagcaa ttttatacct
    10981 tcccacttca ctcctacaag ccctcctgat atccagaaac ttaactgttg agataggtta
    11041 aaatacatag atatcgctga tgccaaagaa attagcaatg taccaagagc aactcctgca
    11101 agagctaatt ttacattatt aactcttcct cttgatttag aacttataaa atatataaat
    11161 aatccactta tacttgctcc aataaatgca aatatcatta cactaaaact tccatgtata
    11221 agttgaggaa gttttttttg aagtaccagt gatatcgaaa ttgcgaatgt agctccttgt
    11281 gtaattccta taacagatgg ttcagctata ggatttctcg ttattccttg cattattgcc
    11341 ccggaaactg ctaaaaatcc tccaacaagt gcagctgcta ttgcccttgg aagtctaaca
    11401 tcctttacta ttctcatatt tattccatct tccattttta tcaagctatc tataacagtt
    11461 gatagattca tatcttttgc cccaatagtt atggataata caattccaaa tacaagtaca
    11521 actatactta ctaatatcaa caaataagtc atattttttt tactcagctt agtcaactat
    11581 cctacctcct tttccctttt aatcgtctgt ataataccat ctattaagta ttttaatttt
    11641 aagatttatt aaaaataggt gtgaaaaatg aatatcatta tctcacacct actaagttaa
    11701 gaattatttt gttaattcat ttttaactga ttctaataat aactcttttc caattggatt
    11761 gtatgattga ctgaaaaatg gagcagcatc aagtattgtt acattaccat cttttactgc
    11821 tcttatttga gcccagatag cactattttc taaatctttc ttatctgatt ctgtagcaat
    11881 tacaactata tggtctgcat ctatatctgt taaaccttcc atacttacca ttggtagtgt
    11941 tattccatcc tgttttggca tattcttagg tctagctaat ttcatatcat cattaattag
    12001 agtacctatt ccaccatcac taaataccat aaattgacct gaacttgcca acactggtaa
    12061 ataagttttt tctccattct tttctataac ttctttacct aactttgtag ctttttcatc
    12121 atacttctgt agccaatttt tagcttcttc ttctttatca aataactttg aaacatcagt
    12181 taacttgctt ctccagtcat ttgcataatc tttcatcata actacaggag ctatttcttt
    12241 taattggtca tatatttttt cttgtctttg actcattatt attaaatcag gatttacttc
    12301 taatatagct tccatatcca tagtatccat cattgagtgc ccaactatct ttacaccttt
    12361 aagctcttct cttatgtaac ttggtaattt atcagtttca tatgagtcta catttgctgt
    12421 tgcaacaggc ttataacctg ctaataatag ttcttcacta cttcctgata tatcaactat
    12481 ttttttagga tttgatggta tcttaacctc tcctttaaca gattgtacaa ctcttgtctc
    12541 tgatttatct ttatcttctg tattcttatc tgaacatgct gtaacaagta ctaacgtagt
    12601 tataatagat ataaatattg caagtgattt tattttattc atgattattt ctcctacatg
    12661 tttattatac ttatgtgata ttgattatca taagcacata tttattatag catttttcta
    12721 tttttagtca atattatttt gtaaaaggat aaaaattcct aattaacctt aattatatat
    12781 agcttagata aggttttctt atattaaaca gtattttttt taaaaattgg atataaatac
    12841 aatgactttt aagttaaaca atgataaaat gctatagata aggaatttag taaggagggt
    12901 tcaaaacatg gtacatttag tatattgtga taattcagga aaaaagggag aaaaggtctt
    12961 agataaaatc ttagctggaa caaaaacaat ggttattaga ggtgctgctg gtagaaaaat
    13021 accacatagc agagtatttg aagatgagtt actttatttt atggaaaaag gaactgcaaa
    13081 aatatctgca aaagctactg ttaaaaaagt agaaaactat gttaaattaa cagaagatga
    13141 aatttctaaa attttaaatg aaaatcaacc gaaattaaat ttatctgaaa agcaaaaggt
    13201 tcgttggcat aaaaaatgtt tatgcttagt tgaatttgaa aatgttgaag aaataacacc
    13261 tcttgatttt gaccatcaag ggaatatgga cgattggctt attgttgaaa aaattgaaga
    13321 tgtagttatt ggcactagta taccttacaa ttacgagaaa tctaaatttt aaagtatcaa
    13381 taacataata ctatattact aaactttact taaataaaaa aagagtgtat ttaataatta
    13441 taaaaattat ttttatacac tcttttttaa taataatttt aaattactta ctttctaatc
    13501 tttcatatac atctataaat tcttctgcta attgttgaaa attcatagct gtcattaaat
    13561 ggtcttgtgc atgaataagt ataacactta tttcagtttt attaccatta gcttcatttt
    13621 gtatcaattc tgtttggaat ttatgagctt tatgaattga ttctttcgac ttttcaatac
    13681 attctctagc tttatctata ttcccgctct ttgcttcttt tattgcttca aatgctagcc
    13741 ctttcgcatc acctgcaaat ccaattattc caaaagatat ctctatcatc ttctcatcca
    13801 tatattcgac ctcctaaaca tctctatagc ttgatatgac taatccattc ttttctaaaa
    13861 attctttgac ttttttagat gtaagtatct tgtgctcttt cgttctatca atagcataag
    13921 atgtagaatt taaaatataa tcatctaaaa aagctgggtg agacataata tccactacat
    13981 catacttcat tatttcgtct atatttttta taaaatattc ttcacttaca ttttccttat
    14041 aaaaactatc tattaaagga actactttag aatactctaa attatattca aatccacctc
    14101 ttattggaag attatacttt attacaatct tctctattac tggctttaaa cttactaaag
    14161 tatggatatg atggtgactg tctaaatgag atatatctat tccaagccct tttactctat
    14221 ctatttgagc acaaagttct ctataaatct catcacaatc cattttttca ataatttcgt
    14281 ctgtaatcct cctaataaaa aaaccgtctt tatcaacaat agtttttaag ttagataata
    14341 atggtctacc acaacttaaa gtcatatgaa ctccacaatt taaagtttta ttttctttaa
    14401 gtaagcccac tccatgttct accccaggca tatttgccat catagaagta gacctaacta
    14461 taccattatt atgagctgaa attattccat agttcacagc ttcacaatat ccaaaatcat
    14521 ctgcatttat tataatttta gtcataccat aacctacttt ctatagacaa ctatctgtta
    14581 aatctaggaa gataatcttt atgagcttcc aacatttcgt ctaatatagt ttttgctaag
    14641 tcatcagatg gaatcaatgg gtttatacaa agtgccagaa gagcagattc ataatcgcca
    14701 tatactgctg ctttagctgc caatacttca aatgatttga tttggcttac taatccatgc
    14761 acagaatctg gtaaatatcc tatacttatt ggttttggac cattttttgt tattacacta
    14821 ctcacttcta ctgcttgttc atctttaaaa tctcttatag aaccattatt taatgtatta
    14881 actacttgga tatctttttt gtcattgtat atagaactta ttaaattaca agcagcatca
    14941 ctatagtaag cccctcctct tttttcaagt tgaggtggtt ttacatctaa tttttcatcc
    15001 ttatataatt caaataattg ttcttccaat tctttaacta cttgacctct agcttttccc
    15061 tctttaaatt ctttcaactc atcctcaagc atttctttag ttttatagta gtatctatgg
    15121 tatggacaag gtattgcacc caaagatttt acaaattcag cattaaaatc aattgccttt
    15181 atattttgca tacttatatc tgctttgaca aacttatcaa ttgcttcttt tgtaacatct
    15241 tctccatcta aagatacatt tagaccataa accatgtgat taagacctgc aaaatccata
    15301 cttattctgt cactttctac attaaatagt ttggcaacat cattttttaa atgaattggc
    15361 acattgcata gacctatata tcttctaaaa ttagtatatt tgaatacagc ctctgttatt
    15421 attcctgttg gatttgtaaa attcaccatc catgcatttg gacataattc ttccacatct
    15481 tttattatat caaatataac tggtattgtg cgaagggctt taaataaacc tccagcgcca
    15541 ttagtttcct gccccataac tccatgagat aaaggaatag tttcatcttt tattctcgca
    15601 tctaaaagac ctactctaag ttgtgttgtc acaaaatctg catctcttag agcttctctt
    15661 ctatctaaag taagatttat tttcatatct actccagctt tttttaccat cctcttagct
    15721 agatttccaa ctatctctaa tttttctttt ccagcttcta tatctacaag ccataattcc
    15781 tttacaggta actcttcata tctatttata aaaccctcta caagctcagg agtataactt
    15841 gaacctcctc caattgtaac tatttttagt ccatttttca tctcattata ccatccttac
    15901 ctataattta atatatttct acaaaaccct atatatttat ttaaaacccc atacatttat
    15961 ttaccgaaat aaaactacct agttgttaat tttcaattta ttagcttcca ttttttcaga
    16021 tgctatgaca aatggaatat atattaatat tgataatatt aaattaaata gtgctaaagc
    16081 tccaccagtc cagtcacctg tggccattgc tccacctaat ataggaggtg ttacccaagg
    16141 tattttaggt aaaattactg gtgctactaa tccaaaatct attgctaagt atgatacagc
    16201 actacatatt actggtgcaa gtacaaatgg aatcataaat ataggattta aaactattgg
    16261 aagtccgaat atcaatggtt ctcctatatt aaatattgca ggtggcccac ctaatactat
    16321 catttcttta cgtcttcttc cagctattaa taaagctaca attaatccca ataccattcc
    16381 agaaccacct aaatatacaa atgcatcaaa gaatgcacct gcaagtacat ggtatccttc
    16441 cgttgctcct gcttgagcca aggcagcatt ttctccacct aatttcataa gtatagtttc
    16501 tgtaaaagga agtgctatat ttgcaccatg aagtccaaca gtccaaagta tatgaattat
    16561 aaatgcaata agcattgtag aacctagact atcagctaca tttgatagtg gtgctcctaa
    16621 ataagtattt aaaatatctg ttaagaaact tccattagaa agcattttta taaatattcc
    16681 tatcacagta aatatcatta tagttaacat tcctggtatt aactttgcaa atgatcttga
    16741 aactgctggt ggaactccat ctggcatttt tatagttaat ttctttactt tagataatct
    16801 tacaaatatc tcagttgata ataatcctat taatatagaa gagaataaag ctgctgtacc
    16861 taagtaggtt tgttgtatca ttccccatcc ttcaactact gtagttcctc cctcaggaac
    16921 tattttacca atttgtggag ccattataaa aaagatagat aaagctataa gcccagattg
    16981 taacccatca ctctcataag attttgctag aaaataagca acacctatta ctaagaatac
    17041 tgccattgta cctattgctc cccaccaaag gtcgcctcca aatgtggtcc agttttctcc
    17101 aaatatactt gacatcaaat ttttgtatgc ttctaatggt aagttattta tcaatgttcc
    17161 caatgcacct accatagtta taggtatcat tactataaat gcatctctta ctgctactaa
    17221 gtgcctttga gctcctattt tagcagctac aggcactata tatttatcca taaatgacat
    17281 gaatttttcc attttacatc ctccagtaca actttatttt gaactattta actctacagc
    17341 tctatctaac acagcttttc cattacatct tccataatcc atcatattta taacttctac
    17401 tggaatatta tatggttttg ccatttcagt tgcttttcct aatacatatc taacttgagg
    17461 tcctaaaagt aagacatcac aattttctat ttcatttttt aagttcactt ctgatatagc
    17521 ccatattttt gcttctatac cgttttcctt tgctgcatcc tccatttttg taactaataa
    17581 actagttgac attcctgctg aacaagctaa cattattttt atcatttttt cccctccaaa
    17641 ttagattcat taaatatttt ttaagtcata agttttttaa actatacatt taaaatatgc
    17701 ttttctatta attttttctt aatgtatata tcttattttt aaatgtatat acatttaatt
    17761 cttgatatta ttatatccca aatgtatata catttcaaga aaactttttc attttattta
    17821 aattttattc gacaacagca ttcaaagtta atgtatatcc atagtgatat gtgtgtgagt
    17881 actcaaaaat ctcactattt tgtaacttac aaatttggct tatatataaa agtggctctg
    17941 gttcttctaa ttttagatta tccatacact cttcatttga tattacagct tgtacttctc
    18001 tctctgacat ggctatcttg tatccacaca cttcttcaac aaaattataa agagaatttt
    18061 cacaatgagt cttatttaaa tttggaaata acttaactgg catataagta atcatatatg
    18121 aaactcttgc atcattggct aatcttactc tttttatttc ccaaacattt tcttctaaac
    18181 ttatattcaa cttacttgat aaattttcat ctgcctgaac tatttcaaga gataccaata
    18241 tattagatac tttatagcct ttactttcca tctctttagt aaatccactt acagacgata
    18301 tattatcata tatattattt gcaataacta tactacctac acctctttgt ctagttatat
    18361 agccttcttt aactagatta ttaagtgcag accttacagt cattctcgtt acattaaact
    18421 cttcgctcaa ctgtttttca gaagggatta aatccccttc cttcaaactg tctgaattta
    18481 ttaactctct aacatgattt tctataactt tataaattgg ctctttcatc taattcactc
    18541 cttattaatt aaatctttta tataataaga taattttata tacaaacaaa gaaaatgtct
    18601 atacatttaa gataaattaa attttattca gacatcttgt acaactttcc gagttttaat
    18661 aaagatataa atagacttat cattaatatg ccttttaata tacttgataa ggttatactc
    18721 caccatacac catttagacc caatatttct ggtctagata taaagtaagc caatggtact
    18781 cttgcaccag tcagtatagt aattatagta gaaggtatat aggttctccc taatcctttg
    18841 aaagctcctg ttgttattat ttctaaacac ataaaaaatt gagaatagcc taatatttta
    18901 agataaattg ctcctttttc tattgcctca ctttcatcta taaaaataga aaatatactc
    18961 tctcctgcaa atactaatac caaagtagtt attatcccaa gtacagaaga tacagttata
    19021 gctattttac aacctttatt tattctagaa tacttcttgg caccataatt ttgtccaaca
    19081 aaactgccta atgcaactga tagtccttca gctgtcatcc aggaaattgc ctctatttgc
    19141 gagcctactt tttgtactgc aacagctaca ggtccccatg atgctactat tactcctaat
    19201 aacattgaga atactgtaaa cataccactt tgtattgcta taggtaatcc cagtttatac
    19261 agaactttat aatatttagt ctctatttct ttaaatagcc ttaatttaaa atattcttct
    19321 ttacttttaa ctattatata tataaaacat gtagtaacta ctatctgagc aaatacagtt
    19381 gcaatagcag ctcctgcaac accaagtttt ggagcaaatc cccatccaaa aattaatata
    19441 gggtcaagta taatattagt tattaatcct actgtattaa tacgaaaagg agttttacta
    19501 tttccaagtc cgttgaaaat tgcagtaaat actggattaa taaaataaaa taccatacct
    19561 agagctacta ttatcaaata ctgtctagac atggttataa cttctaagtc tcctaatcta
    19621 aaaaaaccta tcaattgttt attaagtact attaaagaca ttgtatatat tattgctagt
    19681 ataatattta tttctattgc tgacttcata tatgatttag tagtgcttat attatttgca
    19741 ccaatacttt gtgctacttt tatctctcca cctacttttg aaatcataat aaatgccatt
    19801 gcaagccatg gaaaaaatcc agctgttcca actgcagcta ctgctttact tccagcttta
    19861 ccaacccata tcatatctat catgttatac cccatttgta taaatgaagt ccccattatt
    19921 ggaagtgata gtttaagtaa tttttctgta attcttcctt ctgttaaatt aagtctgtct
    19981 ttcattttcc acctctttta tattaaattt atttgcttat atagctagat taatcatttt
    20041 atttgattga gttcactatc cttcaattta tattatcata tctcagaatt gcatctcaac
    20101 catacttatg tttaagataa agtattttac ctatctataa attaatactt atgaatataa
    20161 atctagccat ttcatcaata catattataa cccttaactt aacttcaatt aagtatacat
    20221 attgta
//
LOCUS       DAEPXF010000065        19961 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain HB27
            SAMN13376389-rid12479423.denovo.069, whole genome shotgun sequence.
ACCESSION   DAEPXF010000065 DAEPXF010000000
VERSION     DAEPXF010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13376389
            Sequence Read Archive: SRR10518432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 19961)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19961)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001069624.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001069624.1
            Genome Coverage       :: 315x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/15/2021 01:54:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,165
            CDSs (total)                      :: 4,076
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 89
            rRNAs                             :: 10, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 10 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 19 of 69
            Pseudo Genes (incomplete)         :: 44 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 10 of 69
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19961
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13376389-rid12479423.denovo.069"
                     /strain="HB27"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="China: Hebei"
                     /collection_date="2017"
     gene            180..1355
                     /locus_tag="KRL67_001977"
     CDS             180..1355
                     /locus_tag="KRL67_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HBH1522856.1"
                     /translation="MNLKQLSEKYEEYVIQMRREFHKYPELSFKEIRTTQRIREELQK
                     IGVPFVSIEPNIVIATIQGKNEGKTIAIRGDIDALPMQEDSDVEYKSEYDGIMHSCGH
                     DAHGAMLLGLAHMLNDIRKDLKGRVLLVFQAAEEVGGGHKEIIQYFKENGGPDRLIAT
                     HVWSDIEAGKISVEPGPRMAGGSGWRIDITGRGGHGSRPDQSIDPIKPACDIVLKISS
                     IPVNHVSVLEQCVVHACAIHGGTTIGNIIPNSAEIIGGYRYFTEESSKKVFDTIKQMS
                     EGIGQVYGVDVKVTHTGGIGPVINNEESVKMAKEVVSNIDGLELDSYEPICASDCYGE
                     ILKEYPGFYCYLGVGNKEKGIIYAQHHPKYNIDEDTLKLGCEFMAKYAVEFLNKNDN"
     gene            1389..2927
                     /locus_tag="KRL67_001978"
     CDS             1389..2927
                     /locus_tag="KRL67_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbgT family transporter"
                     /protein_id="HBH1522857.1"
                     /translation="MQSALKEDKKKSGGGFLGWIERVGNKMPHPLALFTYITLIVVAL
                     SAIASFLGFSAISPSTGKLIEVMNLISAKGLILFMQEFVKNFQNFPVLGVVLVMAVAT
                     GVCEKTGFFSTAIKMSLSKVKGNAVVFIIAFIGVFANQAGDAAFVLVPTLAGAIFYGL
                     GRNPLAGIFCGFASVGGGFATAVIPGGWDVTLTPITVSAAQTIQPAFDMTLLASYYAL
                     FVSAFIVATVSTIVTVKFIEPKLGKYTGKPEGMDDNQGFEVTKEQAKAAKLAGFTVLA
                     YVALLVALCIPANSFLRSPEGSLIFGAPLMSCLQFFIVILFFLPGIVYGMKVGKIKTV
                     KDLNDILCQSMATMAPFIVLAIVIGQFLTLFSVSNLAQVLAIKGGALLNSLPLPPQVI
                     ILLFLVLVAFINIFVISGSTKWMIFGPVFVPMLMQLNIHPAFTQFVYRLGDSVTNHLS
                     PLNAFFIILLATVQKYDKNAGMGTIFSAMIPYTIGYFVVLAVLVVVWMTIGIPVGIGG
                     QVWL"
     gene            2959..4161
                     /locus_tag="KRL67_001979"
     CDS             2959..4161
                     /locus_tag="KRL67_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HBH1522858.1"
                     /translation="METTEVIYNFDEVIDRSGTNCLKYDLKAQFMPSSPEDALPLWVA
                     DMDFACAPEIINAMHKRIDRKIFGYSSQQTDDYYEAVCGWFKRRFDWKIEKENIFFSP
                     GVVPALGFFTQILTKKGDGIIIEKPVYYPFEAKITNNERKVVNSPLKFEDGHYSMDYD
                     GFEELAKDENNKVFILCSPHNPVGRVWKKEELRKIVDICKKYDLWIISDEIHCDIIRK
                     GEKHIPLEVACPDYKDRIITCTAPSKSFNLAGMQLSNIVINNKELQEKWVFETDAKLA
                     IFLPNPFAIVATKVAYNNCEDWMNQVNEYIDENIKYVEDFVNEYMPKVKMIKSQGTYL
                     VWLDFRGYELDAKQLEDIMLNKAKVLLDEGYIFGEEGAGFERINVASPRSIIEECMNR
                     IRSAFEGL"
     gene            complement(4269..4868)
                     /locus_tag="KRL67_001980"
     CDS             complement(4269..4868)
                     /locus_tag="KRL67_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBH1522859.1"
                     /translation="MNDNIVEVKNINMNFYTKEGELEVLKDVNFNLKEGEILTLLGPS
                     GSGKSTILNILTNLLKPTSGDIKITGNIGYMFQKDNLLEWRNIMDNITIGLEIQGKKD
                     KKSLDRVESLLKTYGLWDFRSMYPKELSGGMRQRVALIRTLSVNPDILLLDEPFSALD
                     YQTRLLVCDDVYSIIKNENKSTILVTHDIGEAISIKVQL"
     gene            5038..6396
                     /locus_tag="KRL67_001981"
     CDS             5038..6396
                     /locus_tag="KRL67_001981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH1522860.1"
                     /translation="MSNIITNLIESIFTNPITILFLLAITVYSVFKIIENNRVYSFVS
                     SKLDEINRDYKTSEFYKKVRDDYYTYSKENPYADVNITSFIEEVVSDLKHNNLPLLEK
                     IRSIKNSSSISILLGVLGTFVGLSTMLLCVDTKDIINSLPSTISSMQTAFTTSIFGVV
                     FSLIIGYFTKIKDCEHVLIQIMLKSENLLTSEITHFKSERMDLKVEEVKNTIKQISKS
                     IEAIEKFDKISKDLNDFNDEFISGIEALKSLLEGSQSSIKTFDQSVRKLDKQFNILNI
                     KFVKLFDKYDNQDNINKEILFDIKESSKNIYNATESQFKIRDYIKNINAGFALYERSA
                     QDLLTKLMTHENKISQNQKILLDEKFTLDDSIKNLSSIIENFSNDLQVKLDMMFENSL
                     DIQDKLDVMFNNSFMNDEVPLDSEELFKDDVNNVFNPFSEEIYEIEDKEIKVIGEDEL
                     NE"
     gene            6389..7021
                     /locus_tag="KRL67_001982"
     CDS             6389..7021
                     /locus_tag="KRL67_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HBH1522861.1"
                     /translation="MNNKYRRTVNREFEKSSFWPTFTDLLSTVLMVVILILFSSESIS
                     GSVEQDLAKNVNASVEETFKKSGIPVKVDKSNGQVTFGERTMFDVDSDVLKPEAKEML
                     KMFVPKYIETIYKDYGDYISKIVIEGHTDDVGSYIYNLDLSQRRAYSVAKFIVGDEIG
                     DYKYKDKVTKHIIAIGRSKAELIKNSDNSVNRDASRRVELKYEININQNK"
     gene            7350..8498
                     /locus_tag="KRL67_001983"
     CDS             7350..8498
                     /locus_tag="KRL67_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HBH1522862.1"
                     /translation="MIERSIKNSKLKTLILLLIVSISIYLTGCATSDNGNNQSNNNDS
                     LSSSISSDKNVKIHFINTGNSDAILIQDGKTFTLIDGGDNDDEKLMVDYLNNQGVKDI
                     KYLIATHSHADHLGGLDSVVKNFNIENVFVSNGSAETKSYRDFINALANKDLSPSVPL
                     ENNKFYLEDSYFEVLNTNGGDSTNEQSLVLVYTNGNDKVLFTGDAEEGTEKEILPKLE
                     KVDLLKVAHHGSRSSSSQEFLDKINPEYAVLLVGKGNSYGHPHQETMNKLKKMGVKVH
                     RSDECSDIIFESTGDGVFTSCKDGSYNKGVREDGNSGKSNSNTSKNNSFKNKQNTSNS
                     EEIVYFTPKGKSYHSTKNCSGLSRSKKILSGTIAESKKNDPCDICYGK"
     gene            complement(8645..9280)
                     /gene="lexA"
                     /locus_tag="KRL67_001984"
     CDS             complement(8645..9280)
                     /gene="lexA"
                     /locus_tag="KRL67_001984"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HBH1522863.1"
                     /translation="MYLDLTEKQVLILEFIKSQITLKGYPPAVREICTAVGLRSTSTV
                     HSHLNKLEKLGYIRKDPTKPRAIEVLERSKVNDVSGANQEIIELPLVGQITAGEPILA
                     QQNIEEYIPFPASLVKGSNNFVLRVKGESMINAGILDEDYVVVDKKNTALNSQIVVAL
                     INGESATVKRFFKEGNLIRLQPENDFMEPIMLNDSEVEIVGIVTGVFRVIK"
     gene            complement(9384..10511)
                     /locus_tag="KRL67_001985"
     CDS             complement(9384..10511)
                     /locus_tag="KRL67_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HBH1522864.1"
                     /translation="MNNDKKIIKVHNKSDKPDNIVIKENELIEKCLLLENKLPAFMKD
                     YFIYLKGSVAVSTRLAYLEDINFFCSYLIETKELTNADCVKDITEDDFNTIKSRDINL
                     FLGDYCSRYYKNTEKNTLIFENNNRALARKKSSISTLFKFLYRNSQIDNNITDGFNPI
                     KLPKPQPDAIKRLEIDEVAKMLESVETGEGLTEKEKVYWRKTKLRDKAILALFVTYGL
                     RLNELRELNISSFNFSRGEFKIYRKRGKEVLMPINHTCEHVIKDYLQNERTRDELLND
                     EEKDALFLSLQNKRITAKAIRTLVKKYTSIPLDTTRDNGYSPHKLRATAATSLIQTGF
                     SIYDVQNLLDHDNVTTTQLYAAHKKNVKRDIVKNFEWIEDD"
     gene            complement(10833..11678)
                     /locus_tag="KRL67_001986"
     CDS             complement(10833..11678)
                     /locus_tag="KRL67_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH1522865.1"
                     /translation="MIYIVEILKISIINIFYFIGIFIVFGLIFSITENLNNKFIFSSF
                     GKTGIIVTGAIGTFVHELSHLIMCLIFMHKINSVKFFRPIESKNDGILGYVSHSYKKD
                     NLYQSIGNFFIGIAPIIGGTIMIILIFKILLPDSYMKVTQNIDLKLYVSMINNLNING
                     FIHIILGDISNFIRIMLLTPSVYSLKYFIFMFLMYSISTHMSLSSADLKNSLNGLSFI
                     VIVIFVISSITYLFGFDNLNISSLIVKYNIFISFFMTIGFIFSIVTLIISYILSLVSP
                     FKNFS"
     gene            complement(11708..12079)
                     /locus_tag="KRL67_001987"
     CDS             complement(11708..12079)
                     /locus_tag="KRL67_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HBH1522866.1"
                     /translation="MKLKLLVEEYAVCRLNNDSKIPTWIDTEKFYSITRTDDELSVVC
                     LNNNIPSDVKSEKEWRILKILGPLDFSLVGILSKLSSLLADNKISIFAISTYDTDYIL
                     VKEKDIENACKILNCNGYEVE"
     gene            complement(12232..12492)
                     /locus_tag="KRL67_001988"
     CDS             complement(12232..12492)
                     /locus_tag="KRL67_001988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003866776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein S"
                     /protein_id="HBH1522867.1"
                     /translation="MEVLKVSSKSNPNSVAGALAGVLRERGVAEIQAIGAGALNQAIK
                     SIAIARGFVAPSGMDLVCIPAFTDIEIEGDKKTAIKLIIEPR"
     gene            complement(12681..13625)
                     /locus_tag="KRL67_001989"
     CDS             complement(12681..13625)
                     /locus_tag="KRL67_001989"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit alpha"
                     /protein_id="HBH1522868.1"
                     /translation="MIENNSEKIKVLENDIKQLITISKANNIDLSDKINSLNEKLEKL
                     KEDAFSHLSAYEKVTLSRDIKRPTTLEYIEHICSNFLELHGDRLFKDDPSIVGGIGQI
                     GKFNVTIVGHQKGRDTKENIKRNFGMPHPEGYRKALRLMKQAEKFNRPIITFIDTSGA
                     FCGLEAEERGQGEAIARNLLEMSKLSVPVITFVIGEGGSGGALGIGVGNDVCMLEHSV
                     YSVISPEGLSSILFKDSSKAKEACDVMKLTSNDLYDLKIIDKIIKEPLGGAQKDVEAV
                     SKEIKAYILERLNHYKDMDKEEIIAQRYNKFRNIGKCL"
     gene            complement(13646..14500)
                     /locus_tag="KRL67_001990"
     CDS             complement(13646..14500)
                     /locus_tag="KRL67_001990"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit beta"
                     /protein_id="HBH1522869.1"
                     /translation="MIKKFLSSKESKYVTISLDDNFKKNSVDDKFWTYCKGCDSHVFR
                     KDIEENSFVCPKCSRHYGLRARKRINLLIDKGTFMEFNSDVEFQNPLNFPKYKEKVDS
                     YKEKTKESEAVVTGYGRINGIKTVICVMNPDFMMGSMGSIVGEKITYSIEYAAENNLP
                     IIICSASGGARMQEGMVSLMQMAKTSQALSKLEEKSLPYISVLTDPTTGGVTASFAML
                     GDIIISEPNTLIGFAGPRVIEQTINQKLPEGFQTSEFLLEKGFIDMIVDRRKMKEVLY
                     QILAMHKK"
     gene            complement(14515..15876)
                     /gene="accC"
                     /locus_tag="KRL67_001991"
     CDS             complement(14515..15876)
                     /gene="accC"
                     /locus_tag="KRL67_001991"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HBH1522870.1"
                     /translation="MIKKILVANRGDIAVRIIRTCKELGIKTVAIYSEIDKDCFHRYI
                     ADESICIGPNNISKSYNNIENIIYLALKLKCDAIHPGFGFLSENPEFAKQCEENNIIF
                     IGPTREQMILMGDKSRARETMMELNIPVVPGSESVLKTKEEALEVAREIGYPVMIKAS
                     SGGGGKGMRIVRKEEELFSNFDMASSEALAAFSNSDLYMEKFIENPRHIEVQVFGDKH
                     SNAIHLGDRDCSMQRRNQKVIEESLSPYLSDEERQKLHKIAVDIVKGVGYIGAGTIEF
                     IVDKDKNFYFIEMNTRIQVEHPVTEMVTNLDLIKLQISIANGDKIPFKQEDITFRGHA
                     IECRINAEDSSKNFAPSPGKIESLNLPGGFGVRFDTFVYAGYTIPPLYDSMIGKLICW
                     AETREECISRIYRALDEIIVEGINTNVEFQKALVTSEEFREDTHHTKFIEDVFMKKEF
                     ATL"
     gene            complement(15892..16344)
                     /gene="accB"
                     /locus_tag="KRL67_001992"
     CDS             complement(15892..16344)
                     /gene="accB"
                     /locus_tag="KRL67_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HBH1522871.1"
                     /translation="MNINEIKELLKAIDSTNLEYVKLESSDLRLEVSKKAQSTSSPVL
                     SVQQESVVDLSLEKPVVNDTPVTSNENLSVVVAPLMGTFYDSPSPDADSFVKVGDVVE
                     EGDTLCILEAMKLMNEITSEIKGEIIEVLVSNEELVEYNQPLFKIKPL"
     gene            complement(16614..17060)
                     /locus_tag="KRL67_001993"
     CDS             complement(16614..17060)
                     /locus_tag="KRL67_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH1522872.1"
                     /translation="MESKFFNWYTQSLGGTLGIISCICAYLNGYMFVYTNSASKYFES
                     IGFLGIISSYILLPLCMLTLIFGVIYSYTPETSDKSIFNIPFFTLNKRLILTTVVFGF
                     IGARVYFIIPAILITFNLYSDILIAKLKCILCKNKENDANLKESEV"
     gene            17345..17623
                     /locus_tag="KRL67_001994"
     CDS             17345..17623
                     /locus_tag="KRL67_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH1522873.1"
                     /translation="MVDTLEFGLKILFFILSIIWMGKIMILRTDKQIVINPLLIGISA
                     VLVMLHTSQSNIEFFGLDVQYIRIVLYIIYSLIILIGIWATNRRNGIF"
     gene            complement(17752..18498)
                     /locus_tag="KRL67_001995"
     CDS             complement(17752..18498)
                     /locus_tag="KRL67_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH1522874.1"
                     /translation="MWEGTMNLANLIPFIISAAALSPINSNDKVLSENQLKDIFPDAN
                     LRAVVKRYINPDEMTISNIKALDGEFYATGESISNLKGISYLENVDNFIFWNNNIKEV
                     PKEALSLKDMDSINLANNYLIDDGVVNSLSNNGVDVNCDLNFIDTKDNQYKLDSKYHN
                     VDIAKGENIDLRKIIMKKIDSYYKYWEVTDNLPKDLDFIVSVSDKSVLSCEDMIIKGN
                     KTGQAVVKVILSDKNDLNTSQEVLITVNVK"
     gene            complement(18627..19271)
                     /gene="trmB"
                     /locus_tag="KRL67_001996"
     CDS             complement(18627..19271)
                     /gene="trmB"
                     /locus_tag="KRL67_001996"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HBH1522875.1"
                     /translation="MRRRRKKGADEKLLSYTKYVLRDDIDKLKGKWNLKFRNDNPIHV
                     EFGTGKGKFITTLAKQNPDINYIAMELKEEVLLKAVEKADASNLNNILFLWGDVSNIL
                     DYFEAKELSRIYINFCDPWPKNRWSKRRLTHSGFLEMYNRVLEDDGEIHFKTDNEKLF
                     EFSLNEIAANNWLLKNISLDLGNSEYENNVTTEYEDKFMSQGMRIFRCEAKKRN"
     gene            complement(19409..19729)
                     /locus_tag="KRL67_001997"
     CDS             complement(19409..19729)
                     /locus_tag="KRL67_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein HesB"
                     /protein_id="HBH1522876.1"
                     /translation="MKITLPETAIDTLKSILKDNQDKPNNIRVYFAGVGCGGPSFGLA
                     LDEKKEDDLTYEVGELQFVMSSDEYSQYGDIIIEDTGFGFRVIPENMKDQGGGGCSGC
                     SGCH"
BASE COUNT         7123 a         2905 c         2708 g         7225 t
ORIGIN      
        1 aatataaata gaaaaatgct ataacatgtt gacttaatgc ggaaaaaaat taaaaagatt
       61 aagataaaca aaaaggaaaa cgttttatat tgacatagag aaaaagtagt gatactattt
      121 aaatatagtt tagcagctaa actatattta aattataata actattttag ggggatgtta
      181 tgaatttaaa acaattaagt gaaaaatacg aagaatatgt aattcaaatg agaagagaat
      241 ttcataagta tccagaatta tcttttaaag aaataagaac aacccaaaga ataagggaag
      301 aattacaaaa aataggggtt ccttttgtat ctattgaacc aaatatcgta atagctacta
      361 tacaaggcaa aaacgaaggt aaaacaattg caattagagg agatatagat gcgcttccaa
      421 tgcaagaaga ttcagatgtt gaatacaaat cagaatatga tggaataatg cattcttgtg
      481 gacatgatgc tcatggagct atgttactag gacttgcaca tatgctaaat gatataagaa
      541 aagatttaaa aggtagagta ttattagttt ttcaagcagc tgaagaagtt ggtggcggac
      601 acaaggagat aatacagtat tttaaggaaa atggaggacc agatagatta atagctactc
      661 atgtttggtc cgatattgaa gctggtaaaa tttctgttga accaggacct cgtatggctg
      721 gtgggtctgg ttggagaata gacataacag gtagaggagg tcatggttct agaccagatc
      781 aaagtataga cccgataaaa ccggcttgcg atatagtttt aaaaatatct tctataccag
      841 ttaatcatgt aagtgtatta gaacaatgcg ttgtacatgc atgtgcaata catggaggaa
      901 ctacaatagg aaatattatt ccaaacagtg cagagataat aggcggatac agatatttta
      961 ctgaagagtc aagtaaaaaa gtgtttgata ctataaagca aatgagtgag ggtattggac
     1021 aagtttacgg agtagatgtg aaggttactc atactggagg cataggacct gttataaata
     1081 atgaagaatc agttaagatg gctaaagaag tagtttctaa tatagatgga ttagaattag
     1141 attcttatga gccaatatgt gcatcagatt gttatggtga aatattaaaa gagtatcctg
     1201 gtttttattg ctacctaggg gtaggtaaca aagaaaaagg aataatatat gcacaacatc
     1261 atcctaagta caatatagat gaagatacat taaagttagg ttgcgaattt atggctaaat
     1321 atgcagttga atttttaaat aaaaatgata actaaaataa atctaatttt ataaatgggg
     1381 ggataaaaat gcaaagtgca ttaaaggaag ataagaaaaa aagcggtggt ggatttttag
     1441 gatggattga aagagttggc aataaaatgc cacatccact agcattattt acatatatta
     1501 cattgatagt agtagcatta tctgcaatag caagcttttt aggtttctca gcaataagtc
     1561 cttctacagg caagttaata gaagttatga acttaataag tgcaaaaggt ttaatactat
     1621 ttatgcaaga atttgttaag aacttccaaa acttccctgt acttggagtc gttttagtta
     1681 tggcagttgc aacaggggtt tgtgaaaaga caggattttt ctcaactgct ataaaaatga
     1741 gtttatcaaa agttaaggga aatgcagttg tatttataat agcttttata ggagtatttg
     1801 ctaatcaggc tggtgatgct gctttcgtat tagtaccaac tcttgcaggg gcaatatttt
     1861 atggattagg tagaaatcca ttagctggta tattttgtgg atttgcatca gttggtggtg
     1921 gatttgcaac agccgtaatt cctgggggat gggatgttac actaacacca ataactgtat
     1981 cagcagctca aactattcaa cctgcatttg atatgacatt attagctagt tattatgcac
     2041 tatttgtatc tgcatttata gtagctacag tatcaacaat agtaactgtt aaatttatag
     2101 aacctaaatt aggtaaatat actggtaaac cagaaggtat ggatgataat caaggctttg
     2161 aggtcacaaa agaacaagca aaagcagcaa agttagcagg ttttacagtg ttagcttatg
     2221 tagcattatt agttgcttta tgtataccag ccaatagttt tttaagaagt ccagaaggtt
     2281 ctttaatatt tggagcacca cttatgtctt gcttacagtt ttttatagta atactattct
     2341 tcttaccagg gattgtgtat ggaatgaagg ttggcaaaat caagactgta aaagacttaa
     2401 atgatatatt atgtcaatca atggcaacaa tggcaccgtt tattgtactt gcaatagtta
     2461 taggtcagtt cttaacacta ttttcagtat ctaacctagc acaggtacta gctataaaag
     2521 gaggagcatt acttaactca ttgccattac caccgcaagt tataatactg ctattcttag
     2581 tactagtagc atttataaat atatttgtta taagtggaag tacaaaatgg atgatatttg
     2641 gaccagtatt tgttccaatg ctaatgcaat taaacataca tccagcattt actcagtttg
     2701 tatatagatt aggtgattct gtaactaatc atttatcacc attaaatgca ttctttataa
     2761 tacttttagc aacagttcaa aaatacgata aaaatgcagg tatggggact atattctcag
     2821 ctatgatacc ttacactata ggatactttg tagtacttgc agttttagta gtagtttgga
     2881 tgactatagg tataccagta ggtataggtg gacaagtttg gttataaaac atatttaata
     2941 attttgggag gaaagaaaat ggaaacaact gaagtaatat acaattttga tgaagtaata
     3001 gatagaagtg gaacaaactg tcttaaatat gaccttaagg ctcaatttat gccgagctct
     3061 ccagaagatg cactaccact atgggtagca gatatggatt ttgcatgtgc tcctgagata
     3121 attaatgcaa tgcataaaag aatagacagg aaaatatttg gatatagctc tcaacaaact
     3181 gatgattatt atgaagctgt atgtggatgg tttaagagaa gatttgattg gaaaatagaa
     3241 aaagagaata tattctttag cccaggagtt gtacctgcgt taggattttt cacacaaata
     3301 ctaacaaaaa agggtgatgg tataataata gaaaagcctg tatactatcc ttttgaagct
     3361 aagataacta ataatgaaag aaaggtagtc aatagtcctt taaagtttga agatggtcat
     3421 tattctatgg attatgatgg atttgaagaa ttagctaaag atgaaaataa taaggtgttt
     3481 atattatgta gtcctcataa cccagttggt agagtatgga aaaaagaaga gcttagaaaa
     3541 atagtggata tatgcaagaa atatgattta tggataatat ctgatgaaat acactgtgat
     3601 attattcgta aaggtgaaaa acacataccc ttagaagtag catgccctga ttataaggat
     3661 cgaataataa cttgtacagc tccaagtaag agttttaact tagcgggtat gcaattatca
     3721 aatatagtaa tcaacaacaa agaattacaa gaaaaatggg tgtttgaaac agatgctaag
     3781 ttagctatat tcttacctaa tccatttgca atagttgcta ctaaggttgc ttataataat
     3841 tgtgaagatt ggatgaatca agtaaatgaa tatatagatg aaaatattaa atatgtagaa
     3901 gattttgtta atgagtatat gcctaaagta aagatgataa aatctcaagg aacttactta
     3961 gtttggttag actttagagg gtatgaatta gatgcaaaac aacttgaaga tataatgctt
     4021 aacaaggcga aggtattgct agatgaagga tacatattcg gagaagaagg agcaggattt
     4081 gaaagaataa atgttgcttc acctagatca attatagaag aatgtatgaa tagaataaga
     4141 agtgcatttg aaggattata atagtaatat ctaaataata tatttgtgta tttaaaaaaa
     4201 agactatgct agaaaaatat ttcaaagcat agtctttgaa agttttatga tgacaatata
     4261 ctcattttct aaagttgcac ttttatagat atagcttcac caatatcatg tgtaaccaaa
     4321 atagtagact tattctcatt cttaataata ctataaacat catcacaaac taataatcta
     4381 gtctgataat caagagctga aaaaggttca tctaaaagta gaatatctgg atttacagaa
     4441 agtgttctaa taagcgcaac tctttgtctc atacctccag ataattcttt tggatacata
     4501 cttctaaaat cccataatcc ataagtcttt aaaaggcttt caactctatc taaagatttt
     4561 ttatcttttt tgccttgaat ttccaatcca atagttatat tatccattat atttctccat
     4621 tcaagcaaat tatctttttg aaacatatat ccaatatttc cagtaatctt tatatcacca
     4681 ctagtcggtt ttaaaagatt agtaagaata tttaatattg tagatttacc acttccagaa
     4741 ggaccaagca aagttaatat ttctccttct ttcaagttaa aattgacatc ttttaatact
     4801 tcaagctcgc cttccttagt gtagaagttc atatttatat ttttaacttc tactatgttg
     4861 tcattcatta tgccaccccc tataactttt agctcgtata tattatgtta ttaacaatat
     4921 gacctatttg taccattttt cataaatata atataaatat tttgtttatt tacgtatttt
     4981 cttagtaact tgtacgtata aattatagat aaataaatta taaggtaggg gataaatttg
     5041 agtaacataa taacgaattt aatagagtca atatttacta atccaataac tatattgttt
     5101 ttacttgcaa taactgtgta ttctgttttt aaaataatag aaaataacag agtatattca
     5161 tttgtaagtt caaaattaga cgaaatcaat agagactata agacaagtga gttttataaa
     5221 aaggtaaggg atgactacta tacatatagt aaagaaaatc cttatgcaga cgtaaatata
     5281 acttcattta tagaggaagt tgtatctgat ttaaagcata ataatttgcc attgttagaa
     5341 aaaattagaa gcataaaaaa ttcatcttca ataagtatat tacttggggt tttaggtacg
     5401 tttgttggtc tttctacaat gcttttatgt gttgatacta aagacattat aaattcactt
     5461 ccttcaacta ttagtagtat gcaaactgca tttacaacta gtatatttgg agttgtattt
     5521 tctctaatta taggttattt tactaagata aaagattgtg aacatgtttt gatacaaata
     5581 atgttaaaat ctgaaaattt actaacatct gaaataactc attttaagtc agaaagaatg
     5641 gatttaaaag tagaagaggt aaaaaataca ataaaacaaa taagtaagtc tatagaagct
     5701 attgaaaaat ttgataaaat atctaaagat ttaaatgact ttaatgatga gtttatcagc
     5761 ggtatagaag ctttaaaaag ccttttagag ggttctcaaa gttctattaa gacatttgac
     5821 cagagcgtaa gaaaattaga taaacagttt aatattttaa atataaaatt tgtaaagctt
     5881 tttgataaat atgataatca agataatata aataaagaga ttttattcga tataaaagaa
     5941 tcttcaaaaa atatatacaa tgccactgaa agccaattca aaattagaga ttatataaaa
     6001 aatataaatg caggatttgc tttatatgaa agaagtgctc aagatttatt aactaaatta
     6061 atgacccatg aaaataaaat atctcaaaat caaaaaatcc ttttagatga aaagtttacc
     6121 ttagatgact caataaaaaa tctctcaagt attattgaaa acttttctaa tgacctacaa
     6181 gttaaactgg atatgatgtt tgaaaattct ttagatattc aagataagtt agatgttatg
     6241 tttaataact cttttatgaa tgatgaagtt cctcttgaca gtgaagaatt atttaaggat
     6301 gatgttaata atgtatttaa tccttttagt gaagaaattt atgagataga ggataaagaa
     6361 ataaaggtca taggtgagga tgagttaaat gaataataaa tatagaagaa ctgttaacag
     6421 agaatttgaa aagagtagct tttggccaac atttacagat ttattatcta ctgtattgat
     6481 ggttgtgatt cttatcttat ttagctcaga aagtatttca ggttctgttg aacaagattt
     6541 agctaagaat gtcaatgctt ctgttgaaga aacttttaaa aaaagcggaa tacctgtgaa
     6601 ggtagataaa tctaatgggc aagtaacttt tggtgaaaga accatgtttg atgtagatag
     6661 tgatgtttta aaaccagagg ctaaagaaat gctaaaaatg tttgttccaa agtacataga
     6721 gactatttat aaagattatg gtgactatat atccaaaata gttatagaag gtcatacaga
     6781 tgatgttgga agttatatat acaacttaga tttatcgcaa agaagagcat atagcgttgc
     6841 taaatttata gtgggagatg aaataggtga ctataaatac aaagataaag tgactaagca
     6901 tattattgca ataggaaggt caaaagctga actcatcaaa aatagtgaca atagcgtgaa
     6961 tagagatgca tctagaagag tagaactaaa atacgaaata aatattaatc aaaataaata
     7021 attaaatttt tataaaaaaa aagaaggcag ttggtgtgag ctttaagcct tctttaagga
     7081 aaaagtttat ttatttttgt tgatacttaa tatattatag tttttagaag cagtttgcaa
     7141 tatattaaac gtaaatggtc gtttaaaatg cataactgga tactactgat gtaaaatatt
     7201 tcattttata taatataaaa attatacagt gtcaattttc tatgttattg tactataatc
     7261 tattatttaa aatattatgt aaaaaaatat cataaaaacg catatcatgt tataattact
     7321 agagtattat gaatatggag gcgtagtttg tgatagaaag aagtataaaa aattctaaac
     7381 ttaagacttt gatactttta ttaatagtat ctatatcgat ttatttaact ggttgtgcta
     7441 catcagataa tggaaataat caatcaaata ataatgattc tttgtctagt agtatatcaa
     7501 gtgataagaa tgtaaaaatt cattttatta acactggaaa cagtgatgca atattgattc
     7561 aagatggtaa aacttttaca ttgatagatg ggggagataa tgatgatgag aaattaatgg
     7621 tagattacct aaataatcaa ggtgtaaaag atattaaata tcttatagct acccattctc
     7681 atgcagacca tttgggtgga cttgattcag ttgtcaaaaa cttcaatata gaaaatgttt
     7741 ttgtatcgaa tggttcagca gaaacaaaga gttataggga ctttataaat gctttagcaa
     7801 ataaagattt atctccaagt gtaccacttg aaaataataa gttctattta gaagattctt
     7861 attttgaagt tttaaataca aatggaggag atagtactaa tgaacaatct ctagtccttg
     7921 tatatactaa tgggaatgat aaagttttat ttacaggtga tgctgaagaa ggtaccgaaa
     7981 aagaaatact acctaagctt gagaaagtag accttttaaa agtagctcat catggttctc
     8041 gtagtagttc atctcaagag tttttggata aaataaatcc tgaatatgcg gttcttttag
     8101 taggaaaggg taattcatat ggtcatccac atcaggagac aatgaataaa ttaaaaaaaa
     8161 tgggagtaaa agtacataga agtgatgagt gttcagatat aatatttgaa tcaacaggtg
     8221 atggcgtatt tacaagttgt aaagatggaa gttataataa aggcgttaga gaagatggaa
     8281 attctggaaa atcaaacagt aatacaagta aaaataattc ttttaaaaat aaacaaaata
     8341 catccaactc agaagaaata gtgtatttta ctccaaaagg taagagttat cactctacaa
     8401 aaaattgtag tgggctttca agaagtaaaa aaatattatc aggcacaatt gcagaaagta
     8461 agaaaaatga cccatgtgat atatgttatg gaaaataaca aaaaagcaac caatttatct
     8521 ataaagcttc taaactttaa atcatttaaa aaataaaata aatgcataaa aaaataagct
     8581 ataatcaata gataacacat tttatctata cgaatagctt atttttttaa gtatttttta
     8641 atagctattt aataactcta aatacaccag ttacaatacc tacaatttca acttctgagt
     8701 cattgagcat aattggttcc ataaaatcat tttctggctg aagtcttatt aaatttcctt
     8761 ctttaaaaaa cctcttaact gtagcagatt caccatttat aagtgcaact actatctgag
     8821 aatttaaggc tgtatttttt ttgtctacta caacgtaatc ttcatctaaa attcctgcat
     8881 taatcatact ttcgcctttt actcttaata caaaattgtt actgcccttt actaaactgg
     8941 cagggaatgg aatgtattcc tctatgtttt gttgagctaa aatgggttct ccagctgtta
     9001 tttgacctac taaaggaagc tctattattt cttgattagc tccagaaaca tcatttactt
     9061 tactacgttc taaaacttca atagctcttg gtttagtagg gtcctttctt atgtatccca
     9121 gtttttcaag tttatttaga tgagagtgca cagttgaagt agatcttaac cctacagcag
     9181 tacatatttc tcttacagca ggtggataac cttttaatgt gatttgagac tttataaatt
     9241 ccaatatcaa gacttgcttt tcagttagat ctaaatacat aacatcactc atttccaaac
     9301 atattttgtt tttatttcca tacactttat atttttaatt ataacataat ccatttatat
     9361 ctgcaaacat ttgttcctga tttttaatcg tcctcaatcc attcaaaatt cttgactata
     9421 tcacgtttta catttttctt atgagctgca taaagctgag tagttgtgac attatcatgg
     9481 tcaagtaagt tttgaacatc atatatagaa aatcctgttt gaattagaga agttgcagcc
     9541 gttgccctta atttatgggg actatatcca ttgtctctag tagtatcaag tggaatagat
     9601 gtatattttt ttaccagtgt tcttatagcc tttgctgtaa ttctcttatt ttgaagagac
     9661 aaaaataaag catctttttc ttcatcattt aatagttcat ctcttgttct ttcgttttgc
     9721 aaataatctt ttattacatg ctcacaagta tgatttatag gcattaaaac ttctttaccg
     9781 cgttttctat atattttaaa ttcacctctt gaaaaattaa aagaggagat attgagttct
     9841 ctaagctcat ttaatctaag cccatatgta acaaacaatg ctagtatagc tttatcacgt
     9901 aacttagtct ttctccaata taccttttct ttctctgtaa gcccttcacc tgtttctact
     9961 gattcaagca tcttagcaac ttcatctatt tcaagacgct taatagcatc tggctgaggt
    10021 ttaggaagtt ttattggatt gaatccatca gttatattat tatctatctg agagtttcta
    10081 tataagaatt taaatagagt agagatggat gatttttttc ttgctaaagc tctattgtta
    10141 ttttcaaaaa tcaatgtatt tttttctgta tttttataat atctactaca ataatcacct
    10201 aagaataaat ttatatctct tgattttatt gtattaaaat catcttctgt aatatctttt
    10261 acacaatctg catttgttaa ttctttggtt tcaatcaaat atgaacagaa aaaattaata
    10321 tcttctaaat aagcaagtcg tgtagaaact gcaacagaac cttttagata tataaaataa
    10381 tccttcataa aagcaggaag tttattttcc aaaagtagac atttttctat gagttcattt
    10441 tctttgatga caatattatc aggtttatct gatttattat gtacttttat aattttctta
    10501 tcattattca aaattaaacc tcctaaaact aaaatataaa tcttttatat tattttttga
    10561 tatttatacc aagtgagtat aaatatttaa tttaatcatt ttttgtattt ttaagagatt
    10621 agcagtgctt tagatgaaat cgccactaac tattccctaa atccacattt aaagactagt
    10681 taaatttaag tactcttttt ttatttctca atatatttat agaaatattt taaaatatat
    10741 tgagaaattt tttatgctct actaaaatct tttttatgta atatttttat atgtttctaa
    10801 caaaaccaaa tattagtaca atatgtattt atttaactaa agtttttaaa tgggctgact
    10861 aagcttaaaa tataggatat tatgagagtt actattgaaa aaataaaccc tattgtcata
    10921 aagaaactta taaatatatt atacttaact attaaactgg atatattcaa gttatcaaat
    10981 ccaaataagt atgtaattga acttattaca aaaataacta taactataaa gctcagacca
    11041 tttaaactat tttttaaatc agcactacta agtgacatat gtgtagatat agaatacatt
    11101 aaaaacataa atataaaata cttcaatgaa tatacactag gtgttaacaa cattattctt
    11161 ataaaattgg aaatatcacc taaaataatg tgtataaacc catttatatt taaattatta
    11221 atcatactta catataactt taaatctata ttttgagtca ctttcatata actatctggc
    11281 agtaatatct taaaaattaa aataatcatt attgttccac ctataatagg tgcaattcct
    11341 ataaagaaat ttcctatact ttgataaaga ttatcttttt tatagctatg acttacatat
    11401 cctagtatac catcattttt actttctatt ggtctgaaaa acttaactga atttatttta
    11461 tgcatgaaaa ttaaacacat tatcaaatga ctcaattcat gaacaaatgt tcctatagct
    11521 ccagttacaa taattccagt ttttccaaaa gatgaaaaga taaatttatt atttagattt
    11581 tctgtaatgc taaaaatcaa tccaaataca ataaatattc ctatgaaata aaaaatattt
    11641 attatactta tttttaaaat ttcgactata taaatcataa gttaaagcct catgtaaaag
    11701 tatttaacta ctctacttca tatccattac agtttaaaat tttacaagca ttttctatat
    11761 ctttttcttt tactaagata tagtctgtat catatgtaga aattgcaaat atgcttattt
    11821 tgttgtctgc taacaagctg cttaacttag ataaaattcc tacaagtgaa aagtctaaag
    11881 gacctaatat cttaagtatt ctccactcct tttctgactt aacatcagaa ggaatattat
    11941 tatttaaaca tacaacactt aattcatcat cagttctagt tatagagtaa aacttctctg
    12001 tatctatcca tgttggtatt ttactatcat tatttaatct acatactgca tactcttcta
    12061 ccaataattt taatttcata atgtatattt cctccaatct tatttgttta cttaagatta
    12121 tacattaaaa attacataaa ataaaacata taaagtaaaa aaggttcact atagtatcct
    12181 atttctatag ccaaccttta aaacttaatt aaattctaat aaagagatat attatctagg
    12241 ttcaattata agtttaatgg ctgttttctt atcaccttcg atttctatat cagtaaatgc
    12301 gggaatgcaa actaaatcca ttccactagg tgcaacaaaa cctcttgcta ttgcaattga
    12361 ttttatcgct tgatttagag ctccagctcc tatagcttgt atttctgcaa cgcctcgttc
    12421 tcttagtact ccagccaagg ctccagctac agaattagga ttagattttg atgaaacttt
    12481 tagtacctcc atttcaacat ctccttgtta atttgttaaa gtatccatta cctaaagtac
    12541 taaatcataa ttgccattta ctaattacaa tcatcattta aagtatactt ttataaaaat
    12601 atacttctat aatagtttta agaaaacctt tatttttttt aaccatttta ttatgaaaaa
    12661 actacaaatt tctttatatt ctataagcat tttccaatat ttctaaattt attatatctt
    12721 tgagctataa tttcttcttt atccatatct ttataatgat ttaatctttc taaaatataa
    12781 gcttttattt cttttgaaac agcttctaca tctttttgtg ctcctccaag aggttctttt
    12841 ataatcttat ctattatttt taaatcatat aaatcattgc ttgtaagctt cataacatca
    12901 caagcctctt tagctttaga tgaatcttta aatagaatac tagaaagccc ttctggagaa
    12961 atcacagaat aaactgaatg ttctaacata caaacatcat tgcccacacc aatacctaaa
    13021 gctcctccac taccaccttc gccaatcaca aaagtaatta ctggaacaga aagtttactc
    13081 atttcaagta agtttcttgc aatagcttct ccttgtcctc tttcctctgc ttctagaccg
    13141 caaaaagctc ctgatgtatc aataaatgtt attataggtc tattaaattt ttcagcttgt
    13201 ttcataagtc ttaatgcttt tctgtatccc tctggatgag gcattccaaa atttcttttt
    13261 atattttctt ttgtatctct tcctttttgg tgtccaacta tagtaacatt aaactttcct
    13321 atctgaccaa tccctccaac tatagaaggg tcatctttaa aaagcctatc tccatgaagt
    13381 tctaaaaaat tagaacaaat atgctctatg tattctaatg tcgtaggtct ctttatatct
    13441 ctacttagtg ttaccttttc atatgctgat agatgtgaaa aagcatcttc cttaagcttt
    13501 tccaatttct cattaagtga atttatttta tcacttaaat ctatattatt tgccttagaa
    13561 atagttatta attgtttaat atcattttct aagactttta ttttttcact attattttca
    13621 atcaaaacag ttcctccttt atgccctatt tcttgtgcat agcaagaatt tgatataata
    13681 cttccttcat ttttcttcta tctactatca tgtcaataaa acctttttca agtaaaaact
    13741 cagaagtttg aaatccttca ggaagttttt gatttattgt ttgctctatt actcttggac
    13801 ctgcaaaacc tatcaacgta tttggctcag agattataat atcaccaagc atagcaaaac
    13861 tagcagttac tcctccagta gtagggtcag ttagaactga tatatatggc aatgattttt
    13921 cttcaagttt agaaagtgct tgagaagtct ttgccatctg cataagagat accatacctt
    13981 cttgcattct agctcctcct gaagcagaac aaataattat aggtaaatta ttttcagcag
    14041 catactctat tgaataagtt attttttccc caacaataga acccatactt cccatcataa
    14101 aatcaggatt cattacacaa ataaccgttt taattccatt tattcttcca taacctgtga
    14161 caacagcttc tgactctttt gttttttcct tataagagtc tactttttct ttgtatttag
    14221 gaaaatttag tggattttga aattctacat cagaattaaa ctccataaaa gttcctttat
    14281 caatcaataa atttattctt tttcttgctc ttaagccata atgtcttgaa cattttggac
    14341 atacaaatga attttcttct atgtcctttc taaatacatg actatcacaa cctttgcaat
    14401 aggtccaaaa tttatcatct acactattct ttttaaagtt gtcatctaaa gaaatcgtaa
    14461 catacttaga ttcttttgat gataaaaatt tttttatcat aatctattta ctccttataa
    14521 tgtagcaaat tctttcttca taaagacatc ttctataaat ttggtatgat gagtatcttc
    14581 tctaaactcc tcacttgtta caagtgcctt ttgaaattct acatttgtat taataccttc
    14641 tacaataatt tcgtctagag ctctatatat tctacttata cattcttctc tagtttcagc
    14701 ccaacatatt aatttaccta tcatagaatc atataaaggt ggtatagtat atcctgcata
    14761 tacaaaagta tcaaacctaa caccaaaacc tccaggtaaa tttaatgatt ctatcttacc
    14821 tggtgatgga gcaaaattct ttgatgaatc ttctgcattt attctacatt caatagcatg
    14881 acctctaaaa gtaatatctt cttgtttaaa aggaatttta tccccattag ctatagatat
    14941 ttgaagtttt ataaggtcaa gatttgttac catctctgta actggatgtt caacttgtat
    15001 tcttgtattc atctctataa aataaaagtt tttatctttg tcaactataa actcaatagt
    15061 acctgcaccg atataaccta caccttttac tatatctact gcaattttat gaagtttttg
    15121 tctttcttca tctgaaaggt atggacttaa agattcttct attacttttt gatttcgcct
    15181 ttgcatagaa caatctctat cacctaaatg tattgcattt gaatgtttat ctccaaatac
    15241 ttgtacttct atatgtctag gattttctat aaacttttcc atatataaat cactattaga
    15301 aaatgctgct agagcttctg aacttgccat atcaaaattt gaaaaaagtt cttcttcttt
    15361 tctaacgatt ctcatacctt ttccaccgcc tccacttgag gcttttatca taacaggata
    15421 tccaatttct cttgcaactt ctaaagcttc ttccttagtt ttcaaaacag attctgaacc
    15481 aggaacaaca gggatattca attccatcat agtttctctt gctctagatt tatctcccat
    15541 caaaatcatt tgttctcttg ttggacctat aaaaataata ttgttctctt cacattgctt
    15601 tgcaaattca ggattctctg ataaaaatcc aaagcctgga tgaatagcat cacactttaa
    15661 tttaagagca agatagatta tattttctat attattataa ctcttactta tattatttgg
    15721 tcctatacaa atagattcat cagctatata tctatgaaaa caatctttat ctatttcaga
    15781 atagatagca acagttttta tacctagctc tttacatgtt ctgatgattc taactgctat
    15841 atcgcctcta tttgcaacta gtattttttt tatcaactta aatacctcct actacaatgg
    15901 ttttatctta aataaaggtt gattatattc tactagttct tcattactaa ctaatacttc
    15961 aattatttca cctttaattt cacttgttat ctcattcata agcttcattg cttctaatat
    16021 acataaagtg tcgccttctt caactacatc gccaacttta acaaagctat ctgcatctgg
    16081 acttggagaa tcatagaacg ttcccatcaa aggagctact actactgata gattctcatt
    16141 agatgtgaca ggtgtatcgt ttacaactgg cttttctaaa gaaagatcca ctactgattc
    16201 ttgttgtaca cttaaaacag gtgaacttgt agattgagct tttttagata cttctaatct
    16261 taaatcgcta ctttcaagtt ttacatattc taagttagtt gagtctatgg cctttaataa
    16321 ttccttaatt tcattgatgt tcattttgtt acccccttgt attattacct ggtgctaata
    16381 ttatatcaca aaaaacaaat ttataaaaga aaaagtgctg actagagaat caacacatca
    16441 atatatcttt taattttaaa ttaacaacca gtaacttata tttgttttaa ttttccatta
    16501 ttaatataaa ttataagtat aaaatgaata aaattcaata ttttttgcaa aatacaagag
    16561 aatacaaatt aatacctcgg gaatttttaa gacaaatacc aatattatgc aatttagact
    16621 tcactttctt tcaaattagc atcattttct ttgttcttac ataggataca ctttaattta
    16681 gctattaaaa tatcgctata taggttaaaa gtaattagaa ttgcaggaat tataaaataa
    16741 actctagcac ctataaaacc aaaaactact gtagtaagta ttaatctttt atttaaagta
    16801 aaaaaaggta tattgaaaat acttttgtcg gatgtttctg gagtataaga atatataact
    16861 ccaaaaatca aagtaagcat acaaagtgga agtagtatat aacttgaaat tattcccaga
    16921 aatcctatac tttcaaagta tttgctagca ctatttgtat aaacaaacat gtatccattt
    16981 aagtaggcgc atatacatga tataatgcca agagttcctc ctaaggattg agtgtaccaa
    17041 ttaaaaaatt tagattccat atacctcacc tcctaaaact gtatatataa tgatatgcat
    17101 ttaaatagta aaatatatct ttatatgctt atgaataaaa ttttgttaat ttcattaatt
    17161 tatgtttaag aaatttgaag tatagtgtag tatatattaa tgtaggtaaa cttttatagt
    17221 acttatttta tagtgcttaa tcaagttatc ttattataaa tttgtataga tttaaaagtt
    17281 gttcataggg ttttatattt tgttgagctg aaaaatttat tataaatgaa gggatgtttt
    17341 cgctttggta gatacattag aatttggtct taaaatttta ttttttatat tatcaataat
    17401 ttggatgggt aaaataatga ttttaagaac tgataagcaa atagtaatca atcctttgct
    17461 tattggtata tctgcagtcc ttgtgatgtt gcatacctca cagagtaaca ttgaattttt
    17521 tggattagat gtccagtata taagaatagt tttgtatata atatattcac tgataatttt
    17581 aataggaata tgggctacta acagaagaaa tggaattttt tagataatct atttctataa
    17641 aatttaaagg ttgctagtaa caaagcacta atattagcgt tctgttacta acaaccttta
    17701 ttttgattat acacttaaat tgaaaataga atgattgaat aagttgacat attattttac
    17761 attgacagtg attagaactt cttgagatgt atttaaatca tttttgtcgc ttagtataac
    17821 ttttaccaca gcttgacctg ttttattacc tttaattatc atatcttcac atgaaagtac
    17881 actcttatca cttacagata caataaagtc taaatctttt ggaagattat cagtaacttc
    17941 ccagtattta taataactat caattttctt cataattatt tttctcaaat caatattttc
    18001 accttttgct atatcaacat tatgatattt agagtccagt ttatactgat tatcttttgt
    18061 atcaataaaa tttaaatcgc aatttacatc cacaccatta ttagaaagag aatttactac
    18121 accgtcatca atcaaataat tattggcaag atttattgaa tccatatctt ttagacttaa
    18181 agcttcctta ggaacctctt ttatattatt attccaaaaa ataaaattgt ctacattttc
    18241 aagatatgat atacctttta aatttgaaat gctctcacca gttgcataaa attctccatc
    18301 taaagccttt atattactta tagtcatctc atctgggttt atatatcttt ttacaactgc
    18361 tcttagattt gcatcaggaa aaatatcttt aagttgattt tcgcttaaaa ccttatcatt
    18421 tgaatttatt ggacttaaag ctgctgcaga aataataaat ggtattaaat tagctaaatt
    18481 catagtaccc tcccacataa attttttatt ataataattt aatttctatg agagggttct
    18541 gtttccttgt tatatatacc tactttcgta cactttatta atataaatta aaataaattt
    18601 ctactattaa acttataaac atattactag ttacgttttt tagcttcaca tctaaatatt
    18661 cgcatacctt gtgacataaa tttgtcttca tactctgttg taacattatt ttcatactca
    18721 ctatttccta aatcaagaga aatattcttt aatagccaat tatttgcagc aatttcattt
    18781 aaactaaact cgaataattt ttcattatct gttttaaagt gaatttcgcc atcatcttcc
    18841 aaaaccctat tgtacatttc taaaaatcca gaatgagtga gtcttctttt actccatcta
    18901 tttttaggcc atgggtcaca gaaatttata taaatcctcg ataattcttt agcttcaaag
    18961 taatccaaaa tattgcttac atctccccat aaaaatagta tattattcaa attagaagca
    19021 tctgcttttt caacagcctt taaaagaact tcttccttaa gttccatggc aatataattt
    19081 atgtcagggt tctgcttagc taaagttgtt ataaacttac cttttccagt cccaaattca
    19141 acatgtattg gattatcatt tctaaacttt aaattccact tgccttttaa cttgtctata
    19201 tcgtctctta aaacatattt tgtataactt aaaagctttt catctgcacc tttttttcgt
    19261 cttcttctca caataattct ccttacatat aaaaaattag taaaatacat ttttattata
    19321 cattatctca ccaaaaaata aaaagaactc tcttaaaata gaaatttatt tctataaaga
    19381 gagttctaaa cttactaaaa acttaatctt agtggcaccc tgaacaacca gagcatcctc
    19441 ctccaccttg atccttcata ttttcaggaa taactctaaa tccaaatcct gtatcttcta
    19501 ttattatatc tccatactga ctatattcat cagagctcat tacaaattgt aattctccaa
    19561 cttcataagt taagtcatct tcttttttct catctaaagc taatccaaaa gaaggacctc
    19621 cacaaccaac acctgcaaag tatactctta tattattagg tttatcttga ttgtctttta
    19681 agatactttt taaagtatca atagctgttt ctggtaaagt aattttcatc aaatcattcc
    19741 ctttcaaata ttttattaaa acaatatcta ttatataccc atacaaggta cataatcaat
    19801 tagtttttgc atattttact aaaacttcta cctaatatta cctaaggctt taaaatattt
    19861 tatataatat tttattacct taaataaata ccctatcata aacttagtaa acaaataaaa
    19921 ttattattct aaaaaatata ataattttat ttgttgtttt a
//
LOCUS       DAEPXF010000066        19794 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain HB27
            SAMN13376389-rid12479423.denovo.070, whole genome shotgun sequence.
ACCESSION   DAEPXF010000066 DAEPXF010000000
VERSION     DAEPXF010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13376389
            Sequence Read Archive: SRR10518432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 19794)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19794)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001069624.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001069624.1
            Genome Coverage       :: 315x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/15/2021 01:54:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,165
            CDSs (total)                      :: 4,076
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 89
            rRNAs                             :: 10, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 10 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 19 of 69
            Pseudo Genes (incomplete)         :: 44 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 10 of 69
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19794
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13376389-rid12479423.denovo.070"
                     /strain="HB27"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="China: Hebei"
                     /collection_date="2017"
     gene            51..1118
                     /locus_tag="KRL67_001998"
     CDS             51..1118
                     /locus_tag="KRL67_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HBH1522877.1"
                     /translation="MKNKLIYICIMSLLLFVVGCSNNPNKESTIKKDSKNNQSQVKEE
                     QREPVKKDITISFAGDVTMGNYKGSSYYGSFDHEFKRQGEDYNYFLKNVKPVFEKDDL
                     TVVNLEGPLTTASNAKEKKFAFKGKPGYVNILKSGSVEAVSIANNHSEDYFEEGKKDT
                     QFILDENKINYFGLGKDAVVDVKGIKVGLLGYNGLSTEYNEKNLKQMEDDIKSLKKKS
                     DVVVVYFHWGIELDYYPDKKQKDFAHYAIDKGADLVMGSHPHVIQGIEKYKNKYIAYS
                     LGNFCFGGNKNPSDTDSYIYQQTFTFSDNKLTSIKEPNIIPASITSSSSRNNYQPKVL
                     DGSEKDRVLNKLEKISKDLNK"
     gene            1288..3189
                     /gene="cooS"
                     /locus_tag="KRL67_001999"
     CDS             1288..3189
                     /gene="cooS"
                     /locus_tag="KRL67_001999"
                     /EC_number="1.2.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic carbon-monoxide dehydrogenase
                     catalytic subunit"
                     /protein_id="HBH1522878.1"
                     /translation="MACNTCKMCESADKKLESFVASKDVETAFHRTEDQKVKCGFGLQ
                     GVCCRLCSNGPCRVTPKSPRGICGADADTIVARNFLRAVASGAACYLHVVENTAKNLK
                     NVGITKGVVKGEKTLNQLAEMFGIESDEKYDKCIKIADKVINDLYKSRDDKMELVEKI
                     AYAPRVKKWKELGIMPGGAKSEVFDAIVKSSTNLNSDPVDMLVNCLNLGISTGLYGLT
                     LTNLLNDVMLGEPVIRMAPVGFNVIDPDYINIMITGHQHSTFANFQDRLKDEDVIKLA
                     QSVGAKGFKLVGCTCVGQDLQLRGEHYQEVFAGHAGNNFTSEAVLSTGAIDIVLSEFN
                     CTIPGLEPIADKYKVKMVCLDDVAKKANAEYIGLDRSKLDELSNTLIEKALESYKERR
                     GSIEIDIPKDHGFEQSLTGVSEKNLKAFLGDSWKPLINLIAEGKIKGVAAVVGCSNMT
                     AGGHDVNTVELTKELIKKDIIVLSAGCSTGGLENVGLMSPGAEELAGENLKEVCKTLG
                     IPPVLNFGPCLAIGRLEIVATELAAELGIDLPQLPLVLSAPQWLEEQALADGAFGLAL
                     GLPLHLALPPFITGGKLVTEVLTEKLKDLTGGHVIVNPDPKSSANQLEEIIIDRRKNL
                     GLKDVECNA"
     gene            3182..3655
                     /locus_tag="KRL67_002000"
     CDS             3182..3655
                     /locus_tag="KRL67_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HBH1522879.1"
                     /translation="MHRIFINKDLCTGCKSCVLACMLKHNKDYDMYTLDLENIDNDSR
                     GHIELDSKHNNPVPILCRHCDEPECVLACMSGAMHKDSESGIVSYDEEKCGSCYMCVM
                     SCPYGLLKPDDRSKQNILKCDLCKDEEYPRCVANCPSGAIELQKEENDELCSVRG"
     gene            3630..4856
                     /locus_tag="KRL67_002001"
     CDS             3630..4856
                     /locus_tag="KRL67_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HBH1522880.1"
                     /translation="MNYVVLGASAAGINAVKTLRELDKDSNIVVISKDENIYSRCMLH
                     HVISEHRTLKQINFVDEDFMEQNNVKWIAGKTVKGIDINKKVVQTEDITVNYDKLLIA
                     TGASSAIPPIKNLRDGNFVYSVRNIDDIYKIKEKAQNSKNVVIIGAGLVGIDALVGLF
                     KYEQLNISVAFMEKYILDRQLDEYTASVYENKFKEKGVKFYPSASIQEIVLDNSKNVK
                     GVAFSNGEILDADMVIVATGVKPNADFLDETGIEYDRGIIIDDMCQTTQKDIYAAGDV
                     VGKNAIWPLAVKQGIVAAYNMVGKDKKIEDEFAFKNSMNFMDIPTISIGMNTPVDDSY
                     KVLTRHGINDYKKFIFKDNVIYGAVIQGDISYVGVLTYLIKNKVEIYDLENRIFDVCY
                     ADFFNIKENGEFCYSV"
     gene            complement(4995..6182)
                     /locus_tag="KRL67_002002"
     CDS             complement(4995..6182)
                     /locus_tag="KRL67_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HBH1522881.1"
                     /translation="MNITKNFLKDYLERFGETPFLVRLKDEEEFLIGEGSPQFKVIVN
                     NDISKSELLKSTSLALGEAYINRDIEVEGDLFLTLNLLLKQIDKFNIDNSAVKHLLHS
                     SNSMRHQKKEIHAHYDIGNDFYSLWLDKTLSYSCAYFRNDNDTLYDAQVNKVHHILTK
                     LNLEEGMSLLDIGCGWGFLLIEAAKKYKIHGVGITLSEEQFKEFNARIKEENLQDYLE
                     VRLMDYRELKKSDLMFDRVVSVGMLEHVGRENYELFIKNVNAVLKDGGLFLLHYISGL
                     QESYGDPWIKKYIFPGGVIPSLREMIHICSDYKYHTIDVESLRLHYVKTLLYWQKNFS
                     KNLPKIKEMFDDKFIRMWDLYLCSCAAAFNTGIIDIHQILFTKGIKNDLPLTREYMYN
                     CSK"
     gene            6455..7279
                     /locus_tag="KRL67_002003"
     CDS             6455..7279
                     /locus_tag="KRL67_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBH1522882.1"
                     /translation="MEVYALVGSSGTGKSFKALEFAYENDIEYIIDDGILIYKNKVLA
                     GISAKQANTTIEAVKRAIFYNLEHRQEVRETIKKENIRKILIIGTSKKMVNQIVERLS
                     IGRVCNFINIQDISTKYEIEIAKQARKEGNHVIPVPAVEIKSMASGLSINSLKRLFRK
                     GNNRNMVVLEKTIIRPTFSYIGKFYISADVIKQIIEYEVYNFGSIDRINKINIENCNN
                     FMNIFVSININDLETIKKIEDIQQTIKKSIEKMTLVNVQKIDINIHKIKKINFLQC"
     gene            7408..8673
                     /gene="gluD"
                     /locus_tag="KRL67_002004"
     CDS             7408..8673
                     /gene="gluD"
                     /locus_tag="KRL67_002004"
                     /EC_number="1.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-specific glutamate dehydrogenase"
                     /protein_id="HBH1522883.1"
                     /translation="MSGKDVNVFEMAQSQVKNACDKLGMEPAVYELLKEPMRVIEVSI
                     PVKMDDGSIKTFKGFRSQHNDAVGPTKGGIRFHQNVSRDEVKALSIWMTFKCSVTGIP
                     YGGGKGGIIVDPSTLSQGELERLSRGYIDGIYKLIGEKVDVPAPDVNTNGQIMSWMVD
                     EYNKLTGQSSIGVITGKPVEFGGSLGRTAATGFGVAVTAREAAAKLGIDMKKAKIAVQ
                     GIGNVGSYTVLNCEKLGGTVVAMAEWCKSEGSYAIYNENGLDGQAMLDYMKEHGNLLN
                     FPGAKRISLEEFWASDVDIVIPAALENSITKEVAESIKAKLVCEAANGPTTPEADEVF
                     AERGIVLTPDILTNAGGVTVSYFEWVQNLYGYYWSEEEVEQKEEIAMVKAFESIWKIK
                     EEYNVTMREAAYMHSIKKVAEAMKLRGWY"
     gene            8866..9567
                     /locus_tag="KRL67_002005"
     CDS             8866..9567
                     /locus_tag="KRL67_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M56 family metallopeptidase"
                     /protein_id="HBH1522884.1"
                     /translation="MDFFPPHDYKLEELDWIFRHELMHFKSKDLHLKYLVLFLKIVYW
                     FNPFIYIMEKYMDLDCELYCDERVLKNCTVQQKQEYALTIVNSMKRGINFSNKFVAGL
                     NKQSDIRKRVINMFSEKHKNGILMALIISLFSSITFFKFDSTSIIEENDFLERSRYTF
                     EGRQEDMFVTIDFTYADAPIKLRKEHEENCRANEIVPKDSGSIEIIPRFYKDFMDLKN
                     KLDLILMLILLIFYI"
     gene            9852..10985
                     /locus_tag="KRL67_002006"
     CDS             9852..10985
                     /locus_tag="KRL67_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M56"
                     /protein_id="HBH1522885.1"
                     /translation="MSNFILNGIFRTVIVSSLSILLILIFKKNVFKRFSKKFNYYIWM
                     IVVIKLFLPFTYYTFTINILRSKKHININNINLEGFNNVSTIGNSILLYTWIITVVVY
                     LIYTIFKYIKLKNLINDLSYDVDDEEIVNLYKNILEEFNITKDIKLKYSYEVETPAFF
                     NSCVLLPPHEYKLKELYWIFRHELMHFKSRDLYLKYVILFLKTVYWFNPFIYIMDKHI
                     DLDCELYCDERVLKNRNSEEKKDYALTILNAMRKGSNTSNKFIAGLHKQSDIKKRVMN
                     MFNEKYKNGILMALTLCLLSSITFLKVNSTSIVNLNILPKGMEEKDVTNSAIVTMIEF
                     TYAEAPKSMREEHKINCEFLGLIPKDSDKIGVGVEYYDSLLND"
     gene            11902..12900
                     /locus_tag="KRL67_002007"
     CDS             11902..12900
                     /locus_tag="KRL67_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HBH1522886.1"
                     /translation="MKKKILIPVFASVMALSVSSIVNADEVNDSSQNKDDKTNTELNL
                     GEYKEVKYKVAKIKDGVAIKIREEGQVQNIAYSGDEFTVLGTQGEWVKVKVEDGEGWL
                     ATRYVDISEGVGYTNADKVNLRKDKSESSEVIEELEKGSSLLVLEDNGDWLKVKDGET
                     EGYVKSSYISDKAPVIEEPQVNPSINQNVDSTNTNQQNNNSNVPTANSNAVQAVLNLA
                     YSKQGCPYVWGAEGPNTFDCSGFTQYVYRNAVGKNIPRTSKAQSKYGQTVSKANLQPG
                     DLVFFTTNGSGSVSHVGIYVGGGNMIHSPSTGKTVSVTSINSSYYTARFVTAKRIL"
     gene            13197..14117
                     /gene="pyrB"
                     /locus_tag="KRL67_002008"
     CDS             13197..14117
                     /gene="pyrB"
                     /locus_tag="KRL67_002008"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase"
                     /protein_id="HBH1522887.1"
                     /translation="MLKSRNLIQPEDFSIEEIDEILELAQKIIDNPSKYSRICEGKLL
                     ATLFYEPSTRTRLSFESAMNRLGGRVVGFSEPNSSSASKGETLGDTMRIVSGYVDIIA
                     MRHPQSGAASEAARYTEVPFINAGDGKNQHPTQTLTDLLTIKSLKGTLESHTIGLCGD
                     LKYGRTVHSLVKAMARYKNTKFVFIAPEELKMPDYIKEAIKGHAYYETNNLDDVIGSL
                     DVLYMTRVQQERFEDKSEYERLKNYYILNKAKLEKASKNMLVMHPLPRVNEIDIDVDS
                     DDRAVYFKQAKYGMYVRMALIIKLLGISED"
     gene            14156..14848
                     /locus_tag="KRL67_002009"
     CDS             14156..14848
                     /locus_tag="KRL67_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HBH1522888.1"
                     /translation="MYKILENIYIGEDMYRMKVKGNFEGKMGQFYMLRAWDTYPVLSR
                     PISIHDIDEEGITFLYKVVGEGTQILSNLKVNDTIKLEGPYGNGYAKVDGKVALVGGG
                     IGVAPLYLVAKNIKNCDAYLGFREDVILEDEYKQVCNKVYTTVGNTFVTDIIDVEKYD
                     YILTCGPTLMMEKLVKMVEGTKTRIMVSLENHMACGVGACLVCTCKTNEGNKKTCKDG
                     PVFWGEDVIFNG"
     gene            14841..15743
                     /locus_tag="KRL67_002010"
     CDS             14841..15743
                     /locus_tag="KRL67_002010"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HBH1522889.1"
                     /translation="MGNLSVKFGSVEFKNPVIMASGTFGFGKEYNEIYDIQKLGGISS
                     KGLTLNKKPGNNGMRVHETPSGMMNSVGLENPGVQGFIDNELPFFSKLDLVRIANVGG
                     GTLEDYLLGVQMLNDKPIDIIELNISCPNVKAGGMAFGIKNEVAREVVREVRNITKLP
                     LVIKLSPNAEDIVGMAKVCEEEGADGVSLVNTFKAMAIDIKNRRPVFENVYAGLSGPA
                     IKPIALRMVHEVCKNVNIPVMGMGGITKATDAIEFIMAGATCIQVGTANFMNPRIGIE
                     IIDGINEFMDREGIKSLDEIIGII"
     gene            15828..16412
                     /locus_tag="KRL67_002011"
     CDS             15828..16412
                     /locus_tag="KRL67_002011"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HBH1522890.1"
                     /translation="MSKVDVVDILKKSDALLEGHFLLSSGKHSNRYVQCAKVLRFPEY
                     AAEVLSTVVEQIKDLDIDLVVGPAMGGVIVSYELGRQLGKEAVFTERKDNTMELRRGF
                     EVKKGAKIIIAEDVVTTGKSTMETKRVLEALGGEVVGVACIADRTNHDIGMPIYSAIK
                     LDIQVYESDECPLCKEGKLPVVKPGSREFKELGM"
     gene            16628..17554
                     /locus_tag="KRL67_002012"
     CDS             16628..17554
                     /locus_tag="KRL67_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketopantoate reductase family protein"
                     /protein_id="HBH1522891.1"
                     /translation="MKNQLNENHKKVVFFGVGAVGATFAEQFFNSKYDFKILCDNERK
                     KRYLEEGFIINGKRYDFDYVTKDEYKQEADFIIIGLKYNNLKENIKELDGLVGKNTVI
                     MSLLNGVDSEEIIGERFGIEKMVYSYVTNIDAKKINNNIIHTTNGVIVFGNKDNSEDR
                     KTNIITEIFDDVNIEYILSKDIQRDMWWKYMVNIGVNQTSAILGAPYGVFQSSEHLRE
                     LAKSAMREVVAIAQAKDISLTEDDVEHSLHRILEHSKEGRTSMLQDVEAHRPTEVDMF
                     SKNICKLGKKYNIPTPINQTFFYMIKVIESRF"
     gene            complement(17606..18496)
                     /locus_tag="KRL67_002013"
     CDS             complement(17606..18496)
                     /locus_tag="KRL67_002013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBH1522892.1"
                     /translation="MFEELKTFVAVVEYKNFTKAGEYLNLSQPSVSKHIKNLENYFKV
                     VLINRSIKQKTIFITESGQILYKRAKEILNLLNITYHDVSQVSDAITGHLKIGASLTI
                     GEYILPNFLALFSKKYPDIDVEVFIKNTSIVSSHVKDYILDIGLIEGTCSSPSFIQEY
                     FFEDKMVLALPYNSHLLKDFSFDKLQNQKWIVREDGSGTRDYLDMFLSVKEIIPKSMM
                     VFGSNYAVKESVRNNLGITIVSNLVTSLPVLNNELSVIELGSSYNRHFSYIFPKDITL
                     SKAATIFIEELKIFSNLNSI"
     gene            18684..19715
                     /locus_tag="KRL67_002014"
     CDS             18684..19715
                     /locus_tag="KRL67_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative sulfate exporter family transporter"
                     /protein_id="HBH1522893.1"
                     /translation="MLKKKSSLTLKTIREILPGLFVSVLVGYISIFISILIPKVGAAS
                     ISIFLGMFVGNLFLNQKVFQKGYKFSETDLLSYSIVLLGATLSVSTLIDLKVSGILFI
                     ILQMTITIIAALYIGKKLGFEENFRFLMASGNAVCGSSAIGATVPVVNATDKEKGIAV
                     TIVNVTGIFLMFLLPIISQFLYSHELVKTSAMIGGTLQSIGQVVASGAIVGENVKDLA
                     TIFKIVRVIFLVVVVLVFGHMKNQSNREILEEEKDEIKKKKVTIPWYVIGFFITCALF
                     SMNIIPKEVSVLCKEISNKLEIIALAAIGLKVNVKDLVKQGKEVSLYGLFVGTVQVVS
                     AVVLIKIFI"
BASE COUNT         7780 a         2087 c         3506 g         6421 t
ORIGIN      
        1 taaaaaagta tattttattt aaaaatttat taatacaaag gagatttact atgaaaaata
       61 aattaattta catatgtatt atgagtctat tgctatttgt agtaggatgt tctaataatc
      121 caaataagga aagtaccatt aaaaaagact ctaagaataa ccaaagccaa gttaaagaag
      181 aacaaagaga acctgtgaaa aaagatataa ctataagttt cgctggtgat gtaactatgg
      241 ggaactataa aggttcatca tattatggaa gttttgacca tgaatttaaa agacaaggag
      301 aagattacaa ttattttctt aaaaatgtaa aacctgtatt tgaaaaagat gacttaacag
      361 tggttaattt agaaggtcca cttactacag cgtcaaatgc aaaagaaaaa aaatttgcat
      421 ttaaaggaaa gcctggatat gtaaatattt taaaatctgg aagtgtagag gcagtaagta
      481 ttgcaaataa tcattcagaa gattattttg aagaaggaaa gaaagataca cagtttatat
      541 tggacgagaa taaaataaat tattttggat tagggaaaga tgctgttgta gatgtcaaag
      601 gaattaaagt gggtctatta gggtataatg gattatcaac tgaatataat gaaaaaaact
      661 taaaacaaat ggaagatgat attaagtcct taaaaaagaa gtctgatgtt gttgtagtgt
      721 actttcattg gggaatagaa ttagattatt atccagataa aaaacaaaaa gattttgctc
      781 attatgctat agacaaggga gcagatttag ttatggggtc tcacccacat gtgattcaag
      841 gaatagaaaa atataaaaat aagtatatag cttatagtct agggaatttt tgctttggag
      901 gaaataagaa tccttctgat acagatagtt atatatatca acaaaccttt actttttcag
      961 ataataaatt aacttcaatt aaagagccaa atataatacc agcttcaata acatctagta
     1021 gctccagaaa taattatcaa ccaaaagtat tggatggaag cgagaaggat agagttctaa
     1081 ataaacttga aaaaataagc aaagatttaa ataaataaaa tgtaaaaaaa aataaatata
     1141 tatatttatt ttaataattt atattttgtt tagggtatgg gaaatcgtgg tattaaataa
     1201 gtagatttta atagttaaat tatagtcaat tataataaag ctatttaatc tactttttta
     1261 atataaaaat ataaggggga atttgtaatg gcttgtaaca cttgtaaaat gtgcgaaagt
     1321 gcagacaaga aattagaaag ttttgtagct tcaaaggatg ttgaaacagc atttcataga
     1381 acagaagatc agaaagttaa gtgtggtttt ggacttcagg gggtttgttg tagactctgt
     1441 tcaaatggac cgtgtagagt aactcctaaa tcaccaagag gtatatgtgg agcagatgca
     1501 gacactatag tagcaagaaa ctttttaaga gcagtagcat caggagcagc ttgttattta
     1561 catgtggtag aaaatacagc taagaattta aaaaatgttg gaataactaa aggtgttgtt
     1621 aaaggagaaa aaacattaaa tcaattagca gagatgtttg gaatagaaag tgatgaaaag
     1681 tatgataaat gtataaagat tgcagacaaa gttatcaacg atttgtataa atcaagagat
     1741 gataaaatgg aattagtaga aaaaatagct tatgcaccta gagtgaaaaa gtggaaagag
     1801 ttaggtataa tgcctggtgg tgcaaagtca gaagtttttg acgcaatagt aaaatcatca
     1861 acaaatttaa acagtgaccc agtagacatg ctagttaatt gtttgaactt aggtatatca
     1921 acaggattat atggtcttac actaactaat cttttaaatg atgttatgtt gggagaacca
     1981 gttataagaa tggctcctgt aggatttaat gtaatagacc cagactacat aaatataatg
     2041 ataacaggtc atcaacattc aacatttgca aatttccaag atagattaaa agatgaagat
     2101 gttataaaat tagctcaatc tgttggtgca aaaggattta agctagtagg atgtacatgt
     2161 gttggtcaag atttacaatt aagaggagaa cattatcaag aagtatttgc tggacatgca
     2221 ggtaacaatt ttacaagtga ggctgtttta tcaactggag caatagatat agttctttca
     2281 gaatttaact gtactatacc agggcttgaa ccaatagcag ataaatataa agtaaaaatg
     2341 gtttgtttag atgatgttgc taaaaaagcg aatgcagaat acataggttt agacagaagt
     2401 aaattagatg aacttagtaa cactttaata gaaaaagcat tggagtctta taaggagaga
     2461 agaggaagta ttgaaataga tataccaaaa gaccatggat ttgaacaatc tttaactggt
     2521 gtaagtgaaa agaacctgaa agcgttttta ggagattcat ggaaacctct tattaattta
     2581 attgcagaag gaaaaattaa aggtgttgca gctgttgtag gatgttcaaa tatgacagca
     2641 ggaggacatg atgtaaatac tgttgaactt actaaagaat tgattaaaaa agatataata
     2701 gtactatctg caggatgttc tactggagga ttagaaaatg ttggtcttat gtcacctgga
     2761 gcagaagaat tggcaggaga aaacttaaaa gaagtatgta agactttagg aataccacca
     2821 gtattaaact ttggaccatg tctagctata ggaagactag aaatagtagc aacagaatta
     2881 gcagctgaac ttggaattga cctaccacaa ttaccattag tgctttcagc tccacaatgg
     2941 ttggaagaac aagcattagc tgatggagct tttggtcttg cccttggctt acctcttcat
     3001 ttagctttac ctccatttat aacaggagga aaattggtaa cagaagtatt aacagaaaaa
     3061 ttaaaagact taacaggagg acatgttata gtgaatccag accctaaatc ttcagctaat
     3121 caattagaag aaataataat agatagaaga aaaaatttag ggttaaagga tgtggaatgt
     3181 aatgcataga atttttataa ataaagattt atgtacagga tgtaagagct gtgtactagc
     3241 atgtatgcta aaacacaata aagattatga catgtacact cttgatttag aaaatataga
     3301 taatgatagt agagggcata tagaattaga tagtaaacat aataatccag ttccaattct
     3361 ttgtagacac tgtgatgaac ctgaatgtgt attagcatgt atgagtggtg caatgcataa
     3421 agatagtgaa agtggaatcg tttcatatga tgaagagaaa tgtggttcat gttatatgtg
     3481 tgtaatgtct tgtccatatg gattattaaa gccagatgat agaagcaagc aaaacatact
     3541 taaatgtgat ttatgtaaag atgaggagta tcctagatgt gtagctaatt gtccaagtgg
     3601 agcaattgaa ttacagaagg aggaaaatga tgaattatgt agtgttaggg gctagtgcag
     3661 ctggtataaa tgcagtaaaa acattaagag aattagataa agattcaaat atagtagtaa
     3721 tttctaaaga tgaaaacata tactcaagat gtatgctgca tcatgttata tctgaacaca
     3781 gaactttaaa acaaataaat tttgttgatg aagattttat ggagcaaaat aatgtaaaat
     3841 ggattgcagg taagactgtt aaaggtatag acataaataa aaaagtagtt cagacagaag
     3901 acattactgt aaattatgat aaacttttaa tagctaccgg tgcatcttct gctattcctc
     3961 ctataaaaaa tttaagagat ggaaactttg tatattctgt gagaaacata gatgatattt
     4021 ataaaataaa agaaaaagca caaaattcta aaaatgtagt tataataggt gctggtttag
     4081 ttggtataga tgcactagtg ggattattta aatatgagca gttaaatatt tctgtagctt
     4141 ttatggaaaa gtacatttta gatagacaat tggatgaata tacagcatca gtgtatgaaa
     4201 ataagttcaa agaaaaaggt gtaaaatttt acccaagtgc atcaatacaa gaaattgtat
     4261 tggataacag taaaaatgtt aaaggtgttg cattttcaaa tggtgaaatt ttagatgcag
     4321 acatggtaat tgtagctaca ggggttaaac caaatgctga ctttttagat gaaacaggaa
     4381 tagaatatga tagaggcata ataatagatg atatgtgtca aacaacacaa aaagacatat
     4441 atgcagcagg agatgttgta ggtaaaaatg ctatttggcc tctggcagta aagcaaggaa
     4501 ttgtagctgc atataatatg gttggaaaag ataagaagat agaagatgaa tttgctttta
     4561 aaaatagtat gaatttcatg gatataccaa ctatttctat aggaatgaat acacctgtag
     4621 atgatagcta taaagtatta accagacatg gaattaatga ttataaaaag tttattttta
     4681 aagataatgt aatctatgga gcagttatac aaggagatat ttcatatgta ggtgtattaa
     4741 cttatttaat aaaaaataaa gttgaaatat atgatttaga aaataggata tttgatgtat
     4801 gttatgcaga tttttttaat ataaaagaaa atggagaatt ttgttatagc gtatagtata
     4861 tacatggagt aaggtttata aatacagtaa atcaataaat agagttattc taaaataaga
     4921 aaaatgacaa atcttattta gaaatagctc tattttttgt aattaagttt aaattatatt
     4981 caatttgtaa aagattattt tgaacaatta tacatatact ctctagttag aggtaaatca
     5041 ttttttatgc ctttagtgaa aagtatttga tgtatatcta taataccagt gttgaaagca
     5101 gcagcacaag aacaaaggta taagtcccac attcttataa acttatcatc aaacatttct
     5161 ttaatctttg gaagattctt agagaagttt ttttgccagt atagtaaggt ttttacataa
     5221 tgtaatctta gactttctac gtctatagta tgatacttgt aatcactaca aatatgtatc
     5281 atttctctta ggctaggaat aactcctcct gggaaaatat atttttttat ccaagggtct
     5341 ccataagatt cctgcaatcc acttatatag tgaagtaaaa ataatccacc atcttttaaa
     5401 acagcattta cgtttttaat gaaaagttca tagttttctc taccaacatg ttcaagcatc
     5461 ccaacgctta caactctatc aaacataagg tctgattttt ttaattctct atagtccata
     5521 agtcttacct caaggtagtc ttgaaggttc tcttctttaa ttcttgcgtt aaattcctta
     5581 aattgctctt cgctaagcgt aattccaacg ccgtgaattt tatatttttt tgcagcttct
     5641 attagtaaaa atccccaacc acaaccaata tcgagaaggc tcataccttc ctctaaattt
     5701 agtttagtta atatgtggtg aactttattg acttgagcat cataaagagt atcattatca
     5761 tttctaaagt atgcacaaga ataactaagt gtcttatcta accataagct ataaaaatcg
     5821 ttaccaatgt catagtgggc atgtatttct ttcttttgat gtctcattga atttgatgag
     5881 tgcaatagat gttttacagc tgaattatca atattgaatt tgtctatttg ttttaaaagt
     5941 agatttaaag ttaagaataa atctccctca acttcaatgt ctctatttat gtaggcttct
     6001 ccaagtgcta aagatgtact ttttaatagt tcagacttgc taatatcatt atttactata
     6061 actttgaact gaggggagcc ctctccaatt agaaattctt cctcatcttt aagcctaact
     6121 agaaatggtg tttcaccaaa tctctctaaa tagtctttta aaaaattttt tgtaatattc
     6181 atattaccag ctccttctaa tgtaaatcca gtaagtttcc ctgcttaaaa tatattatag
     6241 tccttttttt aaaaaagtaa aattttttca ataaattttt tatttagagg aaatttattt
     6301 tttataaaga aatttcatgt tgtagtatat tttttgccta taaattttgg tactggatat
     6361 aatattaaac tgttaatgat ataataaatt taatctataa agtaaataaa attttgtaat
     6421 aaatagttaa agctatttta taaggagata taggatggaa gtatatgctt tggttgggtc
     6481 aagtggcaca gggaagagct ttaaggcttt agagttcgca tatgaaaatg atatagaata
     6541 tataatcgat gatgggattt taatatacaa aaacaaggtt ttagctggga tatcggcaaa
     6601 acaagcaaat actactatag aagcagttaa aagggctatt ttttataatc tggagcatag
     6661 acaagaagtt agagaaacaa ttaaaaaaga aaatataaga aaaattctta ttattgggac
     6721 atcaaaaaaa atggttaatc aaattgtaga gagattgtca attggaaggg tctgtaactt
     6781 tataaatata caagatataa gtactaaata tgaaatagaa atagcaaaac aagctagaaa
     6841 agaaggaaat catgtaattc ctgttccagc agttgagata aaaagtatgg caagtggtct
     6901 tagtataaat tctttgaaga gactttttag aaaaggaaat aataggaaca tggtagtttt
     6961 agaaaaaaca attataagac caacttttag ttatatagga aagttttata ttagtgctga
     7021 tgtaattaaa cagattattg aatatgaagt atacaacttt ggaagcattg atagaataaa
     7081 taaaatcaat atagagaatt gcaataattt tatgaatatt tttgtaagta taaatatcaa
     7141 tgatttagaa actataaaaa aaattgaaga tatacagcag acaataaaaa agtctattga
     7201 gaaaatgact ttagttaatg tacaaaaaat agatattaac attcataaaa ttaaaaaaat
     7261 aaacttcctc caatgttaaa aaataaactg agaaaatgat atactaattt gcaataaagt
     7321 gtgctataat taaactgtaa atttaggaaa tggttttttc taaatggagt atacataatt
     7381 ataacgtatt tataaggggg ctttcaaatg tcaggaaaag atgtaaatgt cttcgagatg
     7441 gcgcaatctc aagtaaaaaa tgcatgtgat aaattaggta tggaaccagc agtttatgaa
     7501 ttattaaaag aacctatgag agttatagag gtttcaattc cagtaaaaat ggatgatggt
     7561 tctataaaaa cttttaaagg atttagatca caacataatg atgcagtagg gccaacaaaa
     7621 ggtggaataa gattccatca aaatgtttca agagacgaag taaaagcttt atctatatgg
     7681 atgactttca aatgttctgt aacaggtata ccatatggtg gaggtaaagg tggaataata
     7741 gtagatccat caactttatc tcaaggtgaa ttagaaagat taagtagagg atacatagat
     7801 ggaatatata aattaatagg tgagaaagtt gacgttcctg caccagacgt aaatactaat
     7861 ggtcaaataa tgtcttggat ggttgatgag tacaataaat taactggaca aagttctata
     7921 ggtgttataa ctggtaagcc agttgaattt ggtggttctt taggaagaac agctgctact
     7981 ggatttggtg ttgctgttac tgcaagagaa gctgctgcta aattaggaat agacatgaaa
     8041 aaagctaaaa tagctgttca aggtatagga aacgttggtt cttatacagt tcttaactgt
     8101 gaaaaacttg gtggtactgt tgtagctatg gctgaatggt gtaaatcaga aggttcttat
     8161 gctatataca atgaaaatgg tttagatggt caagctatgt tagattatat gaaagaacat
     8221 ggtaacttat taaacttccc aggagctaag agaatatctt tagaagagtt ctgggcttca
     8281 gatgttgata tagtaatacc agctgcatta gaaaactcta taactaaaga agttgctgaa
     8341 tctataaaag ctaaattagt ttgtgaggct gctaatggac caactactcc agaggctgat
     8401 gaagtatttg ctgaaagagg aatagttctt actccagata tattaactaa tgctggtgga
     8461 gttacagttt cttacttcga gtgggtacaa aacttatatg gatactactg gtcagaagaa
     8521 gaagtagagc aaaaagaaga aatagctatg gttaaagcat ttgaatctat ttggaaaatc
     8581 aaagaagagt acaatgttac aatgagagaa gctgcttaca tgcattcaat taagaaagtt
     8641 gctgaagcta tgaaattaag aggatggtac taggatttac tcttaataaa taataggata
     8701 taattattta agatataaag gggctgtaca tagtgcagcc cttaaattat gtagcttaca
     8761 atgaagtgag ctatttttat tttaacttat aaataacttg ataaattatg taatttagaa
     8821 tgcataaata agatgggtga aaattaatgg ttgatttaat tttgaatgga ttttttccca
     8881 ccacatgatt acaaattaga agagttagat tggattttta gacatgaact tatgcatttt
     8941 aaaagcaaag accttcattt aaagtatcta gtattatttt taaaaatagt atattggttt
     9001 aatccattta tatatataat ggaaaaatat atggatttgg attgtgaact ttattgtgat
     9061 gagagagttt taaaaaattg tactgtacaa caaaagcaag aatatgctct aactattgta
     9121 aattcaatga aaagaggtat aaatttttca aataaatttg tagctggatt aaataaacaa
     9181 tctgatatta gaaaaagagt aataaacatg ttcagtgaaa aacataaaaa tggtatctta
     9241 atggctttga ttataagctt gttttcttct attacatttt ttaaatttga ttcaactagt
     9301 attattgaag agaatgattt cttagagagg tcaagatata cgtttgaagg aaggcaagaa
     9361 gatatgtttg taacaataga ttttacatat gcagatgcac ctataaaact tagaaaagag
     9421 catgaagaaa actgtagagc caatgaaata gtaccaaaag attcaggttc aatagaaatt
     9481 attcctagat tttataaaga ttttatggat ttaaaaaata agttagactt aatattgatg
     9541 ttgatattat taatttttta tatttaagta attgaaaatt ttattatgga ttcaatttta
     9601 tatgtgttta agtatgaaat ttatagatag ttgaaataat taaatatgat tgatgcatag
     9661 gaatcattac taagctataa ttaattataa gggagtttaa ataaattatg agttacacaa
     9721 ttataaaatg ataaaaaata gttatctgtt acaaaaaatg tatcttttta attaataaag
     9781 ataatatgaa aacttaaaag gtaaataaac ttaaatataa aatactctta aataagatag
     9841 gagaaaaatg tatgtctaac tttattctaa atgggatttt tagaacagtt atagtaagta
     9901 gtctaagcat tcttctgata ttgattttca agaaaaatgt atttaaaaga tttagcaaaa
     9961 aatttaatta ttatatatgg atgatagttg ttattaaatt atttttacca tttacatatt
    10021 atactttcac aataaatatc ttgagaagta aaaaacacat taatattaat aacataaatt
    10081 tagagggatt taataatgtt tcaactatag gtaatagtat tctattgtac acttggatta
    10141 taactgtagt agtgtattta atttacacta tatttaaata tataaaactt aaaaatttaa
    10201 taaatgactt atcttatgat gttgatgatg aagagattgt aaatctttat aaaaatatat
    10261 tagaagaatt taatataaca aaagatataa aactcaaata ctcttatgaa gttgaaacac
    10321 cagcattttt taattcatgt gtgcttttgc caccacatga gtataaattg aaagagttat
    10381 attggatttt tagacatgag cttatgcatt ttaagagtag ggacctttat ttaaaatatg
    10441 taatcctgtt tttaaaaaca gtatattggt ttaatccatt tatatatata atggataaac
    10501 atatagattt agattgtgaa ctttattgtg atgaaagagt tttaaaaaat cgaaattcag
    10561 aagaaaaaaa ggattatgct ttaacaattc taaatgcaat gagaaaaggt tcaaatactt
    10621 caaataaatt tatagctggc cttcataagc aatctgatat taaaaagaga gttatgaata
    10681 tgtttaatga aaagtataag aatggtatct taatggcatt aactttatgt ttattatctt
    10741 ctattacttt tttgaaggtt aattcaacta gtattgttaa tcttaatata ttgccaaaag
    10801 gaatggaaga aaaagatgtt actaatagtg caattgttac aatgatagaa tttacatatg
    10861 cagaagctcc taaaagtatg agagaagaac ataaaataaa ttgtgaattc ttaggtttaa
    10921 tacctaaaga ctctgataaa ataggtgttg gtgtagaata ttatgacagt ctattgaatg
    10981 attaaataaa tattatttta acagatatta attataaagt tttgaatagt aatttgttga
    11041 gaaatagata tatccttata agaatagata cttatatacc aagaataaaa aatagtcaag
    11101 atagtacgca aggatatact tataaagaac ttaagtaagt gaaataaatt attaattgta
    11161 aggtttataa tttaaaaaaa atagataaat tcaggtgtaa aatacaattg ggaaaagtgt
    11221 atgtcgaact tcatttaaaa tggaattttc aaagttgtta tactaagtag tttaagcatt
    11281 cttcttatat taatctttga taataatgta tttaaaaaag ttagtaaaaa atttaactat
    11341 tatatcttaa tgatagttac tattgatata ttatctaaaa aaattaagta tgaaaagatt
    11401 actgctagga caatcttttc atacttaatt tttgaatatt tatattctcc aaaattttca
    11461 tctttttaac ttagaattat tagaatttga gaatcactat taggaaatag gctttgattt
    11521 atgtagggtt aaaaatttca cagaatatct aactttatac taaatccatc aaaaaatata
    11581 cgacatataa aagtgcataa aaaaacacat aaaacaatat ttatgcgcac tttaaccttg
    11641 aatggtacaa aattctttac ttttatagac ccatattata taattaagta aataatgtat
    11701 atttaggtga catattggat aagctaattt atttatttta ttaatagaat gatactataa
    11761 aagttatata aagtggttta caaaaaagtt acagatagag cttttattaa tgtattgtgt
    11821 attagtattt tatccttagg tacacttcac ctacaaaata gaatcataag ataattatgg
    11881 gaaaatttta ggaggtttat cgtgaagaaa aagatattaa taccagtatt tgcatcagtt
    11941 atggcattat cagtaagttc aattgtaaat gcagatgaag taaatgattc atctcaaaat
    12001 aaggatgata aaacaaatac tgaattaaat ttaggtgaat ataaagaagt aaaatataaa
    12061 gttgcaaaaa taaaagatgg agtagctata aagataagag aagaaggtca agttcaaaat
    12121 atagcttatt ctggagatga atttactgtt ttaggaactc aaggagaatg ggttaaggtt
    12181 aaagttgaag atggtgaagg ttggttagct actagatatg tagatatatc tgaaggtgta
    12241 ggatatacta acgctgataa agtaaactta agaaaagata agagtgaaag ttcagaagta
    12301 attgaagagt tggaaaaagg aagttcttta ttggttttgg aagataatgg agattggtta
    12361 aaagttaaag atggggaaac agaaggatat gtaaaatcat cttatatatc tgataaagct
    12421 cctgtaatag aagagccaca agtgaaccca agtattaacc aaaatgtaga ttctacaaat
    12481 actaatcaac aaaataataa ttcaaatgtg ccaacagcta actctaatgc agttcaagct
    12541 gtacttaact tggcatattc taagcaaggt tgcccatatg tttggggagc agaaggacca
    12601 aacacttttg actgttctgg atttacacaa tatgtgtata gaaatgcagt aggaaagaat
    12661 ataccaagaa catctaaagc acaatctaaa tatggacaaa ctgtaagtaa agcaaattta
    12721 caaccaggag acttagtatt cttcacaact aatggttctg gttctgttag tcatgttgga
    12781 atatatgtag gtgggggtaa tatgatacac tcaccatcaa caggaaaaac agttagtgta
    12841 actagtataa attcatcata ctatacagca agatttgtta cagcaaaaag aatattataa
    12901 tcggtttgta atatgacaaa ttgataaata cataaatata tataaacgct ctatattata
    12961 tttaatttaa tcatggaaac aaaataatga ttatatttta aatgtactat agagcgtttt
    13021 tgtatcactt taaatctaat caagattcaa gttaatatga ctgttaaata ggagcctttt
    13081 catatgatga ttatttgtgc aatcccttga caggataggg taaaggtggt aagctactaa
    13141 cagtacaaaa atacttttaa ttttagttca gagaggctaa aaaaggagga cgaaagatgt
    13201 taaaatcaag aaatttaatc caaccagaag acttttcaat agaagaaata gatgaaatat
    13261 tagaattagc tcaaaagata attgacaatc cgtcaaaata ttctcgtata tgtgaaggaa
    13321 aactattagc tacattattt tatgaaccat caacaagaac aagattaagc tttgaatctg
    13381 ctatgaacag attaggagga cgtgtagtag gcttttcaga gccaaattca tcctctgcat
    13441 caaaaggtga aactctagga gatacaatga ggatagtatc tggttatgtt gatataatag
    13501 ctatgagaca tcctcaatca ggtgcagctt cagaagcagc tagatatacg gaagtgccat
    13561 ttataaatgc aggagatggc aaaaaccagc atccaactca aacacttaca gacttactta
    13621 ctataaaatc attaaaagga actttagaaa gccatacaat aggattatgt ggtgatttaa
    13681 aatatggaag aactgtgcat tctttggtta aggctatggc aagatataaa aatacaaagt
    13741 ttgtatttat agcgcctgaa gagttaaaaa tgccagatta tataaaagaa gctataaagg
    13801 gacatgcata ttatgaaact aataatttgg atgatgttat aggaagcttg gatgttttat
    13861 atatgactag agttcaacaa gaaagatttg aagataaatc agagtatgaa agacttaaaa
    13921 actattatat tttaaataaa gctaaattag aaaaggcatc taaaaatatg ttagtaatgc
    13981 atccattacc aagagtaaat gaaatagata tagatgtaga tagtgatgat agagctgtat
    14041 attttaaaca agctaaatat ggaatgtatg ttagaatggc tttaataata aaacttttag
    14101 gtataagtga agactaatag ttatcatata atagtaaaac aaaggagatt ttgggatgta
    14161 taaaattctg gaaaatatat atataggcga agatatgtat agaatgaaag taaaaggtaa
    14221 ttttgaaggt aaaatgggac aattctatat gctaagagca tgggatactt atccagtact
    14281 ttcaagacct ataagtatac atgatataga tgaagaaggg ataacttttc tttataaagt
    14341 agtaggagaa ggaactcaaa ttttgagtaa cctaaaggta aatgatacta taaaattaga
    14401 aggcccttat ggtaatggat atgctaaggt tgatggaaag gtagctctag taggaggagg
    14461 aatcggtgta gctccactat atttggttgc taaaaatata aaaaattgtg acgcatatct
    14521 tgggtttaga gaagatgtta tattagaaga tgaatataag caagtatgta ataaagtcta
    14581 tacaacagta ggaaatactt ttgtaacaga tataattgat gttgagaaat atgattatat
    14641 attaacatgt ggtccaactc taatgatgga aaaattagtt aagatggtag aaggtactaa
    14701 aacgagaatt atggtatctc ttgaaaacca tatggcttgt ggagtaggtg cctgtttagt
    14761 gtgtacgtgt aaaactaatg aaggaaataa aaagacttgt aaagatggtc cagtgttctg
    14821 gggagaggac gtgattttta atgggtaatt taagtgtgaa atttggaagt gtagaattta
    14881 aaaatccagt aataatggcg tctggaactt ttggatttgg aaaagaatat aatgagattt
    14941 atgatataca aaaacttggt ggtataagta gcaaaggtct tactttaaat aaaaaacctg
    15001 gtaacaatgg tatgagagtt catgagacac catctggaat gatgaatagt gttgggcttg
    15061 aaaaccctgg agttcaagga tttatagata atgaactacc tttttttagt aaactagact
    15121 tagtcagaat agctaatgtt ggaggtggga cattagaaga ctacttatta ggggttcaaa
    15181 tgttaaatga taaacctata gacattattg aattaaatat atcttgccca aatgtcaaag
    15241 ctggtggaat ggcttttggt ataaaaaatg aagtagcaag agaagttgtt cgtgaggtaa
    15301 gaaacataac gaaactgcct ttagtaataa aattatcacc taatgcagaa gacattgtag
    15361 gaatggcaaa agtttgtgaa gaagaaggcg ctgatggagt ttcattagtt aatactttta
    15421 aagcaatggc aattgatata aaaaatagaa gaccagtatt tgaaaatgta tatgctgggt
    15481 tatctggacc agctataaaa cctatagctt taagaatggt acatgaagtg tgtaaaaatg
    15541 taaatatacc tgtaatggga atgggaggaa taactaaagc tacagatgct atagaattta
    15601 taatggcagg agctacttgt atccaagttg gaactgcaaa ctttatgaat cctagaatag
    15661 gtattgaaat aatagatgga attaatgaat ttatggatag agaaggaata aaaagtttag
    15721 acgaaataat aggaattata taaggcagaa taaatagtat tttaaagata aaaaatatag
    15781 attaaataaa caataatttt ataacattaa catggaggaa aaataatatg agtaaagtag
    15841 atgtagtaga tatattaaag aaaagtgatg cattgttgga gggacatttt ttattatctt
    15901 caggaaaaca cagtaataga tatgtacaat gtgcaaaagt attaagattc ccagaatatg
    15961 ctgctgaggt attaagtaca gttgttgaac aaataaaaga cttagatata gacttagtag
    16021 taggaccagc tatgggcgga gtaatagttt cttatgagtt aggaagacaa ttgggaaaag
    16081 aagctgtatt tactgagaga aaagacaata caatggagtt aagaagagga tttgaagtta
    16141 aaaaaggagc aaagataata attgctgaag acgttgtaac tactggtaaa tcaactatgg
    16201 agacaaaaag agtattagaa gccttaggtg gagaagttgt aggtgttgca tgtatagcag
    16261 atagaactaa tcatgatata ggtatgccta tatatagtgc tataaaactt gatattcaag
    16321 tttatgaatc tgatgagtgt cctttatgta aggaaggaaa attaccagtt gttaagcctg
    16381 gaagtagaga gttcaaggaa ttaggaatgt aataaaaact taattattca tagtgttact
    16441 taaaaaaggt aaatttttta gagtaaaaac ttgtaatata acatataact taaaaataaa
    16501 aagcagttag ccttataaat gaaggttagc tgtttttttt gttaaaaata tatttacaaa
    16561 ataagagata gtagcagtac aatataagta taaggtaaaa agtagtatat attagggagg
    16621 ggtaagtatg aaaaatcaat taaatgaaaa tcataaaaag gttgtatttt ttggagtagg
    16681 cgctgtagga gctacttttg cagaacaatt ttttaactct aaatatgatt ttaaaattct
    16741 ttgtgataat gaaagaaaga aaagatattt agaagaagga tttataataa atggaaaaag
    16801 gtatgatttt gattatgtaa ctaaagatga gtataagcaa gaggctgatt ttataattat
    16861 aggtttaaaa tataataatt taaaagaaaa tataaaagaa ttagatggat tagttggaaa
    16921 aaatacagtt ataatgtctc tgctaaatgg agttgatagc gaagagataa taggagaaag
    16981 atttggaatt gaaaaaatgg tatattcata tgttaccaat atagatgcaa agaaaatcaa
    17041 taataatatt atacacacta ctaatggggt aattgtattt ggtaacaaag ataatagtga
    17101 agatagaaaa actaatataa taaccgaaat ctttgatgat gtaaatatag aatatatttt
    17161 atcaaaagat attcaacgag atatgtggtg gaagtacatg gttaatattg gtgtaaatca
    17221 aacttcagct atacttggtg caccttatgg agtttttcag agttctgagc atttaagaga
    17281 attagcaaaa tctgcaatga gggaagttgt tgctatagca caagcaaaag acatatctct
    17341 tacagaagat gatgtggaac attcattaca tagaatacta gaacattcaa aagaaggaag
    17401 aacatcaatg cttcaagatg tggaagctca tagacctaca gaagtagata tgttttctaa
    17461 gaatatctgc aaacttggaa aaaaatataa catacctact cctataaatc agactttctt
    17521 ttatatgata aaagtaattg aaagtagatt ttaaagggtt tttgtaagaa atattgataa
    17581 ttattgattt taaattttat taatgttata tactatttaa gttactaaat atttttaact
    17641 cttcaatgaa aatagttgct gcttttgata aagttatatc ttttggaaat atatatgaga
    17701 aatgtctgtt gtagctgctt ccaagctcta tgacagacag ctcattgttt aaaacaggta
    17761 agcttgtcac tagattagag actattgtaa ttcctaaatt atttctaaca gattctttga
    17821 cagcataatt acttccaaaa accatcatac tttttggaat aatttcttta acacttaaaa
    17881 acatatctaa ataatctctg gttccagaac catcctccct gactatccat ttttggtttt
    17941 gaagtttatc aaaactaaaa tcttttaata aatgactatt gtatggaagt gctaaaacca
    18001 tcttatcttc aaaaaaatat tcttgaataa atgaagggga agagcaagtg ccttcaataa
    18061 gaccaatatc taaaatataa tccttaacat gagaagatac aattgatgta ttttttataa
    18121 aaacttctac atctatgtca ggatactttt tagaaaataa tgctagaaaa tttggtagaa
    18181 tatactctcc aatagttaaa cttgcaccaa tctttaagtg accagttata gcatctgaaa
    18241 cttggcttac atcatgataa gtaatattta aaagatttaa tatttcttta gctcttttgt
    18301 acaagatttg tccactttct gttataaaaa tggttttttg tttaatagac ctgtttataa
    18361 gcacaacttt aaaataattt tctagatttt ttatgtgttt acttacactt ggttgtgata
    18421 gatttaagta ttccccagct ttagtaaagt ttttgtattc aacaacagct acaaatgttt
    18481 ttaattcttc aaacaatttt ttgtctcctt tatctaatga aattttacat tttttatata
    18541 ctttctacag ataattatac tatatataat aaccattaaa aacattaatc attacgatat
    18601 tatatattta ttttacttat ggttattaca attatatact attgctatga agaaaactat
    18661 attaattata aggtaggtat attatgttaa agaaaaaaag cagtttgaca ttaaaaacta
    18721 taagggaaat attaccagga ctgtttgtat cagtattagt aggttatatt agtatattta
    18781 tttctattct aattcctaag gtaggggcag catcaatttc tatattttta ggtatgtttg
    18841 taggaaacct atttttaaat caaaaggtat ttcaaaaagg ttataaattt tcagagacag
    18901 atttgttatc atattcaata gtactattag gagcaacact cagtgtatct accttaatag
    18961 acttaaaagt ttcaggaatt ttatttataa tattgcaaat gacaataaca ataatagctg
    19021 cactttatat aggtaaaaaa ctaggatttg aagaaaattt tagatttcta atggcaagtg
    19081 gaaatgcagt atgtggttct tctgctatag gagcaacagt tccagtagtt aatgcgacag
    19141 ataaagaaaa gggaattgca gtaactattg taaatgttac tggtatattc cttatgtttt
    19201 tacttccaat aatatctcaa tttttatata gccatgaact tgtaaaaacg tcagcaatga
    19261 taggtgggac tttgcaatca attggtcagg ttgtagcaag tggagcaatt gttggagaaa
    19321 atgtaaaaga tttggctact atttttaaga tagtaagagt aatatttcta gttgttgttg
    19381 tattagtatt tggacatatg aaaaatcagt ctaataggga aatccttgaa gaagaaaagg
    19441 atgagataaa aaagaaaaaa gtaactatac catggtatgt aattggattc tttataactt
    19501 gtgcactttt ttcaatgaat attataccaa aagaagtatc tgtgttgtgc aaagaaataa
    19561 gtaacaaact agaaataata gctttagcag caattggatt aaaggttaat gtaaaagatt
    19621 tagtgaaaca agggaaagaa gtttctttat atggtttatt tgtaggtaca gtacaagttg
    19681 tatcagctgt agttttaata aaaatattta tatagcataa aaatagtatt tgtaatatta
    19741 atttgattaa ttaaaataat atgatactaa taatttataa tataaataaa aaat
//
LOCUS       DAEPXF010000067        19579 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain HB27
            SAMN13376389-rid12479423.denovo.071, whole genome shotgun sequence.
ACCESSION   DAEPXF010000067 DAEPXF010000000
VERSION     DAEPXF010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13376389
            Sequence Read Archive: SRR10518432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 19579)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19579)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001069624.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001069624.1
            Genome Coverage       :: 315x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/15/2021 01:54:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,165
            CDSs (total)                      :: 4,076
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 89
            rRNAs                             :: 10, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 10 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 19 of 69
            Pseudo Genes (incomplete)         :: 44 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 10 of 69
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19579
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13376389-rid12479423.denovo.071"
                     /strain="HB27"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="China: Hebei"
                     /collection_date="2017"
     gene            50..205
                     /locus_tag="KRL67_002015"
     CDS             50..205
                     /locus_tag="KRL67_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH1522894.1"
                     /translation="MKGILKELTAKLYSFFYFMFWGFYFSAGYFFYKNICTSFLVRPY
                     FSDFEHK"
     gene            335..1348
                     /gene="aroF"
                     /locus_tag="KRL67_002016"
     CDS             335..1348
                     /gene="aroF"
                     /locus_tag="KRL67_002016"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003452924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HBH1522895.1"
                     /translation="MIVVLKMGADKNEVKKLIEAIGREGVEVNPIDGTELTVLGLVGD
                     TSKIDAKRIEANKIVEKVMHVVEPFKKANRKFHPEPSIINVNGMEIGSKKIAMIAGPC
                     SVETEDQIVSIAKDVKKSGAGFLRGGAFKPRTSPYAFQGLKYDGLDLLKKAKEKTGLP
                     IVTEIMSTQDIDIFEENVDVIQVGARNMQNFDLLKELGKTNKTILLKRGLSATIEEWL
                     MSAEYIMAGGNENVVLCERGIRTFETYTRNTLDLSAILAVKKLSHLPVIVDPSHAAGK
                     SWMVDSLSKAAIAVGADGLIIEVHNDPAHALCDGKQSIKPNEYDELISELKTIASAVG
                     REI"
     gene            1370..2434
                     /locus_tag="KRL67_002017"
     CDS             1370..2434
                     /locus_tag="KRL67_002017"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HBH1522896.1"
                     /translation="MGEKLLVNLKENSYNIFIKKNILKNIGEEIKKIYFGNKIFIVTD
                     ENIAKLYLDKVKNNLSESGFEIKSLILKSGEETKSFNTLPKIYDSLLDFKLTRKDLII
                     TLGGGVIGDLGGFAAATFLRGISFIQIPTSLLAQVDSSVGGKVGVDLVRGKNLVGSFY
                     QPKAVFIDPNVLLSLPEKFYRDGMAEVIKYGCIKDKTFFNKLLSLKDREEVMQHIEYI
                     IHKCCFIKKTVVEADEKDFGDRMLLNFGHTLGHAIEKYYNFTGYTHGEAVAIGMYKIT
                     LESEKIGITKKGTSELIKSILMNYNLPYGIELRDNKSIVDAISLDKKNLGDTLKIILL
                     KDIGESIIYNTTSKFFQEVR"
     gene            2434..3747
                     /gene="aroA"
                     /locus_tag="KRL67_002018"
     CDS             2434..3747
                     /gene="aroA"
                     /locus_tag="KRL67_002018"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HBH1522897.1"
                     /translation="MGSLKIYPSKLSGDVKIPPSKSMAHRAVICSSLSNGKSRISNID
                     FSDDIIATIRAMTSLGAIIEKREDILEISGIFSKEGILNRENQLNQPKLTIDCNESGS
                     TLRFLVPISLAFDGVKRFIGRGNLGKRPLDTYYEIFDRQNIKYSYKENQLDLIISGKL
                     KPDEFRVKGNISSQFITGLLFILPTLESDSKIIITTELESKGYLDLTLSTIKDFGVEI
                     INNNYKEFIIKGNQTYKARDYKVEGDYSQGAFYLSADAIGEDISILDLKEDSLQGDSE
                     VVEILSRMGMEILREGNKIKGITNGLNSTLIDASQCPDIIPVLSVVASLSIGKTTIIN
                     AGRLRIKECDRLHAINVELSKLGANIEEKEDSLIIEGVSKLNGGVEVWSHKDHRIAMT
                     LAIASCRCDKPIILKDFECVSKSYPHFFKDFKMLGGRIDEWNMGK"
     gene            3725..4804
                     /gene="aroC"
                     /locus_tag="KRL67_002019"
     CDS             3725..4804
                     /gene="aroC"
                     /locus_tag="KRL67_002019"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HBH1522898.1"
                     /translation="MSGIWGNNLKVSIFGESHGNAIGINIDGLPSGIELDLDKIDKEM
                     KRRAPGKNSISTSRNESDIPEILSGYFNGKTTGTPLCAIIRNSDTRSKDYGELKNLMR
                     PGHADFTGNVRYSGFNDYRGGGHFSGRITAPLVFCGAICKQILSQKGIEIGAHIKKIK
                     NIEDMSFDYVNISKQELSNLQTLELPLLDLSKEEAMKNTIIDAKNQGDSVGGIVECAV
                     VGINVGLGNPFFDSVESTLSHLLFSVPAVKGVEFGLGFELADMYGSQSNDEMYYEGKQ
                     VKSKTNNNGGIIGGITTGMPIIFKVAIKPTPSINRQQNTVNIKDKKDDILSIKGRHDP
                     CIVQRAIPVIEAVTAIGIFDLMKGR"
     gene            4807..6003
                     /gene="pheA"
                     /locus_tag="KRL67_002020"
     CDS             4807..6003
                     /gene="pheA"
                     /locus_tag="KRL67_002020"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HBH1522899.1"
                     /translation="MEDLTRYRNEIDNIDKEIIQLFEKRMNTVLEIARHKMKNNTTIL
                     QKDREEKVLSRAVDNLDNKAYSQETIQFFNSIMEISRGLQKRLMDNGSEQKIEFCLDS
                     SNKKFDLSNVNKYKSLKYELNKKNILVGFPGKSGSFTEEALNKFFNKKISKKQFKEFE
                     DVFIALKNKEIDYGIIPIENSSTGAISETYDLLRKYGFYIVGEECIKIDQNLIGIKDT
                     KLEDIKEIYSHPQGIGQCSEFLKQNSTWKLIPFNNTATSAELVKKLQDKTKAAIASKK
                     AANIYGLEIISPCINDITNNYTRFVVISNQIHIEEESNKMSVVFSVEHEAGKLYKVLG
                     YFAENNINMTKIESRPMKNASWRYFFYIDFECSIYNSKVYDLLELIEHNTAYFKFMGV
                     YKNKTE"
     gene            6050..6859
                     /gene="aroE"
                     /locus_tag="KRL67_002021"
     CDS             6050..6859
                     /gene="aroE"
                     /locus_tag="KRL67_002021"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HBH1522900.1"
                     /translation="MNFFGLVGEKLSHSVSPQIHKRVFEILNIESAYKNFEISKEDIS
                     KLDSAIKLLGIQGVNVTVPYKERIMKYLDFISPEAKRIGAVNTILLRENMLYGYNTDY
                     FGLDSMFKMASIDVQGKVAVILGTGGASKAALTYFIDSGIEKLYVSTRNKDDKKLLNS
                     KAILIDYEELKCIKGDIILNATPVGMYPNVGISPVSKSIIQNFDTLIDLIYNPGETEF
                     LRIGNSMGKKTCDGLYMLVGQAIKSQEIWQDIKIDNSILDAIYNELKLEFL"
     gene            6871..7404
                     /locus_tag="KRL67_002022"
     CDS             6871..7404
                     /locus_tag="KRL67_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HBH1522901.1"
                     /translation="MINKTKEKLILIGMPGSGKTTIGKLLAKEYNCSFCDMDDYIIQI
                     SQKSIAELFSEGEDIFRNYETQACRELSISDKTVISTGGGVIKKDVNMEILKETGIII
                     FIDRPIQKILEDININSRPLLKNGKDRLYNLYNERINLYKKFSDIEILNDKSLNNAIY
                     NITNAVSENFKFEFKEK"
     gene            7425..8264
                     /locus_tag="KRL67_002023"
     CDS             7425..8264
                     /locus_tag="KRL67_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HBH1522902.1"
                     /translation="MNIVIVGLGVIGGSFAKALKKAGYENVFGVDVDLETLKKAEKAK
                     IIKKGCTTGKELFKKADLIILSIYPRLVVDFLNNNKNFFKKGTIITDTTGIKETLIND
                     VLQIIPDDIDFIFGHPMAGREKKGIDFASEQVFNGANYIITPTGRNNIKNLELVENLI
                     LEIGFKRVKKLTSQKHDEIIAFTSQLPHVMAVALINSDEEGRDTGKFIGDSYRDLTRI
                     ANMNEDLWSELFLGNRDNLLKVIENFESEVNLVKEAIFNNDKNKLIEYFKKSSIRREA
                     LEK"
     gene            8532..10013
                     /locus_tag="KRL67_002024"
     CDS             8532..10013
                     /locus_tag="KRL67_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional diguanylate
                     cyclase/phosphodiesterase"
                     /protein_id="HBH1522903.1"
                     /translation="MKKNRLIAKNISIAFIVLFFFSVFTFFYVGNINRALEYEINDIM
                     TVTITGWIILSFLFLGIIIYILYSKAKSQKTIEKVAYTDFVTGYSNWRKFELDVTNLL
                     KKTSQNNKYAMVIFDIDKFKAINDIYGHKKGNLILKDIADTLNELTNINETFARVSAD
                     NFNILLTYNKKEDIINIIKKIMANNELVNLSFGIYEIKDKDLSVSVYSDRASLAKSSI
                     KNNSDVNFAFFNDKLREKLLLEDKIEKEMEYALESGQFVMYLQPKYNIKLDKFCGSEA
                     LVRWQYTEKEVIYPGDFIPIFEKNGFIRKIDMYILEQACKEIRSLFDKGISPLPISVN
                     FSRVDFLKKDFIANIVNICDRYKIPYSLIEIEITESSMFGDTDTLFNVSRNLQDIGFI
                     VAMDDFGSGYSSVNMLKNIPLNVIKLDRGFFVDDKDVDKSQIVIKSIVSLIKQLGIRV
                     VAEGIETRSQIEMLKKANCDIVQGYYFSKPLPIKEFEKLVYKI"
     gene            10046..13483
                     /locus_tag="KRL67_002025"
     CDS             10046..13483
                     /locus_tag="KRL67_002025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HBH1522904.1"
                     /translation="MIRAKSLKNVLSIVLIFLFTIISFSDFAFKTNVQEEENSKINLR
                     EISLQSSNAIKIKLDSSLSFIKSVANMVSYFNTIDNSKIVPLLANISKESDFQRISIV
                     SPDGISHTSDGKIINVSNYQYFKKAIKGESNISEVVKSPIDGKDTFVIATPVFNKNNK
                     IIGVLYGSYYNDKLAEFIDITSFNSEGYIDIFESSGQFVLKSSHTNKLIKSSSNIYDI
                     FKKASFDDGYSYKKIYEDTKNNKSGFMSYEYKGENRYATYTPIGVNDWYVISVIPQYT
                     VRRNIDKINQLAFSLIFKVFLLFIILIIYIIYKNNKSEKDILKANKEISSITNSIPGG
                     VQKCTLGETCNINFLSDGFIELFGYTREEIKTLFNNDFYSLIHKDDVKRIKKEISKQL
                     YKNKTIELEYRIITKNGNTVWVLDKGELITEPDGEEYFCCVTVDITRTKQLEQEMEQK
                     NLELEMISENVLGGIIITEFNDDFTIKYTNAGYLSMIGYTREQLEKELKYKACNLIFY
                     EDRKKALESIQHQLNTKGIAVYQHRIRRRDGNIIWVSIKGRLIIDDDGIEKGIWILND
                     ITDLKNAEQKLKINEERFRIALKQTSNTIFEYDIKTKSLLNTSKELDKYNIYKITKNV
                     PYSLVESGVIHKDFSDDFINMYENVINGAKKASCVVKMKLPDENYIWNKVTLTTIFDE
                     DGNPVRAIGIQEDISKQKEAEIRYNQEGKYHSAILSEAMITYEINVSKDTFIKGNENW
                     QKPFGIKPDNNYSQMVKIIANSIVHKDNKEIFLNTFLCKKLLDAYKNNVTEIKLEYRR
                     MNQENKMIWVICTMHLIKDSITNDIKALAYVKDIDKQKNEEIRLKLKAERDTLTGLYN
                     KGTTEKLINDFLNSEEASLGMHAFLIVDIDNFKSVNDNLGHVFGDKVLFEVSKHLKPI
                     FRKDDIVGRIGGDEFIVFLKNIHSFENTKKKAEKICDIFRNSYTENKKTYKISGTVGI
                     SFVPEHGTVFEELYKKADIALYYAKSKGKDKYALYNDCIHQNELENNISDNNIKLADK
                     YEESKPVLKEILDSIDNYIYIVNQNTYELLFVNNKVSRLDLDIKLGETCYKSLLKFDR
                     PCNNCPLNGLEENKHSKFKIETNTFGKNISIASWINCIDGEYNCLIECIDSSLYKNK"
     gene            13501..14247
                     /locus_tag="KRL67_002026"
     CDS             13501..14247
                     /locus_tag="KRL67_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HBH1522905.1"
                     /translation="MLNSITLILGIIFTTYYFYLKIVFGGISFSEIFLVVGIVLIIYQ
                     IFKKKIKEKKVFYRVLKIIISIFLIVFVATESLIIFYPKNSLESKSDYLLILGASVKK
                     TTLSTTLKGRLDTALKYLKIDDNCYVVVSGGKGSGEKITEAKAMKDYLIKNGIDKNRI
                     IEEDKSTNTYENFKYSKLKIEEHNERKLSDLKVKIVTTDFHVLRSKILAYRNGYKNTS
                     FYASKSKLSFVPTYYTREFFALWKTIVFDR"
     gene            complement(14196..14516)
                     /locus_tag="KRL67_002027"
                     /pseudo
     CDS             complement(14196..14516)
                     /locus_tag="KRL67_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889798.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
     gene            15305..19444
                     /locus_tag="KRL67_002028"
     CDS             15305..19444
                     /locus_tag="KRL67_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF11 domain-containing protein"
                     /protein_id="HBH1522906.1"
                     /translation="MATVSGQIVFDRNRSATIDSGDSGIANVPVVLQDIATGVRLVVL
                     TDASGNYVFTNVPNGNYRIVEAFGTTGGVPTPGNFATAIVGPVPIAEMPPISSVTNPP
                     IGSTNLDGTTPNTLLITVAGSDITNQNILNGPVIYTPIENILDSCVSVSNINLITDAD
                     NGTFGFFPPGTPSNTGPEIEPYPNVTPDFTYVVPDPTKFTPLDGEYTVQNIMTNAMSN
                     VIGAWWRIADHTTGNETGRMMVVNGFNPGAVFFKSTVQATPNTNYLFSTWILNLFKVT
                     GYPPPQLGVRIFDQNNNILYQATLGAQIPVNTNAPEWKEVGTVINSQNNNQLTVEFFS
                     EGESAIGNDYAIDDVSLREIAVPTFTPIKTSNTETANVGDIITYTVTLDNTCDSPLTN
                     VFFRDLIPNGLEFIPGTVTVDEVSVPGVDPNTGFPLPDIGGGTGAEVTFDVVVESIPN
                     PNPTNNIATIDYSYTPVEGGIPNDFSVDSNPVPVEVISADIEVTKLSEPTIVNPGEEL
                     IYTIKVVNNGPFPSENVVLTDDIPASIINPEYSLDGGVTFQPWTGNLNIGTLEVGETR
                     VIIIRGIVNPSTVGIITNTAVVSSTTADPNLNNNTSTIETEVNLLADILVMKTAEPNS
                     AVPGTLLRYTIQVENLGPANAENVILNDDIPSSIINPEYSLDGGASFQPWNGSLNIGT
                     LNSGISLTVLIQGTVSLNSSEYIVNTATVSSPTPDPDLSNNISTIITPVNPQAGISII
                     KVADEDVAVPGEEFVYTIEIFNEGPSNATNVVLTDDIPDVILNPEYSLDGGASFQPWN
                     GSLNIGTVVPGQLIRIIIKGLVSSTATGDITNTAEVSADVPEPVMDSSTVTTPIVASA
                     DIEVIKTSNMDTAVPGETFSYTITVTNLGPSAAQSIVLADDIPNVILNPEYSLDGGVT
                     FQPWNGNLNIGTLDTGEIRSIIIRGTVSQTAVGTIINTATISSPTPDPNPDNNTSTDE
                     TDISPLADISVIKGNEPLAIPGGRFIYGIEIANAGPSFAENVTLTDNIPASILNPEYS
                     IDNGVTFQPWNGSLNIGTLDAGEIKNIIIRGTVSQTAIGIIINTATISSTTPDPNLNN
                     NTSTSEAEVSSSADISVVKRSNQAVVVPGDVLDYTIEARNAGPSTAQNVTLTDNIPVS
                     ILSPEYSIDNGVTFQPWTGSLNIGTLDTGGIRNIIIRGIVSETAVGTIINTATVSSTT
                     PDPNPNNNTSTSEIDISSSADISVIKLANKTEACVGDEIDFVIVVSNAGPENAQNVTL
                     IDNVSDKLKKTVFSLDRGVSFQPWTGSLNIGTLTAGTLRVIILRGIIKSTCLDKLTNI
                     AEVTSTTPDSDLNNNISRVQVEIKQCCCNDCCCNNCSNCWCNCCCKDCYCNCCKNDFW
                     C"
BASE COUNT         7962 a         2161 c         3285 g         6171 t
ORIGIN      
        1 aattattaaa caagaagaat aataaattat aaaaatggag gataatacta tgaaaggaat
       61 attaaaagaa ctaacagcaa aactttatag ctttttttac tttatgttct ggggctttta
      121 ttttagcgct ggctatttct tttacaaaaa tatatgtact tcctttctgg tgaggcctta
      181 ttttagtgat tttgagcata agtaaatttt ataaaaatga ttattttata tatttatata
      241 atatttagtg cttgatagat tattttagag aactcattag tgagttctct tttttatttt
      301 aaatataaaa ttataatatt gggaggtagt aaaaatgata gtagtattaa aaatgggagc
      361 agataaaaat gaagttaaaa aattaattga ggctattggg agagaaggag tagaagttaa
      421 tcccatagat ggaacagaat taactgtttt aggtttagtt ggagatacta gtaaaataga
      481 tgccaaaaga atagaagcaa ataagatagt tgaaaaagtt atgcatgtag tagaaccttt
      541 taaaaaggcc aatagaaaat ttcatccaga accttcaatc ataaatgtta atggaatgga
      601 aattggttca aagaaaattg ctatgatagc tgggccttgt tctgttgaaa cagaagacca
      661 aatagtatct atagctaaag atgtgaaaaa atcaggtgca ggatttttga gaggtggagc
      721 attcaaacca agaacatcac catacgcttt ccaaggattg aaatatgatg gattggattt
      781 attaaaaaaa gcaaaagaaa aaacagggct tcctatagtt acagaaataa tgtcaacaca
      841 agatatagat atatttgaag aaaatgtaga tgtaattcaa gttggagcta gaaatatgca
      901 aaacttcgat ttattaaaag aattaggtaa aacaaataaa acaatacttt taaaaagagg
      961 attatctgct acaattgaag agtggctaat gtcagcagag tatataatgg ctggaggaaa
     1021 tgaaaatgta gttctttgtg aaagaggtat aagaactttt gaaacatata ctagaaatac
     1081 tttggattta agtgcaattt tagctgtgaa gaaacttagt catttaccag ttatagttga
     1141 cccaagtcat gcagcaggta aatcatggat ggttgactct ttatctaagg cagcaattgc
     1201 agtcggagca gatggtctaa taatagaagt tcataatgac ccagcacatg cactttgtga
     1261 tggaaaacaa tcgattaagc caaatgaata tgatgaattg ataagtgaat taaagactat
     1321 cgcatcagca gttggcagag aaatataatc tataaaagga tggttaaacg tgggagaaaa
     1381 actattagta aatttaaaag aaaatagcta caatatattt attaaaaaaa atatactaaa
     1441 gaatatagga gaagaaataa aaaaaatata ttttggaaat aagattttta ttgtaactga
     1501 tgaaaatata gcaaaattat acttggataa agtaaaaaat aatttaagtg aatctggatt
     1561 tgaaattaaa tctttaatat taaaatctgg tgaagaaaca aaatcattta atactcttcc
     1621 taaaatatac gatagtttac ttgactttaa gttaactaga aaagatttga ttattacttt
     1681 aggaggaggg gtaataggtg atttaggtgg atttgcagca gcaacttttt taagaggtat
     1741 aagttttatt caaattccaa catctttatt agcacaagtt gatagttctg taggtggaaa
     1801 agttggagtt gaccttgtga gaggtaaaaa tttagtggga agcttttacc aaccaaaggc
     1861 tgtgtttatc gacccaaatg tattattatc acttccagaa aaattttata gagatggaat
     1921 ggcagaagtt ataaaatatg gatgtataaa agacaaaact ttttttaata aacttttaag
     1981 tttaaaagat agagaagaag taatgcaaca tatagaatac attatacata aatgttgctt
     2041 tattaaaaaa actgtagttg aagctgatga aaaagacttt ggagatagaa tgcttctaaa
     2101 ctttggtcat acattaggtc atgctattga gaaatactac aattttacgg gttataccca
     2161 tggagaagct gttgccatag gaatgtataa gataacttta gaaagtgaaa agataggaat
     2221 tacaaaaaaa ggtacatcag aacttataaa atcaatttta atgaactata atttgcctta
     2281 tgggattgaa ttaagagata ataaatctat agtagatgct atatctcttg ataaaaaaaa
     2341 tctaggtgat acattaaaaa taatactttt aaaagatatt ggagagagta ttatatataa
     2401 tactacttct aaattttttc aggaggtaag ataatgggaa gtctaaaaat atatccaagt
     2461 aaattatctg gagatgtaaa aataccacca tcaaaaagta tggcacatag agcagttatt
     2521 tgttcatctc taagcaatgg aaaaagtaga atttctaata tagatttttc tgatgatata
     2581 atagctacta taagagcaat gacatcatta ggagctatta tagaaaagag agaagatatt
     2641 ttagaaattt cgggaatttt ttctaaagaa ggtattttaa atagagagaa tcagttgaat
     2701 caacctaaat tgacaataga ttgtaatgaa tcaggctcta cattgagatt tttagtacct
     2761 atatctttag catttgatgg tgtaaaaagg tttataggta gaggtaatct tggaaaaaga
     2821 ccacttgata cttattatga gatatttgac agacagaata taaaatatag ctacaaagaa
     2881 aatcagttag atttgattat atcaggtaag cttaaaccag atgaatttag agtaaaagga
     2941 aatataagtt ctcagtttat cacaggtcta ttgtttatat tgcctacatt agagagtgat
     3001 tcaaagataa taattactac tgagctagaa tctaaaggat atttagattt aactctttca
     3061 acgataaaag attttggggt agaaattata aacaataact ataaagaatt tattataaaa
     3121 ggaaatcaaa cgtataaagc gagagattat aaagtagaag gagattattc tcaaggagca
     3181 ttttatttat cagcagatgc tataggagaa gatattagca tattagacct taaagaagat
     3241 tctcttcaag gggatagtga agtagttgaa attttatcta ggatgggaat ggaaatatta
     3301 agagaaggca acaaaataaa aggaataaca aatggtttaa atagtacttt gatagatgca
     3361 tcacaatgtc cagatataat acctgtatta tctgtagtag catccttaag tatagggaag
     3421 actacaataa ttaatgctgg taggcttaga attaaagaat gtgatagact tcatgcaata
     3481 aatgttgagt taagtaaact tggagcaaat atagaggaga aagaggatag tttgataatc
     3541 gaaggagtaa gcaagttaaa tggtggagta gaagtgtgga gtcacaaaga ccatagaatt
     3601 gctatgacac ttgcaattgc ttcttgtaga tgtgataaac caataatatt aaaagatttt
     3661 gagtgtgtat caaaatctta tccacatttt tttaaagatt ttaagatgtt aggaggtcga
     3721 attgatgagt ggaatatggg gaaataattt aaaagtatca atttttggag aatctcatgg
     3781 aaatgctata ggtataaata tagatggtct tccttcggga attgaacttg accttgataa
     3841 gattgataaa gaaatgaaga gaagagcacc tggaaaaaat tctatatcta cttcaagaaa
     3901 tgaaagtgat ataccagaaa ttctaagtgg atattttaat ggaaaaacta caggaacgcc
     3961 attatgtgca atcataagaa atagtgatac tcgttcaaaa gattatggag agcttaaaaa
     4021 tttaatgaga ccaggacatg cagatttcac aggtaatgtg agatattctg gatttaatga
     4081 ctatagaggg ggaggtcatt tttcaggtag aataacagct ccattagtat tttgtggggc
     4141 tatatgtaaa caaattttat cacaaaaagg aatagagata ggagcacaca taaaaaaaat
     4201 taaaaatata gaagatatga gttttgatta tgtaaatatt tcaaaacaag agttatcaaa
     4261 tttgcaaact ttggaactac ctttgcttga tttatcaaag gaagaagcca tgaaaaatac
     4321 gattatagat gcaaaaaatc aaggagattc agttggtggt atagttgagt gtgcagtagt
     4381 aggtattaat gtagggcttg gaaatccatt ttttgactct gttgaatcta ccttatcaca
     4441 tttattgttt tctgttcctg ctgtaaaagg tgtagagttt ggcttaggtt ttgaacttgc
     4501 agatatgtat ggttctcaaa gtaatgatga aatgtattat gaaggaaagc aagttaagag
     4561 caaaacaaat aacaatggag ggattatagg agggattact actggaatgc caataatttt
     4621 caaggtggca attaagccaa ctccatcaat taacagacaa caaaacacag ttaatataaa
     4681 agataaaaag gacgatattt tatctattaa aggcagacat gacccttgta tagttcaaag
     4741 ggctatacct gtaatagaag cagtaacggc cattggaatt tttgatttga tgaaaggacg
     4801 ataataatgg aggatttaac gagatataga aatgagattg acaatataga taaagagatt
     4861 attcaattat ttgaaaagag aatgaataca gttcttgaaa ttgccagaca taaaatgaaa
     4921 aataacacta ctatacttca aaaagataga gaagaaaaag tactaagtag agccgtagat
     4981 aatttagata ataaagcata ttcacaagaa acgatacagt tttttaattc tataatggaa
     5041 attagtagag gattacaaaa aagattaatg gataatggta gtgagcagaa aatcgagttt
     5101 tgtttagatt catcaaataa gaaatttgat ttgtcaaatg taaataaata taagtcttta
     5161 aaatatgaat taaataaaaa aaatatccta gtaggttttc caggaaaatc aggttcattt
     5221 acagaagaag cactaaataa attcttcaac aaaaaaatat caaaaaaaca atttaaagaa
     5281 tttgaagatg ttttcatagc acttaaaaat aaagaaatag actatggaat aatacccata
     5341 gaaaattcat ctacaggagc tatctctgaa acttatgacc ttttaagaaa gtatggtttt
     5401 tatatagttg gagaagaatg cataaaaata gaccaaaatt taatagggat aaaagataca
     5461 aaacttgaag atataaaaga aatatattct cacccacaag gtataggaca atgtagtgaa
     5521 tttttaaaac aaaatagtac atggaaattg ataccattta ataatacagc aacaagtgcc
     5581 gaacttgtaa aaaaacttca agataaaaca aaagcagcaa ttgcaagcaa aaaagctgca
     5641 aatatctatg gattagaaat aataagtcct tgtataaatg atattacaaa caactataca
     5701 agatttgtag taattagtaa tcaaatacac attgaagaag aatcaaacaa aatgagtgta
     5761 gtattttctg ttgaacatga agcaggaaaa ctttataaag tacttggata ctttgctgaa
     5821 aataatataa atatgacaaa aatagaatca agacctatga aaaatgcctc atggagatat
     5881 ttcttttata tagattttga gtgttctata tacaattcta aagtgtacga tttattagaa
     5941 ttgatagaac ataatacagc atattttaaa tttatgggag tatataaaaa taaaactgaa
     6001 tagataagat aaatttttat ttaatttata attattagga ggtataagta tgaatttttt
     6061 cggtctagta ggtgaaaaac tctctcacag tgtttcacct caaatccaca agagagtatt
     6121 cgagatattg aatattgaaa gtgcttataa aaactttgaa atttcaaaag aagatatatc
     6181 taagctagac agtgctataa aattattagg aatacaagga gtaaatgtta ctgtaccata
     6241 taaggagcgt attatgaaat atcttgattt tatatcgcca gaggcaaaac gtataggagc
     6301 agttaataca attttactca gagaaaatat gttatatggt tataatacag attactttgg
     6361 ccttgatagt atgtttaaaa tggctagtat tgatgttcaa ggaaaagtag cagttatatt
     6421 aggaactgga ggtgcttcta aagctgctct tacatatttt atcgattctg gtatagaaaa
     6481 gctttatgtt tcaactagaa ataaagatga taaaaaatta ttaaatagta aagctatatt
     6541 aatagattat gaagaactta aatgtataaa aggagatata atattaaatg ctacaccagt
     6601 aggtatgtac cctaatgtag gaatttcacc agtgtcaaaa tctattatac aaaattttga
     6661 tacattaata gatttaattt acaatcctgg agaaacagag tttttaagaa ttggtaatag
     6721 tatgggcaaa aaaacctgtg atggattata tatgctggta gggcaagcaa taaaatctca
     6781 ggaaatttgg caagatatta aaatagataa tagtatttta gatgctatat ataatgaact
     6841 aaaattagaa tttttgtaag ggagattgtt atgataaata aaacaaaaga aaaattgata
     6901 ttaataggta tgccaggtag tggaaagact acaattggta aacttttggc caaagaatat
     6961 aattgtagtt tctgtgatat ggatgattat ataattcaaa tatctcaaaa aagtatagca
     7021 gagttgtttt cagaaggaga agatattttt aggaactatg agactcaagc atgtagagaa
     7081 ctttctataa gtgataaaac agtgatatct acaggtggag gtgtaataaa aaaggatgtt
     7141 aatatggaaa tattaaaaga aacaggtata ataattttta ttgatagacc tatacaaaag
     7201 attttggaag atataaatat taattcaaga ccattattaa aaaacggaaa agatagattg
     7261 tataatcttt ataatgagag aataaatctt tataaaaaat tttcagatat agagatttta
     7321 aatgataaat cattaaacaa tgctatatat aatattacaa atgcagtcag tgaaaatttt
     7381 aaatttgagt ttaaagaaaa ataatctagg gggaagtaaa aataatgaat attgttattg
     7441 taggactagg agttatagga ggctcttttg ctaaagcatt aaaaaaagct ggatatgaaa
     7501 atgtatttgg agtagatgta gatttagaaa ctttaaaaaa agcagaaaaa gcaaaaatca
     7561 taaaaaaagg atgtacgaca ggtaaagagt tatttaaaaa agctgattta attatactat
     7621 ctatatatcc tagattagtt gtagattttc taaataataa taaaaacttt tttaaaaaag
     7681 gaactataat tactgatact acaggaataa aagaaacact tatcaatgat gtgttgcaaa
     7741 ttattccaga tgatatagat tttatatttg gacatccaat ggcaggtagg gaaaaaaagg
     7801 ggattgattt tgcaagtgaa caagttttta atggcgcaaa ttatataatt actccaactg
     7861 gcagaaacaa cataaaaaat ttagagttag ttgaaaattt aattttagag attggattta
     7921 aaagagtaaa aaagttaact tcacaaaaac atgatgagat tatagctttt acttctcagt
     7981 tacctcatgt tatggcagtt gctcttataa atagtgatga agaaggtaga gatacgggta
     8041 aatttattgg agatagttat agagatttaa ctagaattgc aaatatgaat gaagacttat
     8101 ggagtgaact ttttttagga aatagagata atttactaaa agttatagaa aactttgaat
     8161 cagaagtaaa tttagtaaaa gaagctatat ttaacaatga taaaaataaa ttgatagagt
     8221 attttaaaaa atcatcaata agaagagaag ctttagaaaa ataaatatta atatttaagc
     8281 tatatataga tgggtataaa tataagcgat agtgtattta attgtattaa taaatatgga
     8341 ttcaatataa ttaaatatgc tattgatatg tttgtaccct taagtattgt tatcttttaa
     8401 atgataatga tatttattta aatgctcttt attaacaatt tataaaactt aattgtaaaa
     8461 aatacttatt aatagtaaaa taaagtgaat caattatatt ataattgcta aagagagtga
     8521 ggtgtagcta tttgaaaaaa aatagattaa tagcaaagaa tatatcaata gcatttatag
     8581 tactattctt ttttagtgta tttacattct tttatgtagg taatattaat agagcgttag
     8641 aatatgaaat aaatgacata atgacagtta caattactgg atggattata ctttcatttt
     8701 tatttttagg tattataatt tacatattat attcaaaagc aaagagtcaa aaaactatag
     8761 aaaaagtagc atatacggat tttgtaacag gctattccaa ctggagaaag tttgaattag
     8821 atgttacaaa tttattaaag aaaacatcac aaaataataa atatgcaatg gtcatattcg
     8881 atattgataa atttaaagca attaatgata tatatggcca taaaaaggga aatttaatat
     8941 taaaagatat tgcagatact ttaaatgaat tgactaatat caatgaaact tttgctcgtg
     9001 taagtgctga taattttaat atattattaa catataacaa aaaagaggat ataataaata
     9061 ttatcaaaaa gattatggca aataatgaac ttgtaaactt atcttttgga atttatgaaa
     9121 ttaaagataa ggatttatct gtaagtgtat atagtgatag agcttcactt gcaaaatcat
     9181 caataaaaaa taatagtgat gtaaattttg cattttttaa tgataaatta agagagaaat
     9241 tattacttga ggataagata gaaaaagaaa tggaatatgc tcttgaaagt ggacaattcg
     9301 taatgtattt acaaccaaaa tataatatta aattagataa attttgtgga agtgaagccc
     9361 tagtcagatg gcaatataca gaaaaagagg tcatatatcc aggtgatttt attcctatat
     9421 ttgaaaaaaa tggatttatt agaaaaattg atatgtacat attagaacaa gcttgtaaag
     9481 aaatccggtc tttatttgat aaaggaattt cacctttacc tatatctgta aacttctcaa
     9541 gagttgattt tttaaaaaaa gattttattg caaacatagt aaatatatgt gatagatata
     9601 aaataccata tagtttaata gaaattgaaa ttactgaatc tagtatgttt ggagatactg
     9661 atactctatt taatgttagt agaaacctac aggatattgg ctttatagtt gcaatggatg
     9721 attttggctc aggatattct tctgtaaata tgcttaaaaa tataccatta aatgtaataa
     9781 aattagatag aggatttttt gttgatgata aagatgttga taagagtcaa attgttataa
     9841 aaagcatagt ttctctcatt aagcaattag gaataagggt tgttgctgaa ggaattgaaa
     9901 caagaagtca aatagaaatg ttaaaaaaag caaattgtga tattgttcaa ggatattatt
     9961 tttcaaaacc attaccaatt aaagaatttg aaaaattagt ctataaaata tagtcagcga
    10021 gtcttaaatg ggaggtggtg attaagtgat aagagcaaaa tcgttaaaaa atgtactgag
    10081 tattgtttta atatttttat ttacaataat ttctttttct gattttgcat ttaaaactaa
    10141 tgtccaagaa gaagaaaata gtaaaattaa tttaagggaa atatccctac aaagttccaa
    10201 tgctataaaa ataaaattag atagttctct atcctttatt aaaagtgtgg caaatatggt
    10261 ttcttatttt aatactatag ataactcaaa aatagttcca ttattagcta atatatcaaa
    10321 agaatctgat tttcaaagaa tctctattgt atctcctgat ggaataagtc ataccagcga
    10381 tggcaaaatt atcaatgtgt caaattatca atattttaag aaagctatta aaggagaaag
    10441 taatatatct gaagttgtaa agtctccaat tgatggaaaa gatacttttg ttatagctac
    10501 tcctgtattt aataagaata acaaaataat tggtgtttta tacggttctt actataatga
    10561 taaattagct gaatttatag atataacaag ttttaatagt gaagggtaca tagatatatt
    10621 tgaatctagt ggccaatttg ttttaaaatc ttcacataca aacaagctga ttaagtcaag
    10681 tagtaatata tatgatatat ttaaaaaagc ttcttttgat gatggttata gctataaaaa
    10741 aatatatgaa gatacaaaaa ataacaagag tggctttatg tcatatgaat acaaaggaga
    10801 aaatcgttat gctacttata cacctattgg tgtaaatgac tggtatgtaa tttctgtgat
    10861 accacaatat actgtaagaa gaaacattga taaaataaat caattagcat tttctcttat
    10921 tttcaaggta ttcttattgt ttattatact tataatatat attatttata aaaacaataa
    10981 atctgagaaa gatatactaa aagctaacaa agaaatctca tctattacaa atagcattcc
    11041 tggaggagta caaaagtgta ccctgggtga aacatgcaat attaattttt taagtgatgg
    11101 atttatagag ttgtttggtt atacaagaga agaaataaaa acattgttta ataatgattt
    11161 ttatagttta atacataaag atgatgttaa aagaataaaa aaagaaattt ctaaacaact
    11221 ttacaaaaat aaaactattg aattagaata taggattata acaaaaaatg gtaatacagt
    11281 atgggtttta gataagggag agcttataac agaacctgat ggagaagagt atttttgttg
    11341 tgtaactgta gatattacta gaacaaaaca attagaacaa gagatggaac aaaaaaatct
    11401 agaacttgaa atgatatcag aaaatgtttt aggtggtata attataacag aatttaatga
    11461 tgattttact ataaaataca ctaacgctgg ttatctttca atgattggat atacacgtga
    11521 acaattagaa aaggaactta aatacaaagc ttgtaatctt attttttatg aggatagaaa
    11581 gaaggctctt gaaagtatac aacatcaatt aaacacaaaa ggaatagctg tatatcaaca
    11641 tagaataaga agaagagatg gtaatattat ctgggtttct ataaaaggaa ggcttattat
    11701 tgatgatgat ggtattgaaa aaggtatttg gattctaaat gacataacag atttaaaaaa
    11761 tgcagaacaa aaacttaaaa taaatgaaga aagatttaga atcgcattaa agcaaacatc
    11821 aaacactata tttgagtatg atataaaaac aaagtcactt ttaaatacta gtaaagagct
    11881 tgataaatat aatatttata aaattactaa aaatgttcca tattctctag tagaatcagg
    11941 agttattcat aaagatttta gtgacgattt tataaatatg tatgaaaatg tgataaatgg
    12001 agcaaaaaaa gcaagttgtg tagttaagat gaaacttcca gatgaaaatt atatatggaa
    12061 taaagttacc ttgactacta tttttgatga agatggtaat cctgtaagag caattggtat
    12121 acaggaagat ataagcaaac aaaaggaagc agaaataaga tacaatcaag aaggtaagta
    12181 tcattcagct atattgtcgg aagcaatgat aacttatgaa ataaatgtat caaaggatac
    12241 ttttatcaaa ggaaatgaaa attggcaaaa accatttgga attaagcctg acaataacta
    12301 ttctcaaatg gtaaaaatta tagcaaatag tattgtacat aaagataata aagaaatatt
    12361 tttaaataca tttttatgta aaaaattatt ggatgcatat aaaaataatg taactgaaat
    12421 aaaattagaa tatcgaagaa tgaatcaaga aaataaaatg atttgggtca tttgtacaat
    12481 gcacttaata aaagattcta ttacgaacga tataaaagca cttgcttatg ttaaagatat
    12541 tgacaagcaa aaaaatgaag aaattagatt aaaattaaaa gctgaaagag atactcttac
    12601 aggattatat aataaaggta ctacagaaaa attgattaat gatttcttga actctgagga
    12661 agcaagctta ggaatgcatg catttttaat tgttgatata gataatttta aatctgtgaa
    12721 tgataattta ggacatgtat ttggagataa agtattattt gaagtttcta aacacctaaa
    12781 acctatattt agaaaagatg atatagtagg tcgaattggt ggagatgaat ttatagtatt
    12841 cttaaaaaat attcattctt ttgagaatac caaaaagaaa gcagaaaaaa tatgcgatat
    12901 atttagaaat tcttatacgg aaaataaaaa gacatataaa atatctggaa cagttggaat
    12961 ttcatttgta ccagaacatg gaactgtatt tgaagaactt tataaaaaag cagacatagc
    13021 actttattat gcaaaaagca aaggaaaaga taagtatgca ctttataatg attgtatcca
    13081 ccaaaatgaa cttgaaaata atataagtga taataatata aaattagcag ataaatatga
    13141 ggaatccaaa ccagtactta aagaaattct tgattctatt gataattata tatatatagt
    13201 gaatcaaaat acttatgaac ttctttttgt caataataag gtgtctcgtt tggatttaga
    13261 tataaaatta ggagaaacct gttataaatc actattaaaa tttgatagac catgtaataa
    13321 ttgtcctctt aacggattag aagaaaataa gcattcaaaa ttcaaaatag agacaaatac
    13381 atttggtaaa aatatcagta tagcaagttg gattaattgc atagatggag aatataactg
    13441 tcttatagag tgtatagatt cttctttata caaaaataaa taaaaaagga ctaagaaggt
    13501 atgttaaata gtataacttt gatacttggt ataatattta caacttacta tttttatctc
    13561 aaaatagttt ttggaggaat ttcatttagt gagattttct tagttgtggg aattgtacta
    13621 attatctatc aaatatttaa gaaaaaaatc aaagaaaaga aggtttttta tagagtgtta
    13681 aaaataataa tcagcatttt tttaatagta tttgtagcta ctgagagtct aattattttt
    13741 tatcctaaaa acagtttaga atcaaaaagt gattatctct taattttagg agcttctgta
    13801 aaaaagacta cactaagtac aaccctgaag ggtaggttag acactgcatt gaaataccta
    13861 aaaattgatg ataattgtta tgtggtagta tctggtggta aaggaagtgg agaaaaaata
    13921 actgaggcaa aagctatgaa agattacctt attaaaaatg gcatagataa gaataggata
    13981 atagaagagg ataaatccac taatacttat gagaatttta aatattcaaa attgaagata
    14041 gaagaacata atgaaagaaa actaagtgat ttaaaggtaa aaatagtaac aacagatttt
    14101 catgttctta ggagcaaaat attagcatat agaaatggat ataaaaatac gtctttttat
    14161 gctagtaaat caaaactttc atttgtacca acttactata caagagaatt ttttgcatta
    14221 tggaaaacaa tcgtttttga tagataataa ataaaaagac tagatataaa ctcatatcta
    14281 gtcttttcta ttcccttaat tctaatccat tttccatctt tacttgctgt agaaatttca
    14341 atattgggat ttacaagtaa ttgcttataa gaagttttat gttttccgat tccaaaatat
    14401 aattttcatt cataatccat tacaaatccg aatggtctta ctcttggttt gttgtattca
    14461 actgtagcaa aatagactgt tgagttttct tttaaaaaat tcattatttt tttcatgatt
    14521 aaatcctctt tgtatcttat actaatatca tgaatataaa taaaatattc ataatactaa
    14581 tataaatcga cataaaaaca attacaagat tatattgatt attcaaaatt atatataaaa
    14641 atcagaaaaa cattttagtt tacataataa aaataatgtt aactaaaatt gttgatttta
    14701 gattaaagtg ccagattatg catttaagta gattttaagc ttgatttgat tagaaaggta
    14761 caaactatca ttgaaaaaaa ttaaacccat ttaaaaatta atataaaagc ttgttatttt
    14821 gagaaaaata gactctctaa acttaattaa ataaaaataa aatacagaaa attatatctc
    14881 tgaatatctt aagtaattga gtttaaattt tatgatattt tgtttgatga tttataatat
    14941 aaaaacatat tcttaatctg ctcaaattaa aaagagtaaa aaataacaaa aaaatcactg
    15001 acatttcact gacaaattgt aattttgtta ttataaatac attgaaaata cttgattatt
    15061 gaaaaatggc ataaaataat accttttatg ccatttacta ttatttgata atttttaact
    15121 tatgacataa gatatttttt cacatataca ataaaatata aaaaagttga tattgcaaat
    15181 aaaaaaatac atgttagatg ttatagaaaa aaaacgcact tataggctta aaatgtcaca
    15241 taaaagagtt ttaaagaata atatattatg aacacaattt ttgtaatcac aaaggagtga
    15301 taatatggca acagtatcag gacaaatagt ttttgataga aatagaagtg ctacgattga
    15361 ttctggagac tcaggaatag ccaatgtccc agttgtatta caagatatag caactggagt
    15421 tagacttgtg gttctcacag atgcatcagg aaattatgtt tttactaatg ttccaaatgg
    15481 taactataga attgtagaag cttttggaac tacaggagga gtaccaacac cagggaattt
    15541 tgcaacagcg atagtaggtc cagttccaat tgcagaaatg ccacctatta gttctgtgac
    15601 aaatcctcct attggttcaa caaacttaga tggtacaaca cctaacacac tattaataac
    15661 agtagcaggt tcagatataa caaatcaaaa tatattaaat gggcctgtga tttatacacc
    15721 tatagagaat atattggatt cttgcgtatc tgtatctaat ataaacctta ttacagatgc
    15781 agataacgga acatttgggt tttttccgcc aggtacacca agtaatacag gacctgaaat
    15841 agaaccttac ccaaatgtga cgccagattt tacgtatgtt gtaccagacc caactaagtt
    15901 tacaccttta gatggagaat atacagtaca aaatattatg acaaatgcaa tgagtaatgt
    15961 tataggagct tggtggcgta ttgctgacca tactacagga aatgaaacag gaagaatgat
    16021 ggtggtaaat ggttttaatc ctggagctgt attttttaaa tcaacagtac aagctacacc
    16081 taacactaat tatttattta gcacttggat tttaaacctt tttaaggtaa caggatatcc
    16141 accaccacag ctaggtgtaa gaatatttga ccaaaataat aatatactat atcaagcaac
    16201 attaggagct caaattccag ttaatacaaa cgcaccagaa tggaaagaag taggtactgt
    16261 gattaattct caaaataata atcaacttac agtagaattt tttagtgagg gagaaagcgc
    16321 cattggaaat gactatgcta tagatgatgt ttctttaaga gaaatagctg tcccaacttt
    16381 tactccaata aaaacaagta acacagaaac tgctaatgta ggtgatataa tcacatatac
    16441 agttacatta gataatactt gtgatagccc tttaactaat gtatttttta gggatttaat
    16501 tccaaatggt ttagagttta tacctggaac tgtaacggtt gatgaagtat ctgtaccagg
    16561 agtagaccct aatacaggtt tcccacttcc agatattgga ggaggaacag gggctgaagt
    16621 aacttttgat gttgtggttg aaagtattcc taacccaaat cctactaata atatagctac
    16681 tatagattac tcctatacac ctgtagaagg cggtatacca aatgattttt cagtagattc
    16741 taatccagtt cctgttgaag taatctcagc agatattgaa gttacaaaat tatctgaacc
    16801 tactatagtg aatccaggag aggaattaat atacactata aaagtagtaa ataatggacc
    16861 attcccatct gagaatgtgg ttttaacaga tgatatacca gcttctatta taaatccaga
    16921 gtattcatta gatggaggag taacttttca accatggact ggaaatttaa atatagggac
    16981 attagaagta ggagagacaa gagttattat aattaggggt attgtaaatc catcaacagt
    17041 aggaattata acaaatacag ctgtagtatc ttcaactaca gcagacccaa accttaataa
    17101 taatacatct acaatagaga cagaagtgaa tttattagca gatattttag taatgaaaac
    17161 agcagaacct aattcagctg ttccaggtac tttattgaga tatactattc aagtagaaaa
    17221 cttaggacca gcaaatgctg aaaatgtaat attaaatgat gatattccat cttctattat
    17281 aaatccagaa tattcattgg atggaggagc aagttttcaa ccatggaatg gaagtcttaa
    17341 tataggaact ttaaactctg gaatttcatt aacagttttg attcaaggaa cagttagttt
    17401 aaattcttca gaatatatag taaatacagc aacagtttct tcacctacac ctgacccaga
    17461 tttaagcaac aatatatcta ccattattac accagttaac ccacaagcag gtatttctat
    17521 tataaaagta gctgacgagg atgtagctgt tccaggagaa gaatttgtat atacaattga
    17581 aatttttaat gaaggaccat ctaatgctac gaatgtagtt ttaacagatg atataccaga
    17641 tgttatttta aatccagaat attcattgga tggaggagca agtttccaac catggaatgg
    17701 aagtcttaat ataggtacag ttgtaccagg gcaattaata agaattatca taaagggatt
    17761 agtaagttca actgctacag gagatattac taatacagca gaagtaagtg cagatgtacc
    17821 agaaccagtt atggattcat caacagtgac aactcctata gttgcatcag cagatataga
    17881 agtaataaaa actagcaata tggatacagc agttccggga gaaactttta gttatactat
    17941 aacagttaca aatttaggac catctgctgc acaaagtatc gttttggcag atgatatacc
    18001 aaatgttatt ttaaatccag agtattcatt ggatggagga gtaactttcc aaccatggaa
    18061 tggaaatctt aatataggca ctttggatac tggagaaata agaagcatta taataagagg
    18121 aacagtgagc cagacagcag taggtacaat tataaataca gcaacaataa gttcacctac
    18181 acctgaccca aatccagata ataatacttc aacagatgaa acagatataa gtccattagc
    18241 agatatttct gtgataaaag gcaacgaacc acttgctata ccagggggaa gatttattta
    18301 tggaattgaa attgcaaatg caggaccatc ttttgcagag aacgtaactt taacagataa
    18361 tataccagct tctattttaa atccagagta ctcaatagat aatggagtga ctttccaacc
    18421 atggaatgga agccttaata taggtacttt agatgctgga gagataaaaa atatcataat
    18481 aaggggaaca gtaagtcaaa cagcaatagg tataattata aatacagcaa caataagttc
    18541 aactacacct gacccaaatc taaataataa tacttcaaca tcagaagcag aggtaagttc
    18601 atcagcagat atttctgtag taaaacgaag caatcaggct gttgttgtac caggagatgt
    18661 actagactat acaattgagg cgagaaatgc aggaccatct actgctcaga acgtaacttt
    18721 aacagataat ataccagttt ctattttaag tccagaatat tcaatagata atggagtgac
    18781 ttttcaacca tggactggaa gtcttaatat aggtacttta gatactggag ggataagaaa
    18841 tattataata aggggaatag taagtgaaac agcagtagga acaattataa atacagcaac
    18901 agtaagttca actacacctg acccaaatcc aaataataat acttcaacat cagaaataga
    18961 tataagttca tcagcagata tttctgtaat aaaattagca aataaaacag aagcatgtgt
    19021 aggagatgaa atagattttg taatagtagt atcaaatgca gggccagaaa atgctcaaaa
    19081 tgttacttta atagacaatg tttcagataa gcttaagaaa acagtatttt cacttgatag
    19141 gggtgtaagt ttccaaccat ggactggaag tcttaatata ggtacactaa cagctggaac
    19201 tttaagagtt attatattaa gaggaattat aaaatcaaca tgtcttgata aactaacaaa
    19261 tattgcagaa gttacttcaa caacaccaga ttcagacctt aacaataata tatcaagagt
    19321 gcaagtagaa ataaaacaat gttgttgtaa cgattgctgt tgtaacaact gtagtaattg
    19381 ttggtgtaat tgttgttgca aggattgtta ctgtaattgt tgtaaaaatg acttttggtg
    19441 ttgattttat aaaataattt tgtatacctt aaattaaact gaaataaaaa ataaattcac
    19501 atatgagtaa aacattttat aaatattaat aaattttcta tctttgatat ggaccacatt
    19561 cgtaatacta gttattaaa
//
LOCUS       DAEPXF010000068        18950 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain HB27
            SAMN13376389-rid12479423.denovo.072, whole genome shotgun sequence.
ACCESSION   DAEPXF010000068 DAEPXF010000000
VERSION     DAEPXF010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13376389
            Sequence Read Archive: SRR10518432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 18950)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18950)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001069624.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001069624.1
            Genome Coverage       :: 315x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/15/2021 01:54:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,165
            CDSs (total)                      :: 4,076
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 89
            rRNAs                             :: 10, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 10 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 19 of 69
            Pseudo Genes (incomplete)         :: 44 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 10 of 69
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18950
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13376389-rid12479423.denovo.072"
                     /strain="HB27"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="China: Hebei"
                     /collection_date="2017"
     gene            161..895
                     /locus_tag="KRL67_002029"
     CDS             161..895
                     /locus_tag="KRL67_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003722224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBH1522907.1"
                     /translation="MNKILRVKNIKKYYKSRGNVTKAIDNISFEVERGEYIGIMGASG
                     SGKTTLLNCISTIDKTTEGNIYIGDKDITTMDEDELSKFRREELGFIFQDFNLLDTLT
                     AHDNIALSLSINGVNSKEIDNKIKKIANKLGVHEILNKYPYEISGGQKQRIACARAII
                     TNPSLILADEPTGALDSKSAKLFLENLEVLNHELKATILMVTHDAFTASYCKRILFIK
                     DGKIFNELVRGDNTRQEFFDRIIKVI"
     gene            918..3047
                     /locus_tag="KRL67_002030"
     CDS             918..3047
                     /locus_tag="KRL67_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBH1522908.1"
                     /translation="MFFRKNKSRRVRNKRKNNYVSKVSSVSSESFKKLALNNVKKSLK
                     NYLIYFITLVFGVIILYTFNSLDNDIAILSNNALLSSSISFVRGIVSIFSILVCIIFA
                     FLIIYANNYLMKQRKKEFGIYSILGMDRRDINKLMFREMMIIGIFSLIIGIVFGVFIS
                     KGLSIFALKMLGISSSGFSFSILTTIKTIVFFGIVLLLVNKFNKKTIKKYKLIDLLNA
                     NKKNEVSVAKGKKSNLILFFLSIVSIVLAYIIPLKMKISTFVLCICLILILVGIYLFF
                     MSVSDFIMLKIKKHRFIYYKNLNPFTVSQISSQIKTMSMSITMICMLLFSSLIVIPFG
                     ISFGTYLIDDLQEATPYDVTLSKLFMNGDNIVEYSTKKSDINSKIPSLKDELIANDFP
                     LISFSKSTSDLRIYNLKNISQYDFITNDKDKTIKLKDNKLHIVSISDYNSTRKQSGLK
                     EINLKDNEFAINCASENYRQFYEKYIKSQPQNYITLNGVKLKLNQTSLYTNSYNTNTV
                     PLDLGTIIVPDAVIKDLNPIMITLNIDYKERSSNLENKFMDSYYKFEEKIDNSEDYSI
                     LYKSVIDGEKRALNTSFSFVAIYIGIILLISAGAILALQQLIESTVNKERFKLLSKLG
                     VNKKDMKKAIFIQISILFAVPLILALINALFISKVLFVIMPFIAETGVITNMLLTLGL
                     IIIVYGIYFIASLLESLNIVNNSKEII"
     gene            3264..3389
                     /locus_tag="KRL67_002031"
                     /pseudo
     CDS             3264..3389
                     /locus_tag="KRL67_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112778.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
     gene            complement(3983..4849)
                     /locus_tag="KRL67_002032"
     CDS             complement(3983..4849)
                     /locus_tag="KRL67_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001255868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside 6-adenylyltransferase AadE"
                     /protein_id="HBH1522909.1"
                     /translation="MRSEKEVYDIVLNFAKTDKRIRMVTLEGSRTNTNIPPDDFQDFD
                     ITFFVTDMDSFTSDDKWLDIFGERLILQKPEDMELFPAVEKGFSYLMLFTDDVKIDLT
                     LLPLELIDEYFTWDKLVKLLLDKDNRIVKPPIPTDIDYHLQKPTQRMFDDCCNEFWNT
                     TTYVVKGLCRKEILFAIDHMNDIVRKELLRMISWLIGIKQGFHFSLGKNYKFMKQYVP
                     EELWERLMSTYNMDSYPHMWESFEQCMALFREVSSEVACQLDYQYPLYDEKISNYVIR
                     QKKKYGIEDDNK"
     gene            complement(5015..5548)
                     /locus_tag="KRL67_002033"
     CDS             complement(5015..5548)
                     /locus_tag="KRL67_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007061211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBH1522910.1"
                     /translation="MNTIKLRPIITVEEKNTFVSEIQEAFQISYVSEFGEFEKQVLPT
                     EDIEESFNAKGAEAYIAEIGGERIGGAVIVIDDKTGYNSLHLLYVKSSAQNAGNGFKI
                     WKAIEELHPETRIWETHTPYYDKRNIHFYVNRCGFKIVEFFNPKHKDPHQISESAGNI
                     PKENNYFFRFEKEMKSR"
     gene            complement(5669..6469)
                     /locus_tag="KRL67_002034"
     CDS             complement(5669..6469)
                     /locus_tag="KRL67_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002606602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBH1522911.1"
                     /translation="MELSIQERLKDLRVERGLTLEQLAEQTHLSKSALGSYEGDNLKD
                     ISHYALIELAKFYEVTVDYLLGRSQTRNHPNADLADLHLSDDMIELLKSGLVDNSLLC
                     ELATHPDFPRLMADLEIYVNGVAGKQVQSANAIVDAVSATIMKQHNPGLTDPQLRQLI
                     AAHIDDDSFCRYVIQQDINKIALDLREAHKDDFFSVPEDNPLEDFLQTAEETTREDSD
                     PEQAAMMFICKRLKLNYKKLFEEEKKWLKRIAQKSDLLKNPKPQRGRK"
     gene            complement(6686..8125)
                     /locus_tag="KRL67_002035"
                     /note="aph(2'')-Ia"
     CDS             complement(6686..8125)
                     /locus_tag="KRL67_002035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(2'')-Ia"
                     /protein_id="HBH1522912.1"
                     /translation="MNIVENEICIRTLIDDDFPLMLKWLTDERVLEFYGGRDKKYTLE
                     SLKKHYTEPWEDEVFRVIIEYNNVPIGYGQIYKMYDELYTDYHYPKTDEIVYGMDQFI
                     GEPNYWSKGIGTRYIKLIFEFLKKERNANAVILDPHKNNPRAIRAYQKSGFRIIEDLP
                     EHELHEGKKEDCYLMEYRYDDNATNVKAMKYLIEHYFDNFKVDSIEIIGSGYDSVAYL
                     VNNEYIFKTKFSTNKKKGYAKEKAIYNFLNTNLETNVKIPNIEYSYISDELSILGYKE
                     IKGTFLTPEIYSTMSEEEQNLLKRDIASFLRQMHGLDYTDISECTIDNKQNVLEEYIL
                     LRETIYNDLTDIEKDYIESFMERLNATTVFEGKKCLCHNDFSCNHLLLDGNNRLTGII
                     DFGDSGIIDEYCDFIYLLEDSEEEIGTNFGEDILRMYGNIDIEKAKEYQDIVEEYYPI
                     ETIVYGIKNIKQEFIENGRKEIYKRTYKD"
     gene            complement(8126..8848)
                     /locus_tag="KRL67_002036"
     CDS             complement(8126..8848)
                     /locus_tag="KRL67_002036"
                     /inference="COORDINATES: protein motif:HMM:NF012792.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000393259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBH1522913.1"
                     /translation="MNIIRLSKEHIEECADLFIDVFTKAPWNDTYNSKGQVISYFQNY
                     INNNYFVGYGLKDQNCMIAISVGMKKPWINGMEYYIDQFCVKTDLQGKGIGSYFLKLI
                     ENEIQTEKMEEVLIDDNMVFDIDNLKGFLNDTSSFGFIAKENNKIIGFAYCYTLLRPD
                     GKTMFYLHSIGMLPNYQDKGYGSKLLSFIKEYSKEIGCSEMFLITDKGNPRACHVYEK
                     LGGKNDYKDEIVYVYDYEKGDK"
     gene            9160..9573
                     /locus_tag="KRL67_002037"
     CDS             9160..9573
                     /locus_tag="KRL67_002037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002578725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich VLP domain-containing protein"
                     /protein_id="HBH1522914.1"
                     /translation="MRNNPYKDLPPLERRPDGSLYRMTPAQRKQAASLIRRECCCCED
                     GNCIVLDDGDTCTCPQTVSFSVCCKWFRWAVLPLDGTLEAEIFRDKDLKRCVVCGGVF
                     VPKSNRAKYCPGCAARVHRRQKTESERKRRSAVDS"
     gene            9685..10080
                     /locus_tag="KRL67_002038"
     CDS             9685..10080
                     /locus_tag="KRL67_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009296947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HBH1522915.1"
                     /translation="MTNTIYIHQPEKAFSFTRLPNFLFEAPTFKPLSNEAKVLYAFIL
                     RRTELSRKNGWADDCGRIFLYYPICEVVDLLHCGRQKAVNTLRELQYAGLVEIQKQGC
                     GKPNRIFPKSYEAVPNTDFKKSRSGTPED"
     gene            10265..11209
                     /locus_tag="KRL67_002039"
     CDS             10265..11209
                     /locus_tag="KRL67_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002569775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid recombination protein"
                     /protein_id="HBH1522916.1"
                     /translation="MAQHAILRFEKHKGNPARPLEAHHERQKEQYASNPDIDTSRSKY
                     NFHIVKPEGRYYHFIQSRIEQAGCRTRKDSTRFVDTLITASPEFFKGKSPKEIQAFFQ
                     RAADFLIGRVGRENIVSAVVHMDEKTPHLHLTFVPLTKDNRLCAKEIIGNRANLTKWQ
                     DDFHAYMVEKYPDLERGESASKTGRKHIPTRIFKQAVSLSRQARAIEAALDGINPLNA
                     GKKKEEALSMLKKWFPQMENFSGQLKKYKVTINDLLAENEKLEARAKASEKGKMKDTM
                     ERAKLKSELDNLQRLVDRIPPDILAELKRQQRHTVKER"
     gene            11282..11476
                     /locus_tag="KRL67_002040"
     CDS             11282..11476
                     /locus_tag="KRL67_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002578728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH1522917.1"
                     /translation="MAKSESDIFTPRTGQVIQAENGTQYFVCGNNRIKISEHFAAGGK
                     PLGDLIVDVVRHTAEKAAST"
     gene            11622..13199
                     /locus_tag="KRL67_002041"
     CDS             11622..13199
                     /locus_tag="KRL67_002041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015520053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HBH1522918.1"
                     /translation="MRLSRDDENYGDSVSIETQRTILQQYAKEQGLHVVGEYVDDGWS
                     GTNFERPDFQRMMDDMEAGKVNCIVTKDLSRFGREHVMMDYYLEFLFPEKRVRYIAVA
                     ENEDTEKGLSDFVPFKNLFNEWFAKDTSRKVKAAFKAKFATGQRIGAYAPIGYRKHPE
                     IKNKLIIDEETRWIVEKIFDLAIHGRGAASITRILIAEKVPTPGFINFQRDGTFANIY
                     AGAPEEKSYAWTIAQVKSIMKDETYIGHTIHYRETNISFKNKRRVRKPQSEWVRVENT
                     QEPIISEQVFRQVQEQIANRRRKCKDGTTQIFSGLVKCADCGWSLSYGENRQNSKPYG
                     HYHCSKYGQGTRQCSMHYIRYDVLYAYVLSRLQYWSGLVQHDEERLLKQLLNATDKGQ
                     AAARKKQAAELKKAEKRKAEVDTLFARMYEDWAAGRITEYNFSMLSGKYQSEQAELDE
                     KIEQLQSAIATESQNAVDAEKWIALMKECVNPTELTAELLNMLIEKIVVHEAVKGEDG
                     SREQEVEIFYRFIGKID"
     gene            13249..14328
                     /locus_tag="KRL67_002042"
     CDS             13249..14328
                     /locus_tag="KRL67_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HBH1522919.1"
                     /translation="MELLPLLADFFNVSIDKLLNYKIDLSEEEVMKIYKELESGFARI
                     EIDLSTEEPKEECRQDLESVKKLSNMYIEKYPKSYLLKLRICSLYQMYSYKFGKSELN
                     NRVKETTNILEDIVRNTDDIQIKETALIILSNAYCMLEDYEKAELYLNMIHKSIGDTS
                     VNLAMIYLKQNRLEEAEILLQNKLFSNVFNMSMDCKGIINVYKNQYKELKKKLENRNS
                     NKNAIERKMEYIKNKLLGYANLSLEIKKMLSEDKGAFFSMYMDYMELSLIFLFFNMKE
                     EAKKALYSLKEILEKYPIHESLDVSQMRFFDKVESKNLYTFNIYTNLLIMLNDNSYNE
                     LREEPIFKGVIEKILEMEKMLKNEE"
     gene            complement(14750..15271)
                     /locus_tag="KRL67_002043"
     CDS             complement(14750..15271)
                     /locus_tag="KRL67_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBH1522920.1"
                     /translation="MKFERLRTSENKLYYKAMELYKISFPFHEQRKSSLQAEIMKNKE
                     YQFNLIYDKNQFVGIILCWETDSFIYVEHFCIFPEMRNNRYGQRALELLNKKGKTVIL
                     EIDLPIDEISIYRKAFYERVKYKTNDYEHIHPPYHEKFNGHNLIVMSYPEKLSKMEYD
                     NFNQYLRNNVMSF"
     gene            15627..16424
                     /locus_tag="KRL67_002044"
     CDS             15627..16424
                     /locus_tag="KRL67_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="G5 domain-containing protein"
                     /protein_id="HBH1522921.1"
                     /translation="MEKREKRIIISSLLSVSILMGLVSIYSILNKEDISLTVKGQEQK
                     VSSFKKTVEELLDEQGVKYNSEDKINPSLDTELKDDMKIKVVKVTKSKKEEIEKIPFD
                     TKHVNDSNLLKGKSKVYQEGQEGEKKLVYNLTYHDGKLVKKVLSKEVISKEPTTKIIK
                     YGTKEKVLIASRGANIRGGKHMKVVATAYAGDTITSTGTTPRWGVIAVDPRVIPYGTK
                     VYIPKLGMTFVAEDCGGAIKGNRIDIFMNSEGKASNWGRKSIDIYLH"
     gene            16534..17124
                     /locus_tag="KRL67_002045"
     CDS             16534..17124
                     /locus_tag="KRL67_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HBH1522922.1"
                     /translation="MKIVCLGDSLTYGFGVSRSNSWTNIVNKETRLEIVNKGINGDTT
                     SGMLARFNEDVVKNSPDIVFIMGGTNDFIAGARNEIINSNIMAMVHQSYSKNIIPIIG
                     IPLKPDIPNVRADWSCFTDFNIVSRNLESYSYWIKKFCMTFNTNFVDFYSEYNKNMEL
                     EGYKKLYFDGLHPTKEGHRIMADIFINSINKYIEEL"
     gene            17190..18887
                     /locus_tag="KRL67_002046"
     CDS             17190..18887
                     /locus_tag="KRL67_002046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HBH1522923.1"
                     /translation="MDYTKTYEEWTKGSYFDEDTKLELEKIKNNEKEIEDRFYKDLEF
                     GTAGLRGIIEAGTNRINKYTVRRATFGLANYILENTTKEETSRGVVIAHDNRHKSRQF
                     CIESANTLAACGIKAYIFDSLRTTPELSFAVRSLNAIAGIVITASHNPPEYNGYKVYW
                     EDGAQVMPEIANAITEKVNSIHDYSTIPTLTGANKNLVVLLDESQDTKFIEAVKSQII
                     RKDLVKNVGKSFKIVYTPLCGTGNIPIRRALKEVGFENIIVVPEEENPDPNFAGLDYP
                     NPEEKKALNRGILLAKEKGADLVIATDPDCDRVGVAVKTTTGEYALLTGNQIGGMLTH
                     YIIEGLKENNKLKENPTIIKTIVTSEFGADIAKSNNVDVLNVLTGFKFIGEKIKLFEQ
                     NKNRSYVFGYEESYGYLVGTHARDKDGVVSSLLISEMAAFYYSKGMSLYDGLIELYKK
                     YGFFKEQTISLTLKGIEGVEKIKEIISYFRENQIDYINSIKVVDKKDYKNGIDNLPKS
                     DVLKYFLEDESWVAIRPSGTEPKLKFYIAVKGASDIEADKKIQGLKKYIDDMVEKLK"
BASE COUNT         6522 a         3094 c         3494 g         5840 t
ORIGIN      
        1 aaattaaatt attctagaaa aggaactaga aataagtagg tgctattgat tttgataaat
       61 ataagaagct tatttatgaa attgataaga aaaaaatacg taaaaaatat aaatttattt
      121 gtttttaatt tcaaaataaa acttaatgga ggtaatggat atgaacaaaa tactgagagt
      181 aaaaaacata aaaaaatact ataaaagtag aggtaacgtt acaaaagcta ttgataatat
      241 tagttttgaa gttgaaaggg gagagtatat aggtattatg ggagcttcag gaagtggtaa
      301 aactacttta ttgaattgta tttctactat agataaaacg acagaaggta atatatacat
      361 aggagataaa gacataacaa ctatggatga agatgagcta tctaaatttc gtagagaaga
      421 actaggtttt atatttcaag attttaattt attagacaca cttacagcac atgataatat
      481 agcattgtct ttatctatta atggagttaa ttcaaaagaa atagataata aaataaaaaa
      541 aatagcaaat aaattaggtg ttcatgaaat acttaataag tatccatatg aaatatctgg
      601 tggtcaaaag caaagaatag catgtgcaag agctataata acaaatccat cactaatatt
      661 agcagatgaa cctacaggag ctctggactc taagtcagca aaattatttt tggaaaacct
      721 tgaagtttta aatcatgaat taaaagctac aatattaatg gttacacatg atgcttttac
      781 agctagttat tgcaaaagga tattatttat aaaagatggt aaaatattca atgagttggt
      841 tagaggagat aatactcgtc aagaattttt tgatagaata ataaaagtaa tataagctct
      901 tgggggtgat gataaacatg ttttttagaa aaaataaatc aagaagagta agaaataaaa
      961 gaaaaaataa ttatgtttca aaagtgtcaa gtgtgtcaag tgaatcattt aaaaaattgg
     1021 ccttaaataa tgtgaaaaaa agtctgaaaa attatttgat ttattttata acccttgtat
     1081 ttggagttat tattttatac acttttaatt ctttagacaa tgatatagca attcttagta
     1141 ataatgcttt attgagttca agtatatcat ttgttagagg tattgtaagt attttttcaa
     1201 ttctagtgtg tattatattt gcatttttaa ttatttatgc aaataactat ttaatgaaac
     1261 aacgtaaaaa agagtttgga atatacagta ttcttggaat ggacagaaga gacataaata
     1321 aattaatgtt tagagaaatg atgataatag gtatattttc tttaataata ggtattgtat
     1381 ttggagtatt tatctctaag ggacttagta tatttgcctt aaagatgtta ggtatatcat
     1441 catcaggatt ttctttctca atacttacaa ctattaaaac aatagtattt tttggcattg
     1501 tattgttatt agtcaataaa tttaataaaa aaacaataaa aaaatacaag ttaatagact
     1561 tattaaatgc aaataaaaag aatgaagttt cagttgcaaa agggaaaaaa tctaatttga
     1621 tattattctt tttatccata gtgtcaattg ttttagcata tataatacca ttgaaaatga
     1681 aaatatctac gtttgtacta tgcatatgtt taattttaat attagttgga atttatttgt
     1741 tttttatgtc agtatctgat tttataatgt taaaaataaa gaagcataga tttatatact
     1801 ataaaaattt aaatccattt acagtaagtc aaataagtag tcaaatcaaa acaatgagta
     1861 tgtctatcac tatgatttgt atgctattat tttcttcttt gatagtaata ccttttggta
     1921 tatcctttgg aacatattta atagatgatt tacaagaagc gacaccttat gatgtaacat
     1981 tatctaaatt atttatgaat ggagataaca ttgtagaata cagtacaaag aaaagtgata
     2041 taaatagtaa aattccttca cttaaagatg aactaatagc aaatgatttt cccctaattt
     2101 cattttctaa aagtacaagt gatttaagga tatataattt aaagaacata agtcagtacg
     2161 attttataac aaatgataag gataagacaa ttaaactgaa agataacaaa cttcatattg
     2221 tctcaattag tgattataat tctactagaa agcaaagtgg tttaaaagaa ataaatctaa
     2281 aggataatga atttgcaata aattgtgcct cagaaaatta tagacaattt tatgaaaagt
     2341 acattaagag tcagccacag aattatataa cattaaatgg agtaaagctt aaattaaatc
     2401 agacaagctt atataccaat tcttacaata ctaatacagt tcctttagat ttgggaacca
     2461 taattgttcc agatgctgta ataaaggatt taaatcctat tatgattaca ttaaatattg
     2521 attacaaaga aagaagtagt aatcttgaaa ataaatttat ggattcatac tataaatttg
     2581 aagagaaaat cgataattct gaggattata gtattttata taaatcagtt atagatggag
     2641 aaaaaagagc tttgaacact tccttttctt ttgtagcaat atatataggt attatattat
     2701 taatatcagc aggtgcaata ctagcattgc aacaacttat agaatcaact gttaataaag
     2761 aaagatttaa attattaagt aaacttggag taaataagaa agatatgaaa aaagctattt
     2821 ttatacaaat atcaatatta tttgcagtac ctttaatatt agctcttata aatgctttgt
     2881 ttatatcaaa agtcttattt gttataatgc catttattgc agagacaggt gttattacaa
     2941 atatgttact aacgttaggt cttattatca ttgtatatgg aatttatttt attgcaagtt
     3001 tacttgaaag cttaaatata gtaaataata gtaaagagat aatttaagtt tttaaaagac
     3061 tatttagtgt taatctaatt aattaagcta cacttaagtt agcttaatta atagattcta
     3121 tatttcaagc caatgagata cggttttaga ttaaaatata accaatggtt aaattacaga
     3181 cattttgtta actttccctc gatttactag taaatatttt ttttatatac ttaaattaca
     3241 ccaaatgtac tagggaggta tatatgcaaa ttaaaatagg taaagtcata cagcatctta
     3301 gaaaagaacg aaatttaact caagaacaat tagcaaagtt tataggtgtg tcaacaccag
     3361 cagtatctaa gtgggagagt ggggattcac ttagataaag ataccacacc attcccacgc
     3421 tgacttttgc tcaaccgata cagccggagg gctggataac aagaaaaggc ggtatgcgcc
     3481 ccgtagaggg gtagcgtacc gccttttctt gtgggggttt ccaaaggggg caacgccccc
     3541 atttggcaca cgactttgcg aagcaaagtg tagtgtgtta tacgctctgt cggcgttgcc
     3601 gtgaaaatgc cgttgccgcc ggggaggggc ggcaggaaaa caggactgtg cttgtgtggc
     3661 gtggagccgc aagcgcaggt ctgttttcat cagcagacag ggcgtatatg aaagcccccg
     3721 tccctcgtcc tacgctccga cttgtggaca aagtgtcccg aagttgtggc tacactcccg
     3781 gaaaagtaac aggacagcgg gtaaccgtca aggctgaaat gaacgggttt acacccggcc
     3841 ttgaccgcca cccgccgtcc cactgaatgg gtggacaagg cggccaatcc ctcaaagtgc
     3901 ttgtccgcgc tctttctgat tttgaggtat tcagtttttc aaaattgaat aatcaaaata
     3961 aattttttcg attgaaaaaa ttttatttgt tatcatcttc aatgccatat tttttctttt
     4021 gccgaatcac ataattactg attttttcat catatagtgg atactggtaa tccaactggc
     4081 atgccacttc tgacgaaacc tcccggaaca atgccataca ttgttcaaag gattcccaca
     4141 tatggggata ggaatccata ttataagtgg acataagtcg ttcccacaat tcctctggga
     4201 catattgctt cataaattta tagttttttc ccaaactgaa atgaaatccc tgtttgatac
     4261 caatcagcca ggaaatcatg cgaagcaatt cttttcgtac tatatcattc atatggtcaa
     4321 tagcaaaaag aatttctttg cggcataagc cctttactac atatgttgta gtattccaaa
     4381 attcattaca gcaatcgtca aacattcttt gagtaggctt ctgcaagtgg tagtctatat
     4441 ccgttggtat tggcggcttt acgatacggt tgtctttatc caacagtaac tttaccagtt
     4501 tatcccatgt aaaatactcg tctatcagtt ccagcggcag caaagttaaa tctatcttaa
     4561 catcatcagt aaacagcatt aaatatgaaa atcccttttc tacagctgga aataattcca
     4621 tatcttccgg cttttgcaga atcaaccttt caccaaatat atctagccat ttatcatcac
     4681 ttgtgaagct gtccatatcc gtaacaaaaa aagtaatatc aaaatcctga aaatcatcag
     4741 gcggaatatt tgtatttgtt ctagatcctt ccaaagtaac catgcgaatg cgtttgtctg
     4801 tttttgcaaa attcaaaaca atatcataaa cttccttttc tgatctcatt tttatctcct
     4861 ccaattattg aaataggtgt gaagccattt tagggctttc ccgccttgat tgctcatcag
     4921 caaaggcggg cggggctgtc aacggcggcg cgtatgcgcc gttcatcttg accgttgact
     4981 ggctcggctg gctttgctat cctccgataa attactatct ggatttcatt tctttttcaa
     5041 atctaaagaa ataattgttc tcttttggta tgttgcctgc gctttcactt atttggtgtg
     5101 ggtctttgtg cttaggatta aaaaattcta caatcttgaa accacaacga tttacataaa
     5161 aatgaatgtt ccgcttatcg taatatgggg tatgcgtctc ccaaattctt gtttcaggat
     5221 gtagttcttc aatcgctttc catatcttaa atccatttcc agcattttgt gcgcttgatt
     5281 tcacatataa aagatgaagt gagttgtagc cggtcttatc atctattaca atgactgctc
     5341 cgccgattct ttctccgcca atttctgcga tatatgcttc agcgcctttt gcgttaaaag
     5401 attcttctat atcttccgtg ggaagaactt gcttttcaaa ttctccaaac tctgacacat
     5461 aagaaatttg aaaagcctct tgtatttcac ttacaaatgt gtttttttct tcaacggtaa
     5521 tgattggtct taattttatt gtgttcatac ttccttgctc tccttaaatt ttatagtctg
     5581 gttcatcaaa gaagtatgtc tttattctct cccaccccaa tcaaatagtt tcatagttca
     5641 ccactccaaa tctcgatttt ccgttctcct actttcttcc ccgctggggc tttggatttt
     5701 tcagcaagtc agacttttgt gcaattcttt ttagccattt cttttcttcc tcgaacagct
     5761 tcttgtaatt cagtttgagc cgcttgcaga taaacatcat agccgcctgc tccgggtcgc
     5821 tgtcctctct ggtggtttcc tctgcggtct gcaagaaatc ttccagtggg ttgtcctctg
     5881 ggacactgaa aaaatcgtcc ttatgggctt cccgcaggtc gagggctatc ttgtttatgt
     5941 cctgctgtat cacatagcgg caaaagctgt cgtcgtcaat atgggcggcg ataagctgtc
     6001 gcagctgcgg gtcagtcaag ccgggattgt gttgtttcat aattgttgca ctcacagcgt
     6061 ccacaatggc gtttgcgctc tgcacctgtt ttcctgccac gccgttcaca tagatttcaa
     6121 ggtcggccat gagccgggga aaatccgggt gggtcgccag ttcacacaaa agagaattgt
     6181 ccaccagccc gctttttaat agttcaatca tgtcatcact caaatgcagg tctgcaagat
     6241 cggcgtttgg gtgatttctt gtttgagaac ggcccagcag ataatcaacg gtcacttcgt
     6301 aaaactttgc caactcaata agggcataat ggctgatgtc cttgagattg tccccctcat
     6361 aactgcctaa agcagacttg gagaggtggg tctgctccgc aagctgttcc agcgtcagcc
     6421 cacgctctac acgcaggtct ttcaatcgtt cttgtatgga tagttccatg ctctcctcct
     6481 catccgctta agcggccgat atttattttg tgagagtaat aagttcatca actgattgga
     6541 agccgttttt cttataaaat ttttcggccg gaaaacctcg ttcagtattc aaaatgattg
     6601 cgttcatttt tcctttatta tttttcattt ttttaaacta tttaatacta atgtctttta
     6661 taatagcttt tcatatatta tataatcaat ctttataagt ccttttataa atttcttttc
     6721 taccattttc gataaattcc tgtttaatat ttttaattcc ataaacaata gtttcaatag
     6781 gataatattc ttcaactata tcttgatatt cttttgcttt ctcaatatct atatttccat
     6841 acattcttaa tatatcttct ccaaaatttg ttcctatttc ttcttcacta tcttcaagta
     6901 agtatataaa atcacaatat tcatctataa ttccagaatc tccaaaatca attattccag
     6961 ttaatctatt attgccatct aacaatagat gattacaact aaaatcatta tggcataaac
     7021 actttttacc ctcaaaaact gttgttgcat ttagtctttc cataaaactt tctatataat
     7081 ctttttctat atcagttaaa tcattataaa tagtttcacg caacaatata tactcttcta
     7141 atacattttg tttattatca atagtacatt cactaatatc tgtataatct aaaccgtgca
     7201 tttgtcttaa aaaactggca atatctcgtt ttaacaaatt ttgttcttct tctgacatag
     7261 tagaataaat ttctggtgtt aaaaaagttc ctttaatttc tttataacct agtatagata
     7321 attcatcact aatatacgaa tattcaatat taggaatttt tacattagtt tctaaatttg
     7381 tatttaaaaa attatatatt gctttttctt ttgcataacc ttttttctta ttagtactaa
     7441 attttgtttt aaaaatgtat tcattattaa ctaaatatgc cacactatca taaccactac
     7501 cgattatttc aatactatct actttgaaat tatcaaagta atgctcaatt aaatatttca
     7561 ttgccttaac atttgtggca ttatcatcat atctatattc cattaaataa caatcttctt
     7621 ttttgccctc gtgtaattca tgttctggca aatcttcaat aattctaaaa ccagattttt
     7681 ggtatgccct tattgctctt ggattatttt tatgagggtc taaaataact gcattagcat
     7741 ttctttcttt tttcaaaaat tcaaaaatca atttaatata tcttgtacca attcctttac
     7801 tccaataatt tggctctcct ataaattgat ccataccata gactatctca tcagtttttg
     7861 gataatgata atcagtatat aactcatcat acattttata tatttgtcca tatccaatag
     7921 gaacattgtt atattcaata attactctaa aaacttcatc ttcccaaggc tctgtataat
     7981 gtttttttaa tgattctaat gtatattttt tatctctacc accataaaat tctaatactc
     8041 tttcatcagt taaccatttt aacatcaaag gaaaatcatc atctattaaa gttcttatac
     8101 atatttcatt ttcaactata ttcatttatt tatcaccttt ttcataatca tatacatata
     8161 ctatttcatc tttataatca tttttaccac ctaatttttc atatacatgg caagctctag
     8221 gattaccttt atcagttatt aaaaacattt cagaacaacc aatctcttta gaatattcct
     8281 taataaaaga taataatttt gaaccataac ctttgtcttg atagttaggt aacattccta
     8341 ttgagtgtaa ataaaacatt gtttttccat caggtcttaa aagtgtatag caatatgcaa
     8401 atcctataat tttattattt tctttagcta taaacccaaa tgaactggta tcattaagaa
     8461 atccttttaa attatcaata tcaaaaacca tattatcatc aattaaaact tcttccattt
     8521 tttctgtctg tatctcattt tcaatcaatt tcaaaaaata acttccaatc cctttccctt
     8581 gcaagtcagt ctttacacaa aactggtcga tataatattc cattccgtta atccatggtt
     8641 tcttcattcc cacgctgata gcaatcatac agttttggtc ttttaagcca taccctacaa
     8701 agtagttatt gtttatgtag ttttgaaaat aacttatcac ctgccctttg gaattgtatg
     8761 tatcattcca gggggctttt gtgaacacat caataaataa atctgcacat tcctcaatgt
     8821 gctcctttga cagtctaatt atgttcaaaa attctcctcc ctcatatttt cttccattat
     8881 accacaattc cgagagcgtg gaaataggga gttttccggg cggatttcct acctcttgga
     8941 tatacgggac gggcggttat ttaggtgtag acttaggata gttcatcgat ggacagcacc
     9001 ttgaaaacag aatgaccgtc cgaaaaggaa taccccggct ggggaagcac cgcaaggaac
     9061 cggacttcgg gacaaaatgt cccgaagctg cggggagcgt gccaaagctg gaaatccttt
     9121 cggggaaaac ggcaacggaa agcaaaatgg agggaacgca tgagaaataa cccctataaa
     9181 gacttgccgc cgctggaacg caggccggac ggttcccttt accgcatgac accggcacag
     9241 aggaaacagg cggccagcct gatacgccgg gagtgctgtt gctgtgagga cggcaactgc
     9301 attgtccttg acgatgggga cacctgcacc tgcccgcaga cggtttcttt ctcggtctgc
     9361 tgtaagtggt tccgctgggc ggtcttgccg ctggacggga cgctggaagc ggagattttc
     9421 cgggataagg acttgaaacg ctgtgtggtc tgcggcggtg tgttcgtccc caaatccaac
     9481 cgggcaaaat actgccccgg ctgtgccgcc agagttcaca ggcggcagaa aacagaaagt
     9541 gaacggaaaa ggaggtctgc tgtggacagt taggagcgaa aaaagccttg atttatcagg
     9601 ctttgcaagc cccaaaccgg ggcaggtggt ataaagtatc gcccgccccg gaaaacgggc
     9661 ttctaaccgt ccacaaaacg cactatgaca aataccatct atatccatca gccggaaaag
     9721 gcgttcagct tcacccggct cccgaatttc ctctttgaag cccccacatt caagcccctg
     9781 tccaacgagg caaaggttct gtacgccttt atcctgcgcc ggacagagtt gtcccgcaag
     9841 aatgggtggg cggatgactg cggacggatt ttcctgtatt accccatctg cgaggtggtt
     9901 gacctgctcc actgtgggcg gcagaaagcg gtgaacaccc tgcgggaact gcaatacgcc
     9961 ggactggtgg aaatccagaa gcagggctgt ggaaaaccca accgcatttt cccaaaatcc
    10021 tatgaagcgg ttccaaacac tgacttcaag aaatcccgtt ctggtacgcc ggaggactga
    10081 aaaccgtacc catgaagtac ggaaaccgcc cctcttgaag tacgaaaacc ggacggtata
    10141 tagaaataca aagattaaaa tgatttattt atatccattc cattcctatc gtatcagaga
    10201 taattccggc gggattttcc ctgtggaaaa ccccggatag gaaaggaatg gggaaaggag
    10261 cagaatggca caacacgcaa ttttgcggtt tgagaagcac aagggcaacc cggcaaggcc
    10321 gctggaagcc catcacgaaa gacagaaaga acaatacgcc agcaaccccg acattgacac
    10381 aagccggagt aaatacaact tccatatcgt caagccggag ggacgctatt accacttcat
    10441 tcagagccgt atcgagcagg ctggatgccg gacgaggaaa gacagcacac ggtttgtcga
    10501 tacgctgata accgccagcc cggagttctt caaggggaaa tccccaaaag agatacaggc
    10561 gttcttccaa agggcggcag atttcctcat tggccgggta ggacgggaaa atatcgtgtc
    10621 ggcggtggta cacatggacg agaaaacgcc ccacctgcat ttgacctttg ttccgctgac
    10681 aaaggacaac cgcctgtgcg ctaaggagat tatcggcaac cgggcaaacc tgacgaagtg
    10741 gcaggacgat tttcacgcct atatggtgga gaaatatccc gacttggagc gtggggaaag
    10801 tgccagcaag acaggccgga agcatatccc cacccggatt ttcaaacagg cggtttccct
    10861 ctcccggcag gcaagagcca ttgaagccgc ccttgacggc attaacccgc tgaacgccgg
    10921 aaagaaaaaa gaggaagccc tctccatgct gaaaaagtgg ttcccgcaga tggagaactt
    10981 ctccgggcaa ctgaaaaagt acaaggtcac aatcaatgac ctgttggcgg agaatgagaa
    11041 gctggaagca agggcaaagg ccagcgaaaa aggaaagatg aaagatacga tggaacgggc
    11101 aaagctgaaa agcgaactgg acaatttaca gcggctggtt gaccgtatcc cgccggatat
    11161 actggcggaa ctgaaacggc agcagcggca cacggtaaag gaaaggtgat agatataagc
    11221 agaaattttc gccgcctttg tgcggcattg taccttgaaa actgaatatg gaggacactg
    11281 gatggcaaaa agcgaaagcg atattttcac accccggaca gggcaggtca tacaagcaga
    11341 gaacggcacg cagtattttg tatgtgggaa caaccgtata aaaatctccg aacacttcgc
    11401 cgcaggcggg aagcccctcg gtgatctgat tgtagatgtg gtgcggcata ccgcagaaaa
    11461 agccgcttca acctgatagc ccatcattga taacacgccc acgcttatga tataattgcc
    11521 atagagcaaa agtattgtaa gcgtgggttg tttctttaga aggaggattt ttaacggtga
    11581 aacaacctta caatactacg atttacaaca cggcgcttta tatgagattg agccgggacg
    11641 atgaaaacta tggtgacagc gtaagcattg aaacacagcg gacaatcctg caacagtacg
    11701 caaaggaaca ggggcttcat gtagtcggtg agtatgtgga cgatggctgg tcggggacga
    11761 actttgaacg gccggatttt cagcgcatga tggacgatat ggaagccgga aaagtaaact
    11821 gcattgtcac gaaagacctg tcccgtttcg ggcgggaaca tgtgatgatg gactactatc
    11881 tggaatttct gttcccggag aaacgggttc gctacatcgc cgttgcggag aatgaggaca
    11941 cagagaaagg gctttccgat tttgtcccgt tcaaaaacct gttcaatgaa tggtttgcga
    12001 aagatacaag ccgcaaggta aaggccgctt tcaaagcaaa gtttgccaca ggacagcgta
    12061 tcggcgccta tgctcccatc gggtacagga aacacccgga aatcaaaaac aagctgataa
    12121 tcgacgagga aacccgctgg atagtggaga agatttttga ccttgctatt catggcagag
    12181 gggcggccag tatcacaaga atactgattg cggaaaaggt tcccacaccg ggatttatca
    12241 acttccagcg tgacgggact tttgcaaaca tctatgcggg tgcgccggag gaaaaaagct
    12301 acgcatggac gatagcccag gtcaagagca ttatgaaaga cgaaacctat atcggccata
    12361 ccattcatta ccgggaaacg aacatttcct ttaagaacaa gcggagggta cgcaagcccc
    12421 aaagtgaatg ggtgcgggtg gagaacacgc aggagccgat tatcagcgag caggttttcc
    12481 ggcaggtaca ggagcagata gcaaaccgcc gcaggaagtg caaggatggt acaacgcaga
    12541 ttttttccgg attggtaaaa tgtgcggatt gcggctggtc gctgtcctat ggggagaaca
    12601 ggcagaacag caagccttac ggccattatc attgtagcaa gtacgggcag ggcacacgcc
    12661 aatgctccat gcactatatc cgttatgatg tgctttatgc ctatgtcctc tcccgtctgc
    12721 aatactggtc ggggctggta cagcatgatg aagaacggct cttgaagcag ctgttaaatg
    12781 ccactgataa gggacaggct gccgcaagaa agaagcaggc cgcagaactg aaaaaggcag
    12841 agaagcgaaa agccgaagtc gatactttgt ttgcccggat gtatgaggac tgggcggcgg
    12901 ggcgtatcac ggaatacaac ttctctatgc tgtccgggaa gtaccaaagc gaacaggctg
    12961 aactggacga aaagattgaa cagcttcaat ccgctatcgc cactgaaagc cagaacgccg
    13021 tagacgcaga aaaatggatt gccttgatga aagagtgcgt caatccaaca gaactgaccg
    13081 ccgaactttt gaatatgctg attgaaaaaa tcgttgtcca tgaagcggtc aaaggtgagg
    13141 acggaagccg ggaacaggag gtagaaatct tctaccgctt tatcggcaaa attgattgaa
    13201 tgataccaat atctttaact atgtgatacc ggaaattctt acccagatat agaattatta
    13261 cctttattag cagacttttt taatgtgtct attgataagc ttttaaatta caagattgat
    13321 ttaagtgaag aagaagtaat gaaaatttat aaagaattag aatcaggttt tgctagaatt
    13381 gaaattgatt tatcaacaga agaaccaaaa gaagagtgta gacaagactt agaatctgta
    13441 aaaaaacttt ctaatatgta tatagagaag tatcctaaaa gctatttgtt gaagctaagg
    13501 atatgttcgc tgtatcagat gtattcatat aaatttggta agagtgaact taataacaga
    13561 gtaaaagaaa caacgaatat tcttgaagat atagtaagaa atacagatga tatccaaata
    13621 aaagaaacag ctcttattat attatcaaac gcatattgta tgttggagga ttatgaaaag
    13681 gcagagttgt acttaaatat gatacataag tcaatagggg atacaagtgt taatttagct
    13741 atgatatatt taaagcaaaa tagacttgaa gaagcagaaa tattgcttca aaataaattg
    13801 tttagtaatg tattcaatat gagcatggat tgtaagggaa taatcaatgt atacaaaaat
    13861 caatacaagg aattaaagaa aaaattggaa aatagaaatt ctaataaaaa tgcaattgag
    13921 agaaaaatgg agtatataaa aaataagttg ctggggtatg caaatctttc tttagaaata
    13981 aaaaaaatgc tcagtgaaga taaaggtgca ttttttagta tgtatatgga ttatatggaa
    14041 ttgtctctaa tttttttatt ttttaacatg aaagaagaag caaaaaaagc cttatatagt
    14101 ctaaaagaaa tattagaaaa atatcctata catgaaagtt tggatgtaag tcaaatgaga
    14161 ttttttgata aggtagagtc taaaaattta tatactttta atatatatac aaacttgtta
    14221 attatgctta atgataatag ttataatgag cttagagaag aaccaatttt taagggtgta
    14281 attgaaaaga ttttagagat ggaaaaaatg ttaaaaaatg aagaataagt aaaaatttaa
    14341 tataggactt aagactaatc aaattaaaag gaagtgtttc aaaataaaaa tttaaatcac
    14401 cttttttgtt ggtcttaaac aattatttag aaaaatagag gtttcaaaat aaaaatctga
    14461 attatctgtc ttattacaca aaaatagaaa cctaaatttg caaaaataaa ataaagtttg
    14521 ttatataaaa tgtaattttg tctataaaat ctcacatgtt ttcttaactt taagtgtgaa
    14581 aaagagggtg tctcaaaatg aactttttag ttcatgaggc atccttcttt gtatgaaatc
    14641 gaatatatct ttataattta aacaatgaaa aagaatctta tttctatttt gagacatctc
    14701 ttttatttaa ttatgaaatt ttacacagtt caataaatag gaattacatt tagaaactca
    14761 tgacattatt tcttaaatac tgattaaaat tatcatattc cattttagac aatttttctg
    14821 ggtaagacat tacaatcaga ttatgtccat taaatttctc atggtatggt ggatgaatgt
    14881 gttcataatc atttgtctta tattttactc tttcatagaa cgccttgcga taaattgaaa
    14941 tctcatcaat tggaaggtca atttctaaaa taacagtttt tcctttctta tttaaaagct
    15001 ctaatgctct ttgtccatat ctattattac gcatttctgg aaaaatgcag aaatgttcta
    15061 catagataaa gctatctgtt tcccaacata aaattatgcc gacaaactgg tttttatcat
    15121 aaattaaatt gaattgatat tctttatttt tcataatttc tgcttgcaaa gaagattttc
    15181 tttgttcatg aaatggaaaa ctaattttat ataattccat agccttgtaa tataatttat
    15241 tttcactggt tcttaaccgt tcaaatttca ctttactctc cctaatcacg aaagttctaa
    15301 tttatttatg agttcatttt accatagtca ttatttgcat acaatgtgtt ttaagttgag
    15361 tgtcatttct ttcttttatt tatgtttata tctcatagac taatgtataa ttgcttaaga
    15421 cataacttaa aactattata ttaagctttg ataatctaaa aacagtatat ttatggactt
    15481 ttctaggtct aaaattaatc ttaataattt tagaagttac aaaaaagtta cataaatttg
    15541 gaaagctata atatacatat ataatataag gttaaaactg taatgaataa tattaattta
    15601 aaatttgaac atggaaggag attattatgg agaaaagaga aaagaggata ataatatcat
    15661 ctttattaag tgtatcaatt ttaatgggtt tggtaagtat atattctata ttaaataaag
    15721 aagacataag tttaacagtt aaaggtcaag agcaaaaagt atcttccttt aagaaaacag
    15781 ttgaggaact tttggatgaa cagggtgtaa agtataattc tgaagataag attaatccaa
    15841 gtttagatac agaactaaaa gatgatatga aaataaaagt tgttaaggta actaaaagta
    15901 aaaaagaaga gattgaaaaa attccatttg atacaaagca tgtaaatgat agtaatttgt
    15961 taaaaggaaa atctaaagtt tatcaagaag gtcaagaggg agaaaaaaaa ctagtctata
    16021 atttaactta ccatgatgga aagttagtca aaaaagtctt atcaaaagaa gtaatatcta
    16081 aggagccaac tacaaaaatt ataaaatatg gaactaaaga aaaagtatta atagcatcaa
    16141 gaggagcaaa tataagagga ggcaaacata tgaaagtggt tgccactgca tatgcaggag
    16201 atacaataac atctactggt acaactccaa gatggggtgt tattgcagtt gacccacgtg
    16261 taataccata tggaacaaaa gtatatatac ctaagttggg tatgactttt gtagcagaag
    16321 attgtggagg tgcaattaaa ggtaatagaa tagatatttt tatgaacagt gaaggaaaag
    16381 cctcaaattg gggaaggaaa agtatagata tatatctaca ttaaattagt gaatagaata
    16441 actttacttg aaggacaaaa aagtataaaa tatgaattaa ctaaactgtt agattattct
    16501 taaaacataa aaatatatct aaggggggat aagatgaaaa tagtctgctt aggagacagt
    16561 ttaacatatg gatttggagt atcacgaagt aattcatgga caaacattgt aaataaagag
    16621 actcgattag aaatagtaaa taagggtata aatggtgata caacaagtgg tatgttagct
    16681 aggtttaatg aagatgttgt aaaaaactct cctgatatag tttttataat gggtggcacc
    16741 aatgatttta ttgctggtgc gagaaatgaa attattaatt ctaatataat ggctatggtg
    16801 catcagtcat attcaaaaaa tatcatacct ataataggta taccattaaa accagatatt
    16861 ccaaatgtta gggcagattg gagttgtttt actgatttta atatagtatc tcgaaactta
    16921 gaatcatata gttattggat aaaaaaattt tgtatgacat ttaatactaa tttcgtagat
    16981 ttttattcag aatataataa aaatatggaa ctagaaggat ataaaaaatt atattttgat
    17041 gggttacatc caaccaagga aggtcataga ataatggcag acatttttat taattcaatt
    17101 aataagtata tagaagagtt atagattaca ttaaaatgct tgtttatgtg tcagttgcaa
    17161 tgaatacata cagtagagag gggtttatta tggattatac taaaacttat gaagagtgga
    17221 caaagggttc atattttgat gaagatacaa agctagagtt ggagaagata aaaaataatg
    17281 aaaaagagat agaggataga ttttataagg atttagaatt tgggactgca ggtcttagag
    17341 gaataattga agcaggaacg aatagaataa ataagtacac agttagaaga gctacttttg
    17401 gattggcaaa ctatatatta gaaaacacaa caaaagaaga aacaagcaga ggagtagtaa
    17461 tagctcatga taatagacac aagtctagac aattttgtat agaatctgca aatactttgg
    17521 cagcatgtgg tataaaagca tatatatttg atagtttgag aactactcca gaattatctt
    17581 ttgctgttag aagcttaaat gcaattgctg gtatagtcat aacagcaagc cataatcctc
    17641 cagaatataa tggatataag gtatactggg aagatggagc acaagttatg ccagagattg
    17701 caaatgcaat aacagagaaa gtaaatagta tacatgatta tagtacaata ccaaccttaa
    17761 caggggccaa taaaaatttg gtggttttgt tggatgaatc acaagatact aaatttatag
    17821 aagcagtaaa aagccaaatt ataagaaaag atttagttaa aaatgtaggt aaaagcttta
    17881 agatagtcta tacgcctctt tgtggaacag ggaatattcc aataagaaga gcattgaaag
    17941 aagttggttt tgaaaatata attgtagttc cagaagaaga aaatccagac ccaaattttg
    18001 cagggcttga ttatccaaat ccagaagaga aaaaagcttt aaatagaggt atattactcg
    18061 caaaagaaaa aggagcagac cttgtaatag caactgaccc agattgtgat agagtaggtg
    18121 tagcagtaaa aactactaca ggtgagtatg ctttacttac aggaaatcaa attggtggta
    18181 tgctgacaca ttatataata gaaggattaa aagaaaataa taagttaaaa gaaaatccta
    18241 caataataaa aaccatagtt acgtcagaat ttggagcaga tatagctaaa tcaaataatg
    18301 ttgatgtatt aaatgttctt acaggcttta aatttattgg tgaaaaaata aaattatttg
    18361 aacaaaataa aaatagaagt tatgtatttg gatatgaaga aagttatggt tacttagttg
    18421 ggactcatgc tagagataaa gatggtgtag tttcttcatt gttaatatct gaaatggcag
    18481 cattttacta ttcaaaaggt atgagtttat atgatggttt aatagaactt tataaaaagt
    18541 atggattttt caaagaacag actatatctt tgactttaaa aggtatagaa ggtgtagaaa
    18601 agataaaaga aataatatct tactttagag aaaatcaaat tgattatata aatagtatta
    18661 aagtagttga taaaaaagat tataaaaatg gtatagataa tcttcctaaa tctgatgttt
    18721 taaaatactt tttggaagat gaatcttggg tagctataag accatctgga acagaaccaa
    18781 aacttaaatt ttacatagct gtaaaaggag ctagtgatat tgaagcagat aaaaagattc
    18841 aagggttgaa aaaatatata gatgacatgg tcgaaaaatt aaaataaaat attattattt
    18901 tataagagaa ttttgtaata atatttattt agctgaaaat tataatttac
//
LOCUS       DAEPXF010000069        18693 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain HB27
            SAMN13376389-rid12479423.denovo.073, whole genome shotgun sequence.
ACCESSION   DAEPXF010000069 DAEPXF010000000
VERSION     DAEPXF010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13376389
            Sequence Read Archive: SRR10518432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 18693)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18693)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001069624.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001069624.1
            Genome Coverage       :: 315x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/15/2021 01:54:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,165
            CDSs (total)                      :: 4,076
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 89
            rRNAs                             :: 10, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 10 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 19 of 69
            Pseudo Genes (incomplete)         :: 44 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 10 of 69
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18693
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13376389-rid12479423.denovo.073"
                     /strain="HB27"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="China: Hebei"
                     /collection_date="2017"
     gene            complement(307..1374)
                     /gene="rsgA"
                     /locus_tag="KRL67_002047"
     CDS             complement(307..1374)
                     /gene="rsgA"
                     /locus_tag="KRL67_002047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HBH1522924.1"
                     /translation="MNGMKQINLQDYGLNRNFITQSMEYHDLYLARIISQEKNFYQAV
                     CVNGVLFAEVSGKLRFEATIQSDFPTVGDFVMLDRCNNKNGNAIIHHVLHRKSLFVRK
                     ASGKVNEEQVVAANIDIVFLCMSLNKDFNIHRLERYLSLTWESGAIPVVVLTKSDLCD
                     NVHRKLQVVNSIALGVDVVVTSSIEPDGYKQISKYIKPGCTVALLGSSGVGKSSLINC
                     LLGGEFLNTKEIRIDDDKGRHATTRRELFLISGGGIVIDTPGMRELGMWDVSEGLDKS
                     FADIEAYFGKCHFRNCTHTSEPGCAIWEAIAHQELSPKRWESYRRLKTEAVFADNKSE
                     YLAKKEKKFKNISKINKKIRR"
     gene            complement(1371..1772)
                     /locus_tag="KRL67_002048"
     CDS             complement(1371..1772)
                     /locus_tag="KRL67_002048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNHCP domain-containing protein"
                     /protein_id="HBH1522925.1"
                     /translation="MNYKNRKRAYYQENSCMDNFICKNCGYLVVPEGAGTQHRNHCPH
                     CLHSLHVDIIPGDRAADCDANMEPIGVWVRKNGEWAIIHRCTRCGHLSSNRIAADDNS
                     VKLMSIALKPLSQPPFPLERIIEKLEKEETE"
     regulatory      2568..2664
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            2718..2894
                     /locus_tag="KRL67_002049"
     CDS             2718..2894
                     /locus_tag="KRL67_002049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH1522926.1"
                     /translation="MFRDKMDRCTHMLTAYIGSSYDYCDFIDTQLDDFILEYGENVVE
                     SCLHQVMVLVSKYN"
     gene            3221..3718
                     /locus_tag="KRL67_002050"
     CDS             3221..3718
                     /locus_tag="KRL67_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH1522927.1"
                     /translation="MKKRKIVIITLLLIGVLLLGGILYNLFLVKTANISMLKESWNLD
                     IPIPNKEIEVFDTQDSINGDGQSYFIQEFSEKNFKKVFNLKGGIVVSKDNINEIEKYI
                     DKFKRDSVNINKSNKNKIEEDFKKYKLEVKKDDKYIYKRNYENYVVLIIKKDEQKLYS
                     LIWNQ"
     gene            complement(4196..5554)
                     /gene="rlmD"
                     /locus_tag="KRL67_002051"
     CDS             complement(4196..5554)
                     /gene="rlmD"
                     /locus_tag="KRL67_002051"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HBH1522928.1"
                     /translation="MLSKNEMYIVDIVDIGQGGVGVGKYNGITVFVEGGLIQDKVKVR
                     INKVKKNYAVGDIVEIIEKSPFRVKRVCSDNLRDCGGCQIQDLDYNKQLEMKTNEVKQ
                     VLSRIGKLEDVEVHDTIGMDNPYRYRNKAQFPIQKNNGIPIIGFYKKKTHDVISTEKC
                     VIQHEINDKIVKIIKTYIRAYKVSIYDEKTHTGLIRHLVTKIGFTTKEVMVVLVANGN
                     KLPYLKELASVLKENIPGFKTLVVNVNQKNTNAILGRENIVIYGDGKINDYIGDLVFE
                     ISPLSFFQVNPVQTKVLYDKALEYANLNDTDTVFDIYCGIGTISLFLAQKAKKVYGIE
                     IIDDAIKDAKINAKLNNIHNAEFYVGKAEEIVPKMYREGKTANVVVVDPPRKGCDEKV
                     LETIVSMNPERIVYVSCNPSTLARDLDYLNVHGFKCVEAQPVDMFPHSTHVETVAKII
                     KQ"
     gene            complement(5729..7489)
                     /gene="pyk"
                     /locus_tag="KRL67_002052"
     CDS             complement(5729..7489)
                     /gene="pyk"
                     /locus_tag="KRL67_002052"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HBH1522929.1"
                     /translation="MLNNVKKTKIVCTLGPASQSEEVLTQLMQNGLNVCRFNFSHGSH
                     EEHKERIDMAKKVREKLNKPVAILLDTKGPEIRTGNFEDPEVFLEEGQKFTITMKDIM
                     GTKEMCTVSYKGLAEDVKSGDSILIDDGLVGLRVKEINGEDIICVVENSGIVKNHKGV
                     NVPGVKINLPAITPKDISDIEFGISQGIDYIAASFVRKASDVLAIREVLENNNATDIQ
                     IISKIENQEGVENLDEILKVSDGIMVARGDLGVEIPTEEMPIVQKMMIKKCNELAKPV
                     VTATQMLDSMIRNPRPTRAEVTDVANAIYDGTDAIMLSGETAAGKYPVEAVKMMATIA
                     KRTEETLDYDRLLKENGTNNVTVTDAISHATCTTAVDLNASAIITSTSSGYTARMVSK
                     FRPKSPIIATTNNEKTMNKLALTWGVYPVKSSVAGNTDEVIEKAIEAARQADYIDNGE
                     LVVITAGVPVGVSGTTNLIKVHVISEEIVQGIGVGTQTVEGKVRIIKNGDACVDFNEG
                     DILVSTATDAEMNNYIEKAGAVVTENGGMTSHTAIVGINLDIPVIVSATDITNLVKDG
                     EVVTVDASRGVVYRGSTRVL"
     gene            complement(7541..8500)
                     /gene="pfkA"
                     /locus_tag="KRL67_002053"
     CDS             complement(7541..8500)
                     /gene="pfkA"
                     /locus_tag="KRL67_002053"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HBH1522930.1"
                     /translation="MKTIGLLTSGGDAPGMNAAIRAVVRSAIYYGCKVYGINRGYKGL
                     LEEDLTEMNLSSVGDIIHRGGTILKSSRCEEFKTEEGRLKAVKILKKYKIDCLVVIGG
                     DGSFAGAQKLSDLGFPAIGIPGTIDNDLAYTDYTIGFDTAMNTIIDAIGKIRDTSSSH
                     ERVNIVEVMGRHCGDLALYAGLAGGAETIIVPEVEITVDEVALRLKTTQKRGKRHSII
                     VLAEGVGSASDLEKELKKESGADLRVTVLGHVQRGGSPTVSDRILASRLGVRAVELLL
                     DGKSARVVGIKENKIIDLEISEALAQKKVFDKEAYEMAKILSI"
     gene            complement(8709..12278)
                     /locus_tag="KRL67_002054"
     CDS             complement(8709..12278)
                     /locus_tag="KRL67_002054"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HBH1522931.1"
                     /translation="MNKDFAHLHVHTEYSLLDGFSRVKKLIKRAKELNMSSIAITDHG
                     CMFGVIDFYKTAIKEGIKPIIGCEVYTAARGLRDKDPNYDKYQGHLVLLAKNMEGYKN
                     LIKIVSTSYVEGFYYKPRVDMEELKKHNKGIIALSACLAGDVARALMNRNYEKAKQFA
                     IDYRDIFGEENFFLEIQDHNLPEQKEVNSGLVKLSKETGIPLVATNDVHYVNKEDSKI
                     HDVLMCIQMGKTLNDPNRMRFGSDEFYLKSREEMEELFPYALEAIDNTVKIADMCNVE
                     FDFNTIHLPKYDVPEGYTPETYLRELCFNGLKERYDNPSNEILERLNYELDVIEKMGY
                     VEYFLIVWDFINFSKENNIIVGPGRGSAAGSIVAYTLKITDIDPIKYSLLFERFLNPE
                     RISMPDIDIDFCYERREEVIDYVKRKYGDDHVAQIITFGTMGAKAAIRDVGRVLDIGY
                     NKVDKVAKEIPFALGMTIDKALDTNPNLKELYDQDPETKEIINISKQIEGMLRHASTH
                     AAGVVISKNPVDEYVPLYKHQDAITTQFTMTTLEELGLLKMDFLGLRTLTVIRDTLDL
                     IEKNRDIKNYTEKIDFSKMDYDDPKVYEMLSSGNTLGVFQLESAGMRSFMKQLKPDNF
                     EDIVAGISLFRPGPMDSIPAYIKNKSNPKDVTYLHEKLKPIMEVTYGCLVYQEQVMQV
                     VRDLGGYSYGRSDLVRRAMSKKKMDVMEEERQYFIHGKFDEDGNIEIAGCIRNGVEEE
                     IANKIFDDMIDFARYAFNKSHAAAYGVLAYETAYLKTYYPVEFMAALITSVMGNTDKV
                     VEYIRECKALEIDVLPPDINKSFSKFSVEGDNIRFGLAAVKNVGVNIINNIIAERESN
                     GLFVDLVDLVKRLDQKDTNKRVIESLIKCGAFDNISENRASLMAGYESLLESVSMDRK
                     KNVQGQISLFDAFGDSEEDSDFQQIYSLPKRQEYEERERLNLEKEVLGMYVSGHPLSQ
                     FEKELQEKTSMDNGKLNSLKEEEESYLQMNETDVIMGGMIVTKTIKTTKRNEIMAFIE
                     LEDLYGAIEIIVFPQLLQKYNVILNEDNIIYVKGTLSIKEGENAKLIAREIKDINDDT
                     DFADRNYNSGRTRNVENIKPESGKKLYLKIDSMSDTDTTNNIVEISKQYPGNDSIYLY
                     PMDENINGKRKAYKMTGVLTCICDEFINNLEELLPKDNIKVKA"
     gene            complement(12429..13382)
                     /gene="whiA"
                     /locus_tag="KRL67_002055"
     CDS             complement(12429..13382)
                     /gene="whiA"
                     /locus_tag="KRL67_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HBH1522932.1"
                     /translation="MSFSTETKNELARVISGNECCNIAELSALVKSGGSIQIVGYKKL
                     NLKITTELNSIARKVFKLLKKNFNINTTISVNKNQMLKRNNSYVLMVTSEMGSEILLK
                     KLGILENKEGFFTINKISENLIKHEGCKRAFIRGAFLGGGSISDPEKNYHMEFVTNNE
                     EFADSLKELINSLGFNSKIVARKNNHVVYIKESEQISDLLSIIGGHHALLSLQNTKIV
                     KEMRNNVNRIVNCETANLSKTVNAAVRQVENIKLIQETIGISSLPENLQEIAKIRIEY
                     EDMTLKELGEMLEPPIGKSGVNHRLRKIEEIATDLKKKSSQ"
     gene            complement(13399..13824)
                     /locus_tag="KRL67_002056"
     CDS             complement(13399..13824)
                     /locus_tag="KRL67_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HBH1522933.1"
                     /translation="MREEVSAGGVVLFGNTILLLRKFNGDWVLPKGKVEEGENNQEAA
                     LREVSEETGVKADILKYLGEIHYTFKENWDENRAVHKTVFWYLMQAKNMDTIPQKEEG
                     FIDAKFIHLDRVVDLARYDDEKEIIKVALQEIKKRLKKN"
     gene            complement(13893..14999)
                     /locus_tag="KRL67_002057"
     CDS             complement(13893..14999)
                     /locus_tag="KRL67_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YvcK family protein"
                     /protein_id="HBH1522934.1"
                     /translation="MAKLLIIVGILGWIALIVSLFIYKKAKNEKVKLFQRSIVENREP
                     NVVVIGGGTGQSVFLRGLKHTTQNITAIVTVADDGGGSGVLREDLGMLPPGDIRNCLL
                     ALANIEPTMNEVMQYRFTEGLLKGQSFGNLFLAAMNGLYGNFEKAVYKLSEIFAITGR
                     VLPVTLEDVNLVAKLENGNIINGESSIPEESKIQKSSIDKIFLNPKDVKPLKDVIASI
                     YDADIIIMGPGSLYTSIIPNLLVEGIVDAIKSSVAPKVYIANIMTQPGETEGYNVLEH
                     VNAIVKHTDENLIDYVIANNEILPEEMLDLYKQDGAEQVLLDKKQKDKLKEMGIKTVE
                     KNLIEIKNNYIRHDAKYISNIVIELALNHNYNKS"
     gene            complement(15011..15868)
                     /gene="rapZ"
                     /locus_tag="KRL67_002058"
     CDS             complement(15011..15868)
                     /gene="rapZ"
                     /locus_tag="KRL67_002058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HBH1522935.1"
                     /translation="MKFVIVTGLSGSGKSETMRALEDMGFYCVDNLPPALITKFAELC
                     YQPNSSIDKVALGIDIRGRKFFEALHESLNYLEKENYEYEMVYLDCNDDVLLKRYKMT
                     RRNHPLAKDMQIPEGIKMERKIMEPLKELSTCIIDTTNMKPKDLKEEIKKIYSSGEDN
                     PNLTISVVSFGFKHGILADADLVFDVRFLPNPYYVEELRAKTGDDKEVRDYVMNSKIS
                     EEFYVKLLDMIHFLVPQYIDEGKQHLVIGVGCTGGRHRSVTITNLIAEDLSNKGYRVV
                     KKHRDSMLR"
     gene            complement(15899..16612)
                     /locus_tag="KRL67_002059"
     CDS             complement(15899..16612)
                     /locus_tag="KRL67_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HBH1522936.1"
                     /translation="MKILADYHTHTLYSHGKGTIEDNVKVAISKGIKTIGISDHSYKH
                     IAYGVKKKDILKMREEVDYLNDKYNDINILLGMECNILDDKGNIDMEDKFIRYFDYIM
                     AGYHFGSTPTSLRSLLNHCNNYILKTEKAKEYNTKAVINAISNNDIFIITHPGDKGDV
                     YIEEVAKTAKKTNTRLEINSSHSFLNVNQLNKIKGLENKFIIGSDAHCPERVGDFKLA
                     MKTISDSNLDISLVENIII"
     gene            complement(16616..17530)
                     /gene="murB"
                     /locus_tag="KRL67_002060"
     CDS             complement(16616..17530)
                     /gene="murB"
                     /locus_tag="KRL67_002060"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HBH1522937.1"
                     /translation="MNNQDIYINLLNILSKEDIKVDEPMKKHISFRVGGPADILVRPR
                     TEEQLKNVLKLVKEESIPYLIIGNGSNILIKDGGIRGVVIELADNFNSYEINDTRMTA
                     QSGALLSVLGKALQKQELKGFEFASGIPGTLGGALAMNAGAYGGEMKDIVKSVRLMDM
                     EGNIFELSNEQMEFGYRKSIISKNGYIALSAELELQEGNYDEIKSLMDDLATRRITKQ
                     PLNFASAGSTFKRPTGYFAGKLIEETGLRGLTLRGAQVSEKHCGFVVNQGEASAKDIL
                     DLIYVIKSAVYAKFGVMLEEEVKILGED"
     gene            complement(17803..18573)
                     /locus_tag="KRL67_002061"
     CDS             complement(17803..18573)
                     /locus_tag="KRL67_002061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HBH1522938.1"
                     /translation="MSKGFLTPGETAQATISAGIKKANISTQNAILLGLFGGAFIGFG
                     ALGSMIVSQTFGKIDPGVASFLSAMVFPVGLMLVVVAGAELFTGNTLMAIALMDKKIT
                     LGKMLRNWGLVYFANLVGSILLVALVFYSSTLTGDAATKAIAVAEAKVNSTIASMFLK
                     AILCNILVVLAVWMATASQDIMSKLASCWTVIMLFVLCGFQHSVANMFFIPMGMALGA
                     NITMVQFITNLVFVTLGNIVGGSIVVGGIYYLCYVKNS"
BASE COUNT         5883 a         3490 c         2076 g         7244 t
ORIGIN      
        1 tttactttta agaaacacct ctatcataca taagcatata ctctattatg tattagtata
       61 aatatgcctt ttcttatctt acataagacc gctttataaa agagcctatc ataacaaata
      121 tgtatacgtt atcatatgta tatatcatta tataaaaaat caaactttat taaaaaaatt
      181 aataaattgc cttaaataat accacaaacc aaatttgcca tcaaaatcac attaaagtaa
      241 caaattgatg gcaaatttaa tatattaata gtatgtagac tcatataatg aaaaaaaaaa
      301 ctacacttat cttctaatct tcttatttat ttttgagata tttttaaatt tcttttcttt
      361 ttttgctaag tattcgcttt tattgtctgc aaatactgct tcagttttaa gcctacgata
      421 ggattcccat cttttaggcg aaagttcttg atgagcaata gcttcccaaa tagcacatcc
      481 tggttctgag gtgtgagtac aattacgaaa atgacatttt ccgaaataag cttctatatc
      541 tgcaaaactt ttatctaaac cttctgaaac atcccacatt cccaactctc tcatacctgg
      601 tgtgtcaata acaataccac cacctgaaat taaaaatagc tctcgtctag tcgtggcatg
      661 tcttccttta tcatcatcta tgcgaatttc ctttgtattt aaaaattctc cacccagcag
      721 acaattaatt aatgaagatt ttccaactcc tgaagaacca agtaaggcaa cagtacagcc
      781 tggtttaata tacttagaaa tctgtttata accatctggc tctatagatg aggtaacaac
      841 tacatctaca cccaaggcta tagaattaac tacttgtaat ttacgatgca cattgtcaca
      901 taagtctgat ttagtaagta ctactactgg gatagctcca ctttcccatg ttaaagacag
      961 atacctctcc aatctatgaa tgttaaaatc cttattcagt gacatgcaca aaaaaacaat
     1021 gtcaatatta gctgcaacca cctgttcctc atttaccttt ccagaagcct tacgcacaaa
     1081 caagcttttt ctatgaagca catgatgaat aatagcatta ccatttttat tattgcaacg
     1141 atccaacata acgaaatcac caactgtggg aaaatcagat tgtatagtag cctcaaaacg
     1201 tagttttcct gaaacttctg caaacagtac accatttaca catacagctt gataaaaatt
     1261 cttttcctgc gagataattc gagcaaggta taaatcatga tattccatac tttgagtaat
     1321 aaaattcctg tttaggccat agtcttgcaa attgatttgt ttcataccat tcattctgtt
     1381 tcctcctttt ccaacttttc aatgattctt tctagtggga aagggggctg tgacagtggt
     1441 tttaatgcaa ttgacattaa tttcactgaa ttatcatcag ctgcaatacg attagaacta
     1501 agatgtccac agcgtgtaca acgatgaata attgcccact caccattttt tctcacccac
     1561 actccaatag gttccatatt agcatcacaa tctgcagcac ggtcaccagg aatgatatct
     1621 acatgaagac tatgtaaaca atgtggacaa tgatttctat gttgtgtacc agctccttca
     1681 ggaactacaa ggtaaccaca atttttgcaa ataaagttat ccatacagga attttcctga
     1741 taataagctc gttttctatt tttataattc atttttattt cctccgtatc ttgtattttc
     1801 tacaaaaaaa gtccacaggt atttatatct gcctttttat gtggtataca tatttaccta
     1861 cacaaaaaca gaaaactcct gtggactaga aaatttaata tcttctattt caacataatt
     1921 atattcaaaa taatactgtg taaaattaat aaaagtgtag cagattaaag cccactacac
     1981 cacaatttaa caaagtaata tgtaatatta ttatttgttg tctaatgtta gttatctata
     2041 aaggcagagt aagacacaac aatcaaatgt atcaaaaata cacttaaaaa ttctgattaa
     2101 acagaaccca tatgcgtctc caactattta tttgttggtt accattatat ataactctga
     2161 catcaagcaa aacactcctt tactaataat tattattgaa attttaccat aaaatataag
     2221 tatgttcaag tataatttaa tatattcata tatttatgtt tgtctcattt tcttattcta
     2281 taaaatctag tggtatttta aaatttaatg tttacctagt attaataata aaaattaact
     2341 gctattattt gttttctcat tttatgctta tcgctcaatt tatctaactt aaattaatag
     2401 caaaggtctt ttaatacaaa acctacaatc aagtttttcc atacattcta catacatagc
     2461 ctattttttt gatataaaat aacgttcata tttataaatt catttaattt tcaaataagt
     2521 atattgaata ataagcaatt ctattgtatt attgtaattg tagaataaaa ctaaatcggc
     2581 aaaactagag aaatttagtg acgcaaagct atagggacta acgcttataa aataaactat
     2641 gagttatgtc agccagttgc caaaaagaga tattcttttt gtttttatga aagtttttta
     2701 gggggataat aatatatatg tttagagata aaatggatag atgtacacat atgttgactg
     2761 cttatattgg tagttcatat gattattgtg attttataga tacacaacta gatgatttta
     2821 tactagagta cggagaaaat gtggtagaat cttgtttgca tcaggtgatg gtattagtaa
     2881 gtaagtataa ttaaatagat ttggttttct agagaaccct ttttagtttt atctacttta
     2941 aatagtctta tgttagaaag ctaattaaga tagatttcta gtattcataa ttttattttt
     3001 tatttaaaat acatataata aagatgtaaa tttgtatcaa agaataaata tgagaactta
     3061 aattctctta tctatatgaa aataaacatc tcttaaaatg aaattctgta aacataaatg
     3121 atttttaagt ttgaaggatt agattaacaa aattattctt gatgacatac cttcaaatga
     3181 tgaattaaat catgtttttt caaaaggttg tgaacgaaaa atgaaaaaaa gaaaaattgt
     3241 aataatcaca ttactattaa taggtgtttt gttattagga ggtatcctat ataatctttt
     3301 tttagtaaaa acagctaata tttcaatgct aaaggaatct tggaatttgg atataccaat
     3361 acctaacaaa gaaattgaag tatttgatac tcaagatagt attaatggag atggacaaag
     3421 ttattttatt caagaatttt ctgaaaaaaa tttcaaaaaa gtttttaatt taaaaggtgg
     3481 tatagttgta agcaaagata atattaatga aatagaaaaa tatattgata aatttaaaag
     3541 agattctgtt aacattaaca aaagtaataa aaataaaatt gaagaagatt ttaaaaaata
     3601 taagttagaa gttaaaaaag atgataagta tatatataag agaaattatg aaaactatgt
     3661 agttttaatt ataaaaaaag atgaacaaaa attatacagt ttaatatgga atcagtaggt
     3721 acatatgtat tattaagaat tataaataat aaaaacaaat tttagtcaat atacaaaaac
     3781 cttttaaatt gtctggaaat cacctcttca tttgttataa aaacatacaa caaatgaaga
     3841 ggtgatttta tggtttttaa aaaaataatt ataagtggat atgtgctttc acatgtttat
     3901 caacatctgt aatgcctata tttactagtg aattataata taaaaatatt aaataatatt
     3961 caactataga ttatgtaaat tattctatgg aaaaaatgag ttgtataaaa tctggcaact
     4021 gtagattgac gatttctttt aaaattacaa ttataataag ttctatagaa ttaaaaataa
     4081 aactttagag atttttgata acattgtaga ctaaatttgc caccaaaatt actttacagt
     4141 ggcaaatcgg tggcaaatac aatatattat tagtatataa tctatataaa cattactatt
     4201 gtttgattat cttagctaca gtttccacat gtgtagaatg agggaacata tctacaggtt
     4261 gtgcctcaac acatttaaat ccgtgaacat ttagataatc taagtccctt gctaaagtag
     4321 aaggattaca agaaacatag acaattcttt ccggattcat tgaaacaata gtctctaata
     4381 ccttttcgtc acatcccttt ctaggtgggt ctacaactac tacattggca gtcttaccct
     4441 ctctatacat cttaggtaca atttcctctg cctttccaac ataaaattca gcattatgta
     4501 tattgtttaa tttagcattt atcttagcat cttttatggc atcatctata atttctattc
     4561 cataaacctt tttagccttt tgtgccaaaa ataaagatat tgtacctata ccacagtaaa
     4621 tatcaaaaac agtatcagta tcatttaaat ttgcatattc aagagcctta tcatacaata
     4681 ctttagtttg aacagggttt acttggaaaa atgataatgg tgaaatctca aacactaagt
     4741 cacctatata atcgtttatt tttccatctc catatataac tatattttct cttcccagaa
     4801 tagcattcgt atttttttga tttacattta caaccaaagt cttaaatcca ggtatatttt
     4861 ccttaagcac tgaagctaat tctttaagat atggaagttt atttccattc gctactaata
     4921 caaccattac ttctttagtc gtaaaaccta tttttgttac taaatgccta atcaaccctg
     4981 tgtgagtctt ttcatcatat atacttactt tataggccct tatatatgtt tttattattt
     5041 taacaatttt atcatttatc tcatgttgta ttacgcattt ttctgtcgat attacatcat
     5101 gagtcttttt tttataaaag ccaataattg gaataccatt attcttttgt attggaaatt
     5161 gtgctttatt tctatatcta tatggattat ccattcctat agtgtcatga acttctacat
     5221 cctctaactt acctattctc gaaagtactt gtttaacttc atttgttttc atttcaagtt
     5281 gtttattata atctaaatct tgtatctgac accctccaca atccctaaga ttgtcactac
     5341 acactctttt aaccctgaat ggagactttt ctattatctc aacaatatct cctacagcat
     5401 aatttttctt tactttatta atcctaactt taactttatc ttgtattaat ccgccctcta
     5461 caaaaactgt aataccattg tactttccta ctcctactcc gccttgccca atgtctacta
     5521 tatctacaat atacatctcg tttttcgaaa gcataaatca ccctccaatc aatctattat
     5581 atttaaatct ttacaaactt ttttctttaa atttatatta aaatattgct attaattaaa
     5641 ctatattttt tactaagcta caatatttta ttttaccaaa aaaaaaggag cttataaagc
     5701 cccttagtaa ataaatttat tatttttctt ataaaaccct agttgaacct ctatatacca
     5761 cacctcttga tgcatccaca gttacaactt ctccatcttt tactagattt gttatatcag
     5821 ttgcagacac aataactggt atatctaaat ttatacctac aatagctgta tgtgatgtca
     5881 ttccaccatt ctcagttact acagctcctg ctttttctat ataattgttc atttcagcat
     5941 cagtagcagt agaaacaagt atatctcctt cattaaagtc tacacaagca tctccattct
     6001 ttattatacg aactttacct tctactgttt gagtacctac acctatacct tgcacgattt
     6061 cttcgcttat tacatgaacc tttattaaat tagtagttcc acttactcct actggtactc
     6121 cagcagttat aactactaat tctccattat ctatataatc agcttgtcta gctgcctcaa
     6181 ttgccttttc tataacttca tctgtatttc ctgcaacaga agacttaact ggatatactc
     6241 cccatgtaag agctaattta ttcatagttt tttcattatt tgtagtagct attattggag
     6301 attttggtct aaatttagat accattctag cagtatatcc tgaagaagta gatgttatta
     6361 tagctgatgc atttaaatct actgctgttg tacaagttgc atggcttatt gcatctgtta
     6421 cagtaacatt gttagtacca ttttccttta ataatctatc ataatctaaa gtctcttctg
     6481 ttctttttgc tattgtagcc atcattttta ctgcctctac aggatatttt ccagcagcag
     6541 tttctccaga taacattata gcatctgttc catcatatat agcattggca acgtctgtaa
     6601 cttctgctct tgttggtctt ggatttctta tcatagaatc aagcatttga gtggcagtga
     6661 caactggctt tgctaattca ttacattttt ttatcatcat tttttgaact attggcattt
     6721 cttcagttgg tatctcaacg cctaagtcac ctctagctac cattatacca tcagaaactt
     6781 ttaatatttc atctaagttc tctacacctt cttggttttc tattttagat attatttgta
     6841 tatcagttgc attattgttc tctaatactt ctcttattgc tagcacatca gatgcctttc
     6901 ttacaaaaga agcagctata taatctattc cttgacttat cccaaactct atatcactta
     6961 tatctttagg agttattgct ggtaagttta tcttaacacc aggtacattt actcctttat
     7021 gatttttaac tattcctgaa ttttcaacaa cacaaattat atcttctcca tttatttctt
     7081 ttactcttaa gccaactaaa ccatcatcta ttagtataga atctcctgat ttaacgtctt
     7141 cagcaagacc tttatagctt actgtacaca tttctttagt tcccataata tctttcatag
     7201 ttatagtgaa cttttgccct tcttctaaaa atacctctgg gtcttcaaag ttacctgttc
     7261 taatttctgg acctttagta tctaataata tagcaactgg cttatttaat ttttctctaa
     7321 ctttctttgc catatcaatt ctttctttat gttcttcatg cgaaccatga gaaaagttaa
     7381 atctacacac atttagacca ttttgcataa gttgagttaa aacttcctca ctttgtgatg
     7441 ctggacctag tgtacaaaca atttttgtct tctttacatt atttaacata tttgctcctc
     7501 ctgagatatt attccacaga tttattttta atactaaaac ttatatagat aatatttttg
     7561 ccatctcata agcttcttta tcaaatactt tcttttgagc taaagcttca cttatttcta
     7621 aatcaattat tttgttttct tttattccta caactctagc tgattttcca tctaataata
     7681 attctactgc tcttactcca agtctacttg ctagtattct atcagataca gttgggcttc
     7741 cgcctctttg aacatgcccc aatacagtta ctcttaaatc tgctcctgac tcttttttaa
     7801 gttccttttc taaatcgcta gcacttccga ctccttctgc taaaacgatt atactatgtc
     7861 tcttacctct tttttgagta gtctttaatc ttagagcaac ttcatctact gtaatttcta
     7921 cttctggtac tattatagtt tccgctcctc ctgcaagacc tgcatataag gctaaatctc
     7981 cacagtgtct tcccataact tctactatat taactctttc atgagatgat gatgtatctc
     8041 ttatcttacc tatagcatct ataatagtat tcattgcagt atcaaatcct atagtataat
     8101 ctgtatatgc taaatcgtta tctatagttc ctggtatacc tatagctggg aatcctaagt
     8161 cactaagttt ttgagctcct gcaaatgaac catctccacc aattacaact aagcaatcaa
     8221 ttttatactt tttaagaatt tttactgctt ttaatctacc ttcttctgtt ttaaattctt
     8281 cacatctaga agattttaat atagttcctc ccctgtgtat aatatctcca acagaagata
     8341 agttcatttc agttaaatct tcttctagta atcccttata ccctctattg attccatata
     8401 ctttgcatcc ataatatatt gctgatctta caaccgctct aatcgcagca ttcattccgg
     8461 gagcatctcc tccacttgtt agtaatccta tagttttcat tttttacctc cttggatttt
     8521 atgtcaattt tttattataa tcattgttat tgtattctaa tggacttttt ttgtccctat
     8581 atcacttttt aatcccatca agactaaaaa aattaaatgt ccttaaatgt ccattttgtg
     8641 atattatatc acaaaacgga caatattttt catattatta taatatattt agatttttat
     8701 ataaaaaatc aagcttttac ttttatgtta tctttaggaa gtaactcttc taagttattt
     8761 ataaattcat cacatataca agttaatacc ccggtcattt tatatgcttt acgtttacca
     8821 tttatatttt catccatagg gtacaagtat atactgtcat tacctggata ttgtttggat
     8881 atttctacaa tattattagt tgtatcggta tcagacatac tatctatttt caaatacaat
     8941 tttttaccac tttctggctt aatattttct acatttctag ttcttcctga attgtagttt
     9001 ctatctgcaa agtcagtatc atcatttata tcttttatct ctctagctat aagttttgca
     9061 ttttcgcctt cttttatact taatgttcct tttacatata ttatattatc ttcattcaaa
     9121 atgacattat acttttgtaa taattgagga aaaactatta tttctatagc accatacaaa
     9181 tcttctaatt caataaatgc cataatttca tttcgttttg ttgttttaat agttttagtt
     9241 acaatcattc cacccattat aacatctgtt tcattcattt gtaggtatga ctcttcttct
     9301 tcttttaatg aatttaattt tccattatcc atagatgttt tttcttgtaa ttctttttca
     9361 aattgtgata aaggatgtcc acttacatac attcccaata cttctttttc aaggttaagt
     9421 ctctctcttt cttcgtactc ttgcctttta ggcaaactgt atatttgttg aaaatcacta
     9481 tcttcctcac tatctccaaa agcatcaaat aaagaaattt gaccttgaac atttttcttt
     9541 ctatccatag atacactttc taataaactt tcataaccag ccatcaaact agctctattt
     9601 tcacttatat tatcaaaagc tccacatttt attagacttt ctattactct cttgtttgta
     9661 tctttttggt ctaatctttt gactaaatct actaaatcta caaataatcc attagattct
     9721 ctttcagcga ttatattgtt tattatattt acgccaacat tttttacagc tgctagacca
     9781 aatcttatat tgtcaccttc aactgaaaac tttgaaaaac ttttgtttat atctggaggt
     9841 aatacatcta tttctagtgc tttacattct cttatatact caactacctt atcagtattt
     9901 cccattacac tggttatcaa tgctgccata aattcaactg gataatatgt ttttagataa
     9961 gctgtctcat atgctaaaac cccatatgca gctgcatgtg atttattaaa tgcatatcga
    10021 gcgaagtcta tcatgtcatc aaatatttta ttagctattt cctcttctac accatttctg
    10081 atacaccctg caatttctat attaccatct tcatcaaatt taccatgaat aaaatattgt
    10141 ctttcttctt ccatcacgtc catctttttc ttactcatgg ctcttctaac taggtcactt
    10201 cgtccataac tataaccacc taaatctcta acaacctgca taacctgctc ttgatatacc
    10261 aaacaaccat atgtaacttc cattataggc tttaattttt catgtagata tgttacatct
    10321 tttgggttgc ttttattttt tatatatgca ggtatagaat ccattggacc tggtctaaat
    10381 agggatattc ccgctacaat atcttcaaaa ttgtctggtt ttaattgttt cataaaactt
    10441 ctcataccag cactctccaa ctgaaacacc cctaatgtat ttccagatga aagcatttca
    10501 tatactttcg ggtcatcata atccatcttt gaaaagtcta ttttttctgt gtagtttttt
    10561 atatctctat ttttttctat taaatcaaga gtatctctta taactgtaag agttcttagc
    10621 ccaagaaaat ccatctttaa aagcccaagc tcttcaagtg ttgtcatagt aaactgtgtt
    10681 gtgattgcat cttgatgttt gtacaaaggc acatactcat ctacgggatt ttttgatata
    10741 acaacccctg ctgcatgagt agaagcatgt cttagcattc cctctatctg ttttgatata
    10801 tttattattt cttttgtttc tgggtcttgg tcatacaatt ctttaagatt aggatttgta
    10861 tctagggcct tatctattgt cattcctaaa gcaaatggta tttccttggc taccttgtca
    10921 actttattgt agcctatatc caatactctt ccaacatctc ttattgcagc ttttgcaccc
    10981 attgttccaa aagttattat ctgggctaca tggtcatcac catattttct ctttacataa
    11041 tctataactt cttctcttct ctcataacaa aaatctatat ctatatctgg catggatata
    11101 cgttctggat ttaaaaaacg ttcaaataat agagagtact ttattgggtc tatgtcagtt
    11161 atcttaagtg tatatgctac tatcgaacca gctgcactac ctctacctgg acctactata
    11221 atattatttt ctttagaaaa atttataaaa tcccaaacaa ttaaaaaata ctctacataa
    11281 cccatttttt caattacatc tagttcataa tttaatctct ctagtatctc attgctagga
    11341 ttatcatatc tctcttttaa tccattaaaa cacaactccc ttaaataagt ttctggtgta
    11401 tacccttctg gaacatcata cttaggaaga tgtatagtat taaaatcaaa ctccacatta
    11461 cacatatcag ctattttcac agtattgtca atagcctcta atgcatatgg aaataattct
    11521 tccatttctt ctcttgattt taaataaaac tcatcactac caaacctcat tctatttggg
    11581 tcatttaatg tttttcccat ttgaatacac ataaggacat catgtatttt tgaatcttct
    11641 ttatttacat aatgaacatc attagtagca actagtggaa taccagtctc ttttgataat
    11701 tttactaatc cagaatttac ttctttttgt tcaggtaaat tatggtcttg aatttccaga
    11761 aagaaatttt cttctccaaa tatatctctg tagtcaatag caaattgttt tgctttttca
    11821 taatttctat tcattaaagc acgtgctaca tctcctgcaa ggcatgcaga aagtgctata
    11881 atccctttgt tatgtttttt aagttcttcc atgtcaactc taggcttata gtaaaaacct
    11941 tctacgtatg aagttgaaac tatttttatt aaatttttat atccttccat attcttagct
    12001 aacagaacta aatgtccttg atatttgtca tagtttgggt ctttatctct caatccacgt
    12061 gcagctgtat agacttcgca ccctataatt ggctttattc cttcttttat agctgttttg
    12121 tagaaatcta taacgccaaa catacaacca tggtcagtta tagctattga gctcatgttt
    12181 agttccttag ccctttttat aagttttttc accctcgaaa acccatcaag taaactatac
    12241 tcagtatgta catgaagatg agcaaaatct ttgttcatta aatcacctcc tgctttataa
    12301 ttttaatttt accacaaata tatgtcctaa aacaaaaaca gagactttta tctcaaaata
    12361 ggaattaact tcaacatata ccattgtatg ttataaatta atgcttattc tgagattgtc
    12421 ccctcaattt attgcgatga tttttttttc aaatctgtcg caatttcttc tatttttcta
    12481 agtctatgat tgactccaga ttttcctatt ggcggttcta acatctctcc aagttctttg
    12541 agagtcatat cctcatactc gattctaatc ttagctattt cttgtaaatt ttctggcaaa
    12601 ctgctaattc ctatagtttc ttgtatgagc ttaatatttt ctacttgtct tactgctgca
    12661 tttactgttt tagaaaggtt agctgtctca caatttacta tcctgtttac attatttctc
    12721 atttctttta ctattttagt attttgaaga cttaaaagag cgtggtgtcc tcctataata
    12781 ctcaacaaat ctgatatttg ttcactctct ttgatataaa caacgtgatt attttttcta
    12841 gccacaattt tactattaaa acctaaagag tttataagtt cttttagaga atctgcaaac
    12901 tcctcattat tagttacaaa ttccatatga tagttctttt ctgggtcgct tatggaacct
    12961 ccaccaagaa aagctcctct tatgaaagct ctcttacatc cttcatgctt aattaagttc
    13021 tctgaaattt tgtttatagt aaaaaatcct tctttatttt caagtatacc taactttttt
    13081 aataatattt ctgaacccat ttcgcttgtt accattaata cataactgtt atttctcttt
    13141 agcatttgat tcttatttac tgagattgta gtattgatat taaaattctt ttttaataat
    13201 ttaaatactt tacgggctat tgaatttaac tctgttgtta tctttagatt taacttctta
    13261 tatccaacaa tttgtatact tccccctgat ttaactagag ctgacaattc agctatatta
    13321 caacattcat tcccagatat aactcttgct agttcatttt tggtttcagt tgaaaaagac
    13381 atatctatca cctattaact aatttttctt taaccttttt tttatttctt gtaaggcaac
    13441 ctttattatc tccttttcat catcatacct agctaggtct accactctat ctagatggat
    13501 aaatttagca tcaataaagc cttcctcttt ttgtggaata gtatccatat tctttgcttg
    13561 catcaaatac caaaaaactg ttttatgaac tgctctattt tcatcccagt tttctttgaa
    13621 tgtatagtgt atttcaccta aatatttcaa tatatctgct tttactccag tttcttcact
    13681 tacttcacgt aatgctgcct cttggttatt ttccccttct tctacttttc ccttgggtaa
    13741 cacccaatct ccattgaact tccttagtaa aagaatcgta ttgccaaaca ggactacacc
    13801 accagcacta acctcttctc tcatcgatgt tcactcctta aaactaacat aatgataagg
    13861 atttctacac aattttttaa aatccttttt tactaacttt tgttgtaatt atgatttagg
    13921 gctaactcaa taactatgtt agatatatac tttgcatcat gtcttatata attatttttt
    13981 atttcaatta aattcttctc tacggttttt atacccatct cttttaattt atctttttgc
    14041 tttttatcta acaagacttg ctctgctcca tcttgtttat ataagtcaag catttcttct
    14101 ggcaatatct cattatttgc gattacataa tctattaaat tctcatcagt gtgctttact
    14161 atagcattaa catgctctaa tacattgtac ccttctgtct cacctggttg agtcattata
    14221 tttgctatat atacttttgg agctacagac gatttaatag cgtctacaat cccctctaca
    14281 agtaaatttg gtattatact cgtatataag cttccagggc ccataattat aatgtcagca
    14341 tcgtaaatag atgcaataac atcctttaat ggtttgacat cttttggatt taaaaaaatt
    14401 ttatcaatag agcttttttg aatttttgac tcttcaggta tactagattc tccatttatt
    14461 atattgccat tttctaattt ggcaactaaa tttacatctt ccaaagttac tggtaaaact
    14521 cttcctgtta tagcaaaaat ttcacttaat ttatatacag ctttttcaaa gtttccatac
    14581 aacccattca tagcagccaa aaacaaattt ccaaaactct gaccttttaa aagcccttct
    14641 gtaaacctat attgcataac ttcattcatt gttggctcta tatttgcaag tgctaataga
    14701 cagtttctta tgtctcctgg tgggagcatt cctaagtctt ctctcaatac acctgaacct
    14761 ccaccatcat cagcaacagt tacaattgca gttatatttt gcgtagtgtg tttaagacct
    14821 cttaaaaata ctgattgacc agtacctcca cctatcacaa ccacatttgg ttctctattt
    14881 tcaacaatag acctttgaaa tagttttact ttttcatttt ttgctttttt atatataaat
    14941 aacgatacaa ttaatgcaat ccaacctaaa attcctacaa taataagtaa tttagccatg
    15001 tttgcctcca ttatcgcaac atagagtctc tatgtttctt tacaactcta tatcccttat
    15061 tagataaatc ctccgctata agatttgtta tagtaactga cctatgtctt ccacctgtgc
    15121 atccaacacc aattactaga tgttgttttc cttcatctat atattgaggt actaaaaaat
    15181 gaatcatatc taaaagtttc acataaaatt cttcactgat tttagaattc ataacataat
    15241 ctctaacttc tttatcatca ccagttttag ctcttaactc ttcaacataa tagggatttg
    15301 gaagaaatct tacatcgaat actaaatctg catcagcaag tataccatgt ttaaatccaa
    15361 aagatactac agaaatagtt aggtttggat tatcttcacc agaagaatat attttcttta
    15421 tttcttcctt aaggtcttta ggtttcatgt tagttgtatc aattatacat gtagataact
    15481 cctttaaagg ttccattatc tttctttcca tttttatacc ttctggaatc tgcatatctt
    15541 ttgccaatgg atgatttctt cttgtcattt tatatctctt taacaataca tcatcattac
    15601 agtccaaata taccatttca tattcataat tttctttttc taaataattt aagctttcat
    15661 gaagcgcttc aaaaaacttt ctccctctta tatctatccc tagagcaact ttatcaatac
    15721 ttgaatttgg ttgataacat aattctgcaa atttagttat caaagcagga ggtagattat
    15781 ctacacaata aaatcccata tcctctaaag ctctcattgt ctcacttttt ccagatcctg
    15841 aaagacctgt aactataaca aatttcattg ctaactcctc ccttttatct tatgtttatt
    15901 atattataat gttttctact aacgatatat ccagattaga gtcagatata gtcttcatag
    15961 ctagtttaaa atcaccaact ctttctggac aatgtgcatc tgagcctatt ataaatttat
    16021 tttctagccc ttttatttta tttaattgat ttacattcaa aaaagaatgg ctactattta
    16081 tttcaagtct agtatttgtc ttttttgctg tctttgctac ttcttctata tacacatcgc
    16141 ctttatctcc tggatgagta attataaaaa tatcattatt acttatcgca tttattacag
    16201 cttttgtatt gtattctttt gctttttcag tttttaaaat atagttatta caatgattta
    16261 ataagcttct taatgatgtt ggagtagaac caaaatgata tccagccata atataatcaa
    16321 aatatctaat aaatttatcc tccatatcta tattgccttt atcatctaat atgttacact
    16381 ccatacctaa aagtatattt atatcattgt acttatcatt taaataatct acttcttctc
    16441 tcattttgag aatatctttt ttctttacac catatgcaat atgtttataa ctatggtcag
    16501 atattcctat tgtttttatt cctttagaaa tggctacctt tacattatct tcaatagtcc
    16561 ctttgccatg actatataat gtatgtgtat gataatcagc cagtattttc atttgttaat
    16621 cctctccaag tattttgact tcttcttcca acataacccc aaacttggca tatacagcac
    16681 tttttataac atatattaaa tccaaaatat ctttagcact tgcttctcct tgattgacta
    16741 caaatccaca atgcttctca gatacttgtg ctcctcttaa agtaagacct ctcaatccag
    16801 tttcttctat taatttacct gcaaaatatc ctgttggtct cttaaatgta cttcctgcac
    16861 tcgcaaaatt taatggctgt ttagttattc ttcttgtcgc taaatcatcc attaaacttt
    16921 ttatttcatc ataatttcct tcttgtaatt ctagttctgc tgataaagct atatatccgt
    16981 ttttcgatat tatacttttt ctatacccaa attccatctg ttcatttgat aattcaaata
    17041 tattgccttc catatccatc agtcttactg acttcactat gtccttcatt tcaccgccat
    17101 atgctccagc attcatggct agtgctcctc ccaatgtgcc aggtattcct gatgcaaact
    17161 caaacccttt taattcttgc ttttggagag ctttacctaa aactgataat aaagctcctg
    17221 actgagcagt catcctagta tcatttatct cataagaatt gaaattatca gctaattcta
    17281 ttaccactcc tcttattcca ccatctttaa ttaaaatgtt tgaaccattt ccaattatta
    17341 aatacggaat agattcttct tttaccaact taagtacatt ttttaattgt tcttcagttc
    17401 taggtctaac taatatatct gctggtcctc ctaccctaaa tgaaatatgt tttttcatag
    17461 gttcatcgac ttttatatcc tctttactta atatatttaa taaatttata tatatatctt
    17521 gattattcat tgtatttcct cccaaattat actgaacttt agctgtcatt attatttaaa
    17581 ttacattagt atatagtatt aactatattc aataatatat gattttgctt atcatatgga
    17641 ttttatattt taagcttctt aattacaaac tatattcaat aaatatataa taaagtaaaa
    17701 aaaaaaaaat aacaacatat aaaagaatat aaataattta aaaaaagcat taatatcact
    17761 gatatcaatg ctttaaaagg caatttaatt cgtattattt atctaactat ttttgacata
    17821 gcaaagataa taaatccctc caactactat ggaacctcca acgatatttc caagcgttac
    17881 aaataccaaa ttagttataa attgaaccat agttatattt gctcctaatg ccattcccat
    17941 tggtataaaa aacatatttg caacactatg ttggaatccg cataatacaa ataacattat
    18001 tactgtccaa catgaagcca attttgacat tatatcttga gatgctgtag ccatccatac
    18061 agctaaaacg actaaaatat tacaaagtat tgcttttaaa aacattgatg ctatagttga
    18121 attaacctta gcttctgcca cagctatagc ttttgtagct gcatctcctg tcaatgtaga
    18181 agaataaaac acaagtgcaa ctagcagaat tgaacctact aaatttgcaa aataaaccag
    18241 tccccagttt cttaacattt ttcctaatgt tatttttttg tccataagtg caatagccat
    18301 taaggtgttt ccagtgaata attctgctcc tgccaccact acaagcataa gacctactgg
    18361 aaagaccata gcacttaaaa agctagctac ccctgggtct atcttgccaa aagtttgact
    18421 aactatcata ctccccaatg ccccaaaacc tataaatgct cctccaaata aacctaataa
    18481 gattgcattt tgtgtcgaaa tgtttgcttt tttaatgcct gcacttatag ttgcttgtgc
    18541 agtttcccca ggtgtcaaga atcctttact cataaatcct tcccccttaa aaattatact
    18601 ttcccttgtc tttttatggc aataattata taataacaat atgatatata ataaatttta
    18661 ttaacataaa aaatatacta tatacttata tat
//
LOCUS       DAEPXF010000070        18682 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain HB27
            SAMN13376389-rid12479423.denovo.074, whole genome shotgun sequence.
ACCESSION   DAEPXF010000070 DAEPXF010000000
VERSION     DAEPXF010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13376389
            Sequence Read Archive: SRR10518432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 18682)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18682)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001069624.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001069624.1
            Genome Coverage       :: 315x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/15/2021 01:54:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,165
            CDSs (total)                      :: 4,076
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 89
            rRNAs                             :: 10, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 10 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 19 of 69
            Pseudo Genes (incomplete)         :: 44 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 10 of 69
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18682
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13376389-rid12479423.denovo.074"
                     /strain="HB27"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="China: Hebei"
                     /collection_date="2017"
     gene            complement(147..539)
                     /locus_tag="KRL67_002062"
     CDS             complement(147..539)
                     /locus_tag="KRL67_002062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HBH1522939.1"
                     /translation="MAALKSFEKPLTPEEEIEYLTKFKIENDKSAKDTLIERNMRLVA
                     YIAKKYNNSTEDQDDLISIGTIGLIKAIETYNIDKGTRLATYASRCIENEILMNIRSN
                     KKNKTQVSLQDPIGTDKEGNEINTTFYM"
     gene            832..1041
                     /locus_tag="KRL67_002063"
     CDS             832..1041
                     /locus_tag="KRL67_002063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH1522940.1"
                     /translation="MKSIRVYKNMNIRRCNFTILKKVNAINYLYKVDYKQKISVIVNT
                     VKDEGGVLIYIYMGRNFDYNEKTYS"
     gene            1083..1445
                     /locus_tag="KRL67_002064"
     CDS             1083..1445
                     /locus_tag="KRL67_002064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH1522941.1"
                     /translation="MKIRKSGFISIECIISIAILYVVVYLVSTSLYNCYSFISRSMSD
                     REMLSTAKKYIEDEKYRIQNSKYELIEDKIEKNYINGYEINSRIKQILDYYQCYEINI
                     EIKNEFKELRFNSYVTRK"
     gene            1429..2145
                     /locus_tag="KRL67_002065"
     CDS             1429..2145
                     /locus_tag="KRL67_002065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH1522942.1"
                     /translation="MLLENKGSILIITLIIFSIISTVCIMCIGLIYTHNNMFELGYKD
                     IQLKEISLSGIEISVSNVLSCVEEAIAVSNNEDEFREYFLGNNMIECIKKIKDTSYSS
                     LEELSLKVENNTIYDRGDFFEFDIESNVREKEFLKEIKVRVKIKNPWVGIDIEDYMDK
                     SNNNKIDDKTFNNSKVSENNEILNQFKNESENISNQEGVEFTEEDEEYEEELKQNIIE
                     NIKKNFDEKKLVQVYKYRGV"
     gene            2165..2584
                     /locus_tag="KRL67_002066"
     CDS             2165..2584
                     /locus_tag="KRL67_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH1522943.1"
                     /translation="MTVIELIVSISIMLLIVTLSFPKDNLENHKINLFAHQLCSDIRY
                     VRRINMFSNYNTYIYYINQGKHEGYVLRKDGKNIKSIMLPKNAKIARSIEIIKFKSDG
                     SPQKASTIEIYNNRLRKTITITPVSGRVLLKEGKYET"
     gene            2574..3098
                     /locus_tag="KRL67_002067"
     CDS             2574..3098
                     /locus_tag="KRL67_002067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH1522944.1"
                     /translation="MKHKYGYLLLESVVSLSSMVIIMLVLYSIFLSTINLKLKVEDKI
                     ELQQQSLEIIKSMEGIISNSMGIMNVSNYEETFKKTTSIKCRYVDENNNEENISNKEI
                     ILNERRNKLFVNSLNGESSQAGGYEIGDYVDEMYVSITNNGQYVNIKLKLSKRSQKYE
                     TDFKIKVWNFSESV"
     gene            3410..3967
                     /gene="efp"
                     /locus_tag="KRL67_002068"
     CDS             3410..3967
                     /gene="efp"
                     /locus_tag="KRL67_002068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HBH1522945.1"
                     /translation="MVSAGDFRKGVTFEKDGQPCLVVDFQHVKPGKGAAFVRTKYKNL
                     KTGAIREESFNPSEKFPKAVIDTRQMQYLYNDGELYYFMDQENFEQIPLNYEQVEDAI
                     KFLKENEVATIRFYQGQPFQVEAPNFAELEVTDTEPGIKGDTASNVTKAATVETGAVV
                     QVPLFINTGDKVKIDTRTGEYLSRV"
     gene            4059..4514
                     /locus_tag="KRL67_002069"
     CDS             4059..4514
                     /locus_tag="KRL67_002069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HBH1522946.1"
                     /translation="MKHLYEEVAYLKGLAEGLEISAESKEGKMIHKIVDALEVFADAI
                     VTLDEEQEELQDFVESIDEDLADLEEDIADMEEDLYEEDDDEDDEDFSYIEMECPNCG
                     ELVEIDEDLLYDDEVDVVCPDCKAVILSSEDDCDDDCTCGGCSSCDDRE"
     gene            4677..5387
                     /locus_tag="KRL67_002070"
     CDS             4677..5387
                     /locus_tag="KRL67_002070"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HBH1522947.1"
                     /translation="MKISKKLLDNIQRFENVINYKFKNKEYILEALTHSSYSNENKKY
                     NFNERLEFLGDSVLGIVISDYLFNEEANLPEGELTKLRANIVCEDSLSEVANDINLGI
                     HMLLGRGEEATGGRHRTSILADAFEAVIAAIYLDGGFESARQFILHHMENIIYDSRKG
                     NIFRDYKTHLQEVLQGNGENNIWYRLIEEKGPDHNKRFVMEVGINDDVLGIGEGKSKK
                     EAEQLAAKIALKKKLWEK"
     gene            5441..6511
                     /locus_tag="KRL67_002071"
     CDS             5441..6511
                     /locus_tag="KRL67_002071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HBH1522948.1"
                     /translation="MKKRIIPIFVPHKGCPHDCIFCNQKKITGVSTDVTSEDARNIIE
                     ECLETIDKDADVEIAFFGGSFTAIDIDIQKSLLSVAKEYVEKGLVKDIRMSTRPDCID
                     EDILSMLKEYKTSIIELGVQSLDEKVLLDSIRGHQSEIVYKSSKMIKNSGIKLGLQMM
                     IGLPADTEEKCIFTAKKFVELKPDCVRVYPTLVVKDTGLEKLMEQNKYNPFTLEESVN
                     IVKKVLVLFYVNNINVIRVGLQATDDIQIGKAVLAGPYHPAFRELVEADIIKDYLEFV
                     ILQNKNIKQMLVKANKKNISKIIGNKKTNVKYMEEKFGVLLKTQESDLDINQLEIVLD
                     GKSLISANMRDIHRKLYDIYDL"
     gene            6539..10093
                     /gene="smc"
                     /locus_tag="KRL67_002072"
     CDS             6539..10093
                     /gene="smc"
                     /locus_tag="KRL67_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HBH1522949.1"
                     /translation="MYLKRLELKGFKSFPVKTDIIFKEGITAIVGPNGSGKSNISDAV
                     RWVLGEQSIKSLRGDKLEDVIFAGTDTKKPMNYCEVALTIDNSENQLELDFTEVTIRR
                     RAYRNGESEFFLNNKSCRLKDIKEVFLDTGIGKDGYSIIEQGKVDEILSNNPLSRRKV
                     FDEACGISKYRYKKQEAERNLSNTKENLERIDDVYIEIENQLKPLFNQQTKAKKYLEI
                     SEKLKILEVNSFIREIEGIEKELSEVNEHRKVIEKELNEKEEQKNVVEKKQEDINKEV
                     EVLQDVIEKSVDYINSIKGVISKKESQINLIKERIRNFTNEISRKNLEINDIKEKLNE
                     NKQYIKELESNKLSGSEELSTLQKNIKVLEASKDKQKIKLESLNNEIELLKESIIDIL
                     NKKQEFSNKLSTLNANKENMNIRDENINSEITELNKNIEIKSSELDTINKEFNMQNEN
                     LKNVNNRHKELSTNLQDSISEHNKLEDEIQKSKYNLNGYNSKLNVYIDMENHYEGFNR
                     GVKEVLKNKNLKGVHGALGQIINVPEKYEKSIEAALGAYMQNIITDNEFSAKSAINYL
                     KQNNLGRVTFLPLNIIKSNKISLGNLKANTKFIGIASDLITFDEKYRNIIENILGRTI
                     LINNIDEGIKFAKETGHRFKIVTLDGEILNPGGSLTGGSLKTNGNILSRKRYINEYTE
                     KINNIKNEISHLELKRESLDKNVKNIKNEIDSHESKIKDLEKSIIIKSTSIKNVESEI
                     ESLKGSITKLENEKNDLNSNLNYTLEKSNDVRKNIEELDGLYNKNKEKIDALNEEIKR
                     YNDLYDKEKSEFDELNLSLVKKTEVYNSIVRDIKRISGENCELEEKNKQLEESLNYEE
                     HEIIKLQDSIATEEKEKENLTKQLGDSNRNLETRKIAKDDLKNSFDEINKELKTIDRQ
                     HIELKESLFKVGGRLERLKTSQDTYINKLFEQYDMTLVQALEIKDEDLDIDRKFLESL
                     KREIRSLGNINIDSIKEYEEIKERYDFYSEQKQDLEESMEEIEKLIHTLEENMKSEFE
                     IKFEEISKNFKYVYKRLFGGGCGELTILDKENLLESDILITAQPPGKKMKNLNLLSGG
                     EKALTAISILFAILITKPTPFCILDEIEAPLDDANIFRFGEFLKDLSKETQFISVTHR
                     RGTMEAADYIYGVTMQEKAISKVISLKLKEAQEITDII"
     gene            10120..11400
                     /gene="ftsY"
                     /locus_tag="KRL67_002073"
     CDS             10120..11400
                     /gene="ftsY"
                     /locus_tag="KRL67_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HBH1522950.1"
                     /translation="MLKKLFGFGKDKEKEIEKKDAEEEIEVEDSVDNLENLEETIFSG
                     LEEEVIDKVEDIEEKTEESVNNEDTEEIENFEKIEVGMDINGKKVENISNNDSQEDII
                     EEVENYDEIESKEIEDKEEKKVNLFERLKQGLTKAKQGITDRIDEVLKSYTKIDEELL
                     EDLEEILITADVGVNTTMDIIERLRDKIKQKGITEPIKVREELKSIVEDILTNENSTL
                     DIEPAPCIILMVGVNGVGKTTTIGKLANRYKKDGKKVLLAAADTFRAAATEQLEIWAN
                     RTNVDIIKHQEGADPGAVVFDAIKAAKARKTDVLICDTAGRLHNKANLMNELGKVFKI
                     VDREFPEAKREVLLVVDATTGQNAVVQAKTFKEVADITGIVLTKLDGTAKGGVVLAVK
                     SEVDVPVKLIGVGESVEDLQDFNAKSFSDALFGN"
     gene            11503..11871
                     /locus_tag="KRL67_002074"
     CDS             11503..11871
                     /locus_tag="KRL67_002074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding protein"
                     /protein_id="HBH1522951.1"
                     /translation="MNIEKMVEIGLLFEQYKELLTDKQKEIVALYYEEDYSLGEISEN
                     LNVSRQGVYDTLKRSEKILRDYEEKLHLVSKIQEQEKNIKIIKDKIIDIKEDLLHNRD
                     CANLIPKLENIEDVCREMIK"
     gene            11868..13220
                     /gene="ffh"
                     /locus_tag="KRL67_002075"
     CDS             11868..13220
                     /gene="ffh"
                     /locus_tag="KRL67_002075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HBH1522952.1"
                     /translation="MIFEGLSDKLQGAFSKLKSKGKLTEVDVKNAMREVKLALLEADV
                     NFKVVKDFIKKVQERCVGQEVMQSLTPAQHVIKIVNEELTSLMGDVQSKVMISSKPPT
                     ILMMVGLQGAGKTTTAGKLGGYFKKQGKKPLLVACDIYRPAAIKQLQVVGEKLDIPVF
                     NMGDKENPVNIAKAGLSHAIKNANDLVIIDTAGRLHIDEVLMDELKSIKSEVKPHEIL
                     LVVDSMTGQDAVNVAESFNEALGVDGVVLTKLDGDTRGGAALSIRAVTQKPIKFMGMG
                     EKLDDIEPFHPDRMASRILGMGDVLSLIEKAQENIDLEKAKELEQKIKKQELDFEDFL
                     QQMEQIQNMGPLDKILGMIPGMGNVKDQLGDVDLNGKEMKRTKAIIQSMTTYERRNPG
                     VLNASRKKRIAKGSGTSVQDVNRLIKQLNEMKKMMKMFAGSQKSMKKRGGLGGLPFFK
                     "
     gene            13258..13530
                     /gene="rpsP"
                     /locus_tag="KRL67_002076"
     CDS             13258..13530
                     /gene="rpsP"
                     /locus_tag="KRL67_002076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000268750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HBH1522953.1"
                     /translation="MAVKIRLKRMGANKKPFYRIVVADSRAPRDGKFIEEIGYYNPIS
                     EPKQVRINDEKAIKWLATGAQPTEVVKKLLVKNGVIEKFEASKQAK"
     gene            13595..13822
                     /locus_tag="KRL67_002077"
     CDS             13595..13822
                     /locus_tag="KRL67_002077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HBH1522954.1"
                     /translation="MKELVVDIAKALVDNPDSVVVEEFEDNDGIVLKLTVAQEDMGKV
                     IGKQGRIAKAIRTVVRSVANRENIKVSLEIV"
     gene            13930..14445
                     /gene="rimM"
                     /locus_tag="KRL67_002078"
     CDS             13930..14445
                     /gene="rimM"
                     /locus_tag="KRL67_002078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA processing protein RimM"
                     /protein_id="HBH1522955.1"
                     /translation="MKEKLTHFKVGQIVNTQGLKGEVRVYPLTDDIERFDELKDFYLG
                     KDLNDKLAVEKVRYKGSMVIMKIKGIDSIEKAEKLKNKFMYVSREDSRELEEGEFFIA
                     DMIGMEVLTVDGKYVGILEDVLQYSANDVYVVKGEEDKEFMIPAIKKFVPTIDIDERK
                     MIIDPIKGMID"
     gene            14454..15149
                     /gene="trmD"
                     /locus_tag="KRL67_002079"
     CDS             14454..15149
                     /gene="trmD"
                     /locus_tag="KRL67_002079"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000686892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HBH1522956.1"
                     /translation="MRFHIMTLFPEIFNSYMDESIMKRAVEKGIIEVHIYNIRDFSNN
                     KHKKVDDYPFGGGAGMVMTPQPVYDTYKHIITTHNINSPSVIYLTPKGKVYNQSIAKQ
                     LSLKEDIILLCGHYEGIDERIIDLIVTDEISIGDYVLTGGELPALIMIDSISRLIPGV
                     LNQEESFEEESFKDNLLEYPHYTRPRDFEGLKVPEVLLSGNHKKIDEWRREESIRITK
                     ERRFDLYKKSNEK"
     gene            15334..15684
                     /gene="rplS"
                     /locus_tag="KRL67_002080"
     CDS             15334..15684
                     /gene="rplS"
                     /locus_tag="KRL67_002080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HBH1522957.1"
                     /translation="MNEMLRAIEQEQLKNEVPNFGPGDTVKVHVRIIEGKRERIQVFE
                     GVVLKRQGGGARETFTVRKMSFNVGVERTFPVHSPKIEKIEVTRKGKVRRAKLNYLRG
                     RVGKAAKIKEARNK"
     gene            16005..16901
                     /gene="ylqF"
                     /locus_tag="KRL67_002081"
     CDS             16005..16901
                     /gene="ylqF"
                     /locus_tag="KRL67_002081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HBH1522958.1"
                     /translation="MSNEYEEYLMDDNLHINWYPGHMKKTKELVKNNLKLIDVVIELL
                     DARIPFSSKNPDIDRLVGDKPRVVVLNKSDMADRDKLNQWIEYYKKINIKAIPVDTIK
                     GVGINKIIEECKNVTREKMSSLKDKGRKERAIRIMIVGVPNVGKSSLINKLTGRKSTQ
                     TGDKPGVTKGKQWVRLKGNLELLDTPGILWPKFEDQEVALNLAFSRAIKDEILDTETL
                     ALRLIEKLMKIEPEKLKARYKLDCLGETPIETMDMIGHKRGFITGKKELDYTRIATTV
                     LNEFRDGKIGNITLEVPENVKR"
     gene            17290..18582
                     /gene="brnQ"
                     /locus_tag="KRL67_002082"
     CDS             17290..18582
                     /gene="brnQ"
                     /locus_tag="KRL67_002082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HBH1522959.1"
                     /translation="MGKTKDVIVFGFALFAMFFGAGNLIFPPYLGIITGPEWLIAFLG
                     FTFADAGLALLAVMATAKFDGNVVEMFKRCGMKLGILIGCADILCIGPFLAIPRTGAT
                     TYEMGIMPLFGTSIPVLLFCILFFAISYVLTIRPSKVVDIVGQFLTPALLIALAFIII
                     KGIISPLGDIVDKPMIPNVFAEGIGQGYQTMDAFAAIALASVLIVSLNDKGYSTISDK
                     LKMIGKAGVLACGGLALVYGGLCFLGATVSTMYGTDAVQSQVIVNITEGLLGNVGKVI
                     LAVVVSLACLTTSIGLTSATGQYFSRLTKGKLSYEKIVLAVSVFSAVVASFGVGTIIK
                     IASPILSIVYPPSIVLIILAFFNEKIKNDNVYKGAVYMSLLVSILTVISSYGVAVPVV
                     NSLPLSSLGFNWVVPVIIAGIIGNFIPSKSQSNTLGTN"
BASE COUNT         7879 a         1747 c         3467 g         5589 t
ORIGIN      
        1 gtatgtagag gtatttttaa aaagttaaaa tatgtactta ctgactaatg ataatcttat
       61 atgtatttta gtttgagtga tttaagaaaa gtgttttgaa gggggattat tttataaagt
      121 aaataaatat atataatagt agaaaattac atataaaaag tagtgtttat ttcattacct
      181 tctttatctg ttccgatagg gtcttgtaga gatacttgag ttttattttt tttgttagat
      241 ctaatattca ttaaaatctc attttcaata catctagaag catatgttgc taatcgagtt
      301 cctttatcta tgttataagt ttctatagct ttaattaatc ctattgtacc tatggatatt
      361 aaatcatcct ggtcttccgt tgaattatta tatttctttg ctatataagc tactaatctc
      421 atgtttcttt caattaatgt atcttttgca cttttatcgt tttctatttt aaatttagtt
      481 aaatactcaa tctcctcttc aggggttaag ggtttttcaa aagatttaag agctgccaat
      541 aggtcacccc cttttcaacg gtcactttaa attatatgaa ggagacttat ccaagttgac
      601 tgttatctaa taaaataata tgcatatttt tagaatttaa aacatatatt tgcaaacttt
      661 tgaatggaat atgacataaa gagtgggaat ttaaatttaa gtttttgttg tatatatatg
      721 aaacaaggcg aagaaattat tgatttataa agtataaatt ggaattgagg tgtcaataaa
      781 tataaaagga aatagtattt atatatgttt tgatgttgag gtaaaagatt catgaaaagc
      841 attagagtct ataaaaatat gaatattagg aggtgtaatt ttacaatact taaaaaagta
      901 aatgcaatta attatttgta taaagttgat tacaagcaaa aaatatcagt tatagttaat
      961 actgttaagg atgaaggtgg tgtgttaatt tatatatata tgggaagaaa ttttgattac
     1021 aatgaaaaaa cttatagtta aatgtttata tataaaatat tatttagggg ttgtttttat
     1081 tattgaaaat aagaaagagt ggttttatat caatcgaatg tataataagt attgctatat
     1141 tatatgtggt tgtttattta gtttcaacat cattgtataa ttgttatagt tttatcagta
     1201 gaagtatgtc tgacagagaa atgttaagta cagcaaaaaa atatatagaa gatgagaagt
     1261 atagaataca aaatagtaag tatgagttaa ttgaagataa gatagaaaaa aattacataa
     1321 atggatatga aattaacagt agaataaagc aaattttaga ttattatcaa tgctatgaaa
     1381 taaatataga gataaaaaat gaatttaaag aactgaggtt taatagctat gttactagaa
     1441 aataaaggct ctatattaat aataacattg ataatatttt ctattataag tactgtgtgc
     1501 attatgtgta taggtttaat ttatacacat aataatatgt ttgagttagg atacaaggac
     1561 attcaactaa aagaaatttc tttaagtgga atagaaatat cagtaagtaa tgtactttct
     1621 tgtgttgagg aagctattgc tgtatctaat aatgaagacg aatttagaga atatttctta
     1681 ggaaataaca tgattgaatg tataaaaaaa ataaaagata catcttatag tagtttagaa
     1741 gaactttctt taaaagtaga aaataatact atttatgata gaggagattt ttttgaattt
     1801 gatatagaaa gtaatgttag agaaaaagaa tttttaaaag aaataaaagt aagagtgaaa
     1861 attaaaaatc catgggtagg aattgacatt gaagattata tggataaatc gaataacaat
     1921 aaaattgatg acaaaacatt caataattct aaagtgtctg aaaataatga aattctaaat
     1981 caatttaaaa atgaaagtga aaatataagt aatcaagagg gtgttgaatt tacagaagaa
     2041 gatgaagaat atgaagaaga acttaaacaa aatataattg aaaatataaa gaagaatttt
     2101 gatgaaaaaa aactagtaca ggtatataag tataggggtg tataactttg aaaaaagaag
     2161 gaatgtgact gtaatagaat taatagtatc tatcagtata atgctcttga tagttacatt
     2221 atcttttcca aaagataatc ttgaaaatca taaaataaat ttatttgcac atcaactttg
     2281 ttcagatata aggtatgtta ggcgaattaa tatgtttagc aactacaata catatatata
     2341 ttatataaat caaggtaaac acgaaggtta tgtattgaga aaagatggaa aaaacattaa
     2401 atcaataatg ctacctaaaa atgcaaaaat agctagaagt attgaaataa taaagtttaa
     2461 atctgatggg tcgcctcaaa aggcgtctac aatagagata tataataata gattaagaaa
     2521 aactataaca ataacacctg taagtggaag agtacttctg aaagagggaa aatatgaaac
     2581 ataaatatgg ttacttgctt ttggaaagtg tagtatcatt gtcttcaatg gtgataatta
     2641 tgctagtttt atattctata tttttgtcta caataaactt aaagttaaaa gtagaagata
     2701 agatagaact acaacaacaa agtcttgaaa tcataaaatc tatggaagga attattagca
     2761 attctatggg aataatgaat gtatctaatt atgaagaaac atttaaaaag acaacatcta
     2821 taaaatgtag atatgtagat gaaaataata atgaagaaaa cataagtaat aaagaaatta
     2881 tattgaatga aagacgaaat aagttatttg taaattcttt aaatggcgaa agtagtcaag
     2941 caggtggcta tgaaattgga gattatgtag atgagatgta tgtatcgatt actaataatg
     3001 gtcaatatgt taatattaaa ttgaaattat caaaacgtag tcaaaaatat gagactgatt
     3061 tcaaaataaa ggtatggaat tttagtgaaa gtgtataaaa atacataaat ttaagagaaa
     3121 gaaatgacat caataagtca tataaaatat aattattgag aactatataa ctaaataata
     3181 tcaatatgta aagtgtatag attaagaata tagaggataa taataaaaat atagaataag
     3241 taatttgcca aatacttgtt tatattgtat aacttatgat attatgatat tagtaaaaat
     3301 ttgtaatcct gacttgacag aattcaagta agagatataa ttttaaaggc atagtaatgt
     3361 aaaatttact aaaaaataag aaatttattt taatatggag gaacaaaaaa tggtatcagc
     3421 aggtgatttt agaaaaggtg ttacatttga aaaagatgga caaccatgtt tagtagttga
     3481 ttttcaacac gttaagccag gtaaaggagc tgctttcgtt agaactaaat acaaaaactt
     3541 aaaaacagga gctataagag aagaaagttt caatccaagt gaaaaattcc ctaaggctgt
     3601 tatagataca agacaaatgc aatatctata caatgatggt gagttatatt attttatgga
     3661 tcaagaaaat tttgaacaaa taccattaaa ctatgagcaa gttgaagatg caattaagtt
     3721 cttaaaagaa aatgaagttg ctacaataag attttaccaa ggacaaccat tccaagtgga
     3781 agctccaaac tttgcagagc tagaagttac agatacagag ccaggtataa aaggtgatac
     3841 agcaagtaat gtaactaaag cagctacagt tgaaactgga gcagttgttc aagttccatt
     3901 atttataaat actggagata aagtaaagat agatactaga acaggtgaat atttatcaag
     3961 agtataaaaa tttatgtata taaaagctta tatgtcttaa taatataaga tatatgagct
     4021 tttagtttat atataaaaaa gaaagcgagg ggtgttacat gaaacattta tatgaagaag
     4081 ttgcatactt aaaaggccta gcagaaggac tagaaataag tgctgaaagt aaagaaggga
     4141 aaatgataca taaaatagtt gatgcattag aagtatttgc agatgctatt gtaacattgg
     4201 atgaagaaca agaagaactt caagattttg ttgaatcgat agatgaagat ttagcagatt
     4261 tagaagaaga tattgctgac atggaagaag atctttatga agaagatgac gatgaggatg
     4321 atgaggactt cagctatata gaaatggaat gcccaaattg tggagagtta gtagaaatag
     4381 acgaagattt gttatatgat gatgaagtag atgttgtttg tcctgattgt aaggctgtga
     4441 tattatcatc agaagatgat tgtgatgatg attgtacatg tggtggttgt tcaagttgtg
     4501 atgatagaga gtaaaaattc ataagaatta aaactgccta tgatgcctat gagatatttt
     4561 gcttcatagg tagttttatt tttatgagga agggtattat gtattttggt tttgcaatac
     4621 ttggacaaaa atagtacact agaatttata gaataggatt ttggagggat gtaaatatga
     4681 aaataagtaa aaaattacta gataatatac aaagatttga aaatgttata aattataaat
     4741 ttaaaaataa agaatatata ctagaagctc ttactcatag ttcatattct aatgaaaata
     4801 aaaaatataa ttttaacgaa aggttggaat ttttagggga ttcggtttta ggcatagtga
     4861 taagtgatta tttatttaat gaagaagcta atctgcccga aggggaacta actaaactta
     4921 gagcaaatat agtttgtgaa gattcgttaa gtgaagtagc taatgatata aatttaggta
     4981 ttcacatgtt attaggtaga ggagaagaag caacaggagg aagacatagg acttcaatat
     5041 tagcagacgc atttgaagct gtaatagctg ctatatatct tgatggaggt tttgaaagtg
     5101 caagacagtt tatacttcat catatggaaa atataattta tgattcaaga aaaggaaata
     5161 tatttagaga ctacaaaacg catcttcaag aagtgttaca agggaatgga gaaaataata
     5221 tctggtacag gcttatagaa gaaaaaggac cagaccataa taaaagattt gtaatggaag
     5281 ttggaattaa tgacgatgta cttggtatag gagaaggcaa gagtaaaaaa gaagctgaac
     5341 agttagctgc taaaatagct ttaaagaaaa aattatggga aaaataaata taatatgtaa
     5401 aaatataaaa agaaatattt atattagaag gatgttgtag atgaaaaaga gaattatacc
     5461 tatatttgtt cctcacaaag gatgtcctca tgattgcata ttttgtaatc aaaaaaaaat
     5521 aacaggtgtg agtactgatg ttactagtga agatgcaaga aatataattg aggaatgttt
     5581 agagactata gataaagatg cagatgtaga aatagcattt tttggcggta gctttactgc
     5641 tatagatatt gatatacaaa aaagtttatt atcagttgct aaagaatatg tagaaaaagg
     5701 tcttgtaaaa gatattagaa tgtctacaag accagattgt attgatgaag atatattaag
     5761 tatgctaaaa gaatataaga caagtataat tgaactagga gtacaatcct tagatgaaaa
     5821 agtattatta gatagtataa gaggtcatca atctgaaatt gtatacaaga gttcaaagat
     5881 gataaaaaat agtggaatta aacttggatt acagatgatg attggtcttc cagctgacac
     5941 agaagaaaag tgtatattta cagcaaaaaa atttgtagag ctaaaaccag attgtgttag
     6001 agtctatcca actttggtag taaaagatac aggtcttgaa aagttaatgg aacaaaataa
     6061 atataatcca tttactcttg aagagagtgt aaatatagta aaaaaagttc ttgtattgtt
     6121 ttatgttaat aatatcaatg taataagagt tgggcttcaa gctactgatg atatacaaat
     6181 tggaaaagct gttttagcag ggccatatca tcctgcattt agagagttgg tagaagcaga
     6241 tataataaaa gattatttag aatttgttat tttgcaaaat aaaaatataa agcaaatgtt
     6301 ggtcaaagct aataaaaaga atatttctaa aatcatagga aataaaaaaa caaatgttaa
     6361 atatatggaa gaaaaatttg gtgtgctttt aaaaacacaa gaatcagatt tagatataaa
     6421 tcaattagaa attgtgttag atgggaaaag tttaattagt gctaatatga gagacattca
     6481 tagaaaattg tatgacattt atgaccttta gttttaataa tgaagggaga gaaaagattt
     6541 gtatttaaaa agactagagt taaagggatt taaatccttt ccagtaaaaa cagatattat
     6601 ttttaaagaa ggaataacag ccatagtagg accaaatggg agtggaaaaa gtaatatatc
     6661 tgatgctgta agatgggtat taggagaaca gagtattaag agtcttagag gcgataaact
     6721 cgaagatgta atatttgcag gtacagatac aaaaaaacct atgaattatt gtgaagtggc
     6781 ccttactatt gataattctg aaaatcaatt ggaattagat tttacagaag taactataag
     6841 aagaagggct tatagaaatg gtgaaagtga attttttcta aataataaaa gctgtagatt
     6901 aaaagatata aaagaagtct ttttagatac tggtattgga aaagatggtt attcaatcat
     6961 agaacaagga aaagtagatg aaatattaag taataatcct ttaagcagaa gaaaagtttt
     7021 tgatgaagct tgtggcatat caaaatatag atataaaaaa caggaagcag aaaggaattt
     7081 aagtaataca aaggaaaatt tagaaagaat agatgatgta tatatagaaa ttgaaaatca
     7141 attaaagcct ctttttaatc aacagacaaa ggcaaaaaag tacttggaaa taagtgaaaa
     7201 attaaagata cttgaagtaa atagctttat aagagaaatt gaaggaatag aaaaagaatt
     7261 aagtgaggtc aatgaacatc gtaaggttat tgaaaaggag ctcaatgaaa aagaagaaca
     7321 aaaaaatgtt gtagagaaaa agcaagaaga tattaataaa gaggtagagg tattacaaga
     7381 tgtaattgaa aaatctgtag attatataaa ttcaattaaa ggagttatat caaaaaaaga
     7441 gtcccaaata aatttaatta aggaaagaat aagaaatttt acgaatgaaa ttagtagaaa
     7501 aaatttagaa ataaatgata taaaagagaa gctaaatgaa aataagcaat acataaaaga
     7561 attagaaagt aataaattat caggtagtga agaactatct accttgcaga aaaatataaa
     7621 agtattggaa gcaagtaaag ataaacaaaa aattaagtta gaatccctaa ataatgagat
     7681 agaattattg aaagaaagta taattgatat attaaataag aaacaagagt ttagtaataa
     7741 gctgtccact ttaaacgcaa ataaagaaaa tatgaatatc agagatgaaa atataaattc
     7801 agagataaca gagttaaaca aaaatattga aataaaatca tctgaattag atactataaa
     7861 taaagaattt aatatgcaaa atgaaaattt aaagaatgta aataatagac ataaagaatt
     7921 atcaactaat ttgcaagatt caattagtga acacaataaa ctagaagatg aaatacagaa
     7981 aagtaaatat aatttaaatg gatataattc aaaattaaat gtctatatag atatggaaaa
     8041 tcattatgaa gggtttaata gaggagtaaa agaagtacta aaaaataaaa atttaaaagg
     8101 ggttcatgga gcattaggac agattataaa tgtacctgag aaatatgaaa aatctataga
     8161 agcagctctt ggtgcttata tgcaaaatat aataactgat aatgagttta gtgctaagtc
     8221 tgcaataaac tatctaaagc agaataattt aggtagagta acatttcttc cattaaatat
     8281 aattaaatca aataaaataa gtctaggaaa tttaaaagca aatactaagt tcataggaat
     8341 agcaagtgat ttaattacat ttgatgaaaa gtatagaaat ataattgaaa atatacttgg
     8401 aagaacaatt cttataaata atatagatga gggcataaaa tttgcaaaag aaactggtca
     8461 taggtttaaa atagtaactt tagatggtga aatcttaaat ccaggaggat ctttgactgg
     8521 gggaagtcta aaaactaatg ggaatatact atcgagaaaa agatacatta atgaatatac
     8581 agaaaagata aataatatca agaatgaaat ttcacaccta gaattaaaaa gagaatcttt
     8641 agacaaaaat gtgaaaaata tcaagaatga aattgattca catgaaagta aaataaaaga
     8701 tttagaaaaa agtataataa taaaaagtac aagtataaag aatgttgaat ctgaaattga
     8761 atcgttaaaa ggtagtatta ctaaattaga aaatgagaag aatgatttaa attctaacct
     8821 aaattataca cttgaaaaaa gcaatgatgt tagaaaaaat atagaagagt tagatggttt
     8881 atataataaa aataaagaaa aaattgatgc attgaatgag gaaataaaaa gatacaatga
     8941 tttatatgat aaagaaaaat ctgaatttga tgaattgaac ttaagtttag tgaagaaaac
     9001 agaggtttac aatagtatag ttagagatat aaaaagaata tctggtgaaa attgtgagct
     9061 agaagaaaag aataaacaat tagaagagtc attaaattat gaagaacatg aaataattaa
     9121 attacaagat tctatagcca ctgaagaaaa agaaaaagaa aatttaacaa aacaactagg
     9181 ggatagtaat agaaatcttg aaactagaaa aatagcaaaa gatgatttaa aaaatagttt
     9241 tgatgaaatt aataaagaat taaaaactat agatagacag catatagagc ttaaagaaag
     9301 tttatttaaa gttggtggaa gattagaaag attaaaaact agtcaagaca catatataaa
     9361 taagttgttt gagcaatatg atatgacttt agtacaagca ttggaaatta aagatgaaga
     9421 tttggatata gatagaaaat tcttagaaag tctaaagaga gaaataagaa gtcttggaaa
     9481 tataaacata gactctataa aggaatatga ggaaataaaa gaaagatacg atttttatag
     9541 cgaacaaaag caggatttag aagagtctat ggaagagata gaaaaactta tacatacttt
     9601 agaagaaaat atgaaatctg aatttgaaat taagtttgaa gaaataagta aaaattttaa
     9661 gtatgtatat aagagattat ttggtggagg ttgtggagaa ttaactattt tagataaaga
     9721 aaatttatta gaaagtgata tactaataac agctcaacca ccaggtaaaa aaatgaaaaa
     9781 tttaaaccta ttatctggtg gagagaaagc tttaacggca ataagcatat tatttgctat
     9841 attgattaca aagccaacac cattttgtat attagatgag attgaagcgc cacttgatga
     9901 tgccaatata tttagatttg gagaattttt gaaggattta tctaaagaga ctcaatttat
     9961 atcagtaact cataggagag gaactatgga agctgctgat tatatctacg gagttacaat
    10021 gcaagaaaaa gccatttcaa aagttattag tttaaaatta aaagaagctc aggaaataac
    10081 agatattata taacttagat tgacatagga ggaataacag tgttaaaaaa attatttggt
    10141 tttggaaaag acaaggaaaa ggaaatagaa aaaaaagatg ctgaagaaga aatagaagtt
    10201 gaagacagtg ttgataattt agaaaacttg gaagaaacca ttttttcagg acttgaagaa
    10261 gaagtgatag ataaagtgga agatattgaa gaaaaaactg aggaaagtgt caataatgaa
    10321 gacactgaag aaatcgaaaa ctttgaaaaa atagaagttg ggatggatat taatggaaaa
    10381 aaagtagaaa atatttcaaa taatgattct caagaagata taatcgaaga agtagaaaat
    10441 tatgatgaaa ttgagtctaa agagatagaa gataaagaag aaaagaaagt taacttgttt
    10501 gaaagactaa aacaaggatt gacaaaagca aagcaaggaa taactgatag aatagatgaa
    10561 gttttaaaat catatacaaa aatagacgaa gaattacttg aagatttaga ggaaatttta
    10621 ataacagcag atgttggtgt aaatacaact atggatataa ttgaaagact aagagataaa
    10681 ataaagcaaa aaggtattac tgaaccaata aaggtaagag aagaacttaa atctatagtt
    10741 gaagatattc ttacaaatga aaactctaca ctagatatag aaccagcacc atgtataata
    10801 ttaatggtgg gtgtaaatgg ggtcggtaag actactacaa taggtaaact agcaaataga
    10861 tacaaaaaag atggcaaaaa agttttatta gcagcagcag atacatttag agcagcagca
    10921 acagagcaat tagagatttg ggcaaataga accaatgtag acataataaa acatcaagaa
    10981 ggagcagacc ctggagctgt agtatttgat gctataaaag ctgcaaaagc tagaaaaaca
    11041 gatgtattaa tatgtgatac agcaggaaga ttacacaata aggctaatct tatgaatgaa
    11101 cttggaaaag tatttaaaat agtagataga gaatttccag aagcaaagcg tgaagtactt
    11161 cttgtggtgg atgctacaac aggtcaaaat gcagttgttc aagcaaagac atttaaagaa
    11221 gttgcagata taacaggtat agtacttaca aaacttgatg gaactgcaaa aggtggagta
    11281 gtccttgcag taaaatcaga agtagatgta cctgtaaaac ttattggagt tggagaaagt
    11341 gtagaagacc tacaagattt caatgcaaaa tcattttcag atgcattatt tggtaactag
    11401 tgttctttta aaaataatgg ttgacaagag tgttgttatg atataaaata caatagtgta
    11461 aagtaaataa cctttacagt atactgctag gaagtgtaag atatgaatat agaaaaaatg
    11521 gtcgaaattg gattgttatt tgaacagtat aaagaattat taactgacaa gcagaaagaa
    11581 atagttgctc tatactatga agaagattat tctttaggtg aaattagtga aaatctgaat
    11641 gtatcaagac agggtgttta tgatacctta aagcgttcag aaaaaatact aagagattat
    11701 gaagagaagt tacacttagt atcaaagatt caagagcagg aaaaaaatat taagattata
    11761 aaagataaaa ttattgacat taaagaagat ttattgcaca atagagattg tgctaattta
    11821 atccctaagc tagaaaatat agaagatgta tgtagggaga tgataaaatg atatttgaag
    11881 gattgtctga taaactacaa ggggcattta gtaagttaaa atctaaagga aaacttacag
    11941 aagtagatgt aaaaaatgct atgagagaag ttaaacttgc tcttcttgag gcggatgtta
    12001 actttaaagt agttaaagat ttcataaaaa aagttcaaga gagatgtgtt ggacaagaag
    12061 ttatgcaaag cttaactcca gctcagcatg taatcaaaat tgtaaatgaa gagcttacaa
    12121 gtttaatggg agatgtacaa agtaaagtaa tgatttcctc aaagcctcct acaattttga
    12181 tgatggttgg attgcaaggt gcaggtaaga ctactacagc aggaaaactt ggaggatatt
    12241 ttaaaaagca aggtaaaaaa cctctattag tagcttgtga tatttataga ccggcagcta
    12301 taaagcaact tcaagttgtt ggagaaaaat tggatatacc tgtattcaat atgggagata
    12361 aagaaaatcc agtaaatatt gcgaaagcag gcttaagcca tgcgattaaa aatgctaatg
    12421 atttagttat tatagataca gctggtagac ttcatataga tgaagtactt atggatgagt
    12481 taaagtctat taaatcagaa gttaagccac atgagatact tttggttgta gattctatga
    12541 caggtcaaga tgctgtaaat gttgcagaaa gcttcaatga agcacttggt gttgatggag
    12601 tagtattaac taaattggat ggagatacta gaggtggtgc tgcactttca ataagagcag
    12661 ttacacaaaa gcctataaaa tttatgggta tgggagaaaa gctagatgat atagaacctt
    12721 tccacccaga tagaatggca tcgagaatat taggtatggg agatgtctta agtttaatag
    12781 aaaaagcaca agaaaacata gacttagaga aagctaaaga attagagcaa aaaattaaaa
    12841 aacaagagct tgattttgaa gatttcttac aacaaatgga acaaatacaa aatatgggac
    12901 ctcttgacaa gatacttggt atgatacctg gtatgggaaa tgtaaaggac caacttggag
    12961 atgtagattt gaatggcaaa gaaatgaaga gaacaaaagc cataattcag tcaatgacta
    13021 catatgagag aagaaaccca ggagtattaa atgcttcaag aaagaaaaga atagctaaag
    13081 gtagtggaac tagtgtacaa gatgtaaata gacttataaa acaacttaat gaaatgaaaa
    13141 aaatgatgaa gatgtttgct ggttcacaaa agagtatgaa gaaaaggggc ggcttaggcg
    13201 gtttaccttt ttttaaataa aatataaaaa agtaaaaatt attcggaggt gactgtaatg
    13261 gcagttaaaa taagattaaa aagaatggga gctaacaaaa aacctttcta cagaatagta
    13321 gtagctgatt caagagctcc aagagatgga aaatttatag aagaaatagg atactataat
    13381 ccaatttctg aacctaaaca agtaagaata aatgatgaaa aagctataaa atggttagct
    13441 actggtgctc agccaacaga agtagttaaa aaattacttg taaaaaacgg agtaatagaa
    13501 aaattcgaag cttctaaaca agcaaaataa ttttttcttg gcaattagtt taagtaaggg
    13561 ctcattgcta aggttacttt aggagatgaa atatatgaaa gagctagttg tagatatagc
    13621 taaggctcta gttgataatc ctgactcagt tgttgttgag gaatttgaag ataatgatgg
    13681 tatcgtctta aagcttacag ttgctcaaga ggatatgggt aaggttattg ggaagcaggg
    13741 aagaatagct aaagctataa gaactgtcgt aagatctgtt gcaaatagag aaaatataaa
    13801 agtttctctt gagatagtat aaaataggag gattaggtat aatgcctagt cctttttata
    13861 taaatgtgta gtttaagatt ttatgataat atgaccacat aagattaaat tttttaagaa
    13921 ggtgataaca tgaaagaaaa attaactcat tttaaggtag gtcaaatagt aaatacacaa
    13981 ggtctaaaag gtgaagtaag agtttatcca cttactgatg atatagagag gtttgatgaa
    14041 ttaaaagatt tctatctggg aaaagattta aatgataaat tggctgttga aaaagtaaga
    14101 tacaaaggtt ctatggttat aatgaagata aaaggcatag acagcataga gaaagcagaa
    14161 aaactaaaaa ataaatttat gtatgtatct agagaagata gcagagagct tgaagaaggc
    14221 gaatttttta tagcagatat gattggaatg gaagttttaa ctgtagatgg aaaatatgta
    14281 ggaatactag aagatgttct tcaatactca gcaaatgatg tctatgtagt aaaaggtgag
    14341 gaagataaag aatttatgat accagctata aaaaaatttg tacctacaat agatattgat
    14401 gaaagaaaaa tgattataga ccctattaag ggtatgatag attaggtggt aatatgagat
    14461 ttcatataat gacacttttt ccagaaatat ttaattcgta catggatgaa agtataatga
    14521 agagagcagt tgaaaaaggt ataattgagg ttcatatata taatataagg gatttttcta
    14581 ataataaaca taaaaaagta gatgattatc catttggagg aggagcgggg atggtaatga
    14641 ctcctcaacc tgtatatgac acatataagc atataataac aactcacaat ataaatagtc
    14701 caagtgtaat ttatcttact ccaaaaggca aggtatacaa tcaatctata gcaaagcagt
    14761 tgtctttaaa agaagatata atactactat gtggtcatta tgaaggaata gatgagagaa
    14821 tcattgattt aatagttact gatgaaattt caattggaga ttatgtatta actgggggag
    14881 agctcccagc acttataatg atagattcta tatcaagatt aataccaggt gtattaaacc
    14941 aagaagaatc atttgaagaa gaatcattta aagataactt attggaatat cctcattaca
    15001 ctagacctag agattttgaa ggattaaaag taccagaagt gcttttatca gggaatcata
    15061 agaagataga tgagtggaga agagaagaat ctataaggat aaccaaagaa agacgatttg
    15121 atttgtataa aaagtctaat gaaaaataaa aatataatga aaaattaata tatatgtata
    15181 caataaagta cctataaatg gaattaaaaa cataaaagtg cttgtaataa ctattgaagt
    15241 atagtataat ataaaatgtt gaaaatacgg gcgttcctct gtttacactg gtcgggtagt
    15301 taagaacgtc tatgatatct aggaggaaga aagatgaatg aaatgctaag agcaattgag
    15361 caagaacaat taaaaaatga agttcctaat tttggacctg gggacacagt taaagtacac
    15421 gttagaatta tagaaggaaa aagagaaaga atacaagtat tcgaaggtgt agtattaaag
    15481 agacaaggtg gaggagcaag agaaactttc acagttagaa aaatgtcttt caatgttggt
    15541 gtagaaagaa ctttcccagt tcactctcca aaaatagaaa aaatagaagt tactagaaaa
    15601 ggtaaagtaa gaagagctaa actaaactac ttaagaggta gagtaggtaa agctgctaag
    15661 ataaaagaag ctagaaataa ataattaaaa actttactta taaatggggg gctgatttat
    15721 cagtcctctt tctattttaa tgaaaattac agcaaataat tatactagaa ataaatagaa
    15781 atatttgttg aaaatataca ttatttatct atgaatttat agcttttaaa atgaaaaata
    15841 aaattggtaa atattacatt atattttgta acgatattaa aaataagaga acagtattac
    15901 gtaaagtatg acgttaaaaa gatgacaata ttatagtttg tgaagatata tttttttagt
    15961 ggaattagga tataataata taatattaat aaggagtgat acatgtgtca aatgaatacg
    16021 aagagtatct tatggatgac aatctacata taaactggta tccaggtcat atgaaaaaaa
    16081 ctaaggagtt agtaaaaaat aacttaaaat tgattgatgt agtaattgaa cttttggatg
    16141 ctagaatccc gtttagtagt aaaaatccag acatagacag gcttgtagga gataagccaa
    16201 gagttgtcgt acttaataag agtgatatgg cagataggga taaattgaat caatggattg
    16261 aatactataa gaaaattaat ataaaagcta taccagtaga tacaataaaa ggtgttggta
    16321 taaataagat aatcgaagaa tgtaaaaatg taactagaga aaaaatgagt tctttaaaag
    16381 acaaagggag aaaagaaaga gctatacgaa taatgatagt tggagttcca aacgttggta
    16441 aatcatctct tataaacaaa ttaacaggta gaaaaagcac tcaaactgga gataagccag
    16501 gagtaacgaa agggaagcaa tgggttagat taaagggaaa tcttgaactt ttagacacac
    16561 ctggaatact ttggcctaag ttcgaagacc aagaagttgc attaaattta gcatttagta
    16621 gagctataaa ggatgagatt ttagatactg agactttggc tttaagactt atagaaaaac
    16681 ttatgaaaat agaacctgag aagttgaaag ccagatataa gttagattgt ctaggagaaa
    16741 ctccaattga aactatggat atgattggcc ataaaagagg gtttataaca ggtaaaaaag
    16801 aattagatta tacacgcata gccacaactg tattaaatga atttagagat ggaaaaatag
    16861 gaaatataac attggaagtt ccagaaaacg ttaaaagata gattttaaaa caaaatcaaa
    16921 aatttaatgt taaaaattta aaaaagcctt ttaccaaagg cttttttatt tttttgaaat
    16981 atagacaaat atctccatat aaaagtataa attaggttag aaaaccttta aaaatattaa
    17041 tttaagacaa atatacctaa tattttgaaa attaaaatga gtatgattat ttaaaaattt
    17101 agcatattga cttgcttgtt tcgtataata tataataagc acaaataaat acaatatatt
    17161 aaaaatttaa tttatttaat tttattaatt taataataag ttcttattgt ttaattcaac
    17221 ttatttatgc aagcgtaaac aatcgagaca ttttattaat ttaataaggt taaaaagggg
    17281 gattttataa tgggtaagac caaggatgtt atagtatttg gatttgcgtt atttgcaatg
    17341 ttctttggag caggtaactt aatatttcca ccttacttgg gaataattac aggtccagaa
    17401 tggttaattg ccttcttagg atttacgttt gctgatgctg gattggcact tctagcagta
    17461 atggctacag ctaaattcga tggaaacgta gttgaaatgt ttaaaagatg tggtatgaag
    17521 ttagggattc ttataggttg tgcagatatt ttatgtatag gaccattctt agctatacca
    17581 agaacaggtg caacaactta tgaaatgggt ataatgcctt tatttggaac ttcaattcca
    17641 gtgttattat tctgtatact tttttttgca atttcatatg tattaacaat aagaccttct
    17701 aaggtcgtag atatagttgg acagttttta actccagctc ttctaatagc tttagcattt
    17761 ataattataa aaggtattat atcacctctt ggagatatag tagataaacc tatgatacct
    17821 aatgtatttg cagaaggtat aggtcaagga tatcagacaa tggatgcttt tgcagcaata
    17881 gcacttgcat cagtgctaat agtatcttta aatgataaag gatattctac tattagtgac
    17941 aagttaaaaa tgattggtaa ggcgggcgtt cttgcttgtg gaggattagc tttagtttat
    18001 ggaggattat gtttccttgg agcaactgta tcaacaatgt atggaacaga tgcagtacaa
    18061 tcacaagtaa tagtaaatat aactgaagga ttgcttggaa atgttgggaa agtgatttta
    18121 gcagtagtag tgtcattagc atgtttaact acatctatag gtcttacatc tgctacaggt
    18181 caatatttct ctagacttac aaaagggaag ttgagttatg aaaaaatagt attagctgta
    18241 tctgtgttca gtgcagtagt agcatctttt ggtgttggaa ctataataaa gatagcatct
    18301 cctatattaa gtatagttta tccaccatca attgttttaa taattttagc tttcttcaat
    18361 gaaaaaatta agaatgataa tgtgtacaaa ggagcagtat atatgtcatt gcttgtaagt
    18421 atactaacag ttatatctag ttatggagta gctgttccag tagtaaattc tttacctctt
    18481 agttcattag gatttaactg ggtggtgcca gttataatag ctggaataat tggtaatttt
    18541 ataccatcaa agagtcaatc taatacactt ggaacaaact aagttttgtt taattaaaat
    18601 ataatcaatt gtaaataaat gagttaacta aaagtaaaag gagttatttg tataaataac
    18661 tccttttatt tttagattct at
//
LOCUS       DAEPXF010000071        18632 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain HB27
            SAMN13376389-rid12479423.denovo.075, whole genome shotgun sequence.
ACCESSION   DAEPXF010000071 DAEPXF010000000
VERSION     DAEPXF010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13376389
            Sequence Read Archive: SRR10518432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 18632)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18632)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001069624.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001069624.1
            Genome Coverage       :: 315x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/15/2021 01:54:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,165
            CDSs (total)                      :: 4,076
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 89
            rRNAs                             :: 10, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 10 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 19 of 69
            Pseudo Genes (incomplete)         :: 44 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 10 of 69
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18632
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13376389-rid12479423.denovo.075"
                     /strain="HB27"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="China: Hebei"
                     /collection_date="2017"
     gene            103..1293
                     /locus_tag="KRL67_002083"
     CDS             103..1293
                     /locus_tag="KRL67_002083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HBH1522960.1"
                     /translation="MISNKMQTLVANSSVIRAMFEEGKKLSDIYGEENVFDFSIGNPS
                     VEPPETIKAVINDILNEESPNLVHGYMNNSGYEDVRDAIAEHINKKDGLNLTRENLIM
                     TCGAAGGLNIILKTLLNPGDEVIAFAPYFGEYKNYTENYDGKLIEVPTNIETFEPDLD
                     ALKNAITPKTRALIINTPNNPTGVIYSEELLKNLGELLDSKQKEFNTNIYLISDEPYR
                     EIIYDGAKVPCVLKYYRNSFIGYSYSKSLSLPGERIGYIVANGRMDDFDDVMSSLNVA
                     NRILGFVNAPSLFQRVIARSLDAEVDVNIYKKNFDLLYNSLIDMGYSCVKPNGTFYLF
                     PKAPIEDDKKFCNDAKQFNLLLVPGSSFGCPGHFRVSYCVSYDKVKSSLPAFEKLAKL
                     YNLK"
     gene            1463..2425
                     /locus_tag="KRL67_002084"
     CDS             1463..2425
                     /locus_tag="KRL67_002084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HBH1522961.1"
                     /translation="MKKGYVYIILTTLFFSSMEISLKTVTNDFNPLQITLSRFFVGGL
                     VLLPFAVKRLRALSLSITKSDLKYFAFLGFMCVVVSMSLYQLAVLNTKASVVAVLFSC
                     NPVFVMLLAYLILKEKIYRHNVISLILEVLGIIVIINPLHTKLTLSGIFLTLSSAIIF
                     SMYTVFGKRKTLKFGGIVVTCFSFIFGSLEMLILVLLTKINFIADILNKNKLHVFANI
                     PIFSGYTLHNMPIMIYVFVFVTGVGYALYFMAMEATSTSLTSLVFFFKPVISPILALL
                     ILKEIIPINMVIGILLIVIGSIISIIPTIITQMHNKHVEALYKE"
     gene            complement(2546..3208)
                     /gene="zmp1"
                     /locus_tag="KRL67_002085"
     CDS             complement(2546..3208)
                     /gene="zmp1"
                     /locus_tag="KRL67_002085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metalloprotease Zmp1"
                     /protein_id="HBH1522962.1"
                     /translation="MRPSKKLLIAIISIFLISSVPVSAHADSTTIQQNKDTLSQIVVF
                     PTGNYDKNEANAMVNRLANIDGKYLNALNQNNLKIKLLSGKLTDEKEYAYLKGVVPKG
                     WEGTGKTWDDVPGLGGSTVALRIGFSNKGKGHDAINLELHETAHAIDHIVLNDISKSA
                     QFKQIFAKEGRSLGNVNYLGVYPEEFFAEAFAYYYLNQDTNSKLKSACPQTYSFLQNL
                     AK"
     regulatory      complement(3308..3393)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            complement(3579..6497)
                     /locus_tag="KRL67_002086"
     CDS             complement(3579..6497)
                     /locus_tag="KRL67_002086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021362860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SrtB-anchored collagen-binding adhesin"
                     /protein_id="HBH1522963.1"
                     /translation="MKKENRKTLLISLIMILSMLVSTIYPTVSYASELGENSQIQSSS
                     TNSSTGEDKESDNKKPEQIPEKDKATDNKKPEQTPEEDKSTDNKKPEQTPEEDKSTDN
                     KKPEQTPEGEKPIDNKKPEQIPEEDKSTDNKKSEQALEDEKPLDNKNTEKTPEEDNLL
                     EDENLLKVLEEELNEENEDYGFVVKINNNTIETESMKKISFNLTYTPTSKGIQAGDSI
                     TFKVPDVFNKVNLDYTSECFDKTESNGEYTLTFRELPNGQSVMQGKIGLEAYVKKVDE
                     ETNAKIHIETTGKIESGSGDVDVEIKPGDKEDIPDAKGTLKKLVEGRKSTTVFMPVKN
                     KDINYSIQVNEKQEELKDITLYDELPEGLTLINGSVSVVTSDGKELSDFNIEQNENSI
                     SVDFGNIDKSYTVKYKARISDKNVKHDSKYKNVASIKSDGKKIQEDDATVSVFDRGDN
                     YLLTKGHSGATNITQVGQVINYQISVNDDKSPISNVVITDNIPEGMRLTTSGEAGHDF
                     RVVEVPMNGSWTPWSKEKIANNISYKIEEKRNESGQVDKVITGFTINLSKEEVESKFF
                     IAYTLKVISIEDSYINRAVLDANNSKIDKNDEINFKKNSGLISAKKEVDKKVLNSSDN
                     QIVKYKINMSTYGVYDAGQVNLLDEVNQALKISNIKYSDNLELKKEAGDGKNTIRLVN
                     KYEFKQIKEGEPVQSWVTFDADFTNVKVGETIQNVAQINGSSPPGVETTKQGYAFEAK
                     KVDALDKNVLSGARFNLEDAFGNIVVKDLVSDEDGVIQSSVKKTGIYYLVEVMAPSGY
                     EKLKEKVKVEIGNKDIGKIVDIGNIENIRQENPPVNPPVPPNTDEPIVNPPVPPSTDK
                     PRKPSSPSDTDETAVINPPVPPSEDMINPPIPEIVNPPIPPSEEMIETPVKQIVPIPE
                     VVKPSVSEEKNNKVKDDTLVNPPVPPKTGDSTTIIGEILLVIVAIVGLIILRRNKNTN
                     "
     regulatory      complement(6615..6700)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01786"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class II; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01786"
     gene            complement(6828..8006)
                     /locus_tag="KRL67_002087"
     CDS             complement(6828..8006)
                     /locus_tag="KRL67_002087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HBH1522964.1"
                     /translation="MFSKILVKTFIRDSENVQNTDVRNKYGYVAGVVGILSNLLLFVI
                     KVFIGMLTSSIAIMADAFNNLSDMASSAITMIGFKLASKPADKEHPFGHGRIEYLSAL
                     IVAFMVMLVGLQFVKSSIERIVNPIPVKFEVIPLILLIASIMIKIWLSRFNKFMGNKI
                     DSSALKAVSLDALGDVFTSSCVVISFIVARFTNFPIDGYVGIVVSLVILYAGFSLVKD
                     TINPLLGEAPDEEMVNSIIELLLSYKYIIGTHDLIIHNYGVGRCIASIHAEIPSNIDI
                     MEIHEIIDTAEREISEKLDIYLVIHMDPICLEDKEVMSAKKELEEILKKNSLVKSMHD
                     FRIVGKGSKKNLIFDIVVNPSEFSKNMSEDDLKEDITKLVKEINPEYNCVIVVDKDFI
                     "
     gene            complement(8550..9938)
                     /locus_tag="KRL67_002088"
     CDS             complement(8550..9938)
                     /locus_tag="KRL67_002088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HBH1522965.1"
                     /translation="MPVNSFDNYYMSWKPNKNRLSNPLYLSLAKCMEEDIEDYVLKEG
                     IKLPPQRELADFLDISLNTVTKAYNLCKEKGLLYGVAGSGTFVSQNAGLSKTILSEEK
                     INKNIDMAMLVPFYNHNKIIQSLAIEILQQPNADKYLEYTHPLGNCIQKQAAKKYLNK
                     FNVNAKEENIIIANGTQNALMLTLVSLFKPGDKIATDTYTYANFIGLANLLHIELVSI
                     NGDDKGMLPESLDNLCKIRDIHGVYLSPSCSNPTNITMRKNRREEIAHVIKKHDLILI
                     EDDIYAFILDNPLTPIYSVIPEQSVYIGGISKSICPGLRIAYMSFPDKFRKKLENGVY
                     SCNLKTSSLNVEIATQLILKDMYEDIICDKRRMAIRRNKIFKKYFPDLDEGYNENSYY
                     QWICLPKGITGRIFELEGYKRNLKLYSSERFSVGDTQNKFFIRLATCSSKNVEEFENG
                     LSILSNIIDECK"
     gene            10085..11485
                     /locus_tag="KRL67_002089"
     CDS             10085..11485
                     /locus_tag="KRL67_002089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HBH1522966.1"
                     /translation="MKNKKYISPILENIRNTDFTNDLTIAHNYAVEYINTVDNMRVYP
                     DKKALNNLSRFDENLCKTPTSNAEILHQLGKYGSKATVAQSGGRYFGFVCGGILPSSL
                     CSKWITDVWDQNPALYVLSPISSKLEDICEKWIVKLLGLPENTSVGFVSGSSTAIICG
                     LLAARNYLLNKLEYNVSKKGLFDSPKIKVVLSEGAHSTIFKSLSIIGLGQESIIKVPM
                     DEQGRILVAKIPPLDNSTIVILQAGNVNTGSFDNFEEVCKKAKDVGAWVHIDGAFGLW
                     AAANNTMSDLSKGIELADSWNMDAHKTLNSPYDNGIVLCRHRESLVNAMHMTDDYIVL
                     SKNRDSMMYTMEMSRRARAIDIWATLKGLGSDGVSELVEELHYKAKYFSDCLIKHNFE
                     ILNEVVFNQVLAYFKNDTATEELIRRIQESGVCWLGGSKWNGKSVMRISISSYKTTYE
                     DIDICIEEIVKLSKTL"
     gene            complement(11543..14317)
                     /locus_tag="KRL67_002090"
     CDS             complement(11543..14317)
                     /locus_tag="KRL67_002090"
                     /EC_number="7.2.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-translocating P-type ATPase, PMCA-type"
                     /protein_id="HBH1522967.1"
                     /translation="MRYYNKPTKEVLKYLKTNPEIGLDDDEVEERKLRYGLNEFTIKE
                     GRTFWDELGESLTEPMILILIGAAVISSFVGELHDALGILGAIFIGISIGIITEGKSK
                     KAAHALSKLTENIEVKVLRNGKIIKISKNDLVPGDIVYIETGDMIPADGRLIQSINLK
                     LREDMLTGESDDVAKNADAVLDMEVVYSKTEIIEQDAIPAKQVNMVFGGTLVAYGRGI
                     MVVTHTGDKTEMGKIAQNLSNEEQQTPLQIKLGKLGAKIAGISGVIATLLCMFMIIQM
                     QRKGMLILDTSSVLSFLQSLEPAKNAYMVCIALIVATVPEGLPTMINITLAITMQKMA
                     KINALVTKKEACETIGSVSVICSDKTGTLTQNKMMVEVAYVDGKYISGGEYQSNSYFE
                     QNCIVNSTADIEKEDNSFKYIGSATECALLLYHNDKNYNEMRKQTYLISQIPFSSEEK
                     KMSTLIRQEDSDILLSKGAPEVLLKKCSYVQQGKNIVPITPKIEKSILDEIKKLQIKS
                     MRTLGFAYKKMSKSKTEVAITSEGELNLIGNQRGYMKEDNLVFSGFVGIVDPLREGVK
                     ESIDKAFNAGVDVKMLTGDNINTATAIGNELGLLNDGKKAVEATYIDVLTDKELREEI
                     KGISIVARSKPDTKMRIVSALQKSGEVVAVTGDGINDAPALSQADVGIAMGISGTEVS
                     KNAADIILTDDSFSTIVEGIKWGRGIYENFQRFIQFQLTVNIVAFIIAIISQLTGKDM
                     PFTTIQLLWVNIIMDGPPALALGLEPVRDYVLKRKPINRHSGIIARSMFVNIIINAIL
                     IITIVFTQSAFNILGATSEEQGTVIFSLFAFSALFNALNCREFGLNSTIPNFFKNKLA
                     LQIIVVTGIIQIIFTQVFQSFFNSVSLDFDMWIKIILFASTILLSNEFVKLILRTMKN
                     SRTINFNSKN"
     gene            complement(14489..15745)
                     /locus_tag="KRL67_002091"
     CDS             complement(14489..15745)
                     /locus_tag="KRL67_002091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HBH1522968.1"
                     /translation="MLKTTKISDPELYKIVADELVRQEHNIEMIASESTAPTEVLELS
                     GCVFTNKTEEGYPGARFQAGSEEADKLEILAIKRAKEVFGAEHVNVQPYSGSTANYCV
                     YSSILKPNDTVLSMRLDQGGHLTHGSTVNFLHDIYKYEFYGVDPNTGRIDYDALEAKA
                     KECRPKLIIAGASSYPRLIDYERISKVAKEVGAYFMVDMAHVAGLVAAKVIPSPVPYA
                     DFVSSSTTKTFCGPRSGIVFCKAEHAKKLDKGVFPGTLGSIHLNTVAAKAFSLLYLST
                     DKFKKIMEQVVVNAQTLASELISHGFSIVSGGTDNHIVMVDLRSKNLTGKQFEKALEY
                     VGITVNKNVIPDDPQSPFVTSGVRIGLTSISQRGLKEKEVVQIAGIMNKVAENIDNKE
                     ILDECKSEAQELISKFPLYPEGYFED"
     gene            complement(15817..17319)
                     /locus_tag="KRL67_002092"
     CDS             complement(15817..17319)
                     /locus_tag="KRL67_002092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbgT family transporter"
                     /protein_id="HBH1522969.1"
                     /translation="MRALNVVERAGNKLPDPVTIFLLLCIIVVILSAVISNLGVEEIH
                     PSTKEVVKVVNLLEKEQIQSYLGSIVTNFQSFAPLGLVLVTMLGAGVAEKSGFMEVLM
                     KKGISKVPQKLVTVAIVFAGMLSHTAADVGFIILPPLAALVFLGIGRHPLVGMFAAFA
                     GVAGGFAANVMLSTTDVLLAGFTIPAAQMMDPSYQGNPAMNLYFAFISAIVLTFAGAF
                     VTEKFIAPRFTKYNDSTNDKDIQELSELENKGIKYALLSLLVVVVVIVALCIGDNAFM
                     KDPETGSILSSNAPLMKGIVPIITIIFLTPGLVYGKVSKKIKSDKDLVSMMGSSMSDM
                     GGYIVLAFIASQFINLFNLSNLGTILSITGAKLLAESGIPSYGLIIGFILLSGFINLF
                     VGSASAKWAILAPIFVPMFMLLDFNPALTQIAYRIGDASTNPISPLFPYFPVILAFAR
                     RYDKDIGIGTVISNMIPYSVVFTLIEIIILLLFMGIGIPLGPGGGISYVL"
     gene            complement(17410..18600)
                     /locus_tag="KRL67_002093"
     CDS             complement(17410..18600)
                     /locus_tag="KRL67_002093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HBH1522970.1"
                     /translation="MHELILISEKYKEDILDFFTDIHMHPELSFKEFRTTKAIKDLLV
                     SLDIEILDLGMETGVVGLLKGKYDGPTVALRGDIDALPIYEEVDIEYKSRYDGIMHAC
                     GHDIHTSCLVGCAYVLSHIRDSLHGNVKFIFQPAEEVNKGAKMLVERGVMENPKVDAI
                     FGLHNHPDIPCGKIGVKLGGLMAAVDTIKIEVNGFGGHGGIPNRTIDPIVASSAIIMG
                     IQTIVSRNISPLESAVISIGTINGGTANNVISEKVDMTGTCRSFSNEVRKKISENLEN
                     IVCEIARGYQATAKLDYLFDLPAVINSKEMYAIACKSVCDLYSEDAIVDPIPSTGGED
                     FSIFMEKAPGFFYWLGVGNKEQDCIYQWHNPKFKADKNSILVGTNVLCQSVINYMDKL
                     KNKI"
BASE COUNT         5887 a         3155 c         2353 g         7237 t
ORIGIN      
        1 ttatattttc aaacaaataa tttttcgaaa attgtctaaa ttcagtaaaa atatggtaat
       61 atatacataa gcaatataaa tctataaaaa ggagtgttac atatgatatc taataaaatg
      121 caaactttag ttgcaaacag ttctgttata agagctatgt ttgaagaagg aaagaaactt
      181 tctgatattt atggtgagga aaatgttttt gattttagta taggaaatcc aagtgttgaa
      241 cctccagaga ctattaaagc agttataaat gatattttaa atgaagaatc tccaaattta
      301 gttcatggat atatgaataa ctcaggatat gaggatgtaa gagatgctat tgctgaacac
      361 ataaataaaa aggatggatt aaatcttaca agagaaaatt taattatgac ttgtggtgct
      421 gctggtggtc taaatattat tttaaaaact cttttaaatc ctggtgatga agttattgct
      481 tttgcaccgt attttggcga gtataaaaat tatacagaaa actatgatgg aaaattaata
      541 gaagttccta caaatataga gacttttgaa ccagatttag atgctctaaa aaatgctatt
      601 actccaaaaa caagagcttt aataataaac actccaaata atcctactgg tgtcatatat
      661 tctgaagaat tattaaaaaa tcttggagaa ttattggatt caaaacaaaa agaatttaat
      721 acaaatatat acctaatttc tgacgagcca tatagagaaa taatttatga tggtgccaaa
      781 gttccttgtg tactaaaata ctatagaaat tcatttatag gatactctta tagtaaatca
      841 ttatctctac ctggtgaacg tataggatat attgttgcta atgggcgaat ggatgatttt
      901 gatgatgtta tgtcttcatt aaatgttgca aataggatac ttggatttgt aaatgctcct
      961 tctctttttc aaagagttat tgcaagaagt ttagatgctg aagtcgatgt aaatatttat
     1021 aaaaagaatt ttgaccttct atataatagt ttaatcgata tgggatattc ttgtgttaaa
     1081 cctaatggta cattctattt gttcccaaaa gctccaattg aagatgataa aaaattctgc
     1141 aatgatgcaa aacaatttaa tttattatta gtaccaggtt cttcttttgg ttgtccaggt
     1201 cattttagag tatcatattg tgtatcttat gataaagtta aaagctcttt gccagcattt
     1261 gaaaaattag ccaagttata taatttaaaa taactaaatc aaagtaattc aaataattaa
     1321 ctaccttagt atagaatact tacattctat attaaggtag tttataattt tatgtactat
     1381 attttataca taccatatta ttccaatatg cttgaatatt cttatctata gtactaatat
     1441 aaattacaag gaggtgtttg acttgaaaaa aggttatgtt tatataatac tgactacctt
     1501 attttttagt tcaatggaaa ttagtctaaa aactgtcact aatgacttta atcccttaca
     1561 aataacttta tcaagatttt ttgttggcgg tttagtctta ctaccttttg ctgtaaaaag
     1621 attaagagct cttagtcttt ctattacaaa atcagactta aaatactttg catttttagg
     1681 ttttatgtgt gtagtagtaa gtatgtcatt gtatcaactg gctgttctaa acacaaaggc
     1741 atctgtagtt gctgttttgt tcagctgtaa tccggtattt gtaatgcttt tagcttactt
     1801 aattttgaag gaaaaaatat ataggcacaa tgtaatttca cttatattag aagttcttgg
     1861 aattatagtt ataataaatc cattgcatac aaaactaact cttagtggaa tattcttaac
     1921 attatcttct gctattattt tttcaatgta tactgtattt ggtaaaagaa aaaccttaaa
     1981 atttggtgga atagttgtaa cttgctttag ctttatattt ggtagtttgg aaatgttgat
     2041 attggtttta ctcactaaaa ttaattttat cgcagatata ttaaataaaa ataaacttca
     2101 tgtatttgca aatatcccta tttttagtgg atatacctta cacaatatgc ccattatgat
     2161 ttatgtattc gtatttgtta caggtgttgg ttatgcactt tactttatgg caatggaagc
     2221 tacatctact agtcttacat ctttagtatt cttctttaaa ccagtaatat ctccaatact
     2281 agcattatta atacttaaag aaattatccc tataaacatg gttataggaa ttttattgat
     2341 agtgattggt tcaataattt ctattatacc tacaataatt actcaaatgc ataataaaca
     2401 cgttgaagcc ttgtataagg agtagtaatt gataatctat caagttatac ataagagaaa
     2461 atttattaga tttaaacgca atataaaaga gatgctatta taatagcatc tcttaattta
     2521 tatttatttt aaatttatta aagctctatt tagctaggtt ttgcaaaaag ctataagttt
     2581 gtgggcaagc tgattttaac ttactatttg tatcttgatt taaatagtaa tatgcaaatg
     2641 cttctgcaaa aaattcttct ggatatacac ctaagtagtt tacattacct aaagaacggc
     2701 cttctttagc aaatatttgt ttgaattgtg ctgatttaga tatatcattt aatactatat
     2761 ggtctattgc atgtgctgtt tcatgtaatt ctaaatttat tgcatcatgc cctttacctt
     2821 tgttactaaa tcctattcta agagctactg tacttccacc taaacctgga acatcatccc
     2881 aagtttttcc agtaccttcc catcccttag gaactacacc ttttaaataa gcatattctt
     2941 tttcatctgt taactttcca cttaataatt ttatttttaa gttattttga tttaatgcat
     3001 ttaaatactt tccatctata ttagctaatc tatttaccat agcattagct tcgtttttat
     3061 cataatttcc agttggaaaa actactattt gactaagtgt gtctttattt tgttgtatag
     3121 tagtactatc tgcatgtgca cttactggaa ctgaacttat taagaatatt gatattatag
     3181 ctattaataa ttttttactt ggtctcatat tattccctcc tttgagttag gccgggagta
     3241 ataaatttca aaataaaaaa gatacccaag agtatctttg tatatacaaa aagaaatttt
     3301 tactcttttc ggcaactggc tggcatggtt tactaaactt tagcccctat agctttgcgt
     3361 gactaggttt ccctagtttt gcctcattta attttatatc tatattatag cttatatctt
     3421 cttaattgtc tatatccctt tggaactttt ttctaaaaag ttattatttt tttacataaa
     3481 tcttcaaaaa aaaatatctt ctaacaatat tttcattatt aaatattatt ttagaagata
     3541 ttttttaata taacttacat tttttataat gttagggtct aatttgtatt tttatttctt
     3601 cttaatatga taagtcctac aatagctact ataactaaaa gtatttctcc aataattgta
     3661 gtactatccc ctgtttttgg tggtactggt ggatttacca aagtatcatc tttaacttta
     3721 ttattttttt cttctgacac tgatggcttt accacttctg gtattggaac aatttgttta
     3781 acaggagttt ctatcatctc ttctgatggt ggtattggtg gattcactat ctctggtatt
     3841 ggcggattta tcatatcttc tgatggtggt actggtggat ttataactgc agtttcatcc
     3901 gtatcagatg gacttgatgg tttccttggt ttatctgtgc ttggtggcac tggtggattt
     3961 actattggtt catctgtatt tggcggtaca ggtggattta ctggtggatt ttcttgtctt
     4021 atgttttcta tatttccaat atctactatc ttaccaatat ctttattacc aatttcaact
     4081 ttaacttttt ccttcagttt ttcatatcca cttggagcca ttacttctac taaataatat
     4141 attcctgttt tttttacact agattgaatt acaccatctt catcactaac taaatcctta
     4201 actactatat ttccaaatgc atcttctaaa ttgaacctag cacctgaaag tacattttta
     4261 tctaaagcat ccactttttt agcttcaaaa gcataacctt gcttagtagt ctctactcct
     4321 ggaggacttg aaccattaat ctgagccaca ttttgtatag tctctccaac ttttacattt
     4381 gtgaagtctg catcaaacgt aacccaactt tgcacaggtt caccttcttt tatctgttta
     4441 aattcgtatt tatttactaa tcttattgta ttttttccat ctccagcttc ttttttaagt
     4501 tcaagattat cagaatattt tatattagat atttttaaag cttggttcac ttcatccaaa
     4561 agatttactt gtcctgcatc ataaacccca taagttgaca tattgatttt gtatttaaca
     4621 atctgattat cactactatt taaaaccttt ttatcaactt ctttttttgc gcttattaat
     4681 ccagaatttt tcttaaaatt tatctcatca tttttatcaa ttttactatt attggcatca
     4741 agaacagctc tatttatata gctatcttca atactaatta cctttaatgt ataagctata
     4801 aaaaactttg attccacttc ttctttactg agattaattg taaatccagt tataacttta
     4861 tctacttgcc cactttcatt tcttttttct tctattttat atgaaatatt attggcaatt
     4921 ttttctttac tccaaggagt ccagctacca ttcattggaa cttctacaac tctaaaatca
     4981 tgccctgctt cccctgaggt agtcaatctc ataccttctg gtatattatc tgttataaca
     5041 acattactta ttggtgactt atcatcattt actgaaatct gatagtttat tacttgacca
     5101 acttgagtaa tatttgttgc tcctgaatgt cctttagtga gaagataatt atcccctcta
     5161 tcaaatacac ttacagtagc atcatcttct tgtatcttct taccatcaga ttttatacta
     5221 gctacattct tatatttact atcatgtttt acatttttat cactaattct agccttatac
     5281 ttaacagtat acgatttatc tatatttcca aaatcaactg aaatagagtt ttcattctgt
     5341 tcaatattaa aatcactcaa ttcttttcca tcacttgtta ctacacttac agaaccattt
     5401 atcaatgtca atccttcagg aagctcgtca tacaaagtaa tatcttttag ctcttcctgc
     5461 ttttcattta cttgtatact gtaatttatg tctttatttt taactggcat aaataccgta
     5521 gttgattttc ttccttcaac aagcttctta agtgttcctt tagcatctgg aatatcttct
     5581 ttatctccag gctttatttc tacatctaca tctccacttc cactttctat ttttccagta
     5641 gtttcaatat gtatttttgc attagtctct tcatcaactt ttttaacata tgcttccaaa
     5701 cctattttcc cttgcattac actttgacca ttaggtaatt ctctaaatgt aagtgtatac
     5761 tctccattac tttctgtttt atcaaagcat tcacttgtat aatctaaatt aactttattg
     5821 aatacatctg gtaccttaaa agttattgaa tcacctgctt gtattccttt tgatgttgga
     5881 gtataagtta aattaaatga tatttttttc attgattccg tttctattgt attgttatta
     5941 atttttacaa caaatccata gtcttcattt tcttcattaa gttcttcttc taaaactttc
     6001 aataaatttt catcttctaa tagattatct tcctctggag ttttttctgt atttttattg
     6061 tctagtggtt tttcatcttc taaagcttgt tctgactttt tattatctgt tgacttatct
     6121 tcttctggta tctgttctgg ctttttatta tctattggct tttctccttc tggggtttgc
     6181 tctggctttt tattatctgt tgacttatct tcttctggag tttgctctgg ctttttatta
     6241 tctgttgact tatcttcttc tggggtctgt tctggttttt tattatctgt tgccttatct
     6301 ttctctggaa tctgttctgg ttttttattg tcgctttcct tatcttctcc agttgatgaa
     6361 ttagttgaac tactttgaat ctgactattc tctcctaatt ctgaagcata agatacagtt
     6421 ggatatattg tagacaccaa catacttaaa atcataatta aactaattaa taatgtcttt
     6481 ctattttctt tcttcatata aatttcaccc ctttaggtta tctataattt ataatctaat
     6541 cttccattaa tatcgtatat aaagtaattt ttactaaaaa attatacaaa aaaagctatg
     6601 aaaataaata aatttatatt catagccggt tgcaccactg actcagtata tccataaggt
     6661 gcccaataat agaatatact tcaacatctc tataatattt cataaaaaca attaaaattg
     6721 ttttatttat attaaaaata caattatata cttctattat attatgcctc tatactattg
     6781 tctatccttt ttacattttt tacctttatt atgaggttaa ttaataatta aataaaatcc
     6841 ttatctacaa caattacaca attatattca ggatttatct ccttaactaa ttttgttata
     6901 tcttctttta agtcatcctc tgacatgttt ttggaaaatt cggaaggatt aactactata
     6961 tcaaatatta aattcttctt gcttcccttt ccaactattc taaaatcatg catagatttt
     7021 acaaggctat ttttcttaag tatttcttca agttcttttt tggcggacat aacttcttta
     7081 tcttcaagac atattgggtc catgtgaatt actagatata tatctaattt ttcagatatt
     7141 tctctctctg cagtatctat aatttcatgt atttccatta tatctatgtt ggatggaatt
     7201 tctgcatgta tagatgcaat acatctaccg actccatagt tatgaataat taaatcatgt
     7261 gtacctatta tatatttata agataacaat agctctataa tagaatttac catttcttca
     7321 tctggtgctt ctcctagaag aggatttata gtatctttta caagagagaa accagcataa
     7381 agtatcacta atgatactac gataccaaca tatccatcta taggaaaatt agtaaatcta
     7441 gctacaataa atgatataac tacacaactt gaagtaaata catctcctaa agcatcaagt
     7501 gaaactgctt ttaaggctga agaatcaatt ttatttccca taaatttatt aaaacgactc
     7561 aaccagattt taatcattat agatgcaatt aaaagtataa gtggtattac ttcaaatttt
     7621 acaggaattg gatttactat cctttctata gatgacttta caaattgaag tccaaccaac
     7681 atcaccataa atgctactat taatgctgat aaatactcta ttcttccatg accaaaagga
     7741 tgttctttat cagctggctt acttgctaat ttaaaaccta tcatagttat tgctgaagat
     7801 gccatatcag aaagattatt aaatgcatca gccattatag ctatactaga tgtgagcatg
     7861 cctatgaata cctttatcac aaataaaagt aaattggata atatacctac tacaccagct
     7921 acataaccat acttatttct aacatctgta ttttgaacgt tttcgctatc tctgataaat
     7981 gttttgacta ggattttgga aaacatgaaa cttacctccc tgccctttat tttattaaca
     8041 aattttataa tctatatctt tatttatgat aacataattc gtatataaaa ttcttaaaaa
     8101 tcactctact ttaataaaat attattctat atatccattt ggatacaaat tattgtattt
     8161 tgtaaagttt ttataattta atatctgttt aatacattct atgtcaagtt ttacaaaaat
     8221 tatttattat gataatatca tttttcccaa gtattactta gaatatgatt tttttgtttt
     8281 gtctgtacat attttaaatt tatacaatat ttatctatat tttatataat ttataaactg
     8341 ctttactgca agtgaaatat tctttttata tcttaacaca atttcaatat ttcgataaac
     8401 tgaaacttcc aacttctttg ctacagtatg ataagggata tgttgtaaaa taagtcgtgg
     8461 taatttcaat atttctaact ctaacatata gctatttatt aaactctata ttgattattt
     8521 cacgatgtac ttggattatg gattaattat cacttacatt catctataat gttgcttaaa
     8581 atagataatc cattttcaaa ttcttctaca ttcttagatg aacatgtcgc taatctaata
     8641 aaaaatttgt tctgtgtatc acctacagaa aaacgttctg aggaataaag tttgagattt
     8701 cttttatacc cttccagttc aaatattctc cctgtaatac cttttggcaa acaaatccat
     8761 tggtaataag aattttcgtt atagccttca tctaaatcag gaaaatactt tttaaatatt
     8821 ttatttctcc taatggccat tctacgctta tcacaaatta tgtcttcata catatctttt
     8881 aaaattaatt gcgttgctat ttcaacattc aatgaagaag tttttaaatt acaactatat
     8941 actccatttt caagtttctt tctaaattta tctggaaaac tcatatatgc aattctaagt
     9001 cctggacaaa ttgatttaga aattcctcct atatatactg attgctctgg aataacagaa
     9061 tatataggtg ttaatggatt gtctagaata aaagcataaa tatcatcttc tattaaaatt
     9121 aaatcatgtt tttttataac atgtgcaatt tcttctcttc tattttttcg catagtaata
     9181 tttgtaggat tactacaaga aggagataaa taaactccat gtatatccct tattttacat
     9241 aagttatcca agctttctgg taacatacct ttatcatcac catttatgga aactaattca
     9301 atatggagta aatttgcaag ccctataaaa tttgcatatg tataagtatc tgtagcaatt
     9361 ttatctccag gtttaaaaag tgaaactaat gttagcatca aagcattttg tgttccattt
     9421 gcaataataa tattttcctc ttttgcatta acattgaatt tattaagata tttttttgct
     9481 gcttgttttt gtatacaatt acctagagga tgtgtatatt ctagatattt atcagcatta
     9541 ggttgttgca aaatttctat tgctaaactt tgaataattt tattgtgatt ataaaatgga
     9601 actaacatgg ccatatctat atttttatta attttttctt ctgagagtat tgttttactt
     9661 aaacctgcat tttgagatac aaaagttcca ctccctgcta ctccatacaa taatcctttt
     9721 tctttacaca agttataagc ttttgtaact gtattaagac ttatatccaa aaaatctgct
     9781 agttctcttt gtggtggaag ttttatcccc tctttcaaaa cgtaatcttc aatgtcctct
     9841 tccatacatt ttgctaaaga taggtataat gggttactta gtctgttttt gttaggtttc
     9901 catgacatat aataattatc aaaagaattt acaggcatat ttattcctcc taaatttctt
     9961 attattttaa ttactaaaat tgtacacagt acaatttctt aattgaatat aatataattc
    10021 gaatgatatt atcatattaa caatacaatt tagatgttac aattcttttt tataggagga
    10081 acttatgaaa aacaaaaaat atattagtcc tattttagaa aatatacgta atacagattt
    10141 tacaaatgat ttaacaatag ctcataatta tgcagtagaa tacattaata ctgtagataa
    10201 tatgagagtt tatcctgaca aaaaagcgct caataattta tcacgatttg atgaaaactt
    10261 atgtaaaaca ccaacatcaa atgccgaaat acttcatcag ctcggaaaat atggttcaaa
    10321 agctacagtt gctcaaagtg gaggacgtta ttttggattt gtctgtggtg gcattttacc
    10381 atcttcttta tgttctaaat ggataactga tgtttgggat caaaatcctg ctttgtacgt
    10441 attatctcct atttcatcaa agctagaaga catttgtgaa aaatggatag ttaaattact
    10501 agggcttcca gaaaacacat ctgtaggatt tgtaagtggc agctctactg ctattatttg
    10561 tggtttactt gctgctagaa attatttact aaataaatta gaatataatg tatcaaaaaa
    10621 agggttattt gattccccta aaattaaagt tgttttaagc gaaggagctc attctacaat
    10681 ttttaaatct ttatcgatta tagggcttgg tcaagaaagc ataataaaag ttcctatgga
    10741 tgaacaagga agaattttag ttgctaaaat tccacctctt gataatagta ccatcgtaat
    10801 tttacaagca ggaaatgtta atacaggttc ttttgacaat tttgaagaag tttgcaaaaa
    10861 agcaaaagat gttggtgctt gggttcatat agatggtgct tttgggcttt gggcagctgc
    10921 aaataataca atgtcagatt tgtcaaaggg aattgaattg gctgattctt ggaatatgga
    10981 tgctcacaaa acattaaatt ctccttatga caatggaatt gtactatgtc gccatagaga
    11041 atcattagta aatgcaatgc acatgacaga tgactatata gttcttagta aaaatagaga
    11101 ttctatgatg tatactatgg aaatgtctcg tagagctaga gccattgata tttgggctac
    11161 tctaaaagga ctaggtagtg atggtgttag tgaattagtt gaagaacttc attataaagc
    11221 aaagtatttt tctgattgtc taattaagca caattttgaa attttaaatg aagtagtatt
    11281 caatcaagta ttagcctatt ttaaaaatga cactgctaca gaagagttga tacgaagaat
    11341 ccaggagtct ggtgtttgtt ggttaggtgg ttctaagtgg aatggaaaat ctgttatgag
    11401 aataagtatc tcttcctata aaaccactta tgaagatata gatatttgta ttgaagagat
    11461 agttaaatta tctaagaccc tataatataa tttaaagact gcaaaatcgt aatatgcaga
    11521 tgattttgca gtcttataag aattaatttt tactattaaa gtttatagtt ctactatttt
    11581 tcattgttct aagtattagc tttacaaatt catttgacaa taggatagtg gatgcaaata
    11641 gtattatttt tatccacata tcaaagtcca aagaaacaga attaaagaaa ctctggaaca
    11701 cttgtgtaaa tattatttga attatccctg taactactat tatttgcaat gccagtttat
    11761 ttttaaagaa atttggtatt gtactattta aaccaaactc tctacaattt aatgcattaa
    11821 aaagggcact gaacgcaaat aaagaaaata taacagtacc ttgttcttct gatgtagctc
    11881 ccaatatatt aaaagctgat tgagtaaata caattgtaat tataagaata gcatttataa
    11941 taatatttac aaacatagac ctagctatta ttccagaatg tctatttatt ggttttcttt
    12001 tcaatacata atctctaaca ggttctaatc ctaatgccag ggctggagga ccatccatta
    12061 ttatatttac ccataataat tgtattgtag taaatggcat atccttgcca gtaagttgag
    12121 atattatagc aattataaat gctactatat ttacagtaag ctgaaactgt ataaatcttt
    12181 gaaaattctc atatatacct cttccccatt ttattccctc tactatagta ctaaagctat
    12241 catcagtcaa tattatatct gctgcatttt tagatacttc tgttcctgaa atacccattg
    12301 caattccaac atctgcttga ctaagagctg gggcatcatt tataccatca cctgttacag
    12361 ctacaacttc accacttttt tgaagtgctg aaactattct catttttgta tctggtttac
    12421 ttcttgcaac aatagatatt cctttgattt cttctctcaa ttctttatct gtcaatacat
    12481 ctatatatgt tgcttcaact gcctttttcc catcatttaa taatcctaat tcatttccaa
    12541 tagccgttgc tgtattgata ttatccccag taagcatttt tacatctact cctgcattaa
    12601 atgctttatc aatggactct ttgacccctt ctctcaatgg gtctactatt cccacaaatc
    12661 cactaaaaac taaattatct tctttcatat aacctctttg atttcctatt aaattcaatt
    12721 caccttctga agttattgca acttctgttt tagacttgct cattttttta taagcaaatc
    12781 ctaatgttct catagattta atttgtaatt tcttaatctc atctaaaata cttttttcta
    12841 tttttggagt tattggaaca atattttttc cttgttgtac ataagaacat ttttttaata
    12901 atacttctgg agcgcctttt gaaagtaata tatctgaatc ctcttgacga attaatgttg
    12961 acattttctt ttcttctgag ctaaatggta tttgtgatat caaataagtt tgttttctca
    13021 tttcattata gttcttatca ttgtgatata aaagtaatgc acactctgtt gcgcttccta
    13081 tatatttaaa tgaattgtct tctttttcta tatctgcagt tgaatttact atacagtttt
    13141 gttcaaaata actattactt tgatactctc ctccacttat atatttacca tcaacatatg
    13201 ctacctctac catcattttg ttttgtgtta aagtacctgt tttgtcagaa catattacag
    13261 aaacagaacc tatagtttca catgcttctt tctttgttac taatgcattt atctttgcca
    13321 tcttttgcat tgttatagct aaggtaatat ttatcatagt tggcaaacct tctggaacag
    13381 ttgcaactat taaagcaata caaaccatat atgcattttt agcaggttct aatgattgta
    13441 agaaagataa tacactgctt gtatctaaaa ttaacatacc ttttctctgc atctgtataa
    13501 tcataaacat acacaacaaa gtagctatta cacctgatat tcctgctatt ttagcaccca
    13561 acttccctaa tttaatttga agtggagttt gctgctcttc atttgataaa ttctgtgcta
    13621 tcttacccat ctctgtctta tcaccagtat gagtaacaac cattatacct ctaccatatg
    13681 ctacaagagt ccctccaaat accatattga cttgctttgc aggtattgca tcctgttcga
    13741 ttatttctgt tttagaataa actacttcca tatctaatac agcatcagca ttcttagcta
    13801 catcatcaga ttctcctgtt agcatatctt ctctaagttt caaatttata gactgtataa
    13861 gtcttccatc tgctggtatc atatctccag tttctatata tacaatatct cctggtacta
    13921 aatcattctt tgatatttta attatctttc catttctaag tacttttact tctatatttt
    13981 ctgttaattt tgacagggca tgagctgctt ttttagattt tccttcagtt attataccta
    14041 tagaaatccc aatgaaaata gcccctaaaa tacctaatgc atcatgtaat tctcctacaa
    14101 aagaacttat tactgctgct cctatgagaa ttaatatcat aggctctgtc aaactttctc
    14161 ccaactcgtc ccaaaatgtt cgaccttctt ttatagtaaa ttcatttaat ccatatctaa
    14221 gcttcctttc ttctacctca tcatcatcaa gtccaatttc tggatttgtt tttaagtatt
    14281 ttaatacttc tttggtaggt ttattataat acctcataac tttatccccc ttgttaatta
    14341 tctataagga attatatgct tatgatagct tgtatattac ttttcaaaaa aaataaggtg
    14401 ttgattaatc aacaccttat agtagttata tattattagg ttttacgtta taggcttcat
    14461 aagttgcggg ctatataaat caatacactt aatcttcaaa atatccttct ggatataatg
    14521 gaaacttaga tataagttct tgtgcttctg atttgcattc atctaatatt tctttattat
    14581 caatattttc tgctacctta ttcattatcc ctgctatctg tacaacttct ttttctttca
    14641 gacctctttg agatattgat gtaagaccta tacgaacacc actagttaca aatggtgatt
    14701 gtgggtcatc aggtataaca tttttattaa ctgttattcc tacatattca agggcttttt
    14761 caaattgttt tcctgtaaga ttcttacttc ttaagtcaac cattactata tgattatcag
    14821 tacctccact tacaatacta aaaccatgtg aaatcagttc agaagctaga gtctgagcat
    14881 ttactacaac ttgctccatt atctttttaa atttatctgt acttaaatat aatagtgaaa
    14941 aggcttttgc tgcaactgta ttaagatgta tagaacctaa tgttcctgga aaaacacctt
    15001 tatcgagttt ctttgcatgt tctgctttac aaaatacaat accacttctt ggtccacaga
    15061 aagtctttgt tgtagatgaa gaaacaaaat ctgcataagg tacaggacta ggtattactt
    15121 tcgctgcaac taatcctgca acatgtgcca tatctaccat aaagtaagct cctacttctt
    15181 ttgcgacctt tgatattctt tcataatcta ttaatcttgg atatgaactt gcccctgcaa
    15241 ttataagttt tggtctacat tcttttgcct ttgcttcaag agcatcataa tctatacgtc
    15301 cagtatttgg gtctacccca taaaattcat atttataaat atcatgtaaa aaattaactg
    15361 tgctaccatg tgtcaagtgt ccaccttggt caagacgcat acttaaaaca gtgtcatttg
    15421 gttttaatat tgatgaatat acacaatagt tagctgtaga acctgaatac ggttgtacgt
    15481 ttacatgttc tgctccaaat acttcttttg cacgttttat tgctaggatt tcaagtttat
    15541 ctgcttcttc agaacctgct tgaaaacgag cccctggata gccttcttct gttttatttg
    15601 taaatacaca tccacttaat tcaagtactt ctgttggtgc tgtactttca gaagctatca
    15661 tctcaatatt gtgttcttgt ctaaccaact catcagctac aatcttgtat aactctgggt
    15721 ctgaaatttt tgttgttttt aacataataa tcctcctctt aattaaagaa tatattttat
    15781 tagtttaatc ttcattgata aatttattat aacggtctat aaaacgtaag atattcctcc
    15841 accaggtcct aatggtatgc caatacccat gaatagtaac aatattatta tttctattaa
    15901 tgtaaatacc actgagtatg gaatcatatt cgatatgaca gtacctatac ctatatcttt
    15961 atcatatctt ctggcaaagg ccagtataac tggaaaatac ggaaaaagtg gacttattgg
    16021 atttgttgaa gcatctccaa tacgataagc tatttgagtt aatgctggat taaagtctag
    16081 tagcataaac atgggaacaa atataggtgc taaaatcgcc cattttgcag aagcacttcc
    16141 tacaaataaa ttgataaatc cagatagcaa tataaatcct attatcaatc catagctagg
    16201 tattcccgac tctgctagta attttgctcc tgttattgat aaaattgttc ctaagttact
    16261 aagattaaat aaattaataa actgggatgc tataaatgct aggactatat atccacccat
    16321 atcactcata ctactaccca tcatagatac taaatctttg tcacttttta tcttcttact
    16381 tacttttcca tacactaatc ctggagttaa aaatataata gtgattatag gaactatacc
    16441 tttcattaaa ggtgcattac tagatagtat agaaccagtc tctggatctt tcataaatgc
    16501 attgtcacct atacataagg caactatcac tactactaca actaacaatg ataataaagc
    16561 gtattttata cccttatttt caagttcact caattcctgt atatccttat catttgttga
    16621 atcattatat tttgtaaaac gaggagctat aaatttttca gttacaaatg ctcctgcaaa
    16681 agttaaaact atcgctgata taaaagcaaa ataaagattc attgctggat tgccttggta
    16741 agatgggtcc atcatttgag ctgctggtat tgtaaatcca gctaacaata catctgtagt
    16801 actaagcata acatttgctg caaatccccc tgcaacacct gcaaaagctg caaacatacc
    16861 tacaagagga tgtcttccaa ttcctaaaaa cacaagtgct gcaagtggtg gtaatattat
    16921 aaatcctaca tctgctgctg tatgtgacaa catacctgca aatacgattg ctacagttac
    16981 aagtttttgt ggaactttac ttataccctt tttcattaac acttccataa atccagattt
    17041 ctcagcaaca cctgctccaa gcattgtaac cagtactaat cctaaaggag caaatgactg
    17101 aaagtttgta actatgctac ctaaatatga ttgaatctgc tctttctcta gtagatttac
    17161 tacctttaca acttcttttg tagaaggatg tatttcttct actcctaaat tagatataac
    17221 tgctgacaat attacaacaa ttatacatag taataaaaat atggttactg ggtctggaag
    17281 tttatttcct gctctctcta caacatttag tgccctcatc aataatccac tatctttgtt
    17341 tttttcctgt gaattaattt tcctcaaact aataccccct cataaacttt atctccagaa
    17401 tgctctatct taaattttat tttttaattt atccatataa tttataacag actgacatag
    17461 tacatttgta cctactaata tagaattttt atcagcttta aatttaggat tatgccactg
    17521 atatatacaa tcttgttctt tgttgcctac ccctaaccag taaaaaaatc ctggggcttt
    17581 ttccatgaat atagaaaaat cttctcctcc tgtagaaggt attggatcaa ctatagcatc
    17641 ttctgaatac aaatcacaca cagacttaca agctattgcg tacatctctt tactatttat
    17701 aacagctggt aaatcaaata aataatctag ctttgcagtt gcttgatatc cacgagctat
    17761 ctcacaaact atattttcta aattttctga tattttcttt cgtacttcat tactaaagct
    17821 tctacatgtg cctgtcatat ctaccttttc tgaaattaca ttatttgcag tccctccatt
    17881 tatagtacct attgatataa cagctgattc taatggactt atgtttctgc ttactatggt
    17941 ttgaataccc attataattg cacttgatgc tactataggg tctattgttc tgtttggtat
    18001 acctccgtga cctccaaatc catttacttc tatcttaata gtgtctactg ctgccattaa
    18061 cccacctaac ttaacaccta tttttccaca aggtatatct ggatgattat gaagtccaaa
    18121 tatagcatca acttttggat tttccattac tcctcgttcc actagcatct tcgcaccttt
    18181 attgacttct tcagctggct gaaatataaa ctttacattt ccatgaagac tatctctaat
    18241 atgagacaat acatatgcac atcctacaag gcaagatgtg tgtatatcat gcccacatgc
    18301 atgcattata ccatcatatc tggatttata ctcaatatca acctcttcat atattggcaa
    18361 tgcatcaata tctcctctta gcgctactgt gggaccatca tacttccctt ttaataatcc
    18421 aacaactcct gtttccattc ctaagtctaa tatttctatg tctaaactta ctaataaatc
    18481 ttttatagct tttgtagtcc taaattcttt aaaacttaat tcggggtgca tatgaatatc
    18541 tgtaaaaaaa tctaaaatat cttctttata cttttctgag attaaaataa gttcatgcat
    18601 actaacacct ctccaaaatt ttattttttt at
//
LOCUS       DAEPXF010000072        18578 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain HB27
            SAMN13376389-rid12479423.denovo.076, whole genome shotgun sequence.
ACCESSION   DAEPXF010000072 DAEPXF010000000
VERSION     DAEPXF010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13376389
            Sequence Read Archive: SRR10518432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 18578)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18578)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001069624.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001069624.1
            Genome Coverage       :: 315x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/15/2021 01:54:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,165
            CDSs (total)                      :: 4,076
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 89
            rRNAs                             :: 10, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 10 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 19 of 69
            Pseudo Genes (incomplete)         :: 44 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 10 of 69
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18578
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13376389-rid12479423.denovo.076"
                     /strain="HB27"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="China: Hebei"
                     /collection_date="2017"
     gene            100..1701
                     /locus_tag="KRL67_002094"
     CDS             100..1701
                     /locus_tag="KRL67_002094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp11"
                     /protein_id="HBH1522971.1"
                     /translation="MKMNKKILSLGLAVSLILVNFKSVNASSVVEKIYGKDRYETAAK
                     IADKQTYETVILVNTEKSLADGLSASGLSGATKAPILFTQQNKIPADTNRCLKNIKKA
                     YIIGTEDTISKSVEKELDSKNIEVKRIGGEDRLKTSYLIAKEIATIKKVDKVLLTNAY
                     SGEADAMSVSSVATRDGAPIILTDGKSVPFDVKNIQSYCIGSEEIMSNPLVKNTNSVR
                     IEGTDRFETNKNVIDYFFNSADGFYVSDGYQLVDAIAAAPLTKNSPMVLVNDGSDKIV
                     LEGAKNITSVGEINEKVIQQCINASKSNGQPPTITVGSTEVYKGEKFDTGKLNIVAKD
                     NTGKVLPIEVDGFIDTNRVGTYILTLKATDEWGKSAGKRVEIKVLDDKSHDYNSPEFK
                     KMVSTEMYNLINSYRKEKGKEPLVVSSRLEGMANAWSKYMMDKKVFAHYIDGKNAPQV
                     FSEFGMRSEENIAYIYIDSKNVQTTQDAKDLAKAIFEVWKKSPEYNANMLSDEFYSTG
                     FGLYILSDGQVHATQEFLNGNEGSL"
     gene            1920..3506
                     /locus_tag="KRL67_002095"
     CDS             1920..3506
                     /locus_tag="KRL67_002095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp12"
                     /protein_id="HBH1522972.1"
                     /translation="MKVNKRVLSIGLTISLIMAGAPNINALSSIEKIQGKDRYETAAK
                     IAQKQTYENVVLVNTDNTLADGLSASGLAGTVKAPILLSQKNSIPSDTEKMLKDVKKA
                     YIIGTEDSIGKSVENELKQKGIEVKRIGGNDRIETSYLIAKEIASIKPIDKVFITNGY
                     TGEADAMSASSVASRDGAPIILTNGKNVPFEKKEGVQCYALGSEEIISNDLVKKTNAV
                     RLAGEDRFETNKKVIKHFYSSAKEFYLSKGYQLVDAVAGSSIAKNAPIVLVDGNSDKS
                     VLRSADKITALGGIDEKTLEQCLSASSLDASAPTITVGNLNIYQGDKFDISKLNIVAK
                     DSNGNDLTPELIGNINTDKVGKYKVTIKATDIGGKTTSINVEVNVLEYKTNDMNSSEF
                     KRMVSSEIYSLVNSYRKEKGKEALKVSEDLQGMANYWSKYMADKGEFAHVINGKNAAE
                     VFSGGMRSEENIAFVPLTTKSTYTTKDAREVANVIFTVWKKSDKYNENMLNSNFAYTG
                     FGLYILPDGQVYATQEFLNK"
     gene            3710..5854
                     /gene="slpA"
                     /locus_tag="KRL67_002096"
     CDS             3710..5854
                     /gene="slpA"
                     /locus_tag="KRL67_002096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-layer protein SlpA"
                     /protein_id="HBH1522973.1"
                     /translation="MNKKNLAMAMAAVTVVGSAAPIFADSTTPGYTVVKNDWKKAVKQ
                     LQDGLKNKTISTIKVSFNGNSVGEVTPASSGAKKADRDAAAEKLYNLVNTQLDKLGDG
                     DYVDFEVTYNLATQIITKAEAEAVLTKLQQYNDKVLINSATDTVKGMVSDTQVDSKNV
                     AANPLKVSDMYTIPSAITGSDDSGYSIAKPTEKTTSLLYGTVGDATAGKAITVDTASN
                     EAFAGNGKVIDYNKSFKATVQGDGTVKTSGVVLKDASDMAATGTIKVRVTSAKEESID
                     VDSSSYISAENLAKKYVFNPKEVSEAYNAIVALQNDGIESDLVQLVNGKYQVIFYPEG
                     KRLETKSADIIADADSPAKITIKANKLKDLKDYVDDLKTYNNTYSNVVTVAGEDRIET
                     AIELSSKYYNSDDKNAITDDAVNNIVLVGSTSIVDGLVASPLASEKTAPLLLTSKDKL
                     DSSVKSEIKRVMNLKSDTGINTSKKVYLAGGVNSISKDVENELKNMGLKVTRLSGEDR
                     YETSLAIADEIGLDNDKAFVVGGTGLADAMSIAPVASQLKDGDATPIVVVDGKAKEIS
                     DDAKSFLGTSDVDIIGGKNSVSKEIEESIDSATGKTPDRISGDDRQATNAEVLKEDDY
                     FKDGEVVNYFVAKDGSTKEDQLVDALAAAPIAGRFKESPAPIILATDTLSSDQNVAVS
                     KAVPKDGGTNLVQVGKGIASSVINKMKDLLDM"
     gene            6100..8445
                     /gene="secA"
                     /locus_tag="KRL67_002097"
     CDS             6100..8445
                     /gene="secA"
                     /locus_tag="KRL67_002097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HBH1522974.1"
                     /translation="MSVIDSILDKADEQEIKKLNVIVDKIDALEDSMKNLSDEELKDM
                     TAIFKNRLKKGETLDDILPEAFAVVREVSKRKLGMRQYRVQLIGGIVIHQGKIAEMKT
                     GEGKTLVEVAPVYLNALTGKGVHVITVNDYLAERDKELMSPVYESLGMTVGVIISNQD
                     PNIRKQQYKCDITYGTNSEFGFDYLRDNMVPDLSYKVQRELNFAIVDEVDSILIDEAR
                     TPLIIAGDGDEDLKLYELANSFIKTVKEEDFEMDRKDKTIALTASGISKAESFFGITN
                     LTDIKNIELYHHINQALRGHKLMEKDVDYVISNGEVMIVDEFTGRVMDGRRYTDGLHQ
                     AIEAKEGVEIKNESKTMATVTYQNFFRLYEKLSGMTGTAKTEEGEFESIYKLNVVQIP
                     TNKPVIRADLHDKVFKTEEEKYSAVVEEIIRIHKTRQPILVGTVSVEKSEKLSRMLKK
                     QGIKHQVLNAKQHDKEAEIISKAGKLDAITIATNMAGRGTDISLGAGDKEEEQEVKDL
                     GGLYVIGTERHESRRIDNQLRGRSGRQGDPGTSRFFVSLEDDVIKLYGGKTIEKLMKR
                     TSSKENTAIESKALTRAIERAQKGVEGKNFEIRKNVLKYDDTINEQRKVIYNERNKVL
                     NDEDIQEDIQKMVKDIIQEAGETYLIGRKRDYYGYFKHLYSTFMPADTLLIPGVDKKS
                     VQEIIDSTYEISKRVYDLKKMMIGIDKVAELEKTVLLKVVDQYWIDHIDAMEQLKQYI
                     GLKSYAQKDPFKEYALEGYDMFEALNKNIREATVQYLYKFN"
     gene            8860..10731
                     /locus_tag="KRL67_002098"
     CDS             8860..10731
                     /locus_tag="KRL67_002098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp2"
                     /protein_id="HBH1522975.1"
                     /translation="MNKKNLSVIMATTMISTSVAPVFAAETTQVKKETITKKEATELV
                     SKVRDLMSQKYTGGSQVGQPIYEIKVGETLSKLKIITNIDELEKLVNALGENKELIVT
                     ITDKGHITNSANEVVAEATEKYENSADLSAEANSITEKSKTETNGIYKVADVKASYDS
                     AKDKLVITLRDKTETVTSRTIYVGIGDEKVDLTANPVDSTGTNLDPSAEGFRVNKIDK
                     LGVAGAKNIDDVQLAEITIKNSDLNTVSPQDLYDGYRLTVKGNMVANGTSKSISDISA
                     KDSETGKYKFTIKYTDASGKATELTVESTNEKDLKDAKAALEGNSKVKLIAGDDRYVT
                     AVAIAKQTKYTDNIVIVNSNKLVDGLAATPLAQSKKAPILLASDNEIPKVTLDYIKDI
                     IKKSPSAKIYIVGGESAVSNTAKKQLESVTKNVERLAGDDRHTTSVAVAKAMGSFKDA
                     FVVGAKGEADAMSIAAKAAELKAPIIVNGWNDLSADAIKLMDGKEIGIVGGSNNVSSQ
                     IENQLADIDKDRKVQRVEGETRHDTNAKVIETYYGKLDKLYIAKDGYGNNGMLVDALA
                     AGPLAAGKGPILLAKTDITDSQKNALSKKLNLGAEVTQIGNGVELTVIQKIAKILGW"
     gene            10838..11545
                     /locus_tag="KRL67_002099"
     CDS             10838..11545
                     /locus_tag="KRL67_002099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HBH1522976.1"
                     /translation="MVDATLFFTPHQDDETLSMGSAIIEHVEKSDTHVILCTDGSKSI
                     IRKVLDDGGSCSYHIKDVHKYSLSESEFSKDRDEEFKDSCEAMGVKESNIHIENNRAH
                     DGELSKEKAREIILKYLEEYPDAKVKTVTPFKASGIHEDHRALGEAALELYREGKIKD
                     LRFYVEPYDYKDFKKVNPNVEVWKVLPSQEEKLLSAMNAYKKWNPESGHYAIGYHSVK
                     SHFDELATNKIQYVHAP"
     gene            11578..13410
                     /locus_tag="KRL67_002100"
     CDS             11578..13410
                     /locus_tag="KRL67_002100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp66"
                     /protein_id="HBH1522977.1"
                     /translation="MKISKKIVSLLTMTFLTVTLYGNTSNASTKDTLTGSGRWETAIK
                     ISQAGWKKSENAVLVNDNSIADALSATPFAKAKDAPILLTQSNKLDSRTKAELKRLGV
                     KNVYLIGGSIALSSEIEKQLNAENINFERISGNSRYDTSLKLAEKLDREKSISKIVVV
                     NGEKGLADAVSVGAIAAQENMPIILSDSDNGTEVADNFIDSKDIEKSYVIGGTYSISN
                     SVERSLPNATRIAGSSRSETNAKIIEEFYKDTDIKNIYVTKDGTKNKNDLIDSLAVGV
                     LAAKNSSPIVLAGNKFDTTQKDVLNTKTIDKVTQIGGLGNENVVEDILDIQEETKYTV
                     ETIDELNAAIKRADANDIIKFKPEKEKTINNSFSIETKKTVTIELDGRYRQTITLDIP
                     NGKFNNYAEIEGGVKLKNIKNESLVNKGSIQDLDIYDENGCKIENESSGEIWFVTIVE
                     EANDVYIVNSGDITKISNNSSGTIIRNSGNIDTVTGKKEPAISGNKPKVNDTEKETKA
                     ARGLNPRVEACSVPKKDYVMITIPNSPKDSRYKIYYRVVYNKPYAMDVGDKINIGEWT
                     VAPTDEEPFLEKAKNGCYVEAVEVNTSTKEVSRWGRTNVTDDGF"
     gene            complement(13457..13858)
                     /locus_tag="KRL67_002101"
     CDS             complement(13457..13858)
                     /locus_tag="KRL67_002101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HBH1522978.1"
                     /translation="MILKKYKETILYLFFGAFTTLVNIVSYLFFTRVILFNFMVANAL
                     AWILAVLFAYVTNKFFVFESKRIEIKFLFKEFLSFVSFRLLSGVVEMLIMYVMIDLLF
                     VNDVIVKVFTNIVVIVLNYLFSKMIIFKNKK"
     gene            14086..16497
                     /locus_tag="KRL67_002102"
     CDS             14086..16497
                     /locus_tag="KRL67_002102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding cysteine protease Cwp84"
                     /protein_id="HBH1522979.1"
                     /translation="MRKYKSKKLSKLLALLTVCFLIVSTIPVSAENHKTLDGVETAEY
                     SESYLQYLEDVKSGDTAKYNGVIPFPHEMEGTTLRNKGRSSLPSAYKSSVAYNPMNLG
                     LTTPAKNQGSLNTCWSFSGMSTLEAYLKLKGYGTYDLSEEHLRWWATGGKYGWNLDDM
                     SGSSNVTAIGYLTAWAGPKLEKDIPYNLKSEAQGATKPSNMDTAPTQFNVTDVVRLNK
                     DKETVKNAIMQYGSVTSGYAHYSTYFNKDETAYNCTNKRAPLNHAVAIVGWDDNYSKD
                     NFASDVKPESNGAWLVKSSWGEFNSMKGFFWISYEDKTLLTDTDNYAMKSVSKPDSDK
                     KMYQLEYAGLSKIMSNKVTAANVFDFSRDSEKLDSVMFETDSVGAKYEVYYAPVVNGV
                     PQNNSMTKLASGTVSYSGYINVPTNSYSLPKGKGAIVVVIDNTANPNREKSTLAYETD
                     IDGYYLYEAKANLGESYILQNNKFEDINTYSEFSPCNFVIKAITKTSSGQATSGESLT
                     GADRYETAVKVSQKGWTSSQNAVLVNGDAIVDALTATPFTAAIDSPILLTGKDNLDSK
                     TKAELQRLGTKKVYLIGGENSLSKNVQTQLSNMGISVERISGSDRYKTSISLAQKLNS
                     IKSVSQVAVANGVNGLADAISVGAAAADNNMPIILTNEKSELQGADEFLNSSKITKSY
                     IIGGTATLSSNLESKLSNPTRLAGSNRNETNAKIIDKFYPSSDLKYAFVVKDGSKSQG
                     DLIDGLAVGALGAKTDSPVVLVGNKLDESQKNVLKSKKIETPIRVGGNGNESAFNELN
                     TLLGK"
     gene            16794..18371
                     /locus_tag="KRL67_002103"
     CDS             16794..18371
                     /locus_tag="KRL67_002103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp5"
                     /protein_id="HBH1522980.1"
                     /translation="MKAPKTILTILTIALTLSSISIIPSYALTEEKLIGNGRYETAVK
                     ISQKAYSSSSNVVLVNDNSLADALSATPFAKAKGAPILLTESDKLDDRTEKEIKRLGA
                     KDIYLIGGTAVLNKDIENKLKGNGLNVERINGKDRYETSLILANKLKDIKDIKEVAVV
                     NGEKGLSDAVSVGAPAAQNKMPIILSSPKDGVEAFDKFIRDEKVIKAYVIGGTTSVSR
                     AVEKSLPNAERLSGKDRNETNAKVIEKFYTDTNLSNLYVTKDGSKNENQLIDSLAVGV
                     LAAKNESPVVLVGNKLNTKQRDILSTKKLNTITQVGGNGNEEAFDEIKSLQEKTVFEA
                     KTVEELTDMINIASPNDIINFKPKENTVNEAFRMVTNKPITVNIKGDCSKTITVDMPN
                     GEVNNYATLVNVIVRNIGEGGFNNHDTITILSVRDKNGRVIENTRNSDIDTLMILASA
                     NDTKLINDGYIGKLIDNSSNSDITNNGTIDKKVNQVEDLEAKVDSIEKAIDSISQKVN
                     KIQDILDKLGFLKKFLS"
BASE COUNT         7693 a         2095 c         3446 g         5344 t
ORIGIN      
        1 ttcatatcat atatactcta taatatatgg aaaataaaga gaaaataaat ggaaaataat
       61 tacaatataa acataaaata taaaaatcat cagggggata tgaaaatgaa caaaaaaata
      121 ttatcattag gtctagcagt atcattaatc ttagtaaact tcaaaagtgt aaatgcatca
      181 tcagtagtag aaaaaatata cggtaaagat agatatgaaa cagcagcaaa gatagctgat
      241 aaacagactt atgaaacagt gattttagta aatacagaaa aatcacttgc agatggatta
      301 agtgcaagtg gattatcggg ggctacaaaa gctccaatac tatttacaca acaaaataag
      361 ataccagcag acacaaatag atgtctaaaa aatatcaaaa aagcatatat aataggtaca
      421 gaagatacta taagtaaatc agtagaaaaa gaactagatt ctaaaaatat agaagtcaaa
      481 agaattggtg gagaagatag actaaaaaca agctatttaa tagctaaaga aatagcaact
      541 ataaaaaaag tagataaagt actattaact aatgcatata gtggagaagc agatgcaatg
      601 agtgtatcat cagtagctac tagagatgga gctccaatca tacttacaga tggaaagagt
      661 gtgccttttg atgtaaaaaa tattcaatca tattgtatag gttcagagga gataatgagt
      721 aatcctttag ttaaaaatac aaactcagta agaatagaag gaactgaccg atttgaaact
      781 aacaaaaacg taatagacta cttttttaat agtgcagatg gattctatgt atcagatgga
      841 taccaattag tagatgcaat agcagccgca ccacttacta aaaactctcc aatggtacta
      901 gtaaatgatg gaagcgataa gattgtatta gaaggagcta agaatataac ctctgtaggt
      961 gaaataaatg agaaggtaat acaacagtgt ataaatgctt caaagtcaaa tggacaaccc
     1021 ccaacaatta cagttgggag tacagaggta tataagggtg aaaagtttga cactggcaag
     1081 ttaaatatag tggctaaaga taacactggg aaggtattac caatagaagt tgatgggttt
     1141 atagatacaa atagagtagg tacatacata ttgacattaa aagctacaga cgaatgggga
     1201 aaaagcgcag gaaaaagagt agaaataaaa gtgttggatg ataaatctca tgattacaac
     1261 agcccagaat ttaaaaaaat ggtatctact gaaatgtata acttaatcaa ttcctataga
     1321 aaagaaaaag gcaaagagcc tttggtagta tctagtaggc tagaaggtat ggcaaatgca
     1381 tggtctaagt atatgatgga taaaaaagta tttgcacatt atatagatgg aaaaaatgct
     1441 ccacaagtat tttctgagtt tggaatgaga agtgaggaaa atatagcata tatttatata
     1501 gattctaaga acgttcaaac gacacaagat gcaaaagact tggcaaaggc tatatttgaa
     1561 gtgtggaaga aatctccaga gtacaatgca aacatgctta gtgatgagtt ctatagtact
     1621 ggatttgggc tttatatatt atctgatggt caagtgcatg ctactcaaga gtttttaaat
     1681 ggcaatgaag gtagcttgta aaaaattcca taaatcttgt ttttttgtta atttactgat
     1741 attataaaat gaaaatcaca tctaaagtac tttttgtaat ctgttttgct attgctttac
     1801 aggttatggt attttaggtg ttttttgttt tagtagttta taaattttga gggaatcact
     1861 actgaggatg taatttatga ttagctgaat tatataaaaa aattagtagg aggagtttga
     1921 tgaaggtaaa taagagagta ttatcaatag gattgacaat atcattaata atggcaggag
     1981 caccaaatat caatgcactt tcaagtatag aaaagataca ggggaaagat agatatgaga
     2041 cagctgccaa aattgcacag aaacaaacat atgaaaatgt agtattagta aatacagata
     2101 atacactagc tgatggatta agtgcaagtg gattagcagg aacagttaaa gcacctatat
     2161 tattatcaca aaaaaacagt ataccatcag acacagaaaa aatgttaaaa gatgtaaaga
     2221 aggcttatat aataggaact gaagattcaa taggaaaatc agttgaaaat gaattaaagc
     2281 aaaaagggat agaagttaaa agaataggtg gtaatgatag aattgaaaca agttatctta
     2341 tagcaaaaga aatagcttca ataaaaccta tagataaggt ttttataact aatggttata
     2401 caggtgaagc agatgctatg agtgcgtcat cagtagcatc aagagatgga gcacctataa
     2461 ttttaactaa tggtaaaaat gttccatttg agaaaaaaga aggtgtgcaa tgttatgctc
     2521 ttggttcaga ggagataata agtaatgatt tagttaaaaa aactaatgca gttagattag
     2581 ctggagaaga tagatttgag actaataaaa aagttataaa acatttttat agttcagcaa
     2641 aagaatttta tctttctaaa ggttatcaat tagtagatgc agttgctggt tcttctatag
     2701 caaaaaatgc acctattgta ttggttgatg gaaatagtga taagtctgtt cttagaagtg
     2761 ctgataaaat cacagcacta ggaggaatag atgaaaagac gctagaacaa tgtttaagtg
     2821 catcatcact ggatgcaagt gcaccaacaa taactgttgg aaatttaaat atttatcaag
     2881 gtgataagtt tgatataagt aaacttaata tagtagcaaa ggatagcaat ggtaacgatt
     2941 tgacaccaga gcttatagga aatattaata ctgataaggt gggtaaatac aaagttacta
     3001 taaaagctac tgatattggt ggaaagacta cctcaataaa tgtagaagtg aatgtacttg
     3061 aatataagac taacgatatg aatagtagtg agtttaaaag aatggtttct agtgaaatat
     3121 atagtctggt aaattcatat agaaaagaaa aaggtaaaga ggcattaaaa gtttctgaag
     3181 atttacaagg aatggcaaat tattggtcta agtatatggc tgataaaggt gaatttgcgc
     3241 atgtaataaa tggtaaaaat gctgctgaag tattttctgg tgggatgaga agtgaagaaa
     3301 atatagcttt tgtaccactt actacaaaga gtacttatac aacaaaagat gctagggaag
     3361 tggcaaatgt catatttaca gtttggaaga agtctgataa gtataatgaa aatatgttga
     3421 atagcaactt tgcatatact ggatttggac tatacatatt accagacgga caagtgtatg
     3481 ctactcaaga atttttaaat aaataaaatt aaagttttat ttcattagat tattatggta
     3541 taaaaactta tatatttgga gaaaataata atttaaaaaa atagcttgca aaaagaataa
     3601 aaatggatta ttatagagat gtgagaaata ttaggaatat atggatgatt attctatgta
     3661 cataataaag agatgtaatt ttaatataat gttgggagga atttaagaaa tgaataagaa
     3721 aaacttagca atggctatgg cagcagttac tgttgtgggt tctgcagcgc caatatttgc
     3781 agatagtact acgccaggtt atactgtagt gaaaaatgat tggaaaaaag cagtaaaaca
     3841 attacaagat gggttgaaaa ataaaactat atcaacaata aaggtgtctt ttaatggaaa
     3901 ctctgttgga gaagttacac cagccagttc tggagcaaaa aaagcagata gagatgctgc
     3961 agctgaaaag ttatataatt tagtaaatac acaattagat aaactaggtg atggagatta
     4021 cgttgacttt gaagtaactt ataatttagc tactcaaata attacaaaag cagaagcaga
     4081 ggcagttctt acaaaattac aacaatataa tgataaagta cttataaatt ctgcaacaga
     4141 tacagtaaaa ggtatggtat ctgatacaca agttgatagc aaaaatgttg cagctaaccc
     4201 acttaaagtt agtgatatgt atacaatacc atctgctatt actggaagtg atgattctgg
     4261 gtatagtatt gctaaaccaa cagaaaagac tacaagttta ttgtatggta cggttggtga
     4321 tgcaactgca ggtaaagcaa taacagtaga tacagcttca aatgaagctt ttgctggaaa
     4381 tggaaaggtt attgactaca ataaatcatt caaagcaact gtacaaggag atggaacagt
     4441 taagacaagc ggggttgtac ttaaagatgc aagtgatatg gctgcaacag gtactataaa
     4501 agttagagtt acaagtgcaa aagaagaatc tattgatgtg gattcaagtt catatattag
     4561 tgctgaaaat ttagctaaaa aatatgtatt taatcctaaa gaggtttctg aagcttataa
     4621 tgcaatagtt gcattacaaa atgatggaat agaatctgat ttagtacaat tagttaatgg
     4681 aaaatatcaa gttattttct atccagaagg aaaaagatta gaaactaaat ctgcagatat
     4741 aatagctgat gcagatagtc cagctaaaat aactataaaa gctaataaat taaaagattt
     4801 aaaagattat gtagatgatt taaaaacata caataatact tactcaaatg ttgtaacagt
     4861 agcaggagaa gatagaatag aaactgctat agaattaagt agtaaatatt ataattctga
     4921 tgataaaaat gcaataactg atgatgcagt taataatata gtattagttg gatctacatc
     4981 tatagttgat ggtcttgttg catcaccatt agcttcagaa aaaacagctc cattattatt
     5041 aacttcaaaa gataaattag attcatcagt aaaatctgag ataaaaagag ttatgaactt
     5101 aaagagtgat actggtataa atacttctaa aaaagtttat ttagctggtg gagttaattc
     5161 tatatctaaa gatgtagaaa atgaattgaa aaatatgggc cttaaagtta ctagattatc
     5221 aggagaagac agatacgaaa cttctttagc aatagctgat gaaataggtc ttgataatga
     5281 taaagcattt gtagttggtg gtactggatt agcagatgct atgagtatag ctccagttgc
     5341 ttctcaactt aaagatggag atgctactcc aatagtagtt gtagatggaa aagcaaaaga
     5401 aataagtgat gatgctaaga gtttcttagg aacttctgat gttgatataa taggtggaaa
     5461 aaatagcgta tctaaagaga ttgaagagtc aatagatagt gcaactggaa aaactccaga
     5521 tagaataagt ggagatgaca gacaagcaac taatgctgaa gttttaaaag aagatgatta
     5581 tttcaaagat ggtgaagttg tgaattactt tgttgcaaaa gatggttcta ctaaagaaga
     5641 tcaattagta gatgcattag cagcagcacc aatagcaggt agatttaagg agtctccagc
     5701 tccaatcata ctagctactg atactttatc ttctgaccaa aatgtagctg taagtaaagc
     5761 agttcctaaa gatggtggaa ctaacttagt tcaagtaggt aaaggtatag cttcttcagt
     5821 tataaacaaa atgaaagatt tattagatat gtaatataag ttttaataaa accttaaata
     5881 gaaaaaggct tctctcatga gaagtctttt tctatttaaa ataaatataa aataaaatag
     5941 aggctattta tagcctctat tttatgtgag aaatacttaa ataaaaagat gtttaattta
     6001 tttaagtaaa atcattatac tataaatcaa aataattgtc tactattatg gatttttaat
     6061 gtataatagt aattggacaa tagaaaagga ggtacttata tgtcagttat agattcgata
     6121 cttgacaaag cagatgagca agaaataaag aaattgaatg ttatagtaga taaaatagat
     6181 gcattagaag atagtatgaa aaatctatca gatgaagaac taaaagacat gacagctata
     6241 tttaaaaata gacttaaaaa aggtgaaacc ttagatgata tacttccaga agcatttgct
     6301 gtagtaagag aagtatcaaa aagaaaattg ggaatgcgtc aatatagagt tcaattaatt
     6361 ggtggtatag taatacatca aggaaagata gcagagatga aaacaggtga aggtaaaact
     6421 ctagttgaag tagcaccagt atatttaaat gcccttacag gtaagggtgt acatgtaatc
     6481 acagtaaatg attacctggc agaacgtgat aaggagctta tgagcccagt ttatgaatct
     6541 cttggtatga cagtaggagt aattatatct aaccaagacc caaatataag aaaacaacaa
     6601 tataaatgtg atataactta tggtacaaat agtgaatttg gatttgatta tttaagagat
     6661 aatatggtgc cagatttatc ttataaagta caaagagaac taaactttgc catagtagat
     6721 gaggtagact caatattaat agatgaagct agaactccac ttattattgc aggagatggt
     6781 gatgaagatt taaagcttta tgagttagca aatagcttta taaaaactgt aaaagaagaa
     6841 gactttgaga tggacagaaa agataaaact atagcattaa cagcaagtgg tataagtaaa
     6901 gctgagtcat tttttggtat aacaaacctt actgatataa agaacataga attatatcat
     6961 catataaatc aagctttaag aggtcataag cttatggaaa aagatgttga ctatgttatc
     7021 tcaaatggag aagtaatgat agttgacgaa tttacaggaa gagtaatgga tggaagaaga
     7081 tatacagatg gacttcacca agctatagaa gccaaagaag gtgttgagat aaagaatgaa
     7141 tctaagacta tggctactgt tacttatcaa aatttcttca ggctatatga aaaactttct
     7201 ggaatgactg gtactgcaaa gacagaagaa ggggaatttg agtcaatcta taaattaaat
     7261 gttgtccaaa taccaactaa taaaccagtg attagagcag atttacatga taaggtattt
     7321 aaaacagaag aagaaaagta tagtgctgtt gtagaagaaa taataaggat acataagact
     7381 agacagccaa tacttgtagg aacagtttct gttgaaaaat ctgagaagct gtctagaatg
     7441 cttaaaaaac aaggtattaa gcatcaagtg ttaaatgcta aacaacatga taaagaggca
     7501 gagataattt ctaaagctgg taaattagat gctataacga ttgctacaaa tatggcaggt
     7561 agaggaaccg atatttctct aggtgcagga gataaagaag aagaacaaga ggttaaagat
     7621 ttaggtggac tttatgttat aggaacagaa agacacgaat caagaagaat tgataaccag
     7681 cttagagggc gttctggtcg tcaaggagat ccaggtacat caagattctt tgtaagtctt
     7741 gaagatgatg taataaagct ttatggtgga aaaactatag agaaacttat gaagagaaca
     7801 agttcaaagg aaaatactgc tattgaaagt aaagcactta caagggctat agaaagagca
     7861 caaaaaggtg tagaaggtaa aaattttgaa ataagaaaaa atgttcttaa atatgatgat
     7921 actattaatg agcaaagaaa agttatatat aatgaaagaa ataaagtgtt aaatgatgaa
     7981 gatatacaag aagatattca aaaaatggtt aaagacatca tacaagaagc aggagaaact
     8041 tatttaattg gaagaaaaag agattactat ggatatttta aacatttata tagtacattt
     8101 atgccagcag atacattact aatacctggt gtagataaaa aaagtgtcca agagataatt
     8161 gatagcacat atgaaatttc aaaaagagtt tatgacttga aaaagatgat gattggtatt
     8221 gataaggttg cggagttaga aaaaacagta cttttaaaag tggttgatca atactggata
     8281 gatcatatag atgctatgga acagttaaaa cagtatatag gtcttaaatc ttatgctcaa
     8341 aaagacccat ttaaggaata tgctttagaa ggatatgaca tgtttgaagc tttaaataaa
     8401 aatataaggg aagcaacagt gcaatactta tataaattta actaaaaaag ttcatgtaat
     8461 ttttattaaa tgaaaaataa agtattttga ttctttatat tgtaattaca attaaataat
     8521 taaggaatac aaatgaaaaa gaagctgaaa atcagcttct ttttctgtgt gagaaatacg
     8581 gaaaaaagag atgcttgaaa tttctttctt ccatttaata ttatactata aagaagatga
     8641 aaagtctatt agttataaac tggattatta aaaaaaatta caattttgtt acttttttgt
     8701 atacataaat attaaaaaaa taaaatataa gaaaaagttt gtatcttttg gttaattatt
     8761 acaataagtc tcatttattg aaataatatc aaatatatat tataattggt aaataaggaa
     8821 aaaataataa aaatttgaat tttttagggg gaaaatacca tgaataaaaa aaatctttct
     8881 gtaattatgg ctactacaat gataagtaca tcagtagctc cagtttttgc tgcagaaact
     8941 acacaggtaa aaaaagaaac aataactaag aaagaagcta cagaactagt ttcgaaagtt
     9001 agagatttaa tgtctcaaaa gtatacagga ggttctcaag ttggacaacc aatatatgaa
     9061 ataaaagttg gtgagacttt atcaaaatta aaaataataa ctaatataga tgaattagag
     9121 aaattagtaa atgctttggg agaaaataaa gagcttattg taactataac agataaaggg
     9181 catataacaa atagtgcaaa tgaagtagtt gcagaagcaa ctgaaaaata tgaaaattca
     9241 gcagaccttt ccgctgaagc taactctata acagaaaaat ctaaaactga aactaatgga
     9301 atttataaag ttgcagatgt aaaagcttca tatgatagtg ctaaagataa gttagttata
     9361 actttaagag ataaaacaga aacagtaact tctagaacta tatatgtagg tattggtgat
     9421 gaaaaagttg atttaacagc aaatccagtt gattcaactg gaacaaactt agacccttct
     9481 gcagaaggat ttagagtaaa taaaatcgat aaattaggtg tagcaggagc taaaaatatt
     9541 gatgatgttc aattggctga aataactata aaaaatagtg acctaaatac agtttcacca
     9601 caagatttat atgatggata tagattgact gttaaaggta atatggtagc aaatggtaca
     9661 tcaaagtcaa ttagtgatat ttcagcaaaa gattcagaaa caggaaaata taaatttact
     9721 attaagtata ctgatgcatc tggaaaagca acagagctta ctgtagagag tactaatgaa
     9781 aaagatttaa aagatgccaa agctgcatta gaaggtaatt caaaggttaa attgatagct
     9841 ggagatgata gatatgtaac tgcagtggct atagcaaaac aaacaaaata tactgacaat
     9901 atagttatag ttaattcaaa taaactagtt gatggattag cagctacacc acttgctcaa
     9961 tctaaaaaag cacctatatt attagcatct gataatgaaa taccaaaagt aactttagat
    10021 tatataaaag atataattaa gaaaagccca tcagctaaaa tatatatagt aggaggagaa
    10081 tcagcagtat caaatacagc taaaaagcaa ttagaatcag taactaagaa tgttgaaaga
    10141 ctagctggag atgatagaca tacgacttct gtagcagtgg caaaagctat ggggtctttt
    10201 aaagatgcat ttgtagtagg tgcaaaaggg gaggctgatg ctatgagtat agctgctaaa
    10261 gctgctgaac ttaaggctcc tataatagta aatggttgga atgatctttc agcagatgct
    10321 atcaaattga tggatggaaa agagattggc atagttggtg gttctaacaa tgtatctagt
    10381 caaattgaaa atcaacttgc tgatattgat aaagatagaa aagttcaaag agttgaagga
    10441 gaaacaagac acgataccaa tgctaaggtt atagaaacat attatggcaa attagataaa
    10501 ctatatatag caaaagatgg atatggaaat aatggtatgc tagtagatgc attggcagca
    10561 ggacctctag cagcagggaa aggtccaata cttctagcta aaactgacat aacagactca
    10621 caaaagaatg cacttagtaa aaaattaaat cttggtgcag aagtgactca aataggtaat
    10681 ggagttgaat tgacagtaat acagaagata gctaaaatat taggttggta ataatttata
    10741 ctgtggagaa gattccttaa ttaatacagg aatctttttc tataattatg ctatttattg
    10801 taaattataa cattttataa agtaggagga tgtagtagtg gtagatgcaa ctttattttt
    10861 tacaccacat caagatgatg aaaccttaag tatgggaagt gctataattg aacatgttga
    10921 aaaaagtgat acacatgtca tattatgcac agatgggagt aaatctataa taagaaaagt
    10981 tttggatgat ggagggtcat gttcttatca tattaaagat gttcataaat actctttatc
    11041 agaaagtgaa ttttcaaaag atagagatga agaatttaag gacagctgtg aagcaatggg
    11101 tgtaaaagaa agtaatatac atatagaaaa taatagagcc catgatggtg agctaagtaa
    11161 agaaaaggca agggaaataa tacttaagta cttagaagaa tatccagatg caaaagtaaa
    11221 aactgttact ccgtttaaag ctagtggaat acatgaagac catagagcat taggagaggc
    11281 tgcattagag ttatatagag aaggcaaaat aaaagattta agattttatg tagagccata
    11341 tgactataag gattttaaaa aagttaatcc aaatgtagaa gtatggaagg tattacctag
    11401 tcaagaggag aagttattaa gtgcaatgaa tgcatataaa aaatggaatc ctgaaagtgg
    11461 acattatgct ataggatatc attcagtaaa atctcatttt gatgagttag ctacaaataa
    11521 aatacagtat gtacatgctc cataaaatat tacgaaagaa ttaggaggta agaattaatg
    11581 aaaatatcaa aaaagatagt gtctttgtta actatgacat ttttaactgt tacattatat
    11641 ggaaatacat ctaatgcatc tacaaaagac acattaacag gttctggaag atgggaaaca
    11701 gcaataaaaa taagtcaagc aggatggaaa aagtctgaaa atgctgtgct agtaaatgat
    11761 aattcaatag cggatgcttt atcagctact ccatttgcta aagcaaaaga tgcgccaata
    11821 ttattaactc aaagtaataa attggatagt agaacaaaag cagaattaaa aagacttggt
    11881 gtgaaaaatg tatatttaat aggtggttca attgcattaa gttcagagat tgaaaagcag
    11941 ttaaatgcag aaaatataaa ttttgaaaga atatctggaa atagtagata tgatacttct
    12001 ttaaaattag ctgaaaagct agatagggaa aaatctatat ctaaaatagt agtagtaaat
    12061 ggagaaaagg gtcttgctga tgcagtgagt gttggagcta tagctgccca agaaaatatg
    12121 ccaataatac tttctgattc agataatgga actgaagtag ccgataattt tatagatagt
    12181 aaggatatag aaaaatcgta tgtaataggt ggtacatatt ctatttctaa ttctgtagaa
    12241 agaagtttac caaatgcaac aagaatagca ggtagtagta gaagtgagac aaatgcaaaa
    12301 attatagaag aattttataa agatactgat ataaaaaata tttatgtaac aaaagatggt
    12361 acaaagaata agaatgattt aatagattct ttagcagtgg gtgtattagc agctaaaaat
    12421 agttctccaa tagtattagc aggaaataag tttgatacta cccaaaaaga tgtgttaaat
    12481 actaaaacta tagataaagt tactcaaatt ggtggtttag gtaatgaaaa tgttgtagaa
    12541 gatatactag atatacaaga agagactaag tatactgttg aaactattga tgaacttaat
    12601 gctgctataa aaagagcaga tgcaaatgat attataaagt ttaaaccaga gaaagaaaaa
    12661 actataaata attcttttag tattgaaact aaaaaaactg ttactattga attagatgga
    12721 agatatagac aaacaataac tttggatata cctaatggaa aatttaataa ctatgcagaa
    12781 atagaaggtg gagtaaagct aaaaaacata aaaaatgaat cattagttaa taaaggaagt
    12841 atacaggatt tagatatata tgatgaaaat ggttgtaaaa tagagaatga aagttctgga
    12901 gaaatttggt ttgtcactat agttgaagaa gctaatgatg tatatatagt taatagtggt
    12961 gatataacaa aaatatcaaa taattcttct ggtactataa taagaaattc tggaaatatt
    13021 gatacagtta caggaaaaaa agaacctgca ataagtggaa ataaacctaa agtcaatgat
    13081 acagaaaaag aaactaaagc tgctagaggc ttaaatccta gggtagaagc atgttctgtt
    13141 cctaaaaaag attatgttat gataacaatt cctaattcac ctaaagattc aagatataaa
    13201 atttactata gagtagttta taataaacct tatgcaatgg atgttggtga taaaattaat
    13261 attggcgaat ggactgttgc accaacagat gaagagccat ttcttgaaaa agctaaaaat
    13321 ggttgttatg tagaagctgt tgaggtaaat acttcaacga aagaggtttc tagatgggga
    13381 agaactaacg ttacagatga tggattttaa atattaaaat cttttaaaaa caaaaggtag
    13441 acaatttttt gattgtctac tttttatttt taaaaattat cattttacta aacaaataat
    13501 ttaaaactat aacaactata ttagtaaata cttttacaat aacatcattt acaaaaagca
    13561 aatcaatcat tacatacata attaacattt caacgacacc agaaagtagc ctaaaactaa
    13621 caaaagacaa aaattcttta aataagaact ttatttcaat tcttttactt tcaaacacaa
    13681 aaaacttatt agtaacataa gcaaataaaa cagcaagtat ccatgccaaa gcatttgcaa
    13741 ccataaaatt aaataaaata actctagtaa aaaataaata cgaaactata tttacaagag
    13801 tagtaaatgc tccaaaaaat aaatataata ttgtctcttt atactttttt aaaatcaaat
    13861 ataatcctcc taagctaatt taatataaat atacttaaag atactaacaa acattagtat
    13921 agcacatcta ttaataaatc aatatatctt aaaatattat aaaaaataaa actataatat
    13981 tctataatta atatgtactc ataatccata ttttatcaaa ataaaatatg accttaattt
    14041 agaaatctga tataataaaa attaaataca tataaggggg taaacatgag aaagtacaaa
    14101 tcaaaaaaat tgtcaaagct actagcattg ttgacagttt gctttttaat agtgtcaaca
    14161 atacctgtct cagcagaaaa ccataaaact ctagatggag tagaaactgc agagtattct
    14221 gaaagctatc ttcaatacct agaagatgtc aaaagtggag acacagcaaa atataatgga
    14281 gtaatacctt ttccacatga aatggaaggt acaacacttc gaaataaggg tagaagtagt
    14341 cttccatcag catataaatc aagtgtagca tacaacccaa tgaatcttgg tcttactaca
    14401 ccagcaaaaa atcaaggaag tcttaataca tgctggtctt tttcaggtat gtcaacttta
    14461 gaagcatatc ttaaattaaa aggatatgga acgtatgact tatcagaaga acatttaaga
    14521 tggtgggcaa ctggtggaaa atatggatgg aacttagatg atatgtcagg gagttcaaat
    14581 gtaacagcta tagggtatct gacagcatgg gcaggtccta agttagaaaa ggacatacca
    14641 tataatctta aatctgaggc acaaggtgca actaaacctt caaatatgga tactgcacct
    14701 actcaattta atgtaacaga tgttgttcgt cttaataaag ataaggaaac tgtaaaaaat
    14761 gctataatgc aatatggttc agtgacatca ggttatgcac attattcaac atattttaat
    14821 aaagatgaaa cagcatataa ctgtacaaat aagagagctc cattaaatca cgctgtagcg
    14881 atagtaggat gggatgataa ttattcaaaa gataactttg catctgatgt aaaaccagaa
    14941 tcaaatggag catggttagt aaaaagcagt tggggagagt tcaattctat gaaaggattc
    15001 ttctggattt cttatgaaga taagactcta ctaacagata cagataacta cgcaatgaaa
    15061 tcagtatcaa aaccagatag tgataaaaaa atgtaccaac ttgaatatgc tggtcttagt
    15121 aagataatgt caaataaagt aacagcagca aatgtatttg attttagcag agattctgaa
    15181 aaacttgatt ctgttatgtt tgaaacagac tctgtaggag caaaatatga agtatattat
    15241 gcaccagtag taaatggagt tcctcaaaac aactcaatga caaaacttgc aagtggaaca
    15301 gtatcatatt ctggatacat aaatgtacct actaattctt acagcttacc aaaaggtaaa
    15361 ggagcaatag tagtagttat agacaacaca gcaaatccta atagagaaaa atcaacttta
    15421 gcatatgaaa ctgatataga tggatactat ctatatgagg ctaaagcaaa cttaggtgaa
    15481 agctatatac ttcaaaacaa taagtttgaa gatataaata catatagtga attttctcct
    15541 tgtaactttg ttataaaagc tataacaaaa acatcttctg gacaagctac ttcaggagaa
    15601 tctttaactg gagcagatag atatgaaaca gcagttaaag ttagtcaaaa aggatggact
    15661 tcttcacaaa atgcagtatt agtaaatgga gacgcaatag tagatgcttt aacagctaca
    15721 ccatttacag cagcaatcga ctctccaatt cttttaacag gaaaagataa cttagattca
    15781 aaaactaagg cagagttaca aagattagga actaaaaaag tttatctaat aggtggagaa
    15841 aattctttaa gcaagaatgt acaaactcaa cttagtaata tgggtatatc agtagaaaga
    15901 atttcaggta gtgatagata caagaccagt atatctctag ctcaaaagtt aaatagtata
    15961 aaatctgttt cacaagttgc agtggcaaat ggtgtaaatg gacttgcaga tgcaataagt
    16021 gttggtgcag cagctgctga taacaatatg ccaataatac ttactaatga aaagagtgag
    16081 ttgcaaggtg ctgatgagtt tttaaattca tcaaaaataa ctaagtctta tataattggt
    16141 ggaacagcta ctttatcatc aaatttagaa agtaagcttt caaatccaac aagacttgca
    16201 ggaagtaata gaaatgaaac taatgctaag ataatagata aattctaccc tagctcagat
    16261 ttgaaatatg cttttgttgt taaagatggt tcaaagagtc aaggagactt gatagatgga
    16321 ttagcagtag gagcattagg tgctaaaact gattcgccag tagttctagt tggaaataag
    16381 ttagatgaaa gtcaaaaaaa tgtacttaag tctaagaaaa tagaaactcc tattagagtt
    16441 ggtggaaatg gaaatgaaag tgcttttaat gaactaaata ctcttttagg aaaatagtga
    16501 atggaaagat agtaaggata tacattttat tattaaaaaa tattcaatag aagtttataa
    16561 atataaaata acaaaaaggg gtgtctcaaa atgaactttt tagttcgtga ggcactcttt
    16621 tttgttattt tatattttta tcatttaaag aatgaaaaat aggcttgtct atacttttag
    16681 atagttctct tattattgat gaaaaatgta ataattttaa actgtaaaat agtgtaaagt
    16741 tattttataa tataaatata gtaagatttt aaacaaaata agggggaata ataatgaaag
    16801 caccaaaaac tattttaaca atactgacaa tagcgcttac tttaagtagc atttctataa
    16861 taccatcata tgcacttaca gaggaaaaat taataggtaa tggtagatat gaaactgctg
    16921 taaagataag tcaaaaggca tacagttcta gttctaatgt agtgctagtt aatgacaatt
    16981 cattagctga tgcactttca gctacaccat ttgcaaaggc taaaggagca ccaatacttt
    17041 tgacagaaag tgataagcta gatgatagaa ctgaaaaaga gataaaacgt ctaggagcta
    17101 aagatattta tttaatagga ggtactgctg tacttaataa agatatagag aacaagttaa
    17161 aaggtaatgg acttaatgta gaaagaatta atggtaagga tagatatgaa acttcactga
    17221 ttttggctaa taagcttaag gatataaaag atattaagga agttgctgta gtaaatggtg
    17281 aaaaaggatt gagtgatgct gttagcgttg gggcaccagc agctcaaaat aagatgccta
    17341 taattttgtc tagtcctaag gatggagtag aagcatttga taaatttata agagatgaga
    17401 aagttataaa agcatatgta attggaggta caacttcagt ttcaagagct gtagaaaaga
    17461 gtcttcctaa tgcagaaaga ttgagtggga aagatagaaa tgagactaat gctaaggtca
    17521 tagaaaagtt ttatactgat acaaatttaa gtaatttata tgtaactaag gatggtagta
    17581 aaaatgagaa tcaattaata gactcgttag ctgtcggagt attggctgca aaaaatgagt
    17641 ctcctgttgt tttggttggt aataaattaa atacaaaaca aagagatata cttagtacta
    17701 aaaaattaaa tactataaca caggtaggtg gaaatggaaa tgaagaagct tttgatgaaa
    17761 ttaaaagcct acaagaaaaa actgtgtttg aagcaaaaac tgttgaagaa ttaactgata
    17821 tgataaatat agcaagtcct aatgatatta taaattttaa accaaaggag aatactgtaa
    17881 atgaagcttt tagaatggta acaaataaac caataactgt gaatataaag ggagattgtt
    17941 caaagactat tacagtagat atgcctaatg gtgaagtgaa taactatgct actttggtaa
    18001 atgttatagt tagaaatatc ggagaaggtg gatttaataa ccatgataca atcactatat
    18061 tgtcagttcg tgacaaaaat ggtagagtta tagaaaatac tagaaattca gatatagata
    18121 cgttgatgat attagcttct gctaatgata caaaattgat taatgatgga tatataggaa
    18181 aacttataga taattcatct aatagtgata taactaataa tggtactata gataaaaaag
    18241 ttaatcaggt tgaggattta gaggctaaag tagattcaat tgaaaaagct atagattcta
    18301 taagtcagaa agtcaataaa atacaagata tactagataa gcttggattt ctaaagaaat
    18361 ttcttagcta agaatatgta tatttaagtg aagtgtgtgt tgtaattgtt ctaaagttat
    18421 atagttaaac aagtttatat acaaacagac ttatagataa atatgtagtt caatatatat
    18481 ttgaataagc tttggggagg taacatgggg attggaattg aatgtaacaa aaaaataaat
    18541 aaaagtattg acaaaactag aataaagaaa aataatct
//
LOCUS       DAEPXF010000073        18536 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain HB27
            SAMN13376389-rid12479423.denovo.077, whole genome shotgun sequence.
ACCESSION   DAEPXF010000073 DAEPXF010000000
VERSION     DAEPXF010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13376389
            Sequence Read Archive: SRR10518432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 18536)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18536)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001069624.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001069624.1
            Genome Coverage       :: 315x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/15/2021 01:54:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,165
            CDSs (total)                      :: 4,076
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 89
            rRNAs                             :: 10, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 10 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 19 of 69
            Pseudo Genes (incomplete)         :: 44 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 10 of 69
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18536
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13376389-rid12479423.denovo.077"
                     /strain="HB27"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="China: Hebei"
                     /collection_date="2017"
     gene            61..951
                     /locus_tag="KRL67_002104"
                     /pseudo
     CDS             61..951
                     /locus_tag="KRL67_002104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015984854.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
     gene            946..1473
                     /locus_tag="KRL67_002105"
                     /pseudo
     CDS             946..1473
                     /locus_tag="KRL67_002105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861041.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2313 domain-containing protein"
     gene            1461..1808
                     /locus_tag="KRL67_002106"
     CDS             1461..1808
                     /locus_tag="KRL67_002106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH1522981.1"
                     /translation="MQDLETKIGTVNIKIDTTKTELTSNIETTKTELIGKIGDTTQLT
                     TTDKTNIVSALNEVKTSVDSIETTADKTSIKDTDNLFESDNVEGALKEVMQEVKGNRS
                     SIISSINSNLIPM"
     gene            1824..3317
                     /locus_tag="KRL67_002107"
     CDS             1824..3317
                     /locus_tag="KRL67_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH1522982.1"
                     /translation="MATYDVDRGVKLREGEHTDEEIFLRACSYQTGGRTYYGTFEVGN
                     EIKVFELETRLYTATTTNVRYYSTSGSEVMVRDVVMRQNVTAMFVAKPTINIKDNLGI
                     ISDACEIEYTISDGFPELRYNIVYKLNNDIIGQIVNTVDSKYKISLTDEYLSKLSHNS
                     TNHIVIEFNDFNNRNMLTKTVIFTKGNTKPKLNITSYNSTTTIFTAIDTDNNLSKIEW
                     FIDDVLKETITTDLYLEKIINYELTDNAVHTLKIVATDAENATVEKVLSISKEIMPFQ
                     SDASLSDISTKLAEIGEGFKNGKTSIINTLALKNIEASLNNTLVELSEKIKTSFDSSD
                     ASVQDLMNQLTQANNTISQLDSKYKYASGTANARENSSLIACIYDPNTSHTVEETSPY
                     WLDLNGIGFIPDIFFAECEYEPNSDAFYKYFVFAIKNTFSISNNTGFVVNITFNKEYG
                     DRSFKLRGDLYTLGKRHVSMDNTGVRVPALNTLNNLRAYKWHAAKFK"
     gene            3331..3623
                     /locus_tag="KRL67_002108"
                     /pseudo
     CDS             3331..3623
                     /locus_tag="KRL67_002108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426797.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            3623..3805
                     /locus_tag="KRL67_002109"
     CDS             3623..3805
                     /locus_tag="KRL67_002109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015981517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH1522983.1"
                     /translation="MNINNIVVRIVSERILNRGLNPLKNRPFELDDVTNIEYRKAVED
                     YIIEHSGVVEGTEPTK"
     gene            3940..5487
                     /locus_tag="KRL67_002110"
     CDS             3940..5487
                     /locus_tag="KRL67_002110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH1522984.1"
                     /translation="MATVYEFNYTGSEQSITLKPGKYKLECWGACGGTVDASDWTDCT
                     KGGYSKGEIVFKKRTNLQIYVGQSGYEKVTEGSSLTRSGFNGGGVAGKVTTGKFAYSK
                     YGGGATDIRLYHPSATWGNTESLLSRILVAGGGGGMKNNFASARSIGHGGGYVGVNGV
                     GRDRDFCGGGSQYQGGTSYDTEEYHGSLGKGGYGGIGVGGGSGYALTKDSYKPAGYIP
                     TSEYWLENVVMESGGNTTKSDGYAKITLLKSLPFLTVSSYNSTQITFKADHTNPTLLT
                     KIEVFIDDILKETIITDLTTEKTINYILEYNALHTLKIVVTDNANTTAEKVVSISKNI
                     MPLGDDASLQDIASKVKEMKHGLINGKTSIINVLSLKNIESSLNNSLVELSEKVKESF
                     DSSDTSLQDLINQLTQANNTISQLNSKYKYSSGTFTYYTGAYPNGINIGFRPDVISYT
                     VINAVEEKYYCVYTPLFYLFYNYTTGEKYLDVNHNHYGITFTDNGWIPKGPVNNGVYS
                     YIAVKCC"
     gene            5545..5775
                     /locus_tag="KRL67_002111"
     CDS             5545..5775
                     /locus_tag="KRL67_002111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin XhlA family protein"
                     /protein_id="HBH1522985.1"
                     /translation="MNEELFKENLKRHEVTINKHNDEIDELKVANIESKAELKALCEN
                     LNSLTSMLKWLIGTMITTLVGFFIFAIQRGIF"
     gene            5795..6052
                     /locus_tag="KRL67_002112"
     CDS             5795..6052
                     /locus_tag="KRL67_002112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HBH1522986.1"
                     /translation="MDNLISFIPEQLLLLVVALNVLGFGFKKYKQLDNKYIPIILLVL
                     GIVFSIWMLGFNPSSILQGILCWGVAIGANQTYKQLKEENK"
     gene            6052..6798
                     /locus_tag="KRL67_002113"
     CDS             6052..6798
                     /locus_tag="KRL67_002113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HBH1522987.1"
                     /translation="MKIAILTDLIVKWLKQGGATVYTGKVDKSNNYLAEQCQIANRQN
                     VDVAIQIHFNADHTTLDKMGTETIYKTNNGKVYADRVNTKLATVFKNRGAKSDVRGLF
                     WLSHTKAPAILIEVCFVDSKADTDYYIRHKDIVAKLIAEGILNKSINSNSTESGGNNN
                     MDKFDTAIVYSGETDKAIATIMSFYISNSTIVDIKDYKSYMCRNVFVIEGGATEGIKK
                     YPDKYTNFMGADRKETFKLVLEYLKNKKLL"
     gene            7018..7833
                     /locus_tag="KRL67_002114"
     CDS             7018..7833
                     /locus_tag="KRL67_002114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBH1522988.1"
                     /translation="MKTGKYFETVSGIEIHYIDEGKGKPVIFVPGWTFSCDVFEAQVN
                     RFSSEYRVIAVDPRAHGRSTVTDIGVDHTTHAQDIVKLIKHLGLNDVILVGWSFGAYE
                     TWGVIREIGIEHIKGIFNIDMSPKAISTNLGDWVEDSVEALCETIQVILTPQGFKEVL
                     KEYTNEVMIQKTLSEEEMEFIMSCSTMPYYLASAEYAIGVTGDYRKEAKLADESEGCH
                     TEFMIAEHWQDVAVPYMKKLCPNTKLHILGGHMAFWEYADEFNAILSEFLKKC"
     gene            complement(8041..8358)
                     /locus_tag="KRL67_002115"
     CDS             complement(8041..8358)
                     /locus_tag="KRL67_002115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH1522989.1"
                     /translation="MDFGNIYLVLIGIAVIIVTTIRYLIKGKTNYCKKKYLDKLELKY
                     GNIDREKVVKLEIFYQYLIGLEYIAIGLFTRRLDITILAIILVAIITGVFYYLIRKKY
                     ITL"
     gene            complement(8387..9373)
                     /locus_tag="KRL67_002116"
     CDS             complement(8387..9373)
                     /locus_tag="KRL67_002116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HBH1522990.1"
                     /translation="MKLQEKWIDLLNPKYIENKEKRKKFQIIIFFSITFGLTYLLGLL
                     LYFNKFIDPENFALFMMVLPLSSVAIAKFYTEGMTNDKYKFYSLIILFFLIYLFLLII
                     ELLNLINNQQFQSLNTILVIISSLSIILYSDSIKDLSPIKNIKVGCLLIFYFMLSKII
                     PESLRLIIQGNQPNYEGILNYIFSYMMLFFSIYPFFGEEYGWRGFLQDIFFDRLGKKL
                     GILALSMCWSLWHLPLIFTLYTPETPVLGLITRSIYIFGISIFLGYVYMKTKNIWFCA
                     IIHALNNTSFLISASSITYCSTINYSNIVEISILILIFYVPFLFTKEYKQNT"
     gene            complement(9788..9988)
                     /locus_tag="KRL67_002117"
     CDS             complement(9788..9988)
                     /locus_tag="KRL67_002117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH1522991.1"
                     /translation="MCFKTYILKREVNNKQLEITRSFDLDNLICTATEILKDNEIDLI
                     IIDEVNNVVWRNEKSTPAHENA"
     gene            complement(10007..10420)
                     /locus_tag="KRL67_002118"
     CDS             complement(10007..10420)
                     /locus_tag="KRL67_002118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH1522992.1"
                     /translation="MKVKNKRNFIVGIIVCMLCCASLVIYCILKEKRFLISSFILITI
                     AIFNFCNAFSRKGIVEELHNSTDERDLYLTMKTSHILVKIMNYTLFTFTFLFIIAYSA
                     WKNQSLIVIAITLCVIEIFLFVAYLLINIFLEKKE"
     gene            complement(10404..10637)
                     /locus_tag="KRL67_002119"
     CDS             complement(10404..10637)
                     /locus_tag="KRL67_002119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBH1522993.1"
                     /translation="MKTKVREFRTNMGLTQQQLADLVHVSNRTIISIEKEQYSPSLML
                     AYRMAQVFGTTIEELCCLKENKEMEDKKHESKE"
     gene            10856..11230
                     /locus_tag="KRL67_002120"
     CDS             10856..11230
                     /locus_tag="KRL67_002120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HBH1522994.1"
                     /translation="MKISKLPEAELKVMRYIWEANKVLTSRKLVAAMEEKYEWNESTT
                     FTVLKKLERREFLSTQKIGKLTHYNILVKEKNYLRFETKEFLKNIHKNSISSLMSALH
                     GEDDEVDEDKLSELEEYFKNLK"
     gene            11720..12412
                     /locus_tag="KRL67_002121"
     CDS             11720..12412
                     /locus_tag="KRL67_002121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBH1522995.1"
                     /translation="MYNILVVDDDKEIVDSIEIYLKSEGFKIYKAYDGMKALEIITEN
                     NIHLILMDIMMPKLDGIKATVKIREERNIPIILISAKSEDTDKIIGLNIGADDYITKP
                     FNLLELIARVKSNLRRYVTLGNYEDTCKEILKSGALELNTLTKEVKIDGENIKMTPIE
                     YKIIELLLENKGRVFSIDEIYEKVWNEESFNSENTVAVHIRRIREKIEINPKEPRFLK
                     VVWGVGYKIEKF"
     gene            12608..14677
                     /locus_tag="KRL67_002122"
     CDS             12608..14677
                     /locus_tag="KRL67_002122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase"
                     /protein_id="HBH1522996.1"
                     /translation="MDKYRVLKLVSLIIISISFPLTILLVTIGLAEPKYNIQKTSTKS
                     GIFDISDDFYDSNIFDKGTMASLLPTFKLSVGGKLTSKEKGKFQQEGKSTDLSSSDSE
                     YDKIMINEQASEEYLGYLKNIKVYVINTYTGVFYTNTSYSNLYDFKQKTKSYCNVEII
                     NKIGKSSYIKTINGEKFELNNLSLELDEVYEVDEGFEAYISFPEEPTIEDGIVYTNFQ
                     IFKQATEKVRLFFVASLSLAIICLLSIILYSKIKGEVLKRNNVFLLIYSKIPMEFNVI
                     LVSISIAILIMYALAVSYHIDVIALVSLSLLIVSNNIFFINVQLSYLENKMCIFQTSI
                     IVRLILWLIKTVREISNAIKKVSLARKVIVIVIISLALIMVISTLSIGIGLLFSICIF
                     LGFTIYITKRLSYLSYVIEGTERIKKGELDYKIKITGNDNFTSLAENINNIGEGLDRA
                     IEEQVKSERMKSDLITNVSHDLKTPLTSIINYVDLIKKEESIQPEYINDYINVLESKS
                     KRLKLLIEDLFEASRVSSGNIELQISKIDLVQLLRQSIGELEEKLSKNNLYLKLNVPN
                     DKVYIWADGRRMYRVFENLLSNIAKYSLEETRVYIDVYDYGENVKVTMKNISSYELNF
                     DPSEIMERFKRADESRNTEGSGLGLAIARDLVDLQGGKFYIEIDGDLFKANLEFQKYE
                     EEITNIE"
     gene            complement(14883..15206)
                     /locus_tag="KRL67_002123"
     CDS             complement(14883..15206)
                     /locus_tag="KRL67_002123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HBH1522997.1"
                     /translation="MNNNYIFFIIIGMYIVTYLPRVLPMLIFSKKEMPDFLKKIMKFI
                     PVAILTSLTAKDVFFNGDSIYLSIANPKIISFMIVLLVAYKFKSIGISIITGVISIYL
                     FGIIL"
     gene            complement(15199..15900)
                     /locus_tag="KRL67_002124"
     CDS             complement(15199..15900)
                     /locus_tag="KRL67_002124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HBH1522998.1"
                     /translation="MERTQQLRESFIASIPIFLGYIPIGIVGGILLQKSGLSPFQIAL
                     MVTLVFGGSSQFIAASMISTGASVTSIVLTTFIVNLRHFLMSSNLNMYIKNKSPKFIL
                     PFCHTITDETFAVNYEKFTAGNWSDKNAIYLNFFCLISSVLANFIGAFLGESISIDSS
                     ISGFILTSMFIALIVSQIKNKIYIVVFISSIIISVILYALFKSNLVIIVASILASLIG
                     FFIEESYCKKEESYE"
     gene            complement(16178..17563)
                     /locus_tag="KRL67_002125"
     CDS             complement(16178..17563)
                     /locus_tag="KRL67_002125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HBH1522999.1"
                     /translation="MPINSFENYPMNWKPKRPSKGQILYKALAEQLEQDINNGFLLPG
                     TKLPPQRELADFLDVNVSTISRAFKICEKKGLISGVTGSGTFVSYDTRSNLFLMSSNN
                     KITFIEMGTMNPDFTLEEINTLFKHIIKEIDFKTIFQYGQRDGAKWQKEAIAKLIYKA
                     GLETTADSLLPASGGQNAIVAILAGLFQHGDRVGVDPLTYPGIKTAAKMLGIQLIPIK
                     QEDNEISEEGLLYTCKNENIKGLYIIPDYQNPTTHIMSQNGRKMVANIASKYNLIVIE
                     DAIHSLLNETHLNPVASYLPNQTIYITSLSKIIAPSLRLAYISTPKQYREALSSALYN
                     INLSQSYFLTEIAYRMIKSGEADKLINARRKSARRRNKIINQYLSGYNLLGNEECIFR
                     WLILPDGIMAEKFEIQALKEGVQVYASERFAVGKEKPISAIRIAVCATESTEELKAGL
                     SVLKRLLEDEK"
     gene            complement(17671..17814)
                     /locus_tag="KRL67_002126"
     CDS             complement(17671..17814)
                     /locus_tag="KRL67_002126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH1523000.1"
                     /translation="MYDCDNQPKTPEEAVLYTRKRNFNIFKKTIPLYDKEGIKIIGRF
                     EIN"
     gene            complement(18010..18432)
                     /locus_tag="KRL67_002127"
     CDS             complement(18010..18432)
                     /locus_tag="KRL67_002127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3284 domain-containing protein"
                     /protein_id="HBH1523001.1"
                     /translation="MNTFKTEVLLKYSAKDIFNTFTKSARLNFPNFNEKKAVGTFVQQ
                     KNNKRFKVEITNFEKNRVYEIKTSNKRESIITRYELVHIDLENTKLIFIESESERNIF
                     GKINSALVRVLFGKRQPEEFNKFIKKLEMELENNSVNL"
BASE COUNT         7206 a         2178 c         2873 g         6279 t
ORIGIN      
        1 aattaaaaag acgattaata atatagaata gaaggtggtg agtatttagt gtttgagtta
       61 atgacttttg aaaatataat taaaagaatg ttagatagtg taccagacac ttttgataaa
      121 agagaagggt ctataatata taatgctctt gctcctgttg ccatagaact cacagaaaca
      181 tacattgcaa tggatgaatt gttagaccaa acattcgtag atactgccag ttattattat
      241 ttagagaaga gatgtaaaga gagaggtatt acacctttac ctgcaaccaa tacaattgca
      301 aaaggagttt ttaatataga tattcctatt gattcaatat tcaacttagg agagtacaat
      361 tatattgcaa ccgaaaggat tagtgaagga atatataaga tgaaatgtga aactgctgga
      421 actatttttg aacttggtca gttgattcca attggatata tagatggtct tgaaactgct
      481 gagttaacag acatactgat aaatggagaa gatgaagaat ctgaggacag tttaagacaa
      541 agatattatg atagtttaaa ttctcaatct tttgggggga atatacaaaa ctataaggat
      601 gaagttaata aattgcttga cgttggggga gttaaagtat atccagtttg gaacggtgga
      661 ggtactgtta agttagtaat aattaattct aacttcaaag ttccatctag tgatttagtt
      721 aatttagttc aagaagaaat tgaccctcta caaaaccaag gagaaggtct tggattagca
      781 ccaataggtc atagagtcac agttgaagga attacaagta caactataaa tatatcagca
      841 gagataacat ataaaaatgg ctacacttgg gaaaatataa agactatagc agaagaagca
      901 ataaacgact atttaaatga gttaaatatg agttgggaag atgcagaaaa caaagaaata
      961 aaaacactac atgaaaaatt aaatgaccta tcaagtgacc agtttttaga ggatttaact
     1021 ccaagtggca taaaaagatg ggaaaagata atgtctataa atcccaagtc aaatgagact
     1081 ttagaggata gaaggtttag gatttttagt aaatatataa gtaaactacc ttactcagag
     1141 agatttttaa ggaactggct agataatgta gttggagaag gcaattatga attaactatt
     1201 aataatgcta cttataacat acaccttgag agtgatgcta gaaatcaaga ttggtttgag
     1261 gaagttcatt cttttgtaag taatattaag ccatgcaata tgacactaga ttacactaga
     1321 gtgcttataa gcaaagacaa ttatatgaat tttggtataa caaccctaat gggtcaagaa
     1381 ataactatat acccttggag tccaccagat atagaaactt atggagaaat tgatgtatta
     1441 actttgcaac actcaaagat atgcaagact tagaaactaa aataggtact gttaatatta
     1501 aaattgatac aactaaaaca gaattaacaa gcaacataga aactactaaa acagagttaa
     1561 ttggaaaaat aggagataca acacaactta ctacaacaga taaaacaaat atcgttagtg
     1621 cattaaatga ggtaaaaact agtgtagata gtatagaaac aacagcagat aaaacaagta
     1681 taaaagatac agataattta tttgaaagtg acaatgtaga aggagcatta aaagaagtga
     1741 tgcaagaagt aaaaggtaat agaagtagta ttatatcaag tataaacagt aatctgatac
     1801 caatgtagaa aggaggaggt taaatggcta cttatgacgt tgatagaggt gttaaattaa
     1861 gagaaggaga acatacagat gaagaaatat ttcttcgtgc ttgctcatat caaactggtg
     1921 gacgtactta ttatggtacc tttgaagttg gcaacgaaat aaaagtattt gaacttgaaa
     1981 ctagacttta tactgctact acaactaatg tgcgttatta ttcaactagt ggttctgagg
     2041 ttatggttag agatgttgtt atgcgtcaaa acgttactgc aatgtttgta gcaaaaccaa
     2101 ctattaatat aaaagataat ttaggtatta taagtgatgc ttgtgagatt gagtatacta
     2161 taagcgatgg ttttcctgaa ttaagatata atatagttta taaattgaat aatgatatta
     2221 taggtcaaat tgttaatact gttgattcaa aatataaaat aagtcttaca gatgaatatt
     2281 tatctaaatt aagtcataat tcaactaatc atattgttat tgagtttaat gattttaata
     2341 atagaaatat gttaactaaa acagttatat ttacaaaagg taatactaaa ccaaaattaa
     2401 atataacttc ttataactca actactacaa tctttacagc aatagataca gataataatt
     2461 tatctaaaat agagtggttc attgatgatg tattaaaaga aacaataaca acagatttat
     2521 atttagagaa aatcatcaac tatgagctta cagacaatgc agtacacaca ttaaaaatag
     2581 ttgctacaga tgcagaaaat gcaacagttg agaaggtttt gagtataagt aaagagatta
     2641 tgccatttca atctgatgct agtttaagtg atatatcaac taagttagca gagattggag
     2701 aagggtttaa gaatggtaaa acaagtatta taaacacttt agcattaaag aacatagaag
     2761 caagtttaaa taatacctta gttgagttat cagagaaaat taaaacaagt tttgatagtt
     2821 cagatgctag tgtgcaggat ttgatgaatc agctaacaca agctaataac actatatcac
     2881 agttagattc taagtataaa tatgccagtg ggactgctaa tgctagagaa aattcttcct
     2941 taattgcatg tatatatgac cctaatactt ctcacactgt tgaagaaaca agtccttatt
     3001 ggcttgattt aaatggaatt ggatttattc ctgatatatt ttttgctgaa tgtgaatatg
     3061 aaccaaattc agatgctttt tataagtatt ttgtttttgc aattaaaaat actttttcca
     3121 tttctaataa tactgggttt gtagttaata ttacttttaa caaggaatat ggtgatagat
     3181 cttttaaatt acgaggagat ttatataccc ttggcaaaag acatgtttcc atggataata
     3241 ctggagttcg agtacctgct ctaaatactc taaataatct tagagcatat aaatggcatg
     3301 cggcaaaatt taaataaatg agatgataaa atgaatagag caaatagaat aattcacgac
     3361 caaactggta aaatactttt acaaactgga gaagcaacag gagatatatt agagcatgat
     3421 acaataacag aattacatta tattgatgtt gaatatggaa atatagacta tagtaaacag
     3481 tatatagaat ctataaatcc aataacaaaa gaacctattt taaaagatat tccaacctat
     3541 taagcgaaga agaaaagaga atacaagagc tagaaaatca attactaatt gcagaaaatg
     3601 aaaaggtagg aggattatta taatgaatat aaataatatt gtggtaagaa tagtatcaga
     3661 aagaatttta aacagaggat taaacccttt aaaaaacaga ccttttgagt tagatgacgt
     3721 gactaacata gagtacagaa aagcagtaga ggattatatt atagagcata gtggagtggt
     3781 agaaggaaca gaacctacaa aataggtaat gtcctttttt atttcttaaa atatataaat
     3841 tcatttacat tttgtctaca cttcatccac actttgtaga cgtttatagt tttaaaatta
     3901 ggtacaataa aaccataaat taagaaatag gtggattaaa tggcaacagt ttatgaattt
     3961 aattatacag gaagtgaaca aagtattaca ttaaaaccag gaaaatataa attagaatgc
     4021 tggggtgctt gtggtggtac tgtcgatgca agcgattgga ctgattgtac aaaaggtggt
     4081 tattcaaaag gtgaaattgt atttaaaaaa agaactaatc tacaaattta tgtcggtcaa
     4141 tctggttatg agaaagttac tgaaggttca agccttacta gaagtggttt taatggtgga
     4201 ggcgttgctg gcaaagttac tactggtaaa ttcgcttatt ctaaatacgg tggtggagca
     4261 actgatataa gactttatca tcctagtgca acttggggta acactgaaag tttgctttca
     4321 cgcatacttg ttgcaggcgg tggcggaggt atgaaaaata attttgcttc tgctcgttct
     4381 attggtcatg gtggtggtta tgtaggtgtt aatggagttg gtcgtgacag agatttttgt
     4441 ggtggtggtt ctcaatacca aggtggaaca agttacgata cagaagaata ccatggttcg
     4501 ttaggaaaag gaggttatgg tggaatagga gtaggtggtg gaagtggtta cgcactaact
     4561 aaagatagtt ataaacctgc tggatatata ccaacttctg aatattggct agaaaatgta
     4621 gttatggaat caggtggaaa tactacaaaa tcagatggct atgctaaaat aacattactt
     4681 aaaagcttac catttttaac tgtatcttct tataattcca cacagattac atttaaagct
     4741 gaccatacaa accctacatt gttaactaag atagaagtat ttatagatga tatattaaaa
     4801 gagactataa taacagattt aacaacagag aaaacaatta actatatatt agaatataat
     4861 gcactacaca cgcttaaaat agttgttaca gacaatgcca atactacagc agaaaaagtt
     4921 gtaagtataa gcaagaatat aatgccatta ggtgacgatg ccagtctaca agatatagca
     4981 agtaaagtta aagaaatgaa acatggctta ataaatggta aaacttctat cataaatgta
     5041 ttatcattaa agaatattga atcaagtttg aacaattctt tagtagaatt gtcagagaag
     5101 gtaaaagaat cctttgatag ttcagatact agtttgcagg atttaataaa ccagttaaca
     5161 caggctaata atactatatc acagttaaac tctaaatata aatattctag tggaactttt
     5221 acttattata ctggtgcgta tcctaatggg ataaatatag gttttaggcc agatgttatt
     5281 tcatacactg ttatcaatgc tgtagaagaa aaatattatt gtgtatacac acctttattc
     5341 tatctttttt ataattatac aactggagaa aaatatcttg atgttaatca taatcattat
     5401 ggaataactt ttactgacaa tggatggata cctaaaggtc cagttaacaa tggtgtgtat
     5461 agttatatag cagttaaatg ttgttaatta tcaaaggctt agataacttc taagccctat
     5521 ttactattaa ataaggaggt tttcatgaat gaagaacttt tcaaagaaaa tttgaaacga
     5581 catgaggtaa caataaataa acataatgat gaaatagacg aattaaaagt agcaaatata
     5641 gagtctaaag cagagttaaa agcattgtgt gagaatctaa attcacttac aagtatgtta
     5701 aagtggctaa ttggaacaat gattacaaca ctagtagggt tctttatatt tgcaatacaa
     5761 agaggaatat tttaattaat taggaggata agagatggat aatttaataa gttttatacc
     5821 agagcaactg cttttattag tagttgctct taatgtatta ggatttggat ttaagaaata
     5881 taaacaatta gataataaat acattccaat tatattgctt gtgctaggaa tagtattttc
     5941 aatatggatg ctaggattta acccttcatc aattttacaa ggtattttat gttggggagt
     6001 tgcaatagga gcaaatcaaa cttataaaca actaaaggag gaaaataaat aatgaaaata
     6061 gcaatactaa ctgatttaat tgttaaatgg ttgaaacaag gtggagctac tgtatatact
     6121 ggaaaggtag ataagtctaa taactattta gcagaacaat gtcaaatagc caataggcaa
     6181 aatgtagatg tggccataca aatacatttt aatgcagacc atacaacctt ggacaaaatg
     6241 ggcacagaga caatatacaa aactaataat ggtaaagtgt atgcagatag ggtaaatact
     6301 aaattagcaa cagtatttaa aaacagaggt gcaaaatcag acgtaagagg gcttttttgg
     6361 cttagtcata caaaagctcc tgcgatatta atagaagtgt gcttcgtaga tagtaaagca
     6421 gatacagatt attatattag acataaagat atagttgcta aattaatagc agaaggtatt
     6481 ttaaataaga gtattaattc aaacagtaca gaaagtggag gaaataataa catggataaa
     6541 tttgatacag ctatagtcta cagtggggaa accgataagg ctatagctac tattatgtct
     6601 ttttatatta gtaatagtac tatagtagat ataaaagatt ataagagtta tatgtgcaga
     6661 aatgtatttg taatagaggg aggagcaaca gaaggaatta aaaagtatcc cgacaaatac
     6721 acaaacttta tgggagcaga tagaaaagaa acttttaaat tagtattaga atacttaaaa
     6781 aataagaaat tactataaat aaaggtttat agatacaaca ggtaataact tgttattcat
     6841 gaaattagtg tagtggccat catatacaga agttttttgt gtttttttaa aaattttctt
     6901 gtgtatcttt ttagtaagaa tttgaattga tttaaaatga caaaatatga taaactgaaa
     6961 ttagcaatat ttgtaagtag gtatattatt tttggataga agaatggagg agttaaaatg
     7021 aagactggta aatattttga aacagtttct ggtattgaaa tccattatat tgatgaagga
     7081 aaaggaaaac cagttatatt tgttccaggt tggacttttt cttgcgatgt ctttgaagcg
     7141 caggtgaaca gattttcttc tgagtacaga gtaatcgcag ttgatccaag agcacatggt
     7201 cgttctactg tgacagatat tggtgttgac catacaacac atgcacagga tattgtaaag
     7261 cttatcaaac acttaggtct taatgatgta atacttgttg gttggtcttt tggtgcatac
     7321 gaaacatggg gcgtaatccg tgaaattgga atagaacaca ttaaaggaat tttcaatatt
     7381 gatatgtcac caaaagctat ttctacaaat ctaggcgatt gggtggaaga tagtgtggaa
     7441 gcattatgtg agacaataca agtaattctt acacctcaag gattcaaaga agttttgaag
     7501 gagtacacaa atgaggttat gatacaaaaa acactttcag aagaagagat ggaattcatc
     7561 atgagttgtt ctacaatgcc atattatttg gcatcagcag aatatgcaat aggtgtaact
     7621 ggagattaca gaaaggaagc aaaactggca gatgagagcg aaggatgcca tacagaattc
     7681 atgattgctg aacattggca ggatgtagct gtaccgtata tgaagaagct ctgtccgaat
     7741 actaaactcc atattttagg agggcatatg gctttttggg aatatgctga tgagtttaat
     7801 gcaatattgt ctgaattttt gaaaaaatgt tagtgaatgt gggaaggtag tattaaaatt
     7861 gtaaaataca gaactggttt atagactatt catacaaaga tagctcatat tatcatggac
     7921 tatctttgta taaattatat aatgtatagt gcttatacgc cgaattatat agtaaagtga
     7981 gtaaaaactc catagcatac attagtaaaa tggtgtcaac taaatatttg attttagttt
     8041 ttacagagtt atatatttct ttctaattaa atagtaaaat acccctgtta taatggctac
     8101 taatattatt gctaatattg taatatctaa tcttcttgtg aataaaccta ttgctatgta
     8161 ttcaagacct attaaatatt gataaaatat ttctagttta acaacttttt ctctatctat
     8221 atttccatat tttaattcta atttatctaa atattttttc ttacaataat tagttttacc
     8281 tttaattaaa tatcttattg tagttactat tataactgct attccaataa gtacaagata
     8341 gatattacca aaatccatag tagatacctc ctttataata taatctttag gtgttttgtt
     8401 tatattcttt tgtaaataga aatggaacat aaaaaattaa aataagtatt gatatttcta
     8461 ctatattaga ataattaata gttgaacaat aggtaatact agaagctgat ataagaaaag
     8521 atgtattatt taaagcgtgg attatagcac aaaaccatat atttttagtc ttcatgtata
     8581 catatcctaa aaatattgaa atcccaaata tgtatattga gcgagttatt aagcctaata
     8641 ctggtgtttc aggtgtatat aatgtaaata ttagaggtaa atgccacaaa ctccaacaca
     8701 tacttagtgc tagaataccg agttttttac ctaatctatc aaaaaatata tcttgcaaaa
     8761 atcccctcca accatattct tcaccaaaaa aagggtatat agagaagaat aacatcatat
     8821 aagagaatat ataattcaaa ataccttcat agttaggttg atttccttgt attataagcc
     8881 taagtgattc cggaataatt ttggaaagca tgaaataaaa aattagtaaa caacccactt
     8941 taatattttt tataggagac aaatctttta tgctgtcaga ataaagaata atagataaac
     9001 tacttataat aaccaatata gtatttaatg attgaaattg ctgattattt attaagttta
     9061 ataattcaat aataagtaaa aataagtaaa ttagaaaaaa taatattatt aatgaataaa
     9121 atttatattt atcattagtc attccttctg tataaaattt tgcgattgca actgaggata
     9181 agggcaatac catcatgaat aatgcaaaat tttctgggtc aataaattta ttgaaatata
     9241 atagaagacc taataaataa gttaaaccaa aagttataga aaaaaatatt ataatctgaa
     9301 atttctttct tttttctttg ttttcaatat attttggatt taataaatca atccattttt
     9361 cttgcaattt caatataaaa acctccttta aaatatcact ttcttttttt gtaatcacca
     9421 acttatattg tattaaatta aatttcttaa tcaaagtata tcagtaatac aagtataaag
     9481 agtatctaaa atattacaaa tattaacaag acacatcata atctgtaata ataaaaagaa
     9541 tgaaaattat catgctaaaa cataagacat gttataatta tattacatat ttcataaata
     9601 tacatttaaa gcactctttt tatatgagtg cttatttttt tgaaattcat taatatatat
     9661 ataaactatc aagaacatta ctcaacacac cttaaaatcg atttaaataa gtctttttaa
     9721 taacataaat tatatgatat aataaaaaaa atagaagtgt attggtagta cacttctata
     9781 gttataacta agcattctca tgagcgggag tgcttttttc atttctccaa acaacattat
     9841 ttacttcatc aataattatc aaatcaatct cattatcttt caaaatctct gttgcagtac
     9901 aaatgagatt atctaagtca aaacttcgag ttatctctag ttgtttgtta tttacttctc
     9961 tttttagtat ataagtttta aaacacattt atatcacctc ttgtatttac tcttttttct
    10021 ctaaaaatat attgataagt aaatatgcaa caaataaaaa aatctcaatg acacatagtg
    10081 ttatagctat aactataagt gattgatttt tccatgcact ataagctatg atgaaaagaa
    10141 atgtaaaagt aaataatgta taattcataa ttttaactaa gatatgactt gttttcattg
    10201 ttaagtacaa gtctctttca tctgtactat tgtgtaattc ttcaacaatg ccttttcttg
    10261 aaaaagcatt acaaaaatta aagattgcta ttgtaattaa aataaatgat gaaattaaga
    10321 atcttttctc ttttaaaatg caataaataa ctaaactagc acaacataac atacaaacta
    10381 taatacccac tataaaattt cgtttattct ttactttcat gttttttatc ctccatttct
    10441 ttattttctt ttaaacaaca tagttcttca atagttgtac caaatacttg tgccatacga
    10501 taggcaagca taagagaagg gctatattgc tctttttcaa tagaaataat tgttctgttt
    10561 gatacatgga ctaaatcagc gagttgttgc tgtgttagac ccatatttgt tctaaattct
    10621 cgtacttttg ttttcattcc ataacctctt tctatatgaa gctaacttca tgtgaagtta
    10681 acttcataat atcctgaaat gagtaaaatg tcaatctcat tagataagta ttataaaacc
    10741 taaaagaata atatggtaaa aattagatgg atatatttat attaattctc ttgtttggaa
    10801 aactttatta agatagaatt taacatatat ataaatcggc aaaggggtgc gaattatgaa
    10861 aattagtaaa ttaccagaag cggaattaaa agttatgaga tatatttggg aagctaacaa
    10921 ggtattaact tctaggaaat tagttgccgc aatggaagaa aaatatgagt ggaatgaatc
    10981 aactacattt acagttttaa aaaaattaga gagaagggaa tttttaagta cgcagaaaat
    11041 aggtaaactt acacattata atatactagt aaaagaaaaa aactatctaa gatttgaaac
    11101 aaaggaattt ttgaagaaca tacataaaaa ttcaatttca agtttaatgt cagcgttaca
    11161 tggtgaagac gacgaagtgg atgaagataa attaagtgaa ctcgaagaat attttaagaa
    11221 tttgaaataa gaaaatgaca aattgataca taataaataa aatgaaggta tgaaattata
    11281 actttttcta ctttcatttt gtttattatt ctgaacatgt taagaaaatg aaataggttt
    11341 gtctaatatt tatataaatt ttagtagaat atgtatagta tatagtagat agtatgtata
    11401 aaaataaaaa ctgttcaata agaattaata tacattaatt cagctgaatc caatcaaaat
    11461 acttaaataa aagtaatctg aaaataacat aattcaaatc aatttattca aataaacctc
    11521 aatttatctt aaatcttaag aaatatttaa gaattaggat aatattttct taagatttat
    11581 attttattat atagttataa gggaaagtta caaaaatgta agccaaagta atataacaat
    11641 ttagtcattg ttgagaaagg aagtgaaaga tattgtataa tatagataag tattattata
    11701 caatagagga agtgaaaata tgtacaatat attagtagta gacgatgata aagaaatcgt
    11761 tgattcaata gaaatatatc ttaaatcaga aggatttaaa atatataaag catatgatgg
    11821 aatgaaagct ttggaaataa taacagagaa taacattcat ctaatactaa tggatataat
    11881 gatgcctaaa ttggatggaa tcaaagcaac tgtaaaaata cgagaagaaa gaaatattcc
    11941 aataatttta atttcagcaa aaagtgaaga tacagataaa ataataggat taaatattgg
    12001 agctgatgat tatataacaa agccatttaa tctattagag cttatagcta gagttaaatc
    12061 aaacttaaga cgatatgtaa ctttaggtaa ttatgaagat acatgtaaag aaattttaaa
    12121 aagtggagct ttggagttaa atacactaac aaaagaagtt aaaattgatg gtgaaaacat
    12181 aaaaatgact ccaattgagt ataaaataat tgaacttctt ttagaaaata aaggacgagt
    12241 tttctcaata gatgagatat acgaaaaagt atggaatgag gaaagcttta attcagaaaa
    12301 tacagtagca gtacatatca gacgaataag agaaaagatt gaaataaatc caaaagaacc
    12361 aagattttta aaggtggtgt ggggagttgg ttataagatt gaaaaatttt agtggaaaaa
    12421 ttataaaaga aaagagttta atatttaatt gcattttgat tttagtcatt tttacattta
    12481 cacaaatgtt aggaatttta atatgtttta attttttctc tgagagttta atttactcta
    12541 tggggattat gcttttctta aactttattt tatataaaat actgacaatg tagatgaggt
    12601 gggcaatttg gataaatata gagtattaaa gttagtttca ttaatcataa tatctatctc
    12661 atttccatta actatattgt tggtgacaat aggacttgct gagcctaaat acaatataca
    12721 aaaaacttct acaaagagtg ggatttttga tatatctgat gatttttatg atagtaatat
    12781 atttgacaaa ggaacaatgg caagtctatt acccacgttt aagctatctg taggtggtaa
    12841 attaactagt aaagaaaaag gtaaattcca acaagaaggt aaaagtacag atttaagtag
    12901 tagtgattct gagtatgata aaatcatgat taatgaacaa gcttctgaag aatatttagg
    12961 ctatctaaaa aatataaaag tgtatgttat taatacatat acaggagtat tttatacaaa
    13021 tacgtcatat tctaatttat atgattttaa acaaaaaact aagagctatt gtaatgtaga
    13081 gataataaat aagataggaa agagctcata tattaaaaca ataaatggag aaaaatttga
    13141 actgaataat ttgagtctgg aactagatga agtatatgaa gtagatgaag gttttgaggc
    13201 atatatatct tttccagaag agccaactat agaagatgga atagtataca ctaattttca
    13261 aatatttaaa caagctacag aaaaagtaag attatttttt gtagctagtt taagtttagc
    13321 aattatatgt ttattatcta taattttata tagcaaaata aaaggtgaag ttttaaaaag
    13381 aaataatgta tttttattaa tatacagtaa gatacctatg gaatttaatg taatattagt
    13441 atctatttca attgcaattt taattatgta tgcccttgct gtatcttatc atattgatgt
    13501 aattgcatta gtttctttat ctttactgat agtatcaaat aatatatttt ttataaatgt
    13561 gcaattgagt tatttagaaa ataaaatgtg tatatttcag acaagtatta tagtaagatt
    13621 gattttatgg ttaataaaaa ctgtaaggga gatcagtaat gctataaaga aagtatcttt
    13681 agcaaggaaa gttattgtta tagtgataat atctttagct ttaatcatgg taattagcac
    13741 tctaagtata ggtataggat tactgttttc tatatgtatt tttttaggat ttacaatata
    13801 tataactaaa agactttcat atcttagtta cgtaatagaa ggtacagaaa gaataaaaaa
    13861 gggagaatta gattataaga taaaaattac tgggaatgat aattttacgt ctttagctga
    13921 aaatataaat aatattggag aaggtcttga tagagctata gaagagcaag taaaaagtga
    13981 aagaatgaag tctgacctta taacaaatgt atctcatgat ttgaaaactc cacttacttc
    14041 tataataaat tatgtagatt taattaaaaa agaagaatca atacaaccag agtatataaa
    14101 tgattatata aatgtacttg agtcaaagtc aaaaagacta aaattattga tagaagattt
    14161 atttgaagcg agtagagtaa gtagtggcaa tatagaatta caaattagca agattgattt
    14221 agtgcaacta ttaagacaaa gtataggtga acttgaagaa aaattatcta aaaataattt
    14281 atatttaaaa ttaaatgttc caaatgataa ggtgtatata tgggcagatg gaagaagaat
    14341 gtatagagta tttgaaaatc ttttatctaa tatagcaaaa tattccttag aagaaactag
    14401 agtctatatt gatgtatatg actatggtga aaatgtaaag gtaactatga aaaatatatc
    14461 ttcttatgaa ttaaattttg acccaagtga gattatggaa agatttaaaa gagctgatga
    14521 atcaaggaat actgaaggaa gtggattagg gcttgctatt gcaagagatt tagtagattt
    14581 acaaggtgga aaattttata tagaaataga tggagattta tttaaagcaa atttagagtt
    14641 tcaaaaatat gaagaagaaa taacaaatat cgaatgagat agtatctaag tatatatatt
    14701 ttttttactg tcgaaagctt ttattataat atattagaat atttacagta gagaatgaag
    14761 agtaaaaaca gagtaacttt aagtcactct gttttttact taaaatcaat aatataacaa
    14821 cttaaattaa aattgtacaa atagttttaa aataacttat acaataaaac attatccaaa
    14881 tatcataaga taattccaaa taaatatata gatatcactc ctgtaataat agaaatacca
    14941 atagatttaa acttatatgc aacaagaagt acaatcataa aagatataat tttagggtta
    15001 gctatagata aatatatact atctccatta aaaaatacat ctttagcagt taaggaagta
    15061 agtattgcta caggtataaa tttcatgatt tttttcaaaa aatcaggcat ctcttttttt
    15121 gaaaaaatta acataggaag cactcttggt agatatgtaa caatgtacat tccaattata
    15181 ataaaaaata tataattatt attcataaga ttcctccttt ttacagtaag attcttctat
    15241 aaagaaacca attaaagatg ctaaaattga tgcaactatt attactaaat tgcttttgaa
    15301 taatgcatat aaaataacag aaataatgat tgatgatata aatactacaa tataaatctt
    15361 attttttatt tgagaaacta ttaaagctat aaacatagaa gtcaatatga agccagaaat
    15421 agaactgtct atagatatac tttctcctaa aaatgctcca ataaaattag ctaaaactga
    15481 tgaaattaag cagaagaaat tgagataaat agcattttta tcagaccaat ttccagcagt
    15541 aaatttttca taattaactg caaaagtttc atctgttata gtatgacaaa atggcaatat
    15601 aaattttgga cttttatttt ttatgtacat gttcaaatta gaactcatta gaaaatgtct
    15661 taagttaact ataaatgtag tcaaaacaat agatgttaca cttgcaccag tagaaatcat
    15721 tgaagctgca atgaattgag aacttcctcc aaatactaaa gttaccatta gtgctatttg
    15781 aaaaggtgaa agaccagatt tttgtaatag tattcctcct actataccta taggtatata
    15841 acctaagaat ataggaattg aagcaataaa gctttctctt aattgttgag ttctttccac
    15901 aataaatcac tccttttgta ttaaatgtat taaagtatta gtaaaacaga aaaatagatt
    15961 aattgtataa ttgcaattat aattaaaaat aaatttggat taatatatag attactatat
    16021 aacaaataga taatcttaaa ctattattaa attttatcta tttgttatta tgatgtaata
    16081 atacatacta atctctataa attaattact atctggtgta attttatttt aacaacaaaa
    16141 aaaaatacac acaattaaaa tattgtgtgc aatacaatta tttttcatct tctaatagtc
    16201 tttttaatac gctcagacca gctttaagtt cttctgtgct ttctgtagca catacagcta
    16261 ttcttattgc agaaattggt ttttcttttc caacagcaaa acgttcagaa gcatagactt
    16321 gtacaccttc tttaagtgct tgtatttcaa atttttctgc cattattcca tcaggcagaa
    16381 tcaaccatct aaatatacat tcttcatttc ccaaaagatt atatccagat aaatattgat
    16441 taataatctt atttcttctt cttgcagatt tacggcgtgc attaattaat ttatcggctt
    16501 cacctgattt aatcatacga taagcaattt ctgttaaaaa ataagattga gataaattaa
    16561 tgttgtataa tgcgcttgat aatgcctctc gatactgttt aggtgtagag atatatgcta
    16621 gccgtaaact aggtgcaata atttttgata aactcgtaat ataaattgtt tgattgggca
    16681 gataggaagc cactggattt aaatgtgttt cattaagtaa actatgaata gcatcctcaa
    16741 taacaattaa attgtattta gatgcaatat ttgcaaccat ttttcgtcca ttttgtgaca
    16801 ttatatgtgt agttggattt tgataatctg gtattatgta tagaccttta atattttcat
    16861 ttttacaagt atataatagt ccttcttcac taatttcatt atcttcttgt ttaattggaa
    16921 ttagttgaat cccaagcatt ttagctgctg tctttattcc aggataagtt aatgggtcta
    16981 cacctactct atctccatgt tgaaacaaac ctgctaaaat agcaacaatt gcattttgcc
    17041 caccacttgc aggaagtaag ctatctgctg tagtttcaag cccagcttta taaattaatt
    17101 tggcaatagc ttctttttgc catttagcac catctctttg tccatattgg aatatagttt
    17161 taaaatctat ttctttaatt atgtgtttaa acaaagtatt aatttcttct agtgtgaaat
    17221 ctggattcat tgtgcccatt tcaataaatg taattttatt attactggac atcaagaata
    17281 gatttgaacg cgtatcataa gatacaaaag ttccacttcc agtaactcca cttattagac
    17341 cttttttttc acaaatttta aaagctctag agatagtact cacattaaca tctaagaaat
    17401 ctgccaattc acgttggggt ggtaatttag tacctggtaa taaaaatcca ttgttgatat
    17461 cttgttctag ttgttctgca agagccttat ataatatttg cccttttgaa ggtctctttg
    17521 gtttccagtt cataggataa ttttcaaaag aattaattgg cataagtttt cacctcacat
    17581 tattaatttg attttttctt tatctaaaac aataaattct aaggatacaa ataaaaactg
    17641 ataggaaata attctatcag tttttgtctt ttaattgatt tcaaatcttc ctataatttt
    17701 tataccttct ttatcataaa gagggattgt ttttttaaat atattaaaat ttctttttct
    17761 tgtatataaa acagcttctt ctggtgtctt tggttgatta tcacaatcgt acaaatctct
    17821 tttttttaaa ctaaaagcaa taatacataa aattattatt gctaatgata ctcctaatgt
    17881 ggtatatatt catagtgtca catgtctatc aatgtttttt tcagtaatta ttgtaacaaa
    17941 tagtagaacc cataaaatac ttaaaaatat gtttttagat agcttttaaa tatataatgt
    18001 aacttatatc tataaattaa cactattatt ctctaattcc atttctagtt tttttataaa
    18061 tttattaaat tcttctggtt gcctttttcc aaacaatact cttacaagag ctgaattgat
    18121 ttttccaaat atatttctct cgctctcact ttcaataaaa ataagttttg tattttctaa
    18181 gtcaatatgt accaattcat atctagtaat aatactttct cttttgttgc ttgtttttat
    18241 ctcatatact ctattttttt cgaaattagt tatttcaacc ttaaatcttt tattattttt
    18301 ttgttgtaca aatgtgccta ctgctttttt ctcattaaaa ttaggaaaat ttagtcttgc
    18361 agattttgta aatgtattaa atatgtcttt tgcagaatat tttaataaaa cttcagtttt
    18421 aaatgtattc atatcgtttt cccttctgta tataaattta atatttataa gttaattttt
    18481 aagttaaaat agtataatat gccatattaa atcatgttaa aactatttat atagct
//
LOCUS       DAEPXF010000074        17782 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain HB27
            SAMN13376389-rid12479423.denovo.078, whole genome shotgun sequence.
ACCESSION   DAEPXF010000074 DAEPXF010000000
VERSION     DAEPXF010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13376389
            Sequence Read Archive: SRR10518432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 17782)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17782)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001069624.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001069624.1
            Genome Coverage       :: 315x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/15/2021 01:54:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,165
            CDSs (total)                      :: 4,076
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 89
            rRNAs                             :: 10, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 10 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 19 of 69
            Pseudo Genes (incomplete)         :: 44 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 10 of 69
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17782
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13376389-rid12479423.denovo.078"
                     /strain="HB27"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="China: Hebei"
                     /collection_date="2017"
     gene            249..7352
                     /gene="tcdB"
                     /locus_tag="KRL67_002128"
                     /gene_synonym="toxB"
     CDS             249..7352
                     /gene="tcdB"
                     /locus_tag="KRL67_002128"
                     /gene_synonym="toxB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosylating toxin TcdB"
                     /protein_id="HBH1523002.1"
                     /translation="MSLVNRKQLEKMANVRFRVQEDEYVAILDALEEYHNMSENTVVE
                     KYLKLKDINSLTDTYIDTYKKSGRNKALKKFKEYLVIEILELKNSNLTPVEKNLHFIW
                     IGGQINDTAINYINQWKDVNSDYNVNVFYDSNAFLINTLKKTIIESASNDTLESFREN
                     LNDPEFNHTAFFRKRMQIIYDKQQNFINYYKAQKEENPDLIIDDIVKTYLSNEYSKDI
                     DELNAYIEESLNKVTENSGNDVRNFEEFKTGEVFNLYEQELVERWNLAGASDILRVAI
                     LKNIGGVYLDVDMLPGIHPDLFKDINKPDSVKTAVDWEEMQLEAIMKHKEYIPEYTSK
                     HFDTLDEEVQSSFESVLASKSDKSEIFLPLGDIEVSPLEVKIAFAKGSIINQALISAK
                     DSYCSDLLIKQIQNRYKILNDTLGPIISQGNDFNTTMNNFGESLGAIANEENISFIAK
                     IGSYLRVGFYPEANTTITLSGPTIYAGAYKDLLTFKEMSIDTSILSSELRNFEFPKVN
                     ISQATEQEKNSLWQFNEERAKIQFEEYKKNYFEGALGEDDNLDFSQNTVTDKEYLLEK
                     ISSSTKSSERGYVHYIVQLQGDKISYEAACNLFAKNPYDSILFQKNIEDSEVAYYYNP
                     TDSEIQEIDKYRIPDRISDRPKIKLTFIGHGKAEFNTDIFAGLDVDSLSSEIETAIGL
                     AKEDISPKSIEINLLGCNMFSYSVNVEETYPGKLLLRVKDKVSELMPSMSQDSIIVSA
                     NQYEVRINSEGRRELLDHSGEWINKEESIIKDISSKEYISFNPKENKIIVKSKNLPEL
                     STLLQEIRNNSNSSDIELEEKVMLAECEINVISNIETQVVEERIEEAKSLTSDSINYI
                     KNEFKLIESISDALCDLKQQNELEDSHFISFEDISETDEGFSIRFINKETGESIFVET
                     EKTIFSEYANHITEEISKIKGTIFDTVNGKLVKKVNLDTTHEVNTLNAAFFIQSLIEY
                     NSSKESLSNLSVAMKVQVYAQLFSTGLNTITDAAKVVELVSTALDETIDLLPTLSEGL
                     PIIATIIDGVSLGAAIKELSETSDPLLRQEIEAKIGIMAVNLTTATTAIITSSLGIAS
                     GFSILLVPLAGISAGIPSLVNNELVLRDKATKVVDYFKHVSLVETEGVFTLLDDKVMM
                     PQDDLVISEIDFNNNSIVLGKCEIWRMEGGSGHTVTDDIDHFFSAPSITYREPHLSIY
                     DVLEVQKEELDLSKDLMVLPNAPNRVFAWETGWTPGLRSLENDGTKLLDRIRDNYEGE
                     FYWRYFAFIADALITTLKPRYEDTNIRINLDSNTRSFIVPIITTEYIREKLSYSFYGS
                     GGTYALSLSQYNMGINIELSESDVWIIDVDNVVRDVTIESDKIKKGDLIEGILSTLSI
                     EENKIILNSHEINFSGEVNGSNGFVSLTFSILEGINAIIEVDLLSKSYKLLISGELKI
                     LMLNSNHIQQKIDYIGFNSELQKNIPYSFVDSEGKENGFINGSTKEGLFVSELPDVVL
                     ISKVYMDDSKPSFGYYSNNLKDVKVITKDNVNILTGYYLKDDIKISLSLTLQDEKTIK
                     LNSVHLDESGVAEILKFMNRKGSTNTSDSLMSFLESMNIKSIFVNFLQSNIKFILDAN
                     FIISGTTSIGQFEFICDENNNIQPYFIKFNTLETNYTLYVGNRQNMIVEPNYDLDDSG
                     DISSTVINFSQKYLYGIDSCVNKVVISPNIYTDEINITPVYETNNTYPEVIVLDANYI
                     NEKINVNINDLSIRYVWSNDGNDFILMSTSEENKVSQVKIRFVNVFKDKTLANKLSFN
                     FSDKQDVPVSEIILSFTPSYYEDGLIGYDLGLVSLYNEKFYINNFGMMVSGLIYINDS
                     LYYFKPPVNNLITGFVTVGDDKYYFNPINGGAASIGETIIDDKNYYFNQSGVLQTGVF
                     STEDGFKYFAPANTLDENLEGEAIDFTGKLIIDENIYYFEDNYRGAVEWKELDGEMHY
                     FSPETGKAFKGLNQIGDDKYYFNSDGVMQKGFVSINDNKHYFDDSGVMKVGYTEIDGK
                     HFYFAENGEMQIGVFNTEDGFKYFAHHNEDLGNEEGEEISYSGILNFNNKIYYFDDSF
                     TAVVGWKDLEDGSKYYFDEDTAEAYIGLSLINDGQYYFNDDGIMQVGFVTINDKVFYF
                     SDSGIIESGVQNIDDNYFYIDDNGIVQIGVFDTSDGYKYFAPANTVNDNIYGQAVEYS
                     GLVRVGEDVYYFGETYTIETGWIYDMENESDKYYFDPETKKACKGINLIDDIKYYFDE
                     KGIMRTGLISFENNNYYFNENGEMQFGYINIEDKMFYFGEDGVMQIGVFNTPDGFKYF
                     AHQNTLDENFEGESINYTGWLDLDEKRYYFTDEYIAATGSVIIDGEEYYFDPDTAQLV
                     ISE"
     gene            7474..7974
                     /gene="tcdE"
                     /locus_tag="KRL67_002129"
     CDS             7474..7974
                     /gene="tcdE"
                     /locus_tag="KRL67_002129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holin-like glycosylating toxin export protein
                     TcdE"
                     /protein_id="HBH1523003.1"
                     /translation="MHSSSPFYISNGNKIFFYINLGGVMNMTISFLSEHIFIKLVILT
                     ISFDTLLGCLSAIKSRKFNSSFGIDGGIRKVAMIACIFFLSVVDILTKFNFLFMLPQD
                     CINFLRLKHLGISEFFSILFILYESVSILKNMCLCGLPVPKRLKEKIAVLLDAMTDEM
                     NAKDEK"
     gene            8303..8476
                     /locus_tag="KRL67_002130"
     CDS             8303..8476
                     /locus_tag="KRL67_002130"
                     /inference="COORDINATES: protein motif:HMM:NF022413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH1523004.1"
                     /translation="MRDEGQNDRENKYQTLINDLIDSLKVIGKIYKEIKTLKKYKICS
                     IIQSPYLIEESIN"
     gene            8693..8833
                     /locus_tag="KRL67_002131"
     CDS             8693..8833
                     /locus_tag="KRL67_002131"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH1523005.1"
                     /translation="MSLISKEELIKLAYSIRPRENEYKTILTNLDEYNKLTTNNNENK
                     YL"
     gene            8867..9124
                     /locus_tag="KRL67_002132"
     CDS             8867..9124
                     /locus_tag="KRL67_002132"
                     /inference="COORDINATES: protein motif:HMM:NF024320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH1523006.1"
                     /translation="MNKYKNSSRNRALSNLKKDILKEVILIKNSNTSPVEKNLHFVWI
                     GGEVSDIALEYIKQWADINAEYNIKLWYDSEAFLVNTLKRL"
     gene            9094..9519
                     /locus_tag="KRL67_002133"
     CDS             9094..9519
                     /locus_tag="KRL67_002133"
                     /inference="COORDINATES: protein motif:HMM:NF024321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH1523007.1"
                     /translation="MLSQYTKKAIVESSTTEALQLLEEEIQNPQFDNMKFYKKRMEFI
                     YDRQKRFINYYKSQINKPTVPTIDDIIKSHLVSEYNRDETLLESYRTNSLRKINSNHG
                     IDIRANSLFTEQELLNIYSQDLLNRGNLAAASDIVRLLA"
     gene            9552..10559
                     /locus_tag="KRL67_002134"
     CDS             9552..10559
                     /locus_tag="KRL67_002134"
                     /inference="COORDINATES: protein motif:HMM:NF024321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH1523008.1"
                     /translation="MLPGIHSDLFKTISRPSSIGLDRWEMIKLEAIMKYKKYINNYTS
                     ENFDKLDQQLKDNFKLIIESKSEKSEIFSKLENLNVSDIEIKIAFALGSVINQALISK
                     QGSYLTNLVIEQIKNRYQFLNQHLNPAIESDNNFTDTTKIFHDSLFNSATAENSMFLT
                     KIAPYLQVGFMPEARSTISLSGPGAYASAYYDFINLQENTIEKTLKASDLIEFKFPEN
                     NLSQLTEQEINSLWSFDQASAKYQFEKCVRDYTGGSLSEDNGVDFNKNTALDKNYLLN
                     NKMPSNNVEEAGSKNYVHYIIQLQGDDISYEATCNLFSKNPKNSIIIQRNMNESAKSY
                     F"
     gene            10577..13081
                     /locus_tag="KRL67_002135"
     CDS             10577..13081
                     /locus_tag="KRL67_002135"
                     /inference="COORDINATES: protein motif:HMM:NF023141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH1523009.1"
                     /translation="MLELNKYRIPERLKNKEKVKVTFIGHGKDEFNTSEFARLSVDSL
                     SNEISSFLDTIKLDISPKNVEVNLLGCNMFSYDFNVEETYPGKLLLSIMDKITSTLPY
                     VNKNSITIGANQYEVRINSEGRKELLAHSGKWINKEEAIMSDLSSKEYIFFDSIDNKL
                     KAKSKNIPGLASISEDIKTLLLDASVSPDTKFILNNLKLNIESSIGDYIYYEKLEPVK
                     NIIHNSIDDLIDEFNLLENVSNELYELKKLNNLDEKYLISFEDISKNNSTYSVRFINK
                     SNGESIYVETEKEIFSKYSEHITKEISTIKNSIITDVNSNLLDNIQLDHTSQVNTLNA
                     AFFIQSLIDYSSNKDVLNDLSTSVKVQLYAQLFSTGLNTIYDSIQLVNLISNAVNDTI
                     NVLPTITEGIPIVSTILDGINLGAAIKELLDEHDPLLKKELEAKVGVLAINMSLSIAA
                     TVASIVGIGAEVTIFLLPIAGISAGILSLVNNELILHDKATSVVNYFNHLSESKKYGP
                     LKTEDDKILVPIDDLVISEIDFNNNSIKLGTCNILAMEGGSGHTVTGNIDHFFSSPSI
                     SSHIPSLSIYSAIGIETENLDFSKKIMMLPNAPSRVFWWETGAVPGLRSLGNDGTRLL
                     DSIRDLYPGKFYWRFYAFFDYAITTLKPVYEDTNIKIKLDKDTRNFIMPTITTNEIRN
                     KLSYSFDGAGGTYSLLLSSYPISTNINLSKYDLWIFNIDNEVREISIENGTIKKGKLI
                     KDVLSKIDINKNKLIIGNQTIDFSGDIDNKDRYIFLTCELDDKISLIIEINLVAKSYS
                     LLLSGDKNYLISNLSNTIEKINTLGLDSKKYSVQLH"
     gene            13065..15002
                     /locus_tag="KRL67_002136"
     CDS             13065..15002
                     /locus_tag="KRL67_002136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein"
                     /protein_id="HBH1523010.1"
                     /translation="MYNYTDESNNKYFGAISKTSQKSIIHYKKDSKNILEFYNGSTLE
                     FNSKDFIAEDINVFMKDDINTITGKYYVDNNTDKSIDFSISLVNKNQVKVNGLYLNES
                     VYSSYLDFVKNSDGHHNTSNFMNLFLDNISFWKLFGFENINFVIDKYFTLVGKTNLGY
                     VEFICDNNKNIDIYFGEWKTSSSKSTIFSGNGRNVVVEPIYNPDTGEDISTSLDFSYE
                     PLYGIDRYINKVLIAPDLYTSLININTNYYSNEYYPEIIVLNPNTFHKKVNINLDSSS
                     FEYKWSTEGSDFILVRYLEESNKKILQKIRIKGILSNTKSFNKMSIDFKDIKKLSLGY
                     IMSNFKSFNSENELDRDHLGFKIIDNKTYYYDEASKLVKGLININNSLFYFDPIESNL
                     VTGWQTINGKKYYFDINTGAASTSYKIINGKHFYFNNNGVMQLGVFKGPDGFEYFAPA
                     NTQNNNIEGQAIVYQSKFLTLNGKKYYFDNDSKAVTGWQTIDGKKYYFNLNTAEAATG
                     WQTIDGKKYYFNTNTSIASTGYTIINGKHFYFNTDGIMQIGVFKGPNGFEYFAPANTD
                     ANNIEGQAIRYQNRFLYLHDNIYYFGNNSKAVTGWQTINGNVYYFMPDTAMAAAGGLF
                     EIDGVIYFFGVDGVKAPGIYG"
     gene            complement(15337..16035)
                     /gene="tcdC"
                     /locus_tag="KRL67_002137"
     CDS             complement(15337..16035)
                     /gene="tcdC"
                     /locus_tag="KRL67_002137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosylating toxin anti-sigma factor TcdC"
                     /protein_id="HBH1523011.1"
                     /translation="MFSKKNEGNEFSNEGKGSSKKIIKFFKSTKGIALLVFILGVFFG
                     NISSPACSEDHEKVISNQTSVIDSQKTEIETLNSKLSDAEPWFKMKDDEKKAIEAENQ
                     RKAEEAKKAEEAKKAEEQRKKEEEEKKGYDTGITYDQLARTPDDYKYKKVKFEGKVIQ
                     VIEDGDEVQIRLAVSGNYDKVVLCSYKKSITPSRVLEDDYITIRGISAGTITYESTMG
                     GNITIPGIAVEKIN"
     gene            16452..16697
                     /locus_tag="KRL67_002138"
     CDS             16452..16697
                     /locus_tag="KRL67_002138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Pathogenicity locus"
                     /protein_id="HBH1523012.1"
                     /translation="MGELQKIPGVGKATEKSLIMLGYTTIKSLKDANPAQMYEKECLM
                     RGQHIDRCQLYVYRCAVYFASTENPEPEKLKWWYWKD"
     gene            complement(16915..17184)
                     /locus_tag="KRL67_002139"
     CDS             complement(16915..17184)
                     /locus_tag="KRL67_002139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3795 domain-containing protein"
                     /protein_id="HBH1523013.1"
                     /translation="MFESRCGVCCDSCTKKEQVNCTGCPTMEKPFWGGECKVKTCCES
                     KELNHCGECDTFPCDMLLNKGKDQGFDPMVNIEQCRKWLEETVSN"
     gene            complement(17316..17618)
                     /locus_tag="KRL67_002140"
     CDS             complement(17316..17618)
                     /locus_tag="KRL67_002140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH1523014.1"
                     /translation="MEYSKGFKVIYSLVEYSKRFKVIYCVMEYSKRFKVIYCVMEYLK
                     GFKVIYGVMEYSKRFKAIYDVMEYSKRFKSIYSFTKYSNGFEVIYCALKYSKIFLE"
BASE COUNT         7096 a         2014 c         2666 g         6006 t
ORIGIN      
        1 atatcttaat ttatatattt tatatacaag aatgtttaca tatttatttc aggcaacatc
       61 tttattcaat cgaagagcaa attaatcagc tgagtatctt caatttaaga tgttaggaag
      121 tgaatgtata tgaaaaccta agtagatatt agtatatttt atacatagaa aggaggatat
      181 ataaaagagt tttagcattt agatgtaaaa atatttaata aaaatattat agtaaaggag
      241 aaaattttat gagtttagtt aatagaaaac agttagaaaa aatggcaaat gtaagatttc
      301 gtgttcagga agatgaatat gtagcaatat tagatgcatt agaagaatat cataatatgt
      361 cagaaaatac tgtagttgaa aagtatctaa aattaaaaga tataaacagt ttaacagata
      421 cttatataga tacatataaa aaatctggtc gaaataaagc cttaaaaaaa tttaaagagt
      481 acttagttat agagatatta gaattaaaaa atagcaattt aactccagtc gagaaaaatt
      541 tacattttat atggattgga gggcaaataa atgatactgc tattaattat ataaatcaat
      601 ggaaagatgt aaatagtgac tataatgtta atgtttttta tgatagtaat gcatttttga
      661 taaacacatt gaaaaaaact ataatagaat cagcatcaaa tgataccctt gaatcattta
      721 gagaaaattt aaatgatcct gaatttaacc acactgcatt tttcagaaaa cgtatgcaaa
      781 taatctatga taaacagcaa aatttcataa attactataa agctcaaaaa gaagaaaatc
      841 ctgaccttat aattgatgat attgtaaaga catatctttc aaacgagtat tcaaaggata
      901 tagatgaact taatgcttat attgaagagt cattaaacaa agtcacagaa aatagtggaa
      961 atgatgttag aaactttgaa gaatttaaaa ctggagaagt attcaattta tatgaacaag
     1021 agttagtaga aagatggaat cttgctggtg catctgatat attaagagtc gctatattga
     1081 aaaatattgg tggagtctat ctagatgttg atatgttacc aggaatacac ccagatttat
     1141 ttaaagatat aaataagcct gattcagtaa agacagctgt agattgggaa gagatgcagt
     1201 tagaagccat aatgaaacat aaagaatata taccagaata tacttcaaaa cattttgata
     1261 cattggatga agaagttcaa agtagctttg aatctgtttt agcttctaag tctgataagt
     1321 cagaaatatt tttaccacta ggagatatag aggtatcacc tttagaagta aaaattgcat
     1381 ttgccaaagg ttctattata aatcaagctc taatttctgc aaaagattca tattgtagtg
     1441 acttactaat aaaacaaatc caaaacagat ataagatact gaatgatact ttaggtccaa
     1501 ttattagtca aggtaatgat tttaatacta caatgaacaa ttttggtgaa agtttgggag
     1561 ctatagctaa tgaagagaat ataagtttta tagcaaaaat cggaagttat ttaagggttg
     1621 gattttatcc tgaagctaat actacaatta ctttaagtgg tcctacaata tatgcaggag
     1681 cttataaaga tttattaaca tttaaagaga tgagcataga tacttctata ttgtcatctg
     1741 agttaagaaa ttttgaattt cctaaggtta atatatctca agcaacagaa caagagaaaa
     1801 atagtttatg gcaatttaat gaagaaagag ctaaaattca atttgaagaa tacaagaaaa
     1861 attattttga aggtgcactt ggagaagatg ataatcttga tttttctcaa aatacagtaa
     1921 ctgacaaaga atatctttta gaaaagatct cttcatcaac gaagagttca gaaagaggat
     1981 atgttcatta tattgttcaa ttacaaggag ataaaattag ctatgaagca gcatgtaact
     2041 tatttgcaaa aaatccttat gacagtatac tatttcaaaa aaatatagaa gattcagaag
     2101 tagcatatta ctataatcct acagatagtg aaatacaaga aattgataag tatagaattc
     2161 ctgatagaat ctctgataga cctaagatta aattaacatt cattggtcat ggcaaagctg
     2221 aatttaatac tgatatattt gcaggtcttg atgtagattc attatcttca gaaatagaaa
     2281 cagcaatagg tttagccaaa gaggatattt ctcctaaatc tatagaaata aacttactgg
     2341 gatgtaacat gtttagctat tctgtaaatg tagaagagac ttatcctggg aaattattac
     2401 ttagagttaa agataaagta tcagaattaa tgccatctat gagtcaagac tctattatag
     2461 taagtgcaaa tcaatatgaa gttagaataa atagtgaagg aagaagagaa ttattagacc
     2521 attctggtga atggataaac aaagaagaaa gtattataaa ggatatttca tcaaaagaat
     2581 atatatcatt taatcctaaa gagaataaaa ttatagtaaa atctaaaaat ttacctgaat
     2641 tatctacatt attacaagaa attagaaata attctaattc aagtgatatt gaactagaag
     2701 aaaaagtaat gttagcagaa tgtgagataa atgttatttc aaatatagag acacaagtgg
     2761 tagaagaaag aattgaagaa gctaaaagct taacttctga ctctattaat tatataaaga
     2821 atgaatttaa actaatagaa tctatttctg atgcactatg tgacttaaaa caacagaatg
     2881 aattagaaga ttctcatttt atatcttttg aggacatatc agagactgat gaggggttta
     2941 gtataagatt tattaataaa gaaactggag aatctatatt tgtagaaact gaaaaaacaa
     3001 tattctctga atatgctaat catataactg aagagatttc taagataaaa ggtactatat
     3061 ttgatactgt aaatggtaag ttagtaaaaa aagtaaattt agatactaca cacgaagtaa
     3121 atactttaaa tgctgcattt tttatacaat cattaataga atataatagt tctaaagaat
     3181 ctcttagtaa tttaagtgta gcaatgaaag ttcaagttta cgctcaatta tttagtactg
     3241 gtttaaatac tattacagat gcagccaaag ttgttgaatt agtatcaact gcattagatg
     3301 aaactataga cttacttcct acattatctg aaggattacc tataattgca actattatag
     3361 atggtgtaag tttaggtgca gcaatcaaag agctaagtga aacgagtgac ccattattaa
     3421 gacaagaaat agaagctaag ataggtataa tggcagtaaa tttaacaaca gctacaactg
     3481 caatcattac ttcatctttg gggatagcta gtggatttag tatactttta gttcctttag
     3541 caggaatttc agcaggtata ccaagcttag taaacaatga acttgtactt cgagataagg
     3601 caacaaaggt tgtagattat tttaaacatg tttcattagt tgaaactgaa ggagtattta
     3661 ctttattaga tgataaagta atgatgccac aagatgattt agtgatatca gaaatagatt
     3721 ttaataataa ttcaatagtt ttaggtaaat gtgaaatctg gagaatggaa ggtggttcag
     3781 gtcatactgt aactgatgat atagatcact tcttttcagc accatcaata acatatagag
     3841 agccacactt atctatatat gacgtattgg aagtacaaaa agaagaactt gatttgtcaa
     3901 aagatttaat ggtattacct aatgctccaa atagagtatt tgcttgggaa acaggatgga
     3961 caccaggttt aagaagctta gaaaatgatg gcacaaaact gttagaccgt ataagagata
     4021 actatgaagg tgagttttat tggagatatt ttgcttttat agctgatgct ttaataacaa
     4081 cattaaaacc aagatatgaa gatactaata taagaataaa tttagatagt aatactagaa
     4141 gttttatagt tccaataata actacagaat atataagaga aaaattatca tattctttct
     4201 atggttcagg aggaacttat gcattgtctc tttctcaata taatatgggt ataaatatag
     4261 aattaagtga aagtgatgtt tggattatag atgttgataa tgttgtgaga gatgtaacta
     4321 tagaatctga taaaattaaa aaaggtgatt taatagaagg tattttatct acactaagta
     4381 ttgaagagaa taaaattatc ttaaatagcc atgagattaa tttttctggt gaggtaaatg
     4441 gaagtaatgg atttgtttct ttaacatttt caattttaga aggaataaat gcaattatag
     4501 aagttgattt attatctaaa tcatataaat tacttatttc tggcgaatta aaaatattga
     4561 tgttaaattc aaatcatatt caacagaaaa tagattatat aggattcaat agcgaattac
     4621 agaaaaatat accatatagc tttgtagata gtgaaggaaa agagaatggt tttattaatg
     4681 gttcaacaaa agaaggttta tttgtatctg aattacctga tgtagttctt ataagtaagg
     4741 tttatatgga tgatagtaag ccttcatttg gatattatag taataatttg aaagatgtca
     4801 aagttataac taaagataat gttaatatat taacaggtta ttatcttaag gatgatataa
     4861 aaatctctct ttctttgact ctacaagatg aaaaaactat aaagttaaat agtgtgcatt
     4921 tagatgaaag tggagtagct gagattttga agttcatgaa tagaaaaggt agtacaaata
     4981 cttcagattc tttaatgagc tttttagaaa gtatgaatat aaaaagtatt ttcgttaatt
     5041 tcttacaatc taatattaag tttatattag atgctaattt tataataagt ggtactactt
     5101 ctattggcca atttgagttt atttgtgatg aaaataataa tatacaacca tatttcatta
     5161 agtttaatac actagaaact aattatactt tatatgtagg aaatagacaa aatatgatag
     5221 tggaaccaaa ttatgattta gatgattctg gagatatatc ttcaactgtt atcaatttct
     5281 ctcaaaagta tctttatgga atagacagtt gtgttaataa agttgtaatt tcaccaaata
     5341 tttatacaga tgaaataaat ataacgcctg tatatgaaac aaataatact tatccagaag
     5401 ttattgtatt agatgcaaat tatataaacg aaaaaataaa tgttaatatc aatgatctat
     5461 ctatacgata tgtatggagt aatgatggta atgattttat tcttatgtca actagtgaag
     5521 aaaataaggt gtcacaagtt aaaataagat tcgttaatgt ttttaaagat aagactttgg
     5581 caaataagct atcttttaac tttagtgata aacaagatgt acctgtaagt gaaataatct
     5641 tatcatttac accttcatat tatgaggatg gattgattgg ctatgatttg ggtctagttt
     5701 ctttatataa tgagaaattt tatattaata actttggaat gatggtatct ggattaatat
     5761 atattaatga ttcattatat tattttaaac caccagtaaa taatttgata actggatttg
     5821 tgactgtagg cgatgataaa tactacttta atccaattaa tggtggagct gcttcaattg
     5881 gagagacaat aattgatgac aaaaattatt atttcaacca aagtggagtg ttacaaacag
     5941 gtgtatttag tacagaagat ggatttaaat attttgcccc agctaataca cttgatgaaa
     6001 acctagaagg agaagcaatt gattttactg gaaaattaat tattgacgaa aatatttatt
     6061 attttgaaga taattataga ggagctgtag aatggaaaga attagatggt gaaatgcact
     6121 attttagccc agaaacaggt aaagctttta aaggtctaaa tcaaataggt gatgataaat
     6181 actatttcaa ttctgatgga gttatgcaaa aaggatttgt tagtataaat gataataaac
     6241 actattttga tgattctggt gttatgaaag taggttacac tgaaatagat ggcaagcatt
     6301 tctactttgc tgaaaacgga gaaatgcaaa taggagtatt taatacagaa gatggattta
     6361 aatattttgc tcatcataat gaagatttag gaaatgaaga aggtgaagaa atctcatatt
     6421 ctggtatatt aaatttcaat aataaaattt actattttga tgattcattt acagctgtag
     6481 ttggatggaa agatttagag gatggttcaa agtattattt tgatgaagat acagcagaag
     6541 catatatagg tttgtcatta ataaatgatg gtcaatatta ttttaatgat gatggaatta
     6601 tgcaagttgg atttgtcact ataaatgata aagtcttcta cttctctgac tctggaatta
     6661 tagaatctgg agtacaaaac atagatgaca attatttcta tatagatgat aatggtatag
     6721 ttcaaattgg tgtatttgat acttcagatg gatataaata ttttgcacct gctaatactg
     6781 taaatgataa tatttacgga caagcagttg aatatagtgg tttagttaga gttggtgaag
     6841 atgtatatta ttttggagaa acatatacaa ttgagactgg atggatatat gatatggaaa
     6901 atgaaagtga taaatattat ttcgatccag aaactaaaaa agcatgcaaa ggtattaatt
     6961 taattgatga tataaaatat tattttgatg agaagggcat aatgagaacg ggtcttatat
     7021 catttgaaaa taataattat tactttaatg agaatggtga aatgcaattt ggttatataa
     7081 atatagaaga taagatgttc tattttggtg aagatggtgt catgcagatt ggagtattta
     7141 atacaccaga tggatttaaa tactttgcac atcaaaatac tttggatgag aattttgagg
     7201 gagaatcaat aaactatact ggttggttag atttagatga aaagagatat tattttacag
     7261 atgaatatat tgcagcaact ggttcagtta ttattgatgg tgaggagtat tattttgatc
     7321 ctgatacagc tcaattagtg attagtgaat agataaaaat atgttaaata tatcctctta
     7381 tacttaaata tataaaaata aacaaaatga tacactacat aaagtgttct atctaatatg
     7441 aagatttacc aataaaaagg tggactatga tgaatgcaca gtagttcacc tttttatatt
     7501 tctaatggta acaaaatatt tttttatata aacctaggag gcgttatgaa tatgacaata
     7561 tcttttttat cagagcatat atttataaag ttagtaattt taactatatc atttgataca
     7621 ttattaggat gtttaagtgc aataaaaagt cgtaaattta attctagttt tggaatagat
     7681 ggaggaatca gaaaagtagc aatgatagca tgtatatttt ttttatcagt agttgacatt
     7741 cttacaaagt ttaacttttt atttatgtta ccacaagatt gtatcaattt tttaagacta
     7801 aaacatcttg gaatatctga atttttctct attttattta ttttatatga aagtgtaagt
     7861 atattaaaaa atatgtgctt atgtggatta ccagtaccta agagattaaa ggaaaaaata
     7921 gcagttttac tagatgcaat gacagatgaa atgaatgcta aggatgaaaa gtaagtaatg
     7981 gtagatataa taaagatatt aacaaataaa aagtgttatc caaataagaa tagctgaaag
     8041 ttatcataat tcatgaaact aataatgaaa acgagggagc agatgccaag agacacacaa
     8101 gtattaaata catataattt cgaagcaagt gttcattact atatggatga caaggtagta
     8161 tatcaaacat tggttcacaa agatggtgca tggtcagttg gtaaaatcta ttaagctaca
     8221 ttagttacag atatcacaaa ctataatagt taaacataga aatatgtgta aattgtgatg
     8281 gaaattattc aaaaacacaa aaatacgtga tgaaggacaa aatgatagag aaaataagta
     8341 tcaaacctta ataaatgatt taattgatag tttaaaagtt ataggaaaaa tatataaaga
     8401 aataaaaaca ttaaaaaaat ataagatatg ttctatcata caatctcctt atctaataga
     8461 agagtcaatt aactaattga gtatctttaa attgaaatgt taggaagtga tttaaatatg
     8521 aaaacttaaa ttataaaaaa tcaatattaa tttattttaa aaaaatagaa aggagtgtat
     8581 aagatttatt ttcaaagttt aaaaacaaga aaatcaattt aaatttcaga aggaataaat
     8641 gtggttatag aagtggattt attatcaaaa ataataatac taggaggttt ttatgtcttt
     8701 aatatctaaa gaagagttaa taaaactcgc atatagcatt agaccaagag aaaatgagta
     8761 taaaactata ctaactaatt tagacgaata taataagtta actacaaaca ataatgaaaa
     8821 taaatattta taattaaaaa aactaaatga atcaattgat gtttttatga ataaatataa
     8881 aaattcaagc agaaatagag cactctctaa tctaaaaaaa gatatattaa aagaagtaat
     8941 tcttattaaa aattccaata caagtcctgt agaaaaaaat ttacattttg tatggatagg
     9001 tggagaagtc agtgatattg ctcttgaata cataaaacaa tgggctgata ttaatgcaga
     9061 atataatatt aaactgtggt atgatagcga agcattctta gtcaatacac taaaaaggct
     9121 atagttgaat cttctaccac tgaagcatta cagctactag aggaagagat tcaaaatcct
     9181 caatttgata atatgaaatt ttacaagaaa aggatggaat ttatatatga tagacaaaaa
     9241 aggtttataa attattataa atctcaaatc aataaaccta cagtacctac aatagatgat
     9301 attataaagt ctcatctagt atctgaatat aatagagatg aaactttatt agaatcatat
     9361 agaacaaatt ctttgagaaa aataaatagt aatcatggga tagatatcag agctaatagt
     9421 ttgtttacag aacaagagtt attaaatatt tatagtcagg atttgttaaa tcgtggaaat
     9481 ttagctgcag catctgacat agtaagatta ttagcctaaa aaattttggc ggagtatatt
     9541 tagatgttga tatgcttcca ggtattcact ctgatttatt taaaacaata tctagaccta
     9601 gctctattgg actagaccgt tgggaaatga taaaattaga ggctattatg aagtataaaa
     9661 aatatataaa taattataca tcagaaaact ttgataaact tgatcaacaa ttaaaagata
     9721 attttaaact cattatagaa agtaaaagtg aaaaatctga gatattttct aaattagaaa
     9781 atttaaatgt atctgatatt gaaattaaaa tagctttcgc tttaggcagt gttataaatc
     9841 aagccttgat atcaaaacaa ggttcatatc ttactaacct agtaatagaa caaataaaaa
     9901 atagatatca atttttaaac caacacctta acccagccat agagtctgat aataacttca
     9961 cagatactac taaaattttt catgattcat tatttaattc agctactgca gaaaactcta
    10021 tgtttttaac aaaaatagca ccatacttac aagtaggttt tatgccagaa gctcgctcca
    10081 caataagttt aagtggtcca ggagcttatg catcagctta ctatgatttc ataaatttac
    10141 aagaaaatac tatagaaaaa actttaaaag catcagattt aatagaattt aaattcccag
    10201 aaaataatct atctcaattg acagaacaag aaataaatag tctatggagc tttgatcaag
    10261 caagtgcaaa atatcaattt gagaaatgtg taagagatta tactggtgga tctctttctg
    10321 aagacaatgg ggtagacttt aataaaaata ctgccctcga caaaaactat ttattaaata
    10381 ataaaatgcc atcaaacaat gtagaagaag ctggaagtaa aaattatgtt cattatatca
    10441 tacagttaca aggagatgat ataagttatg aagcaacatg caatttattt tctaaaaatc
    10501 ctaaaaatag tattattata caacgaaata tgaatgaaag tgcaaaaagt tacttttaag
    10561 tgatgatgga gaatctattt tagaattaaa taaatatagg atacctgaaa gattaaaaaa
    10621 taaggaaaaa gtaaaagtaa cctttattgg acatggtaaa gatgaattca acacaagcga
    10681 atttgctaga ttaagtgtag attcactttc caatgagata agttcatttt tagataccat
    10741 aaaattagat atatcaccta aaaatgtaga agtaaactta cttggatgta atatgtttag
    10801 ttatgatttt aatgttgaag aaacttatcc tggtaagttg ctattaagta ttatggacaa
    10861 aattacttcc actttacctt atgtaaataa aaattctatt actataggag caaatcaata
    10921 tgaagtaaga attaatagtg agggaagaaa agaacttcta gctcactcag gtaaatggat
    10981 aaataaagaa gaagctatta tgagcgattt atctagtaaa gaatacattt tttttgattc
    11041 tatagataat aagctaaaag caaagtccaa gaatattcca ggattagcat caatatcaga
    11101 agatataaaa acattattac ttgatgcaag tgttagtcct gatacaaaat ttattttaaa
    11161 taatcttaag cttaatattg aatcttctat tggtgattac atttattatg aaaaattaga
    11221 gcctgttaaa aatataattc acaattctat agatgattta atagatgagt tcaatctact
    11281 tgaaaatgta tctaatgaat tatatgaatt aaaaaaatta aataatctag atgagaagta
    11341 tttaatatct tttgaagata tctcaaaaaa taattcaact tactctgtaa gatttattaa
    11401 caaaagtaat ggtgagtcaa tttatgtaga aacagaaaaa gaaatttttt caaaatatag
    11461 cgaacatatt acaaaagaaa taagtactat aaagaatagt ataattacag atgttaatag
    11521 taatttattg gataatatac agttagatca tacttctcaa gttaatacat taaacgcagc
    11581 attctttatt caatcattaa tagattatag tagcaataaa gatgtactga atgatttaag
    11641 tacctcagtt aaggttcaac tttatgctca actatttagt acaggtttaa atactatata
    11701 tgactctatc caattagtaa atttaatatc aaatgcagta aatgatacta taaatgtact
    11761 acctacaata acagagggga tacctattgt atctactata ttagacggaa taaacttagg
    11821 tgcagcaatt aaggaattac tagacgaaca tgacccatta ctaaaaaaag aactagaagc
    11881 taaggtgggt gttttagcaa taaatatgtc attatctata gctgcaacgg tagcttcaat
    11941 tgttggaata ggtgctgaag ttactatttt cttattacct atagctggta tatctgcagg
    12001 aatactttca ttagttaata atgaattaat attgcatgat aaggcaactt cagtggtaaa
    12061 ctattttaat catttgtctg aatctaaaaa atatggccct cttaaaacag aagatgataa
    12121 aattttagtt cctattgatg atttagtaat atcagaaata gattttaata ataattcgat
    12181 aaaactagga acatgtaata tattagcaat ggagggggga tcaggacaca cagtgactgg
    12241 taatatagat cactttttct catctccatc tataagttct catattcctt cattatcaat
    12301 ttattctgca ataggtatag aaacagaaaa tctagatttt tcaaaaaaaa taatgatgtt
    12361 acctaatgct ccttcaagag tgttttggtg ggaaactgga gcagttccag gtttaagatc
    12421 attgggaaat gatggaacta gattacttga ttcaataaga gatttatacc caggcaaatt
    12481 ttactggaga ttctatgctt ttttcgatta tgcaataact acattaaaac cagtttatga
    12541 agacactaat attaaaatta aactagataa agatactaga aacttcataa tgccaactat
    12601 aactactaac gaaattagaa acaaattatc ttattcattt gatggagcag gaggaactta
    12661 ctctttatta ttatcttcat atccaatatc aacgaatata aatttatcta aatatgattt
    12721 atggatattt aatattgata atgaagtaag agaaatatct atagaaaatg gtactattaa
    12781 aaaaggaaag ttaataaaag atgttttaag taaaattgat ataaataaaa ataaacttat
    12841 tataggcaat caaacaatag atttttcagg cgatatagat aataaagaca gatatatatt
    12901 cttgacttgt gagttagatg ataaaattag tttaataata gaaataaatc ttgttgcaaa
    12961 gtcttatagt ttgttattgt ctggagataa aaattatttg atatccaatt tatctaatac
    13021 tattgagaaa ataaatactt taggcctaga tagtaaaaaa tatagtgtac aattacactg
    13081 atgaatctaa taataaatat tttggagcta tatctaaaac aagtcaaaaa agcataatac
    13141 attataaaaa agacagtaaa aatatattag aattttataa tggcagtaca ttagaattta
    13201 acagtaaaga ttttattgct gaagatataa atgtatttat gaaagatgat attaatacta
    13261 taacaggaaa atactatgtt gataataata ctgataaaag tatagatttc tctatttctt
    13321 tagttaataa aaatcaagta aaagtgaatg gattatattt aaatgaatcc gtatactcat
    13381 cttaccttga ttttgtgaaa aattcagatg gacaccataa tacttctaat tttatgaatt
    13441 tatttttgga taatataagt ttctggaaat tgtttgggtt tgaaaatata aattttgtaa
    13501 tcgataaata ctttaccctt gttggtaaaa ctaatcttgg atatgtagaa tttatttgtg
    13561 acaataataa aaatatagat atatattttg gtgaatggaa aacatcgtca tctaaaagca
    13621 ctatatttag cggaaatggt agaaatgttg tagtagagcc tatatacaat cctgatacgg
    13681 gtgaagatat atctacttca ctagattttt cttatgaacc tctctatgga atagatagat
    13741 atatcaataa agtattgata gcacctgatt tatatacaag tttaataaat attaatacca
    13801 attattattc aaatgagtac tatcctgaga ttatagttct taacccaaat acattccaca
    13861 aaaaagtaaa tataaattta gatagttctt cttttgagta taaatggtct acagaaggaa
    13921 gtgactttat tttagttaga tacttagaag aaagtaataa aaaaatatta caaaaaataa
    13981 gaatcaaagg tatcttatct aatactaaat catttaataa aatgagtata gattttaaag
    14041 atattaaaaa gctatcatta ggatatataa tgagtaattt taaatcattt aattctgaaa
    14101 atgaattaga tagagatcat ttaggattta aaataataga taataaaact tattactatg
    14161 atgaagctag taaattagtt aaaggattaa tcaatataaa taattcatta ttctattttg
    14221 atcctataga atctaactta gtaactggat ggcaaactat caatggtaaa aaatattatt
    14281 ttgatataaa tactggagca gcttcaacta gttataaaat tattaatggt aaacactttt
    14341 attttaataa taatggtgtg atgcagttag gagtatttaa aggacctgat ggatttgagt
    14401 attttgcacc tgccaatact cagaataata acatagaagg tcaggctata gtttatcaaa
    14461 gtaaattctt aactttgaat ggcaaaaaat attattttga taatgactca aaagcagtta
    14521 ctggatggca aaccattgat ggtaaaaaat attactttaa tcttaacact gctgaagcag
    14581 ctactggatg gcaaactatt gatggtaaaa aatattactt taatactaac acttccatag
    14641 cttcaactgg ttatacaatt attaatggta aacattttta ttttaatact gatggtatta
    14701 tgcagatagg agtgtttaaa ggacctaatg gatttgaata ctttgcacct gctaatacgg
    14761 atgctaacaa tatagaaggt caagctatac gttatcaaaa tagattccta tatttacatg
    14821 acaatatata ttactttggt aataattcaa aagcagttac tggatggcaa actattaatg
    14881 gtaatgtata ttactttatg cctgatactg ctatggctgc agctggtgga cttttcgaga
    14941 ttgatggtgt tatatatttc tttggtgttg atggagtaaa agcccctggg atatatggct
    15001 aaaatatatg tgtgacaaaa aattattcct gtgctactaa gaaattattt ttatataata
    15061 aatattgaga tttaattaaa agtcatgtgt tattgtaata catgactttt agttaaaatt
    15121 tttctatcat ttaataatct attattcttg actattttat aataaaattc gtatatggaa
    15181 atattaatac taaataatta atagttgata gaaaatagat aatgtgctaa aagcaaaaac
    15241 taatttagag ccttgtaact gtttatttgc aattataaaa acatctttaa acatattgac
    15301 tataatataa aatattaact ataatacaaa acaatattaa ttaattttct ctacagctat
    15361 ccctggtata gttatatttc cacccatagt tgattcataa gttatagttc cagcacttat
    15421 acctcttata gttatgtaat catcctctaa tactcttgaa ggagttattg attttttata
    15481 actacatagt acaaccttat cataatttcc agacacagct aatcttattt gcacctcatc
    15541 accatcttca ataacttgaa taaccttacc ttcaaatttt acctttttgt acttataatc
    15601 atcaggtgtt ctagctaatt ggtcataagt aataccagta tcatatcctt tcttctcttc
    15661 ttcttctttt ttacgttgtt cttcagcctt tttagcttct tcagcttttt tagcttcttc
    15721 agctttacgt tgattttcag cttcaatagc tttcttttcg tcgtctttca ttttgaacca
    15781 tggttcagca tcagacaatt tgctatttaa agtttctatt tctgtttttt gagaatctat
    15841 aactgatgtt tgattagaaa tgaccttctc atggtcttca gaacaagctg gtgaggatat
    15901 attgccaaaa aacacgccta aaataaatac cagtagagca ataccctttg tgctcttaaa
    15961 gaatttaatt attttcttag agcttccttt tccttcatta ctaaattcgt taccctcatt
    16021 ttttttagaa aacataatac aatctccctt ttattagata tattttattt taaaagttaa
    16081 aaacttaaaa taactagttg actaaaaatt atatattcat attttcctaa aatatatttc
    16141 ccctaagcat aattttacaa aataaagtaa gaaaattcaa tgtattttga aaaataaggt
    16201 aaaaatgtca gattataata taattttctt gaaggtgaat tttatatatt aaatgaaaca
    16261 taaagtattt tatttgtata aaactatatt atgatgtaat taagtgactc agtactttag
    16321 aggtggtaaa atatacatag atgaaagagc ataaataaat tgatataata aaaattaaat
    16381 attaactata ataaaaaaat atctggtact aagtataata caaaaataat atgccagaaa
    16441 gaaggcgaag tatgggagaa ctacaaaaaa ttccaggagt agggaaagca acagaaaaat
    16501 ctttaatcat gttaggatat acaactatta aatcacttaa agatgcaaac ccagcacaaa
    16561 tgtatgaaaa ggaatgttta atgcgtggac aacatataga cagatgtcag ttatatgtat
    16621 acagatgtgc agtatatttt gcatcaactg aaaatccaga accagaaaaa ttaaagtggt
    16681 ggtattggaa agattaaaaa atcaaaaaag ttagatataa aaaagagttg aatatccgtt
    16741 ttaaataata aaaactaata ctcaactctt ttttagtcgt aaattttatg taaatctatt
    16801 tgtatggcta aatataccca tcaataaatg tataaatata ccaatcaaca aatgactaaa
    16861 catactatct aacaaagtat taaataaatt gcttaacaaa acattctaaa acaattaatt
    16921 agaaactgtt tcttcaagcc attttctgca ttgctcaata tttaccatag ggtcaaaacc
    16981 ttggtctttt cccttattta aaagcatatc acaagggaaa gtatcacatt caccacaatg
    17041 attaagttcc ttactttcac agcaagtttt aaccttacat tctccacccc aaaatggttt
    17101 ttccatagtt gggcaaccag tgcaatttac ttgttctttt tttgtacaac tgtcacaaca
    17161 gacaccacat cttgattcaa acatattcaa acatcctttc cattatttaa aaatattata
    17221 aaataatttt agatgactac aaaagtgcaa ataattgatt ttaagtcatc taaacattaa
    17281 acataaaatt attagtactg gtaaatttat aaaaactatt ccaaaaatat ttttgaatat
    17341 ttcaaagcac aataaattac ttcaaatcca tttgaatatt tagtaaaact atagattgat
    17401 ttaaatctct ttgaatattc cataacatca tagattgctt taaatctctt tgaatattcc
    17461 ataacaccat agattacttt aaatcccttt aaatattcca taacacaata aattacttta
    17521 aatctctttg aatattccat aacacaataa attactttaa atctctttga atattccaca
    17581 agactataga ttactttaaa tccctttgaa tattccataa cactataaat tacttttagc
    17641 ttttatataa ccataaagtt tttgtctgga actttaaatg gagttttata tcaaatttgc
    17701 attccatcaa ataaccaaac ttaatttaat tacacatttc aagggtcaaa tatttattca
    17761 atatatttga taattctatg tt
//
LOCUS       DAEPXF010000075        17696 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain HB27
            SAMN13376389-rid12479423.denovo.079, whole genome shotgun sequence.
ACCESSION   DAEPXF010000075 DAEPXF010000000
VERSION     DAEPXF010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13376389
            Sequence Read Archive: SRR10518432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 17696)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17696)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001069624.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001069624.1
            Genome Coverage       :: 315x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/15/2021 01:54:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,165
            CDSs (total)                      :: 4,076
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 89
            rRNAs                             :: 10, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 10 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 19 of 69
            Pseudo Genes (incomplete)         :: 44 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 10 of 69
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17696
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13376389-rid12479423.denovo.079"
                     /strain="HB27"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="China: Hebei"
                     /collection_date="2017"
     gene            complement(88..1302)
                     /gene="dpaL"
                     /locus_tag="KRL67_002141"
     CDS             complement(88..1302)
                     /gene="dpaL"
                     /locus_tag="KRL67_002141"
                     /EC_number="4.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopropionate ammonia-lyase"
                     /protein_id="HBH1523015.1"
                     /translation="MREIKWKLNNLPKSEEKEKGIEFLKDEEIAKAKAFHESFPQYEK
                     TPLVKLDNLAKSLGVSGIYVKDESYRFGLNAFKVLGGSYSMGRYLAQRLNTDISELGY
                     DKLTSKEIKEKLGEITFFTATDGNHGRGVAWTANKLGQKSVVLMPKGSSEFRLNKIKG
                     EGADASITDLNYDDAVRLANDYAEADDHGVMVQDTAWDGYEEIPAWIMQGYGTMAKEA
                     IEQLKEYGVDRPTHVFVQAGVGSLAGAVQGYVASIYDECPITVVVEADEADCYYKSAE
                     AGDGKPRFVGGDMPTIMAGLACGEPNTIGFEVLKNYSSAFVSAPDWVSAKGMRILGNP
                     LRGDEKVISGESGAVTTGALVSILGSEDLKDLREALKLDENSKVLLISTEGDTDPDKY
                     RDIVWNGDCQSK"
     gene            complement(1907..2446)
                     /locus_tag="KRL67_002142"
     CDS             complement(1907..2446)
                     /locus_tag="KRL67_002142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2'-5' RNA ligase family protein"
                     /protein_id="HBH1523016.1"
                     /translation="MQYAIELYYDKKTEKQLFDLSKKIADEKISTKYLEWKTRPHLTL
                     ACFNDVDEACCIDKLKGFAQNHKVIPAYIGSVGMFNDTKTVFVSPIMNSSMYQFQREL
                     YEVLQDFDSNGWEWYHPNRWVPHCTIALTGDDESEAFFRASDVLLREFKKISGEFVSI
                     GLVKITFPVEEIFTVNMNR"
     gene            complement(2522..4327)
                     /locus_tag="KRL67_002143"
     CDS             complement(2522..4327)
                     /locus_tag="KRL67_002143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBH1523017.1"
                     /translation="MLSKLKEFQQEMIKYTETVASVLDVDIEIVDDRLIRISGTGLYK
                     SKINESVVTEGFIYDNVIQTGQELVVLDICDNQLCIECSHYMKCLNKVIIAVPIKYNN
                     RTIGVIGAISTDKTKKVEISAKIDNYLKFVNHICDLISMKIEEHEASKNSSRKMDMMI
                     EIIENVEKGVIILDINSKISYINNIALKKLDIDKNIIENIVNIVSVESSSNGHELLEI
                     DIDNKIYNINAKIIPVYPYINQYDKIIIFDKTYINHKGHGKVNSGWGNSDIESIIGNS
                     ESMLKVKERTKKLAKSNSTVLITGESGTGKELIARAIHAEGSRWNKPFIAINCAAIPE
                     NLLESELFGYIKGAFSGASSGGKVGKFELANEGVIFLDEIGDLSMPLQAKLLRVLQER
                     KFARIGSNKLIDLDIRVIAATNKNLLKLVNEGKFRDDLYYRLNVIPINLPPLRERKDD
                     IEAIMMKFASKYSLELGIQLSKIEENVMNMLINYNWPGNIRELENAVEYMMNLVGDDG
                     IIYKDMLPLDILNYYNINGNVCKNKDINIIFEDDIVGGIVENQERILSIKELELTYIN
                     KLLNKYGRDTKTKKKIAKDLGIGLATLYRKLEEEQ"
     gene            complement(4723..5100)
                     /locus_tag="KRL67_002144"
     CDS             complement(4723..5100)
                     /locus_tag="KRL67_002144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003483933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HBH1523018.1"
                     /translation="MSKVVIATEKAPGAIGPYSQAIQVGNIVSTSGQLPIDMKTGELV
                     SDPKEATKASLDNVKAILEQAGTSMDKIFKTTVFVKDLKDFNDINEVYATYFKENPPA
                     RSCVQVAQLPKDAVLEIEAMAVL"
     gene            6004..6489
                     /locus_tag="KRL67_002145"
     CDS             6004..6489
                     /locus_tag="KRL67_002145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH1523019.1"
                     /translation="MSKSELTAETTEEMLEVLSGKDYDIACHLHELGKSLDCKIEPKT
                     GARSYKIVYSTKKPKRSLFTIECNEKKWRVKANLFHLNTYKDAVEECSKTIKDSITKT
                     RTCKKCNSKCIGGSSFELDGKFYLTCIGSGHYFANMEEIDWKNLEKLITKENNIMQES
                     V"
     gene            complement(6689..7228)
                     /locus_tag="KRL67_002146"
     CDS             complement(6689..7228)
                     /locus_tag="KRL67_002146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002585630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HBH1523020.1"
                     /translation="MSKNVLIVSASPRKGGNSDLLCDQFIQGALESKNQVEKIFLKDK
                     DINYCTGCGACYEKEASCSQTDDMGEILEKMIEADVIVLATPVYFYTMNAQMKTMIDR
                     TCSRYTEICDKDFYFIVSAADENVADMERTIEGFRGFTSCLNGSKEKGIVYGVGAWNV
                     GDIKGSKAMKQAYEMGKLV"
     gene            complement(7221..7634)
                     /locus_tag="KRL67_002147"
     CDS             complement(7221..7634)
                     /locus_tag="KRL67_002147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HBH1523021.1"
                     /translation="MTISEVSKKYELSADTLRYYERIGLIPPVNRNKSGIRSFTEEDC
                     EWVNFIKCMRGAGLSIETLIEYVAMFQQGSSTIKARKELLIEQRNQLAKRIEEMQKTL
                     ERLNFKIDRYEEGIIEKEKVLKSSRNKKMEEVSYE"
     gene            complement(7670..8791)
                     /locus_tag="KRL67_002148"
     CDS             complement(7670..8791)
                     /locus_tag="KRL67_002148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HBH1523022.1"
                     /translation="MEAKKLGFGLMRLPIKDENDVTTIDMEHLNKMVDTFLERGFTYF
                     DTAYVYHMGKSEIALRESLVKRHKRESFTVATKLPLMALKKKEEQETIFNEQLEKCGV
                     DYFDYYLLHNIGVSHYEVAKKFDSFKFIEEKKKEGKIKNIGFSFHDSAELLDKVLSEH
                     PEVDFVQLQINYLDWDNESIQSRKCYEVAEKHNKPVIVMEPVKGGTLAKIPEKAEKLL
                     NDYNPDMSISSWAIRFAASKDNVMMVLSGMSNMEQLLDNTGYMQNFKPFVQEEYNIID
                     EAVEIINESILVPCTACQYCVEGCPKNIAIPNYFALYNAEKQALNTGFSTQMVYYNNY
                     TKTYGKASDCIECKQCEESCPQHIKIIDALKNVAEIFEK"
     gene            9390..11051
                     /locus_tag="KRL67_002149"
     CDS             9390..11051
                     /locus_tag="KRL67_002149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp21"
                     /protein_id="HBH1523023.1"
                     /translation="MNKKNLAILISGMMFFSSSSAIFADNTTKQERLIGKTRYETAVE
                     VSKLGWVQSKTAIIVNGNSIQSALCANPFAKLKNAPILLVNNNSIENSTKSELKRLGV
                     DNVYIVDSGNSISSKVENEIKSLNIKINKIVGNNIYEMSTNVLKEIDKIKKIENVAIV
                     NGLKGLSDAVSIASPAAIHNMPIILVSDENSNDSSINFLKNRSIKKSYIVGGNRILSD
                     NAVKKYPNRERISGSDRNNTNAKIIDKFYKDKKINNVFVAKDGINQENALVDSLSIGV
                     LAAKENSPIMISNGSLVNSQKEFIKNKDIDEITQVGGGYNQKAFEDILKLKNVNIRNK
                     TKKISLIQAKKILKQALPGSNILVCYYDEDDNKYDCKVIKDNVLYEIEIDAYSGKIIE
                     IEKDDDLNDNNLGTQNISLDKAKSIALDAVPNGNLIYCKYDAEDNEYEAKVIKNKKTY
                     ELEISAFDGRIVDIDMDKDSDDDFDDNDNNHNNSSNTNDSNSIIGAEKAKSIMLDKVH
                     GGKIVKFEYDEDDKEYEGEIIKGKTKYEITINAITGEILEFEIDD"
     gene            11348..12094
                     /locus_tag="KRL67_002150"
     CDS             11348..12094
                     /locus_tag="KRL67_002150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005340256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBH1523024.1"
                     /translation="MNTILKVNSITKTYKGQQPSLDKVSFNVSEGEFIGIMGASGSGK
                     TTLLNVLSTIDKPTSGDIFINNINLKSLNDTSAADFRKDNLGFIFQEYFLLDSLTIFE
                     NISVPLTLQKLSPDKIEKMVKSLAERFGIASQLNKYPNELSGGQRQRASAVRAIVKHP
                     SILFADEPTGALDSNSATELLHSLQEANEELKTTILMVTHDVYAASFANRILIFKDGH
                     IIKELNKQQNNRHTFYELIIQEIAKLDTKR"
     gene            12107..14134
                     /locus_tag="KRL67_002151"
     CDS             12107..14134
                     /locus_tag="KRL67_002151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBH1523025.1"
                     /translation="MNIFYMAIKNLKKNFSFYSLYLLSVSFVITVFFAFTSFSVNKIM
                     LEKISTDGRVESMCNLISIFLMAFVIFYMAYSNRFFLRRRTKELGIYALLGYRKFTIL
                     SLLTSENILICSGAFIIGLVLGALAHKGIVFGITALLNLSIDNSQIPFFNINAINKTI
                     IFIALVVLVLAISNGKFLYKTSLIKLIRYEKSTEKKLKFRKIPAFMGFIMILSGYSLS
                     LDILRGAKSVWISIGFYQMGLITMIIIVIGTVLFINSFLPYIMQKSKKNKRAFYTGTK
                     IITIPNFIYRIRSNSKTLIMLTLLSAATLTVSSVMALSLYYPIASVSRIAPSEIEFRV
                     EKNKQIEDVKQIVSQYMSNTDDIIFTQTNIYKVKSSSKDLPLEYEIGTSKNNLSNRNS
                     SKEVISRESGFECISFSDYYTLLKAQNRTKLIKQLIPLRDNECILMKYQPNNDRYSEN
                     GTIYPLLINGKETPVTVRETSLNNAISFANSVGTLIVNDSIYEQIAQNKLPMTSILSI
                     NGKSIKNNENLYSNISDYLDGSPYLQGYSHRINDLLSINSSTFLLIGFLVVLFFIASG
                     SILYFNNISAISDSTSDYKILQKMGYTNIQIKKIIKKQVLPFFNIPFLLGIVDCIFAT
                     LVYKTALMQNLLGNSLSQYIPVIIAIFLTSVVYLIYYILTIKSCCKIVLKK"
     gene            14278..15861
                     /locus_tag="KRL67_002152"
     CDS             14278..15861
                     /locus_tag="KRL67_002152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBH1523026.1"
                     /translation="MTNFNLKILFLGNYILSLLFMGISTSTETYMNIQSIIPLIISTL
                     LLIDITYITSCNVRENNIISLFCGLLALDSWYLLLSFVATPIGTIIFKILSTVIAYVS
                     IKFIFLFLFQGSGYKFRKITDILLLITCIGAIVGAFISNRTYACMYGIQFVVSIISFF
                     YIIFYHWKRVAFVIKSEKKPISISVFITIIGFLTYYFFTLRISNHIGNFGIYLTVLVF
                     FMSIHGIALKENDSFPISTVFNIKQRSIFLFISILVISLVVLFASGNFYIILIAINVL
                     FMLIFLCNIILEFNLRNEKNKVVKNSKYTFALEKLQQEEQLKTEFSNFLHDDILQNLL
                     SIKNMMSKSYRPEVQDIIYETLNNLNTCIRKQMQDYHPVILKNLTVKENYQNLIESVS
                     SSFPQKNICVSFKCSDTLFLVEPYDILIYRLIKELLTNVYKHSNGSHAWIALALEKNI
                     IKLNINDNGVKNTSFKTPLDITKITFDTIKHKGIISIKEQVENLGGTISISDNIPHGV
                     CVQITIPMKGDGSYKYFVS"
     gene            15833..16453
                     /locus_tag="KRL67_002153"
     CDS             15833..16453
                     /locus_tag="KRL67_002153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBH1523027.1"
                     /translation="MVLINILLVDDHMLFAKSLSIALEEYEEIEQFYSTQDIHSLSEM
                     IQDKKIDIVLMDINLRGLSECDGLKVAYNLLETNSTIKIIILTGYDLPVYKHEAKKIG
                     IYGFLNKNISPDNLIDSLLKVYQGGTCFPSENTEVIIEDLTSMEKRILQLICSGKKRK
                     IIADELYISERTLSNHLQHIYDKLDVSSSVEAITKAIQMGYISPVY"
     gene            complement(16653..17579)
                     /locus_tag="KRL67_002154"
     CDS             complement(16653..17579)
                     /locus_tag="KRL67_002154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conserved phage C-terminal domain-containing
                     protein"
                     /protein_id="HBH1523028.1"
                     /translation="MSNSFYQLNSAVLYFNEKRDINRIRFNHYVMRKENIQRLKGTLP
                     RGQFMMTVRKAASDLDVSISTISRLVNEFIDLGILRLISRGVKGNSCSVYSYVCSENS
                     NAEIVAKNGDIFKNIEKYIDVTINNNQKTMKYKHRDELFKNSNTNIYSRISEESIDKD
                     YDLIIHTGYETENETKKKELLKNNLNKKNLNKNTFENSDEDLTEDSTYKNPESIHEII
                     IRKLNSESGKNFKADLSITKALIDKRLKEGYSLEDFYKVIEAKVRRWRYTDMEIYIEP
                     TILFGYEFESYLSEESSLSACNYGDMCYEDMY"
BASE COUNT         6143 a         2776 c         2136 g         6641 t
ORIGIN      
        1 atttaatttt atttagtttt tattttgaat ttttcatttg ctttagtttt tcttttattt
       61 gtttagtttt tcttttcctt aagtctttta tttactttgg cagtcaccat tccaaactat
      121 atctctatat ttatcagggt cagtatctcc ttctgtactt attaataata ccttagagtt
      181 ctcatctagt ttcaaagctt ctcttaagtc tttcaaatct tcacttccaa ggattgaaac
      241 taatgctcca gttgtaactg ctccagattc tccagatatt actttctcat ctcctcttag
      301 aggatttcct aatattctca tacctttagc agacacccag tctggtgctg aaacgaatgc
      361 tgatgaataa ttttttaaca cttcaaaccc tatagtattt ggctctccac aagcaagccc
      421 tgccattata gttggcatat ctcctccaac aaatcttggc tttccatctc cagcttctgc
      481 tgatttatag taacaatcag cttcatcagc ctctactact actgttattg gacattcatc
      541 atatatcgaa gcaacatacc cttgaactgc accagcaagt gaaccaactc ctgcttgcac
      601 gaatacatgt gttggtctat ctactccata ttcttttaat tgctctatag cttctttagc
      661 catagttcca tatccttgca ttatccatgc tggtatttct tcatatccat cccatgctgt
      721 atcttgaacc attactccgt ggtcatcagc ttctgcataa tcattagcta atcttactgc
      781 atcatcgtag tttaagtctg ttatactagc atctgcacct tctcctttga ttttattcaa
      841 tctaaattca gaagaacctt ttggcattaa tacaacagat ttttgaccta atttattagc
      901 tgtccaagca actcctctac catgatttcc atctgtggct gtaaagaaag ttatttctcc
      961 taatttttct tttatttctt ttgaagttaa tttatcatac cctaactcag atatatctgt
     1021 atttaatctt tgagctaagt atctacccat agagtaagaa ccacctaata ctttaaatgc
     1081 gtttaagcca aatctataag attcgtcttt aacatatatt ccgcttacgc ccagtgattt
     1141 tgctaagtta tctaatttaa ctaaaggagt cttttcatat tgtggaaagc tttcatgaaa
     1201 agctttagcc tttgcaatct cttcatcttt taaaaattcg attccttttt ctttttcctc
     1261 tgatttaggt aaattattta atttccattt tatttctctc atttatttta tattcccctt
     1321 tcaaattttt cgatgtcatt ttaatttttt atctttgtgt cttatagtga agcaattact
     1381 atgccaatat tatttattct aaaaacaatt tttttatgtt ggaattacat atatttataa
     1441 aatttctttg aattttataa atattaaatg tgaaaaataa gatttataaa agaaaaaatt
     1501 ctcatattgg gaaattttta tcaatacgag aattatcaat tcgagaaatc gtttatttat
     1561 tttaattctt cctttattat gctcttattt tttatatgtc tattgttttg tcagatatat
     1621 tgctactttt attttctata aatatatggc tataaccttc ttacgtataa actctataaa
     1681 tttatatact ttatattctt tattacccaa agcctccaaa atggcttaaa tcttttcata
     1741 gcttcttaca gaagtttaca tgggcgtccc cccaataagc aaaagtgcaa tatgtgtacc
     1801 attgtgccct acttgcgact attgagctgt cgaaattcgc aacttcgcat ttatctgatg
     1861 aaatgcacta tgcaaaatat tcataatatc cacaacagag ttaaagttac ctattcatat
     1921 tcactgtaaa aatttcttcc acaggaaatg taattttaac caatcctatt gagacaaact
     1981 caccacttat ttttttgaac tcacgcagta atacatcact tgcccgaaaa aaagcctcac
     2041 tttcatcatc tccagtaagt gctattgtgc aatggggtac ccaacgattg gggtggtacc
     2101 attcccaacc atttgaatca aaatcttgta gtacctcata taattctctc tgaaactgat
     2161 acatactact attcattata ggtgatacaa acaccgtttt agtatcatta aacatgccta
     2221 ctgaaccaat gtaagcaggt attaccttat ggttttgtgc aaaccccttt agcttgtcta
     2281 tgcaacaagc ctcatccaca tcattgaaac aagccaaagt aagatgaggt cttgttttcc
     2341 attctaagta cttcgtacta attttttcat cagcgatttt tttagataaa tcaaaaagtt
     2401 gcttttccgt ttttttatca taatatagct ctatcgcata ttgcataata acacctccac
     2461 cataaaataa aaatacctat ctataaaaca cacaaatacc tatcatttta atattatatt
     2521 actactgttc ctcttccaat tttctatata gtgtagccaa acctatgcct aagtcttttg
     2581 ctatcttctt cttcgtcttt gtatctcttc catacttatt caacaacttg tttatgtaag
     2641 tcaactctaa ttctttaatt gaaagtatcc tttcttgatt ctctactatc cctccaacta
     2701 tgtcatcttc aaatattata tttatatcct tatttttaca aacatttcca ttaatattat
     2761 aataatttaa aatatccaat ggaagcatat ctttatatat tattccatca tctccaacca
     2821 aattcatcat atattcaact gcattttcca gttcccttat atttcccggc caattatagt
     2881 taatcaacat attcattaca ttttcttcta ttttacttaa ctgaatacct aattctaaag
     2941 aatatttaga agcaaacttc atcattattg cctcaatatc atcctttctt tccctaagag
     3001 gtggcaaatt tataggaatt acatttaatc tatagtataa atcatcccta aatttacctt
     3061 catttactaa tttcaataag tttttatttg tagcagcaat cactcttata tctaaatcta
     3121 taagtttatt tgacccaatt ctcgcaaatt ttctctcttg taaaactcta agtaactttg
     3181 cttgtaaagg cattgacaaa tctcctattt catcaagaaa tataacacct tcatttgcaa
     3241 gttcaaattt acctacttta cctccactac tagctccact aaatgcacct tttatatagc
     3301 caaatagttc actttcaagg agattttctg gaatagcagc acagtttatc gcaataaatg
     3361 gcttgttcca tctactacct tcagcatgta tagctctagc aattagctct ttacccgtac
     3421 cactttcccc agttataagt acagttgaat ttgactttgc taatttctta gttctctctt
     3481 ttacttttaa cattgactct gaatttccaa ttatagactc tatatcacta tttccccagc
     3541 cactattgac ttttccatgt cctttatgat tgatatatgt cttgtcaaat attattattt
     3601 tgtcgtattg atttatatat ggatataccg gaattatttt ggcatttata ttataaattt
     3661 tgttatcaat atcaatttct agtaattcat gtccatttga ggaagattca acagatacta
     3721 tatttactat attttctatt atattcttat ctatatctaa tttttttaga gctatattgt
     3781 ttatataaga tattttgcta tttatgtcta gtattataac gcctttttca acattttcaa
     3841 ttatttctat catcatatcc atttttctac tgctattttt tgacgcttca tgttcttcta
     3901 tcttcataga aattaaatcg catatatgat tgacaaattt taaataatta tctatttttg
     3961 cacttatttc tactttttta gttttatctg tactaatagc accaattact cctatagttc
     4021 gattattata ctttattgga actgctatta ttactttatt taggcatttc atgtaatggg
     4081 aacattctat acaaagctga ttatcacata tatccaatac gactaattcc tgacctgtct
     4141 gtataacatt gtcataaata aatccttctg taacaacgct ttcatttatc tttgatttat
     4201 acagacctgt gccactaatc ctgattaatc tatcatcaac tatttcaata tctacatcta
     4261 acacactagc tactgtttca gtgtatttta tcatttcttg ttgaaattct tttaatttac
     4321 ttaacatagt ttcccccatt agaatttaat ttaccaaatt atcagtttag ctatatttta
     4381 acatatagtc actctttaaa atatatataa aacattactt atttatatat ttgcgttaaa
     4441 acacacaata ttaatttata aaaagtttgt tttatatagt gaccttaatt ataatatgca
     4501 gacaataaaa taaatcacaa tacaataata aaaaaaagct ttcctcatat aacaaatata
     4561 tttttgacat aaaaatatac attatatgag aaaagctcaa tagttaaata tttagataga
     4621 tttttatata gactttttta atataaactt ttttaattgt agactttttt tattagtttt
     4681 taattttcat tagttttgtt attggtttta ttgttataat acttataata ctgccattgc
     4741 ttcaatttct aaaactgcat cttttggtaa ttgagcaact tgtacacatg accttgctgg
     4801 tggattttct ttaaaatatg tagcatacac ttcattaata tcattaaaat cctttaaatc
     4861 ttttacgaat actgtagttt taaatatttt atccatactt gtaccagctt gttctaatat
     4921 tgcttttaca ttatctaaag aagcttttgt tgcttctttt gggtcagata ccaactcgcc
     4981 agtcttcata tctattggaa gttgacctga tgtagaaact atgtttccaa cttgaattgc
     5041 ttgagaatat ggtccaattg ctcctggagc tttctctgta gctattacta cttttgacat
     5101 gacttcacct cctattatta agttatattt acaggaaaat aaaatttcct aaacaccaat
     5161 attaatacga acctttagat taaatttcaa atctaaaact tcaaataatc aatacttaga
     5221 tgattatgtt tatttgtgtc ttgttaactt ttattaaagc aattaacatg ccaaacttca
     5281 tttatatata aaattttgtg aagtcattca aaatagttac ctctgaattt tttcgtaaaa
     5341 aaacgctcta aaattacttt ttatgatgtc aatttttttc tcatatctag aaatttacca
     5401 acatgagaat tcttgttttg agaatttaca gattaaagtt attttttaac cttaaattct
     5461 gaacctccca tgactaaagt cacagggttc ctgcttcata gaattttgca taatcaagtt
     5521 attacatatg aaagaactat agttgacaat cccactatta taaataataa aacctttacc
     5581 cataccttat ttaccctagt tatcatagag cctattgaaa atataacagc taaatataat
     5641 aataaccaaa aaattatcaa tatctaccac tgttgctgaa aatccaaaaa actacctttt
     5701 gatagtctta cttaacaatc taattaatct cataataaat ttacaactta taaataaatt
     5761 gttatattgg ccgtatctca acaactttct cctttcctag ttttaaatct tttatagaat
     5821 tgtaccacgt ctaagtaaat tttcatacta ttaagtctta tttctaataa atcattagct
     5881 ctcattctat gcccaataat gtaagattgc agtaatttta ttgattttaa tagttttatt
     5941 taatatactt atattaatta ttttcatatg aaattattat ttatataggg ggattttttt
     6001 aatatgagca aaagtgaatt aacagcagaa acaacagaag aaatgttaga agtactaagt
     6061 ggtaaagatt atgatattgc atgtcattta catgaacttg gtaaatcatt agattgtaaa
     6121 attgaaccaa agacaggtgc tcgttcttac aaaatagtat attcaactaa gaaaccaaaa
     6181 cgtagcttat ttactattga atgtaatgaa aaaaaatgga gagttaaagc aaatcttttt
     6241 catctaaata catataaaga tgctgtggag gaatgctcta aaactattaa agatagtata
     6301 actaaaactc gtacttgtaa aaaatgtaat tcaaagtgta taggaggttc ttcgtttgaa
     6361 ttagatggaa agttttacct gacttgcata ggaagtggtc attattttgc aaatatggaa
     6421 gaaatcgatt ggaaaaacct agaaaaatta attactaaag aaaataatat tatgcaggaa
     6481 tctgtatagt tttttaaggg taattattaa aatatataag taacaacaga tacttaaata
     6541 ggcataaaaa aataaaggat acagtaagga tatagaataa aaataaatga tatagcgtaa
     6601 agaggctgtc tcaaaatata gataagcctc tttttattgt tgaaatgata agaatatatt
     6661 cgattttata caattttata caaacatact acaccaattt acccatttca tatgcttgtt
     6721 tcattgcctt acttccctta atatcaccta cattccaagc ccctacacca tatactatac
     6781 ctttttcctt agaaccattt agacatgatg taaatcctct aaatccttct attgttcttt
     6841 ccatatcagc cacattttca tctgctgctg aaacgataaa atagaaatct ttatcacaaa
     6901 tttcagtata tctagaacaa gttctatcaa tcatagtttt catttgcgca ttcatagtat
     6961 aaaaataaac tggagtcgct aaaacaatca catctgcctc aatcattttt tcaagaattt
     7021 ctcccatgtc atctgtctgt gaacaacttg cttctttttc ataacaagca ccacatccag
     7081 tacaatagtt aatatcttta tcttttaaaa atatcttctc cacttgattt ttagattcta
     7141 atgccccttg tataaactgg tcacacaata agtcagaatt tccacctttt ctaggactag
     7201 ctgatactat taatacattt ttactcataa gacacttcct ccattttctt atttctactg
     7261 ctctttagta ctttttcttt ctcaataata ccttcttcat atctatcaat cttaaaattt
     7321 aatcgttcca aagttttttg catttcttct attctttttg ctagttggtt tctctgctct
     7381 attaaaagtt cttttctagc tttgatagta ctgctccctt gttgaaacat agctacatac
     7441 tcaattaatg tctcaataga aagacctgca cctctcatac actttatgaa attaacccac
     7501 tcacaatctt cttctgtaaa agacctaatt ccacttttat ttctatttac aggtggtatt
     7561 aaaccaattc gttcataata tcgtaaagta tctgctgaaa gctcgtattt tttactaact
     7621 tctgaaatcg tcatacatat cttcccctct tgatattgta tagattgaat tatttctcaa
     7681 agatttctgc tacattcttt aaagcatcta taattttaat gtgttgtgga caactttctt
     7741 cacattgttt acattcaatg caatctgagg cctttccata agttttagta taattattat
     7801 agtaaaccat ctgtgttgaa aatccagtat taagagcttg tttttctgca ttgtacaacg
     7861 caaaatagtt tggaatagcg atattttttg gacagccttc tacacaatac tgacaggcag
     7921 tacaaggaac taaaattgat tcgtttataa tctctactgc ttcatctata atattatatt
     7981 cctcttgtac gaatggtttg aaattttgca tatatcctgt attgtctaat aattgttcca
     8041 tatttgacat cccacttaag accatcataa cattatcttt acttgctgca aaacgtattg
     8101 cccaagatga aattgacata tctggattgt agtcgttcaa taatttctct gctttttctg
     8161 gtatctttgc aagtgtccct ccttttactg gctccataac aattacaggc ttattatgct
     8221 tctctgccac ctcgtagcat tttctagatt gtatgctttc attatcccaa tctaaataat
     8281 taatctgtaa ttgcacgaag tctacttctg gatgctcact caagacttta tccaataatt
     8341 ctgcactatc atgaaaagaa aaaccaatat tctttatttt tccttctttt ttcttttctt
     8401 caataaactt aaagctatca aattttttag ctacttcata atgtgacaca cctatattgt
     8461 gtaaaagata gtaatcaaaa tagtctacac cacatttttc taattgctca ttaaaaatgg
     8521 tttcttgctc ttcttttttc tttaaagcca ttaatggaag ttttgtagct acagtaaaac
     8581 tctctctttt atgtcttttt actagagatt ctctaagtgc tatctcactt tttcccatgt
     8641 gatatacata agctgtatca aaatatgtaa atcctctttc taaaaaagtg tctaccattt
     8701 tatttaaatg ttccatatca atagttgtaa catcattttc atcttttatt ggtaaacgca
     8761 ttaatccaaa tcctaatttt tttgcctcca ttgttttact tcccccttta aaacttgttt
     8821 gttttatttc tctactttaa ctattcttac aagtatataa ttcttgtata attatacttt
     8881 actacttgga gttaacttca agtcaataga tttttttatt tttaagacaa atattataaa
     8941 aatacagtcc tttaatatta atccattgca tgttcctttc acattccaat tctcaaattt
     9001 cacaaactcc aacatccaaa gaaatatata ctttaagaaa ttcagtctat taacattact
     9061 acaaatactt tttagatatg tttaaaaaga aatttcacct aaaagatttg gaaaagattt
     9121 agaaataggt tttgtgccta ttttaaaata tataaaacag tggtaaagac ttatttataa
     9181 gataaaaaat gaatagtgta tacccttttt aaaaagtttt ttcctcttta tatttatgat
     9241 acaatgttag atataaaaag gaaaattttg tattaaagaa atcttattta ttgatttttt
     9301 aactttaaat tcagtatcaa tttgatttta agattatatt ttattaagta tgccaaataa
     9361 caaattttta aagattgtga ggtaaaaata tgaataagaa aaatttagct atattaatat
     9421 cgggtatgat gtttttttct agttcttcag caatttttgc agacaatact acaaagcaag
     9481 aaagattaat aggtaaaaca agatatgaaa cagcagtaga agtaagtaaa ttgggatggg
     9541 ttcaatcaaa aacagccatt atagttaatg gtaactctat tcaaagtgcg ctttgtgcaa
     9601 atccatttgc taagctaaag aacgcaccta tacttttagt aaacaacaac agtatcgaaa
     9661 attcaactaa atctgaactt aaaaggcttg gtgtagataa tgtgtatatt gtagatagtg
     9721 gaaatagtat ttcttctaaa gtagaaaatg aaataaaatc acttaatata aaaataaata
     9781 aaatagtagg aaacaatata tatgaaatgt ctacaaatgt acttaaagaa atagataaga
     9841 taaaaaagat agaaaatgtt gcaattgtaa atggactaaa gggattatct gatgcagtta
     9901 gtatagcttc tccagcagca atacataata tgcctataat attagtatca gatgaaaaca
     9961 gcaatgatag tagtataaat ttcttaaaga ataggtctat aaaaaaatct tacatagttg
    10021 gaggaaatag gattttatct gataatgcag taaaaaaata tccaaacaga gaaagaatca
    10081 gtggttcaga tagaaataat actaatgcaa aaattattga taaattttat aaagataaaa
    10141 aaataaataa tgtatttgta gctaaagatg gtattaacca agaaaatgca ctagtagatt
    10201 cattatcaat aggagtacta gctgctaaag aaaattctcc tataatgata tctaatggta
    10261 gccttgtaaa tagtcaaaag gaatttatta aaaataaaga tatagatgaa ataacacaag
    10321 taggaggagg atataatcaa aaagcgtttg aagatatttt aaaactaaaa aatgtgaaca
    10381 taagaaataa aactaaaaaa atatctttaa tacaggctaa aaaaatatta aagcaagccc
    10441 ttccaggaag taatatttta gtttgttact atgatgaaga tgataataaa tacgactgca
    10501 aagttataaa ggataatgtc ctatatgaga tagaaattga tgcctactct ggtaaaataa
    10561 tagaaattga aaaagatgat gacttaaatg ataataattt aggtacacaa aatatatcat
    10621 tggataaagc taagtctata gcactagatg ctgttcctaa tggtaatcta atctattgta
    10681 aatatgatgc tgaagataat gaatatgagg ctaaagtaat aaaaaataag aaaacatatg
    10741 aattagagat aagtgcattt gatggaagaa ttgtagatat tgatatggac aaggattctg
    10801 atgatgactt tgatgataat gataataatc acaacaatag ctctaatact aatgacagca
    10861 attctattat tggtgctgaa aaagctaaat caataatgct agataaagta catggtggga
    10921 aaatagtaaa atttgaatat gatgaagatg acaaagaata tgaaggtgaa ataataaaag
    10981 gaaaaactaa gtatgagata acaattaatg caattactgg tgaaatacta gaatttgaaa
    11041 tagatgatta ataaaaaaat atattctatt aattattaca aagcaataaa gccataaata
    11101 ttgaaaaatt caatatttat ggctttattt tattttttta atttaaatcc acattatgcc
    11161 attatatatg tctatgcact cttttaaatt aaaatcttgt ccatcatccc aaacctaata
    11221 aagttagatt ttaaacaata tattgtgata taagtaattt gtgtagaatt attgcgtgat
    11281 ttactcatga ttgaacaata aggttaatat ataatttgac tatatcaaga tgaaaggggc
    11341 atatacaatg aatacaatat taaaagtaaa tagcataact aaaacttata aaggacaaca
    11401 gccgagttta gataaggtat cctttaatgt atcagaaggt gaatttatag gtataatggg
    11461 agcttctggt tctgggaaaa caactcttct taatgtatta tctacaattg ataaaccaac
    11521 aagtggagac attttcataa ataatataaa tttgaaatcg cttaatgaca ctagtgctgc
    11581 tgattttcgt aaggataatt taggctttat ctttcaagag tattttttac tagatagcct
    11641 tacaattttc gaaaatattt cagtacctct tacacttcaa aaactaagtc cagataaaat
    11701 agaaaaaatg gtaaagagtc tagctgaacg atttggcata gcttctcaat taaacaaata
    11761 cccaaatgaa ttatctggtg ggcaaagaca gagagcttca gcagttagag caatagtaaa
    11821 acatcctagt attctctttg cagatgaacc aacaggagct ttggattcta attcagctac
    11881 tgaactactc cactcactac aagaagcaaa tgaagagttg aaaacaacaa ttttaatggt
    11941 aacacatgat gtctatgcag caagttttgc aaatagaatt ttgattttta aagatggtca
    12001 tatcattaaa gaattaaata aacaacagaa taataggcat acattctatg agttgattat
    12061 tcaagaaatt gcaaaattgg atactaaaag ataggaggat aatttaatga acatttttta
    12121 tatggcaata aagaatctta aaaaaaactt ttcattctat tctctatatc tactttcagt
    12181 ctcttttgtt attactgtat ttttcgcatt tacttcattt tctgttaata agataatgct
    12241 agaaaaaatt tcaactgatg gtagagttga atctatgtgt aatcttattt cgatattttt
    12301 gatggcattt gtcatcttct atatggcata ctcaaatcga ttcttcttaa gacgtcgtac
    12361 aaaagaatta ggcatatatg ctttacttgg atatcgtaag tttacaatct tatcactact
    12421 tacctctgaa aatatattaa tttgtagtgg agcttttatt attggattgg tattaggagc
    12481 attggcacat aagggtattg tctttggaat tacagcacta cttaatttat caattgataa
    12541 ttcacagata ccatttttta atataaatgc aataaacaaa acaattatct ttatagcatt
    12601 agttgtttta gtcttagcta tatcaaatgg taaatttctt tataagactt cacttataaa
    12661 attgatacga tatgaaaaaa gcacagaaaa aaaattgaag ttcagaaaaa ttccagcctt
    12721 tatggggttt attatgattt taagtgggta ttctttatca ctagatattc ttcgtggagc
    12781 taagtcagta tggataagta ttggatttta tcaaatgggc ttgataacta tgattatcat
    12841 tgtaattggt acagttctct ttatcaattc ctttcttcca tatattatgc aaaagagtaa
    12901 aaaaaataaa cgagctttct atacaggtac aaagataata actattccaa actttatcta
    12961 tcgtatccgt tcaaactcta aaactctaat tatgcttaca ttactctctg ctgctacttt
    13021 gacagtttca agtgtcatgg cactgtcttt atactaccct attgcatccg tttcacgtat
    13081 tgcaccttca gagatagaat ttagagtaga aaaaaataaa caaatagaag atgtaaaaca
    13141 aattgtcagt caatacatgt ctaatacaga cgatattatt tttacacaga caaatattta
    13201 taaggtaaaa tcttcatcaa aagaccttcc acttgaatat gaaattggta catctaagaa
    13261 taatttatct aatcgtaatt cttctaaaga agttatttca agagaatcag gatttgaatg
    13321 tatatctttt tctgattatt atactttgtt aaaggcacaa aatcgaacaa agctaataaa
    13381 acaacttatc cctttaaggg ataatgaatg tattttaatg aaatatcaac caaacaatga
    13441 tagatatagt gaaaatggaa cgatttatcc attacttatc aatggtaaag aaacacctgt
    13501 aactgtaagg gaaacatcat taaacaatgc tatttctttt gctaatagcg ttggaacact
    13561 tatcgttaat gatagtatat atgagcaaat agcacaaaat aaattaccaa tgacaagcat
    13621 tttaagtatt aatggtaaat caattaaaaa taatgaaaat ttatatagta atatttcaga
    13681 ttatttagat ggaagccctt atttacaagg atattctcac aggataaatg atttattatc
    13741 aattaatagt tctacatttc tattaatcgg atttcttgta gtattattct ttattgcttc
    13801 tggaagtatt ttatacttta acaatatatc agcaatttca gattcaacat ctgattataa
    13861 aattttacag aaaatgggtt atactaatat acaaattaaa aaaattatta aaaaacaagt
    13921 attgccattt tttaatattc cgtttttact tggtatagtg gattgtatat ttgctacctt
    13981 ggtttataag actgctttga tgcagaattt acttggaaat tcactttcac aatacatacc
    14041 tgtgattatt gcaattttct taacttctgt agtgtatttg atatattaca tacttactat
    14101 taaatcctgt tgtaaaattg tattaaaaaa ataatattta ccaaatcgtc aagcttaaag
    14161 gtgtgacgat ttggtatact tcatttataa gtatttgata taatttaaaa ttataaattc
    14221 ttaagtttga tttagtcata aaattaatat aattaactca taaggaggtg tataagattg
    14281 acaaatttta atttaaaaat actattttta ggaaactaca ttctatcact tctcttcatg
    14341 ggaatatcta caagcactga aacttatatg aatatccaaa gcattattcc cttaataatt
    14401 tcaacattat tactcataga catcacatat ataacctctt gtaatgtccg agaaaataac
    14461 attatttcat tgttttgtgg attactagca ctagacagtt ggtatttact cttatcattt
    14521 gtagccacac caataggcac tattattttt aaaatattaa gcacagttat agcgtatgtc
    14581 tctattaaat ttattttttt atttttgttt caaggtagtg gatacaagtt ccgtaagata
    14641 acagacattt tattgcttat cacctgcatt ggtgcaattg taggagcatt tatttctaac
    14701 agaacgtatg cttgtatgta cggaatacag tttgttgtta gtataataag ctttttctat
    14761 attatttttt atcattggaa aagggtcgct tttgtcataa aaagtgaaaa aaaacctata
    14821 tcgatttcgg tatttataac aattatagga ttccttactt attatttttt tacactaaga
    14881 atatcaaatc atattggtaa ttttggaatc tatctaactg tattagtatt ttttatgagt
    14941 attcatggta ttgcacttaa agaaaatgat agttttccta tttcaacagt ttttaacata
    15001 aagcaaaggt ctatatttct ttttataagt attttagtaa ttagcttggt tgtactattt
    15061 gccagtggga atttttatat aatattaata gctataaatg ttttatttat gcttattttc
    15121 ctctgtaaca ttattttgga gtttaacctt agaaatgaaa aaaataaggt agttaaaaat
    15181 agcaagtata cttttgcatt ggaaaaatta caacaagaag aacaactaaa aacagagttt
    15241 tctaattttt tacatgatga tatattacaa aacctgcttt caataaaaaa catgatgagt
    15301 aagtcatatc gcccagaagt tcaagatatt atatatgaaa cacttaacaa tctaaataca
    15361 tgtataagaa aacaaatgca agactatcac cctgttattt taaaaaattt aacagtaaaa
    15421 gaaaattatc agaatttaat tgaaagtgtt tcttcatcct ttccacaaaa aaacatttgt
    15481 gtttcattta agtgttctga tactttattc ttagttgaac catatgatat attgatatat
    15541 cgactgatta aagaattatt gacaaacgta tataaacatt caaatggtag ccatgcatgg
    15601 atagctttag cactagaaaa aaatataatt aaattaaata ttaatgataa tggtgtgaaa
    15661 aatacatctt ttaaaacgcc tttagatata actaaaatta cttttgatac aataaaacac
    15721 aaagggatta tctcaattaa agaacaagta gaaaatctag gaggaacgat aagtatttct
    15781 gataatatac ctcatggagt ttgtgtacag attacaatcc ctatgaaagg agatggttct
    15841 tataaatatt ttgttagttg atgaccatat gctttttgct aaaagcttgt ccattgcact
    15901 agaagaatac gaagaaatcg aacagtttta ttccacacag gacatccata gtctatcaga
    15961 aatgattcaa gataaaaaga tagacattgt cttaatggat ataaacctta gaggcttaag
    16021 tgaatgtgat ggcttaaaag tagcttataa tttattagag actaattcaa ctattaaaat
    16081 aataatcttg actggatacg acctcccagt ttataaacat gaagctaaaa aaataggtat
    16141 ctatggattc ctaaataaga atattagccc tgataattta attgattcat tattaaaagt
    16201 ctatcaaggg ggaacttgtt ttccatctga aaatactgaa gtaattattg aagatttaac
    16261 tagtatggag aaaagaattt tacagcttat ctgtagtggt aaaaaaagaa aaataattgc
    16321 tgatgaattg tatataagtg aaagaacatt atcaaatcat ttacaacaca tatatgacaa
    16381 gctagatgtt tcatcatcag ttgaggctat tacaaaagcc attcaaatgg gttatatttc
    16441 tccagtatat taaaaaaagt gggtgtctca aaatgagctt tctagctcat gaggcactct
    16501 cctttgtatt aaatcaaata tattttcatc atttaaataa ttaaaaagag gcttctccct
    16561 attttgagac aattccattt ttaatacact agttatactt atataagcat attttaaaca
    16621 actaaaaagc aactaatatc ttaataaata tctcaataca tatcctcata acacatatca
    16681 ccataattac aagcagataa agaactctcc tcacttaaat acgactcaaa ttcataccca
    16741 aacagaattg ttggttctat atatatttcc atatctgtat atctccatct tcttaccttt
    16801 gcctctataa ccttgtaaaa atcttctaaa ctataccctt cttttaatct tttatctata
    16861 agtgccttag ttatacttaa atcagcttta aaattttttc ccgattcact atttaatttt
    16921 cttataataa tttcatgtat actttctgga tttttataag tagaatcttc agtcaaatct
    16981 tcgtccgaat tttcaaaggt atttttattt aaattctttt tatttaaatt attttttaat
    17041 aattcttttt tctttgtctc attttctgtt tcatacccag tatgaattat caaatcataa
    17101 tctttgtcta tactctcttc acttatacga ctgtatatgt tggtattact attcttgaac
    17161 aactcatctc tatgtttata tttcattgtt ttttggttat tgttaatagt tacatcaatg
    17221 tatttctcta tatttttgaa aatatcacca ttctttgcta caatttctgc attactattt
    17281 tcagaacaaa cataactata tacagaacaa ctattcccct ttactcccct acttattaat
    17341 ctcaatatac ctaaatctat aaattcattt acaagtctac ttattgtaga tatagataca
    17401 tcaaggtcag atgcagcttt ccttactgtc atcataaatt gtcctcttgg cagagttcct
    17461 tttaatcttt gtatattttc ttttctcatt acatagtgat taaatcttat tctatttata
    17521 tctctttttt cattaaaata taatactgct gaatttaatt gataaaaact attactcata
    17581 ttcccactcc tccaatttaa atagataatt tgttgtaaag tttttagatt tctaaataac
    17641 ttattaaatg attattttta ttaaaaagta ttttttgata ttttcatttt aaagtt
//
LOCUS       DAEPXF010000076        17478 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain HB27
            SAMN13376389-rid12479423.denovo.080, whole genome shotgun sequence.
ACCESSION   DAEPXF010000076 DAEPXF010000000
VERSION     DAEPXF010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13376389
            Sequence Read Archive: SRR10518432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 17478)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17478)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001069624.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001069624.1
            Genome Coverage       :: 315x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/15/2021 01:54:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,165
            CDSs (total)                      :: 4,076
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 89
            rRNAs                             :: 10, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 10 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 19 of 69
            Pseudo Genes (incomplete)         :: 44 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 10 of 69
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17478
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13376389-rid12479423.denovo.080"
                     /strain="HB27"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="China: Hebei"
                     /collection_date="2017"
     gene            complement(69..947)
                     /locus_tag="KRL67_002155"
     CDS             complement(69..947)
                     /locus_tag="KRL67_002155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HBH1523029.1"
                     /translation="MRYDIAIIGSGPAGLSAAINAKIRNKTIIMFGNDNLSNKLVKAP
                     SIDNYLGFYDISGDELKDKFKSHIDSMDISIENKRINNIYAMGEYFTIMSGNDMYEAT
                     TVILATGVEYTRPIKGEEEFLGRGVGYCATCDAPLYRNKKVAVIGYNEESKEEANFLS
                     ELTSKTYFIPMYKKDNLMRSSDNLDDSIEVIHDRPVQIDGDKLVNKVSFKENHIEVDG
                     VFVIKDSTAPSALVPGIEIDGIHIKVDNNMKTSIDGCFAAGDCVGKPYSYIKAAGQGQ
                     IAALNAVYYLDKLKRA"
     gene            complement(964..1281)
                     /gene="trxA"
                     /locus_tag="KRL67_002156"
     CDS             complement(964..1281)
                     /gene="trxA"
                     /locus_tag="KRL67_002156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HBH1523030.1"
                     /translation="MAKVINTSEFRSSVEGSKGIVVVDFFATWCGPCNMLGPVFAELG
                     EEMKDKARFVKVDIDESLEIAQQFNVSTVPTMIIFKDGKPVETLIGFMPKNKIEMQVK
                     SYL"
     gene            complement(1431..2663)
                     /locus_tag="KRL67_002157"
     CDS             complement(1431..2663)
                     /locus_tag="KRL67_002157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBH1523031.1"
                     /translation="MINKNVFTSTKNHLIKMYIIVVGSFLIIFSIFIYSYFRGLTYSG
                     IDSEINDELEYIVSQFKRTSFLNPIRLKNPKDLVYVYEDGRISYYTQNEYFDELLPDR
                     RLDKKNSFFKYTENGYTFRELNVDVGRYQIQIIRNIDSEMNSLRQLTSVLIIGILISV
                     IITYFVAVYLTRKALIPIETAWKNQAKFIQDASHELRTPITIVSSKLESMLKSPESTV
                     NDEVETIATAMKETRRLKKMITDLLSLTKEDSIVKVNLEEIDLEKLLEEISEDYIDIA
                     EFQEKKFVFNSKLKNKVIITDKNKLRQLILIFIDNAFKYTKSGDEIVLELREDIEDEV
                     ILLISDTGIGIKKEEIPLIFDRFFRSENVRNKDLEGSGIGLSIARMISLNLSIEINVT
                     SDIDIGTTFELSIPKKLK"
     gene            complement(2653..3324)
                     /locus_tag="KRL67_002158"
     CDS             complement(2653..3324)
                     /locus_tag="KRL67_002158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBH1523032.1"
                     /translation="MKVLIVEDNKILLESVVEELSKHFETEKCEDGEEALYLVNQNIY
                     DLVILDLMLPNINGFDILKKMRVNNIDTPVLILTAKETLDDKVEAFTIGANDYLTKPF
                     YMEELVARVYAILRTNGKIKERNGLEFKSLYLDTLEKRVYIEKEEIKLQNKQFNLLEY
                     FLLNKGSILLKEQIYDRIWGIDSDATIEIVEVYVSNLRKKLSKYGYDKYIKTKRKVGY
                     IFDDK"
     gene            complement(3340..4176)
                     /locus_tag="KRL67_002159"
     CDS             complement(3340..4176)
                     /locus_tag="KRL67_002159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH1523033.1"
                     /translation="MNKIVKKSISLMIILTIFIFTLTACEKDEQPDSVDIQAEDKNEI
                     KIDEGNAKVLNIGKSEIINIDEGDKVDTSTKVENNSTFNISNVELIYNEYDENKKIIS
                     SDSKALLDMTLMPGKVAYIECGHKTFVKSVEVYAYEYEAEGKIVYVNLKENTINIRNN
                     NIKLENSSQYEVLSTSELKKVNESNEGITYQVKVKNSSSKDLGNIILKTAEVNENGEY
                     LTVNRVPSYKILKPSEETDIDILCSTKAKSVEIVGYTYDDIKEKANVDIDLKSHKVKI
                     DK"
     gene            complement(4202..4876)
                     /locus_tag="KRL67_002160"
     CDS             complement(4202..4876)
                     /locus_tag="KRL67_002160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HBH1523034.1"
                     /translation="MNNNEDRIDKFLELQSKGFKFAEIAQNLKISQSTLRTFLNKKGY
                     KSEKGKYILKDELNYNKSLDNRNLEEEKAKEYEKSKKNTISNRKKVSTKKKISNEKAL
                     SKSVEQIEFNDAGLKNAKPKQNVKPKKDRKINITQEDLDKLCEVYDWYLEVKDYNIKP
                     KKVSNKKDVHIDDEHIDELKTTRIRIDKEIWNDFERLCSNSNFEKHQILTQALKEFMK
                     KYKNLL"
     gene            complement(4890..5618)
                     /locus_tag="KRL67_002161"
     CDS             complement(4890..5618)
                     /locus_tag="KRL67_002161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HBH1523035.1"
                     /translation="MMFDRGKWNKLDYNELLEYMMSIQDIKYRDFNKKLIPGTENIIG
                     IRVPNLRKLSKEISQGNWKKFLEVAEDTYYEEVRLQGMIIGNINSNFEETLYYVKRFI
                     PKIDNWSVCDGFCSGLKSMKKYKENMYDILKNYVYSKNPWEIRFALVMFLIYYVDDKH
                     IKEIFEYCNNIQSEEYYVKMGMAWLLSICFIKCEEETFLYIKNNNLDDFTYNKTLQKI
                     IESNRVDLEKKNIIRSMKRKTRRC"
     gene            complement(5745..6434)
                     /locus_tag="KRL67_002162"
     CDS             complement(5745..6434)
                     /locus_tag="KRL67_002162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HBH1523036.1"
                     /translation="MDRYSEITNKNQREIVLLKGFPCIWGKCSFCDYIDDNSNLEEEM
                     NKLNLKVLKNVTGKYGVLEVINSGSCFELPKDTLEKIKCIIKEKNIKKLFLESHWSYK
                     NRLKEMREYFEIPVVFKIGVETFDNDFRNNILNKNANFKTPQDVKEYFDSPCIMVGIK
                     GQTKEMIDKDIEIILNTFEKATVNVFINNSSSIKRDEELVKWFVSKYKFLDENPNIEV
                     LYNNTDFGVGD"
     gene            complement(6442..7005)
                     /locus_tag="KRL67_002163"
     CDS             complement(6442..7005)
                     /locus_tag="KRL67_002163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HBH1523037.1"
                     /translation="MKRKICVKTITLIAFGISINIIGAFIAMGLRLPVYLDSIGTIMI
                     ASLLGPKYAVVTGVFGSLISGITFDVYSLYFAPVQISTGLLAGIAFKKNFLRGLKTPL
                     GVLLFAIPTSIISSIISAFLFGGMTSSGSSYIVQILKVLGLGDVFSVFITQVFTDYGD
                     KLLAVVLVNLGLNAVPKTLKISLTGGK"
     gene            complement(7007..7975)
                     /locus_tag="KRL67_002164"
     CDS             complement(7007..7975)
                     /locus_tag="KRL67_002164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HBH1523038.1"
                     /translation="MEKRKVIIDCDPGIDDSLAILLALNSPELEVIGITTCCGNVPAN
                     IGAENALKTLQMCSSLNIPVYIGEEAPLKRKLVTAQDTHGEDGIGENFYQKVVGAKAK
                     NGAVDFIINTLHNHEKVSIIALAPLTNIAKALIKDKKAFANLDEFVSMGGAFRIHGNC
                     SPVAEFNYWVDPHGADYVYKNLSKKIHMVGLDVTRKIVLTPNIIEFINRLDKKMAKYI
                     TEITRFYIDFHWEQERIIGCVINDPLAVAYFIDRSICKGFESYVEVVEDGIAMGQSIV
                     DSFNFYKKNPNAIVLNEVDEKKFMYMFLKRLFKGYEDIIDSVEGVI"
     regulatory      complement(8076..8119)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            8484..9113
                     /locus_tag="KRL67_002165"
     CDS             8484..9113
                     /locus_tag="KRL67_002165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBH1523039.1"
                     /translation="MSIKERRKNEKEEMKKKIMDASIEIINQHGYENLSIRKIATKIE
                     YSPTTIYLYYKDKAEIISDMTNKLYNTVESNAIDIMNNCSTLPIDKQIIEIMTSFIKT
                     LSSEPEMSKAIMHSRMNIIFASENINNTPSNNGIKMLDKFLSIGIEQGIFKKNIDKSS
                     WMLISALLGFVLCVVENKLYSLHNFSQLIENFLDILVGGLYNENSQQSY"
     gene            9088..9492
                     /locus_tag="KRL67_002166"
     CDS             9088..9492
                     /locus_tag="KRL67_002166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein"
                     /protein_id="HBH1523040.1"
                     /translation="MKIHNKATNKKKKYVKISIALFFVFICVIIVIVSNLKPENLVVK
                     DIDINNVKNGIYTGSADNKLVKATVSVEVNNGKIQNINILKHDHLLGKPAEKITTSII
                     KQQSLDVDAITSATYSSNTIRKAVENALRKGE"
     gene            9495..9971
                     /locus_tag="KRL67_002167"
     CDS             9495..9971
                     /locus_tag="KRL67_002167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin domain-containing protein"
                     /protein_id="HBH1523041.1"
                     /translation="MNTIIIYSSKYGCTKDCANILKNKLSDNVTIVDINNNNNKIELS
                     KFDKIIIGSSIYVGSVSKKIQVLCNDNVELLNKKQVGIFLCCGFSEQADKYLKSNFPS
                     SLLESANAIGIFGSEARLEKMKFLDKLIMKAVSKGNYDSFRISQDNIDNFLINLNS"
     gene            10519..11184
                     /locus_tag="KRL67_002168"
     CDS             10519..11184
                     /locus_tag="KRL67_002168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF969 domain-containing protein"
                     /protein_id="HBH1523042.1"
                     /translation="MIKLIGILIVVIGFILKIDTLFTVLLAGVATGIVAGLDFNQIFT
                     ILGDSFVSNRGVSLFILTLPVIGVLERYGLKQRAVSLIEKLKRLTTGKVLTIYMIARQ
                     IAGALSIRMSGHPQFVRPLVNPMAQAAGLSNSDELKESDEEAIKALSAASENYGNFYG
                     QNLFAGSSGVLLIASTLTQFGYNVTGLNIVKANIIMAVIALVVATIQFTLYDKKLNKS
                     HKK"
     gene            11206..12138
                     /locus_tag="KRL67_002169"
     CDS             11206..12138
                     /locus_tag="KRL67_002169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF979 domain-containing protein"
                     /protein_id="HBH1523043.1"
                     /translation="MQNFINISLEIFYALMGFLMIVIAYKSFTTINNNKKYGTSLFWI
                     LISLPFIFGRLIPANIIGIILILSSLLTLSKQVVFAKYEEPDENFGKEQADKLKNKIF
                     IPSLILAFAAVVVAMSLSNFDNSSQFAIGVGSIIALISALIITKAKPATSVQDGSRLL
                     QQMGPASMLPQLLVALGALFTQAGVGEVISTMISGVVPADSRLFGVIAYVLGMVIFTM
                     IMGNAFAAFSVITAGIGIPFVLSQGGNPAIIGALALTAGYCGTLLTPMAANFNIVPAA
                     LLECKNDYIVIKYQAPVALVLIIAHILVMYFLGF"
     gene            12159..12800
                     /gene="pcp"
                     /locus_tag="KRL67_002170"
     CDS             12159..12800
                     /gene="pcp"
                     /locus_tag="KRL67_002170"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HBH1523044.1"
                     /translation="MKILLTGFDPFGGEPINPAQEAVERVNNNINGAEIIKITIPTVM
                     TKSVEAIDKAIQEHNPDIVISVGQAGGRFDITPERVAINIDDFRIKDNEGNQVIDTII
                     KEDGEPAYFSKLPVKAMVKHMNENKIPASVSNTAGTFVCNHVMYGILYMIDKKYPNIR
                     GGFIHIPYTTSQVIDKKNTPFMSLEEIVKGLELAIEACIIYKEDVKEIGGEIS"
     gene            complement(12920..13252)
                     /locus_tag="KRL67_002171"
     CDS             complement(12920..13252)
                     /locus_tag="KRL67_002171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBH1523045.1"
                     /translation="MSIDCISNTDLSETGFSYTLSLISGKYKMIILYCLVEFEVVRYN
                     ALKRYINTISYKTLSLSLKELEADNLIIRTEYPQIPPKVEYSLSERGKSLIPILDAMC
                     EWGERNRP"
     gene            13518..14138
                     /locus_tag="KRL67_002172"
     CDS             13518..14138
                     /locus_tag="KRL67_002172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HBH1523046.1"
                     /translation="MKVLLINGSPNQYGCTYTALNEITKVLSKHDIKTEILYLGKEAI
                     PGCISCASCFETGKCIRNDKVNELIEDLDNIDGIIIGSPVYFSSATGQLTSFLDRLFF
                     IAGSRMATKLGASVVSCRRGGASATFDQLNKYFSISNMPIVSSQYWNQVHGFTPEDVM
                     KDEEGLQTMRTLGENMAWLLKCISAGKKAGIKEPQYEETIMTNFIK"
     gene            complement(14463..15287)
                     /locus_tag="KRL67_002173"
     CDS             complement(14463..15287)
                     /locus_tag="KRL67_002173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraX"
                     /protein_id="HBH1523047.1"
                     /translation="MDFFKKGISGFTIKILALIFMTFDHIAAFMPQTMQIPIWFHWVG
                     RISAPLFIFMAVEGFYHTSNRKKYISRLYVWSVIMAIGNQIINNVFSHPEGAIIINNI
                     FSTLFLIAVYLQAIEFIKKFRKEKEIKYFIIGLLMIIIPIILGIFTVALLFKVTNRVI
                     ALFMILVPVPFLVEGGPVWIILGIVFYLCRGKKFRLSLYYVLMCIFIFVAMANGDYSL
                     KNSILQNYQWMMIASLPLMLLYNGEKGKSMKYLFYLYYPIHLYILYILGIYLINGF"
     gene            complement(15298..16701)
                     /locus_tag="KRL67_002174"
     CDS             complement(15298..16701)
                     /locus_tag="KRL67_002174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBH1523048.1"
                     /translation="MKWKITRNFIFTIVFVVISVVIINIISILYVVSTDSFFKVVDSG
                     NNPEEFARSFEKDLYEKDGEFKLSKIGAEKLEKSNSWIQVLNDLGEEVYGVNVPKDTP
                     KKYTPFQMVNNYKYIETKYVNFVLEKYLNKKHLNIIVGIPSRDISRIILTYSQNNIKK
                     TLNKVIIITLVIDSVVALGVGYLFSRKLTKPISSVLWSIETMANGNYSLYLKDRGIYE
                     EVFKNINMLADTLRVNEVERKENEELREEWLANITHDIKTPLASIQGYAEIINDKNYE
                     FEEDEIREYTEIIYNKSKYIKDLVDDLNLSTRLKNNTIVLDKKKINLVSLVRDIIIDI
                     LNDNRYKNRNIEFESNEDLIEVHVDSILFRRAITNLIFNSIVHNSEGTLISVEIVKKD
                     NIEIIIKDSGIGISKSDLKHIFKKYYRGTNTGEMHKGSGLGMAISKEIIEIHKGKIYV
                     SSEIGIGTKIIIEIKQN"
     gene            complement(16705..17430)
                     /locus_tag="KRL67_002175"
     CDS             complement(16705..17430)
                     /locus_tag="KRL67_002175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012450879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBH1523049.1"
                     /translation="MENRVLIIDDEVEILKLLETVLKKEGLSNIYTAKTKEEGLEVFK
                     SINPDLIVLDIMLPDGEGYDICKEIRKTSNSPIIFLSAKTEELDKLLGLAIGGDDYVT
                     KPFSPKEVAFRVKAHLRRLSYFSDIQNESKNLNNNEEKIISFGPYILNESRAELIKDG
                     KSIGLSAKELKILSLFAHNQNQIISKEKLWDKVWGEDYVGFDNTIMVHIRKIREKLED
                     NPSKPEYILTIKGLGYKLAVKED"
BASE COUNT         6018 a         2761 c         1931 g         6768 t
ORIGIN      
        1 ttgaaataat aaaattattt caagacagtt ttttataaat atctttaaaa agttaatcaa
       61 tataaatatt aagccctctt taatttatct aaatagtaaa cagcatttaa agctgctatt
      121 tgcccttgac cagctgcttt tatataagaa tagggttttc caacacaatc tccagcagca
      181 aagcatccat ctatactagt cttcatatta ttgtcaactt taatatgaat gccatcaatc
      241 tctattccag gaaccaatgc acttggagcc gtactatctt ttataacgaa tactccatca
      301 acttcaatgt ggttttcttt aaaagatact ttgtttacaa gtttatctcc atctatctgt
      361 actggtctgt catgtataac ttcaatagaa tcatctaggt tatctgaact tctcataaga
      421 ttatcttttt tgtacatagg tataaaataa gtctttgaag tgagttcact taaaaaattt
      481 gcttcttctt tagattcttc attatatcct ataactgcta cctttttatt tctataaagt
      541 ggtgcatcac atgtagcaca atagcctaca cctcttccta gaaattcttc ttcacccttg
      601 attggtctag tatactctac cccagttgca aggattacag tagtagcttc atacatatca
      661 tttccagaca ttattgtaaa gtattctccc atagcataaa tattatttat tcttttattt
      721 tcaattgaaa tatccatact atctatatgt gatttaaact tgtcttttaa ttcatcacca
      781 cttatatcat aaaagcctaa ataattatct attgaagggg ctttaacaag cttattactt
      841 aaattatcat taccaaacat gataattgtt ttatttctta tttttgcatt tatagcagca
      901 gacaatccag ctggaccact gccgattata gctatatcat atctcataat actcacccat
      961 tttttataaa tatgatttta cttgcatttc aattttgttc tttggcatga atccaattaa
     1021 agtttctact ggttttccat ctttaaatat aatcatagtt ggtactgtag atacattgaa
     1081 ttgttgagct atttctaagc tctcatctat atcaaccttt acaaatctag ctttatcttt
     1141 catctcttca cctaattctg caaatacagg tcccaacatg ttacaaggtc cacaccaagt
     1201 tgcaaagaaa tcaactacaa ctattccctt actaccttca acactacttc taaattcaga
     1261 tgtatttata acttttgcca tttttaaatc ctcctattaa actttattat ccctatataa
     1321 ttatactatg aaatttataa atataaattt acttttataa ggtcttttta tattttatac
     1381 taattagtat acccattctt catttctaaa aacaattttt tatattttta ttattttaac
     1441 tttttcggta ttgacaactc aaatgtagtt ccaatatcta tatcagaagt tacatttatc
     1501 tcaatagata agtttaagct aatcatacga gctattgaaa gtcctattcc agaaccttct
     1561 aaatctttat ttcttacatt ttcgcttcta aaaaatctat caaatataag aggtatttct
     1621 tcttttttta tgcctatacc agtatcactt ataagtaaaa ttacctcatc ttctatatct
     1681 tctcttaatt ctaatactat ctcatcaccc gattttgtat atttaaaagc attatcaatg
     1741 aaaatcaata taagctgtct taatttattt ttatctgtta ttataacttt attcttcagt
     1801 ttagaattaa acacgaattt tttttcctga aactcagcaa tgtcaatata gtcttctgat
     1861 atttcttcca ataacttttc taagtctatt tcttctaaat taactttaac aatagagtcc
     1921 tctttagtaa gagacaataa atcagttatc atttttttaa gtcttctagt ttccttcatt
     1981 gcagtagcta ttgtttcaac ttcatcattt actgtacttt ctggtgattt taacatactt
     2041 tctaattttg atgaaactat tgttattgga gttcttaatt catgagaagc atcttgtata
     2101 aacttagctt gatttttcca tgctgtttct attggaatta aagcttttct agtaagatac
     2161 acagccacaa agtaagtaat tattacagaa attaatattc caattataag aacagatgta
     2221 agctgtctta aagaattcat ctcggaatct atatttctaa ttatttgtat ttggtatcta
     2281 cctacatcta catttaattc tctaaaagta tatccatttt ctgtgtattt aaaaaacgaa
     2341 ttttttttat ctagtcttct atctggaagc aactcatcaa aatattcatt ttgagtatag
     2401 taacttatcc ttccatcttc atatacatac accaaatctt tagggttttt gagtcttatt
     2461 gggttcaaaa atgaagttct tttaaattga gaaactatat attcaagttc atcatttatc
     2521 tcactatcaa ttccactata tgtaagtccc ctaaaatatg agtatataaa tatagagaag
     2581 attattaaaa aactacctac tacaataata tacattttta ttaaatgatt tttagtactg
     2641 gtaaatacat tcttatttat catcgaatat atatcctacc ttacgtttag ttttaatata
     2701 tttatcatat ccatatttac ttagcttttt tcttagatta cttacatata cttccactat
     2761 ttcaatagtt gcatctgaat caattcccca tattctatca taaatctgct cttttaataa
     2821 aattgaacct ttatttaata gaaaatactc aagcaagtta aactgtttgt tttgaagttt
     2881 tatttcttct ttttcaatat acactctttt ttctagtgta tctaaatata aagatttaaa
     2941 ttctaatccg tttctttctt ttatctttcc attagtcctt aatatagcgt atacccttgc
     3001 tactaactct tccatataaa aaggttttgt caaataatca ttagctccta tagtaaatgc
     3061 ttctactttg tcatctaatg tttcttttgc tgtcaatata agtacaggtg tatctatatt
     3121 attaactctc attttcttta ggatatcaaa tccatttata ttaggaagca ttaagtctaa
     3181 aataactaaa tcatatatat tttgatttac taagtataaa gcttcttctc catcttcaca
     3241 cttttcagtt tcaaaatgct tgctaagttc ttctactaca ctttctaaaa gtatcttatt
     3301 atcttcaact attaaaacct tcataagtat cccctacctt tatttgtcta tcttaacttt
     3361 atgagatttt aagtcaatat ccacattggc tttttctttt atatcatcat acgtatatcc
     3421 cactatttca acactttttg cctttgttga gcacaatata tctatatctg tctcttctga
     3481 aggctttaaa atcttatatg atggcacacg atttactgtt aaatattctc cattttcatt
     3541 aacttctgct gtcttaagaa taatatttcc taaatctttt gatgatgaat tttttacttt
     3601 tacttgatac gtaattccct catttgattc atttactttt ttaagttctg atgtagatag
     3661 cacctcatac tgactactat tttctagctt aatattatta tttcttatat taatagtatt
     3721 ttcttttaaa ttaacataca caatcttacc ctctgcctca tattcatatg cataaacttc
     3781 tacactcttt acaaaagttt tatgcccaca ttctatgtaa gcaactttcc caggcattaa
     3841 agtcatgtcc aacaaagcct tactatctga agatataatt tttttattct catcatattc
     3901 attatatatc aattcaacat tggatatatt aaatgttgaa ttattttcta cctttgttga
     3961 tgtatctact ttatcacctt catctatatt tataatttct gattttccta tattcaatac
     4021 ttttgcattt ccctcatcta tttttatctc atttttatct tctgcttgaa tatcaactga
     4081 atcaggctgt tcatcttttt cacatgctgt caacgtaaaa ataaatattg ttaatataat
     4141 cattagagaa attgattttt ttactatttt attcatatta agcacctcat atagtagtat
     4201 tttatagtag atttttatac tttttcataa attctttcaa tgcctgtgta agtatttgat
     4261 gtttttcaaa attcgaatta ctacataatc tttcaaaatc attccatatt tctttatcta
     4321 tacgtattct tgttgttttt aattcatcta tgtgttcatc atctatatgt acatcttttt
     4381 tattagatac ttttttaggc tttatattgt aatcttttac ttctaaatac caatcatata
     4441 cttcacaaag cttatctaaa tcttcttgag ttatatttat ttttctatct ttcttaggct
     4501 taacattttg ttttggcttt gcatttttta aacctgcatc attaaactca atttgttcaa
     4561 cacttttact taaagccttt tcattagaaa tctttttttt ggtgcttact ttttttctat
     4621 tacttatagt attcttttta cttttttcat actctttagc tttttcttct tccaagtttc
     4681 tattatctaa acttttatta taatttaatt cgtcttttaa tatgtattta cctttttctg
     4741 acttatatcc ttttttattt aaaaaagttc ttaaagtact ttgactaatc tttagatttt
     4801 gagctatctc tgcaaattta aatcctttgc tttgaagttc taaaaatttg tcaattctgt
     4861 cttcattgtt attcattaat attcctccgt caacatcttc ttgtctttct tttcatactt
     4921 cttattatat tcttcttttc taaatctact ctatttgatt caattatttt ttgtagagtt
     4981 ttattataag tgaaatcatc taaattatta ttttttatat agagaaaagt ttcctcttca
     5041 catttaataa aacaaattga tagcaaccat gccataccca ttttaacata atattcttca
     5101 ctttgtatat tattacagta ttcaaatatt tctttaatat gtttatcatc aacataatat
     5161 attaaaaaca taactaatgc aaatctaatt tcccatggat tctttgaata aacataattt
     5221 ttaagtatat catacatatt ttctttatat ttcttcatag actttaaacc agaacaaaat
     5281 ccatcacaaa ctgaccaatt atcgatttta ggtataaatc tttttacata gtataaagtt
     5341 tcttcaaaat tactatttat attgccaatt atcatacctt gtagcctaac ttcttcatag
     5401 tatgtatcct ctgctacttc taaaaatttc ttccaattcc cttgacttat ttcttttgat
     5461 aactttctta aattaggaac tctaatacct attatatttt ctgttccagg tataagcttt
     5521 ttattaaaat ctctatattt tatatcttgt atagacatca tatattctaa aagttcatta
     5581 taatccaatt tattccattt tcccctatca aacatcattt aattactcct taattccttt
     5641 tttcttattg taacataaaa ctctacaaaa aaagcttcta actattagaa gctttttcaa
     5701 aaacataaga aacttttcaa ttttaaattt aaactaaatt aacactaatc tcctacacca
     5761 aaatctgtat tattgtataa aacttctata tttggatttt catccaagaa tttatactta
     5821 ctaacaaacc atttcactaa ctcttcatct ctttttatac tagaagaatt atttataaaa
     5881 acattcactg tcgctttttc aaaagtattt aagataatct ctatatcttt atctatcatc
     5941 tcttttgttt gtccttttat tccaaccata atacaaggtg aatcaaaata ttcttttaca
     6001 tcttgtggag tcttaaaatt tgcattttta tttaaaatat tatttctaaa atcattgtca
     6061 aaggtttcaa ctccaatttt aaataccaca ggaatttcaa aatactctct catttcctta
     6121 agcctatttt tgtaagacca atgactttct aaaaataatt tttttatatt cttctctttt
     6181 ataatacatt ttattttttc taaagtatcc ttaggtaatt caaaacaact tccagaattt
     6241 atgacttcaa gaactccata tttcccagtt acgtttttta aaacctttag atttaattta
     6301 ttcatctctt cttctaaatt agaattatca tctatataat cacaaaaaga acattttccc
     6361 catatacaag gaaatccttt aagcagaaca atttctcttt gatttttatt tgttatctca
     6421 ctatatctat ccattttcta tctattttcc tccagttaat gatatcttta aagtttttgg
     6481 aactgcattt aatcctaaat tcactaaaac aactgctaac agcttatctc cataatcagt
     6541 aaatacttgg gttatgaata cactaaaaac atctcccagt cctaacactt tcaagatttg
     6601 cactatatag gatgagcctg aagaggtcat acctccaaat aaaaatgctg aaattattga
     6661 acttattata gatgttggaa tagcaaataa aagtactcct agtggagttt ttaaacctct
     6721 taaaaaattc tttttaaatg ctattccagc aagtaatccg gttgatattt gaactggtgc
     6781 aaaatagaga gaatacacat caaacgtaat tccacttatt aaacttccaa acacacctgt
     6841 aacaacagcg tatttaggtc ctaaaagtga agctatcatt atagttccta ttgaatctaa
     6901 atatacaggt aatcttaatc ccatagctat aaaagcgcct attatattta tggaaatacc
     6961 aaaagctata agtgtaatag tttttacaca tatttttctt ttcacactat atcactcctt
     7021 ccacagagtc tataatgtct tcataacctt taaaaagcct ctttaaaaac atgtacataa
     7081 atttcttctc atcaacttca tttagaacaa ttgcattagg atttttttta tagaaattga
     7141 aagaatccac tatagactga cccatagcta ttccatcttc tacaacttct acgtatgatt
     7201 caaatccttt acatatactt ctatctataa agtatgctac tgctagaggg tcatttatca
     7261 cacagccaat tattctctcc tgttcccaat ggaaatcaat ataaaatcta gttatttcag
     7321 ttatatactt tgccatcttc ttatcaagtc tatttataaa ctcaataata ttaggagtaa
     7381 gtacaatttt tctagttaca tctaaaccta ccatgtgaat ttttttagat aaattcttgt
     7441 aaacataatc tgctccatgt gggtctaccc aataattaaa ctctgctact ggagagcaat
     7501 ttccatgaat cctaaatgct cctcccatag atacaaactc atcgagattt gcaaatgctt
     7561 tcttatcttt aataagtgct ttagctatgt ttgtaagtgg tgcaagtgct attattgata
     7621 ctttttcatg attatgtaaa gtatttatta taaaatccac tgctccattt tttgctttag
     7681 ctcctacaac cttttgataa aagttttctc caataccatc ttctccgtgt gtatcttggg
     7741 ctgttacaag ttttcttttt agtggtgctt cttctcctat atatactgga atatttagtg
     7801 aagaacacat ttgaagtgtt tttagtgcat tttctgcacc tatatttgct ggaacatttc
     7861 cacagcatgt agtaattcca attacttcta gctctggtga gtttaaagcc agaagaattg
     7921 ccaaagaatc atcaattcct gggtcacaat caattattac ttttctcttt tccatttttt
     7981 atcctccttg atttgtggtc gaggagaact acacatcttt tttccacttt caaaaaggtg
     8041 tgtctaaggg tattaattat atactgccac tatataattc cttagttttt tatacttgga
     8101 gttctcgaac aagtacagat gtaaaatcat acttttaggc tacttactat agattcttaa
     8161 aaactataga ctgttaaaaa atttttaaac atctgattta atagctataa aataaatttt
     8221 atagcataaa agaatttatt atattttaat gtatcttatt aaagtttatt tagaattatg
     8281 aaccaacttt aataagatgt ggcgcattta aaccgattat tattatacaa taatatctta
     8341 aatattacaa ttaataacat taatttatta gccataaaaa tatattaact tatattattt
     8401 aataatttat tgacagtatt aaaattaaat tatacaataa cagtgtaaat taacttaaca
     8461 ccgttaagca aagaggtgat tatatgagta tcaaagaaag acgtaaaaat gaaaaagaag
     8521 aaatgaagaa aaagattatg gatgctagca tcgaaattat aaaccaacat ggatatgaaa
     8581 atttatcaat aagaaaaatt gctacaaaaa tagaatattc tccaactaca atttatcttt
     8641 actacaaaga caaagcagag attatcagcg atatgaccaa taaactttat aacacagtag
     8701 aaagtaatgc tattgatatt atgaataatt gctcaacgct accaatagat aaacaaatca
     8761 tagaaattat gactagcttt ataaaaactc tttctagtga accagagatg tcaaaagcta
     8821 tcatgcatag tagaatgaat attatatttg catctgaaaa tataaacaat acgcctagca
     8881 ataatggtat aaaaatgctt gataaattcc tttccattgg aattgaacaa ggaatattca
     8941 aaaaaaatat agataaaagc tcatggatgc ttataagtgc actgttagga ttcgttttat
     9001 gtgttgttga aaataaacta tattctttac ataatttttc tcaacttata gaaaatttct
     9061 tagatatttt agtaggagga ctttacaatg aaaattcaca acaaagctac taataaaaag
     9121 aaaaaatatg taaaaatatc tattgctcta ttctttgtat ttatatgcgt tattattgta
     9181 attgtaagca acttaaagcc agaaaactta gtagtaaaag atatagatat taataatgtt
     9241 aaaaatggca tttatactgg aagtgctgat aataaattgg taaaggctac tgtgtctgtt
     9301 gaagttaata atggtaaaat ccaaaatatt aatatcttaa aacacgacca tcttttaggt
     9361 aaacctgctg aaaagattac aacaagtatt attaaacaac agtctttaga tgtggatgct
     9421 ataacttctg caacttatag cagtaatact atacgaaaag ctgtagaaaa tgctttaaga
     9481 aagggagaat aattatgaat actattatta tatattcaag taaatatggt tgtacaaaag
     9541 actgtgcaaa tatactaaaa aataaactat cagataatgt cactattgtt gatattaata
     9601 ataataataa taaaattgag ttatcaaaat ttgataaaat tattattgga agctctatat
     9661 atgttggttc agtttcaaaa aaaatacaag tgctatgtaa tgataatgta gagttgttaa
     9721 acaaaaaaca agttggtatt ttcctttgct gtggcttttc tgaacaagca gataaatatt
     9781 taaaatcaaa ttttccatca tccttattag aaagtgcaaa tgcgattgga atttttggta
     9841 gtgaagctcg attagaaaaa atgaaatttt tagataaact tattatgaag gctgtttcaa
     9901 aaggtaatta tgacagtttt agaatatcac aggataatat agataatttt ttaataaatc
     9961 ttaatagcta aaataaaact aattatctct atgataaatt cttttattta taagtaatat
    10021 tgctcaaaat agtctttaaa ctttattaca attttatttt tcttagtgaa atctaatgaa
    10081 actagaactt aatatatgtt ttattaatca taacaaaaag agagtgtcta atgaactttt
    10141 tagttcatta gacactctct ttttgtatga aatcaaatat atttttatca tttcaacagt
    10201 gaaaaataca cttttctcta ttttgacaca ttctcttttt ataatataat atctaattta
    10261 aaaatactat ttcaaacatt gacatatact taaactatat acttacaaaa tcaattcata
    10321 ttagcttaaa atataatctt tcttattcaa atttctatac acaaccatta tttactttga
    10381 tactttaaaa catcttccct attttttctt aaattttata ataatagtcc tattaacgca
    10441 aatactataa aaagagttta actttatcct ttaaataaat ttaaattaaa acatatagaa
    10501 atggaaggag aacaatttat gataaaacta atcggtatat tgatagttgt aattggattt
    10561 atacttaaaa ttgacacatt atttactgta ttattagcag gagtggcaac aggaattgtt
    10621 gcaggattag attttaatca aatttttaca attttaggag attcttttgt ctcaaacaga
    10681 ggtgtatcat tattcatatt aacattgcca gtaatcggtg tattagaaag atatggttta
    10741 aaacaaagag ctgtttcttt aatagaaaaa ttaaaaagac ttacaacagg aaaagtactt
    10801 actatataca tgatagctag acaaatagct ggtgctctat caataagaat gagtggacat
    10861 cctcaatttg taagaccact tgttaatcct atggcacaag ctgctggttt aagtaatagt
    10921 gatgaactaa aagaatcgga tgaagaagcc ataaaagcat tgtctgctgc atcagaaaac
    10981 tatggaaact tttatggtca aaacctattt gcaggtagta gtggagtatt acttatagca
    11041 tctactctga ctcaatttgg atacaatgta actggactta atattgtaaa ggctaatatt
    11101 attatggcag taatagcact tgtagtagct acaatccagt ttaccttata tgataaaaaa
    11161 ttaaacaaaa gtcataaaaa atagttttat ttaggaggaa taaaaatgca aaattttata
    11221 aacatatcat tggaaatctt ttacgcacta atgggatttt taatgattgt tatagcatat
    11281 aagtctttta ctaccataaa taacaataag aaatatggta catcattatt ttggatactt
    11341 atatcactgc cattcatatt tggtagattg attccagcca atattatagg aataatatta
    11401 atattatcta gtttattaac actttctaaa caagttgtat ttgcaaagta tgaagaacca
    11461 gatgaaaatt ttggaaaaga acaagcagat aaacttaaaa ataaaatatt tataccatcg
    11521 ttaatattag catttgctgc ggtagtagta gctatgagcc tttctaattt tgataattct
    11581 tctcaatttg cgataggtgt gggttcaata attgcattaa tatcagccct tataataacc
    11641 aaggcaaaac ctgctacatc agttcaagat ggctcaagat tacttcaaca aatgggacct
    11701 gctagtatgt taccacaact tttagttgca ttaggagctt tatttactca ggctggggtc
    11761 ggtgaagtta tttcaaccat gatatctggt gtagtccctg ctgatagcag attatttggt
    11821 gttatagctt atgtattagg tatggttata tttaccatga taatgggaaa tgcatttgcc
    11881 gcattttcag tcataacggc aggaattggt attccttttg tattgtctca aggtggaaat
    11941 cctgctataa ttggagcatt agcattaaca gcaggatatt gcggaacttt gcttactcca
    12001 atggctgcta attttaatat agttccagca gccttgctag aatgtaaaaa tgattatatt
    12061 gttataaaat atcaagctcc agttgctttg gtattaatta tagcccacat attagtaatg
    12121 tactttttag gattttaata taattaggag gtaaaataat gaaaattttg ttaacaggat
    12181 ttgacccatt tggtggagaa ccaataaacc cagctcaaga agcagttgaa agggtcaata
    12241 ataatataaa cggtgcagaa atcataaaga taactatacc aacagttatg acaaaatctg
    12301 ttgaagctat agataaagct attcaagagc acaatccaga tatagtaata tcagttggac
    12361 aagctggagg tagatttgac attacacctg agagagttgc tataaatata gatgatttta
    12421 gaataaaaga taatgaaggt aaccaggtta tagatactat tataaaagaa gatggtgaac
    12481 ctgcatattt ttcaaaattg cctgtaaaag ctatggtaaa acatatgaat gaaaataaaa
    12541 ttccagctag tgtttcaaat actgcaggaa catttgtttg taatcatgtg atgtatggaa
    12601 tcctttatat gatagacaag aaatatccta atataagggg tggatttata cacatcccat
    12661 atacaacttc acaggtaata gataaaaaaa atacaccatt catgtcttta gaagaaatag
    12721 taaaaggttt agaattagct atagaagctt gtattatata caaagaagat gtaaaagaaa
    12781 ttggtggtga aatttcataa taaatttttt agtattttta taaattaaaa atactaacta
    12841 ttaatttaaa tatagactgt gagtagatta ttcaaacaat tttactcaca gtctttttat
    12901 ggtatttaca gatataaaat taaggtctat tcctctctcc ccactcacac attgcatcta
    12961 aaattggaat cagagatttt cccctttctg ataaactata ttctacctta ggaggtattt
    13021 gaggatattc agtacgaata atcaagttat ctgcttctaa ttcttttaat gataaactca
    13081 aagttttata ggagatagta tttatataac gttttaatgc attatatctt acaacttcaa
    13141 attctacaag acagtaaaga attatcattt tatatttacc actaatcagt gaaagtgtat
    13201 aactaaatcc tgtttcactt aaatctgtat ttgatataca gtctatactc atatattaca
    13261 ctctcctttt ggttagtatt gaacaaaaat gtgcgtactt gtttttagaa taatttataa
    13321 taaaattata gtgtaagaat aattgaaagt caattttata ataggaggtg tatttactaa
    13381 aaaaagagtt attttaaata ggattctaag attagacatt tctatataaa ttgataatat
    13441 tcatgaaaat cctattttat aaaaaataat atctcttaga aaatctatta aataaaattc
    13501 actggaggaa atttataatg aaagttttat tgataaacgg tagcccaaac caatatggat
    13561 gtacttatac agcactaaat gagattacta aagtattgag taaacatgat attaaaacag
    13621 aaatactgta tttaggtaaa gaagcaatcc caggatgtat tagttgcgca tcctgttttg
    13681 aaactggaaa atgtattcga aatgataaag ttaatgaatt gattgaagat ctggataata
    13741 ttgatggtat tattattggt tctcctgttt acttttctag tgcaactggt caattaactt
    13801 cttttcttga ccgtctattt ttcatagctg gaagtcgcat ggctacaaaa ttaggtgctt
    13861 cagttgtgtc atgtagacgt ggtggtgcaa gtgcaacatt tgaccaacta aataaatatt
    13921 tttcaatcag caatatgcct attgtatcat cacagtattg gaaccaggta catggcttta
    13981 ctccagagga tgttatgaag gacgaagaag gattacagac tatgcgaact ctaggagaaa
    14041 acatggcatg gcttcttaag tgtatatcag caggtaaaaa agctggaatt aaagagccac
    14101 aatatgaaga aacaattatg actaatttta taaaataaat aaaaaatgca ctaatattaa
    14161 ttaaaacaaa acactaattt ataaataaaa taagtataaa tgtaggataa aaagcactaa
    14221 aacagaggta ttgaatttta tatcaaacct ctgttttatt attacattaa taaccaaacc
    14281 atatataaca tcatatattt ctaaatagat tataatataa tattctaatg ttattgaaat
    14341 tacaataact gaatttctgc tcataatttt aaatattcta caaatctcat ctaaataaca
    14401 attttaattg acctaaaaca atcctcatat tgttcatttt gtattaaaaa tatcacttac
    14461 aatcaaaaac catttattaa gtatattcct aagatataca atatatataa atgtattgga
    14521 tagtataaat aaaataaata cttcatactc ttcccttttt ctccattgta aagtaacatt
    14581 aaaggtagag aagcaatcat catccattgg taattttgta aaatagaatt ttttaaacta
    14641 tagtctccat ttgccattgc tacaaatata aatatacaca ttaaaacata ataaaggctt
    14701 aatctaaact ttttacctct acacaaataa aaaacaatcc caagtataat ccaaactggt
    14761 ccaccttcta ctaaaaatgg tactggtacc aaaatcataa ataaagcaat aactctgtta
    14821 gtgactttaa aaagtaaagc tactgtaaat attcctaaaa ttataggaat gattatcatc
    14881 aataatccta taataaaata tttaatttct ttttcttttc taaatttctt tataaactct
    14941 attgcttgta aataaaccgc tattaaaaat aatgttgaaa aaatgttatt tataattata
    15001 gctccttctg gatgtgaaaa tacattatta attatttgat ttcctatagc cataataaca
    15061 gaccaaacat acaatctact tatatatttt tttctgttac tggtgtgata aaatccttca
    15121 acagccataa atataaatag tggtgctgat attctgccta cccaatgaaa ccaaattggt
    15181 atttgcattg tttgtggcat aaatgctgct atatggtcaa atgtcataaa tattaatgct
    15241 aaaatcttaa tagtaaaccc acttatacct tttttaaaaa aatccatatc atatctccta
    15301 attttgtttt atctctataa taatttttgt tcctattcca atttcactac ttacataaat
    15361 tttaccttta tgaatctcta ttatttcttt agaaatagcc ataccaagac ctgaaccttt
    15421 atgcatctcc cctgtattag tccctctata atatttttta aaaatatgtt tcaaatcact
    15481 tttgcttatt ccaattccac tgtcttttat tataatttct atattatctt tttttacaat
    15541 ttctacactt atcaaagttc cttcactatt atgaactatt gaattaaata taagattggt
    15601 tatagccctt ctgaataaga ttgaatctac atgtacttca attaaatctt cattgctttc
    15661 aaactcaata ttcctatttt tatatctatt atcatttaaa atatcaatta ttatatctct
    15721 gactaaacta actaaattta tttttttctt gtccagaact atagtattat tttttaatcg
    15781 agtagataaa tttaaatcat caactaaatc ttttatatac ttagacttat tatatataat
    15841 ttctgtatac tctcgtattt catcttcttc aaactcataa tttttgtcat taattatctc
    15901 tgcataccct tgtatagatg ctagaggtgt ctttatatca tgtgttatat ttgccagcca
    15961 ttcctctctc aactcttcat tttcttttct ctcaacttca tttactctta aagtatcagc
    16021 taacatattt atatttttga aaacttcttc atatatacct ctgtccttaa gatataaact
    16081 ataatttcca ttagccatag tctcaatact ccatagtacg cttgaaattg gttttgtaag
    16141 ttttctacta aataaatatc ctacacctaa agctaccacg ctatcaatta ctagtgttat
    16201 aatgataact ttatttaaag tttttttaat gttattttga gaataggtta aaattattct
    16261 tgatatatct ctactaggta tacctactat tatgtttaga tgttttttgt ttaaatattt
    16321 ttctagtaca aaattaacat actttgtttc tatatacttg taattattca ccatttgaaa
    16381 gggtgtatat ttttttggag tatcctttgg cacatttact ccataaactt cctctcctaa
    16441 atcatttaaa acctgtatcc atgaatttga tttttctaat ttttcagctc caatcttaga
    16501 tagtttaaat tctccatctt tctcatataa atctttttca aaagagcgtg caaattcttc
    16561 aggattattt ccagaatcta caactttaaa aaaactatca gttgaaacca catacaatat
    16621 agatataata tttataatta ctactgatat aactacaaac acaatcgtaa atataaaatt
    16681 tctagtaatc ttccacttca tattctaatc ttcctttact gctaatttat atccaagacc
    16741 ttttattgtc agaatatatt caggctttga aggattatcc tctaattttt ccctaatttt
    16801 tcttatatga accattatag tattgtcaaa tccaacataa tcttctcccc acactttatc
    16861 ccatagtttt tctttactta taatttgatt ttgattatgt gcaaataaag ataggatttt
    16921 tagttccttt gcagacagtc ctatagattt tccatctttt ataagttctg ccctactttc
    16981 atttaatata tatgggccaa atgatataat tttttcttca ttattattta aatttttact
    17041 ttcattttga atatcactaa aataacttaa tcttctcaaa tgagctttaa ctctaaaagc
    17101 cacttcctta gggctaaaag gttttgtaac ataatcatct cctccaatcg ctagtccaag
    17161 taacttatct aattcttcgg ttttagctga taaaaatatt attggagaat tggaggtttt
    17221 tcttatttct ttacagatat cataaccttc gccatctggt aacataatat ccaacacaat
    17281 caaatcagga ttaatacttt taaatacttc taatccttct tctttagttt ttgcagtata
    17341 tatattgcta agaccctctt tttttaggac agtttctaaa agcttcaata tttctacctc
    17401 atcatctatt attaaaacac gattttccat aaataacctc ccgtaataat ttacatatat
    17461 attagcatat attttatc
//
LOCUS       DAEPXF010000077        17227 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain HB27
            SAMN13376389-rid12479423.denovo.081, whole genome shotgun sequence.
ACCESSION   DAEPXF010000077 DAEPXF010000000
VERSION     DAEPXF010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13376389
            Sequence Read Archive: SRR10518432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 17227)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17227)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001069624.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001069624.1
            Genome Coverage       :: 315x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/15/2021 01:54:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,165
            CDSs (total)                      :: 4,076
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 89
            rRNAs                             :: 10, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 10 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 19 of 69
            Pseudo Genes (incomplete)         :: 44 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 10 of 69
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17227
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13376389-rid12479423.denovo.081"
                     /strain="HB27"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="China: Hebei"
                     /collection_date="2017"
     gene            97..432
                     /locus_tag="KRL67_002176"
     CDS             97..432
                     /locus_tag="KRL67_002176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH1523050.1"
                     /translation="MINLFKKEDLNLLLEYPKEVVENVDNVINILDESYGDNRKITDN
                     GGYVCIIEDIKEIEYLKSHILNGLIEEFSDVIYESEVDIYNSTLYLLSSDYSITIISK
                     NEETEHLLK"
     gene            496..957
                     /locus_tag="KRL67_002177"
     CDS             496..957
                     /locus_tag="KRL67_002177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HBH1523051.1"
                     /translation="MDKKKIPAKRVDIVSLKLVKESSVLYETRKISNPYDAYRLVKNF
                     LVDSDREKFVVVCLDTKNQPVSIEIVSIGTVNSAMVHPREVFKVAVLSNASKIICFHN
                     HPSGNTNFSKEDEVITKRLQKCGEILGIELVDHIVVGDDDKYFSFKENFKI"
     gene            1098..1352
                     /locus_tag="KRL67_002178"
     CDS             1098..1352
                     /locus_tag="KRL67_002178"
                     /inference="COORDINATES: protein motif:HMM:NF017894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH1523052.1"
                     /translation="MFKKENRYMTREIAENLPIEITILLWDLIDNLDIEKDYLQIFEI
                     NPIGLGVVEIVHRQEVPEYEASIYIQNDLIKYKLKIYAIE"
     gene            1419..2015
                     /locus_tag="KRL67_002179"
     CDS             1419..2015
                     /locus_tag="KRL67_002179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH1523053.1"
                     /translation="MINNKSEIINKLFFNELKELIDKYNNIEDKTVYMIEKIFISIED
                     EEIKNYLIRNNCKLQELVNRYKKLELLNIDEAIFFAWYNLNINTISIDKASQYYYELT
                     TSNYIEVESHLVYTNEIDLREYAKDELDTMLDMEYHIDRLLDKDMIIDMWLNNTSKED
                     VIEEILMSDDIEDILDISPEYAFTTTSGIEYRYSEKEE"
     gene            2211..2426
                     /locus_tag="KRL67_002180"
     CDS             2211..2426
                     /locus_tag="KRL67_002180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mor transcription activator family protein"
                     /protein_id="HBH1523054.1"
                     /translation="MVDILGEEIVIKLHKYYRGQQITFPMKLYSNEYVERYIEKNYRT
                     KTLKDMCRELGYTEGWIKQLINKYKLK"
     gene            2655..4847
                     /locus_tag="KRL67_002181"
     CDS             2655..4847
                     /locus_tag="KRL67_002181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH1523055.1"
                     /translation="MTKFNFIGNEIYITEERNRYNSIRIEYENIANKAREEFIKVYRS
                     CNENLYDVINNAYDQGASIILKSIKCTLDRLIENKFYNISEELFIEQYCQRVVEIWES
                     AYGIINDQYMNIVLDERQKEEYRQLRKNSRARWQGGGFGIQGAIKGATQAGAMNMATG
                     VAHSAFNMISKIGTSIKVNKQKSKIFNDPSTLDVLSEVIYLAILDIKYSYIKFLENNA
                     GLQFGYIHEEEEEEANVILSNIKGRNLDEEEKINLIKGLIDLNPYMESLYTYIVDNFG
                     DENMEVSKMASYFGFNIEPYKLSLVENKLINLETNTEEETILAKQLILKEVNRLGINS
                     KVNGIEELDRKLNQFDIEARTVDNILFETREDANLARIEKEKIDLILSKINMKIEEEV
                     IRLKNDILSLKLKTGIEDSYIKMIDEKLQKFDIEARTVENLLLDTREEAEKIKLDKIK
                     VEEIINNIDETSEQSLLNAKTEIENIGIRLESTKGAIEKLNYSLKKVDEIERTVNEIL
                     FDTREEANVAKKEMLELDEILQNVDLDDEESIKVAMSKIKSHGFKTKIGDNEANKLNK
                     KLEDIDTQSKMVGDILFETREKANLARQEKLDIESIIKNLDENNEENLVSIKKEIESR
                     NFKTEIANLYIDRINNHIKNLYSDTINEAEQYEDNKTNFKSMLIGSAFIVPLGIYFFG
                     NVGIILKIVIGIFLLSAVSALFESYKKLKASKCSLKQLKKLKKSGKII"
     gene            5141..5479
                     /locus_tag="KRL67_002182"
     CDS             5141..5479
                     /locus_tag="KRL67_002182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH1523056.1"
                     /translation="MEQYSFSSELIDVMVNASNDLNRVYEKITSSGTFSGLQGTFDTL
                     YEYGLMLEDLLEVMDLSLLGGNETKEIIQNIAIDLKNISDEDNIADKALDLMGNIGEF
                     TYYIETNYSK"
     gene            5614..5859
                     /locus_tag="KRL67_002183"
     CDS             5614..5859
                     /locus_tag="KRL67_002183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH1523057.1"
                     /translation="MRSVTVEDGEERFAIEKIYVKSLKRYEVRICLYRDCKDRMNKLI
                     PRPVDISPDKLSTLILVGIKAGILDDDFKQELIKGLS"
     gene            6157..6348
                     /locus_tag="KRL67_002184"
     CDS             6157..6348
                     /locus_tag="KRL67_002184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH1523058.1"
                     /translation="MKYTLKYGLGQLKFDVKIRLEDILRLSSAGEDNDEVRLAERSRE
                     INDIIGKLYLRPSETKKKI"
     gene            6437..7348
                     /locus_tag="KRL67_002185"
     CDS             6437..7348
                     /locus_tag="KRL67_002185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH1523059.1"
                     /translation="MEKYVFSFESGNNKLFKEYWKDRKKTDIPLITTIFFRTMLRLKD
                     TYRHDLYNLWEDCNLTTEEFKENIYKSQNESIRFIKFKVEKMIEEEKERLGKSINNKY
                     KSNRKITELQNIDIDNIVKELIISDNPENKEIYQKMIIITKSFFKIIEYRRKLSLSNQ
                     TATDNLMEEVKKLYNTLPKESRYDAAYKNLRKLKEDIEFKIKEIDNYKEIYKREVIKN
                     CKLVSEYKKKEEKLKKSGYIEESEKMAKKYIDIERQFNESMRKKWIYIDMYIRRKDLE
                     IEEMDMKKYEDDNITNSMIKLAEEINS"
     gene            7528..7809
                     /locus_tag="KRL67_002186"
     CDS             7528..7809
                     /locus_tag="KRL67_002186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH1523060.1"
                     /translation="MELKTIRITFTVSSETSEDDLRKLCKDYMGDEYVVNDEGDFEIS
                     FYLNTVLGYNEVLYFMELIHMELEKSITLLGIRYKGIGGLQMKHILQEE"
     gene            7816..8946
                     /locus_tag="KRL67_002187"
     CDS             7816..8946
                     /locus_tag="KRL67_002187"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH1523061.1"
                     /translation="MIRNCSKIIKNYGFKINSKNHVFDSNGNEICNFVPIVLGQYMIG
                     DGRDRRIVIVLMGISEDGNQLETINIDVSDIEHLKWVKNSWGFQYKVHSKMKNQFLYL
                     IEVLVINGTTKKLSSQIGWVKDNGKYAYLHTRGVIGESDKEVDIGDHLSNYFLPSEIV
                     DIDRACKASLNLLEVADYEVTVPLLALVYLSPLLQAIKDVGKMPEFVVWLFGTTCSRK
                     TSLARVFLSHFGDFTNRLPATFNDTYASIEIKANAIKDSLNLIDDYAPKQTKKQKDAQ
                     DEIAEKAIRAYGDRIARGRANSSMISQKQYIPQGMLLMTGENLIRGHSTVARLVALEL
                     DKDSVNLKLLKEMQDSVSLLGESMRGYIEWLLHGMNKDSSDI"
     gene            9073..9570
                     /locus_tag="KRL67_002188"
     CDS             9073..9570
                     /locus_tag="KRL67_002188"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH1523062.1"
                     /translation="MLSYFEDKKVISEVEFNQYIQFAKDTMVNVLIKNNNITKESSPI
                     DEFLYTIKEAIDSNSIKISTLVDGNKLNDNDDDTYGYKDDKYFYFHPDKTYSYVQEIQ
                     SKSGNYISLTKRGLIKLLREHSIIKVDSDGSPSKYTIKVKNGTDYKEKRPRLLRVAIN
                     TIEKL"
     gene            9612..10055
                     /locus_tag="KRL67_002189"
     CDS             9612..10055
                     /locus_tag="KRL67_002189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH1523063.1"
                     /translation="MTIRINLNGENILENADNKFYGLRARVEFDLDLLDEDEDVIDIM
                     DRILTLDVKPSRMFITKGRVDIFYQPQKEIAIQSKSGYMKLINEFIDFIESQEIVEMK
                     FSGWNLNGDKVVESQANDSNISFSKEIFNPDTVYLDGLIEGYVNL"
     gene            10162..11079
                     /locus_tag="KRL67_002190"
     CDS             10162..11079
                     /locus_tag="KRL67_002190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH1523064.1"
                     /translation="MNYITTQGFDTIKKDKIEIKRMVYALENEGDSYNVIEECIYTLE
                     ENIDVNDDFVIEVENVFSFVINRIMDFTKERNLPKNKIEHVRAYIDVFENPTSNTKIV
                     KSIDIYEDYDKRYIDNELDKLFKSMDNMIKPEFVPVIDGKFDIRGSKDLKEYNKKLML
                     DIVKQKTYEVNNGGIRINKLSAILKLGDKSIDGISKMMKDVANLKIKPKSVYISEDTV
                     VVMYICNKSCIVLNIEEYSKILSDFVDYINNIEEMDLYLDDSILYGDYKINCGVDDRD
                     IIDFNYQSFNSKVTLFAGHIGNKIIQAAL"
     gene            11109..11264
                     /locus_tag="KRL67_002191"
     CDS             11109..11264
                     /locus_tag="KRL67_002191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH1523065.1"
                     /translation="MTETYKISIGRKDLYSKDYKAVERIDYEKRYIVNISRSLVDILS
                     ILIEEGI"
     gene            11346..12323
                     /locus_tag="KRL67_002192"
     CDS             11346..12323
                     /locus_tag="KRL67_002192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HBH1523066.1"
                     /translation="MKSKKAVVYARFSSDNQRDESIDAQLRAINEYADKNNIKIVNQL
                     IDRAKSATSDKRPEFQNMIKFCEADTTGISMVIVHKLDRFSRDKYDSAMYKQKLKVKG
                     IRVVSVLENLDNSPESLILESVIEGMAQYYSANLAREVAKGQKENALKALHNGGDAPL
                     GYDVAHDKTYTVNEEEAQAVKIIFDRYIKGYSYSKIIDELNDLGYKTKRGNKFGKNSL
                     HGILNNEKYTGVYVFNKTQRKGVDGKRNGHKQKSEDEIIKVEGGMPVIIDKEVFEQAQ
                     EMIQKRKKTPGAHKATTTYLLTGLIKCGECGHAFQGNKRKDSYGNICFI"
     gene            12301..12909
                     /locus_tag="KRL67_002193"
     CDS             12301..12909
                     /locus_tag="KRL67_002193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HBH1523067.1"
                     /translation="MVIYVSYRCGCRKQKRDCNNREIKRDYLEEFILQELEKNILNDE
                     AIPALSKALNEKLNYKNESNAELLKNLDQKLEKVNKEISNILNTIMNGIVSSMLKDKL
                     DELEQVKLNLDLKMNELKIENKVVDGVGVTEEQIRSMFSRFKEFVLERNIPECKKFIG
                     DYIKEVIVYKDHVEVVFNVVFSFVDDEIAHNLHITVSRVKVK"
     gene            13042..14106
                     /locus_tag="KRL67_002194"
     CDS             13042..14106
                     /locus_tag="KRL67_002194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4297 domain-containing protein"
                     /protein_id="HBH1523068.1"
                     /translation="MSKIEYYMNLPYDLSGSMSKNRFRNELLWGLKKMYELYKTNEDF
                     VVVFDYRCDIEVHLENKFEFYQVKTQNNSGTYTINKLTDKKKGSKSVVGNLYSLKYDI
                     NMNEIDDVYVAIVSNAPLNDGKKTYNNSHEVKIKTIDSSAIEKIKLSIKNDLNIDEDI
                     NLENLFFIRIGLDLIEPQKTLIGETAIFFEDVFGRECSKVKSLYNVLYEEIYSKSAYE
                     LDINTYENIVSKKGINRDRMDIILKSYANNTDKSVELAKYSINEWYKNNFSRRLSLIK
                     SLTTIVSKLSESSILKDIENCILSYIYNNIDKLQVSDKELIEIISEIVEEKRPIEVSK
                     TDIEALILLTLKKYEEGVYE"
     gene            14099..15772
                     /locus_tag="KRL67_002195"
     CDS             14099..15772
                     /locus_tag="KRL67_002195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH1523069.1"
                     /translation="MNSLFVNSLYIFEYESKMAKKIDFKHGINILTSDEKSGNDVGKS
                     VLLKSIYHTLGADSIFDHMWNKITKTYILNVTIKNKTYYVYRRDNLFKIYNTNFKKIF
                     MTNNRDELSNYLSDLYSFKVMLPKRENEELELAPPVYSYLLNYIDQDHLDGSKFSSFN
                     SLTQFPNYKENVIYTHFGLFDEEYYKAIKELEILKSEEKNKKKEREVTLNMLNKIKSY
                     LGNLDAPESIEVLNIELNKTKEEYSNIVINLQKIKNNLMKIRNEKVDLELNIKEIELT
                     RRNEMKKLKVKNVCPSCSHEIEDIDSKFIINNQIEDLIILKDELESEIIKCNRVIEKK
                     EEEYQSLLDKLHEYESNMKINDDSISNVLKHKAYIDTKDDIVLDLEIIDKKIKEIQQT
                     IEPYDKKIKEYNSLKKKANAKYEELMKKAKKDFGLEEIDENKIKNIKNNFIARGSNRA
                     ITTIMWYLTLLAVKYEFNENAIKFPLILDSPNNVESDEIKEESLFKYIFKNTKTDTQL
                     ILSSLGFNEMQFSEFKFDNIIKLENSKYNLLNKEDYYNNIDILNKIFEN"
     gene            15877..16839
                     /locus_tag="KRL67_002196"
     CDS             15877..16839
                     /locus_tag="KRL67_002196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH1523070.1"
                     /translation="MKDRFNLKRKSIQSLMNKNVDVYSCKDCIDDFIELNFKIYRSYY
                     ISDDDILEIMDGTGEDYKDVIADMLPDKAHIKSGEFGEITATEIYKSNIQTTEIYIVD
                     KLSHTRKEDTKVAAHKTDILIIEKTSEGFIIHSSEVKTKITDSDFNPIEAMIEGVNDD
                     IVTRVAETLNWIQRKLKTEKKFDELKFIKQVIESIENQNVENEYNGAVFIDESKLDYE
                     INKNIYCDVKLSKSQKGKYDNELKSIGCVIDGDKIRFDSVNIDDINKLNINSRKRNSI
                     EKLYNKSQRKLEHIDDIKIQIVTFDDINKHCLDMYEKIIHVGRI"
     gene            16839..>17227
                     /locus_tag="KRL67_002197"
     CDS             16839..>17227
                     /locus_tag="KRL67_002197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH1523071.1"
                     /translation="MSINNNINKIRNLDIQLAKYDCISRMSAKETSKHYMRLYFKFIK
                     LLELYEDSSLNINNGEYLNLANDLIIFSKYDMSSRFKGIDEERNLVYIAVIYYILGYR
                     FECNLILDILDMHDIENEYLKIIIYLI"
BASE COUNT         7649 a         1399 c         3044 g         5135 t
ORIGIN      
        1 ctaaagagga acatacaaaa aataacacat tgagatgttg gtagaaatac taacatctct
       61 ttttttagtt caaaaaatta taaggaggaa attaaaatga ttaacttatt taagaaagaa
      121 gatttaaact tattattaga atatccaaag gaagtagttg aaaatgtgga taacgttata
      181 aatattcttg atgaatcata cggagataac agaaaaataa cagataatgg tggatatgtg
      241 tgtataattg aggatattaa agaaatagaa tatttaaaat cacatatatt aaatggatta
      301 attgaagagt ttagtgatgt aatatatgaa agtgaggttg atatctataa ttcaactcta
      361 tatttactat ctagtgacta tagcattact ataatatcta aaaatgaaga aactgaacac
      421 ttacttaaat aagcatatac attgataaag tgtatatgct tattttaatt ttgagaaaca
      481 aaggaggaaa taatcatgga taagaaaaaa atacctgcaa aaagagttga tatagtatca
      541 cttaaactag taaaagaaag tagtgttcta tatgaaacta gaaaaataag caatccttat
      601 gatgcttata gattagtaaa gaacttttta gtagatagtg atagagaaaa gtttgtagta
      661 gtatgtttag atacaaaaaa tcaaccagtt agtatagaaa ttgtaagcat aggaactgta
      721 aattcagcga tggtacatcc aagagaagta tttaaagtag cagtattaag taatgcatct
      781 aaaataatat gctttcataa tcatccatca ggtaatacta actttagtaa agaagatgaa
      841 gttataacta aaaggctaca gaagtgtggt gaaatattag gtattgaatt agtagatcat
      901 attgttgttg gagacgatga taagtatttt agctttaaag agaattttaa gatatgagag
      961 tcttagctta aagaaattag gctaagactt tttctatgtt ctaaactaat tgaaataaat
     1021 tagttttaag ttaagcccga aaagcccttg aaaaaataat atacatataa ataattaaac
     1081 taaataggag ttgtaaaatg tttaaaaaag aaaatagata tatgacgagg gaaatagctg
     1141 aaaatctacc aattgaaata actatattac tatgggattt gatagacaac ttagatatag
     1201 aaaaagatta tctacaaata tttgaaataa atcctatagg attaggagta gttgagatag
     1261 ttcatagaca agaagtacct gaatatgaag ctagtatata catacagaat gacttaatta
     1321 aatacaaatt aaaaatatac gctatagaat agtattaaat acagtactat gattttttca
     1381 aatgaatatt aactaataat taaaggagaa cgagtaatat gataaacaat aaatctgaaa
     1441 taattaataa gctatttttc aatgaattaa aagaattaat agataaatac aacaacatag
     1501 aagataaaac tgtatatatg atagaaaaga tattcataag tatagaagat gaagaaataa
     1561 agaattacct aataagaaat aattgtaagt tacaagagtt agtaaataga tacaaaaaac
     1621 ttgaactttt aaacatagat gaagctatat tctttgcttg gtacaactta aatataaata
     1681 caatatctat agataaagca agtcaatatt actatgagtt aacgacaagt aactatatag
     1741 aagtagaaag tcatttagta tatacaaatg agatagattt aagagaatat gctaaagatg
     1801 aattagatac tatgttagat atggaatatc acatagatag attattagat aaagatatga
     1861 taatagatat gtggttaaat aatacatcaa aagaagatgt gatagaagaa atattaatga
     1921 gtgatgatat tgaagatata ttggatatat ctccagaata tgcattcaca acaactagtg
     1981 gaatagagta tagatattca gaaaaagaag aataaaataa atatgtgtgt tgaaaaaata
     2041 cgggctttaa gggcttatac cttaaagtat gtataaaaaa tacaatagat tttacaatag
     2101 aaaatcagaa tgaaactttt tctaatacta attattatta atgagtatca gaaaaggagg
     2161 tggagtaatg atcaagaata atagcgacta tcaaagggat atatgcagat atggtagata
     2221 ttctaggtga agaaatagtc attaaattac ataagtatta tcgtggacaa caaataacat
     2281 ttcctatgaa gttatactct aatgaatatg tagaaagata tatagaaaag aattatagaa
     2341 caaaaacatt aaaagatatg tgtagagagt taggatatac ggaaggttgg ataaagcaat
     2401 tgataaataa atacaaatta aaataaatac cctagttatt gttaaattat tcaataaaaa
     2461 atatgatttt attacaaaaa tttacaattt cgacaagatg aatgttgtat tatatcaacg
     2521 aaagatatta aatttattca tatcaaatat aaattatcaa actttataac taagttatct
     2581 aataagagac attagtacaa agttcattag taagtgaaca taaagaatta aggcaatgga
     2641 gagggagatt gaaaatgaca aagtttaatt ttataggaaa tgaaatttat ataacagaag
     2701 aaagaaatag gtataatagc ataagaatag aatatgaaaa tatagcgaat aaagcaagag
     2761 aagaatttat taaagtatat aggtcatgta atgaaaatct atatgatgta attaataatg
     2821 cttatgatca aggggcatca attattttaa aatcaattaa gtgtacacta gatcgactta
     2881 tagaaaataa attttataat ataagcgaag agttatttat tgaacaatac tgccaaagag
     2941 ttgtagaaat ttgggaatct gcatacggta taatcaatga ccagtatatg aatattgtat
     3001 tagatgaaag gcaaaaggaa gagtatagac aactaaggaa aaattcaaga gctagatggc
     3061 aaggtggagg ttttgggatt caaggggcta taaaaggagc tacacaagca ggagcaatga
     3121 atatggctac aggagttgca cattctgcat ttaatatgat aagtaaaata ggtacgagta
     3181 taaaagtaaa taaacaaaaa tctaaaatat ttaatgatcc atcgacatta gatgtattat
     3241 ctgaagtaat ttatttagcc attttagata ttaaatactc atatataaag tttttagaaa
     3301 ataatgcagg attacagttt gggtatatac atgaagaaga ggaagaagaa gcaaatgtga
     3361 tactttcaaa tataaaaggc agaaatttag atgaagaaga aaaaataaat ttaattaagg
     3421 gacttataga tttaaatcca tacatggagt ctctatacac atacatagta gataactttg
     3481 gtgatgaaaa tatggaagtt tctaagatgg ctagttattt tggttttaat atagagccat
     3541 acaagcttag tttagtagag aataaattaa ttaatttaga aactaacaca gaagaagaaa
     3601 caatattagc taagcagctt atattaaaag aagtaaatag attaggtatt aatagtaagg
     3661 taaatggaat tgaagagtta gaccgaaaat taaatcaatt tgatatagaa gcgaggacag
     3721 tagataacat attgtttgaa acaagagaag acgctaattt ggcgagaatt gaaaaagaaa
     3781 agattgattt aattttatct aaaataaata tgaagataga agaagaagta ataaggttaa
     3841 aaaatgatat actaagctta aagttaaaaa caggaataga agactcatat ataaaaatga
     3901 tagatgaaaa gctacaaaaa tttgatatag aggctagaac tgtagagaat ttactacttg
     3961 atacaagaga agaagctgaa aaaattaaac tagataaaat aaaggttgaa gaaataataa
     4021 ataatataga tgaaactagc gaacaaagtt tattaaatgc aaaaactgaa atagagaata
     4081 taggtattag gttagagtca accaagggtg caattgaaaa actaaattat tccttaaaaa
     4141 aagtagatga gatagaaaga acggtaaatg agatactatt tgataccaga gaagaggcaa
     4201 atgtagctaa gaaggaaatg ttggaattag atgaaatact acagaatgta gatttagatg
     4261 atgaagagag tataaaggta gctatgtcaa aaataaagtc acatggattt aaaacaaaaa
     4321 taggagataa tgaagctaat aagctaaata aaaagttaga agatatagat acacaatcta
     4381 aaatggttgg agatatatta tttgaaacaa gagaaaaagc taatttagca agacaagaaa
     4441 aattagatat agaaagtata ataaaaaatt tagatgaaaa caatgaagaa aatctagtta
     4501 gtattaaaaa agagattgaa tctagaaatt ttaaaactga aatagccaat ttatatatag
     4561 atagaataaa taatcatatt aaaaatttat attctgatac tataaatgaa gcagaacaat
     4621 atgaggataa taaaactaat tttaaaagta tgttaatagg atcagcattt attgtacctt
     4681 taggtatata cttttttgga aatgttggga ttatattaaa gatagttata ggtatattct
     4741 tattatccgc agtctctgca ttatttgaat catataaaaa actaaaggca agtaagtgtt
     4801 cattaaaaca acttaaaaag ttaaaaaagt caggtaaaat aatataatta tcatgaggaa
     4861 tcaaattgaa aggtatattt aataaaacat tgacaaagat tataataagt tcattaatat
     4921 ttggaactgt atgtagtagt atagtttatc taggtatgag aaatgaattt aacaataaat
     4981 tagtttttta gaaaaagaga tactagactt aaaaaaacaa aacgtagaat tatctaataa
     5041 agaattagaa aaagaatatg ataattcaga cgttaaatct gaagatgtta aaaaagatag
     5101 tgagattact aatgaggtaa aaaaagaatc tgaaaaagaa atggaacagt atagttttag
     5161 tagtgaatta atagatgtaa tggtaaatgc tagtaatgac ttaaatagag tatatgaaaa
     5221 aataacaagc tcaggaacat ttagtggatt acaaggtaca tttgatactt tatatgaata
     5281 tggattaatg ttagaagatt tactagaggt aatggattta tctttattag gaggtaatga
     5341 aactaaagag attattcaaa atatagctat agatttaaag aatatatctg atgaagataa
     5401 tatagctgat aaagcattag atttaatggg taatataggg gagtttacat actatattga
     5461 gactaactat agtaaataat taaatattaa taataatagc cttgctacac atgactgtag
     5521 caaggtatta tttttgtaaa gaaatgaaga tttaatttgg ctaaataaaa taaggctcaa
     5581 ctatttgaaa aagaaactaa atattgtaag ataatgagaa gcgtaacagt tgaagatgga
     5641 gaagaaagat ttgcaataga aaaaatatat gttaagtcat tgaaaaggta tgaagttaga
     5701 atatgtttat accgtgattg taaggacaga atgaataagc taataccaag accagttgat
     5761 ataagccctg ataaattatc aactttaata ttggtaggta taaaggcggg tattttagat
     5821 gatgatttta agcaagagct aataaaaggt ttatcttagg aggtatagaa tgtcgataga
     5881 agaatttact agtggagtaa tgtttggaat ttctatattt ggtacagtat taaagaagtt
     5941 attagataat aaaaaatagt acataggagg tgttattatg attggattta taataggaac
     6001 tatgatagga gttggattat gcattagcat agaaattatg atatcaaaaa atacaatagt
     6061 atattaacct atatttaaac tgttaatata atcatatata atttatttga gtccttaaag
     6121 cccagaaaag ttttatatga aggagaattt tataatatga aatatacact aaaatatgga
     6181 ttaggacaat taaaattcga tgtaaaaata aggttagaag acatattaag attgtcatct
     6241 gcaggagagg ataatgatga agtaaggtta gctgaaagat caagagaaat taatgatata
     6301 attggaaagc tatatttaag accgagtgaa actaagaaaa aaatctaata gctatgaata
     6361 aagtaaaaat aaatctaaaa gtaaatatga tagagataaa attaaggaca tattagaaga
     6421 agatgagttg aatgtaatgg aaaagtatgt attttcattt gaatcaggaa ataataagct
     6481 atttaaagag tattggaaag acagaaagaa gacagatata ccattaataa caacaatatt
     6541 ttttagaact atgttaagat taaaggatac atatagacat gatttgtata acttatggga
     6601 agattgtaat ctaacaacgg aagaatttaa ggaaaatata tataaatcac aaaatgaatc
     6661 tataagattt attaaattta aagttgaaaa aatgatagaa gaagaaaaag aaaggttagg
     6721 gaaaagtatt aataacaaat ataaaagcaa tagaaaaata acagaattgc agaatattga
     6781 tattgataat atagtaaaag aattgattat atctgataat cctgaaaata aagaaatcta
     6841 tcaaaagatg ataattatta caaaatcatt ttttaagata attgaatata gaagaaaatt
     6901 atcattaagt aatcaaacag caactgataa tttaatggaa gaggtaaaaa aactatataa
     6961 tacattacca aaagaatcga gatatgatgc agcatataag aatctaagaa agttaaaaga
     7021 agatattgaa tttaagataa aagaaataga taattataag gagatatata aaagagaagt
     7081 tataaaaaat tgtaaattag ttagtgagta taaaaaaaaa gaagaaaaac taaagaagtc
     7141 tggttatata gaagaatcag aaaaaatggc aaaaaaatat atagatatag aaaggcaatt
     7201 taatgagagt atgagaaaaa aatggatata tatagatatg tatataagac gtaaggactt
     7261 agaaatagaa gaaatggata tgaaaaaata tgaagatgat aatataacga attcgatgat
     7321 taaattagca gaagagatta actcttaatt agcataaaaa tagtacacag ttaagtgtgc
     7381 tatttttttt atgaaaatga aaaaaattgg ataatggttt ggttattttt taataaaaaa
     7441 ttagcttata tttaatttca gaaggaggta aggaaataaa aatacgggct ataagggctt
     7501 taggacaact tagtaggagg aacaaaaatg gaacttaaaa caataagaat tacatttaca
     7561 gtaagtagtg aaacaagtga agatgattta agaaagttat gtaaagatta catgggtgat
     7621 gagtatgttg ttaatgatga aggtgacttt gaaatatcct tttatttaaa tacagtactt
     7681 ggatacaatg aggtcttata ctttatggag ttgattcaca tggagctaga aaaaagtatt
     7741 actctattag gaattagata caaaggaata ggtggactcc aaatgaagca tattctacag
     7801 gaggaataga gtaagatgat taggaattgt agtaagataa ttaagaacta tggttttaaa
     7861 ataaacagca aaaatcatgt atttgatagt aatggaaatg agatttgtaa ctttgtacca
     7921 attgtattag gacaatacat gataggcgat ggaagagatc gaaggatagt tattgtattg
     7981 atgggaatat cagaggacgg taatcaacta gaaactatta atatagatgt tagcgatata
     8041 gagcatttga aatgggttaa gaatagctgg ggatttcaat ataaagtaca ttctaaaatg
     8101 aagaatcaat ttctatactt aatagaagta ttagttataa atggaacaac taagaagtta
     8161 agtagtcaaa ttggatgggt taaggataat ggaaagtatg cataccttca tactagaggt
     8221 gtgattggag aaagtgacaa agaagttgat ataggtgatc acttaagcaa ttacttttta
     8281 ccaagtgaaa tagtagatat agacagagcg tgtaaagcaa gtctaaatct attagaggta
     8341 gcagactatg aagttactgt accattatta gcattagtat atttatcccc tttacttcaa
     8401 gctattaagg atgtaggaaa gatgccagag tttgtagtat ggttgtttgg aacaacttgt
     8461 tcaaggaaaa cttctttagc aagagtattt ctgagtcact ttggagattt tactaataga
     8521 ttacctgcaa catttaatga cacctatgca tctatagaga ttaaggctaa tgcaataaag
     8581 gattcactta acttaataga tgactacgca ccaaagcaaa ctaaaaagca aaaagatgca
     8641 caagatgaaa tcgcagaaaa ggctatacga gcatatggag atagaatagc aagaggtaga
     8701 gcaaactcta gtatgattag tcaaaagcaa tatatacctc aaggaatgtt gttaatgaca
     8761 ggagagaatc ttataagagg acactcaact gttgcaagat tagtagcatt agaattggac
     8821 aaggactcag ttaatttaaa gctactcaag gaaatgcaag atagtgtaag tttgttggga
     8881 gaaagcatga gaggatatat agagtggttg ttacacggca tgaataaaga tagtagtgac
     8941 atttgaggaa ttctaataca caactttgat ttatataaag aagaattaga gaaaatagag
     9001 tatacacata caaaaggtgg tcatggtaga agtattgaat catgtgcatg gctgctggta
     9061 ggtatatcat ccatgttatc atattttgaa gataaaaaag taatatctga agttgagttt
     9121 aatcaatata tacaatttgc taaagatact atggtgaatg tattaattaa gaataataat
     9181 atcacaaaag aaagttcacc aatagatgag tttttataca cgataaaaga agcaatagat
     9241 tctaactcta ttaagataag tactctagta gatggaaata aattaaatga caatgatgat
     9301 gatacatatg gatataagga tgataaatat ttttattttc atccagataa gacatatagt
     9361 tacgtacaag agatacaatc gaaatcggga aactatataa gtttaacaaa gagagggtta
     9421 attaaattat taagagaaca ctcgataatt aaggtagata gtgatggatc tccatcaaag
     9481 tatactatta aagtaaaaaa tggaactgac tataaagaga aaagacctag gctattacga
     9541 gtagctataa atacaataga aaaattataa tacaataaga gataaatggt gatatagaaa
     9601 gagggaatta tatgacaatt aggattaatt taaatggaga aaatatatta gaaaatgcag
     9661 ataataaatt ttatggatta agggcacgtg ttgaatttga tcttgattta ttagatgaag
     9721 atgaagatgt aatagatatt atggatagaa tattaacttt agatgtaaag ccatcaagga
     9781 tgtttataac aaaaggtaga gtagatatat tttatcaacc tcaaaaggag attgctatac
     9841 aatctaagtc aggatatatg aagttaatta atgaatttat agattttata gaatcgcaag
     9901 aaattgtaga aatgaagttt tcaggatgga acttaaatgg agataaggtt gtagaatcac
     9961 aagcaaatga cagtaatatt tcatttagca aagagatatt caatccagat acagtttatc
    10021 ttgatggact tatagaggga tatgtgaatc tttagaagaa gctaagttag aagtatgtag
    10081 ttctaatagg agctgcatac ttacttaata caagattatt gaaaagttaa aaaaaggaaa
    10141 ttattatgga ggagaactta tatgaactat attacaacac aaggatttga tacaataaaa
    10201 aaggataaga tagaaataaa aagaatggtg tatgctttag agaatgaggg agattcgtac
    10261 aatgttattg aagaatgtat atatacttta gaagaaaata tagatgtaaa tgatgatttt
    10321 gtgatagaag tagaaaatgt atttagtttt gttattaata gaataatgga ctttacaaaa
    10381 gagaggaatt tgccaaaaaa caaaatagaa catgtaagag cttatataga tgtatttgaa
    10441 aatccaactt caaatacaaa aatagtaaag tctatagata tatatgaaga ttatgataaa
    10501 agatatattg ataatgagtt ggataagtta tttaaaagca tggataatat gataaaaccc
    10561 gaatttgttc cagtaataga tggtaaattt gatatacgtg gaagtaagga tttgaaagag
    10621 tataataaaa aattaatgtt agacatagta aagcaaaaaa cttatgaagt aaataatgga
    10681 ggaataagaa taaataaatt atctgcaata ttaaaattag gagataaaag catagatggc
    10741 ataagcaaaa tgatgaagga tgtggcaaat ctaaagataa aaccaaaatc agtatatata
    10801 agtgaagata cagttgttgt aatgtatata tgtaataaga gttgtatcgt gcttaatata
    10861 gaagagtatt ccaagatact gtcagatttt gttgactaca taaataatat agaagagatg
    10921 gacttatatt tagatgatag cattttatat ggtgactata aaataaattg tggagtagac
    10981 gatagagata taattgattt taattatcaa agctttaatt caaaagtaac tttgtttgct
    11041 ggacatatag gaaacaagat aatacaagct gcattataga aatatacaat cgtagagagg
    11101 atatagatat gacagaaact tataaaatca gcataggaag aaaagattta tattctaaag
    11161 actataaggc ggttgaaaga atcgactatg aaaagaggta tatagttaat ataagtagaa
    11221 gcttagtaga catactttct atacttatag aagaagggat ataaattata gatatggcta
    11281 catacttagt gtgtagcctt taatatagac ttaaattagt atatttaaac tttggaggat
    11341 ataatatgaa atcaaagaaa gcagtagtat atgcaagatt tagttcggac aatcaaaggg
    11401 atgagtcaat agatgctcaa ttaagggcaa tcaatgaata tgcagataag aataatataa
    11461 aaatagttaa tcagttaata gatagagcaa aatcagcaac atcagataaa agaccagaat
    11521 ttcaaaatat gattaagttc tgtgaagcag atacaacagg aatatctatg gtaatagttc
    11581 ataagctaga tagattttct agagataagt atgattcagc tatgtataaa cagaaattaa
    11641 aagtaaaagg aataagagtt gtaagtgtat tagagaacct agataattcg cctgaatctc
    11701 taatattaga atcagttata gaaggtatgg cacaatacta tagtgctaac ttagcaaggg
    11761 aagttgctaa aggtcaaaaa gaaaatgctc ttaaagctct acacaatgga ggagatgcac
    11821 cattaggata tgatgtagca catgataaaa cttatacagt taatgaagaa gaagcacaag
    11881 cagtaaagat tatatttgat agatatatta aaggatattc atatagtaag ataatagatg
    11941 aattaaatga tttaggatat aagactaaaa gaggtaataa atttggaaag aatagtttac
    12001 atggaatatt gaataatgag aagtatacag gagtatatgt atttaataag actcagagaa
    12061 aaggtgtaga tggaaaaaga aatggtcata agcaaaagag tgaagatgaa ataataaagg
    12121 tagaaggtgg tatgccagtt ataattgata aagaggtatt tgaacaagca caagagatga
    12181 tacaaaaaag aaagaaaact ccaggagcac ataaagctac tactacatat ttattaacag
    12241 gattaattaa atgtggagaa tgtggacatg catttcaggg gaataagaga aaagactctt
    12301 atggtaatat atgtttcata tagatgtgga tgtagaaagc aaaaacgaga ttgtaataat
    12361 agagaaataa aacgagatta tctagaagag tttatattac aagaacttga aaagaacata
    12421 ttaaatgatg aggcaatacc agctttaagt aaagcattga atgagaagtt gaattataag
    12481 aatgaaagta atgcagagtt acttaaaaac ttagatcaga aactagagaa ggtaaataaa
    12541 gagataagta atatactaaa tacaataatg aatggtatag taagtagcat gttaaaagat
    12601 aagcttgatg agctagaaca agtaaagtta aacttagatt taaaaatgaa tgaattaaag
    12661 atagaaaata aagttgtaga tggtgtaggc gtaacagaag aacaaataag aagtatgttc
    12721 tcaagattta aggaatttgt gctagagagg aacatacctg aatgtaagaa atttataggg
    12781 gactatataa aggaagtaat cgtatataaa gatcacgttg aagttgtatt caatgtggtc
    12841 ttttcttttg tcgatgatga aatagctcat aacttacaca ttactgtatc aagagtaaaa
    12901 gtaaaataaa aacattaaat ataaatgtta atttaaatga tattatggat atatttaaca
    12961 aataagtagt gttacatgat ataatatcac atgtatatga aagggataaa atttatacta
    13021 ttagtattgg tggtgaaatg aatgtcaaaa attgaatact atatgaattt accctatgat
    13081 ttatcaggat caatgtctaa gaatagattt agaaatgaac tactttgggg attgaaaaaa
    13141 atgtatgagt tatataagac gaatgaagat tttgtagtag tttttgatta tagatgtgat
    13201 attgaagttc atttagaaaa taaatttgaa ttttatcaag tgaaaactca aaataattca
    13261 ggtacatata cgataaataa acttactgat aaaaaaaaag gtagtaaatc agtagtaggg
    13321 aatttatata gcttgaaata tgatataaat atgaatgaga ttgatgatgt ttatgttgca
    13381 attgtatcaa atgctccttt gaacgatggt aaaaaaacat ataataattc tcatgaagtt
    13441 aaaataaaaa caatagatag tagtgctata gaaaagataa agttaagtat aaaaaatgac
    13501 ttaaatatag atgaagatat aaatttagaa aatttatttt ttattagaat tggtttagat
    13561 ttaattgaac cacaaaaaac tttaattggt gaaactgcta tattttttga agatgttttt
    13621 ggaagggaat gtagtaaagt aaaatcatta tacaatgtct tatatgaaga gatatatagc
    13681 aaatcagcat atgagttaga tataaatacg tatgaaaata tagtatctaa gaaaggtatt
    13741 aatagagata ggatggatat tatattaaaa agttatgcta ataatacaga taagtcagta
    13801 gaactagcaa aatatagtat aaatgaatgg tacaagaata atttctcacg tagattatct
    13861 ttgataaaat cattaactac aattgtatct aaattaagtg aaagtagtat tttaaaggat
    13921 atagaaaact gtatattgtc atatatatat aataatatag ataaattaca agtatcagat
    13981 aaagagttaa ttgaaattat aagtgagata gtagaagaaa agaggcctat agaagttagc
    14041 aagacagata tagaagctct tatcttacta acattaaaaa agtatgagga gggtgtttat
    14101 gaatagtttg tttgtgaaca gtctatatat atttgaatat gaaagtaaaa tggcgaagaa
    14161 aatagacttt aaacatggga ttaatatatt aacatcagat gaaaagagtg gtaatgacgt
    14221 aggaaaatca gtactattga aaagtattta tcacacttta ggagcagata gtatatttga
    14281 tcatatgtgg aataaaatta caaaaacata tatattaaat gttactataa aaaataagac
    14341 atactatgta tatagaagag ataatttatt taaaatatat aatacgaatt ttaaaaaaat
    14401 atttatgaca aataaccgtg atgaattatc taattattta agtgatttat atagttttaa
    14461 ggtaatgctg ccaaaaagag aaaatgaaga attagagtta gcacctcctg tatatagtta
    14521 tttgcttaac tatatagatc aggatcattt agatggatct aaattttcat cttttaattc
    14581 attgacacaa tttcctaact ataaggaaaa tgtaatttat actcattttg gactttttga
    14641 tgaagagtac tataaagcta taaaggagtt agaaatatta aaatcagaag aaaaaaataa
    14701 gaaaaaagaa cgagaagtta ctttgaatat gctaaataaa ataaaaagtt acttaggaaa
    14761 cttagatgca ccagaaagta tagaagtttt aaatatagaa ttaaataaaa ctaaagaaga
    14821 atattcgaat atagttataa acttgcaaaa aataaaaaat aatttaatga aaataaggaa
    14881 tgaaaaagta gatttagaat taaacataaa agaaattgaa ttaacaagaa ggaatgaaat
    14941 gaagaagtta aaagttaaaa atgtgtgccc aagctgttca catgaaatag aagatataga
    15001 ttctaaattt ataataaata atcaaataga agatcttatt attcttaaag atgagttaga
    15061 gtcagaaatt ataaagtgca atagagttat agaaaagaaa gaggaagaat atcaatcact
    15121 attagataaa cttcatgagt atgagtcaaa tatgaaaata aatgatgata gcatatctaa
    15181 tgtcttaaag cataaggcat atatagatac taaagatgat atagttttag atttagaaat
    15241 aatagataaa aaaataaaag aaatacaaca gacaatagag ccatatgata aaaaaataaa
    15301 agaatacaac agtcttaaga agaaggcaaa tgctaagtat gaagagttaa tgaaaaaggc
    15361 taaaaaagac tttggattag aagagataga tgaaaataaa ataaaaaata taaaaaataa
    15421 ctttatagca agaggtagta atagagcaat aactactata atgtggtatc tgacattgtt
    15481 agcagtaaaa tatgaattta atgaaaatgc aataaagttt ccactaattt tagatagtcc
    15541 aaataatgtt gaatctgacg agataaagga agaatcattg tttaaatata tatttaaaaa
    15601 tactaaaaca gatactcaat taatattatc atcgttaggg ttcaatgaaa tgcaatttag
    15661 tgaatttaag tttgataata taataaaact tgaaaatagc aaatataatt tattaaataa
    15721 agaggattat tataataata tagatatttt aaataaaata tttgaaaatt agtataggaa
    15781 tcgtgggtat gatttctgtg aatcgtctcc accattgaaa tccacgaaaa cacattgata
    15841 ttttatcaat gtgtttttta agtaataggg ggaaatatga aagatagatt taacttaaaa
    15901 agaaaaagta ttcagtcatt aatgaataaa aatgtagatg tttattcttg taaagattgt
    15961 atagatgatt ttatagaatt aaattttaaa atttatagaa gttattatat atcagatgat
    16021 gatatattag agattatgga tggtacagga gaggactaca aggatgttat agcagatatg
    16081 ttaccagaca aagcgcatat aaaatctgga gaatttggtg aaataacagc aacagaaata
    16141 tataagagca atatacaaac aacagaaata tatatagttg ataaattaag tcatacaaga
    16201 aaagaggata ctaaagttgc tgcacataaa acagatattt taattattga aaaaacctca
    16261 gaaggtttta taattcattc ttctgaggtt aagactaaga taactgattc agactttaat
    16321 cctattgaag caatgataga gggggtaaat gatgatatag ttacacgtgt tgcagagaca
    16381 ctgaattgga ttcaaagaaa actgaaaaca gaaaagaaat ttgatgaatt aaaatttata
    16441 aaacaagtaa tagagagtat agaaaatcaa aatgtagaaa atgaatacaa tggtgcagtt
    16501 tttatagatg aatcaaaatt agactacgag ataaataaaa atatctattg tgatgttaag
    16561 ctttctaaaa gccaaaaagg caaatatgac aatgaattaa agtctatagg atgtgtcata
    16621 gatggtgata agattaggtt tgatagtgtg aacatagatg atattaataa actaaatatt
    16681 aatagtcgta aaagaaattc aatagaaaaa ttatataata aatctcaaag aaaactagaa
    16741 catatagatg atattaaaat tcaaatagta acttttgatg atattaataa acattgttta
    16801 gatatgtatg agaaaattat tcatgtaggg agaatataat gagtattaat aataatataa
    16861 ataagattag gaatcttgat atacaattag caaaatatga ttgtatatca agaatgtctg
    16921 caaaagaaac atcgaagcat tatatgcgat tatactttaa atttataaaa ttattagaac
    16981 tatatgaaga tagtagttta aatataaaca atggagaata cttaaatcta gctaatgact
    17041 taataatatt ttctaaatat gatatgtcga gtagatttaa aggaattgat gaagaaagaa
    17101 acttagtata tatagcagtt atatattaca tattgggata tagatttgag tgcaacttaa
    17161 tattagatat attagatatg catgatatag aaaatgaata tttaaaaata ataatttatt
    17221 taattag
//
LOCUS       DAEPXF010000078        16830 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain HB27
            SAMN13376389-rid12479423.denovo.082, whole genome shotgun sequence.
ACCESSION   DAEPXF010000078 DAEPXF010000000
VERSION     DAEPXF010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13376389
            Sequence Read Archive: SRR10518432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 16830)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16830)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001069624.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001069624.1
            Genome Coverage       :: 315x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/15/2021 01:54:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,165
            CDSs (total)                      :: 4,076
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 89
            rRNAs                             :: 10, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 10 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 19 of 69
            Pseudo Genes (incomplete)         :: 44 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 10 of 69
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16830
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13376389-rid12479423.denovo.082"
                     /strain="HB27"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="China: Hebei"
                     /collection_date="2017"
     gene            180..992
                     /gene="modA"
                     /locus_tag="KRL67_002198"
     CDS             180..992
                     /gene="modA"
                     /locus_tag="KRL67_002198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HBH1523072.1"
                     /translation="MFKKLSVLALAMIISLSITACNSKKNKANDESNTKVTTADDSKE
                     KETLFVYSGAGLKKPMDEIAQKFEKENNVKVEYSYAGSAQLIAQIETSHKGDVFIVGS
                     EPIYSKAFEKELVGEHKTVAHHTPAIVVPKGNPANIQKLEDLNKDGVKLILGDKESNA
                     IGKTTQKILEKNNLKSINDNVVSTAATVNEMIVQLTSSKADATIATKDSVFGNEDVEV
                     IEIAQDKNIDQLISAGIVNYSDKKDLANKFINYIESDEAKQIFKKYGFEPVK"
     gene            1002..1793
                     /locus_tag="KRL67_002199"
     CDS             1002..1793
                     /locus_tag="KRL67_002199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBH1523073.1"
                     /translation="MKNKFFTILLTGFFAIVFLFIVSPLLMLIIKGISYVPICLKSVE
                     VQYAITLSIKTSLISTIVCLLLAIPVAYFLHITKLPFKKLIIQIINLPMSLPHLVSGI
                     ALLLLFGRMGIGDSIYKILKLDFVFTKQGIILAQVFVNLPLTIKILHTSLNESNEKMI
                     FVARTLGCNSWEAFRFIILPNLKTGIISATVMTWSRALGEFGAVAMIAGSTRMKTEII
                     PTSIYLNMSTGDIDIAIGIAVILIFISLTCLMLFEIFFNRGVDKN"
     gene            1795..2490
                     /locus_tag="KRL67_002200"
     CDS             1795..2490
                     /locus_tag="KRL67_002200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBH1523074.1"
                     /translation="MLKIKNLSLDLPNFKLQENSLNINNHEYFVLLGASGSGKTLFLE
                     TLAGRYPSAKGNVSYKENLISNLPPENRNIGFVYQNFELFPNMNVEDNIRFPLKLRKI
                     PIDEQILKANHLSKLMHIEDIKSRYPKNLSGGEKQRVAFARALIAEPDILLLDEPMSS
                     LDYITKKHISKILKDVYIKYKPTVIHVTHDISEAMFFAHKIGIMNNGKINHIFDLNEE
                     IKNKGESFFYEYI"
     gene            2477..3208
                     /locus_tag="KRL67_002201"
     CDS             2477..3208
                     /locus_tag="KRL67_002201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH1523075.1"
                     /translation="MNIFDILRDYLLVQVDKYNLNMDMISIVSKSLSSKEAIGNTKRK
                     DFPIIVGKEIMLEADFKGAKGQAFTSTPSTFEGSLKDILSLDLYDNPHDRSLFIASLN
                     AVMKYLDKTDRTIHCKNNEPEVCAKKFPKFIKREFGNPKVAIIGYQPAIIDNIKDFFE
                     TRVLDLNPEFVDTIQYNVKIEDGIRDYEDVISWADLVICTGSTLCNNSIINFLSLNKP
                     VYYYGTTIAGASNILGLKRLCFCSK"
     gene            3522..4322
                     /locus_tag="KRL67_002202"
     CDS             3522..4322
                     /locus_tag="KRL67_002202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HBH1523076.1"
                     /translation="MTFKNKKFTINKYEILSIILVLCLILTGFSSYVNYSHKKFKDYV
                     VFYVQHQDDEVLWAGSAIVNAIKERGKNNVFVVLVSTGCGISVFDKYKKYENLTNIQK
                     SEYRNREFLASLNSLGVIRKNIILLPEINTNGNTDFNYMEKIALTFEEKFKNVTHIAH
                     TYKLDDHLQHLKNGSVIQNLYNAGKIKDAKYFVKPKFTDKITFNNKIVYKAHSTEDYE
                     KVKNACQQYKIVDELEHREGIGYKSDHKSFDKLLSNKDVPAILHTPNL"
     gene            4872..5588
                     /locus_tag="KRL67_002203"
     CDS             4872..5588
                     /locus_tag="KRL67_002203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBH1523077.1"
                     /translation="MNQNILVVDDDREIVDAIEFYLRPDNFNVLKAYDGLEALDILLE
                     NDIKLIIMDVMMPNLDGLKTTLKIREKRNIPIILLSAKSEDMDKIIGLNMGADDYITK
                     PFNPLELTARVKSQLRRYINLGDFSNTNSSNDTNYILKSGGLELNTDTKIVSLDGEEI
                     KMTPLEYKILTLLMSRKGKIFSSREIYENVWNEDAYSCERTIAVHIRRIREKIEINPK
                     EPKYLKVVWGIGYKIEKLNY"
     gene            5557..7149
                     /locus_tag="KRL67_002204"
     CDS             5557..7149
                     /locus_tag="KRL67_002204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HBH1523078.1"
                     /translation="MVTKLKNLIINDSKAKFILLLLLFLLVSILFEAVYDLNNNFSYN
                     INYYYKESALPYFYFFGTPVHLSKVELEFVCIGIILSSFLLTILCFTLYKKNKQTKVK
                     FLENVISLFSKVKIELTLILILMLLALRFHAFTSNKMFYNTNLIIYNFIIISIVYIIY
                     ENIKYNNYNPKDMSITYVFFSNLLSMYNRKTINKKLAIVFLISIVIQAIIQYLVLIFF
                     MYIYYAEAIAIVILFTLVNTVFHFYIYNFLSVKFNYINNISKNIKNIENGNLKYKLEV
                     IGDDEISTLAKSINNITGGLETAVDANIKNERMKAELITNVSHDLKTPLTSILSYVDM
                     LKNNDFDRETINDYINILDKKSQRLKLLIDDIFEASKLSSGDVEFNITKTDIRELLIQ
                     SIVEFEDKIQNSSLDFIIETPEYAVFTMIDGKKTWRVFENLICNILKYSMPNTRVYID
                     MFIKEESIILTFKNISNDKLNLKPEELIERFRRGDISRKTDGSGLGLSIAQNIIELEN
                     ANMEIIIDADLFKVMLTFKRVN"
     gene            complement(7461..8963)
                     /locus_tag="KRL67_002205"
     CDS             complement(7461..8963)
                     /locus_tag="KRL67_002205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HBH1523079.1"
                     /translation="MGDLYKLQNGTDIRGIAYENDSKEVNLTVEEVRKIAKAFHIWLK
                     EKTKKDKVTVAIGTDSRITGSQFRSTVIETLTNDDCNVIDCEIATTPAMFMTTIMDGY
                     NSDGSIMITASHLPYYYNGLKFFTESGGLEKTDIKEMLDIAVKNDSVDYEEISKKGEI
                     IVKNLIEDYSNLLIDKIRRGVNSSKNYEKPFSGLKILVDAGNGAGGFFAEKVLHILGA
                     DTTGSQFLNPDGMFPNHIPNPENKEAMESICKAVLDNKSDLGIIFDTDVDRAAIVGKN
                     GKPINKNALIAVISSIVLEEHPKTAIVTDSITSEGLAKFINELQGRHHRFKRGYKNVI
                     NEAIRLNSEGEECHLAIETSGHAALKENYFLDDGAYLIAKILIKVAKLSLEGKTIEEL
                     IENLEEAQEEKEVRIGINKEDFRPYAEDILKDLDSHVKSIDGWSVAPNNFEGIRVNCD
                     ESNGDGWFLLRISLHEPLLALNIESNKIGGAEQIYSKLKLFLEKYDLKGL"
     gene            complement(9073..9738)
                     /locus_tag="KRL67_002206"
     CDS             complement(9073..9738)
                     /locus_tag="KRL67_002206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HBH1523080.1"
                     /translation="MSIICASIMCADQMKLKEELEALEEAGVKLLHCDVMDGIFVKNL
                     AMGPELLKSISENTTIPLDIHLATETPDKYIDMMSYIKPKYISFHVESSTNVKADIQK
                     LRNYGIGPVLAISPQTSVDKIEEYISLVEGILVMTVNPGFAGQKFNLSVLDKLDKLTE
                     ILKDYDNPPFIEVDGNINKDTIKLMNGKKVDIYVVGTSALFNDKPPISYKDKIEELKE
                     SIK"
     gene            complement(9768..10214)
                     /gene="rpiB"
                     /locus_tag="KRL67_002207"
     CDS             complement(9768..10214)
                     /gene="rpiB"
                     /locus_tag="KRL67_002207"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 5-phosphate isomerase B"
                     /protein_id="HBH1523081.1"
                     /translation="MKISIGCDHGGYELKEYIKENLINKGIEVEDVGTNSKESVDFPI
                     YAKKVSQNVQSKKSDLGILCCGTGIGMSIAANKFKGIRAAVVSDCFSAKATREHNNTN
                     VLCLGERVIGKGLAMRIVEEWLNSSYNGERHARRLQMIDDIEKENL"
     gene            complement(10389..11309)
                     /locus_tag="KRL67_002208"
     CDS             complement(10389..11309)
                     /locus_tag="KRL67_002208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase family protein"
                     /protein_id="HBH1523082.1"
                     /translation="MGIATREAYGQVLKELAENKDIVVLDADLGKATKSISFKEVAPD
                     RFFDMGIAEGDMIGTAAGLATCGKIPFASTFAIFAAGRGYEQIRNSVAYPNLNVKIAA
                     THAGVTVGEDGGSHQAIEDISLMRGIPNMVVLNPADALEARQAILASIDYNGPVYIRL
                     GRAATPDVNSENYKFEIGKGTVLREGSDITVIATGIMVAKALEAAEELAKEGINVEVV
                     NISTIKPLDEALIKETAKKTGKVVTAEEHSIIGGLGSAVCEALAETKDVVVRRIGVKD
                     VFGQSGTPADLLKHYGLTTEDIVKNIKELL"
     gene            complement(11312..12124)
                     /locus_tag="KRL67_002209"
     CDS             complement(11312..12124)
                     /locus_tag="KRL67_002209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HBH1523083.1"
                     /translation="MSTLRDHSNNIRKNIIKMVAEAKSGHPGGSLSIADMLTVLYFDK
                     MNVCAENPKMADRDRFVLSKGHAAPALYATLAEKGFFPEEELITLRKFGSKLQGHPDM
                     KKVAGIDMSTGSLGQGLSAANGMALAGKLDNKDYTVYTILGDGEIQEGQIWEAAMTAS
                     HYKLDNLIAFVDLNGLQIDGSNEEVMNVNPVDDKFKSFGWNVIVINGHSFEEIGNAVD
                     EAKKVTGKPTVIIAKTVKGKGVSFMENNAAWHGTAPNAEQAEQALKDLEGGL"
     gene            complement(12216..13247)
                     /locus_tag="KRL67_002210"
     CDS             complement(12216..13247)
                     /locus_tag="KRL67_002210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012986518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HBH1523084.1"
                     /translation="MKAVVKTKPGYDNVEVLEVEEPKATGDKVKIKVEYSGICGSDIH
                     SFKGEYANIKAPVTLGHEFSGIVVEVGEDVKNIKVGDRVTSETTFATCEKCIYCETKD
                     YNLCSTRKGIGTQVNGSFAEYVLSREESIHVLPENVSLLSAALTEPLACCVHAALEKT
                     TIESSDTVLIIGPGPIGLLLAQVVKSQGATVIMSGVTKDKERLEIAKNLGVDRTVNVM
                     EESLADVVNEMTDGNGVNKGFDCSGFMPAVNEALRLLRKKGTFVQVGIFAKKLNEMDQ
                     EAIIQRELQYIGSRSQKPSSWITALDLMASGKVNTEALVTRTVGLDEFKEGITALMNG
                     EEIKVAVKS"
     gene            complement(13247..14299)
                     /locus_tag="KRL67_002211"
     CDS             complement(13247..14299)
                     /locus_tag="KRL67_002211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HBH1523085.1"
                     /translation="MKAAVLHGTNDMRFEDIEIKPCESDEVKIKVMAAGICGSDPPRV
                     LKHWKYPVPAIPGHEFSGVIAEVGKDVKNVKVGDRVVAIPFIPCNECEYCKRGLFSLC
                     DDHGMLGAKSFGAFAEYANIKATNVLPIGDMDFEDAAMIEPLAVAMHGVLNIGVQVGD
                     TVAVMGSGTMGQLVIQGLKIAGAGTIIAVDISDNKLRESKELGADIIINAKDINPVEK
                     IKELTGGKGVDIALECAGSKITQEQCLLITKKKSKIGFLGIAYSDITLSEEAFENIFR
                     KELELKGFWNSYSAPFPGQEWTKGINLVNEGKIKLKEMVSHRFSLEDTYKAFEMIRDR
                     KEEFNKILILPQGVEK"
     gene            complement(14429..15703)
                     /locus_tag="KRL67_002212"
     CDS             complement(14429..15703)
                     /locus_tag="KRL67_002212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012951111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HBH1523086.1"
                     /translation="MDKLLAVVQYILNMGPTVILPITIVVLGMIFRIKFTKALKSGLT
                     IGIGFVGINLVVNLLTSTLGPAAQGMVERFGLNLTVIDVGWPTTASATWASPVAPILI
                     PVCLLVNLLLLYFNKTKILNVDIWNYWHFIVAGAVGYIVTGSYVWAIFCAILMELLVL
                     FIAEKTAKNVQEFYDLEGIALPTGSTASFAPLGYLVGKFVEKIPGINKIQADPDSIQK
                     RFGIFGEPMMMGIILGIILGALAGYDVAGIFNVGISMGAVMLLMPRMVRILMEGLIPI
                     SESIRDFLQAKYGDRELYIGLDAAVATGHPAVISTALILVPITLFLAVILPGNKVLPF
                     GDLATIPFYMAFIVCFRKGNIVQSVITGTIVIAISLYMATNFADVHTQLLQQANMMPE
                     GTTMVTSIDTGGNLLKWGILKLSQLVSSFGIF"
     gene            complement(15762..16037)
                     /locus_tag="KRL67_002213"
     CDS             complement(15762..16037)
                     /locus_tag="KRL67_002213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBH1523087.1"
                     /translation="MKKILVACGAGIATSTVVCDKVERLVKENNIQAEVIQCKIAECA
                     TKQEGADLIVSTTILPTTYNIPAIKATAYITGVNTAALDKKILDALK"
     gene            complement(16098..16556)
                     /locus_tag="KRL67_002214"
     CDS             complement(16098..16556)
                     /locus_tag="KRL67_002214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBH1523088.1"
                     /translation="MEFINFVSDKVIDLKMDCKTKEDFFKEIHNKVFDLGFVKEEFGE
                     KILARENVFPTGLNLGDYGVAIPHTDAEYIKEQFIAVCTFNEPVVFSSMEDQDEKVPV
                     NLAFVLGLNQPHSQLSVLTELMGIMQNKELVDKLMSSTDKEEVLKTLKSL"
BASE COUNT         5667 a         2697 c         2062 g         6404 t
ORIGIN      
        1 ataaaaatta aaaataaaat aactacctat ggaaatgaca ttataaatag attttctctt
       61 aactacacat aacaaaacat aacaaaacat tataaaacat aacaaaaact tagacatcgt
      121 tttcttagta tgatatacta aaaaagaatt tacactaatt tactaagggg gaatatctta
      181 tgtttaaaaa actttcagta ctagctttag ctatgattat ttcattaagt attactgcat
      241 gtaattctaa aaaaaacaag gctaatgatg aatctaatac taaagtgaca acagctgatg
      301 attcaaaaga aaaagagact ctttttgtct attcaggggc aggtttaaag aaaccaatgg
      361 atgaaatagc acaaaaattt gagaaagaaa ataatgtcaa agttgaatat agctatgcag
      421 gttctgcaca gctaatagct caaattgaaa caagtcacaa aggagacgta tttattgttg
      481 gttctgaacc aatttattca aaggcctttg aaaaagaact tgtaggtgaa cataaaacgg
      541 ttgcacatca tacacctgca atagttgtac ctaaaggaaa tccagctaat attcaaaaat
      601 tggaagactt aaataaagat ggagttaaac ttattttggg agacaaagaa tctaatgcta
      661 taggcaagac tacacaaaaa atacttgaaa aaaataattt aaaatctata aatgataatg
      721 ttgtctctac agctgcaact gtaaatgaaa tgattgtaca gttaacatca agtaaagctg
      781 atgctacaat agccacaaaa gattcggtct ttggcaatga agatgtagaa gttatagaaa
      841 ttgcccaaga taaaaatata gaccaactca tatcagcagg aatcgtaaac tatagtgata
      901 aaaaagattt agctaataaa tttataaatt atatagagtc agacgaagct aaacagatat
      961 ttaaaaaata tggttttgaa cctgttaagt agtgattaaa cttgaaaaat aaatttttta
     1021 ctatactact tactggattt tttgctatag tctttttgtt tattgtatca ccacttctta
     1081 tgttaatcat taaagggata agttatgttc ctatatgctt aaaatcagtg gaagtacagt
     1141 atgcaataac acttagtatt aaaacatctt taatatctac tatagtatgc cttttattag
     1201 ctattccagt agcatatttt ttacatatta ctaaattacc atttaaaaaa ctgattatac
     1261 agataataaa tctccctatg tctcttcccc acttagtatc aggaattgct cttttacttt
     1321 tgtttggaag aatggggatt ggagattcca tttacaagat tcttaagctt gattttgtat
     1381 ttacaaagca aggtataatt ttagcacaag tatttgtaaa tcttccactt actattaaaa
     1441 tacttcacac ttcactaaat gaaagtaatg aaaagatgat atttgttgca cgtacccttg
     1501 gatgtaatag ttgggaagca tttagattta taattcttcc aaatctaaaa actggaatca
     1561 tatctgcaac tgtaatgact tggtcaagag cacttggtga atttggtgca gtagctatga
     1621 tagcaggttc tacacgtatg aaaactgaaa ttattcctac atctatatat ttaaatatgt
     1681 ctactggtga tatagacatt gcaatcggaa tagctgtaat tttgatattt atctcactta
     1741 catgcttgat gctttttgag atatttttta atagaggagt tgataaaaat taatatgtta
     1801 aaaataaaaa atttatctct tgatttacct aattttaaat tacaagaaaa tagtttaaat
     1861 attaataatc atgaatactt tgttttatta ggagctagtg gctcaggtaa aactctattt
     1921 ctagaaacat tagcaggaag atatccatct gctaaaggaa atgtatcata caaagaaaat
     1981 ttaatatcaa acctacctcc agaaaataga aatataggat ttgtatatca aaattttgaa
     2041 ttatttccaa atatgaatgt tgaagataat ataaggtttc cacttaaatt aagaaaaata
     2101 cctatagatg aacagattct taaggctaac cacttatcta aactaatgca tatagaagat
     2161 ataaaaagta gatatcctaa aaatttaagt ggaggtgaaa agcaacgagt tgcttttgca
     2221 cgtgccttaa tagctgaacc tgatatatta cttttagatg aacctatgag ttcattggat
     2281 tatattacaa aaaaacatat aagtaaaatt ttgaaggatg tctacataaa atacaaacca
     2341 acagtgattc atgttactca tgatatttct gaagcaatgt tttttgctca taagatagga
     2401 ataatgaaca atggaaaaat aaatcatatt tttgatttaa atgaagaaat aaaaaataaa
     2461 ggagaatctt ttttctatga atatatttga tattttgaga gattatctat tagtacaagt
     2521 cgataaatat aatttaaata tggatatgat aagtattgta tccaaatctc tatcttcaaa
     2581 agaagcaatt ggaaatacta agcgtaaaga tttcccaatt atagttggta aagaaataat
     2641 gcttgaagct gactttaaag gagctaaagg acaagcattt acttctactc catctacatt
     2701 tgaaggttct ttaaaagaca tattatcatt agatttatat gacaatcctc atgatagaag
     2761 tctttttatt gcatcattaa atgcagttat gaaatactta gacaagacgg atagaaccat
     2821 tcattgtaaa aataacgaac cagaggtatg tgcaaaaaaa tttcctaagt ttataaaaag
     2881 ggaatttggt aatccaaaag tagcaattat aggatatcaa ccagctataa ttgataatat
     2941 taaggacttt ttcgaaacaa gagtattaga tttaaaccct gaatttgtgg atactattca
     3001 atataatgtt aagattgaag atggtatacg agattatgaa gatgtaatat cttgggctga
     3061 tttagtgatt tgcacaggaa gtacattatg taataactca attataaatt ttctatcact
     3121 taataaacca gtttactatt atggcactac gatagctggt gcatcaaata ttttaggttt
     3181 aaaaagactc tgtttttgta gtaaataagc aatcacatca tcaaggagta cacaaatata
     3241 tatgtgtact ctttaaaatt aaaaagttat tacttttatt ctaagtacta aagtaaaatt
     3301 ctattaaaat gaatcccgaa taaatgtaca atccctttaa atctcaattc ctatcaatca
     3361 ttaatatttt gattagtctt atacacaaat attttcttta ttttaaacta ataaaaaaat
     3421 tttattccat taatctactc tatatttctc agcatttaag aaataatttt tagtactttg
     3481 tttgataata taaataagac aacttttcag gagatgatta aatgacattt aagaataaaa
     3541 aatttactat aaataaatat gaaattctat ctattattct agttttatgt ttgattttaa
     3601 ccggttttag tagctatgtg aattatagtc ataaaaaatt taaagattat gttgtctttt
     3661 atgttcaaca tcaagatgat gaagtgttgt gggctgggag tgctattgtt aatgctataa
     3721 aagaaagagg aaaaaataat gtatttgtgg ttttagtatc tactggatgt ggtatatctg
     3781 tctttgataa atataaaaaa tatgaaaacc taacaaatat acaaaaatca gaatatagaa
     3841 atagagaatt tttagcttct cttaatagcc ttggtgtaat aaggaaaaat ataatattat
     3901 taccagaaat aaatacaaat ggtaatactg acttcaatta catggaaaaa attgctctta
     3961 catttgaaga aaaatttaaa aatgtaactc atatagcaca tacttataag ttagatgacc
     4021 atttacaaca tttaaaaaat ggttctgtta tccaaaattt atataatgct ggtaaaataa
     4081 aagatgctaa atattttgta aagcctaaat ttactgataa aataactttt aataataaaa
     4141 tagtttataa agctcattct actgaagact atgaaaaggt taaaaatgcc tgtcaacaat
     4201 acaaaattgt tgatgaatta gagcatagag aaggtattgg ctataagtct gaccataaat
     4261 catttgataa gttattaagt aataaagatg ttcctgctat acttcataca cctaatttat
     4321 aagaaatatt tattctttta gtttaagtta taagaaggaa attatttgag attctgtgat
     4381 atagcacttt ttgtgctata ctactttttc tcactcaact attactcata tctaatttca
     4441 tcatctctta aaatatataa aagtatcatc attaaaacct ataagccttt ttaaatgctt
     4501 tatacatatc tacattaaac tacctattta cattaaacta cattaaacta cattaaaata
     4561 aatatttgtt ataaattctt aataattatg aaactcattt gttatttata tttgttaata
     4621 tatgtttaga cctaaaaaat taataaaccc aaaataacat aaggagtgtg ataaattatc
     4681 ctagatattt tcataaactt ttaacttcag attaatatgg atgtctatac ttttaaattt
     4741 gctatatgca ttgacaatat attaaaaata ctagttttta tttcatagtg aatacgctta
     4801 ctgttttcta atacctcttt ttggtataaa ataaaaattg gttaaaaata aaatttcaag
     4861 ggggtaataa attgaatcaa aatatactgg ttgtagatga tgatagagag atagttgatg
     4921 ctatagagtt ctatttgaga cctgataact ttaatgtttt aaaggcatat gatggacttg
     4981 aagcattaga catactactc gaaaatgata ttaagttaat aataatggat gtaatgatgc
     5041 ccaatttaga tgggcttaaa actacactta aaataagaga aaaaaggaat ataccaataa
     5101 ttctcttatc tgcaaaatca gaagatatgg acaaaattat aggtcttaat atgggtgctg
     5161 atgactatat aactaaacca tttaatccac tagaacttac tgctcgagta aaatctcaac
     5221 ttagaaggta tataaattta ggtgatttta gtaatacaaa tagctcaaat gatactaatt
     5281 atattctaaa aagtggtgga ctagaactaa atacagatac aaaaatagtt tctttagatg
     5341 gtgaggaaat taaaatgaca ccacttgaat ataaaatact tacacttctt atgtcacgaa
     5401 aaggtaaaat tttctcttct cgtgagattt atgaaaatgt ctggaatgag gatgcttata
     5461 gttgtgagag aactattgct gttcatatta gaagaattag agaaaaaatt gaaattaatc
     5521 caaaagaacc aaaatattta aaggtggtgt ggggcattgg ttacaaaatt gaaaaactta
     5581 attattaatg attctaaagc aaaatttatt ttattattat tactgtttct attagtttct
     5641 atattatttg aagcagttta tgatttgaat aataactttt cttataatat taattattat
     5701 tataaagagt ctgctctacc ttatttttat tttttcggta ctcctgtaca tttgtcaaaa
     5761 gtagaattag agtttgtctg cataggtatt atattatcaa gttttttatt gactatacta
     5821 tgttttacat tgtataaaaa aaataaacag accaaagtca aattcttaga gaatgtcata
     5881 agcctttttt ctaaggtaaa aatagaactt acacttatat taattttgat gctattagcc
     5941 ttaaggtttc acgcatttac aagtaataaa atgttttata acactaattt aataatctac
     6001 aattttataa ttatatctat agtctacata atatatgaaa atattaaata taataactat
     6061 aatccaaaag atatgtctat aacatatgta ttcttttcaa acttacttag tatgtataat
     6121 agaaaaacta taaataaaaa attagctata gtatttttga tatctatagt tatacaagct
     6181 attatccaat atctggtttt gatattcttt atgtatatat attatgctga ggcaatagct
     6241 atagtaattt tatttacact tgtaaacaca gtttttcatt tttatatata caacttttta
     6301 tctgttaagt ttaattacat aaataacata tcaaaaaata taaaaaatat agaaaatggc
     6361 aatttaaagt ataaattaga agttataggg gatgatgaaa tatctacttt agcaaaaagt
     6421 ataaataata taacaggtgg tcttgaaact gctgtagatg ctaatataaa aaatgaaagg
     6481 atgaaggcag aacttatcac aaacgtatct catgacttaa aaactccact tacatctata
     6541 ttaagctatg ttgatatgtt aaaaaataat gattttgaca gagagactat aaatgactat
     6601 ataaatattt tagataaaaa atcacaaaga ttaaaattat taatagatga catatttgaa
     6661 gcatctaagc taagtagtgg agatgtagaa tttaatataa caaaaaccga tataagagaa
     6721 cttttaattc aaagtattgt agagtttgaa gacaaaattc aaaatagcag tttagatttt
     6781 ataatagaaa cacctgaata tgctgttttt accatgatag atggaaaaaa aacttggaga
     6841 gtttttgaga atctaatttg caatatacta aaatactcta tgcctaacac tagagtctat
     6901 atagatatgt ttataaagga agaaagtata atattaacgt ttaaaaatat ttcaaatgat
     6961 aaactaaatc taaagccaga agagttaata gaaagattta gaaggggtga tatatctaga
     7021 aaaacagatg gctctggtct tggtttatca attgctcaaa atataattga attagaaaat
     7081 gcaaacatgg aaataattat agatgctgat ttatttaagg taatgcttac ttttaaaaga
     7141 gttaattgat atattaatat aaaaatatat tttaagttat ttaaacaaga aaaaaacctc
     7201 cttagaggtc tttttctaaa tgttggggtt tatatttaca tattttttga ttataccttt
     7261 ttggtgtgat attaatacat tttaaattat atttctctat aattcatcac atatttcatc
     7321 tctctgtatc tataatatca catagtgttt ccataatcag ttacatctct gttacctaag
     7381 attacaattt tgtaatattt gtaatcttag gtataaattt acatttattc ttcaatataa
     7441 atatattctt tctatttacc ctaaagacct tttaaatcat atttttctaa gaaaagtttt
     7501 aacttagaat aaatttgttc tgctccacca attttatttg attcaatatt taaagctaat
     7561 aatggttcat gtagtgatat tctaagtaaa aaccatccat ctccatttga ttcatcacaa
     7621 ttaactctta tgccttcaaa attatttggt gctacagacc atccatcaat actcttaaca
     7681 tgagaatcta aatcttttaa tatatcctct gcatatggtc taaaatcttc tttattaatg
     7741 cctattctaa cttctttctc ttcttgagct tcttcaaggt tttctatcaa ttcttctata
     7801 gtctttcctt ccaaggataa ctttgctact tttattaaaa ttttagctat taaatatgcc
     7861 ccatcatcta agaaataatt ttcttttaaa gcagcatgtc cagacgtttc tatagctaaa
     7921 tggcactctt caccttcact attcaatctt atagcttcat ttattacatt tttatatcct
     7981 cttttaaatc tatgatgtct tccttgtaat tcatttataa atttagcaag tccttcagaa
     8041 gtaatcgaat ctgttactat agctgttttt ggatgttctt ctaatactat tgatgatata
     8101 acagctataa gagcattttt atttattggt ttgccatttt tacctacaat agcagctctg
     8161 tctacatcag tatcaaagat tattcctagg tcgctcttat tgtccaacac agctttacat
     8221 atgctttcca ttgcttcttt gttttcggga ttaggtatat gatttgggaa cataccatct
     8281 gggttcaaaa actgactgcc tgtcgtatct gctcccagta tgtgtaaaac tttttcagca
     8341 aagaatccac cagctccatt tcctgcatca accaatatct taagcccaga aaaaggcttt
     8401 tcataatttt tacttgaatt tacacctctt ctaattttat ctatcaataa attagaataa
     8461 tcttctatta aattttttac tataatttca ccttttttac ttatttcctc atagtctaca
     8521 ctatcatttt taacagctat atccaacatc tcttttatat cagttttttc taagcctcca
     8581 ctttctgtga aaaactttaa accattataa taataaggta agtgacttgc tgtaatcata
     8641 atcgacccat cagaattata tccatccata atagttgtca taaacatagc tggagttgtg
     8701 gcaatctcac aatcaattac attacaatca tcattagtta aagtttcaat aacagtactt
     8761 ctaaattggc ttcctgttat tctagaatca gttcctatag caacagttac tttatctttc
     8821 ttagtctttt cttttagcca aatatgaaac gctttagcta tttttcttac ttcttctaca
     8881 gttaggttaa cttctttaga atcattttca tacgctattc ctctaatatc agtaccattt
     8941 tgcagtttat ataggtctcc cataaaaaat tctccttact caatcaaaat ttttattaaa
     9001 tattataaaa tttcttgttt ctagctaaac ttttacgatt aaacagaaat atattataat
     9061 tttattacat ggttacttta tactttcttt tagttcttct attttatctt tatatgaaat
     9121 tggaggcttg tcattaaata aagctgaagt accaacaaca tatatatcta cttttttacc
     9181 attcataagt ttaatagtat ccttatttat atttccatca acctctataa atggaggatt
     9241 atcataatct tttagtatct ctgtaagttt atccaactta tccaatacag aaagattaaa
     9301 cttttgacct gcaaaccctg gatttactgt cattactaag ataccctcaa ctaaacttat
     9361 atattcttct attttatcca cacttgtttg agggcttatt gctaacactg gccctatacc
     9421 ataatttctt aacttttgta tatcagcttt tacatttgta cttgattcta catgaaaaga
     9481 aatatatttt ggctttatat aagacatcat atcaatatat ttatctggag tttctgtggc
     9541 taagtgtatg tctaaaggta ttgttgtatt ttcacttatt gatttcaata actcaggtcc
     9601 catagccaag tttttaacaa atattccatc cataacatca caatgaagta attttacacc
     9661 agcttcttca agagcttcta attcctcttt taacttcatt tggtctgcac acattattga
     9721 agcacaaata atagacataa cgttccccct tgttataaat tatatattta taaattttct
     9781 ttttctatat catcaatcat ttgtaatctt cttgcatgtc tttctccatt ataacttgaa
     9841 tttaaccatt cttcaacaat tctcattgct aatcccttcc caataactct ttctcctaaa
     9901 caaagtacat ttgtattatt gtgttctcta gttgccttag ctgaaaaaca gtcagaaaca
     9961 acagctgctc tgattccttt aaacttattt gctgcaatag acatacctat tcctgttcca
    10021 cagcaaagta tacctaaatc agatttttta ctttgaacat tttgacttac cttttttgca
    10081 tatattggaa aatctacact ttcttttgaa tttgtaccca catcttcaac ttctatgcct
    10141 ttatttatta aattttcctt tatatattcc tttaattcat aaccaccatg gtcacatcca
    10201 atagatattt tcattaaata gcacctcttt ttataatttt ttttattact ttcatactat
    10261 tttataatta tcataaaaat aaagtattgt aaaaataaag ctgtcccatt gtagaaattt
    10321 ctttctattt ttaagacagc tttaattgac ttatgtcaaa tcaatttgtt atttagatat
    10381 atttatatct ataacaactc ttttatattt tttactatat cttctgtagt taaaccatag
    10441 tgttttaata aatcagctgg tgtacctgat tgaccaaata catctttaac accaattctt
    10501 ctaactacta catctttagt ttctgctaaa gcttcacata cagctgaacc taaaccacct
    10561 attatactat gttcctctgc tgtaactact tttccagttt tcttagcagt ttcttttatt
    10621 aatgcttcat ctaatggttt tattgtagat atattaacaa cttctacatt tataccttct
    10681 ttagcaagtt cttctgctgc ttctaaggct tttgctacca ttattcctgt agcgattact
    10741 gttatgtcag aaccttctct taaaacagtt ccttttccta tttcaaattt ataattttca
    10801 ctgtttacat caggagttgc agctctacct aatcttatat aaacaggtcc attataatca
    10861 atagatgcaa gaatagcttg tcttgcttct aaagcatctg ctggatttaa tacaaccata
    10921 tttggtatac ctctcattag agatatatcc tctatagctt gatggctacc accatcttca
    10981 cctactgtta cacctgcatg agtagctgct atttttacat ttaaatttgg atatgctaca
    11041 gagtttctta tttgctcata gcctcttcct gctgcaaata ttgcaaaagt actcgcaaaa
    11101 ggtatttttc cacaagtagc aaggcctgct gctgtaccaa tcatgtcacc ttctgctatc
    11161 cccatatcaa aaaatctatc tggggcaact tctttaaatg atatactttt tgtagctttt
    11221 cctaaatctg catctaatac tactatatct ttattttctg caagttcttt taacacttgt
    11281 ccgtaagctt ctctagttgc tattcccatt cctatagacc tccttctaaa tctttcaatg
    11341 cttgctctgc ttgttctgca tttggagctg ttccatgcca tgcagcatta ttttccataa
    11401 atgaaacacc tttacctttt actgttttag ctattattac agttggtttt cctgtaactt
    11461 ttttagcttc atcaactgca tttcctattt cttcaaaaga atgtccattt attactatta
    11521 cattccatcc aaaagatttg aatttgtcat ctactggatt tacattcatt acttcttcat
    11581 tagaaccatc tatttgtaat ccatttaagt caacaaatgc aattaaatta tctaatttgt
    11641 aatgtgatgc tgtcattgca gcttcccata tttgaccttc ttggatttct ccatcaccta
    11701 atatagtata tactgtatag tctttgttat ctaattttcc tgcaagtgcc ataccatttg
    11761 ctgcagatag cccttgacca agagaaccag tagacatatc gattcctgct acttttttca
    11821 tgtctgggtg accttgtaac ttagaaccaa attttcttag tgttattaat tcttcttctg
    11881 ggaaaaatcc tttttctgct aatgtagcat ataaagctgg tgctgcatga ccttttgata
    11941 aaacaaatct atctctatca gccatttttg gattttctgc acatacgttc atcttatcaa
    12001 aatataatac tgtcaacata tcagctattg ataatgaacc acctggatgt ccgcttttag
    12061 cttctgcaac catttttatt atgttttttc taatattatt agaatgatct ctaagagtgc
    12121 tcatactttc ttcctcctgt tattttttaa gaccttacat ttttttatcc tacagtagat
    12181 tttacaattt tttttaaaat aatacaaatg tttttttaag attttactgc tacttttatt
    12241 tcttctccgt tcattaaagc tgtaatacct tctttaaatt cgtctaaacc aacagttcta
    12301 gtaactaaag cttctgtatt tactttccca cttgccatta aatctaatgc tgtaatccaa
    12361 gaacttggct tttgagatct acttcctata tattgtaact ctctttggat tattgcttct
    12421 tggtccattt catttaattt tttagcaaag attccaactt gtacgaaagt accttttttt
    12481 ctaagcaatc ttaaggcttc gttaactgct ggcataaatc cagaacaatc aaatcctttg
    12541 tttactccat ttccatcagt catttcattc actacatctg ccaaactttc ttccattaca
    12601 tttacagttc tatctactcc aagattttta gctatttcta acctttcctt atcttttgta
    12661 acaccagaca ttataactgt agcaccttga gatttaacaa cttgagctaa taataagcca
    12721 attggtcctg gtcctattat taaaactgta tcagaacttt ctatggtagt cttttcaaga
    12781 gctgcatgaa cacaacaagc tagtggctct gtaagggctg ctgataataa agatacattt
    12841 tcaggaagta catgtatgct ttcttctctt gatagtacat actcagcaaa acttccattt
    12901 acttgagtac caataccttt tctagttgag cataaattat aatctttagt ctcacaatat
    12961 atacatttct cacaagttgc aaaagtagtt tctgatgtaa ctctatctcc tacttttata
    13021 tttttaacat cttctcctac ttctacaaca attccagaaa attcatgacc taatgttact
    13081 ggggctttta tatttgcata ttcaccttta aatgaatgta tatctgaacc acatatacca
    13141 ctatattcta cttttatttt aaccttatct cctgtagctt ttggttcttc tacttctaaa
    13201 acttctacat tatcatagcc tggtttagtt ttaactactg ctttcattat ttttcaaccc
    13261 cttgtggtaa aattaatatt ttgttaaatt cttcttttct atctcttatc atttcaaatg
    13321 ctttgtaagt atcttcaagt gagaatctat gagataccat ttcttttaat tttatctttc
    13381 cttcattaac tagattgatt cccttagtcc attcttgtcc tggaaatggt gcagaataag
    13441 agttccaaaa accttttaat tctagttctt ttctaaatat attttcaaat gcttcttctg
    13501 ataaagttat atctgaatat gctatcccta aaaatcctat cttacttttc tttttagtta
    13561 taagtaaaca ttgttcttga gttatttttg aacctgcaca ttcaagagct atgtcaacgc
    13621 cttttccacc agtaagttct tttatttttt ctactgggtt aatatcttta gcattgatta
    13681 ttatatcagc ccctaattcc tttgattctc ttaatttatt atcagatata tcaactgcta
    13741 taattgttcc tgctcctgct attttaagtc cttgtattac taactgtccc attgttccac
    13801 tacccattac ggctacagtg tctccaactt gaacacctat attaagtact ccatgcatag
    13861 caactgctag tggctctatc attgcagcat cttcaaaatc catatctcct attggaagta
    13921 catttgtagc ttttatattt gcatactctg caaaagctcc aaaactttta gctcctaaca
    13981 ttccatggtc atcacataat gaaaataatc ctcttttaca atattcacat tcattgcaag
    14041 gtataaaagg aattgcaact actctatctc caacctttac attttttaca tccttaccaa
    14101 cttcagcaat tactccagaa aactcatgtc ctggtatagc tggaactgga tatttccaat
    14161 gttttaatac tcttggagga tctgacccac atattcctgc tgccataact tttattttta
    14221 cttcatcact ttcacatggt tttatttcta tatcttcaaa tctcatatca tttgtgccat
    14281 gtaatactgc tgctttcacg ataaatctcc tcctcttgta ttttatattt ttaaaaattt
    14341 ataaaaaata ttactctttt ttagtagctg ctaaatgtta aattttcgca tactttacta
    14401 aaaaagagta atctctatta actacatatt agaatattcc aaatgaagaa actaattgag
    14461 ataatttcaa tattccccat tttagtaagt taccacctgt atctatactt gtaaccattg
    14521 ttgttccttc tggcatcata tttgcttgtt gtaataattg tgtatgaaca tctgcaaagt
    14581 ttgtggccat ataaagtgat atagctatta ctattgttcc tgttataact gattgaacta
    14641 tattaccttt tctaaagcac actataaatg ccatataaaa tggtattgta gctaaatctc
    14701 caaatggtaa aactttgttt cctggtagta taactgctaa gaataaagtt ataggaacaa
    14761 gtataagagc tgttgatata actgctggat gtccagttgc tactgctgca tccagaccta
    14821 tataaagttc tctgtctcca tattttgctt gtaagaaatc tcttattgat tctgaaattg
    14881 gaattaaacc ttccattaat attctaacca ttctaggcat aagtaacatt actgcaccca
    14941 ttgatatacc tacattgaat attcctgcta catcatatcc agcaagtgct ccaagtatta
    15001 tacctagtat aatccccatc atcattggtt ctccaaatat accaaatctt ttttgtatag
    15061 agtctgggtc tgcttgtatt ttatttattc ctggtatttt ttcaacaaat ttacctacta
    15121 aatatccaag tggagcaaat gaagctgttg aaccagttgg taatgctata ccttctaaat
    15181 cataaaactc ctgaacattt tttgctgtct tttctgcaat aaatagtact aataattcca
    15241 ttaatatcgc acagaatata gcccaaacat aacttcctgt tactatatat ccaactgcac
    15301 ctgctactat aaagtgccaa taattccata tatcaacgtt taatatcttt gttttattga
    15361 agtataataa tagtaagttt actaataaac aaactggtat taatattggt gcaactggtg
    15421 aagcccatgt tgctgatgct gttgttggcc aaccaacatc tattactgtt aaattaagtc
    15481 cgaatctttc aaccattcct tgagccgctg gccctagtgt tgatgttaat aggtttacaa
    15541 ctaagtttat acccacaaat cctataccaa ttgtaagacc tgactttaaa gcttttgtaa
    15601 acttaattct aaatatcatt cctagtacta caattgttat tggtagtatt actgtcggtc
    15661 ccatatttaa aatgtattgt actactgcta ataatttatc catttataaa tcctcctttt
    15721 tctgttttat aaaatattat ttcaatgtat ctatgatagg attattttaa agcatctaat
    15781 atttttttat ctaatgctgc tgtatttact ccagttatat atgctgttgc ttttattgct
    15841 ggtatattat aagttgttgg tagtatagtt gttgatacta ttaaatctgc tccttcttgc
    15901 tttgttgcac attcagctat tttgcattgt ataacttctg cttgtatatt attttcctta
    15961 actaatctct ctaccttgtc acaaactact gttgatgttg ctattcctgc tccacatgct
    16021 actaatattt ttttcatttt tatttcctcc taataatttt tatttttata ttttttattt
    16081 ttagtacttt tttattttta taaagacttt agtgtcttta aaacttcttc tttatcagta
    16141 gaactcatca acttatcaac taactcttta ttttgcataa ttcccattaa ttctgttaat
    16201 acagataatt gactgtgtgg ttgattaagt cctaatacga aagccaaatt tacaggtact
    16261 ttttcatctt ggtcttccat agaactaaat actactggtt cattaaaagt acagactgct
    16321 ataaattgct ctttaatata ttctgcatct gtatgtggta tggctactcc ataatcgcct
    16381 agatttaaac ctgttggaaa tacattttct cttgctaata ttttttctcc aaattcttct
    16441 tttacaaagc ctaaatcaaa aactttatta tgtatttctt taaagaaatc ttctttcgtt
    16501 ttacaatcca ttttaaggtc tataacttta tcacttacaa aatttataaa ctccatattt
    16561 acacctccta attgcctttg tcttatatct tagtttttct tgactttata taaagcatgt
    16621 tgtgtgccaa aaagtggctt tttttgttaa gcaaaagaaa atgttttccc aaaaataatg
    16681 ctctcttcta ggattttagc tgactttcat tgataaattt tttttgattt ttattttaat
    16741 ttttacttgt taatcactta actagccata cttttttata ttttttgtgg ttttttttgt
    16801 tagagaaatg atttttttgt tttttacaaa
//
LOCUS       DAEPXF010000079        16825 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain HB27
            SAMN13376389-rid12479423.denovo.083, whole genome shotgun sequence.
ACCESSION   DAEPXF010000079 DAEPXF010000000
VERSION     DAEPXF010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13376389
            Sequence Read Archive: SRR10518432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 16825)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16825)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001069624.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001069624.1
            Genome Coverage       :: 315x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/15/2021 01:54:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,165
            CDSs (total)                      :: 4,076
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 89
            rRNAs                             :: 10, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 10 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 19 of 69
            Pseudo Genes (incomplete)         :: 44 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 10 of 69
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16825
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13376389-rid12479423.denovo.083"
                     /strain="HB27"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="China: Hebei"
                     /collection_date="2017"
     gene            186..407
                     /locus_tag="KRL67_002215"
     CDS             186..407
                     /locus_tag="KRL67_002215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore coat associated protein CotJA"
                     /protein_id="HBH1523089.1"
                     /translation="MGYLKEDKQDYCCEFARPYINRQIYDDTYNPKTALKRGTLFPEL
                     DLIESNNYNDWLYANPKNRVRAKKGGNMY"
     gene            407..670
                     /locus_tag="KRL67_002216"
     CDS             407..670
                     /locus_tag="KRL67_002216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore coat protein CotJB"
                     /protein_id="HBH1523090.1"
                     /translation="MSLNRRELLERISEYQFACIELNLYLDNNPRDKKALDSYNRYCD
                     KFTQAVCDYESRYGALTNFGYESSEYPWSWISEPWPWDKSFYK"
     gene            732..1304
                     /locus_tag="KRL67_002217"
     CDS             732..1304
                     /locus_tag="KRL67_002217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese catalase family protein"
                     /protein_id="HBH1523091.1"
                     /translation="MWIYQKTLEHPVNIRQADPRMAKYIMTQLGGPNGELAAATRYLQ
                     QRYTMPTGKSRALLTDIGTEEMAHVEIISSVLYQLIGSCTPEELKAAGLGSNYANFGH
                     GLQPVDSNGVNFTTSYINVFGDSVTDLHEDMAAEQKALATYYQLINLTDDPDLKDILR
                     FLGEREVVHYQRFGEALMDVYEFTECKHQF"
     gene            complement(1851..2171)
                     /locus_tag="KRL67_002218"
     CDS             complement(1851..2171)
                     /locus_tag="KRL67_002218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBH1523092.1"
                     /translation="MKNTIVLHYIEKYYCENLILKYLSNSANISKSECSRCFKLSLNI
                     TPYKYFTEFRLLKATQFLKGTNEIISDIAIKVVFHQIIYFSKCFKEKMGYSPKVYRNI
                     KKEI"
     gene            2810..3343
                     /locus_tag="KRL67_002219"
     CDS             2810..3343
                     /locus_tag="KRL67_002219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HBH1523093.1"
                     /translation="MQYISYYHSPIGNILLASDNIGLTGLWFENQKYYAYNLDQEHQE
                     KDLPIFEHTKKWLDIYFSGREPNFTPSLHMVGTPFQTKVWKILQQIPYGKTITYGEIA
                     YKVAQQKGISKMSAQAVGGAVGHNGISIIVPCHRVVGTNGSLTGYAGGIDRKIKLLSL
                     EKVSVQHYFIPKKGSAL"
     gene            complement(4262..4963)
                     /locus_tag="KRL67_002220"
     CDS             complement(4262..4963)
                     /locus_tag="KRL67_002220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid stabilization protein"
                     /protein_id="HBH1523094.1"
                     /translation="MDEKILELLQEMQGNISNMQGSISGIEGTVNGMQGNINYMQGTI
                     SDMQGSINGIEGTISDMQGSINGIEGTISDMQGNINGIEGTISDMQSNINGIEGTISD
                     MQSNINGIESTISDMQSNINGIEGTISGMQGTISDMQGNIKEVNNRLDNMEPDIKDLK
                     TGQVETSSRLDTIQSDVTEIKSDVKINRNTLDTVIKHTSSLVEDTSDIKNDLNNIEIV
                     TSKNWNDIAKLKSVK"
     gene            5429..5842
                     /locus_tag="KRL67_002221"
     CDS             5429..5842
                     /locus_tag="KRL67_002221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003507530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3795 domain-containing protein"
                     /protein_id="HBH1523095.1"
                     /translation="MKMPKESIDMIMFAPCGMNCMVCYKHCYHKKPCAGCLNSDTGKP
                     EHCRKCKIKDCVRQKGLFYCFECFDFPCKLIKNLEKSYNKRYQASLMENSKFVQQHGL
                     EKFMQKQKERYTCSKCGGIISIHDRECSECQEKMK"
     gene            5917..6453
                     /locus_tag="KRL67_002222"
     CDS             5917..6453
                     /locus_tag="KRL67_002222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GrpB family protein"
                     /protein_id="HBH1523096.1"
                     /translation="MIGLKCGMVKLLAHQMIWDKSAKDVITLLKSIWDKTAIDIQHIG
                     STSIPSISAKPIIDIVVGVASLEEAKLYIERLEQCGIFFRGQDVPKQLLFVMGDFEKN
                     TRTHHIHVVEWNSVAWNNYINFRDYLNTFTDKAKLYDSHKQKLALQFEDNRKIYTQRK
                     QELINILLEEARLWRINT"
     gene            6613..8187
                     /locus_tag="KRL67_002223"
     CDS             6613..8187
                     /locus_tag="KRL67_002223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClC family H(+)/Cl(-) exchange transporter"
                     /protein_id="HBH1523097.1"
                     /translation="MKKDTSHIIKRAEHFQVILIGEGLMVGAVAGLIVLLYRILLGQA
                     GTWLSQILKFVEGSTIKIIAWFAVLAVLAWIVSKLVDWEPMISGSGIPQLEGEMTGKI
                     KQVWWRVLPTKFIGGFLSLMGGLALGREGPSIQLGAMAGKGISRALDRGKTEEKFLLT
                     CGASAGLSAAFHAPLAGVMFSLEEVHKNFSVSVLISVMTASLTADYISANFLGMESVF
                     QFDIGNVLPQDYYGLIVILGVILGVMGAFYNWFTLYVQSLYKKIPKIGTFGKLLIPFI
                     MAGILGLVMPDVLGSGHNLVEELTSHEYIFGMVVLIFVIRFIFSAVSFGSGAPGGIFF
                     PLLVLGAFIGGIFGMVGVKVFGMNPDYVNNFVLLAMAGYFTAIVRAPLTGIILIFEMT
                     GSVSQMLSLSVISIVAYIVATLMKSKPIYESLLERLLEKNGEPVHKERGEKILDQFSV
                     MFGSPVANKEIQDVQWPENCLLVAIQRGGEEIIPRGKTILRPSDVIVTMTDERDSGAV
                     YDKMENLCKEHTSHIF"
     gene            complement(8859..9464)
                     /locus_tag="KRL67_002224"
     CDS             complement(8859..9464)
                     /locus_tag="KRL67_002224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urea carboxylase-associated family protein"
                     /protein_id="HBH1523098.1"
                     /translation="MINWNLWRTLNMEYIINPCSGIKIKVTKGQKIEIVDIEGGQVVD
                     FFAEIQDDSTEFISPGVTIDCNESLRLQVGNLMYSNKYKPMFKILYDDVREHDLLHPC
                     CRPEMYDFFYHNGENHPNCFDNINHALGTSHQIIHPINLFMYTKLNTDGSIEVCEPLS
                     KANDKIVLEALENMTLGIAACSVSESSCNSRKCTSIKIVVD"
     gene            9834..10403
                     /locus_tag="KRL67_002225"
     CDS             9834..10403
                     /locus_tag="KRL67_002225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GrpB family protein"
                     /protein_id="HBH1523099.1"
                     /translation="MKKKLEDMTLEELWNLFPIFLTNHKECWKNWFKEEQYRLTEILP
                     MQKIVRISHIGSTSVSTIYAKPIVDILIEIIPDYNMKDIKDLIESNGYICMSKSSSRL
                     SLNKGYTVDGFEERVFHLHIRYVGDNDELFFRDYLIDHPSVAKEYEKLKLDLWKKYEH
                     NRDAYTDSKLDFVREFTEKAKVLYSNRYN"
     gene            complement(10740..12077)
                     /locus_tag="KRL67_002226"
     CDS             complement(10740..12077)
                     /locus_tag="KRL67_002226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HBH1523100.1"
                     /translation="MDTYSLISTSINFILILINIFIAFFLYKLTSYFIDFRNHWIFKI
                     ILILGYSIISQMIIWLSDPVNILFTLFGYFILLIICSKSKLIPIISVIMILYPIVIGL
                     NFLFINNPLYGELIHSKEYILILSIVQIVSALIKLLFWFLIHIFLKNRLVNIKQYLSN
                     KIWIFIDIVCISSFLSILIAIILPPTNLIPMGSNKNIILGNLPYGTYLIILSAILANI
                     GVILLLQPLIENVKMKIEKQANHIKEKYYNLLENQQIQIRKMRHDMNNHFQMIESYLE
                     VEDIDGAKKYFNQLKIGIGTLSGKQFCKNQALNSILNLRHTSLEENGVDVHFNIDIDN
                     HLGIEPIDLCTIFSNSLDNAIEASLKIPDFSNRKVVLKARCEKEYFSYLLTNNKVNPI
                     NRKQELFISDKKDSNSHGYGIENIREIVNKYNGKLDISYTESEFSLFLYIRLF"
     gene            complement(12141..12851)
                     /locus_tag="KRL67_002227"
     CDS             complement(12141..12851)
                     /locus_tag="KRL67_002227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBH1523101.1"
                     /translation="MTSICICDDEKQQRNMLNKIITREMDLWGYPYQIKEYSNGESLV
                     YNLEHENYYIDIIFLDIELGKSNGVDIAKTIRKHSKDTIIIFVTGFSDYVFHGYDVGA
                     LNYILKPYKTEKICKVLKEALKKLEQWKENFITVQIGTNLCKINTKDILYFKSELRKL
                     TVVTFDKTLEYYGKLNDMETVLPSNFIRTHQRYIVNLSHVDSVTQTYAKIQNEKIPIS
                     RKKYQEVVGKFTRYMLES"
     gene            complement(13287..15398)
                     /locus_tag="KRL67_002228"
     CDS             complement(13287..15398)
                     /locus_tag="KRL67_002228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBH1523102.1"
                     /translation="MTINKLYSKLRAYNWKNYTTLFFCIILSVVLVTSYALIYFSPTI
                     QNILPTGGDSRKQATMIFSVAILGCAVFTTYASSLFLKFKSKEIGIFMALGTDKKKLK
                     KLLFSEILLISIICCFLGLILSIPVSFGIWKIFQLVIIDTREMTYHFGTQGFIYGFIF
                     SIFVTLCIFILSVKFINRTNIIDILNDQRISEPIQDVKDWYGILGTIFIILGIFLGYL
                     VPSLTINKLNYRLPSIWSFTYLLSLIGIYMIMTYIVVHAKRGKHPEKYYKNIISTSMM
                     RFMGRQTVKNMCVISFLIAGALFAVFYVPNMVTGIYDTLKNNPIDYTFTYNQRDKQVS
                     KKDIEQLADKYNIKIQNYMEIPSIELIVDGVESIYHKDGTIEKVYMKKSSYDMFFNSK
                     DFSKLIGKPITIKNGEYLAVVNKGNENQDYKYTKFISSPVTEKEIPFRYAGKLIFNGS
                     FFQNRSMNAYVLNDEDYKSFTKDLSIKNYNNSILFNVDKDTYAFANDLKKEIIKRTSK
                     EMGMFSGYDEYVKKRYESTGRKYFMDEEYPSGEGHLKLDPDNNQLYLNWKYYPNFKIL
                     QSQDMIKNMAVFLMLFIYVAIICFTAVGIIAYTRGVTIAINHKQVFMDLNRLGANNDY
                     IKFCIRNQLKKVFSYPLIAGSFLIYLFNFMIMLNNDGRIVNSEVNALLINLGFIGICG
                     VYMYLIYLLTYKKFKKIIGIL"
     gene            complement(15388..16140)
                     /locus_tag="KRL67_002229"
     CDS             complement(15388..16140)
                     /locus_tag="KRL67_002229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBH1523103.1"
                     /translation="MLKVNQLCKSYKTGNKTYPILKNISFEINKGEFVAIMGSSGSGK
                     TTLLNCISCFIPIDNGEIILGNNNIRNLKEKELSEVRNQQLGFVFQDFMLFDGLSVFE
                     NICVPQIIKGGQVLQMENRAVNLCELFGIEHIRKKYPAEISGGEKQRTAVARALMNNP
                     DLILADEPTGNLDSKSCRTVIESFLKAKQELDATILMVTHDSFAASFCDRVIVLKDGI
                     IYKEVVKSNSRHKFLDELLEVLKEMGGGQDDN"
BASE COUNT         5962 a         2079 c         2657 g         6127 t
ORIGIN      
        1 atagatattt atgaaagatt aattagggaa atttttagag gtaaaataag aacaaaaaaa
       61 gatacttatt taattattca atcttattaa agacctatta aaactactga ttataatata
      121 gaaccaatat acaattttta aaaataatgc tattttaaaa ttctaaaaat taaggaggat
      181 aattaatggg atatttaaaa gaggataaac aagactattg ttgtgaattt gccagaccat
      241 acatcaatcg tcaaatttat gatgacacat acaatccaaa aacagcatta aaaagaggta
      301 ctttatttcc ggaattagat ttaatagagt caaataacta taatgattgg ctatatgcaa
      361 atcctaaaaa tagagttaga gctaaaaaag ggggtaacat gtactaatgt cattaaatag
      421 aagagaactt ttagagagaa tttcagaata tcaatttgcc tgtatagaat taaatttata
      481 tttagataac aatccaaggg ataagaaagc tcttgattca tacaacagat actgcgataa
      541 atttacacaa gctgtgtgtg attatgaatc tagatatgga gcattaacta attttggata
      601 tgaatcaagt gaatatcctt ggtcatggat atctgaacct tggccatggg acaaatcatt
      661 ttataaataa aattattaag ctttaaaatt agatttatta ttaaatatat attaaggagg
      721 agtaagataa tatgtggatt tatcaaaaaa cactggaaca tccagttaac ataagacaag
      781 cagaccctag aatggcaaaa tatatcatga ctcagttggg aggacctaat ggtgagttag
      841 cagctgcaac tagatatctt caacaaagat atactatgcc aactggaaaa tctcgcgcac
      901 ttttaactga tataggtaca gaggaaatgg ctcatgttga gataatttct tcagtgttat
      961 atcaattaat aggcagttgt actccagaag agcttaaggc tgctggtctt ggtagtaatt
     1021 atgctaattt tggacatggt cttcagccag tagattctaa tggagtaaac tttactacaa
     1081 gttatattaa tgtctttggc gattcggtaa ctgatttaca tgaggatatg gctgctgaac
     1141 aaaaagcatt ggctacttac tatcaattaa taaatttaac tgatgaccct gatttaaaag
     1201 atatattgag atttttgggt gaaagggaag tagttcacta tcaaagattt ggtgaagcac
     1261 taatggatgt ttatgagttt acagagtgca agcatcagtt ttaatgaagt attaaaagtg
     1321 aatggagagt aagcatagtt ctaatgaaga attaaaagtg aaaagagaat aaacataagt
     1381 tctaatgaag aattaaaagt gaatggagaa taagcatagt tctaatgaag aattaaaagt
     1441 gaaaagagaa taggcataag ttctaatgaa gaattgaaag tgaacagaga gtaagcatca
     1501 tttttaatga agaattaaaa gtgaaaagag aataagcata agttctaatg aagtattaaa
     1561 aatgaaagga gaataagcat catttttaat gaagtatcga aaataaaaag agtgtaaata
     1621 ttaactctga taaactatca aaaataagag gtaaaggttt tagttgattt cttttatctc
     1681 ttatttttta ttgattaaac agattacttt gatatttaaa tatattattt tagcaaaaaa
     1741 agtccaatta aaagttcaaa gtattaccat agttcaaagc attgccgttg ttggcaagtt
     1801 tttgacaata ctttttattt actctttgaa taatcaaaag aaataaaaaa tcatatctct
     1861 ttttttatat ttctgtacac ttttggagaa tatcccattt tttctttaaa acatttacta
     1921 aaatagatta tttgatgaaa aacaactttt atagcaatgt cacttattat ctcatttgta
     1981 ccttttagaa attgagttgc ttttaataag cgaaactcag taaaatactt atagggtgta
     2041 atatttagac ttagtttaaa gcatctggag cactctgatt tactgatatt agcactgttt
     2101 gataagtatt tcaaaattaa gttttcacaa tagtattttt caatataatg taaaacaata
     2161 gtatttttca atataacgta aaattttttg catatggata ttaattctat ttttgtgttt
     2221 tattggtgac aaaacaatat tttttatcat aataagccaa agaaaagaaa tagaaactaa
     2281 tgcttcatac atatacagtt catttttact tttttctaac cttattaatc gttgtagaat
     2341 agaaaatact tcattgcgcc aattatattt atgtgtaaaa tgataaatag caaattgttc
     2401 gttttctgtt attctattta ctaacgattt tactggattt ctaacataaa aatacttttc
     2461 ctttttacaa cctattctta cttttcccat ttgaaagtat tatgatatta cttgatgtta
     2521 tattgatatt tttttgacct atttagtgat aaaattataa tatacacttc tctaaaagtt
     2581 aatacagtag gaagcttaaa tgtatgaatt ttatatccac caaagggaaa gggagaaaaa
     2641 aaggatgaac agatacaggt aaagataaaa ctagtatgat atgaggatag atattataac
     2701 aaaagtgtag tcagaaggaa attatattag tatgctttta cataatggca gatagaaaag
     2761 aagtgtctaa cgatttgagt gaagttttca gaatataagg aggaattgta tgcaatatat
     2821 tagctattac catagtccaa ttggaaatat tcttttagca tcagataata ttgggttaac
     2881 tggtctttgg tttgagaatc aaaagtatta tgcttataat cttgaccaag aacatcaaga
     2941 aaaagacttg ccaatttttg agcatacgaa gaaatggtta gatatttatt tttcgggaag
     3001 agagcccaat tttacaccat cactccatat ggtaggtact ccttttcaga ctaaagtgtg
     3061 gaaaatttta caacaaatac catatggtaa aacaatcact tatggagaaa ttgcatataa
     3121 agttgctcaa caaaaaggaa tatctaaaat gtcagcacaa gcagtcggtg gtgcagtagg
     3181 gcataatgga atctctatta ttgttccatg tcatcgtgtt gtaggaacaa atgggagttt
     3241 gacagggtat gctggtggaa ttgatagaaa aataaaattg ttatctttag aaaaagtatc
     3301 tgtgcaacat tattttatcc caaagaaagg gagtgcatta taaagccatt aaatttagaa
     3361 attcttaatc atttatttga tatacaaaca tctaattttg aaagtaataa cttgcaattt
     3421 taaaaaacta ttctttaaac tatatgttat acaaccttat tcctagtaaa cattatacaa
     3481 ttttaaaggt agtttttaga gcttgtattt tggaagttta ttttcttttt tcatgtgagt
     3541 agttatatgt ttagatgcag taatttatat gctacaaaca gggaaataag gagcagacaa
     3601 aaaatactta gataaaatgt tcaaaatata ttttgtatat tttggaaata gtaagagtgt
     3661 taaatataaa tggaaaattt ataatgattg atattgaagc tactcattaa gtattaagaa
     3721 ccatacaaga tgaggttgaa atttgtatgc ttcgttttat gtaaaaaatc taagtgagtt
     3781 agaaatgaaa gaattattta taaaaaatag attaagtatt gggaaaatat gaaaaataga
     3841 tgttgaataa aacttagttg attggttaat ttaaccaaac acttaaagta ttaaaagaaa
     3901 aaattattat tcttaagcaa gaaggtataa gtggaaaaat gaaaacaaga ttttatcctt
     3961 ttataagtga taaggggata tgttttaatc aaggatggac ttttatttta ggtcaaaaaa
     4021 ttatatagct atattataaa atataaggga gatacacaac tttttagtgt tcaatctccc
     4081 ttacttttta ttattatatc aaggctcatt tagccatgtt aattttagta taaaatgagt
     4141 atttttctaa taaattattt ataagtaaag tgtgttgtcg gataatcagt atttctgtat
     4201 aaaatttggt ataactttaa agaagtatgt gaaattttca ggtatttttt actatggtat
     4261 attatttaac tgattttaat ttagcaatat cattccaatt cttagatgtg actatctcta
     4321 tattgtttaa atcattttta atatctgatg tatcttctac taatgaagag gtatgtttaa
     4381 tcactgtatc taatgtattt ctgttaattt ttacatctga cttaatttct gtaacatcac
     4441 tctgaatagt atcaagtcta ctactagttt caacttgtcc agtttttaag tctttaatgt
     4501 caggttccat attgtctaac ctattattga cttctttaat attaccttgc atatcgctaa
     4561 tagtgccttg catgccacta atagtgcctt cgataccatt gatattgctt tgcatatcgc
     4621 taatagtgct ttcgatacca ttgatattac tttgcatatc gctaatagtg ccttcgatac
     4681 cattgatatt gctttgcata tcgctaatag tgccttcgat accattaata ttgccttgca
     4741 tatcgctaat agtaccttca ataccattga tactgccttg catatcgcta atagtacctt
     4801 caataccatt gatactgcct tgcatatcgc taatagtacc ttgcatatag ttaatattac
     4861 cctgcatgcc gttaacagtg ccttcgatac cactgatact accttgcata ttactaatat
     4921 taccttgcat ttcctgtaat aactctaata ttttttcatc catcactatc acactcctgt
     4981 tttattatta taacataatt taggtgttat attccacaaa cgctatgtca tatttaattt
     5041 ttaatcatat aattatctgc tattcaaaac atggttaaat atatctatat tgagaacttt
     5101 tttattaatg aatcttgtgt attgtctaag tgtctaagat actataataa gtgtttttga
     5161 ggcatgtgtg gttatatttg tatttaaaac tgctggtaat atcatatact tttaaatatc
     5221 ccttaagtta tttatattga ttacttgtgt ttgaatattt tgattagtag cttacttttt
     5281 atcttttttt attcttagtt ttaaactttc atatttcctt gaaaatttat tggcaatgaa
     5341 agtgcatgaa ttttttagct attttttatg ttacaatatg aatatataac aattcgacaa
     5401 attggatttt tgaaaggaat aaaatgttat gaaaatgcca aaagagagta ttgatatgat
     5461 tatgtttgct ccttgtggaa tgaactgtat ggtttgctat aagcattgtt atcacaaaaa
     5521 gccatgtgct ggttgtttga acagcgatac aggaaagcca gaacactgcc gtaagtgtaa
     5581 aataaaggat tgcgtccgtc aaaaagggtt gttttattgt tttgaatgtt tcgattttcc
     5641 ttgtaaactc ataaaaaatc ttgaaaaaag ttataacaag agatatcaag caagccttat
     5701 ggaaaatagt aaatttgttc agcagcacgg tttggagaag tttatgcaaa aacagaagga
     5761 gagatatact tgttctaaat gtggaggaat tatatctatt catgataggg aatgtagcga
     5821 gtgtcaagaa aaaatgaaat agctagatca ctgtttatca agatggaaaa aattgtaatg
     5881 tgtatataaa ggtggattat attggggtgg tatatagtga taggtttaaa atgtggtatg
     5941 gttaaattat tggcacatca gatgatatgg gataaaagtg caaaagatgt cattacatta
     6001 ttgaaaagta tatgggataa gactgccatt gatattcaac atattggaag cacttctatt
     6061 ccaagtatta gtgcaaagcc tattattgat attgttgtag gtgttgcttc tctggaggaa
     6121 gctaaattat acattgaaag gttagagcaa tgtggaatat tttttcgagg tcaggatgta
     6181 ccaaaacaat tactttttgt tatgggagat tttgaaaaaa atactcgtac tcaccatatt
     6241 catgttgttg aatggaatag tgttgcatgg aataattata tcaattttcg agattatcta
     6301 aatactttta cagacaaagc aaaactctat gatagtcata aacaaaaatt agcattacag
     6361 tttgaggaca atcgtaaaat ctatacacag agaaagcaag aactaataaa tattttgctg
     6421 gaagaagcac gactgtggag aataaatacc taaaataata aattcacatt tattaagaaa
     6481 ctgaacaatc attttcttat atttagtaaa taaaaatcta tttttgttga ttaaaaaata
     6541 tctgtacaca ctcatgactt tagtcattgg aggttcagat tgcgtagaat tattggatga
     6601 aggaggaaca gtatgaaaaa ggatacatct catattatca aacgggcaga gcactttcag
     6661 gtaattttga ttggagaagg acttatggtt ggtgctgtag ctggtttgat tgtgctttta
     6721 tataggatac ttttagggca ggctggtact tggcttagcc aaattttaaa atttgtagaa
     6781 ggttctacaa taaagataat agcttggttt gcagtacttg cagtgcttgc gtggattgta
     6841 tcaaagttgg tggactggga gccaatgatt tctggaagtg gtatccctca gcttgagggg
     6901 gaaatgacag ggaaaataaa acaagtatgg tggcgagtat tgccaacaaa atttattgga
     6961 ggattcttat cacttatggg tggtcttgct ttgggaaggg aaggtccttc aattcagctt
     7021 ggtgctatgg cagggaaagg tatttctaga gctcttgacc gagggaagac agaggaaaaa
     7081 tttctgttaa cctgtggagc aagtgctgga ttatcagcag cctttcatgc accattggcg
     7141 ggtgttatgt tttcacttga agaagtccat aaaaattttt cagtatctgt tttaatttct
     7201 gtgatgacag catctttgac agcagactat atttcagcca atttcctagg aatggagtct
     7261 gtattccagt ttgatattgg aaatgtgttg ccacaagatt attatggatt aatagtgatt
     7321 ttaggtgtta ttcttggagt tatgggggca ttttacaatt ggtttactct atatgtgcag
     7381 tctttataca agaagattcc taaaattggt acgtttggaa aactgttgat tccatttata
     7441 atggctggta ttcttgggct tgttatgcct gatgttctag ggagtggaca caatttagtt
     7501 gaagaactta caagtcatga atatattttt ggaatggttg ttttgatttt tgttatacgt
     7561 tttatatttt cggctgttag ttttgggtca ggtgcaccag ggggaatatt ttttccattg
     7621 cttgttttag gagcatttat aggtggaata tttggtatgg taggtgttaa agtttttgga
     7681 atgaatcccg attatgtaaa taactttgtg cttttggcta tggcaggata ttttacagct
     7741 attgtgcgtg ctcccttgac tggtatcata ttaatttttg aaatgacagg ttctgttagt
     7801 caaatgcttt cactatcagt aatttcaatt gtggcatata ttgtggctac actcatgaag
     7861 tctaaaccta tttatgaaag tcttcttgag aggttacttg aaaaaaatgg agaacctgta
     7921 cataaagaga ggggcgaaaa gatactagac cagttttctg ttatgtttgg gtctcctgtt
     7981 gcgaacaagg aaattcagga tgtacagtgg cctgaaaatt gtcttttggt agcaattcaa
     8041 agaggtggag aagaaattat tcctagaggt aagacaattc ttagaccaag tgatgtaatt
     8101 gtgactatga cagacgagag agatagtggt gctgtgtatg ataagatgga aaatttgtgt
     8161 aaagaacata cgtcacatat tttttaggga taatgatgaa tgtttaataa tgatagaata
     8221 tttaatgaag atttgattaa aaaattggta atttgaaaga gcgttaaagg attaatataa
     8281 gaaaatgaaa atgtgatatc ttagatgtaa aagagtttga agaaaataat tgtatatatt
     8341 tgttaatgag attatatgga ctaattgtat atttatacgg tactaaaagt tgcactattg
     8401 tatgaggaat gggaataaag ggtttgaaaa aataagtaaa agacttttta gatattctta
     8461 ttgtgatatt ttaattgcga tattttggga agaatagaat ttatttataa atgaaaataa
     8521 ttaaaagggg ttaaggttag atgaatatcg ataaggaggt attattagat aattaactta
     8581 acatctaaca gatagagttt ttatgaaaac agtgtgtatt gcaataaaaa ggtacacgct
     8641 gttttttcgt tctgttacgc aatagaatgc tattttttag agtacgagta taaaacccat
     8701 attcccttgt aattgtgtct gaaaaacatc ataaaatcaa gggattttta cacctaaatt
     8761 cgtaacatac cataattaat tgaggacgca agtgcttatt cttttgcgcc attgatggta
     8821 ttttctgatt tttacattta tttgataaat gaaatttgtc aatcaaccac aatcttaata
     8881 gaagtacatt ttctgctatt gcaagaactt tctgaaacac tgcacgcagc aattcctaaa
     8941 gtcatgttct ccaatgcttc aagaacaatt ttatcattgg cttttgataa aggttcacat
     9001 acttcaatac ttccatctgt attgagtttt gtatacataa ataaattgat ggggtgaatg
     9061 atttgatgtg aagttcccag tgcgtgatta atattatcaa aacagtttgg atgattttct
     9121 ccattgtgat aaaaaaaatc gtacatctca ggtctacaac atggatgcaa taagtcatgt
     9181 tcccttacat catcatataa aattttaaac ataggtttat acttattaga atacattaaa
     9241 tttccaactt gtaatcttaa tgactcatta caatcaatcg taacccctgg agatataaat
     9301 tcagttgaat catcttgtat ttctgcaaaa aaatcaacga cctgtcctcc ttcaatatcc
     9361 acaatttcaa ttttttgccc tttagttact ttaattttta taccactaca aggattgatt
     9421 atatattcca tatttaatgt cctccataaa ttccaattta tcattctgtt ctatgttctc
     9481 attttaacac atactctgtc tcttttctat ttgtttcttg acgtttataa catagtctat
     9541 cattttttaa ttttcataaa ggtgttgtag tcttttttca agattacagc atttttttgc
     9601 ttaaatttgg cattttcgca atattactgt atttagaagt aagaaaaaat attctatatt
     9661 taaatagata caaaagtgta agaaaggtga aaatctgtta taatacatta aaaactaaat
     9721 ttagaaaaat ttatataatt agaaaaatga tttgataaaa ataaagttaa atgttaacta
     9781 aataaatttg aaaaagaaaa aatataaagt acaaatttaa aaggagataa ctaatgaaaa
     9841 agaaattgga agatatgact ttagaggaat tatggaactt gtttcctata tttttgacta
     9901 atcataaaga atgttggaaa aattggttta aagaagaaca atatagacta accgaaatat
     9961 tgccaatgca aaaaattgtt agaataagtc atattggaag tacttctgtg agtacgattt
    10021 atgccaagcc cattgttgac atactgattg agataattcc tgattataat atgaaagata
    10081 ttaaagattt gattgagtcg aatggatata tttgtatgtc taagagcagt agtaggcttt
    10141 cactcaataa agggtataca gtagatggat ttgaagaacg agtttttcat ttgcatataa
    10201 ggtatgtagg tgataatgat gagttgtttt ttagagatta cttaattgac cacccaagtg
    10261 ttgcaaagga gtatgaaaaa cttaagttgg acctttggaa aaagtatgag cataatcgtg
    10321 atgcttatac agatagtaaa ctagattttg ttagagagtt tactgaaaag gcaaaggtat
    10381 tgtattctaa tagatataat tagaaatagg tacaattaga aatttatctt atatatcgta
    10441 ttatattttt taagaaagta attaatagaa aaaggggttg tttcaaaatg gagagataaa
    10501 tccctttttc attgtttaaa tgataaaaat atatttgatt tcatataaaa aagagtgcct
    10561 catcaactaa aaagtttatt ttgagacaca catattttta ctaaggtaaa taaaaatatc
    10621 acatttgatt aaattttaag ttaatgaata tatgtgcaaa tatttaatta taagttagta
    10681 caattttaca tagtataaaa caatataatt tgtattatat aagataatct aattttagac
    10741 taaaataatc taatataaag aaataaagaa aattcactct cagtataaga gatatccaat
    10801 tttccattgt acttattaac aatttctcta atattctcaa taccatatcc atgagaatta
    10861 ctatcttttt tatctgaaat aaatagttcc tgttttctat tgataggatt taccttgttg
    10921 tttgtcaata aatagctaaa atactccttc tcacaacgtg cctttaatac cacttttcta
    10981 tttgaaaaat caggaatttt caaagatgct tctatagcat tatctaaaga atttgaaaaa
    11041 atagtacata aatctatagg ctctataccc aaatgattgt ctatatctat attaaagtgc
    11101 acatcaacac cattttcctc caaacttgta tgacgaagat ttaaaattga gttaagagct
    11161 tggtttttgc aaaattgttt gccactaaga gtacctatac ctatttttaa ctgattaaaa
    11221 tactttttag ctccatcaat atcttcaact tctaaatacg attcaatcat ttgaaagtga
    11281 ttattcatat catgtcgcat tttacgtatt tgaatttgtt gattttctag taagttatag
    11341 tatttttctt ttatgtgatt tgcttgtttt tctatcttca tctttacatt ttcaatcaat
    11401 ggttgtaaaa gaagtatcac accgatattt gccagaattg ctgacaagat gattagataa
    11461 gtgccataag gtaagttgcc taagataata ttcttattac tacccatagg gattaaatta
    11521 gtaggtggca gaataatagc aattaatatt gataaaaatg aagatataca tacaatatca
    11581 ataaaaatcc atattttatt tgataagtat tgttttatat tgaccaatct attctttagg
    11641 aaaatatgta tcaaaaacca aaataataat ttaatcagag cagaaactat ctggactatt
    11701 gaaagtatta aaatatactc ttttgagtgt ataagctcac cgtatagagg attgtttata
    11761 aaaagaaaat taagtccaat tacaatagga taaagtatca tgataactga tattatagga
    11821 attaatttgc ttttgctaca gattataagt agtataaaat atccaaatag tgtaaataaa
    11881 atatttacag ggtcggaaag ccatattatc atttgactaa taatagagta gcctaaaatc
    11941 aatatgattt tgaaaatcca atgattccta aagtctataa aataggaggt caacttataa
    12001 agaaaaaagg ctatgaaaat attgattaat attagaataa aatttatgct tgtagagatt
    12061 aaagaataag tgtccattta atataccttt ctattaatta agattctaga aactaagttt
    12121 taaaaattaa atcttaaaaa ttaagattct aacatatacc ttgtaaattt tcctacaacc
    12181 tcttgatatt ttttacggct tataggaatt ttttcattct gaattttggc gtaagtttga
    12241 gttacactat ctacatggct tagattgaca atataccttt gatgtgtacg tataaaatta
    12301 gagggaagaa cagtttccat atcatttaat tttccatagt attctaatgt tttatcaaag
    12361 gtaactacag ttaattttct tagctcactt ttaaagtaga ggatatcttt tgtattaatt
    12421 ttacaaagat tagttccaat ctgaactgta ataaagtttt ctttccattg ttctaatttt
    12481 tttagtgctt cttttaatac tttacatatt ttttcagttt tataaggttt taagatgtag
    12541 ttcaaagctc ctacatcata cccatgaaat acatagtcag aaaaacctgt gacaaagatg
    12601 attatagtat cttttgaatg ttttcgtatg gtttttgcta tgtcaacgcc attacttttt
    12661 ccaagttcaa tatctaaaaa aataatatct atgtaataat tttcgtgttc tagattgtat
    12721 actaggcttt ctccatttga atattctttt atttggtagg ggtaacccca caaatccatt
    12781 tctcttgtaa tgattttatt cagcatgttt ctttgttgtt tttcatcatc acaaatacaa
    12841 atcgaagtca attttttatc tcctttcaag ttttatattt atatagttaa gtatataata
    12901 tattttgcta cctagatata taaaatgaaa taagaagtat atccagatag caaaataaaa
    12961 ttttttatat tagtatactt acacttaaaa ctattctatt aaacttattc tgaagtgcca
    13021 atgattttct tattaaaaaa tctaataacc tttcttatta taaaatacca atgattctct
    13081 tactttaaaa atgtaataac ttttcatatt atgaaatacc aatgattttc ttattataaa
    13141 aatgcaatgg ctttttacat tatgaaatat caatgatttt tttattataa agtgtcaatg
    13201 attttattac tttaaaaatg caatgacttt tcttattata aaataccaat gattttatta
    13261 ctttaaaaat ctaataacct ttcttattat aaaataccaa taattttttt gaatttctta
    13321 taagttaata gatatataag gtacatataa accccacaaa tccctataaa acctaagttt
    13381 atcaaaagag cattaacctc actattgacg attctaccat cattatttaa cataatcata
    13441 aaattaaaca gataaattaa aaaacttcct gcaataagag ggtatgaaaa tactttcttt
    13501 aattgatttc taatacagaa ttttatataa tcgttgtttg ctcctaatcg atttaaatcc
    13561 ataaaaacct gcttgtgatt tatagctata gtaacccccc ttgtatatgc aatgattcct
    13621 actgctgtaa aacatatgat tgccacatat atgaataaca ttaagaatac tgccatgttt
    13681 tttatcatat cctggctttg taaaatttta aagttaggat agtatttcca attaaggtac
    13741 agctgattgt tatctgggtc tagttttaaa tgaccttcac cacttggata ttcttcatcc
    13801 ataaaatatt ttcttccagt tgattcataa cgttttttta cgtattcatc ataaccactg
    13861 aacatgccca tttccttaga tgttcttttt ataatttctt tctttaagtc attagcaaaa
    13921 gcataggtat ctttatctac attaaatagt atagaattat tgtaattttt tatggataaa
    13981 tctttagtaa atgatttata atcctcatca ttaaggacat aggcgttcat acttctattt
    14041 tgaaaaaaag aaccattaaa tattagtttt ccagcatatc taaaggggat ttctttttct
    14101 gtgactgggc ttgatataaa tttggtatat ttataatctt gattttcatt tcctttattt
    14161 acaacagcaa gatattcacc attttttata gttattggtt tgccaattag cttggaaaaa
    14221 tctttagaat taaagaacat atcatatgag ctttttttca tatatacctt ttcgatagtt
    14281 ccatctttat gatatattga ttctacacca tcaacaatta attctataga aggaatttcc
    14341 atatagtttt gaatttttat attgtattta tctgctaatt gttcaatatc tttttttgat
    14401 acctgtttat ctctttgatt gtaagtaaaa gtataatcta tagggttatt tttaagagta
    14461 tcataaatac ctgtaaccat attaggaaca tagaatacag caaataatgc tcctgcaatc
    14521 aaaaatgaaa taacacacat gttttttact gtttgtcttc ccataaatct catcatgcta
    14581 gttgatatga tgtttttata gtatttctct ggatgttttc ctcttttagc atgtacaact
    14641 atgtatgtca taatcatata tattccaata agagagagta gataagtaaa gctccatata
    14701 gatggcagtc tataatttaa tttgtttata gttagtgaag gtacgagata tcctagaaaa
    14761 ataccgagta taataaaaat tgtacctaat ataccatacc agtcctttac atcctgtatt
    14821 ggttcagata ttctctggtc atttaatata tcaattatat ttgtacgatt aataaatttt
    14881 acagaaagta taaatataca taatgttaca aatatactaa atataaatcc atatatgaat
    14941 ccttgagttc caaaatgata tgtcatttct ctagtatcaa taatcactaa ttgaaatatt
    15001 ttccatatac caaaagatac aggaatactt aatataagac ctaaaaaaca gcatattatt
    15061 gaaattaaca atatctctga aaatagaagt ttttttaatt tcttcttatc tgttcctaga
    15121 gccatgaaaa taccaatttc cttgctttta aattttaaaa agagggaaga agcgtatgta
    15181 gtaaagacag cacatcctaa tatagcaact gagaatatca ttgtagcctg ttttctgcta
    15241 tctccaccag ttggaagaat gttttgtatt gttgggctaa aataaattag agcatatgaa
    15301 gttactaata ccacagaaag tattatacaa aagaataaag ttgtatagtt cttccaatta
    15361 taggctcgaa gtttactgta taatttatta attgtcatct tgaccgcctc ccatttcttt
    15421 taaaacttct agtaattcat ctaggaattt atgtcttgaa tttgatttta caacttcttt
    15481 ataaattatt ccatctttaa gaacaataac tcggtcgcag aagcttgctg caaagctatc
    15541 gtgtgtaacc atgagtatag tagcatctaa ttcttgcttt gcttttaaaa atgattcaat
    15601 gactgtacga caagacttac tatcaaggtt accagttggt tcatctgcta atattaagtc
    15661 tgggttgttc atgagtgctc ttgctactgc tgtacgctgt ttttctccac cagatatttc
    15721 ggcagggtat ttttttctga tatgttcaat gccaaatagc tcacatagat taactgcacg
    15781 gttttccatt tgtaagactt gaccaccttt gataatttgt ggaacacata tattttcaaa
    15841 gacactaaga ccatcaaata gcatgaagtc ttgaaataca aatcctagtt gttggtttct
    15901 aacttctgaa agttcctttt cttttaaatt tcttatatta ttatttccaa gaattatttc
    15961 tccattatcg attgggataa aacatgaaat acagtttaaa agagtagttt ttccacttcc
    16021 tgatgaaccc atgattgcga caaattctcc tttgttgatt tcaaaagaaa tattttttaa
    16081 tataggatat gttttattgc ctgttttgta tgatttacat aattggttta ctttaagcat
    16141 atatatcatc ctcactttca tttatcttgt aagtcaatga tagcatttga aagggtggag
    16201 ggatatgagc tacattgtaa tagttggatg tttttttgta atgtttgaaa atatattgtt
    16261 tgaatagtgt gagaggttat tatagttaaa ttgattatat tattagggga tttagaaggt
    16321 tattttaggt attaattgtt gtggtaaagt aatatgatgt ggaacacatt gaaggtgtag
    16381 ttagaaaaga ggagtatagt aaaagtaaaa aggttataga ataatgagtg ttgtattgca
    16441 aaaaaatgat gtaaataatg tagagtaagg ttaaaaagaa aaaatggaat aaaatgagtt
    16501 atagagcagt aagattattt aatggaatgt attgtaaata atttgaaagg atttaggggt
    16561 tattaacacc atgtaattga gaaaatatat tggagttttg aaaataaata ggttgttaaa
    16621 aacaattgaa atgtataata aaaaggttaa aaagtattat tagtataagt atgatagata
    16681 attttagaaa gtgtaggtat ttaagaatat gctgaaaagt gattgatgtg attttgaaat
    16741 attaaaatat tactatatta tagtaatgaa gttaaaataa taaaatgctg taataataaa
    16801 agtctgcagt ataataaagc actgt
//
LOCUS       DAEPXF010000080        16805 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain HB27
            SAMN13376389-rid12479423.denovo.084, whole genome shotgun sequence.
ACCESSION   DAEPXF010000080 DAEPXF010000000
VERSION     DAEPXF010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13376389
            Sequence Read Archive: SRR10518432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 16805)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16805)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001069624.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001069624.1
            Genome Coverage       :: 315x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/15/2021 01:54:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,165
            CDSs (total)                      :: 4,076
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 89
            rRNAs                             :: 10, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 10 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 19 of 69
            Pseudo Genes (incomplete)         :: 44 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 10 of 69
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16805
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13376389-rid12479423.denovo.084"
                     /strain="HB27"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="China: Hebei"
                     /collection_date="2017"
     gene            112..1485
                     /locus_tag="KRL67_002230"
     CDS             112..1485
                     /locus_tag="KRL67_002230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBH1523104.1"
                     /translation="MNEILENIDEGVHFVNKDGKTIIYNNSVQKMEKMNRHDILSNSF
                     FDIVNKMKINRSTLLEVLEKREVIKDNIQKYLDKNGKEITAINTTIPIDIKGEFVGAL
                     EISKNMTTIENLLNRNYKKNLVNKVSSRQKKGYEFDDILGESNLIKEAIAKSKKACKS
                     DASVFIYGETGTGKELFSQSIHYGSNRRNYPFIAQNCAALPESLFEGILFGTSKGGFT
                     GAIDRPGLFEQANKGTLLLDEINSMPIMLQAKLLRVLQEGYVRRVGGTKDIPIDVRII
                     ATTNESPKVILNEHKMRKDLYYRLNIIHIKIPPLRDRKEDIPLLCKKFISKYNKKLNK
                     KVVGISDEAIKILKNYDWQGNIRELENIIYCTMSMMEDEVQIETSMINLNDYYNLKEN
                     KTSNNYSLNDLEDKTLSKIISEIEEKYIYESLEKASYNITKAASILGMNRQNLQYKIK
                     KYNIKIL"
     gene            1754..2821
                     /locus_tag="KRL67_002231"
     CDS             1754..2821
                     /locus_tag="KRL67_002231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-binding protein"
                     /protein_id="HBH1523105.1"
                     /translation="MRKVRVGIWGFGAMGIGMANMILKKEGIEIVSVCSRSTSGKSMY
                     DVLGIERGERPEVIINKNYEEVFREKSVDVVLLATDSFTKKAFDKIMFLLNRKINVIS
                     TAEQMAYPQADDADLAKKMDEVAKENGVSILGTGINPGFVLDLLVLALSGTCEEVTSI
                     KAKRVNDLSPFGKSVMVEQGVGVTREEFIKGVEDKTIAGHVGFVESINMIADGLGWKL
                     DKIEQTKEPIMTTVDRKSKYGEALAGNVAGCRQCGYGYVNGEVLIEMEHPQQIIPEAE
                     GIKTGDYVSIKGIPNIDLQINPEIPGGVGTYAMIVNSIPLIINARPGLKTMLDIPVPR
                     AIMGDIRNQIEVELEEESKAN"
     gene            2942..3250
                     /locus_tag="KRL67_002232"
     CDS             2942..3250
                     /locus_tag="KRL67_002232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-ketopentanoate thiolase"
                     /protein_id="HBH1523106.1"
                     /translation="MDAKINDWVIIHNIVLTPEERAPQVPEDTKKVSLEMWVKGFIQS
                     DASIGNLVEVKTITGRLVKGNLLKVNPYYTHDYGKCIPELLQIGIQAKEILFGGVYNE
                     "
     gene            3243..4673
                     /locus_tag="KRL67_002233"
     CDS             3243..4673
                     /locus_tag="KRL67_002233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent lyase/thiolase"
                     /protein_id="HBH1523107.1"
                     /translation="MNNSTSMKDMSYQAVMGRNNEIMKNAIGLDYSSFEQEGIGFDYE
                     KMMSETGYTLQDIEAIQSQYAVGNTPIIELKNLTTLARKCAKEGKGARIFVKDEAMNA
                     SGSFKARRAATAVYHAKQMGYKGVIAATSGNYGAAVASQAAMQGLKCIIVQECYDSNG
                     VGQPEIIEKARKCEAYGAEVVQLTVGPELFYTFLVLLEETGYFNASLYSPFGIAGVET
                     LGYELAIRFREKYKKNPDIVVCTNAGGGNLTGTARGLIKAGSINTKVVGASVDLKGLH
                     MASDNQFNKKSFTTGHTGFGIPYCTWPDRSDVPRSAARPLRYMDRYVLVKQGEVFYTT
                     ELLAQLEGIERGPAGNTSLAAAFSLAQELDEDKVIVVQETEYTGAGKHICPQLTFARE
                     NGIDIKFGNPREEIAGVNLILPERPELLKCVDIDMNKIRKSFIKNCISNNHIDNIDKL
                     SNRDIEFLMEEVKSSRDFVVDVLNSL"
     gene            4751..5125
                     /locus_tag="KRL67_002234"
     CDS             4751..5125
                     /locus_tag="KRL67_002234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine aminomutase subunit alpha"
                     /protein_id="HBH1523108.1"
                     /translation="MKKREDDFEVRRKHLQELSDDELKERFWSLATQIVEPMIELGKK
                     NTTPSIERSVLLRMGFSSLEVKPILEGVMERGLIGKGAGHVVYKLAKSKNITVREAGL
                     LLVNGEYWDEVTCLFREGVESC"
     gene            5119..7323
                     /locus_tag="KRL67_002235"
     CDS             5119..7323
                     /locus_tag="KRL67_002235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892628.1"
                     /note="Presence of a B(12) (cobalamin)-binding domain
                     implies dependence on cobalamin itself, in one of its
                     several forms, or in some unusual lineages, dependence on
                     a cobalamin-like analog; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-dependent protein"
                     /protein_id="HBH1523109.1"
                     /translation="MLKENKKLDIDYILKDLDKYKPKRRGWVWREHLENLEMGPFKYK
                     DCTKPLKKSVGLPSSKYFNNIDPQPSPVITTEIASGRFEDDIRRMRMAAHHGADHLMV
                     IRTAGQSHFDGLIEGTPQGIGGVPITRKQVRAQRKALDFIEEEVGRPINYHSYVSGVA
                     GPEVAVMFAEEGVNGAHQDPQYNVLYRNINMVRSFVDACEAKKIMAFANIAQIDGAHN
                     ANATAREAWKVMPELMVQHALNSIFSEKIGIDKSNICLSTVPPTAPPAPCLRIDLPYA
                     VALRELFSDYKMRAQMNTKYMESSTREATVTHVLNLLTSVLTRADIQSTITPDEGRNV
                     PWHIYNIEACDTAKQALVGMDGLMDMIELKDVGELRDKARELKERAVLYMEEIFEVGG
                     YFESVEQGFFVDSGNYPERNGDGISRKIKGGVGEGTVYEREEDYLAPVTAHFGYNNVA
                     QYNENAIDNPSMLIDGCTFENPDKIIYIDELDDFDNVENRLKESHEYRNGTKIKPEME
                     WCADGIVLITMMLPTDKRTAEFAALEFVKKMNLQEIEVISREVMHESEGTRIEVKGRV
                     PFDIDLNNLVIPEEPKVLTDEEIRADIEEKPMKIVSATVGEDEHSVGLREIIDIKHGG
                     IEKYGIECHYLGTSVPVEKLVDAAIELNADAILASTIISHDDIHYKNMKKLHDYCVEK
                     GIRDKVMIACGGTQVTPEIAVEQGIDAGFGRNSKGIHVATFLVEKRREMNNK"
     gene            7323..8732
                     /locus_tag="KRL67_002236"
     CDS             7323..8732
                     /locus_tag="KRL67_002236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate mutase L"
                     /protein_id="HBH1523110.1"
                     /translation="MKNSKVSLKIDVLVAEIGSTTTVVNAFHDINTNNPIFLGQGQAP
                     TTVFEGGDVRNGLSGAIKDLANKLSVDDIEYNDMFATSSAAGGLKMTVHGLVYDMTVK
                     AAKEAALGAGAVIRQVTSGRIKRTDLNKIKEINPNIILIAGGVDYGERDTAIYNAEMI
                     ASMNLGIPVIYAGNIENQEEVRLIFEDTNYKLYITENVYPKIDLLNIEPTRKIIQGVF
                     EEHITTAPGMKYIKEMVNENITPTPGSVMEASKLLYKNIGDLLTLDVGGATTDVHSVT
                     DGSDYINKILVNPEPTAKRSVEGDLGVYVNMKNIVEVIGKENLQSELSIDIDAVIDNY
                     PPIPKSKEEILFVERLTKEAVVKAMLRHSGKIRNIYGTTGKVKIAEGKDLTEVRYIVG
                     TGGALTRLPSRIEILDNMFKYNKNNELLFPKEKTKILIDNDYIMASMGVLSKKYEEAS
                     LKLLLKSLNFEEERLCILG"
     gene            8717..9781
                     /locus_tag="KRL67_002237"
     CDS             8717..9781
                     /locus_tag="KRL67_002237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine/ornithine racemase family PLP-dependent
                     enzyme"
                     /protein_id="HBH1523111.1"
                     /translation="MYPRLEIDIEKLKHNAKLISQMCHERNIKISFVTKSFCAQKEIV
                     EEICSEGIDHIADSRIQNLKKLQDINLPKILIRIPMLSELEEVINYCDISFNSELGTI
                     KKLNELCKSKNIIHKIVLMFDLGDLREGYFYEEDFFNNVREIVRLKNIEIIGIATNLT
                     CYGAIIPSRENIGRLVSIAKKMEEKFGINLEIVSGGNSSSIHLLINNNMPEGITNLRI
                     GESILLGRETAYGENINGTYQDAFKLICQVVECKEKPSVPIGEIGVDAFRNKPVYEDK
                     GILKRAIIAIGKQDINIDSLIPIDTDIKILGASSDHMILDVSNTKYDYKLGDNLEFLL
                     TYGGIMSSSTSKYVSKKIIV"
     gene            9811..11232
                     /gene="nhaC"
                     /locus_tag="KRL67_002238"
     CDS             9811..11232
                     /gene="nhaC"
                     /locus_tag="KRL67_002238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HBH1523112.1"
                     /translation="MKERECRKASLFDAMIPITCLILFLSLGVLVYGSSPHVPLIGAA
                     AVAGLVAVYRLGFKWKELELSMFNSIKMAMQAILIIIIIGVLIGTWIVSGVVPCMIYW
                     GLKILSPNIFLVASTLVCAIVSLATGSSWSTMGTVGVALLGIGQSLGMPIGLIVGSII
                     SGAYFGDKLSPLSETTNLAPAMAGTDLFTHIKYMLYSTIPSLLICLVIYGVIGMKYSG
                     QALDIKQIELIRSTLDSTFNTLSPVLLLAPAIVIGLVVFKVPAIPGLIVGAVLGVLFA
                     VVFQGESMNTILDAAQNGYVSSTQIQEVDALLSKGGIMNMMSTVSLTICALSLGGILE
                     KTGMLEVVASSLLRLAKGVFGTVLCTMVTCTITNIIAGEQYLSIVIPGRMYNKEYKKR
                     GIHPKMLSRALEDSGTLTSPLVPWNTCGAYITATLGVSALTYGPYALLNIINPIVSLV
                     LIACKFKIARIEDEPETCLNFDV"
     gene            11647..12111
                     /locus_tag="KRL67_002239"
     CDS             11647..12111
                     /locus_tag="KRL67_002239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HBH1523113.1"
                     /translation="MTRISNITIAEQSEYCILAIRKTIDFIAEFSEFSKRSLEKISKY
                     LEERGVLSSGAPIVCFHNMDLAKLDVEVGFPVANYIDGKNEILQRIVPAQKIITAIDL
                     GPYELQDPTLEDLFSWANSNGYKLHGDIYYQYLNDINRPASEYLTKMMLPIV"
     gene            12409..12771
                     /locus_tag="KRL67_002240"
     CDS             12409..12771
                     /locus_tag="KRL67_002240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HBH1523114.1"
                     /translation="MKIYDILTRFYIQDIEKAIPFYENLLKEKCSLRFSYQEVGLELA
                     QIGNVLLLSGSDEALKPFIDTKSTFMVDSIDEWRNYLLDNGAVVVRDKKKVPTGYNMT
                     LRHPDGTIIEYVQHTKQE"
     gene            13367..14227
                     /locus_tag="KRL67_002241"
     CDS             13367..14227
                     /locus_tag="KRL67_002241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBH1523115.1"
                     /translation="MSYKVINVITAIDYIEEHLSEKLDLDIVANAVHYSKYHLHRTFT
                     TSVGLTMHDYIKRRKLTEAAKLLVFSKKPIIEIALIAGYESQQAFTSIFKAMYKKSPN
                     KYRKEQEFYPLQLRFVLKRDRLFPKNVLKLEKEIKLASMSDIPLWMNLVRLVIDGFPN
                     LQEEEYIYQLEQYILEERALILKLNNVAIANMVFNRETRSIDFFGIHPQYRNSDIAQV
                     FLKKVIEDFLIDTDISITTFREGDKADTGHRDMIKKLGFAEAELLVEFGYPTQKFILP
                     CVEERDNRQN"
     gene            14485..16599
                     /locus_tag="KRL67_002242"
     CDS             14485..16599
                     /locus_tag="KRL67_002242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBH1523116.1"
                     /translation="MSENKQVANCDIIASNSKYSMYEHDTELITFFPDEIAHLIEINN
                     KLDNAFKKAENLVDKLDKDYMDTKMYMVKNRGEIDPHEMFQNEQGLKQIDNYGAFMVK
                     VREKINKIKDSPYFARIDFRLEDMDDESKYYIGRFAFDYEDELIILDWRSPIASMFYD
                     YEIGKAGYDAPIGWVDGEITRKRQFKIKNGKLEYALESSINIQDDILQKELSHTSDEK
                     MKSIISTIQKEQNQIIRNDKADTLIIQGVAGSGKTSIALHRIAFLLYRFKDKISANNV
                     IILSPNKVFGDYISNVLPELGEEPLCELSFENIAEVQLDRVINFESEKDPLEINDAKW
                     VERVRFKSTLDFVKLIDDYIKQMPNKIFIPKDYTFGSFTAKSDWIQSRFEAYNRYPVK
                     KRLEKVAEDIHYKFESDNIMEEDLPRVKSILKSLNGMLTIKNTLTLYKDFFKQMNISN
                     MFVMAEKKTLEWSDVYPFIYIHAAYEGIQEDKIIRHVVIDEMQDYTPIQYAVINLLFK
                     CKKTILGDFGQLVNPNHTHTLDDMRQLYNDGELVMLNKSYRSTFEIINFTKKIQDISS
                     LEPIERHGEEPALLKCNNEQDEINKIKTEIKEFEKSDNATLGIILKTDNDAKAVYDAL
                     REEYSVHLISSESSSFTKGISITSIKMSKGLEFDEVIVPSVNNKTYYSDYDRSLLYIA
                     CTRAMHKLKLTYVGTLTQLISM"
BASE COUNT         6668 a         1854 c         3144 g         5139 t
ORIGIN      
        1 acaaatatat gataagataa atacaatagc aacattttat taattttttg ggtttttata
       61 tatttaggta ctggggtgat atagttgaac caagagatat tttttaaaat aatgaatgaa
      121 atattggaaa atattgatga aggggtacat tttgttaata aagacgggaa aactataatc
      181 tacaataatt ctgtacaaaa aatggaaaaa atgaacagac atgatatttt aagcaattca
      241 ttttttgata tagtcaacaa aatgaaaata aacaggagta cactattaga agttttggaa
      301 aaaagggaag ttatcaaaga caatattcaa aaatatctgg acaaaaatgg aaaagagata
      361 acagcaatta atactacaat accaattgat attaaaggcg agtttgtagg ggcacttgaa
      421 atatcaaaga atatgacaac gatagaaaat ttactaaaca gaaattataa aaaaaattta
      481 gtaaataaag ttagtagtag acaaaaaaag ggttatgaat ttgatgatat tttaggggaa
      541 agtaatttga ttaaagaagc aatagcaaaa tctaagaaag cttgcaagtc tgatgcttca
      601 gtatttatat atggtgaaac tggaacgggt aaagagttat ttagccagtc cattcactat
      661 ggaagtaata ggagaaacta tccatttata gcacaaaatt gtgcagcttt gccggaatca
      721 ctattcgaag gtatcctttt tggaactagc aagggaggat ttacaggagc aatagatagg
      781 ccaggcttat ttgaacaagc taacaaagga actttgttat tagatgagat aaattccatg
      841 cctattatgt tacaggctaa actattaagg gttttacaag aaggttatgt aagaagggtt
      901 ggaggaacta aggatatacc tatagatgta agaattattg caacgacaaa tgaaagtcca
      961 aaggtaattt taaacgagca taaaatgcga aaagatttat attataggct taacataatt
     1021 catataaaaa ttcctcctct tagagataga aaagaggaca tacctctatt gtgtaaaaaa
     1081 tttatttcaa agtataataa aaaattgaat aaaaaagtgg taggaatttc agatgaagct
     1141 attaaaattt taaaaaatta tgattggcaa ggtaacataa gagagctaga aaacataatt
     1201 tattgtacta tgagtatgat ggaagatgag gtacaaatag aaacaagtat gattaattta
     1261 aatgactatt ataaccttaa agagaataag acgagtaata attattcttt aaacgattta
     1321 gaggacaaaa cacttagtaa gattatcagt gagatagaag aaaagtatat atatgaatca
     1381 ctagaaaagg catcgtacaa cattactaaa gctgcatcaa ttttaggtat gaataggcaa
     1441 aatttacagt ataaaataaa aaaatataat atcaaaatct tataaataat caagataaaa
     1501 aattaaaata gaagcaaaat agaagcaaaa ccaaagtaaa gtaaaataaa ggagcaaaat
     1561 tattataaaa gagcaaaata ttttgctctt tttttatttc tcataagaaa tattattttt
     1621 tagttatgaa aatacataat aaaataatta tagtttcaac attctaataa atatttactt
     1681 aaatttaaaa gttggcatga tttatgcttt aactaattat tgagaatata taaattttta
     1741 ggagggtgta tatatgagaa aagtaagagt aggaatatgg ggatttggag ctatgggaat
     1801 aggtatggct aatatgattc taaaaaaaga aggtatagaa atagtatctg tttgtagtag
     1861 aagtacttca gggaaaagta tgtatgatgt cttaggaatt gaaagaggag aaagaccaga
     1921 agttattatc aataaaaact atgaagaagt ttttagagaa aaaagtgtag atgttgtttt
     1981 attagcaact gattcattta ctaaaaaggc ttttgataaa ataatgttcc tattaaatag
     2041 aaaaataaat gttatatcaa cagcagaaca aatggcatat ccacaagctg atgatgcaga
     2101 cttagctaaa aaaatggatg aagttgcaaa ggaaaatgga gtaagtatac ttggaacagg
     2161 aataaatcca ggatttgtat tagatttact agtactggca ctttcaggta cttgtgaaga
     2221 agttactagt ataaaagcta agagagtaaa tgatttatct ccatttggaa agtctgttat
     2281 ggtggaacaa ggtgttggag taacaaggga agaatttata aaaggtgtag aagataagac
     2341 tatagcaggt catgtaggtt ttgtagaatc aataaatatg atagctgatg gattaggttg
     2401 gaaactagat aaaatagaac aaacaaaaga gccaataatg actacagtag atagaaaatc
     2461 taagtacgga gaggcattgg ctggaaatgt tgctggttgt agacaatgtg gatatggata
     2521 tgtaaatgga gaagttttaa ttgaaatgga acatccacaa cagataatac cagaagcaga
     2581 aggtattaaa acaggagact atgtatcaat aaaaggtata ccaaacattg atttacaaat
     2641 aaatcctgaa atacctggag gagtaggtac ttatgcaatg atagtaaact ctattccatt
     2701 aataataaat gctagaccag gtcttaaaac tatgttggat ataccagtac ctagagcaat
     2761 aatgggagat ataagaaatc agatagaagt tgaattggaa gaagaatcaa aggctaatta
     2821 gtaagataat agctagatga ttagtaagat aacagctaac taattggtaa aataatagtt
     2881 aatttaaaat aacagtaaat taattagtaa gacaatagtc aattaataag gggatgaaaa
     2941 aatggatgct aaaattaatg attgggttat tatacacaat atagtattga ctccagaaga
     3001 aagagctcca caagttccag aggatactaa gaaagtatcc ttagaaatgt gggttaaagg
     3061 ttttatacaa agtgatgctt ctataggcaa cttagtagaa gttaaaacaa ttacaggtag
     3121 acttgtaaaa ggtaatttac ttaaagtaaa tccatattat actcatgatt atggtaaatg
     3181 tataccagag ttactccaaa taggaatcca agctaaagaa atattatttg gaggtgtata
     3241 taatgaataa ttcaacatca atgaaagata tgagttatca agctgttatg ggaagaaata
     3301 acgaaataat gaaaaatgca atcggattgg attactcttc ttttgaacaa gagggaatag
     3361 ggtttgatta tgagaaaatg atgagtgaaa caggatatac attacaagat attgaagcaa
     3421 ttcaatcaca gtatgctgtt ggaaatactc ctataataga gcttaaaaat ttgactacat
     3481 tagctagaaa atgtgctaaa gaaggtaaag gagctagaat atttgtaaaa gatgaagcta
     3541 tgaatgcatc tggtagtttt aaagcaagaa gagcagctac tgcagtatat catgccaaac
     3601 aaatgggata taaaggtgta atagcagcta ccagtggtaa ctatggtgca gctgtagcat
     3661 cacaagcggc aatgcaggga ttaaaatgta taatagttca agaatgttat gattctaatg
     3721 gtgtaggtca acctgaaatt atagaaaaag cgagaaaatg tgaggcatat ggagcagaag
     3781 ttgttcagtt aactgttgga ccagagttat tttatacgtt cttagtttta ttagaagaaa
     3841 caggctattt taacgcatca ttatattcac catttggaat tgcaggtgta gaaactttag
     3901 gttatgagtt agctatccga tttagagaaa agtataaaaa aaaccctgat atagttgttt
     3961 gtacaaatgc aggtggtggt aatcttactg gaactgcaag aggattgata aaagctggtt
     4021 ctattaatac taaagtagta ggagcaagtg tagatttaaa aggattacac atggctagcg
     4081 ataatcaatt taataaaaaa tcatttacaa caggacatac aggttttggt ataccatact
     4141 gtacatggcc tgataggtca gatgtaccta ggtctgcagc tagaccactt agatatatgg
     4201 atagatatgt gttagtaaaa caaggagaag ttttttatac aactgaatta ttggcacagc
     4261 tagaaggtat agaaagaggt ccagcaggaa atacatcttt agcagcagca ttctctttag
     4321 ctcaagagtt agatgaggat aaagttatag tggtacaaga aacagaatat acaggcgcag
     4381 gtaaacatat atgtccacag cttacttttg caagagaaaa tggaatagat ataaaatttg
     4441 gaaatccaag agaagaaatt gcaggagtaa acttaatatt accagaaaga ccagaattat
     4501 tgaaatgtgt tgatattgat atgaataaaa taagaaagtc atttataaaa aattgtatat
     4561 ctaataatca cattgataat attgataagc taagtaatcg agatatagaa tttttaatgg
     4621 aagaagtaaa aagttcaaga gattttgttg ttgatgtatt aaatagttta tagtaattat
     4681 tgttttttaa tttaatataa aataaaaatt cggaaaattc gattaaattg ataagaatgg
     4741 aggagtgttc atgaaaaaaa gagaggatga ttttgaagtt agaagaaaac atcttcaaga
     4801 actgagtgat gatgagttaa aagaaaggtt ttggagttta gctacccaga tagtagaacc
     4861 aatgatagaa cttggtaaaa agaatacaac accatcaata gaaaggtctg tcttgttaag
     4921 aatgggattt tcttctcttg aagtaaaacc tattttagaa ggggttatgg aaagaggtct
     4981 tataggtaaa ggagcagggc atgtagttta taaattggct aaatctaaaa atataacggt
     5041 tagagaagca ggtttgttat tagttaatgg tgaatattgg gatgaagtta catgtttatt
     5101 tagagaaggg gtagaatcat gttaaaggaa aataaaaaat tggatataga ctatatatta
     5161 aaagatttgg ataaatataa accaaaaaga agaggttggg tttggagaga acacttggaa
     5221 aatcttgaaa tgggaccttt taaatataaa gactgtacaa aacctttaaa gaaaagtgta
     5281 gggctaccat catctaagta tttcaataat atagaccctc aaccaagtcc agttattact
     5341 actgaaatag cttctggtag atttgaggat gatatacgaa gaatgagaat ggcagctcat
     5401 catggagctg accatctaat ggttatcaga actgctggac aatcacactt tgatgggctt
     5461 atagaaggaa ctccccaagg tataggtgga gttccaataa caagaaaaca agtaagagct
     5521 caaagaaagg ctcttgattt tatagaagaa gaagttggta gacctattaa ttaccattca
     5581 tatgtaagtg gtgttgctgg tcctgaagta gcagtaatgt ttgctgagga aggagtcaat
     5641 ggagcacatc aagacccaca atacaatgtt ctgtatagga atataaacat ggtaaggtct
     5701 tttgtagatg cttgtgaggc aaagaaaata atggcttttg ccaatatagc tcaaattgac
     5761 ggagcacata atgctaatgc tactgctaga gaagcatgga aggtaatgcc tgaacttatg
     5821 gttcaacacg ctttaaactc tatattctca gaaaaaatag gtatagataa aagtaatata
     5881 tgcttatcaa cagttccacc aacagcacct ccagcaccat gtttaagaat agacttacct
     5941 tatgctgttg cacttagaga gttatttagt gattataaga tgagagctca aatgaatact
     6001 aaatatatgg aatcatcaac tagagaagca acagttactc atgtattaaa cttattaaca
     6061 tcagtattaa ctagagctga catacaatca actataacac ctgatgaagg tagaaatgta
     6121 ccttggcata tatacaatat agaagcatgt gatacagcaa agcaagcttt agttggtatg
     6181 gatggtctta tggatatgat agaacttaaa gatgtaggtg aattaagaga caaagcaaga
     6241 gaacttaaag aaagagctgt attatatatg gaagaaatat ttgaagttgg tggatatttt
     6301 gaatctgtag aacaaggatt ttttgttgat tctggaaatt atccagaaag aaatggtgat
     6361 ggaatatcta gaaaaataaa aggtggagtt ggtgaaggta ctgtttatga aagagaagaa
     6421 gattatttag cacctgtaac tgctcacttt ggatacaata atgtagctca atacaatgaa
     6481 aatgctatag ataatccatc aatgttaata gatggatgta catttgaaaa tccagacaaa
     6541 ataatatata ttgatgagct tgacgatttt gataatgtag aaaatagact aaaagaatca
     6601 catgagtaca gaaatggaac taaaattaag cctgaaatgg aatggtgtgc agatggaatt
     6661 gtactgataa caatgatgct tcctactgat aaaagaacag cagagtttgc agctttggaa
     6721 tttgtcaaaa aaatgaactt acaagaaatt gaggttataa gtagagaagt tatgcatgaa
     6781 tctgaaggga ctaggataga agtgaaagga agagttccgt ttgatataga tttaaacaat
     6841 ttagtaatac cagaagaacc taaggtacta actgatgaag aaataagagc agatatagaa
     6901 gaaaaaccta tgaaaatagt ttcagctaca gttggagaag atgaacattc agttggttta
     6961 agagaaataa tagatataaa acatggaggt attgaaaaat atggtataga atgccactat
     7021 ctagggactt ctgtacctgt agaaaaattg gtagatgcag caattgaact gaatgcagat
     7081 gcaatacttg catctacaat aataagtcat gatgatattc actataaaaa catgaaaaaa
     7141 ttacacgatt attgtgtaga aaaaggtata agagataaag taatgattgc gtgtggagga
     7201 acacaagtaa cacctgaaat agccgttgaa caaggcatag atgctggatt tggaagaaac
     7261 agcaaaggca tacatgtagc cactttctta gttgaaaaaa gaagagaaat gaataataaa
     7321 taatgaaaaa tagcaaagtt tctttaaaaa tagatgtgtt ggtagctgaa attggaagta
     7381 ctactacagt ggtaaacgct ttccacgata ttaatactaa caatcctata tttttaggtc
     7441 aaggtcaagc tccaacaaca gtgtttgaag gtggcgatgt aagaaatggc ttatcaggtg
     7501 ctattaaaga tttagctaat aaattatctg tagatgatat agagtataat gatatgtttg
     7561 caacttctag tgcagctggt ggattaaaaa tgactgttca tggattagtc tatgatatga
     7621 cagtaaaagc agctaaagaa gcagctttag gggctggagc tgttattcgt caagtaacat
     7681 caggtagaat taaaagaact gatttaaata aaataaaaga aataaaccca aacataattt
     7741 taatagctgg tggagtcgat tatggagaaa gagatacagc tatttataat gcagaaatga
     7801 tagcttctat gaatctaggc ataccagtta tatatgctgg taatattgaa aatcaagagg
     7861 aagtaaggtt aatatttgaa gatactaatt ataagcttta tataactgaa aatgtatacc
     7921 ctaaaataga tttattgaat atagaaccta caagaaaaat cattcaaggt gtatttgaag
     7981 aacacataac tacagcacca ggaatgaaat acattaaaga aatggttaat gaaaatatta
     8041 ctccaactcc aggatctgta atggaagctt ctaaactttt atataaaaat attggagatt
     8101 tattaactct ggatgtaggt ggagcaacta ctgatgtaca ttctgttaca gatggaagtg
     8161 actatattaa taaaatttta gttaatcctg aacctactgc aaagagaagt gttgaggggg
     8221 atttaggtgt atatgtaaat atgaagaata tagtagaagt tataggtaaa gaaaacttac
     8281 aaagtgaact atctatagat attgatgcag taatagataa ttatcctcca ataccaaaat
     8341 caaaagaaga aatattattt gttgagaggt tgacaaaaga agctgttgtt aaagctatgc
     8401 taagacattc agggaaaata agaaatatct atggaacaac tggaaaagtc aaaattgcag
     8461 aagggaaaga tttaacagaa gtaagatata tagttggcac agggggagca cttactaggc
     8521 tccctagtag aattgaaata ttagacaata tgttcaagta caataaaaat aatgaattac
     8581 tatttccaaa ggaaaaaaca aagatattaa tagataatga ctatattatg gcatctatgg
     8641 gtgttttatc taaaaaatat gaagaagcat ctcttaagct cctgttaaaa agtttgaatt
     8701 ttgaggagga aagattatgt atcctaggtt agaaatagat atagaaaagt taaagcataa
     8761 tgcaaaatta atttcgcaga tgtgtcatga aagaaatatt aagatatctt ttgttactaa
     8821 atctttttgt gcacaaaaag aaatagtaga agaaatatgt agtgaaggaa tagaccatat
     8881 agcagattca agaatacaaa acttaaaaaa attacaagat ataaatctac ctaagatatt
     8941 gattagaatc ccaatgttaa gtgaattaga agaagttatc aattattgcg atattagttt
     9001 taactcagaa ttaggaacaa taaagaaatt aaatgaatta tgtaaaagta aaaatattat
     9061 tcataagata gttcttatgt ttgacttagg cgatttacga gaaggatatt tttatgaaga
     9121 agactttttt aataatgtaa gagagatagt aagattaaaa aatattgaaa taattggtat
     9181 agctacaaac ttaacctgtt atggagctat tataccatct agggaaaata taggcagact
     9241 ggtaagtata gcaaaaaaaa tggaagaaaa atttggaata aatctagaaa tagtatctgg
     9301 aggtaattca agttccattc atctgttgat aaataataat atgccagaag gtattactaa
     9361 tttgagaata ggcgaatcta ttctacttgg tagagaaacg gcatacggtg aaaatataaa
     9421 tggaacatat caagatgcat ttaaattaat atgccaagta gttgaatgta aagaaaagcc
     9481 atcagttcct attggagaaa taggagttga tgcctttaga aataaacctg tttatgaaga
     9541 taagggaatc ttaaaaaggg caatcatagc tataggtaaa caagatataa atattgactc
     9601 tttaattcca atagacactg atataaaaat attaggggca agttcagacc acatgatact
     9661 agatgtaagt aacaccaaat atgattataa attaggtgac aatttagaat ttttattaac
     9721 ttatgggggt atcatgtcat ctagtacgag caaatatgtt tcaaaaaaaa ttatagtgta
     9781 gtgtttaaat aattaggagg aaactatttt atgaaagagc gagaatgtag aaaagcaagt
     9841 ctgtttgatg caatgatacc aattacttgt ttgatattat ttttaagtct aggagtatta
     9901 gtgtatggtt catcacctca tgtaccacta ataggagcag cagcagtagc aggacttgtg
     9961 gctgtatata gacttggatt taaatggaaa gagttagaat taagtatgtt taatagtata
    10021 aagatggcaa tgcaagcaat cttaattata attataatag gcgtacttat aggaacttgg
    10081 atagtaagtg gagtagttcc atgtatgatt tactggggac taaaaatttt atcaccaaat
    10141 atttttttag tagcatcaac attagtctgt gcaatagtat ctcttgcgac aggttcatct
    10201 tggagtacaa tgggtacagt aggagtagct cttttaggaa ttggtcaaag tttaggtatg
    10261 ccaattggat taatcgtagg ttccataata tctggagctt actttggaga taaactatca
    10321 cctttatctg aaacgactaa cttagctcca gcaatggcag gaacagattt gtttacacat
    10381 ataaaatata tgctatattc gactatacca tcattattaa tttgtttagt aatttatggt
    10441 gtaattggaa tgaagtattc aggtcaagca ctagatataa aacaaataga attaattagg
    10501 tctacattag attctacatt taatacttta tcaccagtat tattattagc tccagcaata
    10561 gtaattggat tagttgtatt taaagttcct gctataccag ggcttatagt aggtgctgta
    10621 ttgggagtat tgtttgcagt agtttttcaa ggagaatcta tgaatacgat attagatgca
    10681 gcacaaaatg gatatgtttc gtcaacacaa atacaagagg tagatgcttt attatctaaa
    10741 ggtgggatta tgaatatgat gtccactgta tccttaacaa tatgcgcttt atctcttgga
    10801 ggtattttag aaaaaacagg tatgcttgaa gtagtagcat cttctttact aagattagca
    10861 aagggtgtat ttggaacagt attatgtact atggtaactt gtacaattac caatataata
    10921 gcaggtgaac agtatctatc tatagttata ccaggaagaa tgtataataa ggaatacaaa
    10981 aagagaggta tacatcctaa gatgttgtcg agagctttag aagatagtgg aactttgact
    11041 tctccattag taccatggaa tacatgcggg gcgtatataa cagcaacatt aggtgtatct
    11101 gcactaacat atggtccata cgctttatta aatattataa atccaatagt atctttagta
    11161 ttaattgcat gtaagtttaa aatagcaaga atagaagatg aacctgaaac ttgtttaaat
    11221 tttgatgttt gataatagat aacttatgta agaacgtagg aactacgggg gagcctgtgg
    11281 ggaattagta agacatattt tagtatgcga aattctatga atcaggaatc ttgtgacttt
    11341 agccatagga ggttcagatt actaaattta aatttatatt ttgatatttg aattgtttgt
    11401 acaaattttt tatagaaaaa gatttctttt agacttgcct aaaaattagg caagtctttt
    11461 aagtatgttc tgattataag agcactgtgt gacaagaatg atgagattat ttttgttata
    11521 ctttcgatat aagaaatgaa aggaggctat gttttatatt catatgaaat aattagtaca
    11581 cagtgaattt tggtgtaatt tggcataaaa ggattaaaca ttaaaataaa gtaatggagg
    11641 aataaaatga ctagaatatc taatataaca atagctgaac aatctgagta ttgtatctta
    11701 gcaatacgaa aaacaattga ctttatagcg gagttttcag aattttcaaa gcgaagtctt
    11761 gaaaaaattt ctaaatattt ggaggaaaga ggggttttat cctcaggggc gccaattgta
    11821 tgttttcata acatggattt agcaaaattg gatgttgaag tagggtttcc tgtggccaat
    11881 tatatagatg gaaaaaatga aattttacag agaattgttc ctgcacagaa aataataact
    11941 gcaattgatt taggacctta tgaattacaa gaccctactt tagaggactt attttcatgg
    12001 gcaaatagca atggatataa acttcatggc gatatatatt accagtattt aaatgatatc
    12061 aatcgtccag ctagtgaata tcttacgaaa atgatgttac ccattgtata gaaagttaaa
    12121 gattatcgtt tgttagttga taattagaag aacatttact gctactgcat aaattttatc
    12181 ctaaataata tcaaggtgat ttaatttatg tattctaatg taactgtaga taatcaattt
    12241 ataactaact tagtgtaatt tagatttaat ttatgtattc taatgtaatc tcaaatattg
    12301 ttaaggtcta cttaagatgc tcaaaaggct atgcttaatt tattgttata taatttgtag
    12361 agtggtataa tttagaggta atattatata gatatatagg gggaaaatat gaagatatat
    12421 gatatattaa cacgttttta tattcaagat atagaaaaag cgataccttt ttatgagaat
    12481 cttttaaaag aaaaatgttc tttgcgtttt tcctatcaag aagttggatt ggaactagca
    12541 caaataggaa atgttttact attatctggt tctgatgagg ctcttaaacc atttatagac
    12601 acaaaatcta cttttatggt tgattctatt gatgagtgga gaaattatct attggataat
    12661 ggagcagttg tagtaagaga taagaaaaaa gttcctacag gatataatat gacattgaga
    12721 catccagatg gtacaattat agaatatgtt caacatacaa agcaggagta gttaaaaata
    12781 gattttacta ctgagcaact aattagaatc tattcttaga cgtacaattt attatttttg
    12841 atattaacag attataagtc aaattataat atttcaaaat agaatagata tttatttaca
    12901 taaaagagag tgtttaaaaa tgaacttttt agttcatgaa gcactctttt ttgtatgaaa
    12961 tcaaaataca tcaatatata aaataaactg atgaagagct taaattaaaa taaaaagatt
    13021 cataacagga cccaatcaaa ctttaagata gggtaagttt gtaatcatac taaataattg
    13081 taaaaagctg tttttagtcg tataatataa atatttatca tttaattaag gaatctaaaa
    13141 ggaatctaat aagaagaaaa taataaataa ctttttagat ttactaagtg aaaagttatt
    13201 gaaaattaaa ggattagagt ctttaatcag aaagtaaaaa gtaatatatt aaatttatga
    13261 attgaaaact taaacaacaa atttgtttat aaaatagcaa gattcattaa atatagctaa
    13321 gtaataattg atataatgtt gcatggataa aaggagggaa gctgcaatgt cttacaaagt
    13381 aattaatgtc ataactgcta ttgactatat agaggagcat ttatcagaaa agctggattt
    13441 agatatagtt gctaatgctg ttcattactc aaaatatcac ctgcatagaa ctttcacaac
    13501 ctctgttggt ctaacaatgc acgattacat aaagcgaaga aagttgacag aagcagcaaa
    13561 attattggta ttttcaaaga aaccaataat tgaaattgca ttgatagctg gatatgaaag
    13621 tcaacaggca ttcacttcta tatttaaagc tatgtacaaa aaatctccaa ataaatatag
    13681 gaaagaacaa gaattttatc ctttgcagct gagatttgtt ttgaaaagag atagactatt
    13741 tcccaaaaat gtactaaagt tggaaaaaga aattaaactt gcaagtatgt ctgacatacc
    13801 actatggatg aatttagtga gattagtcat agatggattt ccaaatttgc aagaggaaga
    13861 atatatatat caattagaac agtatatttt agaagaaaga gctttaatat taaaacttaa
    13921 taatgtcgct attgcaaata tggtgtttaa tagagaaact agaagtattg atttttttgg
    13981 aatacatcca cagtatcgta attcagacat tgcacaagtg tttttgaaga aagttattga
    14041 agatttcttg atagatacag atatctcaat tactaccttt cgagagggtg acaaagctga
    14101 tacaggacat agagatatga taaagaagtt ggggtttgca gaagcagagt tgcttgtgga
    14161 atttggatat cctactcaaa aatttattct tccttgtgta gaagaaagag ataatagaca
    14221 aaattaattg tagattaaat gcaaagttta ccacatattt tttgtttgaa ttattttata
    14281 tataagagta tttctgcttt taatgtattt tgcttgtaag tgtacttatg gtaataaata
    14341 tagaaagatt tagtttttta aaaaaagaac tttaaagcta aaaagctaac atttaagtta
    14401 aatagcaaga atgattaaaa actccaaaac acaattaagt ataatgattc agtgtaagta
    14461 cttaattaac aaaggaggat ttaaatgtct gaaaataaac aagtagcaaa ttgcgatatt
    14521 attgcatcca attctaaata tagtatgtat gaacatgata cagagttaat aacttttttt
    14581 ccagatgaga ttgcacatct aatagagata aataacaagc tagacaatgc ttttaagaaa
    14641 gctgaaaatc ttgttgacaa gcttgataag gattatatgg acactaaaat gtatatggta
    14701 aaaaatcgtg gtgaaataga cccacatgaa atgtttcaaa atgaacaggg attaaaacaa
    14761 atagataact atggggcttt tatggtaaaa gttcgagaga agattaataa aattaaagat
    14821 tctccatact ttgctagaat agattttagg ctggaagata tggatgatga atctaagtat
    14881 tatattggta gatttgcatt tgattatgaa gatgagctta taatacttga ctggcgttct
    14941 ccaattgcca gtatgtttta tgattatgaa ataggaaaag caggatatga tgctccaata
    15001 ggatgggtgg atggagaaat aacaagaaaa cgacaattta aaattaaaaa tggaaaattg
    15061 gaatatgcac tagaaagttc cattaatata caagatgata ttcttcaaaa ggaacttagt
    15121 cacacttcag atgaaaagat gaagtcaatt atttcaacaa tacaaaagga acaaaatcaa
    15181 attattagaa atgataaagc tgatacactg attattcaag gggtagctgg ttctgggaaa
    15241 acatctatag cattacacag aattgctttt cttctttatc gatttaaaga taaaatatca
    15301 gcaaacaatg taattatcct atcacctaat aaagtctttg gagactatat ttccaatgta
    15361 cttcctgaac ttggagaaga gccattatgt gaattaagtt ttgaaaatat agcagaagta
    15421 caattagata gagttattaa ttttgaaagt gagaaagacc cattagaaat aaatgatgct
    15481 aaatgggttg aaagagtgcg ttttaaatct acacttgatt ttgtaaaact tattgatgat
    15541 tatataaaac aaatgccgaa taagattttt atcccaaaag actacacttt tggaagtttt
    15601 acagctaaaa gtgactggat acagagtaga tttgaggcat ataatagata tccagttaag
    15661 aagagattag agaaggtagc tgaagacatt cattataaat ttgaatcaga taatattatg
    15721 gaagaagatt taccaagggt aaaaagtata cttaaaagtt tgaatggaat gttgacaata
    15781 aaaaatactc ttacactata taaggatttc tttaaacaaa tgaatatatc aaacatgttt
    15841 gtgatggcag aaaagaaaac acttgaatgg tcagatgtat acccatttat atatattcat
    15901 gctgcatatg aaggtataca agaagataag attataagac atgttgttat tgatgaaatg
    15961 caggattata ctcctattca atatgctgta ataaacttgc tttttaagtg taaaaagact
    16021 attcttggag actttggaca attggttaat cctaatcata ctcatacact agatgatatg
    16081 agacagctat ataatgatgg ggaactagtt atgttgaaca agagttaccg ttctactttt
    16141 gagattatta atttcactaa aaagattcaa gacatatctt cattagagcc tattgaacga
    16201 catggcgaag aaccagcgtt attaaaatgt aataacgaac aagatgaaat aaataagata
    16261 aaaacagaaa ttaaagaatt tgaaaagagc gataatgcta ctcttggtat tatattaaag
    16321 acagataatg atgctaaagc tgtatatgat gcgttaagag aagaatatag tgttcactta
    16381 atatcttctg aaagttctag ctttacaaaa ggaatatcca taacttctat taaaatgtca
    16441 aaaggtttag agtttgatga agttattgta ccttcagtaa ataataaaac atattatagt
    16501 gattatgacc gcagtttgtt atatattgct tgtacacgag ctatgcacaa acttaagtta
    16561 acatatgttg gtacattaac tcaacttata agcatgtaga ttatatctta tatgcattta
    16621 ttactgtaaa tgtaaattgg gacaaagatt tatcaataaa tcaacaaaaa ataatactta
    16681 taaatttaca aaaaatgaaa gctataaatt acatttgaaa ttttataaaa ttttgtgatt
    16741 tatagctttt ataatgttaa tttcttttaa aataaagtta aactttaaat ttataaaata
    16801 aaaca
//
LOCUS       DAEPXF010000081        16538 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain HB27
            SAMN13376389-rid12479423.denovo.085, whole genome shotgun sequence.
ACCESSION   DAEPXF010000081 DAEPXF010000000
VERSION     DAEPXF010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13376389
            Sequence Read Archive: SRR10518432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 16538)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16538)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001069624.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001069624.1
            Genome Coverage       :: 315x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/15/2021 01:54:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,165
            CDSs (total)                      :: 4,076
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 89
            rRNAs                             :: 10, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 10 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 19 of 69
            Pseudo Genes (incomplete)         :: 44 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 10 of 69
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16538
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13376389-rid12479423.denovo.085"
                     /strain="HB27"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="China: Hebei"
                     /collection_date="2017"
     gene            103..1182
                     /locus_tag="KRL67_002243"
     CDS             103..1182
                     /locus_tag="KRL67_002243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HBH1523117.1"
                     /translation="MSRVKNVVELLETKGVDALYLTKKTNVNYISGFPDEEAYAVICK
                     DGNFLVTDSRYMELAEKVCKDFEIINWHNFDRSVAKAVKSVCDKVGIKKLGFERTNIV
                     FDKYEELKNLIEKDNGELIPTENIVETLRYVKDKDEIKNTRKACEIADKALEELIPHI
                     KAGVSEIELATKLEYFMKMNGAQNIGFETILISGAKTSLLHGKPSDKIIEKGDFVLID
                     YGAMYNGYISDTTRTFIVGEASEKQLEIYNLVKEAQNVGVENMKVGVHATIPDAEIRK
                     VVKKYEDYYYQGIGHGVGRDVHEEPFIGNYGDKIIEEGCIITMEPGIYFPGWGGVRIE
                     DTVLITKNGPERLTKFPKDLMILDK"
     gene            1293..1892
                     /locus_tag="KRL67_002244"
     CDS             1293..1892
                     /locus_tag="KRL67_002244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH1523118.1"
                     /translation="MYKKHKFIKNIKKAWYKIKLIDRCLIIVMAILMFQSIYNLFVNE
                     VNSQDTTTIDVVVRTTSAAIFGYFLSANFIKRPSRKYTNTDVSSNLFIDNNLSPKGQE
                     SSQNNVMNVKNTIGFTSEADNYEKKIPINNNEGFEEGETSELQIIIATVICVVALTVL
                     FIVRNFTTTTSASLGTISQMRDFVSGCVGFLLGCPSRPD"
     gene            complement(2079..2951)
                     /locus_tag="KRL67_002245"
     CDS             complement(2079..2951)
                     /locus_tag="KRL67_002245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBH1523119.1"
                     /translation="MNINYLYYFQTVCKYKNMTKAAESIHISQPSITLAIKELEKELG
                     FELFYRIGNKIELTPEGKIFLDKSKHFIKQFEDFQCDALDLGKKRKASLKIGIPTVLG
                     TFLLSKILPRFNVIYPDIELKIFEVPTFVGAKMIEESTLDFCIGIIDSDIYDDIDSKT
                     IYKTELYLVTNPKNELAKHPIISNYMLKNVPFVILSEGSYHYKIITKRLEKAKPNIIL
                     HSNQLSTIRYLLENDLASTILYKEIFQNTENLCSIPLERPITANIGVLWRRNQYISHS
                     MKLFIEYMASIHIN"
     gene            3368..4771
                     /locus_tag="KRL67_002246"
     CDS             3368..4771
                     /locus_tag="KRL67_002246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH1523120.1"
                     /translation="MKGSDLVKFELMDFLMNPFVLMFAAVITGILFGKIKFGKFNFGV
                     SGALFTGLFIGWLAYSLGNLIIAKGETAAGYKAATVMMGNGIISSDFFDFFLIIFVAA
                     VGLLAAKDMKAVLKKYGARFVILGVLITFIGGSMTYAMTLLSSDKGSSAYEVSGVYTG
                     ALTSSPGLAAALETAGKHAEDVSKEFEKASIKDKKEILKVVDPEGKLDVNTTTSLTQE
                     QIDKYIAYAEAGVGIGHAVAYPFGVLIVILGVNFLPKLFRMDLKEERRKYEKEMKEAR
                     DSVSGKTIPEVPFNIITFFLTCLAGYLVGGIHVFMGPLGYFTLGATGGSLIVSLVLGY
                     IGKIGVVNFRMEEKVLNILKQIGLVFFLAIVGLRYGGKVVDSIMTSGMHLALVAIAVG
                     VTAMMIGFLVGKYVFKLNWILLSGAVCGGMTSTPGLGAAVDALDSDDPAAGYGATYPF
                     ALLTKVILVIVLHKLPM"
     gene            4798..6342
                     /locus_tag="KRL67_002247"
     CDS             4798..6342
                     /locus_tag="KRL67_002247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA hydrolase/transferase family protein"
                     /protein_id="HBH1523121.1"
                     /translation="MNNEGAFEELSGRLRNKELCDLIMNADEAAKIIEDGMVVGVSGF
                     TPSGYPKAVPLAVSERAKSGEKIKLTVYSGASLGPEVDGAWSEAGIIERRLPYQTNST
                     LRNNINKGVVDYIDMHLSHSTQFLNYGTIPKVDVAIVEALAITEDGNIIPTSGIGNSP
                     SFIKSADKVIVEINLAKPMEMEGMADIYITENPPNRKPIEINHPKDRIGTTYIPCGLD
                     KIAGIVITNMQDKTRPLGVVDEASKKISNNIITFLREEVKAGRLSKNLLPLQSGVGSV
                     ANAVLYGLCESEFENLTCYTEVVQDSMLDLIRMGKVTMASTTSVSPSPEGLIKFEEDI
                     DFFKDKIILRPQEISNNPEIARRIGVIAMNTAIEVDIYGNVNSTHIMGSKMMNGIGGS
                     GDFARNGAITIFSTESIAKNGDISSIVPMVSHVDHTEHDVMVIVTEQGYADLRGLAPR
                     ERAIKIIENCAHPDYKEQLRDYLNRACKSGAKQTPHILDEALSWHSKFMSTGTMKKAE
                     TFKSAL"
     gene            6373..7764
                     /locus_tag="KRL67_002248"
     CDS             6373..7764
                     /locus_tag="KRL67_002248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HBH1523122.1"
                     /translation="MEKAVENFEDLSKEYINGYIERARKAQREFECYTQEQVDKIVKI
                     VGKVVYYNAEYLAKLAVEETGMGVYEDKVAKNKSKAKVIYNNLKDKKSVGIIDIDRET
                     GITKVAKPVGVVAAITPCTNPIVTPMSNAMFALKGRNAIIITPHHKAIGCSTKTVEMI
                     NEELEKIGAPENLIQILDQQSRENTRNLISSADVVIATGGMGMVKAAYSSGKPALGVG
                     AGNVQCIIDRDVDIKEAVPKIIAGRIFDNGIICSGEQSVIVAEEMFDKIMDEFKNNKG
                     FIVRDKAQKEAFRNAMFVNKSMNKDAVGQSVHTIAKIAGVEIPEDTKIIVIEADGPGE
                     EDIIAKEKMCPVISAYKYKSFEEGVAIAKANLNVEGKGHSVSIHSNTVKNIEYAGENI
                     EVSRFVINQCCATSAGGSFFNGLAPTNTLGCGSWGNNSISENLDYKHLINISRIAYYM
                     PENEVPTDEELWG"
     gene            7959..9455
                     /gene="abfD"
                     /locus_tag="KRL67_002249"
     CDS             7959..9455
                     /gene="abfD"
                     /locus_tag="KRL67_002249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybutyryl-CoA
                     dehydratase/vinylacetyl-CoA-Delta-isomerase"
                     /protein_id="HBH1523123.1"
                     /translation="MALMTGAQYIESLRKLNTKVYMFGEEVKNWVDHPMIRPSINCVA
                     ATYDLAHDPEYADLMTVTSNITGEKINRFGHLHQSVDDLIKKVKMQRLCGQKTASCFQ
                     RCVGMDAFNAVYSTTFECDKAHGTNYHDNFVKYLTYIQENDLVVDGAMTDPKGDRSLS
                     PSAQPDPDMFLHIVERREDGIIVRGAKAHQTGSINSHEHLIMPTISMTEADKDYAVSF
                     AVPSDAEGVFMIYGRQSCDTRKLEEGADVDLGNKEFGGQEALVVFDNVFVPNDRIFLC
                     GEWDFSGMLVERFAGYHRQSYGGCKVGVGDVIIGAAALAADYNGANKASHIKDKLIEM
                     THLNESLYCCGIACSSEGHKTEAGNYQIDLLLANVCKQNVTRFPYEIVRLAEDIAGGL
                     MVTMPSEKDFKSDLKVGTSGMTIGEVCNKYFKASSVASTEERMRILRFLENICLGSSA
                     VGYRTESMHGAGSPQAQRIMISRQGNINQKKELAKKIAGIKKEEALNL"
     gene            9590..9865
                     /locus_tag="KRL67_002250"
     CDS             9590..9865
                     /locus_tag="KRL67_002250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH1523124.1"
                     /translation="MTCGVRFCGGCNPRYERGKALTIIKEHFNGRVDFSIAQENVEYD
                     LLLIIGGCATLCASYQQYKVKNECVKMWDFSHIERVINVIESAICET"
     gene            9889..11196
                     /locus_tag="KRL67_002251"
     CDS             9889..11196
                     /locus_tag="KRL67_002251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA hydrolase/transferase family protein"
                     /protein_id="HBH1523125.1"
                     /translation="MSWRELYQSKLCSATEAVKQIKNGDTVVFAHCVGEPPALVEAMI
                     ENAEQYKDVEIKHMVSLGSGGYTAKGMEAHFRVNPMFVSGNVRKAIENGDGDFTPTFF
                     HEVPKLLREKRLKCDVVLAQVTPPDEHGYCSLGTSVDYTYEAIKTARTVIVQVNDQFP
                     RTYGEVVHVSEFDYIVEKSQPLFELQPAKIGEVEEAIGKNCASLIEDGSTLQLGIGGI
                     PDAVMLFLTDKKDLGIHSEMISDGTLALYEKGVINGKYKNFDKEKMTVTFLMGTKKLY
                     DFANNNPAVEVKPVDYVNHPAIIMKQHKMVSINSAIQVDLMGQVVAEAMGLRQFSGVG
                     GQVDFIRGVSMGEDGKAIIAMPSITTKKDGTVISKIVSIVDEGAPITTSRNDVDYIVT
                     EYGIAELKGKSLRERARNLINIAHPSVRESLAVEFEKRFKEKY"
     gene            11216..12334
                     /locus_tag="KRL67_002252"
     CDS             11216..12334
                     /locus_tag="KRL67_002252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybutyrate dehydrogenase"
                     /protein_id="HBH1523126.1"
                     /translation="MEALRVVPKIFYFDTFKEFNEEFKIGKNDLVITNEFIYEPYMKP
                     LGIDTNLIFQEKFGTGEPSDEMIDSMTKEMKKYNFNRIIAFGGGTIVDICKILALDVP
                     EKSIDLFEGEVSPKKIKELVVVPTTCGTGSEVTNVAIAELKSKHTKKGLAVEETYADY
                     AVLIPETVKGLPYKFFVTSSVDALIHAIESYLSPKASPFTEMYSLQAIKMIMDGYKKI
                     VDKGEEERFNHLRDFVLASNYAGIAFGNAGCAAVHALSYSIGGAFHVAHGEANYQFFT
                     EVFKMYSRKKPNGKIIKCTKILADALECDPNCDVYGELEKFLNKLIAKKALREYGMVE
                     SQIDEFTDSTIANQQRLLANNYVELSREEIREIFANLY"
     gene            12619..13539
                     /locus_tag="KRL67_002253"
     CDS             12619..13539
                     /locus_tag="KRL67_002253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-bromo-4-chloroindolyl phosphate hydrolysis
                     family protein"
                     /protein_id="HBH1523127.1"
                     /translation="MYKSENFSKKSVLINSFLSKFLKIIGWGLVSLFTLSALVLSTIS
                     IIIGWAVILPIQVCAVFLLIGGVCIFKGKRLADQISRYKKYCTIINNRNIIPVELIAE
                     NTSKSLNFIIKDVQKMIDKNYFINTYIDKRNNQIVLTNEDFIPPRYEDVAYEVKEEEK
                     TYESNEVDTIINKGMNYLTQIKEANKNIKSESMGDKIVQVEDVTSKIFDVVKHDPSKL
                     TQIQKFMDYYLPTTLKLLNSYHTLEEQGIDRENITTTMESIENTMNTIVVAFENQLDY
                     LFEDEAIDISTDITVLENMLVQEGLTSGKF"
     gene            13654..14811
                     /locus_tag="KRL67_002254"
     CDS             13654..14811
                     /locus_tag="KRL67_002254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic anion resistance protein"
                     /protein_id="HBH1523128.1"
                     /translation="MNNLDDIPVMPTLTLDPFGEESNTNDIDNSDLLMKKDEKDPEEE
                     KLSESERKMVKEFAEKIDITNTNMILQYGAGAQKKVAGFSETALKSVKTRDLGEVGDM
                     LTNLVTDLKAFSADEKEQSGFLGIFKKANNKISNLKTKYDSAEVNVDKVSKELQKHQV
                     KILKDIAMLDKMYELNLAYSKELTMYIIAGKQKLKDMKEYEMPKLREKARLSGSTEDA
                     QSVNDMVSLCDRFEKKIHDLELTRMVSLQMAPQIRLVQNSNNLMAEKIQSTIVNTIPL
                     WKNQIVLALGISHLNQAMKAQREVSNMTNELLLKNAETLKMGTIETAKESERGIVDID
                     TIKKTNQSLISTIDEVVKIQHDGRIKRQEAEVELSKIENELKSKLLEFTVK"
     gene            complement(14896..16395)
                     /locus_tag="KRL67_002255"
     CDS             complement(14896..16395)
                     /locus_tag="KRL67_002255"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HBH1523129.1"
                     /translation="MAFYFDTPSHTFSEYLLVPGFSSTDCIPANVSLKTPVTKFKKGE
                     EADIYMNIPLTSAIMQSVSDDKMAIALAKEGGISFIYGSQTIEDEAAMVARVKSHKAG
                     FVVSDSNIKPDNTLKDILDLKEKTGHSTVAVTEDGTSTGKLLGIVASRDYRISRMDLD
                     TKVSEFMTPMSSIVYANKDVTLKEANNIIWDHKLNSLPVLDDNGNLMYMVFRKDYSSH
                     KENPLELLDSSKRYVVGAGINTRDFAERVPALVEAGADVLCIDSSEGFSEWQKITLDF
                     IREKYGDTVKVGAGNVVDREGFLYLAEAGADFVKVGIGGGSICITREQKGIGRGQATS
                     IIEVAQARDEYFEKTGIYIPICSDGGIVYDHHITLALAMGADFIMLGRYFSRFDESPT
                     NKVNINGSYMKEYWGEGSARARNWQRYDLGGDKKLSFEEGVDSYVPYAGSLKDNVTLS
                     LSKVRSTMCNCGALSIPELQKNAKLTLVSSTSIVEGGAHDVMLKDNRNL"
BASE COUNT         6386 a         1948 c         3022 g         5182 t
ORIGIN      
        1 aatataaaaa aattttagaa ataaactatc tgttagataa aaagaagatt aattagtgat
       61 ataatataat taagataaaa tttattattt ggaggtaaaa tcatgagcag agtaaaaaat
      121 gtagtagaat tgttggaaac taaaggtgtt gatgcactat atctaacaaa gaaaactaat
      181 gtaaactata taagtggttt tcctgatgaa gaagcatatg cagtgatttg caaagatgga
      241 aactttttag ttactgatag tagatatatg gagcttgcag aaaaggtttg caaagatttt
      301 gaaattataa attggcataa ttttgataga agtgtggcaa aggcagttaa aagtgtatgt
      361 gataaagtgg gtataaagaa acttgggttt gaaagaacta atatagtgtt tgataaatat
      421 gaagaactta aaaatctgat tgagaaagat aatggagaat taatacctac agaaaatatt
      481 gtagagactt tgagatatgt aaaagacaaa gatgaaatca aaaatacaag aaaagcttgt
      541 gaaatagcgg ataaagcatt agaagaattg attccacata taaaagctgg agttagcgaa
      601 attgaactgg ctacaaagtt agagtacttt atgaagatga acggagcaca aaatatagga
      661 tttgaaacta tactgatatc aggagcaaag acttcattgc ttcatggtaa gcctagtgat
      721 aagattatag aaaaaggtga ttttgtactt atagactatg gtgctatgta taatggttat
      781 atatctgata caactagaac ttttatagtt ggagaagctt ctgaaaagca attagaaata
      841 tataatttag ttaaggaagc tcaaaatgta ggggttgaga acatgaaagt tggtgtacat
      901 gcgacaattc cagatgctga gataagaaaa gttgtcaaaa aatatgaaga ctattactat
      961 caaggtatag gacatggagt cggtagagat gttcatgaag aacctttcat tggcaattat
     1021 ggtgataaaa tcatagaaga aggatgtata ataacaatgg aaccaggtat ttatttccct
     1081 ggatggggtg gagttagaat agaagatact gttttgatta ctaaaaatgg tccagaaaga
     1141 cttactaaat tccctaaaga tttgatgatt ttagacaaat aatttaattt atatattttt
     1201 ttacataaaa tataacataa tctcactatt aagccctatt tacatattat agtaaagagg
     1261 gcttaatttt attgtgagag gtgatagtat atatgtataa aaaacataaa tttataaaaa
     1321 atataaaaaa agcctggtac aaaatcaaac taattgatag atgtcttata attgtaatgg
     1381 caatactaat gtttcaatct atatataatt tatttgttaa tgaagtaaat tcacaagata
     1441 ctacaactat tgatgtagta gtaagaacca cttcagcagc gatatttgga tattttttaa
     1501 gtgctaattt tataaagaga ccttcaagga agtatacaaa tactgatgta tcttcaaatc
     1561 tttttataga taataattta tccccaaaag gtcaagagtc aagtcaaaat aatgtaatga
     1621 atgtcaaaaa taccattgga tttacttcag aagctgataa ttatgaaaag aaaataccta
     1681 ttaataataa tgaaggtttt gaagaaggag aaactagtga attgcaaatt attatagcca
     1741 ctgtaatttg tgtagtagca ttaacagtac tttttattgt aagaaatttt acaactacaa
     1801 catcagcttc tttaggtact atttcacaga tgagagattt tgtctcagga tgtgtgggtt
     1861 ttttgctagg ttgtccaagt agacctgatt aaaaaagtat tatgaataaa aggtatttta
     1921 aaaagtatca gtataataat atttagattt aaatgtatac aattataatt ctataacgtt
     1981 ctatattgca tttttatact gatactctaa actatactat ttattaaaat attttaacat
     2041 tgtatttaaa caaaagatta tatctgtaca atataaattt agtttatatg gatagatgcc
     2101 atatactcaa taaaaagttt catactatga gatatgtatt gatttcttct ccaaagaact
     2161 ccaatatttg cagtaattgg acgttctaat ggtattgaac agagattttc tgtattttga
     2221 aatatttctt tatacaatat tgttgaagcc aagtcatttt ctagtaaata acgtatggta
     2281 gaaagttggt ttgaatgtaa aattatattt ggcttagctt tttcaagtct ttttgtaata
     2341 atcttatagt gataagaacc ttcagataag attacaaaag gtacattttt taacatataa
     2401 ttcgatataa tgggatgttt tgccaattca tttttgggat ttgtaactaa gtacaattct
     2461 gttttataga ttgtctttga atctatatca tcataaatat cactgtcaat tataccaata
     2521 caaaaatcaa gtgtagattc ttcaatcatt ttagctccaa caaatgttgg aacttcaaaa
     2581 attttaagct ctatatctgg ataaattaca ttaaatctag gaagtatttt tgaaagcaaa
     2641 aacgtaccta aaactgtagg aatacctatt ttgagagaag cttttctctt ttttcctaaa
     2701 tcaagtgcgt cacattgaaa atcctcaaat tgttttataa aatgttttga cttgtctaaa
     2761 aaaatttttc cttcaggcgt taactctatt ttattaccta ttctgtaaaa aagttcaaag
     2821 ccaagttctt tttctaattc tttaattgct aatgttatgg atggttgaga aatatgaata
     2881 ctttccgcag ccttagtcat gtttttatat ttacagacag tttgaaaata gtataaatag
     2941 tttatattca tttggtatta gttcctttca tttacgtatt agtgacaagt atataactaa
     3001 caatagtata agttttgtat attgttgtaa aaaaatagaa tagttttaaa ctatcatagc
     3061 ataagttttt gatattttca atctattaag aatagtgcta ttattaaatt aacaaaagag
     3121 aatagctggt gtagcttaga gtaaatcaat ctatattaca gttatatcaa cgaatattaa
     3181 aaaattatct attaaaaaat aaaaattatt tttacaatta aattgttaat taaatcgtaa
     3241 agaaaaaaat gatattaaca agataaaatt tttttaatca gaaatgttaa tttaataaca
     3301 aaatagagtc ttggggagaa aggagaaata tattttttaa taatatttgt taatttaata
     3361 acgaaaaatg aaagggagtg atttagtgaa gtttgaatta atggattttt taatgaatcc
     3421 ttttgtcctt atgtttgctg cagtaattac aggaatttta tttggaaaaa ttaaatttgg
     3481 gaagtttaat tttggggtat caggagcatt atttacagga ttatttattg gatggcttgc
     3541 atatagcttg gggaatctaa taatcgcaaa aggggaaaca gcagcaggat ataaggcagc
     3601 aactgtaatg atgggaaatg gaattatttc ttctgacttc tttgatttct ttttaataat
     3661 atttgttgca gcagttggtc tattggcagc aaaagatatg aaagctgtat taaagaagta
     3721 tggagcaaga tttgtaattt taggagttct tataactttt ataggtggct ctatgactta
     3781 tgcaatgact ttactatcaa gtgataaagg aagtagtgct tatgaagttt ctggtgttta
     3841 cacaggagca ttaacaagtt cgcctggatt agctgctgct ttagaaacag ctggtaaaca
     3901 tgcagaagat gtatcaaaag aatttgaaaa agcttcaata aaagataaaa aagaaatttt
     3961 aaaagtagtt gacccagaag gcaaattgga tgtgaataca actacatcac tgactcaaga
     4021 gcaaatagat aagtacattg catatgctga agctggtgtt ggtataggtc atgcagtcgc
     4081 atatcctttt ggagttttaa tagtaatctt aggtgtaaat ttccttccaa aattatttag
     4141 gatggacctt aaagaagaaa gaagaaaata tgagaaggaa atgaaagaag caagagatag
     4201 tgtttctggt aaaacgatac cagaagttcc atttaatata ataacatttt tcttgacatg
     4261 tttagctgga tacttagttg gaggaataca tgtatttatg ggaccactag ggtacttcac
     4321 actaggagca acaggaggtt cactaattgt atctctagtt cttggatata taggtaagat
     4381 aggggtagta aatttcagaa tggaagaaaa ggttttaaat attttaaaac aaatagggct
     4441 agtattcttc ttagcaatag ttggattaag atatggtgga aaagtagtag attcaattat
     4501 gacatctggt atgcacctag ctttagtagc aatagcagta ggtgttacag ctatgatgat
     4561 aggattctta gttggtaaat atgtatttaa attaaattgg attttactat ctggagctgt
     4621 ctgtggtggt atgacatcaa caccaggtct tggagctgct gttgatgctc ttgatagtga
     4681 tgacccagca gcaggttatg gtgctacgta tccatttgca ttattaacta aagttatatt
     4741 ggttatagtt ttacacaaac ttccaatgta aataagattt tatatggggg gatatttatg
     4801 aataatgaag gagcatttga agaattaagt ggaagattaa gaaataaaga attgtgtgat
     4861 ttaataatga atgcagatga agctgctaaa attatagagg atggcatggt tgttggagtt
     4921 agtggattta caccttcagg ctatccaaaa gcagtaccat tagcagtttc agaaagagct
     4981 aaaagtggag aaaagataaa attaaccgta tattcaggag catctttagg tccagaagtt
     5041 gatggagcat ggtcagaagc aggtataatt gaaagaagac ttccttatca gactaattca
     5101 acacttagaa ataatattaa taaaggtgtt gttgattata tagatatgca cctaagtcat
     5161 tctactcaat ttttaaatta tggaactata cctaaagtag atgtagctat agtagaagca
     5221 cttgcaataa ctgaagatgg gaatattata ccaactagtg ggataggtaa ttctccatct
     5281 tttataaaaa gtgcagataa agtaatagta gagataaatc ttgcaaaacc aatggaaatg
     5341 gaaggtatgg cagatatata tattacggaa aatccaccaa atagaaaacc aatagaaata
     5401 aatcatccta aagatagaat tggaacaaca tatatacctt gcggtttgga taaaattgca
     5461 ggaatagtaa ttaccaatat gcaagataag acccgtccat tgggtgttgt agatgaagct
     5521 tcgaaaaaaa tatcaaataa tattattact tttttaagag aagaagtaaa ggcaggcaga
     5581 ttatcaaaga atttacttcc tcttcaatct ggtgtaggaa gtgtagcaaa tgcagtatta
     5641 tatggattat gtgaatctga atttgaaaat ttaacttgtt atacagaagt tgtacaggat
     5701 tctatgttgg atttaataag aatgggtaaa gttacaatgg catctactac atctgtaagt
     5761 ccatctccag aaggattaat aaaatttgaa gaagatattg atttttttaa ggataagatt
     5821 atattaagac ctcaagaaat tagtaataat ccagaaatag ccagaagaat tggtgtaatt
     5881 gcaatgaaca ctgcaattga agttgatatt tatggaaatg taaattctac tcacataatg
     5941 gggtcaaaga tgatgaatgg aattggtggt tctggtgatt ttgctagaaa tggagcaata
     6001 actatattta gtactgagtc aattgcaaaa aatggtgata tatcatcaat agtacctatg
     6061 gtatctcacg tagaccacac tgaacatgat gtaatggtaa ttgtaacaga acaaggatat
     6121 gctgatttaa gaggtcttgc acctagagaa agagctataa aaataataga aaattgtgcc
     6181 catccagatt ataaagaaca acttagggat tatttaaata gagcttgtaa aagtggagca
     6241 aaacaaacac ctcacatatt ggacgaagct ttatcatggc atagtaaatt tatgagtaca
     6301 ggtacaatga aaaaggctga aacttttaaa agtgcattat aaaatattta ataaattgga
     6361 ggtagatttt aaatggaaaa agcagtggaa aactttgaag atttaagtaa ggaatacatt
     6421 aatggttata ttgagagagc aagaaaagct cagagagagt ttgaatgtta tactcaagaa
     6481 caagtagata aaatagtaaa aatagttgga aaagtagtgt attataatgc tgaatattta
     6541 gctaaacttg ctgtagaaga gactggaatg ggagtatatg aagataaggt agctaaaaat
     6601 aaaagcaaag caaaagttat atataacaat ttaaaagata aaaaatcagt tggtataatt
     6661 gatatagata gagaaactgg gataacaaag gtagctaaac cagttggagt agtagcggca
     6721 ataactcctt gtacaaatcc aattgttaca cctatgagca atgcaatgtt tgcacttaag
     6781 ggaagaaatg caattattat cacacctcat cataaagcaa ttggatgtag tacaaaaact
     6841 gtagagatga ttaatgaaga attagaaaaa ataggagctc cagaaaattt aatacaaata
     6901 cttgaccaac aatcaagaga aaatacaaga aatttaattt catcagcaga tgtagtaatt
     6961 gccactggag gaatgggaat ggtaaaagct gcgtactcaa gtggaaaacc agcacttggt
     7021 gtaggggcag gaaatgttca atgtatcata gatagagatg tagacattaa agaagcagta
     7081 cctaaaatca tagcaggaag aatttttgat aatggtatca tttgttcagg tgaacagtca
     7141 gtaatagtag cagaagaaat gtttgataaa ataatggatg aatttaaaaa taacaaaggt
     7201 tttatagtta gagataaagc acaaaaagaa gctttcagaa atgctatgtt tgtaaataaa
     7261 tctatgaata aagatgcagt aggtcaatca gttcatacaa ttgcaaaaat tgcaggtgtt
     7321 gagataccag aagatacaaa aataatagta atagaagctg atggaccagg agaagaggat
     7381 atcattgcaa aagaaaaaat gtgtccagtt atatctgcat acaagtataa aagttttgaa
     7441 gaaggtgtag ctattgcaaa agcaaattta aatgtagaag gaaaaggaca tagtgtatct
     7501 atacattcaa atacagttaa aaatatagaa tatgcaggag aaaatataga agtatcaaga
     7561 tttgttatta atcaatgttg tgctactagt gctggaggaa gtttctttaa tggtcttgca
     7621 cctactaata ctttaggttg tggttcttgg ggaaataaca gtatctctga aaatcttgat
     7681 tataagcatc taattaatat ttctagaatt gcttattata tgccagaaaa tgaagtccct
     7741 acagatgaag aactttgggg ataaaattta gacaaaaaaa atattcattc taaattaaag
     7801 taggaatata ttttaattta gaatgaattt aagacataaa ttaaatttaa attagtttat
     7861 tgaactaaat ttaaaatatt aagagacata tagaagaagg aagtattata atttaaaata
     7921 aataaaaaaa tctataaaag agaaggagtg gttttattat ggcattaatg acaggagctc
     7981 aatatattga aagtttgaga aaattaaata ctaaggttta tatgtttggt gaagaggtta
     8041 agaattgggt tgaccatcct atgattagac catctataaa ttgtgtagca gcaacttatg
     8101 acttagctca tgacccagaa tatgcagatt taatgacagt tacttcaaat atcacaggtg
     8161 aaaaaataaa tagatttggt catcttcatc aaagtgttga tgatttgata aaaaaagtaa
     8221 aaatgcaaag actatgtggt caaaaaacag cttcttgttt ccaaagatgt gttggtatgg
     8281 atgcatttaa tgcagtgtat tcaacaacat ttgagtgtga taaagcccat ggaactaatt
     8341 atcatgataa ttttgtaaaa tacttaactt atatacaaga aaacgattta gtagttgatg
     8401 gagctatgac tgaccctaaa ggagatagaa gtttatcacc tagtgctcaa cctgacccag
     8461 atatgttcct tcacatagta gaaagaagag aagatgggat aattgtaaga ggagcaaagg
     8521 cacatcaaac tggttcaata aattcacatg agcaccttat aatgcctact ataagtatga
     8581 ctgaagcaga taaagactac gctgtatcat ttgcagttcc ttcagatgct gaaggagtgt
     8641 ttatgattta tggaagacaa tcttgtgaca ctagaaaatt agaagaaggt gcagatgttg
     8701 atttaggaaa taaggagttt ggaggtcaag aagctctagt agtatttgat aatgtatttg
     8761 ttccaaatga tagaattttc ttatgtggag aatgggattt ctctggtatg ttagtagaga
     8821 gatttgcagg atatcataga caaagttatg gtggatgtaa agtaggagta ggtgatgtaa
     8881 taataggagc agcagcttta gcagctgatt ataatggagc aaacaaagct tcacatataa
     8941 aagataaact aatagaaatg actcacctta atgaaagctt atactgctgt ggtatagcat
     9001 gttcatcaga aggtcacaaa acagaagctg gaaactatca aatagattta cttcttgcaa
     9061 atgtgtgtaa acaaaatgta actagatttc cttatgaaat agtaagactt gcagaagata
     9121 tagctggagg gcttatggtt actatgcctt cagaaaaaga ctttaaatca gaccttaaag
     9181 ttggaacaag tggaatgact attggcgaag tgtgtaacaa gtattttaaa gcttcttcag
     9241 tggcttcaac agaagaaaga atgagaatat taagattctt agaaaatata tgtcttggtt
     9301 catctgctgt tggttataga actgagtcaa tgcatggggc aggttcacca caagctcaaa
     9361 gaataatgat ttcaagacaa ggaaacataa atcaaaagaa agaattagct aagaaaattg
     9421 caggaataaa aaaagaggaa gctttaaatt tataaaaagt tgatatataa agggaaatta
     9481 tatattaatg tgtattaatt aatgaaaaaa tatgtagtat aagagataat tataaaatta
     9541 tggtggtttt tagctaagta aagaaatttt ttataaggag aatttacaca tgacctgtgg
     9601 agttagattt tgtgggggct gtaaccctag atacgaaaga gggaaggctc ttacaataat
     9661 caaagagcac ttcaatggca gggtagattt tagtatagca caagagaacg tcgagtatga
     9721 tttgctactt ataattggag gatgcgcaac tctatgcgca tcctatcaac aatataaagt
     9781 gaaaaatgaa tgtgttaaga tgtgggattt tagccatata gaaagagtta taaatgtgat
     9841 tgaatcagca atttgtgaaa cttaaaaaag ctaaatgggg ggatttcatt gagttggcga
     9901 gaattgtatc aaagtaaatt atgtagtgct acagaagctg taaaacagat taaaaacgga
     9961 gatactgtag tatttgcaca ttgtgtaggt gaacctcctg cactagtaga ggctatgatt
    10021 gaaaatgctg aacaatataa agatgttgag attaaacata tggtaagctt gggtagtggt
    10081 ggatatactg ctaaaggaat ggaagctcat tttagagtaa atccaatgtt tgtaagtgga
    10141 aatgtaagaa aggcaattga aaatggagat ggtgatttta cacctacatt cttccatgaa
    10201 gtaccaaagt tgcttagaga aaaaagatta aaatgtgatg ttgttttagc acaggtaact
    10261 ccaccagatg aacatgggta ttgtagctta ggaacatcag ttgattatac ttatgaagcc
    10321 attaaaactg ctagaacagt aattgttcaa gtgaatgacc agtttcctag aacctatggt
    10381 gaggtagtcc atgtatcaga gtttgactat atagtggaaa aatcacaacc tttatttgaa
    10441 ctacaacccg caaagattgg agaagtagaa gaagcaattg gtaaaaattg tgcttcatta
    10501 attgaagatg gttctacttt acagctaggg attggtggga taccagatgc agttatgtta
    10561 tttttaactg ataaaaagga tttagggatt cattcagaaa tgatttcaga tgggactttg
    10621 gccctttatg aaaaaggtgt tataaatggt aaatataaaa attttgataa agaaaaaatg
    10681 acagttacct tcttaatggg tactaaaaaa ttatatgact ttgcaaataa taaccctgca
    10741 gtagaggtaa aaccagtaga ctatgtaaat catccagcaa ttataatgaa acaacataag
    10801 atggtttcta taaattctgc tatacaagtt gatttaatgg gacaggtagt tgcagaggct
    10861 atgggactta gacaatttag tggtgttgga ggtcaagttg actttatacg tggagtgtct
    10921 atgggtgaag atggaaaggc aattatagct atgccttcaa taactacaaa aaaagatggt
    10981 acagtaattt caaaaattgt atctattgtg gatgaaggtg cacccataac aacatcaaga
    11041 aatgatgttg actatattgt aacagaatac ggtatagcag aattaaaagg aaaatctctt
    11101 agagaaagag caagaaattt aataaatata gctcatccaa gtgtaagaga aagtctagca
    11161 gtagaatttg aaaagagatt taaggagaaa tattaataat agaggggtgt taataatgga
    11221 agcacttaga gtagttccaa agatatttta ttttgataca tttaaagagt ttaatgaaga
    11281 atttaaaata ggaaaaaatg atttagtaat tactaatgag tttatatatg agccatatat
    11341 gaaacctctt ggaatagata caaatttaat ttttcaagaa aagtttggaa caggtgaacc
    11401 atctgatgaa atgatagact ctatgacaaa ggaaatgaag aaatacaatt ttaatagaat
    11461 tattgcgttt ggaggaggaa caatagttga tatatgtaag atattagcac ttgatgttcc
    11521 agaaaagtct attgatttat ttgaaggaga agtatcacct aaaaaaataa aagagttagt
    11581 agtagttcct acaacatgtg gtacaggtag tgaagtaaca aatgtagcta ttgcagagtt
    11641 aaaatctaaa catactaaaa aggggcttgc agtagaagaa acatatgcag actatgcagt
    11701 gttaattcca gagacagtaa aagggttacc ttacaaattc tttgttacaa gttcggtgga
    11761 tgctcttata catgctattg agtcttatct ttctccaaaa gcaagtccat ttacagagat
    11821 gtattctcta caagcaataa aaatgattat ggatgggtat aagaagatag ttgataaagg
    11881 agaagaagag agatttaatc atcttagaga ctttgtactt gcatcaaatt atgcaggaat
    11941 tgcttttgga aatgctggtt gtgcagcagt tcatgcacta tcttattcaa taggtggagc
    12001 tttccatgtt gcacatggtg aagcaaatta tcagttcttt actgaagtat ttaagatgta
    12061 ttctagaaaa aaacctaatg gaaaaattat aaaatgtaca aaaattttag cagatgccct
    12121 tgaatgtgac cctaattgtg atgtttatgg agagctagaa aagtttttaa ataaattaat
    12181 agctaaaaaa gctttaagag agtatggaat ggtagaaagt cagatagatg aatttacaga
    12241 tagtacaata gccaatcaac aaagattact tgctaataat tatgtagagc ttagtagaga
    12301 agaaataaga gagatatttg caaatcttta ttaatacaat tttttattta tatgtatagt
    12361 caacataaac aatgctaatt atatttgtgt acaaataaaa atgagggaac ttaaatttaa
    12421 ttaaagttaa gttctcttat ttttatatgg agttaaatat aagtatattt ttaatttaaa
    12481 atatgcttat atttaagtct attactttat ttgaaacaat ttgtagaatt tcaaattcct
    12541 tgacagtaat aagtaagtaa ttgtataatc aaactgcaat atattaataa tattagtgaa
    12601 aatgaaagga tataaaatat gtataaatca gaaaattttt caaaaaaatc tgtattaata
    12661 aattcatttt taagtaaatt tttaaaaata attggatggg gtttggtaag cttatttaca
    12721 ttatcagcct tagtactttc aactatttcc attataattg gatgggctgt aatacttcct
    12781 attcaagtat gtgcagtatt tttacttata ggaggagttt gtatttttaa aggtaagaga
    12841 ttagcagacc aaatttctcg ttataaaaaa tattgtacaa ttataaacaa tagaaatatt
    12901 attccagtag aacttattgc agaaaacaca tctaaatcat taaattttat aataaaagat
    12961 gtgcaaaaga tgatagacaa aaactacttt ataaatactt atattgataa aagaaataat
    13021 cagattgtgt taacaaatga agattttatt ccaccaagat atgaagatgt agcatatgaa
    13081 gtcaaagaag aagaaaagac ttatgaatcc aatgaagttg atacaattat aaataaagga
    13141 atgaattatc taacacaaat aaaagaagct aataaaaata ttaaatcaga atcaatgggc
    13201 gataaaattg tacaagtaga agatgttaca agtaaaatat ttgatgttgt aaaacatgac
    13261 ccatcaaaac ttactcaaat acaaaaattt atggattatt atcttccaac cactttaaaa
    13321 ctattaaatt catatcatac attggaagaa caaggaattg atagagaaaa tataacaaca
    13381 actatggaaa gtattgaaaa tacaatgaat acgattgtag tagcttttga aaatcaattg
    13441 gactatctgt ttgaagatga agccattgat atttctacag atattactgt tttagagaat
    13501 atgttagtcc aagaaggatt aacgagtggt aaattttaac ttcaatagaa aaaatattct
    13561 gttattttta aacaattaat tagtcataaa gtataaaata tgtattatat aagttaatca
    13621 gtactttatg aaggaagagg agagaaagat acaatgaata atttagatga tataccagtt
    13681 atgccaactt tgacacttga cccatttgga gaagaatcaa acactaatga tatagataat
    13741 tctgacttat taatgaaaaa agatgaaaaa gacccagaag aagaaaaact gtctgaatct
    13801 gaacgcaaga tggtaaagga atttgcggaa aaaatagata ttacaaatac aaatatgatt
    13861 ttgcaatatg gagctggtgc tcaaaaaaag gtagcaggct tttctgaaac agctttaaag
    13921 agtgtaaaaa caagagattt aggagaagtt ggagatatgc ttacaaatct agttactgat
    13981 ttaaaagcat tttcagcaga tgaaaaagaa caaagtggtt ttttgggaat ttttaaaaag
    14041 gcaaataata aaatttcaaa tctaaaaact aagtatgata gtgcagaggt aaatgtggat
    14101 aaagtaagta aagagcttca aaagcatcag gttaaaatat tgaaagatat agcaatgctt
    14161 gataagatgt atgaattgaa cttagcttac tcaaaagagc ttactatgta tattatagca
    14221 ggaaaacaaa aattgaagga tatgaaagaa tatgagatgc caaagttgag agaaaaagct
    14281 cgtttatctg gttcaacaga agatgctcaa tctgtaaatg atatggtttc actttgtgac
    14341 agatttgaaa agaagattca tgacttagaa ttaactagaa tggtatcgct tcaaatggct
    14401 cctcagataa gattggttca aaatagtaat aatctgatgg cagaaaagat tcaatcaact
    14461 atagtaaata ccattccatt atggaaaaat caaatagtgc ttgctcttgg aatttctcac
    14521 ttaaatcaag ctatgaaagc acagcgtgaa gttagtaata tgacaaatga gcttttatta
    14581 aagaatgcag aaactttgaa gatgggaaca atagaaacag caaaagaatc agaaagagga
    14641 atcgtagata tagacacaat taagaaaact aatcaatctc taatatctac tattgatgag
    14701 gtagttaaga tacaacatga tggtagaata aagagacaag aagcagaagt tgaattatca
    14761 aaaatagaaa atgaacttaa aagcaagctt cttgaattta cagttaaata aaaattatac
    14821 taagataaaa agagactgtc tcaaaataga gataagtctc tttttcattg tttaaatgat
    14881 taaagtatat ttatattata aatttctatt atcttttaac ataacatcat gagctccacc
    14941 ttcaactata ctagtagaag atacaagtgt aagtttagcg tttttttgaa gttcaggaat
    15001 gctaagtgct ccacagttac acatagtaga acgaacttta cttaaagaaa gagttacatt
    15061 gtctttcaaa gaaccagcat aaggaacata agagtcaaca ccttcttcaa aagaaagttt
    15121 tttatctcca ccaaggtcat atctttgcca gtttctagca cgagcagaac cttctcccca
    15181 atactctttc atataactac catttatatt aactttgtta gttggactct catcaaaacg
    15241 agagaagtat ctaccaagca ttataaaatc tgcacccata gctaaagcta aagttatatg
    15301 atggtcataa acgataccac catcagagca aattggaata taaatacctg ttttttcaaa
    15361 gtattcgtcc ctagcttgag caacttcaat tatagaagta gcttgtccac gtcctatacc
    15421 tttttgttca cgagttatac atatagaacc accaccaata ccaactttaa cgaagtcagc
    15481 accagcttca gctaggtaaa gaaatccctc gcggtcaact acatttccag ctcctacctt
    15541 aactgtatca ccatattttt cacgaatgaa atcaagtgtt attttttgcc actcagaaaa
    15601 accttctgaa gaatctatac ataaaacatc agcaccagct tcaactaaag caggaacacg
    15661 ttcagcaaag tctctggtat taatacccgc tccaactaca tatctcttag aagaatctaa
    15721 aagttcaagt ggattttctt tatgagaaga gtaatcttta cggaatacca tatacataag
    15781 attaccatta tcatcaagta caggaagaga attaagtttg tggtcccata taatgttgtt
    15841 tgcttccttt aaagtaacat ctttgttagc ataaactata gatgacatag gagtcataaa
    15901 ttcactaact ttagtatcta aatccatacg acttatacga taatcacgac ttgcaacaat
    15961 acctaataat ttaccagttg aagttccatc ttcagtaaca gctactgtag aatgtccagt
    16021 tttttctttt aagtcaagta tgtcttttaa tgtgttatca ggtttaatat ttgaatcact
    16081 tacaacaaaa ccagctttgt gggatttaac acgtgctacc atagcagctt catcttcaat
    16141 agtttgagaa ccgtatataa atgaaatacc accttcttta gcaagtgcaa tagccatttt
    16201 gtcatcagaa acagattgca taatagcaga agtcaaaggt atattcatat aaatatctgc
    16261 ttcctcgccc tttttaaatt tagtaacagg tgtttttaaa cttacattag caggtataca
    16321 atcagtagaa gagaaacctg gtacaagaag gtattcactg aaagtatgtg atggtgtatc
    16381 aaaataaaac gccatttttt aatcactcct tatattttta tactttttat aattgatagt
    16441 attaattata acttattctt taattttatt aaagtatttt gtcaaatatt gagatttttg
    16501 aaaaatttaa actattttaa ttgaatttgt ttaatata
//
LOCUS       DAEPXF010000082        16250 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain HB27
            SAMN13376389-rid12479423.denovo.086, whole genome shotgun sequence.
ACCESSION   DAEPXF010000082 DAEPXF010000000
VERSION     DAEPXF010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13376389
            Sequence Read Archive: SRR10518432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 16250)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16250)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001069624.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001069624.1
            Genome Coverage       :: 315x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/15/2021 01:54:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,165
            CDSs (total)                      :: 4,076
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 89
            rRNAs                             :: 10, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 10 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 19 of 69
            Pseudo Genes (incomplete)         :: 44 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 10 of 69
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16250
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13376389-rid12479423.denovo.086"
                     /strain="HB27"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="China: Hebei"
                     /collection_date="2017"
     gene            207..1397
                     /gene="dltD"
                     /locus_tag="KRL67_002256"
     CDS             207..1397
                     /gene="dltD"
                     /locus_tag="KRL67_002256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltD"
                     /protein_id="HBH1523130.1"
                     /translation="MRKLIYFITPFIIGVVFLFGLDKFLDSKTDELLREKNLLPIMDD
                     TLSDIKDKGVTANNHFLREKDIMILGSSELSNSTKQHPKYYFNTNRSKNKVFAIGRAY
                     TQTLQDAAILGSMNPNIDNKKVVLLISMQWFMEKDGVTSHHYQSRFSPIQFYRFLDNP
                     KISKQNKIEYAKKSSKLLWGSDEYKAEALYAKLYEPKTLPEKAEKVLLEPYFQGRKYC
                     IALKEKGILYKRLIKLDKKRATKRKRPINWSYERKKAIEDAKKRVGKNPLNIDKYYYK
                     EHFKDGIEQYKGRDKDVNLLTSKEFENYKLMLNVCTDLGIKPVVVLIPSMDKFYNLTG
                     ISEKERNEYYDKAQKIAESKGFEVLNLKDKGSDKYYLRDVMHLGTKGWVDVCERLFKI
                     FKEQ"
     gene            1518..3032
                     /locus_tag="KRL67_002257"
     CDS             1518..3032
                     /locus_tag="KRL67_002257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase"
                     /protein_id="HBH1523131.1"
                     /translation="MKIIEGIKKYSNTDRTALMCNGDKLSYKDLNEYSDAISVFLKDV
                     YKEEDTPIVIYGNKENMIMACMIGALKSGRAYVPLDISFPIDRVFEVTKEIKPKVIFN
                     FSDERDFGDIDVIDMDKLKDIINEYQGKSLDKESWVKDDENAYILFTSGSTGKPKGVQ
                     ISSNNLDSFSDWISPYLNIDGSEKVIMNQPAYSFDLSVTTIYPGLIHGATLFSISKDV
                     LADYKELFRQFSISDIAVWVSTPSFAGVCITEKEFNSKMLPKLESMIFIGEALSKNLT
                     KELMSRFPNTRIINGYGPTEATVGVSVNDMTQKAIDDEKSLPVGYPMSNCKIKILDED
                     GNELKENEKGEIIIIGPSVSKGYFNNKEKTDEVFFYDEIDGVKWRAYKTGDMGYLLDG
                     NIYYCGRKDFQIKLNGFRIEIEDIENNLRKVHNVKNAVVLPVYKDEKIAYLKGIVELN
                     EKNDLSNIKNGMIIKKELGKYIPSYMIPRNISIISEFPTNINGKIDRKKLMEEI"
     gene            3032..4180
                     /gene="dltB"
                     /locus_tag="KRL67_002258"
     CDS             3032..4180
                     /gene="dltB"
                     /locus_tag="KRL67_002258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltB"
                     /protein_id="HBH1523132.1"
                     /translation="MIFSQYGDYFYLYILLLTSIPAVILGLMGKNIKYYGMLASLFMI
                     FLIVGIDVQLKYLVIFIILEVIIVKGYEYVRRKTKNKYIYWGFLFASMLPIIINKISP
                     VTSFGIIGFIGISYLNFRTIQMVIEIYDGAIKEVKISKMLYFMLFFPTLSSGPIDRSR
                     RFEQDLEKQISRKEYIEEYLLPGIKNIVMGVGYKFVIAFLINTYWVSRIPKDMSFINI
                     LSYMYAYSLYLFFDFAGYSLFAIGTGYIFGIQVPINFDKPFISKDMKEFWTRWHISLS
                     RWFGDYIFSRFVMSSMRKKRFKKRTTAAHVAQMITMITMGFWHGLTWYYVAYGVYQGL
                     ALVLTDIYQRKSKFYKKHKKDKWFERVQIFITFHIVCFGLLIFSGYLA"
     gene            4199..4429
                     /gene="dltC"
                     /locus_tag="KRL67_002259"
     CDS             4199..4429
                     /gene="dltC"
                     /locus_tag="KRL67_002259"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltC"
                     /protein_id="HBH1523133.1"
                     /translation="MQETVIEIFEDVLGTDEIREDLDLNLFETELLDSLAIIEVLLEI
                     ENRLGIELQPTDLERKDMSTVNNLVKFLETRK"
     gene            4646..5431
                     /locus_tag="KRL67_002260"
     CDS             4646..5431
                     /locus_tag="KRL67_002260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HBH1523134.1"
                     /translation="MSLNSIDRKKEILKLLDSKGKVNTKELVKRLEVSSETIRRYLEE
                     LENENKLKKVYGGAIKINYGVIEPEHIKRNSTNLDKKQSIAKYASKFVNDNESIIIDE
                     GTTNLQIVDYIISVNNLKIITNSYPVLSKLISYENQGLFDGEVIFIGGKVNLKHQRTA
                     CPISIDTMKNLYVDKAFISSEGILDSFGISSIDQNKALLSKEYIKNSKESFVLCDSSK
                     IGIASMYKIADLEELDFVICDVEPPNEWKDKLNEINITWIEAK"
     gene            5710..7620
                     /gene="phnW"
                     /locus_tag="KRL67_002261"
     CDS             5710..7620
                     /gene="phnW"
                     /locus_tag="KRL67_002261"
                     /EC_number="2.6.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-aminoethylphosphonate--pyruvate transaminase"
                     /protein_id="HBH1523135.1"
                     /translation="MKKIYGEKIKAVVFDWAGTTVDYGCFAPLNVFIEIFKRRGIDVT
                     MEEARKPMGKLKIDHIREMCEMDRIKNLWSDKFGKVPTEDDVNELYAEFEPMLFETLE
                     DYTTPIPHVVETIEKLRKNGLKIGSTTGYTREMMNIVEPNAAKKGYSPDFLVTPSEVS
                     QGRPYPWMCYKNAEALGVSPMSSMVKVGDTISDVKEGVNAGMWSVAVIKGSSELGLTQ
                     EEVENMDKEELKAKMSIVSKKFKEAGAHFVIETMAELEDILIKIENETIKSDFVPEND
                     YILLTPGPLSTTKSVRASMLKDWCTWDVEYNNLVQDVRRRLVSLATQNTDKYTSVLMQ
                     GSGTFSVEAIIGSTISKDGKLLVIANGAYGKRMKDICNYLDIEFVDCTFKDIEAVDLN
                     VVENLLKENKDITHISMVHCETTTGRLNPIQEVGKLAKKYNKIYIVDAMSSFGGIEID
                     VEDFNIDFLVSSSNKCIQGVPGFGFIIANKEELSKCKGIAKSLSLDVYAQWETMEKNN
                     GKWRFTSPTHVVRAFYQALLELEEEGSVEKRYARYKENQFTIASRLKSLGFDTLVNDN
                     AQSPVITTFLYPKNAKFEFMEFYTYLKDNGFVIYPGKLTDIDTFRIGSIGEVYPTDME
                     RLADVIEKFINR"
     gene            7975..8796
                     /locus_tag="KRL67_002262"
     CDS             7975..8796
                     /locus_tag="KRL67_002262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH1523136.1"
                     /translation="MNEELSSLRFDGVGKCKGGIFDNVNISGTGKIEGSVKCSKFDSS
                     GSAKVFGTTECNEFFTSGLSKVEGNILASKVEISGLLRCTGNINSVEIDTSGIINIEG
                     SIKSDIIHGEGYLKANENIDCENINLSGILECNGFLNCEEVNISLNGISNFNEVGASI
                     INIKKGHGDYCKKFRMLEKKHINFEKVSSNLISLGLLSIRKYTESKLFANIIEADEIS
                     LENSEVKVVRGKNIKIGKGCYVESLEYSESIEIDEDSIVGEIKNISEKVKLKKDK"
     gene            8837..9604
                     /locus_tag="KRL67_002263"
     CDS             8837..9604
                     /locus_tag="KRL67_002263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HBH1523137.1"
                     /translation="MEKGSIKILGEGFISSGEYESIKIVGSSKSKGNVKSNEVKVLGD
                     AKFEGDINIKNLVVNGKTIIIGNLKAERVRVNGQLNIEGDAEIDDLVITAQQKVDGKL
                     KCNNVTIRGQLSVNENMYAQDIKIYGEMRNKSNIECEDIKVYGVIKCDGLLNGENIYV
                     YSGGGSYCKEIGATNIKIGRSKDAFSLIKIINIFSGKFKCNLIEGDTIELEDASIKDI
                     RGKSISLVDNCDVGNIEYSDNLSVSDNSTVKNSIKVG"
     gene            9612..10232
                     /locus_tag="KRL67_002264"
     CDS             9612..10232
                     /locus_tag="KRL67_002264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhbD family protein"
                     /protein_id="HBH1523138.1"
                     /translation="MEELISKKDLLSKTNISYGQLYRWKRKNIIPEEWFIKKSAFTGQ
                     ETFFPRDKILERIELILSMKEETSLDDIANMFMKKDINKEFDIDFILSKEVICNYTKE
                     IFQNLYKKEQSIGKKELLILSIIEKFLVKSTITFEELKLIVNIIEENFMSIYNENGKI
                     YLFRKFGVPFVVGCLDYKKVKFEKDVVKIIEIDLTKEVNDISIKLS"
     gene            complement(10531..11118)
                     /locus_tag="KRL67_002265"
     CDS             complement(10531..11118)
                     /locus_tag="KRL67_002265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubrerythrin family protein"
                     /protein_id="HBH1523139.1"
                     /translation="MNLKDSKTKENLLRAFAGESQARNRYNISASVAKKEGLYIIESL
                     FNYTADQEKSHAKQFFSKLKEFAGENISISGAYPVDLYDKTAQHLKAAQHNELQEWDD
                     VYKNFGKTAREEGFESIAKLFENIAEIEKVHGDRFGRYATDLEEGTLFRRNEDIQWIC
                     THCGHIHIGKEAPKACPVCLHPQGYFLDFKNSLFE"
     gene            complement(11204..11641)
                     /locus_tag="KRL67_002266"
     CDS             complement(11204..11641)
                     /locus_tag="KRL67_002266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HBH1523140.1"
                     /translation="MEDKYIVHFISKTKANMIKFIENKLSKNGLGELIPTHGNILTAL
                     YENNGILTMKEIAAKIGKDKSTVTVLVNKLINLGYLERQKCTNDKRITYIKLTKKALL
                     IEDTFNSISTQVKETAYHNITENEKQEFLRILKKINDNFKNAD"
     gene            11745..12743
                     /locus_tag="KRL67_002267"
     CDS             11745..12743
                     /locus_tag="KRL67_002267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhdH/YhfP family quinone oxidoreductase"
                     /protein_id="HBH1523141.1"
                     /translation="MNKKFKSLYITEEEGNFRRNIVEREVDTLPEGEVLIKVKYSSLN
                     YKDALSANGNKGVTRKYPHTPGIDASGIVESSSCDDFKIGEEVLVTGYDLGMNTSGGF
                     SEYIRVPSSWVVKLPKNLSLKEAMDYGTAGFTAALSVYRFIDSVDKDMGDVLVTGGTG
                     GVAVIATKILIKLGYSVVVSTGKLEEQKEVLLNLGVKDVIHRSEVDDNSGRPLLRPRW
                     AGVIDTVGGNTLATAIKTTNYCGAVTTCGNAGGVDFTSSVYPFILKGITLYGIDSVDC
                     PMNLRLKIWDLLSNEWKLDDLDDMVNVVSLEELNDSIDSIMAGKHVGRTVVDLEKI"
     gene            complement(12863..13789)
                     /locus_tag="KRL67_002268"
     CDS             complement(12863..13789)
                     /locus_tag="KRL67_002268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HBH1523142.1"
                     /translation="MSKENKNVRIAVVQASPVIMDLEKTVEKALGLIKEAGRKGANIV
                     VFPEAFIPAYPRGLSFGFVVGSRTMGGREDWKRYYDNSVPVPSATTDLLGKAAQEAGV
                     YLSMGITERDGNDINCTLYCTNLFFSPEGKLIGKHRKLKPTGTERCIWGEGDGSTLTV
                     VDTPYGKMGSLICWENYMPLARTALYAKGVKLYIAPTADSREEWQATMKHIALEGRCF
                     VIGCNQYVEKNMYPTDLNYYKELDAEPEIMCPGGSCIVDPFGKYVAGPIFNKEEMLIA
                     DLDLEKIVLSRLDFDSEGHYSRPDVFELIVHE"
     gene            14175..15779
                     /locus_tag="KRL67_002269"
     CDS             14175..15779
                     /locus_tag="KRL67_002269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HBH1523143.1"
                     /translation="MKEKIYYNGNIITMEDSICGDAILIKDKIIKKIGTKEEVFALKN
                     KDTEIIDLQGKTLMPSFIDSHSHLIAFATTLKLVPLEDATSFKDIVKKIQDFKESNNI
                     KKGDWIIGFSYDNNFLEENKHPDKSVLDSASSENPILISHASGHMGVANTLGLEQLGV
                     TNETQDPEGGHIGRAEGSKEPNGYLEENAFFNVASKIKQPSSNEIFNSIEKAQNIYLR
                     YGITTAQEGLMEENQFNILKAMANQNKLKMDVVGYVNLKKSKSVADNNREFIKKYVNR
                     FKIGGYKIFLDGSPQGKTAWLSRPYENSDDGYCGYPIYKDEEVEKFIDISLKEKMQLL
                     THCNGDAAADQLIDAFEKVLNLKEQSSENNIRPVMIHAQTVRADQIDDMKVINMIPSY
                     FVAHTYYWGDIHIKNLGEDRAFKISPLKTTIEKGLIYTLHQDTPVIAPNMLETVWCAV
                     NRITKKGIQIGENEKISPLDALKGVTINAAYQYFEEDKKGSIKEGKLANLIILDENPL
                     TIDPMKIKDIKVLQTIREGEVLYSLK"
BASE COUNT         6428 a         1721 c         2777 g         5324 t
ORIGIN      
        1 caagtgttaa aatgtcaaat tataagtatg aaaaagtgtt aaaaagaaat ttatttttgg
       61 tgcttttttt tctttttttt acaattttgt aacagtatca aaaaaagttt tgacgatttt
      121 attacaattt tgtagttgaa tatacatatc aaaactaata tgattaataa taacataaat
      181 ttgaatattt ttagaggaga gaaaatatga gaaaattgat atactttatt acacctttta
      241 taataggcgt agtattttta tttgggttag acaaatttct tgattctaaa actgatgaac
      301 ttttgaggga gaaaaatcta cttccaatta tggatgatac tttaagtgat ataaaagata
      361 aaggggttac agcaaataac cattttttaa gagaaaaaga tattatgatt ttggggtcat
      421 cagaacttag taattcaaca aaacaacatc caaagtacta ttttaataca aataggagta
      481 aaaataaggt gtttgcaatt gggagagcct atactcagac attacaagat gctgcaatat
      541 taggaagtat gaatcctaat attgataata agaaagttgt cttgttaatt tctatgcaat
      601 ggtttatgga aaaagacgga gtcacaagtc accattatca gtctagattt tcaccaatac
      661 aattttatcg atttttagat aatccaaaaa tatctaaaca aaataaaatt gaatatgcaa
      721 aaaaatcaag taagctatta tggggaagtg acgagtataa agcagaagca ttatatgcga
      781 agctatatga gcctaaaaca ttgcctgaaa aagctgaaaa ggtgctttta gagccttatt
      841 ttcaaggaag aaagtactgt atagctctta aagaaaaggg tattctttat aaacgattaa
      901 ttaagctaga taaaaagaga gcaacaaaaa gaaaaagacc aataaattgg tcatatgaga
      961 gaaagaaagc cattgaagat gctaaaaaaa gagttggtaa aaatccactg aatattgata
     1021 agtattatta taaagagcat tttaaagatg gtatagagca atacaaagga agagacaaag
     1081 atgtaaattt attaacatct aaggagtttg aaaattataa actgatgcta aatgtatgta
     1141 ctgacttagg tattaagcca gttgttgtat taatcccaag tatggataaa ttctataatt
     1201 tgactggtat ttctgaaaaa gaaagaaatg agtactacga taaagcacaa aaaatagcag
     1261 aaagcaaagg ctttgaagtt ctaaatctta aagataaagg gtcagataag tattatttaa
     1321 gggatgtaat gcatcttggt acgaaaggat gggtagatgt ctgtgagaga ttatttaaaa
     1381 tatttaaaga acaataaaga tttgttaact agtatttgtt taactatggt tataatattg
     1441 gcgataggag ttctaacatt tactccattt aaagaattgc catttattta taacggattt
     1501 taaaggagca ttcttatatg aaaattattg aaggaataaa aaaatattca aatacagata
     1561 gaacagcact aatgtgtaat ggagataaac tttcttataa agatttaaat gaatattcag
     1621 atgctatatc agtatttcta aaggatgtat acaaagaaga agatactcct atagtaattt
     1681 atggaaataa agaaaatatg ataatggcat gtatgatagg agctttaaaa tcaggtagag
     1741 cgtatgtgcc tcttgatatt agttttccaa ttgatagagt ttttgaagtc actaaagaga
     1801 taaaaccaaa agtaatattt aactttagtg atgaaagaga ttttggagat atcgatgtaa
     1861 ttgatatgga taaattaaag gatataataa atgaatatca gggaaaatca ttagataaag
     1921 aaagctgggt aaaagatgac gaaaatgcat atatattatt tacatcagga agtacaggaa
     1981 aaccaaaagg agttcaaata agttcaaaca atttagatag cttttcagat tggataagcc
     2041 catatctaaa catagatgga agtgaaaaag taataatgaa ccaaccagca tattcttttg
     2101 acctttcagt tacaacaata taccctggtc ttatccatgg agctacatta ttttcaatat
     2161 caaaagatgt attagcagac tataaagaac tatttagaca atttagtatc tcagatatag
     2221 ctgtatgggt gtcaactcct tcttttgcag gagtatgtat aactgaaaaa gagtttaata
     2281 gtaaaatgct tcctaagctt gagtcaatga tattcatagg agaagcactt tcaaaaaatc
     2341 ttactaaaga acttatgagt agatttccaa acacaagaat tatcaatgga tatggaccaa
     2401 ccgaggctac tgttggtgta agtgtaaatg atatgactca aaaagctata gatgatgaaa
     2461 aaagtcttcc agttggatat cctatgtcaa attgtaaaat aaaaatactt gatgaagatg
     2521 gaaatgaact taaagaaaat gaaaagggtg agattataat aattggacca tcagtttcta
     2581 aaggttactt caataataaa gaaaagactg atgaagtatt tttctatgat gaaatagatg
     2641 gtgtaaagtg gagagcttat aaaactggag atatgggata tcttttagat ggaaacatat
     2701 attattgtgg aagaaaagat tttcagatta aattaaatgg atttagaatt gaaatagaag
     2761 atatagaaaa taatttgaga aaagtacata atgtaaaaaa tgcagtagtt ttaccagtat
     2821 ataaggacga aaaaattgct tatttaaaag gtattgttga gttaaatgaa aagaatgatt
     2881 taagtaatat taaaaatggg atgataataa aaaaggaact tggaaagtat ataccttcat
     2941 atatgattcc aagaaatatt tcaataatct ctgagtttcc aacaaatatc aatggtaaaa
     3001 tagacagaaa gaaacttatg gaggaaattt aatgattttt tcacagtatg gagattattt
     3061 ttatctttat atattattat taacatcaat accagcagta attttaggcc taatgggcaa
     3121 gaatataaag tattatggta tgttggcaag tttgtttatg atattcttaa tagtaggaat
     3181 tgatgtccaa ctaaagtact tggtaatatt tattattctt gaagtaataa tagttaaagg
     3241 atatgaatat gtaagaagaa aaactaaaaa taaatacata tattggggat tcttgtttgc
     3301 atcaatgctc ccaataataa taaataaaat atctccagtt acttcttttg gaataattgg
     3361 atttattgga atatcatact taaatttcag aactatacaa atggttattg aaatttatga
     3421 tggagctata aaagaagtta aaatttcaaa gatgctttat tttatgttat tttttccaac
     3481 tttaagttct ggacctattg ataggtcaag aagatttgaa caggacttgg aaaaacaaat
     3541 ctctagaaaa gagtatatag aagaatactt actgccagga ataaaaaata ttgttatggg
     3601 agtaggttat aagtttgtta tagcattttt gataaataca tattgggtat caagaatacc
     3661 taaagatatg agttttataa atattttaag ttatatgtac gcatatagtt tatacctgtt
     3721 ctttgatttt gcaggatata gtttatttgc tataggtaca gggtacatat ttggaataca
     3781 agtacctata aactttgaca agccatttat tagtaaagat atgaaagagt tctggacaag
     3841 atggcatata agtctctcaa gatggtttgg agattatatt ttttctagat ttgttatgtc
     3901 atcaatgaga aagaaaagat ttaagaaaag gactactgct gctcatgtgg ctcagatgat
     3961 aacaatgata acaatgggat tttggcatgg tctaacttgg tattatgttg cttatggtgt
     4021 ataccaagga ttggcacttg tgttaactga tatatatcag cgaaaatcta aattttataa
     4081 aaaacataaa aaagataaat ggttcgagag agtgcagatt ttcattacat tccatattgt
     4141 gtgttttgga ttgctgatat tctcaggtta tttagcttaa ttttaaggag gaaaatttat
     4201 gcaagaaaca gttatagaaa tatttgaaga tgtattaggg actgatgaga taagagaaga
     4261 tttagacctt aatttatttg agacagaact tttagactca ttagctataa ttgaagtttt
     4321 acttgaaatt gaaaatagat tagggataga gcttcaacca actgatttag agagaaaaga
     4381 tatgtctact gtaaataatt tagtaaaatt cttggagaca agaaaataga ttagtagtta
     4441 gactttaaat attaatttat aaaacaaaat tttacaaagt ccatctgata ctaatatttg
     4501 atggactttt ttaatgataa tacaaagtct tttgaaaata actttaatta agtaaaaaat
     4561 ttaatatata aatatgattt cataaatctt aggtatcatt gtaatattat aggagaaaaa
     4621 tatattatat ttaggaggaa aacggatgtc tttaaattcg atagatagaa aaaaagaaat
     4681 acttaaatta ttagattcaa aagggaaggt aaatactaaa gagttagtaa aaagattaga
     4741 agtatcatca gaaacaataa gaagatattt ggaagaatta gaaaatgaaa ataaattaaa
     4801 aaaggtatat ggtggtgcta taaaaataaa ttatggagtg attgaaccag aacatataaa
     4861 gagaaacagt actaacctag ataaaaagca aagtattgct aaatatgcat ctaagtttgt
     4921 aaatgacaat gagtcaatca tcatagatga aggtactacc aatttgcaga ttgtagatta
     4981 tataatatct gtaaacaatt taaaaattat tacaaactct tatccagttc tatcaaaatt
     5041 gatttcttac gagaatcaag gcttatttga tggagaagtt atatttattg gtggaaaagt
     5101 aaatttaaaa caccaaagga cagcatgtcc aatatcaata gacactatga aaaacttata
     5161 cgtagataaa gcttttatat ctagtgaagg tatacttgat agttttggaa ttagtagtat
     5221 agaccaaaat aaggcattgt tgtcaaaaga atatataaaa aattcaaagg aaagttttgt
     5281 attatgtgat agttctaaaa taggaattgc tagtatgtat aagatagcag atttagagga
     5341 attagatttt gttatttgtg atgtagaacc tccgaatgaa tggaaggata agttaaatga
     5401 gataaatata acatggatag aagcaaaata aacccttagt tttatataat acaactatgc
     5461 ttaatataac taaattcata aaatatagtt agttcataga ttaagattag aaagtaaatt
     5521 ttagattgta acttaggatt ttatggaaat acgatacaaa ataaaaacaa ctaaatacaa
     5581 ctaaaaacag aaatttaata tatatttaac ctaaatatga tgtataatta acatatgaaa
     5641 gttatacttt aaaagtaaaa acaattaaaa acacaaaaat tctaaaaaga atctatggag
     5701 gctataaata tgaaaaagat atatggagaa aaaataaaag cagtagtatt cgattgggct
     5761 ggaacaacag tagattatgg atgttttgca cctcttaatg tatttataga gatatttaaa
     5821 agaagaggta tagatgtaac aatggaagaa gcaagaaaac ctatgggaaa attaaaaata
     5881 gaccatataa gagaaatgtg tgaaatggat agaataaaaa atctttggtc agataagttt
     5941 ggtaaagtgc caactgaaga cgatgtaaat gaactttatg ctgaatttga gccaatgtta
     6001 tttgagacat tagaagatta tacgactcca attcctcatg ttgttgaaac tattgaaaaa
     6061 ttgagaaaaa atggattaaa aataggttct acaacgggtt atacaagaga gatgatgaac
     6121 atagttgaac caaatgcagc aaagaaagga tactcaccag attttcttgt aacaccatca
     6181 gaagtatctc aaggaagacc atatccttgg atgtgctata aaaatgctga agctttaggg
     6241 gtgtctccta tgagttctat ggtaaaagta ggagatacaa taagtgatgt aaaagaaggc
     6301 gtaaatgcag gaatgtggag tgttgctgta ataaaaggtt caagtgagtt agggcttaca
     6361 caagaagaag tagagaatat ggataaggaa gaattaaaag ctaagatgag tatagtttca
     6421 aagaaattta aagaggcagg tgcacatttt gtaatagaaa ctatggctga gttagaagat
     6481 atactaataa aaatagagaa cgaaactatt aaatcagatt ttgtaccaga aaatgattac
     6541 atattactta caccaggacc attatctact actaaatcag taagagcatc tatgttaaaa
     6601 gattggtgta cttgggatgt tgaatataat aacctagtac aagatgttag aagaagatta
     6661 gtatctctag caacccaaaa tacagataaa tacacaagtg tattaatgca aggaagtggg
     6721 actttttcag tagaagctat aataggaagt actatttcaa aagatggtaa acttcttgta
     6781 attgcaaatg gagcatatgg aaagagaatg aaagatatat gtaattatct tgacatagaa
     6841 tttgttgact gcacatttaa agatatagaa gcagtggatt taaatgtagt agaaaattta
     6901 ttaaaagaaa acaaagatat aacacatata tcaatggttc actgtgaaac tactacaggt
     6961 agattaaacc ctatacaaga ggtagggaaa cttgcaaaaa aatataacaa aatttatata
     7021 gttgatgcta tgagtagttt tgggggaatt gagattgatg ttgaagattt taatatagat
     7081 tttttagtta gtagttctaa caaatgtata caaggagtac ctggatttgg ttttataata
     7141 gctaataaag aggaattatc taaatgtaaa ggtatagcta aatcattatc tcttgatgtg
     7201 tatgcgcaat gggagactat ggaaaaaaat aatggcaaat ggagatttac ttcacctaca
     7261 catgttgtaa gagcttttta tcaagcctta ttagaattag aagaagaagg ttctgttgaa
     7321 aaaagatatg ctagatataa agaaaatcag tttacaatag cttcaagatt aaaatcttta
     7381 ggctttgata ctttggtgaa tgataatgct caatcacctg taataacaac tttcttatat
     7441 cctaaaaatg caaagtttga atttatggaa ttttatactt atttaaaaga taatggattt
     7501 gttatctatc caggtaaatt gactgatata gatacattta gaataggtag cataggtgaa
     7561 gtttatccaa ctgatatgga gagactagct gatgtaatag aaaaatttat taatagatag
     7621 aagaagatag tgtcttgtag cttacagaat agtgcagaaa gcccactcct ttaggtgtga
     7681 gatagtacaa acagtgtata agaagtattg acaataggat ttaaagactt atattttaga
     7741 agtttataca atgaatatat aaagattgag taatagggat gtattttact taaaccacaa
     7801 tctaagaaat tagatttata gtttaaataa agcattttcc ctattatttt tatgcctatt
     7861 tatagaaaat aaataatagt tgttttatta agtattatta tcatacttga caacgtaaca
     7921 atgttttgtt atattatgtt taaagataga atacggtaaa gaaaggatat cattatgaat
     7981 gaagaattaa gttcactaag atttgatggt gttggaaaat gtaaaggtgg aatttttgat
     8041 aatgttaata tatcaggaac aggaaaaata gaaggaagtg taaaatgtag taaatttgat
     8101 tcttctggtt cagcaaaagt ttttgggaca acagaatgta atgaattttt tacatctggt
     8161 ttaagtaaag tagagggaaa catactggca tcaaaagttg agatatcggg gcttcttaga
     8221 tgtacaggaa atattaattc tgttgaaatt gatactagtg gaattatcaa tatagaagga
     8281 agtataaaat cggatattat tcatggagaa ggatatttaa aagcaaatga gaatatagat
     8341 tgtgaaaata tcaatttaag tggaatcttg gagtgtaatg gatttttgaa ttgtgaagaa
     8401 gttaatatta gtttaaatgg aatttctaat tttaatgaag ttggagcaag tattataaat
     8461 ataaaaaagg ggcatggaga ttattgcaaa aaatttcgta tgctagaaaa aaagcatatc
     8521 aactttgaaa aagtgtcttc aaatttaata agtttgggtc tgttatcaat tagaaagtat
     8581 acagaaagta aattgtttgc aaatattata gaagcagatg aaataagctt agaaaatagt
     8641 gaagttaaag ttgtaagagg aaaaaatatt aaaataggca agggatgtta tgtagaaagt
     8701 ttggagtaca gtgaaagtat agaaattgat gaagattcaa tagttggtga aataaagaat
     8761 ataagtgaaa aagtaaaatt aaagaaggat aagtaataag taggtttaaa aataaaattt
     8821 aaaatgaggt aataatatgg aaaaagggtc tataaaaata cttggagaag gctttataag
     8881 tagtggtgag tatgaaagta taaaaatcgt gggaagttct aaatcaaaag gaaatgttaa
     8941 aagtaacgaa gtaaaagtcc ttggagatgc aaaatttgaa ggtgatatca atataaaaaa
     9001 tttggttgtt aacggaaaaa ctattataat aggcaatctt aaagcagagc gtgtaagagt
     9061 gaatggtcaa cttaacatag agggagatgc agaaatagat gacttagtta taactgctca
     9121 acagaaagtg gatggcaaac ttaaatgtaa taatgttaca ataagaggac aattaagtgt
     9181 aaatgaaaat atgtatgcac aagatataaa aatctatggc gaaatgagaa ataaatcaaa
     9241 tatagaatgt gaagatataa aggtatatgg agtgataaaa tgtgatggtc ttttaaatgg
     9301 agaaaatata tatgtttatt caggaggagg ttcttattgt aaggagatag gggcaactaa
     9361 tataaaaata ggaagaagca aagatgcttt ttcattaatt aaaattatta atattttttc
     9421 aggaaagttt aaatgtaatt tgatagaggg agatactatt gaattagaag atgcatcaat
     9481 aaaagatata cgaggtaaaa gcatatcgct agtagataat tgtgatgttg gaaatattga
     9541 atacagtgat aatttatcgg tttcagataa ttctactgta aaaaacagta taaaggtggg
     9601 ctagaagagt tatggaagag ttaatatcta agaaagattt attgagcaaa actaatatat
     9661 cttatgggca attatataga tggaaaagaa aaaacataat accagaggaa tggtttataa
     9721 aaaaatcagc ttttacaggt caagagactt tctttccaag agacaaaata ctagaaagaa
     9781 tagaattaat attgtctatg aaagaagaga cttcccttga tgatattgca aatatgttta
     9841 tgaaaaaaga tattaataaa gaatttgaca ttgattttat tctcagtaaa gaagttatat
     9901 gtaattacac taaagaaata tttcaaaatt tatataagaa agagcaaagc ataggtaaaa
     9961 aagaactgct tatattgagt ataatagaga aatttttagt taaatctaca ataacttttg
    10021 aagagttaaa attgatagtg aatataatag aagaaaattt tatgtcaata tacaatgaaa
    10081 atggaaagat ttatttgttt agaaagtttg gagttccatt cgtagtagga tgtctggatt
    10141 ataaaaaagt aaaatttgaa aaagatgtag ttaagattat tgagattgac ttgacaaaag
    10201 aagtaaatga tataagtata aagttatcat agtgcattat cttatataat gtattataat
    10261 aatctactta aaatacactt tatttaaata tttagtttaa agatgagatt ttatgtgagt
    10321 atgtaaaagt taaagataga tttaaataga ttatagggat tagtaaacaa taaaatgaac
    10381 catgatgaac tactataaat tgtatttaaa ctacattttc tacttatagt aatatcaagg
    10441 gttcatttta ttgttattaa gataatacag tatctataaa aatacttata taaatatagt
    10501 aaattattta aatacaatga ttaccttagt ttattcaaat aatgaatttt taaaatctaa
    10561 gaaatatcct tgtggatgaa gacatacagg acaagctttt ggagcttctt tacctatatg
    10621 tatatggcca caatgtgtac atatccattg tatatcttca tttcttctga ataaagtacc
    10681 ttcttctaaa tcagtagcat atctaccaaa tctatcacca tgaacttttt ctatttcagc
    10741 tatattttca aataacttag ctatagattc aaatccttct tcacgtgctg tttttccaaa
    10801 gtttttatat acatcatccc actcttgaag ctcattatgt tgtgcagctt taaggtgttg
    10861 tgctgtttta tcatataaat ctacagggta tgctcctgat atacttatat tttcaccagc
    10921 aaattctttt aatttagaaa agaattgttt tgcatgtgat ttttcttggt cagcagtgta
    10981 attaaataaa gactctatta tatataatcc ttcttttttt gccacagatg cagaaatatt
    11041 gtatctattt ctagcttgac tctcacctgc aaaagctctt aataagtttt ctttagtttt
    11101 actatctttt aaattcatat tctacctctc tatcacaata aatttttaca atatttgatt
    11161 ctttttattt attattccaa taggagatat aattatgctc aatttaatca gcatttttaa
    11221 aattatcatt aatttttttt aaaattctaa gaaattcttg tttttcattt tctgtaatat
    11281 tatggtatgc tgtctcttta acttgtgttg agatgctatt aaatgtatct tctataagta
    11341 atgccttttt tgtaagcttt atatacgtga tacgtttgtc atttgtacac ttttgtcttt
    11401 ctaaatatcc tagatttatg agtttattaa ccaacactgt aacagtggat ttatcttttc
    11461 ctattttagc tgcaatttct ttcattgtaa gtattccatt attttcataa agtgcagtta
    11521 aaatatttcc atgagtaggt attaactcac ctaaaccatt tttactgagt ttattttcaa
    11581 tgaatttaat catattagcc tttgttttac ttatgaaatg tacaatatat ttatcttcca
    11641 taaattattc tccaatcttt aatctattta aattagtttg acataaaact atttataaac
    11701 tataattagt ttaacataaa actaaatgta ttgggaggaa atttatgaat aaaaaattta
    11761 aatcactata tattacagaa gaagaaggca attttagaag aaatatagtt gaaagagaag
    11821 tagatacatt gccagaagga gaagttctaa taaaagtaaa atattcttct ctaaattaca
    11881 aggatgcact ttctgcaaat ggtaataagg gtgttacgag aaagtatcct catacaccag
    11941 ggattgatgc atctggtata gtagaatcta gtagttgtga tgattttaaa attggagaag
    12001 aagttttagt aacaggttat gacttgggaa tgaatacttc tggtggtttt tcagaataca
    12061 taagagttcc ttctagttgg gtagttaaac ttcctaaaaa tctttcacta aaagaagcaa
    12121 tggattatgg tacagcagga tttacagcag ctttatctgt gtacagattt atagattctg
    12181 tagacaagga tatgggagat gtattagtca ctggtggtac tggaggagtt gctgtaattg
    12241 caactaaaat attgattaaa ttaggttatt cagttgtagt ttcaacagga aaattagaag
    12301 aacaaaaaga agtattacta aacttaggtg ttaaagatgt tatacatagg tcagaagtgg
    12361 atgataattc aggaagacct ctgcttagac caagatgggc tggagtaata gatacagtag
    12421 gaggaaatac tctagcaaca gcaataaaga caactaatta ttgtggagca gtaacaactt
    12481 gtggtaatgc aggaggagtt gattttacaa gttcagtata tccatttatc ttaaagggaa
    12541 ttactttata tggtatagat tctgtggatt gcccaatgaa cttaagatta aaaatatggg
    12601 atttattatc aaatgaatgg aaattagatg atttagatga tatggtaaat gtagtatcat
    12661 tggaagagtt aaatgatagt atagattcaa taatggcagg aaaacatgtt ggaagaacag
    12721 tagttgattt agaaaaaata taggtgaata aatttataat taggattcta ggttttaagc
    12781 aaaaatgaga tacttgaaat ttacttatga gtatctcatt tttttatgta aaaaaattta
    12841 tgtagatgtc tgtgtaaaaa aattattcat gaacaattaa ttcaaataca tctggtcttg
    12901 aatagtgacc ttctgaatca aaatctagtc tactaagtac tattttttct aggtctaaat
    12961 cagctattag catttcttct ttattgaaaa taggacctgc tacatatttt ccaaaagggt
    13021 caactataca acttcctcct ggacacataa tctctggttc tgcatctagc tccttatagt
    13081 aattaaggtc agttggatac atattttttt caacatattg attgcatcca attacaaaac
    13141 atcttccttc taaagcaata tgcttcatag ttgcttgcca ctcttctctt gaatctgcag
    13201 taggggctat gtaaagtttt acacccttag cataaagagc tgttctagct aatggcatat
    13261 aattttccca acaaatcaac gaacccattt ttccatatgg agtatcaact actgtaagag
    13321 tacttccatc tccttctccc caaatacatc tttctgttcc tgtaggttta agttttctgt
    13381 gtttaccaat tagcttacct tctggagaga aaaataagtt tgtgcaataa agggtacaat
    13441 ttatatcatt accatctctt tctgttatac ccattgatag atatactcct gcttcttgtg
    13501 ctgcttttcc taataaatct gttgtagcac taggaactgg cacagaatta tcatagtatc
    13561 ttttccaatc ttctctaccc cccattgttc tgcttccaac aacaaatcca aatgaaagac
    13621 ctcttggata tgcaggtatg aatgcttcag gaaaaactac gatatttgcc ccttttctac
    13681 cagcttcttt aattaatcct aatgcttttt caacagtttt ttctaaatcc attatgactg
    13741 gtgacgcctg gacaacagca attcttacat ttttattttc cttgctcata aaaaacctcc
    13801 taaaaaatat ttaaattttt tgcattgaat agattacatg agatgttatt tgagaattaa
    13861 gatatttata ttgtgaataa taatgtaact atatatttaa aataatacat catgatttaa
    13921 aaaattacaa ttgtacagat ataattgagt ttagatattt aaatttaaca acttataata
    13981 ttttttcaat tagtataaag ctgattaatt tgtcttattt acaatgaata gtatttactt
    14041 cttggcaaac aataattaaa aataaaactt ttatataatt aaactctaaa gaaaaagttt
    14101 ataaagaaga taatataatt aagtagaaaa taatgaaaaa aatttattaa agtagacaac
    14161 gtaaggagaa ttttatgaaa gaaaaaattt attacaatgg aaatatcatc acaatggaag
    14221 attcaatatg tggagatgct attcttataa aagacaaaat aataaaaaag ataggaacaa
    14281 aagaagaagt atttgctttg aaaaataaag atacagaaat tatagattta caaggtaaga
    14341 cattgatgcc atcttttata gattcacata gtcacttgat agcttttgct actactctaa
    14401 agctagttcc tttagaagat gccactagtt ttaaagacat agtaaaaaaa atacaggatt
    14461 tcaaagagtc gaataatata aaaaaaggag actggataat tggattttca tatgataata
    14521 attttttaga agaaaataag catccagata agagtgtact cgatagtgca tcttcagaaa
    14581 atccaatact aatttcacat gcttctggtc atatgggagt tgcaaataca ttggggttag
    14641 aacagcttgg tgttactaat gaaacacagg acccagaagg tggacatata ggtagagcag
    14701 aaggaagtaa agaaccaaat ggatatttgg aagaaaatgc attttttaat gtagcgtcta
    14761 aaataaaaca accatcatca aatgagattt ttaattcaat tgaaaaagca cagaacatat
    14821 atctaaggta tggtattaca acagctcaag aaggtttaat ggaagaaaat caatttaaca
    14881 ttttaaaagc aatggcaaat caaaataaat taaaaatgga tgttgtagga tatgtaaatt
    14941 tgaaaaaaag taagtctgta gcagataata atagggagtt tataaaaaaa tatgtgaata
    15001 gatttaaaat aggaggctat aagattttct tagatggttc tccacaaggt aagacagcat
    15061 ggttgagtag accatatgaa aattcagatg atggttattg tgggtatcct atatacaaag
    15121 atgaagaagt agaaaagttt atagatattt cactaaaaga aaagatgcaa ctcttaactc
    15181 attgtaatgg agatgcagca gcagaccagt tgatagatgc gtttgagaaa gttttaaact
    15241 taaaagagca aagtagtgaa aataatataa gaccagttat gatacatgct caaacagtaa
    15301 gagcagacca gattgatgat atgaaagtaa taaatatgat accttcatac tttgtagctc
    15361 acacatatta ttggggtgat attcatatca aaaaccttgg agaagataga gcgtttaaga
    15421 ttagcccact taaaacaact atagaaaagg gattaattta cactcttcat caggatacac
    15481 cagtaatagc acctaatatg ctggaaacag tatggtgcgc tgtcaataga attacaaaaa
    15541 aaggtataca aataggagaa aatgaaaaga taagcccatt agatgcttta aagggtgtta
    15601 ctataaatgc agcatatcaa tattttgaag aagataaaaa aggttcaatt aaagaaggta
    15661 aattagctaa tttgataata ttagatgaaa acccacttac aatcgaccct atgaaaataa
    15721 aagatattaa agtattgcag actataagag aaggagaagt attgtattct ttgaagtaga
    15781 tgaaagttag atgttttata atctataaaa aacagcttta tataataaat taggtaggat
    15841 taaataaggt tacagttaaa tcaacaatta tattttttat taaattttaa ttattgtatt
    15901 gaagatttat aagtataatt ttattgattc taccttttta aataactgtc gatttattaa
    15961 agttatctat gtactatatt aaacaattaa gtagttatta attacactta ttagtttatt
    16021 aaaaaactat gtatattact ttgcagtaag actttggagc taaatctata atagtaaatt
    16081 taaatttatt aaatagatta ccttataaca ttccatataa aatatattgt gaatctgaga
    16141 aaattactat ttttctttga gtatttttta taagcagttc taaaaggttc aatttcatac
    16201 ctatacttga atcattcatt aaaataattc taatctaaaa aataatctta
//
LOCUS       DAEPXF010000083        16193 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain HB27
            SAMN13376389-rid12479423.denovo.087, whole genome shotgun sequence.
ACCESSION   DAEPXF010000083 DAEPXF010000000
VERSION     DAEPXF010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13376389
            Sequence Read Archive: SRR10518432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 16193)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16193)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001069624.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001069624.1
            Genome Coverage       :: 315x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/15/2021 01:54:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,165
            CDSs (total)                      :: 4,076
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 89
            rRNAs                             :: 10, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 10 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 19 of 69
            Pseudo Genes (incomplete)         :: 44 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 10 of 69
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16193
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13376389-rid12479423.denovo.087"
                     /strain="HB27"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="China: Hebei"
                     /collection_date="2017"
     gene            34..906
                     /locus_tag="KRL67_002270"
     CDS             34..906
                     /locus_tag="KRL67_002270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HBH1523144.1"
                     /translation="MNILQNENLIIESSSFGAELTKIFSKKYDKNILWDGNKKYWGRQ
                     SPILFPIVGKLFDNETIIEENLYSMTQHGFARDMDFEIVDKGDAFVSYKLTDNESTLK
                     KYPYSFELVINYTLNDNSIGVEWIVKNTDSKDIFFSIGGHPAFNLPFYEQNDFSKHYL
                     EFKSRNDVEKINLNGSFTDDIISIGKLKNLQLNPEVFKNDALIYTNIDEVSICNNDGS
                     KYVTISMEDFPLVGIWTPYYNETNSTAPFLCIEPWYGLADSINSNKIYKDKKFINKLS
                     KGKVFTASYNINIH"
     gene            complement(1103..2290)
                     /locus_tag="KRL67_002271"
     CDS             complement(1103..2290)
                     /locus_tag="KRL67_002271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HBH1523145.1"
                     /translation="MKYNFDKVLNRKEGNCRKWSNHVIKEKFGLNEDAIPMDLADIDF
                     ECAPAIKESILKRASVGDYSYTFIGDDFYDAIINWNKRRFDVYIEKDWIKLTFGTVST
                     LHYIVQAYTKEGEGVLINTPAYDPFAEAIVNNNRKLCCSSLKLDNNRYYLDLEDMENQ
                     MKYENIRLLIFCSPQNPSGRIWTKEELYQVSELCLKYNVILVSDEIHRDIIFKDYRFV
                     SLWNAHPEIYKNSIICVSPNKGFNLGGLKTSYVLIRDLKIRETLLERLKSNSITSPNV
                     FAIPAIVAAYNESEEWLDSMTSYVEENFEIVYDFFETHIPKANVMKSDSSFLAWIDVR
                     EVFRNEEESKEFFRHANLTMVVGSYFVKDGDGFIRINVGCPRETLKEALNRIKKTYIG
                     MYC"
     gene            complement(2295..3365)
                     /locus_tag="KRL67_002272"
     CDS             complement(2295..3365)
                     /locus_tag="KRL67_002272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HBH1523146.1"
                     /translation="MNNTMERLVSLICKRGVDALLIKAKNTKRYIGALSGSGVYVLVT
                     KDKNYQIIDGRYIDEADKKTTGFIKRVVSQGSYIPDIIELLRELHIKKLAIENQAMSI
                     QEYLSLKNAGFEINLITNELWKLRAIKSKEEIELIKKACEITDEVFSEVVCEIKKDMT
                     ELEVSALIQYHALRKGASGMAFETIVVSGERGAMPHGRPTNKKLKVNEAITIDFGVVY
                     QGYQSDMTRTISIGKPPKIIKEIYDVVLEAQLSAIESIKEGVNARDIDKVAREIIDKY
                     GFGEYFNHGLGHGIGLGDGEVPTLNPNSEDILTEGMVMSCEPGIYIPNVGGVRVEDDI
                     VIINGKGVSLNKTSKEFIILGE"
     gene            complement(3375..4697)
                     /locus_tag="KRL67_002273"
     CDS             complement(3375..4697)
                     /locus_tag="KRL67_002273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBH1523147.1"
                     /translation="MFSKFEAFMNRVFMPLAHKVDNQRHLGSIKAGMVAMTPFTILGS
                     IFAILPALPNMLGENNPISKFILNNSAILDLPVSLSIGLIGLYACMCIAFNLGNYYKL
                     YIPGCVALSTFSFLFLVASFTPDGALEIGNLGAKGLFTGMIAGLGSVELYNFCNKKNI
                     TIKMPENVPDFVSRSFELIPTTIIVCGTFIVARFVSLKVFGELPPQILTQFLSPLVGS
                     MDNPWSVLALNFAICTIFFFGIHSSVFSPITRPIMITFIAENIAAMQAGETIPHFFTA
                     GASSAFFGFTGCGISIGCVIACMLSKNASYKKIGRVSLFPALFGINEPILFGAPIILN
                     PIMFIPHVFGGAIIGTLPMFFMHWGWLAKPVFDPPYVGVFLEGFLTNGDWHSILANAL
                     QLVLAVLLYWPFFKIMERQQNNKKQREETKNIFSKEEEDLLDDLGLDF"
     gene            complement(4729..5064)
                     /locus_tag="KRL67_002274"
     CDS             complement(4729..5064)
                     /locus_tag="KRL67_002274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBH1523148.1"
                     /translation="MKILLCCAGGFSTNMLMQNMKKVVKESAKLNEKDFEFTAIPADS
                     IESEVDDWDIVLIGPQIAHKIDFLKGILEPKNKPFAVIDKDVYGKMDGATVMKLALIT
                     YKKHQLAQG"
     gene            complement(5161..5466)
                     /locus_tag="KRL67_002275"
     CDS             complement(5161..5466)
                     /locus_tag="KRL67_002275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HBH1523149.1"
                     /translation="MSTEVQIMELISTAGESKSKAFEALKKVKSKDFKGAKLLIEEAR
                     KIDVEAHRIQTELITQDLNEDVESPAMSLLMVHAQDHYMTSQLAKDLIEALIYCLDK"
     gene            complement(5481..6524)
                     /gene="ypdE"
                     /locus_tag="KRL67_002276"
     CDS             complement(5481..6524)
                     /gene="ypdE"
                     /locus_tag="KRL67_002276"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HBH1523150.1"
                     /translation="MNIEFFKRLSEADGIASNEEEVRHVLLEELKAYSDKVICDGLGS
                     IIFSKIKDESAPNIMICAHMDEVGFMVRSIDKLGMIHLITIGGVKPLAQFTQKIRITT
                     REGKKIPAIINATYNNGKAENIYADIGAYTEEDVYSLGINVGDMVTYTTSFEEFTLPD
                     RLVGKAFDDRIGCFVMGEVLKELKKENINCNIHFAATSSEEVGIRGAKTSTQLINPDI
                     VFVIDVACAKNEFVRDYTNQKQIDRGVMLMHRDRTLVPSRKMIDYVMKIADENNIPLQ
                     HDMFESGGTDGGEAHLVNEGKPCVVTCVPVRYGHCAFSIVSNKDLKNIIKLYTQLILN
                     FDEEKYKHIRNFI"
     gene            complement(6527..8428)
                     /locus_tag="KRL67_002277"
     CDS             complement(6527..8428)
                     /locus_tag="KRL67_002277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HBH1523151.1"
                     /translation="MRERIILETLKNSSGYLNIDYFADKLGVSTRTIRNEIKKIETIK
                     ECNGFKLEYKSKLGYILNIKDQNKFEHYLKNLPSDLVENPEQRLESIIVELLVNEGYK
                     TIEHLSKKFLVSSSQIKNDLKKIDEKIKDTELKLERKAHYGIKIEGSIKEIQSILVDS
                     YFRGNRNITEYRNKFIDNLKLTNIRGTIKNVLNEHDLEANLTELEEILAQIIILYIRV
                     SMRVARNASDLKLYTEDLIIDELLDKIFGDKKYNLNYDEKSYLKQLIKLRTKDKKATI
                     KNIDKNKLQDIMYEFFREIDKKYNSNFLEDKEFLNLFYLHIACLIERIKKNHKIINPF
                     SVKISQQYPTVFNLSIQFSKIIENEYHIKISQDEIGFIATHIAVPFEKREEANFNKKY
                     KIAIICSSGGGSAFLINLRLKEIFPNAEIRNFSLLEEKAVIEFAPDLIFSITNLLFEI
                     SAPVILINEILDELDYLDIKESVRFADHIGNISSPKQYILGLFDKNHFRCIKEITKYK
                     DILDSMSQCIVDEGACSQTYPKDVWERESYLSTIYTNGVAIPHPIEMTGNKNIISVAL
                     IQTDISYENRVPKIIFMISLIKGNLELHKQISKYLSKIMADKDMVDMLNKSQSYEEFM
                     YKLKIYLGG"
     gene            complement(8756..9607)
                     /locus_tag="KRL67_002278"
     CDS             complement(8756..9607)
                     /locus_tag="KRL67_002278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatY"
                     /protein_id="HBH1523152.1"
                     /translation="MALVTTTSMLKKAQDGGYAVGAFNVENMEMVIAVIEAAEELNSP
                     VILQTTPSTVKYAGLDYYLANAKVAAERVKIPVAMHLDHGSSFDLAMKALRSGYTSIM
                     IDGSHESFENNISLSKSVVKACSPSLIPVEAELGKVGGKEDDLDGGNSNDYTNPKEAK
                     EFVDRTGISSLAVAIGTAHGLYKGEPKIDLDRLSEIKNVVSIPLVLHGGSGIHDNIIK
                     EAIKRGIAKVNYATELRIAYSKGVKKVLNEDSEVIDPKKYGLAGLQSVKDFVKEKIKV
                     CGSVNRV"
     gene            complement(9656..10825)
                     /locus_tag="KRL67_002279"
     CDS             complement(9656..10825)
                     /locus_tag="KRL67_002279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HBH1523153.1"
                     /translation="MKMFKLEEQKLKDLGAIITTKEIKQQPELWLETYEIYKSNKEKL
                     SRFIDTISNNHGQFRVIFTGAGTSAYIGNSILPYLKNKNNIRKYIFEAIPTTDIVSNP
                     YDYLKKDVPTLLISFARSGNSPESLAALNLGNKIVDNFYHLAITCNPEGELAKMTKND
                     ENNYLLLMPSKSNDEGFAMTGSFSCMMLSAMLMFDSLEDDVEKSYINAIIEMGRNVID
                     RKDEIHELINKDFDRVVYLGSGGLGGLTQEAQLKLLELTAGKIATVYDSPMGFRHGPK
                     SFIDEKTLVFEFVSNCSYTRKYDLDVLEEIKRDKIAKFTCAVSVENESNFSGTKFEFI
                     GKYNKLPDVYLAMPYILFAQTIALFVSVKVGNKPDTPSATGTVNRVVKGVTIYEY"
     gene            11296..12213
                     /locus_tag="KRL67_002280"
     CDS             11296..12213
                     /locus_tag="KRL67_002280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HBH1523154.1"
                     /translation="MKNIKVGMIGLGSIAQKAYLPILTKSERFKFVGAFTPNKVKREK
                     ICSDYRIIPFDSIESLAKKCDCIFLHSSTETHYEIIKILLNLGVHVYVDKPLASTVNQ
                     GEELIELSTKKNLNLMVGFNRRFCPMYKEIKNNATEIVSINICKHGLNSLRNVRFDST
                     LIDDYIHVIDTALWLANEDVEISGEDLFLTDNKNLIFVSHKLKGKHFSINTSMHRDSG
                     TKLEQVEILSKGKIQRVKNLNVLEIEESGNLTLKQSGAWVNILKQKGFEDISNHFIDC
                     IENNIKPSISGEECVKAQRLLEKIVSSVK"
     gene            complement(12335..13264)
                     /gene="pfkB"
                     /locus_tag="KRL67_002281"
     CDS             complement(12335..13264)
                     /gene="pfkB"
                     /locus_tag="KRL67_002281"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HBH1523155.1"
                     /translation="MITVITFNPSIDRMYRVNNINIGEVQRVVSKNATAGGKGLNVAK
                     VCKILKENPLAMGFLGGFNGEFIKNELKKLDIQNKFTKIEQETRNCLNIIEDNKVSTE
                     FLEKGPIVEKSDLERFENDIEEVLKNTKILVASGSYCENMPIDYYEKIGNICRANNIK
                     FILDTSGEALKIALKSKPYLIKPNIDEIRHLLGINIESREEIILSGKKLIEMGAENVC
                     ISLGKDGMVYLNKDSVYDVKVPVVKCINTVGSGDSTVAGFSVGILRGYKIENLLKFAN
                     ACGISNALNMETGFVDLGEIDKYQDLVEVKKLS"
     gene            13534..14259
                     /locus_tag="KRL67_002282"
     CDS             13534..14259
                     /locus_tag="KRL67_002282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBH1523156.1"
                     /translation="MIKRNDKRPIYDQLVEILRTKIENEMEPNDRMLSERKICDEYGV
                     SRTTVRLAMAELEHMGYIYKRHGKGTFVAALSQNSQNLMESYSFTDYMRTQGKKPGTK
                     VLSFEVVESTKYFSEKLGITPGEKMIKIMRLRLADDLPMMLERTYLPMKEFIGLTKEK
                     IEQKPLYEIFREDYGEIIKVADEEFSAGIISDKEAKLLEVPGDSPCLKLLRTTYNDDN
                     IVIEFTLSVARSDKFVYRVRHVR"
     gene            14342..15496
                     /gene="nagA"
                     /locus_tag="KRL67_002283"
     CDS             14342..15496
                     /gene="nagA"
                     /locus_tag="KRL67_002283"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HBH1523157.1"
                     /translation="MKSFIYADKFFMKHGIKEKGYLSIINGKFDEFQQERPNITDNVI
                     DFSGKYIAPGLVDTHIHGLLGADVMDNTFEAINTISKGLLKYGVTSFLPTTLTDSVDT
                     LSDSVENISNSFNKVEGAKIQGIYLEGPFFTEKYKGAQNEKYFKDPDINILKLWQRKS
                     KNLIRKIAIAPERKGSIELTKYATKNNISVSLGHSSATFEQVKQVVNSGAKVFVHTYN
                     GMSPLNHREPGMVGAAMVLKDTYAELICDGYHVSPIAVKIMMDAKSSNNIALITDCMR
                     AGAMEDGKYTLGKFNVNVKNSVARLSSGSLAGSVLTMDKAVRNIVDWNISTLEDAIKM
                     ATYVPALSCNIDNVCGSIYQGMDADFIVTDNDFNIHETYINGICKYKVNK"
BASE COUNT         5491 a         2647 c         1927 g         6128 t
ORIGIN      
        1 tatactcata aaatataata aaggagattt taaatgaata tattacaaaa tgaaaattta
       61 attatcgaat caagcagttt tggtgctgaa ctaactaaaa tattttctaa aaaatacgac
      121 aaaaacattc tttgggatgg taataaaaaa tattggggta gacaatcgcc tatattgttc
      181 cctatagtag gtaaactttt tgataatgaa accataatag aagaaaattt atatagcatg
      241 actcagcatg gatttgctag agatatggat tttgagattg tagataaagg agatgcattt
      301 gtatcttaca aattaacaga caatgaatct actttaaaaa agtatccata ttcttttgaa
      361 ctagtcataa actatacatt aaacgataac agtattgggg tagaatggat tgtgaaaaat
      421 actgattcta aagatatatt cttttctata ggtggacatc ctgcattcaa ccttcctttt
      481 tatgagcaaa atgatttttc taaacactac cttgaattta aaagtagaaa tgatgtagaa
      541 aaaatcaatt taaatggttc ttttacagat gatataatat ctattggaaa attaaaaaac
      601 ttacagttaa atccagaagt attcaagaat gatgctttga tatacaccaa catagatgaa
      661 gtatctatat gtaataatga tggcagtaaa tatgtaacta tatctatgga agatttccct
      721 ctagtcggta tttggactcc ttattataat gaaactaatt ctactgctcc ttttttatgt
      781 atagaacctt ggtatggtct tgctgatagt ataaattcta acaaaattta taaagataag
      841 aagtttatta ataaactgag taaaggaaaa gtttttactg cttcttacaa tataaatata
      901 cactagataa aggttaacta acccgttccc aaaataataa aatattttta tataagttaa
      961 tataaggata tgaagtaata ttatttaata ttcctcaata atattcctta atcaaaaaat
     1021 attactggaa tacaaaattc cagtaatatt tgataactaa ataatgtgtt tttatataaa
     1081 ccatagatac cttttttata atttaacaat acatacctat atacgttttt ttgattctat
     1141 ttagagcttc ctttaatgtt tctctagggc atcccacatt aatacgaata aatccatctc
     1201 catctttcac aaaataactt ccaactacca tagttaagtt tgcatgtcta aaaaattctt
     1261 tagattcttc ttcatttcta aacacttctc ttacatctat ccaggctaaa aaagaagagt
     1321 ctgacttcat cacattggct tttggtatat gagtttcaaa gaaatcataa actatttcaa
     1381 aattctcctc tacataacta gtcatagaat ctagccattc ttcgctttca ttgtacgctg
     1441 caacgattgc tggtattgca aatacatttg gagaagttat tgaattgctt tttagtcttt
     1501 ctaataaagt ctccctaatt tttaaatctc ttattaatac atatgacgtt tttaatccac
     1561 ctaagttgaa ccctttatta ggagatacac aaattatgct atttttataa atttctggat
     1621 gagcattcca taaagataca aatctataat ctttaaatat tatatctcta tgaatttcat
     1681 cacttaccaa aattacatta tactttaaac ataactctga cacttgatat agttcttctt
     1741 ttgtccatat acgacctgat ggattttgtg gactacagaa tataagcaat cttatattct
     1801 cgtatttcat ttgattttcc atatcttcta aatctaaata atacctattg ttatctaact
     1861 ttaatgaact acaacacaac tttctattat tatttactat agcctctgca aaagggtcat
     1921 aagcaggtgt atttatcaat acaccctctc cttctttagt atatgcttgg actatataat
     1981 gaagagttga aacagtacca aaagttaatt ttatccagtc tttttctata tacacatcaa
     2041 atcttctttt attccaattt ataatagcat catagaagtc atctcctata aaagtataac
     2101 tataatctcc tactgatgct ctttttaaga tagattcttt tatagcagga gcacactcaa
     2161 aatctatatc agctaaatcc ataggaattg catcttcatt taaaccgaac ttttctttaa
     2221 ttacatggtt gctccatttc ctacaatttc cttccttgcg atttaatact ttatcaaaat
     2281 tatatttcat aattttactc tcctaatatt ataaactctt tagatgtctt attaagtgaa
     2341 acacctttac catttattat aacaatgtca tcttctactc taactccccc tacatttgga
     2401 atataaatac ctggctcaca agacattacc attccctctg ttaatatatc ttcactattt
     2461 ggatttagag tgggaacttc gccatctcca agacctatac catgacccaa tccatgatta
     2521 aaatattcac caaatccata tttatctata atttctctag ccactttgtc aatatcacga
     2581 gcattaacgc cttcctttat tgactctatt gcagataatt gtgcttctaa tactacatca
     2641 tatatctctt taataatctt aggaggttta cctatagata ttgtacgagt catatccgat
     2701 tgatatcctt gataaacaac tccaaaatct atcgttatag cttcatttac tttcaatttt
     2761 ttatttgtag gtcttccatg tggcattgca cctctttctc ctgataccac gattgtttca
     2821 aaagccattc cacttgcacc ttttctcaat gcatgatact gaattagagc tgatacttct
     2881 agctcagtca tatctttttt aatttcacaa acaacctcac taaatacttc atctgtaatc
     2941 tcacaagcct ttttaataag ttcaatttct tctttagatt ttatagccct tagcttccat
     3001 aattcgttgg ttattaaatt gatttcaaaa cctgcatttt ttagtgaaag atactcttgt
     3061 atactcatag cttggttttc tattgctaac ttttttatat gcaattctct taataactca
     3121 attatatctg gtatataaga accttgagat acaacccttt ttatgaatcc agtcgttttt
     3181 ttatctgctt catcaatata tcttccatct ataatttgat agtttttatc tttagtaaca
     3241 agtacgtata caccactacc tgataatgct cctatgtatc ttttagtatt ttttgcttta
     3301 atcaaaagag cgtcaacacc tcttttacag attaaggata ctaatctctc cattgtatta
     3361 ttcataataa aatattagaa atccaaccct aaatcatcca ataagtcttc ttcttcttta
     3421 gagaatatat tcttagtttc ttctctttgt tttttattat tttgttgacg ttccattatt
     3481 ttaaagaatg gccaatataa taaaactgct aatactaatt gtaatgcatt tgctaatata
     3541 gaatgccaat ctccattagt taaaaatcct tctaagaaca ctcccacata tggagggtca
     3601 aatactggtt ttgctaacca tccccaatgc ataaaaaaca ttggtaatgt acctattata
     3661 gctccaccaa atacatgtgg tataaacatt attggattta atattatggg cgctccaaat
     3721 aatattggtt catttatccc aaataatgca gggaataatg acacacgtcc aatcttctta
     3781 taagatgcat tttttgataa catacacgca attacacaac ctatactaat tccacaacct
     3841 gtaaatccaa agaacgcact tgaagctcct gctgtaaaga aatgaggtat tgtttcccca
     3901 gcttgcatag ctgctatgtt ttcagctata aatgtaatca ttataggtct agttataggg
     3961 ctaaatactg atgaatgtat tccaaaaaag aatatagtgc atatagcaaa gttaagtgca
     4021 agaactgacc atgggttatc catacttcct accaatggag ataaaaattg agtaagtatc
     4081 tgtggaggta attctccaaa tacctttaat gaaacaaaac gagcaactat aaaagttcca
     4141 caaactatta tagtggtagg tattaattca aaagacctag acacaaagtc aggtacattt
     4201 tctggcattt ttattgtgat attcttctta ttacaaaaat tatataactc cacagaacca
     4261 agccctgcta tcatacctgt aaatagccct ttagctccta aatttcctat ttctaaagct
     4321 ccatctggtg taaatgaagc tactaggaat aaaaaagaaa aagttgatag tgcaacacaa
     4381 cctggtatat ataatttata gtagttaccc agattaaaag caatacacat acaagcatat
     4441 aatcctatca agccaatgga aagacttact ggtaagtcta atattgctga attattcagt
     4501 atgaatttgc ttattggatt attttctcca agcatatttg gaagtgctgg aagtattgca
     4561 aatatacttc ctaatattgt gaatggcgtc atagcaacca taccagcctt tatagaacca
     4621 aggtgacgtt gattgtcaac cttgtgagct aaaggcataa atactctatt cataaatgct
     4681 tcaaattttg aaaacatatc taatccccct tttccctcat tattattatt atccttgagc
     4741 taattgatgt ttcttataag ttattaatgc caacttcatg actgtagccc catccatttt
     4801 cccatacaca tctttatcta ttactgcaaa aggtttattt tttggctcta atattccttt
     4861 taagaagtct attttgtgag ctatctgagg acctataagt actatatccc aatcatcaac
     4921 ttcactttct attgaatctg ctggtatagc tgtaaactca aaatcttttt cattaagttt
     4981 agcactctct tttacaacct ttttcatatt ttgcattaac atattagtag aaaatccacc
     5041 tgcacaacat aataatattt tcataataat tcctcctaaa gtttgtattt ttattatctc
     5101 ttaaaagtat ctaaactctt aaaagaattt ttacataaac aatctattta tacactacaa
     5161 ctacttatca agacaatata tcaaagcctc tatcaagtct ttcgctagtt gagaagtcat
     5221 atagtggtct tgagcatgta ccattaaaag actcattgct gggctttcaa catcttcatt
     5281 taaatcttga gttattaatt ctgtctgtat tctgtgagcc tcaacatcta ttttacgagc
     5341 ctcctctatt aacaattttg ctcctttaaa atcttttgat tttacttttt ttaaagcctc
     5401 aaaagctttc gatttactct ctcctgcagt tgatataagc tccattattt gtacttctgt
     5461 tgacattttc ttattctcct ttagataaaa tttcttatat gtttatattt ttcttcatca
     5521 aaatttaaaa ttaactgcgt atacaatttt ataatattct ttaaatcttt attacttact
     5581 attgaaaatg cacaatgacc atatctaact ggtacacaag ttactacaca aggtttccct
     5641 tcatttacaa gatgtgcttc tcctccatca gtcccacctg attcaaacat atcatgttga
     5701 agtggtatat tattttcatc agcaattttc attacataat ctatcatttt tctactaggg
     5761 actaaagtcc tatctctatg cattagcatt acacctctat caatttgttt ctgatttgta
     5821 taatctctca caaactcatt cttagcacat gctacatcta ttacaaatac tatatcggga
     5881 tttattaatt gtgttgatgt ctttgccccc cttattccaa cttcttcact acttgtagct
     5941 gcaaagtgaa tattacaatt tatattttct ttcttaagct cttttaaaac ttctcccatc
     6001 acaaaacatc ctatcctatc atcaaaagct tttcccacta atcggtctgg caaagtaaat
     6061 tcctcaaagc ttgttgtata tgttaccata tccccaacgt taatgcctaa actatataca
     6121 tcttcttctg tatatgctcc aatatctgca tatatatttt cagccttacc attattatat
     6181 gtagcattta tgatagcagg gatttttttc ccttctcttg ttgtaattct aattttttgt
     6241 gtaaactgtg caagaggttt tactcctcct attgttatta agtgaatcat accaagttta
     6301 tctatacttc tcaccataaa tccaacttca tccatatgag cacaaatcat tatatttggt
     6361 gcactttcat cttttatttt agaaaagatt atactcccta gcccatcaca tataacttta
     6421 tcactataag cttttagctc ctctaataaa acatgtctaa cctcttcttc attagatgct
     6481 atgccatctg cttctgataa cctcttaaaa aactctatat tcatgtctaa cctcccagat
     6541 agatttttaa cttatacata aactcttcat aagattgtga cttatttagc atatctacca
     6601 tatccttatc tgccataatc ttacttaaat atttagatat ttgtttatga agctctagat
     6661 ttccctttat taatgaaatc ataaaaataa tctttggcac cctattttca taactaatat
     6721 cagtttgtat taaagctact gatataatat ttttattacc tgtcatttca attggatgag
     6781 gtatcgccac cccatttgta taaatagtac ttaaataact ttctctttcc cacacatctt
     6841 tgggataagt ttgagaacaa gctccctcat caactataca ctgtgacata ctatcaagga
     6901 tatccttata cttggttatc tcttttatac acctaaagtg gttcttatca aacagaccta
     6961 atatatattg ttttgggctt gatatatttc ctatatggtc tgcaaatcta acactttctt
     7021 tgatatctaa ataatccagt tcgtctaaaa tttcatttat aagtataact ggagcgctta
     7081 tttcaaataa aagatttgtt attgaaaaaa ttaagtctgg tgcaaattct attacagctt
     7141 tttcttctag caatgaaaaa tttcttatct cagcgtttgg aaagatttct ttaagtctta
     7201 aatttatcaa aaatgcactg cctccgccag aagaacatat tattgctatt ttatattttt
     7261 tattaaaatt agcctcttct ctcttttcaa agggaacagc aatgtgagta gctataaatc
     7321 ctatttcatc ttgacttatt tttatatgat actcattttc tataattttt gaaaattgaa
     7381 tagataaatt aaaaacagtt ggatactgtt gacttatttt aactgaaaaa ggatttatta
     7441 tcttgtgatt cttctttatg cgttctatta aacatgcaat atgtaaataa aataagttaa
     7501 gaaactcttt atcttctaaa aaattagagt tatatttttt atctatctct ctaaaaaatt
     7561 cgtacataat atcttgaagt ttattcttat caatattctt tatagtggct ttcttatctt
     7621 tagtccttaa ttttataagt tgctttaaat aagacttttc atcataatta aggttatact
     7681 ttttatctcc aaaaatttta tctaataatt catctataat taaatcttct gtatataatt
     7741 ttaaatcact agcatttctt gctactctca tactaactct tatatacaat ataataattt
     7801 gtgctaatat ttcctcaagc tctgttaaat ttgcttctaa atcatgctca ttaagtacat
     7861 tttttattgt acctctgata ttagttaatt ttaaattatc tataaactta tttctgtact
     7921 ctgttatatt tctattccct ctaaaataac tatcaaccaa tatactttga atttctttta
     7981 tagacccttc tattttaatt ccataatgag cctttcgttc caattttaat tctgtgtcct
     8041 ttatcttttc atctatcttt tttaaatcat tttttatttg agatgagctt actaaaaatt
     8101 ttttacttaa atgttctata gttttataac cttcatttac taaaagttct actattatag
     8161 actctaaccg ttgttctgga ttttcaacta aatctgaggg taagtttttt aggtaatgct
     8221 cgaatttatt ttggtcttta atatttagga tatatcctaa tttagactta tattctaatt
     8281 tgaaaccatt acattctttt atagtttcta tcttttttat ttcatttctt atagttcgag
     8341 tagatacccc caacttatct gcaaaataat ctatgtttag ataaccacta ctatttttta
     8401 atgtttcaag aataatgcgt tctctcatat ccccacctcc ctcagtgtta ttattgtatt
     8461 ttcccaaata gtataaaaaa tattttctta taaaatcaac cttttattgt tatttataga
     8521 attttatatc ctagtatatt ttagcgctaa aatttaatat acatatttca taatagtaaa
     8581 atgtatagtt caatttttat tatattttaa ttatatatgt aatactttac caataaaagc
     8641 tgatattttt catcaattaa atgaaaaaaa tacaccattt acctataatt aatgtaaaat
     8701 tttcatataa caaatatcct aaacttatct aaaataaatt taggatattt tatttttata
     8761 ctctatttac agacccacac actttaatct tttcttttac aaaatccttt actgattgaa
     8821 gccctgctag tccatacttt ttagggtcta taacctcaga atcttcattt aaaacttttt
     8881 taaccccttt actatatgca attcttaatt cagttgcata gttaactttt gcaatacctc
     8941 tttttatagc ttcttttata atattatcat gtattcctga acctccatgc aatactaaag
     9001 gtattgaaac tacattttta atttctgata acctatctaa atctatttta ggttcaccct
     9061 tatacaatcc atgtgcagta cctatagcaa cagccaatga agatattcct gttctatcga
     9121 caaactcttt tgcctctttt ggatttgtat aatcattact attcccgcca tctaaatcat
     9181 cttcttttcc accaactttc ccaagctctg cttccactgg tataagagaa ggactacatg
     9241 cttttactac tgatttagat agagatatat tattttcaaa actttcatga gaaccatcta
     9301 tcattattga tgtatatcca cttcttaaag ctttcattgc aaggtcaaaa cttgaaccat
     9361 ggtccagatg cattgctaca ggtatcttta ctctttctgc cgcaaccttt gcatttgcta
     9421 gataataatc gagacctgca tattttacag tagatggagt agtttgtaat attactggag
     9481 aatttaactc ctcagcagct tctataacag caattaccat ttccatattt tccacgttaa
     9541 atgctcctac tgcatacccc ccatcttgtg cctttttaag cattgatgtt gttgttacta
     9601 gtgccacatt gataccctcc caaattattt agttataata ttttaatctt ctcttttaat
     9661 attcatatat tgttacacct tttactactc gatttactgt tcctgtagct gatggcgtat
     9721 ctggcttatt tccaaccttt accgatacaa acaatgctat agtttgtgca aacaatatgt
     9781 atggcattgc aagatataca tctggtaatt tattatactt tcctataaac tcaaactttg
     9841 ttcctgaaaa gttactttca ttttctactg acaccgcaca agtgaattta gcaattttat
     9901 ccctttttat ttcctctaaa acatctaaat catattttct tgtatatgag caattagata
     9961 caaattcaaa tacaagtgtc ttttcatcaa taaatgattt tggtccatgt ctaaacccca
    10021 taggagaatc atatactgta gctatttttc ctgctgtaag ttctaaaagt tttagttgtg
    10081 cttcttgtgt caaaccacct aatcctcctg aacctaaata tacaactcta tcaaaatctt
    10141 tatttattaa ctcatgtatt tcatcttttc tatctataac atttcttccc atttcaatta
    10201 ttgcatttat atatgacttt tctacatcat cttctaaact atcgaacatt aacatagcag
    10261 ataacatcat acacgaaaaa cttcctgtca tagcaaagcc ttcatcattt gattttgatg
    10321 gcattaataa caagtaatta ttttcgtcgt ttttagtcat cttagccagt tctccttctg
    10381 ggttgcaagt tatagctaga tgataaaaat tatctacaat cttattccct aaatttagag
    10441 cagccaaact ttcagggcta tttccacttc ttgcaaatga aattaataat gttggtacat
    10501 ctttttttaa gtaatcatat ggattagaaa ctatatccgt agtaggtatt gcttcaaata
    10561 tatattttct tatgttattt ttatttttaa ggtaaggaag tattgagttt cctatgtaag
    10621 ctgaagttcc agcacctgta aatataactc taaactgccc atgattatta cttattgtat
    10681 ctatgaatct acttaatttt tctttgttag atttatatat ttcataagtc tctaaccata
    10741 attcaggttg ttgttttatt tctttagttg ttattattgc acctaaatct tttagttttt
    10801 gttcttccaa tttaaacatt ttcattctcc tttcatactt gtaacgttta gatttggtaa
    10861 tgaggttata accactttaa attataaaaa aatatactct tttgtaattt tttacttaga
    10921 ttttttagca ctacttaaac tcactataaa atatacgcta tagattttaa ttaattttat
    10981 agtttatctt taaaaattta ataaaatcat actctaagat gctttagtat ttttatttta
    11041 tgataaaaac ttgttcagac caaagtggta attaccacta atccaaatat aaattaattt
    11101 gaattttttg tcaatacata aaatatttat atcaatcaaa tttatagata tcctagaaaa
    11161 ttactttatt aatatttctc attttcctaa tttacttata taagctttac atttattttt
    11221 gatacaaagt ttacaaattt atgtgtctga attataatca attctgtaaa tataaaaacc
    11281 ttaggagaaa gtattatgaa aaatataaaa gtgggaatga taggtcttgg ctccatagct
    11341 caaaaggcat atttacctat attaactaag tctgagagat ttaagtttgt aggagctttt
    11401 actccaaata aagttaaaag agaaaaaatc tgtagtgatt atagaatcat accattcgat
    11461 tctattgagt ccctagctaa aaaatgtgat tgtatttttc tacatagctc tacagaaaca
    11521 cactacgaaa ttataaaaat attattaaat ttaggtgttc atgtatatgt agacaaacct
    11581 cttgcttcaa cagtaaatca aggagaagaa cttatcgagt taagtacgaa aaaaaactta
    11641 aatttaatgg ttggattcaa ccgtagattc tgtcctatgt acaaagaaat taaaaataat
    11701 gctacagaaa tcgtatctat caatatatgt aaacatggat taaattcctt aagaaatgtt
    11761 agattcgaca gtactcttat agatgactat atacatgtta ttgacactgc tttatggtta
    11821 gcaaatgaag atgttgaaat tagtggagaa gatttatttt taacagacaa taagaatctt
    11881 atatttgtaa gtcataaact taaaggtaaa catttctcta taaacacctc tatgcataga
    11941 gattctggaa caaagttaga acaagttgaa attctttcaa aaggtaagat tcaaagagtt
    12001 aagaatctta atgtactgga aatagaagaa agcggaaacc taactttaaa acaaagtggt
    12061 gcatgggtaa atatattaaa acaaaagggt tttgaggata tatcaaatca ttttattgat
    12121 tgtatagaaa ataatataaa accatctatt agtggagaag agtgtgtcaa ggctcaaaga
    12181 ttactagaga aaattgtaag ttcagtaaaa taaatattta ttataaaaat aggttaaaga
    12241 tgaattctct aacctatttt tatataaaac tatatctttt atattaaaat ctattacaat
    12301 aacattaaac ttattatatt aacattaaat gtatttaact taactttttt acttcaacta
    12361 aatcttgata tttatcaatt tctcccaaat ctacaaatcc agtttccata tttaaagcat
    12421 tacttatacc acaagcattc gcaaatttaa gtaaattttc tattttataa ccccttaaaa
    12481 tacctacact aaatccagcc actgtgctat ctccactacc tacagtattt atacacttta
    12541 caactggtac ttttacatca tatacactat ctttatttaa atacaccata ccatcttttc
    12601 ctaaagatat acatacattc tctgcaccca tttcaataag tttttttcct gacaagataa
    12661 tttcttctct tgattctatg tttattccta ataaatgtct tatttcatct atatttggct
    12721 ttattaaata cggttttgat tttaaagcta tttttaatgc ttctcctgat gtatctaata
    12781 taaattttat attatttgct ctacatatat tgcctatctt ttcataatag tctatcggca
    12841 tattctcaca ataactacca gaagccacta gtattttagt attttttagt acttcctcta
    12901 tatcattttc aaatctctct aaatcgctct tttctactat tggtcctttt tctaaaaatt
    12961 ctgtactaac tttattgtcc tcaattatgt ttaagcaatt tctagtttcc tgctctattt
    13021 ttgtaaactt attttgtata tctagttttt taagttcatt ttttataaat tctccattaa
    13081 atccacctag aaatcccata gctaaaggat tttcttttaa aattttacat accttagcta
    13141 cgtttaatcc tttacctcca gcagttgcat tcttacttac taccctttgc acctcaccta
    13201 tatttatatt atttactcta tacattctat ctatagatgg gttaaatgtt attactgtaa
    13261 tcatactaga ttctcctcat tattaatttt tcacttttct aaacaataaa ttaatctgta
    13321 aaataaaaaa tacttcgtct ataatatact caaagtggtt attaccaatt tgaatataaa
    13381 ttattttaat tgttttgtca atgtatcata attttataat aaattaatga ccttacttat
    13441 tgaattttga ttaaacttta ttcattgaca taaataattc tatattctat aatcaaattg
    13501 tggtaataac cacttagaaa ggactatatt tttatgataa aaagaaatga taaaagacca
    13561 atatacgacc aacttgttga gatattaaga actaagattg aaaatgagat ggaaccaaac
    13621 gatagaatgc tttccgaaag aaaaatttgt gatgaatatg gagtaagtag aaccacagtt
    13681 agactagcta tggctgagtt ggaacatatg ggatatatat acaaaagaca tggaaaagga
    13741 acttttgttg ctgcactaag tcaaaattct caaaacttaa tggaaagtta tagctttaca
    13801 gattatatga gaacacaagg aaaaaaacca ggtacaaaag tattatcatt tgaggtagta
    13861 gaaagtacaa agtatttttc tgaaaaatta ggtataactc ctggtgaaaa aatgataaaa
    13921 ataatgcgtt taagacttgc tgatgacctt cctatgatgc ttgaaagaac atatctccct
    13981 atgaaagaat ttataggatt gacaaaagaa aaaatcgaac aaaaaccttt atatgaaata
    14041 tttagagaag attatggtga gattattaaa gttgctgatg aagaattttc agctggaata
    14101 atctctgata aggaagctaa gttattggaa gtaccaggag attcaccttg tttgaaatta
    14161 cttcgtacca catataatga tgacaatata gttattgaat ttactttgag tgttgctaga
    14221 agtgataaat ttgtatatag agtaagacat gtaagatagt aaattaaata gctttcaaga
    14281 aaacagaatg atattataaa cgtcaatgcc acaatgatag tataattata aggaggttat
    14341 tttgaaaagt tttatttatg cagataaatt ttttatgaaa catggaatca aagaaaaagg
    14401 ttaccttagt ataattaatg gcaaattcga tgaatttcaa caagaaagac ctaatattac
    14461 tgataatgtt atagattttt caggtaaata tattgctcct ggcttagttg atactcacat
    14521 tcatggtctt ttaggtgcag atgtcatgga caatacattt gaagcaataa acacaatttc
    14581 aaaaggctta ttaaaatatg gagtaacatc tttcctacct acaactttaa ctgattctgt
    14641 agatacacta agtgattctg tagaaaatat aagcaatagt tttaataaag ttgagggagc
    14701 aaaaatacaa gggatttatt tagaaggtcc gttttttaca gaaaaatata agggcgctca
    14761 aaatgaaaaa tattttaaag accctgacat caatatatta aaattatggc aaagaaaatc
    14821 gaaaaattta ataagaaaaa tagcaattgc tccagaacgt aaaggttcaa tagaattaac
    14881 aaaatatgca actaaaaata atatctctgt ttctcttggc catagttcag ctacatttga
    14941 acaagttaaa caagtcgtta attcaggtgc taaagtgttt gtacatacat ataatgggat
    15001 gagcccttta aatcatagag aacctggaat ggttggagct gctatggtgc taaaagatac
    15061 ttatgctgaa ttaatttgtg atggttatca tgtaagccca atagctgtta aaataatgat
    15121 ggatgcgaag tctagtaata acatagcttt aattacagat tgtatgaggg ctggtgctat
    15181 ggaggatgga aaatatactc ttggtaagtt taatgtaaat gtaaaaaata gtgttgcaag
    15241 attaagtagt ggtagtttag ctggaagtgt attaactatg gacaaggctg tgagaaatat
    15301 agttgattgg aacatatcaa ctttagaaga tgcaataaaa atggctacat atgtccctgc
    15361 tttaagttgt aatattgata atgtctgtgg ctctatatat caaggtatgg atgcagactt
    15421 tatagtaaca gataatgact ttaatataca cgaaacttat ataaatggta tatgtaagta
    15481 caaagttaat aaataggttc aatataaggt tatggaggct gtctaaaaag agtctggaaa
    15541 tatttaaagt tatttctcca aaatctgatt taacactttt atttccatat ctttaatata
    15601 ttattgcgat agaggttatt ttaaacaaat atatattata agtatttttg tgataatatt
    15661 ttcgataata tgtaaattta aagtaacttt tctattattt gtaagttttt atcttgagca
    15721 aacatataaa tttttaataa agcatgagat aaaccctata tttcaattac taatgatgag
    15781 attttgattg ataacagata tattttcaaa tgtaaaaata ctaaaaatca tatcaatttt
    15841 aaagatattg taggtgtaaa taaacaagat aatactatag aaatttttac aaatgaaagc
    15901 acttattgta tatatctaaa aattttaagc attgagggcg aagaatatct aatagataga
    15961 ctaaaatcat tttgttgatt ttatatattt tatgttaaat ataaagcaga gtagttaaaa
    16021 ctaccctgct tttgtaatta aaatttaaaa ataataactc ataatatatt gaatttgagc
    16081 ttaacagtaa ctattgtaat aatagcatca atcttttgtg ttgaatataa tttatttaca
    16141 ctatagttaa gcatgatttc taatgttatt ttctctaatt tacaattatg att
//
LOCUS       DAEPXF010000084        15824 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain HB27
            SAMN13376389-rid12479423.denovo.088, whole genome shotgun sequence.
ACCESSION   DAEPXF010000084 DAEPXF010000000
VERSION     DAEPXF010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13376389
            Sequence Read Archive: SRR10518432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 15824)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15824)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001069624.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001069624.1
            Genome Coverage       :: 315x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/15/2021 01:54:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,165
            CDSs (total)                      :: 4,076
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 89
            rRNAs                             :: 10, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 10 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 19 of 69
            Pseudo Genes (incomplete)         :: 44 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 10 of 69
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15824
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13376389-rid12479423.denovo.088"
                     /strain="HB27"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="China: Hebei"
                     /collection_date="2017"
     gene            212..1429
                     /locus_tag="KRL67_002284"
     CDS             212..1429
                     /locus_tag="KRL67_002284"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase"
                     /protein_id="HBH1523158.1"
                     /translation="MMNVTLTDVKEARETIKNIVKRTDLLESVKLSEKTGANVFYKCE
                     NLQKTGSFKLRGACNKIASLTDEEKANGVIASSAGNHAQGVALGAKMTGIKSTIVMPA
                     TAPLAKVSATKGYGADVVLNGAVYDDAYAKAVEIQKETGATFLHPFNDKYVIAGQGTI
                     SLEIFEQLDNKVDTILCPVGGGGIISGVAVAAKALNPNVKIVGVQTANIPSMKESIKN
                     GKVTTAFNDTTIADGIAVKTPGDLTFEIINELVDEIVVVEETEIAESILFMMESQKIV
                     SEGAGAVCTAAILSGKYVPAKDENVVCIISGGNIDINTLYRIIGVALAKEGRRYSFST
                     IMEDKPGNFAELTRIISENGGNILSANQGKLSAGEALGKQSAEFILETIDYDHIARIK
                     KAIEEKGFKIIEL"
     gene            1524..1904
                     /locus_tag="KRL67_002285"
     CDS             1524..1904
                     /locus_tag="KRL67_002285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HBH1523159.1"
                     /translation="MKHEVIHTNDAPAALGPYSQAIKAGNLLFVSGQVPLVPETMEVV
                     EGDVQAQTAQSLKNLKAILAESGADFSNVVKTTVFIKDMNEFGAINEVYAEYFGENKP
                     ARACVEVARLPKDVKVEIEVIAVL"
     gene            2187..2669
                     /locus_tag="KRL67_002286"
     CDS             2187..2669
                     /locus_tag="KRL67_002286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HBH1523160.1"
                     /translation="MLNFFKKNKSYKLHAVVSGNSINIEKVNDSVFSKKLMGDGVAII
                     PNSNVVVAPCNGKVTVLTESKHAFGMVSDEGVEILVHIGIDTVNLQGEGFKNEVSQGD
                     TVKKGSPIISFEREKINSQGIDCTTIMVVLNHSEFSEINCMVENEVVAGKDTVIEIMK
                     "
     gene            2690..3505
                     /locus_tag="KRL67_002287"
     CDS             2690..3505
                     /locus_tag="KRL67_002287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HBH1523161.1"
                     /translation="MRVVKIFNNNALSTVTNDRKEAILLGLGIGFNKRPGDKVNEDKI
                     EKIYYVQDHMQTKFLELLKNVSPEVMDASQQIISLIPGNEGKFNNKGILSLVEHISFA
                     IDRMKNNVFLPNLMLLDIKMMYSKEFELGVKALEIIYRVCHIHLPEDEAGYIALHFVN
                     LQSNDNLAYDTLKFVKGSIDLIKECYGLELDESSLSTLRFRTHLKFLAQRIFKNEICQ
                     DDKMIEMYDYLINNHPKNKEYLEKLNVYIEKEFRYKLDKPEKIYLLLHLTKIL"
     gene            3558..5108
                     /locus_tag="KRL67_002288"
     CDS             3558..5108
                     /locus_tag="KRL67_002288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HBH1523162.1"
                     /translation="MKEKLLGGLETFAKAMVQPLMYLSSAGILMVVGVLLTNDMLLGV
                     LPFLKWEPIQIFGSLIYNCIMAIINNLAVIFAIGIPAALAKKEKHKAALIGFMSYIIY
                     LTASNTMLTSLGQLAKESKMLGLIGTGQATILGIQVVDTSVFGGIILGCIAGYVFNRT
                     SNKTFKGALQIYSGANFSFICMFFVSILIGVVSNFIWPLAQQEIASLANVIKDTGTFG
                     LFLYGFLERILVPTGLHHLIYTPFQFSDLGGVLKVGADTVSGAYAIVMTEMNLPVERF
                     SDSIYYMATGFTKMFGYIGIGAAFIYTAYKQNKKKVKATVIPLIITAVLASVTEPLDF
                     MFVFCAPLLFFVHAVIAGLFIALLKIFDVTALCGGNLLVSIIMNTTVGVEKTHWPVMM
                     ILGLAQIIVYFVVFSFLIKKFNYKTPGREDESTATGEEQSKQLNLDAGIENIIDGLGG
                     KENINTVENCITRLRVNVKDESKINEDIINLTPNSGIVRKGKDIQIIFGLHVHEVRRA
                     VEDFLEAY"
     gene            5207..6559
                     /locus_tag="KRL67_002289"
     CDS             5207..6559
                     /locus_tag="KRL67_002289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-alpha-glucosidase"
                     /protein_id="HBH1523163.1"
                     /translation="MIITIVGGGSTFTPGIVKSIALREKELELDEIRLFDIDKERQDK
                     VAVVVNWILKEDVKTDVKLTVTNCEKEAYTDATFIFAQMRVGKYEMREQDEKIPLKYG
                     CVGQETCGCGGMAYGLRTIQPMIKVIDDVEKYAKKDYWILNYSNPASIVSEACRKLRP
                     NARIINICDMPIAIIDMVAGSLNINDVHNIRYDYFGLNHFGWFTSIDYKHRDLMEEIK
                     EYIKENKILLPKSYIENLNKSRSTGNRHAVSSWIHVWESVYTMLESFPKYLPNTYLTY
                     YLLSKETVEHSNINRTRANEVMESREKDLFDGVRHYLETGEISEKAFYAGSHGDWISD
                     LAVSIKNDTRSRFLVITENRGAIPNMPYDAMVEISAYIGKDGPEVIARGPIPLFQQGL
                     MMQQLNSEKLIVEAYIENSYEKALQAFTLNKTIPSMNVAKEILDEMIEVNKEYWPELR
                     "
     gene            6690..7448
                     /locus_tag="KRL67_002290"
     CDS             6690..7448
                     /locus_tag="KRL67_002290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBH1523164.1"
                     /translation="MNIINKIFWQEDRIGMITNNLEADTHSHCMLQLFLGIEDSIEIT
                     VNEKLVKCNCIIVDKNISHSFSARKKVYYSAIIEPTSIYAEQLTSKMNDFGYWICDND
                     GLEKLRQQGTFLIYNSSKEQYLRFMEMLNNYLNIPITLKHYDDRITELLNLLRTCNCD
                     NHTISSFADKVSLSASRLSHLFKEQIGIPLKSYILFHQMECAFRELLSGKNVTEASMT
                     AGFDTPSHFAGTVKRMMGMPVSLSLKDSEFLKVY"
     gene            7495..8214
                     /locus_tag="KRL67_002291"
     CDS             7495..8214
                     /locus_tag="KRL67_002291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
                     /protein_id="HBH1523165.1"
                     /translation="MNKEYLEETKMLNYNEPQLKLLVSSKKWLELDDFHKIKSIYEFV
                     QNDILFGYNTSDMLSATQVLNDGMGQCNTKATLLMALLRAVNIPCRLHAFDVTKDFQR
                     GATSKLISLLAPKYILHTWVEVFYQDRWIALEGVITDKKYLEAIQKKFFNHDGTFKKY
                     AIATNDLKNTSIDWNGKDTFIQKEAIVYDYGIFPSPDVFFSTHSQHMSKLKNFIYVHL
                     IRKIMTKNVCKARNNYIDKNE"
     gene            complement(8338..9738)
                     /locus_tag="KRL67_002292"
     CDS             complement(8338..9738)
                     /locus_tag="KRL67_002292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HBH1523166.1"
                     /translation="METQSNNKKGNLIIAIVMTGAFISSLSQTLLSTALPNIMSDFKI
                     TADVGQWLTTIYLLIAGIIVPTTAYLINRFSTRKLFITSMSIFSIGCIIALFSNNFST
                     MLVARVLQAMGSGSLMPLLQVIILYLCPEEKRGAAMSLVGITVGFAPAIGPTLSGWLV
                     DSFGWHSLFLFLSPIAILDVILSFILLKNVGETQNLKLDIPSIVLSSLGFGGLLIGFT
                     NQGNYGWTNIATYLPILIGIMSLILFTLRQLKSKEPFLELRVFKNKPFLISTILIMIV
                     YASMMSATLMIPLYVQSVRGFSALSSGSLMLPGAILMVVLNPIAGRHLDKYGPHALSI
                     LGTGFLFLGTLSFAFLGRDTSLIHVSLMYCIRMIGISMVLMPLTTWGIKTLDRELISH
                     ATAINNTLRQISGAIGSAILITIMTSATKKAHMSSNMLSNIHGIDVAFSIAATLAFTG
                     LIVSICFIKRYQIIRS"
     gene            complement(9742..10650)
                     /locus_tag="KRL67_002293"
     CDS             complement(9742..10650)
                     /locus_tag="KRL67_002293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBH1523167.1"
                     /translation="MKGDNKVTEKQMLIITIAQKIFDQKGFQNTSISDVVKECKMSKA
                     TFYKHFETKESFICEIINYYDEKFLEIIHSINENNDIPSSEKLNRKIIAVWKNIFSRT
                     TINTYIRENFSEEQRKATSKLQTKSRANLLNEYKLSLFDNYGDKIEKIIFDLVFLLDA
                     LIHQFIYIIHVQKREINVYFIAKFTIQILDLVVENMDNLNPLIEKSMFLHKQEDEVYF
                     SLHNKSLFFKTIQDIEELIKTDTSLLENPKLLEAVKKLYVEGKNQQYDSLIMDAMIAY
                     LEKEDTLRPKVLLLNSIKNQLKKETL"
     gene            10966..11613
                     /locus_tag="KRL67_002294"
     CDS             10966..11613
                     /locus_tag="KRL67_002294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HBH1523168.1"
                     /translation="MKKKICVAIVFALFIVFAKMFIDSSSEERNVMKNELSKYRSGVN
                     VLSVNNIIVANKKYSLPENYSPQESIEARDAFFKMNEDAQKSGLNLKAFSTYRSYEYQ
                     DRLFKSYIKEHGEEEANRFSAKPGESEHQTGLAFDIGGDDKSCWANQKFNNTKEAKWL
                     YENAYKYGFILRYPEGKEDITGYMYESWHYRYVGTEHSKNFAMNNLTLEEYLHIN"
     gene            11938..14577
                     /locus_tag="KRL67_002295"
     CDS             11938..14577
                     /locus_tag="KRL67_002295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HBH1523169.1"
                     /translation="MSYKSTYQEVIKNLDSSLSGLSKERAEELLEKNGANELKEADKV
                     PTYKLFLESFKDPLVIILLIAALVQIFLGETVESIIIFAVIIINSVLGVVQTKKAESS
                     LESLKNLSAPNAKVIRDNKKMTIPAKNLVVGDIVFLEAGDYIPADGRLIEAQSLKVVE
                     GMLTGESEPVLKHTEKISEDVSLGDQKNMVFSGAVVVYGRGSFVVTGTGMDTEMGKIA
                     GLLESAENKLTPLQQKIEDFSKKLGVGIVILAILIFIIQVARNYFMAGNGEMTQIILD
                     SFMFSVAVAVAAIPEALSSIVTIVLAVGTNSMAKKQAIIRKLPAVETLGSTSVICTDK
                     TGTLTQNKMTVVDYYMYGTNNDELDKEKVNYSYHAKLLTMVSTLCNDSHITSDGKEVG
                     DPTEVALINYSSKNDLDYDKLRSKYIRINEIPFDSDRKLMSTVNSIYGDYIMFTKGAP
                     DIVFSRCKYALKNGSKVDITDEIIEEYKHKNEEFSNRALRVLAFAIKDVPEEDFIPSI
                     DDEHDMTLVGIMAMIDPPREEVMDAVKEAKSAGIKTVMITGDHKTTAAAIAKEIGIME
                     EGDLALTGKELDALSEEQLYEKLENISVYARVSPENKIRIVKAWQHKNNITAMTGDGV
                     NDAPALKQADIGIGMGSGTDVAKDASAMVLVDDNFASIVNAVEVGRTVYSNIIKSITY
                     LFAGNLGAIVSILFAVFAGWSNPFTALQLLFINLVNDSLPAIALGFEKSEKNIMNKPP
                     RHPDESILAGGTLKAVAIRGCIIGVVTIIAQYIGLQVSPELGVAMAFSTLILARIMQT
                     LPARSNNQTVIKLGFFSNKYVIGAVLVCLGLYSLTLIPVLRGIFSIPTTFGLYQFSIC
                     LGLALISSILMELNKLINRSEVK"
     gene            complement(14635..15738)
                     /locus_tag="KRL67_002296"
     CDS             complement(14635..15738)
                     /locus_tag="KRL67_002296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate aminotransferase family
                     protein"
                     /protein_id="HBH1523170.1"
                     /translation="MKLKINKAIEMQLKKSYAIESGHSEDVFEIDCGEGINTVSYSNK
                     AVEAFNALKFDMIRGYPHSIALKDNIVDYWKDFITLDTDRICLADGSIHVIYLLNRLF
                     IEKGDKVLGYSPQFSEYETDIKMHGATYDYVLLKKEDNFKFNEKEFIEKINPEYKVIY
                     IDNPNNPTGQIIPLSSIENIVKEAAKYDIAVMVDEAYGEYMPKENSAVKLLNNYDNVI
                     ALKTFSKGFGLAGLRAGYAVLPEQLVSPIKKISTPYEVSEISRSIAANLLDDVQFIEE
                     LKEKTKDIKNQLLIPWKNLNIAETSDTVSIMTVEHKNKDIDLQQEFAKLKIRVISGSD
                     FTGLDKNFIRFRMPEEKELPKVIKAFQIIDNIE"
BASE COUNT         5968 a         1886 c         2740 g         5230 t
ORIGIN      
        1 agaatgtaaa aaatattttt aaattatcat ttaatgtaat ttaaatgaat tgaagatata
       61 tgtttattgt atgcaattgt tgtattttta ggttttttgt gattaattaa aaaattataa
      121 aaatcgttta catgttgatt tttaaaaaaa aaagatatag aattatatag aaagtaataa
      181 acaaaaattg gatttacggg agtggaatga aatgatgaat gttaccttaa cagatgtgaa
      241 agaggctaga gagaccatta agaacatagt aaaaagaact gacttacttg aaagtgttaa
      301 acttagcgaa aaaacaggag caaatgtgtt ctataaatgt gagaatcttc aaaaaacagg
      361 ttcttttaaa ttaagaggag cttgtaacaa aatagcgagt ttgacagatg aagaaaaagc
      421 aaatggagtt atagcttcaa gtgctgggaa tcatgctcaa ggagttgcat tgggggcaaa
      481 aatgactggt ataaaatcta caatagtaat gccagcaact gcacctttag caaaagttag
      541 tgcaactaaa ggttatggag cagatgtagt tttaaatggt gcggtttatg atgatgcata
      601 tgcaaaagct gttgaaatac aaaaagaaac aggagcaact ttcttacatc cattcaatga
      661 taagtatgtt atagcaggac aaggaactat atctcttgaa atttttgaac aattagacaa
      721 taaagttgat actatactat gcccagttgg tggaggagga atcatctctg gtgttgctgt
      781 tgcagcaaaa gcattaaatc ctaatgtgaa aatagttggt gttcaaactg caaatatacc
      841 ttcaatgaaa gagtcaataa aaaatggaaa agtaacaaca gcatttaatg atactacaat
      901 agctgatggt atagctgtta aaactccagg agatttaact tttgaaataa taaatgaatt
      961 agttgatgaa atagtagtgg ttgaagagac agaaattgca gaatcaatat tatttatgat
     1021 ggaaagtcaa aaaatagttt cagaaggtgc tggtgctgta tgtacagctg ctatattaag
     1081 tggtaagtat gtaccagcaa aagatgaaaa tgtagtttgt ataatatctg gtggtaatat
     1141 agacataaat acattatata gaataatagg tgttgcttta gctaaagaag gaagaagata
     1201 ttcatttagt acaattatgg aagataaacc aggaaacttt gctgaattaa ctaggataat
     1261 aagtgagaat ggtggaaaca ttttaagtgc taatcaaggt aaattatcag caggggaagc
     1321 tctaggaaaa caaagtgcgg aatttatatt agagacaata gattatgacc atatagctag
     1381 aataaagaaa gctatagaag aaaaaggttt taaaattata gaattataat aaaaagtgta
     1441 tataatatac aagaaaaata gtataatagt gtatgaagat acattttatg tgtattagat
     1501 taacgaatta ggaggatttt aaaatgaaac atgaagtaat acatactaat gatgcaccag
     1561 cagctttagg accatattct caagctataa aagctggaaa cttattattt gtatctggac
     1621 aagttcctct tgtaccagag actatggaag tagttgaagg agatgtacaa gctcaaactg
     1681 ctcaatcttt aaaaaatctt aaagctatat tagctgaaag tggagctgat ttttcaaatg
     1741 tagttaaaac tacagtattt ataaaagata tgaatgaatt tggggctata aacgaagttt
     1801 atgctgaata ttttggagaa aataaaccag caagagcttg tgtagaagtt gctagattac
     1861 caaaagacgt taaagttgaa atagaagtaa tagctgtatt ataaaaataa atacttttaa
     1921 tttgaaaagg ctgaagaact atattctagt gattcagcct ttttataaaa aaataaatca
     1981 caataaagag catgataaat attgtcgaat taaaaaataa aattaaaata aatattgcaa
     2041 tatttttgag aaggttttat aatgagtata taatttgaaa cgtgtaacta ttaaattagg
     2101 catgagttct tggatatttt tatcctgagg attcatgcct tttttgtatt gaaagtcaaa
     2161 aatttattta ataaggagga agtactgtgt taaatttttt caagaaaaac aaaagctata
     2221 agttacatgc agtagtgagt ggtaacagca ttaatattga gaaagtaaac gattctgtgt
     2281 tttcaaaaaa gttaatggga gatggagttg caattattcc taatagtaat gttgttgtag
     2341 ctccttgtaa tggtaaagta actgtgctta cagagtcaaa gcatgcattt ggtatggtct
     2401 cagatgaggg tgttgaaata ttagtccaca taggaattga tacagttaat ttacagggtg
     2461 aggggtttaa aaatgaagtg tctcaaggag atactgttaa aaaaggtagt ccaataataa
     2521 gctttgaaag agaaaaaata aattctcagg gaattgattg cacaacaata atggttgtat
     2581 taaatcatag cgaattttca gaaataaatt gcatggtaga aaatgaagtt gttgcaggga
     2641 aagatactgt tattgaaatt atgaaataat aggaagggat taattcaata tgagagtagt
     2701 aaaaatcttt aataataatg cattatctac agtaactaat gataggaaag aagctatttt
     2761 actaggtctt ggtataggtt ttaataaacg tccaggagat aaagttaatg aagataaaat
     2821 agaaaagatt tattatgttc aagaccacat gcaaacaaaa tttttagaat tattaaaaaa
     2881 tgttagtcca gaagtgatgg atgcttcaca acaaattata agtcttatac caggaaatga
     2941 ggggaaattt aataataaag ggatattatc tttagtggaa catattagtt ttgcaataga
     3001 tagaatgaaa aataatgttt ttttacctaa tttaatgctt ttggatataa agatgatgta
     3061 ttcaaaagag tttgaattag gagtaaaagc tttagaaatt atatacagag tatgtcatat
     3121 ccatttgcct gaagatgaag caggttacat agctttacat tttgtcaatt tacaatcaaa
     3181 tgataatttg gcatatgata ctttaaaatt tgtaaaaggt agtattgatt tgattaaaga
     3241 atgttatgga ttagaattag acgaatcgag tttgagtaca ttacgtttta gaactcatct
     3301 taaatttctt gctcaaagaa tttttaagaa tgaaatttgt caggatgata aaatgattga
     3361 aatgtatgat tatttaatta acaatcatcc taaaaataaa gaatatttag aaaagttaaa
     3421 tgtatatatt gaaaaggagt ttagatataa attagataaa cctgaaaaaa tttatctact
     3481 actacaccta acaaaaatct tataagaatg agattaatta gtaatagaaa tatgtataat
     3541 tttaaggggg gataagattg aaagagaaac tacttggagg actggaaaca tttgcaaagg
     3601 caatggttca gccattaatg tatttatcaa gtgcagggat attaatggtg gtaggtgtac
     3661 tacttacaaa tgatatgtta ttaggagtac ttccattttt aaaatgggaa ccaattcaaa
     3721 tatttggaag cttaatttat aattgtataa tggctattat taataatctt gctgttatat
     3781 ttgctatagg tattcctgct gcactagcaa aaaaagagaa gcacaaagca gcgttaatag
     3841 gatttatgag ttatataata taccttacag cttctaacac tatgttaaca tcactaggtc
     3901 aactagcaaa agaaagcaag atgcttggac tgatagggac tggacaagct actattttag
     3961 gtattcaagt tgtagacaca agtgtatttg gtggaattat tttagggtgt atagctggat
     4021 atgtatttaa tcgtacaagc aacaaaacat ttaaaggagc tttacagata tactcgggag
     4081 caaattttag ttttatatgc atgttctttg tatccatttt aattggagtt gtgagtaact
     4141 ttatatggcc attagctcaa caagaaatag cttctcttgc aaatgtcatc aaagatacag
     4201 gtacatttgg tttattctta tatggttttt tagagagaat attagtacca actggacttc
     4261 atcatttaat ctatacacca ttccagtttt ctgatttagg aggagtgctt aaagttggtg
     4321 cagatacagt ttcaggagcc tatgctatag ttatgacaga aatgaattta ccagttgagc
     4381 gtttctcaga tagtatatac tacatggcaa ctggatttac aaagatgttt ggttatatag
     4441 gtataggagc tgcctttatt tatactgcat ataagcaaaa taagaagaag gttaaggcaa
     4501 cggttattcc gctaataatt acagcagtat tagcctctgt aacggaacca ttagacttta
     4561 tgtttgtatt ctgtgcacca cttttattct ttgtacatgc agttatagct gggcttttta
     4621 tagctttatt aaaaatattt gatgttactg cactatgtgg aggtaactta ttagtatcaa
     4681 ttataatgaa tactacagta ggtgtagaaa aaacacactg gccagtaatg atgatattag
     4741 gacttgcaca aattattgta tattttgtag tatttagttt tttaattaag aaatttaatt
     4801 ataaaactcc aggtcgtgaa gatgagagta cagctacagg cgaagaacaa tcaaaacaac
     4861 ttaatttaga tgcaggtata gaaaatataa ttgatgggtt aggcggaaaa gaaaatatta
     4921 atacagtaga aaattgcatc actagattac gtgttaatgt aaaagatgaa agtaagatta
     4981 atgaagatat aattaattta acgccaaata gcgggattgt aaggaaagga aaagatatac
     5041 aaataatttt tggacttcat gtgcatgagg taagacgtgc agtagaagat tttttagaag
     5101 cttattaaag atatatatat ttatatagca agggtaaata acagctttaa gtgctgttta
     5161 ctatttatta aagatacata taatttatag aggaggaata attattatga ttattactat
     5221 tgttggtgga ggaagcacat ttactccagg tattgttaag tcaattgcat taagagaaaa
     5281 agagttagag cttgatgaaa ttcgcttatt tgatattgat aaagaacgtc aagataaagt
     5341 agctgtagtt gtcaactgga tactaaaaga agatgttaaa acagatgtta aactaactgt
     5401 aaccaattgt gaaaaagaag cttatacaga tgctacattt atatttgcac aaatgcgtgt
     5461 tggtaaatat gaaatgcgtg agcaggatga aaaaattcca ttgaaatatg gttgtgtagg
     5521 tcaagaaact tgtggctgtg gggggatggc atatggattg cgtacaatac aaccaatgat
     5581 aaaggttatt gatgatgtag agaaatatgc taaaaaagat tattggatat taaattactc
     5641 taatcctgct tcaattgttt ctgaagcttg tcgtaagtta agaccaaatg caaggattat
     5701 aaatatctgt gatatgccaa tagctattat agatatggta gcggggtcat taaatatcaa
     5761 tgacgttcat aatatacgct atgattattt tggtctaaat cattttggct ggtttacttc
     5821 aatagattat aaacatagag acttaatgga agagattaag gaatacataa aagaaaataa
     5881 aatattactg ccaaaatctt atatagaaaa cttaaataaa agtcgtagta caggaaatcg
     5941 ccatgctgta agtagctgga tacatgtttg ggaatctgtt tatacaatgt tagaaagctt
     6001 cccaaaatat ctgccaaaca cttatttaac atactatctt ttaagtaaag aaacagtgga
     6061 acattcaaac ataaatcgta ctcgtgcaaa tgaggttatg gaaagccgtg aaaaagactt
     6121 atttgatggt gtgagacatt acttagaaac tggagaaatt tctgaaaaag cattttatgc
     6181 aggttcacat ggagattgga ttagtgattt ggcagtttca attaaaaatg atacacgtag
     6241 tcgtttttta gttataactg aaaatcgtgg agctatacca aacatgccat atgatgcaat
     6301 ggtagaaatt tcagcatata ttggtaaaga tggtccagaa gttattgctc gaggtccaat
     6361 acctctattc caacaaggat taatgatgca acaacttaac agtgaaaaat tgatagtaga
     6421 agcttatata gagaatagtt atgaaaaagc tttacaagca ttcacattaa ataaaactat
     6481 tccaagtatg aatgttgcta aagaaatttt ggatgaaatg atagaagtta ataaggaata
     6541 ctggccagaa ttaagatagt aggataaaag aaagggtttt tatttaataa ttagatatgt
     6601 tttcagagtc ttttatcaaa atgtattcac aaacatatgg caatatatta aaataaagag
     6661 tgatattttt agaagaatgg ggtgatgcaa tgaacattat aaataaaatt ttttggcaag
     6721 aagataggat tggcatgatt acaaataatt tagaggcaga tacacatagc cattgcatgt
     6781 tacaactgtt cttagggata gaagacagca ttgaaataac agtcaatgaa aaattagtta
     6841 aatgtaattg tatcatagta gataaaaata tctctcattc attttcagca agaaaaaaag
     6901 tttattactc tgctattatt gaacctacat ctatatatgc agaacagctt acctcaaaaa
     6961 tgaatgattt tggctactgg atatgtgaca atgatggatt agagaaatta cgtcaacaag
     7021 gaactttttt gatatataac tctagtaaag aacaatattt aagatttatg gaaatgctta
     7081 ataactattt gaatatacct ataactctca aacattatga tgacagaatt actgaattgc
     7141 tcaatttatt acgtacttgt aattgtgata atcatactat ttcaagtttt gcagataaag
     7201 tgtccctatc agcaagtagg ttatcacatc tgtttaaaga acaaataggg atacctttaa
     7261 aaagttatat cttatttcat caaatggagt gtgcttttag agaattactt tctggaaaaa
     7321 atgttacgga agcttctatg actgctggat tcgatactcc atcacacttt gcagggacag
     7381 ttaagcgaat gatgggaatg cccgtttctt tatctttaaa agatagcgag tttttgaaag
     7441 tatattaaat attctcgtta tataatgaag ttgtactaag aaaggggttt tattatgaat
     7501 aaagaatatt tagaagaaac aaaaatgtta aattataatg aaccacaact taagcttttg
     7561 gtatcatcaa agaaatggtt ggaattagat gattttcata aaataaaatc tatttatgag
     7621 tttgtacaga atgatatttt atttggatat aatacttctg atatgttaag cgcaacacag
     7681 gtattaaatg atggtatggg acaatgcaat acaaaagcta ctcttttaat ggcattatta
     7741 cgcgcagtga atatcccttg taggcttcat gcttttgatg taacaaaaga ttttcaacga
     7801 ggtgctacat ccaaattgat ttcactatta gcaccaaaat atattcttca tacttgggta
     7861 gaggtatttt atcaagatag gtggattgct ctggagggtg tcataactga taaaaaatat
     7921 ttagaagcaa ttcagaagaa attttttaat catgatggaa cgtttaaaaa gtatgcaata
     7981 gctacaaatg atttgaaaaa tacatctatt gattggaatg gtaaagatac ttttatccaa
     8041 aaagaagcta ttgtgtatga ttatggtatt tttccatcac cagatgtatt tttttctact
     8101 cattcacaac atatgtcaaa attaaaaaat ttcatatatg tacatcttat cagaaaaata
     8161 atgacaaaaa atgtatgtaa ggctagaaac aattatattg ataaaaacga atgagtgttt
     8221 aagaggtgac tatataggaa atatatagaa taattaaaac tgtactggtt aaaatgaata
     8281 aaaaggagtt atcacagagc aaacttaaat tgttcatagg ataactcctt ttttgtatta
     8341 acttctaata atttgatatc tttttataaa acatatagat acaattaaac cagtaaatgc
     8401 aagagtagct gctattgaaa atgcaacatc tattccatga atgttagaca acatgtttga
     8461 actcatatga gctttcttag tagcacttgt cattatagta ataagtatag cagaacctat
     8521 agctccagat atctgacgga gtgtgttatt aattgctgtg gcatgagata taagttctct
     8581 atctaatgtt ttaattcccc atgtagttaa tggcattaaa accattgaaa taccaatcat
     8641 acgaatacaa tacatcaatg acacatgaat taaagatgta tccctgccta aaaaagcgaa
     8701 ggataaagtt cctaaaaaca aaaatcctgt tcctaatatt gatagagcat gaggtccata
     8761 tttatctaaa tgacgtcctg caattggatt gagtacaacc atcaatatag caccaggtaa
     8821 cattaatgaa cctgaagata atgcagaaaa tcctcttaca gattgaacgt atagaggaat
     8881 cattaatgta gcagacatca ttgaggcata tacaatcata attaatattg tagaaataag
     8941 aaatggttta tttttaaaaa ctctcaattc taaaaaaggt tctttagatt ttaattgacg
     9001 caatgtaaat aaaattaatg acataattcc tattaatatt ggtaaatatg tggcaatatt
     9061 agtccaccca taattacctt gattagtaaa tccaattaat aaaccaccaa aacctagaga
     9121 tgatagtact attgaaggta tatctaattt taagttttga gtttccccga cattttttaa
     9181 tagaataaat gatagtataa catctaatat agcaataggg gaaagaaaca gaaataagct
     9241 atgccaacca aatgaatcta ctaaccatcc tgataaagtt ggacctattg ctggagcaaa
     9301 tcctaccgta ataccaacta aactcatagc tgcaccacgt ttttcctcag ggcataaata
     9361 gagtataata acttgtaaaa gtggcattag agaaccagaa cccattgcct gcaatacacg
     9421 agcaaccaac atagttgaaa aattattaga aaaaagtgca ataatacatc ctatagaaaa
     9481 gatagacatt gatgtaataa ataactttct agtactaaat ctatttatta aataagctgt
     9541 tgtgggtaca ataatacctg caataagtag ataaattgtt gttaaccact gaccaacatc
     9601 agcagtaatt ttaaaatctg acattatatt aggtaatgct gttgacaata gtgtttgact
     9661 aagagaactt ataaaagcac cagtcattac aattgctatt attaaatttc cttttttatt
     9721 gttactttgg gtttccataa tttataacgt ctcctttttt aattgatttt taatactgtt
     9781 caataaaagt acttttggtc ttaaagtgtc ttctttttct aaatatgcaa tcattgcatc
     9841 cataattaaa gaatcatatt gttgattttt tccctcgacg tataattttt ttactgcttc
     9901 tagtaattta ggattttcaa ggagagaagt atccgttttg attagctctt ctatatcttg
     9961 tatcgtttta aaaaatagcg atttattatg aagggaaaag taaacttcat cttcttgttt
    10021 atgtaaaaac attgattttt caattaaagg atttagatta tccatatttt ctacaactaa
    10081 atctagaatt tgtatagtaa atttggcaat aaagtataca tttatttctc ttttttgtac
    10141 atgaataatg tatataaatt gatgaattaa agcatctaat aagaaaacta aatcaaatat
    10201 gattttttct attttatcac catagttatc aaataagctt aatttgtatt cattcaataa
    10261 gttagctcgt gattttgttt gcaatttact tgtagcctta cgttgctctt cagaaaagtt
    10321 ttctcttatg taggtattta tagtagttct agaaaaaata tttttccata cagcaattat
    10381 ctttctgttt aacttttctg atgatggaat gtcattattt tcatttatgg aatgtataat
    10441 ttctaaaaac ttttcatcat aataattgat tatttcgcag ataaaacttt ccttagtttc
    10501 aaaatgcttg taaaaagtag ctttagacat tttacactct tttacaacat ccgatattga
    10561 agtattttga aaaccttttt ggtcaaaaat tttttgtgca atagttataa tgagcatttg
    10621 tttttctgtc actttattat ctcctttcat atgaaaacat taagtactat atagtactta
    10681 taagttttca tagatatatt atacatcatt ttgaaaaaaa atcaatatat gtactataat
    10741 atgaaatttt tgaaaaattt attgttattt ttactcgtat aaaaaaaaat taagagtttc
    10801 ttaagaattg taagaatttc ttaagaattg taagagtttc ttaagatttg tttaaagatg
    10861 ttgcaataag ttagaagttg caataaatta gtggtatgat tatagcacaa gaaaaaatgt
    10921 tttgaaaatt ttctgtatta ggttaaaatt acggaggtgt agtatatgaa aaagaaaatt
    10981 tgtgtagcaa tagtttttgc tctatttata gtttttgcaa aaatgtttat tgacagttct
    11041 agtgaagaaa gaaacgttat gaaaaatgaa ttaagtaaat ataggtcagg tgtaaatgta
    11101 ttatcagtaa acaatattat agtagcgaat aaaaaataca gtttacctga aaactattct
    11161 ccacaggaaa gtattgaagc aagagatgct ttttttaaaa tgaatgaaga tgctcaaaaa
    11221 tcaggattaa atttaaaagc ttttagtaca tatagaagtt atgaatatca agatagatta
    11281 tttaaatcat acataaaaga acatggggaa gaagaagcaa atagattttc agctaaacct
    11341 ggagaaagtg aacatcaaac tggacttgcc tttgatattg gaggagatga caagtcttgt
    11401 tgggcaaatc aaaagttcaa caatacaaaa gaagctaagt ggttgtatga aaatgcatat
    11461 aaatatggtt ttatacttag atatccagaa ggtaaagaag atataacagg atatatgtat
    11521 gaatcttggc actatagata tgttggaact gaacatagca aaaactttgc aatgaataat
    11581 ttgactttag aagaatattt acatataaat taagaaatgg tttgttaaaa ttaggttaaa
    11641 ataaaaaata acctagacta ttaattgtgt aaagtgatat aatgtaaata caatttaaga
    11701 taactactcg aaggggagta gcttacacga aagtggtaat taatcgtcaa gacagtagaa
    11761 atactcggtt aattagcaag ggattccttg atagcaagac cttcaaaata agcattaggt
    11821 atgtttgttt tggggtcttt ttttattcaa ggaattttta tttaaaaaaa tttaaatttc
    11881 aagtatacat aagagccccc aaacataaaa taaatttatt aaagaggagg gcaacaaatg
    11941 tcttacaaaa gtacttatca agaagtaatt aaaaatttag actcaagctt atctggtttg
    12001 agtaaagaaa gagcagaaga gctgttagaa aaaaatggag ctaatgaatt aaaagaagca
    12061 gataaggttc ctacttataa gcttttctta gaaagtttta aagacccatt agtaataatt
    12121 ctattgatag ctgctttagt acaaatattt ttaggtgaaa ccgtggagtc tataataatt
    12181 tttgcagtta ttattataaa ctctgttcta ggtgttgtac aaacaaaaaa agcagaaagt
    12241 tcattagaaa gtttgaaaaa tttatctgca ccaaacgcaa aggtaataag agacaataaa
    12301 aaaatgacaa ttccagcaaa aaatttagta gttggagata ttgtattctt agaagctggc
    12361 gattacatac cagcagatgg aagacttata gaagcacaat ccctaaaggt tgtagaaggt
    12421 atgttaactg gagaatcaga accagtttta aagcatacag aaaaaataag tgaagatgta
    12481 agtttaggtg accagaaaaa tatggtattt agtggcgctg tagttgttta tggtagaggt
    12541 tcttttgtag tgactggaac aggtatggat actgaaatgg gtaaaatagc tgggctctta
    12601 gaaagtgctg aaaataaact tacaccattg caacaaaaaa tagaggattt tagtaaaaaa
    12661 cttggtgtag gtattgtaat acttgctatt ttaatattta taattcaagt ggcaagaaat
    12721 tactttatgg ctggaaatgg agaaatgacg caaattattt tagattcatt catgttctct
    12781 gtagcagtag cagtggcagc tataccagaa gctctatcat caatagtcac tatagtgttg
    12841 gctgttggaa caaatagtat ggcaaaaaaa caagctataa taagaaaatt accagcagta
    12901 gaaactttag gttcaacaag tgtaatatgt actgataaaa ctggtactct tacacaaaat
    12961 aaaatgacag tagttgatta ttatatgtat ggaactaata atgatgagtt agataaagaa
    13021 aaagtaaatt attcttatca tgctaaatta ttgactatgg tttctacatt gtgtaatgac
    13081 tctcatataa ctagtgatgg taaagaagtt ggtgacccaa cagaagtagc tttaataaat
    13141 tattcaagta aaaatgattt agattatgat aaactaagaa gtaagtatat cagaataaat
    13201 gaaataccat ttgactcaga tagaaagctt atgtctactg taaatagtat ctatggagat
    13261 tatataatgt ttacaaaagg tgctccagat attgtgttta gtagatgtaa atatgctttg
    13321 aaaaatggtt caaaagtaga tataacagat gagataatag aagaatataa acataaaaat
    13381 gaagagtttt caaatagagc acttagagta ttagcttttg caataaaaga tgttccagaa
    13441 gaagatttta taccttcaat tgatgatgaa catgacatga cattggttgg aataatggca
    13501 atgatagacc ccccaagaga agaagtaatg gatgctgtaa aagaggcaaa gagtgctggt
    13561 ataaaaactg ttatgataac tggtgaccat aaaacaacag cagcagcaat tgcaaaagag
    13621 attggtataa tggaagaagg ggacttagct cttactggaa aagagcttga tgctttgagt
    13681 gaagaacaac tatatgaaaa actagaaaat ataagtgtat atgccagagt ctcaccagaa
    13741 aataaaattc gtatagttaa ggcatggcaa cataagaata atataacagc aatgactggt
    13801 gatggtgtta atgatgcacc tgccttgaaa caagcagata taggaatagg tatgggaagt
    13861 ggtactgatg tagcaaaaga cgcttctgct atggtgttag ttgatgataa ctttgcaagt
    13921 atagtaaacg ctgttgaagt tggtagaaca gtatacagta atataataaa atcaattact
    13981 tatctatttg caggaaactt aggagctata gtatccatat tatttgcggt atttgcaggt
    14041 tggtcaaatc catttacagc tttacaactt ttatttataa acttagtaaa tgactcttta
    14101 ccagcaattg cattagggtt tgaaaagtca gaaaaaaata taatgaataa acctcctaga
    14161 catccagatg aaagtatact agcaggagga acattgaagg cagttgctat aagaggttgt
    14221 ataataggtg tagttacaat aattgcacaa tatataggat tacaagtatc tccagaactt
    14281 ggagtagcaa tggcattttc aacattgata ttagctagaa taatgcaaac attacctgca
    14341 cgttctaata atcaaacagt aataaaatta ggttttttca gtaataaata cgttatagga
    14401 gcagttttag tctgtcttgg gctttactct ttaacactaa taccagtgct aagaggaata
    14461 ttctctatac caacaacatt tggattgtac cagtttagca tttgcttagg tttagcatta
    14521 atttcatcaa tattaatgga acttaacaaa ttaataaata gaagtgaagt aaaataatca
    14581 aaaaattaaa gggcatattg attttaacaa tatgcctttt ataaatagtc taatttattc
    14641 tatattatcg attatttgga atgcttttat aactttaggt aattcttttt cttcaggcat
    14701 tctaaatctt atgaaatttt tatcaagtcc agtgaaatca cttccagata taactcttat
    14761 ttttaatttt gcaaattctt gttgtaaatc tatatccttg ttcttatgtt caactgtcat
    14821 tattgataca gtatcactag tttcagctat attaaggttt ttccatggaa ttaataattg
    14881 attttttata tcttttgttt tttcttttaa ttcttctata aattgaacat catctaataa
    14941 atttgctgca atactacgag atatttcaga cacttcgtaa ggagtagaaa tctttttaat
    15001 agggctaaca agttgttctg gaagaactgc atatcctgct cttagtcctg ctaagccaaa
    15061 tccttttgaa aatgttttta aagcaattac attatcataa ttatttagaa gcttgacagc
    15121 tgaattttct tttggcatgt attcaccata agcttcatct accataactg ctatatcata
    15181 tttagctgct tcctttacta tattttcaat tgatgataaa ggtataattt gtcctgtagg
    15241 attgtttgga ttgtctatgt aaattacttt atattctgga tttatttttt caataaattc
    15301 tttttcatta aacttgaaat tatcctcttt ctttaaaaga acataatcat aagttgcacc
    15361 gtgcattttt atatctgtct catattctga gaactgaggt gaatatccta aaactttatc
    15421 tcccttttca ataaatagtc tatttaaaag gtaaataaca tgtatagaac catctgccaa
    15481 acaaattctg tcagtgtcta atgttataaa gtctttccaa taatctacta tattatcttt
    15541 taaagcaatt gaatgaggat atcctcttat catatcaaat tttaaagcat taaaagcttc
    15601 tacagctttg tttgagtaag acactgtatt tataccttca ccacagtcta tctcgaatac
    15661 atcttctgaa tgtccagatt ctattgcata actttttttc aactgcattt caatagcttt
    15721 gttaattttt agtttcatac tttatcactc cttatttagt caataataaa tgttaaaaaa
    15781 gttgctagat ttaaaatatt atgaaaatat ataatagtaa aaaa
//
LOCUS       DAEPXF010000085        15712 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain HB27
            SAMN13376389-rid12479423.denovo.089, whole genome shotgun sequence.
ACCESSION   DAEPXF010000085 DAEPXF010000000
VERSION     DAEPXF010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13376389
            Sequence Read Archive: SRR10518432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 15712)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15712)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001069624.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001069624.1
            Genome Coverage       :: 315x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/15/2021 01:54:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,165
            CDSs (total)                      :: 4,076
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 89
            rRNAs                             :: 10, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 10 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 19 of 69
            Pseudo Genes (incomplete)         :: 44 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 10 of 69
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15712
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13376389-rid12479423.denovo.089"
                     /strain="HB27"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="China: Hebei"
                     /collection_date="2017"
     gene            22..798
                     /locus_tag="KRL67_002297"
     CDS             22..798
                     /locus_tag="KRL67_002297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease domain-containing protein"
                     /protein_id="HBH1523171.1"
                     /translation="MEYIIFDLEFNQGFDKKLNKTVSNEKCPFEIIQIGAIKLDSKFN
                     IIDTFNSYVKPTIYKDIHPFISRMTNIKNSDFNDSPTFPEVYNNFIKFISSQDPILCV
                     WGAGDLKELYRNINYHKLPSNSLPKSYINIQQHASKYFNNPAGKSIGLQNAISILELD
                     EKMSYHNALNDAYYTAKVFIKIYNPSIIPDIYLYTSIKPKTIRYSNKKRVDYDKLFDE
                     FRKILNRELTKDEKKIINLAYNMGKTNQFTLENVKQRKNK"
     gene            complement(890..1642)
                     /gene="xth"
                     /locus_tag="KRL67_002298"
     CDS             complement(890..1642)
                     /gene="xth"
                     /locus_tag="KRL67_002298"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HBH1523172.1"
                     /translation="MKFISWNVNGIRACVGKGFLDFFKEVDADVFCLQETKLQEGQIE
                     LDLPGYFQYWNYAERKGYSGTAIFTKKEPLKVMYGINIEEHDKEGRVITLEFEDFYFV
                     TVYTPNSQSELKRLEYRTRWEDDFIDYLTKLDNHKPVIVCGDMNVAHKEIDLKNPKNN
                     MKNAGFTKEEREKFSKLLDSGFIDTYRYFNPDKEGVYSWWSYRFNARKNNAGWRIDYF
                     CASKKLEDRLVSADIHTEVLGSDHCPVELEIK"
     gene            complement(1795..2745)
                     /gene="galT"
                     /locus_tag="KRL67_002299"
     CDS             complement(1795..2745)
                     /gene="galT"
                     /locus_tag="KRL67_002299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-phosphate uridylyltransferase"
                     /protein_id="HBH1523173.1"
                     /translation="MKELRIDPITNDVVIFATDRLKRPLDTADIPNEDEETNEYDEEC
                     PFCKGNEAYATDALFEIESEEGWLVKSVYNKFPIIDDMARDVYGVHEVMIESNKHNRS
                     FYNMSQKEFEDVFFMYRNRFRDLSKDDKVEYVSIFKNFLRKAGASLMHPHAQILSMSF
                     IPPEITNELLVSKEYYDSNKSSLYDDLIENEISLNRRVVYNGEAFLVLIPYATKYSGE
                     VRIIFKDKIEFGELNDNNIQELSTIFEKLFKKLYNINGYMPFNLCIHTHPTKIETKSY
                     FNVHMHIIPRKYNFGGFELGTNMYVSSMSPEELTKKLKID"
     gene            3130..4326
                     /locus_tag="KRL67_002300"
     CDS             3130..4326
                     /locus_tag="KRL67_002300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HBH1523174.1"
                     /translation="MNYSNRVSAMQASPIRKLVPFAQAAKDKGIKVYHLNIGQPDIKT
                     PKGFFDAVKNFDSEVLEYATSEGIPELLEALQNYYKTYDMNFEKDELLVTNGGSEALL
                     FTMMAVCDPGDNLLVPEPFYTNYNGFGQSVNVEVNAVTTKAENGFHLPSKEEILSKVD
                     DKTKAIILSNPGNPTGAIYTKEELNILAEIAKEKDLWIIADEVYREFVYDGLEYTSCG
                     NLEGVQDRVIIIDSVSKRYSACGARIGSIACKNKGLIAQILKLCQGRLCVPTLEQIGA
                     VELYKTPVSYFKEVNEEYKKRRDVLYNELMKVEGVICKKPTGAFYIVAKLPVENAEDF
                     TIWMLKEFNKDNETVMVCPAEGFYATPGLGRDEIRLAYILNEKDLHRAATLLKEGLEQ
                     YVALKK"
     gene            complement(4417..5778)
                     /locus_tag="KRL67_002301"
     CDS             complement(4417..5778)
                     /locus_tag="KRL67_002301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-alpha-glucosidase"
                     /protein_id="HBH1523175.1"
                     /translation="MKKRYNIAIVGGGSTWTPGLLKSLCKMSDRFPLNKVTMLDVVEE
                     RQRLIGEFGKILFKEEYPKATLEYTTNPDEAFIDVDFVFVQMRTGGLKMRELDEKIPL
                     QFGLVGQETCGAGGFAYGLRSIGDMIEMVKNVRKYSPKAWILNYTNPAAIVAEALKRV
                     FPDDKRLLNMCDQPVNLLRSYGRLLGMDSRTFEPVYFGLNHFGWFTHLYDKNGEDLVP
                     KIKALVANSGFQPVDAEQRDKSWLDTYGMVKDMLEDVPDYLPNTYVQYYLYPDKKVAK
                     SNINCTRAREVMNGREKRVFEECKSVIEKGTSIGSNLVHNDAHGEFIVEVAEAIAHNK
                     QQIAIVITENNGAINNLPDDAMVEVAAILTKNGPRPLHVGNIPTFYKGLLEGQLAYEK
                     LAVDAYFEQSYEKAILSLTLNRTIISPTKARQVLDALIKVNKDYWPTLYKENEKESGI
                     SLN"
     gene            complement(5917..6669)
                     /locus_tag="KRL67_002302"
     CDS             complement(5917..6669)
                     /locus_tag="KRL67_002302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HBH1523176.1"
                     /translation="MNLIDKLDINRKKLSTNEISLLNYFLKNSEKINQKKIKDIANET
                     FNSTAYIVRFCQKLGFSGYSEFKNFLVFSYSNRNERNKEFTKSQSDIFTDVMDTQNLI
                     NVENIDMCLNEIHNSKHIYFFGVGSSRLVCTEMSQRFSAIGVNVKYYDDSTLMYLAAS
                     NITKDDLVIAVSMSGETAQVIKACNIAKTNQVKVISLTNISVNSLSNIADIKLFVSSP
                     QYNFNSVNYTSRVPALSLMEYMFHKYIEKYNR"
     gene            complement(6817..8394)
                     /locus_tag="KRL67_002303"
     CDS             complement(6817..8394)
                     /locus_tag="KRL67_002303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HBH1523177.1"
                     /translation="MKQKMKGFFLQLGKSFLLPIALISAAGIFLGISSAFSNPNIVSD
                     IPFLANNFIQGILGFTKAITGVLFGNLAIFFAISISVGLAKEEKAVSAFSGFVGYLVL
                     HTSVNYMLGCRGILAEPDMMREMGQAVTLGIQTLELGVFGGIVVGIITASLHNKYYNI
                     KLPDYLGFFGGTRFVPIITTVVFSILGIIIPYIWPYVNTIIQSVGIGIASLGYLGTFA
                     FGFLERLLIPFGLHHILNAMFRFTEVGGSLVVGGQEVFGALNIFLAQLSDPKTISFST
                     EATRFLAQGKIPIMVFGLPAAAYAMYKTAKPENRSKVKGILLAGALATFVTGITEPLE
                     FSFLFVSPILFVIHAFLSGISFMMMHILGVAIGNTQGGIIDLVVFGVMQPNTKWIYSI
                     MLGLVYALVYYNVFKILILRFNLQTPGREEIADINFDLEPLSNKVEIAQSGETTKRAL
                     SIIKYLGGKDNIEDITNCITRLRVVVKDMSKVDENALKKYTGAMGVIKVSDKDIQIIY
                     GPSVSQVRDEVSFAMSI"
     gene            complement(8798..9250)
                     /locus_tag="KRL67_002304"
     CDS             complement(8798..9250)
                     /locus_tag="KRL67_002304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HBH1523178.1"
                     /translation="MNYIVKDTISFLVKTFPKIYSSLYLEDLKKFAPDYNVNKTQLRA
                     LVFIKNYGVISMTDLCSKLNIEKGSLTSMVDDLTDKKYVTRKRDLVDRRKYLIDITQE
                     GDKIAADFMDKLSDGLGEKLSKLTEEDRRKYLEAINTLQYILNKEEFR"
     gene            complement(9561..11006)
                     /locus_tag="KRL67_002305"
     CDS             complement(9561..11006)
                     /locus_tag="KRL67_002305"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HBH1523179.1"
                     /translation="MIDNKYTTYSNPLTDRYCSKDMSYIFSPQFKFSTWRRLWVALAE
                     GEHELGINITEEQLDELRANIDNINFDEARKIEKEVRHDVMSHVKAYGLQCEKAKGII
                     HLGATSAYVGDNTDVIQMNEALKLLRVKLINLINNLKEFALKYKDMPALGFTHFQAAQ
                     LTTVGKRACLWAQDLILDLEDLNYRIDNMKLRGVKGTTGTQASFLSLFEGDHEKVKEL
                     DKKICKKMGYHSSYAVSGQTYTRKLDYQVISILSGIAQSMHKMTNDIRLLQSLKELEE
                     PFEKNQIGSSAMAYKRNPMRSERIASLSKYIISESINPAMVQATQWLERTLDDSANKR
                     LAIPQAFMAADAILEIGINVTDGLVVYENMINKRVNEELPFMATETILMEAVKRGGDR
                     QELHEIIREYSMKAAYRVKHEGKDNNLIELIMNDDSFKMSKEEILSIMDPKNFIGRAP
                     EQVVEFVNEVVEPAIKDYKEDLGKVDVDLRV"
     gene            complement(11150..12058)
                     /locus_tag="KRL67_002306"
     CDS             complement(11150..12058)
                     /locus_tag="KRL67_002306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HBH1523180.1"
                     /translation="MYSTKILDDYEYETFNVFSTKLNPNTKHDYLSKVILFKEFLKGK
                     ELIYATKEDCKNFVDYIQTKYAKSTCEKIYSYLHSFYNFLKKEGYIDINPFRYVEKPT
                     VTRIKTKDDVLSIQEINKLIGILPKLNIRDRVIIVCLVTTGCLLNELVSLKWKDLMVD
                     ENDNSYVRLGKGRKERVVKLHPYFFKLLEDYRNYSGLPEVIIPSDDFIFTTQKSNSIT
                     DRNVRLIVKKALDLAGLSQYSARDFRHSFAAISLRLGADESDVKNQLGWSDKYYAIRY
                     KYVLNFVDSEIVDYLIEKDNLDINKK"
     gene            12315..13139
                     /locus_tag="KRL67_002307"
     CDS             12315..13139
                     /locus_tag="KRL67_002307"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HBH1523181.1"
                     /translation="METFSLLEIFKAVILGIVQGITEWLPVSSTGHMILVDEFIKLNF
                     SNTFISTFLVVIQFGSILAVLVIFFRKLNPFDSAKNIKQKKETVRLWLKVIIAVIPSG
                     VIGILFEDDIDRLFFNSTVVAIALIVYGIIMIGLEKRNKRPKYKDFSQVTYKLALCIG
                     LFQCLALIPGTSRSGSTIIGAVLLGTSRYVAAEFSFFLAIPTMLGASALKLLKAGFGF
                     TGFEWLILGVGSVVAFVVSIVVIKFFMDYIKKHDFKVFGYYRIVLGIVVLAYFFLL"
     gene            13179..13709
                     /gene="lepB"
                     /locus_tag="KRL67_002308"
     CDS             13179..13709
                     /gene="lepB"
                     /locus_tag="KRL67_002308"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HBH1523182.1"
                     /translation="MSVKKEIFDWIKSIAMAIVLAFVILQFIRPSIVSGESMYPTLDD
                     KDYLILNRISYKVGKPEKGDIVVFKTNLVDGETGKKKDLIKRVIATEGDRIKISNSKV
                     YVNGKLLNEPYIHNNYTSGDIDTVVPKGKLFAMGDNRENSNDSRFPDVGMVDEDEILG
                     KVMVRLLPLDNIGKVD"
     gene            complement(13791..15572)
                     /locus_tag="KRL67_002309"
     CDS             complement(13791..15572)
                     /locus_tag="KRL67_002309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HBH1523183.1"
                     /translation="MSQYININSQLDALKLKEFEKIFYENINEDVKVVPKVTPFKGIN
                     TDLLYVKDGRILFIKFMDTTEDIFFILEEELLEVMNEEYELLKLKMGQKNRNISYNYV
                     YIMPYVEVEETYEFEEFVNNNIIDKNKLQDIMNKGSLEEYLNDENDEINLNLFLLDIC
                     SEYYIINDKLHLNEKFKKISFYNDDYKYTATMMEEVQIKDVISIKYGNTLIEGGSGIG
                     KTAIMLSRAIKLAKVYPHHKLVIFTHTKQLCNELRERIELLYKDNNNLEVHTFSSFIF
                     KLAKKFNLIVDYNMLKNDYEKTFNNLVKQAQNIIKNKNMFKAIFIDEAESFLEYEIDF
                     IREFLYKTKFIFNVCSCNSLNISNRLNIFKKLYNGIEFDDKIILSKNYRQAKEIVDFT
                     NKFSNNSNSYINELRPNTEFSTFFYTKALRGGNKSVDIIKVSDLDDQISSVIWEIEYL
                     ISKKGLDYSEVAIVYPYNKKKLKSGKTIYFQYMLKKALEEAKIPYICAEDNLTNISKK
                     VGTTIANIYAIKNLEYKAVIVCELEMLYNQTINDTEQDYQVNDFVGDLNKVYLAMSRA
                     TDYLSIVTTFNEEASDIIRLITESKDI"
BASE COUNT         5302 a         2469 c         1873 g         6068 t
ORIGIN      
        1 aaaatttagg agataaactt tatggaatac ataattttcg atttggaatt taaccaagga
       61 tttgataaaa aactcaataa aactgtatcc aatgaaaagt gtccttttga aattatacaa
      121 ataggagcaa ttaaattgga ttctaaattc aatataatag atacgtttaa tagctatgta
      181 aaacctacaa tttataaaga catacatccc tttattagtc gtatgactaa tattaaaaat
      241 tctgatttta atgattcgcc tactttccct gaggtctaca acaattttat aaaatttata
      301 tcatcacaag accctatatt atgtgtttgg ggagctggag atttaaagga attatacagg
      361 aatatcaact accacaagct accatcaaat tccttaccta aatcttatat taatatacaa
      421 caacatgcat ctaaatattt caataatcca gcaggaaaaa gcattggctt acaaaatgct
      481 atttctatat tggaacttga tgaaaaaatg tcttatcaca atgctttaaa tgatgcatat
      541 tatactgcta aagtttttat taagatatac aatccaagta taatcccaga tatatatctt
      601 tatacgagca tcaaacctaa aactataaga tattctaata aaaaaagagt tgattatgat
      661 aagttatttg atgaatttag gaaaatttta aatcgtgaat taactaaaga cgagaaaaaa
      721 atcattaact tagcttataa tatggggaaa acaaatcaat ttacattaga aaatgttaag
      781 caaagaaaaa acaaatagag tttatttaga taaaaaatag gcatgtagat taaaaaacta
      841 gttaacttta aatttatatc tacagcccaa tttttatcgt aattaatttt tattttatct
      901 ctaattcaac tggacaatgg tctgaaccta atacttcagt gtgtatatcc gcacttacca
      961 atctatcttc taattttttg gatgcacaaa aataatctat tctccaccct gcattatttt
     1021 ttcttgcatt aaatctatat gaccaccaac tatagacacc ttctttatca ggattaaaat
     1081 atctatatgt gtctataaat ccactgtcta ataatttaga aaacttctct ctttcttctt
     1141 tagtaaaacc tgcatttttc atattatttt taggattttt taaatctatt tctttatgtg
     1201 caacgttcat atctccacac acaattacag gcttatgatt atctagtttg gttaaataat
     1261 ctataaagtc gtcttcccat ctagttctat attctagtct ctttaactca gattgagaat
     1321 ttggcgtata tactgtaaca aagtaaaaat cttcaaactc tagagttatc actctaccct
     1381 ctttgtcatg ttcttctata tttattccat acattacttt taaaggctct ttttttgtaa
     1441 atatagcagt acctgaataa ccctttctct cagcataatt ccaatattga aaatatcctg
     1501 gtaaatctaa ctctatttgc ccttcttgca atttagtttc ttgtaaacaa aatacatctg
     1561 catctacttc tttgaaaaag tctaaaaatc ctttcccaac acaagctctt attccattaa
     1621 cattccatga tataaatttc atacttatac tcctcatcta aacattgata cactccccat
     1681 agctaaagct gggggctttt caacaagttt ataaattctt aatttacttt tattttaaca
     1741 atactataat ttcatctaaa attactattt acttaatacc taaaaatata tgttttaatc
     1801 aatttttaac ttttttgtta actcttcagg gctcattgaa gaaacataca tatttgttcc
     1861 aagttcaaat cctccaaaat tatattttct tgggattatg tgcatatgaa cattaaaata
     1921 cgattttgtt tcaatctttg ttggatgagt gtgaatacat aagttaaatg gcatatatcc
     1981 atttatattg tataacttct taaacaactt ttcaaatatt gtagaaagtt cctgtatatt
     2041 attatcattt aattcaccaa actctatttt atctttaaat attattctta cttctccact
     2101 atacttggtt gcatacggta taagtaccaa aaaagcttct ccattataaa caactcttct
     2161 atttaaactt atttcatttt ctattaaatc atcatacaaa gaacttttat tgctgtcata
     2221 atactccttt gaaaccaaca gttcattcgt aatttcaggt ggaataaaag acatagacaa
     2281 tatttgagca tgtggatgca tcaaagatgc tcctgccttt ctcaagaagt ttttgaagat
     2341 actaacatat tctactttat cgtctttcga caaatctcta aatctatttc tatacataaa
     2401 aaaaacatct tcaaattctt tttgactcat gttataaaaa ctacgattat gtttattgct
     2461 ctctatcatt acttcatgaa caccatatac atctcttgcc atatcatcaa ttataggaaa
     2521 tttattgtag acagatttta caagccatcc ttcttcactt tctatttcaa ataatgcatc
     2581 agttgcataa gcttcatttc ctttgcaaaa tgggcattct tcatcatatt cattagtttc
     2641 ttcatcttca tttgggatat ctgctgtatc taaaggtctt tttaacctat ctgtagcaaa
     2701 tataacaaca tcattggtaa ttggatctat tcttaattct ttcataatta acacctctta
     2761 gttaatagcc tttaatataa ttatatgata tttatcgtat ctgttaaata aatttataat
     2821 attttttata attttgcata tttattatta tatattttgt ctatagctga aagattataa
     2881 ctaaaaaatc actatcagta tatcagaata ttatcttact ataaattaat ttatgaatac
     2941 ataatttagt agcataaaca tgggtatttt actataattt gttaaaagtt tattttaaag
     3001 ctatattaat tttttttgat gatttttacc cataagtatg ttattattat cttgtaaaga
     3061 tgtcttgtat tcaaacatac aatttacata atacaatata cgtatttaaa ttaaataggg
     3121 ggtattaaaa tgaattattc aaacagagtt agtgcaatgc aagcctctcc aatcagaaaa
     3181 cttgttccat ttgcacaagc agcaaaagat aaaggaatca aagtatatca cttaaacata
     3241 ggacaaccag acataaaaac tcctaaagga ttttttgatg ctgttaaaaa ctttgatagc
     3301 gaagtcttag aatatgctac atcagaaggt attccagaat tattagaagc acttcaaaat
     3361 tactataaaa cttacgatat gaattttgaa aaagatgaat tattagtaac taatggtggt
     3421 agtgaagctt tacttttcac tatgatggca gtttgtgacc caggagataa tctattagtt
     3481 ccagagccat tctatacaaa ctacaatgga tttggtcaat ctgtaaacgt agaagttaat
     3541 gctgttacaa caaaggcaga aaacggtttc caccttccat ctaaagaaga aatattgtct
     3601 aaagtagatg ataaaactaa ggctataatt ctttctaacc caggaaatcc aactggtgca
     3661 atttatacaa aagaagaact taatatccta gctgaaatag ctaaagaaaa agatttatgg
     3721 ataatagcag acgaagttta tagagaattt gtttatgatg ggcttgagta tacaagctgt
     3781 ggtaacttag agggtgtaca agatagagtt ataataatag acagtgtttc taagagatac
     3841 agtgcttgtg gtgctagaat tggttctatc gcttgtaaaa ataaaggtct tatcgctcaa
     3901 atacttaaat tatgtcaagg aagactttgt gttcctacat tagagcaaat aggagctgtt
     3961 gagttataca aaactcctgt ttcttacttc aaagaagtta atgaagagta caaaaaaaga
     4021 agagatgtat tatacaatga attaatgaag gttgaaggtg ttatatgtaa aaaaccaact
     4081 ggtgctttct atatagttgc taaattacca gttgaaaatg cagaagattt cacaatttgg
     4141 atgttaaaag aatttaacaa agataatgaa acagttatgg tttgccctgc tgaaggattc
     4201 tatgctactc caggtttagg acgtgatgaa ataagattag cttacatcct aaatgaaaaa
     4261 gaccttcata gagctgctac attattaaaa gaaggtttag agcaatatgt agctttaaaa
     4321 aaataatttt ttaaacttaa tttatataaa tttaatgata taaaatgtcc ccttttcaga
     4381 tattatttga aaaggggaca tttcttaatt aatatattaa ttaagactta tacctgactc
     4441 tttttcattt tctttatata gtgttggcca gtaatcttta ttcactttaa ttaaagcatc
     4501 taaaacttgt cttgccttag ttggagaaat tatagttcta tttaatgtta aagataaaat
     4561 agctttttca taagattgtt caaaatacgc atcaactgct aacttttcat aagctaattg
     4621 accttctaac agtcccttat aaaatgttgg tatattacct acatgtaaag gtctcggtcc
     4681 attctttgta agaatcgcag ctacttctac cattgcatca tcaggtaagt tattgatagc
     4741 cccattattt tctgttataa ctatagctat ttgctgtttg ttatgagcta ttgcttctgc
     4801 aacttcaact ataaattccc catgagcatc attatgcact aaattagaac ctatagatgt
     4861 tcctttttct ataacagatt tacattcttc aaatacacgc ttttctcttc cattcataac
     4921 ttctcttgct ctagtacaat ttatatttga tttagctact tttttatcag gatatagata
     4981 atactgtaca tatgtattag gtaagtaatc tggtacatct tccaacatat ctttaaccat
     5041 tccataagta tctagccaag atttatctct ttgttctgca tcaacaggtt ggaatccaga
     5101 gtttgctact aaagctttta tcttaggtac caaatcttca ccatttttat catacaaatg
     5161 tgtaaaccat ccgaagtgat ttaaaccaaa gtatacaggc tcaaatgttc ttgaatccat
     5221 acctaacaat cttccataag accttaacaa gttaactggt tggtcacaca tattaagcaa
     5281 tcttttatca tctggaaata ctctttttaa tgcttctgca acaatagcag caggatttgt
     5341 ataatttaat atccatgctt taggagagta ttttcttaca ttcttaacca tttctatcat
     5401 atcacctata gaccttaacc cataagcaaa tccaccagct ccacaagttt cttgaccaac
     5461 caatccaaat tgtagaggaa tcttttcatc taattctcgc atcttaagac caccagttct
     5521 catttgaaca aatacaaaat ctacatctat aaatgcttca tccgggtttg tggtatattc
     5581 aagtgttgct ttaggatatt cttctttaaa taatattttc ccaaattccc ctattaatct
     5641 ttgacgttct tccactacat ctaacatagt tactttattt agaggaaatc tatcactcat
     5701 tttacacaaa gactttaaaa gtcctggagt ccaagtactg cctcctccaa ctatagctat
     5761 attgtatctt tttttcatta ttatattaat cacccctgtc ttttattaac taacttgtaa
     5821 gatgattata atatataaag ttttctacat gaatactttt aggtaatcat taaaaatagt
     5881 tcaattttag tatttttttc aaaattgtaa tttttttcat ctattgtatt tttcaatgta
     5941 tttatgaaac atatattcca taagagaaag tgctggcact ctagatgtat agttaacact
     6001 attaaaatta tattgtggag aagatacaaa taattttata tcagctatat ttgataatga
     6061 attgacactt atatttgtca gagaaattac tttaacttga tttgtttttg ctatattaca
     6121 tgccttaata acctgagcag tctctccaga catagataca gcaattacaa ggtcatcttt
     6181 agtaatattt gatgcagcca aatacatgag agttgagtca tcatagtatt ttacatttac
     6241 acctatagct gaaaaacgtt gagacatttc agtacatact aatctagagg acccaacacc
     6301 aaaaaaatat atatgcttag aattatgtat ttcatttaaa cacatatcta tattctctac
     6361 atttattaaa ttctgtgtgt ccataacatc agtaaaaata tcactttgac ttttagtaaa
     6421 ctccttattt ctttcatttc tatttgaata tgaaaaaact aaaaagtttt taaattcact
     6481 atatccagaa aagccgagtt tctgacaaaa ccttactatg tatgcagtcg aattaaaagt
     6541 ttcatttgct atatctttaa tctttttttg atttattttt tcagaatttt ttagaaaata
     6601 gtttaaaaga gatatttcat ttgtagataa tttttttcga tttatatcta atttgtctat
     6661 taagttcatt tatacctcca taataatgat ttgtagttaa acaactttct catatttatt
     6721 atataaatat acactataca gcatttatct tatgtttatt atactacaaa aaagacaata
     6781 caaattataa attagtattg ccttttaata tttactttaa atagacattg caaaagaaac
     6841 ttcgtctctt acttgagata cagatggccc ataaattatt tggatatctt tatcagatac
     6901 ttttataact cccattgcac ctgtatattt ttttaaagca ttttcatcta ctttactcat
     6961 atctttaact actactctta agcgagttat acagtttgtt atatcttcta tattgtcctt
     7021 accacctaaa tatttaatta tggataatgc tctcttagta gtttctccac tttgagctat
     7081 ttctacttta tttgataaag gttctaaatc aaaatttata tccgctattt cttctcgtcc
     7141 tggagtttgt aagttaaatc taagtatcaa aatcttaaat acattataat aaactagtgc
     7201 atacactaaa ccaagcatta tagaataaat ccactttgta tttggttgca taactccaaa
     7261 tactactaaa tctattatgc caccttgagt atttcctatt gctactccca atatatgcat
     7321 catcataaat gatatacctg ataaaaatgc atgtataaca aataatattg gtgatacaaa
     7381 taagaatgaa aattccaatg gttccgtaat tcctgttaca aatgttgcta aagcaccagc
     7441 tagtaatata cctttaacct tcgacctatt ttcaggcttt gcagttttat acatggcata
     7501 tgctgctgca ggaagaccaa ataccattat aggtattttc ccttgagcta aaaatctagt
     7561 tgcttctgtt gaaaaactga ttgttttagg gtcacttagt tgcgctaaga atatgttcaa
     7621 tgctccaaat acttcttgcc ctcctacaac taaagaacct cctacttcag taaatctaaa
     7681 catagcattt aatatatgat gtagtccaaa tggtattaaa agtctttcaa gaaatccaaa
     7741 tgcaaatgtt cctaaatatc ctaaactagc tataccaata cctacactct gaataattgt
     7801 atttacatat ggccatatat atggtattat tatccccaaa atagagaata ctactgttgt
     7861 tattattgga acaaacctag ttccaccaaa aaatcctaaa taatctggca gttttatatt
     7921 gtagtattta ttatgtaatg atgctgttat tataccaact acaattccac caaatactcc
     7981 taattctaat gtttgtatgc ctaaagttac agcttgaccc atttctctca tcatgtcagg
     8041 ttcagcaaga atacctctac accctaacat ataatttaca ctagtatgta atactaaata
     8101 accaacaaaa ccagaaaatg cagaaactgc tttttcttct ttagcaagac caacagatat
     8161 tgatatagca aagaatattg ctaagtttcc aaaaagtact cctgttatag cttttgtaaa
     8221 acccaatatt ccttgaatga aattatttgc taaaaaaggt atatcactaa ctatattagg
     8281 atttgaaaat gctgaactta ttcctaaaaa tatacctgct gcagatatta atgctattgg
     8341 caataaaaat gattttccta actgcaaaaa gaaccctttc attttttgtt tcatttctat
     8401 ccctccattt ttaaattttt aaaacgtttt ctatgtcata attatatatt tttattttgt
     8461 ttttgaaact ttataatttg ttttaaaata tttttacata aatagtaact ttttcaaaac
     8521 tgtaaatttt ttcaaacaaa gacatagtaa cgttgtaaca tatataaatt taaaaagaga
     8581 ttagaaaata aaatcattga atataaaata gaaaaagata gatttcgcaa atagatatga
     8641 gtaggtactg acaaatactg aaatttataa gaaattataa aaaagtacat cgaaaaaatt
     8701 taagatgtac tttttagatt gtatttagat ttataattat atagctaaat gtataacaaa
     8761 gaattaatct taaaactatt aaaatttttg tatttttcta tctaaattct tctttgttca
     8821 atatatattg aagagtattt attgcctcta aatattttct tctatcttcc tcagtcaatt
     8881 tagaaagctt ttctcctaaa ccatcactca acttatccat aaaatcagca gctatcttat
     8941 ctccctcctg agtaatatca attagatatt ttcttctatc aactaaatct ctttttcttg
     9001 ttacatactt cttatcagtt aaatcatcaa ccatactagt taaactgcct ttttctatat
     9061 ttaatttaga gcataaatca gtcatactta ttactccata attttttatg aaaactaatg
     9121 ctcttaattg tgttttattt acattataat ctggtgcaaa tttttttagg tcttctaagt
     9181 acaggctaga ataaatctta ggaaatgttt ttactaaaaa acttatggta tcttttacta
     9241 tatagttcat ataatcacct cggttgtttt aaaatttatt aaattatcta aataacttta
     9301 tttcagttta cattaaaagt attgcaagta caatagtttt tgctgaatgt ttaaatttaa
     9361 ttagtaatct tatcattttt ttatttttat ttatattatt cttattaaat actatttata
     9421 tagctatttt attaaattta tggtatagta agtaaagaag aactcttcac agcggtgaag
     9481 agaagacgct agcccattaa aatggctatc ttatttataa ataagatagc cgtttttaat
     9541 tatattttat ctgtacaatt ctatactctt aggtctacat ctacttttcc taagtcttcc
     9601 ttataatctt ttatagcagg ttctacaact tcatttacaa attctacaac ttgttctgga
     9661 gctctaccta taaagttttt tgggtccatt atagatagta tttcttcctt actcatttta
     9721 aatgaatcat cattcattat aagttcaatt aagttattat ccttaccttc atgctttact
     9781 ctataggcag ctttcataga atattctctt attatttcat gtaactcctg tctatctcca
     9841 cctcttttta cagcttccat aagtattgtt tctgtagcca taaaaggaag ttcttcattt
     9901 acacgtttat ttatcatatt ttcatatact accaagccat cagttacatt tatccctatt
     9961 tcaagtatag catctgctgc cataaatgct tgaggtattg ccaatctctt attggctgag
    10021 tcatctaatg ttctctctaa ccattgagta gcttgtacca ttgcagggtt tatagattca
    10081 gagattatat acttagataa agatgctatt ctctcacttc tcattggatt cctcttataa
    10141 gccattgctg atgaacctat ttgatttttt tcaaatggct cttctaattc ttttaaactt
    10201 tgtaaaagtc ttatatcatt agtcatctta tgcatacttt gagctatgcc tgatagtatg
    10261 cttattactt gataatctaa ctttctagta tatgtctgtc cacttactgc ataggaagaa
    10321 tggtatccca tctttttaca aattttctta tctaactctt ttactttttc gtggtctcct
    10381 tcaaataagc ttaaaaaact tgcttgagta ccagtagtac ctttaactcc tcttaacttc
    10441 atattgtcta ttctatagtt taaatcttcc aaatcaagaa ttaaatcttg agcccataaa
    10501 caagctcttt ttcctactgt tgtaagttga gcagcttgga agtgtgtaaa tccaagagct
    10561 ggcatatctt tgtacttaag tgcaaattct tttaaattgt ttattaaatt tataagcttt
    10621 actcttagaa gttttaaagc ttcgttcatt tgtataacat ctgtattatc tccaacatat
    10681 gcagatgttg ctcctaaatg tattattcct ttggcttttt cacattgtaa accataagct
    10741 tttacgtgag acattacatc atgtcttact tctttttcta tttttctagc ttcatcaaaa
    10801 tttatattat ctatattagc tcttagctca tctaattgtt cttcagttat attgattcct
    10861 aactcatgtt caccttctgc aagtgcaacc cataaccttc tccatgtaga gaatttaaat
    10921 tgtggagaaa atatataact catgtccttg ctacaatatc tgtctgtaag tgggtttgag
    10981 taagtagtat atttattatc tatcattata ttagcacctt ccttaaatct tttgtcgtgt
    11041 gattactgtg tacacattag tacataaata attatatata attttacgaa ctataacaag
    11101 acttttttat tttttgttca catttatata tagtgttttt tatttattac tattttttat
    11161 ttatatctaa attatctttt tcaattaagt aatccactat ttcactatca acaaaattta
    11221 atacatactt atatctaatt gcatagtatt tatcactcca tccaagctga ttttttacat
    11281 cactttcatc tgccccaagc cttaaactta ttgctgcaaa tgaatgtcta aagtctcttg
    11341 ctgaatattg agaaagccca gctaaatcta atgctttttt gactataagt ctgacatttc
    11401 tatctgttat agaattactc ttttgagttg taaaaataaa atcatcacta ggtattatta
    11461 cctctggaag cccagaataa ttcctgtaat cttctaaaag tttaaaaaag tatggatgta
    11521 acttaactac tctttctttt cttcccttac ctaaccttac atatgagtta tcgttttcat
    11581 ctaccataag gtctttccac tttaagctaa ctaattcatt taataaacac ccagtagtaa
    11641 ctagacagac tattattact ctatctctta tatttaattt tggtaggata cctataagtt
    11701 tatttatttc ttgtatactt agaacatcat cttttgtctt tattcttgtt actgtgggtt
    11761 tttctacata tctaaatgga tttatatcta tgtatccttc tttttttaag aagttataaa
    11821 aactatgtaa ataactatat atcttttcac aagttgattt cgcatattta gtctgtatat
    11881 agtctacaaa atttttacaa tcttctttcg tagcataaat caattctttc ccttttaaaa
    11941 attctttaaa cagtataacc tttgataaat aatcgtgttt cgtgtttgga tttaattttg
    12001 tagaaaaaac attaaaagtt tcatactcat aatcatctag tatttttgta ctatacataa
    12061 taactccttt ccttttcata aaataaaata gctatacttt tactatacca tatatttccc
    12121 cctcaaatat accccttatt caatagataa cttttaagtt tattttgtta tattatgaag
    12181 gttttatgaa aatatcaatc ttccattgtg cactacacag tactatggta ttataagtta
    12241 gtaaaaggtg gcattttttt attaatctat gaataaaata tgctactata aatttgtatt
    12301 ggaggtattt tgatttggaa acatttagtt tgcttgaaat ttttaaggca gtcattttag
    12361 gcatagttca gggaataaca gaatggctac cagtaagtag tacagggcat atgatattag
    12421 ttgatgagtt tataaaattg aatttttcaa atacatttat aagtacattt ttagtcgtaa
    12481 ttcaatttgg ttcaatatta gctgtacttg ttatattttt tagaaagtta aatccttttg
    12541 acagtgcaaa aaatattaaa cagaaaaaag aaacagttcg tttatggctt aaagttataa
    12601 ttgcagttat accatctgga gttattggaa tactgtttga agatgatata gatagattat
    12661 tctttaattc aacagtagtt gctattgctc ttattgtcta tggtataata atgatagggc
    12721 ttgaaaagag aaataaaaga cctaagtata aagatttttc tcaagttaca tataaattag
    12781 ctctttgtat aggattattc cagtgtctag ctcttatacc aggtacatct aggtctggtt
    12841 ctactataat aggtgcagta ttacttggta cttctagata tgtagcagca gagttttcat
    12901 tcttcttagc catccctaca atgcttggag ctagtgcatt aaaacttcta aaagctggat
    12961 ttggatttac tggatttgaa tggcttattt taggtgttgg ttctgtagta gcatttgttg
    13021 tttcaattgt agttattaaa ttttttatgg actatatcaa aaaacatgac ttcaaagtat
    13081 ttggttatta tagaatagtc ttgggtatag tggttcttgc ttatttcttt ctattataat
    13141 gtattctaat ttaataacaa atgataagga gaaaagttat gagtgttaaa aaagaaatat
    13201 ttgattggat taagtcaata gctatggcta ttgtacttgc atttgtaatt ctacaattta
    13261 taagaccttc tattgtaagt ggagaatcaa tgtatcctac tttagatgat aaagattatc
    13321 tgattttaaa taggatatca tacaaggttg gtaaacctga aaaaggcgat attgtagttt
    13381 ttaaaaccaa tttagttgat ggagaaacag gaaagaaaaa agacttaata aaaagagtta
    13441 tagctactga aggtgacaga ataaaaatat caaattctaa agtgtatgta aatggaaaat
    13501 tattaaatga accatatata cacaataact atacttctgg agatatagat actgttgttc
    13561 caaaaggtaa gctatttgca atgggagata atagagaaaa tagtaatgat agtagattcc
    13621 ctgatgtagg tatggttgat gaggatgaaa ttcttggtaa ggttatggtg agactattac
    13681 ctcttgataa tattgggaaa gtagactaaa aaataagaga ctgaattggt aaagtttatt
    13741 ttacaaatct cagtctcttt ttattttctt aaattaaaaa tattttttta ctaaatatct
    13801 tttgattctg tgataagtct tattatatca cttgcttctt cattaaaagt cgttactata
    13861 ctcaggtagt ctgttgctct actcatagct aaataaacct tatttaaatc tcctacaaag
    13921 tcatttactt gatagtcttg ctcggtatca tttatagttt gattatataa catctctaat
    13981 tcacatacta ttaccgcttt atactctaaa ttctttattg catatatatt tgctattgta
    14041 gttcctacct tcttacttat atttgtcaaa ttatcttctg cacatatata aggaattttt
    14101 gcctcttcca atgctttttt taacatatat tgaaagtaaa tggtttttcc actttttaat
    14161 ttcttcttat tgtaaggata aacgatagca acttcagaat aatcaagacc ttttttactt
    14221 attaaatact caatttccca gattacagaa cttatttggt catcaaggtc acttactttt
    14281 attatatcaa cagacttatt tccacctcta agtgctttag tataaaagaa agtactaaat
    14341 tctgtatttg gtcttaattc gttgatatat gagttagaat tatttgaaaa tttatttgta
    14401 aaatctacaa tctctttagc ttgtctataa tttttactta agataatctt atcatcaaat
    14461 tctataccat tatataattt tttaaatata ttcaatctat tagagatatt caaggagtta
    14521 caagaacaca cattaaatat gaattttgtt ttatataaaa actcccttat aaaatctatt
    14581 tcatactcta aaaagctttc tgcttcatca ataaaaatag ccttgaacat atttttattt
    14641 tttataatgt tctgtgcttg ttttacaaga ttattaaagg tcttttcgta atcatttttt
    14701 aacatgttat aatctacaat taaattgaat tttttagcta gtttaaatat aaaagaacta
    14761 aatgtgtgaa cttctaaatt gttattatct ttgtataata actctatcct ctctcgaagc
    14821 tcattgcaca attgttttgt atgtgtaaat atgactaatt tatgatgtgg atatactttt
    14881 gctaatttta tagccctaga aagcataatt gctgttttgc ctataccaga accaccttca
    14941 ataagtgtat tcccatattt aatagaaata acatctttaa tttgaacctc ttccatcata
    15001 gttgctgtat acttatagtc atcattgtag aatgaaattt ttttaaattt ttcatttaga
    15061 tgaagtttat catttattat atagtattct gaacatatgt ctagtaaaaa taaatttaag
    15121 tttatttcat cattctcatc attaagatat tcctccagcg aacccttatt cattatatct
    15181 tgaagcttat ttttatctat tatattatta ttaacaaatt cttcaaattc atatgtttcc
    15241 tcaacttcaa cgtatggcat aatgtaaacg tagttataac ttatatttct attcttttgt
    15301 cccattttta gtttcaaaag ttcatattcc tcattcataa cttcaagtaa ctcttcttct
    15361 aaaataaaaa atatatcttc tgttgtatcc ataaatttta taaataaaat tcttccatct
    15421 ttaacataca acaaatcggt atttattcct ttaaaaggag taacttttgg cactacttta
    15481 acgtcctcat tgatattttc gtaaaatatt ttctcgaact ccttcaattt tagggcgtct
    15541 aactgagaat ttatatttat gtattggctc acttacatta cctccttctg ttatacttaa
    15601 ttataccatt ttgaactata tttttatata tactttttat aggcaaattt agtaaataaa
    15661 ttcaaaataa atacaaataa atacaaaact gctaaaataa ttttgcaata aa
//
LOCUS       DAEPXF010000086        15471 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain HB27
            SAMN13376389-rid12479423.denovo.091, whole genome shotgun sequence.
ACCESSION   DAEPXF010000086 DAEPXF010000000
VERSION     DAEPXF010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13376389
            Sequence Read Archive: SRR10518432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 15471)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15471)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001069624.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001069624.1
            Genome Coverage       :: 315x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/15/2021 01:54:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,165
            CDSs (total)                      :: 4,076
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 89
            rRNAs                             :: 10, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 10 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 19 of 69
            Pseudo Genes (incomplete)         :: 44 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 10 of 69
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15471
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13376389-rid12479423.denovo.091"
                     /strain="HB27"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="China: Hebei"
                     /collection_date="2017"
     gene            180..1571
                     /locus_tag="KRL67_002310"
     CDS             180..1571
                     /locus_tag="KRL67_002310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HBH1523184.1"
                     /translation="MSSTESVSKKSGHPKSFWLICMTILWERFSYYGLTALLVLFFTA
                     SVAQGGLGLSTQEATSMFGLFTGLIYLTPLVGGWLADKVLGQQKCISIGALLIAIGDF
                     VLFASSTSIAMVWVGLSIIILGNGFFKSSCSNIVGDIYPKTDVSRKDAAYSLFYMAVN
                     VGAFIAPIICGLLSDNVFAVRGASGDIVSYGYKMAFLICGIGMMLGMIIFTLLAPKLL
                     NEVGKYPVTKGNKGEKIEYGPLTKLEKNRIIAMVILFLFVVLFWTAYYQTQSSFMIYV
                     RDNVNRTLFGFEMPVAWLTSLNAILCVALCPVLATLWVKLSHTKRGDFSIPVKMGLGM
                     IIMGVGFIVMVFATLANGPDGMVKASILFIVLTYIFTTVGELFLSPIGLAMFNKLAPA
                     KYATLSMGAWYLTSFFASLLSGKIAGFTATLGFLQIFGGIATVLIIFGLILLGIRNVL
                     TRLMAMDLLTKEK"
     gene            1824..3617
                     /locus_tag="KRL67_002311"
     CDS             1824..3617
                     /locus_tag="KRL67_002311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HBH1523185.1"
                     /translation="MNIKDRLSGLRKLMEEKNIDAYMIPSSDNHQSEYVGDYFKSREF
                     ISGFNGSAGTVIVTKDEAGLWTDGRYFIQAESQLEGSTIKLFKMGQEGCPTTDEYLYK
                     NIPEGGTLGFDGRVISAREGATLAEKLSKKGIKIEYQYDLIDSIWQDRPALSDSKAFL
                     LDVKYCGESFSSKLARLREKMSEKGTSTHVITTLDDIAWLFNIRGGDVKYNPVVLSYA
                     VITLKEVYLFVDESKLNEEILNELAKENVQIKPYNDVYEFVKNIDKTEKVLVDGTKLS
                     YTIYNNIPCEVEKVDEFNPVMFFKAQKNEVELENIRNSHVKDGVAFTKFMYWLKKNVG
                     KMEITEISATQKLEDLRREQEGFFEPSFNTIAAYKEHAAMMHYSATPESNYKLEAEGL
                     FLVDSGGQYYDGTTDITRTTVLGPISDELKLHFTSVARGMINLSKVKFLHGCRGYNLD
                     ILSRSCMWNMGIDYQCGTGHGIGFVLNVHEAPNGFRWRVVPERFDSAVLEEGMVTTNE
                     PGIYIEGSHGIRTENEIVVRKAEKNFYGQFMEFEVVTLAPIDLDGIVPELMNKDEKDY
                     LNWYHKLVYDKISPFLTDEEREWLKVYTRAI"
     gene            complement(3698..5080)
                     /locus_tag="KRL67_002312"
     CDS             complement(3698..5080)
                     /locus_tag="KRL67_002312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HBH1523186.1"
                     /translation="MSVLKTQEKLKQPRDLYFCCSIFTFVGLAYYGMKLVLLLFLAEQ
                     VSKGGLGLTPAESASMLASFLAWTYFSPVIGGWVSDRFLGPKNCIILGMLMVSIGYFL
                     GYIANSEMDINIMIFLSGLGIGFYKGNLHTMVGNLYTNDDPRKDGAFSITYMATNLGT
                     LFGPLICGLVANEWFAMKSGSSISMYGYRYVFLFSSITVLIGLFLFIFGNRKFYKPLN
                     NYPSNGRESIRKSLEYKKMLASRPLTLIEKKRIIVILVLAFFTVFFWIAYNQASMSIA
                     LYTKEHINLTVGNFQIPTSWIDSYNGLLCVILGPISALVWVKLSQTKKGDLSVPKKMS
                     LGFILLAIAFLFMIVAVIQTGTDSNSIHKASVFWVVGFLTFQSIGEICFSPVGYGMVN
                     KLSPEKYISFLMGVWFLGKFAANKLSGYTQAIIDQLGMLQVFIVIPSFLLIFGIILLV
                     MNKKLVELSN"
     gene            5338..5685
                     /locus_tag="KRL67_002313"
     CDS             5338..5685
                     /locus_tag="KRL67_002313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HBH1523187.1"
                     /translation="MIRKKSELQVEVKENLNGGIGKVKLENIVQNEELKGKGRLFKRV
                     ALPVGSSIGVHDHTTDFEVYYILKGKGKVFDNGEFVEVNEGDVVYTADGEKHSIENIG
                     EENLEFVALVLYM"
     gene            5703..6737
                     /locus_tag="KRL67_002314"
     CDS             5703..6737
                     /locus_tag="KRL67_002314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH1523188.1"
                     /translation="MTGKIEEIKDKINRFCNSNLNQEYKDISFKILQNLLDNNKEIIH
                     SSRADIWSSAILNIVLEQNLLYNKKHPLHITKKEFSKQIGVSLNTINNKSSLIKDVCS
                     IDFLNQDTIAISNVEFWVKETNSKSKSVDLDLEKKKILYKRYIKLSQDANSDIESIRY
                     MEEAVNIGKSMITKEDKELGFWKGLSTRAYMIALESLARKLESINNLKESKKVLEYLI
                     EINPSDEQGIRYKLLNVLIRLNDRTSINKLFELYKEEKSATWMYSKALYYFKNKSMFL
                     ANDSIKVARIKNKYVGLYLLDWRNAFGREFVTEEEKAEAVYYYDENIVIWNEVKGSMD
                     WLLKKMLEFS"
     gene            complement(6786..7835)
                     /locus_tag="KRL67_002315"
     CDS             complement(6786..7835)
                     /locus_tag="KRL67_002315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBH1523189.1"
                     /translation="MEINYKKRNFKSFAKRKNIEISVQRYLIDALSYMALGLFSSLLI
                     GTIFNTLGDNFNISLFTEVISPLAKQVTGPAIAVAIAYGLQAPPLVLFSCALVGACGN
                     ELGGPVGAFVATLFAVEVGKLVSKETKVDILVTPAVTILIGVLVAELIGPAVSAFMTS
                     FGNIIMQATTMQPIIMGALVSALVGIALTLPISSAAICMMLSLGGLAGGAATVGCCCQ
                     MVGFAVMSFRENGVGGLVAQGLGTSMLQVPNIIKNWKIWIPPTVASILLGPLSTTIFK
                     MENIPIGSGMGTCGLVGQFGTVTAMQSAGKGGISMWVGILLLNFILPAIVTLLISEFM
                     RKKGLIKPEDLKLDV"
     gene            8110..9645
                     /locus_tag="KRL67_002316"
     CDS             8110..9645
                     /locus_tag="KRL67_002316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH1523190.1"
                     /translation="MIFVKNKKYRKIAIIISIPILLVAQYYLFKFGILRPMVKGVEIE
                     IVDGEYVKDIDKYVVKLNETVTLSTGEYIKVPSYAKDPNIWFNVLDNSGTLKIDGNKM
                     TALKVGYSSVGIMKNSRVLKKATIKVVDPEVESLDMEINGDLKHVGDSATIESTIEVD
                     YDKFKKSYKPVYKSSNENILKIKGNKVSAVGVGKATIYAKCGNKEIERTFRIQARVAE
                     INMDDIKIEVDQNEKLKPDIITSPPGLEPPKIYYELVDSKLPVERAISLSSNGNVVGL
                     REGKDRVRITCGDKSKVITVTVVKETITNDYIQNLVGSHKIVNNKVIIKLEWDYMKDV
                     FDYDIYLKDKLSGESFKKIKSITINENDLGKSNKVYATIEVDMKGNQNVDFDIYVIGK
                     KDSDTTKPSNVINITNGNGNSNNDNNIKDMRVENISANVDRENNVIRVNWSSISHKDC
                     TYSIYVKNNTNGDGEFTLYQSDIQGNEHTISIPEGDLNLDIYVVANYNGNSSQNSDVI
                     NVR"
     gene            complement(9831..10517)
                     /locus_tag="KRL67_002317"
     CDS             complement(9831..10517)
                     /locus_tag="KRL67_002317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate/glutamate racemase family protein"
                     /protein_id="HBH1523191.1"
                     /translation="MKTIGLIGGMSWESTITYYQVINTVIKKRLGGLHSSKCILYSVD
                     FQEIEECQSSGNWEKSAKILADAAIKLQGAGADFIVICTNTMHKVSDKIQESIHIPLL
                     HIADVTATVLKEKEIKKVALLGTKYTMEQDFYKNIIINNGIEVLIPNEEDRIIVNDTI
                     FNELCLGIVSESSKKAFLSIIDKLSKQGAEGVILGCTEIGLLIKQNDTSIPLFDTTVI
                     HAIEAALSSI"
     gene            10642..12024
                     /locus_tag="KRL67_002318"
     CDS             10642..12024
                     /locus_tag="KRL67_002318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HBH1523192.1"
                     /translation="MPINSFENYPMTWKPKLDNRKPPIYKTLAMLLEEDIKRGNLNPG
                     DKLPPQRELADFLDLNLSTITRAFKLCEEKGLICAKVGKGTFISSDVNVSNTLLYQTE
                     SKDIIELGTVHPPYEQNTYIIDFIKNVLKQPEMDRFLQYMSPSGTYMQKKSIAKWIER
                     NNVYTSEENILLSTGGQNAICATLLGLFKAGDRIATDSLSFSGIKSIAKMIGIQLVPI
                     PQENNEISIEYLESYCKNENIKGIYLIPDYHNPTTHTMSDLSRKKIAKIAKQYNIIII
                     EDAINSIFRDGIQTPIFSLASDNTVYIFSTSKFLCAGLRVAFVVAPKRYIENLENALY
                     NMNLMVSPFTAEIVHRLLYSPIIDKIIEEKRDAIIERNEVVDKVLSDYNLIGHKNCSF
                     RWLLLPDELDGKSFEISAKNLGVQVYCAERFSVGNSTVPKAVRICVTAPKDVEELEKG
                     LNIIKSLLKS"
     gene            12426..13694
                     /gene="eam"
                     /locus_tag="KRL67_002319"
     CDS             12426..13694
                     /gene="eam"
                     /locus_tag="KRL67_002319"
                     /EC_number="5.4.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 2,3-aminomutase"
                     /protein_id="HBH1523193.1"
                     /translation="MNEQTRISLERAAELKSKIDDYIQARKTINRGLEKEEEINKRKQ
                     KILSILNGTEEDWNNYKWQLSNRITDVDTLSKIITLTKKEKEYIKEVGTQFRWAISPY
                     YLSLIDPEDICDPIKLLSIPTHIELEDEQEDLDPMGEEYTNPAGCITRRYPDRLIINV
                     TNECAMYCRHCQRRRNIGQQDSHKSKAIIQESIDYIRENEEIRDVLVTGGDALTLKDD
                     YLEWILSQLKEIPHVDYVRLGTRTLVTMPQRITDEFCNMLKKYHPVYINTHFNHPMEI
                     TKESKEACEKLANAGVPLGNQAVLLNGINNDKFVMRCLNQELLKIRVKPYYIFQSKHV
                     KGTKHFNTSVDDGLEIMEYLRGYTSGMAIPTYIVNAPKGGGKTPLLPQYLVSKGTDYV
                     MLRTWEGKVIKMEDEPAVDIKKLIKEQAQD"
     gene            complement(13787..14143)
                     /locus_tag="KRL67_002320"
     CDS             complement(13787..14143)
                     /locus_tag="KRL67_002320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH1523194.1"
                     /translation="MKKALISLLVLSCLLLTAPVNESSYADSQKNVPVSQNTKLTQEQ
                     AKELLVKYNNEVDYIYQGNASDFEALKAKNLNGYVFLPDADGDIGYFVNENTSEIYYF
                     HPSGYLELVNQQQNNK"
     gene            complement(14294..15388)
                     /locus_tag="KRL67_002321"
     CDS             complement(14294..15388)
                     /locus_tag="KRL67_002321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HBH1523195.1"
                     /translation="MQDKKIYLNIVLTVVVSYILIKVIDNYKYFFGVISLLMSLLTPF
                     IIAFVLAYIFNPLVKFLESKLNFKRIYSLLLTYGVLIVILISFILFTVPSIVNSLADL
                     VAQIPTYIDKTEQFLFDLGKSLQSVDPNTLKEYGDKIMSVMPKFSNLLIGSLGGIFST
                     TFSVGKFIVQFLLGFIICFYILLEKEKFLLFTKKVVYISLGKKYGDFIIELCQSLNLN
                     IGKYFTGKILDSFIVGVLSGIGLYFLKSEYALLFGTLMGVMNMVPYFGPVIGMAPVVI
                     INLFSNPTIALTSLIYLIIIQQVEVTFIEPKIVGGQLGLSPFFTILAVTVGGGFFGIP
                     GMILSAPVMGVIKIYFCEFVNRRHDKIQME"
BASE COUNT         5988 a         1927 c         2498 g         5058 t
ORIGIN      
        1 tgaagatact aaaaaaataa tatttttcat aattttaaaa ataataataa aaaaatgaaa
       61 atacaggcga taatctattg agtaaacata gaaaagatga tattataagt atgtgctaaa
      121 atagttaaag caatattaat attaaattta actataaaac aaaagggggc gtttttttta
      181 tgtcaagtac agaaagcgta tctaaaaaaa gtggtcatcc gaagagtttt tggcttattt
      241 gcatgacaat tttatgggaa agatttagtt attatggttt aacagcttta ttggtattat
      301 tttttacagc aagtgtagct caaggtggtt taggtctttc tactcaagaa gcaacatcta
      361 tgtttggttt atttacaggt cttatatatc ttacaccatt agtcggtggc tggctagcag
      421 ataaagtttt aggtcaacaa aaatgtattt ctataggtgc acttttaata gcaataggag
      481 atttcgtact atttgcttca tctacatcta ttgccatggt ttgggtaggt ctatcaataa
      541 ttattttagg aaatggtttt tttaaatcaa gttgctcaaa tatagttggg gatatatatc
      601 ctaaaactga tgtgtcaaga aaagatgcag catacagttt attctatatg gcagtaaatg
      661 taggggcatt tatagctcca ataatatgtg gattgctttc tgataatgta tttgcagtaa
      721 gaggagcaag tggagatata gtatcttatg gatataagat ggcgttttta atttgtggta
      781 taggcatgat gttaggtatg attatattta cacttcttgc accaaaatta ttaaatgaag
      841 tcggaaagta tcctgtgact aaaggcaata aaggtgaaaa aattgagtat ggaccactta
      901 ctaagttgga gaaaaatagg ataatagcaa tggttatttt atttttattt gtggtcttat
      961 tctggacagc atactatcaa acacaaagtt cttttatgat ttatgtaagg gataatgtaa
     1021 atagaactct atttggattt gaaatgccag tagcatggct tacatcacta aatgctatat
     1081 tatgtgtggc tttatgtcca gtattagcta ccctatgggt taaattatct cataccaaaa
     1141 gaggtgattt ctctattcca gttaaaatgg gattaggaat gataataatg ggtgttgggt
     1201 ttatagttat ggtatttgct acattagcta atggtccaga tggaatggta aaggctagta
     1261 tattgtttat agtattgact tatatattta caacagtagg tgaattattc ttatctccta
     1321 taggacttgc aatgttcaat aaattagctc cagctaagta tgccacgtta tctatgggag
     1381 catggtatct aacaagtttt tttgcaagtt tattatcagg aaagattgct ggttttacgg
     1441 ccacacttgg ttttttacag atatttggag gaatagcaac agttttaata atatttggtt
     1501 taatattatt gggaataaga aatgtattaa caagattaat ggctatggat ttactaacaa
     1561 aagaaaaata attcatgacg aattattgta aatagatgaa ttactttaaa taaaggaagt
     1621 tacatttaaa tataaaatta aatgtaattt cctctttgat tatgaaaaaa agctttacac
     1681 aaatagatat tataattaaa attataattg tgataattag agtgtataag atttatataa
     1741 attatataaa aaatatttat gtaataaact ttacatactt atatatttag ttattcaaac
     1801 tattatttca aggaggtctt gttgtgaata taaaagatag attaagtgga ttaagaaaac
     1861 ttatggaaga aaaaaatata gatgcctata tgattccatc ttctgataat caccaaagtg
     1921 aatatgtagg agattacttc aaatcaagag aatttatatc agggtttaat ggttcagcgg
     1981 ggacagttat tgtgactaaa gacgaagctg ggttatggac agatggaaga tattttatac
     2041 aggcagagtc tcaattagaa ggaagtacaa taaaattatt caaaatggga caagaaggtt
     2101 gcccaactac agatgaatat ttatacaaaa atattcctga aggaggaact ttaggatttg
     2161 atggtagagt tatatctgct agagaaggag ctacattagc tgaaaaatta tctaaaaaag
     2221 gtataaagat agaatatcag tatgatttaa tagatagtat atggcaagat agacctgctt
     2281 tatcagatag taaagcattt ttattagatg ttaaatactg tggagaaagt tttagctcaa
     2341 aattagctag attaagagaa aaaatgagtg aaaaaggtac ttctactcat gtaataacta
     2401 ctcttgatga tatagcatgg ttattcaata taagaggtgg agatgttaaa tataatccag
     2461 ttgtactttc ttatgcagta ataactttaa aagaagttta tctatttgtt gatgaaagca
     2521 agttaaatga agaaatacta aatgaactag ctaaggaaaa tgttcaaata aagccatata
     2581 atgatgtgta tgaatttgta aaaaatatag acaaaactga aaaagtttta gtagatggaa
     2641 ctaagcttag ttatacaatt tacaataata taccttgtga agttgagaaa gttgatgagt
     2701 tcaatccagt aatgtttttt aaagctcaaa aaaatgaggt agaattagaa aatataagaa
     2761 attcacatgt aaaagatggt gtagctttta ctaaatttat gtactggtta aagaaaaatg
     2821 taggtaagat ggaaatcact gaaataagtg ctactcaaaa attagaggac ttaagaagag
     2881 agcaagaagg tttctttgag ccaagtttca atacaattgc tgcatataaa gagcatgctg
     2941 caatgatgca ctatagtgca actccagagt caaattataa attagaggca gaaggattat
     3001 ttttagtaga ttcaggtgga caatactatg atggaactac tgatataact agaactacag
     3061 ttttaggacc tataagtgat gaattaaaac tacactttac ttctgtagca agaggaatga
     3121 taaatctttc taaagttaag ttcttgcatg gttgtagagg atataactta gacatactat
     3181 ctagaagttg catgtggaat atgggaattg attatcaatg tggaactgga catggtatag
     3241 gatttgtgtt aaacgttcac gaagctccaa atggatttag atggagagta gtacctgaaa
     3301 gatttgatag tgcagttctt gaagaaggaa tggtaactac taatgaacca ggtatatata
     3361 tagaaggttc tcatggtata agaacagaga atgaaattgt agttagaaaa gctgaaaaga
     3421 atttctatgg tcaatttatg gaatttgaag ttgttacatt ggctcctatt gacttagatg
     3481 gtatagtacc agaattaatg aataaagatg aaaaagatta tttaaactgg tatcataagt
     3541 tagtttatga taaaatatca cctttcttaa ctgatgaaga aagagaatgg ttaaaagttt
     3601 acactagagc tatatagttt gtactagata caataaaaat cgaggtatta taatgcctcg
     3661 attttttatt gtatatttta ttaactttaa gaaattttca atttgataat tctactaatt
     3721 ttttattcat tacaagtagt ataattccaa atatcaataa aaatgatggt atcactatga
     3781 agacttgtaa cataccaagt tggtctataa tagcttgcgt atagccagat aatttatttg
     3841 ctgcaaattt gcctaaaaac caaacaccca ttaggaatga aatatacttt tcaggcgaaa
     3901 gtttatttac cataccatac ccgactgggg aaaagcaaat ttctcctata ctttggaatg
     3961 ttaaaaatcc aactacccaa aatacacttg ctttgtgaat tgaattagag tctgttcctg
     4021 tttgtataac tgctactatc ataaataaaa aagctatagc taatagtata aagcctaagc
     4081 tcattttttt tggcacactt aaatcacctt ttttagtttg agataatttt acccatacta
     4141 aagcagagat aggtccaaga ataacacaaa ggagaccatt atatgaatct atccatgaag
     4201 taggaatttg aaaattgcca acagtaagat ttatgtgttc ctttgtatag agtgcaatag
     4261 acatagaggc ttggttatat gctatccaaa agaatactgt gaaaaaagct aacaccaata
     4321 ttactattat tctttttttc tcaatcaaag ttaaaggtct tgaagcgagc attttcttat
     4381 attctagtga ttttcttata gactctctcc cattactagg gtaattattc aaaggtttat
     4441 aaaactttct gtttccaaat ataaagagga ataaacctat tagaaccgta attgatgaaa
     4501 ataagaatac atatctatat ccatacatac ttatagagga tccgcttttc atagcaaacc
     4561 attcattagc aaccaaaccg catattaaag ggccaaataa agtacctaga ttagtagcca
     4621 tgtacgtaat agagaaagct ccatcttttc ttgggtcatc attggtatat aagtttccaa
     4681 ccatagtatg taagtttcct ttataaaaac ctattcctaa tccagataaa aatatcatga
     4741 tgtttatatc catctcacta tttgctatat atcctagaaa ataaccaatt gaaaccatta
     4801 gcattccaag tataatacag tttttgggac ctaaaaatct atcactgacc catccaccga
     4861 taactggaga gaaataagtc catgctaaaa aagatgcaag cattgatgcg gattctgctg
     4921 gtgttaaccc taaaccacct ttggagactt gttcagctaa aaacaataat aataccaatt
     4981 tcataccata gtatgcaagt cctacaaagg taaatattga acagcagaaa tataaatctc
     5041 taggttgttt aagtttttct tgcgttttta aaactgacat atgttgagaa cctccagata
     5101 aatagtttaa caaaatttta ccatactata tgagtatatg tttttttcat atagacgtca
     5161 acttatgaga taatttaatt aagtcgaaat atatgtaaaa atagtatcat ttttgtgtat
     5221 tcagtaatat ttttttatga taatatatgt tattattata ttaaaataca aacatattta
     5281 tggaaaggat atgccaattt agtagttaga ttgattgttt ggcaggtggg atgagctatg
     5341 ataagaaaga aatctgaatt acaagtagaa gtaaaagaaa atttaaatgg tggtatagga
     5401 aaggttaagc ttgaaaacat agtacaaaat gaagagctta aaggaaaagg aagactattt
     5461 aaaagagtag ctttaccagt aggaagttca attggtgtgc atgaccatac aacagatttt
     5521 gaagtatatt atatactaaa gggaaaaggt aaagtatttg ataatgggga atttgtagaa
     5581 gtaaatgagg gtgatgtagt gtatacagct gatggcgaaa aacattctat agaaaatata
     5641 ggagaagaaa atcttgagtt tgtagcacta gttttatata tgtaaaatat ggaggaggag
     5701 tcatgacagg caagatagaa gaaattaagg acaagataaa tagattttgc aactccaatt
     5761 taaatcaaga atacaaagat ataagcttta aaattttgca aaacctttta gataataata
     5821 aagagataat tcattcttct agagctgata tatggtcttc agctatacta aatatagttt
     5881 tagaacaaaa tcttttatat aataagaagc atccactaca tataacaaaa aaagagtttt
     5941 caaaacaaat aggtgtttct ttaaatacta taaataataa atcgtcttta ataaaggatg
     6001 tttgtagtat tgacttttta aatcaagata ctatagctat ttctaatgta gaattttggg
     6061 taaaagagac caattccaaa tcaaagtctg tagatttaga tttagaaaag aagaaaatac
     6121 tatataaaag atatattaag ctatcacaag atgcgaatag tgatattgaa tcaattagat
     6181 atatggagga agctgtaaat ataggtaaaa gtatgattac taaagaagat aaagaacttg
     6241 gattttggaa aggtttatct accagagcct atatgatagc acttgaatcc cttgctagaa
     6301 agttagaaag tattaataat ctaaaggagt ccaaaaaagt attagaatat ttaatagaaa
     6361 ttaatcctag tgatgagcag ggaataagat acaaattatt aaatgtatta attagattaa
     6421 atgatagaac ttcaataaat aaattatttg aactttataa agaagaaaaa tctgctacat
     6481 ggatgtacag taaagctctt tattacttta aaaataaaag tatgtttttg gcaaatgatt
     6541 ctattaaggt tgctagaatt aaaaataagt atgttggatt atatttgtta gattggcgaa
     6601 atgcttttgg tagagaattt gttacagagg aagaaaaggc agaagctgtc tattactatg
     6661 atgaaaacat agtgatttgg aatgaagtta aaggttctat ggattggcta ttaaaaaaaa
     6721 tgttggaatt ttcataaaaa aaactactcc ttttacttat ataaaagtaa ggagtagttt
     6781 ttaatttaca catctagttt taaatcttca ggttttatta gacctttttt tctcataaat
     6841 tctgaaatca acaaagttac aatagcagga agtataaaat taagtaaaag tataccaacc
     6901 cacatagata tacctccctt tccagcactt tgcatagcag ttactgttcc aaattgtcca
     6961 accaatccac aagtacccat accagaacca ataggaatat tttccatttt aaatattgtt
     7021 gtagataatg gtccaagtaa tattgaagct acagtaggtg gtatccaaat tttccaattt
     7081 tttattatat taggtacttg caacatagat gtcccaagac cttgtgccac aaggccacca
     7141 actccatttt ctctaaagct cataacagca aagcctacca tttggcaaca acaacctaca
     7201 gttgctgccc caccagcaag accacctaaa cttaacatca tacaaatagc tgcactactt
     7261 ataggtaatg taagagctat tccaacaagt gcagaaacta aagctcccat tattataggt
     7321 tgcatagttg tagcttgcat tattatattt ccaaatgaag tcataaaagc agaaacagct
     7381 ggaccaataa gctcagctac aagtactcca attagtattg tcacagcagg tgtaaccaat
     7441 atatctactt ttgtttcttt tgaaactaat tttccaacct ctacagcaaa aagtgtagcg
     7501 acaaatgctc caacaggtcc tcctaattca ttaccacaag ctccaacaag tgcacaagaa
     7561 aatagtacaa gtggaggtgc ttgaagtcca tacgcaatag ccactgctat agctggccca
     7621 gtaacctgtt tagcaagagg gcttataact tcagtaaaaa gagaaatatt gaaattatct
     7681 cctaatgtat tgaaaattgt tccaattaaa agggaagaaa aaagaccgag tgccatataa
     7741 cttagtgcgt caataagata cctttgaaca gaaatttcaa tgttttttct tttagcaaat
     7801 gatttgaaat ttcttttttt ataattgatt tccaatttaa aatcctcctc atataatata
     7861 agtataattt ctttttaatt aattatataa cttttcagct gacaagacaa atgttatttc
     7921 actaaaatta gactattgta atagtaagaa attaacatta attttagttt taaattttaa
     7981 atttatggtt tataattagt atagggatat tctgaaatat aataattttc cagtttatcc
     8041 caatatttaa cttaggtaga ttgcttacaa aacaaatgat tatatattag gctaaaagga
     8101 agtggataga tgatatttgt aaaaaataag aaatacagaa aaattgccat aattatatct
     8161 attcctatat tgcttgttgc gcagtactac ctttttaaat ttggaatact tagaccaatg
     8221 gtaaagggag tagaaattga aattgtagat ggggaatatg ttaaagatat agataaatat
     8281 gtagtaaaat taaatgaaac agttactttg tctacaggtg aatatattaa ggttccttcg
     8341 tatgctaaag atccaaatat ttggtttaat gtattggata attcaggaac tttaaaaata
     8401 gatggaaata aaatgacagc attaaaggtt gggtattctt ctgtgggtat catgaaaaat
     8461 tcaagagtat taaaaaaggc tactataaaa gtagtagacc cagaggtaga gtcactagat
     8521 atggagatta atggggattt aaaacatgtt ggtgatagtg ctactataga aagcacaata
     8581 gaagttgatt atgataagtt taaaaaatca tataagccag tttataagag tagcaatgaa
     8641 aatattttaa aaatcaaagg aaataaagta agtgcagttg gagttggtaa agctacaata
     8701 tatgctaagt gtggaaataa agaaattgag agaacattta ggattcaagc tagagttgct
     8761 gaaataaata tggatgatat aaaaatagaa gttgaccaaa atgaaaaatt gaaaccagat
     8821 ataataactt ctccacctgg cttagaacca ccaaaaatat attatgagtt agtagattca
     8881 aaattacctg tagaaagagc aataagtttg agtagtaatg gaaatgttgt aggccttaga
     8941 gaaggtaaag atagggtgag aataacttgt ggagataaat ctaaagtaat aacagtaaca
     9001 gtagtcaaag aaactataac gaatgactat atacaaaact tggttggttc acataaaata
     9061 gtaaacaata aagttattat taaacttgaa tgggattata tgaaagatgt ctttgattat
     9121 gatatatatc ttaaagataa gttgtcagga gaatcattta agaaaattaa atctataact
     9181 ataaatgaaa atgatttagg taagtctaac aaagtatatg caactataga agtagatatg
     9241 aagggtaatc aaaatgtaga ttttgatatt tatgttatag ggaaaaagga ttcagataca
     9301 accaagccaa gtaatgtaat aaatattact aatggaaatg gaaattcaaa taatgataat
     9361 aatattaaag acatgagggt agaaaatata tcggctaatg ttgatagaga gaataatgtc
     9421 attagagtta actggagtag tatatctcat aaggactgta cttacagtat atatgtaaaa
     9481 aataatacta atggagatgg agaatttact ctatatcaaa gtgatataca gggaaatgag
     9541 catactataa gtattcctga aggagattta aatctagata tttatgttgt tgcaaattat
     9601 aatggaaata gctctcaaaa tagtgatgtt ataaatgtaa gatagatttg tatacaaatt
     9661 gaaagtgttg agtttattta aatattaaat taagaaaaag gatagtatag ggtaattata
     9721 ctatcctttt tttgttacaa tatataggtt taaaatctcc agtaatataa attataaaaa
     9781 gtcttattta cacaaatcta tagatattct ttttgttaat taatattgtc ttaaattgaa
     9841 cttagtgcgg cttctattgc atgtattact gttgtatcaa atagtggaat agaagtgtca
     9901 ttttgtttaa ttaaaagacc tatttctgta catcctaata taacaccctc tgcaccttgt
     9961 ttgctaagct tatctataat agataagaaa gctttttttg aagattcaga tacaattcct
    10021 aagcaaagtt cgttaaatat tgtatcattt actattatac ggtcttcttc atttggaatt
    10081 aagacttcaa ttccattatt aataattatg tttttataaa aatcttgttc cattgtatat
    10141 ttagtaccta ataaggccac tttttttatt tctttttcct ttaataccgt agcagttaca
    10201 tctgctatgt gaagtaatgg aatatgtata ctttcttgta ttttatctga taccttatgc
    10261 atagtattag tacagataac aataaaatct gcccctgccc cttgtaattt aattgcagcg
    10321 tcagctaaaa tcttggcgct tttttcccaa tttccactag attggcattc ttcaatttct
    10381 tgaaaatcta cactgtaaag tatacactta gaagagtgta gtccacctaa tctttttttt
    10441 attactgtat taataacttg ataatatgta atggtacttt cccaactcat accaccaatt
    10501 aagcctatag tcttcatatt atacctctct tatttattta ttttgaaata gctatttaca
    10561 aatacaagta taacatctaa aatagaagca tacaataata aaattgtatg gagtacaatt
    10621 ataattaaga ggagtaatta tatgccaata aattcttttg aaaactatcc tatgacatgg
    10681 aaaccaaagt tagataaccg aaaaccacca atatataaaa ctttagcaat gttattagaa
    10741 gaagatatta aaagaggcaa tttaaatcct ggagataaac tgcctccaca acgggaatta
    10801 gcagattttc tagatttaaa cttaagtaca ataacacgag catttaaact ttgcgaagaa
    10861 aagggactaa tatgtgcaaa agtgggaaag ggaacattca tttcttctga tgtaaatgta
    10921 agtaatacat tattatatca aacggaatca aaagacataa ttgagttagg gacagttcat
    10981 cctccgtatg agcaaaatac atatattatt gactttatta aaaatgtact aaaacaacca
    11041 gaaatggata ggtttttaca atacatgtct ccatctggca catatatgca aaaaaagtca
    11101 attgcaaaat ggatagaaag aaataatgta tacaccagtg aagaaaatat attattatct
    11161 acaggaggtc aaaatgctat atgtgcaact ttactaggat tatttaaagc tggtgataga
    11221 attgctacag attctttaag tttttctgga attaagtcaa tagctaaaat gataggtatt
    11281 caattagttc caattccaca agaaaataat gaaatatcaa ttgaatatct tgaaagttat
    11341 tgtaaaaatg aaaatataaa aggaatatat cttataccag attatcataa tccaacgaca
    11401 catactatgt ctgatttatc tcgtaaaaag atagccaaaa tagcaaagca atataatata
    11461 ataatcattg aagatgctat aaatagcatt tttagagacg gtatacaaac tcccatattt
    11521 tctttagcta gtgataatac tgtatatatt tttagtacat ctaaattttt atgtgctggt
    11581 cttagagtag catttgtagt tgctccaaaa cgatatatag aaaatttaga aaatgcacta
    11641 tataatatga atctaatggt ttctccattt acagcagaaa tagtacaccg tttattatat
    11701 tctcctatta tagataaaat tatagaagag aaaagagatg caattataga aagaaatgaa
    11761 gttgttgata aagttttgtc agattataat ttgattgggc ataagaattg cagttttcgt
    11821 tggttacttc ttcctgatga attggatgga aaatcatttg aaatttctgc aaaaaatcta
    11881 ggagttcaag tatattgtgc tgaacgattt tcagtgggaa actctacagt tccaaaagcc
    11941 gttcgtattt gtgtaacagc tccaaaagat gtagaagaat tggaaaaagg gctcaatata
    12001 ataaagtcac ttcttaagag ttaaatttat tttactatta tttaattaaa taaataaaaa
    12061 ggcggagaaa tttattataa taaacctccg cttttattat ttaatgaaat actttacttt
    12121 ttattaaaaa atttaatttt aataatcttg aaaatataat cgttttatgg taaaataatt
    12181 cttaaaaata gaaaaaatta atcatttgtt aaaaaaaatg cataatttat tgtaatttat
    12241 tgacgaaggt tgaaaaatat tcataaatgt gcgaaataac agaaaaaatg caaaaattaa
    12301 ttcaatttca tttgttataa aatctatatg gcaaaattaa atctgcctat cagaatgata
    12361 taaacacatc agaaaggagg acgctatcaa ataatacaag tagttaactt tgatagcaaa
    12421 aaattatgaa tgaacaaact agaatatcct tagagagagc tgctgaatta aaatcaaaaa
    12481 ttgatgatta tattcaggct agaaaaacga ttaacagagg tcttgaaaaa gaagaagaga
    12541 taaataaacg aaaacagaaa atattaagta tcttaaatgg aactgaagag gattggaata
    12601 actacaaatg gcaattatca aatagaataa cagatgtaga tactttatca aagattataa
    12661 ctctaactaa aaaagaaaaa gaatatataa aagaggttgg tactcaattt agatgggcaa
    12721 tatctccata ttatttgagt cttatagacc cagaagatat atgtgaccca ataaaattac
    12781 tatctatacc aacacatata gagttggaag atgaacaaga agatttggac ccaatgggag
    12841 aagaatatac aaacccagca ggatgtataa ctagaagata cccagataga ttaataataa
    12901 atgtaacaaa tgagtgtgct atgtattgta gacactgtca gagaagaaga aatattggac
    12961 aacaagattc tcataagtca aaagctatta tccaagaatc tatagactat atcagagaaa
    13021 atgaagaaat aagagatgta ctagtaactg gaggagatgc tcttacatta aaagatgatt
    13081 atttagagtg gattcttagt caacttaaag agataccaca tgttgattat gttagattag
    13141 gtactaggac tcttgttaca atgccacaaa gaattacaga tgaattttgc aacatgctaa
    13201 aaaaatatca tcctgtatat ataaatactc attttaatca tccaatggaa ataactaagg
    13261 aatctaaaga agcttgtgaa aagttagcaa atgcaggagt tccattaggg aatcaggcag
    13321 tattattaaa tggaataaat aatgataaat ttgtaatgag atgtttaaat caagaattac
    13381 tgaaaataag agtaaaacct tattatatat tccaaagtaa acatgtaaag ggaacaaaac
    13441 atttcaatac atcagtagat gatggtcttg aaatcatgga gtatttaaga ggatatacat
    13501 caggaatggc tataccaaca tatatagtaa atgctccaaa aggaggagga aagactcctt
    13561 tgcttccgca ataccttgta tcaaaaggaa cagattatgt tatgcttaga acatgggaag
    13621 gaaaagttat aaaaatggaa gatgaacctg ctgtagatat aaagaaactt ataaaagagc
    13681 aagcacagga ctaaaaaagt atttaaagtc tataaaaaga aacaacttta aaataaaaag
    13741 aaaatggatg ccgaatttat atcaatttgc atccattttt tattagttat ttattatttt
    13801 gttgttgatt aactaattca agatatccac ttgggtggaa gtaatatatc tcagaagtat
    13861 tttcatttac aaagtaacca atatcaccat ctgcatctgg taaaaataca taaccattaa
    13921 gatttttagc ttttaatgct tcaaaatcac tagcatttcc ttgatatatg taatcaactt
    13981 cattgttgta ttttactaaa agttctttag cttgctcttg agtcaattta gtattttggc
    14041 ttacaggaac attcttttga gaatctgcat aagaagattc atttactggt gcagttaata
    14101 acaaacaaga taaaactaat aaagatataa gtgctttttt catactaaaa ccctctttca
    14161 tgtttaatta tataagattc attacttaat aatattgtat gtttcctata attaaatatt
    14221 accattaaag agaataatat tacagttaca cagctgtaac ttttgattac aaaatcgtaa
    14281 ttaaattgta tttttattcc atttgtatct tatcatgtct acgatttaca aattcacaga
    14341 aatatatctt tataacaccc atcacaggag cagatagaat cataccaggt ataccaaaga
    14401 atcctccacc aacagtaact gctaggattg taaagaatgg gctaagacct agttgtccac
    14461 ctacgatttt tggttctata aatgtaactt caacttgttg aattattatt aagtaaatta
    14521 aagaagttaa agcaattgtt ggattggaga ataaattaat tattacaact ggtgccatac
    14581 ctattactgg accaaaatat ggaaccatat tcatgacacc cataagtgta ccaaataata
    14641 gtgcatattc agattttaaa aagtaaagac caattccaga gagtactcca actataaaag
    14701 aatctaaaat cttacctgta aagtatttac caatatttaa atttaagctt tgacatagtt
    14761 ctattataaa gtctccatat tttttaccta aggaaatata aactactttc tttgtaaaca
    14821 gtaagaattt ttctttttct aataatatat agaaacaaat tataaaacct aaaagaaatt
    14881 gtactataaa ttttccaact gaaaatgtag tgctaaatat tccaccaagt gaaccaataa
    14941 gcaagttact aaatttaggc attacactca taattttgtc accatactct tttaatgtat
    15001 tagggtcaac tgattgtaaa ctcttaccta agtcgaataa gaactgctct gttttatcta
    15061 tgtatgtagg aatttgagca actaaatcag ctaaactatt tactatggat ggaactgtaa
    15121 ataatataaa ggaaattagt ataactatga gtacaccata agtaagaagt aacgaatata
    15181 ttctcttaaa atttaattta ctttctaaga actttactag tgggttgaat atatatgcta
    15241 atacaaatgc tattataaat ggtgttagta gtgacattaa taaacttatc actccaaaaa
    15301 aatatttgta attatcaata actttaatca agatatatga aacaactaca gtgagtacaa
    15361 tatttagata aattttttta tcttgcaaaa taacacaacc tttcttatat ttattaaatt
    15421 tagatatact taaatctaag ttaaagttaa tatttatagc ttattataca a
//
LOCUS       DAEPXF010000087        15413 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain HB27
            SAMN13376389-rid12479423.denovo.092, whole genome shotgun sequence.
ACCESSION   DAEPXF010000087 DAEPXF010000000
VERSION     DAEPXF010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13376389
            Sequence Read Archive: SRR10518432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 15413)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15413)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001069624.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001069624.1
            Genome Coverage       :: 315x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/15/2021 01:54:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,165
            CDSs (total)                      :: 4,076
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 89
            rRNAs                             :: 10, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 10 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 19 of 69
            Pseudo Genes (incomplete)         :: 44 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 10 of 69
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15413
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13376389-rid12479423.denovo.092"
                     /strain="HB27"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="China: Hebei"
                     /collection_date="2017"
     gene            517..1428
                     /locus_tag="KRL67_002322"
     CDS             517..1428
                     /locus_tag="KRL67_002322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YafY family transcriptional regulator"
                     /protein_id="HBH1523196.1"
                     /translation="MKINRLTEIIVILLNKKLVTAKELADRFEVSTRTIYRDIETLSM
                     SGVPVYMTKGKGGGISLIEEYSIDKTILSKKDKESLIVALKTLQATKYPEINSVVNKI
                     GSIFGEQNFSNWIEIDFTEWGSNFNEDDKFTKIKEAILRCNTINFNYVNSLSSQTNRT
                     VEPLKLMYKSKTWYLYGFCKLKDDFRIFRISRIRNLSIKDEVFSRKIIEEACLNDSKV
                     IKENTITLKLRFKEKMLFRVFDDFNKDLITKNEDDTYDVITEFPIGEWIYGYILSFGD
                     NVEVLEPKDVRDNVITRLRELSKIYSL"
     gene            1603..2100
                     /locus_tag="KRL67_002323"
     CDS             1603..2100
                     /locus_tag="KRL67_002323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="effector binding domain-containing protein"
                     /protein_id="HBH1523197.1"
                     /translation="MNYEIVEISEKIVIGISKETTNKDGQAVNDIGELWKKFMGKGIY
                     NAIKGKKSDKTIGLYTDYQGDFTSPYNFVACCEVDSNSDKEKNLEELNTNNTVGESII
                     SKVIPAGKYAKFVIVGGQKEVGDFWFEFWQMDFDRTYISDFEEYQGNTFDTNKQEIHI
                     YIGIK"
     gene            complement(2380..2739)
                     /locus_tag="KRL67_002324"
     CDS             complement(2380..2739)
                     /locus_tag="KRL67_002324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HBH1523198.1"
                     /translation="MTHIYFNGQCKQAIELYKQAFNAIVKTLIEDSSNLLVVHAEIFI
                     HNELLILNDFGNKDGISISGGYQLCVQFDNETDLLKAYNTMKNDSTTIEPMQSTDYSS
                     CVVRFIDMFNVRWAFWV"
     gene            3535..3897
                     /locus_tag="KRL67_002325"
     CDS             3535..3897
                     /locus_tag="KRL67_002325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HBH1523199.1"
                     /translation="MENKILIVDDAIFMRMIIKEALSKSGYNNLIEANDGEEACNIFK
                     YEKPDLMLLDITMPKKDGLEVLREIKKLDSSAKVVMCSAIGQENMIVEAIKLGALDFI
                     VKPFKTETLVKVVNNVLK"
     gene            3962..4579
                     /locus_tag="KRL67_002326"
     CDS             3962..4579
                     /locus_tag="KRL67_002326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheC"
                     /protein_id="HBH1523200.1"
                     /translation="MINYLELDDVHIDALREIGNIGSGNAITALASMINSNVEVLIPM
                     VKILEYNEATNLLGGPENKVVCILLDMKGDINGMFMFLLDESITQLMLSSLFNKEEST
                     LDEIEAIEISAIKEIGNIMASSYVNAIASMLNMTISVSIPDICIDMVGAVLNVPMIRF
                     SDVGDKVLFIENKFKMSDNYFTSHILMIPEMSSLREILVRLGLEV"
     gene            4576..5061
                     /locus_tag="KRL67_002327"
     CDS             4576..5061
                     /locus_tag="KRL67_002327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheD"
                     /protein_id="HBH1523201.1"
                     /translation="MNREIVVNIADMKIAYRPNVLVTYALGSCVGVCLIDKVAGIGGM
                     LHVMLPYSKDAINIENKCKFADTGINELIKSMENIGANRIYIKAKIAGGAQMFLGSSN
                     SVIASIGHRNVEAVKKTLLGLNIPILAEDTGMNYGRTIRFYTETGELLVDSINKGKKK
                     L"
     gene            5104..5622
                     /locus_tag="KRL67_002328"
     CDS             5104..5622
                     /locus_tag="KRL67_002328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-binding chemotaxis protein CheW"
                     /protein_id="HBH1523202.1"
                     /translation="MRFPDDPYLDYGLIDEGIKYLKFKVDEQDFGVELEKVIEITGNQ
                     EIIFIPELPTYSKGIINLRGKIIPIIDMRLRLKKDELMTTNCMYIVVTEIKDLVVGFV
                     VDRVDDIVSLEKSKISPPPRVTIEYSDYFVSGVGELEGKIITLLDLEKLLTDKDEILI
                     DELINGFDCTIM"
     gene            5758..6717
                     /locus_tag="KRL67_002329"
     CDS             5758..6717
                     /locus_tag="KRL67_002329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HBH1523203.1"
                     /translation="MKNKQQGTILVVDDSVLMCDLIDKTLTKDNFKVKKAFNAIEAKE
                     FIEEFIPDIILLDIILPDNSGFEFCKEVKSNARTADIPIIFITSKNTDTDIVKGFGVG
                     AIDYMVKPFSMAELKARVIAHLEVKKSQDKLKKINNELESSLEKLNRLVVRDYLTGLY
                     NRRHIINQLMEQRRINKYSETSISLILGDIDDFKVINDTYGHEAGDFVLTVISSIIKK
                     NCRQIDTVSRWGGEEFLIMLTNTPIEGAKILSERIRKEIKEFDFNYKENKIKCTITLG
                     VVEVNSNISLEENISLADKAMYEGKNLGKDCSVVSTMKGLKKI"
     gene            6743..8446
                     /locus_tag="KRL67_002330"
     CDS             6743..8446
                     /locus_tag="KRL67_002330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing protein"
                     /protein_id="HBH1523204.1"
                     /translation="MKREVKLSKLAIVLLIIIVLAVLAGIFSSISSYKAFEKVEKSIN
                     DMNNLRTQSQYIKDTTREISDVLRRYVFTGEKKNKDIYEKELYGKTRESALNKLEKIG
                     LTKSESNKILEAKEISDSLVPTEQSAIKAVESNDKSKAQSIIFGSDYINSQEKMDSLI
                     NEFQEDIVTRSNSKIVEVKKELQSLILRTIFILSLLIILTIIEYTVIKLKIVIPINKV
                     EKHFSRIASGDLRTSIDVEESKSEIGLLVSSVKKMQSMLLNYIDLIDSTLTSIAKGDL
                     RVEIDQEFIGDFKSIKISLESIINSFNGDFSEINLSAEQVASASEQVAAGAQALSQGA
                     TEQASSVEELASTINEISDGIKKCADSASLACEVSDQSASQVGFGSRCMDDMMTSMGE
                     ISEKSSEISKIIKTIDEIAFQTNILALNAAVEAARAGQYGRGFAVVADEVRSLAGKSA
                     KAAQNTAILIEEAIKTVENGVRIAKETANALDLVVNGVEKTTNYINGISSAVHSQEVA
                     ITQILSGIEQISIVVQMNSATAEESAAASEEMSAQAQILKELVEKFHLKESEVRESSW
                     V"
     gene            8617..10719
                     /locus_tag="KRL67_002331"
     CDS             8617..10719
                     /locus_tag="KRL67_002331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheA"
                     /protein_id="HBH1523205.1"
                     /translation="MRQMLDMFIFETSLLLEQLDEIMIQIEREKVFTHNNINETFRIM
                     HTIKGSASMMGLDNISALAHSVEDLFYAIREGKIEVLDNDLLFDLVFQSLDFIRRDID
                     ELQSRGCISSDYSNFIEGIEEYVSTLELSSSMKSKDNANLENTDEKEVINSNFAYKED
                     SDVVKIIFEQDCMMENVRAFMLVFELRDYCEFIDFYPKDVETNAESTEYIKSYGFYIQ
                     FVAKENKEIVYKTIEDNLYVKEYINISKKEIEMKSTSNNDAVENKLKDNNKVSKGYQN
                     NYTEDELQEDVLNEKIDIEKDSLSEENEHLPISKQSIMSVNLEKLDLLQNIVGEIVIM
                     ESMVVNSVNSTGVDIDNFNKATRQLHKLTDELQDIVMSMRMIQIAGVFQKMHRIVRDM
                     NKKLDKNVELITIGAETEVDKSVIDNLAEPFMHLIRNAMDHAIEPIEERIAKGKEEVG
                     KIVLSAENLGGEAVISISDDGRGIMRDKILAKAKEQGLLKHQSNEYTDDEVNSLIMTP
                     GFSTKESITEFSGRGVGMDIVRKSIEKVGGSIEVKSKQDEGTTFTIKIPLTLSIVEGM
                     KFKVGDSLFILPISSIRRTFKLTDLNQIITEGNKTELITIYGACIPIIRLHKIYNIQT
                     KVTNLMHGNMIIIENEHKAVCIFVDEIIGNQQVVVKPFPVYFNRFNIKNNGLSGCTIL
                     GDGSISLIIDADSIIEIY"
     gene            10733..11173
                     /locus_tag="KRL67_002332"
     CDS             10733..11173
                     /locus_tag="KRL67_002332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HBH1523206.1"
                     /translation="MAKDEIIKQVLTFYVNDVIYGIELENVIETIRFQSITYVPCLPT
                     YISGVINLRGRIIPVINMHIKYNLPKADYNERTCIIITKVDEYQVGIIVDKVVDVITI
                     ENLNLLETTNSNNTNINKDIKEIAKVEDNSILILDIRKFLINSM"
     gene            11209..12012
                     /locus_tag="KRL67_002333"
     CDS             11209..12012
                     /locus_tag="KRL67_002333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate O-methyltransferase CheR"
                     /protein_id="HBH1523207.1"
                     /translation="MIKLTDEEFIVLVNHVKKEYGIDLSKKRALIEGRLYNTMIEKKL
                     SSFSQYMNLLFKDKTGNEAINLINRLSTNHTFFMREPQHFEFIQNSILPFWEENNKIR
                     NINIWSAGCSSGEEAYSIAMTLDDYFGYNKELWNIKIIATDISTNSLEKAKRGIYIES
                     NVNNVPELWKKKYFIDNKDGTFKICDKIRKSVIFKPFNLMNDFSYQHFDLIFCRNVMI
                     YFDLKTREELINKFYNATKKDSFLFVGHAEVINRSSTKYKYVKPAIYKR"
     gene            12052..12648
                     /locus_tag="KRL67_002334"
     CDS             12052..12648
                     /locus_tag="KRL67_002334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheB"
                     /protein_id="HBH1523208.1"
                     /translation="MERFNNMVIAIGASVGGTEAILEIIKDLPKSTPGIVVVQHMPAI
                     FTYMYAQRLDKQCIMNVREAKNNDRVEQGNVLIAPGGYQMKLCTDKQGYYVTCEKGER
                     ISGHCPSVDVLFDSVAEVAGKNSIGIILTGMGYDGANGLLKMKENGSFTIGQDENSCI
                     VYGMPMIAFDRGSVMLQLPLGEISNCLIRHINILKQKD"
     gene            12754..13617
                     /locus_tag="KRL67_002335"
     CDS             12754..13617
                     /locus_tag="KRL67_002335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3298 and DUF4163 domain-containing protein"
                     /protein_id="HBH1523209.1"
                     /translation="MKFFKVDTKARVLRYVFVLTLTIMAVLAIKHDYKYNNLRKDASI
                     NLNSNKEAVTVMSNQSLPKGNTNSKQTLSVSTIGSKTIVKNDEYLESTINMPVITNSN
                     KIVERSTNDKIKNDIFEFYNKSYKEAKQYLKDNPDEKNKFVANVDFELKKNTDSALSI
                     KVRYYTYSGGAHGFYQDIAYNVDMRTGKFLELMDLFKDNTKYTEVIDEEIKRQIAELE
                     KKDEENIGIYNFKGIKENQNFYLQDENLVVYFDLYDITPYAAGIPEFSINKALISSML
                     KPEYVDLFDLK"
     gene            13684..14673
                     /locus_tag="KRL67_002336"
     CDS             13684..14673
                     /locus_tag="KRL67_002336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HBH1523210.1"
                     /translation="MLLLKKDDIEKVFTMRDAIEADKEAFRIYCEGKSVNPLRTNISV
                     PSQDASMLFMPGYVEELGCGGIKIVSVFPKNAQKGKPVIPATVLLLDGETGEVSAVLD
                     GTYVTQIRTGAASGAAIDVLAKKDSKIGALIGLGGQGEPQLEAMIEARNLDEVRVFSR
                     NKESREKFAEEMNLKLSKYNTKIVAVESSDKAIDNADVIVLATPSKQPVFNGNLVKKG
                     ALISAVGSYMPDMQELCPNCLTRASKIFFESTDAVLSESGDILIPLKEGKISKDDFSG
                     DLGNVINGTIPGREDDDEIIVFKTVGIGVQDVVTAKRIYDKAKENGIGMDWKW"
     gene            14764..15375
                     /locus_tag="KRL67_002337"
     CDS             14764..15375
                     /locus_tag="KRL67_002337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH1523211.1"
                     /translation="MKMVKKLILSSIMALVLVLTIGCSNAEEKVKSNFEANLAALEMY
                     KYDEKGISDALTEAEYKQIQTYNSKDKVSMIAQLTPLYGKENAEKIYETIVKLIQGVE
                     KTVTVESSSKDKITLKVVSKALDTSKVMSEANTLVKSYVTDEKNYDKKEKEVQDDMTK
                     MIIDLYNKKPTKEVTTMITYTKKDGKWQPPSNIQKLCEGIYTI"
BASE COUNT         6273 a         1476 c         2819 g         4845 t
ORIGIN      
        1 ttgaaaaata ataatataga attgagaaat agtaaattga aattttaaaa taaatgagta
       61 gaaggagctt agaaagtatg gaaatggata ttacaataaa aaataaataa tatggaaaga
      121 ctttaaatta attttaagta ttaagtacaa atactaattt ttgatataga aattgaataa
      181 agtaaatgat aataagcaaa tgaattaaga tgataataag tgagataata ataagttagt
      241 tggtatagca ataagtaaac taagtaagat gataacaaat gagaggataa taagtgaatt
      301 gatatagcga taagtaaatt aagtaagacg ataataagtg aattgatata gtaataaata
      361 aattaagatg ataatatcca aagtgagaaa tataatagaa agtaaataaa atcatgtaag
      421 aataatttag ataagattct aagattttat ttatttaaaa tttagtatga ttaatttaga
      481 ttaaataaat ttaataattt tagtaggtgg taatgaatga aaataaatag attaacagaa
      541 ataatagtaa ttctattaaa taaaaaacta gtaacagcta aagagttggc agatagattt
      601 gaagtttcta caagaaccat atatagagat atagaaactc tgtctatgtc aggagtacca
      661 gtatatatga cgaaaggcaa aggaggaggg atatctttaa tagaagaata ttctatagac
      721 aagaccatat tgtcaaaaaa agataaagag agtttaatag tggcactgaa aacattacag
      781 gcgactaagt atccagaaat aaattcagta gtaaataaaa ttggctcaat atttggagag
      841 cagaattttt caaactggat tgaaatagac tttactgaat ggggaagtaa ttttaatgaa
      901 gatgataaat ttacaaaaat aaaagaagct attttaaggt gtaatactat aaattttaat
      961 tatgtaaata gtttgtcaag ccagacaaat agaactgttg aaccactgaa gcttatgtat
     1021 aagagtaaaa cttggtactt gtatggattt tgtaaactaa aagatgattt taggatattt
     1081 agaattagta ggataaggaa tttatctata aaggatgaag tattttcaag aaagataata
     1141 gaagaagcat gtttgaatga ttctaaagta attaaggaaa atactataac gttgaaattg
     1201 aggtttaaag aaaagatgct ttttagagta tttgatgatt ttaataagga tttaataacc
     1261 aaaaatgaag atgatacata tgatgtaatt acggaatttc ctattggtga atggatttat
     1321 ggatatattt tatcttttgg tgacaatgta gaagtacttg aacctaaaga tgttagggat
     1381 aatgtaataa ctagactaag ggagttgtct aaaatttatt cattataata taatgctaga
     1441 ttaaagattt tattaaatat ataatatctc gtattgagta tataatatct cactgagttt
     1501 aattatattg aaaatatgac ataaaggtgt cgtatattat aaattataat tgaattagtt
     1561 caaaataaaa tatattaata aaaacaaagt gaggtataaa gtatgaatta tgaaatagta
     1621 gagataagtg agaaaatagt tataggaata tcgaaggaga ctacaaataa agatggacaa
     1681 gcagtaaatg atataggtga attgtggaaa aaatttatgg gcaaagggat atataatgcc
     1741 ataaagggta agaagagtga caagacaata ggtctatata cagattacca gggtgatttt
     1801 acaagtccgt ataattttgt tgcatgttgt gaagtagata gtaattctga taaagagaag
     1861 aatttagaag aattaaacac taataatact gttggagaaa gtattattag taaagttata
     1921 ccagcaggta agtatgcaaa gtttgttata gtaggaggtc aaaaagaagt aggagatttt
     1981 tggtttgagt tttggcaaat ggattttgat aggacttata taagtgattt tgaagagtat
     2041 caaggcaata cttttgatac aaataaacaa gaaattcata tttacatagg aataaagtag
     2101 attcgttatt actatattgg atgaagtatt gagtatagta gatttattgt agatagtttt
     2161 attatttata actagaagca gtaactctgt aaaagattta ctgctaagat tgttgactta
     2221 tgattttttg atttaataaa atcaagaaga tatttgaatt ttatagtagt tttcattaag
     2281 tgctaaaaac agcacatact tttgttgatg tatgtgctga acatttatgg tgttttgtat
     2341 ttcactcaaa tatataaagt gcttataaca tattatagat taaacccaaa acgcccatct
     2401 tacattgaac atgtcaataa atctaacgac acatgaactg taatcagtag actgcattgg
     2461 ctcaattgtg gtactatcgt ttttcattgt attgtatgct tttagtaggt cagtttcatt
     2521 atcaaattga acacagagct gatatcctcc agatattgaa attccatctt tattcccaaa
     2581 atcgtttaat attagcaatt cattatgaat aaatatttca gcatgaacaa caagtaaatt
     2641 acttgaatct tcaattaatg ttttaacaat ggcattaaac gcttgcttat ataattctat
     2701 tgcttgtttg cattgtccat taaagtaaat atgagtcaat aacaaaacta taccccctaa
     2761 gctaatataa attttttata ctacacatta tccgactaat gattatctgt attataacat
     2821 gattaaattt aaaaagccat caacacatag aatgtcaacg gtttgagcag taaatctgta
     2881 aaagatttac tgctttttta gtaattaata gcatcagtat cactttgaat ctttagacat
     2941 attcttcatt taatattatt tattgataaa tgatacttgt aaatataatg atttgttatt
     3001 taatttaaaa tatgaaaaga aaagtattat aaacacatta gaacacttct ggatttatga
     3061 gtatacaatt tgaaaaatat aaaaaataat gtataaagct aaatctaatt taatcgataa
     3121 tacatataga gaaatataat taagtaaata aacaactatc tattagatat tgagaaagtc
     3181 aaatcctata taaatctaca gaaatgattt taatgaatct gaaaattatt tttatacaaa
     3241 cataaaaaag gtataaagct gttttattat aatttgatta cttttgataa ctttttatgt
     3301 tttatacaat tatattttta ataactcgtc aaaatgccaa acatatatta caatagatat
     3361 ataagttttt ttgttgacaa attatgttaa gagaattaag tataatatga cataaataat
     3421 tacttttatt tatcaatgaa atgtatttat tgtgtagcga aaaatagtca gatagtatag
     3481 acaataactg aaaataaaag cgtttattgt tgtacatatg gaggagttag ttttatggaa
     3541 aataaaattt tgatagtaga tgatgctatt tttatgagaa tgataattaa agaagctctg
     3601 agtaaaagtg gctataataa tttaatagaa gcaaatgatg gagaagaagc gtgtaatata
     3661 tttaaatatg aaaagcctga tttgatgttg cttgacatta caatgccaaa aaaagatggt
     3721 ttagaggtgc taagagaaat aaaaaagttg gattcaagtg ctaaggttgt aatgtgctct
     3781 gcaataggac aagagaatat gattgtagaa gccataaaac ttggagcgtt agattttata
     3841 gtgaagccct tcaaaacaga aacattagtt aaagttgtaa ataatgtttt aaaataaaag
     3901 cgtaaatata cactgataca atgaaaagta aataaatgca ataataaagg gaggggttta
     3961 tattataaat tatttagaac ttgatgatgt tcatatagat gctcttagag aaattggtaa
     4021 tataggctct gggaatgcaa ttactgcact tgcatccatg ataaatagta atgtagaagt
     4081 attaatacct atggtcaaga tacttgaata taatgaagcg acaaatttgc ttggtggtcc
     4141 agaaaataaa gtagtatgta ttttactcga tatgaaaggt gatatcaatg gaatgtttat
     4201 gtttttgttg gatgaatcaa taactcaact tatgctaagt tctcttttta ataaagaaga
     4261 atctacttta gatgagattg aggcaattga aatttcagca attaaagaaa taggaaatat
     4321 tatggcaagt tcatatgtaa atgctattgc atctatgtta aacatgacta taagtgtttc
     4381 aattccagat atatgtattg atatggttgg agctgttcta aatgtgccta tgataagatt
     4441 ttcagatgtt ggtgacaaag tcttatttat agaaaataaa tttaaaatga gtgataatta
     4501 ttttactagt cacattttga tgattccaga aatgagctca cttagagaaa tactcgtgag
     4561 gttgggatta gaagtatgaa tagagagata gtagtgaaca ttgcagatat gaagatagcc
     4621 tatagaccaa atgttttagt tacttatgca cttggttctt gtgtaggagt atgtttgata
     4681 gataaagtag ctggaattgg tggaatgtta cacgttatgt tgccatatag taaagatgct
     4741 attaacattg aaaataagtg taaatttgct gacacaggaa taaatgagct cattaaatct
     4801 atggagaata taggtgcaaa tagaatatat ataaaagcaa aaattgctgg aggggctcag
     4861 atgtttttag gaagtagcaa ttcagtaatt gcaagtatag gacataggaa tgttgaggca
     4921 gtaaaaaaaa ctcttttagg attaaatatt ccaatattag ctgaggacac aggtatgaat
     4981 tatggtagaa caatacgttt ttatactgag acgggcgaat tacttgtaga ttcaataaat
     5041 aaaggtaaga agaaattata atatatattt catagaaagt gtattttagg agggaggtat
     5101 taaatgagat ttccagatga cccatattta gattatggac taattgatga aggaattaag
     5161 tatctaaagt ttaaagtaga tgagcaagat tttggggttg aacttgaaaa ggtaatagaa
     5221 attactggaa atcaagaaat tatatttatt ccagaactac ctacatattc taaaggcata
     5281 ataaatctaa gaggaaaaat cattccaata atagatatgc gtttgagact taaaaaagac
     5341 gaattaatga ctacaaattg catgtatata gtagtaactg aaataaagga cttagtagtt
     5401 ggatttgtgg ttgatagagt tgatgatata gtaagcctag aaaaaagtaa aatatcacct
     5461 ccaccaagag ttactataga atattcggat tattttgtaa gtggagttgg agaattagaa
     5521 ggtaaaataa ttactttact agatttagag aaacttttga cagataaaga tgaaatttta
     5581 atagatgaat taatcaatgg gtttgattgc actatcatgt aatataggga tatagttgga
     5641 atgttattat aaaaagattg tgatgagaac atttaagtat atagtggtat aattattaag
     5701 tataaaaatt ttaaatcatt aaaatttgaa tatattatgt ttaagagagg tttgtatatg
     5761 aaaaataagc aacagggaac aattttagtc gttgatgata gtgttctgat gtgtgactta
     5821 atagataaaa cacttacaaa agataatttt aaggttaaaa aagcatttaa tgcaatagag
     5881 gcaaaagaat ttattgaaga atttatacca gacattattt tgcttgatat aattttacca
     5941 gataactctg gttttgagtt ttgtaaggaa gtaaaatcta atgctcgtac agcagatata
     6001 cctataattt ttataacttc aaaaaataca gatacagaca tagtaaaagg atttggtgta
     6061 ggagcaattg actatatggt gaaacctttt agtatggcag aattaaaagc tcgtgttata
     6121 gctcatcttg aagtaaagaa atctcaagat aaattaaaaa agataaataa tgaactggaa
     6181 tcttctcttg aaaaattgaa tagactcgtt gtaagagatt atcttacagg tctttacaat
     6241 aggcgacata taattaatca gcttatggag caaagaagaa ttaataaata tagtgaaaca
     6301 tcaatatccc ttattcttgg tgatattgat gactttaagg ttatcaatga tacttatggg
     6361 catgaagcag gggatttcgt gttaacagta atatcaagta taataaaaaa gaattgtaga
     6421 caaattgaca ctgtatcaag atggggtgga gaagagtttt tgataatgct aacaaatact
     6481 ccaatagaag gtgctaagat actttcagaa agaatccgta aagagataaa ggagtttgat
     6541 ttcaactata aagaaaataa aataaagtgc actattacat taggagtagt agaagtaaac
     6601 tcaaatatat cattagaaga aaacatatcc ttagcagaca aagcaatgta tgagggtaaa
     6661 aacttaggaa aagattgtag tgtagtgagt acaatgaaag gattaaaaaa gatataaact
     6721 tactttataa aggagtacag atatgaaaag agaagtaaag ctttctaaat tagcaattgt
     6781 attgctgatt attatagtat tggctgttct agcagggatt ttttcttcta tttcatctta
     6841 taaagcattt gaaaaagtag aaaaaagtat taatgatatg aataatttga gaactcaaag
     6901 tcagtatata aaagatacaa ctagagaaat aagtgatgta ttaagaagat acgtatttac
     6961 aggagaaaag aagaataaag atatttatga aaaagaatta tacggaaaaa caagagaatc
     7021 agctttaaac aaattagaaa aaattggtct tacaaagagc gagtcaaata aaatcttaga
     7081 agcaaaagaa atatcagatt ctttagtacc aacagaacaa agtgcaataa aggcagtcga
     7141 aagtaatgat aagagtaaag ctcaaagtat aatttttggt agtgactata taaactccca
     7201 agagaaaatg gattctttaa taaatgaatt tcaagaggat atagttaccc gttcaaattc
     7261 caaaattgta gaagtaaaaa aagaattaca aagtctaata ttaagaacaa tatttatact
     7321 ttctctttta ataatattga caataataga atatacagta ataaaattaa aaatagtgat
     7381 tcctattaat aaagtagaaa aacacttttc aagaattgca tctggggact taagaacaag
     7441 tatagacgtt gaagaaagta aatctgaaat agggctatta gtgagttctg tcaaaaagat
     7501 gcaaagtatg cttttaaatt acatagacct tatagacagt acattaacta gtattgcaaa
     7561 aggagattta agagtagaga tagaccaaga atttataggt gattttaaat caattaaaat
     7621 atctttagaa agtataataa actcttttaa tggtgatttt tctgaaataa atttatcagc
     7681 agagcaagtt gcaagtgcat cagaacaggt tgcagcaggt gcacaagctc tttcacaggg
     7741 tgctactgaa caagctagtt cagtagaaga attagcttcc acgattaatg agatatcaga
     7801 tggaattaag aagtgtgcag atagtgctag tcttgcatgt gaggtatctg accaatctgc
     7861 atcacaagtt ggttttggaa gtagatgtat ggatgatatg atgacatcaa tgggtgaaat
     7921 aagtgaaaaa tcgagtgaaa tatcaaaaat aatcaaaaca atagatgaga tagcattcca
     7981 aactaatatt ctcgcattaa atgcagctgt tgaagcagca agagcaggtc aatatggtag
     8041 gggatttgca gttgtagcag atgaagttag gtctttggct ggtaaaagtg caaaagcagc
     8101 tcaaaataca gctattctca tagaagaagc tattaaaaca gtagaaaatg gtgtcagaat
     8161 tgctaaagag actgcaaatg cattggactt ggtagtaaat ggtgtagaaa aaactactaa
     8221 ttatattaat ggtatttctt cggctgtaca tagtcaagaa gtagcaatta cacagatttt
     8281 atctggaata gagcaaattt ccatagtagt ccaaatgaac tcagcaacag cagaggaaag
     8341 tgcagcagcc tcagaagaga tgtctgctca agctcaaatc ttaaaagaat tagtagaaaa
     8401 atttcatcta aaggaaagtg aagtaagaga atcatcatgg gtttaaatta tgagatgcat
     8461 atatatagta gaataagaga atgattgtgt atttaaatta tgaggtacat atagtgagat
     8521 aagagaatca tcaaggattt aaattatgag atgcatatat aatagaaaat tatttgtagg
     8581 tcgtaggtga taaatgtgaa ttatattgaa ccgagtatga gacaaatgtt agatatgttt
     8641 atatttgaga caagtctatt acttgaacaa ttagatgaaa ttatgattca aatagaaaga
     8701 gagaaagtat ttactcataa caacattaat gaaactttta gaataatgca tacgataaaa
     8761 ggttcagcat ctatgatggg gttagacaat atatctgctt tagcacatag tgtagaagac
     8821 ttgttttatg ctatacgaga aggtaaaata gaagttttag acaatgacct attatttgat
     8881 ttagtctttc aatctttaga ttttataaga agagatatag atgagttaca atcaagaggt
     8941 tgtatatctt ctgattactc taattttatt gaaggaattg aagagtatgt gagtactttg
     9001 gaattatcaa gttctatgaa atcaaaggat aatgcaaatt tagaaaatac agacgaaaaa
     9061 gaagtaatca attcaaattt tgcatacaaa gaagactctg atgtagtaaa aataatcttt
     9121 gaacaagatt gcatgatgga aaatgtgaga gctttcatgc ttgtatttga acttagagat
     9181 tactgtgaat ttatagattt ttatccaaaa gatgtagaaa caaatgcaga gtctacagaa
     9241 tatattaaaa gttatgggtt ttatatacaa tttgtagcta aagagaataa ggaaatagtt
     9301 tataaaacta tagaagataa tctctatgta aaagaatata taaatattag taaaaaagaa
     9361 atagaaatga aaagtacatc aaacaatgat gcagtagaaa ataaattaaa agataataat
     9421 aaagtaagta aaggttatca aaataactat acagaagatg agttacaaga agatgtttta
     9481 aatgaaaaaa ttgatataga aaaagatagt ctaagtgaag aaaatgaaca tttaccaata
     9541 agtaagcaga gtattatgag tgtaaattta gaaaaattgg acttacttca aaatatagtt
     9601 ggagaaattg taattatgga atcaatggtt gtaaatagcg taaattctac aggtgttgat
     9661 atagataatt tcaataaggc aacaagacaa ttacacaagc ttacagatga attgcaagat
     9721 atagtaatgt ctatgcgtat gattcaaatt gctggtgtat ttcaaaaaat gcatagaatt
     9781 gtacgagata tgaataaaaa attagataaa aatgtagagt taataactat aggagcagag
     9841 acagaggtag ataagagtgt aattgataat ttagcggaac cttttatgca ccttataaga
     9901 aatgctatgg accatgcaat agagcctatt gaggagcgta tagcgaaagg taaagaagaa
     9961 gtgggaaaaa tagttttatc tgctgagaat ttaggcggag aagctgtgat aagtatttca
    10021 gatgatggta gaggcattat gagagataag attttggcaa aagctaaaga acaaggtctt
    10081 cttaaacatc aaagtaatga gtatactgat gacgaggtca attctttgat tatgactcca
    10141 ggtttttcta caaaagaatc tataactgaa ttttctggaa gaggagtagg tatggatatt
    10201 gttcgtaaaa gtattgaaaa ggttggaggt tcaattgaag taaaaagtaa acaggatgaa
    10261 gggacaacct ttactataaa aataccatta actctttcta tagtagaggg tatgaagttt
    10321 aaagttggag attcactatt tattttacca atatcatcaa taagaagaac atttaaactt
    10381 acagacttaa atcaaataat cacagaggga aataaaacag agttaattac aatatatgga
    10441 gcatgtattc ccattataag gttacataaa atatataata ttcaaacaaa agtaaccaat
    10501 ctgatgcatg gtaatatgat aattattgaa aatgagcata aagcagtatg catttttgtt
    10561 gatgaaataa taggtaatca acaagttgtt gtgaaaccat ttccagttta ttttaaccga
    10621 tttaatataa aaaataatgg tttatcggga tgtacaatac ttggggatgg aagtataagc
    10681 ttaattatag atgcagatag cattatagaa atatattagg ggattttaaa gtatggctaa
    10741 agatgaaatt attaaacaag tattaacatt ttatgtcaat gatgttattt atggaataga
    10801 attagaaaat gtgattgaga ccataagatt tcaatcaata acgtatgtac catgtcttcc
    10861 aacatatata agtggagtta ttaatttgag aggaagaata ataccagtta ttaatatgca
    10921 tataaagtac aatcttccta aagctgatta taatgagaga acttgtataa taattacaaa
    10981 ggttgatgag tatcaagttg gaattattgt agataaagtt gttgatgtta ttactataga
    11041 aaatctaaat ttactagaga cgactaattc taacaacact aatattaata aagatataaa
    11101 agagattgcg aaagttgaag ataattctat attaatttta gatattcgaa agtttttgat
    11161 taatagcatg tagttagttc tgataaatta gcaggaggaa gtttttgcat gataaaactc
    11221 acagatgaag aatttatagt tcttgtaaac catgtaaaaa aagaatatgg tattgattta
    11281 agtaaaaaaa gagcgttaat tgaaggtcgc ttatacaata caatgataga gaaaaaactt
    11341 agttctttta gtcaatatat gaatctatta tttaaagata agacaggaaa tgaagctata
    11401 aacctaataa ataggttgag tactaaccac acgtttttta tgagagaacc tcaacatttt
    11461 gagtttatac agaatagtat acttcctttt tgggaagaaa ataacaaaat aagaaacata
    11521 aatatatgga gtgcaggatg ttctagtgga gaagaagcgt attcaatagc tatgactttg
    11581 gatgattatt ttggatataa taaagagttg tggaacatta aaatcatagc cacagatatt
    11641 tcaactaact cacttgaaaa agctaaaaga ggtatataca tagaatcaaa tgttaacaat
    11701 gttcctgagt tatggaagaa gaagtatttc atagacaata aggatggtac atttaaaatt
    11761 tgtgataaga ttagaaaaag tgtaattttt aagccattta atttaatgaa tgatttttca
    11821 tatcaacatt ttgatttgat tttttgtaga aatgttatga tttattttga tttaaaaaca
    11881 agagaagaac ttataaataa attttataat gctaccaaaa aagatagttt tttgtttgta
    11941 ggacatgcag aagtaattaa cagaagtagt actaaataca aatatgtaaa acctgctata
    12001 tataaaagat aaaaaggtat ttttgataga tgattttttg gaggcggtaa tgtggaacgt
    12061 tttaataata tggttattgc tataggcgca tctgtaggtg gtacagaagc aatacttgaa
    12121 attataaaag acctaccaaa atcaacacca gggattgttg tagttcaaca tatgccagct
    12181 atatttactt atatgtatgc acaacggctt gacaagcagt gtattatgaa tgtaagagaa
    12241 gctaagaata atgatagagt cgaacaagga aatgtgttaa ttgcacctgg gggttaccag
    12301 atgaaattgt gtacggataa acaaggatat tatgtgactt gtgaaaaagg agaaagaata
    12361 agtggtcact gtccatcagt agacgttttg tttgattcag tagcagaagt agcaggtaaa
    12421 aattcaatag gaataatact tacaggtatg ggttatgatg gagcaaatgg acttttaaaa
    12481 atgaaagaaa atggttcatt tacaattgga caggatgaaa attcttgtat agtatatgga
    12541 atgccaatga tagcttttga cagaggttct gttatgttac aattacctct tggtgaaatt
    12601 tcaaactgtt tgataagaca tattaatatt ttaaaacaaa aagattaatt ttttataaat
    12661 aaaaattaat cttttattaa attatatgga taaaaaaccc tagaaactcc aaatataata
    12721 agagagattt atatttagga ggaaaatgat aatatgaaat tttttaaagt cgatacaaaa
    12781 gctagagttt taagatatgt atttgtttta acattgacta taatggctgt tttggcaatt
    12841 aaacatgact ataaatataa taatttaagg aaagatgctt caattaattt aaatagtaat
    12901 aaggaagcag tgactgttat gagtaaccag tcattaccaa agggtaatac aaatagtaaa
    12961 caaactttat cagtaagtac aataggaagt aaaacaatag tgaaaaatga tgagtattta
    13021 gaaagtacta taaacatgcc tgtaataaca aattcaaata aaattgttga aaggtctaca
    13081 aatgataaga taaaaaatga tatatttgaa ttttataata aatcatataa agaagcaaag
    13141 cagtatctta aggataatcc tgatgagaaa aataagtttg ttgcaaatgt agattttgaa
    13201 cttaaaaaga atactgattc tgctcttagt ataaaagtga gatattatac ttatagtggt
    13261 ggagcacatg gattttatca agatatagca tataatgtgg atatgagaac tggaaaattt
    13321 ttagaattaa tggacttatt taaggataat actaaatata cggaagttat agacgaagaa
    13381 attaaaagac aaatagctga acttgaaaaa aaggatgaag aaaatattgg aatatataat
    13441 tttaaaggta taaaagagaa tcaaaacttt tacttgcaag atgagaatct agttgtatat
    13501 tttgatttat atgatataac tccatatgca gcaggaatac cagaattttc aataaataaa
    13561 gcgttgataa gttccatgct aaaaccagag tatgtagatt tatttgattt gaaatagaag
    13621 attaaattaa atgttatgct ataatattta gtatagagac ttattaaaat tgggggtata
    13681 aaaatgctac ttttaaaaaa agacgacata gaaaaagtat ttacaatgag agatgcaatt
    13741 gaagctgata aggaagcttt tagaatttat tgtgaaggaa agagtgtaaa tcctttgaga
    13801 accaatatat ctgttccaag tcaagatgct agtatgttat ttatgccagg atacgtagaa
    13861 gaattaggct gtgggggaat aaaaattgta tctgtatttc ctaaaaatgc tcaaaaaggc
    13921 aaaccagtaa ttccagcaac agtactatta ttagatggag aaactggaga agttagtgca
    13981 gtgttggatg gtacttatgt aactcagatt cgtactggag cagcatctgg agcagctata
    14041 gatgtattgg ctaaaaaaga ttctaagatt ggagctttga taggtctagg aggacaaggt
    14101 gagccacaac ttgaagctat gatagaagct agaaatttgg atgaggtaag agtttttagt
    14161 agaaacaagg aaagtagaga aaaatttgca gaagaaatga acttaaaatt aagtaaatat
    14221 aatactaaaa tagttgcagt tgaatcatct gataaagcta tagataatgc agatgtgata
    14281 gttttagcta ctccatcaaa acaaccagta tttaatggaa acttagtgaa aaaaggagct
    14341 ttaataagtg cagtaggttc atatatgccg gacatgcaag agttgtgccc aaactgttta
    14401 actagagcat ctaagatatt ttttgaatca acagatgcag ttttatctga gtcaggagat
    14461 atattaattc cattaaaaga aggtaagata agtaaagatg attttagtgg tgatttaggg
    14521 aatgttataa acggaactat tcctggaaga gaagatgatg atgagataat tgtgtttaaa
    14581 actgtcggaa taggtgtaca agatgtcgta acagcaaaaa gaatatacga caaagcaaaa
    14641 gaaaatggta tcggaatgga ctggaagtgg taaaatattc aaaataattc tatagttaga
    14701 caaatttatg ttataatgtt gataaataaa ttttttatta ttttaattga gggaggatgt
    14761 tttatgaaaa tggtaaaaaa gttaatatta tcttcgatta tggctctagt tttagttcta
    14821 actatagggt gttctaatgc agaagaaaaa gtaaagtcga attttgaagc taatttagct
    14881 gcattagaaa tgtataagta tgatgaaaaa ggaatttctg atgcattaac agaagctgaa
    14941 tataaacaga ttcaaactta taacagcaag gataaggttt caatgattgc tcagttgaca
    15001 cctctttatg gtaaagaaaa tgctgaaaag atatacgaaa caattgttaa attaattcaa
    15061 ggtgttgaaa agacagtaac agtggaatct tctagtaagg ataagataac tttgaaagtt
    15121 gtaagtaaag ctttagatac tagtaaagta atgagtgaag cgaacacttt agtaaaatct
    15181 tatgtaactg atgaaaagaa ttacgataaa aaagaaaaag aagttcaaga tgatatgaca
    15241 aaaatgatta tagaccttta caataagaaa ccaactaagg aagttacaac tatgattaca
    15301 tatactaaaa aagatggtaa atggcaacca cctagtaata tacaaaagtt atgtgaagga
    15361 atatatacaa tttaatttat tttaaagaca caaaactatc ttttaagcat ata
//
LOCUS       DAEPXF010000088        15253 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain HB27
            SAMN13376389-rid12479423.denovo.093, whole genome shotgun sequence.
ACCESSION   DAEPXF010000088 DAEPXF010000000
VERSION     DAEPXF010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13376389
            Sequence Read Archive: SRR10518432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 15253)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15253)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001069624.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001069624.1
            Genome Coverage       :: 315x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/15/2021 01:54:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,165
            CDSs (total)                      :: 4,076
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 89
            rRNAs                             :: 10, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 10 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 19 of 69
            Pseudo Genes (incomplete)         :: 44 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 10 of 69
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15253
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13376389-rid12479423.denovo.093"
                     /strain="HB27"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="China: Hebei"
                     /collection_date="2017"
     gene            63..1205
                     /locus_tag="KRL67_002338"
     CDS             63..1205
                     /locus_tag="KRL67_002338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HBH1523212.1"
                     /translation="MRYVEYGKTGKMISVVGYGGLRFDLEKSNQENADLVKYAYEKGI
                     NYFDTAPGYCDDRSEDIFGLAFRQMIKEGKTDFYVSTKEKPKACDTSEKAIEAVKKSI
                     ERLGVSKINFYHVWNIRKMEHYELAMKPGGQYEGLLKCKEEGLIDHIVFSSHQPGDEV
                     IDILSENKFEGVTMGINILNFPYRLKGAKYAVDNGYGVVAMNPLSGGTIPKYNKELSF
                     LSKDGESTIESALRFNIGISQISISLIGFNKKQDIDDACKIADENRIYSDEDIHDIET
                     RLNKNMNEICTGCGYCKVCPKGINTPAYMLFYNEKQMFKKSDEEMTKLVYGLGHWNYT
                     MNSKAKAKECISCGKCEVECTQHLPIIDRLKEIKKWEEDGANSVKA"
     gene            1604..2842
                     /locus_tag="KRL67_002339"
     CDS             1604..2842
                     /locus_tag="KRL67_002339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH1523213.1"
                     /translation="MDGDILMSKILKYKNEMFLFILVVITYLIITKIFFSKTTIFYDL
                     NNTYDVLLDTDTGVLFNLNVFAISQDNSKHILFSAIISIFAYPIYLFCTSIANPGTTD
                     FNSAYGFGLICLQIITSAMSITLVFNHIKKIKMQRLTLILLTMIMIFSFPQLFMTLNV
                     ERFIYSQFSLIFFIVIANKMKGKNSYLVELAAIPLFGITISNIYLYFFNMIFEFKLKI
                     GKMLKHLITFILMAYICVVSTKSYESFMNLGNVIQYDTKFISGEPILEKIAMIIERLL
                     YSIFYFPGASIKKGLFLQNGEVATIPVILTLLALCFCVLSVIENSEKRVPKLCMGIII
                     FNLTLHGIVGYNLVNSSIMAINFSFAVIILLAYFTKALRKNEKNMYNIFLSLLLVTIV
                     ISNINGFIEILNIGIKSYPV"
     gene            2871..3758
                     /locus_tag="KRL67_002340"
     CDS             2871..3758
                     /locus_tag="KRL67_002340"
                     /EC_number="2.4.2.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="decaprenyl-phosphate phosphoribosyltransferase"
                     /protein_id="HBH1523214.1"
                     /translation="MKQTNTSIRLLTKISDYIKLMRIKQYIKNFFVFSAIIFSNNILN
                     INLFLNTFIAFVCFCLMSSSVYALNDSIDMDKDKKHPKKCNRPVASGRISKKSANILF
                     VVLALISVCLSTVVSFNLSIILSLYLINNILYSLKLKNIILLDVFSISLGFILRVYAG
                     CVAISVSLSNWIILCTFFLSLYLALGKRKKEIETLRDDAVEHRKILEDYDIENLNQMM
                     IVILSSTIVCYALYSTSNPEKPHMIFTTIFVVYGVLRYNYIINTTNENNPTDIVLKDN
                     ALKINVILWIITCLIILIF"
     gene            3792..4421
                     /locus_tag="KRL67_002341"
     CDS             3792..4421
                     /locus_tag="KRL67_002341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IB family hydrolase"
                     /protein_id="HBH1523215.1"
                     /translation="MKKSVLLLDVDYTVINTDSMIDFFIYSLKNKTFKTIIKLPYIIF
                     ILFMYLIRMIPLRKAKEAIFYPIVDFSEEDLKTFFDNCIMKKINESIKKVIYKNKEED
                     NVIIMITASPYAYMKYFKYYGLADEVIGTEFFYENSRYKNQFIGENCKGIEKVKRIKA
                     VLGKLGIEIDYENSYAYSDSKSDLPMLSLTKNAFLVSKKDGEVIEQINS"
     gene            4426..4890
                     /locus_tag="KRL67_002342"
     CDS             4426..4890
                     /locus_tag="KRL67_002342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small multi-drug export protein"
                     /protein_id="HBH1523216.1"
                     /translation="MSLRIGGNSLGYLHILILSMVPVTELRGAIPIGIAMGLNPIWVY
                     VFSVIGSTLVSIPLILTFRHVLQFLRGKKLFKGIADVIDRKINSRMKKLKSVSIIGII
                     LFVGIPLPTTGTWTASAIASILKMRIKDAFMGVFLGNLLSGVIVSALSLHII"
     gene            5140..6507
                     /locus_tag="KRL67_002343"
     CDS             5140..6507
                     /locus_tag="KRL67_002343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HBH1523217.1"
                     /translation="MENSNTNLGSESVGKLLFKLATPAIIAQIVNVLYNIVDRIFIGR
                     MENGEVAMAGVGVAFPIIIIITACSYLIGMGGGPLAAIKMGEQNNDEAEKIMSNSFSV
                     LVILAILLTIGFKIGKEPLLWMFGASESTIGYSMDYLNIYLIGTVFVQISMGMNTFIN
                     TQGFATTGMMTVAIGALINIILDPIFIFGFNMGVKGAALATIIAQGVSAIWVLMFLFG
                     KKSILKIKKKYMIPKASIILPVLGLGISPFIMQSTESLVLIALNSKLQMYGGDLAVGS
                     MAIMSSIMQILMLPNMGVTQGAQPIISYNYGSGQLDRVKKTFKLCLLSCFTYSTILWL
                     LLMIFPAFFVSIFNKNPQLLSMTSWSIKIYFAGAFMFGIQIACQQTFLALGKATISLV
                     LALLRKIVLLIPLIFILPTFFNEKLFAVILAEPVADITAATITAISFFIFYKCFLSKP
                     KAIKE"
     gene            6707..7981
                     /locus_tag="KRL67_002344"
     CDS             6707..7981
                     /locus_tag="KRL67_002344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HBH1523218.1"
                     /translation="MFIMENDLTMQELLDQQEQVFSKVKVGELTTGKITAVRNDEVQL
                     GLDYGFDGIIPISELNIEKNQYIEDIYHIGDEITAVITKVSQKDGTITLSKLQLDKRN
                     DFAELQKAYDEHRIITVNVEKNIDKGVFANYNTYTFFIPISQLDTKFITDTSKFVGLN
                     LEVYIKELDVRKNRLVASHRDVLQERINKEREERRAQIKAEKEAERARIKQEREEEKA
                     RIKAAKEDLFNSLEVGQKRDGKVTKIMPYGAFVDIGGIEGLAHINNLAWTRVESVEDV
                     VSEGQEVEVYVLDVNKETKKIALALKDINNDPWDLIAKEVQIDDVVNAKVLRIIEKGA
                     FVQIKEGVDAYLPISELSDTRVAKVTNVVNIGDEVKVKILDFKPKTKRMLVSIKEATR
                     EPEEDITEYLEVEESLGSIGELFKDKFKDLEV"
     gene            8099..8818
                     /locus_tag="KRL67_002345"
     CDS             8099..8818
                     /locus_tag="KRL67_002345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase"
                     /protein_id="HBH1523219.1"
                     /translation="MKKSIMDVHCHTLISGHAHSTFKENVEEASKKNIKYLGISDHGP
                     NMPGGPHPFYFYNLHLLPREVQGVKILRGIEGNIMDYSGNLDIQEDMLQHLDYIIASL
                     HRPCIASGTKEENTNAILKVMDKPKVKIIGHPDDSRYPLDYEPIVKKAKDKNILLEIN
                     NSSLSSNSHRTGTWENVSHMLTLCKTYGARVILGTDSHICYSIGEFENAEKVLEAVDF
                     PDELVINYHEDEIIEFFDINF"
     gene            8839..9285
                     /locus_tag="KRL67_002346"
     CDS             8839..9285
                     /locus_tag="KRL67_002346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH1523220.1"
                     /translation="MSKNKFIYMNYYDNGYFKKGMTIYMVLALIILATIFMYSTTILY
                     ILCIITAVFLFKQGRDFSNKYEGKVYITLDNHSILINNQCIFSIKSKQSQKFNYKIIE
                     KIEVVKNILNIYTDESNYKIRLRALSLEDEKKLLNIIDEKMKKFKA"
     misc_binding    9433..9679
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            9802..11544
                     /gene="leuA"
                     /locus_tag="KRL67_002347"
     CDS             9802..11544
                     /gene="leuA"
                     /locus_tag="KRL67_002347"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HBH1523221.1"
                     /translation="MKCGKYKKYDKMQIVNRKWPDNEIFKAPIWCSVDLRDGNQSLPT
                     PMSVNEKVRMFKMLINTGFKEIEVGFPSASNTEYTFLRKLIDENMIPDDVTIQVLTQS
                     RAHLIEKTFESIKGCKKAIIHLYNSTSVLQRDVVFNMSKQEIIDIAVEGAKLFNEEVK
                     KYPETEFTFEYSPESFTGTEMDYALEICEAVIAVWKPTPQKKVIINLPSTVEMATPNV
                     YADQIEWFCENVSCRDSIILSLHTHNDRGTCTAASELGLLAGADRLEGTLFGNGERTG
                     NMDIVNVGLNLYTQGIDPELDFSNIDKIREIYEDCTKLMVHDRHPYAGNLVHCAFSGS
                     HQDAIRKGMIAMKNRDNDYWEVPYLPIDPHDIGREYKEIIRINSQSGKGGAVYIMETD
                     YGFMIPKNMHSDFGNVVKMESDRIGEELSSEAIFNLFKKEYIEVESPYKVKKYKIKSM
                     DELNYENDDSNDTNMIEMTARISYMGNEQRIVGIGNGPVDSFNNALKQCGMKDYKFRY
                     YWEHALEEGSHSRGVAYVGIEHNNEVYFGVSISENINTAAINALMNAINKSYIEEEIK
                     NGDDYDAENISQTC"
     gene            11501..12778
                     /gene="leuC"
                     /locus_tag="KRL67_002348"
     CDS             11501..12778
                     /gene="leuC"
                     /locus_tag="KRL67_002348"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HBH1523222.1"
                     /translation="MGMTMTQKILAKHAKLNEVKKGQLIEADLDVVLGNDITSPVAIR
                     EFEKLGIEDVYDKTKVVMVLDHFTPNKDIKSAEQCKFTRSFAKSKGVVNFFDVGDMGI
                     EHVLLPEKGIVTAGDVIIGADSHTCTYGALGAFSTGVGSTDMGAGMATGKCWFKVPGA
                     IKFVLKNKPNKWISGKDIILHIIGEIGVDGALYKSMEFCGDGVEYLSMDDRFTICNMA
                     IEAGAKNGIFPVDDKTMEYINSHKCSTITKDVNIYEADEDAVYDEVYEIDLAELKETV
                     AFPHLPENTRTVDDIDKDIKIDQVVIGSCTNGRISDLEVVAEIMKGKKVADGVRVMIF
                     PGTQKVYLEAIEKGYITTFIEAGAAVSTPTCGPCLGGHMGILAAGEKSISTTNRNFVG
                     RMGHVDSEVYLASPAVAAASAITGKISKPSEII"
     gene            12793..13284
                     /gene="leuD"
                     /locus_tag="KRL67_002349"
     CDS             12793..13284
                     /gene="leuD"
                     /locus_tag="KRL67_002349"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HBH1523223.1"
                     /translation="MIANGSVFKFGDNIDTDVIIPARYLNIADYKELATHCMEDIDDN
                     FISKVKEGDIIVATKNFGCGSSREHAPIVIKESGVSCVIASTFARIFFRNSINIGLPI
                     LECEEAANNIDEGDNIEVDFSTGVIKNITKGKEYKAEPFPEFMQNIILNEGLINSIKA
                     NRG"
     gene            13291..14361
                     /gene="leuB"
                     /locus_tag="KRL67_002350"
     CDS             13291..14361
                     /gene="leuB"
                     /locus_tag="KRL67_002350"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HBH1523224.1"
                     /translation="MNCNIAVIKGDGVGPEIIDEGIKVLNKICCKFNHRFDCEYVLAG
                     GCAIDETGEPLPNKTVEICRKNEAVLLGAVGGAKWDKCKGDKRPESGLLKLRESLGLF
                     ANLRPATMYESIKEASPLRTDIVEKGIDFVVVRELTGGIYFGERGRKIIDGIENAYDV
                     EIYNENEIRRIGKRAFEIARNRNKKLISVDKANVLESSRLWRSIMEDLAKEFEDVELS
                     HMYVDNAAMQVVKDPSQFDVIVTNNIFGDIISDEASMITGSIGMLPSASLREDSFGMY
                     EPIHGSAPDIAGKDVVNPIATILSVSMMLRHSFNLEEEAKCIEDAVQSVLNKGYRTID
                     IYNGVGNVVGTREMGKLIVNEI"
     gene            14725..15240
                     /locus_tag="KRL67_002351"
     CDS             14725..15240
                     /locus_tag="KRL67_002351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HBH1523225.1"
                     /translation="MDFLAKYKVLKKQNSSKSCLVCGTQNELGLKADFYELENGELVS
                     ICNTKDWHQSYPGRVHGGMSAAILDETIGRAVSINDDQIWGVTVSLELKYKKPVPTDA
                     TIKVVGRITKENRKLFEGTGEIILPNGDIAVTATGKYMKMPIGQIAEGDFSNEEWFFE
                     ESKEKVEYIEL"
BASE COUNT         5899 a         1550 c         2815 g         4989 t
ORIGIN      
        1 tttatggtat aatattttct ataaagaaat taagtcttaa tatatctaag gaggaattta
       61 caatgagata cgttgaatat ggaaagacag ggaaaatgat ttcagtagtc ggctatggag
      121 gattaaggtt tgatttagaa aaaagtaatc aagaaaatgc tgatttagtc aaatatgcgt
      181 atgaaaaagg tattaattac tttgatacag caccagggta ttgtgatgac agaagtgaag
      241 atatatttgg actagccttt agacaaatga taaaggaagg taagaccgat ttttatgtat
      301 caactaaaga aaagccaaaa gcatgtgata catcagaaaa agctatagag gctgtaaaaa
      361 aatcaataga acgattaggc gtatctaaaa taaattttta tcatgtttgg aatattagaa
      421 agatggaaca ttacgaacta gcaatgaaac ctggaggtca atatgaagga ttattgaaat
      481 gtaaagaaga aggattgata gaccatatag tattttcatc tcatcaacca ggagatgagg
      541 ttatagatat tctaagtgaa aataagtttg aaggtgtaac tatgggaatt aatatattaa
      601 actttccata taggctaaaa ggtgccaagt atgctgttga taatggatat ggtgttgtgg
      661 caatgaatcc attaagtggt ggaactattc caaagtataa taaagaactt tcttttctat
      721 caaaagatgg tgaaagtact atagaatcag ctttaagatt caacatagga atttcacaaa
      781 taagtatatc tctgataggg tttaataaaa aacaagatat agatgatgca tgtaaaattg
      841 cagacgaaaa tagaatttat tcagatgagg atatacatga tattgagact agattaaata
      901 agaatatgaa tgaaatatgt acaggttgtg ggtattgtaa agtatgtcca aaaggtatta
      961 ataccccagc atatatgctt ttttataatg aaaagcagat gtttaaaaag agtgatgaag
     1021 aaatgacaaa gttagtttat ggcttaggac attggaatta tacaatgaat agtaaggcaa
     1081 aagctaaaga atgtatatct tgtggaaaat gtgaagttga atgtactcag catttaccta
     1141 taatagatag attaaaagaa attaaaaaat gggaagaaga tggagctaat agtgttaaag
     1201 cctaattaat tttatactat atttgaaatt tgtatagtat ttagtaatgc tttaaaatat
     1261 agttttaata tttaaggaga cagattataa ttttgtctcc ttaaattatg ctttaatttg
     1321 ttatagttta attccataag caacgttgta tatttttata taaagttttt caaatatttt
     1381 acattaatat tgcctacatt aatattgctt acaaattctc attttataca acactaatct
     1441 atgttatata aacaatactt gactaattat aacatcaata aagtaaatat tgtggttgta
     1501 aataaagagt tattcaatta caataaaaca agtaagaaca ttatgaagat ggctattaaa
     1561 tataaggaat aaattgtagg tgtcagcaaa tcctatataa tgaatggatg gtgatatatt
     1621 gatgagtaaa atattaaaat ataaaaatga gatgtttttg tttatattag ttgtaataac
     1681 ttacttaata ataactaaga tatttttttc taagactact atattttatg atttaaacaa
     1741 tacttatgat gtattgttag atacagatac aggggtttta ttcaatttaa atgtatttgc
     1801 tataagtcaa gacaatagta agcacatatt gttttcagca attatatcaa tttttgcata
     1861 tccaatatac ctattttgta cttcaatagc aaatcctggg acaacagatt ttaatagtgc
     1921 ttatgggttt ggactaattt gtctacaaat aataactagt gctatgtcaa taactttagt
     1981 ctttaatcat attaaaaaga taaaaatgca gagactaact ttaattttac ttactatgat
     2041 aatgatattt tcttttccac agttatttat gactttaaat gtagagagat ttatatatag
     2101 tcaattttca ttaatatttt tcatagttat tgcaaataag atgaaaggaa aaaattctta
     2161 tttagtagaa ttagcagcta ttcctctatt tggaataact attagtaata tatatttgta
     2221 tttttttaat atgatatttg agtttaaatt aaaaatagga aaaatgttaa aacatttaat
     2281 tacatttatc ctaatggcat atatttgtgt agtttcaacg aaatcttatg agagttttat
     2341 gaaccttgga aatgtaatac agtatgatac gaaatttata agtggagaac caattttaga
     2401 aaagattgca atgattatag agagactgtt gtattcgatt ttttattttc ctggagcaag
     2461 tattaaaaaa gggttatttt tgcaaaatgg agaagttgct acaataccag tgattcttac
     2521 attattagct ttatgttttt gtgttttatc agtcattgaa aattcagaaa aaagagttcc
     2581 taagctatgt atgggaataa ttatattcaa tctaacttta catggtatag taggatataa
     2641 tctagtcaat tcaagtatta tggctataaa ttttagtttt gcagttataa ttttattggc
     2701 ttactttacg aaagcgctta gaaaaaatga aaagaatatg tataatatat ttctatcgct
     2761 gttattagtt actatagtaa ttagtaatat caatggattt atagaaattt taaacatagg
     2821 gataaaatca tatcctgttt aaaaaataat attgaaaggg tagtaagttt atgaaacaga
     2881 ccaatacaag tataaggctt cttactaaaa tttcagatta tataaagtta atgagaataa
     2941 agcaatatat aaagaatttc tttgtatttt cagctataat tttttcaaac aacatactaa
     3001 atataaattt atttttaaat acgtttattg cgtttgtgtg tttttgccta atgtcatcat
     3061 cagtatatgc gctgaatgat tctatagaca tggataaaga taaaaaacat cctaaaaaat
     3121 gtaatagacc agtggcaagt ggaagaataa gcaaaaaaag tgctaatata ctatttgtag
     3181 tattagcttt aatttcagtc tgtttatcaa cagttgttag ttttaatctt tctataattc
     3241 tcagtctcta tttgataaac aatatattat attctcttaa actaaagaat ataatactat
     3301 tagatgtgtt ttccatatct cttggtttta tacttagggt ttatgcagga tgtgtagcta
     3361 taagtgttag tttatcaaat tggataattt tatgtacatt tttcttatct ctgtacttag
     3421 ctcttggaaa acgtaaaaaa gaaatagaaa ctctaaggga tgatgcagtt gaacatagaa
     3481 aaatattgga agattatgac atagaaaatc taaatcaaat gatgatagtt atattaagtt
     3541 ctacaattgt atgttatgca ttgtattcta caagtaatcc agaaaagcca catatgatat
     3601 ttacaacaat atttgtagtt tatggtgtac ttagatataa ttatataatt aacactacaa
     3661 atgaaaataa tcctactgat attgtcctaa aggataatgc tttaaaaata aacgtaatct
     3721 tatggataat tacatgttta ataatattga ttttttagtg atatttttag tgataggatg
     3781 gggaaatact tatgaaaaaa agtgtattat tacttgatgt agattatact gttataaata
     3841 ctgatagcat gatagatttt tttatttact ctttaaaaaa taaaaccttt aaaactatta
     3901 taaagttgcc atacattatt tttattctat ttatgtattt aattagaatg atacccttga
     3961 ggaaggcaaa agaggcaatc ttttatccaa tagtagattt tagtgaagaa gatttaaaaa
     4021 ctttttttga taattgtata atgaaaaaaa ttaatgaatc tataaaaaag gttatttaca
     4081 aaaataaaga agaagataat gttataatta tgataactgc atctccatat gcatatatga
     4141 aatattttaa gtattatggt cttgcagacg aggtcatagg cacggagttt ttttatgaaa
     4201 attcaagata taaaaatcaa ttcattggag aaaactgtaa gggaattgaa aaagtaaaaa
     4261 gaataaaggc tgtattaggt aaacttggga tagagattga ttatgaaaat tcatatgcat
     4321 attcagactc taagagtgat ttaccaatgc tttcattgac taaaaatgca tttttagtta
     4381 gtaaaaagga tggtgaggtt atagagcaaa taaattcata acttaatgtc acttagaata
     4441 ggagggaata gtttgggata tttacacata ttaattttat ctatggttcc tgtaacagaa
     4501 ttgaggggag ccattcctat aggtattgca atgggcttaa atcctatatg ggtttatgta
     4561 tttagtgtaa taggttctac attggtatcg attcccttga tattgacatt tagacatgta
     4621 ttacaatttt taagagggaa gaaactattt aaagggattg ctgatgtaat tgatagaaaa
     4681 ataaactcaa gaatgaagaa attaaaaagt gtttcgataa ttggtataat actatttgta
     4741 ggtataccac tgccaactac aggaacatgg actgcatctg ctatagcttc catattaaaa
     4801 atgagaataa aagatgcttt tatgggagta tttcttggaa atttgttatc aggggttata
     4861 gtttcagctc tttctttaca tataatataa aactatctaa gtaatatact tggatagttt
     4921 ttttatgagt acgtgtgaaa aatttttaat aataaataaa aaatgagata aagtatttaa
     4981 attcagttag ttctaaaatg tttactatgg caaaagaaat atagtatagt attacttgta
     5041 gggaaagtac tctaaatttg tctgctaata aaatgaaatt tttatataga ataaaatttt
     5101 agtgatacat tttaattatt ggattgagga ggttttaaaa tggaaaattc aaacactaac
     5161 ttaggcagtg aaagtgtggg taaattatta tttaaacttg ctacacctgc tattattgca
     5221 caaattgtaa atgttttata caacatagtc gataggattt ttattggtag aatggaaaat
     5281 ggagaagttg ctatggctgg tgttggagtg gcatttccaa taataattat aataactgca
     5341 tgtagttatt taattggtat gggaggaggt ccattagctg ctataaaaat gggtgagcaa
     5401 aacaatgatg aggcagagaa aataatgagt aatagttttt ctgtactggt gattttagca
     5461 atacttttga ctataggttt taagattgga aaagagcctt tactttggat gtttggagca
     5521 agcgaatcta caattggata ttcaatggac tatttaaata tttatttaat aggtacagtg
     5581 tttgttcaga tttcaatggg tatgaatact tttataaata cccaagggtt tgctacaact
     5641 ggaatgatga cagttgctat aggtgcttta ataaacataa tacttgaccc gatttttata
     5701 tttggcttta atatgggtgt aaaaggagct gcattagcta ctataattgc acaaggtgtt
     5761 tctgctattt gggtacttat gtttctcttt ggaaagaaaa gtatacttaa aataaaaaag
     5821 aagtatatga taccaaaagc aagtattatc ttaccagtgc ttggcttggg aatttctcca
     5881 tttataatgc aatctacaga aagtttagta cttatagcct taaatagtaa attacaaatg
     5941 tatggaggag atttagcagt aggttcaatg gccataatga gcagtataat gcaaattctt
     6001 atgcttccta atatgggggt tacacaagga gcacaaccaa taataagtta caattatggt
     6061 tcaggtcaac ttgatagagt aaaaaagaca tttaagttat gtttattgtc atgttttaca
     6121 tattctacta ttttgtggct tttattaatg atatttcctg cattttttgt tagtatattt
     6181 aataagaatc cgcagttact tagtatgact agttggagta taaaaatata ttttgctggt
     6241 gcttttatgt ttgggattca aatagcttgt caacagacat ttttagcact tggaaaagca
     6301 acaatatctt tagttttagc tttacttaga aagattgtac ttttaatacc tttaatattt
     6361 atacttccaa cgttctttaa tgaaaaactt tttgcagtaa ttttagcaga gccagttgcg
     6421 gatataacag ccgctactat aacagcaatt tctttcttta tattttataa atgttttcta
     6481 tcaaaaccta aagcaataaa agaataagat atacaatata aattttttat gaaaatattt
     6541 tattagataa aaattagaat ttgtatttta atttaataag atattttcta taatgtataa
     6601 atatatgaag ttgaatttat tttaaaaatt atagtaagaa aaatgtaagt atttgcaata
     6661 ttaatggtat aatatttaca gatatgataa tcaataagag gaaaggatgt ttataatgga
     6721 aaatgattta acaatgcaag aattacttga ccagcaagaa caagtattca gcaaagtgaa
     6781 agttggagaa ttgacaactg gaaaaataac tgcagtgaga aatgatgaag tacaattagg
     6841 attagactat gggtttgatg gaataatacc tataagtgaa ttgaacatag aaaaaaatca
     6901 atatatagaa gatatatacc atataggtga tgaaataact gctgtaataa caaaagtatc
     6961 tcaaaaagat ggtacaataa ctttatctaa attacaactt gacaaaagaa atgattttgc
     7021 cgagttacaa aaggcttatg atgaacatag aataataact gtaaatgttg aaaagaatat
     7081 agataaaggt gtatttgcta attacaatac atatacattt tttataccaa tttctcaact
     7141 tgatacaaaa tttattacag atacatctaa atttgttggt cttaacttag aagtttatat
     7201 aaaagaattg gatgttagaa aaaatagact tgtagcatct catagagatg tattacaaga
     7261 gcgtataaat aaagagagag aagaaagaag agctcaaatt aaggcagaaa aagaagctga
     7321 aagagcaaga attaagcaag aaagagaaga agaaaaagct cgtatcaaag cagctaaaga
     7381 agacttattt aattctcttg aagttggaca aaaaagagat ggtaaggtga caaagataat
     7441 gccttatggt gcatttgtgg atattggtgg gattgaagga ttagcacata taaataattt
     7501 agcttggaca agagttgaaa gtgttgaaga tgttgtttca gagggacaag aagtagaagt
     7561 ttatgtgttg gatgtaaata aagagactaa aaaaattgcc ttggcattaa aagacataaa
     7621 taatgaccct tgggatttaa tagcaaaaga agtacaaatt gatgatgttg tcaatgctaa
     7681 agttcttaga ataatagaaa aaggggcttt tgttcaaatt aaagaaggtg tagatgcgta
     7741 tttaccaata tctgaattga gtgatactag agtggctaaa gttactaatg tggttaatat
     7801 tggagatgag gttaaagtta agattcttga ttttaaacca aagactaaaa gaatgttggt
     7861 ttcaataaaa gaagcaacta gagagccaga ggaggatata acagaatacc tagaagtaga
     7921 ggaatcttta ggaagtattg gagaattatt taaagataaa tttaaagatt tagaagtgta
     7981 aaataattga aaatggggct gttttaaaac gttacactgt ctattatgac agctttagtt
     8041 ttgaaatagc cctttagcta ttttcacgaa tttatgtaaa atattaggag gaaatttaat
     8101 gaaaaaatct attatggatg tacattgtca cactttaata agtggacatg cacatagtac
     8161 tttcaaggaa aatgtcgaag aggcgagtaa gaagaatata aaatatttag gaatatcaga
     8221 ccacggacct aatatgccag gaggaccaca tccattctat ttttataatt tgcatttgct
     8281 tccacgtgaa gtgcaaggtg taaagattct tagggggata gaaggaaata taatggatta
     8341 tagtggtaat ttagatattc aagaagatat gttgcaacat ttggattata taatcgcaag
     8401 tttgcataga ccttgtatag cttcaggcac aaaagaagaa aatacaaatg ctattttaaa
     8461 agttatggat aaacctaagg taaaaataat tggacatcca gatgacagta ggtatccttt
     8521 agattatgaa cctatagtaa aaaaagctaa agataaaaat atccttttag aaataaataa
     8581 ttcatcatta agttcaaact ctcatagaac gggtacttgg gaaaatgtaa gtcatatgtt
     8641 gacattgtgt aaaacatatg gtgcaagagt aattcttggt actgactctc atatatgtta
     8701 ttctatagga gaatttgaaa atgcagaaaa agttttagaa gcagtagatt ttccagatga
     8761 attagtcata aattatcatg aagatgaaat tatagaattt tttgatataa atttttagaa
     8821 tcttgggagt gaaaaaattt gagtaaaaat aagtttatct atatgaatta ttatgacaat
     8881 ggttatttta aaaaaggaat gactatatat atggttctag cacttataat tttagctaca
     8941 atttttatgt attcaacgac aatattatat attttatgta taataacagc agtttttcta
     9001 tttaaacaag gtcgtgattt ttcaaataaa tatgagggaa aagtgtatat tactctcgat
     9061 aatcattcaa tattaataaa taatcaatgt atattcagta taaaatctaa acaaagtcaa
     9121 aagtttaatt ataagataat agaaaaaata gaagtagtaa aaaatatatt aaatatatac
     9181 actgatgaat ctaattataa aattagatta agggcattga gtttggaaga tgaaaaaaaa
     9241 ttattgaata ttatagatga aaagatgaaa aagtttaagg cataatatca tactgttttc
     9301 aatagttagc atgttttata gaattgctta tcaaaaataa aaaattgaga taattatttt
     9361 ttgtgttgac aatacatttt aatgtgtata taataactat taaatcaaaa tatttagaaa
     9421 atttaaatga ttaaaaaagt gatgaataga agtagtaaga acaatgaaac tttcagagag
     9481 ctaaaggatg gtgtgattta gtagttgata gttctgaact tgtctaggag ctgttttcta
     9541 agatttaaat cttagaataa tcggagtaat tccacgttat aggaaatata gtcattataa
     9601 ttattgtatt ggctattact aagggagctt tttagaaagc taaatagagt ggtaccgcga
     9661 gcaaaacctc gtctctaatt tcattagaga tgaggttatt tttattctaa tgataaaatt
     9721 gacatttatt cttatgaatt gtgcaggatt gcgtcgatat aaggtatact gaatacaaaa
     9781 aacaaaaaag ggggaaataa catgaagtgt ggaaagtata aaaaatatga taaaatgcaa
     9841 atagtaaatc gtaaatggcc agataatgaa atatttaagg ctccaatttg gtgtagtgtc
     9901 gatttaagag acggaaatca gtcactgcca acacctatga gtgtaaatga aaaagtcaga
     9961 atgttcaaaa tgcttattaa tacaggattt aaagaaatag aggtaggatt tccatcagca
    10021 tcaaatacag agtatacatt tttaagaaaa ttaatagatg agaatatgat tccagatgat
    10081 gtaactatac aagtacttac acaatctagg gctcatctta tagaaaaaac atttgaatca
    10141 ataaagggat gtaagaaagc aattatacat ttatataact ctacatctgt tcttcaaaga
    10201 gatgtggttt ttaatatgag taaacaagaa ataatagata tagcagttga aggagcaaaa
    10261 ttatttaatg aagaagtaaa aaaatatcca gaaacagaat ttacttttga atattcacca
    10321 gagagtttta caggaacaga gatggattat gcacttgaaa tatgtgaggc agtgatagct
    10381 gtttggaaac caactcctca aaagaaagta ataataaatc ttccatcaac agtggaaatg
    10441 gcaacaccaa atgtatatgc agaccaaata gaatggtttt gtgaaaatgt aagttgtaga
    10501 gattctatta tactaagttt gcatactcac aatgatagag gaacttgtac ggctgcaagt
    10561 gaacttggct tattggctgg tgccgataga cttgaaggaa cactatttgg aaatggtgaa
    10621 agaactggga atatggatat agtaaatgtg ggacttaatc tttatacaca agggatagac
    10681 ccagagctag attttagcaa catagataag ataagagaaa tatatgaaga ctgtacaaaa
    10741 cttatggtac atgatagaca tccatatgca ggtaatcttg tacattgtgc tttttcaggt
    10801 tcacatcaag atgctattag aaaaggcatg atagcaatga aaaacagaga taatgattac
    10861 tgggaagttc cgtatttacc aatagaccct catgatattg gaagagaata caaagaaatt
    10921 atcaggataa atagccaatc tggaaaagga ggagctgttt atataatgga aacagattat
    10981 ggatttatga tacctaagaa tatgcattct gattttggaa atgtagtcaa aatggaatct
    11041 gatagaattg gggaagaact tagtagtgaa gcaatattca atttatttaa gaaagaatat
    11101 attgaagttg agagccctta taaagttaag aagtataaaa taaaatcaat ggatgaatta
    11161 aactatgaaa atgatgattc taatgatact aacatgattg agatgactgc aagaataagt
    11221 tatatgggaa atgaacaaag gattgttggt ataggaaatg gacctgtaga ttcatttaat
    11281 aatgcactaa agcaatgtgg catgaaagat tataaattta gatattactg ggaacatgca
    11341 ttagaggaag ggtcacattc aagaggggta gcttatgtag gaatagagca caataatgaa
    11401 gtctattttg gtgtgagtat atcggaaaac ataaatacag cagctattaa tgcacttatg
    11461 aatgcaataa ataaaagtta tattgaggag gaaataaaaa atggggatga ctatgacgca
    11521 gaaaatatta gccaaacatg ctaaattaaa tgaggttaag aaagggcaat taatcgaagc
    11581 agatttagat gtagttttag gtaatgatat aacatcacca gtagccatta gagaatttga
    11641 aaaattagga attgaagatg tttatgataa gactaaagta gtgatggttt tagaccattt
    11701 tacacctaat aaagatataa agagtgcaga acaatgtaaa tttacaagaa gtttcgcaaa
    11761 atcaaaagga gttgtaaatt tttttgatgt aggagatatg ggaatagagc atgttcttct
    11821 tcctgaaaaa ggtatagtaa ctgctggaga tgtaattata ggtgctgatt ctcatacttg
    11881 cacatatgga gcattaggag cattttctac tggtgtaggt tcaactgata tgggagcagg
    11941 catggctaca ggaaaatgtt ggtttaaggt tcctggagca ataaaatttg ttttgaagaa
    12001 taaaccaaat aaatggataa gtggtaaaga tataatactt catataatag gtgagatagg
    12061 tgttgatgga gcactttata agtcgatgga attttgcggg gatggagtag aatacttatc
    12121 tatggatgat agatttacta tttgcaatat ggcaatagaa gcaggagcaa aaaatggaat
    12181 ttttccagta gacgataaga ctatggaata tataaattca cataagtgtt caactataac
    12241 aaaagatgta aatatatatg aagcagatga agatgcagtc tatgatgaag tatacgaaat
    12301 agatttagct gaactaaaag aaacagtagc ctttcctcat cttcctgaaa atactagaac
    12361 agtagatgat atagataagg atataaagat agaccaagtt gtaataggtt cttgtacaaa
    12421 tggaagaata tcagaccttg aagtagtagc tgagataatg aaaggcaaaa aagtagcaga
    12481 tggagttagg gttatgatat ttcctggaac tcaaaaagta tatcttgaag ctatagaaaa
    12541 agggtatatt accactttta tagaggcagg agcagcagtt tctacaccaa cttgtggtcc
    12601 atgtctaggg ggacatatgg gtatacttgc agcaggagaa aaatccattt caacaacaaa
    12661 taggaatttc gtaggaagaa tggggcatgt tgattcagag gtgtatcttg caagcccagc
    12721 tgtagcagca gcatcagcta taactggaaa aatatcaaaa ccatcagaaa taatttaagg
    12781 gaggtaaaat ttatgattgc caatggaagt gtttttaaat tcggtgataa tatagatacg
    12841 gatgtaataa taccagctag atatttaaat atagctgatt ataaagaact agcaacgcat
    12901 tgtatggagg atattgatga taactttatc tctaaggtaa aagaaggaga cattatagtt
    12961 gcaactaaaa actttggttg tggttcatca agagaacatg caccaattgt aataaaagaa
    13021 agtggagtat cctgtgtgat tgcttctact tttgctagaa tattttttag aaactctata
    13081 aatatagggc ttcctatact tgaatgcgaa gaagctgcca ataacataga tgaaggggat
    13141 aatatagaag tagatttttc tacaggtgtg ataaaaaata taacaaaagg aaaagaatat
    13201 aaagcagaac catttccaga gtttatgcaa aatattatat taaatgaagg cttaattaac
    13261 tcaataaaag caaatagggg gtagtggagt atgaattgta atatagcagt cataaaaggt
    13321 gatggtgttg gaccggaaat tatagatgaa ggtataaagg ttctaaataa aatttgttgt
    13381 aaatttaatc atagattcga ttgtgaatat gtattagctg gaggatgtgc aattgatgaa
    13441 actggagaac ctctacctaa taaaactgtt gaaatttgtc gaaaaaacga agctgtatta
    13501 cttggtgctg ttggaggagc aaaatgggat aaatgtaagg gagataaaag accagagtct
    13561 ggtcttttaa aattaagaga gtcattgggc ttatttgcaa acttaagacc agctacaatg
    13621 tatgaaagta taaaagaagc ttcaccactt agaacggata tagttgagaa aggcattgat
    13681 tttgttgttg taagagaatt aacagggggc atttattttg gagaacgtgg aagaaaaatc
    13741 attgatggta ttgaaaatgc ttatgatgtg gaaatatata atgaaaatga aataagaaga
    13801 attggtaaga gagcttttga aatagccaga aatagaaata aaaaacttat aagtgtagat
    13861 aaggcaaatg tgcttgagag ttcaagactt tggagaagta taatggaaga cttagctaaa
    13921 gagtttgaag atgtagaact gtcacatatg tatgttgata acgctgctat gcaagttgta
    13981 aaagacccta gccaatttga tgtaatagta actaataaca tatttggaga tataatatct
    14041 gatgaagcat ctatgattac aggttctata ggtatgttac catcagcatc acttagagaa
    14101 gatagttttg gtatgtatga accaatacat ggaagtgcgc ctgatatagc tggaaaagat
    14161 gttgtaaatc caattgcaac tattctatct gtaagcatga tgttaagaca tagttttaac
    14221 ttagaagaag aggctaagtg tattgaagat gctgtacaaa gtgtactcaa taaaggttat
    14281 agaactattg atatctataa tggtgtggga aatgttgtag gaacaagaga aatgggcaaa
    14341 ttaatagtaa atgaaattta gaattatagt ttaatttgaa taatataatt taaatatata
    14401 acttgtaaaa ttagtacaaa tctattgtaa tataatttgt tttatgaggg gtgtctcaaa
    14461 ataaacttta ttagttaatg agacaccctt ttttatattg agtcaaatat atttttacta
    14521 ttttaataac gaaaaagagg tctatctcta ctttaagttt ctattttgat aaagttattt
    14581 ttttagaaaa taatatttta aattaaactt atcatattga agtagtctat ttttattatt
    14641 agaagtttgc agggatttta ttaatattat ataatatatt ttagaattat ttaatattaa
    14701 gcgactacta gtgtcggtta ggagatggat tttttggcaa agtataaagt tttgaagaag
    14761 caaaatagta gtaaatcttg tttagtttgt ggaacacaaa atgaattagg actcaaagca
    14821 gatttttatg aacttgaaaa tggagagctt gtatcaattt gtaatacaaa ggattggcat
    14881 caaagttatc ctgggagagt tcatggaggt atgtctgctg caatattgga tgagactatt
    14941 ggaagagctg taagtataaa tgatgaccaa atatggggag taacagtatc tttagaattg
    15001 aaatataaga aaccagtacc aacagatgct actataaaag ttgttggaag aataactaaa
    15061 gaaaacagaa aattatttga aggtacagga gaaataattc taccaaatgg agatatagca
    15121 gtgacagcaa ctggaaagta tatgaaaatg cctattggac aaattgcaga aggtgatttt
    15181 tctaatgaag aatggttttt tgaagaatcg aaagaaaaag tggaatatat tgaactttga
    15241 ttaaataaaa att
//
LOCUS       DAEPXF010000089        15064 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain HB27
            SAMN13376389-rid12479423.denovo.094, whole genome shotgun sequence.
ACCESSION   DAEPXF010000089 DAEPXF010000000
VERSION     DAEPXF010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13376389
            Sequence Read Archive: SRR10518432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 15064)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15064)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001069624.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001069624.1
            Genome Coverage       :: 315x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/15/2021 01:54:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,165
            CDSs (total)                      :: 4,076
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 89
            rRNAs                             :: 10, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 10 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 19 of 69
            Pseudo Genes (incomplete)         :: 44 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 10 of 69
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15064
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13376389-rid12479423.denovo.094"
                     /strain="HB27"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="China: Hebei"
                     /collection_date="2017"
     gene            complement(<1..970)
                     /locus_tag="KRL67_002352"
     CDS             complement(<1..970)
                     /locus_tag="KRL67_002352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane dipeptidase"
                     /protein_id="HBH1523226.1"
                     /translation="MIFDGHSDTWADVTIRRLNGEKDVFRRIHLDNFKRANIEGAIFV
                     IWIDPPYDKEPEKRVKQIISSIKDEIEDMKDVVNIVKKYSDIDKGISENKINILIGLE
                     GLSHIGRNIDLLDYFYKEVGARHASLTWNEQNELATGCLGDENRGLTEIGKKAVNKME
                     KLGMVVDVSHLNEKSFWDLMSIASKPVIASHSNARSLCDAKRNLTDNQLKEISNTDGL
                     VGINSFRGFTNSNPKKQDLIHLVDHIDYMVNLIGIEHVSFGFDFSDYLGKETIGSFTG
                     NASMPSVDGLESTHTAHNIVVELENRGYKKKDIDLISSGNFYRLFRD"
     gene            complement(1026..2228)
                     /locus_tag="KRL67_002353"
     CDS             complement(1026..2228)
                     /locus_tag="KRL67_002353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF819 domain-containing protein"
                     /protein_id="HBH1523227.1"
                     /translation="MKSFVSPDNIWVLWAVVTGWAAFSIYLEQKYNWASKVSGAIIAL
                     VGAMLLSNLNIIPVESVVYDQVWGYVVPLAIALLLYQCNIKKIWKESGRLLIIFLVGS
                     VGTVLGAMIGFLALKNVVPDLNIVAAMMTGSYIGGNVNFAAMSGAFDAPGELVSATVV
                     ADNLLMALYFFVLIAIPSIGFFRKHFKHPHVDEMESIGITEGETVAANYWGRKEISLK
                     DIAIAIGSAFIIVAVSVELSTWFKAIIPLSNPFLAMLNTLFGNQYLIITTLTMLFATF
                     MPNFFGDIKGAQELGTFLIYIFFVVVGVPASITLIIQKSPLLLLYCGIMVLINMIVTF
                     IVAKIFKFSLEEAILASNANIGGPTTAAAMAISKGWSKLVGPILIVGTFGYIVGNYFG
                     LLVGNILI"
     gene            2423..2977
                     /locus_tag="KRL67_002354"
     CDS             2423..2977
                     /locus_tag="KRL67_002354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HBH1523228.1"
                     /translation="MNIDVGSKIRSLRKSKNISISTLAKNSDLSTGLISQIERNMVVP
                     SIVAMWKISKALDINIGYFFEEIGREDSDIVVKKNNRKKIVTNDSTKFYELLVPNLSG
                     KKIEFILVTLDGHTQKNSEFVTHEGEECGYIIKGKMKITLGNKEYLLEEGDSFYFNST
                     IPHVYENYDDEVCISVWAMTPPSF"
     gene            complement(3092..3883)
                     /locus_tag="KRL67_002355"
     CDS             complement(3092..3883)
                     /locus_tag="KRL67_002355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase family protein"
                     /protein_id="HBH1523229.1"
                     /translation="MIVFSDLDRSIIYSNKFLNADSKYANIEVYREKEISYISLDTIN
                     LIKQIQYYGMFIPTTTRTVEQFKRIEFNKYGIYFPWSITSNGGVILKDNEILKSWSEK
                     IDKLKSDYEPIESMIYKFKDYLNVDGITNFKVAEDIFFYIVVDFSKFNLESIKEYTNV
                     LESKNWKFYVSGRKIYFIPKEISKENAIKYLTKELGIEYFYAVGDSIMDYGMLNISNK
                     SYVLKHGDINKNEIENSFISSSFNGMNGTEEILSNILDESCLLNI"
     gene            complement(3911..4999)
                     /locus_tag="KRL67_002356"
     CDS             complement(3911..4999)
                     /locus_tag="KRL67_002356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine protease StiP family protein"
                     /protein_id="HBH1523230.1"
                     /translation="MRDFSTYSKDDVIFLLKDISNLIEEEGNKERELKIQNGRHYSEM
                     IPIEYEVSEDYLNLYHEKLRENKDKLAFSIGVMCEKIIKKNGNDVVLVSLARAGTPIG
                     ILAKRYVKSKYNINLPHYTISIIRDKGIDMNAIEYIVKNHPSSKIQFLDGWTGKGTIS
                     KELEKACDELNVKFKKHFDSTLAVLADPAGYSGLYGIREDFLIPSACLNSTVSGLVSR
                     TVLREDLIGKNDFHGAKFYRHLKDKDESINYIETIEECFKNQFKNISDEVENWESDII
                     TRDGYFDVLNIKEKYNITDINFIKPGVGETTRVLLRRVPYKILVKDLNDKSLDHIFVL
                     AKEKNVEVEQMDLKAYKCCGIIKNMKDV"
     gene            complement(5053..6285)
                     /locus_tag="KRL67_002357"
     CDS             complement(5053..6285)
                     /locus_tag="KRL67_002357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HBH1523231.1"
                     /translation="MKNCLNFNCEIDIIDNPLNLELNDFLDVGIRNNSKRRFLFISKK
                     LGKHLACKPSEMDNLGKLMATIYNEKNKEESNGTVIAFAETGTAVGHSFFDYLCGDYE
                     FIHTTREQFDELENLEFLEEHSHATDQNLYFGMLNNFKFGDEIILVDDEITTANTCMN
                     IIRKIQSIYPKKKYTICSILNWISDENLSKVTRLEKELDCKIDFVYLFKGNFKFYIDE
                     DIISNYNDNYSITKLETISSDKVNLVVNHIYVDLEDYIGNKKYVKYTGRFGINRREQF
                     RLKEIVKRESKKITMDSKESLKKEKILFLGTEEFMYIPMLFAKEKENQCDIYYHSTTR
                     SPIINIDKNNYPIKSKFMLKSFYNIDIQNYIYNIDKHNFSKCYLFVELNKSENSYLEF
                     IDIIKKTSIKELTIVCCS"
     gene            complement(6263..7339)
                     /locus_tag="KRL67_002358"
     CDS             complement(6263..7339)
                     /locus_tag="KRL67_002358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HpcH/HpaI aldolase/citrate lyase family protein"
                     /protein_id="HBH1523232.1"
                     /translation="MKYFDYICKDDLERIFLKEPEDFSAKTEKDVLKYALGAFLYVPA
                     TQYNMIYKSIIGDVKGVRPLAICLEDAVGVNGELEAIENLRLILKNISNESITNKDGI
                     PLIFVRIKDVEQLLRIKEIIIKNSHSITGILIPKANSELIENCIEALDSMNLQDMYVI
                     PIIETKEFIYNEKKELSFTNLYNAILRHKSRILSIRVGITDILGMYGIRRDKNFSIYN
                     NLICSSFILDIITYLQRPELDIPISGGVSEFFDMTNKDIRNKYIEEILLDKYHGLIGK
                     TVIHPMQIQIVQALSTVSYEDFTDALDILDSTDSKYGVSKGVLGERMNETNPHFLWAK
                     KTLILSKIYGVLNKGVDYEELLKF"
     gene            complement(7403..8539)
                     /locus_tag="KRL67_002359"
     CDS             complement(7403..8539)
                     /locus_tag="KRL67_002359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic anion resistance protein"
                     /protein_id="HBH1523233.1"
                     /translation="MGININSFSKDKNSVEVTENLPTAVQEENFDIMEYTNNKKNELR
                     KSKEVEALTSLIEVENPDTILQFGRKASEGVARVSDSLLNTIKLNRNEENSKMLVHLT
                     KIMDKFDLDDFQETKEPNFVQKLFKKANNAIEMMFQKYETLGGEVEKIQIELEQYERD
                     IALSNKQIGAMLNENFEFYNELQKYIVAGEMAIEEMDNEILPFFKQKSETSGDQMDVV
                     NYQELLKVYDMLNQRVYDLRIAENIAIQTIPMLRGMQHNNYGLIRKINSAFVVTLPVF
                     KQCLSQAILLKKQELQAKSLKALDDKTNELLLRNAQNVSTQSAQIARMAGTSSVQIET
                     LEKMYNTIKSGIDETMRIEENNRALIKDNTKRLEELNTTIIYNK"
     gene            complement(8540..10102)
                     /locus_tag="KRL67_002360"
     CDS             complement(8540..10102)
                     /locus_tag="KRL67_002360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceG family protein"
                     /protein_id="HBH1523234.1"
                     /translation="MGDSKVFEKIFSSQSDRGNYTPSKGYLSYFISYIGLEDEVLYNL
                     EIFKAKQNIDSKKDIALFTDVIANPSDFDIINYFKSGLQKYRTSMEDVDINILGFEEI
                     DYKIKQAMDRVLKEEEKEFTNDRVKQNFIVKIMAWIKIYIGALDINKNEAPKVIFYGD
                     IKKHEVYLLLILYLAGFDVLYLNPNSKSNIDILKSERYNIEFEEANIIEEKVSFEERV
                     ILGEKIDKSSVKKAFTVGAEASKRISEELLNDAGFIKPWQLQDRKIKNLLLSSTVDEI
                     SIYWNQPLKLRPGFKFNDAIVEAPNFLSKINGIYNDKNEYIKFLDLLRDSESSTFIEF
                     NGDVDRFSKAFTREAFSLSFLLDSKGVIDKNSVLNNKDYSISTLALNQQIMILEKVEE
                     LLEGSMFLNGLSGEDRIKGLFTVLHMDKKFVHMMNNFDYSLINPKLIIYMYKSIVFDK
                     EIVFLMLLLSKIGFDIIILCPGGENNIENVINNQLIDVHRLDKMVYDLKLNSLENDIP
                     LLKKIFGKRRRF"
     gene            complement(10189..12948)
                     /locus_tag="KRL67_002361"
     CDS             complement(10189..12948)
                     /locus_tag="KRL67_002361"
                     /EC_number="7.2.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-translocating P-type ATPase, PMCA-type"
                     /protein_id="HBH1523235.1"
                     /translation="MEFNNDIKYYNVDKEEVTKDLSVNPEKGLSESEVKTRREKYGLN
                     EFTPKEEGSFWDDLKESLSEPMIVILIIAAVVSAVIGETHDAIGIVGAIAIGIAIGMI
                     TEGRSKKAAEALSKLTENIEVKVLRDGEVHQISKSELVPGDIVYIETGDMVPADGRLI
                     ESINLKIREDMLTGESDDVSKKCDVVVSMEEIESKGAVVVQEPIPAKQINMVFGGTLV
                     AYGRGALVVTSIGDNSEMGKIAQNLSETDEQTPLQIKLGNLGGLIAKVSSAVAGLLFI
                     FMVFQMVSKNVLNVDMSGILPFLESIDPVKTAFTVCVALIVAAVPEGLPTMINMTLAI
                     TMQKMAKINALVTKKEACETIGSVSVICSDKTGTLTENRMTVEVAYVDGRYIESSEEE
                     INSYFEENCMINSTADVEHNDGDIKYLGSATECALLLYYKNVDYRQVRKNSNIVAQNP
                     FTSDSKRMTSVIGQDNHHVLLSKGAPEVLLELCSHIQRGKDIVPLTENIKHEILEEIK
                     KLQIKSMRTLGFAYKEISQAEEEAAVTAESDAMNVSAMENNLVFSGFVGIRDPLRKDV
                     IESVNTANKAGVSVKMLTGDNINTARAIGEELGLLKNNMRVVEASYIDTLDDEELKQE
                     IQSISIVARSKPDSKMRIVSALQKSGDVVAVTGDGINDAPALSKADVGIAMGISGTEV
                     SKNAADIILTDDSFSTIVKGIKWGRGIYDNFQRFVQFQLTVNVIAFLVAIISQVMGQE
                     MPFTTIQLLWVNIIMDGPPALALGLEPVRDHVLNRKPVDRKANIISKSMVYTIVLNAF
                     YITAILMLQSTFNFLGVDEAHKGTVMFALFAFSALFNAFNCREFNADSIFPNFTHNKL
                     ALQIIVLTGIAQVIFTQLFQDFFNSVAMDFMTWLKVLCVAASVIVVNEVVKCIIRLVK
                     PKKTV"
     gene            complement(12938..13567)
                     /locus_tag="KRL67_002362"
     CDS             complement(12938..13567)
                     /locus_tag="KRL67_002362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerD family protein"
                     /protein_id="HBH1523236.1"
                     /translation="MSINLSKGDKIDLKKSNPGLSNILVGLGWDPVQQSGGGFFKSLF
                     GGGQADIDCDASVFMLNQEGKLSGIKDLIYFGNLKSACKSVLHTGDNLTGEGTGDDEQ
                     ILVNLDKVPSNIHKLLFVVNIYNCVDRKQHFGMIENAYIRVEDQGNKKEIAKYNLSDN
                     YSEKTTLIVGAIYRKDGSWQFKAIGEGTKDAGLKEVMQNLDRIECAYGI"
     gene            complement(13653..14231)
                     /locus_tag="KRL67_002363"
     CDS             complement(13653..14231)
                     /locus_tag="KRL67_002363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_126936537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerD family protein"
                     /protein_id="HBH1523237.1"
                     /translation="MAIVLKKGQKIDLTKGNPGLKNIKLGLGWDTNSFDSGYDYDLDV
                     SVFMVGESQRVEKDEDFIFYNNLKHPSGAVEHLGDNRTGEGDGDDEEILVNLEAMPKH
                     IQKIAVTVTIYEAAERRQNFGQVSNSYIRVLNSENDEEILRYDLGEEFSIETAITVCE
                     IYKYNGEWKFSAVGAGFEGGLEALCRNYGLNV"
     gene            complement(14270..14848)
                     /locus_tag="KRL67_002364"
     CDS             complement(14270..14848)
                     /locus_tag="KRL67_002364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_126936537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerD family protein"
                     /protein_id="HBH1523238.1"
                     /translation="MGITLSKGQKVDLTKGNPGLKNILVGLGWDTNKYDGGFDFDLDT
                     AAFLTGASGSVTNDGDFVFYNNLKHTSGAVEHLGDNRTGEGDGDDEQIVVDLSKIPGE
                     ISKISFTVTIHDATERRQNFGQVSNSYIRIVDKDTNEELIKYELGEDFSIETAIVVAE
                     IYKHNGEWKFNALGSGFEGGLAALCGNFGINL"
BASE COUNT         4834 a         2746 c         1566 g         5918 t
ORIGIN      
        1 tatctctaaa gagcctataa aaattaccac tggagattaa atctatatct ttctttttat
       61 aacctctatt ttcaagctct actactatgt tatgtgctgt atgtgtgctt tcaagaccat
      121 ctacacttgg catactagcg tttccagtaa aagaacctat agtctctttc cctaagtaat
      181 cactaaaatc aaaaccaaaa gatacatgtt ctatacctat aagatttacc atatagtcta
      241 tatggtcaac caaatgtatt aaatcttgct tcttaggatt actattagta aatcctctaa
      301 aactatttat tccaactaat ccatctgtat ttgaaatttc ttttaattga ttatctgtaa
      361 gatttctttt agcatcacat aaactcctag cattagaatg agaagctata actggcttac
      421 ttgcaataga cattaaatcc caaaatgact tttcatttaa atgtgataca tctactacca
      481 tgcccaattt ttccatttta ttgactgctt ttttacctat ttcagttaaa ccacgatttt
      541 catcccctaa acatcctgta gcaagttcat tctgttcatt ccaagttaat gatgcatgtc
      601 ttgcaccaac ttctttataa aaataatcta gtaaatctat atttcttcca atatggctta
      661 atccttcaag accaattaaa atatttattt tgttttcact tataccttta tctatatctg
      721 aatatttttt tactatattt acaacatctt tcatatcttc aatttcatct tttatagagc
      781 ttataatttg tttaactctt ttttcaggct ctttatcata tggtgggtct atccatatta
      841 caaaaatagc tccttctata tttgctcttt taaaattatc aagatggatt cttctaaata
      901 catctttttc cccgttaagc cttcttattg ttacatctgc ccaagtatct gaatgtccat
      961 cgaaaatcat aacaacctcc aattaaataa aaatctttct aaaaacaact ttttttcacg
     1021 taaaattata tcaaaatatt tcctactaaa agaccaaaat aattaccaac tatgtatcca
     1081 aatgttccaa ctataagtat tggccctacc agcttagacc atccttttga tattgccata
     1141 gccgctgctg ttgtaggtcc accaatattt gcattagatg caagaatagc ttcttctaaa
     1201 ctaaacttaa atatttttgc aactataaat gtaactatca tgtttattag taccattatt
     1261 ccgcagtata aaagtagtaa tggtgatttt tgaattatta atgttattga agcaggaaca
     1321 cccactacaa caaagaatat gtatattaaa aatgtaccca actcctgtgc tccttttata
     1381 tcaccaaaga aattaggcat aaatgtggca aacaacatag tcaaagtagt tattattaga
     1441 tattgatttc caaataaagt atttaacatt gctaaaaaag gatttgataa tggtattata
     1501 gctttaaacc aagttgatag ttctacagat actgcaacaa ttataaaggc gctaccaatg
     1561 gctattgcta tatcttttaa agatatttct tttcttcccc aatagtttgc tgctactgtt
     1621 tctccttcag ttatacctat actttccatt tcatctacat gtggatgttt aaaatgtttt
     1681 ctaaaaaatc caattgatgg tatggctatt aagacaaaga aatataatgc cattaaaaga
     1741 ttatctgcta caactgttgc tgatactaat tctcctggcg catcaaatgc ccctgacata
     1801 gcagcaaagt ttacatttcc acctatataa gaacctgtca tcatagctgc aacaatattt
     1861 aaatctggta ctacattttt taatgctaaa aatccaatca tagctccaag tacggttcct
     1921 acagaaccta caagaaatat aatgagcaat cttccacttt ctttccatat ctttttaatg
     1981 ttacattgat ataacaataa agctatggct aaaggaacaa catatcccca tacttggtca
     2041 tatacaacag actctactgg tataatattt aaatttgata ataacattgc tcctacaaga
     2101 gctattattg ctcccgacac tttcgatgcc caattgtact tttgttccaa gtaaatacta
     2161 aatgctgccc atccagttac aactgcccat aatacccaaa tattatcagg gcttacaaaa
     2221 ctcttcatct taattacctc catttcatta aattaaaata tcgttatcaa tattcaatat
     2281 attgaatatt aatactatta taatgcatct tttcagaaaa ttcaatataa cataaaaaat
     2341 acatgcatta taaaactttt ttttatttta ttaatcttta taaattatat gttataatca
     2401 tgtctgaggg ggtttgtatc taatgaatat agatgtcgga agcaaaatta gatctcttag
     2461 aaaatctaaa aatataagta tctctacttt agcaaaaaat tcagatttaa gtacaggctt
     2521 aataagtcaa attgagcgta atatggttgt tccatctatt gtagcaatgt ggaaaatatc
     2581 aaaagctctt gatataaata taggttattt ttttgaagag attggaagag aggactctga
     2641 tatagtagta aagaaaaata atcgaaaaaa gatagtaact aatgattcta caaaatttta
     2701 cgaacttctt gttcctaatt tatctggaaa aaaaatagaa tttatacttg ttacactaga
     2761 tggtcataca caaaaaaaca gtgaatttgt gacacacgaa ggagaagaat gtggctatat
     2821 aataaaaggt aaaatgaaga taacacttgg aaataaggaa tatctattgg aggaaggaga
     2881 tagtttttat tttaacagta ctatcccaca tgtgtatgaa aattatgatg atgaggtatg
     2941 tatctcagtc tgggcaatga ctccaccttc attttaacat gttgagttct tacttaaata
     3001 taaattgata aaaatctgaa ataaattagt atgataaact ttactataat ttatttcaga
     3061 tttttctagt ttattattta tatatcagtc attatatatt aagtaggcaa ctctcgtcta
     3121 aaatgttaga tagtatctct tcagttccat tcattccatt gaaagaactt gatataaatg
     3181 aattttctat ttcattttta tttatatcac catgcttcaa tacataggat ttatttgaga
     3241 tattaagcat accatagtcc ataatagaat ctccaactgc ataaaaatat tctattccta
     3301 attctttagt caagtatttt atagcatttt cttttgaaat ttcttttggt ataaaatata
     3361 tttttctgcc acttacataa aacttccaat tttttgattc caatacattt gtatattctt
     3421 taattgattc taaattaaat tttgagaaat caacaactat ataaaaaaat atatcttctg
     3481 caactttaaa attagttatt ccatctacat tcaaatagtc tttaaattta taaatcatac
     3541 tttcaatggg ctcatagtca gattttagtt tatctatttt ttcactccat gatttcaata
     3601 tttcattatc tttaagtata actcctccat ttgaggttat actccaagga aaataaatcc
     3661 catatttatt aaactctata cgcttaaatt gctccactgt tctagtagtt gtaggtataa
     3721 acataccata atattgtatt tgctttatta aatttatagt atctaaagat atataagata
     3781 tttctttttc cctataaact tctatattag catatttact atcagcattt aaaaatttat
     3841 tggagtaaat gatactcctg tctaaatctg aaaaaacaat cattcaatat tctcctgtct
     3901 aatatatttc ctaaacatct ttcatatttt ttattattcc acaacattta taggctttta
     3961 aatccatttg ttcaacttca acatttttct ccttagctaa tacgaatata tggtctaaag
     4021 atttatcatt taagtcttta acaagaatct tatacggaac tcttctaagt aaaacccttg
     4081 tcgtttctcc tacacctggt ttgataaagt taatatctgt tatgttatac ttttctttta
     4141 tatttaaaac atcaaagtat ccatctcttg ttataatatc actttcccag ttttcaactt
     4201 catcagatat gttcttaaat tgatttttaa aacattcttc tatagtttca atatagttaa
     4261 tagactcatc tttatctttt aaatgtctat aaaacttagc tccatggaag tcatttttac
     4321 caattaaatc ctctcttagt acagttctgc ttactagacc agaaacggtt gaatttaaac
     4381 atgcacttgg tattaagaaa tcttctctaa ttccataaag tcctgaatat ccagcagggt
     4441 cagctaatac agcaagtgtt gagtcaaaat gttttttaaa tttaacatta agctcatcac
     4501 atgctttttc aagttctttt gatatagttc ctttaccagt ccatccatct aaaaattgaa
     4561 tctttgagct cggatgattc tttactatat attcaattgc attcatatct attcccttat
     4621 ctcttattat agagattgta tagtgaggta aatttatatt atacttgctt tttacatatc
     4681 tttttgccaa aataccaata ggtgttcctg ctctagcaag tgaaactagt actacatcat
     4741 ttccattttt tttaattatt ttttcacaca taacgcctat agaaaatgca agcttatctt
     4801 tattttctct tagtttctca tgatataaat tcagataatc ttcactaact tcatactcta
     4861 ttggtatcat ttctgaataa tgtctaccat tttgtatttt taattctctt tctttatttc
     4921 cttcttcttc aattaaattc gatatatctt ttaataaaaa aatgacatca tctttagaat
     4981 acgtgctaaa atctctcata tgactcaact cctatcttaa aatcgcgata tattttatcc
     5041 tgttaaaaca ctttagctac aacatactat agttaattct tttatacttg tctttttaat
     5101 tatatcaata aattctagat aagaattttc acttttattt agctctacaa ataaataaca
     5161 cttagaaaaa ttatgtttat ctatattgta aatataattt tgaatatcta tattataaaa
     5221 actctttagc ataaatttac ttttaatagg ataattattc ttatcaatat taataattgg
     5281 acttcttgtt gttgaatgat agtatatatc acattgattt tctttttctt ttgcaaataa
     5341 cataggtata tacataaatt cttcagtacc taaaaataga atcttttctt tttttaaaga
     5401 ttctttactg tccatagtta tttttttact ttctctctta acgatttctt ttaatctaaa
     5461 ttgttctctt ctattaattc caaatcttcc tgtatatttt acatatttct tatttccaat
     5521 ataatcttct aaatcaacat aaatatgatt tactactaaa tttactttat cacttgaaat
     5581 agtctctaat ttagtaatac tataattgtc gttgtaattt gaaataatat cttcatctat
     5641 ataaaattta aagtttcctt taaataagta tacaaaatct atcttacaat ctagctcttt
     5701 ttctaaacga gtaactttac ttaaattttc atcacttatc caattgagta ttgagcatat
     5761 agtgtatttc tttttcggat aaatgctttg aatcttccta attatattca tgcatgtatt
     5821 ggctgtggta atctcatcat caaccaatat tatctcatct ccaaatttaa agttgttaag
     5881 catcccaaag tataaattct ggtctgttgc atgggaatgt tcctctaaaa attccagatt
     5941 ttctaactcg tcaaactgct ctctagtagt atgaataaat tcataatctc cacataagta
     6001 atcaaaaaaa ctatgcccaa cagctgtacc agtttcagca aaagctataa cagtaccatt
     6061 tgattcttcc ttattttttt cattataaat tgtagccatt aattttccca aattatccat
     6121 ctcacttggc ttacatgcta aatgtttacc gagtttcttt gagataaata aaaaacgtct
     6181 tttgctatta tttcttattc ctacatctaa aaaatcattt agttctaaat tcaatggatt
     6241 atcaataatg tcaatctcac aattaaaatt taagcaattc ttcatagtct actcctttat
     6301 ttaatacacc atatatttta gatagaatca atgttttttt agcccataaa aaatgtggat
     6361 ttgtttcatt cattctctct cctaatacac ccttgctcac accatactta ctgtctgtac
     6421 tatccagtat atccaaagca tcagtaaaat cttcatatga tactgtagat agcgcttgta
     6481 caatttgaat ttgcattgga tgaattacgg tctttccaat taatccatga tatttatcca
     6541 ataatatttc ttctatatac ttatttctta tatccttatt tgtcatatca aaaaattctg
     6601 atactccacc acttataggt atatctaact ctggtctttg aagatatgtg attatatcta
     6661 atataaatga agaacaaatc aaattattat atatagaaaa gttcttatct ctccttatac
     6721 catacatccc taatatatca gttatgccta ctctaatgct taatattctt gacttatgtc
     6781 ttaaaatagc attatacaaa ttagtaaaac taagttcttt tttttcatta tatataaatt
     6841 cttttgtctc tattatcgga ataacataca tatcttggag attcatagaa tctaaagctt
     6901 ctatacaatt ttcaattaac tccgaatttg ctttaggaat taatattcct gttatagaat
     6961 gactattttt aattataatc tctttaattc tcaacagttg ctccacatct tttattctta
     7021 caaaaattag tggtattcca tctttattag ttatactttc attgctaata ttttttaaaa
     7081 ttagtctaag attttctata gcctctaact ctccatttac tccaacagca tcttctaaac
     7141 aaatagctaa tggtctaact cccttaacat cccctataat actcttataa atcatattat
     7201 attgagtagc tggaacgtat aaaaaagcgc ccagtgcata tttcaacaca tctttttcag
     7261 tcttagcact aaagtcttct ggctctttca aaaaaattct ctccaaatca tctttgcaga
     7321 tataatcgaa atatttcaat gtctagcctc ctcatataca tagaaagggc caactttata
     7381 caaattagcc cttaatataa tactacttat tataaattat agtcgtattt aattcttcca
     7441 atctctttgt attatcctta attaaagctc tattgttttc ttctattctc atagtttcat
     7501 ctataccaga cttgatagta ttgtacattt tttccaatgt ttctatttga acagaacttg
     7561 ttccagccat tctagctatt tgtgcacttt gagtacttac attttgtgca tttctaagca
     7621 ataattcatt agttttgtca tcaagtgctt ttagagattt tgcttgtaat tcttgctttt
     7681 taagtaatat agcttgagat aaacattgtt taaatactgg taaagttacg acaaatgcac
     7741 tatttatttt tctaattaaa ccataattat tatgttgcat acctctaagc ataggtattg
     7801 tttggatagc tatattttct gctatcctta aatcatagac tctttgattt aacatatcat
     7861 aaacttttaa tagttcttga taattgacaa catccatttg gtctccacta gtctctgatt
     7921 tttgtttaaa gaaaggtagt atttcattat ccatttcttc tattgccatt tctccagcaa
     7981 caatatattt ttgaagttca ttataaaact caaagttttc atttaacata gcacctattt
     8041 gcttattaga aagtgctata tctctttcgt attgctcaag ctcaatttga attttctcaa
     8101 cttctcctcc taatgtttca tacttttgaa acatcatttc aatggcatta ttagcttttt
     8161 tgaaaagttt ttgaacgaaa tttggttcct tagtttcttg aaaatcgtct aaatcaaact
     8221 tatccattat tttagttaaa tgaaccagca ttttagaatt ttcttcattt ctatttaatt
     8281 ttattgtatt taaaaggcta tcagaaactc ttgctactcc ttcagatgct ttcctaccaa
     8341 actgtaaaat cgtatctgga ttttctactt ctataagaga tgtcaatgcc tcaacttcct
     8401 tagactttct cagttcattt tttttattat ttgtatattc cattatatcg aagttttctt
     8461 cttgaactgc tgtaggcaaa ttctcagtaa cttctacaga gttcttatct tttgaaaaac
     8521 tatttatatt tatccccatc taaaatcgtc tcctctttcc aaaaattttt ttaagtaaag
     8581 gtatatcatt ttcaagcgaa tttaatttaa ggtcatatac cattttatcc aatctgtgta
     8641 catcaatcaa ttggttattt ataacattct ctatattgtt ttctcctcct ggacataaga
     8701 tgataatatc aaatccaatc ttactaagta aaagcataag aaaaactatt tctttatcaa
     8761 atacaatact cttgtacatg tatataatca atttggggtt tataagtgag taatcaaaat
     8821 tgttcatcat atgaacaaat ttcttatcca tgtgaagtac tgtaaataga ccttttatcc
     8881 tgtcttctcc acttaaacca ttcaaaaaca tacttccttc taacaactct tccacttttt
     8941 ctaaaatcat aatttgttga ttcaatgcta gtgttgatat agaataatct ttattattta
     9001 aaacagagtt tttatctatt acaccttttg aatccaataa aaaggataaa ctaaaagctt
     9061 ctcttgtaaa agcttttgaa aatctatcta catctccatt aaattcaata aatgttgaac
     9121 tttccgaatc tcttaataaa tctaaaaact taatatattc attcttatca ttatatattc
     9181 cgttaatctt agaaagaaaa tttggagctt ctacaatagc atcattaaac ttaaatcctg
     9241 gtcttaactt aagtggctga ttccagtata ttgatatttc atcaacagtt gaacttagaa
     9301 gtagattttt tatttttcta tcttggagtt gccaaggttt tataaatcct gcatcattta
     9361 acaattcctc acttatacgc tttgatgctt ctgctccaac agtaaaagct ttttttacag
     9421 aacttttatc tattttttcc cctaaaataa ccctttcttc aaaagaaact ttttcttcta
     9481 ttatgttggc ttcttcaaac tctatattgt atctttctga ttttaaaata tctatattac
     9541 tctttgaatt tggattaaga tataaaacat caaaacctgc caagtataat attaagagta
     9601 aataaacttc atgttttttt atatctccat aaaatattac ttttggagct tcatttttat
     9661 ttatatctaa agcaccgata taaattttta tccatgccat tattttcact ataaaatttt
     9721 gtttaactct atcattagta aattcttttt cttcttcttt taacactcta tccattgctt
     9781 gctttatttt ataatcaatc tcttcaaatc ctaaaatatt tatatctaca tcttccatgc
     9841 tagttctata tttttgaagt ccacttttaa aataatttat aatatcaaaa tcactaggat
     9901 ttgctataac atcagtaaat aaagcaatat cttttttgct atcaatattt tgttttgcct
     9961 taaatatctc gagattgtac aaaacttcat cttctaaacc aatatacgaa ataaaataac
    10021 ttagatatcc ctttgaagga gtgtaatttc ctctatctga ttggctggag aatatttttt
    10081 caaatacctt tgagtcaccc ataactatcc tcccaattta aatgtttatt cagaactgac
    10141 ccaaaattca tattttgagt cagtctgtgt tcttctaaaa aatttgtttt aaacagtctt
    10201 ttttggtttt accagtctta ttatacattt tacaacttca ttaaccacaa ttacacttgc
    10261 tgctacacat aaaactttta accatgtcat aaaatccatt gctacagagt taaagaaatc
    10321 ttggaatagc tgagtaaata ttacttgtgc aattcctgtc aacactatta tttgtagtgc
    10381 taatttgtta tgtgtaaaat tagggaagat actatctgca ttaaactctc tacagttaaa
    10441 tgcattaaac agtgcactaa aagcaaataa tgcaaacatt actgttccct tatgagcttc
    10501 atcaactcct aagaaattaa atgtagattg taacattagt attgctgtta tataaaatgc
    10561 atttaatact attgtataca ccattgactt agaaattata ttggcttttc tatcaacagg
    10621 ttttctattt aacacatggt ccctaactgg ctctagacct aatgctagtg ctggtggtcc
    10681 atccatgatt atgtttaccc ataataattg tattgtagta aatggcatct cttgtcccat
    10741 aacttgagat attatagcca ctaaaaatgc aattacattt acagttaatt ggaattgaac
    10801 gaatctttgg aagttatcat aaattcctct accccattta atacctttta caatggtact
    10861 aaaactatca tcagttaaaa ttatgtctgc tgcgttttta gatacctctg taccagatat
    10921 acccattgct ataccaacat ctgcttttga aagtgctgga gcatcattta ttccatcacc
    10981 tgttactgca acaacgtctc cgctcttttg cagagctgaa actattctca ttttactatc
    11041 tggtttactt cttgccacga ttgaaatact ttgtatctct tgttttaatt cttcatcatc
    11101 taaagtatct atatatgaag cttcaaccac tctcatatta ttctttaata gacctaactc
    11161 ttcgcctata gctcttgctg tatttatatt atctcctgta agcattttta cagaaacacc
    11221 agctttattt gctgtattta cagactctat aacatctttt ctaagtgggt ctcttatacc
    11281 aacaaaacca ctaaatacta aattgttttc cattgcactt acattcatag catcactttc
    11341 ggcagttact gctgcttctt cttcagcttg tgaaatttct ttatatgcaa accctaaagt
    11401 tctcattgat tttatttgca atttctttat ttcttctaaa atctcatgtt ttatattttc
    11461 agttaatgga actatgtctt ttcctctttg aatatgtgaa cataattcaa gtaagacttc
    11521 tggtgctcct tttgataata gtacatgatg attatcttga cctataacag aagtcattct
    11581 ctttgaatct gaagtaaaag gattttgagc tactatatta gaattttttc taacttgtct
    11641 ataatctacg tttttgtaat ataaaagcaa cgcacattca gtagcacttc ctaaatactt
    11701 aatatcacca tcattgtgct ctacatcagc tgtagagttt atcatacagt tttcttcaaa
    11761 ataactattt atttcctctt ctgaactttc aatatatctg ccatcaacat atgcaacttc
    11821 tacagtcatt ctattttcag ttaatgtacc agttttatct gaacatatta cagaaacaga
    11881 acctatcgtt tcacaagctt cttttttagt aactaatgca tttattttag ccattttttg
    11941 cattgttatt gcaagtgtca tatttatcat agttggaaga ccttctggta ctgctgcaac
    12001 tatcaacgca acacatactg taaatgctgt tttaactggg tctattgatt ctaaaaacgg
    12061 taatattcca gacatatcta catttaatac attcttactt accatttgga aaaccataaa
    12121 tataaataat aatcctgcta ctgcactaga aactttggct atcaaaccac ctaaatttcc
    12181 aagttttatc tgaagtggag tttgctcatc tgtttctgat aagttttgag caattttacc
    12241 catttcacta ttatctccaa ttgaagttac aactagagct cctctaccat aagctacaag
    12301 tgtaccacca aacaccatat ttatctgttt tgcaggtatt ggctcctgta ctactactgc
    12361 tcctttagat tctatctctt ccatagaaac tactacatca cattttttag atacatcatc
    12421 agattctcct gttaacatat cttctctaat tttaaggttt atactttcta taagtctacc
    12481 atcagcaggt accatgtcac cagtttcaat atatactata tcacctggta ccaattcact
    12541 cttagaaatt tgatgtactt ccccatctct caatactttt acttctatgt tttcagtcaa
    12601 cttagaaaga gcctctgccg ctttcttaga acgaccttct gtaatcatac ctattgctat
    12661 acctattgct atagcaccta caatccctat tgcatcatga gtttccccaa ttactgcact
    12721 tactactgct gcaataatca agataactat cattggttca cttaaacttt cctttaaatc
    12781 atcccaaaaa ctaccctctt cttttggagt aaattcattc aaaccatatt tttctcttct
    12841 agtcttgact tctgattctg ataaaccttt ttcaggatta acagataagt cttttgtaac
    12901 ttcttcttta tctacattat aatatttgat atcattatta aattccatat gcacactcaa
    12961 tcctgtctaa attttgcatt acttctttaa gacccgcatc ttttgttcct tcaccaattg
    13021 ctttaaattg ccaactacca tcttttctat atatagctcc tactataaga gtagtttttt
    13081 cagaataatt atcagataga ttatattttg ctatctcttt cttattacct tggtcttcaa
    13141 ctcttatata ggcattttct atcattccaa agtgttgttt tctatctaca caattgtata
    13201 tatttactac aaacaataat ttatgaatat ttgatggaac tttatccaaa ttaactagga
    13261 tttgttcatc atcacctgta ccctcaccag ttagattgtc tcctgtatgc aaaactgact
    13321 tacaggcact ttttagattc ccaaaataaa ttaaatcttt tattccactt aatttgcctt
    13381 cttgattaag cataaataca gatgcatcac aatctatatc agcttgtcct ccaccaaata
    13441 acgatttaaa aaagcctcca ccagattgtt gtacagggtc ccatcctaga cctacaagta
    13501 tgttactcag tcctggatta gattttttta aatctatttt atcacctttt gataaattaa
    13561 tcgacatact aaacctccac aaaattttat ataatataac ctatacatta tttgtatagg
    13621 ttatttatat ttaatatatt atattgctta aattaaacat ttaagccata gtttctacaa
    13681 agtgcttcta agccaccttc aaatcctgct ccaactgcac taaatttcca ttctccatta
    13741 tacttatata tctcacatac tgtaattgca gtttctatac taaattcttc acctaagtca
    13801 tatcttaata tttcttcatc attttcagaa tttaatactc ttatatatga gttgcttact
    13861 tgtccaaaat tttgtcttct ttcagcagcc tcatatattg taactgttac agctatcttt
    13921 tgtatatgct ttggcattgc ctctaaattc actaatattt cctcatcatc tccatcacct
    13981 tcaccagttc tattatcacc taagtgttca actgctccag atggatgttt taagttattg
    14041 tagaatataa aatcttcatc tttttcaact ctttgagatt ctccaaccat gaaaacactt
    14101 acatctaaat cataatcata tccactatca aatgaatttg tatcccatcc tagtccaagt
    14161 tttatatttt taagacctgg atttccttta gttaaatcga ttttttgtcc tttttttaat
    14221 actatagcca tggaaaatat ccccctttag tttattgtta tagttgaaat tataagttta
    14281 ttccaaagtt tccacataat gcagcaagac caccttcaaa gccagaacct agtgcattaa
    14341 acttccattc tccattgtgc ttatatattt cagccactac tatagctgtt tctatactaa
    14401 aatcttctcc taattcatac tttattaact cttcatttgt gtctttatct actattctta
    14461 tataagaatt acttacttgt ccaaaatttt gtcttctttc tgtcgcatca tgaattgtaa
    14521 cagtaaaaga aatcttgctt atttctccag gaatcttaga taaatcaaca actatttgtt
    14581 catcatctcc atcaccttcg ccagttctgt tatctcctag atgctcaact gctccagatg
    14641 tatgttttaa attgttgtaa aacacaaaat ctccatcatt agtaacactt ccacttgctc
    14701 ctgttaaaaa tgctgcggta tctaaatcaa agtcaaatcc accatcatac ttgttagtat
    14761 cccatcctaa acctactaaa atatttttaa gacctggatt tcctttagtt aaatcaacct
    14821 tttggccttt tgataatgtt atacccataa tttatcgctc ccttgttaat tttagctaaa
    14881 caaatcttgc taattattta tctttttctt taatcatatt ataacatttt ttattcattt
    14941 gtgaagaaaa cttgcttgat atttttaaaa tataattaaa aaaatgttta tatttcttac
    15001 tttttacata gattttacat actttattaa atggataaaa gctatgttta atatgtaatt
    15061 atat
//
LOCUS       DAEPXF010000090        14458 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain HB27
            SAMN13376389-rid12479423.denovo.095, whole genome shotgun sequence.
ACCESSION   DAEPXF010000090 DAEPXF010000000
VERSION     DAEPXF010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13376389
            Sequence Read Archive: SRR10518432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 14458)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14458)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001069624.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001069624.1
            Genome Coverage       :: 315x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/15/2021 01:54:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,165
            CDSs (total)                      :: 4,076
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 89
            rRNAs                             :: 10, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 10 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 19 of 69
            Pseudo Genes (incomplete)         :: 44 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 10 of 69
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14458
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13376389-rid12479423.denovo.095"
                     /strain="HB27"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="China: Hebei"
                     /collection_date="2017"
     regulatory      complement(363..446)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01786"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class II; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01786"
     gene            complement(679..1521)
                     /locus_tag="KRL67_002365"
     CDS             complement(679..1521)
                     /locus_tag="KRL67_002365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein"
                     /protein_id="HBH1523239.1"
                     /translation="MFKKKIGVLLASTMLVGALAVGCGSNTGGSGDSGDSSKVSVSGS
                     TSIGPLMETIGEKFQEKNKGVSVEVQQIGSSGGIKNAIEGTSEIGMSSRDLKDEEKSA
                     GLKETKIAIDGIALITNTANPVKNLTMDQVKGIYTGKITNWKEVGGNDAPIVIVSREE
                     GSGTRDGFQEIVGFDSEELTKDAQISDGSGNIKTTVEGNENAIGYISFGYVDEKVNSL
                     KINDVEATDANVIANKYPISRPFILVNKEDGLSEKGKALIDYILSDEGQALVSEKGFI
                     KVNQ"
     gene            complement(1773..3557)
                     /gene="pepF"
                     /locus_tag="KRL67_002366"
     CDS             complement(1773..3557)
                     /gene="pepF"
                     /locus_tag="KRL67_002366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HBH1523240.1"
                     /translation="MGTDRSTIEEKYKWKIDKMYSSKEEIEKDISKVKNLIQEVKEYK
                     GKLSESKENLYKVLNISENASRIVQNLYVYTHMKQHEDTRINDNQGRATKTEMLSTDL
                     GVATSYIVPEILAIEENKLSEYLKDEKLSFYTKYIKDILRDKPHTLSEREEEILAATS
                     ELSTIPENVYDMLAYADMEFPEIEDEEGNKVKLSHSNYSLFIKSKNSRVRKDAFEGEF
                     STYEKYKNTFASTLYGGIKSEIFYAKTRKYNSAIEASLFSDDVSLDVYNNLISAIDKS
                     IPNLNKYVELKKKFLGLNEIHMYDLYVPLTDKFDMDIPYDKAQDIILEALKPLGEEYL
                     KVIKSAFDEGWIDVYDNEGKKGGAYSWGSYDSHPYILMSYKNDLNSLFTLIHELGHSV
                     HSHYSRTSQPFLYSDYKIFVAEVASTLNELLLINYLLENSKSKDETIYLLNYYLEQFR
                     TTVHRQTMFAEFEKIVHQRVESGEPLTADEFTNIYYKLNEKYYGKSAIVDKQIGIEWA
                     RIPHFYSNFYVYKYATGFSAASALSQQILSEGSIAVDRYINFLKSGGSEYPLEQLKSA
                     GVDMTKKESIEEALNVFAELVNKLEKEL"
     gene            complement(3623..4288)
                     /locus_tag="KRL67_002367"
     CDS             complement(3623..4288)
                     /locus_tag="KRL67_002367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HBH1523241.1"
                     /translation="MIDIGSKEIAIRLLLALIIGGIIGLEREKIRQFAGFRTHILIAI
                     GSCIVSITSIQLFFDYNLYTNADPARMPAQVLSGIGFLGAGAILKNKGGVKGLTTAAG
                     MWATACIGIAVGYGYYELAILGGLFVMIALFVLRIINTFLFSQEKNVLILKLSNLDSI
                     PFLYDKFEKNQLIVKNMEIECRNEEYWKVNFFVSYAKRIHLHDVMKEINMIEGVINVD
                     YLD"
     gene            complement(4439..4600)
                     /locus_tag="KRL67_002368"
     CDS             complement(4439..4600)
                     /locus_tag="KRL67_002368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartyl-phosphate phosphatase Spo0E family
                     protein"
                     /protein_id="HBH1523242.1"
                     /translation="MANNELEALKNEIEAVREEINTYIEYPEIFQEELVESSKKIDIL
                     INKYMYLSK"
     gene            complement(4678..5187)
                     /locus_tag="KRL67_002369"
     CDS             complement(4678..5187)
                     /locus_tag="KRL67_002369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gx transporter family protein"
                     /protein_id="HBH1523243.1"
                     /translation="MKTRKMTFLGLMIGYSLALYVLETYIPNPLIVIFPGAKLGLSNI
                     ITLISLMLLGVKDTVIIVTIRVILSSIFAGPLSYLLFSIGGAYLSLLFMYLASKIKDL
                     SLIGISIIGAIGHNIGQLIVASLIIENILAIGYLPFMLMASLVTGMFVGLVSRYTIPK
                     LKHFYSKFY"
     gene            5394..5612
                     /locus_tag="KRL67_002370"
     CDS             5394..5612
                     /locus_tag="KRL67_002370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HBH1523244.1"
                     /translation="MTVYDLKIGEKGFINSIDGDIKLSKRLLALGCINSTEIEVKKVA
                     PLGDPIIIRFRGFDLAIRKSDAKNISLQ"
     gene            5630..7576
                     /gene="feoB"
                     /locus_tag="KRL67_002371"
     CDS             5630..7576
                     /gene="feoB"
                     /locus_tag="KRL67_002371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HBH1523245.1"
                     /translation="MINVALLGNPNVGKTTVFNLLTGSNQYVGNWPGVTIEKKEGFLG
                     KEIKVVDLPGIYAMDTFSNEEKVSKSYLENEDVDVIVNVVDASNLSRNLYLTTQLMQF
                     NKPIVILLNMLDIAESKGVNIDAKKLSEELGVIVVPIIAKKKNGVDRIEEEIKGATQK
                     AFIYQKNFGSETETYKKLESILSRCTKTTYTHKKSISDKIDNIVLNPILAYPIFIGVL
                     FLLFKFTFDWVGGPLQEGFASLIEAYISAPVSNMLSNSSPWFKSLIVDGIIGGVGGTL
                     PFFPLIFTLFFGISLFEDSGYMSRTAFLMDKVMRKVGLSGKAFIPIVMGMGCSSPAIM
                     ATRTLESEKDRRVTALIAPLMTCGAKLPIYALFVAIFFPHNAALVTTSLYLVGIVMAI
                     VVALFLNKTAFKTEAEPFILELPEYRIPTIDALIKNTWNKSKGFLIRVVTVMFAMSVV
                     IWGLSSFNVFGYTEDINVSFLATLGHIISPIFKPLGFDDWRTSVAILSGLGAKEIVVN
                     SLNILYGNLTVVLPTIFNGVTAYTFLIFTALYTPCIAALATMKKEYGNKMMFTSFAYQ
                     FILAWIMAFIVKSIGGVLFMGNSIMEALIGGIIIVLALFILFNKFKSTKNGNVCSGCS
                     ICSGCPSANSCSSKEESNKKSNTH"
     gene            complement(7736..8791)
                     /locus_tag="KRL67_002372"
     CDS             complement(7736..8791)
                     /locus_tag="KRL67_002372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HBH1523246.1"
                     /translation="MTIQDYMLETPVRMREIISNADSLFNEVKRTNLKKIIITGSGTS
                     YHSGVQVQPYLQNLLDIDVVKMYPFMITEDTFKFDNENTLVVGVSQGGSSYSTYNAMK
                     LAEDKGCKIASMAGCKNALIDEISDYILTVNCGEEKSGAKTKGYYCTKLNLMLLGLQI
                     AREKGIISSEKYNEEINKILDAINRFEAVYKLSKQWIERNKEKLVNSKEIRIIGHSDI
                     YGDTLEAALKLLETMRIPVTGYEFEEFIHGIYNAINSDSTIFILDTGKEPRVTKMIDV
                     LSGWTENVFAIGRDVTENDKNLKIDITDNPYYQTFNFIVPIQLICGEIPTLRGVDPSV
                     PKDTRFHMKLGSKKLNK"
     gene            complement(8839..9639)
                     /locus_tag="KRL67_002373"
     CDS             complement(8839..9639)
                     /locus_tag="KRL67_002373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HBH1523247.1"
                     /translation="MNKLTKKELRSMFWRSFALQGAFNYERMQNLGYCYSMLPAIKKL
                     YNKKENQAKAIERHLEIFNTTTVVVPAILGITAAMEEENANNPEFDESSISAVKTALM
                     GPLAGIGDSLFWGTFRIIAAGVGVSLAKEGNIFGPLLFLLLYNIPAFALRIFGLKYGY
                     QVGVNSLERIQREGLMEKIMSMATTVGLFVVGGMVATMLSITTPLKFNLNGAEVILQD
                     ILDKIIPNMLPLTFAFVIYYMLKRKVSVTKLTIGTIVTGIALHAIGLL"
     gene            complement(9632..10423)
                     /locus_tag="KRL67_002374"
     CDS             complement(9632..10423)
                     /locus_tag="KRL67_002374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBH1523248.1"
                     /translation="MLVQATMLAIIAGVGILDGRIFGQSMLDRPIVTGMLVGLVLGDI
                     KSGIMIGAQLELIWMGIAGIGAATPPDVVTGGVLGTAFAILSGNGAEVALAVAVPVAV
                     LAQSLGVLVRIINSYFSQKAVFYAKQADFKKVTIMMWLPVILFFLSTSVPTFLAIMIG
                     ADKVTALINAIPKVILDGLGVAGILLPAVGFALLMDMLLSKKMSVFFFIGFLLASYGG
                     LDITAIALFGVCVAFVLNFYINNKENNQNPQLAINTLTEGEIDFE"
     gene            complement(10463..10885)
                     /locus_tag="KRL67_002375"
     CDS             complement(10463..10885)
                     /locus_tag="KRL67_002375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIA"
                     /protein_id="HBH1523249.1"
                     /translation="MKKFLIATHGELSRELVDTSKLIIGSLTNIEYFCMTTDKSADDA
                     EKEIRSILSNNKDSEEFVVLTDVFGGSVANICTNLLLQGFKFELLTGVNLPMLLSILL
                     LVEEDTKLIVKSGLEEAKNGIVYVNELLENQNKIKNVM"
     gene            complement(10901..11380)
                     /locus_tag="KRL67_002376"
     CDS             complement(10901..11380)
                     /locus_tag="KRL67_002376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBH1523250.1"
                     /translation="MVSLMRIDDRLIHGQVAYGWATSLGINVILVANDEAKNDQMKAM
                     SLNLAKPSNVTLYIRGVEESGEIVEKFASSKKSNVLVLVKNTKDALVVAKNSGGVLKE
                     INVGGLRYEEGKKKLTDLVAVDDDDINNFKQIGDLGIDIEFRMLPRDKKKQLSDLIK"
     gene            complement(11512..14268)
                     /locus_tag="KRL67_002377"
     CDS             complement(11512..14268)
                     /locus_tag="KRL67_002377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBH1523251.1"
                     /translation="MRDEILSFVNECVLFNNFDELKKESGIITEDIVKKFNLSRTQSS
                     RLLNDLVKSDNLIKINSRPVIFLPKNKINDVVGPTKKSIYENLDELKNECKLLKQETV
                     FNSIIGYDNSLKEVIEQIKTAVLYPNGGLTIMLTGESGVGKTFLAKIIYKYSVSSNVL
                     EKNSPYNVLNCAQYYNNLELLSSLLFGHVKGAYTGANESRAGLIEQSDGGVLFLDEVH
                     RLNSEGQEKLFTFMDTGTFSRIGENGVIRKARVRLIFATTESLSEFLQTFLRRIPINI
                     YVPNIEERGTIEKRKIVEHFIYEESKVLGLPIEITQITLNAILKIKFKGNIGECKNVI
                     KYACGRAYAKNQRRNGNIFVTLQDLPRNLYIENVDIFQFQSGKSKSIIFSPKQFSIEM
                     SNGRNKRNIIYQSFIKCLDYFDRLEKNEITKEKFSKKSSNLMIRLMDNLVFSNNDENN
                     NVLMELIISIMQDIFRNLEVNNNIKYDGNNVLAFSSYLYLKDESYQLSDEYKALENKL
                     LIYLKKESYKEYSIISKISTLITNRLDMYLNNVDTIIMIIFIKSTIIRMKFNQVNATI
                     IAHGYATASSMANVCNRMLGKNIFTAIDMPIDATIADVSKQMSRYLEGDRIKNGLIVL
                     FDMGSLNLIYEDLKNKINSPMLFIDQISTISALEVGNLLMQGKNIEEIAEIMDEETKP
                     NIQLYYPQKNKEYAIISSCFTGIGTAIKIQHLLSESLIGIVDVKIIPYDYDSLVSMGD
                     KEPVFEMYDVIAIVGTANPNVNGVDFILLEDIISGKGEDDVFRIFSRVVENEKIKNIN
                     DSIVKNFSLIRVIDSLTILDSKKIIERIEEGINAIENIQKKKLSNDKKISLYVHLSCM
                     IERLVRQTEIEEYTDMDLLIKNHHSEVKLIKNAFSVLEKSYSVQIPISEIGYIYNIIY
                     GLPQ"
BASE COUNT         4715 a         2477 c         1636 g         5630 t
ORIGIN      
        1 aagggaaatt ttttaaaaag tataatacta ttataataag aaaataaagt ttttataaac
       61 attgagatgc agaatataaa ccttttgtaa caatatattt accaaaataa tacaatttat
      121 tttatattat aaccatataa atcatataaa tcaatactca aactagccaa tagacaagtt
      181 tctagaaaaa taataaattt ataagtaaag atgactttaa gctccattct ccaacaaatg
      241 gctactttaa aagacaaaat ttcacctatc aataaaaatc ttaacgacta atactcttat
      301 tttcaaattt tgcaacattt ttgttaattt taaaacaaaa aaagactatg caatataaaa
      361 attatattca tagccggttg caccactaac tccatatatc tccagatgcc caataataag
      421 atatacttct cagtttctaa aatattttta ttaaaaatct tttatatatt ttcataaatt
      481 tcttttttat tctaacattg taatattatt ttgtcaatat ttccatattt ttagatataa
      541 gatactaaaa tatctatttt atctaaagat atttatattt ctacattcaa taatagacaa
      601 taccctgcaa gttaaagcat catttttatg agcttatccc acagggtatt atatgtttac
      661 tttatattat aatttatgtt attgattaac tttaataaat cctttttctg aaacaagtgc
      721 ttgtccttca tcacttaata tataatctat taaagcttta cctttttctg acaaaccatc
      781 ttctttatta actaatataa atggtcttga tattggatat ttattagcta ttacatttgc
      841 atcagttgct tcaacatcat ttatttttaa actatttact ttctcatcaa catatccaaa
      901 tgatatatat cctatagcat tttcatttcc ttcaactgta gtttttatat ttcctgaacc
      961 atcacttatt tgtgcatctt tagttagttc ttctgaatca aaccctacaa tttcttggaa
     1021 tccatctcta gttcctgaac cttcttctct tgatactatt actatcggtg catcatttcc
     1081 tcctacttct ttccagttag taatctttcc tgtatatata cctttaactt ggtccattgt
     1141 cagatttttt actgggtttg cagtattagt tatcaatgct ataccatcta ttgctatttt
     1201 agtttccttt aatccagcac ttttttcttc atcttttaaa tctcttgaac tcattcctat
     1261 ttcagatgtt ccttctatag cattttttat acctccagaa gaaccaatct gttgtacttc
     1321 tacactaaca cctttatttt tttcttgaaa cttttctccg atagtttcca taagaggacc
     1381 tattgaagtt gaacctgaaa cagaaacttt gcttgaatct cctgaatcac ctgaacctcc
     1441 tgtgttagaa ccacatccta ctgctaaagc tccaactaac attgtactag ctaaaagaac
     1501 gcctattttc tttttaaaca ttttacttac ccccgcttat attttaattt tttgttgtca
     1561 tttttctata ttttaataat acagattctt tgttaaatga atattagagc agggttaagt
     1621 aaatgtaaat attgttaaat acatgtaaaa acttaatttt atatgattta ttcttaatta
     1681 gattaacttt ttaaagttac tttaggcaga tataaacata atacaactca tattatcaga
     1741 agtcttcatt gaagctatca acctatcaca tcctataatt ctttttctag tttattaacc
     1801 aattcagcaa atacatttaa ggcttcctct atagattctt ttttcgtcat atctacacca
     1861 gcagatttta attgttctag tgggtattct gaccctccac tttttaagaa gtttatatat
     1921 ctatctactg caatacttcc ttctgaaagt atctgttgac ttaaagcact tgcagcagaa
     1981 aaccctgtgg catacttata tacatagaag tttgaataaa aatgagggat tctagcccat
     2041 tctattccta tttgtttatc tactattgca gattttccgt aatacttttc atttaatttg
     2101 tagtatatat tagtaaattc atcagcagta agtggttctc cactctctac cctttgatgt
     2161 acaatttttt caaattcagc aaacatagtt tgtctatgaa ctgtagttct aaattgttct
     2221 aaataataat ttaataagta tatagtttca tccttagact tagaattttc taataaataa
     2281 tttataagaa gtaattcatt taatgtagat gcaacttctg caacgaatat cttatagtcc
     2341 gaatataaaa atggttgact tgttcttgaa taatgactgt gaacagagtg ccctaattca
     2401 tgaattaaag tgaatagaga attaaggtca tttttatagc tcattaatat ataaggatgt
     2461 gaatcataac ttccccaaga ataagctcct ccttttttac cttcattatc atatacatca
     2521 atccaccctt catcaaaagc actttttatg accttcaagt attcttcgcc taaaggtttg
     2581 agtgcctcta atatgatatc ctgtgcttta tcatatggta tatccatgtc aaatttatct
     2641 gtcaaaggaa catacaaatc atacatgtgt atttcattca atcctaaaaa tttcttttta
     2701 agctcaacat atttatttag attaggaata cttttatcta tagctgatat aagattattg
     2761 taaacatcta aacttacatc atctgaaaat aaagacgctt ctattgctga attatacttt
     2821 ctagtttttg cgtaaaatat ctctgatttt attccaccat aaagcgttga ggcaaatgta
     2881 tttttatatt tctcatatgt tgaaaattca ccttcaaaag cgtcttttct aactctacta
     2941 ttcttacttt ttataaataa agagtaattt gaatgagaaa gcttaacctt attaccctct
     3001 tcatcttcta tctcaggaaa ttccatatct gcatatgcta acatatcgta tacattttct
     3061 ggaattgtcg ataattctga tgtagctgca agtatttctt cttctcgttc actcaaagta
     3121 tgtggtttat ctctcaatat atcctttata tattttgtat aaaaagatag cttctcatct
     3181 tttaaatatt cacttaactt attttcttct attgccagta tttctggaac tatataagaa
     3241 gtagccacac ctaaatcagt agataacatt tctgtcttag tggctcttcc ttgattatca
     3301 ttaattctag tatcttcatg ttgtttcata tgagtatata catataaatt ttgaacaatc
     3361 ctagatgcat tttcagagat atttaatact ttatataagt tttccttgct ctcagaaagt
     3421 ttccctttat attcttttac ttcttggata agatttttta ctttgcttat gtctttttca
     3481 atttcctctt tactggagta catcttatct atcttccatt tatacttttc ttctattgta
     3541 cttctatcag ttcccatatc atacacctct attttaattt gttttatatt ttattcactt
     3601 tacaaaatat attgtatata ttttagtcca aataatctac atttattact ccttctatca
     3661 tatttatttc tttcataacg tcgtgtaagt gtattctttt agcataagat acaaaaaaat
     3721 taaccttcca gtattcttca ttacgacatt ctatttccat attttttacg ataagttgat
     3781 ttttttcaaa tttatcatat aaaaaaggta tagagtctaa attgcttaat tttaatatca
     3841 atacattctt ttcttggcta aataaaaatg tattgattat cctaagcaca aataatgcta
     3901 tcattacaaa taatccacct agaattgcca attcataata accatatcca actgctatac
     3961 caatacatgc agttgcccac atcccagcag ctgtagtgag cccttttact ccacctttat
     4021 tttttaaaat tgctcctgca cctaaaaaac caattccaga taatacttga gcaggcattc
     4081 ttgctgggtc agcatttgta tacaaattat aatcaaagaa taattgaatc gaagttattg
     4141 aaacaataca ggaacctatt gcaattagta tatgtgtcct aaatcccgca aactgtctta
     4201 ttttttctct ctctaagcct attattcctc ctattattag tgctaatagt aaccttattg
     4261 ctatctcctt acttcctata tcaatcataa tataaaaccc tcctgactta attacaactt
     4321 atgtcctaca gataatttac acatcagaaa tactatttta tatcttaata ttattattaa
     4381 tatatgtaaa atatagccat ctgttaattt ttgatacaaa aaaacccaca actgtgggct
     4441 atttgcttag gtacatgtat ttatttatta atatatctat tttcttactt gattctacta
     4501 actcttcttg aaatatttct ggatattcta tgtatgtatt tatctcttcc ctaactgctt
     4561 caatctcatt ttttaacgct tctaattcat tgttcgccat aataatcacc actattacaa
     4621 atttgtatat ttttatatac attttatcaa acaatattat attttgtcaa tattatgtca
     4681 ataaaactta ctataaaaat gttttaattt aggtattgta tacctggata ctaaacctac
     4741 aaacatacca gttactaatg atgccattaa cataaatggt aagtacccta tagcaagtat
     4801 attttctatg attaaacttg caactattaa ttgacctata ttatgcccta ttgcacctat
     4861 aatgcttatt cctattagtg ataaatcttt tattttactc gctaaataca taaataaaag
     4921 actcagataa gctcctccta tactaaatag tagatatgat aaagggcctg caaatattga
     4981 tgacaaaatt accctgatag taacaattat aacagtatct ttgacaccta acaacattaa
     5041 tgaaattaaa gttataatat tgctcaaacc caattttgcc cctggaaata taactataag
     5101 aggatttggt atatatgtct ctagtacata taaagctaaa ctatacccta tcataagacc
     5161 taaaaatgtc atttttctag ttttcattaa gttaactcct ccaaatttta tctatcatct
     5221 tacattataa tacaaaatat ataaaaacac ctaatttaga ccaaaatatt ttttaatcta
     5281 tttagaataa atattagtta tttcaatgtg taattgtctc tataattctt gactttttgt
     5341 atcatacttg ttacaatttt aatgataatg aatttcatta ggaggtgtta ataatgactg
     5401 tttacgattt aaaaatagga gaaaaaggat ttattaatag tatagatgga gatattaaat
     5461 tatctaaaag attgcttgca cttggatgta taaatagtac tgaaatagaa gttaaaaagg
     5521 ttgccccact tggagaccca attataatta gatttagagg ctttgacctt gcgattagaa
     5581 aatctgatgc taaaaatatt tctttacaat aatagaggag gagctacccg tgattaatgt
     5641 agcattatta ggaaacccaa atgtcggtaa aactaccgtt tttaatctac ttacaggttc
     5701 aaatcaatat gtaggtaact ggcctggtgt tactatagaa aaaaaagaag gatttcttgg
     5761 aaaagagata aaggttgtcg atttacctgg tatatatgca atggatactt tctcaaatga
     5821 agaaaaggtt tctaaatcat atttagaaaa tgaagatgtt gatgtaatag taaatgtagt
     5881 tgatgcttct aatttatcaa gaaaccttta cttaacaact caacttatgc aatttaacaa
     5941 acctatagtt atattgctta atatgttaga cattgcagaa tctaaaggag ttaatataga
     6001 tgctaaaaaa ttaagtgaag aacttggagt aattgttgtt ccaattatag caaaaaagaa
     6061 aaatggtgtt gataggatag aagaagagat taagggagct acacaaaaag cttttatata
     6121 tcagaaaaac tttggaagtg aaacagaaac atataaaaaa ttagaatcca ttttatcgag
     6181 atgtacaaaa actacatata cacataagaa atctatcagt gataaaatag ataatatagt
     6241 tttgaatcct atattagcat atccgatatt tattggtgta ttgttcctct tatttaaatt
     6301 tacctttgat tgggtcggag gaccactaca agagggattt gctagtctta tagaggctta
     6361 tatttctgca cctgttagta atatgctatc taactctagc ccatggttta aatcacttat
     6421 agttgatgga ataattggag gtgttggtgg tactttacca ttcttccctt taatatttac
     6481 attattcttt ggtatatcgt tgtttgaaga tagtggatac atgtcaagaa cagcattttt
     6541 aatggataaa gttatgagaa aggttggtct atctggtaaa gcttttatac ctatagttat
     6601 gggtatggga tgttcttctc cagctataat ggcaactaga acattggaaa gcgaaaaaga
     6661 tagaagagtc acagctctta ttgcaccact tatgacttgt ggagcaaagc tacctatata
     6721 tgcactattt gtagctatat tctttccaca taatgctgct ttagtaacta cgtcactgta
     6781 tttggtgggt atagttatgg caattgtagt tgcattattc ttaaataaaa cagcctttaa
     6841 aacagaggct gaaccattta tattagaatt accagaatat agaatcccta ctatagatgc
     6901 acttattaaa aatacttgga ataaatcaaa aggtttcttg ataagagttg ttactgtcat
     6961 gtttgcaatg tcagtcgtaa tttggggact ttcttcattc aatgtttttg gatatacaga
     7021 agatataaat gtaagcttct tggctactct aggtcatatt atatctccta tattcaaacc
     7081 tcttggtttt gatgattgga gaacttcagt tgctatttta agtggtcttg gagcaaaaga
     7141 aatcgtagta aactctttaa atatactata tggtaatcta actgttgtct taccaacaat
     7201 atttaatgga gttactgcat atacattctt gatatttact gctttatata ctccatgtat
     7261 agctgcactt gcaactatga aaaaagaata tggcaataaa atgatgttta catcatttgc
     7321 gtatcaattt atactagcat ggataatggc ttttatagtt aaaagtatag gtggagtact
     7381 atttatgggt aattcaataa tggaagcctt gataggtgga ataattatag tccttgcact
     7441 ctttattctg tttaataaat tcaagtctac aaaaaacgga aatgtctgta gtggatgttc
     7501 tatttgctct ggatgtccaa gtgctaattc ttgctcaagt aaggaagaaa gcaataaaaa
     7561 aagtaataca cactaatata taaatatttt agtttaaaat aagataaaag tgggtgtctc
     7621 aaaattaact ttttagttca tgaggcactc ttttttatac gaaatcaaat atattttcat
     7681 catttcaaca gccaaaaaga ggcttatctc tattttgaga caacctcttt ttttgttact
     7741 tattcagttt cttgctacct aacttcatgt ggaacctagt atcttttgga acacttgggt
     7801 ctacgcctct taatgtaggt atttccccac atattagttg tataggtact ataaaattaa
     7861 atgtttgata gtaaggatta tctgtgatat caatctttaa atttttatca ttttctgtaa
     7921 catctctacc tatagcaaat acattttccg tccaaccaga aagtacatca atcatcttag
     7981 ttactctagg ttcttttcct gtatctagta taaatatagt agaatcacta tttatagcat
     8041 tatatatacc atgtataaac tcttcaaact catacccagt tacaggtatt ctcattgttt
     8101 ccaataattt taatgctgct tctaatgtat ctccatatat atcagagtga cctattattc
     8161 ttatttcttt agaattaact aacttttctt tgtttctttc aatccattgt ttagatagct
     8221 tatacactgc ttcaaatcta ttaattgcat ctaatatctt atttatttcc tcattatatt
     8281 tttcactaga tattatacct ttttctctag ctatttgaag acctaataac attaagttta
     8341 actttgtaca ataatatcct tttgttttag ctccagattt ttcttcacca caatttactg
     8401 ttaatatata atcagaaatc tcatctatta gagcattctt acaacctgcc atagatgcta
     8461 ttttacaacc cttatcttca gctaacttca tagcgttgta tgtagaataa ctactcccac
     8521 cttgagatac acctactact agggtatttt cattatcaaa cttgaatgta tcttcagtta
     8581 tcataaatgg atacattttc acaacatcta tatctaataa attttgtaaa tatggttgca
     8641 cttgcactcc tgagtgatag ctagtacctg aacctgttat tataatcttt tttagatttg
     8701 ttctcttaac ttcattaaat aagctatctg cattagatat aatttctctc attctaactg
     8761 gtgtttctaa catataatct tgtattgtca ttttatacac cttccttatc tatatattca
     8821 ataaattata attaatttct ataacaaacc tatagcatgt aaagcgattc ctgttactat
     8881 agtgcctata gttaattttg ttacacttac ctttctcttt aacatgtagt atattacaaa
     8941 tgcaaaggtt aaaggtaaca tattaggtat tattttatct agtatatctt gtaaaattac
     9001 ttctgcacca tttaagttga atttcagagg agtagttata ctcaacatag ttgcaaccat
     9061 tcctcctaca acgaataaac caacagttgt agccatagac attattttct ccataagtcc
     9121 ctctctttgt attctttcaa gagaatttac ccctacttga taaccatact taagtccaaa
     9181 tattctaagg gcaaatgctg gtatattgta taatagtaag aataatagtg gtccaaagat
     9241 attaccttct tttgcaagtg atactcctac tcctgctgct ataatcctaa atgttcccca
     9301 gaataatgaa tcaccaattc ctgcaagagg tcccataaga gctgttttaa cagcactaat
     9361 tgaactttca tcaaattctg gattattagc attctcttct tccatagctg ctgttatacc
     9421 taatatagca ggaactacta ctgtcgttgt attaaaaatc tccaaatgcc tttctattgc
     9481 ttttgcttga ttttctttct tattgtatag cttctttatt gcaggtaaca ttgaataaca
     9541 gtaacctaag ttttgcattc tttcgtaatt aaatgcacct tgaagagcaa atgaacgcca
     9601 aaacatactt cttaattctt tttttgttaa tttattcaaa gtcaatctct ccttccgtta
     9661 aagtattgat tgctaattga ggattttgat tattttcttt gttattgata taaaagttta
     9721 gtacaaatgc tacacatact ccaaataaag ctatagcagt tatatctaat ccaccataag
     9781 atgctaacaa aaaccctata aagaaaaata cagacatctt ttttgaaagt aacatatcca
     9841 taagcaatgc aaaacctact gctggcaaca atattcctgc aacacccagt ccgtctaata
     9901 taactttagg tattgcattt atcaatgctg taactttatc agcaccaatc ataattgcta
     9961 aaaaggttgg aactgatgta cttaaaaaga ataatattac tggcaaccac atcattatgg
    10021 taactttctt aaaatcagcc tgtttagcat agaaaactgc cttttgagaa aagtacgaat
    10081 ttattattct aactaatact ccaagagatt gtgctaagac tgctactggt actgctactg
    10141 ctaaagcaac ttctgcacca ttacctgata atatagcaaa agctgtacct aatacacctc
    10201 ccgttactac atctggagga gtagctgcac ctattcctgc aattcccatc caaattaatt
    10261 caagctgtgc accaatcata attcctgatt ttatgtctcc taaaactaat cctactaaca
    10321 tacctgtaac tattggccta tcaagcatag attgaccaaa tatacgccca tctagtatac
    10381 caactcctgc tataatagct aacatagtag cttgtactaa catattgaac ctccatttta
    10441 acttagttta atttacatta aattacatta catttttgat tttgttttga ttttctaata
    10501 actcatttac ataaacaatt ccgttttttg cttcctctaa tcctgattta actattagtt
    10561 ttgtatcctc ctcaactaat aaaagtatag ataaaagcat tggtaaattt actcctgtca
    10621 ataattcaaa tttgaaccct tgtaaaagta agtttgtaca tatgtttgcc acacttccac
    10681 caaacacatc tgttaagaca acaaattctt cactatcttt attatttgat aatatacttc
    10741 taatttcttt ctcagcatca tctgctgatt tatccgttgt catacaaaaa tactcaatat
    10801 tagttaatga accaattatt aatttacttg tgtcaacaag ctctctggac aattctccat
    10861 gtgttgcaat tagaaatttt ttcattctct ttctcctaaa ttattttatt aaatctgata
    10921 gttgtttctt tttgtctcta ggtaacattc taaattctat atcaatccct aaatcaccta
    10981 tttgtttaaa gttatttata tcatcatcat caacagcaac taaatctgta agtttcttct
    11041 tcccttcttc atatcttaat cctcctacat ttatttcttt taatactcct cctgaatttt
    11101 tagctacaac taaagcatct ttggtatttt ttactaacac taatacattg cttttttttg
    11161 aagaagcaaa tttttctact atttctccag attcctctac cccccttata tataaagtta
    11221 cattgctagg tttcgctaag ttcagtgaca tagctttcat ttggtcattt tttgcttcat
    11281 catttgcaac tagtattaca tttataccta gtgatgttgc ccatccatat gcaacctgac
    11341 catgtatcaa tctgtcatca attctcatca aagataccat tttccccaaa tcctccataa
    11401 tacattaaaa tttttattgt ttatactctc atttatctat aaaagcaaaa agtatgccaa
    11461 atactggcat acttttttaa atttcattca cttattttgt aatcttgtat tttactgtgg
    11521 tagaccatag ataatattat aaatatatcc tatctctgaa attggtattt gcacactata
    11581 actcttttct agcacactaa atgcattttt tattagtttt acttcactat gatggttttt
    11641 gattaacaaa tccatatcag tatactcttc aatttcagtt tgtctaacta atctttcaat
    11701 catacaactt aagtgaacat ataaagaaat ctttttatca tttgaaagtt ttttcttttg
    11761 aatattttct atagcattaa ttccttcttc tattctctct attatctttt ttgaatctaa
    11821 aatagttaga ctatctatta ctctaattaa tgaaaaattc tttacaatag aatcatttat
    11881 gttctttatt ttttcatttt ctactactct gctaaatatt ctaaaaacat catcttctcc
    11941 ttttcctgat attatatcct ctaataatat aaaatctacc ccattcacat ttggattggc
    12001 agttcctact atagcaatca catcatacat ttcaaaaact ggttctttat ctcccataga
    12061 gacaagacta tcataatcat aaggtattat tttgacatca actattccaa ttaaactttc
    12121 ggaaagtaga tgttggattt ttatagcagt acctattcct gtaaaacaag atgatattat
    12181 agcatattct ttattttttt gaggataata caattgtata ttaggtttag tttcttcatc
    12241 cataatttct gcaatctctt caatgttttt accttgcatt aataaatttc ctacttctag
    12301 tgcagaaata gtggatattt ggtctataaa taacattggg ctatttatct tattctttaa
    12361 atcttcatat ataagattaa gagagcccat atcaaataat acaattaaac cattctttat
    12421 cctatctccc tctaggtatc tagacatttg ttttgataca tcagcaatag tagcatctat
    12481 aggcatatca atagctgtaa atatattttt acccaacatc cgattacata cattcgccat
    12541 actactggca gtagcatatc catgtgctat aatagtcgca ttaacttgat taaatttcat
    12601 tcttataatt gttgatttaa taaatataat cattatgatt gtatctacat tattaagata
    12661 catatccaat ctattcgtta ttaatgttga tattttactt attatagagt attctttgta
    12721 actctctttt tttaaatata ttagtaattt attttctaaa gctttatact catcagacaa
    12781 ttgataagat tcgtccttca aataaagata agacgaaaat gccaatacat tattcccatc
    12841 gtattttata ttgttattaa cttctaaatt tctaaatata tcctgcatta ttgatattat
    12901 aagctccata agtacgttat tattttcatc attatttgaa aagactaaat tgtccattag
    12961 tctaatcatt agattagaac tctttttact aaatttttcc tttgttattt catttttttc
    13021 tagtctatca aagtaatcta aacattttat gaaactttga tagattatat ttcttttatt
    13081 tcttccattt gacatttcaa tactaaattg tttaggagaa aagataatgc ttttgctttt
    13141 accactttgg aattgaaata tatctacatt ttctatataa agatttctag gaaggtcttg
    13201 aagagtaaca aaaatatttc catttcttct ttgatttttt gcataagctc gtccacatgc
    13261 atattttatt acatttttac attcacctat attccccttg aattttatct ttaaaatcgc
    13321 atttaatgta atttgagtta tctctatagg caaaccaagt accttacttt cttcataaat
    13381 aaaatgttct acaatctttc ttttttcaat agtccctctt tcttctatat tgggaacata
    13441 aatatttatg ggtattcttc ttaaaaaggt ttgtaaaaat tcagatagac tttctgttgt
    13501 agcaaatatt aatcttaccc tagcttttct aattactcca ttctctccaa tcctagaaaa
    13561 cgtacctgta tccataaatg taaataactt ttcctgacct tcactattta atctatgtac
    13621 ttcatctaaa aataaaacac caccatctga ttgttctatt agacctgctc tagattcatt
    13681 tgctccagta tatgctcctt ttacatgccc aaataataga ctagacaata actccaaatt
    13741 attataatac tgcgcacagt ttaacacatt gtatggagaa tttttttcta atacattact
    13801 gcttacacta tatttgtata ttatcttggc taaaaaagtt tttcctactc cactttcacc
    13861 tgtcagcatt attgtaagtc cgccatttgg ataaagtaca gcggttttta tttgttcgat
    13921 tacttctttt aagctattat catatcctat gatactatta aacacagtct cttgctttaa
    13981 taatttacac tcatttttaa gttcatctaa attttcataa atactttttt ttgtaggtcc
    14041 tactacatca tttattttat ttttaggtaa aaaaataaca ggtctactgt ttatctttat
    14101 aagattatca gattttacta aatcatttaa taatctactt gattgagttc tacttaaatt
    14161 aaactttttt actatatctt ctgttattat gccactttct tttttaagtt catcaaagtt
    14221 gttaaataat acacattcat ttacaaaact taaaatttca tccctcatgt tcaacctcca
    14281 atacaaatat caataatact ataatattag tatatcattt atatatttat gaaaaagata
    14341 attatattcc tatcttgatt ctcttttgta aggttgtaaa atcaaacttt ttataagtat
    14401 aaaatctaga ttttttaaca taatctcaag gttcttatct ttccatatat ctgacata
//
LOCUS       DAEPXF010000091        14408 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain HB27
            SAMN13376389-rid12479423.denovo.096, whole genome shotgun sequence.
ACCESSION   DAEPXF010000091 DAEPXF010000000
VERSION     DAEPXF010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13376389
            Sequence Read Archive: SRR10518432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 14408)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14408)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001069624.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001069624.1
            Genome Coverage       :: 315x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/15/2021 01:54:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,165
            CDSs (total)                      :: 4,076
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 89
            rRNAs                             :: 10, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 10 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 19 of 69
            Pseudo Genes (incomplete)         :: 44 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 10 of 69
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14408
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13376389-rid12479423.denovo.096"
                     /strain="HB27"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="China: Hebei"
                     /collection_date="2017"
     gene            306..1019
                     /locus_tag="KRL67_002378"
     CDS             306..1019
                     /locus_tag="KRL67_002378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015359980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF434 domain-containing protein"
                     /protein_id="HBH1523252.1"
                     /translation="MNKISKRGFDDSDKRWFSSKELTRLIKAKEEIEWLINRDYKVDP
                     VVTFVGDRYQFSIRQRDALKRAVCTDEKNIIRQSKRLSLDKIKENTVNIDGFNLIISI
                     EVALSGGTLVIGSDGNIRDLAGLRGTYKIIDKTEEALNLIGKFFNKYNAQSLKFYLDS
                     PVSNSGNLKYGILEYAKIWGIETEVELVKNADVVLEKLDRVVSSDAVIVDKCISYFNV
                     ARGIIEEYIKDCNIVNLNK"
     gene            1135..2004
                     /locus_tag="KRL67_002379"
     CDS             1135..2004
                     /locus_tag="KRL67_002379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HBH1523253.1"
                     /translation="MRGWICVKLTKLNIKKYRAPIYKYALANVNLRSAKSTNSSIITV
                     IPQGAKMEVLDEEDDWIKVMYNSQEGYVYKDLVSVSEYAWSNLNLRENKSTTSNIITV
                     IPEKSRVEVLQVDGDWSKVVYDDKTGYVFNYFLSTDGNKPNELDYKYFYTDMIKFVNE
                     NNIKSTSDYLIVTDLRNKYTYIFKKDNGGWGQLYKWQCTIGKPETPTITGIFYISGRK
                     PSFGTDEYSVKYATRIKGGYYYHSVLYDSTGSYIIDGRLGEALSHGCIRLSTENAKWI
                     YDNIPDTTTVIIH"
     gene            complement(2159..3304)
                     /locus_tag="KRL67_002380"
     CDS             complement(2159..3304)
                     /locus_tag="KRL67_002380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HBH1523254.1"
                     /translation="MSIYYVPPINLLGKGCLNEAKDSIKSLGTKKAFVVSDKFLVSNG
                     TVKKVTDILSQIDVDFVVYDEIKQNPTVTNVNKGLKILKSENCDFVITIGGGSPQDCG
                     KAISILATNGGDIRDYEGINKTSKKCLPIIAITTTAGTSAEVTINYVITDEDRHVKMI
                     MVDTNSLATMTVNDPELMISKPSNLTAATGMDALTHAIEAVVANGAYDVTDSTALYAI
                     KQIFKYLPRAVKNGNDIEAREQMCYACFLNGIAFSNAGLGNVHAMAHQLGGLYDLPHG
                     VCNAMLLPIVEEENAKSAPAKFRPIAEVIGMDVNNKSDKECVDFVIDKIKALSEEVGI
                     PKSLKDVGVDNPNFELLAENSMKDACAGANPVFFDKEKIIELFMKIS"
     gene            complement(3637..4413)
                     /locus_tag="KRL67_002381"
     CDS             complement(3637..4413)
                     /locus_tag="KRL67_002381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HBH1523255.1"
                     /translation="MTSDNHRPTARILDILEALAESENGYTLTEIADAINAPKSSIFP
                     IVHTLNARKYITIDKNTSKYSIGIRSYTLGSSFFEKRTIFNYIIDEMTNIVNSCSETC
                     QLGILDNNEVLYIGKVDSPEPIRLISYVGKKIPANCTGLGKALLCDFDKEKLISLYPD
                     GLKGFTEKSITDFDVLYDQLLEVQRTQIASECEESMEHLKCLAVPIRKNGCIRAAVSV
                     TLPLFRANDEKIELIKKLLLNAQKNIQSMMEERNEDIVIN"
     gene            5001..5975
                     /locus_tag="KRL67_002382"
     CDS             5001..5975
                     /locus_tag="KRL67_002382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-3-deoxygluconate permease"
                     /protein_id="HBH1523256.1"
                     /translation="MIMDFIKKIPAGMMIVPMFIAAFINTFCPDIVQIGSFTTAVFSS
                     VGSATIIGVQLVCLGTQLQFREMPKVLKRGGILLGSKFAIGALIGILIGKLFGMDGVL
                     GLTTLAVISAVTNSNGSIYLSLMTSYGDETDCATMSLLTLNDGPFFTLIALGTSGLAN
                     VPLLSLLAAIVPILVGMIIGNFDKKMKEFLEPGCNLLVPFVGFALGSSINLASILKGG
                     LSGVLLGLISVFVGGIFIVFCDKFIGKRPGYAGWAVATTAGNAIAVPAAVALVDPSWQ
                     PYVATATTQVAAAVVVTAILVPLITSWWAKKYGCPKFPKQNEDGTIIA"
     gene            6073..6378
                     /locus_tag="KRL67_002383"
     CDS             6073..6378
                     /locus_tag="KRL67_002383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UxaA family hydrolase"
                     /protein_id="HBH1523257.1"
                     /translation="MINAVIIDEKDNVAVAIEAIAKGSKISFKLKDKTVKTITALDDI
                     TIYHKLAIKDMAQGDKVTKYGEHIGEANQEIKSGQHVHIHNVRSVREDLDKQVMTNI"
     gene            6533..7693
                     /locus_tag="KRL67_002384"
     CDS             6533..7693
                     /locus_tag="KRL67_002384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UxaA family hydrolase"
                     /protein_id="HBH1523258.1"
                     /translation="MKFYGYKRPDGRVGIRNHILILPTSVCASDTTRIIASQVQGAVT
                     FNNQNGCSQVHSDQQLTMDVMAGYAANPNVYGVIVVSLGCENCQNDLVVDAIKERTNK
                     PIKTLVIQEEHGTLKTIEKAVRYAREMAQEASLLRKEEFPISELILGTECGGSDPTSG
                     LAANPLIGELSDKLVDLGATSILSETTEFIGAEHILARRAVNEEVKEKILHIVHRYEN
                     SLKLVGEEVREGNPSPGNIAGGLTSLEEKSLGCIHKGGHRQISEVYDYAKQINKKGLV
                     IMDTPGNDASSVAGMVAGGAQIVVFSTGRGTPSGNPIAPVIKITGNKITFANMEDNMD
                     FDASPVIYGPQTMEELTEDLLNMVVDVANGKQSKAESLGYTEMAIARVCNYV"
     gene            7743..8645
                     /locus_tag="KRL67_002385"
     CDS             7743..8645
                     /locus_tag="KRL67_002385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HBH1523259.1"
                     /translation="MKFVSFTEGENECKRTGVFSKDELFIIDVNSLNLSRNFKDLNEL
                     IQNISSEDIIKLKTIINESTFYNYDVFKRERCTVHVPIEKPIHDILCVGVNYKDHLEE
                     TQTHFDDSFEEPQKTVYFTKRVTKAIGPEDEIDGHFEINNQLDYEVELGVVIGKTGVN
                     IRKEEVEDYIFGYTVINDISARKLQKEHVQWFRGKGLDTFTSLGPCILHKSAVPFPIK
                     LKVCSRVNGEERQSSNTGLFISDVSDIVSELSKGMTLEAGDIIATGTPSGVGMGFSPP
                     RYMSSGDVVECEIESIGILKNYIK"
     gene            8725..9618
                     /locus_tag="KRL67_002386"
     CDS             8725..9618
                     /locus_tag="KRL67_002386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HBH1523260.1"
                     /translation="MKAEFLTPVVTIFDEEGNLDKEGNKKVYDHLIDGGVDGLVIMGS
                     TGEFFNMSMDMQKELIDLVISHVNKRVKVFIGTSRMCINESIELSNYAHNSGADGVMI
                     ISPYYFSLDDDSIELFYSEIAKNTEAKIYLYNYPDRTGYDLSPELTLRLARKHKNIVG
                     YKDTVTLMGHTRDLINTMKIEFPEFKVYSGYDENFIHNLMSGGAGCIGGLSNLIPEVC
                     SKWAEAYNNRDMDKVIEIQKYINKAMDFYLITNPFIPAMKKAMNIRGLQISDYMTAPF
                     VKPNEKQVCEIKKLMNELNIC"
     regulatory      9671..9851
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            10079..11209
                     /locus_tag="KRL67_002387"
     CDS             10079..11209
                     /locus_tag="KRL67_002387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBH1523261.1"
                     /translation="MKFKSNVLKQFFLIAMVIFMVGGLIGCSKNDKENKQASSGKKLE
                     LKYASGFSVEDLEDGIKKVTDGDNRELILIPKKIKIPEKYKNANIVRTPVDNVLIAST
                     TQACSLRTIDELDSIKAVTTEEQYWTIKEIKNLMKDGKIHYVGSNLAPDYEKIVDLNP
                     DLTIVSSGLSEADTKFIDKLKELGFNYVVDNEYKEQNPFGRMEWTSLIAAFYDKEDMA
                     TSELNKTVQKVEEVSKKIKNKAKPKVVWASVYEGSAYIAPKDSYVDNMIKMAGGINAC
                     ASIDSNEGRVSIEQLYEVAKDADIFVYSSSSDYAPNLDSIKSSAPVLADLDVVKNGNL
                     WVFAPDYYQSVDKTDELIVDLVEMFHPGTTGEKIKHYINYDK"
     gene            11281..12306
                     /locus_tag="KRL67_002388"
     CDS             11281..12306
                     /locus_tag="KRL67_002388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HBH1523262.1"
                     /translation="MDFINKMRIRNTTIFIVLIISMILTFLVCVAFGSVKIEFKEVID
                     VVLRGMDNTEHYKIIYNIRIPRVLATMVGGACLAVSGILLQIFFKNPIVEPYILGVSS
                     GSTLSVALMILGGYTLGFESVNSMSIFVAAFIGALIVMSIVILFANKVKNITTLLIIG
                     IMTGYVCSGITNVLQSFANSDKLKEFTMWSMGSFSGFTWEKVEILIIVGMLGLLMTVF
                     IIKPLNAFLLGEEYAKSIGINIRFFRVLIVFISSILAAIVSAFAGPIGFIGLAVPQIS
                     KLVFKTSDNRVLIPVVIFLGSIVTALCDLISRVILSPVELPISTITSLLGAPVVVMLL
                     VKRKDRA"
     gene            12309..13505
                     /locus_tag="KRL67_002389"
     CDS             12309..13505
                     /locus_tag="KRL67_002389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBH1523263.1"
                     /translation="MLKTNNLSVGYNNKVVISNINVEVKNGEILCLLGSNGAGKTTLL
                     RSLSKLISPIKGEIYLNGVNINCISRKALSKKMALVLTNRLLGDLMTVQDIVNIGRYP
                     YTGFFGNLSKKDLIMVDEALESVDALHLKKRYFDELSDGEKQKVLVARALVQEPEIII
                     LDEPTTHLDIKHRLELINILKKLSKEKSISVILSLHEIDIALKSCDKVALIKNNKVIA
                     YGQPEDVVDEDIINSLYELDDKNFNSLLGSVEISNKSKNEVFIIGGGGKATPIYRAFT
                     KKGIGLYSGIIHENDIDYEIGRTMGIKMFTENPFEPISDESFDLAIRNLNDSKIIIDT
                     GFSVGETNKRNIDIVKEALKLDKKVYSFRNRDESKKYYDHLDSKIEHIDKVSQIINSA
                     DINNLL"
     gene            complement(13633..14358)
                     /locus_tag="KRL67_002390"
     CDS             complement(13633..14358)
                     /locus_tag="KRL67_002390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HBH1523264.1"
                     /translation="MKTISKQELEKTLEKHYLWLKNNNAGEKADLKELRLENMDLRGY
                     CLSNIDFSWSDLINLNLEKADLENSIFNNSYLSDCSLKNSILKNADLSGANFRFCDLS
                     DSDIRGANLENSNLEHATLNGVISDESTRFFKLYCPETGAFIGYKKCFNFRMVQLLIP
                     SDAKRVSATSNACRCDKAKVLSITSIDGRESFKSARAYADENFIYIVGETIVAEDFNE
                     NRWKESTTGIHFFLTREEAIGYL"
BASE COUNT         5450 a         1646 c         2496 g         4816 t
ORIGIN      
        1 atagagataa gtctcttttt tactgtttaa atgataaaaa tatatttgat tttataaaag
       61 aagattatct tatgaactaa aaagtttatt ttgaaacaac actacctatt tatgttataa
      121 acttataaat ataatataga atctatgatt ctgtattatt aagtggggtt aagcgttata
      181 attaattata aatattgaat aatctaaatg gataagataa tggtattaaa aattatccta
      241 aaaggtgaaa taatctgtat ttaatgtagt acagttatat atttaaagca aggtggtgaa
      301 atgatataaa taagatatct aaaagagggt ttgatgatag tgataaaaga tggttttcaa
      361 gtaaagaatt aacaagactt ataaaggcta aagaagaaat agaatggcta atcaatagag
      421 attacaaggt agacccagtc gttacttttg ttggtgatag atatcagttt tctataagac
      481 aaagagatgc attaaaaagg gcagtctgta ctgatgaaaa aaatattata agacaatcaa
      541 agaggctttc tttagataaa atcaaagaaa ataccgtaaa tatagatggc tttaatttga
      601 taatatccat agaggtagca ttatctggtg gtactctagt aataggaagt gatggcaata
      661 taagagattt agcaggtctt agaggaactt ataagattat agataaaaca gaagaagctt
      721 tgaatttgat aggtaaattc tttaataaat acaatgcaca gagtcttaaa ttttacctag
      781 attctccagt ttctaattca ggaaatttaa agtatgggat attagagtac gctaaaattt
      841 ggggaattga aactgaagta gaattagtaa aaaatgcaga tgttgtattg gaaaaattag
      901 atagagtagt tagtagtgat gcagttatag ttgataagtg tataagttat tttaatgtgg
      961 ctagaggtat aattgaagag tatattaaag actgcaatat cgtaaattta aataagtaga
     1021 taaatagaaa tgagaatata agtatatata gcaatattat tagtaataca agtaaagttt
     1081 gaaaatagga gaacgaatac atatcgaatt tcataaaatg ttgtaaaaag acatatgaga
     1141 ggatggatat gtgtgaaatt aactaaacta aatattaaaa aatatagagc acctatatat
     1201 aaatatgctt tagcaaatgt aaatttgagg tctgctaaat ctactaattc aagtatcata
     1261 acagtaatac ctcagggagc taaaatggaa gtattggatg aagaagatga ctggattaag
     1321 gtgatgtata attctcaaga aggatatgtg tataaggatt tggtatcagt aagcgaatat
     1381 gcttggagta acttaaactt aagagaaaat aaatctacaa cttccaatat aattacagta
     1441 attcctgaaa aatctagggt tgaagtcctt caagtagatg gagattggag taaggttgta
     1501 tatgatgata aaacaggata tgtttttaat tattttttat caactgatgg aaataaacca
     1561 aatgaattag attataagta tttttatact gacatgatta aatttgttaa cgaaaataat
     1621 attaaaagta ctagtgatta tctaattgta acagacctta gaaataaata tacttatatt
     1681 tttaagaaag ataatggagg ctggggacaa ctttacaagt ggcaatgtac tataggtaag
     1741 cctgagactc caactataac aggtatattt tatataagtg gaagaaaacc ttcttttgga
     1801 acagatgagt attcagttaa gtatgctact agaattaaag gtggatatta ctatcattct
     1861 gtgctatatg attcaacagg aagttatata atagatggca gacttggtga agctcttagc
     1921 catggatgta taagattaag tacggaaaat gcaaagtgga tatacgataa tataccagat
     1981 acaacaacag ttataattca ttaagaaaaa ttttaagttt tttattgaga taatgattct
     2041 taaattaata ggattatata ttaacttctc ataaagagta gtatttaaaa agtgtaataa
     2101 gagagtgtta ttaacaaaat aaaggtattt tgaggcactc tcttataaat atacataatt
     2161 aagatatttt cataaacaat tctataatct tctctttatc aaagaaaaca ggattagcac
     2221 cagcacaagc atctttcata gagttttctg caagcaattc aaaattagga ttatcaacac
     2281 ctacatcttt taaagatttt gggattccaa cctcttcaga aagagctttt atcttatcta
     2341 ttacaaagtc aacacactct ttatctgatt tattatttac atccattcca ataacttcag
     2401 ctattggtcg gaatttggca ggggcagact tagcgttttc ctcctcaact attggaagta
     2461 gcatggcatt acatactcca tgaggtaaat catataaacc tcctaattga tgagccatag
     2521 catgtacatt tccaagacct gcattactaa aggcaattcc atttaaaaaa caagcataac
     2581 acatttgttc tctagcttct atgtcattac catttttaac ggctcttgga agatatttaa
     2641 atatctgctt tatagcatat aaggcagttg aatctgtaac atcataagca ccattagcta
     2701 ctacagcttc tatagcatga gttagtgcat ccataccagt tgctgctgtt aaattagatg
     2761 gtttactaat cattaattct gggtcattta ctgtcattgt ggctagtgaa ttggtatcaa
     2821 ccattatcat ttttacatgt ctgtcttcat cagttataac atagtttatt gtaacttcag
     2881 cagatgtacc agcagtagta gttatagcta taataggaag acatttttta gaagttttgt
     2941 tgataccttc atagtctcta atatctcctc cattagtagc caatatagag atagctttac
     3001 cacagtcttg tggagaacct cctcctatag tgatgacgaa gtcgcaattt tctgacttta
     3061 atattttaag tcctttattc acattagtta cagttggatt ttgtttaatt tcatcataaa
     3121 caacaaagtc tacatctatc tgagataaaa tatctgttac tttttttaca gtaccatttg
     3181 aaactagaaa tttatcacta acaacaaatg cttttttagt tcctaatgat ttaattgaat
     3241 ctttagcttc attaagacat cctttcccaa gtaaatttat tggtggtaca taataaatgc
     3301 tcataactaa aatctccttt tattttcatt aatttttatg taaaaatata tatctaacaa
     3361 ttttcactaa tttactcata atatatcgta tcttagattg atatagagtc agtagttctt
     3421 atataagaac ttaattcgta attacgatta tgattagaat ttaacatagt atttttatca
     3481 tgtcaataaa aatataaaaa tattgtaaaa aaaaacaaag ttactgtagt taaatcagca
     3541 actttgtttt tatagtataa atcaaataaa tacattagtt taaaacaaaa tttttaatta
     3601 ttatgttttg atatcttcca tatacagaat attaatctaa tttattacaa tgtcttcatt
     3661 acgttcttcc atcattgatt gaatattttt ctgagcattt aagagtaatt tttttatcag
     3721 ttctattttt tcatcattag ctctaaataa aggcagtgtt acactaacag cagctcttat
     3781 acatccattt tttcttattg gtacggcaag acattttagg tgttccattg attcctcaca
     3841 ctctgaagct atttgtgttc tttgaacttc aagaagctgg tcgtataaaa catcaaaatc
     3901 tgtaattgat ttttctgtaa aaccttttag accatctgga tataagctta ttagtttttc
     3961 cttgtcaaaa tcacataaaa gagcttttcc aagacctgta caattagcag gtattttttt
     4021 tccaacataa gaaataagtc taattggttc tggagaatct acttttccaa tataaagaac
     4081 ttcattattg tcaagtatac caagctgaca agtttcagaa caggaattaa ctatattcgt
     4141 catttcgtca attatatagt taaaaatagt tcttttttca aaaaatgaag accctaatgt
     4201 gtaagaacgt atccctatag aatatttgga tgtgttttta tcaatagtaa tgtattttct
     4261 agcattaagt gtatgaacta taggaaagat gctacttttt ggagcattga tagcatcggc
     4321 tatttctgta agagtatatc cattttcgga ttctgctaat gcttctaaaa tatctaatat
     4381 cctagctgtt gggcgatgat tatctgaagt cataaatttc cccctctttt tattaattta
     4441 aagtattaga atatgttgat tttagcacac tatacgatat tttacaatgt agaaaataaa
     4501 tatgtaaatt tatatataaa taagctcttg atttagatac agatagatat ttttttgggc
     4561 tgtacattaa ggaatagcta atttccaaaa tatacctaaa gaaagtaagt gatatttagt
     4621 tatataattt aagtaaatat atgaaatagt gatttttata tgtaaatatc aaaatattat
     4681 gaatttatat tttataattt atctattttt acacggtata aaaactttat agttttgaat
     4741 actatataat tttaaatacg ttgtattttt gcatataagg aagccatttg ataatataca
     4801 atgtattatc agaaagtttg aaaaaatttt gacaaaaatt tatttttttt attgactcat
     4861 aaataggaaa atgctatcat taattcaaag attcgtaatt aaaaacctag ttcttaaata
     4921 cgaactaaaa cggccgttag aattaaagct aaaaacttaa taagagtaat taaatatcat
     4981 gttttaggag gattaacttt atgataatgg attttattaa aaaaattcct gcaggtatga
     5041 tgatagtacc aatgtttata gcagctttca ttaatacctt ttgcccagat attgtacaaa
     5101 taggttcgtt tactacagca gtattttcaa gtgttgggtc tgcaactata attggggttc
     5161 aactggtatg cttgggaact cagcttcaat ttagagaaat gccaaaagtt ttaaaaagag
     5221 gtggtatact tcttggttct aaatttgcta taggtgcttt gataggtata cttataggaa
     5281 agttatttgg aatggatggt gtccttggtc ttacaacatt ggctgttata agtgcagtta
     5341 caaatagtaa tggtagtata tatctttcat tgatgacttc atatggagat gaaacagatt
     5401 gtgcaactat gagtttactt actttaaatg atggaccatt ttttacatta atagctcttg
     5461 ggacttcagg tcttgcaaat gtacctttgt tgtcattatt ggctgcaata gtgccaatac
     5521 ttgttggtat gataatagga aattttgaca aaaaaatgaa agagttttta gagccagggt
     5581 gtaatttact cgtacctttt gtagggtttg cacttggtag tagcataaat cttgcaagta
     5641 tactaaaagg aggtttatca ggagtattac ttggattaat atctgtattt gtaggaggta
     5701 tatttatagt attctgtgat aaatttatag gaaaacgtcc aggatatgca ggatgggctg
     5761 tagctacaac tgctggaaat gcaattgctg tacctgctgc tgttgcatta gttgacccat
     5821 catggcaacc atatgttgca acagctacaa cacaagtggc agccgcagta gttgtcactg
     5881 caatattagt acctcttata actagttggt gggctaagaa gtatggatgt cctaagtttc
     5941 caaaacaaaa tgaggatggt acaataatag cttaatttat attacagtga gttgttggta
     6001 aaataataaa ttctttaatt agattttagg atattaataa aattatatta tttataaagg
     6061 agagcaagaa taatgataaa tgcagtgata attgatgaaa aagataatgt tgcagtagct
     6121 attgaagcaa tagctaaagg tagtaaaatt agttttaaat taaaagacaa aacagtaaaa
     6181 acgattacag cactagatga tataactatt tatcataaat tggctataaa agatatggcc
     6241 caaggtgata aagttactaa atatggtgaa catataggag aagcaaatca ggaaataaaa
     6301 agtggtcaac atgtacatat tcataatgtt agaagtgtta gagaggattt ggataaacaa
     6361 gttatgacta atatataaaa taatatttat agagttataa aagttgtatt cattgctttt
     6421 agtgtcaaat agtttttaaa taggatttta gctgtttgat tagtgagtgt aataaaaatt
     6481 ataatatatg aaaattataa aagtatatag actattttag gaggaaatca gtatgaaatt
     6541 ttatggatat aaaagaccag atggaagagt tggtataaga aaccacatat taatattgcc
     6601 tacgagtgtg tgtgcatcag atacaactag aataatagca tcacaagttc aaggagcagt
     6661 tacctttaat aatcagaatg gatgttcaca agtacattca gaccaacaat taactatgga
     6721 tgttatggct ggttatgcag caaatccaaa tgtatatggt gtaatagttg tatctttggg
     6781 atgcgaaaat tgccaaaatg atttggtagt tgatgcaata aaagagagaa caaataagcc
     6841 aataaaaact cttgtaatac aagaagaaca tggaacttta aaaactatag aaaaagctgt
     6901 tcgttatgct agagaaatgg cacaagaagc ttctttactt agaaaagaag aatttccaat
     6961 atctgaactt atactaggaa ctgaatgtgg tgggtctgac ccaacaagtg gattagcagc
     7021 aaatccatta ataggtgaat taagtgataa acttgtcgat ttaggagcaa catcaatttt
     7081 aagtgaaact actgaattta taggtgcaga gcatatacta gctaggagag cagtaaatga
     7141 agaagtaaaa gaaaaaatat tacatatagt acatagatat gaaaattcat taaaattggt
     7201 tggagaagaa gttagagaag gtaatccatc tcctggaaat atagctggtg gtttaacaag
     7261 tttagaagaa aaatcacttg gttgtataca taaaggtggt catagacaaa taagtgaagt
     7321 atatgattat gccaaacaaa ttaataagaa aggtcttgtt ataatggata cccctggaaa
     7381 tgatgcttca tctgttgctg gtatggtagc aggaggagca caaatagtgg tatttagtac
     7441 aggaagagga acaccatcag gaaatccaat agctccagta ataaaaataa ctggaaataa
     7501 aataactttt gcaaatatgg aagataatat ggattttgat gctagtccag ttatatatgg
     7561 tccacaaaca atggaagaat taacagagga tttgttaaat atggttgtag atgtagcaaa
     7621 tggaaaacaa tcaaaagcag agtctcttgg atatactgaa atggcaatag ctagagtatg
     7681 caattatgta taatctttaa gtaagaaaat aaatacatag aattttaata aagggtgaga
     7741 ttatgaagtt tgtaagtttt actgaaggag aaaatgaatg taaaagaact ggagtttttt
     7801 ctaaagatga attatttatt atagatgtta actctttaaa tcttagtaga aattttaagg
     7861 atttaaatga attaattcaa aatatatcta gcgaagatat tattaagttg aaaactatta
     7921 taaatgaaag tacattctat aattatgatg tttttaaaag agagagatgt actgtacatg
     7981 ttcctataga aaaaccaata catgatatct tatgtgtggg agtgaactat aaagaccatc
     8041 ttgaagaaac tcaaactcac tttgatgata gttttgaaga accacaaaaa acggtttatt
     8101 ttacaaaacg tgtgacaaag gcaataggtc cagaagatga gatagatggt cattttgaga
     8161 taaataatca actagactat gaagttgaac ttggagtagt aataggtaaa acaggtgtta
     8221 atattagaaa agaagaggta gaagattata tctttggtta cactgttata aatgatattt
     8281 ctgctagaaa gttgcaaaaa gaacatgttc aatggtttag aggaaaagga ttagatacat
     8341 ttacctcttt aggtccatgt attttacata aaagtgcagt tccttttccc ataaaactta
     8401 aagtatgtag tagggtaaat ggtgaagaaa gacaaagttc taatacagga ttattcatat
     8461 cagacgtttc agacatagtt tcagaacttt caaaaggtat gactttagag gctggagaca
     8521 ttatagctac tggaacacct tctggagtag ggatgggatt ttcacctcct cgatatatga
     8581 gtagtggaga tgtagttgaa tgtgagattg agagtattgg aattttaaaa aattacatta
     8641 agtaaatgtt ttaatataat taatattaaa gctaaatctg atgtattaat gaacatatta
     8701 aaataaattt taggagttta aattatgaaa gcagaatttt tgacaccagt tgttacaata
     8761 tttgatgaag aaggaaattt agataaagaa ggtaataaaa aagtgtacga ccatcttata
     8821 gatggaggtg tagatggact agtcataatg ggaagtactg gagagttctt taatatgtct
     8881 atggatatgc aaaaagaact tatagattta gtaattagtc atgtaaataa gagagtaaaa
     8941 gtgtttatag gtacaagtcg tatgtgtata aatgaatcta tagaactttc aaactatgca
     9001 cataatagtg gagcagatgg agttatgata ataagccctt attatttctc tcttgatgat
     9061 gatagtattg agttatttta tagtgaaata gctaagaata cagaggctaa aatatattta
     9121 tacaattatc cagatagaac tggttatgat ttaagtccag aacttacatt aagacttgca
     9181 agaaaacata aaaacatagt tggatataag gatacagtaa ctttgatggg acataccaga
     9241 gatttaataa atactatgaa aatagagttc ccagaattta aggtttatag tggttatgat
     9301 gaaaacttta tacataatct aatgtctggg ggagcaggtt gtataggggg actatcaaat
     9361 ttaattccag aagtttgctc caaatgggct gaagcttaca ataatagaga tatggacaag
     9421 gttatagaaa ttcaaaaata tattaataaa gcaatggatt tttacttaat aaccaatcca
     9481 tttataccag caatgaaaaa agctatgaat atcagaggac tacaaataag cgattatatg
     9541 actgctccat ttgtaaaacc aaatgaaaaa caagtatgtg aaattaaaaa attgatgaat
     9601 gaattaaata tatgttaaat caaccttgca aagaggtcaa caataaatta caattatctt
     9661 aataaaaatt aaattaaaat aatactaggg tactatatga gtttaatagg gaaagtgatg
     9721 aaaattcact acagcccccg ctactgtata cggatacgaa atcaaaatcc actgaaatta
     9781 taaaaatttt gggaaggatg atggaggaaa agccgtgagt caggagacct gcccagtatt
     9841 atataagttc aatttagggt ggagattgta atttataata aaatgaagta tcaattcgtt
     9901 tttgtaaagt tgatatatga acttctcctt attaaattag gagaagtttt ttattttatt
     9961 aaaaggttat acttattaaa gatgacttta ggataacttt aataagtata ctttaaaaag
    10021 catatattat taaagttttt tagccactca taaaaaataa gatattggag tgaaagagat
    10081 gaaatttaaa tcaaatgttt taaagcagtt ttttttaatt gctatggtaa tttttatggt
    10141 aggaggtctt atagggtgta gtaaaaatga caaggagaat aaacaagcta gtagtggtaa
    10201 gaaattagaa ttaaaatatg ctagtggatt tagtgttgaa gatttagaag atggaattaa
    10261 aaaagtgact gatggagata atcgagaact gattttaatt ccaaagaaga ttaaaattcc
    10321 tgaaaaatat aaaaatgcta atatagttag aacgcctgtg gacaatgtat taatagcatc
    10381 tacaacacag gcttgttctc tgagaacaat agatgaacta gatagtataa aagcagtaac
    10441 aacagaagag cagtactgga ctattaaaga aattaaaaat ttaatgaaag atggaaaaat
    10501 acattacgtt ggttcaaatt tagcaccaga ttatgaaaaa atagtagatt taaatcctga
    10561 cttaactata gtgagtagtg gcttatcaga agctgataca aaatttattg ataagttaaa
    10621 ggaattaggg tttaattatg ttgtagataa tgaatataaa gagcaaaatc catttggacg
    10681 aatggagtgg acaagtttaa tagctgcatt ttatgataaa gaagatatgg ctactagtga
    10741 actaaataaa actgtacaaa aggtagaaga agtaagtaaa aaaataaaaa acaaagctaa
    10801 acctaaagtt gtatgggcat ctgtatatga aggaagtgct tatatagctc ctaaagattc
    10861 atatgtagat aatatgataa aaatggctgg tggaataaat gcatgtgctt caatagattc
    10921 aaatgaaggg cgagtaagta ttgaacaatt gtatgaagtt gcaaaagatg cagatatatt
    10981 tgtatactca tcaagttcag attatgcacc aaatttagat tctataaaat ctagtgcacc
    11041 tgtattggct gatttggatg ttgtaaaaaa cggaaaccta tgggtgtttg ctccagatta
    11101 ttatcaatca gtagataaga cggatgaatt aatagtagat ttagttgaga tgttccaccc
    11161 aggtactaca ggtgaaaaaa tcaaacatta tataaattat gataaatagt acagagagga
    11221 cacaagaaga taggttttat agattttagt taatatagct ttgctaagag gagtatacaa
    11281 atggatttta tcaataaaat gagaataagg aatacgacaa tatttatagt attaattata
    11341 agtatgatac ttactttttt agtgtgtgta gcatttggct ctgtaaaaat tgaatttaaa
    11401 gaagtaatag atgttgtatt aagaggtatg gataatacag aacattataa aataatatac
    11461 aatataagaa tccctagagt acttgcaacc atggtaggtg gagcatgcct agcagtatca
    11521 ggtattttat tacagatatt ttttaaaaac ccaatagttg aaccgtatat attaggagta
    11581 tcttcaggtt ctactttatc agtagctctc atgattttgg gaggatacac attgggattt
    11641 gaaagtgtaa attcaatgag tatatttgta gcagcattta taggagcttt aattgtaatg
    11701 tctatagtca ttttatttgc aaataaggta aagaatataa caactctttt aataatagga
    11761 attatgactg gatatgtatg tagtggcata acaaatgtgt tacaatcatt tgcaaatagt
    11821 gataaattaa aagaatttac tatgtggtct atgggaagtt tttctggatt tacatgggaa
    11881 aaggtagaaa tacttataat agttggtatg ttaggtcttt tgatgactgt attcataata
    11941 aaacctttaa atgctttttt attaggagaa gagtatgcaa aaagtattgg cataaatata
    12001 agatttttta gagttttgat agtatttata tctagtatat tggcagcaat agtatcagca
    12061 tttgcaggac ctatagggtt tattggactt gcagtgccac aaatttcaaa attggtattt
    12121 aaaacatcag acaatagagt tttgatacca gtagtaatat ttttaggcag tatagttaca
    12181 gctctttgtg atttaatatc aagagtaatt ttatcaccag tagaattacc aataagtacg
    12241 ataacatctt tattaggagc tccagttgta gtaatgttat tggtgaaaag gaaggatagg
    12301 gcttagacat gttgaaaact aataatttaa gtgtaggata caataacaag gtagttataa
    12361 gtaatataaa tgtagaagta aaaaatggag aaatactatg tcttttggga agtaatggag
    12421 ctggaaaaac taccctatta aggtccttat ctaaacttat atcaccaata aaaggtgaaa
    12481 tatatttgaa tggtgtaaac ataaattgta tatcaagaaa agcattatca aaaaaaatgg
    12541 ctttagtact tacaaataga ttattaggtg atttaatgac tgtacaagat atagtcaata
    12601 taggaaggta tccatataca ggattttttg ggaatctatc taagaaagat ttaataatgg
    12661 tagatgaagc attggagtct gtagatgcct tacatttaaa gaaaagatat tttgatgaac
    12721 taagtgatgg agaaaaacaa aaggtgttgg ttgcaagagc tctcgttcaa gaaccagaaa
    12781 taattatatt agatgagcct acaactcact tggatataaa acatagattg gaacttataa
    12841 acatactgaa aaaattaagt aaagaaaagt caatatcagt aatactttca ttacatgaaa
    12901 tagacatagc attaaaaagt tgtgataagg ttgctcttat aaaaaacaat aaagtcatag
    12961 cttatggtca acctgaagat gtagtagatg aggatattat aaattcactt tatgaattgg
    13021 acgataaaaa ttttaatagt cttttgggtt cagtagaaat tagtaataaa tctaaaaatg
    13081 aagtttttat aattggtggt gggggtaaag caacaccaat ttatagagct tttactaaaa
    13141 aaggtatagg tctgtattct gggataatac atgaaaatga tattgattat gaaattggta
    13201 gaactatggg gataaagatg tttacagaaa atccatttga gcctataagt gatgagagtt
    13261 tcgatttggc tattagaaat ttaaatgatt ctaagattat aatagataca ggatttagtg
    13321 taggagaaac aaacaagagg aatatagata ttgtaaaaga ggctttaaaa ttggataaaa
    13381 aagtttattc ttttagaaat agagatgaga gcaagaagta ttatgaccac ttggatagca
    13441 agatagaaca tatagataaa gtttctcaaa taattaatag tgcagatata aataatttat
    13501 tataatttat gaaataaaat gtacttatgt gctatagaaa aaagagtata gattataatt
    13561 tttttgaaat tacaatccat actcttttgt tattttgttt atgtatttat atatttataa
    13621 aactttagca ttttatagat aaccaatagc ttcctctcta gttaaaaaaa agtgaatccc
    13681 tgtagtcgat tctttccatc tattttcatt aaaatcttca gctacaatcg tttctcctac
    13741 tatatatata aaattttcat cagcatatgc acgagcactt ttaaaagatt ctctaccatc
    13801 tatactagta attgacagaa cttttgcctt atcacatcta caagcatttg atgtagcaga
    13861 cactcttttt gcgtcactgg gtattaataa ttgtaccatt ctaaaattaa aacatttttt
    13921 atagcctata aatgctcctg tttcaggaca atacaattta aaaaaacgag tactttcatc
    13981 tgatattact ccatttaaag tagcatgttc taaattggaa ttttctaaat tagctcctct
    14041 aatatctgag tcagataagt cacaaaatct aaaatttgca ccacttaagt ctgcattttt
    14101 taatatagaa ttttttaatg aacaatccga aagataagag ttattaaaaa tagaattttc
    14161 taaatctgct ttttctaaat ttaaattgat aaggtcagac cacgaaaagt caatattact
    14221 taagcagtat cctcttaagt ccatattttc taatcttaat tcttttaaat ctgccttttc
    14281 tccagcatta ttgtttttaa gccataaata atgtttctca agtgtttttt ctaattcttg
    14341 tttagaaata gttttcatta aaatcacctt cataaattta tttttatata tgtagtagca
    14401 aaatagtc
//
LOCUS       DAEPXF010000092        14402 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain HB27
            SAMN13376389-rid12479423.denovo.097, whole genome shotgun sequence.
ACCESSION   DAEPXF010000092 DAEPXF010000000
VERSION     DAEPXF010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13376389
            Sequence Read Archive: SRR10518432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 14402)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14402)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001069624.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001069624.1
            Genome Coverage       :: 315x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/15/2021 01:54:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,165
            CDSs (total)                      :: 4,076
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 89
            rRNAs                             :: 10, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 10 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 19 of 69
            Pseudo Genes (incomplete)         :: 44 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 10 of 69
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14402
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13376389-rid12479423.denovo.097"
                     /strain="HB27"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="China: Hebei"
                     /collection_date="2017"
     gene            312..2399
                     /locus_tag="KRL67_002391"
     CDS             312..2399
                     /locus_tag="KRL67_002391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011862017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HBH1523265.1"
                     /translation="MVVDKEIVSILQILLNSTLITTNGLQEESNSSKRQITYRINKIN
                     DMLKVKNVPLICLRADKDIIVRKETKKAIKEILEKNYSKNTYYFSKTERLLYMYLMLF
                     INMEDISINHFINSIKVSRSTVNLDFKDLIPELEKKNIKVKNNRINGYYIVGNEMEIR
                     RVLIKNIIETLSNEKSSRVFDIFIKEYNLDSFEEAKKIILKLIKKYEITFVEDRLVEF
                     IYIFIFLKARMYSTRIVTQENVDIPNISVMSSMKEYKFAKELLEVYGSEDKIKPHNIM
                     YISAWILGISVGNYEEDTEDKDVISKIVNKMMTKFGYLSGVYYISQEKIFKQLYSHFR
                     PAYYRLLFKLPIYNPICEKVKEEYKLVYRIVSNAMKEFCGLFGEEIQEEELAYLTMHF
                     ATLFSNKKEFDGFRKRIALIVCSNGVGSSAILHAELTNLFPDLHFIPMSESVGLENIS
                     EPVDIVFTTNYNAEGLKTDVPVIKVSPVMTTKEKYKITREVYIQLGDIYLRQPKIDEV
                     MDIIKKYANVTSESILSSELLAYFTQIENFTSKEGEGPMLSEITNETLVKIKVKAKNW
                     EEAIRQSASVLVENNKVTEGYVDAMINSAKASGPYIVITKHVALPHARPEAGSKEIAI
                     GIATLDSPIEFGNAENDPVKYVFCLSAIDNNSHLRAMSELVELLEEDEFFKVLDSAKE
                     AKEIIDYIKTHEL"
     gene            2414..2701
                     /locus_tag="KRL67_002392"
     CDS             2414..2701
                     /locus_tag="KRL67_002392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBH1523266.1"
                     /translation="MYKILIACRAGVGSSLMLKIKTQQIIKENNFPIEVEHGSLDSLN
                     GFNGDAVETLIDVAEELKNKNLKFDIIGIHNIVDRNEIKTELEKFLASKEG"
     gene            2728..3999
                     /locus_tag="KRL67_002393"
     CDS             2728..3999
                     /locus_tag="KRL67_002393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIC"
                     /protein_id="HBH1523267.1"
                     /translation="MEFIISLLSTPAVLLGVVACIGLILQKKSAIEVFTGSSKTLIGF
                     LIFGIGASAMTGALQNFNKLFQHGFGITGVIASPEAATALAQGTYGFAVSCTLILGFI
                     LNLVFARITKFKNIFFTTGHSLFFSCVLVLIMKAHGFDNTITILIGGTILGFMSAALP
                     QFCQPFMRELTGGDEQAIGHFNMIGYGLSGYIGRLFSKHKDDTTETIEFPKWLSIFRD
                     FLMGLSIVMLILFYIATLKAGKVFTQEIAGSTHWLVFPLIEAFTFVAGMSILMSGVRM
                     FLAEITAAFVIISEKYIPGSRPALDVPTVFPYAPNAVIIGFISAYAAGLLAIAIMASF
                     PSIFPVVIIPAAHICFFSGGTAAIFGNTSGGWRGAIAGSFVVGLLLAFLPVILYPVYG
                     TLGIEGATFPNIDYNVVGSILHNILQFIKPI"
     gene            4057..4656
                     /locus_tag="KRL67_002394"
     CDS             4057..4656
                     /locus_tag="KRL67_002394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HBH1523268.1"
                     /translation="MENTLSEFLDNISNELSEFIASIDETSINKACELILEAEKNHGR
                     VHVTGIGKPGHVSGYISSLLSSTGTSAYILHGTEAVHGSSGQVVEGDVVIAISNSGET
                     QELKATLKTLKVNGAKIIGVSGNESSFLKNISDIFLFAGVKQEGDCLNKAPRASILAE
                     TIILQSLSVVLQYAKGLNTQQYLKWHPAGSLGKSIREGI"
     gene            4661..5566
                     /gene="dapA"
                     /locus_tag="KRL67_002395"
     CDS             4661..5566
                     /gene="dapA"
                     /locus_tag="KRL67_002395"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HBH1523269.1"
                     /translation="MKYKGIICAMITPFDENQNINSQATCQLIDYLIEKGIYGLFILG
                     TNGECHVLTDDEKVEFAKIVINHTNNRVPVFVGTGGNSTREVVDLSKRMEAIGASALS
                     IITPCFVTPTQQELILHYKTIANSVNLPIIMYNMPGKTGINIEPESVRELSKIKNIVG
                     IKDSSGKLDNMKAYIEATKNENFSVFSGSDSLILDSLKAGSQGAVAATTNFLTEIGIS
                     IYENFMKGDLEEAQKAQSSIEELRRILKLGTIPSVMKKTIVLNGINVGTARLPVTEPT
                     GEVLEEIKRVVENYRTILNKSSENR"
     gene            5672..6304
                     /locus_tag="KRL67_002396"
     CDS             5672..6304
                     /locus_tag="KRL67_002396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketohydroxyglutarate aldolase"
                     /protein_id="HBH1523270.1"
                     /translation="MKKMNITKRMSECGALAIVREENLNRACEIAEGCIKGGITVIEM
                     SYTINNAGEIIQGLNKKYGEILCVGAGTVLDSETARHAVLHGAQFIIAPNYNEGVAKI
                     CNRYQIPYAPGCTSLTEAVDALSLGATFIKAFPISDFYGPKLVKVFKTPIPDMPVLAS
                     GGINIENLHIWLENGVDVCGFGGLLTKGTVEDITENAKKIREIIKNIREV"
     gene            6718..8880
                     /locus_tag="KRL67_002397"
     CDS             6718..8880
                     /locus_tag="KRL67_002397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1430 domain-containing protein"
                     /protein_id="HBH1523271.1"
                     /translation="MKKIIYVLFTIELLIVSLLGLNIVKDNEINNILYSDTTSISVMF
                     KDYKKLNKNYSKWIKDIADENNVAISKYVFNNNEKLSIYTTDTSLSNNIRLKSGKFPN
                     INSNEFISNINTNNSNQVGKFSTMSKDISILIRDFGKISEVGESGILYISTQNEDTIN
                     KILKELNVDRSIFVARLHDRYVNSNLYLNPSIIRDLILIILCFLATIIHYSISVSREA
                     SILRLNGYSKLDIIARVIKNILRIMILSSVTALLIYIIYILKLNLGVRTCLYFVMFSI
                     ICILINILVSVLIVGFNSRSSKYVLSLNGKKSYGFVNIMHFTLKIVFVSFLILSINNC
                     ILNYNMLQTQLGNLSEWDKAKNVYNLTLKDTGEQSLGKEEVIKNAKIKSFYKSLVEEK
                     DAFLIDTTNFEKLEDGSYMYEINSKGKNPEVSQYGKNIKINKNYLKVNPIYSNGKEIF
                     NLIDYNDKTINLLVPKKLQVHENDIKKEFRELFYFEKIEVENMYNEKLNRGLNKTKES
                     DLNVNIIYVDNNQSYFTYNSFVMEDNRNLIDDPIVIIDIDNIDDSFYLSYISRCVYFN
                     SKKLDALADISNIIESQGVEAYIQSLHAVYNEYGLEINKLEKYLNSEIFTIIIIAISN
                     LMITYNIVASYYERNKYKLYIKKIFGYSTTARSMLLIVSLILTNIIPISIISTRVDLS
                     NNIILFGFLILVVEVIISIILDKIISNSSFNKIIKGEH"
     gene            8885..9502
                     /locus_tag="KRL67_002398"
     CDS             8885..9502
                     /locus_tag="KRL67_002398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBH1523272.1"
                     /translation="MISLINISKKFKDKSIIDKFNLEISKGEFVAIIGNSGSGKTTLL
                     NIMGLLEKPNSGDIIINNIKNPNSKQIRILQRDFYGYLFQNYALIENENVENNLKIAL
                     KYQKNINHNEKINYALESVSLRKYNKKKIYELSGGEQQRVALARVILKECEVIFADEP
                     TGNLDKKNRDIVFDILKSLNKNGKTIVFVTHDLELSEQADRVIKL"
     gene            9691..10035
                     /locus_tag="KRL67_002399"
     CDS             9691..10035
                     /locus_tag="KRL67_002399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000212737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkylphosphonate utilization protein"
                     /protein_id="HBH1523273.1"
                     /translation="MENLPNCPKCNSEYTYEDGTLLVCPECSHEWALGLGAEEDSNII
                     KDANGNVLNDGDSVTVIRDLKVKGSSSSIKIGTKVKNIRLIHDSSDGHDIECKIDGFG
                     AMKLKSSVVKKA"
     gene            10605..11471
                     /locus_tag="KRL67_002400"
     CDS             10605..11471
                     /locus_tag="KRL67_002400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HBH1523274.1"
                     /translation="MIDIHKNIYDNKLFEELKIDCKKCFGLCCVALYFSASDGFPIDK
                     ESGKPCSNLQPDFKCSVHNSLMKRGFKGCTAYDCFGSGQKVAQVTYKGIDWMQSPELT
                     NQMSEVFLIMRQLHEMLWYLKEASVLNISDTIKSKIDLIIEETEKITNMSPEQIIDLD
                     IISHRTKVNLLLSQASESVMGKVKSFIKTSTLKNMKKLSKNIDLIGADLRKTNLIGAD
                     LRGRFLIAANLRNTDLSGANLIGADLRDCDIRGANLENSIFLTQLQVNTAKGDSSTKL
                     PASLIRPKYWEK"
     gene            11584..11988
                     /locus_tag="KRL67_002401"
     CDS             11584..11988
                     /locus_tag="KRL67_002401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmaF family protein"
                     /protein_id="HBH1523275.1"
                     /translation="MSYQNYKCERDYVYDDYKYNKCNKCYNDYEEMTHVHEYSESVKL
                     AEECEDRHNHRAAGVTGEVIPINGGTNHVHKINDNVDFLDHFHKICVTTGPAIRIPGT
                     DKHIHLICGETTVNDGHCHKFLFTTQIEAPLV"
     gene            12164..12613
                     /locus_tag="KRL67_002402"
     CDS             12164..12613
                     /locus_tag="KRL67_002402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011862010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBH1523276.1"
                     /translation="MKSEIILEMKQINSTEYDEMLQVWESSVRATHDFLTKKDIESLI
                     PLVEIGLKEVENIVCIKDNDIIKGFIGIDKDKIEMLFIEDKYRGNGIGKKLIKYAIDK
                     YNVKYVDVNEQNKKAVGFYIHLGFKAFDRSEIDGQGNPFPILHMKLI"
     gene            complement(12770..13147)
                     /locus_tag="KRL67_002403"
     CDS             complement(12770..13147)
                     /locus_tag="KRL67_002403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HBH1523277.1"
                     /translation="MLTPYLIFNGTCEKAFNFYAEAFGGGKTIFARLDSNPNNPVMHA
                     SVTFTKYEGCIMGADTDKPVVISGMAICVVLPSREAIEEISVKLAEGGTLVQEFLPHP
                     PPHQNDGAAEVLDRYGYTWYLST"
     gene            13409..14326
                     /locus_tag="KRL67_002404"
     CDS             13409..14326
                     /locus_tag="KRL67_002404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011862008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2268 domain-containing protein"
                     /protein_id="HBH1523278.1"
                     /translation="MVNMNIIEIRSDKIYKKIMDSPINKKEDIYRYELMKPFEFKWKC
                     MNVPIVAKQKGGYDVIIASEMLGVLSPKDIDEKQKKNINVLSADKIWNTCKETIENSI
                     NAFIKEGYDLNIKDYKYSILLANPNSSYTILSDGYWGDGGIPGYIFLSLVPNEYTINR
                     LPVLIAHECNHNIRFQFIEWNNNITLEEMMINEGLAENFATWMFGDEMVGPWVSRIDI
                     ETLNTYIKPIIKSALKETGFQNITSYLYGDDIAKMQGYFPVGLPYCAGYACGYYMIKY
                     YLEKTNKSIIEATLLPYSEIIEAVKEFWE"
BASE COUNT         5619 a         1549 c         2414 g         4820 t
ORIGIN      
        1 acatcaattt tattttaaat taatgtgctt tttaattgta gtaatttatt tataatcaat
       61 atgattttta ttatttaaat caatttttct tgagtttctt tgtctaaaga aacttttttt
      121 atttataaaa tctacagtta tattatagta tctattttag agaagtttaa tttttgtatc
      181 aatctaaaac aaaagttata cttaaatgaa attgaaaacg ataaaattct caaataaaat
      241 aagtattgtg gaggtgatga aagaatccaa tgataatatg aagactaatc ttaataagaa
      301 agtaggggtt aatggtagtg gacaaagaaa ttgtatcaat tctccagatt ttattaaata
      361 gcacattgat tacaactaat ggattacaag aggagtctaa ttcatcaaag agacaaatta
      421 cttatagaat aaataaaatc aatgacatgc ttaaagtaaa aaatgtacca cttatatgtt
      481 taagagctga taaagatatt atcgttagaa aagaaacaaa aaaagcaatt aaagaaattt
      541 tagaaaaaaa ctattctaag aatacttatt attttagtaa gacagaaaga cttttatata
      601 tgtatttaat gctttttatt aacatggagg atatttcaat taatcatttt ataaattcta
      661 taaaagtaag taggtctact gtaaatcttg atttcaagga tttgatacca gagcttgaaa
      721 agaagaacat taaagttaag aacaatcgta taaatggata ttacatagtt ggcaatgaga
      781 tggaaattag gagagttctt attaaaaaca ttatagaaac tttgtctaat gagaaaagtt
      841 caagagtatt tgatattttt attaaagaat ataatttaga tagttttgaa gaggctaaaa
      901 aaattatatt aaagctaata aaaaaatatg aaattacttt tgttgaagat agattagtag
      961 aatttatata tatatttatc tttctaaagg caaggatgta ttcaactaga atagttactc
     1021 aggaaaatgt agatattcct aatatttcag ttatgagttc aatgaaggag tataaatttg
     1081 ctaaggaatt attagaagta tatgggagtg aagataagat aaagcctcat aatataatgt
     1141 atattagtgc ttggatttta ggtatatcag taggaaatta tgaagaagat acagaagata
     1201 aggatgtaat ttcaaaaatt gtaaataaaa tgatgacaaa gtttggatat ttaagcggag
     1261 tttactatat aagccaagaa aaaatattta aacaattata ttctcatttt agacctgcat
     1321 actatagact tttatttaaa ttacctattt ataatccgat ttgtgaaaaa gtaaaagaag
     1381 aatataaatt agtttataga attgtaagta atgcaatgaa agagttttgc ggattatttg
     1441 gagaagaaat tcaggaagaa gagttagcat atcttacaat gcactttgca acactttttt
     1501 caaataaaaa agaatttgat ggttttagaa aaagaatagc attaatagtt tgttcaaatg
     1561 gcgttggtag ttcagctatt ttacatgcag aacttacaaa cttatttcct gacttacact
     1621 ttataccaat gtcggaatca gttggattag aaaatataag tgaacctgta gatattgtat
     1681 ttactactaa ttataacgca gagggtttaa aaacagatgt accagtaatt aaagtaagcc
     1741 ctgttatgac cacaaaagaa aaatataaga ttacacgtga agtgtatatt caattaggtg
     1801 atatttattt aagacaacct aaaatagatg aggttatgga tataataaaa aaatatgcaa
     1861 atgtaacctc tgaaagtatt ctttcaagtg agctattagc ttattttaca cagatagaga
     1921 attttacatc taaggaagga gaggggccta tgctaagtga gattactaat gaaacactag
     1981 taaaaataaa ggtaaaagct aagaattggg aggaagctat tagacagtca gcttctgtat
     2041 tagttgaaaa taacaaagta actgaagggt atgtagatgc tatgattaac tcagcaaaag
     2101 catctggacc atacatagta attacaaaac atgttgcact tccacatgct agaccagaag
     2161 ctgggtcaaa agagattgca ataggcattg ctactttaga tagtcctatt gagtttggga
     2221 atgctgaaaa tgaccctgta aaatatgtat tttgtctaag tgcaatagac aataatagcc
     2281 atttacgtgc tatgtctgag ctagttgaac tattggaaga agatgagttt tttaaggttt
     2341 tagatagtgc taaagaggct aaagaaatta ttgattatat aaaaacacac gaattataaa
     2401 ggaggattta attatgtata aaattttaat cgcatgtaga gcaggagttg gttcaagttt
     2461 aatgttaaaa ataaagactc aacaaattat taaagaaaat aactttccaa ttgaagtaga
     2521 acatggttca ttggattcac taaatgggtt caatggagat gctgtagaaa cattaattga
     2581 tgttgctgaa gaattaaaaa ataaaaattt gaagtttgat ataattggga ttcacaatat
     2641 tgttgaccgt aatgaaataa agacagaatt agaaaaattc cttgcttcaa aagagggata
     2701 aatttaagat gataaggagg aatatttatg gagtttatta ttagtttgct gagtacacct
     2761 gctgttttgc ttggtgtagt tgcatgtata ggattgattc ttcaaaagaa atcagccatt
     2821 gaagtattta cagggtcttc aaaaacactt ataggatttt tgatatttgg tattggtgcc
     2881 tctgctatga caggagcact tcaaaatttc aataaattgt tccaacatgg ctttggaatt
     2941 actggtgtta tagcttcacc agaagcagct acagcacttg cacaaggtac atatggtttt
     3001 gcagtttcat gtacattaat tcttggattc atattaaatt tagtatttgc acgtattaca
     3061 aagtttaaga atattttctt tacgacaggt catagtttgt ttttttcttg tgtattagtt
     3121 ttaatcatga aagcacatgg attcgataat acaatcacta ttttaatagg tggaactatc
     3181 ttagggttta tgtctgctgc actacctcaa ttctgtcaac catttatgcg tgaattaaca
     3241 ggtggcgacg aacaagctat tggtcatttt aacatgattg gttatggtct atcaggttac
     3301 ataggacgtt tattttcaaa gcataaagat gatacaacag agactataga atttcctaag
     3361 tggttatcaa tatttagaga cttcttaatg ggtctatcca tagttatgtt aatcttgttt
     3421 tatattgcaa cattaaaagc tggtaaagta tttactcaag aaattgctgg ttcaacacac
     3481 tggttggtat ttccgcttat agaggcattt acatttgtgg caggtatgtc aatattgatg
     3541 tcaggagttc gtatgttctt agctgaaata actgcagcat ttgtaatcat ttcagaaaag
     3601 tatattccag gttcacgtcc tgcgctagat gttccaaccg tatttccata tgcaccaaat
     3661 gcagttatta ttgggtttat atcagcatat gctgctggtc tattagcaat tgctattatg
     3721 gcatcatttc ctagtatatt tcctgtagta attattccag ctgctcacat atgttttttc
     3781 tcaggaggta cagctgcaat atttggcaat acttcaggag gatggagagg ggctattgct
     3841 gggtcatttg ttgtggggtt attacttgca tttttaccag ttatactata tccagtttat
     3901 gggactcttg gtatagaagg agcaactttc cctaatattg attacaacgt agttggttcg
     3961 atattgcata atattttaca atttataaaa cctatttaaa ctaaattata taaaagtata
     4021 gtcatagtta ctatgatttc aaggaggata catcttatgg aaaatacatt atctgaattt
     4081 ttagataata tatctaatga attgtcagaa tttattgcct caatagatga gacatctata
     4141 aataaagcat gcgaattaat tcttgaagct gaaaaaaatc acggacgtgt acatgtgact
     4201 ggtattggca aaccaggaca tgtatcggga tatatttcat cattgctttc atctactgga
     4261 acgtcagcat atatattgca tggtacagaa gcagtacatg ggtcaagtgg tcaagtagtt
     4321 gaaggagatg ttgttattgc aatatcaaat agtggtgaaa ctcaagagtt aaaagcaact
     4381 cttaagactc taaaagtaaa tggagcaaag ataattggtg taagtggtaa tgagagttca
     4441 tttttaaaaa atatttctga tatattcctg tttgcaggtg ttaaacagga aggagattgt
     4501 cttaataaag ctcctcgtgc ttcaatttta gctgaaacaa taatattaca atcattaagt
     4561 gttgtattac aatatgctaa aggtcttaat acacagcagt atttgaagtg gcatccagca
     4621 ggaagtcttg gaaagtctat tagagaggga atataatttt atgaaatata aagggattat
     4681 atgtgcaatg ataacaccat ttgatgaaaa tcaaaatatt aattcacaag caacatgtca
     4741 attaattgat tatttgattg aaaagggaat ttatggatta tttattttgg gcactaatgg
     4801 tgaatgtcat gtattaacag atgatgaaaa agttgaattt gcaaagattg ttattaatca
     4861 tacaaataat cgtgtacctg tttttgttgg aacaggtggt aattcaactc gtgaagttgt
     4921 agatttatct aagagaatgg aagcgattgg agcaagtgca ttatctatta ttacaccatg
     4981 ctttgttaca ccaacacaac aggaattaat cttacattac aaaactattg ctaattcagt
     5041 taatttacct attataatgt ataatatgcc aggtaagact ggaattaata ttgaaccaga
     5101 gtcagtacgt gaattatcaa agataaagaa tattgttgga attaaggata gtagtggtaa
     5161 acttgacaac atgaaagctt atattgaagc aacgaagaat gagaatttta gtgtgttttc
     5221 aggctcagat tcacttattc tggatagtct aaaagctggt agtcaaggag ctgttgcagc
     5281 aacaacaaac ttcctcacag agattggtat ctctatctat gaaaacttta tgaaagggga
     5341 tttggaagaa gctcaaaaag ctcaaagtag tatagaagaa cttcgacgta tactgaaact
     5401 tggaacaatt ccatctgtta tgaagaaaac tattgtcttg aatggaatta atgttggaac
     5461 tgcacgactt cctgtaactg aaccaactgg ggaagtactt gaagaaatta aacgagtagt
     5521 tgaaaattat aggacaatac ttaataaaag tagtgagaat agataatata aatagggaaa
     5581 ttacactggt atataattaa tatatatatt ataaaacatg actaaaatta atgaataagt
     5641 aaagtggttt gatgagtata gaggtgataa tatgaaaaag atgaatataa ccaaaagaat
     5701 gtcagaatgt ggggctcttg caattgtaag agaagaaaat cttaaccgtg catgtgaaat
     5761 tgctgaagga tgtataaaag gtggtataac agttatagaa atgagttata ccataaataa
     5821 tgctggagaa ataattcaag ggcttaataa aaagtatggt gaaatacttt gtgtgggagc
     5881 aggaacagtt ttggatagtg aaacagctcg tcatgctgta ttacatggag ctcaatttat
     5941 tattgcacca aattacaatg aaggtgtagc gaagatttgc aatagatatc agattcctta
     6001 tgctccagga tgtacatcat tgactgaggc agtagatgca ctaagtttag gagcaacttt
     6061 tataaaagct ttcccaatat cagatttcta tggacctaaa ctagtaaagg tatttaagac
     6121 accaatacct gatatgccag ttttagcaag tggagggatt aatattgaaa acctacatat
     6181 atggttagaa aatggagttg atgtatgtgg atttggtggt ttattaacta aaggaactgt
     6241 tgaggatatt actgaaaatg ctaaaaagat tcgcgaaatc attaaaaata taagagaagt
     6301 ttaacatgga ggatgaatta gttttctatt gtcgagatat ccacacatta attcctaagc
     6361 tagaagaaat gttttctagt caaggtaaag tttatattga acttaaatta aattttgttt
     6421 taaataagat attattttag ttgattactt aaaatactag aatccatatt aatgaattac
     6481 actaattttg tactactatt aaaatattgt tttctaaaaa taaataataa ccttaaaaca
     6541 gttgattata aaaaattaac tgttttttgt tatttttatc tttgtgacga aatagacact
     6601 tgccatttag aatgtgacaa tatagtagtt gtttagaaaa gacacaaagc attatacttt
     6661 aactaaagta aaatgtattt tgcttttaga ataaaaataa ttagggagga gctataattg
     6721 aaaaagataa tatatgtact atttacaatt gaactattaa tagtatcttt attaggtcta
     6781 aatatagtta aagataatga aattaataat attttatata gtgatacaac aagtatatct
     6841 gtaatgttta aagattataa aaagttaaat aaaaattata gtaagtggat taaagatatt
     6901 gcagatgaaa ataatgtggc tatatcaaag tatgttttta ataataatga aaaattatct
     6961 atatatacga ctgatacaag tttgagtaac aatataaggt taaagtcagg aaaatttcct
     7021 aatattaatt caaatgaatt tattagtaat ataaacacta ataatagtaa tcaagtaggt
     7081 aaattctcaa ctatgagtaa agacatatca attttgatta gggattttgg taaaataagt
     7141 gaagttggag aatcaggaat cttatatatt tcaactcaaa atgaagatac aataaataaa
     7201 atattaaaag agttaaatgt ggacagaagt atttttgtag caagattaca tgatagatat
     7261 gttaactcaa atttatactt aaatccttca ataattagag atttaatact aattatcctt
     7321 tgtttcttgg ctacaataat acattattca attagtgtga gtagagaggc ttctatttta
     7381 agattaaatg gatactctaa gctagatata attgctagag ttataaaaaa tatacttaga
     7441 attatgattt tatcatctgt cacagcatta ttaatatata taatctatat tttaaaatta
     7501 aatttgggtg ttagaacatg cttatacttt gttatgtttt cgattatatg tattttgata
     7561 aatattttgg tatctgtatt aatagttggt tttaatagta gaagttctaa atatgtacta
     7621 tctctaaatg gaaaaaaatc ttatggtttt gtaaatataa tgcatttcac attgaaaatt
     7681 gtatttgtat catttcttat attatccata aataactgta ttttaaatta taatatgcta
     7741 caaactcagt tagggaatct atctgagtgg gataaagcaa aaaatgttta taatcttact
     7801 cttaaagata caggcgaaca atctttagga aaggaagaag taattaaaaa tgctaagatt
     7861 aagagtttct ataaaagttt agtagaagaa aaagatgctt tcttaattga tacaactaat
     7921 tttgaaaaac ttgaagatgg aagttacatg tatgaaataa attcaaaggg taaaaaccca
     7981 gaagtaagtc aatatggtaa aaatataaaa ataaataaaa actatcttaa agttaaccct
     8041 atttactcaa atggcaaaga aatatttaat ctaatcgatt ataacgataa gactataaat
     8101 ttattagttc ctaaaaaatt gcaagtacat gaaaatgata ttaaaaaaga atttagagaa
     8161 ttattttact ttgaaaaaat agaagtagaa aatatgtata atgagaagct aaatagaggt
     8221 ttaaataaaa caaaggaatc agatttaaat gttaacataa tatatgtaga taataatcaa
     8281 agttatttta cttataactc atttgttatg gaggataata gaaatttaat tgacgaccca
     8341 atagtaataa ttgacataga taatattgat gattcttttt atctttctta tatatctaga
     8401 tgtgtatatt ttaattctaa gaaattagat gcccttgcag atataagtaa cattattgaa
     8461 tctcaaggtg tagaagcgta tatccaaagt ttacatgcgg tttataatga gtatggttta
     8521 gaaataaata aactagaaaa atatttgaat agtgagattt ttactataat aataatagca
     8581 atatcaaact taatgattac ttacaatata gtagctagct actatgaaag aaataaatat
     8641 aagttatata ttaaaaaaat atttggttat tcaactacag ctagaagtat gttgttaata
     8701 gtctcattaa tattaacaaa tattattcca ataagcatta tatcaacaag agtagattta
     8761 agtaacaata ttatcttatt tggatttctg atattagttg tagaagtaat tatatctatt
     8821 atattagaca agattatctc aaatagttca tttaataaaa ttataaaagg agaacattaa
     8881 aaagatgatt tcattaataa atatatcgaa aaaatttaaa gataaatcta taatagacaa
     8941 atttaaccta gaaatttcta agggtgagtt tgtagcgata ataggaaata gtggttcagg
     9001 taagacaact ttacttaata taatgggatt attagaaaaa cctaattctg gtgatattat
     9061 aataaataat ataaaaaatc caaatagtaa acagataaga attttacaaa gggattttta
     9121 tggttatcta tttcaaaatt atgctttaat tgagaatgaa aatgttgaaa ataacttgaa
     9181 aattgcttta aagtatcaaa aaaatatcaa ccataatgaa aaaataaatt acgcacttga
     9241 gtctgtaagt cttagaaaat acaataaaaa aaagatatac gaattgagtg gaggagaaca
     9301 acaaagggta gcattagcaa gagtaatatt aaaagaatgt gaagtaatat ttgcagatga
     9361 acctacaggc aatttagata aaaaaaatag agacatagta tttgatatac ttaaaagtct
     9421 gaataagaat gggaaaacta tcgtatttgt aactcatgat ttagagttat cggagcaggc
     9481 agatagagtt ataaaattgt aataatatca actataataa atataagaaa gagattacag
     9541 ctaaatcaag tttaataata ctacgaatag aatttaaaca ttaagtaaat tagtagtttg
     9601 taaatataag tataatatga tatcataaag gctcaaaaga aattttatat aataagaaat
     9661 ctaaaaataa caaatggagg acatataaag atggaaaatt taccaaattg cccgaaatgt
     9721 aattcagaat atacttatga agatggaact cttttagttt gtccagaatg ctctcatgaa
     9781 tgggcattag gattgggagc tgaagaagat tcaaatatta tcaaagatgc aaatggaaat
     9841 gtattaaatg atggagattc tgttacagta attagagacc ttaaagtaaa aggaagttca
     9901 tcatctataa agattggaac aaaagtgaaa aatatccgtt taatccatga ctcatcagat
     9961 ggacatgata tagaatgtaa gatagatggt tttggagcta tgaagctaaa gtcttctgtt
    10021 gttaaaaaag cgtaatatta taaataagct gtagtgttta ttgtttttca gtacagtata
    10081 aaaccataag aacctattaa gtaataaaag tttgtttatt tatctttata ttttcttatg
    10141 gttttattaa atatagattt tattgaacac caaaatattc tctcctttac aaataagcca
    10201 atcttattta aactaatata ctttatacta tttatactga aattgtaaat ttaaatgcta
    10261 tgttatttag gtttatatag ttaatttata tatttctgat tttataaatt gtatttatta
    10321 ttatatctta attctaaaat cattaaaaat ttcaatctct ataaagattt acaatttctc
    10381 aaataaatta ttagtatata aacaatttat tattacagtg ctgcatttaa aataagtctt
    10441 gatataaagg tattgttaca agtataaaga aacttatgta tgctattgga ataaataata
    10501 ccttaaatta taattaagaa taagaactgt taaataaggg acttgattat catttagtta
    10561 tagataatat aatattaaaa ataaagctta agaggagaaa aaatatgata gatatacata
    10621 aaaatatata tgataataag ttatttgagg agctaaagat tgactgtaaa aagtgttttg
    10681 gactttgctg tgtagctctt tatttttctg cttcagatgg atttcctatt gataaggaat
    10741 caggaaaacc ctgtagtaat ttacaaccag attttaaatg ttctgtacat aatagtctta
    10801 tgaagcgagg ttttaaaggg tgtactgcat atgattgttt tggttcaggt caaaaagtag
    10861 cacaagtaac atataagggg atagactgga tgcaatcacc agaattaaca aatcaaatgt
    10921 ctgaagtgtt tttaattatg agacaattac atgaaatgtt atggtatctt aaagaagcat
    10981 ctgtattaaa tatatcagat acaatcaaat caaaaattga tttaataatt gaagaaactg
    11041 aaaagataac taatatgagt ccagaacaga taattgattt ggatatcata tcacatagaa
    11101 caaaggttaa tttattatta tcacaagcga gtgagtctgt gatgggaaag gttaaatcat
    11161 ttatcaagac ttctactctt aaaaacatga aaaaattaag taaaaatata gatttaatag
    11221 gagcagacct tagaaaaaca aatctaatag gagcagattt aagaggtaga tttctaatag
    11281 cagcaaattt aagaaataca gatttgagtg gggcaaattt aataggagca gacttaagag
    11341 attgtgatat tagaggtgct aatcttgaaa atagtatatt tttaacacaa ttacaagtaa
    11401 atactgcaaa aggagattct agtacgaagt tgccagcttc actaatacgc cctaaatact
    11461 gggagaaata attaaattaa tatataattt tacaagatat gaactttttt gactttaatt
    11521 catattatac attagagatt ctttacaaga atttcttaat ataatatagg gagagatata
    11581 aaaatgagtt atcaaaatta taaatgtgaa agagattatg tgtatgatga ttataaatac
    11641 aataagtgca acaagtgcta taatgattat gaggaaatga cacatgttca tgaatattca
    11701 gaaagtgtta agttagcaga agaatgtgag gatagacata accaccgtgc agcaggagtt
    11761 actggtgaag ttataccaat aaatggaggt acaaatcatg ttcataaaat aaatgataat
    11821 gtagatttcc ttgaccattt ccataagata tgtgttacaa cagggccagc aattagaata
    11881 cctggaactg ataaacacat tcacttaata tgtggagaaa ctactgttaa tgatggtcat
    11941 tgtcataaat tcctttttac tactcaaata gaagctcctt tagtataagc tgatgtgaat
    12001 tattgtagta aatttgctag attatagtat atagatgtta aaaatatata atagataata
    12061 tttaaactta atgaggacta tgatatttaa ccttcgtagt cctttctaag ttttgtatga
    12121 agaagtgtta tacaattgaa agtgatatag ggagaaaatt tttatgaaat ctgaaataat
    12181 tcttgaaatg aaacaaataa attcaacaga atatgacgaa atgttgcaag tctgggaatc
    12241 gtcagtaaga gcaactcatg attttttaac aaaaaaagat attgaatctt tgataccatt
    12301 agttgaaatt ggacttaaag aagtagaaaa tatagtttgt ataaaagata atgatataat
    12361 aaagggcttt ataggaatag ataaagataa aattgagatg ctctttatag aagataaata
    12421 tagaggaaat ggtattggta aaaaattaat aaaatatgct atagataaat acaatgtaaa
    12481 atatgttgat gtaaatgagc agaataaaaa agctgtagga ttttatattc acttagggtt
    12541 taaagcgttc gataggtctg aaatagacgg acaaggtaac ccatttccaa tactacatat
    12601 gaaattaatt taaagttata cttctcactt attcgcaaaa atatttactt aaatttttta
    12661 tacaaataaa agtcgtgaca gtcttaggac acgactttct tactacacta ttaaattgaa
    12721 atttatctgt tctatattat gattattaga tgtagttttt ttattttaac tatgtactta
    12781 aataccaagt atacccatac ctatcaagta cttcagcagc gccatcattt tgatgtggtg
    12841 gtgggtgtgg taaaaattct tgtacaagcg taccaccctc ggcaagtttt acagatattt
    12901 cttctattgc ttctcgagat ggtagaacaa cacaaatcgc catgccagaa attacaacag
    12961 gcttgtctgt atccgcaccc attatacaac cttcatattt tgtgaaagta acacttgcgt
    13021 gcataacagg attgtttgga ttgctgtcta atcgcgcaaa tatagttttt cctcctccga
    13081 aagcctcagc ataaaaatta aatgcttttt cacaagtacc attaaatatt aaatatggag
    13141 ttagcatgac aaatatatcc tccttgatat agaataattt atagttgcac ctgatagtct
    13201 aaattctgat ttagtaaatt gtatcatagg taaaccttga tgtatataat tttttattat
    13261 tagattatac aaatttatta attaaaaaaa atgttatatg tatggaagat ttacaggata
    13321 aaatataata aaaagattaa gatgacatta taaaaaatgt atattgacat tgacgctacg
    13381 taattgtata tactttgtct taaaggagat ggtaaatatg aatattatag aaattcgctc
    13441 agataaaata tacaagaaga taatggattc accaataaac aaaaaagaag atatatacag
    13501 atatgaattg atgaagcctt ttgaatttaa gtggaagtgt atgaatgttc caatagttgc
    13561 taaacagaaa ggcggatatg atgtaattat agcaagtgaa atgttagggg ttttatcgcc
    13621 taaggatatt gatgaaaagc aaaaaaagaa tataaatgtg ttatctgctg ataaaatttg
    13681 gaacacttgc aaagaaacta tagaaaactc tataaatgct tttataaaag aagggtatga
    13741 tttaaacatt aaggattata aatattcaat attattggcg aatccaaata gttcttacac
    13801 aatattaagt gatggatact ggggtgatgg tgggattcct ggatatatat ttctatcatt
    13861 ggttcctaat gaatatacta tcaatagatt accagtatta atagcacatg aatgtaatca
    13921 caatattaga ttccagttta tagagtggaa taataatata acattagaag aaatgatgat
    13981 aaatgaaggt cttgcagaaa attttgcaac atggatgttt ggagatgaaa tggtaggacc
    14041 ttgggtcagt agaatagata tcgaaacact aaatacgtat ataaagccaa taataaaaag
    14101 tgctttaaaa gaaactggat ttcaaaatat aacatcttat ctttatggtg atgatatagc
    14161 taaaatgcaa ggatattttc cagtagggtt gccttattgt gcaggatatg cttgtggata
    14221 ttatatgatt aagtattatt tagaaaagac aaataaatca ataatcgaag ccactttatt
    14281 gccttatagt gagataatag aagcagtaaa agagttttgg gaataaattt tataaattgc
    14341 ataatttgaa ataaattatg caattatttt atttttaaga aaaaataaat tgacaataat
    14401 tt
//
LOCUS       DAEPXF010000093        14348 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain HB27
            SAMN13376389-rid12479423.denovo.098, whole genome shotgun sequence.
ACCESSION   DAEPXF010000093 DAEPXF010000000
VERSION     DAEPXF010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13376389
            Sequence Read Archive: SRR10518432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 14348)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14348)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001069624.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001069624.1
            Genome Coverage       :: 315x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/15/2021 01:54:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,165
            CDSs (total)                      :: 4,076
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 89
            rRNAs                             :: 10, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 10 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 19 of 69
            Pseudo Genes (incomplete)         :: 44 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 10 of 69
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14348
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13376389-rid12479423.denovo.098"
                     /strain="HB27"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="China: Hebei"
                     /collection_date="2017"
     gene            complement(227..1420)
                     /locus_tag="KRL67_002405"
     CDS             complement(227..1420)
                     /locus_tag="KRL67_002405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005336078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HBH1523279.1"
                     /translation="MKEKRRDSKGRILHTGESQRTDGKYLYKYVDAFGNTKYVYAWRL
                     TPTDPTPKGKREKPSLRELEQQIRRDIEDGIDSTGKKMTLCQLYAKQNTQRANVKKST
                     QKQREQLMRLLKEDKLGARSIDTIKPSDAKEWALRMKDKGFSYNTINNHKRSLKASFY
                     IAIQDDCVRKNPFDFKLSEVIENDTKEKVALTEEQEQALLSFIKADNVYHKYYDDVLI
                     LLKTGLRISELCGLTVADIDFKNEVVIIDHQLLKSKEQGYYIETPKTKSGARQVPLSR
                     ETIQAFQRVMKKRPKAEPFVIDGRGNFLFVNHKGKPKVAIDYNMLFVRMVEKYNKHHK
                     DNPLPHITPHTLRHTFCTRLASKNMNPKDLQYIMGHSNISITMNWYAHASIDTAKSEV
                     QRLIA"
     gene            complement(1499..1702)
                     /locus_tag="KRL67_002406"
     CDS             complement(1499..1702)
                     /locus_tag="KRL67_002406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002262306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HBH1523280.1"
                     /translation="MNNNDIPVWEKYTLTIEEASKYFRIGENKLRRLAEENKDAGWLI
                     MNGNRIQIKRRQFEKVIDKLDAI"
     gene            complement(1702..2793)
                     /locus_tag="KRL67_002407"
     CDS             complement(1702..2793)
                     /locus_tag="KRL67_002407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018594174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HBH1523281.1"
                     /translation="MTTHKNLSVKIDYISIVFETATAEDVIMHILDLPTDIFNVYPAT
                     IKFKTYQARWQIGDIYVSGDARKTEDNPQGLGCYLVMTGRGCDDIFRILDSRNYTFGD
                     MFRRCERRYGLDNFHFTRLDIAIDDKNEKPFFTIEQIKKKCEKEEFISNSEGYHFDES
                     KFDDFDTAKTVYIGAGKSGLSYRFYDKDKEVCSKHNKTLDEVGSWKRTEMQLRDDKAH
                     AFAMTFKDRPLELGELAFGLLANNLRFVVPNRNESNKSRWKTCRFWERFLGAMEVLKL
                     QVPKLHNSLEETQQWLTEGGVISAVKSFYFLEEHDALGGLEKVGTMLDKARYSNSLSS
                     KLTAHLQRIDRTDLIPYIQYNTKHGKGGI"
     gene            3265..3900
                     /locus_tag="KRL67_002408"
     CDS             3265..3900
                     /locus_tag="KRL67_002408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBH1523282.1"
                     /translation="MEHYEKKISFLGENIQTIRKHRGMKQQELADKIGINMQSLSKIE
                     RGVNFPTFDTLEKIMDVLGVMPNELLSGEWKYVNQSEKEVCQFLRIEERLNAELKHGH
                     YDNFFDSEEEWLEYELEKLREYITDYINGKSIVASDLYPIKEFIQHLKFQKLLDRYDD
                     LYSMDMFGESIEGHKYRTPYQVVKMINPNSKEDMELLREVLKNNHFDDEDE"
     gene            4251..4862
                     /locus_tag="KRL67_002409"
     CDS             4251..4862
                     /locus_tag="KRL67_002409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008728833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HBH1523283.1"
                     /translation="MNITKQKSQKTFDKQAADYDTNIQGEHARKLYKPIIENLKNKNI
                     HSILDLGCGTGALLKEIKELNIAEQLFGIDISPNMLEIAKNKLGNDATLILGDSERLP
                     FEDSSFDAIVCNDSFHHYPQPDIVEKEVSRCLKQNGVFIIGDCWQPIGARQIMNYYMK
                     HSNSGDVKIYSKKEMLLLLSKDFHEIEWKSFGTRSCLIIAYNN"
     gene            4886..5446
                     /locus_tag="KRL67_002410"
     CDS             4886..5446
                     /locus_tag="KRL67_002410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008728836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBH1523284.1"
                     /translation="MRISKEPEERKQEILETAIKLFSVNGFEKTSISDIAKEIGIAQG
                     LCYRYFPSKDVLFQSAINEYSNILVANMTKNINIKKDSLKDILRKMILFAEQEDDTYY
                     PVFHNNQNKNFHNLLTLNVCEKLFPIVSEIIERANYTNEIQVNDVEMYASFCIYGQLG
                     IILNTDISIKEKSSRIKAFFRDLFRL"
     gene            6074..7123
                     /gene="cfr(B)"
                     /locus_tag="KRL67_002411"
     CDS             6074..7123
                     /gene="cfr(B)"
                     /locus_tag="KRL67_002411"
                     /EC_number="2.1.1.224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(8))-methyltransferase
                     Cfr(B)"
                     /protein_id="HBH1523285.1"
                     /translation="MQQKNKYIRIQEFLKQNKFPNYRMKQITNAIFPGRINNFNEITV
                     LPKSLRDMLIEEFGESILNIVPLKAQQSTQVSKVLFGISGDEKIETVNMKYKAGWESF
                     CISSQCGCNFGCKFCATGDIGLKRNLTSDEITDQILYFHLQGHSIDSISFMGMGEALA
                     NVQVFDALNVLTDPALFALSPRRLSISTIGIIPNIKKLTQNYPQVNLTFSLHSPFNEQ
                     RSELMPINERYPLSDVMDTLDEHIRVTSKKVYIAYIMLHGVNDSIEHAKEVVNLLRGR
                     YRSGNLYHVNIIRYNPTISSRMRFEEANEKCLVNFYKELKSAGIKVTIRSQFGIDIDA
                     ACGQLYGNYQKTNSQ"
     gene            7824..8765
                     /locus_tag="KRL67_002412"
                     /pseudo
     CDS             7824..8765
                     /locus_tag="KRL67_002412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008728838.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
     gene            8957..9376
                     /locus_tag="KRL67_002413"
     CDS             8957..9376
                     /locus_tag="KRL67_002413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008728841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HBH1523286.1"
                     /translation="MNPSSFEHIVRIQFNALMMTVIKCTVKNRNRQFARRSKREVLFC
                     ELSDTKNVECVTKDNYSCDYISFEVLNFTIQISNEKLAVALYKLSNKERDVILLRYFQ
                     SMSDQEIAELYHVSRSAIYRRRSNGLKKLKTVLKERN"
     gene            9381..9620
                     /locus_tag="KRL67_002414"
     CDS             9381..9620
                     /locus_tag="KRL67_002414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004609551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBH1523287.1"
                     /translation="MKKEYGYPTLKVICQASAGNETAIREILKFYDAYICKLCLRPFY
                     HSESGKIIMQVDEELKGQIHTEMMKAILKFEIRVK"
     gene            9753..10152
                     /locus_tag="KRL67_002415"
                     /pseudo
     CDS             9753..10152
                     /locus_tag="KRL67_002415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434143.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
     gene            10140..11078
                     /locus_tag="KRL67_002416"
     CDS             10140..11078
                     /locus_tag="KRL67_002416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HBH1523288.1"
                     /translation="MENLILSINVVLPLFLTMSLGYVLKKLGLFDTHTLNKMNNVCFK
                     SFLPLLLFFNIYNTDLDGSLNFKLMAVAVIIIFSIFLILVFIIPKIEKDNKTRGALIQ
                     GVFRSNFVIFGIPVTISLFGDKSIGVTSLLIAVIVPMFNVLSVVILEIFRGGKVSIKH
                     MIKSVLTNPLIIASFIGLTMLILNINIPTFLQKTIGDISKIATPLSLIILGGSFSFSA
                     IKGHIKPIILGVCGKLILVPAIFLPISIFLGFRNVELASLMIMLSAPTAVSSFTMAQQ
                     MDSDSELAGQIVVFTSAFSIITVFLIIFILKQNNLI"
     gene            complement(11444..11668)
                     /gene="secG"
                     /locus_tag="KRL67_002417"
     CDS             complement(11444..11668)
                     /gene="secG"
                     /locus_tag="KRL67_002417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HBH1523289.1"
                     /translation="MSNILMGVQVVLGILLVISIMPQDSKNALPSEFGGEGSQAYFKP
                     KGKQAFLARVTKITAVLFFINALALLIVKK"
     gene            complement(11782..12291)
                     /locus_tag="KRL67_002418"
     CDS             complement(11782..12291)
                     /locus_tag="KRL67_002418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBH1523290.1"
                     /translation="MNLEIIRATGENATEMGFIHSYSWKKAYKGIIPDNIIDEFTSEK
                     RAEIFHNVIPKAEEEYYLFKVDGIPAGFASLNKSHEENAPKYIGEIYSIYFHPDFWGT
                     PVTKKGLQFCIDRLRNLGYSYITIWVLKDNTRAIKFYKKNGFTCDDFEKEIYIGKKLL
                     EIRYSKKIQ"
     gene            12371..12802
                     /locus_tag="KRL67_002419"
     CDS             12371..12802
                     /locus_tag="KRL67_002419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HBH1523291.1"
                     /translation="MSMNAIKELSNLWHIVGMQMKTEMCSGKYPTIYGISLVELSILE
                     IVEQHPNSMMKEISSTLELPKSTLTSAVKRLELKKLIKRNPCIKDKRAYNLDLTELGL
                     KAQQEHREIEATVFKNILHQLDKDEIETFIHIFSKAVNKIR"
     gene            complement(12902..14194)
                     /gene="eno"
                     /locus_tag="KRL67_002420"
     CDS             complement(12902..14194)
                     /gene="eno"
                     /locus_tag="KRL67_002420"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HBH1523292.1"
                     /translation="MSVIELVYAREVLDSRGNPTVEVEVVLEDGAMGRAIVPSGASTG
                     AFEAVELRDGDKGRYLGKGVETAVANVNEIIAPEIEGMDAFDQPAIDAIMIELDGTPN
                     KGKLGANAILGVSMAVARAAADEIGLPLFQYLGGVNAKQLPVPMMNILNGGEHADNNV
                     DVQEFMILPVGACCFKEGLRMGAEVFHSLKKVLGEKGLACGVGDEGGFAPNLGSNREA
                     LELIVEAITKAGYKPGEDVMLGLDVAATEMYNKETKKYVLAGEGKELTAAEMVALYED
                     WSNNFPIITIEDGLDEEDWDGWKLLTEKLGNKLQLVGDDLFVTNTERLEKGIENGVAN
                     SILVKVNQIGTITETLDAIEMAKRAGYTAVISHRSGETEDSTIADLAVAVNAGQIKTG
                     APSRTDRVAKYNQLLRIEEMVGEQARYCGLKSFYNLKK"
BASE COUNT         4713 a         2337 c         2378 g         4920 t
ORIGIN      
        1 tcatgataaa aaaaatagag catgtggtca ttactaataa taatatagaa aattaatata
       61 aattatatat gtctttttga ctatcttttt atatcttctt aaatgtccgt aaagccttat
      121 tttatcggct ctacgggcat tttgcttttg ttgtaaacct cacatatcta ggtctatctt
      181 cttatatttt cgctatcaag cgtggttaaa atcgtggtaa atacttctat gcgattagac
      241 gctgaacctc tgattttgca gtatctatgg acgcatgagc ataccagttc attgtgatac
      301 tgatgtttga atgtcccatg atatactgta aatcttttgg gttcatgttc ttacttgcca
      361 gccttgtgca gaacgtatgg cgtagtgtat gcggtgtgat atgtggcaag ggattgtcct
      421 tatggtgctt gttgtatttc tctaccatac ggacaaaaag catattgtaa tcaatcgcaa
      481 ctttgggctt gcctttatga tttacaaata gaaagttgcc ccgtccgtct atcacaaatg
      541 gttctgcctt tgggcgtttc ttcataaccc gttgaaatgc ttgtatcgtt tctctgctta
      601 atggcacttg ccttgctccg ctttttgtct taggcgtttc aatatagtag ccctgttcct
      661 tgctctttaa taactggtgg tcgataatca caacttcatt cttgaaatca atatcggcta
      721 ctgtcagtcc gcacagttcc gagatacgaa gtcctgtctt taacagtatc agcacatcat
      781 cataatactt gtgatacaca ttgtccgcct tgatgaatga gagtagggct tgttcctgtt
      841 cctctgtcaa tgcgactttc tctttggtat cattttctat aacttcactt aacttgaaat
      901 caaaagggtt tttccttaca caatcgtctt gtatggcgat atagaatgac gcttttaacg
      961 agcgtttatg gttgttaatg gtgttatagg aaaagccttt gtctttcatg cgtaacgccc
     1021 attccttagc gtcagagggt tttatcgtat caatgctcct agcacctaac ttgtcctctt
     1081 tcaataaccg catgagctgt tcccgttgtt tctgtgtgct cttcttcacg tttgccctct
     1141 gtgtgttctg tttggcgtag agttggcaaa gcgtcatttt cttgcctgtg ctgtcgatac
     1201 cgtcctcaat atcccgtctt atctgctgtt ccagttcacg aagtgagggt ttttcccgtt
     1261 ttccctttgg tgtcgggtct gtgggtgtca atctccaagc atacacatat tttgtgtttc
     1321 caaatgcgtc cacatattta tataagtatt tcccgtctgt tcgttggctc tctccagtat
     1381 gcaggatacg tcctttgcta tcccgtcttt tttctttcat ggtgtctgct cctttccttg
     1441 ttggaaagag ccttgatatg acttgtattc atcataacac atacaaggct catttgcatt
     1501 agattgcgtc caatttgtca atgacctttt caaactgtcg gcgtttaatc tgtatgcggt
     1561 tgccattcat aatgagccag ccagcgtcct tgttttcctc tgccaatcgt cttaacttgt
     1621 tttctccgat acggaaatat tttgacgctt cttcaatggt aagggtgtat ttttcccata
     1681 cgggaatatc gttgttattc atcagatacc ccctttccca tgtttcgtgt tatattggat
     1741 ataggggata agatcggtgc ggtctatcct ctgtaagtgg gcggttagtt tactggaaag
     1801 ggaattgctg tatcttgcct tatcaagcat agttcccact ttttccagtc cacctaatgc
     1861 gtcatgttct tccaagaagt aaaaactttt cacagcggaa atcacgccac cctctgtgag
     1921 ccattgctgt gtttcctcaa gagaattatg tagtttcggt acttgcagtt ttaagacttc
     1981 catagcccct aaaaagcgtt cccaaaaccg acacgttttc catctgctct tattactttc
     2041 atttctgttt ggcacgacaa agcgtaggtt gtttgccaat agcccgaaag ccagttcccc
     2101 aagttccagc ggtctgtctt tgaatgtcat ggcaaaagca tgagccttat catctcgcag
     2161 ttgcatttct gtccgtttcc aactgccgac ttcatcaagt gtcttattat gttttgaaca
     2221 gacttcctta tctttgtcat aaaagcggta ggacaatccc gatttaccag caccaatata
     2281 aacagtcttt gcagtgtcga aatcatcaaa cttgctttcg tcaaagtggt agccctcact
     2341 attagaaata aattcctctt tttcgcattt cttctttatc tgctctatgg taaagaatgg
     2401 cttttcgttc ttatcatcaa tggcaatatc aagtcttgtg aaatggaaat tatccagtcc
     2461 gtatcttcgc tcacagcgtc ggaacatatc cccaaaggta taattcctac tgtcgagaat
     2521 acggaaaata tcatcacaac ctctgccagt cataacaaga taacagccta gcccctgtgg
     2581 gttgtcctct gtctttcttg cgtcccccga tacataaata tctccaatct gccagcgtgc
     2641 ctgataagtc ttgaatttta tcgttgctgg ataaacattg aaaatgtcag tcggtaaatc
     2701 taaaatgtgc atgattacat cttcagcggt tgccgtttca aatacaatgc tgatgtaatc
     2761 aatcttaacg gataaatttt tgtgtgtagt tatagtgcat ttctcctttc gtatgccccg
     2821 tttttttgcg gggtaaaaat tgtgtttttt cctttattta tcacagtttc taggtactat
     2881 gacatgtata cgcagggcgg tgttacgcag ggcggtgtta catatacgcc ctctgcgaac
     2941 gcctttaggc gttccttttc caaaaggaac tgtctgtccg aacacgtctg ggggacgtgc
     3001 cccgtccagc cagccccttt tggaaaccac tattttcggt ctgatagtcc atgcttattc
     3061 atgctatcaa agtgaaaatg tatcttctct atgcttactc aagcgtgtga ttgaccttat
     3121 cgtgcttaat cactctatct gatacgataa taacgccatt agaattactt gtcaagaagt
     3181 ttttgttgac ttttagatgt agattaagta tgatatgctt ataaatacca agaaaagcaa
     3241 cgaaaggagc ggttatacta agttatggag cattacgaaa agaaaatcag tttcttagga
     3301 gagaacatac agaccataag aaaacacaga ggaatgaaac aacaggaact tgcggataaa
     3361 atcggtatca atatgcagag cctttccaag attgaacgag gtgtgaattt tcctaccttc
     3421 gatacattag aaaagataat ggacgtgctg ggtgtaatgc ccaatgaatt attatcggga
     3481 gaatggaagt atgttaatca atccgaaaag gaagtctgcc agtttttaag aattgaagaa
     3541 cgattaaatg cagaactaaa acatggacat tatgacaact tctttgacag cgaggaagaa
     3601 tggctggaat atgagttgga aaagttacgg gaatatatta ccgactacat caacgggaaa
     3661 agtattgtag cgtccgacct ttatccaatc aaagaattta tccagcattt gaagtttcaa
     3721 aaactgttag accgctatga tgatttatac agcatggata tgttcgggga aagcatagag
     3781 ggacataaat ataggacacc ttatcaagtc gtaaaaatga taaaccccaa ttcaaaagaa
     3841 gatatggaat tattacggga agtattgaaa aataaccatt ttgatgatga ggacgaataa
     3901 ctttttccct ctttgtagaa gatacagcaa acaaaaagcc cagtaaagag tgcaaagcac
     3961 caccaatggt ggcaaggctc ttgacagggc tttttctttt ctgtggaggg taatcaagag
     4021 gaagaaaaga taaaatttga tttgtggtaa atcggataat atcaatgaaa ttgtcacggt
     4081 acaaatttgg gctatataag gaatagtaac tggaagattg tagggtaatc aaaaaggcgt
     4141 gtattttaaa tcagagatac acgtctttga aatgaatgaa attcaatgaa ttttattcat
     4201 tgacaatcgt tcattcacga gatataattt tagtgaaagg agttgattac atgaatatta
     4261 cgaaacaaaa atcacaaaag acttttgata agcaagctgc cgattatgat acaaatattc
     4321 agggggaaca tgctcgaaaa ctctataagc cgattataga aaatttaaaa aataagaata
     4381 tacattctat attagattta ggttgtggaa cgggtgcgtt acttaaagaa ataaaagaat
     4441 taaatatagc agaacaacta tttggaatag atatatctcc caatatgctt gaaattgcaa
     4501 aaaataaact aggaaatgat gcaacactta tcttaggcga ctctgagaga ttaccttttg
     4561 aagatagttc ttttgacgcc attgtatgta atgactcatt tcatcattat ccacaaccag
     4621 atattgtaga gaaagaagtg tctaggtgtt taaagcaaaa tggagttttc attatcggtg
     4681 attgttggca accaatcgga gcaagacaga taatgaatta ctatatgaaa cacagcaata
     4741 gtggagatgt aaagatttat tcaaaaaagg aaatgttgtt attattatca aaagacttcc
     4801 acgaaattga atggaaatct tttggcacaa gatcttgttt aattatagca tacaataatt
     4861 aaaccgcaag aaaggaaaga aaacaatgcg tatttcaaaa gaaccagagg aacgtaaaca
     4921 agaaatttta gaaacagcca ttaaattatt tagtgtaaat gggtttgaaa aaacttcaat
     4981 atcagacata gcaaaagaaa ttggaatagc acaaggatta tgttatagat atttcccatc
     5041 aaaagatgta ctttttcagt ctgctatcaa tgaatattca aatattttag tcgccaatat
     5101 gacaaaaaat ataaatataa aaaaggatag tttaaaagat attttgcgga agatgatact
     5161 ttttgcagaa caagaagatg atacatatta tccagttttt cataataacc aaaataaaaa
     5221 tttccataat ttattaacat tgaatgtatg tgaaaaactt tttcctattg tatctgaaat
     5281 tattgaaaga gcaaattaca caaatgaaat acaagtcaat gatgttgaaa tgtatgccag
     5341 tttttgcata tatggacaac taggaattat tttaaatacc gatatatcaa ttaaagaaaa
     5401 gagttcacgg ataaaggcat tttttaggga cttgtttagg ctttaaatct tcccctgcca
     5461 aaaggcaggg aatatttatt ataattcgtc catacgaatt acctcctgca atatagtcag
     5521 agaggaaacc cctctaccta atagccacag gagataatga ttgttgagga ttttgaaaaa
     5581 attaatgggt atttggatta ttgtgcggaa tttgtgtatg ttgaaatgga tttaaaattg
     5641 attcagcaat ttttctactt taacaaacgc cctttaattg aataacttac tttaaagaaa
     5701 actggatatt tatgttgaaa taagaacaaa attgtgaatg gtttaactta aatgttaacg
     5761 gtaaacggtg caggttagtt taactacatt tatatccaaa attgtttgtt tttcaaatta
     5821 catgtatttt tttgagttta catagggaaa gatttatggt atactattta acacttatat
     5881 tgacaagaaa gaggtgaaag caatgttaac tgtttttaga attttgtttg gtaaaaatgc
     5941 aaaagttggt aatcgttcgg catacaaagg gagtagtctg tcctttttta agccatataa
     6001 ccatacaaaa acagttatcg gagcttttcc tctacggcaa acaaaccaga gttatatgga
     6061 aagatgagaa aacatgcaac aaaaaaataa gtatataaga attcaagagt tcttgaagca
     6121 gaataaattt cctaattata gaatgaaaca aattacaaat gctatattcc cagggagaat
     6181 aaataatttc aacgaaataa cggttcttcc taaatcacta agagatatgt taattgagga
     6241 gtttggagaa tcgattttaa atattgttcc tttaaaagca caacaatcta cacaagtttc
     6301 aaaagtctta tttggaattt caggagacga aaaaatagaa acggtaaata tgaaatataa
     6361 agctggttgg gagtcatttt gtatatcatc gcagtgcggt tgtaattttg gttgtaaatt
     6421 ttgtgcaact ggagatatag gtttaaaacg taacttaact tcagatgaaa ttactgacca
     6481 gattttgtac tttcacttac aagggcattc aattgacagt atttctttta tgggaatggg
     6541 agaagcatta gcgaatgtac aagtttttga tgctttaaat gtacttacag atcctgcgtt
     6601 gtttgcttta agtccgcgta ggttatctat atccactata ggaattattc caaacattaa
     6661 aaaattgact caaaactatc cgcaggtcaa cctgacattt tcattacatt ctccttttaa
     6721 tgaacagcga agtgagttaa tgccaattaa tgaacgctac ccattatcag atgtgatgga
     6781 tacattagat gagcatatac gagtaacctc aaaaaaagtt tatattgctt atattatgtt
     6841 gcacggagtt aatgattcta ttgaacatgc gaaagaagtc gtaaaccttt taagaggtag
     6901 atataggagt gggaacttgt atcatgtgaa catcattaga tataacccga ctattagttc
     6961 acggatgcgg tttgaagaag caaatgagaa atgtcttgtc aacttttata aagaattaaa
     7021 gtcagcagga attaaagtta ccattagaag tcaatttggc attgatatag acgctgcttg
     7081 cggtcaattg tatggaaact atcaaaaaac caatagccag taacttgagg tgagagtgat
     7141 gtttagaacg tgggaagtac cctaataatt tatttagtaa cgataaataa acttattagg
     7201 atatttatga taaaaggaat cttagaaaga aaaataggca tcactcaagt ggagctaaaa
     7261 agtcgtttgt aaaaattgaa actgcaatta aattattgaa tgtgaaaaat ggtgatgatt
     7321 agtgttaaac aaaaaaataa tttcagtcaa agtatcagta gagatcatgt agtcattacg
     7381 gatttttgga cgttgactga caataatgaa attcaactac cgtccaacat ggaaattata
     7441 tgcaataatt atttgaagag tttgaaattg atgtatcaga agattactct aaacagtagt
     7501 tggaaaaatt tgtcatgcga gaattgcgga tgcagtgaat tattgataat caggtggaag
     7561 acagtagtgt atccaatgaa gagatgaaca cgaataccaa aaatcactta aaataaggtg
     7621 gatatgcaaa aggaatacaa tcgtgttatc cttgatatga aaagcaagaa aagaaatggg
     7681 aaacatttgt tctcaattta aaaaggcaac gtcaattgaa atatttataa tttcgcttat
     7741 ctaaaaggcc ttagagcata ttttaatgaa caaaattgaa aggactgaac ctataataat
     7801 gattcactaa tccataaact attttgtatt tatactttac agccatttta agaagttttg
     7861 gcaatactat gtttcaagca gttgcaatgc ttgtgtctac tttattgaat gctattttag
     7921 atcctatttt cattaacata attggatttc acggagcggc aatcgcaacc ttaatatcac
     7981 agattatttg tttggcgttt atgtgcattt atatttatcg taaaaaatta tttaaccttt
     8041 cactatctga ttttgataca aaagagatta tgccgtttgt aaaaaatgct gtgccgtctg
     8101 tcattcaaca aagtattcct gctattagta caacattttt aactgctatt gttagtactt
     8161 atagtatttc tgctattgct ggctacggag ttgctggaaa attagaaatg atattatttt
     8221 atcctgctat ggctcttaat atggtgttga cttcaatcgt agggcaatgt attggagcac
     8281 aacgtatcga cagagcaaag aattatctga aattggcgtt aaaatatggt attgttgttt
     8341 caagtgtatt atcagcagtt gttattttct tctctaaaca gttggcaaaa ttgtttgttt
     8401 ccaacgtagc tgtaacaaat gttgtcagcc attatttttt aattgttagt atcggttatg
     8461 ttctttacac tattactagt tgctacttag gtactttaaa tggtatagga aaaccaacaa
     8521 aaagtatgat actaatgata ttctactata ttattattcg tattcctttt gcctatctat
     8581 tatcttggct ggcagaaaat ttaaatggtg tttggatagc gattttaatc agtcacatag
     8641 tcgcctgcat tataactatt ttaacaagta atcaaattat taagcggtta tatggaaaac
     8701 tttatgatag gactaaaaaa gcaagatata aaagcattga aagtaactgc tgtggaaagt
     8761 tttaggaata atcttatacg attatgcact acctaacagc attttatcaa caactaaata
     8821 ttttacaata ttaaaagttc gtacagacgg tatattttgt acggactttt ttcttgttct
     8881 tctttttgta ttttccaaag atatagacgg tatctccgtt tatatatttc agcgtatgcg
     8941 gaaaggaggt gagattatga acccatcttc tttcgagcat atcgttagaa tacagtttaa
     9001 tgctttgatg atgactgtta ttaaatgcac agtaaaaaac agaaacagac agtttgcaag
     9061 acgttctaag cgtgaagttt tattctgtga attatcagat acgaaaaatg ttgaatgtgt
     9121 aactaaggat aactattctt gtgattacat ttcgtttgaa gtcttgaatt ttacaattca
     9181 aatatcaaat gagaaacttg ctgttgcttt atataagtta tcgaataaag agcgtgatgt
     9241 aattctatta cgctattttc aaagtatgag cgaccaagaa atagccgaat tatatcatgt
     9301 atcacgttct gctatttatc gcagaagaag taacggattg aagaaactaa aaacagtatt
     9361 gaaagaaagg aattgaacca atgaaaaaag aatatggcta ccctacatta aaagtaatat
     9421 gtcaagcgtc tgctggaaat gaaactgcca taagagagat tttgaagttt tatgacgctt
     9481 atatatgtaa attatgcttg cgtccgtttt accactctga aagtggtaaa atcattatgc
     9541 aggttgatga agaattaaaa ggtcaaatcc atacagaaat gatgaaagca atcttgaaat
     9601 ttgaaatcag agtaaaataa tcatactaca tgaatgaaaa acgggagata cacagatatt
     9661 tgtgctatct cccatgttgt ttttgtgtca tatcaaggct cattcaatcg tggtaaattc
     9721 gtggtaaaat gaatagtttt ctatacttca aaatgtttga aagtatagtg tttatgcgga
     9781 taatcaaaaa tcagatataa aatttatatc aatttttata aaaaatttgg cttttcacct
     9841 aaagaaatga gtgaaaggta taaacttaat gctagaaatg ttaagataaa tcttaacata
     9901 aaatgtcata aatttttacc ttatgttaaa aatttacaag caagtagtac taacttatgc
     9961 tttaaaataa acacaaatat tgatgagcca aaaaaatact tagaacaaaa tgagattcaa
    10021 attgagcgag gaattgtaga atgtacagga gtctttagta atatgaaatc tatttatttg
    10081 aaacaaacta atggaaattt aattgaatta tcaaatcata gaatcacgga gagtaaacct
    10141 tggaaaattt aatcctatca ataaacgttg ttcttccttt gtttctcaca atgtcacttg
    10201 gatatgttct aaaaaaacta ggtctatttg atactcatac tttaaataaa atgaataatg
    10261 tctgtttcaa gtctttcttg ccattattat tattttttaa tatatacaat acagatttag
    10321 atggttctct taattttaag cttatggctg tagctgttat aattattttt tcaatcttct
    10381 taatcttagt ctttattatt cctaaaattg aaaaagataa taagacaagg ggtgcattaa
    10441 tacaaggtgt atttcgtagc aatttcgtta tatttggtat tcctgtgaca atttcactat
    10501 ttggcgataa gagtattgga gttacatctt tattaattgc agttattgta cctatgttta
    10561 atgtattgtc tgttgtaata cttgaaattt ttagaggtgg caaagtaagt ataaaacata
    10621 tgattaaaag tgtcttaaca aatccactca taatcgcctc ttttatagga ttgacaatgc
    10681 taattttaaa tattaatata cctacatttt tacaaaaaac tattggtgat atttctaaaa
    10741 tagctactcc tttatcactt ataattctag gcggctcttt tagtttttct gctataaagg
    10801 ggcatataaa gcccattata ttgggggtat gtggaaaact tatacttgta cctgctatat
    10861 ttttaccaat cagcattttt cttggattta gaaatgtaga attagcttca cttatgataa
    10921 tgttatctgc tccaactgca gtttcttcat tcacaatggc acaacagatg gattctgaca
    10981 gtgaattagc tggacaaatt gtggtattta cttctgcatt ttctataatt acagtatttt
    11041 taataatatt tattttaaag cagaataatc ttatttaatg atatttgata acacaaattg
    11101 gtattatgaa aaacatatat aaaattgatt tagttgcgta aaaacacatt attatactat
    11161 ttttactata taatataact ttatgtatat tatctcatga aatatggtga aagtactgtt
    11221 gctgtttatt ttttagttta aaaaaatata tagatacaaa caatagatgc taacagatag
    11281 tcaatctata aaataaaaac tggactaatc atataaaatc attagtccag cttttatttt
    11341 acgtactttt tatgttgatg tacttttatt aaaaactaaa tttaattttt aaatgtctag
    11401 caaatgcact aattaaataa tggccagtta ttcaacaata tcactacttc ttaactataa
    11461 gtaatgccaa tgcatttata aaaaataata ctgctgttat ctttgtaact ctagcaagaa
    11521 atgcttgttt tccttttggt ttaaagtaag cttgacttcc ttctccacca aactctgaag
    11581 gtaaagcatt ctttgaatct tgaggcatta tgctaattac tagtaaaatc cctagtacta
    11641 cttgcacacc cattaaaatg ttgctcattc ttgcccctcc tatctgttta ttacttaaat
    11701 ttcattactc tttagtgtat cacaataata tagccatttc aagactatta tgctataaat
    11761 cacatataaa aaaatatata attactgtat cttttttgag taacgtattt ctaataattt
    11821 tttcccaata tatatctctt tctcaaaatc atcacaagta aaaccatttt ttttgtaaaa
    11881 ttttatagct ctagtattat cttttaagac ccatattgtt atatacgaat atcctaaatt
    11941 tcttagtcta tcaatacaaa attgtaatcc tttttttgta acaggagttc cccaaaaatc
    12001 tggatggaaa tatatagagt atatttctcc aatatatttt ggagcatttt cttcatgact
    12061 tttatttaat gaagcaaaac cagcaggtat accatccact ttaaaaagat agtattcctc
    12121 ttcagctttt ggaataacat tgtgaaaaat ttctgctctt ttttcagaag tgaactcatc
    12181 tattatatta tctggaataa ttcctttata cgcttttttc catgaataag aatggataaa
    12241 acccatttct gtagcatttt cacctgtagc tcgtataatc tctaaattca taaaaaccac
    12301 catcctattg acaaattaat acgactatcg tattatatta gtacgataat cgtatcataa
    12361 ttggaggtaa atgtcaatga atgctataaa agaactaagc aacctatggc acatcgttgg
    12421 tatgcaaatg aaaacagaaa tgtgtagtgg taagtatcct actatttatg gtatttcttt
    12481 agtagaatta agtattttag aaatagttga acaacaccca aatagtatga tgaaagaaat
    12541 tagttcaact ttagaattac caaaaagcac attaacaagt gctgtaaaac gtcttgaatt
    12601 aaaaaaactt ataaagagaa atccttgcat taaagataaa agggcgtata atttagattt
    12661 aactgaatta ggtttaaaag ctcagcaaga acatagagaa atagaagcaa ctgtatttaa
    12721 aaatatccta catcaattag ataaagatga gatagaaaca tttattcata ttttttctaa
    12781 agctgtgaat aaaattaggt aataaaatat aaaaaggata agtgaattga aaaatcaact
    12841 aacacttatc cttatataag acaaacttaa accttttata ctatctcttt aatcttaata
    12901 actatttttt taagttatag aaagatttta atccacagta tctagcttgc tctccaacca
    12961 tttcttctat tcttaataat tggttgtact tagcaactct atcagttctt gatggagcac
    13021 cagtttttat ttgaccagca tttactgcaa cagctaaatc tgctatagta gaatcttcag
    13081 tctctcctga tctatgagat ataacagcag tatatcctgc tctcttagcc atttctatag
    13141 catctaaagt ttcagttatt gtacctattt ggttaacttt aactaatata gagttagcta
    13201 ctccattttc aattcctttt tctaatctct ctgtgttagt aacaaataag tcgtcaccaa
    13261 ctaattgtaa tttatttcct aatttttcag ttaataattt ccatccatcc caatcttctt
    13321 cgtctaaacc atcttctata gttattattg ggaagttgtt tgaccaatct tcatataaag
    13381 caaccatttc agcagcagtt aactcttttc cttctcctgc taaaacgtat tttttagttt
    13441 ctttgttgta catttctgta gcagcaacgt caagacctaa cataacgtct tcacctggct
    13501 tatatccagc ttttgttata gcttcaacta ttaattctaa agcttctctg tttgaaccta
    13561 agtttggagc aaatccacct tcgtcaccta caccacaagc taatcctttt tctcctaaaa
    13621 ctttctttaa tgaatggaat acttctgctc ccattcttaa accttctttg aaacaacaag
    13681 ctcctactgg taatatcata aattcttgaa catcaacgtt gttatcagca tgctctccac
    13741 cgtttaatat gttcatcatt ggaacaggta attgtttagc atttactcca cctaaatatt
    13801 ggaataatgg aagacctatt tcatcagcag ctgctctagc tactgccatt gaaacaccta
    13861 atatagcatt agctcctaat ttacctttgt ttggagttcc atccaattct atcatgattg
    13921 cgtctatagc tggttggtca aatgcatcca ttccttctat ttcaggtgct attatttcat
    13981 ttacgttagc aacagctgtt tctacacctt ttcctaagta tcttccctta tctccatctc
    14041 ttaattcaac agcttcaaaa gctcctgtag atgctcctga tggaactatt gctcttccca
    14101 ttgctccatc ttctaatact acttcaactt ctacagttgg atttcctctt gagtctaata
    14161 cttctctagc atatactaat tcaataactg acatttgtat atctcccttc gattttctat
    14221 tagttaaatt ttcatttact gtaaacatcc gtctactatt ttaatctact ttctatatta
    14281 tagtacataa agttgatttt gaaaattagg tacaaattta tttgatgggt aaatttgtaa
    14341 aagttcat
//
LOCUS       DAEPXF010000094        14188 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain HB27
            SAMN13376389-rid12479423.denovo.099, whole genome shotgun sequence.
ACCESSION   DAEPXF010000094 DAEPXF010000000
VERSION     DAEPXF010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13376389
            Sequence Read Archive: SRR10518432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 14188)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14188)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001069624.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001069624.1
            Genome Coverage       :: 315x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/15/2021 01:54:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,165
            CDSs (total)                      :: 4,076
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 89
            rRNAs                             :: 10, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 10 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 19 of 69
            Pseudo Genes (incomplete)         :: 44 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 10 of 69
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14188
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13376389-rid12479423.denovo.099"
                     /strain="HB27"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="China: Hebei"
                     /collection_date="2017"
     gene            217..2094
                     /locus_tag="KRL67_002421"
     CDS             217..2094
                     /locus_tag="KRL67_002421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HBH1523293.1"
                     /translation="MDYKNIAQEILLNVGGKENVNEVTHCMTRLRFKVKSASKVNKDK
                     LSKTEGVITVVESMGQIQVVIGNKVKKVYDEVIKIVPQSNEVGKKNESQEKQGIINSI
                     LSAVAGIFTPTIPAIAGVGMIKGILSVLAMYYMNKNGVDIKETQSYIILNAMADSIFY
                     FMPIILGYTAAKVFNANKIISMVLGATLCYPTFTALMAGEESVRFLGLAVTKATYTSS
                     VIPIIIAIWALSYVEKVLEKYIPEVIKIIMVPTLSLVVMLPATLFLFGPIGIYIGNVI
                     NFSYKYIYELSPALCGAFVGGLWCVLVIFGAHRALLPIGISDVAQTGRQNLLAFAGAA
                     NFSQAGAALGVFFKTKNQGLKTISMSATITALFGITEPAIYGANLRLKKPMVCAVICG
                     AVGGAIMGMGGAYGNAFANQGILTIPVYAEAGALGFLSYLGGCAIAFFGSAISTYLVG
                     FEDLEESSNKENSSIKIETKDGVIDITSPVEGECIELSEVKDDVFASKAMGEGIAVLP
                     TKGVITAPTDCEVASLFPTLHAIGLRLDNGAEMLIHVGINTVELNGKYFTKHVNQGDL
                     VKKGDKLISFDIDKIKNAGYDVTTPVIVNNTFDFGQVVSCKSSYVSTNDNIISLVLVG
                     N"
     gene            2406..3812
                     /locus_tag="KRL67_002422"
     CDS             2406..3812
                     /locus_tag="KRL67_002422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HBH1523294.1"
                     /translation="MNTGFPKDFLWGASSSAFQVEGAWDKDNKGKTVADYNSFKKSHL
                     QADTKVASDFYHNYEEDIELMKELGMKTYRFSISWARIIPDGEGEINQKGLDFYNKII
                     DKLIECDIEPFITLYHFDLPFKLVEKYNGWESRETVYAFERFAKICFKHFGDRVKYWQ
                     PHNEQNLIVRVEERINIYDETDSWKIDKMRAQMDYNLCLAHALAVNACHEMIKESKIG
                     AAVSSSVTYPLTSKPEDIYAARMNDNFKVYYMLDMHHYGEYPGYYMKYLEKRNIVPHM
                     EDGDKEILKKAKMDFIAVNYYRTNCAEALPEDSQHPFGLREGTVDFSMYGLFKMSMNP
                     NLEASEYGAAIDPSGLRVALNEYWQRYHLPVIITENGLGAKDILEDGKIHDDYRIDYL
                     RSHINACKLAIEDGVEMIGYCPWSFTDLLSSSQGFNKRYGLVYINRTDHEVLDLKRIK
                     KDSFYWYKEVIENNGIVK"
     gene            3840..4688
                     /locus_tag="KRL67_002423"
     CDS             3840..4688
                     /locus_tag="KRL67_002423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HBH1523295.1"
                     /translation="MYITKVLNNSLLLAKDDNDEEIILMGKGIGHNYKAGSELNKKDI
                     EKIFVLHDENIKKSIIQLATEIDAEYFGIAQMIIAYGIEKYNLKLMNHIYLALTDHIA
                     FAVRRFKEGIMIENHYLFEIKEFNPKEYDIGKYAITVFKEVLGLDLPEEEIGNIAGHF
                     INAQQDNPYSDRNKRSAKIVNAILQIVHYHFSIVYNKESFYYRRFVMHLKAFSQRFLA
                     NEPSRERIDFIYEQVQKNCKEEYECVKKVGAYMYKEYSRELPRQEELYLMIHIHKILG
                     ELCENS"
     gene            5350..6042
                     /locus_tag="KRL67_002424"
     CDS             5350..6042
                     /locus_tag="KRL67_002424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003020373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBH1523296.1"
                     /translation="MNKVLIIDDDKDLCILLKKSISIEQIEANYCHSGRDGLVELSKQ
                     DYQLVVLDVMMPGMDGFETMEQIRKYSSIPILMLTSKSDNYSKVHGLRAGADDYLTKP
                     FEIAEFTARVLSLIRRYTRFNPKSEEPQQLSFKGLLIDLDQHTVTTNGEPMLLPPKEF
                     GVLLYCARHQGKILTKQQIYEAVWGEPYVYDDSNIMAIISRLRKKIELDPSSPIYLQT
                     IKGIGYQFNTEV"
     gene            6039..6974
                     /locus_tag="KRL67_002425"
     CDS             6039..6974
                     /locus_tag="KRL67_002425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBH1523297.1"
                     /translation="MKMDEQTIILFLFLLSIGLLLLSIFAFFKLGHIHKRLKDIEEML
                     ADVQIGNENRKILIKPCDVMAPLVYQLNEIVYDYENKLLSLKKSDKASKQLMTSLSHD
                     VRTPLTTLIGYLDAVHSGIVIGQEREEYLEIARRRAYDLKDYIDVLFDWFRLNSDEFT
                     LSIESVEIAELSRNILKDWIPIFHEKKLDFEIDIPENRLMVNLDPDGYSRVVNNLVQN
                     VLTHSKACRIKITISEDSKMVLFRVEDNGVGIAKENLQHIFERLYKCDKGRSKKGSGL
                     GLSIVSQMVERMGGQISVESELGKYTVFTVSLPLI"
     gene            7123..8046
                     /locus_tag="KRL67_002426"
     CDS             7123..8046
                     /locus_tag="KRL67_002426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013273760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBH1523298.1"
                     /translation="MNNLIIETKNLTKQYGAQKSVSNLNIHVQKGRIYGLLGRNGAGK
                     TTTMKMLLGLTKPTTGEIKIFGQDIHNNEKKLLPRIGCLIESPGFYPNLTGTENLKIF
                     ARLRGIPKKNAVESALEVVGLPNNDKKLFSQYSLGMKQRLAIALAIMHDPQLLILDEP
                     INGLDPIGIAEVRKFIRDLCTERGKTILISSHILSEISLLADDIGIIDQGILLEEESL
                     AQLEEKNSKYIHFAVSDAAQAARLLEIQLNLKNFKVADNHNIMLYDTDVSVAEINRAF
                     IQNNIDVSESHLCEDTLEDYFKKVTGGEGIA"
     gene            8030..8800
                     /locus_tag="KRL67_002427"
     CDS             8030..8800
                     /locus_tag="KRL67_002427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBH1523299.1"
                     /translation="MKELLDIISCEFSKLKRLKFILISILGACLFPIPATILIAKDNL
                     PFEQLFKLVVNFGYFLLLPIVLSIVASQLFFIERDNDVLKNLATVPVPKGKLALAKLA
                     VLLFISLFYSVAGLGATIIGGFIVGIVEGVAVKLGMSIAFGIMFFVTALPVVVLIVYF
                     NRSYIFSIILSFVYAIFNFSISLNIINFKPNNLLLSILPAPVIMRWWMAFWGDPTGEY
                     TALRQPYLLSTPACVGILCLITIIAVLLICMIYKKQEN"
     gene            8810..9592
                     /locus_tag="KRL67_002428"
     CDS             8810..9592
                     /locus_tag="KRL67_002428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBH1523300.1"
                     /translation="MLDIITTEFQKIKRYNILWVGVVAVLFSALLAVFQIVSSHGSDP
                     LTYESFANGVIWNNFSLSFPFGITLIGGYLINREYTEQTLKNILTVPISLRKLLVGKL
                     IAVGGITVLLSLFSFLCTFILGLLFCNINISVTLVVKSLVQILSVNLYCYVAVLPIIA
                     YFGRKQNAFLTGVGIAFVYGFCGVFVAGRNLTDFYPITAGLGLVGYNNGAGTVYQPLI
                     GAMTLIVILILTTILLVFTPNYDKVMTISKKKGTKHKKYSHK"
     gene            9969..10505
                     /locus_tag="KRL67_002429"
     CDS             9969..10505
                     /locus_tag="KRL67_002429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubrerythrin family protein"
                     /protein_id="HBH1523301.1"
                     /translation="MNLKGTKTEKNLMAGFAGESEARNKYTYYASKARKEGYNQIAAI
                     FEETANNEKEHAKLWFKLLHDGMPSTEENLKDAAAGENYEWTDMYAKFAKEAREEGFD
                     KIAYLFEAVGKIEKEHEERYLKLLENLNEGKIFKRDEEVVWQCQNCGHVYVGTEAPEK
                     CPVCDHPKAYFNIKAENY"
     gene            10536..10925
                     /locus_tag="KRL67_002430"
     CDS             10536..10925
                     /locus_tag="KRL67_002430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HBH1523302.1"
                     /translation="MKFSKQRELILNEILNNPVHPTADYLYENLKKDNPNLSLGTVYR
                     NLAQLTEHGFIRKVSIPGNPDRFDGRIDNHYHIICEVCGEIYDLESEVLNNLQELISE
                     ETDIKITSYNISFKGICKNCKKCSQVG"
     gene            10962..11348
                     /locus_tag="KRL67_002431"
     CDS             10962..11348
                     /locus_tag="KRL67_002431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="desulfoferrodoxin"
                     /protein_id="HBH1523303.1"
                     /translation="MCSEQKFFICETCGNLVGMIQSGGVPIFCCGKPMKELVPNTTDA
                     AVEKHVPVIEVDGNNVTVKVSSTTHPMTKEHHIAWVYLMTEQGGQRKCLAVDGEPVVK
                     FALNDDDKVISAYAYCNLHGLWKAEL"
     gene            11475..12917
                     /locus_tag="KRL67_002432"
     CDS             11475..12917
                     /locus_tag="KRL67_002432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HBH1523304.1"
                     /translation="MSIYKCSVCGYIYDESKNDKTWDELSEDWECPVCTKGRSYFGKI
                     STVYYEEDEKVAEDIVEDESKLNTEKEGDLNYLSTYLRRDDEVEKHMDIIHEMAVTGK
                     SIIEPMRTKLPVISWDDILIMGAQLNPLPLNEHDEVNTTTIIGKKAKKPMIIENPVYI
                     SHMSFGALSKELKIALAKGAAQNKTAMCSGEGGILPEEKEASYKYIFEYVPNKYSVTE
                     ENLKNSDAIEIKIGQGTKPGMGGHLPGEKVTEEIAKVRNKPVGQDVISPSCFEEIQSK
                     EDLKKLVDELREVSEGRPIGVKISAGHIEKDMEFIAYAKPDFVTIDGRGGATGASPKL
                     LKDATSIPTIFALYRARKYIDTHGLDMDLVITGGLRISTDFAKAIAMGADAVAIASSA
                     LMAAACQQYRICGSGKCPVGVATQDEELRKRLHIENSANRVANFLNVSLEELKTFARI
                     SGHKDIHDLSVDDLYTVNSEISNYTNIQHV"
     gene            13215..14030
                     /locus_tag="KRL67_002433"
     CDS             13215..14030
                     /locus_tag="KRL67_002433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HBH1523305.1"
                     /translation="MERLKIQVLVDNNTYIDRYFVGEPAVSYYIEIDGNRILFDSGYS
                     DVFISNAEKLNIDLGNLTHVVFSHGHNDHTRGIQFLENRYDLSTVELISHPNCLIPKK
                     HGEKSIGAPFSAEEIKNIFMYNPKDKPFNLSKNCVFLGEIPSINNFEKRAKIGKCKIG
                     DLWEDDYVLDDSAIVCKTDKGIFIVTGCSHSGICNIVEYAKKVCGDDRVIGILGGFHL
                     FELNNRLDSTIQYLDKEEIRMLYPCHCVSFKVKAKMNEILNINEVGVGMTISI"
BASE COUNT         5127 a         1723 c         2694 g         4644 t
ORIGIN      
        1 ctttatagtt gaattaagaa aacgcttatg atactataaa gttaacaaat ttgaatatat
       61 aacagtagac gtgttactgg taatgcaggc aagatcgaat caatttttat agattatcta
      121 tagagtacta tgggtataat ctatttattt ggttcgattt ttttgtttaa ttggccataa
      181 aacaaaaagc ctatagttga aaatggagga ttttttatgg attataaaaa tatagctcaa
      241 gaaattctac taaatgtagg aggaaaagag aatgtaaatg aagtgactca ttgtatgaca
      301 agacttagat ttaaagtcaa aagcgcttct aaagtgaata aggataaatt atctaaaaca
      361 gaaggagtaa taactgttgt tgagagtatg ggtcaaatac aagttgtaat tggaaataaa
      421 gtaaaaaaag tatatgatga ggttataaaa attgtacctc aatcaaatga agtaggtaaa
      481 aagaatgaaa gtcaagaaaa acaaggtata attaattcta tattatcagc agtagctggt
      541 atatttacac ctacaatacc tgctatagca ggggttggta tgattaaagg tattttatca
      601 gttcttgcta tgtattatat gaataaaaat ggagtagata taaaagaaac acaaagctat
      661 attatcttaa atgccatggc cgattctata ttttatttta tgcctattat actaggatat
      721 actgcggcta aagtatttaa tgcaaataaa ataatatcta tggtattagg agctacactt
      781 tgttatccaa cttttactgc tttgatggca ggggaagaat ctgttagatt tttaggattg
      841 gcagtaacaa aagctacgta tacatcatct gttattccaa taattattgc tatatgggct
      901 ctttcatacg ttgaaaaagt attggaaaag tatatacctg aggtaattaa aataataatg
      961 gttccaactt tatcattggt tgtaatgtta cctgcaacat tgtttttatt tggaccaatc
     1021 ggaatttata taggaaatgt cataaacttt tcttataagt atatttatga attaagtcca
     1081 gcattgtgtg gagcatttgt aggtgggtta tggtgtgtgt tggttatatt tggtgctcat
     1141 agagcactat taccaatagg tataagtgat gttgctcaaa caggaagaca gaatttattg
     1201 gcatttgcag gagctgcaaa cttttcacaa gcaggggctg ctctaggagt attctttaaa
     1261 accaaaaatc aaggactaaa aacaatttct atgtctgcaa ctataacagc tttatttggt
     1321 ataactgaac ctgcaatata cggtgccaat cttagattaa aaaaacctat ggtatgtgcc
     1381 gtaatatgtg gtgcagttgg aggtgccata atgggaatgg gtggagctta tggtaatgca
     1441 tttgcaaacc aaggtatttt aactataccc gtatatgctg aagcaggtgc attaggtttc
     1501 ttaagttatc taggtggttg tgctatagca ttttttggtt cagctatttc aacttatcta
     1561 gttggatttg aagatttgga agagtcttct aataaggaaa attcatctat aaaaatagaa
     1621 acgaaggatg gtgttattga tataacttca cctgtagagg gagaatgtat agaattatca
     1681 gaagtaaagg atgacgtatt tgcttcaaaa gctatgggag aaggtatagc tgtgttacct
     1741 acaaaaggag taataacagc tcctactgat tgtgaagtag cttctttatt tccaacatta
     1801 catgcaatag gattaagatt agataatgga gcagaaatgt taatacatgt tggaatcaat
     1861 acagtcgagt taaatggtaa atactttaca aaacatgtta atcaagggga tttagtgaaa
     1921 aaaggtgata aacttatatc ctttgatata gataaaataa aaaatgctgg ttatgatgta
     1981 actacgccag ttatagtaaa caatacattt gattttgggc aagtagtttc ttgtaaatct
     2041 agttatgttt caaccaatga taatataatt tcattagtat tagttggtaa ttaagttaat
     2101 ttgactagag ataagtaaat tgatttaaaa ggaaataagt aaatcaacaa ttaaatacat
     2161 ctaaaagtaa gtttattgca atgaattgtt aaagaaaata attaatatgg agtaaagcta
     2221 ttgcaatgaa gtaacaaaga aaataattaa tatagagtaa gtttattgta atgaattgtt
     2281 aaagaaaata attaatatag ggtaaatcta tctcaatgaa ataacaaaga aaagagttaa
     2341 tatagagtga acttattgca atgaatcaac aaagaaaata atcaatataa aaaggagaaa
     2401 tatttatgaa tacaggattt ccaaaagact ttttatgggg agcatcatct tctgcatttc
     2461 aagtggaagg agcttgggat aaagacaata aaggaaaaac cgtagccgac tataattctt
     2521 ttaaaaagtc tcatttacag gcagatacta aagtcgcatc agacttttac cataattatg
     2581 aagaagatat tgaattaatg aaagaattag ggatgaaaac atatcgtttt tcaatttcat
     2641 gggcaaggat aataccagat ggtgaaggag agataaacca gaaaggttta gacttttata
     2701 ataagataat agacaaacta atagagtgtg atattgagcc attcataact ttataccatt
     2761 ttgaccttcc atttaaatta gtagagaaat ataatggttg ggaaagtaga gaaactgtct
     2821 atgcttttga aagatttgct aaaatatgct ttaagcattt tggagatagg gtaaagtatt
     2881 ggcaacctca taatgaacag aatttaattg ttagagtgga ggagagaatt aatatatatg
     2941 atgaaacaga ttcatggaag atagataaaa tgcgtgctca gatggattat aatctttgct
     3001 tagcacatgc attagcagtc aatgcatgtc atgagatgat aaaagaaagt aaaattggag
     3061 cagcagtatc atcatctgta acatatcctc ttacttctaa gccagaagat atatatgcag
     3121 ctagaatgaa tgataatttt aaagtgtact atatgcttga tatgcatcat tatggagaat
     3181 atccagggta ttatatgaag tatcttgaaa aaagaaatat agttcctcac atggaagatg
     3241 gggataaaga gatacttaaa aaagctaaaa tggattttat agctgtaaat tattatagga
     3301 caaattgtgc agaagcttta cctgaagatt cacaacatcc atttggatta agagaaggaa
     3361 ctgttgattt tagtatgtat ggactattta aaatgtcaat gaatcctaac ttagaggcct
     3421 ctgagtatgg agcagcaata gacccatcag gtcttagagt tgctttaaat gaatattggc
     3481 aaagatatca cttacctgtt ataataacag aaaatggtct aggagctaaa gatatattag
     3541 aagatggaaa aattcatgat gactatcgta tagactattt aagaagtcat ataaatgcat
     3601 gtaaactagc tattgaagat ggtgtagaga tgattggata ttgtccatgg tcatttacag
     3661 accttttaag ttcttctcaa ggttttaata agcgttatgg acttgtttat ataaatagaa
     3721 ctgaccatga agttttagat ttaaaaagaa tcaagaaaga tagtttttac tggtataaag
     3781 aagttataga gaacaatgga atagtaaaat aaacataaat atatattgag ggttttagta
     3841 tgtacattac aaaggtatta aataattcgt tgctacttgc aaaggatgat aatgatgaag
     3901 aaataatatt gatggggaaa ggcattggcc ataattataa ggccgggtct gagttaaata
     3961 aaaaagatat agaaaagatt tttgttcttc atgatgaaaa tataaaaaag agtattatac
     4021 aattggcaac agaaatagat gcagaatatt ttggtatagc tcaaatgata attgcatatg
     4081 gaatagaaaa atataactta aaacttatga accatatata tttggcctta actgaccaca
     4141 ttgcctttgc agtgagacgt tttaaagaag gtataatgat tgaaaatcac tatttgtttg
     4201 aaatcaagga gtttaatcca aaagagtatg atataggaaa atatgcaatt actgttttta
     4261 aggaggttct tggtttagac ctaccagaag aagaaattgg taatatagca ggccatttta
     4321 taaatgcaca acaagataat ccttatagtg atagaaataa gagaagtgct aaaattgtaa
     4381 atgctatttt gcaaatagtt cattatcact tttctattgt atataataaa gagtcatttt
     4441 attataggag atttgtaatg catctaaagg ccttttcaca aagatttctg gctaatgaac
     4501 catcaagaga aagaatagat tttatatatg agcaagttca aaaaaattgt aaggaagagt
     4561 atgaatgtgt gaaaaaggta ggagcttata tgtataagga atactccaga gaattaccaa
     4621 gacaagagga gttatatttg atgattcata ttcataagat tttaggagaa ttatgcgaaa
     4681 atagttagaa tatcttaata tgctagtaat attataaaaa attaaaaatt aactacactt
     4741 taaatatgaa taattgcact acttgaggga gttgtcttaa aattgatttt tttaattaaa
     4801 tttgaagctc aactcctttt ttaaaccttt atatcattac gactttaatg tttatacgcc
     4861 tgtacttcag atatatatct actttttaca tttctcaaca tgcaaagcta tcattttttg
     4921 cagcatgtta attgaaataa acctttgagt taacgctaaa tttattctgg acttacagga
     4981 atagttccaa agatacttta aaatattttc tggaatgatt gcttgtttca tatatatttt
     5041 attgtgatgt tttgatttaa taaattcttc aactctgtat atagtaaatg tattatcatg
     5101 tttgttttat tcaagcacac ttataatacg cttgattttt tcacaatgat gaaatatact
     5161 aatgggtgtt gtagatataa gaatcacatt acacttgaaa aagtaagtga gaaatgaggt
     5221 ataactcctc atatgataaa ctactatttc attgtggaac atattctaag tgatgtcaag
     5281 atactacaat atgactaaat tcctaatgat aaaaaagtct acgaatgagt gtagaaaggg
     5341 gggagtaaga tgaataaagt tttaattata gatgatgata aagatctttg tattttgtta
     5401 aaaaagagta tctcaattga acagattgaa gcaaactatt gccattctgg gagagatgga
     5461 ctggttgaac tttcaaaaca agattatcag ttagtagttc tagacgtgat gatgccaggt
     5521 atggatggtt ttgaaactat ggaacaaata cgaaaatata gcagtattcc catcttaatg
     5581 ctgacatcga aaagcgataa ttacagcaaa gtgcatggat tgcgagctgg tgcagatgac
     5641 tatctaacca aaccatttga aattgcagaa tttactgctc gtgtactgtc tttaatccga
     5701 cgttacaccc gttttaatcc aaagagtgag gagccacagc aactttcttt caaaggtttg
     5761 ttgattgacc ttgaccagca tacagtgact accaatggcg aacctatgtt gcttccaccc
     5821 aaggaatttg gtgtgcttct ttactgtgca aggcatcagg gtaagatttt gacaaaacaa
     5881 cagatttatg aggccgtgtg gggtgagcca tatgtatatg atgacagcaa tatcatggct
     5941 attatcagtc gcctaagaaa aaaaattgaa ctagacccat cctctcctat ctatttgcaa
     6001 acgataaaag gaatcgggta tcaattcaac acggaggtat gaaaatggat gaacaaacga
     6061 taattctttt tttgttcttg ctgtcgattg gtctgcttct cctttccata ttcgcctttt
     6121 tcaagcttgg gcatattcac aaacgtctaa aagatataga agaaatgctt gctgatgtac
     6181 aaattggaaa cgaaaatcgt aaaatactaa ttaagccatg tgatgttatg gctccattgg
     6241 tttatcagct taatgagatt gtttacgatt atgaaaataa gttactttct ctgaaaaagt
     6301 cggataaagc tagcaagcaa cttatgacaa gtttatctca tgatgtccgt acaccattaa
     6361 caactctgat tggttatcta gatgctgtac acagtggtat agttatcgga caagaacggg
     6421 aggaatacct tgaaatcgct aggcgtagag cctacgacct caaggattat atcgatgttc
     6481 tttttgactg gtttcggttg aactctgatg aatttacgct gtcaatagaa tctgtggaaa
     6541 ttgcagagtt gtcacgcaat attcttaaag attggattcc tatcttccat gaaaagaaat
     6601 tggattttga aattgatatt ccagaaaatc gactaatggt aaaccttgat ccagatggat
     6661 attctcgtgt agtcaataat ctggttcaaa acgttcttac tcacagtaag gcttgtcgaa
     6721 tcaagattac tataagtgag gattctaaga tggtattatt tcgtgttgag gataatggtg
     6781 ttgggattgc caaagaaaat ttacaacata tttttgagcg actgtacaag tgtgataaag
     6841 gacgttcaaa aaaaggcagt ggtcttggcc tatctattgt cagtcagatg gttgagcgaa
     6901 tgggtggtca gatttctgta gaaagtgaac ttggaaagta tactgtattt acagtgagct
     6961 taccattaat ataaagattg tttttccccc atgtgacata tataaacagg gggatttttt
     7021 ataagctata aagttgcaag gttaatacaa ggttgttgca aggttaatgt aagattgagg
     7081 tggtatgata actttaataa acaaaggagg attttactat ttatgaataa tttaattatt
     7141 gaaacaaaaa atctcactaa acaatatggt gctcaaaaga gcgtttctaa tttgaatatt
     7201 catgttcaga aaggtcgtat ttatggattg ctgggaagaa atggtgcggg taagacgaca
     7261 actatgaaaa tgttattggg tttaacaaaa ccaactactg gagaaattaa aatatttggt
     7321 caggacattc ataataacga gaagaagctt cttcctagaa ttggatgttt gattgaatca
     7381 ccaggttttt atcctaatct gacaggaaca gaaaatctca aaatctttgc aaggcttaga
     7441 ggaataccaa aaaaaaatgc agtagaatca gcactggagg tagtcggttt gccaaacaat
     7501 gacaaaaagt tattttctca atactctctt ggcatgaaac agaggctggc gattgctctt
     7561 gccattatgc atgacccaca attgttgatt ttggacgagc ctataaatgg acttgatcct
     7621 attggtattg cagaagtaag gaagtttatt cgtgatctct gcacagaacg aggtaaaact
     7681 attttgattt ccagtcacat tctttctgaa atatcattgt tggcagatga catcggtatt
     7741 attgaccagg gcattttgtt ggaggaagaa agccttgcac aactggaaga aaaaaacagt
     7801 aaatacatac attttgctgt atctgatgca gcacaggcag caaggcttct ggaaatccaa
     7861 cttaacctga aaaatttcaa agtagcagac aatcacaata ttatgcttta tgatacagat
     7921 gtttctgttg cagaaataaa tcgtgctttc attcaaaaca atattgatgt atcagaatct
     7981 catttgtgtg aagatactct tgaagattac ttcaagaaag taacaggggg tgaaggaatt
     8041 gcttgatata atttcatgcg aattttcaaa actcaagcgg ttaaaattta tactgatttc
     8101 tattttaggt gcttgcttat ttccaattcc cgcaacaatt ttgattgcaa aggacaactt
     8161 gccctttgag caattgttta aattagttgt caattttgga tattttttgc tattaccaat
     8221 tgtattgagt attgttgcta gccaactatt tttcatagaa agagataacg atgtattaaa
     8281 aaatcttgca acagtgccag tgccaaaagg aaaactggct ctggcaaaat tagctgtgtt
     8341 gctgtttata tcattgttct actccgttgc agggttagga gctactatca ttggaggttt
     8401 cattgttgga atagtagagg gggttgctgt aaaattagga atgagcattg cttttggaat
     8461 tatgtttttt gttactgcac ttcctgttgt ggtactcatt gtatatttca atcgcagcta
     8521 tattttttct attatacttt cttttgtata tgctattttc aattttagta tatccttaaa
     8581 tattatcaat tttaaaccaa ataacctact attaagcata ttacctgctc ctgttataat
     8641 gcgttggtgg atggcatttt ggggagatcc cactggcgaa tacacggcac ttagacagcc
     8701 atatttgctg tcaactcctg cttgtgtagg tattttatgt ttgataacca ttatagcagt
     8761 cctgctgatt tgtatgatat acaaaaagca ggagaattag gaggtatcta tgcttgatat
     8821 aattactaca gaatttcaaa aaataaaaag atataatatt ctatgggttg gtgttgttgc
     8881 tgttctgttt tcagcgctat tggcggtttt ccagattgta agttcacacg gtagtgaccc
     8941 tctaacgtat gagagctttg caaacggagt tatctggaat aatttcagtc ttagttttcc
     9001 atttgggatt acacttattg gaggatatct tattaaccgt gaatatacag aacaaacttt
     9061 aaaaaacatc ctaactgttc ctatttcatt acgcaaattg ctagtgggaa aattaattgc
     9121 ggttggagga attactgtat tgttaagtct tttcagcttc ttatgcacat ttattttagg
     9181 gttgctattt tgcaatatca atatatctgt tacactcgtt gtaaaatcct tagtccaaat
     9241 tttgtctgtt aatctgtatt gttatgttgc tgttttgccg attattgctt attttggtcg
     9301 taaacaaaat gcatttctga caggtgtagg gattgcattt gtatacggtt tttgtggtgt
     9361 gtttgttgct ggaagaaatc tgacagactt ttatccaatc acagcaggtc ttggtctagt
     9421 tggatacaac aatggtgctg gtactgttta tcagccactt atcggtgcta tgactttgat
     9481 tgtaattcta atcttgacaa ctattctact tgtatttaca cctaattacg ataaagttat
     9541 gactatatct aagaaaaaag ggacaaagca taaaaagtat tctcacaaat aatattaata
     9601 ttgtttttat tatttaagtt gccagacagt gaaaagaaaa aatctgtcgt acagtggtta
     9661 tatgttcatg tattcaagaa gctacagttt tgatttttca tagatgtagc ttctttttat
     9721 atttattaaa ttacgactat cacaatattt acaataggta tagtaacaag agagaatact
     9781 gtcatatgtg ttttatatat tacattaagt atattctaaa atatatgctt taaataatat
     9841 atttgaaaat aggaatattt aaataaaaaa aataatatta tatatagttg acaaaaactg
     9901 gaaaatgata tactatataa atatagaaat agtaatcatt actattatta aaaagggagg
     9961 aattaattat gaacttaaaa ggaactaaaa cagaaaagaa tttaatggca ggatttgcag
    10021 gagagtcaga agcaagaaat aaatatacat actacgcttc aaaagctaga aaagaaggtt
    10081 ataatcaaat agctgctata tttgaagaaa ctgctaacaa tgaaaaggaa catgctaaat
    10141 tatggtttaa acttttacat gatggtatgc cttcaacaga ggaaaattta aaggatgcag
    10201 cagcaggtga aaactatgaa tggactgata tgtatgcaaa atttgctaaa gaagctagag
    10261 aagaaggatt tgacaagata gcttatttat ttgaagctgt tggaaaaata gagaaagagc
    10321 atgaagaaag atacttaaag cttttagaaa acttaaatga aggaaaaata tttaaaagag
    10381 atgaagaagt tgtttggcaa tgccaaaact gtggtcatgt gtatgtaggt actgaagcac
    10441 cagaaaaatg cccagtatgt gaccatccaa aagcatactt taatataaaa gctgaaaact
    10501 attaaaaatt attataatta aaaggaatgt taactatgaa attttctaaa caacgagaac
    10561 tgattttgaa tgaaatatta aataatccag ttcatcctac tgctgattac ctatatgaaa
    10621 acttaaaaaa agataatcca aatttaagct taggaactgt gtatagaaat ttagctcagc
    10681 taacggaaca tggctttata agaaaggtta gtattccagg gaatccagat agatttgatg
    10741 gtagaataga taatcactat catatcatat gtgaagtatg tggtgagata tatgatttag
    10801 aatctgaagt tcttaataat ttacaagaat taatatctga ggagacagac ataaaaataa
    10861 catcttataa cataagtttt aaagggattt gtaagaattg taaaaagtgt agccaagtag
    10921 gttagttttc aaattctaaa aataattaat gaggtgagtt tatgtgtagt gaacaaaaat
    10981 tttttatatg tgagacttgt ggaaaccttg taggaatgat acaaagtggt ggtgtaccta
    11041 ttttctgttg tggaaaacct atgaaggaac ttgttcctaa tacaacagat gcagcagttg
    11101 aaaaacatgt acctgtgatt gaagtggatg gaaataatgt tactgttaaa gtaagtagca
    11161 caactcatcc aatgactaaa gaacatcata tagcatgggt gtatcttatg actgaacaag
    11221 gtggtcaacg caaatgtcta gcagtagatg gagaacctgt agttaaattt gctttgaatg
    11281 atgatgataa agtgatttct gcctatgctt actgtaattt acatggatta tggaaggctg
    11341 aactgtaata tttaaataaa ttgaatcaaa aaggaggcta taaatgtatt ttcatacaaa
    11401 tagcctccta taaatttata taaattcaag ttttaatttt gtttaatgtc aatataatat
    11461 aaggagaaaa taatatgtca atttataaat gtagtgtttg tggatatatt tatgatgaat
    11521 ctaagaatga taaaacatgg gatgaattaa gtgaagattg ggaatgccct gtatgtacaa
    11581 aaggtcgtag ttattttgga aagataagta ctgtttacta tgaagaagat gaaaaggtag
    11641 cagaagacat agttgaagat gaatctaaat taaatactga aaaagaggga gatttaaatt
    11701 acttaagtac atatttaaga agagatgatg aagttgagaa acatatggat ataattcacg
    11761 aaatggctgt tacaggaaaa tctataatag aacctatgag aactaaacta ccagtgattt
    11821 cttgggatga tattttaatt atgggtgctc aattaaatcc attaccttta aatgagcatg
    11881 atgaagtaaa tacaacgaca ataataggaa aaaaggcgaa gaaacctatg attatagaaa
    11941 atccagttta tatttcccac atgtcatttg gagctttgtc taaagaacta aaaatagctt
    12001 tagcaaaagg tgcagcccaa aataagacag ctatgtgtag tggtgaagga ggtatattac
    12061 cagaagagaa ggaagcttca tacaagtata tttttgagta tgtaccaaat aaatatagtg
    12121 taactgaaga aaatcttaaa aattctgatg ctatagagat aaaaattggt caaggaacaa
    12181 aacctggtat gggaggtcat cttcctggcg aaaaagtaac tgaagaaata gcaaaagtaa
    12241 gaaataagcc tgtaggtcaa gatgtaataa gtccatcttg ttttgaggaa atacaatcca
    12301 aagaagattt aaagaaactt gtggatgaac ttagagaagt ttcagaaggg cgtccaatag
    12361 gtgttaaaat ttcagcaggt catattgaaa aagatatgga atttatagct tatgctaaac
    12421 ctgattttgt aactattgat ggtcgtggtg gagctactgg agcaagtcca aagttgctta
    12481 aagatgcaac ttcaattcca acaatatttg ctctttatcg tgctagaaaa tacatagata
    12541 cacatggatt agatatggat ttggttataa caggtgggct tcgtatatct actgattttg
    12601 ctaaggcaat tgctatgggg gctgatgcag ttgctattgc aagttctgct cttatggcag
    12661 cggcgtgtca acaatatcgt atatgtggtt ctggtaagtg tcctgtagga gtagctactc
    12721 aggatgagga attgagaaaa agattacata tagaaaatag tgctaataga gtggcaaact
    12781 ttttaaatgt aagtttagaa gaacttaaga cctttgctag aatttctggt cataaagata
    12841 tacatgattt aagtgttgat gatttatata ctgttaactc tgaaatatca aattatacaa
    12901 acatacagca tgtatagatt gaagtttgaa actattagat tgtagatagt tataaaagta
    12961 aatgataaat atattttata aaaaggagtg tcttaaaatg aactttttag tttataagtc
    13021 actctttttt gtatgaagca aaatatattt atcattttaa caatgaaaaa gaatcttatc
    13081 tctttacttt gagatagact ccttctattt atgaaaatta aaagatatta aattgtaaaa
    13141 tattctgtga tgtcgtacta ttaaactaaa gatgctgtat tttaaagtaa ataaaaattt
    13201 aaggagtgat tattatggaa agattgaaga tacaagttct agttgacaat aatacatata
    13261 ttgataggta ctttgttgga gaaccagcag taagctatta cattgagatt gatggaaata
    13321 ggattttgtt tgattctgga tattcagatg tattcatatc aaacgctgaa aaattaaata
    13381 tagatttagg aaatttaaca catgttgttt tttctcatgg tcataatgac catacaagag
    13441 gtatacagtt tttagaaaat agatatgatt tgtctacagt tgaacttatt tcacatccta
    13501 actgtcttat acctaaaaaa catggagaga aaagcatagg agctccattt tctgctgagg
    13561 aaattaaaaa tatttttatg tataatccta aggataagcc atttaatcta agtaagaatt
    13621 gtgtgttttt aggtgaaata ccttctataa ataattttga aaaaagagct aaaattggta
    13681 aatgtaaaat aggagatttg tgggaagatg attatgtttt agatgattct gctatagttt
    13741 gtaaaactga taaaggtatt tttattgtta caggatgttc tcatagtgga atttgtaata
    13801 tagtagagta tgctaaaaag gtatgtggag atgatagagt tattggtata ttgggtggtt
    13861 ttcatctttt tgaattgaat aataggttag atagcacaat acaatatttg gataaagaag
    13921 aaataagaat gttgtaccct tgtcactgtg tttcttttaa agtaaaagct aagatgaatg
    13981 aaatattgaa tataaatgaa gttggagtag ggatgactat aagtatatag tatttgaaag
    14041 tatataagat ttaagttgaa ggttaaaaag atattttcta tagaaagtat cttttttatt
    14101 atgtttattt taagagaaat caatgtactt ttatatgaaa tattgtaaaa aaatttattt
    14161 ttaatgagaa tatattttca ttaataaa
//
LOCUS       DAEPXF010000095        14102 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain HB27
            SAMN13376389-rid12479423.denovo.100, whole genome shotgun sequence.
ACCESSION   DAEPXF010000095 DAEPXF010000000
VERSION     DAEPXF010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13376389
            Sequence Read Archive: SRR10518432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 14102)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14102)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001069624.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001069624.1
            Genome Coverage       :: 315x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/15/2021 01:54:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,165
            CDSs (total)                      :: 4,076
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 89
            rRNAs                             :: 10, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 10 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 19 of 69
            Pseudo Genes (incomplete)         :: 44 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 10 of 69
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14102
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13376389-rid12479423.denovo.100"
                     /strain="HB27"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="China: Hebei"
                     /collection_date="2017"
     gene            112..1044
                     /locus_tag="KRL67_002434"
     CDS             112..1044
                     /locus_tag="KRL67_002434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDT family transporter"
                     /protein_id="HBH1523306.1"
                     /translation="MNLLKKYPIPIASLILSIFTLGNLLQSYSESLRNVIGFIGFILY
                     AIYIVKIILLRKGVKEQLENPLISSTFPTITMATMIFATYVKPLSLELSIMIWSIGIV
                     GHLVLIVLFSKKFLVKFSIKTVFPSWYIVYVGIVAASVTAPAFNQLLIGKIAFWIGFV
                     SYIVLIPVVLKRVWIIKEMPEPSLPSIAILAAPGSLLLAGYINSFSDKNNVVLYLLFI
                     LSIVFYIIVLGYLPKLLRLPFYPSFAAFTFPMAISAMGMKLMNGYLTKSGHFISWISY
                     ASKLEEIIATVIILYVLVLFIIYLSKSETTSKAK"
     gene            1149..1616
                     /locus_tag="KRL67_002435"
     CDS             1149..1616
                     /locus_tag="KRL67_002435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C_GCAxxG_C_C family protein"
                     /protein_id="HBH1523307.1"
                     /translation="MKTKEEYLKEVRNISEEYFRKGEFFCSEAVLQTINDALGQPLSP
                     EITKLASGFPIGLGKAQCLCGAISGGEMALGIVYGRVHGETMNPKMFEYAKGLHDYIK
                     KEYGATCCRAITKKWDGDNFMSPERKEHCIKITGQVTEWVADKLIEDEKLNIK"
     gene            complement(1860..2714)
                     /locus_tag="KRL67_002436"
     CDS             complement(1860..2714)
                     /locus_tag="KRL67_002436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009912760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar uptake protein"
                     /protein_id="HBH1523308.1"
                     /translation="MLTIIIALLPVMGWGLMPIVANLKKSSPHEQLLGTSISAFIFST
                     IITLVIHPEITFFSFCISMVSGIFWSLGQLMQFKAIQIASVSKVMPISNGSQLTFTTL
                     LAVILFNEWTSFKMLLIGSLSILCIILGILLTNYQTKKSTDKNMLKCVGVVLLSSLFL
                     SLYVVTNQIFLIEGYRIILPQSVGMLITSSIIVKYFSKEIVYRENVLFNLITGILWSI
                     ANLGMFITTNTFGVTISFSISQSCVIVATLGGIIFFKEKKNKKEWLAILIGIILIMSG
                     VFMLSIIK"
     gene            2880..4844
                     /locus_tag="KRL67_002437"
     CDS             2880..4844
                     /locus_tag="KRL67_002437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HBH1523309.1"
                     /translation="MLTDMIQNREIKIIKFLLKEGHTTVKAIADDLDVSEKTVSKSLK
                     EVEAVLKKIGLSLIRKPKIGVYIDGDLKKILPYLDNKGSQIPTTREERVIYIYSVLLK
                     RNDYITIQQLSDELYISRGTIERDLAEVEKLLKNEEISIYKKPSKGIKLNISERDKRQ
                     LTAKFIHKFWSRNWYIKQEQESFYQAFEKIQADVNGIFSEDDLSIIIDILRNFSRERK
                     FEFSDYEFQSLVIHLAIAVERIRKGEYIEESLYIGNIENNQMSNTNYLAKQLEEKFSI
                     LLPKSEIGYINLHLVAADQHRDTNELKNTSTNPCETNTNNISNLREIIKNTLYETAYD
                     KDLIDGLTSHVHSSINRLKYGLSIKNPYVNTIKQNFSLSFEQALDLKKVIEFIYDIRI
                     NDDECAYIALHFEAFRERMKELPQNIRVILVCSTGLGSSRLLSARIKKYFPMIKIEKV
                     LSVQELVSSKIDADLVISTIYLELETIPTIVVSPILNESDIRTLERNIQNVSCFTSKK
                     YKSFRNLIFEENILLNIEASNYEDAIKHITDNLIKKGFAKEGIAKSAIERENLSYTSF
                     QSIATPHANPSYIKKSNISVATLKNPITWGNEKVEVIFFISLENDKNLELDEIYEVFY
                     DFIDNKKNINDILSATSTKEVYDLLVEESI"
     gene            4844..5305
                     /locus_tag="KRL67_002438"
     CDS             4844..5305
                     /locus_tag="KRL67_002438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBH1523310.1"
                     /translation="MKISDVLKKEQIVFNLDTNTKEETIKKLSSIFVENGIVDNEEEY
                     VKSVLEREEHSTTGIGNGIAIPHGKSDAVKKSSIIFAKTKHKIEWDALDEKPVDSIFL
                     LAISNIDKDSNHLVLLSKLATKLMDDDNVDALNLANSEQEIINIFSEEGEM"
     gene            5308..5628
                     /locus_tag="KRL67_002439"
     CDS             5308..5628
                     /locus_tag="KRL67_002439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_463956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HBH1523311.1"
                     /translation="MKRKIIAVTACATGVAHTYMAAQALKKASKKMGHLIKVETQGAT
                     GIDNELTTKDAQIAEVVIFAVDTKVRNAERFEGKEILEVPVNAPIKDAEKVINDALKL
                     IDTK"
     gene            5644..6744
                     /locus_tag="KRL67_002440"
     CDS             5644..6744
                     /locus_tag="KRL67_002440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HBH1523312.1"
                     /translation="MLKKIVSELKKHVLTGISYMIPLVIAGAMIMAISRVGGSFYNIP
                     DIWDAKYAESASSIVRLLHDLDGFGGTALGLMFPVIAAFIGFSIADKLAIVPGLVGGM
                     IAKDIGAGFLGALAVGLIAGYTCLFIKNHVKLPKSAASIVPIFIVPVFGTLITVVLIN
                     YVIGIPFATLNTGLENWLNGLSGTNQILMAAIIGAMMAVDLGGPVNKAALTTSLALLT
                     SGIYAPNTAAMVGIVIPPLGLGLATILAKQKYNKQLREAGKSSLIMGLIGVSEGAIPF
                     AVESPLKVIPSCVIGTAIASAMAVGLGSVNATPISGFYGWFTVENWPMYVLSIAVGTV
                     IVAGLVIVLRGNPVIEDDEFEDDDFDEAEWES"
     gene            6864..9464
                     /locus_tag="KRL67_002441"
     CDS             6864..9464
                     /locus_tag="KRL67_002441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HBH1523313.1"
                     /translation="MIKAHIVNHTHWDREWYFTSGDALVLSEQLFTDVIDELERNPDV
                     SFVLDGQLSILDDYVQLHKEKIEIIKKLIKEDRLHIGPWFTQTDAFFARGESILRNLM
                     IGIFESKKYGKYMKIGYLPDTFGFNAQMPILLKHVGLDNIFFWRGIHLGKQVQSPYFK
                     WKSLNGESHIYAVNMPHGYGTGMLLEPSLKYVNGRLDPAIDFIKSYTDVDEVLIPSGN
                     DQLNIIGDFKNKIKAINDIGKYSYITSSYQEFLKYIKSIESLESYRGEFREPVLARIH
                     KSIGSSRMDIKLACDKLENKLIKRIEPLLVIAKKSKIEISNQLLINTWKKLLEGQAHD
                     SLAGCVTDTVTDDILHRIREANEICDSIENTIVKKISEGLKLSKNNILVFNTEAKRFN
                     GYKEIQVVSDSKNIYFKDNLDATIIEETYVKPRKNVLEETPAGNVFIEEPGYYILKVR
                     INVQLPALGYKVVSFELSDKEMVSLDKSNDTFISNDNYKIIYEDGRLNLQLKDGTYIS
                     NFLTLKDSGNAGDTYDFSPLKNDEDIKLSFDRVYTEKALGYEKLVVKGTTLLPLTLED
                     RKNKYLNGKLNIKVSIVLSKENPLMDVKLCVDNTIYNHRLRVHIKTDIKDNKNIASLP
                     FGYITRENGVLDNWEDIYSEMPIDLEPLESNITLTNQNRSCTVFTRGIKEYQHIEDEI
                     VLTLLATTDELGKPDLLYRPGRASGDTTKKGHIRIKTDKAQILKKLEFSFAIYMDSKS
                     FDELEIANLTHNYLRECVNYQLQDYNFFLYRIDNKIQKSIVNKTVSRELEVISLPENY
                     LISACYPSYYSKDKFIIRLENPSSNRMILDEQIFKNRNGKIVNAIEDVEEEQVYEIAP
                     FDVISILLDL"
     gene            9796..10989
                     /locus_tag="KRL67_002442"
                     /pseudo
     CDS             9796..10989
                     /locus_tag="KRL67_002442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012032602.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
     gene            complement(11191..11634)
                     /locus_tag="KRL67_002443"
     CDS             complement(11191..11634)
                     /locus_tag="KRL67_002443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009250215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HBH1523314.1"
                     /translation="MFRYVHTNIIAKDHQKLIDFYKDVFHCKSIGETRDLRGEWLDNL
                     TGIPNAHILGEHLCLPGYEKDHPTLEIFSYDTMENSCNAINKCGIAHLAFEVDDVEET
                     LQLLLQKGGSQVGELVKTKYEDGKKAVFVYATDCEGNIIELQSWS"
     gene            complement(12218..13844)
                     /locus_tag="KRL67_002444"
                     /pseudo
     CDS             complement(12218..13844)
                     /locus_tag="KRL67_002444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003444896.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1182 family transposase"
BASE COUNT         5388 a         1683 c         2202 g         4829 t
ORIGIN      
        1 ttaaacttaa tagatatttt ctatataaga aagatggtca taatatgtca atttgaccat
       61 atttttgata taattaaaac acaaataatt tagtaacaaa ggaggcagaa cttgaattta
      121 ttaaaaaaat atcctatacc tattgcaagt ttaattttat caatctttac tttaggtaac
      181 ttattacaat cttatagtga atcattacgt aatgtaattg gatttatagg atttattttg
      241 tatgcaattt atatagtcaa aataatatta cttagaaagg gtgttaaaga gcaattagaa
      301 aatcctttaa tatcaagtac atttccaact ataacaatgg caacaatgat atttgctaca
      361 tatgtaaaac cattatcttt agaattaagt attatgattt ggagcatagg aattgtgggt
      421 catcttgtgt taatagtctt atttagtaag aagttcttag ttaaattctc aataaaaacg
      481 gtatttccat cttggtatat agtatatgtt gggatagttg cagctagtgt tacagcccca
      541 gcatttaatc aacttttgat tggcaaaatt gctttttgga taggatttgt atcttatata
      601 gttcttatac cagtagtttt aaagagagta tggataatca aggaaatgcc tgaaccatct
      661 ttaccgagta tagctatatt agctgcacca ggttcactat tattggctgg atatattaat
      721 tctttttcag ataaaaataa tgtagtgtta tacttgttat tcatattatc aattgtattt
      781 tatattatag ttttgggata cttacccaaa ttattaagat tgccttttta tccgagtttt
      841 gctgcattta cattccctat ggcaataagt gcaatgggta tgaaactaat gaatggttat
      901 ttaactaaat caggtcattt tataagttgg atttcatatg cttctaagtt ggaagagata
      961 attgctacag tgattatttt atatgtttta gttttattta ttatatatct tagtaaatct
     1021 gaaactacat ctaaagctaa ataaaaatta ttaaatatgt gtatttaata ggaatattga
     1081 tataaatgta tgaagataag ataaaccata gaaacttaat ttagcttaat tttattggag
     1141 gaaaacaaat gaaaacaaaa gaagaatatt taaaagaagt tagaaacata tcagaagaat
     1201 actttagaaa aggagagttt ttttgctctg aagcagttct tcagacaata aatgatgctt
     1261 taggtcaacc tttaagtcca gagataacta aattagcatc aggatttccg ataggacttg
     1321 gaaaagctca atgcctttgt ggagcgatat ctggaggaga gatggcttta ggtatagtat
     1381 atggaagagt acatggagaa actatgaatc caaaaatgtt tgaatatgct aaaggtttgc
     1441 atgattatat taaaaaagag tatggtgcaa catgttgtag agctattaca aagaagtggg
     1501 atggtgataa ctttatgtct cccgaaagga aagagcactg tattaaaata acaggacaag
     1561 ttacagagtg ggttgcagat aagcttatag aagatgaaaa acttaatatc aaatagtgca
     1621 atattggttg gtgaatttat aaagttaaat ttacttagag tataagaggc ttggtattct
     1681 cgtattgata gaaaaattta tagcatatgt atttaatgaa atatactttc atttagagtg
     1741 tggtttaatt tcataaatac atatgctatt attttaagta tataagcaaa tttatttaaa
     1801 taaatagaaa tagttaattt atatgtacaa aaatatatat gattagtgta aataaatacc
     1861 tattttataa tactcaacat aaagacacca gacatgatta gtattatacc aataagaata
     1921 gcaagccatt cttttttgtt cttcttttcc ttaaaaaata taattcctcc caaagtggct
     1981 actatcacac aactctgaga gatagaaaaa cttattgtaa ctccaaatgt attagttgtt
     2041 atgaacatac ctaaatttgc tattgaccac aatattccag taattaaatt gaataaaaca
     2101 ttttccctat atacaatctc tttagaaaaa tacttgacaa taatagatga agttatcaac
     2161 ataccaacag attgaggtaa tataattcta tatccttcaa ttaaaaatat ttgattagtt
     2221 acaacatata atgataaaaa tagagatgat aataatacaa ctccaacaca ttttaacata
     2281 tttttatctg tagatttttt agtttgataa tttgtgagaa gtataccaag tattatacat
     2341 aatattgata aagaaccaat cagtaacatt ttaaaactgg tccattcatt aaacaagatt
     2401 acagctagta atgtcgtaaa agttaattgt gaaccatttg aaatcggcat tactttagat
     2461 acactagcaa tttgaattgc tttaaactgc attaattgtc ctaaactcca gaatatgcca
     2521 gaaaccatac ttatacaaaa tgaaaaaaat gtaatttcag gatgtataac cagagttatt
     2581 atagtggaaa atataaacgc acttatggat gtacctaaaa gctgttcatg aggagaactt
     2641 ttctttagat ttgcaactat gggcatcaat ccccatccca ttacaggaag taaagcaata
     2701 attattgtta gcattaaact atttcctttc atattaaatt tagacagctt ttaatttatc
     2761 atctaaaatt aaagtgttca atacataaaa attaccgtat ttaatcagga aatattagta
     2821 gttttttaga aaaacatttt ccattaaaat aaagacaaag ggaaggagta aaattgaaaa
     2881 tgttaactga tatgattcaa aatcgtgaaa ttaaaataat aaagttttta ttaaaagaag
     2941 gtcatacaac tgtaaaagca atagcagatg atttagatgt ttcagaaaaa acagtttcaa
     3001 aatctcttaa agaagtagaa gcagtgctta aaaagatagg tctttcttta ataagaaaac
     3061 ctaagatagg tgtttatatt gatggagatt taaaaaaaat acttccttac ctagataata
     3121 aaggaagtca aattcctaca accagagagg agagagttat ctatatatat tcagtacttt
     3181 taaagcgtaa tgactatatt actatacaac aactttctga cgaattatat ataagtcgtg
     3241 gaactattga aagggattta gcagaggttg aaaaactgct aaaaaacgaa gagatttcca
     3301 tatataagaa acctagtaag ggaataaagt taaacataag tgaaagggat aaaagacaac
     3361 ttactgctaa gtttatacat aaattttgga gtagaaactg gtatattaag caagagcaag
     3421 aaagctttta tcaagcattt gaaaagatac aggcagatgt aaatggtatt ttttcggaag
     3481 atgacttatc aataattatt gatattctta ggaatttcag cagagaaaga aaatttgagt
     3541 tctctgatta tgaatttcag tcattagtta ttcatttggc aatagctgta gagagaatta
     3601 gaaaaggcga atatatagaa gaaagtttgt atattggtaa tatagaaaat aatcaaatgt
     3661 caaataccaa ttatttagca aaacaattag aagaaaaatt ttcaatatta ttaccaaaat
     3721 ctgaaattgg atatataaat ttacatttag tagcggctga ccaacataga gatactaatg
     3781 aactaaaaaa tacttcaact aatccttgtg aaacaaatac aaataatata tcaaatcttc
     3841 gggaaattat aaaaaataca ttgtatgaga cagcttatga caaggattta attgatggat
     3901 tgacttcaca tgtacattca tcaataaata gactgaaata tggacttagt attaaaaacc
     3961 catatgtaaa tacaatcaaa caaaactttt ctttatcatt tgagcaagct ttagatttaa
     4021 agaaagtaat tgaatttata tatgacatta ggataaatga tgatgaatgt gcttatatag
     4081 cattacattt tgaagctttt agagaaagaa tgaaagaact tccacaaaat attagagtaa
     4141 ttctagtatg tagtacaggg ttaggttcat ctaggttatt atctgcaaga ataaaaaaat
     4201 actttccaat gataaagatt gaaaaagtac tttccgtaca agaattagta tccagtaaga
     4261 ttgatgcaga tttagtaata agtactattt atttagagtt agaaactatt ccaaccatag
     4321 tagttagccc tattttaaat gaaagtgata taagaacctt ggaaagaaat atacaaaatg
     4381 tttcatgttt tacttctaag aagtataaat ctttcagaaa cttaatcttt gaagagaata
     4441 tattgttgaa tattgaagct tcaaactatg aagatgcaat taagcatata acagataacc
     4501 taataaaaaa aggttttgca aaagaaggta tagctaaaag tgcaattgag agagaaaatt
     4561 tatcttacac atctttccaa tcaatagcta cacctcatgc taatcctagt tatattaaaa
     4621 aatctaatat atctgttgca acgttaaaaa atcctattac atggggaaat gaaaaggtgg
     4681 aagttatttt ctttatttca ttagaaaatg acaaaaacct agagcttgat gaaatatatg
     4741 aagtttttta tgacttcatt gacaataaaa aaaatataaa tgacatatta agtgcaactt
     4801 ccacaaagga agtgtacgat ttgctagtgg aggagagtat atagtgaaaa tatcagatgt
     4861 attgaaaaaa gagcaaattg tttttaatct tgatacaaat acaaaggaag aaacaataaa
     4921 aaaattaagt agtatatttg ttgaaaatgg gattgtggac aatgaagaag aatatgttaa
     4981 gtctgttttg gaaagagaag aacattcaac tacaggtatt ggtaatggta ttgctattcc
     5041 tcatggaaaa agtgatgctg tcaaaaaatc ttctataata tttgcaaaga ctaagcataa
     5101 gattgagtgg gatgctttag atgaaaaacc agtagatagt attttcttat tggctataag
     5161 caatattgat aaggatagca atcatcttgt actattatct aaattagcaa ctaaacttat
     5221 ggatgatgac aatgtagatg cactaaattt agccaattca gaacaagaaa taatcaatat
     5281 atttagtgaa gaaggagaga tgtagagatg aaaagaaaaa ttatagcagt tacagcatgt
     5341 gcaacaggag ttgcacatac atatatggca gctcaagcat taaagaaagc ttcaaaaaag
     5401 atgggtcacc ttataaaagt tgaaacacaa ggagctacag gaatcgataa tgagttaact
     5461 acaaaggatg cacaaattgc agaagtagta atatttgcag tagatactaa agtcagaaat
     5521 gctgaacgtt ttgaggggaa agaaatacta gaagtaccgg ttaatgcacc tataaaggat
     5581 gcagagaaag taataaatga tgcactaaag ttaattgaca ctaaataaat ggagggaaat
     5641 gtaatgttga aaaagatagt ctctgagtta aaaaagcatg tcttaactgg aatctcatat
     5701 atgattccat tagtaattgc aggtgctatg ataatggcaa tatctcgtgt aggtggttct
     5761 ttctataata taccagatat atgggatgct aaatacgcag aaagtgctag tagtattgtt
     5821 agattattac atgatttaga tggatttgga ggtactgcat taggattaat gtttcctgta
     5881 attgctgcat ttataggatt ttctatagct gataaattag ctattgtacc aggtcttgtt
     5941 ggaggtatga ttgctaaaga tataggtgca ggatttttag gagctttggc agttggtctt
     6001 atagcaggat acacatgttt atttattaaa aatcatgtta aattaccaaa atcagcagct
     6061 tcaatagtac caatatttat agtaccagta tttggaacat taatcactgt tgtattaatc
     6121 aattatgtga taggtatacc ttttgccaca ttaaatactg gattagaaaa ttggttaaat
     6181 ggattatctg gtacaaatca gatattaatg gcagctatta taggggctat gatggcagtt
     6241 gacctaggtg gaccagttaa taaagcagct ttgacaacat cacttgcatt attaacaagt
     6301 ggaatttatg cacctaatac tgctgcaatg gtaggaatag tgataccacc actaggattg
     6361 ggacttgcta ctatactagc aaaacaaaaa tacaataaac agttacgtga agctgggaag
     6421 tcatctctta taatgggttt aattggagta agtgaaggag caattccttt tgcagtagaa
     6481 tctccattaa aagtaatacc ttcatgtgta attggaacag caattgcttc agctatggca
     6541 gttggtttag gttcagtaaa tgcaactcca attagtggat tttatggatg gtttacagta
     6601 gagaattggc caatgtatgt gttatcaata gcagttggaa cagtaattgt agctgggtta
     6661 gttatagtat tgcgtggaaa tccagtgatt gaggatgatg aatttgaaga tgatgatttt
     6721 gatgaagcag agtgggaatc ttaaaattta ataactattt aagacgtgtt ataaatcata
     6781 ttcaatatga atcttatact aaataaagtg ttaaatattc agtttagact taaatataaa
     6841 agataaagaa cagaggagaa taaatgataa aagcacatat tgttaatcat acacactggg
     6901 atagagaatg gtattttact tctggagatg cattagtact tagtgaacaa ctatttacag
     6961 atgtaattga tgaattagaa cgtaatccag atgtaagttt tgtacttgat ggacaacttt
     7021 ctattttgga tgattatgta caattacaca aagaaaaaat agaaatcatt aaaaagttaa
     7081 taaaagaaga tagactacat attggaccat ggtttactca aactgatgca ttctttgcta
     7141 gaggagagtc tattttaaga aacttaatga ttggaatttt tgaaagtaaa aagtatggta
     7201 agtatatgaa aattggatat ttgccagata catttggatt taatgcacag atgcctatac
     7261 tattaaagca tgtaggtctt gataatatat tcttttggag aggtatacat cttggtaaac
     7321 aggttcaatc accatatttt aagtggaaaa gcttaaatgg agaaagtcat atatatgcag
     7381 taaatatgcc acatggatat ggaactggta tgcttttaga gccttcatta aagtacgtta
     7441 atggacgctt agacccagct attgatttta ttaaatcata tactgatgta gatgaggtgt
     7501 taataccttc tgggaatgac cagttaaata taataggtga tttcaaaaat aaaattaaag
     7561 cgattaatga tatcggaaag tattcatata taacgagttc atatcaagag tttttaaaat
     7621 atataaaatc catagaatcg ctagaaagct atagaggtga atttagagag cctgtattag
     7681 caagaataca taaatcaatt ggttctagta gaatggatat aaaattagca tgtgataaac
     7741 tagaaaacaa gctgattaaa agaatagagc cattattagt aattgccaaa aagagtaaaa
     7801 ttgaaataag caatcagtta cttataaata catggaaaaa acttttggaa ggacaagcac
     7861 acgatagttt agctggatgt gtaactgata ctgtaacaga cgatattctt caccgtataa
     7921 gagaagcaaa tgaaatatgt gacagtattg aaaatactat tgtcaaaaag atttcagaag
     7981 ggcttaaatt atctaaaaat aatattttag tatttaatac agaagctaag cgttttaatg
     8041 gatataaaga gatacaagtt gtaagtgata gtaaaaatat ttattttaaa gataatttag
     8101 atgctactat aattgaagaa acttatgtaa aaccgagaaa aaatgtatta gaagaaacac
     8161 ctgctggcaa cgtatttatt gaagaaccag gatattatat attaaaagta agaataaatg
     8221 tacagcttcc tgcacttggt tataaagttg ttagttttga attaagtgat aaagaaatgg
     8281 tatcacttga taaatctaat gacactttta tatctaatga taattataag ataatctatg
     8341 aagatggaag attaaattta caacttaaag atggaactta catatcaaac tttttaacat
     8401 taaaagatag tggtaatgca ggcgatacct atgacttttc tccattaaaa aatgacgagg
     8461 atataaaact ttcatttgat agagtgtata cagaaaaagc tttaggatat gaaaagttgg
     8521 tggttaaagg tactacctta ctaccactta cattagaaga tagaaaaaac aaatatctta
     8581 atgggaaatt aaatataaaa gtaagtattg tcttatccaa agaaaatcca ttgatggatg
     8641 taaaactttg tgttgataac acgatatata atcatagact acgtgttcat ataaaaactg
     8701 atataaaaga taataaaaac atagcatcat tgccatttgg atatataaca agagaaaatg
     8761 gagttttaga caactgggaa gatatttata gtgaaatgcc tatagattta gaacctttgg
     8821 aaagtaatat tactctaaca aaccaaaata gaagttgtac tgtatttact agaggaataa
     8881 aagagtatca acatattgaa gatgaaattg tattgacctt acttgctact acagatgaac
     8941 taggaaagcc tgatttatta tatagaccag gacgtgcttc tggtgataca actaaaaaag
     9001 gtcatataag aattaaaaca gataaagctc aaatacttaa gaaattagaa ttttcatttg
     9061 caatatatat ggattctaaa tcatttgatg aattggaaat tgcgaattta acccacaatt
     9121 atctaaggga atgtgtaaat tatcaattac aggattacaa tttcttttta tatcgtatag
     9181 acaataagat tcaaaaatca attgtcaata aaactgtaag tagagaatta gaagtaatat
     9241 ctttaccaga aaactatctt atatcagcat gttatccttc ttattatagt aaagataaat
     9301 ttataataag attggaaaat ccaagtagta atagaatgat attggatgag caaatattta
     9361 aaaatagaaa tgggaaaatt gtaaatgcaa tagaagatgt agaagaagaa caggtatatg
     9421 aaatagcacc ttttgacgtt atttcaatat tattggattt ataagtgtaa aagtaagttt
     9481 attgtttaaa ggggctgtcg cattagcaaa ttaaatttgt tctgtgacag ctttttttac
     9541 ttatcttaat ggggacattt ttaattgttt tatatagagt ttatgttcta aataaaatta
     9601 tgcataacaa ataaaggcat aaaaattttt ttcagataat atttagtaaa tttagaatac
     9661 ttttttttaa tgatgaagat attccgggtc tttaaaataa agtgtgtatt cttctaaaaa
     9721 atggtgataa ttaaaatatt aaatatcact atataaagga aggtgcatac tttttatgtt
     9781 taacaaaaac gaaatattaa aaaacttatt gaagaatacg atgttaaaac aactacagac
     9841 attcaaaata tgttaaaaga tttatttgca agtacaattc atcaaatgtt agaagcggag
     9901 cttgatgacc atttaggtta tgatagatat gataataaga ataaaaatac taaaaattct
     9961 agaaatggat atagaaataa aaatgttaaa tcagattttg gagaaataac tttaaatatt
    10021 ccaagagacc gaaatggaga ttttgaacca aaggtaatta gaaattatga aaatgatata
    10081 tcgggagtag agaaccaagt gattggaatg tactcaaaag gcatgtctac tcgagatata
    10141 tcaagccacc ttaagagtat ttatagaatt gatatatctc attctctaat agctaaaatt
    10201 actgatagag ttctaccttt agctcatgaa tggcaaaaca gacctcttga tatggtttat
    10261 ccaattgtat ttatggatgc tatacattat aaagttagaa gtgaaggtag aataataaac
    10321 aaagctgcat atatagccat aggtgttaac ttagaaggta taaaggaggt cttaggaata
    10381 tggattggag aaaatgaaag ttcaaagtat tggctaaagg tacttacaga tgtcaaaaac
    10441 agaggagtta aggatatatt aatagcttct gtagatggat tagttggatt ttctgatgca
    10501 attaaagcag tatttcctga tactgaaata caaatatgtg ttgtacatca aattagaaat
    10561 actttaaatt atgtatcata taaaaataga aaagaatttg caaaagattt aaagaaagta
    10621 tatgctgcaa ttacagagga agtagcatta agtgagctat cgctcttaga ggaaaaatgg
    10681 ggtaatcaat atgcgatagc attaaaaaat tggcgaagta attaaaatga gctagcaaca
    10741 taccttaaat atcctcaaga gataagaaca ttgatatata ctacaaattc aatggaaagt
    10801 tacaatatct tattaagaaa agttactaaa tcgaagtcta tttttccttc agatgattct
    10861 ttgcataaat cattatgctt agcaactatg gatatttcta aaagatggac tcaaaagatt
    10921 agaaattgga ctcaaatttt agctcattta agtatttatt ttgaagaaag aattttaact
    10981 ctaaattaat aaagaaatat ttcaaaaaaa ttaagaaatt ttcttaaagg ggtaataatt
    11041 tttgcaaaaa tggaaaaaat atcaaaataa ggctatgctt tttaaagcta tagccttacc
    11101 aataattgtt taattatcac ttaaaaaata aacttttatt taaggaatat acataaatat
    11161 ttccagactc aatatgttca cactttatac ctatgaccag ctttgtaatt caataatatt
    11221 cccctcacaa tctgttgcat agacaaaaac ggctttcttt ccatcttcat attttgtctt
    11281 taccagttct ccaacttgac tgccgccttt ttgaagtaag agttgtaaag tctcttctac
    11341 atcatccact tcaaatgcaa gatgtgctat tccacattta tttatggcat tacaagagtt
    11401 ttccattgta tcataagaaa aaatttcaag cgtaggatgg tctttctcat atcctggtaa
    11461 acaaagatgt tctcccaata tgtgtgcatt aggaattcca gttagattat caagccattc
    11521 tccacgcaag tcccttgttt ctcctatgct tttacaatga aatacatctt tataaaaatc
    11581 tatcagtttt tgatggtctt tagcaataat atttgtatgt acatatctaa acatatattt
    11641 tcccctttca aattctaatt tgtaagaata ttatacaact attcaatacc attagcaact
    11701 ctaaataact cttatatgta caatataaat ataatcatta ctttttatat tcaaaaaaga
    11761 caatgaaatc tgtgaacctt tcttattatc tctaatataa aaatatcttt attcataaat
    11821 ttataaatat tattattttg atatgtattt acatataaaa agacaataga atctgcgaac
    11881 ctttcttgtg tggactttta tttatatttt atgtaatata tgcttatatt ttattatgta
    11941 tgtaaaataa aacatgaaag gaggtaaaaa atgactaata aactattatt tgaagattta
    12001 aaaccaaatg ctagaatatc caaatcaaaa ataaaaaaag taatggggat gtcgcattag
    12061 caaattaaat ttgttctgtg atagcttttt tacttatctt aatgggtata tttttaattg
    12121 ttttatatag agcttatgtt ctaaataaaa ttatgcataa caaataaagg tataaaaata
    12181 ccatttttca gataatattt aactataaat aataaattta gaatacttct tttaaatgat
    12241 gaagatatat tccacgttta ttttttatta ttttattgtg tagtctagaa atgttatgag
    12301 ctatcgctac aataatacat tctgatagaa cattttcttt acctcggctt aaaaatcttc
    12361 taaatttcat atttttttta cttgagcgaa agctccttct gcttgtatac ttctatttat
    12421 tctaagttga caaccttccg ggcttataat tctctttaaa ttttcttttc ttttttcatg
    12481 aaataactta gatacttgta aatttttagt tctattttca agaggaatct tactattatt
    12541 tcccttaata caactgcttt tatattaaca atcacggcat tctttacaag tataacaagt
    12601 tattttactt ttatatcctg tttttgattt tctattgatt attttattag caaatagttt
    12661 tttgccattt ttacaaatat aataatcatt tttttcatca taatccatat tattaatacg
    12721 cccaatctca tttttatatt ttctagtttt actaatttca taatttgcaa gctttataaa
    12781 agataattga ttattacact ctaaaaatgt atagttttcc tcactttcat acccagaatc
    12841 agctactatt ttaggatatt taaattttgt atggtcttca atagatttta aaaatggtat
    12901 taatgttgta gtatcagtag gttaatgctc tgaacttacc caaactatgt attctgaatc
    12961 tacaccaaat tgtatattat aagctggttt caattgccta tttttcatat ggtctttctt
    13021 cattctcatg aaagttacat cattatctgt tttagaataa ctatttcgtt cacccattat
    13081 atgaaggtct ttagtatatt tttcaatcta tcgatatagt tatttaattt ctctatagat
    13141 ttttgaacaa tagatttttt ctttcctttt tcatgaacaa atgttatatt tgattctttt
    13201 gatatttttt taagtttctt taaaagtttt tttaaatgat atattttgat tttatttcta
    13261 taaattattt taataccaaa atcaatctca cattttaata tgaaattaga catatcattc
    13321 attattctaa ccatattttt gtttatagat tttttccaaa caaaagtata tctattagca
    13381 gatgactcta tttttgtacc atcaataaat agaacatctt ttgaaatccc cttattatct
    13441 ccaagaaagt ttgtaaactg tgcaagcaaa tttttgctta cttgtgaaaa atgaatactg
    13501 cgaaatcttg caattgttga atggtctgga gcggaggctc cgcctaaaag atacataaag
    13561 ttaatatctc ttttacatgc ttgttctatt tttcttgaag aataaataca gttcatattt
    13621 gcatatatta taattttcaa aagttgtcta ggggttactc tatttcctct aattcgagaa
    13681 taagtcttat ataacttttc taaattcatc tcctcaataa tttgacttaa tagccttaca
    13741 gaatcatctt ttggaatcat ataatcaata tttagtggta atttaaattg ataattatca
    13801 ccatgagtag tataatcaat ttgtatagta tcttgttttg acatacaatt attatacaaa
    13861 aatcccaggc tcttttgaac ttgggattta tttttttata taaaaaaggg cttagcacta
    13921 ttttttagtg cgacagcccc tttttaattt gtataaattt gggtaaatat taatgaatgg
    13981 ttttagtatt taaatatgaa tttagtattt aatgtagaaa atatagattt agtatttaat
    14041 atagaattta ttgaagtaga ataattagtt taacaggata agcagtttaa acaaagaatt
    14101 at
//
LOCUS       DAEPXF010000096        13844 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain HB27
            SAMN13376389-rid12479423.denovo.101, whole genome shotgun sequence.
ACCESSION   DAEPXF010000096 DAEPXF010000000
VERSION     DAEPXF010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13376389
            Sequence Read Archive: SRR10518432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 13844)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13844)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001069624.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001069624.1
            Genome Coverage       :: 315x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/15/2021 01:54:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,165
            CDSs (total)                      :: 4,076
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 89
            rRNAs                             :: 10, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 10 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 19 of 69
            Pseudo Genes (incomplete)         :: 44 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 10 of 69
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13844
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13376389-rid12479423.denovo.101"
                     /strain="HB27"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="China: Hebei"
                     /collection_date="2017"
     gene            255..1148
                     /locus_tag="KRL67_002445"
     CDS             255..1148
                     /locus_tag="KRL67_002445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfide/dihydroorotate dehydrogenase-like
                     FAD/NAD-binding protein"
                     /protein_id="HBH1523315.1"
                     /translation="MSNKIVSKRQLTDSIYLMEIEAPRVAKSSQPGQFIIIKNDEKGE
                     RIPLTIADYDREKGTVTIVFQTVGASTKKLAMFEENDFVMDFVGPLGQASEFIHEDIE
                     ELKNKKILFVAGGVGSAPVYPQVKWFKEHGLDVDVIIGARTKELIILEDDMKKVAKNV
                     YVSTDDGTYGFNGRVTDLLKDLVDNQGKKYDQAIVIGPMIMMKFMCQLTKELNIPTIV
                     SLNTIMIDGTGMCGGCRVSVGNETKFACVDGPEFDGHLVDFDQAMRRQSMYKTQEGRA
                     MLKLEEGDSHHHSNCGCGGNK"
     gene            1148..2542
                     /gene="gltA"
                     /locus_tag="KRL67_002446"
     CDS             1148..2542
                     /gene="gltA"
                     /locus_tag="KRL67_002446"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent glutamate synthase"
                     /protein_id="HBH1523316.1"
                     /translation="MDAKKVKVPVREQEPAVRATNFDEVCLGYNKEEAMAEANRCLAC
                     KKPKCVGGCPVGIDIPGFITKIKEDDIEGAAKVIAKSSSLPAVCGRVCPQESQCEGVC
                     ILGIKSDAVSIGKLERFVADWSKENDINLSDTEPKKNQKVAVIGSGPAGLACAGDLAK
                     KGYDVTIFEAMHEPGGVLTYGIPEFRLPKQAVVQPEIDNIRKLGVKIETNVIVGKTIT
                     VDELIEDEGFEAIFIGSGAGLPMFMNIPGENANGVFSANEFLTRVNLMKAYRDDYDTP
                     ISSGKKVAVVGGGNVAMDAARTALRLGSESYIVYRRSEKELPARAEEVHHAKEEGIIF
                     NTLTNPKEILVDENGYVKGMVCIRMELGEPDDSGRRRPIEIEGSEFVLDVDTVIMSLG
                     TSPNPLISSTTKSLDINKKRCLITDENGQTSKEGVFAGGDAVTGAATVISAMGAGKTA
                     AASIDEYLKAKVNA"
     gene            2908..5127
                     /locus_tag="KRL67_002447"
     CDS             2908..5127
                     /locus_tag="KRL67_002447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HBH1523317.1"
                     /translation="MKKREKVFDGLVFKHILILLILLVLIDLTSTIIYASNSMEITSR
                     NMIEASKRELENYLEVNISLLKALSQDDRFSNGETSLIEKGKLLRPYQKEYNLFMIGI
                     TDTKGNVSSTYRENVNSIKDKPSFEKAIKTKQVVVSDIEVSNVTGDKVFIIYVPIIKN
                     NEMIGTIFASFYFQDVNNLISRSNFDDSIKFLMIDKDYTIISHPNKKYVNDKSKILDL
                     EGNIIGTTKSEILKNINKKCQGNFLSWDNWRLYNVKYTNIKWTNWTLVSKCNIFKNFK
                     NLIVNFMIKLYFYIVIFMILWKLSNAKLIEQLKKLAYYDSLSGIKNKEKFRKDSMYIL
                     KNYYQDNFYLVQLDVNKFKYINEMFGYSEGNKILIHISQVLNNNTNKYEICARMDNDH
                     FILLIACNTEDELLNRLSKINKEICNLITTNSSKYKIVMSSGIYKITKKDDIKKIDLL
                     IDRANIAAKSKKEKYEHSYSFFNEDTRNRLYKEKRLEDNMNKALEKGEFIVYYQPKYS
                     LGDVNEIEGAEALIRWNSPEFGFISPIDFIPLFEKNGFIVNIDMFVFEEVCKTLNKWI
                     NKGYTPVPISVNMSRVHLYRDNFIENITDLISKYNISPEFIELELTESVVFDNLNILI
                     DIMKKIKEIGFLISMDDFGSGYSSLNLLKDLSFDILKLDRGFLIETTDTKRGKIIISK
                     IVEMAKAIDIKVICEGVETYEQVEFLKEIGCDKVQGYLFAKPMVLDEFEKHLNFKFD"
     gene            5274..6020
                     /locus_tag="KRL67_002448"
     CDS             5274..6020
                     /locus_tag="KRL67_002448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014466367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBH1523318.1"
                     /translation="MNILSIRNISKTYCGNIPFKALDKVSLNIEKGEFVSVMGPSGSG
                     KSTLLNIISTVDRQSEGEVILDGYDVSKLEGEKLAEFRRKQLGFVFQDFNLIDTLTVG
                     ENIMLPLTLDGASIKDMNIQTKSISKFLGIDKILDRKTYEISGGQAQRCAIARAIINK
                     PAILLADEPTGNLDSRSTDDVLKLFTRINKEQNVTTLMVTHEAYSASYSDRVIFIKDG
                     CIYTEIKKSESSNSFYSDILAVLSQIGGVR"
     gene            6020..7900
                     /locus_tag="KRL67_002449"
     CDS             6020..7900
                     /locus_tag="KRL67_002449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBH1523319.1"
                     /translation="MDFRKFAYNNVVRNFRAYLAYFLSSVFSIMIFFVFAVSMFHPII
                     TKSGIQSGSTAFMALMTSELIILGFSLLFILYSLGNFLKYRLKDFGVLMIIGMSNRQL
                     KKLILIENLIIGFLAVMLGILLGLSISKLFLLYLSKLFYMNLTEFYFPVKAILLTIVS
                     FMILFLITGPISLKLLSKKSILELLIGTKKPKKEIKSSKLFGIIGAVCLILGYAMIIF
                     GNKLKINGGMYTTALLITLGIFLFFSQFTILILKYLKNNEKFYKNKVNMLLISNLIYK
                     LKDNTRLLFLITILLSATLVAFTTTSTLVSSQGESSKNNFPMVYSYFSAHNNEKEYEE
                     LQQIRDAIKGYDYKELSFPVLKYNREVLLSVKNYNKLSKELGLDKISSKLEKYEGIII
                     PSSISQEHINSLKQLKNYNIKNLNIEVKKSTNKIIFPTGLFSKVVVISDDLYNEVKGE
                     FSKINFHGFDYKNWQSSAKITENLKEKHFNLDREYNRSYFLTLPDMYLVELQQSKILQ
                     FMGVFIGVILFIAVFSFLYFRLYTDEIEDNVKYKKLSKIGLSFKNMNKIVSIEIGTLF
                     FIPYFIAIINSIISLLFLNQVLNNSFNLENLGILLLISSIYSLYFYLLKKNYTRKVWF
                     FD"
     gene            8019..9410
                     /locus_tag="KRL67_002450"
     CDS             8019..9410
                     /locus_tag="KRL67_002450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HBH1523320.1"
                     /translation="MENQQLLGTERISKLLLKYSIPAIIGMLVNSLYNVVDRIFIGNI
                     PGVGPLAITGLGVTMPIMTIILAFGMLIGIGTTTTISIKLGQGKVEEARKLIGNAMTL
                     SVITGIIIMILGILFANKILTLFGASENTLIYAKSYINIILLGTVVNLLSFSLNHSIR
                     ADGSPKISAGIMIVGCLTNIVLDWILIFGFNLGIQGAAIATVTSQALTAILTIGYYIS
                     GKSNLRFSKSNLKLDKKLIKAVFAIGMSPFAMQLAASLVQVISNIALKTHGGDLAIGA
                     MATISSIAMVFLMPIFGINQGAQPIIGFNYGAEKYDRVKKAYLGSLVVATIILCMGMV
                     VIMLFPEAIIGIFNKDPELMNISVNGLRIYLLMLPIVGLSVTGTNFIQSIGKAKMAML
                     LSLLRQVILLIPAVLILPTFLGLQGVWTAQPVSDFIATVITGIVVFRELKRYTPKTEK
                     LNENERLNEITTE"
     gene            9517..10557
                     /locus_tag="KRL67_002451"
     CDS             9517..10557
                     /locus_tag="KRL67_002451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HBH1523321.1"
                     /translation="MEFTKKSKLKNMYVNMNVARMLSKINEYKGRQLLYKKQPKEILE
                     NLEKKSLVDCSESTYIGNQRDSSNFNLEKLISNEVTPRSREELSIVEYRDVVKTINSA
                     YESIPISSQTILELHGYLYKFSSTRGGSYKSDNDFIEHNLKPSEFISIDSSVNKVEKA
                     VEEICEAYNTLIEEDEIDILILISAFVLDFILIHPFKEGNIKMARVLILLLLNKNGYE
                     VGRYISLGKIFDDSSYEYYSNLNYLKASIGSEKADMNAWIEYFLETILTAYEKLDDSL
                     NISDKKRQTKTSRIEKIINSTLGYFTKEDIRDLCPDIPEPTINRVFNNLRKQDKIEVV
                     ARGRSAKWKKKY"
     gene            11043..11696
                     /locus_tag="KRL67_002452"
     CDS             11043..11696
                     /locus_tag="KRL67_002452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HBH1523322.1"
                     /translation="MKKKLLYINVNSKPEDLSSSKTVARKFINKFMEKNKNFEVEEID
                     LYKEHIPRLEYQYFEKRNSIVSEENAKNLDDKDRKELTKIRNLCDQFVSASVYVIAAP
                     MWSLSFPAPLKEYIDCIIQDGKTISFEGNDKPQGILNDIDRSMVYIQSSGGHIPWVLK
                     PVMNKGLNYVESIMKFIGIKKFDELLVDGTGTSEEERQAAIEKASEKIDGIIDDMKF"
     gene            11772..11981
                     /locus_tag="KRL67_002453"
     CDS             11772..11981
                     /locus_tag="KRL67_002453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH1523323.1"
                     /translation="MREEKSNEKYDCYWCNQENNFCVEIKDNIVMIDDGTGTLKQAVF
                     IGYKQIQINLNCSHCQNLNRIKLNL"
     gene            complement(12121..12687)
                     /locus_tag="KRL67_002454"
     CDS             complement(12121..12687)
                     /locus_tag="KRL67_002454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1836 domain-containing protein"
                     /protein_id="HBH1523324.1"
                     /translation="MMNNELNTIILETLNNADITSNDIPSIDLYMDQIISLIDNKLSA
                     NKRFESDKILTKTMINNYSKEGLIKPVKGKKYTKEQILQMIIIYSMKNTLTIQEIKRI
                     LHGVYEKDNFSEKDLVSCYEKFMLIKENQRKNIPDFIESNFENISINPENKDDLLIAL
                     LSLTSMADQLKNISQKLVDRYFPDITKK"
     gene            12848..13501
                     /locus_tag="KRL67_002455"
     CDS             12848..13501
                     /locus_tag="KRL67_002455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HBH1523325.1"
                     /translation="MYQFFLKGRDPISSLTHFIGACLSLLATIILVFQSVSLQETSLL
                     MIVSVSVFGLSLIALYSASSYYHFLKGTPEQELFFRKVDHAMIYVLIAGSYTPICLNF
                     MEKKEGIIFVTAIWIVAFIGIIIKIFWMDAPRWLSTSIYLLMGWAIVFDINAFNSIPK
                     DCLRLLIMEGVSYSIGAIIYIIKKPNISPEFGFHEIFHIFIMIGSLFHFLAVLLYVL"
     gene            complement(13659..>13844)
                     /locus_tag="KRL67_002456"
     CDS             complement(13659..>13844)
                     /locus_tag="KRL67_002456"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH1523326.1"
                     /translation="DDFMYKYDDYVNLTNDLDLPVEIRNKCVRIVSVSENLEDIIIVH
                     KFKLYVVNEKYIKGIME"
BASE COUNT         5422 a         1387 c         2339 g         4696 t
ORIGIN      
        1 gtgtgtatta ggttaaatct ataacaaatt agacatatga tatagactta gtgtttattg
       61 gcatttataa gaaatttgtt aatatcaact atttgtagtt tgggcagtta taattactat
      121 aggggggatg tccaatacaa aacaatcaac caagaactaa tattatatta taaagagtta
      181 tagttttgtg tgtttatgtt tttaattaaa tttaaagtgg gaaaaatata tttgttagaa
      241 tggagaatag cgagatgagt aataaaatag ttagtaagag acaattaaca gatagtattt
      301 atttaatgga aatagaggca cctagagttg caaaatcttc tcaaccaggt caatttataa
      361 taattaaaaa tgatgaaaag ggagagagga ttcctcttac aatagctgac tatgatagag
      421 aaaaaggaac agtaactatt gtattccaaa cagttggtgc ttcaactaaa aagttagcaa
      481 tgtttgaaga aaacgatttt gtaatggact ttgtaggacc attaggacaa gcaagtgaat
      541 ttatacatga agatatagaa gaattaaaaa ataaaaagat attatttgta gctggtggag
      601 ttggttctgc tccagtttat ccacaagtta aatggtttaa agagcatgga ttagatgtag
      661 atgtaataat tggtgcaaga actaaagaat taattatatt agaagatgat atgaaaaaag
      721 tagctaaaaa tgtatatgta tcaacagatg atggtacata tggatttaat ggaagagtta
      781 cagacctatt aaaagattta gttgataatc aaggtaaaaa atacgaccaa gcaatagtaa
      841 ttggaccaat gataatgatg aaatttatgt gccaattaac taaagaacta aatataccta
      901 ctatagtaag tttaaataca ataatgatag atggaacagg tatgtgtggt ggttgtagag
      961 ttagtgttgg aaatgaaact aagtttgcat gtgtagatgg tcctgaattt gatggtcatt
     1021 tagttgattt tgatcaagca atgagaagac agtctatgta caagactcaa gagggaagag
     1081 ctatgctaaa acttgaagaa ggcgatagtc atcatcatag taattgtgga tgtggaggta
     1141 acaaataatg gatgctaaaa aagtaaaagt accagttaga gaacaagaac ctgctgtgag
     1201 agcaacaaat tttgatgaag tttgcttagg gtataataaa gaagaagcta tggcagaagc
     1261 taatagatgt ttagcttgta aaaaaccaaa atgtgtaggt ggatgtccag taggaatcga
     1321 cataccaggg tttataacaa aaattaaaga agatgatata gaaggtgctg ctaaagtaat
     1381 agctaagagc agttcattac cagctgtttg tggtagggta tgcccacaag aaagtcaatg
     1441 tgaaggtgta tgtatacttg gaataaaatc agatgctgta tcaataggta agttagaaag
     1501 atttgtagca gattggtcaa aagaaaatga cataaattta tctgatacag agcctaagaa
     1561 aaatcaaaaa gtagcagtaa taggaagtgg tccagcaggt ctagcttgtg ctggtgattt
     1621 agctaaaaaa ggctatgatg taacaatctt tgaagcaatg catgagccag gtggagtttt
     1681 aacttatgga atacctgagt ttagacttcc aaaacaagct gttgttcaac ctgaaataga
     1741 taatataaga aaattaggtg taaaaataga aactaatgta attgttggta aaacaattac
     1801 agtagatgaa cttatagaag atgaaggatt tgaagctata ttcataggtt caggagctgg
     1861 tcttccaatg tttatgaata taccagggga aaatgcaaat ggagtatttt ctgcaaatga
     1921 gtttttaact agagtaaact taatgaaggc ttatagagat gactatgata cacctattag
     1981 ttctggtaaa aaagttgctg tagtaggtgg tggaaatgtt gctatggacg ctgctagaac
     2041 tgcactaaga ttaggttcag aatcgtatat agtttataga agaagtgaaa aagagcttcc
     2101 agcaagagct gaagaagttc atcatgcaaa agaagaaggt attatattta atacattaac
     2161 aaatcctaag gaaattttag tagatgaaaa tggatatgtt aaaggtatgg tttgtataag
     2221 aatggaatta ggagagccag atgattctgg aagaagaaga cctatagaaa tagaaggttc
     2281 tgaatttgtg cttgatgtag acacagttat aatgtcactt ggaacaagcc caaatccact
     2341 aatatcttct acaacaaaga gtcttgatat aaataagaag agatgcttaa taacagatga
     2401 aaatggtcag acttctaaag agggggtatt tgcaggtgga gatgctgtta ctggagcagc
     2461 aacagtaata tctgcaatgg gtgctggtaa aacagcagca gcatcaatag atgaatattt
     2521 aaaagcaaaa gttaatgctt aatttgtaaa ttaaagagag ggtgtctcaa aatgaacttt
     2581 ttagttcatg aggcactctt tttgtacaaa atcaaatatg ttttaatcat ttcaactatg
     2641 aaaatttcat tcctgtagaa agtctaactt ttaagtctta gtacaatctc tattttgata
     2701 tagccacttt tctagatggt ttaataattt atcagttatt attgtaatat ttgatactaa
     2761 ttaacataac tttgtctaac tatgataaaa tagttataca ataaaggtct tgtaaggagt
     2821 gaataatgtg tggctaataa aatcagattt gtcaaatcta acattaaata atagaaaaat
     2881 aaattgtgta attaatggta ttttaatatg aaaaaaagag aaaaagtatt tgatgggcta
     2941 gtttttaagc atatattaat cttattaata ctattagttt taattgattt aacaagcacc
     3001 atcatatatg caagtaactc tatggaaatt acttctagaa atatgattga ggcatcaaaa
     3061 agagaacttg aaaactattt agaagtaaac atatctttat taaaagctct tagtcaagat
     3121 gatagatttt ctaatggtga aacatcacta attgaaaagg gaaaattatt aaggccatat
     3181 caaaaggaat ataatctttt tatgattggt ataacagata caaaaggaaa tgtttcaagt
     3241 acatataggg aaaatgttaa ctcaataaaa gataagccta gttttgagaa agcaattaaa
     3301 acaaagcaag tggtagtttc agatattgaa gtcagtaatg taacaggtga taaagtattt
     3361 atcatatatg taccaattat aaaaaataat gagatgattg ggaccatttt tgcttctttt
     3421 tattttcaag atgtaaataa tttaataagt agaagcaatt ttgatgattc tattaaattt
     3481 ctaatgatag ataaagatta tactattatt tctcatccaa acaaaaaata tgtaaatgat
     3541 aaaagtaaga ttcttgattt agaagggaat attataggaa ccactaaaag tgaaatttta
     3601 aaaaatataa ataaaaaatg tcaaggtaat tttttatctt gggataattg gagactatat
     3661 aatgtaaaat acacgaatat aaagtggacc aattggactt tagtatcaaa atgtaatata
     3721 tttaagaact tcaaaaatct aatagttaat tttatgataa agttgtattt ttatatagta
     3781 atatttatga tattgtggaa attaagtaat gctaaattaa tagaacaact taaaaaattg
     3841 gcttattatg attctttaag tggaataaaa aataaagaaa agtttagaaa agattctatg
     3901 tatattttaa agaattatta tcaagataat ttttacttag tacaattgga tgtcaacaaa
     3961 tttaaatata taaatgagat gtttggatat tctgaaggga ataagatatt aattcatata
     4021 tcacaggttt taaacaataa tacaaataaa tatgaaattt gcgctaggat ggataatgac
     4081 cattttatat tgttgattgc ttgtaacact gaagatgaac ttttaaatag attatctaaa
     4141 ataaataaag aaatttgtaa cttaattact actaactcat ctaagtataa aattgttatg
     4201 tcaagtggaa tatataagat tactaaaaag gatgatatta aaaagattga cttgttaatt
     4261 gatagagcaa atattgcagc aaaaagtaaa aaggaaaaat atgaacattc atattcgttt
     4321 tttaatgaag atacaagaaa tcgattgtat aaggaaaaac gattggaaga taatatgaat
     4381 aaagctttag aaaaaggtga atttatagtg tattatcaac ctaagtatag tttaggtgat
     4441 gtaaatgaaa ttgaaggagc agaagccttg ataagatgga atagtccaga atttgggttt
     4501 atttcaccta tagattttat acctttattt gaaaagaatg gttttattgt aaatattgat
     4561 atgttcgtgt ttgaagaagt atgcaaaacg ttaaacaagt ggataaataa agggtatact
     4621 cctgtaccaa tctctgtaaa tatgtctaga gttcatttat atagggacaa ttttatagaa
     4681 aatataactg atttgataag taaatacaat atatctcctg agtttattga acttgaacta
     4741 acagaaagtg ttgtatttga taatttaaat atattgattg atataatgaa aaagattaaa
     4801 gagataggat tccttatatc aatggatgat tttggttcgg gatattcatc tttaaatctg
     4861 ttaaaagacc tttcttttga tattttaaaa ttagatagag gatttctaat tgaaacaaca
     4921 gatacaaaac gtggaaaaat tattatttct aagattgtgg aaatggcaaa agcgattgat
     4981 attaaagtaa tctgtgaggg cgtagaaacc tatgaacaag tagagttctt gaaagagata
     5041 ggttgtgata aggttcaagg atacttattt gctaaaccta tggtattaga tgaatttgaa
     5101 aagcacttaa actttaaatt tgattaatca ttaataattc ttagactatg agatacccta
     5161 acaacttact tacaaatatg taagttaatt gttagggtat ttttatggtt aaagtagtat
     5221 attcacgata ttatggtatt gtagtaaaaa taaaaaatta tggagaatat aaaatgaaca
     5281 tattgagtat tagaaatata tcaaaaacat attgtggaaa tattccattt aaagcattag
     5341 acaaggttag tctaaatata gaaaagggag aatttgtgtc tgttatggga ccatctggaa
     5401 gtggtaaatc tactctttta aatataatat ctacagttga tagacagagt gaaggtgagg
     5461 ttatattaga tggatatgat gtaagtaaat tagaaggaga aaaacttgct gaatttagaa
     5521 ggaaacaact tggttttgta tttcaggatt ttaatttaat tgacacttta actgttggcg
     5581 aaaatattat gttgccactt acactagatg gggcaagtat caaagatatg aatatacaaa
     5641 ctaaaagtat atctaaattt ttaggaatag ataaaatttt agatagaaaa acctatgaaa
     5701 tttcaggagg gcaagctcaa aggtgtgcta ttgctagagc tattataaat aaacctgcca
     5761 tacttcttgc agatgagcca actggaaatc ttgattcaag atctactgat gatgttttga
     5821 aattatttac taggataaat aaagaacaaa atgtaacaac acttatggta actcatgaag
     5881 catatagtgc aagttattca gatagagtaa tttttataaa agatggttgt atatatacag
     5941 aaataaaaaa atctgaaagc agtaattctt tctattctga tatattagct gtattgtctc
     6001 aaataggagg tgtaagatag tggattttag aaagtttgct tataataatg tagttagaaa
     6061 ttttagagca tatttagcat attttttaag ttctgttttt tctataatga tattttttgt
     6121 ttttgcagtg tctatgttcc atccaattat aacaaaatct ggaattcaaa gtggttctac
     6181 tgcatttatg gctcttatga cttcagaatt aataatatta ggttttagtc tactttttat
     6241 attgtactct ttaggaaatt tcttaaaata tagattgaaa gattttggag tattgatgat
     6301 aattggaatg agtaacagac aactgaaaaa attgatatta attgaaaatt tgataatagg
     6361 gtttttagca gtgatgttag gaatattact tggtttatca atttccaaat tgtttttatt
     6421 gtatttaagt aaactgtttt atatgaattt aactgaattt tattttccag taaaagcaat
     6481 actattgact attgtatcat ttatgatttt gtttttaata acaggaccaa ttagcttaaa
     6541 attattgagt aaaaagagca ttttagagct tttaataggt acaaaaaagc ctaaaaaaga
     6601 gataaaatct tcaaagctat ttggaataat tggtgcagtg tgtcttatat taggatatgc
     6661 tatgataata tttggcaata agttaaaaat aaatggaggt atgtatacaa cagcactatt
     6721 aattactttg gggatatttt tatttttttc tcaatttacg attttgatat taaagtattt
     6781 aaaaaataac gaaaaatttt ataagaacaa agtaaatatg ttacttataa gtaatttgat
     6841 atataaatta aaggataata cacgattgtt atttttaata acaatacttc tatcggctac
     6901 tttagtagca tttacaacaa cttcaacact tgtaagttct caaggagaat cttctaaaaa
     6961 taatttccca atggtttact catatttttc agcgcataat aatgaaaaag aatatgaaga
     7021 actacaacaa atcagagatg caatcaaagg atatgattat aaggagttaa gttttcctgt
     7081 acttaaatac aatagggaag tcttactaag tgtaaagaat tataacaaat tatcaaagga
     7141 attggggtta gataaaatta gttcaaagtt agaaaaatat gaaggaatta taattcccag
     7201 tagcatttcg caagaacata taaattcttt aaaacaatta aaaaattata atataaaaaa
     7261 tttaaatata gaagtaaaaa aatcaacaaa taaaataatt tttcctacag gactgtttag
     7321 taaagtcgta gttataagtg atgacttata caatgaagta aaaggtgaat tttcaaaaat
     7381 taattttcat ggatttgatt ataaaaactg gcaaagcagt gcaaagataa ctgaaaattt
     7441 aaaagaaaaa cattttaatt tggatagaga atataacagg tcatattttt taacattacc
     7501 agatatgtat ttagtagagt tacaacaaag taaaattctt caatttatgg gagtttttat
     7561 aggagtgata ttatttatag cagtttttag ttttttatac ttcagattat atacagatga
     7621 gatagaagat aatgtaaagt acaaaaaact atcaaaaata ggtctatctt ttaaaaatat
     7681 gaataagatt gttagtatag agataggtac attgtttttt ataccgtatt ttatagcaat
     7741 tataaatagc ataatttctt tactgttttt aaatcaagta ttaaataata gtttcaactt
     7801 agaaaatttg ggtatattac tactgatatc atctatatat tcactttatt tttacctatt
     7861 aaaaaaaaat tatactagaa aagtttggtt ttttgattaa agtggtatat aatagatata
     7921 taaattaata tttaaaaagt agttaatatt aggggagggc atttgcacct cccttgtaat
     7981 atgtggaaaa ttgaatatta gaagaaggag gagacttgat ggaaaatcaa caattattag
     8041 gaacagaacg aattagtaaa ttactactaa aatattctat accagctata ataggtatgt
     8101 tggtaaatag tttatataat gtagtagata gaatttttat tgggaatata ccaggggttg
     8161 gaccacttgc aataacaggt cttggtgtaa caatgccaat aatgacaata atacttgcat
     8221 ttgggatgtt gataggtata ggaactacta ctaccatatc aataaaactt gggcaaggta
     8281 aagtagaaga ggcaagaaaa cttataggaa atgcaatgac gttatctgta ataacaggaa
     8341 ttatcataat gatacttgga atattatttg caaataaaat acttacattg tttggagcaa
     8401 gtgaaaatac tttaatatat gcaaaatcat atataaatat tatattatta ggtacagtag
     8461 taaatcttct ttccttttct ttaaaccact caataagagc tgatggtagc cctaaaattt
     8521 cagcaggaat aatgatagta ggatgtttaa ctaatatagt tttagactgg atattaatat
     8581 ttggattcaa tttgggaata caaggagctg caattgctac agtaacttct caagctctta
     8641 cagctatttt aactataggg tattatataa gtggaaaatc aaacttacga ttcagtaaat
     8701 ctaatctaaa attagataaa aagctaataa aagcagtatt tgcaataggt atgtcgccat
     8761 ttgcgatgca acttgcagca agtttagtcc aagtaatatc aaatattgca cttaaaactc
     8821 atggtggaga tttagctata ggggctatgg ctactatttc ttctattgca atggtatttt
     8881 tgatgcctat ctttggaata aatcaaggag cgcaacctat tataggtttc aattatggag
     8941 ctgaaaaata tgatagggtt aaaaaagcat atttaggttc attagtagta gctactataa
     9001 ttttatgtat gggtatggta gtaattatgc ttttcccaga agctattata ggtatattta
     9061 ataaagaccc tgaacttatg aatatatctg ttaatggatt aagaatatac ttacttatgt
     9121 tgccaattgt aggcttatca gttacaggaa caaactttat acaatctata ggaaaagcaa
     9181 aaatggctat gttattgagc cttttaagac aagtaatttt attgatacca gcagtattaa
     9241 ttcttcctac attcttaggt ttacagggtg tatggacagc tcaaccagtg tctgatttta
     9301 tagctacagt aattacaggt atagttgtat ttagagaatt aaaaagatat actcctaaaa
     9361 ctgaaaaatt aaatgaaaat gaaagactaa atgagataac tactgagtaa aaatataaat
     9421 tcagataatt gagcaagaaa ttgcaaaaaa tacaattata tgataaaata aaaccgctgt
     9481 caaggataag agaaattcta attatggagt gattttatgg aatttacaaa gaaaagcaaa
     9541 ttaaaaaata tgtatgtaaa catgaatgta gctagaatgt tgtctaagat aaatgagtat
     9601 aagggtagac aactgcttta caaaaaacaa ccaaaagaaa ttttagaaaa cctagaaaaa
     9661 aaatctttag tagattgttc tgaatctaca tacattggaa atcaaagaga tagtagtaat
     9721 tttaatctag aaaagttgat aagtaatgaa gttacaccta gaagtagaga ggaattgagt
     9781 attgttgaat acagagatgt ggtaaaaact attaacagtg cttatgagag tattcccatc
     9841 agttcacaaa cgattcttga gcttcatgga tacttatata aattttcatc tactagagga
     9901 ggaagttata agtcagataa tgattttatt gaacacaatt taaaacctag tgaatttata
     9961 agtattgatt caagtgtcaa taaagttgaa aaagctgtag aagaaatatg tgaagcttat
    10021 aatacattga tagaggaaga tgaaattgat atattaatat taatttctgc atttgtgtta
    10081 gatttcatat tgatacatcc attcaaagaa ggtaatataa aaatggcgag agtacttatc
    10141 ctattgttac taaataaaaa tgggtatgaa gttggaagat atataagttt aggaaagata
    10201 tttgatgata gttcatatga atattatagt aatttaaatt atttgaaagc ctcaataggt
    10261 agtgaaaaag cagatatgaa tgcatggatt gagtattttt tagagacgat attaactgca
    10321 tatgagaagc tggatgatag tttaaatata tctgataaga aaagacaaac aaaaactagt
    10381 agaatagaga aaataataaa ttctaccctt ggatatttta caaaagaaga tataagagat
    10441 ttatgtcctg acattccaga acctactata aatagagttt ttaataatct aagaaaacaa
    10501 gataagatag aagttgttgc aagaggaaga agtgcaaaat ggaagaaaaa atattaaaaa
    10561 atgtttaaat aactaaaatg gtatagtgtt ttacactata ccatttttag tgtcattagt
    10621 atgagcaata atttaacagt ttaagcaata gcaattttat ttattgatta tttttgaact
    10681 gaaaaatggg gttatatggt atactataat gataattaat tttagatgat aagatataac
    10741 attacagaat atcttaggag tatatgtaaa aaaatataat aaaagtgtga ggtttagtaa
    10801 ttaacaaatt atatggcttc acaccttttt ataaaataaa gatacttagc agaataatat
    10861 tttagttgat atgtttataa ggtggtactt gtttaggtaa aaataaatgg taaaaaagca
    10921 gaagtcatgt gtataaaaac ataagttaca ctctgtaata atagttattt tataattcaa
    10981 caaaagaata gaatgggtat atttgtgaaa taataatata aaataatctt aggagtgagc
    11041 ttatgaagaa aaaattatta tatataaatg taaattcaaa acctgaagac ttatcttcaa
    11101 gtaaaactgt agcaagaaaa tttataaata aatttatgga aaaaaacaaa aattttgagg
    11161 ttgaagagat agacctttat aaagaacata taccgagact tgagtatcag tactttgaaa
    11221 agagaaatag catagttagt gaagaaaatg ctaaaaattt agatgataag gatagaaaag
    11281 agcttacaaa gattagaaat ctttgtgacc aatttgtgag tgcaagtgta tatgtaatag
    11341 cagcacctat gtggagttta tcttttccag caccacttaa agaatatata gattgtataa
    11401 ttcaagatgg aaaaactata tcttttgaag gcaatgataa accacaagga attttaaatg
    11461 atattgatag aagtatggta tatatacaat cttctggtgg acacatacca tgggtgttaa
    11521 agccggttat gaataaaggg ttaaattatg tggaaagtat catgaaattt ataggaatta
    11581 aaaaatttga tgaattatta gtagatggaa ctggaactag tgaagaagaa agacaagctg
    11641 caatagaaaa agcatctgaa aaaatagatg gtataataga tgatatgaaa ttttaattat
    11701 aatattttat taaaaatata aaggaaaaac ctcttttaat gtagaaactt atattgaagg
    11761 aggcgagaat catgcgagaa gaaaaaagta atgaaaagta tgattgttat tggtgtaatc
    11821 aagagaataa tttctgtgta gaaataaaag ataatatagt tatgatagat gatggcactg
    11881 gtacattaaa acaagccgtt ttcataggat ataaacaaat ccaaattaat ttaaattgtt
    11941 cacattgtca aaacttaaat agaataaaat taaatttgta gaattttaaa atggaaatta
    12001 ttgaaggaat taaaaataga aagtgttaat atttagactg aaaattatat tttatacaat
    12061 aaaagtttat ttaaataaca ttgtatttta atatttattt tcagtcttac tttattaaaa
    12121 ttattttttg gttatgtccg gaaaatatct gtctactaat ttttgtgaaa tattttttaa
    12181 ttggtcagcc attgaagtca gacttaataa ggctatcaat aaatcatctt tattttctgg
    12241 atttatacta atattttcaa aattagactc tataaagtct ggtatattct ttctttgatt
    12301 ttcttttatt aacatgaatt tttcgtaaca tgaaactaaa tctttttcac tgaaattgtc
    12361 cttttcatac acaccatgta agattctttt aatttcttgt attgtaagtg tatttttcat
    12421 agaataaata attatcatct gtaaaatttg ttctttagta tacttttttc ccttaactgg
    12481 tttgattaat ccctctttgc tgtaattatt tatcatggtt ttagtaagaa ttttatcact
    12541 ttcaaatctt ttattggcag aaagcttatt gtcaattaga gaaataattt gatccatata
    12601 taagtcaata gagggaatat cgtttgaagt tatatctgca ttattcagtg tttctagtat
    12661 tatagtatta agttcattat tcatcatatg ttacctccat aatatatata atacactaaa
    12721 ttagaaagta aaacaatatt aagacttgta aaaaaaacat aaagtggtat aattaaatat
    12781 ataatatagt aatgaaaact atatttatga atctgatttt taaaatacat aattgtagga
    12841 ggacgaaatg tatcaatttt ttttaaaggg tagagaccca ataagcagtt taactcattt
    12901 tataggtgct tgtttatctc ttttagcaac tataatttta gtttttcaat ctgtttcatt
    12961 acaggaaact tctctcttga tgatagtatc tgtatcggta tttggacttt cactaattgc
    13021 tttatatagt gcaagttcgt attatcattt tttaaagggg acaccagaac aagagttgtt
    13081 ttttagaaaa gtagaccatg caatgattta tgtattgatt gctggctctt atacaccaat
    13141 atgtctgaat tttatggaaa aaaaagaagg cataatattt gttacagcaa tatggatagt
    13201 tgcatttatt ggcataataa taaaaatatt ttggatggat gcacctaggt ggttatctac
    13261 cagtatatat ttacttatgg gttgggcaat tgtttttgat ataaatgcat tcaattcaat
    13321 tcctaaggat tgccttagac tattaattat ggaaggtgta tcttactcta taggtgcaat
    13381 tatttatata attaaaaagc ctaatataag tcctgaattt ggatttcatg aaatatttca
    13441 tatttttatt atgataggtt ctttatttca ctttttagca gttttattgt atgttttata
    13501 gttttaaaag atttgattaa ttgatactaa aattctacaa tgaataaaat ggaactgcct
    13561 tatatagatt atatcttcga caataagaca gttccttatt ataaatatta aattctactt
    13621 ataataaaat tttgataaaa atagtccatt caaaatgcct attccataat tcctttaatg
    13681 tacttttcat ttacgacata tagtttgaat ttatgtacta taattatatc ctctaaattc
    13741 tctgaaacac taacaattct gacacatttg tttctaattt ctacaggtaa atctaaatca
    13801 tttgttaaat tcacatagtc atcatattta tacataaaat catc
//
LOCUS       DAEPXF010000097        13610 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain HB27
            SAMN13376389-rid12479423.denovo.102, whole genome shotgun sequence.
ACCESSION   DAEPXF010000097 DAEPXF010000000
VERSION     DAEPXF010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13376389
            Sequence Read Archive: SRR10518432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 13610)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13610)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001069624.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001069624.1
            Genome Coverage       :: 315x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/15/2021 01:54:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,165
            CDSs (total)                      :: 4,076
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 89
            rRNAs                             :: 10, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 10 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 19 of 69
            Pseudo Genes (incomplete)         :: 44 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 10 of 69
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13610
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13376389-rid12479423.denovo.102"
                     /strain="HB27"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="China: Hebei"
                     /collection_date="2017"
     gene            174..1049
                     /locus_tag="KRL67_002457"
     CDS             174..1049
                     /locus_tag="KRL67_002457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxoguanine DNA glycosylase"
                     /protein_id="HBH1523327.1"
                     /translation="MNVYEKGNGVILEGVTDFDPVHIFECGQCFRWHKQEDGSYTGVA
                     KGRILNVKKENDKIYLNNTNLKEFNSIWYNYFDLGTDYTEIKNKLKNMDEYLNKATEF
                     GWGIRILRQDGWEMLISFIISSNNRIPMIQRAIENLSRKFGKCIGEYEGNEYYAFPTP
                     EELNKASQEEIRACQTGFRDKYIKSTTQAVIENNDKVSEYTNLSTEDCRKELLKFNGV
                     GPKVCDCIALFGMQKYDSFPVDVWVKRVMQEFYIDEDMSLPKMRTYGIDKFKEMSGFA
                     QQYLFYYARELGIGK"
     gene            1067..2557
                     /gene="cls"
                     /locus_tag="KRL67_002458"
     CDS             1067..2557
                     /gene="cls"
                     /locus_tag="KRL67_002458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HBH1523328.1"
                     /translation="MGVIGTIFLFYLIISYLAGAIISVIILLENRDPAKTMSWLLMFI
                     IFPGVGLMIYAISGRNIRKRKLFKTQKLANNIKEKKLFDTLEKITEIVELEKESIKQN
                     KLLRDEEDGSYRKRVINMLLKTGMFPFTKNNKVDVFVDGNEKFKRLIEDIREAKDHIH
                     LEYFIIKDSEIGRVLKEELIKKAKEGIKIRILYDDVGCWRFWFNRKFFREMREVGIEI
                     AAFLPTKFPIIGGKLNYRNHRKIVVIDGIIGYTGGINIGDEYLGKNDKFGYWRDTHIR
                     IKGISVYMLQMTFLIDWYYTTKEVLVTKNYFPSVGNIGESMIQVVASGPDSDWEDIHY
                     AYFSAICQARKNVYIETPYFIPDESLLKAIKSAALSGVDVRIIFPKIADHKIVNIASY
                     SYFEEILRAGGKVYLYNKGFIHSKVVIIDDKIASAGTANMDLRSFMLNFEVNAFIYDE
                     EVIRVMTDDFFEDLSHCEELNLEVFKNRNIIQKIKESVARLFSPIL"
     gene            2750..3034
                     /locus_tag="KRL67_002459"
     CDS             2750..3034
                     /locus_tag="KRL67_002459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012062101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HBH1523329.1"
                     /translation="MKIRPLADRVVIKKVEAEEKTASGIVLPGAAKEQPQIAEVVEVG
                     PGGIVEGKEIKMELTVGDKVIFQKYSGTEVKIEGQEYTILRQSDVLAVIE"
     gene            3067..4695
                     /gene="groL"
                     /locus_tag="KRL67_002460"
     CDS             3067..4695
                     /gene="groL"
                     /locus_tag="KRL67_002460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729227.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HBH1523330.1"
                     /translation="MAKEIKFSEETRRALEAGVNKLADTVKVTLGPKGRNVILDKKFG
                     SPLITNDGVTIAKEIELEDRFENMGAQLVKEVATKTNDVAGDGTTTATVLAQAIIREG
                     LKNVTAGANPILLRKGIQKAVTVAVEELKNQSRIVETQEAISQVASISAGDEEVGKLI
                     AEAMEIVGKDGVITVEESQTMNTELDAVEGMQFDRGFVSAYMVTDVDKMEAVLNDPYI
                     LITDKKISNIQELLPVLEQIVQQGKKLLIIAEDVEGEALSTLVVNKLRGTFDVVAVKA
                     PGFGDRRKEMLQDIAILTGAQVISEELGYDLKEADLSMLGRASSVKVTKESTTIVDGS
                     GDKKSIEDRVTQIKHQVEQTTSDFDREKLMERLAKLAGGVAVVKVGAATEVELKERKL
                     RIEDALNATRAAVEEGIVAGGGTAFVSVIPAIGTLIESLEGEVKLGAQIVKKALEEPL
                     RQIAINAGLEGAVIVQNVVNSEAETGFDALNEKYVNMIEAGIVDPTKVSRSALQNAAS
                     IASTFLTTEAAVADLPEKEDAGMPGMGGGMPGMM"
     gene            4957..5697
                     /gene="larB"
                     /locus_tag="KRL67_002461"
     CDS             4957..5697
                     /gene="larB"
                     /locus_tag="KRL67_002461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel pincer cofactor biosynthesis protein
                     LarB"
                     /protein_id="HBH1523331.1"
                     /translation="MDLRKLLEEVKNENIDIDFALEKLKDLPYEDIGYANIDHHRELR
                     NGYPEVIYCEGKSDEHILGIIDKMNQKSSNILGTRCRKETFLKIKEIYNHAEYEESSK
                     ILKIQNHDIENIGKGKIVIATGGTSDIPVADEAYHTAKFLGNDVDRIYDVGVAGIHRL
                     LNKRHKIDGARVIVAVAGMEGALASVIGGLVDVPVIAVPTSVGYGANFGGLAALLAML
                     NSCASGISVVNIDNGFGAGYLAAMINKL"
     gene            5781..6515
                     /gene="larC"
                     /locus_tag="KRL67_002462"
     CDS             5781..6515
                     /gene="larC"
                     /locus_tag="KRL67_002462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel pincer cofactor biosynthesis protein
                     LarC"
                     /protein_id="HBH1523332.1"
                     /translation="MSEKILYFDIINGISGDMTLASLLNLGVPKEIFLEEIHKLRMSD
                     EFNINISSKTENGIVGTKVEVITKEKHTHRNLVDIFEVIDKSTLNENVKSKAKKIFMV
                     IGEAEAKVHGTTIDKIHFHEVGAIDSIVDIVGASILVDLLCVDKVCASTVSVGSGFVK
                     CEHGVIPIPAPATIEILKGVPIKLNNVNGECTTPTGAAIIKILCDEFVDEFEFSPKQI
                     GYGIGHKKFEVPNILRTVLGEKKKKN"
     gene            6545..6964
                     /locus_tag="KRL67_002463"
     CDS             6545..6964
                     /locus_tag="KRL67_002463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF111 family protein"
                     /protein_id="HBH1523333.1"
                     /translation="MSSEIYSYLYEKILDEKALDIYTESIYMKKNRPATKVSILCNES
                     DLEKFIKILLLETSTFGVRYNAYNREILERKFVKLDTKYGVVTVKLGYYKGELVKATP
                     EYNECKTIAKNEDIPIKKVFADINFRIKEEFSENLLT"
     regulatory      7024..7125
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            7346..8881
                     /gene="guaA"
                     /locus_tag="KRL67_002464"
     CDS             7346..8881
                     /gene="guaA"
                     /locus_tag="KRL67_002464"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000743900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HBH1523334.1"
                     /translation="MKHELVLVIDFGGQYNQLIARRVRENNVYCEILPCTASIERIKE
                     KNPKGIIFTGGPNSAYLEDSPTISKEIFELGVPILGICYGIQIMSHVLGGVVRKGNKQ
                     EKEYGKTAITYGKSSLFEGITTNSVWMSHTDLIEKVPEGFTIVANTNDCPVAAMENVE
                     RNLYGVQFHPEVEHCLEGDKILTNFLYNICKVKGDWTTDSFIEDKIKELKEKIGDKKA
                     LCALSGGVDSSVAAVLIHKAIGDNLTCIFVDHGLLRKNEGNDVERIFREKFDINLIRV
                     NAEDRFLSKLKGVSEPEAKRKIIGEEFIRVFEEESNKLGKMDFLVQGTIYPDVIESGH
                     GNAATIKSHHNVGGIPEDVDFQEIVEPLRELFKDEVRKIGLELGIEEGLIFRHPFPGP
                     GLGIRVIGDVTKEKCDILREADAVYMDELRKAGLYREIWQAFATLPDVKTVGVMGDER
                     TYAYLVGLRAVTSSDGMTSDWYKMPYDVLERISNRIINEVDGVNRVVYDITSKPPGTI
                     EWE"
     gene            9397..10827
                     /locus_tag="KRL67_002465"
     CDS             9397..10827
                     /locus_tag="KRL67_002465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HBH1523335.1"
                     /translation="MTLNQIFIEDGGVMRNKIIIGITFSLVLLMSGCASKQTAEINVL
                     ATTDLHGVIPYELTSYVKEERKKDKNLTLVDAGDFFDSGEVFGSSMDSYFSARYKDGD
                     NNTESYIETPISKDMKEVGYDVAVLGNHEFISNNKFYLDNMISDFEKQKIDILSANTY
                     KKNGDSYTKPYIIKNINTPEGNIKLGILGLTIKEVGERKQWKDGKLVDSKSLELKDQE
                     GYNGELYMNDLVEDAKKWVSIMEKEKPDIIVAVAHTGEKPKKPRNPGNRIQDLAQQVD
                     GIDAIVAGHNHVQIEQHDYKNKSGENVIVTEPGKHGECISKINFKLEKNNDKWNIVDK
                     SSKLIKFEKNQESDKFGEFISSINKVSKDKKEIRLSEVKTPEWDKIYVFKSGTSADDI
                     YKTIGYKWRNISSPKGDDMVQIVVMNNNKPVSYIYGEAEVMPINIKCDESEYKDNVVT
                     IYPNKNDKFKIEKGKQEYSINLKHIQ"
     gene            complement(10975..11850)
                     /locus_tag="KRL67_002466"
     CDS             complement(10975..11850)
                     /locus_tag="KRL67_002466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBH1523336.1"
                     /translation="MINIRYLEILEAIEQTGTFTGAAKKLHITQSAVSHAIAELEQQA
                     ETALFDRLPRGVCLTRCGISLLEEARSILTACRNLDKRISRLEECTPINIVSSITIAS
                     FLLPPILSRLKNSFPSLKVNVRVESANSAMDILKSGEADICFWEGIEPQGAFRTILLG
                     SYKLCVACAPTFPLSEQTLSLRQLCMFPLLLREQGSAIRDTLDSVLSLSNQKAYPVWE
                     SVNSFALIRAAEAGLGITILPENLLLDSLLHKKLRLIELDGIDMENKMLAILHKDKNI
                     TQPLEIILDNISNVL"
     gene            11970..12533
                     /locus_tag="KRL67_002467"
     CDS             11970..12533
                     /locus_tag="KRL67_002467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HBH1523337.1"
                     /translation="MNQSRKTYFWLLIINLFISTFTFGGGYVVVPMIRRYFVNQKQFF
                     TEDDLMSMAAVAQSTPGAIAINLSALAGYRVAGTLGVVISCIAAIIPPLVILGLVSAF
                     YTTFISNAIVTAVLKGMQAGVAALIVDLIIDLCSMILKQRSLFLSVMIPIAFFANFVM
                     SINVIFILSVCCCVCVMQVFYKKRENK"
     gene            12530..13096
                     /locus_tag="KRL67_002468"
     CDS             12530..13096
                     /locus_tag="KRL67_002468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HBH1523338.1"
                     /translation="MNTIWNLFFIFLKIGLFSIGGGYAIIPLIQEKVVNQTGWISEKM
                     FTDIITISQMTPGPLAVNTSTFVGLQIGGFWGAVAATLGCVLCGVVISLFLYNFFQKH
                     QNSTYIFEILNGLKSASLGLIISAAATIIILALFGTNKVYFNSIFEMLNWTALIIFFV
                     TLLIVRKWKINPIVIIIISGILGIFFYL"
     gene            complement(13225..13461)
                     /locus_tag="KRL67_002469"
     CDS             complement(13225..13461)
                     /locus_tag="KRL67_002469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH1523339.1"
                     /translation="MLECFIAEDSKIKDGVNIPTLVFKTLHDRYFKTQYFDSYYNSLK
                     CKKKLVEINNVHNSYYIDSEKFCQEAYDWFVENQ"
BASE COUNT         5309 a         1425 c         2519 g         4357 t
ORIGIN      
        1 cattttttat ttaaggtaaa aatttaaagt aaaatattat tttacataat aataacctta
       61 taaattttga aattacttat ctttgtgtta aaataagtaa aatgcacata ttagagctaa
      121 gtttttaagc aaataaacaa aatgaaaata taaatattaa ggagatttgg tatatgaatg
      181 tttatgaaaa aggtaatgga gttatactag aaggagtaac agattttgac ccagtacata
      241 tatttgagtg tggtcaatgt tttagatggc acaaacaaga agatggctca tacactggag
      301 ttgcaaaggg gagaatatta aatgtcaaaa aagagaatga taaaatatat ttaaataata
      361 ctaatttaaa ggagtttaac agcatttggt ataattactt tgatttagga actgattata
      421 cagaaataaa gaataagcta aaaaatatgg atgaatattt aaataaggct actgaatttg
      481 gatggggaat aagaatatta agacaagatg gatgggaaat gcttatatca tttataattt
      541 cctctaataa taggattcct atgatacaaa gagctataga aaatttatca agaaaatttg
      601 gtaagtgtat aggtgaatat gaaggtaatg aatactatgc attcccaaca ccagaagaac
      661 taaataaggc ctcacaagag gaaataagag catgtcaaac aggttttaga gataagtaca
      721 taaaaagtac tacacaggca gttatagaga ataatgataa agtatctgaa tatacaaatt
      781 taagtacaga ggattgtaga aaagagcttt taaaatttaa tggagtagga ccaaaagtat
      841 gtgattgtat tgcattattt ggtatgcaaa aatatgattc ttttccagtt gatgtttggg
      901 ttaagagagt tatgcaagaa ttttatatag atgaagacat gagtttacct aaaatgagaa
      961 cttatggaat cgataaattt aaagaaatgt ctggatttgc tcaacagtac ttattttact
     1021 atgcaagaga actcggtata ggaaaataaa ttaaggggga gaaattatgg gagtaatagg
     1081 aacaatattt ctgttctatt taattatatc atatctagct ggagccataa tttctgtgat
     1141 tatattactt gaaaatagag accctgcaaa aacaatgtct tggttattga tgtttattat
     1201 atttccaggt gtaggtttaa tgatatatgc tatttcaggt agaaatatta gaaaaagaaa
     1261 attatttaag actcaaaaac tagctaataa tataaaagaa aagaaactat ttgatacatt
     1321 agaaaagata acagaaatag ttgaattaga aaaagaatct ataaaacaaa ataagctttt
     1381 aagagatgaa gaggatggta gttatagaaa aagggtaata aatatgttat taaagactgg
     1441 tatgtttccc tttactaaaa ataataaagt ggatgtgttt gtagatggaa atgaaaagtt
     1501 taaaagactt atagaagata taagagaagc taaagaccat attcatttag agtactttat
     1561 catcaaagat agtgaaatag gtagggtgtt aaaagaagaa cttataaaaa aggctaaaga
     1621 aggcattaag ataagaattt tatatgatga tgtaggatgc tggagatttt ggtttaatag
     1681 aaaatttttc cgagaaatga gggaagtagg tatagaaatt gcagcatttt tacctacaaa
     1741 atttcctata ataggaggga aattaaacta tagaaatcac agaaaaattg ttgttattga
     1801 tggaataatt ggatatacag gtggaataaa tataggagat gaatatttag gcaaaaatga
     1861 taagtttgga tattggagag atactcatat taggattaag ggtatttctg tatacatgct
     1921 tcaaatgaca tttctgattg attggtatta tacgacaaaa gaagtattag taactaaaaa
     1981 ttattttcca agtgtaggga atattggtga aagtatgata caagttgttg cgagtggtcc
     2041 agacagtgat tgggaagata ttcattatgc ttatttttct gcaatatgtc aggctagaaa
     2101 aaatgtttat atagaaactc catattttat tccagatgaa agtctattaa aagctataaa
     2161 gagtgctgct cttagtggag ttgatgtgag aataatattt cctaaaattg cagaccacaa
     2221 gatagtaaat atagcatctt attcatattt tgaagaaata ttgagagcag gtggtaaagt
     2281 ttatttatat aataaaggat ttatacattc taaagtcgta ataatagatg ataaaattgc
     2341 ttctgcaggt acagctaata tggatttaag aagttttatg cttaactttg aagtaaatgc
     2401 atttatctat gatgaagaag ttattagggt aatgacagat gatttctttg aggatttaag
     2461 tcactgtgaa gaacttaatt tggaggtatt taagaataga aatatcatac aaaaaatcaa
     2521 agaatctgta gcaagattat tttcaccaat attataaaaa attattaaaa aaggtattga
     2581 aaattatgtg tgattggtat attataataa ttgttagcac tctattaggt agagtgataa
     2641 taaataaaaa gtatatatat aaatctacat atataagtag tattaattta aaaataacta
     2701 aaaacatagg cgaactagtc tatacttaat attttaggag gcgtgaataa tgaaaataag
     2761 accattagct gacagagtag taattaaaaa agtagaagca gaagaaaaaa ctgcaagtgg
     2821 aatagtttta ccaggagcag ctaaagagca acctcaaata gctgaagttg tagaagttgg
     2881 accaggtgga atagttgaag ggaaagaaat aaaaatggaa ttgacagtag gagataaggt
     2941 tatattccaa aaatattctg gaacagaagt taagatagaa ggacaagaat atacaatact
     3001 aagacagagt gatgtattag ctgttattga ataaatatag aataaattta ttaggagggg
     3061 tttaaaatgg ctaaagaaat taaattttca gaggaaacaa gaagagcatt agaagctggt
     3121 gtaaataaat tagcagatac agtaaaagta acattaggac ctaaaggaag aaatgttata
     3181 ttagataaaa aatttggttc tccacttata actaatgatg gagtaactat agcaaaagag
     3241 atagagttag aagatagatt tgaaaatatg ggtgctcaat tagttaaaga agtagcaact
     3301 aaaactaatg atgtagctgg agatggtact acaactgcta cagttttagc acaagctata
     3361 ataagagaag gtttaaagaa tgtaacagca ggggctaacc caatactttt aagaaaagga
     3421 atacaaaaag cagtaacagt agcagtagaa gaattaaaaa atcaatcaag aatagtagaa
     3481 acacaagagg ctatatctca agttgcttct atatctgctg gtgatgaaga agttggaaaa
     3541 ttaatagcag aagctatgga aatagtaggt aaagatggag ttataactgt tgaagagtct
     3601 caaactatga atactgagtt agatgctgtt gaaggtatgc agtttgatag aggttttgtt
     3661 tctgcatata tggttacaga tgtagataaa atggaagcag ttttaaatga cccatatata
     3721 ttaattactg ataaaaaaat atctaacata caagaattat taccagttct tgaacaaata
     3781 gttcaacaag gtaaaaagct attgataata gctgaagatg tagaaggtga agcattatct
     3841 acattagtag taaataaatt aagaggaaca tttgatgtag tagcagttaa agctccaggc
     3901 tttggagata gaagaaaaga aatgcttcaa gatatagcaa tacttacagg tgctcaagta
     3961 atatcagaag aattaggtta tgatttaaaa gaagctgatt tatctatgtt aggtagagct
     4021 tcatctgtta aggtaactaa agaaagtact acaatagtag atggttctgg agataaaaaa
     4081 tctatagaag atagagtaac tcaaattaaa catcaagtag agcaaactac ttcagatttt
     4141 gatagagaaa aattaatgga aagattagct aaacttgctg gaggagtagc tgttgtaaaa
     4201 gttggagctg ctacagaagt agagttaaaa gaaagaaagt taagaataga agatgccctt
     4261 aatgcaacta gagcagctgt agaagaagga atagtagctg gtggaggaac tgcttttgtt
     4321 agtgttatac cagcaatagg aacactaatt gagagtttag aaggagaagt taaactaggt
     4381 gctcaaatag ttaaaaaagc gttagaagag ccattaagac aaatagctat aaatgctggt
     4441 cttgaaggtg ctgtaattgt gcaaaatgtt gttaattctg aagcagaaac tggatttgac
     4501 gcattgaatg aaaaatatgt aaatatgata gaagctggta tagttgaccc aactaaagtt
     4561 agtagaagtg ctttacaaaa tgctgcatca atagcaagta ctttcttaac tactgaagct
     4621 gctgttgctg accttcctga aaaagaagat gcaggaatgc caggaatggg tggcggaatg
     4681 ccaggtatga tgtaatttaa aaaatttaaa atataaattt taatataaag aactatttcg
     4741 acttagattt taaactctaa gttgaaatag ttcttttgta tatatctaat ttttgtatat
     4801 ttataaattc aatacacata tttagataat cagtatctag tttgtaataa tattttagag
     4861 tagtattgtt attttttagg ttatgatttt agatatatat taattaaggt ataattgttg
     4921 atagagaata aatcaaattt aaaaggatgc gataatatgg acttaagaaa attgcttgaa
     4981 gaagttaaaa atgaaaatat tgatatagat tttgcgttag aaaaattaaa agatttacct
     5041 tatgaagaca ttggatatgc aaatatagac catcatagag aattaagaaa tgggtatccg
     5101 gaagttattt attgtgaagg taagagtgat gagcatattt tagggattat tgataaaatg
     5161 aatcaaaaaa gttctaacat attggggaca aggtgtagaa aagaaacttt tctaaaaatt
     5221 aaagaaatct ataatcatgc tgagtacgaa gagtcgtcta aaattcttaa gattcaaaat
     5281 catgatatag agaatatagg aaaaggtaaa attgtgattg ctacaggggg aacttctgat
     5341 ataccagtag cagacgaagc ctaccataca gctaagtttt taggcaatga tgttgataga
     5401 atatatgatg ttggagtggc aggtatacat aggcttttaa ataaaagaca taaaatagat
     5461 ggtgctagag taatagtagc tgttgcaggt atggaaggtg ctcttgcaag tgtaattggt
     5521 ggactggttg atgttccagt aatagctgtt ccaacttctg ttggatatgg agcaaatttt
     5581 ggaggattag cagccttact agctatgtta aatagctgtg catctggaat atctgttgta
     5641 aatattgata atggatttgg agcaggatat ttagcagcaa tgataaataa attgtaaata
     5701 cattgatata aattaaacaa aactatataa taaaatttaa tttctattat atttttacta
     5761 ttaaaattga ggagacatat atgagtgaaa aaatacttta ttttgatata ataaacggta
     5821 tatcagggga tatgaccctt gcaagtttac taaatttagg tgtacctaag gaaatttttt
     5881 tggaggaaat tcataaacta agaatgagcg atgaatttaa tataaatatc agctcgaaaa
     5941 ctgaaaatgg aatagtaggt acaaaagtag aagtaattac aaaagaaaaa catacacata
     6001 gaaatttagt ggatatattt gaggttatag ataaaagcac tctaaacgaa aatgtaaaat
     6061 ctaaagcaaa aaaaatattt atggtaattg gagaagcaga agcaaaagtt catggaacta
     6121 caatagacaa gatacatttt catgaggtag gggctataga ttcaattgta gatatagtag
     6181 gagcttctat attagttgac ttactttgtg tagataaagt ttgcgctagt acggtgtctg
     6241 ttggttcagg atttgtaaaa tgtgaacatg gagttatacc tatacctgca cctgcaacta
     6301 tagaaatttt aaaaggtgtg ccaattaaat taaacaatgt aaatggagaa tgtacaactc
     6361 caacaggagc tgcaatcata aaaattttgt gtgatgagtt tgtagatgag tttgaatttt
     6421 ctccaaagca aattggatat ggtataggtc ataagaagtt tgaagtgcca aatatactca
     6481 gaactgtatt aggtgaaaaa aaaaagaaga attaatttat gaaattagtg caaacattga
     6541 tgatatgtct tcagaaatct attcttattt gtatgaaaaa atattagatg agaaagcatt
     6601 agatatttat acagaaagta tttatatgaa gaagaataga ccagcaacaa aagtatcaat
     6661 tttatgtaat gaatcagatt tagaaaaatt tattaaaata ttacttttag aaactagtac
     6721 ttttggagtg agatataatg catataacag agaaattcta gagagaaaat ttgtaaaatt
     6781 agatacaaaa tatggagtgg ttacagtaaa attagggtat tataaaggag aactagtcaa
     6841 ggcgactcca gaatataatg aatgtaaaac tatagcaaaa aatgaagata ttcctataaa
     6901 aaaagtgttt gctgatataa attttagaat aaaagaagaa tttagtgaaa acttattgac
     6961 ataagtaata aattttgata aattaatatt cagccttatt aaataaaagt aaacagaaaa
     7021 aaatatataa aatataaact cactcgtata tgctcagaaa tatggtctga gagtctctac
     7081 caagataccg taaatatctt gactatgagt gaaattatta taccaaaaga cctagttaag
     7141 taaatgtgta ggctgagttg tactccctat gtgttggcta taagataaag gtattttgga
     7201 agcctgtata gtaatttcac ttttgctaga aattatggaa gttactatac aggttttttg
     7261 tgtaaaaaga tttaatataa tttagactgt attgtgttaa aataaatata taaaaactat
     7321 attatacaag gagagatgtg aatttatgaa acatgaatta gtacttgtaa tagattttgg
     7381 agggcaatat aatcagttga tagctagaag agttagagaa aacaacgtat attgtgaaat
     7441 acttccatgt actgcaagca tagaaagaat aaaagaaaaa aatcctaaag ggataatatt
     7501 tacaggtggt ccaaatagtg cgtatttaga agattctcca acaatatcaa aagaaatatt
     7561 tgaactagga gtaccaatac ttggaatatg ctatggtatt caaataatgt ctcatgtatt
     7621 gggtggagtt gtaagaaaag gtaataaaca agaaaaagaa tatggaaaaa cagctataac
     7681 ttatggaaaa tcatcattat ttgaaggaat aactacaaat agtgtatgga tgagtcatac
     7741 agatttaata gaaaaagttc cagaaggatt tactatagtt gcaaatacta atgattgtcc
     7801 agtagcagct atggaaaatg tagaacgaaa cttatatgga gttcaattcc atccagaggt
     7861 agaacactgt ttggaagggg acaagatact tacaaacttc ttatataata tttgtaaagt
     7921 aaaaggtgac tggactacag attcattcat agaagataaa ataaaagaat taaaagaaaa
     7981 aataggcgat aaaaaagctt tatgtgcttt aagtggagga gttgattctt ctgttgctgc
     8041 agttcttatt cataaagcaa ttggggacaa tttaacttgt atatttgttg accatggttt
     8101 acttagaaaa aatgaaggaa atgatgtaga aagaatattt agagagaaat ttgatataaa
     8161 tttaataaga gttaatgcag aagatagatt cttatctaaa ttaaaaggag tttctgaacc
     8221 agaagctaag agaaaaataa taggtgaaga atttataaga gtatttgaag aagaatcaaa
     8281 taaattagga aaaatggatt tcttagtaca aggaacaatc tatccagatg ttatagaaag
     8341 tggacatgga aatgctgcta ctataaagtc tcatcataat gttggtggaa tcccagaaga
     8401 tgtagacttc caagaaattg tagaaccatt aagagagtta tttaaggatg aagttagaaa
     8461 gataggatta gagcttggta tagaagaagg tttaatattt agacatcctt tcccaggacc
     8521 aggtctagga ataagagtta taggtgatgt aactaaagaa aaatgtgata tattaagaga
     8581 agctgatgct gtttatatgg acgagcttag aaaagcaggt ttatatagag aaatatggca
     8641 agcttttgca acattaccag atgttaagac tgttggagtt atgggtgatg aaagaacata
     8701 tgcttattta gttggtttaa gagcagttac ttcttctgat ggaatgactt ctgactggta
     8761 taagatgcct tatgatgtat tagaaagaat ttctaataga ataataaatg aagttgatgg
     8821 agtaaataga gtagtttatg atataacttc taaacctcct ggaactattg aatgggaata
     8881 aagtatagaa tgccaaaaat ccacatgaat acaggatttg aggtctctct tacttctata
     8941 ttatactggt ttgatactat caatatttgc cagtatctta tggattaata agcaaaaaac
     9001 atcctgctga atgataaatt cagcggggtg tttttatttt gttttggaac tcttttgctc
     9061 tgaaactcga agtatttaac actgatgatg ttgttatctt ttacttcctt ttgtgtcatg
     9121 ctctgaaaga caaattgata caataaaaaa gtaatttctt aatcattgat ggatatatgt
     9181 atttatgtta cttttgatgg taatttgagt aatattgttc atagcttttt atattcactt
     9241 aattaaataa acttgatgat ataatataaa agatttataa ttattagaag tatataaaag
     9301 tattttatgt ctatgaaatg tatgtctata aaacatataa atgacttttg tatattaatg
     9361 tatgaatatt gatattatag aagaattgag ttgtcaatga cactaaatca aatatttata
     9421 gaagatggag gggttatgag aaataaaata attataggaa ttactttttc acttgtatta
     9481 ctaatgtctg gatgtgcttc aaaacaaaca gcagaaataa acgtattagc aactactgat
     9541 ttacatggag ttatacctta tgagttaact tcttatgtaa aagaagaaag aaaaaaagat
     9601 aaaaatctaa ctttagtaga tgcaggagat ttttttgata gtggagaagt gtttggttct
     9661 agcatggata gttattttag tgctagatat aaagatggtg ataataacac tgaaagttat
     9721 attgagacgc ctatttcaaa agatatgaaa gaggtaggtt atgatgttgc agttcttggg
     9781 aatcatgaat ttatatccaa caataaattt tatctagata atatgatatc tgattttgag
     9841 aagcaaaaaa tagatatttt atcagcaaat acgtataaaa aaaatggaga tagttataca
     9901 aaaccataca taataaaaaa tattaataca cctgaaggaa acataaaact aggaatttta
     9961 gggcttacta taaaagaagt tggtgagagg aaacagtgga aagatggaaa acttgttgac
    10021 tctaagtctt tagaactaaa agaccaagag ggctacaatg gtgaattgta tatgaatgat
    10081 ttagtagaag atgctaaaaa gtgggttagt ataatggaaa aagagaagcc tgacattata
    10141 gttgctgtgg cacatacagg tgagaaacct aagaaaccta gaaacccagg aaatagaata
    10201 caagacttag ctcaacaagt tgatggaatt gatgctatag ttgcaggtca taatcatgtt
    10261 caaattgagc aacatgatta taaaaataaa tcaggagaaa atgttatagt tactgagcca
    10321 ggaaagcatg gagaatgtat ttctaagatt aattttaaat tagaaaaaaa caacgataaa
    10381 tggaatatag ttgacaaatc tagtaaatta attaaatttg aaaaaaatca agaaagtgat
    10441 aaatttggag aatttatttc ttcaattaat aaagtaagta aagataagaa agaaataaga
    10501 ttatcagaag taaagactcc tgaatgggat aaaatatatg tttttaaatc tggaacatct
    10561 gcagatgata tatataaaac aataggatat aaatggagaa atatatcatc tccaaagggt
    10621 gatgacatgg tgcagattgt tgtaatgaat aataataagc ctgttagtta tatatatggt
    10681 gaagctgaag ttatgccaat taatataaaa tgtgatgaat cagagtataa agataatgtt
    10741 gtaactattt atcctaataa aaatgataaa tttaaaattg aaaaaggtaa acaagaatat
    10801 agtattaatt taaaacatat acaatagaaa cttttatctt atttaagttt agattgtact
    10861 taaaataaag tgtgtattat tttaaaaagt gatgataatt aaaatcacaa tattaaggaa
    10921 tagtacacac tttttatgtt aaaaaataga atgttaaaaa attaaacttt taaattaaag
    10981 tacattgctt atattatcca atattatttc caaaggttgt gtaatatttt tatccttatg
    11041 taatattgct aacattttat tttccatatc tatcccatct aattcaatca atcgcaattt
    11101 tttatgtaag agtgaatcta ataataaatt ttctggtaaa atggttatac ctaatcctgc
    11161 ttctgcagct ctaataagtg caaaagaatt aacactttcc caaacaggat aagctttttg
    11221 gtttgaaaga gataatacac tgtccaaagt atcgcgaata gcacttcctt gctcacgtaa
    11281 caataacgga aacatgcata actgtcgtag agataaggtt tgttcagata aaggaaaagt
    11341 tggagcacat gcaacacata acttatagga acctaataaa attgtacgga atgccccctg
    11401 tggttctata ccttcccaaa aacatatatc agcttctcca ctttttaaaa tatccatagc
    11461 tgaattagca ctttcgacac gtacattcac tttaagagat gggaatgaat tttttagtcg
    11521 acttaaaatt ggtggaagta aaaaagaggc aattgtaatg ctagaaacaa tgtttatagg
    11581 tgtacattcc tctaaacgac tgattctttt atctaaattg cgacaagcag ttaagatact
    11641 acgtgcttct tctaaaagag aaatgccaca gcgagtaaga catacccctc gtggtagtct
    11701 gtcaaataat gctgtttcag cttgttgttc aagttcagct atagcatgag atacagcaga
    11761 ttgtgtgata tgtaattttt ttgcagcacc tgtaaaagtg cctgtttgtt caattgcttc
    11821 taaaatctct aaatatcgta tgtttatcat aataaatatc ctcactagac atgaataaaa
    11881 ttgattaatt ccattaaatt ctatcatttt acagattgca aagcaacagg tatactgaat
    11941 aatatgaagt gaaaagaagg aggaatattt tgaatcaaag cagaaaaaca tatttttggc
    12001 ttttaattat taacttgttt attagcacat tcacttttgg tggagggtat gtagttgtac
    12061 ctatgattag acgttatttt gtaaatcaaa agcaattctt tacagaagat gatttgatga
    12121 gcatggcagc agttgcacaa tcaactcctg gagctattgc aattaattta tctgctttgg
    12181 caggttatcg agtagctggt actttagggg ttgtcattag ttgtatagca gctataatac
    12241 ctccactcgt tattcttgga ttagtatcag cattttacac cacttttata tctaatgcca
    12301 ttgtaactgc agtattaaaa ggaatgcaag ctggtgttgc tgcactaatt gtggatttga
    12361 ttattgactt gtgctctatg atattaaaac aacgttctct atttttatca gttatgatac
    12421 ctattgcttt ctttgcaaac tttgtgatga gtataaatgt aatttttata ttatctgttt
    12481 gctgttgtgt atgtgtaatg caagttttct acaagaagag ggaaaataaa tgaatacaat
    12541 ttggaactta ttttttatat ttttaaaaat aggattgttt agtattggtg gtggttatgc
    12601 aatcatacct ttaatccaag agaaagtagt aaatcaaact ggatggataa gtgagaaaat
    12661 gtttacagat attataacta tttcacaaat gacaccaggg ccactggctg ttaatacttc
    12721 tacttttgtt gggcttcaaa ttggaggttt ttggggagca gttgcagcca ctttaggttg
    12781 tgtattatgt ggtgtagtaa tatctctatt tttatataat ttttttcaaa agcatcagaa
    12841 ctctacttat atatttgaaa tattaaatgg tctaaaatca gcttcattag gtttaattat
    12901 ctcagctgcg gctacaatta taatccttgc attatttggg acgaataaag tgtacttcaa
    12961 ttctatattt gagatgctaa actggactgc actaataatc ttttttgtta cactactgat
    13021 agtacgtaag tggaaaatta atcctattgt gattataata ataagtggta tactgggaat
    13081 atttttttat ttataagttt ttgctaagta aaggatatct atttttttat atcttaaatc
    13141 caaatagcca ataaagtaag gctatttgga tttttaataa attctgaaaa gataatcaga
    13201 aacccatata acatttaaat tatatcactg gttttctaca aaccaatcat aagcttcttg
    13261 gcaaaatttc tcactatcta tatagtaact attatgaaca ttattaattt caacaagttt
    13321 ctttttacat tttagtgaat tataatagct atcaaaatat tgggttttaa aatatctatc
    13381 atgaagtgtt ttaaagacaa gagtaggtat gttgactcca tcttttatct tactatcttc
    13441 tgcaataaag cattctaaca ttgtattaat tatctttgta ctatttttcc aaatatgagg
    13501 atttttcttt tcaaatattt tataaaattc attatcatag acttcattat aacctttata
    13561 tttaggtggt tttactgtta tgtgaaaatt aggaagaaac ttaaagaaaa
//
LOCUS       DAEPXF010000098        13589 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain HB27
            SAMN13376389-rid12479423.denovo.103, whole genome shotgun sequence.
ACCESSION   DAEPXF010000098 DAEPXF010000000
VERSION     DAEPXF010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13376389
            Sequence Read Archive: SRR10518432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 13589)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13589)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001069624.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001069624.1
            Genome Coverage       :: 315x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/15/2021 01:54:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,165
            CDSs (total)                      :: 4,076
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 89
            rRNAs                             :: 10, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 10 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 19 of 69
            Pseudo Genes (incomplete)         :: 44 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 10 of 69
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13589
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13376389-rid12479423.denovo.103"
                     /strain="HB27"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="China: Hebei"
                     /collection_date="2017"
     gene            403..633
                     /locus_tag="KRL67_002470"
     CDS             403..633
                     /locus_tag="KRL67_002470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HBH1523340.1"
                     /translation="MSNKKKKYANILANECVACGSCIKACPRSAISVPCGISAKINRD
                     LCVGCGICEKICPASVIEIITILKEEEGKCHE"
     gene            626..1207
                     /locus_tag="KRL67_002471"
     CDS             626..1207
                     /locus_tag="KRL67_002471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HBH1523341.1"
                     /translation="MNNKKHWYDYLWIFSSIYLILGFVNIIFAWIGLICFFVPLAISI
                     VKGNKAYCNKYCGRGQLFNLLGNKLKLSRNRDIPKFIRSKWFRYGFLTFFMVMFANML
                     FSTYLVFEGSRNFKEVVTLLWTIKMPWQWAYHGTLVSPWVVQFAFGFYSVMLTSTVLG
                     LITMMLFKPRSWCVYCPMGTMTQMICKAKSKSV"
     gene            complement(1766..2443)
                     /locus_tag="KRL67_002472"
     CDS             complement(1766..2443)
                     /locus_tag="KRL67_002472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HBH1523342.1"
                     /translation="MISQNEIDILTHSLPFWDKLTDLQKELLISSANTSHYKKGNPVH
                     CGDSDCIGILIIKSGTVRTYILSDEGREVTLFRLDDGDVCILSASCILKTITFDVYVD
                     AETDCDIIQISSSVFAKLSTENIHAELFSYKLATERFSDVMWAMQQILFMSFDKRLAS
                     FLIDEIAKNGSSTINMTHEQIAKYMGSAREVVSRMLKYFAREGIVSLSRGGIKVLDKD
                     KLRSLTL"
     gene            complement(2632..3996)
                     /locus_tag="KRL67_002473"
     CDS             complement(2632..3996)
                     /locus_tag="KRL67_002473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HBH1523343.1"
                     /translation="MKESNKRIELLGSAPISKALLSMGLPTMIGMMITALYNLVDAYF
                     VGGLGTSQMGAISVAFPLGQLVVGLGLLFGNGAASYIARLLGHGDNEKAGKVVSTSIY
                     SSIIVGTLVIIVIMIFLNPILKLLGATKSILPYAISYASIYVVSSIFNIFNVTMTNIV
                     TSEGAAKTTMCAMLLGALLNMILDPIFIYTFGMGVRGASIATAISQIASTLVYIGYIL
                     SKKSIFSFRISECCFSKEIFSEILKIGIPTLIFQLLTSLAITLTNMEATKYGDSVIAG
                     MGAVTRITSLGSLIVFGFIKGFQPIAGYNFGSKNYDRLHIAIKTSILWTTIFCVMLGL
                     ILVLFPKSIISQFTSGDILLIENGSKALRANGFSFMLFGFYTVYSTLFLALGKAKEGG
                     FLGMCRQGICFIPIILIIPVFYGLNGILYAQPIADLIAAIIAGFMSIRLHKELNLLKI
                     NCMH"
     gene            4124..4645
                     /locus_tag="KRL67_002474"
     CDS             4124..4645
                     /locus_tag="KRL67_002474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HBH1523344.1"
                     /translation="MATIDLIVLGIVKQQPKSAYDIQKAVEYRNISRWVKISTPSIYK
                     KVIQLEQKGYIESKTVKNGKMPEKAVYSLTELGETHFLDLMFDTSSRPINILLDFNAV
                     IVNLNSLTPEKQKLCLNNIEENVQELKKTIEENISIKTEVPETAKAVLEQQLVLAQAL
                     ETWINSLKDNMGN"
     gene            5025..6104
                     /locus_tag="KRL67_002475"
     CDS             5025..6104
                     /locus_tag="KRL67_002475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HBH1523345.1"
                     /translation="MPELLKNMYNRESLYEVAVAIQSVYNSFKVDEFIKYTMDETWNN
                     LELKARCRKISMSLGMYLPEDYKEALSILEKSVAGFYFAFFFPDFVEVYGQDDVNWDL
                     SISALERNTEYWSSEFAVRAFIIKDEERMMAQMRKWSKHKNEHVRRLASEGCRPQLPW
                     GQAIAKFKKDPTPVLPILEQLKTDTSTYVQKSVANNLNDISKTHPDLVVSIAKDWYGK
                     NKSTNWIVKHGCRTLLKKGNRDVLALFGYDDATSINIQDFTLETTSISIGEDLTFSFN
                     ILAKKATKTRLEYGIDYVKFNGKRNRKIFKISEVSLKENEKKSYMKKHSFADGSVRRH
                     YPGIHSITIIINGVEKGKLDFELGV"
     gene            6427..6795
                     /locus_tag="KRL67_002476"
     CDS             6427..6795
                     /locus_tag="KRL67_002476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HBH1523346.1"
                     /translation="MNVYSFWNDTLAQDAVKIRKYFHEDAYINWHDTNEQFTLEEFII
                     ANCEYPDKWNGKVERVLEFGNLVITVTHVYAINKPLSFHVVSFISLKDDKIISIDEYW
                     GEDDDVPQWRLDKHIGKPIK"
     gene            6912..7715
                     /locus_tag="KRL67_002477"
     CDS             6912..7715
                     /locus_tag="KRL67_002477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBH1523347.1"
                     /translation="MEEKYILSDRGKVYYWIKESNSVFAKNLIFLPGLTANHHLFDFQ
                     IQYFSKNHNVLVWDAPAHGKSRPYLDFSYSNLVEELKVILDKQNMKKVVLIGQSAGGF
                     VAQSFIVKYPNMVEGLMMIGTCPYGIDYYSKSDIFWLKQTEWMLKLFPDKLLRNIISK
                     MCGATSEGRRSMMKMLEGYEKKELCHLMYLGFAGFIPEIQDLNIHCPVCLVVGDKDKT
                     GKVRKYNEQWHNKTGYPLHIIKNASHNVNVDKPNELNQIMDIFIKQLNI"
     gene            8107..8997
                     /locus_tag="KRL67_002478"
     CDS             8107..8997
                     /locus_tag="KRL67_002478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HBH1523348.1"
                     /translation="MIESIPAKQILQKVKFDNTRWFGIDYNMNLYRGCSHGCIYCDSR
                     STIYNIENFDKVRYKENVIEILSKELRSKRKKGVVGIGAMSDTYNPFEKQLCITKQAL
                     DLISENHFGVSIDTKSSLVVRDIPILKKIKKNNSAIVKLTITTANDELSKKIEPYVNP
                     SSVRFDAVKELNDSGIFCGILLTPMLPFLTDTKDEIRAIVEKAHKANAKFIYCMYGVT
                     MRSGQREFFYEHLRGISPKLVFKYQKTYGLNYVCTIQNKDSCEKLLREECARYGILTD
                     MKDIIKAYKMSESYTQIKFF"
     gene            9311..9754
                     /locus_tag="KRL67_002479"
     CDS             9311..9754
                     /locus_tag="KRL67_002479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBH1523349.1"
                     /translation="MIEIKTIEINKKGQIDLLMLADPCEEMIDKYLNKGTMYALYDNE
                     ELTCIAVVNEISKEICELKNIATYEHFQNMGYASKMIYYLLDVYSKKYSSMIVGTAES
                     GVSFYEKFGFVYSHKIKNFFVDNYPEPIFEGELQCVDMLYLSYNF"
     gene            9926..10096
                     /locus_tag="KRL67_002480"
     CDS             9926..10096
                     /locus_tag="KRL67_002480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH1523350.1"
                     /translation="MDSNNNTIKSTVKKGISFGSCLAMIISYTAWKSIPWAIFHGLMS
                     WIYVLYYWVKYA"
     gene            10469..11566
                     /locus_tag="KRL67_002481"
     CDS             10469..11566
                     /locus_tag="KRL67_002481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HBH1523351.1"
                     /translation="MKEKLEEKSKIIKTSKEYKRTVIEEKDREWTKKLNDILSRGDFS
                     NEKLDKIKDLIPKEILTSENVGEVVTEDGYAKPEYDEVFKSLFTLNNDYDLLANFIND
                     ILKDAPYANRNIKPFTQIKRIIKVETDPTINYIGEKRPRLDILAEDEERNHINIEMQR
                     ALENDYLERAEYYLSRVHGRKLEEGKEYKEIGKTVGIHILNHVKYNHIEDYVNCLRLT
                     MDGHPDIFSSKTALYFIELPKIRKSSCIANRVLIWGKLIDNPSHIDIRILSKTDYVIK
                     RALDRLKELGSNEDYLLNLKRGAYIMNKSRNFKEEIFQEGIEKGIAKGKREEKFNIII
                     KLKNKGYNLSEICDIIDDLNKSEVEKVYNQN"
     gene            11771..12259
                     /locus_tag="KRL67_002482"
     CDS             11771..12259
                     /locus_tag="KRL67_002482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3841 domain-containing protein"
                     /protein_id="HBH1523352.1"
                     /translation="MKFYTIQTLEFWNNNKNNKYLSNDYSLIVEDFDIPYKWMYSQMI
                     ERIPKFDDSMIWVWSKRPDLRFSAYLERGLKGVLLEIEISEDNVLLSEFDLWHLPLMD
                     IAVEIYEDEQIEKQVSWERIFDLELCSKLNEVNIEDLTLQGVTSKVPLSNVRLIKEFV
                     AK"
     gene            12345..12752
                     /locus_tag="KRL67_002483"
     CDS             12345..12752
                     /locus_tag="KRL67_002483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH1523353.1"
                     /translation="MSKQQTGIKSTLISEILGISYCIGATLVNKYFFKGSSIEYLPVI
                     IWVLAMGAQWVYNKKYDDLIDEASKCILSRVNDIAIKVLFFSVAIVSIFLVTLDVNVS
                     NLNIGMSLLVILLIQSLLKLILFIYFDRKGIYN"
     gene            12753..12971
                     /locus_tag="KRL67_002484"
     CDS             12753..12971
                     /locus_tag="KRL67_002484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBH1523354.1"
                     /translation="MSVLLKTKIKSYREKNLMTQGELAKSVGVRRETIVHLENGRYNP
                     SLKLAMDIAKVFDTTVEELFEFVEENNI"
BASE COUNT         5282 a         1501 c         2331 g         4475 t
ORIGIN      
        1 ttcaatatga gattaacttg tgattaatag agtaaggtgt aactaaagat aaacttcaat
       61 ataagtttaa cctgtaacta atagaataag attgtgaata aagttatagt tttagatggg
      121 tattaacctg tgactaatag aatatgacta ggtgtaaaac tatagagttg tataagaaaa
      181 tattatagat atattaaata ttagataata aatatttata tagatacaca gaagaaaagg
      241 cgatatgaag ttaaacttaa tgacttcata tcgtttttta tttgaaattc ctttgtattt
      301 agagtgtgac ttttaatatt gaggttttat gtgattaagt cacagataaa aagtaagtat
      361 cttgatataa ttattataga ttaataatat agggaggtta ttatgtctaa taaaaaaaag
      421 aagtatgcaa atattttagc aaatgagtgc gttgcttgtg gtagctgtat aaaagcatgt
      481 ccaagaagtg ctatctcagt tccatgtggc attagtgcaa aaattaatag agatttatgt
      541 gttggatgtg ggatttgtga aaagatatgt ccagcatctg taattgagat tataacaata
      601 ttaaaagagg aagaaggtaa gtgtcatgaa taataaaaaa cattggtatg actatttatg
      661 gattttttct tcaatatatt tgatattggg atttgtaaat attattttcg cttggattgg
      721 gctaatttgt ttctttgttc cactagctat ttctattgtt aagggaaata aagcttactg
      781 taataagtat tgtggaagag ggcaactgtt taatttgctt ggaaataaat tgaagctgtc
      841 aagaaataga gatattccta aatttattag aagtaagtgg tttagatatg ggtttttgac
      901 attttttatg gtaatgtttg ctaatatgct tttttcaact tatttagtat ttgaaggaag
      961 tagaaatttt aaagaagtag tgactttgct gtggactata aaaatgcctt ggcaatgggc
     1021 gtatcatgga actttagttt caccttgggt ggtacagttt gcatttggtt tttatagtgt
     1081 tatgcttaca tcaactgttc ttggtttgat tactatgatg ttgtttaagc ctcgttcatg
     1141 gtgtgtttat tgccctatgg gtactatgac tcagatgata tgtaaggcaa agagtaagtc
     1201 tgtatagaaa ggctttatgt gatgtttaca gtgttgttca agtagagata agtagtaggt
     1261 ttgaatatat gtgacaaata tatgagtata ggggcataga tttagtttgt gtaaggtaaa
     1321 ctgatttatg cttctttttt atgtcttaga ttattggcta aatggaaaca atgaatttag
     1381 atattaagaa ttattcagtt atttgcaaaa aatataataa taattaacat tatatataag
     1441 ccaattaata gcaagattta tttgatataa aataggtcaa gcatttaaac taaataaggt
     1501 cataacgatt aatataagta tatatataaa aagattagaa gttataactt ataaaagata
     1561 ttctatataa atagtatggg aaaaatagtc aaactaactt attagaaaca aaaaatatac
     1621 ataaaaatga atgtaaatat caaataaggt acgatatcaa aatagatata agcatctttt
     1681 ttattattga aattatgaaa atacatttga tttcacacaa aaaagagtgc ctcatgaact
     1741 aaaaagttca ttttgagaca cacttttaca gtgtcaaaga acgaagttta tccttatcca
     1801 atacttttat accaccacga gataaagaca caataccttc tctagcaaaa tatttcaaca
     1861 ttctcgatac aacttctctg gcactaccca tgtacttagc aatttgctca tgagtcatat
     1921 ttatagtgct actgccattt ttagctatct catcaattaa aaaagatgcc aaacgcttat
     1981 caaaactcat aaaaagaatt tgttgcatag cccacataac atcagaaaaa cgttcggtag
     2041 ctaatttata tgaaaataac tctgcatgaa tattttctgt agataatttt gcaaaaacag
     2101 aggaactaat ctgtataata tcgcagtccg tttcagcatc tacatagaca tcaaaagtta
     2161 ttgtttttag aatacaggaa gctgataaaa tacatacatc tccatcatca agtcggaaga
     2221 gtgtaacctc tcgaccttca tctgaaagta tgtatgtacg tactgttcca gactttataa
     2281 tcaaaatacc aatacagtca ctatcaccac aatgtactgg attccctttt ttgtaatgtg
     2341 aggtatttgc tgaagaaatt aataattctt tttgtaagtc agtaagttta tcccaaaaag
     2401 gcaagctatg tgtaagtata tcaatttcat tttgtgaaat catacaatat ctcctttctt
     2461 aaattgttat aatcccatga tacaagtgaa tagataaatt gacaagtagt ttgttaagag
     2521 gattatttag tcagatagta atagtatgat ttatgtagaa aaaatatgat aatatatatt
     2581 cttgctatta atgagaaagt gatatatttt gatgatagta ggtttgtata actagtgcat
     2641 acagtttatt tttaaaagat taagttcctt atgtagcctt atagacataa aaccagcaat
     2701 tatagcagca atcaaatcgg ctattggttg tgcatataag ataccattta acccatagaa
     2761 tacaggaata atcaaaatta tagggataaa acagatacct tgtctacaca tacctaaaaa
     2821 tccaccttct tttgcttttc ctagtgcgag gaataatgta gaatacactg tatagaatcc
     2881 aaatagcata aatgaaaaac catttgccct aagagcttta ctaccatttt ctattaatag
     2941 aatgtcacca cttgtaaatt gtgaaattat agattttggg aaaagcacca atataagacc
     3001 aagcattaca caaaatattg tagtccataa tatggaggtt tttattgcta tatgtaatct
     3061 atcgtagttc tttgaaccaa aattataacc agcaattggt tggaaacctt ttataaaacc
     3121 aaatacaata aggctaccta aagatgttat tcgtgttaca gcacccattc cagctatcac
     3181 agaatcacca tactttgtag cctccatatt agttaatgta atggcaagac tagtcaataa
     3241 ttgaaatatt aaggtaggta tgccaatttt taggatttct gaaaaaattt cttttgaaaa
     3301 acaacattca ctaattctaa aactaaatat acttttcttg cttaaaatat atcctatata
     3361 aacaagtgtc gaagcaatct gtgagatagc tgttgcaata gaagcacctc ttacacccat
     3421 accaaaagta taaatgaata tcgggtctaa aatcatatta agcaaagcac ctaataacat
     3481 agcacacata gttgttttag cagctccttc acttgtgaca atattagtca tagtgacgtt
     3541 gaatatatta aaaatagatg atactacata aatgctagca taagatattg cataaggcag
     3601 gatacttttt gtagctccta acaattttaa aattggattg agaaaaatca taatcacaat
     3661 aataactaat gtaccaacta taatactact gtaaatagat gtactgacca ctttccctgc
     3721 tttttcatta tcaccatgac ctaaaagtct ggcaatgtat gaagcagctc catttccaaa
     3781 caataatcct aatcccacaa caagctgacc taatggaaat gcaacggata tagctcccat
     3841 ttgacttgtt ccaagtccac ccacaaagta tgcatcaact aaattgtata atgcagttat
     3901 catcatacct atcatggtag gtaatcccat agataacaat gctttcgaga ttggggcaga
     3961 ccctaatagt tcaattcttt tgttactttc tttcatagtt aacactccta ttctattgac
     4021 attaagtcta aattataata ctataaatta tagtaagtat ggaaaatagt actataattt
     4081 atagtatttg tcaatagtaa gaaaactaaa ggagatgatt tttatggcta caatagattt
     4141 aattgtattg ggaatagtaa agcaacaacc taagagtgct tatgatattc aaaaggcagt
     4201 ggaatacagg aatatatcac gttgggttaa aataagtaca ccatcaattt ataaaaaagt
     4261 aattcaatta gaacaaaagg gatatattga aagtaaaacg gtgaagaatg gaaaaatgcc
     4321 agaaaaagca gtttattcat taactgaatt aggtgagaca cattttttag atttaatgtt
     4381 tgatacatct agtagaccaa taaatattct tttagatttt aatgctgtta tagtaaattt
     4441 aaatagcctt acaccagaaa aacagaaatt atgtctcaat aatattgagg agaatgttca
     4501 agagttaaag aaaactattg aggaaaatat aagtataaag acagaagtac cagagacagc
     4561 aaaagcagtg ttggagcaac agttggtgtt agcacaagca ttggaaactt ggataaattc
     4621 tttaaaagat aatatgggaa attagaatta gagaagctaa cttaataatc attaaacaaa
     4681 aaacagcata tttaataatc agatattaga aaaaatgaaa caaatgatat aaactgaaaa
     4741 gtatatttaa aataaagctc acttaattga gagggaatgt taaaattttt aagaggaaga
     4801 gcaagagcaa tcagtaaagt ataaaattct attactataa taaaagatag aactagaaga
     4861 ctttatgaaa gtgagaaaag tatttatata aataaattta tagagtctat agtttaatag
     4921 taggaaatat cgtattttac ataaagattt agcaatatga cacactgttg tcgtgttctg
     4981 taggatatga ttaataaatc agatataaaa ggagagtgag taatatgcca gaattgctaa
     5041 aaaacatgta taatcgtgag tcactttatg aagttgcagt agctatccaa tcagtttaca
     5101 actcatttaa ggtagacgag ttcataaaat atacaatgga tgagacatgg aataacttag
     5161 aattaaaagc cagatgtcga aagataagta tgagcttagg aatgtattta ccagaagact
     5221 ataaggaagc tctttccatt cttgaaaaat cagttgcagg tttttatttt gcttttttct
     5281 ttccagattt tgttgaggtt tatgggcagg acgacgtaaa ttgggattta tctataagtg
     5341 cattagaaag aaatactgag tattggtctt cggaatttgc tgtaagagca tttatcatca
     5401 aggatgaaga gcgtatgatg gcacagatgc gtaaatggtc taagcataaa aatgaacatg
     5461 tacgaagact tgcaagtgaa ggctgtcgtc cccagttgcc atggggacaa gcaatagcca
     5521 aatttaaaaa agaccctaca cctgtactgc ctatattgga gcaacttaaa acagatacat
     5581 caacgtatgt acaaaagagt gttgctaata atctaaatga tatctcaaaa acacatccag
     5641 atttagttgt aagcattgca aaggactggt acggtaaaaa taaatctact aactggattg
     5701 taaagcatgg ttgtagaact ctgctaaaaa aaggaaatcg tgatgtatta gctctttttg
     5761 gatatgatga tgctacttca ataaatattc aggactttac tttagaaaca acgtctattt
     5821 ctattggaga ggatttaact ttttcattta atattttagc aaaaaaagcg acaaaaacaa
     5881 ggctcgagta cggaattgat tatgtgaaat ttaatggaaa aaggaatagg aagatattta
     5941 aaatatcgga ggtttcatta aaagaaaatg agaaaaagtc atatatgaaa aaacattcat
     6001 ttgcagatgg aagtgtaagg agacattatc caggcatcca ctcgattact attattatta
     6061 atggggtaga aaaaggtaaa cttgattttg agttaggtgt ataattttac taacagcata
     6121 acctatatca gatactttgc taaacctata tcatatattc tactaaatct acattaggta
     6181 gtttactact tgtttactga atttaaatac aaagagacac aggtgctaat ccctgccatt
     6241 aaaggtatat gagcataaca caaaagtaga acttatcaat tctgttacag gtacagtagt
     6301 aaatcctcag aatcacaatt agcaaatgaa gtgtaaaaaa taagtgactg tttattttgg
     6361 ttgatagtga tattggatag ttatacaata ccaatataaa ttagaatttg tagaggagaa
     6421 atataaatga atgtatatag tttttggaat gacactttgg cacaagatgc tgttaagata
     6481 agaaaatatt ttcatgaaga tgcttatatt aattggcatg atacaaatga gcaatttact
     6541 ttagaagaat ttattattgc aaattgtgag tatcctgata aatggaatgg taaagtagaa
     6601 agggtgctag aatttggaaa tttagtaatt acagtgacac atgtttatgc aataaataaa
     6661 ccactatcat ttcatgtcgt ttcatttatc agccttaaag atgataagat tatttctatt
     6721 gatgaatatt ggggagagga tgatgatgtg ccacagtgga gattagataa acatattgga
     6781 aagccaataa aatagcgttt gttctgtttt ataactgaaa ttttgataac atttgatatt
     6841 ttgatttgtc attggtcagc atagtcgtta gtgtaataat ttaaaaaagt agaatttgga
     6901 gagtgaacac tatggaagaa aaatacatct tatctgaccg agggaaagta tattattgga
     6961 taaaagaaag taatagtgtg tttgcaaaga acttaatttt tttgccagga ctaacagcaa
     7021 atcatcatct ttttgatttc caaatacagt acttttcaaa aaatcataat gtattggtat
     7081 gggacgctcc tgctcatgga aaatcaagac cttacttaga tttctcatat tctaacttgg
     7141 tagaagaatt gaaagtcatt ttagataaac aaaatatgaa aaaggttgta cttatagggc
     7201 aatcggcagg gggatttgtg gcacagtcct ttattgtaaa atatccaaat atggtagaag
     7261 gactaatgat gattggaact tgtccatatg gaattgacta ttatagtaag tcagatattt
     7321 tctggttaaa acagactgaa tggatgttaa agttattccc agataaattg ttgcgaaaca
     7381 taatatctaa gatgtgtgga gctacgtctg aaggcagaag gagtatgatg aaaatgttag
     7441 aaggctacga aaaaaaagaa ttgtgtcatc tcatgtatct gggatttgct gggttcatac
     7501 cagaaattca agacttaaat atacattgtc cagtttgttt ggtggttgga gataaagaca
     7561 agacgggtaa agtgagaaaa tataatgagc agtggcacaa taagactggg tatccattgc
     7621 acattataaa aaatgcttca cataatgtaa atgtcgataa acctaatgaa ctaaatcaga
     7681 ttatggatat atttattaag caattaaata tataaaaact acatactcat taggtaaatt
     7741 atgtagtata tttgtttaca gatagttata gaaataaata tgtaataaac tacttaacac
     7801 ttaaaaagtg gatttgtcca gagcataata caaagcatga tatatataaa tttttaagcg
     7861 aaatacatca aaagaaggat tagaattagt ggagacagaa cagctattag agattataaa
     7921 tagttttaaa ggaggggtac tacgaatata gaggaaggta aatcatacgt tattcaagga
     7981 ttataaatga ttagtttaaa agtactatcg gataggactc tgcatattta tttaaacctt
     8041 agtaaaggta gttaaagtag tagtttaaaa actaaaaaat tatataaagg aattaggtat
     8101 aagaaaatga ttgagagtat accagcaaaa caaattttgc aaaaagtgaa atttgacaat
     8161 acaagatggt ttggaattga ctataatatg aatctctatc gtggttgttc tcatggatgt
     8221 atatattgtg atagccgtag cacaatatac aacatagaga attttgataa agttagatat
     8281 aaggaaaatg tgatagaaat tttaagtaaa gaattgcgtt caaaaagaaa aaaaggggta
     8341 gtaggcattg gagctatgtc tgacacatat aatccttttg aaaagcaact ttgtattaca
     8401 aagcaggcac tggatttgat ttcggaaaat cattttggag tgtctattga taccaaaagt
     8461 agtttggtgg ttagagatat accgattttg aagaaaatta aaaaaaataa ctctgctatt
     8521 gtaaagttga ctataaccac tgcaaatgat gaattatcaa agaaaataga accttatgta
     8581 aatccaagtt ctgtacgatt tgacgcagtg aaagaactta atgattctgg aattttttgt
     8641 ggcatattat taactcctat gcttccattt ttgactgata ctaaagatga aatacgagca
     8701 attgtagaaa aagcacacaa ggctaatgca aaatttattt attgtatgta tggtgtaaca
     8761 atgcgtagtg gtcagcgaga gtttttttat gagcatttaa gaggtatatc accaaaattg
     8821 gtatttaaat atcagaaaac atatggttta aactatgtat gtactataca aaataaagat
     8881 tcttgtgaga agttgttaag agaagaatgt gcacgttatg gtattttgac ggatatgaaa
     8941 gatattataa aagcatacaa gatgtcggag tcatatactc aaataaaatt tttttaagat
     9001 aggctttgga taagatataa tagtggtgat tgatgtgttg atattatagt tgattgctag
     9061 agctaatatt atagatgaat attaatattg atactctagg taattgctag agctaatgtt
     9121 atagatgaat attagtattg atattctagg taattgctag tgctgatatt ataatttatt
     9181 attagtatta atattctagg gaattatcac tatattttaa aaaagtaaaa atatatatca
     9241 aaatgaatta aataagtact gtaagaagtt tgctaaacta aggtttgaat aaagtgaggg
     9301 tggagaactt atgatagaaa taaaaactat agaaataaat aaaaaaggtc aaattgacct
     9361 tttaatgtta gccgacccat gtgaagaaat gattgataaa tatcttaata aaggaactat
     9421 gtacgcattg tatgataatg aagaacttac ttgtatagca gttgtaaatg aaatttccaa
     9481 agaaatatgt gaattaaaaa acattgctac atatgagcat tttcaaaata tgggttatgc
     9541 ctcaaagatg atttattatc ttttagatgt ttatagtaaa aaatattcat caatgattgt
     9601 tggtacagct gaaagtggag tttcctttta tgagaagttt ggatttgttt attcacataa
     9661 aattaaaaac ttttttgtag ataattatcc agaaccaatc tttgaaggag aattgcaatg
     9721 tgttgacatg ttgtatttat catataattt ttagatagga taatattttt aggtagatat
     9781 aattaattta ttcatataat tattggaatt ataatttata aattatgtat aataagagta
     9841 taacttattt agaagctgtt atattgtatt agattcaaat aagatatata ataacttgtt
     9901 tattttgaat tttggaggag taattatgga ttcaaataat aatactataa aatcaactgt
     9961 taaaaaaggt atttcttttg gttcttgttt agcaatgatt atttcttata ctgcatggaa
    10021 atctatccca tgggctattt ttcatggctt aatgagttgg atatatgtac tttattattg
    10081 ggttaaatat gcatagatta aaataataaa taagtatgta atattgtgaa gtttaagaaa
    10141 ccaaatgtaa gggagtgtct cgaagtgaac tttttgttta taaggcactc ttttttgtgt
    10201 taaataaact acattttcat tactgaaaca atgaaaaaga tgattatctc tattttaata
    10261 cataccctta tattcattca tttatcaaca atagttttaa aaaaaaagtt atctaaaatc
    10321 tattgtaatc taattgcttc tctctaaagt cttaacttta gtaatgatat atgagtttat
    10381 caggttctta tttcaataat gcaaattttg cttagaagtt cagactttgg tatgaagaaa
    10441 tgggtaataa taaaaaagag gtgttgatat gaaggaaaaa ttagaagaaa aatctaaaat
    10501 tattaaaaca tcaaaagagt ataagagaac tgtaattgaa gaaaaggaca gagaatggac
    10561 caaaaaatta aatgacatac ttagtagagg tgatttttct aatgaaaaat tagataaaat
    10621 caaggatttg attccaaaag aaatattgac tagtgaaaat gttggagaag ttgtgactga
    10681 agatgggtat gctaagcccg agtatgatga ggtatttaaa tcattgttta ctttaaataa
    10741 tgattatgat ttacttgcaa attttataaa tgatattctc aaggatgctc catatgctaa
    10801 taggaatatc aaaccgttca ctcaaataaa aagaataatt aaggttgaaa ccgacccaac
    10861 aattaattac attggtgaga aaagacctag acttgatatt ttggctgaag atgaagaacg
    10921 taaccatata aatattgaga tgcaaagagc tcttgagaac gattatttgg aaagagctga
    10981 atattatctt agtcgtgtac atggtagaaa gttagaagaa ggtaaagaat ataaagaaat
    11041 tggaaaaact gttggaatac atatactaaa tcatgtaaaa tataatcaca tagaagatta
    11101 tgtaaattgc ctgagattaa caatggatgg gcatccagat attttttcta gtaaaactgc
    11161 attatatttt atagaacttc caaaaattcg taaatccagt tgtatagcaa acagagttct
    11221 aatatggggt aaacttattg ataatccttc tcatatcgat attagaatac tatctaaaac
    11281 ggattatgtt attaaaagag cacttgatag attaaaagaa ttaggcagta atgaggacta
    11341 tttattaaat ttaaagagag gtgcgtatat tatgaataaa agtagaaatt ttaaagaaga
    11401 aatttttcaa gaaggtattg aaaaaggtat tgcaaaaggg aaaagagaag agaaatttaa
    11461 tatcatcata aaattaaaaa ataaaggata caatttgagt gaaatatgcg atattataga
    11521 tgatttgaat aaatcagagg ttgaaaaagt ctataatcaa aactaatact atgattaaga
    11581 tagcactaga taaatacaaa gcttcctctt ttaaatttaa attaatataa aactttgtta
    11641 tttaaatatg gtagatataa agagatatat aaataatagg tacaaagagg tgtataaata
    11701 aaagagataa ttcaaaataa aaatttgaat tatcttcttt ttatagaaaa agaaaggggt
    11761 tgatttatat atgaaatttt atactataca gactttagaa ttttggaata acaataaaaa
    11821 caataaatat ctatcaaatg attattctct tatagtagaa gattttgata ttccatataa
    11881 atggatgtat agtcaaatga tagaaagaat acctaaattt gatgatagta tgatatgggt
    11941 ttggtcgaag agacctgatt taaggtttag tgcatactta gagaggggtt taaaaggtgt
    12001 tttacttgaa atagaaataa gtgaagataa tgtgttatta tcagaatttg atttatggca
    12061 tttaccttta atggatattg ctgtagagat ttatgaagat gaacaaattg aaaagcaggt
    12121 aagttgggaa agaatatttg acttagaatt atgctcaaaa ttaaatgaag tgaatataga
    12181 agatttgaca ttgcaaggag tgacatctaa agtacctcta tctaatgtca gattaataaa
    12241 agaatttgtt gcaaaataat atttttattg aaatttattc aaaaaagtta tatatttata
    12301 acaagaggtg ttataaatat ataacttttt aagggggatt ttatatgtct aaacaacaaa
    12361 ctggtattaa atcaacactt atttcagaaa tattgggaat ttcttattgt attggagcta
    12421 cactagtaaa taagtatttt tttaaaggaa gtagtattga atatcttcct gtaattattt
    12481 gggtattagc tatgggagct caatgggtct ataataaaaa gtatgatgat ttaattgatg
    12541 aggcatcaaa atgtatacta tcaagagtta atgatattgc aataaaagta ttgttttttt
    12601 ctgtagctat agtatctata tttcttgtaa ctttggacgt aaatgtgtca aatttaaata
    12661 taggaatgtc tttattagtc attttgctta ttcagtcctt attgaagtta attctattta
    12721 tatattttga cagaaagggg atatacaatt aggtgtcagt attactaaag acaaaaatta
    12781 aatcatatag agaaaagaac cttatgacac aaggtgaact tgcaaagtca gtaggagtaa
    12841 ggcgtgaaac tattgtacat ttagaaaatg gtcgttacaa tccttcttta aaattggcaa
    12901 tggatattgc taaggtattt gatacaactg tagaagagtt gtttgaattt gttgaagaaa
    12961 ataatattta aatttcatgt attatttatg tattagagag tgaattaata gataaaaaat
    13021 tcaagtttat gtattagaga atgaattaat tataggcaaa aaaacaagct tatagataag
    13081 ataataaatg atttgtagat gaaaaaacaa gctaatggat taaagaataa atgatttata
    13141 gatgaagaag aaagcttata gattaaagaa tcaatgattt ataggtaaaa accaagctta
    13201 tagattaaaa aatgaatgat ttatagatga agaactaagc ttatagataa gagaataaat
    13261 gacttatagg taaaaaccaa gcttatagat taaagaatga atgatttata gatgagaaac
    13321 caagcttata gattaaagaa taaatgactt atagataaaa aaccaagctc atagattaaa
    13381 gaataaataa tttatagatg aaaaaccaag cttatagata agaagtccat gcttataagc
    13441 cagaaaatga atagtttaat agattaaaaa atacatattt gtgaattata gaatgaataa
    13501 ctccataaac tagaaaattt ataataatat ctatatgaaa attagaagta ttttatcaca
    13561 aaaaattaag ttaaataaat atgaaatta
//
LOCUS       DAEPXF010000099        13321 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain HB27
            SAMN13376389-rid12479423.denovo.105, whole genome shotgun sequence.
ACCESSION   DAEPXF010000099 DAEPXF010000000
VERSION     DAEPXF010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13376389
            Sequence Read Archive: SRR10518432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 13321)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13321)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001069624.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001069624.1
            Genome Coverage       :: 315x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/15/2021 01:54:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,165
            CDSs (total)                      :: 4,076
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 89
            rRNAs                             :: 10, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 10 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 19 of 69
            Pseudo Genes (incomplete)         :: 44 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 10 of 69
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13321
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13376389-rid12479423.denovo.105"
                     /strain="HB27"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="China: Hebei"
                     /collection_date="2017"
     gene            125..1201
                     /locus_tag="KRL67_002485"
     CDS             125..1201
                     /locus_tag="KRL67_002485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HBH1523355.1"
                     /translation="MKAQRLNAVLEQMKKDDISQMLVSDPTSIFYLTGVLIHPGERLL
                     ALYLNLNGNNKLFINELFPVSEDLGVEMVWFNDTQNPVEIITEHIDKNATMGVDKNWP
                     ARFLLNLIELGGGSKFVNSSYIIDTLRMCKDEEEKELMRIASKLNDKAMEQLKATVSG
                     ELTEKQLVGKLSKIYEDLGTDGFSFDPIIGFGPNGANPHGEPGNALVKPGDAIILDIG
                     CIKDNYCADMTRTVFYKEIPEKGREIFEIVLEANKRAEAIVKPGVRFCDIDAAARDYI
                     TEKGYGQYFTHRTGHSIGLEVHDKGDVSSINTDTVQPGMIFSIEPGIYLPGEFGVRIE
                     DLVLVTEDGCEILNKHDKEICVVG"
     gene            complement(1304..2146)
                     /locus_tag="KRL67_002486"
     CDS             complement(1304..2146)
                     /locus_tag="KRL67_002486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HBH1523356.1"
                     /translation="MDKIKLIVDSACDLPDDIIEKYNIEVVGLNVSFGEESYISGKEI
                     DNETFYRKMSESKVLPKTSCPSPDKFLESYHCQEESVLVINISSGLSGTYNSALLAKD
                     MFEKEGNNKKVEVIDSLSGSIGIGQLVLKAAILIKEGKEIEEIVSIINKYKDNPPFFG
                     TLETLENAIKGGRINPIAGKIINTLNFKAIIQVADGVVTPIDKARGEGNSLKKLITLV
                     ESKIKDKEEKVLFIGHANCPEKAQKVREVMEKDVKYKDVVICEVGSVMGTYTSKGAIL
                     ITAI"
     gene            2330..2902
                     /locus_tag="KRL67_002487"
     CDS             2330..2902
                     /locus_tag="KRL67_002487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBH1523357.1"
                     /translation="MKTNNMKQAILSSATNLIAKNGVQNTSLADIAKDVNISKGTLYY
                     HYASKNDIIYDIADTHLEVITSAILNCVKNVKSKNSQIEMVNLILEKISTIESRGRVH
                     MYLICEAITSNNDLKERMRLKYIEWRTTLKSEIVESLEKYNSSENEQDAESFSFLLMS
                     IVDGLVVQSLLKTEKIPYENIASFLVNHWI"
     gene            complement(3065..3337)
                     /locus_tag="KRL67_002488"
     CDS             complement(3065..3337)
                     /locus_tag="KRL67_002488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH1523358.1"
                     /translation="MLRERFVVRNHHNPHTSGCCGTGGEGCCGGDNHEHSHQHSHSHG
                     HEHEHVNGVGHDHNHDDGFSGTHEHSHEHTHTHGHEHSDGHTHEHK"
     gene            complement(3451..4323)
                     /locus_tag="KRL67_002489"
     CDS             complement(3451..4323)
                     /locus_tag="KRL67_002489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HBH1523359.1"
                     /translation="MYGESKKLSIDNNIFLQYCLIILGMILSTLGINLYLAPAKLLSG
                     GVAGICVILYKLFGINQGISSFLMNIPIFIIARKYFDNKFLLISFVNMLLFSLALGLT
                     QDIAKYFPIDDTMLQCIYGGALTGIGMGLTFKARATAGGLDIIAAIMKRKYDIPMKNT
                     FLFINFFVVCAGAFLFGAKLVMYTLITMYIISFTMDIGKDCFDRKKSILLISNKYDEI
                     SKVIMNKMGRGVTFLEAEGAYTQNKKKMIYCIISANEIGKFKDLIYDMDSEAFISVNN
                     VEEVKGGGFKDKFL"
     gene            complement(4619..7726)
                     /locus_tag="KRL67_002490"
     CDS             complement(4619..7726)
                     /locus_tag="KRL67_002490"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HBH1523360.1"
                     /translation="MAKFKPLVDSSVKQAEAQVFDYWKDINILEKTLEKGKDDPSFVF
                     YEGPPTANGNPGVHHVLSRTLKDSVCRYKTMSGYQVKRKAGWDTHGLPVEIQVEKELG
                     LTSKQQIEEYGIAEFNQKCRESVFSFEKQWRIMTERMAYEVDLDNPYITLDNNYIESV
                     WWILNKFNKEGYIYEGHKILPYCPRCGTGLASHEVAQGYKEVKTNTVIAKFKKKDADE
                     YFLAWTTTPWTLPSNVSLTVNADVDYLKVKKGDEVYYVSKPLANKVLGEDYEVLEEMK
                     GKDLEGLEYEQLMPFVEVDKKAFFITVGDYVTTEDGTGIVHTAPAFGEDDYNTGKRYD
                     LPVIQPVDETGKFTSTPWEGRFVMEDGLDVEIIKWLASENKLYSKEKVVHNYPHCWRC
                     QTPLVYYAKPSWYIEMTKLKDKLIENNNGVKWFPSFVGEKRFGNWLENLNDWAISRTR
                     YWGTPLPIWRCECGHTDSVGSRAELAEKAIEDVNPETVELHRPYVDDIHLKCEKCGKP
                     MTRVTEVIDCWFDSGAMPFAQHHYPFENKENFDQLFPADYISEGIDQTRGWFYSLLAV
                     STFVLGKAPYKSVLVPDLVLDKDGKKMSKSRGNTVNPMELFDQYGADALRWYLLYVSP
                     PWTPTKFDMDGLKEIQSKFIGTMKNVYNFFTLYANTDDINPTEFFVEYKDRPELDRWI
                     LSKFNNLMKDVEENLAIFELNKTLRMIQDFINDDLSNWYIRRSRRRFWATELTEDKKA
                     VYNTTYELLHGLCRAIAPFAPYMSEEMYRNLTGEVSVHLAEYPKCNEELVDTKLEEKM
                     DLAKNLVTLGRASREVERIKVRQPLQKVLVDGKFEDTISDVVDLIKEELNVKEVIFAK
                     DLDEYMNFSLKPNFKEAGPLLGSKMNLFVGALSKLNAHETANKLEKGETLTVDLDGEA
                     FEFNKDLVLIGITAKEGFNVSVENNLFVILDTKLTEELINEGYAREFISKVQQLRKSN
                     NFEVLDNIVIEYCGDDEIAKAVDHFDEYIKSETLALEINRVDDKSLEEQNLNDHMTGI
                     KVIKK"
     misc_binding    complement(7818..8050)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(8225..8752)
                     /locus_tag="KRL67_002491"
     CDS             complement(8225..8752)
                     /locus_tag="KRL67_002491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HBH1523361.1"
                     /translation="MLTPIEIENKEFKKGLRGYRDEEVDEFLDIIKEDYESLCRENTA
                     LKEKLGLYQDQVNKYENIEETLKATLITAQSAAEDTCSAANKKAKIIVEEADLKARQI
                     IEQANNRVIEIRKEYDALVKEFKIFRNKFKSLLEDEIRSVDEIFYDVDEKCVNAFEGT
                     TVYNYSNDEAATTLE"
     gene            complement(8787..9572)
                     /locus_tag="KRL67_002492"
     CDS             complement(8787..9572)
                     /locus_tag="KRL67_002492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HBH1523362.1"
                     /translation="MDKLKLTNHIKDIDLKNKMFKVIDKANSCIKNYDVKSTEFLNPY
                     EVKNAVAILNSTNEIKYSVDGGYEQAERSTVFIYPFYMEYEDIEDTLRFLQIEGNFKF
                     KNISHKDYLGSILSLGIKREKIGDIIIHDSFCQVVVSSDICDFIIVNLEKVSRNNVIV
                     KEISRENIVNSSSKYKEVSFTVSSDRLDCVISGIYNISRQDSAKYINGEKVHVNYEKI
                     TSTSKIVKNDDLISIRGKGRAKVTQIGDITKKGKIKVQARLIV"
     gene            complement(9582..9839)
                     /locus_tag="KRL67_002493"
     CDS             complement(9582..9839)
                     /locus_tag="KRL67_002493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HBH1523363.1"
                     /translation="MGTIRIALYYLLDIIAWMIVIKSLMTWFPNGRQSKIFDILENLT
                     EPIEGPIRSIMYKYTNGPIDFSPLIAIVLLMFLRQLVLVIF"
     gene            complement(9858..10316)
                     /locus_tag="KRL67_002494"
     CDS             complement(9858..10316)
                     /locus_tag="KRL67_002494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HBH1523364.1"
                     /translation="MSNGIISKFKNWIVDEEEDYIEDEYESGMDDIVQEEEMNSGFST
                     AKANKIVNLHTTSQMKVVIVEPKVYDEAATIADHLKQRRAVIVNLEGLTNSEVRKSIF
                     NFMNGAVYVLDGSIQKVSKSIFILAPNNVDIDANMKKELESKAFFPWQNK"
     gene            complement(10356..11060)
                     /locus_tag="KRL67_002495"
     CDS             complement(10356..11060)
                     /locus_tag="KRL67_002495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HBH1523365.1"
                     /translation="MGSIKDNLSEIRNKIDKAAKKVDRDSKEITLLAVTKTVDVDLVN
                     EAMREGITSVGENKPQELARKYDIIGDKLNWHLIGTLQTNKVKYIIDKVCMIHSLDRI
                     ALCEEIQKRAEQINREIDCLVQVNISKEESKHGLDREEVVDFVKEVSEKYKNIKIKGL
                     MTMAPFIEDEDEIRGVFKGLKDLSKQIKELNIPNVGMDVLSMGMSHDYEIAIEEGATI
                     VRVGTSIFGERNYNKK"
     gene            complement(11070..11705)
                     /locus_tag="KRL67_002496"
     CDS             complement(11070..11705)
                     /locus_tag="KRL67_002496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH1523366.1"
                     /translation="MKKVKYTRLVILGILIYLVFNILVMIISKNVDTLVLKNEKVDAK
                     IHVKGTIEKDANDQSNYLSTYISKEDSNNFEKNQNVIIEYHEQKLDAKVYKIYENNNK
                     IMVKLKISNQIVANQDTSVEEFDIIYNQMECLKIPKTSIKTKDNKRGVYVIDEQSQSV
                     KFVILEGITYENESSVFVDYYKNDINGVTSVNLYDKIILRPNSINTNIRIK"
     gene            12086..13126
                     /locus_tag="KRL67_002497"
     CDS             12086..13126
                     /locus_tag="KRL67_002497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBH1523367.1"
                     /translation="MSEILLGTGLLLLVLCCFTIFSYKAPYGMKAMGALANAACATFL
                     VEAFNLSLLGDVLGMEFFRSVGAANGSLGGVAAAILVPLALGVSPVYAVLIGLACLDF
                     SILSGFVAGYFVSFLIKFIEKKVPPGVDLVVVILLCAPLSRLIASNVDPLVNSTLLQI
                     GQVLIEATNISPIIMGIILGGLITVVGTSPLSSMALTSIIGLTGLPMAIGALGAFGSA
                     FMNSTLFAKLKLGSKKDIISVAIEPLTQSDIISANPIPIYSTNFFGGAFSGIIVALMG
                     LVNMTPGTATPVAGFAIMFAYNPVQQVLITAGLCALFNVIAGFLGSKIFSTFKVLTAG
                     EIRGTDVEEEIA"
BASE COUNT         4229 a         2252 c         1589 g         5251 t
ORIGIN      
        1 attaaactta atataaaaaa tatataacaa atttttttta tatttctaga agaaaagtat
       61 actctttttt gtttaataat gctaaaattg atttgtattg aattttgatt aggaggaaat
      121 tattatgaaa gctcaaagat taaatgctgt acttgaacaa atgaaaaaag atgatatttc
      181 tcaaatgtta gtaagtgacc ctacttctat attttatctt acaggagtac taatccatcc
      241 aggtgaaaga ttattagcat tatacttaaa tttaaatggc aataataaat tatttataaa
      301 tgaattattc ccagtttcag aagatttagg tgttgaaatg gtttggttta atgatactca
      361 aaatccagta gaaataataa ctgaacatat agacaaaaat gctactatgg gtgttgataa
      421 aaactggcca gctagattct tattaaactt aatagaatta ggtggaggaa gtaagtttgt
      481 aaattcatct tatataatag atactttaag aatgtgtaaa gatgaagaag aaaaagagtt
      541 aatgagaata gcttcaaaat taaatgacaa ggctatggaa caacttaaag ctactgtttc
      601 tggtgaactt acagagaaac aacttgttgg taaattatcc aaaatatatg aagacctagg
      661 tactgatgga ttttcatttg accctattat aggttttgga cctaatggag ctaatccaca
      721 tggtgaacct ggaaatgcac ttgtgaaacc aggagatgca ataatattag atataggttg
      781 tataaaagat aactattgtg ctgatatgac aagaactgta ttctataaag aaatacctga
      841 aaaaggtaga gaaatatttg aaatagttct tgaggctaat aagagagctg aagctattgt
      901 aaaaccaggt gttagattct gtgatataga tgctgctgct cgtgactaca taactgaaaa
      961 gggatatgga cagtacttca ctcatagaac tggtcactct ataggacttg aagttcatga
     1021 taaaggagat gtatcttcta taaatacaga tactgtacaa ccaggaatga tattctctat
     1081 agagccaggt atatatttac caggtgagtt tggagttaga atagaagatt tagtacttgt
     1141 tactgaagat ggttgtgaga tattaaacaa acatgacaaa gaaatatgtg tagttggtta
     1201 attctaaaat caatttgata aaattaaaaa accatataaa aatgttttaa gcatttttat
     1261 atggctttaa tttctcaatc tatgatttat ttcaattcat tatttatatt gcagttatta
     1321 agattgcacc ctttgaagta tatgtcccca tcactgaacc aacttcacag attacaacat
     1381 ctttgtattt tacatccttt tccataactt ccctcacctt ttgagccttt tctgggcaat
     1441 tagcatgtcc tataaataaa accttttctt ctttatcttt aatttttgac tctaccaaag
     1501 taatcaactt tttcaaactg tttccttcgc ctcttgcctt atctatagga gttacaacgc
     1561 catccgcaac ttgtattatc gccttaaaat tcaaggtatt tataattttt cctgctattg
     1621 gatttattct accaccctta atagcatttt ctaaagtttc aagtgttcca aaaaatggag
     1681 gattatcttt atatttattt attattgaaa caatctcttc aatttctttt ccttctttta
     1741 ttaatattgc tgcttttaac actaattgtc ctattccaat tgacccacta agtgaatcta
     1801 taacctccac tttcttgtta tttccttcct tttcaaacat atcttttgct aaaagtgcac
     1861 tattataagt cccagataat cctgaactta tattaatcac caatacactt tcttcctgac
     1921 aatggtatga ttctaaaaat ttatcaggag aaggacatga tgtttttggt agcactttac
     1981 tctcactcat ttttctatag aaagtttcat tatctatttc ctttccagat atataacttt
     2041 cttctccaaa agatacgttt aaaccaacaa cttctatgtt gtatttttct attatatcat
     2101 caggtaagtc acatgcactg tccactatca attttatttt atccatatta acctcactat
     2161 aagatattat tttatttatt cagtcggtcg tacgaccgat ttaattatta ttttatatat
     2221 tgaatgaaaa aaagtcaata gatatagatt ctcgctttat tgacaataaa tattcataag
     2281 cttataatgt tattaatttg ttataaatat acatggaaag gtaggtttta tgaaaacaaa
     2341 taatatgaaa caggctattt tatcatctgc aaccaattta atagccaaaa atggagttca
     2401 aaatactagc cttgctgata ttgcaaaaga tgtaaatata agtaaaggta ctttgtatta
     2461 tcactacgca tctaaaaatg atattatata tgatattgcg gatactcatt tagaagttat
     2521 aactagtgct atactaaact gtgtaaaaaa tgtcaaatca aaaaattctc aaatagaaat
     2581 ggttaactta atcttagaga aaatatctac tatagaaagt cgtggtaggg tacatatgta
     2641 tcttatttgt gaggcaatta cttcaaataa tgatttaaaa gaaagaatga gattaaaata
     2701 tattgaatgg agaactactc ttaaatctga aattgttgaa tcactagaaa aatataatag
     2761 tagcgaaaat gaacaggatg ctgaatcatt ttctttttta ctcatgtcaa tagttgatgg
     2821 tttagttgtc caaagtttat taaaaacaga aaaaatacct tatgaaaata tagcttcttt
     2881 tttagttaat cattggattt aaatttaata cttagataat taataaaagt gaactattta
     2941 agttatctac tttaacagtt cactttctta tttattgtca ataattttta cttaaaatat
     3001 tcaattttta tttaaacctt catggaatca caaaataaaa atctcttata aatttaaaat
     3061 gtttttattt atgttcatgt gtatgcccat ctgaatgttc atgaccatgt gtatgtgtat
     3121 gctcatggct atgttcatga gtacctgaaa aaccatcatc atgattatgg tcgtgaccta
     3181 caccatttac atgctcatgt tcatgcccat gactgtgaga atgttgatgt gaatgttcat
     3241 ggttatctcc tccacaacat ccttctcctc cagtcccaca acatccactt gtatgtggat
     3301 tatgatgatt tcttactaca aatctctctc ttaacataat tcttatacct cctagtatat
     3361 tattgagtac ttcatgtacc ccattaacta cagtatacat ctaactaggt ataatctcaa
     3421 tacatatggt attttttaac atatacaatc ttaaagaaat ttatccttaa agcctccacc
     3481 ttttacttct tcaacgttat tgacagaaat aaaagcttca ctatccatat cgtaaattaa
     3541 atctttaaat ttacctattt cgtttgcaga tattatacaa taaatcattt ttttcttatt
     3601 ttgtgtatat gcaccttctg cctccaaaaa agtgactcct cttcccattt tattcattat
     3661 cactttggat atttcatcat atttatttga tattaaaagt atggattttt ttctatcaaa
     3721 gcaatctttt cctatatcca tagtaaaaga aattatatac attgtaatta aagtgtacat
     3781 caccaattta gctccaaata agaaagctcc tgcacatact acaaaaaaat taataaataa
     3841 aaatgtattt ttcataggta tatcatactt tcttttcatt atggctgcta ttatatcaag
     3901 tcctccagca gtagctcttg ctttaaaagt taaccccata cctataccag ttaatgcacc
     3961 accataaata cactgtagca tagtatcatc tattggaaaa tatttggcaa tatcttgtgt
     4021 tagccccagt gctagagaaa atagtaacat attgacaaaa cttattagca aaaatttatt
     4081 atcaaaatac tttctagcta taataaatat tggtatgttc atcaagaaac ttgatattcc
     4141 ctgatttata ccaaataatt tgtataatat aacacaaatt ccagctactc ctccacttaa
     4201 taacttggct ggtgctagat ataaatttat tcctaaggtt gatagaatca tacccaatat
     4261 aataagacaa tattgtaaaa atatgttatt atcaatactt agctttttac tttcaccata
     4321 catcaaaacg cacccctttg cataataaat aaaccaccct gcagaacaca cgataaattt
     4381 ataaataaat ttatcagaag tagcttacta aaaatattat tcatcctcaa ttaaacgttt
     4441 attcattaat attctatcac atgacataaa taatttatat gatttatata gatttattta
     4501 ttgtaaattt tggaatatta aaacatttta ttgaatagat aattatatta cataaaaaat
     4561 ccctatagaa atctataaac taatttctat agggattttg gttaactaac tattcttttt
     4621 atttttttat aacttttata cctgtcatat ggtcatttaa gttttgttcc tctaaagatt
     4681 tatcatctac tctgtttatt tctaaagcta aagtttcaga cttgatgtat tcatcaaaat
     4741 ggtcaacagc cttagcaatt tcatcatcac cacagtattc tattactata ttatctaata
     4801 cttcaaagtt attagatttt cttaattgct gtactttaga tataaattct ctagcataac
     4861 cttcattaat caactcttca gttagcttag tatctagtat aacaaaaaga ttattttcaa
     4921 cagatacatt aaatccttcc ttagcagtta tacctattaa tactaaatcc ttattaaatt
     4981 caaaagcttc accatctaaa tcaactgtta aagtctcacc tttttcaagt ttattagcag
     5041 tttcatgagc atttaattta cttaatgctc caacgaataa attcatctta gaacctaata
     5101 atggtcctgc ttccttaaag tttggtttta aactaaagtt catatactca tctaaatcct
     5161 tagcaaatat tacctcttta acattaagct cttcttttat taaatcaact acatcactta
     5221 ttgtatcttc aaacttacca tctactaaca ctttttgtaa tggttgacga acttttattc
     5281 tctctacttc tcttgaagct ctacctaaag taactaagtt tttagctaaa tccatttttt
     5341 cttctaattt agtatctact aactcttcat tacacttagg atattcagct aaatgaacag
     5401 atacttctcc tgttaagttt ctatacattt cttctgacat atatggtgca aatggagcta
     5461 tagctctaca aagaccatgt aacaattcat aagttgtatt gtacactgct tttttatctt
     5521 ctgttaattc agttgcccag aatcttcttc ttgaacgtct tatataccag ttagataaat
     5581 catcatttat aaaatcttgt atcattctta aagttttatt taactcaaat atagctaaat
     5641 tttcttctac atctttcatt aaattattga atttagataa tatccatcta tcaagttctg
     5701 gtctatcttt atattctaca aaaaattcag ttggatttat atcatctgtg tttgcatata
     5761 aagtaaagaa attatataca ttcttcatag tacctataaa tttactttgg atttctttta
     5821 atccatccat atcaaattta gttggagtcc atggtggaga aacatatagt aaataccatc
     5881 ttaatgcatc tgctccatat tggtcaaata attccattgg atttactgtg tttcctcttg
     5941 atttactcat tttcttacca tctttatcaa gtacaaggtc tggaactaaa acactcttat
     6001 atggtgcttt tcctaataca aatgtcgata ctgcaagtaa tgagtagaac caacctctag
     6061 tctggtctat accttctgat atataatcag ctgggaacag ttggtcaaag ttttccttat
     6121 tttcaaatgg atagtggtgt tgagcaaatg gcatagcccc actatcaaac caacagtcta
     6181 taacctctgt tactcttgtc attggttttc cacacttttc acattttaag tgtatatcat
     6241 ctacatatgg tctatgaagt tcaactgttt ctggatttac atcttctata gctttttcag
     6301 ctaattcagc tcttgaacca actgaatctg tatgaccaca ttcacatctc catataggaa
     6361 gtggagttcc ccaatatcta gttcttgata ttgcccagtc atttagattt tctaaccagt
     6421 ttccaaatct cttttctcct acaaaacttg ggaaccattt tacaccatta ttattttcta
     6481 ttaatttatc tttaagttta gtcatctcta tataccagct tggttttgca tagtatacta
     6541 gtggagtttg acatctccaa cagtgtggat aattatgaac tactttttct ttgctatata
     6601 atttattttc agaagctagc cattttatta tttcaacatc tagcccatct tccattacaa
     6661 atctaccttc ccaaggagta gatgtaaatt taccagtttc atctactggt tgaataactg
     6721 gtaaatcata tcttttacct gtgttgtagt catcttcacc aaatgctggt gctgtatgaa
     6781 ctataccagt accatcttca gtagttacat aatctcctac tgtaataaaa aatgcttttt
     6841 tgtcaacttc tacaaaaggc attaattgtt catattctaa accttctagg tctttacctt
     6901 tcatttcttc taaaacttca tagtcttcac ctaatacttt attagctaaa ggtttagata
     6961 cataatatac ctcatcacct tttttaactt ttaaataatc tacatcagca tttactgtta
     7021 acgaaacatt tgatggtaga gtccatggag ttgttgtcca tgctaagaag tattcatcag
     7081 catctttttt cttaaattta gctataactg tattagtttt tacttcttta tatccttgtg
     7141 caacctcatg actagcaaga ccagttccac atcttgggca ataaggaagt attttatgcc
     7201 cttcatagat atatccttct ttgttgaatt tattcagtat ccaccagaca gactctatat
     7261 agttattatc taaagttata tatggattat ctaaatcaac ttcataagcc attctttcag
     7321 tcattattct ccattgtttt tcaaatgaga aaactgactc tctacatttt tggttaaatt
     7381 cagctattcc atactcttct atttgttgct tgctagtaag acctaactct ttctctactt
     7441 gtatttcaac tggtaatcca tgtgtatccc aacctgcttt tctctttact tgataaccag
     7501 acattgtttt atatctacaa actgaatctt taagtgttct agataaaaca tggtgtactc
     7561 caggatttcc attagctgtt ggaggtcctt catagaatac aaaacttggg tcatctttcc
     7621 ctttttctaa agttttttca agtatattta tgtctttcca ataatcaaaa acttgtgctt
     7681 ctgcttgttt tacagaactg tctaccaatg gcttaaattt cgccatgtaa atcacccttt
     7741 cctataaata ttataattcc aaaatttttt attctttcac ataaatttaa atactaaaaa
     7801 actcgccccc tttaaaccaa agggacgagt tataaatcgc gttaccaccc taattctata
     7861 tgtaaactaa atactagcta catataacac tctaatagta taacggtact aaccggcaca
     7921 gcttagtaga atttaatcgt tcagcctgca gctcaagagt gatttttatc tttataaatt
     7981 cattggttct cagcataccc aattctctgt aaaattatct aaagataaat gtctctatca
     8041 acgcttttaa aaatttatct gtgtttatat gaaattctat aacaaataat ttgaatagtc
     8101 aatatatttt tgaaaaatat aataatttct atttttctta catttttatc ctatttttaa
     8161 tctttgttat agatttctca aacttacaca atgattctac ataaaaaata aacaaattac
     8221 aagcttattc taaagttgta gcagcttcat cattactata attatacact gttgttcctt
     8281 caaatgcatt tacacatttt tcatctacat cataaaaaat ctcatcaaca cttcttattt
     8341 catcttccaa cagtgattta aatttatttc taaatatctt aaattctttg actaaagcat
     8401 catactcttt tcttatttct ataacacggt tattagcttg ttctataatt tgtctagctt
     8461 ttaaatcagc ttcttcaaca attatcttag cctttttatt agccgcgcta caagtgtctt
     8521 ctgctgcact ttgagcagtt attaaagtag cttttagtgt ttcttcaata ttttcatact
     8581 tgtttacttg gtcttgatat aaccctaatt tttctttaag agctgtattt tccctacata
     8641 gagattcata atcttccttg atgatatcta aaaattcatc aacctcttca tctctatacc
     8701 ctcttaaacc ttttttaaac tctttatttt ctatctcaat tggagttagc attacatttc
     8761 ctcccagcta taatttaaat tagtccttat acaattaatc ttgcttgaac ttttattttt
     8821 ccttttttag taatatctcc tatttgtgtt accttagctc ttcctttgcc tctaatagaa
     8881 atcaaatcat cattttttac tattttagaa gttgaagtta tcttttcata attgacatgg
     8941 actttttctc catttatgta ttttgcacta tcttgtctag atatattgta aataccactt
     9001 atcacacaat ctaacctatc tgaagatact gtaaatgata cttctttata ttttgaagaa
     9061 ctattaacaa tattttctct ggaaatttcc tttacaatta cattatttct tgaaaccttt
     9121 tctaagttta caattataaa atcgcaaata tcactactta caactacttg acaaaagcta
     9181 tcatgtataa ttatatctcc aattttttct ctttttatac ctagactcag tattgaccct
     9241 aagtaatctt tatgagatat atttttaaat ttgaaattcc cttctatttg taaaaatcta
     9301 agagtatctt ctatatcttc gtattccatg taaaatggat aaataaatac agtgcttctt
     9361 tcagcttgtt cataacctcc atccacactg tattttattt catttgtaga atttaatatt
     9421 gctacagcat ttttcacttc atatggatta agaaactctg tagacttaac atcatagttt
     9481 tttatacaac tattagcctt gtctatcact ttaaacattt tatttttcaa gtctatatcc
     9541 tttatgtgat ttgttagttt taatttatcc atcctatcac cttagaatat aaccaatact
     9601 aattgtctta ggaacatcaa taatactatt gcaattaaag gagaaaaatc tataggtcca
     9661 tttgtatatt tatacattat agaccttatt ggcccttcta ttggctctgt aagattttcc
     9721 aaaatatcaa aaattttact ttgtcttcca tttggaaacc aagtcatcaa gctttttata
     9781 actatcatcc atgctattat atctaaaaga taataaagtg caattcttat tgttcccata
     9841 tattaaccgc ctttttacta tttattttgc caagggaaga atgctttact ttctaattct
     9901 tttttcatat ttgcatctat atccacatta ttaggagcta gtataaatat tgatttagaa
     9961 accttttgta tacttccatc taagacataa actgccccat tcataaagtt aaatatagat
    10021 ttacgaactt cagaattagt caatccttcc aaattcacta taactgccct tctttgtttt
    10081 agatggtctg ctatagttgc agcttcatca taaaccttag gttcaactat tacaactttc
    10141 atttggctag ttgtatgtaa atttacaatt ttatttgctt ttgcagtact aaagccactg
    10201 ttcatttctt cttcttgaac tatatcgtcc atgccacttt catattcatc ttcaatgtaa
    10261 tcttcttctt catcaactat ccaattctta aatttagata ttattccatt tgacattatt
    10321 aacttcctcc ttaaaattta ttcaaatttg agtaattatt ttttattgta gtttctttct
    10381 ccaaagattg atgttccaac tctaacaatg gtagcccctt cctctatagc aatttcataa
    10441 tcgtgactca ttcccattga caaaacatcc atccctacat tagggatgtt tagttctttt
    10501 atttgttttg ataaatcttt aagaccttta aatacacctc ttatctcatc ttcatcttct
    10561 ataaaaggtg ccattgtcat tagacccttt atttttatat ttttatattt ttctgacact
    10621 tctttgacaa agtctacaac ttcttctcta tcaagtccat gcttagattc ttctttagat
    10681 atattcactt gtactaagca atctatttct ctgtttattt gttctgcacg tttttgaatt
    10741 tcttcgcata atgcaattct atctaaagag tgtatcatac aaactttatc gattatgtac
    10801 ttaactttat tggtttgcaa agttcctatc aggtgccagt ttaatttgtc acctataata
    10861 tcatactttc tagctaattc ttggggttta ttttcaccaa cagaagtgat tccttccctc
    10921 attgcctcat taactaagtc tacatcaaca gtctttgtga ctgccaaaag ggtaatctct
    10981 ttgctgtctc tatctacttt ttttgcagct ttatcaattt tgtttcttat ctcagacaaa
    11041 ttatctttaa tagaacccat tattatcacc tactttatcc ttatatttgt gtttatactg
    11101 ttaggtctta aaattatttt gtcatataga ttaacacttg taactccatt tatgtcattt
    11161 ttataataat ccacaaatac agaagattca ttctcataag ttattccttc taatatgaca
    11221 aattttacac tttgtgattg ttcatcaatt acatatacac cacgtttatt gtcttttgtt
    11281 tttatagaag ttttaggtat ttttaaacat tccatctgat tatatattat atcaaattct
    11341 tcgacacttg tatcctgatt tgcaacaatt tggttactta tttttaattt taccattatt
    11401 ttattattat tttcatatat tttgtaaact tttgcatcta atttttgctc atgatattca
    11461 attattacat tttgattttt ttcaaagtta ttagaatctt ctttactgat gtatgtagat
    11521 agataattac tttggtcatt ggcatctttt tctatagttc cttttacatg aatctttgca
    11581 tcaacttttt cattttttaa caccaacgta tctacatttt tgctaattat cattactaat
    11641 atattaaata ctaaatatat aagaatacca agtatcacta atctggtata ttttaccttt
    11701 ttcaaagact ctccccctat aaaattactt tcattataag tacttcttct aaaaaatgtt
    11761 tattcctttt ttataagcta atattttacg ttctaataat tattttttcc atattaaatt
    11821 tattttttat caatcttcct atacaagact gcattattta tatataattg tattataaag
    11881 tataagttta atttatattt tatacgttaa gcttacttta tatgttttta tagttatttt
    11941 tatttttaga tgcaatataa aaccaattaa gatttaaaat aacttttgtt ctgttgacat
    12001 ttatttaggt aattatttac gaatcaggat aacgttatca tggcaaaagt ttatgtccta
    12061 aaaaaaatac aaggaggatt ttcttatgtc agaaatttta ttaggaacag gtttactttt
    12121 attggttcta tgttgtttca caatttttag ttacaaggcg ccctatggca tgaaggctat
    12181 gggagctcta gcaaacgctg catgcgctac tttcttagta gaggcattca acctttcttt
    12241 acttggtgat gttttaggta tggaattttt tagaagtgtt ggagctgcta atggtagtct
    12301 tggtggagtt gctgctgcta tacttgtgcc tttagcctta ggtgtatctc cagtttatgc
    12361 agttttaata ggattagctt gtttagattt ttctatatta tctggattcg ttgctggata
    12421 ctttgtttct ttcttaatta agtttataga gaaaaaagtg ccacctggag tggatttagt
    12481 ggttgtaatt cttctatgtg caccactatc tcgtcttata gcatcaaatg tagacccatt
    12541 agttaatagt acactactac aaattggaca agttttgata gaagctacca acattagccc
    12601 tataataatg ggaattatac taggaggtct aataacagta gttggaacgt ctccacttag
    12661 ttcaatggct ctcacatcta taattggtct tactggtctt cctatggcaa taggagcatt
    12721 aggtgctttt ggttcagctt ttatgaactc aacgctattt gcaaaattga aattaggttc
    12781 taaaaaagat attatatctg ttgctattga accattaacc caatcagaca taatttcagc
    12841 taatccaata cctatatatt ctactaattt ctttggagga gccttttctg gtatcattgt
    12901 agccttgatg ggtcttgtaa acatgacacc aggtacagct acacctgtgg ctggttttgc
    12961 tataatgttt gcttataatc cagttcaaca agttcttata acagctggtt tatgtgcttt
    13021 attcaatgta attgcaggat ttttaggttc aaaaatattc agtaccttta aagttcttac
    13081 tgctggtgaa atacgtggta ctgatgtaga ggaagaaatt gcttaaaaaa caataagatt
    13141 tataaattta tatctcaagt agtagagttt tatattgtag gcaattttat taattgcaaa
    13201 cgttttattt tttatttatg tttatgatta ttatgtgtgt tgaatattta gataaatttt
    13261 tatttattat ttttatttag ctttttgttt agttttttat ttggttgata tattaggaat
    13321 a
//
LOCUS       DAEPXF010000100        13249 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain HB27
            SAMN13376389-rid12479423.denovo.106, whole genome shotgun sequence.
ACCESSION   DAEPXF010000100 DAEPXF010000000
VERSION     DAEPXF010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13376389
            Sequence Read Archive: SRR10518432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 13249)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13249)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001069624.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001069624.1
            Genome Coverage       :: 315x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/15/2021 01:54:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,165
            CDSs (total)                      :: 4,076
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 89
            rRNAs                             :: 10, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 10 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 19 of 69
            Pseudo Genes (incomplete)         :: 44 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 10 of 69
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13249
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13376389-rid12479423.denovo.106"
                     /strain="HB27"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="China: Hebei"
                     /collection_date="2017"
     gene            125..1948
                     /locus_tag="KRL67_002498"
     CDS             125..1948
                     /locus_tag="KRL67_002498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HBH1523368.1"
                     /translation="MKRFLRRIILVLFILLIFISFISIKLIHNVGDYGKLINYVGIVR
                     GASQRLTKLEMNHHPNDELIEYIDEILQELITGHGDYGLVITDCNEYNEDLLLLEKKW
                     EDLNIEIKKVRMKEQNNQLLSISEEFFSLANDTVFKIESFSKEKSNYLMTLIIIISII
                     GILACIILILQYSKKMIRLEKLNVDLKNIAYKDELTGVNTIEKFKLDANQNICMHKDK
                     KFAVFYIDFENFKYINDIFGYDYGDMILKRYANLMMNDIGKYEIFAREIADRFVALRC
                     YVDKEDLVVRQRDVDNELINITNEIKNKHSITVVSGICCIEDVNEKLSIDGLINRANF
                     AQKTVKNKPDTNYAFYNDSIRKKMIEENTIKSRIHEAIEKREFIVYLQPKVNLHNQKI
                     NCAEALVRWLTPDKGIISPVIFIPVLEKNFFIALVDKYVFEEVCKWIRKRLDENKPFV
                     QISVNVSRIQFYNTKFVETYSNIQNKYRIPKNTIEIEFTESVAFENQKHLLEIIHDLH
                     ENGFTCSLDDFGKGYSSLSVLKDLPFDVLKLDSMFFKASLDKDKEKIVIKNIVQMLKE
                     LNITTVAEGIECKEQVEFLRDIGCDLVQGFVFYKPMPILEF"
     gene            complement(2276..2875)
                     /locus_tag="KRL67_002499"
     CDS             complement(2276..2875)
                     /locus_tag="KRL67_002499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerD family protein"
                     /protein_id="HBH1523369.1"
                     /translation="MAIELKKGQKINLTKKENSNLGEILVNLNWNQKVQKKGFFGSLR
                     SSNIDLDLGCLFEMKNGVKGAVQALGNAFGSLDNPPFAQLDGDDRTGSNTQGENLRIN
                     GNKIKDIKRILIYAFIYEGVANWSEADGIVTIKQKQDSDLVVKLDEHKNGYNMCSIAL
                     IENVNDETFSVEKVVKYFKGHREMDTEFHWGLKWVAGRK"
     gene            complement(2978..3775)
                     /locus_tag="KRL67_002500"
     CDS             complement(2978..3775)
                     /locus_tag="KRL67_002500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HBH1523370.1"
                     /translation="MKFKKLLCLLLCLVLTLAVVGCSKAKDDKKIVVGATLVPGGELL
                     EELKPLIKEKGYTLEVKNFDDYILPNEALNNGEIDANLFQHEPYLKEAVKAKGYKIMA
                     GKKLYVCPAILYSYKIKSVDEFKKGDTIAISNNPSSCSKNLRYLESIGLLTLPKGDGL
                     VSPKDIIENPKGIQFKELDIAQIPSSLPDVTAAFIDTTYAVPAGLDAKKNGIYTAPIN
                     DEYANLLAFRTEDKDSEKIKVLQDVLTSDKARSLIEEKYKGIVIPTF"
     regulatory      complement(3888..3993)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            complement(4085..5071)
                     /locus_tag="KRL67_002501"
     CDS             complement(4085..5071)
                     /locus_tag="KRL67_002501"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HBH1523371.1"
                     /translation="MLLIYNEKTNPYFNLAMEEYLLKNFDEDLFILWRNEPSIIVGKN
                     QNTLSEINLEYIKEYSIPVVRRQSGGGAVFHDLGNINFTFIACNNNNFSDFRRFTQPI
                     IDLLKTLDVNAEFSGRNDLLIDGKKFSGNAQYNYKNKVMHHGTLLFSSEINDLSKALK
                     VKPIKFEGKGIKSVKSRVTNISEHLHSKMDILEFKDAIMDYLSKTNTDNTNYCLSEHD
                     IKQIEKLTKSKYETWDWNFGNSPKYTLSNELKYSGGNVEFNLNVDKGIITSIKFFGDF
                     FGKCDVAFVENKLIGIKHEENALRNTLDSIEINDYFLGADTDILVSGILGVK"
     gene            5554..7284
                     /locus_tag="KRL67_002502"
     CDS             5554..7284
                     /locus_tag="KRL67_002502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC family protein"
                     /protein_id="HBH1523372.1"
                     /translation="MRNKKINIIFLTTIMFIMSTVMVFAEEDIDTIALANAEKFGILT
                     LIPPLVAIILAFITKNVIISLLIGILSGSFIIKASGINVFATFIQAFLDLVDRALVSL
                     ADPWNAGIILQVLAIGGVINLVAKMGGAKAIAEALAKRAKSAKGTQLITWFLGLLVFF
                     DDYANSLIVGPMMRPVADKMKISREKLAFIIDATAAPVAGLAIISTWIGLEVGLIHDA
                     FESISIDVDAFGIFLNTIPFRFYNILILAFIVISALLLKEFGPMRKAEIKSRSRKISI
                     DLDEGVAELDDLAPKNGVKLSVWNAIIPIGTLIIVALASFYYSGYTSIMGGDDKALIQ
                     LFTNSPYSFEAIKEAFSASDASRALFQSALVASLVAIIMAVVKKIFTISEAIDVWIDG
                     MKSLVITGVILILAWSLSSVIKELGTAKFLIHLLSGSLPPFLLPSLIFGLGAIISFAT
                     GTAYGTMGILMPLAIPLAYSLNPDMSYVIVSTSAVLTGAIFGDHCSPISDTTILSSMG
                     AGCNHIDHVNTQMPYAIFTAVITIVFGYIPAGLGLPIYIVLPVAIAAIFIGIQIIGKR
                     VDEAEIELVE"
     gene            7394..7474
                     /locus_tag="KRL67_002503"
     tRNA            7394..7474
                     /locus_tag="KRL67_002503"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7428..7430,aa:Leu,seq:tag)
     gene            7809..8324
                     /locus_tag="KRL67_002504"
     CDS             7809..8324
                     /locus_tag="KRL67_002504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HXXEE domain-containing protein"
                     /protein_id="HBH1523373.1"
                     /translation="MEKYIWLFPLLFIFHDMEEIIGFGIWLKKNKSMLDKKYPFISKI
                     YENYSTEGMAFAVFEEFILCIIFCILIVITDNQYVYLLWLGSFIAYTLHLVIHIGQSI
                     IIRKYIPSLITSIICLPISIWCISKSIYIVDCEMSTTILYSIIGIIIVALNLKFAQSL
                     IGKFTKWMSNI"
     regulatory      8800..8904
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00504"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="glycine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="glycine"
                     /db_xref="RFAM:RF00504"
     gene            9114..11588
                     /gene="gcvPA"
                     /locus_tag="KRL67_002505"
     CDS             9114..11588
                     /gene="gcvPA"
                     /locus_tag="KRL67_002505"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPA"
                     /protein_id="HBH1523374.1"
                     /translation="MNELKRVSLYNIHKELGAKLVEFAGWEMPLEYEGINKEHEMVRK
                     SAGIFDVSHMGEVQIKGVESEKFIQNLVTNDISTLNINDIIYTPMCYENGGVVDDLLI
                     YKFGEEEYLLVINAGNIDKDVAWIIKQSEGYNVDIKNISSEVSQLAIQGPKAEEILQK
                     ITDIDLNSIKFYKSIPSTKVCGCPCLVSRTGYTGEDGFEIYCKNKYVEIIWNEVLKVG
                     GEDICPAGLGCRDTLRFEAALPLYGHEINEHISPIEGGLSIFVKTNKESFIGKSILSK
                     EKESGAKRKLVGFEMQGKGMPRNGYDIRIGDKIVGFVTTGCASPTTGKILGMGIIDSE
                     YAKVGNEIGIAIRKKVVPAVIVKKPFYKKQYKKDNIILNKENKFSYIPATSEDKSKML
                     KVVGLNSVDELFSDIPEEVKLKRDLNLEIGKSELEVSKIVKRLSEENLSLEDLTCFLG
                     AGAYDHYIPSIIKHITSRSEFYTAYTPYQAEISQGTLQVVFEFQSMIAEITGMEIANA
                     SMYDGATAAIEACIMAINQTRKSKIVVSKTTHPETLSVLRTYLQYKDCEIVEIDFCNE
                     YGTTDIEKLKASVDKDTACVLIQTPNFFGIIEEMEEIEKITHENKAMLIMSVDPISLG
                     VLKTPGEIGADIVVGEAQSLGNPLNFGGPYVGFLASKSKYTRKMPGRIVGQSLDVEGK
                     IAYVLTLQTREQHVRREKATSNICSNQALNALVASIYMATMGKEGFKEVGMQSMKKAH
                     YTYNKLVQTGKYKPIFKGKFFKEFAVQGNLNIETINDKLLEENILGGYNLEYNYPELK
                     NSTLLCVTEKRSKEEIDKLVGIMEGL"
     gene            11588..13045
                     /gene="gcvPB"
                     /locus_tag="KRL67_002506"
     CDS             11588..13045
                     /gene="gcvPB"
                     /locus_tag="KRL67_002506"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPB"
                     /protein_id="HBH1523375.1"
                     /translation="MKEYNSLLIDISKKGRKAYSLPKLDIDDIKIEDMIDQDMARQSE
                     LNLPEVGELELVRHYTLLSNKNFGVDTGFYPLGSCTMKYNPKINEDMAALPNFTGMHP
                     YQSSDTAQGSLSLMYDLSRRLAEITGMDEVTLQPSAGSHGEFTGLMIIKAYHENRGDH
                     KRTKVIIPDSAHGTNPASAAMANFDVIQIASDKNGAVDINALKEVLNDEVAALMLTNP
                     STLGLFEKNIKEIATLVHEAGGLLYYDGANMNAIMGITRPGDMGFDVVHLNLHKTFST
                     PHGGGGPGAGPVGVKKELVPFLPIPIIERKEDKYVLNYDREKSIGKIKNFYGNFGVLV
                     RAYTYILTMGRDGLKEASEMAVLNANYIKESIKDDYILPIDTLCKHEFVLGGLPRGEA
                     NIKTIDIAKRLLDYGYHPPTMYFPLIINEALMIEPTESESIETLDSFIEAMKSVAKEA
                     KEEPELLKTAPHNTLVKRVDDARAVKKPILTWSQR"
BASE COUNT         4956 a         1575 c         2197 g         4521 t
ORIGIN      
        1 aaacaaggtt tatatattct aattgtatag aaaattttat tggagataca tgtatattat
       61 tagaaaaaat agtttagaga tattcagtaa acataaaaaa taatgtaggg gataaaggta
      121 ttttatgaag agatttttga ggagaattat cttagtttta tttattttat tgatttttat
      181 aagttttatt tctattaaat taattcataa tgttggagat tatggaaaat tgattaatta
      241 tgttgggatt gttcgtggag ctagccaacg tctaactaaa ctagagatga atcatcaccc
      301 aaatgatgaa ttaatagaat atattgatga aatcctacag gaattgatta cagggcatgg
      361 agattatggt ttagtaatca ctgattgtaa tgaatataat gaggacttac ttttattaga
      421 aaaaaagtgg gaagatttaa atattgaaat taaaaaagta agaatgaaag aacagaataa
      481 ccagttatta agtataagtg aagagttttt ttctcttgca aatgatacag tgtttaaaat
      541 agaaagcttt tctaaagaaa aaagtaatta cttaatgact ttaataataa ttatatctat
      601 tataggtata ttagcatgta tcatattgat tttacagtat agtaaaaaaa tgattagact
      661 ggaaaaatta aatgtagatt taaaaaatat tgcatataaa gatgaactaa caggagtaaa
      721 tactatagaa aaatttaaat tggatgcaaa tcaaaatatt tgtatgcata aggataagaa
      781 atttgctgta ttctatattg attttgaaaa ctttaaatac attaatgata tatttggata
      841 tgattatggt gacatgatac tgaaaagata tgctaatctt atgatgaatg atatagggaa
      901 atatgaaata tttgcaagag aaattgcaga cagatttgtt gcgttaagat gttatgtaga
      961 taaggaagat ttagtagtaa ggcaacgaga tgtagacaat gaactaatta atataacaaa
     1021 cgaaattaaa aataaacata gcattactgt tgttagtgga atttgttgta ttgaagatgt
     1081 aaatgaaaaa ttaagtatag atggtttaat aaatagagca aattttgcac aaaaaactgt
     1141 taagaataaa ccagacacta attatgcttt ttataatgac agtattcgta aaaaaatgat
     1201 tgaagaaaat actatcaaga gtagaataca tgaagctatc gaaaaaagag aatttatagt
     1261 ttatttacag cctaaagtta atttacataa tcaaaaaata aattgtgcag aagctttagt
     1321 tcgttggctg actccagata aaggtattat atctccagta atatttattc cagtacttga
     1381 gaagaatttt tttattgcct tagtagataa gtatgttttt gaggaagttt gtaaatggat
     1441 tagaaagagg ttagatgaaa ataaaccttt tgttcagata tcagttaatg tttccagaat
     1501 acaattttat aatactaaat ttgtagaaac ttattccaat atacaaaata aatacaggat
     1561 acctaaaaat actatagaaa ttgaatttac agagagtgtt gcttttgaga atcaaaaaca
     1621 tcttttggaa attatacatg atttgcatga aaatggattt acttgttctt tagatgattt
     1681 tggaaaaggt tattcgtctt taagtgtttt aaaagattta ccatttgatg ttttaaagct
     1741 tgactctatg tttttcaagg ctagtttaga taaagataaa gagaaaatag taataaaaaa
     1801 tattgtacag atgttaaaag agcttaatat cacaactgtt gctgagggca ttgaatgtaa
     1861 ggaacaagtt gaatttctta gggatattgg gtgtgattta gttcagggat ttgtatttta
     1921 taaaccaatg ccaatattag aattttaaga aatattggat aaagagtttg tttataatag
     1981 ctaaaatgta ctttgtaaaa tacgaactaa ttaagtaaaa ttattttgta ctaaataaat
     2041 ttttacttta tttacagaat attgattatt tacagaaaag cgagaaaagt gtgttccttt
     2101 aagtgtggga tggatagcac aaataggata taaaaaatat tgaccatata agaatgcata
     2161 atgatataaa tatttgaaag gttaccatta tatgaaaatt aaaaagaaaa taaataattt
     2221 ttcgataaaa aagagactat gagaaaagtt actcatagtc tcctaaataa tgtatttatt
     2281 ttcttcctgc aacccatttt aagccccaat gaaattcagt atccatttct ctatgacctt
     2341 tgaagtattt aactactttt tcaacactga atgtctcatc atttacattt tctatcaatg
     2401 ctattgaaca catattatag ccatttttat gttcatcaag tttgacaact aagtctgaat
     2461 cttgtttttg tttgattgtt acaattccat cagcttcaga ccagttagct acaccttcgt
     2521 atataaaagc ataaataagt attcttttta tatcttttat tttattacca tttattctta
     2581 aattttctcc ttgtgtatta cttcctgttc tgtcatctcc atctaattga gcaaaaggtg
     2641 gattgtctaa actaccaaag gcatttccta atgcttgaac agcacctttt acaccatttt
     2701 tcatctcaaa taggcaaccc aaatctaaat ctatattact gcttcttaaa gagccaaaaa
     2761 aacccttttt ttgtactttt tgattccaat ttaaattaac taagatttca cctaaattgc
     2821 tattttcctt ttttgtaagg tttattttct gtcccttttt taattctata gccatagtaa
     2881 tcttcctcca caaacataga tttatttaaa tggtagcact taataagtca atattcaaca
     2941 agattagaat aataatatta aaattatact aaatatacta aaaagtagga attacaatac
     3001 ctttgtattt ttcttcaatt aagcttcttg ctttatctga tgtaagaaca tcttgtagaa
     3061 ctttaatctt ttcactgtct ttatcttcag ttctaaatgc taaaagattt gcatactcgt
     3121 catttatagg agctgtataa attccgtttt tcttggcatc aagaccagca ggtacagcat
     3181 aagtagtgtc tataaatgca gcagtcacat ctggaagaga agatggaatt tgagcaatat
     3241 ccaactcttt aaattgtatt ccctttggat tttctattat gtccttagga ctaactaatc
     3301 catccccctt aggtaacgtt aaaagtccaa tactttcaag gtatctaagg ttcttagaac
     3361 aagaagatgg gttattgctt attgcaattg tatctccttt tttaaattca tcaacagatt
     3421 ttatcttata agagtaaagt attgctgggc atacatatag ttttttacca gccattattt
     3481 tgtaaccctt ggccttaaca gcttctttta gataaggttc atgttgaaat agatttgcat
     3541 caatctcacc attatttaaa gcttcgtttg gaagtatgta atcatcaaag tttttaactt
     3601 ctaaagtata tcctttttct ttaataagtg gttttaattc ttctaataac tctcctcctg
     3661 gaactaaagt agcaccaaca acgatttttt tatcatcttt agctttagag catccaacca
     3721 cagctaaagt taatactaga cataataaca aacataataa ttttttgaat ttcatataaa
     3781 ttccctcctg aatataattg attagttaaa aataaaatac aattctttat cacactgcaa
     3841 aataaaaata agcctgagtt taaactcagg ctttaaaagc taatttagac tcatctttca
     3901 gtataatact gcaggattta gcaccaatgt atataaaaat atactggttg ctgggtttca
     3961 tagggccagt cccttcacca ctctggataa gaagtatcaa tttttaatta tgttataaat
     4021 attatcatga ttttttacgt ctgtaaacat aattttataa atattttaaa atctttaatg
     4081 ctttttactt aacacctaaa attccagaaa ctagaatatc agtatcagca cctaagaaat
     4141 agtcatttat ttcaatagaa tccagagtat ttctcaaagc attttcttca tgctttatac
     4201 caataagttt attttctaca aatgcaacat cacattttcc aaaaaaatct ccaaaaaatt
     4261 ttatactagt tattatgcct ttatcaacat ttaaattaaa ctcaacattt ccaccagaat
     4321 attttaattc atttgaaaga gtatattttg gtgaattacc aaaattccaa tcccaagttt
     4381 cgtattttga tttagtaagc ttttctattt gttttatatc atgttcactc aaacaataat
     4441 tagtattatc agtatttgtt ttagaaagat aatccattat agcatcttta aattctaata
     4501 tgtccatttt agagtgtaaa tgttcactta tattggttac acgagatttt acagatttta
     4561 ttccttttcc ttcaaatttt ataggtttaa cttttaatgc ttttgagagg tcatttattt
     4621 cagatgaaaa taaaagtgta ccatgatgca ttactttgtt tttgtagttg tattgagcat
     4681 ttccagaaaa tttcttgcca tctataagaa ggtcatttct tccagaaaat tcagcattga
     4741 catctagtgt tttaagtaaa tctattatgg gttgagtgaa tcttctgaaa tcactaaaat
     4801 tattattgtt acaagctata aatgtaaaat ttatatttcc taaatcatga aatacagctc
     4861 caccaccaga ctgtctccta acgacaggta tagaatattc ttttatatat tctaaattta
     4921 tttcagaaag tgtgttttgg ttttttccaa caattataga aggttcattc ctccataaaa
     4981 taaataagtc ttcatcaaaa ttttttagaa gatattcttc cattgctaaa ttaaaataag
     5041 ggtttgtttt ttcattataa attaataaca ttaaagtagc tcctccatat aattgataaa
     5101 atttttatta tgaatacaat taattttata attcgacaat aatgtaaaaa ctcctttaaa
     5161 ttagaaattt taaaaactat cttgcaaagt ttaaataaat aatatatact caaaatatgt
     5221 ttaataaaag tgaatagaag ggtagaggcg caataatcta tcagtactaa taaggagtta
     5281 agcacagaga gttattagga aaggagatat tgccgaagtt ttataatcga tgctttaaga
     5341 ttatattgct gggggtacat agaaaatatg tacaactgtc acaggtttat ttgtggtgag
     5401 ctatcattta aggaattata ttatatttat ttttactatt gttgatatat tgtaatgttt
     5461 aagccttgag tgtttaccac tcaaggcttt tttagtttta ttgtaacaaa taccctgtac
     5521 agttcacaaa attaaaaaga gagggagatt tatatgagga acaaaaaaat taatattatt
     5581 tttctgacga caatcatgtt tataatgtca acagtaatgg tttttgcaga agaagatata
     5641 gacactattg cactagctaa tgcagaaaaa tttgggattt taactttaat accaccatta
     5701 gttgctataa tacttgcatt tataactaaa aacgtaataa tttctttact tataggtatt
     5761 ctttctggta gttttataat taaagctagt ggtattaatg tttttgcgac atttatacag
     5821 gcatttttag atttagtaga tagagcttta gtatctttag cagacccttg gaatgcaggg
     5881 ataatactac aggttttggc aattggagga gtaataaatc ttgttgctaa aatgggagga
     5941 gctaaagcta tagctgaagc acttgctaaa agagcaaagt cagcaaaagg gacacagctt
     6001 ataacatggt ttttggggct tttagtattc tttgatgatt atgccaattc actaatagta
     6061 ggtcctatga tgagacctgt tgcagataaa atgaaaatat caagggaaaa actagccttt
     6121 ataatagatg ctacagcagc accagttgct ggtttagcta taatatctac atggataggt
     6181 cttgaggttg gtttaataca tgatgctttt gaaagtataa gtatagatgt agatgcattt
     6241 ggtatatttt taaatacaat accatttaga ttttacaata tattaatatt ggcgttcatt
     6301 gtaatatcag cattattatt aaaagaattt ggaccaatga gaaaagctga gataaaatct
     6361 agaagcagaa aaattagtat agaccttgat gaaggcgtag cagagttgga tgatttagct
     6421 ccaaaaaatg gagtaaaatt gagtgtatgg aatgctataa tcccaatagg gacattgata
     6481 atagttgcat tagcatcatt ttattatagt ggatatacat ctataatggg aggagatgat
     6541 aaggctctga tacaactatt tacaaattct ccatattctt ttgaagcaat aaaagaagca
     6601 tttagtgcat ctgatgcatc tagagcatta ttccagtctg cacttgttgc aagtttagtt
     6661 gctattataa tggctgtagt aaaaaagatt tttacaatat cggaagcaat tgatgtttgg
     6721 atagatggaa tgaaaagttt agtaatcact ggtgtaatat taatacttgc atggtcatta
     6781 agttcagtaa taaaagagtt aggtacagct aaatttttaa tacacctatt atcaggttca
     6841 ctaccaccat tcttattacc aagtttaata tttggtttag gagcaataat atcttttgct
     6901 actggaacag cttatggaac aatgggtata cttatgccac ttgcaattcc attagcatac
     6961 tcattaaatc ctgatatgtc ttatgtaata gtaagtacta gtgcagtttt aacaggtgca
     7021 atatttggag accattgttc tccaatttca gatacaacaa tactttcttc aatgggagct
     7081 ggctgtaatc atatagacca tgttaacact caaatgccat atgctatatt tacagcagta
     7141 atcactattg tatttgggta tataccagct ggacttggat tgccaatata tatagtatta
     7201 ccagtggcaa tagctgctat atttataggt attcaaataa ttggtaagag agttgatgaa
     7261 gcggaaatag aacttgtaga ataaagatat aaataagttt caccttttat tatcattatt
     7321 ttgttagttt ttagataatt ttaaaaagtt attgaaaata atagcgaaat atagtaaaat
     7381 aaaagagtga taagcggatg tggcggaatt ggcagacgca ctagacttag gatctagcgc
     7441 cattggcgtg ggggttcgac tcccttcatc cgcactttac agtacttaat tgtactgaaa
     7501 aaaactgggg ttgctggctt tcggagtggg ctatcccgtt tttatttttt tgtatatatg
     7561 atagattggc actttagtat aaagtgcttt ttttataggc aaaaacagta catttatttc
     7621 tgttttatta tcattcatct attagaacat actttagtta agaatgtgtt aataagcagt
     7681 gttcacattc ttgataaaat gtggaataat atgtatcttt actaagacgg aaatgaagta
     7741 attaaactat taaataatat tataagacga taaataagta gataaatcaa attttgtagg
     7801 aggatattat ggaaaaatat atatggttgt ttccattact ttttattttt catgatatgg
     7861 aagagataat aggttttgga atatggctta aaaaaaataa atcaatgctt gataagaagt
     7921 atccatttat cagtaaaatt tatgaaaatt atagtactga aggcatggca tttgctgtgt
     7981 ttgaagaatt tattttatgc attattttct gtatacttat agttataaca gataatcagt
     8041 atgtatattt actatggtta ggtagtttta tcgcatatac tttacatttg gtaattcata
     8101 tagggcagtc aatcataata agaaaatata taccttcact tattaccagt atcatatgcc
     8161 taccaataag catttggtgt ataagtaaga gtatttatat agttgattgt gaaatgagta
     8221 caacaattct gtacagtata attggtatta taattgtagc attgaattta aagtttgcac
     8281 agtcgctgat tggaaaattt acaaagtgga tgtcaaacat ataaatttca gtttattggg
     8341 cagttcaagg agtcagaaat tggagtttta tagtcttaat ataatgtata aaattttata
     8401 gcaatgtatt tcataagtat aaagtgacta gaactttaag aaatatacaa attctataga
     8461 aactaaatag tttcaagtat attttaaaaa tcttattttt cacaagttta aaattgtaaa
     8521 cgatttcatc aagtctatga atatactttt aacatgttat agtaaatctc aatataaatt
     8581 tacatttggt tctttgtgtg ttattttaat aataaattaa gtgaaaatgt tattttacat
     8641 taatatataa tatctttgca taagagtgaa taatataaaa gagaaataat tgataataaa
     8701 aacttaacaa aaaaattaat acataaaaaa aaataaatat aatgtgttga catttaattg
     8761 aaatattcat acaataaaat tgaaaataaa tatttccaaa tgagattagc gagagagtcc
     8821 tttatacgaa aaggcgccga agaagaaatt taagaaatgc caatttttag agaagctttc
     8881 aggcaaaaat atcgctaatt gatgggactc tggagagact taagttaaga tactataagt
     8941 caccgaagaa gcaaaataat attaaattat taaatctttc aggtatacaa acagggatat
     9001 ataggctagg attattttcc atgctgatat atcttttttt tatgaaaaag tacaggaaat
     9061 aatatcaaaa ttacttaaat acagtaggtc taattttata gggagggtta tatatgaatg
     9121 aattgaaaag agtatctttg tacaatatac acaaagagct tggagcaaaa cttgttgaat
     9181 ttgctggatg ggaaatgcct ttagaatatg aaggtataaa taaagagcat gagatggtca
     9241 gaaaaagtgc aggtatattt gatgtatctc atatgggaga ggtacagata aagggagtag
     9301 agtcagaaaa atttatacaa aacctagtta caaatgatat aagcacatta aatataaatg
     9361 acattatata tacacctatg tgttatgaaa atggtggagt agttgatgat ttattaatat
     9421 ataaatttgg tgaagaagag tatttattag taataaatgc aggtaatata gataaagatg
     9481 tagcatggat aataaaacaa agtgaaggat acaatgtaga tataaaaaat atatctagtg
     9541 aagtgagtca attagcaatt caaggaccta aagcagaaga aattcttcaa aaaatcacag
     9601 atatagactt aaactcaata aaattttata agtcaattcc atcaacaaaa gtatgtggtt
     9661 gtccttgttt agtttcaaga acaggatata ctggggaaga tgggtttgag atatactgta
     9721 aaaataaata tgtagaaata atttggaatg aagttctaaa ggttggagga gaagatatat
     9781 gtccagctgg tctaggttgt agggatactt tgagatttga agcagctcta cctctatatg
     9841 gacatgaaat aaatgaacat atatctccaa ttgaaggtgg cttatctatc tttgtgaaaa
     9901 caaataaaga atcatttatt ggcaaatcaa ttttatcaaa agaaaaagaa agtggagcta
     9961 aaagaaagtt agtaggattt gaaatgcaag gcaaaggaat gcctagaaat ggatatgata
    10021 taaggattgg tgataaaata gttggatttg taacaacagg atgtgcatct ccaacaacag
    10081 gaaaaatttt aggtatggga attatagatt ctgagtatgc aaaagtagga aatgaaatag
    10141 gtatagcaat cagaaaaaaa gttgttcctg ctgtgatagt taagaaacct ttttataaaa
    10201 aacaatataa aaaagataat attatattaa acaaagaaaa taaattttca tatatacctg
    10261 ctacaagtga agataaatca aaaatgttaa aagtagttgg attaaattcg gttgatgaat
    10321 tattttcaga tataccagaa gaagtaaaat taaaaagaga tttaaatttg gaaattggaa
    10381 aatctgaatt agaggtaagc aaaatagtaa aaagattgtc agaagaaaat ttaagtttag
    10441 aagacctgac ttgttttctt ggtgctggtg catatgacca ttatatacca tcaattataa
    10501 aacatataac ttcaaggtct gaattttata cagcatacac tccatatcaa gctgaaataa
    10561 gtcagggaac tttacaagta gtttttgaat ttcaatctat gattgcagaa attacaggta
    10621 tggaaattgc aaatgcatca atgtatgatg gagcaactgc tgcaatagaa gcatgtataa
    10681 tggcaataaa tcagactaga aaaagtaaaa tagtagtatc aaaaactact catcctgaaa
    10741 ctttatcagt actaagaact tacttacaat ataaagattg tgagatagta gagattgact
    10801 tttgtaatga atatgggact acagatatag aaaaattaaa agcttctgta gacaaagata
    10861 cagcatgtgt tcttatacaa actcctaatt tctttggaat tattgaagaa atggaagaaa
    10921 tagaaaagat aacccatgaa aataaggcta tgttaattat gagtgtagac ccaatatcat
    10981 taggagtttt aaaaacacca ggtgagatag gtgcagatat tgttgttgga gaagctcaat
    11041 cacttggaaa ccctcttaat tttggaggtc cttatgtagg atttttagct agcaaatcta
    11101 aatatactag aaaaatgcca ggaagaatag ttgggcaaag tttagatgta gaaggtaaaa
    11161 ttgcatatgt attaacttta caaactagag aacagcacgt aagaagagaa aaagcaactt
    11221 ctaatatatg ttcaaatcaa gccttaaatg ctcttgtagc ttcaatatat atggctacta
    11281 tgggtaaaga aggatttaaa gaggttggta tgcagtctat gaaaaaagca cattatactt
    11341 ataataagct tgtacaaact ggaaaatata agccaatatt taaaggtaag tttttcaaag
    11401 aatttgctgt acaaggaaat cttaatatag aaactataaa tgataaactc ttagaagaaa
    11461 atatattagg tggttataat ttagaataca attatccaga attaaaaaat tcaactttgc
    11521 tttgtgtgac tgagaaaaga agcaaagagg aaatagataa gttagttgga ataatggagg
    11581 gattataatg aaagaataca atagtttgtt gatagatata tctaaaaaag gtagaaaagc
    11641 atactcactt ccaaagttag atatagatga tataaaaata gaagatatga ttgaccaaga
    11701 tatggcaaga caaagtgagt tgaatttgcc agaagttggt gaattagaat tagttagaca
    11761 ttatacatta ttatcaaata aaaactttgg agttgacaca ggtttttatc ctttgggttc
    11821 ttgtactatg aaatataatc ctaaaataaa tgaagatatg gcagcacttc caaactttac
    11881 tggaatgcat ccgtaccaat cttcggatac agctcaaggt tctctttctt tgatgtatga
    11941 tttatcaaga agacttgcag aaattacagg tatggatgaa gttacattac aaccttcagc
    12001 aggttcgcat ggtgagttta cagggcttat gattataaaa gcatatcatg aaaatagagg
    12061 tgaccataag agaaccaaag tgataattcc agattctgca catggaacaa atccagcaag
    12121 tgcagctatg gcaaactttg atgtgattca aatagcatct gataaaaatg gagctgtaga
    12181 cataaatgca ttaaaagagg ttttaaatga tgaagtagcg gctcttatgc ttactaatcc
    12241 aagtactctt ggtttatttg aaaaaaatat aaaagaaata gctactcttg tacatgaagc
    12301 aggaggactt ttgtactatg atggtgcaaa catgaatgct attatgggga taacaagacc
    12361 tggagatatg gggtttgatg tggttcatct taatcttcat aagacttttt caactcctca
    12421 tggaggtgga ggtccaggag ctggtcctgt tggagttaaa aaagagttag tacctttttt
    12481 accaatacca ataattgaaa gaaaagaaga taagtatgta ctaaattatg atagagaaaa
    12541 atctattggg aaaattaaaa atttctatgg taattttgga gttcttgtaa gagcttacac
    12601 atatatacta actatgggaa gggatgggct taaagaggct agtgaaatgg cagttttaaa
    12661 tgctaattat ataaaggaaa gtataaaaga tgattatatt ttaccaatag ataccttgtg
    12721 taagcatgag tttgtattag gtggattgcc aagaggagag gccaatataa aaactatcga
    12781 tattgcaaaa agactactag attatggata ccatcctcca acaatgtact ttcctcttat
    12841 tataaatgag gcacttatga tagaacctac agaaagtgag agtatagaga cattagatag
    12901 ttttatagaa gctatgaaaa gtgttgctaa agaggcaaag gaggaaccag aattattgaa
    12961 aactgctcca cataatactt tggtcaaaag agtagatgac gcaagggcag taaagaaacc
    13021 tatactaaca tggtcacaaa gataaataat taaaaagtag agatttaaag tataaagaga
    13081 attaaattta taagaaaaag ctatctaact taatctagtt tagatagctt ttttgtatat
    13141 tagtatgtat ggtattttct atataaagtt ttaaattata ttagtgctta tatagaatat
    13201 tgatgtgatg attatgtata gatattcata agaattgttg aatttatct
//
LOCUS       DAEPXF010000101        13033 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain HB27
            SAMN13376389-rid12479423.denovo.107, whole genome shotgun sequence.
ACCESSION   DAEPXF010000101 DAEPXF010000000
VERSION     DAEPXF010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13376389
            Sequence Read Archive: SRR10518432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 13033)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13033)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001069624.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001069624.1
            Genome Coverage       :: 315x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/15/2021 01:54:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,165
            CDSs (total)                      :: 4,076
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 89
            rRNAs                             :: 10, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 10 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 19 of 69
            Pseudo Genes (incomplete)         :: 44 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 10 of 69
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13033
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13376389-rid12479423.denovo.107"
                     /strain="HB27"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="China: Hebei"
                     /collection_date="2017"
     gene            complement(<1..1174)
                     /locus_tag="KRL67_002507"
     CDS             complement(<1..1174)
                     /locus_tag="KRL67_002507"
                     /EC_number="3.5.4.25"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000469013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3,4-dihydroxy-2-butanone-4-phosphate synthase/GTP
                     cyclohydrolase II"
                     /protein_id="HBH1523376.1"
                     /translation="MFNTIEEAIEDIKNGKMVIVVDDESRENEGDLLMAAEMATPESI
                     NFMATYGRGLICLPATEKKFKSLNIPLMVRENTDTFQTAFTVTIDGADTLTGISAYER
                     AETVKLFCDENSTSKDFKTPGHIFPLIAKTGGVLVRDGHTEASVDLARLAGFKEIGLI
                     CEIMKDDGTMARVDDLMIFKEKHNLKIITIKDLIEYRKINETTIEKVSSAFLPTKYGN
                     FEIIGYRDTYSNEEHIALTYGNINVENTLVRLHSECLTGDVFHSLKCDCGLQLESSMK
                     KIVENGSGVLIYMKQEGRGIGLLNKIKAYKLQEEGYDTVEANLMLGFEEDMRDFYMAA
                     QILKNLNIKSINLLSNNPDKINQLEKYGIKIENRIPINEEINDFNKLYLKTKKDKMG"
     gene            complement(1197..1862)
                     /locus_tag="KRL67_002508"
     CDS             complement(1197..1862)
                     /locus_tag="KRL67_002508"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HBH1523377.1"
                     /translation="MFTGIVEEVGILRKITANGKSGKVTILSNKILDGTNLGDSIAVN
                     GVCLTVSNLGKNEFTADVMMETIRSTNLGLLNANDKVNLERAMSLSSRFGGHIVTGHV
                     DGKGTICKFEKDENAVLVSIRPDKKLLSSMILKGSVAIDGVSLTISYLDDEIFKVSII
                     PHTKSNTILLTKNVGDFVNLESDVIGKYVNNFMVNNYKELNSNSSNHKSNIDKDFLFK
                     NGF"
     gene            complement(1896..3008)
                     /gene="ribD"
                     /locus_tag="KRL67_002509"
     CDS             complement(1896..3008)
                     /gene="ribD"
                     /locus_tag="KRL67_002509"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HBH1523378.1"
                     /translation="MSFVNQKEKDIYYMKKAIELAKNGEGFVNPNPLVGCIIVKDSNV
                     IGKGYHEKFGSNHAEVNAINSAKQSLKDSTLYVNLEPCSHYGKTPPCVDKIIQNKIKR
                     VVISTLDPNPLVCGNGVKKLKDNNIDVTVGILEEEARDLNEAFFYYIKNKKPLCIVKS
                     AVSLDGKIATKSLESKWISNESSRYLTHKYRNKYQSIMVGINTVLNDNPLLTCRLNQE
                     KVSHPTRIVIDTHLKLPLNSNLVKDKTSKTIVFTCCKESKKLSMLKENNVETIISPSK
                     NNLVDLEFVMYKLGELNIDSVLVEGGATLNDSLFRNKLVDKVKLFLSPKIIGGKDAPT
                     FVSGEGINHLSDSTQLTINNVTLIYGDILIESDVLN"
     regulatory      complement(3140..3261)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            3450..5933
                     /gene="recQ"
                     /locus_tag="KRL67_002510"
     CDS             3450..5933
                     /gene="recQ"
                     /locus_tag="KRL67_002510"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HBH1523379.1"
                     /translation="MNKNLLEILSKYYGYTSFRKGQESIINSILSEKDVLAIMPTGGG
                     KSICYQIPALILDGMTIVISPLISLMKDQVDALKTMGISAAFINSSLSSKEFNEILNN
                     IRKNNYKILYIAPERLDAQEFLELINNNNVSQVAIDEAHCVSQWGHDFRLSYRRISDF
                     IKNLPKRPIVTAFTATASEEVRTDIINLLCLENPDYYITGFDRENLSINIVKSSSKNK
                     YILDYIQNHKNESGIIYVATRKEVENIYNGLSKRNISVSKYHAGLSKNERSKNQEDFI
                     NDNVDIMVATNAFGMGIDKPNIRWVIHYNMPQSIENYYQEIGRAGRDGEKSECVLLFS
                     PGDVHIQKYLIDIGVENPERKLFQHKKLQYMIDLVYSNSCYRKTILSYFGENYIDNCN
                     NCSNCLVEGEIVDKTIDAQKVISCVARMKRSYGVTTIVDVLRGSKNKKILQLGLNTLS
                     TYNIMRDYSSEDLKNFINTLVSHGFLDLVETLGNGNRGSFPTIRLNEMSMKVLKGEVK
                     VEFKEIVMTKSLEVEDKLYNALRELRHSIASEERIAPYMVFGDGTLRAMSSSYPINKE
                     EMLDISGVGEIKYQKYGRDFEAVIKEYVEKNNINKKIDEKNVDLKNINSDFLEVNTDK
                     VLYEKLLVIREEYATKEKLPAYMVLANKALKEISGRYPLDEEQLKDISGIGGVKFEKY
                     GASILAEVNKYVKENNINVIWEKKGKRKLILDGESRKNNEIALDLLNQGKDIQSISQE
                     IEVSLSTIIGYVHDYVKDGHSISFELGLENFYNEDKKKMILEVCEKVGYDNLNNIKRK
                     LPDSIKYENIRAVILDSYLENLKNNIIAE"
     regulatory      6022..6133
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            6275..7576
                     /gene="thiC"
                     /locus_tag="KRL67_002511"
     CDS             6275..7576
                     /gene="thiC"
                     /locus_tag="KRL67_002511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HBH1523380.1"
                     /translation="MNYTTQMDAARKGIITKEMEIVSQKEQVDVNELRELIANGQVVI
                     PANKNHKSLSAEGVGKNLRTKINVNLGISRDCKDIEKELEKVRVAIDMKAEAIMDLSN
                     YGKTREFREKVVEMSPAMIGSVPMYDAVGYLEKELKDITEEEFLNVIRQHAIDGVDFI
                     TIHAGLTRSVCQKIKNHERLTHIVSRGGSLLFAWMELNNKENPIYTNFDKILDICEEY
                     DVTLSLGDACRPGCIKDSTDGVQIQELVVLGELTKRAWERNVQVMIEGPGHMAIDEIE
                     ANVVLEKRLCHGAPFYVLGPLVTDIAPGYDHITSAIGGALACAKGVDFLCYVTPAEHL
                     RLPNLDDMKEGIIAAKIAAHAGDIAKNVKGAREWDNKMSKARADLDWCEMFRLAIDPE
                     KAKRYRDESTPTHEDSCTMCGKMCSMRTVKKILNNEELNLI"
     gene            7630..7824
                     /gene="thiS"
                     /locus_tag="KRL67_002512"
     CDS             7630..7824
                     /gene="thiS"
                     /locus_tag="KRL67_002512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HBH1523381.1"
                     /translation="MKVNGKEIEFEKDLTVIDLLNKYNLKSDRVVVEVNLEIIEESNY
                     NTYVLKDEDIVELISFIGGG"
     gene            7817..8620
                     /gene="thiF"
                     /locus_tag="KRL67_002513"
     CDS             7817..8620
                     /gene="thiF"
                     /locus_tag="KRL67_002513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine biosynthesis protein ThiF"
                     /protein_id="HBH1523382.1"
                     /translation="MVKIKIFVNESLTDVEENTTAYKVRDSYNNKCDVLVLNGYPIKT
                     DMPLCENDKLTLIQKGVKPSLDELENLLIARHTPNIHNKLKKGKVAILGLGGLGSNVA
                     ISLARIGVGELLLIDYDVVEPSNLNRQQYFIDDIGKLKTDAMIENIKKINPFIKLNKR
                     DIFLNKNNMDIIKDSDLIIEAFDDASCKAQVCNYVLINLKDKYLIASSGMAGYYDSNI
                     ITTKKIKDRFYICGDFVNEAKFGEGLMAPRVAICANHMANLATQILIDM"
     gene            8682..9452
                     /locus_tag="KRL67_002514"
     CDS             8682..9452
                     /locus_tag="KRL67_002514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018592874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HBH1523383.1"
                     /translation="MDKLVLGGHEFSSRLLVGTGKYGSNNILPEVIKESGSEIITMAL
                     RRVDLDNKQENILTYIPKEMTILPNTSGATNAKEAVRIARISRKMGCGDFIKIEVISD
                     TRYLLPDNEETIKATKILADEGFIVLPYMTPDLYAGRRLIEANAAAVMPLGAPIGSNR
                     GLQMKEMIRIMIDELDIPIIVDAGIGKPSQAMEAMEMGADAVLVNTAIASAGDPVQMA
                     RAFKLAVEGGREAYIAKTGNVSEFANASSPLTGFLGNL"
     gene            9469..10572
                     /gene="thiH"
                     /locus_tag="KRL67_002515"
     CDS             9469..10572
                     /gene="thiH"
                     /locus_tag="KRL67_002515"
                     /EC_number="4.1.99.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminoacetate synthase ThiH"
                     /protein_id="HBH1523384.1"
                     /translation="MSFYDVIEKYRDFDFDGYLNNVTDNDVLRSLSKDKLEDLDILNL
                     LSKTAVKHLEDMAQKAHKLSVQYFGKTVCLYTPMYIANYCVNQCVYCSYNIKSGIKRK
                     KLTMDEIREEGEAISKEGFKHLLVLTGESSFHSSVEYIGEAIEILREKFPSIGIEVYP
                     MEVEEYKYIVDKGVEGLTVYQETYDEEIYKRVHIKGPKSNYKFRLDAPERGAKAGMRT
                     LSIGALLGLNDFRKETFFTILHGKYLKTKYPHIELSYSTPRMRPFKGCFEELVDISDT
                     DLVQAMVCMRLFDPHAAINISTRENLEMRSHIIPLGVTKLSAGVSTDVGGHSQDEHDT
                     AQFKINDESTVKDVEKMLNSIGYQHVFKDWERF"
     gene            10573..11262
                     /locus_tag="KRL67_002516"
     CDS             10573..11262
                     /locus_tag="KRL67_002516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HBH1523385.1"
                     /translation="MYLITNRKLCSEERYLEVIKESILSGVENIIIREKDLEYQELKK
                     LYMKIKTKINCIDFQEQISDESLKTNINQKECRNKFKVNFIINSNIEFFEKVDCQGIH
                     LPFKLFLNLIENKYNFNENKILGLSLHKVEEVDYLEKLIRNQNIKIDYITLSHIYETK
                     CKEGLNPKGIELLKEAKKITDIKIIALGGILPSNVKETLKYCDDFAIMSTIMKSKDIK
                     KTISNYNEKLN"
     gene            11450..12844
                     /gene="yfcC"
                     /locus_tag="KRL67_002517"
     CDS             11450..12844
                     /gene="yfcC"
                     /locus_tag="KRL67_002517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HBH1523386.1"
                     /translation="MTDSKKKRFIIPHTFTIIFLLIVLMAILTWIVPSGEFSREDVEG
                     RMVVVPGTYQSVESNPQGFADVFKAPIEGFIDSAEVVGFVLIVGGAFGIVNRTGAIEA
                     GIASVISKFKGKELLIIPISMILFGLGGTTFGMAEETLPFYMIFVPLMTSLGYDSLTA
                     IAIVFIGAAAGAAASTINPFSVGIAQALAQLAPGSGVTYRVVIFIAYILISIAFVMYY
                     AKKVKENPESSIVYEIDKNNKSILNQTSTEIKEFTFKEVSVLAIFGIGMAIMIWGVLS
                     QGWYIPEISMCFAAIGVLSGLVGRLSQIEISESFIDGAKDLASAALAIALARGIVIIA
                     QNGFIIDTILNSAAAFLGGLPKVIFVYLSFFLEAALAFLIPSSSGLASLTVPVLAPLG
                     DLVNISAQVIVTAYQFGVGLTNFITPTSGVLMGALAVAHIPFSKWVRFIMPILLILTA
                     VSLVFLTIGIYIGF"
BASE COUNT         4891 a         1568 c         2211 g         4363 t
ORIGIN      
        1 gtcccatttt atctttttta gtttttaaat atagcttatt aaagtcatta atttcttcat
       61 taatagggat tctattttcg atttttattc catatttctc taactgattt attttatcag
      121 ggttattact taaaagattt atacttttta tgtttaaatt ttttaatatt tgagcagcca
      181 tatagaaatc tctcatatct tcttcaaatc caagcattaa atttgcttca acagtatcat
      241 aaccttcttc ttgaagttta taagccttaa ttttatttaa aagaccaata cctctacctt
      301 cttgcttcat ataaattaaa actccacttc cattttccac aattttcttc atagaagatt
      361 ctagctgtaa tccacaatca cattttagag agtggaacac atctcctgtt aaacattcag
      421 aatgaagtct aactagagta ttttctacat ttatatttcc ataagtaagg gcaatgtgtt
      481 cttcatttga ataagtatct ctatatccta tgatttcaaa gtttccatat tttgttggaa
      541 ggaaagcact agatactttt tctatagtag tttcatttat ttttcggtat tctataaggt
      601 cttttatagt gattattttt aaattatgct tttctttgaa aatcattaaa tcatcaactc
      661 ttgccattgt accatcatct ttcattattt cacagataag accaatctct ttaaaacctg
      721 caagtctagc aaggtctact gaagcttctg tatgtccatc cctgacaaga actccaccag
      781 tttttgctat taaaggaaag atgtgaccag gtgttttgaa atctttactt gtagaatttt
      841 catcacagaa taattttaca gtttcagctc tttcatatgc agatatacca gttagagtat
      901 ctgccccatc tatagtaaca gtaaaggctg tttgaaatgt atctgtattt tctctaacca
      961 taagaggtat attaagactt ttaaattttt tttcagtggc aggtagacaa attagacctc
     1021 taccatatgt agccataaaa tttattgatt caggagtagc catttcagct gccatcaata
     1081 aatctccttc attctctcta ctttcatcat caaccacgat taccattttt ccatttttta
     1141 tatcttcaat agcttcttca atagtattaa acatgtaatt cactccttta ttatttttaa
     1201 aacccatttt taaataaaaa atctttatct atatttgatt tatggttact actattagaa
     1261 tttaattcct tgtagttatt aaccataaaa ttattaacat actttccaat tacatcactt
     1321 tctaaattaa cgaaatctcc aacattcttt gtcaagagaa tagtgttact tttagtatga
     1381 ggtataatag aaaccttaaa tatttcatca tctaaatatg atatagttaa acttacacca
     1441 tcaatagcaa cagaaccttt gagtatcatt gatgataaaa gttttttatc aggtcgtatg
     1501 cttactaaaa ctgcattttc atctttttca aatttacaga tagttccttt accatcaaca
     1561 tgacctgtta ctatatgacc accaaatctt gaagaaagac tcatagctct ttcaagattt
     1621 actttgtcat ttgcatttaa taaacctaaa tttgtggaac gaattgtctc catcataaca
     1681 tcggctgtaa attcattttt tccgaggttt gaaacagtta gacaaactcc atttacggct
     1741 atactatctc caagatttgt gccatctaat attttatttg atagtatggt tacttttcca
     1801 ctcttaccat tagcagtaat ttttctaagt attccaactt cttctactat gccagtaaac
     1861 atcctaacct cctatatgca tgtattaaca aaaatttaat ttaaaacatc tgattctata
     1921 agaatatccc catatatcaa agttacatta tttattgtta actgtgttga atctgacaaa
     1981 tgatttattc cttctccaga aacgaatgta ggtgcatctt taccacctat aatcttgggt
     2041 gatagaaata atttgacttt atcaactaac ttatttctaa agagactgtc attgagagta
     2101 gcaccacctt caactagaac agaatcaatg tttaactctc ctaatttata cattacaaat
     2161 tccaaatcta ctaagttatt ttttgaagga gaaattattg tttcaacatt attttctttt
     2221 aacatagata attttttact ttccttgcaa catgtgaaaa ctatagtttt actggtttta
     2281 tctttaacca aattactatt aagtggcaac tttaggtgtg tatctattac tattcttgtg
     2341 ggatgagaca ctttttcttg attgagccta caagttagta aaggattatc gttcaaaact
     2401 gtatttatgc caaccattat actttggtat ttatttctgt atttatgagt tagatacctt
     2461 gatgattcat tagatatcca ttttgattct agtgattttg tagcaatttt accatctaga
     2521 cttactgctg attttacaat acaaagaggt tttttgtttt ttatataata aaaaaatgcc
     2581 tcatttaaat ctcttgcttc ctcttctaat attcccacag taacatcaat attgttatct
     2641 tttaattttt ttactccatt accacaaaca agtggatttg ggtctaatgt agatattaca
     2701 actctcttaa tcttattttg tataatttta tctacacaag gaggcgtttt tccataatga
     2761 gaacatggtt ctaagtttac ataaagagta gagtctttta aactttgttt agcactgttt
     2821 atagcattta cttcagcatg attagagcca aatttctcat gatatccttt gcctattaca
     2881 ttactatctt taacaattat acatccaact aatggatttg gatttacaaa gccttctcca
     2941 tttttagcca attcaatagc ttttttcata taataaatat ctttttcttt ttgattcaca
     3001 aaactcacaa tatgtacacc tcctgtttaa ttggataatt tttaaacaaa aaaccctgag
     3061 aattaactca gggtttagaa taatctacaa taaatgttta ataagtgtag aacaaaattg
     3121 ctctacaaaa acactcatta aatatcttct tccatccaga ctttactgtc ggctttggaa
     3181 tttaaccaaa tcaaccaagt tttaccttgg ctcgcgggct ataccgccga ttgggaattt
     3241 caccctaccc cgaagattaa gtattaaatt tatatttaga atgtatcata aattatttta
     3301 ctttgcaaac tattttttta aaaaatatat gttaatatat aatatagaat gtctataaac
     3361 tttgaaatac cacgattgtg aatataaaaa taaaaatttg aacataatca ggaaagaagt
     3421 ctaattaatt taaattagga gagtaatata tgaataaaaa tcttttagaa atactatcaa
     3481 aatactatgg atatacaagt tttagaaaag gtcaggaatc tataataaat tctatcttaa
     3541 gcgagaaaga tgtgttagct attatgccaa caggaggggg aaaatcaata tgttatcaaa
     3601 ttccagcttt gattttagat ggaatgacaa tagtaatttc tccacttata tcacttatga
     3661 aagaccaagt agatgcttta aagactatgg gaattagtgc tgcttttata aatagttcat
     3721 taagtagcaa agaatttaat gaaatattaa ataatattag aaaaaataat tataaaatac
     3781 tctatattgc tccagagaga ttagatgcac aagagtttct tgagctaata aataataaca
     3841 atgtaagtca agttgcgata gatgaagcac attgtgtatc tcaatggggg catgatttta
     3901 ggttgagtta tagaaggatt tctgatttta taaaaaattt gcctaaaaga cctatagtta
     3961 cagcatttac tgcaacagct tcagaagagg taagaactga cataataaat ttactgtgtt
     4021 tagaaaatcc agattattat ataactggat ttgatagaga gaatttatca ataaatatag
     4081 taaaatcatc tagtaaaaat aagtatattt tagattatat tcaaaatcat aaaaatgagt
     4141 ctggaattat atatgttgcg actagaaagg aagttgaaaa tatatataat ggtttatcta
     4201 agagaaatat atctgtgtca aaatatcatg caggtttatc taaaaatgaa agaagtaaaa
     4261 atcaagaaga ttttataaat gataatgtgg atataatggt agcaacaaat gcatttggaa
     4321 tgggtattga taaacctaat ataagatggg ttatacatta caatatgcct caaagtatag
     4381 aaaattacta tcaagaaata ggtagagctg gtagggatgg agaaaaaagt gagtgtgtac
     4441 ttttattttc tccaggagat gtacatatac aaaaatattt aattgatatt ggagttgaaa
     4501 atcctgaaag aaaattattt cagcataaga agcttcaata catgattgac ttagtttaca
     4561 gtaattcatg ctatagaaag acaatattga gttattttgg agaaaattat atagacaatt
     4621 gcaataattg tagcaattgt ttagtagagg gtgaaatagt agataaaact atagatgcac
     4681 aaaaagtcat ttcatgtgta gctagaatga aaagaagtta tggtgttact actattgtag
     4741 atgtacttag aggttctaag aataaaaaga ttttacagct tggattaaat actttgtcta
     4801 catataacat tatgagagat tattctagtg aagacttaaa gaattttata aatacattgg
     4861 tttctcatgg ttttttagat ttagttgaga ctctaggcaa tggaaataga ggtagttttc
     4921 ctactataag attaaatgag atgtctatga aagttttaaa aggtgaagtg aaagtagagt
     4981 ttaaagaaat tgttatgaca aaatcattgg aagtagagga taagttatat aatgctttaa
     5041 gagaacttag acattcaatt gcaagtgaag aaaggatagc tccatatatg gtatttggag
     5101 atggtacact tagagccatg tctagtagtt atccaattaa taaagaagaa atgctagata
     5161 tctctggagt aggagaaatt aaatatcaaa aatatggtag agattttgaa gcagtaatta
     5221 aagaatatgt cgaaaaaaac aatataaata aaaaaataga tgaaaaaaat gtcgatttaa
     5281 agaacatcaa tagtgatttc ttagaggtta atacagataa agttttatat gaaaaactgt
     5341 tagtcattag agaagaatat gcaacaaagg aaaaattacc tgcatatatg gttttagcca
     5401 ataaggcttt gaaagaaata agtggaagat atccacttga tgaagaacaa ttaaaagata
     5461 tttctggcat aggtggagta aaatttgaaa aatatggagc tagtattttg gcagaggtta
     5521 ataaatatgt aaaagaaaat aatattaatg ttatttggga gaaaaagggc aaaaggaagt
     5581 taatattaga tggagaaagt agaaaaaata atgaaatagc attagattta ctaaatcaag
     5641 gaaaagatat acaaagtatc agtcaagaaa tagaagtttc actgtcaacc attattggat
     5701 atgtgcatga ctatgtaaag gatggacata gtatatcatt tgaattagga ctagaaaatt
     5761 tttataatga agataaaaag aagatgattt tagaagtttg cgaaaaagtt ggctatgata
     5821 atttaaataa cataaaaaga aaacttccag attcaataaa gtatgaaaat ataagagctg
     5881 tgatacttga ctcttatctt gaaaacttaa aaaataatat tatagcagag taatttttaa
     5941 cttaataaaa aataaaattt gtattattgt gttgaactaa taattttgct gtgttatact
     6001 gaatttgtaa aaataatgaa aaattagagc taggggcgct agaattggct agctgagaga
     6061 taaagccgta acttacaaac cctaataacc tgatcaagat aatgcttgcg aagggaagct
     6121 ttttaatata attttattat ataaataggc aaattattta atgttagggg aaatttgtct
     6181 aagtgtatat acgattataa gagtgctttc caaacgagga aaagcacttt ttttattttc
     6241 aaaaactata actttaatct tggaggattg taatatgaac tatacaacac aaatggatgc
     6301 tgctagaaaa ggcataataa ctaaagaaat ggaaatcgtt tctcaaaaag aacaggtaga
     6361 tgtaaatgaa ttaagagaat taatagcaaa tggacaagtt gttataccag ccaataaaaa
     6421 tcataaatct ttaagtgcag aaggtgtagg taaaaattta agaacaaaaa taaatgtaaa
     6481 tctaggaatt tcaagagatt gtaaggatat agaaaaagaa cttgaaaaag ttagagtagc
     6541 aatagacatg aaagctgaag ctataatgga tttaagtaat tatggtaaaa ctagagaatt
     6601 tagagaaaag gttgttgaaa tgtcacctgc aatgataggt tcagtaccta tgtatgatgc
     6661 agtaggatat ttggaaaaag aattaaaaga tataacagaa gaagagtttt taaatgtaat
     6721 aagacaacat gctattgatg gagtagactt tattactata catgcgggtc ttacaagaag
     6781 tgtttgccaa aaaataaaaa atcatgagag attaactcat atagtatctc gtggaggttc
     6841 tttattgttt gcatggatgg aattaaataa taaggaaaac cctatttata ctaattttga
     6901 taaaatattg gatatatgtg aagaatatga tgttacattg agtttaggag atgcttgtag
     6961 accaggttgt ataaaggatt ccactgacgg tgttcaaatt caagaacttg tagtattagg
     7021 tgaacttact aaaagagcat gggaaagaaa tgtacaagta atgatagaag gtcctggaca
     7081 catggccatt gatgaaatag aagcaaatgt agtattagaa aagagattat gccatggagc
     7141 acctttttat gttcttggac cattggttac agatatagca ccaggttatg accatataac
     7201 aagtgcaata ggaggagctt tagcatgtgc taaaggagtg gattttttat gttatgtaac
     7261 tccagcagaa catttaagat tgcctaattt agatgatatg aaagaaggta taatagctgc
     7321 aaagatagct gctcatgcag gagatatagc taaaaatgtt aaaggtgctc gtgaatggga
     7381 taataagatg agtaaagcta gagctgattt agactggtgt gagatgttta ggcttgcaat
     7441 agaccctgaa aaggctaaga gatacagaga tgaatcaact cctactcatg aagatagttg
     7501 tactatgtgt ggaaaaatgt gctcaatgag aacggttaag aaaatattaa acaatgagga
     7561 acttaatcta atataatttt tggctaatat gactggaata ataagaagct gaaaagagga
     7621 ggtgatttag tgaaggttaa tgggaaggaa attgaatttg aaaaagattt gactgttata
     7681 gatttattaa ataaatataa tttaaaaagt gatagagttg tagttgaagt gaatttagag
     7741 ataattgaag aatctaacta caatacatat gtattaaaag acgaggatat tgtagagctt
     7801 attagcttta ttggaggtgg ttaaaataaa aatatttgta aatgaatcat taacagatgt
     7861 tgaggaaaat acaactgctt ataaagttag agatagctat aacaataaat gtgatgtatt
     7921 ggtgttaaat ggatatccaa ttaaaacaga tatgcccctt tgtgaaaatg ataaattaac
     7981 acttatacaa aaaggagtta agccatcact tgatgaattg gaaaacttgt tgattgctag
     8041 acatactcca aatatacata ataaattaaa aaaagggaaa gttgctatat tgggactggg
     8101 gggacttggt tccaatgtag ctatctcttt agctagaata ggagtgggag aacttttact
     8161 tatagattat gatgttgtag aaccatctaa tttaaataga cagcagtatt ttatagatga
     8221 cataggaaaa ctaaaaactg atgcaatgat agaaaatata aaaaaaataa atccttttat
     8281 aaaattaaat aaacgagata tttttttaaa caagaacaat atggatatta ttaaggattc
     8341 agatttaata attgaggctt ttgatgatgc ttcatgtaaa gcacaggtat gtaattatgt
     8401 attaataaat ttaaaagata aatacttaat agcatcatct ggtatggcag gttattatga
     8461 ttcaaatata ataactacta agaagataaa agacaggttt tatatatgtg gggattttgt
     8521 gaatgaagct aaatttggtg aagggcttat ggctccaagg gttgctatct gcgcaaatca
     8581 tatggcaaac ttggcaacac aaatacttat agatatgtaa atattaaaat tagatattaa
     8641 aaagattgaa tattaaaaaa attaacatgg agggaaataa tatggataaa ttggtacttg
     8701 gtggacatga atttagtagc agattattgg ttggaacagg taaatatggt tcaaataata
     8761 tattgcctga ggtaataaaa gaaagtggaa gtgaaataat aacaatggca cttagaagag
     8821 tggatttaga caataaacaa gaaaacatat taacgtatat tccaaaagaa atgacaatac
     8881 ttccaaatac atcaggagct actaatgcaa aagaagctgt aagaatagct agaatatcta
     8941 gaaaaatggg atgtggagat tttataaaaa tagaagtaat atcagatact agatatctac
     9001 ttcctgataa tgaagaaact ataaaagcta caaagatact tgctgatgag gggtttatag
     9061 ttcttccata tatgacacca gacctttatg ctggaagaag acttatagaa gctaatgcag
     9121 cggcagttat gccacttgga gctccaattg gttcaaatag aggccttcaa atgaaagaaa
     9181 tgataagaat aatgatagat gagttggata tcccaataat agtagatgca ggtattggaa
     9241 aaccatctca agctatggag gctatggaaa tgggagctga tgcagtactt gtcaacactg
     9301 ctatagcttc agcaggagac cctgtacaaa tggctagagc atttaaatta gctgtagaag
     9361 gtggaagaga agcatatatc gcaaaaacag gaaatgtgtc agaatttgca aatgcatctt
     9421 ctcctttaac aggtttttta ggcaatttat agaaagtggt gattggtgat gagtttttat
     9481 gatgtaatag aaaaatatag ggattttgat tttgatggat atttaaataa tgttactgat
     9541 aatgatgttt taagaagttt atcaaaagac aagcttgagg atttggatat attaaatttg
     9601 ctttctaaaa cggcagtaaa acatttagaa gatatggcac aaaaagcaca taaattaagt
     9661 gttcaatact ttggtaaaac agtatgttta tatacaccaa tgtatatagc aaattattgt
     9721 gttaatcaat gtgtttattg tagctataat ataaagagtg gaataaaaag aaaaaaatta
     9781 actatggatg aaattagaga agagggagaa gcaatttcaa aagaaggttt caaacatcta
     9841 cttgttttga ctggagaaag tagttttcac tcaagtgtag aatatatagg tgaggctata
     9901 gaaatattaa gagagaaatt tccctctata ggtatagaag tttatccaat ggaagtagaa
     9961 gaatataagt acattgtaga taaaggtgtt gaaggattaa cagtatatca agaaacttat
    10021 gacgaggaaa tatataagag agtacatata aaaggcccaa aatcaaatta taaatttaga
    10081 ctagatgctc ctgaaagagg ggcaaaagca ggtatgagga ctttatcaat aggtgcactt
    10141 ttagggctaa atgattttag aaaagaaact ttttttacaa tattgcatgg gaaatactta
    10201 aaaacgaaat atcctcatat tgagctttca tattcaacac caagaatgag accttttaaa
    10261 gggtgttttg aggaattggt agatataagt gatactgatt tagttcaagc tatggtatgt
    10321 atgagattgt ttgaccctca tgcagctata aatatatcca ctagagaaaa tttagaaatg
    10381 agaagtcata taataccttt aggagtaact aaattgagtg caggtgtatc tactgatgta
    10441 ggaggtcatt ctcaagatga acatgatact gctcaattta agataaatga tgaaagtact
    10501 gttaaagatg tggagaaaat gctaaactca attggttatc agcatgtgtt taaagattgg
    10561 gaaagatttt aaatgtatct gataacaaac agaaaattat gtagtgagga acgatattta
    10621 gaagttatta aagagtctat attgagtggt gtagaaaata taataataag agaaaaggat
    10681 ttagaatatc aagagctaaa aaaattatat atgaaaataa aaactaaaat caattgtata
    10741 gattttcaag agcaaatatc agatgaaagt ttgaaaacta atataaatca aaaagagtgt
    10801 agaaataaat ttaaagttaa ttttataata aatagtaaca ttgaattttt tgaaaaagtg
    10861 gattgtcaag gtatccatct tccttttaaa ttattcttga acttaattga aaataagtat
    10921 aattttaatg aaaataaaat actaggatta tctttacata aagtagaaga agtagattac
    10981 ttagaaaaat taataagaaa tcaaaatata aaaatagatt acataacatt atcacatata
    11041 tatgaaacta aatgtaaaga aggattaaat ccaaaaggaa ttgagctttt aaaagaagct
    11101 aaaaagatta ctgatattaa aataattgca cttgggggaa ttttaccatc taatgtcaaa
    11161 gaaacattaa aatactgtga tgattttgct ataatgtcaa ctataatgaa gtctaaagat
    11221 attaaaaaaa ccatatcaaa ttataatgaa aagttgaatt agtaaatata gtatattgaa
    11281 ttttcaactt aaataaaaat aaaaaagtta tcttttaaat tttaaaaggt agctttttta
    11341 ttttatatct ttgctaataa aaaattgtaa aaagggaaaa ctttagtcag agagattaaa
    11401 caatatccta gaaagattta aaatttagta aagtatggag gacattataa tgactgacag
    11461 taaaaagaaa cgatttatta taccacacac ttttacaata atatttttac taatagtctt
    11521 aatggcaata ttgacttgga tagtaccaag tggtgaattt agtagagaag atgtagaagg
    11581 tagaatggta gttgtaccag ggacttatca aagtgtagaa agtaatcctc aaggatttgc
    11641 agatgttttt aaagcaccaa tagaaggctt tatagattca gctgaagttg taggttttgt
    11701 tttaatagtt ggaggtgctt ttggtattgt aaataggact ggagctatag aagcaggaat
    11761 agcttcggtt atctctaagt ttaagggaaa agaacttctt ataattccaa tatcaatgat
    11821 tttatttgga cttggaggta caacatttgg gatggcagaa gagacattac cattttatat
    11881 gatatttgtt ccactcatga catccttggg atatgattct ctaacagcaa tagcaattgt
    11941 atttatagga gcagcagcag gagcagcagc ttcaacaata aatccatttt cagttggtat
    12001 tgcacaggca ctagctcaac ttgctcctgg ttcaggagta acttacagag tagttatttt
    12061 tatagcatat atactaataa gtatagcatt tgttatgtac tatgctaaga aggtaaagga
    12121 aaatcctgaa agttcaatag tatatgaaat agataaaaat aataaatcaa tacttaatca
    12181 aacttcaaca gaaataaaag aatttacttt taaagaagta tctgttcttg ctatatttgg
    12241 aatagggatg gcaataatga tttggggtgt tttaagtcaa ggatggtaca ttccagaaat
    12301 atcaatgtgt tttgctgcaa taggtgtgct ttctggattg gttggtagat tatctcaaat
    12361 tgaaatctca gaaagtttta tagatggagc aaaagattta gcatcagcag ctcttgccat
    12421 agctttggct cgtggaattg taataattgc tcaaaatgga tttataatag atactatatt
    12481 aaattccgca gcagcctttc taggtggcct tccaaaggtt atatttgtat atttatcatt
    12541 tttcttggaa gctgcattgg catttttaat tccatcatct tcaggtcttg cttcacttac
    12601 agtacctgta cttgctcctc ttggtgattt ggttaatata tcagcacaag tgatagtaac
    12661 tgcttatcaa tttggtgtag gtcttacaaa ctttataact ccaacttcag gagtattgat
    12721 gggagcactt gcagtagccc atattccatt ttcaaaatgg gtaagattta taatgcctat
    12781 cttgttgatt ttgacagctg tatctctagt ctttttaaca ataggtattt atatagggtt
    12841 ttaattaggt gtagttttat ttaagatatt tagtaatggt ataaaatgat aatatgtttt
    12901 caataaattg ccactaattt aattagtggt aatttgtttt ttgtatttaa aaataaatat
    12961 atttacttgt gttttgaatt agtatctact tagacgttgt tttaattgtt atattttcat
    13021 ttgattaagc att
//
LOCUS       DAEPXF010000102        13024 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain HB27
            SAMN13376389-rid12479423.denovo.108, whole genome shotgun sequence.
ACCESSION   DAEPXF010000102 DAEPXF010000000
VERSION     DAEPXF010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13376389
            Sequence Read Archive: SRR10518432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 13024)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13024)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001069624.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001069624.1
            Genome Coverage       :: 315x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/15/2021 01:54:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,165
            CDSs (total)                      :: 4,076
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 89
            rRNAs                             :: 10, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 10 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 19 of 69
            Pseudo Genes (incomplete)         :: 44 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 10 of 69
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13024
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13376389-rid12479423.denovo.108"
                     /strain="HB27"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="China: Hebei"
                     /collection_date="2017"
     gene            complement(58..1272)
                     /locus_tag="KRL67_002518"
     CDS             complement(58..1272)
                     /locus_tag="KRL67_002518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HBH1523387.1"
                     /translation="MNQEYVKGNPIFTNINKVPRQFPYLTDDIDTDVIIVGGGVTGCI
                     CAYYLAKNNIKSVILEKGRIAHGSTSVTTSLLQYELDDNLIDLTEVMTLKDALKAYNL
                     CISALEELDTFIELYGNKCDYAKRDTLLYTANKLEIKAIKEEYNLRKENGFDVEYIDE
                     STNPFSFDLKSGLIAKNGGRELDPYKYSHHLIDVSLKNGLQVYENTEVKKVDLSNDKV
                     TAEVSYGYKVHGKKLIVATGYNTSLFTKRNFATKSNTFNIATKPLKNIASWKNNILIR
                     DNCDPYNYLRTTKDNRLIIGGEDVSFDDIENETLANEKYDILEQRLKSMFKDIKDIEV
                     EYKYCGCFASTLDNLGFIGPDNKNNNLWYCLGYGANGILFAILGGIMLSELYLGKQNK
                     NMKLFKVDRFDK"
     gene            1516..2898
                     /locus_tag="KRL67_002519"
     CDS             1516..2898
                     /locus_tag="KRL67_002519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011967718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HBH1523388.1"
                     /translation="MLSKNIASKVLEKCLITGGDFAEIFEEDTINNSISLIDNKVENA
                     IGGRNYGIGIRIFKGLKSIYAYTSDNSLNSLLDVAYKAAVALGKLEDGKSVILNDSIK
                     INNIHNIKIYPNSIGNKDKVSVMKVAYKSAKEFSNDISQVSVSYLDKDQKILIANTEG
                     IYVEDRRIRTRLGISSIASKGNENQTGFEGPGGCKGFEIFEEIDPEYYAKESARVAHT
                     MLHAKNCPAGNMMVAIDNGFGGVIFHEACGHSLEATSVAKGNSVFADKLGQQIASTKI
                     TAIDDGTIPNYWGSMNIDDEGNPAQKNILIENGILKSYMIDKLNGRRMGMNPTGSSRR
                     QNYKFAPTSRMTNTYIDAGEDKPEDIIKSIPDGLYAKKMGGGSVNPVTGEFNFAVSEG
                     YLIKNGEIQEPVRGASLIGKGSDVLMNIDMVGDNLKQAQGMCGSSSGSIPTNVGQPMI
                     RVREITVGGR"
     gene            2910..4259
                     /locus_tag="KRL67_002520"
     CDS             2910..4259
                     /locus_tag="KRL67_002520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HBH1523389.1"
                     /translation="MELKNFKDILFKKALSEGFEECEIYYYTGENLSINIYEGEVEKY
                     NLDKSFGLSFRGKVNGKIGYSYTEILDDKAVDMLIKNVKDGVNTIENEDVQFIYEGDK
                     HYNDVNTYSKELENLEADKLIDLALEMERETKAYSDKVVNLSKCTISYSVSSNGISNT
                     KGLNLSNKTNMLIGFVVPIIEDNGQKYDGIGYNIANSIEEIKPCEIAKLGVDNALSKV
                     GGKSIPSGKYKTILLNEAMVSLLSTFSGIFNADSAQKGLSLLKNREGDMIASPIVTIV
                     DDPLLENGMASTPFDDEGVATFKKEVVSNGTLITLLHNLKTANKAGVKTTGNGIKSSY
                     SSPISIYPTNFYIEKGDKSLDEIIKDIDEGLMVTSFAGLHSGANSVTGDFSLAAKGFY
                     IKEGKKVFPVEQITVAGNYFNLLKDIEAIGEDLEFPMSSIGSPSVVIKELSVAGKDE"
     gene            4410..4943
                     /locus_tag="KRL67_002521"
     CDS             4410..4943
                     /locus_tag="KRL67_002521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese catalase family protein"
                     /protein_id="HBH1523390.1"
                     /translation="MSDAHYDMHKRNGYSSDEPYPEIKVLGPNKYYAELLMDDYAGIS
                     SEFTSVNQYLYHNFDLDETHRELSEMWINISITEMLHMEILAKTIRLLGGNPVYRGST
                     SSCGAYWNGGFVCYGNSICNRLKLDLHLEHVAINNYYKDISLIEDPYIKAILNRIILD
                     EKLHVSLFEKAIEKYCK"
     gene            5027..5836
                     /locus_tag="KRL67_002522"
     CDS             5027..5836
                     /locus_tag="KRL67_002522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBH1523391.1"
                     /translation="MYYVYVNDINIAVYDLNPSAKKTVLFIHGWPLGHKIFEYQTNIL
                     PKLGYRTVSIDLRGFGKSDATSGGYTYSQLADDIYKVVHAIGLKDFTLVGFSMGGAIV
                     LKYMSLFNGYGVSKLVLAAAAAPSFVQRPPEFPYGMTREDVNKLIAQACTDRPEMVTD
                     FGEKVFASNPPESFRRWFNDIGFSASGIGTIGTAVSLRDEELFNDLKCVRVPTGIFHG
                     KLDEICPYEFAVFMNEKIEDSVLYTFEYSGHAIFYDELKLFNQEFLQFLEE"
     gene            6248..8293
                     /locus_tag="KRL67_002523"
     CDS             6248..8293
                     /locus_tag="KRL67_002523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBH1523392.1"
                     /translation="MKKHNILFVSTDDKINIDISKQLENIFGEFCNIDNLVYVNRINI
                     ELSSYELVVCSDNDIKEYIHNNIDKNIPIVIIHRTINIENINQIISIENDSDVMVIDA
                     YKESADETAKIIRKLGLIHINLIPYYPGCDKSKCEIGIITGSRNSIPQNIKQIIDIGD
                     KVIDINTVIEIFTKLNISIDKLHIIKEKYNEDTVSGYRYYTTMNKTMKSFLEIIDEGI
                     ASVDKLGKFIYCNKVFSNLVGIDQNEIISNNFMDLFSDKVVKKIFFQEDEVNDEVVNL
                     NNKKLIINKVNVYENNERIKSIISIKNISAIQVLEDKIQNKFQSKGFVSKYTFESVVG
                     ESKIIKEKINIAKKIATTDFSVLILGENGTGKEIFAQAIHNESLRKNKPFVAVNLSSL
                     SDTLIESELFGYDEGSFTGAIKGGKMGIFERAHTGTIFLDEIGDISLDVQQRLLRVLQ
                     EKEVMRMGGSKIIPIDVRIIAATNKDLKKKIKEGSFREDLYYRINVLHIEIPRLRERK
                     EDIPLISKYFLDEINSNKCFTEESMQALTLYEWPGNVRELKNLIYYIDTIVEEDRVDY
                     EHLPEQFRFEKNNTLVNENFDSIILDFKQSNFFEESICILTSIETWNNKNILLGRNKL
                     QEILKEKDIVLSVDQIRKRIDKLKSHGLLLSGVKKQGSFITDEGKNFISYVKFKGVI"
     gene            8554..9753
                     /locus_tag="KRL67_002524"
     CDS             8554..9753
                     /locus_tag="KRL67_002524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane dipeptidase"
                     /protein_id="HBH1523393.1"
                     /translation="MKSAYKGYEAYSYLEKGKDISSIEMCKGDKRVKEYLIELDDVQE
                     AKVKSIVDEKIFISLHEHPVLFPEHLERDIFEYNREGKQRCAYEALSRGYYDAIFDNL
                     MDGVCTITSNSGWKWDDILIDLGMRLCDLAHQDFVIKCEKVEDIYRAKSEGKVALIPT
                     LEGAAPIENELDRIDILYGFGIRLMGITYSESNALGSGLKEEKDGGLTNFGKKAVERM
                     NKIGMAIDCSHVGVQTTLDVIEYSKDPIILSHVGARSLWNTKRLTPDEVLIACAKKGG
                     VIGVEAAPHTTITEKNKEHSIESFMEHFEYIKNLVGIDHVAFGPDTVYGDHVKLHSVL
                     AAKFDIKKITGTKEHPKVEYVKGLENPTETSKNIVRYLVKHGYSDEEIEKVLGGNILR
                     VLKQVWK"
     gene            9805..10500
                     /locus_tag="KRL67_002525"
     CDS             9805..10500
                     /locus_tag="KRL67_002525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5058 family protein"
                     /protein_id="HBH1523394.1"
                     /translation="MDYLKLANHPLLWIASTVAVSIVVVQSLIFAIKSCKVGKTMGIT
                     DKQIKSAVKSSAISAIGPSMTIFAGMVSLIVTMGGPIAWMRLSFIGSVIFESMSAGFG
                     TDALGITLGSPEMTKLAFTNAVWTMILGSLGWIIFTLLFGHKLDKITNVISSGKKSFI
                     PIISLGAMLGSFAYLNADRVLRFDNGTIACISGMAIMVILCMLEKKKNIKWLREWGLT
                     ISMFSGMIIASVI"
     gene            10513..11190
                     /locus_tag="KRL67_002526"
     CDS             10513..11190
                     /locus_tag="KRL67_002526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH1523395.1"
                     /translation="MSKVKSIYNEEYLPFMIRYGRLTLSLGIIAALVPGIILSFGFGI
                     MPPISALLASTMAIVSMSAPNYIIEPVSYSPILGIPGTYMSFLSGNISNMRLPCSIAA
                     QKAAEVESGTEEGSIISTIGIAVSILVNISILTIGVILGGSVLSKIPAEVVEKLNLIL
                     PALFGSVFGQVFLQDKKLGLVAIVISVLTIILSKQGIIPQSLVVLICVFGTILIARAM
                     YKDKLSD"
     gene            11301..12902
                     /locus_tag="KRL67_002527"
     CDS             11301..12902
                     /locus_tag="KRL67_002527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HBH1523396.1"
                     /translation="MIYDLILKNGFIIDGTGNPGFYGDIAIKDNLIAKIDSKINGNAN
                     KDIDCCGKVITPGFIDPHVHEEIVAILDGKFEKFLKQGVTTTINGNCGHSITPYSSEN
                     IYEYMYKNGLLLEEEKKYLIDKNKCWNNFTEYCDLISKSGISVNMGFLLGHGTIRWSV
                     MGGSKDRPPTEKEKNEITDIINDGMKSGAFGISTGLAYIPSKYADIDELVDIARQIKE
                     HDGIYTSHIRDYIGRYNAVKEAIEVGQKSGARVQVSHLSPVEIEAFDEILKARYNGVE
                     IMVDTVPRSSGHCMKKKRVIQFIMAISSSLFELGIDGVMDALRNEEGRALILKEAFIL
                     GDRGSIILLNTKDINMEKKSIREIATQKGIDEDKLLLDLLLDGDEELIFCLGGMYRAD
                     FPDKLHDNKIIDNPFVMVGSDCLFSVGGDMSWFELQRRGAFPIFFNMYRKSGVRLEEI
                     VRRVTSLPARQFKIKNRGILKDGLIADIAVIDMDNYSYPRSEDIDFSKPQLLANGVEY
                     VIINGKIALENGEITENKCGEVLKR"
BASE COUNT         5016 a         1379 c         2355 g         4274 t
ORIGIN      
        1 agttaacttt tagaattata aagtttcatc tttttacagt cttttaatac ataatatcta
       61 tttatcaaat ctatctactt taaataattt catattttta ttttgtttgc caagatataa
      121 ttcacttaac attatacctc ctaatattgc aaagagtata ccatttgctc cataacctaa
      181 gcaataccat agattgttat ttttattatc tggaccaata aaacctaaat tatctaaagt
      241 tgaagcaaaa caaccacaat atttatattc aacttcaatg tcttttatat ccttaaacat
      301 acttttcaat ctttgttcta aaatatcata cttttcattg gctaaagttt cattttctat
      361 atcatcaaaa cttacatctt caccacctat aattaatcga ttgtctttag tagttcttag
      421 ataattatat gggtcacagt tatctctaat taaaatgttg tttttccaag aagctatgtt
      481 tttaagtggt ttcgttgcta tattaaaagt atttgactta gttgcaaaat ttctttttgt
      541 aaataaactt gtattgtacc ctgtagctac aattaatttt ttaccatgaa ctttgtatcc
      601 atacgatact tcagcagtaa ccttatcatt tgataaatct acttttttta cttctgtatt
      661 ttcatatact tgaagaccat tttttaaact tacatcaatc aaatggtgac tatatttata
      721 tgggtctaat tctcgtcctc cattctttgc tatcaatcct gattttaaat caaagctaaa
      781 ggggtttgta gattcatcaa tatattcaac atcaaatcca ttttcttttc ttaagttata
      841 ttcttctttg atagctttaa tttcaagttt attggcagta taaagtagag tatctctttt
      901 ggcataatca cacttatttc catatagctc tataaatgta tctaattctt caagtgctga
      961 aatacaaagg ttatatgctt ttagagcgtc ttttagagtc ataacctctg ttaaatctat
     1021 caaattgtca tctaattcat actgtaatag agaagttgtc acgctagtgc ttccatgtgc
     1081 aatccttcct ttttcaagaa tgacagactt tatattgttt ttagctagat agtaagcaca
     1141 aatacatcca gttacgccac caccaacaat tataacatca gtatcaatat catcagtaag
     1201 gtatggaaat tgtcttggaa ctttattaat attagtaaaa ataggatttc cttttacata
     1261 ttcttgattc atatacttca ctcctaaaat ttattatgta tgagtgtatt attttctata
     1321 gcaaataata ttatacattt taattatcta gttatatggg tatgtataga taaaatgtga
     1381 taacatttta gtataaaaca tatgtataat atattcataa ttaccttatt ttaagtaata
     1441 tagtatataa tgtttgttaa caagtaaaaa gattgaataa aagttaatta aaaatttaaa
     1501 gtaggaggta aatttatgct ttcaaaaaat atagcttcta aggtattaga aaagtgtcta
     1561 attactggag gagattttgc tgagattttt gaagaagaca ctataaataa ttctattagt
     1621 ttaattgata ataaagtaga aaatgcaata ggtggaagaa attatggtat aggaataaga
     1681 atatttaaag gattaaagag tatatatgct tatacaagtg acaattcatt aaatagtctt
     1741 ctggatgtag catacaaagc tgctgttgct ttaggtaaat tggaagatgg aaaaagtgta
     1801 atattaaatg acagtatcaa aataaataac atacataata taaaaattta tccaaattca
     1861 ataggaaata aagataaagt gagtgttatg aaggttgcat ataagagtgc aaaagaattt
     1921 agtaatgata tatctcaagt aagtgttagc tatttggata aagaccaaaa aatattaatt
     1981 gcaaacactg aaggtatata tgttgaagat agaagaataa gaacaagact ggggataagt
     2041 tcaattgcat ctaagggtaa tgaaaatcaa actggatttg aaggtcctgg aggttgtaaa
     2101 gggtttgaaa tatttgaaga aatagaccca gaatattatg caaaagaatc agctagagta
     2161 gcgcatacta tgttgcatgc taaaaattgt cctgcaggta atatgatggt agcaatagac
     2221 aatggatttg gtggagtaat atttcatgaa gcttgtggac attctttgga agctacatca
     2281 gttgcaaaag gtaattctgt atttgcagat aaattaggtc aacagattgc ttctacaaag
     2341 attacagcta tagatgatgg tacaatacca aactactggg gttctatgaa tatagatgat
     2401 gagggaaatc cagctcaaaa aaatatactg attgaaaatg gaattttgaa gtcttatatg
     2461 atagataaac ttaatggtag acgaatggga atgaatccta caggaagttc tcgtagacaa
     2521 aactacaaat ttgctccaac ttcaaggatg actaacactt atattgatgc tggagaagat
     2581 aaaccagaag atattataaa gtcaatacca gatgggcttt atgctaaaaa aatgggtggt
     2641 ggttctgtaa atcctgtaac aggggagttt aattttgcag tttcagaagg ttacttaata
     2701 aaaaatggag aaatacaaga gccagttaga ggtgctagtt taattggaaa aggcagtgat
     2761 gtacttatga atatagatat ggttggggat aacttaaaac aagctcaggg catgtgtggc
     2821 tcatcatcag gaagtatacc aactaatgta ggtcaaccta tgataagggt aagagaaatt
     2881 actgtaggtg gaagatagga gggataattg tggaacttaa aaattttaaa gatatattat
     2941 ttaaaaaggc tctaagtgaa ggttttgaag agtgtgaaat atattattat acaggagaaa
     3001 atttaagtat aaatatatat gaaggagaag ttgagaaata caatcttgat aaatcttttg
     3061 gtttatcttt tagaggtaag gtaaatggaa agataggtta ttcttataca gaaattttag
     3121 atgataaagc tgttgatatg ttgataaaaa atgtaaaaga tggagtaaac actattgaaa
     3181 atgaagatgt acaatttata tatgaaggag ataagcacta taatgatgta aatacttatt
     3241 ctaaagagct agaaaattta gaggcagata agttgattga tttagcttta gaaatggaaa
     3301 gagagactaa agcttattca gataaggtgg taaatttatc taaatgtact atatcttatt
     3361 ctgtatcaag taatggtata tctaatacta aaggtcttaa tttaagcaat aaaactaata
     3421 tgcttatagg gtttgtagtt cctattatag aagacaatgg acaaaaatat gatggaatag
     3481 gatataatat agcaaattct attgaagaaa taaaaccttg tgagatagca aaacttggtg
     3541 ttgataatgc tttatctaaa gtaggtggta agagtattcc atcaggtaaa tataaaacta
     3601 tattgcttaa tgaagctatg gtatctttgc ttagcacatt ttctgggata tttaatgcag
     3661 attcagctca aaaaggatta tctttattga aaaatagaga aggagacatg atagcatctc
     3721 ctatagttac aatagttgat gacccgcttt tagaaaatgg tatggcatct accccttttg
     3781 atgatgaagg agtagctaca tttaaaaaag aagttgtttc aaatggaacg cttataactt
     3841 tacttcataa cttaaaaacg gctaataagg caggtgtaaa gactacagga aatggaataa
     3901 aatcttcata ttcatcacct ataagcatat atccaactaa tttttatata gagaaaggag
     3961 ataaatcttt agatgaaatt ataaaggata tagatgaagg attgatggtt actagctttg
     4021 caggacttca ttcaggtgca aattcagtaa caggagactt ttctttagct gccaaaggtt
     4081 tttatatcaa agaaggaaaa aaagtatttc cagtggagca aattacagta gcaggaaact
     4141 attttaacct gttgaaagat attgaagcta taggggaaga tttagaattt ccaatgagta
     4201 gtataggttc accttcagtt gttattaaag agctatcagt agctggaaaa gatgaataat
     4261 taaaaaagta attttgtgga taaaaggtca tgttaatttt aattaatatg gctttttatt
     4321 atttaagtag attttattat ctaagttata tgaaaaagtc gtgatgcata taattaatat
     4381 catgaattaa atttaaagga ggagatatta tgagtgatgc acattacgac atgcacaagc
     4441 gaaatggata ttcaagtgat gagccatatc cagagataaa agttttaggt ccaaataagt
     4501 attatgcaga attattgatg gatgactatg ccggaatatc aagtgaattt acgagtgtaa
     4561 atcaatatct ataccataat tttgatttag atgaaacgca tagagagtta agtgaaatgt
     4621 ggataaatat atctataaca gaaatgttac atatggagat attagcaaag actataagat
     4681 tattgggtgg aaatcctgta tatagaggct ctacaagttc ttgtggtgca tattggaatg
     4741 gtggttttgt atgttatgga aattctattt gtaacagatt aaaactagat ttgcatttgg
     4801 agcatgtggc tattaataat tattataaag atataagtct tattgaagac ccttatataa
     4861 aagctatatt aaatagaata atcctagatg aaaaacttca tgtaagttta tttgaaaagg
     4921 caatagagaa gtattgtaaa taaatttaaa aatttagtta ttcatcattt tattcatata
     4981 tataaaataa ccatcataca ttttcttgaa gggagttgct gttgatatgt attatgtata
     5041 tgtgaatgat ataaatatag cagtatatga tttgaatccg agtgctaaaa aaactgtact
     5101 ttttatacat ggctggccat taggacataa gatatttgaa tatcaaacaa atatattgcc
     5161 aaaactcgga tatagaacag tttcaattga tttaagaggt tttggtaaat ctgatgcaac
     5221 atcaggagga tatacttata gccaattagc agacgatatt tataaggtgg tacatgctat
     5281 tggattaaaa gactttaccc ttgttggctt ttcaatggga ggagcaatcg tacttaagta
     5341 tatgagttta tttaatggat atggagtgtc aaaattggta ttagctgcag ctgctgcacc
     5401 atcatttgta caaagacctc cagaatttcc atatggtatg acacgtgaag atgtaaataa
     5461 attgatagca caagcatgta ctgatagacc agaaatggta acggattttg gcgaaaaagt
     5521 atttgcatca aatccaccag aaagctttag aagatggttt aatgatattg gttttagtgc
     5581 atcaggtatt ggcactattg gaactgctgt atctttaaga gatgaagaat tatttaatga
     5641 tttaaaatgt gtaagagtgc ctactggtat atttcatggg aaattagatg aaatatgtcc
     5701 gtatgaattt gcagttttta tgaatgaaaa aatagaagat tcagttttat atacttttga
     5761 gtatagtggt catgctatat tctatgatga gcttaaacta tttaatcagg aatttttaca
     5821 gtttttagag gaatagatat attttattat cacttagaaa aagagtaata ttatatgaaa
     5881 tgatttaagc taaattttat gatatttttt acactaaaca aatagggttt tagaacttgt
     5941 aattaagttc tgagacccta ttattatata ttcctaaagt ttgttacaat ttattcctta
     6001 taattcttga aaatagtgtt aatatattaa cactattata ttaaattttt atttctattc
     6061 caataatgtc aaaataattg ttttagaaac taaattgtat gtgaaaaaag atagcttaca
     6121 ttattttatt gtggaaaata atagtaaatg gtacaaaatt tgatttttaa gatttaaaac
     6181 ttttttaaaa agtgctacaa tagaccaaat tattactttc tatactcgtt aaacaggagg
     6241 tgggactatg aaaaaacata atattttatt tgtatcaact gatgacaaaa taaatataga
     6301 tatatcaaaa caattggaaa atatatttgg agaattttgt aatatagata acttggtata
     6361 tgtaaatagg ataaatattg aactgtcaag ctatgagctt gtagtatgtt cagacaatga
     6421 tataaaggaa tatatacata ataatattga taaaaatata cctattgtaa taatacatcg
     6481 tactataaat attgaaaata taaatcagat aataagtata gaaaatgatt ctgatgttat
     6541 ggttatagat gcttataaag agtcagcaga tgaaactgcc aagataatta gaaaactagg
     6601 attaattcat attaatctaa taccatatta tccaggttgt gataagtcta aatgtgagat
     6661 tggaattatt acgggaagca gaaacagtat accacaaaac ataaagcaaa taattgacat
     6721 aggtgataag gtaattgata taaatacagt tatagaaatc tttacaaaac taaatatatc
     6781 tatagataaa ttacatatta ttaaggaaaa atacaatgaa gatacagtaa gtggatatag
     6841 atattataca actatgaata aaaccatgaa gagttttttg gagattatag atgaaggtat
     6901 tgcttctgtt gataaattag gaaaatttat atattgcaac aaagtattct ctaatctagt
     6961 tggaatagac caaaatgaaa ttatatctaa taactttatg gacttattta gtgataaggt
     7021 tgtcaaaaaa atattttttc aagaagatga agttaatgat gaagtagtaa atttaaataa
     7081 caaaaaactg ataattaata aagtaaatgt ttatgaaaat aatgaacgaa taaagagtat
     7141 tataagcatt aaaaatataa gtgcaattca agtgcttgag gacaagattc aaaataaatt
     7201 tcagtcaaaa ggttttgtat caaagtatac atttgagagt gttgtaggtg aaagtaaaat
     7261 aataaaagaa aaaattaata ttgcaaaaaa aatagcaact acagactttt ctgtgttgat
     7321 acttggagaa aatggaacag gaaaagaaat atttgcccag gcaatacaca atgaatctct
     7381 aagaaaaaac aagccattcg ttgcagttaa tctatcttct ttgtcagata cacttattga
     7441 aagtgaactt tttggctatg atgaaggaag ttttacagga gcaataaaag gtggaaaaat
     7501 gggtatattt gaaagagcac atacaggaac aattttttta gatgagatag gtgatatatc
     7561 tttagatgta cagcagagat tgctaagagt attacaggaa aaagaagtta tgagaatggg
     7621 cggaagtaaa attataccaa tagatgttag aattatagct gcaacaaata aagatttaaa
     7681 gaaaaaaatt aaagaaggtt cttttagaga agatttgtat tataggatta atgtattaca
     7741 tatagagata cctagactta gagaacgtaa ggaagatata cctcttataa gtaagtattt
     7801 tttagatgaa ataaatagca acaaatgttt tacagaggaa tcaatgcaag ctttaacatt
     7861 gtatgaatgg ccaggaaatg taagagagct taaaaattta atttattata ttgatactat
     7921 agttgaggaa gatagagtag attacgagca tttgccagaa caatttagat ttgagaaaaa
     7981 caatacttta gttaatgaaa atttcgattc aattatcctg gatttcaaac aatcgaattt
     8041 ctttgaggaa agtatatgta tattgaccag tatagaaact tggaataata aaaatatatt
     8101 attaggaaga aataaactgc aagaaatttt aaaagaaaaa gatatagttt taagtgtaga
     8161 ccaaattaga aaaagaatag ataagttaaa gagccatggt ttacttttat ctggagtaaa
     8221 aaaacaaggt agttttataa ctgatgaagg aaagaacttt atatcatatg taaaatttaa
     8281 gggagtgata taggggacta aaaaggcaaa acaccctaaa aaaatcccta taatagatag
     8341 aaataaataa aattatcttt aagattaaga taattcaaaa atattgaata aataatctaa
     8401 aaagattatt tttaataaaa cagtagtaaa tatcaacctt actactgttt ttattttttg
     8461 caagatttat tatacaatta aaatttatat tttggcatat caattgctat ataggaaaaa
     8521 tataaaaaat tgtagaactt ggaggatgtt gttatgaagt cagcttataa aggatatgaa
     8581 gcctatagtt atttagaaaa aggaaaagat atttcatcta ttgagatgtg taaaggagat
     8641 aaaagagtta aagagtacct tattgaatta gatgatgttc aagaagctaa agttaaaagt
     8701 atagtggatg aaaaaatatt tatatcatta catgaacatc cagtattatt tccagaacac
     8761 ttagaaagag atatatttga atacaatagg gaaggtaaac aaagatgtgc ttatgaagca
     8821 ttatcaagag gatattatga tgctatattt gataatttaa tggatggtgt atgtacgata
     8881 acatcaaatt cagggtggaa atgggatgat atattaatag atttaggcat gagactatgt
     8941 gatttagctc atcaagattt tgtaataaag tgtgaaaaag tagaagatat atacagagct
     9001 aaaagtgaag gtaaagtagc attgataccg acattagaag gtgcagcacc aatagaaaat
     9061 gaacttgata gaatagatat actgtatgga tttggtataa ggttaatggg tataacgtac
     9121 agtgaatcca atgcattagg ttcaggatta aaagaagaaa aagatggagg tcttacaaat
     9181 tttgggaaaa aagctgttga aagaatgaat aaaattggaa tggcaataga ttgttctcac
     9241 gttggagtcc aaactacact tgatgtaata gaatatagca aggacccaat aatattaagc
     9301 catgttgggg caagaagttt atggaacaca aagagattaa ctccagatga agtattaata
     9361 gcatgtgcta aaaaaggagg ggttataggt gtagaagcag cacctcatac tacaataaca
     9421 gagaagaata aagaacattc aatagagtca tttatggaac attttgagta tattaaaaat
     9481 ttagtaggaa tagaccatgt agcttttgga ccagatacag tatatggcga ccatgtaaaa
     9541 ttacactcag tactagctgc taaatttgat ataaaaaaaa taacaggtac aaaagaacat
     9601 cccaaagtag aatatgtaaa aggtcttgag aatccaacag aaacttcaaa aaacattgtc
     9661 agatatttag taaagcatgg atatagtgat gaggaaatag agaaggtatt aggtggaaat
     9721 atacttagag tattaaaaca ggtttggaaa taaatatatt agaaatatta ataaaatttg
     9781 aggatttata gggaggtttt ataaatggat tatttaaaat tagctaatca tccacttttg
     9841 tggatagcga gtacagtagc agtatcaata gtagtggtac aatcccttat atttgctata
     9901 aaatcttgca aagttggtaa aacaatggga ataacagata aacaaataaa gtcagctgtt
     9961 aagagtagtg ctatttcagc tattgggcct tcaatgacta tatttgctgg aatggtatcg
    10021 cttatagtaa ctatgggtgg gcctatagca tggatgaggt tatcatttat aggttctgta
    10081 atttttgaat caatgtcagc cggatttgga acagatgcat taggtataac tttaggttct
    10141 cctgaaatga caaaacttgc atttacaaat gcagtttgga ctatgatatt aggttcttta
    10201 ggatggataa tattcacatt actatttgga cataaactag ataagataac caatgttata
    10261 tctagtggca agaaatcatt tataccaata atatctctag gagcaatgtt gggttcattt
    10321 gcatatctaa atgccgatag ggtgttgcgc tttgataatg gaacaattgc atgtataagt
    10381 ggaatggcta taatggtaat attgtgtatg cttgaaaaga agaaaaatat aaaatggcta
    10441 agagagtggg ggcttacaat atcaatgttt agcggaatga taatagcttc agtaatataa
    10501 ggggggagtt ttatgagtaa agtaaaatct atatataatg aagaatattt accttttatg
    10561 ataaggtatg gaagattaac actatctttg ggtattatag cagcattagt ccctggaata
    10621 attttgagtt ttggatttgg tattatgcct ccaatatcag cattattagc aagtacaatg
    10681 gcaattgtca gtatgagtgc tccaaattac ataatagaac cagtttctta ttcaccaatc
    10741 ttaggtatcc caggaactta tatgagtttt ctttctggta atatatctaa tatgaggtta
    10801 ccatgttcaa tagcagctca aaaggcagca gaagttgaat caggtacaga agaaggctcg
    10861 ataatatcta caataggaat agctgtatct attttagtga atataagtat actaactata
    10921 ggcgtaattt taggaggaag tgtattatct aaaattccag ctgaagtagt agaaaaatta
    10981 aacttaattt taccagctct atttgggtca gtttttggac aagtattttt acaagataaa
    11041 aaattaggtc tagtagcaat tgtaatatct gtattaacta ttatattatc aaaacaaggt
    11101 ataataccac agtctctagt agttcttata tgtgtatttg gtactatatt aattgcaaga
    11161 gctatgtata aagataagct tagtgattaa ttacaatgaa gtaatagtga attttcaaat
    11221 atgaaatata ataatttgat tttcaaatat aaaaattaca ttaagattat ttaaaaattg
    11281 gtttttaagg aggggatatt ttgatatacg atttaatctt aaaaaatgga tttataatag
    11341 atggtacagg taatcctggt ttttatggag atatagcaat taaagacaac ttaatagcaa
    11401 aaatagattc taagataaat ggcaatgcaa ataaagatat tgattgttgt ggaaaagtta
    11461 taacacctgg atttattgac cctcatgttc atgaagaaat agtagcaata ctagatggta
    11521 aatttgaaaa gtttctaaaa caaggagtta caacaactat aaatggtaat tgtggacatt
    11581 ctataacacc atattcttct gaaaatatat atgaatatat gtataaaaat ggcctattac
    11641 ttgaagaaga aaaaaagtat cttatagata aaaataaatg ttggaataat tttacagaat
    11701 attgtgacct tatatctaaa agtggtattt ctgtcaatat ggggttcttg ttggggcatg
    11761 ggactataag atggagtgtt atgggaggtt ctaaggatag acctcctaca gaaaaggaaa
    11821 aaaatgaaat tacagatata ataaatgatg gaatgaaaag tggtgctttt ggaatatcta
    11881 ctggattggc atatatacca agtaagtatg ctgatattga tgaacttgtt gatattgcaa
    11941 ggcagattaa agagcatgat ggtatatata catctcatat tagagattac ataggaagat
    12001 ataatgcagt aaaagaagcc atagaggttg ggcaaaaaag tggagctagg gttcaagtat
    12061 ctcacttaag tccagttgaa atagaagcat ttgatgagat attaaaggct aggtataatg
    12121 gtgttgaaat aatggttgac acagtaccaa gaagttctgg gcattgtatg aagaaaaaaa
    12181 gagtaattca atttattatg gcaatatcat ctagtctatt tgaattaggt atagatgggg
    12241 taatggatgc tcttagaaat gaagagggaa gagcacttat attaaaagaa gcatttatac
    12301 ttggagatag gggaagtata atacttctaa acactaaaga tattaatatg gaaaagaaat
    12361 caatacgtga aatagctaca caaaaaggaa tagatgagga caagttatta ctcgatttac
    12421 ttttagatgg agacgaagaa cttatctttt gtttaggagg aatgtataga gctgatttcc
    12481 cagataaact acatgataat aaaataattg ataatccatt tgttatggtt ggttcagatt
    12541 gtttgtttag tgtaggtggg gatatgtcat ggtttgaact acaaagaaga ggagcttttc
    12601 caattttctt caatatgtac agaaagtctg gtgttagatt ggaagagatt gtaagaagag
    12661 ttacttcatt accagctagg cagtttaaaa taaaaaatag aggtattctt aaagacgggt
    12721 tgatagctga tatagcagtt attgacatgg ataactatag ttatcctagg tcagaagata
    12781 ttgatttttc aaagccacaa ttactagcca atggtgttga atatgtaatt ataaatggaa
    12841 aaattgcgtt agaaaatgga gagattactg agaataaatg tggtgaggta ttaaaaagat
    12901 aaggttttaa aaataatagg atttagaaag taaataaata tctaagtaag taaagacgaa
    12961 gtttgagatt tatttaaata aaaatcatgc ttcgttttta tttatagtat taatatttat
    13021 ttta
//
LOCUS       DAEPXF010000103        12975 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain HB27
            SAMN13376389-rid12479423.denovo.109, whole genome shotgun sequence.
ACCESSION   DAEPXF010000103 DAEPXF010000000
VERSION     DAEPXF010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13376389
            Sequence Read Archive: SRR10518432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 12975)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12975)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001069624.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001069624.1
            Genome Coverage       :: 315x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/15/2021 01:54:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,165
            CDSs (total)                      :: 4,076
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 89
            rRNAs                             :: 10, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 10 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 19 of 69
            Pseudo Genes (incomplete)         :: 44 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 10 of 69
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12975
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13376389-rid12479423.denovo.109"
                     /strain="HB27"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="China: Hebei"
                     /collection_date="2017"
     gene            complement(279..425)
                     /locus_tag="KRL67_002528"
     CDS             complement(279..425)
                     /locus_tag="KRL67_002528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH1523397.1"
                     /translation="MTDKIIKPKNLFIYELKCDLIEDDERFVIKGKLDKEEINNLIFY
                     LQGV"
     gene            complement(443..3424)
                     /locus_tag="KRL67_002529"
     CDS             complement(443..3424)
                     /locus_tag="KRL67_002529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HBH1523398.1"
                     /translation="MLPKEKHLEESIENHLVSIGYEQGHSQEFDLENCLFVEDLFRFL
                     EITQKELLDEFKYNRGSNYKKAFIDLINSNIKRRGLISVLRNGVEDIMMNGKFQLFYN
                     KPNLTTNKTAYENYKQNIFKITRQLKYSSKHNNTLDIVLSLNGFPVIVMELKNQFTNQ
                     NVYNAIKQFKQDRNPKETLFGFNQRVFVYFAVDTDEVYMTTELKGDKTFFLPFNKGNN
                     MGKGNPVVEGKEKTSYLWEEILTKDSLLDIIKRFYFIQEDDKKSKRAIFPRFHQLDAV
                     RKIVADLEVNKAGKNYLIQHSAGSGKTNTIAWTAHRLSSLHDEDNNAIFDSVIVVTDR
                     KVLDKQLQDAIYQLEHKNGLVVKIDDDKNSSDLADAIVNRAKIIITTIQKFQYALPKI
                     EQLEKRRYAVIIDEAHSSTSGENMNALKETLAGKTLEEAKIIDAENEKNEKSSIDKMN
                     DLIEKRTNLDHISFFAFTATPKNKTMQVFGRIGEDGLPHEFHLYSMKQAIEEGFILDV
                     LKNFMPVSVYYKVGKKIEDNPEFDKGEAKRAINKFISLNEHNIRQKVETIVDDFVDNR
                     SMWLEGRAKGMIVTASRLHAVKYKLAIDQYIKEKGYNMKSLVAFSDTVKDEDIEYTEV
                     GMNKEVALDINDSNLPSIFEKSDFRLLIVAEKYQTGFDQPKLCAMYVDKKLDGVKTVQ
                     TLSRLNRTYPNKHTFILDFQNSVEDIQEAYKPYFEMTNIDKVTEPNVVNDLWYQLHEY
                     GIYTDEEVNDFAVLFYKDKRTNTQDAKMNSLIDKAVERYCYLDEEDEKSADEFKKKCQ
                     KYITFYNFLIQIYPIKNLNLLRLQVYLVALLRKLPKKDKERIDIDDILSLDYYKLQKL
                     GEDGKKGSDISLYNGEGELVGISETATSRVSEADEEYLEDIIQKINDVFGVGLTEEDR
                     IIIDQYEEMFKNDKSIMDIAKANSYEDLVSTFGKSYFKRGIVKTKNMNDRLVKEILTN
                     NNLQNYMIHYLAEKIYAEANR"
     gene            complement(3486..4388)
                     /locus_tag="KRL67_002530"
     CDS             complement(3486..4388)
                     /locus_tag="KRL67_002530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH1523399.1"
                     /translation="MKLRQNYYELEEFIHREIQDYVCKSAQEGFKLSIQSSYKLYKNL
                     HTNTIRADIQKERVNIVNAIPIYNTSQKNNKKYTPDIILYTDNNREIYCEISVYKSVN
                     TADYFEEWKVLGKTVIEIRKTEIMDMHLDGYRLSKDLIISYLYDPILDKARKQKISES
                     QKIIDKSNNIISKHKNLNKENNVKENNSKEKIREKEYKEFDDKVNYDIKKHLNNLNEN
                     NILIKEYVDKRTDKQKRSDKRNGREPKKELVYFDVKSNKEARIIYAVIMKNSKRAYRS
                     IPAIIGKYLRDDGYKVGVAGKKRK"
     gene            complement(4464..4784)
                     /locus_tag="KRL67_002531"
     CDS             complement(4464..4784)
                     /locus_tag="KRL67_002531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH1523400.1"
                     /translation="MLYVGDYIDMKTLNIRYKDGFKIEEDTRLYPINSDINDKIGVLV
                     EHRKLNATNRYLRYGIQKISGKCYFFDVRNSVISKTKESAYNTFICPYCRERMFIVSA
                     FERQ"
     gene            complement(4818..5270)
                     /locus_tag="KRL67_002532"
     CDS             complement(4818..5270)
                     /locus_tag="KRL67_002532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH1523401.1"
                     /translation="MKLSRKNITMQVLSKFIENNLEKIENVETKANQIVFRTIDGDKK
                     IKIKTSKNYSEGEDPNTYLWTSFSSEELEEDYDYYTIICNEDIEKAVVFTKEELKQHY
                     VLETKKKKNGEIDFELYPHFIGRRCIDARVHLYKEPIDITKYVNNYIF"
     gene            complement(5302..6669)
                     /locus_tag="KRL67_002533"
     CDS             complement(5302..6669)
                     /locus_tag="KRL67_002533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HBH1523402.1"
                     /translation="MRYRYRDDEEMKDSGVEWIGKIPKDWEVKRIKHLFELKKDKSDE
                     ENPTVLSLTQKGLKIRDVSNNEGQLASTYVGYTKIEKNDFILNPMDLISGYTDKAEIE
                     GVISPAYTTFRSKNKVNINHDYYKRYFQMHYHHNFLFPWGEGVSFEHRWTLKNEVFLN
                     LPVITNRLEEQEKIANFLDEKTSQFEFIISKKEELIKKLEEAKKSLISEVVTGKVKVV
                     KTDDGYKLVKRSSEEMKDSGIEWLGEIPKDWEVKNFKYMFTLNKGLSITKADLKESGI
                     PCVNYGEIHSKYRFELKPSKHKLKYVDESYLKSNSISLLKYGDFVFCDTSEDIEGCGN
                     FTYLNENNKVFAGYHTIIARTLEQVNYRYMSYLFDSNDWRIQIRTKVSGVKVFSITQS
                     ILKGTKVILPDLLEQRNIAQYLDSKCKGIDSIIDKTKLQIDKLKEAKQSLISEAVTGK
                     IEILD"
     gene            complement(6669..8696)
                     /locus_tag="KRL67_002534"
     CDS             complement(6669..8696)
                     /locus_tag="KRL67_002534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent DNA methyltransferase"
                     /protein_id="HBH1523403.1"
                     /translation="MSIDNNFNHIAFLWNIAESLRGTYKEEDYRKVMLPLIVIRRFDC
                     LLDDYDREIVKSVYKEYDFLPEEEKDELVIVDLKENHNIDLQFYNVSDFTWKKLLDDS
                     ENIKSNFEEYLNGFSNNVKEIIGKFKFKDEIAQLDKKDKLYAVLSKMYEVDLHINSVS
                     NNEMGYIYEEMLRRFTENSAAGEQYTPREVIRLCMEMLFMGKENFLTEEGKVISIADF
                     CCGTGGMLSIAEDYVEKVNPSAIVNVYGQELLDESFAICQADMIMKGQNPDNIRLGNT
                     LTQDRFSGEKIRFLISNPPFGVTWKDEEKKVKEEADLGFDGRFGAGTPRVSDGSLLFL
                     QNMISKMYDDEEGSRIAIIFNGSPLFTGDAGSGESNIRKWIIENDLLEGIIALPTDMF
                     YNTGIATYIWVLTNKKEDKRKGKIQLVNASEYYQLMRKSLGNKRKEISLEQIEEIKEI
                     YERFEESENSKIFDNEGFGYRKVTIERPLKLSFRVNEEAIENVKNTTQFINLSVSKKK
                     DEEVKVKEEAEGRVKQDKLLKLLESFDSEFEYMKRNKFIKDLKSKSKLYDVALSAGLI
                     KAIVNAIGVRNEDAVVCKDAKGNIESDSSLKDTESIALKEDVYEYFEKEVKPHVEDAY
                     IDESSIDNIGYEIPFTRYFYKYEKLKSFDDIMKEVESLESEIALEIRKVLG"
     gene            9158..9637
                     /locus_tag="KRL67_002535"
     CDS             9158..9637
                     /locus_tag="KRL67_002535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH1523404.1"
                     /translation="MFLNDIFDIIKFNKKSPIEQLYVIQEEEKPDIKEAEEYYRCNYR
                     FYNSLKEMDSIENIKTEKTRIKTVLDSKNNPSIPFAIAIIAAFISIFFSLLTNNIIKD
                     FFTACSVIGVFFGTYLCLICESQQKWDSRQKFYMSSLQVLDNMESKKLKERKYKKRR"
     gene            complement(9666..10478)
                     /locus_tag="KRL67_002536"
     CDS             complement(9666..10478)
                     /locus_tag="KRL67_002536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HBH1523405.1"
                     /translation="MKICITVGHSILKSGACTSADGVVNEYQYNKSLAPVLADVFRKE
                     GHKVDIIICPEKQFKTKSEEKTYKIPKVNSGGYDLLIELHLNASDGQGKGSEVLYYSN
                     KGLEYATRICKKLGTVFKNRGAKLDKGLYILNSSKPTAVLIESFFCDNKEDYEKAKKL
                     GHEGIAKLIVEGVLNKNINSEGVKQMYKHTIVYDGEVDKIPATVVGWGYNDGKILICD
                     IKDYVPGQTQNLYVIGGAACEKIGTITKEHYTMIKGNDRFDTLYKALDFIDR"
     gene            complement(10478..10735)
                     /locus_tag="KRL67_002537"
     CDS             complement(10478..10735)
                     /locus_tag="KRL67_002537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HBH1523406.1"
                     /translation="MDNLISFIPEQLLILVAALYVIGAGCKKYKQLDNKYIPVILLTL
                     GVGFSVWMLGLNPSAVLQGVICWGISIGINQTYKQLKEENK"
     gene            complement(10755..10985)
                     /locus_tag="KRL67_002538"
     CDS             complement(10755..10985)
                     /locus_tag="KRL67_002538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin XhlA family protein"
                     /protein_id="HBH1523407.1"
                     /translation="MNEELFEADLKRHETRINKHGEEIDELKIANIESKAELKALCEN
                     LNSLTSMLKWLIGTMITTLVGFFIFAIQKGIF"
     gene            complement(11056..11589)
                     /locus_tag="KRL67_002539"
     CDS             complement(11056..11589)
                     /locus_tag="KRL67_002539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH1523408.1"
                     /translation="MTFKELVNKVRNLVLEAKNVTIEDTENNFTSDNVEGALKECIDR
                     ADLAFQSADSGKELIATAIGSPATKDNTFAELASLIGDRKKFKMGNIQLTMEQKKANL
                     EIDFEPEILIAFIGEIDYSRYNTATYYKEGVLLLEGRINSISFKILNENSIYSIVMNP
                     PYNVSTAPLGVTWYAIK"
     gene            complement(11623..11805)
                     /locus_tag="KRL67_002540"
     CDS             complement(11623..11805)
                     /locus_tag="KRL67_002540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH1523409.1"
                     /translation="MNINNVVVRILAERILGGGLNPLKNREFELDDVTNVEYRKAVED
                     YIIRESGVVEGAEPTI"
     gene            complement(11805..12098)
                     /locus_tag="KRL67_002541"
     CDS             complement(11805..12098)
                     /locus_tag="KRL67_002541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH1523410.1"
                     /translation="MNRANRIIYDQTGKILLQTGEATGDILEHDTITKLHCIDIPYGS
                     IDYMQNRIVGINIETKEPILEEIPIFVTEEEKRIQELENQILIAENEKVGGIL"
     gene            complement(12385..12975)
                     /locus_tag="KRL67_002542"
                     /pseudo
     CDS             complement(12385..12975)
                     /locus_tag="KRL67_002542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112542.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         3849 a         2307 c         1307 g         5512 t
ORIGIN      
        1 tagattcctt atttattttg aagttttaca tactcttctt caaatacagt cgaattatct
       61 tgtacaatct ctaactttat accttctagc atatcatata acccatccac atataggttt
      121 gatctatttt gtatatattt tcatctatat tatctgttaa ttctatcgtt tgatctattg
      181 atatatcttc aatattttgt acttcctcaa atccatatag tctagttaat atattactta
      241 tatcgtatgg tattaatatg gaattatcta atatatttct aaactccttg taaataaaat
      301 attaaattat ttatttcttc cttatctaat ttacccttaa ttacaaatct ttcatcatct
      361 tcaattaagt cacactttaa ttcatatatg aataaatttt taggttttat tattttatct
      421 gtcatattgt actccttttt atttatctat ttgcttctgc atatattttt tcagccaaat
      481 aatgaatcat ataattttga agattattat tagttaatat ttctttaacc aatctatcat
      541 tcatattctt agtcttaact atacctctct taaaataact ctttccaaaa gtactaacca
      601 aatcttcata actgttagct ttagctatat ccataatact cttatcattt ttaaacatct
      661 cttcatattg atctattata atcctatctt cctctgtaag tcctactcca aagacatcat
      721 ttattttttg gattatatct tctaaatatt cttcatcagc ttcacttaca cgagaagtag
      781 cagtttcaga tataccaact aattcacctt caccattata taatgatata tcactacctt
      841 ttttaccatc ttcacctaac ttttgtaact tataataatc taaacttaaa atatcatcta
      901 tatctattct ttctttatcc tttttaggta gcttccttaa aagtgctact aagtaaacct
      961 gtaaccttaa aagatttaag ttctttatag ggtaaatttg gattaagaaa ttataaaaag
     1021 taatatactt ttgacatttt ttcttaaact catctgctga cttttcatct tcttcatcta
     1081 aataacaata tctttcaact gccttatcaa ttaaactatt catcttagca tcttgtgtat
     1141 ttgttctttt atccttatag aataatactg caaagtcatt tacttcctca tcagtatata
     1201 taccatattc atgtaactga taccaaagat catttactac attaggctct gtaactttat
     1261 ctatgtttgt catttcaaag tatggtttat atgcttcttg aatatcctct acactatttt
     1321 ggaaatccaa gataaatgta tgcttattag gatatgttct attaagcctt gataaagtct
     1381 gaacagtttt tacaccatct aacttcttat caacatacat agcacataac tttggttggt
     1441 caaaaccagt ctgatacttt tctgcaacta taagtaatct aaagtcactt ttttcaaaaa
     1501 tacttggtaa atttgaatca ttaatatcta gtgctacttc cttattcata ccaacttctg
     1561 tatattcaat atcctcatct tttacagtat ctgaaaaagc aactaaggat ttcatattgt
     1621 accctttttc ttttatatat tgatctattg ctaatttata cttaacagca tgtaatctac
     1681 ttgctgtaac tatcattccc ttagctctgc cttctaacca catagaccta ttatccacaa
     1741 aatcgtctac tatagtttca actttctgac gtatgttgtg ttcatttaaa ctaataaatt
     1801 tgtttatagc tcttttagct tctcctttat caaattcggg attatcctct atttttttac
     1861 ctaccttata atatacacta actggcataa agttctttaa tacgtctaat atgaatcctt
     1921 cttctatagc ttgcttcata gaatataaat gaaactcatg aggtagtcca tcttctccta
     1981 ttctaccaaa tacttgcata gtcttattct taggagtagc agtaaatgca aagaaactta
     2041 tatgatctaa attagttctc ttttctataa ggtcattcat cttgtctata ctagattttt
     2101 cattcttttc attttcagca tcaattatct tagcttcttc taatgttttt cctgctaaag
     2161 tttcttttaa tgcattcata ttctcaccag atgtagaaga atgagcttca tctattatta
     2221 ctgcataacg tcttttttca agttgttcta tcttaggtaa tgcatattgg aacttttgta
     2281 tagttgtaat aataatcttt gctctgttta caatagcatc agctaaatct gatgaatttt
     2341 tatcatcatc tatcttaaca actaatccgt ttttgtgttc taattggtat atagcatctt
     2401 gaagttgctt atcaagtacc tttctatctg taactactat tacagaatca aatatagcat
     2461 tgttatcttc atcatgaagg ctcgataatc tatgagcagt ccatgctata gtattagtct
     2521 ttccactacc tgcactatgc tggataagat aatttttacc tgctttattt acttctaaat
     2581 ccgctactat ctttcttact gcatctagct gatgaaatct aggaaatata gctcttttac
     2641 ttttcttatc atcttcttgg ataaagtaaa atctctttat aatatcaagt aaactatctt
     2701 tagtaagtat ttcttcccaa agataagaag ttttttcttt tccttctact acaggatttc
     2761 ccttacccat attgttacct ttattaaaag gtaagaagaa tgttttatct ccttttaact
     2821 ctgttgtcat atatacttcg tcagtatcta ctgcaaaata tacaaatact ctttgattaa
     2881 atccaaataa agtttctttt ggatttctat cctgtttgaa ttgttttata gcattataca
     2941 cattttgatt agtgaattgg tttttaagtt ccattactat aacaggaaat ccgttcaaag
     3001 ataatactat atctaaagta ttgttgtgct ttgaactata ttttaattgt cttgttattt
     3061 tgaatatatt ttgcttataa ttttcgtaag ctgttttgtt agtagttaga tttggtttgt
     3121 tgtagaataa ttggaatttt ccgttcatca ttatatcttc tacaccattt ctaagtacac
     3181 ttataagccc tcttcttttg atgtttgagt tgattaaatc tataaaggct tttttgtaat
     3241 tgcttcccct gttgtatttg aactcatcta gtaattcttt ttgagtaatt tctaaaaatc
     3301 taaataaatc ttctacgaat aagcaatttt caagatcaaa ttcttgtgaa tgaccttgtt
     3361 cgtatccaat tgaaactaaa tgattttcaa tagactcttc taaatgtttc tcttttggta
     3421 acattgctat tcccccattt ctatattgtt aatctatata ttataaagct tatattaatc
     3481 attaactact tacgtttttt accagccaca ccaactttat agccatcatc tcttaaatat
     3541 tttcctatta ttgcaggtat acttcgataa gctcttttgc tatttttcat aattactgca
     3601 tatataattc ttgcttcttt atttgattta acatcaaaat atactaattc ttttttaggt
     3661 tcacgtccat tacgcttatc gctccttttc tgcttatctg tccttttatc tacatattct
     3721 ttgatcaaaa tattattttc atttaaatta tttaagtgtt tttttatatc ataattaact
     3781 ttatcatcaa attctttata ttctttttca cgtatttttt cctttgaatt attttctttt
     3841 acattatttt ctttatttaa atttttatgt ttagatatta tattatttga cttatctata
     3901 atcttttgag attcacttat tttttgcttt ctagctttat ctaaaatagg gtcatataaa
     3961 taagatatta ttaaatcctt agaaagtcta tatccatcta aatgcatatc cataatttca
     4021 gttttcctta tttctattac tgttttacct aaaactttcc attcctcaaa ataatctgct
     4081 gtatttacag atttataaac tgatatctca caatatattt ctctattgtt atcagtgtaa
     4141 agtattatat ctggtgtata ttttttatta tttttttgac ttgtattata aattggaata
     4201 gcatttacaa tattaactct ttctttttgt atatctgctc ttatagtatt ggtatgtaga
     4261 tttttatata atttatatga actctgtata cttaatttaa agccttcttg tgcactttta
     4321 catacataat cttgtatctc tctatgtata aattcttcta attcataata attctgtctt
     4381 agtttcatac ttttagagtc tgttctaaat atacattctt tatttgtctt tttataatgt
     4441 ttcaagtgaa ttggtactat gcttcattgt ctttcaaatg ctgatactat aaacattctt
     4501 tctctgcaat aaggacatat aaaagtatta tatgcactct cctttgtttt tgatattaca
     4561 ctatttctta catcaaaaaa ataacacttc cccgaaattt tttgtatacc atatcttaaa
     4621 tacctattag tagcatttag ttttctatgt tctacaagaa ctccaatttt atcatttata
     4681 tcactattta taggataaag tctcgtatct tcttctattt taaatccatc tttgtatctt
     4741 atatttaatg ttttcatatc aatataatct cctacataaa gcaatgtaaa atataatttt
     4801 attgattata tatattacta aaatatatag ttatttacat acttagttat atctattggt
     4861 tctttatata aatgaactct agcatctata catcttcttc ctataaaatg aggatataat
     4921 tcaaaatcaa tttctccatt tttctttttc ttagtttcta gtacatagtg ttgttttaat
     4981 tcttcttttg taaataccac agctttttct atatcttcat tgcatattat agtataataa
     5041 tcataatctt cttctaattc ttcactagaa aaacttgtcc ataaatatgt attaggatct
     5101 tctccttcgc tataattttt acttgttttt atctttattt ttttgtcacc atctatagtt
     5161 ctaaaaacta tttgatttgc ttttgtttct acgttttcta ttttttctaa gttattttct
     5221 ataaattttg ataaaacttg cattgttata ttttttctac ttaatttcat atttttcacc
     5281 tctgttcaaa tatactaaga gttagtctag tatttctatc tttcctgtca ctgcttcact
     5341 tattaaactt tgtttagctt cttttagttt atctatttgt aatttagttt tatctataat
     5401 agaatctatc cctttacatt tactatctaa atactgagct atattccttt gttctaataa
     5461 atctggtagt attacttttg taccctttaa tattgattga gttatactaa aaactttaac
     5521 ccctgatacc tttgtgcgta tttgtattct ccaatcattt gaatcaaata gatacgacat
     5581 atatctatag tttacttgtt ctaaagttct agctattata gtatggtatc cagcaaacac
     5641 cttattattt tcatttaaat atgtaaaatt accacaccct tctatgtctt ctgatgtatc
     5701 acaaaaaaca aaatctccgt actttaataa tgaaatagaa ttcgatttta aatatgattc
     5761 atctacgtac tttaatttat gtttactagg cttaagctca aacctatatt ttgagtgtat
     5821 ctctccataa tttacacacg gtattccact ttcttttaag tctgcctttg ttattgataa
     5881 acctttattt aaagtaaaca tatacttaaa gttcttcact tcccaatcct taggtatttc
     5941 acctaaccat tcaattccac tatccttcat ttcttcacta cttctcttca caagcttata
     6001 tccatcatca gtcttaacaa ccttcacctt accagtaaca acctcactta taaggctctt
     6061 tttagcttct tctaactttt ttattaattc ttctttctta gatataataa attcaaactg
     6121 tgaagtcttt tcatcaagga aattagctat tttttcttgt tcttctaacc tatttgttat
     6181 tactggaaga tttaaaaata cttcattttt taatgtccat ctatgttcga atgatacccc
     6241 ctctccccac gggaataaga aattatgatg ataatgcatt tgaaaatatc ttttataata
     6301 atcgtgatta atatttactt tatttttact tctaaaggta gtatatgccg gactaattac
     6361 accttcaatt tctgctttat cagtatatcc tgatattaaa tccattggat taagtataaa
     6421 atcatttttt tcaatcttag tgtatcctac atatgttgat gctaattgtc cctcattatt
     6481 acttacatct cttattttta atcctttttg agttaatgat aataccgttg gattttcttc
     6541 atcagattta tctttcttta attcaaataa atgttttatt ctttttactt cccaatcctt
     6601 aggaatctta cctatccact caactccact atctttcatt tcctcatcat ctctatatct
     6661 atatctcatt accctagcac cttccttatc tccagtgcta tctcactttc taaactttca
     6721 acttccttca taatatcatc aaagcttttt aacttttcat acttataaaa atatctagta
     6781 aaaggtatct catatcctat attatctata ctactttcat ctatataagc atcttcaaca
     6841 tgaggtttta cctctttttc aaaatactca tacacatctt ctttaagtgc tatactttca
     6901 gtatctttta aactactatc actctctata ttccctttag catctttgca aactactgcg
     6961 tcttcatttc ttactcctat agcattaact atagctttaa ttagtcctgc tgataaagct
     7021 acatcatata acttagactt agattttaaa tctttaataa acttattcct cttcatatac
     7081 tcaaattcac tatcaaaact ttctaaaagt tttaataact tatcttgttt tactcttcct
     7141 tctgcttctt ccttaacttt aacttcttca tcttttttct tagaaactga aagatttata
     7201 aattgagtag tatttttaac attttctata gcttcttcat ttactctaaa gctcagtttt
     7261 aaaggtcttt ctattgttac ttttctatat ccaaagcctt cattgtcaaa tattttacta
     7321 ttttcacttt cttcaaatct ttcatatatt tcttttattt cttctatttg ttcaagactt
     7381 atttcttttc ttttatttcc taaacttttt ctcattaatt gataatattc actagcattt
     7441 acaagttgta tcttaccttt tcttttatcc tcttttttat tagtaagtac ccatatatat
     7501 gtagctattc ctgtgttata gaacatatca gtaggtagtg ctattatacc ctctagtaaa
     7561 tcattttcta ttatccattt tctaatgtta gattcaccac ttcctgcatc tcctgtaaat
     7621 aatggtgatc cattgaatat tatagctatt ctactacctt cttcatcatc atacattttg
     7681 cttatcatat tttgtaagaa tagtaacgac ccatcactta ctcttggtgt tcctgcccca
     7741 aatcttccat caaatcctaa gtcagcttct tcttttactt tcttctcttc atctttccat
     7801 gtgacaccaa atggtggatt tgatattaaa aatcttatct tttctccact aaatctatct
     7861 tgcgtaagag tatttcctag tcttatatta tctggatttt gtcctttcat tatcatatct
     7921 gcctgacata ttgcaaatga ttcatctagt aattcttgtc catatacatt tactatggca
     7981 cttggattta ctttttccac atagtcttca gctattgaaa gcatcccacc agtaccacag
     8041 caaaaatctg ctatagatat tacttttcct tcttctgtaa ggaagttttc tttgcccata
     8101 aataacattt ccatacaaag tcttataact tctcttggag tatattgttc tcctgctgct
     8161 gagttttctg taaatcttct tagcatttct tcgtatatgt atcccatttc gttgtttgat
     8221 acagagttta tatgtaggtc tacttcatac attttgctaa ggactgcata tagcttgtct
     8281 tttttatcta attgagctat ttcatctttg aatttaaatt tccctattat ttcttttaca
     8341 ttgtttgaga atccatttag gtattcttca aagttcgatt ttatattttc actatcatct
     8401 aacagttttt tccatgtaaa atcactcaca ttgtagaact gtaagtctat attatggttt
     8461 tcttttagat ccactattac aagttcgtct ttttcttctt ctggtaagaa atcatattct
     8521 ttgtatactg atttcactat ttctctatca taatcatcta atagacaatc aaatcttctt
     8581 attactatta gtggtaacat tacttttctg tagtcttctt ctttgtatgt accccttaaa
     8641 ctttctgcta tattccataa aaaagcaata tgattaaaat tattatctat actcacttat
     8701 actatctcct tttaattaaa ttttaaatta gtttatatta taattatatc atttcatggc
     8761 acaaaaaaaa catattgaca aaactatcaa tatgtttttc gtggatttca atggtggaga
     8821 cgaggggatg ctacattctt atttttacat cttcatgtct catcactttt tgcgttatat
     8881 caacacttat aatttttata tttcatgtat atttttataa gttcatgtat gttattaaat
     8941 tagttgtgtg ctaaatgtgt gctaaattca tatattattc attaataaag tggtatcgtc
     9001 caaaacgata cctaataaat ataatatctt ataaataata agcctatcat tatactctat
     9061 ttatttatgt agaatcgttc gacaaattac tgatataagt tcttaaatat gctataaatt
     9121 aaagtatatt agacattaat ttatggaggt ataaaatatg tttctaaatg atatatttga
     9181 tattattaaa tttaataaaa aaagccctat agaacagtta tatgttattc aagaagaaga
     9241 aaagccagat ataaaagaag ctgaggaata ttatagatgc aattacagat tttataattc
     9301 tcttaaagag atggattcaa tagaaaatat aaaaacagaa aaaacacgca tcaaaactgt
     9361 cttagattct aaaaacaatc catcaattcc atttgcaatt gctattattg ctgcatttat
     9421 atcaattttc tttagtctac ttactaacaa tattataaaa gattttttta ctgcttgttc
     9481 tgtaatagga gttttttttg gtacttattt atgtttaata tgtgaatctc agcaaaaatg
     9541 ggatagtcgc caaaaattct atatgagttc gctacaagta ttagataata tggaaagtaa
     9601 aaaacttaaa gaaagaaagt acaaaaaaag aaggtagcaa ctaaccctag ctgctacctt
     9661 ctaatctatc tatcaataaa atccaatgcc ttataaagag tatcaaatct atcattacct
     9721 tttatcattg tatagtgttc tttagtaata gtccctatct tctcacatgc tgcaccacca
     9781 atgacataaa gattttgcgt ctgacctggt acgtaatctt ttatatcaca tatcagtatt
     9841 ttcccatcat tataacccca accaactaca gttgcaggga ttttgtcaac ttctccatca
     9901 taaacaattg tatgtttgta catctgttta actccctcac tatttatatt tttatttaat
     9961 acaccttcta caattaactt agcaatacct tcatgaccta gtttcttagc tttctcataa
    10021 tcttctttat tgtcacaaaa gaaactttca attagtactg ctgtaggttt ggaactattt
    10081 aaaatatata atcctttatc tagtttagca cctctatttt taaatactgt gcctagtttc
    10141 ttacatattc tagttgcata ctctaaaccc ttattactat aatatagcac ctctgagcct
    10201 tttccttgcc catcacttgc atttaaatgt agttctatga gtaagtcata gcctccacta
    10261 ttaacctttg gtattttgta agtcttttct tcactcttag ttttaaactg cttttcgggg
    10321 catattatta tatctacctt atgtccttct ttcctaaaca catctgctaa tactggtgca
    10381 agagatttgt tatattgata ctcgttaact actccatcag cagaagtaca tgctccactt
    10441 tttaaaatac tatgccctac tgtaatacat attttcatta tttattttcc tccttcaact
    10501 gtttgtaagt ttgatttata cctattgata taccccaaca aatcactcct tgtaagactg
    10561 cactaggatt taatcctaac atccacaccg agaaacctac accaagtgtt aataatataa
    10621 ctggaatata cttgttatct aattgcttat atttcttgca acctgctcct ataacataaa
    10681 gagcagcaac taaaattagt aactgctctg gtataaaact tattaaatta tccatctttt
    10741 atcctcctaa ttaattaaaa tattcctttc tgtatggcaa atataaagaa ccctactaag
    10801 gttgtaatca tagttccaat tagccacttt agcatacttg taagtgagtt taagttctca
    10861 cacaatgctt ttaactctgc tttagactct atatttgcta tctttaattc gtctatttct
    10921 tctccatgct tatttattct tgtttcatgt ctttttaaat ctgcttcgaa aagttcttca
    10981 ttcatgtaaa cctccttatt tttgaattaa aaaagaacta cgctatatag tcctctccta
    11041 ctatttcttt atgttttatt ttattgcata ccatgtaact ccaagtggtg ctgttgatac
    11101 attgtatggt ggattcatta ctatactgta aattgaattt tcatttaaaa ttttaaatga
    11161 tatagaattt attctacctt ctaacaataa aacgccttct ttataatatg ttgctgtgtt
    11221 atacctagaa taatcaattt ctccaataaa tgcaattagg atttcgggtt caaaatctat
    11281 ttctaaatta gcctttttct gctccatagt taattgtata tttcccattt taaacttctt
    11341 tctatcccca ataagacttg ccaattctgc gaaagtattg tcttttgtag ctggtgagcc
    11401 gatagcagtt gcaattagct cttttccact atcggcactt tgaaaagcca aatctgctct
    11461 atctatacat tctttcaatg ctccttctac attatcactt gtaaaattat tctctgtatc
    11521 ttctatagtt acattctttg cttctaatac aagatttcta actttattaa ctaactcctt
    11581 aaaagtcatt taatcacctt ctttcaataa aaaaagaacc ctctatatag ttggttctgc
    11641 tccttctact actccacttt ctctaattat ataatcctct acagcttttc tgtactctac
    11701 attagttaca tcatctaatt caaattctcg attttttaga gggtttaatc ctccacctaa
    11761 aattctctct gctaatattc ttaccacaac attatttata ttcattataa aattccccct
    11821 actttttcat tttctgcaat taatatttga ttttctaact cttgtattct cttttcttct
    11881 tctgtaacga atattggtat ttcttccaaa attggttctt tcgtttctat atttatacct
    11941 acaatcctat tttgcatata atctatactt ccgtatggaa tatcaatgca atgtaattta
    12001 gttattgtat catgttctaa tatatctcct gttgcttctc ctgtttggag aagtattttg
    12061 cctgtttggt cgtaaattat tctatttgct ctattcattt tatcacccca tttatacaaa
    12121 ttttatagca taccaattgt acaaagtata agacacagaa ggtctataag caggaacata
    12181 gacaccttgg ttatttatat aagtattacc tctattattt atataaacct gactatcagc
    12241 tgtaaatttg gtatctgaat ttgtttttct aagagcggtt gtaattacaa aatctttatc
    12301 agaaagtgat gggattaaat aggtggcaaa gattaagtat ttataaaatt gtgttgggct
    12361 attagtcgta cactcgcatt cagcaaaaaa gatatttgga ataaaattta atcctttaat
    12421 attaacccac cctcctgggt ttattggata attaggatta gactggctat taacatataa
    12481 ataagcagtc gatgttgtgt ctgttaatgt actggtacgc cctgatgcaa ctttatattt
    12541 tgtatttaac tgtgatatag tattgttagc ttgtgtcaac tgattcatca aatcctgcac
    12601 actagcgtct gaactatcaa aacttatttt tattttctct gataactcga ttaatgtatt
    12661 atttaaactt gcttctatat tctttaatgc taaagtattt ataatgcttg ttttgccact
    12721 tttaaatcct gcgttaactt ctgttaattt tgttgatata tcttgcaaat tcacattctc
    12781 aggtagtggc attatattct tacttatact taatactttt tctgctgtag cattattact
    12841 gtctgtaacg actatcttaa gtgtgtgtag tgcattatct tctagtgtat agttaattgt
    12901 tttttcaaga gttaaatctg ttgttatagt ttcttttaat gtatcatcta taaatacttc
    12961 tatcttagtt agtag
//
LOCUS       DAEPXF010000104        12795 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain HB27
            SAMN13376389-rid12479423.denovo.110, whole genome shotgun sequence.
ACCESSION   DAEPXF010000104 DAEPXF010000000
VERSION     DAEPXF010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13376389
            Sequence Read Archive: SRR10518432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 12795)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12795)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001069624.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001069624.1
            Genome Coverage       :: 315x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/15/2021 01:54:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,165
            CDSs (total)                      :: 4,076
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 89
            rRNAs                             :: 10, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 10 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 19 of 69
            Pseudo Genes (incomplete)         :: 44 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 10 of 69
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12795
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13376389-rid12479423.denovo.110"
                     /strain="HB27"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="China: Hebei"
                     /collection_date="2017"
     gene            131..319
                     /locus_tag="KRL67_002543"
     CDS             131..319
                     /locus_tag="KRL67_002543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HBH1523411.1"
                     /translation="MAKVCSVCGKGKVSGNQVSHSNKHNKRTWSANLRSVRAIIDGAP
                     KRVKVCTRCLRSGKIERA"
     gene            complement(479..631)
                     /locus_tag="KRL67_002544"
     CDS             complement(479..631)
                     /locus_tag="KRL67_002544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate translocator"
                     /protein_id="HBH1523412.1"
                     /translation="MRNNKSKVLLGILCLATGMAVILSMLLPGWIWSALTALILIGCG
                     ALLFFC"
     gene            complement(729..1205)
                     /locus_tag="KRL67_002545"
     CDS             complement(729..1205)
                     /locus_tag="KRL67_002545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HBH1523413.1"
                     /translation="MNLKRSNEKREEYRRMYSDKESFLSLIQNFTSVSIAKELTLKNI
                     ELETSFICEYKGKEVDIIYKVFSKSGKVSHYIVLEFQTEMDTEIVPRLKSYREQIWKS
                     FIMKKSLEEIEDKNFKLPKVIPVVLYSGPERLSTKKGILDIVESISDFSNNSSMKN"
     gene            1424..2185
                     /locus_tag="KRL67_002546"
     CDS             1424..2185
                     /locus_tag="KRL67_002546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HBH1523414.1"
                     /translation="MEYTVQKLSKLAGISTRTLRYYDEIGLLKPLKINSSGYRIYGQN
                     EVNKLQQILFYRELGINLENIKNIINSPNFDSLSALKEHHNKLLVKRKQIDLLIENVT
                     KTIALKEGKYTMTDIEKFEGFKEKMIDENEKNYGTEIREKYGKDIIVASNKKLKNMSK
                     QDYEDWQNLSIEIISKLKKAFKTGDASSELAQEVARLHHKWLSYTWSTYSKEAHASLA
                     QMYVCDERFTSYYDKEQPGLTKFLRDAIVVYTRDS"
     gene            complement(2444..4372)
                     /locus_tag="KRL67_002547"
     CDS             complement(2444..4372)
                     /locus_tag="KRL67_002547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HBH1523415.1"
                     /translation="MHKRLLTLFKLLNESDDKITCKALSNHLKVSERTIRNDITSING
                     TLEKNGAIIKIKKGEGYYIDILNLALYQQYLALISDDIMDSSEIPDSPIERNQYILKY
                     ILYNNTYIKLEDLANSLYVSKFTILNDIKRIKPILLKYNLILVSKPYYGVKIEGKEID
                     IRRCISNNMINRNFENYIIGITDREIELFNNIDLIELQKVVLSEINKFNINFLDFNLK
                     NFIIHLAITISRILDGYCLDNVLDVVLTDFQSNTTVENIFNYIESKYTIIISKADRVY
                     LYNHFITKSSLLDNVSNRVDTKIIEYVEEILEVINNQYTFDLRNDSVLFDDLVLHFKS
                     ILNSKSYNLNKVNPLINTIKSNYPLAFEITLNAIEKVFKNSIYSLTEDEIGYVSLHIG
                     AGIERFFQNNIKCKNVVLVCGSGYGSSRLLEVQLNKVFHDKINILQCLSFNQFLASEL
                     SDVDIIISTIPLNHDSIPIVLVDLKLLKKDIENISKSITNNSHIYNNLLDNFFDKNLF
                     IVNPKIKDKDELIKLMCNKLTQSEIVIPSFTESVFYRESLSSTNIDDFLAIPHPMELS
                     SIKTKICISILNEPIYWSEDSTVKLIMMLAINKDDYIKINSIYDILLKIIHDNDIRDS
                     ISNCNDFDSFLSIIKSII"
     gene            4607..5059
                     /locus_tag="KRL67_002548"
     CDS             4607..5059
                     /locus_tag="KRL67_002548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBH1523416.1"
                     /translation="MVLQEKNIFIDKNLKSKEEVLNFIADSAYILGITTNKEALLKDL
                     LKRENEFPTGIKDGFAIPHTKSVNVKEASILYIKTTNELEWKTLDDSKVRYVFSLLAP
                     HENEGNIHLKTLSKLATCLMEDDFISEVKNASDASHLVKYITNKMKEE"
     gene            5062..5370
                     /locus_tag="KRL67_002549"
     CDS             5062..5370
                     /locus_tag="KRL67_002549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HBH1523417.1"
                     /translation="MKIVAITSCIAGLAHTPMAAKALEQAASKMGHKIKVEQQGAMGR
                     VNTITKEEAQDADFILIASDQTISGMERFDGKKVIKVKIGIALKKPEAVLTKCIEAIS
                     "
     gene            5487..6593
                     /locus_tag="KRL67_002550"
     CDS             5487..6593
                     /locus_tag="KRL67_002550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HBH1523418.1"
                     /translation="MSKKNKSLLSELKGHLMTGISYILPLIIGSSLVVAIPKLIALAM
                     GITSLDAYKNISGFYHILYLMEQVGWTGIGLLNTVLAGFIAFSIGEKPAMGAGFIGGL
                     IASNTNAGFLGAVIAGFFAGYVCKFLKKKIKISGSAAGMMPLIIMPLITVGLTGFLMS
                     VVLAGPLGNINTSLNAWVAEMCQNGTNSVVLALILGAMIGFDLGGPVNKAAWMAGNAL
                     LLEGIYLPAILVNCAIVIPPLGYGLATFIRKSKFSNALAETGKGNIVMGIIGITEGAI
                     PFTLTNPLKLVPVNMIGCALGVGLSALLGVHAIMPPVGGLYGFISIGSGWAYLVGAIF
                     GALVIAILSTTLVDFNEDETTENTEFDEIELDIM"
     gene            6668..9346
                     /gene="mngB"
                     /locus_tag="KRL67_002551"
     CDS             6668..9346
                     /gene="mngB"
                     /locus_tag="KRL67_002551"
                     /EC_number="3.2.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosylglycerate hydrolase"
                     /protein_id="HBH1523419.1"
                     /translation="MKKTKVHVVPHSHWDREWYFTTSRSKIYLMKDFQDILDILEEKE
                     DFKYFTMDAQASLLDDYLKWRPQDEYRIRDLVQKRKLVIGPWYTQTDQLVISGESIVR
                     NLYYGIDRCNEFGEPMRVGYVPDSFGQSAQMPQIYRGFGIDSSLFWRGVSDDMVDTTE
                     FIWKGSDGSKVLAVQIPFGYYYGGNIPEDDSDLKEYLKDTIGKLKVRASTNNVYFPNG
                     FDQAPIRKNLPDILKKANEIDNENEYEISDIENYIDSVRNERNEFTELKGEFLNAKHM
                     RIHKSIFSSRSDLKIMNNKLENYITNVLEPLLTLSYSLGNEYPHLVVKDIWRLMFENT
                     AHDSIGSCNSDTTNEDVYTRYKQARDLSINLLDLHMRLISTQIKNNNEEITLTVFNGL
                     PEVRNEVVEFETYIPGQDFVIKNKNGEILNYIIKEKEDISDYVLTQTIRLNPSKKIYI
                     PKNVYRAKIALSVKDIPPMGYTQLVFELDTKNKCKVKTSNFKEIENKYYKITANKNGS
                     VEILDKESNKTYKNQAVIEDNGDDGDSYNYSPPRKDIYVSSLDSVSSVEVLKSNIQEE
                     ILLKYSLTIPASLEERAIGKVSVKMPVTMKITLEPKEQIIKFNVNIKNDQALSHRVRV
                     LFNTEIASKFSIADQQFGVIQRPTVLEKDLVLWKRDNYSWQEKPITIEPMQSFVTLEN
                     GQRGIALITGCVREYQIVGDNLDTIALTLFRSFGYMGRENLLYRPGRASGEKIIATPD
                     AQLLKELNFDFGLYIYNNKFDEANVANTAKRFLTPIQIYEYADFLNGRLIFAFNDEKQ
                     IYENEYSLMNVNNSNFTVSAIKKEEKGDGVVVRIFNGMKNEDAKGSLNINKNINGAYS
                     AMLDEIYDNTNKLKVNTKTVEVNSLSHCKVQTIVIK"
     gene            complement(9668..12001)
                     /locus_tag="KRL67_002552"
     CDS             complement(9668..12001)
                     /locus_tag="KRL67_002552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HBH1523420.1"
                     /translation="MDTKLKKFKDKYIVTIIVFILMLSASLGMISQYSTIQAGAKGGA
                     KNPFEQESFVDSIYKGSYVLYHNMKEEQEGKMIQPSKLFINEETMNYIKNVSKEKDTY
                     DGSETYYTDDSINADFNENFESWESFIKESRKNLKYYLVDKESKFEFFNDNKELGALD
                     TTKNDVSKEDSDENQSKNTEMNDEERQKQITDIKENYRFYVVMNFDKDGKVNVVDSYG
                     VNQKVINQMLLSKSREDLMDIDLGGNTISYKLSPIKNMTLIYAVPKVISKGDSREYSY
                     GNMYSNDNISSYINNVENNSYYEISRMVLKIILAIVVIVALVFPYRKSKELLGFDTLS
                     RIPLEILFIILAIIQGIVDLYPTGIISDTLNGNYVEYLINNDISAESASILVSLMNLT
                     YWFVIFSIIFVFITLLKHILNVGIREYFIKNTVTGIFFVWLKKAGKTVINQIRTINLK
                     EKPNKTIVIILGINLLIIVIMCSMWFFGIILALIYSAVLFKILSDYSKKTICEYNQLL
                     DVTKKISDGNLEVNMEKDLGFFNPIKEELGNIQTGFKKAVDEEVKSQKMKTELISNVS
                     HDLKTPLTSIITYIDLLKDESITDENRKLYIDTLDRKSQRLQHLIEDLFEVSKANSGD
                     VHLNIVNVDIVSLMKQTLLELDDKLADSSLILKNNFSSEKIILPLDSQRTFRVFENLI
                     INISKYAMPNSRVYIDILETDNRVNIMLKNMSATEIDFSVDDIMERFVRGDKSRNTEG
                     SGLGLAIAKSFIELQGGKMNISVDGDLFKVTITFNKN"
     gene            complement(11976..12692)
                     /locus_tag="KRL67_002553"
     CDS             complement(11976..12692)
                     /locus_tag="KRL67_002553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBH1523421.1"
                     /translation="MNSYNILVVEDEKEIADAIEIYLLNQGYNVFKGYNGLEGLKVIE
                     NQEIHLAIVDIMMPKMDGITLTMKLRENYNFPVIMLSAKSEEVDKIMGLNIGADDYVT
                     KPFKPLELLARVNSQLRRYTKYLNMVGNKEQKVESDDSVFAIGGLELNENTKEVSIDG
                     KYIKTTPIEFKILSLLMRNAGRVFSADEIYERVWNENAVNTDTVMVHVRNIREKIEID
                     PKNPKYLKVVWGVGYKIEKI"
BASE COUNT         4770 a         1775 c         1668 g         4582 t
ORIGIN      
        1 tatgataata agaaattctt caatatgtgt aaggcaaata tatatttcta ttgcaaattt
       61 ttgttattta tgataatata aatatgtttt tagtctttaa atagttatca ttttaaggag
      121 gtgtgcgtag atggcaaaag tatgtagcgt atgtggtaaa ggtaaggttt ctggaaacca
      181 agtatcacac tcaaataaac ataataaaag aacatggtca gctaacttaa gaagcgtaag
      241 agcgattata gatggagctc ctaagagagt aaaagtttgt actagatgtt tacgttctgg
      301 taaaattgaa agagcttagt aaccacgttt aaaagcctac ttccgtaagt aggcttttta
      361 atttaatcat ttttactctt tctcataaat tttaggatta tacttgaaat ccattttggt
      421 aattttattg ttactacttt catcaatact caccccacac tttatagtta aaactatttt
      481 aacaaaaaaa taataatgct ccacatccaa taagtattaa tgcggtaagt gctgaccaaa
      541 tccagccagg aagcaacatt gacagaatta cagccattcc tgttgctaga caaagtattc
      601 ctagcaatac cttagacttg ttatttctca tggaacacct ccacatagaa cctgtatcta
      661 taaatatttt catattcaag tttatgcgac agttttccat tttagaccaa taaacatatt
      721 atgtaatttt aatttttcat actagaatta ttactgaaat ctgatataga ttctacaata
      781 tccaaaattc cttttttcgt gcttaacctt tcaggtccac tatataaaac cacaggtata
      841 actttaggaa gtttaaagtt tttatcttct atttcttcaa gacttttttt cataataaaa
      901 ctcttccaaa tttgttctct atatgatttc aatcttggga caatttcagt atccatttct
      961 gtttgaaact ctaacactat ataatgtgat actttaccac ttttagaaaa caccttataa
     1021 attatatcaa cttcttttcc tttatattca catataaaag atgtttctaa ctctatattt
     1081 ttaagggtta attcttttgc tattgagaca cttgtaaaat tttgaatcaa actcaaaaaa
     1141 ctttctttat ctgaatacat tcttctatac tcttctcgtt tttcattgct tctttttaaa
     1201 ttcatacagt tcctcctaga ctaaagactt tctatatttt aataaagaca gtctataaat
     1261 tttatttttt aattttcact agtgagtatt gacacttgtg tgtaaaatat ccagtaaaga
     1321 tattttttta tatttttatt aaaagatata aatttttata ttttaatata ttgacattta
     1381 cgttgcgtaa aagtgtatat ttaatttata ggaggtacta cttatggaat atacagttca
     1441 aaaattaagt aaactagctg gtataagtac tagaacactt agatattatg atgaaatagg
     1501 tcttcttaag ccacttaaaa taaattcttc aggttatcgt atatatggtc aaaatgaagt
     1561 taataaactt caacaaatac ttttttatag agaattagga attaacttag aaaatataaa
     1621 aaacattata aattcaccaa acttcgatag tttaagtgcg ttaaaagaac accataataa
     1681 attactagtc aaaagaaagc aaattgattt actaatagaa aatgtaacta aaactatagc
     1741 tctgaaggag ggaaaataca ctatgacaga tatagaaaaa tttgaaggtt ttaaagaaaa
     1801 gatgattgat gaaaatgaaa aaaattatgg aactgaaatt agagagaagt atggtaaaga
     1861 tattatagtt gcctcaaata aaaaacttaa aaatatgtct aaacaagatt atgaagattg
     1921 gcaaaatcta agcattgaaa taattagtaa gcttaaaaaa gcttttaaaa ctggtgatgc
     1981 ctccagtgaa cttgctcaag aagttgcaag attacatcat aagtggttga gctacacttg
     2041 gagcacatat tctaaggaag ctcatgcctc tttagcacag atgtatgtat gtgacgaacg
     2101 ctttacatct tattatgata aagaacagcc tggactaact aagtttctga gggatgctat
     2161 agtagtatat acaagagata gttaatataa aataataaag aggatgtctc aaaatgaact
     2221 ttttagttca tgagacactc ttttttatat gaaatcaaat atgtttttat catttcaaca
     2281 ataaaaaaag gattatctct attttgagac agaccctcta ttgcatctaa ttacaatcag
     2341 tataaagtaa tagaaaataa tataaaaatt actcattact atttatatta tcaacaaaaa
     2401 attgttctat tgatttttta taaaacttat gtaatataaa attttatatt atagacttaa
     2461 ttatggaaag aaaactatca aaatcattac aattagatat actatctcta atatcattgt
     2521 catgaattat ctttaaaagg atatcataaa tggagtttat ttttatataa tcatctttat
     2581 ttatagccaa catcatgatt aatttaacag tagaatcttc actccaatat attggttcat
     2641 tcaatatgga aatacaaatc ttagttttta tagagcttaa ttccatagga tgaggtatag
     2701 ctaaaaaatc atctatattg gtagaagata aactttctct gtagaataca gattcagtaa
     2761 atgatggaat tacaatttca ctttgagtta atttgttgca catcaacttt ataagctcat
     2821 ctttatcttt gattttagga ttaacaataa ataaattttt atcaaagaaa ttatccaaaa
     2881 gattattata aatatgagaa ttattagtga ttgacttaga tatattctct atatcttttt
     2941 ttaatagttt taaatctact aatacaatag gaatactatc atgatttaaa ggaattgtcg
     3001 atattattat atctacatct gacaattcac tagctaaaaa ttgattaaat gatagacatt
     3061 gaagtatatt tattttatca tgaaaaactt tgtttaattg aacctctaat aacctacttg
     3121 aaccataccc tgaaccacat actaatacta cgtttttaca cttaatattg ttttgaaaaa
     3181 atctctctat tccagcacct atatgcaggg atacatatcc aatttcgtct tctgttaaag
     3241 agtaaatact atttttaaaa actttttcta tagcatttaa agttatttca aatgctaaag
     3301 gataattact ctttattgta tttataaggg gattcacctt atttaaatta taagatttag
     3361 aattaagaat tgatttaaag tgcagtacta aatcatcaaa taatacacta tcatttctaa
     3421 ggtcaaaagt atattgattg ttgattactt ctaaaatttc ttcaacatac tctataattt
     3481 tagtatccac tctattggaa acgttatcca aaagagaact tttagttata aaatgattat
     3541 atagataaac tctatctgcc tttgaaataa ttatagtata tttactctca atataattaa
     3601 atatattttc aacagttgta ttcgattgaa aatctgttaa aacaacatct aaaacattat
     3661 ctaagcagta accatcaagt attctactta tagtaatagc taaatggata ataaagtttt
     3721 ttagattaaa atctaggaaa tttatattaa atttattaat ttcacttaac actaccttct
     3781 gaagctctat taaatctata ttattaaata attctatttc tctatctgta attccaatta
     3841 tatagttttc aaagttccta tttatcatat tattagaaat gcatcttcga atatctattt
     3901 ccttcccttc aattttaact ccataatatg gcttagaaac taatattaaa ttatacttta
     3961 acaaaatagg ctttattctt tttatatcat ttagtattgt aaatttactc acatacaagc
     4021 tattagctaa atcttctagt tttatatatg tattgttata aagaatatat tttaaaatat
     4081 attgatttct ttctatagga gaatctggta tttcacttga atccataata tcatcagata
     4141 ttaatgctag atactgttgg tataatgcta aattaagaat atctatataa tatccttctc
     4201 ctttttttat tttaataata gcaccatttt tctctaaagt tccatttata gaagtaatat
     4261 catttcttat agttctctca ctcactttta aatgattact taaagcttta caagtaattt
     4321 tatcatcact ttcatttaat agtttaaaca acgtgagcaa tctcttgtgc ataaatatcc
     4381 ccccttatag aatttcttct aaattatact atttacactc atataataca attactctaa
     4441 taaaattgta ttataataat ttatttccat ttcgacatgg aaattagtta ttatcataat
     4501 ataaatttcc atgtcgaagt ggaaattagt tatatttaat ttaattagga taacgattac
     4561 aatataaata taagttaaga cattacgaat ggagtggaaa aaatatatgg tactacaaga
     4621 aaaaaatata tttatagata aaaacttaaa aagcaaagag gaagtattaa attttattgc
     4681 agatagtgct tatatactag gtattacaac taataaagag gctcttttaa aagaccttct
     4741 aaaaagagaa aatgaatttc caacaggaat aaaagatgga tttgcgatac ctcatacaaa
     4801 aagtgtaaat gtaaaggaag cttctatact ctatattaaa acaactaacg agttagagtg
     4861 gaaaacttta gatgatagta aggttagata tgtatttagt ttgttagctc cacatgaaaa
     4921 tgaaggaaat atacatttga aaactctatc taaattagcc acatgcttaa tggaagatga
     4981 ttttataagt gaagttaaaa atgctagtga tgcaagtcat ttagtaaaat atataactaa
     5041 taaaatgaag gaggaataat tatgaaaata gtagcaataa catcatgtat agcaggattg
     5101 gcacacacac ctatggcagc aaaagcttta gaacaagctg catcaaaaat ggggcataaa
     5161 atcaaagtag aacaacaagg tgctatggga cgagtaaata ctataacaaa agaagaagca
     5221 caagatgcgg actttatact tatagcatct gaccaaacta tatctggaat ggaaagattt
     5281 gatggtaaaa aagtgataaa agtcaagata ggtatagcgt taaaaaaacc agaagctgtc
     5341 ttaacaaagt gtatagaagc tatttcttaa taaaaaaaat ctcactctaa tatagaatga
     5401 gaaaatttaa aaatttaaaa attgtctaat gtaattataa catattatca aaaaaatata
     5461 acaaacttat ataggaggaa ttaaaaatga gtaaaaaaaa taaatctctt ttaagtgaac
     5521 ttaaaggaca cttaatgaca ggtatttcat atatattacc acttataata ggctcatctt
     5581 tagttgtagc tataccaaaa cttatagctt tagcaatggg tataacaagt ttagatgctt
     5641 ataaaaatat atcaggtttt tatcatattc tttacttaat ggaacaagtt ggttggactg
     5701 gaataggtct gttaaacaca gtattggcag ggtttatagc attttcaata ggtgaaaaac
     5761 cagctatggg cgcaggtttc attggtgggc tcatagcaag taatactaat gcaggattct
     5821 taggagcagt aatagcaggt ttctttgctg gatatgtttg taaattttta aagaaaaaaa
     5881 ttaaaatatc tggttcagca gcaggaatga tgccacttat aataatgcca cttataacag
     5941 ttggactaac tggttttttg atgagtgttg tattagcagg acctttagga aatataaata
     6001 cttcattaaa tgcttgggta gctgaaatgt gtcaaaatgg aacaaattca gtagtattgg
     6061 ctttaatttt aggtgcaatg ataggattcg atttaggtgg accagtaaat aaagcagctt
     6121 ggatggcagg aaatgcatta ttgttagaag gtatctatct accagctata ttagtaaact
     6181 gtgcaatagt catacctcca ctaggatatg gtttagctac attcataaga aaatcaaaat
     6241 tttctaatgc attagctgaa acaggaaaag gcaatatagt tatgggtata attggaataa
     6301 cagaaggtgc aattcctttc actttaacaa atccattaaa attagttcct gttaatatga
     6361 taggatgtgc tttgggtgta ggattatcag ctttgttagg tgtacatgct ataatgcctc
     6421 cagttggtgg attatatgga tttatatcaa taggttctgg atgggcttac ttagtaggtg
     6481 caatatttgg agctttagta atagcaatat tatcaacaac tttagtagac ttcaatgaag
     6541 atgaaactac ggaaaataca gaatttgatg aaatagagtt agatataatg taaattaaaa
     6601 acttaatttt atgtaattga gaattgagtg tactctcaat tctcaattac attttgggag
     6661 gatttttatg aagaagacaa aagtacatgt agttccacac agtcattggg atagagaatg
     6721 gtattttaca acatcaaggt caaaaatata tctaatgaaa gattttcaag atatattaga
     6781 catattggaa gaaaaagaag actttaaata ctttactatg gatgcacaag cttctctctt
     6841 agatgattat ttaaaatgga gaccccagga tgaatataga ataagagatt tagttcaaaa
     6901 aagaaaatta gttataggac cttggtatac tcaaacagac caattggtta tatcaggaga
     6961 atctatagtt agaaaccttt actacggaat agatagatgc aatgaatttg gtgaacctat
     7021 gagggttgga tatgtaccag attcttttgg gcaatcagct caaatgccac aaatatatag
     7081 aggttttgga atagatagtt cattattttg gcgtggagtt tctgatgata tggtggatac
     7141 aacagaattt atatggaaag gttctgatgg ttcaaaagta ttagcagttc aaataccatt
     7201 tggttattac tatggaggta atataccaga agatgattca gatttaaaag aatatttaaa
     7261 agatacaatt ggaaaactta aggttagagc ttctactaat aacgtatatt ttccaaatgg
     7321 atttgaccaa gcaccaataa gaaaaaacct tcctgatata ttaaaaaaag ctaatgaaat
     7381 agataatgaa aatgaatacg aaataagtga tatagaaaac tatatagatt cagtaagaaa
     7441 tgaaagaaat gaatttacag agcttaaagg tgaattttta aatgctaagc atatgagaat
     7501 acacaaatca atattctcct caaggtcaga ccttaagata atgaataaca aattagaaaa
     7561 ttatattaca aatgtacttg aaccattgtt aacactaagt tattcacttg gaaatgaata
     7621 ccctcaccta gttgtaaagg atatatggag gcttatgttt gaaaatacag ctcatgacag
     7681 cataggttct tgtaatagtg acacaacaaa tgaagatgta tacacaagat ataaacaagc
     7741 cagagatttg agtataaatt tattagattt gcatatgaga cttataagta ctcaaatcaa
     7801 aaataataat gaagaaataa cactaacagt attcaatgga ttaccagaag tcagaaatga
     7861 agttgtagaa tttgaaactt atattccagg tcaagatttt gtaataaaga ataaaaacgg
     7921 agaaattctt aattatataa taaaagaaaa agaagatatt tcagattatg tattaactca
     7981 aacaataaga ttaaacccaa gtaaaaaaat atatatacca aaaaacgtat atagagcaaa
     8041 aattgcactt tcagtaaaag atataccacc aatgggatat actcaactag tgtttgaatt
     8101 ggatactaaa aataaatgta aagtaaaaac aagtaatttc aaagaaatag aaaataagta
     8161 ctacaaaata acagccaata aaaatggttc agtagaaata ttagataaag aatctaataa
     8221 gacttataaa aaccaagctg taattgaaga caatggagat gatggagatt catacaacta
     8281 ttctcctcca agaaaagata tttatgtttc atctttagat tctgtatctt cagtagaagt
     8341 gctaaaatca aatattcaag aagaaattct acttaaatat tcattgacta taccagcttc
     8401 actggaagaa agggccatag gtaaagtttc tgtgaaaatg ccagtaacaa tgaaaattac
     8461 cttagaacct aaagaacaaa taattaagtt taatgttaat ataaaaaatg accaagcact
     8521 atctcatagg gtaagagttt tatttaatac agaaatagca tctaagtttt ctatagcaga
     8581 tcaacaattt ggggtaatac aaagaccaac agtattagaa aaagaccttg tattatggaa
     8641 aagagataat tactcatggc aagaaaaacc tataactatc gaaccaatgc aaagttttgt
     8701 tactttagag aatggacaaa gaggaatagc attaataact ggttgtgtaa gagaatatca
     8761 aatagttggt gataatttag atacaatagc attaacacta ttcaggtcat ttggatatat
     8821 gggaagagaa aatttacttt atagaccagg aagagcttct ggagaaaaaa taatagcaac
     8881 accagatgca caattactta aagagttaaa ctttgacttt ggtctttaca tatataacaa
     8941 taaatttgat gaagcaaatg tagctaatac agctaagaga ttcttaacac ctatacaaat
     9001 atatgaatat gctgattttt taaatggtag acttatattt gcatttaatg acgaaaaaca
     9061 aatatatgaa aatgaatata gtttgatgaa tgtaaataat tcaaatttca cagtaagtgc
     9121 aataaagaaa gaagaaaaag gagacggagt tgtagttaga atcttcaatg gtatgaaaaa
     9181 tgaggatgct aagggaagtc ttaacatcaa taaaaatatc aatggtgctt atagtgcaat
     9241 gctcgacgaa atctatgata atactaataa gcttaaagta aatactaaaa ctgtagaagt
     9301 aaattctttg tcacattgta aagtacaaac aatagtaatt aaataatata aaatagctta
     9361 tacaagattt caaaagaaat tcctaacctt gtattaatag taaatacaag tttacactat
     9421 ttagaatatc tatgacacta aataacattt tgcaaaataa aaagatgaat cctaatatat
     9481 taggattcat cttttttaaa taaatcagca ttctgttttg aaataagatt tacaagttgt
     9541 tttgaaataa tgcttacaaa ttattttaaa acaataccta taaattttta tttaaagaat
     9601 acacataaag atttccagac tcttttattc tatagacttt ataattatta aagaagtcta
     9661 atttactcta atttttgtta aaagtaattg taactttaaa taaatcacca tccacactta
     9721 tattcatctt accaccctgt aattctataa aactttttgc tattgctaaa ccaagtccag
     9781 aaccttctgt gttacgtgac ttatctcctc ttacaaatct ctccattata tcatctacac
     9841 taaaatcaat ctcagttgca gacatatttt taagcataat atttactcta ttgtctgttt
     9901 ctaaaatatc aatatacact cttgaatttg gcattgcata tttgcttata tttattataa
     9961 gattttcaaa tactctaaat gtacgctgac tatctagtgg taatattatt ttctcactag
    10021 aaaaattatt ttttaatatt agagatgaat ctgcaagttt atcatctaat tctaaaagtg
    10081 tttgtttcat taaagataca atatccacat ttactatatt caaatgaaca tctccactat
    10141 ttgccttact tacttcgaac aagtcttcta ttaaatgctg taatctttgt gactttctat
    10201 ctaatgtatc aatatacaat ttacgatttt cgtctgttat actttcatct tttaataaat
    10261 ctatataagt gattatagat gtaagaggtg tttttaggtc atgtgataca tttgatataa
    10321 gctctgtttt catcttttga ctcttaacct cttcatcaac agctttttta aatccagttt
    10381 gaatatttcc tagttcttct tttataggat taaaaaatcc taagtccttc tccatattta
    10441 cttctaagtt tccatctgaa attttctttg taacatctaa aagttgatta tattcacata
    10501 ttgtcttttt agagtaatct gacaatatct taaatagtac tgctgaatat atcaatgcga
    10561 gtattattcc aaagaaccac atactacaca ttataactat aattaataaa tttatcccca
    10621 aaattataac tatagttttg tttggctttt cttttagatt tattgttcta atttgattaa
    10681 taactgtttt tcctgctttt tttaaccata caaaaaatat tcctgtcaca gtatttttta
    10741 taaaatattc tcttattcca acatttaaaa tatgttttaa aagtgttata aatacaaata
    10801 ttattgagaa tattacaaac caataagtta aattcattaa acttaccaat atactagccg
    10861 attcagcact tatatcattg tttatcagat actctacata atttccattc aaagtatcac
    10921 ttattatacc agtaggatat aaatctacaa tcccttgtat aatagcaaga ataataaaaa
    10981 gtatctctaa tggtattctt gataatgtat caaatcctag taattcctta gattttctgt
    11041 atgggaatac tagagctaca atcacaacta tagctaatat aatttttaaa accattctag
    11101 aaatttcata gtaagagttg ttttctacat tgtttatata cgaacttata ttatcattac
    11161 tatacatatt tccataacta tactctctac tatcgccttt acttattact ttaggtacag
    11221 catatattag tgtcatgttt tttattggag ataacttata tgaaattgta tttccaccta
    11281 aatctatatc cattaaatct tctcttgatt tagataacaa catttgatta ataacttttt
    11341 gatttactcc ataagaatcc acaacattta cttttccatc tttatcaaaa ttcattacaa
    11401 catagaatct atagttttcc tttatatctg taatctgttt ttgtctttcc tcatcattca
    11461 tttctgtatt ttttgactgg ttttcatcag aatcttcctt tgaaacatca ttctttgtcg
    11521 tatcaagtgc tcctaattct ttattatcat taaaaaattc aaatttactt tctttatcaa
    11581 ctaaataata ttttaaattt tttcttgact ctttaatgaa agactcccaa ctttcaaagt
    11641 tttcattaaa atctgcatta atactatcat ctgtataata agtttctgaa ccatcataag
    11701 tatctttttc tttacttaca ttttttatat agttcatagt ttcttcattg ataaatagct
    11761 ttgatggttg tatcatctta ccttcttgtt cttccttcat attatgatat aaaacataac
    11821 ttcctttata tatactatct acaaagcttt cctgttcaaa tggattcttt gctccacctt
    11881 tagcacctgc ttgtatagta gaatattgac taatcatacc taatgatgct gataacataa
    11941 gtataaaaac tataatagta actatatatt tatctttaaa ttttttcaat tttgtatcca
    12001 acaccccaca ccacctttaa atatttagga ttttttgggt caatctctat cttttccctt
    12061 atattcctca catgtaccat aactgtatct gtatttacag cattttcatt ccaaactctt
    12121 tcatatattt catcagcaga aaaaactctt cctgcatttc tcataagtaa acttagtatt
    12181 ttaaattcta taggagttgt ttttatatac tttccatcaa tactaacttc ttttgtgttt
    12241 tcatttaatt ctaatccacc tattgcaaac acactatcat cactttctac cttttgctct
    12301 ttatttccaa ccatgtttag atatttagtg taccttctaa gttgtgaatt aactctagcc
    12361 aatagttcta atggcttaaa aggttttgtt acatagtcgt ctgctcctat attaagcccc
    12421 attattttgt caacttcttc tgattttgca gatagcatta ttactgggaa attatagttt
    12481 tctctaagct tcattgtaag tgttatacca tccattttag gcatcattat atcaactata
    12541 gctaaatgta tttcttggtt ttctataacc ttaagacctt ctaatccatt gtatccctta
    12601 aaaacattat atccttgatt taatagatat atttctatag catctgctat ttccttttca
    12661 tcttctacca ccaaaatatt atatgaattc attctaatct cctttatatt aatttataaa
    12721 ttttctttac atatatgatt atacaatatg atagaagttt ttccatttat atatatattt
    12781 atatcttttg ttctg
//
LOCUS       DAEPXF010000105        12695 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain HB27
            SAMN13376389-rid12479423.denovo.111, whole genome shotgun sequence.
ACCESSION   DAEPXF010000105 DAEPXF010000000
VERSION     DAEPXF010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13376389
            Sequence Read Archive: SRR10518432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 12695)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12695)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001069624.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001069624.1
            Genome Coverage       :: 315x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/15/2021 01:54:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,165
            CDSs (total)                      :: 4,076
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 89
            rRNAs                             :: 10, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 10 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 19 of 69
            Pseudo Genes (incomplete)         :: 44 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 10 of 69
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12695
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13376389-rid12479423.denovo.111"
                     /strain="HB27"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="China: Hebei"
                     /collection_date="2017"
     gene            125..793
                     /locus_tag="KRL67_002554"
     CDS             125..793
                     /locus_tag="KRL67_002554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH1523422.1"
                     /translation="MSKLESTTEKVEVKWYGYVALILGALFFSGIFKDATGPLKVLDF
                     NNVLGSFGTLGTVNDGVGTLAANFRGDGGTGPRDGWLYALTLIPSVMFALGVVRVIDH
                     LDGMKAAQKLLSPLLKPLLGLPGFAGLTLIASLQSTDAAASMTKELKDDGYIDEKQKA
                     VFCAFQFSGASAITNFFASGAALFPFIGDVPIFIPLALILIMKFVGANLLRLYLNKFD
                     KGEA"
     gene            795..1280
                     /locus_tag="KRL67_002555"
     CDS             795..1280
                     /locus_tag="KRL67_002555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004455006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjiG family protein"
                     /protein_id="HBH1523423.1"
                     /translation="MSEVAKKEVKVKKGNVIDAFIGGARNGFQISTNSMAPNVIFGFA
                     IISVFNLTGLLDVIGTIFTPVMSIFGLPGVAATAIMTIFISMGGASGVIAGLFTAGQI
                     SKAHVAILLPMLLLSGAQIQFMGRLLGTAELKTKYYPHMFVIATLNGCIAMLIMNYFV
                     V"
     gene            1341..2462
                     /locus_tag="KRL67_002556"
     CDS             1341..2462
                     /locus_tag="KRL67_002556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 peptidase aminoacylase family protein"
                     /protein_id="HBH1523424.1"
                     /translation="MATNKDYIEYVHKVYNYLHTIPEKGFEEYKTSEFVLKELEKMGF
                     ENINKGVAGTGIIVTIDSKKEGPVFAIRADMDALEFIIDGEKKMIHACGHDGHTSMLL
                     TAAKLIKERNLVKRGKLKILFQPGEEKLYGALRVIDSGLLDDVEEMVGMHLRPGQDCR
                     LGKAIPAMYHCASCIMEFTIKGTTCHGSKPHLGVNAVDAVCLAVNAINAIRVNPAIPN
                     SIKVTNISVKGETYNNIPDEAFMALDIRAESNDIIEELKEKAIHAVKTAVSSINAEAV
                     LLSKDGVPAAEFDEELVNLAEETIKDVLGADASIGIFKNSGGEDFHYYTQKLKCKTTY
                     IGLGADATPGFHNPNVTFDTKALEYGVDIWCRLVEKRLG"
     gene            2744..3934
                     /locus_tag="KRL67_002557"
     CDS             2744..3934
                     /locus_tag="KRL67_002557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HBH1523425.1"
                     /translation="MSKYNFDRVIDRVGTDCVKWDFRTNCSTKAQKDGLPFWIADMDF
                     ECAEPIIEALRKKVEHKIFGYSSNDSDKYFNAVCDWYKRRFNWEINRKDIIFSPGVVP
                     AVAILVRILTNSNEGVIVQKPVYYPFEAKIKSNNRDVVNNPLIYENGTYRMDYDDLEE
                     KAKCSNNKVLILCSPHNPVGRVWREDELKKVVEICKKYDLWIIADEIHSDLIRKGFKH
                     TPLQSLCPEYKDKIVTCIAPSKTFNLAGMQLSNIIVNNDELKKKYQEEVTAVGVATSP
                     NPFAIVATIAAYNESEDWLNELNDYLDNNIQFIDEYLKENLPKVKLVYPEGTYLAWLD
                     FTAYGLNEVELEDLMLKKANVLFDEGYIFGKEGIGFERINVACPQSLLKECMDRLKTT
                     FENL"
     misc_binding    4068..4323
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            4382..5383
                     /locus_tag="KRL67_002558"
     CDS             4382..5383
                     /locus_tag="KRL67_002558"
                     /EC_number="6.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--ammonia ligase"
                     /protein_id="HBH1523426.1"
                     /translation="MSTIIPKDYKSSLNVIDTQIAIKKLKDFFEMRLSNELNLIRVSS
                     PLFVLPETGANDNLNGEKAVSFDIPFMNKNAEIVQSLAKWKRLALKKYGFEVGSGLYT
                     DMNAIRKNEELDNIHSLYVDQWDWELVIDKSSRNEKTLKDVVKRLYGVFKDTEIFVCS
                     MYEGIKEILPEEITFITSQELEDMYPELSPKEREDKIVKEKKAVFITQIGKTLISGEK
                     HDGRSPDYDDWELNGDILFYNPVLDSALELSSMGIRVDEESLDRQLKLAGCEERKEFD
                     YHKMLLNGELPYTIGGGIGQSRICMYFLQKAHIGEVQVGVWPQDMIQNCSSAGIELL"
     gene            5487..5876
                     /locus_tag="KRL67_002559"
     CDS             5487..5876
                     /locus_tag="KRL67_002559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004455003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1232 domain-containing protein"
                     /protein_id="HBH1523427.1"
                     /translation="MEFLKIIINIVLDILKKILVRFKNAKFGLVFVFDLLKLPDFMTD
                     KRINIVDKIKVISVLIFTISYFVSGVDIIPEMIAGAFGFIDDAIVLIWSIGIVNEEIN
                     KYRVITKKDKHSNIIENVEFSIKDEEE"
     gene            6037..7245
                     /locus_tag="KRL67_002560"
     CDS             6037..7245
                     /locus_tag="KRL67_002560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate/amino acid:cation symporter"
                     /protein_id="HBH1523428.1"
                     /translation="MKKKIGLVPKLIIGIIVGILIGSYAPEIIVQILVTVSTLFSAFL
                     KFVIPFIIIGFVTAGIADLATGAGKLLGITTGIAYGSTLIAGLLSFAVSTLIFPNFID
                     ASVASQIGDPEAGMLAPIFTIPLEPMVDVTAAIVFAFVMGLGISALRNHGKGETLFNF
                     FQEFQGIVTKTLSTVIIPLLPLYIAGTFANITIAGEVWTILGVFWKVYLVVIPLHFVY
                     MACQFTAAGVFSGKNPVRMLKNQVPGYLTAIGTQSSAATIPVNVGCAEKNGVSKQIRE
                     FVVPLCATIHLSGSMISITGFTTAVLMMNGMPHGIGVLFPYIAMLGIAMVAAPGAPGG
                     AIMSALPFLPMVGIPSDGGLASLMIALYLTQDSFGTACNVSGDNAIAVVVDKINDGMS
                     KKGNKEEQSA"
     gene            complement(7332..8408)
                     /locus_tag="KRL67_002561"
     CDS             complement(7332..8408)
                     /locus_tag="KRL67_002561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003415975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HBH1523429.1"
                     /translation="MSTNHGANLYSLSSKYGFSKEEFMDFSSNINPFGTSSLAKQYIV
                     NNIDMVSMYPDPDYIDLKTSISNYCKCSIDNIVLGSGATELISSFIHTINPKQALLLS
                     PAYSEYEKELSKINCSIEKYFAKEEDNFHINLENLIKTINAKDYDLVVICNPSNPTGF
                     AFTKVEVREILKNTDSFLMIDETYVEFTDTDTYSCTQLVDDYSNLFVIRGTSKFFSTP
                     GIRLGYGLISNTNVKNEINKNLDLWNINIIASKMGEIMFSDLDFISNTISLMNTERDY
                     LLKELKNIKSLDIYNTKGNFILCKIKTKELTAKSLREQLLPQKIIIRDCCSFEGLDEY
                     FFRVCILKPNENKLLISSLKAIFK"
     gene            8637..9164
                     /gene="hpt"
                     /locus_tag="KRL67_002562"
     CDS             8637..9164
                     /gene="hpt"
                     /locus_tag="KRL67_002562"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HBH1523430.1"
                     /translation="MYKVTGKMLTEEQIKEKVYELGKKIEEDFKGEDLLVVGILKGAS
                     VFVSDLIRCIDLDVNIDFMSVTSYGNSTESSGTVKILKDLDVDIEGKNVLIVEDIIDS
                     GLTLSNLVAALKTRNPKSLKLCTLLDKPQRRKANIPVDYVGFVIEDKFIVGYGIDYAE
                     KYRNLPYIGIVEDVE"
     gene            9253..10185
                     /locus_tag="KRL67_002563"
     CDS             9253..10185
                     /locus_tag="KRL67_002563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HBH1523431.1"
                     /translation="MDIQLNILLFFQNLRNPILNFIFLVFTISTEAPLLILITTIIYW
                     CINKKCGQKILFAIIGNFVVNLGIKEFVKAPRPIGIKGLESLRVSTAGGYSFPSAHTQ
                     TATTFWVSIMTIFKKRYLHLIGAVMVLGVGLSRLYLAVHWPIDVIAGWILGIFFTVIF
                     VKIFDYIDDNKNYILLVVLLIPFIIVAIFLNSPEYFEKFGIIVGFVFGYMVEDRFVKF
                     NTDNNNKKINFSNKNKGSKNIIFSRICRFLVGIVTIGMLYIGIKFFISMLIVTLNISD
                     STMSIIFMNFIKNTVVVFYGIAGAPALFKLLKLN"
     gene            10247..11473
                     /locus_tag="KRL67_002564"
     CDS             10247..11473
                     /locus_tag="KRL67_002564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HBH1523432.1"
                     /translation="MKKVVIIGGGPAGMIAASTACEKGYDVTLIEKNHKLGKKLAITG
                     KGRCNITNACEIEELIENVPTNGKFLYSAFYTFTNDDVISMFNNLGVKTKTERGKRVF
                     PESDKAFDIVNALERQLKSKKVNILLNSKVEKIISKNNKIEKVILNDKKEIKCDSVVV
                     ATGGLSYPLTGSTGDGYKFAISQGHTIIDTKPSLIGIEVQESFTKDLEKLSLRNVEIR
                     VFNSKQKKVYSDFGELEFTKFGLDGPIIKSASCRMKDTRKENYTILLDLKPALDEEKL
                     DKRVQKDFQKYTNKKFEKALDDLLPKKLIPIIINLSEINPNTVVHQISREQRKNLVHL
                     LKNLKFTVKRYRPIEEAIITSGGVKVNEINSSTMESKLVEGLFFAGEVIDIDAYTGGF
                     NLQIAFSTGYLAGFNC"
     gene            11557..11778
                     /locus_tag="KRL67_002565"
     CDS             11557..11778
                     /locus_tag="KRL67_002565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000002841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta-type small acid-soluble spore
                     protein"
                     /protein_id="HBH1523433.1"
                     /translation="MASNNNNNRTVVPGAKEALNQMKLEIANELGMSNYQQVDKGNLT
                     ARENGYVGGYMTKKLVEMAEQQMAGKSNR"
     gene            11967..12137
                     /locus_tag="KRL67_002566"
     CDS             11967..12137
                     /locus_tag="KRL67_002566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH1523434.1"
                     /translation="MSSVNFEDLKNKFINSDLDEKIRIYTTTEGLSVEQFKELLKYYP
                     IQHLSKLEKALG"
     gene            complement(12230..12691)
                     /locus_tag="KRL67_002567"
     CDS             complement(12230..12691)
                     /locus_tag="KRL67_002567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH1523435.1"
                     /translation="MQIEQLDLETRNKIYCYTKKILRKYQKGITSGKLTADKFADNIL
                     SQDFISSILNEKIVNETNFKISYRNYIETLINIQNENLSNLRKKSTKTAKCFNISQIT
                     QLKNLLSNTGYNLLIPYKYLTARDIEGIVTLINTGSIELGNERIYNYISKA"
BASE COUNT         4896 a         1327 c         2267 g         4205 t
ORIGIN      
        1 ttaatggaat aaatttacaa taaaaaaata aatacagagg ctttatcatt aaggtattgg
       61 cataagtttt gcttattaaa caagtatact aataaaattc atttaaacag gagggagaat
      121 ttatatgagt aaattagaat caacaacaga aaaagttgaa gtaaaatggt atgggtatgt
      181 agcattaata ttgggagcat tatttttttc aggaatattt aaagatgcga caggtccatt
      241 aaaagtattg gatttcaaca atgtattagg ttcttttgga acattgggaa cagttaatga
      301 tggtgtaggt acattagctg caaactttag aggtgatgga ggtactggtc ctagagatgg
      361 atggctatat gcattgacat taataccatc agttatgttt gcacttggag tagttagagt
      421 tatagaccac ttagatggaa tgaaggcagc tcaaaagctt cttagtccac tattaaagcc
      481 attattaggg ctacctggat ttgcagggct aactttgata gctagtttac agtctacaga
      541 tgcagctgca tctatgacca aagaacttaa agatgatgga tatatagatg aaaaacaaaa
      601 ggctgtattt tgtgcatttc aattttcagg tgcgtctgca attacaaatt tctttgcttc
      661 tggagcagca ctatttccat ttataggaga tgtaccaata tttataccac ttgctttaat
      721 attaattatg aagtttgttg gggcgaactt acttagatta tatttaaata aatttgacaa
      781 gggggaagct taacatgagt gaggtagcaa aaaaagaagt aaaagttaaa aaaggaaatg
      841 tgatagatgc gtttattgga ggagctagaa atggattcca aataagtaca aattcaatgg
      901 ctccaaatgt catcttcggt tttgctataa tttctgtgtt caatttaaca ggtcttttgg
      961 atgtaatagg gacaatattt acacctgtaa tgagtatctt tggacttcca ggtgttgcag
     1021 caacagctat aatgacaata tttatatcaa tgggaggagc atctggagta atcgcaggtt
     1081 tatttacagc aggtcaaata agtaaagcac atgttgcaat tctattacca atgttattgc
     1141 tttcaggagc gcagatacaa tttatgggga gacttttagg aacagctgag ttaaaaacta
     1201 aatattatcc acatatgttt gtgatagcaa ctttaaatgg ttgtattgca atgcttataa
     1261 tgaattattt cgtagtatag gtaattataa taaaaatttg atataaatgg aaagtttaaa
     1321 ttttaaggag gagctatatt atggcaacta acaaagatta tattgagtat gtacataaag
     1381 tgtataatta tctacacact ataccagaaa aaggatttga agaatataag acatcagagt
     1441 ttgttttaaa agagcttgaa aaaatggggt ttgaaaatat taataaagga gttgcaggaa
     1501 ctggtattat agtaactata gattctaaaa aagaaggacc agtatttgct ataagagcag
     1561 atatggatgc attggaattt atcatagatg gagaaaagaa aatgatacat gcttgtggtc
     1621 atgatggtca tacttctatg cttttaacag ctgcaaagtt aataaaagag agaaatttag
     1681 taaaaagagg taagttaaaa atactttttc aaccaggtga agaaaagtta tatggagcat
     1741 taagagtaat tgattctggt ttgttagatg atgtagaaga aatggtaggg atgcatttaa
     1801 gacctggaca agattgtagg ttgggaaagg cgatacctgc aatgtaccat tgtgctagtt
     1861 gtataatgga atttacgatt aaaggtacca cttgtcatgg ttctaaacca catttaggag
     1921 taaatgcagt agatgctgtt tgtttagcag tgaatgcaat aaatgcaata agagtaaatc
     1981 cagctatacc aaattctata aaagtaacaa atatatcagt taagggagaa acttataata
     2041 atataccaga tgaagcattt atggctttag atataagggc agaaagtaat gatataatag
     2101 aagaattaaa agaaaaagca atacatgctg taaaaactgc tgtaagttct ataaatgcag
     2161 aagctgtact gctttccaaa gatggagtac cagcagcaga atttgatgaa gagcttgtaa
     2221 acttggctga agaaactata aaagatgtat tgggagctga tgcatctata ggtatattta
     2281 aaaattctgg tggagaagat ttccattatt atacacaaaa actaaaatgt aaaactacat
     2341 atattggatt aggagctgat gctacaccag gatttcataa tccaaatgta acatttgata
     2401 cgaaagcttt agaatatggt gtagatatat ggtgtagatt ggttgaaaag agattgggat
     2461 aatatccaaa ctaataattc tacatttaaa attataatta tagaagttat agaaaaagat
     2521 tgcttatgtt aattagaata taattaatat gagcaatctt attttgatat aatgaaactg
     2581 gttttggaat attgaaaaaa atatgatgga aaagtaatta tattttaaat ctattggaca
     2641 aaaaagtgat attattataa tattaagaat tgtaaaataa tattaaattg catctagtaa
     2701 tggacttaac aattataaaa attttattct taggagggaa aaaatgagta agtataattt
     2761 tgacagagta atagatagag taggaacaga ttgtgtaaaa tgggatttta gaactaattg
     2821 ttcaacaaaa gcacaaaaag atggtttgcc tttttggatt gctgatatgg attttgaatg
     2881 tgcagaacca ataatagaag cattacgtaa aaaagttgaa cataaaatat ttggatatag
     2941 ttcaaatgat agtgataaat actttaatgc agtttgtgat tggtataaaa ggagatttaa
     3001 ctgggaaata aatagaaaag atattatttt tagtcctggt gttgtacctg ctgttgctat
     3061 tttagtaaga atattaacta attctaatga aggtgtaata gtccaaaaac cagtgtatta
     3121 cccatttgaa gctaagataa agagcaataa tagggacgtt gtaaacaatc ctctaatata
     3181 tgagaatggt acttatagga tggattatga tgatttagaa gaaaaagcta agtgtagcaa
     3241 caataaagta ctgatacttt gtagtcctca caatcctgtt ggaagagttt ggagagaaga
     3301 tgaattaaaa aaggttgtag aaatatgtaa aaaatatgac ctgtggataa tagctgatga
     3361 gatacattct gatttaatta gaaaaggatt taaacatact cctttacagt cattatgccc
     3421 agagtacaag gataaaatag ttacatgtat agctccaagt aaaacattta atctggcagg
     3481 aatgcagttg tctaatataa tagtgaataa tgatgaacta aaaaagaaat atcaagaaga
     3541 ggttacagct gttggagttg caacttcacc aaatcccttt gctattgtag ctacaatagc
     3601 agcatataat gaaagtgaag actggcttaa tgaattaaat gattacttag ataataatat
     3661 acaatttatt gatgaatact taaaagaaaa cctaccaaaa gtaaaacttg tttatccaga
     3721 aggaacatat ctagcatggt tagattttac tgcttatgga ttaaatgaag tagaacttga
     3781 ggatttaatg cttaaaaaag ctaatgtatt atttgatgaa ggttatatat ttggaaaaga
     3841 aggaatagga tttgaaagaa taaatgtagc ttgtccacag tctttattaa aagaatgtat
     3901 ggacagatta aagactacat ttgaaaattt ataaagtata ggtaaaaatt tataaagtgt
     3961 ttaaaaataa aaaaataata aacttgacaa attttaacta atcacttatc atactattat
     4021 gaataaatag aaaacataag agagctaaat tttattgaat taaactctta aagactttga
     4081 aaggaagagt agttatagaa tgtaattaaa gcgagttggt gatggtgtga gaccaatatg
     4141 aagtctatag tgaagaacat cctggagttg ctaaccgaaa ttttaaaaga gtaggcttag
     4201 tcggaaccaa accgttatca tattggggtg tatcgagtaa tatctgtaca ttgtgagctg
     4261 ggtttgaatt tatttcaaac taatcagggt ggtaccgcgg aaaagcaacc tttcgtccct
     4321 tttatatagg gatgaagggt ttttttaatg gaaaaatata taaatatagg gagtgaatga
     4381 tatgagcaca ataataccaa aggattataa aagtagtttg aacgtaattg atacacaaat
     4441 agctataaaa aaattaaaag atttttttga aatgagacta agtaatgaat taaatcttat
     4501 aagagtttct tctccgttat ttgtattacc agaaacaggt gcaaatgata atttaaatgg
     4561 agaaaaggct gtaagctttg atataccatt tatgaataaa aatgcagaaa ttgttcaatc
     4621 acttgcaaaa tggaaaagat tagcccttaa aaaatatggg tttgaagttg gtagtggttt
     4681 atatacagat atgaatgcaa taagaaaaaa tgaagaatta gacaacatac attctcttta
     4741 tgtagaccaa tgggattggg aattggttat agataaaagt agtagaaatg aaaagactct
     4801 aaaagatgtt gtaaaaagat tgtatggagt atttaaagat actgaaattt ttgtatgtag
     4861 tatgtatgaa ggtataaaag aaatattacc agaagaaata acttttataa catcacaaga
     4921 gttagaagat atgtatccag aattatcacc aaaggaaagg gaagataaaa ttgtaaaaga
     4981 gaagaaagca gtatttataa ctcaaatagg aaaaacatta atatcaggag aaaaacatga
     5041 tggaagaagc cctgattatg atgattggga attgaatggg gatatattat tttacaatcc
     5101 tgttttagat tcagcattag agttatcttc aatgggaatt agagttgatg aagaatctct
     5161 tgatagacaa ttgaaacttg ctggatgtga agagagaaaa gagtttgact atcataagat
     5221 gcttttaaat ggcgaacttc catatacaat tggtggagga attggtcagt caagaatatg
     5281 tatgtatttc ttacaaaaag ctcatatagg agaagtacaa gttggagtgt ggccacagga
     5341 tatgattcaa aattgtagta gtgcaggaat tgaattatta taatttaaat ataaactaat
     5401 tgataaaatt tatatctaat atgttcatca aattaattat atcggttata atgtagaata
     5461 acctataatt ataagaggtg ataaatatgg agtttctaaa aataatcatt aatatagttt
     5521 tagatatttt aaaaaaaatt ttggtgagat ttaaaaatgc taaatttggt ttggtttttg
     5581 tatttgattt acttaaacta cctgatttta tgacagataa aagaatcaat atagttgata
     5641 aaataaaggt aatttctgta ttaattttta ctatatctta ctttgtatct ggggttgata
     5701 taattccaga gatgattgca ggggcatttg gttttataga tgatgctata gttttaattt
     5761 ggagtatagg tatagtgaat gaagaaatta ataagtatag agttatcaca aaaaaagata
     5821 aacattcaaa tataattgaa aatgtagaat tttctataaa agatgaagaa gaataacaat
     5881 ttatatatgt tattcttttt tttattaaca aaaaatggtt aaattgttga cggagaggta
     5941 aaactctatt atcataataa gtggatatcc aatatttaac taagtattca atgtgcaaga
     6001 atgcttaatt taaaaaagtt atggagggta aatattatga agaaaaaaat aggtttagtg
     6061 cccaaactaa tcatcggtat catagtcggt attttaatcg gaagttatgc accagaaata
     6121 atagtacaaa tattagtaac agttagtact ttattctcag cattcctaaa atttgttata
     6181 ccatttataa ttataggttt tgtaacagca ggtatagctg atttagcaac aggtgcagga
     6241 aagttattgg ggataactac gggaatagca tatggttcta ctttaatagc aggattatta
     6301 tcatttgcag tatctacatt aatcttccca aattttattg atgcaagtgt tgcaagtcaa
     6361 attggagacc cagaggcagg aatgttagct ccaatattca caattccatt agagcctatg
     6421 gtagatgtaa cagctgcaat cgtatttgca tttgtaatgg gacttggaat atcagcttta
     6481 agaaatcatg gaaaaggaga gactttattt aatttcttcc aagaatttca aggaatcgta
     6541 acgaaaacgt tgtcaacagt gattatacct ttattacctt tatacatagc aggaactttt
     6601 gcaaacataa ctatagcagg ggaagtatgg acaatattag gggtattctg gaaagtatac
     6661 ttagtagtaa tacctttaca ttttgtatat atggcatgtc aattcacagc agcaggtgta
     6721 ttttcaggaa aaaatccagt aagaatgtta aaaaatcaag tacctggata tttaacagct
     6781 ataggaactc aatcttcagc agcaactata ccagttaatg ttggttgtgc agaaaaaaat
     6841 ggagtaagta agcaaataag agaatttgtt gtacctcttt gtgcaacaat acatttatca
     6901 ggtagtatga tatcaataac aggatttaca actgcagttt taatgatgaa tggtatgcca
     6961 catggaatag gtgtattatt cccttatatt gcaatgttag gtatagcaat ggttgcagca
     7021 ccaggagcac caggtggggc tataatgagt gcacttcctt tcctaccaat ggttggaata
     7081 ccatcagatg gtgggcttgc aagtttaatg atagctttat acttaactca agacagtttt
     7141 ggtacagcat gtaatgtatc aggagataat gcaatagcag tagtagtaga taaaataaat
     7201 gatggaatga gtaaaaaagg aaacaaagaa gagcaaagtg cttagttttt taataatcaa
     7261 aaatgagtta tctcaaatag agatagctca ttttttatgg tataaaaaaa ttgtaaaata
     7321 aagtgatttt tttatttaaa aattgcttta agagatgata ttaataattt attttcatta
     7381 ggttttaata tacatactct aaagaaatac tcatctagac cttcaaatga acaacaatct
     7441 cttattatta ttttttgtgg aagcagttgt tctcgaagag atttagcagt caattccttt
     7501 gtcttgattt tacacaatat gaaattacct ttagtattgt atatatctaa gctttttata
     7561 ttcttaagct ctttaagcag gtaatctctt tcagtgttca ttaaggaaat agtatttgat
     7621 ataaaatcta aatcactaaa catgatttcc cccattttag aagctattat gtttatattc
     7681 cataaatcta gatttttatt tatttcattt ttgacattag tgttagatat aaggccatat
     7741 ccaagcctta ttccaggcgt tgaaaaaaat tttgatgtac ctcttataac gaataaatta
     7801 gaataatcat caactaattg agtacaagag tatgtatctg tatcagtaaa ctccacataa
     7861 gtctcatcaa tcattaaaaa tgagtctgta ttttttaaaa tttctctcac ttctacttta
     7921 gtaaatgcaa atccagttgg attactagga ttacagatta ctaccaaatc ataatcttta
     7981 gcattgatag tttttataag attttctaaa tttatatgaa aattatcctc ttctttggca
     8041 aagtattttt ctatagaaca attaatttta gataactctt tttcatactc tgaataggct
     8101 ggagagagta aaagagcctg tttaggatta attgtatgta taaatgatga aattagttct
     8161 gtagctccac tgccaagtac tatattatct atagaacatt tgcagtaatt ggatatagaa
     8221 gtttttaagt ctatgtaatc agggtcagga tacatagata ccatatctat attgtttaca
     8281 atgtattgtt tggctaaact tgatgtacca aaaggattta tatttgagct aaaatccata
     8341 aattcttcct ttgaaaagcc atacttacta gataaactat ataaattagc tccatgattt
     8401 gtgctcataa aagtacctcc aaaaataaaa ttatataatc gataaaaata actgattaat
     8461 atatgtacat atagattata atacaaagga atcgttattc caaattttta atggaaaaag
     8521 caaaaaagta tggataagct gttaatttag gtttaaaaat gatgaaaaag attttaattg
     8581 atattttggt atgctataat atataagtta atagaaggaa agcgggagga tacaaaatgt
     8641 acaaagttac aggaaaaatg ttgacagaag aacaaataaa agaaaaagtt tatgaattag
     8701 gaaaaaagat agaagaagac tttaaaggcg aagatttact agtggtaggt atacttaaag
     8761 gagctagtgt atttgtttca gatttaataa gatgtataga tttggatgta aatatagatt
     8821 ttatgagtgt tacaagttat ggaaactcaa cagagtcttc tggaacagtt aaaatattaa
     8881 aagatttaga tgtagatatt gaaggaaaga atgtgttgat tgtagaggat ataatagatt
     8941 ctggtttaac attaagtaat ctagttgcag ctttaaaaac tagaaatcca aaatctttaa
     9001 aactatgtac tctacttgac aaacctcaga gaagaaaagc taatatacct gtagactatg
     9061 ttggatttgt tatagaagac aaatttattg ttggttatgg aatagactat gctgaaaagt
     9121 atagaaattt accttatata ggtatagttg aggatgtaga atagatttaa atgtgaatca
     9181 aaagcactaa tttctaaggg agttagtgct ttttagttaa ttatataaaa ttttagtaaa
     9241 ggagacatct atatggatat tcagttaaat atattattat ttttccaaaa tttaagaaat
     9301 ccaattttga actttatatt tcttgtcttt acaataagca cagaagcacc gttattaata
     9361 ttaattacaa caattatata ctggtgtata aataaaaaat gtggacagaa aatactgttt
     9421 gctataattg gaaattttgt tgtaaattta ggcatcaaag agtttgtaaa agctcccaga
     9481 ccaataggaa taaaaggtct tgaatcattg agagtttcta ctgctggtgg ttattctttt
     9541 ccaagtgctc atactcaaac tgcaacaaca ttttgggtta gtattatgac tatatttaaa
     9601 aagagatatc ttcatttaat aggtgcagtt atggttttag gagtaggatt gtctaggctg
     9661 tatttagctg ttcattggcc aatagatgtg atagcgggtt ggatattagg aatatttttt
     9721 acagttatat ttgtaaaaat atttgactat attgatgata ataaaaatta tattttactt
     9781 gtagttttac ttataccatt tataatagta gctatatttc taaatagccc tgaatatttt
     9841 gaaaaatttg gtataatagt aggatttgta tttggatata tggttgagga tagatttgtt
     9901 aaatttaata cagataataa taacaaaaaa ataaatttta gtaataaaaa taaggggagt
     9961 aaaaatataa ttttttcaag aatatgtagg tttttggttg ggatagtaac aataggaatg
    10021 ttatatattg gcattaagtt ttttatttca atgttaatag tcactttaaa tataagtgac
    10081 tctactatga gtataatttt tatgaatttt attaagaata cagtagttgt tttttatgga
    10141 atagcaggag ctccagcatt atttaaactt ttaaaattaa attgatttta aggtatagag
    10201 tcaatagttt aaattaaaaa aattaagggt agaaaagagt gattatttga agaaggtagt
    10261 aataataggt ggtggaccag cagggatgat agctgcatca actgcatgtg agaaaggcta
    10321 tgatgtaaca ttaattgaga aaaatcataa attaggaaaa aaacttgcaa taacaggaaa
    10381 aggtagatgt aatattacta acgcttgtga aatagaggaa ttaatagaaa atgtacctac
    10441 aaatggaaaa tttctttata gtgcttttta tacttttaca aatgatgatg taataagtat
    10501 gtttaataat cttggagtaa agactaaaac agaaagagga aaaagggttt ttccagagag
    10561 tgataaagca tttgatattg taaatgctct tgaaagacaa cttaaaagta agaaagtaaa
    10621 tatattatta aattcgaaag tagaaaaaat aatatctaaa aataataaaa ttgagaaagt
    10681 aattttaaac gataaaaaag aaattaagtg tgattcagtt gtagtagcaa ctggtggatt
    10741 aagttatcca cttactggtt ctactggtga tggatataaa tttgcaattt cacaggggca
    10801 cacaattata gatacaaaac catctttaat tggtatagaa gtacaagaaa gttttacaaa
    10861 agatttagaa aaattatcat tgagaaatgt tgaaataagg gtgtttaata gcaaacaaaa
    10921 gaaagtttat agtgattttg gagaacttga atttactaaa tttggtttag atggacctat
    10981 aatcaagtct gcaagttgta gaatgaaaga tacaaggaaa gaaaattaca ctatattatt
    11041 agatttaaaa cctgcattag atgaggaaaa attggataaa agagttcaaa aagattttca
    11101 gaagtataca aataaaaaat ttgaaaaagc attagatgat ttacttccaa aaaaactaat
    11161 acctataata ataaacttaa gtgaaattaa tccaaatacg gttgttcacc aaatttctag
    11221 agagcagaga aaaaacttag tccatttatt aaaaaattta aagtttacag taaagagata
    11281 cagacctatt gaagaagcta taataacttc tggtggtgta aaagtaaacg aaataaattc
    11341 aagcacaatg gaatcaaaat tagtagaagg tttatttttt gctggagaag taatagatat
    11401 agatgcgtat acaggtggat tcaatttgca aatagctttt tctactggat atttggcagg
    11461 ttttaattgc taaaaattac ctgaattaaa aatgtttaaa atcacataat attatcagca
    11521 acacaaaata aattaaacag aaggaaggta atcaacatgg caagtaacaa taacaacaac
    11581 agaacagtag taccaggagc aaaagaggct ttaaaccaaa tgaaattaga aatagctaat
    11641 gaacttggta tgagtaatta tcaacaagtt gataaaggaa accttactgc aagagaaaat
    11701 ggttatgtag gtggatacat gactaaaaaa ttagttgaaa tggctgaaca acaaatggct
    11761 ggaaaaagca acagatagtt ataaataatt atttataact attggctatt agtttagcta
    11821 gagctatctc aaatatagaa agtaatttct aaattgagat agcttttttt tattaaatta
    11881 ttaaatagat atatttttat gtaataaatt ttatgtattt ggataaaata atacaaatga
    11941 atatgttaca taaggagtga tgtaaaatga gtagtgtaaa ttttgaggat ttaaaaaaca
    12001 aatttattaa tagtgactta gatgaaaaaa taagaatata tacaacaaca gaaggtttat
    12061 cagtagaaca atttaaagag ctactaaaat attatccaat acaacattta tctaaattag
    12121 aaaaagcact tggatagcag tatttagtcc aaaattaaaa aggcaattta tttaatatta
    12181 aaaatatttt ttattaaata aattgccttt ttataatagg tttaaattat tatgcttttg
    12241 atatataatt gtagattctc tcatttccaa gttcaataga cccagtatta ataagagtaa
    12301 cgattccttc tatatctcta gcagttaagt atttatatgg aattaataaa ttataaccag
    12361 tattacttag taagtttttt aattgggtta tttgagagat attgaaacat tttgcagttt
    12421 tagtagattt ttttcttaag ttagataaat tttcattttg aatatttatt aaagtttcta
    12481 tatagtttct ataagatatc ttaaagtttg tctcattgac tattttttca ttaagtatac
    12541 tactgataaa atcttgagac aatatattat ctgcaaattt atctgctgtt aattttccag
    12601 atgttatgcc tttttgatat tttctaagaa tttttttagt atagcagtat attttatttc
    12661 tcgtttctaa atcgagttgt tctatttgca caaaa
//
LOCUS       DAEPXF010000106        12682 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain HB27
            SAMN13376389-rid12479423.denovo.112, whole genome shotgun sequence.
ACCESSION   DAEPXF010000106 DAEPXF010000000
VERSION     DAEPXF010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13376389
            Sequence Read Archive: SRR10518432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 12682)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12682)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001069624.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001069624.1
            Genome Coverage       :: 315x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/15/2021 01:54:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,165
            CDSs (total)                      :: 4,076
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 89
            rRNAs                             :: 10, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 10 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 19 of 69
            Pseudo Genes (incomplete)         :: 44 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 10 of 69
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12682
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13376389-rid12479423.denovo.112"
                     /strain="HB27"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="China: Hebei"
                     /collection_date="2017"
     gene            95..1585
                     /locus_tag="KRL67_002568"
     CDS             95..1585
                     /locus_tag="KRL67_002568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HBH1523436.1"
                     /translation="MFQFETQIHKLRHDVLTAIARLGKEDNLTREAMDNIQYEIIKGE
                     KPTYRCCVYKERAIISERVNVSMGLQPGQDKIDLDIMDIEEDEQIIYILEAACDSCPI
                     NEFTVTDICRGCLAHRCKEACKFGAITHVGGMAYINHELCKACGMCKKACQYDAISEV
                     VRPCKSVCPTNALGFDRENMKAMIHEDKCLNCGACMSACPFGAISDKSLIAPVARKLV
                     QKEKMYAVVAPAITGQVEANVTYGQIKNAIKSLGFVDVLEAACGADAVTVHESLEFAE
                     RMEKGEAFMTNSCCPGFVGYIEKTYPNHVGKISSTVSPMIASGRFIKSHEPDAKVVFI
                     GPCTAKKSEAAKKELKGAVDYVLTFEEIMALFEAFDIDLAKCSNEDIDDASIFGRGFG
                     AAGGLTKAIENYLAEKGIGVNFEPVKISGSREIKKTMTMANLGKLEGNFIEGMMCEGG
                     CINGSGKFLSGAKVKATFDRKNSQSTKKSVLANAKIKDFSNINLEK"
     gene            1865..3361
                     /locus_tag="KRL67_002569"
     CDS             1865..3361
                     /locus_tag="KRL67_002569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HBH1523437.1"
                     /translation="MFEFETQLKRLKHEVLTAVARLSIEENLTKENIEKIPYEIIKGE
                     KARYRCCVYKEREVVLERAQLALSLTPNSKYGDINPDSVSLDTDEQIIYIVEAACDRC
                     PINEFTVTELCRGCLAHRCKEACKFGAISYINGRAYINHDLCKACGMCKSSCQYDAIS
                     EVVRPCKSVCPTGALDFNRNTMKAMIHEDNCINCGACMSACPFGAISDKSLIAPVAKK
                     LAKKENMYAIVAPAITGQIDSNITYGQLKNAIKSLGFVDMIEASCGADAVTVHESNEL
                     IERIKSGDNYMTNSCCPGFFKYIEKMFPDEKDRISSTVSPMVATGRLVKKIDSDAKVV
                     FIGPCTAKKSEATEISLKDSVDYVLTFEELMALFEAFNIDLSKCSNEDINDGSIFGRG
                     FGASGGLAKSVQNYIAEKGLSFDFEPIKVSGPRQIKKTMTMAKLGRLEGNFIEGMMCE
                     EGCINGAGKFLSGVKSKNTFDRKNGQSTKKSVLSNDKIKEFEDVNLER"
     gene            3495..4409
                     /locus_tag="KRL67_002570"
     CDS             3495..4409
                     /locus_tag="KRL67_002570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HBH1523438.1"
                     /translation="MKKIILKDILFLVTTLSIIVILLLVTNITANSKIKSTSETYLKS
                     DKNSVSQNGIQSNTKNTAFKTTILSTGKSDCILVEIGNKVIMIDTGEDKNGKQIVARL
                     KEKGINTLDYLILTHLDKDHIGGVDSVLSSVKVKNIIQANYKKDSKQYDEYIDSLKKA
                     DIEPVLLKDNMNIVINSAEINIQPASKSKYESSNDYSIITNISYGAHKFLFAGDAEEE
                     RLSEFINGNTLKYDFVKIPHHGRYDKLTEAFLESISPQCAVITCSEKKEPEEGVLKIL
                     ERLNIKTFLTSNGEVVINSDGKTLSVNQ"
     gene            4527..4718
                     /locus_tag="KRL67_002571"
     CDS             4527..4718
                     /locus_tag="KRL67_002571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH1523439.1"
                     /translation="MLDRFFGEGGFGGCAWWIIILFFLFLAFQDCWAEMDLCAWIPFL
                     ILLLILCECGDVFDGGCGC"
     gene            4941..5291
                     /locus_tag="KRL67_002572"
     CDS             4941..5291
                     /locus_tag="KRL67_002572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH1523440.1"
                     /translation="MAIVDKIFNEIKLMVARLLLGKKYSEYEEKDLVYNTEEEVILIT
                     LKRLVFQGNVNEAEEILFDKAKSVNSENMQYIAIEFYTMLMEKTDEELEAMNFSKQEV
                     YQGIEDIRKVLNLE"
     gene            5552..7057
                     /locus_tag="KRL67_002573"
     CDS             5552..7057
                     /locus_tag="KRL67_002573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003374392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1846 domain-containing protein"
                     /protein_id="HBH1523441.1"
                     /translation="MKVGFDHKKYLEEQSKYILERVNNFDKLYLEFGGKLMGDLHAKR
                     VLPGFDENAKIKVLQHMKEKVEVVICVYAGDIERNKIRGDFGITYDMEVLRLIDDLRG
                     YELDVNSVVITRFEGQPATTVFINKLERRGIKVYKHYPTKGYPSDVETIVSDEGYGAN
                     EYIETTKPIVVVTAPGPNSGKLGTCLSQLYHENKRGNVVGYSKFETFPVWNVPVKHPL
                     NIAYEAATVDLKDVNMIDSFHLETYGEMSVNYNRDLELFPVLKKIIEKITGKESIFQS
                     PTDMGVNRVGFGIIDDEVIRKASIEEIIRRYFKTACEYKKGQVDKGAYDRMKMIMEEL
                     NLKPEDRNVVLPARNYSNKLKESADKNDTCPVVALELEDGTILTGKGSDLMNGTAAVV
                     LNAIKHLANISDDMHLISPVILEPIINLKTKTLASRNVSLSCQEVLTALSICAVTNPT
                     AQFAMAKLALLKGCQAHSTTILNRDDEQTFRKLGIDVTCDPEYPSQNLYYS"
     gene            7448..8545
                     /gene="dinB"
                     /locus_tag="KRL67_002574"
     CDS             7448..8545
                     /gene="dinB"
                     /locus_tag="KRL67_002574"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HBH1523442.1"
                     /translation="MKLHRKIIHIDMDAFYASIEQRDNKKLKGRPVIVGGNPQSRGVV
                     ATCSYEARKFGIHSAMPSAVAYNRCPYAVFVRPRFDVYKSVSEQIRDIFYRYTDLVEP
                     LSLDEAYLDVTKNKKSIDSSIEIAKQIKKDIFREVGLTSSAGVSYNKFLAKIASDLRK
                     PNGLTVITEENAQDFLDKLPVNKFFGVGKVTSNTLKNLGIKTGYDLRCLNLFELENIF
                     KKRGYELYKFARGIDDRPVEPNRVRKSVGAETTLSHNLDIDEEETRNILDELCEEVCH
                     RLKSSEKFGKTLTLKIKYEDFTKITRSISLEHYIDEYNDIRSGVDNLLRNVEVNGKQI
                     RLLGVTISNLSDKKETYKDITLFEYMDSIQM"
     gene            8808..9944
                     /locus_tag="KRL67_002575"
     CDS             8808..9944
                     /locus_tag="KRL67_002575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBH1523443.1"
                     /translation="MIEIRNVTKKIGNNVILDDISLVVETGTLVVLIGSSGCGKTTTL
                     KLINKLIKPTSGEIYINGKPISQENEIELRRKIGYVIQNTGLFPHLTIKENIELIPRL
                     KKEKSVEEIEKRTLQLLEMVGLDSDEFLNKYPSELSGGQQQRIGVARAIATDAEIILM
                     DEPFSALDPITRTSLQEQLFSLQDELKKTIIFVTHDMDEALKIADKICIMKDGRIAQY
                     DTPENILRKPANDFVKDFIGEDRVWDNPEYIKARDIMIKNPIAVNSTRTVTQGIEIMR
                     TSKVDSLLIIDRAKTLKGIVTVKDMKDIDDKSILLADIMSSEPLHVNEGDNLVEILNV
                     MNRNSVGYIPVISDENKLVGLITRSSLLSVLSEQFLEMEVSVLG"
     gene            9937..11484
                     /locus_tag="KRL67_002576"
     CDS             9937..11484
                     /locus_tag="KRL67_002576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HBH1523444.1"
                     /translation="MANFFNFILLQKDKIIELLIQHMSLTVTSILIAIIVGVPLGIII
                     SRISSLRKFVLGFVNLVQAVPSMALLGLLVPILGIGSKPAIFMVVVYSLLPIVKNTYI
                     GITSIDPVVLESAKGIGLTRNQTLFKIQFPLALPIIMGGVRISAVTAVGLMTLAAFIG
                     AGGLGYLVFSGVQTVNNNMILAGAIPSCIIALLVDYLFSKIEVAVTPKGLNPKAPKKN
                     YIALKVISVVLVVSMLFVVFSSSFSSKKDTITIGSKDYTEQLILGNVYAELVEKNTNL
                     KVKKNLNLGGSSVAFNAIKSGELDMYVDYTGTLLVNVMKHAPIKDADEAYNVVKDTME
                     KENQLTLLDPLGFNNTYTLAMMPETAEKYGINTISDLTKYGKEFTFSPTLEFENREDG
                     LVGLSRDYGLKFKDVKAMNGSLRYTALDNNESQVIDAFLTDGLIKKFNLKILEDDKNF
                     FVPYYAAPLVREDTLKKYPELEKVMNMLSGKVNEETMRELNYQVDELGKSPEEVAHSF
                     LVKEGLV"
     gene            11818..>12682
                     /locus_tag="KRL67_002577"
     CDS             11818..>12682
                     /locus_tag="KRL67_002577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HBH1523445.1"
                     /translation="MDNYKKVKQVLWIILFANFAVALLKIIIGNQIKSYSMTADGFHS
                     LSDGASNIVGLIGIFFASKPKDKNHPYGHKKFEIITSLFISGMLFVIAIKIILSAVLR
                     ITNPVVPAITIESLIALIITLFINIFVCMYEYRVGTKLNSYVLISDSLHTRSDIFVSL
                     GVLVTLVGVKLGFPVIIESIVPIIISAFIIYSAYGIFRPSIGILVDRVAVDEDYIKEI
                     VFEFNEVRDVHNIRSRGSKSSIYIDMHVMVDPFISVEQSHDLTHKIEKQIQEEINENA
                     QVIVHIEPFYSF"
BASE COUNT         4902 a         1341 c         2327 g         4112 t
ORIGIN      
        1 atttaaacat ataatatttt atgtatatat taataaaagg acatattgtg ttataatggt
       61 atcgtataaa atcatttacg ggggtggtat taaaatgttt caatttgaaa cacaaataca
      121 caaattaaga catgatgttt taactgcaat agctagactt ggaaaagaag ataaccttac
      181 cagagaagca atggataata ttcaatatga aataataaaa ggtgagaaac ctacatacag
      241 atgctgtgtt tataaagaaa gagctataat atctgagaga gtaaatgtaa gtatgggatt
      301 acaaccaggt caagataaga tagatttaga tataatggat atagaagaag atgaacaaat
      361 tatatatatt ttggaagctg catgtgacag ttgtccaata aatgaattta cagtaacaga
      421 tatttgtaga ggatgtctag cacatagatg taaagaagct tgtaaatttg gagctataac
      481 acatgttgga ggaatggcct atataaatca tgaattatgt aaggcttgtg gaatgtgtaa
      541 aaaagcttgt caatatgatg ccatctcaga ggttgttaga ccatgtaaga gtgtttgtcc
      601 aactaatgcc ttaggatttg atagagaaaa tatgaaagct atgatacatg aggataaatg
      661 tttaaactgt ggagcatgta tgtcagcatg tccatttgga gcaatatctg ataagagttt
      721 gatagctcct gtagctagaa aattagttca aaaagagaaa atgtatgcag tagtagctcc
      781 agctataaca ggtcaagttg aagcaaatgt tacatatggt caaataaaaa atgctataaa
      841 atcactagga tttgttgatg tattagaagc agcttgtgga gcagatgcag ttacagttca
      901 tgaaagttta gaatttgctg aaagaatgga aaagggagaa gcttttatga caaattcttg
      961 ttgtccaggg tttgttggtt atatagaaaa aacataccca aatcatgtag gaaaaatatc
     1021 ttctacagtg tctcctatga tagctagtgg tagatttata aaaagtcatg aaccagatgc
     1081 taaagttgta ttcataggac catgtacagc taaaaagagt gaggcagcta agaaagaatt
     1141 aaaaggagct gttgattatg tacttacttt tgaagaaata atggcattat ttgaggcatt
     1201 tgatatagac ttggctaagt gttcaaatga agatatagat gatgcatcaa tttttggaag
     1261 aggttttgga gctgccggtg gtcttacaaa agctatagaa aattatcttg ctgaaaaagg
     1321 tattggtgtt aattttgaac ctgtaaagat ttctggttca agagaaatca agaaaactat
     1381 gactatggct aatttaggaa agctagaagg taattttata gaaggaatga tgtgtgaagg
     1441 tggttgtata aatggttctg gtaaattctt atcaggtgca aaggttaaag ccacttttga
     1501 tagaaaaaat tctcaatcaa ctaagaagag tgtcttagca aatgcaaaga taaaagattt
     1561 tagtaatata aatttagaaa aataaagata atacaaagca acaaaaatac atattgttaa
     1621 tagagttgtt ctaagttgaa gtttaacttt tggacaactc tatttttatt tatatattta
     1681 attattgaac aactaaaata aaaattatat acacttaaaa taaaaattac atatataata
     1741 ttttgtatat aacttaacaa agtatatgcc tatgttataa taatttatag aaataaataa
     1801 aaaatacaat atacataata gtagaaatat agcaaaaagg tgtgatttat agggaggatt
     1861 agttatgttt gaatttgaaa cgcaactaaa aaggttaaaa catgaagttt taacagcagt
     1921 agcaagacta tctattgaag aaaatttaac aaaagaaaat atagaaaaaa ttccatatga
     1981 aataattaaa ggagaaaagg cgagatatag atgctgtgtt tataaagaga gagaagtagt
     2041 tcttgaaagg gcgcaacttg cattaagttt aactccaaat agtaaatatg gagatataaa
     2101 tccagattca gttagtttgg acacagatga gcaaattata tatatagtgg aagcagcatg
     2161 tgatagatgt ccaataaatg aatttacagt tacagagctt tgtagagggt gtttggctca
     2221 tagatgtaag gaagcatgta aatttggggc aatttcttat ataaatggga gagcatatat
     2281 aaatcatgat ttatgtaagg cttgtggaat gtgcaaaagt tcatgtcaat atgatgctat
     2341 atcagaagtt gtaagacctt gtaaaagtgt ttgtccaaca ggagccttgg attttaatag
     2401 aaacactatg aaagcaatga tacatgaaga taattgtata aattgtggag catgtatgtc
     2461 agcatgtcct tttggagcaa tatctgataa gagtttgata gcacctgttg caaaaaaact
     2521 tgcaaagaaa gaaaatatgt atgcaatagt agctccagct ataacaggtc aaatagatag
     2581 taatattaca tatggtcagt taaaaaatgc tataaaatca ctgggatttg tagatatgat
     2641 agaagcttct tgtggagcag atgcagttac agttcatgaa agcaatgagc ttattgaaag
     2701 aataaaatca ggagataatt acatgacaaa ttcatgttgt ccgggatttt ttaagtatat
     2761 agagaagatg ttcccagatg aaaaagatag aatatcatct acagtatctc ctatggtagc
     2821 aacaggaaga ttagtaaaaa aaatagattc tgatgccaaa gttgtattta tagggccatg
     2881 tacagctaaa aagagtgagg caacagagat aagtttaaaa gattcagtag attatgtact
     2941 tacttttgaa gaactaatgg cattatttga agcatttaat atagatttat caaaatgttc
     3001 aaatgaagat ataaatgatg gttcgatttt tggaagggga tttggagcat ctggtggact
     3061 tgcaaaatca gtacagaact atattgctga aaaggggctt agttttgatt ttgaacctat
     3121 aaaagtatct ggtccaagac aaattaaaaa gacaatgaca atggctaaat tgggaagatt
     3181 agaaggtaat tttatagaag gaatgatgtg tgaagaaggt tgtattaatg gagcaggtaa
     3241 gtttttatca ggtgtaaaat ctaaaaatac ttttgataga aaaaatggac aatctactaa
     3301 aaagagtgtt ttatctaatg ataagattaa agaatttgaa gatgtaaatt tagaaagata
     3361 attgttttgg tatagtcatt tttttatcat gccacaaata tgcctagaat ttgccctttt
     3421 tatatgtgta ttttacagag ttaagattgc tatactataa atatgtaata aataaaattt
     3481 ataggagctt ttggatgaag aaaataatat taaaagatat cctgttttta gttactacat
     3541 tatctataat agtaatatta ttgcttgtaa caaatataac tgctaatagt aagataaaat
     3601 caacaagtga aacctattta aaaagtgaca aaaatagtgt gagtcaaaat ggcatccaga
     3661 gtaacacaaa aaatactgca tttaagacta ctatactaag cactggaaag tcagattgta
     3721 tattagttga gataggtaac aaagtcataa tgattgatac aggagaagat aaaaatggaa
     3781 aacaaatagt tgctagatta aaagaaaaag gaataaatac acttgactat ttgattttaa
     3841 cccatctaga taaggaccat ataggtggag tagatagtgt gttatctagt gttaaggtaa
     3901 agaatattat acaagcaaat tataagaaag atagtaagca atatgatgaa tatattgact
     3961 cacttaaaaa agctgatata gaaccagtgc tactaaaaga taatatgaat attgtaatca
     4021 atagtgcaga aataaacata cagccggctt caaaaagcaa gtacgagagt tcaaatgatt
     4081 attcaataat aactaatata agctatggag cacataagtt tttatttgca ggagatgcag
     4141 aggaagaaag gttgtcagaa tttataaatg gaaatacctt aaaatatgat tttgttaaaa
     4201 tacctcatca tggaagatat gacaaattaa ctgaagcatt tttagagtct atttcacccc
     4261 aatgtgctgt tattacatgt tcagagaaaa aagaaccaga ggaaggcgtt ttaaaaatat
     4321 tggaaagatt gaatataaaa acatttttga cttctaatgg agaggtagtt atcaacagtg
     4381 atggtaagac attgagtgtg aatcaataat ttttttaaaa atatctaaaa aaatttaaaa
     4441 aaatcattag caaatattac cccttaaaca tagtatattt taggagctga aactccacat
     4501 acaaaatgac taggaggtaa catattatgt tggatagatt ttttggtgaa ggcggatttg
     4561 gtggatgtgc ttggtggata ataatattat tcttcttatt cttagcattc caagattgtt
     4621 gggctgaaat ggacttatgt gcatggatac ctttcttaat attattatta atactttgtg
     4681 aatgtggtga cgtttttgat ggaggatgtg gatgctaaaa aaatcaattt agatgattta
     4741 acaaagctta tggtgggtat aaacaaaata ccaaaccaat cacaatctag tgtttgaagg
     4801 tagtcggaaa tcccccgcta ccttcttttt tttattgtta aaatttattt ttgaattatt
     4861 tcatttacat ataataattt ttacgataaa gtctgatata attaaaaaga aaataaaatt
     4921 tatttatagg agatgtaaaa atggctatag tggataagat tttcaatgag ataaaactta
     4981 tggttgcaag acttttattg ggaaaaaaat acagtgaata tgaagaaaaa gatttagtgt
     5041 ataatacaga agaagaagtt atacttataa ctttaaaaag attagttttt cagggaaacg
     5101 taaatgaagc agaagaaatt ttatttgata aagctaagag tgtaaatagt gaaaatatgc
     5161 agtatatagc aatagaattt tatactatgc ttatggaaaa aactgatgaa gaattagaag
     5221 ctatgaattt ttccaaacaa gaagtttatc aaggtataga agatattaga aaagtattga
     5281 atttagaata aaagatatat gaaaatattg aaataaatta atgctaaggt gagaatatta
     5341 tatttatgta taaaaatata atattcttat tttattagtt atataagtat atcttattta
     5401 ccataaaact atcttgaaaa aaattaatct taattgtata atattacttt gagtgaaaaa
     5461 gtattaacat ttttttataa gatgttttac taaaaaaata tatataaata tactgttatc
     5521 aacaagaaat ttaatttagt gaggtgttaa gatgaaagta ggatttgacc acaaaaaata
     5581 tttagaagag cagtctaaat atatcttaga gagagtaaat aattttgaca aactttattt
     5641 agagtttgga ggaaaattga tgggagacct tcatgcaaaa agggtacttc caggatttga
     5701 tgaaaatgca aagataaaag tattacaaca tatgaaagaa aaagttgaag ttgtaatttg
     5761 tgtctatgca ggagatatag aaagaaacaa aataagagga gactttggta tcacttatga
     5821 tatggaagtt ttaagattaa tagatgattt aagaggttat gaactagatg taaatagtgt
     5881 agtaattact agatttgaag gacaacctgc tacaactgta tttataaata aattagaacg
     5941 tagaggaatt aaagtttata agcattatcc aacaaaggga tatccgtcag atgttgagac
     6001 tatagttagt gatgagggat atggagcaaa tgaatatata gaaactacaa agccaatagt
     6061 agtagtcaca gcaccaggac ctaatagtgg aaaacttggt acttgtctaa gtcagttgta
     6121 tcatgaaaat aaaagaggta atgtagttgg atattctaag tttgagacat tcccagtttg
     6181 gaatgtgcct gtaaaacatc ctttgaatat agcttatgaa gcagctacag ttgacttaaa
     6241 agatgtgaat atgatagatt cattccacct tgaaacttat ggtgaaatgt cagtaaacta
     6301 caatagagat ttggaattat ttccagtgct taaaaaaata atagaaaaga taactggaaa
     6361 agaatcaatt ttccaatctc caactgatat gggtgttaat agagttggtt ttggaataat
     6421 agatgatgaa gtaataagaa aagcttctat agaagaaata ataagaagat actttaaaac
     6481 tgcctgtgag tataaaaagg gacaagttga taaaggtgct tatgatagaa tgaaaatgat
     6541 tatggaagag cttaacttaa agccagagga tagaaatgtt gtattacctg ctagaaacta
     6601 cagtaataaa ttgaaagaaa gtgctgacaa gaatgatact tgccctgtag ttgcattaga
     6661 attagaagat ggtacaatat taactggtaa gggttctgat ttaatgaatg gaacagcagc
     6721 agtagtatta aatgccatta aacatttagc caatatatct gatgatatgc acttaatatc
     6781 acctgtaata ttagaaccta tcataaactt aaaaacaaag actttagcta gtagaaatgt
     6841 ttcacttagt tgtcaagagg tgttaactgc acttagtatt tgtgctgtaa ctaatccaac
     6901 tgcacaattt gctatggcaa agttagcatt attaaaaggg tgtcaagctc attccactac
     6961 tatattaaat agagatgatg aacaaacatt tagaaagctt ggtatagatg taacttgtga
     7021 cccagagtat ccttcacaaa atctttacta tagttaaaat tgtataaaaa aatatgggct
     7081 gttttaaagt aatagtttga gcagtccatt acaattataa taaaatcagt aggctgtctt
     7141 gtataagaca gtcttttgtt tttaaaacat cccataaatt aaatatatat actgttatcc
     7201 ataaggaatt ttaaatttag ttccttggaa tacctattgt aaaactttta tatagttttt
     7261 gcaagtgttt ttaaggattt tttataaatt atattgaagt taatggatta tagatagttt
     7321 tatttatgga aaatatgaat atataaagaa aataatgaaa aaataccaaa aagaaacata
     7381 agtttggtat aattaaaaag taatataaat aaaatttaat ctatttatac tttaaaaggt
     7441 gataatgatg aaacttcata gaaaaataat tcatatagat atggacgctt tttatgcatc
     7501 tatagaacaa agagataata aaaaattaaa aggaagacct gttattgttg gtggaaatcc
     7561 acaaagcaga ggtgtggttg caacgtgttc ttatgaagct agaaaatttg gtattcattc
     7621 agctatgcca tcagctgtag cttacaatag gtgtccatat gcagtttttg taagacctag
     7681 atttgatgtt tataagagtg tgtcagagca aataagagat atcttttata gatacacgga
     7741 tttagttgaa ccattatctt tagatgaagc ttacttagat gtaacaaaaa ataaaaaaag
     7801 tattgattca tcaattgaga tagcaaagca aataaaaaaa gatatattta gagaagttgg
     7861 tcttacttct tcagctggtg tgtcatacaa taagtttctt gctaagattg catcagactt
     7921 aagaaagcca aatgggttaa ctgtaataac cgaagaaaat gcccaggact ttttagataa
     7981 attacctgtg aataaatttt ttggtgttgg aaaagttact tcaaacactc ttaaaaattt
     8041 aggtataaaa actggatatg atttaaggtg tttaaattta tttgaacttg aaaatatatt
     8101 caaaaaaaga ggttatgaat tatataaatt tgctagagga atagacgata gaccagtgga
     8161 accaaatagg gttagaaaat ccgttggagc agagactaca ttaagtcata atttggatat
     8221 tgatgaagaa gaaacacgca acattcttga tgaactttgt gaagaagtat gtcatagatt
     8281 aaaaagctct gagaagtttg gaaaaacatt aacattaaaa attaaatatg aggattttac
     8341 aaagataact agaagcatta gtttagaaca ctatattgat gaatataatg atataagaag
     8401 tggtgtagat aatttgttga gaaatgttga ggttaatgga aaacaaatta ggctattggg
     8461 agtgacaata tcaaacttaa gcgataaaaa agaaacttat aaagatatta cattatttga
     8521 atacatggat agtatccaaa tgtgagtttt gtttaaatga aataacacta gctttaatac
     8581 tatataattg aggctagtgt tattttatga atatttttat actttttgtt ttttgacatt
     8641 atatattata tactatatat aaatacttta tatttgtagc aatattcaga caaatattga
     8701 cctaaaagtc gtactttgtt tacataaata aaatagtatg atataattat aaaaacaact
     8761 tataatattg gagaaggttg cttgttcgaa aaaggggatg ataaattatg atagagataa
     8821 gaaatgtaac aaaaaaaata ggaaataatg ttatattaga tgatatttct ctagttgtag
     8881 aaacaggtac attggtagtt ttgattggtt cgagtggttg tggaaagact actactttga
     8941 agcttataaa caaattaata aaacctacat ctggtgagat atatattaat ggcaaaccaa
     9001 tttctcagga aaatgaaata gagcttagaa gaaaaatagg atatgttata cagaacacag
     9061 gcctattccc tcatcttaca ataaaagaaa atatagagct aattccacgc ctaaaaaagg
     9121 aaaaatccgt tgaagaaatt gaaaaaagaa cgttgcaact acttgagatg gtaggacttg
     9181 atagtgatga atttttgaat aagtatcctt cagaacttag tggtgggcag caacaaagaa
     9241 tcggtgttgc aagagccatt gctactgatg ctgaaataat attgatggat gagcctttta
     9301 gtgctttaga cccaataacg agaacatctt tacaagaaca attattttct cttcaagatg
     9361 aattgaaaaa aactataata tttgtaactc atgatatgga tgaggcttta aagatagctg
     9421 ataaaatttg tattatgaag gatggaagaa tagcacaata tgatactccc gaaaatatac
     9481 ttagaaagcc tgcaaatgac tttgtaaaag attttatagg agaagataga gtttgggata
     9541 atccagaata tataaaagca agagatatta tgataaaaaa tcctattgct gttaattcta
     9601 ctagaactgt aactcaagga atagaaatta tgagaacgag taaggttgat agcttactaa
     9661 ttatagatag agcaaaaaca ttaaaaggta ttgttactgt aaaagatatg aaagatattg
     9721 atgataagtc aatattactt gctgatatta tgtcaagtga gcctttgcat gtaaatgaag
     9781 gtgataattt agtagaaata ctaaatgtaa tgaatcgtaa ttcggttgga tatattccag
     9841 ttatttctga tgaaaataaa ctggtagggc ttattactag aagtagtttg ctttctgttt
     9901 taagtgaaca attcttagaa atggaggtga gtgttcttgg ctaatttttt taactttata
     9961 ctattacaaa aagataagat tatagaactt ttaatacaac atatgagtct aactgttaca
    10021 tctattttaa tagctataat agttggtgtt cctcttggaa taattatttc aagaatctca
    10081 tctcttagaa agtttgtact tggatttgta aacttagtcc aagcagttcc gtctatggca
    10141 cttttgggtc ttcttgtacc tatacttggt ataggaagta aaccagccat atttatggta
    10201 gtggtttatt cattactacc aattgtaaaa aatacgtata taggtataac aagtattgac
    10261 ccagtagtat tagaatcagc taaaggtatt ggacttacaa gaaatcagac attatttaaa
    10321 atacaattcc ctttagcgct tcctataata atgggtggag ttagaatatc tgcagttaca
    10381 gctgttggtt tgatgaccct tgcagcattt ataggtgctg gtggtttagg atatttagta
    10441 ttttcagggg ttcaaactgt aaataataat atgattttag caggggcaat cccatcatgt
    10501 ataattgcac tgttagttga ttatttattt agtaaaattg aagttgcagt tactccaaaa
    10561 ggattaaatc caaaagcacc taagaaaaat tatatagcac ttaaagtaat atctgtagtg
    10621 cttgttgttt caatgttgtt cgttgttttt agttctagtt tttcaagtaa aaaagataca
    10681 ataactattg gttctaaaga ttatacagaa caacttatac ttggtaatgt ttatgctgaa
    10741 ttagtagaaa aaaatacaaa tcttaaagtt aagaagaatt taaatctagg tggttcatct
    10801 gttgcattta atgcaattaa atctggtgag ttagacatgt atgttgacta tacaggtaca
    10861 cttttggtaa atgtaatgaa acatgctccg attaaagatg ctgatgaagc atataatgtt
    10921 gtaaaagata ctatggaaaa agaaaatcag ttaactttac ttgaccctct tggatttaat
    10981 aatacttaca cattagccat gatgcctgaa actgctgaaa agtatggaat aaatactatt
    11041 tctgatttga ctaaatatgg taaagaattt acattctcac caactttgga gtttgaaaat
    11101 agagaagatg gtcttgtagg actttctaga gattatggtt taaaattcaa agatgtaaaa
    11161 gcaatgaatg gaagtttaag atatacagct ttagataata atgaatctca ggttatagat
    11221 gcatttttaa ctgatggtct tattaaaaag tttaatctta agatattaga ggacgataaa
    11281 aacttctttg taccatatta tgcagcacca ttagtgagag aagatacttt gaagaaatat
    11341 cctgagttag aaaaagttat gaatatgctt tcaggaaaag taaatgaaga aactatgaga
    11401 gaacttaact atcaagtgga tgaacttggt aaatcaccag aagaagttgc tcattcattc
    11461 ttagttaaag aaggcttagt ataaaaaatt aatagtttat atcaaaaatt taagggagtg
    11521 tctcaaaatg aactttttag ttcatgaggc actctttttt atatgaaatc aaatatattt
    11581 tatcatttca acaataaaaa agaagcttat ctttattttg agacagtctc ttatttttat
    11641 gtataccaaa accataccta cgatttttat ttataattaa gtttcaaata gaatgaacaa
    11701 gtacaaccaa ttttgaatat aaatatagta aatgtgctta tatacgattt tattgtctgg
    11761 ttgattgata ttgatagata gctttcttta ttgaatcgaa gggaggggga atttgttatg
    11821 gataactata agaaagtgaa gcaagtattg tggataattt tatttgctaa ttttgcagta
    11881 gcattattaa aaatcattat tggtaatcaa ataaaaagct atagtatgac agcagacgga
    11941 tttcattctt tgtcagatgg agcatctaat atagtagggc ttataggtat tttctttgca
    12001 tctaaaccaa aagataaaaa tcatccttat ggacataaaa agtttgaaat tataaccagt
    12061 ttatttataa gtggtatgtt gtttgttata gcaattaaga taattcttag tgcagtgtta
    12121 agaataacta atccagttgt acctgctata actatagaaa gtttaattgc tttaatcatt
    12181 acactcttta taaatatttt tgtatgtatg tatgaatata gagttgggac taaactaaat
    12241 agttatgttt tgatttcgga ttcattgcat acaagaagtg atatatttgt gtctctaggt
    12301 gtattggtta ctttagttgg tgttaagtta ggatttccag taataataga atctatagtt
    12361 cctattatta tttctgcgtt cataatttat tcggcttatg gaatatttag gccatctatt
    12421 ggaattttgg tcgatagagt ggctgtagat gaggattata taaaagaaat tgtgtttgaa
    12481 tttaatgagg taagggatgt tcacaatatt agaagcagag gtagcaaaag ttctatttat
    12541 attgacatgc atgtaatggt agacccattt ataagcgtag agcaatctca tgatttaaca
    12601 cataaaattg agaaacaaat acaagaagaa attaatgaaa atgctcaagt aatagtccat
    12661 attgaacctt tttatagttt tt
//
LOCUS       DAEPXF010000107        12449 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain HB27
            SAMN13376389-rid12479423.denovo.113, whole genome shotgun sequence.
ACCESSION   DAEPXF010000107 DAEPXF010000000
VERSION     DAEPXF010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13376389
            Sequence Read Archive: SRR10518432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 12449)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12449)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001069624.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001069624.1
            Genome Coverage       :: 315x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/15/2021 01:54:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,165
            CDSs (total)                      :: 4,076
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 89
            rRNAs                             :: 10, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 10 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 19 of 69
            Pseudo Genes (incomplete)         :: 44 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 10 of 69
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12449
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13376389-rid12479423.denovo.113"
                     /strain="HB27"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="China: Hebei"
                     /collection_date="2017"
     gene            complement(97..783)
                     /locus_tag="KRL67_002578"
     CDS             complement(97..783)
                     /locus_tag="KRL67_002578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HBH1523446.1"
                     /translation="MFGVIANSLAIAGGCIVGLIIKGGLPQRVSDTIMNGIALCVLYI
                     GISGVLEGKNTLVTIISVAVGALIGELIDIDKWVNKLGAFLQSKFSKGNKKDSIAEGF
                     ISSSLLFCIGAMAVVGSLESGLTGSHDTLFVKSVIDGIASIIFTASLGIGVMFSAISV
                     FLYEGIICVGASFLNGFLSDPVVTEMTAAGSLLIIGLGLNVLKLTNIKVANLLPAIFI
                     PILFGVFGII"
     gene            complement(832..1365)
                     /locus_tag="KRL67_002579"
     CDS             complement(832..1365)
                     /locus_tag="KRL67_002579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA synthetase associated
                     domain-containing protein"
                     /protein_id="HBH1523447.1"
                     /translation="MSDLNNKKEQDENESSMNETKIYDILDKLNVEYDVVEHEAVYTA
                     EQLVILDGITKGCQCKNLFLRNAKGNKYYLIVVKGEKQVDLNSLKDKIGSSRLSFASP
                     ERLYNVLKLLPGSVNPFSLINDTERKVELYIDKDVIKEDYLNFHPNINTKTVNISRKG
                     FEEFLEYLKYPIQSIDV"
     gene            complement(1409..2041)
                     /locus_tag="KRL67_002580"
     CDS             complement(1409..2041)
                     /locus_tag="KRL67_002580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HBH1523448.1"
                     /translation="MKVFDLTHVTHNDMPVYAEPNRPDIKKVAIIEENGYQETLISVF
                     SHNGTHMDSPRHMYTKGETLDKLDIENFVGKAYVLELEKGNENIELEYLKKYEDEIKN
                     SDFIIFKSGWSKFWDKKQYYVGYPTLTKEAANYIANTNIKGIGIDMLSVDRYDTSVFE
                     VHHILFEKGKIIIENLTNLENVPEKFLFIAAPFKYNDADGAPVRAIAIVE"
     gene            complement(2128..3210)
                     /locus_tag="KRL67_002581"
     CDS             complement(2128..3210)
                     /locus_tag="KRL67_002581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIR2 family protein"
                     /protein_id="HBH1523449.1"
                     /translation="MKIAIFLGAGASAAENLPIQNELFSEYFKKLNPTNYNTKMNREL
                     YIFFKQMFNIDIVKDNIDNANFPTFEEVLGLLDLAEQRKEAFRNFGLENLNNRSDSIR
                     FLRQYLILLMAEAIHNTPRTDNKYHKLLVENLLENDLLLDTTFISANYDIHIDNTVTG
                     LRDKNDLPIMLDYGVEFTNFKEELNWRKPKEPLIKLYKIHGSLNWLYCPICNSVTLTP
                     HEGGVMKLIENSSETKCLECGELTEPIIVPPTYFKNMSNIFLSNVWNETEKTLRDTDL
                     LIFCGYSFPEADMHIKYMLKRVQTNRKKPPLKIMVFNNHSQKQRITLKKEEGRYKRFL
                     GEDVIFTDNSFQDFSVNPLRFIKNIY"
     gene            complement(3343..4689)
                     /gene="pepV"
                     /locus_tag="KRL67_002582"
     CDS             complement(3343..4689)
                     /gene="pepV"
                     /locus_tag="KRL67_002582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepV"
                     /protein_id="HBH1523450.1"
                     /translation="MKEQIKEKVNSLQDEMISSIQESVKIPSVISEATENCPFGENVD
                     KALRGILDLCKSLGFKTVYKDGYYGYAEIGQGEKMIGILGHVDVVPEGDLESWNYPPF
                     EAVLEDGKLYGRGTQDDKGPTISAIYAVKALMDLNVDFNKRIRFIFGADEENLWRCIN
                     KYKENNEEIPNYGFTPDSRFPITNAEKGLLQVHLTCDSKSDIELSVGKALNAVPGKAI
                     YIGKYSDKLKKELDKLNFEYTVEGNKICIMGKSVHSAASDTGINAVARLCIALNNIGI
                     DSNIIKFLAEVIGEDANGNNIIPNCKDDVSGKLTVNIGRVTIDSEKEFAGIDVRIPVT
                     YKKDDFVKELKKMTDKYNLNYEEYDFLDSIYVPEDTLLVKTLRKVYEEETGLDGTPLS
                     SGGATYARALDNCVAFGAIFPGKPETEHQANEYLIVEDIIKATQIYALSIYELLKI"
     gene            complement(4758..6038)
                     /locus_tag="KRL67_002583"
     CDS             complement(4758..6038)
                     /locus_tag="KRL67_002583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine gamma-lyase family protein"
                     /protein_id="HBH1523451.1"
                     /translation="MLNETKELLKDYYGIDDDTFKLSQEIMEDIKDKFEEIKEIREYN
                     QYKVLKAMQESKLSDMHFNWTTGYGYNDIGREKIEEIYSKVFNTEDALVRPIIVNGTH
                     ALTLCIQGIVRPGDEILSVTGRPYDTLEGVIGIREEKGSLKEYGVTYDDLDFLEDGNL
                     DLEGIKNKINDRTKLVMIQRSKGYSWRKSLSISDIKEAIEVIKSVKPEAIVMVDNCYG
                     EFLDTKEPTDVGADVMAGSLIKNPGGGLALTGGYIAGRKDLIELISYRMTSPGIGKEC
                     GLTFGTTRNVLQGFFLAPYIVSQAVMGAIFCSRAFEKLGYDVLPKYNDLRSDIIQCIR
                     LNNADEVISFCEGIQEAAPVDSYVKPVPWDMPGYESEVIMAAGAFIQGSSIELSADAP
                     IRPPYNVYFQGGLTFDHSKMGTLKAIEFIKKLKK"
     gene            complement(6165..6425)
                     /gene="hfq"
                     /locus_tag="KRL67_002584"
     CDS             complement(6165..6425)
                     /gene="hfq"
                     /locus_tag="KRL67_002584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HBH1523452.1"
                     /translation="MKNTVLNLQDLFLNNARKERIPVTIYLVNGVQVKGLVKGFDSYI
                     ILIEGDNRQQNMIYKHAVSTIQPGKYINLTNQNQNNNNNNNR"
     gene            complement(6539..7474)
                     /gene="miaA"
                     /locus_tag="KRL67_002585"
     CDS             complement(6539..7474)
                     /gene="miaA"
                     /locus_tag="KRL67_002585"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HBH1523453.1"
                     /translation="MKKIPLIILTGPTAVGKTDLSIKLAKDMDAEIISADSMQIYEYM
                     DIGSAKVTEEEMQGVKHYLIDEVKPDYPFSVSEFQQRAKKYIYEINEKGKCVLVTGGT
                     GLYLNSLIYNMDFAQSDANNELREELQNQLAEKGIDYMHNKLKELDEESANRIHKNNT
                     KRVIRALEVCLSGKKMNDFSSDLKFNEEYQPIIIVLNRDREHLYQRINMRVDIMLKNG
                     LVEEVKKLLSMGFKKDMISMQGIGYKEILKYLDGEYTYEEAIEIIKRDSRRYAKRQIT
                     WFKRYKTAKWFDLDQYENIDELKNEIILYIKDSIK"
     gene            complement(7512..9479)
                     /gene="mutL"
                     /locus_tag="KRL67_002586"
     CDS             complement(7512..9479)
                     /gene="mutL"
                     /locus_tag="KRL67_002586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HBH1523454.1"
                     /translation="MKNIINILDDLTINKIAAGEVVERPSSVVKELIENSIDAGANKI
                     SIDIIDGGKSLIKITDNGIGIPSSEVEKSFLRHATSKIKKIDDLYDLYSLGFRGEALA
                     SISAVSKLEMMTKTKDEMIGTKIYVEGGKIITKEPIGSTNGTTIIIKDIFFNTPARQK
                     FLKSTHAETINISDLINKLAIGNPNIQFKYTNNNKQMLNTPGDGKLVNTIRSIYGKEI
                     TENIIDVEFKCNHFKMNGYIGNNNIYRSNKNLQHIYINKRFVKSKIIIDAITESYKSI
                     IPIGKHAVCFLNIEVDPSCIDVNIHPNKLEIKFEKEQEVYIELRDFLKVKLIHSNLIG
                     KYATYSDKKTQPRIAINSREKSTDYKLRNNDLLESTPKNSNITKGNDEVIEVVTLSSE
                     KPINEFQSVSEVLNASVEDDVKNINYLSEDSANDNIQEEFQVDGIKNEGNYYLGDSIK
                     DSEEEYLCSSKRKFSLYGYSVIGVVFNTYIILSKDDSMYLLDQHAAHERILYERYMEK
                     FYRQDINMQILLDPVVIEVSNVDMLQIENNLELFMKFGFELEIFGNNHIMVRCVPTIF
                     GVPETEKFILQIIDNIEEITSNYDLKGERFASMACRSAIKANDKIYDIEIKSLLEQLE
                     KCNNPFTCPHGRPIMVEISKTEIEKMFKRIM"
     gene            complement(9490..12333)
                     /gene="mutS"
                     /locus_tag="KRL67_002587"
     CDS             complement(9490..12333)
                     /gene="mutS"
                     /locus_tag="KRL67_002587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HBH1523455.1"
                     /translation="MQIDMNKLTPMMKQYLEVKNRYKDCILFFRLGDFYEMFFEDALV
                     ASKALEIALTGKACGLEERAPMCGVPFHSANSYISKLVENGYKVAIGEQMEDPSTAKG
                     IVRREVIRVITPGTVLDGNLLENKKNNYLLSLYKDGTNIGLTYVDISTGETNATCLNE
                     DKVIEEIAKIHPTEIIINDLDFIEKLRDIATVSNIYINESFSDNYLDINILKEYFPDV
                     YLQKLKFDDKGLIKSSLSILLNYIYNTQKQITSNINNINIYNSSEYMVLDMFTRTNLE
                     LTQTIRGNKKKGSLLHVLDKTSTAMGGRLLRKYVEEPLINKSKIENRLDVIEEIKDDF
                     ILREDLNDILKNIYDIERICGKIAFERVTPKELIHLKNSIEKLPNLKDTINLSNAKIL
                     KKYVSEMDKLDDIYNLIDEAILEEPTITIKDGNIIKSDFSDELKELREISKNGAFLVK
                     EIENREREKTGVKSLKIGFNKVFGYYIEITKANFKQAKLDETYIRKQTLSNAERYITP
                     ELKEIEEKILHAEEKIKSLEYEIFVEIRDTIYKNIDRIQKVAKIIANIDVFVSLATVA
                     HINNYVKPAINENNKLDIRNGRHPVVENIVGEENFVPNDTYLNRGENIINIITGPNMS
                     GKSTYMRQTAIIALMAHIGSFVPAESADIPILDRIFTRVGASDDLSQGQSTFMVEMNE
                     VSLILKNATERSLVILDEIGRGTSTYDGISLAWSIVEYIQKNIRCKTLFATHYHELTD
                     LEEEFKEVKNYSIAVKEDGEGIIFLRKIIPQGADKSYGIYVAKLAKLPDEVIERAKYI
                     LKDLEKNHVYNSVAINGDKENNNIINSNLDEELVKVNQSNFKNKYESLKIEHELIVKD
                     YKHIKKDYDKLNSKFKALNDEVALMKQNDDKEKINQEDSVKEVALTQISFDSVNRDIL
                     SEEILNLDILNMTPLDAINSLYNLQKKAKEIKS"
BASE COUNT         3851 a         2162 c         1276 g         5160 t
ORIGIN      
        1 taattattaa tttgatgata aaagctattt tccttctaaa taaaaaattt gtctaaagtt
       61 tctaaaaaat ttagacaaat cttatgttat tactctttaa attattccaa atacgccaaa
      121 taatattggt ataaatatag ctggtaataa attggctact tttatattcg ttaatttaag
      181 tacatttaga ccaagtccta taattaataa acttccagct gctgtcattt ctgtgactac
      241 aggatcactt aaaaatccat ttaaaaatga tgctcctaca catataatac cttcatacaa
      301 aaatacagat attgcagaaa acattacacc tatacctaaa gaagcagtaa atataattga
      361 agctattcca tctataacag attttacaaa taaagtatca tgacttcctg taagaccact
      421 ttctaaggaa ccaactactg ccatggctcc tatacagaat aataaactag atgatataaa
      481 accctctgct atagaatctt ttttatttcc tttagaaaat ttactttgta aaaaagcacc
      541 caacttattt acccatttgt caatatctat taattctcct atcaaagcac ctacagctac
      601 ggatattatt gtcactaatg tattttttcc ttctagtact cctgatatac caatgtacaa
      661 tacacataag gcaattccat tcattatagt atcacttact ctttgaggta atcctccttt
      721 tattataaga cctactatac atccacctgc aattgctaga ctatttgcta ttactccaaa
      781 cattttttct cccctcgatt agctaattga aatttatcct gctattaaaa cctaaacatc
      841 aatactttgt attggatatt ttaaatattc caaaaattct tcaaaacctt ttctagatat
      901 atttacggtt ttagtattta tgttaggatg aaaatttaag taatcttctt taattacatc
      961 tttatctatg taaagctcaa cttttctttc tgtatcattt attaatgaaa aaggatttac
     1021 acttccagga agaagtttaa gtacattgta tagtctttca ggtgaagcaa acgataatct
     1081 agaacttcct atcttatctt taagtgagtt taaatcaact tgtttttctc ctttaactac
     1141 aataagataa tacttatttc ctttggcatt tcttaaaaat aaatttttac attgacaccc
     1201 ctttgttatt ccatctaaaa tgactaattg ctctgctgta tatacagctt catgctctac
     1261 tacatcatat tcaacattta atttatccaa aatatcatat attttagttt cattcatgct
     1321 agattcattt tcatcttgtt cttttttatt atttaaatca ctcatacttt tctccttaaa
     1381 cacttcatta taatatcatt gtgagatatt attctactat agctattgct cttactggag
     1441 ctccatcagc atcattatac ttaaatggtg ctgctataaa tagaaatttt tctggaacat
     1501 tttctaaatt tgtaagattt tctatgatta tttttccttt ctcaaataaa atatgatgca
     1561 cttcaaatac tgatgtgtca tatctatcca ctgatagcat atctattcct atacctttta
     1621 tattagtatt agctatatag tttgcagctt cttttgttaa tgtaggataa cctacatagt
     1681 actgcttttt atcccaaaac tttgaccaac ctgatttaaa tattataaaa tcactatttt
     1741 ttatttcatc ttcatatttt tttagatatt ctaattctat attctcattt cccttttcaa
     1801 gttccaatac ataagctttc ccaacaaaat tttctatatc caacttatct agggtctcac
     1861 ctttagtata catatgtctt ggtgaatcca tatgagttcc attgtgagaa aatacactaa
     1921 ttaatgtttc ttgataacca ttttcttcaa taatagctac tttcttgata tctggtctat
     1981 taggctctgc atatacaggc atgtcattat gtgtcacatg agttaaatca aaaaccttca
     2041 tctttatata ccccctatgt ttttattttc tattatatac ctaaattcat tttatttcta
     2101 tcagatttta ttccattata taccatacta gtatatattt tttataaacc tcaagggatt
     2161 taccgaaaaa tcttggaaag aattatcagt aaatataaca tcttcaccca aaaaacgttt
     2221 ataccttcct tcttctttct ttagagtaat tctctgtttt tgtgaatgat tattaaaaac
     2281 cattatctta agaggaggtt tttttctatt agtctgcact ctttttaaca tgtattttat
     2341 gtgcatatca gcttcaggaa atgaatatcc acaaaatatt aaaaggtcag tatctcttaa
     2401 agttttttct gtttcattcc aaacattact taaaaatatg tttgacatat tcttaaaata
     2461 tgttggtggc actataatag gttctgttaa ctcaccacac tctaaacatt ttgtctctga
     2521 actattttca ataagcttca tgacgccacc ttcatgtggt gtaagagtta cactattaca
     2581 aatggggcaa taaagccaat tcaatgaccc atgtatctta tataatttga ttaatggttc
     2641 ttttggtttt ctccaattta actcctcttt aaaattggta aactccacac cataatcaag
     2701 cataattggc aaatcattct tatctcttaa tccagtaact gtattgtcta tatgtatatc
     2761 ataatttgca ctaataaaag tagtatcaag aagcaaatca ttttccaaaa gattttcaac
     2821 taaaagttta tgatatttat tatctgttct aggtgtatta tgaattgctt cagccattag
     2881 cagtattaaa tattgtctta aaaaacgtat actatcactt ctgttattta gattttctag
     2941 tccaaaattt ctaaatgctt cttttctttg ttctgctaag tctaaaagac ctaaaacttc
     3001 ttcaaaggta ggaaaatttg cattatctat attatctttt actatatcaa tattgaacat
     3061 ctgtttaaag aaaatataca attctctatt cattttggta ttataatttg tgggatttag
     3121 ttttttaaaa tattcgctaa aaagttcatt ttgaataggt agattctctg ctgctgaagc
     3181 tcctgctcct aaaaatattg ctattttcat atagttcctc ctaatataaa tattactcct
     3241 attttcatat ccattaaata tccatatcat ttattttatg atacaacaat aaagctgtct
     3301 tttactagaa aaaatcctat tacaagacag ccttatatta tattaaatct ttaataattc
     3361 gtaaatagaa agagcatata tttgagtagc ttttataata tcctctacta ttaagtattc
     3421 attagcttgg tgttctgttt ctggttttcc tggaaaaata gctccaaatg caacacaatt
     3481 atctaaagct cttgcgtaag ttgctcctcc agatgaaagt ggtgttccat caagaccagt
     3541 ttcttcttca tatacttttc taagtgtctt aacaagaaga gtatcttctg gaacatagat
     3601 tgaatccaag aaatcatatt cttcataatt taaattgtat ttatctgtca ttttctttaa
     3661 ctcttttaca aaatcatctt ttttgtatgt tactggaatc cttacatcta ttcctgcaaa
     3721 ttctttttcg ctatcaatag ttactctacc tatatttact gttagtttac ctgatacatc
     3781 atccttgcaa ttaggaatta tattgtttcc attagcatct tcaccaataa cttctgctaa
     3841 aaacttaatt atattagaat ctatacctat attattaaga gctatacaaa gtctagccac
     3901 tgcatttata ccagtatcac ttgctgctga atggacactt tttcccatta tacagatttt
     3961 attaccttca acagtatact caaaatttaa tttatcaagt tcttttttta atttatcaga
     4021 gtattttcca atatagattg ctttacctgg aactgcattt aaagcctttc caactgacaa
     4081 ctctatatca cttttgctat cacaagtcaa atgaacttgt aaaagacctt tctctgcatt
     4141 tgttattggg aatctagaat ctggagtaaa tccatagttt ggtatttctt cattattttc
     4201 tttatattta tttatacatc tccaaagatt ttcttcatca gccccaaaaa tgaatcttat
     4261 tctcttatta aagtctacat ttaaatccat aagagctttc acagcataaa ttgctgatat
     4321 tgtaggtcct ttatcatctt gtgttcctct tccatacaat ttaccatctt ctaaaacagc
     4381 ttcaaatgga gggtaattcc agctttctaa atcaccttct ggaaccacat caacgtgacc
     4441 aagtatacct atcatttttt caccttgtcc tatttctgca tatccataat atccatcttt
     4501 gtatactgtc ttaaatccta gagatttaca taagtctaat atacctctta aagctttatc
     4561 tacattttca ccaaatggac aattttcagt tgcttcagat ataacactag gtatttttac
     4621 actttcttgt attgaagaaa tcatttcatc ttgtagagaa tttacttttt ctttaatttg
     4681 ctctttcata ttatcaccct tcaattttca tcataatata ctaataaata ttttgacctt
     4741 ttaaaaaaaa aatatgctta ttttttcagt ttttttataa attcaatcgc ttttaaagtc
     4801 cccatttttg aatggtcaaa tgtaagacca ccttggaaat aaacattgta aggaggtctt
     4861 attggagcat ctgcacttaa ttctatagac gaaccttgta taaatgctcc tgctgccatt
     4921 atgacttcac tttcatatcc aggcatgtcc caaggaacag gttttacata agaatcgact
     4981 ggtgcagctt cttgtatacc ttcacaaaaa cttattactt catcagcatt attaagtcta
     5041 atacattgaa taatatcact tcttaagtca ttgtattttg gtaatacatc atagcctaat
     5101 ttttcaaaag ctcttgagca aaagatagct cccattacag cctgagatac tatataaggt
     5161 gccaagaaaa atccttgaag tacatttcta gttgttccaa aagtaagacc acattctttt
     5221 cctattccag gggaagtcat tctataagat ataagttcaa tcaaatcttt tctaccagct
     5281 atatatccac ctgtcaatgc aagcccacca ccaggatttt ttattaaaga accagccata
     5341 acatctgctc cgacatcagt aggctctttg gtatctaaaa actcaccata acaattatca
     5401 accattacta tagcttctgg tttaacagat tttattactt ctatagcttc ttttatatca
     5461 ctaattgaaa gtgattttct ccaagaatat ccttttgacc tttgtatcat aacaagttta
     5521 gttctatcat ttattttgtt tttaattcct tctaaatcta agtttccatc ttctaaaaaa
     5581 tctaaatcat catatgtaac accatactct ttcaatgaac ctttttcttc tcttatgcct
     5641 ataactccct ctaaagtatc atatggtcta ccagtaacag ataaaatttc atctcctggt
     5701 ctaactatac cttgtataca taaagtaagt gcatgtgtac catttacaat tattggtctt
     5761 acaagagcat cttctgtatt aaatacttta gaataaattt cctctatttt ttcacgacca
     5821 atatcattat atccatatcc tgtagtccag ttaaagtgca tatcacttag tttagattct
     5881 tgcatagctt ttaaaacttt atattgatta tattctctta tttcttttat ttcttcaaac
     5941 ttatctttta tatcttccat tatttcttgc gatagtttaa aagtatcatc atctattcca
     6001 tagtagtctt ttaataattc ttttgtctca ttaagcattt tatcacctgt ctctttatta
     6061 ttttatttaa acatgtaaag gagactattt tactaaaaca aataaattat agtttataag
     6121 ttagtataag tctcctctca atcatcttaa attaaattaa ttatctatct gttgttatta
     6181 ttattgttgt tttgattttg atttgttaga ttaatatact ttccaggttg tatagttgat
     6241 acagcatgtt tataaatcat gttttgttgt ctattatccc cttctattaa tattatataa
     6301 ctatcaaacc cctttacaag ccctttaact tgcactccat taactaaata tatagtaaca
     6361 ggtatccttt ctttccttgc attatttaaa aacaaatctt gtaaatttaa aactgtattt
     6421 ttcattagat ttgtaccctc ccaagattaa attgaacttc taaaatattc ctaccttata
     6481 ttctatataa gttatgacaa attttcaata ttaagttgta tacttaaaaa tttatatatt
     6541 attttataga atctttaata tataagatta tttcattttt taattcgtct atattttcat
     6601 attggtctaa atcaaaccat tttgctgttt tatatctttt aaaccatgtt atctgacgtt
     6661 ttgcatacct tctagaatct cttttaatta tttcaatagc ttcttcgtat gtatattctc
     6721 catccagata ctttaatatc tctttataac ctataccttg cattgatatc atatcttttt
     6781 taaatcccat acttaaaagt ttttttacct cttcaaccaa cccattttta agcataatat
     6841 caactctcat atttattctt tgatacaaat gttctctatc tctatttaga actattatta
     6901 ttggttgata ttcttcatta aattttaaat cacttgaaaa atcattcatt ttcttaccac
     6961 ttagacaaac ttcaagagct cttatgaccc ttttagtatt gtttttatgg attctattag
     7021 cagattcttc atcaagttct tttaacttat tatgcatgta atctattccc ttttcagcaa
     7081 gctgattttg aagttcttct cttaattcat tattggcatc agattgtgca aaatccatat
     7141 tatatattaa agagtttaaa taaagtcctg tgcctccagt tactaaaaca cattttccct
     7201 tttcatttat ttcatatata tatttttttg ctctttgttg aaactctgaa actgaaaatg
     7261 gatagtcagg tttaacttca tctataaggt aatgcttcac gccttgcatc tcttcctctg
     7321 taacttttgc acttccaata tccatatatt catatatttg catagaatca gctgaaatta
     7381 tctctgcatc catatctttt gctaatttta ttgacaaatc tgtttttcca acagcagttg
     7441 gacctgtaag tattataagt ggtatttttt tcataacatt cctttccata cattttaaat
     7501 atccaaatat tttacataat tcttttaaac attttttcta tttccgtctt agatatttcg
     7561 accattatag gtcttccatg aggacatgta aatggattat tacacttttc tagctgttcc
     7621 aatagacttt ttatttcaat atcatatatt ttatcatttg cttttatagc agacctacat
     7681 gccatagatg caaatctctc tccttttaaa tcataattac ttgtaatttc ttctatatta
     7741 tcaattattt gaagtataaa tttctcagtt tcaggtactc caaatatagt aggaacacat
     7801 cttaccatta tatgattatt gccaaaaatt tctagttcaa aaccaaattt cataaataac
     7861 tccaagttat tttcaatttg aagcatatct acatttgata cttcaataac cactgggtct
     7921 aaaagtattt gcatattgat atcttgtcta tagaacttct ccatatatct ttcatatagt
     7981 attctttcat gtgcagcatg ttggtccaac aaatacatag aatcatcttt gctcaaaatt
     8041 atataagtat taaatacaac tccaattaca gaatatccat ataaggaaaa cttgcgtttt
     8101 gaactacaca agtattcttc ttcactgtcc ttaatactat ctcctaagta ataatttcct
     8161 tcatttttaa ttccatcaac ttgaaactct tcttgtatat tatcatttgc actatcttca
     8221 cttagataat taatgttttt tacatcatct tctacacttg catttaaaac ttcacttact
     8281 gattgaaatt catttattgg tttttcactt gataaagtca caacttcaat aacttcatca
     8341 ttacctttag taatattact atttttagga gtagattcta ataaatcatt atttcttaat
     8401 ttataatctg tacttttttc tctactgtta atagcgattc taggttgtgt ttttttatca
     8461 ctataagtag catatttccc aataagattt gaatgtatta acttaacctt taaaaaatct
     8521 ctgagttcta tatacacttc ttgttctttt tcaaatttaa tttcaagttt attggggtgt
     8581 atatttacat ctatacatga tgggtctact tctatattta aaaagcaaac tgcatgttta
     8641 cctattggaa ttatagactt ataactttct gttattgcat ctatgattat tttacttttt
     8701 acaaatcttt tatttatata gatatgttgt aagtttttat tagaacggta tatattgtta
     8761 ttacctatat atccattcat tttaaagtga ttgcacttaa attctacatc tattatattt
     8821 tccgttattt cttttccata tatacttcta atggtgttta caagttttcc atcccctgga
     8881 gtatttagca tctgtttatt gttatttgta tatttaaatt gaatgtttgg attacctatt
     8941 gcaagtttat ttatcaaatc acttatgttt atagtttctg catgagtaga ttttaaaaat
     9001 ttctgtcttg ctggtgtatt aaagaaaata tcttttataa tgatggtagt tccattagta
     9061 gaacctattg gttcttttgt tattattttt ccaccttcaa catatatttt agttcctatc
     9121 atttcatctt tagttttagt catcatttct aattttgata cagctgaaat agatgctaat
     9181 gcttctcctc taaaaccaag agagtataaa tcatacaaat catcaatttt tttaatttta
     9241 ctagtagcat gtctcaaaaa tgatttttca acttcacttg aaggtattcc tattccatta
     9301 tctgtaatct taattagact ttttcctcca tcaattatat ctatggaaat tttatttgca
     9361 cctgcatcaa tggagttttc aataagttct tttactacag aagaaggtct ttctacaact
     9421 tcacctgctg ctatcttatt tatagtcaaa tcatctagga tgttaattat atttttcatt
     9481 tttacacctc taagatttta tttcttttgc ttttttttga agattataaa gagagtttat
     9541 agcatctaat ggagtcatgt ttaaaatatc cagatttaaa atttcttctg atagtatatc
     9601 tctatttaca ctatcaaaag agatttgagt taaggctact tcttttacag aatcttcttg
     9661 attaattttc tctttatcat cattttgctt cattaaagca acttcatcat ttaatgcttt
     9721 aaatttagaa tttaatttat cataatcctt ttttatatgt ttgtagtctt ttactataag
     9781 ttcatgttct atttttagag attcatattt attcttaaaa ttactttgat ttaccttaac
     9841 taattcttcg tctaaattac tattaattat attattattt tctttatctc catttattgc
     9901 aacactgttg taaacatgat ttttttccaa gtctttaagt atatattttg ctctttctat
     9961 gacttcgtct ggtaatttag ctaatttagc aacatatatt ccataactct tgtctgctcc
    10021 ctggggaatt atttttctta aaaatattat tccttcccca tcttctttga ctgctattga
    10081 ataattttta acctctttaa actcttcttc taaatcagta agttcatgat aatgtgttgc
    10141 aaataatgtc ttacatctaa tatttttttg aatatattcc actattgacc atgctaaact
    10201 tatcccatca taggtactag tcccacgacc tatttcatct aatataacta aacttctctc
    10261 tgttgcattt tttaatataa gggatacttc gttcatctct accataaaag tagattgacc
    10321 ttgagataaa tcgtcactag ctccaactct tgtaaatatt ctatctaata ttggaatatc
    10381 agcagactct gctggtacaa atgagcctat atgtgccata agagctatta tagcagtttg
    10441 tctcatatat gttgatttac ctgacatatt agggccagtt attatgttaa taatgttttc
    10501 tccacgattt aaataagtat catttggaac gaaattttcc tctcctacta tattttcgac
    10561 aacaggatgt ctaccatttc ttatatcaag tttattattt tcatttattg ctggttttac
    10621 atagttattt atatgtgcaa ctgttgcaag agacacaaaa acatctatat tagctataat
    10681 cttagctact ttttgtattc tatctatgtt tttatatatt gtatctctta tctctacaaa
    10741 tatctcgtat tctaaagatt taatcttttc ctctgcatgt agtatctttt cttctatttc
    10801 ctttaattct ggagttatat atctttctgc attactaagt gtttgctttc ttatatatgt
    10861 ctcatctagc tttgcctgtt taaaattggc ttttgttatt tctatataat acccaaaaac
    10921 tttattaaat cctattttta atgatttaac tcctgttttt tctctttccc tgttttctat
    10981 ttcctttaca agaaaggctc cattcttaga aatctctctt agttccttaa gttcatcact
    11041 aaaatctgat ttaattatat ttccatcttt tatggttatt gtaggttctt ctagaatagc
    11101 ttcatctatt aaattgtata tatcatctaa tttatccatt tctgaaacat acttttttag
    11161 gatttttgca tttgataaat ttatagtatc ttttaaatta ggtaactttt ctatcgaatt
    11221 ttttaagtga attaactctt ttggtgttac tctctcaaat gctatctttc cacatattct
    11281 ttctatatcg tatatatttt taagtatatc atttaggtct tctcttaata taaagtcatc
    11341 ttttatttct tctattacat ccaatctatt ttcaatttta gatttattta tcaaaggttc
    11401 ttctacatat tttcttaaaa gtctacctcc cattgctgta gaagttttat caagtacatg
    11461 caacaatgaa ccttttttct tatttcctct tatagtttga gtaagttcta aattagttct
    11521 agtaaacata tctaaaacca tatattctga tgaattgtat atatttatat tattaatgtt
    11581 agacgttatt tgtttttgag tattgtatat ataattcaga agaattgata agcttgactt
    11641 tattaggcct ttatcatcaa actttagttt ttgtagataa acatctggaa aatattcttt
    11701 taatatattt atatctaaat agttatcact aaaactttca tttatgtata tattacttac
    11761 agtagctata tctctaagct tttctataaa gtctaaatca tttataatta tttcagttgg
    11821 atggattttt gcaatttctt caattacctt atcttcattt aaacatgttg cattagtttc
    11881 tcctgtactt atatcaacat aagttagacc tatattagta ccatccttat acaaagataa
    11941 taaataatta ttctttttat tttcaagtaa atttccatct aaaactgtac caggagtgat
    12001 gactcttata acctctcttc taacaattcc ttttgctgta gatgggtctt ccatttgttc
    12061 acctattgct actttatatc cattttcaac taattttgat atatatgaat tggctgagtg
    12121 gaaaggaaca ccacacatag gagcacgttc ttctaaacca caagcttttc ctgtcaatgc
    12181 tatttcaaga gcttttgaag ctactaatgc atcttcaaaa aacatttcat aaaaatctcc
    12241 aagtctaaaa aacagtatac agtctttata tctatttttt acttcaagat actgtttcat
    12301 cataggagtt aatttattca tatctatttg cattatcaaa cctccttctt ttctaattaa
    12361 atattatatc atagtagatg attattatta acacaatata taaaattttg acatataact
    12421 aaaaacaatc caaaatgtgt actcacatt
//
LOCUS       DAEPXF010000108        12341 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain HB27
            SAMN13376389-rid12479423.denovo.114, whole genome shotgun sequence.
ACCESSION   DAEPXF010000108 DAEPXF010000000
VERSION     DAEPXF010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13376389
            Sequence Read Archive: SRR10518432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 12341)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12341)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001069624.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001069624.1
            Genome Coverage       :: 315x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/15/2021 01:54:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,165
            CDSs (total)                      :: 4,076
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 89
            rRNAs                             :: 10, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 10 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 19 of 69
            Pseudo Genes (incomplete)         :: 44 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 10 of 69
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12341
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13376389-rid12479423.denovo.114"
                     /strain="HB27"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="China: Hebei"
                     /collection_date="2017"
     gene            347..1036
                     /locus_tag="KRL67_002588"
     CDS             347..1036
                     /locus_tag="KRL67_002588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBH1523456.1"
                     /translation="MDILLVEDDKNIRQMVYDFLISENYSVKTAVDGNTAIEIFKNYS
                     FDIILLDLMLPKSSGFEVLKYIRSVSVVPVIITTAKDSDTDKMLGLNLGADDYITKPF
                     SIVELLARIKANIRRATVYSSSRSSQDIVIRYKDLIINISKYVVSKNGVDLNLTHTEF
                     EIVRLLATNQGRAFSKEQLYNTIWKEPYYGNENVLNTHINRLRNKLSDGTEKNNYIKT
                     LWGIGYKMEEE"
     gene            1036..1953
                     /locus_tag="KRL67_002589"
     CDS             1036..1953
                     /locus_tag="KRL67_002589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HBH1523457.1"
                     /translation="MELFLVVISIVLFSLYLKERNKSKKMESDYKYINSRLRDITNDN
                     ESNYILVPSDISIVKETAVDINKLLEKFYSRQIDYNRSEKAILQIFTNISHDLRTPIT
                     VLKGYIEMLYLQSQKEELSSSMRATVEKMQTNSNELVNSINNLFNMAKIQSGDMILNL
                     QKSNLTQLCHEIILEFYDLLDKENFNVEVNIEDRPIYANVDTEALQRILKNLIDNAIK
                     YGSTGRFLGISLYEKEKHIFIDIEDKGSGISETEKENIFTRAYTADRKKGNGLGLAIS
                     QGLANSMGGSIYVNSRPNIKTVFTVKLKS"
     gene            2026..2952
                     /locus_tag="KRL67_002590"
     CDS             2026..2952
                     /locus_tag="KRL67_002590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBH1523458.1"
                     /translation="MNYIIETHQLKKIYKDKAVVNDVNIHVKKGEIYGFVGPNGAGKS
                     TVMKMLLNLVKSNSGEIVMFGKKVAETDFEILKKIGTIIENPYFYENLTAKQNLDLHC
                     EYMGYYNKEHISEVLECVGLSKQSNKKVSKYSLGMKQRLAIARAILTKPELLILDEPI
                     NALDPEGIREMRELFRKLNTDYGITIFISSHILSEVEQIADTIGIIQDGKLIKEISMS
                     DIHKYQTDYVEVDVDNVELAGYLLEKEFGIKDFKITSESCIEIYDLRKDVKEISKIFI
                     KNQIGINSIGRKQSSLEDYFFQTTGTGGKETL"
     gene            2949..3629
                     /locus_tag="KRL67_002591"
     CDS             2949..3629
                     /locus_tag="KRL67_002591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBH1523459.1"
                     /translation="MIHLMKLELKKVGLKRYFLFSMLGILLCMFFVFTGLNDKATSIY
                     DYDVTFRTISLVFCFYYIILFAVLNVAYIINEYTNKTILILFSYPLDRKKLILAKLLL
                     ITTLMISSMIVGYICCFTFVVVLDKYFNLVIGDFHISILSYWIPGAIKSIIVFCSLGI
                     WAFVVGMMRKSIPVTLVSSIIFIHLRQFILAGTNTTQDSLISVFIIVGITVIGIYYIL
                     NHKVAEID"
     gene            4073..4774
                     /locus_tag="KRL67_002592"
     CDS             4073..4774
                     /locus_tag="KRL67_002592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HBH1523460.1"
                     /translation="MNNSFLDKLIETRELKNSLYNVLKHNFLYHSNKIEGSTFTTEAL
                     ALLLDKNVVTGRHTLDDVQETVNSSHVFDTIIDSLKEKITHNFLRNLHSSLIFNTTLH
                     FRGMSGIYKTIPNMILGTDVSIAQPFEVEPKLDELIEWYYSQSEVSIKVIAEFHYRFE
                     LIHPFQDGNGRIGRFVMLKQMLENNLPIKIVSWDSEDLYRNSLNSCSLGNYVPLIEYL
                     SSLEDFREVYKMLWK"
     gene            5518..6168
                     /locus_tag="KRL67_002593"
     CDS             5518..6168
                     /locus_tag="KRL67_002593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBH1523461.1"
                     /translation="MKVKSNLDEILYHKIIESLIRGEYSVGQKILLNDLCEKFEVSRT
                     PVVQAVKMLNKDGVLTIMTNGKVYVPEYEYDMVKQVCETRTLIETYALEKMMQEEEDV
                     FQQKLDVIKRYSDKCEEYYRQEKSVELALADLELHKAIVEGANNEILNDVYVGIQGRF
                     IVVNYLIRPLKNRNYEGTVQDHFEILKFIEDRDTKKAVDKLRNHIQGTIKRFCEDE"
     gene            6470..8536
                     /locus_tag="KRL67_002594"
     CDS             6470..8536
                     /locus_tag="KRL67_002594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HBH1523462.1"
                     /translation="MKEVVWSLKKITKERIENTMKKLFTSETFLEYQFISDCQISPDG
                     GYTAFIVKKADIKENAYTSQVYILNNKSGELKQITSINSVGAYAWKDENTILFPALRN
                     EKVKEAVKNGKQCMSYYALSLNGGEAEELFRLPIKGGKLNPIGKGLYAVIDSYDNDRP
                     IVEGLSEEEQQKKINAYNKRHYEHFKEIPYAVNGEGYISRKRKRLYLYDSNINELKAI
                     TAPMFNVVGMKISDGKILYIGQEFKDVKGLKNGVYVFDTNTNTNVCILEKDKYIIKGF
                     ELYQNQVILNLTDALSYGNGENGDFYTIDIDTKEMKLLSEHQHHCIGNTVTSDVKMGA
                     GQTTKVDGEYIYYTSTVDMDCIIERIHIPTGKQEKVTQTGSVDFIDVKDGNIVCVGCL
                     GNGLPEVYTVENGTLCKKTHLNDHILEEYKISVPEYIESKGSSKWEIQGYVYKPVDYE
                     VGKKYPAVLAIHGGPRLTYGPYFMHEIQVFASAGYFVFFCNPRGSEGRGNAFADIRKQ
                     FGDIDYIDFMEFTDTVLEKYTDIDKTKLAVEGGSYGGFMTNWIIGHTNRFAVACAQRS
                     IANWSGMEGTTDIGYYFCKGQTGASHMENHELQWKQSPLAYADKCVTPTLFLHGEKDY
                     RCYMQEAFQMFSALKIHGCPTKLCLFEGENHELSRSGRPKQKLQRLVEMLDWFSVYIK
                     KDNIGQ"
     gene            8555..10510
                     /locus_tag="KRL67_002595"
     CDS             8555..10510
                     /locus_tag="KRL67_002595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HBH1523463.1"
                     /translation="MEKIKIENSDIFEYIFPHDISCSPDGKHIAYIISNINEEKDCYE
                     HDLYVMDIKTEKQIHMTQTKDVTSFSWVSNTELLFTSKRNKPKAGTTDFYTISIEGGE
                     AKKAFSIPKACSVPISLGNKLWLLTTKNPTDSKKSEPDRAVEGIDYWTFTDKPFIRDG
                     ENFSQRRRVTLELYQEGENTTKAITPKFCEVASIDVSSDKKRILYTGQIYEDCATPFS
                     GLWEYHIDSGETKELVPQGKYQISLAKYIGKDKVMLQASTLDRSITQNHDIFILDLSS
                     SEMNMIASPDGMYATLLDVDAVYGGGRSNKVIGDKFIGARICRTMTEFNEFDTKTGNI
                     RIITKVDAFTSFDIYDNTMYTVMLKDYELAEIYSIDMTTGTMKKMTSFSKPYLDTHKV
                     SLPEKLTFVAKNKEEVDGFVIPPIDAKEGEKYPAVLFIHGGPKWAYGYMFTHLKQCVT
                     SKGMYVIYCNPHGGDGYGEKFLEMVERWGYVDYEHLMEFVDTCIEKYPGIDADRLGVA
                     GGSYGGYMTNWIIGHTDRFKAAASQRGISNLITASLIIDFGDRIMKQTCGDKTPWNHE
                     EVLWNHSPIKYVKNVKTPTLFLHSDRDYRCFMGDTFQMFTALKQIGVDTEMYLFHGDT
                     HGLSRNGRPSNRIARANAIVDWFERYL"
     gene            10526..11716
                     /locus_tag="KRL67_002596"
     CDS             10526..11716
                     /locus_tag="KRL67_002596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HBH1523464.1"
                     /translation="MSDMLLWFSVVLVLFAIGDLIASKTKAKVSAVFVTLLLFLILFV
                     TKAIPADIIEKAGMTAAASWSVPMIMFSMGTMLNVKQFIDEWRTVLTAWLGIVAVIVC
                     VSLCIPLFGKSTVLTSIPVINGALPATTIMTQAALEKGLTLAAATATVVFAIQKFIGT
                     PIASRAALQEANRLLVEYHEAKSKGIDLANVDTDKKEAENTGTKVKQAFCEKYDKYYS
                     TNVCIFFIALFSYLGYELSEIIHVNYSIVCLVVGVLVTRIGIVPKDILEKGKIKGFIN
                     MVVFAAVIPSLAKVSLTDLISLFVPIVGMFAASIIGIFLMMKVLPGWKIIGSKPLAFG
                     VGFCQMLGFPTTYLISNEVCNAVGETEEERAYLMSKIMPKLVVGGMACMISIVVAGLM
                     VPML"
BASE COUNT         4668 a         1394 c         2165 g         4114 t
ORIGIN      
        1 aagataatat gcaaattatg atgtctaaat tccttagaat cttgacaata aatttgagat
       61 aatagataaa gaattttttt gaaatttaat gattcagagt aataatgcta atagtatttt
      121 gtatctaatt taatcattca catctatggg atttacatat tttagataaa gataagagag
      181 tatactatat tttaattatt ttttcagtaa agcttattga aaagaacttt aacttagaag
      241 agtatcgcca aattgcagat acaatatcat aagcagttat ttattgacaa ctctttttaa
      301 taaatatatc atttgaagta tattgacaaa aaggagttga ataataatgg acatcttatt
      361 ggttgaagat gataagaata ttcgacaaat ggtatacgat tttctgatta gtgagaatta
      421 ctctgttaaa actgccgttg acggaaatac agctattgaa atatttaaaa attattcatt
      481 tgatattatc ttactggact tgatgttacc aaagtctagt ggttttgaag tattaaagta
      541 tatacgaagt gtgagtgttg ttcctgttat tattactact gcgaaagata gtgacacaga
      601 taaaatgcta gggcttaatc ttggtgcaga tgattatatt acaaagcctt tttcaatcgt
      661 agaattgctt gcaagaatta aagctaatat tagacgtgca acagtgtata gcagttctag
      721 gagttctcaa gatattgtta ttcggtataa agatttgatt ataaacatat caaaatatgt
      781 agtttcaaag aatggagtgg acttaaactt aacacataca gaatttgaaa ttgttagatt
      841 actagctacc aatcaaggac gtgctttttc aaaggaacaa ttatacaaca ctatttggaa
      901 agagccttat tatggaaacg aaaatgtgct aaatactcat attaacaggt taagaaataa
      961 actgtctgat ggtacggaaa aaaataatta tattaaaact ctttggggaa ttggctacaa
     1021 aatggaggaa gaataatgga gttattttta gttgtaattt cgattgtact attttctctc
     1081 tacttaaaag aacgaaataa gtcaaagaaa atggaaagtg attataagta tatcaattca
     1141 agacttcgtg acataacaaa tgataatgaa agtaattata ttttagtgcc atctgatatt
     1201 tcgattgtaa aagaaacagc agtagatata aataaacttc ttgaaaagtt ttacagcagg
     1261 caaattgatt ataatagaag tgaaaaagcg atattgcaaa tatttacaaa catatcacac
     1321 gatttacgaa ctcccatcac agtattaaag ggttatattg agatgttata tcttcaaagt
     1381 caaaaagaag aactatcttc ttcaatgaga gcaactgtag aaaaaatgca aacaaattca
     1441 aatgagcttg ttaattcaat aaataatctg tttaatatgg caaaaatcca atctggagat
     1501 atgatattaa atcttcaaaa atccaatcta acacaattat gtcatgagat tattttagag
     1561 ttttatgact tgctagataa agaaaacttt aatgttgaag ttaatattga agatagacct
     1621 atatatgcaa atgttgatac agaagcatta caacgtatac tcaaaaattt aattgataat
     1681 gctataaaat atggttcaac tggtagattt ttagggattt cactatatga aaaggaaaaa
     1741 catattttta tagatattga ggataaaggt tcaggtattt ctgaaactga aaaagagaat
     1801 attttcacga gagcctatac tgctgacaga aagaaaggca atggtttagg actagctatt
     1861 tctcaagggc ttgcaaactc tatgggaggt tcaatttatg taaatagtag acctaatata
     1921 aaaacagttt ttacagtaaa attgaaaagt tagaaaaaag ttagatttaa aagagagaaa
     1981 agataatttt attttgtata ctgtttttag aaaggaggga gtatgatgaa ctatatcata
     2041 gagacccatc aattaaagaa aatatataaa gataaagctg ttgttaatga tgttaatatt
     2101 catgtgaaaa agggagaaat ttacggattt gttggaccaa acggagcagg taaaagtaca
     2161 gtaatgaaaa tgttgctaaa tctggttaag tcaaattctg gagaaatagt tatgttcggt
     2221 aagaaagtgg cagaaactga ttttgaaata ttaaaaaaga ttggtactat tattgaaaat
     2281 ccttatttct atgagaattt gactgctaaa cagaatcttg acttacattg tgaatatatg
     2341 ggttattata acaaagaaca tatatcagag gtattggaat gtgtcggatt gtcaaagcaa
     2401 tcaaacaaga aagtttcaaa atattcttta ggtatgaaac aacgtcttgc aatagctaga
     2461 gcaattctta ctaaaccaga attattgatt cttgatgaac ctataaatgc actcgaccca
     2521 gaggggataa gagaaatgcg agaattattc cggaagttaa atacagatta tggaataact
     2581 atatttattt ctagtcatat tctttcagag gtagaacaaa ttgcagacac tattggtatt
     2641 attcaagatg gtaaacttat taaagaaatt tctatgtcag atatacacaa atatcaaaca
     2701 gattatgttg aagtagatgt agataatgtg gagcttgctg gatacttgtt agaaaaagaa
     2761 tttggaataa aagactttaa aattacttcg gaaagttgta ttgagatata tgatttacga
     2821 aaagatgtaa aagaaatatc caaaatattt atcaaaaatc aaattgggat aaatagcatt
     2881 ggacgtaaac aaagttcttt agaagattac ttctttcaaa ctacaggtac aggaggtaaa
     2941 gaaacactat gatacattta atgaaactag aattaaagaa agttgggcta aaaagatatt
     3001 ttttattttc aatgctgggg attttgttat gtatgttttt tgtctttact ggattgaatg
     3061 ataaagctac ttcaatatat gattatgatg ttacatttag gacaatcagt ttagttttct
     3121 gtttttacta tattatatta tttgctgtgt tgaatgtggc atatataata aatgaatata
     3181 caaataaaac aatcttgatt ttattctctt atccacttga ccgtaaaaaa cttattttag
     3241 caaagttact tttgattacg actcttatga tatcatcaat gatagttgga tatatctgtt
     3301 gttttacatt cgttgttgtg ttggataagt attttaattt agttattgga gattttcata
     3361 tttcaatttt atcttattgg ataccaggag caattaaaag tattattgta ttctgctcat
     3421 taggtatatg ggcattcgtt gttgggatga tgagaaaatc aatacctgtt acccttgtca
     3481 gttcaattat ttttatccat ttaagacaat ttattttagc tggtacaaat acaactcaag
     3541 acagtttaat ttctgttttt attatagttg gtataactgt gattggtata tattatatac
     3601 taaatcataa agttgctgaa attgactaga tactattgat aggaaggtag ttattaattg
     3661 tatcttcttg ggataataat gatttgctat tatatcagct attattaata ttattagagt
     3721 tttaatagta ttaataatag ctatatattt tttcttctta tttataaatt taatgattta
     3781 tagtcataaa atgaactcta agaagagata aaaatatgtt ttatatgttt ttagattaat
     3841 ataaaatatc acaatgaaat gttgtacaaa gtgatacata ataattatat gaactataaa
     3901 attgactaaa cttattgact aataaattag atgaaaagtt gagacttact gaaaataaat
     3961 acaatgaaat atatactaca agtattaaat taaatctgaa ctttacatac tattagaata
     4021 agtattataa gattataatg atttaaaaat attaaaataa ttgaggtgat acatgaacaa
     4081 ttccttttta gataaattga ttgagacaag agaacttaaa aattcacttt ataatgtgtt
     4141 aaaacacaat tttctttatc attctaataa gatagagggc agtactttta ctactgaagc
     4201 attagcactt ttacttgata aaaatgttgt tacaggaaga catactttag atgatgtaca
     4261 agaaactgtt aattctagcc atgtgtttga tacaataatt gactcactga aagagaaaat
     4321 aacacataat ttcttaagga atctacattc aagtttaatc ttcaatacaa ccctacattt
     4381 cagggggatg tctggtatat ataaaactat accaaatatg atactaggaa ctgatgtaag
     4441 catagcacaa ccatttgaag ttgagccaaa gcttgatgaa ttaattgaat ggtactatag
     4501 tcagagtgaa gtatctatta aagttatagc tgaatttcat tatagatttg aattgataca
     4561 tccatttcaa gatggtaatg gtcgcatagg tagatttgtt atgctaaaac agatgttaga
     4621 aaataatctt cctataaaaa ttgtatcttg ggatagtgag gacttgtata gaaactcctt
     4681 aaattcttgt agtttaggta attatgtccc tttaattgaa tatttatcaa gtttagaaga
     4741 ttttagagaa gtatataaaa tgttatggaa ataattgttt gtagcaaaaa aagtttttat
     4801 ataaatatat ttttaactat gctatttaac gttgatttct tagttattct tagcttggac
     4861 tatacccact attttaacta tataagatag tggatatagt ttaactatct ctttataact
     4921 atttttatgt atcttaattg tttcttaata tggggacagt tcataaaact aaaagtgatt
     4981 tttaatttta tcatttataa tttgacatta catcagaaaa atgcttatca ttagaatttt
     5041 cataaaaggt acctattttt ttcaattaac taaatcaatt ctattagtct taatctttcg
     5101 tatttcaaat tgataataca agaaaataaa aaataaaaaa ttttaaattt tttatttatt
     5161 gcgaaaaaaa taaaaaggtg ctatactttt tgtaacatat tattcaaatt attgagagat
     5221 agtaaacatt tatagtttaa tttgtagtac aatagcatga tttgaagtat aacgatttaa
     5281 tctatagtat aatagtttaa tttgaagtac aacgatttaa tctatagtac aataacttga
     5341 tttgcaatac aatgatttaa tctatagtat aatagcatga tttacagtac aataatttaa
     5401 attgtagtac aaaagtacta tcaaataaga atttctactt taaaaatatt actaaaagta
     5461 attaaagtcg agtaattcaa tataatatgg caaaagaata gaaataaggt gagagtagtg
     5521 aaagtaaagt caaatttaga tgaaatttta tatcataaga ttattgaaag tcttatccgt
     5581 ggtgaatact ctgtaggtca aaaaatatta ctaaatgacc tttgtgaaaa atttgaagta
     5641 agcagaacac cagttgtaca agcagtaaaa atgctaaata aagatggtgt acttactatt
     5701 atgacaaatg gtaaagtgta cgtaccagag tatgaatatg atatggtaaa acaagtttgt
     5761 gaaacgagaa cactgattga aacatatgct ttagaaaaaa tgatgcaaga agaggaagac
     5821 gtttttcaac aaaagttaga tgtaataaaa agatactctg ataaatgtga ggagtactat
     5881 cgtcaagaaa aatctgtaga acttgcacta gcagatttgg aattacataa agccattgta
     5941 gaaggggcaa ataatgagat tttaaatgat gtatatgttg gtatacaagg aagatttata
     6001 gtagtgaact atttaattcg accattaaaa aatcgtaatt atgaaggaac agtacaagac
     6061 cattttgaaa tattaaaatt cattgaagac agagatacaa aaaaagcagt agataaatta
     6121 agaaatcata ttcaaggaac tattaaaaga ttctgtgaag atgaataata agttttgaag
     6181 ttgctatata tacttatcta ttaagttgct aaatgtagtc taagtataat ctaattatag
     6241 tttaaacata atctaaatat agtgtaaata agtctaagta taaattaaat atagattaag
     6301 tatagactaa gtatggatta agtataaatt aagtaccaat taagcatgga ttaaatatac
     6361 tttaaattac aaatattaca aggaagccag aaggcttcct aaaaaaataa cctttataaa
     6421 attttttatt ttaaatttaa aattttttat tttaaattta aaataaaaaa tgaaagaggt
     6481 ggtatggagt ttgaaaaaaa ttactaaaga aaggatagag aatactatga aaaagctatt
     6541 tacgagtgaa acatttttgg aatatcaatt tataagtgat tgccagatat caccagatgg
     6601 aggatataca gcatttattg taaaaaaagc tgatatcaaa gagaatgctt acacaagcca
     6661 agtttatata ctaaacaata aaagtggcga attaaaacaa ataacatcta taaactctgt
     6721 tggagcatat gcctggaaag atgaaaatac tatcttgttc cctgctttgc gaaatgagaa
     6781 agtaaaagaa gctgtcaaaa atggtaaaca atgtatgagt tattatgctt tatctttaaa
     6841 tggtggagaa gcagaagaac tttttagact ccctatcaaa ggtggtaaat tgaaccctat
     6901 aggaaaaggt ttgtatgcag ttattgattc ttatgacaat gaccgaccta ttgtagaggg
     6961 attgtcagaa gaagaacaac agaaaaaaat aaatgcttat aacaaaagac actatgaaca
     7021 tttcaaagaa attccttatg ctgtcaatgg ggagggatat attagcagaa aaagaaaacg
     7081 cttatatcta tacgacagta atataaatga gttgaaggca attacagcac ctatgtttaa
     7141 tgttgtaggt atgaaaataa gtgatggaaa aatcttatat atagggcaag agtttaaaga
     7201 tgtaaaaggt ttgaaaaatg gggtctatgt atttgataca aacacaaaca caaatgtttg
     7261 tatattagaa aaagataagt atattataaa aggttttgaa ttatatcaaa atcaagtgat
     7321 actaaaccta actgatgctt tatcttatgg aaatggagaa aatggagatt tttatactat
     7381 tgatattgat actaaagaaa tgaaacttct ttcagaacat caacatcatt gtattggaaa
     7441 tacagtaaca tctgatgtta aaatgggtgc tggtcagaca acaaaggtag atggagaata
     7501 tatttattac acttctacag tagatatgga ttgtattatt gaaagaatac atattccaac
     7561 aggaaaacag gaaaaagtaa cacagacagg ttcagttgat tttatagatg taaaagatgg
     7621 caatattgta tgtgtgggtt gtttaggaaa tggattacca gaagtttata cagtagagaa
     7681 cggaacattg tgcaagaaaa cacatttaaa tgaccatatt ttagaggaat ataaaatttc
     7741 tgtgccagaa tacatagagt caaaaggaag ttctaaatgg gaaatacaag gatacgtata
     7801 taaaccagta gattatgagg tggggaaaaa atatccagct gtattggcga ttcatggagg
     7861 accgagattg acatatggac catattttat gcatgagata caggtatttg caagtgcagg
     7921 ttactttgta ttcttctgta acccaagagg gtctgaaggt cgtggcaatg cttttgcaga
     7981 tattaggaaa cagtttggag atattgatta tatagatttt atggaattta cagatactgt
     8041 acttgaaaaa tatacagata ttgataaaac aaaattggct gttgaaggtg gaagttatgg
     8101 tggatttatg acgaattgga taattggtca tacaaaccgt tttgctgttg cttgtgctca
     8161 gcgttctatt gcaaactgga gtggcatgga gggtacaaca gacattggtt attatttctg
     8221 caaaggacaa acaggtgctt ctcatatgga aaaccacgag cttcaatgga aacagtcacc
     8281 tcttgcttat gctgataaat gtgttacacc gactttattt ttacatggtg aaaaagacta
     8341 tagatgctat atgcaagaag cattccagat gttttcagca ttgaaaattc atgggtgtcc
     8401 tacaaaactt tgtctatttg aaggggaaaa ccatgaacta tctcgttctg gtagaccaaa
     8461 acaaaagcta caaaggttag ttgaaatgct agattggttt tctgtgtaca taaaaaaaga
     8521 taacatagga cagtagagaa aggagaatat aaaaatggaa aaaataaaga ttgaaaattc
     8581 agatattttt gaatatatat tccctcatga tatttcttgt tcacctgatg gaaagcatat
     8641 tgcttatatt atatcaaata tcaacgagga aaaagattgc tatgaacatg acttatatgt
     8701 gatggacatc aaaacagaaa aacaaataca tatgactcaa acaaaagatg taacatcatt
     8761 tagttgggtt tctaatacag aactactttt tacatctaaa agaaataagc caaaagcagg
     8821 tacaacagat ttttacacta tttctataga aggaggagaa gcaaaaaaag cattctctat
     8881 cccaaaagct tgtagtgtac ctatatcact tggaaataaa ttgtggttac tgacaacaaa
     8941 aaatccaacg gattctaaaa aatcagaacc agatagggca gtagagggta tagattattg
     9001 gacatttaca gataaacctt ttataagaga tggagaaaac ttttctcaga gaagacgtgt
     9061 aacattagaa ttatatcaag aaggtgaaaa tacaacaaaa gccattacac caaaattttg
     9121 tgaagtagca agtatagacg tttcttctga taaaaaaaga attttatata ctggtcagat
     9181 ttatgaagat tgtgcaactc ctttttctgg actttgggag tatcatatag acagtggtga
     9241 aaccaaagaa cttgtaccac aaggaaagta ccaaataagt ttggcaaaat atattggcaa
     9301 agataaagta atgttgcaag cttcaacatt agaccgttct atcacacaga atcatgatat
     9361 ttttatactt gatttgagtt ctagtgaaat gaatatgatt gctagtccag atggtatgta
     9421 tgctacacta ctggatgtag atgcagtata tggtggtgga cgtagtaata aagttatagg
     9481 agataaattt attggtgcaa gaatttgcag aactatgaca gaatttaatg aatttgatac
     9541 aaagacaggt aatatacgta tcatcacaaa agtagatgct tttacatctt ttgatattta
     9601 cgacaacacc atgtacacag taatgttgaa agattatgag ttagcagaaa tatatagcat
     9661 tgatatgact acaggtacaa tgaaaaaaat gacttctttt tctaaaccat atttagatac
     9721 tcataaggtt tcattacctg aaaaattgac gtttgtagca aaaaataaag aagaagttga
     9781 tggttttgtt ataccaccga tagatgctaa agaaggcgaa aaatatcctg ctgtattgtt
     9841 cattcatggt ggacctaaat gggcatatgg atatatgttt acacacttga agcagtgtgt
     9901 aacatcaaag ggaatgtatg tgatttattg taaccctcat ggtggtgacg gttatggtga
     9961 aaaattctta gaaatggtag aaagatgggg ttatgttgac tatgagcatc ttatggaatt
    10021 tgtagatact tgtattgaaa aatatcctgg gattgatgca gaccgcttag gggttgcagg
    10081 gggaagttac ggtggttata tgacaaattg gataattggt catacagacc gtttcaaagc
    10141 agcagcttct caaagaggta ttagtaactt gattacagct tctttaatta ttgattttgg
    10201 ggatagaatt atgaagcaga cttgtggaga taagacacct tggaatcatg aggaggttct
    10261 ttggaatcat tcacctatta aatatgtgaa aaatgttaag acaccaacat tgttcttaca
    10321 tagtgatagg gactatagat gttttatggg ggatactttc caaatgttta cagcattaaa
    10381 acagattggt gtagatacag aaatgtattt gttccacgga gatacacatg gattgtctag
    10441 aaatggaaga ccatctaata gaattgcacg tgcaaatgct attgtagatt ggtttgaaag
    10501 atatttataa taagggggtt attctatgag tgatatgtta ttatggttta gtgtcgtatt
    10561 agttttattt gctattgggg atttaattgc aagtaaaact aaagcaaaag tatcagctgt
    10621 atttgttaca ctacttttat ttttgattct atttgtcaca aaagcaatac cagctgatat
    10681 tattgaaaaa gcaggtatga ccgcagcagc tagttggagt gttccaatga ttatgtttag
    10741 catgggaaca atgttgaatg ttaaacaatt tatagacgaa tggagaacag tattgacagc
    10801 atggcttgga attgtagcag ttatcgtttg tgtctcatta tgtattcctt tgtttggaaa
    10861 atcaacagta ttgacaagca taccagttat aaatggagct ttacctgcta ctactattat
    10921 gacacaagca gcactagaaa aaggactgac acttgcagca gctacagcaa cagttgtatt
    10981 tgcaattcag aagtttattg gtacaccaat agcttcaaga gcagcactac aagaggcaaa
    11041 tcgtctttta gtagaatacc atgaagcaaa atcaaaaggc attgatttag caaatgttga
    11101 tactgataag aaagaagcag agaatacagg cacaaaagta aaacaagctt tctgtgaaaa
    11161 atacgataaa tattattcta caaatgtttg tatatttttt atagcactat tctcttatct
    11221 agggtatgaa ctttcagaga ttattcatgt aaattattct attgtatgtc ttgttgttgg
    11281 tgttcttgta acacgtattg gtattgtgcc aaaagacatt ttggaaaaag gaaaaatcaa
    11341 gggatttatc aacatggttg tgtttgcagc tgttattcct tctttagcaa aagtttcttt
    11401 gacagattta attagtcttt ttgtaccaat tgtaggtatg tttgcagctt ctatcattgg
    11461 tatattcctt atgatgaagg tgttaccagg ttggaaaatt atcggttcta aacctcttgc
    11521 ttttggagta gggttttgtc agatgttggg attcccaact acatatttaa tttcaaatga
    11581 agtgtgtaat gctgttggag aaacggaaga agaaagagca tatttgatgt ctaaaattat
    11641 gcctaagctg gtagttggtg gcatggcttg tatgatttct attgtagtag ctggtcttat
    11701 ggtaccaatg ttatagatag atttcatagt taaaaaagtg ctattaattt ataggagaag
    11761 ctaaaaagca gtattaattt ataaataaag ctagaaagta aaattaattc ataaacgaat
    11821 tttatattag ttaatttgtt ataagaatat tttcttttta gcattataat cttgatttta
    11881 tcaaggaaca gattttttat ttgctattct aatagttaca gtttattttt attttctaaa
    11941 atacaacttt aacttgattt atgtttaaat agggtattta ataatatttt tggaaaatct
    12001 ttagaaagaa ttaaaaagag aagaaaaaat ggagtgcctc aaagtgaatt tcttagttca
    12061 taaggcactc ttttttgtat gaaatcgaat atattttaat cattttataa gagatggaga
    12121 aaatttttct caaagacaat gtttaacatt agacttgtat caagaaggtg aaaatacaat
    12181 tcaatagtga gaaagagatt tatatctagt ttgagacaag tctttttaat tattaaagtt
    12241 attttttatc ttatttaaaa aagagcatca gtattttata aattagtatt ttttataagt
    12301 agtttatcac ttaatttatc attaaacaaa tcatacagga t
//
LOCUS       DAEPXF010000109        12163 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain HB27
            SAMN13376389-rid12479423.denovo.115, whole genome shotgun sequence.
ACCESSION   DAEPXF010000109 DAEPXF010000000
VERSION     DAEPXF010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13376389
            Sequence Read Archive: SRR10518432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 12163)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12163)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001069624.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001069624.1
            Genome Coverage       :: 315x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/15/2021 01:54:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,165
            CDSs (total)                      :: 4,076
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 89
            rRNAs                             :: 10, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 10 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 19 of 69
            Pseudo Genes (incomplete)         :: 44 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 10 of 69
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12163
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13376389-rid12479423.denovo.115"
                     /strain="HB27"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="China: Hebei"
                     /collection_date="2017"
     gene            133..1014
                     /locus_tag="KRL67_002597"
     CDS             133..1014
                     /locus_tag="KRL67_002597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase"
                     /protein_id="HBH1523465.1"
                     /translation="MVKKRKKKTVTLTILVVIVIGIFSMAWADMSRKKEMKETQERLK
                     AERLEKERIEKEKNEPIIGAKKEAVKEYGYDAKIVWDKLNKYDYSNNGEKIVFLTFDD
                     GPSTTNTPQVLDILKRHGVRGTFFIKGDSLERKGANEILKRTFDEGNAIAHHSYTHDY
                     KKLYPSRSLNLDTFVNELNKTDEAMKKALGKNFSSNVVRCPGGYMSWKNMEPLGNYLK
                     EKNMASIDWNALNADAEGKKKNAQELFEHAKKSSEGKEMVVLLMHDTYGKQETVNALD
                     QIITYFKDNGYQFKILI"
     gene            complement(1075..2283)
                     /locus_tag="KRL67_002598"
     CDS             complement(1075..2283)
                     /locus_tag="KRL67_002598"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HBH1523466.1"
                     /translation="MKSIDEQMRIIMKGVDDLIDEKELREKLIKSEKEGKPMIVKLGL
                     DPSAPDIHLGHTVVLRKMKQLQDLGHQIVIIIGDFTGKIGDPTGKSKARKALTTEQVL
                     ANAKTYEEQIFKVLDKEKTIVRFNSEWLAKLNFEDVIKLAATITVARMLEREDFKKRY
                     EGQMPISVHEFFYPLMQAYDSIALEADIELGGTDQRFNLLMGRSLQREFGMESQIVIM
                     MPLIEGLDGKEKMSKSLGNYIGIDEEAGIMYQKSMEIPDELIIKYYNLVTDVHPDEVN
                     KIESQLKEGSVNPRDIKMNLAREIVTLYHGEESAREAEERFKSVFQKGQIPEDIQTIQ
                     VEEDGFDLIEVLVSNEIVKSKSEVRRLASQGGVKVNGEKVEDLSTIVKESELVVQIGK
                     KKFVKIELVK"
     misc_binding    complement(2356..2608)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(2760..3749)
                     /locus_tag="KRL67_002599"
     CDS             complement(2760..3749)
                     /locus_tag="KRL67_002599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2232 domain-containing protein"
                     /protein_id="HBH1523467.1"
                     /translation="MIYGGSVLNTTKKLTEAALLSSLFIVVTIIAVSTGFGYAIYLDF
                     IVPVFFCIICLKCDLKYTILSGITSLLIISLVLGNLGTAIWASQSVLLGIMCGYLINK
                     PTMVMDDLVYGSVFGVIVMVFIDIYASTLIGYSFMKEFQGYSKWIYFNGYTNFIYYLM
                     IALFPFGMVFCIYLLSLILGNKLHILDSNSLKKFLIFKNFRSLNQFLCCSKKAFYICV
                     SYLAIFEVLKLWNVKVDSVYLKTIFISITYISVYFIIRDSCMSLQNFIIAKFRKLLYA
                     RILFLIIILLLFFIFDVTVISLIIINAFLNKKIDIRLSQSNIVNKQINLLIEK"
     gene            3901..4341
                     /locus_tag="KRL67_002600"
     CDS             3901..4341
                     /locus_tag="KRL67_002600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBH1523468.1"
                     /translation="MFKIKHFNDLSLDEFYEIAKSRYEVFACEQKIFSLNDYDDIDKS
                     SYHIFLKENGLICAYARIIPKEYSSYNDVSIGRVLVLSSHRRKGLAKQMMDCAIDFIK
                     VNLHENNITLSAQTYIKNLYLSCGFKEISEVYDEAGIEHIKMRL"
     gene            4668..4895
                     /locus_tag="KRL67_002601"
     CDS             4668..4895
                     /locus_tag="KRL67_002601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HBH1523469.1"
                     /translation="MKNLNDIQVKKTVKVEDILSSGNLRERMLALGLTRGAVIDVVRK
                     GPKNNLTVYKIRGSKIALRQEESSLILVSDI"
     gene            4921..7041
                     /gene="feoB"
                     /locus_tag="KRL67_002602"
     CDS             4921..7041
                     /gene="feoB"
                     /locus_tag="KRL67_002602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HBH1523470.1"
                     /translation="MGLTHNSTKMSSLKDMFDIDNKEDQFIIALAGNPNTGKSTVFNH
                     LTGLRQHTGNWPGKTVATARGNFKYKNTEYALIDLPGTYSLFALSQEEIVARDFICFG
                     NPDAVIVVCDATCLERNLNLVFQVMELTDKVILCINLIDEARKKGITIDKKLLEDSLG
                     IPVILTAARNGSGMDELLDTLNDVSFDKYKLNNKPVRYNENIEDVVKSIQPELDNIIP
                     GINSRWLGLRLIDGDESIFESMSNYIDKDSIDAINEVKKKIPDNINKQKIRDEFTKIN
                     YDYAKKLSDECCSNVAKKSTDREEKVDKILTSKIFGLPIMLLLLGTILWITIEGANYP
                     STLLSNLLLGFEPSISGILNSINCPSWLNDMLVLGLYRTLAWVISVMLPPMAIFFPLF
                     TLLEDFGYLPRVAFNLDHLFKKACAHGKQCLTMCMGFGCNAAGVIGCRIIDSPRERLI
                     AILTNNFVPCNGRFPTLIAISTIFFSSVITNSFVSSVATALCITLLIILGVIITLLVS
                     YTLSKTLLKGVPSTFTLELPPYRVPQIGRTLYTSIIDRTIFVLGRAVMVAIPAGVITW
                     IFANIYIGDLSILSHVANFLDPLAKLIGLDGFILLAFILGFPANEIVVPILLMAYLAT
                     GSMIELDSFSALGQVLREHGWTYLTALNVMLFSLLHWPCATTLLTIKKETGSLKWTAL
                     GFLMPTILAFVVCFLTTTVYNLFI"
     gene            complement(7174..7992)
                     /locus_tag="KRL67_002603"
     CDS             complement(7174..7992)
                     /locus_tag="KRL67_002603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HBH1523471.1"
                     /translation="MEKQYFTTGEFAKLCGISKQTLIFYDKIGIFSPEYKDKNNYRYY
                     SVYQYDTLDILQSLREIGMSLEEIKEYIQNRTPQLCVKMLKEEEKKIREKIKKLRKIS
                     SKMQNRISITVEGISKMNSEEIYISKKFEEYLVLSSDLSNMSNSDFMMELIDFTNYCK
                     SKNLYQGYELGVIVSNENIKNGDYLSISSFYLKIDKKIKDKKLYVKPEGMYACIKHIG
                     KYEDSYKSYEKLKKYIYENEYKIIGNSYEESILDFFCESNEDNYMTEISIQVSR"
     gene            8199..8483
                     /locus_tag="KRL67_002604"
                     /pseudo
     CDS             8199..8483
                     /locus_tag="KRL67_002604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889354.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     repeat_region   8641..9327
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=8773..8801
                     /rpt_unit_seq="atttacattccatatagttaatataaaac"
     gene            complement(9988..12081)
                     /locus_tag="KRL67_002605"
     CDS             complement(9988..12081)
                     /locus_tag="KRL67_002605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HBH1523472.1"
                     /translation="MNKRNFEVILNQLQINIYVTNIHTNEIIFMNKKMKEEYNILDPE
                     GKVCWQVLYPEQNSTCSFCKVLELLKNDKKGVLIKWYEKCNKLNRAFENYDSLITWQD
                     GTVVHMHQSIDIANSTILNKAIKINEFNEISNTKEEKGVFNFSRDNFDYNSTLLYDAL
                     IRGTDEYIYICNMKTGVFRYSPSQVELFDLPGEIVENPLIYWEKIVHPEDWNRFYKSN
                     TEIGKNQMDYHTVEFRAKNRSGEYIWLRCRGQLMRDEFGEPSIFAGIMTQLGKQNKID
                     SLTQLLNYHEFMSVFEDKISNPMIEKLCIVLLNIDDFKNVNEMYDRDFGDNIIKTLAQ
                     SIQSILPDNAELYKLDGDEMGILVDNVEENEILTLYDQIQNMIIHLQLWRKYGLNITI
                     SAGCVIYPKHGDTVKELYKCASYSLQYAKEHGKNRLVFFSQEILKNKMYSLEMMRDLK
                     ASINDDFRGFSLRFQPQVDTQSHKMIGVEVLLRWTNDKYKAISPLEFIPILEENDMIN
                     IVGAWVLRMALRTFRKWIDNYPFFKVSVNVSAVQILEDTFIKDIVKIIDDENFPYQNL
                     VLELTESHTVQNLSILQSKFKALQDLGIYIAMDDFGTGYSSLEVLKFSPIDIVKIDRV
                     FVKDILKSKFDATFIHFIVAICHDVGIKVCLEGVETQEEYDLVKQIKPDYIQGYLFGK
                     PQTATEIFDLLKLDN"
BASE COUNT         4318 a         1780 c         1469 g         4596 t
ORIGIN      
        1 tgcaaataat gtcatataag ttacacttta gttatcttag ttacaacttt gtcggtttac
       61 tttttctact ttcttcgata taattataat atatttttga tacaattcga caaaaggggg
      121 aatgctgtaa ttatggtaaa aaaaagaaaa aagaaaactg ttacacttac tatacttgtt
      181 gtaatagtta ttggtatttt ttctatggca tgggcggaca tgtctagaaa aaaagaaatg
      241 aaagaaactc aagaaagact taaagctgaa agattagaaa aagaaagaat tgaaaaagaa
      301 aaaaatgaac ctattattgg tgcaaaaaaa gaagctgtaa aagaatatgg atatgacgca
      361 aaaatagttt gggacaaact taataaatat gactattcaa acaatggaga aaagatagtc
      421 tttcttacat ttgatgatgg accatctact actaatactc cacaagttct agatatctta
      481 aaaagacatg gtgttagagg aactttcttt attaagggtg attctcttga aagaaaagga
      541 gctaatgaaa ttttaaaaag aacatttgac gaaggaaatg caatagccca tcactcttat
      601 actcatgact ataaaaaact atatcctagc agaagcctaa atctcgatac attcgtaaat
      661 gaacttaaca aaactgatga agctatgaaa aaagccttag gaaagaattt ttcctcaaat
      721 gtagttagat gcccaggcgg gtatatgtca tggaaaaata tggagcccct aggaaactac
      781 ttaaaagaga aaaacatggc atcaattgat tggaatgctt taaatgctga tgcagaaggt
      841 aaaaagaaaa atgcacaaga attatttgaa catgcaaaga aatcttctga aggcaaagaa
      901 atggttgttc ttttaatgca tgatacttat ggaaaacaag aaacagtaaa tgcattggac
      961 caaattataa catattttaa agacaatggt tatcaattta aaattttaat ataataaaaa
     1021 agcaagttta ttgaatatat attttatact caatagactt gcttttactt tactctattt
     1081 tactagctcg atttttacaa attttttctt tcctatttga acaactaatt cactctcttt
     1141 tacaatagtg cttaaatcct ctactttttc accatttacc tttactccac cttgagaagc
     1201 taatcttcta acttcactct tactcttaac aatctcatta gaaactagta cttcgattaa
     1261 gtcaaatcca tcttcctcaa cttgaattgt ttgtatatct tcaggtattt gacctttttg
     1321 gaatacagat ttaaatcttt cttcagcttc tctagcagat tcttctccat ggtataatgt
     1381 tactatttct cttgctaagt tcatctttat atctcttgga tttactgaac cctctttcaa
     1441 ctgagattct attttattaa cttcatcagg atgaacatct gttactaaat tgtagtattt
     1501 aattataagt tcatcaggaa tttccataga tttttggtac attattcccg cttcttcatc
     1561 tattccaata tagttaccta aactcttact cattttttct ttaccatcta atccttctat
     1621 taaaggcatc attattacta tttgagactc cataccaaat tctctttgta atgatcttcc
     1681 cattagtaaa ttgaatcttt gatcagttcc accaagttct atatcagctt ctaatgctat
     1741 agagtcatat gcttgcatta atgggtagaa gaactcatgt actgatattg gcatttgtcc
     1801 ttcatatctc tttttaaaat cttctctttc taacattctc gcaacagtaa tagttgctgc
     1861 taattttatt acatcttcaa aatttaactt ggctaaccac tcgctattaa atcttacaat
     1921 agttttttct ttatctaaaa ctttgaaaat ttgttcttca taagtttttg catttgcaag
     1981 aacttgctct gttgttaaag cttttctagc tttagatttt ccagttgggt ctccaatttt
     2041 tcctgtaaaa tctccaatta ttattactat ttgatgacct aaatcttgta attgtttcat
     2101 ctttctaaga actactgtat gccctaaatg tatatctgga gcacttgggt ctagacccaa
     2161 ttttactatc ataggctttc cttctttttc tgatttaata agcttttccc ttaactcttt
     2221 ctcatctatt aaatcatcta cacctttcat aattattcgc atttgctcgt ctatcgattt
     2281 catttttcat tcctcctaaa atatttatat aatctataat ttttattttt ataataaaaa
     2341 aagctttcat cctcttaaaa ggacgaaagc attgcttacg tgttaccacc ttagttcatc
     2401 aatatctcac gatattaatc tcttagagta caattacata tactcgtgta ctataacgtg
     2461 tactaatccg atatctctta atggttgtta tctttcagag atagtgctct gagatgtatt
     2521 caaactaaat caatttattc catttcacca aactggaact ctctgttaaa taaaatctag
     2581 ccctacttct tctcttcata gcatttatat attcagatta tttcatcaat tacaagaaat
     2641 ttttcttaac ttactcataa tatattatta tttttatatt ttgtcaacct tttttattat
     2701 tatatacatg tttttttaca ttatactact tttttatatt ttttaccaat acctaaagtt
     2761 tatttttcaa tcaaaagatt tatttgttta ttgactatat tactctgact caatcttata
     2821 tctattttct tatttaaaaa tgcgtttata attattaaac taataactgt tacatcaaat
     2881 ataaaaaaca gcaataaaat tattatcaaa aataaaattc tagcatataa taattttcta
     2941 aacttagcta ttataaagtt ttgtagtgac atacatgagt ctctaataat aaaatagaca
     3001 cttatatagg ttattgatat aaagattgtt tttaaataaa cagaatcaac tttaacattc
     3061 cacagtttta aaacttcaaa aatggctaga taagaaacac atatgtaaaa agcttttttt
     3121 gagcaacata aaaattggtt taaacttcta aaattcttaa atattaaaaa tttttttaaa
     3181 gaattagaat ctaaaatatg taatttattc cctaaaatta aacttaataa atatatgcaa
     3241 aacaccatac caaaaggaaa caatgctatc attaaataat aaataaaatt tgtataccca
     3301 ttaaaataaa tccatttgct atatccttga aattctttca taaaactata acctattaat
     3361 gttgaagcat atatatcaat gaaaaccatt acaataaccc caaatactga accatatact
     3421 aaatcatcca taaccattgt aggcttattt attagatagc cacacattat accaagtaat
     3481 acactctgac ttgcccaaat agcagttcct agatttccaa gtaccagaga aattattaaa
     3541 agcgaagtaa ttccagataa aatggtatac tttaaatcac atttcaagca gattatacaa
     3601 aaaaatactg gcactataaa atctaagtaa atagcatatc caaatccagt actcacagct
     3661 ataattgtta ctactatgaa taaagatgac aataaagctg cttcagttaa ttttttagta
     3721 gtattcaaaa cactgcctcc ataaatcata aattttctaa ctaaagtttt gtttttaata
     3781 tagtatatca ctttttaaaa atatttttaa taaatataaa ttttaataaa ggtatttatg
     3841 ttaaaaatgg tataatgtat cttaatattc agttttttaa gatgctagga ggtaattact
     3901 atgtttaaaa ttaaacactt taatgatttg agtttagatg aattttatga aatagcaaaa
     3961 tcaagatatg aggtatttgc ttgtgaacag aagatatttt cattaaacga ttatgatgat
     4021 atagataaaa gctcttatca catattttta aaagaaaatg gattgatttg tgcttatgct
     4081 agaataattc ctaaagaata cagctcttat aatgatgttt ccattggtag agttttagtt
     4141 ctttctagtc atagaagaaa aggcttagca aaacaaatga tggattgtgc tatagatttt
     4201 ataaaagtta acttacacga aaataatata accttatcag cacaaactta tataaagaac
     4261 ctttacttat cttgtggttt taaagaaatc tcagaagtat acgacgaagc aggtattgaa
     4321 catattaaaa tgagattata gtgttgacaa gctaatattg ttaatatata atttaagtat
     4381 aaattaaata ttgaattgat atttaggtat ttgaaaatac cttacataga gtcaggaggc
     4441 ttttgctttc tgactttttt attatcaaaa ttcaataatt atatggattc aaagtgttaa
     4501 tttttattct atattctact ttccccaaaa gtaagaatta tacccaactt tgtatgatag
     4561 gcatcttgcc tatctttttt ttgtaaaagt aacaaggtaa taatataatg catagtttag
     4621 aataagaaga ttagcccagg ctaaatttca agcatggagg tgctactatg aaaaacctta
     4681 acgatattca agtgaaaaaa actgtcaaag ttgaagatat attatctagt ggtaatttaa
     4741 gagaaagaat gttagcgctt ggtcttacta gaggagctgt tatagatgta gttagaaaag
     4801 gtccaaaaaa taatttaact gtctataaaa ttagaggtag caagatagct ctacgacaag
     4861 aagaaagtag cttaatatta gtttctgaca tttaaaaatt tctagcagga ggatattaat
     4921 atggggttga cacataactc aactaaaatg agttctttaa aagacatgtt tgatatagac
     4981 aataaggaag accagtttat aattgcattg gctggcaatc caaatacagg taaaagtact
     5041 gtatttaatc atcttacagg gcttagacag catactggaa actggccagg aaaaactgta
     5101 gctacagctc gtggtaattt taaatataaa aataccgaat atgcattaat agatttacca
     5161 ggaacatatt ctttatttgc cttatcacaa gaagaaattg tagctcgtga ttttatatgt
     5221 tttggtaatc cagatgcagt aatagtagtg tgtgatgcta catgtcttga aagaaattta
     5281 aatttagtat ttcaagtgat ggaacttaca gataaagtta ttctgtgtat taatttaata
     5341 gatgaagcta gaaaaaaagg cattactatt gataaaaaac ttttagagga ttctcttggt
     5401 attccagtta ttttaactgc tgcaagaaat ggctctggta tggatgaact attggatact
     5461 ttaaatgatg tatcttttga caaatataaa ttaaataata aacctgttag atacaatgaa
     5521 aatatagagg atgtggtaaa atcaattcaa ccagaattgg ataatattat accaggtata
     5581 aattctagat ggttgggact tagacttatt gatggagatg aaagtatatt tgaatctatg
     5641 agtaattata tagacaaaga ttctatagat gcgattaatg aagtgaagaa aaagattcca
     5701 gataatataa ataaacagaa aataagagat gagtttacta aaatcaacta tgattatgct
     5761 aaaaaactga gtgatgagtg ttgttctaat gttgctaaaa aatcaactga tagagaagaa
     5821 aaagtagata aaatattaac atctaaaata tttgggcttc caataatgct cttattatta
     5881 ggtactatat tgtggattac tatagaaggg gctaactatc catccacttt attatctaat
     5941 ttattactcg gatttgagcc aagtatttcg ggcatattaa atagtattaa ttgtccatca
     6001 tggttaaatg atatgttggt tttaggatta tacagaacat tagcatgggt aatctctgta
     6061 atgctacctc ctatggcaat attttttcca ttatttacct tactcgagga ttttggatat
     6121 ttgccaagag ttgcatttaa cttagaccac ctatttaaaa aagcttgtgc acatggaaaa
     6181 cagtgtttga ctatgtgtat gggatttgga tgtaatgcag ctggagttat tggttgtagg
     6241 ataattgact ctccaaggga aagacttata gctatactta caaacaactt tgttccttgt
     6301 aatggtcgtt ttcctacttt gatagctata tctacaatat tttttagctc agttataacc
     6361 aactctttcg tatcaagcgt agcaactgca ctttgcataa ctttactgat aattctaggt
     6421 gttataataa cattgttggt ttcttatact ctttctaaga cgctattaaa aggagttccg
     6481 tctacattta cattagaact tcctccttat agagttcctc aaataggaag aactttatat
     6541 acctctataa tagatagaac aatatttgtt ttaggaaggg ctgtaatggt agccatacct
     6601 gctggtgtca taacttggat atttgcaaat atatatatag gagatttaag tatattatca
     6661 catgttgcaa actttttaga ccctttagct aaattaattg gtcttgatgg ttttatatta
     6721 cttgctttta ttcttggatt cccagcaaat gagatagttg tacctatact tttaatggct
     6781 tacctagcaa ctggttctat gatagaatta gatagcttta gtgctttagg tcaagttctt
     6841 agagaacatg gatggactta tttgacagct ttaaatgtta tgctgttcag tttactgcat
     6901 tggccttgtg caacaacact tttaacaata aaaaaagaaa ctggtagttt aaaatggact
     6961 gccttaggat tcttaatgcc tactatactt gcttttgttg tttgtttttt aacaactaca
     7021 gtttacaatt tatttatata attagtatta actgacagta gacatatatt agaaaaacta
     7081 tttaaaaatt tatatacttt attataaagc atagagatat attcaatgat aagtaaaatt
     7141 acttaacatt tgaatatatc tctattttaa ttcttatctg gatacttgga ttgaaatttc
     7201 agtcatataa ttatcttcat tactttcaca aaaaaagtca agtatactct cctcatacga
     7261 atttccaata attttatatt cattttcata tatatatttt ttcaattttt cgtaagactt
     7321 atatgaatcc tcatattttc caatatgttt tatgcaagca tacattcctt ctggctttac
     7381 atatagcttt ttatccttta tttttttatc tatcttcaaa taaaaacttg atatacttaa
     7441 atagtcccca ttttttatat tttcattact tactattact cccaactcat atccttgata
     7501 caaatttttc gatttacaat aattggtgaa atctattaat tccatcataa aatcactatt
     7561 actcatatta ctcaaatcgc ttgatagaac taaatattct tcaaactttt tggatatata
     7621 aatttcttca ctattcattt tagaaattcc ttcaactgtt atacttattc tattttgcat
     7681 cttagaactt atctttctta gtttttttat tttttctctt atcttttttt cttcttcttt
     7741 aagcattttc acacaaagct ggggagttct attttgtata tactctttta tttcctctaa
     7801 actcattcct atttctctta atgattgtaa tatatcaagt gtatcatatt gataaactga
     7861 gtaatatcta taattatttt tatctttata ttcaggagaa aaaataccta ttttatcata
     7921 gaaaattaat gtctgcttag atataccaca caatttagca aattcacctg ttgtaaaata
     7981 ctgtttttcc aagggttatg cactcctttt ctttttgttg tctatatagt tactatattc
     8041 tttattattg actaaatcaa gtagttatta ttttagaaaa tcaatatggt tttgatttta
     8101 aaggttaaat gtcagttaga tgtcagttat aaatatttta tccattctca tcataaaaaa
     8161 gatataccct cttaactttt ggatggaata ttatatttat gcttgttaga gtggaagttg
     8221 taataggtgc tacaataaga tatttaatta aacataaagt taattattgt aataaaaaag
     8281 ccttagataa attagagtta aagtatgaaa tatagataga gaaaaagctg ttaaactaga
     8341 aatattttat caatatttaa tagaacttga atatatagaa atgagtttat taaataataa
     8401 gattagatac tgcaataaca tcattaacat tagtatctat tataacaata gcatcatatt
     8461 atttaatcag aaaaaatgtg taattatata agctatttat aataaaacac tgacaaaatt
     8521 aataataaaa aggtaatagg atttctatac taccttttgg tttttataat atagtgtaat
     8581 tttattatat ttttgtatgc taatataact agataatcat aagataacac attaaaagaa
     8641 aattatattc tgtatagtta gtataaaacg taagtaatat aaatttatgt aaagcaacaa
     8701 aagatggatt taaattccat atagttaata taaaacaaat tttgtatcct tgacattgac
     8761 atctaatata taatttacat tccatatagt taatataaaa cgctactacg tcaactattt
     8821 caagagtttc atttaaaatt tacattccat atagttaata taaaactatt cctcgacacc
     8881 agggaaatta aacccttatt ttatttacat tccatatagt taatataaaa cttaaagtta
     8941 tagatgaaga atttttatgt aataacatat ttacattcca tatagttaat ataaaacgca
     9001 tttaaagaga ttttatctaa atatgaaatg gataatttac attccatata gttaatataa
     9061 aacccatttc catatcactg tctttatact tatttccaaa tttacattcc atatagttaa
     9121 tataaaacaa ttttagcaag gaaaaagtga ttgaaaagcg taagagattt acattccata
     9181 tagttaatat aaaactgctg tttgggatag caaagaaatt aatgaggatg taatttacat
     9241 tccatatagt taatataaaa ctagaaaaat actttgtaat aagtcgctac tatgacaaat
     9301 ttacattcca tatagttaat ataaaacccc aaaataaact tagtattttc aatacttaca
     9361 cgtatacaac tctcttaaat ttgcagtgaa cgagcaatag tgcaattgat aacgcttatc
     9421 atgcactctc aacaccttat atttcaactg ttacgccaaa ttttttcaca aatatcgctc
     9481 actgcaaaat ttctataatt ttattatatc ataaatatat tacttttgaa tattgatacc
     9541 aatttatacc ataataaaaa caagaaaaaa cgtaatctat ctacattaga tttaaattct
     9601 ataacttaaa gttttttaca taatatttat gcttgtttaa tcaatttaat atatcattct
     9661 tgtatattgc ttttaatggt cttttttctt aaataaatat ctatctgaac aagcatagtt
     9721 taattaaaaa cttttgtatt cacttaaaac tgtatctacc tcttattatt ttatttatga
     9781 tatattattt ttaatctaaa aatagatatc tatttttatt atggttatcc ctttttagaa
     9841 tactttaatc atatactatt cctaatttaa catccattga aatgctattt tcttatatta
     9901 caatttataa ttgatatatc ttcaatataa taatttaatg tatttttgat tttaaagctt
     9961 tttagtaata ttattgataa attagaatta attatctagc tttaaaaggt caaagatttc
    10021 tgttgctgtc tgcggttttc caaataaata tccctgtatg taatctggct ttatttgttt
    10081 aactaaatca tactcttctt gtgtttctac accttctaaa catactttta ttcctacatc
    10141 atgacaaatt gcaacaataa aatgtataaa tgtagcatca aacttactct ttaatatatc
    10201 tttaacaaat actctatcaa ttttcactat atctattgga gaaaatttta aaacttctaa
    10261 agacgaatat ccagtaccaa aatcatccat agcaatataa atgcctaagt cttgtaaagc
    10321 cttaaattta gactgtaaaa tactcagatt ttgtactgta tgactttctg tcaattctaa
    10381 aacaagattt tgataaggaa aattttcatc atctataatt tttactatat ctttaataaa
    10441 tgtatcttct aatatttgaa cagctgaaac gtttacactc actttaaaaa aaggataatt
    10501 atcaatccat ttcctaaaag tacgtaatgc cattcgaagc acccaagccc ccacaatatt
    10561 tatcatatca ttttcttcta atattggaat aaattccaaa ggagaaattg ctttatattt
    10621 gtcattagtc caccttaaaa gaacttctac acctatcatt ttatgacttt gtgtatctac
    10681 ttgtggctgg aatcgaagac taaatcccct aaaatcatca ttaattgatg cctttaaatc
    10741 ccgcatcatt tctaatgaat acattttatt ttttaatatt tcttgtgaga aaaaaacaag
    10801 cctgttttta ccgtgctctt tcgcatattg taaagagtaa ctagcgcatt tataaagttc
    10861 ttttactgta tctccatgct ttggatatat tacacagcca gcagaaattg ttatatttaa
    10921 tccatatttt ctccatagtt gaagatgtat aatcatattc tgtatttgat catataaagt
    10981 tagaatttca ttttcttcaa cattatcaac aagaatgccc atttcgtctc catcaagttt
    11041 ataaagttca gcattatctg gtaaaataga ttgtatagac tgtgctagtg tttttattat
    11101 attatctcca aaatccctat catacatctc attaacattt ttaaaatcgt caatatttaa
    11161 taatactatg cataattttt caatcatagg atttgaaatt ttatcttcaa atacactcat
    11221 aaattcatga taattcagta actgtgtcaa tgaatcaatc ttattttgtt tacctaattg
    11281 cgtcattatt cctgcaaaaa tacttggttc tccaaattca tcacgcatta gttgccctct
    11341 acatcttaac catatatatt ctccactacg atttttagcc ctaaattcca ctgtatgata
    11401 atccatctgg tttttaccta tttcagtgtt tgacttataa aaacgattcc agtcttctgg
    11461 atgtacaatt ttttcccagt aaataagcgg attttcaaca atttctcctg gtaagtcaaa
    11521 taattcaact tgtgatggtg aatatcgaaa tactcctgtt ttcatattac aaatgtatat
    11581 atattcatct gtacctctaa tcaaagcatc ataaagcaat gtagaattat aatcaaaatt
    11641 gtctcttgaa aaattaaaaa ctcctttttc ttctttagta tttgatattt cattaaattc
    11701 attaatctta attgctttat ttaagattgt tgagtttgca atatctattg attgatgcat
    11761 atgaacaact gtgccatctt gccatgtaat taaactatca taattttcaa aagcacgatt
    11821 taacttatta catttttcat accactttat taatactcct tttttatcat tctttaataa
    11881 ttctaaaact ttacaaaaac tacaagtaga attttgctca ggatataata cttgccagca
    11941 aacttttcct tctgggtcta aaatattata ttcttctttc attttcttgt tcataaaaat
    12001 aatttcatta gtatgaatat ttgtaacata tatattaatc tgtagttgat ttaatataac
    12061 ttcaaaatta cgtttgttca tatacttatc tcctccatag ttactgctct ttataacaac
    12121 actgttaaaa ttattctttt tagaagattt acttataatt tat
//
LOCUS       DAEPXF010000110        12153 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain HB27
            SAMN13376389-rid12479423.denovo.116, whole genome shotgun sequence.
ACCESSION   DAEPXF010000110 DAEPXF010000000
VERSION     DAEPXF010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13376389
            Sequence Read Archive: SRR10518432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 12153)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12153)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001069624.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001069624.1
            Genome Coverage       :: 315x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/15/2021 01:54:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,165
            CDSs (total)                      :: 4,076
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 89
            rRNAs                             :: 10, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 10 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 19 of 69
            Pseudo Genes (incomplete)         :: 44 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 10 of 69
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12153
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13376389-rid12479423.denovo.116"
                     /strain="HB27"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="China: Hebei"
                     /collection_date="2017"
     gene            160..948
                     /locus_tag="KRL67_002606"
     CDS             160..948
                     /locus_tag="KRL67_002606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HBH1523473.1"
                     /translation="MEKLQGKIAVVTAATKGIGLASAEILAKNGATVYLAARSEELAN
                     EVINKISAEGGCAKFVYFNAREEETFTSMIEEVVKKEGKIDILVNNFGSTNPSLDKDL
                     VSGDTDNFFDTVNTNLKSVYLPCKAAIPHMIKNGKGSIVNISSIGSVLPDLSRIAYCV
                     SKAAINSLTQNIATQYAKDNVRCNAVLPGLIATKAALDNMSPEFIKEFLKHVPLNRIG
                     EPDDIAKAVLFYASDDSSFITGDLLEVAGGFGLPTPQFADNILG"
     gene            1419..5135
                     /locus_tag="KRL67_002607"
     CDS             1419..5135
                     /locus_tag="KRL67_002607"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HBH1523474.1"
                     /translation="MPHPVTIGKRTRMSFSKIKEIADVPNLIEIQVDSYEWFLKEGLK
                     EVFDDISPIEDYTGNLILEFVDYSLDDKPKYDIEECKERDATYCAPLKVKVRLINKET
                     GEIKEQEVFMGDFPLMTERGTFVINGAERVIVSQLVRSPGVYYAEERDKTGKRLISST
                     VIPNRGAWLEYETDSNDVISVRVDRTRKQPVTVLLRALGIGTDAEIIDLLGEDERLSA
                     TLEKDNTKTVEEGLVEIYKKLRPGEPPTVESASSLLNALFFDPKRYDLAKVGRYKFNK
                     KLALCYRIMNKISAEDIINPETGEVFVKAGEKISYDLAKAIQNAGINVVNLLMDDDKK
                     VRVIGNNFVDIKSHIDFDIDDLNIKEKVHYPTLKEILDGYSDEEEIKEAIKSRIKELI
                     PKHILLDDIIASISYEFNIFYNIGNIDDIDHLGNRRIRSVGELLQNQVRIGLSRMERV
                     IKERMTVQDMEAITPQALVNIRPVSAAIKEFFGSSQLSQFMDQTNPLSELTNKRKLSA
                     LGPGGLSRERAGFEVRDVHHSHYGRMCPIETPEGPNIGLINSLGTYAKINEFGFIESP
                     YRKFDKETSTVTDEIHYLTADEEDLFVRAQANEPLTEDGKFVNHRVVCRTVNGAVEMV
                     PESRVDYMDISPKQVVSVATAMIPFLENDDANRALMGANMQRQAVPLVRREAPIIGTG
                     IEYRAAKDSGAVVVARNSGIAERVTADEIIIKREDGNRDRYNLLKFKRSNSGTCINQT
                     PIINKGDQIMKGDVIADGPATDLGEVALGRNCLIAFMTWEGYNYEDAILINERLVKED
                     RLSTIHIEEYECEARDTKLGPEEITRDIPNVGDSAIKNLDDRGIIRIGAEVDSGDILV
                     GKVTPKGETELTAEERLLRAIFGEKAREVRDTSLKVPHGESGIIVDVKVFTRENGDDL
                     SPGVNELVRCYIAKKRKIKVGDKMAGRHGNKGVISRVLPEEDMPFMENGTPLDIILNP
                     QGIPSRMNIGQVLEVHLGLAAKTLGWYVATSVFDGANEYDIMDALEEAGYPRDGKLTL
                     YDGRTGESFDNRITVGYMYYLKLHHLVDEKLHARSTGPYSLVTQQPLGGKAQFGGQRF
                     GEMEVWALEAYGAAHILQEILTVKSDDVVGRVRTYEAIVKGENIPEPGIPESFKVLIK
                     ELQSLCLDVKVLTDEDQEIEVRESVDEDDTIGEFELDVVNHMGEVEESNIIEEIEDDF
                     AENAEDEDIENLEEFTEDDLFEEEIDFDSDDFDM"
     gene            5177..8662
                     /gene="rpoC"
                     /locus_tag="KRL67_002608"
     CDS             5177..8662
                     /gene="rpoC"
                     /locus_tag="KRL67_002608"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HBH1523475.1"
                     /translation="MFELNNFESIKIALASPEKIRQWSRGEVKKPETINYRTLKPEKD
                     GLFCERIFGPQKDWECHCGKYRRVRYKGVVCDRCGVEVTKSKVRRERMGHIELAAPMS
                     HIWYFKGIPSRMGLLLDMSPRSLEKILYFASYVVVDPGETGLNEKQLLTEKEYRTALE
                     KYGYTFTVGMGAEAVKTLLQNIDLEQQSKDLRAELKDSTGQKKVRTIRRLEVVEAFKK
                     SGNKPEWMILDAIPVIPPDLRPMVQLDGGRFATSDLNDLYRRVINRNNRLKRLLELGA
                     PDIIVRNEKRMLQEAVDALIDNGRRGRPVTGPGNRPLKSLSDMLKGKQGRFRQNLLGK
                     RVDYSGRSVIVVGPELKFYQCGLPKKMALELFKPFVMDKLVKEGYAHNIKSAKSIVEK
                     VKPEVWDVLEDVIKSHPVLLNRAPTLHRLGIQAFEPILVEGKAIKLHPLVCTAYNADF
                     DGDQMAVHVPLSVEAQAEARFLMLSVNNILAPKDGSPITTPSQDMVLGCYYLTIEAQD
                     GAKGTGMVFKDFNELLLAYYNKSVHLHALVKLKVTLEDGRSSLVESTVGRFIFNENIP
                     QDLGFVDRKENPFALEVDFLADKKSLGKIIDKCFRKHGNTETAELLDYIKALGFKYST
                     LGGITVAVDDMSVPEEKKVFIAEAEAKVDKYEKAYRRGLISDEERYEKVIETWTETTD
                     KVTDALMGGLDRLNNIYIMAHSGARGSKNQIRQLAGMRGLMANASGKTVEIPVKSNFR
                     EGLSVLEYFTSSHGARKGLADTAIRTAESGYLTRRLVDVSQDVIVREIDCGTEDTTEI
                     YAIKEGNEVIEEIYDRIVGRYTIDPILNPETGEVIVEADSMIQEDEAETIVALGIEKI
                     RIRTVLNCKTNHGVCSKCYGRNLATGKEVNIGEAVGIIAAQSIGEPGTQLTMRTFHTG
                     GVAGADITQGLPRVEELFEARKPKGLAVITEVSGRVEIDETGKRKEVNVIPEEGETQT
                     YVIPYGSRLKVKQGQMLEAGDPLTQGFINPHDIVRVNGVKGVQEYIVKEVQRVYRLQG
                     VDVNDKHIEVIVRQMLSKVKVEDPGDTDLLPGGYEDVLTFNECNKDAIDKGLRPAVAK
                     RVLLGITKASLATDSFLSAASFQETTRVLTEAAIKGKEDHLIGLKENVILGKLIPAGT
                     GMKKYRNIAVEKIED"
     gene            8951..9373
                     /gene="rpsL"
                     /locus_tag="KRL67_002609"
     CDS             8951..9373
                     /gene="rpsL"
                     /locus_tag="KRL67_002609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001142340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HBH1523476.1"
                     /translation="MPTINQLVRKSRKALEKKSTAPALQKGYNSLNKKVTDASAPQKR
                     GVCTSVKTVTPKKPNSALRKVARVRLTNGIEVSAYIPGEGHNLQEHSVVLIRGGRVKD
                     LPGVRYHILRGTLDTAGVDKRRQSRSKYGAKRPKEAKK"
     gene            9496..9966
                     /gene="rpsG"
                     /locus_tag="KRL67_002610"
     CDS             9496..9966
                     /gene="rpsG"
                     /locus_tag="KRL67_002610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001137493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HBH1523477.1"
                     /translation="MPRKGNIPKREVLPDPMYGSKVVTKLINNLMVDGKKGKSQRIVY
                     DAFAIVAEKTGEEALEVFNKAMDNIMPVLEVKARRVGGANYQVPIEVRPERRQTLGLR
                     WLVKYTRARGEKGMVEKLAKEIMDAANNTGASVKKKEDTHKMAEANKAFAHYRW"
     gene            10015..12081
                     /gene="fusA"
                     /locus_tag="KRL67_002611"
     CDS             10015..12081
                     /gene="fusA"
                     /locus_tag="KRL67_002611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HBH1523478.1"
                     /translation="MARKFPLEKTRNIGIMAHIDAGKTTTTERILFYTGQTHKIGETH
                     EGASQMDWMEQEKERGITITSAATTASWKDHRINIIDTPGHVDFTVEVERSLRVLDGS
                     VAVFCAKGGVEPQSENVWRQAETYGVPRIAFINKMDILGADFYNVISMMKSRLNSNAV
                     PMQLPIGKEDSFIGIIDLLKMDAVIYKDDLGVEMEETDIPEDMKELAAEWREKLVESV
                     AETDEELMMKYLEGEELTIDELKVAIRKATIACEMNPVFCGTAYRNKGVQLVIDAVLD
                     YLPAPTDIPAIKGILADGEEAERHSSDEEPFSALAFKIMTDPFVGKLAFFRVYSGTLE
                     SGSYVLNATKNKRERIGRILQMHANTREEITKVYAGDIAAAVGLKDTTTGDTLCDPAN
                     PIILESMEFPEPVISVAIEPSSKAAQEKMGIALQKLAEEDPTFTVKTDQETGQTIISG
                     MGELHLEIIVDRLLREFKVEAKVGAPQVAYRETITQPVDVEYKYSKQSGGRGQYGHVK
                     IRVAPQEPGEGYKFTNKTVGGSVPKEYVGPVDMGIQGAMQSGIVAGYPVVDVAVELYD
                     GSYHEVDSSEMAFKMAGSMAFKDAMKKGNAVLLEPYFKVEVVTPEDYMGDVMGDLNSR
                     RGLIQGMEARSGAQVINAFVPLSEMFGYSTDLRSSTQGRATYTMIFDHYEQVPASVAK
                     KIAEGK"
BASE COUNT         4701 a         1543 c         2500 g         3409 t
ORIGIN      
        1 tgtaaaaatt tacatattat tttaaaaaaa tacacgagtg ttttataaat aaaatgtaat
       61 aaataattaa ttaacaaata taaattttaa aataagttag atatttagaa aaatcgaaat
      121 ttgtaggatt taaaggacat atacaaggag gagttaataa tggaaaaatt acaaggaaaa
      181 attgcagtag ttactgcagc aacaaaaggt attggattag catcagcaga gatattagca
      241 aaaaatggag caactgtgta tttagcagct cgttcagaag aattagctaa tgaagttata
      301 aataaaataa gtgcagaagg tggttgtgct aagtttgttt actttaatgc tcgtgaagaa
      361 gagactttta cctcaatgat agaggaggta gttaaaaaag aaggtaagat agatatactt
      421 gtaaacaatt ttggttcaac aaacccttct cttgacaaag accttgtaag tggggataca
      481 gataatttct ttgacacagt aaatactaat ttaaaaagtg tgtatttacc atgtaaagca
      541 gcaattcctc atatgataaa gaatggaaaa ggcagtatag taaatatatc aagtataggt
      601 tcagtgttac ctgatttatc tagaatagct tactgtgtat caaaagcagc aatcaactca
      661 ttaactcaaa acatagctac acaatatgct aaagacaatg ttagatgtaa tgctgtactt
      721 ccagggctta ttgcaactaa ggcagcatta gacaatatgt ctccagagtt cataaaagaa
      781 tttttaaagc atgtcccttt aaatcgtata ggagaaccag atgatatagc aaaagctgtt
      841 ctattctatg ctagtgatga ctcatcattt ataacagggg atttacttga agttgcagga
      901 ggatttggtt tacctactcc acaatttgca gataatatat taggataatt aaattaataa
      961 tatgcaatga aagcttatgt attagttata gtataaagga tatttttata aagaccatag
     1021 atataattta tgttggaaat atttgaggtt caaaattcca agttaaattt atttatggtc
     1081 tttattttgt atattttaat aaattgtata tatttgtata aatgtaatta tttacaatca
     1141 ttttaatatg gaattataat taaatagggg ggatggtatt tgtataaaat tttatataga
     1201 aaattagacc tataaatgaa aaatatggat tttatattgg aaaattgtac ataatacata
     1261 atatatgctt aaaataattt acaaaaaatc aaaaataatg catagtttac atataagata
     1321 aaaaatatat tgaaaaatac ttgacattat gctaaaatta ttagatgcgt tagaatgaac
     1381 tattgtttta tttttaatta ttgagaggtg aaaagtgaat gccacatcct gtcacgatag
     1441 gtaaaagaac tagaatgagc ttttctaaaa ttaaggaaat agctgatgtg ccaaatctta
     1501 tagaaattca agtagattcc tatgagtggt ttttaaaaga aggtttaaaa gaagtgtttg
     1561 atgatatttc accaatagaa gattatactg gtaatcttat attggaattt gtcgactatt
     1621 ctctagatga taagccaaag tacgatatag aagagtgtaa agagagagat gctacatatt
     1681 gtgcacctct aaaggttaag gtaagactta taaataaaga aacaggtgag ataaaagaac
     1741 aagaagtgtt tatgggtgac ttccctttaa tgacagaaag aggaactttc gttataaatg
     1801 gagcagaaag agttatagta agtcaattag ttagatctcc tggtgtttac tatgcggaag
     1861 aaagagataa aacaggtaag agattgattt catctactgt aattcctaat agaggagcat
     1921 ggttagaata tgaaacagat tctaatgatg taatatctgt aagagttgat agaacaagaa
     1981 aacaaccagt tacagtttta cttagagctt taggaatagg aacagatgca gagataattg
     2041 accttttagg agaagatgag agattatctg ctacattaga aaaagataat actaagacag
     2101 tagaagaagg tcttgtagag atatacaaaa aattaagacc aggtgaacct cctactgtag
     2161 aaagtgcatc atctttatta aatgctttat tctttgaccc aaaaagatat gacctagcaa
     2221 aagttggtag atataaattc aataagaaac ttgccttatg ttatagaata atgaataaaa
     2281 tttcagcaga agacataata aatccagaaa caggagaagt atttgtaaaa gctggagaaa
     2341 aaataagcta tgatttggct aaagccatac aaaatgcagg tataaatgta gtaaatctac
     2401 taatggatga tgataaaaaa gttagagtta taggaaataa ttttgtggat attaagtctc
     2461 acattgattt tgacatagat gatttaaaca taaaagaaaa agtacattat ccaactttaa
     2521 aagaaatttt agatggatat agtgatgaag aagaaataaa agaggctata aaatcaagaa
     2581 ttaaagaact tataccaaaa catatattat tagatgatat aatagcttca ataagttatg
     2641 agttcaatat attctataat ataggaaata ttgatgatat agatcaccta ggaaacagaa
     2701 gaataagatc tgtaggtgaa ttgttacaaa atcaagttag aattggtctt tcaagaatgg
     2761 aaagagttat aaaagaaaga atgacggttc aagatatgga agctataacg cctcaagcgt
     2821 tagttaacat aagaccagtt tcagctgcaa taaaagaatt ttttggtagt tctcagttat
     2881 ctcagttcat ggatcaaaca aatcctctat ctgagttaac gaataagaga aaattatcag
     2941 cccttgggcc tggaggtctt tcaagagaaa gagctggatt cgaagtgcgt gacgttcacc
     3001 attcacacta tggtagaatg tgtccaatag agactccaga gggaccaaat atcggtctta
     3061 taaactctct aggaacttat gcaaaaataa atgaatttgg atttatagaa tcaccatata
     3121 gaaaatttga taaagaaaca tcaacagtta ctgatgaaat acattattta actgctgacg
     3181 aagaagattt attcgtaaga gctcaggcaa atgagccatt aacagaagat ggtaaattcg
     3241 taaaccatag agttgtttgt agaactgtaa atggtgctgt tgaaatggtt cctgaaagta
     3301 gagtagatta catggatata tctcctaagc aggttgtatc tgttgccact gctatgatac
     3361 ctttcttaga aaatgatgat gccaaccgtg cccttatggg agcgaacatg cagcgtcagg
     3421 cagtgcctct agttagaaga gaagcaccaa ttataggaac tggtatagaa tacagagcag
     3481 caaaagactc tggagcagtt gttgttgcta gaaattctgg tatagcagaa agagtaacag
     3541 cagatgaaat aattataaaa agagaagatg gaaacagaga tagatataat ctacttaagt
     3601 ttaaacgttc aaactcaggt acttgtataa atcaaacacc tataataaat aagggtgacc
     3661 agataatgaa aggtgatgtt atagcagatg gtccagcaac tgatttagga gaagttgcac
     3721 ttggaagaaa ctgtcttata gcattcatga cttgggaagg ttataactac gaggatgcca
     3781 tattaataaa tgaaagatta gttaaagaag atagattatc aacaattcat atagaagaat
     3841 atgaatgtga agctagagat acaaaactag gaccagagga aataactaga gatataccta
     3901 atgttggaga tagtgcaatt aagaacttag atgacagagg tataataaga ataggtgcag
     3961 aagtagactc aggtgatata ttagttggta aagtaactcc aaaaggagaa actgaactta
     4021 ctgctgagga aaggttactt cgtgcaatat ttggagaaaa ggctagagaa gttagagata
     4081 cttcacttaa agtgcctcat ggtgaatctg gtataatagt tgatgtaaaa gtatttacta
     4141 gagaaaatgg agatgattta tctccaggag taaatgaatt agtaagatgc tatatagcta
     4201 aaaagagaaa aataaaagtt ggagataaaa tggctggtcg tcatggtaat aaaggggtta
     4261 tctcaagagt attacctgaa gaagatatgc cattcatgga aaatggaaca ccactagaca
     4321 taatacttaa cccacaaggt ataccatcac gtatgaacat tggtcaggtt ttagaagttc
     4381 atttaggact tgctgctaag acattaggat ggtatgtagc aacatcagta ttcgatggtg
     4441 ctaatgagta tgacataatg gatgctcttg aagaagctgg gtatcctaga gatggtaaat
     4501 taactttata tgatggtaga acaggtgagt cttttgacaa cagaataact gttggatata
     4561 tgtactactt gaaactacat cacttagtag atgaaaaact acatgcaaga agtactggac
     4621 catactcatt agttactcaa cagccactag gtggtaaagc acaatttggt ggacaaagat
     4681 ttggagagat ggaggtttgg gctctggaag catatggagc tgctcacata cttcaagaaa
     4741 tacttactgt taagtctgat gacgttgtag gtcgtgttag aacatatgaa gctatagtta
     4801 aaggtgaaaa tatacctgaa ccaggaatcc cagaatcatt taaggttctt ataaaagaac
     4861 ttcaaagttt gtgcttagat gtaaaagtat tgacagatga agaccaagaa atagaggtaa
     4921 gagaatctgt ggatgaagat gatacaatag gtgaatttga gctagatgtt gtaaatcata
     4981 tgggagaagt tgaagaaagt aacataatag aagaaattga agatgatttt gcagaaaatg
     5041 cagaagatga ggatatagag aatttagaag aattcactga agatgattta tttgaagaag
     5101 aaatagattt tgatagtgat gactttgata tgtaaattta attaatgtaa atctcaaata
     5161 gaagggagag aactccttgt ttgaattaaa caatttcgag tcgataaaaa tagcattggc
     5221 ttctccagaa aaaataagac aatggtctag gggagaagtt aaaaagccag aaactataaa
     5281 ttaccgtact ttaaaaccag aaaaagatgg tcttttctgt gaaagaatat ttggaccaca
     5341 aaaagactgg gagtgccact gtggtaaata tagaagagtt agatataaag gtgtagtttg
     5401 tgatagatgt ggagtagaag taactaaatc aaaagtaaga agagagagaa tgggacatat
     5461 agagctagct gctcctatgt ctcacatctg gtacttcaaa ggtataccaa gtagaatggg
     5521 acttttactt gatatgtcac caagatcgtt agaaaaaata ttatactttg cctcatatgt
     5581 agtagttgat ccaggagaaa ctggattaaa cgaaaaacaa ttgcttacag aaaaagaata
     5641 cagaactgct cttgaaaagt atgggtatac ttttactgta ggaatgggtg ctgaagctgt
     5701 aaagacatta ctacaaaata tagatttaga gcaacaaagt aaagacctaa gagcggagtt
     5761 aaaagatagt acagggcaaa agaaagttag aacaataaga agattagaag ttgtagaggc
     5821 atttaaaaag tctggaaata aaccagaatg gatgatttta gatgcaatac ccgtaatacc
     5881 accagattta agaccaatgg tacaacttga tggtggaaga tttgcgactt cagacctaaa
     5941 tgacttatat agaagagtta taaatagaaa caatagactt aaaagattat tagagcttgg
     6001 agctccagat ataattgtaa gaaacgaaaa aagaatgctt caagaagcag ttgatgcgtt
     6061 aatagataat ggtagaagag gtagacctgt aacaggtcct ggaaatagac cacttaaatc
     6121 tttatcagat atgctaaaag gtaaacaagg tcgtttccgt caaaacttac ttggtaagcg
     6181 tgttgactac tcaggacgtt ctgttatagt tgttggacca gagcttaagt tctatcaatg
     6241 tggacttcca aagaagatgg cattagagct attcaagcca tttgttatgg ataaattagt
     6301 taaagaagga tatgcacata atataaaaag tgccaaatct atagtagaaa aagttaagcc
     6361 agaagtttgg gatgttttag aagatgttat aaaaagccat ccagttcttc ttaaccgtgc
     6421 gccgactctg catagattag gtatacaagc atttgaacct atcttagttg aaggtaaagc
     6481 tataaagcta catcctcttg tatgtacagc ttacaacgca gactttgatg gtgaccaaat
     6541 ggcagtccat gtacctttat cagtagaagc gcaagcagaa gcaagattct taatgctttc
     6601 tgtaaataat atacttgctc ctaaagatgg ttcacctata actactccat ctcaggatat
     6661 ggttttaggt tgttattatc taacaataga agcacaagat ggagctaaag gaacaggtat
     6721 ggtatttaaa gactttaatg aactattact tgcttattac aataagtcag ttcatctaca
     6781 tgcattagta aaactaaagg taacacttga agatggaaga agttcattgg ttgaaagtac
     6841 tgttggtaga tttatattta atgaaaatat acctcaagac ttaggttttg tagatagaaa
     6901 agaaaaccca tttgctcttg aagttgattt cttagcagat aaaaagtctc ttggtaaaat
     6961 aatagataaa tgctttagaa aacatgggaa tacagaaaca gctgaattac tagattatat
     7021 aaaagctcta ggatttaaat attctacatt aggtggtata acagttgctg ttgatgatat
     7081 gagtgtccca gaagaaaaga aagtatttat agctgaggca gaagctaaag ttgataaata
     7141 tgaaaaagca tacagaagag gtctaatctc tgatgaagaa agatatgaaa aagttataga
     7201 gacatggaca gaaacaactg ataaagttac tgatgctctt atgggtggac tagatagatt
     7261 aaataatata tatataatgg cacattcagg agccagaggt tctaaaaacc aaattagaca
     7321 gctagcaggt atgcgtggtc ttatggcaaa tgcatctggt aaaacagttg agataccagt
     7381 taaatctaat ttccgtgaag gtttatcagt actagaatac tttacatctt cacatggtgc
     7441 cagaaaaggt cttgccgata cagctatacg tacagctgaa tctggatatt taacaagaag
     7501 acttgttgat gtaagtcaag atgttattgt aagggaaata gattgtggaa cagaagatac
     7561 tacagagatt tatgctataa aagaaggaaa tgaagttata gaagagatat atgatagaat
     7621 tgtaggaaga tatactatag accctatatt aaatcctgaa actggagaag ttatagttga
     7681 agctgattca atgatacaag aagatgaagc agaaactata gtagctttag gaattgaaaa
     7741 aattaggata agaacagttc ttaactgtaa aactaatcat ggagtttgtt ctaagtgtta
     7801 tggtagaaac ttggcaacag gaaaagaagt taatataggt gaagcagttg gtataatagc
     7861 agctcaatct atcggtgagc cgggtactca gcttacaatg cgtacattcc atactggagg
     7921 agttgcagga gctgatataa ctcaaggtct accaagggtt gaagaattat ttgaagctag
     7981 aaaaccaaaa ggattagctg taataactga agtatctggt agagttgaaa ttgatgaaac
     8041 aggaaagaga aaagaagtaa atgtaatacc agaagaaggc gagactcaaa catatgtaat
     8101 tccatatggt tctagattaa aagttaagca aggtcaaatg ctagaggctg gagaccctct
     8161 aacacaagga tttataaatc ctcatgatat cgtaagagta aatggtgtta agggagttca
     8221 agaatatata gttaaagaag ttcaaagagt gtatagactt caaggggttg atgttaatga
     8281 taagcatata gaagttatag taagacaaat gctatctaaa gtaaaagttg aagacccagg
     8341 agatacagat ttattgccag gtggatatga agatgtatta acattcaatg aatgtaataa
     8401 agatgctata gataaaggtc taagaccagc agttgctaaa agagttttac ttggtataac
     8461 taaagcatct cttgcaactg attcattctt atcagcagct tctttccaag aaacaacaag
     8521 agtattaaca gaggcagcaa taaaaggtaa agaagaccac ttaataggac ttaaagaaaa
     8581 tgttatatta ggtaagttaa taccagcagg aacaggaatg aagaaatata gaaatatagc
     8641 tgttgaaaaa attgaagatt aatagcatat agagtaaaga aatcttgaca accattattt
     8701 gtggtgttaa aatagtaaag tgtgttgttt aaaagtacac taagggcttg aacaagctct
     8761 tagtgacttt tagatggcaa taaataagaa ctaaaaagca tcaagctaga aaagtgctaa
     8821 tatcacatat ttatgtgtta tagacattaa attgtaagta acgaatgtta caatcagtca
     8881 gtatgaatat tcatattgat gtagttggcg gtcactgata agatcgaaaa tttggaagga
     8941 ggtgcagatg atgccaacaa ttaaccaatt agttcgtaaa agtagaaaag cgctagagaa
     9001 gaaatctact gctccagcat tacaaaaagg ttacaactct ttaaataaga aagtaactga
     9061 cgctagtgcc ccacaaaaaa gaggggtttg tacttcagta aaaacagtta ctcctaagaa
     9121 gcctaactca gctttaagaa aagttgccag agttagatta actaatggta tagaagtttc
     9181 tgcttatata ccaggagaag gacacaactt acaagagcat agtgttgttc ttataagagg
     9241 aggaagagta aaagaccttc caggggttag ataccatata ctaagaggta ctctagatac
     9301 agcaggtgtt gataagagaa gacaatcaag atctaaatac ggtgcgaaga gacctaaaga
     9361 agctaaaaaa taaaacaatt aatgattgat tatagttcgg tgctttaaat atatatatat
     9421 ttacggcatc acggcattaa atgatttatg tcgagtacct atgatttaag ttaaataatt
     9481 aaggagggaa gcgaaatgcc aagaaaaggt aatattccaa aaagagaagt tttaccagat
     9541 cctatgtatg ggagtaaggt agttactaaa ttaataaata atctaatggt agacggaaaa
     9601 aaaggaaagt ctcaaagaat agtttatgat gctttcgcaa ttgttgctga aaagacagga
     9661 gaagaagctt tagaagtatt taataaagca atggataata taatgcctgt tttagaagta
     9721 aaagcaagaa gagttggtgg agcaaactac caagtgccaa tcgaagttag acctgaaaga
     9781 agacaaacat taggtttaag atggctagta aaatatacta gagctcgtgg agaaaaagga
     9841 atggttgaaa agctagctaa agagataatg gacgcagcta acaacactgg agcttcagtt
     9901 aagaaaaaag aagatactca taaaatggca gaagctaata aagcatttgc tcattacaga
     9961 tggtaggata tatagatatc ttataaattc aaggatatca taaggaggat aaccatggct
    10021 agaaagtttc ctttggaaaa aactaggaat ataggaatca tggctcatat agatgcagga
    10081 aagactacta ctacagaaag aatcctgttt tatactgggc aaactcataa aataggagaa
    10141 actcatgaag gagcttctca aatggactgg atggagcaag aaaaggaaag aggtataaca
    10201 ataacttctg ccgctactac tgcttcatgg aaagaccata gaataaacat aatagatact
    10261 cctggacacg tggacttcac agttgaggtt gaaagatctc taagagttct tgatggttca
    10321 gttgctgtat tctgtgcaaa aggtggggtt gagcctcaat ctgaaaatgt atggagacaa
    10381 gctgaaactt atggagtacc tagaatagct tttataaaca aaatggatat attaggtgca
    10441 gatttctaca atgttataag tatgatgaaa tctagattaa attctaatgc agttcctatg
    10501 caattaccaa taggtaaaga agattcattt ataggtataa tagacttatt aaaaatggat
    10561 gctgttatat acaaagacga cctaggggtt gaaatggaag aaacagatat accagaagat
    10621 atgaaagaat tagctgctga gtggagagaa aaattagttg aatcagtagc tgaaactgat
    10681 gaagaattaa tgatgaaata ccttgaaggt gaagaactta ctatagatga attaaaagtt
    10741 gctataagaa aagctactat agcttgtgaa atgaacccag ttttctgtgg tacagcttat
    10801 agaaacaaag gtgttcagtt agtaatagat gcagttttag attacttacc agcaccaact
    10861 gatataccag ctataaaagg tatattagca gatggagaag aagctgaaag acattcttct
    10921 gatgaagagc cattctctgc attagcattc aaaataatga ctgacccatt cgttggaaaa
    10981 ctagcattct tcagagttta ttctggaacg ttagaatctg gttcttatgt tttaaatgca
    11041 actaagaata agagagaaag aattggtcgt atattacaaa tgcatgccaa tacaagagaa
    11101 gaaataacta aagtttatgc tggagatata gcagcagcag tagggttgaa agatactact
    11161 actggagaca cattatgtga ccctgctaat ccaataatac ttgaatctat ggaatttcct
    11221 gagccagtta tatctgttgc tatagagcca agctctaaag cagctcaaga aaagatgggt
    11281 atagctcttc aaaaattagc tgaagaagac ccaactttca cagttaagac tgaccaggaa
    11341 acaggacaaa ctataatatc tggtatgggt gaattacacc tagagataat agtagataga
    11401 ttattaagag aatttaaagt agaagctaaa gttggtgcac cacaagttgc atatagagag
    11461 actataactc aaccagttga tgttgaatac aagtactcta agcaatcagg tggtagagga
    11521 caatacggtc acgttaagat tagagttgct cctcaagagc caggtgaagg atacaagttc
    11581 acaaacaaaa ctgttggtgg atcagtacca aaagaatatg ttggtccagt tgacatgggt
    11641 atacaaggag ctatgcaatc tggtatagtt gctggatatc cagttgtaga cgttgctgtt
    11701 gagttatatg atggttctta ccatgaggtt gactcttctg agatggcgtt taagatggct
    11761 ggttcaatgg cattcaaaga tgctatgaag aaaggtaatg cagttctttt agagccttac
    11821 ttcaaagtag aagttgttac tccagaagat tacatgggag atgttatggg tgacttaaac
    11881 tcaagaagag gattaatcca aggtatggaa gctagaagtg gtgcacaagt tataaatgca
    11941 ttcgttccac tttcagaaat gtttggatac tcaacagacc taagatcttc aactcaaggt
    12001 cgtgcaactt acacaatgat attcgatcac tatgagcaag ttccagcttc agttgctaag
    12061 aaaatagctg aaggaaaata atttaaaaat aattaataat ataaatataa aaaataatca
    12121 gctagatgag atatatctca tctagcacaa gat
//
LOCUS       DAEPXF010000111        12142 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain HB27
            SAMN13376389-rid12479423.denovo.117, whole genome shotgun sequence.
ACCESSION   DAEPXF010000111 DAEPXF010000000
VERSION     DAEPXF010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13376389
            Sequence Read Archive: SRR10518432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 12142)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12142)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001069624.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001069624.1
            Genome Coverage       :: 315x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/15/2021 01:54:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,165
            CDSs (total)                      :: 4,076
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 89
            rRNAs                             :: 10, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 10 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 19 of 69
            Pseudo Genes (incomplete)         :: 44 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 10 of 69
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12142
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13376389-rid12479423.denovo.117"
                     /strain="HB27"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="China: Hebei"
                     /collection_date="2017"
     gene            <1..324
                     /locus_tag="KRL67_002612"
     CDS             <1..324
                     /locus_tag="KRL67_002612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HBH1523479.1"
                     /translation="AVEKADCYTGNTIESERFFPGWLYDEEDEFVQAAYKGLKEAGID
                     SEITQYSFCTNGSHYAGEAGIRTIGFGPSKENLAHTIDEYIELDQLFKGAEGYYGILK
                     SVYKK"
     gene            340..1923
                     /locus_tag="KRL67_002613"
     CDS             340..1923
                     /locus_tag="KRL67_002613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacylase"
                     /protein_id="HBH1523480.1"
                     /translation="MRTLIKHGLIVDGNKTPAYEGDILIENEKILKISQDLNEEADKV
                     IDAKGRVICPGFIDTHSHSDLVILVNPYNEVKIRQGITTEVLGQDGISMAPLPQEHIS
                     SWRKNLAGLDGESDEIDWKYETTENYLKMMDYNGVGLNETYLVPHGNVRMEAMGLEDR
                     PATKEEIQKMCEITERELKAGAIGLSTGLIYIPCAYSLTEEIIEMCKVVAKYDGVFVV
                     HQRSEADTILTSMEEIIEIGKQSGVKVHFSHFKVCGKTNWKYIPQVIELLEKAEKEGI
                     RVSFDQYPYAAGSTMLGVVLPPWAHSGGTDKLIERLSDENERAKMKKDIANGIEGWDN
                     FIEFAGIDQIFVTSVKTEKNKDTIGKSLLEIGKMRGKDPLDATFDLLKEEENAVGMVD
                     FYGLEEHIIGFMKRDEQNVCTDGLLAGKPHPRAYGSFPKILGRYVRELNVLTIEEAVY
                     KMTKKAATSFSIKDRGELKEGYFADIVIFDKDTVSGCDDFINSMQYPTGIDYVIINGN
                     CVVEEGKYNKIKAGKVLKN"
     gene            1951..3279
                     /gene="ssnA"
                     /locus_tag="KRL67_002614"
     CDS             1951..3279
                     /gene="ssnA"
                     /locus_tag="KRL67_002614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative aminohydrolase SsnA"
                     /protein_id="HBH1523481.1"
                     /translation="MILVGNGRLISQDSLNPYMEDGCVVINDNLIEDIGTTEEMKAKY
                     PVHEFIDAGKKIIMPGLINSHMHIYSSFARGMAVPGKPSENFLEILNNLWWRLDKQLT
                     LEDTKYSAYATYIECIKNGVTTVYDHHASPNAIEGSLFTISDVAKDLGIRTCLCYEVS
                     DRDGGNTIQQGINENVNFIKYAQKDDTDMIKGMFGLHAAFTLSDDTLNKCASEMAGLD
                     TGYHVHVAEGLADLQYNVDKYGKRVLERFNDFGVLKEKTIAVHCIYISTDEISLLNKT
                     NTFVVHNPESNMGNAVGCSPAIELLKRGVTVGLGTDGYTSDMFESLKVANIIHKHHLK
                     DPRVGFEESQTMLFDNNRKIAKAYYKNDLGVLKKGAYADVIVVDYIPHTPLTENTIGG
                     HTIFGMSGRSVDTTIINGKVLMKDRKLINIDEESILAKSRELSQNLWNRI"
     gene            3478..4830
                     /locus_tag="KRL67_002615"
     CDS             3478..4830
                     /locus_tag="KRL67_002615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine permease"
                     /protein_id="HBH1523482.1"
                     /translation="MSQDKSPKIAPVDERLPVSKSWVFSLQHVLAMCAGAVAVPMMIG
                     NAARLDHASIVFLINACLFMAGIGTLIQSVGIGNIVGAKIPVIEGTSFAAVAGMTAIA
                     SSYSDSQVAIQTVFGSVIVAGLFCILLAPVFEKLIRFFPSVVTGTVVMVIGISLFPVG
                     IKWITDNKLEAVEPNSLALALAVLAITLLSFKFLKGIWNSTAILVGIVLGTLLSMAVG
                     MADFSAVNGVGWLNLNVPLKFGMPRFEPSAILSLCLIMLVLMTESVGNMIAIHEMVDK
                     EVTGKNIKRGLFGDGISTALSGIFNSFPITPFGQNTGIVGLTGIKSRFIAVYAGVILL
                     VLGFLPKFAAIIGAIPKPVLGGVGFAMFGMVMVGGVKTLSKVKFDGTKNGVIVAVSVG
                     LSMIPLTNPDFYHNFPTWVQTIFHSGITTGSLSAVLLNIFFNELGSKKEKNNKVEEAK
                     "
     gene            5026..6135
                     /locus_tag="KRL67_002616"
     CDS             5026..6135
                     /locus_tag="KRL67_002616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HBH1523483.1"
                     /translation="MANLKVNLYGMEFLNPIMTAAGPGAKDGALCQEAAKGGAGAICT
                     KTISVLPADVPRPCMAKTNSGFLNTELWSELPKEQWIEEEYKKVKETGLPVIVSMGYS
                     AEQIAEVAPLVKPYADAVELSTHYVGTDVAPIVNALKAAKAVLDVPVFMKMSPHTDIQ
                     TIAKAVEEAGADGLVMINSFGPCMAIDVNTGYPIMGSKAGYGWLSGAPIRPLAIRCIY
                     EASQVVNIPIIGVGGITCGKDVAEMMMAGASAVQVCTEAILKGPSIYGKIAKELNEFL
                     DENGYADVNEIRGLTHKKMAARGFRTHSVAPVVDDNKCIKCKQCVTSCVYEALEVTDK
                     LNIDADKCFGCGLCVTRCPKGALSICYNQVEAMNE"
     gene            6414..7805
                     /gene="hydA"
                     /locus_tag="KRL67_002617"
     CDS             6414..7805
                     /gene="hydA"
                     /locus_tag="KRL67_002617"
                     /EC_number="3.5.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropyrimidinase"
                     /protein_id="HBH1523484.1"
                     /translation="MGIVLKGGKVVTSEKTYCADVRIEDEKIVDIGFDISKENDEIVD
                     VSDSYVIPGGIDTHTHFDLDTGVTKTADNFETGTKAAIVGGTTTILDFATQAKGCSLK
                     DGLEEWHEKAKDKSYSDYGFHMAITDWNDSVCNEMEDMVKEGVSSFKLYMAYKGSLQV
                     DDGVIFEALRKAEEIGGIIGFHCENGDIICELVDKAKAENNLSPKYHQLTRPAILEAE
                     ATSRLMKIAEVANSKVYVVHLSCKESLEEVLKAKQRGVDVIVETCPQYLLLNDELYDL
                     DGFESAKYVMSPPLRNKENNEILWNAISDNQIDTIGTDHCSFNYKGQKELGINDFSKI
                     PNGSPGVEHRMSLLYTYGVCKNKISINKMVEVTSTNAAKLFGMYPNKGTIEIGSDADI
                     LVLNPNKKSKIEVKKQLQNVDYTPYEGYEVDCQIEHVYLRGKEVVRDGELICNTPSGR
                     YINRRLTKGAREW"
     gene            7799..10360
                     /gene="xdh"
                     /locus_tag="KRL67_002618"
     CDS             7799..10360
                     /gene="xdh"
                     /locus_tag="KRL67_002618"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium-dependent xanthine dehydrogenase"
                     /protein_id="HBH1523485.1"
                     /translation="MVEFILNGEKVVAKEGKKLLDFLREDMNITSVKNGCSEGACGTC
                     MVIIDGKATKACVFKTDKLAGKNITTIEGLSERERDVFAYSFASQGAVQCGFCIPGMV
                     ISSKALIDVNNNPTEDDIKKALKGNICRCTGYVKIIKAVDLAAKIFRENIDVPKVNCK
                     GLVGENLPRVDAVGKALGYSEYVDDMRIEGMIYGSAVRAKYPRALVKKIDIEEAKAYP
                     GVVAVLTAKDIPGTIKVGHLKKDWDTLIPEGEITRYLGDGIVLVAAETREALEEAKKL
                     VKIDYEELTPLSTTADALREDAPKIHETGNILVKEHLVRGNAKEKIENSKYVVTKRYT
                     TPATEHAFLEPECAVAGPYEEDGVIIYSTDQGVFATQHECADMLGLPYEKVRVINKMV
                     GGGFGGKEDMSVQHHAALLAYHTKRFVKVQLTRKESIIVHPKRHATEMEFTTACDENG
                     YLTGMKATIISDTGAYASLGGPVLQRLCTHAAGPYNYQDIDITGTAVYTNNPPGGAFR
                     GFGVTQSCFATECNLNLLAEMVGISPWEIRYKNAIRPGQVLPNGQFADEGTAMAETLE
                     AVKEEYEKHPRAGIACAMKNSGVGVGIPDVGRCRLVIKDGKVHIRTSASCIGQGLGTV
                     MTQVVCETASLLPENVVHEVPDTHLTPDSGNTTASRQTVFTGEATKVASNKLKEALDS
                     GKTLLDLEGEEFYGEYESITDKMGCDKENPVSHVAYGYATQVVMLDENGKLEKVVAAH
                     DVGRAINPISLEGQIEGGVVMGLGYALTEDYPVVNSVPTAKFGTLGLFKSTDIPEIQA
                     LIVEKNKDLLAYGAKGVGEICTVPTAPAVQNAYYVFDKEFRTELPLQNTPYSKKK"
     gene            10512..11858
                     /gene="rpoN"
                     /locus_tag="KRL67_002619"
     CDS             10512..11858
                     /gene="rpoN"
                     /locus_tag="KRL67_002619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HBH1523486.1"
                     /translation="MNFNNSLELTQSQKLIMTTQLKQSLSILNMSKLELEEEIKREAE
                     NNPLVEVEKSGEINWEEYIKDMDRSRRLDRTEISYNPDNEVNLENLVKYSSNLYEDLK
                     FQISLYKLTDKELEVCEYIIDSLDEDGYLRTEEKVIVDTLKIDEELFEKCLISVQQLE
                     PSGVGARSLSECLMIQMASLGIYNEILEEIVTKDLNLIANNKYKEISKKYNMSLQKCV
                     ELINIIKTLDPKPGRTCSVEKSVYIQPDVTVEKIDDEFIVYLNEKDSYQIRINSYYKD
                     ILKNSQSDESAKEFIKERLNSATGLMKNIESRKTTVLKIAEEIVKAQDEFLRKGTKYI
                     KPLKMKDIAEKLEFHESTISRGVNGKYMLTPFGVYEFRYFFSSAIETENNEMASSTSI
                     KKIIKETIKDENKKKPLSDDHISKLLKEKGINVARRTVAKYREELGIPSSSKRKEY"
BASE COUNT         4834 a         1417 c         2471 g         3420 t
ORIGIN      
        1 gcagttgaaa aagctgattg ctatacagga aatactatag aaagtgaaag attcttccca
       61 ggttggttat atgacgaaga agatgaattt gttcaagcag catataaggg actaaaagaa
      121 gctggaatag attcagaaat aactcaatac tcattctgta cgaatggaag tcactatgca
      181 ggtgaggctg gaataagaac tataggattt ggtccttcta aggaaaatct tgctcataca
      241 atagatgagt atatagaatt agaccaatta tttaaaggtg cagaaggata ttatggtata
      301 ttaaaatcag tttataaaaa ataattagga gatggttttg tgagaacttt aataaaacat
      361 gggcttatag tagatggaaa taaaacacca gcatatgaag gagatatttt aatagaaaac
      421 gagaaaatat taaaaatatc acaggattta aacgaagaag ctgacaaagt tatagatgca
      481 aagggtagag ttatatgtcc aggtttcata gatactcata gccattcaga cctagttatt
      541 ttagttaatc catataatga ggtaaagatt agacagggaa ttacaacaga agtgttgggc
      601 caagatggta tatctatggc ccctctacct caagagcata tatcttcttg gagaaaaaat
      661 ttagcaggtc tagatggtga aagtgatgaa attgattgga aatatgaaac aacagagaat
      721 tatttaaaaa tgatggatta caatggggta ggtttaaatg aaacttattt agtacctcat
      781 ggaaacgtta gaatggaagc aatgggactt gaagatagac ctgctactaa agaagaaatt
      841 caaaaaatgt gtgaaatcac agagagagaa ttaaaagcag gagctatagg attgtctact
      901 ggacttatat atataccttg tgcatactca ttgactgaag aaattataga gatgtgtaag
      961 gtagtagcaa aatacgacgg agtatttgta gttcaccaaa gaagtgaagc agatacaatt
     1021 ttaacatcaa tggaagaaat catagaaatt ggaaaacaat caggagtaaa agttcatttt
     1081 tcacatttta aggtttgtgg taaaacaaac tggaagtata taccacaagt tatagagctt
     1141 ttagaaaaag cagaaaagga aggcataaga gtatcttttg accaatatcc atatgcagca
     1201 ggaagtacaa tgcttggagt agttttacct ccttgggcac actctggagg aactgataag
     1261 ttaatagaaa gattaagtga tgaaaacgaa agagcaaaaa tgaaaaaaga tatagcaaat
     1321 ggaatagaag gatgggataa ttttatagaa tttgcgggaa tagaccaaat atttgtaaca
     1381 agtgtaaaaa cagaaaagaa taaagataca attggtaaga gcttattaga aattggaaaa
     1441 atgagaggaa aagacccatt agatgcaacg tttgatttat taaaagaaga agaaaatgca
     1501 gttggaatgg tagatttcta tggattagaa gaacatataa taggatttat gaaaagagac
     1561 gagcaaaatg tgtgtacaga tggtctttta gcaggtaaac cacatccaag agcttatggt
     1621 tctttcccta aaatactagg aagatatgtt agagagttaa atgtactaac aatcgaagaa
     1681 gctgtttata aaatgactaa aaaagcagct acaagcttta gtataaaaga tagaggcgaa
     1741 ttaaaagaag gatattttgc agatatagtt atatttgaca aggatacagt tagtggttgt
     1801 gatgatttta tcaactctat gcagtaccct acaggtatag attatgtaat tataaatgga
     1861 aattgtgttg ttgaagaagg aaaatacaat aaaattaaag caggaaaagt acttaaaaat
     1921 taaatcttag ctttaaggag gaataaattt atgattttag tggggaatgg gaggctaata
     1981 agccaagatt ctttaaatcc ctatatggaa gatggatgtg tagtaataaa tgataatcta
     2041 attgaagata taggtactac agaagaaatg aaagctaaat atccagtaca tgaatttata
     2101 gatgcaggca agaaaataat catgcctggt ctaataaatt cacatatgca tatatacagc
     2161 tcatttgcta gaggaatggc agttccagga aaaccttctg agaatttttt agagatacta
     2221 aacaatttat ggtggagact tgacaaacag cttacactgg aagatacaaa atacagtgca
     2281 tatgcaacgt atatagaatg tataaaaaat ggtgtaacaa cagtttatga ccatcatgca
     2341 agtccaaatg caattgaagg cagtttgttt acaatatcag atgttgcaaa ggatttggga
     2401 attagaacgt gtctttgtta tgaagtgtct gatagagatg gcggaaatac aatccaacaa
     2461 ggtatcaatg aaaacgtaaa ctttataaaa tacgctcaaa aagatgatac agacatgata
     2521 aaaggaatgt ttggacttca tgctgcattt acactatctg atgatacatt aaataaatgt
     2581 gcttcggaga tggctggtct tgatacaggt taccatgtac atgttgctga aggactagca
     2641 gacttacaat ataatgtaga taaatatgga aaaagggttt tagaaagatt caatgatttt
     2701 ggagtactaa aagaaaaaac aatagcagtt cattgtatat atataagtac agatgaaatc
     2761 agccttttaa ataaaacaaa tacatttgta gttcataatc cagaatccaa tatgggaaat
     2821 gcagtgggat gttctcctgc aatagagcta ttaaaaaggg gagttacagt aggattagga
     2881 actgatggat atactagtga tatgtttgaa tcattaaaag ttgccaatat aattcacaaa
     2941 catcacttaa aagacccaag agttggattt gaagaatcac aaacaatgtt atttgataac
     3001 aatagaaaaa tagctaaagc ttattataaa aatgacctag gagtacttaa aaaaggtgct
     3061 tatgcagatg ttatagtagt tgattatata ccacatactc cattgacaga aaacacaata
     3121 gggggacaca ctatttttgg aatgagtgga agaagcgtag atacaactat aataaatgga
     3181 aaagtactta tgaaagatag aaagttaata aacatagatg aagaaagtat tctagcaaaa
     3241 agtagagaat taagtcaaaa tctatggaat agaatttaaa taattgatta tgcaaaattc
     3301 tatgaagcag gaaccctgtg actttagtca tgggaggttc agattaatat acaagtaaga
     3361 aacaagaatt tagatgttaa aagcagagat taaacaaaaa taggaattta aatgaagtat
     3421 ataaataaaa aatatacgga atagaatatt aaattaaata tagaagggag acccaaaatg
     3481 tctcaagata aaagccctaa aatagctcca gtagatgaaa ggttacctgt atccaaatct
     3541 tgggtatttt cactacagca cgttttagca atgtgtgcag gagcagtagc agttcctatg
     3601 atgataggaa atgctgcacg actagatcat gctagtattg tttttttaat aaatgcttgc
     3661 ttgtttatgg caggtatagg aacactcata caaagtgttg gtataggaaa tattgtaggt
     3721 gctaaaatac cagtaataga aggaactagt tttgccgctg tggcaggtat gacagcaata
     3781 gcctcatcat atagtgattc acaagttgca atacagacag tatttggttc tgtaatagta
     3841 gctggattgt tttgtatatt acttgctcca gtatttgaaa aattaataag atttttccct
     3901 agtgtagtta ctggaacagt tgttatggta ataggtattt cactatttcc agttgggata
     3961 aaatggataa ctgacaacaa attggaagcg gttgaaccaa actcattagc attagcttta
     4021 gcagtattgg caataacttt actttcattt aaattcttaa aaggaatttg gaatagcaca
     4081 gctatattgg taggtatagt acttggtaca ctactttcca tggcagttgg aatggcagat
     4141 tttagtgcag tgaatggagt tggttggctt aacttaaatg tacctttaaa atttggaatg
     4201 cctagatttg aaccatctgc aatactatca ttgtgtttga ttatgttggt acttatgacg
     4261 gaatcagttg gtaatatgat agctattcat gaaatggtag acaaagaagt aacaggtaaa
     4321 aatattaaaa gaggtctatt tggagatgga atatctacag cattatctgg aatattcaat
     4381 tcatttccaa taactccatt cggtcaaaat acaggaatag taggtcttac aggtataaaa
     4441 tcaagattta tagcagttta cgcaggggtt atattgctag tacttggatt cttaccaaaa
     4501 tttgcagcaa taataggagc aatacctaag cctgttcttg gaggcgtagg atttgcaatg
     4561 tttggaatgg taatggttgg aggagtaaaa actttaagca aagtgaaatt tgatggaact
     4621 aaaaatgggg ttatagtagc agttagtgtt ggtttgtcta tgataccact aacaaatcct
     4681 gatttctatc ataatttccc aacttgggta caaacaatat tccatagtgg aataacaaca
     4741 ggtagtttat cagcagtgct tttaaacata ttcttcaatg aactaggttc taaaaaagaa
     4801 aaaaataata aagttgaaga agcaaagtaa aaataaaaca agtaagtaaa aatctatatt
     4861 gattaaaagt tattaaacat taataaaaat aattcagaga tattaataat tcaacaaaac
     4921 tatcttatat tttaaataat ataaaaaagt aaataaataa cgaacacaaa taaaagaata
     4981 caatctaaat attaaaaata tttaatttaa gggagagata ataatatggc taatttaaaa
     5041 gttaatttat atggaatgga atttttaaac ccaattatga cagcggcagg tcctggagca
     5101 aaagatggtg ctctctgtca agaggcagca aaaggtggag caggagctat atgtacaaaa
     5161 actatatccg tattaccagc tgatgtacca agaccatgta tggcaaaaac taatagtggt
     5221 tttttaaaca cagaattatg gtcagagctt ccaaaagagc aatggataga ggaagaatac
     5281 aaaaaggtaa aagaaacagg tcttccagta atagtaagta tgggatattc agcagagcaa
     5341 atagctgagg ttgctccttt agtaaaacct tatgcagatg cagttgaatt atctactcat
     5401 tatgtaggta cagatgttgc accaatagta aatgctttaa aagctgcaaa agctgtttta
     5461 gatgtaccag tatttatgaa aatgagtcct catactgata ttcaaactat tgcaaaagct
     5521 gttgaagaag caggtgcaga tgggcttgta atgataaact cattcggacc atgtatggca
     5581 atagatgtaa atacaggata ccctataatg ggaagtaaag ctggatacgg atggttatca
     5641 ggtgctccaa taagacctct agcaataaga tgtatatacg aagcttctca agttgtaaat
     5701 ataccaataa tcggtgttgg tggaatcact tgtggaaaag acgtagctga aatgatgatg
     5761 gctggagcaa gtgctgttca agtatgtaca gaagcaatcc ttaaaggacc ttctatctat
     5821 gggaaaattg ctaaagaatt aaatgaattt ttagatgaga atggatatgc tgatgttaat
     5881 gaaataagag gattaactca taagaaaatg gcagctagag gatttagaac tcattcagta
     5941 gctccagtag tggatgataa caagtgcatc aaatgtaagc aatgtgtaac aagctgtgta
     6001 tacgaagctt tagaagttac agataagttg aacatagatg cagacaagtg ttttggatgt
     6061 ggattatgtg taactagatg tccaaaagga gctttaagca tctgctacaa tcaagtagaa
     6121 gctatgaatg agtaataatt atatagaatt ttggtaatta acttagagta ataggtatta
     6181 ggataataaa atatatcaga taaacacgca ttatgcatta ataatacagt ctaaagttaa
     6241 taattttatg atattgattt aactaaatat tagtttagct gaatattaat ttaattaggt
     6301 attggtttaa tcttaatata gatttactac gtataagtga actaactaga ttactttaag
     6361 ttaatttttt catataaaat aaattgatac attacaagta aggtggagtc tatatgggaa
     6421 ttgttttaaa aggtgggaaa gtagtaacaa gtgaaaaaac ttactgtgca gatgtaagaa
     6481 tagaagatga aaaaatagta gatataggtt ttgatatatc aaaagaaaat gatgaaatag
     6541 ttgatgttag tgattcttat gttataccag gaggtataga tacacacaca cactttgact
     6601 tagatacagg cgttactaag acagcagata actttgagac aggaacaaaa gctgctatag
     6661 taggaggaac tactactata ttggattttg ctacacaagc taaaggatgt tctttgaaag
     6721 acggattaga agaatggcat gaaaaagcta aggataaatc ctattcagat tatggctttc
     6781 atatggctat aacagattgg aatgactcgg tttgtaatga aatggaggat atggtgaaag
     6841 agggagtatc ttcatttaaa ctgtatatgg cgtataaagg aagccttcaa gttgatgatg
     6901 gtgtcatatt tgaagcattg agaaaagcag aagagatagg tgggattatt ggtttccatt
     6961 gtgaaaatgg ggatataata tgtgaacttg tggataaagc aaaggcagaa aataatttat
     7021 ctccaaaata tcatcaatta actagaccag ctatactgga ggcagaagct acgtcaagac
     7081 ttatgaagat agctgaagtt gcaaactcta aagtatatgt agttcactta agttgtaaag
     7141 aatccttaga ggaggtttta aaagctaagc aaagaggtgt ggatgtaata gttgaaactt
     7201 gtcctcaata ccttctatta aatgatgaat tatatgattt agatggattt gaaagtgcta
     7261 agtatgtaat gtcaccacct ttaagaaata aagaaaataa tgaaatatta tggaatgcta
     7321 tttcagataa tcaaatagac actataggaa cagaccattg ttcttttaat tataagggac
     7381 agaaggaact tggaataaac gatttcagta agataccaaa tggttctcca ggtgtagaac
     7441 ataggatgag tcttttatac acatatggag tctgtaaaaa taaaatatct atcaacaaga
     7501 tggtagaagt aacatcaact aatgcagcaa agttgtttgg aatgtatcct aataaaggaa
     7561 ctatagaaat aggcagtgat gcagatatat tagtcttaaa tccaaacaaa aaatccaaaa
     7621 tagaagtgaa aaaacaatta caaaatgtag attatacacc ttatgaaggg tatgaagtgg
     7681 attgtcaaat agaacatgtt tatttaagag gaaaagaagt agttagagat ggagaattaa
     7741 tctgtaatac tccaagtggg agatatataa ataggcgtct aacaaagggg gctagagaat
     7801 ggtagaattt atattaaatg gagagaaagt tgttgcaaaa gaaggcaaaa aacttttaga
     7861 cttccttaga gaagatatga atataacttc agttaaaaat ggatgttcag aaggtgcttg
     7921 tggaacatgt atggttataa tagatggtaa agctactaaa gcatgtgtat ttaaaactga
     7981 taaattagct gggaaaaaca taacaactat agagggatta tcagagagag aaagagatgt
     8041 ttttgcatat tcttttgcta gtcaaggagc tgtacaatgt ggattttgta ttccaggtat
     8101 ggtaattagt tcaaaagcac ttatagacgt taataataat cctactgaag atgatataaa
     8161 gaaagcttta aaaggtaata tatgtagatg tacagggtat gtaaaaataa ttaaagctgt
     8221 cgatttagca gcaaagattt ttagagaaaa tatagatgtg ccaaaagtaa actgtaaagg
     8281 tctagtaggt gaaaatcttc ctagagttga tgcagttggc aaagcacttg gatattctga
     8341 atatgtagat gatatgagaa ttgaaggtat gatttatggt tcagcagtta gagctaagta
     8401 tcctagagct ttagttaaaa aaatagatat agaagaagca aaagcttatc caggagttgt
     8461 tgcagttcta acagctaaag atattccagg aactataaag gtagggcact taaagaagga
     8521 ttgggatact ttaataccag aaggtgaaat aactagatac ttaggagatg gaatagtttt
     8581 agtagcagca gaaactagag aagcgttaga agaggcaaaa aagctagtta agatagatta
     8641 tgaggaatta acaccattaa gtactacagc agatgcactt agagaagatg ctccaaagat
     8701 acacgagaca ggtaatatac ttgttaagga acatttagta agaggaaacg ctaaagaaaa
     8761 gatagaaaat tcaaaatatg ttgtaactaa aagatatact acacctgcta cagaacacgc
     8821 atttttagag cctgaatgtg cagtagctgg tccttatgaa gaggatggag taataatcta
     8881 ttcaacagac caaggggtgt ttgccactca acatgaatgt gcagatatgt tagggctacc
     8941 atatgagaag gtaagagtta taaacaaaat ggtaggagga ggatttggtg gaaaagaaga
     9001 catgagtgtt caacaccatg cagctttact tgcataccat actaagagat ttgtaaaagt
     9061 acagttaaca agaaaagaaa gtattattgt tcatccaaaa cgacatgcca cagaaatgga
     9121 atttacgaca gcatgtgatg aaaatggata cttaacaggt atgaaggcaa cgataatatc
     9181 tgacacaggt gcatatgcct ctcttggagg accagtacta caaagactgt gtactcatgc
     9241 agcaggacca tacaattatc aagatattga tataacagga acagcagtat atactaataa
     9301 ccctcctgga ggagcattta gaggatttgg agttacacag agttgttttg caactgaatg
     9361 taatctaaat ttacttgctg aaatggttgg aatatctcct tgggagataa gatataaaaa
     9421 tgcaataaga cctggtcaag ttttaccaaa tggtcaattt gcagatgaag gaacagcaat
     9481 ggcagagaca ctagaagctg taaaagaaga gtatgaaaag catccacgtg caggtatagc
     9541 ttgtgctatg aaaaatagtg gggttggagt tggtatacct gatgttggaa gatgtagatt
     9601 ggtgataaaa gatggcaaag ttcacataag aacaagcgct tcttgtatag gtcaagggct
     9661 tggtactgtt atgactcaag tagtttgtga aactgctagc ctacttcctg aaaatgtagt
     9721 gcatgaagtt ccagatactc atttaacacc agactctggt aatactacgg cttcaagaca
     9781 gacagtattt acgggagaag ctactaaggt tgcgtcaaat aagttaaaag aagctttaga
     9841 ctctggcaaa actttacttg atttagaggg agaagagttt tatggagaat atgaaagtat
     9901 tacagataaa atgggttgtg acaaagaaaa tccagtaagt catgttgctt atggatatgc
     9961 aacacaagtt gtaatgctgg atgaaaatgg aaaattggaa aaagtagtag cagcacatga
    10021 tgttggaaga gctataaatc caatcagttt agagggtcaa atagaaggtg gagtagttat
    10081 gggtcttgga tatgctctaa ccgaagatta cccagtagtt aactctgttc ctacagctaa
    10141 gtttggtaca ttaggtttat ttaaatctac agatatacca gaaatacaag ctttaatagt
    10201 tgaaaagaac aaagatttat tggcttatgg ggcaaaaggt gtaggagaaa tttgtacagt
    10261 tcctactgca ccagcagtac aaaatgccta ttatgtattt gataaagaat ttagaactga
    10321 actaccacta caaaatactc catatagtaa aaaaaagtaa caaaaaaaag aaatattaaa
    10381 taaaaaaata gatgaaattg taaataaaaa aaaccctttt gcataaggta tattttaaat
    10441 gatagaatat acatatacag gagggttttt cttttataaa ataagtttaa taaaattgag
    10501 aggtgagcag aatgaatttt aataatagtc tagaattaac tcagtctcag aagctaataa
    10561 tgactacaca acttaagcaa tcattatcaa tattaaatat gagtaagtta gagttagaag
    10621 aagagataaa aagagaagca gagaataacc cattagttga agtcgagaaa agtggggaga
    10681 taaattggga agagtatata aaagacatgg ataggtcaag acgtttagat agaacagaga
    10741 taagctataa tcctgataat gaagttaatc tagaaaattt agttaaatat tcgtcaaatt
    10801 tatatgaaga tttaaaattt cagataagcc tgtataaact tacagacaaa gaattggaag
    10861 tctgtgagta tataattgat agtctagatg aggatggata tttaagaact gaggaaaaag
    10921 taattgttga taccttaaag atagatgaag aactttttga aaaatgttta attagtgttc
    10981 agcagttgga acctagtgga gtaggggcaa gaagtctttc agaatgtctg atgatacaaa
    11041 tggctagttt aggaatatat aatgaaattt tagaggaaat cgttacaaaa gacttaaatt
    11101 taattgcaaa taataaatac aaagagataa gtaaaaagta caatatgtca ttacaaaaat
    11161 gtgttgaact aataaacata attaagacat tagacccaaa accaggaaga acttgttcag
    11221 tagagaaaag tgtatacatt caacctgatg taacagtaga aaaaattgat gatgaattta
    11281 ttgtatattt aaatgaaaaa gattcttatc aaataagaat caatagttat tataaagaca
    11341 tattaaaaaa ttcacagtct gatgaaagtg caaaagaatt tataaaagaa agactaaatt
    11401 cagcaacagg acttatgaaa aatatagaga gtaggaaaac tacagtactt aagattgcag
    11461 aagagatagt aaaagctcaa gatgaatttt tgagaaaagg gacaaaatat ataaaacctt
    11521 tgaaaatgaa agacatagca gaaaaattag aatttcatga atctactata agtagaggtg
    11581 taaatggtaa atatatgcta acaccttttg gcgtatatga atttaggtac tttttcagta
    11641 gcgccattga gactgagaat aatgaaatgg catctagtac aagcataaaa aaaattataa
    11701 aggaaacaat aaaagacgaa aataaaaaga aacctttaag tgatgaccat atatctaagt
    11761 tactaaaaga aaaaggaatc aatgttgcaa gaagaacagt tgccaaatat cgtgaagaat
    11821 taggaatccc atcttcaagt aagagaaaag aatactaata agtaaaagac aaatatcagt
    11881 attgtaaggg gaaaaggcta attaaaagat gaatgtggat taggctaaaa attttatgaa
    11941 ataaaataat agaatatgga aaagtattaa aatgctataa gatattaaaa aactggggaa
    12001 cattaaaaca ttaaaatagc aaagaacata aaaaactaaa gaagattaaa acattaaaga
    12061 acattagagc tttgaggaat attagaactt taaagaatat taaaatttat aaaatataaa
    12121 aatactaaaa agaattgaaa aa
//
LOCUS       DAEPXF010000112        12083 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain HB27
            SAMN13376389-rid12479423.denovo.118, whole genome shotgun sequence.
ACCESSION   DAEPXF010000112 DAEPXF010000000
VERSION     DAEPXF010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13376389
            Sequence Read Archive: SRR10518432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 12083)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12083)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001069624.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001069624.1
            Genome Coverage       :: 315x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/15/2021 01:54:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,165
            CDSs (total)                      :: 4,076
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 89
            rRNAs                             :: 10, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 10 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 19 of 69
            Pseudo Genes (incomplete)         :: 44 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 10 of 69
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12083
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13376389-rid12479423.denovo.118"
                     /strain="HB27"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="China: Hebei"
                     /collection_date="2017"
     gene            92..1156
                     /locus_tag="KRL67_002620"
     CDS             92..1156
                     /locus_tag="KRL67_002620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HBH1523487.1"
                     /translation="MKRVDERNTMFARANYKKDSTAYNDYYKKNPDKKEIDDSIRNRP
                     NLCSEGTMTYNELNSPMASSAFDFLSDIKSLCEGKVSDTKVDVDAKSMTKKIKGLAKQ
                     YGASVVGITKLKDYHIYTNRGRHEENYGEEVNLTHKYAIVFGCEMDKEMINRAPMICE
                     VIETSKCYVDASIVGMILSYYIRNLGYDARNHMDANYLVMPVFIARDAGLGDIGRNAI
                     LTNKDYGSRLRLGVVTTDIPLLEDEYVDFGLEDFCKVCKKCSFNCPSHSLSNDIKISD
                     DGKYNWVIEHETCYIKWRYLGTDCGMCISACPFSQNLETIKNTTSFKANNELIQKALD
                     EYTSKFGKRIFIPGNPSWLK"
     gene            1314..2000
                     /locus_tag="KRL67_002621"
     CDS             1314..2000
                     /locus_tag="KRL67_002621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBH1523488.1"
                     /translation="MEKILVVEDDSILNKTLSYNLIEDGYIITSKFTAKSALKSIFEC
                     EFDLIILDINLPDKSGFELCNEIKENYNIPIIFLTANDMECDMIKGYELGALDYITKP
                     FNINVFKQKVKAFLNHFTTKTKQDYYRDGYLEINFSELSANINGNQIIFTPLEYRTLK
                     LLTENPKNILTRKVLLEKLWDIDANFVDEHTLTSVISRIRSKIEKDNLQYIKTVYGMG
                     YMWLGERNEF"
     gene            1990..3021
                     /locus_tag="KRL67_002622"
     CDS             1990..3021
                     /locus_tag="KRL67_002622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBH1523489.1"
                     /translation="MNFKKFSFNSIYLIIVVGIIFTVILFNISIYIITDDLKLVALLL
                     IYTIVLCGFIALIIFLLHKKIVIFCSKICHTLDSMMNSEYNIEIELEEETILSRINHR
                     LIRMYEVIQESRNSIAIEKADLQELVSDISHQIKTPIANLKMINTTLLNQTFKTEIQN
                     DFLMDMENQLDKLDFLMQSMVKTSRLETGIITLSKKKNSIYETIATALSGILFDAEKK
                     NIEVTVDCDPNLYIIHDKKWTSEAIFNILDNAVKYTSSNGKIRVATECWVMYTKIDII
                     DNGKGISESHQAEIFKRFYREEDVHDIEGIGIGLYLSRKIISLQGGYIKVTSEIGKGS
                     TFSIFLPNK"
     gene            3103..3768
                     /locus_tag="KRL67_002623"
     CDS             3103..3768
                     /locus_tag="KRL67_002623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBH1523490.1"
                     /translation="MSILQVINLKKYYGQNSNITKALDSVTLSIEKGEFISIVGTSGS
                     GKSTLLNMMGGLDNPTSGKVIIKGKELSTMTDEQLTIFRRRNIGFIFQNYNLVPLLNV
                     YENIILPIGLDGETIDKDFINEIINMLSLEEKLYDMPSNLSGGQQQRVAIARALVTKP
                     AIILADEPTGNLDSRTSSDVLSLLKVTSKKFNQTIVMITHNNEIAQLTDRIIRIEDGK
                     IVN"
     gene            3781..6197
                     /locus_tag="KRL67_002624"
                     /pseudo
     CDS             3781..6197
                     /locus_tag="KRL67_002624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861404.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
     gene            complement(6375..7166)
                     /locus_tag="KRL67_002625"
     CDS             complement(6375..7166)
                     /locus_tag="KRL67_002625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HBH1523491.1"
                     /translation="METKQYLIDFYNTYDEDSRLALKHGMVEFLTTMHYIDKYIKSGD
                     CVLEIGAATGRYSHTLARQGYDVDAVELVEHNIGVFRRNTQSNENISITQGNAMDLSI
                     FPDNKYDITLLLGPLYHLYNKEDKQQALHEAIRVTKPGGVVFAAYVISDGCLIDEGFH
                     RGNINVSEYIEKGLIDPQTFAAKSEPKDLFELVRKENIDDLMSVFNVTRLHYVASDGL
                     ALYMREAVDSMDDDAFALYLKYHLTTCEREDLVGVTSHAIDIFRK"
     gene            complement(7199..7786)
                     /locus_tag="KRL67_002626"
     CDS             complement(7199..7786)
                     /locus_tag="KRL67_002626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBH1523492.1"
                     /translation="MPPKVKITKEMILNTVLDITREVGFEAVNARSIASKLQCSTRPI
                     FTCYENMNELKNEFLAFAYEYYNQYVVNYRNSRSVSPYLLLPLSYIEFAQEETHLFKL
                     LFINDLDLMMEEANDFYKEIDNEKKARLFSESIGIELECAKVIFLDLFLYTHGMAVLT
                     ATKKLTLDRYSAEKMLKNILTAFIRQEKPDWNLSI"
     gene            complement(7964..9355)
                     /locus_tag="KRL67_002627"
     CDS             complement(7964..9355)
                     /locus_tag="KRL67_002627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="benzoate/H(+) symporter BenE family transporter"
                     /protein_id="HBH1523493.1"
                     /translation="MERLARREQPHIKFGMFKIRIPFIHYRFEKPEAIQGIISSMTSL
                     GTIGLSTQILGLPYEVAWSMAIINSILYCLHVFMGDPVVPGWITASITLTTAYLLKFS
                     MGIERIQALTALQIDLGIIFILMGITGVAGKLVSKIPNSIKGGILMGVGISTIISEFN
                     PKGRFDSYPISITVGILVACFVMFSERFDTLKVKNKFLFHLGEYGVVSAILVSLIVGI
                     FSKEIEIPRFSFDNLVYIVDFKNLINTVSPFGIGFPSVMLFIQGIPMAFMIYIIAFGD
                     FITGENLVLSESENRKDEYIDFNSNRSNVISGIRNIFMAIISPYIPMCGPLAATLTGS
                     VAQRYKVGKEAMQSVFSGMGTLVWVSAIFICFYPIAQIATPLIPLALSVTLLVQGYLC
                     TKLSMELCDTGIEKGLIGLMGGVIAAKGGTWGLAVGFILYFVLIDSKKRKEKDINSIE
                     DCVYEILDKKKSI"
     gene            complement(9522..10820)
                     /locus_tag="KRL67_002628"
     CDS             complement(9522..10820)
                     /locus_tag="KRL67_002628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase/BAAT N-terminal
                     domain-containing protein"
                     /protein_id="HBH1523494.1"
                     /translation="MKVNVYNVESFVDEKLKIMVSDLIPNTKLKISVKMEFPWCKGEE
                     FSSYGVFCSNEKGEIDLDLVEPIEGTYKADNCMGLIYSLEKSKTEGKNFAENISIDKP
                     IIMNMIFETSTERKEVELKRIFKSEDITTKYISDEFIGKLFYKQNSNNKTILMLGGSD
                     GNLDALDLLAAPLASRGFNVLTVAYFALEGLPDKLEEVPLEYFEKVFEWISENEITST
                     KEIFVHGTSKGGELAFLLASIYEQIKKIVVSQPHTYCFQALNGLMSGNDTSSWSYKGE
                     PFPYIKVDNDIFFEEQKKNISKGIPFGFNSTYKKSLKRAENRKEARIKIENSKADILM
                     IAGKEDNIWNSYEACLETLEILEENNYPYNVELLTYNNMGHPLPIPYIMPLKETLCMS
                     MSGGVFSSGGTVEGNSKGQFESWNRTIEFYKKPLSSDLNN"
     gene            complement(11181..12056)
                     /locus_tag="KRL67_002629"
     CDS             complement(11181..12056)
                     /locus_tag="KRL67_002629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase"
                     /protein_id="HBH1523495.1"
                     /translation="MITFDVNGAIIRFDDTKNNYNKIRKIFKLYALEISKNFERDCLN
                     SFQNLKQISDRGLSLGEKYIEESFKKGIETIVSFGIITIDIDTFKSVYCEKYLDFKRL
                     FNNLNKTFIIPNKNKKNGHASFLEIESTIKKMSNYLYNDCFKIHYAVVDALIENRITA
                     VHSYIDEESIKKSNALFNNYKDGFIGKADECKVVKQIITLNPYREDVYEFLVKEDGDF
                     SKEIERLTEFLGYDIREYKASLMDIYINELMEIDENDVENAKEKIEKYARYIGCSDEN
                     LYVARVNAIYTFANA"
BASE COUNT         4415 a         1729 c         1547 g         4392 t
ORIGIN      
        1 aaattgaaaa tataacgtta ttcttatata attaaattat acaacaataa agttatattt
       61 tttaaatata tttttactag ggggaataga attgaaaagg gttgatgaga gaaatactat
      121 gtttgcaaga gcaaattaca aaaaagatag tactgcttat aatgactatt ataagaaaaa
      181 tcctgacaaa aaagaaattg atgatagtat aagaaataga ccaaatttat gtagtgaagg
      241 aacaatgact tacaatgagt taaactctcc aatggcaagt agtgcattcg attttttatc
      301 tgatatcaaa tctttatgtg aaggaaaagt ttctgatact aaagtagatg tagatgcaaa
      361 gtcaatgaca aaaaaaataa agggtcttgc aaagcagtat ggagcttctg tagttggtat
      421 aactaaatta aaagactatc atatttatac aaataggggt agacatgaag aaaattacgg
      481 tgaagaagtt aatctaactc ataagtatgc aattgtattt ggatgtgaga tggataaaga
      541 aatgataaat agagctccaa tgatatgtga agttatagaa acatcaaagt gctatgtgga
      601 tgcatctata gtcggcatga ttttaagtta ctatataaga aatttaggat atgatgctag
      661 aaatcatatg gatgctaatt atttagtaat gcctgttttt atagctcgtg atgctggttt
      721 aggagatata ggaagaaatg ctatacttac aaataaagat tatggctcta gacttagatt
      781 aggtgttgtt actacagata ttcctctttt agaagatgag tatgttgatt ttggactaga
      841 agatttttgt aaggtctgta aaaaatgctc ttttaactgc ccttctcatt cgctttctaa
      901 tgatattaaa attagtgatg atggaaaata taattgggta attgaacatg agacatgcta
      961 tattaaatgg agatatttag gtacagattg tggtatgtgt atatctgctt gcccatttag
     1021 ccaaaacctc gaaactataa agaatactac ttcatttaag gctaataatg agcttattca
     1081 aaaagcttta gatgagtata cttcaaaatt tggtaaaaga atatttatac ctggaaatcc
     1141 atcttggttg aaataatttt atttaataaa gaatctgtcc tagaatttgt cataggacgg
     1201 gttctttatt aaatatataa acttttaaat actttttctc gtttataaca attcagcaat
     1261 atttttagtc tataattgac attataaaat attaaactaa aaggagtaac tgtatggaaa
     1321 aaatattagt agtggaagat gattctatat taaataaaac tctatcatat aacctaattg
     1381 aagatggata tataattact tcaaaattta cagctaaatc tgcactaaag tctatttttg
     1441 aatgtgaatt tgatttaata attttggata ttaatttgcc agataaaagt gggtttgaat
     1501 tatgtaatga aataaaggaa aattacaata taccaataat ttttttgact gctaatgata
     1561 tggaatgtga tatgattaaa ggctatgaac ttggagcttt ggattatata acaaaaccat
     1621 ttaatatcaa tgtatttaaa cagaaagtta aagctttttt aaatcatttt acaacaaaaa
     1681 caaaacaaga ttactatagg gatggatatc ttgaaattaa cttttctgag ttatctgcca
     1741 atataaatgg aaatcaaatt atatttacac cattagaata tcgtacttta aaactgctta
     1801 ctgaaaatcc taaaaatatt ttaacaagga aagtactttt agaaaaactc tgggatatag
     1861 atgctaactt tgtagatgaa catactttaa catctgttat aagtaggatt agaagcaaaa
     1921 ttgaaaaaga caatcttcag tatattaaaa cggtatatgg tatgggatat atgtggttag
     1981 gtgaaagaaa tgaattttaa aaaattttct tttaattcta tttatttaat aattgttgtt
     2041 ggtataattt ttacagttat tttatttaat atctcaattt atataataac tgatgattta
     2101 aaattagttg ctctattatt aatatataca atagtacttt gtggatttat agcattaata
     2161 atatttttac ttcataaaaa aatagttata ttttgttcta aaatatgtca cactttagat
     2221 agtatgatga atagtgaata caatattgaa attgaattag aagaagaaac tattttatca
     2281 cgtatcaatc acagattaat tagaatgtat gaagttattc aagaaagtcg caatagtatt
     2341 gctatagaga aagctgattt acaagaacta gtttctgata tatctcatca aattaaaact
     2401 cctattgcaa atttaaaaat gattaatact actctactaa atcaaacatt taaaacagaa
     2461 attcaaaatg attttttaat ggatatggaa aaccaattag ataaattaga ttttttaatg
     2521 caatctatgg taaaaacctc cagactagag acaggtataa tcacactatc taaaaagaaa
     2581 aattcaattt atgaaacaat tgctacagca cttagtggaa ttttgtttga tgctgaaaaa
     2641 aagaatattg aagttacagt tgattgtgac ccaaatttat atataattca tgacaagaaa
     2701 tggacttctg aagctatttt taatatatta gataatgctg taaaatacac aagttctaat
     2761 ggtaagatac gtgtagctac agaatgttgg gttatgtata caaaaataga tattattgat
     2821 aatggaaaag gaatatcaga aagtcatcaa gcagaaatct ttaaaagatt ttatcgtgaa
     2881 gaagatgtac atgacataga aggtattgga ataggtctct atctttcaag aaaaattata
     2941 tctcttcaag gtggctatat taaagttaca tcagagattg gaaaagggtc aactttttct
     3001 atcttcttac ctaataaata aatttttaaa tatttcattt gtaatgattc tgcaacattt
     3061 tggttttata ctgtatttac aagatgttaa ggaggtatta ttatgagtat tttacaagta
     3121 attaatttaa agaaatatta tggacaaaat tcaaacatta caaaagctct tgatagtgta
     3181 actctatcta ttgaaaaagg cgaatttatt tctattgtag gtacatctgg gagtggcaaa
     3241 tccacgttgt taaatatgat gggaggatta gataatccta catctggaaa ggttattata
     3301 aaaggtaagg aattatcgac tatgactgat gaacaactta caatctttag acgcagaaat
     3361 ataggtttta tatttcaaaa ttataatctt gttcctttac ttaatgtata tgaaaatatt
     3421 atcttaccaa taggattgga tggagagacc atagataagg attttataaa tgaaattata
     3481 aatatgcttt ctcttgaaga aaaattatat gatatgccaa gtaatctttc cggtggtcaa
     3541 cagcaacgtg ttgctatagc tagagcactt gtaacaaaac cagctattat actagctgat
     3601 gaaccaacag gtaaccttga tagtagaact agttcagatg tactgagttt acttaaagtt
     3661 acaagtaaaa aattcaacca aacaattgta atgattacac acaataatga aattgcacaa
     3721 ctaactgata gaattattcg tattgaagat ggaaaaattg ttaactagga ggtgtagtac
     3781 atgtttagaa ataataatcg cacatttata agaaaaatat ctttaaatga tttaaaaatt
     3841 aataagctta aaacatatct ttcaggtatc attataatga tttctacatg tttattatta
     3901 acagtgacct tagtttcata taatgcttcc gtagacttag ttaatgcatc tccatatcat
     3961 gctatatata aatctgtaga tgaaaaaaca aaaaatatac tatatcaaga taaaaacttt
     4021 agtagtgtag gaacttacaa attaattgga agtaataaaa agacagattt tattatgtcg
     4081 attgtttatg cagatgatac agcaattaaa cttatgaatt ttcagccatt gaaaggcaac
     4141 ctgcctacaa ataaaaatga aattgctatt tcagaaaaat atttacagga atttggttta
     4201 gataaagata ttggagatag cattaaatta aattattatg atgatattac aaataaagaa
     4261 gttcaatgtg attttattat tgttggattt ctagaaaatt attatcaaga taatgccaag
     4321 caatactata cagtcgtttc aaatgattat tttaagaata tagcttccgc acctttacaa
     4381 aataaaagta atacttttga cgagtcaata cctgatactg tagatgtatt agttaaacta
     4441 tcagaagaaa atacagagaa agattcagct tcaattaaga cacagttaaa gaaaattgct
     4501 ttatctttag gtattgagaa atataatata tacttaaatg ataactatat tgaatcaaat
     4561 ttaattgatg gtgagcagat tattacagtt ttagtagttg gaatagttgt tctcttttct
     4621 agtgtttttg ttatatatag tattttttat atttcagttg ttaattcagt tcagatgtat
     4681 gctaagttga aatcgttagg tatgacttct tttcagttaa aaaagattat ttctctacaa
     4741 ggaaatattt tgtcaattat ttttattcca ttaggggtta tagcttcttg tataattgct
     4801 tacataattc aaccattagc atggcaaatg atggctgatt tatttataat tttaatttta
     4861 tcactggtga tgtttttaac tgttagaatt tctttattta aaccagttag aattatttct
     4921 aaaatatctg ctattgaagc catgcaatat actgaaacta aatttagaaa aaaagtaaaa
     4981 aattttagtt atataaatat aaaaaatcta gcattaaaga atatcgagtc aaatagaaag
     5041 aaaaatttga ttgcattaat atcactaagc attagtggag ttttatttat ttcaatctct
     5101 aatcttgcta attcaataga tttcaaaaaa caactatctc aacaatttgt ctataacgaa
     5161 gattatataa ttagcattaa gagtgataac ttatatgagc atattacaga aatccaacaa
     5221 aataatccac taacagattc tcttaagaat gaaattaaat ctattcatgg agtaaaaaaa
     5281 ttaattgaat ccaaatcaat taaaagcaat attatagaac cttgcatcaa agatagagat
     5341 gaaaaacaat ttattactct aattaaaggt attacgcctg aactttcaaa atcacttgaa
     5401 gaatatattg taagtggaaa aatagactat acacatttag attcagatag tttaattata
     5461 aataaatata gaacaaaatc ctatggatta aatttaaaag ttggagatac tgttacattt
     5521 aatattttta gtggaaatga tattattaaa aagaaaatga aagttatggg gattattgac
     5581 aattccaata ctggaggtat gttttttgct tcagacaaaa ctttagaatc tctaacgcca
     5641 tataatacaa atttagattt ttctatttta atagataaaa agcattctaa agaagttgaa
     5701 gaaaagttgt taagtataat tcagaaaaat tcaaatttaa tgctttattc atatgaagat
     5761 gattatagaa tgattactag agcatttcaa tatataatta tagcttcata cgtttttgtt
     5821 ggtttaattt cttgctttgg tatactaaat atgaccaata ccctaataaa cagcgtttta
     5881 attagaaaaa aagagtttgc cttacttcaa gccgttggaa tgacaagaaa gcaactgcga
     5941 aatatgcttt atagagaagg tctaaacata agtatcaaag ctatatgtat ttcaagtata
     6001 ctaggttatt ttggttcaaa tctactttgc acttttataa aagatgtaat tagattagac
     6061 tttattaatt ttaaattttc tatatttact attttaattt tttcattcgt attaattggt
     6121 atacaagttt tagtgactga attacttgta agaaatatag aaaaaaatca cttacagaac
     6181 gcttacgttc tcaataagaa tgtatataaa aaatatccat tttttataca agttttaaca
     6241 taatttttta aaagctaaag ggatagaaaa ttttgacttt acataatttt ctgtccctct
     6301 acttgttttt attttataca gatatacttc cttatatctc caaacatctc cagagacata
     6361 aacaatcata ttacttattt tctaaatatg tcaattgcat gacttgtaac ccctactaaa
     6421 tcttcacgtt cacaagtagt aaggtgatat tttaaataca acgcaaatgc atcatcatcc
     6481 atagagtcta ctgcttcacg catatataaa gccaaaccat ctgatgcgac ataatgcagt
     6541 ctagttacat taaatacaga catcaaatca tcaatgtttt ctttacgtac aagctcaaac
     6601 aaatcctttg gctcagactt agcagcaaat gtctgtgggt caatcaaacc tttttcaatg
     6661 tattcagata cattaatatt tcctctatgg aacccctcgt caatgagaca accatcagat
     6721 ataacatatg cagcaaaaac cacaccacca ggttttgtta cacgaatcgc ctcatgtaaa
     6781 gcttgttgct tatcttcttt gttataaaga tgatatagtg gtcctagcaa taacgtaata
     6841 tcatatttat tatctgggaa aatagataaa tccatagcat taccctgagt aatgcttata
     6901 ttttcatttg attgagtatt tctacggaaa acccctatat tatgttcgac caattccact
     6961 gcgtcaacat catatccctg acgtgccagt gtgtgagaat agcgaccagt tgcagcgcca
     7021 atttcaagca cacaatcacc tgattttatg tacttatcaa tgtaatgcat agtagtgaga
     7081 aattccacca ttccatgttt taatgcaagt cgactatctt catcataagt attataaaag
     7141 tcaattaagt attgttttgt ttccataatt tcacctcttc tatattagtt aataaacatt
     7201 aaatagacaa attccaatct ggtttttctt gtcttataaa agctgttaat atatttttca
     7261 acattttttc agcactatat ctatctaatg ttaatttttt tgttgctgtt aagacagcca
     7321 tgccatgagt ataaagaaat aaatctaaaa atattacttt tgcacattcc aactctattc
     7381 caatgctttc cgaaaaaagt cttgcttttt tttcattgtc aatttcttta taaaaatcat
     7441 ttgcctcttc catcatcaaa tctaaatcat ttataaataa caacttaaat aaatgagttt
     7501 cttcttgagc aaattcaatg tatgaaagcg gaagcagtaa ataaggactt acacttctag
     7561 aattacgata attaacaaca tactgattat agtattcata tgcaaaagca agaaattcat
     7621 tttttaattc attcatgttt tcgtaacaag taaaaattgg tcgagtggaa cactgtaatt
     7681 tacttgcaat actcctcgca tttacagctt caaatcccac ttctcttgta atatccaaga
     7741 ctgtattcaa aatcatttct tttgttatct ttactttagg tggcatatat gttcacctct
     7801 ccattaaatt tttaataaaa tacatatgtt atataactat agtaatatat caaagaatta
     7861 tgtcaagaaa aatttttgca tttttttgca attcataaaa ctgataaata aaacatgagt
     7921 tgcaattaat ctattaatag ataacagcaa ctcacatttt cctttaaata ctcttctttt
     7981 tatctaatat ctcatataca caatcttcta ttgaattaat atctttttct tttctttttt
     8041 tagaatcaat taaaacgaaa tacagtataa aacctacagc aagtccccat gttccgcctt
     8101 tagcagcaat tacacctccc ataagtccaa tcaatccctt ctcaatacct gtatcacata
     8161 gctccattga tagctttgta cataaatatc cttgaactaa aagtgttact gacaatgcta
     8221 atggtataag aggtgtagca atctgcgcaa ttggataaaa acagataaaa attgctgaaa
     8281 cccatactaa tgtacccata ccactaaaaa cagactgcat ggcttccttt ccaaccttgt
     8341 acctttgagc aacacttcct gtaagtgtcg cagctaatgg accgcacatt ggtatatatg
     8401 gagaaatgat tgccataaaa atatttctta tcccacttat aacattagac ctatttgaat
     8461 tgaaatctat atattcatct tttctatttt cactttcaga aagtactaaa ttttcacctg
     8521 ttataaaatc accaaatgca attatgtata tcataaaagc cattggtatc ccttgtataa
     8581 ataacattac acttggaaaa cctattccaa aaggagatac tgtatttatc aaatttttaa
     8641 aatctactat gtaaactaaa ttatcaaaac taaatctagg aatttctatt tctttactaa
     8701 atatacctac tattaaactc actaaaatag ctgatactac accatattcc cctaaatgaa
     8761 ataaaaattt attcttaact tttagagtat caaatctttc agaaaacatt acaaaacaag
     8821 caaccaaaat tcctactgtt atagatatag gataagaatc aaatctccct ttaggattaa
     8881 actctgatat tatagtcgat ataccgacac ccattagtat tccaccttta atagaatttg
     8941 gtatcttact aactaacttt ccagctacac cagttatacc cattaaaata aatattattc
     9001 ctaaatctat ttgtaaagct gttaatgctt gtattctttc aatgcccatg ctgaacttta
     9061 acaaatatgc agttgtaaga gttatagaag ctgtaatcca gccaggtaca actgggtctc
     9121 ccataaaaac atgcaagcag tataatattg agttaatgat agccatactc catgccactt
     9181 catagggtaa tcccaaaatt tgtgtactaa gaccaattgt gcctaaactt gtcatacttg
     9241 atattatgcc ttgaatagct tctggtttct caaatctgta atgtataaat ggaattctta
     9301 tcttgaacat tccaaatttt atatgaggtt gttcccttct agcgagtctt tccataactt
     9361 cactccacct ttcaataact cttataagtt tacaacttta taattgttta taaattatca
     9421 atgtcgttaa aattagtata taacatgcaa attatttaaa caataaatta aatttataaa
     9481 attgatagat tttgtgcata tatagaaaga cctcttcatt tttagttatt taaatcactt
     9541 gataaaggtt ttttataaaa ttcaatagtt ctattccatg attcaaactg tccttttgaa
     9601 tttccttcta cagttccacc tgaagaaaaa acccccccac tcatactcat acataaagtc
     9661 tcctttagag gcataatata tggaattggt aacggatgtc ccatattatt gtatgttaat
     9721 aattctacgt tatatggata attattttct tcaaggattt ctaatgtctc taaacaagct
     9781 tcataactat tccatatgtt atcttctttc ccagcaatca taagtatatc tgctttggaa
     9841 ttttctattt ttattctagc ctcttttcta ttctctgctc ttttaagact tttcttataa
     9901 gtactattaa atccaaatgg aatacctttt gatatatttt ttttctgttc ttcaaagaat
     9961 atatcattgt ctaccttgat atatggaaat ggctctcctt tatatgacca tgatgaagta
    10021 tcatttccac tcatcaatcc attaagagct tggaaacaat aagtgtgagg ttgtgatact
    10081 acaatctttt taatttgttc atatattgaa gcaagtaaaa aggccaattc tccacccttt
    10141 gatgttccat gtacaaatat ttctttagta ctagtaattt cattttcact aatccactca
    10201 aatacttttt caaaatattc taatggaacc tcttctaatt tatctggtaa cccttctaaa
    10261 gcaaagtatg caactgttag tacattgaat cctctagatg caagtggagc cgctaaaagg
    10321 tctagagcgt ctaaatttcc atcagaacca cctagcatta aaattgtttt attattagaa
    10381 ttttgtttat aaaataattt tcctataaac tcatcagata tgtattttgt ggtaatatct
    10441 tctgatttaa atattctctt taattcaact tcttttcttt cagtagaagt ttcaaatatc
    10501 atattcataa ttattggttt atcaatagat atattttctg caaaattttt accttcagtt
    10561 ttagattttt ccaacgaata aattaacccc atacaattat cagccttata agtaccttct
    10621 ataggttcaa ctaaatctaa atctatttct cctttttcat ttgaacaaaa cactccataa
    10681 gatgaaaatt cttctccctt gcaccatgga aattccattt tcacacttat tttaagttta
    10741 gtatttggaa ttaagtctga aaccataatc ttaagttttt catccacaaa tgattctaca
    10801 ttgtatacat ttactttcat aatctatttc tccttataat aattatttat gctttatata
    10861 taaaacagct ttatctaata cagcataaat aaatttttaa attaagacac tatgttgact
    10921 attctatttt atcaacatat ctcatctttg tcaacatatt gtcttttctg gcaatttaaa
    10981 attatgtagt ctatatgaaa tccagttgat ttttggctaa ttaaagatag gaatgctatt
    11041 aggtttagaa tatatctatt caaaataaaa aaccactcat tttaagaatg gtttttaaca
    11101 taaactattt gttattaata aggatatttt ttatttaaat ttatataatc gaacatctaa
    11161 tatttaatgt ttaataaaat ttatgcattt gcaaaagtat atatggcatt aactcttgct
    11221 acatataaat tctcatcact acatccaata tatctagcat atttttcaat cttttctttt
    11281 gcattttcaa catcattttc atcgatttcc attaactcat ttatataaat atccattagt
    11341 gatgctttat attctcttat atcatagcct aaaaattcag tcaatctctc aatttcctta
    11401 ctaaaatctc catcttcttt taccaaaaat tcgtatacat cttctctata tggatttaaa
    11461 gttattattt gttttactac tttgcattcg tcagccttcc caataaaacc atctttataa
    11521 ttattaaata aggcattaga tttttttata gattcttcat caatataaga atgtaccgca
    11581 gttatacgat tttctataag ggcatctaca acagcataat gaattttaaa acaatcattg
    11641 tataaataat tgctcatttt ctttattgtt gattctattt ctaagaagct agcatgacca
    11701 ttttttttat ttttatttgg tataataaaa gttttattca aattattaaa aagtctctta
    11761 aaatctaaat atttttcaca ataaacactt ttaaaggtat cgatatctat agttataatt
    11821 ccaaaactta ctatagtctc aattcctttc ttaaaagact cttctatata tttttctcct
    11881 aaacttaaac ctctatcaga tatttgcttt agattttgaa aactatttaa acaatctctc
    11941 tcaaagtttt tagatatttc taaagcatat aatttaaaga ttttccttat cttattataa
    12001 ttattttttg tatcatcaaa tctgataata gctccattta catcaaaagt aatcattgag
    12061 ttttaacctc cccattagtt tca
//
LOCUS       DAEPXF010000113        12076 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain HB27
            SAMN13376389-rid12479423.denovo.119, whole genome shotgun sequence.
ACCESSION   DAEPXF010000113 DAEPXF010000000
VERSION     DAEPXF010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13376389
            Sequence Read Archive: SRR10518432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 12076)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12076)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001069624.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001069624.1
            Genome Coverage       :: 315x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/15/2021 01:54:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,165
            CDSs (total)                      :: 4,076
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 89
            rRNAs                             :: 10, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 10 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 19 of 69
            Pseudo Genes (incomplete)         :: 44 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 10 of 69
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12076
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13376389-rid12479423.denovo.119"
                     /strain="HB27"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="China: Hebei"
                     /collection_date="2017"
     gene            60..719
                     /locus_tag="KRL67_002630"
     CDS             60..719
                     /locus_tag="KRL67_002630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HBH1523496.1"
                     /translation="MTVVLIRSIILYITVLIALRVMGKGEIAEMNCFDLVITLLIAEV
                     ASVPMENNNIPIINGVAAITGLVIMQTLISFLSLKSRRLSSFLSGKPSVLIDKGKIVY
                     KELKKERVTIDELLEQLRVQGYFNLKDVQYAILETDGNLSVVPASTYNSTPPKAFNHL
                     PIPLILDGRIINKNLDIAEKDTNWLMGILKSNHIETFKDVLICVLDENDKIFIQNKKG
                     D"
     gene            722..1102
                     /locus_tag="KRL67_002631"
     CDS             722..1102
                     /locus_tag="KRL67_002631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4363 family protein"
                     /protein_id="HBH1523497.1"
                     /translation="MKSATFVILWTILFVLFGFYINNKLYDFTDEYKDNISVLEKSIE
                     NEDWEKAQKEADSISTRWAKEKNHWYKVLNHEYFDEIGLKFNILDKAIYTENKLKSLE
                     EVEGIKTYLGNIVESVKFDVNYIF"
     gene            complement(1191..2354)
                     /locus_tag="KRL67_002632"
     CDS             complement(1191..2354)
                     /locus_tag="KRL67_002632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HBH1523498.1"
                     /translation="MRTVKQVSDLTGISVRALHYYDEIGLLKPNKITDAGYRLYDDES
                     IKTLQQILFFKEIDIPLREVKEIMSSQYFDKVEALKNQRQLLILKRKRLDELIELINQ
                     TLRGEGNINFKEFDMSEYFDVLEEFKREHRNKIIKIYGSVEKYNEYIERVKSNEEKIA
                     KMAIKQYGTIEKFAQAIKTNFSSDILNLGEKFDRYKNDCLKEQHPKLKELYRKLVEDL
                     NKNPSSTELQEIAKEITDISKKDYEIFSMDTGDDNWYYMVQNYLVNPMWIEEVDKKYG
                     SGAGKFIGQVLKTYLRDRKPKINTLYEKLVEDLSRDCSSREIQSIVEEIDNEMKRSNE
                     FYKIDNGERYFDYMSELYLQDSNYIKVTDKNYGDGASKFIGEAFKIYFDNNNC"
     gene            complement(2447..3508)
                     /locus_tag="KRL67_002633"
     CDS             complement(2447..3508)
                     /locus_tag="KRL67_002633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4872 domain-containing protein"
                     /protein_id="HBH1523499.1"
                     /translation="MRKIIEIKHHSHDYECMWNGIEDLYINKTGECLPNDFFFLLSGF
                     GSFCYIKTNKADLKRMVALGDGRTKQMYEFLAPIVGFEYKHHEFKKYEQALKKAKSEI
                     DGGYPVILGALDMYYLPYYKKLYHKEHIPFHYILMVGYDDEGIYLYDCGRTELLHLSY
                     DKLRQSMNCNYPGLSKENTICTIRMNTLKNKYNIAREAIAIKRDMFLSPPKGFLGYKG
                     FEKFIKELPQWNTELDKDDYDKILTNMVMFFGTVPTIPNAIKGIKEPDTVQFKGGFDK
                     MSRMLNSIGEEYKNNSFIQVAEIFDKTAIIIEKISNIIIDYLTQTCDDTEQLPLLFSE
                     VLEHMKTGYLILDVQKIYC"
     gene            complement(3757..4956)
                     /locus_tag="KRL67_002634"
     CDS             complement(3757..4956)
                     /locus_tag="KRL67_002634"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HBH1523500.1"
                     /translation="MKEKVVLAYSGGLDTSIIIPWLKENYEDIDVIAVCGNVGQEDKM
                     EDVYEKALQSGALKAYVDDISEEFVTETIFKAVKAEAKYEGKYLLGTSLARPIIAKKL
                     VEVAHKEGAKYICHGCTGKGNDQVRFEATIAALDPTIKVIAPWRIWDIKSREDAIDYA
                     EKHNIKVTATKAKIYSVDANLWHVSTEGGDIEHLENEHKKDVYKQCVDPEDACDVAEY
                     VEVYFEKGVPKKVNGEELSPVALIHKLNELGCKHGIGVIDIVENRLVGMKSRGIYETP
                     GGTILYEAHNILESATLDKDTLHFKQMVSYKYGELIYNGLWYCKLRESIDAFMEQTQD
                     NVTGTVKVKLYKGNIKPAGIFTENALYDEGISSFGNSELYDHKDAEGFINLFTLPLKI
                     RAMKAGK"
     misc_binding    complement(5209..5416)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(5527..6264)
                     /gene="truA"
                     /locus_tag="KRL67_002635"
     CDS             complement(5527..6264)
                     /gene="truA"
                     /locus_tag="KRL67_002635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011010156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HBH1523501.1"
                     /translation="MRNIKMIVAYDGSRYKGYQKLGDNNMTIQEKLENVLSKMTNETV
                     EIIGSGRTDMGAHARGQVVNFRTNCMDSLDKIQKYLYEYLPEDIVVKTVEEVDERFHS
                     RYNVKSKTYMYKIDNNKYHNPFIRKYATHVSKKLDLDRMRKASEYLVGEHDFTSFASS
                     KSKKKSNVREIYSINIKEDDNVIEIYVEGNGFLYNMVRIIVGALIDVGLKRRAPQDIK
                     YMLESKDRCQSSDTAPAKGLCLWKVTY"
     gene            complement(6286..7719)
                     /locus_tag="KRL67_002636"
     CDS             complement(6286..7719)
                     /locus_tag="KRL67_002636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBH1523502.1"
                     /translation="MLHFEGLRKKVIKNYFIIIIIMVTLFEGLFMFYIQNYYYDSVKQ
                     LLESEIKYADEYNAITMETTSFEKKVKNIFDKQPLTKNSEFGISIIDKDKNIILDQYG
                     FKSKEKANYEDVNNALKDIKTKNLTPYTYRIPDTGEHVMSISLPLKVNNIIEGVVRYT
                     VSLDAIDNAILKQATWLILAGIFILIIAILISLKFAETLIKPLRELKKFANELAVGNY
                     NIKLEKMKIVDDEIGDLAQTFEHMAHEIDKSEKLKEEFISSVSHELRTPLTSIKGWSE
                     TLGYESITREELDLGLGIIQDETERLIKLVEELLDFSRLSSDRIKLHVDIVDVEGLIV
                     GVVNQLKVKAAEKDISLLFEFENEFIENIQGDKNRLRQVLINLIQNSFKFTSQGGYIK
                     VVASQDEEITTISVEDNGSGIEKQNLNKVLDKFFQEDYNKAGSGLGLAISNEIVKLHG
                     GRMKIESEKNVGTKITFNIKNKFAKQA"
     gene            complement(7722..8420)
                     /locus_tag="KRL67_002637"
     CDS             complement(7722..8420)
                     /locus_tag="KRL67_002637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBH1523503.1"
                     /translation="MKEKILILEDEIGIRSFVSINLKREGYEIVEAGTGREAIEKMTT
                     EKDITIALLDVMLPDISGIEVCKFIRENFDQVGIIMLTAKAQEDDKIEGFISGADDYI
                     IKPFSIKELLVRVSALLRRVAKDDSSVKSSEIVSPPFILDIDKRKLFKNGKEIELTPT
                     EFSIVKYLISNAKQSLSRDQILDEVWGTNYLYDFKIVDVNIRRIRNKIEDDPSKPKYI
                     QTIWGYGYCFRKEE"
     gene            complement(8449..9837)
                     /locus_tag="KRL67_002638"
     CDS             complement(8449..9837)
                     /locus_tag="KRL67_002638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH1523504.1"
                     /translation="MKVLKFITVVALMMLFVTGCNIKTESPEQLAKIPDYDDTKKVLY
                     DGIDQLLKPDSSMILPSNTKEVGKINKVDLNSDGIDELVVFEQKEDLSNNVSQVGFVT
                     LSYNGEKYVLGDHFLENGESIEYANFYDLDSDGYKEVILLVKSKDKTNMYIYKVVDNE
                     ITKIYDLDASWLQNKEDFNDMKVKIGYIDGDDKLDILMLHLNNKTNEMFASVANFDGK
                     MEIKDSVKFENVKNLSELYITLGNVASSVGNSSAAIKGIVLDIPILKDNNYITQILYM
                     KDGKINKAFSDYDKTITKSYYIPVDDIDKDKIIEIPIVAGSTGNNRNTYSSKSSANIS
                     WYRWNGKEGADSSLIFTSQIYYNYKYNFKLFIPNNLFDKILVEQEFVGEKAVFKFYYF
                     DYSDRKNLFNIVVESKNKLEDRKNTGTQNSIVLQESDEYTFLLVVNNANEMDKLDMTI
                     DALKEYFSLIYG"
     gene            complement(9973..11121)
                     /locus_tag="KRL67_002639"
     CDS             complement(9973..11121)
                     /locus_tag="KRL67_002639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HBH1523505.1"
                     /translation="MKNYTLISPCFFGMEKMLAREITNLGYEIIKTEDGRITYKTDEF
                     GIAKSNMWLRCAERVHLKIAEFEAKSFDELFENTKRINWSRYIPYGAQFPISKASSIK
                     SKLYSTPDVQAIVKKAIVESLKKSYLEDGLLKEDKEKYPIFVFIHKDKVTISIDTTGD
                     ALHKRGYREKANKAPIRETLAAGLIYLTPWKAGRVLVDPMCGSGTILIEAAMIGINMA
                     PGLNREFISEKWRTLDKKIWWDVRKDAFNKIDNESKFKIYGYDIDEESIDIARENAEI
                     AGVDEYIEFNVGDATQFKSEDEFGFIITNPPYGERLEDKDSVKQLYKELGYAFRKLKN
                     WSYYLITSYEDFEYEFGQKADKKRKLYNGMLKTNFFQYPGPKPPRNNK"
     gene            complement(11176..11994)
                     /gene="larE"
                     /locus_tag="KRL67_002640"
     CDS             complement(11176..11994)
                     /gene="larE"
                     /locus_tag="KRL67_002640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent sacrificial sulfur transferase
                     LarE"
                     /protein_id="HBH1523506.1"
                     /translation="MEVSFVKEKEKLDKLKKRLLELGSVVVAYSGGVDSNFLLKVAKD
                     TLGENVIAVTIHAMMHSSREIEEAKQYTQNFGVKHIILNIENFDLKEFKENGVDRCYH
                     CKKYIFSKIKEVAKEHNIKYIVDGTNMDDLGDYRPGLKALSELGVISPLQDSSLKKEE
                     IRSLSKTLGLNTFNKPSFACLASRIPYGVEITDENLRIVEKSEEYLFNLGFSQFRVRM
                     HGDIARIEVGQEELGKFFENNNFNKVDTKLKIFGFKYVTLDMSGYKMGSMNLNV"
BASE COUNT         3889 a         2109 c         1216 g         4862 t
ORIGIN      
        1 tttttatata ggacatacta actaagtgta aatatttttt aaaggagtaa ttgatagata
       61 tgacagtagt attaataaga agtattatat tatacataac agtactaatt gcacttagag
      121 tcatgggaaa aggtgaaata gctgaaatga actgttttga tttagttata acactattaa
      181 ttgcagaagt tgcatctgtc ccaatggaaa ataataatat accaattata aatggtgttg
      241 cagcaataac tggacttgta attatgcaaa ctctaatttc atttttatca ttaaaaagca
      301 gaagattaag ttcattttta tcaggtaaac cttctgtact aatagataag ggaaaaattg
      361 tatataaaga attaaaaaaa gagagagtca ctatagatga gttattggaa caacttagag
      421 ttcaaggtta cttcaattta aaagatgtac aatatgctat tttggaaact gatggtaacc
      481 taagtgttgt tccagcatca acttataata gtactcctcc aaaagctttt aatcatttac
      541 caattccact tatattggat ggtagaataa taaataaaaa tctagacata gctgaaaagg
      601 atactaattg gttgatgggt attttaaagt ctaatcacat agaaactttt aaagatgttc
      661 ttatttgtgt tttagatgaa aatgacaaaa tatttattca aaataaaaag ggtgattaat
      721 attgaaatct gcaacatttg ttatattatg gactatctta tttgtattat ttggatttta
      781 cattaataat aagctatatg attttacaga tgaatataag gacaatatat ctgttttgga
      841 aaaatctata gaaaatgaag actgggaaaa agctcaaaaa gaggccgatt ccatatctac
      901 aagatgggct aaagaaaaaa atcattggta taaagtttta aatcatgaat actttgatga
      961 aataggatta aagttcaata ttctcgataa agcaatttat actgaaaaca aattaaaatc
     1021 tttagaagaa gttgaaggta taaaaacata tttgggaaat atcgttgaaa gtgttaagtt
     1081 tgatgtaaac tatatatttt aataacttta aataaggata tagacagaac taatctatat
     1141 ccttattttt gtgcttttat aaagcttaca cattttttac ttccaaattt tcagcaatta
     1201 ttattatcaa aataaatctt aaaagcttct ccaataaact tagatgcacc atcaccataa
     1261 ttcttatcag ttacttttat atagttagaa tcttgtaaat aaagctctga catataatca
     1321 aaatatcttt cgccattgtc tattttataa aattcattac ttcttttcat ttcattgtct
     1381 atttcttcaa caatactttg aatttccctt gaagaacaat ctctactcaa atcttctaca
     1441 agtttttcat acaatgtatt tatctttggt tttctatctc ttagataagt ctttagtact
     1501 tgtccaataa acttacctgc tccacttcca tactttttat ctacttcttc tatccacata
     1561 ggatttacta gataattttg aaccatataa taccagttgt catcaccagt atccatagag
     1621 aaaatttcat aatctttttt agatatatct gttatctctt ttgctatttc ttgaagttct
     1681 gttgaagaag gattcttatt taaatcttct acaagttttc tatataattc ttttaatttt
     1741 ggatgttgtt cttttaaaca atcattttta tatctatcaa atttttctcc taaattcaat
     1801 atatcactgc taaaatttgt ttttatagct tgagcaaact tctcaatagt tccatactgt
     1861 tttatagcca ttttagcaat tttttcttca ttggatttaa ctctttctat atattcattg
     1921 tatttttcta cactaccata aattttaatt atcttattcc tatgctctct tttaaattct
     1981 tctaaaacat cgaaatactc actcatatca aactctttaa aattaatatt accttctccc
     2041 cttaaagttt gatttataag ttctataagc tcatccaacc tttttcgttt taaaataagt
     2101 aattgtcttt gattttttaa agcttcaacc ttatcaaaat attggcttga cattatttcc
     2161 ttaacctctc ttaaaggaat atcaatctcc ttaaaaaata aaatttgttg aagagtttta
     2221 atagattcat catcataaag tctatatcct gcatctgtaa ttttatttgg ttttaaaaga
     2281 cctatttcat cataataatg taatgccctc acacttattc cagttaaatc agaaacttgt
     2341 ttcactgttc tcatatccac acctcaaatt ttaattttac agctatcaat acctactaat
     2401 atcttcaagc tatattttca tattacacta ttacgtaatg ttagggtcaa caatatattt
     2461 tttgtacatc caagattaaa tatcctgttt tcatatgctc taagacctca gagaataata
     2521 acggaagttg ctctgtatca tcacatgttt gagttaaata atctattata atgttggata
     2581 ttttctcaat tataatagca gttttatcaa aaatctcagc tacttgtata aaagagttgt
     2641 ttttgtattc ctccccaata ctgttgagca ttctactcat tttatcaaat ccacctttaa
     2701 attgaacagt atctggttct ttaattcctt tgattgcatt tggaattgtt ggtactgttc
     2761 caaaaaacat aaccatattg gtcaaaatct tatcataatc atctttatcc aattcagtgt
     2821 tccactgtgg caactcttta ataaactttt caaaaccttt atatcccaaa aatcctttag
     2881 gtggacttaa aaacatatca cgctttatag caatcgcttc tctagcaatg ttatatttat
     2941 tctttaaagt attcattctt attgtgcaaa tagtattttc tttgcttaat ccagggtaat
     3001 tgcaattcat gctttgacgt aatttatcat aagaaagatg taataattct gttctaccac
     3061 aatcatacag atatattcct tcatcatcat atcctaccat taatatataa tgaaaaggaa
     3121 tgtgttcttt atgatatagc tttttataat atggtagata atacatatct aaagctccta
     3181 aaataactgg atatccacca tcaatttcag attttgcttt ctttaatgct tgctcatatt
     3241 ttttaaactc atgatgtttg tattcaaagc caacaattgg tgctaaaaat tcatacatct
     3301 gttttgtcct tccatctccc aaagctacca ttcttttcaa atcagcttta tttgttttaa
     3361 tataacaaaa cgaaccaaaa cctgatagta aaaagaaaaa atcatttgga aggcattcac
     3421 ctgttttgtt tatatataaa tcttctatac cattccacat acactcataa tcatgtgaat
     3481 gatgtttaat ttcaattatt tttctcatat aatctatctc ctcactataa attttcatta
     3541 gattcagtat aaaccctctt acaatgagag agtcaataac tattgtattt ataaaattta
     3601 tattttaggc tttatatttt cacattatta agctatttgg gactataata tattttgtta
     3661 tactatttac tgtataattt aataaattca aataaaaaaa aagagcatat aaaaacttaa
     3721 aaatcgtttt tatacactct taaataattt attttattat tttccagctt tcatagctct
     3781 gatttttaat ggtaaagtga ataaattaat aaatccttct gcatctttgt ggtcataaag
     3841 ttcactattt ccaaaagaag aaattccttc atcataaaga gcattttcag taaatattcc
     3901 agcaggtttt atgtttcctt tgtaaagttt aacttttact gttcctgtaa cattatcttg
     3961 agtttgttcc ataaatgcat ctatagattc tcttaattta caataccata atccattgta
     4021 aataagttct ccgtatttat aagataccat ttgtttgaaa tgtaatgtat ctttgtctaa
     4081 agtagcactt tctaaaatat tatgtgcttc ataaaggatt gttcctcctg gagtctcata
     4141 tatacctctt gatttcatac caacaagtct attctcaact atatctataa caccaattcc
     4201 atgtttacaa cccaactcat ttaacttatg aatcagtgca actggagata attcttcgcc
     4261 atttaccttt ttaggtacac ctttttcaaa gtaaacttct acatattcag ctacatcaca
     4321 tgcatcttct gggtctacac attgtttgta aacatctttt ttatgttcat tttctaggtg
     4381 ctctatatcc ccaccctctg ttgacacatg ccaaaggtta gcatctactg aatatatttt
     4441 agctttagtt gctgttacct ttatattgtg tttttcagca tagtctatag catcttctct
     4501 tgattttata tcccaaattc tccaaggtgc aataactttt atagttgggt caagagctgc
     4561 aattgtagct tcaaatctaa cttgatcatt accttttcct gtacatccat gacatatata
     4621 ttttgcacct tctttatgag caacttcaac taatttttta gctattatag gtcttgctaa
     4681 agaagttcct aataagtatt ttccttcata tttagcctct gcttttacgg ctttaaatat
     4741 agtttcagtt acaaattctt cacttatatc atccacatat gcttttaatg ctccactttg
     4801 taaagctttt tcatatacat cttccatttt atcttcctga cctacatttc cacaaacagc
     4861 tataacatct atatcttcat aattttcctt taaccaaggt attatgattg atgtgtcaag
     4921 tcctcctgaa tatgctaata ctactttttc cttcattttt aattcctccc aatcttaaat
     4981 tataattttt attttataga aattcttaat attgaatgac tctgagaatt tagactcaca
     5041 taaaatcatt ttaatactaa aaaaacccct tagtatttta aaatactaag aggcgaaata
     5101 tccgcggtac cactcttatt gataaactta gctaatcaat tagtaaatgt tcaacatact
     5161 agataaacac taataattaa atatattaaa aaacgcctct tggaaaaacc aagagacgaa
     5221 atatatccgc gttaccactc taattgataa gataaactta tccactcttt ctcttaaggc
     5281 gagattacct actaacatac taaattcaat tagtatcctc caaagttctt ttcactaata
     5341 cttatttgcc aagcttccac catcctcagc tctctataaa acacataaaa gttactctct
     5401 ttctcacagg atttaaatat aaagttttat atctctctta taaaatatat aatagtttat
     5461 ttttccattt atgtcaacta tataatttat tttattttta ttttaaagag agttttatat
     5521 aattgactaa tatgttactt tccaaagaca taatccttta gctggtgcag tatctgatga
     5581 ttgacatcta tcttttgatt caagcatata cttaatatct tgtggagctc ttctttttag
     5641 accaacatct atcaaagcac ctactattat ccttaccatg ttatacaaaa atccatttcc
     5701 ttctacatat atttctataa cattatcatc ttcttttata tttatagaat aaatttctct
     5761 tacatttgat tttttcttag atttagaaga agcaaaacta gtaaaatcat gttctcctac
     5821 taaatactca cttgcttttc tcattctatc taaatctaat tttttactta catgagtagc
     5881 atattttctt ataaaaggat tatgatactt attattgtca attttataca tataagtctt
     5941 tgattttaca ttgtatcttg aatgaaatct ttcatcaact tcttcaacag tttttacaac
     6001 tatatcttct ggtaaatatt catataaata tttttgaatt ttatccaaag aatccatgca
     6061 attagttcta aaatttacca cttgtcctct tgcatgagct cccatatcag ttcttccaga
     6121 acctattatc tccactgttt cattagtcat tttacttaaa acattttcaa gtttttcttg
     6181 aatagtcata ttattgtcac ctaatttttg atatccttta tatcttgaac catcgtatgc
     6241 tactatcatt tttatgtttc tcatcttaat cctctttcta agaatttatg cttgtttagc
     6301 aaatttattt tttatattaa aagttatttt tgttccaaca ttcttttcac tttctatttt
     6361 cattcttcca ccatgaagtt ttacaatttc attactaatt gctaatccta atccactacc
     6421 agccttatta taatcttctt ggaaaaattt atctaacact ttatttaaat tttgcttttc
     6481 tatacctgaa ccattatcct ctactgatat ggttgttatc tcttcatctt gagaagctac
     6541 aacttttata taaccacctt gactcgtaaa tttaaatgaa ttttgtatta aatttatgag
     6601 aacctgtctt aatctatttt tatctccttg aatgttctct ataaattcat tttcaaattc
     6661 aaataaaagt gatatatctt tttcagctgc tttaacttta agttgattta caacacctac
     6721 aattaaacct tcaacatcaa ctatatcaac atgaagtttt atcctgtctg aagaaagcct
     6781 tgaaaagtct aataattctt caactagttt tataagtctt tctgtttcat cttgtataat
     6841 gcctaaacct aagtctagct cttctcttgt tatactttca taacctaagg tttcactcca
     6901 cccctttata gatgtaagag gagtccttag ttcatgagat acagatgata taaattcctc
     6961 ttttaatttt tcacttttgt caatctcatg tgccatatgt tcaaatgtct gtgctaaatc
     7021 accaatttca tcatctacaa tcttcatctt ttctaatttt atattataat ttcctacagc
     7081 caattcattt gcaaatttct ttaactctcg taatggtttt ataagagtct cagcaaattt
     7141 aagacttatt agtatagcta taataagtat aaatattcct gctaatataa gccatgtagc
     7201 ttgctttaat attgcattat caatagcatc taatgatact gtatatctaa caacaccttc
     7261 tattatatta tttaccttaa gtggtaacga gatactcata acatgctccc ctgtatctgg
     7321 tattctatag gtatagggtg ttaaattttt agtttttata tcttttaatg cattgttaac
     7381 atcttcatag tttgcttttt ctttactttt aaaaccatat tggtctaata taatattttt
     7441 atctttatct attattgaaa ttccaaattc tgaattttta gttaagggtt gtttatcaaa
     7501 tatatttttt acttttttct caaaacttgt agtttccatt gtaatagcat tatattcatc
     7561 agcatatttt atttcagatt caagtagttg tttgacagaa tcataatagt agttttgtat
     7621 gtaaaacatg aacaatcctt caaacagagt aaccattata ataattatta taaaatagtt
     7681 ttttattact ttttttctca gaccttcaaa atgtaacact attattcttc ctttctaaaa
     7741 cagtatccat aaccccaaat agtttgtata tattttggtt ttgatgggtc atcttctatt
     7801 ttatttctta tccttcttat atttacatct actattttaa aatcatataa atagttagtt
     7861 ccccatactt cgtctaatat ctggtctcta cttaacgact gtttagcatt tgatattaga
     7921 tatttcacta ttgaaaattc agttggtgta agctcaattt cctttccatt tttaaataat
     7981 tttcttttgt ctatatcaag tataaatggt ggagaaacta tctcactcga cttaacactt
     8041 gaatcatcct ttgctactct tctaagaaga gcagaaactc tgactaaaag ctcttttata
     8101 ctaaatggtt ttatgatgta atcatctgct cctgaaataa atccttctat tttatcatct
     8161 tcttgagctt tagcagtaag cattattata cctacttggt caaaattctc ccttataaat
     8221 ttacatactt ctataccact tatatctgga agcatcacat ctaaaagagc tattgtaata
     8281 tccttttctg ttgtcatttt ttctatagct tctcttccag tacctgcctc aactatttca
     8341 tagccttctc tttttaagtt tatactaaca aaacttctaa taccaatttc atcttctagt
     8401 ataagtatct tttccttcat aaaataaaac ctccaattga gtataatatt aaccatatat
     8461 taatgagaaa tattctttta atgcgtctat agtcatatct agcttatcca tttcatttgc
     8521 attgtttaca actagtaaga aagtatattc atcgctctct tgaagtacaa tagaattttg
     8581 agtacctgta ttttttctat cttcaagttt atttttgctc tctactacta tattaaataa
     8641 attcttacgg tctgaatagt caaaatagta aaatttaaat accgcttttt ctccaacaaa
     8701 ttcctgttca acaagtattt tatcaaataa attatttgga ataaataatt taaaattata
     8761 tttataatta taatatatct gacttgtaaa tattaaactt gagtcagcac cttcttttcc
     8821 attccatcta taccaactta tatttgcaga tgatttagaa ctataagtat ttctattatt
     8881 acctgtactg cctgcaacta taggtatttc tataatctta tctttatcaa tatcatctac
     8941 aggtatataa taagatttag taatagtttt atcataatca ctaaaagctt tgttaatttt
     9001 cccatctttc atataaagta tctgcgtaat atagttatta tctttcaata taggaatatc
     9061 aagtacaata ccttttatgg ctgcagatga atttccaaca gatgaagcaa cattgcctaa
     9121 tgttatatat aactcactta ggttttttac attttcaaac ttaacagaat ctttgatttc
     9181 cattttccca tcaaaatttg caacacttgc aaacatttca ttagtcttat tattcaaatg
     9241 aagcatcaat atgtctaact tgtcatctcc atctatatat ccaattttta ctttcatgtc
     9301 attaaaatct tctttatttt gaagccatga tgcatccaag tcatatattt ttgttatttc
     9361 attatctact actttatata tatacatatt cgttttgtct ttggatttaa ccaataagat
     9421 aacttcctta tatccatctg agtccaaatc ataaaaatta gcatattcta ttgattctcc
     9481 attttctaaa aaatggtctc caagtacgta tttttctcca ttgtaactca aggttacaaa
     9541 ccctacctga ctcacattat tacttaaatc ttctttttgt tcaaatacta ctaattcatc
     9601 aattccatcg ctgtttaaat ctaccttatt tatttttcct acttcttttg tattagaagg
     9661 caatatcata gatgaatctg gttttagtaa ctggtcaata ccatcataca atactttttt
     9721 tgtatcatca tagtcaggta tcttcgctaa ttgttctgga gattcagttt ttatattaca
     9781 tccagtaaca aataacatca tcagggctac tacagtaata aactttaaca ctttcattta
     9841 actcctccta atcctataaa tctttaaatt tgttcacaaa aatctcctca caataattag
     9901 acgtttcaag gcgtcataat gttttactta ttttaataaa attttatatt ttacaaatga
     9961 atcatgtaaa acttatttat tatttctagg aggcttagga cccggatatt ggaaaaagtt
    10021 agtttttaac ataccattat acaattttct ttttttgtct gctttttgcc caaactcata
    10081 ttcgaaatct tcataagatg ttatcaaata atatgaccaa ttttttaatt ttctaaatgc
    10141 ataaccaagc tccttataca actgtttaac actgtcttta tcttctaatc tttccccata
    10201 aggaggatta gtgattataa acccaaactc atcttcactt ttaaattgag ttgcatctcc
    10261 tacattaaac tctatatatt catctacacc tgctatttca gcattctctc tagctatatc
    10321 tatagattct tcatctatat catatccata aatcttaaat ttagattcat tgtctatctt
    10381 attaaaagca tcttttctta catcccacca aattttttta tcaagagttc tccacttttc
    10441 tgatataaac tctctattta agccaggtgc catatttata ccaatcatag cagcttcaat
    10501 caatatagta ccagaaccac acatagggtc cactaaaact ctacctgctt tccaaggagt
    10561 taaataaata agcccagcag ctaaagtctc tcttattgga gctttatttg ccttttctct
    10621 atatcctctt ttatgcaaag catctcctgt tgtatctata gaaatagtta ctttatcttt
    10681 gtgtataaat acaaaaatag ggtatttctc cttatcttct ttaagcaaac catcctccaa
    10741 ataacttttc tttaagcttt ctactatagc cttttttact atggcttgta catctggtgt
    10801 actgtataat tttgatttta tagaggaagc ttttgatata ggaaattgag ctccataagg
    10861 tatatatcta gaccagttta ttctttttgt attttcaaat aattcatcaa agctttttgc
    10921 ctcaaattca gcaattttaa gatgtactct ttcagcacat ctcaaccaca tgtttgactt
    10981 tgctattcca aattcatcag ttttatatgt tatccttcca tcttctgttt ttattatttc
    11041 atatcctaaa ttagtaatct ctctagccag cattttttcc attccaaaaa aacatggcga
    11101 tattaatgta taatttttca taaaacctcc tgtttcctta tattaactat aaaacttatt
    11161 tatttttaaa ataatctaca catttaaatt catacttccc attttatatc ctgacatatc
    11221 tagtgtaaca tatttaaagc caaatatttt taatttagta tcaactttat taaaattgtt
    11281 attttcaaaa aatttaccta gttcttcctg tccaacttct attctagcta tatctccatg
    11341 cattctcact ctaaattgag aaaaccctaa attgaataga tattcttcac tcttttctac
    11401 tattcttaaa ttttcatctg ttatctcaac tccataaggt atcctacttg caagacatgc
    11461 aaatgatggt ttattaaaag tatttaatcc taatgttttt gaaagcgacc taatttcttc
    11521 tttttttaaa ctactatctt gtaatggact aatcactcct aattcactca atgccttaag
    11581 cccaggtcta tagtctccta aatcatccat atttgtccca tctactatgt actttatatt
    11641 gtgttctttt gctacctctt ttattttaga gaatatatat tttttacaat gatagcatct
    11701 atcaacacca ttttctttaa attcttttaa atcaaagttt tctatattta atatgatatg
    11761 tttaacacca aaattttgag tatattgttt agcctcttca atttccctac ttgaatgcat
    11821 cattgcgtga atagtcacag ctataacatt ttcacctaat gtatcttttg cgaccttaag
    11881 caaaaaatta ctatctactc caccagaata agcaactaca acactgccta attcaagtaa
    11941 tctttttttt aatttatcta atttttcttt ttccttcaca aaacttacct ccatatctgg
    12001 aaaaatatga actataaaac tatatattat tttttacatt cttaatgatt ataacatata
    12061 aagtatttat tttata
//
LOCUS       DAEPXF010000114        11863 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain HB27
            SAMN13376389-rid12479423.denovo.120, whole genome shotgun sequence.
ACCESSION   DAEPXF010000114 DAEPXF010000000
VERSION     DAEPXF010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13376389
            Sequence Read Archive: SRR10518432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 11863)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11863)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001069624.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001069624.1
            Genome Coverage       :: 315x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/15/2021 01:54:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,165
            CDSs (total)                      :: 4,076
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 89
            rRNAs                             :: 10, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 10 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 19 of 69
            Pseudo Genes (incomplete)         :: 44 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 10 of 69
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11863
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13376389-rid12479423.denovo.120"
                     /strain="HB27"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="China: Hebei"
                     /collection_date="2017"
     gene            128..1441
                     /locus_tag="KRL67_002641"
     CDS             128..1441
                     /locus_tag="KRL67_002641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBH1523507.1"
                     /translation="MHTQLQKTIGLSAALSTVVGMVIGSGVFFKPQAIYTTTNGAPGL
                     GIIAWLLGGFITITAGLTATEISAAIPKTGGMMIYIEEIYGEKLGFLTGWMQTVLFFP
                     GTSAALGVIFAQQASELLGMSPNNMANVLPIAIGVILFLALLNIIGSSLGGKVQTVAT
                     IGKMIPLILIIVFGFIKGQSSEVLNPFVGDGVNASNALGQVLIATLFAYDGWINVGAI
                     SGEMKSPEKDLPRAIVGGLSLVMAVYIIINIAYLWVVPASELATVTSPATLVATRLFG
                     NIGGKVITVGILISVFGTLNGYLLTGSRIPYTLAEMGTLPASKTLLKVNSGGSPVNSI
                     LLITVLACVYALSGQFNLLTDLTIFSIWVFYVLTFIGVMRLRRQRPDLHRPYKVPLYP
                     IIPIIAILGGLFVIINQILTSTIISLGGIFITLLGLPVYYYMKKR"
     gene            1613..2788
                     /locus_tag="KRL67_002642"
     CDS             1613..2788
                     /locus_tag="KRL67_002642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HBH1523508.1"
                     /translation="MLGLYVHIPFCVKKCKYCDFNSYKMDIDSKKRYIEDLKIEMKLY
                     SNKLYKDNKYKNKECCSLNKNDKITSIFVGGGTPSILTSDEIREVFISIKEMFDIDEN
                     AEITIECNPGTLTLEKLKTMKEIGINRLSIGLQAIQEKHLNFIGRIHTYEEFEKNYKD
                     ALSVGFKNINVDLMYSLPNQTLCDWKETLEKVVHLNPTHISAYSLILEEGTELYNMYE
                     SNKFELIDENVDIEMYEYTINYLKSKGYNQYEISNYSKEGYNCEHNILYWECEHYIGI
                     GAGASGYINENRYNNVESLEDYHLSLVKREKPIQENEILSEKDMIEEKIFMGLRMNKG
                     IKFEDFKKKFGIDFREKYNKQIEMLLARKLINQSFEGIQLTQKGREISNSVFIEFME"
     gene            2891..3910
                     /gene="hrcA"
                     /locus_tag="KRL67_002643"
     CDS             2891..3910
                     /gene="hrcA"
                     /locus_tag="KRL67_002643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcription repressor HrcA"
                     /protein_id="HBH1523509.1"
                     /translation="MELNERKLNILKAIVKDYIETAEAIGSRTISKRHDLGVSAATIR
                     NEMADLEELGYLIQPHTSAGRVPSEKGYKLYVNSLMSKSELDDNDKILIEQCMNHNIN
                     HIKELIHETSKLLSQLTNYTTVAVTKSLINQSVIKHIQLVAMNDNNILLIVVTDKGDL
                     KKANLTTNVYLDQSKLNLISDNLTRKLLGKSITDLDDNLIAFIKYEISEYSGLIDELL
                     NALNSNMKEEDFSLSLNGATNIFSYPEFNDVLKAKSFLNMLEKKETIADIIKSKGIQK
                     DNLNIIIGSDNDCELAQDCSIVTATYNVDRDLVGRISFIGPTRMDYARIYSIINYMSL
                     LINRK"
     gene            3940..4560
                     /gene="grpE"
                     /locus_tag="KRL67_002644"
     CDS             3940..4560
                     /gene="grpE"
                     /locus_tag="KRL67_002644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HBH1523510.1"
                     /translation="MDKKNEQQEVREENDTSINQESETQVELEEEVVNEECETSSEKT
                     DEKEVDDENVTDINSKLAEKKLQDELDELNDKYQRLQAEYANYRRRTQQEKETIGVFA
                     NEKIITELIPVIDSMERALDACENKEDTMYKGISLVHKQLIDTLVKFGVEEIEAESKE
                     FDPNLHLAVMQESIDGVEANQIVMVLQKGYKLGTKVVRPSMVKVSC"
     gene            4652..6499
                     /gene="dnaK"
                     /locus_tag="KRL67_002645"
     CDS             4652..6499
                     /gene="dnaK"
                     /locus_tag="KRL67_002645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000034699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HBH1523511.1"
                     /translation="MGKIIGIDLGTTNSCVAVLEGGEAQIIANSEGMRTTPSVVAFTK
                     DGERIVGEPAKRQAVTNADKTITSIKTHMGTDYKVNIDGKSYTPQEISAIILQKLKSD
                     AESYLGQTVTEAVITVPAYFTDAQRQATKDAGRIAGLDVKRIINEPTAAALAYGMDKL
                     DQEKKILVFDLGGGTFDVSILEIGDGTFEVLATAGNNRLGGDDFDQIVIDYLAEEFKK
                     AEGVDLRNDKMALQRLKEAAEKAKKELSSTMSSNINLPFITATAEGPKHLNIDLSRAK
                     FEELTRGLVEKTMEPTKTALQDAGLSTGDIDDVLLVGGSTRIPAVQEAVKKFIGKEPH
                     KGINPDECVAAGASIQAGVLAGDVKDLLLLDVTPLSLGIETMGNVMTKIIERNTTIPT
                     KKSQIFSTAADNQTAVDIHVLQGERSMAYDNTTLGRFQLTDIPPAQRGIPQIEVTFDI
                     DANGIVNVSAKDLGTGKEQKITITSNTNLSEAEIEQKIKEAEMNAEADKQKKEKIEAF
                     NQAESTIYQTEKTLNELGDKISSGEKEDIEKAIADLKAVKDNQDATAEELKKATDEVM
                     TKFQKVSQEMYQKAAQEQQAAQGAEQAQDNGPKDDNVVDADFKEVDEDK"
     gene            6710..7864
                     /gene="dnaJ"
                     /locus_tag="KRL67_002646"
     CDS             6710..7864
                     /gene="dnaJ"
                     /locus_tag="KRL67_002646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HBH1523512.1"
                     /translation="MSTKRDYYEVLGISKGAEAQEIKKAYRKLAMKYHPDRNPGDKEA
                     EEKFKEINEAYEVLSDDTKRKTYDQFGHDGLNGQGGFGGQGGFGGQGFGGFEDMFGDI
                     FGDMFGGSFGGGRTRRRGPQRGADIRQSVTISFEEAAFGKKMSIKVNRSEECEECNGT
                     GAKPGTSKKTCSTCNGTGQVRTVQRTPFGNIASSRPCSACNGTGEVIESPCSKCHGTG
                     NTRKVKTIEVDIPAGIDDGQMIKLSGQGEVGEKGAPRGDLYIVVNVKSHPLFTRDGND
                     IYFEMPITFVQATLGDEIEVPTLDGKVKYSVPEGTQTGTVFRLKEKGIPRIRGNSRGD
                     QYVKVVVEIPKKLNDKQKELLREFAKECGSNVHEKKKTFGQKIEDMFKKK"
     gene            8829..9764
                     /locus_tag="KRL67_002647"
     CDS             8829..9764
                     /locus_tag="KRL67_002647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HBH1523513.1"
                     /translation="MYYIGVDIGGTGIQAGVVDNYGKIIFRSECKTVIEKGFEGILND
                     IKIMIYKLLEDNKLTMSDIKSIGFGVPGFINKEGLVTCVNLKWNKKAFNKELKRRFPD
                     VEIHGENDATVAALGEAKFGSMKGANVGVLYTLGTGVGGGIVINQKVFSGAHGLGSEI
                     GHQIIGENYFNCNCGNNGCVETFCSATAIIKYSQKLIEEGEKSRILDLAEGNLENVNA
                     KMVFDAYRENDLVAIKVINRFKEYLAKTFANTINSLDPEIISIGGGISKSSDIILDGI
                     EDLVRKFVLYKTEDIATITCATLGSDAGIIGAAFL"
     gene            9800..11173
                     /locus_tag="KRL67_002648"
     CDS             9800..11173
                     /locus_tag="KRL67_002648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBH1523514.1"
                     /translation="MGKTNARRTLFLIAIGSGTMLNPLNSSMISLALHSIQNDFHISF
                     TTVSWIVSAFYLASAIAQPVSGKIGDLIGRKVLFLSGLMLVILSASIVPLVQSFFILI
                     FIRVIQAIGTSTLYPSGVALIQNNIKERQSSALAVLTIFASTTAGLGPTLGGLLLDLG
                     GWHAIFLVNIPVVLVSICLGYFLFPKEVKEKKSIKETLKNVLSRLDIIGILLFSIAMI
                     FILLFLLSMKESFNLEQLVFGIILMCLFIWHELRTKLPFIDIKLFVSNPKLSKVYLQF
                     IILNFFNYILFFGLPSYFQDALHYSAKSTGLFMLFMSGIGIFISPLTGRWIDKSGTRF
                     PLVTSSIFMFISAVLLSVVFIHIPVIGKGIILSLAGISYGVGNVALQSSMFEESPRDS
                     IGTASGLFQTSRYLGSILATVLLGMVFEVNITSEQFQILGYVMVVLGTISFLLNFILK
                     KELRKVE"
     gene            complement(11361..>11863)
                     /locus_tag="KRL67_002649"
     CDS             complement(11361..>11863)
                     /locus_tag="KRL67_002649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBH1523515.1"
                     /translation="FIVNSKYNLDINVTTQNIIETETYQSTYIFYLGKLDSKADFDFR
                     LQLCSDLLPHIIKDNAENKFLNLFDLIRYKTLDLINEDNILNKLIDFNKIKSIDEELL
                     YTGLKFINNDLNISKTSTSMFLHRNTLVYRLEKINEILGFDLKNFENAMIFYLSVKSY
                     FLYKKI"
BASE COUNT         4659 a         1298 c         2174 g         3732 t
ORIGIN      
        1 gatttttaaa cgcatagcat ttactctagg actatagact aatataaaaa aaaactttgg
       61 gaatactaaa accataaatt tataaaatta atattttata tactgtaaaa cagtgaggag
      121 ggacagtatg catactcaac tacaaaagac tattggtctt tcagcagctc tttctactgt
      181 tgtaggtatg gttataggtt cgggagtttt ttttaagcca caagcaatat acactacaac
      241 taatggagct ccaggtctag gtattatagc gtggctttta ggtggtttta taacaataac
      301 tgctgggctt acagctacag aaatttcagc tgctatacca aaaactggtg gtatgatgat
      361 ttatatcgaa gaaatatatg gagaaaagtt aggcttttta actgggtgga tgcaaactgt
      421 attatttttc ccaggtacat cagcagcatt aggagtaata tttgctcaac aagcatctga
      481 acttttagga atgagcccaa ataatatggc aaatgtactt cctatagcaa ttggagttat
      541 actattttta gcattattaa atataatagg ttcatcttta ggaggaaaag ttcagacagt
      601 agccactatt ggaaagatga tacctttaat tttaataata gtctttggat ttattaaagg
      661 tcaaagtagt gaagtattaa atccatttgt aggagatggt gtaaatgcat caaatgcact
      721 tggacaagta ttaatagcta ctttgtttgc gtatgatggt tggataaatg ttggggctat
      781 atcaggagaa atgaagtctc cagagaaaga tttaccaaga gcaatagttg gtggtttatc
      841 attagttatg gcagtttata ttataataaa tatagcttat ctttgggtag taccagcaag
      901 tgaattagca acagttactt ctccagctac attagtagca acaagattgt ttggaaatat
      961 aggtggaaaa gtaataactg tgggtatatt aatatcagta tttgggacat taaatgggta
     1021 tttattaacg ggttcaagga taccttatac attagctgaa atgggaactt taccagcatc
     1081 taaaactctt ttaaaagtaa attctggagg ttctcctgta aattcaatac tacttataac
     1141 tgtgttagcc tgtgtatatg cattatctgg tcaatttaat cttttgacag acttaactat
     1201 attttcaata tgggtatttt atgtattgac ttttataggt gttatgaggc ttagaagaca
     1261 aaggccagat ttacacagac catataaagt ccctttatat ccaataatac caattatagc
     1321 tatactaggt ggactgtttg taataataaa tcaaatattg acttcaacaa tcatatcttt
     1381 aggcggaata tttataacac tattaggttt accagtttat tattatatga aaaaaagata
     1441 actacttaat ttaagttaga taaatttggg taaaggtgaa aattaacttt tatccaaatt
     1501 ttttatactg ttatacaata taagaattat atattacttg atgtgttatt ttgtttgatt
     1561 tataatttag taattgatgc tttaagataa tttaagaaat gaggaataaa aaatgctagg
     1621 actttatgta catattccat tttgtgtgaa aaaatgtaag tattgtgatt ttaattctta
     1681 taaaatggac atagattcaa aaaaaagata tatagaagac ttaaaaatag aaatgaaatt
     1741 atatagtaat aaattatata aagataataa atataaaaat aaagaatgct gtagtctaaa
     1801 taaaaatgac aaaataacga gtatatttgt agggggaggt acacctagta tactaacatc
     1861 tgatgaaata agagaagtat ttataagtat aaaagaaatg tttgatatag atgaaaatgc
     1921 agaaataact atagagtgta atcctgggac attaacatta gaaaaattaa aaactatgaa
     1981 agagattggc ataaatagac ttagtatagg gctacaagcc attcaagaaa agcatttgaa
     2041 ttttatagga agaatacata cttatgaaga atttgagaaa aactataaag atgcattaag
     2101 tgtaggtttt aaaaatatta atgtagattt aatgtacagt cttccaaatc aaactctatg
     2161 tgactggaag gaaactcttg aaaaagttgt acacttaaat ccaacacata tatcagcgta
     2221 ttcccttata ctagaagaag gaactgaact atataatatg tatgaaagta ataaatttga
     2281 gcttatagat gaaaatgtag atatagaaat gtatgagtat acaataaact acttaaagtc
     2341 aaaaggatat aatcaatatg aaatatcaaa ttactcaaaa gaaggctata attgtgagca
     2401 taatatttta tattgggaat gtgaacatta tatagggatt ggagcaggag cttctggcta
     2461 tattaatgag aacagataca ataatgtaga atctttagaa gattatcatt taagtttggt
     2521 aaaaagagaa aaaccaatac aagaaaatga aattctttct gagaaagata tgatagaaga
     2581 aaagatattt atggggctta gaatgaataa aggcataaaa tttgaagatt ttaaaaaaaa
     2641 atttggaata gattttagag agaagtataa taagcaaata gaaatgctat tagctagaaa
     2701 gcttataaat cagagctttg aaggtataca attaactcaa aaaggaagag aaatatctaa
     2761 tagtgtattt atagaattta tggaataatt tttaaggtgt tgacaaaata aatttataat
     2821 ggtatataat atatataatc tttttagcac tcgaacttag tgagtgctaa cagctgggag
     2881 gtataataat atggaattaa atgaaagaaa attaaatatc ctcaaagcta tagttaaaga
     2941 ttatatagaa acagctgaag ctataggttc tagaacaata tctaaaagac atgatttagg
     3001 tgttagtgct gctaccataa gaaatgaaat ggctgacctt gaagaattag ggtatttaat
     3061 tcagcctcat acttctgcgg gtagagttcc atctgaaaaa ggctataagt tatatgtaaa
     3121 ctctcttatg agtaaaagcg aattggatga taatgataag attcttattg aacaatgtat
     3181 gaatcataat ataaatcata taaaagaatt gattcatgaa acttctaagc tattatcgca
     3241 actaacaaac tatacaactg tagcagttac taagagttta ataaatcaaa gtgttataaa
     3301 gcacatacag ttggttgcta tgaatgacaa taatatttta cttatagttg taacagataa
     3361 aggggactta aaaaaagcta accttacaac taatgtatat ttagatcaat ctaagttaaa
     3421 tttgatttct gataatctta caaggaagct cttaggtaaa agtataacag atttggatga
     3481 taatctaatt gcttttatta aatacgaaat aagtgagtat tctgggctta tagatgagct
     3541 tttaaatgct ttgaactcaa atatgaaaga agaagatttt tctctatcat taaatggagc
     3601 tactaacata tttagttatc cagaatttaa tgatgtatta aaagcaaagt catttttaaa
     3661 tatgttggaa aaaaaggaaa caatagctga cataattaaa tcaaagggaa tacaaaaaga
     3721 caatctaaat ataataattg gtagtgataa tgattgtgaa ctggcacagg attgtagtat
     3781 agttacagca acttataatg tagatagaga cttggttggg cgaataagct ttataggccc
     3841 taccagaatg gattatgcta gaatttactc tataataaat tatatgagtt tattaattaa
     3901 tagaaagtaa agaaatttta aattgcgagg tgtaaagtct tggataaaaa aaatgagcaa
     3961 caggaagtaa gagaagaaaa tgatacttct attaaccaag aatcggaaac tcaagtagag
     4021 ttagaagagg aagttgttaa tgaagaatgt gaaacatcaa gtgaaaaaac tgatgaaaaa
     4081 gaagttgatg atgaaaatgt aacagatata aattctaagt tagcagaaaa aaaattgcag
     4141 gatgagttgg atgaattaaa tgataaatat caaagacttc aagccgaata tgcaaactac
     4201 agaagaagaa ctcaacaaga gaaagagaca ataggcgtat ttgcaaatga aaaaataata
     4261 actgagttga taccagttat agatagtatg gaaagagcct tagatgcatg tgaaaataag
     4321 gaagatacta tgtataaggg tataagcctt gtacataaac aattaataga cactttggta
     4381 aaatttggag tagaagaaat agaagcagaa tctaaggaat ttgaccctaa tctacatcta
     4441 gcagttatgc aagaaagtat agatggagtt gaagcaaatc aaatagtaat ggtacttcaa
     4501 aaaggatata agctaggaac taaagttgta agaccttcaa tggttaaagt ttcttgctaa
     4561 gtataaatta aaggttacat tatttaacct aatataaaat attaaaataa gtaaaactta
     4621 attatataat tactcgagga ggattttagt catgggaaaa ataataggaa tagatttagg
     4681 aacaacaaat tcatgtgttg cagtgttaga aggtggagaa gcacaaataa tagcaaatag
     4741 tgaaggtatg agaactactc catcagtagt agcatttaca aaagatggtg aaagaatagt
     4801 tggagaacct gcaaaaagac aagcagttac aaatgcagat aaaacaataa cttctataaa
     4861 aactcatatg ggaactgatt ataaagtaaa tatagatgga aaatcatata ctccacaaga
     4921 aatatcagca ataatattac aaaaattaaa atcagatgca gaaagttact tgggacaaac
     4981 agtaacagaa gcagttataa cagtaccagc ttactttaca gatgctcaaa gacaagcaac
     5041 taaagatgct ggtagaatag cagggcttga tgttaagaga ataataaatg agccaacagc
     5101 agcagcactt gcttatggta tggataaatt agaccaagaa aagaaaatat tagtatttga
     5161 cttaggtgga ggaacttttg acgtatctat acttgaaata ggagatggaa cttttgaagt
     5221 tttagctaca gctggtaaca atagattagg tggagatgac tttgaccaaa tagtaataga
     5281 ttatttagca gaggaattta aaaaagctga aggtgtagat ttaagaaatg ataaaatggc
     5341 tcttcaaaga ttaaaagaag cagcagaaaa ggctaagaaa gaattatcat caacaatgag
     5401 ctcaaacata aacttacctt tcataactgc tacagcagaa ggaccaaaac acttaaatat
     5461 agatttatca agagctaaat ttgaagaatt aactagaggt ttagttgaaa aaactatgga
     5521 accaactaag acagctttac aagatgcagg actttctaca ggtgatatag atgatgtatt
     5581 attagtaggt ggttctacaa gaataccagc agtacaagaa gctgttaaga agtttatagg
     5641 aaaagagcct cacaaaggta taaacccaga tgagtgtgtt gcagcaggtg cttctataca
     5701 agctggagtt ttagcagggg atgttaaaga cttattatta ctagatgtta ctccattatc
     5761 tcttggtata gaaactatgg gtaatgtaat gactaagata atagaaagaa atactacaat
     5821 accaactaag aagtctcaaa tattctcaac tgctgctgat aatcaaacag ctgttgatat
     5881 acacgttctt caaggtgaaa gaagtatggc ttatgacaat acaactttag gaagattcca
     5941 attaacagat ataccaccag cacaaagagg aatacctcaa atagaagtta cttttgatat
     6001 agatgcaaat ggtatagtta atgtttcagc taaagattta ggtacaggaa aagagcaaaa
     6061 aataactata acatcaaaca ctaatttatc agaagcagaa atagagcaaa agataaaaga
     6121 agcagaaatg aatgcagaag ctgacaaaca aaagaaagaa aaaatagaag cattcaatca
     6181 agctgaatct actatatatc aaactgaaaa aactttaaat gaacttggag ataaaataag
     6241 ttcaggtgaa aaagaggata tagaaaaagc tatagcagac ttaaaagctg taaaagataa
     6301 tcaagatgct acagcagaag aacttaagaa agctacagat gaagtaatga ctaagttcca
     6361 aaaagtatct caagaaatgt accaaaaagc agctcaagaa caacaagcag cacaaggtgc
     6421 agaacaagca caagacaatg gacctaaaga tgacaatgta gtagatgctg actttaaaga
     6481 agtggatgaa gataaataag ctatataata tgtaacacta aatgataatt ggaacatata
     6541 tgatttgtga aaatcatata gatgttaaag atatatggac aatacacatc ataaagaata
     6601 taatatatca tagattatat aacttaaagt agaatataag aattaatgat agcttgtagt
     6661 ttattattct acaagctatc gttttgtaaa atgaaggtgg tgtcaatttt tgagtacaaa
     6721 aagggattat tatgaagtct taggtatcag taaaggtgca gaagctcaag agataaaaaa
     6781 agcttataga aaattagcaa tgaagtacca tccagataga aatcctggag ataaagaggc
     6841 ggaagaaaaa tttaaagaga taaatgaagc ttatgaagtt ctatctgatg atacaaagag
     6901 aaaaacttat gaccagtttg gacatgatgg gctaaatggt caaggcggtt ttggtggtca
     6961 aggaggattt ggcggtcaag gctttggtgg ttttgaagat atgttcggag atatatttgg
     7021 tgacatgttt ggaggaagct ttggaggtgg aagaacaaga agaagagggc ctcaacgtgg
     7081 tgcagatata agacagagtg ttactatatc ttttgaagaa gctgcatttg gtaaaaagat
     7141 gtctataaaa gtaaatagaa gtgaagagtg tgaagaatgt aatggaactg gagcaaaacc
     7201 aggaacatca aagaaaactt gttcaacttg taatggaact ggtcaagtta gaactgttca
     7261 aagaactcca tttggtaata tagcaagttc aagaccatgt agtgcttgta atggaactgg
     7321 agaagtgatt gaatctccat gtagtaaatg tcatggaact ggaaatacaa gaaaagttaa
     7381 gactatagaa gtagatatac ctgctggtat agatgatggt caaatgataa aactttctgg
     7441 tcaaggtgaa gtaggagaaa aaggtgctcc aagaggagac ttatatattg tagttaatgt
     7501 taagtcacat ccactattta ctagagatgg aaatgatatt tattttgaaa tgccaattac
     7561 atttgttcaa gcaactttag gtgatgagat agaagtacct actttggatg gaaaagttaa
     7621 atatagtgta ccagagggga cacaaactgg aactgtattt agacttaaag agaaaggaat
     7681 ccctagaatt agaggtaatt caagaggaga ccaatatgtt aaggttgtag ttgaaattcc
     7741 taaaaaatta aacgataagc aaaaagaact attgagagaa tttgcgaagg agtgtgggtc
     7801 gaacgtccac gaaaagaaga aaaccttcgg acaaaagata gaagatatgt ttaagaaaaa
     7861 gtagttaaag tagccaatat ataatcatat ttttgaaatc ttagttattt ataagattgt
     7921 caaaggagat attatgattt ttatattggc tatttttgta taaaaaataa atatgcataa
     7981 aataaaactg aatataaata attttctgaa aaatacttgt cctaaaatat ataatatgct
     8041 ataatataat caaataaaag aaaggggtga ttccagtggg aacggatgtg gcactgccaa
     8101 atgacgaaga aaactttatg gtaatctaca aaataaaatt agatactttt agtgctagat
     8161 taagtactgg aaaaactcat gagctgggat aattattgta attacctaga ctcaaacttt
     8221 tagtagtaaa atagtaatat tatatagaca aggtatctaa tttttaaatc ttttgtatga
     8281 attaaaattt ttataaaggg tatatctgat tagaatgtaa ttagggaaac ctagttgtga
     8341 attactttta gtatatacat gtgtaaagaa atatgtatat ggtattggtt attgtgctag
     8401 atgtgtatgt aaaggaatat gtgtctagtt gggtatatgt aaagaaatat acttataatc
     8461 tatgaaaaat ttttagtata aattgctaaa acattgagat tgttttttat agaaagttag
     8521 tttataccaa ctaaaaatgt gcttaagatt tgtttgatgt ttaattataa ataaggttgt
     8581 agataaaaaa ataagtagag acaaaggttt gaaatacttg atttaaatat agtttgtttt
     8641 gaacacttgc caaattaaaa taattaataa aatagcatct tctcatagta aaatatactg
     8701 tgagaggatg tttttgcttt gttcataaac ttttatgaaa aatttgcata gtgtcacatt
     8761 ttaatatact atatatataa taaaatatag ataaaattaa atgattcaaa agagaaaggt
     8821 gaaaagagat gtattatata ggtgtagaca taggaggaac aggtatacaa gcaggagtag
     8881 ttgataacta tgggaagata atttttaggt cagagtgtaa aacagtaatt gaaaaaggat
     8941 ttgaaggaat acttaatgac ataaaaataa tgatttataa gttgttggaa gataataaac
     9001 ttactatgag tgacataaaa tctattggat ttggtgttcc aggttttata aataaagaag
     9061 gattagtaac ttgtgtaaat ttaaaatgga ataaaaaagc ttttaataaa gagttaaaaa
     9121 gaagatttcc agatgttgaa attcatggag aaaatgatgc aacagtggca gcacttggag
     9181 aagcaaagtt tggaagtatg aaaggtgcta atgttggagt attatatact ttaggaactg
     9241 gtgtaggtgg aggaatagta attaatcaaa aagtcttttc tggagcacat ggattgggtt
     9301 cagaaatagg tcatcaaatt ataggagaaa actattttaa ttgtaattgt ggaaataatg
     9361 gttgtgtaga aactttttgt tcagcaactg caattataaa gtattctcaa aagctgatag
     9421 aagaaggaga aaagagtagg attttagatt tggcagaggg aaatttagaa aatgtaaatg
     9481 caaagatggt gtttgatgca tatagagaaa atgatttagt ggcaattaaa gtaataaata
     9541 gatttaaaga atatttagca aaaacttttg caaacacaat aaattcttta gacccagaaa
     9601 ttatatctat aggaggaggt atttctaaat ctagcgatat aatacttgat ggaatagaag
     9661 atttggtaag aaaatttgta ttatacaaaa cagaagatat agctacaatt acatgtgcaa
     9721 ctttaggctc agacgcagga attataggag cagctttttt ataatgtaaa tgaactgctt
     9781 attggaggcg tgatgatata tgggtaaaac aaatgctaga agaacgcttt ttttgatagc
     9841 aataggttca ggaactatgc tgaatcctct taattcttct atgatttcac ttgctttaca
     9901 tagtatacaa aatgattttc atattagttt tacaactgta tcttggattg tttcagcatt
     9961 ttacctagct agtgctattg cacaaccagt ttcaggtaag ataggagatt taataggtag
    10021 aaaggtctta tttttgagcg gtttgatgct tgtaatttta tcagcatcca tagtaccact
    10081 tgttcaatcg ttctttatat tgatttttat aagagtaatt caggcgattg gaacaagtac
    10141 actatatcca tctggggttg ctcttataca aaataatata aaggaaagac aatcttctgc
    10201 actagcagtt ttaaccattt ttgcttcaac tacagcagga ttaggtccga cacttggagg
    10261 attattgttg gatttaggag gatggcatgc tatatttctt gtaaatatac ctgttgtttt
    10321 ggtttctata tgcttagggt attttttatt cccaaaagaa gtaaaagaaa agaaatctat
    10381 aaaagaaact ctgaaaaatg ttttgtcaag attagatatt attggcatac ttctattttc
    10441 aattgcaatg atttttatat tattattttt gttatctatg aaggagtctt tcaacttaga
    10501 acaacttgta tttggtataa tactgatgtg tctttttatt tggcatgaat tgagaacaaa
    10561 attaccattt atagatatta agctttttgt gagtaaccct aaattgtcaa aagtttactt
    10621 gcaatttatt atattaaatt tctttaatta tattttgttt tttggtcttc ctagttattt
    10681 tcaagatgca ttacactata gtgcaaaatc gactggatta tttatgctat ttatgtctgg
    10741 tataggcata tttatatctc cattaactgg aagatggata gataaatctg gaactagatt
    10801 tcctcttgtt acatcatcaa tctttatgtt tattagtgca gtacttcttt cagtagtttt
    10861 tattcatata ccagtaatag gaaaaggtat catattatca ttagcaggta ttagttatgg
    10921 agttggtaat gtagccctgc aatcctctat gtttgaagaa agtcctcgtg acagtatagg
    10981 gacagcttct ggtctgtttc aaactagtcg ttatttaggt tctattttag caactgtttt
    11041 acttggaatg gtatttgaag taaatataac atcagaacaa tttcagatat taggttatgt
    11101 gatggtggtc ttaggtacaa taagtttttt attaaatttt atattaaaga aagaattaag
    11161 aaaagtagaa tagtggattt atggatttaa taagtctaat aactgataag tttaaaaata
    11221 ttgtttgtaa aatataacta gtttgatatt tattaattct aagttttgaa gcatagagta
    11281 ctcgataagt atacaatatt ttgaattaaa actaagcaga aatttgaact agtctttcaa
    11341 atctctgctt aatttagtgg ttaaattttt ttatagagaa aataagattt gacactcaga
    11401 taaaaaatca ttgcattttc aaaattcttt aaatcaaacc caagtatctc attaatcttt
    11461 tccaatctat atactagcgt gtttctatgt aaaaacatac tagttgatgt cttagaaata
    11521 tttaaatcat tatttataaa ttttaatcct gtatatagaa gttcttcatc aatagattta
    11581 atcttattaa aatctattaa tttattgagt atattgtcct cattaattaa atctaaagtt
    11641 ttatatctaa taaggtcaaa caaatttaaa aacttatttt cagcgttgtc ttttataata
    11701 tgaggtagta aatcagaaca taactgtaat ctaaaatcaa aatcagcttt actatctaat
    11761 ttaccaagat aaaaaatata agtactttga taagtttcag tttcaattat attttgtgta
    11821 gttacattaa tatctaagtt atatttagaa tttactataa aaa
//
LOCUS       DAEPXF010000115        11854 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain HB27
            SAMN13376389-rid12479423.denovo.121, whole genome shotgun sequence.
ACCESSION   DAEPXF010000115 DAEPXF010000000
VERSION     DAEPXF010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13376389
            Sequence Read Archive: SRR10518432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 11854)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11854)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001069624.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001069624.1
            Genome Coverage       :: 315x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/15/2021 01:54:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,165
            CDSs (total)                      :: 4,076
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 89
            rRNAs                             :: 10, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 10 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 19 of 69
            Pseudo Genes (incomplete)         :: 44 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 10 of 69
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11854
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13376389-rid12479423.denovo.121"
                     /strain="HB27"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="China: Hebei"
                     /collection_date="2017"
     gene            185..349
                     /locus_tag="KRL67_002650"
     CDS             185..349
                     /locus_tag="KRL67_002650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH1523516.1"
                     /translation="MENKKLQIEKITDKIEIEEVEVQADPCKNDPYNGIYDCLRDCSS
                     GTSPKGSPAY"
     gene            758..3301
                     /locus_tag="KRL67_002651"
     CDS             758..3301
                     /locus_tag="KRL67_002651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HBH1523517.1"
                     /translation="MKKQRGKTVALLSSIILSVMLIFSIIVIRASGYDSQIQEAKNLH
                     GEYQIWFEGIDINKAKKLKNDTEVKKSSSVEYFCEVINKASGVKLALNSFDKDFIESL
                     NYKMIGRNPTKEGEIVIEKEVAKQIGISNPLNKNIDLMLMNKYVDSDGTNKMDSANQT
                     FKIVGLIEKPNKYYTSLSGSLGGIKTQAFIYKGTKLPIRTEDTYKGVIFLKSEKNTSK
                     FISKMEKKLNLTWDYLHENTEVSAAKLLKDLSENKENIQKMFLLVIVSSLVIYNIFNI
                     ILQDMIVQIGLMRSIGMSNKKIKSMFAVLSLIYIVLGTIIGMLLGMILSYIGVRLIYG
                     YSSMMTVEISSIIYSFSVSILAVSISSFIVVRKAVKMSIINSIRSSEMYERKLKYSSN
                     KTNKTQGNIIRSLAIRNIGRNKSRTIITILAITMVGTMFIFNLGTKSLLKANIESGVT
                     GGGLGMSYGSVDKTVSGSYNGTESLFYKIDESMIEEIKNIEGVESVEPNFFNPNGYIL
                     LSKDKISKSYKEELDRRNLLFQDKNDNEYPLLIRGYSDELLKKRESFIEEGKNLINTK
                     NGQYKKVMLVNNTNSQVTHSFDAKVIENVKVGDVIDIKLPVYKDGTQKYETFKVEVSA
                     IMKEVYAAAQDGNVHASGAQVIFREDDYRELTGQKEYNKLYVMAQKGKLYPVEKKLEE
                     LTKNYSFTSIGGKGEDLKLVGVQQSSEERLAVIYQCLIILILAVNSIFIMRSNIISRR
                     KELSTLRAIGMSIKSIKKVLIIESELYGIIATIIGTIIATIYYNWGISKSNKTLLAGG
                     FNRTIEYNIPFNQIFILFAIFIIMGFVAVYLSKDKIEGTEITEGISQND"
     gene            3336..4613
                     /locus_tag="KRL67_002652"
     CDS             3336..4613
                     /locus_tag="KRL67_002652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HBH1523518.1"
                     /translation="MFDDIQIKFRENDLLINKRNGKVILYNKEKKRHVKLSEEVYEYT
                     RLAEKNNWKVRELIDCFKKDEDKKYIESILNIMVKNEVIDGNLFNKKNFKDMHLSLTN
                     RCNLSCNHCVSSCSPKEEDYMDTSMILKLIDNLCELNVRSLVLTGGEPLVREDFFEIV
                     NYIKKKLPLEELSLSTNATLINDTNIDFIISKFHKVDISLDGIDEETCSNIRGKGVFK
                     KVLNVVYELQNKKFNNISLSMVFGEKNYQMSEKFKKLNEDLNTKAIERYFLPSGRGLI
                     NRKIYSDENSVLPLVIPKFRENEINGKKNRINPCSCSCNACIEQVFIDHKGNIYPCPS
                     LVEKEYLVGSVFDKKIINNLKENELKENQAFRNLNEIYPFNFEKCKDCDVNIFCFKCP
                     AMVNKVKNDDKELNIWCNLTKRNMELAVWGGEV"
     gene            4614..5660
                     /locus_tag="KRL67_002653"
     CDS             4614..5660
                     /locus_tag="KRL67_002653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HBH1523519.1"
                     /translation="MKFTIFVTEKCNLRCTYCYERGMDRGKSMDINTANKTIDFICKN
                     IKNEELDIPLYIVFHGGEPLINFKLVKHIHKELSKRVLERKIIYDMTTNATYLDEEII
                     NFMCKNIDNISISIDGTKKFHDKNRIFSNGDGSYDLVIKNAKRFLDIGKDVRVRMTYT
                     TDTISELYESIAQIVRYGFTNIVPIADFMSLNWTNNHIDIINEQISKLINLKKEHPNI
                     DISLLDIKNLNIKKGDCFGGIVSFAIDVNGDIFPCTFSVGDNEFKLGSVFDRNISEEK
                     RQELIKIYISENEECKDCLRKEWCTSVRCKLMNRKVTGEFNKAIPIICALEKVILTTT
                     RKIKMEEKSKEAMI"
     gene            5724..6962
                     /locus_tag="KRL67_002654"
     CDS             5724..6962
                     /locus_tag="KRL67_002654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HBH1523520.1"
                     /translation="MEDRFFINDVCDNIINENQVILANKINGKWIKIPIECYKVIEYS
                     IKNKKPLDKLEDVFNLDEDKKYYKQVVDKLEQLGFVSNFIVDKFKLERVNLVSFSPTN
                     RCNLRCDYCCADSKIENKDYLGTEELKEAIDNIVKINPRILTITGGEPLLRDDFFEIL
                     SYTKEKFKGKIRLSTNATLIKENQVDEIIDGLYAMEISLDGYDELSCSKVRGKGVFTK
                     VINLIKYIKSKGFNNIGLSMVVGKNNENDIDKFNKINYELGTIPIIRNFMNIGRGNES
                     YTRYLDDELDIGYICEDDYINKNSLNSNMCKAGVNQISIDYKGDVYPCPNLEYDDLKM
                     FNILQFDESMLEKILNRNMEIFDKFDILKPMNMEECKDCKVNVFCTTCPSKMYTLKNN
                     KEMLNSNCKKMKSILEPIVW"
     gene            7043..8086
                     /locus_tag="KRL67_002655"
     CDS             7043..8086
                     /locus_tag="KRL67_002655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HBH1523521.1"
                     /translation="MEKIKVAVVDTGIDVNDDSIKKYIKFNKNIQLEEVVEYKDLDDT
                     HGHGTLCSKTILSVCRNTDRIEIYPIKIFNQGGCTSGERLLEALERNLDSDIDLINIS
                     ASTLNDKYKRELKYICEELCKKGKIIISSHHKRAKDCDSFPTVFNSVIGVTGSNDIYN
                     DNDYIYEKGKKIQIIANTKECFVEFRNEVTHFGKSSRASAVITGTICNIFNKYGKIMF
                     DELEKIFEKESLDKNINTVKSKFRNKQNNFDLCITKNQKLDISRRIVNMINKKFAVHK
                     IDLDFLDKYSIFNNFTDLGNHNAFKFLTEINKEFEIEISYKGLFLYELENLNDLVNLV
                     CKHLNKASVNNSI"
     gene            8184..8903
                     /locus_tag="KRL67_002656"
     CDS             8184..8903
                     /locus_tag="KRL67_002656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBH1523522.1"
                     /translation="MKTLKIKNLKKIYGKYENEVHAIAGIDLEIEPKKFTAIVGASGS
                     GKSTLLHCMAGLDKPTSGNVYLDDLDIYTLNDDKLSKIRREEFGFIFQSYNLIPVINV
                     YDNIVLPVLLDSKREDKTHINNLIFRLGLESQVKRFPNELSGGQQQRVAIARALANKP
                     SVIFADEPTGNLDSKTTAEVMELLEICVKEFSQTLVMITHSDKIAETADKIITISDGK
                     IVKILDKSFEEHNMGETEIKI"
     gene            9028..10881
                     /locus_tag="KRL67_002657"
     CDS             9028..10881
                     /locus_tag="KRL67_002657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBH1523523.1"
                     /translation="MDEMKKKCSKNKKIENRNNKNKSLKRFAFLTFKKNKGKLLLSFI
                     MVIIVAVVDLVQPQVTRMIVDEGIGTKDANLLLKLMFIYGAAGVSSAILYLLLSYLYS
                     AIKKNVTVDIKIKLLNHLSKLSGKYYSNIKTGNIISIIESDIFIIENFGAELAFSILV
                     NIFTASMALFFLVRMQVDLFLIVLILQIILSIFQNKFTKLISKNTREIREDSGSLSNK
                     IQEYVSNIKNVVVSKSRMKFFSSYLSEEKNLIRKYLKLDLIMNGNIAIAKSISILVTV
                     SIYGVGGYKIISGNMTIGELLAFQQYTNMFIGPCISIVRANTRIQQAKASLDRVYSVI
                     DEEIDIKVNNRGLRIEDKECNIIEFKNIYFSYKEIGKKKLQNLEQDEQIDCTYEDIET
                     DSYILSNINITFRKGETTALIGESGSGKSTIVNLLYRLWDIQKGEITIDGINIKNINL
                     KSLRKSINIVTQDMLMFDDTIRNNININKSLSDEELKNICSVVGMNDFISRLENGFDT
                     IIGEKGVKISGGQKQRIALARSLVNDSKILILDEATSALDNISQSEILRNIRAYVKGK
                     IVIIIAHRLSTIKDADNIYVLEKGKVVESGKDKELFIRNGIYRKLVTLNTQ"
     gene            complement(11648..>11854)
                     /locus_tag="KRL67_002658"
     CDS             complement(11648..>11854)
                     /locus_tag="KRL67_002658"
                     /inference="COORDINATES: protein motif:HMM:NF024729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HBH1523524.1"
                     /translation="MSRRGNCWDNAPIESFFSHFKSELIYLIDTTDPKKMISLINDYI
                     YFYNNERIQLKNGMSPIEYRTHCA"
BASE COUNT         4966 a         1102 c         1994 g         3792 t
ORIGIN      
        1 ttaaatctgt aattacagag caattgttag aattgtaatc tgttttacat atagaaaata
       61 tttatttata tattttatcg ataggataag gcgccgtgta taaaaaatat tttcaaacaa
      121 attaaattga tatgtagctt gtgtaagtta aaaagtacaa agttacagaa aggtaggaat
      181 aattatggaa aataaaaaat tacaaatcga aaaaatcact gacaaaatag agatagaaga
      241 ggtagaggtg caagcagatc catgtaaaaa tgatccatat aacggaatat atgattgctt
      301 aagagattgt agctcaggaa catcaccaaa aggctcacca gcatattaag ttttactatg
      361 aaagtttaga ttattgacaa caataggcca cctgctatgc gggtggcatt cttggttata
      421 cgaaaattaa tcatattcta atataaacta tttaaggatt taacttaata aagttattta
      481 aaaattatgc aactataaag gtatagatat aatagaataa catataaatt tagattatac
      541 atgttagcca aagtctcaat ttaaaatttt atgtagtact tgaactgcac caaacttaaa
      601 gtgtaaatag gataatagaa agttttataa ataagaatat tatgtaatta agcaatgagt
      661 tatgtcccca aaatagaaaa tttgttttat aagggatgat aaatttttgt catggaggtg
      721 agaagttttg tttatagtaa aacttgcaat ttcttatatg aaaaaacaaa gaggaaaaac
      781 agtagcacta ttatcgagta ttatactttc agtcatgctt attttctcaa ttattgtaat
      841 tagagcctcg ggttatgatt ctcaaataca agaagcaaag aacttacatg gtgaatatca
      901 aatatggttt gaaggaattg atataaataa agcaaaaaag ttgaaaaatg atactgaagt
      961 aaaaaaatca agcagtgttg aatatttttg tgaagtaatt aataaggcaa gtggtgttaa
     1021 acttgcttta aattcatttg ataaagattt tatagagtca cttaattata agatgatagg
     1081 aagaaatcca actaaagaag gcgaaatagt catagaaaaa gaggttgcaa aacagatagg
     1141 tataagtaat ccattaaaca agaatattga tttaatgtta atgaataagt atgttgatag
     1201 cgatggaact aacaaaatgg atagtgccaa tcaaactttt aaaatagttg gtttaattga
     1261 aaagccaaat aaatactata cctctctatc cggttcacta ggaggaatta aaactcaggc
     1321 ttttatatat aaaggcacaa aacttccaat aagaactgaa gatacatata aaggtgtaat
     1381 attccttaaa tctgaaaaaa atacatctaa atttatcagt aaaatggaaa agaaactaaa
     1441 tcttacttgg gattacttac atgaaaatac agaagttagt gcagcaaaac tattaaaaga
     1501 tttatctgaa aataaggaaa atattcaaaa gatgtttctt ttagttattg tatctagtct
     1561 tgtaatctac aatattttta atattattct gcaagatatg atagttcaaa taggacttat
     1621 gagatcaatt ggtatgtcaa ataagaaaat caagagtatg tttgcagtac ttagtcttat
     1681 ttatatagtt ttaggaacga taataggtat gttacttgga atgattttat catacattgg
     1741 agtgagatta atctatggat atagttctat gatgactgta gagatttcaa gtataattta
     1801 ttctttttca gtttctatat tagcagtgtc aataagtagt tttatagtag tcagaaaagc
     1861 tgtgaagatg tctataatta attcaataag aagtagtgaa atgtatgaaa gaaaattaaa
     1921 atatagtagt aataaaacaa acaaaacaca gggtaacata attagaagtc ttgcaattag
     1981 aaatataggg agaaataaat ctagaacaat tataacaata cttgcgatta ccatggttgg
     2041 aacaatgttt atatttaacc ttggcacaaa aagtctatta aaagccaata tagaatcagg
     2101 agtaactggt ggtggacttg gcatgtcata tggaagtgta gataaaaccg taagcggtag
     2161 ttacaatggt acagaaagct tattttacaa aattgatgaa agtatgatag aagagataaa
     2221 aaatatagaa ggggtagaaa gtgtagaacc aaactttttt aatcctaatg gatatatttt
     2281 actatctaag gataaaatat caaaatctta taaagaggaa ctagatagaa gaaatttact
     2341 atttcaagac aaaaatgata atgaatatcc acttcttata agaggttata gcgacgaatt
     2401 attaaagaaa agagaatcat ttattgaaga agggaaaaat ttgataaata ctaaaaatgg
     2461 acaatataag aaagttatgt tggtgaataa tacaaattca caagttacac attcatttga
     2521 tgctaaggta attgagaatg taaaggttgg agatgttata gacataaaat tacctgtata
     2581 caaagatggt actcaaaagt atgaaacatt taaggttgaa gttagtgcga ttatgaaaga
     2641 agtatatgct gcagctcaag atggcaatgt tcatgctagt ggagcacaag taatatttag
     2701 agaagatgac tatagagaac taacaggtca aaaggaatat aataagcttt atgtgatggc
     2761 tcaaaaaggt aaattatatc cagtggaaaa aaaattagaa gaattaacaa agaattattc
     2821 atttacatca attggtggaa aaggtgagga tttgaaactg gttggagtac aacaaagctc
     2881 agaggaaagg cttgcagtaa tatatcaatg cctaattata cttatacttg ctgtaaatag
     2941 tattttcatt atgagaagta acattatatc aaggagaaaa gaattgtcaa cgctaagagc
     3001 aattggaatg agtatcaaga gtattaaaaa agttctaata attgaaagtg aattatatgg
     3061 aatcattgca actataatag gaactataat tgcaaccata tattataact ggggaatttc
     3121 aaaatcaaat aaaactcttt tagcaggagg atttaatagg actatagaat ataacattcc
     3181 ttttaatcag atatttatac tatttgcgat ttttattatt atggggtttg ttgcagtata
     3241 tctatctaaa gataagattg aaggaacaga gataacagag ggtatttcac aaaatgatta
     3301 agtttgagat tctaagataa catggggtga taagattgtt tgatgatata cagataaagt
     3361 ttagagaaaa tgatttatta ataaacaaaa gaaatggaaa agttattcta tataataaag
     3421 aaaaaaagag acatgtaaaa ttatcagaag aagtatatga atatactaga cttgctgaaa
     3481 aaaacaactg gaaagtcaga gaattaatag attgttttaa aaaagatgaa gataagaaat
     3541 atatcgaatc tatattaaat attatggtaa aaaacgaagt aattgatggg aatctattca
     3601 acaaaaaaaa ctttaaagac atgcatcttt ctcttacaaa tagatgtaat cttagttgta
     3661 atcactgcgt ttcaagttgt agtcctaaag aagaagatta tatggatact agtatgattt
     3721 taaaattaat agataattta tgtgagttaa atgtaagaag tttggtttta actggtggag
     3781 agccattggt cagagaagat ttttttgaaa ttgtgaatta tatcaaaaaa aaacttccat
     3841 tagaggaact tagtttatca acaaatgcaa cattaattaa tgatacaaat attgacttta
     3901 ttatcagtaa atttcataaa gttgatataa gtttagatgg aattgatgaa gaaacatgta
     3961 gcaacataag aggaaaaggt gtatttaaaa aagtattaaa tgtagtgtat gaacttcaaa
     4021 ataagaaatt taataatata agtttatcta tggtttttgg tgaaaaaaat tatcaaatgt
     4081 cagaaaaatt taaaaaactt aatgaagatc ttaatacaaa agctattgaa agatattttc
     4141 ttccgagtgg aagaggtcta ataaatagaa aaatttactc agatgaaaac tctgttttac
     4201 cacttgtgat acctaagttt cgtgaaaatg aaattaatgg taagaagaat aggataaatc
     4261 cgtgttcgtg ttcatgtaat gcttgtattg agcaagtatt tatagatcat aagggaaata
     4321 tatatccttg tccctctctt gtggaaaagg aatacttagt tggaagcgta tttgataaaa
     4381 aaattataaa taacctgaaa gagaatgagc taaaagaaaa tcaagctttt aggaatctta
     4441 atgaaatata tccatttaac tttgaaaaat gtaaagattg tgatgtgaat attttttgtt
     4501 ttaagtgtcc agctatggta aacaaagtaa aaaatgatga taaagaatta aacatatggt
     4561 gtaatttaac gaagagaaat atggagctag cagtttgggg aggtgaagta taaatgaaat
     4621 ttacgatatt tgttacagaa aaatgtaatt taagatgtac atattgctat gaacgaggaa
     4681 tggatagagg taagagcatg gatataaata ctgctaataa aaccatagac tttatttgta
     4741 agaatattaa aaatgaagaa cttgatattc cattatatat tgtatttcat ggtggagaac
     4801 ctcttataaa ctttaaatta gtaaagcata tacataaaga gctatccaaa agagtattag
     4861 aaagaaaaat tatttatgat atgacgacta atgcaactta tttggatgaa gaaattataa
     4921 attttatgtg taaaaatata gataatattt caattagtat tgatggaact aagaaatttc
     4981 atgataaaaa tagaatattt tcaaatggtg atggtagtta cgatttagtg ataaaaaatg
     5041 ctaaaagatt tttagatata ggcaaagatg tcagagtaag aatgacatat acaacagata
     5101 caatttctga actatatgaa agtatagctc aaattgtaag gtatggattt actaatatag
     5161 taccaattgc tgattttatg agtttaaact ggacaaataa tcatatagat ataattaatg
     5221 agcaaatttc aaagttaata aatttaaaaa aagaacatcc gaacatagat attagcttac
     5281 ttgatattaa aaatttgaat attaaaaagg gagactgttt tggtggaatt gtcagttttg
     5341 caatagatgt taatggagat atattcccat gtacttttag tgttggagat aatgagttta
     5401 aacttggaag tgtctttgac aggaatatta gtgaagaaaa aagacaagag cttattaaaa
     5461 tttatatttc agagaatgaa gagtgtaagg attgcttaag gaaagagtgg tgtacttcag
     5521 ttagatgtaa acttatgaat agaaaagtta caggggaatt taataaagca attccaataa
     5581 tatgtgcact ggagaaagtt atcttaacaa caacaagaaa aataaagatg gaagagaaat
     5641 ctaaagaggc aatgatatag cataaaaatt taatgtggaa gtacaattct ttatactgtc
     5701 ataaatatag ggggtaataa aatatggaag atagattttt tataaatgat gtatgtgaca
     5761 acataataaa tgaaaatcaa gtaattcttg ctaataagat aaatggtaaa tggataaaaa
     5821 ttcctatcga atgttataaa gttatagaat attcaataaa aaataaaaaa cctcttgata
     5881 aactggaaga tgtatttaat cttgatgaag ataaaaaata ttataaacaa gttgtagata
     5941 aattagaaca attaggtttt gtaagtaatt ttattgtaga taaatttaaa ttagagagag
     6001 tgaatttagt ttcattttct ccaacaaata gatgtaattt aagatgcgat tattgttgtg
     6061 cagattctaa aattgaaaat aaggattatt tgggtacaga ggagttaaaa gaagcaatag
     6121 ataatatagt taaaataaac ccaagaattt taactattac aggtggagaa ccattactta
     6181 gggatgattt ttttgaaata ctatcttata caaaagaaaa attcaaagga aagataaggt
     6241 tatctacaaa tgcgacttta atcaaagaga atcaggtaga tgagataatt gatggtttat
     6301 atgcaatgga aattagctta gatggttatg atgaattaag ctgtagtaag gtaagaggaa
     6361 aaggtgtatt tactaaagta ataaatttaa ttaaatatat taaatcaaaa ggatttaata
     6421 atattggttt gtctatggtt gtaggaaaaa acaatgaaaa tgacatagat aaatttaata
     6481 agataaatta tgaattaggg acaattccta taataagaaa ttttatgaat attggtagag
     6541 gaaatgaaag ttatactaga tatcttgatg atgaacttga tattggatat atttgtgaag
     6601 atgattatat aaataaaaat agtttaaatt caaatatgtg taaagctggt gtaaaccaaa
     6661 taagtattga ttataagggg gatgtatatc catgtccaaa cttagagtat gatgatttaa
     6721 agatgtttaa tatattacaa tttgatgaaa gtatgttaga aaaaatttta aacagaaata
     6781 tggaaatatt tgataagttt gatattttaa agcctatgaa catggaagaa tgcaaagatt
     6841 gtaaagtaaa tgtattttgt acaacatgcc catctaaaat gtatacttta aaaaataata
     6901 aagagatgtt aaattcaaat tgtaaaaaaa tgaaaagtat tttagagcct attgtgtggt
     6961 aaatactatt attttaaata tatacatact aaaaatattt ttataatata aagatttaag
     7021 aaaggtaaaa gttggttgtt ggatggaaaa aataaaagta gcagtagttg atactggcat
     7081 agatgtaaat gatgattcta taaaaaaata tataaaattt aataaaaata ttcagcttga
     7141 agaagtagtt gaatataaag atttagatga tacacatggg catggaactc tttgttcaaa
     7201 aacaattcta tcagtatgta gaaatactga taggattgaa atttatccaa taaaaatttt
     7261 taatcaagga gggtgtacta gtggagagag attattagaa gcattagaaa gaaatttaga
     7321 ttcagatatc gatttaataa atatttcagc atctacttta aatgataaat ataagcgaga
     7381 attaaaatat atatgtgaag aactttgtaa gaaaggaaaa attataatat catctcacca
     7441 taaaagagct aaggattgtg atagttttcc tactgttttt aattctgtta taggtgtaac
     7501 tggaagtaat gatatttata atgataatga ttatatctat gaaaagggaa aaaagattca
     7561 aattatagcg aatacaaaag agtgttttgt tgaatttagg aatgaagtaa ctcattttgg
     7621 aaagagcagt agagcttcag ctgttataac agggactatc tgtaacatat ttaataaata
     7681 tggaaaaata atgtttgatg aacttgaaaa aatttttgaa aaagaaagct tagataaaaa
     7741 tattaataca gttaaatcaa aatttagaaa taagcagaat aattttgatt tatgtattac
     7801 caaaaaccaa aaattagata tttcaagacg aattgtaaat atgataaata aaaagtttgc
     7861 tgtacataaa atagacttag attttttaga taaatattct atatttaata actttacaga
     7921 tttaggaaat cataatgcgt ttaagtttct tacagagata aataaagaat ttgaaattga
     7981 gataagttat aaaggattgt ttttatatga gctagagaat ttaaatgatt tggtgaattt
     8041 ggtatgtaaa catttgaata aagctagtgt caataactct atttaactaa aatctatttt
     8101 acataaacga aaatacaata aaattgaata ttttatttga tattttaaaa ttgcacatta
     8161 ctagaaaaga aatggaggaa ataatgaaaa cactaaaaat aaaaaattta aaaaagatat
     8221 atggtaaata tgaaaatgaa gttcatgcaa tagcaggaat tgaccttgaa atagaaccta
     8281 aaaagtttac agcaatagta ggggctagtg gttctgggaa aagtacatta cttcattgta
     8341 tggcaggtct tgacaaacca acatcaggaa acgtgtattt agatgattta gatatttata
     8401 cattaaatga tgataaatta tcaaaaatac gtagagaaga gtttggattt atatttcaaa
     8461 gttataatct tatacctgtt ataaatgtat atgacaatat agtattacct gtattattag
     8521 attcaaaacg agaggataaa acacatataa ataatcttat attccgttta ggattagagt
     8581 cacaagtaaa aaggtttcct aatgagcttt ctggaggtca acaacaaaga gttgcaattg
     8641 caagggcctt agcaaataaa ccatctgtga tatttgcaga tgaaccaaca ggaaacttag
     8701 atagtaagac tacagcggaa gtaatggagt tgcttgaaat ttgtgtaaag gagttttctc
     8761 aaacacttgt tatgattact catagtgata aaatagcaga gactgcagat aaaataatta
     8821 ctataagtga tgggaaaatt gtgaaaattt tagataaaag ttttgaagag cataatatgg
     8881 gtgaaactga aattaaaatt taatgttgtt aaagaagaat aacttttgaa taataaaatc
     8941 catatagtaa agacatatca ataatataga atattaaaat aaaaagtctt ggaagttata
     9001 tttagtattg attaataatt aaataaggtg gatgaaatga agaaaaagtg ttctaaaaat
     9061 aagaaaatag aaaataggaa taataagaat aagagcttaa aacgatttgc attccttact
     9121 tttaagaaga ataagggaaa gcttttactt tcgtttatta tggtaataat tgtagcagta
     9181 gttgatttag tacagcctca agtaacaaga atgatagttg atgaagggat aggaacaaaa
     9241 gatgctaacc ttcttttaaa actaatgttt atatatggag ctgctggagt atcttcagca
     9301 atactttatc ttcttttatc atatttatat tcggctataa aaaagaatgt aacagtagat
     9361 ataaagataa aattactcaa tcatttatct aaactatcag gaaaatacta ttcaaatata
     9421 aagacaggga atattatttc aataattgaa agtgatatat ttataataga aaattttgga
     9481 gcagaacttg cattttctat attagtaaat atatttacag cttcaatggc tttatttttc
     9541 ctagtaagaa tgcaagttga tttgtttttg atagttctaa tacttcaaat aattttaagt
     9601 atttttcaaa ataaatttac caaacttata tctaaaaaca caagagagat aagggaagat
     9661 tcaggaagtt tatcaaataa aatccaagaa tatgtttcaa atatcaagaa tgtagttgtt
     9721 tcaaaatcaa gaatgaagtt tttttcatca tatctaagtg aagaaaaaaa tcttataaga
     9781 aagtacttaa aactggattt aataatgaat gggaatattg caatcgcaaa atctattagt
     9841 atcttagtaa cagtatccat ttatggtgtt ggagggtaca agataataag tggaaatatg
     9901 acaataggag aactgcttgc atttcaacaa tatacaaata tgtttattgg accatgtata
     9961 agtatagtga gagcaaatac aagaatacaa caggctaaag catctttaga tagagtatac
    10021 tcagtaatag atgaggaaat agatataaaa gtaaataata gaggattgag aatagaggat
    10081 aaagagtgca atataataga atttaagaat atctattttt catataaaga aataggaaag
    10141 aaaaaattac aaaatttaga acaagatgag caaattgatt gtacatatga agatatagaa
    10201 acagatagtt atattctaag taacataaat attacattta gaaagggaga aactacagct
    10261 cttattggtg aaagtggaag tgggaaatct acaatagtaa atttacttta cagattatgg
    10321 gatattcaaa aaggtgaaat aactattgat ggaataaata taaaaaatat aaatctaaaa
    10381 tctcttagaa aaagtataaa tattgttact caagatatgc ttatgtttga cgatacaatt
    10441 agaaataata taaatataaa taaaagttta agtgatgaag aacttaaaaa tatatgtagt
    10501 gtagtaggaa tgaacgattt tataagtaga cttgaaaatg gatttgatac aattatagga
    10561 gaaaaaggag tcaagatatc aggtggtcaa aaacagagaa ttgcactcgc aaggtcatta
    10621 gtaaatgata gtaagatatt gatactagat gaagcaacat ctgcacttga caatatatcc
    10681 caaagcgaga tattaagaaa tattagggcg tatgtaaaag gtaagatagt gattattata
    10741 gcacatagac tttcaactat aaaagatgca gacaatattt atgttcttga aaaaggaaaa
    10801 gttgtggaat caggtaaaga taaagaacta tttataagaa atggtatata tagaaaacta
    10861 gttactttaa atacacaata gaattattac ttatagtaga aatagatata attcaataac
    10921 tttaatggaa tatacagaat attatttaaa atattgttat gaaaaaaagt aatcatttta
    10981 tttattttat ctttgttaaa gtattttaaa ttttcgtttg agacaagtaa gaatttttct
    11041 aaggtttcac ggtatagcat tttctttatg ttataccgtt ttttcttaaa gaagaaagag
    11101 tagacaatct attgaaatca caacaaggca tacctatcca aagcgtaata agttatgtcc
    11161 acaccattca tctgctaaaa ctaagcattc aaaaaactga tgacaaatcc tataaacact
    11221 tcctattctc acttcaaaat taaaatttat cagataattt cttgtataaa ttatactatg
    11281 gctttatatt ctagttgctt ttttttattt gataaatata cttattgttt aataatctct
    11341 ctagaagaaa ataatgaaga aacaaaaata tcacgtgacg acagtttagc cacaaattac
    11401 tttctagatt gtctaaatta ataggggctg aagttatgag taaaaaaata aacgtataac
    11461 atagactata aatgtgcgac ataaagcatg gatatatgat ataaactttg aatagtatat
    11521 tataatatag gtcaaagtat caaatgtaga aggattatat ttattacaaa ctaatattga
    11581 caaataaaag gcttgctctt atatggatat gtccctgtca agtagacaga cttaaaaaag
    11641 gctatcttta cgcacaatga gttcgatatt caatcggact cattccattt tttaattgta
    11701 ttctttcatt attataaaaa taaatataat catttattag actaatcatt ttctttggat
    11761 ctgtagtatc aattaaatat attaattcag atttaaaatg actgaaaaaa ctttctatag
    11821 gagcattatc ccaacagttg ccccttcggg acat
//
LOCUS       DAEPXF010000116        11836 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain HB27
            SAMN13376389-rid12479423.denovo.122, whole genome shotgun sequence.
ACCESSION   DAEPXF010000116 DAEPXF010000000
VERSION     DAEPXF010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13376389
            Sequence Read Archive: SRR10518432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 11836)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11836)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001069624.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001069624.1
            Genome Coverage       :: 315x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/15/2021 01:54:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,165
            CDSs (total)                      :: 4,076
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 89
            rRNAs                             :: 10, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 10 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 19 of 69
            Pseudo Genes (incomplete)         :: 44 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 10 of 69
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11836
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13376389-rid12479423.denovo.122"
                     /strain="HB27"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="China: Hebei"
                     /collection_date="2017"
     gene            70..1074
                     /locus_tag="KRL67_002659"
     CDS             70..1074
                     /locus_tag="KRL67_002659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HBH1523525.1"
                     /translation="MRFKRILILMLTVVMIVGMFVGCDNNPSNNPSSSKDNKSDVSDK
                     SNSKKSVSMILDIEGTNNEAMNNSALLALNNAQKKLNIDTNKVESDDSSTFSNSIDIL
                     CNDNYDLIIAVGARFAKPLEMVAKKYPKQQFAIIDYEYDKQPSNITSISYEDNKSGYL
                     AGLIAGKMTESDKVGFIGGTKSSSRDKFESGFREGVKFSNSSIKDISVEYADVFKGSK
                     SVESIAKKMMDNGVDIIFSTTEDDSKAVIDAVRSKNKKVIATNKDQHELAPENVISSI
                     VKDFENPTYNLIQSFVKGNYKGGKVIENTVKSGSTGLAQNSSQNIPPDVLEYVNKNNK
                     "
     gene            1200..2312
                     /locus_tag="KRL67_002660"
     CDS             1200..2312
                     /locus_tag="KRL67_002660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HBH1523526.1"
                     /translation="MKIKRILMACFVVVFTIFILAICNFDFGFDKVNKNNSNIDIAKS
                     KQSESKSVPTSSEKDKSNNKSKNKEDDTEDDSYVRKTIGVLLDTENLNNESYNNNVLS
                     ALKRADEDFDVRIKLEKPKTSKSFKESIEKLHKYEPDLIIAIGARFAEPIKEAATKYS
                     KQQFAIVDYTYKVQPANVISVSFQDNVTGYLAGLIAGKMTDTGKVGFIGGVEGSSRDK
                     YESGFRKGLKTSNKDAELLVKYSNIFENPESARTIAKEMRDSDRDVVFSATEDDSKRA
                     IQVARENGGKVIAINKDQYDMAPDNVISSIIKDVEQPVYELIRSVVKDGFKGSKVMEF
                     KIKDGELGLTSKSSRNIPPDVLEYIKKEYNKVKDTY"
     gene            2601..3362
                     /locus_tag="KRL67_002661"
     CDS             2601..3362
                     /locus_tag="KRL67_002661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH1523527.1"
                     /translation="MEFTREPFILDDDLCLALVDKRISYLMENELKLRNIEIIKTVEC
                     KELYESIKYHPDICICNLGKGDIIVAPNVYNQYKELLSRYKFNIIKGEANLLNRYPYD
                     IPYNIAIVGDYAIHNFKYTDKAILDYIENNKLKKINIEQGYSKCSICIVDRTSIITSD
                     KGIWKSMKNTEIECLLIEKGHINLFEMNYGFIGGCTGLISKDKLAFCGDVKKHPDYER
                     IKSFVESKNKEIVTLSCENLLDIGSIVPLMTRKER"
     gene            3365..4183
                     /locus_tag="KRL67_002662"
     CDS             3365..4183
                     /locus_tag="KRL67_002662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative sporulation protein YtxC"
                     /protein_id="HBH1523528.1"
                     /translation="MVEKDNISSMKFLRNNNVNDRLIYTNDIVEVVIDSAKQEVLNKK
                     ILNGTKAEQRALNIDEISTEITSMIISIMKEKLLKEYILKCYGRTYPEDKNNIYIYSL
                     NIFAKKEIFMRETVFTRVRNYIEENDFINVEGFIRFRMREFMKYISAIGDIAVEEYLI
                     KKDQDEFIRVLKYFIDTQEEKIDLLKVHIMEDNTFVLYDKNGNKIDSIDDEEIINMVI
                     RENLNYEDFLISTLLTLCPKKIEILDLLNNNCSKEIIDTVEAIFENKVSIIMEN"
     gene            4363..4965
                     /locus_tag="KRL67_002663"
     CDS             4363..4965
                     /locus_tag="KRL67_002663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF445 family protein"
                     /protein_id="HBH1523529.1"
                     /translation="MDNLIRILILAVIGGFIGYVTNVVAIRLIFRPIEPIKIPILNIE
                     IVGLIPKRRAEIAANVGEIIQEEFLSMDEILANIITDEDKEEVVRYIKARVKIIIHEK
                     VSFIPSGIKNMIQDYLGEIIESEVKQSIDELSKNIINKANERIDIQKMVEDKINELDL
                     YELEEIIIRIAKKELKHIEFLGLVLGFLIGIVQGLVTMFI"
     misc_binding    5341..5573
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            5637..7556
                     /gene="thrS"
                     /locus_tag="KRL67_002664"
     CDS             5637..7556
                     /gene="thrS"
                     /locus_tag="KRL67_002664"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HBH1523530.1"
                     /translation="MIKVALKDGSIKEFENAISVMDVAKSISEGLARNVVAASVNGEV
                     VGLDHIIDTDCDLNLFKFEDKEGKEVFRHTSAHILAQAIKRLYPEAKLAIGPSIENGF
                     YYDIDLDHRLVPEDLEKIEAEMKKIAKEDLKIERFELPRNEALELMKEQGEDYKVELI
                     SDLPESEIISFYKQGDFTDLCRGPHLPSTKKVKAVKLQSVAGAYWRGDENNKMLQRIY
                     GTSFEKNKDLEEYLHLLEEAKKRDHRKLGKELGLFMIPEEGPGFPMFLPKGMELKNEL
                     LKFWREIHRKAGYIEIESPIILNRKLWETSGHWYHYKENMYTVKIDDEDYAIKPMNCP
                     GGLIYYNSQLHSYRDFPMRVAELGRVHRHELSGALQGLMRVRAFTQDDSHIFMLPEQI
                     KDEIKGVANLIDGIYKTFGFEYNLELSTRPENSMGSDEEWEAAENGLREALEELGLPY
                     TINEGDGAFYGPKIDFHLKDCLGRTWQCGTIQLDMQLPRQFDNTYIGQDGEKHRPVMI
                     HRVAFGSIERFIGILIEHYAGKFPVWLSPTQVKILPISDKFMDYANEVKKELFDKGIR
                     VELDDRAEKIGFKIREAQLEKVPYMLIVGEKEVADNNVSVRSRDKGEIGSIKLDEFIA
                     SISKEIESRESIIQD"
     gene            8322..9437
                     /locus_tag="KRL67_002665"
     CDS             8322..9437
                     /locus_tag="KRL67_002665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HBH1523531.1"
                     /translation="MIEKVEEKSKIIKTSKEYKRTIVEEKDKEWTKKLNDILSRSSFS
                     NDELDKIKELIPKEILTSENVGEVVTEDGYAKPEYDEVFKSLFTLNNEYDLLVNFIND
                     ILKDAPYANRNIKPFTQIKRIVRVETDPTINYIGEKRPRLDILAEDEENNHINIEMQR
                     AFENDYLERAEYYLSRVHGRKLEEGKEYKEIGKTIGIHILNHVKYNHIEDYVNCLRLT
                     MDGHPDIYSSKTALYFIELPKIHKSDYIVNRIMLWGKLISNPSSLDVRVIAKNDSIIK
                     KALDRLKELGSNEEYLLNLKRGAYIMNKSRNFKEEIERETETRVARETAIRVAKEKDY
                     KIIIMLKKNGFKLDDILNKFNDFDLSEDEIKEVYENN"
     gene            complement(9659..10696)
                     /locus_tag="KRL67_002666"
     CDS             complement(9659..10696)
                     /locus_tag="KRL67_002666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat protein"
                     /protein_id="HBH1523532.1"
                     /translation="MNKKLIKAKIFTWSIVAISLSIVFVSLLIGTIISHSFNFPFNIL
                     NPKYNYDNYIVAKNESFSDKINDINVDWISGEVIVKRSDSNKIKLIQKIPSNFSKKKL
                     AKIMVDGDSLSIYDNSAKKFFIGINLPKPTILELYLPNKLYDKITLYTTSADIYSTYI
                     RSNTVEANTVSGAIGLSGNINKINLDTTSGHIDIAKLNSKNATLKSTSGEIQLSGKID
                     VLNLETTNGNINLKDIYNKNLTCSSVSGKMNLSGVFSDIKAESTSSNIKITSSKMLSS
                     LYCETTSANVDLSIPDNPGFTLNFDKVSGVLDSDFELNRHSDLYTYKNGITDLYLSTT
                     NGNLTIIKNNH"
     gene            complement(10689..11375)
                     /locus_tag="KRL67_002667"
     CDS             complement(10689..11375)
                     /locus_tag="KRL67_002667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH1523533.1"
                     /translation="MLDKIRTYIDNSFTGVNETKKVKELKDELFENLKEKYNDQIQDG
                     KTEQEAYNSVISGIGDLSELIESVKEPYSLPSELIEEKKKRALRASIAVALFIISPFL
                     FVVSVVSFGIEPTIAILLMLILIALGVGLLVYNQMTRTNYALYNDNLSNKFKEFEDFE
                     ENYNHINPVYKSFINAYWIIIVAIYLITSILFNIWFFSWILFIIGAAGINIFKGIIQL
                     KGYKEDDKYE"
     gene            complement(11387..11707)
                     /locus_tag="KRL67_002668"
     CDS             complement(11387..11707)
                     /locus_tag="KRL67_002668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBH1523534.1"
                     /translation="MTIASDMIRGHTETIILKHLLEKDSYGYEINKSILEKTKGLYEL
                     KEATLYTAFRRLEQSGAITSYWGDEVTGARRKYYSITSIGKEKYIKSREEWNNTKALI
                     DILI"
BASE COUNT         4660 a         1264 c         2035 g         3877 t
ORIGIN      
        1 taaaagtaaa taggatgtaa aagtaaatag gatataaaaa tatcgtaaat gtatgaggag
       61 tgaaattcaa tgcgtttcaa aagaatattg atattgatgt taactgtagt aatgatagtt
      121 ggaatgtttg ttgggtgtga taataaccca agtaataatc ctagctctag caaagataat
      181 aaaagtgatg taagcgataa aagtaattca aaaaaatctg tatcaatgat actggatatt
      241 gaaggaacaa ataatgaagc tatgaataat tcagctttat tagcattaaa caatgctcaa
      301 aagaaactta atatagatac aaacaaagtg gaatcagatg attcctcaac tttttctaat
      361 agtatagata ttttatgtaa tgacaattac gatttaatta tagctgtagg agctagattt
      421 gcaaaaccat tggagatggt agccaagaaa tatccaaagc aacaatttgc aattatagac
      481 tatgagtatg acaagcaacc ttctaatata acttcaattt cttatgaaga taataaatct
      541 ggatacctag ctggattaat tgctggaaag atgactgaaa gcgataaggt tggttttata
      601 ggaggaacaa agagttctag tagggataaa tttgaatcag gttttagaga gggtgttaaa
      661 ttttctaata gtagtataaa ggatatttca gttgagtatg cagatgtttt taaaggtagc
      721 aaatcagtag aatctatagc taaaaagatg atggataatg gagtggatat tattttttct
      781 actactgaag atgattcaaa agctgtaata gatgcagtaa ggtcaaaaaa taaaaaagtt
      841 atagcaacga ataaagacca acatgagtta gctccagaaa atgttataag ttctattgta
      901 aaagattttg agaatcctac ttataatctg atacaaagct ttgtaaaagg aaattataaa
      961 ggcggaaaag ttatagaaaa cacagttaag tctggaagca ctgggcttgc ccaaaatagt
     1021 tctcaaaata ttcctccaga tgtgttagaa tatgttaata agaacaataa ataatctact
     1081 ataatatcca agaatactta tgactttagt tatgaggtga attggatgtt ataatagaca
     1141 tatatagaaa gacatatgca gaggtaggtc agacctatct aaatcaggga gtgaattttg
     1201 tgaaaataaa aaggatattg atggcttgct ttgtagtagt ttttacaatt tttatattag
     1261 ctatatgtaa ttttgatttt gggtttgata aagtaaataa gaataattct aatatagata
     1321 ttgctaaatc gaaacagagt gagtctaaaa gtgtaccaac ttcatcagaa aaggataaat
     1381 caaataataa aagtaagaat aaagaagatg acacagaaga tgattcatat gtaagaaaaa
     1441 ctattggagt attgttagat accgaaaact taaataacga atcatataat aacaatgtac
     1501 tatcagcctt aaaaagggct gacgaagatt ttgatgttag gataaaactt gaaaagccaa
     1561 agacttctaa aagttttaaa gagagtatag aaaaactaca taagtatgag ccagatttga
     1621 taatagcaat tggagctaga tttgcagagc caattaaaga agctgctact aaatattcca
     1681 aacagcaatt tgcaatagtt gactatactt ataaagttca gccagctaat gtaatttctg
     1741 tatcatttca agataatgtt actggatatt tagcaggact tattgcaggt aaaatgactg
     1801 atactgggaa agtcggtttt ataggaggag ttgaaggttc tagtagagat aagtatgaat
     1861 ctggatttag aaaggggctt aaaacttcaa ataaagatgc tgagttacta gttaagtatt
     1921 caaatatatt tgaaaaccct gaatcagcta gaactattgc aaaggaaatg agagattctg
     1981 atagagatgt tgtattttca gctacagaag atgatagtaa aagagctatt caggtagcaa
     2041 gagaaaatgg tgggaaagtt atagctatta ataaagacca atacgatatg gctccagata
     2101 atgtaataag ttcaattata aaagatgtag aacaacctgt ctatgaactg ataaggagtg
     2161 ttgtaaaaga tggttttaag ggaagtaaag taatggaatt taaaataaaa gatggagaac
     2221 ttggcttgac ttcaaagagt agtagaaata taccaccaga tgttttggaa tacataaaaa
     2281 aagagtataa caaggtgaag gatacatatt aattattgat gtagaagatt ataaaaatat
     2341 actgattaaa tttagattag caataaaaag atatatttat taaatttgtg ctaactgtac
     2401 aaaatatatt gattaagttt agattagtta taaaaagata catttattaa atttgaacca
     2461 actgtacaaa actatactaa ttaagcttag attaactata taaaattata acaataagtg
     2521 tgtgggcaga aaaattctgc tacacacttt atttatattc acataaaaaa tttatcaaaa
     2581 aaaagaaaaa ggagtaaaaa atggaattta caagagaacc atttatacta gatgacgatt
     2641 tatgtctagc tttggtggac aaaagaatta gttatctaat ggaaaatgaa ttaaaattaa
     2701 gaaatataga gataataaaa acagtagaat gtaaagaatt atatgaaagc ataaaatatc
     2761 acccagatat atgtatttgt aatttaggaa aaggagatat aattgttgcg ccgaatgtat
     2821 ataatcaata caaagagtta ttaagtagat ataaatttaa tataattaag ggagaagcta
     2881 atttattaaa tagataccct tatgatatac cttacaatat tgcgatagtt ggtgactatg
     2941 ctatacacaa ttttaagtat actgacaaag caatattaga ttatattgaa aataataaac
     3001 ttaaaaagat aaatatagag cagggctatt ctaaatgctc aatctgtata gtagatagaa
     3061 cgtctataat aacatcagat aaaggtattt ggaaaagtat gaagaataca gagatagagt
     3121 gtttattgat agaaaaaggt cacataaatt tatttgagat gaattatgga tttataggtg
     3181 gatgtacagg gttgatttct aaggataagc tagctttttg tggagatgtt aaaaaacatc
     3241 ctgactatga acgtataaaa agttttgtag aaagtaaaaa caaggagata gttacactta
     3301 gctgtgaaaa tttactagat ataggttcta tagttcctct catgacaaga aaggagcgat
     3361 aactttggtt gagaaggata atatttcttc catgaaattt ttgaggaata ataatgtaaa
     3421 cgatagatta atatatacga atgacattgt agaagtagtg atagattctg caaagcaaga
     3481 agtattaaat aaaaaaatac ttaatggtac taaagctgaa caaagagctt taaatattga
     3541 tgaaatatca acggaaataa ctagtatgat aataagtata atgaaagaga aacttttgaa
     3601 agaatacatt ctaaaatgct atgggcgtac atatcctgaa gataaaaata atatatacat
     3661 atattcactg aacatatttg ctaaaaaaga gatttttatg agagaaacag tatttactag
     3721 ggtgagaaat tatatagaag aaaatgattt tataaatgta gaaggtttta ttagatttag
     3781 aatgagagaa tttatgaaat atatttctgc tataggtgat attgcagttg aggagtattt
     3841 aataaagaag gaccaagatg aatttataag agttttaaaa tactttatag atactcaaga
     3901 agaaaagatt gatttgctta aagttcatat aatggaagat aatacatttg ttttatatga
     3961 taaaaatgga aataagatag acagtataga tgatgaagaa attataaata tggtaataag
     4021 agaaaattta aattatgagg actttttaat aagtacctta ttaactctat gtcccaaaaa
     4081 aatagaaata ttggatttgt taaataataa ttgctcaaag gaaattatag atactgtaga
     4141 agcaattttt gaaaataaag ttagtataat tatggaaaac tagaatctaa gtatataaaa
     4201 gttagccata taaaactaaa aaaattcttt ttaaatcgac acaaaagact tataattgta
     4261 tataaagcta tagtataact agagttatta agtataaaga atatctactt gattaatgca
     4321 atatagctgg taaatataca attgataagg agtaatttta taatggataa tttaattagg
     4381 atactgatat tagctgttat tggtggattt ataggttatg ttacgaatgt agttgctata
     4441 agactgatat ttagaccaat agagcctatt aaaataccta ttttaaatat agaaatagta
     4501 ggtcttatac caaagagaag agcagaaata gcagcaaatg taggagaaat aattcaagaa
     4561 gaatttcttt ctatggacga aatacttgca aatatcatta ctgacgaaga taaggaagaa
     4621 gttgtaagat acataaaagc aagggtaaaa attatcatac atgagaaagt ttcatttatc
     4681 ccgagtggta taaaaaacat gatacaagat tatctaggag agataattga gtctgaagtt
     4741 aagcaaagta tagatgaatt gagtaaaaat ataataaata aagcaaatga acgtatagat
     4801 atacaaaaaa tggtcgaaga taagattaat gaactagatt tatatgaatt ggaagagata
     4861 ataataagaa tagctaagaa agaattaaaa catatagagt ttttggggct tgtcttaggg
     4921 tttttaatag gtatagttca agggttagtt acgatgttta tttaaatata tgagaagtag
     4981 catttattta agtattaatt gtatttactt atttgactgt attacttatt aacagcattt
     5041 agatattaat tgtatttaag tatacaaaac ctaaaaagag tacaaaaatt agaagttagt
     5101 tttaataaaa attagtttta gtatttatct agtcatatct atcatgcatg tggatacgtt
     5161 ttaaaaattt taaatttatg aaaatattat aatatcacta taaatcaaga gattaattta
     5221 atataaaaat ataagaatgt tggtttacag ctgattgaga tgaaaaataa tgggtaatta
     5281 atatattaca ttaatgttga ttgatgtata actatctgtt ataatgaaaa tatattaaaa
     5341 atcatagtgt tgaagaggaa gagtagagta gaaaccattc tcagagaatt tgggtctatg
     5401 gctgagagcc catgtaatag gtctatttga aaactacctt ggagctgagc ttagtagcag
     5461 tatttatact atacgtgaaa gtgctcgggt gacgacctta tagtcatcaa gagggttgta
     5521 tatacttgta tacgccaact tgggtggaac cgcgaattaa tatcgtccca aatatatttg
     5581 gggcgatttt ttattacaaa aaatctaaaa attatttcaa taaggaggaa aaaacaatga
     5641 ttaaagttgc tttaaaagat ggctcaataa aagaatttga aaatgctatt tctgttatgg
     5701 atgtagctaa atccattagt gaaggattag ctagaaatgt ggtagcagcg tctgtgaacg
     5761 gagaggttgt aggtctggac cacattatag atactgactg tgacttgaat ttatttaagt
     5821 ttgaagataa agaaggtaag gaagttttta gacatacatc agcacatata ttagctcaag
     5881 ctataaaaag actttaccca gaagcaaaac ttgctatagg accaagtata gaaaatggat
     5941 tctactatga tatagattta gaccatagat tagttccaga ggacctagag aaaatagaag
     6001 ctgaaatgaa aaaaatagct aaagaagatt taaaaataga aagatttgaa cttcctagaa
     6061 atgaagcttt agagcttatg aaagaacaag gagaagacta taaggtagaa cttatatctg
     6121 atttacctga aagtgaaata atatctttct acaagcaagg tgattttact gatttatgta
     6181 gaggaccaca tttaccatct actaaaaagg taaaagctgt taaattacaa agtgtagcag
     6241 gtgcatattg gcgtggtgac gaaaataata agatgcttca aagaatatat ggaacttctt
     6301 ttgagaaaaa caaagactta gaagagtact tacatttact agaagaagct aaaaagagag
     6361 accacagaaa attaggtaaa gaattaggat tatttatgat acctgaagaa ggtccaggat
     6421 tccctatgtt tttaccaaaa ggtatggaac ttaaaaatga attattaaaa ttctggagag
     6481 aaatacacag aaaagctgga tatattgaga tagaatctcc aatcatatta aatagaaaat
     6541 tatgggagac ttcaggacac tggtatcatt acaaagaaaa tatgtataca gttaaaatag
     6601 atgatgagga ttatgctata aaacctatga actgtcctgg tggattaatt tactataact
     6661 ctcaactaca ttcatataga gatttcccaa tgagggttgc agagcttggt agagttcata
     6721 gacatgagtt atctggtgca ttacaaggat taatgagagt tagagcattt actcaagacg
     6781 attcacatat attcatgtta ccagagcaaa ttaaggacga aataaaagga gtagctaact
     6841 taatagatgg tatctacaaa acttttggat ttgaatataa tttagaatta tctacaagac
     6901 cagagaactc tatgggaagc gacgaagagt gggaagcagc agaaaatgga ttaagagaag
     6961 ctttagaaga gttaggtcta ccatatacta ttaatgaggg tgatggagca ttctatggtc
     7021 ctaagataga cttccatcta aaagattgtt taggaagaac ttggcaatgt ggtactatcc
     7081 aattagatat gcaacttcca agacaatttg ataatactta tattggtcaa gatggtgaaa
     7141 agcatagacc agttatgatt catagagttg cttttggtag tatagaaaga tttataggta
     7201 tattaataga gcattatgct ggtaaattcc cagtttggtt atctccaact caagttaaga
     7261 tacttcctat atctgataag tttatggatt atgcaaatga agttaagaaa gagttatttg
     7321 ataagggtat aagagttgaa ttagatgata gagctgaaaa aattggattt aagataagag
     7381 aagctcagct tgaaaaagtg ccatacatgt taatagtagg tgaaaaagaa gtagctgata
     7441 ataatgtttc tgttagatca agagataaag gtgaaatagg tagtattaaa ttagatgaat
     7501 ttatagctag tatttcaaaa gaaattgaat caagagaaag tattattcaa gactaatata
     7561 caatatatta gtagctaaga tattgttata taagataagt ttaatcaata tgttgatatg
     7621 ttgatgcata gtgattaaat ttatgaaaag atgagtataa ttagaggtac attaatcata
     7681 tttttatgat taatgtacct ctagtttttt gtatggtaga tttattaata aatatatcat
     7741 agaagtgttt tgaacaagga aatatttatg tgcattcttt aaataaaagt ttattttttg
     7801 agtgataatt aagtacttgt tggtaaggca tggctgtaaa aatcataact ttattttcat
     7861 atttgttcca ttttcataaa aattattatc cctttaagaa aatttcttaa tccccttgaa
     7921 atatttcctt tttagtctaa agttaaattt ctttcttcaa aatgaatatt taaatgaact
     7981 aaaatttaag tacattttct aatcttaatg agtctatttt tcatagataa ttgccctata
     8041 atagaaggag ccatgttggg gctatgtggt aacaataacg gcattgccag tgattaatta
     8101 atgttttgta aatattaatt aatgtcttat gttatttttg gacttacgcc ctatacatag
     8161 attaatttat ttaactttag atatagagag aatgtcaaat acatacacag acgctagagt
     8221 ttaagaaagt gtttatggag caagaacttt taaaagaggt agtaacttag cttaatttca
     8281 agaaatgata aatacaaaag agtaaaaaga gaggtgttga aatgatagaa aaagtagaag
     8341 aaaaatctaa gattattaaa acatcaaaag aatataagcg aactatagtt gaagaaaaag
     8401 ataaagaatg gaccaaaaaa ttaaatgaca tacttagtag aagtagtttt tctaatgatg
     8461 aattagataa aatcaaggag ttaattccaa aagagatttt gactagtgaa aatgttggag
     8521 aagttgtgac tgaagatgga tatgctaagc ccgagtatga tgaggtattt aaatcattgt
     8581 tcactttaaa taatgaatat gatttacttg taaattttat aaatgatatt ctaaaagatg
     8641 ctccatatgc taacaggaat atcaaaccgt tcactcaaat aaagagaata gttagagttg
     8701 aaactgaccc aacaattaat tacattggag agaaaagacc tagactcgat attttggctg
     8761 aagatgaaga aaataatcat ataaatattg agatgcaaag agcttttgag aacgattatt
     8821 tggaaagagc tgaatattat cttagccgtg tacatggtag aaagttagaa gaaggtaaag
     8881 agtataaaga aattggaaaa actattggaa tacatatact aaatcatgta aaatataatc
     8941 acatagaaga ttatgtaaat tgcctgagat taacaatgga cgggcatcca gatatttatt
     9001 ctagtaaaac tgcattatat tttatagagc ttccaaaaat tcataaatct gactatatag
     9061 taaatagaat tatgctatgg ggaaaactta ttagtaatcc ttctagctta gatgtaagag
     9121 ttatagctaa gaatgattct attattaaaa aagcacttga tagattaaaa gaattaggta
     9181 gcaatgaaga atatttatta aatctgaaaa gaggtgctta tatcatgaat aaaagtagaa
     9241 attttaaaga agagattgaa agagaaacgg aaacaagagt tgcaagagag acagcaatta
     9301 gagttgcaaa agaaaaagat tataaaataa ttataatgtt gaaaaaaaac ggttttaaat
     9361 tagatgatat attaaataaa tttaatgatt ttgacttaag tgaagatgag ataaaagaag
     9421 tctatgaaaa taattgatgt gtaaataaaa agtgaactta tataagtttg ttgaatctga
     9481 gtctggaaat atttatgtgt attttttaaa tgaatgaaaa tttattttaa agaccataat
     9541 ttagaggtac agttaattat atttttatga tactgtactt ctagtttttt ctattataga
     9601 tttattattt attaataaat ctacaataga agtgtttttg ttgtaagaat cattttatct
     9661 aatgattgtt ttttataata gttaaatttc catttgtagt gcttaaatat aaatctgtaa
     9721 taccattttt ataagtgtat aaatcactat ggcgattcaa ctcaaagtca ctgtccaaaa
     9781 caccagaaac tttatcaaaa tttaaagtga aacctggatt atcaggaata gacaaatcga
     9841 cattggcaga agtagtttca cagtacagag aagacaacat tttgctagaa gtgattttta
     9901 tattactgct agttgactca gctttaatat cagaaaaaac tccagataag ttcatttttc
     9961 cagaaactga actacaagta agatttttat tgtatatatc ttttaaattt atattaccat
    10021 tagttgtttc taagtttaaa acatcaattt tcccagaaag ctgtatttca ccagatgttg
    10081 atttaagagt agcattttta ctatttagtt ttgctatatc aatgtgacca ctagttgtgt
    10141 ctaaatttat tttattaata ttaccagaga gacctattgc acctgaaact gtgttagctt
    10201 caactgtatt acttcttatg taagtgctat atatatctgc tgaagtagta tataaggtta
    10261 ttttatcata aagcttattg ggcaaatata gttcaagtat tgttggtttt ggaagattaa
    10321 ttccaatgaa gaatttttta gctgaattat catatataga aagagaatct ccatcaacca
    10381 ttatttttgc tagttttttc tttgagaaat tactagggat tttttgaatt aatttgattt
    10441 tattagaatc acttctctta actataactt cacctgaaat ccaatcaaca ttaatgtcat
    10501 ttattttatc tgaaaaactt tcatttttag ccactatata gttatcataa ttgtatttag
    10561 gattaagaat attaaaaggg aaattaaaag aatgtgaaat tatagttcct ataaggagtg
    10621 agacaaatac aatagaaagt gatattgcca cgatactcca ggtgaaaatc tttgccttta
    10681 taagcttttt attcatattt atcatcctcc ttgtagcctt ttagttgtat aattcctttg
    10741 aaaatgttga taccagcggc acctattata aatagtatcc acgaaaagaa ccaaatatta
    10801 aatagtatgc ttgtgattag atatatagca actattataa tccaatatgc atttataaat
    10861 gatttataaa ctggatttat atgattataa ttttcctcaa aatcctcaaa ttctttgaat
    10921 ttatttgata aattgtcatt gtatagagca tagtttgtcc ttgtcatttg attatagact
    10981 aataaaccta ctccaagtgc tattaatatg agcattaaaa gaatagctat agttggttct
    11041 atgccaaaac ttacaactga gactacaaat aggaaagggc ttattataaa aagagcaact
    11101 gctattgatg ctcttaaggc tcttttcttt ttttcttcaa ttaattcaga aggtagagaa
    11161 tatggttcct taacactttc aattaactca gacaaatcac caattcctga gattactgag
    11221 ttatatgctt cttgttctgt tttaccatct tgtatttggt cgttatactt ttcttttaga
    11281 ttttcaaata attcatcttt aagttctttg actttttttg tctcattaac tcctgtaaag
    11341 ctattatcaa tataagtacg tattttgtct aacatatttt cacctcctaa attaatatat
    11401 ctatcaatgc ttttgtatta ttccattctt cacggctttt tatatatttc tctttaccta
    11461 tgcttgttat tgaataatac tttcttctag ctccagtaac ttcatcgccc caataagagg
    11521 ttattgcacc agattgttca agcctacgaa atgctgtata caaagtagct tctttcaact
    11581 catacaatcc tttagtcttt tcaagtatag atttattaat ctcataacca taactgtctt
    11641 tttcaagtaa atgttttaag ataattgttt ctgtatgacc tcttatcatg tcagatgcga
    11701 ttgtcatgaa catcacctcc gattaaatat ataatacttt gatatacttc gcctgtcaag
    11761 gtatatcatg agaaaagaca ttttattgta atatttataa gattttatat agaaataatg
    11821 taaaaacttt ataaat
//
LOCUS       DAEPXF010000117        11801 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain HB27
            SAMN13376389-rid12479423.denovo.123, whole genome shotgun sequence.
ACCESSION   DAEPXF010000117 DAEPXF010000000
VERSION     DAEPXF010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13376389
            Sequence Read Archive: SRR10518432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 11801)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11801)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001069624.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001069624.1
            Genome Coverage       :: 315x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/15/2021 01:54:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,165
            CDSs (total)                      :: 4,076
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 89
            rRNAs                             :: 10, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 10 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 19 of 69
            Pseudo Genes (incomplete)         :: 44 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 10 of 69
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11801
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13376389-rid12479423.denovo.123"
                     /strain="HB27"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="China: Hebei"
                     /collection_date="2017"
     gene            185..1225
                     /locus_tag="KRL67_002669"
     CDS             185..1225
                     /locus_tag="KRL67_002669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBH1523535.1"
                     /translation="MDKHSDSVMYGGMEVYNIWGTTTVDSIIGESDVMRALKNRICKI
                     GNSDSTVAITGESGTGKELIARAIHLAGNRGNKTFVAINCAAIPEPLLESELFGYTKG
                     AFSGADSKGKIGKFELANGGVVFLDEIGDMPIQLQSKLLRVLQEKKFARIGSNELIDI
                     DVRIISATNRDLFELVKENKFREDLYYRLNVIPLEVPPLRERGDDIQILINNFIDKYS
                     RKMKKNVYHIEPDVEQMLLKYPWPGNIRELENTIELALNLLEEDGIIHKGIINRKILE
                     YFKLNNINIKLLEESDYEISMEQDILTLEEFERAYIKKVLKFYGNDTKSKKLAAKKLG
                     ISLATLYRKIDV"
     gene            1581..2903
                     /locus_tag="KRL67_002670"
     CDS             1581..2903
                     /locus_tag="KRL67_002670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine permease"
                     /protein_id="HBH1523536.1"
                     /translation="MFDIDGRPPILKCLPLSMQHILAMIVGTVTVPIVVGGAVGVSPT
                     QITLLVQYALIIAGISTLIQLYPIGNIGSRLPIIFGVGFTYVPTLVAVGGNYGLASIL
                     GAQLIGGVATVLIGIFIKKIRKFFPNIVAGTVVFTIGLSLYPIAINYMAGGVNSPTYA
                     SMQNWAVAAITLVVVIGLSQFAKGYAKLAAIFLGIIVGYCVSLVLGIINFDPIREATW
                     FAIPKPLEFGMEFDMPVIISVVIVSIINAVQAIGDLSATTMGGMDRNITDKELSSGVI
                     GSGICTMIGALFGGLPPSSYSQNVGMVAMNKVISRFVLGIAGIFMLLSGFVPKFGALM
                     TTIPYSVLGGATISIFSMITMTGIKLIITEELSSRNITIVGLAIALGMGITSVPAAQE
                     AFPSFVKLVFGESPIVIATLVAFVLNLIIPNKSLEDEAKERAVMEETN"
     gene            3201..4562
                     /locus_tag="KRL67_002671"
     CDS             3201..4562
                     /locus_tag="KRL67_002671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine/pyrimidine permease"
                     /protein_id="HBH1523537.1"
                     /translation="MKTRNTSKYDVDGIPPLKESIPLALQHLLAMIVGNMVPAILIAN
                     VVGLNQGQATMLIQGSMLAAGLATFLQLYPIPLFKGFKLGSRLPVMMGMSYVFLGACL
                     SVAAKDGLAALFGAQIAAGVIVFFVGFGVKKIRHIFTPIVSGTIIACMGLGLFATAIK
                     NLAGGEGTQTFGSPINFTVGVIVAFVIIMINKYGKGLVKNSSILIGILVGYAISLILG
                     LVDFSAVQGAAIVSLPTPAAFGLEFRPELIVMFTIIYIIGIADMMGACTIATVGAMDR
                     EVTDEELASVVLGNSITSIISSLFAALPTGVFSQNTVIVSMNKVTSRFVIALGALVLL
                     LAGISPALGAIMTTIPSCVVGGATLVVFSSIAMSGFSIMSMDGFTEENNLIAGVSIAT
                     SMGLTTAPQVLDQFPETIGTVLGGSSIVSGAIIAILLQTLFKLKSRKSAENVTSNLEE
                     NIG"
     gene            4635..6029
                     /locus_tag="KRL67_002672"
     CDS             4635..6029
                     /locus_tag="KRL67_002672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HBH1523538.1"
                     /translation="MIDILIKNAIIVTVNKDRDVIFDGAIAIQGDRILDIGNTVDIEP
                     KYLNAKKVIDANGKVIFPGFINTHNHLFQVLLKGLGDDMALHEWLNTMMFPSAKFLTE
                     QDTYDAAMLGCMEGLKSGITTMVDYMHTHNRPGLTDGIVKAYKDLGIRGVVGRGCIDL
                     GIHKELIEDVETVEKDLRRVFEKYHNSENGRIKICVAPSSMWAISEEMGKMLWNIVKE
                     YDSYFTVHISETEFARTATKDIHGEIDIKLLEKWGIVGPEVVAVHCVCITDEDMEMLK
                     KYDIKVSHNVASNMYLASGVAPVPEMLKKGINVSLGLDGAASNNAQDMVELMKLTALQ
                     HKVNNCDPLAISAEKVLEMATIEGARTLRMEDEIGSLEIGKKADLVIFNSMLSPKSIP
                     LHNPVSTLVYSASMHNVESVLVDGNIILENGKVTTIESEEQVLLNAQHAAEELCKRAN
                     VTNRMEGHKWNSLY"
     gene            6091..6561
                     /locus_tag="KRL67_002673"
     CDS             6091..6561
                     /locus_tag="KRL67_002673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HBH1523539.1"
                     /translation="MEKIKLNINDYMYEIEVEKNETLLHVLREKLNLTGAKQGCNSGS
                     CGACKVLIDGVDMKSCKLLARNCEGKVIYTIESFANGENLHPIQEAFIEVGAVQCGYC
                     TPGMIITTQALLNRNASPNEDEIREAFKENLCRCTGYVKIIEAVKLSAARLGGR"
     gene            6564..8780
                     /locus_tag="KRL67_002674"
     CDS             6564..8780
                     /locus_tag="KRL67_002674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HBH1523540.1"
                     /translation="MEKVIGKSYPLKDAALKVTGQMKYVADMKPQNVLYAKMLLSPVA
                     HAKIKSIDTSEAEKVVGVRAIATYLNTSQRKYNGSLRFYEHNIPENEVIFSDTVRFVG
                     DRVAAVAAETPEIAEKAVKLIKVEYEELPAVFTIQDAIKEDAICIHGDSNIVSQNNIN
                     GGDVEKGFKECDYIFEDEYSTPAIHHSAIETHSVIAHYDYNDKLTVHTPCQNTFGFRI
                     ILSQIFGMSLNKVRVIRPAIGGSFGGKLELTIEPVAAALSKMTRRPVKMVLTRKESMI
                     STRTRHGSFVKLKTGVNKDGEVIAQDIKIYTNTGAYASSALNVIGALSHKVFKVYKIP
                     NIKFTGMPVYTNTPIAGAMRGYGSPQIFMAQQAQFAKIAKEIGMDLVDFQNKNAVEPD
                     DVDIIFHGSLGNPRVLDCIEQGKKIFKWDEKKKLPKEEGKYLRGIGMAIGAHGNGVFG
                     AHRDVVTLTLKLNEDGTLTLLTGAHDMGNGVVTMQTMMVAEVLGITPDKVDTFETDTD
                     ACTFNLGDYASRGVFVEGGGAKKTAEKLKNLILEEAEKLLEISKEDLYLDNGYVISKL
                     NENVKASLSDVAVYSQSKSLRELTVVEDYSSPAGRTSYGVHFAEVLVDKETKDIKVVD
                     FVAVHDVGRVINPLNLEGQLEGGIHMGLGYALSEELAFDEQGRPKATDFKTYKLFRTV
                     DMPKCQVAFVNGYEKAGPFGGKSIGECAVVPVAPAVANAVYNATDVEIHSLPIKLK"
     gene            8863..9534
                     /locus_tag="KRL67_002675"
     CDS             8863..9534
                     /locus_tag="KRL67_002675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HBH1523541.1"
                     /translation="MAKILGICGSPRKGATEYAVLEALKEAETIPGIETEYWSVRGKK
                     ISPCVHCDACIRKETMCIIKDDIQELEQKILEADGIIVGSPVYDMNITAQLTAVFNRL
                     RPMYLVHPGKLQNKVGAAITTGGTRYGGQELTKLPIINFFLMHEMLVSGGLGGCYIGG
                     TIWSKDGKAKGAQEDETGMDTVKRLGKGAAEAVMVSKFGLEKWNVVKEELDVKKDEKS
                     PLRDH"
     gene            9897..11111
                     /gene="dpaL"
                     /locus_tag="KRL67_002676"
     CDS             9897..11111
                     /gene="dpaL"
                     /locus_tag="KRL67_002676"
                     /EC_number="4.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopropionate ammonia-lyase"
                     /protein_id="HBH1523542.1"
                     /translation="MLKEIKWKVNNLPKGDKENCIKFLNEEEITKVRNFHKSFPQYKE
                     TPLANLEGLAKKLGVAGVYVKDESYRFGLNAFKVLGGSYSMGRYLAQRLDTDISELGY
                     DKLTSKEIKEKLGEITFFTATDGNHGRGVAWTANKLGQKSVVLMPKGSSEFRLNKIKG
                     EGADASITDLNYDDAVRLANDYAEADDHGVMVQDTAWHGYEEIPAWIMQGYGTMAQEA
                     IEQLKEYGVDRPTHVFVQAGVGSLAGAVQGYVASIYDECPITVVVEADEADCYYKSAE
                     AGDGKPRFVGGDMPTIMAGLACGEPNTIGFEVLKNHAAAFVSAPDWVSAKGMRTLGNP
                     LNGDEKVISGESGAVTTGLLVAAMEREDLADLRKDLKLDENSRILLISTEGDTDPDKY
                     RSIVWDGEYPSI"
     gene            11211..>11801
                     /locus_tag="KRL67_002677"
     CDS             11211..>11801
                     /locus_tag="KRL67_002677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HBH1523543.1"
                     /translation="MLNEQRKQEVTEICQKLIQNPSSSGNEEGVVKAIEESFEKLGFD
                     SWSRDRYGNIVGCIKGNKPGKKILFDGHIDTVPVPDASKWSVPPFEGKIVDGKIYGRG
                     TSDMKGQYTAMMSAVAYFAKDTNRDFAGELYVAGVVHEEIFEGVSAREISKAVQPDYV
                     VIGESSELNLKIGQRGRGEIVVETFGKPAHSANPEKG"
BASE COUNT         4466 a         1355 c         2392 g         3588 t
ORIGIN      
        1 tagaggattt ttgtaaaatg aagaaagtat atctatcaaa ttaaaactaa gtagatatac
       61 tttttattaa gttttgtttt aaaataatat tattaaatta taactaagct tttctgttat
      121 gatataatta aatagataat gtgaatgatt attatataga atacttattt ggagttgata
      181 ttttatggac aaacacagtg attcagtaat gtatggtgga atggaagtat ataatatttg
      241 gggaacaaca acagtagact ccataattgg agagtctgac gtaatgcgtg ctcttaagaa
      301 tagaatttgc aaaattggaa atagtgattc tactgtagct ataactggag aaagtggcac
      361 tggaaaagaa ttaatagcaa gagccattca tctcgcagga aacagaggga ataaaacatt
      421 cgttgctata aactgtgctg ctataccaga acctttacta gaaagtgaac tttttggata
      481 tacaaaagga gcttttagtg gagcagattc aaaaggtaaa attggtaagt ttgaattggc
      541 caatggaggg gttgtatttt tagatgagat aggagatatg ccaattcaac tacaatcaaa
      601 gttattgagg gtattacagg aaaagaagtt tgcgagaata ggttcaaatg aactaattga
      661 tatagatgtt agaatcatat cagctactaa tagagattta tttgagttgg ttaaggaaaa
      721 taaatttaga gaagacttat actatagatt gaatgtaata cctttagaag tgcctccact
      781 tagagaaaga ggagacgata tacaaatatt aataaacaat tttatagaca aatattcgag
      841 aaagatgaag aaaaacgtgt accatataga accagatgtg gaacaaatgt tgcttaaata
      901 tccttggcca ggaaatatta gagagttgga aaatactata gaactggcat taaaccttct
      961 tgaggaagat ggaataatac ataaaggcat aataaatagg aagatacttg aatattttaa
     1021 gttaaataat ataaatataa agttacttga ggaaagtgac tatgagataa gtatggaaca
     1081 agacatactt acacttgaag aatttgaaag agcctatata aagaaagtgt taaagtttta
     1141 cggaaatgat actaagtcta aaaaactggc tgccaaaaaa cttggaatat cactagctac
     1201 attgtacaga aaaatagatg tataataatt agacttttta ttctcaattc taaaatttga
     1261 aattataaaa ttgataaaaa acacttggaa aactattttc taagtgtttt tttgtgtaat
     1321 agcattttat tatccaaaaa taaaaaaaat ttaaaagaat ttaaaaaaat aaatgcttta
     1381 ataaattatc aaatactaac aaatataata aaaattatat atgtaaaaca attatgttaa
     1441 aaaattggca ttataattgc ttcatattaa gttgagatga caaaataaaa aaagaaagga
     1501 tgttgtgtga gataataata actgtcatta aaaaatagtt gggagtgata accttgagta
     1561 aatatcaaaa gaatgtttct atgtttgata ttgatggaag gccaccaatt ttaaagtgtt
     1621 tgccactatc aatgcaacat atactagcaa tgatagttgg aactgttaca gtacctatag
     1681 tcgtaggagg agctgtgggt gttagcccta cacaaataac acttttagtg caatatgcat
     1741 taataatagc tggtatatca acacttatac aactatatcc aataggaaat attggttcaa
     1801 gattgcctat aatttttgga gttggtttta cttatgtacc aacacttgta gcagtcggag
     1861 gaaattatgg attagcaagc attttgggtg cacaattaat agggggagtg gctacagtat
     1921 taatagggat attcataaaa aagataagaa aattttttcc gaatatagtt gcaggaacag
     1981 tggtttttac aattggatta tctttgtatc ctatagcaat aaattatatg gcaggtggtg
     2041 taaatagtcc tacttatgct tctatgcaaa actgggcagt agcggctata acactagtgg
     2101 tagttatagg tcttagtcaa tttgcaaaag gatatgcaaa attggcagca atatttttgg
     2161 gaattatagt tggatattgt gtatctttag ttttgggtat aataaatttt gaccctatta
     2221 gagaggcaac ttggtttgcg atacctaaac cgcttgaatt tgggatggaa tttgatatgc
     2281 ctgtaataat ctcagtagta attgtaagta taatcaacgc tgttcaagca attggagatt
     2341 tatcagctac tacaatggga ggtatggata gaaatataac agataaggaa ttatcaagtg
     2401 gagtaatagg aagtggaatt tgtactatga taggagcttt gtttggaggt ttaccaccat
     2461 catcatatag ccaaaacgtg gggatggtag ctatgaacaa agtaataagt agatttgtgc
     2521 ttggaatagc tggtatattt atgttattgt cggggtttgt acctaaattt ggtgcattaa
     2581 tgactactat accatattct gttttaggag gagctacaat atctatattt agtatgataa
     2641 caatgacagg tataaagtta attattacag aagagttgtc atcaagaaat attaccattg
     2701 ttggtttggc aattgcctta ggcatgggaa taacatcggt cccagcagcc caagaggcat
     2761 ttccttcatt tgtaaagtta gtatttggag aatctccaat agtaattgca acattggttg
     2821 cattcgtttt aaatctaata ataccaaata aaagtctaga ggatgaagct aaagaaagag
     2881 ctgttatgga agagactaat taagatataa taatttgaaa gaattgttta aattatttag
     2941 gatgagattg ttagattatt tttgatagct attcatttta ataaaagatt taacaaataa
     3001 atatatatca ggcaattaat gttcatttaa gttgaaaatc gggggaggga atatttagaa
     3061 atttgttaat tttttataaa aatagctcag tttagctgtg tttatcggtt gagcaataaa
     3121 ttcatggtca taaaaatatt tatagaaaat aattataaaa atatttagtc aagatattta
     3181 tgtatgggag gatttatttt atgaaaacac gtaatacttc aaaatatgat gtcgatggga
     3241 taccaccact aaaggaatca atacctttgg ccttacaaca tctgttagca atgatagtag
     3301 gaaatatggt acctgcgata ttaatagcaa acgtggtagg acttaaccag gggcaagcaa
     3361 caatgttgat tcagggttct atgttagcag caggtttagc aacgttttta cagttatatc
     3421 caataccttt atttaaagga tttaagttag gttcaagatt acctgtaatg atgggaatga
     3481 gttatgtatt tttaggagct tgtctttcag tagcagctaa agatggttta gcagctctat
     3541 ttggggcaca gattgctgct ggggtaatcg tattctttgt aggatttgga gttaagaaaa
     3601 ttagacatat attcactcca atagtttctg gaactataat agcatgtatg ggacttggat
     3661 tattcgcaac agctataaag aatttagctg gaggagaagg tacacaaaca tttggaagcc
     3721 caataaactt cacagtagga gttatagttg cttttgtaat aataatgata aataaatacg
     3781 gtaaaggttt agtaaaaaat tcttcaatat taataggaat attagtaggt tatgctatat
     3841 ctttaatttt aggattggtt gatttctcag ccgttcaagg agcagctata gtatctttac
     3901 caacaccagc agcttttgga ttggaattta gaccagagtt aatagtaatg tttactatta
     3961 tttacataat aggtatagct gatatgatgg gtgcttgtac aatagctaca gtaggagcaa
     4021 tggatagaga agtaactgac gaagaattag catcagttgt tttaggaaat tctataactt
     4081 ctataatttc atcactattt gcagctcttc caactggagt atttagccaa aatacagtaa
     4141 ttgtatctat gaacaaagtt acaagtagat ttgtaatagc actaggggcg cttgtattat
     4201 tactagcagg gatatctcca gcattgggtg caataatgac aactatacca tcttgtgttg
     4261 ttggtggagc aactttagta gttttctcat caatagcaat gtctggtttc tctattatga
     4321 gtatggatgg attcactgaa gaaaacaatc ttatagcagg tgtatctata gcaacgagta
     4381 tgggtcttac aactgctcca caagttttag accaattccc agaaacaatt ggaactgtcc
     4441 ttggaggatc aagcatagtt tctggagcaa taatagctat attacttcaa acattattta
     4501 aattaaagtc acgtaaaagt gctgaaaacg ttacttcaaa tcttgaggaa aatattggat
     4561 aaatgtatta gatataatat gagaaaacca tatggtaaaa tgttgtaatg aaagtattca
     4621 aagggggatt taagatgatt gatatactaa taaaaaatgc aataattgtt acagttaata
     4681 aagacagaga tgttatattt gatggagcaa tagctattca aggtgacaga atcttggaca
     4741 taggaaatac agttgatatt gagcctaagt atcttaatgc taaaaaagtt atagacgcta
     4801 atggaaaagt aatatttcct ggctttataa atactcataa tcacctgttc caagtgttat
     4861 taaaaggttt aggtgatgat atggcacttc atgagtggtt aaatacaatg atgtttccat
     4921 cagcaaaatt cttaacagaa caagatactt atgatgctgc aatgcttgga tgtatggaag
     4981 gtttaaaaag tggtataact acaatggttg attatatgca tactcacaat agaccaggac
     5041 ttacagatgg aatagtaaag gcatataaag atttaggtat aagaggggtt gtaggtagag
     5101 gttgtataga tttaggtatt cacaaagagc ttatagaaga tgtggaaacg gttgaaaaag
     5161 atttgagaag ggtattcgaa aaatatcata attcagaaaa tggaagaata aaaatctgtg
     5221 tagctccttc ttcaatgtgg gctatatctg aagaaatggg taagatgctt tggaatatag
     5281 ttaaagaata tgattcatat tttacagttc atatatctga aacagaattt gcaagaacag
     5341 caacaaagga tatacatgga gaaatagata taaaattact tgaaaaatgg ggtatagtag
     5401 gtccagaagt tgtagcagta cactgtgtgt gcataactga tgaagacatg gaaatgctta
     5461 aaaagtatga tataaaagtt tctcataatg tagcaagtaa tatgtatctt gcttctggtg
     5521 ttgcacctgt tccagaaatg ttgaaaaaag gtataaatgt aagcttaggt cttgatgggg
     5581 cagcaagtaa taatgcacaa gatatggtag agcttatgaa actaacagct ttacaacata
     5641 aagtaaataa ttgtgaccct cttgcaattt ctgcagagaa agttcttgaa atggctacaa
     5701 tcgagggagc aagaactctt agaatggaag atgaaatagg ttctttggag attggaaaaa
     5761 aagctgactt ggtaatattt aactcaatgc tttctccaaa atcaatacct cttcacaatc
     5821 ctgtatcaac tttggtatac tcagctagta tgcacaatgt agagagtgtt ctagttgatg
     5881 gaaatataat acttgaaaat ggaaaagtaa ctacaattga aagtgaagaa caagtgttac
     5941 ttaatgcaca acatgcagca gaagaattat gtaagagagc taatgtaact aatagaatgg
     6001 aaggacataa gtggaatagc ttatactaat attaaaataa gattgtaata atattctatt
     6061 acaacattag tttatctgta ggaggagaaa atggaaaaga taaaattaaa tattaatgat
     6121 tacatgtatg aaatagaagt tgaaaagaat gaaactcttt tgcatgtact tcgtgaaaaa
     6181 ttaaatctaa ctggagcgaa acaaggttgt aatagtggca gttgtggagc atgtaaagtt
     6241 ttgatagatg gagttgacat gaaatcttgt aaacttcttg caagaaactg cgaaggtaaa
     6301 gttatataca ctatagaaag ttttgctaat ggagaaaatc ttcatccaat acaagaagct
     6361 tttatagaag ttggagctgt acaatgtggg tattgtactc ctggcatgat tataacaaca
     6421 caagctcttt taaatagaaa tgcatctcct aatgaagatg aaattagaga agcttttaaa
     6481 gaaaatctct gtagatgcac aggatatgta aagattatag aagctgtaaa attatctgca
     6541 gctagattag ggggtagata attatggaaa aggtaatagg aaaatcatac cctctaaaag
     6601 atgcagcttt aaaagttact ggtcaaatga aatacgtggc tgatatgaaa ccacaaaatg
     6661 tactttacgc taaaatgctt ttaagtcctg tagcacatgc aaaaataaaa agtatagata
     6721 cgagtgaagc tgagaaagta gtaggtgtta gagctatagc tacttatctc aatacatcac
     6781 aaagaaaata caatggttca ttaagatttt atgagcacaa tatacctgaa aatgaagtta
     6841 tattttcaga tacagttaga tttgttggtg accgtgttgc agcagtagca gcagaaactc
     6901 cagaaatagc agagaaagct gtaaaactta taaaagttga atatgaagaa ttgccagctg
     6961 tatttactat acaagatgca ataaaagaag acgctatctg catacatgga gattcaaata
     7021 tagtttctca aaacaacata aatgggggag atgtagaaaa aggcttcaag gaatgtgact
     7081 atatatttga agatgaatac tctactccag caatacatca tagtgcaata gagactcata
     7141 gtgtaattgc acactatgat tataatgata aattaactgt acatacacct tgccaaaata
     7201 catttggatt tagaataata ctgtctcaaa tttttgggat gtcattaaat aaagtaagag
     7261 ttataagacc tgctattgga ggttcatttg gtggaaaatt agaacttaca attgaaccag
     7321 tagcagccgc attatcaaaa atgacaagaa gaccagttaa aatggttctt acaagaaagg
     7381 aatctatgat atcaacaaga actcgtcatg ggtcttttgt aaaattaaaa actggtgtaa
     7441 ataaagatgg tgaagtaata gcacaagata taaaaatata tacaaataca ggagcatatg
     7501 catcaagtgc tttaaatgtt ataggagcac ttagtcataa agtatttaaa gtttataaaa
     7561 taccaaatat aaagtttact gggatgcctg tatatacgaa tacaccaata gcaggagcta
     7621 tgagaggata tgggtcacct caaatattta tggctcaaca ggctcaattt gcgaaaatag
     7681 ccaaggaaat aggtatggat ttagtcgatt ttcaaaataa aaatgcagta gagccagatg
     7741 atgtcgatat aatcttccac ggttctcttg gaaatcctag agtattggat tgtatagagc
     7801 aaggtaagaa gatatttaag tgggatgaaa agaaaaaact tccaaaagaa gaaggaaaat
     7861 atttaagagg tataggtatg gcaataggtg cacatggtaa tggagttttt ggtgcacata
     7921 gagatgttgt tacattgaca cttaaattaa atgaagatgg tacattgaca ctactaacag
     7981 gagcacatga tatgggtaat ggtgttgtaa ctatgcaaac aatgatggta gctgaagttt
     8041 taggaataac acctgataaa gttgatacat ttgaaactga tacagatgca tgtacattca
     8101 accttggtga ctatgcaagt agaggggtat ttgtagaagg tggaggagct aaaaagacag
     8161 cagagaaatt gaaaaacctt atattagaag aagcagaaaa attacttgaa atatcaaaag
     8221 aagatttata tttagacaat ggatatgtaa ttagtaaact taatgaaaat gtaaaagcat
     8281 cactttctga tgtcgcagta tatagtcaaa gtaaatcatt aagagagtta actgttgttg
     8341 aagattattc ttcaccagct ggacgtactt cttatggtgt acattttgct gaagttttag
     8401 tagataaaga aactaaggat ataaaagtag ttgattttgt agctgtacat gatgttggta
     8461 gagttataaa ccctctaaat ttagaaggac aattagaagg tgggattcat atgggtctag
     8521 gatacgcatt gagtgaagag ttggcatttg atgaacaagg tagacctaaa gctactgatt
     8581 ttaaaacata taaattattt agaactgtag atatgccaaa gtgtcaagtt gcatttgtaa
     8641 atggttatga aaaagctggc ccttttggtg gtaagagtat tggagaatgt gctgttgtac
     8701 cagttgcacc agctgttgca aatgcagtat ataatgcaac tgatgtagaa atacattcat
     8761 tgccaataaa attaaaatag tagaaattaa agcacaaata acaatttatt gtatttgtaa
     8821 atagataata aaaatagatt tatttggtga ggagatatta ttatggcaaa gatattaggt
     8881 atttgtggaa gtccacgtaa aggagcaaca gaatatgcag tgttagaagc tttgaaagaa
     8941 gcagaaacta tacctggtat agaaactgag tattggtcag ttagagggaa aaaaataagt
     9001 ccttgtgtac attgtgatgc atgtataaga aaagaaacta tgtgtattat aaaagatgat
     9061 atccaagaat tagagcaaaa aatactagag gccgatggaa ttatagtagg ttctccagtt
     9121 tatgatatga atataactgc tcaacttaca gcagtattta atagacttcg tccaatgtat
     9181 ttagttcatc caggaaaact tcaaaataag gttggtgcag ctataactac tggtggaact
     9241 agatatggtg gccaagaatt gacaaaactt cctataataa atttcttcct aatgcatgaa
     9301 atgttagtat ctggaggttt aggtggatgc tatataggtg gtacaatatg gagtaaagat
     9361 ggaaaagcaa aaggtgccca agaggatgag acaggtatgg acacagtaaa aagacttgga
     9421 aaaggtgcag cagaggcagt tatggtttct aagtttggtc ttgaaaaatg gaatgtcgta
     9481 aaagaagaat tagatgttaa aaaagatgaa aaatcacctc ttagagacca ctaaagattt
     9541 attatattat tgaatatata caaatagtga tgtaatagat tattgcatca ctattttatg
     9601 ttttaattta gattttataa attttatatt aaaaaatata gaaacaaagt gttttgagat
     9661 ttatttttaa attttaaaaa tgagaattaa aaagtgagaa gatagaaaag gttttttagt
     9721 tattaaattc tacatacatt gtatgaacta aagatagaaa agaaaaaata ataaaagcat
     9781 tgcaatttct atactaaaga gtcatgaaaa aatatatagt atatatttag tgttggcatg
     9841 ttaattgctt atatattaaa gtataaaaat ttaacaataa tttggaggta ttttagatgt
     9901 taaaagaaat taaatggaaa gtaaataatt tgccaaaggg cgataaggaa aattgtatca
     9961 aatttttaaa tgaagaagaa ataactaagg ttagaaattt tcacaaaagc tttcctcaat
    10021 ataaagaaac tccattagca aacctagaag gtttagcaaa aaaattaggt gttgcaggag
    10081 tatatgtaaa agatgaatct tatagatttg gattaaatgc atttaaagta ttaggtggtt
    10141 cttattctat gggtagatat ttagctcaaa gattagatac agatatatct gagttagggt
    10201 atgacaaatt aacttcaaaa gaaataaaag aaaaattagg agaaataact ttctttacag
    10261 ccacagatgg aaatcacggt agaggagttg cttggacagc taataaatta ggtcaaaaat
    10321 ctgttgtatt aatgccaaaa ggttcttctg aatttagatt aaataaaatt aaaggagaag
    10381 gtgcagatgc tagtataaca gacttaaact atgatgatgc agtaagatta gctaatgatt
    10441 atgcagaagc tgatgaccat ggagtaatgg ttcaagatac agcatggcac ggatatgaag
    10501 aaataccagc gtggataatg caaggatatg gaactatggc tcaagaagct atagagcaat
    10561 taaaagaata tggagtagat agaccaacac acgtatttgt tcaagcagga gttggttcac
    10621 ttgctggtgc agttcaaggg tatgttgctt cgatatatga tgaatgtcca ataacagtag
    10681 tagtagaggc tgatgaagct gattgttact ataaatcagc agaagctgga gatggaaaac
    10741 caagatttgt tggaggagat atgccaacta taatggcagg gcttgcttgt ggagagccaa
    10801 atactatagg atttgaagta ttaaaaaatc atgcagcagc atttgtttca gcaccagact
    10861 gggtatctgc taaaggtatg agaacattag gtaatccttt aaatggagat gaaaaggtga
    10921 tttctggaga gtctggagca gttacaactg gtcttttagt agcagctatg gaaagagaag
    10981 atttagcaga tttaagaaaa gatttaaaat tagatgaaaa ttcaagaata cttttaataa
    11041 gcacagaagg agatactgac cctgacaaat atagaagtat agtttgggat ggagaatatc
    11101 caagtatata aggaattact aaaatgcaat tattagatac tggtttacat atttagaaaa
    11161 tatatagtat ataaaataca tacattataa aaatctaggg ggataatgaa atgttaaatg
    11221 aacaaagaaa acaagaagta actgaaatat gccaaaaatt aattcaaaat cctagttctt
    11281 ctggaaatga agaaggtgtt gtaaaagcaa tagaagagtc ttttgaaaaa ttaggttttg
    11341 atagttggtc aagagataga tatggaaata tagtaggatg tataaaaggt aacaagccag
    11401 gtaagaaaat attatttgat ggacatatag atacagttcc agttccagat gcaagtaaat
    11461 ggagtgttcc tccttttgaa ggaaaaatag ttgatggaaa gatatatggt agaggaactt
    11521 ctgacatgaa aggtcaatat actgcaatga tgtctgctgt agcgtatttt gctaaagaca
    11581 ctaatagaga ttttgctgga gaactatatg tagcaggtgt tgttcatgaa gaaatatttg
    11641 aaggagtttc agctagagag ataagtaaag ctgttcaacc tgactatgtt gtaataggtg
    11701 agtcttcaga gttaaactta aagattggtc agagaggtag aggagaaata gtagtagaaa
    11761 cttttggtaa accagctcac tctgctaatc cagaaaaagg t
//
LOCUS       DAEPXF010000118        11742 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain HB27
            SAMN13376389-rid12479423.denovo.124, whole genome shotgun sequence.
ACCESSION   DAEPXF010000118 DAEPXF010000000
VERSION     DAEPXF010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13376389
            Sequence Read Archive: SRR10518432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 11742)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11742)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001069624.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001069624.1
            Genome Coverage       :: 315x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/15/2021 01:54:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,165
            CDSs (total)                      :: 4,076
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 89
            rRNAs                             :: 10, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 10 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 19 of 69
            Pseudo Genes (incomplete)         :: 44 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 10 of 69
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11742
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13376389-rid12479423.denovo.124"
                     /strain="HB27"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="China: Hebei"
                     /collection_date="2017"
     gene            complement(686..937)
                     /locus_tag="KRL67_002678"
     CDS             complement(686..937)
                     /locus_tag="KRL67_002678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH1523544.1"
                     /translation="MSSYFITRGDVMKILQQFKPYKYDKEKNIEYDEKIIANQNTEVY
                     IVYPRISDEENRRRWEEFNEVAKEVAINIAMKKVETVES"
     gene            complement(1183..1338)
                     /locus_tag="KRL67_002679"
     CDS             complement(1183..1338)
                     /locus_tag="KRL67_002679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="regulator"
                     /protein_id="HBH1523545.1"
                     /translation="MTLEVKIKVKIIKDVVILNITHWKKETSLLTVKIARSQGDLSYV
                     KYFANES"
     gene            2028..2252
                     /locus_tag="KRL67_002680"
     CDS             2028..2252
                     /locus_tag="KRL67_002680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBH1523546.1"
                     /translation="MDVNQRIKYIRENMNSSQKELLEKTNINTSIMNRIESGERPTRD
                     DGLIIFAKIFGLYTDYILGLSDTEKLNIGN"
     gene            complement(3581..5008)
                     /locus_tag="KRL67_002681"
     CDS             complement(3581..5008)
                     /locus_tag="KRL67_002681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp26"
                     /protein_id="HBH1523547.1"
                     /translation="MKIKKLLTIGLSLSMFIASCPISANALDKIESIKGADKYETAGI
                     IADKQNYTTAILINADSTMADGLSASGLAGAINAPILLTKKNNIPNATLKRLEKAKKV
                     YIIGGENSIDKYTETVLKGKGIEIKRLQGSDRIKTSYNVAKEINSINKVNKVILTNAF
                     KGEPDAMSAAPVAVRDKAAIVLTDGKSVPFNTTGVESYVIGGTSSMNDKLVKDTNSTR
                     LGGTDRYDTNKKIINKFYSGVKEFYIASGTDLVYALVGSTVAKNNAIVLVDNDSNKSV
                     LKSTTKLTAIGNLSDSILQQCLNVTKNIGDSNTGREYTVEDAANAVKKLLGVKKFYYE
                     PNYSEDEDLIFLLDKNFDFSGKEYYVFAYIKQGAEGDVRYCVAKNDMSKVYTYDVNGN
                     MKVCNPDDYKNKGSITEEQAKQIALKECAKRHKVSINNLVVDMIDFRDDIYSGNVYAV
                     KINSKNSNGTFGWFFIDIETGKIYN"
     gene            5199..6020
                     /locus_tag="KRL67_002682"
     CDS             5199..6020
                     /locus_tag="KRL67_002682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HBH1523548.1"
                     /translation="MIISKKITAILVSSLIMISMVGCVPKESATEVAKKYLDSIEIKD
                     YEVAYNLLSKDSKESVSLEKFEEYQDTLFKLQTLKDYKLGEENKVEKYKYSNKEYTDL
                     VKIKETDTIKYITENKEDTANFDRYFIIEDNEYKLLWHKDFKRALSSNYVKIARSETK
                     KDEDNDYNKAAIDIKKAIEIDDKNPPAYYTQSYIYIELQRYDEALKSIDKSLEYLDKN
                     NEAYKIDASDAYNLRGVIFMSKSEFNKARESYNKALELNPDNEYAKTNLNDIKGL"
     gene            6145..7662
                     /locus_tag="KRL67_002683"
     CDS             6145..7662
                     /locus_tag="KRL67_002683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HBH1523549.1"
                     /translation="MSVAIYLRKSRADEDAEKQGEFETLSRHKSTLLKLAKEQNLDVI
                     EIKEELVSGESIIHRPKMLELLKEVEENKYDAVLVMDLDRLGRGDMKDQGIILETFKE
                     SKTKIITPRKTYDLTDEFDEEYSEFEAFMARKELKLISRRMQRGRIKSVEEGNFIGTS
                     APFGYDAVTTGRKERILVPNKDADVVRTIFDLYINEDMGCSKISKYLNNLGIKTATGA
                     NWYNSAITNIIKNKVYCGYIQWQKKDYKKSKNPNKIKTVKLRPKDEWIEAKGKHEPLI
                     SEITWKKAQNILKKNGHVSYGNQIKNPLAGIVICKNCGRPLVYRPYADHDYIICYHPG
                     CNKSSRFEFIEAAILKSLEDTVKKYQLKSSDLDLDKNNKDSNIEFQKRVLKGLETELK
                     ELGKQKNKLYDLLERGIYDEDTFIERSNNISSRTEEIKDSIKTVKNRLSTVKKDNSKI
                     IEDIKTVLSLYHDSDSLGKNKLLKSVIDKAVYYKSKEQKLDSFELMVHLKLHEEQ"
     gene            7685..7969
                     /locus_tag="KRL67_002684"
     CDS             7685..7969
                     /locus_tag="KRL67_002684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012063608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HBH1523550.1"
                     /translation="MCNEISLLDILGTEANYVLDEVELKVQVGKLYEQLNKILTPRER
                     EIVQLRYGLTPYGYKTQREIAQKLDISRSYVSRIEKKALKKLEKELVQES"
     gene            complement(8066..9730)
                     /locus_tag="KRL67_002685"
     CDS             complement(8066..9730)
                     /locus_tag="KRL67_002685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HBH1523551.1"
                     /translation="MSKKKTPFLKKVGKRSWCIFTIILIIYSALIYRLVDIQVLKGDK
                     YKQSVESQSVEKVELNSGRGIIYDRNNKKLTDTSKSQVLVVEKEKLNNSYKILELIKK
                     ATKMNDLDIYKAVQEQLTRPIIQIQTKNIDKSMKKELEKNGIMVEEKTMRYAKDGLLS
                     HTIGYIKEDDKSGQSGIEKSMDSVLRNSNEKYISAFKAGDAGNEKSLNILKGSVKTVD
                     NKDKDRHLKTTIDYNIQKKLEQILNKEENPTAAIISEVSTGEILAMCSRPNFDQNDIS
                     KSLKGKNGEFENRVIKATYPPGSVFKMVVLFSALENGVIDENYTYNCTGKTKVGNTNE
                     ILRCNKRDGHGFQNLRQAFSNSCNPAFLDIAMKLGKEKILKSAEKLHLFEKVDIGLDE
                     EKIREAPKNISIRNLAIGQENIEFTPLQINQMTQIIANNGTFKPLYLYKSLVDNDMNT
                     IKTYKSSKKEDLISPYVCTQVKEYMKSVSRIGTAKDLKDIEGGCGVKTGTAQSSLNKK
                     AIDHGWITGFYPEERPKYVITVLVEGTQKGNKSATPIFKEICESIK"
     gene            complement(9802..11049)
                     /locus_tag="KRL67_002686"
     CDS             complement(9802..11049)
                     /locus_tag="KRL67_002686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HBH1523552.1"
                     /translation="MELNKVELLAPAGDLERLKIAITYGADAVYIGGEIFGMRSAAKN
                     FSKEDMAEGVAFAHERGKKVFVTVNIIPHNEDFLQLEDYLLELEDIGIDAVIIADPGV
                     LSVIKKVIPNMEIHLSTQANTTNYLSANFWYEHGIKRVVVARELSFDEISEIRAKTPL
                     DMDIEAFMHGAMCISYSGRCLISNYMTGRDANKGSCAQSCRWQYHLVEEKRPGEYFPI
                     YEDERGTFFFNSKDLCMIEYIPELIKSGITSLKIEGRMKTAYYVATVVRAYRMAIDEF
                     YRDPENWKFNPMWMEELKKGSHRHFTSGFYLNKPTTEDQNYQSASYVRNYDFIGIVRE
                     TEDEDGLIVVEQRNKMCVGDEIEVMGPYKETMFTKIEEMYNEEGEAIESAPHPRQIVK
                     LKLSVKVGKDYMLRKVIEEKVEE"
     gene            complement(11036..11710)
                     /locus_tag="KRL67_002687"
     CDS             complement(11036..11710)
                     /locus_tag="KRL67_002687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HBH1523553.1"
                     /translation="MSNIVNDLVEDYIRVTLKEKEGFLKDLEIYAEENSVPIIHKEVS
                     DLLKVLLKVQKPKRILEVGCAIGYSSIFFATIIGKDADIITVERNEKMIEKAKENIKL
                     AGFDNNITILEGDAEEKLSQVQGEFDLIFIDAAKGQYKLFFDLVIDKLKDGGLLVSDN
                     ILYKGMVAHDDFVVRRKKTIVKRMRNYLDYICNCDYLSTSLVPIGDGVALSYKERKRG
                     DVDGIK"
BASE COUNT         4040 a         1844 c         1344 g         4514 t
ORIGIN      
        1 attaatacat ttttatatcg cctcttcaat ttgagcccca tcattatata aaattaacct
       61 actggatatc ttaatttaat atatgtacac tattcatatc ttaatagtca atattattca
      121 tttttaatag ctctttttag atgtcagaat tattatattt tgaaatttat taaatttttg
      181 cattaaaaaa actagaaatt aatttaagtc ttgaattact tataactttc tacgtatcta
      241 cctttatatt cttactctga ttttatatct aatattaact cttcatcaaa gctattaaat
      301 atacctctaa ttgagtcaat tatttcactt acttcacctt taatttttat attaacttca
      361 accatttcaa atctcatctt tttatcttat aatttctata agttttttta tttattgtgt
      421 tgcttatttt taccagcact tttttattta atagtctaaa tgatattttt taccagtttt
      481 aatatcctta aatactatat ttactaaaaa cttataattt cttttaatac atagttaata
      541 cattcttatt actaataaca atactcaaca attttatcgc cttttttcaa atatgattta
      601 acttttttat tcctaattat ttaaaaatag ttgtccaatt ttcttctatg agaaagtgct
      661 aagtattagc aacttctcta ttctcttaac tttctactgt ctcaactttc ttcatagcta
      721 tattaatagc tacttcttta gctacttcat tgaactcttc ccatcttctc ctattttctt
      781 catcagatat acgtggatat actatataaa cttctgtgtt ttgattagct ataatttttt
      841 catcatattc tatatttttt tctttatcat atttgtatgg tttaaattgt tgtagtattt
      901 tcataacatc accccttgtt ataaaatatg aactcataaa cttgtcttat acttatttaa
      961 ttgttacaat ttttacttaa aatcacttta tattctatat tttaaagcta aatcctttac
     1021 aaaagtaaca tattcttcta taagtttctt atcctcttat ataatatcta gtttgtttaa
     1081 cttatctctt ttaaacttac atactccttc ttctgccatt cgtcttttgt taattgttaa
     1141 ccttgtttca agagacacac caaaactctt gttaagaagc tcttaactct cattagcaaa
     1201 atattttaca taacttaaat caccttgact tctagctatt ttaactgtta atagacttgt
     1261 ttcttttttc caatgtgtta tatttaatat aacaacatct tttattattt taacttttat
     1321 ttttacctct aatgtcatat tattgcttga ttaatttgta ttcttaaatc ttcaatttat
     1381 cttaaatttt ctattaatac atcttctata caggttagtt tctctcgctt aaccttgaaa
     1441 tatgttttct ctaaattgtc agattgttct caagttttat cagtatctaa tactttgtag
     1501 tgtatatttg aacatctttc ggtccaaagg tataattgac tagaatgttt tgaaatcaac
     1561 tgactattaa tagattagtt cttaaattct ttcaaatact cgaattgtaa taaaacataa
     1621 tttacattct ctttatttct attgaagtta gtttatatat tgtttacatc attttcatat
     1681 atatctgcta gttgctatgt atttaaaatt ctcttattat ttctttatat tacttgtaaa
     1741 ttattcatcc ttatcatcct cttaattaaa ttttctatat cataatcaag tatcttagat
     1801 agtttatagg taatatatca actctaattg aatattaccc tttcctgtac ttaaaataag
     1861 ttgatgcatt tttaaaacct aactcttttg caacactttg taagtataaa ttttatgtat
     1921 gtcttctttt ttataaaatc atagtgtatc tttttcatat attcacatta atttctatat
     1981 ttcaataaca atatttccaa agcagaaact tttcgagata attatgtatg gatgtaaatc
     2041 aaaggattaa atatattagg gaaaatatga atagttctca aaaagaattg cttgaaaaaa
     2101 caaatataaa tacaagtatt atgaatagaa tagagtctgg tgaaagacca acaagagacg
     2161 atgggttaat aatatttgct aaaatttttg gtttatatac tgattatatt cttggtttat
     2221 cagatacaga aaaactaaat attggcaatt aaaactgaat taattgtatt atatgtgtga
     2281 tgaatataaa tagagaattt tttgaaaagc ttgaaataac ttagaaaaaa taagaggttc
     2341 actcaataac aagttgctaa agactaaaaa acaacaaagg tatctactaa aagatatgaa
     2401 aatagtttaa gagagttgag tctggaaata tttatgtgta ttctttaaat aaaaatttat
     2461 ttttaaatga taattaaatt tttactgata tggctatgct tttaaaagtc ttaaccttat
     2521 tttgatatta tatctgtctt tacataccac ttagttaata taaaacttgt agacttcaac
     2581 cctacaatgt tagtaggttc agtctttaca taccacttag ttaatataaa accccaaaat
     2641 aaattgagta cttccaacac ttacatctat acaattctct caaatttgca gtgaaccacg
     2701 agtagtgcaa atgataatat ttatcatata ccctcaatgc cttatgtttc aactgttaag
     2761 ccctatttta tcaaaaatat cgctcactgc aaaactattt ataatattat gaatatatta
     2821 tttttaaata tctgtaccaa tttgtagtat aataaaatca agaaaataat ttactcctat
     2881 aaagagtgat tatttttaag tgaaaatatt atttttttaa atcattctta ttggcgtttg
     2941 ggagattttt attttatcat aaatataaac tgatataaca ccagctgaca tgcttgccac
     3001 tttagattat tattcttgct acaactatta taacatataa aacttactta tttttaccaa
     3061 atattttatg taaacaataa atattaactt atgaaatatc tgctcattta cagatatttc
     3121 ataagttaat agctctataa taaaattttt atcattgttt ataaaaaatt ttactttgca
     3181 tttttttata aacaacagtt ttatttataa tattttgtgg tataataaaa gcaagaagaa
     3241 ctacaatcta ttttgcgtta gagtggagtt catcataaac agagtttatt ttttggattt
     3301 aaatataaat ttaaattcaa ctcgtaagtc actctttgtg cgagagtggc tttttgcttt
     3361 tttgaatagt ttactgatta agtaaactac cacactagct gttaaactag ctaaaacatt
     3421 aaacaaaaag ttatccatga actcacctcc cttcgaatcg ttgggaggat aatcttttgt
     3481 acatgaactc cactctatag attgtaagtt aaatcttctt gctaaaaata ttataacata
     3541 ttatggtaaa ttatataatt attgtttaaa agttctaatt ttaattataa attttcccag
     3601 tttctatatc tataaaaaac caaccaaaag taccattact attttttgaa ttgattttaa
     3661 cagcatagac attaccacta tatatatcat ctctaaaatc tatcatatca actactaagt
     3721 tattgatact gactttatgt ctcttagcac attcttttaa tgctatctgt tttgcttgtt
     3781 cctctgttat acttccttta tttttataat catctggatt acatactttc atattaccat
     3841 taacatcata tgtataaact ttgctcatat catttttagc tacacagtat ctaacatcac
     3901 cctctgcacc ctgtttaatg taagcaaata catagtactc ttttcctgaa aaatcaaaat
     3961 ttttatctag tagaaatatt aaatcttcat cttctgaata attaggttcg taataaaatt
     4021 tcttcacacc taatagtttt ttcactgcat tagcagcatc ttctactgta tattctcttc
     4081 cagtattaga atcgcctata ttttttgtta catttaaaca ctgctgtaat atactatcac
     4141 tcaaatttcc tatagcagtt aatttagttg tacttttcaa tacactttta ttactatcat
     4201 tatcaactaa cacaatagca ttatttttag caacggtaga accaacaaga gcatacacta
     4261 aatcagtacc acttgctata taaaattctt ttactccact ataaaattta tttattatct
     4321 ttttgttagt atcatatcta tctgtcccac ctaatctagt tgaattagta tccttaacta
     4381 gtttgtcatt cattgatgaa gtaccaccta ttacataact ttctacacca gttgtattaa
     4441 atggaacact ttttccatca gttaatacta tagctgcttt atctctaact gctacaggtg
     4501 cagcactcat tgcatcaggc tctcctttaa aagcattagt caatatgact ttatttactt
     4561 tgttaattga gtttatttct tttgcgacat tatagcttgt cttaattcta tcactacctt
     4621 gaagtctttt aatctctatt cctttgcctt ttagaacagt ttcagtatat ttatcaatag
     4681 agttttctcc tccaattata tatacttttt ttgccttttc taatctttta agtgtagcat
     4741 ttggtatgtt atttttctta gtcaaaagaa ttggagcgtt tatagctcct gcaagtccac
     4801 ttgcacttaa tccatcagcc attgtactgt ctgcattaat taatatagct gtagtatagt
     4861 tttgtttgtc tgcaattata ccagcagttt cgtatttatc tgcaccttta attgattcaa
     4921 ttttatcaag agcatttgca gatatagggc aactagcaat aaacatagat aaagataacc
     4981 ctattgttaa taatttttta attttcataa tatctccttc tcttaaatat tttcacctaa
     5041 attatataac acttttcagg aaaatgttac cataaatttt aaaaattatt attacttaat
     5101 ataatattaa tgaaaaatat cattcttaaa ttaatgactt tggtagaata tggtgataaa
     5161 ataaaattag tgagagacaa tataaggggg attatattat gataatcagt aaaaaaataa
     5221 cagcaatatt agtatcatct ttaattatga tatcaatggt tggatgtgtt ccaaaagaaa
     5281 gtgctacaga ggtagctaaa aagtaccttg atagtataga aatcaaagac tacgaagtag
     5341 catataattt attaagtaaa gacagtaagg aaagtgtcag tctagaaaaa tttgaagaat
     5401 atcaagatac tttatttaaa cttcaaaccc taaaagatta taaacttggt gaagaaaata
     5461 aagtagaaaa atacaaatat tctaataaag agtatacaga tcttgttaaa ataaaagaaa
     5521 ctgatactat aaaatatatc actgaaaata aagaagatac tgcaaatttt gatagatatt
     5581 ttataatcga agataatgaa tataaattat tatggcataa agactttaaa agagctctta
     5641 gctctaatta tgtcaaaatc gctaggtcag aaacaaaaaa agatgaagat aatgattata
     5701 ataaagctgc tattgatatc aaaaaagcta tagaaattga tgataaaaat ccacctgcat
     5761 attatacaca atcatatatt tatatagagt tacaacgata tgatgaggct ttaaagtcaa
     5821 ttgataaatc cttagaatat ctagataaaa ataatgaagc ttataaaata gacgctagtg
     5881 atgcttacaa tttaagaggt gttattttta tgagtaaatc agaatttaat aaagctagag
     5941 aatcttacaa taaggcgctt gaacttaacc cagataatga atatgcaaaa actaatctaa
     6001 atgatattaa aggattataa ggcattaata ttatagaaaa taagtatgtt gaaagttaaa
     6061 ttaacttttg acatgcttat tttctatatt ttaaactcta ttgatttact atataatagt
     6121 acctaaggag tgtgattact tattatgagt gttgccatat acttaagaaa atcccgtgct
     6181 gatgaagatg cagaaaagca aggtgaattt gaaactttaa gtagacataa atcaacccta
     6241 ctaaaactag caaaagaaca aaacttagat gtaattgaaa taaaagaaga attagtttct
     6301 ggtgaaagta taatacatag acccaaaatg cttgaacttc taaaagaagt tgaagaaaat
     6361 aaatatgatg cagtcttagt aatggactta gacagacttg gtcgtggaga catgaaagac
     6421 caaggtatca tattagaaac attcaaagaa agtaagacca aaatcatcac acctagaaaa
     6481 acatacgacc taacagatga atttgatgag gagtactcag aatttgaagc ctttatggca
     6541 agaaaagaat taaaacttat atcaagaaga atgcaaagag gtagaataaa atcagttgaa
     6601 gaaggtaatt tcataggaac ttctgctcca tttggatatg atgctgtaac aacaggtaga
     6661 aaagaaagaa tacttgtacc aaataaggat gctgatgtag ttaggactat ctttgactta
     6721 tatattaatg aagatatggg ttgtagtaaa atatcaaaat acctaaataa cttaggcatt
     6781 aaaactgcta caggtgctaa ttggtacaat tctgcaatta caaatataat aaaaaataag
     6841 gtctactgtg gctacattca gtggcaaaaa aaggactaca aaaaatctaa aaatccaaat
     6901 aaaataaaaa cagttaaact aagacctaaa gatgaatgga ttgaggcaaa aggaaagcat
     6961 gaacctttaa taagtgaaat aacatggaaa aaggctcaaa atatactcaa aaaaaatggt
     7021 catgtcagct atggtaatca aataaaaaat ccacttgcag gaatagtgat ttgcaaaaat
     7081 tgtggtagac cattagtata tagaccatat gcagaccatg actatataat ttgttatcat
     7141 cctggttgca ataaaagttc tcgatttgaa tttattgaag ctgccatttt aaaatctttg
     7201 gaagacactg taaaaaaata tcaattaaaa tcatctgatt tagatttaga taaaaataat
     7261 aaagatagta atatagaatt tcaaaaacga gtcttaaagg gtttagaaac tgaattaaaa
     7321 gaattaggca agcaaaaaaa taagttatat gatttactag aacgaggaat atatgatgaa
     7381 gatacattta ttgaaaggtc caacaatata agttcaagaa ctgaagaaat taaagattca
     7441 attaagacag taaaaaatag attgagtact gttaaaaaag acaattctaa aataatagaa
     7501 gatattaaaa ctgtgttatc actttaccat gattcagatt cccttggaaa aaataaactc
     7561 ctaaaatctg taattgataa ggcagtttat tataagtcta aagagcaaaa actagattct
     7621 tttgaattaa tggtccattt gaaattacat gaagaacagt aataccaatg acatataaag
     7681 agtagtgtgt aatgaaataa gtcttttaga tatacttggc acagaggcta actatgttct
     7741 tgatgaagtt gagctaaaag ttcaagttgg aaaactttac gaacaactaa acaaaattct
     7801 tactcctaga gaaagagaaa ttgtccagtt aagatatggg ttaactcctt atggctataa
     7861 gacacaaaga gaaattgcac aaaaactcga tatatcaagg tcatatgtat ctagaatcga
     7921 aaaaaaagct cttaaaaaac tagaaaaaga gcttgttcaa gaaagttaat actatatttt
     7981 aaaaggatat atcgtacatc attagacaaa attttctatc taaaatcaaa gttacgatat
     8041 atccttaaat tttatagtac ataatttatt ttatactttc acatatttcc ttaaatatag
     8101 gtgttgcaga tttatttcct ttttgagttc cttctactaa tacagttatt acatacttag
     8161 gtctttcttc aggataaaat ccagttatcc atccatggtc tattgctttt ttatttaaac
     8221 tactttgagc tgtaccagtt ttaactccac aaccaccctc aatatctttt aggtctttag
     8281 ctgtaccaat cctagataca gacttcatat attctttaac ttgtgtacac acatatggag
     8341 aaataagatc ttcttttttc gaagacttat aagtttttat tgtattcata tcattgtcca
     8401 caagactttt atataagtat agtggtttga atgttccatt attagctatt atttgagtca
     8461 tttgatttat ttgaagtggt gtaaattcta tattttcttg acctatagct aaatttctta
     8521 ttgatatgtt ttttggagct tctctaattt tttcttcatc aagaccaata tcaacctttt
     8581 caaataaatg taatttttca gcagatttga gtatcttttc ttttccaagt ttcatagcta
     8641 tatctaaaaa tgcaggattg catgaattag aaaaagcttg tcttaagttt tggaaaccat
     8701 gaccatctct tttattacat cgcagtattt cattagtatt cccaacttta gtcttacctg
     8761 tacagttgta agtatagttc tcatctataa caccattttc aagtgcagaa aataaaacta
     8821 ccatcttaaa tacagaccct ggtggatatg tcgcctttat aactctattt tcaaattcac
     8881 cattttttcc ttttaaactt tttgatatgt cattttggtc aaaattaggt ctagagcaca
     8941 ttgcaagaat ttcacctgta gatacttccg agattattgc tgctgtagga ttttcttctt
     9001 tatttagtat ttgttctaat tttttttgaa tgttataatc aatggtagtt tttagatgtc
     9061 tatctttatc cttattatca acagttttta cacttccctt tagtatgttt agagattttt
     9121 catttcctgc atctccagct ttaaaagcac ttatatactt ttcatttgaa tttctaagta
     9181 cactatccat actcttttca attcctgatt gacctgattt atcatcttct tttatatatc
     9241 ctattgtgtg tgataataaa ccatcttttg cgtacctcat agttttttct tctaccatta
     9301 ttccattttt ctcaagttct tttttcatac tcttatcgat atttttagtc tgaatctgta
     9361 ttatagggcg tgtcaattgc tcttgtacag ctttatatat atctaaatca ttcatcttag
     9421 tagctttctt aataagctcc aaaattttat agctattatt taatttttct ttttcaacaa
     9481 ccagtacctg agatttactt gtatctgtaa gcttcttgtt gtttctatca tagattattc
     9541 ctcttccact atttaactcg actttctcaa cactttgtga ttcaacactt tgtttgtatt
     9601 tatcaccttt taatacttgt atatcaacta gcctataaat taaagcacta taaattatta
     9661 aaattatagt aaatatacac cagcttctct ttcccacttt tttcaaaaat ggagtcttct
     9721 tttttgacat gaaatcaccc cttagataat ttcattattg acaatttcta aggggctaat
     9781 actattttta tcaatttttt attattcttc aaccttttct tctataactt ttctaagcat
     9841 ataatcttta ccaactttta ctgatagctt taacttcact atttgtctag gatgaggggc
     9901 tgattctata gcttcccctt cttcattgta catttcttca attttagtaa acatagtttc
     9961 tttatatggt cccataactt ctatttcatc accaacacac attttatttc tttgctcaac
    10021 aacaataaga ccatcttcat cttctgtttc tctaactata cctataaaat catagtttct
    10081 aacatatgat gctgattgat aattttggtc ttctgtcgtt ggtttattca agtaaaaacc
    10141 tgatgtgaaa tgtctatgac tacctttttt tagttcttcc atccacattg gattgaactt
    10201 ccagttttct gggtctctgt aaaattcatc tattgccatt ctgtatgctc ttacaacagt
    10261 agctacatag tatgcagtct tcattctacc ttctattttt aaacttgtta tgccagattt
    10321 gattaactct ggtatatact ctatcataca taaatctttt gagttaaaga aaaatgtacc
    10381 cctttcatct tcgtatattg gaaaatactc accaggtctt ttttcttcca ccaaatgata
    10441 ttgccatcta caagattgag cacaagaacc tttgtttgca tctcttccag tcatatagtt
    10501 acttattaaa catctaccag aataagaaat acacatagca ccatgcataa acgcttctat
    10561 atccatatct aaaggtgttt ttgctcttat ctcagatatt tcatcaaaag ataattctct
    10621 tgccactaca actcttttta ttccatgctc gtaccagaag tttgcactta gataattggt
    10681 tgtatttgct tgtgtactta aatgtatttc catattcgga attacttttt taatgacact
    10741 aagcactcct gggtcagcta ttataactgc atctattcct atatcttcta gttccaacaa
    10801 atagtcttct aattgtagaa aatcttcatt atgaggtatt atatttactg ttacgaatac
    10861 tttcttgcct ctttcatgtg caaaagctac tccctctgcc atatcttcct tactaaaatt
    10921 cttcgctgca gacctcattc caaaaatttc tccacctata taaacggcat ctgctccata
    10981 tgttatagct attttcaacc tctccaaatc acctgctgga gctaaaagtt ctactttatt
    11041 taattccatc tacgtcacct ctttttcttt ctttataact tagtgctaca ccatcaccta
    11101 ttggtaccaa tgatgtgctt aaatagtcac aattacatat ataatctaaa taatttctca
    11161 ttcttttaac tatagtcttt tttcttctta caacaaaatc atcatgagca accatacctt
    11221 tatacaaaat attatcagat actaaaagtc ccccatcttt taatttatct ataactaaat
    11281 caaaaaatag cttatattga cctttggctg catctataaa tataaggtca aattcacctt
    11341 ggacttgact aagtttttct tctgcatctc cttctagtat agttatgtta ttgtcaaatc
    11401 cagctaattt tatgttttct tttgcttttt ctatcatttt ttcatttctc tcaacagtta
    11461 ttatatccgc atcttttcct ataatagttg caaaaaaaat agaagaataa ccaattgcac
    11521 atcctacttc taaaattctc tttggttttt gaacctttaa taagactttt aataaatctg
    11581 aaacttcttt atgtattata ggaacactat tttcctctgc ataaatttct aaatctttta
    11641 aaaatccttc tttttctttt aatgtcactc ttatataatc ttcaaccaaa tcattaacta
    11701 tattactcat aaaaacactc cctaaaataa taaatattca ag
//
LOCUS       DAEPXF010000119        11738 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain HB27
            SAMN13376389-rid12479423.denovo.125, whole genome shotgun sequence.
ACCESSION   DAEPXF010000119 DAEPXF010000000
VERSION     DAEPXF010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13376389
            Sequence Read Archive: SRR10518432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 11738)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11738)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001069624.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001069624.1
            Genome Coverage       :: 315x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/15/2021 01:54:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,165
            CDSs (total)                      :: 4,076
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 89
            rRNAs                             :: 10, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 10 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 19 of 69
            Pseudo Genes (incomplete)         :: 44 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 10 of 69
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11738
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13376389-rid12479423.denovo.125"
                     /strain="HB27"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="China: Hebei"
                     /collection_date="2017"
     gene            243..1568
                     /gene="cdeA"
                     /locus_tag="KRL67_002688"
     CDS             243..1568
                     /gene="cdeA"
                     /locus_tag="KRL67_002688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MATE transporter CdeA"
                     /protein_id="HBH1523554.1"
                     /translation="MENLFTRKFTTFEFLKFVSPAIISMIFISLYTIIDGIFVSTLVG
                     SNALASINIVLPIINLVCGFGIMMATGGGAIVSIRMGENRQDEANSTFSFIVLFSLIV
                     GILFTVISYFFIKEISMLLGATDKLLPYCITYGKVMILCTPFYILKFIFEYFARTDGN
                     SKFSLFLSVIGGVTNIILDYVFIKYFGMGLLGAAVATAIGIILTCVLGIIYFLSNKST
                     LKLRKPKTDFRLIRDTMINGSSEMVTELSTGITTFLFNIVALKLAGENGLAALTIVLY
                     AHFLMTSVYLGFAAGVSPLISYNFGAENSDKLKETFKHSLKFIFVSSLLVFIIALVFA
                     PFIVRVFVNPDNTVFKLALQGLKIFAFAFLFVGINIFASGFFTAFHNGKISAIISFSR
                     AFVFIIIGIIILPPMLNLTGLWLTVPFAEVLTLFISILFIKKYKGRYKY"
     gene            complement(1663..2457)
                     /locus_tag="KRL67_002689"
     CDS             complement(1663..2457)
                     /locus_tag="KRL67_002689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBH1523555.1"
                     /translation="MNELKPYIKFFNKNTKLYIIIFMIVGIVMNILPVFILKILGEAS
                     KTNLSTTVNPILTYITILPFVYGVNIANKDFSGALSIRADRKSCIKAIVIDLIILSFV
                     ISILGIVLIFLSKLFIEVMTGYSIELSVLLKRDMIWTSISALIDPSTSSTPLTYLNYF
                     VQICINEICIGFIGVLLGAFTYRVRKVTSITILIGLPILIVIYMVNFATKNMHKMLLY
                     LEKIIYSFQNPFILFGTKVGIIVICIIFIILLLRKAPIKEYANDLL"
     gene            complement(2458..3333)
                     /locus_tag="KRL67_002690"
     CDS             complement(2458..3333)
                     /locus_tag="KRL67_002690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBH1523556.1"
                     /translation="MSSSIKVENLSHSFKKQNIFNNMSLNFEKNKIYGLLGKNGAGKT
                     TLLNIMVNQLIQNEGEIEILGKNPKEDIKVLEEVCIVREKEFYSPDFKVGQIFEFSSS
                     FYKSYDKGLEEKLCKYFDLNTKKKYKQLSRGMKTILSNIIGICSNSAITIFDEPTIGL
                     DAVNRQEFYNIILDSYIKNPRTIIISTHLIDEIDDLLEHVIILHEGKIIIDEEIDIIK
                     QKAHYITGNKEELEKLECIKNMKPKKAFGNTVAYFYYGDFSENDEKILKNSTIDVGYI
                     GLQDMFVNMTKKEEL"
     gene            complement(3337..3705)
                     /locus_tag="KRL67_002691"
     CDS             complement(3337..3705)
                     /locus_tag="KRL67_002691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBH1523557.1"
                     /translation="MKLVLNNEEPIFIQIARAIEDEILSNGIKEEEQVPSTTELSKLY
                     KINPATVLKGINILVDKNILYKKRGIGMFVSDGAKTIIKEARKENFKHNFVKNLLQEA
                     NKLEINREELVDIIINFKED"
     gene            complement(3887..4546)
                     /gene="deoC"
                     /locus_tag="KRL67_002692"
     CDS             complement(3887..4546)
                     /gene="deoC"
                     /locus_tag="KRL67_002692"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HBH1523558.1"
                     /translation="MKHILKTVDHTILKATTTWEDIKILCDEAVDMSVASVCIPPSYV
                     KRASEYLKGKIKICTVIGFPLGYQTTATKVFEAKDAIENGADEVDMVVNISDIKNKDY
                     DNIGKEIKEIKKAIGDKVLKVIIETCYLDEDEKVKMCEIVTMSGSDFIKTSTGMGTGG
                     ATLEDIKLMKEHVGKNVKIKAAGGVKSISDAEKFIEAGAERLGTSSICKILKNENTTD
                     Y"
     gene            complement(4582..6126)
                     /gene="murJ"
                     /locus_tag="KRL67_002693"
     CDS             complement(4582..6126)
                     /gene="murJ"
                     /locus_tag="KRL67_002693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HBH1523559.1"
                     /translation="MSKVAKATFYLMIVTIISKILGMGRELVLSSIYGTGLYTESYLT
                     AMNIPNIIFAAIGTAIVTTFIPMYQDISSKQGEKQALKFLNNVLNIIVGICIVVAILG
                     VIFSKQLVSIFAIGFEGERFLLTVKFTKILITGIIFIGITSVMSAFLQIKDNFIVVGF
                     GSIPYNIVIIISIMLSTVFGPYILPIGAVVAMVVQLLFYMFFVKKTNYKYLYYLNFKD
                     DSLIKLLALLSPVFIGVAVNQVNSLVDTTLASTLVKGSIPALTYADRLNGFVTGTFTA
                     SIVSVMYPMLSKLSAENNQKKFTSSVKSSINMIIISMIPISVASIFFATPVVRIIFER
                     GAFDARATQMTATALIFYAVGMTAFGLRDILGKVFYSLQDTKTPMVNGIISVGVNIVL
                     DLVLIKPMAHGGLALATSSSSIACILLLFLNLKRKVGYFGQDKIIKATLKSVIASLIM
                     GVLSYFTYKFIFGILGVGTFNEFVSLAISVIVGGGVYTLLMTIFKVEEVDMILNIAKR
                     KLHLKK"
     gene            complement(6600..7484)
                     /locus_tag="KRL67_002694"
     CDS             complement(6600..7484)
                     /locus_tag="KRL67_002694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HBH1523560.1"
                     /translation="MDTKKENNYENYICVGSSNVSNAMKMYLKEIEEYKMLSAGEEVE
                     LAKEIINSSSVAKEKFINSNYRLVVSIAKRYKRDSIDMLDLIQAGNIGLIKAVEKYDY
                     KKGYKFSTYATWWIKQSITRYIDDCENTIRIPIHLHQRINFVKKKKQELLNVLLREPT
                     IDEIADACGLEVDKVLELLRRDKNVVSLDTPLKEDEDSSLVEFIPSDADFKDVVIHEV
                     EQHNLKEKIEELLTGLGEQEQQVLRMRFGIDDDDPKTLEQIGKVFGVTRERIRQIEAK
                     AIRKLRHPSKLKQLKHFY"
     gene            complement(7775..8251)
                     /locus_tag="KRL67_002695"
     CDS             complement(7775..8251)
                     /locus_tag="KRL67_002695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction enzyme HsdR N-terminal
                     domain-containing protein"
                     /protein_id="HBH1523561.1"
                     /translation="MSKELVKDRVIKYLIEDLLVPQDMIDTNVELAEFEEGAEGILDI
                     VVNVKDEEDYYAPVMIVQCLDEDVELEGEVLQKQIEFLEEVDNITMSGRLVLTNGDAM
                     MYADWRGEEYDTEAALPTYDIMVKEFHEMEQQVKDLEEHHHHDENCGCGCNHHHEN"
     gene            8518..9693
                     /locus_tag="KRL67_002696"
     CDS             8518..9693
                     /locus_tag="KRL67_002696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1002 domain-containing protein"
                     /protein_id="HBH1523562.1"
                     /translation="MKFKKQISSIVVSTMLVLGSMNLSYADGTNVVTLGANLSESQKQ
                     QMLNYFGVKKDQVLVLDVTNAEERQYLQGVATEGQLGKVTISCSYVEPTKKGNGINVK
                     TANLTWVTSSMIASTLTTAGLEDANVIAAAPYPVSGTGALTGIMKAFEDASGKKLDET
                     KKEIASEELVVTGDLGDDIGQEKATGVMNDIKTEIVKNGTKDTNQIANTINNVVNNYN
                     ITITPEQKEQIKGVMKKIADQNYDYNSMKNTLDNVSDNVNEQLKKLGESVKGSGILDT
                     IGGWFSGIGDWFSGLFSGGDKKDLGILNNTNDESLGSDAVINSTEGVQINPQNNTNSN
                     NDSDTNSSKNDTSSNNENNDSSQTNENNTTNNSENQSNNNDSVGFFEKIKNWLSGLF"
     gene            complement(9774..10577)
                     /gene="proC"
                     /locus_tag="KRL67_002697"
     CDS             complement(9774..10577)
                     /gene="proC"
                     /locus_tag="KRL67_002697"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HBH1523563.1"
                     /translation="MKKIGFIGAGNMASAMIGGIVNSKLVEPNMVIASAYSQETLDRI
                     DANFGINTTKDSKEVTRTSDIVIVAVKPDIYDDILEEIKDLIDDNKIIVTIAAGKSIK
                     DIESIIGEDKKIVRTMPNTPALVNEAMSSLSINKNISNEDLEAVTEVFNSFGNTEVVP
                     EYLIEAVIGASGSAPAYVFLFIEAIADAAVIAGMPRPQAYKFASQAVMGSAKMVLETG
                     KHPGELKDMVCSPGGTTIEAVKILEEEGFRASVIKAICACIEKSKKMSE"
     gene            complement(10617..11201)
                     /locus_tag="KRL67_002698"
     CDS             complement(10617..11201)
                     /locus_tag="KRL67_002698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBH1523564.1"
                     /translation="MNKDIGAKIKQLRTQKQMTLKDMSEKTNLSIGFLSQLERGLTSV
                     ATDSLGKIASVLDVELTYFFMKPKEHKRAVLRSYEKEVFDVENSTFIHYHLSSSLKEK
                     TMLPRLIEILPSKSSEEICCYVHEGEEFVYVLEGTLTVFLGDEQIEMYPGDTIHYNSE
                     KNNHNWVNYTNKVVKILVVSIPNPFEKSDAVKEA"
BASE COUNT         3933 a         1895 c         1296 g         4614 t
ORIGIN      
        1 ttataaaact taagattata tacatatatt tctaaaaatt ttcttaagaa acagttatac
       61 cttactaatg caatatgcct attcttacta atataagttt attaattatc attatactaa
      121 ttcatcttaa tatgattttt tgttgatttt aagctgttta tcgtataaat aaaatttttc
      181 ttgactatat agttactata tagcttatta tatatagaga gtaataaaag gaggtaaaat
      241 ttatggaaaa tttatttaca agaaaattca ctacttttga atttctaaaa tttgtttctc
      301 ctgcaattat atccatgata tttatatctt tgtacacaat aatagatggt atctttgtat
      361 cgacattagt tggttccaat gctcttgcta gtataaatat tgtactaccc ataattaacc
      421 ttgtttgtgg atttggaata atgatggcaa ctggtggagg agctatcgtt tctatacgta
      481 tgggtgaaaa tagacaggat gaagccaact ctacattttc ttttatagtt ttgttttcat
      541 tgattgttgg gattttattc acagtaatct catatttctt tatcaaagaa atatctatgt
      601 tacttggtgc aacagataag ttattaccat attgtataac ttatggtaaa gttatgattt
      661 tatgtacacc attttatatt ttaaaattta tatttgagta ttttgcaaga actgatggaa
      721 attctaaatt tagtttattt ctatcagtca ttggtggtgt aacaaatata atattggatt
      781 atgtatttat taaatatttt ggaatgggtc ttttaggagc tgcagttgca actgctatag
      841 gtattatttt aacttgtgtt ttaggtatta tttacttctt atctaataaa tctacactaa
      901 aattaagaaa accaaaaact gattttagac ttataagaga tactatgata aatggttctt
      961 ctgaaatggt tacagaatta tctacaggaa ttacaacatt cttatttaat atagtagctt
     1021 taaaattagc aggagaaaat ggacttgctg ctcttactat agtattgtac gctcattttt
     1081 taatgacatc agtctatcta ggattcgctg ctggagtgtc tccattaata agctataatt
     1141 ttggtgctga aaacagtgat aaattaaaag aaacatttaa acattctcta aaatttatat
     1201 ttgtttcttc tcttttagtg tttattattg ctttagtatt tgcaccattt attgttaggg
     1261 tctttgtaaa tccagataat acagtattta aactagcctt acaaggatta aaaatatttg
     1321 catttgcttt tttatttgtt ggtataaata tatttgcatc aggatttttt acagcatttc
     1381 acaatggaaa aatttcagct attatatctt ttagtcgtgc ctttgttttt ataatcatag
     1441 gaatcataat tcttcctcct atgttaaacc tgactggatt atggcttaca gttccatttg
     1501 ctgaggttct aaccctattt atatctattc tatttataaa aaaatataaa ggtagatata
     1561 agtattaaaa attaatattt aaaatcaaca tttactaaaa ctaataaacc tgctttaaaa
     1621 tacaaattta taaatttgat ttttaagcag gttttatttt agttataata agtcatttgc
     1681 atactcttta attggtgctt ttctaagtaa taaaattata aatattatgc aaataactat
     1741 aattcctact tttgttccaa ataaaataaa aggattctga aatgaataaa taattttttc
     1801 taaatacaat agcattttat gcatattttt agttgcaaaa ttaaccatat aaattactat
     1861 taatatcggt aatcctatca atatagtaat actagtaact tttctaactc tatatgtaaa
     1921 agctccaagt aatactccta taaagcctat acatatttca tttatacata tttgtacaaa
     1981 ataatttaaa taagttagcg gtgtacttga tgttgaagga tctatcagtg ctgatataga
     2041 tgtccaaatc atatctctct ttagtaaaac tgatagttct atactgtaac ctgtcataac
     2101 ttctataaat agctttgata aaaaaatcaa aactatacct aaaatagaaa tcacaaagct
     2161 taaaataatt aaatctatga caatagcttt tatacaactt tttctatctg ctcttatact
     2221 taaagcccct gaaaagtctt tatttgctat atttactcca tacacaaatg gcagtatagt
     2281 tatataggtt aaaataggat tcacagttgt agataaatta gttttagatg cctctcccaa
     2341 aattttgagg ataaacactg gcaaaatatt cataacaatt cccacaatca taaatataat
     2401 tatatacaat ttagtatttt tattaaaaaa ttttatatat ggctttaatt cattcaatta
     2461 caattcctcc ttcttagtca tgttaacaaa catatcttgt agccctatgt atcctacatc
     2521 aatagttgaa tttttaagta ttttttcatc attttcacta aaatcaccat agtaaaaata
     2581 agcaactgta tttccaaatg cctttttagg tttcatattt tttatacatt ctaatttttc
     2641 taactcttct ttatttccag taatataatg agctttttgt tttatgatat caatttcttc
     2701 atctataata attttcccct catgcaatat tattacatgc tccagtaaat catctatttc
     2761 atcaatcaaa tgagttgata ttattattgt ccttggattt tttatataac tatctaaaat
     2821 tatgttataa aactcttgtc tatttactgc atccaaacca attgttggct catcaaatat
     2881 ggttatagct gaattagaac aaatacctat tatatttgaa agtatagttt tcatacctct
     2941 tgacagttgt ttatactttt ttttagtatt taaatcaaaa tatttgcata atttttcttc
     3001 taagccttta tcataacttt tataaaaact agaagaaaat tcaaatatct gacctacttt
     3061 aaaatctgga ctataaaatt ctttttccct gacaatacaa acttcttcca aaacttttat
     3121 atcttctttt gggttctttc ctaatatttc tatctcacct tcattttgaa ttaactgatt
     3181 taccattata tttaataatg ttgtttttcc tgctccattc ttacctaata aaccatatat
     3241 cttatttttt tcaaagttta gagacatatt attaaaaata ttttgcttct taaaactgtg
     3301 agataaattt tctaccttaa tagaactact catatattaa tcctccttga aattaattat
     3361 aatatcaacc aattcttctc tatttatttc taatttgtta gcttcttgaa gtagattttt
     3421 tacaaaatta tgtttaaaat tttcttttct agcttctttt ataatagttt ttgcaccatc
     3481 tgatacaaac atccctatcc ccctcttctt atataaaata tttttatcaa ccaatatatt
     3541 tatacctttt aatactgtag ctggattaat tttataaagc tttgaaagct ctgttgttga
     3601 tggaacctgt tcttcttctt ttattccatt tgatagtatc tcatcttcaa ttgctcgtgc
     3661 tatctgaata aatataggtt cttcattatt taatactaat ttcataggtt cctcctttct
     3721 ccattagtta gttactcaac taattaacta tataacttat aatatcttga aaaatatttt
     3781 ctgtcaatac taaataaaaa acagagggaa aattccctct gttttttgtt ctttatattt
     3841 ataattaaat tttagttttt gtaatcctat ccataagtta aaaaccttaa taatctgttg
     3901 tattttcatt ttttaatatt ttacatattg agctagttcc taacctttct gctcctgcct
     3961 ctataaattt ttctgcatca gaaattgatt taactccccc tgcagctttt atttttacat
     4021 tttttccaac atgctctttc ataagcttta tatcttctaa agtagctcca cctgttccca
     4081 ttccagtaga tgtttttata aaatctgaac ctgacattgt tactatttca cacattttta
     4141 ctttttcatc ttcatctaaa taacatgtct ctataatgac ttttaatact ttatctccta
     4201 tagctttttt tatttctttt atttcttttc ctatattatc ataatcttta ttttttatat
     4261 ctgaaatgtt tacaaccata tcaacttcat ctgctccatt ttcaatagca tcttttgctt
     4321 caaatacttt tgtagcagta gtttgatatc ccaaagggaa ccctatcact gtacatatct
     4381 ttattttacc ttttagatat tctgatgctc ttttcacata tgatggtggt atgcaaactg
     4441 atgctacact catatctaca gcttcatcac ataaaatttt aatatcttcc catgtagttg
     4501 tagcttttaa aatagtatgg tctactgttt ttaatatatg tttcataaaa tcctcctaag
     4561 tttataatta atataattag attacttttt taaatgcaat tttctctttg caatgtttag
     4621 tatcatatca acctcttcaa ctttaaaaat agtcattagt agtgtataaa ctccacctcc
     4681 aactataact gaaatagcta gtgatacaaa ctcattaaat gttcctactc ccaaaattcc
     4741 aaatataaat ttatacgtaa agtatgataa aacacccata atcaaagatg caattaccga
     4801 tttcaaagtt gcttttatta ttttatcttg tccaaaatat ccaacttttc tctttaggtt
     4861 caagaagagt aacaatatac atgcaattga tgatgaacta gttgcaagtg caaggccacc
     4921 atgagccatt ggttttatta aaactaagtc cagtactata tttaccccta cagatataat
     4981 cccatttacc ataggtgttt ttgtgtcttg taatgaataa aaaacctttc ccaaaatatc
     5041 tcttaaacca aatgcagtca taccaactgc ataaaaaatt aaggcagttg ctgtcatttg
     5101 ggttgcccta gcatcaaaag cacctctctc aaaaatgatt ctaacaacag gagttgcaaa
     5161 aaaaatagaa gcaactgaaa taggaatcat agaaattatt atcatattta tactagattt
     5221 aactgaacta gtaaacttct tttgattatt ttctgcactt aacttagata acataggata
     5281 cataacagat actattgaag ctgtaaatgt ccctgtaaca aaaccattta atctatccgc
     5341 ataagtaagt gctggaatgc ttcctttaac taatgttgag gctaaagttg tatctaccaa
     5401 tgagttcact tgattaacag caacacctat aaatactggt gaaagtaatg ctagtaattt
     5461 aataaggcta tcatctttaa agtttaagta gtataagtat ttgtagttcg tttttttaac
     5521 aaaaaacatg taaaaaagca gttgaaccac catagcaacc acagcaccaa ttggaagtat
     5581 gtatggtcca aatacggtac ttaacattat tgaaataatt attactatat tatagggtat
     5641 acttccaaat ccaactacaa taaagttatc tttaatttgt aaaaatgctg acataacact
     5701 cgtaatacct atgaatatta ttcctgttat caatatttta gtaaatttta cggtaagtaa
     5761 aaacctctct ccttcaaatc ctatagcaaa tatacttaca agttgctttg agaaaataac
     5821 tcctaatata gctacaacta tgcatatacc aactattata tttaaaacat tgtttaaaaa
     5881 ctttagtgct tgcttttcac cttgtttact acttatatct tgatacattg gaataaaagt
     5941 agtaactata gcagtaccta ttgctgcaaa tataatattt ggtatattca tagctgttaa
     6001 ataactttct gtatataagc ctgtgccata aattgatgat aatactaatt ctctacccat
     6061 tccaagaatc ttagagatta tagtaactat catcaaataa aaagttgctt tagctacctt
     6121 gctcatttta tcactccact tctaacatac ctctttttaa tcaagtaaaa tttggctcat
     6181 tagatataat tctatcaact aatttgcctt gcttagctct atcaaaatat aattaacacc
     6241 tatttcttaa tatatcacaa actacattat tcttcatatt tttttatttc tccagtaagt
     6301 tttacatctt tcccatataa attgtatagc ttcaattata ttcaaagaaa tggagttgct
     6361 acttgataac tttaaaagta aaaaacagat tttattatct ttttaaacta tattctaaca
     6421 tattttgtcc atattgagaa attttactaa actattatgt attttattaa ataaataaaa
     6481 taacagcaca cctataaaat acaagtgtgc tgttcataaa cagattttat aaaaaaataa
     6541 ttcctaagta gagattaaca tgctttctca tactctcata tataatgtgt ttaaaaatgt
     6601 taataaaaat gctttaattg ttttaactta cttggatgtc ttaattttct tattgcttta
     6661 gcttctatct gtcttattcg ttctctggta actccaaata cctttccaat ctgttctagt
     6721 gttttagggt catcatcatc aattccaaat ctcattctta aaacttgctg ttcttgctct
     6781 ccaagacctg taagtagttc ttctatcttt tcttttaaat tatgctgttc aacttcgtgt
     6841 attacaacat ctttaaaatc agcatctgat ggtataaatt caactaaact actatcctca
     6901 tcttctttta atggagtatc caaagataca acatttttat ctcttcttaa taattccaac
     6961 actttgtcaa cttctaagcc acatgcatct gcaatctcat ctatagttgg ctctcttaat
     7021 aatacattta aaagttcttg tttctttttc tttacaaaat ttattctctg atgaagatgt
     7081 ataggtatcc taatagtatt ttcgcaatcg tcgatatatc ttgttatact ttgttttatc
     7141 caccaagttg cataagtgct aaatttatat ccttttttat aatcgtactt ttctacagct
     7201 tttataagtc ctatatttcc tgcttgaatt aaatccaaca tatcaatact atctctttta
     7261 tatctctttg ctatgcttac taccagccta taatttgaat ttataaattt ttcttttgct
     7321 actgaagatg agttgattat ttccttagcc agctctacct cttctcctgc tgacaacatt
     7381 ttgtattcct ctatttcctt taaatacatt ttcatcgcat ttgatacatt tgatgaaccc
     7441 acacaaatat aattttcata attattttct tttttagtat ccataatcaa accccttcta
     7501 ttcatagaat ttgctaaaac aaaaataaat aatttaaacc tacataatct acaattacat
     7561 aatatcccat tctttaaatg aattgttttg tatattatgt atacaaattt ataaaaacta
     7621 tacctataat attattctac ttctaacagc aatatcctcc ttatatatat aatttatttt
     7681 attatatgtt gttatattct tagttttttg aatgatatat gtctaaaaaa agactgttta
     7741 ctaaacagtc tttttttata tatatgctca atatttaatt ttcatgatga tgattacatc
     7801 cacatccaca attttcatca tggtgatgat gttcttctaa atcttttacc tgttgttcca
     7861 tttcatgaaa ctcttttacc attatatcat aagttggaag agctgcctca gtatcgtatt
     7921 cttcccctct ccaatcagcg tacatcatag catctccatt agttaaaacc aatctacctg
     7981 acatagttat attatccact tcttctaaaa attctatttg tttttgtaaa acttctcctt
     8041 ctaactccac atcttcatct aaacattgca ctatcattac aggtgcataa taatcttctt
     8101 catctttaac atttacaaca atatctaaaa ttccttctgc accttcttca aattctgcca
     8161 actcaacatt tgtatcaatc atatcttgag gtactaataa atcttctatt aaatatttta
     8221 ttactctatc ttttactaat tcttttgaca tttatttttc tccttccaat tctataacat
     8281 tatcataatc atttctatat tatagtatac tacattaatt gttttttcaa tctcttacaa
     8341 aaattatttt tttattatct tccttatatt ccctttttta ttacaaagtt gtaatcgcat
     8401 tgatttaaaa aatagtttat atttagtata atagaaatgt agcacatatt tttattcaat
     8461 atattaataa aaaaatagat tgaaaatgta atgtatgtta aatctaggag gaatattatg
     8521 aagtttaaaa aacaaattag ttctattgtt gtgtctacta tgcttgtact tgggagtatg
     8581 aatctttctt atgcagacgg tacaaatgta gttacacttg gagctaactt atcagagtct
     8641 caaaagcagc aaatgcttaa ttactttgga gtaaaaaaag accaagtctt agttttagat
     8701 gtaacaaatg cagaagaaag acagtattta caaggtgttg caacagaggg tcaacttgga
     8761 aaagttacta tctcttgctc atacgttgaa cccactaaaa aaggaaatgg aattaatgtt
     8821 aaaacagcaa acttaacttg ggtaactagt tcaatgattg cttcaacact tacaacagct
     8881 ggtcttgaag atgcaaatgt tattgctgct gctccatatc ctgtaagtgg tactggagca
     8941 ttgacaggaa tcatgaaagc atttgaggat gcttcaggaa agaaacttga tgaaactaag
     9001 aaagaaattg cttctgaaga attggtagtt acgggtgact taggagatga tatagggcaa
     9061 gaaaaagcaa caggtgtaat gaatgatata aaaacagaaa ttgtaaagaa tggtacaaaa
     9121 gataccaatc aaatagcaaa tacaataaat aatgttgtaa ataattacaa tattactatt
     9181 acaccagaac aaaaagaaca aattaaaggt gtaatgaaaa aaatagctga ccaaaattac
     9241 gattataata gcatgaaaaa tacactagat aatgtatcag ataatgtaaa tgaacaatta
     9301 aagaaattag gtgaaagtgt taaaggttct ggcatactag atactattgg tggatggttt
     9361 agtggaatag gagactggtt ctctggttta ttctcaggcg gagataaaaa agatttggga
     9421 atcttaaata acactaatga tgagtctctt ggctctgatg ctgtaataaa ttctacagaa
     9481 ggtgttcaaa taaatccaca aaataatact aattctaaca atgatagtga tacaaatagc
     9541 tcaaaaaatg atacttcaag taataatgaa aataacgata gttctcaaac aaatgaaaac
     9601 aacacaacaa ataattcaga aaatcaatca aataataatg actcagttgg tttctttgaa
     9661 aaaataaaaa attggctttc aggcttattt tagaaattaa acaaataaaa agaattgcct
     9721 aaaatggaaa ttttctataa aaggcaattc tctttattta caataaattt ttattactca
     9781 ctcatttttt tagatttttc tatacatgca catattgctt ttatcacaga agctctaaaa
     9841 ccttcttctt caagtatttt aactgcttct atagtagttc caccaggtga acataccata
     9901 tcttttaatt caccaggatg ttttcctgtc tcaagtacca tttttgcaga acccataaca
     9961 gcctgtgatg caaacttata tgcttgtggt cttggcattc ctgcaattac tgctgcatct
    10021 gctatagctt ctataaataa aaatacatat gctggtgcag aaccacttgc accaattaca
    10081 gcttctataa gatactctgg tactacctca gtatttccaa aagaattaaa tacttctgta
    10141 actgcttcta agtcttcatt gcttatattt ttatttatag aaagagaaga catggcttca
    10201 tttacaagag ctggagtatt tggcatagtt ctaactattt ttttatcttc tcctataata
    10261 gactctatat cctttataga ttttcctgct gctattgtaa ctataatttt attgtcatct
    10321 ataaggtctt ttatctcttc caaaatatca tcatatatat ctggtttaac agccacaatt
    10381 actatatcag aggttctagt tacttccttt gagtcttttg tagtatttat tccaaaattt
    10441 gcatctattc tatcaagagt ttcttgacta tatgctgagg ctataaccat attaggttca
    10501 actaattttg aatttactat tccccctatc atagcgcttg ccatatttcc tgcacctatg
    10561 aatccaattt ttttcacttt agtacctcct tattaactaa tagtaatcgt taaaatttaa
    10621 gcttctttaa ctgcatcaga cttttcaaat ggatttggta tacttactac caaaattttc
    10681 actaccttat tagtatagtt gacccaatta tgattgttct tttcactatt atagtgtatt
    10741 gtgtctcctg gatacatttc tatttgctca tctcccaaaa acactgtaag agtaccctct
    10801 aatacataca caaactcctc accttcatgt acataacaac aaatttcctc actgctctta
    10861 cttggaagaa tttcaattaa tctcggtagc attgtttttt cctttaaact agatgataaa
    10921 tgataatgta taaatgttga gttttctaca tcaaatactt ctttttcata acttcttaat
    10981 acagctcttt tatgctcttt tggcttcata aaaaagtaag ttaattctac atctagtaca
    11041 cttgctattt ttcctaagga atctgttgcc acacttgtta atcctctttc aagttgtgat
    11101 aaaaaaccaa ttgacaagtt tgttttttca ctcatatcct tcaacgtcat ttgcttttga
    11161 gttctgagtt gctttatttt tgctcctatg tccttattca aaaccatcac ccctaaataa
    11221 ttttaatttt ttcataatat aaatacaaaa atataaatat aggtactgaa cctatcttta
    11281 tttcacttaa aaaaattttt ataagtatta ctataattta tcaatcttgt tttgtctaca
    11341 tttttacgat tatttacgat ttatttttca catatgtata tttaatttcg ccaactaaag
    11401 atacattttt tataagcatt tactctttat ataataaaac ttaagttgtt catgcagtct
    11461 ttttctggtt taattattaa aatttcaaat tatattatta tttctacaca ttattacatc
    11521 ctttagtaca attataacat aattttacaa tttaatgata tctattcaat gtcttttttt
    11581 actctacaac atattttatt ttagagttta acactatata tattttttat atacagcctt
    11641 gtcatttttt agattgtcaa gaattattga agtattttat gtagatttaa attttatttt
    11701 ctaatttccc atttaacaag aattgcttat gataaaaa
//
LOCUS       DAEPXF010000120        11687 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain HB27
            SAMN13376389-rid12479423.denovo.126, whole genome shotgun sequence.
ACCESSION   DAEPXF010000120 DAEPXF010000000
VERSION     DAEPXF010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13376389
            Sequence Read Archive: SRR10518432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 11687)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11687)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001069624.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001069624.1
            Genome Coverage       :: 315x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/15/2021 01:54:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,165
            CDSs (total)                      :: 4,076
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 89
            rRNAs                             :: 10, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 10 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 19 of 69
            Pseudo Genes (incomplete)         :: 44 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 10 of 69
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11687
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13376389-rid12479423.denovo.126"
                     /strain="HB27"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="China: Hebei"
                     /collection_date="2017"
     gene            complement(<1..1000)
                     /locus_tag="KRL67_002699"
     CDS             complement(<1..1000)
                     /locus_tag="KRL67_002699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBH1523565.1"
                     /translation="MTLFRIAMQNVKNSFFNYLMYFISIVFSVFVFFSFKSIEYNEAL
                     SSLGEKTRMSINTSSIVIVVFVFLFIYYSNSFFFNRRRREVGTYSLLGMRKNQIGKIF
                     LYETFLMGIVAILIGIFLGLLFSKLMTMMLLKLMNEMIVVNMNLSIKALVQTLAVFLI
                     IFIVIGIRNTIVIRNKRIIELFNKSPEKYRFKKFIKLKCALGVLLIAISYLMSISYFI
                     AENIMLSVFILVTIIPGTFLFFSSAMYMIIDAVKKRKSFYYKGQNLIAFSEFGFKLKS
                     NSSVLAVIAILIATSVTMLGFTISLYYDIDRNISENYKYSYTINLGNSYVNNEIDKL"
     gene            complement(987..1754)
                     /locus_tag="KRL67_002700"
     CDS             complement(987..1754)
                     /locus_tag="KRL67_002700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003722224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBH1523566.1"
                     /translation="MEEILSVENIKKEYGRKGSKHEALRGITFKVYKGEFVGIMGSSG
                     AGKSTLLNIISTIDLPSSGDIYINGKNTIKMKQNELADFRRDNLGFVFQDSNLLDTLT
                     IKENIMLPLSLKNERVSVIENRIKEISKELNIESILDKYPGEVSGGQKQRGAVCRAIA
                     TKPSLVLADEPTGALDSKSARDLLNCLLKLNKDSNKTILMVTHDAISASFCNRILFIK
                     DGIIFTEIVKGESSREFYNKIVNTVSLIGGVNKNDFI"
     gene            2042..2716
                     /locus_tag="KRL67_002701"
     CDS             2042..2716
                     /locus_tag="KRL67_002701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBH1523567.1"
                     /translation="MFKIMVVEDDVSLKNIIAKCLTKWGHDVHQIENLENIIEEFKNY
                     NPELVLLDINLPFYDGFHWCNEIRKISKVPIIFISSRNSNLDVIMGVNLGADDYIQKP
                     FSVDVLVAKVNALLRRTYNFVDNNSNQIIHNGVTLDLSTATINYEDNTIELTKNEIKI
                     LHELMKYKGQIVSRNKLMKKLWDNDWFVDDNTLTVNVNRIRSKLNEIGLEDFIETKRG
                     LGYIIS"
     gene            2740..3744
                     /locus_tag="KRL67_002702"
     CDS             2740..3744
                     /locus_tag="KRL67_002702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HBH1523568.1"
                     /translation="MSFKDFLKDKLGFLVYNFTFLIFTISVITFSPVDVFLTDTILYI
                     LVVNVVFLFLYLFISYIKKNKFLDKIKNDTFKINMNDIELARCKNEEKIYLNIIKNHQ
                     YQCNNIINNNIKKFEENKEILNMWVHDVKIPIAIIKLILEQNENLIDEKVSDDIDSEI
                     FRIENSVEKILYLSRLEDFHKDFLIQEVNIEKIIRNIIKKYSKYFISKKIILDLNNLN
                     FKVLSDEKWLYFIFEQLLSNSLKYSSLGDNISIYCKRTKNYLQLRVEDTGCGIKKEDL
                     NRIFNKGFTGNNGRNNSKSTGLGLYLVKELCDKLGHKIDVDSEYKQYTKFTITFKCDL
                     "
     gene            3856..5085
                     /locus_tag="KRL67_002703"
     CDS             3856..5085
                     /locus_tag="KRL67_002703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HBH1523569.1"
                     /translation="MSFLKKFKNKRFRNNSFKSNNFRNSSFGSSGFKSKNKGFKNNPF
                     KNKRFKIYAGIAVIILLILGVLAYVDSKNTELQANENFIETYTIPENEKIFINGMVVP
                     KQTKDFNISGDYELSDVNVTNGQKVNQGDLLFTAKNPTIIAEIDSLKSQLSQYKKQKI
                     SLSDIAENRDAIASINAQITALNSQIASLDKKAYDRVTAPFDGTVYLNDQTGNPDQPV
                     SFMTIQGLEFYMKGQASEQDLPKMKIDQMVNILVFSTDQKLTGRISFISDKPSTPNTE
                     MGAQQNTLSYYDINIAFDNQEGLVNGFHLQASLEVANSSFKIPASCVLKDNKHSYVFK
                     DLDGILKKQIVDVASQNDDFAVVRGGLEQGDIIIKHPTKEMKEGDPVQGDGVTAGSNN
                     GDTTPNKKVEEVTMDVN"
     gene            5093..5767
                     /locus_tag="KRL67_002704"
     CDS             5093..5767
                     /locus_tag="KRL67_002704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBH1523570.1"
                     /translation="MLIKLENIQKYYKVGKDELHVLKSLNLEIESGEFVMIMGKSGSG
                     KTTLLNILGFLDVFDEGRYIFDGTDVTNLSENERSVFRNINIGFVFQQFNLIETLNVY
                     QNVELPLIYNKVLKKSNREEIVKDKLSSVGLLDKLKQKPLQLSGGQQQRVAIARCLAN
                     DPQIIFADEPTGALDSETSREIMELLTRLNKQGKTIIMVTHDQDLTKYATKVIRLKDG
                     VFTSEV"
     gene            5770..7002
                     /locus_tag="KRL67_002705"
     CDS             5770..7002
                     /locus_tag="KRL67_002705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBH1523571.1"
                     /translation="MSLLKNSLANLKGHKLRVFVALLWIIIGITSVILVSSIGNGFQK
                     EIKKSVNNVNPNKTTISFESADNTGLTDDMSIFLKPFNAKDLEELSFVEGVERIAPSR
                     DGFNLDSVYSSQASFDKKTTYVDVGPVKKDSKINLICGRDFSLDDEKRKVILLTLQST
                     SEIFENPEDALGHGININGTIFEIIGVLDDSQQNQAGGFFGGYQDMQFTTSLVPKKAF
                     DTLMSQNSYSNEIYQLDLVSSKGYNVNEVANNVIAKLYEMHPGINGSYTTPDPTEQTA
                     YLESINSNVNKYVSIITVVAMFVGGIGVMNIMYVSVMERQREIGIRRAIGAKPRSILF
                     QFLVEAVFITVCGGILGTIVGFAATNYVSKYIGFEAIPSLNSLFYAIVATILTGVVFG
                     LIPAFKASKLDPIKAIYK"
     gene            complement(7288..8553)
                     /locus_tag="KRL67_002706"
     CDS             complement(7288..8553)
                     /locus_tag="KRL67_002706"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HBH1523572.1"
                     /translation="MILIKNGRVIDPKSQRDENIDLIIKENKIYKIGKFDESDEYEKI
                     IDASGNVVAPGLVDVHVHFRDPGFTYKEDIESGAKSAARGGFTTVICMANTNPIVDNE
                     DTFNYVKEKSKNACINVLQAAAITKGFEGKELVDMEALKKAGVPGFTDDGLPLMDSNL
                     IMDAMIKAKELDVPLSFHEEDPSLVGNPGVNAGKVASEIGLKGASSVAEDVMVARDCM
                     LALKTGAKVDIQHISSGVSVDMVRFAKYLGANVVAEASPHHFTLTEEDVLEFGTNAKM
                     NPPLRSKWDRDKIIEGLNDGTIEIIATDHAPHSKEEKDREFIKAPSGIIGLETSLALG
                     ITNLVHKNHLSMMQLIEKMSTNPAKLYNLNIGFIEEGAVADIVIFNPEEEWTVESFVS
                     KADNSPFKGKSLYGKVNYTICNGEIVYNA"
     gene            complement(8606..9754)
                     /locus_tag="KRL67_002707"
     CDS             complement(8606..9754)
                     /locus_tag="KRL67_002707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HBH1523573.1"
                     /translation="MKSVSVRSCKTYNYEEVKLSIEKNLEDIGGIDKFVKKGSKVLIK
                     PNLLMKKKPEEATTTHPMVVKVVCEKLLELNCDITIADSPGGPYNQSSLKSIYKASGI
                     EEVANELSIKLNYDVSDVKIHSEKAHVLKYMDIIKPIVDSDYIINLCKLKTHIMATFT
                     GGTKNLYGCIAGLKKAEIHYRFPTEELFCENVLLDICDYIKPTLTIMDGIVGMEGDGP
                     SAGTTREIGVLLASQNPYAIDVVACKIISLLPNRVATIRGAIKRGYIQEDFSDIEILG
                     EDIKDLIVKNYKVPNVSKDLRLFSVKLPKFLNEPISKLITPKPVVRFQDCIKCGKCKE
                     ACPASTIEIGTKGAIIDLSKCIRCYCCHELCPKKAIDVKRNFVFKYVK"
     gene            complement(9845..10390)
                     /locus_tag="KRL67_002708"
     CDS             complement(9845..10390)
                     /locus_tag="KRL67_002708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH peroxidase"
                     /protein_id="HBH1523574.1"
                     /translation="MKKFVCTVCGYIHEGDAAPAQCPVCKVGADKFEEMKGEMVWADE
                     HRIGVAQGVDAEIIEGLRANFTGECTEVGMYLAMSRQADREGYPEVAEAYKRIAFEEA
                     EHAAKFAELLGEVVVADTKENLRVRVDAEYGATDGKLKLAKRAKELGLDAIHDTVHEM
                     CKDEARHGKAFLGLLNRHFGK"
     gene            complement(10613..11557)
                     /locus_tag="KRL67_002709"
     CDS             complement(10613..11557)
                     /locus_tag="KRL67_002709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HBH1523575.1"
                     /translation="MQKNKKEGALLKKYFGEIGLIFIAIIWGSGFVATQFALDGGLTP
                     LQIITLRFFLAAIIMNLLFFKQIRANMSKKLLKAGGVLGIFLFLAFTVQTIGLMYTTP
                     SKNAFITAANVVIVPFIGFILYRRKLDKIGIISSLVALIGIGILSLEADFSINFGDFL
                     TLICSFGFAFHIFFTSEFAKDNNPMALTAIQFTVAFLMSVVVQTFAGQLKMEAELSGY
                     MGTMYLAVFSTTIGFLFQTICQKRVDGTRTAIILSTEAVFGTIFSIIILKELITAKLV
                     IGSILIFVAIITAETKLSFLKSKKVKLKDSEESSLESI"
BASE COUNT         4181 a         1817 c         1464 g         4225 t
ORIGIN      
        1 ataatttgtc aatttcgtta tttacataag aatttcctaa atttatagta taactgtatt
       61 tataattttc acttatattc ctatctatat catagtaaag tgatatggta aatcctaaca
      121 tagttacact tgtagctatt aatattgcta ttacagccaa tacactgcta ttgctcttta
      181 atttaaatcc aaattcagaa aatgcaatta aattctgtcc cttataataa aaactttttc
      241 tcttctttac agcatcaata atcatataca tagctgatga aaaaaataaa aaagttccag
      301 gtattattgt aactaatata aaaactgaaa gcataatatt ttcagcaata aaataactaa
      361 ttgacattaa ataagaaata gctattaata aaacaccaag tgcacatttt aactttataa
      421 attttttaaa cctatacttt tctggagatt tattaaataa ctctataatt ctcttatttc
      481 tgataactat agtatttctt attccaataa ctataaatat tattaaaaat accgctagtg
      541 tctgaactaa ggcctttata cttaagttca tatttactac tatcatttca ttcattaatt
      601 ttaaaagcat catagtcata agctttgaaa ataacagtcc taaaaatata cctattagta
      661 ttgcaactat acccatcaaa aaagtttcat ataaaaatat ttttcctatc tgatttttcc
      721 tcattccaag taaactataa gttcctactt ctctcctacg tctattaaaa aaaaatgagt
      781 ttgaataata tataaataaa aatacaaata ctactattac aattgaactt gtattaatac
      841 tcatcctagt tttctctcca agtgaactca atgcttcatt atactctata gacttaaaac
      901 taaaaaatac aaatacacta aatacaattg aaataaaata cattaaataa ttaaaaaaac
      961 tattctttac attttgcatt gcaattctaa ataaagtcat ttttattaac tcctccaatt
     1021 aaggaaactg tatttactat tttattatag aactctctgc tactttctcc ctttacaatt
     1081 tctgtgaata ttattccatc ctttataaag agtattctat tgcaaaaact cgcacttata
     1141 gcatcatgag ttaccatcaa tattgtttta ttgctatctt tgtttaattt taataaacaa
     1201 ttcaacaaat ccctagcaga tttagaatct aatgctcctg taggctcatc tgcaagtact
     1261 aatgatggtt ttgttgctat ggctctacat acagctcctc tttgcttttg tccaccagaa
     1321 acttcgccag ggtatttatc taatatagat tctatattta attcttttga aatctctttt
     1381 attctatttt ctattactga tactctttca ttttttaaag aaagaggcaa cattatattt
     1441 tcttttattg taagtgtatc caaaagatta gaatcttgaa atacaaagcc tagattatca
     1501 cgtctaaaat ctgccaattc attttgcttc attttaatag tattctttcc atttatataa
     1561 atatctcctg atgaaggtaa atctattgta gaaataatat ttaaaagtgt tgatttacca
     1621 gcaccagaag accccataat accaacaaat tctcctttat agaccttgaa agttattcct
     1681 cttagagctt catgttttga accttttctt ccatactcct tttttatatt ttcaacagat
     1741 aatatttctt ccatagttaa taatcacctt tcatcataat tagttattta tcaattctca
     1801 caaatatatt atatatatta aaatggcttt taaacattta attatattaa tttaaggtta
     1861 cattttcgta agtattaatt atatttcttc aaatttattg caattatgtt taaaatataa
     1921 tttatcaagt aaaatatagt tatattaata taattatttt aggtaataag gtagaaatat
     1981 tttaatttat caagtagaat atagttatgt taatataact attttgaatt aggaggtaag
     2041 aatgtttaaa ataatggtag ttgaagatga cgtctcattg aaaaatatca tagcaaagtg
     2101 tcttactaaa tggggacatg atgttcatca aatagaaaat cttgaaaata taatagaaga
     2161 gttcaaaaac tataacccag aattagtttt attggacata aaccttccat tttatgatgg
     2221 ttttcattgg tgtaatgaaa taagaaaaat ttcaaaagtt cctataattt ttatttcttc
     2281 aagaaattct aatctggatg taataatggg tgtaaattta ggtgcagatg attatataca
     2341 aaaacctttt tctgtagatg tcttagtagc taaagttaat gcccttttaa gaagaactta
     2401 taattttgtt gataataact caaatcagat tattcacaat ggtgttactt tagatttatc
     2461 aactgcaaca ataaactatg aagataatac tattgagcta acaaaaaatg aaatcaaaat
     2521 tctccatgag cttatgaaat ataaaggtca gatagtaagt aggaataaac tcatgaaaaa
     2581 attatgggat aatgattggt ttgtagatga taatacatta actgtcaatg taaatagaat
     2641 acgctcaaaa ttaaatgaaa ttggtttaga agattttata gaaacaaaaa gaggattggg
     2701 ttatataatc agttaaaaca attctaagta ggtgaattta tgagctttaa agattttctc
     2761 aaagataaac taggtttttt agtatacaat tttactttct taatttttac aatatcagtt
     2821 attacatttt ctcctgtaga tgtttttctg actgatacta ttttatatat attagttgta
     2881 aatgttgtgt ttttattttt atatctattt attagctaca tcaagaagaa caaattttta
     2941 gataaaataa aaaatgatac attcaaaatt aatatgaatg atatagaatt agctagatgt
     3001 aaaaatgaag aaaaaatata cttgaacatt attaaaaatc atcaatatca atgtaataac
     3061 ataataaata ataatattaa gaaatttgaa gaaaacaaag agattttaaa tatgtgggta
     3121 cacgatgtaa agatacctat agctataata aaactcattc ttgaacaaaa tgaaaattta
     3181 attgatgaaa aagtttcaga tgacatagat agtgaaatat ttagaattga aaactccgtt
     3241 gaaaaaattt tatatttatc aagactcgaa gattttcata aggatttttt aattcaagag
     3301 gtaaatattg aaaaaatcat tagaaatatt ataaaaaaat actcaaaata ttttatttca
     3361 aaaaaaatta ttttagactt aaataatttg aattttaagg ttttatctga tgaaaaatgg
     3421 ctatacttca tattcgagca attattgagt aattccctaa aatactcctc attaggagat
     3481 aatatatcta tctattgcaa aagaaccaaa aattatttac aattaagagt ggaagatact
     3541 ggttgtggta taaaaaagga agatttaaat cgaatattca ataagggatt tacaggtaat
     3601 aatggaagaa acaattctaa atctacaggt cttggacttt atttagtaaa agaactttgt
     3661 gataagcttg gtcataaaat tgatgtagat tcagagtaca agcaatatac aaaatttact
     3721 attacattca aatgtgattt atagtatctt attattgata gtttattttc attaacttat
     3781 atttaacttc ttatagtaat tctatatgta catcccagta tataattaag tagtttttat
     3841 aaaaaggagg aagacatgtc ttttctaaaa aaatttaaaa ataaaagatt tagaaataac
     3901 agttttaaaa gtaacaactt tagaaatagc agttttggaa gtagtggctt caagtctaaa
     3961 aataaaggtt ttaaaaacaa tccctttaaa aataagagat tcaaaatata tgcaggaata
     4021 gcagtaatca tacttttaat tctaggagtt cttgcttatg ttgattcgaa aaacactgaa
     4081 ttacaagcta atgaaaattt tatcgaaaca tatactattc ctgaaaatga aaaaatattt
     4141 ataaatggga tggtcgttcc taagcaaact aaggacttca atatatctgg agattatgag
     4201 ttaagtgatg ttaatgttac aaatggtcaa aaagtaaatc aaggagacct tctttttact
     4261 gctaaaaatc caactataat agcagaaata gatagtttaa aatcacaact tagtcaatac
     4321 aaaaaacaaa aaatatcact ttctgatatt gctgaaaata gagatgctat tgcttctata
     4381 aatgcacaga taactgcttt aaactctcag atagcttctc tagataaaaa agcgtatgac
     4441 agagttactg ctccttttga tggcacagta tacttgaatg accaaactgg aaacccagac
     4501 caaccagttt catttatgac tattcaaggt ctagagtttt atatgaaagg acaagcaagt
     4561 gaacaagatt tacctaaaat gaagattgac caaatggtaa atatactagt tttttcaact
     4621 gaccaaaagc ttactggtag aataagtttt atatctgata aaccatctac tccaaatact
     4681 gaaatgggag ctcagcagaa tacactatct tattatgata taaacatagc atttgataat
     4741 caagaaggat tagtaaatgg attccatctt caagcatcac ttgaagtagc taattcaagt
     4801 tttaaaatac cagcatcttg tgtacttaaa gacaacaaac actcatatgt atttaaggat
     4861 ttagatggta tattgaaaaa gcaaattgta gatgtagcaa gtcaaaatga tgattttgct
     4921 gtagttagag gcggtcttga acaaggcgat ataataataa aacaccctac taaagaaatg
     4981 aaagaaggag acccagttca aggcgatggc gtaactgctg gttctaataa tggagatact
     5041 actccaaaca aaaaagtaga agaagttact atggatgtga attaatttac atatgttaat
     5101 taaattagaa aatattcaaa aatattacaa agtagggaaa gatgaattac atgttttaaa
     5161 atccttaaat ttagaaattg aatctggaga atttgtcatg ataatgggta aatctggtag
     5221 tggtaaaact actttactta atatcttagg ttttctagat gtatttgatg aaggaagata
     5281 tatatttgat ggaacagatg taactaattt gagtgaaaat gaacgttctg tttttagaaa
     5341 tattaatatt ggatttgttt tccaacaatt taatttaata gaaactctaa atgtatatca
     5401 aaatgtagag ttgcctctaa tttacaataa agtactaaaa aaatctaata gagaagaaat
     5461 tgttaaagat aaattaagtt ctgttgggct tttggacaaa cttaagcaga aaccacttca
     5521 attatctggt ggtcaacaac agcgtgtagc cattgcacgt tgtctagcaa atgaccctca
     5581 gataatcttt gcagatgaac cgactggggc attagatagt gaaactagca gagaaattat
     5641 ggagctttta acaaggctca acaaacaagg taagactata ataatggtta cacatgatca
     5701 agatttaact aagtatgcaa ctaaggttat acgacttaaa gatggtgtgt ttacttcgga
     5761 ggtgtaatca tgagtttatt aaaaaattct ttggccaatt taaaaggtca caagcttcgt
     5821 gtatttgtag ctttactctg gattataata ggaataactt cagtaatcct tgttagttct
     5881 ataggtaatg gatttcaaaa agaaattaag aaatctgtaa acaatgtaaa tcctaataaa
     5941 actactattt cgtttgaatc agccgataac acaggtctta ctgatgatat gagtattttt
     6001 ttaaaaccat ttaatgcaaa agatttagaa gaattatcat ttgttgaagg agtagaaaga
     6061 atagctcctt caagagatgg gtttaacctt gattcagttt actcttcaca agcatctttt
     6121 gataaaaaaa ctacgtatgt tgatgttgga ccagtaaaaa aagattctaa aataaattta
     6181 atttgtggta gagatttttc tttagatgat gaaaaaagaa aagtaatttt acttacatta
     6241 caaagtacaa gtgaaatttt tgaaaatcca gaagatgcct taggtcatgg tatcaacata
     6301 aatggaacta tatttgagat aataggagta ttagatgatt ctcagcaaaa ccaagctgga
     6361 ggattctttg gaggttacca agatatgcaa tttactactt ccctagtacc taaaaaggct
     6421 tttgacactt taatgagtca aaattcttat tcaaatgaaa tttaccagtt agacttagtt
     6481 tcttctaaag gctataatgt aaatgaagtt gcaaacaatg ttatagctaa attatatgag
     6541 atgcaccctg gtatcaatgg ttcttatact acaccagacc caactgaaca gacagcttat
     6601 cttgaaagca ttaattccaa tgtaaacaaa tatgtatcta taataactgt agttgcaatg
     6661 tttgttggtg gtattggtgt aatgaatata atgtatgtat ctgttatgga aagacaaaga
     6721 gaaattggta taagacgtgc tattggagca aaacctaggt ctatattatt tcaattttta
     6781 gttgaagctg tttttataac agtatgtggt ggaattttag gaactatagt tggatttgct
     6841 gctactaact atgtttctaa atatataggt tttgaagcga tacctagctt aaatagctta
     6901 ttttatgcaa ttgtagctac tatattgact ggagttgtat ttggtctaat tccagccttt
     6961 aaagcctcta aactagaccc tataaaggca atttataagt agaaattgta taaagatttt
     7021 cgatttttag attaatttat ataaagcata cttaaatata taacgcacta ttatacatga
     7081 tagattaaga acttaatctt tccaaatata atagtgcgtt ctttcataaa aaggattctc
     7141 ttccttacta tatctaaatt accaatatat catgttctaa ttgttcaaac taatactttc
     7201 aatttgaatt aatcaatctc attctcctaa tatacaatct tttactttca agcattgtac
     7261 tctctgcctt taaaaaataa tcttaaatta ggcgttatat acaatctccc cattacatat
     7321 tgtataatta acttttccat ataaagattt acctttaaat ggtgaattat cagctttaga
     7381 aacaaagctt tcaactgtcc attcttcttc tggattgaat ataacaatgt ctgctacagc
     7441 cccttcttct ataaatccaa tatttaaatt gtacaacttt gcaggatttg tagacatttt
     7501 ttctattaac tgcatcatac taagatgatt tttatgcacc aaatttgtta ttccaagagc
     7561 taaagaagtt tcaagcccaa taattccact tggagctttt ataaactctc tatctttttc
     7621 ttctttacta tgaggtgcat ggtccgttgc tattatttca atagtaccat catttaatcc
     7681 ctctataatc ttatctctat cccatttact tcttagaggt ggattcattt ttgcatttgt
     7741 tccaaactcc aatacatctt cctcagtcaa tgtaaagtga tgtggtgaag cttctgcaac
     7801 tacatttgca cctaagtatt ttgcaaatct aaccatatca acagatacac ctgaacttat
     7861 atgttgtata tcaaccttag caccagtctt taatgccaac atacaatctc tggctaccat
     7921 aacatcttct gccacacttg atgcaccttt taatcctatc tctgaagcta cctttcctgc
     7981 atttacacct ggatttccaa ctaaagaagg gtcttcttca tgaaaactaa gtggtacatc
     8041 caactctttt gccttaatca ttgcatccat aattagattg ctatccatta aaggtaatcc
     8101 atcatcagta aatcctggta caccagcttt tttaagagct tccatatcaa ctaactcttt
     8161 accttcaaac cctttagtta tagctgctgc ttgtaaaaca tttatacatg cattttttga
     8221 cttttcttta acataattaa aagtatcttc attatctaca ataggattag tatttgccat
     8281 acatataaca gttgtaaatc cacctctagc agcagattta gctccacttt ctatatcttc
     8341 tttatatgta aacccagggt ctctaaagtg tacatgtaca tccactaaac ctggcgcaac
     8401 tacattacca ctagcatcta ttattttttc atattcatca ctttcatcaa actttcctat
     8461 tttataaatt ttattttctt ttataattaa gtcaatattc tcatctcttt gacttttagg
     8521 gtctattact ctaccatttt taattaaaat catattattt ctcctttata tttttaattt
     8581 atatagactc atataatatt atacattatt ttacatactt aaatacaaaa ttacgtttga
     8641 catcaatagc ctttttagga caaagttcat ggcaacagta acatcttata catttactta
     8701 agtctataat agcaccctta gtccctatct ctatagtgga tgcaggacaa gcttccttgc
     8761 attttccaca ttttatacaa tcttgaaatc taaccacagg ctttggtgtt ataagctttg
     8821 aaataggttc atttaaaaat tttggtagct ttacggaaaa caatcttaaa tctttactta
     8881 catttggaac cttgtaattt tttacaatta aatcttttat atcttcacct aaaatttcaa
     8941 tatctgaaaa atcttcttgt atatagcctc tcttaatagc acctctaatt gtagcaactc
     9001 tatttggaag taaacttata atcttacatg caacaacatc tatagcatat ggattttgtg
     9061 atgctaataa aactcctatt tctcgagtag taccagctga aggtccatca ccttccatac
     9121 ctactatacc atccataatt gtcaatgttg gttttatata atcacaaata tctaaaagta
     9181 cattttcaca aaacagctct tctgtaggaa atctataatg tatctctgct tttttcaatc
     9241 cagctataca tccatataag ttttttgtac caccagtaaa agttgccatt atatgagtct
     9301 ttaatttaca aagatttatt atgtaatctg aatctactat tggtttaata atatccatat
     9361 attttagaac atgtgctttt tcactatgta tcttaacatc tgaaacatca taatttaact
     9421 taatgctcag ttcatttgct acttcttcaa ttccagatgc cttataaatg ctcttaagag
     9481 aactttgatt atatggacca ccagggctat ctgctattgt aatatcacaa tttaactcta
     9541 aaagtttttc acatacaact tttacaacca taggatgtgt agttgtagct tcttcaggct
     9601 tttttttcat caataaattt ggttttataa gtactttact tcctttttta acaaacttat
     9661 ctataccacc aatatcctct aaattttttt caatagataa ctttacttct tcataattgt
     9721 aagttttaca actcctaaca gataccgact tcatattatc tccatccttc tatcacaaca
     9781 gtttatttgt ataatttaaa aaaggagaaa ccaaagtttc tccttttaat ctatgtttta
     9841 attattattt tccaaaatgt ctgtttaata atcctaagaa tgctttacca tgtctagctt
     9901 cgtctttaca catttcatgt actgtatcat gtatagcatc taatcctaat tctttagctc
     9961 tcttagctaa ttttaatttt ccgtcagttg caccatactc agcatcaact ctaactctta
    10021 agttttcttt tgtatctgca actacaactt ctccaagaag ttctgcaaat ttagcagcat
    10081 gttcagcttc ttcaaaagct attctcttat aagcttcagc tacttctgga taaccttctc
    10141 tatcagcttg tctactcatt gctaaataca ttcctacttc tgtacactca ccagtaaagt
    10201 tggctcttaa tccctcgatt atttctgcat ctacaccttg agctactcct attctatgtt
    10261 catcagccca aaccatttcg cctttcattt cttcaaattt atcagctcca actttacata
    10321 ctggacattg tgctggtgca gcatctcctt catgtatata tccacatact gtacaaacaa
    10381 attttttcat aattaatttc ctcccaattt tatataatta aatttttatt tttaagttta
    10441 ataacttgct acaagaatat atatacccca gactttatag cttaaacact aaaaataaaa
    10501 tttatttcct aattaatttt atcactttga aacaccagta tcaaccaagt tttgatgtca
    10561 aatttctaca taatacataa tttatttgat taattaggaa ataattgtca tattaaatac
    10621 tttctaatga agactcttct gaatccttaa gtttcacttt tttgctcttt aaaaaagata
    10681 atttagtttc tgcagttatg attgcaacaa atatcaagat gctaccaata accaattttg
    10741 ctgtaataag ctcctttaga attattatag aaaatattgt accaaataca gcttctgtgg
    10801 ataatattat agctgttcta gttccatcaa ctcttttttg acatatagtc tgaaataaaa
    10861 atccaattgt agtactaaat acagctaggt acatagttcc catgtaacca gataattcag
    10921 cttccatctt taattgacct gcaaatgttt gaactacaac agacattaaa aacgctactg
    10981 taaattgaat agctgtcaat gccattgggt tgttatcttt tgcaaactca cttgtaaaga
    11041 atatatgaaa tgcaaatccg aatgaacata taagtgttag aaaatctcca aaattaatag
    11101 agaaatcagc ttctaaagaa agtatcccaa ttcctataag agctaccaaa ctacttatta
    11161 ttcctatctt atcaagtttt cttctataaa gtataaatcc tatgaatgga actattacaa
    11221 cattagctgc tgttataaac gcatttttag atggtgtagt gtacataagt ccaatagttt
    11281 gtactgtgaa ggctagaaat aaaaatattc ctaaaacccc tcctgctttt aatagttttt
    11341 tactcatatt ggcacgaatc tgtttaaaaa acagtaaatt cataattata gctgccaaga
    11401 aaaatcttaa agttatgatt tgtagaggtg taagcccacc gtccaatgca aattgggttg
    11461 ctacaaagcc acttccccat ataattgcta taaatattag tcctatttct ccaaagtatt
    11521 ttttcaataa agccccctcc tttttatttt tttgcaaaat tataaacccc ctaaacagca
    11581 agagagtttt tctataagca ttactataaa ttaatatagt aaattttttc agtattttaa
    11641 atctaaattt acatacattt tgagctcaaa tttatatcca ctattta
//
LOCUS       DAEPXF010000121        11622 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain HB27
            SAMN13376389-rid12479423.denovo.127, whole genome shotgun sequence.
ACCESSION   DAEPXF010000121 DAEPXF010000000
VERSION     DAEPXF010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13376389
            Sequence Read Archive: SRR10518432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 11622)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11622)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001069624.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001069624.1
            Genome Coverage       :: 315x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/15/2021 01:54:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,165
            CDSs (total)                      :: 4,076
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 89
            rRNAs                             :: 10, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 10 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 19 of 69
            Pseudo Genes (incomplete)         :: 44 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 10 of 69
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11622
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13376389-rid12479423.denovo.127"
                     /strain="HB27"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="China: Hebei"
                     /collection_date="2017"
     gene            63..692
                     /locus_tag="KRL67_002710"
     CDS             63..692
                     /locus_tag="KRL67_002710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH1523576.1"
                     /translation="MNKYLLINPVAERMYGEKFHLIKANLIKKGYIIAECEPQLEYVK
                     KQYKEYTKKTENTMLDCRCPESISLLKRNNLINNFEVPMIEPILVRTCRVLYDKYIKS
                     KDDMLVVTCPCTQLRDFASEKFKDKGKAVFYTWKEFAEQEGVKSLGKIDESPIPLGYF
                     KDTFEKVLEVSSEDEILSEIQKIRNGSEDKYDIVEMFYCKDGCNNGDGL"
     gene            693..1475
                     /locus_tag="KRL67_002711"
     CDS             693..1475
                     /locus_tag="KRL67_002711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBH1523577.1"
                     /translation="MVNKFNNKFLRLVKTVLIFFIILLLWKITNYLGIWSDYILPSPE
                     KVYSTSLKMISDGSIFINVYASMKRVLIGFAISTAIGVPLGIFFGIYSGVYEYFKSLI
                     NFLRNTPPLALIPMLILWFGIGEESKIIIIVLASFFPIFTSTLKGIKNCDSKLIEVGR
                     VFEFSKLQIIFKIIIPNAILDIAVGLKLALGYSFRAIIGAELVAASSGLGYLISDGKE
                     MSRTDVVIVGIIVIGLLGIITDYIFSIIVKKVSKGKMVEAYE"
     gene            1468..2346
                     /locus_tag="KRL67_002712"
     CDS             1468..2346
                     /locus_tag="KRL67_002712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBH1523578.1"
                     /translation="MNNLVCDSGYIIRNLYKSYEVDNEEHVVLSDISLNIDRKHITVI
                     LGESGCGKTTLLRAIASLEGINSGEIRFINDNKEYRPNVGFVFQESRLMPWLNVSENI
                     AFHNQRKNTILNKVLRKFKHNKRIIGKMINDNFRSLETNDNLNKSNKYNRKSNIDVEK
                     YLKMMNLQKFKNSYPNELSGGMAQRVSIARALSFNPDMLFMDEPFSALDYFTRIDMQN
                     EVIRIHKSTNKGVIFVTHDIDEALKIGKKIVVFTDERKIKEFNIEDGYNRDLTSEYYI
                     KLKKDILMTMKSNKGI"
     gene            2379..3338
                     /locus_tag="KRL67_002713"
     CDS             2379..3338
                     /locus_tag="KRL67_002713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NrtA/SsuA/CpmA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HBH1523579.1"
                     /translation="MKLKKLLIGITILTLATASLAGCAKKNNGEKLSEINLTYVKSPL
                     NVPSIIQKQDDLFGKEFKKDNIKVNFHEITTGPEQTQALAAGEIDFLHALGGTSALIA
                     ASNGVELKILNTYSRSPKGFMILTNNNSIKSAADLVGKKVAGPKGTILHQVLISALDK
                     EGLSMDDVEFVNMGIPEASAALSDGSVDAALIAGPAALKAMKSGSKLVANGEGLVDGI
                     IVTAVSTDFAEKHPEIVERFMKVEKETLEYVNNNFDEAMEKVAKEVDLSLEETKELYA
                     WYDFSLDITDKDISSLEDTQDFLIKNKLQEKKVNIKELIYNAK"
     gene            3652..4344
                     /locus_tag="KRL67_002714"
     CDS             3652..4344
                     /locus_tag="KRL67_002714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011987369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBH1523580.1"
                     /translation="MSYKVYIVEDDISISTLLKDYIEKYGFEVIVEENFDDIMITFES
                     FKPHLVLLDVNLPKFDGFYWCRCIRQQSNLPIIFISARDGNVDQIRALESGADDYITK
                     PFYYDVVMAKIKSHIRRVYGEYSPKIDERIVELDGLKFYPERLELELNGENLIITKKE
                     GILIECLIKRYPKVVSRDYLLEKIWDDIEFVEENTLNVNVSRIRKRFEELGIDNAVQT
                     VRGLGYKLNKNW"
     gene            4354..5358
                     /locus_tag="KRL67_002715"
     CDS             4354..5358
                     /locus_tag="KRL67_002715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBH1523581.1"
                     /translation="MKLFLKDYKGYIFIYYIGIVMTIIYCNLMKFISFGESLYILLFS
                     SFILLCFLIYKYYKNREVYNLFEKGVHSLEESCLYLGSSFLGRNISVILKQLHKLYQS
                     MIQEHKKIHSEHLTFINQWIHQMKTPISVISLQLQDYEGEEVTENIREEIDRLNKGLD
                     MAMHFARADNFQKDFIVEKAKLNEIVSSIVNEEKRLFIKSGMIPKIQIDKFTYVYTDV
                     KWMKFIIGQLITNGVKYSKDYSNYLTIKSEENNKYVILSIIDEGIGIVSKDLKRVFDP
                     FFTGENGRKFGESTGMGLYLVKKVCDDLGHKVSIKSEVGKGTEVSISFNKDVNSLIKV
                     "
     gene            5613..6464
                     /locus_tag="KRL67_002716"
     CDS             5613..6464
                     /locus_tag="KRL67_002716"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HBH1523582.1"
                     /translation="MSLDVIFILKSVIIAIVEGLTEFIPVSSTGHMILVGNLIDFKGQ
                     FAEMFEVVIQLGAILAVVVLYWKKIKDSVIEFFKFIFTGGKEGKIGFRFGMNVIIGCI
                     PFAIIGVLFYDNIKSLFNLQSVIIGFIVGGILLLVVETLFRKKNHSTDNIDKITPIQA
                     LKVGTLQVLSAWPGMSRSASTIMGGWIAGLNSPTAAEFSFFLAVPAMVASSGKDLFEF
                     DYSIMTPTLWIALVVGFIVAFIVSIIVMEKFVNFLKKKPMRVFAIYRIIMGVVLAVLA
                     FTNIISV"
     gene            6566..7312
                     /locus_tag="KRL67_002717"
     CDS             6566..7312
                     /locus_tag="KRL67_002717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001637630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBH1523583.1"
                     /translation="MKILTVNNLSKVHGKKIIFNALNDINFSIEYGEFVGIMGPSGSG
                     KTTLLNMISTIDKPTTGTMELKGKNLLLLKGEELALFRRRELGFVFQDFNLLDTLTIG
                     ENIVLPLTLDKVSVKEQDKRLNEVSEILGIKDLLGKRTFEVSGGQAQRTAIARALINK
                     PSILLADEPTGNLDSKSSKTVMELFQKINKENKVTTMMVTHDPLAASYCSRILFIKDG
                     AIYNEIYKGSSREQFYQEIMDVLTLLGGDN"
     gene            7312..9228
                     /locus_tag="KRL67_002718"
     CDS             7312..9228
                     /locus_tag="KRL67_002718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBH1523584.1"
                     /translation="MNIKQFAINNISRNIKAYLGYLASIIISSSLLFSFIMFIAHPDL
                     DVSQFPDYLKEGYNMSKIIAYLFLFFFVFYSVSVFLKSRYKEFGILYITGISKRQVMK
                     LIFIENMIINIVSSVIGIIIGLIFAKIFLVVMSTFLELSPLKFYIPFNAILSTLIYFM
                     ILAVLTSIFTSFIIKENQVLKLLKGTKTPKPEPKTSLMLAILSICLFIIAYYNAVTST
                     DQYTTYLRLVPVTSLTIVATYLFFSQFSVFFIKKLKLNKRFYRKNTNMLWISNLIYKV
                     KDNARIFFLITITSAVAFTAIGTVYSFWKDVNRQINLIYPNTIYYSTMTLHNDPKKPD
                     SKEKDKERMRFIENNLKKENIDYTRINGEFKTVFPKKSDFNVRIMKESKYLEITEVIG
                     VKPISFEDNESISLLSSSLPGNKKIKENVIVGNRSLKVVKQVEECVMPAYYKNMYVVK
                     DNFYDSIESKFIVDRFTAFEVEDSSKTIEICREFENKFNNESGMQPYLFFSKELMIET
                     GKLMYSTFIFLAIFIGLIFFVTTSSFLYNKFYMDCQVDKKKYEQLNKLGMTYNEIKKA
                     STIEIGIVFLFPYIVAVIHSIFALTALKNSFDIEVASSAVLVMGSLFLVQIVYFLIVR
                     NNYLKEIRLNLVNF"
     gene            9507..9740
                     /locus_tag="KRL67_002719"
     CDS             9507..9740
                     /locus_tag="KRL67_002719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HBH1523585.1"
                     /translation="MMILIQSRGKMKCKELSEELEVSERQIKSYKMYLEQAGIFINST
                     PGIYGGYEIDKCNSISLIKLLDSEVSIFILTKW"
     gene            9936..10364
                     /locus_tag="KRL67_002720"
     CDS             9936..10364
                     /locus_tag="KRL67_002720"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HBH1523586.1"
                     /translation="MIRKLNNKDINKIMEIWEKSTIKAHDFISKEYWQDNYNAVKNEY
                     IPISDTFVYDDGDEIKGFISIIDKSFIGALFIKPKYQNIGIGGKLLDYATKKYKSLSL
                     AVYKDNKKAVVFYNKKGFNIVKEQVNEDSGFKEYIMEYSK"
     gene            complement(10829..11608)
                     /locus_tag="KRL67_002721"
     CDS             complement(10829..11608)
                     /locus_tag="KRL67_002721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH1523587.1"
                     /translation="MKVNENNINNIDEELDELSRKVYLESPDFWISISDKIIDKVFDE
                     MLLFFAVKYNYFNIVCFAIENNYLDLNMPSKNKKYTNIIEHLLDTSKQNEDKKIYNYL
                     LSLNKGYSLDERPILDELNCNISSDTFEKKDIYLPKYLCPSCNSNIFDTGYKVSNDIT
                     YKFSSKEAEPIEMCSEVSSIRCCNCDELLKDITLDKLYNLCKIQNCNNCGSNLTKVGI
                     IAKKKMNFDENTEKFKDVSTSYCCGNCDSEINLSQREHFKI"
BASE COUNT         4578 a         1122 c         1930 g         3992 t
ORIGIN      
        1 ttaagtgctc atttaaacta ttaattgata ataatattca atattaaata ataggaggcg
       61 ttatgaataa atatcttcta ataaatccag tggcagaaag aatgtatgga gaaaagtttc
      121 accttattaa agcaaatttg ataaaaaagg ggtacataat agcagaatgt gaaccacagt
      181 tagaatatgt aaaaaagcag tacaaagaat atacaaaaaa gactgaaaat acaatgttgg
      241 attgtagatg tccagaaagt ataagccttt taaaaagaaa taatttgata aataattttg
      301 aagtacctat gatagaacca atattagtaa gaacttgtag ggttttatac gataaatata
      361 taaaaagtaa agatgatatg ttggtagtaa cctgtccatg tacacaatta agagactttg
      421 caagtgaaaa gtttaaggat aagggtaagg ctgtgtttta tacatggaaa gagtttgctg
      481 aacaagaagg tgtaaaatca cttggaaaaa tagatgaatc accaattcca ttaggatatt
      541 tcaaagatac ttttgaaaaa gttcttgaag tatcaagtga agacgaaata ttaagtgaaa
      601 tacaaaaaat aagaaatggc tctgaagata agtatgacat agtagaaatg ttttattgta
      661 aagatggttg taacaatgga gatggtttgt aaatggtcaa taaatttaat aataagtttt
      721 taaggctagt aaagactgtt ctgatatttt ttataattct tttattatgg aaaattacaa
      781 attatcttgg tatttggagt gattatatat taccatcacc cgaaaaggta tatagtacat
      841 ctttaaagat gataagtgat ggttcaatat ttattaatgt atatgcaagt atgaaaagag
      901 ttttgatagg ttttgcaata agtactgcta taggtgtgcc tttagggata ttctttggaa
      961 tttatagtgg agtatacgaa tactttaaat ctctaataaa ttttttaagg aatacacctc
     1021 cccttgcttt aataccaatg cttatattat ggtttggtat aggagaggaa tcaaaaatta
     1081 taattatagt tttagcatcc tttttcccaa tctttacaag cacattgaaa gggattaaaa
     1141 attgtgactc aaaactgatt gaagtaggta gagtgtttga gttttcaaag cttcagataa
     1201 tatttaaaat aataatacca aatgccattc ttgatattgc tgttgggtta aagttagctc
     1261 ttggatatag ctttagagca attattgggg cagaacttgt agcagcttca tctggtcttg
     1321 gatacttgat atctgatgga aaagaaatgt ctagaacaga tgttgttatt gtaggtataa
     1381 tcgtaattgg acttttagga ataattacag attacatatt ttcgattatt gtgaagaagg
     1441 taagtaaggg aaagatggtg gaagcatatg aataatttag tatgtgactc tggatatata
     1501 attagaaatt tatataaaag ttatgaagtt gacaatgaag aacatgtagt tcttagtgat
     1561 atctctttaa acatagatag aaagcatata acagtaattt taggtgaaag tggttgtgga
     1621 aagactacct tgcttagagc aattgcatca cttgaaggta taaatagtgg tgaaattagg
     1681 tttattaatg ataataaaga gtatagacct aatgttggtt ttgtttttca ggagagtaga
     1741 ttgatgcctt ggttaaacgt aagtgaaaat atagcttttc ataatcaaag aaaaaatact
     1801 attttaaaca aggtgcttag aaaatttaaa cacaataaaa gaattattgg taaaatgata
     1861 aatgataatt ttagaagctt agaaacaaat gataatttga acaaatcaaa taaatataat
     1921 aggaaaagta atattgatgt agaaaaatat cttaagatga tgaacttgca gaaatttaaa
     1981 aactcttatc caaatgaatt gtcaggaggt atggcacaga gagtaagcat agctagagct
     2041 ctttctttca atccagatat gttgtttatg gacgagcctt tttcggcatt agattatttt
     2101 acaaggattg atatgcaaaa tgaagttata agaatacata agtcaactaa taaaggcgta
     2161 atatttgtaa ctcatgatat tgatgaagca ttaaagatag gcaaaaaaat agttgttttt
     2221 acagacgaaa gaaaaataaa agaatttaat atagaagatg gttataatag agatttaact
     2281 tcagagtact atattaaatt aaaaaaagat attttaatga caatgaaatc aaataaaggc
     2341 atttaaataa attaataata ttttaaggag tagagtaaat gaaattaaaa aagctattga
     2401 taggtataac gatattaaca cttgcaacag catctttagc tggatgtgct aaaaaaaata
     2461 atggagaaaa actttcagaa ataaatttaa cttatgtaaa atcaccatta aatgttcctt
     2521 ctataattca aaaacaggat gatttatttg gaaaggaatt taaaaaggat aatataaaag
     2581 ttaactttca cgaaattact acaggtccag agcaaacaca ggctttagct gctggagaaa
     2641 tagacttctt acatgcacta ggtggtacgt cagctttaat agctgcatct aatggagtag
     2701 agttaaagat attaaacact tatagcaggt caccaaaagg atttatgatt ttgactaata
     2761 ataattcaat aaaatctgca gcagatttag taggtaagaa ggtggcaggt ccaaaaggga
     2821 ctatattaca tcaggtattg atttcagcac ttgataaaga aggattaagt atggatgatg
     2881 tggaatttgt taatatgggt attccagaag catcagcagc tctttctgat ggtagcgtag
     2941 atgctgcatt gattgctggt ccagcagctc ttaaagctat gaagtcaggg agtaaacttg
     3001 tagcaaatgg tgaaggttta gtagatggta taatagtaac tgctgttagc acagactttg
     3061 cagagaaaca tccagaaata gttgaaagat ttatgaaggt tgaaaaggaa actttagaat
     3121 atgtaaataa taattttgat gaagctatgg aaaaagtagc taaagaagtt gatttaagtc
     3181 ttgaagaaac taaagaatta tatgcttggt atgattttag tttagatata acagataaag
     3241 acatatcttc gcttgaagat acacaagact tcttaataaa aaataagtta caagagaaaa
     3301 aagtgaatat aaaagaatta atctataatg ctaagtagaa actatgctaa aataatacta
     3361 agtaagaaat ataataaaag tttaagttac cttattaagg taactttttc tattttaaat
     3421 tagtaatttc aatttatttt agaatgacta attgataata atgattaatt aatcataaaa
     3481 agatactttt ttatagggtt ttattaagat gtctaataat atagttatac ttatgaagtc
     3541 agtaattgtg aagttagcaa tatgattaaa tttatatatt gtaaaaagat tataatacat
     3601 taaaattgaa cttttatatg atgagtaaat aggtatatta ggaggaatta tatgagttat
     3661 aaagtatata ttgtggaaga tgatatatca ataagtacat tacttaaaga ttatattgag
     3721 aaatatggat ttgaagtgat tgttgaagaa aactttgatg atataatgat tacatttgaa
     3781 agttttaagc cacacttagt tctattagat gtaaatttac ctaaatttga tggtttctac
     3841 tggtgtagat gtattaggca acagtcgaat ttacctataa tttttatatc agctagagat
     3901 ggtaatgtag accaaattag agctttagaa agtggagcag atgattatat aacaaaacct
     3961 ttttactatg atgtagtaat ggcaaaaata aagagtcata taagaagggt atatggtgaa
     4021 tattcaccta aaatagacga aagaatagtg gagctagatg gattaaagtt ttatccagaa
     4081 aggttagaac tagaattaaa tggtgagaat ctaattataa ctaaaaaaga aggtatactg
     4141 attgaatgtc ttatcaagag atacccaaag gtagtaagta gagattatct tttagaaaag
     4201 atatgggatg atattgagtt tgtagaagaa aatactttaa atgtaaatgt tagcaggata
     4261 aggaaaagat ttgaggaatt aggcatagat aacgctgttc agactgtccg agggttagga
     4321 tataaattaa ataagaattg gtaaggagaa agtatgaagt tatttttaaa agattataaa
     4381 ggttatattt ttatatatta tataggtata gttatgacaa ttatttactg caatcttatg
     4441 aagtttatta gttttggaga gagtttatat attcttttat ttagttcttt tatattgtta
     4501 tgttttttaa tatataagta ctataaaaat agagaagtat ataatctgtt tgaaaaagga
     4561 gtacattctt tggaggagtc atgtttgtat ttaggaagct cattcctggg gagaaatata
     4621 tcagttattt taaaacaact acataaattg tatcaatcaa tgatacaaga gcataaaaag
     4681 atacatagtg aacatttaac tttcatcaac caatggattc atcagatgaa aacaccaata
     4741 tcagttataa gccttcagct tcaagattat gagggagaag aagtaactga aaatataaga
     4801 gaagaaatag atagattaaa taaagggtta gatatggcta tgcattttgc aagggcagat
     4861 aattttcaaa aagactttat agttgaaaaa gccaaattaa atgaaattgt atctagtatt
     4921 gtaaatgaag aaaaaaggtt atttataaaa agtgggatga ttcctaagat tcaaatagac
     4981 aagtttactt atgtttatac agatgtaaaa tggatgaaat ttataatagg tcaattaatt
     5041 acaaatggag tgaaatattc taaagattat agtaactacc taactataaa atctgaagaa
     5101 aataataagt atgtaatttt aagtataata gatgaaggaa ttggaattgt ttctaaagat
     5161 ttaaaaaggg tatttgaccc attttttaca ggagaaaatg gtcgtaagtt tggagaatct
     5221 acaggtatgg gtctttatct agtaaaaaag gtatgtgatg atttaggtca taaagtaagt
     5281 ataaagtcag aagtaggaaa aggtacagaa gtttcaatct cctttaataa agatgtaaat
     5341 agtttaataa aggtgtaaat agtttgacaa agatgtaaat tatctgttac aatagggttt
     5401 ttagttaatt gtactttttt attaaacaaa atattcatag ttaaataatc caaatttaaa
     5461 agaagttatt tgcaaacctt acatagatgt aaggtttgtg taatgtttgc agatagttaa
     5521 attttctaat ctaaattaca attaatataa gaaataacat aagggaaata acataaaaaa
     5581 gaaattttgt aaaaaaggaa ggatgtttat tgatgagttt agatgttatt tttatattga
     5641 aatcagtcat aatagctata gtagaggggc ttacagaatt tattccagtt tcatctacag
     5701 gtcatatgat tttggttggt aatttaatag attttaaggg gcaatttgct gaaatgtttg
     5761 aggttgtaat acaacttgga gctatattag cagtggtagt actctactgg aaaaagataa
     5821 aagatagcgt tatagaattt tttaaattta tatttactgg tggaaaagaa ggaaagattg
     5881 gttttagatt tggaatgaat gtaattatag gttgcatacc atttgcgatt ataggagttc
     5941 tattttacga taatataaaa tcattgttta atttacaatc tgtaattata ggatttatag
     6001 tgggtgggat tttattatta gtagtagaaa ctttgtttag aaaaaagaat cattccacag
     6061 ataatataga taaaattact ccaatacaag ctttaaaggt tgggacatta caagttttgt
     6121 ctgcatggcc tggtatgtca agaagtgcat ctactattat gggtggatgg atagcagggc
     6181 ttaattctcc tacagcagca gaattttctt tctttttagc tgtaccagct atggtggcat
     6241 cttcaggaaa agatttattt gaatttgatt attcaataat gacaccaaca ttgtggattg
     6301 ccttggtagt tggatttata gtagctttta ttgtatcaat aatagttatg gaaaaattcg
     6361 tgaatttttt aaagaaaaaa ccaatgagag tgtttgctat atacagaata atcatgggtg
     6421 tagtacttgc agtattagct tttactaaca taattagtgt atagaaaaaa tagatttatt
     6481 atctatagaa attaaactaa gataaattat aattaacaat tatatatgtt atttcaaaaa
     6541 ttagattagg aaggatagtt aaataatgaa aattttaact gtaaataatt taagtaaagt
     6601 acatggtaaa aaaataattt tcaatgcatt aaatgacata aattttagta tagaatatgg
     6661 agaatttgta ggaattatgg ggccatctgg cagtggaaaa actactcttt taaatatgat
     6721 ttctactatt gacaagccaa ctacaggaac tatggagcta aagggcaaga atcttttatt
     6781 gttaaaaggt gaagaacttg cattatttag aagaagagaa ttaggttttg tatttcagga
     6841 ttttaattta ttggatacct taactatagg agaaaatata gtattaccat tgacattaga
     6901 taaggtcagt gtaaaagagc aagataagag gttgaatgaa gtatctgaaa ttttaggaat
     6961 aaaagattta ttaggaaaaa gaacttttga agtttcaggt ggtcaagccc agagaactgc
     7021 cattgctaga gcattaatta acaaaccatc aatactttta gcagatgaac caacaggtaa
     7081 tttagattca aagtcgtcaa agactgtaat ggagctattt caaaagataa ataaagaaaa
     7141 taaagttacc actatgatgg taactcatga cccattagca gcaagttatt gtagtagaat
     7201 tttattcata aaagatggag ctatatataa tgaaatatat aagggaagta gtagagagca
     7261 gttttatcaa gagataatgg atgtacttac attattggga ggagataatt aatgaatata
     7321 aaacagtttg ctattaacaa tatttcaaga aatattaaag catatttagg atatttagca
     7381 agtattatta tatcgtcttc actactattt tcttttatta tgtttatagc tcatccggac
     7441 ttagatgtat ctcaatttcc tgattattta aaagaaggtt ataatatgag taagataatt
     7501 gcatatttgt ttttattctt ctttgtattt tattcagtta gtgtatttct taaaagtagg
     7561 tataaagaat ttggaatact ttatattaca ggtatatcaa aacgccaagt aatgaaatta
     7621 atatttatag aaaatatgat aataaatata gtatcttctg taattggaat aataataggc
     7681 ttaatctttg caaagatatt tttagtggtt atgtctacat tcttagaatt atctccatta
     7741 aaattctata taccatttaa tgcgatattg tctactttaa tctattttat gattttagct
     7801 gtattaacat caatatttac ttcttttata attaaagaaa atcaggtgtt gaagttactt
     7861 aaaggtacaa aaactccaaa accagagcct aaaacatcac taatgcttgc tatattaagt
     7921 atttgtttgt ttataatagc gtattataat gctgtcacat caactgacca atatacaaca
     7981 tacctgcgct tagttccagt tacatctctt actatagtag ctacctactt atttttttct
     8041 caatttagtg tgttttttat aaagaagcta aaattaaaca agagatttta tagaaaaaat
     8101 acaaatatgc tttggatttc aaatttgatt tataaggtaa aggataacgc tagaatattt
     8161 ttcttgataa ctataacctc tgcagtagca tttactgcta taggtacagt ttattccttt
     8221 tggaaagatg taaatcgtca aattaattta atatatccta atacaatata ttattcaaca
     8281 atgaccttgc ataatgaccc taaaaagcct gatagcaaag aaaaagacaa ggaaagaatg
     8341 agatttatag aaaataatct aaaaaaagag aatatagact atacaagaat taatggtgaa
     8401 tttaaaactg tatttcccaa gaaaagtgat tttaatgtca gaattatgaa agaatctaag
     8461 tacttagaaa taacagaagt tataggggtt aagcccataa gttttgaaga taatgaatct
     8521 atatcattat tatcatcaag tttaccagga aataagaaaa taaaagaaaa tgttattgta
     8581 gggaatagaa gcttaaaggt ggtaaaacaa gtagaagagt gtgttatgcc agcatattat
     8641 aaaaatatgt atgtggtaaa ggacaatttt tatgatagta tagaatcaaa atttatagtg
     8701 gatagattta ctgcatttga agtagaggat tcaagtaaaa ctatagagat atgcagagaa
     8761 tttgaaaata aatttaataa tgagtcaggg atgcaaccat atttgttttt ctctaaagaa
     8821 ttaatgatag aaactggtaa attaatgtat tctacattta tatttttagc aatatttata
     8881 ggtttaattt tctttgttac tacaagtagt tttttatata ataaatttta tatggattgc
     8941 caagttgata agaaaaagta tgaacagtta aataaattgg gtatgacata taatgagatt
     9001 aaaaaagctt caacgattga gattggaata gtatttttgt ttccttatat agtggctgtt
     9061 atacattcca tatttgcact tactgcactg aaaaattctt ttgatataga agtagcatca
     9121 tcagctgttt tggtaatggg aagcttgttc ttggttcaaa tagtttattt tttaattgta
     9181 agaaataatt atttaaaaga gataaggtta aatttagtca atttttagtt actattatta
     9241 aatgataaca aaaagggttt aaaattaatt aatattactg gaattataga tgtttatttt
     9301 ttcagtaata ttttttattg tattaactat taaaattttg tacaaatata aaatataata
     9361 tgataaatgt caaaattata aaaaaattct acttatataa aatataaaga ggttatatgt
     9421 acatatatgt agaaataaat aataaaaata tcaattaatg ttattagggg gggttactca
     9481 ttagtaaatt agctaataca cttagaatga tgatattaat tcaaagcaga ggcaaaatga
     9541 aatgcaaaga attatcagaa gagttggaag taagtgaaag gcaaataaaa agttataaaa
     9601 tgtacttaga gcaagctggt atatttataa attcaacacc tgggatttat ggtggttatg
     9661 aaatagataa atgtaatagc atctcattaa ttaaattatt agatagtgaa gtttcaattt
     9721 ttatattaac taagtggtag ataagtagaa tattagcaat atcaacgagt atttagaaac
     9781 ttgttttata ttaataaagc agtatataaa attgtagaat caacaatagc atatactaaa
     9841 acatcaacct tatattaact aaatcatatc taaataaaat acacttacct ataaacatta
     9901 taaaatcaat acaaaaatga ggtgaaacaa aatttatgat aaggaaatta aacaataaag
     9961 acataaataa aataatggaa atatgggaaa aaagtacaat caaagcacat gactttataa
    10021 gtaaagaata ctggcaagat aactataatg ctgttaaaaa cgaatatata cctatatcag
    10081 atacatttgt atatgatgat ggagatgaaa taaaaggatt tataagcata atagataaaa
    10141 gctttatagg agctttattt ataaaaccca aataccaaaa tataggtatc ggaggtaaac
    10201 ttttagatta tgcaactaaa aaatataaaa gtctaagctt agcagtatat aaagataata
    10261 aaaaagcagt tgtgttttat aataaaaaag gttttaatat agtaaaagaa caagtaaatg
    10321 aagattcagg atttaaagag tacataatgg aatatagtaa ataatatgag tacattctat
    10381 taaaagtata attaatttat ttgccatttg attaggagaa tttttataag agtaattttt
    10441 aaaatagact atatgaagat aaagatattt attatagatg taagttaatc agaattgata
    10501 tagttacaaa agaggaaaat aaaattgtat ggtgtatttc agatttaaca aatttactat
    10561 ttaaagataa taagtttgat ataatattaa agatactttc acctataaac tataaggtat
    10621 ttgaaagagt tttaaataat tatggaatgc tgataaaggt ttactaaaat ttaataatta
    10681 ttagattata gaaaattgcc atgaaattag ttcggtaaga actagtttca tagcaatttt
    10741 aattcttata agtgtattta tttttacaga tttaaaaaac ttatacagta tctaataatg
    10801 taatcttatt actttagcct ataaatattt aaatcttaaa atgttctctt tgagataagt
    10861 ttatttcaga gtcacaattt ccacaacagt aagatgttga gacatcttta aatttttcag
    10921 tattctcatc aaagttcatt tttttctttg caataattcc taccttagtt aaattagaac
    10981 cacaattatt acagttttga attttgcata ggttatatag tttatcaagt gtaatatctt
    11041 ttaataattc atcacaatta caacatctga tagaagaaac ttcactacac atttcaatag
    11101 gttcagcttc tttagaagaa aatttatatg ttatgtcatt tgatacttta taacctgtat
    11161 caaatatatt agaattacaa cttggacata agtactttgg aagatatata tctttttttt
    11221 caaaagtatc actactaata ttacagttta attcatctaa aattggtctt tcatctaggc
    11281 tataaccttt atttaagctt agaagataat tataaatttt tttatcttca ttctgttttg
    11341 atgtatctaa caaatgctct attatattag tgtatttttt gttttttgaa ggcatgttta
    11401 aatccagata attgttttca atagcaaagc atactatgtt gaaatagtta tatttaactg
    11461 caaaaaataa taacatttca tcaaatactt tatctattat tttgtcagaa atacttatcc
    11521 aaaaatctgg tgactctaag taaactttcc tactaagttc atctaactct tcatctatat
    11581 tatttatatt attttcatta actttcatat catcacttcc tt
//
LOCUS       DAEPXF010000122        11348 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain HB27
            SAMN13376389-rid12479423.denovo.128, whole genome shotgun sequence.
ACCESSION   DAEPXF010000122 DAEPXF010000000
VERSION     DAEPXF010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13376389
            Sequence Read Archive: SRR10518432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 11348)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11348)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001069624.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001069624.1
            Genome Coverage       :: 315x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/15/2021 01:54:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,165
            CDSs (total)                      :: 4,076
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 89
            rRNAs                             :: 10, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 10 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 19 of 69
            Pseudo Genes (incomplete)         :: 44 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 10 of 69
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11348
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13376389-rid12479423.denovo.128"
                     /strain="HB27"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="China: Hebei"
                     /collection_date="2017"
     gene            201..1034
                     /locus_tag="KRL67_002722"
     CDS             201..1034
                     /locus_tag="KRL67_002722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase"
                     /protein_id="HBH1523588.1"
                     /translation="MRNKKKKINRKKLYLLLSAIVVCLAFIAVGVRVSSLNTKENEIS
                     RSAKLSSSTITDIVSNTSIEVSKGPSKSSGKIAYITIDDGPSKFTDQMIKTLNKYNVK
                     ATFFMIDGNMKEYPQQVKNIIKNGNTAGFHSVSHDIHKLYVTSTSAKEEFDTNDQTFY
                     KITGKHSKVIRIPYGSKPYTPQASYQALVDAGYKIWDWDLDTEDWRSNSSQIVQNVKN
                     HIKNRKGEDKDQLVVLMHEKKQSAEALDSVLKFLSDEGYEFAPVDQNQIPKNYWLRNL
                     E"
     gene            1127..2059
                     /locus_tag="KRL67_002723"
     CDS             1127..2059
                     /locus_tag="KRL67_002723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HBH1523589.1"
                     /translation="MVGILSLMMVGCNKSLAKVNDVEITKEQYKKTKAVLSATNNYIN
                     GQSLDELEKTLDKKGRNKLENVIISFMVDNELLYQEAKDKGLTPSKSEVDSKYQELED
                     KMNLNTSYKEKMDKAGVDKEYLKQEISRDLAIDKNKKAFEDRINISDNDMEAYYTSHK
                     KDFNVEEVSASQILISTLDKNKKEVSKDKKEALKKKADNILTKIKNGESFESLAKKYS
                     DDKATGKNGGQLGYFSKDDKNAEFTKEVFKLKKNEVSNVFETSYGYHIVKVTDKRERQ
                     KSFNECQSLIRESILNEKYIEHIKKLNEDAKIDR"
     gene            2111..2593
                     /locus_tag="KRL67_002724"
     CDS             2111..2593
                     /locus_tag="KRL67_002724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HBH1523590.1"
                     /translation="MFKSLKRTKVEKYIIDNKDSFYRIAYSYTKNEEDALDVVQEAMY
                     KALYSVENIKEVNYIKTWFYKILVRTSIDFIRKNRKYNNMTDIDLVDETGEYDKYTDL
                     DLRRALEELPIEYKSIIILRFFEDLKIEEVAIILDENVNTVKTRLYTALKKLKLKIEE
                     "
     gene            2605..3474
                     /locus_tag="KRL67_002725"
     CDS             2605..3474
                     /locus_tag="KRL67_002725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3298 domain-containing protein"
                     /protein_id="HBH1523591.1"
                     /translation="MTSKERLNKLKDEYHKIPIPKKLDTIINHEKIENIYREKNKKVN
                     RLRFKIAIAFACIFTVLVNISPVFADNFSKIPVIGAIVEVITIKNYSLKSENYEAEID
                     IPKIQGLKDKNLEQRLNSSFMEDGKRLYHQFQERMEKIQSSKNKGYKSLSLSYSVKNN
                     SKKFLSIEMTKNEIEASSYVSKIHYTIDKKRQIVLTLPMLFKDDKYIKVISDNIKEQM
                     REQMKKDSTKSYFIDQKKDLPVEDFKAINKYQDFYFNKNEDLVICFDEYEVAPGYMGA
                     VEFVIPYKVIKDL"
     gene            3508..4233
                     /locus_tag="KRL67_002726"
     CDS             3508..4233
                     /locus_tag="KRL67_002726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3298 domain-containing protein"
                     /protein_id="HBH1523592.1"
                     /translation="MFKIAIVSICLLVSLVDINPIFAGSSSNETSNSDTIKVLNAKSL
                     NFKQGNYEVKVSIPKIKGLKDKTLENKLNNEFLQYGEKIYSEFQNKMKKENNKGHRYL
                     NVIYNVKNNSKDFLSIEMIKEEIEASSSVTKKHYTVDKNREILLNLPIFFKDSRSIRV
                     ISDNIKEQMRNQIKKDDTKSYFIDQDKDSSMKDFESINKYQDFYFNKDGNLVISFDEY
                     EVAPGYMGAVEFVIADKVIKRLR"
     gene            4275..5096
                     /locus_tag="KRL67_002727"
     CDS             4275..5096
                     /locus_tag="KRL67_002727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XdhC family protein"
                     /protein_id="HBH1523593.1"
                     /translation="MNIYEQAIRLIENNEDFAFATITSHSGSTPRETGAMMIVKNDST
                     IFGSVGGGSVEAECIKHAINVIKNRESMLYKFTLNKSDVAKLGMICGGTGEIQIDFID
                     SKLKSNIEKFNKRLKENTSKAYIFGAGHISRDVAVILSLLEFRTVVIDDREEFANHER
                     FPDSEVVVLDSFENIPDFPTDENSYIIILTRGHLYDSSALEWALKRENGYIGMIGSRT
                     KIGLTYEKLMKKGFKKEELSKVHAPIGIKLNAQTPAEIAVCIAAELINCRANKEK"
     gene            5101..5862
                     /locus_tag="KRL67_002728"
     CDS             5101..5862
                     /locus_tag="KRL67_002728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HBH1523594.1"
                     /translation="MNTGSKDIDTLNVNFRGIEIKAVLVYKNRKNISIKIDPFGKIIV
                     MSPPKISKKIIKDIIIEKGDWILKKSEKYKGREEVYKQRMFITGEKFLYLGEEYHLVI
                     KELLKDSVKKSNCRITINEYKIIVQTNDTSTDFIKKSLKSWYKMESERVVLERIDFLK
                     LNCEIMKQLVPASVKVKEQNKRWGSCTSQKNIYINSKISMARLDVIDYVIVHEFSHLV
                     HMNHSKKFYDLVKTIMPDYKDKENWLKINGYKIII"
     misc_binding    6022..6279
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            6392..6631
                     /locus_tag="KRL67_002729"
     CDS             6392..6631
                     /locus_tag="KRL67_002729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HBH1523595.1"
                     /translation="MENTVYAKLNQGIDSFLRVAMTLRRREVDIQSISMTVDNMNNSG
                     IKLIVNEEKTSLDSVLNHMKKLHDIREITVERMRH"
     gene            6935..7930
                     /gene="ilvC"
                     /locus_tag="KRL67_002730"
     CDS             6935..7930
                     /gene="ilvC"
                     /locus_tag="KRL67_002730"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HBH1523596.1"
                     /translation="MKMARMYYEKDVDLEVLKNKKVAVLGYGSQGHAHAQNLRDNGVH
                     VVIGLYDGSKSAQKAKEDGFEVKSVAEATKESDLTMLLMPDEKQKKVYEESVKDNLKE
                     GQTLVFAHGFNIHYNQVQPPEFVDVVMVAPKGPGHLVRNVFTKGSGVPALFAVYQDYT
                     KKATETVLAYAKGIGATRAGVLETTFKEETETDLFGEQSVLCGGISELIKLGYKTLVD
                     AGYQKEVAYFECLHEMKLIVDLIYEGGFERMRYSISDTAEYGDYVSGKRVITDAAKQG
                     MQNVLEDIQNGKFAKAWIKENEEGRENFLKIREEEYNTEIAEVGRSLRSMMSFLK"
     gene            7983..9671
                     /gene="ilvB"
                     /locus_tag="KRL67_002731"
     CDS             7983..9671
                     /gene="ilvB"
                     /locus_tag="KRL67_002731"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic-type acetolactate synthase large
                     subunit"
                     /protein_id="HBH1523597.1"
                     /translation="MNGAKVILECLKKEGIDTIFGYPGGAVIPLYDALYDYSDDFKHI
                     RTSHEQGLVHAADGYARSTNTVGVCFTTSGPGATNAITGIATAFMDSSPMLVISGQVP
                     TSLLGKDSFQEIDITGATLSMTKHNYLVRNTKELVPTIKEAFRVANSGRKGPVLVDVP
                     KDLFLAEMDFSGEDYDLCQIDDYMDYKSDFGLDDETNTKLLNEAIDIIKESKKPVIYA
                     GGGVKSSDSEEILEKFATKIDTPVLNTLMGLGNIDRKNELSLGMVGMHGSRESNLALS
                     NSDLVIAIGARFSDRVISKSSEFAKNAKIIHIDIDPSEISKNIESNVSLVGDVKLVLS
                     LLLEMVESKNNSNWKEEIKTFRKSEGIQTDEFHPQNILKKINEKYETLKKPTVVVTDV
                     GQHQMWAAKYWNFKGNKSFITSAGLGTMGFGLGAAIGTKAGNVDKNVVLVTGDGSFRM
                     NCNELATVANYNVPMLILLFNNRTLGMVRQWQKLFSNQRYSQTDINENVDYVKLVNAY
                     NIDGYKVSNMEELGKTIDMIDFNKPVFLQCDIDKDYDVYPIVAPNDALENLICN"
     gene            complement(10029..11159)
                     /locus_tag="KRL67_002732"
     CDS             complement(10029..11159)
                     /locus_tag="KRL67_002732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HBH1523598.1"
                     /translation="MKGGVRKRSNKWYYYFDLGIVEGKRKKVERVGGNTKKEAEKALR
                     EALNEYENSGIVFEESNISLSDYLDFWYKEYVLLNCKYNTQESYRINIEKHIKPKLGA
                     YKVKALTPAILQNFINRKYKEDYSQNTLQVLKAILHRSLKSAVHPYKHIRENPMQYVS
                     IPKTKSKTETNKVKTITLEEFNQILNIFPQDSFQRIVLLIGFHTGMRRGEIIALKWDN
                     IDLDNKTITVKHTLIKKPNGMFELGQPKTESSCRTIFTGDTLIKALKEHKLYQKKMKL
                     KYGEFYFDSDWVCTKENGQQVNTHTLDTIVRQIRVALNNDFHFHSLRHAHATLLLENG
                     ANIKDIQNRLGHSQLSTTMDTYSHVTDKMKNETVDIFEKITN"
BASE COUNT         4611 a         1169 c         1960 g         3608 t
ORIGIN      
        1 ctatataata ctcaattttc aacattctat acaattatac agaaattata cagcataata
       61 atcaccgaaa taacaaattt taaaaattaa gcaaaacttt ttgtcgaagt tcaacgtcta
      121 tatatatgta gtgtaaattt ttgggataaa atactacaaa aatataaatg taaaaatata
      181 gtaatattgg aggtcgaacg atgagaaata aaaagaaaaa aataaataga aagaagttgt
      241 atttgctttt aagtgctatt gtagtatgtc tcgcttttat agcagttggt gttcgcgtta
      301 gctctttaaa tacgaaggaa aatgaaatta gtagaagtgc aaaactgtca agttctacta
      361 ttacagatat agtatctaat acatcaatag aagttagtaa agggccaagt aaaagttctg
      421 gaaaaatagc atacataact atagatgatg gtccatctaa atttactgac cagatgataa
      481 aaactttaaa taaatacaat gttaaagcaa ctttttttat gatagatggg aatatgaagg
      541 aatatccaca gcaagttaaa aatataatta aaaatgggaa cacagcaggt tttcatagtg
      601 tatcacatga tattcacaag ctttatgtta ctagtacatc agcaaaagag gaatttgata
      661 caaatgatca aactttttat aagataactg gtaaacattc aaaagtaata agaatacctt
      721 atggaagcaa accatataca ccacaagcct cttatcaggc tttagttgat gctggatata
      781 aaatttggga ctgggactta gatacagaag actggagatc taattcttct caaattgtac
      841 aaaatgtaaa aaatcatatt aagaatagaa aaggtgaaga taaagaccaa cttgtagttt
      901 taatgcatga gaaaaaacag agtgcagaag ctttagattc agttttaaaa ttcttatcag
      961 atgaaggtta tgaatttgcg ccagtagatc aaaaccaaat accaaaaaat tattggttac
     1021 gtaatttaga gtagttaaaa tataagaata ttgattgata tttttagaaa ttgagaataa
     1081 aaatgagtga ggtgtaggta tattgaataa aaaactttat ataggaatgg tcggaatatt
     1141 gtctttgatg atggtcggtt gtaataaaag tttagcaaaa gtaaatgatg tggaaatcac
     1201 aaaagaacag tacaaaaaaa caaaagcagt cttatctgct actaataatt atataaatgg
     1261 gcagtcttta gatgaattag aaaaaacttt ggataaaaaa ggtagaaata aactagaaaa
     1321 tgttataata tcgtttatgg tggataatga acttttatat caagaggcta aggataaagg
     1381 actaactcca agtaaaagtg aagtagactc aaaatatcaa gaactagaag ataagatgaa
     1441 tttgaataca agctacaagg aaaaaatgga caaagcaggt gttgataaag aatatttaaa
     1501 acaagaaata tctagagatt tagcaataga taaaaataaa aaggcctttg aagatagaat
     1561 aaacataagt gataatgata tggaagccta ctatacaagt cataaaaaag attttaatgt
     1621 agaagaagta agtgcttctc agatacttat atcgacttta gataaaaata aaaaagaagt
     1681 aagcaaagat aaaaaagaag ctttgaagaa gaaggcagat aatatattga ctaaaatcaa
     1741 aaatggagaa agttttgaaa gtttggctaa aaaatattcg gatgataaag caactggcaa
     1801 aaatggagga cagctaggat atttttcaaa ggatgataaa aatgctgagt ttacaaaaga
     1861 agtgttcaaa ttaaagaaaa atgaagtttc aaatgtgttt gaaactagtt atggatatca
     1921 tatagtcaaa gttactgata aaagagaaag acaaaaaagt tttaatgagt gtcagagttt
     1981 aataagagaa tctattttaa atgaaaagta tattgagcat ataaaaaaat tgaatgaaga
     2041 tgctaaaata gacagataga atttctaaca gctcaatcct agcctgtaat cggagggaga
     2101 ttttacagga atgtttaaga gtttaaagag aacgaaagtt gagaaataca ttatcgataa
     2161 taaagactct ttttatagaa tagcatatag ttatacgaag aatgaagaag atgccttaga
     2221 tgtggttcaa gaagccatgt ataaggcact atactcagtg gaaaatatta aagaagtaaa
     2281 ttatataaag acatggtttt ataaaatttt agttagaacg agtatagatt ttattagaaa
     2341 aaaccgtaaa tataataata tgacagatat tgaccttgta gatgagactg gagagtatga
     2401 taaatatact gatttggatt taagaagagc tttggaagaa ttacctattg aatacaaatc
     2461 tattataata ttaagatttt ttgaagattt aaagattgaa gaagtagcaa taatattgga
     2521 tgaaaatgtg aatactgtta aaactaggtt atatactgct ttaaaaaaac taaaattaaa
     2581 aattgaagag tagggataaa aagaatgaca agcaaagaaa ggttaaataa gttaaaagat
     2641 gagtatcata aaattccaat acccaagaaa ttagatacta taataaatca tgaaaaaata
     2701 gaaaacatat atcgagagaa aaataaaaaa gtaaatagat taagatttaa aattgcaata
     2761 gcatttgcat gtatatttac agtattagta aatataagtc ctgtatttgc agataatttt
     2821 tctaagatac ctgtaattgg agcaatagta gaagtaataa ctataaaaaa ttatagttta
     2881 aaaagtgaaa attatgaagc tgagattgat attcctaaaa tacaaggttt aaaagataaa
     2941 aacttagaac aacgattaaa cagtagtttt atggaagatg gaaaaagact ttatcatcaa
     3001 ttccaagaga gaatggaaaa gatacaatca tcaaagaata aaggatataa atctttaagt
     3061 ttgagttata gtgtaaaaaa taattctaaa aaatttctat caatagagat gacaaaaaat
     3121 gagatagagg cttcatcata tgtgagtaag atacattata ctattgataa aaaaagacaa
     3181 atagttttga ctttaccaat gctttttaaa gatgataagt atataaaagt tataagtgat
     3241 aatataaaag aacaaatgag agaacaaatg aaaaaggata gtacaaaatc ctattttata
     3301 gatcaaaaaa aagacttgcc tgttgaagat tttaaagcaa ttaataagta tcaggatttt
     3361 tattttaata aaaatgaaga ccttgtaatt tgctttgacg aatatgaagt agcacctggt
     3421 tatatgggtg cagttgagtt tgtaatacca tataaagtaa taaaagattt gtaaattgaa
     3481 tagtagttta tatataaata aaaatctata tttaaaattg ctatagtttc aatatgctta
     3541 ttagtttcac tagtagatat aaaccctata tttgcaggta gttcctctaa tgagacttca
     3601 aacagtgata caataaaggt attaaatgct aagagtctca actttaaaca aggcaattat
     3661 gaagtgaagg ttagtattcc taaaataaaa ggtttaaaag ataaaacttt agagaataaa
     3721 ttaaacaatg agtttttgca atatggggaa aaaatttaca gtgaatttca aaataaaatg
     3781 aaaaaggaaa ataataaggg gcatagatac ctaaatgtaa tctataatgt caaaaataat
     3841 tctaaagatt ttttatcaat agaaatgata aaagaagaga tagaagcttc atcatctgta
     3901 actaagaagc attacactgt tgataaaaat agagaaatac tattaaattt gccaatattt
     3961 tttaaagata gtagatctat tagagttata agtgataata taaaagagca gatgagaaat
     4021 caaattaaga aagatgacac aaaatcttat tttatagacc aagataaaga ttcctcaatg
     4081 aaagattttg aatctataaa taagtatcaa gatttttatt ttaataaaga tggaaatctt
     4141 gtaattagtt ttgatgaata tgaagtagca cctggttata tgggagcagt agaatttgtg
     4201 atagcagata aagtaataaa aagactaaga tagatatata gaagattttt agatagactg
     4261 gtaaggagaa ttatatgaat atatatgaac aagcaataag attaatagaa aataatgagg
     4321 attttgcatt tgctacaata acatctcatt caggttcaac accacgtgaa actggagcaa
     4381 tgatgattgt gaaaaatgat tctactatat ttggctcagt aggaggaggt agtgtagaag
     4441 cagaatgtat aaaacatgca attaatgtta taaaaaatag agaatcaatg ttgtataaat
     4501 ttactctaaa caaatctgat gtggcaaaac taggaatgat atgtggaggt actggagaaa
     4561 tacaaataga ttttatagat agtaaattaa aaagtaatat agaaaaattt aataagagac
     4621 taaaggaaaa tacaagtaaa gcgtatatat ttggggcagg tcatatttca agagatgtcg
     4681 ctgtgatact atctctttta gaatttagaa cagtagtaat agatgataga gaagaatttg
     4741 ctaatcatga aaggtttcca gactcagaag tagtagtttt agattctttt gaaaatatac
     4801 cagattttcc aactgacgaa aatagctata taattatttt aacaagaggt catctatatg
     4861 attcaagtgc tttagaatgg gctcttaaaa gagagaatgg ttatataggt atgattggaa
     4921 gtagaacaaa aattggattg acgtacgaaa aactcatgaa aaaaggtttt aagaaggaag
     4981 aattatcaaa agtacatgca ccaataggca taaaattgaa tgctcaaaca ccagctgaaa
     5041 tagctgtatg tatagctgct gaacttataa attgtagagc taataaggaa aaataaacca
     5101 atgaacacag ggagtaaaga tattgatact ttaaatgtta attttagagg gattgaaata
     5161 aaagcagttt tagtatataa aaatagaaaa aatatttcta taaaaataga tccatttggc
     5221 aaaataattg taatgagtcc tcctaaaata tctaaaaaaa taataaaaga tataattatt
     5281 gaaaaaggag actggatact taaaaagtca gaaaaatata aaggaagaga agaagtatac
     5341 aaacaaagaa tgtttataac tggcgaaaaa tttctttatt taggtgaaga gtatcatcta
     5401 gttataaaag aactgttgaa agattctgtc aaaaaaagta actgtagaat aactattaac
     5461 gaatataaaa taattgttca gacaaatgat acaagtacag attttattaa aaaatcttta
     5521 aaatcttggt ataagatgga aagtgaaaga gtagtattag agagaataga ttttcttaaa
     5581 ttaaattgtg aaataatgaa gcaattagtt ccagcaagtg ttaaggtaaa agaacaaaat
     5641 aagagatggg gaagttgtac atctcaaaaa aatatttata taaactcaaa aatatctatg
     5701 gcaagattag atgtaattga ttatgtaata gtacatgagt ttagtcattt agtacatatg
     5761 aatcactcta aaaaattcta cgatttagta aaaaccatta tgcccgatta taaagataaa
     5821 gaaaattggt taaaaataaa tggatataaa ataataatct gaaaacgatt attccaaaat
     5881 ataggtgtaa agtataaaaa aagtattatt gtgaaaaaaa taaataatct gaaaatttta
     5941 aaaaagccat tgacattgta aatgataggg aatataatct atattaacaa caaaacagaa
     6001 ttaaaatata aaattaaaaa attaaaagca atgacagaga caacaatact aatagtgtta
     6061 tccacagaga accaatgttg ctgagaattg gtgttaacag tagtataatg atcactctgg
     6121 agcttttgta ctgaatattt ttagtaagta caaacgtctg tcaacgttat atgactaagg
     6181 ttaaggtaat taattacccg acctgaaaaa agaggggtag atgtattttt tatatctaca
     6241 aataaggtgg taccgcggaa tataactttc gtccttataa gtaatattta tattgcttat
     6301 aaagatgaaa gtttttttat tttaagcgat ataaataaat ttaatccata taaaattaat
     6361 aaaaaaataa taaaaataag gaggaatttt tatggaaaat actgtatatg ccaaattaaa
     6421 ccaaggaata gattcttttt taagagtggc tatgactcta agaagaagag aagttgatat
     6481 acaatcaata agtatgactg tagataatat gaataattca ggtataaaat taatagtaaa
     6541 tgaggaaaag acatctttag attctgtctt aaaccatatg aaaaaactac atgatataag
     6601 agagataact gttgaaagaa tgagacatta attaaatata ttttataaag ctaaagataa
     6661 atatgcttat tttaaaataa aaaactaaag ataaatatta aataagaaaa atcttagact
     6721 aaaatacaaa tgtcattgaa tttatcagtt tatcacagtt aataaattta ttagtttgtc
     6781 gtagctaata aaaactgaaa attctaaaat tataattgta agattatata aatttagaat
     6841 ttaaatctgt tgaataatat taaaacaatg ataatgagta tgaatttcta ataaaaataa
     6901 atatcataaa aataagaaaa agtattggga ggaaatgaaa atggcaagaa tgtattatga
     6961 aaaagatgtg gatttagagg ttttaaaaaa taaaaaggtt gcagttttag gttatggaag
     7021 tcaaggacat gcacatgcac aaaatcttag agataatggt gtacatgtag tgataggtct
     7081 ttatgacgga agtaaatcag cacaaaaagc aaaagaagat ggatttgaag ttaagagtgt
     7141 agcagaagct acgaaagaaa gtgatttgac aatgctgtta atgcctgatg aaaaacaaaa
     7201 gaaagtttat gaagagagtg taaaggataa cttaaaagaa ggtcagacac tagtttttgc
     7261 tcatggattc aatatacatt ataatcaagt acaaccacca gaatttgttg atgttgtaat
     7321 ggtagctcct aaaggacctg gtcacttagt tagaaatgta tttacaaagg gaagtggagt
     7381 tcctgcacta tttgcagtat atcaagacta cactaaaaaa gctacagaga cagttttagc
     7441 atatgcaaaa gggataggag caacaagagc aggcgtttta gaaactactt ttaaagaaga
     7501 aacagaaaca gatttatttg gagaacaatc agtactttgt ggtggaataa gtgaacttat
     7561 aaaattggga tataaaacgc ttgtagatgc aggttatcaa aaagaagtag catactttga
     7621 atgtttacat gaaatgaaac taatagtcga ccttatatat gaaggtggat ttgaaagaat
     7681 gagatatagc ataagtgaca cagcagaata tggagattat gtatctggta aaagggtaat
     7741 aaccgacgct gcaaaacaag gtatgcaaaa tgttttagag gatatacaaa atggaaaatt
     7801 tgcaaaagcg tggattaaag agaatgaaga aggacgtgaa aacttcctaa aaattagaga
     7861 agaagaatat aatacagaaa ttgccgaagt tggtagaagt ctaagaagta tgatgtcatt
     7921 cttaaaataa atatatatta aatggattag tttgagggaa agagtggtgt atagtagtgc
     7981 gtatgaatgg agcaaaggta attttagaat gtttaaagaa agagggaatt gacacaatat
     8041 ttggatatcc tggtggagct gtaatacctc tttatgatgc tctatacgat tattcagatg
     8101 attttaaaca tataaggaca tcccatgaac aaggtttagt tcatgctgca gatgggtacg
     8161 caagaagtac taatacagta ggagtttgtt ttacaacatc tggtccagga gcaacaaatg
     8221 ccataactgg aatagcaaca gcttttatgg attcttcacc tatgcttgta atttcagggc
     8281 aggtgccaac tagtttatta ggaaaagatt cttttcaaga aattgacata actggagcaa
     8341 cattatctat gacaaaacat aattatttag ttagaaacac taaagaatta gttcctacaa
     8401 taaaagaagc ttttagagtt gcaaattcag gcagaaaagg acctgtactt gtagatgttc
     8461 caaaagattt atttttagca gaaatggatt ttagtggtga agactatgat ttatgtcaaa
     8521 tagatgatta tatggattat aagagtgatt ttggtctaga tgatgaaact aatactaagc
     8581 ttttaaatga agcaatagat atcataaaag aatctaaaaa gcctgtaata tatgcaggtg
     8641 gaggtgttaa atcttcagac agtgaggaaa ttcttgaaaa atttgcaact aagatagata
     8701 cacccgtact aaatacactt atggggcttg gcaatataga tagaaaaaat gaactatctc
     8761 ttggaatggt tggaatgcat ggaagtagag aaagtaattt agcactttct aattcagatt
     8821 tagtgatagc gataggtgcg agatttagtg atagagtgat aagtaaaagc tctgaatttg
     8881 ctaaaaatgc aaaaataata cacatagata tagacccatc tgaaataagc aaaaatatag
     8941 aatctaatgt atctttggtt ggagatgtaa agttagtcct tagtttatta cttgaaatgg
     9001 ttgaaagtaa gaataatagt aactggaaag aagaaataaa aacatttaga aagagtgagg
     9061 gtatacaaac agatgaattt catccacaaa atatacttaa aaaaataaat gagaaatatg
     9121 aaacattaaa gaagcccact gtggtagtaa ctgatgttgg tcaacatcaa atgtgggctg
     9181 ctaagtattg gaattttaaa ggtaataaaa gttttataac atcagcaggg ttaggaacta
     9241 tgggatttgg cttaggtgca gctattggaa ctaaagctgg taatgtagat aaaaatgtgg
     9301 ttttagtcac tggtgatgga agttttagaa tgaactgtaa tgaactagct acagttgcaa
     9361 attataatgt acctatgctt attttactat tcaataatag aacattagga atggtaagac
     9421 aatggcagaa gttattttcc aatcaaagat actctcaaac tgacattaat gaaaatgtgg
     9481 attatgttaa actcgtaaat gcatataaca ttgatggata taaagtatcc aatatggaag
     9541 aattaggaaa gaccatagat atgatagatt ttaataaacc tgtattttta caatgtgata
     9601 tagataagga ttatgatgtt tatcctattg tagcacctaa tgatgcattg gaaaatttga
     9661 tatgtaacta aaacttatac tgaatattga taaaattttt tattaaattt ttattggtat
     9721 tcagtttttg tttagagata gagtggcatt ttttattttt gcgaataaat caatgactat
     9781 ataattaaga acttattaaa ttttaattat acaatttaga ataatattac aacttaagta
     9841 aatattaagt tttattcgtt agatttatta ttaatacctt atattagtta tggttgttat
     9901 atactctaaa gccttgaaat tacaagtgta tagctgacta tataaatttg ataattttag
     9961 cttttaaaat agataaatta gattttgcca cccatttgcc accgtattat atttttgggt
    10021 ggcaaactct aatttgtaat tttttcaaat atatctacag tttcattttt cattttatca
    10081 gttacatgtg aataggtatc cattgtagtt gatagttggc tatgacccaa acggttttgt
    10141 atgtctttaa tgttagcacc attttctaat aatagagtag catgtgcatg tcttaaagaa
    10201 tgaaaatgga aatcattgtt taaagctact cgaatttgtc ttactatagt gtctaaagtg
    10261 tgagtattca cttgttgacc attttctttg gtacataccc aatcactatc aaagtaaaat
    10321 tctccgtatt ttaatttcat tttcttttga tataatttat gttcttttaa tgcctttatt
    10381 aaagtgtcac ctgtaaatat agttctgcaa gagctttctg tttttggttg ccctaattca
    10441 aacattccat ttggtttttt aatcaaagta tgctttactg tgatagtttt attatcaagg
    10501 tctatattat cccattttag tgcaataatt tcacctcttc gcataccagt atgaaatcca
    10561 attagtaaaa ctatacgttg aaatgaatct tgaggaaata tatttagtat ttgattaaat
    10621 tcttctaatg taatagtttt aactttatta gtttctgttt tagatttagt ttttggtatg
    10681 cttacatatt gcatagggtt ttctcgtatg tgtttgtaag gatggactgc tgattttaat
    10741 gacctatgta atatggcttt taatacttgt aatgtatttt gagagtaatc ctctttgtac
    10801 tttctgttta tgaagttttg tagtattgca ggagttaaag cttttacttt gtaagctcct
    10861 agctttggct ttatatgttt ttctatgttt attcggtagc tttcttgagt gttgtattta
    10921 cagttaagta agacatattc tttgtaccaa aagtctaagt agtctgataa actgatattg
    10981 ctttcttcaa atactatgcc agagttttca tattcattta gtgcttctct taaggctttt
    11041 tctgcttctt ttttagtatt gcctccaact ctttctactt tttttctttt tccttctact
    11101 atgcctaggt caaagtagta ataccatttg ttacttcttt ttcttactcc acctttcata
    11161 atagtatccc tcccttttag aatgtatgtt tgtttggtgt ttatataaaa gagcagatta
    11221 actgctcttt atatactttt gttatgtaca atattatctt aattttgttt atctggtttt
    11281 cacttaatca ttttcttaat attcttacaa ttttttagct tttatgtgta gtttattata
    11341 ataatatt
//
LOCUS       DAEPXF010000123        11289 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain HB27
            SAMN13376389-rid12479423.denovo.129, whole genome shotgun sequence.
ACCESSION   DAEPXF010000123 DAEPXF010000000
VERSION     DAEPXF010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13376389
            Sequence Read Archive: SRR10518432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 11289)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11289)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001069624.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001069624.1
            Genome Coverage       :: 315x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/15/2021 01:54:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,165
            CDSs (total)                      :: 4,076
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 89
            rRNAs                             :: 10, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 10 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 19 of 69
            Pseudo Genes (incomplete)         :: 44 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 10 of 69
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11289
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13376389-rid12479423.denovo.129"
                     /strain="HB27"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="China: Hebei"
                     /collection_date="2017"
     gene            177..845
                     /locus_tag="KRL67_002733"
     CDS             177..845
                     /locus_tag="KRL67_002733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HBH1523599.1"
                     /translation="MNEISSLINILALTQLSGSNTTNQCNCGYNNSSGFDMIMMTLLK
                     ALSQNNQQTSNGYNLSSNSEKLFNELDSVANNTVGKFIEVDTKDKSVKSRIENAVEQA
                     SKKYNVDANLIKAIIKVESDFNPNTVSSAGAKGLMQLMPENCRDLGVTNPFNIEQNID
                     AGTRHIKEYIDMFGGSIEMGLMAYNGGPGRMRSRGVESISDLYKMPKETQNYIPKVMK
                     YYRG"
     gene            854..1282
                     /locus_tag="KRL67_002734"
     CDS             854..1282
                     /locus_tag="KRL67_002734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaaR family protein"
                     /protein_id="HBH1523600.1"
                     /translation="MGINIDKLNISPSRSIEVKKTKENNKFSDSLNFVKQSSSLEDIK
                     RNLDGVKKAGERLVLTQNYGDISRYKNAVKEYLKSVVDNMYSLDKSSSFWEHQYYKNV
                     KIIDNKLEDMTNKLLSEEKENIDIVSTVDMIQGLLIDMYR"
     gene            1306..1620
                     /locus_tag="KRL67_002735"
     CDS             1306..1620
                     /locus_tag="KRL67_002735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliM/FliN family flagellar motor switch protein"
                     /protein_id="HBH1523601.1"
                     /translation="MNSKKEDIYELNFGQLIEEKTGIIEENQNVFNAKVNVAVSIGNI
                     TETIKNILDLKEGSIIKLNKNVDEKLDIYANDRLFAYGESIITNETLSVRLSKIEDSD
                     IN"
     gene            1771..2046
                     /gene="flgM"
                     /locus_tag="KRL67_002736"
     CDS             1771..2046
                     /gene="flgM"
                     /locus_tag="KRL67_002736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis anti-sigma factor FlgM"
                     /protein_id="HBH1523602.1"
                     /translation="MNIKSVSSNIVVNNYLKNKSDVKKVQQVQKVDKTDKIELSKFAK
                     EMSSVSNMSDEATRNKKIEEIKSALENGTYKIKPEEIAKKMIEEMRG"
     gene            2051..2467
                     /locus_tag="KRL67_002737"
     CDS             2051..2467
                     /locus_tag="KRL67_002737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FlgN"
                     /protein_id="HBH1523603.1"
                     /translation="MITDLKVIIYEEKKIISNMMELLKEQFDYIIEKDLENLNRLNPK
                     LEDISRELASIEIKRRQILGDDVSISEVVENSNDEYLKEIYIDLKHILALAKNQQESN
                     DSLIKKELIFTKKMINFIKPVDKQTTTYNSYGNIRK"
     gene            2485..3795
                     /gene="flgK"
                     /locus_tag="KRL67_002738"
     CDS             2485..3795
                     /gene="flgK"
                     /locus_tag="KRL67_002738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgK"
                     /protein_id="HBH1523604.1"
                     /translation="MAGLFSILNTANSGMSAHQKSIQTISHNISNLDTDGYSRQRTEF
                     ATNSPMYMPSLSNSIGRGQLGTGVHVTDVTRARNSFYDYQFRAEAHKYGNTVSKYDYY
                     NTIETILNEPSDYGISAGIDDFFKGWESLSKDINSDSKKSLVVENASNLATLISESYK
                     KLDKLKGDIDSNIDTEIKQINDMLKSLEGLNKSIDIISGSGSTPNDLLDERDRILDNL
                     SFKLDLENSDVKNMLSDGKLEINELKNVDGTWKTGISGTLQGLFEMHGKIDTYKSDLK
                     DVSDGLAKQINDVYNTSAGITVRDFFITSNVAGEDIIKVNPAIKSNSNELKLTTEEAS
                     KIAKLKDEKIDIGVAGGKVSTISDHYKAFAESVGLDSQKVNQDEVNQRKIINNVDNSR
                     MSVSGVSLDEEMTELMKVQRSYQASAKVMSTAVQLLDVVINGII"
     gene            3814..4746
                     /gene="flgL"
                     /locus_tag="KRL67_002739"
     CDS             3814..4746
                     /gene="flgL"
                     /locus_tag="KRL67_002739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgL"
                     /protein_id="HBH1523605.1"
                     /translation="MRVSTGMMSSSYLNSLQDNLQRLDKVNRQINTTKEINKLSDNPY
                     KAIKILNSKSEIKTMETYIENCKDTADWLETTDTSLDQLGNLLADIKKGLVSSGNGSY
                     SDDEIKTISNSTNEKMKEIANALNATHEGKYIFSGSNTGTPPVECVENADGSVSLKFN
                     ASLNLNKLNDSLSVDVAQGISVDYNVKLSSLGFDPTKTDPFEKLNNISKKLANPSDAN
                     IKELTTTCLGDMENLIDNTVNVRSIYGTKANTVDAMKEKNDEGLIQLKDVLSQNDEID
                     YGEKLVQLKAAELTYQASLQTGGKLFNVSILDYI"
     gene            4762..5154
                     /locus_tag="KRL67_002740"
     CDS             4762..5154
                     /locus_tag="KRL67_002740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly protein FliW"
                     /protein_id="HBH1523606.1"
                     /translation="MMKITLKKGILGFENLKEYELLDIENEDVLKEFNSTEEDCIGFI
                     VVSPFEIIKEYEIVLNQETIEKLEVKSPNDIMLLNIITVGQTLEESTVNMKAPIVINV
                     RNNCGMQIILQDEKYSIWHPLLRGDGGC"
     gene            5148..5360
                     /gene="csrA"
                     /locus_tag="KRL67_002741"
     CDS             5148..5360
                     /gene="csrA"
                     /locus_tag="KRL67_002741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011987028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon storage regulator CsrA"
                     /protein_id="HBH1523607.1"
                     /translation="MLVISRKKDEAVLIGDNIEVKVVGVDGNNIKLAISAPNNISILR
                     KEIYEKVKNENIKATNKNIKILKSLK"
     gene            5382..5777
                     /gene="fliS"
                     /locus_tag="KRL67_002742"
     CDS             5382..5777
                     /gene="fliS"
                     /locus_tag="KRL67_002742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FliS"
                     /protein_id="HBH1523608.1"
                     /translation="MYGENPYNSYKQNAIFMASKEQLLLMLVDGAVKYTKIARGAIID
                     KNTRKAHRELIRVQDIFTELMVTLDQNAGQWAKDMYRVYDFVRYELSRANIRKDIQII
                     DNVLPVIEEIKDTWHEADRKSREERSRYK"
     gene            5777..6127
                     /gene="fliS"
                     /locus_tag="KRL67_002743"
     CDS             5777..6127
                     /gene="fliS"
                     /locus_tag="KRL67_002743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FliS"
                     /protein_id="HBH1523609.1"
                     /translation="MELDLNELNGLSKEELLLMLVDGTVKYINISKVALLNRDYLKAH
                     NELVRVQNIFTELMVTLDQSVGQWAKDMYKVYEFIKHELVKADINKDIKIIDDILPIA
                     EQIRDTWHEVYNKL"
     gene            6155..7678
                     /gene="fliD"
                     /locus_tag="KRL67_002744"
     CDS             6155..7678
                     /gene="fliD"
                     /locus_tag="KRL67_002744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar filament capping protein FliD"
                     /protein_id="HBH1523610.1"
                     /translation="MSSISPVRVTGLSGNFDMEGIIEASMIRDKEKVNKAKQDQQIVK
                     WKQEIYRDIIKESKNLYDKYLNGDSPNSITNKKAYSATRITSSDESIIVAKGSAGAEK
                     INYQFAVSQMAEPAKVTIKLNSSDPIVQQFPPNASGASSLNIGGVNIPISEQDTTSTI
                     VSKINSLCADNDIRASYSEMTGELIISRKQTGSSSEIDLKVIGNDSLAGQIASDNGIT
                     FTTDASGTKSAVVYGKNLEADVTDDQGRVTHISKEQNSFKIDNIDYNVNSKGSAKLVS
                     VTDTEEATKNMKAFVDDYNALMDKVYGLVTTKKSKDYPPLTDEQKEDMTTEEIEKWEK
                     KAKEGILRNDDELRAFVEDIQSMFFGDADTIIALRKLGISEHENYNKKGQISFNADTF
                     SKALIDDSDKVYKALAGYSSNYDDKGMFEKLKKIVFEYSGSSASKFAKKAGIEKTSSA
                     SQNVYSKQIAEQERNINSLVEKMNDKEKRLYAKYSALESLLNQYSSQMNYFSQAQGN"
     gene            7681..8016
                     /locus_tag="KRL67_002745"
     CDS             7681..8016
                     /locus_tag="KRL67_002745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FliT"
                     /protein_id="HBH1523611.1"
                     /translation="MDKLEEKINLYKDISLKIINFIEKMEYKNISFQLDERQNIINSI
                     SEVDKSEFIQLYDSMELFEIDAKIRDALQEQLSEVKKELHEYKLTKQVNTMYYSLNRE
                     KVNIFNKKV"
     gene            8117..8989
                     /gene="fliC"
                     /locus_tag="KRL67_002746"
     CDS             8117..8989
                     /gene="fliC"
                     /locus_tag="KRL67_002746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin FliC"
                     /protein_id="HBH1523612.1"
                     /translation="MRVNTNVSALVANNQMGRNVNGQSKSMEKLSSGVRIKRAADDAA
                     GLAISEKMRAQIKGLDQAGRNVQDGISVVQTAEGSLEETGNILLRMRTLSLQSANEIN
                     NTEEREKIADELAQLKDEIERISSSTEFNGKKLLDGTSTTIRLQVGASYGTNVSGTSN
                     NNNEIKIQLVNTASIMASAGITTTSIGSMKAGGTSGTDAAKTMVSSLDVALKSLNSSR
                     AKLGAQQNRLESTQNNLNNTLENVTAAESRIRDTDVASEMVNLSKMNILVQASQSMLA
                     QANQQPQGVLQLLG"
     gene            9083..11224
                     /locus_tag="KRL67_002747"
     CDS             9083..11224
                     /locus_tag="KRL67_002747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HBH1523613.1"
                     /translation="MLLSIVMMVKNEENILEKTLKSLSALRNSIESELIIVDTGSTDN
                     TIRIAKQYTEKVYFHNWNDDFSSMRNISISYAKGEWLLILDADEVLIDYSNIIKFFES
                     GLNKKFNSASVRLKNLYSFDKKLYGYCSVLRFFKNLDFKYCGKVHEQPMYKNPIFNNI
                     ADFEHYGYLFEDEEIRINKVKRNEKLLLEELKEDEKSPYTNYQIGKNLIILGKYQEAL
                     DYLEKSYKLYSKLEIVPGYLITNLAKVYLYLGKAKKCEKLCLKYINKDRNNIDIYYHI
                     AQAQVDLGKYENSIDSYKRYIYLLDNYEMSTQANSLLSDTDAVGFRDEAIITLIKIYY
                     KLEKYDLVISEYDNIEDVEKKKNVYFSLFMSLYKLNRFEDIKNYYREIPNSKVERDSF
                     YKSMEDFINNIKDDEKELIYKSLCDIEGNYGLLNKIRINKHVSLDTCRKILNEEKDIV
                     YASVIKIAFEEKVDLLDIFYNMEYVWIEKYLRYILALERSFNVSIYKYIISCPNTSEI
                     EKIRVYKVISKVLLESANLSDKKYKELFYIYIMYSYKYIKYIYSNLNDCELIKFVCND
                     IDKFTLEFKHLLDGKRKINNPNEKLEYLYNMRILLNEYPFYNRIIKIFISELEESITE
                     SKEFKLLKDNFIIRIENMIENGKINDAKLLIEDYSKTFADDVKILNIKAILFMCEDKF
                     EEADFALKKALSLDLENEDTIYNIEYLKNLK"
BASE COUNT         4834 a         1114 c         1986 g         3355 t
ORIGIN      
        1 aaatattttt tttatttatc cgataatcat aatgtagtga aactaaatct aaagttttaa
       61 taaatataaa agaaattaat ttaacaaagt agtctataac aagaagattt tataggaact
      121 caagtatctt gaattatata aggataactt taaaatttag aaaggggagt tttattttga
      181 acgaaataag ttcactaata aatatactgg cattgaccca attatctggt agtaatacca
      241 cgaatcaatg caactgtgga tataataatt cgtctggatt tgatatgatt atgatgacat
      301 tgttgaaagc attatcacaa aataatcaac aaacctctaa tggatataat ttatcatcta
      361 attctgaaaa actgtttaat gaacttgata gtgtagcaaa taatacagtt ggtaagttta
      421 ttgaagttga tacaaaagat aaaagtgtaa agtcgagaat tgaaaatgct gtagagcaag
      481 catcaaagaa atataatgta gatgcaaatc ttataaaggc aataattaag gttgaatcag
      541 attttaaccc aaatactgta tcttctgctg gtgccaaggg gcttatgcaa cttatgccag
      601 aaaattgcag agatttggga gtaacaaacc cttttaacat agaacaaaat atagatgctg
      661 gtactagaca tattaaggaa tatatagata tgtttggagg tagcatagaa atgggattga
      721 tggcatataa tggtggacct ggtagaatga gaagtagagg cgtagaatca atttctgact
      781 tgtataaaat gccaaaggag acacaaaatt atatacctaa ggttatgaaa tattatagag
      841 ggtaggattt actatgggta taaatataga caagctaaat ataagtccaa gtagaagtat
      901 agaagttaaa aagacaaagg aaaataataa gttttctgac agcttaaact ttgtaaaaca
      961 atcttcttca ctagaagata taaaaaggaa tttagatggt gtaaaaaagg ctggggagag
     1021 gcttgtactt acacaaaatt atggtgacat tagtaggtat aaaaatgcag taaaagaata
     1081 cctaaaatct gttgtagata atatgtattc acttgataaa agtagtagtt tctgggagca
     1141 tcaatattat aagaatgtaa agattattga taacaagctt gaggatatga ctaataaact
     1201 acttagtgaa gaaaaagaaa atatagatat tgtttctact gttgatatga tacaaggctt
     1261 attgatagat atgtatagat aagattacat aaggagaaaa gttttatgaa tagcaaaaaa
     1321 gaagatatat atgaattgaa ttttggtcaa ctaatagaag aaaagactgg gattatagag
     1381 gaaaatcaaa atgtttttaa tgcaaaagta aatgtggctg tttctatagg aaacataaca
     1441 gaaacaataa aaaatatatt agatttaaaa gaaggttcta taataaaact aaataagaat
     1501 gtagatgaaa aattagatat atatgcaaat gacaggttgt ttgcttatgg agaaagtatt
     1561 attacaaatg aaacattatc tgtaagatta tctaaaatag aagattcaga cataaattaa
     1621 tactttaaaa aatatcccct tagattattc tatattggtt taatcaaggg gtatttttat
     1681 aaaaaattta aaatctattt gaatcgtata aatatttttc ttatttgagc gataattaaa
     1741 atataaggat gtaattttgg aggtgaggtt atgaatatta aaagtgtaag ttcaaatatt
     1801 gtagtaaata attatctaaa gaataaatcg gatgtaaaaa aggtacaaca agttcaaaaa
     1861 gttgataaaa cagacaaaat agaactatct aaatttgcaa aagaaatgtc aagtgtatca
     1921 aatatgagcg atgaagcgac tagaaataaa aaaatagaag aaataaaaag tgcattagaa
     1981 aatggaactt acaaaattaa accagaagaa attgctaaaa aaatgataga ggagatgcga
     2041 ggataaagtc atgattacag atttaaaagt cataatatat gaagaaaaaa agataattag
     2101 taatatgatg gaattactaa aagaacaatt tgattatata attgaaaaag acttagaaaa
     2161 tttaaatagg ttgaatccta aactagagga tattagtaga gaacttgcat ctatagaaat
     2221 aaaaagaaga caaattttag gggatgatgt ttcaataagt gaggttgttg aaaacagtaa
     2281 tgatgaatat ttaaaagaaa tatatataga cttaaagcat attcttgctt tagctaaaaa
     2341 tcaacaagaa tcaaatgatt ctttaataaa gaaagaactt attttcacga aaaaaatgat
     2401 taatttcata aaaccagttg ataaacaaac tacaacttat aattcttatg gaaacattag
     2461 aaaatagata ttgggaggag aaatatggca ggattatttt ccattttaaa tacagctaat
     2521 tcaggaatga gtgcgcacca aaaatcaata cagacaattt ctcataatat aagtaaccta
     2581 gatacagatg gttattctag gcaaaggacc gagtttgcta caaatagtcc aatgtatatg
     2641 cctagtttga gcaattcaat tggaagggga caattaggta ctggagtaca tgtaacagat
     2701 gtaacaagag ctagaaatag tttttatgat taccagttta gagcagaagc tcataagtat
     2761 gggaatacag tttcaaaata tgattattat aatactatag aaactatact aaatgaacca
     2821 tcagactatg gtatatctgc aggtatagat gattttttta aaggatggga gtcattatca
     2881 aaggatataa atagtgatag taaaaaaagt ctggtggtag aaaatgcatc taatttagcg
     2941 actttaataa gcgaaagtta taagaagctt gataagttaa aaggagatat agattccaat
     3001 atagatactg agataaaaca aataaatgac atgttaaaaa gtcttgaagg cttaaataaa
     3061 tctattgata ttatctctgg ttctggctct acaccaaatg atttgcttga tgaaagagat
     3121 agaatattgg ataacttgag ttttaagtta gaccttgaaa atagcgatgt taaaaatatg
     3181 ctttcagatg gtaaattaga aattaatgag ttgaaaaatg tagatggaac ttggaaaaca
     3241 ggtatatcag gaactttaca aggcttgttt gaaatgcatg gtaaaattga tacatataaa
     3301 agcgacttaa aagatgtatc tgatggatta gcaaaacaga taaatgatgt ttataataca
     3361 tctgctggaa taactgtaag agattttttt ataacctcaa atgtagctgg tgaagatatt
     3421 ataaaagtaa atccagcaat aaaaagtaat tcaaatgaat tgaagcttac tactgaagaa
     3481 gcttcaaaga ttgctaagtt aaaggatgaa aaaatagata ttggggtagc tggtggaaaa
     3541 gtaagcacaa taagtgacca ctacaaggct tttgcagaat cggtaggttt agattctcaa
     3601 aaagttaatc aagatgaagt gaatcaacgt aaaattataa ataatgttga taattcacga
     3661 atgagtgttt caggtgtatc tttagatgaa gaaatgacag aactcatgaa agtacaacgt
     3721 tcctatcaag ctagtgcaaa agttatgtca acagcagtac aactattgga tgttgtaata
     3781 aatgggataa tttaataaaa ggaggaattt ataatgagag ttagtacagg tatgatgtcc
     3841 agtagttatc taaattcatt gcaagataat ttgcaaagat tagacaaggt caatagacaa
     3901 atcaacacaa cgaaagaaat aaataaacta tcagataatc catataaggc aataaagata
     3961 ttgaattcaa aaagtgaaat aaaaactatg gaaacttata ttgaaaattg taaagataca
     4021 gcagattggt tagaaacaac tgatacttca ttagaccaac ttggtaattt attagcagat
     4081 ataaagaaag gtttggtatc aagtgggaat ggttcatatt cagatgacga aataaaaact
     4141 attagtaatt caaccaatga aaaaatgaaa gaaatagcaa atgctttaaa tgcaacacat
     4201 gaaggtaagt acatattttc aggttcaaat acaggcacac cacctgttga gtgtgtagag
     4261 aatgctgatg gctcagtaag tttaaagttt aatgctagct taaatcttaa taaattaaat
     4321 gattctttaa gtgtagatgt agctcaagga atcagtgtag attataatgt taagttgagt
     4381 agtcttgggt ttgatccaac aaaaactgat ccatttgaaa agttaaataa tatatcaaag
     4441 aaactggcaa atccaagtga tgcaaatata aaagagttga caacaacttg tttaggtgac
     4501 atggagaatc taatagataa tacagtaaat gttaggtcta tatatggtac aaaggcaaac
     4561 acggtagatg ccatgaaaga aaaaaatgat gaaggtttga tacagttaaa ggatgtttta
     4621 tcgcaaaatg atgaaataga ttatggggaa aagcttgttc aattaaaggc tgcagaatta
     4681 acttatcaag cttcactaca aacaggagga aagttattta atgtgtctat attagattat
     4741 atatagtaaa ataacgcaga tatgatgaag attacattaa aaaaaggaat actaggattt
     4801 gaaaacttaa aagagtatga gcttttagat atagaaaatg aagatgtttt aaaagaattt
     4861 aattctactg aagaagattg tataggattt attgttgtat caccatttga aattattaag
     4921 gaatatgaaa tagtcctaaa tcaagaaaca atagaaaaac ttgaagtaaa atctccaaat
     4981 gatattatgt tgcttaatat aatcactgtt ggtcaaacac ttgaagaatc aactgtgaac
     5041 atgaaagctc ctatagtaat aaatgtaaga aataattgcg gaatgcaaat aattttacag
     5101 gatgaaaaat attcaatatg gcatccatta ttgagaggtg atggtggatg ctagtaattt
     5161 cgagaaaaaa agatgaagca gtacttatag gagataacat agaagtaaaa gttgtaggag
     5221 tggatggaaa taacataaaa ttagccatat ctgctcccaa taatataagt atcttaagaa
     5281 aagaaatata tgaaaaagta aaaaatgaaa atataaaagc cacaaataaa aatataaaaa
     5341 tcttaaaatc attaaaataa aacacttgga ggaagtaata tatgtacgga gaaaatcctt
     5401 ataattctta taagcaaaat gctattttta tggcatcaaa agaacaatta ttgcttatgc
     5461 ttgttgatgg tgcagtaaaa tatacaaaaa tagctagagg agctattata gataaaaata
     5521 cacgaaaagc acatagagag cttataagag ttcaagatat atttacagaa ctaatggtta
     5581 cattagacca aaatgcaggt caatgggcga aagatatgta tagagtttat gattttgtaa
     5641 gatatgaact ttcaagagca aatatcagaa aagatataca aataatagat aatgtattac
     5701 ctgtaataga agaaattaaa gatacatggc atgaggctga tagaaaaagt agagaagaaa
     5761 gaagtaggta taaatgatgg agttagattt aaatgaacta aatgggttat caaaagaaga
     5821 attactattg atgcttgtag atggtacagt caagtatatt aatatatcta aagtcgcact
     5881 tttaaataga gactatttaa aagcacacaa tgaacttgtt agagttcaaa atatatttac
     5941 agaattaatg gtcacattag accaaagtgt aggacaatgg gcaaaggata tgtataaagt
     6001 ttatgaattc ataaagcatg aattagtaaa ggctgatata aataaagata ttaaaataat
     6061 tgatgatata ttaccaatag cagaacaaat aagagataca tggcatgaag tatataataa
     6121 actataacta taatcatgat ggggggataa acatatgtca agtataagtc cagtaagagt
     6181 tacaggcctt tcaggaaatt ttgatatgga aggtataata gaagctagta tgataagaga
     6241 taaggaaaaa gttaataaag caaaacaaga tcaacaaata gttaaatgga aacaagaaat
     6301 atacagggat attataaaag agtcaaagaa tctttatgac aagtatctaa atggagactc
     6361 tcctaatagt ataacaaata aaaaagcata ttctgctaca agaataacta gttctgatga
     6421 aagtattata gtagcaaaag gctcagctgg tgcagaaaaa ataaattatc aatttgcagt
     6481 ttctcaaatg gctgaaccag caaaagtaac tattaagtta aattcaagtg accctattgt
     6541 tcaacagttt cctccaaatg ctagtggagc aagttcttta aatataggtg gtgtaaatat
     6601 accaatatct gaacaagata ctacaagtac tattgtaagt aagataaact ccctttgtgc
     6661 agataatgat ataagagctt cttatagcga gatgacaggt gaattgatta tatcaagaaa
     6721 acagactggt tcatcatcag agattgattt aaaagtaatt ggaaatgata gtttagctgg
     6781 acaaattgcc agtgataatg gtattacatt tacaactgat gctagtggaa ctaaatctgc
     6841 agtagtatat ggaaaaaatt tagaagctga tgtaactgat gaccaaggaa gagtaacaca
     6901 cataagtaaa gaacagaatt catttaaaat agataacatt gattataatg taaattcaaa
     6961 aggaagtgct aaactggttt ctgttactga cactgaagaa gctactaaaa atatgaaagc
     7021 atttgtagat gattataatg cattgatgga taaggtttat ggattagtta ctactaaaaa
     7081 atcaaaagat tatccacctc ttacagatga acaaaaagag gatatgacaa ctgaagaaat
     7141 agaaaagtgg gaaaagaagg caaaggaagg aatacttaga aatgatgatg aattaagagc
     7201 ttttgttgaa gatattcagt ctatgttttt tggagatgca gatactatta ttgctttaag
     7261 aaaactgggt attagtgagc atgagaatta taacaaaaaa ggacaaatat catttaatgc
     7321 agatactttt tctaaggctc ttatagatga tagtgacaag gtatataaag ctttagctgg
     7381 atattcttca aattatgatg ataaaggaat gtttgaaaaa ttaaaaaaaa ttgtatttga
     7441 atattctgga agttcagctt ctaaatttgc taaaaaagcg ggtatagaaa agacttcttc
     7501 tgctagtcaa aacgtatatt caaaacaaat tgcagagcaa gaaagaaata taaatagttt
     7561 agttgaaaaa atgaatgata aagagaaaag actttatgct aaatattcag ctttagagtc
     7621 tttgttaaat caatattctt cacaaatgaa ctatttctca caagcacaag gtaattaatc
     7681 atggacaagt tagaagaaaa aataaattta tataaagata tatctttaaa aattataaat
     7741 tttattgaaa aaatggagta taaaaatata agttttcaat tagatgagag acaaaacatt
     7801 ataaatagta taagtgaagt tgataaaagt gaatttatac aactttatga tagtatggaa
     7861 ctatttgaaa ttgatgctaa gataagagat gctttgcaag aacaattatc agaagtaaaa
     7921 aaagaattac atgagtacaa gcttaccaaa caggtaaaca caatgtatta tagtttaaat
     7981 agagaaaaag taaatatttt taataaaaaa gtataaagtt atagattaac ttgtccgata
     8041 atataaatgg atagttaata caaaagttaa aagtagaaat gacaaggatg tcaactatac
     8101 taaggagggt aaaataatga gagttaatac aaatgtaagt gctttggtag caaacaatca
     8161 aatgggaaga aacgtaaatg gacaaagtaa gtctatggag aagttatctt ctggggttag
     8221 aatcaagaga gctgctgatg atgctgctgg acttgcaata tctgagaaaa tgagagctca
     8281 aataaaaggt ttagaccaag caggaagaaa cgttcaagat ggtatatctg ttgtacaaac
     8341 agcagaaggt tcgttagaag aaactggaaa tatactacta agaatgagaa ctttatcatt
     8401 acaatctgca aatgaaatta ataatactga agaaagagaa aagatagctg atgagttagc
     8461 acaattaaaa gatgagattg aaagaatatc aagttcaaca gagtttaacg gtaagaagct
     8521 acttgatgga acttctacaa caataagatt acaagttgga gcaagttatg gaactaatgt
     8581 ttcaggtaca agtaataata acaatgagat aaaaattcaa ttagtaaata ctgcaagtat
     8641 aatggcttca gctggtataa caactacttc aataggaagt atgaaggcag gtggaacttc
     8701 aggaacagat gcagctaaaa ctatggtatc tagcttagat gtagctctta agagcttaaa
     8761 ctcatcaaga gcaaaattag gagcacaaca aaacagacta gaatcaactc aaaacaattt
     8821 aaataatact ttagagaatg ttacagcagc agaatcaaga ataagagata cagatgttgc
     8881 ttcagaaatg gttaacttat ctaaaatgaa tatattagtt caagcatcac aatcaatgct
     8941 tgctcaagct aatcaacaac cacaaggagt tttacaatta ttaggataga ataaatttag
     9001 tatattgaat aaaaaagaaa ggataaggct ttgctttatt cttttctttt tttagaaata
     9061 tagttttgaa aggaaaatat gtatgttact tagtatagtt atgatggtca aaaacgaaga
     9121 aaatatactt gaaaaaactt taaaatcgtt atcagcattg agaaattcaa tagagagtga
     9181 acttataata gttgatacag gttctacaga taatactata agaatagcaa aacaatatac
     9241 agaaaaggta tattttcata attggaatga tgatttttct agtatgagaa atatatctat
     9301 atcctacgct aagggcgaat ggttacttat attagatgca gatgaagtat taattgatta
     9361 ttcaaatatt ataaagtttt ttgaaagtgg tcttaataaa aaatttaact ctgcctcagt
     9421 acgactaaaa aatctttatt catttgataa aaaattatat ggatattgtt cagtgttgag
     9481 atttttcaaa aatcttgatt ttaaatactg tgggaaagtt catgaacaac caatgtataa
     9541 aaatcctata tttaataata ttgctgattt tgaacattat ggatacttgt ttgaagatga
     9601 agagataaga ataaataaag taaaaagaaa tgaaaaactg ttgcttgagg aattaaaaga
     9661 agacgagaaa tctccatata caaattatca aatagggaaa aatcttatta tattaggaaa
     9721 atatcaagaa gctttagatt atctagaaaa gtcttataaa ttatactcaa aattagaaat
     9781 agtacctgga tacttgataa caaaccttgc aaaagtatat ttatacttag gaaaggctaa
     9841 aaagtgtgaa aaattatgct taaaatatat taataaggac agaaataata ttgatatata
     9901 ttatcatatt gcacaagcac aagttgattt agggaagtat gaaaatagta tagattcata
     9961 taaaagatat atttatttgc tagataatta tgaaatgagt acgcaagcaa atagtttgct
    10021 ttcagatact gatgctgtag gatttagaga cgaagctata attacattga taaaaattta
    10081 ttataagctt gagaaatatg atttagtaat ttcagagtat gataatattg aagatgtgga
    10141 aaaaaagaag aatgtgtact ttagcttatt tatgtcctta tataaattaa atagatttga
    10201 agacataaaa aactattata gagagattcc aaattcgaag gttgagagag atagttttta
    10261 taaaagtatg gaagacttta taaataatat aaaagatgat gagaaagagc tcatatataa
    10321 aagtctttgt gatattgaag gtaattatgg tcttttgaat aaaataagaa taaacaaaca
    10381 tgtatcatta gatacatgta gaaaaatact aaatgaagaa aaagacatag tttatgcaag
    10441 tgttataaaa atagcttttg aggaaaaagt tgacttatta gatatttttt ataatatgga
    10501 atatgtttgg atagagaaat acttaaggta tattttagca ttagaaagaa gttttaatgt
    10561 atcaatatat aaatatataa ttagttgtcc taatacatca gaaatagaaa agataagagt
    10621 gtacaaagtt atatctaaag ttcttttaga aagtgctaat ttaagtgata aaaaatacaa
    10681 agaacttttt tatatttata taatgtactc ttataaatac ataaaataca tatatagcaa
    10741 tttgaatgat tgtgaattaa taaagtttgt ttgtaatgat atagataaat tcactttaga
    10801 atttaagcat ctacttgatg gtaaaaggaa gataaataat cctaatgaaa aattagaata
    10861 tctatataat atgagaatat tattaaatga ataccctttt tacaatagaa taataaaaat
    10921 ctttataagt gaattagaag aaagtataac agaaagtaaa gaatttaaat tattaaaaga
    10981 taactttatt attcgtatag aaaatatgat tgagaatgga aaaataaatg atgcaaagtt
    11041 attgattgaa gactactcaa aaacatttgc agatgatgta aagattttaa atataaaggc
    11101 aattttgttt atgtgtgaag ataaatttga agaagctgat tttgcattaa aaaaggcatt
    11161 atcactagac ttagaaaatg aggatactat atataatata gagtacttaa aaaatttaaa
    11221 ataaattcaa aactaggtta tttagaaaat aataatataa ataacagtta atgaaaaatc
    11281 taatagaag
//
LOCUS       DAEPXF010000124        11169 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain HB27
            SAMN13376389-rid12479423.denovo.131, whole genome shotgun sequence.
ACCESSION   DAEPXF010000124 DAEPXF010000000
VERSION     DAEPXF010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13376389
            Sequence Read Archive: SRR10518432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 11169)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11169)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001069624.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001069624.1
            Genome Coverage       :: 315x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/15/2021 01:54:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,165
            CDSs (total)                      :: 4,076
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 89
            rRNAs                             :: 10, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 10 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 19 of 69
            Pseudo Genes (incomplete)         :: 44 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 10 of 69
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11169
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13376389-rid12479423.denovo.131"
                     /strain="HB27"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="China: Hebei"
                     /collection_date="2017"
     gene            complement(15..842)
                     /locus_tag="KRL67_002748"
     CDS             complement(15..842)
                     /locus_tag="KRL67_002748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HBH1523614.1"
                     /translation="MKIKDNKAVFHVFIRIFLIAVLILTGTLLEEKINNVPVENISSK
                     GENTLELLSQSNTLGEIIYKASQEETKGPGATSEKVAYITIDDGPSKYTNALLDILKE
                     NDVKATFFMLNQNMKNHKEEVRRVLEEENSIGFHSVSHDIHKLYKSPEVTVGEFNTCN
                     NTLQEITGQSSKLIRLPYGSKPYMPEGSYNKLMANDYLIWDWNLDTQDWKSSTSQIVS
                     NILYYGRKRDELVVLIHEKEQSLNALNNIIRILKERGYTILPITENITPKNFWQENL"
     gene            complement(890..2326)
                     /locus_tag="KRL67_002749"
     CDS             complement(890..2326)
                     /locus_tag="KRL67_002749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron hydrogenase"
                     /protein_id="HBH1523615.1"
                     /translation="MNTRNDNSKILEIYKMKLFGELVKAVWNDDIEDIENLPNKILNE
                     DLKDNLNNYFSGKFDKDTMVNLIRVTMGLDVTEDTDKNLKESLHEAVNLDEVKRPIIS
                     VISDACKYCDENNKEDESCKIRSKHINCNEEDVCSACGDCVSRCKLGAISDKIEFIPM
                     IKLLKDIKSPVYAIVAPAFVGQFGKNVSPGKLRTALKKIGFEDMIEVALAADMLTAKE
                     AYDYYEHMKKDEEGCFITSCCCPIWVSLIQNSFPQILENVSPSVSPMIACGRAVKILN
                     PDAKVVFIGPCMAKKKEAMLEDIKDAVDFVLTFKELQEIFDALELNPAIMDESDRVEA
                     SLSGRVYARTGGVSKAVKLSVRRIDKNIKFEEKAFEGTKECMDGLKKVLNKEIEATFI
                     EGMGCVGGCVGGPKRILSVEEGTKNVDEYSESTNMETPFDNLNVLQFLTSLGIKRVES
                     LGEKEEEKVKKIFSRDIKSNKKENKYKK"
     gene            complement(2413..3282)
                     /locus_tag="KRL67_002750"
     CDS             complement(2413..3282)
                     /locus_tag="KRL67_002750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HBH1523616.1"
                     /translation="MKLQKMGMRTIKTGISVTLCMILGDYLVENMFYSLVACIISVQD
                     TVKGSLKTGINRVEGTILGGIIGFIFAIIKAGDPFLCGLGVMCSIYLCNLFKIKSVVV
                     ACVTFLAIHLGVGNSDPVYYSLHRVLDTSVGVLFGVLVNYYVARPNYLNSVIDEFKKI
                     EELSMLLIEDKILRKENLNVEKFEEEVEKLEEIYSKLIDELDYSLSETNIEKIENALR
                     ICREIHFHMQSIELLENKLYLKEHTYKYLKKTYNVDKLDWNIDEEKSPVFNYHLMKIM
                     IEIELLRKLNKSY"
     gene            complement(3441..4094)
                     /gene="phoU"
                     /locus_tag="KRL67_002751"
     CDS             complement(3441..4094)
                     /gene="phoU"
                     /locus_tag="KRL67_002751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HBH1523617.1"
                     /translation="MVNNSLDISIDTLKEQTVLMMEKCEEAIEKSVTCMIEKDLEGAR
                     KVVEDDEEINKLREEIRDKSIELIVLKQPMARDLRFIYSLGNISIELERIGDYAANIA
                     REMLKIGGEAYIKELIDIPKMAKECKSMMSQTREALILFDASIAYDVGTKDDIVDGLY
                     EQVQIDCLKIMHENSNTIDQGVKLLLIGRYLERIGDHITNVCEKIIFAIEGKMVEIG"
     gene            complement(4122..4886)
                     /gene="pstB"
                     /locus_tag="KRL67_002752"
     CDS             complement(4122..4886)
                     /gene="pstB"
                     /locus_tag="KRL67_002752"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HBH1523618.1"
                     /translation="MELIDKIKMSVKDLDLFYGDKQALKKINMDIKENKVTALIGPSG
                     CGKSTFIRTLNRMNDLIEDVTIKGNISVDGEDIYTSDDVINLRTKVGMVFQKPNPFPM
                     SIYDNVAYGPRTHGLRDKKQLDKIVEESLKGAAIWDEVKDRLKSSALGLSGGQQQRIC
                     IARAIAMRPEVILMDEPTSALDPISTLKVEELIEDLKKDYTIVIVTHNMQQAARISDE
                     TAFFLNGEVIEFSDTKTMFTTPVDKRTEDYITGRFG"
     gene            complement(4905..5729)
                     /gene="pstA"
                     /locus_tag="KRL67_002753"
     CDS             complement(4905..5729)
                     /gene="pstA"
                     /locus_tag="KRL67_002753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003460575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HBH1523619.1"
                     /translation="MRKFKENLLKSLVYLSALFTIVSLVVIVGFIFIKGIGNMNFNFL
                     FSNYSASGDGGILPMIITTLYTVVVSIVVATPIGILAAIYLQEYAKKGKAVTAIRFAT
                     ESLSGIPSIVYGLFGGIFFVVTLKMGYSIVAGSLTVAIIILPVIIRTTEEALKTVPQA
                     YRESSLALGATKFQTLYKVVLPSAIPGILSGVILSVGRIIGESAAILLTAGTVAKMPG
                     GIFDSARTLTVHSYLLTKESGDIATAASIGIVLIVIVLALNMLARFVAKKLNKANY"
     gene            complement(5731..6648)
                     /gene="pstC"
                     /locus_tag="KRL67_002754"
     CDS             complement(5731..6648)
                     /gene="pstC"
                     /locus_tag="KRL67_002754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HBH1523620.1"
                     /translation="MIVKSRLVGLEKNDNNGNKTKYVIEKVAKNIFLLSALVAVASLL
                     LIIGFVFYKGLRPFIFEGYSFIDFLTGADWIPSANKFGISSMIVASIFATLGALVIGV
                     PVGILTSVFIAEVAPKRLAKIMSPAVELLAGIPSVLYGVFGLAVIVPTIQSIFNLPKG
                     QSLLAIVIVLAVMMLPTIISVSETAIRAVPKAYKEGSLALGASKIETIFKVVLPAAKS
                     GVLAAVVLGVGRALGETMAVILVAGNSPVMPTVITDSVRPLTTNIALEMGYAFGTHQE
                     MLFATGVVLFTFILILNLVLNKLSNKAVN"
     gene            complement(7030..8265)
                     /locus_tag="KRL67_002755"
     CDS             complement(7030..8265)
                     /locus_tag="KRL67_002755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBH1523621.1"
                     /translation="MRIFYLEIKRVLHSRRTLILLLLALVLSALMAFLPITYEGINYL
                     DDNGKVVELSGLDAIKYKKTIYANNSEISSGVVTPKKLKHALNIWKKTEEKYGKVGSE
                     DFPLDVYSKSILPIRPLLNRLPEAFANSETGFATELSEINTNDLDSFYEQCKTHLNDI
                     MKIERQTSSAQMKAQELYRKVDTPFEIYPGYSKDAFDYIIFYIFILLIICVAIAAPTF
                     SGEYQTQSDSILRCTKHGHIRLAFTKILALFSIFIIMFVICISIHLAISDLAFGTECL
                     KTSFQMLFSVISLVNINLLQAQILIAIGGLISILAMVSLTLFLSAKCKDSLTSMLIAF
                     SICLIPMGIGVSWIGYLLPPGGIGFKISLLYQMTSFNFLHLGGMSFWTPHIILFFSVI
                     EIPIFIFLAIRTYCKHQVS"
     gene            complement(8266..9153)
                     /locus_tag="KRL67_002756"
     CDS             complement(8266..9153)
                     /locus_tag="KRL67_002756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBH1523622.1"
                     /translation="MELKIEHVTKKFKDKIAVEDVCLTLTPGVWGFLGANGAGKTTLM
                     RIITDIMKPTNGKILYNGKNIYTMGAEYRNLLGFLPQEFGFPQDFSVKDYLEYIAALK
                     DVPPREVKGKINNLLEILTLSDVKNKKIIKLSGGMKRRVGIAQAMLNDPKILIMDEPT
                     AGLDPGERVRFRNFISEFSHNRVVLISTHIVSDIEYISTQNAIMKSGKILDVGTTDKL
                     VKKVEGKVWECLISNRDISKYEMNLRIINQRSEKNNQVSIRYLAEKSQIDNSIAVEPR
                     LEDLYLWLFPEAVYSKEGK"
     gene            complement(9298..10413)
                     /locus_tag="KRL67_002757"
     CDS             complement(9298..10413)
                     /locus_tag="KRL67_002757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBH1523623.1"
                     /translation="MKKINNFTYRKLRDKYEFIKREKNTEAYLKYKLYIMLAIYTGIV
                     LVLMFMADYTFSNFSNPLVKWLKLRRDVLFFLVFIIGYITIFNFYWKKPWNYLREIIN
                     ATHTIYQQNDELVELSKPLKEIEQQMNQIKMSVLLSQQAVKEAENKKNNLVMYLAHDI
                     RTPLTSVIGYLSLLKEIPDMDNSQKSKYTEVALDKAQYLEKLINELFEITRYNSNQIK
                     INFEKVDLYYMLLQLKDEFYPLLSEKGNRAILKANESLTIKADSEKLARVFNNILKNA
                     IAYSYPNTEIIISAVHKDSKVDIAFRNYGQTIPEEQLSAIFEKFNRLDEARKSDMGGA
                     GLGLSIAKEIINLHGGDITVQSKDEVTTFTISLPTSI"
     gene            complement(10449..11147)
                     /gene="vanR"
                     /locus_tag="KRL67_002758"
     CDS             complement(10449..11147)
                     /gene="vanR"
                     /locus_tag="KRL67_002758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002594737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanR-ABDEGLN family response regulator
                     transcription factor"
                     /protein_id="HBH1523624.1"
                     /translation="MNGKILVVDDERDIADLIEVYLTNENYIVYKFYSGLDALNCINL
                     NEFDLAILDVMLPGVNGFSLCQKIREKYTYPIIMLTAKDEETDKITGLTLGADDYVTK
                     PFRPLELVARVKAQLRRYKKYSGSLQSGEELSHIFSFLGLELNTQTYECHLNGKALSL
                     TPTEFSILHVLLKNKGSVVNSENLFHAIWKDEYYNKSNNTITVHIRHLREKLGDTVDE
                     AKYIKTIWGVGYKI"
BASE COUNT         3584 a         2048 c         1252 g         4285 t
ORIGIN      
        1 catatttaaa tttattataa gttttcttgc cagaaatttt taggtgttat attttctgta
       61 attggcaata ttgtatatcc tctctctttt aagattctta taatattatt taaagcattt
      121 aaactttgct ctttctcatg aattaatact actaattcat ctctttttct tccataatat
      181 aatatattac ttactatctg acttgtagaa gacttccaat cctgtgtatc caaattccag
      241 tcccaaatta aataatcatt tgccataagt ttattatatg aaccttctgg catatatggt
      301 ttacttccgt atggtaatct tattaatttt gaactctgac cagttatttc ttgtagagta
      361 ttattgcatg tattaaattc cccaactgtt acttctgggc ttttatataa cttatgtata
      421 tcatgactta cactatgaaa acctattgag ttttcctctt ccaagactct ccttacttct
      481 tccttgtggt ttttcatatt ttgattaagc atgaaaaatg tcgctttgac atcattttct
      541 tttaaaatat caagtagtgc atttgtatat tttgatggac catcatctat tgttatgtat
      601 gctacttttt cagaagtagc ccctggacct tttgtttctt cttgagatgc tttgtatatg
      661 atttctccta aagtattact ttgagaaagc agttccagtg tattttcacc ttttgaactt
      721 atattttcaa ctggaacatt attaattttt tcctccaata atgttccagt taaaattaaa
      781 acagctataa gaaatatcct tataaataca tgaaaaacag ccttattatc tttaattttc
      841 atcccccata tctccctttt ataaaagcat aagtttaaga cttaaaaagt tattttttat
      901 atttattctc tttcttatta ctttttatat ctctactaaa tattttttta accttttctt
      961 cttctttctc tcctaaagat tctactcttt tgattcccaa agaagttaaa aattgtaata
     1021 catttaaatt atcaaatgga gtttccatat tcgtactttc actatattca tcaacattct
     1081 tagtaccttc ttccacactt aaaatacgct taggacctcc tacacagcct cctacacacc
     1141 ccattccttc tataaatgta gcttctattt ctttattcag aactttcttt agaccgtcca
     1201 tgcactcttt tgttccttca aatgcctttt cttcaaattt tatatttttg tcaattcttc
     1261 taactgataa cttaactgct ttacttacac ctcctgttct agcataaact ctacctgaaa
     1321 gggacgcctc tactctatca ctctcatcca ttattgctgg atttaactct aatgcatcaa
     1381 atatttcttg caactcttta aatgtaagca caaaatcaac tgcatctttt atatcttcta
     1441 acatcgcttc tttctttttt gccatacaag gacctatgaa tacaacttta gcatctggat
     1501 ttaaaatttt tacagctctc ccacaagcaa tcataggaga aacggatggc gatacattct
     1561 ccaatatctg aggaaaacta ttttgtatca aactaaccca aataggacag caacaactag
     1621 ttataaagca tccttcttcg tcctttttca tatgctcata ataatcatat gcttcttttg
     1681 cagtaagcat atctgctgcc aaagcaactt ctatcatatc ttcaaatcct atctttttta
     1741 atgcagttct taatttacca gggcttacat ttttcccaaa ctgtcctaca aaagctggtg
     1801 caactattgc atatacaggg ctctttatat cttttaacaa ttttatcatt ggtataaatt
     1861 ctattttatc tgaaattgca cccaacttac atcttgatac acaatcacca caagcactac
     1921 atacatcttc ctcattacaa ttaatatgct tacttcttat cttacatgac tcatcttcct
     1981 tattattttc atcacaatat ttgcaagcat ctgatattac agatattatt ggtcttttga
     2041 cttcatctaa atttacagct tcatgtagtg actcttttaa atttttatct gtatcttctg
     2101 tcacatccaa tcccatagtt accctaatta aatttaccat agtatcttta tcaaattttc
     2161 cgctaaaata attatttaaa ttatccttta aatcttcatt taaaatttta ttaggtaagt
     2221 tttcaatatc ctctatatca tcattccaca ctgcttttac aagttcgcca aaaagcttca
     2281 ttttatatat ttctagtatt ttagaattgt catttctagt attcataaaa aatagtacct
     2341 ccataaatta aacttatata tgtttaatat ttacaaagat actatttttt atttattatt
     2401 taactactat tattaatatg atttatttag ctttctcaac aactctattt caatcattat
     2461 tttcattaga tgatagttga acacagggct tttttcttca tctatattcc agtctagctt
     2521 atccacatta tatgttttct taaggtattt atatgtatgc tctttcaaat aaagtttatt
     2581 ttcaagtaac tctatagact gcatatgaaa atgtatttct ctacatatcc ttaaggcatt
     2641 ctcaattttt tctatattag tttcactcaa actataatcc aactcatcaa taagttttga
     2701 atatatttct tctaattttt ctacttcttc ttcaaatttc tctacattta aattttcttt
     2761 tctaagaatt ttatcttcaa taagcaacat tgaaagttct tctatctttt taaattcatc
     2821 tataacacta tttagataat ttggtctagc aacataataa ttaacaagta ctccaaacaa
     2881 aacacccaca gatgtatcta acaccctatg taaagaataa tacacagggt cactatttcc
     2941 tacacctaga tgtatagcca gaaaagttac acaggcaact actactgact ttattttaaa
     3001 cagattgcat aaatatattg agcacattac tcctagacca cataaaaaag ggtctccagc
     3061 tttaataatg gcaaatatga atcctattat accacctaaa atagtacctt ccactctatt
     3121 gattcctgtc tttaaagaac cttttaccgt atcttgaaca gaaattatac atgctactag
     3181 agaataaaac atattttcta ctaaatagtc accaagaatc atacataaag tgacactaat
     3241 tcctgttttt atagtcctca tacccatctt ttgaagtttc aaagttccac ctcctaaaaa
     3301 gcaccttata taaaatacgc atcctcatat ttaatatgtt cctagtatat cacaattttt
     3361 taaatttacc cattaatcat atttttttat caattctttc ttaatttaga caataaaaaa
     3421 actgctttaa agcagttttt ttatcctatc tcaaccattt taccctcaat tgcaaaaatt
     3481 atcttctcac atacatttgt tatatggtct ccaattcttt caagatatct tcctataagt
     3541 agaagtttta ccccttggtc tatagtattt gagttttcat gcattatttt taaacaatca
     3601 atttgtactt gttcatataa tccatctaca atatcatctt tagttccaac atcataagct
     3661 atacttgcat caaataagat taaagcttct ctagtctgag acatcataga cttacattct
     3721 tttgccatct taggaatatc tatcagctct tttatataag cctctccacc tatcttcaac
     3781 atttctctag ctatatttgc tgcataatct cctattcttt ccaactctat agatatatta
     3841 cctaatgaat aaataaatct taagtctctt gccattggtt gctttaatac tattagttct
     3901 atactcttat ctcttatttc ttctcttaat ttatttattt cctcatcatc ttctactact
     3961 tttcttgcac cctctaaatc cttctctatc atacaagtca cagacttttc tattgcttct
     4021 tcacattttt ccatcataag tactgtttgt tcttttaaag tatcaatact tatatctaaa
     4081 ctattattga ccattgtcta actcctttat agttatatat tttaaccaaa tctacctgtt
     4141 atatagtctt ctgttctttt atctacagga gtggtaaaca tagtttttgt atcgctaaat
     4201 tctataacct ctccatttaa gaaaaaagct gtttcatctg atatacgagc agcttgttgc
     4261 atattatgtg taactatgac tattgtgtag tcttttttta aatcctctat aagttcctct
     4321 acttttaatg ttgaaattgg gtctaaagca gatgtaggtt catccataag aataacttct
     4381 ggtctcatag caattgctct tgctatacat atacgctgtt gttgcccacc agaaagtcct
     4441 aatgcagatg atttaagcct gtctttaacc tcatcccaaa tagctgcacc ttttaagctt
     4501 tcttctacaa ttttatctaa ttgcttctta tctcttagtc catgtgtacg aggaccatat
     4561 gccacattat catatatact cataggaaat ggatttggct tttgaaatac cattcctact
     4621 ttagtgcgaa gatttataac atcatctgag gtatatatgt cctctccatc aacagaaatg
     4681 tttcctttaa ttgttacatc ttcgattagg tcattcattc tatttaacgt tcttataaaa
     4741 gttgattttc cgcatccaga tggacctatt aaagcagtaa ccttattctc ttttatatcc
     4801 atatttattt tctttaatgc ttgcttatca ccataaaata aatccaaatc ttttacactc
     4861 atttttatct tatcgattaa ttccattatt cacacctctt aaatttaata attagcttta
     4921 tttaatttct tagcaacaaa tcttgctaac atgtttagtg ctaatacaat tactattaaa
     4981 acaattccta tactggctgc tgttgctata tctccactct ctttagtcag taagtatgag
     5041 tgtacagtta atgttcttgc actatcaaat attccacctg gcatcttggc tacagtacct
     5101 gctgtaagta atattgcagc tgattctcca attatacgac caactgataa gattactcca
     5161 gataaaattc ctggtattgc acttggaagt actaccttat ataaagtttg aaattttgta
     5221 gctcctaatg ccaatgatga ttctctataa gcttgaggaa ctgtttttaa agcttcttct
     5281 gttgtacgaa ttattactgg taatattatt atcgcaactg taagtgaacc tgcaactatg
     5341 gaatatccca tttttaatgt aactacaaag aaaattcctc caaataatcc atatactata
     5401 gatggtatcc ctgataaact ttcagttgca aatcttatag ctgtaactgc ttttcctttt
     5461 ttagcatatt cttgtaaata tatagcagcc aatattccta taggagttgc tacaactata
     5521 gaaactacta ctgtatataa tgttgtaatt atcattggaa gtataccacc atcacctgat
     5581 gctgaataat tgctaaataa gaaattaaaa ttcatatttc caattccctt tataaatata
     5641 aatcccacta taactactaa tgatactatt gtaaatagtg ctgatagata tactaatgac
     5701 ttcagtagat tctctttgaa ctttctcatc ttaatttacc gccttatttg aaagtttatt
     5761 taaaactaag tttaaaatta atataaatgt aaacaatact actccagttg caaatagcat
     5821 ttcttgatgt gtaccaaacg cgtatcccat ttcaagtgct atattagttg taagtggtct
     5881 tacactatct gttattacag ttggcataac tggtgagttg cctgcaacaa gtattactgc
     5941 cattgtttca cctaaagctc ttcctactcc taacactact gcggctaata ctccagattt
     6001 cgcagcaggt aatacaactt tgaatattgt ttctattttt gatgcaccaa gtgctaatga
     6061 accttctttg tatgcttttg gtactgctct tattgctgtt tctgatacag atattattgt
     6121 cggtaacatc attactgcta gtactattac tattgctaac aagctttgac cttttggaag
     6181 attaaatata ctttgtatag ttggaactat cactgcaaga ccaaatacac catataacac
     6241 tgaaggtata cctgctaaaa gttcaactgc tggtgacatt atttttgcta atcttttagg
     6301 tgctacttct gctataaata cagaggttaa tataccaaca ggtactccta tcacaagtgc
     6361 tccaagtgtt gcaaatattg atgctactat catagatgat attccaaatt tattagcaga
     6421 aggaatccaa tctgcaccag ttaaaaagtc tataaatgaa tatccttcaa atataaatgg
     6481 tcttaatcct ttataaaata cgaatcctat tatcaaaagt aaacttgcaa ctgctactaa
     6541 agcactaagt agaaaaatat tttttgctac tttttcaatt acgtattttg tcttattgcc
     6601 attattgtca tttttttcta aacctactaa tctagatttt actatcataa taccctccca
     6661 ttaaaatata cttatcgatt ttcgttctgt attcaaaatt taatttttta taattcgtct
     6721 tcatcaaatt tcctgtactt ttattctact ttttttaagt taaatctata ttaggattaa
     6781 gttaagcaag tgtaaaatta tatttacttt tttaacatta attttacttt taaaactata
     6841 tttgacattt actttacaat atactgttgt ttctctatat taaaatagca gatatagata
     6901 ataatacagt ttatgtataa tttattacct atatctgcta ttaagatttg ttaactaaat
     6961 ttaactttta tactacatat ataattattg tatcctctag tttatatcac tattatttcc
     7021 atcaaattgt tacgatactt gatgtttgca ataggtacga atagccaaaa atataaaaat
     7081 aggtatttct atcaccgaga aaaacagtat aatgtgtggt gtccaaaaac tcatacctcc
     7141 taagtgtaag aaattaaatg atgtcatttg atataataaa ctaatcttaa aaccaattcc
     7201 ccctggcggt aataggtatc caatccaact aacacctatt cccataggaa ttaagcaaat
     7261 agaaaaagca atcagcatag aagtaagaga atccttacat tttgcagata aaaataatgt
     7321 taaactaacc attgcaagta ttgaaattag accaccaata gctataagaa tctgtgcttg
     7381 tagtaaattt atatttacta gactaatgac agaaaacaac atttgaaatg aggttttcaa
     7441 acattcagta ccaaaagcta aatcagaaat tgccaaatgg atagaaatac atataacaaa
     7501 cattattata aatatagaaa atagagctaa tattttagta aacgctagac gaatatgtcc
     7561 atgtttcgta caacgaagga tgctatctga ttgtgtctga tactcaccag aaaaagtagg
     7621 tgcagcaatt gcaacacata taattaataa tataaaaata taaaatatta tataatcgaa
     7681 agcatcttta gaataacctg ggtatatctc aaatggagta tctacttttc tataaagttc
     7741 ttgagctttc atttgtgcac tggaagtttg tcgttcaatc ttcataatat catttaaatg
     7801 agttttgcat tgttcataaa aagaatctaa atcatttgta tttatttctg ataattctgt
     7861 tgcaaatcct gtttctgaat tagcaaatgc ttcaggaagt ctattcaaca atgggcgaat
     7921 tggtaaaatg cttttagaat aaacatctag tggaaaatct tcactaccta ctttaccata
     7981 cttttcttct gtttttttcc atatatttaa agcatgcttt aatttttttg gtgttacaac
     8041 tccactacta atttcactat tattagcata aattgttttt ttatatttaa tagcatctaa
     8101 accactcaat tcaactacct tgccattatc atctaaataa ttgataccct cataagtaat
     8161 tggaagaaaa gccatcaatg cagataaaac caacgccaac aataataaaa ttaatgtacg
     8221 acgtgagtgc aacactcgtt taatttccaa ataaaaaata cgcatttatt tcccctcctt
     8281 actataaact gcttctggaa ataaccataa atataaatct tctaatcgtg gttcaacagc
     8341 aatagagtta tcaatttgtg acttttctgc caagtaacga atagatactt gattgttttt
     8401 ttcactacgc tgattgatta tacgtaaatt catttcatat tttgaaatat ctctatttga
     8461 tataagacat tcccatactt ttccctctac tttcttaacc agtttatcgg ttgttcccac
     8521 atcaagaatt tttccacttt tcataatagc attttgagtt gatatgtact caatatcaga
     8581 tacaatgtga gtagatatca ggacaacacg attatgagaa aattctgaaa taaaattacg
     8641 aaatctcact cgctcaccag ggtcaagccc tgctgttggt tcatccatta taaggatttt
     8701 agggtcattt aacatggctt gtgcaattcc cactcgtcgt ttcattccac cagataattt
     8761 aataatcttt ttatttttta catcagataa tgtaaggatt tccaatagat tattaatttt
     8821 tccttttact tctcttggtg gtacatcttt caatgctgct atatattcta aataatcttt
     8881 tacagaaaaa tcctgtggga aaccaaattc ttgtggtaaa aatcccagta aatttctgta
     8941 ctcagcaccc attgtataaa tattttttcc attatacaga attttgccat tggtaggttt
     9001 cataatgtct gtaataattc tcataagggt agttttacct gctccgttcg ctcctagaaa
     9061 tccccacaca cctggtgtta aagtaaggca aacatcttct acagcaattt tatccttaaa
     9121 ttttttcgtc acatgttcaa tttttaattc catatttgaa actcctctct ttatatactt
     9181 acctgcaaaa acaaaagtgc cttgctcagt atttatttta ctaaacaaag acactcatag
     9241 ttttaatatt aaatagataa tttcttaaga aaatcctaat atttgattta tattttacta
     9301 aatagatgta ggaagcgata tagtaaatgt tgtgacttca tctttacttt gaacagtaat
     9361 atcaccacca tgcaaattta taatttcctt agctatagaa agccctaaac ctgcaccacc
     9421 catatcagat tttctagctt catctaaacg attgaacttt tcaaatatag cagaaagttg
     9481 ttcttccgga attgtctgtc cataatttct aaaagcaata tctaccttgc tgtctttatg
     9541 aaccgctgag ataataattt ctgtattagg atagctgtaa gcaatagcat ttttcaagat
     9601 attattaaat actcttgcaa gtttttcaga atctgctttt attgttaaac tttcatttgc
     9661 ttttagaatg gctctatttc ctttttcaga tagcaatggg taaaattcat cctttaactg
     9721 aagtaacata taatataggt ctactttttc gaaattaatt ttgatttgat tagaattata
     9781 tcgtgttatt tcaaaaagtt cattgataag tttttctaaa tattgtgctt tgtcaagtgc
     9841 aacttcagta tacttagatt tttgagaatt atccatatca ggtatttctt ttaataagct
     9901 caaataccca ataactgaag taagtggtgt ccttatatca tgtgctagat acatcacaag
     9961 gttattcttt ttattttcag cctctttcac tgcttgttga cttaacaaaa cagacatctt
    10021 aatttggttc atttgttgct ctatttcctt taatggctta gatagttcaa ccaattcatc
    10081 attttgttga taaatagtat gtgttgcatt gatgatttct cttaaataat tccatggttt
    10141 tttccaataa aaattgaaaa tagtgatata tccaattata aaaaccaaaa aaaataatac
    10201 atctcttctc aacttaagcc atttaacaag aggatttgaa aaatttgaaa acgtataatc
    10261 agccataaac attaaaacca atactatacc tgtatagata gctaacatta tatataattt
    10321 atactttaaa tacgcctctg tatttttttc tctttttata aattcatatt tatctcttag
    10381 tttcctataa gtaaaattat taatcttttt cataatacta catcctttct tgacaagaaa
    10441 aataggtgtc aaatcttgta accaacaccc caaattgttt ttatatattt tgcttcatct
    10501 acagtatcac ctaacttttc acgaagatgg cgaatatgaa ctgttatagt attgttactt
    10561 ttattgtaat attcatcttt ccagatagca tgaaaaagat tttctgaatt taccacagag
    10621 cctttatttt ttaataatac atgtagaata gaaaactctg ttggagtaag tgaaagagct
    10681 ttaccattta aatgacattc atatgtttgg gtattcaatt ccagacctaa aaaagaaaaa
    10741 atatgtgaaa gttcttcacc agattgtaaa cttccactgt acttcttata tcttctaagc
    10801 tgtgccttaa ctctagcaac aagttccaaa ggtcggaaag gtttagtaac ataatcatct
    10861 gcacctaaag ttaagcctgt aatcttgtcg gtttcttcat ccttagctgt taacataatg
    10921 attggataag tatatttttc tcggattttt tggcataaag aaaatccatt tactcctggc
    10981 aacataacat ctaaaattgc aaggtcaaat tcatttaaat taatgcagtt tagtgcatca
    11041 agaccagaat aaaatttata aacaatatag ttttcatttg taaggtagac ttctattaaa
    11101 tcagctatat cacgttcgtc atctacaact aaaatttttc cattcataga ctttaaacat
    11161 cctttctat
//
LOCUS       DAEPXF010000125        11140 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain HB27
            SAMN13376389-rid12479423.denovo.132, whole genome shotgun sequence.
ACCESSION   DAEPXF010000125 DAEPXF010000000
VERSION     DAEPXF010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13376389
            Sequence Read Archive: SRR10518432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 11140)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11140)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001069624.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001069624.1
            Genome Coverage       :: 315x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/15/2021 01:54:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,165
            CDSs (total)                      :: 4,076
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 89
            rRNAs                             :: 10, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 10 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 19 of 69
            Pseudo Genes (incomplete)         :: 44 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 10 of 69
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11140
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13376389-rid12479423.denovo.132"
                     /strain="HB27"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="China: Hebei"
                     /collection_date="2017"
     gene            419..1741
                     /gene="dnaA"
                     /locus_tag="KRL67_002759"
     CDS             419..1741
                     /gene="dnaA"
                     /locus_tag="KRL67_002759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000428021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HBH1523625.1"
                     /translation="MMDIVSLWDKTLQLIKGDLTSVSFNTFFKNIVPLKIHLNDLILL
                     APSDFNKDILENRYLHLIEDAISQLSLKKYNIKFVLSEKEVADLNSDSTDLNYRVLYP
                     NLNPKYTFDTFVIGNSNRFAHAACVAVAESPAKAYNPLFLYGGVGLGKTHLMHAIGHH
                     IVSQKKDSKVVYVSSEKFTNELINSIKDDKNEEFRNKYRNVDVLLIDDIQFIAGKERT
                     QEEFFHTFNTLHEANKQIIISSDRPPKDIPTLEDRLRSRFEMGLITDIQAPDFETRIA
                     ILRKKAQLERIDVPNEVMSYIAKNIKSNIRELEGALTRVVAYSSLSNRVISFDLATEA
                     LKDIITTSKNEEINVLRIKEKVSSVFNLKMEDFNSKKRTRSIAYPRQIAMYLTRELTD
                     LSLPKIGEEFGGRDHTTVIHAHDKVSKDIEESEEIKTKIDKIISDLKG"
     gene            1983..3089
                     /locus_tag="KRL67_002760"
     CDS             1983..3089
                     /locus_tag="KRL67_002760"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HBH1523626.1"
                     /translation="MKIICNQKILANRIGIAQKAINGKTTIELLKGILISTEEGQLKL
                     TGYDAEIGIETYVQAEIIEKGDVVVDARLFGDIIRKLPDSFVEIETDSENNIYINCVN
                     SRFKIKGYAAKEFPKLPELNEEDLYSIPQEILKNMIKQTVFAISQDQTKPVLMGELLE
                     IVDRNLNLVAIDGYRLAVKSCSVDSLTENIKVIIPGKTLIDVNSLLSGEDNVKVGFNE
                     KNAIFIINDTKIITRLLEGDFIDYKKLLPREHNSRVKLNTKELLNSIERASLLSQSEK
                     NNLIKLSIRDKVMAITSNTEKGNVYEEVEIDLDGDYLDIAFNSRYFIEGLKNIDNEEI
                     FIEFTTNVNPCIIKPTDDVNYIYLLLPVRISSNI"
     gene            3226..3432
                     /gene="yaaA"
                     /locus_tag="KRL67_002761"
     CDS             3226..3432
                     /gene="yaaA"
                     /locus_tag="KRL67_002761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein YaaA"
                     /protein_id="HBH1523627.1"
                     /translation="MTEITIESEYIKLDQFLKLAEIASTGGHAKFLIQEGLVTVNDEI
                     ELRRGKKIKSGDIVEIEGTKIKVL"
     gene            3450..4565
                     /gene="recF"
                     /locus_tag="KRL67_002762"
     CDS             3450..4565
                     /gene="recF"
                     /locus_tag="KRL67_002762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HBH1523628.1"
                     /translation="MKLKSLQLVNFRNYKKLHLEFNGKVNLLVGKNGQGKTNIVESIY
                     MLSFGKSFRTNKDKEMVRFNSENLYIGGSFSKYNKYSLIELIIGKDKKGIRINKVPLQ
                     KIQELLGNLNVVIFSPEDLRLVKEGPKERRAFIDKEISQIIPKYYKYLTNYNKTLSQR
                     SRVLKNIHVDEALLDVYDDTLAKYGSYIYILRRDFIKKIANISENMHMNLTNGVERLS
                     IRYKNQINITDEDTIDTVYNKFLAKLSSNRPNDIESKTTRYGIHKDDLNIFINDLDAR
                     LFGSQGQQRTASISLKLSEIELIKNEVEEYPVLILDDVFSELDEARQKLLVNNLSNVQ
                     MFITSAEVSHKKIFDEKNVTIFNIENGDVISIENGGN"
     gene            4566..6467
                     /gene="gyrB"
                     /locus_tag="KRL67_002763"
     CDS             4566..6467
                     /gene="gyrB"
                     /locus_tag="KRL67_002763"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003226808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HBH1523629.1"
                     /translation="MKQEYGASQIQVLEGLEAVRKRPGMYIGSTSPRGLHHLVYEVVD
                     NSIDEALQGYCSDIYVSINEDGSVLVKDNGRGIPVEIHPKTGKSTLETVLTNLHAGGK
                     FGGGGYKVSGGLHGVGVSVVNALSKWMVAEVYLNGKIYKQTYEKGLPTSKLEVVGESQ
                     DKGTMIQFMPDETIFDEIEFKYETLEYRLRELSFLNKGIKIVFEDKREGQEKRKEFHY
                     TGGLVEYIKYLNKSRTGIHDDIVYIDKKVDDCFVELAMQYTDGYTENIYSFANNINTH
                     EGGSHLSGFKAALTKTVNDYAKRNKFLKENDVNLLGEDIREGLTAVVSVKLPEPQFEG
                     QTKTKLGNSFMRGIVDSVTVDELGSFLEENPATARIIVDKALRAQRAREAAKKARELT
                     RRKSVLESTSLPGKLADCAEKDPSKSEIFLVEGDSAGGSAKQGRDRNSQAILPLRGKI
                     LNVEKSRLDRILSSDEIKNMITAYGCGIGEDFDIDKARYHKIIIMTDADVDGAHIRTL
                     LLTFFFRYMRPLIDEGYVYAAQPPLYKVTKQKKEHYVYSDKELNILLDEIGRNGVELQ
                     RYKGLGEMNAEQLWETTMNPETRTLLQVTVEDAAIADEVFSMLMGDKVAPRKEFIEEN
                     ARFVRNLDI"
     gene            6487..8913
                     /gene="gyrA"
                     /locus_tag="KRL67_002764"
     CDS             6487..8913
                     /gene="gyrA"
                     /locus_tag="KRL67_002764"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HBH1523630.1"
                     /translation="MEENNKILPIEIAEEMKKSYIDYSMSVIAGRALPDVRDGLKPVH
                     RRILYSMSELNLTPDKPYRKSARIVGDVLGKYHPHGDIAVYYAMVRMAQDFSTRALLV
                     DGHGNFGSVDGDSPAAMRYTEAKMSKLSLELLRDIEKETVDFKPNFDESLKEPSVLPA
                     RYPNLLVNGSNGIAVGMATSIPPHNLAEVIDATVYLIDNPECSVDDLIKFVQGPDFPT
                     AAIIMGKESIAEAYRTGRGKVKVRSRAFIEELPKGKQQIIVTEIPYQVNKAKLVERIA
                     ELVKEKRIEGISDLRDESNRNGMRIVIELKRDANANIVLNNLYKHSQMEDTFSIIMLA
                     LVDGQPRVLNLKQILYHYIKHQEDVVTRRTKFELNKAEARAHILEGLKIALDNIDAVI
                     SLIRASKTGQEAKLGLIEKFKLTEIQAQAILDMRLQRLTGLERDKIEAEYEDLIKKIN
                     RLKEILADERLLLNVIKDEITIIKENYSDERRTEIRHAEGEIDMRDLISDEEIAITLT
                     HFGYIKRLPSDTYKSQKRGGRGISALTTREEDFVRHLVTTTTHSRLLFFTNKGRVFKL
                     NAYEIPEGKRQAKGTAIVNLLQLSADEKIATLIPIDGNDENEYLLLATKKGIVKKTKR
                     EEFKNINKSGLIAIGLRDDDELIGVELTDGKQEVLLVTKEGMSIRFDENDIRYMGRTA
                     MGVKGITLSKEDFVVSMNLCSKGTDVLVVSKNGFGKRTNIEEYRSQIRAGKGIKTYNI
                     SEKTGTIVGADMVNEDDEIMIINSDGVLIRIRVNEISLFGRVTSGVKLMKTNDEVNVV
                     SIAKINIEEE"
     gene            8982..9278
                     /locus_tag="KRL67_002765"
     CDS             8982..9278
                     /locus_tag="KRL67_002765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HBH1523631.1"
                     /translation="MCNKRSCKKGGFLLLGAILGFIFGMFFAPKKGSELRKETKEKFN
                     DVKENPKEVLHETFNDVKERIINLVDDDNNEEDIKISEEDIVISKSFDDEGDVN"
     gene            9278..9538
                     /locus_tag="KRL67_002766"
     CDS             9278..9538
                     /locus_tag="KRL67_002766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF948 domain-containing protein"
                     /protein_id="HBH1523632.1"
                     /translation="MNAWGWQIGAVLVGSSALIVAIYLAKTLNSINKVVEKAYKIVDY
                     NERHIQDIVENASSISKGIDDIVYVINKILSIGNIFKIIKRK"
     gene            9550..9867
                     /locus_tag="KRL67_002767"
     CDS             9550..9867
                     /locus_tag="KRL67_002767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HBH1523633.1"
                     /translation="MSMNIDSNLDSQNKFWNVCLDGELDVSTADKLKEHLHALIEKNM
                     LDVKINLKDLDYIDSTGLGAMIGVLKKLKINEKEIYIVNPKSNVRKIFTITGLDKIFK
                     VEG"
     gene            9892..10278
                     /locus_tag="KRL67_002768"
     CDS             9892..10278
                     /locus_tag="KRL67_002768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBH1523634.1"
                     /translation="MEITTNPDYVSIIRLTASGIANKMGFPIDDIEDIKVAVSEACTN
                     AIKHSKDDVFNIVFNILDNAINIEIQDNGKGYDVSSISTPDLENPKESGLGLFIIKTL
                     MDDVDIESEHNQGTKIKMTKYLGVDI"
     gene            10278..11051
                     /locus_tag="KRL67_002769"
     CDS             10278..11051
                     /locus_tag="KRL67_002769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SigB/SigF/SigG family RNA polymerase sigma
                     factor"
                     /protein_id="HBH1523635.1"
                     /translation="MKNVANATHYLNMDTKELFNLYNKNKNVDIRNILIERHLYLARL
                     LAKKYINKGVDFEDIYQVASLALIYAIDRYDVEKGFEFSSFATPTIVGEIKKYFRDKV
                     WTLRVPRRIQELSKKISDAKIKLEQENKKHPKVKDIADYIGVSEEDVLEAMEASYGYQ
                     PMSLDSSSNDDSEDKDITLIDKIGKEEGNFGSIEYEDFINKFIETLNELEVKIFKDRF
                     FFDKTQSSIAKELGISQMTVSRLEKKVVEKLKKEYEKNL"
BASE COUNT         4536 a         1153 c         2020 g         3431 t
ORIGIN      
        1 aagtaatttt aacatgttta tatttttatc aacaattagt taatattgtg gataaattag
       61 ttacaaatca ttgaagtttg acatatattt tgatattata aatatacttg ttgataatgt
      121 gcataaatgt tatcaactta ttagaaatta ttcacagctt gtggatactt ttgtgtataa
      181 cttttaagat tactgacaat actctagtta taatcaccat ttattgtatg tttatgttat
      241 ttataacttt atctacatat tgtttatatc ttttcatatt tttttaataa tttatactat
      301 tattaattta tcaacaccat tatccataaa tttagttatt ctgtaaaatt tatatatatt
      361 tttgcttttc tgttaataac tttgtggaaa atattaatta ttctgttcgg aggtttatat
      421 tatggatata gtttctttat gggacaaaac cctacaatta ataaaaggtg acttaacttc
      481 agtaagtttt aatacctttt ttaaaaacat cgtaccatta aaaatacatc ttaatgattt
      541 aatattactg gctcctagtg attttaataa agatatctta gagaatagat atttacattt
      601 aattgaagat gctattagtc agctgtcatt aaaaaaatat aatattaaat ttgtattgag
      661 cgaaaaagaa gttgctgatt taaattctga tagcacagat ttaaattata gagttcttta
      721 ccccaatcta aatccaaaat acacatttga tacattcgtt attggtaata gcaatagatt
      781 tgcacatgct gcatgcgttg cagttgcaga atctccagct aaagcttaca acccactttt
      841 tttgtatgga ggagttggat taggtaagac tcacttaatg catgctatag gtcatcatat
      901 tgttagtcaa aaaaaagact caaaagtagt ttacgtttct tcagaaaaat ttactaatga
      961 acttataaac tctataaaag acgataaaaa cgaagaattt aggaataaat atagaaacgt
     1021 agatgtatta ttaatcgacg atattcaatt tatagcagga aaagaaagaa ctcaagaaga
     1081 gtttttccat acattcaata ctcttcatga agcaaacaaa caaataatta tatctagtga
     1141 tagaccgcca aaagacatac ctacactaga agataggcta agatcgagat ttgaaatggg
     1201 acttataact gatattcaag cacctgactt tgagacaaga attgctatac ttagaaaaaa
     1261 ggctcaacta gaacgtatag atgtgccaaa tgaggttatg tcatatatag caaaaaatat
     1321 taagtcaaac ataagggaac tagaaggtgc attaactaga gtggttgcct attcttctct
     1381 tagcaatagg gtaatatctt ttgatcttgc tacagaagct ttaaaagata ttataacaac
     1441 ttctaaaaat gaagaaataa atgttctcag aataaaagaa aaagtatctt ctgtattcaa
     1501 tttaaagatg gaagatttta attctaaaaa aagaacacgt tcaatagctt atccaagaca
     1561 gatagctatg tatttaacgc gcgaacttac tgatttatcc cttcctaaaa taggtgaaga
     1621 gtttgggggt agagaccata caacagtaat tcatgctcat gacaaagtat ctaaggatat
     1681 agaagaaagt gaagaaatta agactaaaat agacaaaatt atatcagatt tgaagggata
     1741 atttatacac aattgtatgt ttatatctct tgaataacct gttaatactt tttttgaaca
     1801 aaaattatta acaaactatc aagtctaaaa taacaaagat attaacatct tatacacatg
     1861 ttaatatctt tgtatttata atgttataca tacttattaa cattatcaac aacacctact
     1921 actattacta ctatgatttt aacttatttt tatatataaa aagccagagg aggttatcca
     1981 cattgaaaat aatttgtaat caaaaaatcc tagcaaatag aattggaatt gctcaaaaag
     2041 ctattaatgg aaaaacaaca atagaattat taaaaggtat acttatatct acagaagaag
     2101 gtcaattaaa acttactgga tatgatgctg aaataggaat agaaacttat gtacaagcag
     2161 aaatcataga aaaaggtgat gttgttgtag atgcaaggct atttggagat ataattagaa
     2221 aattaccaga ttccttcgtt gagatagaaa ctgattcaga aaataatatt tacataaatt
     2281 gtgtaaattc aagatttaaa attaaaggtt atgcagcaaa agaatttcct aaattaccag
     2341 aattaaatga agaagattta tatagcatac ctcaagaaat cctaaaaaat atgattaaac
     2401 aaactgtatt tgctatctca caagaccaaa caaaacctgt tttaatggga gaacttttag
     2461 aaattgtaga tagaaactta aatttagtag caattgatgg atatagatta gctgtaaaaa
     2521 gttgttcagt tgatagttta actgaaaata taaaagtcat aattcctgga aagacactta
     2581 tagatgtaaa tagtttgctt tctggagagg ataatgttaa agttggattt aacgaaaaaa
     2641 atgctatttt tataattaat gatacgaaaa ttattacaag attgcttgaa ggagatttta
     2701 ttgattataa aaaattattg ccaagagaac ataacagcag agttaaatta aacactaagg
     2761 aacttttaaa tagtatagag agagcgtctt tattgtctca atcagaaaaa aataatctta
     2821 taaagttatc tataagagat aaagtaatgg caattacttc taatacagaa aaaggaaatg
     2881 tgtatgaaga agttgaaata gatttagatg gagattatct agatatagct ttcaattcta
     2941 gatattttat tgaaggtttg aaaaatatag ataatgagga aatatttata gaatttacta
     3001 caaatgtaaa tccttgtata attaaaccaa cagatgatgt taattatatt tacttactgc
     3061 ttccagtaag aatatcatca aatatatagc agacatgaag gagttgtctt ttgtagaaaa
     3121 tttttctaat cttagacaac tccttttata agcatattta tggtataata tacttttagt
     3181 gtatcaatag gaatttgttt aaaatagtga caaggaggaa ctattatgac tgaaataact
     3241 atagaatcag aatatataaa gttggatcaa tttcttaaat tagcagagat tgcatcaaca
     3301 ggaggtcatg caaaattttt aattcaagaa ggtttagtga cagtaaatga tgaaatagaa
     3361 ttaagaagag gaaaaaagat aaaatcagga gatatagttg aaatagaagg aactaaaata
     3421 aaagtattgt aaaaaaagga gaactttttg tgaaacttaa aagtttgcaa cttgttaatt
     3481 ttagaaatta taaaaagttg catctggaat ttaatgggaa agtaaatcta cttgttggta
     3541 aaaatggtca aggaaagaca aatatagtag aatctattta tatgctttct tttggaaaat
     3601 catttaggac taacaaagat aaagaaatgg ttagatttaa tagtgaaaac ttatatattg
     3661 gaggaagttt ttcaaaatat aataaatata gtctaataga gctaattatt ggaaaagata
     3721 aaaaggggat aagaataaat aaagttcctt tacaaaagat acaagaacta cttggcaact
     3781 taaatgttgt tatattttct cctgaggatt taagattggt taaagagggt cctaaagaaa
     3841 gaagggcatt tatagacaaa gagattagtc agattatacc taaatattat aaatatctta
     3901 ccaattataa taaaacactt tctcaaagga gtagggtgct taaaaatata catgttgatg
     3961 aagctttatt agatgtatat gatgatacgt tagcaaagta tggaagttat atttatattt
     4021 tgcgtagaga ctttataaaa aagatagcta atatatctga aaatatgcat atgaatttga
     4081 ctaatggagt tgaaagatta tcaattcgat ataaaaatca aataaatata actgatgaag
     4141 atactataga tacagtatat aataaatttt tagccaagtt gtcatcgaat agacctaatg
     4201 atatagaatc taagactacc agatatggta tacacaaaga tgacttaaat atattcataa
     4261 atgatttaga tgcaagactt tttggttcac aaggtcaaca aagaactgct tcaatatcat
     4321 taaagttatc agagatagaa ttaataaaaa atgaagttga agaatatcca gtattaattt
     4381 tagatgatgt ttttagtgag ttagatgaag caagacaaaa acttttagta aataatttaa
     4441 gcaatgttca aatgtttata acaagtgctg aggtttcaca taaaaaaata tttgatgaaa
     4501 aaaatgttac aatatttaat attgaaaatg gcgatgttat tagcatagag aatggaggaa
     4561 attaaatgaa acaagaatac ggtgcaagtc agatacaagt attagaggga ttagaagccg
     4621 ttagaaaaag accaggtatg tatattggta gtacaagccc tagaggttta catcacttag
     4681 tttatgaagt tgtagataat agtatagatg aggctttaca aggatattgt tcagacatat
     4741 atgtatctat taatgaagat ggcagtgttt tagtaaaaga taacggaaga ggtatacctg
     4801 ttgaaataca tcctaaaact ggtaaatcaa ctttagaaac agtattaact aatcttcatg
     4861 caggaggaaa gtttggaggc ggaggatata aagtatctgg aggtcttcat ggagttggtg
     4921 tttctgtagt taatgcattg tcaaaatgga tggtagcaga ggtttactta aacggaaaga
     4981 tatataagca aacatatgag aaaggattac caacgtctaa gcttgaagta gttggagaat
     5041 ctcaagataa aggaacaatg attcaattta tgccagatga gacaatattt gatgaaatag
     5101 agtttaaata tgaaacattg gagtataggc ttagagaact ttctttcttg aataaaggaa
     5161 taaaaatagt atttgaagat aaaagagaag gacaagaaaa aagaaaagaa tttcattata
     5221 caggtggttt ggtagaatat attaagtatt taaataaatc tagaactggt atacatgatg
     5281 acatagttta tatagataaa aaggttgatg attgttttgt tgaactagct atgcaatata
     5341 cagatggtta tacagagaac atatattctt ttgcaaacaa tataaatacg cacgaaggag
     5401 gttctcattt aagtggattt aaagcagctc ttactaaaac tgtaaatgat tatgctaaga
     5461 gaaataaatt tttaaaggaa aatgatgtta acctacttgg tgaagacata agagaaggtc
     5521 ttacagcagt agtgtcagtt aaattaccag aacctcaatt tgaaggtcaa actaaaacaa
     5581 agctaggtaa tagctttatg agaggtatag ttgatagtgt aacagttgat gaactggggt
     5641 ctttcctaga agaaaaccca gcaacagcaa gaataatagt tgataaagct ttaagagcac
     5701 aaagagctag agaagctgca aaaaaagcaa gagaattaac aagaagaaaa agtgtattgg
     5761 aaagtacatc tttacctgga aaacttgcag attgtgcaga aaaagatcca tctaaaagtg
     5821 aaatattctt agtcgaaggg gattcagcgg gaggttcagc taaacaaggt agagatagaa
     5881 atagtcaagc tatacttcca ttaagaggta aaatacttaa tgttgagaaa tcaagactag
     5941 ataggatatt atcttcagat gaaataaaaa atatgataac agcttatggt tgtggtattg
     6001 gagaagattt tgatatagat aaggctaggt atcataaaat tataattatg accgatgccg
     6061 atgtagatgg agcacatata agaactttat tattaacatt cttctttaga tatatgagac
     6121 ctcttataga tgaaggatat gtttatgcag cacagccacc tttatataaa gtgacaaagc
     6181 aaaagaagga acattatgtt tactcagata aagagttaaa tatcttatta gatgaaattg
     6241 gaagaaatgg tgttgaactt caaagataca aaggtcttgg agagatgaat gcagagcaat
     6301 tatgggaaac aactatgaat cctgagacaa gaactttatt acaagttact gtggaggatg
     6361 ctgcaattgc agatgaagta ttttcaatgc ttatgggtga taaggttgct ccaagaaaag
     6421 aatttataga agaaaatgca agatttgtta gaaacttaga tatatagaga gaggggatta
     6481 gtatacatgg aagaaaataa caaaatactc cctattgaaa tagcggaaga aatgaaaaaa
     6541 tcgtatattg attattcgat gagtgttata gctggacgtg ctcttcctga tgttagagat
     6601 ggtttaaagc cagttcatag aagaatatta tattcaatga gtgagttaaa tttaactcca
     6661 gataaaccat acaggaagtc agctcgtatt gttggggacg ttttaggtaa gtaccatcct
     6721 catggagata ttgctgttta ttatgctatg gtaagaatgg cacaagattt ttcaaccaga
     6781 gcacttttag tagatggtca tggtaacttt ggttctgttg atggggattc accagctgct
     6841 atgcgttata cagaagctaa aatgagtaaa ttatcattag aactactgag agatattgaa
     6901 aaggaaactg tagactttaa accaaacttt gatgagtcat taaaagagcc ttcagtattg
     6961 ccagctagat atcctaattt attagtaaat ggctcaaatg gtatagctgt tggtatggca
     7021 acttcaatac ctccacataa tttagcagaa gtaattgatg caactgtata tttgatagat
     7081 aatccagagt gtagtgtaga tgatttaata aaatttgttc aaggaccaga tttccctaca
     7141 gctgcaatta taatgggaaa agaaagtata gcagaagcat acagaactgg aagaggaaaa
     7201 gttaaagtta ggtctagagc ttttatagaa gaactaccaa aaggaaaaca gcaaataata
     7261 gttacagaaa taccttatca agtaaataag gctaaactgg ttgagagaat agcagagtta
     7321 gttaaagaaa agagaataga aggtatatca gacctaagag acgaaagtaa tagaaatggt
     7381 atgagaattg ttatagaatt aaagagggat gctaatgcta atatagtatt aaataatttg
     7441 tataaacatt ctcaaatgga agatactttt agcataataa tgcttgcact tgtagatggt
     7501 cagccaagag ttttaaatct taaacaaata ttatatcatt atattaaaca tcaagaagat
     7561 gttgttacaa gaagaactaa atttgaacta aataaagctg aagcaagagc acatatttta
     7621 gaaggattaa agattgcttt agataatata gatgctgtta taagcttgat aagagcttca
     7681 aagactgggc aagaagctaa gctaggttta atagaaaaat tcaaattaac tgaaatacaa
     7741 gcacaagcta tattagatat gagacttcaa agacttacag gtttagaaag agataagata
     7801 gaagctgaat atgaagattt aatcaagaag ataaatagat taaaagaaat tttagctgat
     7861 gaaagattac ttttaaatgt aataaaggat gaaattacaa taataaaaga aaattactct
     7921 gatgagagaa gaacagaaat aagacatgct gaaggcgaaa tagatatgag agatcttata
     7981 agtgatgaag aaatagcaat aactcttact cactttggat atataaaaag gcttccatct
     8041 gatacttata agagtcaaaa aagaggtgga agaggtattt cagcacttac aactagagaa
     8101 gaagattttg taagacactt ggtaactaca actactcata gtagactatt attctttaca
     8161 aataaaggta gagtatttaa attaaatgca tatgaaatac cagaaggtaa gagacaggct
     8221 aaagggactg ctatagtgaa tttacttcaa ttgtcagcag atgagaaaat tgctactcta
     8281 atacctattg atggtaatga tgaaaatgaa tatttattac ttgcaacgaa aaagggtatt
     8341 gttaaaaaga ctaagagaga agagttcaaa aatataaata aatctggtct tattgctata
     8401 ggcttaagag atgatgatga gcttattgga gtagaactta cagacggaaa acaagaagta
     8461 cttttagtaa ctaaagaagg tatgtctata agatttgatg aaaatgatat aagatatatg
     8521 ggtagaacag caatgggtgt aaaaggtata actttaagta aagaagattt tgttgtgtct
     8581 atgaatcttt gcagtaaagg tacagatgtg ttagttgtaa gtaagaatgg ttttggaaag
     8641 agaacaaata tagaagagta tagaagtcaa ataagagctg gtaaaggaat taaaacttat
     8701 aatatatctg aaaagactgg tacaattgta ggtgcagata tggtcaacga agatgatgag
     8761 ataatgatta taaattctga cggagttctt attagaataa gagtcaatga aatatcactg
     8821 tttggaagag ttacaagtgg tgttaaatta atgaagacta atgatgaagt caatgtagtt
     8881 tcgattgcca aaataaatat tgaagaagaa taggataaat atcctattct ttttgtataa
     8941 tgtagttaga taaaaattta aaattgaaag gagtaattgt tatgtgcaat aaaagaagtt
     9001 gtaaaaaagg tggattttta ttattaggag ctattttagg gtttatattt ggtatgtttt
     9061 ttgcaccaaa aaaaggttct gaactaagaa aagaaacaaa agagaagttt aatgatgtca
     9121 aagaaaatcc aaaagaagtt ctacatgaaa catttaatga tgttaaagag agaataataa
     9181 atttagtaga cgatgataat aatgaagaag atataaaaat ttcagaagaa gatatagtaa
     9241 taagtaaaag ctttgatgat gagggagatg ttaactaatg aatgcatggg ggtggcaaat
     9301 tggagcagtg ttagtaggaa gctctgctct tatagtagca atttatttag ctaaaactct
     9361 aaacagcata aataaggttg ttgaaaaggc atataagatt gtagattata atgaaagaca
     9421 tatacaagat attgttgaaa atgcatcatc aatatcaaag ggaatagatg atatagttta
     9481 tgtaattaat aaaatattga gtataggaaa tatatttaaa attattaaaa ggaaatagga
     9541 ggtttttata tgtctatgaa tattgattca aatttagatt cacaaaataa attttggaat
     9601 gtttgtcttg atggagaact tgatgtttct acagcagata aactaaagga acatttacat
     9661 gctttaatag aaaaaaatat gttagatgta aagattaatc ttaaagattt agattatata
     9721 gactcaacag gtttaggagc tatgataggt gtattaaaaa aattaaaaat aaatgaaaaa
     9781 gagatataca tagtaaatcc taagagtaat gtaagaaaga tttttactat aacaggtcta
     9841 gataaaatat ttaaagtgga gggataaact ttggcttgtg agactataaa gatggaaatt
     9901 actacaaatc cagattatgt tagtataata agattaacag catctggaat tgccaataag
     9961 atgggttttc caatagatga tatagaagat ataaaagtag cagtatctga agcatgtaca
    10021 aacgcaataa aacacagtaa agatgatgtg tttaatatag tatttaatat tttagataat
    10081 gcaattaata tagaaattca agataatgga aaaggatatg atgttagctc aataagtaca
    10141 ccagatttag agaatccaaa agagagtggt cttggattgt ttataattaa aactttaatg
    10201 gatgatgtgg atattgaatc agaacataat caagggacaa aaataaaaat gactaaatat
    10261 ttaggagttg atatttaatg aaaaatgtag ctaatgctac acattaccta aatatggata
    10321 caaaagagct atttaattta tataataaaa ataaaaatgt agacattaga aatattctta
    10381 ttgaaagaca tctgtattta gcaagattgt tagctaagaa gtatataaac aaaggtgttg
    10441 attttgaaga tatatatcaa gttgcttcat tggcattaat ttatgctatt gatagatatg
    10501 atgttgagaa aggatttgag ttctccagct ttgcgacacc aactatagta ggagaaataa
    10561 aaaagtattt tagagataag gtatggacac ttagagtccc aagaagaata caggaattaa
    10621 gcaaaaagat aagtgatgcg aaaattaaat tagaacaaga aaataagaag catccaaagg
    10681 taaaagatat agctgattat ataggtgtta gtgaagaaga tgttttagag gccatggagg
    10741 cttcttatgg atatcaacca atgtctttgg attcatcaag taatgatgat tcagaggata
    10801 aagatataac tcttattgat aagattggaa aagaagaagg taactttgga agtatagagt
    10861 atgaagattt tattaataag tttattgaaa ctttaaatga attggaagtt aaaattttta
    10921 aagatagatt tttctttgat aaaacacaat ctagtatagc taaagaacta gggatatcac
    10981 agatgactgt ttctagacta gagaaaaagg tagtagagaa gctgaaaaaa gaatatgaaa
    11041 aaaatttata aaaaaatata aaaaaagtat tgacctactg ttttaaagat ggtatagtat
    11101 tacttgtcct tgaaaaaagg aacaagaaat aaacacgaac
//
LOCUS       DAEPXF010000126        11115 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain HB27
            SAMN13376389-rid12479423.denovo.133, whole genome shotgun sequence.
ACCESSION   DAEPXF010000126 DAEPXF010000000
VERSION     DAEPXF010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13376389
            Sequence Read Archive: SRR10518432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 11115)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11115)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001069624.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001069624.1
            Genome Coverage       :: 315x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/15/2021 01:54:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,165
            CDSs (total)                      :: 4,076
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 89
            rRNAs                             :: 10, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 10 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 19 of 69
            Pseudo Genes (incomplete)         :: 44 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 10 of 69
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11115
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13376389-rid12479423.denovo.133"
                     /strain="HB27"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="China: Hebei"
                     /collection_date="2017"
     gene            478..1338
                     /locus_tag="KRL67_002770"
     CDS             478..1338
                     /locus_tag="KRL67_002770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HBH1523636.1"
                     /translation="MKIIQILNNNVALVKRGKNEIIVFVKGIGFKKRVGQVINENEIE
                     RIYILDSYDMLEHFSYLLQNSDSNNIVLINEIIEYGEKALNIKSSDYLSLTLLDHLEF
                     LLKRCEKNQFIKSPLVWNVKRFYPKHFEVGMHALKLIGDSKGINLPDDEAVSIALHFV
                     NMEVNKESHDSTIVELRTLADIVSIIKYHFNVELDETSTNYMRFTTHLQYFIQRIMSD
                     EASIDEDNSIDLYKQVSQLYPKSFGSVQKIRKYIKSQFNVDISISEETYLMLHINRVT
                     QRLEAKSDEI"
     gene            1328..3199
                     /locus_tag="KRL67_002771"
     CDS             1328..3199
                     /locus_tag="KRL67_002771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HBH1523637.1"
                     /translation="MKYKDLNENIIKLIGGKENIKSVAHCVTRLRLTLNDRSIAQTEE
                     IKKLKGVIDVVSNDVAYQIIIGTHVVDVYNEFMSMIGLSSSESSVKGEGKKEIRGIKG
                     IFNSIFVVVSETMTSVIEVLLAAGILASILALLNMSGILQSDSPTYQILDTLRSATFH
                     FLPVLIAIASAKRLNVNPYIAVVLAVTLLSSSIDGVEGLSLFGINLQVTTYANSFIPI
                     LLGVWSMGIIIGGLKKILPKALHYFLVPVLSLIITLSVTLTIFGPIGMWIGDGLNYAC
                     MFLIDTLGNWSVVALYAALQPLLIVFGAANFTMPISLNSVTTLGYDPIFMGAATISDI
                     AVCGAMFGYFLKTRNKEQKQLFGTVSFSALMGVTEPSVFGVFMKYRRPFVAVMIGGGI
                     GGLIAGLAQVKTYGMVWGLAALPTYLVGGEVSNFTFMIIGVVVSFAAATVASYLLGIP
                     SDEKEEVKDDNEKELELIINENNSRTISIGNVAKGQVIALGEVNDKAFSSGALGKGVG
                     VIPSESKSTVISPVDGTITIVFPTKHAYGIVTDEGLEILIHIGIDTVNLDGKFFESFV
                     TQNQRVKKGDALAVFESDKIIEEGFDSTIITVVTNTSDYLDVISSNDESEELLTVIV"
     gene            3219..4673
                     /locus_tag="KRL67_002772"
     CDS             3219..4673
                     /locus_tag="KRL67_002772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HBH1523638.1"
                     /translation="MNIKDTFFWGGSIAAHQCEGSWDSDNKGPAIMDFVTKGSYEMPR
                     VITDKIEEKLDYPSHNGIDFYNRYKEDIALFKEMGFSALRISIDWSRIFPNGDDENPN
                     ELGIKYYEGLIDTLIENNIEPIVTLYHFELPMNLVHKYGSWNNRKLIDLYLKYSETVI
                     RRFDDKVKYWVTFNEMNHIDPQTEHSDIFTYMIAGIKYSEIENKVQTLANVGYNMTLA
                     GVKVAKLAREINPNNKVGCVFGLNPIYPYNCNPSNVLNAFLDNDRDYYQIDAMCNGKF
                     PQYKLKEYEKNNINIEVTDEDKKAFKEGKLDFIGLNYYFSSVSTPKENLSGDKSLFGG
                     IQNPYLEQSKWGWAIDPIGLRFTLNYIYRKYQLPILITENGLGAYDKIEEDGTINDDY
                     RIEYLSKHLSQMKSAIEEDNVECFGYLMWGPIDLVSATTGQMSKRYGFIYVDLDDEGK
                     GSFKRYKKKSFDWYKEVIKSNGEVLDSSTDIVVE"
     gene            5698..6906
                     /locus_tag="KRL67_002773"
     CDS             5698..6906
                     /locus_tag="KRL67_002773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH1523639.1"
                     /translation="MENFWNPLTCFTVCVMIYAFSEFLSKKTKGAISTLLFVCIIFLL
                     GFWTNILPKDITGASGILAAMSNFGIALLITNLGTLINLEDLCHEWKTVVIALVGIVG
                     IGVGCLTVGSMLFGREYALIAAPPIAGSTVAGIIVTTVAEAANRPELAAFVILVLSFQ
                     KFFGIPITTYCINKELKFKRDNGDFLKDESKSNFKLPSMRIFKEDLGKKSSTLVYLAK
                     LSFVAMIGNFVGLATLIPGFTPANYYLNPNIACLLVGLIFTRIGFLEKGILDKSQSNG
                     IIMFGCMLMLPGGLAKVTPTALFGMIVPTIGILVVGSVFIILFTAIVGKLLGYSPRMS
                     AAIGVTCMLAYPATQIISNEGIDAMEGTEEEKQRATDYLLPKMIIGGFVTVTIASVAF
                     ASIIGPIIFG"
     gene            6937..8316
                     /locus_tag="KRL67_002774"
     CDS             6937..8316
                     /locus_tag="KRL67_002774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HBH1523640.1"
                     /translation="MIGSISINEIDKKRFKLDDLSKKIWENPEKAFKEFKACENTANF
                     LRSEGFDVEVGVGGLATAIRASFGSGKPVIGFMGEFDALPGLNQKVSTKQEAFELGAY
                     GHGCGHNLLCTAHVGAVVGLKREMIENNLSGTIVFYACPGEEQLTGKGFMARGGVFEG
                     LDLAINFHPNKISEATIGTSTAVNSVKFHFKGKTAHAGSDPQNGRSALDAVELTNVGA
                     NYLREHVTSDVRIHYTITDGGVAPNIVPDKASVWYYTRALSREAVESTYERLVKVAKG
                     AAMMTETEVEVEFLGGCYNTLNNHVLANLVSECMNEIKQEPWTQDELDFAAELDRQSP
                     QQYKAMISKYNLPEGTHLYYGGGNVTNFNSYGSTDIGDVMHIVPTAYFFTGCTNLGAP
                     GHSWQFTSCAGSSIGEKGMIYASKIMAMFGAKILNNPEIAKRAKEEFDKSMNGKTYKC
                     PIPDDIPTP"
     gene            8422..9612
                     /locus_tag="KRL67_002775"
     CDS             8422..9612
                     /locus_tag="KRL67_002775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HBH1523641.1"
                     /translation="MKKFFKEAQSIEEEIIRWRRYLHENPETGCNLPRTVTFVTSMLE
                     EMGYSPELTSNNGIVAVLDSENGGKTLLLRADMDALPIMEETDLPFKSKNKGASHSCG
                     HDNHTAMLLGAAKILMDNKHLLKGRVKFVFEPDEETTRGAKSMIDDGVLENPQVDAAM
                     AFHSMVGKFLNTGQVAYSRGPAMASADIFEITVEGKATHGASPEFGVDPINIMSHIQI
                     ALNTILSREKPQKEPVVLTIGMFNAGSVPNAIPNKASMSGTLRTFDQFVREKLKNRII
                     EISTGIAKSFGGIAKVEFKMGTPAVINDLDVGDEIVSYVKEVIGNENVIDFPSAMGSE
                     DFSEILLRVPGVFLWIGMGSVNQGYNFGMHNPKAIFNEEGLVYGSALFAHCAVKWLEN
                     NFSR"
     gene            complement(9740..11068)
                     /locus_tag="KRL67_002776"
     CDS             complement(9740..11068)
                     /locus_tag="KRL67_002776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH1523642.1"
                     /translation="MMKIAVITTEFLKEFVDNSIKKLNINAEIEIYIYRDFSHVGDLY
                     LEIEDRFDGFAVSGPIPKKAITKKAGTIKKPLVDFGTDLQSYYDMFLKLIFKYNTLDF
                     SRAYFDLTDWADNPKDISYYLSNGTFGDLMDSIDENASKMSLSDIAKLEKKIAKKHIT
                     LWNEGKIDFSTTRFSSIVPMLQNAGVNFHFIYPDIDLLKNTFNNLIKDIHLHRMKGNQ
                     PSVIYIKVKGTNYDDSILEQVYKILNSIKKDKLTDFIIEKNSNSFKIFTKCETIKSLT
                     KNFTTCYIKESIENSTNIKVCVGYGIGNDIKQAMSSAISANKESKINPLNYSFLVNEN
                     YETIGPLLCDKLLTVSNKVTPHINTIAKSSKLSTLTIQKIISVTEILQRDEVTPKELS
                     SCLNVTVRTANRFLSSLLKSGNATILYEKQNSTKGRPERVYKLFISENKY"
BASE COUNT         4138 a         1255 c         1989 g         3733 t
ORIGIN      
        1 aaactaaatt tgaagcttta cctattaata tatcttaata gtgattaaat aaaactaaac
       61 ttaagatttt acatgttaat atgtcttaag aatttattta ttaacataca attcattata
      121 tttaaaaata ttactatgat aattcataat agtaaaacag ataatggttc agacaatatt
      181 gtaaatatta gtgacagtaa aatagtatca atttagtata tgaactttgc cagataatcg
      241 cataacagag cttgtagcta tagaataaaa aaatatctag gaaaccgttg acagatttac
      301 atggatagta taacatataa aatatgaaat taaacaaaaa aaattttaat tatggcgacg
      361 tagtcataat tttgagtatg ttactgatta tcaggcagaa ctcaagtata tatgtgaaaa
      421 atagagggat actctataca tatatgcttg agttcttttt attgaaagga ggaatatatg
      481 aaaataattc agattttaaa taataatgta gccttagtaa aaagaggtaa aaacgaaatt
      541 attgtatttg tcaaaggaat tggttttaaa aaaagagtgg gacaagtaat aaatgaaaat
      601 gaaatagaga gaatatatat actagattca tacgatatgc tagaacactt tagttatcta
      661 ctacaaaact cagactccaa taatatagtt ctcattaatg aaataattga atatggtgag
      721 aaagctttaa atataaagtc aagtgattat ctaagtttaa ccctactaga ccatttagag
      781 tttttactta aaagatgtga aaagaatcaa tttataaaaa gtcctttggt atggaatgtt
      841 aaaagatttt atccaaaaca ttttgaagtt ggtatgcatg cactaaaatt aattggtgat
      901 agtaagggaa ttaatcttcc tgatgatgaa gcagtatcaa tagcacttca ttttgtaaat
      961 atggaggtaa ataaagagtc tcatgatagt acgattgtag agttaagaac tttagcagat
     1021 atagtttcta taataaaata tcattttaat gtagagctag atgaaacttc aacaaactat
     1081 atgagattta ctactcatct acagtacttt atccaaagaa ttatgtcaga tgaagcttca
     1141 atagatgaag ataattctat agatttatat aaacaagtaa gtcaacttta tccaaagtca
     1201 tttggaagtg ttcaaaagat aagaaaatat ataaaatcac aatttaatgt cgatatatct
     1261 ataagtgaag aaacttattt aatgttgcat ataaatagag tgactcaaag actggaggca
     1321 aaatcagatg aaatataaag atttaaatga aaatataata aaacttatag ggggaaaaga
     1381 aaatataaag tcggtagcac attgtgttac tcgtttaaga cttactttaa atgataggag
     1441 tatcgctcaa acagaagaaa ttaaaaaatt aaaaggagta attgatgtag tttctaatga
     1501 tgttgcttat caaattataa taggtactca tgttgtagat gtatataacg agtttatgtc
     1561 tatgataggt ttaagttcaa gtgaatcaag tgtaaaaggt gaaggaaaaa aggaaattag
     1621 aggtatcaaa ggtatattta attccatctt tgtggtagtc tcagagacta tgacttcagt
     1681 aattgaagta ctacttgcag ctggtatact tgcaagtata ttagcacttt taaatatgtc
     1741 tggcatactt caaagtgata gtcctacata tcaaatatta gatacattaa gaagcgctac
     1801 atttcacttc ttaccagtat taattgcaat agcatcagca aaaagactta atgtaaatcc
     1861 ctatatagca gtagtgttag cagttacgtt attatcttca tcaatagatg gtgtagaagg
     1921 attaagttta tttggtataa atttacaggt tacaacttat gcaaacagct ttattccaat
     1981 acttttagga gtatggtcaa tgggaattat aattggaggt ttgaaaaaaa tattgcctaa
     2041 agcactacac tattttttag tcccagtatt atctttaata ataactttat cagttacatt
     2101 aactatattt gggcctattg gtatgtggat tggtgatgga ttaaactatg catgtatgtt
     2161 tttaattgat acattgggta attggagtgt tgttgcactg tatgcagctt tacaaccact
     2221 cttaatagtt tttggtgcag caaactttac tatgcctata tctttaaatt ctgtgacaac
     2281 attggggtat gacccaattt ttatgggcgc tgcaacaata tctgatattg cagtttgtgg
     2341 tgcaatgttt ggatatttct taaaaactag gaataaagaa caaaagcaat tatttggtac
     2401 agtaagcttt agtgcactta tgggagttac agagccttca gtgtttggtg tgtttatgaa
     2461 atatagaaga ccatttgtag cagtaatgat aggtggaggt ataggtggtt taatagcagg
     2521 tttagcacaa gttaaaacat atggaatggt ttggggatta gctgcattac caacatatct
     2581 tgttggagga gaggtatcta actttacatt tatgataata ggtgtagtag tatcatttgc
     2641 agcagcaaca gtagcttctt atttactagg aataccttca gatgaaaaag aagaagttaa
     2701 ggatgataat gaaaaagaat tagaactaat aatcaacgag aataattcaa gaactattag
     2761 tattggaaat gttgcaaaag gtcaggtaat tgcacttggg gaagttaatg ataaagcatt
     2821 ttcctctgga gcattaggta aaggggttgg agttatacct agtgaatcaa aatcaactgt
     2881 tatatctcct gtagatggaa ctataactat agtatttcca actaaacatg catatggtat
     2941 agtgactgat gaaggattag aaatattaat tcatattggg atagatactg taaacctaga
     3001 tggaaagttc tttgaaagtt ttgtaacaca aaatcaaagg gttaaaaaag gagatgcttt
     3061 agcagtattt gaatcagata aaattataga ggaaggtttt gattcaacta taataactgt
     3121 agttacaaat actagcgatt atttagatgt aatctcatca aatgatgaat cagaagaatt
     3181 attaactgta atagtataaa ttttaggagg ggaaattcat gaatattaaa gatacatttt
     3241 tctggggagg aagtattgca gcccatcaat gtgaaggttc atgggatagt gataacaaag
     3301 ggcctgcaat tatggatttt gtaactaagg gctcttatga aatgccaaga gtaattactg
     3361 ataaaataga agaaaaattg gattatccat ctcataatgg aatagatttt tataatagat
     3421 acaaagaaga tatagcttta tttaaagaaa tgggatttag tgcattaaga atatctatag
     3481 attggtcaag aatttttcca aatggagatg atgaaaatcc taatgaacta gggattaaat
     3541 actatgaagg tctgatagat acattaatag aaaataatat agaacctatt gtaacactat
     3601 atcattttga attacctatg aatttagtac ataaatatgg ttcatggaat aacagaaaac
     3661 ttatagattt gtatttaaag tacagtgaga ctgtaataag aaggtttgat gataaggtaa
     3721 agtactgggt tacatttaat gagatgaacc atattgaccc tcaaacagag cattcggata
     3781 tttttactta tatgatagca ggtattaagt atagtgaaat agaaaataag gttcaaacat
     3841 tggcaaatgt agggtataat atgactttgg cgggtgtaaa ggttgcaaag ttagctagag
     3901 aaatcaatcc caacaataaa gttggatgtg tatttggact aaatccaatt tatccataca
     3961 attgtaatcc tagtaatgtg ttaaatgcat ttttagataa tgaccgtgat tattatcaaa
     4021 ttgatgcaat gtgcaatggg aagtttcccc aatataaatt aaaagaatat gaaaaaaata
     4081 atataaatat tgaagtgact gatgaagaca aaaaagcatt taaagaaggt aagttagatt
     4141 ttataggtct taattattat ttttcaagtg tatctactcc aaaagaaaat ttatcaggtg
     4201 ataaatcttt atttgggggg attcaaaatc catatttaga acaaagtaag tggggatggg
     4261 caatagaccc tataggttta agatttactt tgaactatat ttacagaaag tatcaattac
     4321 ctatcttaat tacagaaaat ggtttagggg catatgataa aatcgaagaa gatggaacta
     4381 taaatgatga ttatagaatt gaatatttaa gtaagcatct atctcaaatg aagtctgcaa
     4441 ttgaagagga taatgttgaa tgttttggat atttgatgtg gggaccaatt gatttagtca
     4501 gtgcaactac aggtcagatg agtaagagat atggatttat atatgttgac ttagatgatg
     4561 aaggtaaagg aagttttaag agatataaga aaaaatcgtt tgattggtac aaagaggtaa
     4621 taaaatctaa tggagaagtt ttagattctt ctacagatat agtagtagag taggaataag
     4681 ataaatagaa gttagaaact gatgttaatt agtgtctagt aaagcgttta aaatagagta
     4741 tagggttttt aaaataaagt gtgtattctt cttaaaagta atgataacta aaattactat
     4801 acaaaggaga atacacattt tttacgttta tacttaagta aatatttaga catattcata
     4861 atatgattta ctcattatat aaaaatagta tgatttactc actagataaa tttataaatt
     4921 ttctggaaaa atgtcagaat atattgacaa agaaaaggtt tgaaattata atctatatta
     4981 agaatagttt gacaatataa aaacaagtat ttttacaaat agtgaaatta tttttgttgt
     5041 aaatttaaga ataatttagg actactatag agataaaggt atagatgtca ttatatgata
     5101 aattatactt gtatagaaac aattttaaag tatggcttat tattgattta atataataaa
     5161 taatttttat ttctagtaat agttcgtagg aataagatat ctaaatttgc ttagaaatag
     5221 tctaatttgc aaaggagggg atgcactaat agataaaatt tttacataaa gaagacttaa
     5281 agtgaagtat attctgataa tattggttta atgggtatat tattattttg ttttagatat
     5341 tttaagaata atttaagacg tttgtaaaaa tgtattctat atctaatatt taaataaata
     5401 taagtcttcc aacatgtata ctaagataga aagtagttga agtacaattc taacttgtaa
     5461 aattatgtag aaagtaaaaa tcaaaaagaa atctagtaag ttgataacta cagttaatat
     5521 ttagagagtt aactctagtt atatttattt agatatttta agaataattt aagacgtata
     5581 taatatcatc caaaaatatc aagctatacc atatgtagtt aagaataact gcataatata
     5641 cattttataa ttattgtttt attgataaaa aattattata tttaggagga aacaacaatg
     5701 gaaaattttt ggaatccact tacttgcttt actgtatgtg taatgattta tgcttttagt
     5761 gagtttttat ctaagaaaac aaaaggtgca atttcaacat tgctatttgt atgtattata
     5821 tttttactag gattttggac aaatatttta ccaaaagaca ttacaggagc atcaggaata
     5881 ttagctgcaa tgagcaactt tggtatagct ttattaataa ctaacttagg aacacttata
     5941 aatcttgaag acttatgtca tgaatggaaa acggttgtaa ttgcattagt aggtattgta
     6001 ggaatagggg ttggctgttt aacagtgggt agtatgttat ttggtaggga gtatgcttta
     6061 atagcagcac ctccaatagc gggttctact gttgctggga ttatcgtaac aactgttgca
     6121 gaagctgcaa atagacctga acttgctgca tttgtaattc tagtattatc atttcaaaag
     6181 ttctttggca ttccgataac tacatattgt ataaataaag aattaaaatt taaaagagat
     6241 aatggggatt ttttgaaaga tgagtcaaaa agtaatttta aacttccaag tatgagaatt
     6301 tttaaagaag atttaggtaa aaaatcatct actttagtgt atcttgcaaa gctttcattt
     6361 gttgctatga ttggtaattt tgtaggattg gctactttaa taccaggatt tacccctgca
     6421 aactattatt taaatcccaa catagcatgt ttgttagtag ggttgatttt tacacgaata
     6481 ggttttttag aaaaaggaat tttagataaa tctcaatcta atggaattat aatgtttggc
     6541 tgtatgttaa tgttacctgg aggacttgct aaggtaactc caacagcact gtttgggatg
     6601 attgttccaa ctataggaat acttgttgta ggctctgtat ttataatact atttactgca
     6661 attgtaggaa aactattagg atattctcca agaatgtcag ctgcaattgg tgtaacttgt
     6721 atgctagctt atcctgcaac acagataatt tctaatgaag gtattgatgc tatggaagga
     6781 actgaagaag aaaaacaaag ggcaacagat tatttattac caaagatgat tataggagga
     6841 tttgttactg taaccattgc atcagtggca tttgctagta taataggtcc aattatattt
     6901 ggttgatagt aaatagactt ataggaggaa taggaaatga taggaagtat atctataaat
     6961 gagatagata aaaaaagatt caaacttgat gatttaagta agaaaatatg ggaaaatcca
     7021 gaaaaagcat ttaaagaatt taaagcatgt gaaaatacag caaactttct aagaagtgaa
     7081 ggatttgatg tggaagtagg tgtaggaggt ttagccacgg ctattagagc tagttttggt
     7141 tcaggcaaac cagttatagg ttttatggga gaatttgatg cattacctgg tttaaatcaa
     7201 aaagtttcaa caaagcaaga agcatttgaa ttgggtgcat atggccatgg atgtgggcat
     7261 aatttattat gtacagcaca tgtaggggca gtagtagggc ttaaaagaga gatgatagaa
     7321 aataacttaa gtgggactat agtattttat gcatgtccag gagaagaaca attaacaggt
     7381 aaaggattta tggctagagg tggagtattt gaagggttag acttagctat aaattttcat
     7441 ccaaataaaa ttagtgaagc tacaattggt acttcaacag ctgttaattc tgtaaaattt
     7501 cattttaagg gaaagactgc acatgcaggt tcagaccctc aaaatggaag aagtgcacta
     7561 gatgctgtag aacttactaa tgtgggagct aattatttga gagaacatgt tacttctgat
     7621 gtaaggattc attatacaat tacagatgga ggagtagcac ccaacatagt tcctgataaa
     7681 gcatcagtat ggtattatac aagagcttta agtagagaag ctgtagaaag tacatatgaa
     7741 agacttgtaa aggtagcaaa aggagctgca atgatgactg aaactgaagt tgaagtagag
     7801 tttttaggag gttgttataa tactttaaat aatcatgttt tagcaaatct tgtatcagaa
     7861 tgtatgaatg agattaaaca agagccttgg actcaagacg aattggattt tgcagctgaa
     7921 ttagatagac aaagtcccca acaatataag gctatgataa gtaaatataa tttgccagaa
     7981 ggaactcatc tatattatgg tggaggaaat gttacaaact ttaatagtta tggctctact
     8041 gatattggag atgtaatgca tatagttccg acagcatact tttttacagg gtgtacgaat
     8101 ctaggagcac caggtcatag ctggcagttt acatcttgtg caggaagctc tataggagaa
     8161 aaaggtatga tatatgcatc aaaaattatg gctatgtttg gagctaagat attaaataat
     8221 ccagagatag ctaaaagggc aaaagaagaa tttgataaat ctatgaatgg taaaacatat
     8281 aaatgcccta ttccagatga tattccaact ccttaaaaag acatgaagtt taatatataa
     8341 aattataatt tttatgccag catcatttca ttactaaaag aaagtgagat tttgctggct
     8401 atctgattag gaggaaggaa tatgaaaaaa ttttttaaag aagcacagtc aatagaagag
     8461 gaaattatta gatggagaag atatttacat gaaaatcctg aaactggttg caatctaccg
     8521 agaacagtta catttgttac cagcatgtta gaagaaatgg gatattctcc agaattaaca
     8581 tcaaataatg gaatagtagc tgttttagac agtgaaaatg gtggaaagac cttactatta
     8641 agggcagata tggatgcgct tcctattatg gaggagacgg atttaccttt taaaagtaag
     8701 aataaaggtg catctcatag ctgtggacac gataatcaca cagcaatgct cttaggagca
     8761 gctaaaatac ttatggataa taagcactta cttaaaggta gagtaaagtt tgtatttgaa
     8821 ccagatgaag agaccaccag aggtgcaaaa tcaatgattg atgatggtgt attagaaaat
     8881 cctcaggtcg acgcagctat ggcatttcac agtatggtgg gcaaatttct taacacaggt
     8941 caagtagctt attcacgtgg tccagctatg gcatctgcag atatatttga aataacagta
     9001 gaaggaaaag caactcatgg agcaagccct gaatttggag tggacccaat aaacataatg
     9061 tcacatatac aaatagcatt aaacactata ctttcaagag aaaaacctca gaaagagcct
     9121 gtagttttaa ctattggtat gtttaatgca ggaagcgtac caaatgctat accaaataaa
     9181 gcatctatgt caggaacttt aagaacattt gaccaatttg ttagagaaaa attaaaaaat
     9241 agaattattg aaatatctac aggaatagct aaatcatttg gaggaattgc taaagtggag
     9301 tttaaaatgg ggactcctgc agttataaat gatttggatg taggagatga gattgtatct
     9361 tatgttaaag aagttatagg aaatgagaat gtaatagact ttccatctgc aatgggttcg
     9421 gaagattttt cagagatttt actaagggtt ccaggagtat ttttatggat tggaatggga
     9481 agtgttaatc agggatataa ttttggtatg cataatccga aggcaatttt taatgaagag
     9541 ggattagttt atggttcagc tttatttgct cattgtgcag taaaatggtt agaaaataat
     9601 tttagcagat aataacataa aatattatta aatatcataa aaggtatacc ttaaaaagat
     9661 gcaaagatat accttttaga aaatcatata taattttact taatattaga tgataaaata
     9721 tttttatgta ttttggattt taatatttat tttctgatat aaataatttg tatacccttt
     9781 caggtctacc ttttgttgag ttttgttttt catataatat agtagcgttt ccagatttta
     9841 agagactact tagaaatcta ttagcagttc taacggttac atttaaacat gaagataatt
     9901 ccttaggagt aacttcatct ctttgtagaa tttcagtaac agatataatc ttttgaatag
     9961 taagagtaga cagtttagaa cttttggcaa ttgtatttat atgtggagtt actttatttg
    10021 aaacagtaag taacttatca catagtaaag gtcctattgt ctcataattt tcgttaacta
    10081 agaaactgta gtttaaagga tttattttag attccttatt agcacttata gcactagaca
    10141 tagcttgctt tatatcatta ccaattccat atccaacaca tactttaata tttgtactat
    10201 tttcaataga ttcttttata taacatgtag taaaattctt agttaatgat ttaatcgttt
    10261 cacatttagt gaaaatttta aaagaattac tatttttttc aattataaaa tcggttaatt
    10321 tatctttttt aatactattt aatattttat aaacttgctc taaaatagaa tcatcataat
    10381 ttgtaccttt aacttttata tatattacac ttggttgatt tcccttcatt ctatgtaaat
    10441 gaatatcttt tattaagtta ttaaaagtat tttttaataa atctatatca gggtatatga
    10501 aatgaaagtt tactcctgca ttttgtaaca taggaacaat actactaaaa cgagtagtag
    10561 aaaaatcaat tttcccttca ttccatagag taatatgttt ttttgcaatc tttttttcaa
    10621 gttttgctat gtcagataaa ctcattttag atgcattttc atcaatacta tccattaagt
    10681 ctccaaatgt gccatttgat aaataatatg atatatcttt tggattatct gcccagtctg
    10741 ttaagtcaaa gtatgctcta gaaaaatcta atgtattata tttaaaaata agctttagaa
    10801 acatatcata gtaactttgc aaatcagtac caaaatcaac tagaggtttt ttaatagttc
    10861 cagccttttt tgtaatagct tttttgggga tagggccact aactgcaaat ccatcaaacc
    10921 tatcttctat ttctaaatat aaatctccaa catgagaaaa atctctatag atatatattt
    10981 caatttctgc attaatattt aactttttta tagaattatc tacaaactct tttaaaaatt
    11041 cggttgttat gactgctatt ttcatcatag attacttcct tttcaattat tataattatt
    11101 ttatattaaa tatta
//
LOCUS       DAEPXF010000127        11044 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain HB27
            SAMN13376389-rid12479423.denovo.134, whole genome shotgun sequence.
ACCESSION   DAEPXF010000127 DAEPXF010000000
VERSION     DAEPXF010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13376389
            Sequence Read Archive: SRR10518432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 11044)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11044)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001069624.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001069624.1
            Genome Coverage       :: 315x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/15/2021 01:54:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,165
            CDSs (total)                      :: 4,076
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 89
            rRNAs                             :: 10, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 10 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 19 of 69
            Pseudo Genes (incomplete)         :: 44 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 10 of 69
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11044
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13376389-rid12479423.denovo.134"
                     /strain="HB27"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="China: Hebei"
                     /collection_date="2017"
     gene            120..1151
                     /locus_tag="KRL67_002777"
     CDS             120..1151
                     /locus_tag="KRL67_002777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HBH1523643.1"
                     /translation="MENICDMNNKVKVAVLDTGIDKEHDYLKDNLVGGIAFECMHDYI
                     FISDKFDDEDGHGTACASIIKKEYEDVELFVIKILRKDGITNIKVLEEALRYLLDTNI
                     RLINLSLSVIGVESVKGLFEVCYELFRKGKIIVCSLANDFDLSYPAMFNNVIGVRGFT
                     LDGDDSFWYNKKYDVQCVMDSNSYISCDINNSYRLPPKCNSYLSAKLTGKIAKILSEK
                     PGITISDLNDKLESLATRNHWNSCDFDECSRIVNFKLDLYDKENALLMKVADVVRDCL
                     DIEDNNEKLFKGSLFNKEIGLVYDNCFNLLKKLEHRFDIKFNYMDISKYDLVSIYTLT
                     ELVERYKNL"
     gene            1284..2582
                     /locus_tag="KRL67_002778"
     CDS             1284..2582
                     /locus_tag="KRL67_002778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional folylpolyglutamate
                     synthase/dihydrofolate synthase"
                     /protein_id="HBH1523644.1"
                     /translation="MKYEEALEYISQTNKFGIRLGLENIGKLLELLGNPQETLNIIHV
                     AGTNGKGSVCSFVSNILIECGYKVGLYTSPYLETFTERIRVNGQNIPQDDVVRIIELI
                     KEKIEIMVREGYAYPTEFEVVTAMAFYYYSEQKVDFVALEVGLGGRYDATNIITKSLV
                     SVIVSISLDHTGILGDTIEKIAYEKAGIIKENGVVLVYDQTDEAKDVIKSVCKEKKAK
                     YIEVDFDDINIKKSDINSQIYDCTVMKETYRDLEIKLIGEHQINNSILAISVIKYLKD
                     INKLANINEESIRKGLINTKWPGRIEKIKENPIFIIDGAHNEDGAKSLAKALDKNFKG
                     RKLTLLIGMLEDKDIDSVLEILLPHFNKVITTTPSNPRAINSDILREKVLKYVDDVTS
                     KHEIEDAVNYTLETSSEDDIIISAGSLYMIGTVRTLVKKL"
     gene            complement(2696..2941)
                     /locus_tag="KRL67_002779"
     CDS             complement(2696..2941)
                     /locus_tag="KRL67_002779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03905 family TSCPD domain-containing
                     protein"
                     /protein_id="HBH1523645.1"
                     /translation="MKVTFNPSGVCCREMIFEVDENNVIVDAEFIGGCNGNLSGLRSL
                     IIGQNALEVADKLNGIDCGGKGTSCPDQLSKAIRQSI"
     gene            3089..3955
                     /locus_tag="KRL67_002780"
     CDS             3089..3955
                     /locus_tag="KRL67_002780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YncE family protein"
                     /protein_id="HBH1523646.1"
                     /translation="MKIYISNYLSKSISIVDYSTLELEKEIVLEDNIYPHHFCIEKEK
                     NLIYIPSSSNGILYVLDLSNDKIIDTVSIGGSLSQVVLSDNELFVANEDSNSIYVLDK
                     NTLNPIGIIGVDNMPHGFDFDRESKKLYVPCINSIVCIDTINKNIQKKINMDFRAWHI
                     ELDKQKKEMYISTLDGKLIIIDEVSMDIKKVLYEFLLPVEIRFNYSGKKVYVADLGYN
                     NVRILDYTTSRYIGNIEINGIPQGLEISKDEKLLFVSDTQENSVKVYETANNKLIKEI
                     KVGKEPTTIICL"
     gene            complement(3959..4495)
                     /locus_tag="KRL67_002781"
     CDS             complement(3959..4495)
                     /locus_tag="KRL67_002781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4364 family protein"
                     /protein_id="HBH1523647.1"
                     /translation="MFENSSEELAYHKLLILYILEKIKMDLTNSQITQVVLETEMMNY
                     FSLQQLLSQLMESKFLTTYKDSDREYYALTQRGVESLEYFFNRIPTSVTEKIDKYIDS
                     NKENLLADTQVKSSFVKQSDNEFIVNLRVIENQSNLIDLNLNVSSEKQAKLICNNWKN
                     NASYMYAEVIDLLIRDIH"
     gene            complement(4686..4889)
                     /locus_tag="KRL67_002782"
     CDS             complement(4686..4889)
                     /locus_tag="KRL67_002782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta-type small acid-soluble spore
                     protein"
                     /protein_id="HBH1523648.1"
                     /translation="MSNSNRTVVPEAKAALNQMKLEIANEIGLSNYENIDKGNLTARE
                     NGYVGGYMTKKLVEMAERQMAGK"
     gene            5180..5944
                     /locus_tag="KRL67_002783"
     CDS             5180..5944
                     /locus_tag="KRL67_002783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HBH1523649.1"
                     /translation="MKREVKRTKALIYSVLFFSLLLVLIFVFVYGIKYICSKSDNSKM
                     PIYKVDTNEKKIALSFDVAWGTDNMDDILKILDKHNVKATFFLVGSWVDDNEEIVKAI
                     DEKGHEIGNHSNTHANLKEISKEDITKEIETTSEKIYNITGKKTNLFRPPFGDVNNKA
                     MDICSDLGYKVIKWDVDSIDWKELGPNHVIEKVIKESQPGSIVLFHANINDVDNYLDT
                     IISRLKKDGYSLVKISDLLYKDNYVVDSNGVQKIKN"
     gene            6333..7646
                     /gene="prdC"
                     /locus_tag="KRL67_002784"
     CDS             6333..7646
                     /gene="prdC"
                     /locus_tag="KRL67_002784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline reductase-associated electron transfer
                     protein PrdC"
                     /protein_id="HBH1523650.1"
                     /translation="MNKLYSILLKQHVGGPDKPVVSVGDVVKKGTLIAEPTGLGANIY
                     ASVSGKISEINDQAIVIEADEVQEDTFEPLKGEGILDLIKEAGVVGMGGAGFPTHVKL
                     NIDLNGGTILANAAECEPLLAHNIKEIEERPEIVYQGIKYAMEVTNAGKGMLAIKSKH
                     PKAIEAFKKVIKPGDNIEVAELVDMYPMGEERAIVRDVLGKLLEPTQLPSEANAVVIN
                     VETLTRIVEAVEQKRPVISKNITVVGQLNSGKESIVFEDVPIGTTVGELIERAGGIKG
                     EYGEIILGGPFTGKATTLDAPITKTSGGIIVTMPFVNEKRKMGLLVCACGPNEERMRD
                     IATKMGVTDIVSVQKCKQAQEIKGALKCENPGHCPGQAQKCIEFKKAGAEVILIGNCT
                     DCSNTVMGSAPKLKLGVYHITDHVMRTVNHPLIRRIKGDVRLKIK"
     regulatory      8050..8135
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01786"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class II; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01786"
     gene            8717..10834
                     /locus_tag="KRL67_002785"
     CDS             8717..10834
                     /locus_tag="KRL67_002785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-Gln thioester bond-forming surface protein"
                     /protein_id="HBH1523651.1"
                     /translation="MKTKIKKSSIISLVIAFSMVFTTFTPIVSYADEVTSNDTILNGE
                     EQSNGQTPDVEKPSDGQVPDGEKPSDGQIPDGEKPSDGQIPDGEKPSDGQIPDGEKPS
                     DGQIPDGEKPSDGQMPDGEKPSDGQIPDEKKPLDEQTPEEEKPLEEEIVIEEMSLKQD
                     IDKILDMTISQINKIVYNFWEDEENVKADEQSEINQVFTSEDSFISLWYDKKAKVKNS
                     CLLKEIDGSDRPYHDLRFDDITKTLTFDYVINGLVGASSKDNKYVIEGEDGEQTAFCY
                     NNHLRPPSSDGKSPYLPAEEFSGQENQNEDAVKSILYAGSEFDGFGYKQQFNLCEEEN
                     DIMTYSATQSAIWIILGQIDEQERLDQYQGSINLCDKLIERAKTEEERAEYQKKKEVA
                     INTKAYLEALLKAGREELKPNDTGKPSLSNGLTKINFEKNEDGTYETEAVALVGYSGV
                     VKLILPDGVTAYDGDGNIIGTGEVEISTQQKFKLKSVGKPDAKANISAVSYDYIFPKA
                     IQYYKAVLDLGQKDHSSSLPSSKQNLLSYTIEKKNGQEVNFNIGLPTDDDNVVNPPVP
                     PIDDDVVNPPVPPTDDTNGHKPKPSPPIDDTVINPPVPPTDDTVINPPVPPTDDAVLN
                     PPVPPTDDAVLNPPVPPTDDAVLNPPVPPVSDTVEKMPELSRDDTIVKSPKTGDETQL
                     MSYVFISVIAICGLVYQCKIKRN"
BASE COUNT         4462 a         1236 c         1911 g         3435 t
ORIGIN      
        1 tatatggtgt acttatttta tgtttttagc aagaaatata gcatttaaaa gataaattgt
       61 gcaacttaaa tcaaagttta agatgaacta ttaacataga tatattaaaa acttttgtaa
      121 tggagaatat atgtgatatg aataataaag ttaaggttgc agtattagat acaggaatag
      181 acaaagaaca tgattactta aaagataatt tagttggagg aattgctttt gaatgtatgc
      241 atgattatat ctttatatca gataaatttg atgatgaaga tggtcatgga actgcttgtg
      301 catcaataat aaagaaagag tatgaagatg tagagttatt tgtaataaaa attttgagaa
      361 aagatggtat tacaaatata aaagttcttg aagaagcatt aagatacctt ttagacacta
      421 acataagatt aattaattta agtttatcag tcataggagt agaaagtgtt aagggtttat
      481 ttgaagtttg ctatgaattg tttagaaaag gaaaaataat agtatgttca ctcgctaatg
      541 attttgactt aagttatcca gctatgttta acaatgttat aggtgtaagg ggatttactt
      601 tagatggaga tgattcattt tggtacaaca aaaaatatga tgttcaatgt gttatggata
      661 gcaattcata tataagttgt gacataaata attcctatag gttaccacca aaatgtaata
      721 gttatttatc agcaaagctt actggtaaaa tagctaagat attatcagaa aaaccaggca
      781 taacaatatc tgatttaaat gataagcttg aatcattagc aactagaaat cattggaata
      841 gttgtgattt tgatgagtgt agtaggattg taaactttaa attggatttg tatgataaag
      901 aaaatgctct tcttatgaaa gttgctgatg ttgttaggga ttgtttagat attgaagata
      961 ataatgaaaa actctttaag ggtagccttt ttaataagga gataggcttg gtttatgata
     1021 attgttttaa tttgttgaaa aagctagagc atagatttga cataaagttt aattatatgg
     1081 acatctcaaa atatgactta gtttcaatat atacactgac tgaattagtg gaaagataca
     1141 aaaacttata aataaagatt tgtagctaaa tttaatgtat aaggtattaa attatatgta
     1201 ttctttgtca gtagattaaa agtaaaaaat atactataat tagataacat aagagaaaat
     1261 gccaatacag gaggaattag ataatgaaat atgaagaagc attagagtac atatcacaaa
     1321 ctaataaatt cggtataaga ttaggattag aaaatatcgg aaagttatta gaattattag
     1381 gaaatccaca agaaacttta aatataatac atgtagctgg aactaatgga aaaggttctg
     1441 tgtgttcttt tgtttctaat atactaatag agtgtggata taaagttggt ttatatacat
     1501 caccatattt agaaactttt actgagcgta taagagtaaa tggacaaaat ataccacaag
     1561 atgatgttgt tagaataatt gagttaataa aagaaaaaat agagattatg gtaagagaag
     1621 gctatgcata tccaacagag tttgaagtgg ttactgcaat ggcattttat tactattcag
     1681 aacaaaaggt agattttgtg gcattagaag ttggtttggg tggcagatat gatgctacaa
     1741 atataataac aaaatcactt gtaagtgtga tagtatctat aagtttggac catacaggga
     1801 tacttggaga tacaatagaa aaaatagcgt atgaaaaggc tggaataata aaggaaaatg
     1861 gagtagtact agtatatgac cagactgatg aagcaaagga tgttataaag tcagtttgta
     1921 aagagaaaaa agctaaatat atagaagttg attttgatga tataaacata aaaaaatcag
     1981 atataaattc tcaaatatac gattgtactg taatgaaaga aacttataga gaccttgaaa
     2041 taaaattgat aggcgaacat caaataaaca attctatctt ggcaattagt gtaataaaat
     2101 atctaaaaga tataaataaa ttagcaaata taaatgaaga atctataaga aaaggactta
     2161 taaatactaa atggccagga agaatagaaa agataaaaga aaatccaatt tttataatag
     2221 atggtgcaca taatgaagat ggagcaaaat ctttggcaaa agcacttgat aagaatttta
     2281 aaggtagaaa attaacctta ttaataggta tgttagaaga taaagatatt gatagtgttt
     2341 tagagatatt gctgccacat tttaataagg taataacaac aacaccaagt aacccaaggg
     2401 ccataaattc agatatatta agagaaaaag tattaaaata tgtagatgat gtaacatcaa
     2461 agcatgagat agaggatgca gtaaattata cattagaaac atcaagtgag gatgatatta
     2521 taataagtgc aggttcatta tatatgattg gtacagtgag aactttagta aagaaattat
     2581 aattttataa aataaatata caaataaaaa tataaaaaag agttgtctaa aaaatggaca
     2641 actcttttta tataaagtaa gtttttaata attttactga tgtttatata agtaattata
     2701 tagattgacg tatagcttta gataattggt ctgggcaaga agtacctttt ccaccacagt
     2761 caataccgtt taatttatca gcaacttcaa gagcattttg gccaattatt aagcttctaa
     2821 gtcctgataa attaccatta catcctccta taaactcagc atcaacaatc acattatttt
     2881 catcaacttc aaaaatcatt tctctacaac atacaccact tggattaaat gttactttca
     2941 ttaaaaagac ctccattaaa tattatttct ttttttaaca ttaataataa taccaaatat
     3001 atgcgtaata atgcaactat aaagcataat acaagctaat gaaataatat attattgtag
     3061 tatatgtatt gtagagagga ggggttatat gaaaatctat atttctaact acttatcaaa
     3121 gagtattagc atagttgatt attctacact tgaactagaa aaagaaatag tattagaaga
     3181 taatatatat ccacaccatt tttgcataga aaaagaaaaa aatttaatat acatacctag
     3241 ttcaagtaat gggatactct atgtcttaga tttaagtaat gataaaataa ttgatactgt
     3301 ctctatagga ggaagtctta gtcaagttgt acttagcgat aatgaattgt ttgtggcaaa
     3361 tgaagattct aatagtatat atgtactaga taaaaacaca ttaaatccaa taggaattat
     3421 tggagttgat aatatgccac atggatttga ctttgataga gaaagcaaaa agttatatgt
     3481 accgtgtata aactcgatag tatgtataga tacaattaat aaaaatatac aaaagaaaat
     3541 aaatatggat tttagagctt ggcatataga gcttgataaa caaaagaaag aaatgtatat
     3601 atcaacactt gatggaaagc ttataataat agatgaagta agcatggata taaaaaaggt
     3661 attatatgaa tttttattac ccgtagaaat aagatttaat tatagtggca aaaaagttta
     3721 tgttgctgat ttgggttata ataatgtaag aattttagat tatacaacga gtagatacat
     3781 tggaaatatt gaaataaatg ggattcccca aggattagaa atatctaaag acgaaaaatt
     3841 attatttgta tcagatacac aagaaaattc agtaaaagtt tatgaaactg caaataacaa
     3901 attaataaaa gaaatcaagg taggaaaaga gcctacaact ataatttgtt tgtaggcttt
     3961 aatgtatgtc tcttataagt aaatcgataa cttcggcata catataagat gcattatttt
     4021 tccagttatt gcaaataagt tttgcctgtt tctctgatga aacatttaga tttaaatcta
     4081 ttaagttaga ctgattttct atgactctaa ggttgacaat aaattcatta tcactttgtt
     4141 ttacaaaact agactttact tgagtatctg ctagtagatt ttctttgtta gaatctatat
     4201 acttgtctat tttttcagta acactagtag gtattcgatt aaagaagtat tctagacttt
     4261 ctacgcctct ttgagtaaga gcataatatt ctctgtcaga gtctttatat gtagtcaaaa
     4321 acttagattc cataagctga gataaaagct gttgtaatga aaaataattc atcatctcag
     4381 tctctaatac tacttgagtt atttgagaat tagttaaatc cattttaatt ttttctaata
     4441 tatataaaat taaaagtttg tgataggcta attcttcaga cgagttttca aacataaata
     4501 tcatcctccg tcaaactatt tctatatata atatattaaa ctaatttgta caaaattaaa
     4561 agaacaattt gtattgtatt attaatcttt agtaattcat ataaaaacga ttttataaat
     4621 aaatatagaa aaacagggct gctaattagc agccctgttg atacccaggt gtcaaaattt
     4681 actatttatt ttccagccat ttgtctttca gccatttcaa ctaatttctt agtcatatat
     4741 ccaccaacat atccattttc tctagctgtt aagttacctt tgtctatgtt ttcatagtta
     4801 gataaaccta tttcgttagc tatttctaat ttcatttggt ttaaagcagc ttttgcttct
     4861 ggaactactg ttctgtttga atttgacata gtaaatttcc tcctcaaata attagtttat
     4921 gatgtctaac aacactttta tttatgtgat gttaatacta atttctccaa aataaaaata
     4981 atatatacag aaaaataaag gtaaaaatgt aaaaaatata aaaaaattaa ataaatgagt
     5041 attttagaga gtttaattag gtcaatatag gttttatttt tttagttatt gtcttataaa
     5101 ttaaatatta cagtactttt ttgagctgaa aaaaaataaa ttttaaatat agaagaaatt
     5161 atttcaaagg gggaatagta tgaaaagaga agttaaaagg actaaagctt tgatatatag
     5221 tgttttattt ttttcattat tattagtgtt aatatttgtt tttgtttatg gaataaaata
     5281 catatgttct aaatctgaca attcaaaaat gcctatatat aaagttgata caaatgagaa
     5341 aaaaatagct ttgagttttg atgtagcatg gggaactgat aatatggatg atattttaaa
     5401 aattttagat aaacataatg taaaagctac cttcttttta gttggtagtt gggtggatga
     5461 taatgaagaa attgtaaaag ccatagatga aaaaggacat gaaattggaa atcattcaaa
     5521 tactcatgcc aatttgaagg aaatatcaaa agaagacata acaaaagaga tagaaactac
     5581 atcagaaaaa atatataata taacagggaa aaagactaat ttatttagac ctccttttgg
     5641 ggacgtaaac aataaagcta tggacatttg tagtgattta ggttataaag taattaagtg
     5701 ggatgtagat tctatagatt ggaaggagct tggaccaaat catgtaattg aaaaagtaat
     5761 aaaagaatct caacctggtt caatagtttt attccatgct aatataaatg atgtagataa
     5821 ctatttggat actataataa gtcgactaaa aaaagatgga tacagtctag tcaaaatatc
     5881 tgatttgttg tataaagata attatgtagt tgattccaat ggagtgcaaa aaattaagaa
     5941 ttaataagta ataaatgtat attttaagta aagttgtggt aaattatata gtatatgtta
     6001 gaaaagtgtc gataaaaaga cggtgtctat tttttgacac tttttataca tgtatgcaat
     6061 atcaataatt atagattaaa ttgattaaaa aagtgtctaa taattgactt ttttttgatg
     6121 ggtttaaaaa tttagaagaa ttaatgaatg ctgtaagaca gtatttccaa agggtataag
     6181 tttgtttaaa agttggcata gaaattgctt tgttaataag cgatatgttc tatagaaata
     6241 aacaatatgt taagacctta taatagaaaa atagcattaa cattatttga acagcttata
     6301 aaaaaattaa tatacttaag ggggcgtact agttgaacaa actatattct attttattaa
     6361 aacaacacgt tggtggtcca gataagccag tagtaagtgt tggagatgta gtaaaaaaag
     6421 gaactctaat cgcagaacca actggattag gggcaaatat ttatgccagt gttagtggaa
     6481 aaataagtga aataaatgac caagcgatag taattgaagc tgatgaagta caagaagata
     6541 cttttgaacc attaaaagga gaaggtatac ttgacttaat taaagaagct ggagtagtag
     6601 gaatgggtgg agcaggattc cctactcatg taaaattaaa catagattta aatggtggaa
     6661 caatattagc aaatgcagct gaatgtgaac ctttattagc tcataatata aaagaaatag
     6721 aagaaagacc agaaatagtt tatcaaggta taaaatatgc tatggaagtt actaatgctg
     6781 gaaaaggtat gctagcaata aaaagcaaac atccaaaagc aatagaagct ttcaaaaaag
     6841 taataaagcc aggagacaac atagaagttg cagaactagt tgatatgtac ccaatgggag
     6901 aagagagagc aatagtaaga gacgttctag gaaaattact tgagccaact caattacctt
     6961 cagaggctaa tgcagtagta ataaacgtag aaacactaac tagaatagta gaggctgttg
     7021 agcaaaaaag accagttata tctaaaaata taactgtagt tggtcaatta aacagtggaa
     7081 aagaatctat agtatttgaa gatgtgccaa ttggaacaac tgtcggagaa ttaattgaaa
     7141 gagctggtgg aataaaaggt gaatatggtg aaataatcct aggaggacct ttcactggta
     7201 aagctactac tttagatgct ccaataacta aaactagtgg tggtataata gttactatgc
     7261 catttgttaa tgaaaagaga aaaatgggtc ttttagtctg tgcatgtgga cctaatgaag
     7321 agagaatgag agatatagca actaaaatgg gagttactga tatagtatca gttcaaaaat
     7381 gtaaacaagc tcaagaaatt aaaggtgcat taaaatgtga aaatccagga cactgtccag
     7441 gacaagctca aaaatgtata gaatttaaga aagctggagc agaagttata ttaataggaa
     7501 actgtactga ctgtagtaac acagtaatgg gttctgcacc aaaattaaaa ttaggtgtat
     7561 atcatattac tgaccatgta atgagaacag ttaatcaccc attaataaga agaataaaag
     7621 gtgacgtaag attaaaaata aaataataga taaatagaag ttagacatcc ttagaagtaa
     7681 aacttaaaag ttacttctaa ggatgttttt taatgtattt tgagggcaat atatatattg
     7741 taaaataaat ggaaaaaata gacaaaatat gctagatatt gtataatgat aacaatagct
     7801 attacaatta atattggaca aaaataatga tactcaaata tgtggtttgt attaatatgc
     7861 atattatatt aagtcaacta tccaatagag taaaaggttt ttgtgatgtt tgaagatact
     7921 ctatagtatc ttttttatta taaaaactaa tttttgtctt ttattagata agtatctagg
     7981 atactaattt atattttatt aagttgttaa aaagtgcact taaaaagtta aaaaagaaaa
     8041 attaaaaaac ctatttatag aaactgtgaa gtatatctta aacttgggca cttaaaagat
     8101 atatggagtt agtagtgcaa cctgctataa atataaatag gagtaacttt taattgtcaa
     8161 aaatatataa aataagtaca taaataagta attaatagaa gatagatata aatatatttt
     8221 tgaaatgctc cctgcattca taacgaatgt agagtggcag atatctgccc aaaactcctt
     8281 taattgctgg aagttcctaa agctaactaa accacaacat aatcatgaaa taaggataag
     8341 tgtgatagag tgtgcattta ataagcactt gaggttacga aagtagaaaa aattagttag
     8401 atagcataag gttaaatcct aagtgccttg aaaatggata atcagcaggt aagcttcgaa
     8461 aagaagaaac ttcaacgact agagagaaat ctcgtaccta caagtgtggg gaaatgggga
     8521 gtacctaatt tatggttagg taatgatata gtctgtgctt aatagaaata ttaagaagtt
     8581 cataggagaa ctggttagga gtaacgaccc taattgaaca cactaaagat atattattga
     8641 ataagattca tataatattt aataatttta attatattaa gtgaatacaa tatatgtttt
     8701 tagtatcagg agaattttga aaacaaaaat taaaaaatca agtataatca gtttagtaat
     8761 tgcttttagt atggtattta ctacatttac accaatagta tcttatgctg acgaagtgac
     8821 tagcaatgat acaatactta atggagaaga acaatcaaat ggtcagacac cagatgtaga
     8881 gaaaccatca gatggtcagg taccagatgg ggaaaaacca tcagacggtc agataccaga
     8941 tggggaaaaa ccatcagacg gtcagatacc agatggggag aaaccatcag acggtcagat
     9001 accagatggg gaaaaaccat cagacggtca gataccagat ggggagaaac catcagatgg
     9061 tcagatgcca gatggggaga aaccatcaga tggtcagata ccagatgaga agaaaccact
     9121 agatgagcaa actccagaag aagaaaaacc attggaagaa gaaatagtta tagaggaaat
     9181 gagtttaaaa caagatattg ataaaattct tgatatgact atatctcaaa tcaataaaat
     9241 agtatataac ttttgggaag atgaagaaaa tgtaaaggca gatgaacaat cagaaattaa
     9301 ccaagtcttt acttcagaag actcgtttat tagcttatgg tatgacaaaa aagcaaaggt
     9361 aaaaaattct tgtttactaa aagaaataga tggttcagat agaccttatc atgatttaag
     9421 atttgatgat attacaaaga ctttaacttt tgactatgta ataaatggtc ttgtaggagc
     9481 aagctcaaaa gacaacaaat atgtaataga aggtgaagat ggagagcaaa ctgcattttg
     9541 ttacaataat catttaagac caccaagtag tgatggaaag agtccatatt taccagcaga
     9601 agaatttagt ggtcaagaaa atcaaaatga agatgcagtt aaatctatac tatatgcagg
     9661 ttctgaattt gatggatttg gatataagca acaatttaat ttatgcgaag aagaaaatga
     9721 cataatgact tactcagcta ctcaaagtgc aatttggatt attcttggac aaatagatga
     9781 acaagaaaga cttgaccagt atcaaggttc tataaacctt tgtgataaac ttatagaaag
     9841 agcaaaaact gaagaggaaa gagctgagta tcagaagaag aaagaagtag cgataaatac
     9901 aaaagcttat ttagaagcat tgttaaaagc aggtcgtgaa gagttaaagc cgaatgatac
     9961 aggaaaacct agtttgagta atggtttaac taagattaac tttgaaaaaa atgaagatgg
    10021 tacttatgaa acagaagctg tagccttagt aggttatagt ggtgtagtaa agttaatact
    10081 accagatgga gtaactgctt atgatggaga tggtaatata ataggtacag gtgaagtaga
    10141 aatttcaaca caacagaaat ttaaacttaa gagtgttgga aaaccagatg ccaaagctaa
    10201 tatttctgct gtatcttatg attatatttt tccaaaagct attcaatatt ataaggcagt
    10261 gcttgattta ggacaaaaag accattcatc ttcattacca tctagtaagc aaaatttatt
    10321 aagttataca atagagaaaa agaatggtca agaagttaac tttaatatag gtctaccaac
    10381 agatgatgat aatgttgtaa atccaccagt gccaccaata gatgatgatg ttgtaaatcc
    10441 accagtgcca ccaacagatg atacaaatgg acataagcca aaaccatcac caccaataga
    10501 tgatacagta ataaatccac cagtaccacc aacggatgat acagtaataa atccaccagt
    10561 accaccaacg gatgatgcag tgctaaatcc accagtacca ccaacggatg atgcagtgct
    10621 aaatccacca gtgccaccaa cggatgatgc agtgctaaat ccaccagtac caccagtaag
    10681 tgatacagtg gaaaaaatgc cagaactatc aagagatgat acaatagtaa aatcaccaaa
    10741 aacaggtgat gaaactcagc taatgtcata tgtatttatt agtgtcattg caatttgtgg
    10801 attagtgtat caatgtaaaa taaaaagaaa ttaatctaga ttattattaa gtttaaaata
    10861 aaaagtctca aattgattta taagtcattt tgggactttt ttatgtaaat ataatatgca
    10921 aatatagttt gtttaaaaat atctatttta attatattct taaatttatg attagataca
    10981 ggtattcatt aattgtttga tgaatagaaa aatgttttga aggtgaaaat agtattgaaa
    11041 aaat
//
LOCUS       DAEPXF010000128        10904 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain HB27
            SAMN13376389-rid12479423.denovo.135, whole genome shotgun sequence.
ACCESSION   DAEPXF010000128 DAEPXF010000000
VERSION     DAEPXF010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13376389
            Sequence Read Archive: SRR10518432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10904)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10904)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001069624.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001069624.1
            Genome Coverage       :: 315x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/15/2021 01:54:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,165
            CDSs (total)                      :: 4,076
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 89
            rRNAs                             :: 10, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 10 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 19 of 69
            Pseudo Genes (incomplete)         :: 44 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 10 of 69
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10904
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13376389-rid12479423.denovo.135"
                     /strain="HB27"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="China: Hebei"
                     /collection_date="2017"
     gene            135..701
                     /locus_tag="KRL67_002786"
     CDS             135..701
                     /locus_tag="KRL67_002786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBH1523652.1"
                     /translation="MLGEKMRNIRKSKKKTLSDVSKLTDLSISYISQIERDAIEPSLS
                     SLRKIAEVLDTPLYMFMDDNNTDDLVIRKEDRVMMKFPKSEMFYEIVSPMPTTEFTPS
                     ILFVEFELKPESEDAKDYIYHASEEIMVVTSGVVDICMGEKVVRLNPGDSTFIKSNVP
                     HKIINPSKDTIARGYGVISPPIWPIKSK"
     gene            complement(920..1906)
                     /locus_tag="KRL67_002787"
     CDS             complement(920..1906)
                     /locus_tag="KRL67_002787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin-dependent carboxyltransferase"
                     /protein_id="HBH1523653.1"
                     /translation="MGFKVELGGFQTLIQDRGRVGYGQYGVSGCGAMDEYAHRVGNIL
                     VGNSEDEASLEVLMLGPTITFDEYTQIAVTGGDLGAKINGKEIQNWRSYQINPGDVLS
                     FRGVKSGARAYVSIAGGIDVPLAMGSKSTYTRAKIGGFEGRALKKGDYINTFIQEKDF
                     TINKKLNSKYIPTYSSEIVLRIVKGPQFDAFSKGEVEKFLSNEYKVTNEIDRMGCRLD
                     GESIKHLNGADIISDGISYGAIQVPGHGKPIIMLSDRQTSGGYTKIGNVISVDLYKLA
                     QAKPNDVVKFELVDIYEAHRLLREQEDKIQDIYKSMKNIRVVKAKVLSDIAV"
     gene            complement(1936..2631)
                     /gene="pxpB"
                     /locus_tag="KRL67_002788"
     CDS             complement(1936..2631)
                     /gene="pxpB"
                     /locus_tag="KRL67_002788"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpB"
                     /protein_id="HBH1523654.1"
                     /translation="METRYLISGDKAVVAEFGNEISEDINKKVISFMRAVEISNLKGI
                     VTEMVPTYRSLMISYNPLEIDFDSLIENLKKIEDNLESIVLPKPKIHEIPVCYDEVFG
                     IDIKNVASYNNLTVDEVIKIHTSREYLIYMLGFTPGFPYLGGMDERIATPRLEVPRTK
                     IYGGSVGIAGSQTGVYPIDSPGGWQIIGRTPLKLYDENREEQILLRAGDFIKFVPITL
                     DEFIEIEKNNLSI"
     gene            complement(2651..3871)
                     /locus_tag="KRL67_002789"
     CDS             complement(2651..3871)
                     /locus_tag="KRL67_002789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent metal cation transporter"
                     /protein_id="HBH1523655.1"
                     /translation="MSTKNITDKTKNKKDVGALIGAAFIMATSAIGPGFLTQTAQFTQ
                     DFGPNFSFVILITTILFIGAQVNVWRVIGVSGLRGQDIANKIIPGLGYLVAFLVALGG
                     LAFNIGNVGGAALGMNVMFNMNMTLGTVLSGLIAIFVFMSKNSNSLVDKITKFLALGM
                     IIIVGYVAISNHPPVGEAVSRMVRPENPKGLIFPIITLLGGSVGGYITFAGGHRLIDG
                     GITGEENIKEITKSSLLGILVATMMRVLLFLAILAVVSKGLQLDPENPAASAFKFSAG
                     AIGYKFFGLVLWSAAITSVIGAAYTSVSFLKTLNPFIDKYEKYFIIAFIAISTLIMAF
                     IGKPATLLILAGALNGLILPITLGIMLIASKRKDIVGDYKHPTWLLIFGLIVVLISAY
                     TGITSLSSLGALFA"
     gene            complement(3900..4691)
                     /locus_tag="KRL67_002790"
     CDS             complement(3900..4691)
                     /locus_tag="KRL67_002790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LamB/YcsF family protein"
                     /protein_id="HBH1523656.1"
                     /translation="MYKVDLNSDLGESFGTYKIGLDEEVLKYISSANIACGFHAGDPS
                     HMEKTVQLAKKNGVKIGAHPGFLDLIGFGRREMKITKQEAKDYTKYQLGALMAFASSN
                     GCNIQHVKPHGALYNMAAKDKELAMGICEAIYEVDKDIILLGLYNSEMINSAKDIGLR
                     FANEVFADRAYDNNGFLVPRSVEGAVIHDTKHAIDRVVRMVKEGTVETLTGEVIHIKA
                     DSICVHGDNPKAIEFVKEIRKRFELESIEVCPLENIEVCSSENIV"
     gene            5279..5908
                     /locus_tag="KRL67_002791"
     CDS             5279..5908
                     /locus_tag="KRL67_002791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBH1523657.1"
                     /translation="MDKNYTMPIYQKIALDIANKIYTGEIQEDSVLFGRSVLAGKYNV
                     SPETIRRAVKILEDIGVVKSIKGKGVIVLSPDKASSFIKKYRDITNISSYKSTLYNLI
                     DTKSNLENEILDTINKILDYSNRLEIINPLVPVQFTINSNCKYIGQTAAKTKFWQNTG
                     ATIVAIKRGEELIISPGPYIEFLEGDILLVVGDQHIYNSIPMFLYENEK"
     gene            complement(6021..7535)
                     /gene="glpK"
                     /locus_tag="KRL67_002792"
     CDS             complement(6021..7535)
                     /gene="glpK"
                     /locus_tag="KRL67_002792"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HBH1523658.1"
                     /translation="MEKKYVMALDQGTTSSRAILFNKKGEIVKIAQKEFNQIYPKAGW
                     VEHDPMEIWGSQSGVMREVIETAGIRPEEIASIGITNQRETTVVWSRYTGKPIYNAIV
                     WQCRRTSEICDELKNKGLEESIKEKTGLLIDAYFSATKVKWILDNVEGAREKAEQGEL
                     LFGTIDTWLIWNLTRGKVHVTDYSNASRTMMYNINDLEWDEDILRELDIPISMLPLVK
                     PSSYVYGHTDERMLSGAKIPIAGCAGDQQAALFGQNCVEEGTAKNTYGTGCFLLMNTG
                     SNIVKSKHGLLTTIAWGVDGKVTYALEGSIFIGGASVQWLRDELKIIESAKDSEMYAN
                     RVEDTNGVYVVPAFTGLGAPYWDMYARGSILGLTRGAKKEHIVRATLESIAYQTKDVL
                     EAMQNDSKLKLKSLKVDGGASNNNFLMQFQSDILNVDIDRPKIVETTALGAAYLAGLS
                     VGFYIGRNEITSKWSVEKEFNPNMSEEKRCKLYKGWKKAVSRALSWEKEDELDI"
     gene            7778..8320
                     /locus_tag="KRL67_002793"
     CDS             7778..8320
                     /locus_tag="KRL67_002793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBH1523659.1"
                     /translation="MPKILENVKEDILKVSRDMILEEDYSNISIRKIAQRCGISAGTV
                     YNYFNSKQEIIEYITKSEWDLLIRRIEYSNKNTEDYIKKLNIIFTEIRNFINKVHNIQ
                     YNDFLNTFETERFFEMKKHKDDFHEQLSNKVYEALEKESFFNNDKLVCNIIIKIFFSY
                     STYLHIEFEDLKPYIEKLIH"
     gene            8334..10826
                     /locus_tag="KRL67_002794"
     CDS             8334..10826
                     /locus_tag="KRL67_002794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBH1523660.1"
                     /translation="MNHNTSIKNKDSNPKIFLVLTLYLLGIFMGAIDTGIVSPARTII
                     QNSLGVNEKTGIWMITIYTLTYASVIPISGKLADKIGRKYVYLVSIFLFGSGSLICGL
                     SSLFSNFYILLIGRVIQAIGGGGIMPIATAEFGTTFPENKRGMALGLVGATYGIANIL
                     GSSIGSTILSIFGTQNWKWLFFVNLPICLIILIGGVFCIKNNKSESKEKIDKLGTLML
                     ICIIVSLLYGLMNIDFFNFKNSIQDISVYPYLLTFIVLIPIFIFIENRAKDPILSFEY
                     FLNPRILIVLILSLIVGIGMMGMVFVPQYAENALKINAGSGGYFVAILGLFAGIAAPL
                     SGRLIDKFGAKKILLLGFSVSMIGSLYLVLIALKTNTVFSVCVSLIFMGLGMGFTMGT
                     PLNYMMLSNTRLEESNSALATLSLIRSIGTSISPAIMIGFIAHAGLSVQDNIMDLVGK
                     PSTPKIVQLEELENMIDDLKSDPEMAKNLKNVSIPNMNDTSNINMDMTGGKLPKSLLN
                     KVQSADTTNITNITKEISTTMFDEKVPSIITKIQKNVQKGIDGTQSGINGIEDGESKL
                     NSGIKGVQTGIDNMVKARAGINQGIDGVKKGIAGVDKGIKGMEQGLEKQDEAIKELTT
                     AYNKIPTTEKTPTDNENNSNEQNQSNTSNSESDDTKNDSTENKNNNSSENKPNVNEQK
                     ESLNAQIQQLKKTRDDLNTKLQKNKTQKKELSKKLRSMESQKKELQSKLNNSINKKKD
                     MEKSLDTMQQQKKSLQAVLAKTQEVKTEIPKAFDKSKLDYINSIENNRTKIENTLQST
                     LNLGFKQMYITVFCVNLLAFIILLFYKENKTR"
BASE COUNT         3807 a         1808 c         1364 g         3925 t
ORIGIN      
        1 ttttatttgc atttaattaa attttaattg tcaaaatcta ctatgtattc ttattgccac
       61 tacttctatg ttgtgctata attactttgt tttaagcgaa tattttatat taattttatt
      121 atttggaggt tcttatgcta ggtgaaaaga tgagaaatat aagaaaaagc aagaaaaaaa
      181 cattaagtga tgtttccaaa ttgacagatt tatctataag ttatatctct caaatagaaa
      241 gagatgctat tgaaccatcc ttgtcttcat tgagaaaaat cgctgaagtt ttagacactc
      301 ctttatatat gtttatggat gacaacaata ctgatgattt agtaattaga aaagaagata
      361 gagttatgat gaaatttcca aagagtgaaa tgttttacga gatagtatct ccaatgccta
      421 caactgaatt tacaccttca attttatttg ttgagtttga acttaaacca gaaagtgagg
      481 atgctaaaga ttatatatat catgcatcag aagaaattat ggtggtaacg agtggtgtgg
      541 ttgatatttg tatgggcgaa aaggtagtta gattaaatcc aggtgactca acatttataa
      601 aatctaatgt accacataaa atcatcaatc caagtaaaga tacaatagct cgtggatatg
      661 gagttatatc acctccaatt tggccaataa aatctaaata aactcaatat aacataccta
      721 ttttttgtag ataatacaat aaatttaaat atcactttta ttttaaaatt gaagtattta
      781 tttaaattta aaatatttac tcaaactaaa aaaatagcta tttgactaat ctaaaccatc
      841 ttaattatgg taaatagtaa atagctattt gtaagttata ttataaatta taataaaaat
      901 tgtatttgtt aaaattaaat taaactgcta tatcacttaa tacttttgcc tttacaactc
      961 ttatattttt catactcttg tagatatctt gaatcttatc ttcttgctct ctcaatagtc
     1021 tatgtgcttc atatatatct acaagttcaa attttactac gtcatttggc tttgcttgtg
     1081 ctaacttata caaatctaca cttataacgt tccctatctt agtatagcca ccactagtct
     1141 gtctatctga taacattata ataggttttc catgaccagg tacttgtata gctccataag
     1201 aaataccatc agatataata tctgctccat taaggtgctt tatactctct ccatctagtc
     1261 tacaacccat tctatctatt tcattcgtaa ctttgtattc atttgataaa aatttctcta
     1321 cttcaccttt agaaaaagca tcaaattgag gaccttttac aattcttagt acgatttctg
     1381 aactataagt tggaatatat tttgaattta attttttatt tattgtaaaa tctttttcct
     1441 gaataaaagt atttatataa tctccttttt taagtgctct accttcaaac ccaccaattt
     1501 tagctcttgt ataagttgac ttacttccca ttgcaagtgg aacatctatt ccaccagcta
     1561 ttgacacata ggctctagca cctgatttca cacctctaaa tgataataca tcacctggat
     1621 ttatttgata tgacctccag ttttgaattt cctttccatt tattttagcc cctaaatctc
     1681 ccccagttac tgctatttgc gtatattcat caaaggtaat tgtagggcct aacattaaaa
     1741 cttctaaaga agcctcatct tcagaatttc ctactaatat atttcctact ctatgtgcat
     1801 actcatccat tgctccacat ccagatacac catattgacc atatccaact ctacctctgt
     1861 cttgtattaa tgtttggaaa cctcctaatt caactttgaa tcccatatat accactccct
     1921 gtactataac gatatttaaa tacttaagtt atttttttcg atttctataa attcatctaa
     1981 tgttattgga acaaatttta taaagtctcc agctctaagc aaaatctgtt cttctctatt
     2041 ttcatcatat aattttaaag gtgtcctacc aattatttgc catccaccag gactatcaat
     2101 aggatataca ccagtctgag aacctgctat tccaacgctt ccaccataaa ttttggtcct
     2161 tggcacttct aatctaggtg tagctattct ttcatccata ccacctagat atggaaatcc
     2221 aggagtaaac cctagcatat atatcaagta ctctctactt gtgtgtattt ttataacttc
     2281 atctacagtg agattattgt agcttgcaac atttttaata tctataccaa aaacctcatc
     2341 atagcatact ggaatctcat gtattttagg ttttggtaat actatacttt ccaaattatc
     2401 ttctattttt tttaaattct caatcaaact atcaaaatct atttctaatg gattataact
     2461 tatcataagt gacctatatg ttggcaccat ttctgttaca atacctttaa gattcgatat
     2521 ttctactgct ctcataaatg aaattacttt tttgtttata tcttctgata tctcatttcc
     2581 aaattcagca accactgcct tatcccctga tatcaagtat ctagtttcca taaatgtcac
     2641 ctcttctaaa ttaagcaaat aaagcaccta ggctgctaag agaagttatt cctgtatatg
     2701 cagatattaa aactactatt aatccaaata ttaaaagcca tgtaggatgc ttgtaatctc
     2761 caacaatatc ttttctttta gatgctataa gcataattcc taatgtgatt ggaagtatca
     2821 atccatttaa agcccctgct aaaattaata aagtagctgg ttttcctata aatgccatta
     2881 taagtgttga tattgctata aatgctatta taaaatattt ttcatattta tctataaaag
     2941 gatttagtgt ttttaagaaa gaaactgatg tataagcagc tcctataact gatgtaattg
     3001 cagcagacca taatacaagt ccaaagaatt tatatcctat tgctcctgca ctaaatttaa
     3061 aagctgaagc agctggattt tctgggtcaa gttgaagacc ttttgaaact actgctagta
     3121 ttgctaaaaa taaaagaact ctcatcattg tggcaactaa tatacccaaa agagaagatt
     3181 tagttatttc ttttatattt tcttctcctg ttattccacc atctattaat ctatgtcctc
     3241 cagcaaaagt tatataacct ccaacacttc ctccaagtag agttattatt gggaatatca
     3301 atccttttgg attttctggt cttaccattc ttgatactgc ctctccaaca ggaggatgat
     3361 ttgatattgc tacatatcca actataatta tcattcctaa tgctaaaaat ttagttatct
     3421 tatccactag agaatttgaa ttttttgaca taaatacaaa tatggctatt aatccactta
     3481 atacagttcc taatgtcata ttcatattaa acattacatt cataccaagt gcagctccac
     3541 caacatttcc tatattaaaa gctaatcctc caagagctac taaaaaagct actaaatagc
     3601 ctaatccagg tattatttta tttgctatat cttgacctct aagtcctgat acaccaatta
     3661 ctctccatac atttacttga gcccctataa aaaggattgt tgtaattaaa attacaaatg
     3721 aaaaattcgg tccaaaatct tgggtaaatt gtgcagtctg agttaaaaac cctggtccaa
     3781 ttgctgatgt tgccattata aatgcagccc ctataagcgc tccaacatct tttttatttt
     3841 tagttttatc tgtaatattt tttgtactca tattttatct ccccctcatg ttatcagtct
     3901 tatactatgt tctctgatga acaaacttct atgttttcta atggacaaac ttctatactt
     3961 tctaattcaa aacgctttct tatttctttg acaaattcta ttgcttttgg attgtcccca
     4021 tgtacacata tagaatctgc ttttatatgt ataacttcac ctgttaaagt ttctacagtt
     4081 ccttctttta ccatcctaac aaccctatct attgcatgtt ttgtatcatg tataactgct
     4141 ccttctacac ttcttggaac caaaaaacca ttattatcat aagctctatc tgcaaatact
     4201 tcatttgcaa acctaagtcc aatgtcttta gcagaattta tcatttcact attatatagc
     4261 cccaacagta taatatcttt atcgacttca tatatagctt cacaaatccc catagctaac
     4321 tctttatctt tagctgccat attgtataat gctccatgcg gttttacatg ttgtatatta
     4381 cagccatttg aacttgcaaa tgccattaaa gcacctaatt gatattttgt ataatcttta
     4441 gcttcctgct tcgttatttt catctcacgt cttccaaatc ctattaaatc taggaaacct
     4501 ggatgtgcgc ctattttcac tccatttttt ttagctagct gtactgtttt ttccatatga
     4561 ctagggtcac ctgcatgaaa cccacaagct atattagctg atgaaatata ttttaaaact
     4621 tcttcatcta aaccaatctt atacgttcca aaactttctc ctaaatcact atttaaatct
     4681 actttataca aattatgacc cctccttgct aacattttga tagaataagt agttttaatt
     4741 tttaaatttt gtttatttta tatattgcat gaaatatttt atcacttgta ttcaatttta
     4801 tttttgcaac tattctattg catttaatta atataatttt accatttaag gaatattttt
     4861 gcaattattt taattaaaca ttcatttgta tttctttttc aaaaaatgtt tttaccattt
     4921 tttagttaat aattaattat tttcacttaa aacaaggggt ttaaccttca tttttaaagt
     4981 atttttcatt tttaatcact ttatatgcaa gacagaatca tttttcttca ttcatttgtt
     5041 tctttcatct atataattat cattctttgt aagtaaaata tctttatatt tttataatta
     5101 attctttaca tagttttaag tataataaaa tcatgtatac taaatgcatg ttaaaatatt
     5161 attttaaaac atgcacaata tttgtacata ttgtagacat ataaacctat aaattataca
     5221 atagtattgt taagattata tattttttta acataaattt tgtattgggg gaaaaacaat
     5281 ggataaaaac tatacgatgc caatatacca aaaaatagcg ttggatattg caaataaaat
     5341 atacacgggt gaaatacaag aggattcagt attatttgga cgctctgttt tggctggtaa
     5401 atacaatgta tctccagaaa cgataagaag agctgtcaaa attttagagg acattggcgt
     5461 tgttaaaagc ataaagggta aaggtgttat tgtactatct ccagacaaag cttcttcttt
     5521 tataaaaaaa tatagagata ttacaaatat atcatcatat aaatcaacac tttataattt
     5581 aatagatact aaatctaatt tagaaaatga aatattagat actataaata aaattttaga
     5641 ctattctaac agacttgaaa taatcaatcc tttagtgccc gttcagttca ccataaattc
     5701 taattgcaaa tatattggtc aaacagctgc aaaaactaaa ttttggcaaa atacaggcgc
     5761 tacaatagtt gcaattaaaa gaggtgaaga acttataata tctcctggac catatattga
     5821 atttttagaa ggagacattc tcttagttgt tggtgaccaa cacatttaca attctatacc
     5881 aatgttttta tatgaaaacg aaaaataaat tatataattt agtatgatta tataaaaatg
     5941 caaaattaac aacaaaaaag agcttcatat tttacataaa gctcttttta aattcaaata
     6001 aatttaactt tgattaattt ttaaatatcc aattcatctt ctttttccca gcttaaagcc
     6061 ctcgatacag cttttttcca gcctttatat aatttacatc tcttctcttc actcatattt
     6121 ggattaaact ctttttctac tgaccacttt gaagtaattt catttcttcc aatataaaat
     6181 ccaacagaca accctgctaa ataagcagct cctaaagcgg tagtttctac tattttgggt
     6241 ctatctatat ctacatttaa tatatctgac tgaaattgca ttaaaaaatt attattactt
     6301 gctccaccat caacttttaa tgattttagt tttaattttg aatcgttttg cattgcttct
     6361 aaaacatcct ttgtctgata agcaattgat tccaatgtgg ctcttactat atgttctttt
     6421 tttgctcctc ttgtaagtcc taaaattgaa cccctagcat acatatccca atacggagct
     6481 ccaagacctg taaatgctgg cactacatat acaccattag tatcctctac cctatttgca
     6541 tacatttcac tatcttttgc agattcaatt atctttaatt catctctaag ccattgaaca
     6601 gatgctcctc caataaatat acttccttct aatgcatatg ttacttttcc atcaactccc
     6661 caagcaattg ttgtaagtaa cccatgctta gatttaacta tgttactgcc tgtattcata
     6721 agtaagaaac aacctgttcc ataagtattt tttgcagtac cttcttcaac acaattttga
     6781 ccaaataatg ctgcttgttg gtctccagca catccagcaa taggtatttt agctcctgat
     6841 aacatgcgct catctgtatg accataaaca tagctagaag gttttactaa tggcaacatg
     6901 cttattggta tgtccaattc tcgtaatata tcctcatccc attctaaatc atttatattg
     6961 tacatcattg ttctagatgc atttgagtaa tctgttacat gtacttttcc acgagttaaa
     7021 ttccatatta accatgtatc tatagttcca aataataact ctccttgttc tgctttttcc
     7081 ctagctcctt ctacattatc taatatccat tttacttttg ttgctgaaaa gtatgcatct
     7141 attaaaagac ctgttttttc ttttattgat tcttccaatc ctttattttt aagttcatca
     7201 catatttctg atgtcctcct acactgccat actattgcat tataaatagg tttcccagta
     7261 tacctactcc aaacaaccgt agtctctctt tgattagtta ttcctattga agcaatttct
     7321 tctggtctta tacctgctgt ttctataact tctctcatta ccccactttg tgacccccat
     7381 atttccatag ggtcatgctc tacccatcca gcttttggat aaatttgatt gaactctttt
     7441 tgtgcaattt tcactatttc gcctttttta ttaaatagta tagctcttga actggttgtt
     7501 ccttggtcta atgccattac atacttcttt tccatcacca tattctcctt atcatataat
     7561 atttaattta ttaaaaagaa aaatttaata ttacatattt caataaataa tcatacttaa
     7621 gtacaaattt aatataatag atatttataa gaagacactt attttctatc atgtctatat
     7681 aataatatta tacattatag cttacatatt gattaataaa ttttaaagga gtataataaa
     7741 cagtgttcat aataatttta ataaaagagg tggtaatatg ccaaagattt tagaaaatgt
     7801 aaaagaagat atattaaagg tatctcggga tatgatttta gaagaagatt actcaaatat
     7861 tagcataagg aaaatagctc aaaggtgtgg tataagtgca ggtacagtat ataattattt
     7921 taattcaaaa caagaaataa tagaatatat cacaaaatct gaatgggatt tattgataag
     7981 gcgaattgaa tactccaata aaaatactga agattacata aaaaaactaa acattatttt
     8041 tactgaaata agaaatttta taaacaaagt tcataatatc caatacaatg actttttaaa
     8101 tacttttgaa acagagagat tttttgaaat gaaaaaacac aaagacgact ttcatgaaca
     8161 gctttcaaat aaagtatatg aagctttaga aaaagaaagt ttttttaata atgacaaatt
     8221 agtatgcaat atcataataa aaatattttt ttcatattca acatatcttc atatagaatt
     8281 tgaagattta aaaccctata ttgaaaaatt aatacattaa ggtggtgttt accttgaatc
     8341 ataatactag tattaagaac aaggacagta atccaaaaat atttttagta cttacacttt
     8401 atctattagg tatatttatg ggagctattg atactggaat agtatcacca gcaagaacga
     8461 ttatacaaaa tagccttgga gtaaatgaaa aaacgggaat ttggatgatt accatataca
     8521 cattaacata cgcttctgta attcctatct ctggaaaatt agcagataaa ataggaagaa
     8581 aatatgtgta tctagttagt atatttctat ttggtagtgg ttcattaata tgtggattat
     8641 catcactatt ttccaatttc tatattttat taattggtcg tgttattcaa gcaattggtg
     8701 gtggtggaat catgccaatt gctacagcag aatttggaac taccttccca gaaaataaaa
     8761 gaggcatggc tttaggttta gttggggcaa catatggtat tgccaatatt ttaggttcaa
     8821 gcattggtag cacaattcta agtatatttg gaactcaaaa ttggaagtgg ctattttttg
     8881 taaaccttcc aatttgttta atcatactta ttggcggagt tttctgtatt aaaaacaata
     8941 agtctgaatc taaagaaaaa atagataagc ttggtacatt aatgttgata tgtataatcg
     9001 tatcactatt atatggtctt atgaatatag acttttttaa ttttaaaaat tctatacaag
     9061 atatatcagt ttatccatac ttattgacat ttattgtact tatacctatt ttcatattta
     9121 ttgaaaatag agctaaagac cctattttaa gctttgaata ttttttaaat ccgagaatat
     9181 tgatagtact tattttatct ttaattgttg gtattggaat gatgggtatg gtattcgttc
     9241 cacagtatgc tgaaaatgca ttaaagataa atgctggttc tggtggatat tttgtagcta
     9301 tattgggatt atttgcaggt attgctgctc ctttaagtgg aagacttatt gacaagtttg
     9361 gggcaaaaaa aatactatta ctaggatttt ctgtatctat gataggctct ctttacctag
     9421 ttttaattgc tctaaaaacc aatactgtat ttagtgtatg tgtaagttta atatttatgg
     9481 gattaggaat gggatttact atgggaactc ctctaaacta tatgatgtta tcaaatacaa
     9541 gattagaaga atctaattca gcacttgcaa cattatcctt gatacgctca attggtactt
     9601 caatttctcc agctataatg attggattta tagcgcatgc aggattatct gtgcaagata
     9661 atattatgga cttagttgga aagccctcaa ctcctaaaat agttcaactt gaagaacttg
     9721 aaaacatgat agatgactta aaatcagacc ctgaaatggc aaaaaattta aaaaatgtct
     9781 ctatcccaaa tatgaatgat acttcaaata taaatatgga catgacaggt ggcaaactcc
     9841 caaaatcctt acttaataaa gttcagtcag cagatacaac caacattacc aatataacaa
     9901 aagaaatatc aacaacaatg tttgatgaaa aagttccatc tataattact aaaatacaga
     9961 aaaacgtaca aaaaggaata gatggaacac aaagtggaat aaacggtatt gaagacggtg
    10021 aaagtaaact aaatagtggt attaaaggag ttcaaactgg tattgacaat atggtaaaag
    10081 ctagagctgg tattaatcaa ggaatagatg gtgtaaaaaa aggtattgct ggtgttgata
    10141 aaggtataaa aggtatggaa caaggtcttg aaaaacaaga tgaggccatc aaggaactta
    10201 caactgctta taataaaata cctactacag aaaaaactcc tactgataac gaaaataact
    10261 caaatgaaca aaaccagagt aataccagta attctgaatc agacgacact aagaatgatt
    10321 ctacagaaaa taagaataac aattccagcg agaataaacc aaatgtaaat gaacaaaaag
    10381 aaagtttaaa tgctcaaata cagcaactaa aaaagacaag agatgactta aatacaaagt
    10441 tacaaaaaaa taaaactcaa aagaaagaat tgagtaaaaa actaagatca atggaatctc
    10501 aaaagaaaga gctacaaagc aagttaaata attctataaa caaaaaaaaa gacatggaaa
    10561 aaagccttga tacaatgcaa cagcaaaaaa aatcactcca agcagtttta gcaaagacac
    10621 aagaggtaaa aactgaaata ccaaaggctt ttgataaatc aaaattggat tatataaatt
    10681 ccatagaaaa taatagaaca aaaatagaaa acacactaca atctactcta aacttaggat
    10741 ttaaacagat gtatataaca gtattctgtg taaatctact agcatttata atattattgt
    10801 tctataaaga aaataaaact agataaaaga gtgttatagt attatattgt aaatttatct
    10861 tattcttaaa tattttatcc taaagaataa attcataaaa tttt
//
LOCUS       DAEPXF010000129        10870 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain HB27
            SAMN13376389-rid12479423.denovo.136, whole genome shotgun sequence.
ACCESSION   DAEPXF010000129 DAEPXF010000000
VERSION     DAEPXF010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13376389
            Sequence Read Archive: SRR10518432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10870)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10870)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001069624.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001069624.1
            Genome Coverage       :: 315x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/15/2021 01:54:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,165
            CDSs (total)                      :: 4,076
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 89
            rRNAs                             :: 10, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 10 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 19 of 69
            Pseudo Genes (incomplete)         :: 44 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 10 of 69
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10870
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13376389-rid12479423.denovo.136"
                     /strain="HB27"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="China: Hebei"
                     /collection_date="2017"
     gene            complement(373..1095)
                     /locus_tag="KRL67_002795"
     CDS             complement(373..1095)
                     /locus_tag="KRL67_002795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004398740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HBH1523661.1"
                     /translation="MITIKNLSKSFGDLNVLKNIDLEIAKGEIMVIVGPSGSGKSTLL
                     RCMNLLEIPTGGEIIFEGKNLVDKKTNIDEVRQNIGMVFQNFNLFPHKTILDNITLAP
                     IKLKKMTKEEAEKKAEILLSRVGLLDKKDSYPSQLSGGQKQRIAIARALAMEPDMMLF
                     DEPTSALDPEMVNEVLDVIKELAKEGMTMAIVTHEMGFAKEVADRVIFIDGGSILEDN
                     TPEEVFGNPKHERTKAFLAKVL"
     gene            complement(1154..1831)
                     /locus_tag="KRL67_002796"
     CDS             complement(1154..1831)
                     /locus_tag="KRL67_002796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HBH1523662.1"
                     /translation="MSLDFSFLSRFGTSFLEGTGVTVSISLVALCFGFIIGIIICMAK
                     ISKSKVLRAISSIYIEVLRGTPLLVQIYIVWFGLPQLGIRFPMLFGIPSEFIASAFAL
                     SVNSGAYVAEILRSGIQSVDNGQMEASRSLGLNYWSTMRYIIIPQAIKNILPSLANEF
                     ITLVKESSIISVIGVVEIMRTADIVKNAAFRALEPLIVAAAIYFVITFTLSRLVGLLE
                     KKLSVSN"
     gene            complement(1859..2674)
                     /locus_tag="KRL67_002797"
     CDS             complement(1859..2674)
                     /locus_tag="KRL67_002797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HBH1523663.1"
                     /translation="MKVFKKLLSLGLVLGLTLSLVGCSGGGEKTKLEQIKDNGKLVVG
                     TSAEFPPFEFHKVVDGKDSIKGFDIMLAEEFAKELGVKVEIKDMSFDGLIGALNADQV
                     DIVLAGMSPTPEREKSVDFSELYYLSRNAVIVKDADIDKVKTEDDLKKLRVGVQAGSI
                     QEEYVVNTLKMTTTKSLKAIPDLITELKNGNIDAVVTNEAVSLINVKKYDGIKMANTE
                     VGKDVTEGMAAAIKKSDNNKDFIELLNKKIKELQDGKKIEEFLNEASTEAASN"
     misc_binding    complement(2900..3110)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(3705..5771)
                     /locus_tag="KRL67_002798"
     CDS             complement(3705..5771)
                     /locus_tag="KRL67_002798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HBH1523664.1"
                     /translation="MVELIITTNIDIRKLNENQLEAVEHIDGPCMILAGPGSGKTRVI
                     TYRIANMVVNKNIAPTRILAISFTKASSIEMKNRALNLSDDIRLNKVTYGTFHSVFFK
                     ILRYFERYNLDSIFDEKSKRMTIKAILKSLNIENADDDENIGQVINEISYVKNELMDK
                     NEFNSEVLTKDEFLKVYNLYEEQKSKVNKIDFDDMLIRTYYLLLNNKSALEMVRNVYK
                     YILIDEFQDINKVQFEVLKLISSPLNNIFAVGDEDQSIYGFRGARPDFLLEFEQYFNN
                     TKKIILDINYRSKSEIVHTANRLIDKNKNRYEKVIKCSQGDGGSVTYISPHDSEEEAL
                     YIAREIIDEIQKDYVEYSDFAVIYRTNIQSRALVDVFMDMRIPFVVKDSVITIYDHWV
                     SQDILAYLRIGINPKSNKDWLRIINKPFRYISKDSINMVKDEKDFITALINKCNLHPK
                     QVKTINDLEIDLSYLNTLNPKNAISYIRTSLDYDRYVLDYCSNRKIKTNGLVEILNEL
                     ESSATNFNTVTEFLEHIDRVKTELTENNKNKQTEGVIFTTMHSAKGLEFRNVYIIGVN
                     EGTIPHEKSYDICKEEKKEEQLEEERRLMYVAITRAEEKLCISSTLNKYGKKVDKSLF
                     INDIKSPTKKEIDSIGVGDKVYHKRFHEGEIIKKDGIMFTIKFKDRERILDLKTCLLK
                     NIIYTI"
     gene            complement(5848..8877)
                     /locus_tag="KRL67_002799"
     CDS             complement(5848..8877)
                     /locus_tag="KRL67_002799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HBH1523665.1"
                     /translation="MENNLRKNNSIMKKMIFPLIIVMLIQTSLFCATILWGGTIKKLN
                     DNSFDILNERVINRKNYIQNEMLQRWSNTSETEATINSSVKKVLNEKNALIEDVGNNE
                     EINKDIIKSISNDLIYLLRKNSVTGAFIILSDKNINDKPIDSKGEISRTGLYIRDLDP
                     KFNPNDNSDLLIERGSSDIARSFGISMDSYWEPKFSFKSENEQEGDDFFYKPFRAAID
                     NPNVSSSNLGYWGSKFLLTNAGDRLMTYTVPLIYEDGTVYGVLGIDLSVNYLRELLPY
                     SEINGNKKGNYILGVDRNNDMAFENVISNGYSLKEKFLDGLHSNLEQKESYKNIYKIK
                     NKDNECGDGNIYASVQYIDLYNSNTPFENERWALLGIIEEKSLLSFSKRVEKLVIMSI
                     AISLLIGLIGIYIASRLFTKPIVLLANKVRNSNPSKPVNLDKINISEIDELSSAIELL
                     SANVADSSSKLSQIIEMVDMPIGAFELYKNAENVFCTKEFFSVFTTESKFEEYGYMEK
                     NKFLKKLRGLDKYLQENYETENTYIYKFESKDKKLKWVRMNIFEDELKILGVVVDVTK
                     EFIEKKKIEYERDFDILTNLLNRRAFHSQIQEKFKNKDELKIAAFIMWDLDNLKYIND
                     TYGHDAGDEYIRCAADILKKFISWNSIVSRLSGDEFYVFIYGYESKDSIRDIINSVKK
                     QMNNTLLKLHDGTEFKIRASAGISWYPDDSTNYDELMRYADFAMYQIKNTIKGEVSEF
                     DINIYNKDSFLLSNREELNKLIDGQLVEYAFQPIVDCKGKVFAYEALMRPKIKTFSSP
                     IDVIRLARSQSKLYQIERITWFKALKSFKKYKEKFGDCKLFLNSIPNNNLSKVDFEEI
                     EDLYGEYLNRVVLEITENDKINEDFIEKKKAYIDHWNIEIALDDFGTGYNGDAVLLYI
                     MPDYVKIDMSIVRGIDKDENRQKILKNLISYSNERNIKVIAEGVEMKGEMETLINLGV
                     DYMQGYYISKPSFIPEDISDKIKKELKICHENRNDVV"
     gene            complement(8930..10390)
                     /locus_tag="KRL67_002800"
     CDS             complement(8930..10390)
                     /locus_tag="KRL67_002800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HBH1523666.1"
                     /translation="MNRCKFAKLIITSMIVLLLTGCSLKKEDNYKLDPENPTSIQIWH
                     YYNGQQKMAFDKLVKEFNDTVGAEKGIIVEAFSQGNVVDLSEKVLDSANKKVGSEDVP
                     NIFAAYSDTAYEMDKLGLLVDMDKYISKEELNEYIDEYVEEGRINSKDEFKLFPIAKS
                     TEILMLNKTDWDKFAKATNSNINDIKTIEGLVETSKKYYEWTDSLTKEPNDGKAFFGR
                     DALANYIIIGSKQLGVEIFSVKDGKVTLNIDEKVMKKLWDNFYVPYINGYFASYGKFR
                     SDDAKTGDILALVGSSSGAAYFPDRVMLNDNESYNIETSVFQAPRFKGEGKYAVQQGA
                     GMAVTKSTKEEEYASVEFLKWFTDKEPNIKFSIASGYLPVKKKTNDLDVIKEVMKKDN
                     LSLSDKVEGSIASSIEQINSCNLYTNKAFDGGTKARQILEYSMLDKAKEDLNKINELV
                     KNGTYKKDAVAQFNNDKNFETWFESFKAKLENAIKK"
     gene            complement(10482..10856)
                     /locus_tag="KRL67_002801"
     CDS             complement(10482..10856)
                     /locus_tag="KRL67_002801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH1523667.1"
                     /translation="MGNKNKNTKYKDKTRERYNKKVENLNKEKLNKANKQSKKVDKID
                     DIINKSSIVLCVLLVVFSLLYLFKLVQLNIIMILLVITVAIQQGLVSFGAFRNKNYVK
                     ALFSIFVIVCFLIAFKYVVNPV"
BASE COUNT         3440 a         1931 c         1031 g         4468 t
ORIGIN      
        1 ttactataaa agttaaattt ataagcttac ttgttataaa aataactttt ataatttatt
       61 tcaaaaaaaa tattgatttt tgtgtcgcat tttgttataa taaattaagg ttatccccct
      121 ggtaacccta gttagttaat atatctattc ccaataccct ttcataagga tatattttat
      181 atttattaaa aatcggatta gtaacggaaa ttaattcggt ggaaaaaaga gcttaccccg
      241 agctcttttt tttctgcaaa aaatcgtaaa ataaagttaa tattaatata actttaattt
      301 taaaactaat attaaccatc actaaaaaag gatgtattga atttctaata tcaataacat
      361 cctttttata tattatagca cttttgctaa aaatgctttt gttctttcat gttttggatt
      421 tccgaaaact tcttctggag tattatcttc aagtatactt cctccatcta taaaaataac
      481 tctatctgca acttccttag caaatcccat ctcatgagtc acaattgcca ttgtcatacc
      541 ttctttagca agttctttta taacatccaa aacttcgttt accatttctg ggtcaagagc
      601 agatgttggc tcatcaaaca acatcatatc tggttccata gcaagtgctc tagctattgc
      661 tattctttgc ttttgtcctc cagataattg tgatggatat gaatcttttt tatctaaaag
      721 accaactcta ctcaacaaaa tttctgcttt tttctctgct tcttccttag tcatcttttt
      781 taatttaatt ggtgctaatg ttatattatc taaaatagtc ttatgtggga ataaattaaa
      841 gttctgaaac accataccta tattttgtct aacctcatca atattagttt ttttatcaac
      901 caaatttttt ccttcaaata ttatttctcc acctgtaggt atttctagta aattcataca
      961 tctaagaagt gtacttttac cagaaccact tggacctact ataaccatta tttcgccttt
     1021 agcgatttct aagtctatat tttttaatac atttaaatct ccaaaagatt tacttaaatt
     1081 ttttattgta atcatattat cactttcctt tttactattc tatgttttcc ctaaatgcta
     1141 aacatgctct catctagtta gacacagata gtttcttttc taataatcca actaatcttg
     1201 atagagtaaa tgttataaca aagtaaattg ctgctgctac tattaatggc tctaatgctc
     1261 taaatgctgc attttttact atatcagcag tacgcattat ctcaacaact cctattacag
     1321 atattattga agattctttt acaagtgtaa taaactcatt tgctaatgaa ggtaatatat
     1381 tttttatagc ttgaggtatt attatatatc tcatagttga ccaataattt aatcctaatg
     1441 atctacttgc ttccatttgc ccattatcaa ctgattgtat tcctgatcta agtatctctg
     1501 ccacgtatgc accagagttt actgataatg caaatgcact agctataaat tcacttggta
     1561 taccaaacaa catagggaat cttattccta attgaggtaa accaaaccat actatatata
     1621 tctgtactaa taatggtgtc cctcttaaaa cctcaatata gattgatgat attgctctta
     1681 atactttgct ctttgatatt tttgccatac agataattat acctattata aatccaaagc
     1741 ataatgccac aagagaaatt gatactgtca cacctgttcc ttccaaaaaa gaagtcccaa
     1801 atctacttaa aaaactaaaa tctaaactca taatttcatc tcccacataa ctttcttact
     1861 aatttgaagc agcttcagta gatgcttcat ttaaaaattc ttctatcttc ttgccatctt
     1921 gtaattcttt tatctttttg tttaataatt ctataaaatc tttgttattg tcagattttt
     1981 tgattgctgc tgccatacct tctgttacat ctttcccaac ttcagtattt gccattttta
     2041 ttccatcata ttttttaaca tttattaaag aaactgcttc gttagtaaca actgcatcta
     2101 tgtttccatt ttttaattct gtaattaaat caggtattgc ttttaaagat tttgtagttg
     2161 tcatttttaa agtgttaact acatattctt cttggatact tcctgcttga actccaactc
     2221 taagtttctt taaatcatct tcagttttta ctttgtctat atctgcatct ttaactataa
     2281 ctgcatttct acttaaataa tataattctg agaaatcaac actcttttct ctctctggtg
     2341 ttggagacat acctgcaaga acaatatcaa cttggtctgc atttaaagca cctattaatc
     2401 catcaaatga catatcctta atttcaacct ttacaccaag ctcttttgca aattcttcag
     2461 ccaacattat atcgaatcct tttattgaat cttttccatc tactacctta tgaaattcaa
     2521 atggaggaaa ttctgcgcta gtaccaacaa ccaattttcc attgtctttt atttgctcta
     2581 actttgtttt ttctcctcca ccactacatc ctactagcga taatgtcaat cctaatacta
     2641 atcctaaact taataatttt ttaaatactt tcattttaac tacccctctc ttttaattat
     2701 aatttccctc aaattttaat acttaaatgt ttcgagttac aaaaaaagtc tctcagtctt
     2761 taatgactaa gagacgactt taccgcgtta ccactcttat tgataaatac ttatattact
     2821 ttcaaaattt gtgactgtta gcttttaagc tgtatggata tttttatttt taaatcaaaa
     2881 aagtccctta gctttacagc taagagacga attatatccg cgttaccact ctttttgata
     2941 agtaagtact tatcctctca atcccttaag gcgggtaaac caattatcat actaaagttt
     3001 ctgataatct cctccagagc tctcttcaca tagacttcat tgctaggctt acaccaatcc
     3061 ctaactcgct gtaaatccat atatgctact ttctctatca ttggatttgt ttccttaaat
     3121 tgttaataca attgtacttc cttataatat ttttgtcaat aagtttttta tttttttaat
     3181 aatttttact tgtaaattat ctaaatacta ctgtttatat ttattgatat tatttgtgta
     3241 attataaata gtatatttca tatttttagc tatttcttaa ataatactaa ataatatttt
     3301 ctacatgctt gctttttatt taataatact aaataatatt ttctacatgc ttgcttttta
     3361 tttaataata ctagataata tctttcacat ggttgctttt tctttaataa tactaaatag
     3421 tatctttcac actgttgctt tttatttaat aatactaaat agtatctttc acactgatgc
     3481 cttttcttta ataatactaa ataatatctt tcacattgtt gctttttctt tagtaatact
     3541 aaataatatc tttcacattg ttgctttttc taaacaacta taaataatat attaaacctt
     3601 tatttctctt ttctaataag ttaaataata cattttacac atatctattt gtcttgcaca
     3661 taataaataa tacattatat ttctatctaa atttagattc taaattaaat agtataaatt
     3721 atattcttca ataaacaagt tttcaaatcc aagattcgct ctctatcttt aaactttatt
     3781 gtaaacataa ttccatcttt tttaattatt tctccttcat gaaatctttt atggtatacc
     3841 ttatccccta caccaatact gtcaatttct tttttcgttg gagattttat atcattaata
     3901 aataatgatt tatcaacttt cttaccatac ttatttaagg tagagcttat acataacttt
     3961 tcttcagctc ttgtaatagc aacatacata agccttcttt cttcttctaa ttgttcttct
     4021 tttttctctt ccttacaaat atcatatgat ttttcatgag gtattgttcc ttcatttaca
     4081 cctataatat aaacatttct aaattctaat ccttttgcac tatgcatagt tgtaaatata
     4141 acaccctcag tctgcttatt cttgttattt tccgttaact cagtctttac cctatcaata
     4201 tgctctaaaa actctgtaac agtattaaaa ttagttgctg aactctcaag ttcatttaat
     4261 atctctacaa gtccattagt ctttatcttt ctgttgctac aataatctaa aacgtatcta
     4321 tcataatcaa gactagttct aatataagat attgcatttt taggatttaa agtatttaaa
     4381 taacttaaat ctatttccaa atcatttatt gttttaactt gtttaggatg aagattacac
     4441 ttatttatta atgctgttat aaagtctttt tcatctttaa ccatatttat actgtctttg
     4501 gatatatatc taaaaggctt gtttataatt ctcaaccaat ctttatttga ttttggatta
     4561 atacctattc tcaaataagc tagtatatct tgacttaccc aatggtcata tattgtaatt
     4621 actgagtctt ttactacaaa tggaatcctc atatccataa atacatctac taatgctctt
     4681 gactgtatat tagttctata aattactgca aaatctgaat actctacata atctttttgt
     4741 atttcgtcaa ttatttccct ggcaatatat aaggcttctt cctctgaatc atgtggagaa
     4801 atataggtaa cacttcctcc atccccctga ctacacttaa tgactttttc atatctattt
     4861 ttatttttgt cgataagtct atttgcagta tgcactatct cacttttaga cctgtaattt
     4921 atatctaata ttattttttt agtattatta aagtattgtt caaattcaag caaaaaatct
     4981 ggtctagcac ctctaaatcc atatatactt tggtcttcat ctccaactgc aaatatattg
     5041 tttaatggac tagaaattaa ctttaaaact tcaaactgta ctttgtttat atcttgaaat
     5101 tcatctatca atatatattt atacacattt ctaaccattt ctagtgcaga cttattgttc
     5161 aaaagtaaat aataagttct tataagcata tcatcaaaat ctattttgtt aactttagat
     5221 ttttgttctt cgtataagtt gtatactttt aaaaactcat cttttgttaa tacttctgaa
     5281 ttaaattcat ttttatccat aagttcattt tttacatagg aaatttcatt tataacttgt
     5341 cctatattct catcatcatc agcattctct atatttaaac tttttaagat tgccttgatt
     5401 gtcattcgct ttgacttttc atcaaatata ctgtctaaat tgtatctttc aaagtatctt
     5461 agaattttaa aaaatacaga gtggaaagta ccataggtta ccttattaag tcttatatca
     5521 tcacttaaat tcaaagctct atttttcatt tctattgaag atgccttggt aaaacttatt
     5581 gccaagattc ttgtcggtgc tatgtttttg tttacaacca tattggctat tctatatgta
     5641 attactctgg tcttacctga accaggccct gcaagtatca tgcaaggccc atctatatgc
     5701 tcaactgctt ctaattgatt ttcatttaac tttcttatat ctatatttgt tgtgattatt
     5761 agctccacca ccctaattta taattatagt attttctttt taattataaa ctaaaagttc
     5821 ataaaaataa atttaatttt tcctctatta aaccacatca ttcctatttt catgacatat
     5881 cttgagctct tttttaattt tgtcacttat atcttcaggt ataaatgatg gtttactaat
     5941 atagtaacct tgcatataat ctactccaag attaattagt gtttccattt cacctttcat
     6001 ttctacacct tctgctatca ctttaatatt tctttcatta gaatatgaga tgagattttt
     6061 taatattttc tgtctgtttt catctttatc aatacctctt acaatagaca tatcaatctt
     6121 tacatagtct ggcattatat ataatagtac agcatcccca ttgtaacctg tcccaaagtc
     6181 atctaaagca atttctatat tccaatggtc tatatatgct ttctttttct caataaaatc
     6241 ttcattaatc ttatcattct ctgttatttc taatactact ctatttaaat attcaccata
     6301 taaatcttca atttcctcaa aatctacttt tgataaatta ttattaggaa tagaatttaa
     6361 gaacagtttg caatcgccaa atttttcttt gtatttttta aatgatttaa gtgctttaaa
     6421 ccatgtaata cgctcaattt gatataattt agactgtgaa cgagctaatc taatgacatc
     6481 tattggtgaa ctaaaagttt ttattttagg tctcataagt gcttcatatg caaatacttt
     6541 acctttacaa tctacaatag gctgaaatgc atactcaact agttggccat caattaactt
     6601 gtttaattct tctctattac tcaataagaa tgaatcctta ttatatatat taatatcaaa
     6661 ctcactaact tcacccttaa ttgtattttt aatttgatac atagcaaaat ctgcgtatct
     6721 cattaattca tcatagttgg ttgaatcatc agggtaccat gaaattcctg ctgatgccct
     6781 tattttaaat tctgttccat catgaagttt taataaggta ttattcatct gtttcttaac
     6841 agaattaata atatctctta tactatcttt actttcatat ccatatataa atacataaaa
     6901 ctcatcacct gaaagtctag atactatact attccatgat ataaacttct taagtatatc
     6961 tgcagcacac ctaatatatt catcaccagc atcatgtcca tatgtatcat taatatattt
     7021 tagattatct aaatcccaca taataaatgc tgctatctta agttcatctt tatttttaaa
     7081 tttttcttgt atctgtgaat gaaaagctct cctatttaaa agattcgtta aaatatcaaa
     7141 atctctttca tattctatct tctttttctc aataaattct tttgtgacat caacaacaac
     7201 accaagaatc tttaattcat cttcaaagat attcattctt acccacttaa gttttttatc
     7261 tttagattca aacttatata tatatgtatt ttctgtttca taattttctt gcaaatattt
     7321 atctagtccc cttaattttt taaggaactt atttttttcc atatagccat attcttcaaa
     7381 tttgctttca gtagtaaaca cgctaaaaaa ttcttttgta caaaaaacat tctctgcgtt
     7441 tttgtataac tcaaaagctc cgattggcat atctaccatt tcaataatct gtgataattt
     7501 tgatgaagaa tctgcaacat ttgcactgag taactctata gctgaagata attcatctat
     7561 ttcagatata tttatcttat caagatttac tggttttgat ggattactat ttctaacttt
     7621 gttggctaaa agaacaattg gttttgtaaa taatctcgat gcaatgtaaa tacctattag
     7681 acctattaat aaagaaattg ctatagacat tataactaat ttctctaccc tttttgaaaa
     7741 tgacaaaaga cttttttctt caataatacc caaaagagcc caacgttcat tttcaaatgg
     7801 agtattggag ttatataaat ctatgtattg tacactagca taaatattgc catcaccaca
     7861 ttcattatct ttatttttaa tcttatatat atttttatac gattccttct gctctaaatt
     7921 agaatgtaag ccatccaaaa atttttcttt taatgaatat ccatttgaaa ttacattttc
     7981 aaaagccata tcattatttc tatctacacc taaaatataa ttaccttttt tatttccatt
     8041 tatctctgag tatggaagta attctcttaa ataatttaca cttaaatcta ttccaagtac
     8101 tccatataca gttccatctt catagataag tggaactgta tatgtcataa gcctatcacc
     8161 tgcatttgtc aataaaaact tacttcccca gtatcctaaa ttagaacttg atacatttgg
     8221 attatcaatt gcagctctaa atggtttata aaaaaaatca tctccttctt gctcattttc
     8281 acttttaaat gaaaacttag gttcccaata actgtccata gatattccaa aacttcttgc
     8341 tatatctgat gaacctctct caattaacaa atcagaatta tcatttggat taaacttagg
     8401 gtctaaatcc cttatataca atccagtcct acttatttca cctttactat ctattggctt
     8461 atcgttaata tttttgtcac ttaggattat aaaagcccct gtaacagaat tttttcttaa
     8521 aagatatatt aaatcatttg atatactttt tataatatct ttgttaattt cttcattgtt
     8581 gcctacatcc tcaattaagg catttttttc atttaatact ttttttacag aggaattaat
     8641 tgtagcttcc gtttcacttg tatttgacca tctttgtaac atttcatttt gtatataatt
     8701 tttacgatta attacccttt catttaagat gtcaaacgaa ttatcattta atttcttaat
     8761 agttcctccc cataaaattg tggcacaaaa taaacttgtt tgaattagca taacaataat
     8821 taggggaaat atcatctttt tcattattga attgtttttt cttagattgt tttccatact
     8881 tattccacct atacctttgt gctcgtcgac acactaactg ccaattcatc tattttttta
     8941 tagcattttc aagcttagcc ttaaaacttt caaaccaagt ttcaaaattc ttatcattat
     9001 taaattgtgc tactgcatcc ttcttataag ttccattttt tactaattca tttattttat
     9061 ttaaatcttc tttagcttta tcaagcattg aatattcaag tatttgtcta gctttagttc
     9121 caccatcaaa tgctttatta gtatataaat tacaactgtt aatctgttca atagatgagg
     9181 ctattgaacc ttcaacttta tcagataaac tcaaattatc tttcttcata acttctttta
     9241 taacatccaa atcattcgtt tttttcttta ctggtaagta tccagaagca atagagaatt
     9301 ttatattagg ttctttatct gtaaaccatt ttaaaaattc tactgatgca tattcttctt
     9361 ctttagtgga ttttgttaca gccattcctg caccttgttg tacagcatat tttccttctc
     9421 ctttaaaacg tggtgcttga aaaacagatg tttcaatatt ataactctca ttatcattta
     9481 gcattactct atctggaaag tatgctgctc ctgaactaga acctacaaga gctaatatat
     9541 cacctgtttt tgcatcatca gaacgaaact taccataaga agcaaaatat ccatttatat
     9601 atggtacata gaaattatcc catagttttt tcataacttt ttcatcaata tttagtgtaa
     9661 ctttaccatc tttaacagaa aaaatttcta ctcctagttg tttacttcct ataataatat
     9721 aatttgctaa agcatctctt ccaaaaaatg ccttaccatc attaggctct ttcgttaaac
     9781 tatctgtcca ttcataatat ttttttgatg tctctactaa tccttcaatt gtttttatat
     9841 catttatatt tgaatttgta gcttttgcaa atttatccca atcagtttta tttaacatca
     9901 aaatttcagt tgattttgca attgggaaaa gtttaaactc atccttagaa tttattctgc
     9961 cttcttctac atattcgtct atgtattcat ttaattcttc tttacttatg tacttatcca
    10021 tatctactag taaccctaat ttatccattt catatgcagt atctgagtat gctgcaaata
    10081 tatttggtac atcttcagaa ccaacttttt tatttgcaga atctaaaact ttttctgata
    10141 aatcaactac attaccttga ctgaatgctt caacaataat tcctttttca gcacctacag
    10201 tgtcattaaa ttccttaaca agcttatcaa aagccatttt ttgctgtcca ttataataat
    10261 gccaaatttg tattgatgtc ggattttctg ggtctaactt ataattatct tccttcttta
    10321 aactacatcc tgtaagtaat aaaacaatca ttgatgtgat aattaatttt gcaaacttac
    10381 acctattcat aaaatctact ccttcaaatt ataaaattta taatagcatt aaaactgtaa
    10441 cttcactatt tatattaatt atagaacgtt tattgatata tttaaacagg atttacaaca
    10501 tatttaaaag caattaagaa acatactatt acaaatatag aaaataaagc ttttacataa
    10561 tttttatttc taaatgcacc aaatgacacc aatccttgtt gaatagctac tgtaattact
    10621 aaaagtatca ttattatatt aagttgaacc aatttaaata agtaaagtaa tgaaaacact
    10681 actagaagta cacataatac aatactagat ttattaataa tatcatcaat cttatctacc
    10741 ttcttagatt gcttattagc tttatttaat ttttctttat ttaaattctc aactttttta
    10801 ttataccttt ctcttgtttt atctttatat ttagtatttt tatttttatt tcccattcat
    10861 ttcccatcct
//
LOCUS       DAEPXF010000130        10843 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain HB27
            SAMN13376389-rid12479423.denovo.137, whole genome shotgun sequence.
ACCESSION   DAEPXF010000130 DAEPXF010000000
VERSION     DAEPXF010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13376389
            Sequence Read Archive: SRR10518432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10843)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10843)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001069624.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001069624.1
            Genome Coverage       :: 315x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/15/2021 01:54:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,165
            CDSs (total)                      :: 4,076
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 89
            rRNAs                             :: 10, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 10 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 19 of 69
            Pseudo Genes (incomplete)         :: 44 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 10 of 69
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10843
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13376389-rid12479423.denovo.137"
                     /strain="HB27"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="China: Hebei"
                     /collection_date="2017"
     gene            65..703
                     /locus_tag="KRL67_002802"
     CDS             65..703
                     /locus_tag="KRL67_002802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBH1523668.1"
                     /translation="MSELSKRDKEKIQRENEIIDKAEKLFCLNGFDSTTMSELAKEVE
                     YTKRTIYKYFSCKEDLFFAVVLRGYKRLWDNVKIESAKGKTGFEKIKLSYFAFHKFYS
                     VEPSLLCLMGMIGIVNSEKSDTDMLFKEKFFSFNKFMFDEIQEMFEIGKNDRSIRHDI
                     EIPTLMYSSIFTLTGFFNLLSVTGKSYLNNFNIDEERFIETTLALLIDSIKA"
     gene            848..1510
                     /locus_tag="KRL67_002803"
     CDS             848..1510
                     /locus_tag="KRL67_002803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBH1523669.1"
                     /translation="MAKILLLEDDDTITFGIKTSLEKKNHTVCCYNTITEAKKNFDKT
                     IDLILLDLNLPDGIGYEFCQYVKSIEDTPVIFLTVRDDEKDIIRGLDMGADDYVVKPF
                     LLSVLQSRINAVLRRTHSNTNNTITCGNISLNKVEVKAYLDNVEVPLTAGEYKLLLSL
                     MENKNRAMTRTKLLEMLWDVDGNFVNDNTLTVTIKRLREKLKNTPCIKTLRGIGYRME
                     DS"
     gene            1510..2691
                     /locus_tag="KRL67_002804"
     CDS             1510..2691
                     /locus_tag="KRL67_002804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBH1523670.1"
                     /translation="MKTPRNINSTVVFIIFCLIVTFLLSVILTTITSFNEYNVLSKAL
                     GASYEYNPKSTESILKSLKNSSDEDFKTGNEILSKHGYSKSTFWSKNFFYFLVVSFGI
                     ISTMTIVLYIIKHRTNKQKLSRINNLTQYLEEVNLGKESVLLRCEDEFSHLEDEIYKT
                     VGELRCSKEFALKERQTLSDNLADIAHQLKTPITSMSLMAQLLSENCPDEEIDYINRL
                     TNQISRLERLVSSLLTLSKLDTSTLIFESKLIDVHSLLTYAIEPIEGSLREKHQTFTI
                     EGSPSVEFEIDINWTLEALLNILKNCSEHIEDGGYIVAYYSQNPLYVEIIIEDNGNGF
                     AQEELPYIFNRFYRGKNASKDSIGIGLALSKSIIERQNGTLHAENCHNGGARFIIKFY
                     T"
     gene            complement(2872..5532)
                     /locus_tag="KRL67_002805"
     CDS             complement(2872..5532)
                     /locus_tag="KRL67_002805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HBH1523671.1"
                     /translation="MNILQEYTLDFVRRNKRSSIAIMLAILLTTTMMSSLCGLLYTMW
                     TDFIRLSIEKEGNWHGELFDDTYGKDLSLIENFSSVDDVMVKGQWYVGKINNDKRDYI
                     IYRNATSEYWNHMPEKDTIIEGRRPSNTNEIALSKQYFENNPSVKIGDKITLPIGDRI
                     LNGKPLEPIAPKSKGESFKKISETTLTIVGKVDVSTSSVIPAYTALGYLDKTSIKPND
                     SITVYLRFKNIRDTYKELPKLAKSLGWKTNEYGKYNLKYNSNYLLKMLVFSPEQKASM
                     SSISKFSTPIMYLTIAIFTVAVFVMVIYNAFSLSANARLTQLGILSSVGASPKQIKRS
                     VVFEGFLLTIIPLPIGLFLGWLLCNRLIVYINSVNYHTDAPEVVFTYGIPAFLPAVLL
                     TIVTVWISALIPARKVSKISPIEAIRQGDSVKIKKSHKYSLGKIFGIEGELASNALRA
                     RKKSYRTATISLTLSFLLLTGFLHIIANQEGAKAVYGSDNYKDQRDIAVYLENSEPVE
                     STFIEKLDNIEGVKSSLYYQKMSAATWLTEKDASKELAKNGGFKSIVSSKKYSPIERD
                     GKFRIISNIIGLDDKSFGEYCRQISVDPKLFYDTKNPTSIVYNKEEDVNRSTRRNKVY
                     IDYLSISIGDNIKLNEKVYDEDKGDYTYNMKVGAITSTLPKIVDTSSRYTLMQIVPMS
                     MVQEISKNYDEVSRLRSNRLIGLFKVNNRKDISRVREKLESICNKDYGSGDYSIEDVL
                     ESEKQDASGRKTMNLIVGFITGLLAMIGLSNVLATVSGNIRSRRQEFAMLRSVGLSPD
                     GIKKMLVLEGLFLGITPLLLSIPVQIGIVYTFLRINEIYFIEYLPFAPVSTIIGFTIL
                     ILFIVIASYMTGYKQLKNENIVESIKNETI"
     gene            complement(5529..6212)
                     /locus_tag="KRL67_002806"
     CDS             complement(5529..6212)
                     /locus_tag="KRL67_002806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBH1523672.1"
                     /translation="MEILKCENLTKIYGSNQTRVTALNNVNLSVQKGDFVSIVGASGS
                     GKSTLLHMLGGVDRPTSGKIYVEDTEISSLKEEALAVFRRRKVGLIYQFYNLIPTLDV
                     RKNILLPMLLDKRKVDEDRFSEIVSILGLSDRLNHLPSQLSGGQQQRVSIARSLIYRP
                     AILLADEPTGNLDRKNSEEIVDLLNLSNKRFNQTILLITHDEKIALEANRIVTMEDGV
                     IVSEKVVKK"
     gene            complement(6556..7494)
                     /gene="blaCDD"
                     /locus_tag="KRL67_002807"
     CDS             complement(6556..7494)
                     /gene="blaCDD"
                     /locus_tag="KRL67_002807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDD family class D beta-lactamase"
                     /protein_id="HBH1523673.1"
                     /translation="MKRKNNFIWIVILLVVVAMGIYYVGRKHNNTKQKYDKNYINSEL
                     NKSKIENNDKNKKEKINIVDYSDCFEGISGGAIFYNTKNKEYNIYNKELIETRRSPCS
                     TFKIVSTLIGLEKGVINSKESVMGYDGTNYPNKNWNKNLSLEEAFKESCVWYYKKLIN
                     KVDAKSVQNILDDLKYGNCDISEWEGDLKNGKGHLNGFWLESSLQISPKEQVQTMAKI
                     FEGDTNFKKEHINILKDIMKIDVNDKNINVYGKTGTGFDEKNKRVDAWFVGMLEREGD
                     TYYFAIKSDDSNKEITGPKVKEIAINIIKKYYSVRE"
     gene            complement(7522..8238)
                     /locus_tag="KRL67_002808"
     CDS             complement(7522..8238)
                     /locus_tag="KRL67_002808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH1523674.1"
                     /translation="MILIVILSIFIIASINIIILGKENIRDIVIPLTLFIVSDFLFLC
                     GNIICRGGVMLLFIGLFAIPLMLIAFIWICVHMIKLYYKKGLNKRKMIGCIFSIFISI
                     GTIFIPSPSESFRFKLYEKDYSVVAKAILKVYDEKKLSSECVFYIQSDKNELEKIFSK
                     EVIKKMKKLNRNLDVVKISGDRNLVYFFFGAELQSIDGIVIFKDLKHVKTKLDFINRS
                     IQAPRLEYITNNVYYFKGEL"
     gene            8570..10813
                     /locus_tag="KRL67_002809"
     CDS             8570..10813
                     /locus_tag="KRL67_002809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HBH1523675.1"
                     /translation="MSNIIIKGLTQNNLKNISFKIPKNKIVVFTGVSGSGKSSIVFDT
                     IAAESQRQMNETYSSFIRNRLPKYRKPQVELIDNLTASVIVDQTPLGGNARSTVGTIS
                     DLYSSLRLLFSRIGEPYIGTTSHFSFNDPNGMCKNCSGIGRVKVINIESIINPENSWN
                     TGCINDSLFKPNSWYWKQYAESGLFDLDKPIKDYSFEEYNLLLYGSKNKDGKQENPKI
                     DGIFNKYNQTYLNRDISNMSKHTKEKSERLITDKECPVCHGKRLNKETLNCNILGYSI
                     ADMCNMELTDLYDLLLQIKDKRTETVIKTLADGLKRMIDIGLPYLHLNRESSSLSGGE
                     AQRLKLVRYMGSSLTGMTYIFDEPSTGMHPRDVHRMNQLLKKLRDRGNTVLVVEHDKD
                     VISIADEVIDIGPSAGTQGGYVVFQGSYDKLLLANTLTSNAMNLSLPIKTNSRHPKGT
                     LPIRDACINNLKHIDVDLPVGIMTVVTGVAGSGKSTLISRVFAQKYEKDVIKIDQSPI
                     TATNRSMPATFLGFFDEIRKVFAKENNVEEGLFSFNSKGACPVCDGKGVLVTELVFMD
                     PIINTCEACKGTRYSEEVLSKLYKGKCIVDVLSMTVDEALEFFTEKKLCKILQTMKDV
                     GLPYITLGQPLSTLSGGERQRIKLAKNLNKKGTIYILDEPTTGLHGSDIEKLMLIFEH
                     IVDKGNTVIIIEHNLDVLKQADWVIDIGPDGGKNGGQVVFEGTPLEMFERGCTITSDF
                     LRKSIKI"
BASE COUNT         3822 a         1642 c         1459 g         3920 t
ORIGIN      
        1 aatattatat ttacataagt ataccatcag tatacttttt tatcaataga aaggaataat
       61 ttatatgtca gaattatcaa aaagagataa agaaaaaata caaagagaaa atgagattat
      121 tgataaggca gaaaaattat tttgtcttaa tggatttgat agtacaacta tgagtgagtt
      181 ggcaaaggaa gttgaatata ctaaacgtac catatacaag tacttttcat gtaaagaaga
      241 tttattcttt gctgttgtct taagaggata taaaagactt tgggataatg ttaaaataga
      301 aagtgcaaaa ggcaaaacag ggtttgaaaa aataaaactt tcatactttg cttttcataa
      361 attttactct gtagaacctt ctctattatg ccttatggga atgattggta ttgtaaactc
      421 tgaaaaatcg gatactgata tgctgtttaa agaaaagttt ttttctttta ataaatttat
      481 gtttgatgaa atacaagaaa tgtttgaaat tggaaaaaat gatagaagta ttaggcatga
      541 tattgaaata cctactctta tgtattcatc aatatttaca cttacaggat tttttaattt
      601 actatctgta acaggaaaat catacctgaa caactttaat atagatgagg aaagatttat
      661 tgaaacaact cttgctcttt taatagattc aattaaagct taaacctaag tttataaatt
      721 aaagtttata tacttaaatg ataatttttt ctatctctaa cttctatctc taacataaaa
      781 aatattaaac ataaaactaa taacttagtt aatgaaataa ctaaattact gaaaggacta
      841 aaagtaaatg gctaaaatac ttcttttgga agatgatgat acaattacat ttggaataaa
      901 aacttccctt gaaaagaaaa atcacactgt atgttgttat aatacgatta ctgaagctaa
      961 aaaaaatttt gataaaacta ttgatttaat tctgcttgat ttaaatctac ctgatggtat
     1021 tggatacgag ttttgccaat atgttaaaag tatagaagat actccagtga tatttttgac
     1081 agtccgtgat gatgaaaaag acattatacg tggattagat atgggtgcag atgactatgt
     1141 ggttaaacca tttttgcttt cagtacttca atccagaatt aatgcagttc ttagaagaac
     1201 tcattccaat actaacaata ctattacatg tggaaatata agcttaaata aagttgaagt
     1261 caaagcatat ttagataatg ttgaagtacc tttgacagca ggagaatata agcttcttct
     1321 aagtcttatg gaaaataaaa atagagctat gactcgtaca aagttactag aaatgctctg
     1381 ggatgtagat ggtaatttcg taaatgacaa cactcttact gttacaataa agcgtcttag
     1441 agaaaaatta aaaaatacac cttgtataaa aacattaaga ggcataggat atagaatgga
     1501 ggacagctag tgaaaactcc aagaaatatc aacagtacag ttgtttttat aatattctgc
     1561 ttaattgtaa cttttttatt gagtgtaatt ctaacaacta taacttcttt taatgaatac
     1621 aatgtactgt ctaaggctct tggtgcatca tatgagtaca atcctaagtc tacagaaagt
     1681 atactaaaat cattgaaaaa ttctagtgat gaggatttta aaacaggaaa tgagatttta
     1741 tcaaaacatg gatattctaa aagtacattt tggagtaaaa acttttttta ttttctagtg
     1801 gtatcgtttg gtataatttc aacaatgact atagttttat atataatcaa acatagaaca
     1861 aataagcaaa aattgagtag aataaacaat cttactcaat acttagaaga agtgaattta
     1921 ggaaaagagt ctgtactttt acgatgtgaa gatgaatttt ctcaccttga agatgagata
     1981 tataaaacag ttggggaact tcgttgttca aaagaatttg ctttaaaaga aagacaaact
     2041 ctttcagata accttgcaga tattgctcat caacttaaga ctccaattac ttctatgtca
     2101 cttatggcac aattactttc agaaaattgt ccagatgaag aaatagatta tattaacagg
     2161 cttacaaatc aaatctcaag acttgagaga ctagtttcat ctttacttac attatcaaaa
     2221 ttggatacaa gtactttaat atttgaaagc aaacttatag atgtacattc tcttcttact
     2281 tatgcaattg agcctataga aggttcttta agagaaaaac accagacatt tactatcgaa
     2341 ggttctcctt cggtagaatt tgaaatcgat attaattgga ctttagaagc tcttttaaat
     2401 attcttaaaa attgtagtga gcatatagaa gatggtggat atatagttgc ttattatagt
     2461 caaaatcctc tatatgttga gattatcata gaagataatg gtaatgggtt tgctcaggaa
     2521 gaacttccat atatttttaa tagattttat cgtggtaaaa atgctagtaa ggacagtatt
     2581 ggtataggac ttgctctatc aaagtctatt atagaacgtc aaaatggtac tctacatgca
     2641 gaaaattgcc ataatggtgg tgcaagattc ataattaaat tttacacata aaatgctgga
     2701 ttataggtaa aatacctata atccagccat atctatattc tatgttatct attattttag
     2761 taatatactt ctatttacat ttaagatttt attaaataaa caacttaact atccctatac
     2821 ttgtaagtag tcttaaatcg aaacccttaa ataagtccaa atagcttttg tttatattgt
     2881 ttcatttttt atagattcta ctatattttc attcttcaac tgcttgtatc ctgtcatata
     2941 tgatgctatt acaataaaaa gtatcaatat ggtaaaacct attattgtag atacaggtgc
     3001 aaatggtaaa tactctataa aatatatttc atttatacgt agaaaagtat acactattcc
     3061 tatttgcaca ggaatactaa gtagtaatgg tgtaattcct aagaacaaac cttccaaaac
     3121 aagcatcttt tttatgccat ctggagataa tccaacagaa cgcaacatag caaactcttg
     3181 tcgtctactt ctaatatttc ctgacactgt agcaagtaca tttgataatc caatcatagc
     3241 caataaacct gtaataaagc ctactatcaa attcatggtc tttcttccac tagcatcttg
     3301 tttttcactt tctaaaacat cctcaatact atagtctccc gaaccatagt ctttattaca
     3361 aattgactct aatttttctc ttactctaga aatgtcctta cgattattta ctttaaataa
     3421 tcctataagc ctatttgaac gtaacctact tacttcatca taatttttag atatttcctg
     3481 caccatagac attggcacta tttgcattaa tgtatatctt gaactagtat ctactatttt
     3541 aggaagtgtg cttgtaatag ctcccacttt catgttgtaa gtataatccc ctttatcttc
     3601 gtcatatacc ttttcattta atttaatgtt atctcctatt gaaatactta aataatctat
     3661 atacacttta tttctacgtg tgctacgatt aacatcttcc tccttattat atacaattga
     3721 ggttggattt tttgtatcat aaaataattt tgggtctaca gaaatttgtc tgcaatattc
     3781 tccaaaactc ttatcatcaa gcccaattat atttgaaatt atacgaaatt tgccatcacg
     3841 ttctatagga gagtattttt tagatgaaac tatactttta aatcctccat ttttagcaag
     3901 ttcttttgaa gcatcttttt cagtcagcca agtggcagca gacatttttt gataatatag
     3961 tgagctcttt acaccttcta tattatctaa tttttcaata aaagtagact ctacaggttc
     4021 actattttcc aaataaactg ctatatctct ttggtcttta taattatcac ttccataaac
     4081 agcttttgct ccttcttgat tggcaattat atgcaaaaaa cctgtaagta ataaaaaaga
     4141 taatgttaaa gatattgttg cagtacgata agatttttta cgagcacgta gtgcatttga
     4201 tgcaagttct ccttctattc caaatatctt tcctaatgaa tatttatgtg actttttgat
     4261 tttaacacta tctccttgtc gtattgcctc tataggagat atttttgata cttttcgtgc
     4321 tggtataagt gcagaaatcc agactgttac tattgtaagt aaaactgctg gcagaaaagc
     4381 aggaatacca taagtaaata ccacttctgg agcatcagta tgataattaa ctgaattaat
     4441 atagactata agcctattac acaatagcca tcccaaaaat aaacctattg gtaatggtat
     4501 aattgtcagt aaaaagcctt caaacactac agacctttta atttgttttg gtgatgcacc
     4561 aacactagat aatatcccta attgagtaag tctagcattt gcagacagtg aaaatgcatt
     4621 gtaaatcacc attacaaaca cggctactgt aaaaattgct attgtaagat acataatagg
     4681 cgttgaaaat tttgatatac tactcataga tgctttctgt tctggggaaa agactaacat
     4741 ctttaaaaga taattactat tgtatttaag attgtatttt ccatattcat tagtcttcca
     4801 ccccaatgat ttagcaagtt ttggaagttc cttatacgta tctcttatat ttttaaatcg
     4861 caaatacaca gttatactat cattaggttt tatacttgtt ttatcaagat agccaagagc
     4921 tgtatatgca ggtattactg atgaagtaga aacatctacc tttcctacta ttgtcaatgt
     4981 agtctctgaa atttttttaa aactttcccc tttcgatttt ggggctatgg gttcaagagg
     5041 ctttccattt aggattctat ctcctattgg gagagtaatt ttatcaccaa tttttacact
     5101 tggattattc tcaaaatatt gttttgatag tgcaatttca ttagtattac ttggtcgacg
     5161 accctctatt atagtgtctt tttctggcat atgattccaa tactctgatg tagcatttct
     5221 ataaattata taatctcttt tgtcattatt aattttgcct acataccatt gtccttttac
     5281 cattacatca tcaactgaag aaaaattttc aattaacgat aagtcttttc cataagtatc
     5341 gtcaaacaat tcaccatgcc agttaccttc tttctcaatt gaaagtctta taaaatcagt
     5401 ccacatagta tacaacagtc cacacaagga agacatcatt gttgtagtaa gtaatatagc
     5461 caacattata gctatactac tacgtttatt gcgtcgtaca aaatctaagg tgtattcttg
     5521 aagaatattc atttcttcac caccttttca gagacaatca ctccatcttc catagttaca
     5581 atacgatttg cctctagtgc tattttctca tcatgagtaa taagaagtat tgtctgatta
     5641 aatcttttat ttgataagtt taacaaatct acaatttctt ctgaattttt tctatcgaga
     5701 ttaccagttg gctcatctgc taataaaata gctggtctat agattaaact tctagctata
     5761 gatacccttt gttgttgtcc acctgaaagt tgacttggaa gatggtttaa tctatcagat
     5821 aatcccaata tagatacaat ctcagagaat ctatcctcat ccacctttct tttatcaagt
     5881 aacatgggta ataatatatt ttttctaaca tcaagagtag gtataagatt ataaaattga
     5941 taaataagac ctacttttct acgtctaaaa actgcaagag cttcttcctt taaacttgat
     6001 atttctgtat cttcaacata aatctttcca gatgtaggtc tatctactcc cccaagcata
     6061 tgtagcaatg tagatttacc agaaccagat gctcctacaa tggatacaaa atcacctttt
     6121 tgaactgata aatttacatt attaagggct gtcactctgg tttgatttga accatatatt
     6181 tttgtgagat tttcacattt taatatttcc atatacaatc atctccttta tggttaaatt
     6241 ttagcaacct aaaatgacat tatagtgaca taaatataaa tagcgattac tattaaaaat
     6301 attgtttagt ttttgcacat tatttaaaat tataatataa aacaaaaact atgcaatata
     6361 ttttatttat acttttaata tattacatag tctttaaaag atattaactt taaatctata
     6421 ggtatttaat ttaataatca atagcaagta ttttatgtac taaaggagtt ttgctctata
     6481 tagactcaat ttagtaaaat tatttaataa atattacaga ctataggtaa ccaatattta
     6541 caaataaaat taagtttatt cccttacact ataatatttc ttaattatat taattgcaat
     6601 ttcctttacc tttggccctg taatttcttt attggaatca tctgatttaa tagcaaaata
     6661 ataggtatca ccttcacgct ctagcatacc aacaaaccat gcatccacac gtttattttt
     6721 ttcatcaaat cctgttccag tttttccata tacatttatg tttttatcat ttacatctat
     6781 tttcataata tcttttaaaa tattaatatg ctctttttta aagtttgtat ctccctcaaa
     6841 tatttttgcc attgtttgca cttgttcttt tggagatatt tgtaatgaag attctaacca
     6901 aaatccattt aaatgtccct ttccattttt taaatctcct tcccattcac taatatcgca
     6961 attcccatac tttaaatcat ctaaaatatt ttggacagat ttagcatcaa ctttatttat
     7021 caattttttg tagtaccaaa cacaagactc cttaaatgcc tcttctaaac taagattttt
     7081 attccaattt ttatttggat aatttgtacc atcatacccc ataacggatt cttttgagtt
     7141 tattacccct ttttctaaac caattagagt tgaaactatt ttaaaagtag aacatggtga
     7201 ccttcttgtt tcaattaatt ctttgttata tatattatat tctttatttt tagtattata
     7261 aaaaatagct cctccactaa taccttcaaa acaatcacta tagtcaacta tattaatttt
     7321 ttcttttttg tttttatcat tgttttcaat tttagattta tttaattcag agtttatata
     7381 attcttatca tatttttgtt ttgtattgtt atgctttctt cctacatagt atattcccat
     7441 agctacaaca actagaagaa taactatcca aataaaatta ttttttcttt tcatacttta
     7501 taccatctcc tctacataag tttatagttc acctttaaaa taatatacat tatttgtaat
     7561 atactccaat ctaggagctt gaatacttct gtttataaaa tccaactttg ttttaacatg
     7621 ttttaagtct ttaaaaatca caataccatc aatagattgt aattcagctc caaagaaaaa
     7681 atagactaaa tttctatccc cagaaatttt aactacgtct aaatttctgt ttagtttttt
     7741 catttttttt attacttctt tagaaaatat cttctctaat tcatttttat cagattgaat
     7801 ataaaagaca cattcagagc ttaacttttt ttcatcataa actttcaaaa tagctttagc
     7861 tactacacta taatcttttt cataaagttt aaatctaaaa ctttcacttg gactaggaat
     7921 aaatatagtc cctatactta taaagatact aaatatacat ccaatcattt ttcttttatt
     7981 taaacctttt ttatagtaaa gcttaatcat atgtacacaa atccatataa atgcaattag
     8041 catcaatggt attgcaaata aacctataaa tagtagcatt actcctcccc tacatattat
     8101 atttccacac aaaaataaaa aatcacttac aataaataga gttaaaggga taactatatc
     8161 tcttatattt tctttaccta atataattat gttgattgag gctatgatga atatactcaa
     8221 tataactatt agtatcatta tgtcctcctt tgagtttgtg aggtaattat ttgttcttta
     8281 tttaaaactt actattgtaa tatttaaata ttacaatagt aagttttaaa tgtcaagtag
     8341 ttttaattgt ataattatct ttgtacttat taaattacga gtatctatta tatacttttt
     8401 tatctactat caactactat ttattcagat tgtatgggat tggttaaatt tagaaatact
     8461 ttctataaca tagcagaata tatcaagaaa gtagatttta attttattat aatatggttg
     8521 cataatataa ttgctttact aaaatgtact cagaaaggat ttaacattca tgtcaaatat
     8581 tataataaaa ggacttacac aaaataacct aaaaaatata tcatttaaaa ttcctaaaaa
     8641 taaaattgtt gtttttactg gagtttctgg ctctggaaaa tccagtatcg tatttgatac
     8701 aattgcagca gaatcacaga ggcagatgaa tgaaacatat tcttcattta tcagaaatag
     8761 gctacctaaa tatagaaaac ctcaggtaga gctgattgat aatttaactg cttctgtaat
     8821 cgtagaccaa actccccttg gcggcaatgc acgttctaca gttggtacta taagtgattt
     8881 gtattcatca ttaagattat tattttctag aattggagaa ccctacatag gaacaacttc
     8941 acatttttct tttaatgacc caaatggtat gtgtaaaaat tgttctggga ttggtagagt
     9001 taaagtaatc aatattgaat ctattataaa tcctgaaaat tcatggaata caggatgtat
     9061 aaatgactct ttatttaaac ctaattcatg gtactggaag cagtatgcag agtctggact
     9121 ttttgattta gataaaccta taaaagatta ttcttttgaa gaatataatt tattgcttta
     9181 tggttcaaaa aataaagatg gaaaacaaga aaatccaaaa atagatggta tttttaacaa
     9241 atacaatcaa acatatctaa atcgggatat tagtaatatg agcaaacaca caaaagaaaa
     9301 atcagaaaga cttataacag ataaagaatg tcctgtatgt catggaaaaa gattaaataa
     9361 agaaactctt aactgtaata ttttaggata ttcgattgca gatatgtgca atatggaact
     9421 tactgacctt tatgatttat tgttacaaat aaaagataaa agaactgaaa ctgtaataaa
     9481 aactcttgct gatggtttaa aaagaatgat tgatattgga ctcccttacc tacatctaaa
     9541 tagagaatca tcatctctat ctggaggaga agctcaaaga ttaaaacttg tacgctatat
     9601 ggggagtagt ctcacaggta tgacttatat tttcgatgaa cctagtactg gtatgcatcc
     9661 acgagatgta catcgtatga atcagttatt aaaaaaactt agagatagag gaaacactgt
     9721 ccttgtagta gagcatgata aagatgttat ctccatagca gatgaagtta tagatattgg
     9781 cccaagtgct ggcacacagg gtggatatgt tgtattccaa ggctcttatg ataaattact
     9841 tttagcaaac actcttactt caaatgcaat gaatctatca ttacctataa aaactaactc
     9901 tcgtcatcca aaaggtaccc ttccaattag agatgcttgc ataaataatc taaaacatat
     9961 tgatgtagat ttaccagttg gaattatgac tgttgttact ggagtagcag gttctggcaa
    10021 gagtacttta atctctcgtg tttttgcaca aaaatacgaa aaagatgtta ttaaaatcga
    10081 ccaaagccca attacagcta caaaccgttc tatgcctgct acatttcttg gtttctttga
    10141 tgagataaga aaagtatttg ctaaagaaaa taatgttgaa gaaggtttat tcagctttaa
    10201 ttcaaaagga gcttgtcctg tttgtgatgg taaaggagta cttgttacag aacttgtatt
    10261 catggaccct attattaata catgtgaagc ttgcaaaggt actagatata gtgaagaagt
    10321 tttgtcaaaa ttatataaag gcaaatgtat agttgatgta ctatctatga cagtagatga
    10381 agctcttgaa ttttttacag aaaagaagct atgtaaaatc ttacaaacaa tgaaagatgt
    10441 tggtttacct tatataacct taggtcaacc acttagtaca ctatctggag gagaacgtca
    10501 acgaattaaa ttagctaaaa acctcaacaa aaaaggtact atatatattt tagatgagcc
    10561 gactacaggg cttcatggtt ctgatattga aaagcttatg ttgatttttg agcatatagt
    10621 tgataaaggt aatactgtaa ttataattga acataatttg gatgtattga aacaagcaga
    10681 ttgggtaatt gatattggtc ctgatggtgg caaaaatggt ggtcaggtcg tatttgaagg
    10741 tacaccacta gaaatgtttg aaagaggttg cactataaca tcagatttcc ttagaaaatc
    10801 aataaagata tagataatat aatactaata ttaaaaaatt aag
//
LOCUS       DAEPXF010000131        10753 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain HB27
            SAMN13376389-rid12479423.denovo.138, whole genome shotgun sequence.
ACCESSION   DAEPXF010000131 DAEPXF010000000
VERSION     DAEPXF010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13376389
            Sequence Read Archive: SRR10518432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10753)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10753)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001069624.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001069624.1
            Genome Coverage       :: 315x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/15/2021 01:54:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,165
            CDSs (total)                      :: 4,076
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 89
            rRNAs                             :: 10, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 10 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 19 of 69
            Pseudo Genes (incomplete)         :: 44 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 10 of 69
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10753
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13376389-rid12479423.denovo.138"
                     /strain="HB27"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="China: Hebei"
                     /collection_date="2017"
     gene            63..827
                     /locus_tag="KRL67_002810"
     CDS             63..827
                     /locus_tag="KRL67_002810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoaP domain-containing protein"
                     /protein_id="HBH1523676.1"
                     /translation="MQIIDININNLDNEHICCSISEKKGENCVFSKKTWMKNNFNKGL
                     VFKRFDVRGKAFIEYIPAENAWCPITADNYMFINCFWISGKFKGQGYSNLLLQECIDD
                     AKSKGKCGLVVLSSSKKMPFLSDAKYLKYKGFCLADTLEPNYELLYFPFIDNASIPCF
                     KNCVKQRKLNENGIVLYYSNQCPHTEKYALILKKIANFRGIDVNLIKFETAKEAQNAP
                     APFTTYSLFFNGEFVTSEILSEKKIEKFLDTNIKKI"
     misc_binding    1047..1260
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            1514..2548
                     /locus_tag="KRL67_002811"
     CDS             1514..2548
                     /locus_tag="KRL67_002811"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HBH1523677.1"
                     /translation="MNKIKVGIIGATGYVGAELIRLLINHDKVEVTAIGSNSYVGKDI
                     VDIYPSIGYKNNMICIENEKVIDMCDVVFTALPHGVSEKFVIKAIKSKKKVIDLGADF
                     RIKDEEVYSKWYGVSFIDKILHKKAVYGLSEIYKEDIKDADIIANPGCYPTSISLPLM
                     PLLSSKLIKNNNIIIDSKSGLTGAGRELSISSHFTEVNENITAYKIGKHRHTPEIEQN
                     LSESCKEKVSVVFTPNLISVNRGILSTIYCTKEDNISINDIHRKLTDYYEFKEFIEVL
                     PLDKVASLKNVRFSNKCVISLHENGDTLIICSAIDNMIKGAAGQAIQNMNIIFGIEEN
                     TGLKNIAPSF"
     gene            2589..3806
                     /gene="argJ"
                     /locus_tag="KRL67_002812"
     CDS             2589..3806
                     /gene="argJ"
                     /locus_tag="KRL67_002812"
                     /EC_number="2.3.1.1"
                     /EC_number="2.3.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate
                     N-acetyltransferase/amino-acid acetyltransferase ArgJ"
                     /protein_id="HBH1523678.1"
                     /translation="MEIIKGGVTVSEGFFASGIHCGLRKNKEKRDLALVYSDVLCDAA
                     AVVTQNKVKGNPVYVTQEHIKNGKAQAIIVNSANANTFNGKEGLINAYKMAKFTSDKL
                     NIKENDVLVASTGVIGKPLNIDLIEENMDELVNNLSKQGHIGAREAIMTTDIIKKEIA
                     VAIMYGDKKITIGGMAKGSGMIHPNMATTLGFITTDANIDGVLLKEALKIAVDKSFNR
                     VSVDGDTSTNDMVMILANGKAKNDRINKKDEHYQVFLSALTYVCIELAKLVAKDGEGC
                     TKLIECYINGALSEEHAVKLAKTVISSSLVKTAVFGADANWGRILCALGYAGEEIDME
                     KVDIIFESMKGYIEVCKNGNGLDFNEEKAKKILEDDEISILVDLNMGNARGNAWGCDL
                     SYDYVRINGSYRN"
     gene            3821..4681
                     /gene="argB"
                     /locus_tag="KRL67_002813"
     CDS             3821..4681
                     /gene="argB"
                     /locus_tag="KRL67_002813"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HBH1523679.1"
                     /translation="MINIEKANTLIEALPYIEKHQGKTIVVKYGGSAMKKDGLKESVM
                     EDLVLMSYVGINIVLVHGGGAEINKMLAKVDIESKFVNGLRYTDEETMEIVKMVLAGK
                     VNKDLVNKIHTKGGKAVGLCGIDNNMILCDPYKNYELGFVGEIKKVNVELIESCLKSG
                     YISVIATIGVGDDGETYNINGDTAASAIAKELNADKLILLTDVPGLLREPDEEKSLIT
                     EVILEDVDKLFEEGIITGGMIPKIQGCVDALNNGVNRVHILDGRVPHSIITELFTDSG
                     IGTLIRKENE"
     gene            4711..5898
                     /locus_tag="KRL67_002814"
     CDS             4711..5898
                     /locus_tag="KRL67_002814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HBH1523680.1"
                     /translation="MNNNNTISKWNEYFIDTYNQPNFVIDYGEGSCFFDTNGNKYIDF
                     TSGYGVSSLGYSNGNLKNALKEQVDKLLHTSNLYFNEPVLYSGEKIINSSGMAKVYFC
                     NSGTEANETAFKIARKYSSDKYGNGRGTIISLKDSFHGRTMMSLMATGMDKYHKYFYP
                     LPEGFKYIERNNIEDLKNNIDSTVCAIILEAIQGEGGVNVLEKDYVLELVKICQEKDI
                     VVIFDEVQCGIGRTGKLFGYEYFDVKPDIVTVAKGLGAGIPVGGVLVNKKLSKVLGKG
                     DQGTTFGGNLLAMVATSVVLDEISKDGFYNEVLEKGNYIRKSIESFNNKVVLKTKGIG
                     LMIGIETNIESSIIEEKARKKGLLILTAGKNVLRFLPPLTISYKEIDEALEILKDILL
                     EIN"
     gene            6008..7030
                     /gene="argF"
                     /locus_tag="KRL67_002815"
     CDS             6008..7030
                     /gene="argF"
                     /locus_tag="KRL67_002815"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HBH1523681.1"
                     /translation="MNNISLKGKSFLTLKDFTKEEIRYLLTLSSYLKTKKKVGIKGDL
                     LQGKNIALLFEKTSTRTRCSFEVAAHDEGAHVTYLGPNDCHMGKKESVSDTAKVLGRF
                     YDGIEFRGFKQETVESLAKYSGVPVWNGLTDEYHPTQILADFLTTIENVDKDLNKVKF
                     VYVGDARNNMGNSLMIGCAKMGIHFVALAPKELWPNEELVKEMKEIAKKSKGDIMLTE
                     NIDDVKGADVIYTDVWVSMGEEEQYETRINQLKDYQVNMDMIKKTENENVIFLHCLPA
                     FHDLETKVAQEIYEKFGLSELEVTNEVFNSKYSKVFEEAENRMHTIKAVMVSTMTDVN
                     MNNNLI"
     gene            7157..7774
                     /locus_tag="KRL67_002816"
     CDS             7157..7774
                     /locus_tag="KRL67_002816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4878 domain-containing protein"
                     /protein_id="HBH1523682.1"
                     /translation="MKKIILALTTFLLAVSLTACSSAKPEDTIDSFFNSAKKFDFEGM
                     NKVMENNDKKYKDILKELDTKDPNAQYVLDYLKQNASKITYTIKDSEVKGDKATIKVE
                     CKFIDSTPLLQEIVAEAFTKMLGMSFSGQDLTDEKTTEMLVSIMKEKQKSVKETYVTK
                     TVEFKCSKKDNKWIISSVNDVVADVLLSNLVTAGQEFSNSMNALE"
     gene            complement(7992..8690)
                     /locus_tag="KRL67_002817"
     CDS             complement(7992..8690)
                     /locus_tag="KRL67_002817"
                     /EC_number="5.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid racemase"
                     /protein_id="HBH1523683.1"
                     /translation="MNEKLIGILAGMGPRSTAPFVDLIIDECQSQYGAKYDDEFPKMM
                     IYSLPTPFYIDRPINHELMKETIIDGLQKLESTGVNFIAMPCNSAHIYFKELKESINI
                     PLLNIVEETVKKLPTLSQKVTLFSTSSTFESTIYQNGIIHNGHEFIFKDEWQIKLNTL
                     IQNIKIDKENPHNIDIWNKLIEDVKNESIENIVIACTDLNVVLEKSYPSINIVDSSKC
                     LAESVVNKYLKLVK"
     gene            complement(8852..10069)
                     /locus_tag="KRL67_002818"
     CDS             complement(8852..10069)
                     /locus_tag="KRL67_002818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallo-hydrolase"
                     /protein_id="HBH1523684.1"
                     /translation="MLGKKCMDYLQVLGKISSTTNGLTRLILTQEHKKSIDLISSWMK
                     GLNLDIEIDDIGNVIGTYKSSFPNAPTLVVASHQDSVKCGGIFDGMLGIIVPLVGLEE
                     AKHNNKSYPFNIKLIAFAEEEGTRFETSLMGSKVFAGTFKEELLKSVDENGITLEEAV
                     TKFGFNTKNLTNLHPRKDVDAYLEFHIEQGPVLENESLPAGIVSSITGFKSFKISVNG
                     KSGHAGTLPMNMRLDAGCCACECVLAIEKVAKTTADLVATVGKMNFYPSSSNVVPERA
                     EFTLDVRSCSQEILDNSVEKIFNEISHICENRKLNYTSELAFENVPVPCSNKITKIIE
                     KSFIDLNLNPFYIYSGAGHDAQEMDNITDIGMVFIRCAGGVSHNPNESVSVDDLDTAV
                     KIFLKILDNLDLK"
     gene            complement(10261..10674)
                     /locus_tag="KRL67_002819"
     CDS             complement(10261..10674)
                     /locus_tag="KRL67_002819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBH1523685.1"
                     /translation="MELEITEKIEKQDEDTIFQGLLKYNLARLEDKNPVDLGIYIRDK
                     AGKIVAGFIGVTHGNWLSIKYLWVSEKLRYKGTGSQLLYKAEKIAKERACKYVFLDTF
                     SFQAPKFYEKFGYHKVFSLENYPLTGKRYYFRKIL"
BASE COUNT         4206 a         1177 c         1809 g         3561 t
ORIGIN      
        1 taaaataaaa aatatataga atatatttaa aaattattat gtaattagaa aaggagataa
       61 gtatgcagat tattgatata aatataaata atcttgacaa tgaacatata tgttgttcaa
      121 tttctgaaaa aaaaggagaa aattgtgttt tttctaaaaa aacttggatg aaaaataatt
      181 ttaataaagg tttagtcttt aaacgatttg atgtaagggg taaagctttt atagaatata
      241 ttccagcaga aaatgcatgg tgtcctatta ctgcagacaa ttatatgttt atcaactgct
      301 tttggatatc tggaaagttt aagggacaag gatattctaa tctattgtta caagagtgta
      361 ttgatgatgc taaatcaaaa ggtaaatgtg gattagttgt gctttcgtct agtaaaaaaa
      421 tgcctttttt atctgatgct aaatatctaa aatataaagg cttttgttta gctgacacat
      481 tagaaccaaa ttatgagcta ttatattttc catttattga taatgcttct attccatgtt
      541 ttaagaattg tgtaaaacaa agaaagttaa atgagaatgg tatagtttta tattatagta
      601 atcagtgtcc acatacagaa aaatatgctc ttattttgaa aaaaattgca aattttagag
      661 gtattgatgt aaatcttata aaattcgaaa cagcaaaaga agcacaaaat gctcctgcac
      721 catttactac atacagtctt ttttttaatg gtgaatttgt aaccagtgag atactttctg
      781 agaaaaagat tgagaaattt ttagatacca acattaaaaa aatttaacta aaaagagatg
      841 taaatattgt attttgagag tatattattt tataatctaa tatatgggaa ttttttaagt
      901 tgatgaatga taatataaaa attataactc aatttagata caaaatgctg gaaattaacc
      961 atatagaaaa tatttccaaa aattttaaaa ttatattgac aaagttaaaa aaataaacta
     1021 tcatataaaa caataagaaa ataaattaaa atcctgtgaa aaagagagta gcataaaact
     1081 ggttgataga gagctgggaa tggtggaagc ctagtaacga attttattgt gaatagagct
     1141 ttggaggagg ttaccagaag taatagtatg gtaattggtg aatctcgcct taagagatta
     1201 gagtgggtaa gattatctta tcaattagag tggtaacgcg ggtgtatatt cgtctcttgg
     1261 tctttgacca ggaggctttt ttaattttaa atatacattt tgtagatact ttttaatttg
     1321 agttttaatg attattgtaa ttttaccaat aagagtggta ccgcggaaaa tcgcctctta
     1381 gtatttataa tgctaagagg ttttttgttt taattagaat attttaatct acgtattatc
     1441 aaatgattat catatagtaa agtggaaagg tagaatttta agactatttg aatataaaaa
     1501 tggagggaac aaaatgaata aaataaaagt tggtattatt ggtgcaactg gatatgtcgg
     1561 ggcagagttg attagattat taataaatca tgataaagtc gaagtgacag cgataggttc
     1621 aaattcgtat gttggaaaag atatagttga tatatatcca agtataggat ataaaaataa
     1681 catgatatgt atagaaaatg aaaaagttat agatatgtgt gatgttgtgt ttacagccct
     1741 tccacatgga gttagtgaaa agtttgtaat aaaggcaatt aaatcaaaaa agaaagttat
     1801 agatttaggt gctgacttta gaataaaaga tgaagaagta tatagtaaat ggtatggagt
     1861 atcctttata gataaaatct tgcataaaaa agctgtatat ggattaagtg aaatatacaa
     1921 agaagatata aaagatgcag atataattgc aaatcctgga tgttatccaa ctagtatttc
     1981 tttaccattg atgccattac taagttctaa attgataaaa aataataata taataattga
     2041 ttctaaatca ggtcttacag gtgctggtag agaattatcg attagtagtc actttacgga
     2101 ggttaatgaa aatatcactg catataaaat agggaaacac agacatactc cagaaataga
     2161 acaaaattta agtgaatcct gcaaagaaaa agtaagtgta gtttttacac caaacttaat
     2221 ctcagtaaat aggggaatct tatcaactat atattgtaca aaagaagata atatttctat
     2281 aaatgatata cataggaaat taactgatta ttatgaattt aaagaattta tagaagttct
     2341 tccattagat aaggtagctt ctttaaagaa tgttaggttc tcaaataaat gtgtaatatc
     2401 attacatgaa aatggagata cattgataat ctgttcagct atagataata tgattaaggg
     2461 agcagcaggt caagctatac aaaacatgaa tataatattt ggtattgaag aaaatacagg
     2521 tttaaaaaat attgcaccat cattttagaa ataaaaaata ctaaactcat ataattagga
     2581 ggataaatat ggagattata aagggtggag ttacagtttc agaaggattt tttgcttcag
     2641 ggattcactg tggcttgaga aaaaataaag aaaaaagaga tttagctttg gtttattcag
     2701 atgttttatg tgatgcagca gcagtagtaa ctcaaaataa agtaaaggga aatcctgttt
     2761 atgttacaca ggaacacatt aaaaatggaa aagcacaagc tatcatagta aatagtgcta
     2821 atgctaatac ctttaatgga aaagaaggac ttataaatgc ttataaaatg gctaaattca
     2881 ccagtgataa attaaatatt aaagaaaatg atgttttggt tgcatctaca ggtgttatag
     2941 gaaagccatt aaatatagat ttaatagaag aaaatatgga tgaattagtg aataatctaa
     3001 gtaaacaagg tcatattgga gcaagagaag ctattatgac aactgatatt ataaaaaaag
     3061 aaatagcagt agcaataatg tatggagata aaaaaataac tattggaggt atggcaaaag
     3121 gttctggcat gatacatcct aatatggcaa caacgttagg atttataact acagatgcaa
     3181 atattgatgg agttttgcta aaagaagctt tgaaaattgc tgttgataaa agttttaata
     3241 gagttagtgt agatggagat acaagtacta atgatatggt gatgattcta gcaaatggaa
     3301 aagcaaaaaa tgatagaata aacaaaaaag atgaacatta tcaagtattt ttgagtgcac
     3361 ttacatatgt ctgtatagaa cttgctaaac ttgttgcaaa agatggagaa ggatgtacaa
     3421 aactaattga atgctatata aatggagcat taagtgaaga acatgcagta aaattagcaa
     3481 aaacagttat atcttcttca ctagtaaaga cagctgtatt tggagctgat gcaaattggg
     3541 gcagaatatt gtgtgcttta ggttatgcag gtgaagaaat agatatggaa aaagttgata
     3601 ttatttttga gagtatgaaa ggatatattg aagtatgtaa aaatggaaat ggattagatt
     3661 ttaatgagga aaaagcaaaa aaaatccttg aagatgacga aataagtata ttagtagatt
     3721 taaacatggg caatgccaga ggaaatgcat ggggatgtga tttaagctat gactatgtaa
     3781 gaataaatgg aagttataga aattaggagg atatacttta atgattaata tagaaaaagc
     3841 aaatacactt atagaagctt taccatatat agaaaaacat cagggaaaga ctattgtagt
     3901 taagtatggt ggaagtgcaa tgaaaaaaga tggtctaaaa gaatctgtta tggaagacct
     3961 tgtgttaatg agttatgttg gaatcaatat agttttagta catggtggag gggcagaaat
     4021 caataagatg ctagcaaaag tggatataga aagtaaattt gtaaatggtc ttagatatac
     4081 agatgaagag accatggaaa ttgtaaaaat ggtgcttgct ggaaaggtta ataaagacct
     4141 agtaaataaa atacatacaa aaggtggaaa agcagtaggg ctttgtggaa tagataataa
     4201 tatgatttta tgtgacccat ataaaaatta tgaacttgga tttgtaggag aaatcaaaaa
     4261 agtaaatgtt gagcttatag aatcttgtct aaagtcaggt tatatttcag taattgcaac
     4321 tataggtgtt ggagatgatg gagagactta taatataaat ggtgacacag cagcttctgc
     4381 tatagctaaa gagttaaatg cagataaatt gatacttctt acagatgtcc ctggtttgtt
     4441 aagagagcca gatgaagaaa aatcattaat aacagaagtt atattagagg atgtcgataa
     4501 attatttgaa gaaggtataa ttactggagg tatgatacct aaaatacagg gatgcgtaga
     4561 tgccttaaat aacggagtaa atagggtaca tattttagat ggaagagttc cacattctat
     4621 aataacagag ttatttactg atagtggaat aggtacatta ataagaaaag aaaatgaata
     4681 aaattataag tttaagaggg gagaataaga atgaataaca acaatacaat ttcaaaatgg
     4741 aacgaatatt ttatagatac atataaccaa cctaattttg tcatagatta tggtgaagga
     4801 agttgttttt ttgatactaa tggcaataaa tacatagatt ttacaagtgg atatggagta
     4861 tcttctcttg ggtatagtaa cggtaattta aagaatgctc taaaagagca agtagataaa
     4921 ttattacata cttcaaattt atattttaat gagccagtgt tgtattcagg agagaaaata
     4981 attaattcaa gtggaatggc taaagtatat ttttgtaatt ctggaacaga agcaaatgaa
     5041 acagcattca aaatagcaag aaagtatagt agtgataaat atggtaatgg tagaggaact
     5101 atcataagtt taaaagactc ttttcatggt agaacaatga tgtctttaat ggcaactggt
     5161 atggacaaat accataagta cttttatcca cttcccgaag gttttaaata tatagaaaga
     5221 aataatattg aagatttaaa aaataatatt gattcgacag tttgtgcaat aattcttgaa
     5281 gcgattcaag gcgaaggtgg agttaatgtt cttgaaaaag attatgtttt ggaactagta
     5341 aaaatatgtc aagaaaagga tattgtagtg atttttgatg aagtccaatg cggtatagga
     5401 agaactggga aactttttgg ctatgaatat tttgatgtaa aaccagatat agttaccgta
     5461 gcaaaagggt tgggagcagg aataccagta ggaggagtac tagtaaataa aaaactttca
     5521 aaagtactag gaaaaggaga ccaaggtaca acttttggag gtaatttatt agctatggtg
     5581 gcaacttcag ttgtattaga tgagatatct aaagatggtt tttataatga agtattagaa
     5641 aaaggaaatt atataagaaa atctattgaa agttttaata ataaagttgt attaaagaca
     5701 aaaggtattg gtctaatgat aggaattgaa actaatattg aatctagtat tatagaagag
     5761 aaagctagaa aaaaaggatt gcttatcttg actgctggaa aaaatgtatt gagattttta
     5821 ccaccattaa caatcagtta taaggaaata gatgaggctc ttgaaatcct aaaagatata
     5881 ttactagaga ttaactaaat attcgaaaaa tagtttaatt aaagacaaaa attagaagta
     5941 aataaaaaag taaagtgtta acaagtttaa ttgaatttaa aaagtttata aaaaggggcg
     6001 aattaatatg aataatataa gtttaaaagg gaagagtttt ttgacattaa aggattttac
     6061 aaaagaagag attagatatt tattaacttt atcaagttat ttaaaaacta agaaaaaagt
     6121 tggtataaaa ggcgatttgc tacaaggaaa aaatatagct ttattatttg agaaaacatc
     6181 aacaagaact agatgttcat ttgaagtagc agctcatgat gaaggagcac atgtaactta
     6241 tctaggtcca aatgattgtc atatgggtaa aaaagagtct gtatcagata cagcaaaagt
     6301 tctaggaaga ttctatgatg gaatagaatt tagaggcttt aaacaagaaa cagttgaaag
     6361 tctagcaaaa tattctggtg tacctgtttg gaatggactt acagatgaat atcatccaac
     6421 acaaatactg gcagactttt taacaacaat tgaaaatgta gataaagatt tgaataaagt
     6481 taaatttgta tatgttggtg atgctagaaa taatatggga aactcactga tgataggatg
     6541 tgctaaaatg ggaatccatt ttgtagcact agcacctaag gagctttggc caaatgaaga
     6601 gttagttaag gaaatgaagg aaatagccaa gaaatctaaa ggagatataa tgttaactga
     6661 aaatattgat gatgttaaag gtgcagatgt tatatataca gatgtatggg tttctatggg
     6721 agaagaggag caatatgaaa caagaataaa tcaacttaaa gattatcaag taaatatgga
     6781 tatgataaag aaaactgaaa atgaaaatgt tatattttta cactgtcttc cagcatttca
     6841 tgatttagaa actaaggttg ctcaggagat ttatgaaaag ttcggattat cagaattaga
     6901 ggttacaaac gaggtattta attcaaaata ttcaaaggta tttgaagaag ctgaaaatag
     6961 aatgcataca ataaaagctg ttatggtttc tactatgact gatgtaaata tgaacaataa
     7021 tttaatatag acatgtttta atatggattt tactttaatg aaaatttata aaatatttta
     7081 aatatattat tcaaaaactc tcattatatg ttaaaataac ttatgtatca acaaattaag
     7141 agggaggatt tgagatatga aaaaaatcat attagcttta acaacttttt tattagcagt
     7201 atctttaaca gcatgttcaa gtgcaaaacc agaagataca atcgatagtt tttttaacag
     7261 tgcgaagaag tttgattttg aaggcatgaa taaggtaatg gaaaataatg acaaaaaata
     7321 taaggatata ttaaaggaat tagatacgaa agacccaaat gcccaatatg ttttagatta
     7381 tctaaaacag aatgcatcta aaatcacata tacaataaaa gacagcgagg ttaaaggtga
     7441 taaggctaca atcaaagtag aatgtaaatt tatagattca acaccattac ttcaagaaat
     7501 agtagctgag gcatttacaa agatgcttgg tatgagtttt tcaggacaag acttaactga
     7561 tgagaaaaca actgaaatgt tagtatcaat tatgaaagaa aaacaaaaaa gtgttaaaga
     7621 gacttatgtt acaaaaactg tagagtttaa atgttctaaa aaggataata aatggataat
     7681 aagttcagta aatgatgtag tagctgatgt attgttatct aatcttgtta cagcaggaca
     7741 agaattttct aactctatga atgcacttga ataatattgt ttaggtgtag taaagtaata
     7801 aatatatgat tatttttata aataaacatg taaatattta tgaaggatac tatttttggt
     7861 atccttttta ttaatatact gaaatacatg gttaatttct atattgtcaa gaaactatga
     7921 tatatgttat aagtctatac tatttaagat aaacttataa catatatttt ttatagtata
     7981 gtgcatgaaa tttatttaac taattttaga tatttattga caactgattc agccaagcat
     8041 tttgatgaat caacaatatt tattgaagga tatgattttt ctaaaactac atttaaatca
     8101 gtacaagcga ttactatatt ttctatagat tcatttttaa catcttcaat aagtttattc
     8161 caaatatcta tattatgtgg attttcttta tctattttta tattttgaat aagagtgttt
     8221 agttttattt gccattcatc tttaaatata aattcatgtc cattatgtat tataccattc
     8281 tggtaaattg tagactcaaa agttgaacta gtcgaaaaaa gagttacttt ttgtgagaga
     8341 gtaggtaatt ttttaactgt ttcttctaca atatttaata gtggtatatt aatagactct
     8401 tttaattctt taaagtatat atgtgctgaa ttgcatggca ttgcaataaa atttacacca
     8461 gttgattcaa gcttttgtaa tccatcaatt atagtttctt tcataagttc atgatttatt
     8521 ggacggtcaa tataaaatgg ggtaggtaat gaatatatca tcatctttgg aaattcatca
     8581 tcatattttg ctccatattg agattgacac tcatcaataa ttaaatcaac aaaaggggct
     8641 gttgaccttg gacccattcc agccaaaata ccaattaatt tttcattcat aaaaattatc
     8701 tcctttacat ataaatatat ttatgtataa aataaaaatc tataaaagca taaaattata
     8761 ttttataata gattatatta tacacttgta taatataaat ataaagtgag tttttaaatt
     8821 atatatttta atatactttc ttatataaaa attattttaa atcaagatta tctagtattt
     8881 ttaaaaatat ttttacagcg gtatccaaat catctacaga aacactttca ttaggattgt
     8941 gacttacacc cccagcgcat cttataaata ccataccaat atcagttata ttatccattt
     9001 cctgagcatc atgaccagca ccactataaa tataaaaagg attaagattt aaatctataa
     9061 aacttttttc tatgattttt gttattttat tagaacatgg tacaggaaca ttttcaaaag
     9121 caagttcact tgtataatta agttttctat tttcacatat atgtgatatt tcattaaata
     9181 tcttttctac agaattatcg agaatttctt gtgaacaaga gcgtacatca agagtaaatt
     9241 cagctctttc aggtaccaca tttgatgaag atggatagaa attcatcttt cctacagtag
     9301 caaccaaatc agcagtcgtt ttagctactt tttcgatagc taatacacat tcacaggcac
     9361 aacatccagc atcaagtctc atattcattg gaagtgtacc agcatgtcca gacttaccat
     9421 ttactgatat cttaaaactt ttaaatcctg taatacttga tacaattcca gcaggaagag
     9481 attcattttc taaaacagga ccctgctcga tatgaaattc taaatatgca tctacatctt
     9541 ttctaggatg caaatttgtt aagttttttg tattaaatcc aaactttgtt acagcttctt
     9601 caagtgtaat tccattttca tcaacagatt taagaagttc ctctttaaaa gtaccagcaa
     9661 aaactttact tcccattaaa gaagtttcaa atctagttcc ttcctcttca gcgaaagcaa
     9721 ttagcttaat attgaaagga taagacttat tattgtgttt tgcttcttca agaccaacta
     9781 gaggcactat tatacctaac ataccatcaa atatacctcc acactttaca ctatcttgat
     9841 gagatgcaac tactaatgtg ggagcatttg gaaagcttga tttatatgta cctataacat
     9901 ttccaatgtc atcaatttca atatcaagat ttaaaccttt catccatgaa ctaattaaat
     9961 caatagattt tttatgttct tgagtcaaga taagtcttgt aagaccattt gttgttgaac
    10021 ttatttttcc taatacttgt agataatcca tacatttttt tcctaacata tttacctcct
    10081 aaaaatataa tttttattta agctaagatt agcttttata cttataattt ttgaactttc
    10141 aaataatttt gtatgtatag taaaatacta ttttagtatt aataatgata taagaacata
    10201 aagtctgtag acgaagagca gaagttttaa gttaaaaaat attaaatgtg atttttatta
    10261 ttatagtatt tttctaaaat aatatctctt tccagttaga ggataatttt caagtgaaaa
    10321 tactttatgg tatccaaact tctcataaaa tttaggtgct tgaaaactga atgtatctaa
    10381 aaaaacatat ttacaagctc gttctttagc aattttttca gccttatata aaagttgact
    10441 tccagtacct ttatatctaa gcttctcact aacccataag tattttatag aaagccaatt
    10501 accatgagta acacctataa aacctgcaac aattttacct gctttatctc gaatatatat
    10561 tcctaaatcc actggatttt tatcttcaag tctagcaaga ttatatttta ataatccttg
    10621 aaaaatagta tcttcatctt gtttttctat tttttctgta atttctaatt ccatatttga
    10681 actttctcct tgtataatct tttgcatatt ggtatttttc aataaaatca gtagaaagaa
    10741 tatagtttaa cat
//
LOCUS       DAEPXF010000132        10752 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain HB27
            SAMN13376389-rid12479423.denovo.139, whole genome shotgun sequence.
ACCESSION   DAEPXF010000132 DAEPXF010000000
VERSION     DAEPXF010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13376389
            Sequence Read Archive: SRR10518432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10752)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10752)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001069624.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001069624.1
            Genome Coverage       :: 315x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/15/2021 01:54:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,165
            CDSs (total)                      :: 4,076
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 89
            rRNAs                             :: 10, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 10 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 19 of 69
            Pseudo Genes (incomplete)         :: 44 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 10 of 69
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10752
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13376389-rid12479423.denovo.139"
                     /strain="HB27"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="China: Hebei"
                     /collection_date="2017"
     gene            <1..1084
                     /locus_tag="KRL67_002820"
     CDS             <1..1084
                     /locus_tag="KRL67_002820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HBH1523686.1"
                     /translation="ALSTVSTLLVYLIEQLVYGFTAPLNYLILISDINTDIYKFDINM
                     GLSSLITIIIAGFIYWYSSKKFNIKVMNFDKYLAILMIPLLLIILFMQSFEYSSNINI
                     DTSLGIVKLLLNTSITEEIQAFLFSIIGTICVFFSLFTFNKLIQALEDDKERAIMNQQ
                     IHAQKNYIEEAKSRLSQTISFRHDFNNHLAIVNGLLKKDQILKAQDYLNKLEKVTSDL
                     SFSCNTQNIVIDALFNNKLSIAKQLGIEVDCEVIIPQITNMNDFDLCIVFANAIDNAI
                     KACRVVDKQNRYLKLSTKLEGGFFMIEIKNSYNPINNYTSGSGIGLLNIKEIAKKYQG
                     AISIDKTSDYFQINVLLVISLHCASI"
     gene            1123..1608
                     /locus_tag="KRL67_002821"
     CDS             1123..1608
                     /locus_tag="KRL67_002821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH1523687.1"
                     /translation="MYLNRESLIYGNEEVTKMKVGNINGQAPITNGLDSCQSEVQGLE
                     QKKKALQDEIEKVRSDKSIDGETAKLKIEALKKEIEDIENKIQELKGNKAKQTNKEEN
                     NVSEVNNNLLERKKEPSNNENVKQLYDVFERSSADEQWDNTYRVLQDDKNLKVKFNRP
                     I"
     gene            complement(2243..2467)
                     /locus_tag="KRL67_002822"
     CDS             complement(2243..2467)
                     /locus_tag="KRL67_002822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HBH1523688.1"
                     /translation="MTVYNLKLGQKGIIDNIAGNEKLMKRLLALGLIDGTEVEVKKIA
                     PLNDPIVIRFRGFDLAIRKSDAKNINLKNN"
     gene            complement(2564..3817)
                     /locus_tag="KRL67_002823"
     CDS             complement(2564..3817)
                     /locus_tag="KRL67_002823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:glutamate symporter"
                     /protein_id="HBH1523689.1"
                     /translation="MITLTFDIVQTLTLSIFFFLIGNLLKNKVNFLNNFCIPAPVIGG
                     LLFCFLNLFFKYFNIADISISGNLMPNFITFFFTTIGLEISINLIKKGGSVLFRYWIL
                     CGVLAFCQNILAITISKIIKLEPLLGLMCGNVSMEGGHGYSAAFGLTIENLGIEGAVG
                     VGLSAATIGLIMGGILGCPVAKFLINKYKLKPSSNIDLSIPRYNRNLKRFGSKFFRNK
                     NRIKNNNFSQTITPSIFLEQVLLIFICINTGEIISRCFYITFNILLPSVVTCMFSAVI
                     FRNLNDKINILELNFKLIDFLKELSLGIFLTLSLMNIDLFELSTLLPPILLIVTFQVI
                     FIILFSIFICFRVLGKDFDSAIIISGLIGHGIGATPNALANMSSLTQKYGASPKAFLV
                     VPLVSGFLLDAISIPCILFFINILT"
     gene            4077..5066
                     /locus_tag="KRL67_002824"
     CDS             4077..5066
                     /locus_tag="KRL67_002824"
                     /EC_number="3.5.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HBH1523690.1"
                     /translation="MFENLPKIDLHCHLDGSVRVETMLDIAIKEKIDLPSNNMDEIKK
                     LAKVSFNCTSLDEYLEKFDLPLKVMQSKENLKRITFELLEDASRENVKYIEIRFAPLL
                     HTQKGMSVKNIIEGIIEGIREAESIYDIKGNLILGCMRTMTSKEALLVIEEGKSFVNK
                     GVVAVDLCGPEKEGFCKEYKDVFKLAREYGYKVTIHAGEAASGENVLDAINILKADRI
                     GHGVKIKDHKKAYNLVKDKKILLELCPTSNVQTKTVDSYEAHPFYTFYKDNLHVSINT
                     DNRTVSDISLSSELNVIFDTFKLGLEDYKIIYRNAVEASFADKETKEYLNSLI"
     gene            5138..5878
                     /locus_tag="KRL67_002825"
     CDS             5138..5878
                     /locus_tag="KRL67_002825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EcsC family protein"
                     /protein_id="HBH1523691.1"
                     /translation="MKDYEDVVYIKLIKKELEEWKKNILKKPSIFDKASKGIQNKFNG
                     VLPDKYHEIITSSIKVMTKTVLFGYKYATKPPLRNMSLEEREKLVLEKAKTYKATAMI
                     EGAGTGAGGIFLGLSDFPLLLGIKVKFLYDVASIYGYDVKDYRERIYILNILQLAFSS
                     QQHINKTFKLMNNWDEYINSLPEDINDFDWRTFQQEYRDYLDLAKFLQLMPGIGAFVG
                     FYVNGKLIDKLCETAVSAYRLRIRELKL"
     gene            6087..7238
                     /locus_tag="KRL67_002826"
     CDS             6087..7238
                     /locus_tag="KRL67_002826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacyl-CoA dehydratase"
                     /protein_id="HBH1523692.1"
                     /translation="MTNLPKQFDSFNEARQKGFINAKELKESGKKMVGVFCTFTPVEI
                     PMAAGATVVGVCGVSEEPIPDAERVLPRNLCPLIKSSYGHAITDTCPYFYFSDLLIGE
                     TTCDGKKKMYEELAKVKPTYVMHLPNTSKGEFAYKLWKDEMIRLKEEVEKSLGVTITE
                     EDIRAAIKDKNEERELLKEFYALGKLQPSALTGLELHNVLYQAGFKFDRAELKHSLRK
                     VIDDMKERYEKGECPVQKDKPRILITGSPIGGISEKIVKTLEDAGASVVAYELCGAIR
                     SNDLLVDEEIEDVYDALTQKYINIGCSCMMNNDNRIELLDRIIDEYNVDAVIDVVLQA
                     CHTFNIESYRIREFVTKEKNKPFMSLETDYSKSDTEQLRTRFEAFVEML"
     gene            7263..8018
                     /locus_tag="KRL67_002827"
     CDS             7263..8018
                     /locus_tag="KRL67_002827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyglutaryl-CoA dehydratase"
                     /protein_id="HBH1523693.1"
                     /translation="MFSIGVDSGSVATKGVLFDGEKIIKKIIIPTGWSPKSTSKQVYE
                     LLSSEIDKKYIKKVVGTGYGRGVMDFADKKVTEITCHTRGVYFLNKNIRTILDVGGQD
                     SKVINLDRDGNVFNFIMNDKCAAGTGRFLEITSNLLGSDIESIDTLAKGYEPVNISSM
                     CTVFAESEIVSLLAQNISTGEVAAGILKSIANKSASMLARGEVIDEVAFTGGLAKSKE
                     LVKMIEEILGKKIFIAEDTQIIGALGAAVIGFR"
     gene            8211..10598
                     /locus_tag="KRL67_002828"
     CDS             8211..10598
                     /locus_tag="KRL67_002828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding cysteine protease Cwp13"
                     /protein_id="HBH1523694.1"
                     /translation="MKKFTSKKVARFVALFLISILVMSTIPVSADNSNTLNQIKKAEY
                     SDTYKQYLNDAKNGRTEKYNGIIPNPYQLEGTQIQSKGRTAPTSYDPRKLGLMTSVKN
                     QEDLGICWDFAAMATLESFLKLNNYGDYDLSEEHLRWWASDGEYGWSVNDMDGALNYE
                     AMGYLTSWSGPKLEKDIPYNGRVSKVQGAKKPTNMNTAPTVFNVTDAVCVSNDINSTK
                     NAILQYGAVTSGYYEDIKYQSDDQNSYYCPTKSFNTNHAISIVGWDDNYSKDNFNANI
                     RPSKNGAWLIKNSWGDYNSEGGYFWISYEDKTLMSDIDNFSIKGGKKPNDDEKMYQHD
                     YASIVPLISKKITAANVFDFNRGDETLKSVMFLTESIGAKYEVYYAPVVNGIPQENNM
                     KKLKEGTAQYSGYITVPIDSFNIPEGKGAIVVSIEGKNGESTIGSESNVPGYDTFKAK
                     ANLGESYIIDNTGKFFDINRDSNFYPCNFTIKAVTEKSSGESIPNESLIGSDRYETAI
                     KVSQNGFNSSENVVLVNGSSIVDALAATPFTSAINSPILLTQKEALNSKTKAEIQRLG
                     AKKVYLIGGENSISKEIEQQLKSLNISIERISGSDRYKTSLLLAQKLNGIKNVSQIAV
                     VNGVKGLADAISVGAAAAENNIPIILANEKSELQGADEFLNSLNIEKSYIIGGTASLS
                     NNLESKLKNPTRLSGSSRDETNSNIIDNFYKKDTLKNAFVVKNGIKSQNDLIDGLAVG
                     PLGAKTGSPVILVGDKLADSQKEVLKNKTLEKVTQVGGGANQNAFKELVKLKSAK"
BASE COUNT         4299 a         1193 c         1783 g         3477 t
ORIGIN      
        1 tgctttatct acagtatcaa ctttgttagt atatttaatt gaacaattag tatatggatt
       61 tacagctcca ttaaattact tgatacttat tagtgacata aatacagata tttataaatt
      121 tgatataaac atgggactat cttctttaat tactattata atagctggat ttatatattg
      181 gtatagttct aaaaagttta atattaaagt tatgaacttt gataaatatc tagcaatttt
      241 gatgattcca ttgttgctta taatattatt tatgcaatca tttgaatata gttcaaatat
      301 aaatatagat acaagtcttg ggattgtaaa gctgctatta aatacttcta taactgaaga
      361 aattcaagct tttctttttt ctataattgg aactatatgt gtattttttt ctctatttac
      421 ctttaataaa ctaattcaag ctctcgaaga tgacaaggag agggcaatta tgaatcaaca
      481 gattcatgct caaaaaaact atattgaaga agcgaaatca cgattatcac aaaccatatc
      541 ctttagacat gattttaata atcatttagc tattgtcaat ggattgttga aaaaagatca
      601 aattttaaaa gcacaagatt atttaaataa gcttgaaaaa gtaacaagtg acctatcatt
      661 ttcttgtaat acacagaata ttgttattga tgctcttttc aacaataaat taagtatagc
      721 aaaacaatta ggcatagagg tagattgtga agtaattata ccccaaatca ctaacatgaa
      781 cgattttgac ctatgtatag tgtttgctaa tgctattgac aatgcaatta aagcttgtag
      841 agttgtagat aaacaaaata ggtatttaaa gctttccact aagttagagg gtggattttt
      901 tatgatagaa ataaaaaata gctataatcc aataaataat tatacaagtg gttcaggtat
      961 aggtctttta aatataaaag aaattgctaa aaaatatcaa ggggctataa gtatagataa
     1021 gacatcagac tattttcaaa taaatgtatt gctagttatt tcattacatt gcgctagtat
     1081 ttgagaacaa tttcattgaa aatagtattt gtaagctata tcatgtattt aaatagagaa
     1141 agtttgatat atggaaatga ggaggttact aaaatgaaag ttggaaatat aaatggtcaa
     1201 gcaccaatta caaatggttt agatagttgt caaagtgagg tacaaggctt agaacaaaag
     1261 aaaaaagcat tacaggatga aatagagaaa gtaagaagtg ataaaagtat tgatggtgaa
     1321 acggccaaat taaaaataga agctttaaaa aaagaaatag aagatattga aaataaaatt
     1381 caagagttaa aaggaaataa agcaaaacaa acaaataaag aagaaaataa cgtttcagaa
     1441 gtaaataata atctattaga gagaaaaaaa gaaccttcta ataatgaaaa tgtaaaacaa
     1501 ttatatgatg tttttgaaag aagcagtgca gacgaacaat gggataatac ttatcgtgtt
     1561 ctccaagatg ataaaaactt aaaagtaaaa tttaatagac ctatatagtt tatttataaa
     1621 aataattaat attttaatta tcatcacttt ttagaagaat acacatattt atttacagtt
     1681 agcacattta tatgtggttt tataactatt atttacaagt ctatattgat aacttttaca
     1741 tagtatataa tgtttaaata cttattcaat tttatctata cataaacaaa aaaagagatt
     1801 ttaaaatctc tttttagatt gttgacttat ggctttttga tttaataaaa tcaagaagcc
     1861 gtttgaattt tatctgttgg ctgataccct tctgatattg aaacaccatt tttgtttcca
     1921 aaatcgctta aaattaagaa cttattatga atgactattt cattatgaac aataagcaaa
     1981 tgacctgaat cttcaatcaa tgttttaaca gtagcattaa atgtttgctt atacagttct
     2041 atcgtttggt tgaactgacc attaaagtaa atatgagtca ataacataac tatatctaca
     2101 aaaattaata taaatttttt atactgtaca ttatctaact gttgattatc tgtattacaa
     2161 catgattgaa tttaaaaagc catcaacaca tagaatgtcg atggcctgaa aatagatttt
     2221 aaaatctctt ttttattata tattaattat tttttaaatt tatatttttt gcatcagatt
     2281 ttctaatagc taagtcaaac cctctaaatc taatcactat agggtcatta agaggagcta
     2341 tctttttcac ttcaacttca gtgccatcta ttaatcctaa ggctagaagt ctcttcataa
     2401 gtttttcatt tccagctata ttgtctataa ttcccttttg tccaagtttc aagttgtaaa
     2461 cagtcattat taacacctcc taatgaaaat cattatcaaa ctttatattt ataatatata
     2521 acaaaaagag caaattgtca atatataaca aaaaataaac atactaagtt agtatgttta
     2581 taaaaaatag tatacatggg atactaatag catctagcaa aaaaccactt actaatggta
     2641 caaccaaaaa ggcttttgga gaagcaccat atttttgtgt taaagaactc atatttgcca
     2701 aagcgttcgg agtagctcct attccatgtc caatcaaacc acttattatg atggcagaat
     2761 caaaatcttt tccaagtact ctaaagcaaa taaatattga aaataggatt ataaagatta
     2821 cttgaaaagt tactattaag agaataggag gtaataatgt agacaattca aataaatcta
     2881 tattcataag cgataaagtt aaaaaaattc ctaatgaaag ttcttttaaa aaatctataa
     2941 gtttaaaatt taattctagt atatttatct tatcatttaa atttctaaaa ataacagctg
     3001 aaaacataca agttacaaca gagggtaata gtatattaaa cgttatatag aagcatctac
     3061 ttataatttc tcctgtattt atacaaataa atatgagaag aacctgttcc aaaaatatag
     3121 atggtgttat tgtttgggag aagttattat tttttatcct attcttattt ctaaaaaatt
     3181 tacttccaaa tctctttaga tttctattat atctaggaat cgacaagtct atatttgatg
     3241 atggttttaa tttatattta tttattagaa atttagctac aggacatccc aatataccac
     3301 ccataataag tcctattgtt gcagcagata atccaacccc aactgctcct tctataccga
     3361 gattttctat tgtaagtcca aatgcagcag aataaccatg accaccttcc atagatacat
     3421 ttccacacat aagccctaaa agaggttcaa gttttataat ctttgatatt gtaatagcta
     3481 atatattttg acaaaatgcc aatactccac acaaaatcca atatctaaat aatacactac
     3541 cacctttctt tataagattt atgcttattt caagtcctat agttgtaaaa aagaaggtta
     3601 taaaatttgg cattaaattt ccacttatag atatatctgc tatattaaaa tatttaaaaa
     3661 ataaatttag aaaacaaaat aatagacctc ctattactgg tgcaggtata cagaaattgt
     3721 ttaaaaagtt aactttattc ttcaataaat taccaattaa aaagaagaat atagatagtg
     3781 tcaaagtttg cactatatca aaagttaagg taatcaaaat aagtcctcct ttgtaattca
     3841 aagttttaat tatataactt aaattcttat gtattgaaat tttattatca aatgtatatt
     3901 aataaatgta tattaaaaag aatcagtaga tataattaga taatcataaa ttaaattaaa
     3961 atattttaat gagatgtaac ttttttagaa tgaaaaaccg tagtatattg atataataga
     4021 aataagactt aaaaatctgt ttatagtatt atttataagt acagtaggag gataaaatgt
     4081 ttgaaaattt acctaagata gatttacatt gccacttaga tggaagtgtt agagtagaaa
     4141 ctatgcttga tatagctatt aaagagaaaa tagatttacc ttcaaataat atggatgaaa
     4201 taaaaaaatt agcaaaggtt tcttttaatt gtacttcact agatgaatat ttagaaaaat
     4261 ttgatttacc cttaaaagta atgcaatcta aggaaaattt aaaaagaatt acttttgaac
     4321 ttttggaaga tgcaagtaga gaaaatgtaa aatatattga aattagattt gcaccattac
     4381 ttcacaccca aaaaggtatg agtgttaaaa atataattga gggtataatt gaaggaatta
     4441 gagaagctga aagtatttat gatataaaag gcaatttaat attaggttgt atgagaacaa
     4501 tgacctcaaa agaggcatta ttagtaattg aagaaggaaa atcatttgta aataaaggtg
     4561 ttgtagcagt agacttgtgt ggtccagaga aagagggatt ttgtaaggaa tataaagatg
     4621 tttttaaatt agcaagagaa tatggatata aagttacaat acatgcagga gaagctgcaa
     4681 gtggagaaaa tgtattggat gctataaata ttttaaaggc ggataggatt ggtcatgggg
     4741 taaaaattaa agaccataaa aaggcgtata atttagttaa agataaaaaa atattgcttg
     4801 aattatgtcc aactagtaat gtacaaacta agacagtaga ttcttatgaa gcacatccat
     4861 tttatacttt ttataaagat aatttacatg taagtataaa tactgataat agaactgtat
     4921 cagatataag tttaagttca gaattaaatg ttatatttga tacttttaaa ctaggattag
     4981 aagactataa aataatttat agaaatgcag tagaagctag ttttgcagat aaagaaacta
     5041 aagagtattt gaatagttta atttaataga taaaaatatt taaactttaa attaagctat
     5101 taaattgaca aaaatacatt gtattaggag aaaaaatgtg aaagattatg aagatgttgt
     5161 ttatattaaa ctaatcaaaa aagaacttga agaatggaag aaaaatatat taaagaaacc
     5221 ttctatattt gacaaagcat caaagggtat acagaataaa tttaatggag tattgccaga
     5281 taaatatcat gaaataatta cttcttctat aaaagtaatg acaaaaaccg tattgtttgg
     5341 atataagtat gctacaaagc caccacttag aaatatgtca ttagaagaaa gagaaaaatt
     5401 agttttagaa aaagctaaaa cttacaaagc cacagcaatg attgaaggag caggaacagg
     5461 ggcaggagga atcttcttag gtttatctga ttttcctttg cttttaggta ttaaagtgaa
     5521 atttttatat gatgtagctt caatatatgg atatgatgtt aaagattata gagaaaggat
     5581 ttatatatta aacatattac agttggcttt ttctagtcaa cagcatataa acaaaacttt
     5641 taaattgatg aataactggg atgaatatat aaatagtcta ccagaagaca tcaatgattt
     5701 tgattggaga acatttcaac aagagtatag agattattta gatttagcta agtttttaca
     5761 actaatgcct ggaataggag catttgtggg attttatgtg aatggaaaat taattgataa
     5821 attatgtgaa actgctgtgt ctgcttatag attgaggatt cgagagctta agttataaaa
     5881 gttataagag aagttataac ataaaaaatt agttatacaa tatatctata tacatttaat
     5941 tttgcattga taaaaacaat tatggataaa aggtaaagca tgatagaatc taatgtagag
     6001 gtttgtattt taatggaaaa atttagtttt tattgtttaa gatacaaatt gaattatgaa
     6061 gagaggtaat tgggaggaat attttcatga caaatttgcc aaaacaattt gatagcttca
     6121 atgaggctag acaaaaagga tttataaatg caaaggaact taaggaaagt ggaaaaaaga
     6181 tggttggagt tttctgtaca tttactccag ttgaaattcc aatggcagct ggagcaactg
     6241 tagtaggtgt atgtggtgtt agtgaagagc caatacctga tgcagaaagg gtgcttccaa
     6301 gaaatctttg tcctttaatt aaatcaagct atggacatgc aataacagat acctgtcctt
     6361 atttctattt ttctgatttg ttgataggag aaacgacttg tgatggtaag aaaaaaatgt
     6421 atgaagaact tgcaaaagtt aagccaacat atgtaatgca tttgccaaat acctcaaaag
     6481 gagaatttgc ctataaactt tggaaagatg aaatgataag gttaaaagaa gaggttgaga
     6541 aatctttagg tgtaactata actgaagaag atataagagc tgctataaaa gataaaaatg
     6601 aagaaagaga acttttaaaa gagttttatg cacttggtaa attacaacca tctgcactta
     6661 caggccttga gttacataat gttttatatc aagcaggttt taaatttgac cgtgcagaac
     6721 ttaaacattc tttaagaaaa gttatagatg atatgaaaga aagatatgaa aaaggtgaat
     6781 gtcctgttca aaaggataag ccaagaatat tgataacagg ttctcctatt ggtggaataa
     6841 gcgaaaaaat agttaaaact ttagaagatg caggggcatc agtcgttgca tatgaacttt
     6901 gtggagctat taggtcaaac gatttattgg ttgatgaaga aatagaagat gtatatgatg
     6961 ctttaacaca aaaatatata aatataggtt gttcttgtat gatgaataat gataatcgta
     7021 ttgaattatt agatagaata atagatgaat ataatgtaga tgctgtaata gatgtagttt
     7081 tacaagcatg tcatacattt aatatagaaa gttatagaat aagagaattt gttacaaaag
     7141 aaaaaaataa gccatttatg tctcttgaaa ctgattattc aaaatcagat actgaacaat
     7201 taagaactag atttgaagcg tttgtagaga tgttgtagga ataaataaaa gtaggaggaa
     7261 ctatgtttag tataggagtg gactcagggt cagtggcaac aaagggtgtg ttatttgatg
     7321 gtgaaaaaat tattaagaaa ataattatac caactggatg gagccctaaa agtacatcta
     7381 aacaggtcta tgagttgcta agtagtgaaa ttgataaaaa atatataaaa aaggtggtag
     7441 gtacaggtta tggaagggga gtaatggatt ttgctgataa gaaagtaaca gaaataactt
     7501 gtcatactag aggcgtttac tttttaaaca agaacataag gactatttta gatgtaggtg
     7561 gtcaagatag taaagttata aatttagata gagatggaaa tgtatttaac tttataatga
     7621 atgataagtg tgctgcagga actggtcgat ttttggagat tacttctaat cttttaggaa
     7681 gcgatattga aagtatagat acattagcta aaggatatga accagttaat atatctagta
     7741 tgtgtacagt ttttgccgag tctgaaattg taagtcttct agctcaaaac atttctactg
     7801 gagaggtagc agcagggata cttaaatcta ttgcaaataa atccgcctca atgttagcaa
     7861 gaggtgaggt aatagatgaa gttgcattta ctggagggct tgcaaaaagt aaagagctag
     7921 taaaaatgat agaagaaata ctgggtaaaa aaatttttat tgcagaagat actcaaataa
     7981 taggagcttt aggagctgct gtaataggat ttagataaaa atatataaaa aagaaaatga
     8041 aaagctgaat ttatcataaa taagttcagt tttttatttt attttgttta tttataaagg
     8101 aaaactttgg tataattgat ttaaatgatt gtatatttgt aaatacttga tatttagtca
     8161 tatataacat tagaatttta aaaattagct caaaataagg gggagaaagc gtgaaaaaat
     8221 ttacttcaaa aaaagtagca aggtttgttg cactgttttt aatatctatt ttagtaatgt
     8281 caacaattcc tgtgtcagct gacaatagca atacattgaa tcagataaaa aaagcagaat
     8341 acagtgatac ttataaacaa tatctaaatg atgctaaaaa tggacgtaca gaaaaatata
     8401 atgggattat accaaatcca tatcagttgg aaggtactca aattcaaagt aaaggaagaa
     8461 cagctccaac atcttatgac cctagaaaat taggtcttat gacatcagta aaaaatcaag
     8521 aggatttagg aatatgctgg gattttgctg caatggcaac cttggaaagt ttcttgaagt
     8581 taaataatta tggagactac gatttatcag aagaacactt aagatggtgg gcttcagatg
     8641 gtgaatatgg atggtctgta aatgatatgg atggtgcttt aaattatgaa gctatgggat
     8701 atttaacatc ttggtcaggt cctaaattag aaaaagatat cccatacaat ggtagagtat
     8761 ctaaagtaca aggagcaaaa aaaccaacta atatgaatac agcacctact gtttttaatg
     8821 tgacagatgc tgtgtgtgta agtaatgaca taaattctac aaaaaatgct atattacagt
     8881 atggagcagt aacgtctggt tattatgaag atattaaata tcaaagtgat gaccaaaatt
     8941 catattattg tccaacaaaa agttttaata caaatcatgc tatttcaatt gtgggttggg
     9001 atgataacta ttctaaagat aattttaatg caaacattag accatcaaaa aatggtgctt
     9061 ggttaataaa aaatagctgg ggagattata attctgaggg tggatatttc tggatatctt
     9121 atgaagacaa gacacttatg tctgatatag ataatttttc aatcaaaggt ggtaaaaaac
     9181 caaatgatga tgagaagatg tatcaacatg actatgcaag tattgtacct ttaatttcaa
     9241 aaaagataac agctgcaaat gtatttgatt tcaatagagg cgatgaaact ctaaaatctg
     9301 taatgttctt aactgaaagt ataggagcta agtatgaagt atattatgct ccagtagtaa
     9361 atggtatacc tcaagaaaac aatatgaaaa aattaaaaga aggtactgcc caatattcag
     9421 gatacataac agttccaatt gattctttta atataccaga aggaaagggt gcaattgtag
     9481 tcagtataga ggggaaaaat ggagagtcaa ctataggttc tgagtcaaat gtaccaggat
     9541 atgatacatt taaagcaaaa gctaatttag gtgaaagtta tatcatagat aatactggaa
     9601 aattctttga tataaataga gatagtaact tctatccgtg taattttact ataaaagctg
     9661 ttacagaaaa gtcttctggt gagagtatcc caaatgagtc tttaattggt agtgatagat
     9721 atgaaactgc tatcaaagtt agtcaaaatg gtttcaattc ttcagaaaat gtagttttag
     9781 taaatggcag ttcaatagta gatgcattgg cagcaactcc ttttacatct gctataaatt
     9841 caccaatatt attgacacaa aaagaagctt taaattcaaa aactaaagct gagatacaga
     9901 gattaggtgc taagaaagta tacctaatag gcggagaaaa ttctataagt aaagaaattg
     9961 aacaacaatt aaaaagctta aatatatcga tagagagaat atcaggaagt gatagatata
    10021 agacaagttt attattagct caaaagttaa atggtataaa aaatgtatct caaatagcag
    10081 ttgtaaatgg agtgaaagga ttagctgatg caattagtgt aggtgcagca gcagcagaaa
    10141 ataacatacc aatcatactt gcaaatgaaa agagcgaatt gcaaggagct gatgaatttc
    10201 taaattcatt aaatatagag aaatcttata ttattggagg aacagcatcc ctatcaaaca
    10261 atctagaaag taagttaaaa aatccaacta gattatctgg tagtagtaga gatgaaacta
    10321 attcaaatat aatagataat ttttataaaa aagatacttt aaaaaatgca tttgtagtta
    10381 aaaatggaat caagagtcaa aatgatttaa tagatggttt ggcagttggt cctttaggag
    10441 caaaaactgg gtcaccagta attttagttg gtgataaatt agctgatagc caaaaagaag
    10501 ttcttaaaaa taagacttta gaaaaagtaa ctcaagttgg tgggggagca aatcaaaatg
    10561 catttaaaga gttagtaaaa ttaaaaagtg ctaaataggg tacttaagta ttcacatata
    10621 ctagtgtcta ttagtaactt atcttaataa aagctgaaaa taattacttt aaattattga
    10681 taaataagat aatcaatgat tgtaaagttt tgttttcagc tttaaattta tgtagtataa
    10741 gtttaaaaac at
//
LOCUS       DAEPXF010000133        10734 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain HB27
            SAMN13376389-rid12479423.denovo.140, whole genome shotgun sequence.
ACCESSION   DAEPXF010000133 DAEPXF010000000
VERSION     DAEPXF010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13376389
            Sequence Read Archive: SRR10518432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10734)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10734)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001069624.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001069624.1
            Genome Coverage       :: 315x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/15/2021 01:54:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,165
            CDSs (total)                      :: 4,076
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 89
            rRNAs                             :: 10, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 10 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 19 of 69
            Pseudo Genes (incomplete)         :: 44 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 10 of 69
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10734
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13376389-rid12479423.denovo.140"
                     /strain="HB27"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="China: Hebei"
                     /collection_date="2017"
     gene            221..1381
                     /locus_tag="KRL67_002829"
     CDS             221..1381
                     /locus_tag="KRL67_002829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF819 family protein"
                     /protein_id="HBH1523695.1"
                     /translation="MIETGFVYCSFLVVFVGVIFFMVDKLKWKVFDVINPMLLIYLGS
                     AILGSFGLFAQNEDVGTYQNIITVNFLPLMLVFLLIQCDLRKILKMGPKMLLSFFCAT
                     FTFMLGFVIAFIIFKNSMGADSWKILGAASGSWIGGSSNMIAAANALGVAEEGLSYAI
                     LMGSIGYTVWMSVCLMSVKFGPKFNKWTKCDTSFIDEVTSKLEKENKDNTAPTFVELM
                     ILLSVGFGVASFAQIVSKILPTSGILNETMWVVLVASTVSILLGMTKISKLKGSTLVG
                     NVFMYLLLAATGSKANFFGLSEAPVYIMFGLVVVLVHAISFIGLAKLFKLDLFTCEVG
                     SIANIGGVTTAPMIAGVHNPVLIPVGVLMGLLGNIIGTYCALMVTQILHMLA"
     gene            1724..1885
                     /locus_tag="KRL67_002830"
     CDS             1724..1885
                     /locus_tag="KRL67_002830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH1523696.1"
                     /translation="MIIPSRRIQDITLTNLKNGEVTLIELDEIYKKMGFLFVVSEGKL
                     KKIKKENHH"
     gene            2078..2707
                     /locus_tag="KRL67_002831"
     CDS             2078..2707
                     /locus_tag="KRL67_002831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HBH1523697.1"
                     /translation="MKIVTYNIHKGMDSNNRLTLTKMGLYLKQLDCDVICLQEVLYPQ
                     FLALKAVLNMDGVFATNVKKVNMIYGICTFTTFKMLNSNHFFLTSKKEQRGALCITID
                     AYGRIINVINTHLGLDRQERAKQLDEIIDYRNRLVGIVILCGDFNEKNVFLSMFNDMA
                     ISLNKSYLPTFEKSNSRIDYIFVNKNTELKGYTVEKIYLSDHYPVIGYI"
     gene            2947..4206
                     /locus_tag="KRL67_002832"
     CDS             2947..4206
                     /locus_tag="KRL67_002832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanW family protein"
                     /protein_id="HBH1523698.1"
                     /translation="MGRRATRVSRLGKEKKRSVLIVLAALVIIMGGFTYFFNSKFLYN
                     GKIAKNVYIEGVNVSDMTKAEALKAITDKYTPEDLNLTYDGKKYTISPKDIDLKYDTE
                     DVVKDAYESTKKGSYFQNLKKYIDIRVNKANMKIKAEYDEAKLSSKVSSIADSINVKM
                     KNASISVGSGGLSYTDSIVGREFDLAANKESIYNMIKNKEHKTLELKVNLQKPDITTE
                     QVKTVNSVIGQYSTTYSQAVEGRSYNVGLSARKTSDVLLMPGEEFSYNKLTGPSNKAN
                     GYKDAPVIVYGKLEQSAGGGVCQTSSTIYNAALLSGMEITQVTNHSSASTYVPKGRDA
                     TVSDGGLNLKFKNPYKHPVYIKNYAGGGSVSSVIYGNSGDKPNISIEVNQTGQNKYST
                     YRIFKDSSGKVIKKEHISNSSYKELKK"
     gene            4465..5460
                     /locus_tag="KRL67_002833"
     CDS             4465..5460
                     /locus_tag="KRL67_002833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HBH1523699.1"
                     /translation="MYEEVTWLNFIGNRYEVVNSDDVLEINKIYKARDVFYRKNVLIK
                     VIKHNNYICEDFVSNLIDESTALDEINSPYILKIIDVGIHCTEETTLYYVVSEDFNGI
                     GLDELILGNYLHLEAIVNIMIQVLKALEMIHRNHSYHGSLKSSSIIVDTEYNIKICDF
                     GITKANNGVNTRSYGNRRYLCPHQLCINYTDKESDFFATGLILFESIFKKLPFGESNS
                     EEKMLQSIDKGLDWNSIKAINGNTELINVIKRLLGRNNKYTRANDIIIDLSKVMYEKA
                     YIEVEDEKEEEQKQDKVIQVKKEYKSKVKKKLHKKLVVAGLAIVMISMIIISSII"
     gene            5706..6773
                     /locus_tag="KRL67_002834"
     CDS             5706..6773
                     /locus_tag="KRL67_002834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA ligase RtcB family protein"
                     /protein_id="HBH1523700.1"
                     /translation="MSNIKIIASNKCWIENIARKQLEDISKLNGILRVVGLPDLHAGK
                     IPVGLAVETKNIIYPHIIGNDIGCGMTLFKTGILKKKFKKDKWIKLLSKIKDLSDIEI
                     KNTYKEECPILNLGTIGSGNHFVEIQCISEIYNKEQFEQLKFSSDDIMMLVHCGSRNY
                     GEDILKKFYDKDGLEVESEKAIDYIKNHDNALIWAERNREAISKKIMQSIGTSGDVET
                     IFSINHNFIEKREDKFIHRKGAVSSERGAVIIPGSRGSLSYIVMPTENTEISLYSLSH
                     GAGRKWSRSVCKSRLKSKYSKDTIKQTKFKSQIICNDVNLLFQEAPQAYKNIEQIIES
                     LIEYELIQVVATMKPLITYKG"
     gene            7307..9217
                     /locus_tag="KRL67_002835"
     CDS             7307..9217
                     /locus_tag="KRL67_002835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HBH1523701.1"
                     /translation="MNSNTFERLQLNEVKELIQIHCVSSLGKNLIDKLTPSGNIGVVR
                     RKLAENKEARKIIENSNHIPLEGLFNAGSTIDKIEKGMIIEPIELVNIEDFLRGCRKM
                     KAFMLEKEFYSPTLSSYALNITECKSIEDEINYCIKSNKVDSNASKELKKVRRNIEIT
                     EGKIKDRLNKFITSTVNKKYIQEFIISKRNDRYVIPIKSSYKNEVNGTILDTSSKGNT
                     VFIEPISVSNLSTELTMLKADETIEEYKILSYLTELIFEKISQIKLNIEILSEYDMVF
                     AKAKYSQKIKGITPKINNNGYIKIIKGKHPLLTGDAVPLDFEIGKNYRSLIITGPNAG
                     GKTVTLKTVGLLTLMVQCGFDISAKEGSEFSVFEKVFVDIGDNQSIENALSTFSSHIK
                     NIAEIMSLSNNSTLVLFDEIGSGTEPNEGAGLAISLLEEFYKMGCITIASTHYGEIKK
                     FATLHPEFENAGMMFDKETLEPLYKLTIGKSEDSNALFISKKMGIKNRVLDRAKEYVI
                     DRNYNLDLVKRSKLQSNIEDKNSSEVILNYTDYNVGDKVKLLDEDDFGIVYKPMDKFN
                     NVEVLFNDNFISVNIKRLQLSIKASELYPEGYDLDVLFTSFKERKLEKDIQRGSKKTL
                     KKIQKEIKENRK"
     gene            complement(9280..9903)
                     /gene="ytaF"
                     /locus_tag="KRL67_002836"
     CDS             complement(9280..9903)
                     /gene="ytaF"
                     /locus_tag="KRL67_002836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation membrane protein YtaF"
                     /protein_id="HBH1523702.1"
                     /translation="MIESILLVTSLCIDAFVTSFGYGVNKVKVPLYSNIIISVVCSAI
                     LAISLFAGTIVRDFLPQNITMLICFFILFLLGSARLIESFFKSYFKKKSIHSKNYNFN
                     MLDLKLNFNVSIEPDVLDTSKSKVLKSAEAFTLALALSLDGLAIGFGSGLVGVNYIQV
                     ILFSLISNIIAVLFGCLLGNMFVKNINLNLSWLSGLILLVIGFMKIC"
     gene            10091..>10734
                     /locus_tag="KRL67_002837"
     CDS             10091..>10734
                     /locus_tag="KRL67_002837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBH1523703.1"
                     /translation="MEQHSKVGNFGDLLKILLEEKELSMGELSKKSGIDKSTISRIAN
                     NKQKPNINHLEKMAIHLNINLEELLKASGYEFRNSNNQIFNTDSDFSNFDDILGFANL
                     INDKNFNGNIEKELSKCKLYVQTDEGKKLLFDNFNKKIDSIEKQGQFTDRLRQMYADF
                     CTDGLTIKKYLLIGSMLLYFVISTDVIPDFVFPIGFMDDLVALNIVTKLLKNDK"
BASE COUNT         4408 a         1062 c         1743 g         3521 t
ORIGIN      
        1 aaaataattg ttgaaaaaga ttttaaattc agattattca tttctaaagt aattaatatg
       61 caaatcttta ttaatatatg tcaatagcta ggtatatatg tatttaatta gcatgatata
      121 gataaaagta tttattttaa ttgtctatta tctgaaaaag tctttactta aaaatcatta
      181 aaaaatcaaa ttaattagga aaagaaaggg tgtgtataat gtgattgaga ctggatttgt
      241 atattgtagt ttcttggttg tattcgtagg tgtaattttt tttatggttg ataaattgaa
      301 atggaaggta tttgatgtta ttaatccaat gcttttaata tatttaggtt cagctatact
      361 cggatcattt ggtttatttg cacagaatga agatgttgga acgtatcaaa atataataac
      421 agtgaacttc cttccattaa tgttagtatt cttgttaatt caatgtgatt taagaaaaat
      481 actaaagatg gggcctaaaa tgttattatc atttttctgt gctacattta catttatgct
      541 tggatttgtt atagcattca taatttttaa aaattcaatg ggtgcagatt catggaaaat
      601 tcttggggca gcatctggct catggatagg tggctcatca aatatgattg cagcagctaa
      661 tgctctagga gttgcagaag aaggacttag ttatgcaata cttatgggtt ctataggata
      721 tacagtatgg atgtctgttt gtctaatgtc tgtgaaattt ggtcctaaat ttaacaaatg
      781 gacaaagtgc gatacaagtt ttatagatga ggttacatca aaacttgaaa aagaaaataa
      841 agataataca gcacctacgt ttgtagaact tatgatttta ctttctgttg ggtttggagt
      901 tgcgtctttt gcacaaattg tatccaagat acttccaact tcaggtatac ttaatgaaac
      961 tatgtgggta gtacttgttg catctacagt atcaatatta ttgggtatga caaaaatatc
     1021 aaagcttaag gggagtacac tggtaggaaa tgtgtttatg tatttactcc ttgcagctac
     1081 agggagtaaa gcaaatttct ttggattgtc agaagcacct gtttacataa tgtttggatt
     1141 ggtagtagtc cttgtacatg ctatttcatt tatagggctt gctaaactat ttaaacttga
     1201 tttatttact tgtgaagtag gaagtatagc taatatagga ggagtaacta cagctccaat
     1261 gattgctggg gttcacaatc ctgtattaat accagtaggt gtacttatgg ggcttctagg
     1321 aaacattata gggacttatt gtgctcttat ggtaactcaa atactacata tgttagctta
     1381 ataaaaatat ggtataaaat taataattta aataagaaaa gtcatttcaa tttaagcaga
     1441 agcttttatt gaaatggctt tttttgtttg acaagataat ttatttcaag aagatatttt
     1501 gacatattta gtgtataata ttgtaaaatt tactaaaaac gagatatatt taatttttat
     1561 attgaatcga acgtctgtat gatgtataat aaatatacag aacgaatgtt tgcagaacgg
     1621 gtgttttttg aatttatttt aataatttat gaatatatat cagaatgcat aaatatattt
     1681 aaatataaag attaaaaaga atttaaagga gtgttgataa cttatgataa ttccatcaag
     1741 aagaatacaa gatataacat taacaaattt aaaaaatggt gaagtaactt taattgaatt
     1801 ggatgaaata tacaagaaga tgggattttt atttgttgtt agtgaaggca aattgaaaaa
     1861 gataaaaaag gagaatcatc actagaaatg aatgaaataa taaatattat aatagtttat
     1921 aaaacctaac ttatgtagaa tataaatact taagttaggt ttttttgtga taaagtttat
     1981 aagtactcaa ttttataaga taaataatgg tcttaagtga aaagtaaata agtttaaatt
     2041 ttaataagta ttatattgca taagaggtga tattattatg aaaatagtaa cctacaatat
     2101 tcataaaggt atggattcaa ataatagatt aactctaact aaaatggggt tatatttaaa
     2161 acaattggat tgtgatgtaa tttgtttgca agaagtatta tatcctcaat ttttagcttt
     2221 aaaagctgtt ttaaatatgg atggtgtctt tgctacaaat gtaaaaaaag taaatatgat
     2281 atatggtatt tgtacattta caacatttaa aatgttaaac agtaatcatt ttttcttaac
     2341 tagcaaaaaa gaacaacggg gagctctttg tataactata gatgcatatg gaagaattat
     2401 aaatgtaata aatactcact tgggacttga tagacaggag agagcaaaac aattagacga
     2461 aataatagac tataggaaca gattagtggg gatagttatt ttatgtggtg attttaatga
     2521 gaaaaatgtc tttttaagta tgtttaatga tatggctatt tcactaaaca agtcttactt
     2581 accaacattt gaaaaatcaa actctagaat agattacatt tttgttaata aaaatactga
     2641 actaaaagga tacacagtag aaaaaattta cctgtcagac cattatcctg taataggtta
     2701 catttaaatt tcaatataaa tgaaattagt tgttataaaa taaacaattt ttttgttaag
     2761 aaatagtaaa agaaatataa taaaaaatat caaaaataaa actaattata agacataaat
     2821 ttacaataaa gttacatttg attaaattcc ttgtttaaca aaagctattc aaatataatc
     2881 taaggtagtt ttgaaatggg aactctatta tgcaaaatta agctagtgtt aaaatgaggt
     2941 gaagttatgg gcagaagagc aacaagagtt tcaagattag gtaaagaaaa aaaacgaagt
     3001 gttttaattg tattggcagc tttagttata ataatgggtg gatttactta tttttttaac
     3061 agtaaatttc tgtacaatgg gaaaatcgct aaaaatgtat atatagaagg agtaaatgta
     3121 tctgatatga ctaaagcaga ggcactaaaa gcgataacag ataagtatac tccagaagac
     3181 ttaaatctta cttatgatgg gaaaaaatat acaatatcac caaaagatat tgatttaaaa
     3241 tatgatacag aagatgttgt aaaagatgct tatgaaagta caaaaaaagg gtcctatttt
     3301 caaaacttaa aaaaatatat tgatataaga gtgaataagg caaatatgaa gataaaagct
     3361 gaatatgatg aggcaaaact tagttccaag gtgtcttcta tagcagacag tataaatgtt
     3421 aagatgaaaa atgcttctat tagtgttgga agtggtggac taagttatac agactctatt
     3481 gtaggtagag aatttgattt ggcagcaaat aaagagtcta tatataatat gataaaaaat
     3541 aaagagcata aaactttaga acttaaagta aatcttcaaa aaccagatat aacaactgaa
     3601 caagtaaaaa ctgtaaattc tgttataggt caatattcaa ctacttattc acaggctgta
     3661 gagggtaggt catataatgt aggattatca gcaagaaaaa ctagtgatgt attattaatg
     3721 ccaggtgaag aattttcata caataaactt acaggaccta gtaataaagc aaatggttac
     3781 aaagatgcac ctgttatagt ttatggtaag ttagaacaat cagcaggagg aggtgtttgt
     3841 caaacatctt caacaattta taatgctgct cttctctcag gtatggagat aactcaagtt
     3901 acaaatcatt catcagcatc tacttatgta cctaaaggga gagatgctac tgtgagcgat
     3961 gggggattaa acttaaagtt taaaaatcca tataagcatc ctgtatatat taaaaattat
     4021 gcaggtggag gaagtgtatc atcagtaatt tatggtaatt ctggtgataa accaaatata
     4081 tctatagagg taaaccaaac tggtcagaat aaatattcta catatcgaat atttaaagat
     4141 tcaagtggaa aggttataaa aaaagaacat atatcaaata gttcttataa agaattaaaa
     4201 aaatagataa ttttttagag aaatatttgt agtataaaag ggagtgtctc aaaatgaact
     4261 ttttagttta tgagacactc tttttatgtg aaatcaaata tattttcatc atttcaacaa
     4321 tacaaaagaa gtgtatctct attttgagac agtctttttt gtaaaaatta acactagtag
     4381 ttcttgtttt aatattacaa tcattaatat atagaaaata ttcctatata ttggtataat
     4441 tgtaaataga tttagttata taaaatgtat gaggaggtga catggttgaa ttttataggc
     4501 aatagatatg aggtagtaaa ttctgatgac gttttagaaa ttaacaaaat ctataaagct
     4561 agagatgttt tttacaggaa aaatgtgtta ataaaagtta taaagcacaa taattatatc
     4621 tgtgaagatt ttgtttcaaa tttaattgat gaaagtactg ctttagatga gataaactct
     4681 ccttatatac taaaaataat agatgttggg attcactgta cagaagaaac aacattatat
     4741 tatgttgtaa gtgaagactt taatggtatc ggactagatg aattaattct aggtaattat
     4801 cttcatttag aagcaattgt aaacataatg atacaagttt taaaagcttt agaaatgata
     4861 cataggaatc attcatatca tggtagttta aaatcaagta gtataatagt agatacagaa
     4921 tacaatataa aaatttgtga ttttggaatt acaaaagcaa ataatggggt taacacaaga
     4981 agctatggaa acaggagata tttatgccca catcaactat gtataaatta tactgataaa
     5041 gaaagtgatt tttttgcaac aggtcttatt ctatttgaat ctatatttaa aaaattgcct
     5101 tttggagaat caaattcaga agaaaaaatg ctacaatcta ttgataaggg attagattgg
     5161 aatagtataa aagcaattaa tggaaataca gaacttataa atgttataaa aaggcttctt
     5221 ggaagaaata ataagtatac tagagccaat gatattatta ttgatttaag taaagtaatg
     5281 tatgaaaaag catatataga agttgaagat gagaaagaag aagaacagaa acaagacaaa
     5341 gttatacagg ttaaaaaaga atataaaagt aaagtgaaaa aaaaattaca caaaaaactt
     5401 gttgttgctg gtctagctat agtcatgatt tcaatgatta ttattagttc cataatataa
     5461 aaattgtata ttgagtattt taaaatcaat gtctataaat gatatacata gaaattttag
     5521 tgtagtctta aattatgaaa ggaagtggtt attatgttaa taaaaaataa tgaggtatta
     5581 aacgtctagg agtattttaa caaatagcca cctgctttta cagtttagaa taaaaaagct
     5641 gtggctaaag gtcacagctt tttgctactt tttaaaggca ggagcatatt aaaaaggagc
     5701 aaattatgag taatataaaa ataatagcaa gtaataaatg ttggattgaa aatatagcaa
     5761 ggaaacaact tgaagatatt tcaaagttaa atggaatttt aagagtagta ggattacctg
     5821 acctacatgc aggtaaaata cctgtaggtt tagcagttga gactaaaaat attatttatc
     5881 cacatataat tggaaatgat ataggatgcg gaatgacttt atttaaaact gggattctaa
     5941 aaaagaaatt taaaaaagac aaatggatta agttacttag taagattaaa gatttgtctg
     6001 atatagaaat aaaaaacaca tataaagaag aatgcccaat tttaaattta ggaactatag
     6061 ggagtggaaa tcattttgta gaaattcaat gtataagtga aatttataat aaagaacaat
     6121 ttgaacaatt aaaattttca tctgatgata ttatgatgtt agttcactgt gggtcaagaa
     6181 attatggaga agatatttta aaaaaatttt atgataaaga cggacttgaa gttgagagtg
     6241 agaaagcgat agattatatt aaaaaccatg ataatgcttt aatatgggca gagcgtaatc
     6301 gtgaagctat cagtaaaaaa attatgcagt caataggtac atctggagat gttgaaacaa
     6361 tattctctat aaatcataac tttatagaga aaagggaaga taaatttata catagaaaag
     6421 gtgctgtttc aagtgaaaga ggagctgtga ttataccagg ttcaagaggc agtctatcat
     6481 atatagttat gcctactgaa aatacagaga tatctttata ttctctatca catggagcag
     6541 gtagaaaatg gtctcgttca gtttgtaaat ctcgacttaa aagtaagtat agtaaagata
     6601 ctataaaaca aactaaattt aaaagtcaga ttatatgtaa tgatgtaaat ttattatttc
     6661 aagaagctcc acaggcatat aagaatatag agcaaattat tgaaagtttg atagaatatg
     6721 aacttattca agtagtggca actatgaagc cattgattac ttataaagga taatttttaa
     6781 agttaaaaaa gattaaaatt atattataaa tagctaattt tatggaacat ttaaaagtgt
     6841 attattatta gctatttttt tattttgtag agatgataat ttatagagct aaattctttg
     6901 atatataatg attacaaagt tagactctat tggaaactgc acatacatga attgatgaca
     6961 atataagtga ttccaaaata taaatgaaag tataagctta ttttcactat aaaaattttc
     7021 actatacaaa aacattaatg acattatttt tattgacata aatatataat ttatggaata
     7081 atattagaag cactagtata agtgtttaaa taagaataga aaaggagaag tgttttatga
     7141 aaaaaaatac taacattaat gagtccggag ttaaacttct taataaataa ggagtttgat
     7201 atataataac tccttattta ttaagttgtt cgcaaaataa ctattatcaa agtaaatgat
     7261 atagtgattt aaaagatgat gacttataaa taaaggagaa aaaataatga atagtaatac
     7321 atttgaaaga ttacaactaa atgaagttaa agaattaata caaatacatt gtgtaagttc
     7381 gctaggaaaa aatttaatag ataaattaac tcctagtggg aatattggag tagtaagaag
     7441 gaaattagca gaaaataaag aagctagaaa gataattgaa aatagtaatc acataccact
     7501 tgaaggattg tttaatgcag gttctacaat agataaaatt gaaaaaggaa tgataataga
     7561 gcctattgaa cttgtaaata ttgaagattt tttaagaggt tgtaggaaga tgaaagcttt
     7621 tatgttagaa aaagaatttt attcacctac attaagttct tatgctttaa atataacaga
     7681 atgtaaaagt atagaagatg agataaatta ctgtataaaa tctaataaag ttgattctaa
     7741 tgctagtaaa gaattaaaaa aagtaagacg aaatatagaa attacagaag gaaagataaa
     7801 agatagatta aataaattta taacatcaac tgtaaataaa aaatatatac aagagtttat
     7861 aataagtaaa agaaatgata gatatgtaat accaattaaa tcttcttata aaaatgaagt
     7921 taatggtact atattagata catcatcaaa aggaaatact gtatttattg agcctattag
     7981 tgtttcaaat ttaagtacag agttaactat gctaaaagct gatgaaacta ttgaagaata
     8041 caaaatatta tcttatttaa cagaacttat atttgagaag ataagtcaga taaaattaaa
     8101 tatagaaata ctttcagaat acgatatggt atttgcaaaa gctaagtata gtcaaaaaat
     8161 aaaaggaatt actccaaaaa taaataataa tggctatata aaaataatta aaggtaaaca
     8221 tccacttcta acaggagatg cagtaccttt agattttgaa ataggcaaaa attatagaag
     8281 tcttataata acaggtccta atgcaggagg aaaaactgta acccttaaga ctgttggttt
     8341 actaacttta atggtacaat gtggatttga tatttctgca aaagaaggta gtgaatttag
     8401 tgtatttgaa aaagtttttg tagatatagg agataatcaa agtattgaaa atgccttaag
     8461 tactttttca tcacatataa aaaatattgc agagattatg agtttatcaa ataattctac
     8521 tcttgttctg tttgatgaaa taggaagtgg aactgaacct aatgaaggag ctggtttagc
     8581 aatatcatta ttagaagagt tttacaaaat gggatgtata actatagcat caactcatta
     8641 tggagagata aaaaagtttg ctacattgca tccagaattt gaaaatgctg gtatgatgtt
     8701 tgataaagaa acattagaac cattgtataa gttaactatt ggaaaatctg aagatagtaa
     8761 tgctcttttt atttctaaaa aaatgggaat aaaaaataga gttttagata gggctaaaga
     8821 atatgtaatt gatagaaact acaatttaga tttagttaaa agaagtaagc tacaatctaa
     8881 tattgaagat aaaaatagct ctgaagtcat tttaaactac acagattata atgtagggga
     8941 taaggtaaaa ttactagatg aagatgattt tggaattgta tataagccta tggataagtt
     9001 taataatgta gaagtattgt ttaatgataa ttttatttca gtaaatataa aaagattaca
     9061 acttagcata aaagcatcag aattgtatcc ggaagggtat gatttagatg tattatttac
     9121 tagttttaaa gaaagaaaac tagaaaaaga tattcaaaga ggttcaaaaa aaactcttaa
     9181 aaagattcag aaagaaatta aagagaatag aaaataaata taaaaagcac aaaagaaagc
     9241 aatctaaaga gaaaatttta tctttagatt gctttcttat taacatattt tcataaatcc
     9301 aattactaac aagattaaac cacttaacca agataagttt aaatttatgt ttttaacaaa
     9361 catatttcct agtaaacaac caaataatac tgcaattata tttgatatta aagaaaatag
     9421 aataacttgt atataattca cacctacaag accactacca aaaccgatag ctaatccatc
     9481 taaacttaaa gcaagtgcaa gtgtaaatgc ttcagcagat ttaagcactt tagatttact
     9541 tgtatctaag acatcaggtt cgatacttac attaaaattt aatttaaggt ctaacatatt
     9601 aaaattataa ttctttgagt gtatagattt tttcttaaaa taagatttaa aaaaactttc
     9661 aattagtcta gcacttccta ataaaaataa gataaagaaa catattaaca ttgttatatt
     9721 ttgaggtaaa aagtctctaa ctattgtacc tgcaaaaagc gatattgcta aaatagctga
     9781 acaaactacg ctgataatta tatttgaata taagggaact ttaactttgt ttacgccata
     9841 tccaaagctt gtcacaaagg catctataca aagagaagta actaataaaa ttgattcaat
     9901 cacagtagat taacatcctt tctcttaaat tttgtactta tattagttat agtattgtaa
     9961 aaatggataa attgttacag ataatccaat agattgttga taaaatttca acatcataca
    10021 acacctaaat aacaaaaaaa tcatattata tagtaatata aaaccaatca taataaagca
    10081 ggtgaaaata atggaacaac atagcaaggt tgggaatttc ggtgatttgt tgaagatttt
    10141 attagaagaa aaagaattat caatgggaga actaagtaaa aaaagtggaa ttgataaatc
    10201 tactatatct cgtatagcta ataataaaca aaagccaaat ataaatcatt tagaaaagat
    10261 ggcaatacat ttaaacatta acctagaaga actattgaaa gcttcaggat atgaatttag
    10321 aaacagcaat aatcaaatat ttaatacaga tagtgatttt agtaactttg atgatattct
    10381 tgggtttgca aatttaatca atgataagaa ctttaatgga aatattgaaa aagagttaag
    10441 taagtgtaag ttatatgtcc aaacagatga aggtaaaaaa ttactttttg ataattttaa
    10501 taaaaaaata gattctattg aaaaacaagg tcaatttaca gatagattaa gacaaatgta
    10561 tgccgatttt tgtacagatg gacttacaat aaagaaatat ttattgattg gaagtatgct
    10621 actttacttt gttatatcaa cagatgttat tccagacttc gtgttcccaa taggatttat
    10681 ggatgattta gtagcattaa atatagttac aaaattactt aaaaatgata agta
//
LOCUS       DAEPXF010000134        10678 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain HB27
            SAMN13376389-rid12479423.denovo.141, whole genome shotgun sequence.
ACCESSION   DAEPXF010000134 DAEPXF010000000
VERSION     DAEPXF010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13376389
            Sequence Read Archive: SRR10518432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10678)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10678)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001069624.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001069624.1
            Genome Coverage       :: 315x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/15/2021 01:54:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,165
            CDSs (total)                      :: 4,076
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 89
            rRNAs                             :: 10, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 10 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 19 of 69
            Pseudo Genes (incomplete)         :: 44 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 10 of 69
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10678
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13376389-rid12479423.denovo.141"
                     /strain="HB27"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="China: Hebei"
                     /collection_date="2017"
     gene            115..1815
                     /locus_tag="KRL67_002838"
     CDS             115..1815
                     /locus_tag="KRL67_002838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH1523704.1"
                     /translation="MKRYLKTYGVALIIVIIYAFIKLPVLRLDFTSLFSVGIIFFVVA
                     GILDMMLDRNERASKMAKYNFSIAIALLVYIVVVPFITSTPVLHAKSYRELLGKVTES
                     KFTDDISPVSVNDIRLVDEDMAMKLGDKKLGEVPAIGSVSKLGKFHIQNVDGELYWVA
                     PLVHRDIIKWITSLSGTSGYVKVSASNPQDVQLVQEIDGKPIKIVYQPEAYLHQDLQR
                     HLYIHGIVNVGMTDFTLEINDEGRPYWVVSLYEHKIGYGGANATGIATVDAETGKINV
                     YDVKNTPKWVDRIQPQSFVTDQIKDWGVYVNGFLNSVISEKGVLVPTEGTSLVYGNDN
                     RSYWYTGITSSGGDESTIGFMLVDSRTKEARLYKQPGATETAAMKSAEGNVQEKNYEA
                     TFPVMYNILGQPTYVSSLKDKAGLVKRVAFVSVEDYNVLGIGEDKNEALRNYKDALES
                     KGNDLKLDNDLKDEVVEGTVTRISPDVRGGNTNYYVTLDSNKDIIFRATSKVSSELPL
                     TQVGDKVKISYSKEESGVIEMSEFDNLNIANFEDKKAKEGTGTDENTDSKDSENQGNV
                     "
     gene            1985..2602
                     /locus_tag="KRL67_002839"
     CDS             1985..2602
                     /locus_tag="KRL67_002839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH1523705.1"
                     /translation="MTNMEKEILLLISRVRCVTESQFRKIYGDQKRYNKKNFKKTLRK
                     MCSEYTLKKYPCDVDYYNYKDLSYVYYLNGSKLFKGDDLIKALVGSELVVRINTAGCD
                     VKRFYRNVSVDDVKYDLFIEYTDQFGNIKQVLVDVDLNNEIDISKYENLSKKIDKSTI
                     PFFKVPNVLVVTPDNISELNDLERYDNEYSINFIDLGLNKLFNCI"
     gene            2963..4156
                     /locus_tag="KRL67_002840"
     CDS             2963..4156
                     /locus_tag="KRL67_002840"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HBH1523706.1"
                     /translation="MSIIVQKYGGSSVADTEKIKSIAENIIERRKENPQMVIVVSAMG
                     KSTDEYITLAKELSNEPSKRELDALMSTGEMISASLLSIALNALGCKAISYNAYQLNI
                     KTSGLHGKSQIDDINVNRIKNSLDEGNVVIVTGFQGINEDGDVTTLGRGGSDTSAVAL
                     AVKLNGKCEIYTDVDGIYFTDPRKYSKASKLDEIEYEEMLELASLGAQVMHSRSIELA
                     QKYGVEIYVGRTCGTEKGTYIRGGKDMKLEDKVITGLATSDDDSSITIKDFKAENISS
                     LFEDIATIGISVDMISQTAPILDKISVSFTVPKEELGECKKIVSKYTDEEHVVIDNNI
                     TKFSLVGLGMKNTSGVAAKVFKIFNENGIMIKLITTSEIRITCAINSDDKQVAIEKIA
                     EVFNI"
     gene            4174..5466
                     /gene="lysA"
                     /locus_tag="KRL67_002841"
     CDS             4174..5466
                     /gene="lysA"
                     /locus_tag="KRL67_002841"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HBH1523707.1"
                     /translation="MKLHGTMTVNNDELYIGGVSCLELSKKYQTPLYVFDEALVRQNC
                     REYIEYFKAREGKNKVAYAGKAFLPLYMCNLINEEQMCLDVVSGGELYTAYKSNFPME
                     KVFFHGNNKTKEEINMGLDLGVGKFVVDNFYELDLLEALCEDKGKTQEIYFRITPGIE
                     AHTHDYIKTGQIDSKFGFALVNGDLFRAIEKLSEYKNIKLVGLHAHIGSQIFDVEPYI
                     DTVDIMMNLVKEIKEKFNIQLTEIDLGGGVGVYYSEGDKPKTIKEFCETIITKAETSC
                     KELGIEVPTLVIEPGRSIVANAGSTLYTVGSIKDIKDVRVYVSVDGGMTDNVRPSLYQ
                     ASYECSIVNKINHEAMNHVTIAGKCCESGDILIGDINIMDIKSGDILITTSTGAYGYS
                     MSSNYNKIPRAAVVSVFDGNDKIICKRQSYEDLLALEV"
     gene            5683..6342
                     /locus_tag="KRL67_002842"
     CDS             5683..6342
                     /locus_tag="KRL67_002842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH1523708.1"
                     /translation="MRGRVILLISILSIFLVGCSFNKKDEINLVEQGKTYLEKHNYKK
                     AMESLSSALEEDNTNENARAMYMQAMRMSNMTEFEELKNYEGAIKELKLIEKIKNGSS
                     VIKEEATKKKEELTKLEKEQNEAEEQRKKKAKDTAGEDRYRVESDARKSSYSGSSKNN
                     KSSSKNNDKKSDSNKSDNNNSSSQNQEKPNNSNPTPAPTPQDTSGGSDSGGNSPNSNN
                     Q"
     gene            complement(6498..7583)
                     /locus_tag="KRL67_002843"
     CDS             complement(6498..7583)
                     /locus_tag="KRL67_002843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HBH1523709.1"
                     /translation="MIIISKENIKYYLIVVFIGILFFKFINTPSDFISSIEGFLKFFS
                     PFLLAILLALLLNPLVMLFEVKFKAHRLLAIFLSYIFIGFILAFAIRLLIPSIANTLN
                     RLINEMPMYTDYIDSFIEKNMSNIDFLKTLIPHIQHSLDNLLKEASKFIGKIPKNFLI
                     YTLSISSMLFNMTMGFILSIYIIYDKEKIALGFKRFLYSSTARNKADNIIEFFRTTHD
                     IFYDYLLGRILDSLIIGIIAFLGFQFVIRIENALFLASIIFLGNIIPYFGPFIGAIPP
                     IAMTILYSPQKTIWVIVFLFILQQLDGNFIEPKVMGNQVGIGAIWVISAILVGQSLFG
                     FIGVFLSVPIAAVVKTYVDKYIDNRLQ"
     gene            7774..8490
                     /locus_tag="KRL67_002844"
     CDS             7774..8490
                     /locus_tag="KRL67_002844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HBH1523710.1"
                     /translation="MRINKYIASCGIASRRKAEEIILGGRIKVNGSVVKELSFNIDEE
                     KDIVEFDNKKVKPSENYIYIVLNKPEGYITTVKDQFNRPSVIDILKDVRERVYPIGRL
                     DYETSGLLILTNDGDLTYKLTHPKHEIDKTYVASVKGIISDDEIRKFETGLKIEDYTT
                     APAKIRVTKENKEKNYSVCEITIHEGRNRQVRKMCKAINHPVLNLRRISVGKIVLKDT
                     KVGEYRYLTEDEIKYLKSIK"
     gene            8548..9126
                     /locus_tag="KRL67_002845"
     CDS             8548..9126
                     /locus_tag="KRL67_002845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HBH1523711.1"
                     /translation="MAYAKYLTKITDINKFYLENIINEGDIVIDATMGNGYDTRYLAE
                     KVGEKGFVYAFDIQEEAIKSTRKKLEKEGYIDRVKLIFDGHEKMNQYIKEEVSCVLFN
                     LGYLPRAKHSIITKPDTTLKAIKLSLEMLKENGVISIAIYTGHDGGQEEKDCIYNLVN
                     NLNQDEFNVLESKFLNQVNNPPQLILIEKKKA"
     gene            9520..10392
                     /locus_tag="KRL67_002846"
     CDS             9520..10392
                     /locus_tag="KRL67_002846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HBH1523712.1"
                     /translation="MEETNEMKDSKHLISNIQSQYTRLSKGQKLIAQYILNNYDKVAF
                     MTACKLGETVGVSESTVVRFANALGYSGYPKLQAALQELIKNKLTTVQRVEMAHDYSD
                     DFAILNKVLKSDIDNIRSTLEEIDERAFKEASNKLLRARKIYILGMRSSFVVAQYLGF
                     YLDIILDNVHIIRMDMGDAFEQIVRINEEDVIVAISFPRYSKKSYQIVNYAKEKGAHV
                     ISLTDSLFAPVASLADNTLLVKSNMASFVDSLVPALSISNALAISVGMKEKEDIKQHF
                     DDLEQIWKRYSVYE"
BASE COUNT         4406 a         1113 c         1854 g         3305 t
ORIGIN      
        1 ataactataa atatataaag atatcttaat atataataaa tatgataaat atttattttt
       61 tgtaaatata tggatataat agtaataata acatttttta gagaggagta ttaaatgaaa
      121 cgatatctta aaacatatgg ggttgcatta ataattgtaa taatttatgc atttattaaa
      181 ttacctgtac taagattaga ttttacatct ttattttcag taggaataat tttctttgtt
      241 gtagcaggaa tactagatat gatgttagat agaaatgaaa gagcatctaa aatggctaaa
      301 tataattttt caatagcaat agctttgctt gtgtatattg tagtagttcc ctttataact
      361 tctacacctg tacttcatgc aaaatcatat agagaactat tgggaaaagt aaccgaaagt
      421 aaatttactg atgacattag ccccgtcagt gtaaatgaca ttcgtcttgt agatgaagat
      481 atggcaatga aattgggtga caaaaagctt ggagaagttc ctgcaatagg gagtgtatcc
      541 aaattaggaa aatttcatat acaaaatgta gatggagagt tgtattgggt agcacctctt
      601 gttcacagag acataattaa atggataaca tctctaagtg gtacaagtgg ttatgtaaaa
      661 gtatcagcta gtaatcctca agacgtccaa ttagttcaag aaatagatgg aaagccaata
      721 aagatagttt accaaccaga agcctattta catcaagatt tacaaagaca tttatatata
      781 catggtattg tgaatgttgg tatgacagat ttcactcttg aaataaatga tgaaggaaga
      841 ccttattggg ttgtaagttt atatgaacat aaaattggct atggaggagc aaatgccaca
      901 ggaatagcta ctgttgatgc agaaactgga aaaataaatg tttatgatgt taagaacaca
      961 cctaaatggg tagatagaat acaaccacaa agctttgtaa cagaccagat taaagactgg
     1021 ggtgtttatg ttaatggatt tttaaattct gttatatcag aaaagggagt tttagtacca
     1081 acagaaggta catcattagt ttatggaaat gataataggt cttattggta tacaggtata
     1141 acatcttcag gtggagatga atctacaatt ggatttatgt tagtcgattc tagaacaaaa
     1201 gaggctagat tatataaaca accaggagct actgaaacgg ctgcaatgaa atcggctgaa
     1261 ggtaatgttc aagaaaaaaa ttatgaagct accttccctg taatgtataa tatattgggt
     1321 cagccaacat atgtatcatc attaaaagat aaggctggtc ttgttaagag agttgcattt
     1381 gtatctgttg aagattataa tgtacttgga ataggtgaag ataagaatga agcattaaga
     1441 aactataagg atgcattaga atctaagggt aatgatttaa aacttgataa tgatttaaaa
     1501 gatgaggtag tagaaggcac agtgacaaga ataagtccag atgtgcgagg tggtaacact
     1561 aattattatg taactttaga ttcaaataaa gatataatat ttagggctac ttcaaaagta
     1621 tctagcgaat tgccattgac tcaggttggt gataaagtca aaatatcgta ttcaaaagaa
     1681 gaatctggtg taatagaaat gtcagaattt gataacttaa atattgcaaa ctttgaagat
     1741 aaaaaagcaa aagaaggaac aggaactgat gaaaatacag attccaaaga ttctgaaaat
     1801 caaggtaatg tataatttaa ataaaaagct tgataacagt atttagcata gtgatagcta
     1861 atttttatag aatttaattc tataaaaatt agctatttta ttttaattct ttgtaaattt
     1921 aatgtcataa aattaatata atattagtac aactttgcaa ataatacttc aaggagaatg
     1981 tactatgacg aatatggaaa aagagatatt acttttaatt agcagggtta gatgtgttac
     2041 agagtctcaa tttagaaaaa tttatggaga ccaaaaaaga tacaataaaa aaaatttcaa
     2101 aaaaactttg agaaaaatgt gtagtgaata tacactaaaa aaatatcctt gtgatgtgga
     2161 ttattataat tataaagacc tctcttatgt atactattta aacggtagta aactttttaa
     2221 gggagatgat ttaataaagg ctctagtagg atcagaatta gttgttagaa tcaatactgc
     2281 aggttgtgat gtaaaaagat tttaccgaaa tgttagtgta gatgatgtga aatacgattt
     2341 atttattgaa tatacagacc aatttggtaa tataaagcaa gttttagttg atgttgactt
     2401 aaataatgaa atagacattt ctaaatacga aaatctttct aaaaaaatag ataaatctac
     2461 aattccattt tttaaagtac caaacgtttt ggtagttaca ccagataata tatctgaatt
     2521 aaatgattta gagagatatg ataatgagta tagcataaat tttatagatt taggattaaa
     2581 taaactattt aattgtatat aagctaactt tagaaaagaa aactatcggt ttgccacatt
     2641 ttgtaaaata attgttgcaa tattatatag aatatgataa aactatatta ataaattaat
     2701 taaaaataat gagcctaggg tagaggtagc tgtattcaat agtattcaat aggagctggt
     2761 aagcgttgat aattgatgaa agggtatata gccgaagaaa ttttgttgac ggacaaattt
     2821 tctggactta tgcagaatat gcataaggct gtcactttta aagtggtgag ctacaaggtt
     2881 acttggtcta tgaaatttac acgtccaaag tatacctttg ggcgttttta tttatcaaaa
     2941 tttagcttga gggggagaaa gcatgagtat aattgtccaa aaatatggtg gaagttcagt
     3001 agcagatact gaaaaaataa aatcaatagc agaaaatata atagaacgaa gaaaagaaaa
     3061 tccacaaatg gtaatagttg tgtctgccat gggaaaatcc actgatgaat atataacttt
     3121 ggcaaaggaa ttgagtaatg aacctagtaa gagagaatta gatgctttaa tgtctactgg
     3181 tgaaatgatt tcagcatctt tattatcaat agcattaaat gcacttggat gtaaagctat
     3241 aagttataat gcttatcagt taaatataaa aacgagtggt cttcatggaa agtctcaaat
     3301 tgatgatata aacgtaaaca gaattaaaaa tagtttagat gaaggaaatg tcgttatagt
     3361 aactggattt cagggaatca acgaagatgg tgatgtaacc actcttggaa gaggtggctc
     3421 agatacatct gctgtagctc ttgcggttaa attaaatgga aaatgtgaga tatatacaga
     3481 tgttgatgga atttatttta cagacccaag aaaatattca aaggctagta aacttgatga
     3541 aattgaatat gaagaaatgc ttgagttagc gagtttagga gcacaagtaa tgcattcaag
     3601 aagcatagag cttgcacaaa aatatggtgt tgaaatctat gtcggacgta catgtggtac
     3661 agaaaaagga acatatataa gagggggaaa agacatgaaa ttagaggata aagtaataac
     3721 aggattagct actagtgatg atgacagttc tataacaata aaagatttta aagctgaaaa
     3781 tatatctagc ttatttgaag atatagcaac tataggtata agtgtagata tgataagcca
     3841 aactgcacct attttagata agataagtgt atcatttaca gttccaaaag aagagttggg
     3901 agaatgtaaa aagatagtat ctaaatatac agatgaagaa catgtagtga ttgataataa
     3961 tatcactaaa ttttctttag taggtcttgg tatgaaaaat acttctggag ttgctgctaa
     4021 agtatttaag atattcaatg aaaatggtat aatgataaaa cttataacta catcagaaat
     4081 tcgtataact tgtgctataa attctgatga taagcaagta gctatagaaa aaatagcaga
     4141 agtatttaat atataaaatt tggggaggct attatgaaac ttcatggaac tatgactgta
     4201 aataatgatg aattatatat tggaggagtg agttgtttag aacttagtaa aaaatatcaa
     4261 actccacttt atgtatttga tgaagcttta gttagacaaa attgtaggga atatatagaa
     4321 tattttaagg ctagagaggg aaaaaataag gttgcttatg cagggaaagc ttttttacct
     4381 ctttacatgt gtaatcttat aaatgaagaa caaatgtgtt tagatgtagt ttcaggaggc
     4441 gagttatata cagcttataa atcaaatttt cctatggaaa aagttttctt tcatggaaac
     4501 aataagacta aagaagaaat aaacatggga ttagatttag gtgtaggtaa atttgtagtg
     4561 gataattttt atgaattaga tttgcttgaa gcattatgtg aagataaagg taaaactcaa
     4621 gagatatact ttagaataac tcctggtata gaagctcata cacatgatta tataaaaact
     4681 ggacagatag attctaaatt tggatttgct ttagtaaatg gagatttatt tagagcaata
     4741 gaaaaattaa gtgaatataa aaatattaaa cttgtgggtc ttcatgctca tataggttca
     4801 caaatatttg atgtagaacc atacatagat acagtggata taatgatgaa cttggtaaaa
     4861 gaaataaaag aaaaattcaa catacaacta acagaaattg acctaggtgg aggagttgga
     4921 gtttattata gcgaaggaga taaacctaaa actataaaag agttttgtga aactataatc
     4981 actaaagcag aaacttcatg taaagagctt ggaatagaag tacctacgtt ggtaatagaa
     5041 ccaggaaggt caatagttgc aaatgcagga agtacattgt atacagtagg ctctataaaa
     5101 gatataaaag atgttagggt ttatgtaagt gtagatggag gtatgacaga taatgtaaga
     5161 ccttcgttgt accaagctag ttatgagtgt agtattgtga ataaaattaa tcatgaggct
     5221 atgaatcatg taacaatagc tggaaaatgt tgtgagagtg gagatatatt aattggagat
     5281 ataaacataa tggatattaa aagtggagat atacttataa caacatcaac tggtgcatat
     5341 ggatattcaa tgtcttcaaa ttataataaa ataccaagag cagcagtagt atcagtattt
     5401 gatggaaatg ataagataat ttgtaagaga caaagctatg aggacttatt agctttagag
     5461 gtataaaatg gagaagtata taataaaaat taaatattaa agtttattat gagcagattt
     5521 gctaggatat gtatctctaa attaattttt ggtttattgt atcgttcgta gcatatctgc
     5581 ttattttttg ttttactaac aaatttcttg taataaatat aaaatatttt aaaaacttta
     5641 taattatgat aaaatttaaa gtatatgaca aggagggatt taatgagagg aagagtaatt
     5701 ttattaatat cgatattatc tatatttctt gtaggttgtt catttaataa aaaagatgaa
     5761 ataaatttag tagagcaagg aaaaacatat ttagaaaaac ataactataa aaaagctatg
     5821 gaatcattat cctctgcact ggaagaagat aatactaatg aaaatgcaag agctatgtat
     5881 atgcaagcta tgagaatgtc aaatatgacg gagtttgaag aattaaaaaa ttatgagggt
     5941 gcaatcaagg aacttaaact tatagagaaa atcaaaaatg gttcctctgt tattaaagaa
     6001 gaggcaacta aaaagaaaga agagttaact aaattagaaa aagaacaaaa cgaagcagag
     6061 gaacaaagaa agaaaaaggc aaaagatacg gctggagaag ataggtatag agttgaatca
     6121 gatgcaagga aatcttctta tagtggaagt agtaaaaata ataaatctag ttctaaaaat
     6181 aatgataaaa agtcagatag taataagtct gacaataata acagttcttc tcaaaatcaa
     6241 gaaaagccaa ataacagtaa tccaacacca gcaccaactc cacaagatac tagtggtggt
     6301 tcagatagtg gtggaaattc tccaaactca aataatcaat aagtataaaa tatatacata
     6361 aatttaatga acaaaatata tattataaaa aatatctctt agcaattttt tcttactaag
     6421 agatattttt ttgagaaata atttaataaa taacagttaa attttaaact aaaaattaaa
     6481 gtcacagtag agataagcta ttgtaaacga ttatctatat atttatcaac ataagttttt
     6541 acaacagcag ctattggtac tgaaagaaaa actcctataa aaccaaatag agattgacct
     6601 accaaaatag cagatattac ccatatagct cctataccaa cttgattacc cataactttt
     6661 ggttctataa aatttccatc taattgttgc aatataaata gaaatacaat gacccatata
     6721 gttttttgtg gactatataa tatagtcatg gctattggag ggattgcacc aataaaagga
     6781 ccaaaatagg gaattatatt acctaagaat attattgaag ctaaaaataa agcattttct
     6841 attctgataa caaattgaaa tcctaaaaat gcgattatac caataataag agagtctaat
     6901 attctaccaa gcaaataatc atagaaaata tcatgagtag tcctaaaaaa ttctataatg
     6961 ttatctgcct tgtttctagc agtagaagaa tatagaaatc tcttaaatcc caatgcaatt
     7021 ttttctttgt catatataat gtatatagat agtataaatc ccattgtcat attaaatagc
     7081 atagaagata tactaagtgt atagattaag aagtttttag gaattttacc aataaattta
     7141 cttgcttctt ttaataaatt atctaaacta tgttgtatat gaggaattaa agtttttaga
     7201 aaatctatgt ttgacatatt tttttctata aaactatcta tataatcagt atacataggc
     7261 atttcattta tcaatctatt taatgtatta gctattgatg gaattaataa cctaatggca
     7321 aaggctaaaa taaaacctat aaatatataa gataaaaaaa tggctaaaag tctatgtgct
     7381 ttgaacttta cctcaaaaag catcacaagt ggatttaaaa gtaaagcaag taaaatagct
     7441 aataaaaatg ggctaaaaaa ctttaagaat ccttcaatac tagatataaa gtctgatgga
     7501 gtattaataa atttaaaaaa taaaattcca ataaacacga ctattaaata atattttata
     7561 ttttcctttg aaataataat cacttcctta aaatagtgtt tagtcagcta ttacatatta
     7621 atagtatgtg aaaagaaatt acaattattt aggaaaatta aataattttt ttgcttgttt
     7681 ttaattaagg tctagtatag tataataatg tagtaaaatt aacattaata gaaatattaa
     7741 acaacacata attaagataa ggaaggatta agtatgagaa ttaataagta tatagcttct
     7801 tgtggaatag cgtccagaag aaaagcagaa gaaataattc ttggaggaag aataaaggta
     7861 aatggaagtg tagtaaaaga gctatctttt aatattgatg aagaaaaaga tatagtagaa
     7921 tttgataata aaaaagtaaa accaagtgaa aattatatat atatagtatt aaataaacca
     7981 gagggatata taactacagt aaaagaccaa ttcaatagac ccagtgtaat agatatactt
     8041 aaagatgtaa gggaaagagt atatccaata gggagattag attatgaaac tagtggatta
     8101 ttaatactta ctaatgatgg tgatttaaca tataagctta cccatccaaa gcatgaaatt
     8161 gataaaacgt atgtggcaag tgtgaaggga attataagtg atgatgagat aagaaaattc
     8221 gaaacaggac taaaaataga agattacaca acagcacctg ccaagataag ggtaactaaa
     8281 gaaaataaag aaaaaaatta ttcagtgtgt gaaataacta tacatgaggg tagaaataga
     8341 caagtaagaa aaatgtgtaa agctataaat catccagttt taaatttaag aaggatttct
     8401 gtagggaaaa tagtattaaa agacaccaaa gttggagaat atagatacct aacagaagat
     8461 gaaataaaat atttaaaatc aataaaataa gataaaaata aatggagaaa aaatgaacta
     8521 aagttaatag tagggagaaa tagtattatg gcatatgcaa agtatttaac taaaattaca
     8581 gatataaata aattttattt agaaaatatt ataaatgaag gtgacatagt aattgatgct
     8641 actatgggaa atgggtatga tacaaggtat ctagctgaga aggttggaga aaagggcttt
     8701 gtatatgctt ttgatataca agaagaagca ataaagtcta caagaaagaa actagagaaa
     8761 gaaggatata tagatagagt aaaattgata tttgatgggc atgaaaaaat gaatcaatat
     8821 ataaaagaag aagtttcatg tgttttattt aatttgggtt atcttccaag agcgaaacat
     8881 tcaattataa ccaaaccaga tacaacttta aaggctataa agttaagctt agaaatgtta
     8941 aaagaaaatg gagtaataag cattgccata tatacagggc atgatggagg tcaagaagaa
     9001 aaagattgca tatataacct tgtcaataat ttaaatcaag atgaatttaa tgttttagaa
     9061 tctaagtttt taaatcaagt caataaccca ccacaattga ttttaattga aaagaaaaaa
     9121 gcatagtaaa tactaaaaaa cttgacaaga ttctattaag agttaatata tatagtgtga
     9181 cctattaaaa atactttaaa taataagtat acaacaactt atcctcaaaa caaaatatac
     9241 atctaaatat gcaaaataag atagataact tttaaatgag taaaaattga aaaatttaaa
     9301 agaaatattg gataaatatt aaaaaatgca aggaaagatg gtataataaa tttaatcgaa
     9361 aatactatgt gaagtataag gtacagaaaa gttagaattt tatgagttta agaagctatg
     9421 ataaaaacta agatgaaatt gagataaaaa ataaaatttt tagagaaaaa gacagtgtta
     9481 ataaaacaaa atagtttata taactaaaag gagaaaaaaa tggaagaaac aaatgaaatg
     9541 aaggacagta aacatttgat atcaaatata caatctcaat atacgagatt gagtaaaggt
     9601 caaaaactta tagcacaata cattttaaat aattatgata aagttgcatt tatgactgca
     9661 tgtaaattgg gtgaaactgt aggagtaagt gagtctacag ttgtaagatt cgcaaatgca
     9721 ttaggttata gtggatatcc taaattacaa gctgcacttc aagaactcat aaaaaataaa
     9781 ctaactacag tacaaagagt tgaaatggca catgattatt ccgatgattt tgcaatacta
     9841 aacaaagttt taaaaagtga tatagataat ataaggtcta cactagaaga aattgacgag
     9901 agagcgttta aagaagcttc taataaactt ttaagagcga gaaaaatata tatacttgga
     9961 atgaggagtt cttttgtagt agctcaatat ttaggttttt acttagatat aatacttgac
    10021 aatgttcata taattagaat ggatatggga gatgcttttg agcagattgt aagaataaat
    10081 gaagaagatg taatagttgc tattagtttc cctcgttatt ctaagaaatc ttatcaaatt
    10141 gtcaattatg ctaaggaaaa aggagcccat gtaatatcat taacagatag cttatttgcg
    10201 ccagtagcat cacttgcaga taacactctt ctagttaaga gtaatatggc ttcatttgta
    10261 gattctctag ttccagctct tagtatttca aatgcccttg ctatatctgt tgggatgaag
    10321 gaaaaagaag atattaaaca gcattttgat gatttagagc aaatttggaa aagatattct
    10381 gtgtatgagt aaagtttgta tattaataag gctatggaat tgagaatcat agccttatta
    10441 gtaaattctt gattatcact taaaaaataa attttcgttt aaataataca aataaatatt
    10501 tcgagactca aaacataaag atgtattctg aatacatctt tactacgtca actgatttat
    10561 taaagaaagc atttcccttc atggagagtg ctttttttat ttctctaaac aatataattt
    10621 ccttaattaa caattataaa attaatttaa tttgtattta agtatataat atcttatt
//
LOCUS       DAEPXF010000135        10609 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain HB27
            SAMN13376389-rid12479423.denovo.142, whole genome shotgun sequence.
ACCESSION   DAEPXF010000135 DAEPXF010000000
VERSION     DAEPXF010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13376389
            Sequence Read Archive: SRR10518432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10609)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10609)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001069624.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001069624.1
            Genome Coverage       :: 315x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/15/2021 01:54:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,165
            CDSs (total)                      :: 4,076
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 89
            rRNAs                             :: 10, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 10 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 19 of 69
            Pseudo Genes (incomplete)         :: 44 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 10 of 69
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10609
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13376389-rid12479423.denovo.142"
                     /strain="HB27"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="China: Hebei"
                     /collection_date="2017"
     gene            <1..324
                     /locus_tag="KRL67_002847"
     CDS             <1..324
                     /locus_tag="KRL67_002847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HBH1523713.1"
                     /translation="AVEKADCYTGNTIESERFFPGWLYDEEDEFVQAAYKGLKEAGID
                     SEITQYSFCTNGSHYAGEAGIKTIGFGPSKENLAHTIDEYIEQEQLFIGTEGYYGILK
                     SVYGK"
     gene            457..1827
                     /gene="hydA"
                     /locus_tag="KRL67_002848"
     CDS             457..1827
                     /gene="hydA"
                     /locus_tag="KRL67_002848"
                     /EC_number="3.5.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropyrimidinase"
                     /protein_id="HBH1523714.1"
                     /translation="MGTILRGGTIITSDKTYISDLRIEDEKIVEIGNNLEINGDKVID
                     ATGKIVIPGGIDTHTHFDMNAGSITTADNFETGTRAAIAGGTTTILDFAEANEGENLL
                     QGVEAYHKKASGNCYCDYGFHMTITCLNKDTFNHMEKLISDGIVSFKMYMAYDGMKVD
                     DGAIYRVLKKARDLGCIVEFHCENGDLLDVLIEENIQNGNLEPKYHPLTRPNIVEKES
                     VSRLADITKLSNSKSYVVHLSCKESLETVKTAREKNIDMIVETCPQYLLLEDNLYNKD
                     KFEGAKYVMSPPLRKKEDINYLWEGLASGDIQTVGTDHCSFNFKGQKDLGIDDFSKIP
                     NGAPGVEHRLALLYTYGVLENRISANKFVEVTSTNAAKIFGMYPKKGEIAVGSDADIV
                     ILNVNKEETISYKTQKQNVDYTPYEGFKIKCKVEDVFLRGNHVVQSCNVKEHPTGQYI
                     KRKITK"
     gene            1845..3251
                     /locus_tag="KRL67_002849"
     CDS             1845..3251
                     /locus_tag="KRL67_002849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HBH1523715.1"
                     /translation="MMDILIKNAIIVTVNKEREVIFDGALVVKDNKIADIGNSKEIES
                     KYTDVKKIIDAKGKVLFPGFINTHTHLFQTLLKGLGDDMVLKDWLETMTFPAANYLEP
                     KDTYDAAMLGCIEGLRSGITTMVDYMYPHSKPGLCDGIIDAYKELGIRGILGRGCMNT
                     GAQFGVHPGIMQDVETVEKDVRRLFEKHHNTENGRIKIGVAPAAIWSNSQEMLEMLWR
                     VAKEYDNALFTVHISETPFDREAAKELHGQYDIDVLEKLGILGPNVLMVHCVYLTEKD
                     MELAKKYDMKVSHNTASNMYLSSGVAPVPEMLKKGITVSLGVDGAASNNSQDMLELMK
                     LTALQHKVNKCDPLAMSAEKVLELATIDGARAIGMEDEIGSLEIGKKADLLIFNPMLS
                     PKAIPMHNPVSTLVYSSSMKNIESVIVDGNIIMEDSKILTTNEEKALKDAQDTAERLC
                     VRGTIKNRMEGHKWNSLY"
     gene            3323..3769
                     /locus_tag="KRL67_002850"
     CDS             3323..3769
                     /locus_tag="KRL67_002850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster binding
                     domain-containing protein"
                     /protein_id="HBH1523716.1"
                     /translation="MIIKCTINNKEKELSIRPDDFLADTLRENGYVSVKKGCDTGSCG
                     LCTVMVNDKAVLSCNYLTARVDGQHITTLEGVLDEAKKFGDFLADEGADQCGFCAPGF
                     IMMVLAMKRELQNPTEEEVIDYVNGNLCRCTGYKGQLRAIHKYLNT"
     gene            3808..4602
                     /locus_tag="KRL67_002851"
     CDS             3808..4602
                     /locus_tag="KRL67_002851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HBH1523717.1"
                     /translation="MITIKEYVVPKSLDEAYELLISRKNNIILGGCGFLKLGSKNIGS
                     AIDLKDLALDYINETDDSILIGADTSLRTLELNKVIKNYCNGVISNAVSNIVGVQFRS
                     GARVGASVFAKYGFSDLIPSLLVVDAKVKLYKKGVMNLADFLENELEKDILIEVILPK
                     KDAIGVFDSIRKCTGDFAVLNGAMLKEGSIYKIAIGARPRRAKIAYKASEILSLENDI
                     EKAGEVSSEELSFGSNIRGSKEYRKDMAKALVVRMYNSIGGECDGK"
     gene            4592..6883
                     /locus_tag="KRL67_002852"
     CDS             4592..6883
                     /locus_tag="KRL67_002852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HBH1523718.1"
                     /translation="MVNKEVRKIDSEAIVTGKPIYTEDLIFHKDVLTIKLLRSPHAFA
                     KIKNIDIKNALKVKGVVNIFTYKDVPNYRYSMVGESYPEASPHDQLILEDIVRYVGDE
                     VAIIAAEDERAAIKAMKLIKVEYEVMTPILDARESEGNKILIHEEDDLFCPFDFGLDA
                     TKNIVSREKMGKGDVEKEFKESDVIVEHSYMTQSQAHCMMETHRAYTYIDTNGRLVVT
                     SANQSAYHMRRQVSRALGIPLSKVRVIKPRIGGGFGGKNIAVTEIYASFVTWMTKRPA
                     KLIYTREETFAMTNTRHQMFFDIKIGADKEGNIKAIDMKALNNTGAYGDNGPSVCSES
                     GHNVLPTYNNVPAIKFDGRTVYTNLVPGGALRGYGATQGTFALDCAIDELAHKLNMDP
                     TEIKLKNIIKKQTEGGIMNFPIRSCALDECIIRGKKLIGWDEKYPVRDIGNNKVRAVG
                     MSVGTHGSGIANIDMAIVNMRMDEDGTYKLFSGSSDLGTGSDTILAQIAAKALNTSID
                     RISVYTGDTDMCPYDTGAYASCTTYVTGNATIKSAESLKQKILKVAQKKLNLPIDDLI
                     LYEDRVCYSEDVEKFVLLSELGKESVGGGNQEVLYSSESYGIQESAKPFLAGFAEIEL
                     DKTTGEFKVINYACSTDCGTVMNPALARIQVEGGVTQGIGLAMYEDPRHGSDGKLQTS
                     NLLQYKVPTKKDIGNIIVDFVDNYEPTGPYGAKSLGEIVIHTPAPAIANAIYNATGVR
                     IRELPITSEKVYMAMKKLNQGGK"
     gene            6884..8197
                     /locus_tag="KRL67_002853"
     CDS             6884..8197
                     /locus_tag="KRL67_002853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HBH1523719.1"
                     /translation="MIAIKSKYLISSADEIYTNYAVVVENDIIKDILPNEEVEIKYKN
                     TEEIIDKSDAIIMPGFVNGHMHQYGVLSRGIPANVHFTDFEGFLNDYWWPFIENRIGL
                     KEVKATTKASAIELIESGVVAFCDTLEAPNTEEGTLIEQGKILEEIGMKAVLSLESCE
                     RISFENGLRCLDENSNLIKWSRENSKLINGIMCTHTSFTCSDKFIKKAKEDAKKLNAP
                     MQFHLCESIYEPNYAEKHFGKKAVDYYNDLDILDETVLASQCVKVNDEEIDILKEKGV
                     KVVHMPLSNCEVGGGFSPVPKMIKKGIKVALGTDGYINDFFEVMRGAFLMHKSVEEDA
                     SVMPANLVFRMATENGAHVLGLQNSGKIAVGNKADIIVMEDEFKTPVTLDNIFDQIVV
                     QGKKEFISNVYIDGRHILKEKQLVDLDKKAIVKEMKEVACEFWKF"
     gene            8233..9318
                     /locus_tag="KRL67_002854"
     CDS             8233..9318
                     /locus_tag="KRL67_002854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HBH1523720.1"
                     /translation="MYNFLGKELKSPLIIGSGPLTYSAAGCKILSDAGAGAVVTKTIR
                     KERAINPAPHMVRNTTNALLNNEKWTDFEPEQWIDFEIPQMKRDGTVCIASVGHTIEE
                     SSELVEKVANAGADFIELVSYDYRDLIPMLKDAKKRVNIPVIVKLPPMIDEIGDFAKK
                     LEEAGADAITACDSVGPAFRIDIETGQPLLGGNGIGYLSGETIKPITLQRIYEIKKQV
                     NIPIIGLGGCVSGDDALEMIMAGADFVGICSVVILKGAQVISKIHDDLKSNLNRLGYN
                     TIENACGIVHNHMGDVNERLSFEFTYDEEKCTKCKMCERVCAYQARKLNDKMELNEEE
                     CRYCGLCISVCKPKALGRKISCSELNV"
     gene            9545..10552
                     /locus_tag="KRL67_002855"
     CDS             9545..10552
                     /locus_tag="KRL67_002855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH1523721.1"
                     /translation="MVMQVQEKILELLYTINEDVVSIKNDMSEVKQEINVLKEDMSEV
                     KQEVTVMKGDISEVKQEMTVMKGDMSEVKQEINVLKGDMSEVKQEINVLKGDMFEVKQ
                     EMTVMKEDMSEVKQEMTVMKEDMSEVRQEMTVMKEDMSEVKQEINVLKEDMSEVRQEI
                     NIMKEDMSEVRQEMTVMKEDTSEVKQEINVMKKDMSEVRQEITVMKEDVSEVKSEVNF
                     MQNKINNINEDMNGIKNEVSTANEKLYGIEIKIDSLECEDKSMKEIQIEQNNILGSLL
                     HNSEINKATHDNIEHNIAYIKGDTNSIKEDIAEIRRDLNLVELATSKNWSDIVRLKSV
                     K"
BASE COUNT         4267 a         1124 c         2160 g         3058 t
ORIGIN      
        1 gcagttgaaa aagctgattg ctatacagga aatactatag aaagtgaaag attcttccca
       61 ggttggttat atgacgaaga agatgaattt gttcaagcag catataaggg attaaaagaa
      121 gctggaatag attcagaaat aactcaatac tcattctgta caaatggaag tcactatgca
      181 ggtgaagcag gaataaaaac tataggattt ggtccttcta aggaaaatct tgctcacaca
      241 atagatgagt atatagagca agaacagtta tttataggaa cagaaggtta ctatggaata
      301 ttaaaatctg tatacggaaa ataatattta gttcacaata aattatttaa atatagtcta
      361 aaggtgctga atatagcacc tttagaaaat acaattttaa ataaagtttt acgatacttg
      421 gaaaatactt taaagcttaa aagttaggag gtatatatgg gaacaatact aagaggtgga
      481 actataatta cctctgataa gacttatata agtgatttaa ggatagaaga tgaaaagata
      541 gttgaaatag gaaataactt agaaataaat ggtgataagg ttattgatgc tacaggtaaa
      601 attgtaattc ctggtggaat agacacacat actcattttg atatgaatgc aggttctatt
      661 acaactgcag ataattttga aacaggaaca agagcagcta tagctggagg aactactaca
      721 atactagatt ttgcagaagc caatgagggg gaaaacctac tgcaaggtgt agaagcatat
      781 cataaaaaag caagtggaaa ttgttattgt gattatggtt ttcatatgac aattacatgt
      841 ttaaataaag atacatttaa tcatatggaa aaacttattt cagatgggat agtatctttt
      901 aagatgtata tggcatatga tggcatgaaa gtagatgatg gagcaatata cagggtttta
      961 aagaaagcaa gagatttagg ttgtatagtt gaatttcatt gtgaaaatgg ggatttacta
     1021 gatgtattga tagaagaaaa tatacaaaat ggtaatttag agccaaaata tcatccactt
     1081 acaagaccta acatagttga gaaagagtct gtttcaagac ttgctgatat aactaaatta
     1141 tctaattcaa aatcctatgt agtccactta agttgtaagg agtcacttga aactgtaaaa
     1201 acagctagag aaaaaaatat agatatgata gtagaaacat gtccacagta tctattatta
     1261 gaagacaatc tatataataa ggataaattt gagggtgcta agtatgtaat gtctcctcct
     1321 ttaagaaaaa aagaagacat aaattacctt tgggaaggat tggctagtgg agatattcaa
     1381 acagttggca cagaccactg ttcatttaac tttaaggggc aaaaagactt gggaattgat
     1441 gattttagta agataccaaa tggagcacct ggtgttgaac ataggctagc ccttttatat
     1501 acatatgggg tcttagaaaa tagaataagt gcaaataagt ttgtggaagt tacttctacg
     1561 aatgctgcaa aaatatttgg aatgtatcct aaaaaaggtg agatagctgt aggaagtgat
     1621 gcagatatag tcattttaaa tgttaataaa gaagaaacaa ttagttataa aactcaaaaa
     1681 caaaatgtag attatactcc atatgaagga tttaagatta aatgtaaagt tgaagatgta
     1741 tttttgagag gaaatcatgt agtacaaagt tgtaatgtta aagaacatcc aacaggacaa
     1801 tatattaaaa gaaaaattac taaataatgc ggggggaaat aagaatgatg gatatactta
     1861 ttaagaatgc cataatagta actgtgaata aagagaggga agttattttt gatggggcat
     1921 tagtagtaaa agataacaag attgctgata ttggaaattc taaagagata gaaagtaaat
     1981 atactgatgt taaaaaaatt atagatgcta aaggtaaagt tttattccca ggttttataa
     2041 atacacatac tcatttattc caaactttat taaaaggatt gggagatgat atggtgctta
     2101 aagactggtt agaaactatg acgtttccag ccgctaacta tttagagcca aaagatactt
     2161 atgatgctgc aatgcttgga tgtattgaag gtcttagaag tggtataaca acaatggttg
     2221 actacatgta tcctcatagt aagccaggtc tttgtgatgg aataatagat gcttataagg
     2281 aattaggaat aagaggaata cttggaagag gatgtatgaa tacaggagct caatttggag
     2341 ttcatcctgg aataatgcaa gatgtagaaa cggttgaaaa agatgtaaga agattgtttg
     2401 aaaaacacca taatacagaa aatggaagaa tcaaaatagg agtagctcct gcagcaatat
     2461 ggtcaaattc tcaagaaatg cttgaaatgt tgtggagagt agcaaaagaa tatgataatg
     2521 ctttatttac agtacatata tcagaaactc cttttgatag agaagcagca aaagaattgc
     2581 atgggcaata tgacatagat gttttggaga aattaggaat acttggacca aatgtgctta
     2641 tggtacattg tgtgtatcta actgaaaaag atatggaact tgctaaaaaa tacgacatga
     2701 aagtgtctca taatacagca agtaatatgt acttatcttc aggtgttgca ccagtaccag
     2761 aaatgcttaa aaagggaata acagttagtt taggagtaga tggagccgct agtaataact
     2821 ctcaagatat gcttgaactt atgaaattaa cagcacttca acataaagtt aataagtgtg
     2881 acccattagc aatgtcagca gaaaaagttc ttgagttggc aactatagat ggagcaagag
     2941 ctattggaat ggaagatgag ataggttcat tggagatagg taaaaaagca gacttactaa
     3001 tatttaatcc aatgctttca ccaaaagcga tacctatgca taatccagta tctacattag
     3061 tatattcttc tagtatgaaa aatatagaga gtgttatagt agatggaaat ataataatgg
     3121 aagattcaaa aatattgaca actaatgaag agaaagcatt aaaagatgct caagatacag
     3181 ctgagagact ttgtgtaaga ggaactataa aaaatagaat ggaaggtcat aaatggaata
     3241 gcctttatta ataaatgtaa tttattcata aaaaagtata gtaaaacttt agcttaaaaa
     3301 tatgagtttt gaggtgattt atttgattat caaatgtacc ataaataata aagaaaaaga
     3361 attaagtata agaccagatg attttttagc agatacatta agagaaaatg gatatgtaag
     3421 tgttaaaaaa gggtgtgaca caggctcatg tggcttatgt acagtgatgg taaatgacaa
     3481 ggctgtgctt tcatgtaatt atcttactgc tagagtagat ggtcagcata ttacaacact
     3541 tgagggtgtc ttggatgaag caaagaagtt tggagatttt cttgcagacg aaggggctga
     3601 ccagtgtgga ttttgtgctc ctggatttat aatgatggtc ttggcaatga aaagagagct
     3661 tcaaaatcca acagaagaag aagttataga ttatgtaaat gggaaccttt gtagatgtac
     3721 aggatataaa ggtcaattaa gagcaataca taaatattta aacacttaga cttttaagtg
     3781 aaaattaagt tgatatgggg agagagtatg ataactataa aggaatatgt agttccaaaa
     3841 agtttagatg aagcttatga actgctaata tcaagaaaaa acaacataat acttggagga
     3901 tgtggatttt taaaactagg tagtaaaaat attggttcag caatagattt aaaagactta
     3961 gctttagatt atataaatga aactgatgat agcatattaa ttggagcaga cacaagttta
     4021 agaactttag aattaaataa ggttattaaa aattactgta atggagtaat ttcaaacgct
     4081 gtttctaata tagtaggagt tcaatttaga agtggagcta gagtaggagc aagcgtattt
     4141 gctaagtatg gattttcaga tttaatacct tcattactag tggtagatgc aaaggttaag
     4201 ttatataaaa aaggggttat gaacttagct gattttttag aaaatgaact tgaaaaagat
     4261 atattaatag aagttatatt acctaaaaaa gatgctatag gtgtatttga ttctataaga
     4321 aaatgtacag gtgattttgc agtattaaat ggagcaatgt taaaagaagg aagtatttat
     4381 aaaattgcaa taggtgctag accaagaaga gcaaaaattg cctataaagc aagtgaaata
     4441 ttaagtttag aaaatgatat agaaaaagct ggtgaagtat caagtgaaga actttcattt
     4501 ggaagtaata taagaggaag taaagaatac agaaaagaca tggcaaaagc cttagtagtg
     4561 agaatgtata atagtattgg aggtgagtgt gatggtaaat aaagaggtaa gaaaaataga
     4621 tagtgaagcc attgttactg gaaaaccaat atatacagaa gatttaattt ttcataaaga
     4681 tgttttaaca attaaacttc ttagaagtcc acatgctttt gcgaagataa aaaacataga
     4741 tataaaaaat gcattaaagg taaaaggtgt agtaaacata ttcacttaca aagatgttcc
     4801 aaattataga tattcaatgg ttggggaatc atatccagaa gcatcacctc atgaccaatt
     4861 aatacttgaa gatatagtta gatatgtagg agatgaagtc gctataatag ccgctgaaga
     4921 tgaaagggct gctattaaag ctatgaaact tataaaagtt gaatatgaag taatgactcc
     4981 tatattggat gcaagagaat cagaaggaaa taaaatttta atccatgaag aagatgattt
     5041 gttttgtcca tttgattttg gattggatgc tacaaaaaat atcgtatcga gagaaaaaat
     5101 gggtaaaggg gatgtagaaa aagagtttaa agaaagtgat gtaatagtag aacattctta
     5161 tatgactcaa tcacaagccc attgtatgat ggaaactcat agagcttata cgtatataga
     5221 tacgaatggc agattagtag taacttctgc aaaccaatca gcttatcata tgagaagaca
     5281 ggtttctaga gcattgggaa tacctctttc aaaagtgaga gtaataaaac caagaatagg
     5341 tggaggtttt ggtggaaaaa acatagctgt aacggagata tatgcatcat ttgtaacttg
     5401 gatgacgaaa agaccagcaa aactaattta tactagagaa gaaacttttg caatgactaa
     5461 tactagacat cagatgtttt ttgatataaa aattggggca gataaagaag gaaacataaa
     5521 agctatagat atgaaagcat tgaacaatac aggcgcttat ggtgataatg gaccttcagt
     5581 ttgctctgaa tcaggtcata atgtacttcc aacatataat aatgtacctg caataaaatt
     5641 tgatgggaga actgtatata ctaatttagt tccaggggga gcattgagag gatatggagc
     5701 aacacaaggt acatttgcac ttgattgtgc aattgatgaa ttagcgcata aacttaatat
     5761 ggacccaaca gaaataaagt taaagaatat tataaaaaaa caaactgaag gcggaataat
     5821 gaattttcca ataagaagtt gtgcacttga tgaatgtata attagaggta aaaaacttat
     5881 tggatgggat gaaaaatacc ctgttaggga tattggaaac aataaggtga gggctgttgg
     5941 aatgtctgtg ggaactcatg gttctggtat agccaacata gacatggcaa tagtaaatat
     6001 gaggatggat gaagatggaa cttataagtt attttctggg tcttcagact taggaactgg
     6061 ttctgacaca atacttgcac aaatagctgc aaaagcattg aatacatcta ttgacagaat
     6121 ttccgtttat acaggagata cagatatgtg tccatatgat actggtgcat acgcttcttg
     6181 tactacttat gtaactggaa atgctacaat aaaatctgct gaaagtttaa aacaaaagat
     6241 attaaaagta gcacagaaga aactcaattt accaatagat gatttaatac tatatgaaga
     6301 cagagtttgt tatagtgagg atgtagagaa gtttgttttg ttaagtgaat taggaaaaga
     6361 atctgttggt ggtggaaatc aagaagtttt atattcatct gaatcttatg gtatacaaga
     6421 gagtgctaaa ccattcttag caggatttgc tgaaatagag ttagataaaa caacagggga
     6481 gtttaaagta ataaattatg cttgttctac agattgtggt acagttatga atcctgcact
     6541 tgctagaata caagttgaag gtggtgtaac tcaaggtata gggcttgcaa tgtatgaaga
     6601 ccctagacat ggttctgatg gaaagcttca aacctctaat ctattacaat acaaagttcc
     6661 aacaaaaaaa gatataggaa acataattgt tgattttgta gataattatg agccaacagg
     6721 tccatatgga gctaaatctt taggagaaat agttattcat acacctgcac cagcaatagc
     6781 aaatgcaata tacaatgcca caggagttcg tataagagaa cttcctataa cttctgagaa
     6841 agtttatatg gccatgaaaa aattaaatca gggaggtaaa taaatgattg ctattaaaag
     6901 taaatactta atatcttctg ctgatgaaat atatacaaat tatgctgttg tagtagaaaa
     6961 tgacattata aaagatatat tgccaaatga agaagttgaa ataaaataca aaaatactga
     7021 agaaataata gataaatcag atgctataat aatgccagga tttgtaaatg gtcatatgca
     7081 tcaatatgga gttctttcta ggggaatacc agcaaatgta cattttactg actttgaagg
     7141 tttcttaaat gactattggt ggccatttat agaaaacaga attggattga aagaagtaaa
     7201 ggcaacaact aaagcatcag caatagaact tatagaatct ggtgtggtag ctttttgtga
     7261 taccttagaa gctccaaata cagaagaagg aactttaatt gagcaaggaa aaatactaga
     7321 agagataggt atgaaagcgg ttttatcatt agaaagttgt gagagaataa gctttgaaaa
     7381 tgggcttaga tgtttagacg aaaattctaa tttaataaag tggagtagag aaaatagtaa
     7441 attaataaat ggaataatgt gtacacatac aagcttcaca tgttcggata aatttattaa
     7501 gaaagctaaa gaagatgcaa agaaattaaa tgcaccgatg caatttcatt tatgcgagag
     7561 tatatatgag ccaaactatg ctgaaaaaca ttttggcaaa aaagcagttg actactataa
     7621 tgatttagat atattggatg aaactgtatt agcatctcag tgtgtaaaag taaatgatga
     7681 agaaatagat atattaaaag aaaaaggtgt aaaagtagtt catatgcctc ttagcaactg
     7741 tgaagtaggt ggaggttttt ctccagtacc aaaaatgatt aaaaaaggca taaaagtggc
     7801 actgggaact gacggatata taaatgattt ttttgaagtt atgagaggtg catttttaat
     7861 gcataaatca gttgaagaag atgcatcagt aatgccagca aatttagtat ttagaatggc
     7921 aacagagaat ggtgctcatg tattaggact tcaaaattct ggtaaaatag cagttggtaa
     7981 taaggctgat ataatagtta tggaagatga atttaaaact cctgtgactc ttgataatat
     8041 atttgaccag atagtagttc agggtaaaaa agaatttata tcaaatgtat acatagatgg
     8101 tagacatatt ttaaaagaaa aacagttagt tgatttagat aaaaaagcta ttgttaaaga
     8161 aatgaaagaa gttgcttgtg aattttggaa attttaacga atgtaaatat atatgaaaag
     8221 gggaagcaga caatgtataa ttttttaggt aaagaattaa aatcaccact tataataggt
     8281 tcaggaccat taacatatag tgcagctgga tgcaagatat taagtgatgc aggagcaggg
     8341 gcagttgtga ctaagactat aagaaaagaa agagctataa atccagcacc acatatggtt
     8401 agaaatacaa ctaatgctct tttaaataat gagaaatgga cagatttcga gccagagcaa
     8461 tggatagatt ttgaaatacc acaaatgaaa agagatggga ctgtctgtat agcttcagta
     8521 ggtcatacaa ttgaagaaag tagcgaatta gtagagaaag ttgcaaatgc aggagctgac
     8581 tttattgaat tagtatctta tgactataga gatttgatac caatgcttaa agatgcaaag
     8641 aaaagagtaa atatacctgt aatagttaaa cttcctccta tgatagatga gataggcgat
     8701 tttgctaaaa aattagaaga agcaggagca gatgcaataa ctgcttgtga ttctgtagga
     8761 ccagcattta gaatagacat agaaacaggg caacctttat taggtggaaa tggaatagga
     8821 tatttaagtg gagaaactat caagccaata actttacaaa gaatatatga aataaaaaaa
     8881 caagttaata taccaataat tggattaggt ggatgtgtaa gtggagatga tgccttagaa
     8941 atgattatgg caggggctga ctttgttggt atatgttcag tagtaatctt aaaaggtgca
     9001 caagtaatat ctaaaataca tgatgattta aaatctaatc ttaatagatt aggatataat
     9061 actatagaaa atgcttgtgg tatagtacat aatcatatgg gagatgttaa tgaaagactt
     9121 agctttgagt ttacttatga tgaggaaaaa tgtactaaat gtaagatgtg tgagagagtt
     9181 tgtgcatatc aagcgagaaa attgaatgat aagatggaat tgaatgaaga agagtgtaga
     9241 tattgtggac tttgtatatc agtttgtaaa ccaaaagcac ttggaagaaa aatttcatgt
     9301 tcagagctga atgtatagat tattttataa aaaataagtt ttaaataaaa ataagaaagg
     9361 ctatctcaaa gagataagcc ttttttgttg ttgaccttat ttttgtttat ttgtagttat
     9421 tttagatatt aattagtttt gaagtaataa agtgaatcaa ttttgtttaa ttaaaatttt
     9481 aataattctg atatatgtta acattgtata gtatttgtgt aaaatgttat aataattaat
     9541 agggatggtg atgcaagtgc aagagaagat tttagagtta ttatatacta taaatgaaga
     9601 tgtagttagc ataaaaaatg atatgtccga agtaaaacaa gaaataaatg tgttaaaaga
     9661 agatatgtcc gaagtaaaac aagaagtgac tgtaatgaaa ggagatatat cagaagtaaa
     9721 acaagaaatg actgtaatga aaggagatat gtccgaagta aaacaggaaa taaatgtatt
     9781 aaaaggagat atgtccgaag taaaacagga aataaatgta ttaaaaggag atatgttcga
     9841 agtaaaacaa gaaatgactg taatgaaaga agatatgtcc gaagtaaaac aagaaatgac
     9901 tgtaatgaaa gaggatatgt ctgaagtaag acaagaaatg actgtaatga aagaagatat
     9961 gtctgaggta aaacaggaga taaatgtatt aaaagaagat atgtccgaag taagacaaga
    10021 gataaatata atgaaagaag atatgtctga agtaagacaa gaaatgactg taatgaaaga
    10081 agatacatca gaggtaaaac aagagataaa tgtaatgaaa aaagacatgt ctgaagtgag
    10141 acaagaaata actgtaatga aagaagatgt ttccgaagtt aaatcagaag taaactttat
    10201 gcaaaataaa ataaataaca ttaatgaaga tatgaatgga attaagaatg aggtatccac
    10261 agcaaatgaa aaattatatg gaattgaaat taagattgat agtctagaat gtgaagataa
    10321 atcaatgaaa gagatacaga tagaacagaa taatatttta ggaagtttat tacataattc
    10381 tgaaataaat aaagctactc atgataatat agaacataat atagcatata taaaaggtga
    10441 tactaattct ataaaagagg atatagctga aataagaaga gacttaaatt tagtagagct
    10501 agccactagt aaaaattgga gtgatattgt cagactaaaa tcagtaaagt agtattttaa
    10561 ttcactaatt tatcatccac aacctaagaa ttttatataa aagaattta
//
LOCUS       DAEPXF010000136        10604 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain HB27
            SAMN13376389-rid12479423.denovo.143, whole genome shotgun sequence.
ACCESSION   DAEPXF010000136 DAEPXF010000000
VERSION     DAEPXF010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13376389
            Sequence Read Archive: SRR10518432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10604)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10604)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001069624.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001069624.1
            Genome Coverage       :: 315x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/15/2021 01:54:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,165
            CDSs (total)                      :: 4,076
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 89
            rRNAs                             :: 10, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 10 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 19 of 69
            Pseudo Genes (incomplete)         :: 44 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 10 of 69
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10604
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13376389-rid12479423.denovo.143"
                     /strain="HB27"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="China: Hebei"
                     /collection_date="2017"
     gene            complement(36..767)
                     /locus_tag="KRL67_002856"
     CDS             complement(36..767)
                     /locus_tag="KRL67_002856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HBH1523722.1"
                     /translation="MKFSHKLNNLSHAEKHIFYYIDSSIHTVKDLSLTALADINNVST
                     TTIIRMCSKIGLSGYSELKHILKDLDKESTSISSGYHVSNIIDNLNQNLSNLNLTNIN
                     KLAKNIKSANRIIIVAVGLSKPIGEYFSKLLMQANKNSFYVYESHMIDLLDQTTEKQD
                     LVIFVSNSGETRTLITACEKFRYKNLNTAAIINSPNSTLSMLVDIPINTCSQKLNISG
                     YDTTPRSTILVIIDILFETYLNIIE"
     gene            920..2890
                     /locus_tag="KRL67_002857"
     CDS             920..2890
                     /locus_tag="KRL67_002857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBH1523723.1"
                     /translation="MNLAKMTNKNLIVLDVSVATKEEAIRLLIDKLYKENIISSKEKF
                     FDTVMEREKHSPTGLERGLAIPHGKCDSVNRSAFAVARLNNKINDWESIDESNEVDLV
                     FLLAIPKSEKETTHLKLLSELSVALMDENFYNDLSISKNADEFLKNLNRSDKEEKDTN
                     KYDKTVLVVTACAAGIAHTYMSAEALEKVGKEMGIKVISEKQGASGIEDRHTKEQIQK
                     ADGVIFACDVAVKNIERYQGKSFVKVRVAEPLKHSKNLLNKVLQNPDGKVEVLSNEEA
                     FTSTYELKQGTLKEMMEAVMTGISYMIPVIVSAGLMMGIAKLTAVAIGQIDNMGTFIE
                     SGNTFYELLGYLDMFGSMIFKFIYPIFSAYVAYSIADRPALVPGFVGGAFAGGLHFTF
                     WGVEGGVPSGFIGALILGLVAGYVTRFLNENIKLNKNLQAMKPMFLLPGITMLVIFLL
                     NFYAVDPAFGRVNAFIANIIESFGTASTIALTVVIAACTAFDLGGPVNKAAGAIAIGL
                     AADGVFPLTARVLSIVIPPIGLGLATVLDKIIVKRRVFDDNLRVVGNSSIILGLIAVS
                     EGAIPFMLKNPLITIPINILGAIIGSITAVMLGAIQWNPLPAIWGWPLVENLWAYLVG
                     LIVGTLFIALANIFIRFNIIKKQKKNDMNKTA"
     gene            2996..5674
                     /locus_tag="KRL67_002858"
     CDS             2996..5674
                     /locus_tag="KRL67_002858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HBH1523724.1"
                     /translation="MEEDMNNRKKVYVVTHSHWDREWYFTIEDSNILLSENMPYLMDV
                     LEKDMDYTSYTFDAQLSVVEELVKLYPEEKERLKKLVSDKRIFVGPWYTQTDSILVNK
                     ESIIRNLLYGTRLGNKYGHSMKVGYLPDIFGQNAYLPSIFNGFDIEYSILQRGIYIDD
                     LKGNLNFKWVSPDNESVRANNIFLGYGPGKFLSSDNQYIQDKLLPMLEKLESLNGDCD
                     NLLLPCGGDQVLVRKQFPQTVKELNEKQDKYEFVLSDYETFMKDTWINDFDNKIHGEL
                     IACEKSRIHNTIKSQRYDIKYLNTLVENKILYVLEPLSVIGKFSGISYKSRWLDIMWK
                     LIFDAHAHDSIGGCNSDETNRDIVTRLKKVNAMCDDIINIVKKQITMAISKYLNRDNI
                     LVVFNTKNKCMNEIIETVVFTKEKDFTINTIDKEIVEFTINNQEVISGGKQILVTAEG
                     EKEVELPGYYRTEIRLANVNVKPMGFTTLVVNQNSKEVADILVASNDRSIENDLYKLV
                     FNNNNLLLINKYTNKKIDNIVYFENCGDYGDSYDFSPLDNDKDILASKAELLEVSKSN
                     LVQTMKLKHSIELPFDIDERKLNKKSTILEIITRIEIRKGENFIRVSHDIENTVKDHR
                     VRVVYNTNIKSKVSLSDQGFSAITRQNCNRYLDNWKERKFAEKPVCIYGLENFVALRE
                     NSNTFALVTKGIKEYEILEDSKIALTLYRSVGLLGRDNLAWRPGRASGINNKVMYTKD
                     AQLFNKKLKFEYAIYYDKSDEDVATLFDVTDKFINKYTTYQNQDLNLFEERLERFEIP
                     KYHTINLSSYSLFNIDNDDVFMSVCKESYEEDGVIVRLFNPTNEAKEVSINSEYIKDI
                     KITNLYERELDTLDSNVQIKAKGYITLKLLGGGYNE"
     gene            5667..7361
                     /locus_tag="KRL67_002859"
     CDS             5667..7361
                     /locus_tag="KRL67_002859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphorylase"
                     /protein_id="HBH1523725.1"
                     /translation="MNKTILLQDIEQRLKNIYKEKYKPKFFEMMKSTIEKFEDKDFDK
                     VDAISEKNVYLITYGDSIYEEGIPTIHTLKKFLNNKVGYTITDVHILPMFEYTSDDGF
                     SVVDYMKIDKNLGNWESIKNLSKDYRLMYDFVANHISKSSIWFQGYLRGEEKYQNYFI
                     PEDENFDYSNVIRPRTSPIIHKYQGKNGVKTAWTTFSEDQIDINVKHFPVLVETTEVL
                     LNYALNGASSIRLDAIGFLWKESGTTCIHLPQTHMIIQLWRVMLDYFKKDTQIISETN
                     VPHLENISYFGDTTNEAHMVYQFTLPPLTLHTFTTHDSTKLTKWASTIDKVSDNATFF
                     NFLSSHDGIGMRPTEGILTEEENKRLVDKTIINGGRVSYKDNPDGTKSVYELNINYLD
                     ALLNKDEDIDEDIQIQKILSAHALLLSFIGVPAIYYHSLLGSKNYYEGVEKSGINRRI
                     NREKLEYNRICYELEHDSRRNKIFTQLKNMIDIRKGESAFSPYANQNVLDFGREIFAL
                     ERENKETNEKVVFITNVTPNEKIIDCGFNGINILENTNIEGKLTLGAYKFAWIKIK"
     gene            7618..8328
                     /locus_tag="KRL67_002860"
     CDS             7618..8328
                     /locus_tag="KRL67_002860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBH1523726.1"
                     /translation="MINIGICDDELHYRLKIKDILKKVLSSYTMDYNIHEFSSGKELL
                     CNYPKNLDILIIDIQMEILNGIDTSRKIREFDENLEIIFMTSFPEFMQEGYEVKAYRY
                     ILKPINEKKITKNILPCIDEIMKKRNNYLTIKVKNYVDRIKIDSIIYIETDRPNIVIY
                     THDNTYTTKMSISKIEKILSEFGFFRCHNSYIINLKLVQSMNGNSVVINGKSIPVSKY
                     RVKGLKLAITNILGDIIC"
     gene            8322..9638
                     /locus_tag="KRL67_002861"
     CDS             8322..9638
                     /locus_tag="KRL67_002861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HBH1523727.1"
                     /translation="MLETLNINLWDIINIITGCIDWIIFFLVMSILGKKNISFRKYTI
                     YMTLVLVSMSLVNFMSIFPNFKIIICIVIGSLFYIASYKESIYKCILITLLFWLGLMM
                     GEGLAIGIVVVINQLNNIDIILNGNLFRLESIVISKIFLFIIFMSIKYFKLSIEFKPK
                     DMVLIGLPILSNIVSLLLIFGYNLKNNFVSDDNIFVIVLSTLLIVSSSIIILIVIGKI
                     VQDDKVKLEYELINERIKIDYKSYESINEIHDKLKYVYHDLKNHMICIKNYDTKEEII
                     SYVNNLENKINDFEYFRNTGNKTLDIILGEKIYLCKKYNIEFEDSINISKLQFIQDVD
                     ICAIFANALDNAIEACMKMNNNTEKRIEVKATYVNGFAIIKFINTKVNDIKFIDERIK
                     TSKDNNRVHGIGLASIKYMVSKYDGELIVNYSDNEFILKIMIPIKA"
     gene            9651..9818
                     /locus_tag="KRL67_002862"
     CDS             9651..9818
                     /locus_tag="KRL67_002862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic lactone autoinducer peptide"
                     /protein_id="HBH1523728.1"
                     /translation="MKKIALSLLKSMSTLSLGISRLSANTASSWIAHQAEEPQALKKL
                     KKNNTSTKFSL"
     gene            9858..10442
                     /locus_tag="KRL67_002863"
     CDS             9858..10442
                     /locus_tag="KRL67_002863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory gene regulator B family protein"
                     /protein_id="HBH1523729.1"
                     /translation="MFKSLSYKFANILVRNEVIEDEDFEIYRYGFETLVYFIINISVA
                     LLIGIALNKFIQTIIFLVCYCTLRQFTGGYHARNYTECTITFALIYISIILVTKNIDI
                     YKFKYILILLLLLSTVIIHKVAPLEHRNKPLSIYEKKNYRNIIKKITLSIIIVVTISL
                     IFNIMSEYIIYSSLAVFLITILLLVQIIINFFKK"
BASE COUNT         4228 a         1074 c         1797 g         3505 t
ORIGIN      
        1 agaagacatt tttacttata actatatctt ttttattatt caataatatt tagataagtt
       61 tcaaataata tatctataat aactagtata gtagaccttg gagtagtatc gtatccagaa
      121 atattaagct tttgagaaca tgtatttata ggtatatcta ctagcataga tagtgtagag
      181 ttagggctat ttattatagc tgctgtgttt aagtttttgt atctaaattt ttcgcatgct
      241 gtaattagtg tgcgtgtttc tccactatta gatacaaata ttactaagtc ttgtttttct
      301 gttgtttggt ccaacaagtc aatcatatga gactcatata cataaaaact atttttatta
      361 gcttgcatta gcagttttga gaagtattct ccaattggct ttgagagccc tactgcaact
      421 attattattc tattggccga tttaatattt ttagctagtt tatttatatt tgttaaatta
      481 agatttgata gattttgatt taagttatct attatattac taacatgata accactagat
      541 atagatgtag attccttatc taaatcttta agtatatgct ttaattcaga gtaacctgat
      601 aatcctatct tagaacacat tcttattatt gtggtagtgc ttacattatt tatatcagct
      661 aaagctgtaa gtgataaatc ctttactgta tgtatgctcg aatctatata ataaaatata
      721 tgtttctcag catgactaag attattcaat ttatgtgaaa atttcattat caaactacta
      781 atattaatca ccacctttgt atatagtaaa aaagtacaaa tgtacttttt atgtataaga
      841 aaatgttttt catctaatat ataataatat tataagataa ataagcattt aagtaaatgt
      901 attaatagga gggtgagata tgaatttagc taaaatgacc aacaaaaact taatagtgtt
      961 agatgtaagt gtagctacaa aggaagaagc tataaggtta ttgatagaca agctttataa
     1021 agaaaatatt attagttcaa aagaaaaatt ctttgatact gtaatggaaa gagaaaagca
     1081 ttctccaact ggactagaaa gagggttagc tataccacat ggtaaatgtg actctgtaaa
     1141 taggtcagct tttgctgtag ctagattaaa caataaaata aatgattggg aaagtataga
     1201 tgaaagtaat gaagtggatt tagtattttt attagctata cctaagtctg aaaaagagac
     1261 tactcatttg aagttattat ctgaattatc tgtagcttta atggatgaaa atttctataa
     1321 tgatttaagt atttctaaaa atgctgatga gtttttgaag aatttaaatc gtagtgataa
     1381 ggaagaaaaa gatacaaata aatatgataa aactgtattg gtagtaactg cttgtgcagc
     1441 agggatagct catacatata tgtctgctga agctttagaa aaagtaggta aagaaatggg
     1501 gataaaagta atatcagaaa agcaaggtgc tagtggtata gaagataggc atacaaaaga
     1561 gcaaatacaa aaagcagatg gggtaatatt tgcttgtgat gttgctgtta aaaatataga
     1621 aagatatcaa ggaaagtcat ttgttaaggt tagagtagca gagcctttaa aacattctaa
     1681 aaatttactt aataaagtac ttcaaaatcc agatggaaaa gttgaagttt taagcaatga
     1741 agaagctttt acatctacat atgagttaaa acagggtaca ttaaaagaaa tgatggaagc
     1801 tgtaatgaca ggtatatcat acatgattcc tgtaatagtt tcagcaggtc ttatgatggg
     1861 gatagctaag cttactgctg tggctatcgg tcaaattgac aatatgggaa cttttataga
     1921 aagtggaaat acattttatg aattactagg atatttagat atgtttggaa gtatgatatt
     1981 taagtttata tacccaatat tttcagctta tgtagcatat tctattgctg atagaccagc
     2041 tttagtacca ggatttgtag gtggtgcatt tgcgggagga cttcatttta ctttctgggg
     2101 tgtagaaggt ggagtaccat caggatttat aggagcttta atattaggtt tagttgcagg
     2161 ttatgttact cgatttttaa atgaaaacat aaagttaaac aagaatttac aagctatgaa
     2221 gccaatgttc ctattaccag gtataacaat gttagtaata ttcttattaa acttctatgc
     2281 tgtagaccct gcatttggta gagtaaatgc atttatagca aatataatag aatcttttgg
     2341 cactgctagt acaatagcat taactgtagt tatagcagct tgtactgcat ttgacttagg
     2401 tggaccagta aacaaggcag caggtgctat agctataggt cttgcagctg atggtgtatt
     2461 tccattaaca gctagagtat tatctatagt aataccacca ataggattag gtttagctac
     2521 tgttttagat aagattatag ttaagaggag agtatttgat gataatttac gagtggtagg
     2581 aaactcttct ataatacttg gactaatagc tgtgagtgag ggggcgatac ctttcatgct
     2641 aaaaaatcct ctaataacaa tacctataaa tatattaggt gcaataatag gctctataac
     2701 agcagtaatg ttaggtgcta ttcaatggaa tccattgcca gctatatggg gatggccttt
     2761 agtagaaaat ttatgggcat acctagtagg tttaatagta ggtacattat ttatagcatt
     2821 agctaatata tttataagat tcaatattat aaaaaaacaa aagaaaaatg atatgaataa
     2881 aacagcataa taataattgg gaattattca gataaattta actagaataa ttcccatttg
     2941 tacaagtgga tatttaataa aattatttag aaataattaa aaaagatgat tttctatgga
     3001 ggaagatatg aataatagga aaaaagtata tgtagtaact cactctcatt gggatagaga
     3061 atggtacttt actatagaag attcaaatat actattaagt gaaaatatgc cttatttaat
     3121 ggatgtactt gaaaaggata tggattacac ttcttacact tttgatgcgc agttatctgt
     3181 tgttgaagaa ttagtaaagc tatatcctga agaaaaagaa aggcttaaga agttagtaag
     3241 tgacaaaaga atatttgtag gtccttggta cactcaaaca gattctatac ttgtaaacaa
     3301 ggaatctata ataagaaatc ttttgtatgg tacaagactt ggaaacaagt atggacatag
     3361 tatgaaggta ggatatcttc ctgatatatt tggacaaaat gcatatttac catctatatt
     3421 caatggtttt gatatagaat acagtatatt acaaagggga atatacatag atgatttaaa
     3481 gggaaattta aactttaagt gggtttctcc tgataatgaa agtgttagag ctaacaatat
     3541 attcttagga tatggaccag gtaagttttt atcatctgat aatcagtata tacaagataa
     3601 gttactacct atgcttgaaa aattagaaag tttaaatgga gattgtgaca atttactttt
     3661 accatgtggt ggagatcagg ttttagtaag aaagcaattc ccacaaactg taaaagaatt
     3721 aaatgaaaag caagataagt atgagtttgt actatcagat tacgaaactt ttatgaagga
     3781 tacttggatt aacgattttg ataataagat acatggagag ctaatagcat gtgaaaagtc
     3841 tagaatacac aatactataa agtctcaaag gtatgatata aaatacttaa acactttagt
     3901 agaaaataag attctttatg tattagaacc attatctgta ataggtaaat tttctggaat
     3961 aagttataag tctagatggt tagatattat gtggaagtta atatttgatg cacatgcaca
     4021 tgatagtata ggtggatgca actcggatga gacaaataga gacatagtta caagacttaa
     4081 aaaggttaat gcaatgtgtg atgatattat aaatattgtt aagaaacaaa taacaatggc
     4141 tataagtaaa tatttaaata gagacaatat attagttgta tttaacacta aaaataaatg
     4201 tatgaatgaa ataatagaga ctgtagtatt tactaaggaa aaagacttta caataaatac
     4261 tatagataaa gaaattgtag aatttactat aaataaccaa gaagttataa gtggtggtaa
     4321 gcagatatta gtaactgctg aaggtgagaa agaagtagaa ttacctggat actatagaac
     4381 tgaaatcaga ttggctaatg taaatgttaa acccatggga tttactacat tagtagttaa
     4441 tcaaaatagc aaagaagtag ctgatatatt agttgcatct aatgatagga gtatagaaaa
     4501 tgatttatat aaattagtat tcaataacaa taacttacta ttaattaata agtatactaa
     4561 caaaaaaata gataatatag tttattttga aaattgtggt gactatggag attcatatga
     4621 tttctctcca ttagataatg ataaagatat actagcaagt aaggcagagt tattagaagt
     4681 atcaaagagc aatttagtac aaactatgaa gttaaagcac agtatagaat tgccttttga
     4741 tatagatgaa agaaaattaa ataaaaagtc aactatatta gaaataataa ctaggataga
     4801 aataagaaag ggtgaaaact ttataagagt ttcacatgat atagagaata ctgtaaaaga
     4861 ccatagagta agagttgtat acaatacaaa tataaaatca aaagtgtcat taagtgacca
     4921 aggattctct gccataacaa ggcaaaactg caataggtac ttggataatt ggaaagaaag
     4981 aaagtttgct gaaaagccag tatgtatata tggcttagag aactttgtag ctttaagaga
     5041 gaatagcaat acttttgcat tagtaacaaa agggataaaa gaatatgaaa tattagagga
     5101 cagcaaaata gcacttactt tatatagaag tgttggttta ttgggaagag ataacttagc
     5161 ttggagacca ggtagagctt ctggtataaa caacaaggta atgtatacta aagatgcaca
     5221 actttttaac aaaaaactaa agtttgagta tgctatatac tatgataagt cagatgaaga
     5281 tgtagcaaca ttatttgatg ttactgataa atttataaat aagtatacta cttaccaaaa
     5341 tcaagatttg aatttatttg aagaaaggtt agaaagattt gaaataccta aatatcatac
     5401 tataaactta tcaagctata gtttatttaa tatagataat gatgatgtat ttatgagtgt
     5461 gtgtaaagaa tcctatgaag aagatggagt aatagtaaga ttgtttaatc caacaaatga
     5521 agctaaagaa gtaagcataa atagcgaata tataaaggat ataaagataa ccaatttata
     5581 tgagagggag ttggatactt tggattctaa tgtacaaata aaagctaaag gatatataac
     5641 tcttaagtta ttagggggag gatataatga ataagacaat actgttacaa gatatagaac
     5701 aaagacttaa aaatatatat aaagaaaagt acaagccaaa gttctttgaa atgatgaaat
     5761 ctactattga gaagtttgaa gataaagatt ttgacaaggt agatgctata tctgaaaaga
     5821 atgtatattt aataacttat ggagatagta tatatgaaga ggggatacct actatacata
     5881 ctttgaaaaa attcttaaac aataaagtag gatatactat aactgatgtt catatacttc
     5941 ctatgtttga atacacttca gatgatggat tttctgttgt tgattatatg aaaatagata
     6001 aaaatcttgg aaactgggag agtataaaaa atttgtctaa ggattacaga ttaatgtatg
     6061 attttgttgc aaatcatata tcgaagagca gtatatggtt tcaaggatac ttaagaggag
     6121 aagagaagta tcaaaattat tttataccag aagatgaaaa ctttgattac tcaaatgtaa
     6181 taagacctag aacttctcca ataatacata agtaccaagg aaaaaacggt gtaaagactg
     6241 cttggactac attcagtgaa gaccaaatag atataaatgt taaacatttc ccagtacttg
     6301 tagaaactac tgaagttttg cttaattatg cattaaatgg tgcaagttct ataaggcttg
     6361 atgctatagg attcttatgg aaggagtctg gtactacttg catacattta ccacaaactc
     6421 acatgataat acaactatgg agagtaatgc ttgattactt taagaaagat actcaaataa
     6481 tatctgagac taacgtgcct catctagaaa atataagcta ttttggtgat actacaaatg
     6541 aagcacatat ggtatatcaa tttacattac cgccattaac gcttcataca ttcactacac
     6601 atgattctac caagcttaca aagtgggctt cgactataga taaggtatct gataatgcta
     6661 cgttctttaa cttcttatca agtcatgatg gaataggaat gagacctaca gagggtatac
     6721 ttacagaaga ggaaaataaa agattagtag ataagactat aataaatggt ggtagagtat
     6781 catacaagga taatccagat ggaactaagt ctgtatatga gttaaatata aactatttag
     6841 atgccttatt gaataaagat gaagatatag atgaggatat ccaaatacaa aagatattat
     6901 cagcacatgc attattatta tcatttatag gtgtaccagc tatatactat cattctctat
     6961 taggctctaa aaactactat gaaggagtag agaaatcagg tataaacaga agaataaata
     7021 gagaaaagtt ggagtataat agaatttgtt atgaattaga acatgattcc agaagaaata
     7081 aaatatttac tcaacttaaa aatatgatag atataagaaa gggagagagt gcattttctc
     7141 catatgcaaa tcaaaatgta ttggattttg gaagagagat atttgcacta gaacgtgaga
     7201 ataaagaaac taatgagaag gtagttttta taactaatgt tacgcctaat gagaagatta
     7261 tagactgtgg ttttaatggt ataaatatat tagaaaatac taatattgaa ggaaagttaa
     7321 ctttaggtgc atataaattt gcttggataa aaatcaaata ggagtttctg ccttcagata
     7381 ttacctaagg tggttaaagc ggagtagtat ggtatttatt taaatatgag gtcattgatt
     7441 atggtttagc ccaaaatgtt acatttaagt tctgaaaagt aatatttatt gtccaaaaag
     7501 tagtatttaa agtctaaata gccataaata tatggcaaaa agatgtaaat tgaagtaata
     7561 taaaagtaag ctcaagtata ccaatgacaa gtataccaat gaaaggatgt ttagactttg
     7621 attaatatag ggatatgtga tgatgaatta cattatagat taaagataaa agatatatta
     7681 aaaaaggtat taagctcata taccatggat tataatatac atgaattttc atcaggaaaa
     7741 gagttgttat gtaactatcc aaaaaattta gacatattaa ttattgatat acaaatggag
     7801 atattaaatg gtatagatac ctcaagaaaa atacgagagt ttgacgaaaa tctggagata
     7861 atatttatga cttcatttcc tgaatttatg caagaagggt atgaagtcaa agcatataga
     7921 tacatattga aaccaataaa tgaaaaaaaa atcacaaaaa atattttacc ttgtatagat
     7981 gagataatga aaaaaaggaa taattatttg actatcaaag ttaagaacta tgtagataga
     8041 attaaaatag attctattat ttatatagaa acagatagac ctaatatagt gatttataca
     8101 catgataata catacactac aaaaatgagt atttctaaaa tagaaaaaat attaagtgaa
     8161 tttggatttt tcagatgcca taatagctat ataattaatt taaaattagt acaatctatg
     8221 aatggaaata gtgtagtaat aaatggaaag agtataccag taagtaaata tagagtgaaa
     8281 gggttaaaat tagcaataac taatatatta ggagatataa tatgttagaa acattaaata
     8341 taaatctttg ggatattatt aatataatca caggctgtat agattggatt atattctttt
     8401 tagtcatgag tatattgggg aaaaaaaata tatctttcag aaaatataca atttatatga
     8461 cacttgtact cgtatctatg tcattggtca attttatgag tatatttcca aattttaaga
     8521 ttatcatatg tatagtaata ggttctttat tttatattgc ttcatacaaa gagagtatat
     8581 ataaatgtat attaattacc ttattatttt ggttaggatt aatgatgggg gaaggtttag
     8641 ctataggtat agttgtagtt ataaatcaat taaataatat tgatataata ctaaatggaa
     8701 atttatttag gttagaatcc attgtgatat ctaaaatatt cttatttata atatttatgt
     8761 caattaagta ttttaaatta tctatagaat ttaaacctaa ggatatggtg cttataggtt
     8821 tacctatatt atcaaatata gtaagtttgt tattaatatt tgggtataat cttaagaata
     8881 attttgtaag cgatgataat atatttgtaa ttgtactttc aactttattg attgtgtcat
     8941 ctagcataat aattttaata gtaataggga aaattgtaca agatgataaa gtcaagttag
     9001 aatatgaact gataaatgag agaataaaaa tagattataa aagttatgag tctataaatg
     9061 aaatacatga taaattaaaa tatgtttatc atgatttaaa aaatcatatg atttgtataa
     9121 aaaactatga tactaaagaa gaaattatat cttatgtaaa taatttagaa aataaaataa
     9181 atgattttga gtattttaga aatacaggaa ataaaacatt agatataatt ttaggagaaa
     9241 agatatatct ttgtaaaaaa tataatattg agtttgaaga cagtataaat atatcaaaat
     9301 tacaatttat tcaagatgtt gatatatgtg caatatttgc aaatgcacta gataatgcaa
     9361 ttgaagcatg tatgaaaatg aataataata cagaaaaaag aatagaggtt aaagctacat
     9421 acgtaaatgg atttgccata ataaagttca taaatactaa agtaaatgat attaagttta
     9481 ttgatgaacg tattaagacc agtaaggata ataatagggt acatggaata ggtcttgcaa
     9541 gtatcaaata tatggtaagc aaatatgatg gagaacttat agttaattac tcagataatg
     9601 agtttatatt aaaaataatg ataccaataa aagcttagga ggaagtacaa atgaaaaaaa
     9661 tagcattaag tttacttaaa agtatgtcaa cattaagcct aggaatatct agattatcag
     9721 caaatactgc aagctcatgg atagctcatc aagcagaaga gccacaggct ttaaaaaaat
     9781 tgaaaaaaaa taatacaagt acaaaattct ctctctaatc aagagagagt ttttgtattt
     9841 aaggaggaaa tataaatatg tttaaaagtt tatcatataa atttgcaaat attttggtta
     9901 gaaatgaagt tatagaagat gaagattttg aaatatatag atatggattt gagacattag
     9961 tttattttat cattaatatt tcagtagcat tacttatagg tattgctctt aataaattta
    10021 tacaaacaat tatattttta gtctgttact gtactttgag gcaatttaca ggtggatatc
    10081 atgctagaaa ttatacagaa tgtacaataa catttgcact aatttatata tcaattattt
    10141 tagttactaa gaatatagac atttataagt ttaaatatat attaatacta ttacttttac
    10201 taagtacagt tataatacat aaggttgcgc ccttagagca tagaaataaa cctcttagta
    10261 tctatgagaa gaagaattat agaaatatta taaaaaagat aacattatca ataattatag
    10321 tagttacaat aagtttaata tttaatatta tgagtgaata cattatatat tcctcattag
    10381 ctgtattttt aattacaata ttactattag tacaaattat tattaatttc ttcaaaaaat
    10441 aatctattta gctaagctta atgtccagtt agacatagta tattgaatct aaaacttgag
    10501 ggtgatatta taatagcaag ttagatatta aaaaagtaat tttattagat atttgtagtt
    10561 ttgatatatt tattgatatt ttctgattag aaatatatta gata
//
LOCUS       DAEPXF010000137        10529 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain HB27
            SAMN13376389-rid12479423.denovo.144, whole genome shotgun sequence.
ACCESSION   DAEPXF010000137 DAEPXF010000000
VERSION     DAEPXF010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13376389
            Sequence Read Archive: SRR10518432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10529)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10529)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001069624.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001069624.1
            Genome Coverage       :: 315x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/15/2021 01:54:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,165
            CDSs (total)                      :: 4,076
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 89
            rRNAs                             :: 10, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 10 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 19 of 69
            Pseudo Genes (incomplete)         :: 44 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 10 of 69
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10529
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13376389-rid12479423.denovo.144"
                     /strain="HB27"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="China: Hebei"
                     /collection_date="2017"
     gene            complement(<1..1087)
                     /locus_tag="KRL67_002864"
     CDS             complement(<1..1087)
                     /locus_tag="KRL67_002864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HBH1523730.1"
                     /translation="MNFRLPKKRLPKKIIFISIILISIILFIKYDQNGLREREIINRG
                     KYLSESLVADKEYDEAKKVVKTSFESIPEKKYNKVYNEVWDMLKTISYVPDGTKIVID
                     SLEKMRESDVKLSDECRFNIEKRLASVYIMDNNYSKAVDLTVKSIKLAEKLGNNYEKA
                     RLDVDLASILLKVGSYETAEKLIKDSFKIDIDEGEKNGMVRLYAIINLAEIYVEVGEY
                     DKAIEISSRIKDYKKFVNTDDYNDFEIMASIMQSNAYIGKNNIQKAKTFLEKADYLIK
                     SDRTVHILDKDMYYYMAMGNLEYKSKNYNSAIDNYKKSLEISTKRKLIQEKIKNLNKI
                     LNIYEKQGNVKSLNEYQKILINEYKENK"
     gene            complement(1313..2551)
                     /gene="fabF"
                     /locus_tag="KRL67_002865"
     CDS             complement(1313..2551)
                     /gene="fabF"
                     /locus_tag="KRL67_002865"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HBH1523731.1"
                     /translation="MNKRVVITGLGCVTPLGTGKDEFWSNIKSGVSGIDKITNFDAST
                     YQTQIAGEVKNFHPEEYISKKELKRLDKFAQFAIVSAKLAVEDANLDLDKTDRERVGV
                     IIGSGIGGVEAIETQHKILLEKGNKRVSSLFVPMMIGNMAAGQVSIFLGAKGPNTNVC
                     TACASGTHSIGDAFKVIQRGDADIMVAGGSEAAVTGLAFAGFCNMKAMSTRNDDPKTA
                     SRPFDKDRDGFVMGEGAGIVILEDLEHALARGAKIYAEVVGYGLTADAYHMTTPAENG
                     EGAARSMNMALKDGNVPLEEVDYINAHGTSTYYNDLYETMAIKTVFGEKAYDLCVSST
                     KSMTGHLLGASGAIEAVVCAMSIEDSFVPPTINIQEVGEDLDLDYVPNQGKEKNIRYA
                     LSNSLGFGGHNATIVLKKYV"
     gene            complement(2580..2804)
                     /gene="acpP"
                     /locus_tag="KRL67_002866"
     CDS             complement(2580..2804)
                     /gene="acpP"
                     /locus_tag="KRL67_002866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HBH1523732.1"
                     /translation="MFNKVVEIIIEQLGVEDKEITMETSLMKDLEADSLDAVEIIMAL
                     EDEFGIEIPDTEAENFKSIGDIVKYIEANK"
     gene            complement(2858..3607)
                     /gene="fabG"
                     /locus_tag="KRL67_002867"
     CDS             complement(2858..3607)
                     /gene="fabG"
                     /locus_tag="KRL67_002867"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HBH1523733.1"
                     /translation="MINLTGQVAVVTGGSRGIGKEIAKKLASFGADVVINYTSKEDEA
                     LKTKNEIESMGVKCTSIKCDVSKFDEVNQMIDSVVSEFGKIDILVNNAGITKDGLLMR
                     MKEEDFDRVIDINLKGVFNCTKAVTKPMMKKKYGRIINMTSVVGIMGNAGQTNYCASK
                     AGVIGFTKASARELASRNININAVAPGFIETDMTKVLSDDVKESTLANIPKKSYGKPE
                     DVANAVAFLVSDMSSYITGQVINVDGGMVMQ"
     gene            complement(3607..4557)
                     /gene="fabD"
                     /locus_tag="KRL67_002868"
     CDS             complement(3607..4557)
                     /gene="fabD"
                     /locus_tag="KRL67_002868"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HBH1523734.1"
                     /translation="MGKVALVFPGQGAQYVGMAKDLYENNAVAKSVIDEASDALNMDL
                     KNLMFNGNEEELSKTENTQPAMVTHSVAVLKAVQAQIDLKYNACLGLSLGEYSALVAA
                     NAIDFKDAVCLVKKRGKFMQETVPTGVGAMAAILGLDRNVLENVVKDIQGGIVEVANY
                     NSPGQIVISGENEKIEEAMVKCKEAGAKRAVKLNVSGPFHSSMLKEAGVKLASELEKV
                     NITKPKVDVVANVNADYYKNEGKDLLIKQVSSSVLWEDSIERLLNDGYDTFIEMGPGK
                     TLKAFIKKIASNKKANVNIYNIDGIDSLNEFVQNYRNGEI"
     gene            complement(4573..5502)
                     /gene="fabK"
                     /locus_tag="KRL67_002869"
     CDS             complement(4573..5502)
                     /gene="fabK"
                     /locus_tag="KRL67_002869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-[acyl-carrier-protein] reductase FabK"
                     /protein_id="HBH1523735.1"
                     /translation="MNKICKILNIKYPVIQGGMAWVATASLASAVSNAGGLGIIAAGN
                     APKEAIKKEIVECKKLTDKPFGVNVMLMSPFVDDIIDLIIEEKVQVITTGAGNPAKYM
                     DRLKEAGTKVIPVVPTIALAQRMEKLGATAVIAEGTEGGGHIGELTTMVLVPQVADAV
                     NIPVIAAGGIVDGRGIAASFALGASAVQVGTRFICSEECSVHSNYKNLVLKAKDRDAI
                     VTGRSTGHPVRTLKNKLSKEFLKMEQNGATPEELDKKGTGALRFATVDGDIEKGSFMA
                     GQSAAMVKEITPCKEIIEAMVNQAREIMPAIEL"
     gene            complement(5611..6597)
                     /locus_tag="KRL67_002870"
     CDS             complement(5611..6597)
                     /locus_tag="KRL67_002870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III"
                     /protein_id="HBH1523736.1"
                     /translation="MNTKAGILGVGSYLPEQSYDNFHFEKIMDTSDEWISTRTGIKER
                     RFAKESEATSDLASKAALKAIECAKLNVEDIELIILATITPDMSLPSTACIVQDAIGA
                     VNATAFDISAACSGFVYGVTIAKQFVETGCYKNVLVIGAETCSKFLNYDDRTTAVLFG
                     DGAGAAVIGPVSEGGILSTHMGSDGKGKDCLKVPAGGSRLKASKETVEANLHTIEMAG
                     SDVFKFAVRKMAETSLRALEKANLNTTDIDYLVPHQANIRIIQASSKRLELDMKKVYV
                     NIDKYGNMSAASIPVALDEAYREGKIKKGDNVVLVGFGGGLTWGASVVKWTL"
     gene            complement(6623..7645)
                     /gene="plsX"
                     /locus_tag="KRL67_002871"
     CDS             complement(6623..7645)
                     /gene="plsX"
                     /locus_tag="KRL67_002871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HBH1523737.1"
                     /translation="MKIVIDGMGGDNAPKSNVEGAVNAIKEYQVDLIITGDKDLLEKE
                     FSNYEFDRNKLEIVHTTEIIENEDKPVKAIRSKKDSSMVVALNLVKEGKADAIISAGN
                     TGALLAGGLFVVGRIKGIDRPCLCSAIPNVKRGMTLIADCGANADCKPKNLVEFAAMS
                     NIYSRKVLGLENPKVALANVGLEEGKGNDLVKRSYEEIKKLDLNFIGNVEAREVINAY
                     TDIIICDGFTGNILLKSAEGVALSVMSLIKETFMASTKSKIGALLIKDDLRKLKSFID
                     YSEYGGAPLLGLNGGVIKAHGSSDAKAIKNAINQGIKFSKGKVVEDINQFISKYNEEN
                     KNNEDE"
     gene            complement(7656..8213)
                     /gene="fapR"
                     /locus_tag="KRL67_002872"
     CDS             complement(7656..8213)
                     /gene="fapR"
                     /locus_tag="KRL67_002872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription factor FapR"
                     /protein_id="HBH1523738.1"
                     /translation="MKKKSKAQRQKELIDMLKTDPFYTDEELSSLFDVSIQTIRLDRM
                     SLNIPELRERVKSIAETQSSKVKTLGVKEITGEIIDLSVGRLGISMLEVTQDMIYSKT
                     NTLKDTYIFSLADSLAMAIIDAPKVIMRVANVKSFKLIEQQDRLIAKAEVYRNIDKKH
                     YVKVVINNKAQEQIFRGKFIFEELD"
     gene            complement(8420..8596)
                     /gene="rpmF"
                     /locus_tag="KRL67_002873"
     CDS             complement(8420..8596)
                     /gene="rpmF"
                     /locus_tag="KRL67_002873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HBH1523739.1"
                     /translation="MAVPKRKTSKSNTKMRRAANSKMEATGFVSCPQCHEPKLPHRVC
                     PDCGYYKGKEVVSK"
     gene            complement(8621..9145)
                     /locus_tag="KRL67_002874"
     CDS             complement(8621..9145)
                     /locus_tag="KRL67_002874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HBH1523740.1"
                     /translation="MKVSIEKINRKETDKIDLNFCEKIDTISYCDEIYKLVSPVNLKG
                     KVSKTNKGLYLDIDVNFTIVDNCSRCLKEVEIPLEYSIQGFLVKEEDYDEDEFEEFDP
                     FIFDGEEIDLIDIIEQTLDFNVPHKVLCSENCKGLCQVCGANLNEEECSCSEITNDEE
                     YIDPRFAKLKDLFN"
     gene            complement(9238..10434)
                     /locus_tag="KRL67_002875"
     CDS             complement(9238..10434)
                     /locus_tag="KRL67_002875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HBH1523741.1"
                     /translation="MKILVLNCGSSSLKYQLIDMNNEEVLCIGLVERIGIEGSILKHE
                     KAGRDDKYVVEQPMKDHKDAIALVLEAVAHPEFGAVKEMKEIDAVGHRVVHAGEKFAT
                     SVVITPEVEEALKECIDLAPLHNPANIMGIDACKAILPDVPMVGVFDTAFHQTMPKSS
                     YLYGLPHELYTKYGVRRYGFHGTSHNYVSQRAAEILGKDIKDLKIVTCHLGNGASIAA
                     VDGGKCVDTSMGFTPLEGLIMGTRCGDIDPAILPFLMRKEGLDADGLDKLMNKESGVY
                     GMTGISSDFRDIEDAAKNGDERAQATLEAYVKKVQKYIGAYAAEMNGLDVVVFTAGVG
                     ENGKAIRADIASNMEFLGMKLDKEANDVRGKETVISTADSKVKMLLIPTNEELMIARD
                     TLRLVK"
BASE COUNT         3050 a         2151 c         1137 g         4191 t
ORIGIN      
        1 ttttgttctc tttatattca tttattaata tcttttgata ctcatttaaa gattttacat
       61 ttccttgttt ttcatatata tttaatatct tatttaaatt ttttattttc tcctgaatta
      121 actttctttt ggtagatatc tctaaacttt ttttatagtt atctattgca gaattataat
      181 ttttgctttt atattctaag tttcccattg ccatataata gtacatatct ttatctaata
      241 tatgaactgt tctatctgat tttataagat aatcagcttt ttctaaaaaa gtctttgcct
      301 tttgtatatt attttttcct atatacgcat ttgactgcat tatagaggcc attatctcaa
      361 aatcattgta atcgtctgta tttacaaact ttttataatc ttttatcctg ctacttattt
      421 caatggcttt atcatattct cccacctcta catatatttc tgctagattt ataatagcat
      481 ataatctaac cattccattt ttttcacctt cgtcaatatc tatcttaaaa gaatctttta
      541 ttaatttctc tgctgtttca tagctaccta cctttaaaag tatgctcgcc aaatcaacat
      601 ctagtcttgc tttttcataa ttattcccca atttctcagc caattttata gactttacag
      661 ttaaatctac tgctttagaa taattattat ccattatata cactgatgct aatctttttt
      721 ctatattaaa tctacactca tcagataact ttacatctga ttctctcatc ttttctaaag
      781 aatcaatgac tatcttcgtt ccatctggaa catacgaaat agtctttaac atatcccaaa
      841 cttcattata tactttattg tatttttttt ctgggataga ttcaaaagaa gtcttaacca
      901 ctttttttgc ttcatcatac tctttatctg ccacaagact ttcagacaga tattttcccc
      961 tatttataat ttctctttct ctcaatccat tttggtcata ctttataaac aaaataatac
     1021 taataagtat tatacttata aaaattatct ttttgggtaa ccttttcttt ggtagtctaa
     1081 aattcaaaat tttagtcctc cttttgtata ttgtagaatg aaaacttgca tatataaata
     1141 acctcttaat aattaataat acaatatatc aaaatgattt acaacatatg ctagacttaa
     1201 atcttaataa aaaagtctcc taaaataatt taggagactt taaatttaag ccaatataaa
     1261 tttatacagt taaaacgagt acttgttttt taattttata tttaatattt atttatacat
     1321 atttttttag tactattgta gcattatgcc caccaaatcc taaagaattt gatagtgcat
     1381 atctaatatt tttttcttta ccttgatttg gaacataatc taaatccaaa tcttcaccaa
     1441 cttcttgtat gtttatagtt ggaggcacaa aactatcttc tatactcata gcacaaacaa
     1501 cagcctctat agcacctgat gctcctaata agtgaccagt catagatttt gttgatgaca
     1561 cacataagtc atatgccttt tctccaaata cagtttttat agccatagtt tcatataaat
     1621 cattgtaata agttgatgtt ccatgagcat ttatataatc tacttcttct aatggaacat
     1681 ttccatcttt caaagccata ttcatagacc tagctgctcc ctctccattt tcagctggag
     1741 ttgtcatatg gtatgcatca gcagttaaac catatccaac cacttctgca tatattttag
     1801 cccctcttgc taaagcatgt tctaagtctt ctaagataac gatacctgct ccttctccca
     1861 ttacaaaacc atctctgtct ttatcaaatg gtcttgatgc agttttaggg tcatcatttc
     1921 tagttgacat agccttcatg ttacagaatc cagcaaaagc aagacctgtt acagcagctt
     1981 cagagccacc tgctaccata atgtctgcat cacctctttg aattacctta aaagcatcac
     2041 ctattgaatg agttcctgat gcacaagctg tacatacatt agtattaggc ccttttgctc
     2101 ctaaaaatat tgaaacttga ccagctgcca tatttcctat catcataggc acaaaaagtg
     2161 aacttactct tttatttcct ttttccaaaa gaattttatg ttgagtttct atagcttcaa
     2221 ctccacctat tccagaacct attataacac caacacgctc tctatctgtt ttatctaaat
     2281 caagatttgc atcttcaact gctaattttg cagacactat tgcaaattga gcaaacttat
     2341 ctaatctctt caattctttt ttgcttatat attcttctgg atgaaaattc ttaacttctc
     2401 ctgctatctg agtttgatac gtgctagcat cgaaattagt tattttatct ataccagata
     2461 ctccagattt tatattgctc cagaactcat cttttcccgt tcccaacggc gtaacacatc
     2521 caagccctgt tattactact cttttattca tcggatacct ccactttttt aaagctcgac
     2581 tatttattag cttctatata cttaactata tctcctatag atttgaagtt ttctgcttct
     2641 gtatctggta tttctatacc aaattcatct tcaagtgcca ttattatttc tactgcatct
     2701 aatgaatctg cttctaaatc cttcattaat gatgtctcca tagttatttc tttatcttct
     2761 actcctaatt gctctattat tatttctaca actttattaa acattgtgta cctcctaaaa
     2821 ttttttattt taatagattt ctctactttt taaattatta ttgcattacc attccaccat
     2881 ctacatttat tacttgtcct gttatataac ttgacatgtc actaactaaa aatgctacgg
     2941 cattggctac atcttctggt ttaccataag atttctttgg tatatttgct agtgttgact
     3001 cttttacatc atcacttagt actttagtca tatctgtttc tataaatcca ggtgctactg
     3061 catttatatt tatgtttctt gatgctaact ctcttgcaga agcttttgta aatccaatta
     3121 cacctgcttt tgatgcgcaa taattagttt gccctgcatt acccataatt ccaactactg
     3181 aagtcatatt tattattctt ccatacttct ttttcatcat aggtttggta actgcttttg
     3241 tacaattaaa gacacctttt aagtttatat ctataactct atcaaaatct tcttctttca
     3301 ttctcataag cagaccatct ttagttatgc ctgcattatt aaccaatata tcaatttttc
     3361 caaattcgct tacaacagaa tctatcattt gattcacttc atcaaattta gacacatcac
     3421 attttataga ggtacacttt acccccatac tttctatttc atttttagtt tttagtgctt
     3481 catcttcttt agaagtataa ttgattacta catcagctcc aaaagatgct agtttttttg
     3541 ctatttcttt tcctataccc ctagagccac cagtaacaac tgctacttgt cctgtaagat
     3601 ttatcattat atctctccat ttctataatt ttgtacaaat tcattaagag aatctatacc
     3661 atctatatta taaatattga catttgcttt tttattggaa gctatcttct taataaatgc
     3721 ttttaaagtt tttccaggtc ccatttctat aaaagtatca tatccatcat ttaaaagtct
     3781 ttctatagaa tcttcccaaa gaacagatga acttacttgt tttataagca ggtctttacc
     3841 ttcgttttta tagtaatctg catttacatt tgccaccaca tcaactttag gtttagttat
     3901 gttaactttt tctaattcac tagccaattt tacaccagct tcttttaaca ttgatgagtg
     3961 aaaaggtcca cttacattta atttaactgc cctttttgct cctgcttctt tgcattttac
     4021 cattgcttct tctatctttt cattttcacc agatataact atttgtccag gagaattgta
     4081 atttgctact tctacaatac ctccttgtat atctttaacc acattttcaa gtacgtttct
     4141 atctaaacct aaaattgctg ccatagctcc aacacctgtt ggaactgttt cctgcataaa
     4201 cttacctctt tttttaacta aacatacagc atctttaaag tcaattgcat ttgctgcaac
     4261 caacgcacta tactctccta aggataatcc taagcatgca ttatatttta aatctatttg
     4321 agcttgtact gcttttaata ctgctacact atgtgtaacc atagcaggtt gagtgttttc
     4381 agttttagaa agttcttctt catttccatt aaacataagg ttttttaaat ccatgtttaa
     4441 ggcatcacta gcctcatcta ttacactttt agcaacagca ttattttcat ataaatcctt
     4501 ggccatacct acatattgag ctccttgacc agggaatact agtgcaactt ttcccatctt
     4561 actcctcctg atttacagtt ctattgctgg cataatctct cttgcttgat ttaccatagc
     4621 ctctataatt tccttacaag gtgttatttc ttttaccata gcagcacttt gacctgccat
     4681 aaatgaacct ttttctatgt ctccatctac tgttgcaaat cttaaagctc ctgtaccttt
     4741 tttatccagt tcttcaggag tagctccatt ttgttccatc tttaaaaatt cttttgataa
     4801 tttatttttt aatgttctta ctggatgacc agtacttctt cctgttacaa ttgcatctct
     4861 atcttttgct tttagtacta agtttttata gtttgaatgg acagagcact cttcactgca
     4921 aataaatcta gtgcctactt gaactgcact ggcacctaat gcaaatgatg ccgcaattcc
     4981 tctaccatct acaattcctc cagcagctat tacaggtatg tttacagcat cagcaacttg
     5041 tggaactaag accatagtag taagttctcc tatatgtcct ccaccttcag taccctctgc
     5101 tattactgct gtagctccta gcttttccat tctttgcgcc aaagctattg taggtactac
     5161 aggaataacc tttgttccag cttcctttaa tctatccata tactttgcag gatttccagc
     5221 accagtagta ataacttgaa ctttttcttc tataatcaaa tcaattatat catcaacaaa
     5281 tggcgacata agcattacat ttactccaaa aggtttatct gttaattttt tacactcaac
     5341 aatttctttc tttatagctt cttttggtgc gtttcctgct gctattatac caagccctcc
     5401 tgcattagat acagcacttg ctaatgatgc agtagctacc catgccattc ccccttggat
     5461 aactgggtat tttatattta atattttgca aattttattc atacaattct ccttcacttg
     5521 aaatcataaa agtaaacttt ttattattaa tcaactttat tttgtgtata agtttaattt
     5581 tgttaattaa ggcattttag atattttata ctataaagtc cacttgacaa ctgaagcccc
     5641 ccaagttaga cctccaccaa agccaactaa aactacatta tcaccctttt taattttacc
     5701 ttctctataa gcttcatcta atgctactgg tatagatgca gcagacatat ttccatattt
     5761 gtctatattt acataaactt ttttcatatc aagctctaat ctcttagagg aagcttgtat
     5821 gattcttata ttagcttgat gtggcactaa gtagtctata tcagtcgtat ttagatttgc
     5881 tttctcaaga gctcttaaag aagtttctgc catctttcta actgcaaatt taaacacatc
     5941 actacctgcc atttctattg tatgtagatt agcttctaca gtctctttac ttgccttaag
     6001 tcttgaacct ccagcaggta cttttaaaca atcttttcct ttaccatcag agcccatatg
     6061 tgtagacagt attccacctt cacttacagg accaataaca gctgctccag ctccatctcc
     6121 aaacaatact gctgttgttc tatcatcata atttaaaaac ttagaacaag tttctgctcc
     6181 tattacaagc acatttttat agcaacctgt ttccacaaat tgctttgcaa tagttactcc
     6241 atatacaaat ccagaacaag ctgctgatat atcaaatgca gtagcattca ctgctcctat
     6301 tgcatcttgt actatacatg cagttgaagg caaagacata tcaggagtaa ttgtagctaa
     6361 tataatcagc tctatatcct ctacgtttaa ctttgcacat tctatagctt ttaaggcagc
     6421 tttagatgct aagtcagaag ttgcttcaga ctctttggca aatcttcttt ccttaatacc
     6481 agttctagta cttatccact catctgatgt atccattatt ttttcgaagt gaaaattatc
     6541 atatgattgc tcaggtagat aacttcctac tcctaatatt ccagcctttg tattcattat
     6601 atattaaacc tccaaaacga gtttattcat cttcgttatt tttattttct tcattgtatt
     6661 tgcttataaa ttggttaata tcttcaacaa cttttccctt tgaaaatttt attccttgat
     6721 ttatagcatt ttttatagct ttagcatcag aactaccatg agcttttata actcctccat
     6781 ttagacctaa aagtggagct cccccatact cagaataatc tataaaactt tttaattttc
     6841 ttaaatcatc ctttataagt aatgctccta ttttactttt tgtactggcc ataaaagttt
     6901 cttttataag actcataact gaaagtgcta caccttcagc agatttaagt aatatattac
     6961 ctgtaaatcc atcacatatg attatatctg tgtatgcatt tataacttct ctagcttcta
     7021 cattccctat aaaatttaaa tctaattttt taatttcttc atatgacctt ttgactaaat
     7081 cattgccttt gccttcttct agtccaacat ttgctaatgc taccttagga ttttcaagac
     7141 ctaaaacttt tcttgaataa atattactca tagctgcaaa ttcaactaga ttcttaggtt
     7201 tacaatctgc atttgcgcca cagtctgcta taagtgtcat tcctcgcttt acatttggta
     7261 ttgctgaaca taagcaaggt ctatcgattc cttttattct accaacaaca aataatccac
     7321 cagcaagtaa ggctcctgta tttccagctg atattatagc atctgccttt ccttctttta
     7381 caagatttaa tgctactacc atagatgaat cttttttact tcttatagcc tttactggtt
     7441 tatcttcgtt ttctataatc tcagtagtat gaactatctc taatttattt ctatcaaatt
     7501 cataatttga aaattccttt tcaagtaaat ctttatctcc tgtgattatt aaatcaacct
     7561 gatattcttt tatagcattt actgcgcctt ctacattaga tttaggtgca ttatctccac
     7621 ccattccatc aattactatt ttcataattt ccctcctaat ctaattcttc aaagataaac
     7681 ttccctctaa atatttgctc ttgggcttta ttgttgataa caacttttac ataatgcttt
     7741 ttatctatat ttctatacac ttctgctttt gctataagcc tatcttgttg ttctatcaac
     7801 ttaaaacttt ttacatttgc aactctcata ataaccttag gtgcatctat tatagccatt
     7861 gccaatgaat ctgctagaga aaatatatac gtatctttta aagtattagt cttagagtaa
     7921 atcatgtctt gtgtaacttc taacatagat atacctaatc gccctacaga taaatcaatg
     7981 atttctcctg taatttcctt tactcctaaa gtctttactt ttgaactctg agtttctgct
     8041 attgatttga ctctttctct tagctctggt atgtttagac tcattctatc taatcttatg
     8101 gtctgtatac ttacatcaaa cagacttgat aattcttcat cagtataaaa aggatctgtc
     8161 tttagcatat ctataagctc tttttgtctt tgggctttac ttttcttttt catgttccct
     8221 ccaaatttta tagctgtgat tagtaccagc tactaatagt agtatatagt aacacacaaa
     8281 aattttcaat acttatatta ttatttttct attttttaat atgcataaaa tccacttatt
     8341 ttctctagta aatcatctta tttttttata tgtacaaaaa aaacatgtag agtatactct
     8401 acatgctttt tagaagctat tacttagaca caacttcttt acctttataa tatccacagt
     8461 ctggacacac tctatgtggt aattttggct catgacattg tgggcaactt acaaatcctg
     8521 ttgcttccat ctttgagtta gctgctcttc tcatttttgt gtttgattta gacgttttac
     8581 gctttggtac tgccatttac gccacctcct tagtcaaaat ttaattaaat aaatctttta
     8641 atttagcaaa acgaggatct atgtattcct catcgtttgt aatttcactg caagaacact
     8701 cttcttcatt taagtttgcc ccgcatactt gacaaagacc tttacagttt tcactacaaa
     8761 gaactttatg aggtacatta aaatctaatg tctgttctat tatatcaata aggtcgattt
     8821 cttcaccatc gaatataaaa ggatcaaatt cctcaaactc atcttcatca taatcttctt
     8881 cttttactaa aaaaccttgt atagaatact ctagtggtat ttctacttct ttaagacatc
     8941 ttgaacaatt gtcaacaatt gtaaaattga catctatatc tagatacaaa cctttattag
     9001 tctttgacac tttccctttt aaatttacag gtgatactaa tttgtaaatc tcatcacaat
     9061 aactaatagt atcaattttt tcacaaaaat tcaagtctat tttgtcagtt tcttttctat
     9121 ttattttttc tatgctaact ttcattaagt tcacctcaac atatcaccaa atttattata
     9181 caaatctagc atttgtttgt caagtatttt tacttgatag gcataggttt aatttaatta
     9241 ttttaccaat cttaatgtat ctctagctat cattaattct tcattagttg gtattaaaag
     9301 catttttact ttagagtcag cagtagatat tactgtttct tttcctctaa catcattagc
     9361 ttctttatct aatttcattc ctaaaaactc catgttagaa gcaatatctg ctcttatagc
     9421 ctttccattt tcaccaacgc ctgcagtaaa tactacaaca tccaatccat tcatttcagc
     9481 agcataagca cctatatatt tttgtacttt tttaacatac gcttctaatg tagcttgagc
     9541 tctttcgtct ccatttttag ctgcatcttc tatatctctg aagtcactag aaattccagt
     9601 cattccatat actccagatt ctttattcat taatttatcc aatccatcag catctaaacc
     9661 ttcttttctc attaagaatg gtaatatagc tgggtctata tctccacatc tagttcccat
     9721 tattaatcct tctaatggag tgaatcccat agatgtatct acacattttc caccatcaac
     9781 agcagctata gaagcaccat ttcctaagtg acaagttact atttttaaat cttttatatc
     9841 ttttcctaat atctctgctg ctctttgaga tacataatta tgagaagttc cgtggaatcc
     9901 atatcttctt accccatatt tagtatataa ctcatgaggt aaaccatata agtatgaaga
     9961 tttaggcatt gtttgatgga aagcagtatc aaatacacct accattggta catctggaag
    10021 tatagcttta caagcatcta ttcccattat gtttgctgga ttatgaagtg gagctaaatc
    10081 tatacattct tttaaagctt cttctacttc tggagttatt actactgaag ttgcaaattt
    10141 ttctccagca tgtacaactc tatgtcctac tgcatctatt tctttcattt ctttaactgc
    10201 accaaattct ggatgagcaa cggcttctaa aactaatgct attgcatctt tgtggtcctt
    10261 cattggttgt tcaacaacgt atttatcatc tctacctgct ttttcatgtt ttaatataga
    10321 accttctatt cctattcttt caaccaatcc tatacataaa acttcttcat tattcatatc
    10381 tattaattga tattttaatg aagaactccc acagtttaat actaatattt tcattatcat
    10441 ttcctcctta gattttttta taaataatat gtaataaatt ttgatttgta ataaatgtct
    10501 aaataaaact ctacctttta ttattttgt
//
LOCUS       DAEPXF010000138        10455 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain HB27
            SAMN13376389-rid12479423.denovo.145, whole genome shotgun sequence.
ACCESSION   DAEPXF010000138 DAEPXF010000000
VERSION     DAEPXF010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13376389
            Sequence Read Archive: SRR10518432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10455)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10455)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001069624.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001069624.1
            Genome Coverage       :: 315x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/15/2021 01:54:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,165
            CDSs (total)                      :: 4,076
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 89
            rRNAs                             :: 10, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 10 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 19 of 69
            Pseudo Genes (incomplete)         :: 44 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 10 of 69
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10455
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13376389-rid12479423.denovo.145"
                     /strain="HB27"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="China: Hebei"
                     /collection_date="2017"
     gene            121..738
                     /locus_tag="KRL67_002876"
     CDS             121..738
                     /locus_tag="KRL67_002876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HBH1523742.1"
                     /translation="MEIFAIGHSNYPYDKLIDMIKKYDINCVVDIRETPYSKYNIQYN
                     KEAFNESLRNSGFVYIYMGKEFGAKRTNKDVYTQEGYADFEKVAKEDIFLNGIERLKK
                     GCQMGYRIVLLGAMQEPIRCHRSILVGKVLNKEGFDVKYIMHEGNLAYQEDIEESLLD
                     KYFSDRKQLSIDNLLGSALTREEMIQEGYKLANKEIGYRTEKLGK"
     gene            complement(895..1347)
                     /locus_tag="KRL67_002877"
     CDS             complement(895..1347)
                     /locus_tag="KRL67_002877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staygreen family protein"
                     /protein_id="HBH1523743.1"
                     /translation="MVNLNANNLNVNMVYPISSTAPIRFRRYTVSYLENMENIYLTIA
                     TYFDTLGLGTMNIDKVYGQWAWFTDNIYDLNLFVFVGNYPYQIAKNRYDTFVDILPVA
                     ITSIVNGDRVFLISNPCLLNSKITVRFISSHPTLNRTVDYGNIRDFIF"
     gene            1795..3153
                     /locus_tag="KRL67_002878"
     CDS             1795..3153
                     /locus_tag="KRL67_002878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HBH1523744.1"
                     /translation="MFIRKNVIKLAIPIMIEQTFVMLLGVFNTMMAGHIGEEAVSAVG
                     MVDSINNIFISVFAALSVGATVVVAQQIGKKDNEKVNETIKQALVSGVILSFTITILM
                     WVFRTGVINLFYGSAEELVKSNAKLYIEFTLFTYPFIATQQIANGILRGCGDVKKPMY
                     VTMFMNIVNVTLGYILIYGIDLNPLGINLQTPSYGITGAAMSIAIARIIGCIIIIRAL
                     FKGNEFISMSKIFPFKIDVETQKSIFNIGIPAGVEQLLFNAGKLIVQVFIVTMGTSAI
                     ASNAIGMSIASIINVIGNALALSATTLVGQYIGRGDIKGAKSTLLYLTKFATICLFVV
                     GAIFIPIAPWISSMYTQNASVIDISTQLIRSDCLAMVIWPISFVLSAGLKGAGDTRYT
                     MMTAVIGMWIFRIFTGYVLGVVLEFGVLGIWIGMYTDWLVRGTLYCLRLRGTKWLKHK
                     VA"
     gene            3426..3806
                     /locus_tag="KRL67_002879"
     CDS             3426..3806
                     /locus_tag="KRL67_002879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HBH1523745.1"
                     /translation="MINKIGKITLYVNNQEDAKLFWTEKLNFVVTYEKEMGPNMKWVE
                     VAPSKNEFTTFVLYDKNLMMRQNKEINVSHPSIILSTRDINGTYEQMANNEVEVGELM
                     EMPYGRMFSFKDQDGNDYLVREDK"
     gene            3931..4131
                     /locus_tag="KRL67_002880"
     CDS             3931..4131
                     /locus_tag="KRL67_002880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH1523746.1"
                     /translation="MFKKMAVLKDIATKIGRKKAYELLEMVEGNDAFVAEVKIKKNGI
                     ESKKEEIMLKDNQKIILEYIEG"
     gene            4327..5055
                     /locus_tag="KRL67_002881"
     CDS             4327..5055
                     /locus_tag="KRL67_002881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBH1523747.1"
                     /translation="MLEIKNLSFNVESNNEELGIINDVSLSFERGKLIVITGPNGGGK
                     STIAKLIMGIEKATSGQIILDGEDITNLSITERAKKGIGYAFQQPPRIKGMTVENLLT
                     LAHGKPLSTDVCCQYLTDVGLCSKDYLNREVDNSLSGGEMKRIEIATLFARDLKVSIF
                     DEPEAGIDLWSFGKLNESFKKIHEESNQTIIIISHQERILELADEIIVLQDGSVKSHG
                     TKEAILPEIMCQVNSSCELMKDMN"
     gene            5077..5994
                     /locus_tag="KRL67_002882"
     CDS             5077..5994
                     /locus_tag="KRL67_002882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SufD family Fe-S cluster assembly protein"
                     /protein_id="HBH1523748.1"
                     /translation="MNKIDLNLLEKVAELRGTPLGAYNIRKNGQGVERHSTENVTIIP
                     KTDKPGIDIIVKPNTKGENVHIPVIVTETGVQDMVYNDFEIGENADVVIIAGCGIHNC
                     GCDDSEHNGIHRFFLRKGARLKYVEKHYGEGDGEGKRILNPVTIVNMDEDSYCEMETI
                     QIKGVDSTKREMDANLGKGAKIVVFEKLLTHGNQVAESNMKVILEGENSSAQVVSRTV
                     GQDNSKQKFNPCVIGDSLCSAHVQCDSIIMGNSQISAIPEIAANHPDALLVHEAAIGK
                     IAGDQILKMMTLGLTEEEAEQQILNCFLK"
     gene            6136..7320
                     /locus_tag="KRL67_002883"
     CDS             6136..7320
                     /locus_tag="KRL67_002883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HBH1523749.1"
                     /translation="MKNLLIRNGTIISPTDGIKFEKRDILVLHGKIYAIGLNLKEKIR
                     KEKLLTNKEFEIINADNMYISPGFIDVHTHCYKRKLPTGMDSDSIGIEKGSTVIFDAG
                     TAGPLNYYDFKKKYMDECKTEVYSFLNVTNEGLNILNESTSLDVINFDKVESVVEKNI
                     EKIKGIKVKASKFQSNESGIDIIKKSKQVAKKLNLPLVVYIGEYPSYIDEVMNILGKG
                     DVVTPVYGNSTNGILKESGTLRKSVINAKNRGVLFDVCHGLNQFDFKVFKKAIKQGLE
                     PDLISTDMHIKNMKDVNYSLLNVINKIMELGISLEKCIEKVTEYPTKIFDLHGNKGRI
                     KIGGEGDFTIFTMEECNDIIKDYNNNELVLNKKLRLQYTVKSWMKSSEVYRHGYENTI
                     IN"
     gene            7427..7615
                     /locus_tag="KRL67_002884"
     CDS             7427..7615
                     /locus_tag="KRL67_002884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HBH1523750.1"
                     /translation="MKAFVDKDVCVACGACIGTCPDVFSMSDEGHSVAIEGEIPADLQ
                     DLCKEAEEGCPVSAITVK"
     gene            7765..8415
                     /locus_tag="KRL67_002885"
     CDS             7765..8415
                     /locus_tag="KRL67_002885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HBH1523751.1"
                     /translation="MKKNYEIVLFDLDGTLTDPKEGITKSIQYSLNSFGIKEDLENLD
                     QFIGPPLHDTFKEYYKFEDKKAKEAVEKYREYFADKGIFENKIYENIKEILEMLYKNG
                     KILLVATSKPTVFAETILRYFDIDRYFKYIAGSNLDGTRVNKNEVIQYVLDLCNVKDK
                     DKVIMVGDRKYDIIGAKKIGIDSIGVLYGYGSFEELSESEPTYIVENVESIKDILL"
     gene            8518..9456
                     /locus_tag="KRL67_002886"
     CDS             8518..9456
                     /locus_tag="KRL67_002886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HBH1523752.1"
                     /translation="MKSILIMGGSDFIGSALAKRLIKCGYQIDILTNGKKEIDYNGFK
                     EHLICDRKVRKDMENIITGRKYDYIYDMTAYTKEDVSNLIDFISMDNLKKYIVLSAGA
                     VYKDSGRNIKEENEKGENENWGKYGLNKKEAEDFIINSPIPYIIIRPTYIYGENNNLY
                     REYYFFEKIEKNEKIPVPKGKQVSNQFIYIGDLVKVLESIMKNPHVREAYNVTNPQLI
                     SWDDLIYTCGEIIGKEPIIKYVDMEKVEFRERTYFPFRNIDFNLDINKLIEHGLYIPN
                     VLLKEGLTATYKWFSANKPKMHDRKMNKVESVMQIV"
     gene            9482..10333
                     /locus_tag="KRL67_002887"
     CDS             9482..10333
                     /locus_tag="KRL67_002887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HBH1523753.1"
                     /translation="MNSKIKYFLKFFILIFILIFIYSVAIEPSLLIVKKYNISENGTL
                     IKSDTIESKSERIKVVQISDTQIGSFYSTKNLKKVANKINTLNPDIIVFTGDLIDYSN
                     KNPSVDEITTILSSMNARLGKFSVFGNHDYMYKLPRYYRQIMKNSNFNLLVNENKKIK
                     LKDDKYINILGVDEILNGNPNIKYLESQIDNKNFNLLLAHEPDLVDMLSKDTMNLVLS
                     GHSHGGQIRLPIKGALVTPPYGRKYTKGFYDINGNHLYVSSGLGSTKLPFRFFNIPEI
                     IEFNIII"
BASE COUNT         4250 a          999 c         1851 g         3355 t
ORIGIN      
        1 aaaatataaa gtataaagaa aaaagatatt ggatatttaa agtaatactt tatttaattt
       61 taagtaaatc gggtatctac atatatagat actaatttca aagaatggga gggtatttat
      121 atggaaatat tcgcaatagg acattcaaat tatccatatg ataagcttat tgatatgata
      181 aaaaaatatg acataaattg tgtagttgac attagagaaa ctccatattc taaatataat
      241 attcaataca ataaagaggc atttaacgaa tctcttagaa attccggatt tgtatatata
      301 tacatgggaa aagaatttgg agctaaaaga actaataaag atgtatatac gcaagaaggt
      361 tatgcagatt ttgagaaagt agcaaaagaa gatatttttt taaacggaat agaaagatta
      421 aaaaagggtt gtcaaatggg ttataggata gttttattag gagctatgca agaacctata
      481 agatgccata gaagtatttt ggtgggaaaa gtattaaata aagaaggttt tgatgtaaaa
      541 tatataatgc atgaaggaaa tttggcatat caggaagata tagaggaatc gttattggat
      601 aagtatttta gtgatagaaa gcaactgagt attgacaatt tattagggag tgcattaacg
      661 agagaagaaa tgatacaaga aggttataag ctagctaata aagaaatagg atatagaact
      721 gagaaactag gtaaataaaa atataaaagt agataaaata cagtacttac cttaagtaaa
      781 ataatattat gaatttataa tagataggca tgatgtgcca attaaaaata ttctagttat
      841 ttaataatat cattaaattt agggtatttt ttaatagcac atcatgttgc agttttaaaa
      901 aataaaatct ctaatattgc cataatcaac tgttctatta agagtaggat gactagatat
      961 aaatctaact gtaatttttg aatttaacaa acaaggattt gaaattaaaa atactctatc
     1021 tccattgaca atagaagtaa tggctactgg caaaatatct acaaatgtat catatctgtt
     1081 tttagcaatt tgataaggat aatttcctac aaaaacaaat agattcaaat catatatatt
     1141 atcagtaaac caagcccact ggccataaac tttgtcgata ttcattgtgc ctaaaccaag
     1201 tgtatcaaag tatgttgcta tagttaaata aatattttcc atgttttcta aatatgaaac
     1261 agtatatctt ctgaaacgta tgggtgctgt ggaagaaata ggataaacca tatttacatt
     1321 tagattattt gcatttaaat taaccaaagt atcacatcct aaaattagtc tataaaatac
     1381 aatatttaaa tggatgcgat tttgttacat ctaaagtgta atattttata tattgaaaaa
     1441 gtggtaaaag tttgaatgat taaataatac tataaaaaat ttataaaatg tacttattgt
     1501 tattgctaca cgatagtgat acaatattaa cgaaatatag ctacttttaa taataactga
     1561 aaatatttat attaaattaa taaatatttt aaatgtggta tccataaaaa ataataaaca
     1621 taagataatt ataaagatat actgtgttca agtgatatta aacaagattt atattatttg
     1681 aaagcagtat attataatgc gaacaataca attctaaact ttgcattgtc aatttacagt
     1741 tcgataaact aattaaaaat taaatttata taaagaggga aaagaggaat agggatgttt
     1801 ataagaaaaa atgttataaa gttagcaatt cctataatga tagagcagac gtttgtcatg
     1861 cttttaggag tatttaatac aatgatggca ggtcatattg gggaagaagc tgtatctgca
     1921 gttggaatgg ttgattcaat aaacaatata tttatatctg tatttgctgc attatctgtt
     1981 ggagctactg ttgttgtagc tcaacaaata ggaaaaaaag ataatgaaaa ggtgaatgaa
     2041 actattaagc aggctctagt gtctggagta atattatcct ttacaataac aatattgatg
     2101 tgggttttta gaactggtgt aattaattta ttttatggtt cagcagaaga attggtaaaa
     2161 tcaaatgcaa aattatacat agagtttaca ttatttacgt atccatttat tgcgacacaa
     2221 caaatagcaa atggtatttt aagagggtgt ggagatgtaa agaaacctat gtatgtgact
     2281 atgtttatga atatagtaaa tgtaacatta ggatatatac ttatatatgg tattgattta
     2341 aatcctctag gaataaattt gcaaacacca tcctatggca taacaggtgc agctatgtct
     2401 attgctatag ctagaataat tggatgtata ataattataa gagctttatt taaaggaaac
     2461 gaatttatat ctatgagtaa aatatttcca tttaagattg atgttgaaac tcaaaagtct
     2521 atatttaata tagggatacc agctggagtt gaacaactac tttttaatgc aggtaaattg
     2581 attgttcaag tatttatagt tactatggga acatcagcaa ttgcatccaa tgcaatagga
     2641 atgtcaattg catccataat aaatgtaatt gggaatgctt tagctttgtc tgctacaacc
     2701 ttggtgggac agtatatagg acgtggagat attaaaggag ctaagagtac tttattatat
     2761 ttaactaaat ttgcaacaat ttgtttattt gtagtaggag ctatatttat accaattgca
     2821 ccctggattt caagtatgta tactcaaaat gctagtgtaa ttgacatctc aacacaatta
     2881 attcgaagtg attgtttagc tatggtaata tggcctatat catttgtatt atcagcaggg
     2941 ctaaagggag ctggtgatac tagatataca atgatgactg cagtaatagg tatgtggatt
     3001 tttagaattt ttacaggtta cgtattgggg gttgtattag aatttggagt gttaggaatt
     3061 tggataggta tgtatactga ttggcttgtt agagggactt tatattgttt aagattaaga
     3121 ggaacaaagt ggctaaaaca caaagttgca tagaatatat gaaatcactt ttagatggat
     3181 aactctattt agaagtgatt ttttatgtag ttattatcat aattataagt atgatatata
     3241 aggtgattaa attacataaa taaagcctat aatagtatgg atgctataaa ttatatattg
     3301 agcagaatta cataatttgg cgataaatat tagaaatata tatctataga aaataatata
     3361 tatgggtata ttattaaata taaaaaatat taggtattta tttaataata aaggtgggta
     3421 ttgttatgat aaataaaata ggaaaaataa ctctctatgt aaataatcaa gaggatgcaa
     3481 agttgttttg gacagaaaaa ttgaactttg tagtaactta tgaaaaggaa atgggtccaa
     3541 atatgaaatg ggtggaagta gcaccaagta aaaatgaatt tacaacattt gtgttgtatg
     3601 ataaaaatct aatgatgaga caaaacaaag agataaatgt atctcatcca tcaataattt
     3661 taagtacaag agatattaat ggaacttatg aacaaatggc aaataacgag gttgaggttg
     3721 gggaattaat ggagatgcca tatggaagga tgttttcttt taaagaccaa gatggaaatg
     3781 attatttggt cagagaggat aaataatttt acaaagtatt aaatttactc aatatctgat
     3841 ataattagag acaataatta ttataaacta gtctaggatt agaattatat aattaaaaat
     3901 taagtgtaaa ttttgagtag aggtgataaa atgtttaaga aaatggcagt actaaaagat
     3961 atagcaacta aaataggtcg taaaaaagcg tatgaactat tagaaatggt tgaaggtaat
     4021 gatgcctttg tagctgaggt gaagataaaa aagaatggaa tagaatctaa aaaagaagaa
     4081 attatgttaa aagataatca aaaaataata ttagagtata tagaaggcta atttttaaat
     4141 acatataagt tgatatacta aatttctgag atgagtttac ataaaactca tcttttttac
     4201 ttttcaataa ctttgtttta ttatttaata tacattttat ctgcttagag ttgctgatat
     4261 ataaaaatta ctgatataat aaaagaatgg atattaatgt aatattttat agaaaagagg
     4321 ggtattatgc ttgaaataaa aaatctttcc tttaatgttg agtctaacaa tgaggaacta
     4381 ggaataataa atgatgttag cctttcattt gagaggggaa aacttattgt tatcacaggg
     4441 ccaaatggtg gaggaaaatc tacaattgct aagcttatta tgggaattga aaaagctacg
     4501 agtggtcaga taattttaga tggagaagat ataacaaatc ttagtattac agaaagagca
     4561 aaaaaaggaa taggatatgc atttcaacaa ccaccaagaa taaaaggtat gacagttgaa
     4621 aatttactta cacttgctca tggaaaacca ttgtctactg atgtatgttg ccaatatctt
     4681 acagatgttg gattgtgttc aaaagactat ttaaatcgtg aagttgataa ctctctttct
     4741 ggaggggaaa tgaaaagaat tgagatagca actctttttg ctagagattt aaaggtttct
     4801 atatttgatg aacctgaagc agggatagac ctttggagct ttgggaaact caatgaaagt
     4861 tttaagaaaa tacatgaaga gtctaaccag actataatca taatttctca ccaagaaaga
     4921 atattagagt tagctgatga aataattgtt ctacaagatg gttcagtaaa aagccatgga
     4981 acaaaagagg ctatattacc tgaaatcatg tgtcaagtta attcaagttg tgaattaatg
     5041 aaagacatga attaattaga aaggaaggca aattaaatga acaaaataga tttaaatctt
     5101 ttagaaaaag ttgctgaact tcgtggaact ccattaggag catacaatat acgtaaaaat
     5161 gggcaaggag tagagagaca ttcaactgaa aatgtaacta taattcctaa aactgataaa
     5221 cctggaatag atattatagt aaagccaaat acaaaaggag aaaatgtaca tataccagta
     5281 atagtaacag agactggtgt acaggatatg gtatataatg attttgaaat tggagaaaat
     5341 gcagatgttg ttataattgc tgggtgtggt atacataact gtggttgtga tgatagtgaa
     5401 cataatggta tacatagatt tttcttacgt aaaggagctc gcctaaaata tgtagaaaaa
     5461 cattatggtg aaggtgatgg agaaggaaag agaattttaa atccagttac tattgtcaat
     5521 atggatgagg attcatattg tgaaatggaa acaattcaaa ttaagggtgt agattcaact
     5581 aaaagagaaa tggatgcaaa cctaggtaaa ggagctaaaa tagttgtatt tgagaaatta
     5641 ttaactcatg gtaatcaagt tgcagaatct aatatgaagg ttatcttaga gggggaaaac
     5701 tcttctgctc aagtagtttc tcgtacagtt gggcaagata attctaagca aaagtttaat
     5761 ccttgtgtta ttggggattc cctttgttct gcacatgttc aatgtgattc aataattatg
     5821 ggaaattctc aaattagtgc tatacctgaa attgctgcaa accatccaga tgctctttta
     5881 gtacatgaag cagctattgg aaagattgca ggagaccaaa tactaaagat gatgacatta
     5941 ggtcttactg aagaagaagc cgaacaacaa atacttaatt gcttcttaaa gtaaattaaa
     6001 gattttattt aatttaatat aattaatata gtataatata gtgaataagt ctaaaatatt
     6061 tttattttta ggcttatttt ctttatgtaa gaaaagctat tagagaaatt atattgtaat
     6121 taggatgtgg aataaatgaa aaatttactt ataagaaatg ggacaataat atctccaaca
     6181 gatggtatca aatttgaaaa aagagatatc ttggttttac atgggaaaat atatgctatt
     6241 ggattgaacc ttaaagagaa aataagaaaa gaaaaattac ttacaaataa agagttcgag
     6301 ataataaatg ccgataatat gtatatttcc ccaggattta tagatgttca cacccattgt
     6361 tataagagaa aattacctac aggtatggat tctgattcaa ttggaattga aaaaggttct
     6421 acagttatat ttgatgcagg tacagctggt cctttaaact actatgattt taaaaaaaag
     6481 tatatggatg agtgtaaaac agaagtatat tcttttttaa atgttacaaa tgagggacta
     6541 aatatattga atgaatcaac ttctttagat gttataaatt ttgacaaggt tgagtcagtt
     6601 gtagaaaaaa atatagaaaa gataaagggt ataaaagtaa aagcgagtaa atttcaatct
     6661 aatgaaagtg gtatagatat aattaagaaa tcaaagcaag ttgctaaaaa attaaattta
     6721 cccttagtgg tatatatagg agagtatcca tcatatatag atgaagtaat gaatattttg
     6781 ggaaaaggag atgtggttac accagtatat ggaaatagta caaatgggat ccttaaagaa
     6841 tctggcacat taagaaaatc agtaataaat gcaaagaaca gaggagtatt gtttgatgta
     6901 tgtcatggat tgaaccaatt tgattttaaa gtttttaaaa aagcaataaa gcaaggttta
     6961 gagccagacc taataagtac tgatatgcat ataaaaaaca tgaaagacgt aaactattct
     7021 cttttaaatg ttataaataa aattatggaa cttgggatat ccttagaaaa gtgtatagaa
     7081 aaagtaactg aatacccaac aaagattttc gatttacatg gaaataaagg cagaataaag
     7141 attggtggag aaggtgattt tacaatattc acaatggagg agtgtaatga tattataaaa
     7201 gactacaata ataatgaact tgttttaaat aaaaaattaa gattgcaata tactgtaaaa
     7261 tcatggatga aaagcagtga agtttataga catggatacg aaaatactat aataaactaa
     7321 acaatagtga taaaataata aatatatgtt taaattggta tatattggat aaatatctaa
     7381 ggaaacaaca attaaaaaat aataatttta ggggaggtat tttagtatga aagcatttgt
     7441 tgataaggat gtatgtgtag cttgtggagc atgtatagga acatgtccag acgtttttag
     7501 tatgagcgat gaagggcatt cagtagcaat tgaaggagaa ataccagcag acttacaaga
     7561 tttatgtaaa gaagccgaag aaggatgtcc agtttcagct ataacagtta aatagagctg
     7621 tttaaattta tttttaaaat aaatatataa gatttaattg ggtacttaag ctatagtatt
     7681 agaaatttct attactatag ctttttaaaa tatataaact taaaatataa gatattactt
     7741 tttacaaata tggaggactg aaatatgaaa aaaaattatg aaatagtatt attcgattta
     7801 gatggaacac ttacagaccc taaagaagga ataacaaagt ctattcagta ttctttaaat
     7861 agctttggca taaaagaaga tttggaaaac ttagaccaat ttataggacc accattacat
     7921 gatactttta aggagtatta taaatttgaa gataaaaaag ctaaagaagc tgttgaaaag
     7981 tatagagaat attttgctga taaagggata tttgaaaata aaatctatga gaatataaaa
     8041 gagatattag aaatgctcta taaaaatggt aaaatattat tagtagcaac ctctaagcca
     8101 acagtttttg cagaaactat acttagatat tttgatatag atagatattt taaatatata
     8161 gcaggtagca atcttgatgg tacaagagtg aataaaaatg aagttataca atatgtacta
     8221 gacctatgta atgtaaaaga taaagataaa gttattatgg ttggagatag aaagtatgac
     8281 ataattggag ccaaaaaaat aggtatagat tctattggag tgttgtatgg gtatggttct
     8341 tttgaagaac ttagtgaaag tgaacctact tatattgtcg aaaatgtaga gtctataaaa
     8401 gatattttgt tataaatatc ttttatagac aaaaatatta caaaatggtg tcgcatattt
     8461 aaaaatatga tattatagtt aaggaagtca tttaaaaaag aggaggatgc acttaaaatg
     8521 aaatctattt taattatggg tggaagcgac ttcataggtt ctgcacttgc taagcgcttg
     8581 attaaatgtg ggtatcaaat tgatatactt acaaatggta aaaaggaaat agattataat
     8641 ggttttaaag aacatctaat ttgtgataga aaagttcgaa aagatatgga aaacattatc
     8701 acaggaagaa aatatgatta catatatgat atgacagcat atacaaaaga agatgtaagt
     8761 aatctgattg attttataag tatggataac ttaaaaaagt atatagtatt gtcagcagga
     8821 gcagtatata aagatagtgg aagaaatata aaagaagaaa atgagaaagg tgaaaatgaa
     8881 aactggggaa aatatgggtt aaataaaaaa gaggctgaag attttattat aaatagccct
     8941 ataccatata taattattag accaacttat atatatggtg aaaataataa tttatataga
     9001 gagtattatt tctttgaaaa aatagaaaaa aatgaaaaga taccagtacc aaaaggaaaa
     9061 caagttagta atcaatttat atatattggg gatttagtaa aggtattgga aagcataatg
     9121 aaaaatcctc atgtaagaga ggcctataat gtgacaaatc cacagttaat ttcttgggat
     9181 gaccttatat atacatgtgg agaaattatt ggcaaagaac ctataataaa atatgtagat
     9241 atggaaaaag tagagtttag agaaagaacg tatttcccat ttagaaatat agactttaat
     9301 cttgatataa ataagcttat tgaacatggt ctttatatac caaatgtgtt actaaaagaa
     9361 ggtcttacag caacatataa atggttttca gcaaataagc caaaaatgca tgacagaaaa
     9421 atgaacaaag ttgagtctgt aatgcaaata gtttaaaaaa ataaataagt aggtagatat
     9481 tttgaactca aaaattaagt attttttaaa attttttatc ttaatattta ttttgatttt
     9541 tatatactca gttgcgatag agccgagtct attaattgtg aaaaaatata atataagtga
     9601 aaatggaaca ttgataaaaa gtgatacaat tgagagtaaa tctgaaagga taaaagtagt
     9661 tcaaatttca gatactcaaa taggtagttt ttattcaact aagaatctca aaaaagtagc
     9721 taataagatt aacactttga atccagatat aatagtattt actggtgatt taattgatta
     9781 ctccaataaa aatccaagtg tagatgaaat tacaactatt ttatcaagta tgaatgctag
     9841 gttgggaaaa ttttcagtat ttggcaatca tgattatatg tataaacttc ctaggtatta
     9901 taggcagatt atgaaaaatt ccaacttcaa tcttttagta aatgaaaata aaaaaataaa
     9961 attaaaagat gataaatata ttaatatatt aggtgttgat gaaatattaa atggaaatcc
    10021 taatataaaa tatctggaga gtcaaataga taataagaat tttaatttat tgttagcaca
    10081 tgaaccagat ttagtagata tgcttagtaa agatacaatg aatttagttc tatctgggca
    10141 tagtcatggt ggtcaaataa gattacctat taagggagca ttagttactc ctccatatgg
    10201 aagaaaatat actaaagggt tttatgatat aaatggaaat catttatatg tgagttcggg
    10261 tcttggaagt acaaagttac cgtttagatt ttttaatata ccagagataa tagaatttaa
    10321 tattataatc taacataaaa tatttagaaa atataatttt gctttttata atatgtaaat
    10381 gtatatactg taaaaatggg ttaaaccagt agattgaatg aattaataga ttaagttaat
    10441 atataaaata cttaa
//
LOCUS       DAEPXF010000139        10448 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain HB27
            SAMN13376389-rid12479423.denovo.146, whole genome shotgun sequence.
ACCESSION   DAEPXF010000139 DAEPXF010000000
VERSION     DAEPXF010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13376389
            Sequence Read Archive: SRR10518432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10448)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10448)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001069624.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001069624.1
            Genome Coverage       :: 315x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/15/2021 01:54:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,165
            CDSs (total)                      :: 4,076
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 89
            rRNAs                             :: 10, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 10 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 19 of 69
            Pseudo Genes (incomplete)         :: 44 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 10 of 69
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10448
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13376389-rid12479423.denovo.146"
                     /strain="HB27"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="China: Hebei"
                     /collection_date="2017"
     gene            153..3050
                     /locus_tag="KRL67_002888"
     CDS             153..3050
                     /locus_tag="KRL67_002888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HBH1523754.1"
                     /translation="MKAFKKHFLILSLIFLLIISCFNNILCFADESENSKKIRVGYCD
                     DYGIISSSKEHSYTGYGYDYLREISKYTRWEYEFVKGSWEECLDRLEKGEIDLLGPLQ
                     KNDERNKLFNFPELSSGYEYSALYTKDSNNNMFYEDISSFKGMRVAVLRGNFHNIAFE
                     KYREENNFSVEYIYCNSIGELIESVNEKKADAFVCGSIINAKGMKIVSKFSVEPFYFA
                     TAKDKPNLVKELDYALKELKINDMYYELELYKKYFKREVNNSIAFTRQEMNFIKANPK
                     LTMVYDSEWSPVEYYDKESNSFKGISSNLMSIISKKCGIKFEYIKTKNYNESLDYIKS
                     GKANMLCGSINENDKAKRFNMKLTNPYINIPMIMVGKLDTNLNNDLNIALTSSYKSID
                     SYIEKSFENAKTTSYKSIESCLNAINEGKANLMVLSSYQFDELLREGKSENLSVISVL
                     DTSYGMRIGVSNKTDPILVSILNKSIDKITEEELSDCIYSNTIDKPYKVPFGVIFKEY
                     SIQIISFLCILFLIAIKYIIYNKKKKEDYLKKIAYTDPLTGADSIDKFKINSNKLFAK
                     NNPEEYALFYIDVDKFKYINDMFGYDMGNDTLIHISNTIASELKEDEIFARVSADHFV
                     LLIKYKTDDDIKTRLNNIYNKVQILSNPKINYYKLILDCGIYKISKSDNDINTIMDRA
                     NTARKTIKGGHKNSFAFYDKEMHKKILKEKEIENSMVDALNNGEFIVYFQPKYSLSDY
                     QIIGAEALVRWDNPQKGLIPPIEFIPVFERNGFIVNIDFYVFEEVCKKIREWMDEGQK
                     VVPISVNLSRMHFVNSNFIEKFKLIVDKYKIPTRLIELELTETAVLDNIEGLLDTMNN
                     LKEKGFVISMDDFGTGYSSLNLLKELPVDILKLDRAFFTEKDESNNEKIVISNVIKMA
                     KELKMKVISEGVETISQVEFLKQIGCDMVQGYLFSKPMPVKEFEKIAFKKE"
     gene            3371..3583
                     /locus_tag="KRL67_002889"
     CDS             3371..3583
                     /locus_tag="KRL67_002889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HBH1523755.1"
                     /translation="MMPLILSNTGEEVVIKKISGSDKTRTFLNSIGFVVGTNVKIVSK
                     IDGNLIIDVKDTRVALDKKMASRIII"
     gene            3596..3817
                     /locus_tag="KRL67_002890"
     CDS             3596..3817
                     /locus_tag="KRL67_002890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HBH1523756.1"
                     /translation="MNRLKDIKCGETVKVKKLEGEGATRRRIMDMGITRGVNIFIRKV
                     APLGDPIEVTVRDYELSIRKSDAEKIIVE"
     gene            3917..6073
                     /gene="feoB"
                     /locus_tag="KRL67_002891"
     CDS             3917..6073
                     /gene="feoB"
                     /locus_tag="KRL67_002891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HBH1523757.1"
                     /translation="MSIKIGLIGNPNCGKTTMFNGLTGSSQYVGNWPGVTVEKKGGKL
                     KGNKDVEIVDLPGIYSLSPYTLEEVVTRNFMLDDKPDAVINIVDASNIERNLYLTTQV
                     LELGIPTVIALNMMDIVNKNGDKINIKELSEVIGCPVVEVTAVKGQGIMEAAEKAVEL
                     ASSNNKLNFKLPFVDKSKEAIEKIEKIIEEKTPYIDVETRWLAIKLFERDENVIQKLD
                     ISKAILNSIEEITRNCEDELDDDSESIITANRYEFISSIISNIIKKNRKGKETVSDKI
                     DKIVTNRILALPIFALIMWGVYYIAVSSLGTIATDWTNDVLFGEIIQGNVSNFLASLN
                     VAEWLQGLVVDGLIGGVGAVLGFVPQIMLLFLLLSILEDCGYMSRVAFIMDRIFRKFG
                     LSGKSFIPMLISSGCGVPGVMSTRTIENDRDRKMTIMLTTFIPCGAKIPIIALFAGAL
                     FGGASWVAPSMYFLGIAMIIICGIILKKTSLFAGEPSPFVMELPQYHIPSAKGVLIHM
                     WDRGKAFIIKAGTIIFVACGVIWFLQSFNWSLQMVDAGDSILASLGNIIAPIFAPLGF
                     GNWQSSVATVTGLVAKENVVGTFGVLFGISDATEQDPTLLASVASMFTVASAFAFMAF
                     NMLCAPCFAAIGAIKREMGSWKWTWITLGFQTLTAYIIALLINQVGSLVLGTGGSIAG
                     AIISIVIAVAVVFVVLTYSNKNMKKEKMGKLSYMKN"
     gene            6099..6251
                     /locus_tag="KRL67_002892"
     CDS             6099..6251
                     /locus_tag="KRL67_002892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FeoB-associated Cys-rich membrane protein"
                     /protein_id="HBH1523758.1"
                     /translation="MATFIIAAIVVVLMALAVMHMVKNSKKNGSSCGCGCSGCSSSKS
                     CHSAKR"
     gene            6432..7142
                     /locus_tag="KRL67_002893"
     CDS             6432..7142
                     /locus_tag="KRL67_002893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIM24 family protein"
                     /protein_id="HBH1523759.1"
                     /translation="MFKFDVTKELMCIARGSGKFFAKKGAMVAFKGNFNFEKLLLGPS
                     NGGGLGRALLGHVQRSLTGEQMPIMVVEGEGEIYLAQNAYHVDVISIDPGDSIYVESE
                     NLLAFSEQLNYSVKLIGSGVISQKGLFTTHLVNKTGQNQDVAIITDGNPLILEGPCCV
                     DPDALVAWTGREPYPKVSKLSWKTFIGQTSGESYHMEFVEPGQIVIIQPSERLSGLKT
                     DSTPSDRMSSGGLRLGID"
     gene            7227..7997
                     /locus_tag="KRL67_002894"
     CDS             7227..7997
                     /locus_tag="KRL67_002894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBH1523760.1"
                     /translation="MFEKIHKRFKGYYIFLIILILWKIVCVLGIWSSYILPPPEIVLD
                     TFIKMIYDGSIFLNVYASVRRILIGFLISSLMAIPLGVVFGVNKKIYEYFKPLFEFMR
                     STPPLALVPMLILWFGIGEESKIIIIILASFFPIFLNTLKGIKNCDDKLIEVGKSFNL
                     SRKRIFYKIIIPNSMLDIVVGLSLGLGYSFRAIIGAELIAASSGLGYLISDGKDMSRT
                     DVVLVGIFVIGFLGILSDYLFSKIITKFSKGKQVDLNG"
     gene            7990..8721
                     /locus_tag="KRL67_002895"
     CDS             7990..8721
                     /locus_tag="KRL67_002895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBH1523761.1"
                     /translation="MVRSGFLLENIYKKYLVDNKEHLVLDNISLNISSEEITVILGES
                     GCGKTTLLRILAGLENATNGNVYFFNNDKKCTPKVGMVFQESRLMPWLNVSENILLHT
                     EKDNRNKVDLDKYLKMMKLEKFKNSYPNELSGGMAHRVSIARALSFNPDILLMDEPFA
                     ALDYFTRRKMQKEVVNIHKNTKKGVVFVTHNIEEAMEIAKKIIVFSKNKRIKQFSVED
                     EYNRDLTKNYYINLKKEILRELGEF"
     gene            8755..9714
                     /locus_tag="KRL67_002896"
     CDS             8755..9714
                     /locus_tag="KRL67_002896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NrtA/SsuA/CpmA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HBH1523762.1"
                     /translation="MTSLIMVLVGTVGCSTTNDKKDEPAKKEASLPKEINLTYVKSPL
                     NVPSILEKKDDLFGKEFKKDNIKVNFHELTTGPEQTQALAAGELDFLHALGGTSAIIA
                     ASNGVGLKITNIYSRSPKGYMLITNNENIKSIKDLKGKKIVGPKGTILHQLLIAALAK
                     EGYTIDDVEYVNMDIPSAASALESKSADVAMLAGPVALKTINSGAKMIVNGEGLVSGI
                     IVTAVSDDFAEKYPELVKRFMKVHEETLKYMNENKDEVMDVVSKEVGLSLDETKEMYS
                     WYDFSSKITDKDIKELEDTQEFLMSNGMQQKKINIKDMLYNQN"
     gene            complement(9808..10404)
                     /locus_tag="KRL67_002897"
     CDS             complement(9808..10404)
                     /locus_tag="KRL67_002897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3793 family protein"
                     /protein_id="HBH1523763.1"
                     /translation="MSCCNSQFCCKNKIESSYIKWLLEVLGPVFLGSKPAEIINISFT
                     DVNREEKINDIYKYLYKCKKIDFIVIDKEKKGLKILFVNKKALSEKLKCKKTVNFLKF
                     LGYKQNTNVNAYLEHLVDKLKSDVFPDEIGIFLGYPLKDVIGFMGYSNYEVSMIKYWK
                     VYGDTKQSEDTYSKFLLHRKKMRKLLDYISVDKIVSCF"
BASE COUNT         4027 a         1084 c         1894 g         3443 t
ORIGIN      
        1 tatttaaata gataataata aatttattat agttgaataa gaatattata tgagaatatt
       61 gcaatttgat atatttacca ttacaataaa agtagttaca actagaaaac caaataagtt
      121 aaagtaagtt ggcaaattgg aggtttttaa atatgaaagc atttaaaaag cacttcttga
      181 tactttcttt gatattttta cttattatat catgttttaa caatatacta tgctttgctg
      241 atgagtctga aaattcaaaa aaaataagag ttggatattg tgatgactac ggaataatat
      301 cctcatcaaa agagcattct tatacaggat atggttatga ttatctaaga gaaatctcaa
      361 aatatacaag atgggaatat gaatttgtaa aagggtcttg ggaagaatgt ttagacagac
      421 tagaaaaagg tgaaatagac ttacttggac ctttacagaa gaatgatgaa agaaataaac
      481 tatttaattt tcctgaacta agttcaggat atgaatatag tgcactatat acaaaagata
      541 gtaacaataa tatgttttat gaagatataa gttcctttaa gggaatgaga gtagctgtac
      601 ttagaggaaa ctttcataat attgcatttg agaagtatag agaagaaaat aatttttcag
      661 ttgaatatat atactgtaat tctattggtg aattaattga gtctgtcaat gaaaaaaagg
      721 cagatgcatt tgtctgtgga agtattataa atgctaaagg aatgaaaata gttagcaaat
      781 tttcagtaga gcctttttat tttgctactg caaaagataa acctaatttg gtaaaagaat
      841 tagattatgc tttaaaagaa ttaaaaataa atgatatgta ttatgagttg gaattatata
      901 aaaaatattt caaaagagag gtcaataatt ccatagcatt tactcggcaa gaaatgaatt
      961 ttattaaagc aaacccaaaa cttactatgg tatatgattc agagtggtct cctgtagagt
     1021 attatgataa ggaaagtaat tcgtttaaag gtataagttc caatttaatg tcaattatat
     1081 caaaaaaatg tggtataaaa tttgaatata ttaaaacaaa aaattataat gagagtcttg
     1141 attacataaa aagtggtaag gcaaatatgc tatgtggctc aatcaatgaa aatgataagg
     1201 caaaacgatt taatatgaaa ttgacaaatc catacataaa tattccaatg attatggtag
     1261 gaaaactgga taccaatctc aataatgatt taaatattgc tttaacgagt agttataagt
     1321 ctattgattc ttatatagaa aaaagttttg aaaatgctaa gacaacatct tataagagta
     1381 tagaaagttg tctaaacgct ataaatgaag gtaaagcaaa cttaatggta cttagttctt
     1441 atcagtttga tgaactttta agagaaggaa agagtgagaa tttatctgtt atatctgttt
     1501 tagatacaag ctatgggatg cgtatagggg tatcaaataa aactgaccct atattagtat
     1561 ctattttaaa taaatcaata gacaaaatta ctgaagaaga attaagtgat tgtatatatt
     1621 ctaatactat tgacaaacct tataaagtac catttggtgt gatttttaaa gaatattcaa
     1681 tacaaattat ttctttttta tgtattttat tcttaattgc tattaaatat ataatttata
     1741 ataaaaagaa gaaagaggat tatctaaaga agatagcata tacagaccca ttgacaggag
     1801 cagatagtat agataaattt aaaataaatt cgaataaact gtttgctaaa aataatccag
     1861 aagaatatgc gttgttttat atagatgttg acaaatttaa atatataaat gatatgtttg
     1921 gatacgatat gggaaatgat actttgatac atatatcaaa tactattgca agtgaattaa
     1981 aggaagatga aatttttgca agagtttctg ctgaccattt tgttcttctt attaagtata
     2041 agacagatga tgatattaaa acaaggttaa acaatattta caataaggtt caaatactta
     2101 gtaatcctaa aataaactat tataaattga ttttagattg tggtatatat aaaataagta
     2161 aaagtgataa tgacataaat actattatgg atagagctaa tactgcaaga aaaactataa
     2221 aaggtggtca taaaaattca tttgcttttt acgataagga aatgcataaa aaaatattaa
     2281 aagagaaaga aattgaaaat tcaatggtag atgctcttaa taatggagaa tttattgttt
     2341 attttcaacc taaatatagt ctttcagatt atcaaattat aggggcagag gctcttgttc
     2401 gatgggataa tccacaaaaa ggtctaatac caccaataga atttattcct gtatttgaga
     2461 ggaatggttt tattgtaaat attgatttct atgtatttga ggaagtgtgt aaaaagatta
     2521 gagaatggat ggatgaagga caaaaagtag tacctatatc tgtaaacttg tctcgaatgc
     2581 attttgtaaa tagtaatttt atagagaagt ttaaattaat tgtagataaa tacaaaattc
     2641 cgacaagact tatagaatta gagctaacag aaacagcagt attagataat atagaaggat
     2701 tacttgatac aatgaacaat ttgaaagaaa agggatttgt aatctctatg gatgattttg
     2761 gtacgggata ttcctctctt aatcttttaa aagaattgcc tgttgacata ttaaaactag
     2821 atagagcatt ttttacagaa aaagatgaga gtaataatga aaaaattgtt atatctaatg
     2881 tcattaaaat ggcaaaggaa ctgaaaatga aagtaatatc agaaggtgta gagactattt
     2941 cgcaagtaga atttctaaag caaattgggt gtgacatggt acaaggatat ttgttttcta
     3001 agccaatgcc tgtaaaagag tttgaaaaaa tcgcttttaa aaaagaataa agcaatgtgt
     3061 aagtaatgat atatataata aaggtgcttt aatattagtt gagtatacta ttttagagca
     3121 tctttatttt tgtattaatt tttacgattt tattaataaa acaaatcaaa tgagaaaaaa
     3181 aatcaaaaga atacaataat cacttgcaaa aaatatacta ttgtgataaa cttaaatcaa
     3241 aattaggaat tgtaaaaaga aggaaaaaat aggatattat tttaatagat taagatttaa
     3301 ggtattgtaa tttatatata agataacgat tgcttgtaga tttaaattag tttaagagga
     3361 gggatattat atgatgccat tgatactatc aaatacaggt gaagaagtag ttataaagaa
     3421 aatttcagga agtgataaga cgaggacttt tttaaatagt ataggttttg tagtaggtac
     3481 aaatgttaaa atagtttcta agatagatgg taacttaata atagacgtaa aagatactag
     3541 agttgcatta gataaaaaaa tggcaagtag aataataata taaggaggta atgacatgaa
     3601 cagactgaag gatattaagt gtggagagac tgtaaaagtc aaaaagttag aaggagaagg
     3661 agctacacga agacgtataa tggatatggg gattacaaga ggcgttaata tatttataag
     3721 aaaagtagct cctcttggtg acccaattga ggtaacagtc agagattatg aactatcaat
     3781 tcgtaagtca gatgcagaaa aaattattgt ggaataataa agattaatta gagtagggtt
     3841 aatgtaatta tataatgaga atgattttct atatcatgaa tagtgatgta tttaaataat
     3901 aatggaggga atggaaatgt caataaaaat aggtctcata ggaaatccta actgtggtaa
     3961 gacaacaatg ttcaatgggc ttacaggctc atcacaatat gttggtaact ggccaggagt
     4021 aacagttgaa aaaaaaggtg gaaaattaaa aggaaataaa gatgtggaaa tagtagattt
     4081 accaggtatt tattctttat ctccatatac attagaagaa gtagttacac gtaattttat
     4141 gctagatgat aagccagatg cagtaataaa tattgtagac gcatcaaata tagagagaaa
     4201 tttatatctt acaactcaag ttttagagct aggtatacca accgttatag ctcttaatat
     4261 gatggatatt gtcaataaga atggtgataa gataaatatt aaagaactat ctgaagttat
     4321 aggttgccca gtggtagaag taacagcagt taaaggtcaa ggaattatgg aggctgctga
     4381 aaaggcagtt gaacttgcaa gtagtaacaa taaattaaat ttcaaattac ctttcgttga
     4441 taaaagtaag gaagcaatag aaaaaattga aaaaataata gaagaaaaga ctccatatat
     4501 agatgtagaa acacgctggt tagctataaa gctatttgaa agagatgaaa atgttataca
     4561 aaaacttgat atttctaaag caatattgaa tagtattgaa gaaataacta gaaattgtga
     4621 agatgaattg gatgatgata gtgaaagtat aataacagct aatcgttatg aatttattag
     4681 ttcaataatt tcaaatataa taaagaagaa tagaaaaggt aaggaaactg tatcagataa
     4741 gatagataag attgtaacaa atcgtatact agcattacct atttttgcac ttataatgtg
     4801 gggagtttac tatatagcag taagttcatt gggtactata gcaactgatt ggacaaatga
     4861 tgtactgttt ggagaaatta ttcaaggtaa tgtaagtaat tttctagctt ctttaaatgt
     4921 agcagagtgg ttacaagggc ttgtagttga tggtctaatt ggtggagttg gagcagtatt
     4981 aggatttgta ccacaaatca tgcttttatt cttattatta tcaatattgg aagattgtgg
     5041 gtacatgtcg cgtgtggctt ttataatgga tagaattttc cgtaagtttg gtctttcagg
     5101 aaaatcattt ataccaatgc ttataagttc aggttgtgga gttccaggag ttatgtcaac
     5161 tcgtactata gagaatgata gagatagaaa aatgactatt atgttaacta cattcattcc
     5221 atgtggagct aagattccaa taatagcatt atttgcaggg gcattatttg gaggtgcttc
     5281 atgggtagct ccatcaatgt atttcttagg tattgcaatg ataatcattt gtggaattat
     5341 attaaagaaa actagtttat ttgcaggaga accatctcca tttgttatgg aattacctca
     5401 atatcatatt ccaagtgcaa aaggtgtttt aatacatatg tgggatagag gaaaagcatt
     5461 tataatcaaa gcaggaacaa ttatatttgt tgcttgtgga gtaatctggt tcttacaatc
     5521 atttaattgg tcgttacaaa tggtagatgc tggagatagt atattagcta gcttaggaaa
     5581 tataattgca ccaatatttg ctcctcttgg atttggaaat tggcaatcat cagtagcaac
     5641 agttacaggt ctagtagcta aggaaaatgt agttggaaca tttggagttt tatttggtat
     5701 atcagatgca acagagcaag acccaacact tcttgcttca gttgcaagta tgtttacagt
     5761 agctagtgcg tttgcattta tggcatttaa tatgctttgt gcaccttgtt ttgcagctat
     5821 tggagccata aaaagagaaa tgggttcatg gaaatggaca tggattacat taggattcca
     5881 gactttaaca gcatatataa ttgctcttct tatcaatcaa gttggaagtt tagttttagg
     5941 tactggagga agtattgctg gggcaataat atctatagtt atagcagtag cagtagtctt
     6001 tgtagtttta acttattcaa ataaaaatat gaaaaaagaa aaaatgggta aattaagtta
     6061 tatgaaaaat taattcatat aaataaggag tgatgaatat ggctacattt attatagcag
     6121 ctattgtagt agtattaatg gcactagctg taatgcatat ggttaaaaat tcaaaaaaga
     6181 atggtagcag ttgtggatgt ggttgttctg gatgctcgag tagtaagagt tgtcatagcg
     6241 ctaagagata aataggataa gagcctgata aaaagtttaa attgctttat tatcaggttc
     6301 ttatttttta tataaataac aaatttcata tttgaagtaa gttagactat gttgtaattt
     6361 tattgtataa ttatagacaa atatatgatt aggagggatt attgctaaat taagcaatat
     6421 gagtgaatat tatgtttaaa tttgatgtta caaaggagct tatgtgtata gcaaggggaa
     6481 gtggtaaatt ttttgcaaaa aaaggtgcta tggtagcatt taaaggaaat tttaattttg
     6541 aaaaattgct gctaggtcca agtaatggtg gtggcttggg aagagcatta ttaggtcatg
     6601 ttcagaggtc attgacagga gaacaaatgc caataatggt ggttgaaggt gaaggtgaaa
     6661 tttacctagc tcaaaatgct taccatgtag atgtaatatc aattgaccca ggtgattcca
     6721 tttatgttga gagtgaaaac ttattggcat tttctgaaca attaaattac agtgtaaaat
     6781 tgattggttc aggggtaata tctcaaaaag gtctttttac tacacattta gtaaataaaa
     6841 caggacaaaa tcaagatgtt gctataataa cagatggtaa tcctctaatt cttgaaggac
     6901 cttgttgtgt tgacccagat gcattagtag cttggactgg aagagaacca tatcctaaag
     6961 tatcaaaact atcatggaag acttttatcg gtcagacttc aggagaatct tatcatatgg
     7021 aatttgttga acctggacaa atagtcatta tacaaccatc tgaaagattg tctggcttaa
     7081 aaacagattc tacaccttct gatagaatga gttcaggtgg attaagactt ggcatagatt
     7141 aaatcttatg atatagcttg attattattt aagatattaa aaaataaaaa gggtagcagg
     7201 gatagatatg ttaaaaggag ttttatatgt ttgagaaaat tcataaaagg tttaaaggat
     7261 attatatttt tttaataatt ttaattttat ggaagatagt atgtgttttg ggtatatgga
     7321 gtagctatat tctgcctcct cctgaaatag tcttagatac ttttataaaa atgatatatg
     7381 acggttctat tttcttaaat gtttatgcca gtgtaagaag gatattaata ggttttttaa
     7441 taagttcatt gatggctata ccacttggag ttgtatttgg agtaaataaa aaaatatatg
     7501 aatatttcaa gccactattt gaatttatgc gaagtacacc tccactagct ttggtgccaa
     7561 tgcttatttt atggtttggt ataggtgagg aatctaaaat tataattatt attttggcat
     7621 cattttttcc aatattttta aatacattga aaggcataaa aaattgtgac gataaattaa
     7681 tagaagtagg taaatctttt aatttgtcta gaaagagaat tttttataaa ataataattc
     7741 ctaattctat gctggatatt gttgttggac ttagtttggg gcttgggtat agttttaggg
     7801 ctataattgg agcagagctt attgcagctt cttcaggact aggctatttg atttctgatg
     7861 gaaaagatat gtctagaact gatgttgtct tggtaggaat ttttgttatt ggatttttag
     7921 gtatactttc agattattta ttttcaaaga taataacaaa atttagcaaa gggaagcagg
     7981 ttgatttaaa tggttagaag tggatttttg ctagagaata tatataagaa atatttagtt
     8041 gataataaag agcatttagt tcttgataat atatctttaa atatatctag tgaagagata
     8101 actgttatat taggcgaaag tggatgtggg aaaacaacgc tacttagaat tttagcaggg
     8161 cttgaaaatg ctactaatgg aaacgtatat ttttttaata atgataaaaa atgtactcca
     8221 aaagtaggaa tggtatttca agaaagtaga cttatgccat ggctgaatgt aagtgaaaat
     8281 atacttttac atactgaaaa agataataga aataaagttg atttagacaa gtatttaaaa
     8341 atgatgaagc ttgaaaagtt taaaaactca tatccaaacg aattatcagg aggaatggct
     8401 cacagagtaa gtatagcaag agcgctttcc ttcaatcccg atattttgct gatggacgaa
     8461 ccatttgcag cactagatta cttcacaaga agaaaaatgc aaaaagaagt tgtaaatatc
     8521 cataaaaata ctaaaaaagg tgtagtattt gtaactcaca atattgagga agctatggaa
     8581 atagcaaaga aaataattgt ttttagtaaa aataaaagga ttaaacagtt tagtgtcgaa
     8641 gatgaatata atagagattt aactaaaaac tattatataa acttgaaaaa agaaatatta
     8701 agggaattgg gggagtttta attgattaaa agaaacaaaa agattttagc agtaatgaca
     8761 tctttaataa tggtgttggt tggaactgta ggatgttcaa caacaaatga taagaaagat
     8821 gaacctgcaa aaaaagaggc tagtttacct aaggaaataa atttaactta tgtaaaatca
     8881 ccacttaatg ttcctagtat attagaaaaa aaggatgatt tatttggaaa ggaatttaaa
     8941 aaggacaata taaaagtcaa ctttcatgaa ttgacaacag gaccagagca aactcaagca
     9001 ttagcagcag gtgagctaga ctttttacat gcattaggag gaacgtctgc aataatagct
     9061 gcatcaaatg gtgttggtct taaaattacg aatatttata gtcgttcacc aaaaggatat
     9121 atgcttataa ctaataatga aaatattaaa tctataaaag acttaaaagg taaaaaaatt
     9181 gtaggtccaa aagggactat acttcatcaa ctgttgattg ctgcacttgc taaagaaggc
     9241 tatactatag atgatgttga atatgtaaat atggatattc ctagtgctgc ttctgcatta
     9301 gaaagtaaaa gtgctgatgt agcaatgctt gcaggtccag tggcattgaa aacaataaat
     9361 tcaggtgcca aaatgattgt aaatggagaa ggattagtaa gtggaataat agttactgct
     9421 gtaagtgatg attttgctga aaaatatcct gaattagtaa aaagatttat gaaggttcat
     9481 gaagaaactt taaaatatat gaatgaaaac aaagatgaag tgatggatgt tgtatctaaa
     9541 gaagttggtt tatctttaga tgaaacaaaa gaaatgtatt cttggtatga ctttagttct
     9601 aaaataacgg acaaggatat aaaagaatta gaagatacac aggaattttt gatgtctaat
     9661 ggaatgcaac aaaagaaaat aaatattaaa gatatgttgt acaatcaaaa ttagaatact
     9721 ttaaaacatt aaagtagaaa atacaccctt attatggaag ctgtatagta aaagagggtg
     9781 tatttttatt attatacaaa aacacaatta aaaacaactt actattttat ctacacttat
     9841 gtagtctagt aattttctca ttttcttacg gtgaagtaaa aacttcgaat atgtgtcctc
     9901 ggattgtttt gtgtcaccat aaactttcca atatttaatc atagaaactt cataattact
     9961 atatcccata aatccaatca catctttaag aggatatcct aaaaaaatac ctatttcatc
    10021 aggaaaaaca tcactcttaa gtttatctac taaatgctct aaataagcat taacattcgt
    10081 attttgtttg tatcctaaaa attttaaaaa gtttactgtt tttttacatt ttaatttttc
    10141 tgataatgct tttttattta caaataagat ttttaatcct tttttttctt tatcaataac
    10201 tataaaatct attttcttac atttatatag atatttataa atatcattaa ttttttcttc
    10261 tctatttaca tcagtaaacg aaatatttat tatttcagca ggtttggagc ctaaaaagac
    10321 tggacctaaa acttctaaaa gccactttat atatgatgat tcaattttat ttttacaaca
    10381 aaattgtgaa ttacaacaag acataaaaga taacctcctt caaatgaaaa ttaatatcaa
    10441 gtaattag
//
LOCUS       DAEPXF010000140        10437 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain HB27
            SAMN13376389-rid12479423.denovo.147, whole genome shotgun sequence.
ACCESSION   DAEPXF010000140 DAEPXF010000000
VERSION     DAEPXF010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13376389
            Sequence Read Archive: SRR10518432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10437)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10437)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001069624.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001069624.1
            Genome Coverage       :: 315x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/15/2021 01:54:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,165
            CDSs (total)                      :: 4,076
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 89
            rRNAs                             :: 10, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 10 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 19 of 69
            Pseudo Genes (incomplete)         :: 44 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 10 of 69
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10437
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13376389-rid12479423.denovo.147"
                     /strain="HB27"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="China: Hebei"
                     /collection_date="2017"
     gene            complement(3..701)
                     /gene="yunB"
                     /locus_tag="KRL67_002898"
     CDS             complement(3..701)
                     /gene="yunB"
                     /locus_tag="KRL67_002898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation protein YunB"
                     /protein_id="HBH1523764.1"
                     /translation="MNRIVDRKTKNEFRKIIAIFLVILFLSVFIGSFIYIDKTLRPTI
                     TVLAETKALELANRSINKAVAEMVEGKINYEDLMDIQLDNNGKITMIQANTIMMNEIA
                     SAIALEIQDELKKDKTASSYIPIGTALGSPILAKYGPKLEVSIEPIGTVSVNFKTEFE
                     SSGINQTRHRIYLEAQTQVKVVIPLITSTKQIKAQIPICETIIVGDVPESYVNIPEKN
                     LGNVLPNTGKNTNK"
     gene            915..3608
                     /locus_tag="KRL67_002899"
     CDS             915..3608
                     /locus_tag="KRL67_002899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HBH1523765.1"
                     /translation="MNNDNDKNGNKIRRKKVSSSGNTNKPINRTSANKTRSTKNKKKS
                     KKSDKFKKLRVFGIVFLVLLVVGTAGTAGLVFASLRDVTPVTEAVLDKQTNQTTTIKY
                     ANGKTLSTAPSVNKKTPVPLDKISPYLQHAVIAIEDERFYEHKGVDIKGLFRSVLKTL
                     TGTKQGGSTIPMQVSKMLLTTEQQTIPRKIKDIYYAHEMSKTVSKDKILETYLNNFFV
                     GRGLAGAEAGARGYFDKSAADLTLAESALLAGSTKNPSRFSAYKTSKLEGNETKEDLE
                     NKLLFFVNTTDDDLDDPTQVDFDMIEKIKSWELISNDTYRQLKAGTLVVRKAVSNPEA
                     KKRQEIVLKKMLELKYIKQSEYDEAIKAKIEIKLPQSSDKVSSSVEDLIESEVINALM
                     EQGHTNDEAQNLFYNGGLIVNTTIDPKMQDALEEEFDRNSNFPGHMVGPDGVSQPQAA
                     MVILDYKNGEIRALAGGRNISGRKTLNRATNPHQPGSSIKPLAIYTPAIDTLKITQST
                     ALSDSRGGYKFEENNKWNPRTTTAGHGSMSLRKALAKSSNTIAVKTAEMLGDSYDECV
                     DIMMDYLKNFGITTLKNNHSGSSEASDRKFPSLTLGGMANGITPLQMAAAYGTLANQG
                     VYVEPSIFTTITTFDGQLLVKNTPEEHKVVDPEVAYVVTDMLESVITEGTGGVATLPK
                     GMPVAGKTGTTNSAYDAWFVGYTPYYVGATYIGDDAGRKDDSGNTIKRREVPHGSTST
                     AKLWEKIMEKIHANLTVTEFEVPKNVYFTKINLEDGGKQSSGSKAAFIEGTAPTKVSS
                     QPSSEDTKKPDNNQPEENNNNNNNTGDNGGGSTPPDNGGNNGGGSTTPPDNGGNNGGG
                     SATPPDNGGNNGGGSTTPPDNGGNNGGGSTTPPDGGGTPAT"
     gene            3908..4306
                     /locus_tag="KRL67_002900"
     CDS             3908..4306
                     /locus_tag="KRL67_002900"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HBH1523766.1"
                     /translation="MKWKELYNKARGKLNPRTISPFIDAGGVAAAILTDSGNVYTGVC
                     IDTACTLGMCAERNAIANMITNGESKITKLVCVMSNGSVGSPCGACREYLMQLDKDSA
                     CIEILVNVETEKTVKLSELLPDWWGSDRFI"
     gene            5217..5783
                     /locus_tag="KRL67_002901"
     CDS             5217..5783
                     /locus_tag="KRL67_002901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBH1523767.1"
                     /translation="MPPKPIITKQDIINAAIQLVRENGISSINARNLAKTLNCSTKPL
                     FRIYTNMDEIKKDIKIELDNYYNSFMESKINDENRLLSQGLAYIEFAQNEKMIFNTLF
                     MNMTMKGSSLQDIIHAEWNRITIENAKKVTGLSIEKSEILFINFWLYSHGVATQIISN
                     GIDIPLDMVQQLLENAFERFSLDITKTE"
     gene            5801..6526
                     /locus_tag="KRL67_002902"
     CDS             5801..6526
                     /locus_tag="KRL67_002902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HBH1523768.1"
                     /translation="MLESKIRTPEEIKRYMLDLKEWLAQERSKPVEEMSDFFSKRIDI
                     YDNIHLGNWAEEYAHIADYFDTPLSSLLDIGCGTGLELASIYRKFPNVQITGIDLSKE
                     MMSKLKEKYSDKNIDLILADYFEYPFEQEKYDAALSFETLHHFKYQKKQKIYKKLFQT
                     IKPGGYYVECDYIACCLEEETICLEQYEFKRKENHVPDDVFVHIDIPLTIEHQIHLMK
                     NAGFRAVYVLHEKNGTMIIKAEK"
     gene            complement(6899..7834)
                     /locus_tag="KRL67_002903"
     CDS             complement(6899..7834)
                     /locus_tag="KRL67_002903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1177 domain-containing protein"
                     /protein_id="HBH1523769.1"
                     /translation="MILKQVIEVYDILDKANANGEEVKTYLQGYGEVDVTVKELSSSK
                     GSTDLVKLTIPGKNGKLKGGDAPTLGILGRLGGIGARPEVIGFVSDGDGALVAIAVAA
                     KLLDMQRKGDILDGDVVISTHICPDAPTKEHYPTPFMDSPIDMMTMNENEVDSSCDAI
                     LSIDTTKGNRVINTRGFAISPTVKEGYILKTSDNLLDIMQTVTGKSPFVFPLSIQDIT
                     PYGNHLYHLNSILQPAVATDAPIVGVAITTEMPVAGCATGATNFSDLDSAGRFAIEVA
                     KLFGRNKCDFYDKEEWEMLIKRYGKLNRFQTFGIQ"
     gene            complement(7878..9050)
                     /locus_tag="KRL67_002904"
     CDS             complement(7878..9050)
                     /locus_tag="KRL67_002904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HBH1523770.1"
                     /translation="MECFKEVVNMNELKAKIKEEIKLLSEEKKTSFEKVSDYLFSNPE
                     LAFEEYKSQKALCDLLEENEFNVTKGVGGLETSFEAVYSNGTNGKTVAFLAEYDALPG
                     MGHACGHNIIGTSSVGAGIVLKEIMKKHNIEGTVKVFGTPAEERVGGKITMIKEGVFN
                     NVDAALILHPSDASMPDDISFAQVNLKFDFTGKASHAAAFPWEGKSALSGVIALFNSV
                     NSMRLHLKDYARVHGIITDGGSIHNIIPEKSTAIFNVRALSIEYLNEICEMLKNCAKG
                     AAISTGTNVEIVQLDEIYKEIKNDSELVNIVRQNFEVLGEDYVERDLSQGIGSTDTGN
                     LTHEIPAIQAYIKLKENTATHTDEFAVAAGGEEGRVALIKAIKVLAMCGVDILYSK"
     gene            complement(9037..9501)
                     /locus_tag="KRL67_002905"
     CDS             complement(9037..9501)
                     /locus_tag="KRL67_002905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH1523771.1"
                     /translation="MEKTFKTIMEQIKIVLFIYIIICFGQMIALKTQAQDLLIGSVSG
                     FLLVIIAIIIKNFVKKPNLPGFAWATLVAFVLSLPISPVGDIIATNVGKINFMSTVTP
                     LLAFAGISVGNQLDILKTLSWKLVVISFIVMTSTYFGSAFISQTVLRLNGML"
     gene            complement(9516..10358)
                     /locus_tag="KRL67_002906"
     CDS             complement(9516..10358)
                     /locus_tag="KRL67_002906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3100 domain-containing protein"
                     /protein_id="HBH1523772.1"
                     /translation="MKEKAGIRNAIIAAFIITILAELIGPISFKVMGINVTLLSILWA
                     IFIGMAVSPHLLGRVIPALKKFIGDNEINVSPYLLSLTLYPLGIMFGINAGPKVGILL
                     QAGPALLLQEFGHMGTMLIALPVGVALGLGRSALGGTFSLCRDTALGIIGDKYGLNSP
                     EGIGTLGTYISGSIFGTLLFSFLAPVGVLLGFHPYALAMASGMGSGSMMGAATASLMN
                     TVPQMSEQILAYSATSGLITAVTGIYIELFLSLPVANYYYSKVAPVIERFRGRKKRRT
                     SNKL"
BASE COUNT         3535 a         1782 c         1627 g         3493 t
ORIGIN      
        1 tattacttgt tggtattctt tccagtgtta ggtaaaacat ttcctagatt tttttctgga
       61 atatttacat aactttctgg aacatcacct actattatag tttcacaaat aggtatttga
      121 gctttaattt gttttgttga agttataagt ggtattacta ctttaacttg tgtttgagct
      181 tctaaatata ttctatgtct agtttgattt atacctgaag actcaaattc cgttttaaag
      241 tttactgata ctgttcctat aggttcaata gacacttcaa gtttaggacc gtatttagct
      301 aatataggac ttcctaatgc tgttcctata ggtatgtaag aacttgctgt cttatccttt
      361 tttaattcat cttgtatttc taaagctatt gcagatgcaa tttcattcat cattatagtg
      421 tttgcttgta tcatagttat tttgccatta ttatcaagct gtatatccat taaatcttca
      481 taatttattt ttccttctac catctcagca acagctttat ttatcgacct atttgctagt
      541 tcaagagctt tagtctctgc taaaactgtt attgtcggtc ttaaggtttt atctatatat
      601 ataaaactgc ctataaatac agacaagaaa agaattacta aaaaaatagc tatgattttt
      661 ctaaattcat tttttgtttt tctatctact attctattca aaattatccc ctcctattta
      721 tttatatgta gccttggcat aatttttata atatttgtca aaaataaata aaataaaaat
      781 atagactttt taatatatta tgtttataca tatacaatgt acagaatatt atagcaaatt
      841 cattttttct attaattaca aaactactcc tatttgtgtt ataatattgc attaagtaag
      901 gaggttaaaa tcttatgaat aacgataatg ataaaaatgg aaataaaatc aggagaaaaa
      961 aagttagctc atctggtaat acaaataaac caattaatag aacttcagct aacaagactc
     1021 gttcaacaaa aaacaagaaa aaatctaaaa aaagcgataa atttaaaaaa ctaagagtat
     1081 ttgggatagt atttttagtt ttactagttg ttggtacagc aggtactgct ggtctagtat
     1141 ttgcgtcact aagagatgta actccagtta cagaagctgt tttagataaa cagacgaacc
     1201 aaacaactac tattaaatac gctaatggaa aaacattatc tacagctcca agtgtaaata
     1261 aaaagacacc cgttccatta gataagattt ccccttatct tcaacatgct gtaattgcaa
     1321 tagaagatga gcgtttttat gagcataagg gtgtcgatat taaaggttta tttaggtcag
     1381 tattaaagac ccttacaggt actaagcaag gtggtagtac tattcctatg caggtatcca
     1441 aaatgctgtt aacaactgaa cagcaaacta tacctcgtaa aataaaggat atttactatg
     1501 cacatgagat gagtaaaaca gttagtaaag ataaaatact agaaacttat ttaaataact
     1561 tcttcgttgg tagaggtcta gcaggtgctg aggctggtgc acgtggttat tttgataagt
     1621 ctgcagctga cctcactcta gcagagtcag cacttcttgc tggttcaact aaaaatccat
     1681 caagattttc agcctataaa acttctaagt tagagggaaa tgaaactaag gaggatttag
     1741 aaaataaatt attatttttt gttaacacaa ctgatgatga tttagatgac cctactcaag
     1801 tggactttga tatgattgaa aaaataaaat cttgggagct tatatctaat gatacttata
     1861 gacaattaaa agcaggtaca ttagttgtta gaaaggctgt aagtaaccct gaagctaaaa
     1921 aaagacaaga aattgtttta aagaaaatgt tagaattgaa atatataaaa caaagtgaat
     1981 atgatgaagc aatcaaagct aaaatagaaa taaaattgcc tcaatcatca gataaagttt
     2041 cttcatctgt agaagacctt atcgaaagtg aagttataaa tgctttaatg gaacaaggac
     2101 atacaaatga tgaagctcaa aacttattct acaatggtgg tctaattgta aacacaacta
     2161 tagaccctaa aatgcaagat gctttagaag aagaatttga tagaaatagc aatttcccag
     2221 gacatatggt cggccctgat ggagttagcc aaccacaagc tgcaatggta atcttagatt
     2281 ataaaaatgg tgaaataaga gcactagctg gaggaagaaa tataagtggt agaaaaactt
     2341 tgaatcgtgc tactaaccct catcagccag gttcttctat aaaaccttta gcaatatata
     2401 cacctgctat tgatacccta aaaataacac aatcaactgc tctaagtgat tctcgaggag
     2461 gatataagtt tgaagaaaat aataaatgga atccaagaac tactactgct ggtcatggtt
     2521 ctatgagttt acgtaaagca cttgctaaat cttcaaacac gatagctgtc aaaactgctg
     2581 aaatgcttgg agactcttat gatgaatgtg tagatataat gatggattac ttaaagaact
     2641 ttggtataac aactttaaaa aataatcact caggttcatc tgaagcaagt gatagaaaat
     2701 tcccatcatt gacactaggt ggtatggcga atggtattac tccacttcaa atggctgctg
     2761 cttatggtac attggcaaat caaggtgttt atgtagaacc ttctattttt actactataa
     2821 ctacctttga tggtcaatta ttagttaaaa acactcctga agaacataaa gttgtagacc
     2881 ctgaagtagc ttatgttgtt acagatatgc tagaaagcgt aattactgaa ggtactggtg
     2941 gtgttgcaac acttccaaag ggaatgcctg tagcaggtaa aacaggtact acaaatagtg
     3001 catatgatgc ttggtttgtc ggttacactc cttactatgt tggtgcaact tatattggag
     3061 atgatgctgg tagaaaagat gattctggta acactataaa acgtagagaa gtaccacatg
     3121 gaagtactag tactgcaaaa ctatgggaaa aaattatgga aaaaatacat gctaatttaa
     3181 cggttactga atttgaagtt cctaaaaatg tatattttac aaaaattaat ttagaggatg
     3241 gaggaaagca atcttctggc tctaaagcag catttattga aggtactgct cctactaaag
     3301 taagttctca accatcatct gaagatacaa aaaaaccaga caataaccaa ccagaagaaa
     3361 ataataataa caataataac acaggtgata atggtggcgg ttctacacct cctgataatg
     3421 gcggaaacaa tggtggtggc tctactactc ctcctgataa tggcggaaat aatggtggtg
     3481 gctctgctac tcctcctgat aatggcggaa acaatggtgg tggctctact actcctcctg
     3541 ataatggtgg aaataatggt ggtggctcta ctactcctcc tgatggtggt ggaactccag
     3601 caacttaata aaaaatagta aatatctggc agcgataaaa tttttttttc tagccagaaa
     3661 agcccatgaa atgagcattt ctgctgtgtt cgctacaacc ctgaaacggg taaattggtg
     3721 ctctaatggt gcccaagtga acaaacaggg ttatgaacag cagtatgaag taatgaaacg
     3781 aagtaaaccg tttgacattt tcacgcacga atattagaat gaaaaaagcg agtaatgtgt
     3841 tgtgtgcctt aggcggaaaa ggcgtataaa agattattta tatggataca acaggaggat
     3901 caacctgatg aaatggaagg aactttataa taaggctagg ggaaaattaa atccacgcac
     3961 aatttcacct tttatcgatg ctggtggggt tgccgcagca atccttaccg acagcggaaa
     4021 tgtttatact ggtgtgtgta ttgatacagc ctgtacattg ggaatgtgtg cagaaagaaa
     4081 tgctatcgcc aatatgataa caaatggcga aagtaaaatt actaaattag tttgtgtgat
     4141 gagcaatggg agtgttggct caccatgtgg tgcttgtcgt gaatatttaa tgcaacttga
     4201 taaggatagt gcttgtattg agattttagt taatgtcgaa acagaaaaaa ctgttaaatt
     4261 gagtgagctt ttgccggatt ggtggggttc cgacaggttt atttaacttt atcaaatgag
     4321 tattttgttt catgcacctt attggtataa tacgtacttc ccttttatcc gttctcaata
     4381 gtggcaagca gtacttcatg gtacacacaa aaatatttta caggagtgta tataatcttg
     4441 ccatgattgt tatgattgtt ttcggagatt ccagtttgtc aggagggtag gacctatctg
     4501 tccgttgtac gccctgcttc gtgggcatgg ttcccacggg gcataataaa gagcgcccca
     4561 gatggtagtt gatacctccg gggcgctctt tattatgtcc cacatgggaa ccctatcccg
     4621 gtttcgaggg gatcgcccct gaatggccgc cgccttatag cctcccatag tgcttgcggt
     4681 tttggataaa gtatagtata gtaaacacgt cagcagacca cacaagggct tgttgatttt
     4741 ttgttttgtg gtgcccaact ttacaaaacc acgctgttca aggtcatgcc accttatgat
     4801 ggagaaaata gctatacagg caataaaaaa tagaatttaa ataaaaaata taaggagtta
     4861 tctcaagatg attaaaaaaa tcactttgaa acgctccttt ttttataatt acatcgtatc
     4921 atgtgagatt tcatcctcaa tcaacgcagt tctttctctg ttacccatac aatgatttgt
     4981 acgttgtctt atcgtacaaa gaagctttga cgaagtttca aatttaacta aactacattt
     5041 tactgtttat ctccttttta agatataagt acacatatta tggtatttat tgaaataaat
     5101 catattacac tctatgtctg tagcttttct ttgaagtcta ttgacacagt tatatgcctt
     5161 tgttataata acaacaccag ttgcgcaact taagttaccg aaaggaggat gaaaagatgc
     5221 caccaaaacc gattattaca aaacaagata ttattaacgc tgcaattcag ttggtaagag
     5281 aaaatggaat aagtagcata aatgcccgta acttagcaaa aacccttaat tgctctacaa
     5341 aacccctttt tcgtatttac acaaatatgg atgagataaa aaaagatatt aaaatagaac
     5401 tagacaatta ctataattct tttatggaaa gtaaaataaa tgatgaaaat cgtctgttga
     5461 gtcaaggact tgcatacatt gaatttgctc aaaatgaaaa aatgattttc aataccttat
     5521 ttatgaatat gacaatgaag ggttcatcct tacaagatat tattcatgca gaatggaaca
     5581 gaattactat agaaaatgct aaaaaagtta caggcttatc tatagaaaaa tcagaaatac
     5641 tatttattaa cttttggctt tattctcatg gagtggctac acaaattatt tccaatggta
     5701 tagacattcc attagatatg gtacaacagt tacttgaaaa tgcttttgag cgattttcat
     5761 tggatataac aaagaccgaa taaagaaagg actgttttat atgttggaaa gtaaaataag
     5821 aacacctgaa gaaatcaaaa ggtatatgct tgacctcaaa gaatggttag cgcaagaaag
     5881 aagtaagcca gttgaagaaa tgtcggattt cttttcaaag cgaattgata tttatgataa
     5941 tattcatctt ggtaattggg cagaagaata tgcacatata gcagattatt ttgatactcc
     6001 actatcttct ttacttgata ttggttgtgg tacgggtcta gaacttgcat cgatttatcg
     6061 aaaatttcct aatgtacaaa ttacaggcat tgatttatca aaagaaatga tgagtaaact
     6121 taaagaaaaa tattcagata aaaatattga tttaatcttg gcagattatt ttgaatatcc
     6181 ttttgagcaa gagaaatatg atgctgccct ctcatttgaa acgcttcatc atttcaaata
     6241 tcagaaaaag cagaagatat ataaaaagct ttttcaaaca attaaacctg gaggatatta
     6301 tgttgagtgc gattatattg cctgttgttt agaggaagaa acaatttgtc ttgaacaata
     6361 tgaatttaaa cgtaaagaaa atcatgtgcc tgatgatgtt tttgtccata ttgatattcc
     6421 tttgactatt gaacatcaaa tacacttaat gaagaatgca ggctttagag ctgtgtatgt
     6481 acttcatgaa aagaacggca caatgattat taaagctgaa aaataaactc aaaatatttt
     6541 cctaattgta gcatatcgct tatggacaat ctacaaaatt catagatatg aaatttaaaa
     6601 gagttttata ttcatcccct taaaaactag tgttctacta catagcagga cgaaaaaaaa
     6661 acagtgcgta acattttctt ataatacgca ctgttttcat ttcaatcccg tatatcaaca
     6721 attaggttag ctttaatgtt acactactta ttggcgctta cctaaatata actctcctta
     6781 tttatgcaat caaataaata ttttattttt taagtcttaa actatatctt ttgtttctag
     6841 ttgtttacat agtatagata tttatatcat aaattatata aacttaatat ttttctaatt
     6901 actgtatacc aaatgtttga aatctattta atttgccata tctcttaata agcatttccc
     6961 actcttcttt atcatagaaa tcacacttat ttctaccaaa tagcttggca acttcaattg
     7021 caaatcttcc agctgaatct aaatcactaa aattagttgc acctgtagca catccagcaa
     7081 caggcatctc tgttgtaatt gctactccta ctattggtgc atctgtagct actgctggtt
     7141 gtaaaatact atttaagtga tacagatggt ttccatatgg tgttatatct tgaatagaca
     7201 aagggaatac aaatggagat ttccctgtta cagtctgcat aatatctaaa aggttatcac
     7261 tagtttttaa tatgtagcct tctttaacag ttggtgatat tgcaaatcct ctagtattga
     7321 ttactctatt tcctttagta gtatctatag aaagtattgc atcacaacta ctatcaactt
     7381 cattttcatt catagtcatc atatcaattg gagaatccat aaaaggagta ggatagtgtt
     7441 ctttagttgg agcatctggg caaatatgag ttgaaattac aacatcacca tctaatatat
     7501 ccccttttcg ttgcatatcc aaaagtttag cagccactgc aattgctacc aatgcgccat
     7561 caccatctga tacaaaacct ataacttctg gtcttgctcc aatccctcct agtctaccta
     7621 aaatgccaag agtaggagca tctccacctt ttaattttcc atttttacca ggaattgtaa
     7681 gctttactaa atctgttgaa cctttacttg aagacaattc ttttacagtt acatctactt
     7741 caccataacc ttgtaaataa gtttttactt cttccccatt agcatttgct ttatctaaaa
     7801 tgtcataaac ctctattact tgttttaata tcatgtctaa tccgatttcc tttcttaata
     7861 tattttaaaa ttatatatta tttactatac aaaatatcta ctccacacat tgctagcacc
     7921 ttaattgctt ttataagtgc tactctgcct tcttcaccac cagctgcaac agcaaattca
     7981 tctgtatgag ttgcagtatt ttcttttaat tttatatatg cttgtattgc aggtatctca
     8041 tgagttaaat ttcctgtatc agttgaacct ataccttgac ttaaatctct ttcaacatag
     8101 tcttcaccca aaacttcaaa attttgtcta actatattca ctaattcaga atcattctta
     8161 atctctttgt atatttcatc taattgaact atttctacat tagttccagt agaaattgca
     8221 gctcctttag cacaattttt aagcatttca caaatctcat ttaaatattc tatgcttaag
     8281 gctcttacat taaatatagc tgtagatttt tcaggaatta tattatgtat gctacctcca
     8341 tcagttataa ttccatgcac tcttgcataa tctttaagat gtaatctcat gctatttaca
     8401 ctgttaaata gtgctattac tccacttaag gcacttttcc cttcccaagg aaaagcagca
     8461 gcatgtgatg ccttaccagt aaaatcaaat tttaaattaa cttgagcaaa tgatatatca
     8521 tcaggcattg aagcatcact tggatgtaaa attaatgcag catctacatt attaaataca
     8581 ccctctttaa tcatagttat ttttccacct actctttctt ctgcaggcgt tccaaaaact
     8641 tttacagtcc cttctatatt atgttttttc atgatttctt ttaatacgat acctgcacca
     8701 acagaacttg tacctatgat attgtgacca catgcatgtc ccattcctgg aagtgcatca
     8761 tattcagcta aaaaagcaac tgttttccca ttagttccat ttgaatacac agcttcaaaa
     8821 gatgtttcta atcctcctac acctttagtt acattaaatt cattttcttc taacaaatca
     8881 cataatgctt tttgtgactt atactcctca aatgctaatt ctggatttga aaataaatag
     8941 tcagaaactt tttcaaatga tgtctttttc tcttcagata ataactttat ttcctcttta
     9001 atttttgctt ttaattcatt catatttact acctccttaa agcattccat ttaatcttaa
     9061 aactgtttga gatataaaag cagaaccaaa gtaagtagat gtcataacta taaatgatat
     9121 aactactaac ttccaactca aagtctttaa tatatctaat tggttaccta cagagattcc
     9181 tgcaaaagct aataatggtg ttactgtaga cataaaattg attttcccta catttgttgc
     9241 tatgatatcc cccactggag atattggaag cgataaaacg aatgcaacta atgtagccca
     9301 tgcaaaacct ggtaaattag gttttttaac aaaattcttt attataatag caattatcac
     9361 caacaaaaat ccagatacac ttccaatcaa taaatcttga gcctgagtct taagtgctat
     9421 catttgtcca aagcaaataa taatatatat aaacaatact atttttatct gctccataat
     9481 agtcttaaaa gttttttcca tatatatatc ctcctttaca atttattact tgttcttctt
     9541 ttttttcttc ctctaaatct ttcaataact ggagctactt tgctataata gtagttggct
     9601 accggtaaag ataaaaataa ttctatatat attccagtaa cagctgtaat caatccacta
     9661 gtagctgaat atgctaaaat ctgctctgac atttgtggaa ccgtattcat taatgatgct
     9721 gttgcagctc ccatcattga accactaccc attcctgatg ccattgctaa agcatatgga
     9781 tgaaatccta aaagtacacc cactggtgct aaaaatgaaa ataataatgt tccaaatata
     9841 ctaccactta tgtaagttcc tagtgttcct ataccttctg gtgagtttaa tccatactta
     9901 tctccaataa ttcctagtgc tgtgtcacga cacaatgaaa aagtaccacc taatgcactt
     9961 cttccaagac ctaatgctac tccaacaggt aatgctatca acattgtacc catatgacca
    10021 aattcttgta ataaaagtgc tggtcctgct tgaagtaata taccaacttt aggccctgca
    10081 ttaattccaa acattatccc taatggatat agtgtcaaag ataataaata tggagataca
    10141 tttatttcat tatcccctat aaactttttt aaagctggta taactctccc taataaatga
    10201 ggagaaacag ccattcctat gaatatagcc catagtatac taagtaaagt tacattaatt
    10261 cccataactt taaatgaaat aggtccaatt aattctgcca atattgtgat tataaaggct
    10321 gctattatgg catttctaat ccctgctttt tctttcattt ctatacctcc ttgtaatttt
    10381 tataattttc ttataatata attgttagtg attttattat ataacctatc attgata
//
LOCUS       DAEPXF010000141        10269 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain HB27
            SAMN13376389-rid12479423.denovo.148, whole genome shotgun sequence.
ACCESSION   DAEPXF010000141 DAEPXF010000000
VERSION     DAEPXF010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13376389
            Sequence Read Archive: SRR10518432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10269)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10269)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001069624.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001069624.1
            Genome Coverage       :: 315x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/15/2021 01:54:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,165
            CDSs (total)                      :: 4,076
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 89
            rRNAs                             :: 10, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 10 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 19 of 69
            Pseudo Genes (incomplete)         :: 44 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 10 of 69
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10269
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13376389-rid12479423.denovo.148"
                     /strain="HB27"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="China: Hebei"
                     /collection_date="2017"
     gene            225..1649
                     /locus_tag="KRL67_002907"
     CDS             225..1649
                     /locus_tag="KRL67_002907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICB"
                     /protein_id="HBH1523773.1"
                     /translation="MSSSNQSTIKTSIQRVGKFLSGMVMPNIGAFIAWGLITALFIPT
                     GWMPNEKLSTIGDPMIKYLLPLLIAYTGGKAIAGQRGGVIGAAAAMGVIVGADIPMFI
                     GAMIMGPFAGWVIKKFDKFVDGKVPTGFEMLVNNFSIGIIGMLLAILGFYAIGPAIVA
                     GTALIESGVQFIVSKSLLPLVSVFIEPGKVLFLNNAINHGILGPIGIAEAKEAGKSIM
                     FLLESNPGPGLGVLLAYWMFSRGSVKQSAPGAVIIHFFGGIHEIYFPYILMNPVLILA
                     TMAGGAAGILTFSILGAGLVAAPSPGSIFALMALAPKGGLLPVLAGVAVATVVSFLVA
                     APFVKRASANESEEDSTSLEEAKAKMSDMKSASKNSEKNIEKKQLEVNEIKKIVFACD
                     AGMGSSAMGASRFKNRIKNLDLDIEITNSSVDNLPDDTQIVVTHNTLVERVAKNNSSV
                     EIVSINNFLNDPNLDTLFKRLESK"
     gene            1684..3813
                     /locus_tag="KRL67_002908"
     CDS             1684..3813
                     /locus_tag="KRL67_002908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HBH1523774.1"
                     /translation="MKKKKITSRQKKIILMIVENSKKNIPITISEIAGTLELSSRTVL
                     RDMSGIEKWFDENDFNFVKKPGVGLILEENIENQNFIIELLEEEKIEKEYSKEERNLI
                     ILSKLLVSNEPVKSYYFAKILKVSEGVLNNDFALASKWLERFDIELVRKPGLGVYLKG
                     QEKSFREAYVNLIYDSFNEKEILDMVRNISENIQTDKAIEILSENRLLNLMDRCIIRK
                     VEATLTKKLSDLDVNLADSAYIGLVVHISLALQRIKNGENITMDKEFLKELSITEEFK
                     LAKEIVKGMETDFNMDIPVDEVGYITMHIRGAKQRSSSNHKALNLDDIEIMEITNKMI
                     DLAEDEFKISLKNDERLFKDLANHLGPSINRLNMGLEIRNPLLDEIKNKYSYAYDGVE
                     KISRIIKDKLNINSIPESEIGYIAMHFASAIEKNLMMNTNINIVVACPTGIGTSRFLS
                     TKIENKFPNLNILETISAINIDEEYLKEKDVDLIVSTVELNTSLNYICVGPFMSLDDE
                     QIIKEKIKSIAQNKLINLNTKNDTKNKNKVYEQITESMNIGKDILQFLEEIRFEKFES
                     KDLSELIEDSSKIFAKSSEDIMSIKESLKERLKISIPYIEESKILLLHCMSERIDIMK
                     LSIIKLENRIVLDSNEEIDNVVFMLLPKNSPSYQRQIMSEISGSLIDNIIFTNKINKF
                     SIEEMTLEVKDIVFNFYTNRLKTFIDK"
     gene            3843..4265
                     /locus_tag="KRL67_002909"
     CDS             3843..4265
                     /locus_tag="KRL67_002909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBH1523775.1"
                     /translation="MSKVLNDNNIFLGLDSVSKEEAITLAGRKLVENGYVKEEYIPAM
                     LEREKVMTTYMGMGVAIPHGVNEAKKEILSSGIVILQFPNGIDFDGEKAYLLIGIAGV
                     GDEHLEILSNIAVVLDDDLTERLKNSNDKQAFMEAFAN"
     gene            4284..5435
                     /locus_tag="KRL67_002910"
     CDS             4284..5435
                     /locus_tag="KRL67_002910"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HBH1523776.1"
                     /translation="MKKAIQFGAGNIGRGFIGGLLVKSGYHVVFADVNEEILNSINKD
                     KKYTIHIRDVECIDEVIDNISAVSSIKEEIIDEIVQAEIITTAVGPLVLTKIASTIAK
                     GIKTRKEKGLTSNLNIIACENAIYASSSLKEEVLKYLNKEEVEYLEIYVGFPNCSVDR
                     IVPPGKNENPLDVTVENFYEWNVEKQGFKGEIPTIVGMNLADNLMAYIERKLFTLNTG
                     HAITAYIGYLKGYKTIEESIKDKFICDIVKSAMVESGEGLIKKYNFDSEAHYKYIDKI
                     LNRFKNPYLNDDVLRVGREPLRKLSDKDRLIKPLMTAKSYGLSVDNLILGIGAALHYN
                     NSEDTQSVELQELIKSNGVKKAVAKIANISNDEELLNNIEKSYIFMKNL"
     regulatory      5541..5642
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            5825..6397
                     /locus_tag="KRL67_002911"
     CDS             5825..6397
                     /locus_tag="KRL67_002911"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HBH1523777.1"
                     /translation="MKALQEKILREGSVSGNDILKVDSFLNHQIDVAFLNEIGREFKE
                     RFKEEKVDKIFTIEASGIAIASIVSQYFDNAPVVFAKKSESKNLDKDVYETNVYSFTK
                     AREYSVKVSKKYINKGENILIVDDFLANGRAALGLKDLIEQAEANLVGVGIVIEKGFQ
                     AGGALLKANDVRLESLAVVESIDNGTVKFR"
     gene            6461..7111
                     /gene="fsa"
                     /locus_tag="KRL67_002912"
     CDS             6461..7111
                     /gene="fsa"
                     /locus_tag="KRL67_002912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HBH1523778.1"
                     /translation="MRFFIDTANIEEIKEANDLGVICGVTTNPSLIAKEGRDFIEVVK
                     EISEIVDGPISAEVISLEHKGMIEEAEKLSKIHKNIVIKIPMTAEGLKAVKVLSSKGI
                     KTNVTLIFSAGQALLAARAGATYVSPFVGRLDDISQNGLDLIEEIVDIFSVNSIEAQI
                     IVASVRNPIHVLQAARMGADIATVPLKVINQMIKHPLTDKGIDSFMKDWEGASLKL"
     gene            7263..10187
                     /locus_tag="KRL67_002913"
     CDS             7263..10187
                     /locus_tag="KRL67_002913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBH1523779.1"
                     /translation="MDKILREIQREDKKNPYTDQELAEMLNVARSEVISVRKKNNILD
                     SRERRKKILIKDISKIVSENPQMSERKITEILIENGYNISRSAVSKLLKEENLNQSKI
                     GIKDKEPASSGKYVKVSKVVEEDGFEELIGIKGSLKEKVNLAKSAIMYPPNGLHTIIY
                     GETGVGKSELATCMYKYAIKNNIKEENSPFIVFNCADYAENPNLLIAQLFGVVKGAYT
                     GADANREGLVEQANNGILFLDEIHRLPAAGQEILFSLIDRGEFRRLGESSSNRKANVL
                     IISATTENPDSNLLQTFRRRIPMVINLPNMSERPKSERYEIIIKFFEREAKRVNKNFI
                     ITKEVMCALMNYKCTGNIGQLKSDIQVTCARAFSKSIFSSDKVIIDLDSLRDYIKSGY
                     YDCNCKNEKYSEFSVEDIYIDISELVSGKNRILSDNEISEIYNYAEKELKVLESKCFS
                     EEELKNAFIEKLDKKFNAIKNNKNLSDRRSRIDWGGQLKESTLEIMDKVIVFIKEQFK
                     HVNQGLYLALAIHIEHAIGRIKDGKTIINPSLDKIKASMPMEYELSRYIMGIVEDLTQ
                     VTFPEDELGYLAYYINKFCYNEESIKDKVKVVIVTHGKVGIEMSKVVNHILGIECTLG
                     IEIALTDSPSEGIEHVLEELQKIEARKGILVLIDMGSLVILGDEVEKRLGIRCKTVNR
                     VDTLLAMEAGKLATIEGKSLDGIIADLKKNKNYAMINTNKFSYRKNEYGKKNVIITLC
                     LSGVGTALNLKEHIEKQIKEYDTLIEVKPVAFLNNNLEEELNDIEAEYNIIAICGTID
                     IDYKNVPFISYNEVLGKNGINKVLEHLNSKQPALNIENQLNNLIHEDLILYNFEGISK
                     EYIIDTLVSKLEEGGYVDSKYILSVYKREAMGSVVMASKVAVPHGLPENVIKPAIAIA
                     RLNKPIVWDNKFMVDLVVLLALKENNKKEIRSLFSKINDQHTLEVLLNTEDKNEIKEL
                     LS"
BASE COUNT         4218 a         1009 c         1938 g         3104 t
ORIGIN      
        1 ttaaatttaa atattttata aaaatagtat tatttacaaa atcatatact ccaaaatact
       61 acataaataa aattaaacaa aaactaaaat gacaaatgtc attatctaaa gtgacataga
      121 aacggtttct taaagttgtt actataaaat ataaactcta taataaaagt ataacaaaag
      181 ggaatcatat cgatatagaa tataattcaa gaggaggtaa aattatgagt agttctaatc
      241 aatcgacaat aaagacatct atacagcgag tagggaaatt tttaagtggt atggtaatgc
      301 caaatatagg agcttttata gcatggggtc ttataacagc attatttata ccaacaggat
      361 ggatgcctaa tgaaaaattg agtactatag gcgacccaat gataaaatac cttttaccac
      421 tattgatagc ttatactggt ggtaaagcaa tagcaggaca aagaggtggc gtaataggtg
      481 cagcagcagc tatgggagta attgttgggg cagatatacc aatgtttatc ggagcaatga
      541 taatgggacc atttgcagga tgggtaatta agaagtttga taaatttgta gatggaaagg
      601 taccaacagg atttgaaatg ttagtaaata acttttcaat aggtataata ggtatgcttc
      661 ttgcaatact aggattttat gcaattggac cagcaatagt agcagggaca gctcttatag
      721 aatctggagt tcaatttata gttagcaaaa gtttactgcc tttagtttca gtatttatag
      781 aacctggaaa agtattattc ctaaacaatg caataaatca tggtatatta gggcctatag
      841 gtatagctga ggcaaaagaa gcaggtaaat ctataatgtt cttactagaa agtaaccctg
      901 gaccaggttt aggagttcta ttagcatatt ggatgtttag tagaggaagt gtaaaacaat
      961 cagcaccagg agctgtaata atacatttct ttggtggtat ccatgaaatc tacttcccat
     1021 atattttaat gaatccagtt ttaatattgg ctacaatggc aggtggagca gcaggaatat
     1081 taactttttc aatattagga gctgggcttg tagcagcacc atcaccagga agtatatttg
     1141 cacttatggc actagctcca aagggaggat tacttccagt attagcaggg gttgcagtgg
     1201 caacagttgt atcattctta gtggcagcac catttgttaa gagagcttca gcaaatgaaa
     1261 gtgaagaaga cagtacctct ttagaagaag ctaaagcaaa aatgagtgat atgaaatctg
     1321 caagtaagaa ctctgagaaa aatattgaga aaaaacagct tgaagtaaat gagattaaga
     1381 agattgtatt tgcatgtgat gcaggaatgg gttcgagtgc aatgggcgct tctagattta
     1441 aaaatagaat aaaaaactta gatttagata tagaaattac aaattcatct gttgataatc
     1501 ttccagatga tacacaaata gtagtaactc acaatacatt agttgaaaga gttgctaaga
     1561 acaattcaag tgttgagata gtttcaataa acaatttttt aaatgaccca aatttagata
     1621 cattatttaa aagattagaa tctaaataaa aataaaaagt accactaatt aggagtggat
     1681 aatttgaaaa aaaagaagat tacatctagg caaaaaaaga taatactaat gatagtagaa
     1741 aattcaaaga aaaacattcc aataactatt tctgagattg cagggacgtt agagttaagt
     1801 tcaagaactg tacttagaga tatgtctgga attgaaaaat ggtttgatga aaatgatttt
     1861 aattttgtaa aaaaaccagg agtgggattg attttagagg aaaatataga aaatcaaaac
     1921 tttataattg agttgttaga agaagaaaaa atagaaaaag aatacagcaa ggaagaaaga
     1981 aatttaatta tattatcaaa gctactagtt tctaacgagc cagtaaaatc ttattatttt
     2041 gcaaaaatat taaaggtatc tgaaggggta ctgaataatg attttgcatt agcaagtaaa
     2101 tggttggaaa gatttgatat tgagcttgtt agaaaaccag ggcttggtgt ctacctaaaa
     2161 ggtcaagaaa aaagctttag agaggcatat gtaaatctta tatacgattc attcaatgaa
     2221 aaagaaatac tagatatggt cagaaatata agtgaaaata tacaaacaga taaagcaata
     2281 gaaattttaa gtgaaaatag acttttaaat ttaatggata ggtgcataat aagaaaagtt
     2341 gaagctacat taacaaagaa gctttcagat ttagatgtaa atttagctga tagtgcatat
     2401 atagggcttg tagttcatat ttcattagcc ttacagagga taaaaaatgg tgaaaatata
     2461 actatggata aagagtttct aaaagaatta tctataacag aagagtttaa attagctaaa
     2521 gaaatcgtaa aaggtatgga aacagatttt aacatggata taccagtaga tgaggttgga
     2581 tatataacta tgcatataag aggtgcaaag caaaggtcat cttcaaatca taaggcttta
     2641 aacttggatg atatagaaat aatggaaatt acaaataaga tgatagattt ggctgaagat
     2701 gaatttaaaa taagtttaaa aaatgacgag aggttattta aagacctagc aaatcactta
     2761 ggaccttcta taaatagatt gaatatggga ttagaaataa gaaatcctct tttagatgaa
     2821 ataaaaaaca agtattcata tgcctatgat ggagttgaaa agatttctag aattataaaa
     2881 gataaactaa atataaattc aatacctgaa tcagaaatag gatatattgc aatgcacttt
     2941 gcatcagcca tagaaaaaaa tcttatgatg aatactaata taaatattgt tgtggcatgt
     3001 cccactggaa taggtacttc aagattctta tcaactaaga ttgaaaataa atttcctaat
     3061 ttaaatatac ttgaaaccat atcagctata aacatagatg aagagtattt aaaggaaaaa
     3121 gatgttgatt taatagtgtc tacagttgag ttaaatacaa gtttaaatta tatatgtgta
     3181 ggtccattta tgagtttaga tgatgaacag ataatcaagg aaaaaataaa atctatagct
     3241 caaaataaat taattaattt aaatacaaaa aatgatacta aaaataagaa taaagtatat
     3301 gaacagataa cagaaagtat gaatatagga aaagatattc ttcaattttt agaggaaatt
     3361 agatttgaaa aatttgaaag taaagattta agtgagctta ttgaagactc atcaaaaata
     3421 tttgcaaaat ctagtgaaga tataatgtct ataaaagaat cgttaaaaga aagattaaaa
     3481 atctcaattc catatataga agaatcaaaa attctattgt tacattgtat gagtgaaaga
     3541 atagatatta tgaagttatc aataataaaa ctggagaatc gaatcgtgct agattcaaat
     3601 gaagaaatcg acaatgtagt ttttatgtta cttcctaaaa attcaccaag ttatcaaaga
     3661 caaataatga gtgaaataag tggttcatta atagacaata taatttttac taataagata
     3721 aataaattct ctatagaaga gatgacatta gaggtaaaag atatagtttt taatttttat
     3781 acaaacagat taaaaacatt tatagataaa taaaattaat attaaaaagg aaggttgata
     3841 ctatgagtaa agtattgaat gataataata tatttttagg gttagatagt gtttcaaagg
     3901 aagaagctat aactttagca ggaagaaagt tagttgaaaa tggatatgta aaagaagaat
     3961 atataccagc aatgctagaa agagaaaaag taatgactac atatatggga atgggagttg
     4021 ctattccaca tggtgtaaat gaagcaaaaa aagaaatatt atcttctgga atagttatac
     4081 tacaattccc taatggtata gattttgatg gagaaaaagc atacttatta ataggaatag
     4141 ctggtgttgg agatgaacat ttagaaatac tatctaatat agcagtagtt ttagatgatg
     4201 acttaacaga aagactaaaa aattcaaatg acaaacaagc ttttatggaa gcttttgcca
     4261 actaatctta ggagggattt aaaatgaaaa aggcaatcca gtttggagca ggaaatattg
     4321 gaagaggttt tattggaggc ttgttagtta agtcgggtta tcatgtagta tttgctgatg
     4381 taaatgagga gatattaaac tcaataaaca aagataaaaa gtatactata cacataagag
     4441 atgtagaatg tatagatgaa gtaattgata atataagtgc ggtttcatct ataaaagaag
     4501 aaataataga tgagattgta caggctgaaa taataactac agcagttgga cctttagttt
     4561 tgactaaaat agcctctaca attgcaaaag gtataaagac tagaaaagaa aagggattaa
     4621 caagtaatct aaatataata gcctgtgaaa atgcaatata cgcaagttct tctttaaaag
     4681 aagaggtact taagtattta aataaggaag aagtagaata tttagagata tatgttggtt
     4741 ttccaaactg ttcagttgat agaatagttc ctcctggtaa aaatgaaaat cctcttgatg
     4801 taacagttga aaatttttat gagtggaatg ttgaaaaaca aggatttaaa ggggaaatac
     4861 caactatagt tggaatgaat ttagctgata accttatggc ttatatagag cgtaaattgt
     4921 ttacactgaa tacaggccat gctataacag cttatatagg atatttaaaa ggatataaga
     4981 ctatagaaga gagtataaaa gataaattta tctgtgatat agtcaaaagt gcaatggtag
     5041 aaagtggaga aggattgatt aaaaaataca attttgactc agaagcgcac tataaatata
     5101 ttgataaaat tttaaataga tttaaaaatc catatcttaa tgatgatgta ttaagagttg
     5161 gaagggaacc attaagaaaa ctaagtgata aagatagatt gattaaacct ttaatgacag
     5221 caaaatcata tggattatca gttgacaatt tgattcttgg aataggtgca gcgcttcatt
     5281 ataataatag tgaagatact caaagtgtag aattacaaga gttgataaag tctaatgggg
     5341 taaaaaaagc tgttgctaaa atagctaata taagcaatga cgaagaactt ttaaataata
     5401 tcgaaaaaag ttatatattt atgaaaaatt tataaattta cttgaaaaaa agtagttctg
     5461 ataaagtaac tataatatat tattctacac agtttgaatt atttgttgaa taatagagaa
     5521 ttataatata taattaaaag gaataaatta atatatttac ctgtatatgt ttgataatat
     5581 ggttcaaatg tttctactaa gctaccatta atagcttgac tacaggagtg aagaagatat
     5641 agtttatact tattttaaat tatatattaa gttaattact ttgaatttta agctcctgtc
     5701 tatgatatag acaggagctt ttttagtatt ttaatataaa atacttacaa gaatagatag
     5761 caaaattaga acaaatataa tttttttatt aatataactt tagaaagggc taggtggaag
     5821 ttcaatgaag gctttacaag aaaagatatt gagagaaggc agtgtttcag gaaatgatat
     5881 actaaaagtt gatagttttt taaaccatca aattgatgta gcttttttaa atgaaatagg
     5941 aagagaattt aaagagagat ttaaagaaga aaaggtggat aagatattta caatagaggc
     6001 ttcaggaata gctatagcat caattgtttc acagtatttt gataatgcac ctgtagtatt
     6061 tgcaaaaaaa tcagaatcta aaaatttgga taaagatgta tatgagacta atgtatattc
     6121 atttactaag gcaagagaat attcagttaa agtttctaaa aagtacataa acaaaggtga
     6181 aaatatattg atagtggatg attttttggc aaatggaaga gctgctcttg gacttaaaga
     6241 tttaattgag caagcagaag ctaacttagt tggagtagga attgtcattg aaaaaggatt
     6301 tcaagctgga ggagcacttt taaaggctaa tgatgtaaga ttagagtctt tggcggtagt
     6361 tgaatctata gataatggaa ctgtaaaatt taggtaattt atgatattat atacttgtgt
     6421 ttagcacata ttttaactat gtaattatgg aggatttttt atgagatttt ttatagacac
     6481 agccaatatt gaagaaataa aagaagcaaa tgatttaggt gtaatatgtg gtgtaactac
     6541 taatccatct ctaatagcta aagaaggtag agattttata gaggtagtta aagaaataag
     6601 tgaaatagta gatggaccaa taagtgcaga agtaataagt ttagagcata aaggtatgat
     6661 agaggaagca gaaaaattat ctaagataca taaaaatata gttataaaga tacctatgac
     6721 agcagaaggt ctaaaagcgg tgaaagtact atcaagtaaa ggtataaaga ctaatgtaac
     6781 acttatattc tctgctggac aagctctttt agctgctaga gctggagcta catatgtaag
     6841 tccttttgtt ggtaggttgg atgatatatc acaaaatggt cttgatttaa ttgaagaaat
     6901 cgttgatata tttagtgtaa atagtattga ggctcaaata atagttgcta gtgttagaaa
     6961 tccaatacat gttttacaag ctgctagaat gggagcagat atagcaacag ttcctcttaa
     7021 agttataaac caaatgatta agcatccttt gacagacaaa ggaatagata gctttatgaa
     7081 ggattgggaa ggtgcttctt taaaattata attataaact acgctttata agtatattaa
     7141 agaggttatt atggaaattc taagtaataa ggctaggcta ttttgcctag tcttattttg
     7201 gtgtataata tattgaaaca tattattgaa atagattaat tttttaagtg aggaggcgaa
     7261 aaatggataa aatattaaga gagatacaaa gagaagataa aaaaaatcca tatactgacc
     7321 aagaacttgc tgaaatgtta aatgtagcta ggtctgaagt gatatcagtg aggaaaaaaa
     7381 ataatatttt agattcgaga gaaagaagaa aaaaaatatt aataaaagat atatcaaaaa
     7441 ttgtaagtga aaatcctcaa atgtctgaaa gaaaaataac tgagatatta atagagaatg
     7501 gatataatat atcgagaagt gcagtttcaa aattattaaa agaggaaaat ttaaatcagt
     7561 caaagattgg gattaaagat aaagagccag catcatcagg aaagtatgtg aaggtgagta
     7621 aagttgtaga agaagatgga tttgaagaat tgataggtat aaagggaagt ttgaaagaaa
     7681 aggtaaatct agctaagtca gcaataatgt atcctcctaa tggccttcat actataatat
     7741 atggagaaac aggggtaggt aaaagtgaat tagctacatg tatgtataaa tatgctataa
     7801 aaaacaacat caaagaagag aacagcccat ttatagtgtt taactgtgca gactatgcag
     7861 aaaatcctaa tctattgata gctcaactct ttggggtagt aaaaggtgca tatacaggag
     7921 ctgatgcaaa tagagaaggt cttgtagaac aagctaataa tggaatattg tttttagatg
     7981 aaattcatag attgcctgct gctgggcaag aaatcttatt ttcattaata gatagaggag
     8041 aatttagaag gttaggagaa agtagttcta atagaaaggc aaatgttctc ataataagtg
     8101 ctacaacaga aaacccagat tctaatttac ttcaaacatt tagaagacgt ataccaatgg
     8161 taataaattt acctaatatg agtgaaagac caaaatctga aagatatgaa ataataataa
     8221 agttttttga aagagaagca aagcgagtaa acaagaattt tataataact aaagaagtta
     8281 tgtgtgcact tatgaattat aagtgtactg gaaatatagg tcagttaaag agtgatatac
     8341 aagtaacttg tgctagagct tttagcaaat caatattttc tagcgataag gtaattatag
     8401 atttagatag tttaagggat tatataaaaa gtggatatta tgattgtaat tgtaaaaatg
     8461 aaaagtattc tgagttttca gttgaggata tatatataga tatcagtgaa ttggtgagtg
     8521 gaaaaaatag aattttatct gataatgaga taagcgagat atataattat gcagaaaaag
     8581 agcttaaggt tttggaaagt aagtgctttt cagaagaaga gttaaaaaat gcatttatag
     8641 aaaagttaga taagaagttt aatgctatta aaaacaataa aaatttatct gatagaagaa
     8701 gtcgaataga ctggggagga caattaaaag agtctacatt ggagataatg gataaggtaa
     8761 ttgtatttat taaggagcag tttaagcatg taaatcaagg attgtacttg gctctagcaa
     8821 ttcacattga gcatgctata ggtaggataa aagatggaaa aactataata aatccgagtt
     8881 tagataaaat aaaagcatca atgccaatgg aatatgaatt gtcaagatat ataatgggta
     8941 tagtggaaga tttgacacaa gtaacatttc cagaagatga acttggatat ttggcttatt
     9001 atataaataa attttgttat aatgaggaaa gtataaaaga taaagttaaa gtagtaatag
     9061 ttacgcatgg taaagttgga attgaaatgt ctaaagttgt aaatcatatt ttaggtatag
     9121 aatgtacact aggtatagaa atagcactta cagattcacc atcagagggg atagagcatg
     9181 tattagaaga gcttcaaaag atagaagcta gaaaaggtat tctagttcta attgatatgg
     9241 gttctttggt aattcttggt gatgaggttg aaaaaaggct tggaattaga tgtaaaactg
     9301 taaatagagt agatacgtta ttagcaatgg aagcaggaaa acttgcaacc attgaaggaa
     9361 aaagtttaga tggtataata gcagatttaa agaaaaataa gaattatgcc atgataaata
     9421 caaataaatt tagttacaga aaaaacgagt atggaaagaa aaatgtaatc attactttat
     9481 gtttgagtgg tgtaggtaca gctttaaatt taaaagagca tatagaaaaa caaattaagg
     9541 aatatgatac tttaattgag gtaaagccag ttgctttttt aaataataat ttagaagaag
     9601 agttaaatga tattgaagct gaatataata ttattgctat ctgtggaact atcgatatag
     9661 attataaaaa cgtacctttt atttcttata atgaggtact tggtaaaaat ggtattaata
     9721 aagtattaga gcatttaaac agtaaacaac cagctttaaa tattgaaaac cagttgaata
     9781 atttaataca tgaagatttg attttataca attttgaagg aatttcaaag gagtatataa
     9841 ttgatactct tgtttcaaag cttgaagaag gtggatatgt tgactcaaag tatatattaa
     9901 gtgtatataa aagagaagca atgggaagtg ttgtaatggc ctctaaggta gccgtgccac
     9961 atggacttcc agaaaacgtt ataaagcctg caatagcaat cgcaaggttg aataagccta
    10021 tagtgtggga caataaattt atggtggatt tggtagtact actggcactt aaagaaaata
    10081 ataagaaaga aatacgaagc ttattttcaa agattaatga ccagcatact ttagaagttt
    10141 tattaaatac tgaagataaa aatgaaataa aagaactttt aagctaatta ttagtacaga
    10201 gaagaaagac aaaaaatttg ctttagtata aaatttaaaa ctacttaatt gctaagtagt
    10261 taattactc
//
LOCUS       DAEPXF010000142        10033 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain HB27
            SAMN13376389-rid12479423.denovo.149, whole genome shotgun sequence.
ACCESSION   DAEPXF010000142 DAEPXF010000000
VERSION     DAEPXF010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13376389
            Sequence Read Archive: SRR10518432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10033)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10033)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001069624.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001069624.1
            Genome Coverage       :: 315x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/15/2021 01:54:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,165
            CDSs (total)                      :: 4,076
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 89
            rRNAs                             :: 10, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 10 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 19 of 69
            Pseudo Genes (incomplete)         :: 44 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 10 of 69
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10033
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13376389-rid12479423.denovo.149"
                     /strain="HB27"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="China: Hebei"
                     /collection_date="2017"
     gene            104..1534
                     /locus_tag="KRL67_002914"
     CDS             104..1534
                     /locus_tag="KRL67_002914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBH1523780.1"
                     /translation="MSSQESKPKGNLISNFRDDMRNPKKRAIYTYSSSINLAYNFIGY
                     FVSTVTNIFLTDVAKLGVIMAGGMQIIQSFIKIAVAPITGTVFDKQPFKKGKYYPWLR
                     YAPAALALTYGIFFIVPLMKISPALLLPLMTALLLIASCAQQVVFTIIYAIYPVVAKT
                     PRDRVIGSTTTNIFKEVGKFLVGFSYPLLLVLFADVLGGEGMGYLGTYLFFAAGCLLV
                     FWFSSTEIIKSGAEEEVLQKEEKPKIKASEMAKALFTNVPLMMAFCLEFLICIRSIGL
                     GPLAPYYFKYVIEDERGLAVFLSIMPLVSVAFMFFAPVFIKICREQKLASIISFSICA
                     LCHLLVAWTPWGKTTLGVTMLLAIGGGFSNVVSIINLNFFAGSCDYGHWKSGKDLPGL
                     SMSLYPVAIQVGVLLATTIRTVLMNSMGYQADMVVTEAVKSGFINMISYSMAIPLIIA
                     VVIAILYPVSDKKLNQIREELNRRNA"
     gene            1604..2740
                     /locus_tag="KRL67_002915"
     CDS             1604..2740
                     /locus_tag="KRL67_002915"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HBH1523781.1"
                     /translation="MKIHIQRGLLKMIEKLLRNTVKELHQYVPGEPIEKVKEKYGVKE
                     IIKLASNENPLGPSPKAMETMIEMLKQGQLYPEPEANELRRKLAEKLDLKPENFIVAN
                     GADNVITLIGEAFINRGDEVIYCNPTFPSYRTATIKNEGIPVEVPLTEDYKYDLQGIL
                     DRITDKTKLICVCNPNNPTGTIVDDKELEEFLKKVPENIITILDEAYIEFLTVPNYVD
                     GLKYVRENYNVIVTRTFSKIYGLAGLRVGYGIAKDEIIRTLFTVKEPFSANRVAISGA
                     TAALDDEEFIKETYELNRVGMAYFKEEFTKMGFDVVDSQSNFLYVDMKTDIPKLFEDL
                     KKRGFVIRPSATHARVSIGTMEENKKFVAVLKEILKESQVLDNR"
     gene            2748..2984
                     /locus_tag="KRL67_002916"
     CDS             2748..2984
                     /locus_tag="KRL67_002916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HBH1523782.1"
                     /translation="MKRGVDMNLKINAERCKSCEYCVISCKKGALKISSKINKEGYAH
                     VEVDESKCILCGICYQVCPDNVFEIIKEEACQEA"
     gene            3001..4050
                     /gene="vorB"
                     /locus_tag="KRL67_002917"
     CDS             3001..4050
                     /gene="vorB"
                     /locus_tag="KRL67_002917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate dehydrogenase subunit
                     VorB"
                     /protein_id="HBH1523783.1"
                     /translation="MKKMWKGNHAIAEAALRGGCEFYAGYPITPQTEVMEFLSHRMSE
                     LGRTFIQSENEMAAIYMVYGAYASGMRSMTSSSGPGISLKQEGISYLCANHYPCVIVN
                     VQRWGPGLGSLDSAQTDYLRDTRGGGNGDYHLIVYAPNSIQETVDLMYNSFDIAEKYR
                     VPVEILTEAALGQMMEPVEFPEFKKREDDLGWTYDGSNRDHAKVADNQKPTFCMEKRI
                     RITENEQQWEDYQVEDAEYVFVAFGIPSRTTMNAVRRLREQGEKVGIIRPITVWPFPY
                     KAFEKVNKDVKGFISVESTDTGQLVEDVALASKKVCKENVPVYGLFSGNHIPKTLQVM
                     DTYSKIKSGEIKEVF"
     gene            4052..4792
                     /locus_tag="KRL67_002918"
     CDS             4052..4792
                     /locus_tag="KRL67_002918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate oxidoreductase"
                     /protein_id="HBH1523784.1"
                     /translation="MATEKLTPSVVSIPNKFCPGCGHGIVNRIIAEVIEEHGYEKNHV
                     LTLGVGCVCNMNFSWNGDKMQTAHGRASSTAIGVKVALPDTLVMTYQGDGDAYVIGLS
                     ETLNTAYRNHNVTVFVINNNNFAMTGGQMSWTTMPGQVTTTSVNGRDIKATGSPLKVP
                     EMVANFFDVAYVARGSVHSPKEIINLKKYIKNAIEAQLNGEGYSLVEILAPCPTNWGV
                     SLEKSIKWMEEEIVPYYALGEFKQRDGE"
     gene            4795..5334
                     /locus_tag="KRL67_002919"
     CDS             4795..5334
                     /locus_tag="KRL67_002919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HBH1523785.1"
                     /translation="MEKELVFAGFGGQGVLTLGLIVAESALELGKQVTWMPAYGPTMR
                     GGKAYSVVKFSDESIGGPDMEEIDILVAMNKPSLDYINLVKEGGTVVINTSAIDENVT
                     LREDISVVKINCQELAQKVNNPKAANIVVLGALIAKTGLFEKELALKTMCDFFEEKGK
                     GKFNVQNEAAFMEGYNNAI"
     gene            5458..7557
                     /locus_tag="KRL67_002920"
     CDS             5458..7557
                     /locus_tag="KRL67_002920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HBH1523786.1"
                     /translation="MSLEKLIRPKSIAIVGVTDKLGFGRSAALSIVKSKETDRVYYVN
                     PKREELFGRKCYKTIQEVPEVVDCVVVCTPRNVVPSVLKDSGELGTKAAVVYASGFAE
                     EGTEEGTNLENQLIEISNTYDMKILGPNCMGLLNCIDKVNLWAGGSKWDLDTKKPGIG
                     IVGQSGFITAEIVSSDYFNISYGFSTGNGNIVTLEDAVDFLVDDNYASVIAIYLEGLK
                     NPQKFIDALKRAAQKRKPIIILKSGRSEKGAISAASHTGNLAGSSKAFESIFEKYGVI
                     SVENLEQFMCLAQAFSVLDGNLPTNSNFAAINFSGGENTICADLAEENGVELAEISSE
                     TKEEMKKYLPGFATPKNPLDATTALFHEKDMIVGLLHTFEKDDSVGFTMVGANIRDEE
                     NEMHETLCQAVSEARKQGLKKPVFAVPTLEGNRYLDYRTRLEDNQVPIMSSVGTTFTC
                     FNKMAKFIDYDYSKRTLEFKAVKKRESNNVVALSELDSKIEMKKYGVPVPGQGNAKSI
                     DELDEILKYIKYPVVLKINSSEILHKSDVGGVKIGIKNRDEAVDAYNEILTNVKKAKP
                     DANIDGILVQEMVESGIEIIIGITNDDQFGPMLLVGLGGVFVEVFKDTTLYPLPINHD
                     EAIMMLKKLKSFKLLNGYRGSKPCDIDALADMMVKLGKYAYENKDEVKEIDLNPVFVY
                     PKGKGVCAVDALIVKYK"
     gene            complement(7824..8339)
                     /locus_tag="KRL67_002921"
     CDS             complement(7824..8339)
                     /locus_tag="KRL67_002921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin"
                     /protein_id="HBH1523787.1"
                     /translation="MISAKMEKLLNEQINHELYSAYLYLSMSSYLEAEGLKGFSNWFY
                     VQYKEETDHAMFFYKYLHNVGGKVQLDAIPMPDADFTSAMDILERTLAHEKKVTALIN
                     NLAAVANSESDFRTSQFLLWFISEQAEEETNCEDNIKRVKLAGEGGLFFVDQEFANRT
                     YAAPTNPPVTI"
     gene            complement(8431..9642)
                     /locus_tag="KRL67_002922"
     CDS             complement(8431..9642)
                     /locus_tag="KRL67_002922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family transcriptional regulator"
                     /protein_id="HBH1523788.1"
                     /translation="MSNKQGSNIKDVKIKNKLIILKLLSTNTPMSRVDLAKSTGLTKM
                     TLSNLVTELINENMIREIESSTLNAHSSGRKPILLDISNISPCICGILIRRNICKVII
                     SDLKGNIVKQLSETYPKCLSNDVLKDIIKRMFTKIMKINKRDIIGIGISSIGPVDDIN
                     GTILNPPDFGNVSNLNIVDFIKEMSDLPIFLINDANSGALAEKMYGLGKNISNYIYLH
                     IMNGIGAGLVLENKLHTGNLGQSGEIGHTSINFSGPLCSCGNNGCLDYYTSASNLIKR
                     IESLSHIYPNSPLINCKDFSLVKLIDEANNKDSLAMFLLDEFCTYVSYALVNTLTLID
                     CSSIIIGYDFNIPGTFIENTLLKKLTASASFSKYKKISVRHSNFGANAPLIGSIAIVS
                     YNFFNGSINFY"
BASE COUNT         3767 a         1243 c         1855 g         3168 t
ORIGIN      
        1 aaaatatact cctgaatcga gaatgagata tttaagattc aaaatatata ttattatacg
       61 tgaataataa aaagtaataa aattttataa gggaggggta gatgtgagta gtcaagaaag
      121 caaaccaaaa ggaaatctta tatcaaattt tagagatgat atgagaaacc ctaagaaaag
      181 agcaatatac acatattcaa gttctataaa cttagcgtac aattttatag gttactttgt
      241 atcaactgtt acaaatatct ttttaactga tgtggcaaaa cttggtgtaa ttatggcagg
      301 tggtatgcaa attatacaat ctttcataaa aattgctgta gctcctataa caggtacagt
      361 atttgacaaa caaccattca aaaaaggaaa gtattatcca tggcttagat atgcaccagc
      421 agcattagca ttaacatatg gaatattttt catagttcca ttaatgaaaa taagtccagc
      481 attgctttta cctttgatga cagctttatt attaatagca tcttgtgctc agcaagtagt
      541 ttttactata atttatgcta tttaccctgt agttgctaag acacctagag atagagttat
      601 aggctcaact actacgaata tattcaaaga agttggtaaa ttcttagtag gtttttcata
      661 tccgttatta ttagttttat ttgcagatgt attaggtgga gaaggaatgg gttatttagg
      721 tacctattta ttctttgcag caggatgcct tttagtattt tggttttctt caacagagat
      781 aataaaatct ggagcagaag aagaagttct tcaaaaagaa gagaagccaa agattaaagc
      841 aagtgaaatg gcaaaagctt tattcactaa tgtaccactt atgatggcat tttgtttaga
      901 atttcttata tgtataagat ctataggatt aggaccttta gctccatatt attttaaata
      961 tgtaatagaa gacgaacgtg gattagcagt atttttatca atcatgcctt tggtaagtgt
     1021 agcattcatg tttttcgcac cagtgtttat aaaaatctgt agagagcaaa aactagcttc
     1081 aataatttct ttttctatat gtgcgttatg ccacttattg gttgcatgga ctccttgggg
     1141 gaaaactact ttaggagtaa ctatgttatt ggcaattggt ggaggttttt caaatgtagt
     1201 atcaataata aatcttaatt tctttgccgg ttcttgtgat tatgggcatt ggaaatcagg
     1261 aaaagactta cctggtttaa gtatgtcact atatccagtt gcaatacaag taggagtatt
     1321 acttgcaact actataagaa cagttttaat gaactcaatg ggatatcaag ctgatatggt
     1381 agtcacagaa gcagttaaat caggttttat aaatatgatt tcttattcta tggcaatacc
     1441 tttaataata gccgttgtta tagcaatact atatccagta agtgacaaga aattaaacca
     1501 aataagagaa gagttaaatc gaagaaatgc ttagttaaaa gaaagttaag tagatggtta
     1561 atttaagcta ttagagtaag tagacgcaat agcgtttata taaatgaaaa tacatataca
     1621 gagggggctt ttaaaaatga tagaaaaatt attgagaaat actgtaaaag aattacatca
     1681 atatgttcca ggtgaaccaa tagaaaaagt taaggaaaag tatggtgtaa aagaaataat
     1741 aaaattagct tcaaatgaaa atcctctagg accatcacca aaagcaatgg aaactatgat
     1801 tgaaatgtta aaacaaggtc aactttatcc agagccagaa gctaacgaac ttagaagaaa
     1861 attagctgaa aagctagatt taaaaccaga aaattttatt gttgctaatg gtgcagataa
     1921 cgttatcact ctaataggtg aggcttttat aaatagagga gatgaagtta tatattgtaa
     1981 cccaacattt ccatcatata gaactgcaac tataaaaaat gaagggatac cagtagaagt
     2041 acctttaaca gaagattata agtatgattt acaaggtata ttagatagga ttacagataa
     2101 aacaaaatta atatgtgtat gtaaccctaa taatccaaca ggaacaattg tagatgataa
     2161 agagttagaa gaatttttaa agaaagtccc tgaaaatatt ataactatat tggatgaagc
     2221 atatatagaa tttttaacag ttcctaatta tgtggatgga cttaagtatg taagggaaaa
     2281 ttacaacgtt atagtaacaa gaactttctc taaaatatat ggtcttgcag ggcttagagt
     2341 aggatatggt attgctaaag atgagataat aagaacttta tttacagtaa aagagccttt
     2401 ctctgcaaat cgtgttgcta taagtggagc aacagcagca cttgatgatg aagagtttat
     2461 aaaagaaaca tatgaattaa atagagttgg aatggcttac tttaaagaag agtttacaaa
     2521 aatgggattc gatgttgtag attcacaatc aaatttcctt tatgtagata tgaaaacaga
     2581 tatacctaag ttatttgaag acttgaaaaa aagagggttt gtaataagac caagtgctac
     2641 acatgcacgt gtaagtattg gaactatgga agaaaataag aaatttgtag cagttttaaa
     2701 ggaaatatta aaggagagcc aagtattgga taatagatag aaatattatg aaacggggtg
     2761 tagatatgaa cttaaaaata aacgcagaga gatgtaaaag ttgtgaatac tgcgttatat
     2821 cttgtaaaaa aggagcatta aaaatatcaa gtaagataaa taaagaaggt tatgctcatg
     2881 tagaagttga tgaaagtaaa tgcatacttt gtggaatttg ctatcaagtg tgtccagata
     2941 atgtttttga aataattaaa gaagaagcat gtcaagaggc ttagaaggag ttgggataat
     3001 atgaaaaaga tgtggaaggg gaaccacgcc attgcagaag cagccctaag aggaggttgt
     3061 gaattttatg caggatatcc aattacacca caaacagagg taatggaatt tttatcacat
     3121 agaatgtctg aacttggaag aacttttata caatccgaaa atgagatggc tgctatatat
     3181 atggtatatg gagcatatgc aagtggaatg aggtctatga cgagttcttc aggaccagga
     3241 atatctttaa aacaagaagg gatttcttac ttatgtgcaa atcattaccc ttgtgtaatc
     3301 gtaaatgtac agagatgggg gcctggtctt ggttcattgg actcagcaca gacagactat
     3361 ctaagagata ctagaggggg aggaaatgga gattatcact tgatagttta tgcaccaaac
     3421 agtattcaag agactgtaga tttgatgtat aactcatttg atatagcgga gaagtacaga
     3481 gtacctgttg aaattttgac agaagcagct ttaggtcaaa tgatggaacc agtagaattt
     3541 ccagagttta agaaaagaga agacgattta ggttggactt atgatggaag taatagagac
     3601 catgcaaagg ttgcagataa ccaaaagcca actttttgta tggagaaacg tataagaata
     3661 actgaaaatg aacaacaatg ggaagattat caagtagaag atgcagaata tgtgtttgtg
     3721 gcgttcggca taccatcaag aacaactatg aatgctgtta gaagacttag agaacaaggt
     3781 gaaaaagttg gaataataag accaataaca gtgtggcctt tcccatataa agcatttgaa
     3841 aaggtaaaca aggatgtaaa aggatttatt tcagtagaat caacagatac aggtcagtta
     3901 gtagaagatg ttgctttagc ctcaaagaaa gtatgtaaag aaaatgtgcc tgtgtatggt
     3961 ctatttagtg gaaatcatat accaaaaaca ttgcaggtaa tggacactta tagcaaaata
     4021 aaatctggcg aaatcaagga ggtgttctaa tatggctaca gaaaaattaa cacctagcgt
     4081 tgtttcaata ccaaacaagt tttgcccagg ttgtggtcat ggaatagtaa atcgtataat
     4141 agcagaggta atagaagaac atggatatga aaaaaatcat gtattaacat taggagttgg
     4201 ttgtgtctgc aatatgaatt ttagctggaa tggtgataag atgcaaactg cgcatgggag
     4261 agcttcatct acagctatag gagttaaagt agcacttcca gatacattag tgatgactta
     4321 ccaaggtgat ggagatgctt atgttatagg tctttctgaa actttaaata cagcatatag
     4381 aaatcataat gtgactgtat ttgtaataaa caataataat tttgcaatga ctggtggaca
     4441 aatgtcatgg accactatgc cgggtcaagt cacaacaaca agtgtaaatg gaagagatat
     4501 aaaggctact ggtagtccac tgaaagttcc tgagatggtg gcaaacttct ttgatgtagc
     4561 atatgtagca agaggttcag ttcatagccc aaaggaaata attaatctaa aaaaatatat
     4621 aaaaaatgca atagaagctc aattaaatgg agaggggtat tccttagttg aaatattagc
     4681 accttgccca actaactggg gtgtatcact agagaaatct atcaaatgga tggaagaaga
     4741 aatagtgcca tattatgctc ttggcgaatt taagcaaagg gatggtgaat agttatggaa
     4801 aaagagctag tatttgcagg gtttggtggt caaggagttt taactcttgg tctgattgta
     4861 gctgaatcag cacttgaatt ggggaaacaa gtaacttgga tgcctgcata tggtccaact
     4921 atgagaggtg gaaaagccta tagtgtagta aaattttctg atgagagtat aggtggacct
     4981 gatatggaag aaatagatat tttagtagca atgaataaac catctttaga ctatataaat
     5041 ttagttaaag aaggtggaac agtagttatt aatacatctg caatagatga gaatgtaaca
     5101 ttaagagaag atataagtgt agttaaaata aattgtcagg aattagctca aaaagtaaac
     5161 aatccaaaag cagcaaatat agtagtactt ggagcattaa ttgctaaaac ggggttattt
     5221 gaaaaagaat tagctttaaa aactatgtgt gatttctttg aggaaaaagg aaaaggaaag
     5281 tttaatgttc aaaatgaagc agcattcatg gaaggctata ataacgccat ttgataaatt
     5341 acccttatct taagtgattt atctcaagat ttaataatct ttagtgccaa gagaaacttc
     5401 atcttgagat aaaattcact taaaatcaca actataaaca tataggagcg tgaatttatg
     5461 agtttggaaa aacttattag accaaaaagt attgctatag taggagtgac tgataaactt
     5521 ggttttggaa gaagtgcagc acttagtata gtaaaaagta aggagacaga tagagtatat
     5581 tatgttaatc ccaaaagaga agaattattt ggaagaaaat gttacaagac gatacaagaa
     5641 gttccagaag tagtagactg tgtagtagta tgtacaccta gaaatgtagt tccatctgta
     5701 ttaaaagatt ctggagagtt aggaacaaag gcagcggtgg tatatgctag tggatttgca
     5761 gaggaaggta cagaagaagg tacaaattta gaaaatcaac ttatagaaat atcaaataca
     5821 tacgatatga aaatattggg tccaaactgc atgggattgc taaattgtat agacaaagtt
     5881 aatctgtggg caggaggttc taaatgggat ttagatacta aaaaacctgg tataggaatt
     5941 gtaggtcaaa gtggatttat aactgctgaa attgtctcaa gtgattattt taatatatct
     6001 tatggttttt caacaggaaa tggaaatata gttacactag aagatgcagt agatttcctt
     6061 gtagatgata attatgcatc agtaatagca atttaccttg aaggtttaaa aaatcctcaa
     6121 aaatttatag atgctttaaa aagagcagca caaaaaagaa agccaataat aatattgaaa
     6181 tcaggaaggt cagaaaaagg tgctatttct gctgcttctc atacaggaaa tttagctggt
     6241 tcgagtaaag cttttgaaag tatttttgaa aaatatggag taatttctgt ggaaaattta
     6301 gaacagttta tgtgcttagc acaagctttt agtgttctag atggtaattt acctacaaat
     6361 tctaattttg cagcaataaa cttctcagga ggagaaaata ctatttgtgc agatttagca
     6421 gaagaaaatg gtgtagaact tgcagaaata tcttctgaaa ctaaagaaga aatgaaaaaa
     6481 tatcttccag gatttgcaac acctaaaaat ccactagatg ctacaacagc attgttccac
     6541 gaaaaggata tgatagtagg tctattacat acttttgaaa aggatgatag tgttggattt
     6601 actatggtag gtgcaaatat aagagatgaa gaaaatgaaa tgcatgaaac actttgtcaa
     6661 gctgtaagtg aagctagaaa acaaggattg aaaaaacctg tgtttgcagt tcctacactt
     6721 gaaggaaata gatatttaga ttataggact agattagaag ataatcaagt gcctataatg
     6781 tcatcagttg gaactacatt tacttgtttt aataaaatgg ctaagtttat agattatgat
     6841 tattctaaga gaactttgga atttaaagct gttaagaaaa gagagtcaaa taatgtagta
     6901 gcattatcag aattagatag taagattgag atgaaaaaat atggagtacc agttccaggg
     6961 caaggtaatg caaaaagtat tgatgaatta gacgaaatct taaaatatat taaatatcct
     7021 gttgttttaa aaataaattc ttctgaaata ttacacaaat ctgatgttgg aggagttaaa
     7081 ataggaatta agaatagaga tgaggcagta gatgcatata atgagatttt aactaatgta
     7141 aaaaaagcaa aaccagatgc taatattgat ggtatactag ttcaagaaat ggtagaaagt
     7201 ggtatagaga ttattattgg tataactaat gatgaccaat ttggaccaat gttattagtc
     7261 ggtttaggag gagtatttgt agaggtattt aaagatacta cattatatcc attacctata
     7321 aatcatgatg aagctattat gatgcttaaa aaactaaaat catttaagct tttaaatggc
     7381 tatagaggaa gtaagccttg cgatattgat gctcttgcag atatgatggt caaattagga
     7441 aaatatgctt atgaaaataa ggatgaagtc aaggaaatag atttaaatcc agtgtttgta
     7501 tatcctaaag gaaaaggcgt atgtgcagta gatgccttaa tagttaaata taaatagttt
     7561 ataagttttg gtattcaacc ataattaaag caccgactga aaatcacatt ttgtaagtta
     7621 gatagtgtga ttttcggtca ccccaaattt aatttaaaaa tattttaaag tgaaagtgta
     7681 gttttaagtt tcattgtgaa atagtactgt ataaacaata aaatactatg tataaaatga
     7741 ataataaaag gtgttttaat gataagaatc aatcttgatt ttcattaaaa caccttcttt
     7801 attatattaa aaatttttaa gcgttatatt gtcacaggtg ggtttgttgg agctgcatat
     7861 gtacggttag caaattcttg gtcaacaaag aataaaccac cctctccagc aagtttaaca
     7921 cgttttatat tgtcttcaca atttgtttct tcttctgctt gttcagatat aaaccaaagt
     7981 aagaattgag aagttctaaa gtctgattca gagttggcta cagctgctaa gttgtttatt
     8041 aaagctgtaa cctttttttc atgagctaac gtacgctcaa gaatatccat tgcagaagta
     8101 aagtctgcat ctggcattgg gatagcatca agctgaacct ttcctcctac attatgtaag
     8161 tatttgtaaa aaaacatagc gtggtctgtc tcttctttat attgaacata aaaccaatta
     8221 gaaaatcctt taagtccttc tgcttcaagg taagaagaca tagaaagata aagatacgca
     8281 gaatatagtt catggttaat ctgctcatta agtaattttt ccattttagc tgaaatcatt
     8341 tttatacctc caaattttaa atgctcttat aaaatataat aatattaaat ggaaaaatat
     8401 tcaataaaag tgtttaaata ttttacatat ttaataaaaa tttatactac cattaaaaaa
     8461 attataactt acaatggcta tagagccgat taatggtgca tttgctccaa aatttgaatg
     8521 tcttacactt atttttttat atttagaaaa acttgctgaa gcagtcaatt tttttaataa
     8581 tgtattttct ataaatgtac caggtatatt aaagtcatag cctattatta tagaactaca
     8641 atcaatcaat gtaagtgtat taactaatgc atatgatacg tatgtacaaa attcatcaag
     8701 caaaaacatg gcaagactat ctttattatt tgcctcatct attaatttga ctaaagaaaa
     8761 atctttacag ttaattaatg gggagttagg ataaatatga ctcaatgatt caattctttt
     8821 aattagatta ctggcacttg tataataatc taaacatcca ttgttaccac aactgcacaa
     8881 tggtccacta aaattaattg atgtatgtcc tatttctccg ctttgtccta ggttgccagt
     8941 atgaagctta ttttctagaa ctagaccagc tccgatacca ttcattatgt gtaggtatat
     9001 ataatttgat atatttttac ctaaaccata cattttttct gctaatgcac ccgaattagc
     9061 atcgtttatc aaaaatattg gcaaatcaga catttctttt ataaaatcta ctatatttag
     9121 gtttgataca tttccaaagt ctgggggatt tagtatggta ccatttatat catctactgg
     9181 acctattgaa gaaataccaa ttccaattat atctctcttg ttgattttca ttattttagt
     9241 aaacattctc tttattatat cttttaaaac atcatttgat agacatttag ggtaagtttc
     9301 agaaagttgt tttacaatat taccttttaa atctgaaata attaccttac agatatttct
     9361 tcttattagg attccacaaa tacaaggcga tatattagaa atatctaata aaataggttt
     9421 acgacctgat gaatgtgcat ttaaagttga tgactctatt tccctaatca tgttttcatt
     9481 aataagttca gtaactagat ttgataaagt cattttagta agtcctgtag acttagctaa
     9541 atcaactctg gacataggtg tatttgtaga aagtaatttc aatattatta acttattttt
     9601 aatttttaca tctttgatgt tagaaccttg tttgttactc atcgtaaatc tccttaaata
     9661 aagttaagtg ttttatttat tttcaagtat actatcacct tataatagag taacattttt
     9721 tacttgaaaa tatcattaat tagaatatat cacattatat atcataaaac atataaaaaa
     9781 atgttagaat taaattaaaa gtgaaaagta gattgatata taaatagtaa acacttaatt
     9841 aaaaaaagat aatgatagca ataattattg ttaccaagaa tctactactt caagtgttac
     9901 cttaggtatt taagttgata atggtttgca cataataaac ttgatgtaat atatcttctt
     9961 aataatatcg ataaaagatt tacttaaaaa taatatagat tatgtaaaca tataatctat
    10021 ttactgtata caa
//
LOCUS       DAEPXF010000143        10028 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain HB27
            SAMN13376389-rid12479423.denovo.150, whole genome shotgun sequence.
ACCESSION   DAEPXF010000143 DAEPXF010000000
VERSION     DAEPXF010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13376389
            Sequence Read Archive: SRR10518432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10028)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10028)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001069624.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001069624.1
            Genome Coverage       :: 315x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/15/2021 01:54:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,165
            CDSs (total)                      :: 4,076
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 89
            rRNAs                             :: 10, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 10 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 19 of 69
            Pseudo Genes (incomplete)         :: 44 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 10 of 69
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10028
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13376389-rid12479423.denovo.150"
                     /strain="HB27"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="China: Hebei"
                     /collection_date="2017"
     gene            270..1430
                     /locus_tag="KRL67_002923"
     CDS             270..1430
                     /locus_tag="KRL67_002923"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HBH1523789.1"
                     /translation="MKKEMLAMILAGGQGSRLGVFTKRIAKPAVSFGGKYRIIDFVLS
                     NCSNSGIDTVGVLTQYRPLILNSHIGMGSHWDLDRINGGVYVLQPFMNEKEGNWYNGT
                     AHAIYQNMDFVDTYNPEYVLILSGDHIYKMDYSKMLKFHKEKGSKATIAVIEVPWDEA
                     SRFGIMNTNEDSSIYEFEEKPSEPKSNLASMGVYIFDWKMLRNYFKEAEKNAEINYDD
                     FGKNLIPKMLEDNVGMYAYPFKGYWRDVGTIQSLWDANMDIIKSPETLDLADPKWKIY
                     TNTMAMPPQYIGKNANVHRSMIADGCRILGEVGNSVLSHGVVVGKGSKVIDSVIMPNV
                     VIGENVTIEKAMIGECATINDNVQIKNVNNEINVVSEYENIEPRCVLIEGGL"
     gene            1432..2562
                     /gene="glgD"
                     /locus_tag="KRL67_002924"
     CDS             1432..2562
                     /gene="glgD"
                     /locus_tag="KRL67_002924"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase subunit
                     GlgD"
                     /protein_id="HBH1523790.1"
                     /translation="MRNECLGIINLNKKGDPAINKLNYGRPIASTPIAGRYRIIDFAL
                     SNMINSGITKVGIFAKEKYRSLTDHIGSGKDWDLSRKKGGLSIFSPENTKYRNTYSHR
                     EGDIYTILANLDYIEKSEEEYILIAPSYMLCNLNYSQALEYHKKSHNDITIIYKNVNN
                     ANKDFAGNLTLNLDSNNRVINVGNNLGKFPRANICMETYIMKREDFVECIYNIVNKGS
                     YCYLEEFIIEEAENMKIGAYEYTGYLKCVNSVESYFEMSKDLLNLEVADELLYSERKI
                     FTKEKNESPTIYTDSAKVENSFIASGCLIEGTVKDSIIFRKVNVEKGTVIENSIVMQN
                     CVIKSNAKLYNAILDKNTSVSNGKELRGDEKYPLVVEKNTNI"
     gene            2591..4033
                     /gene="glgA"
                     /locus_tag="KRL67_002925"
     CDS             2591..4033
                     /gene="glgA"
                     /locus_tag="KRL67_002925"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011053260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase GlgA"
                     /protein_id="HBH1523791.1"
                     /translation="MKVFYVTAECWPFAKTGGLGDVSYALPKELKKEGVDVRVIMPKY
                     STIPSYLKDQLKEIAVFSVRVGWRNQYCGLLEMELDGVKFYFIDNEFYFRREDERKSI
                     YGYGDDAERYTFFTDAVLEAISRIDFYPDVIHINDWHTGMLPLILKERYATLEGYKNI
                     KTMYTIHNLQYQGVFDKHVLYDILDLPQTYFDNGDIEYYGSINFMKAGINFADKIITV
                     SPTYANEIQTSFYGEQLDGLLRKESGKLKGILNGIDYDLNDPAKDKDIFVHYDVDSID
                     KKVENKLRLQDILGLKKDSSIPLIGIVSRLVSQKGFDLIAYMMPELMREDLQIVVLGT
                     GEHQYQSMFNYYDSNFSDKVSARITFNASLAQQIYAASDMFLMPSLFEPCGIGQMLAM
                     RYGSLPIVRETGGLRDTVTPYNKFTGEGNGFSFKNYNAHEMFFCLKNAIKVFKDKEKW
                     IKLVENAMKTDNSWKKSAKEYIETYRDICD"
     gene            4047..6467
                     /locus_tag="KRL67_002926"
     CDS             4047..6467
                     /locus_tag="KRL67_002926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch/alpha-glucan phosphorylase"
                     /protein_id="HBH1523792.1"
                     /translation="MVTISKKKFKQKFEVKMYSLYAQSIKEATDEQLLNVLCSLLKDE
                     IAKKWVATKLDKKKEVYYFSLEFLIGRQLKSNLLNLNIEEEVREGLSELGINLDDLIE
                     AEVDPALGNGGLGRLAACFLDSMASLNISGQGYGIRYKYGLFEQKFVNGYQVEVPDNW
                     LTEGRYAWETVRPNEATMVKFGGEVELIKEGSHLKVIHKNYLPVMAMPYDIPIIGYQN
                     QCINTLRLFKSEIPKRDFGELTSNALNYSGSYEEALKHKYYTEEISQVLYPDDSNYAG
                     KLLRLKQEYFFVSAGIQDIIRKYKKNKLNINNLFDKVAIHINDTHPTLCIAELMRILL
                     DEENLSWDEAWEITKKTVSYTNHTIMSEAMEKWPVSMMKELLPRIYMIIEEINRRYVE
                     ELNNKGYDQNRIKRMSIIDCDNINMANLCIVTSHSVNGVAKLHTHILETEVLKDFYQD
                     EPNKFNNKTNGIAHRRWLISSNPQLSNLITDLIGDSWKKDTLQLKNIEKFKNDSSVLQ
                     RLDDIKYNNKANLAKFIKDKYDLNVDPSSIFDVQVKRLHAYKRQLLNIFNVLHMYHEL
                     LDNPNLNLDPRTFIFGAKAAPGYYLAKCIIKFINSVASTINNDVRVKDKLKVVFLENY
                     GVSLAEIIIPAANVSEQISTTTKEASGTSNMKFMMNGAITLATLDGANVEICEQVGKE
                     NMFLFGLSAEQVLNYNKYGGYSSLDLYHSNMDIKRVVDDLINGFIPNLGEEGRSIYNS
                     LTTYNDEYFVLRDFENYGQAQADINRLYRDKEKWNKMSLVNIANSGFFSSDRTISEYA
                     KDIWFKRV"
     gene            6472..8337
                     /locus_tag="KRL67_002927"
     CDS             6472..8337
                     /locus_tag="KRL67_002927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 13 protein"
                     /protein_id="HBH1523793.1"
                     /translation="MQSVIEYNSWDKNFKAPFGALKFDEELNICVKVNEGYNVKSIFL
                     EINREEEMRTITLNEELDNDKLGKCFCGKIEKFDGTGVYFYYFKVDVEMDGQIKTLFY
                     GKNRDNGYSCEYNYSDINKYQITVYKDFKVPTWYKEGILYHIFVDRFNNGNRSGKVDN
                     PKKNSFIYGNWEDIPMYIKDSQGDVIRWDFHGGNLRGIINKLGYLKKLGVSILYLSPI
                     FEASSNHKYDTGDYKKIDPMFGDEDTFKELIDKAKEKGISIVLDGVFSHTGADSKYFN
                     MYGNYNSLGAYQSKESPYYSWYMFEEFPQTYKSWWDVKTLPNINELEHSYMDYIIYDK
                     DSVINKWINMGIKGWRLDVADELPTKFIRELKKELKKADDDSILIGEVWEDASNKISY
                     GQRRSYLLGEELDSVMGYPFRNNMFSFLKGEINSYELCNRYMQIKENYPKESFKSNLN
                     LIGTHDVTRAKTELNDDVDLVKLAVAIQMTFEGVPYIYYGDEAGLCGGVDPDNRRTYP
                     WKNEDEDMLNFYKDVIKIRNKNKLLSSGNTEFIYTNNDSVFSFIRVNENNDRILILVN
                     RSENVESISLCMESSFIEEIPIKYSLKNVNSTIQIENNELKVDLDSKSFRIFRVN"
     gene            8582..9235
                     /locus_tag="KRL67_002928"
     CDS             8582..9235
                     /locus_tag="KRL67_002928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBH1523794.1"
                     /translation="MKVDPLTTQVYDYISKKIQNGEYEANQRITESEICKSIGVSRTP
                     AREALTRLAGENLLEKIPNKGFVVKEFQEKEKLDTYSVIGVLDALAGSSALQNLTESD
                     LVKMEELTEMMAVSIKYKNYNSYLKLSNEFHDIYITKSDNQVLVNLLNSLRYNFMSKS
                     YTSDNEDELYKMLTYSNNQHIEVVKFMRENDLENVERVLREHWKTIPIAEMKKEKLL"
     gene            9331..9930
                     /locus_tag="KRL67_002929"
     CDS             9331..9930
                     /locus_tag="KRL67_002929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HBH1523795.1"
                     /translation="MYPNKETAIEELEVAGKMNPGPWIKHSYNVANVAKLIAEHCENL
                     NSEKAFVCGLLHDIGRRTGISSVRHIIDGYDYVISKGWDEVARVCLTHSFPVKDIEAD
                     IGNKDISKKQYDFINHYLNNLEYNDYDKLIILCDALADANGFCILEKRFVDTTRRYGI
                     YPFSVERWNKTYEYKEYFEKIINKSIYTLLPNIEKSIYY"
BASE COUNT         4005 a          988 c         1850 g         3185 t
ORIGIN      
        1 gtttttatat ttataaaata aaatttgtat tattttttaa ccgataattg aatattaata
       61 aatactatag tgcttatgga aaataatgta tacgttggta agtccattat ggggtataca
      121 ttatatcaat tgtatagttg atattatttt atttttatta aggtattatt taagcaacat
      181 ttatacacga catttattga ctgataaagt ttataaaagt aaaaatgcaa ttataaatat
      241 taattactag atgaggggag tgtcattaaa tgaaaaaaga gatgttagct atgattttgg
      301 caggagggca aggttcaaga ttaggtgttt ttacaaaaag aattgccaaa ccagctgtat
      361 catttggagg taagtataga attatagatt ttgtgttaag taattgttca aactctggta
      421 ttgatactgt aggtgtttta actcaatata gaccgttgat attaaattca catataggca
      481 tgggaagtca ttgggatttg gacagaataa atggtggtgt ctatgtttta caaccattta
      541 tgaatgaaaa agaaggaaat tggtataatg gtacagcaca tgcgatatat caaaatatgg
      601 actttgtaga tacctataat ccagaatatg tgttgatatt atctggtgac catatatata
      661 agatggatta tagcaaaatg ttaaaatttc ataaggaaaa aggctctaaa gctacaatag
      721 cagtgattga agttccttgg gatgaagctt caagatttgg gataatgaat acaaatgaag
      781 attcaagtat ctatgaattt gaagaaaaac caagtgaacc taagagcaat ttggcatcta
      841 tgggagtata catatttgat tggaaaatgt tgagaaatta ttttaaggaa gcagaaaaaa
      901 atgcagaaat aaattatgat gattttggaa aaaatttaat acctaaaatg ttagaagata
      961 atgttggaat gtatgcttat ccatttaaag gatactggag agatgtagga actatacaaa
     1021 gtctatggga tgctaatatg gatataataa aatctccaga aacacttgat ttagcagacc
     1081 ctaagtggaa gatatataca aatactatgg caatgcctcc acaatatata ggtaaaaatg
     1141 caaatgttca tagatcaatg atagctgatg gttgtagaat tttaggtgaa gtaggtaact
     1201 ctgtattatc acatggagtt gttgttggta aaggaagtaa agtaattgat tcagtaataa
     1261 tgccaaatgt tgtaataggt gaaaatgtaa ctatagaaaa agctatgata ggtgagtgtg
     1321 caactataaa tgataatgtt cagataaaga atgtaaataa tgaaataaat gtagtttctg
     1381 agtatgaaaa tatcgaacct agatgcgtat taatagaagg aggtctttaa gatgagaaat
     1441 gaatgtttag ggataataaa tttaaacaaa aaaggagacc cagcaataaa caaacttaac
     1501 tatggaagac caatagcatc tacaccaata gctggaagat atagaataat agactttgct
     1561 ttatctaata tgataaattc aggaatcact aaagttggaa tattcgcaaa ggaaaaatac
     1621 agatcattga cagaccatat aggtagtggt aaagattggg atttaagtag aaaaaaaggt
     1681 ggcctatcaa tttttagtcc agaaaacact aagtatagaa atacttactc acatagagaa
     1741 ggtgatatat atactatctt agctaatcta gattatatag aaaagagtga agaagaatat
     1801 attttaatag ctccaagcta tatgctatgt aatttaaatt attctcaagc attggaatac
     1861 cataaaaaat ctcataatga tataactata atatataaaa atgtaaataa tgcaaataaa
     1921 gattttgctg gtaacttaac tttaaattta gatagtaaca atagggtaat aaatgtagga
     1981 aataatttag gcaaatttcc tagagcaaac atatgtatgg agacatatat tatgaagcgt
     2041 gaagattttg ttgaatgtat atataacatt gtaaataaag gcagttattg ttatttagaa
     2101 gaatttatta ttgaagaagc agaaaatatg aaaataggag cctatgaata tacaggatat
     2161 ctaaaatgtg taaattctgt tgaatcatat tttgaaatga gcaaagattt attaaattta
     2221 gaagttgcag atgaattatt atattctgaa agaaagatat ttacaaaaga aaagaatgaa
     2281 tcacctacaa tttatacaga tagtgctaaa gtagaaaata gctttatagc ctctggttgt
     2341 ttgattgaag gtactgtaaa agatagtatc atatttagaa aggtaaatgt tgaaaaagga
     2401 acagttatag aaaactcaat agtaatgcaa aattgtgtaa taaagtcaaa tgcaaaacta
     2461 tataatgcaa tattagataa aaatacgagt gtatcaaatg ggaaagagtt aagaggagat
     2521 gaaaaatatc ctcttgtagt tgaaaagaat acaaatatat aaataaattt tttagaaggg
     2581 ggaatgacta atgaaggttt tttatgtaac agcagagtgt tggccctttg caaaaactgg
     2641 aggattagga gatgtttctt atgcattgcc taaagaatta aaaaaagaag gtgtagatgt
     2701 aagggtaata atgcccaaat attcaactat accaagttat ttaaaagatc aactaaaaga
     2761 aatagctgtg ttcagtgtac gtgttggatg gagaaatcaa tactgtggtc ttttagaaat
     2821 ggaattggat ggtgtaaaat tttactttat agataatgaa ttttatttta gaagagaaga
     2881 tgaaagaaag agtatctatg gatatggaga tgatgcagaa agatatactt tctttacaga
     2941 tgcagtttta gaagctataa gtagaattga tttttatcca gatgtaatac atataaatga
     3001 ttggcatacg gggatgttac cattaattct aaaagaaaga tatgcaaccc ttgaggggta
     3061 taaaaatata aaaactatgt atactataca taatctccaa tatcaaggtg tatttgataa
     3121 gcatgtttta tatgatatat tggacttacc acaaacatac tttgataatg gagatataga
     3181 gtattatggc tcaataaatt ttatgaaagc tggtataaat tttgcagata aaattataac
     3241 tgttagtcct acttatgcaa atgaaataca aacatctttt tatggtgaac aattagatgg
     3301 attacttaga aaagagtcag gaaaattaaa aggtatatta aatggtatag attatgattt
     3361 aaatgaccct gcaaaagata aagatatatt tgttcattat gatgtagact caattgataa
     3421 aaaagtagaa aataaattga gattacaaga tatattggga cttaaaaaag attcatctat
     3481 tccgttgata ggtatagttt cgagattagt ttctcaaaaa ggatttgatt taatagcata
     3541 catgatgcca gaacttatga gagaagattt acagattgtc gttttaggaa ctggagaaca
     3601 tcaatatcaa tcaatgttca actattacga ttctaatttt tcagataaag tatcagctag
     3661 aattactttc aatgcatcat tggctcaaca gatatatgct gcaagtgata tgttcttaat
     3721 gccatcatta tttgagcctt gtggtatagg tcaaatgtta gctatgagat atggttcttt
     3781 accaatagtt agggagactg gagggcttag ggatacagta acaccttata ataaatttac
     3841 tggagaaggc aatggattta gttttaaaaa ttacaatgct catgagatgt ttttctgttt
     3901 gaaaaatgca attaaagtgt ttaaggacaa agaaaaatgg attaaattag ttgaaaatgc
     3961 aatgaaaaca gataatagtt ggaaaaaatc agctaaggag tatatagaaa catatagaga
     4021 catatgtgat taaaggagtg gtttttttgg taacaataag caaaaagaaa ttcaagcaaa
     4081 aatttgaagt aaaaatgtat agcttatatg cacaatctat caaggaagct actgatgaac
     4141 aattgttaaa tgtattatgt agtctattga aagatgagat agcaaaaaaa tgggttgcta
     4201 caaagttaga taagaaaaaa gaagtttatt attttagttt agaattttta atagggagac
     4261 agttaaaatc taatttatta aatttaaata ttgaagaaga agttagagaa ggtttatctg
     4321 aattaggaat caacttggat gatttaattg aagctgaagt agacccagcc ttaggaaatg
     4381 gtggacttgg tagattagca gcttgctttt tagattctat ggcatctttg aacataagtg
     4441 gtcaaggata tggaataaga tataaatatg gtctgtttga acaaaaattt gtaaatggtt
     4501 atcaggtaga agtgcctgat aactggctta cagaaggacg atatgcatgg gaaactgtaa
     4561 gaccaaatga agcaacaatg gtgaagtttg gtggtgaggt agaattaatc aaggaaggta
     4621 gtcatttaaa agtaattcat aaaaactacc ttcctgtaat ggcaatgcct tatgatatac
     4681 caataatcgg ataccaaaac caatgtataa atactttaag attgtttaag agtgaaatac
     4741 caaagagaga ttttggtgaa cttacctcaa atgctctaaa ttattctggt agctatgaag
     4801 aggctttaaa acataagtat tatactgagg aaatatcaca ggtattatat cctgatgatt
     4861 caaactatgc aggtaagttg ttgaggttga aacaagaata cttttttgta agtgcaggga
     4921 tacaagatat aataagaaaa tataaaaaga acaaattaaa tattaataat ttattcgata
     4981 aggtagcaat tcatataaat gatacacatc ctaccttatg catagcagaa cttatgagaa
     5041 ttttgcttga tgaagaaaat ttatcttggg atgaagcatg ggaaataact aaaaaaacag
     5101 tatcctatac taatcatact ataatgtcag aagcaatgga aaaatggcct gtaagtatga
     5161 tgaaagaact attaccaaga atttatatga taatagaaga aataaataga agatatgtag
     5221 aggaattaaa taataaagga tatgaccaaa atagaataaa aagaatgagt ataatagact
     5281 gtgacaatat caatatggca aacttatgta ttgtcacaag tcatagtgta aatggagttg
     5341 ctaagcttca tactcatatt cttgaaacag aggtattaaa agatttctat caagatgaac
     5401 caaataaatt taataataag actaatggaa ttgcacatag gcgatggctt ataagttcaa
     5461 atcctcaact tagcaattta attacagatt taattggtga ttcatggaag aaggatacct
     5521 tacagttaaa aaatattgaa aaatttaaaa atgattcctc tgttttacaa agacttgatg
     5581 atataaaata caataataaa gcaaatctag caaagtttat aaaagataaa tacgatttaa
     5641 atgtagaccc aagttcgata tttgatgttc aagttaagag attgcatgca tataaaagac
     5701 agttactgaa tatatttaat gtacttcata tgtatcatga attacttgat aatcctaatt
     5761 tgaatcttga cccaagaacc tttatatttg gcgcaaaagc agcaccagga tattatttgg
     5821 ctaaatgtat aattaaattt ataaattctg ttgcaagtac tataaataat gatgttagag
     5881 taaaggacaa gttaaaagta gtatttttag agaattatgg agtatcatta gcagaaataa
     5941 taataccagc agcaaatgtt agtgagcaaa tttcaaccac aacaaaggaa gcatctggaa
     6001 caagtaatat gaagtttatg atgaatggtg ctataacttt ggctacactt gatggcgcaa
     6061 atgtagagat atgtgaacag gttggtaaag aaaatatgtt tttatttgga cttagtgcag
     6121 aacaagtact aaattataat aaatatggtg gatattcttc acttgaccta tatcattcta
     6181 atatggatat aaaaagggta gtagatgatt taataaatgg atttattcct aatcttggcg
     6241 aagaaggaag aagtatttat aactcactca ctacttacaa tgatgagtat tttgtattga
     6301 gagattttga aaattatggt caagctcaag cagatataaa caggttatat agagataaag
     6361 aaaaatggaa taaaatgtct ttagttaaca tagctaactc tggatttttt tcttctgata
     6421 gaacaatttc tgagtatgca aaagacattt ggtttaaaag ggtgtgatta aatgcaaagt
     6481 gtaatagaat ataactcttg ggataaaaat tttaaggctc cgtttggagc tttaaaattt
     6541 gatgaggaat tgaacatttg tgttaaagta aatgagggat acaatgtcaa gtctattttc
     6601 ctagaaataa atagagaaga agaaatgaga acaataactt taaatgaaga attagataat
     6661 gataaacttg gaaagtgctt ttgtggtaag attgaaaagt ttgatgggac aggtgtttat
     6721 ttttattact ttaaagttga tgttgaaatg gatggtcaga taaagacttt attttatgga
     6781 aaaaacagag acaacggata ctcttgtgaa tataattata gtgatattaa taaatatcaa
     6841 atcactgttt acaaagattt taaagtacca acatggtaca aagaaggaat actttatcat
     6901 atatttgtag atagattcaa taatggaaat cgtagtggta aggttgataa tcctaaaaaa
     6961 aatagtttca tatatggaaa ttgggaagat atacctatgt acataaagga tagtcaaggg
     7021 gatgttataa gatgggattt ccatggtgga aatttaagag ggataatcaa taaacttgga
     7081 tatttaaaaa aattaggtgt aagtatatta tatctaagcc ctatatttga agcctctagt
     7141 aatcataagt atgacacagg tgactataaa aaaattgacc caatgtttgg ggatgaagat
     7201 acatttaaag aacttattga taaagcaaaa gaaaaaggta tatcaatagt attagatgga
     7261 gtttttagtc atactggagc agatagtaaa tattttaata tgtatggaaa ctataatagt
     7321 ttaggagcct atcaatcaaa agaatctcca tattattcat ggtatatgtt tgaggaattt
     7381 cctcaaacat ataaaagttg gtgggatgtt aagacattac caaatataaa tgaattagaa
     7441 catagctata tggattatat aatatatgat aaggatagcg ttataaataa atggataaat
     7501 atgggtatta agggctggag actagatgtt gcagacgaac taccaactaa gtttataaga
     7561 gagttaaaaa aggaattaaa aaaggcagat gatgactcaa ttcttatagg tgaagtatgg
     7621 gaagatgcat caaataagat tagctatggt cagagaagaa gttatttatt aggtgaagaa
     7681 ttagattctg ttatgggtta tccttttaga aataacatgt tttctttctt aaaaggagaa
     7741 attaattcat acgaactttg taatagatat atgcagatta aagaaaatta tccaaaagaa
     7801 tcatttaagt ctaatttaaa tttaattgga acacatgatg ttacaagagc taaaacagaa
     7861 ttaaatgatg atgtagattt agtgaagctt gctgtagcta tacagatgac atttgaagga
     7921 gtaccatata tatactatgg agatgaagca ggtctttgtg gaggtgttga cccagataat
     7981 agaagaacat atccatggaa aaatgaagat gaagatatgc ttaattttta taaagatgtt
     8041 ataaaaatta gaaataagaa taaactttta agttctggaa atacagaatt tatatatact
     8101 aataatgatt ctgtattttc ctttattaga gttaatgaaa ataatgatag aatattaatt
     8161 ttagtaaata gaagtgaaaa tgtggaaagt atatcattat gtatggagag tagttttatt
     8221 gaggaaatac ctataaagta tagtcttaaa aatgtaaata gtactataca aattgaaaat
     8281 aatgagttga aagtagattt agactctaaa tcatttagaa tttttagagt aaattaaata
     8341 tatttgttta gaaaaataaa tagaaaataa ggtgaatgtg gaaagagttt tgttttatca
     8401 aaactttttc tacttttttt gtataaatta tcataagaat tttagtattt caaaatatta
     8461 caaagaagta taaaaaatat cataattgtg atattatata aattagtaag ataaaactga
     8521 actctctacc aaagttgtag ggagatttaa cttttataat cttaatatgg aggaacgtct
     8581 tatgaaggta gatccactta caactcaagt atatgactat atatcaaaga aaatccaaaa
     8641 tggagagtat gaagctaatc agagaattac tgaatctgag atttgtaaat ctatcggagt
     8701 tagtagaaca ccagcgagag aagcattaac taggttagca ggtgaaaatt tactagaaaa
     8761 aatacctaat aagggatttg tagtaaaaga atttcaagaa aaggagaaat tagacaccta
     8821 ttctgtaatt ggagtattgg atgcgttagc cggttcttca gcattacaaa atttaactga
     8881 atcagaccta gtaaaaatgg aagaattaac agaaatgatg gcggtctcta taaagtataa
     8941 aaattacaat agctatttaa aattaagtaa tgaatttcac gatatataca taactaaatc
     9001 agataatcag gtgttagtaa atctattgaa ctctttaaga tataatttta tgtcaaagtc
     9061 atatactagt gataatgaag atgagttata taaaatgttg acatacagca acaatcagca
     9121 tattgaagta gtaaaattca tgagggagaa tgatttagaa aatgttgaaa gagttttgag
     9181 agaacattgg aaaacgatac ctatagcaga aatgaaaaaa gagaagcttt tataaagttt
     9241 ctctttattt tttataccat tttatcacta tttttttgaa ttattaagta gaaaataata
     9301 attaatttaa atttgaggag gaaaactaat atgtatccaa ataaagaaac tgcaatagaa
     9361 gagttagaag tggctggcaa aatgaatccc ggtccatgga taaaacattc ttacaatgtt
     9421 gctaatgtag ctaaattgat tgcagagcat tgtgaaaatt tgaatagcga aaaggctttt
     9481 gtgtgtggat tattacatga cattggcaga agaactggta tttcttctgt aaggcatatt
     9541 attgatggat atgattatgt tatttctaag ggatgggatg aagtggcaag ggtatgtttg
     9601 acacattctt ttcctgttaa ggatatagaa gctgatattg gaaataaaga tattagtaaa
     9661 aaacagtatg attttatcaa tcactattta aacaaccttg aatataacga ttatgataag
     9721 ttgattattc tttgtgatgc attagcagat gcaaatgggt tttgtatttt agaaaaaaga
     9781 tttgttgata caacacgaag atatggaata tatccgtttt cagtagaacg atggaataag
     9841 acttatgagt ataaagaata tttcgagaaa ataatcaata agtctatata tacattgtta
     9901 cctaatattg aaaaatctat atattattaa atcctaagtt aataacttaa tatccagtat
     9961 caccagcagt tggtcattat gattgactgc ttttattctg cttatattaa aaaaatacat
    10021 catggttt
//
LOCUS       DAEPXF010000144        10009 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain HB27
            SAMN13376389-rid12479423.denovo.151, whole genome shotgun sequence.
ACCESSION   DAEPXF010000144 DAEPXF010000000
VERSION     DAEPXF010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13376389
            Sequence Read Archive: SRR10518432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10009)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10009)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001069624.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001069624.1
            Genome Coverage       :: 315x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/15/2021 01:54:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,165
            CDSs (total)                      :: 4,076
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 89
            rRNAs                             :: 10, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 10 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 19 of 69
            Pseudo Genes (incomplete)         :: 44 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 10 of 69
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10009
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13376389-rid12479423.denovo.151"
                     /strain="HB27"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="China: Hebei"
                     /collection_date="2017"
     gene            complement(470..1627)
                     /locus_tag="KRL67_002930"
     CDS             complement(470..1627)
                     /locus_tag="KRL67_002930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HBH1523796.1"
                     /translation="MYLVISKKKAIFLGVILVIITAMVTSAFQLTLGNKVVISKELYE
                     DYKKYDKLLGLESIIKQDFYKKVSDTDLVNGASKGLFLGTNDKYSGYYTKDEMENLIN
                     DSEGSYVGVGMYIGASKDGGLVVVPMKDSPAEKAGVKSGDKLVKVNGKSVSYKNSDEA
                     VRMMKGKKGKTVELTILREDKQLNFKVKTDQIIEKSIESKVIDNDLGYIEITQFISST
                     YTDFDKALKELKTKNIKGLVIDLRNNPGGMLDICKEVADELIGEGTIVYTKDNKGNTE
                     YLKSDKEKLGLPIVVLTNGESASAAEILTAAIVDNKEGISVGTTTFGKGLVQSVVRLK
                     DGTGYKLTTAQYFTPNGDYINEKGIKPTIEEKDENKQLDVATKWLREQIDK"
     gene            1781..2350
                     /locus_tag="KRL67_002931"
     CDS             1781..2350
                     /locus_tag="KRL67_002931"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HBH1523797.1"
                     /translation="MKKEFRKKVIESRKNKSANFVLSNSNLITEKLLQMEDIRNATNI
                     MLYLNFNNEVQTDNLIKKLLSLKKIVSSPITIKENHTLIPTQITDLKEGLRIGAYGIR
                     EPNGKSPTIDIKALDVVIVPAVAYDIHCYRLGYGGGFYDRFLENLRKDAITIGIAFDF
                     QIFDSIPKEAHDAQLDYIVTETRIITPNN"
     gene            complement(2532..2948)
                     /locus_tag="KRL67_002932"
     CDS             complement(2532..2948)
                     /locus_tag="KRL67_002932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH1523798.1"
                     /translation="MNVKFNIKGIIYGVIALVFVIGGFIFIPSMFVEKSKPIDYTVLQ
                     RNSIPEKILDVMDKYTNEERALAAKIDNKIYIIVTRGNNKYGIEMDKIESVVEEGKDV
                     LRVKIKYKDKEDSFPYIVVETNMSELPDRIELNATK"
     gene            complement(3174..4268)
                     /locus_tag="KRL67_002933"
     CDS             complement(3174..4268)
                     /locus_tag="KRL67_002933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH1523799.1"
                     /translation="MSEKVNVKTVISFAGAYVATVIGSGFATGQEIMQFFSFYGFAGI
                     IGGIISMVLFSWMGASVMSKGKELQLKEPIKIYRVYCGKYLGMFFEWFGPLFLFGVFV
                     VMISGAGATLTEYYGLNPFVGRVGMAVVALLTVSLGLDRLSKILGGIGPIIIIFTLLV
                     GGISLAKNIGNIGEAAQVLSTVKIAKPVPNAYLSGVIYTTYNTIVVMAFLTGLGASAA
                     NKKEAICGGILGGVALMAAAIMMHLAILSDIGNLYSKAIPSLFLADKISPIIGVLFSI
                     ILILGIYTTAVPLLWSVTNRFVDDNHPKFKLITLIAAILGLIGGFLPFDKLVGILYPY
                     TGYMGVIILVCVLYRQITKTSGYQEGISDK"
     gene            complement(4691..5632)
                     /locus_tag="KRL67_002934"
     CDS             complement(4691..5632)
                     /locus_tag="KRL67_002934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase family protein"
                     /protein_id="HBH1523800.1"
                     /translation="MSKMATREAYGKALVKLGQINDNVVVLDADLSKSTKTHDFYKSF
                     PDRFFNMGIAEQNLIGAACGLSTAGKIPFASTFAMFATGRAFEIIRNSVCYPKLNVKI
                     CATHAGLTVGEDGASHESVEDIAIMRAIPNMTVLVPADGVETEKIIFEIAKYNGPVYV
                     RLGRSSVPVLFDEDYKFEIGKGTVLREGKDVSIIACGIMVNEALLAQEKLQEEGINAR
                     VINMSSIKPIDKDLILESAKETNAIVTVEEHSIIGGLGSAVSEVVGESCPTIVKKVGI
                     KDTFGESGTPNELLKKYELTCDDIVKTVKEAIIAKRM"
     gene            complement(5625..6452)
                     /locus_tag="KRL67_002935"
     CDS             complement(5625..6452)
                     /locus_tag="KRL67_002935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HBH1523801.1"
                     /translation="MRDHKGLNEIARIIRRDIVSMIHRAKSGHPGGSLSVVEILTALY
                     FDEMNVDSSNPKMEDRDRFVLSKGHAAPALYATLAEKGYFDKEELNGLRKIGRMLQGH
                     PDMKGTPGVEISTGSLGQGFSVACGMAMASKLDNAPWNVYILLGDGEVQEGIVWEAAM
                     SAAHYKLDNLIAFLDNNGLQIDGDIESVMSLGSIVDKFKAFGWNVIEIDGHDFDQIFA
                     ALDIAKSTVKKPTMIVAKTIKGKGISFMENQAGWHGTAPSDEELEKALLELGGADNE"
     gene            complement(6623..7006)
                     /locus_tag="KRL67_002936"
     CDS             complement(6623..7006)
                     /locus_tag="KRL67_002936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HBH1523802.1"
                     /translation="MKGRLPKVSTNCEIKRGDLYYADLSPVVGSEQGGVRPVLIIQND
                     VGNKYSPTVIVSAITSQINKAKLPTHVEISSNEYGLNKDSVILLEQIRTIDKKRLREK
                     IGCLDENMMIKVDDGLQISLGLFTF"
     gene            complement(6975..7262)
                     /locus_tag="KRL67_002937"
     CDS             complement(6975..7262)
                     /locus_tag="KRL67_002937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix protein, CopG family"
                     /protein_id="HBH1523803.1"
                     /translation="MYDKSKEKIEVTLPDSLISEVDSIVQMENSNREDFAKAAFQFYI
                     TQKKKIHIKESMKTGYREMGQINLSLAELGMTVEEVSSDDKNEGKIAQGEY"
     gene            complement(7295..8452)
                     /gene="alr"
                     /locus_tag="KRL67_002938"
     CDS             complement(7295..8452)
                     /gene="alr"
                     /locus_tag="KRL67_002938"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HBH1523804.1"
                     /translation="MQKITVPTWAEINLDNLRFNLNNIKNLLEEDIKICGVIKADAYG
                     HGAVEVAKLLEKEKVDYLAVARTAEGIELRQNGITLPILNLGYTPEEAFEDSIKNKIT
                     MTVYSLETAQKINEIAKSLGEKACVHVKIDSGMTRIGFQPNEESVQEIIELNKLEYID
                     LEGMFTHFATADEVSKEYTYKQANNYKFMSDKLDEAGVKIAIKHVSNSAAIMDCPDLR
                     LNMVRAGIILYGHYPSDDVFKDRLELRPAMKLKSKIGHIKQVEPGVGISYGLKYTTTD
                     KETIATVPIGYADGFTRIQKNPKVLIKGEVFDVVGRICMDQIMVRIDKDIDIKVGDEV
                     ILFGEGEVTAERIAKDLGTINYEVLCMISRRVDRVYMENNELVQINSYLLK"
     gene            complement(8467..9057)
                     /locus_tag="KRL67_002939"
     CDS             complement(8467..9057)
                     /locus_tag="KRL67_002939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH1523805.1"
                     /translation="MRKKWTIVCIMFLALVIIVIGCQKRQSTKEEVYKDFQKQISDMN
                     YYSCKAEVEVVGNKSPHNYVLIHTYKKTDNYKLEVISPKHLKGKSIEYQGDKILVKNP
                     KISDVVELPNTGKNNQYLFVGDFIKNYLQNEEMKVKLSKGHLVLETFIPGDNKYFNKQ
                     VLYVNADTKNPEKMEVLDKEGVPRFTVKYKDFEYRN"
     gene            complement(9078..9539)
                     /locus_tag="KRL67_002940"
     CDS             complement(9078..9539)
                     /locus_tag="KRL67_002940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HBH1523806.1"
                     /translation="MMDKTAKEIMTTDVIVAKQDDSIADVANMLIAEKIGGLPVVDSE
                     NRVVGIISETDILKKEKYIEAPLYINLLQGLIFLDDLKKVEKDIKQVAAYKVGELMSK
                     DIIKVHEDDKFDDVANVMIKKSINRVPVVDDDNKLKGIICRYDIIKALYNE"
     gene            complement(9554..9934)
                     /locus_tag="KRL67_002941"
     CDS             complement(9554..9934)
                     /locus_tag="KRL67_002941"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HBH1523807.1"
                     /translation="MNIFDIGVDIIEIDRIRKAVDKNNRFLEKIFTDREIEYFNSKNF
                     KVESIAGNFAAKEAISKSVGTGIRVFNFKDIEVLRNEMGKPIVKTYNNLAKMCIDYNV
                     LEIKVSISHSKDYAIANAITIIKD"
BASE COUNT         3211 a         1862 c         1059 g         3877 t
ORIGIN      
        1 aatgaacatt tatattataa cctattttta tattgataag tatgaaattg tttaaccaag
       61 tcaaaatgat atataaattt atatttaaat gtgtttagag tttaatttat tttcaatgtt
      121 taaaaaagtt attctatgca tctaatttaa ttagtaaact gaggttctag ttagataaat
      181 tatattacaa tgaatatata atatgcagtt tgcgatattt taaaagtaaa tgatataata
      241 tttggaacaa ctgggttgca atgtgttttt aaaaaatatc aaaataaggc tatgattttt
      301 aaaaccatag ccctgtctgt aaaaatttaa ttatcactta aaaaataaat ttttataaaa
      361 aaatacacat aaatatttcc agactccctc atgaactaaa aagttcattt tgtgacatct
      421 cttttataat gttctttatt ttatagtata agtttataaa aattaaatcc tatttatcaa
      481 tctgttctct aagccactta gttgctacat ctaactgttt attttcatct ttctcttcaa
      541 tagtaggctt tatacctttt tcattgatat aatctccatt aggtgtaaaa tattgagcag
      601 tagttagctt gtatcctgtt ccgtccttca atcttacaac tgattgtacc aaccctttac
      661 caaaagttgt agtacctaca gaaatgcctt ctttattatc aacgattgca gctgttaata
      721 tttcagcagc agaagcactt tctccattag ttaacactac tatcggaagt cccaattttt
      781 ctttatcaga tttcaaatac tcagtatttc ccttattatc ttttgtatat actatagttc
      841 cttcaccaat taactcatca gcaacttctt tacatatatc tagcattcca ccaggattat
      901 ttctaaggtc tattactaat cccttgatat tttttgtttt taattctttt aaagctttat
      961 caaagtcagt atatgtactt gatataaatt gggtaatttc tatatatcct aagtcattat
     1021 caattacttt actttctata ctcttctcaa ttatctggtc tgtttttact ttaaagttca
     1081 attgtttatc ttctcttaat atagtcaatt caactgtttt ccctttttta cccttcatca
     1141 tacgaacagc ttcatctgaa tttttatatg agacagattt tccattgact tttactaatt
     1201 tatctcctga ttttacacct gctttttctg ctggagagtc tttcatagga actactacta
     1261 agccaccatc ttttgatgcc cctatataca tacctacacc aacataagaa ccttcactat
     1321 cattaattag attctccatt tcatctttag tataataacc agaatactta tcatttgttc
     1381 ctagaaatag accttttgat gctccattta ccaaatcagt atctgatact tttttataaa
     1441 aatcttgttt tataatagat tctagtccta acaacttatc atatttttta tagtcttcat
     1501 agagttcttt cgatattaca actttattcc ccaatgtcaa ctgaaatgct gatgtaacca
     1561 tagctgttat aattactaaa atcaccccta aaaaaatagc tttcttcttt gaaatcacaa
     1621 gatacatctc cttttacata aactattatt attatatgac tagctgttat ttttattaca
     1681 tttacccaaa tatattgctt tattacaata aattatacta aaatttatat taaatacaat
     1741 tgctgtacac attataacat attaagggag gaattatttt atgaaaaaag aatttagaaa
     1801 aaaagttatt gaatcaagaa aaaataagag tgcaaacttc gtacttagta attcaaattt
     1861 aataactgaa aaattacttc aaatggaaga tataagaaat gcaactaata taatgcttta
     1921 tctaaatttt aacaatgaag ttcaaacaga taatttaatc aaaaaacttt tgtctctaaa
     1981 gaaaatagta tcaagtccca tcacaataaa agaaaatcat acattaatcc ctactcaaat
     2041 aacagattta aaagagggat tgagaattgg agcatatgga ataagagaac ctaacggaaa
     2101 atcacctaca attgatatta aggccttgga tgttgtaatt gtaccagctg tagcatatga
     2161 tatacattgc tatagattag gatatggtgg aggattttat gatagatttt tagaaaactt
     2221 aagaaaagat gctataacta taggcatagc atttgacttc cagatattcg attctattcc
     2281 aaaagaagct catgatgcac aattagacta catagttact gaaactagaa ttatcactcc
     2341 aaataattag aacattaagt acttccagac cttgtttaat atagtaataa attctactgt
     2401 taatatctta ctttataagc attttaagtc atggctttta caacaataag aaccatacac
     2461 aatttactta cattaataaa aaccatgact ttattgtaca atatgcagtt atttaattat
     2521 aaaatacctt cttatttcgt cgcatttaat tctattctat ctggaagttc actcatattt
     2581 gtttcaacaa ctatgtatgg aaaagaatct tccttatctt tatactttat cttgactctc
     2641 aaaacatcct ttccttcctc aaccacactt tctattttgt ccatttctat accatattta
     2701 ttatttcctc tagtcactat aatgtatatt ttgttatcga ttttagccgc taatgctctt
     2761 tcttcatttg tatacttatc catcacatct agtatctttt ctggtattga atttctttgt
     2821 aatactgtat aatctatagg tttagatttc tctacaaaca tacttggtat gaatataaat
     2881 ccccctataa cgaatacaag agctataact ccataaatta tacctttaat attaaacttt
     2941 acattcaatc ttatcactcc tcccatatat tctaaatata tgatttataa acttatctta
     3001 gactctaaat tattgattag tatatattta tccataatta taaatatatg taatgtaact
     3061 aaaaaacatg tttttcctat ttcataataa acgagctaaa aaataaaaag agctatatga
     3121 aaacattttc attgttttca tatagctctt gggtttacaa tattaaaaaa tatttatttg
     3181 tcactgattc cttcttgata tccagatgtt tttgtaatct gtctataaag aacacaaacc
     3241 agtattatga cgcccatata tccagtatat ggatataaaa taccaaccag cttatcaaaa
     3301 ggtaggaatc cacctataag acctaatata gctgctatta aagttattaa tttaaactta
     3361 ggatgattat catctacaaa tctattagtt actgaccata ataaaggtac agctgttgta
     3421 tatataccta atataagaat tattgagaat aaaactccaa ttattggtga aatcttatct
     3481 gctaagaata atgatggtat tgcttttgaa tataagtttc ctatatcaga aagaatagct
     3541 aagtgcatca ttatagccgc agccataagt gctactccac ctaatatacc accacaaata
     3601 gcttcttttt tgttagcagc acttgctcct aatccagtta agaatgccat aacaacaatt
     3661 gtgttataag tagtatatat tacaccagat agataagcat ttggaactgg tttagctatc
     3721 tttacagtgc taagaacttg tgcagcttct cctatattac ctatgttttt agctaaactt
     3781 ataccaccaa ctaaaagtgt aaagattatt attataggcc ctattccacc tagtatcttt
     3841 gataatctat caagacctaa tgaaacagtt aataatgcta ctactgccat acctactcta
     3901 cctacaaatg gatttaatcc atagtattca gttaaagttg ctcctgctcc tgaaatcatt
     3961 actacgaaaa caccaaataa gaataaaggt ccaaaccatt caaagaacat tcctaaatac
     4021 ttaccacaat aaacacgata aattttgata ggttctttaa gttgaagctc tttacctttt
     4081 gacataactg aagcacccat ccatgagaat aataccattg aaattatccc accaataata
     4141 cctgcaaaac cataaaaaga gaagaattgc attatttcct gtcctgttgc aaatccagaa
     4201 ccgattactg ttgctacata cgcacctgcg aaactgatga ctgttttaac atttactttt
     4261 tctgacatat ctcagttacc tccccatgtg aaattttttc ttctgcttaa acaattcgat
     4321 aaactaccta caatttcctt taacataaaa tacatgataa ctgttttata gctttttagt
     4381 ataataactt aaaacttaca atctaatttt ataccctagt cctcctttca taattttttt
     4441 agattctttt atctaagtag tcattatata taaaattaat ttctctataa attatatatt
     4501 agtcatattt ttctgaatag tcaatatata tttaaatatt ttctctattt ttgtaaaaat
     4561 ttgaatattt tattacattt ttgatatatt gatatcatat aaaaaaaaga gagatataaa
     4621 ttaatataac actttatatc tctcttttaa atatattata ttattaacaa acatgaataa
     4681 tttatatctc ttacattctt tttgcaataa tagcttcttt aacagtcttt actatgtcat
     4741 cacaagttaa ttcatatttt tttaacaatt catttggagt accagattct ccaaatgtat
     4801 ctttaattcc aactttcttt acaatggttg gacaagactc tcccactacc tcacttacag
     4861 cagaacctaa tcccccaatt atgctgtgtt cttctacagt tacaatagca ttcgtttctt
     4921 ttgctgattc taatattaaa tctttgtcta taggttttat cgaggacatg tttatgactc
     4981 tagcatttat accttcttct tgcaactttt cttgtgctaa tagagcttca tttaccataa
     5041 ttccacaagc tataattgat acatcctttc cttctcttaa gactgtacct tttcctattt
     5101 caaatttata atcttcatca aataaaactg gtactgaact tctccctaat ctaacataaa
     5161 caggtccatt atacttagct atttcaaaaa taattttttc tgtttcaact ccatcagctg
     5221 gaacaagaac tgtcatattt ggtatagctc tcattatagc aatatcttct acactttcat
     5281 gagatgctcc atcctctcca acagtaagac ctgcatgagt agcgcatatt tttacattta
     5341 gctttggata acaaactgaa tttcttataa tctcaaaggc ccttcccgta gcaaacatag
     5401 caaaagtact tgcaaaaggt attttaccag cagttgataa tccacaagca gcgcctatta
     5461 aattttgttc agctataccc atattaaaaa atctatctgg aaatgacttg taaaaatcat
     5521 gtgtttttgt agactttgat aaatctgcat ctaatactac aacattgtca tttatttgac
     5581 ctaattttac taatgcttta ccatatgctt ctctcgtcgc cattttactc attgtcagca
     5641 cctcctaatt caagcaaggc tttttctagc tcctcatcac ttggagctgt cccatgccat
     5701 cctgcttgat tttccataaa agatatccct ttgcctttta tagtctttgc aactatcatt
     5761 gtaggttttt taactgttga tttagctata tcaagagccg caaatatttg gtcaaaatca
     5821 tgtccatcta tctcaattac attccaacca aaagctttaa atttatcaac tattgaacct
     5881 aaactcatta cactttctat atctccatct atttgaagtc cattattatc taaaaaagca
     5941 attaaattat caagtttata atgtgcagca ctcatagcag cttcccaaac aattccttct
     6001 tgaacctcac catcaccaag caaaatatac acattccacg gagcattatc caactttgat
     6061 gccatagcca taccacatgc aactgaaaaa ccttgaccta aagaaccagt agatatttcc
     6121 acacctggcg tgcctttcat gtctggatga ccttgtaaca tccttcctat cttccttaat
     6181 ccattcagtt cttccttatc aaaatagcct ttttcagcca atgtagcata taatgctggt
     6241 gcagcatgtc cttttgataa tacaaatcta tctctatcct ccattttagg atttgaagaa
     6301 tccacattca tttcatcaaa gtataacgca gtaagtattt caactactga taatgaccct
     6361 cctggatgtc cagatttggc tctatgtatc attgaaacta tatctcttct tatgatacgc
     6421 gcaatctcat ttaatccttt atggtctctc atgcaattac ctcccttgag ttcgtaattt
     6481 tatatcaatt ccatattatt atatcacttt tatgctattt ttttgcgtta tttttaattt
     6541 tattaacata gtacattaaa attaaatcct aagagatatt taaaagaaaa aagggattgt
     6601 ctaataaaag acaaatccct ctttaaaaag taaacaatcc taaacttatt tgaagtccat
     6661 catctacttt tatcatcata ttttcatcta gacaaccaat tttttccctc agtctctttt
     6721 tatcaatagt tctaatttgc tctaaaagaa ttactgagtc tttattaagc ccatattcat
     6781 tggaacttat ctctacatga gtaggtaatt tggctttatt aatctgagat gttatagctg
     6841 atactataac tgtaggacta tatttattac ctacatcatt ttgtattatc agaacaggtc
     6901 taacgcctcc ctgctctgaa ccaacaactg gacttaaatc agcgtaatat aaatcaccac
     6961 gctttatttc acaattagta ctcaccttgg gcaatcttcc cttcattttt atcatcagaa
     7021 gatacttctt caactgtcat acctaattca gctagtgaca gattaatttg acccatttct
     7081 ctataacccg ttttcataga ttctttaata tgaattttct ttttctgagt tatatagaac
     7141 tgaaaagcag cttttgcaaa atcttctctg ttactgttct ccatctgaac tatactatca
     7201 acttcagaaa tcagagagtc aggtaaagta acttctattt tttctttact cttatcgtac
     7261 acaaaatcgc ctcccagatt ataatatcat ggttttattt tagcaaataa ctgtttattt
     7321 gtacaagctc attattttcc atataaacac ggtcaactct tcttgatatc atacataaca
     7381 cttcatagtt tatagttcct aagtctttag ctatacgctc agctgtaact tcgccttctc
     7441 caaatagtat aacctcatct ccaactttta tgtctatatc tttgtcaatt ctaaccatta
     7501 tttgatccat acatattcta ccaactacat caaacacttc tcccttaata agaacctttg
     7561 gatttttctg gattctagta aatccatctg cgtatcctat tggaactgta gctattgttt
     7621 ctttatctgt agttgtgtat tttagtccat aacttattcc tacacctggt tcaacttgtt
     7681 ttatatgtcc gatttttgat tttaatttca tggctggtct taattctaat ctatctttaa
     7741 atacatcatc agatggataa tgaccatata atattattcc tgctcttacc atatttaatc
     7801 ttaaatcagg gcaatccata atagctgcac tgtttgatac atgttttata gctattttta
     7861 cgccagcctc atctaattta tcagacataa atttataatt attagcttgt ttataagtgt
     7921 actctttact tacttcatca gctgtagcaa aatgagtaaa cataccttct aaatctatat
     7981 attctaattt atttagttct attatttcct gtactgactc ctcattaggt tggaaaccta
     8041 ttctagtcat ccctgaatct attttaacat gaacacaggc tttttctcct aaagattttg
     8101 caatttcatt tatcttttgt gctgtttcta aagaataaac tgtcatagtt attttatttt
     8161 ttatagaatc ttcaaaagct tcttctggag tatatcccaa gttcaaaata ggaagtgtta
     8221 tgccattttg tctaagttca attccttcag cagttcttgc tactgctaaa taatctactt
     8281 tttctttttc tagcaatttt gcaacttcta ctgcaccatg tccatatgca tcagctttta
     8341 ttactccaca aatcttaata tcttcttcta ataaattttt aatattattt aagttaaatc
     8401 ttaagttatc tagatttatc tctgcccatg taggcactgt tattttttgc atgtctttag
     8461 tcccccttag tttctgtatt caaaatcttt gtattttact gtaaatcttg gcactccctc
     8521 tttatctaac acttccattt tttcaggatt ttttgtgtca gcatttacat ataatacttg
     8581 cttgttaaaa tatttattat ccccaggaat aaaagtctct aaaaccaaat gtcctttgga
     8641 taactttact ttcatctcct cattttgaag atagtttttt ataaagtctc ctacaaaaag
     8701 atattgatta ttttttcctg tattaggtaa ctccactaca tcactaatct ttggattttt
     8761 aactaatatt ttatcacctt gatattctat acttttccct tttaaatgtt ttggggaaat
     8821 tacttctaat ttatagttat cggttttttt gtaagtatga attaaaacat aattgtgtgg
     8881 gcttttattt ccaactactt ctacttcagc tttacaactg taataattca tatcagaaat
     8941 ctgcttttga aaatctttgt atacttcttc ttttgtggac tgtcgttttt ggcatcctat
     9001 tacaattata accagtgcca agaacataat acatactatg gtccactttt ttctcacatc
     9061 acacctccta cttcagttta ttcattgtat aaagctttta ttatatcata tctacaaatt
     9121 atacctttaa gtttgttgtc atcatctact acaggaactc tgtttattga ttttttaatc
     9181 attacattag ctacatcatc aaacttatca tcttcatgaa cctttattat atccttagac
     9241 ataagttctc caaccttata agcagctact tgttttatat ctttttcaac tttttttaag
     9301 tcatctaaga aaatcaatcc ttgtaataga tttatataga gaggtgcttc tatatatttt
     9361 tcttttttaa gaatatctgt ttcagaaatt attccaacaa ctctattctc tgaatctaca
     9421 actggcagac ctcctatttt ttctgcaatt agcatattag ctacatctgc tatactatca
     9481 tcttgttttg ctactattac atctgttgtc attatttctt ttgctgtttt atccatcata
     9541 atgtaatcac tccttagtcc ttaattatgg ttatcgcatt tgctatagca taatcttttg
     9601 aatgtgatat tgaaactttt atttctaaaa cattataatc tatgcacatt tttgctagat
     9661 tattataagt ttttactatt ggttttccca tttcatttct gagaacttct atatctttaa
     9721 agttaaatac tctaattcct gtacctacag atttacttat agcttcctta gctgcgaaat
     9781 tacctgctat actttctact ttaaaattct tagagttaaa atactctatt tctctatctg
     9841 taaatatctt ctccaaaaac ctattatttt tgtctacagc ctttcttatt ctatcaattt
     9901 caattatatc tacgccaata tcaaatatat tcattgttcc tccagaaaat ttataatact
     9961 tctttcaata attaataatg aatttataaa tttactatcg ataatacat
//
LOCUS       DAEPXF010000145         9962 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain HB27
            SAMN13376389-rid12479423.denovo.152, whole genome shotgun sequence.
ACCESSION   DAEPXF010000145 DAEPXF010000000
VERSION     DAEPXF010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13376389
            Sequence Read Archive: SRR10518432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 9962)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9962)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001069624.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001069624.1
            Genome Coverage       :: 315x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/15/2021 01:54:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,165
            CDSs (total)                      :: 4,076
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 89
            rRNAs                             :: 10, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 10 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 19 of 69
            Pseudo Genes (incomplete)         :: 44 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 10 of 69
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9962
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13376389-rid12479423.denovo.152"
                     /strain="HB27"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="China: Hebei"
                     /collection_date="2017"
     gene            complement(121..864)
                     /locus_tag="KRL67_002942"
     CDS             complement(121..864)
                     /locus_tag="KRL67_002942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase"
                     /protein_id="HBH1523808.1"
                     /translation="MNFLSDLHTHSIVSGHGYSTLLENINYCKENGIKILGTSEHGPK
                     MPGSPHRWYFHNIQNIPRIINNIIILRGCEANILDIKGNIDLEPFVIPRLDYLILSFH
                     EAVFSPNTLENNTKALINSINKHDNIEILGHLGNPNYPIDYELIIKLAIEKNILIEIN
                     NCSIKGVSRNGSSDNCKYIATLCKKYGAKIILTSDAHICFDIGNYEYSENILKEINFP
                     DELIMNYPKKLINHFHQKGKLLDVDYTNI"
     gene            1030..3597
                     /locus_tag="KRL67_002943"
     CDS             1030..3597
                     /locus_tag="KRL67_002943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBH1523809.1"
                     /translation="MTQKKLSRKEKVLICLKRLTEEHLNFNNGIRFGFDAEYIATEVG
                     INRSNASKELNQLIKESIIIKIKGKPVQYLYKESIESILGKKLNKGIFDNINSINEYS
                     ENDSIKDEYILESIKKENNIFNLVGCDGSLKSQIEQAKAAVIYPPKGLSTLIIGPTGV
                     GKSIFAEYMYKYSLTIKEDSDNAPFIIFNCADYSDNQQLLLAQLFGYVKGAFTGADKD
                     KYGIVHEANNGILFLDEVHRLSAEGQEMLFLLMDKGIYRRLGEANKVHECRVMIIAAT
                     TEDPETAMLETFLRRIPVTIKIPSLEERGFQERMKLICYFFKEESIRIGAKLKVSKEV
                     IKAFILYKCKGNIGQLKSDIQLICARAFLDYMTYKRECVYVRLSLLPNNIRESLYGNN
                     RKEEFVQNFSFIGKDDIIFLPEEKNFESENLLIENKKYDLDFYGMIKETWNKLQQEGI
                     KESQIRSVIDEDIQKYSYNLMNTFIYNSSNQTAYNNIVNDSIATTVKYILQKHDKWSK
                     REGLDKLIKAIALHIQNLIERIKIGNIVKHPNKDEIMKERAYEYNIAKEILSNMSLMY
                     GVEFPEDEAIFLATFLYLSYVGLNEESIAILVIMHGESTASSMVNVANTLLDCNHAVA
                     INMGLEDRVQDILVQAVEIANKIDKGKGILILTDMGSILTFAKVIRDATGKEVKAIDM
                     VSTPIVIEATRKALTPEMTLEKLYFNIIESIKKHYGDMEVAYAEKDNGGRYFDRLLID
                     NISKTLTFLNGEKAYFILKEVINRISEHYSLVIQDELLVKFIFHCSCMIERVIIKESL
                     VYKSYEERISRNKELYLVIKESFKLVEETFDIIISDMEYANILDIFESQYDTDKFKS"
     gene            3827..4231
                     /locus_tag="KRL67_002944"
     CDS             3827..4231
                     /locus_tag="KRL67_002944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBH1523810.1"
                     /translation="MIGIIVMTHGSFSEEIIKSCELIAGPAERTAAIKLNRNDNIEDL
                     NKNFVEKLNELDEGDGVLVLADLLGGSPSNVASLNLKKGGKFHALTGVNLPMLLEALI
                     NREGKSLEELAEACIEAGQTGINNINKVLSSM"
     gene            4255..4752
                     /locus_tag="KRL67_002945"
     CDS             4255..4752
                     /locus_tag="KRL67_002945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBH1523811.1"
                     /translation="MSLVKYCRCDDRLIHGQVIYKWVKHLGVKKIVVVDDETTNDVIA
                     KGLIKMAAPKNIDLSILTVSESRRYFYNNQADDNVFVLIKNLDTANRMIEEGINIKKL
                     IVGRIPTGIGKKKISQNVYINKKEFLLIDEFIKKNINVSIQMVPDEEEVELNHNINRY
                     KGEFI"
     gene            4755..5156
                     /locus_tag="KRL67_002946"
     CDS             4755..5156
                     /locus_tag="KRL67_002946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIC"
                     /protein_id="HBH1523812.1"
                     /translation="MNKYKVIILCHGTWGKVLVEETNKNFGLSNQYEVLSLSSEKDVS
                     VFMKEVEEVVDKEDVKIIISDLYGGSTSSVAIAVAIRRGIDAINGLSLQTILIVDEEL
                     SKEDKLHTLPERIVKKNNNLCVDLIKEFKKI"
     gene            5174..5662
                     /locus_tag="KRL67_002947"
     CDS             5174..5662
                     /locus_tag="KRL67_002947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBH1523813.1"
                     /translation="MAKITLVRVDHRLIHGQVITKWVKIAQAQKIIIVDDFLGQDEFM
                     ADIYKMAAPSGVEVVILTAEDAGQAFQNNTLGDKNIFILFKNVDMANKAYKAGLKYEK
                     IQLGGIPNEAGKKMVFTAVSLGNEDVEQLNELNENGVEIVLQVIPEESSMTYENALKK
                     FK"
     gene            5689..6444
                     /locus_tag="KRL67_002948"
     CDS             5689..6444
                     /locus_tag="KRL67_002948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBH1523814.1"
                     /translation="MESVLMLAIVTGLWYWFAAGLAGYTLFSTLKSPLFIGFSLGLLW
                     GDVTTGMIVGASIEMVYLGMVAAGGNIPSDKCLAALIAIPVALQTGVNAEVAVSIAVP
                     LGVIGVLVNNLRRTGNAVLVHKADKYAEEGNTKGIWRCATLYSLIFGFVLRFPIVFVC
                     NFFGADLVQSLLDVIPQWLMNGLTVMGGILPALGFATTIFTIGKNKFLPMFIIGFFMV
                     QYFEISITAAAIFGVCIALLITFMKEDKRVGEV"
     gene            6444..7307
                     /locus_tag="KRL67_002949"
     CDS             6444..7307
                     /locus_tag="KRL67_002949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HBH1523815.1"
                     /translation="MQEIKNKEVKNGQSPTEVNKTRVLTKKDVTKTYLRWWWTAELSN
                     SFERMQALAVCASFTPALEKLYKRKEDLVDALKRHLQFFNTQAIWGGLIHGTVLAMEE
                     EKATEGKIPGEVISGVKNGLMGPLAGIGDTLDFGTFQTIFLALGASFGAEGSVIGAFF
                     PIMFSILLFCEGYYLFHLGYSLGRDSIKKILSGGIVNKIIDGASILGMFMMGALSATT
                     VKLSTPLSFDIGGKAIVVQDTLNMIAPGLLPLGVVFFVYWGMKYKKWTITKLLVILVV
                     LALVGSFIGIF"
     gene            7354..7833
                     /locus_tag="KRL67_002950"
     CDS             7354..7833
                     /locus_tag="KRL67_002950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-2-amino-thiazoline-4-carboxylic acid
                     hydrolase"
                     /protein_id="HBH1523816.1"
                     /translation="MSEKMYTEKEYLEGIRGATGERAVWFYLLMKEAEKLGVNPDNIC
                     KEAIYGFGKMRGQKYNVADTPGKMAEMLYNSKGQKVFEMELVENTDENGVLKFHHCPL
                     DAAWKEYGLTKEERKEICRLACYGDYGRVDCAQGIKLEFAQKCAHDDEVCELVFTRK"
     gene            7898..9205
                     /locus_tag="KRL67_002951"
     CDS             7898..9205
                     /locus_tag="KRL67_002951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HBH1523817.1"
                     /translation="MDINRSVDNSLNRTIEFLKELIKIDSQQGEPISQCPFGIGPKKS
                     LDKTLDYCASLGFSVKNIDNYIGYAEIGEGEELIGIPMHLDIVPPGEGWSVDPFSGAV
                     IDNIIYGRGVIDNKGAVSMLIHVLKNIEDMYPTINKRIRLIFGTNEETGMECIKYYLD
                     KGEEIPSMGFTPDAMYPVVNGEKGRVHIRIEKEIKIDKSKPYIIVGGGTKENVVPSHC
                     TAKIINGIISELTTKGVAVHASNPEKGENAISKMVIKIVEDNMDFQHREDIELVSKYL
                     CSDYYGDALGINQYDEVFKNTTLNLGILKVNEEKIVCELDIRYGKNIALNNIIDRFKK
                     VFCNGWKIGVIAHKDLHYVDESNLVLKKLLEAYEEVTDENGYTIAMGGGTYASWFKDM
                     VAFGPKFLAYKTGGHGVDERVPINHIRKNMEIYTLALIKLLEL"
     gene            9516..9656
                     /locus_tag="KRL67_002952"
     CDS             9516..9656
                     /locus_tag="KRL67_002952"
                     /inference="COORDINATES: protein motif:HMM:NF021468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2185 domain-containing protein"
                     /protein_id="HBH1523818.1"
                     /translation="MNGEGCILSNKITKECYKIGYMIREKSIDTYPDSGWGFMTWDEN
                     GE"
BASE COUNT         3924 a          975 c         1892 g         3171 t
ORIGIN      
        1 tcaagttcat aaaagtaatt aagctttatt aaatagcttc tttattataa tttctttatt
       61 tcatataata taaatagtat taaaattttt atgagagtta aagtaagcat gtattattac
      121 ttaaatattt gtataatcaa catctagtaa tttacctttt tggtgaaaat gattaataag
      181 ttttttagga taattcatta ttaattcatc tggaaaatta atttccttta agatattctc
      241 agaatactca tagttaccta tgtcaaaaca aatatgagca tctgaagtta gtatgatttt
      301 agcaccatat tttttgcaaa gtgtagcaat atacttacaa ttatccgagc taccatttct
      361 tgacacgcct ttaatagaac agttatttat ttctattaag atattctttt ctatagctaa
      421 tttaatgatt aattcatagt cgattgggta gtttgggttt ccaaggtgtc ctaatatttc
      481 aatattatca tgcttattta ttgaatttat taaagcttta gtattattct ctaatgtatt
      541 tggagaaaaa acagcttcgt gaaatgacaa gataagataa tctagccgag gaattacaaa
      601 gggctctagg tctatgtttc ctttaatatc tagtatattt gcttcacagc ctcttaaaat
      661 tataatgtta tttatgattc ttgggatatt ttgaatgttg tgaaagtacc atctgtgggg
      721 agaaccaggc atcttaggac catgttcaga tgtacctagt atcttaattc cattttcttt
      781 acaataatta atattttcta gtaaagtgct atagccatga ccactgacta tagagtgggt
      841 gtgtaaatct gataaaaaat tcatataaat tcctcctcta taaaagatga gggtaacgta
      901 atttatatta taaaacgaat aggaaagaaa atcaatagtt acataattag acaaaaatat
      961 taaaatattg tagaattaag cttaataata aatgtttaac agtattactg cttatagggg
     1021 aggttaacta tgacacaaaa gaagctatca aggaaagaaa aagttttaat ttgcttgaaa
     1081 aggttaacag aagaacactt gaactttaat aatggcatta gatttggatt tgatgcagaa
     1141 tatatagcta ctgaagtagg tataaataga agtaatgcta gtaaggaatt aaatcaatta
     1201 ataaaagaaa gtattattat aaagataaag ggtaagcctg ttcagtactt atataaagaa
     1261 agtattgaga gtattttagg aaagaaacta aataaaggga tatttgataa tattaactct
     1321 ataaacgaat atagtgaaaa tgattctatc aaagatgagt atattttaga gagtataaaa
     1381 aaagagaaca atatttttaa tttagtagga tgtgatggaa gtttaaaatc ccaaatagaa
     1441 caggctaaag cagcagtaat atatccacct aaggggttga gcacattaat aataggacct
     1501 acaggagttg gtaaatctat atttgcagaa tatatgtata agtatagttt aactataaaa
     1561 gaagatagtg ataatgcacc atttatcata tttaactgtg ctgattattc tgataatcag
     1621 caattattat tagcacagct ttttggatat gtaaaaggag catttactgg ggctgataaa
     1681 gataagtatg gtattgtaca tgaagctaat aatggaatat tgtttttaga tgaagttcat
     1741 aggttatctg cagagggaca agaaatgcta tttctgctta tggataaagg catatataga
     1801 aggttaggag aggctaataa ggtacacgag tgtagggtta tgataattgc tgccactaca
     1861 gaagacccag aaacagcaat gttggaaacg tttttaagaa gaatacctgt aactataaag
     1921 attccgtctt tagaagaaag aggttttcaa gagagaatga agcttatatg ttattttttt
     1981 aaagaagaga gtattaggat tggtgctaag ttaaaggttt caaaagaagt cattaaggca
     2041 tttattttat ataagtgtaa gggaaacata ggacaattaa aaagcgatat acaattgatt
     2101 tgtgcaagag cttttttaga ttatatgact tataaaaggg aatgtgtata tgtaaggctc
     2161 tctcttctac caaataatat tagagaatca ttatatggaa ataatagaaa agaggagttt
     2221 gtacaaaatt tcagttttat tggaaaagat gatattattt ttttaccaga agaaaagaat
     2281 tttgaaagtg aaaatttatt aatagaaaat aaaaaatatg atttagattt ttatggaatg
     2341 ataaaagaaa cttggaataa acttcaacaa gaaggtatta aagagtcaca aattagaagt
     2401 gtcatagatg aagacataca gaagtattcc tataatttaa tgaatacttt tatatataat
     2461 agtagtaatc aaacagctta taacaacatt gttaatgatt caattgcaac tactgtgaag
     2521 tatattttac aaaagcacga taaatggagc aaaagagaag gattagataa attaataaaa
     2581 gctatagcct tacatattca gaatttaatt gaaagaataa agattggaaa catagtaaag
     2641 catcctaata aagatgaaat tatgaaggaa agagcatatg aatataacat agcaaaagaa
     2701 atattatcta acatgtcatt aatgtatgga gttgagtttc cagaagatga agcgattttt
     2761 ttagcaacat ttctttattt atcgtatgtg ggattaaatg aggagagtat agcaatatta
     2821 gtcataatgc atggtgaatc tacagcatct agcatggtga atgtagccaa tactttactg
     2881 gattgtaacc atgcagtggc aattaatatg ggattagaag atagagttca ggatatttta
     2941 gtacaagctg tagaaatagc taataaaatt gataaaggaa agggaatttt aattttaact
     3001 gatatggggt ctattttaac atttgctaaa gttattcgtg atgctacagg aaaagaagta
     3061 aaagcaatag atatggtaag tacaccaatt gtaatagaag caactagaaa ggccctaact
     3121 ccagaaatga cattagagaa attatatttt aatattatag aatctataaa gaaacattat
     3181 ggagatatgg aagttgcata tgctgaaaaa gataatggtg gtagatattt tgatagatta
     3241 ctaattgata atattagtaa aactttgact tttttaaatg gggaaaaggc ttattttata
     3301 ttaaaagagg taattaatag aattagtgaa cactattctt tagtaattca agatgaactt
     3361 ttagtaaagt ttatatttca ttgttcatgt atgattgaaa gagtaataat aaaagagtct
     3421 ttagtttata aatcatatga agaaagaatt agtagaaata aagaattata tttagttata
     3481 aaagaaagtt ttaagctagt agaagagaca tttgatataa ttatttcaga tatggaatat
     3541 gcaaatatat tagatatatt tgaatcacaa tatgatacag ataaattcaa aagttgatac
     3601 aaaatttaaa aatagccaaa aggctatctt ttttttactt tttttgatac agatttaata
     3661 caggttgaaa aaaccttttg atacatattg aaatagtaag ttttttaaaa aaaataagta
     3721 aaaaggtttg attaaatcct gtaaaatagc catttattat tgatacacat aagttggcac
     3781 ggcaattgct tatataaaaa gtgacaagaa aagtgaggtg aaattggtga taggaataat
     3841 agttatgact catgggagtt ttagtgaaga aattataaaa agctgtgaac ttattgcagg
     3901 accagcagaa agaacagcag caattaaact aaataggaat gataatattg aagacttaaa
     3961 taaaaatttt gtagaaaagt taaatgaatt agatgaaggt gacggagttt tagtattagc
     4021 tgacctatta ggaggttcgc caagtaatgt tgcaagttta aaccttaaaa aaggtggaaa
     4081 gtttcatgca cttactggag taaatcttcc aatgctactt gaagctttaa ttaatagaga
     4141 gggaaaaagt ttagaagagt tagcagaagc atgtattgaa gcaggacaaa ctggtataaa
     4201 caatattaat aaagttctta gctcaatgta atagaaaaag gagtttgatt aaaaatgtct
     4261 ttagtaaaat attgcagatg tgatgataga ttaatacatg gtcaagtgat atataaatgg
     4321 gttaagcatc taggtgtaaa aaagatagta gtagtagatg atgaaacaac caatgatgtt
     4381 attgctaaag gtttgataaa aatggcagca ccaaagaata ttgatttatc aatattaact
     4441 gtaagtgaat caaggagata tttttataat aatcaagctg atgataacgt ttttgtatta
     4501 attaaaaatt tagatactgc aaatagaatg atagaagaag ggattaatat caaaaaactt
     4561 attgttggaa gaatacctac aggaatagga aaaaagaaaa tatctcaaaa tgtatacatt
     4621 aataaaaaag agtttctttt aatagatgaa tttattaaaa aaaatattaa tgtatcaatt
     4681 caaatggtac ctgatgaaga agaagtagaa ttaaaccaca atattaatcg ctataaagga
     4741 gagtttattt agcaatgaat aaatataagg taatcatact gtgccatgga acttggggaa
     4801 aagtattagt agaagaaaca aataaaaact ttggattgtc aaatcaatat gaagttttat
     4861 cactaagttc tgaaaaagat gtctctgtat ttatgaaaga ggttgaagaa gtagtagata
     4921 aagaagatgt gaaaattata atatcagatt tatatggagg atctacatca agtgttgcaa
     4981 tagctgttgc tataagaaga ggaatagatg caataaatgg acttagctta cagacaattt
     5041 taatcgtaga tgaagaatta agcaaagaag ataaattaca tacattacca gaaagaatcg
     5101 tcaaaaaaaa taataacctt tgtgtggatt taattaaaga atttaaaaaa atataaatta
     5161 tgaggtgaat ataatggcaa aaataacatt agtaagagta gaccacagat taattcatgg
     5221 acaagttata acaaaatggg taaaaatcgc tcaagctcaa aaaataataa tagttgatga
     5281 cttcttaggt caggatgaat ttatggctga catatataaa atggcagcac catcaggagt
     5341 agaagtagtt attttaacag cagaagatgc tggacaagca ttccaaaaca acactttagg
     5401 agataaaaat atatttattc tatttaaaaa tgttgatatg gcaaataagg cttataaagc
     5461 tggacttaaa tatgaaaaga ttcaattagg aggtattcca aatgaagcag gaaagaaaat
     5521 ggtgtttaca gctgtttcat taggaaatga ggatgtagaa caacttaatg aacttaatga
     5581 aaatggggtt gaaatagttt tacaagttat accagaggaa tcatctatga cgtatgaaaa
     5641 tgcgttaaag aaatttaaat aaaaaatata aattaggagg aattataaat ggaatctgtt
     5701 ttgatgttgg caattgtaac aggattatgg tattggtttg cagcaggtct tgcagggtat
     5761 acccttttct caacactaaa gtctccttta tttataggtt ttagccttgg attactttgg
     5821 ggtgatgtaa caactggtat gattgttggt gcaagtatag aaatggttta tctaggaatg
     5881 gttgcagcag ggggaaatat tccatcagat aaatgtctag ctgctcttat tgcaatacca
     5941 gtagcacttc aaactggtgt taatgcagaa gttgcagtat ctattgcggt acctttaggt
     6001 gttataggag tattagttaa taacttaaga agaactggca atgctgtatt agttcataag
     6061 gcagataagt atgctgaaga aggtaataca aaaggcatct ggagatgtgc aacactttat
     6121 tctttaatat ttgggtttgt tttaagattt ccaatagttt ttgtatgtaa cttttttgga
     6181 gcagatttag ttcaatcatt attagatgtt ataccacaat ggttaatgaa tggattaaca
     6241 gtaatgggtg gtatattacc tgcattaggt tttgcaacaa caatatttac tataggtaag
     6301 aataaattct taccaatgtt tattatagga ttttttatgg ttcaatattt tgaaatatca
     6361 attactgcag ctgcaatatt tggtgtttgt atagctcttt taattacctt tatgaaagaa
     6421 gacaaaaggg taggtgaagt ataatgcaag aaataaaaaa taaagaagtc aaaaatggac
     6481 aaagtccaac tgaagttaac aagacaagag tacttacaaa gaaagatgta acaaaaacat
     6541 acctaagatg gtggtggaca gcagaattat ctaactcttt tgaaagaatg caagcattag
     6601 cagtttgtgc aagtttcact ccagctttag aaaagttata taagagaaaa gaagatttag
     6661 tagatgcgtt aaagagacac ttacaatttt ttaatactca agctatatgg ggaggattaa
     6721 ttcacggtac agttttagct atggaagaag agaaagctac tgaaggaaaa atcccaggag
     6781 aagttatttc aggtgttaag aatggtctta tgggaccttt ggctggaatt ggagatactt
     6841 tagattttgg tacatttcaa acaatattct tagctttagg tgcttcattt ggagcagaag
     6901 gaagtgttat aggtgcattt ttcccaataa tgtttagtat attactattt tgcgaaggtt
     6961 attacttatt ccatttagga tattctttag gtagagattc aattaagaaa attctatcag
     7021 gtggtattgt caataagata attgatggtg cttcaattct aggaatgttt atgatgggag
     7081 cattatctgc aactacagtt aagctttcaa ctccgctatc atttgatatt ggtggtaaag
     7141 caattgttgt tcaagataca ttaaatatga ttgctccagg tttattacca cttggtgttg
     7201 tattcttcgt atattgggga atgaaataca agaagtggac aataactaag ttattagtaa
     7261 ttttagttgt attagcattg gtaggatcat tcattggaat attctaattt acagtgatta
     7321 aaatatagaa tttaatttta ggaggagaaa gaaatgtcag aaaaaatgta tacagaaaag
     7381 gaatatttag aaggaattag aggagcaacg ggagaaagag cagtatggtt ctatcttctt
     7441 atgaaagaag ctgaaaaatt aggagttaat ccagataata tatgtaaaga agcaatatat
     7501 ggatttggta aaatgagagg tcaaaaatat aacgtagcag acactccagg aaagatggca
     7561 gaaatgttat ataacagtaa aggacaaaaa gtgtttgaaa tggaattagt agaaaataca
     7621 gatgagaatg gagttttaaa attccatcac tgtccattag atgccgcttg gaaagaatat
     7681 ggattaacta aagaagaaag aaaagaaatt tgtagattag cttgttacgg tgattatgga
     7741 agagttgact gtgcacaagg cattaaatta gagtttgctc aaaaatgtgc tcatgatgat
     7801 gaagtttgtg aattagtgtt tacaagaaaa taatttttaa tgctaaaagc ctgttcttta
     7861 aggacaggct ttaaaaagat agaaagcagg ttgatttatg gatataaaca gaagtgtaga
     7921 taatagttta aatagaacca tagaatttct aaaagaattg ataaaaattg attcacaaca
     7981 aggtgaacct atttcacaat gtccttttgg tataggacct aaaaaatctt tagataaaac
     8041 tttagattac tgtgctagtt taggttttag tgttaaaaat attgataatt atattggata
     8101 tgcagaaatt ggagagggag aggaattaat agggattcca atgcatttag atatagttcc
     8161 accaggagag gggtggagtg tagacccatt ttcgggagca gtaattgaca atattattta
     8221 tggaagaggt gtcatagata ataagggggc tgtatctatg cttatacatg tattgaaaaa
     8281 catagaggat atgtatccta caattaataa aagaataaga ttaatttttg gtacaaatga
     8341 ggaaacaggt atggaatgta tcaaatatta cttagataaa ggtgaagaaa ttccaagtat
     8401 gggatttact ccagatgcta tgtatccagt ggttaatgga gaaaaaggaa gggttcatat
     8461 aaggatagaa aaagaaatta aaattgacaa aagtaaacct tacataatag tagggggagg
     8521 aactaaagaa aatgttgttc cgtctcattg tactgcaaaa attattaatg gaattatttc
     8581 agagttaact actaaaggag tagctgtaca tgcaagtaat cctgaaaagg gagaaaatgc
     8641 tatatctaaa atggtaataa agatagttga agataatatg gattttcaac atagagaaga
     8701 tatagaatta gtttcaaaat acctttgtag tgattattat ggagatgcat tggggattaa
     8761 tcaatatgat gaggtattca aaaacactac cttaaattta ggaatattaa aagtaaatga
     8821 ggaaaaaatc gtatgtgagt tagatataag gtatggtaaa aatatagctt taaacaatat
     8881 aatagataga ttcaaaaagg ttttttgtaa tggttggaaa ataggggtta ttgctcataa
     8941 agatttacat tatgtggatg agagtaactt agtacttaaa aaattattag aagcctatga
     9001 agaagtaaca gatgaaaatg gatatacaat tgctatgggt ggtggcacat atgcaagttg
     9061 gtttaaagat atggtggcgt ttggacctaa gttcttagcg tataaaactg gtggtcatgg
     9121 agtagatgaa agagtaccaa taaatcatat acgaaagaat atggagatat ataccttggc
     9181 tttaataaaa ctattagaat tgtaaaagga gaagagactt tatgtgcaaa tgtacggata
     9241 tcttaaaaag agagataaaa cgagttcaac tttatttaaa tgttcaacac catcaaatgg
     9301 ggctgtctca aaatagagat aagcctctta cttattgttg aaatgatgaa aatatatttt
     9361 acttcataca aaaaagagtg ccttatgaag taaaaagtta attttgagac accctcattt
     9421 gatgatgttg aagagaaaat aagtatgaaa ttattaaaaa aagatgattt tataaaagtt
     9481 aaggttgaaa agaaaagtta attgaatgga attagataaa tggagaaggt tgtatactat
     9541 ctaataaaat aacaaaagag tgttataaaa ttggttatat gattagagaa aagtctattg
     9601 atacttatcc agatagtgga tggggattta tgacttggga tgaaaatgga gaataataaa
     9661 aattatagtt tatacaattt aataagatat atgtacaagc agttaatcta aacgattgac
     9721 tgcttttctt ttatagattt ttatataata gacaattacc aatacatgat agttgcctgt
     9781 tataattaga ggaataaata cgaataataa ctttataaaa gaattaaatg gaaaattgaa
     9841 gagttatatt caggatttta aaaagaatat gaaatgagaa atgaaaataa ggtgtttata
     9901 ttaaaagagg ttttacacaa aatatttaaa aacgtgataa ataaaaactg tatttagata
     9961 ga
//
LOCUS       DAEPXF010000146         9949 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain HB27
            SAMN13376389-rid12479423.denovo.153, whole genome shotgun sequence.
ACCESSION   DAEPXF010000146 DAEPXF010000000
VERSION     DAEPXF010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13376389
            Sequence Read Archive: SRR10518432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 9949)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9949)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001069624.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001069624.1
            Genome Coverage       :: 315x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/15/2021 01:54:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,165
            CDSs (total)                      :: 4,076
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 89
            rRNAs                             :: 10, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 10 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 19 of 69
            Pseudo Genes (incomplete)         :: 44 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 10 of 69
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9949
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13376389-rid12479423.denovo.153"
                     /strain="HB27"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="China: Hebei"
                     /collection_date="2017"
     gene            complement(104..1426)
                     /locus_tag="KRL67_002953"
     CDS             complement(104..1426)
                     /locus_tag="KRL67_002953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent Fis family transcriptional
                     regulator"
                     /protein_id="HBH1523819.1"
                     /translation="MKILVVDDELEYGVVMKKILQKKGHLVDIALSGEEAINIIKKDK
                     NYDLILSDVMMKNMDGVQLLDRIKSINRDIEVILVTGYGSIENAVDAMKRGALSYFIK
                     SNPIENLLEEVEKVKASKISVSVQKNNLEFTLESKNRDFNNIIKIAKKAACKDVNILI
                     LGESGVGKDILARYIHSISPRKNEIFVPVNCCSFSENLLESELFGHEKGSFTGAVDSR
                     KGRFELSNKGTLFLDEIGDIPLNVQVKLLRTLEDKSIERIGSNKSIKVDFRLICAMNK
                     EPKVEISNGNIREDFFYRISTITITIPPLRKRREDLTTLIEFFLNKYQFEHDKKIHSI
                     DKEVKDFLLNYNYPGNIRELKNIINRLVVLSEEGNLSKDNLNLISNNVYIDDKISIRP
                     LREIRKEFECEYIEKVLSLCGNNISNTAKKLEISRRQLTNKISEYNIK"
     gene            complement(1426..3420)
                     /locus_tag="KRL67_002954"
     CDS             complement(1426..3420)
                     /locus_tag="KRL67_002954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HBH1523820.1"
                     /translation="MNKKKIVIIGIIYSFLVVFSLTNMYVNMEYNLNVFEYIKKSLPF
                     TEEEKKWLEKHKNLIYSSDQSSPPLRYKGKEDGQYKGIIVDLINSLSIQIGRDFYFKP
                     NNWWKESFVNSIDDSIKFFDLIPSKERANKFIFTDPIYTLSANILKDKKSQDINSYMD
                     LKGKTVAIPEGDYSINFLKQKIQDINILLTPDIKTGVNHLMSGKVDAVVGDEPVLRYY
                     INNYGLSNKYSVLSNPIYTKKAVLAVPKQYEELVSILNKGIFKLQKNGVYKDLKKKWY
                     STYNEVDDILYERGIVPSIYLFIGIILISIYVFYSYTYLLKIEIKRKTEQVIENKKTL
                     EATFNSITDIIMLVDENNNIVESNKVLYDFMGEMSYKIADLINMIKGVIENTFSENTN
                     KTSEIEIHNKILKINTFPVEYKKNNTEYIVVLIKDITNDKIVEAKLLRENKMISIGQL
                     ASGVAHEIRNPLGIIRNNCYLLKDNVTMEEVNDCVKSIESNVDRASNIITNLLNFARI
                     SDDNLEHINIRNFIENIVKLQYKMLQLKNVEIKIDCEHNLVCYINGESLKHVFINLIS
                     NSIDAIYQDGKIIIYCYEKNHCLFIDFKDNGEGIKEDALKDIFNPFYTTKPIGEGTGL
                     GLYITYNEIKKNNGDISVESKLGVGTCFHIKIPLNKEVTI"
     gene            complement(3507..4754)
                     /locus_tag="KRL67_002955"
     CDS             complement(3507..4754)
                     /locus_tag="KRL67_002955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntP family permease"
                     /protein_id="HBH1523821.1"
                     /translation="MQITTFGAIFGLLIAIILIIKKFQAVYSLMLGAFIGGLVGGANI
                     TQTVDFMANGAMNISPSILRALASGVLAGSLIKTGAVDKISEQIVKIFGEKRALLSIV
                     ISTMVLAGVGVNLDVSIITVAPIGLYIGRKLNYSKLSILLAMLGGGKAGNIISPNPNT
                     IAVADNFSVNLSSVMMANIIPAIIGVVITVILASILINKGNKVQSYEILEQREDLPSL
                     FKSLCGPIIAIFLLFLGNVSPIVIDPMIALPIGGIVTLIVTGNLNNSREYLAFGLSKM
                     QGVCILLLGTGTIAGIIQMSELQQSTIGALQFLNMPQFLLAPVSGILMSLATASSTAG
                     ATIASSTFHDAIINGGLSPISGASIVNAGSSVFEQLPHGSLFHTSAGSINMGIGERFK
                     LIPYEALIGIVMTIISTSIQLVL"
     gene            4982..5527
                     /locus_tag="KRL67_002956"
     CDS             4982..5527
                     /locus_tag="KRL67_002956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBH1523822.1"
                     /translation="MNDLNLGEKIAEVRKKQNLSIRDLAKLADVTPSLLSQIERGLAN
                     PSVNSLKSIASSLNVPLFTFFVSEVDKKNLIVRHNNRKKVILPGSKEVIYEILIPDSS
                     GNLEFAIMDLAPNTSSCVDRITHNGDEIAYVLEGEVKLFMDDDEFTLSKGDSVKVPLG
                     TKHKWQNDSNIESKVIFAVIL"
     gene            5541..6227
                     /locus_tag="KRL67_002957"
     CDS             5541..6227
                     /locus_tag="KRL67_002957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjjG family noncanonical pyrimidine
                     nucleotidase"
                     /protein_id="HBH1523823.1"
                     /translation="MKTYKFIFFDADDTLFDFKKSESHAFKKLLSEFDLEFNFENYIE
                     SYRNISDKLWLDLEKNIITLNELKLLRFELFANKISLDVDSETLSKMYLNFLGECTFL
                     ITGAIDILPYLKKKYTIAIITNGISAVQKKRLENSKIKGYIDGMVVSEELKISKPNPE
                     IFKYALKKFNCHDKSSALMIGDSLTSDILGGINSGIDTCWLNSNNSINYTNHIPTYEI
                     NTLIELKKLL"
     gene            complement(6351..6971)
                     /locus_tag="KRL67_002958"
     CDS             complement(6351..6971)
                     /locus_tag="KRL67_002958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH1523824.1"
                     /translation="MNYDSNKNKYLLEDINLEDIFVDKNIVLKFLGYGNRKAPDTISE
                     IVEREIRNINDILDIKIYISEVDINKTPKECKKAFAVLYTIGDKIDIKMNDYMENCNM
                     MAGLALDKIGIVCLDYINEKIKMYLKGKYDSLKISHEIYPGDKDFEVERQKDIYNYVK
                     NKYDSIEIEINDYHQLSPIKSVAMLILMGDEENLESRCSKCPRKCF"
     gene            complement(6961..7983)
                     /locus_tag="KRL67_002959"
     CDS             complement(6961..7983)
                     /locus_tag="KRL67_002959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HBH1523825.1"
                     /translation="MFSDMTLLQYINSEDRGFFLPDMGTNGLFLINKKAYEVYDSVDL
                     QLELAKTMDKYFESDFIYSFCDGITFCETLGLETLRPDYDFPSVLTHTITDRDKLRKL
                     DVPDPYTSGRMPLNIESLSAISKSIKKPLYESIQGPFTLAGQLAGATQLLRCIITDKK
                     FVEELLEFTTELVRRYAVAANKAGAKYISIAEPTSVTLSKDRFDEYIVKNLNKIYDEL
                     DCWKGMHICGDTRELLDNMLDCNIDAVSLDQILDYEEIAPMIPRDIVLIGNLDPIKLL
                     GRSKPDKIRRETLKLLKKMRGYDNFLCDFGCNCLNTTPVENLQAAIKAGRISYKELDR
                     IDIDEL"
     gene            complement(7984..8622)
                     /locus_tag="KRL67_002960"
     CDS             complement(7984..8622)
                     /locus_tag="KRL67_002960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423639.1"
                     /note="Presence of a B(12) (cobalamin)-binding domain
                     implies dependence on cobalamin itself, in one of its
                     several forms, or in some unusual lineages, dependence on
                     a cobalamin-like analog; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-dependent protein"
                     /protein_id="HBH1523826.1"
                     /translation="MDILDDIAECILNMGVDNIENLVKIAIDKNIDVEDIYEYGLNKG
                     MIGALDKFENKEYYLSEVIVCTDALNKGINLLKGTGKVKKKSKGVILMSVVEGDTHEI
                     GKNIVKVMVEATGYKVIDLGVNRKSEDIIEEAIKNNVDIIGLSSMMTTTMENMKSVID
                     KLNMIKIDKKPKVIIGGGPVSMEFAEEIGADGYSSNAPKAVKLINKLIGGEV"
     gene            complement(8740..9891)
                     /locus_tag="KRL67_002961"
     CDS             complement(8740..9891)
                     /locus_tag="KRL67_002961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004452985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBH1523827.1"
                     /translation="MELRPRSAKLEEFDKVIELINYVFRISRNHKPTMMEEFPLLLSK
                     NNIENMIIISEDDKVVSDVNYLIQDVSIQGNRLKVAAIGGVCTHPDYEKRGYSSKILD
                     KVEEKMFYNGVDIVIISGTRSLYSRRNCSLVKSFYKYTIKPEDVKIPYEIVEFDETNF
                     EKDDDLDKMIELYNQNSTRFIRTRDEFQKLLHAATIAWGTIAYKKVFIKENNNIIGYL
                     IIRTIQKEDSTVGEVAEIGLNSVNVENILKYVANKFGLEYLNYKVHVKNLKDQLKCNG
                     TKSLDYQQGTMKIINFTKLCDSLRSYFSQYVDSELLKYMEFKQVENKYIIKYKEEELV
                     IESLDKLNKLFFEKNEEQYNEFKHLKNIYEFATKAFPVDFPWTANLNYQ"
BASE COUNT         3265 a         1665 c          991 g         4028 t
ORIGIN      
        1 ttaattttta aaattataat aaaaaaagat aaacagaatt tttctgttta tctttttata
       61 cagtttgaga aatttttttc tcacttaatg agaagttttt ttctcactta atattatact
      121 ctgatatttt attggttaac tgtcttctac taatctccaa ttttttagct gtatttgaaa
      181 tattatttcc acacaaagat aaaacttttt caatatactc acattcaaac tcttttctta
      241 tttctctcaa aggtctaata cttattttat catctatata cacattattt gatattaaat
      301 ttaaattgtc ttttgataga ttgccttctt cacttaaaac tacaagtcta tttattatat
      361 tttttaattc tcttatattt cctggataat tgtaatttaa taaaaaatcc tttacttcct
      421 tatcaatcga gtgtatcttt ttatcatgtt caaattgata cttatttaag aaaaattcta
      481 taagagtagt taagtcttct cttctctttc ttagtggtgg tatagttatt gttattgtac
      541 ttattctata aaaaaaatct tctcttatat ttccatttga tatttccact tttggttctt
      601 tattcatagc acaaattaac ctaaagtcaa ctttaataga tttattactt cctatcctct
      661 ctatagattt gtcctcaagt gtcctcaaaa gttttacctg aacatttaaa ggtatatctc
      721 ctatttcatc taaaaataga gttcctttat tcgaaagttc aaatctacct tttctactat
      781 ctactgctcc tgtaaaagaa cctttttcat gaccaaacaa ctcagactcc aatagatttt
      841 ctgaaaaaga gcaacaattt acaggtacaa atatctcatt ctttcttgga cttatgctat
      901 gtatatatct agctaatata tcttttccta caccagattc gcccaagatt aatatattaa
      961 catctttaca agcagctttt tttgctattt ttatgatatt attaaaatct ctatttttag
     1021 attccaaagt aaattccaaa ttattttttt gtacagaaac agatatttta cttgccttta
     1081 ccttttcaac ttcttctaat aaattttcaa tgggattact ttttataaaa taagataaag
     1141 ctcctctttt catagcatca actgcatttt caatacttcc gtatcctgta actaatataa
     1201 cctctatatc tctattaatg gatttaattc tatctaacag ctgtactcca tccatatttt
     1261 tcatcataac atcagataaa atcaaatcat aatttttatc ttttttaatt atatttatag
     1321 cttcttcacc acttagtgct atatctacaa gatggccctt cttttgtaga atttttttca
     1381 ttaccactcc atattctagc tcatcatcaa ctactagtat tttcattata tagtcacctc
     1441 tttatttaat ggaattttta tatgaaaaca agttccaact cccaatttac tttcaacact
     1501 tatatctcca ttattctttt taatctcatt ataagtaata tataaaccta aaccagttcc
     1561 ctcaccaatt ggttttgttg tatagaaggg attaaatata tcctttaggg catcttcttt
     1621 tattccttca ccattatctt taaaatctat aaataaacaa tgattttttt cataacaata
     1681 tataataatt tttccatctt gatatattgc atctattgaa ttagaaatta aatttataaa
     1741 tacgtgtttt aaagattctc catttatata gcatactaaa ttatgttcac agtcaatttt
     1801 tatttcaaca ttctttaact gtaacatttt atattgaagt ttcactatat tttcaataaa
     1861 atttcttata tttatatgtt ctaaattatc atctgatatt cttgcgaaat ttaacaagtt
     1921 ggttattata ttacttgccc tatctacatt actttctatt gatttaacac aatcatttac
     1981 ttcttccatt gtaacgttat cttttaagag atagcaatta tttcttatta tacctaaagg
     2041 atttcttatc tcatgtgcta ccccagaagc taattgtcct atagatatca ttttattttc
     2101 tctaagtaat ttcgcctcta ctattttatc attagttata tctttaataa gaactactat
     2161 atactcagta ttattttttt tatactctac aggaaaagta tttatcttta aaattttatt
     2221 gtgtatttct atttcactag ttttattagt attttcagag aaagtatttt caataactcc
     2281 ttttatcatg ttaatcaaat ctgctatctt ataactcatt tcacccataa aatcatatag
     2341 tactttgttc gattctacta tattgttatt ttcatctaca agcattatta tatctgtaat
     2401 actattaaaa gttgcctcta aagttttttt attttctatt acctgttctg tttttctttt
     2461 tatttctatc tttaacaaat atgtatagct ataaaaaaca taaattgata ttaaaattat
     2521 acctataaaa agataaatac taggcacaat tcctcgctca tacaatatat catcaacttc
     2581 attatatgta gaataccatt ttttctttaa atctttatac actccatttt tctgaagttt
     2641 aaaaatacct ttatttaaaa tactaaccag ctcttcatat tgtttaggta cagcaagaac
     2701 tgctttttta gtatatatag gattacttaa aacgctgtat ttatttgaaa gtccataatt
     2761 atttatataa tatcgcaata ctggttcatc accaacaact gcgtctactt ttccagacat
     2821 taaatggttg actccagtct ttatatctgg agtcaataaa atgtttatat cttgaatttt
     2881 ttgctttaaa aaatttatag aataatcacc ttctggaatt gctactgttt tcccctttaa
     2941 atccatgtaa ctatttatat cttgggactt tttatctttc aatatatttg cactcaaagt
     3001 gtatattggg tcagtaaata taaatttatt tgccctttct tttgatggaa tcaagtcaaa
     3061 aaactttata ctatcatcta ttgaatttac gaaagattct ttccaccaat tatttggttt
     3121 aaaataaaaa tctctaccaa tttgaattga cagagaattt attaaatcaa caataattcc
     3181 cttatactgt ccatcttcct tacctttata tctcaaagga ggtgaacttt ggtcagagct
     3241 ataaatcaaa ttcttgtgtt tttctaacca ctttttttct tcttctgtaa atggaagtga
     3301 cttcttaata tattcaaata catttagatt gtactccata tttacataca tatttgtaag
     3361 tgaaaatact actaaaaatg aataaataat ccctataata acaatctttt ttttattcac
     3421 tttaaaactc caatcttctt ttttactata ataaattcaa tatttaataa caaaatccta
     3481 cttttttcat taaatattta tatttattat aaaactaatt gaatagatgt tgaaattata
     3541 gtcattacta ttcctatcaa tgcttcatat ggaattagct taaatctttc tcctataccc
     3601 atgtttatac tacctgcact agtatgaaat agtgagccat gtggtaattg ctcgaataca
     3661 ctagacccag catttactat tgatgcacct gagattggtg atagtccacc atttattata
     3721 gcatcatgaa aagtagatga tgctatagta gcaccagctg ttgatgatgc tgttgctaat
     3781 gacataagta tacccgaaac tggtgccaac aaaaattgtg gcatatttaa aaattgtaga
     3841 gcacctattg tactctgttg taattcagac atctgtataa ttcctgcgat agtccctgtt
     3901 ccaagtaata atatgcatac accttgcatc ttagataatc caaaagctaa atattctctt
     3961 gaattgttca agtttcctgt gacaattaga gttacgattc ctcctattgg aagggctatc
     4021 attgggtcta taactatagg agatacattt cctaaaaaca atagaaatat agctataatt
     4081 ggaccacata atgatttaaa taaacttggt aaatcttctc tttgctctaa gatttcatat
     4141 gattgtactt tatttccttt atttattaat atacttgcta aaataactgt aataacaact
     4201 ccaataattg ctggaattat atttgccatc ataacactag agagattgac agaaaaatta
     4261 tctgctactg ctatagtatt tggatttggt gatattatat tacctgcttt accccctccc
     4321 aacatggcta atagtattga caatttagaa taatttaatt ttctacctat atataatcct
     4381 ataggtgcta ccgttattat agatacatcc aaatttactc ctactccagc aagtaccata
     4441 gttgaaatga caatcgaaag taaagccctc ttttcaccaa atatttttac aatctgttct
     4501 gatattttat ctactgctcc tgtttttatt aaacttccag ctaatacacc tgatgccaaa
     4561 gctctaagaa ttgatggtga tatattcatg gctccatttg ccataaaatc tacagtttgt
     4621 gttatatttg ctcctccaac taatcctcca ataaatgctc ctaacattaa actatataca
     4681 gcttgaaatt tttttataat taaaataatt gctattaata aaccaaatat agctccaaaa
     4741 gtcgttattt gcataatatc ccctcctatc ataaattaac tatcttttac ttaattttat
     4801 ttaaatatat tttaagtatt ctgtatccac tttgtcaatt atattttaat ttatttaatt
     4861 atattgaaca tagttttttc aaaacctcat tctatatgtt ataataattt catactatat
     4921 aatcatttta cttgattatt ttaagttaat attttttaaa ttaaaaagag aggatattta
     4981 tatgaatgat ttaaatttag gggaaaaaat agctgaagtt cgaaaaaaac aaaacttaag
     5041 tattcgagat ttagcaaaac tagctgatgt tacaccatca ttattgagcc aaatagaacg
     5101 aggtcttgct aatccatctg taaattcatt aaagtctata gcctcttctc taaacgttcc
     5161 tttatttaca ttttttgtat ctgaagtaga taaaaaaaac ttgatagtaa gacataataa
     5221 tagaaaaaaa gttattttac ctggaagcaa agaagttata tatgagattt taattccaga
     5281 ctctagtggt aatttagaat ttgcaattat ggatttagct ccaaacactt cttcttgtgt
     5341 tgataggatt actcataatg gagatgaaat tgcatatgtt ttggagggag aagttaaact
     5401 ttttatggat gatgatgaat ttactttgtc caaaggagat agtgtaaaag ttcctttagg
     5461 aacaaagcat aaatggcaaa atgactcaaa tattgaaagc aaagtaattt ttgcagtcat
     5521 cttgtaaagg agtaaaaacc atgaagacat ataaatttat attttttgat gcagatgata
     5581 ctttatttga ttttaagaaa agtgaatcac atgcttttaa aaaacttcta tctgagtttg
     5641 atttagaatt taattttgag aattatatag agtcatatag aaatataagt gataagcttt
     5701 ggctcgacct tgaaaaaaac attataactt tgaatgaact taaattactt agatttgaac
     5761 tttttgcaaa taaaatatct ttggatgttg attctgaaac tcttagcaaa atgtacttaa
     5821 attttttagg tgaatgtacc tttttaatta caggtgcaat cgatatattg ccatatctta
     5881 aaaaaaagta tactattgct ataataacaa atggaatttc tgcggttcaa aagaaaagac
     5941 ttgaaaattc aaaaataaaa ggatatattg atggaatggt tgtatctgaa gagttgaaaa
     6001 tatcaaaacc aaatcctgaa atatttaaat acgctctcaa aaagtttaat tgtcatgata
     6061 aatcttctgc cttaatgatt ggggatagct taacctctga tatcttaggt ggaataaatt
     6121 caggaataga tacttgttgg ttaaattcca ataacagtat aaattatact aatcatatac
     6181 caacttatga aataaataca cttattgaat taaagaaact actatagttt gtaagtctgt
     6241 tcaaattaaa tagaatatta ctttgcaata atttttttag tttatacata aaatgaccaa
     6301 aaaaattatt gcaattcata cacaaaacaa atcttagtct tgaaaattaa ctaaaagcat
     6361 tttctaggac acttactaca tcttgattct aaattttctt catcccccat taaaataagc
     6421 attgcaacac tttttatggg ggatagttga tgataatcat tgatttctat ttctatacta
     6481 tcatatttat tttttacata gttataaata tccttttgtc tctctacttc aaaatcttta
     6541 tctcctggat atatttcatg cgatattttg agactatcat actttccttt taaatacatt
     6601 tttattttct cattaatata atctaaacat actataccta ttttatccaa tgctagccct
     6661 gccatcatat tgcaattttc catatagtca ttcatcttaa tatcaatttt atcccctatt
     6721 gtatatagta cagcaaatgc ttttttacat tctttaggtg ttttattaat atcaacttca
     6781 cttatatata tcttgatatc taaaatatca tttatatttc ttatttctct ttcaactatt
     6841 tcactaattg tatctggagc ttttctattt ccatacccca aaaactttag cacaatattc
     6901 ttatctacaa aaatatcctc taaatttata tcctctaaaa gatatttatt tttattacta
     6961 tcataattca tcaatatcaa tcctatctaa ttctttataa ctaattcttc ctgcctttat
     7021 agctgcttgt aaattttcta ctggagtggt atttaagcag ttacatccaa aatcacataa
     7081 aaagttatca tatcctctca tcttctttaa caattttagc gtctctcttc taattttatc
     7141 tggcttagaa cgtccaagaa gttttattgg gtctaaatta ccaatcaaca ctatatctct
     7201 aggaatcatt ggagcaatct cttcatagtc taaaatttgg tctaaactta ctgcatctat
     7261 attacaatct aacatattgt ctaataattc tctggtatct ccacatatat gcataccttt
     7321 ccaacaatca agttcatcgt agattttatt taaatttttt actatatatt catcaaatct
     7381 atctttactt agggttactg atgtcggctc tgctattgaa atatattttg ctcctgcttt
     7441 atttgcagct acagcatatc ttcttaccaa ttcagtcgta aattccaata attcttctac
     7501 aaatttttta tcagttatta tacatcttaa aagttgagta gctccagcta attgacctgc
     7561 aagtgtaaat ggaccttgta tcgattcata tagtggtttt ttaatacttt ttgatatagc
     7621 tgataagctc tctatattga gcggcatacg tccactcgta taagggtctg gaacatctaa
     7681 ttttcttaat ttatctctgt cagttatagt atgtgtcaat acacttggaa aatcataatc
     7741 tggtcttaat gtctctaaac ccagagtttc acaaaatgta atcccatcac agaatgagta
     7801 tataaaatct gattcaaaat acttgtccat agtctttgct aattctaatt gcaaatctac
     7861 actatcatat acttcatacg cctttttatt tattaagaat aatccatttg tacccatatc
     7921 aggcagaaaa aaacctctat cttcactatt tatatactga agtaaagtca tatctgaaaa
     7981 catttaaact tcacctccta taagtttgtt tattaattta actgcttttg gagcatttga
     8041 actgtatcca tctgctccta tttcttctgc aaactccata cttacaggac ctccaccaat
     8101 tattactttt ggttttttat ctatcttaat catattaagt ttgtctataa ccgatttcat
     8161 attctccatc gtagttgtca tcattgaaga taagcctatt atatctacat tgttttttat
     8221 agcttcttct attatatctt cactttttct atttacacct aagtcaataa ccttatatcc
     8281 agtggcttct accattactt ttacaatatt ttttcctatc tcatgagtat ctccttctac
     8341 tacagacatc agaattactc cctttgattt tttctttact tttccagttc ccttcaaaag
     8401 attaatacct ttatttaatg catctgtaca tactataact tctgaaagat agtattcttt
     8461 attctcaaac ttatctaaag ctcctatcat acctttgttt aaaccatatt cataaatatc
     8521 ttccacatca atatttttat ctatagcaat ttttactaaa ttctctatat tatctacacc
     8581 catatttaat atacattctg ctatatcatc aagtatgtcc atataatact catcctttct
     8641 aattacttac aaaattgctt tttcaatatc caataatttc tcttatttaa caaggactaa
     8701 ctctaggtat caatcaattt tatttaatat catctaaaac tattgataat ttaaatttgc
     8761 agtccatgga aaatctacag gaaatgcctt tgtagcaaat tcatatatat tttttagatg
     8821 tttaaattca ttgtactgtt cttcattttt ttcaaaaaac agtttattaa gcttatccaa
     8881 actttctatt acaagttctt cttctttata tttaataata tatttatttt caacttgttt
     8941 aaattccatg tattttaaca attcagaatc tacatattga ctaaaataac ttcttaaact
     9001 atcacacaac ttagtaaaat ttattatttt catagttcct tgttgatagt ccaaagattt
     9061 tgtcccatta catttcaact ggtctttaag atttttcaca tgtactttat aatttaaata
     9121 ttctaatccg aatttatttg caacatattt caatatattt tcaacattta cagagtttaa
     9181 ccctatttca gcaacttctc ctacagtaga gtcttctttt tgtattgttc ttattatcaa
     9241 atatcctatt atattattat tttcctttat aaataccttc ttgtaagcaa tcgttcccca
     9301 ggcaatagtt gctgcatgca atagtttttg aaactcatct ctcgttctta taaatctagt
     9361 actattttga ttatatagtt caatcatttt atctaaatca tcatcttttt caaaattagt
     9421 ttcatcaaat tcaactattt catatggtat ttttacatct tcaggtttta ttgtatattt
     9481 atagaaactc tttaccaaac tacaatttct tctactgtat agacttcttg ttcctgatat
     9541 aattacaata tctacaccat tataaaacat tttttcttct accttatcca atatcttaga
     9601 tgaatagcct ctcttttcat aatctggatg agtacatact ccacctatag ctgcaacctt
     9661 caatctgttt ccttgaatag atacatcttg aattagatag tttacatcag aaaccacttt
     9721 atcatcttct gatattataa tcatattttc tatattattt ttacttagta aaagaggaaa
     9781 ttcttccatc atagttggtt tatgatttct tgatatccta aatacatagt ttattaattc
     9841 tattacttta tcaaattctt ctaattttgc acttcttggt cttaattcca ttcatattcc
     9901 tccaaatatt atttaagata cttaaaattt tattgtctat aataaaaca
//
LOCUS       DAEPXF010000147         9748 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain HB27
            SAMN13376389-rid12479423.denovo.154, whole genome shotgun sequence.
ACCESSION   DAEPXF010000147 DAEPXF010000000
VERSION     DAEPXF010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13376389
            Sequence Read Archive: SRR10518432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 9748)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9748)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001069624.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001069624.1
            Genome Coverage       :: 315x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/15/2021 01:54:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,165
            CDSs (total)                      :: 4,076
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 89
            rRNAs                             :: 10, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 10 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 19 of 69
            Pseudo Genes (incomplete)         :: 44 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 10 of 69
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9748
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13376389-rid12479423.denovo.154"
                     /strain="HB27"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="China: Hebei"
                     /collection_date="2017"
     gene            complement(<1..2006)
                     /gene="recJ"
                     /locus_tag="KRL67_002962"
     CDS             complement(<1..2006)
                     /gene="recJ"
                     /locus_tag="KRL67_002962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HBH1523828.1"
                     /translation="MKKWILKYKGKIEESEFSKKLNISPEICQILKNRGICTEKDSEI
                     FMNPSLDYLRDPFLMKDMQKAVDRIKLAVEKNEKIWIYGDYDVDGVSSTSILCLYFKS
                     IGYNVDYYIPNRLEEGYGINEDAIKLISSRGCDLIISVDCGITSVSEVNTANDLGIDV
                     IITDHHECQSEIPSAFAVINPKQEDCNYPFDSLCGCGVAFKMIQALTPKEEFKTSMYN
                     YLEIVTLATICDIVPLIDENRIIVKNGLKSMKEGKNIGLRELIKVCGVESDKIGSSHI
                     GFAIGPRINASGRLGYSYLGVELFTTQSQEEAVEIASILEEKNNERQMIEAKMYHEAE
                     EMLKSNSRYNDDKVLVLAKEGWQHGIIGIVASKLTEKYYKPTILLGIENGEATGSARS
                     IKGFNIFEALIKCKDLMTKFGGHEQAAGLSLDSDNVEILANEINKFADYNLTEDDMIE
                     NVNVEFELQENVINLNLVEELHKLEPFGLNNPNPRFIVRNYILKDLKVIGKNQQHLKL
                     SIEKEKSYECIGFNMSHLKSMYKVGDKVDVLFQLDENNYMGNRKVQFLLKDIRLARPK
                     SASNDKLSLKLMSKIIPKDTQSLYNISVSDFELFDGNTDINIFDYFEKDTLIISNSIN
                     GFYRAMSDISLIDLDFNINYNIIEDVSKNTDKLELIFSPNIDKI"
     gene            complement(2019..3683)
                     /locus_tag="KRL67_002963"
     CDS             complement(2019..3683)
                     /locus_tag="KRL67_002963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/ABC1/UbiB kinase family protein"
                     /protein_id="HBH1523829.1"
                     /translation="MRISYRNLKRYREIGYVLIKYGFSFIVERLNIEGIAYKIPLFDP
                     PEEIKNMTTGERIKRVLEELGPTYIKIGQILSTRKDLLDQDIIDEISKLRDDVEKFDS
                     NIAIDIFKEEVGLSIEEIFLEFKEEPIAAASIGQVYEGVLRTGEEVIVKIQRPNIEKI
                     IKSDLEILRTIANTLKDLKKDFNLDLVQMIEEFQTQLMRELDYTFEAINATKFSRIFK
                     NSDEVYIPKVYSEYNTKKILVMEKVNGTKLSDVEKIRRLGYNTKTIVEIGVRSFFTQV
                     LSHGFFHADPHPGNIFVVAKNKIAYIDFGMIGIIDNKTLNQLNEIALAGVEKNVDKII
                     YLLIEMDALNGEADIKGLRQDLLYLIHYYYDISIEKINVTDILNELFRFFRQYKIVMP
                     AQFVTLAKTVITLEGTSRTLNTDFSFGSMGKEFMKHHYKSKFNPKNVVLSSRQNVEEI
                     LLDIKTIPKQLKAILKNIERNNIKMQIEDVKMTRLENCIIELTSQISLSLVLASIIVG
                     SSLIIASPNIENNIWIKFTAIAGFFISFIIGLCLVIRSIRSKYKKD"
     gene            complement(3981..4478)
                     /locus_tag="KRL67_002964"
     CDS             complement(3981..4478)
                     /locus_tag="KRL67_002964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HBH1523830.1"
                     /translation="MIRDYLEDKPLIDESVFVAKSADVIGNVKIGKDSSIWYNAVVRG
                     DEGPITIGENTNIQDCSIVHGDTETIIGNNVTVGHRSIVHGCKISDNVLIGMGSIILD
                     NAEIGEYTLIGAGTLITSNKKFPPGVLIMGSPGKVVRELTEEDKRYIDESYEWYLEAA
                     QNQKY"
     gene            complement(4507..5886)
                     /gene="scfB"
                     /locus_tag="KRL67_002965"
     CDS             complement(4507..5886)
                     /gene="scfB"
                     /locus_tag="KRL67_002965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioether cross-link-forming SCIFF peptide
                     maturase"
                     /protein_id="HBH1523831.1"
                     /translation="MSMIHKFSMNGYNIVLDVNGGAVHVLDDVAYDLLDFYKEKSKEE
                     ILEILKSKYQEEKINEAYEEILNLEKEGLLYTEDTYQYHPSFVHREPVVKALCLNVAH
                     DCNLKCKYCFAAQGDFGGEKELMSFEVGKAAIDYLIANSGSRKNLEIDFFGGEPLMNF
                     EVVKQLVDYGRSVEKDYNKNIRFTITTNGVLLNDEIIDYINENMHNVVLSLDGRKEVN
                     DNMRPTLNDKGSYDITLPRFKKLVEKRAKDKYYYIRGTFTRDNLDFSKDVMHFADLGF
                     KLTSVEPVVGDESNPYALREEDLPKIFEEYEKFAVEYADRQLQGDGFKFFHFMIDLNQ
                     GPCVIKRITGCGAGNEYLSVTPNGDIYPCHQFVGNEEFKMANIFDEEIVLPENLKNMF
                     REAHVYTKEECKQCWNKFYCSGGCHANAINFNNDISKPYELGCEMQRKRTECSIMIQA
                     KLMLEGATN"
     gene            complement(6191..6337)
                     /locus_tag="KRL67_002966"
     CDS             complement(6191..6337)
                     /locus_tag="KRL67_002966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic lactone autoinducer peptide"
                     /protein_id="HBH1523832.1"
                     /translation="MKKFIVRFMKFASSLALSTAILSANSTCPWIIHQPKVPKEISNL
                     KKTN"
     gene            complement(6367..6945)
                     /locus_tag="KRL67_002967"
     CDS             complement(6367..6945)
                     /locus_tag="KRL67_002967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory gene regulator B family protein"
                     /protein_id="HBH1523833.1"
                     /translation="MFKRYAEKMTSVLICNNMIDNNESKVYSYGFEILIAFIVNITTM
                     LFIGFLFGKFTYVLFFLMCYCPIRQFSGGYHADNYFRCLLTFIFIILSTILIIENLNI
                     DLFKNIIMIIASVSWVGICVLCPIEHRSNPISDREKLVYKKIAIFISTVVLLITILSL
                     SLSIFVDYFKYSAFAMFWIFVMLVLGKLKAKI"
     gene            complement(7313..8743)
                     /locus_tag="KRL67_002968"
     CDS             complement(7313..8743)
                     /locus_tag="KRL67_002968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4116 domain-containing protein"
                     /protein_id="HBH1523834.1"
                     /translation="MDIKNKIKSIFLPEKNDYLDDEEFEYDIENNEDDSKEDNKVHLN
                     DLSRVKYDYRELKDIENQTEEMCLLAVKQDGTIIEFVKDKTYKVCMEAVKQTYKALKY
                     ITNQNEDICIEAVKQNYRALYYINNKTENVLIEAIKNASTYDVMEVFKFVEEQTEDVC
                     LAFIERASKNDVAEILKGIKEQTPAICLEAVKKDGKSLAYVKEQSNSICLEAVKENYS
                     ALSCVKEQTEEICIEAVKQNDFALYYVNEQTEKICMEAVKRSYMALQYVNKQTEEICL
                     QAVRIDGRALQYVKEQTEEICLESVRQNGKVLQYVKKQTEDICIEAVRGSFEELEIKE
                     ILSYVKVPTERIFIEAVRQNGKILKYVESQTELMCLEAVRENYNALAYVKEQTEEICL
                     EAVNQSYEALKYVKEQTEEVCLKVVKQDYRMLKYINNQTEKICLEAVKQNYRALEFVD
                     NQTEKVCLEAVKQNRKALQYVKQKQS"
     gene            9087..>9748
                     /locus_tag="KRL67_002969"
     CDS             9087..>9748
                     /locus_tag="KRL67_002969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH1523835.1"
                     /translation="MKGNIVQYNFADIEEEVYSLDYAIAWNTDEENVNIIPFTNKFCK
                     ESIESFCLGKINNFVEILNEGFVENHHYVHLDKMISVPKKKVNLVYQQDTHGYLLRDD
                     NDKLIPAKITSDQSKSISSKMELFCAGEEKCLINILLKADPSYILDVDSIKDKNILNL
                     GYESIDRYKEYNFDDDKILIFFINKKRYSVIMKKTNNADNDLVSRNNTIKELFTNKAG
                     NL"
BASE COUNT         3159 a         1674 c          954 g         3961 t
ORIGIN      
        1 tctattttat caatattagg agaaaatata agctctaatt tatcagtatt tttgctaaca
       61 tcttcaataa tattgtaatt tatgttaaaa tccaaatcta taagtgatat atctgacatt
      121 gctctataaa atccatttat agaattagat attattagtg tatctttttc aaaataatca
      181 aatatattta tatctgtatt tccatcaaat aattcaaaat cagatacaga tatattatac
      241 aggctttgag tatcttttgg tattattttt gacattaatt tcaatgaaag tttgtcatta
      301 cttgcacttt taggtcgagc caatcgtata tctttaagta aaaattgtac tttcctattt
      361 cccatatagt tattttcatc taattggaac aatacatcta ctttgtctcc tactttatac
      421 attgatttta aatgactcat attaaatcct atacattcat agcttttctc tttttctatg
      481 cttaatttca gatgttgttg attcttacct atgactttca aatcttttaa aatataattt
      541 ctaactataa atctaggatt aggattattt aatccaaagg gttctaattt atgtaattct
      601 tctacaagat ttaaatttat tacattttct tgtaattcaa actcaacatt tacattctct
      661 atcatatcat cttcagttaa attatagtct gcaaatttat ttatttcatt tgctaaaatt
      721 tctacattgt ctgaatccaa agatagccct gctgcttgtt catgaccacc aaacttagtc
      781 atcaagtctt tgcactttat gagagcttca aatatattaa atccttttat tgacctcgct
      841 gaaccagtag cttccccatt ttctatacct agaagaattg ttggtttgta gtatttttca
      901 gttaactttg atgctacaat tcctattatt ccatgttgcc aaccctcttt agctaaaacc
      961 aacactttgt catcattgta tctgctatta gactttagca tttcttcagc ttcatggtac
     1021 atcttagctt ctatcatctg tctttcatta tttttttctt ccaatatact agctatttct
     1081 actgcctctt cttgtgattg agtagtaaat agctctactc ctaagtaaga atatcccaat
     1141 cggcctgaag catttatcct aggacctatt gcaaaaccta tatgagaaga acctatctta
     1201 tcactttcta caccacaaac ttttataagc tcccttaatc caatattttt gccttctttc
     1261 attgatttta aaccattttt tacaattatt ctattttcat ctataagtgg aacaatatca
     1321 caaatagttg ccagtgttac tatttccaaa taattataca tactagtttt aaattcttct
     1381 tttggagtca aggcttgaat cattttaaat gctacaccac atccacatag tgaatcaaat
     1441 ggataattac aatcttcttg ttttggattt attactgcaa aggctgatgg aatttcactt
     1501 tgacattcat gatggtccgt aattataaca tctatgccca aatcattcgc tgtattgacc
     1561 tcagacactg atgtaattcc acagtcaaca cttataatca agtcacatcc cctagagctt
     1621 ataagcttaa tagcatcttc atttatacca tacccttctt ctagtctatt aggaatgtag
     1681 taatcaacat tgtaacctat actcttaaaa tataagcaca agatagatgt agatgataca
     1741 ccatcaacat cataatctcc gtaaatccaa attttttcgt tcttctcaac tgctaacttt
     1801 attctatcaa cagctttttg catatctttc attaaaaaag ggtctcttaa ataatctaaa
     1861 gatggattca taaatatttc tgaatctttt tcagtacata tccctctatt ttttaaaatt
     1921 tggcagattt ccggggaaat attgagtttt ttgctaaatt cactttcctc tatcttacct
     1981 ttatacttca atatccattt tttcaaattt aattccccct aatctttttt gtattttgac
     2041 ctaatacttc ttataactaa gcaaaggcct attataaagg atataaagaa acctgctatt
     2101 gcagtaaatt ttatccatat attattttct atatttggag atgcaattat aagagaagaa
     2161 cctactataa tagatgccaa cactaaactc aaagatattt gagatgttaa ctctattata
     2221 caattttcta acctcgtcat tttaacatct tcaatttgca tttttatatt atttctttct
     2281 atatttttta aaattgcttt taattgcttt ggaatagttt ttatgtccag taatatttct
     2341 tctacatttt gtcttgaact taaaacaaca tttttagggt taaatttact tttataatga
     2401 tgtttcataa actcttttcc catagaacca aaagaaaaat ctgtgtttaa agttctactt
     2461 gttccttcta atgttataac tgtctttgca agagttacaa attgtgcagg catgactatt
     2521 ttgtactgtc taaaaaatct aaatagttca tttaatatat ctgttacatt tattttttct
     2581 atagaaatat catagtaata atgtattaga taaagtaaat cctgtctaag tcccttaata
     2641 tcagcctctc catttaatgc atccatttct atcaatagat atattatctt gtcaacattc
     2701 ttttctacac ctgctaatgc aatttcattt aattggttta aagttttatt atcaataatt
     2761 ccaatcattc caaaatctat atatgctatc ttatttttag caactacgaa tatattacct
     2821 ggatgtgggt ctgcatgaaa aaatccatga gataatacct gtgtaaaaaa tgacctaact
     2881 cctatttcaa caatagtttt tgtgttataa cctagtctcc ttattttttc tacatcactt
     2941 aactttgtac cattaacttt ctccattact aaaattttct tagtattgta ttctgaatat
     3001 actttaggta tatatacttc atcactattt ttaaatatcc tagagaattt agttgcattt
     3061 atagcttcaa atgtataatc aagttccctc attaattgag tttgaaattc ttcaatcatt
     3121 tgtactaaat ctaaattaaa atcttttttt aaatctttta aagtatttgc aatagttcta
     3181 agaatctcta aatcagattt tattattttc tctatatttg gtctttgaat ttttacgatt
     3241 acttcttctc cagttctaag tacaccttca taaacttgac caattgaagc tgctgctatt
     3301 ggttcttctt taaattctaa gaatatttct tctatactta aacccacttc ttctttaaat
     3361 atatcaatag ctatatttga atcaaatttc tctacatcat ctctgagttt tgatatttca
     3421 tcaattatat cctggtctaa caagtctttt cttgtactca atatctgtcc tatttttata
     3481 tatgtaggtc ctagttcttc taaaactctt tttattctct caccagttgt catatttttt
     3541 atttcttctg gtgggtcaaa tagtggtatc ttataggcaa ttccttctat atttagtctt
     3601 tctacaataa aactaaaacc atactttatt aaaacatatc caatttctct atatcgcttt
     3661 aaatttctat aacttattct caaatctttt cactcctaag ataggtatac ccaaaaactt
     3721 acaccttaat ttatcgtata tgacattatt tcaccaattg atattctaat atcttatatc
     3781 ctctttaata tttaatttta tcacatatac taccaatttt attaaaatta tttaactcag
     3841 ttaattttta tttttgacat tttatacctg gtttaatcaa atactttttg aaaactaaaa
     3901 tatcaacaaa atctaataaa ataaattagt ggttgcctca aaacagattt aaatatgttt
     3961 tgaaacaacc actctttaaa ctaatatttt tgattttgtg ctgcctctaa ataccattca
     4021 taagattcat caatatatct tttatcttct tctgtaagct ctctaacaac cttaccagga
     4081 gaacccatta tcaaaacacc tggtggaaac ttcttattag atgtaattaa tgtacctgct
     4141 cctattagag tatattctcc tatttcagca ttatccaata ttattgaacc cataccaatt
     4201 aacacattat cactaatttt acaaccatga actatcgacc tatgcccaac tgttacattg
     4261 ttaccaatta tagtttctgt atctccatgc actatagaac aatcttgtat attagtattt
     4321 tctccaatag ttattggtcc ttcatcaccc ctaactactg cattgtacca tatacttgaa
     4381 tctttgccta ttttaacatt tccaattaca tccgctgatt ttgctacaaa tacactttcg
     4441 tcaattaaag gtttatcttc taaataatct ctaatcatat ataaacaact cctttaaaat
     4501 ataaatttag ttggttgctc cttctaacat taacttagct tgtatcatta tagagcactc
     4561 tgtacgtttt ctttgcattt cacagcccaa ttcatatggt tttgatatgt cattattaaa
     4621 gtttatcgca tttgcatgac atccacctga acagtaaaac ttattccaac attgtttaca
     4681 ctcttctttg gtatatacat gagcttctct aaacatattt tttaaatttt ctggaagtac
     4741 tatttcttca tcaaatatat ttgccatttt aaattcttca tttccaacaa attgatgaca
     4801 tggatagata tctccatttg gagttacaga aagatactca tttccagctc cacatccagt
     4861 tattcttttt attacacatg gaccttgatt taggtctatc ataaaatgga aaaatttaaa
     4921 tccatctcct tgtaattgtc tatctgcata ctctacagca aatttttcgt actcttcaaa
     4981 tatcttaggt aaatcttctt ctctaagtgc atatggattg ctctcatctc caacgactgg
     5041 ctctacagag gttaatttaa atcctaaatc tgcaaaatgc ataacatctt ttgaaaaatc
     5101 taaattatct cttgtaaaag tacctcttat ataataatac ttatctttcg ctcttttttc
     5161 cacaagtttc ttaaatcgtg gtaatgtaat atcataactt cctttatcat ttaaagttgg
     5221 tctcatatta tcattaactt cttttcttcc atctaaactt aaaactacat tatgcatatt
     5281 ttcatttata taatctataa tctcatcatt taacagtaca ccatttgtag ttattgtaaa
     5341 tcttatattt ttattataat ctttttctac acttcttcca taatcaacca gttgttttac
     5401 aacctcaaag ttcatcaaag gttctccacc aaagaaatct atctctaggt tctttctact
     5461 tcctgaatta gcaatcaaat agtcaattgc tgccttacca acttcaaaac tcataagttc
     5521 tttttctcct ccaaaatcac cttgagctgc aaaacagtat ttacatttta agttacaatc
     5581 atgagctaca tttaaacata aagcttttac tacaggctct ctatgtacaa aacttggatg
     5641 atattgatat gtatcttctg tatataaaag accttctttt tccaaattca atatttcttc
     5701 atatgcttca tttatttttt cttcttgata ttttgatttt aatatctcta atatttcttc
     5761 tttacttttt tctttataaa aatctaacaa atcgtatgca acatcatcta acacatgaac
     5821 agctccacca ttcacatcta aaacgatatt atatccattc atagaaaatt tatgtatcat
     5881 actcataccc tacctcctaa attactatct atactaaagt tttttattat tttgattaaa
     5941 ctttaccctt cattatatca taagtttaca taaaatacat acaatacaaa aatgacttac
     6001 tctccgtaag tcatttcatt aaatacacta tatcccccta aattggcgca ctaatatatt
     6061 accaaataga tattaggctc ctataaaata aataaaataa attttgtatc tgattttata
     6121 ttgttttaat cttattaaaa tataataatt tctttttttc tgataatttt gtataaataa
     6181 gtgcttgtat ttaatttgtc ttctttaaat tgctgatttc tttgggtact ttaggctgat
     6241 gaataatcca aggacaggta ctattagctg acaaaatagc agtactaagt gccaatgagc
     6301 tagcaaactt cataaatctt acaataaact ttttcataac ttaactctcc ctcatacata
     6361 ttttacttat attttagctt ttaacttacc taatactaac atcacaaaaa tccaaaacat
     6421 ggcaaatgcg ctatatttga aataatctac aaatatactc aaagaaagac ttaaaattgt
     6481 tatcaataac actactgtag atataaatat agcaattttc ttataaacca acttctccct
     6541 atcacttata gggttgctcc tatgttctat tgggcataga acacaaattc caacccaact
     6601 aacacttgca ataatcataa taatattttt aaataagtct atatttagat tttctataat
     6661 taatattgtg gataaaatta taaaaataaa ggttaataag catctaaaat agttgtctgc
     6721 atgatatcct cctgaaaatt gtcttattgg acaataacac attaaaaaaa acaatacata
     6781 tgtaaatttg ccaaataaaa aacctataaa tagcatggtg gttatattaa ctatgaaagc
     6841 tattaatatt tcaaatccat aagaatagac tttagattca ttattatcta tcatattatt
     6901 acaaattaaa acagatgtca ttttttcagc atatctttta aacattttta cctccaaaaa
     6961 aattttacat atatagagta cattcttttt tgagaaaatt caatatactt tacaaaacta
     7021 gttgttaaaa atgtaaaact ggtcaaaaaa agacctatat aattttcttc ttattattta
     7081 tataacaaaa caattataat aaaacaattt tatcaagctt tatatgcata aaaaggcgat
     7141 atcaaaatag agataaatct ttttttcatt gttaaaaggc taaaaatata ttttatttca
     7201 tataaaaaga gtacctcaca aactaaaaag ttcattttga gatactcatt ttatttatta
     7261 caaaatatca atttttattt atttgcatat attttctata tattttggcc ttttaacttt
     7321 gtttttgttt tacatactgt agtgcttttc tattctgttt tactgcttca agacaaactt
     7381 tttcggtttg attatcaaca aattctaaag ctctataatt ttgctttact gcttctaaac
     7441 atattttttc ggtttgatta tttatatatt tcaacattct ataatcttgt tttactactt
     7501 ttagacaaac ttcttctgtt tgttctttca catatttaag cgcttcataa ctttgattaa
     7561 ctgcttctaa acaaatctct tctgtttgtt cctttacata agctaaagca ttatagtttt
     7621 ctcttactgc ctctaagcac attaattctg tctgactttc tacatattta agaatttttc
     7681 cattttgcct tactgcttct ataaatattc tttctgttgg tacctttaca taacttaata
     7741 tttcttttat ttcaagttct tcaaatgaac ctcttactgc ttctatacat atatcttcag
     7801 tctgtttttt tacgtattgc aatacttttc cattttgtct aactgactcc aagcaaattt
     7861 cttctgtttg ctcttttacg tactgtagtg ctcttccatc aattcttact gcttgcaaac
     7921 aaatctcttc tgtttgctta tttacatact gtagagccat ataacttctt tttactgctt
     7981 ccatacatat tttttctgtc tgttcattta cataatacaa tgcaaagtca ttctgtttta
     8041 ctgcttctat acatatttct tctgtttgct cttttacaca agatagtgca ctgtagtttt
     8101 cttttacagc ttctaaacat atactattac tttgttcctt tacataagct aaagattttc
     8161 cgtctttttt tacagcttct aaacatatag ctggagtttg ctcttttatt ccttttaata
     8221 tctctgcaac atcattttta gatgcccttt ctataaatgc tagacataca tcttctgtct
     8281 gctcttccac aaatttaaat acttccatga catcataagt tgatgcattt tttatagcct
     8341 caattagtac attttctgtc ttattattta tgtagtacaa agctctgtaa ttctgtttta
     8401 ctgcttctat acaaatatcc tcattttgat ttgttatata cttaagagct ttatatgtct
     8461 gttttactgc ttccatacat actttataag tcttgtcctt tacaaactca attatagtac
     8521 catcctgctt tactgctagc agacacatct cttctgtttg gttttctatg tcttttaact
     8581 ctctgtaatc atattttact ctgcttaaat catttaaatg tactttgtta tcttcttttg
     8641 aatcgtcctc attattttct atatcatact caaattcttc atcatctaaa tagtcatttt
     8701 tttctggtaa aaatatagat ttaattttat tctttatatc cataaaatta tcctccttta
     8761 atatgattct atcattaaag taaatcgttt ttaagcactt atttaattaa ttttatatta
     8821 agtttcttta aaagtctatt cattgttgat agattatatt ttatattaat acttttgtct
     8881 tatatatgtg gggtaataaa gttaatagtg gacattttcc atcccattag tatacactat
     8941 ttatatttat tactcattta tagtgatttt tttcaaattt tttcttattt actatatcat
     9001 atttatccaa tttatgatat tatttaaata taatataagt tttataaagt atattaaata
     9061 tattataaga tggaggtcga gtaattttga aaggaaacat agtacaatat aattttgcag
     9121 atatagagga agaagtatat tcgttggatt atgctatagc atggaataca gacgaggaaa
     9181 acgtaaacat aataccattt acaaataaat tttgtaaaga atctatagag tctttttgcc
     9241 taggtaaaat taataacttt gttgaaatat taaatgaagg ttttgttgaa aatcatcatt
     9301 atgtacattt agataaaatg attagtgttc ctaagaaaaa agttaattta gtatatcaac
     9361 aagacacgca tggttatctt ctaagagatg ataatgacaa gcttattcct gcaaaaataa
     9421 cttcagatca atctaaatct atatcaagta agatggagtt attctgtgct ggtgaagaaa
     9481 aatgtttaat taatatatta ctaaaggctg acccatcata tattttagat gtagatagta
     9541 taaaagataa aaacattcta aatctaggtt atgagtctat tgatagatat aaagaataca
     9601 attttgatga tgataaaatt ttaatatttt ttataaataa aaaacgttat tcagtgatta
     9661 tgaaaaaaac aaacaacgct gataatgatt tagtttccag aaataatact attaaagaat
     9721 tatttactaa taaagcagga aatcttaa
//
LOCUS       DAEPXF010000148         9675 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain HB27
            SAMN13376389-rid12479423.denovo.155, whole genome shotgun sequence.
ACCESSION   DAEPXF010000148 DAEPXF010000000
VERSION     DAEPXF010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13376389
            Sequence Read Archive: SRR10518432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 9675)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9675)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001069624.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001069624.1
            Genome Coverage       :: 315x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/15/2021 01:54:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,165
            CDSs (total)                      :: 4,076
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 89
            rRNAs                             :: 10, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 10 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 19 of 69
            Pseudo Genes (incomplete)         :: 44 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 10 of 69
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9675
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13376389-rid12479423.denovo.155"
                     /strain="HB27"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="China: Hebei"
                     /collection_date="2017"
     gene            957..2315
                     /locus_tag="KRL67_002970"
     CDS             957..2315
                     /locus_tag="KRL67_002970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine/pyrimidine permease"
                     /protein_id="HBH1523836.1"
                     /translation="MKAKSTSKFELDGIPPLKEAIPLGLQHLMAMVIGSCLPAILIAN
                     AAGLNHKMSTLLIQAALVFAGLSTLLQLYPIPLFKGFKLGGGIPVIMGATAMFIGAGV
                     SVASKYGLPVLFGSQIIAGIVIIIIGFGLKKIRFIFTPAVSGTIVACMGLGLFPIAIK
                     NLAGGMGNETFGNPINFIVGISVSLMILGLNKYGKGLFKDASILVSIIFGYILSLILG
                     IVNFSSIQEFTLVALPKPLAFGLDIKLEVVVMFSIIYLVEIADIMGACTLSAVGGLNR
                     QVTDEELSSAVLGNGVLSTIGALFSATPMGMFGQNSAIVSNTKVVSKFVLAIGGIGLF
                     LAGISPLLANLIRTIPPCVVGGATLVIFSTLTTSGLRLVSMDGFNQENSMILGLSMAS
                     GIGFMVAPQVLEKFPKFIETLLADSSVVSGAMVAIIIQALYMVKFPKNKSDKIEDKNK
                     SL"
     gene            2423..3217
                     /locus_tag="KRL67_002971"
     CDS             2423..3217
                     /locus_tag="KRL67_002971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HBH1523837.1"
                     /translation="MVTIKEYSVPQSLEEAYKILISKKNNVILGGCGFIKLSNKNIGT
                     AIDLKDINLNYIKEDEKNILIGADTSLRSLEIDKTIKNYCSGILSSAVSNIVGVQFRQ
                     GARIGASVFSKCGFSDLIPALLVVGAKVRLYNKGMLELSEFLDSELSRDILVEVILPK
                     KDAIAVFDSIRKCTGDFSVLNSAMLKENDTYKIAIGARPQKARLAFEASNILNKEKDI
                     DKASIVASKELTYGSNMRASKEYRKDMASALVKRMYNAIEEGMYNG"
     gene            3210..5516
                     /locus_tag="KRL67_002972"
     CDS             3210..5516
                     /locus_tag="KRL67_002972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HBH1523838.1"
                     /translation="MVNKGIRKIDSTAIVTGKPLYTEDLIMHKDVLTLKLLRSPHAHA
                     KIKNIDTSKAEKIPGVVGIYTYKDVPQVRYCVPGQTEPEASPYDRVLLEDTVRFIGDE
                     VALVAAEDEKTALKALKMIKVDYEVLKPVFDAREAEGNEVVIHPEKDIVEPYPFGLDA
                     KNNIVSKEFFEFGDVEAKLKESDFVVESSYKTQSQSHCMMETHRSYSYKDINDRIVVV
                     SSLQSPHNVKRILRKVLGIPSSKIRVIKPRIGGGFGGKNIAATDIFTAFVTMKTGRPA
                     KFVFTRSETFVGSNSRHEMFFDIKVGADKEGNLKVIELKSLNNTGAYGGNGVSVSSES
                     GHNTLPIYNDIDAVRFDARTVYTNRLPAGALRGYGATQGIFAIDSAIDELAHKIGMDP
                     LEFRIKNIIDRYTNGKAAEDEILSCELKQCIEKGKKLINWDDKYPCKDIGNNKVRAVG
                     MAVANHGSGIPRVDMATVTIRMDEDGTYKLLSGLADLGQGADTIQAQIAAEALNTTVD
                     RISIYTGDSDLCPYDTGAFASCTTTVTGNATIKTANKLKEILLSVAESKLGFKKEDLE
                     LGKDRVFVKNDNSKFVLLEELGRESVGGIGFSSDDVPVVLEATESFGMLKKIKPFIAG
                     FAEIELDKNTGEYKVINFACVPDCGTVINPTLARIQVEGGVMMGIGFAMYEDPKFDKD
                     GRLLTDSFLQYKVPTKKEVGNILVDFAENFDPGGPYGAKSLGEIVIHTPAPAIANAIF
                     NATGVRIRELPMTFEKVYMGMKNNNKEL"
     gene            5655..6932
                     /locus_tag="KRL67_002973"
     CDS             5655..6932
                     /locus_tag="KRL67_002973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBH1523839.1"
                     /translation="MPNRIKNIHYGTQRQQLKNLTKKEYQALREMCFLAKNLYNVGTY
                     NVRQHYFNQGEHLNYEANYHVCKENENYKLLNSNIAQQILKEVDSSFRSFFGLIKLAK
                     EGKYDFRGIKSPKYLDKEGFFSIIIGQIRIKEDGTLNVPMSPAFKKLYGKVSIKVPQN
                     ILEKKIKEIRIIPKHKARFFEIQYCYEIPKSEEVSNQKNALAIDLGLENLCTCVTNLG
                     DSFIIDGKRLKSVNQWANKQNDKIEALKLKKNITAITRKQFKVWNKRNNQVKDYVNKT
                     CFYIIDYCVKNNIGNLIVGYNAKLEKSDNMERKIYRNFIRIPFGEIKGKLEYLCKIKN
                     INFIRQEESYTSKSDFFADDFLPDINVDNVKKYDFKGERITRGQYKSSVGIVINADVN
                     ASLNILKKSQVVNLSNLQNNPQILKQPQRVRIS"
     gene            7115..7837
                     /gene="yqeC"
                     /locus_tag="KRL67_002974"
     CDS             7115..7837
                     /gene="yqeC"
                     /locus_tag="KRL67_002974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative selenium-dependent hydroxylase
                     accessory protein YqeC"
                     /protein_id="HBH1523840.1"
                     /translation="MELSNIIKKNEIITVVGAGGKTSFINYFANFYRDKLKVLLTTTT
                     KIYVPNDYDNIIITIDGTVIPSICHGITVCGSYINNENKLVSIDSSILDEIVDEFDLV
                     LIEGDGSKRKKLKGWNAKEPVVYHKTTKTIGILDITSFGMNINEENIHRVEIFKKIAN
                     LDTNSINSSSTVSIENLKNIVLNPNGLFKHYSGKRVLFINKVENEKYKNLAIKLIENI
                     KEYESEIEIFYGSVKQKFCARY"
     gene            7944..8513
                     /gene="mocA"
                     /locus_tag="KRL67_002975"
     CDS             7944..8513
                     /gene="mocA"
                     /locus_tag="KRL67_002975"
                     /EC_number="2.7.7.76"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor cytidylyltransferase"
                     /protein_id="HBH1523841.1"
                     /translation="MINAVIMASGFARRMGANKLLLDYKGCSIIEHVFRAVSKIDFHQ
                     VVVVSQYEEVLLLCNKYGFEYIDNKNANIGQSESIKLGVLNSSKCDGYMFFVGDQPFI
                     DNLYINKIINTFKLDRDFIVIPRYKDMCGNPVIFPYSKKEELLALKEDEKGKTILKNS
                     SKIKYVEVPKEMLFDIDTKVDYERIGGKL"
     gene            8510..9529
                     /locus_tag="KRL67_002976"
     CDS             8510..9529
                     /locus_tag="KRL67_002976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-binding protein"
                     /protein_id="HBH1523842.1"
                     /translation="MKLIKTIDAVGQVLCHDITQIIPGEFKGRKFKKGHIVKEEDIPV
                     LLSLGKDNLYVWEKSEGMVHENEGALFLKELTAGENLDFSEIKEGKIDFIAACDGLLK
                     IDVDALFDLNCVGEIMMATLHNNFIVNKGLKVAGTRVIPLIIDEKKLEDAKKVVGNRK
                     IVNVVPFKPKKVGIVTTGNEVFYSRIVDKFGPVIEEKVKGFGCEVIGQTICPDDKEII
                     KSAIKEFISQGAELICCTGGMSVDPDDVTPTAIKETGADLVTYGSPILPGAMFLLAYY
                     GEVPIMGIPGCAMYHKTTVFDIVLSRVLIDEKLDKYDIARYGHGGLCMNCDVCTYPAC
                     NFAKI"
BASE COUNT         3819 a         1013 c         1819 g         3024 t
ORIGIN      
        1 gaaacttgta tattttatgt tgcagataca aatatagtaa aggagagtac attggtacaa
       61 ctgagataca tagcttatta agtttttctg tcttatgaga attatattat aaaatattca
      121 taaatatttt gagtttaaat taaaaaatta ttttatgctt agattaattg atatttaatt
      181 aatttaataa caaaaacagt gaaagagtaa ataatattat ttacaggggg taaagcaaag
      241 tatgttaaat aaattgtact tttagccccg tacatagaga aatctatgta ggacacaact
      301 ttaaaaccag taaatcctaa agccttgcaa ctacaataaa agatagaaag aaacttttat
      361 gaatgtggtg aaaaccagaa acaatagtga ggatggtata tggtgagagc ctaagtactt
      421 aaaaatggac atttggtcgc caagcctaga aataggaagg tcaaacgact atctcgagag
      481 agagtacact ataagcgatt gatagtggaa acgggttgct cttagcaggt aatgctgaag
      541 atgaagaaat agtctacgct catatgaaag tatgaggtca ctggatttga agtgacatct
      601 ataaagtagc gtttataggt aaatgtttaa aacttgaaac atgtaaatac ttatgaaaat
      661 ttataactta taatgattaa gattgaaaga tttaaattga attaaattta ctataaaaaa
      721 ttaaaaattc atagctataa tactcttata tttgtaaagc attgtatata atgtatacaa
      781 aattaaagaa taggtttatt tgttaataat taaatttatt gcatctgttt tttaagacta
      841 aaaacttaaa catatgttgg agtatagttg atatttatga acatcattaa tttaatatat
      901 tagattttta agtatttata taattcaata agttttatgt aacgggagtt tttattatga
      961 aagcaaaaag tacatcaaaa tttgaattag acggaatacc accactaaag gaagcaatac
     1021 ctttagggtt acaacactta atggcaatgg taataggtag ttgtttacca gcgatattga
     1081 ttgcaaatgc agctggactt aatcataaga tgtcaacatt attgattcaa gccgcattag
     1141 tttttgcagg actatcaact ttattacaat tatatccaat acctttgttt aaaggtttta
     1201 aattaggagg aggaatacca gttataatgg gagctacagc tatgtttata ggagctgggg
     1261 tatctgttgc aagtaagtat ggactaccag tattatttgg ctctcaaata atagctggta
     1321 tagtaataat tataattggt tttggtctta aaaaaatacg atttatattt actccagctg
     1381 tatcaggaac aatagttgca tgtatgggtc ttggactttt tccaatagct ataaaaaact
     1441 tagctggggg tatgggcaat gaaacttttg ggaatccaat taattttata gttggaatat
     1501 ctgtatctct tatgatatta ggattaaata agtatggaaa aggattattt aaagatgcat
     1561 caatattagt tagcataatt tttggataca ttttatcatt aattttgggt atagtaaatt
     1621 tttcttcaat tcaagagttt actttggttg cacttccaaa accacttgct tttggtctag
     1681 atataaagct tgaagttgtt gtaatgtttt caataatata tttagttgag atagctgata
     1741 taatgggtgc ttgtacactt tctgcggtag gagggcttaa tagacaagta acagatgaag
     1801 aactttcttc tgctgtttta ggaaacggag tactctctac aataggtgca ttattttctg
     1861 caactccaat ggggatgttt ggacaaaatt ctgcaatcgt ttccaataca aaggttgtaa
     1921 gtaaatttgt actagctatt ggaggaattg gactattctt ggcaggaata tcacctctat
     1981 tagctaattt aataagaaca attcctccct gtgtggttgg aggtgcaaca ctcgttatat
     2041 tttcaactct tacaacatct ggtttaagac ttgttagtat ggatggattt aatcaagaaa
     2101 atagcatgat attaggtctt tctatggctt ctggtattgg attcatggta gctcctcaag
     2161 ttcttgaaaa atttccaaag tttatagaaa cattacttgc agattcaagt gtagtttcag
     2221 gagcaatggt agctataata attcaagctt tatatatggt aaaatttcct aaaaataaaa
     2281 gtgataaaat tgaggataaa aataaaagtt tataatgtaa aggtttaata tatgtgaatt
     2341 aaatatacta caattataaa tataaataaa aagatttatt aaaaactttt gtgttttaat
     2401 aagttaatga tggggggata agatggttac tataaaggaa tattcagttc cacagagttt
     2461 agaagaagca tataaaattt taatttctaa aaaaaataat gttattttag gtggatgtgg
     2521 atttataaaa ttaagtaata aaaatatagg aacagcgata gatttaaaag atataaatct
     2581 aaattatata aaagaggatg aaaaaaatat tttgataggt gcagatactt cacttagaag
     2641 tttagaaatt gataaaacta taaaaaacta ctgcagtgga atactttcaa gcgctgtttc
     2701 taatatagtt ggtgttcaat ttagacaagg ggctagaatt ggtgcaagtg tattttcaaa
     2761 gtgtggcttt tctgacttaa taccagcact tttggttgtt ggagcgaagg taagattata
     2821 caataaagga atgctagagt taagtgagtt tctggatagt gaattaagta gggatatatt
     2881 ggtcgaagta atactaccaa aaaaagatgc gatagctgtt tttgactcaa taagaaagtg
     2941 tacaggagat ttttctgtat taaattcagc aatgctaaaa gaaaatgata cttataaaat
     3001 agcaattggg gcaagacctc aaaaagctag attagcattt gaggcaagta atatattaaa
     3061 taaagaaaaa gatatagata aggcatctat tgttgctagt aaggaactta cttatggaag
     3121 taatatgaga gctagtaaag agtacagaaa ggatatggca agtgcattag ttaaaaggat
     3181 gtataatgct attgaggagg gaatgtataa tggttaataa ggggataaga aagatagata
     3241 gtacagcaat tgtcacagga aaacctctat atactgaaga cctcattatg cataaagatg
     3301 ttttgacatt aaaactactt agaagtccac atgctcatgc taagataaag aatatagata
     3361 catcaaaagc cgaaaaaatt ccaggagttg tgggtattta tacgtataaa gatgtaccac
     3421 aggttagata ttgtgttcca ggtcaaacag agccagaagc atcaccttat gatagagttt
     3481 tacttgaaga tacagttaga tttattggag atgaagtagc tttagtggct gctgaagatg
     3541 agaaaacagc acttaaagcc ttaaaaatga taaaggtaga ctatgaggtt ttaaagcctg
     3601 tgtttgatgc aagagaagca gaaggaaatg aagttgttat acatccagaa aaagacatag
     3661 tagaaccata cccttttgga ttggacgcaa aaaataatat agtatcaaaa gaattttttg
     3721 aatttggaga tgttgaagct aagcttaaag aaagtgattt tgtggttgaa tcttcataca
     3781 aaacccaaag tcaatcgcat tgcatgatgg aaactcatag gtcatattca tataaagata
     3841 taaatgatag aattgttgta gtttcatcac tacaatcacc acacaatgta aagagaatac
     3901 ttagaaaagt actgggtata ccatcttcaa aaataagagt aattaaacct agaataggtg
     3961 gtggatttgg aggaaagaat atagcagcaa cagatatatt tactgcattt gttactatga
     4021 aaactggtag accagcaaaa tttgtattta caagaagtga aacttttgta ggttctaata
     4081 gcagacatga aatgtttttt gatattaaag ttggtgcaga taaagaagga aatttaaagg
     4141 taatagagtt aaagtcatta aacaatacag gtgcttatgg agggaatggt gtatctgttt
     4201 cttccgaatc tggacacaac accttaccaa tatataatga tattgatgct gttagatttg
     4261 atgctagaac agtatatact aataggttac cagcaggagc acttagagga tatggtgcta
     4321 cccagggaat atttgcaata gatagtgcta ttgacgaatt ggcacataag attggaatgg
     4381 acccacttga atttagaatt aaaaatataa tagatagata tactaacgga aaagcagctg
     4441 aagacgaaat attaagttgt gagttaaaac aatgtataga aaagggtaag aaattaatta
     4501 attgggacga taaatatcca tgtaaagata taggaaataa caaagtaaga gcagtaggta
     4561 tggcagtggc aaatcatggt tctggtatac cacgtgtaga tatggcaaca gtgactataa
     4621 gaatggatga agatggcaca tacaagctat tatctggtct tgctgattta ggtcaaggag
     4681 ctgatacaat tcaagcacaa attgctgctg aagctttaaa cactactgta gatagaatat
     4741 ctatttatac aggtgattca gatttatgtc catatgatac aggagcattt gcttcttgta
     4801 caacaacggt tacaggaaat gcaacaataa agacagcaaa taaattaaaa gagatattac
     4861 tttcagttgc agagtcaaag ctaggattta aaaaagaaga tttagagttg ggaaaagata
     4921 gagtttttgt taaaaacgat aatagtaagt ttgttctatt ggaagaattg gggagagaat
     4981 ctgttggagg aataggattt tctagtgatg atgtgccagt agttttggaa gcaacagaat
     5041 cttttggcat gttaaaaaaa ataaaaccat ttatagcagg atttgcagag atagaattag
     5101 ataaaaatac aggcgaatat aaggtaataa attttgcatg tgtacctgat tgtggaacag
     5161 ttataaaccc aactttggct agaatacaag tagagggtgg tgtgatgatg ggtatagggt
     5221 ttgcaatgta tgaggaccct aaatttgata aagatggaag gttactaaca gatagtttct
     5281 tacagtataa agttcctaca aaaaaagaag tgggaaatat attggtagat ttcgctgaga
     5341 attttgaccc aggaggtcct tatggtgcta aatcattagg agaaatagtc atacatacac
     5401 cagcaccagc tatagcaaat gcaatattta atgctactgg agtccgtata agagagcttc
     5461 ctatgacttt tgaaaaagtt tatatgggta tgaaaaacaa taataaagaa ttataataag
     5521 taatatggta tataaaaaca tgtaaattta aattattttg taaaataaag taattaaatg
     5581 gacattatat atggtgtttt gtaaaaatac cgtatataat gtctagtaga gtatattcta
     5641 aaggaggtga gttcatgcca aacagaataa aaaatataca ctatggaact cagagacaac
     5701 aattaaaaaa cttaactaaa aaagaatatc aagctctacg agagatgtgc ttcttagcta
     5761 agaacttata taatgtcggt acatataatg tcagacagca ttattttaat caaggagaac
     5821 atcttaacta tgaagctaat tatcatgtat gtaaagaaaa tgagaattat aagcttctca
     5881 atagtaatat agcacagcaa atattaaaag aagttgacag ttcgtttagg tcattttttg
     5941 ggttaattaa attagcaaaa gaaggaaaat atgattttag agggataaag tcacctaaat
     6001 atttggataa agaaggtttt tttagtataa taataggtca aataagaatc aaagaagatg
     6061 ggactctgaa tgtaccaatg tctccagcat ttaaaaagct atatgggaaa gtaagtataa
     6121 aagttccaca aaacatacta gagaaaaaaa ttaaagaaat aagaataata cctaaacata
     6181 aagctaggtt ctttgaaatt cagtattgtt atgaaatacc gaaatcagag gaagtttcaa
     6241 atcaaaaaaa tgcactagct atagacttag gtttagaaaa tttatgtaca tgtgttacca
     6301 atttaggtga tagttttatc atagatggaa aaaggctcaa atctgtgaat cagtgggcaa
     6361 acaaacagaa tgataaaata gaagccttaa aattaaaaaa gaatataact gcaataacaa
     6421 gaaaacaatt taaagtttgg aataagagaa ataatcaagt taaagactat gtaaataaaa
     6481 cctgttttta tataattgat tattgtgtaa aaaataatat tggaaattta attgtaggat
     6541 ataatgctaa attagaaaaa agtgataata tggagagaaa aatatataga aattttataa
     6601 gaatcccatt tggagagata aaaggaaaat tagaatatct gtgtaaaata aaaaatatca
     6661 attttattag gcaagaagaa agctatacta gtaaatctga tttttttgct gatgattttc
     6721 tacctgatat caatgtggac aatgttaaaa agtatgactt taaaggggaa agaataacac
     6781 gtggacagta caaatccagt gttggaatag ttataaatgc agatgtaaat gcgagtctta
     6841 atatattaaa aaaatcacaa gtagttaatt tatctaattt acagaataat cctcaaatat
     6901 taaagcaacc acagagagta agaatatctt aattatattt aaaagataaa ctgatagcgt
     6961 aagcttacag cttagcggcg aattggatcc gccttgttgt ttacaacaga atctatttac
     7021 tttcgagttt atggaatttc aaaaatcact tttactaaaa aggtctagct ataataatta
     7081 agagattaga aaactggtta taagaggtga aagtgtggaa ctatcaaata taataaaaaa
     7141 gaatgaaata ataactgtag ttggagcagg aggaaaaact tcgtttataa actattttgc
     7201 aaatttttat agagataaat tgaaagttct ccttacaacg acaactaaaa tatatgtacc
     7261 aaacgattat gacaatataa ttattacaat agatggaact gttattccat ctatttgtca
     7321 tggaataaca gtatgtggca gttatataaa taatgaaaat aaattagtaa gtattgattc
     7381 ctctattttg gatgaaattg tagacgaatt tgatttagtt ttaatagagg gagatggctc
     7441 aaagagaaaa aaacttaaag gttggaatgc taaagaacca gtagtatatc ataaaactac
     7501 aaaaaccata ggtatattag atataacatc atttggaatg aatattaatg aagagaatat
     7561 acatagagta gaaatattta aaaaaatagc aaatttagat acaaatagta ttaatagtag
     7621 ctctacagta agtattgaaa acctaaaaaa tatagtatta aatccaaatg gattatttaa
     7681 acattattct ggaaaaagag tattatttat taataaagtt gaaaatgaaa aatataaaaa
     7741 tttagctata aaattaatag aaaatattaa agaatatgaa agtgaaatag agatatttta
     7801 tggaagtgta aaacaaaaat tttgtgcaag atattgaagt attaaaatag tttatatgat
     7861 attatatata tagataatat caatagagat attataaaaa tcatagaact atcaatatat
     7921 tataaatacc aaggatgata tatatgataa atgcagtgat aatggcatca ggttttgcca
     7981 gaagaatggg agcaaataaa cttttattag attataaagg ctgttctata atagaacatg
     8041 tatttagagc agttagcaag attgattttc atcaagttgt agtagtaagt caatatgaag
     8101 aggttttgtt attgtgtaat aaatatggtt ttgaatatat agataataaa aatgcaaata
     8161 taggtcaaag tgaatctata aaattgggag ttttaaatag ctcaaaatgt gatggttata
     8221 tgttctttgt aggagaccag ccatttattg ataacttata tataaataaa attataaata
     8281 cttttaaatt agatagagat tttatagtaa ttccaagata caaagatatg tgtggaaatc
     8341 cagtcatatt cccatatagc aagaaagaag aattgttggc acttaaagaa gatgaaaagg
     8401 gtaaaaccat tttaaaaaat tcatcaaaaa taaagtacgt agaagttcct aaagaaatgt
     8461 tgtttgatat cgatacaaaa gtggactatg aaagaattgg gggtaaatta tgaagctaat
     8521 aaaaactata gatgctgttg gacaagtttt atgtcacgac ataactcaaa taatacctgg
     8581 agaatttaaa ggaagaaaat ttaaaaaggg tcacatagta aaagaagaag atattccagt
     8641 gttattatct cttggtaaag ataatttgta tgtatgggaa aagtcagaag gaatggttca
     8701 tgaaaatgaa ggtgcattgt ttttaaaaga actaactgct ggagaaaatt tagattttag
     8761 tgaaataaaa gaaggaaaaa tagattttat agcagcatgt gatggtctat taaagataga
     8821 tgttgatgca ctttttgatt tgaattgtgt tggagaaata atgatggcta ctcttcataa
     8881 taattttata gtaaataaag gtttaaaagt agctggaaca agagtgatac ctttgattat
     8941 agatgagaaa aaattagaag atgcaaaaaa agtagttggt aatagaaaaa tagtaaatgt
     9001 agtgcctttt aaacctaaga aagtaggaat tgttactaca ggaaatgaag tattttatag
     9061 cagaattgtt gataaatttg gaccagtaat agaagaaaaa gtaaagggct ttggttgtga
     9121 ggttatagga cagactatat gcccagatga taaagaaatt ataaagagtg caataaaaga
     9181 atttataagt caaggagcag aacttatctg ctgtactggt gggatgagtg tagatccaga
     9241 tgatgttacg ccaacagcta taaaagaaac tggagctgat ttagtgacat atggttcacc
     9301 aatattacca ggggctatgt ttttacttgc ttattatgga gaagttccaa ttatgggtat
     9361 accaggatgc gcaatgtatc acaaaacaac agtatttgat atagttttat ctagggtttt
     9421 aattgatgaa aaacttgata agtatgatat agctagatat ggtcatggtg gcctttgtat
     9481 gaactgtgat gtatgtactt atcctgcatg taattttgct aaaatataaa gttattaaaa
     9541 aagagtattt acaaattaag tactcttttt aaatatgaaa caaacagcta gaatgatgtt
     9601 ttaagaaaag catattattt agaagttgta atagcttatt ttcaatgtgt aaaagaatga
     9661 ataaagttgc aattt
//
LOCUS       DAEPXF010000149         9639 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain HB27
            SAMN13376389-rid12479423.denovo.156, whole genome shotgun sequence.
ACCESSION   DAEPXF010000149 DAEPXF010000000
VERSION     DAEPXF010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13376389
            Sequence Read Archive: SRR10518432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 9639)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9639)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001069624.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001069624.1
            Genome Coverage       :: 315x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/15/2021 01:54:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,165
            CDSs (total)                      :: 4,076
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 89
            rRNAs                             :: 10, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 10 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 19 of 69
            Pseudo Genes (incomplete)         :: 44 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 10 of 69
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9639
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13376389-rid12479423.denovo.156"
                     /strain="HB27"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="China: Hebei"
                     /collection_date="2017"
     gene            66..1718
                     /locus_tag="KRL67_002977"
     CDS             66..1718
                     /locus_tag="KRL67_002977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding repeat-containing protein"
                     /protein_id="HBH1523843.1"
                     /translation="MKICKKLLTMGISLLLVISAFPIVSNGLSSIDNINGKDKYETSA
                     IIADRQNYTKAILINVDNSLADGLSASGLAGVENAPILLTKKDDIPDSTLKRLNNVKK
                     VYIIGGNNSIGSKVDNLLKKKHIEVERIEGKDRLSTSYKVSDKVLELKGTSGNILVAN
                     GFKGEADAISAASVAFKNAIPVLLTNGSDMPELKIKGDKIFAFGSTNTMSNQLVEKLG
                     ATRLGGIDRYETNKKIVQQFYGDAKEFYVASGSDLVYPLIGSTLAKSKPIVLVGNGSN
                     KSILKGATKITSIGNIEASIVTQCLNVTNNIGDTNTGVVKTNTNKEYPIKGMLAKLGL
                     NTTGKIGWDLNYGGNGNGVELRADGKYYYINRGNTALGAYAAALAGEKYHSLDFGDLD
                     PIEVIADKEEISYEKAQKEIVVIRNFLNSFDWQNASDLEKATRAGKLVTEADYVMGNY
                     NIYTNLLEKKSVCEGFAKSFYVLTRLMGMDSLYQEDGNLNHAWNYVKIDGKWYEFDGT
                     EAGSYKNLGIKVEFNSSKLEEATKQMPKYYDAKALSVLGFNQ"
     gene            2145..2666
                     /locus_tag="KRL67_002978"
     CDS             2145..2666
                     /locus_tag="KRL67_002978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBH1523844.1"
                     /translation="MDIAKLILNPARLRILQYIRLHSSVRTSDIVKYLNDIPRATVYH
                     HVKILEENNMIEVIEENRVRGTIEKVYALKEYTTSMEGETFVALSTAFYVGLMQEMNE
                     YFSRKEQDHKKDNVFFSSALLYMSDNEYENLLKSIADLLKPYIEQKPKSNLKLRKLSI
                     ISSPPVADNDKTK"
     gene            2700..3476
                     /locus_tag="KRL67_002979"
     CDS             2700..3476
                     /locus_tag="KRL67_002979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBH1523845.1"
                     /translation="MKFHVFGNKNRPHIMLIHGGGNAWWNYLRQAEVLSNNYHVILPT
                     LDGHGEEYETTYISTEDTADKLISYIDENCSGHLFALCGVSLGGQIVMELLSRKPNIT
                     KKAIIDGSICYPRPLMARFCIAGIRFFGGLLFSEKACRFQIAMMPKLLPENMQYPDEI
                     KAYYMTGMPHVRKETFYNMYRTYMMSYTLKENVKKTTAQVMYWYGEKEMKCVKKSAKM
                     FQSYVPSCEIYEAKGYNHGYLSLYLPNEWLEIAEPFFQKE"
     gene            3818..4771
                     /locus_tag="KRL67_002980"
     CDS             3818..4771
                     /locus_tag="KRL67_002980"
                     /EC_number="3.4.24.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GPR endopeptidase"
                     /protein_id="HBH1523846.1"
                     /translation="MISVRTDLALEASEMCEKSQEGSSIPGVKIETKELENCIVTKVE
                     VIDRQGAEIMNKDIGKYITLESNLMKFDDDESREEMISYLKEELVDIFGQDKNKKTLV
                     IGLGNRNITSDALGPKSVSKTLVTRHLFKNYNKDYDDDFTEVSALSPGVMGVTGIETS
                     EIVKSLVEKVKPDRVVAIDALASRKMERVNSTIQISTAGISPGGGVGNTRKSLTKETL
                     GVDVIAIGVPTVVDAATLTIDVLDMAIDNLIAQSEETESFYEMLKKLKEEEKYHLIKD
                     SLDPYDKNLIVTPKDIDDTIENLSIIISEGLNRSLHPGRLV"
     gene            4789..5808
                     /locus_tag="KRL67_002981"
     CDS             4789..5808
                     /locus_tag="KRL67_002981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein P"
                     /protein_id="HBH1523847.1"
                     /translation="MFKKCIKVVTLTFILACILPGKSLALNQDDFLKFLVNSSYPEAK
                     VEGNDTENKKNNKNKETSKENKEESKEGNTKSKDASKVDNKKESEKEYIKLYVGKENV
                     PDIESKNSDTTETNTTSSSDYKDDLRVTKENPRILIYHTHGCETYSNSPDGNYHSRDK
                     KNSVMEVGSALTSALDSKGWGVVHTTKYHDYPSYNNSYASSLKTIQSILPKYNSVDIA
                     IDLHRDARDLTNPATKEKDHLKYTTMINGERVSKFFFVVGGKNTNRKQLRALAEDITA
                     FAEKKYPGLVSPIVEKDYARFNQFAVKNHMLVEIGNNATSVEESKATTKYLAEILDEY
                     FKQKN"
     gene            5839..6090
                     /locus_tag="KRL67_002982"
     CDS             5839..6090
                     /locus_tag="KRL67_002982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH1523848.1"
                     /translation="MSRLEKNIESKSKLRKYKYIMRILFLITMIIGTSVCVFIVDSNA
                     KSMLGMKQYDFPSIKFDITKFDFNIPNLDIHEIITKFNK"
     gene            6372..8177
                     /gene="lepA"
                     /locus_tag="KRL67_002983"
     CDS             6372..8177
                     /gene="lepA"
                     /locus_tag="KRL67_002983"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HBH1523849.1"
                     /translation="MDNKQSRTRNFSIIAHIDHGKSTLADRLIQQTGLVSERDMKSQL
                     LDNMDLERERGITIKLQNIRLMYKAKDGNEYYLNLIDTPGHVDFNYEVSRSLAACEGA
                     LLVVDAAQGVEAQTLANVYLAIDQDLEILPIINKIDLPSARPEEVKNEIEDLIGLDSS
                     EAPLISAKTGLNIEDVLEDIVKNVPPPKGDNEAPLKALIFDSYYDAYKGVVAYVRVFE
                     GTVKKGMTIKMMNTNKKFEVTEVGVMAPGQTELSELSAGDVGYIAASIKDIRSCRVGD
                     TITDSNNPTEEPLPGYKKATPMVYCGIYPGEGEKYENVRDALEKLQVNDAALEYEAET
                     SAALGFGFRCGFLGLLHMEIMQERLEREFNLDIITTAPSVIYRVTKMDGEVVMIQNPA
                     NLPEPSEIKMIEEPIVKGDIIVPKDYVGVVMELCQERRGNMLNMEYIDERRVMLHYDL
                     PLNEVVYDFFDALKSRTRGYGSLDYEVKGYVASTLVKLDILINKEQVDALSFIVHETR
                     AFPRGKAMCEKLKGEIPRHQFAIPIQAAVGNKVIARETISALRKDVLAKCYGGDISRK
                     KKLLEKQKEGKKRMRQIGSVEVPQKAFMSVLKLDE"
     gene            8328..>9639
                     /locus_tag="KRL67_002984"
     CDS             8328..>9639
                     /locus_tag="KRL67_002984"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HBH1523850.1"
                     /translation="MINIEIPKKVDYIIKELETNGYEAYVVGGCVRDCLLERIPNDWD
                     ITTNARPEAVVELFKKTIPTGIQHGTVTVMIEHEPFEVTTYRIDGNYSDGRHPDSIEF
                     TNNIVKDLSRRDFTINSIAYNSKTGLVDPFNGYEDIQNKYIRCVGNPVDRFEEDALRM
                     LRAVRFSAQLNFKIAEGTKQSIHKKADLIKNVSIERIQTEFNKILVSDSSKLNLLKST
                     GLLKFIIPEICELEDVIQHNPYHIYDVQKHTLIATEVIEDELYLKLTMLFHDLGKKVT
                     KTTDKNGVDHFYTHSRESVKIAKKILRRLKYDNYTINKVLILIQYHDYRIEPKRKIIK
                     KLLNKLEDVELFEDLIKVNWADTLAKNPKYAKQKILNLIECEKEFKHIINQKECFNLK
                     DLAINGKDLISIGIKPGKDIGHILNKMLEIVINNPELNEKEILKE"
BASE COUNT         3831 a         1092 c         1750 g         2966 t
ORIGIN      
        1 tgattttttt caccttatag tatatcattt aagtggaact ataatatctg ggaggtattt
       61 atattatgaa aatatgtaaa aaattattaa ctatgggaat ttcattattg ctagtgattt
      121 ctgcttttcc aatagtatca aatggactta gttcgattga taatataaat ggtaaagata
      181 agtatgaaac ttcagccatt attgcagata ggcagaatta tactaaagct atccttatca
      241 atgtggataa tagtttagca gatggattat cagcaagtgg attggctgga gttgaaaatg
      301 caccaatatt attaactaag aaagatgata taccagattc aacattaaaa agacttaata
      361 atgttaaaaa agtgtacata ataggaggaa ataattctat aggaagcaaa gtagataact
      421 tattaaagaa aaaacatatt gaagttgaaa gaattgaagg aaaagataga cttagtacaa
      481 gttataaagt aagtgacaag gttttagagt taaaaggtac ttcaggtaat atactagtag
      541 caaatgggtt taaaggtgaa gctgatgcta taagtgcagc ctcagttgca tttaaaaatg
      601 cgatacccgt attacttact aatggttcag atatgcctga acttaaaatt aaaggagata
      661 aaatatttgc atttggttca acaaatacta tgagtaatca acttgtagaa aaattaggag
      721 caactagatt aggtggaatt gataggtatg agactaataa aaagatagtt caacagtttt
      781 atggtgatgc caaagaattt tatgtagcta gtggtagtga cttagtttat ccattaattg
      841 gctcaacttt agctaaaagt aaaccgattg tattagttgg aaatggaagt aacaaaagta
      901 tacttaaagg tgctactaaa ataacatcaa taggaaatat tgaggcttct atagtaacac
      961 aatgtctaaa tgtaactaat aatataggag atactaatac aggcgtagtc aaaaccaata
     1021 caaataagga gtatcctatc aaaggtatgt tagcaaagct tggtcttaac acaactggta
     1081 aaattgggtg ggaccttaat tatggaggaa atggaaatgg agttgaactt cgagctgatg
     1141 gaaaatatta ctatataaat aggggtaata cagcgttagg tgcatatgct gctgcattag
     1201 caggtgaaaa gtatcatagt ctggactttg gagatcttga ccctatagaa gtaatagcag
     1261 ataaagaaga gatttcttat gaaaaagctc aaaaagaaat tgtagtaatc aggaattttt
     1321 taaatagttt tgattggcaa aatgcttctg acttggaaaa ggctactaga gctggaaagt
     1381 tggtcacaga ggcagactat gtcatgggaa attataatat atatactaat ctccttgaaa
     1441 agaaaagtgt atgtgagggt tttgcaaaga gcttttatgt attgacaaga ctaatgggaa
     1501 tggattcact atatcaagag gatggtaatt taaatcatgc ttggaattat gtaaaaattg
     1561 atggtaaatg gtatgaattt gatgggactg aagctggatc atataaaaac ctgggaatta
     1621 aagtagaatt taattcttct aaacttgaag aggcaacaaa acaaatgcct aagtactatg
     1681 acgcgaaggc gttatctgta ttaggattta atcaataaaa gaaagactct ttaaagagtc
     1741 tttcttttat tttaaatatt ccaaaattat ttttaaattt gagtaattac tgttgactat
     1801 ttgatagttt tacataatgt acttaaatgg catagaaaat ttatttatat atttttataa
     1861 taaacagaaa atgaaaatag caatacaaat gttattccaa aagaaacttt aagtgttctg
     1921 gaattgaata aactgaaatt aaattacatt ggtatatatg ataataaata caccaaacaa
     1981 tataactaat atgttggaag attatatgca attaatgtca gtgtttatat agaatagcat
     2041 ttgtagcatt tattataatt gtaattacta tttatatcat agacttgaat agtctaaaaa
     2101 aatagactat aatattttta tagactaaag aaagggctga taagatggac atagcaaaac
     2161 taattttaaa tcctgcaagg cttcgtattt tacagtatat tcgacttcat agcagtgttc
     2221 gcacatctga tattgtaaag tatttaaatg atattcctcg tgcaacagtt tatcaccatg
     2281 taaaaatttt agaagagaac aatatgattg aagtaattga agaaaatcgt gtgcgtggta
     2341 ctattgaaaa ggtttatgca ctaaaggaat atacaacatc tatggaagga gagaccttcg
     2401 ttgcactttc tacagcattt tatgtaggtc tgatgcagga aatgaatgaa tactttagca
     2461 gaaaagagca agaccataaa aaggataatg tatttttctc ttcagcatta ctatatatga
     2521 gtgataatga atatgaaaat cttcttaaaa gtatagctga tttattgaaa ccatatatag
     2581 agcaaaagcc taaatcaaat ctaaaactta gaaaactttc aattatatct tctccacctg
     2641 tagctgataa tgataaaaca aaataacatg caccccattt ttataaaagg aggttatgta
     2701 tgaaatttca tgtgtttgga aataagaaca gaccacatat tatgttgatt catgggggag
     2761 ggaatgcatg gtggaactac ttgcgacaag cagaagtttt gtctaacaac tatcatgtga
     2821 tacttcctac attggatggt catggagaag aatatgaaac aacatacatt tctacagagg
     2881 atacagcaga taagttgata tcatatatag atgaaaattg cagtggtcat cttttcgctt
     2941 tatgtggggt atcacttggt ggtcaaattg taatggaact tttatctcga aaacctaata
     3001 ttactaaaaa agcaattatt gatggtagta tttgctaccc aagacctctt atggcaaggt
     3061 tttgtattgc tggtattcga ttttttggag gacttttatt tagtgagaag gcttgtcgct
     3121 ttcaaattgc tatgatgcca aaattgttac ctgaaaatat gcaatatcca gatgaaataa
     3181 aagcatatta tatgacaggt atgccacatg taagaaaaga aacattctat aatatgtatc
     3241 gtacttacat gatgagctac acacttaaag aaaatgttaa aaaaaccaca gcacaagtaa
     3301 tgtattggta tggagaaaaa gaaatgaagt gcgttaaaaa atctgcaaaa atgtttcagt
     3361 cttatgtacc ctcttgtgag atttatgaag ctaagggcta taatcatggg tatttatcgc
     3421 tttatcttcc aaatgaatgg ttggaaattg cggaaccatt tttccaaaag gaataaaggt
     3481 gttaggtgat tattgtataa ttattgctgt ttttttatgt agtaaataaa ttaggtatga
     3541 tataaaaaaa ttattttaat caatatatta gtgaaagctt aatatgaaaa ttataaatag
     3601 attaaaggta gcacctatat aaatagttgc tacctttttc tgtttgtaaa taaatgatag
     3661 ataagaaagt ttactgacaa atcactgact aaacccaatt ttcttacatt gaaagcctta
     3721 attttacttg ttttaaataa atttacaatt tcttgttata aaatactaaa ttttgttaac
     3781 aattataatt agcatttaat ttatactgga ggtttatatg attagtgtaa gaacagattt
     3841 agctttagaa gctagtgaaa tgtgtgaaaa gtctcaagaa ggaagtagta ttcctggtgt
     3901 aaaaatagaa actaaagaat tagaaaattg cattgtaact aaagttgaag taatagacag
     3961 gcaaggcgca gagataatga ataaagatat aggaaagtat ataactctgg aaagtaatct
     4021 tatgaagttt gatgatgatg agtcaagaga agaaatgata agttatttaa aagaggagtt
     4081 agtagatata tttggccaag acaaaaataa aaaaaccctg gtaattggtc ttggaaatag
     4141 aaatattact tctgatgcgt taggaccaaa gagcgtatca aaaacacttg taactagaca
     4201 tctttttaaa aattataata aagactatga tgatgatttc actgaggtat cagctttaag
     4261 cccaggagtt atgggtgtta ctggaataga gactagtgaa atagtaaagt cattggttga
     4321 gaaggttaag ccagatagag tagtagcaat agatgcattg gcttcaagaa aaatggaaag
     4381 agtaaactct actatacaga tatcaacagc aggaatatca cctggtggag gtgttggcaa
     4441 tactagaaaa tctctaacta aagaaacttt aggagtggat gttattgcaa taggcgttcc
     4501 aacggtagtt gatgcagcaa ctcttacaat agatgtctta gatatggcaa tagacaattt
     4561 aatagctcaa tcagaagaaa cagaaagttt ttatgaaatg ttgaaaaaac ttaaagaaga
     4621 agaaaaatat catctaataa aagattcgtt agacccatat gacaagaact taatagtaac
     4681 accaaaggat atagatgata caatcgaaaa tctatctata ataataagtg aaggcttaaa
     4741 taggtcttta catccaggta gattggtata aaataaggag gaacaattat gtttaaaaaa
     4801 tgcattaaag tagtaacctt gacttttata ttagcatgca ttttaccagg aaaatcatta
     4861 gctttgaatc aggatgattt tttaaagttt ttagtaaatt catcttatcc agaagctaaa
     4921 gtcgaaggaa atgatacaga aaataaaaag aataataaaa ataaagaaac aagtaaggaa
     4981 aataaggaag aaagtaaaga aggaaacact aaatctaaag atgcaagtaa ggttgataat
     5041 aaaaaagaga gtgaaaaaga atatataaaa ctttatgttg gtaaagaaaa tgttccagat
     5101 attgagtcaa aaaattcaga cacaactgaa actaatacaa catctagttc agattataag
     5161 gatgatttaa gagtaaccaa agaaaatcca agaattttga tatatcatac tcatggatgt
     5221 gagacttatt caaattcacc tgatggaaat tatcattcaa gagataagaa aaattcagta
     5281 atggaagtag gaagtgcatt aactagtgct ttagatagca aaggatgggg tgtagtccat
     5341 actactaaat accatgacta tccatcttat aataactctt atgcaagtag cttaaaaaca
     5401 atacaaagta tattacctaa atataactca gtggatatag cgatagactt acatagagat
     5461 gctagggatt taactaatcc agccactaag gaaaaagacc atttaaaata tacaactatg
     5521 ataaatggag agagagtatc aaagttcttc tttgttgtag gaggtaaaaa tacaaataga
     5581 aaacaactta gagctttagc agaagatata actgcttttg ctgagaagaa gtatcctggt
     5641 ctagtatctc caatagtaga aaaagactat gctagattca atcagtttgc agttaagaat
     5701 catatgttag tagaaatagg gaataacgca acaagtgtag aagaatctaa ggctactaca
     5761 aagtatttag ccgagatttt ggatgaatat tttaaacaaa aaaattagag ctttaaggta
     5821 taattaggag catgctaaat gagccgatta gaaaaaaata tagaaagtaa aagtaaacta
     5881 agaaaataca agtatattat gagaatctta tttttgatta ctatgattat aggtacatca
     5941 gtttgtgtat ttatagtaga cagtaatgca aaaagtatgc taggaatgaa acaatatgat
     6001 tttccaagta ttaaatttga tataacaaaa tttgatttta acataccaaa tcttgatata
     6061 catgaaatca taacaaaatt taataagtag taataagtga ataaggttta ttgattaaaa
     6121 taagccttat tttagttata tatttttaat gtataaaata aaaattcatt aactgtattt
     6181 tataggattt actttaagtt tacgatatta tataatattt agtgttagac ttaataatcc
     6241 acatataatg tggataattt tgtttagtgc caattagttt atattcaaaa attttaaatt
     6301 tggtatataa agagttttta gataatgtaa aataataact ataagaataa tcaagaggag
     6361 ggaattttaa ggtggacaat aaacaaagta gaactagaaa ttttagtata attgcacata
     6421 tagaccatgg aaagtctacc ttagctgata gattaataca acaaactggg cttgttagtg
     6481 aaagagacat gaaaagtcaa ttacttgaca atatggacct tgaaagagaa agaggaatca
     6541 ctataaagct tcaaaacata agattaatgt ataaagcgaa ggatggaaat gaatattatt
     6601 taaatcttat agatacacct ggacatgtag actttaacta tgaggtatca agaagtttag
     6661 ctgcatgtga aggagcacta ttagtagttg atgcagctca aggtgttgag gcacaaactt
     6721 tagccaatgt ttatcttgct atagaccaag atttagaaat attaccaata ataaataaaa
     6781 tagatttacc aagtgcaaga ccagaggaag taaaaaatga aatagaagat ttaatagggc
     6841 ttgattcaag tgaagctcct cttatttcag ctaaaactgg tttaaacata gaagatgttt
     6901 tagaagacat tgtaaaaaat gttcctccac ctaaaggaga taatgaagct ccattaaagg
     6961 cattaatatt cgattcttac tatgatgctt acaaaggtgt tgtagcttat gttagagtat
     7021 ttgaaggaac tgttaaaaag ggtatgacaa taaaaatgat gaataccaat aaaaagtttg
     7081 aggtaactga agttggtgtt atggctccag gtcaaactga acttagtgaa ttatcagcag
     7141 gtgatgttgg ttatattgct gctagtataa aagatataag aagttgtcgt gttggtgata
     7201 caataacaga ttcaaataat cctacagaag aacctttacc aggatataaa aaagctactc
     7261 caatggtata ttgtggtata tatccgggag aaggagaaaa gtatgaaaat gttagagatg
     7321 ctcttgaaaa gcttcaagta aatgatgctg cacttgaata tgaagctgag acttcagcag
     7381 cactaggatt tggttttaga tgtggtttct taggtctttt acatatggag ataatgcaag
     7441 aaagacttga gagagaattt aaccttgata taattactac agccccatct gttatatata
     7501 gagttacaaa aatggatgga gaagttgtaa tgatacaaaa tcctgcaaac ttaccagaac
     7561 catctgagat aaagatgata gaagagccta tagtaaaagg agatataata gttcctaaag
     7621 actatgtagg tgttgttatg gaactttgcc aggaaagacg tggaaacatg ttaaatatgg
     7681 aatatataga tgagagaaga gtaatgcttc actatgacct tcctctcaat gaagttgttt
     7741 acgatttctt tgatgcttta aaatcaagaa ctagaggtta tggttctctt gattatgagg
     7801 ttaaaggtta tgtagcatct acacttgtaa aactcgatat attaattaat aaagagcaag
     7861 ttgatgcact tagttttatt gttcacgaaa ctcgtgcttt ccctagagga aaggcaatgt
     7921 gcgagaaatt aaaaggggaa attccaagac atcagtttgc tatacctata caggctgctg
     7981 ttggtaataa ggttattgca agagaaacaa taagtgccct aagaaaagat gtccttgcta
     8041 aatgttatgg tggggatata tctcgtaaga agaaacttct tgaaaagcaa aaagaaggta
     8101 agaagcgtat gagacaaatt ggttctgttg aagttcctca aaaagcattt atgtctgtac
     8161 ttaaactaga tgaataaatt cttataaatt tagataaata tatttaaaag ctagagtgat
     8221 tttagaatta tttctaaatt aatctagttt ttttgtggta tattttaagt aaactcatag
     8281 agaatataag taagaatata ttaaagaaga ataaaaaggt gatatttatg attaatatag
     8341 aaattcctaa aaaagtagac tatataatta aagaattaga aacgaatggt tatgaagctt
     8401 atgtagttgg aggatgcgtc agagattgtt tattagaacg aatcccaaat gattgggata
     8461 taacaactaa cgctagacca gaagcagtag tagaattatt taaaaaaaca attccaaccg
     8521 gtatacaaca tggaactgta acagttatga ttgaacatga accatttgaa gtaacaactt
     8581 atagaattga tggaaattat agtgatggta gacatccaga ttcaatagaa tttacaaata
     8641 acatagttaa ggatttgtca agaagagatt ttactatcaa ttcaattgca tacaattcaa
     8701 agacaggatt agtcgaccca tttaatggct atgaagatat acaaaataag tatatacgtt
     8761 gtgttggaaa tccagtagat agatttgaag aagatgcact tagaatgctt agggcagtta
     8821 gattttcagc acaactcaat tttaaaatag ctgaaggtac taagcaaagt attcataaaa
     8881 aagcagattt gataaaaaat gtttctatcg aaagaatcca aacagagttt aataaaatat
     8941 tagtatctga ttcatctaaa ttaaacttat taaaatcaac aggtttgttg aaatttataa
     9001 ttcctgaaat atgtgaatta gaagatgtta ttcaacacaa tccatatcac atctatgatg
     9061 tacaaaaaca tactttaata gctacagaag taattgaaga tgaactgtat ttaaaactta
     9121 caatgttgtt tcatgattta ggtaaaaaag taactaaaac aactgataaa aatggtgttg
     9181 accattttta tactcatagt agagaatctg taaagattgc aaagaagatt ttgagaagac
     9241 ttaagtatga taattatact attaataaag ttttaatatt aatacaatat catgattata
     9301 gaatagaacc taaaaggaag ataattaaaa agttattaaa taaacttgaa gatgttgaat
     9361 tatttgaaga tttaataaaa gttaattggg ctgatacact tgccaaaaat ccaaagtatg
     9421 caaaacaaaa gatattaaat cttattgagt gtgaaaaaga atttaaacat ataataaatc
     9481 aaaaggagtg ctttaatctt aaagatttag ctattaatgg aaaagatttg atatctatag
     9541 gtataaaacc aggtaaagat ataggacata ttttaaataa gatgcttgaa atagtcataa
     9601 ataatccaga attaaatgaa aaagaaatac taaaagaaa
//
LOCUS       DAEPXF010000150         9565 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain HB27
            SAMN13376389-rid12479423.denovo.157, whole genome shotgun sequence.
ACCESSION   DAEPXF010000150 DAEPXF010000000
VERSION     DAEPXF010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13376389
            Sequence Read Archive: SRR10518432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 9565)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9565)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001069624.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001069624.1
            Genome Coverage       :: 315x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/15/2021 01:54:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,165
            CDSs (total)                      :: 4,076
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 89
            rRNAs                             :: 10, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 10 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 19 of 69
            Pseudo Genes (incomplete)         :: 44 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 10 of 69
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9565
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13376389-rid12479423.denovo.157"
                     /strain="HB27"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="China: Hebei"
                     /collection_date="2017"
     gene            <1..1305
                     /locus_tag="KRL67_002985"
     CDS             <1..1305
                     /locus_tag="KRL67_002985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional diguanylate
                     cyclase/phosphodiesterase"
                     /protein_id="HBH1523851.1"
                     /translation="KIKLQKIAYTDNLTGANTIDKFVIDANKILCKNTQVKYALLYID
                     IDKFKYINDLFGYEVGNEILRNLTKIIKSNTFEEEMFARISADNFIIIMKYIEEEDIT
                     KRLKTIFEELDMFNNNQEEKYKLVLSCGIYFILPEDRDINSIIDRANIPHKMAKGGHK
                     SSYAFYDNKIHDQEIKEKEMENTMFSSLENKEFIIYLQPKIELNTGEIQGSEALVRWK
                     RPDKGLIPPNEFIPFFERNGFVINLDLYVLEEVCIYLRKWIDAGINPVTVSVNVSRIH
                     LYCNNFIETYKNIIDKYNIPAKYIELELTESIIFDNFDILIDIMNNLKKIGFLISMDD
                     FGSGYSSLNMLKEIPMDILKLDQKFIMETYNSKRSKIIVTKVIEMAKELGMKVISEGV
                     ETEEQFELLKEVKCDMAQGYLFGKPMPIEEFEHLIVSNLVRG"
     gene            1480..1848
                     /locus_tag="KRL67_002986"
     CDS             1480..1848
                     /locus_tag="KRL67_002986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBH1523852.1"
                     /translation="MEWELDNNKPIYIQLVEHLKLKIISGEIKIGSKLETVRALAEDA
                     EVNPNTMQKALTELERQGLVYSQRTKGRFVTDDKEKIKAMKEEIANVEINTLKVTLEK
                     LGYDRDEMLKLITENLKGEL"
     gene            1850..2575
                     /locus_tag="KRL67_002987"
     CDS             1850..2575
                     /locus_tag="KRL67_002987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBH1523853.1"
                     /translation="MDKNINNVSQDGIVEFKGVNKSYGTKNVLKNIDLNIPKGKIVGL
                     LGPNGSGKSTMIKLMNGLLQPDNGEIMINGIKPSIETKKIVSYLPERTYLNDWMKVSD
                     LLKFFYDFYSDFDVRRANEMIKSLDIDVNEKLKTMSKGTKEKVQLILVMSRNASIYIL
                     DEPIGGVDPAARSYILKTILKNYSEDSTLLIATHLISEIENICDEVIFISKGEIVLQG
                     DVEAIREEKGKSIDALFREEFKC"
     gene            2569..3333
                     /locus_tag="KRL67_002988"
     CDS             2569..3333
                     /locus_tag="KRL67_002988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBH1523854.1"
                     /translation="MLGKLLKYELKASGRIFIPLYIAILIVAVFNGIFMNTNILQVQG
                     IGILVLTSLFMALGVLTIVVTIQRFRKNLLGDEGYLMFTLPVSTSSLILSKCITALIY
                     AVLSFIVAVFTFGVLMLFGTSGILLPEILDLFNTSFKWISENFLDILLLVVVMFISYS
                     SFILLLYTSISMGQLPKFNKHRNIVAFASFIAINIVISIVGDAVGSILPNEDTTMVYH
                     LYQSPSFMLAILGSLVVAIALFFATKFILDKKLNLE"
     gene            3555..4805
                     /locus_tag="KRL67_002989"
     CDS             3555..4805
                     /locus_tag="KRL67_002989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBH1523855.1"
                     /translation="MKSYTKAKWSVWGIITFSFVLVLFLRMSTAVVSDNLANELGFNS
                     IQISNIASFCLYAYAFMQIPAGILIDKYGARKISSLGIIMASLGSILFGLIQSIELAY
                     ISRVIVGAGTSVILLCILKIQGRWFNKSEFASATAKFSFVGNLGGVLATFPLVFLSEL
                     VGWRNSFLLIGIIGAVIGCFMYIIVRDTPKEYGFNVDTEPYEKSEKVNIVDGIKSVIK
                     NKSTWYNSMIMFSFVGLTSAFISLWGVRYIMDVYDVSKSFSAFIVSFFTYGFIFGSII
                     MDFVFAKIRSSKFNIIKFGAIIDLFIWIVIVVVYQVKPPIIVLPISFFIMGCIVMSHL
                     QVFNDAKYKNKEIYSGLATSVINTFEFIGSGIINLIIAISLQVNSYNIVDGYKKGFVV
                     FIVLSIITIVSSYIGVKNDDFKAI"
     gene            5011..5895
                     /locus_tag="KRL67_002990"
     CDS             5011..5895
                     /locus_tag="KRL67_002990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel"
                     /protein_id="HBH1523856.1"
                     /translation="MDTTKIDDISKLDLNNLDINKMDMGGIMESLLEWATTSGVKLII
                     GILILSIGFKIIKKFVNHVMLLLSKRDIDLTLRKFLKSLLLSVLKVAVIIIVLEYWGM
                     SLSSFAAVIASAGVAIGLALQGSLSNFAGGFIILLIRPFKVGDYVEAAGHGGTVEQIG
                     LFYTQLVTPDNKQILIPNGSVSNDSLINYSAKNTRRVDLTFSVGYEDDILHVRRVLKD
                     IVNNHKLIINEPEPFIGVVEHGDNAIKFAARVWCKTEDYWTIYLDLLEEVKVRFDEEG
                     ITIPYPKMDLTVKELNKI"
     gene            6255..6872
                     /locus_tag="KRL67_002991"
     CDS             6255..6872
                     /locus_tag="KRL67_002991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HBH1523857.1"
                     /translation="MCKKRYLLVVSIFIAGLLTACSGSPTTDSGQAQDNTKKEANASN
                     NALKDEKNNENLMEQDFKVPYTDAINIFKDKYKDADIVDLSLERDLNKFVYTVEGVDD
                     NNEYKMKIDANTKDVLEDKTEKLDSEDLNGVARKEKLDLNDIMTPQQAMEIALKEQNG
                     MVKEWSLDKDLDVTFYKIRIDKDKNEYDIKVDSKKGTVLKVEKED"
     gene            6980..9511
                     /locus_tag="KRL67_002992"
     CDS             6980..9511
                     /locus_tag="KRL67_002992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HBH1523858.1"
                     /translation="MSLKSLKIKENLYWVGSLDPDLRVFDIIMYTPYGTTYNSYVLKG
                     TEKTVLFETVKDKHFDNYIERLNDLNIDFEKIDYIVVSHTEPDHAGSVEKLLDLAKNA
                     KVVASETAIKYLKEIVNKDFEYVAVTDGDTLSIGDKTLEFFSVPMLHWPDTIYTYIKE
                     DKTLVTCDSFGSHYSNDKIVNTLDENEEKDYLDALRYYYDCIMGPFKPSMVTAIEKIK
                     DLDIDTVCPGHGPVLTENPRKIIDLYYNWSVNEQIKLEKEVTICYVSAHGYTKIMAEA
                     IKAYIEKNSNYKVNLFDVIEHKQEDILAKIAVSQGVLFGTPTILGDALKPIWDILVSL
                     NPVLHGGKVASVFGSYGWSGEGIENAMERISQLRMTAVKPFAVNFKPSNEEIDKLHSY
                     TGKFLDKLNSTFGSKKKTKKFKCVICNEVFEGDSAPSVCPVCGAKEDQFIEVEEDEVT
                     FRKDTDEYFVIVGNGAAGFYAADAIRKRNKTCKITMISNEDELTYYRPALSDGINEEL
                     GSDFYMEDKDWYDKNNIVVILGTNVDKLDEVNKTIIVNDGAIKFDKLVIATGSRNFIP
                     PIKGHDLENVFTLRNIKDLYSVKEALEKSKKVVVIGGGLLGLEAAWEFRLKGLEVVVV
                     EAMDSILSKQLDKEGSKILEQCVRDTGIDVRLGVAVDSIDGDGKAQKVVFKDGDSVDC
                     DMVVFSIGVRANTQMVQDTSVKIDRGIVVDKTLQTTVKDIYACGDVAQVGNTSLAIWP
                     SSVEMGKIAGANASGDNLTFESEVYPVSLDAMNVKVFSIGNIQNFDKEISSKDEGQRI
                     YKKLFMKDGSLVGAILINDLSCTVKLIRLISEKGDFEDIMKADIL"
BASE COUNT         3752 a          872 c         1734 g         3207 t
ORIGIN      
        1 aaaattaaat tgcaaaagat agcatataca gataatctta caggagcaaa tactatagat
       61 aaatttgtaa ttgatgctaa caaaatacta tgtaaaaata ctcaagtaaa gtatgcttta
      121 ttatatatag atattgataa atttaaatac ataaatgatt tatttggata tgaagttggg
      181 aatgaaattt tgcgtaattt aacaaagatt attaagagta atacatttga agaagaaatg
      241 tttgcaagaa tatctgcgga taattttatt ataattatga aatatataga agaagaagat
      301 ataacaaaga gattaaaaac tatatttgaa gaattagaca tgtttaataa taatcaagaa
      361 gaaaaatata agttagtttt aagttgtgga atctatttta tacttccaga agatagagat
      421 atcaactcaa tcatagatag ggctaatata cctcataaaa tggcaaaggg aggacataaa
      481 agttcatatg cattttatga taataagatt catgaccaag agataaaaga aaaagagatg
      541 gaaaatacaa tgttttcaag tctagaaaat aaagagttta ttatatactt acaacctaag
      601 atagaactta atacaggaga gatacaaggt tcagaagcac ttgttaggtg gaaacgtcct
      661 gataaaggac taatacctcc aaacgaattt atcccatttt ttgaaagaaa cggttttgtt
      721 ataaatttag atttatatgt acttgaagaa gtatgtatat accttagaaa atggattgat
      781 gcaggaataa atccagttac cgtttcagtt aatgtttcaa gaatacattt atattgcaat
      841 aattttatag aaacatacaa aaatataatt gataaataca atatacctgc aaagtatata
      901 gaacttgaat taacggagag tatcatattt gataattttg atattttaat tgatataatg
      961 aataatttaa aaaaaatagg atttttaata tctatggatg attttggttc agggtactca
     1021 tcattaaata tgttaaaaga aattcctatg gatatcttaa aacttgacca aaaatttatt
     1081 atggaaactt ataatagtaa aagaagcaaa ataattgtta ctaaagtaat agaaatggca
     1141 aaagagttgg gtatgaaagt catatcagaa ggagttgaga cagaagaaca gtttgaactt
     1201 ttaaaggaag taaaatgtga tatggcacaa ggatatttgt ttggaaagcc aatgccaatt
     1261 gaagaatttg aacatttaat tgtatctaat ttggtgagag ggtaattaat ctgtaatata
     1321 aagaatttaa taaattttgt taaaaatgga ctctaatgta gtgttcattt ttttgtaaaa
     1381 ttttttatac ttttattaaa aagtaattga catatacttt gtattattat attattgtat
     1441 taacaaatta atacaataat ataataagga ggcatgaaca tggaatggga acttgataat
     1501 aacaaaccta tatatataca attagtagaa catttaaagt taaaaataat ttcaggggaa
     1561 attaaaatag gatctaaatt agaaacagtg agagcactag cagaagatgc tgaagttaat
     1621 ccaaatacaa tgcaaaaggc tctaacagaa cttgaaagac aaggtttagt ttatagtcag
     1681 agaactaaag gaagatttgt aacagatgat aaagaaaaaa taaaagctat gaaagaagaa
     1741 atagctaatg tggagataaa tacattaaaa gtaactttag aaaagcttgg atatgataga
     1801 gatgaaatgt taaaacttat tacagaaaat ttgaaaggag aattataaaa tggataaaaa
     1861 tataaacaat gtttctcaag atggaatagt tgaatttaaa ggtgtaaaca agagttatgg
     1921 aactaagaat gttttaaaaa atattgattt aaatatacca aagggaaaaa tagtcgggct
     1981 gttaggacct aatggaagtg gtaaaagtac aatgattaag ctaatgaatg gattgttaca
     2041 gcctgataat ggtgaaatta tgataaatgg aattaaacca tccatagaga ctaaaaaaat
     2101 agtttcatat ttaccagaaa gaacttattt aaatgattgg atgaaggtat cagatttact
     2161 taagtttttc tatgattttt atagtgactt tgatgttaga agagcaaatg agatgattaa
     2221 aagtttagat attgatgtaa atgaaaagct aaagacaatg tctaaaggaa ctaaagaaaa
     2281 agttcaactt atcttagtaa tgtcaagaaa tgcaagcata tatatattgg atgaacctat
     2341 aggaggtgtt gacccagctg caaggtcata tatacttaag actatattaa aaaattattc
     2401 tgaagatagt actttactga ttgctaccca tttaataagt gaaatagaaa acatatgtga
     2461 cgaggtaata ttcatatcaa aaggagaaat agtgttacaa ggagacgttg aagcaataag
     2521 agaagaaaaa ggaaaatcta tagatgcatt atttagggag gaatttaaat gttaggtaaa
     2581 ttattaaaat atgagttaaa agcaagtgga agaatattta taccattata tatagccata
     2641 ttgatagtag ctgtatttaa tggaatattt atgaatacaa atatacttca ggtacaggga
     2701 ataggaatac tagtattaac aagtcttttt atggcattag gagtcttaac tattgtagtt
     2761 acaattcaaa ggtttagaaa aaatcttttg ggggatgagg gttatttaat gtttactctt
     2821 cctgttagca ctagttcact tatattatct aaatgtataa cagcacttat atacgctgta
     2881 cttagtttta tagttgcagt atttactttt ggagtactta tgctttttgg tacttctgga
     2941 attttactac ctgaaatttt agatttgttt aacactagtt ttaaatggat ttctgaaaac
     3001 tttttagata ttttattatt agtagtagta atgtttattt catattcatc atttattttg
     3061 ttattatata catcaatctc aatgggacaa ttgccaaaat ttaataaaca tagaaacata
     3121 gtggcatttg cttcttttat agctataaat atagtaatat caattgttgg agatgcagtt
     3181 ggaagtattt taccaaatga agatacaact atggtatatc atctttatca gagtcctagt
     3241 tttatgcttg caatacttgg tagccttgta gttgcaatag cattattttt cgctacaaaa
     3301 tttatattag ataaaaaatt aaatttagaa taaatgtttt tatatggagc catcttttga
     3361 gtaatgttaa aagatggctc ttgttattta tgtagttaat tcatgtatat aatataaata
     3421 aggttttatt tctagttaaa tatatacata tgttgtataa tgatattgcg tgtaaaaatt
     3481 tgtaagaata acgatacact ttatataagt tacatcaaga aaatttaaaa atatatggat
     3541 ttgagggaga agatatgaaa agttatacaa aggctaagtg gagtgtttgg ggtataatta
     3601 cattttcatt tgttttagta ttatttttaa ggatgtcaac tgctgttgtt tcagacaatt
     3661 tagcaaatga gttaggtttt aattctatac aaatttcaaa tatagcatcc ttctgcctat
     3721 atgcgtatgc atttatgcaa atacctgcag gaattttgat tgataaatat ggagcaagaa
     3781 aaattagcag tcttggaata ataatggcaa gtttagggtc aattttattt ggtttgattc
     3841 aaagtataga attagcatat atttctcgtg taattgtagg ggcgggaaca tcagtaatat
     3901 tactttgtat tttaaaaatt caagggagat ggttcaataa gtccgaattt gcttctgcaa
     3961 cagctaaatt ttcatttgta ggaaatttag ggggagttct tgctactttt ccacttgtgt
     4021 ttttgtctga acttgttggt tggagaaatt catttttatt aatcggtata attggtgctg
     4081 taattggatg ttttatgtat attattgtta gagatacacc aaaagagtat ggattcaatg
     4141 tagatactga accctatgaa aagtcagaga aggttaatat tgttgatggt attaagtctg
     4201 ttataaaaaa caaatcaaca tggtataatt caatgataat gttctctttt gtgggtctta
     4261 caagtgcatt tataagcttg tggggagtaa gatacataat ggacgtttat gatgtgagta
     4321 agagtttttc tgcatttata gtatcatttt ttacatatgg atttatattt ggttcaataa
     4381 ttatggattt tgtatttgct aaaataaggt ctagtaaatt taatataata aaatttgggg
     4441 caataataga tttgtttata tggattgtaa ttgtagttgt ataccaggta aaacccccaa
     4501 ttatagtttt accaatatca ttctttatta tgggatgtat agttatgtca catctacagg
     4561 tttttaatga tgccaagtat aaaaataaag aaatttattc tggactggca actagtgtta
     4621 ttaatacttt tgaatttata ggaagtggaa ttattaattt gattatagct atctctttac
     4681 aagtaaattc atataatata gttgatggat ataaaaaagg ttttgtagta tttatagtat
     4741 taagtattat aactatagta tcatcttata taggggtaaa aaatgatgat tttaaggcta
     4801 tatagtgttt tattaatagt ttaatttagt attatataac ttagtattat attttgcata
     4861 tatataacaa atagtttata ttttcctaag atgtataatt ggaatataaa tcttgtttac
     4921 tatgtacaaa tggtattgta ttatattaaa tagaaaataa gaactttaaa ctataatatt
     4981 ggttctaaaa tataaggtaa ggagtttttt atggatacga cgaaaataga tgatataagc
     5041 aagttagatt taaataattt agatattaat aagatggata tgggaggtat aatggaaagt
     5101 ctattagaat gggctactac aagcggagta aaacttataa taggtatact tatactttct
     5161 attggattta aaataataaa aaaatttgta aaccatgtta tgttattact tagtaaaaga
     5221 gatattgatt taactttaag aaagttttta aagtctcttc ttttgagtgt acttaaagtt
     5281 gctgttataa tcattgttct ggaatattgg ggaatgagtt tgtccagttt tgctgctgtg
     5341 atagcatcag caggggtagc aataggtttg gctttacaag gtagtctatc aaattttgct
     5401 ggaggattta ttatactatt gattagacct tttaaagtag gagattacgt agaagcagca
     5461 ggtcatggag gaacggttga acaaatagga cttttttata cacaattggt tactccagat
     5521 aataaacaga tacttatacc taatggttct gtatcaaatg atagtctaat aaactattca
     5581 gcaaagaata ctagacgagt tgatttaact tttagtgtag gatatgaaga tgatatacta
     5641 catgttagaa gagtacttaa agatattgtt aataatcata aattaataat aaatgaacca
     5701 gaaccattta taggagtagt agaacatgga gataatgcta ttaaatttgc agctagagtt
     5761 tggtgcaaga cagaagatta ttggacaatt tatcttgatt tactagaaga ggttaaagtt
     5821 aggtttgatg aagaaggaat tacgattcca tatcctaaaa tggatttaac ggtaaaggaa
     5881 ttaaataaaa tttaattttt taatatagtg tttaaattta agaatatcat atagatatgt
     5941 ttataagatt tgtaaaaaat taataagtac tgttattttg taagtaaaaa tatagaaaat
     6001 gggagtgtct caaaatgaac tttttagttc ctgaggcact ctttttgtat gaaatcaaat
     6061 atatttttat tatttaaaca ataaaaaaga ggcttgtttc tattttgaga caatatcatt
     6121 tttatataac tttaatctta gtaagtatga agttttaaaa ttactaaaat aggatgaaat
     6181 tttaatataa aagggtaaat aaagggttta gagggcataa tatagaataa attaattatt
     6241 taaggagggt ttttatgtgt aaaaaaagat atttattagt ggtaagtata tttatagcgg
     6301 gtttattaac tgcatgtagt gggagtccaa ctacggattc aggacaagca caagataata
     6361 caaaaaagga ggcaaatgca tcaaataacg ctttaaaaga tgaaaaaaat aatgaaaatt
     6421 taatggaaca agattttaaa gtgccataca cagatgcaat aaacatattt aaagataaat
     6481 ataaggatgc tgatatagta gatttaagtc ttgagagaga tttaaataaa tttgtttata
     6541 ctgtggaagg tgtagatgat aataatgaat ataagatgaa aattgatgct aatacaaaag
     6601 atgtattaga agataaaacc gaaaagttgg attcagaaga tttaaatgga gttgctagaa
     6661 aagaaaaatt agatttaaat gacataatga cacctcaaca agctatggaa attgcattaa
     6721 aagaacaaaa tggaatggtt aaagagtgga gtttagataa agatttagat gttacttttt
     6781 ataaaataag aatagataaa gataaaaatg aatatgacat aaaagtagat tctaaaaagg
     6841 gaactgtatt gaaggtagaa aaagaagatt agaagattta aactatcttt aaaataattt
     6901 cttttttgaa taaattttgt ttaaaatata acaaacaggg tataaagaga ctaaataact
     6961 attttaggag gagtacggta tgtcattgaa atcgctaaaa ataaaagaaa atttatattg
     7021 ggtaggttcg cttgaccctg atttaagagt atttgacata attatgtata caccttatgg
     7081 aactacttat aattcatatg tattaaaagg aacagaaaaa acagttttat ttgagacagt
     7141 taaagataag cattttgaca attatattga aagattaaat gatttaaata tagattttga
     7201 aaaaatagac tatattgttg taagtcatac tgaaccagac catgcaggaa gtgttgaaaa
     7261 gcttttggat ttagctaaaa atgcaaaagt agtagcttca gaaacagcaa ttaaatatct
     7321 taaggaaatc gtaaacaaag actttgaata tgttgcagtt acagatggag atacattatc
     7381 aattggggac aaaactttag aatttttctc tgtacctatg cttcattggc cagataccat
     7441 atacacatat ataaaagagg ataaaactct tgtaacatgt gattcatttg gtagtcacta
     7501 tagtaatgat aagatagtaa atacacttga tgaaaatgaa gaaaaggatt acttggatgc
     7561 tttgagatac tattatgatt gtataatggg gccatttaaa ccatctatgg taactgctat
     7621 agaaaagata aaagatttag atatagacac tgtatgtcca ggtcatggtc cagtattaac
     7681 tgaaaatcct agaaaaatta tagaccttta ttacaactgg agtgtaaatg aacaaataaa
     7741 gttagaaaaa gaagttacaa tttgctatgt ttctgcacat ggctatacaa agattatggc
     7801 agaagctata aaagcatata ttgaaaaaaa tagtaactat aaagtaaatt tatttgatgt
     7861 aatagagcat aagcaagaag atattttggc aaaaatagca gtatcacaag gtgtattatt
     7921 tggaacacca acaatattag gagatgcgtt aaagcctata tgggatatat tagtatcgtt
     7981 aaacccagtt cttcatggtg gaaaagttgc ttcagtattt ggttcatatg gatggagtgg
     8041 agaaggtata gaaaatgcta tggagagaat aagccagctt agaatgacag cagtaaaacc
     8101 ttttgctgtt aactttaaac catcaaatga agagattgat aaattacatt cttatacagg
     8161 taaattccta gataagttaa attctacctt tggaagtaaa aagaaaacta agaaatttaa
     8221 atgtgtaatt tgtaatgaag tttttgaagg tgatagtgct cctagtgtat gtcctgtatg
     8281 tggagctaag gaagaccaat ttatagaagt tgaagaagat gaagttactt ttagaaaaga
     8341 tactgatgaa tatttcgtaa tagtaggaaa tggagcagct ggtttttatg cagcagatgc
     8401 tataagaaaa agaaataaaa cttgtaagat aactatgatt tctaatgaag atgagttgac
     8461 ttactataga cctgcacttt ctgatggtat aaatgaagaa cttggttcag atttctatat
     8521 ggaagataaa gattggtatg ataaaaataa tatagtagta atattaggaa cgaatgtaga
     8581 taaattagat gaagtgaata aaactattat agtgaatgat ggagctatta aatttgataa
     8641 gcttgtaata gctacaggaa gtagaaactt tataccacca attaagggtc atgatttaga
     8701 aaatgtgttt actcttagaa atataaaaga tttatatagt gtaaaagaag cactagaaaa
     8761 atctaagaaa gttgtagtga ttggtggagg tttattggga cttgaagctg catgggagtt
     8821 tagactaaaa ggattagaag tagtagtagt tgaagcaatg gatagcatac tatcaaaaca
     8881 acttgataaa gaaggtagta aaatattaga acaatgtgta agagatacag gaatagatgt
     8941 aagattgggt gtagcagttg atagtattga cggtgatgga aaagctcaaa aagttgtatt
     9001 taaagatgga gatagtgtcg attgtgatat ggtagtattt tcaattggtg taagagcaaa
     9061 cactcaaatg gttcaggata catctgtaaa aattgataga ggtattgttg tagataagac
     9121 attacagaca actgttaaag atatatatgc ttgtggagat gttgcacaag tgggaaatac
     9181 tagtttagca atatggccat cttcagtaga aatgggaaaa atagcagggg caaatgcaag
     9241 tggagataat ttgacatttg aaagtgaagt atatcctgtt tcattagatg caatgaatgt
     9301 aaaagtattt agtattggta atatacaaaa ttttgataaa gaaatttcat caaaagatga
     9361 aggtcaaaga atatataaaa aattatttat gaaagatggt tcattggttg gagcaatact
     9421 tataaatgat ttaagctgta ctgttaaatt gataagatta atatctgaaa aaggtgattt
     9481 tgaggatatt atgaaggctg atatactata atttaaatat actaaaacaa aaagtcttaa
     9541 aatatgacaa ttcatatttt aagac
//
LOCUS       DAEPXF010000151         9533 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain HB27
            SAMN13376389-rid12479423.denovo.158, whole genome shotgun sequence.
ACCESSION   DAEPXF010000151 DAEPXF010000000
VERSION     DAEPXF010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13376389
            Sequence Read Archive: SRR10518432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 9533)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9533)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001069624.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001069624.1
            Genome Coverage       :: 315x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/15/2021 01:54:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,165
            CDSs (total)                      :: 4,076
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 89
            rRNAs                             :: 10, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 10 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 19 of 69
            Pseudo Genes (incomplete)         :: 44 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 10 of 69
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9533
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13376389-rid12479423.denovo.158"
                     /strain="HB27"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="China: Hebei"
                     /collection_date="2017"
     gene            120..791
                     /locus_tag="KRL67_002993"
     CDS             120..791
                     /locus_tag="KRL67_002993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBH1523859.1"
                     /translation="MNLLIIEDDINLNEGLFYAFENDGFNVFKAYTKQEGLNIFNSKN
                     IDFIILDCNLPDGDGFDVCQLIREKSDIPIIMLTARDSEIDEVKGLEIGLDDYITKPF
                     SLSVLKARVKVAIRKKSNNKVIYSNGIKLDQKLLKVYKNKVCLELSSVEYKLLSYLIE
                     NKGQILLKEQILHHIWDSEENYVDENIVSVNIRRLRVKVEDDPSNPKYIKTAYGMGYL
                     WNEVE"
     gene            793..1701
                     /locus_tag="KRL67_002994"
     CDS             793..1701
                     /locus_tag="KRL67_002994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBH1523860.1"
                     /translation="MFILAFIICTVVIFIVSTKINQKRYDELSLMLNQILEGKEVTYP
                     DTKDTRASKISQQVKKIKDMIEIEVEQSKLEKEAIKGLISNMSHQLKTPLSNITIYCE
                     LLENINISTLQKKEFLQKMKNETFKIDWLLQSLFKMTKLEDGVIEFEVEELLIKDTLI
                     QSISTIFNKAEAKNIRVNLEPFSDIKLVHNKKWTIEAIVNVLENAIKYSPSDSTITIS
                     VIKMELYTKITIKDEGIGIDSRELNDIFKRFYRSKNVENQNGTGIGLYLTRLILEKEN
                     GNIIVESKLGSGCCFSIFLQNCKSLN"
     gene            1776..2462
                     /locus_tag="KRL67_002995"
     CDS             1776..2462
                     /locus_tag="KRL67_002995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBH1523861.1"
                     /translation="MSILVTNNLVKHYGKDETKVKALNGVSIEIERATFTAIVGTSGS
                     GKSTLLNIIGGLDSPTSGDVIIKGKNISKIGKKDLTVFRRRNIGFIFQNYSLMPVLNT
                     YDNIALPVTLDKGNHIDHEYIEMLMKTLGIWDKRLKFPSELSGGQQQRVAIARALANK
                     PALILADEPTGNLDSKTTMEVVCLLKESSAKFHQTILMVTHNENIAQVCDSIIHIEDG
                     VVVNTGGEVL"
     gene            2459..5014
                     /locus_tag="KRL67_002996"
     CDS             2459..5014
                     /locus_tag="KRL67_002996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HBH1523862.1"
                     /translation="MMSLVKFSIQYIKHYKKQSISIVLSIVLSVALLTGIGSLVHSAD
                     KSRIEKIREDSGDYHYFYKVDKEQLNKIKENKKSKEYTVDRLGVTKPKNQIDEPFIIE
                     FLNADSSYLDMTGRKLLKGTLPSKDGEIVLDTYSLNNLNVNKEIGTEINLDGKIYKLC
                     GILSSLSNPETAMEGFVNDYLQPKEKDSYIVYVKYDENKNIKKQNYKFMKKFNISKEK
                     AYINWPLSVEFGVKPPKNIEKYNIFEFLKNLELNTNAIILLVGVFGAAAVYSIFHVSI
                     LQRISQYGVLEVLGANNKQLLFLLFLELFLLFIVGFPIGCFLGIGVASTIYEQFPHIF
                     LSSDIVPGAFFISEKSIYLGFLFILLLLILIAVRVVHQLNKYSSIESMKNFKITNTKK
                     RILESVRHSNMTKVLSHKYMTQKKGLFVGILFSLSLGGIIFLCSSYSIQLTKNNNELT
                     MKADDGLNSDYQITMQTTDFDVGIPKDKISRLNNVEGISKVYPVRYLFGAIQIKEEQL
                     FWKNFFKPLEKDYRIKTFFDGICTKQNEGGYLLKTNIYGYNNGMLEGLNSYIIDGVIN
                     THDMVKNNKVIVRLPMDGTGMYDAVDINPGDTIKVKVPKTMKPTDEIVKFKEENKNDY
                     TTKEFVVAATVKRVMANNIYFIGDYGMDIVMTNGQMKNNFDIENYNSVSIKKTEDSNS
                     VGLSENIKSVVSDVKRCIVTDYTIAIEKNNTYLNQKLLFIYGIVFVLLAISLFHIINT
                     VSYLIFSRRHEFGILRAMGITDNKFSLMMIREGFLYGFYASIIMVIGSVIGQFMIYFM
                     VKRVYLYINPILKINAPLYIGMIISNITISIVAVIIPVRQILKSDIISEINKS"
     gene            complement(5208..6407)
                     /locus_tag="KRL67_002997"
     CDS             complement(5208..6407)
                     /locus_tag="KRL67_002997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003493023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="coenzyme F420-0:L-glutamate ligase"
                     /protein_id="HBH1523863.1"
                     /translation="MDRLVGTVSRGVRAPIIRQGDDLVKIVVDSVLNASKSENFEVRD
                     KDVIAVTEAVVARAQGNYAHVDNIAKDVKDKFGDDTVGVIFPILSRNRFAICLKGIAK
                     GCRKVVLMLSYPSDEVGNHLISIDELDDKGINPWSDVLTEEKYRELFGYNKHTFTGVD
                     YVDYYKNLIKDCGAEVEIVFANNPKTILNYTTSILTCDIHTRQRTKRILRQNGAKKVY
                     SLDDIMTASVDGSGYNDQYGLLGSNKATEETVKLFPINCDEVVNKIQGNIKEITGKDV
                     EVMVYGDGAFKDPVGKIWELADPVVSPAYTKGLEGTPNEVKLKYLADNDFADLSGDEL
                     KEAISKYIVEKDNKSDDLTGNMVSQGTTPRRLTDLIGSLADLTSGSGDKGTPIIYIQG
                     YFDNYTK"
     gene            6526..7413
                     /locus_tag="KRL67_002998"
     CDS             6526..7413
                     /locus_tag="KRL67_002998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBH1523864.1"
                     /translation="MNIKLELYKVFNAVVNNKSFSLAAKELFMSQPAVSQSIKQLEEQ
                     LDTLLFYRNNKGVKLTPEGKILSEHVTTALKLISSGEDRINKFKKLEYGSLKIGVGDT
                     AARFFLLKYLEIFHKKYPHIHVSTINRTSRELISLLKDGNIDIAIINMPIEDDTLNIV
                     ECIEIHDIFVCANDYIEYKGRKISLEELNTLPLIMLENKANSRLYVNEYFLSKGIKLN
                     PDIELGSHELLLEFAYINLGVSCVIEEFSIDYLENGKLFKLDIKEPIPKRNIGYCHLK
                     DISLSLATKEFLSMISNNI"
     gene            7603..8160
                     /locus_tag="KRL67_002999"
     CDS             7603..8160
                     /locus_tag="KRL67_002999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBH1523865.1"
                     /translation="MSDSEYPQMKTLETRNCILRPASLDDVEDFFDCYKNKIVVKHLP
                     FSEHKSLEDTRKFIKSFFLNPYKKGKIGHFAIVYKRDNKVIGNMGFNNINPKALEAEI
                     GICINPSYWGHDFATEITKRVIQYGFRELNLNKIIAITYEENQNSTKSLDLLGFKCTG
                     KYIKKIHTGNTVKNVPCYQYELKKQ"
     gene            complement(8401..9261)
                     /locus_tag="KRL67_003000"
     CDS             complement(8401..9261)
                     /locus_tag="KRL67_003000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HBH1523866.1"
                     /translation="MKKQKSPILLEMLGLFLGCISMSIGINMFLKPHTIAPGGLSGLS
                     LVLNKVTGLPVSAIMLIIGVPLVILAFRIMGTKNSLKTLFGTVVFSAIVQLTDPLSKL
                     RFTNDLLLSSIAGGILVGIGLGIMFKSDASTGGTDLIALILSKKFPGIKATKFMSCLD
                     GMVVISSGIVNRSLETALYSGIALCVLIKIADMIMEGFDHSKAFFIISDEPESLRQAI
                     TEELDRGLTILDGKGGYTRENKEVLLVVVSKKQELYLKRLVKRTDPTAFVIVTDVHEV
                     LGEGFKNLEN"
BASE COUNT         3707 a         1162 c         1408 g         3256 t
ORIGIN      
        1 ttattttgaa aacaaattta aatacaatct ttttagtgaa ataaataaaa ttaaaatatt
       61 tttagtatga taatgcaaga aaataattat ttatctaaaa aatctaaagg agagtaaagt
      121 tgaacttatt aattattgaa gatgatataa acttaaatga aggtcttttt tatgcatttg
      181 agaatgatgg atttaatgta tttaaggctt atacaaaaca agaaggatta aatatattta
      241 acagtaaaaa tatagatttt ataatcttag actgcaatct tccagatgga gatggatttg
      301 atgtatgtca actaataagg gaaaagtcag atataccaat tataatgctt actgcaagag
      361 actcagaaat tgatgaagta aaaggattag aaatagggct tgatgattat attacaaaac
      421 cattttcttt atctgtatta aaggcaagag tcaaagtagc aataagaaaa aaatcgaata
      481 ataaagtaat atattccaat ggaataaaat tggaccaaaa gctactaaaa gtatataaaa
      541 ataaagtatg tttggaactt agctctgtag aatataagct tctaagttat ctaattgaaa
      601 ataaaggtca aatattatta aaggaacaaa ttttacacca tatttgggac agcgaagaaa
      661 attatgttga tgagaatatt gtgtctgtaa atataagaag acttagagta aaagtagagg
      721 atgacccatc taatccaaaa tatataaaaa cagcttatgg aatgggttat ttatggaatg
      781 aggtagaata agatgtttat attagccttt ataatatgta cagttgttat atttatagta
      841 tctactaaaa taaatcaaaa aagatatgat gaattaagtt taatgttaaa tcaaatattg
      901 gaaggtaaag aagttactta tccagatacg aaggatacaa gagcgtcaaa aatttctcaa
      961 caagtaaaaa aaattaagga tatgatagaa atagaggtag aacagtcaaa gttagaaaaa
     1021 gaagcgatta agggtttaat atcaaatatg tcacatcaat taaaaactcc actttcaaat
     1081 ataaccattt attgtgaact tctggagaat ataaatattt ccacattaca gaagaaagag
     1141 tttttacaaa agatgaaaaa tgaaactttt aagattgact ggttgttaca atccctattt
     1201 aaaatgacca aattagagga tggagttata gaatttgaag tagaagaact actaattaaa
     1261 gatactctta ttcagagtat cagtactatc tttaataaag cagaagctaa aaatataagg
     1321 gtaaatcttg aaccattttc agatataaag ttagttcata ataaaaagtg gaccatagaa
     1381 gcaattgtaa atgttttaga aaatgcaata aaatactcac catcagatag cacgattact
     1441 atttctgtaa taaaaatgga actgtatacg aagattacta ttaaagatga aggaataggt
     1501 attgatagca gagaactaaa tgatattttt aaaagatttt acagaagtaa aaatgtggaa
     1561 aaccaaaatg gtacagggat tggcctatat cttacaagat taattttaga aaaagaaaat
     1621 ggaaacatta tagtagaatc taaactagga agtggatgtt gttttagcat cttcttacag
     1681 aattgtaaga gtttaaacta actttgtagg atgagtgtaa ttatcgtcac ttacaatatt
     1741 ttatagaaca tatatctatt gaaaggagaa attaaatgag tatattagtg acgaataatt
     1801 tagtaaaaca ctatggaaaa gatgagacta aagtaaaagc cttgaacgga gtaagtatag
     1861 aaattgaacg agctacattt actgctatag ttggaacttc tggttctgga aaaagtacat
     1921 tgttaaatat tataggtgga ttagatagtc caacctcagg tgatgtaata attaaaggaa
     1981 agaacatatc taaaatagga aaaaaagatt tgacagtatt tagaagaaga aatataggat
     2041 ttatatttca aaactacagt ttaatgccag tattaaacac ttatgataat atagctcttc
     2101 ctgttacatt agataaagga aaccatatag accatgaata tattgaaatg ctgatgaaga
     2161 cattgggaat ttgggataag cgtttaaaat ttccaagcga actatctggt ggtcaacaac
     2221 agagggtagc aattgctaga gctttagcaa ataaacctgc acttatatta gcagatgaac
     2281 caacaggaaa tcttgatagt aaaactacaa tggaagtggt atgtttatta aaagaaagta
     2341 gtgcaaaatt ccatcaaacg atacttatgg taacacacaa tgaaaatata gcacaagtat
     2401 gcgactcaat tatccatatt gaagatggag ttgtggtgaa tactggtggt gaagtactat
     2461 gatgtctcta gtaaaatttt caattcaata tataaagcat tataaaaaac aatctatatc
     2521 tatagttttg agcatagtac tatcagtagc attattgacg ggaattggtt ctttagttca
     2581 tagtgcagac aaaagtagaa ttgaaaagat aagggaagat agtggagatt atcattattt
     2641 ctataaagta gataaggaac aacttaataa aattaaagaa aataagaaat caaaggaata
     2701 cactgtagat agacttggtg ttacaaaacc taaaaatcag atagatgaac cttttattat
     2761 agaattttta aatgctgatt cttcatattt agacatgaca ggtagaaaat tattaaaagg
     2821 aactcttcct agtaaagatg gagagatagt acttgacaca tattctctaa acaatttaaa
     2881 tgtaaacaaa gaaattggaa cagaaatcaa tttagatggg aaaatctata agttatgtgg
     2941 tattttatca agtctttcaa acccagaaac cgctatggag ggatttgtaa acgattattt
     3001 acagcctaaa gaaaaggatt cttatatagt ttatgtaaaa tatgatgaaa ataaaaatat
     3061 taaaaagcaa aattataaat ttatgaaaaa attcaacata tcaaaagaaa aagcttatat
     3121 aaactggcct ctttcagttg aatttggagt aaaaccacca aaaaatatag aaaaatataa
     3181 tatatttgag ttcttaaaaa atttagaatt aaatacaaat gcaattatat tacttgttgg
     3241 agtgtttggt gcagcagcag tatatagtat tttccatgtt tcaatacttc agagaatatc
     3301 acaatatggg gttttagaag tattaggtgc taataataaa caacttttat ttctactatt
     3361 tttagaatta tttttacttt ttattgttgg atttcctata ggttgcttcc taggtattgg
     3421 agtagcgtct acaatatatg aacaatttcc acatatattt ttgagtagtg atattgttcc
     3481 aggagcattt tttataagtg aaaaatctat ttatttagga tttttattta tcttattgtt
     3541 gttaatttta attgctgtac gtgttgtaca tcagttgaat aaatactcta gtatagaatc
     3601 tatgaaaaac tttaagatta ctaatacgaa aaagagaata ttagaaagtg taagacattc
     3661 taacatgaca aaagtattat ctcataaata catgactcaa aaaaaaggat tgtttgttgg
     3721 aattttgttt tcattatcac ttggtggaat tatatttcta tgttctagtt actctataca
     3781 gcttactaaa aacaataatg aacttacaat gaaagctgat gatggtttaa attctgacta
     3841 ccaaataact atgcaaacaa cagattttga tgtaggtata ccaaaagata aaatttcaag
     3901 attaaacaat gttgagggaa tatcgaaagt ctatccagta agatatttat ttggagctat
     3961 acaaattaaa gaagaacaac tattttggaa aaatttcttt aaacctcttg aaaaagatta
     4021 caggataaag actttttttg atggtatttg tacaaagcaa aatgaaggtg gctatctttt
     4081 aaaaacaaat atttatggtt ataacaatgg tatgttagaa ggattaaatt cttatattat
     4141 agatggggtg attaataccc atgatatggt taaaaataat aaggttatag ttagactacc
     4201 tatggatgga actgggatgt atgatgcagt tgatataaat ccaggagata caataaaagt
     4261 taaagtgcca aagacaatga aaccaacaga tgaaattgtt aaatttaaag aagaaaataa
     4321 gaatgattat acaacaaaag aatttgtagt tgctgctact gtaaaacgtg tgatggcaaa
     4381 taacatatat ttcattggtg attatggtat ggatatagtt atgacgaatg gacaaatgaa
     4441 aaataacttt gatatcgaaa attataattc agtaagtata aaaaagacag aagatagtaa
     4501 tagtgtggga ttatctgaga acataaaaag tgttgtaagt gatgtaaaaa gatgtatcgt
     4561 aacagattat actattgcta ttgaaaaaaa taatacctac ttaaatcaaa aactcttatt
     4621 tatatatggt attgtatttg tacttcttgc aataagttta ttccatataa tcaatacagt
     4681 aagttacttg attttttcaa gaagacatga gtttggaatt ttaagagcta tgggtattac
     4741 agataataag ttttcactta tgatgataag agaagggttt ttatatggat tctatgcaag
     4801 tattataatg gtaattggaa gtgttattgg acaatttatg atttatttta tggtaaaacg
     4861 tgtatatcta tatataaatc caattcttaa aataaatgca cctctataca taggtatgat
     4921 tatttcaaat atcactatta gtatagttgc tgtaattatt ccagtaagac aaattttgaa
     4981 gagtgatatt atttcagaaa taaataagag ttagagttat ataaattttt aagattatat
     5041 aaatttctga caatcttgtt taaattattt aaatatatct tatctgtgaa cattaaataa
     5101 acctcttaat agatataact cttgaaaagt catatctatt aagaggtttt aataaatttt
     5161 catgctaata tatataatcc aaaaaattta aatcttatta aaacatacta ttttgtatag
     5221 ttatcgaaat atccttgtat gtaaataata ggggtaccct tatctccact tccagatgtt
     5281 aaatctgcta aagaacctat aaggtctgtt aatcttcttg gagtagtacc ttgagaaacc
     5341 atgtttcctg ttaaatcatc agatttatta tctttttcaa ctatgtattt tgaaattgct
     5401 tcttttaact catcaccaga taagtcagca aaatcattat ctgctaagta ttttaatttt
     5461 acttcgtttg gagttccttc taatcctttt gtatatgcag gagatactac tgggtcagct
     5521 aattcccata ttttacctac agggtcttta aaagctccat ctccataaac cattacttct
     5581 acatctttcc ctgtgatttc ttttatatta ccttggattt tgtttacaac ttcatcacaa
     5641 tttataggga ataattttac tgtttcttct gtagctttgt ttgaacctaa aagtccatac
     5701 tggtcattgt aaccactacc atctacactt gctgtcatta tatcatcaag tgaatatact
     5761 tttttagctc cattttgtct taaaattctc tttgttcttt gtcttgtatg tatatcacaa
     5821 gttaaaatac ttgttgtata gttaagaata gtttttggat tgtttgcaaa tactatttct
     5881 acctctgcac cacaatcctt gattaaattc ttataataat caacataatc tacaccagta
     5941 aatgtatgct tgttatatcc aaatagttct ctgtattttt cttctgttaa aacatctgac
     6001 caaggattta ttcccttatc atcaagttca tcaatactta ttaagtgatt tccaacttca
     6061 tcagaagggt agcttaacat caatacgact tttctacatc cttttgcaat tccttttaaa
     6121 catatagcaa aacgatttct acttaaaatt gggaatataa ccccaacagt atcatctcca
     6181 aatttatctt ttacatcttt tgcaatattg tcaacgtgag cataatttcc ctgtgctcta
     6241 gctacaacag cttctgtaac ggcaattaca tctttgtccc taacttcaaa gttttcactt
     6301 tttgacgcat ttaaaacaga atcaacaact attttaacta agtcatctcc ttgtcttata
     6361 ataggtgctc taacccctct tgaaacagtt ccaactaatc tatccattat ctaaaatcct
     6421 cctaaagaat taatattgtc atattagtac catgtatatt ataattcaaa tataattata
     6481 agtaaaataa ataatactaa tatttgacat aaggagtaat tatatatgaa tataaaactt
     6541 gaactttata aggtttttaa tgcagttgta aataataaga gtttttctct tgctgctaag
     6601 gaattgttta tgtcacagcc agctgtaagc cagtcaataa agcaattaga agaacaatta
     6661 gatactttac tattttatag aaacaataag ggagtaaaat taactccaga ggggaaaatt
     6721 ctaagtgaac atgtcactac tgcattaaag ttaatatcat caggagaaga tagaataaat
     6781 aaatttaaaa aattagaata cggttctctg aaaataggag taggagatac agctgcacgt
     6841 tttttcttgt taaaatattt agaaattttt cataagaagt atccacatat acatgtatct
     6901 acaataaata gaacgagtag agaacttata tctttactca aagatgggaa tatagatata
     6961 gcaattataa acatgccaat agaagatgat acactaaata ttgttgagtg tattgaaata
     7021 catgatatat ttgtatgtgc aaatgattac atagaataca aaggtaggaa aatatcatta
     7081 gaagaattaa atacattgcc tttaataatg ctagaaaata aagcaaattc aagactttat
     7141 gtaaatgaat attttttatc aaaaggtata aaactaaacc cggatataga attgggttct
     7201 catgaattgc ttttagaatt tgcgtatata aatttaggag tgtcttgtgt tatagaagaa
     7261 tttagcatag attatttgga aaatgggaaa ctgtttaaat tagatataaa agagccaata
     7321 cctaagagaa atataggtta ctgccattta aaagatatat ctttatcctt agcaaccaaa
     7381 gaatttttaa gcatgatatc taataacata taatttagaa tacatatttc acaagtaatt
     7441 taatataaaa atagagataa gttcgtaaat ttgaaagatt catatagtaa ttagttttta
     7501 ttaaaaataa aaataaggtt aatttgagaa catttatata attaatatcg tattttaaat
     7561 atgaacagta tatttagatt aatgtataaa gggggattag aaatgtctga ttctgaatat
     7621 cctcaaatga aaaccttaga aactagaaat tgtatactaa gacctgcatc attagatgat
     7681 gtcgaagatt tttttgactg ttataaaaat aaaatagtag taaaacattt accatttagt
     7741 gagcataaat ctttagaaga tacaagaaag tttataaaat cattttttct aaatccctat
     7801 aaaaaaggga aaattggaca ctttgctata gtatataaaa gagacaataa agttattggg
     7861 aatatggggt ttaataatat aaatcctaaa gcattagaag cagaaatagg tatatgtata
     7921 aatccaagct attgggggca tgattttgct actgagataa caaaaagagt aatacaatat
     7981 ggatttagag aactaaactt aaataaaata attgcaataa cctatgaaga aaaccaaaat
     8041 tctacaaaat cattagattt attaggattt aaatgtacag gaaagtatat caaaaaaatt
     8101 catactggaa atactgtaaa aaatgttcct tgttatcaat atgaattgaa aaaacaatag
     8161 atataatcat ttaatattta tgctagttat ttaataatga ttgttttcta aataaaattt
     8221 caataaagtg gttgcctcaa aatatagata agcctgtttt ttattgttaa aatactgaaa
     8281 atgcatttga ttttatacaa aaaagagtgt ctcatgaact aaaaagttta tgagacactc
     8341 tttttgaata aatcgtattt taattaaaat agtatatata cttaaaatat taatttttta
     8401 ttagttctct aaatttttaa atccttctcc aagaacctca tgcacatctg taacaataac
     8461 aaatgcagta gggtcagttc ttttaactaa tctctttaga taaagttctt gttttttaga
     8521 aactacgact aatagaactt ctttattttc tctagtatat ccacctttac catcaagaat
     8581 agtaagacct ctatcaagtt cttcagttat agcttgtctt aaactttcag gctcatcaga
     8641 aataataaaa aatgcttttg aatggtcaaa tccttccatt atcatatctg ctatttttat
     8701 taatacacaa agtgctatac cagagtaaag tgcagtttct aaactacgat ttactatacc
     8761 agaagatata acaaccattc catctaggca tgacatgaat tttgtagctt ttattcctgg
     8821 gaatttctta ctaagtatga gtgcaattaa gtctgttcct ccagtagaag catctgattt
     8881 aaacattatt cctaaaccta taccaactag gattcctcct gcaatagaag acaataataa
     8941 gtcattagta aatcttagct ttgataatgg gtctgttaat tggactatag ctgaaaatac
     9001 aactgttcca aataaagttt ttaatgaatt ttttgtaccc ataattctaa aagcaagtat
     9061 tactaatggg acacctataa ttaacatgat agctgatact ggaagtcctg taaccttgtt
     9121 taatactagt gatagaccac taagtcctcc aggagcaatt gtatgtggtt ttaaaaacat
     9181 atttatacca atactcattg atatacaacc caaaaatagt ccaagcattt caagtaaaat
     9241 tggcgacttt tgttttttca tataaacacc tccatgaact tgtcacaatt taatattata
     9301 acatatttga ccagtttata ttatataaaa tttcttgata taagcaattt caagtatttc
     9361 attattctta atatttttga atttttatat agtgttttca tatttttact gatgttgtta
     9421 cactcctatg aagtttttta tatattataa ttttttaatt cttttataaa gtatccccta
     9481 taaagatggt tctatgaaaa atttctctat actatttaac gcatataaaa att
//
LOCUS       DAEPXF010000152         9423 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain HB27
            SAMN13376389-rid12479423.denovo.159, whole genome shotgun sequence.
ACCESSION   DAEPXF010000152 DAEPXF010000000
VERSION     DAEPXF010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13376389
            Sequence Read Archive: SRR10518432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 9423)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9423)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001069624.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001069624.1
            Genome Coverage       :: 315x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/15/2021 01:54:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,165
            CDSs (total)                      :: 4,076
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 89
            rRNAs                             :: 10, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 10 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 19 of 69
            Pseudo Genes (incomplete)         :: 44 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 10 of 69
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9423
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13376389-rid12479423.denovo.159"
                     /strain="HB27"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="China: Hebei"
                     /collection_date="2017"
     gene            <1..415
                     /locus_tag="KRL67_003001"
     CDS             <1..415
                     /locus_tag="KRL67_003001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH1523867.1"
                     /translation="NGFKKTKPCNKIYKIIIVLILTCILFKSFMYVKSINELEDYISQ
                     NKLVDKSKHTLPTTDKTREKFSKIKLNEIKKIYETIGINSITKISIDKNKLEMEGVCS
                     DLDVLNNLRKIKYIKYYYIDSIVRDGEYYRFRIKS"
     gene            430..1014
                     /locus_tag="KRL67_003002"
     CDS             430..1014
                     /locus_tag="KRL67_003002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH1523868.1"
                     /translation="MRLNNREKNLLKILLILVIILASDIAVSYLEENLISLKNEKQNI
                     INKINNQDNYQIKDNKRIDDKYIESVELNKNEELKNNFNNSFLDKKTNKDIVLLVENE
                     LRDIVNVEYINQESSLDNHNKKQVNLEIKVNGELANVLKLEGVMKKLRLNNKLEKIEI
                     NKLKVNNFDSQNIPNEYKQEKDNIVECKMTVRVI"
     gene            1267..2304
                     /locus_tag="KRL67_003003"
     CDS             1267..2304
                     /locus_tag="KRL67_003003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SEC-C domain-containing protein"
                     /protein_id="HBH1523869.1"
                     /translation="MLGRNELCPCGSGKKYKKCCLNKDIVSERACRKTELSQKQYTDL
                     YSKLYDYSRQDKFKEECEKAKEMFYIMQNDSVNEKFERFFNTYLIQDHIMENKKVMTV
                     GFLEENGANLSQSEVSILKSMFESYVSIYEVKEVSAGKIVLKDCLSGNELCTEDVKLL
                     RSFKIGSCMIARIVDIEGTNILIDITISISNEVKDIILKDIMNLFNQYQDVYKDMKSF
                     LIYHTHILYKYIQQLLDPSIAEYLKRERDKNDTKVNSENELAEEECKVLDTLKENVEK
                     ENIDACIEFWNEYKKNHENIKGAENGWAAAVEYYVKKEAGQPITQVQISKKYDISSST
                     LGKRYKDLKIS"
     gene            2385..2750
                     /locus_tag="KRL67_003004"
     CDS             2385..2750
                     /locus_tag="KRL67_003004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH1523870.1"
                     /translation="MSLSLGNAIAVSTNFIKSNPAVPVYNGEEIYAVIYHIEIGYNNG
                     VSPDTKQYIIIDYKEFDLSMDKKNELINIAKEYCKSKGIVENENLEINLLDNEEAENF
                     LEKVFNNMKVIRGLITKKG"
     gene            2755..3072
                     /locus_tag="KRL67_003005"
     CDS             2755..3072
                     /locus_tag="KRL67_003005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH1523871.1"
                     /translation="MDYIGIENITPYENTYEFSVYEYDDEIILGSEKLYVCELRVVLI
                     KVNSLYVERLNKSVEAMVLVKNLKKDLDKTLVVNKIKNFVLDEIWVENLVKENIEVIF
                     VES"
     gene            3120..4724
                     /locus_tag="KRL67_003006"
     CDS             3120..4724
                     /locus_tag="KRL67_003006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH1523872.1"
                     /translation="MLRVSNVKLGIDKDISLLKDVVLKKLKIKEKNLVKYSIYKESID
                     ARKKGKMEFVYSVDVEVKDENKILNSKIKDVTKIKEIRYVNVPIGDKKLKNNPLVIGS
                     GPAGLFAGLLLAQMGYEPIILERGLDVDSRTKDISDFWTTRKLKNNSNVQFGEGGAGT
                     FSDGKLTTRIKDIRCRKVLEELVQFGAPDEILYSHKPHVGTDILKNVVKNIRNEIISL
                     GGQVRFNSKVTDILVSNDSIQSVIINDTEKIDTDTIILAVGHSARDTYEMLYSRGIKI
                     VQKPFAIGARVEHPQDLINKAQYKDFYNHPRLGAADYRLIEHVSNGRTAYTFCMCPGG
                     SVIASASNDFEIVTNGMSEHARDKINANSAFLVNVTPEDFKSDNPLAGVHFQQKYEKL
                     AFELGGKNYNAPVQLVGDFINNKTSLNLGSVEPSYRPGYTLTNLSECLPSFVTETMRE
                     AFISLDKKLNGFAMYDAVLTGVETRSSSPIRIVRDENTLESVSIKNLYPCGEGAGYAG
                     GIVTAAVDGIKCAEKIIQKYTNNTKI"
     gene            4848..6476
                     /locus_tag="KRL67_003007"
     CDS             4848..6476
                     /locus_tag="KRL67_003007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HBH1523873.1"
                     /translation="MNIIISLMGGLGLFLYGMNLMGEGLQKSAGTKLKKIIKLLTSNL
                     FMGVLVGTGVTAVIQSSSATTVMVVGFVNAGIMTLKQAIGVIMGANIGTTVTAQLVSF
                     DLTGMAPVALGVGIILYLFGNKPRIKNIAEILIGFGILFTGMDFMKMAVEPLRDYQGF
                     TDLLVTFGRYPLLGLLLGFGITAIIQSSSASMGMLVALAAEGLVPLSAALPILYGQNI
                     GTCVTSLLSSIGANKNARRAAMMHLIFNVLGTVIFLIFLNKPVVSMVTSWDPSNVARQ
                     IANTHTLFNIISVLILLPFTNLIIKLAIKLVPDRAGDIDEDETKTIKYIDDRMIETPS
                     IALANTIKEALRMGEKAKESLNASMEALVEHSTEKIDKTYRRERLINDLQKAILNYLL
                     KLSKAPLDDDSREVVDTLFNTVNDIERIGDHAKNIAELSQVAIDSNISFSEEGQSELD
                     VMYNRVVSAYTYALESMRTDNVDLACKVIKIEEQVDIMEKSCRANHMYRLNNNLCSIE
                     NGVIYLDVISNLERISDHAVNIAQQVIAKRLGND"
     gene            6741..8090
                     /locus_tag="KRL67_003008"
     CDS             6741..8090
                     /locus_tag="KRL67_003008"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HBH1523874.1"
                     /translation="MGKINFDYSKATEFFCQNEIDVMQPYVDVAHDMLHNKTGLGNTF
                     LGWIDLPKNYDKEEFDRIKKSAEKIKSDSDVLLVIGIGGSYLGSRAAIDMVSHSFRNG
                     LKKEQRKAPEVYFVGHNISSTYIMDLLDIIEGKDISVNVISKSGTTTEPALAFRIFKD
                     YLEKKYGKEEARKRIYATTDSSKGALRQLATEEGYETFVIPDDVGGRFSVLTAVGLLP
                     IAAAGLDIDAMMKGANDAREAFQNPDLKSNDSYRYAVARTILHRKGKDVELLVNYEPQ
                     LHYISEWWKQLYGESEGKENKGLFPASVDFSTDLHSMGQYIQDGKRLLFETVLNVENC
                     KRNITISSEEVDLDGLNYLAGKTVDFVNHKAFEGTLLAHTDGKVPNLVINIPQLDEYN
                     FGYLVYFFEKACGISGYLLGVNPFDQPGVEAYKKNMFALLGKPGYEKEKEELEKRLK"
     gene            8246..9325
                     /locus_tag="KRL67_003009"
     CDS             8246..9325
                     /locus_tag="KRL67_003009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HBH1523875.1"
                     /translation="MSRRKKGISLVILVAIISSILSSFLTIILVKDNLVSKSTGSSTP
                     IVVNDDGKSQNIYQAVAEKATPSVVGITTTSVDTSNMFAIPTETQGVGTGIIVDSNGY
                     ILTNSHVISDGQATSVNVLFNDGSTTSGKVVWFDQQLDLAIVKVDKTGLTPAEFADSD
                     KVKVGDISIAIGNPLGLDFQKTVTQGIISGLDRTIQTEKTNMTGLLQTDASINAGNSG
                     GPLLNQKGQVIGINTAKASQAEGLGFAIPINTAKSIVEEVIKTGKYEKVTLGIKGTDV
                     SNYEAATGTKLSTDKGVYVAEVISGSSAEKAGVKVGDIITKVGDTDITGMNDLNKKLY
                     TFSKGASTKITVNRGGKAVTINVNF"
BASE COUNT         3892 a          954 c         1697 g         2880 t
ORIGIN      
        1 taatggattt aaaaaaacta aaccatgtaa taaaatatat aaaataataa tagtcttaat
       61 tttaacgtgt attttattta aatcatttat gtatgtgaaa tctataaatg aacttgaaga
      121 ttatataagt caaaataagc tagtggataa atcaaagcac actttaccaa caacagataa
      181 aactagagag aaattttcta aaataaaact taatgaaata aaaaaaatat atgaaacaat
      241 tggaataaat agtattacta aaatatcgat agataaaaat aagctagaga tggaaggtgt
      301 gtgtagtgat ttagatgttt taaacaatct aaggaaaata aagtacataa aatattatta
      361 catagacagt attgtaagag atggagaata ttatagattt agaataaaaa gttagatggg
      421 tgattatttt tgagattaaa taatagagaa aaaaatctac ttaaaatatt gttaatatta
      481 gttattatat tggcaagtga tatagcggta agttatttgg aagaaaacct aattagtttg
      541 aagaatgaaa agcaaaatat aataaataaa ataaataatc aagataatta tcaaataaag
      601 gataataagc gtatagatga taaatatata gaaagtgttg aattaaataa aaatgaagaa
      661 ttgaaaaata attttaataa ctcattttta gataaaaaaa caaataaaga tatagtgtta
      721 ttagtagaaa atgaactgag agatattgtg aatgtagaat atattaatca agaaagtagt
      781 ttagataatc acaataaaaa acaagttaat ttagaaataa aagttaatgg agaacttgca
      841 aatgtgttga aacttgaagg tgtaatgaag aagttaagac taaataataa gcttgaaaag
      901 atagaaatta acaaattaaa agtcaataat tttgattctc aaaacattcc aaatgaatat
      961 aaacaagaaa aagacaatat tgttgaatgt aaaatgacag ttagagtcat ataacaaaaa
     1021 agaatagata aagtaaaaaa ctattatcaa ttaataaaat tttatagagg aattagtttt
     1081 acgataattc atttctacaa gagggattgc tgatgatttt atcgaaatcc taattaaagt
     1141 taatatattg taccaaaata taagtgaatt taatagaata tttacttata ctatagattg
     1201 attaataaaa aaatggtata ataacaaatt gtactttaat ttaataataa gggagtggaa
     1261 gtcatattgt tagggagaaa tgagctatgc ccttgtggaa gtggcaaaaa atacaagaaa
     1321 tgttgtttaa ataaagatat tgtttcagaa agagcatgca gaaaaactga actttcacaa
     1381 aaacaatata cagatttata ttcaaagtta tatgattatt caagacagga taagtttaaa
     1441 gaagaatgtg aaaaagctaa agaaatgttt tatataatgc aaaatgatag tgtaaatgaa
     1501 aaatttgaaa gattttttaa tacttattta atacaagacc acataatgga aaataaaaag
     1561 gttatgacag tcggttttct tgaagaaaat ggtgcaaacc taagtcaatc agaagtatcc
     1621 atattaaaaa gtatgtttga atcatacgta agtatttatg aggtaaaaga agtttctgct
     1681 ggaaaaatag tattaaagga ctgtttaagt ggaaatgaac tttgtacaga agatgtaaaa
     1741 cttctaagaa gttttaaaat cggaagttgt atgatagcta ggatagttga tattgaagga
     1801 actaacatat tgatagatat aactataagt atatcaaatg aagttaaaga tataatttta
     1861 aaggatatta tgaatctatt caatcaatat caagatgtat acaaagatat gaagtcgttc
     1921 ttaatttatc atacacatat actatacaag tatatacaac agcttttaga cccaagtata
     1981 gctgaatact taaagagaga aagagataaa aacgatacaa aagttaattc agaaaatgaa
     2041 ttagctgaag aagaatgtaa agttttagat actcttaaag aaaatgtaga aaaagaaaat
     2101 atagatgcat gtatagagtt ttggaatgaa tataagaaaa atcatgaaaa tataaaaggc
     2161 gctgaaaatg gttgggcagc tgctgttgaa tactatgtta aaaaagaagc aggacaacct
     2221 ataactcaag ttcaaatatc aaagaaatac gatataagct ctagtacact aggaaagaga
     2281 tacaaagatt taaaaatatc ttaattaatt ataaaaagct ttaagtcata gataaagaat
     2341 ctatagtata atatatgtat aatgtttaga aagaggtatt aataatgagt cttagtttag
     2401 gaaatgcaat tgctgtatca acgaacttta taaaatctaa cccagctgta cctgtgtaca
     2461 acggagaaga aatatatgca gttatttatc atatagaaat aggatataat aatggtgtat
     2521 cacctgatac caaacaatat ataataatag actataaaga gtttgattta tctatggata
     2581 aaaaaaatga actaataaat attgctaaag agtactgtaa atctaaaggt attgtagaaa
     2641 acgaaaacct tgaaataaac cttttagata atgaagaagc agagaacttc ttagaaaagg
     2701 tgtttaataa catgaaggtt ataagaggtc ttataactaa aaaaggataa ataaatggac
     2761 tatataggaa tagaaaatat aacaccttat gaaaatacat atgaatttag tgtatatgaa
     2821 tatgatgatg aaatcatctt aggtagtgaa aagttatatg tgtgtgaatt aagggttgta
     2881 ttgattaaag ttaattctct gtatgttgaa agattgaata aatcagttga agcaatggtc
     2941 ttagtaaaaa acttgaaaaa agatttagat aaaacacttg ttgtaaacaa aataaagaat
     3001 tttgtgctag atgagatttg ggtagaaaat ctagtaaaag agaatataga agttatattt
     3061 gtagaaagct aggaataacc ctagcttttt tatattacaa tctaagagag gaaaacaata
     3121 tgttaagagt atctaatgta aaacttggaa ttgataaaga tatatcttta ttaaaagatg
     3181 ttgtacttaa gaagctaaaa ataaaagaaa aaaacttagt caaatatagt atatataaag
     3241 aatctataga tgctagaaaa aaaggaaaaa tggaatttgt ttattctgta gatgtagaag
     3301 ttaaagatga aaataaaata cttaactcaa aaataaaaga tgtaacaaaa ataaaggaaa
     3361 tcagatatgt aaatgtaccc ataggtgata aaaaattgaa gaataatcct ttggtaattg
     3421 gtagtggacc agctggtttg tttgcaggtc tgcttttagc tcaaatgggt tatgaaccaa
     3481 taatacttga aagaggtctt gatgtagaca gtagaaccaa agatataagt gatttttgga
     3541 caactagaaa attaaaaaat aattcaaacg ttcaatttgg agaaggtgga gcaggaactt
     3601 tttcggatgg gaaacttaca actagaataa aagatatacg atgtagaaaa gtattagaag
     3661 agttagttca gtttggagca ccagatgaaa tattatactc acataagcca catgttggaa
     3721 cagatatatt aaaaaatgta gttaaaaata taagaaatga aataataagt ttaggtggtc
     3781 aagttagatt taattcaaaa gtaacagata tacttgtatc aaatgattct atacaatctg
     3841 taataataaa tgatactgaa aaaatagata cagatacaat tatattagct gttggacata
     3901 gtgcaagaga tacttatgaa atgttgtatt caagaggtat aaaaatagtt caaaaacctt
     3961 ttgcaatagg agctagagtt gaacatccac aagatttaat aaataaagca cagtacaagg
     4021 atttttataa tcatcctagg ttaggagctg cagattatag acttatagag catgtttcaa
     4081 atggaagaac agcatatact ttttgcatgt gtccaggagg tagtgtcata gcatctgcat
     4141 caaatgattt tgaaatagtt acaaacggta tgagtgaaca tgcaagagat aaaataaatg
     4201 caaatagtgc atttttagta aatgtaacgc cagaagattt taagagtgat aatccactag
     4261 caggtgtaca tttccagcag aagtacgaaa aattagcttt tgaattaggt ggaaaaaatt
     4321 ataatgcacc agttcaactt gttggagatt ttataaacaa taagacttct ttaaatttag
     4381 gaagtgttga gccatcatat agaccaggat atacacttac aaatttaagt gaatgtttgc
     4441 ctagctttgt aacagagact atgagagaag catttatatc tcttgataaa aaattgaatg
     4501 gatttgcaat gtatgatgct gttttgactg gagtagaaac taggtcatca tcaccaataa
     4561 gaatagtaag agatgaaaat actttagaat cagtgagtat taagaatcta tatccatgtg
     4621 gtgaaggtgc tggatatgca ggtggtatag ttacagcagc agttgatggt ataaaatgtg
     4681 cagaaaaaat aatacaaaaa tacaccaata atacaaaaat ttaacataaa cttaatgatt
     4741 ttttaatatt taacatgaat ttaacattat cactcctata atttgataaa atacaatagg
     4801 ttataaaatt tttagataca gtaatctctt tacaaggagg aaataatttg aacattataa
     4861 taagtctaat gggtggatta ggtctgttct tatatggtat gaaccttatg ggagaaggtc
     4921 ttcaaaaatc tgctgggaca aagctaaaaa agataataaa actacttact agtaacctat
     4981 ttatgggagt tttggtaggt acaggagtta cagcagtaat acaaagttca agtgctacta
     5041 ctgtaatggt tgttggtttc gtaaatgcag gaataatgac tttaaaacag gcaataggag
     5101 ttataatggg tgctaatata gggactactg ttacagccca attggtatct ttcgacttaa
     5161 ctggtatggc cccagtagca ttaggtgtag gtattatact ttatttattt ggaaataagc
     5221 ctagaataaa aaatatagcc gaaatactta taggttttgg tatattattt acaggtatgg
     5281 attttatgaa gatggcagtg gagccactaa gagactatca aggatttact gacttacttg
     5341 ttacatttgg tagatatcca ctacttggat tattattggg atttgggatt acagcaataa
     5401 tacaaagttc aagtgcatct atgggtatgt tggttgcact ggcagcagaa ggtctagtgc
     5461 cattatcagc agcactgcca attctttatg gccaaaatat tggtacatgt gtaacttcac
     5521 tgttatcaag tattggtgct aacaaaaatg cacgaagagc agctatgatg catctaatct
     5581 tcaatgtttt aggaacggtg atattcttaa tattcttgaa taaaccagta gtaagcatgg
     5641 ttacaagttg ggacccaagc aatgttgcaa gacaaatagc caatacacat acattgttta
     5701 atataatatc tgtattgata cttttaccat ttacaaatct tattataaaa ctagctataa
     5761 agcttgttcc tgatagagca ggtgatatag atgaagatga aactaaaact attaaatata
     5821 tagatgatag aatgatagaa actccttcta tagctcttgc aaatacgata aaagaagcac
     5881 ttcgtatggg tgagaaggct aaggaaagcc taaatgcatc tatggaagct ttagtagaac
     5941 attcaactga aaaaatagat aagacatata gacgagagag attaatcaat gatttacaga
     6001 aagctatttt aaattactta cttaaacttt caaaagcacc tttagatgat gattcaagag
     6061 aagttgtgga tactttgttt aatacagtca atgatataga aagaattggt gaccacgcta
     6121 aaaatatagc tgaattatcg caagttgcaa tagattcaaa tatttctttc tcagaagaag
     6181 gacaatctga acttgatgtt atgtataata gagttgtttc agcatatact tatgcattag
     6241 aatcaatgag aactgataat gttgatttag cttgcaaggt tataaaaatt gaagaacaag
     6301 ttgatataat ggagaaatct tgtagagcta accatatgta tagattgaac aacaatctat
     6361 gtagtataga aaatggagtt atttatttag acgtaataag taatttagag agaatatctg
     6421 accatgctgt caatatagca caacaagtta tagcgaagag attaggaaat gattaaaaat
     6481 aattaattta aaataatata aaaattaaaa tagaggataa atacaagaga aactttaaat
     6541 gatacattta gagtttcttt ttttgtaaat ttatttgact aaaatttaaa aatttataca
     6601 tagttatagt atttagaaga atcgatttcc tggtaataaa tgatgagata tgtatgattt
     6661 tgtggcaaac ttttaaaaaa aacacctata ttttgtgtta taatctcttt aaaacgaata
     6721 tgctgaaaag agggattaaa atgggaaaaa taaattttga ttatagtaaa gctacagagt
     6781 ttttttgtca aaacgaaata gatgtcatgc aaccatatgt agatgttgca catgacatgt
     6841 tacataataa aactggttta ggaaatactt ttttaggatg gatagacctt cctaaaaatt
     6901 atgataaaga agagtttgac agaataaaaa aatctgctga gaagataaaa tcagactcag
     6961 atgttctttt agtaataggt ataggaggct cttatttagg ttcaagagct gctatagata
     7021 tggtaagtca ttcttttaga aatggactta aaaaagagca aagaaaggct ccagaagtat
     7081 attttgtagg tcataatata agctcaacat atattatgga tttattagat ataattgaag
     7141 gaaaggatat ttctgtaaat gtaatatcta aatcaggaac tactacagaa ccagctctgg
     7201 cttttagaat atttaaagat tacttagaga aaaagtatgg taaggaagaa gcaagaaaga
     7261 gaatttatgc aacaacagat tcaagtaaag gtgcacttag acagttagct acagaagaag
     7321 gatatgagac ttttgtaata ccagatgatg taggtggtcg tttttctgta ttgacagcag
     7381 ttggattact tcctattgct gctgctggac ttgatattga tgctatgatg aaaggtgcaa
     7441 atgatgctag agaagcattt caaaatcctg atttaaaaag taatgatagt tatagatatg
     7501 ctgttgctag aacaatttta catagaaaag gaaaagatgt agagttattg gttaactatg
     7561 aacctcaact acattatata agtgagtggt ggaaacagct ttatggagaa agtgaaggaa
     7621 aagaaaataa aggtttattc cctgcttctg tagatttctc aacagattta cattctatgg
     7681 gtcaatacat acaagatgga aaaagacttt tatttgagac agtacttaat gttgaaaact
     7741 gtaagagaaa tataactata agttcagagg aagtagactt agatggttta aattatttag
     7801 caggtaagac tgtagatttt gtaaatcata aagcttttga aggaactcta ttggctcata
     7861 cagatggaaa agtacctaat ttagtaataa atatacctca actagacgag tataattttg
     7921 gatatttagt gtatttcttt gaaaaggctt gtggaataag tggatattta ttgggagtta
     7981 atccatttga ccaaccagga gtagaagctt acaaaaaaaa tatgtttgct ttactaggta
     8041 aaccaggtta tgaaaaagaa aaggaagagc tagaaaaaag acttaaataa ttttaaatag
     8101 ttaaggttta attaagatta aaataatata atgaaatcat caaaaaggag tttactcaaa
     8161 atagatgatt tcattttttt tatgaaaaat tgggaaaaat atattttgat aatttatatt
     8221 atattaagat agggagtggt atgagatgtc aagaagaaag aaagggataa gtttagtaat
     8281 attagttgct ataataagtt caatacttag tagttttcta acaataattt tagttaagga
     8341 taatttagta agcaaatcta caggaagttc tacgccgata gttgtaaatg atgatggtaa
     8401 gagtcaaaat atttatcaag cagttgcaga aaaagctacg ccatctgttg taggtattac
     8461 aacaactagt gttgatacta gtaatatgtt tgcaatacca actgaaacac aaggtgtagg
     8521 aactggaatc atagttgatt caaatggata tatacttact aattctcatg ttatatcaga
     8581 tggtcaagct acaagtgtaa atgtattatt caatgatggt tcaacaactt caggtaaagt
     8641 agtatggttt gaccaacaac ttgatttagc tatagttaag gttgataaaa ctggactaac
     8701 accagcagaa tttgctgata gtgacaaagt aaaagttgga gatatatcaa tagcaatagg
     8761 taatcctctt ggattagatt ttcaaaaaac agttactcaa gggataataa gtggattaga
     8821 tagaactata caaacagaaa aaactaatat gactggttta ttacaaactg atgcaagtat
     8881 aaatgcagga aatagtggag gaccattgtt aaatcaaaaa ggtcaagtaa ttggtataaa
     8941 cacagcaaaa gcttctcaag cagaaggatt aggatttgca ataccaatca atacagctaa
     9001 gagtatagtt gaagaagtaa tcaaaactgg aaaatatgaa aaagtaactt tgggtataaa
     9061 aggtacagat gtatcaaatt atgaagcagc aacaggtaca aaattaagta ctgataaagg
     9121 tgtttatgtt gcagaagtaa tatctggttc atcagcagaa aaagctggag taaaggtagg
     9181 agatattata actaaagtag gagacacaga tataacaggt atgaatgatt taaataaaaa
     9241 gttgtatact ttctcaaaag gtgctagtac aaaaataaca gtaaatagag gcggaaaagc
     9301 agtaacaata aatgtgaatt tctaaatatg accagaaaga tggagactac atggtaaagt
     9361 gtagtctcta tttttattta atttttatag tatatgcatc taaattaatt aaaatgatgt
     9421 att
//
LOCUS       DAEPXF010000153         9410 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain HB27
            SAMN13376389-rid12479423.denovo.160, whole genome shotgun sequence.
ACCESSION   DAEPXF010000153 DAEPXF010000000
VERSION     DAEPXF010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13376389
            Sequence Read Archive: SRR10518432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 9410)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9410)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001069624.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001069624.1
            Genome Coverage       :: 315x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/15/2021 01:54:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,165
            CDSs (total)                      :: 4,076
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 89
            rRNAs                             :: 10, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 10 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 19 of 69
            Pseudo Genes (incomplete)         :: 44 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 10 of 69
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9410
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13376389-rid12479423.denovo.160"
                     /strain="HB27"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="China: Hebei"
                     /collection_date="2017"
     gene            114..1181
                     /locus_tag="KRL67_003010"
     CDS             114..1181
                     /locus_tag="KRL67_003010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide epimerase"
                     /protein_id="HBH1523876.1"
                     /translation="MKITDIKFEKLRIKLKKPVVVSFGVIEYGESIILKIETDEGYCG
                     FGEAAPLAAVTGEVLDNVLSVLLMFKKELIGKDPLDIETIHAIMDGIIIGNTSAKAAI
                     DIALYDIKGKIMNAPLYKVLGGFDNKVQTDITISIDKPEKMAVEALERVKEGFRILKL
                     KAGINPEDDIEAVKLIRKAVGESIRIRIDANQGWNVNSSINTIKKLEEFNVDAIEQAL
                     PHWDLDGTAYIRNKSNTKIMIDESLHSPIDAIKAIKKNAVDTFNIKLMKSSGIYPAIK
                     INNIAEASGVNCMLGCMLETRIGITAAANLIASKKNITEADLDSFMFCEELEGISGGF
                     VMDGDIMNLVNKPGLGIEVNL"
     gene            1200..2360
                     /locus_tag="KRL67_003011"
     CDS             1200..2360
                     /locus_tag="KRL67_003011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF819 family protein"
                     /protein_id="HBH1523877.1"
                     /translation="MNTLFSNPGSILAVMTSMIALGFYLQRYKAIKSLGPALTIIIMG
                     IILSNLKVVPVSTELYGTISTYAIPVSMTIMLMSVDLKEMTKLSREPLIAIFVAVLTV
                     SIMAFLFGLVFAEKISEGWKVAGMFVGTYTGGSANLTAIGTGLNVSRQTLAAANAADY
                     VIGVPTLIFMFALPAILKNSKKFKKLWPYHVEESELEDCQNEEFMESKEWSIKDIAWM
                     LAIGFVVTEVATILAGYFNSSFSSAARILLVTTISIIIAQLKPVKKLKGNLDLGLFVA
                     LFFLCTIGFSVDIKEFLGSTFTITLYCFSIIFASFIFHLGITRLLKIKYQYVILSIVG
                     AIADGPTSALVAASAKWNSLVSVAVVMGVIGGVLGNYAGISVAYAIKMSLGL"
     gene            2374..4470
                     /locus_tag="KRL67_003012"
     CDS             2374..4470
                     /locus_tag="KRL67_003012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CocE/NonD family hydrolase"
                     /protein_id="HBH1523878.1"
                     /translation="MIKFKIYISGLYSGNVIFGDDLLIEKLNPFTNKIESLKPISREE
                     NTYYLNLTKIDLKSLFKNFDVYTKSLVNTDKDTVINNLGETHSKLNEYIWVQRNKKFP
                     LDIIIVDNKIVGFICLSRETCTILIMDGYEEYTVLKEWDKTHKDEEIYSIRFGGNYMV
                     DMKDGIKLSTDVYLPNFIDSTKKAPTILMRTPYGKENDKEIYYKYVQRGYAVVIQDVR
                     GRNESEGKWEPMIHEREDGDSTINWIVSQEWSDGTVGMLGASYLGYVQWAAASSGNKH
                     LKALVSIVTSGSPFIDIPRKGGAFVSGMLAWAFMVSRNKVDRSKMVRDDWDDVLNIRP
                     IENIPVEALGYRIEFLEEWLKRVEKDEYWDLMDWHLQKDKINVPALVVSGWYDDNGMG
                     TTEALDVIKDYEKGKRKAILGPWMHNSNTLRDINGISLGNSSLRYDLDYNYLLWFDKY
                     LKGIENNIDTTAPVEYYSVGSNKWKTEENWPIINKIDRSMYLISDGNANTSLGNGRLV
                     FDSDLEEKYDSYIYNPKDPSVQLIDMSENEVGVPNSYKDLEKRSDMLCYTSDAFSEEF
                     TVTGDIKLEFFASSSAKDTDWVIKIMDVDLDGNSIKLADGILSARFRNSFYKSEFMEE
                     GEIYKFTIITSKISNTFKVGHKIRLDITSSAKNFIFQNSNTTEGYNSIEYIEAKNTIY
                     HGGKYPSKLVLPIENK"
     gene            complement(4629..4973)
                     /locus_tag="KRL67_003013"
     CDS             complement(4629..4973)
                     /locus_tag="KRL67_003013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HBH1523879.1"
                     /translation="MEIKRYEGTGRMSRAVVHNNTVYLCGQTHAEGGVIEQTTEVLAK
                     IEDLLNKYGSDKQHLLSVTIYLRDMKDFEAMNSVWDAWVEKGFEPARACVEARLAREH
                     LLVEMSVVAATK"
     gene            5265..6155
                     /locus_tag="KRL67_003014"
     CDS             5265..6155
                     /locus_tag="KRL67_003014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HBH1523880.1"
                     /translation="MSSTIKSNRIKGIMFIIASALGFAMMSAFVKLAGDLPSFQKVFF
                     RNLVSAIIAFWFIIKHKGSLTGKKENLKVLLYRSIFGTLGVIFNYYAIDKLMLSDANM
                     LNKMSPFLVVIFCAIALKEKINVKQIGAIIIAFIGALFIIKPTFSVEVIPYLGGIAGA
                     VFAAMAYTCLRVLGDREDYYTIVFFFSVFSLVTVGPIAFAVYEPMTLMQLIYLLLGGV
                     FASLGQFGITLAYKYAPAKEISIFDYSNIIFSAILSIFLFNVYPDMLSVVGYLIVFSA
                     AFYMFLYNKKLDKLDKEKKK"
     gene            6373..7659
                     /locus_tag="KRL67_003015"
     CDS             6373..7659
                     /locus_tag="KRL67_003015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HBH1523881.1"
                     /translation="MEKVKVAVIGAGNRGTYAYAPYIYENSDVCEIVAVAEPKKGRRE
                     LFTQKYNLDSKNVFETLEEFFKHDKMADAVIIATNDDRHYDVAKLALEKGYHVLLEKP
                     MSNSLDGLVHINDLCDKYKDKIFMICHVLRYSPFYNKLKEIVESKKLGELVSIQYNEN
                     IGYWHFAHSFTRGNWRNSNETSPLILAKSCHDMDILLYLVGSRCKKISAFGSLKHLNN
                     QNASGEMAQNCLQCLVEKKCPYSAKRIYLEKDRSINRAVHINPTEENLLNILKTSPYG
                     RCVYRCDNNVVDNMVNILQFENGVTATFNLCAFTKENGRTIKLMFSHGEVGGDLNKNE
                     IRIKEFGKNEEIVMNPSNQQNMVEEYDRNLIAEFIKLVSNKELEKGRVAAKEAIQSHV
                     MAFAAEYSRVSDEVVYIEEFFDSAKQMTKEIEETIF"
     gene            complement(7748..8191)
                     /locus_tag="KRL67_003016"
     CDS             complement(7748..8191)
                     /locus_tag="KRL67_003016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory YrvL family protein"
                     /protein_id="HBH1523882.1"
                     /translation="MIKISNKRRIPLGEFISVFLFFAVFFGITFCIFTVIGIGFLSFL
                     GFEYKSLGAVLIFFLIYFCITTPIDFLCTTILDIFRYVNKLPYSIYKLCEFIIDFILT
                     FLAMNIIDTFMDSVTIPLSTEILFALLSYLLSECMDFFDRDKKKP"
     gene            complement(8215..9009)
                     /locus_tag="KRL67_003017"
     CDS             complement(8215..9009)
                     /locus_tag="KRL67_003017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M55 family metallopeptidase"
                     /protein_id="HBH1523883.1"
                     /translation="MKLYLSADIEGTCGIVNWDETKLECEFSQHYRTQMSKEITAACD
                     GATQSGVHEILVKDAHDSGRNINPNILPENVKILRGWTKDPLVMMAGIDESFDACIFT
                     GYHSGATCNGNPLSHTMDIDYDYFKINDEIASEFTINAYIAAYYNVPVAFLSGDEMLC
                     ESAKKLNPNIVTVPVSKGIGNGSISIHPNLALKKIEKGVKKALSGDLSRHLIKLPDKF
                     KIEIKFREHYKAFKASFYPNMKKIDSQTVLFETEDYYEFLRMLLFI"
BASE COUNT         3601 a         1069 c         1614 g         3126 t
ORIGIN      
        1 aaaagagtaa attttcagta aattatcttg acatatatat tgaagtaata atataattta
       61 aagtataaaa ttttaaaaat tgaattataa cttcaaaaaa taaggagggt aatatgaaga
      121 taacagatat caaatttgaa aagcttagaa ttaaattaaa aaaacctgta gtagtttcat
      181 ttggagtaat tgaatatgga gaaagtataa ttttaaaaat tgaaactgat gaagggtatt
      241 gtggatttgg cgaagcagca cctcttgcag cagttacagg tgaagttttg gataatgttc
      301 tttcagtatt gcttatgttt aaaaaagagt taataggcaa agacccacta gatattgaaa
      361 ccatacatgc aatcatggat ggaattatta taggtaatac ttctgcaaaa gcagctattg
      421 acatagcttt atatgatatt aaaggaaaaa ttatgaatgc acctctttat aaggttcttg
      481 ggggatttga taataaagtt caaacagata ttactataag tatagataag ccagaaaaaa
      541 tggcagtaga agctttagaa agagtaaaag aaggttttag gatattgaag cttaaagcag
      601 gtataaatcc tgaagacgat attgaagctg taaagttaat tagaaaagct gttggagaga
      661 gtataagaat tagaattgat gcaaatcaag gatggaatgt aaatagcagt ataaatacta
      721 tcaaaaagtt agaagaattt aatgttgatg caattgaaca agcacttcca cattgggatt
      781 tagatggtac tgcttacata agaaataaaa gtaatacaaa aattatgata gatgaatcat
      841 tacattcacc aatagatgca attaaggcta taaaaaagaa tgcagttgat acatttaata
      901 ttaagctgat gaaatcaagt ggaatatatc ctgctataaa aatcaacaac atagctgagg
      961 caagtggagt aaattgtatg ttaggatgta tgctggaaac tagaattggt ataacagcag
     1021 cagcaaattt aatagcatct aaaaagaaca taacagaagc tgaccttgat agttttatgt
     1081 tttgtgaaga attagaaggt atatctggag gatttgtaat ggatggagat attatgaatc
     1141 ttgtcaataa accaggttta ggaatagaag taaacttatg ataaatttgg aggttaaata
     1201 tgaatacact tttttcaaat ccaggtagta tacttgctgt aatgacatca atgattgcat
     1261 tgggttttta cttacaaaga tacaaagcca ttaaaagttt aggaccagca cttacaataa
     1321 tcataatggg aataatactt tcaaacttaa aagtagttcc agtaagtact gagttatatg
     1381 gaactatttc tacatatgct attccagttt ctatgactat aatgttgatg agtgttgatt
     1441 taaaagaaat gacaaaatta tcaagagaac ctttgattgc aatttttgta gcagtattaa
     1501 ctgtaagtat catggcattt ttatttggac tagtatttgc agaaaaaata tcagaaggtt
     1561 ggaaagttgc tgggatgttt gtgggtacat acactggtgg aagtgctaat cttacagcta
     1621 ttggaacagg ccttaatgta agtagacaaa ctcttgctgc agcaaatgca gcagactatg
     1681 taattggagt accaacattg atatttatgt ttgctctacc agctatacta aaaaactcaa
     1741 agaagtttaa aaagctttgg ccatatcatg tagaagaatc agaattagag gattgtcaaa
     1801 atgaagaatt tatggagtca aaagagtgga gtattaaaga tatagcttgg atgttggcta
     1861 taggttttgt tgttacagag gttgcaacaa tacttgctgg atattttaat tcgagtttta
     1921 gtagtgcagc aagaatactt ttagttacta ctatttcgat tataattgct caattaaagc
     1981 cagtaaagaa actaaaggga aatttagatt taggattgtt tgttgcattg ttctttttat
     2041 gtactatagg tttttcagta gatataaaag agtttttagg ttcaactttt accataacac
     2101 tttactgttt tagcataatt tttgcttcat ttattttcca tttaggcata actagacttt
     2161 taaagataaa gtatcaatat gtaatacttt ctatagtagg agcaattgct gatggtccta
     2221 cttcagcttt agtagctgct tctgcaaagt ggaattcact tgtaagtgta gcagtcgtaa
     2281 tgggagtaat tggtggagta ttaggaaatt atgcaggtat atcagtggca tatgcaataa
     2341 agatgtctct gggattatag agggggagtt taattgataa agtttaaaat atatatatct
     2401 ggattatata gtggaaatgt aatctttggt gacgatttat taattgaaaa gttgaatcct
     2461 tttacaaata aaatagaaag tttaaaacca ataagcagag aagaaaatac atactattta
     2521 aatcttacaa aaatagattt aaagtcttta tttaagaact ttgatgtata tactaaaagt
     2581 ttagtaaata cagataaaga cacagttata aataacttag gagaaacgca ttctaaactt
     2641 aatgaatata tatgggttca aagaaataaa aagtttccac tagatataat tattgtagat
     2701 aataaaatag taggttttat ttgtttatcc agagaaactt gtactatatt aattatggat
     2761 ggatatgaag agtatactgt attaaaagaa tgggataaaa ctcataaaga tgaagagata
     2821 tattctataa gatttggtgg aaattatatg gttgatatga aagatggtat aaaactttct
     2881 acagatgtat atctccctaa ttttatagat tctaccaaaa aagcaccaac aattcttatg
     2941 agaactccat atggaaaaga aaatgataaa gaaatatatt ataaatatgt acaaagagga
     3001 tatgctgttg taatacagga tgttagagga agaaacgaat cagaaggtaa gtgggaacct
     3061 atgattcatg aaagagaaga tggagatagt actataaact ggatagtatc acaggaatgg
     3121 tcagatggaa cagttggaat gcttggagca tcatatcttg gctatgttca gtgggcagct
     3181 gcatcttcag gtaataagca tcttaaagct ttagttagta tagtaacatc aggaagccct
     3241 tttattgata taccaagaaa aggaggagct ttcgtatctg gtatgcttgc atgggcgttt
     3301 atggtatcaa gaaataaagt tgatagaagt aaaatggtaa gagatgattg ggatgatgtt
     3361 ttaaacatac gaccaataga gaatatacct gttgaagctc ttggatatag aattgaattt
     3421 ctagaagaat ggttaaagag agtagaaaaa gatgagtatt gggatttgat ggattggcat
     3481 ttacagaaag ataaaataaa tgtacctgcg ttggttgtat ctgggtggta tgatgataat
     3541 ggtatgggaa ctacagaggc cttagatgtt ataaaggact atgaaaaagg aaagagaaag
     3601 gctattctag gtccatggat gcataattca aatactttaa gagatataaa tggtatttct
     3661 ttaggaaata gttcattaag atatgatttg gattataatt atctattatg gtttgataaa
     3721 tacttaaaag gtatagaaaa taacatagat acgactgctc cagtagagta ttactctgta
     3781 ggttctaata agtggaagac agaagaaaat tggccaataa taaataagat agatagaagt
     3841 atgtatctaa taagtgatgg aaatgctaac acatctttag gaaatggtag attagtattt
     3901 gatagtgatt tagaagaaaa gtatgatagt tatatatata atccaaaaga cccatcagta
     3961 caactaattg atatgtcaga gaatgaagtt ggagttccaa atagctacaa agatttagaa
     4021 aaaagaagtg acatgttgtg ttatacatca gatgctttta gtgaagaatt tactgtaaca
     4081 ggtgatataa agttagagtt ttttgcatca agttccgcca aagatactga ctgggttata
     4141 aaaattatgg acgtggattt agatggaaat tcaattaaat tagctgatgg tatacttagt
     4201 gctagattta gaaatagctt ttataagtca gagtttatgg aagaaggaga gatttataag
     4261 tttacgatta taacttcaaa aatatcaaac acgtttaaag ttggtcataa aattagacta
     4321 gatataacat ctagtgctaa aaactttatt ttccaaaata gcaacacgac agagggatat
     4381 aatagtattg aatatataga agcaaagaat acgatttacc atggaggtaa atatccatct
     4441 aaattggtat tgccaataga gaataagtaa atttatatag attaaatagt gaggaggtgt
     4501 ctcaaaatga attttttagt tcgtgaggca ctttctttta tatgaaatta aatatatttt
     4561 catcatttca acaatgaaaa agaggcttat ctctctattt tgagatagcc tcctataagc
     4621 taaattgatt attttgtagc tgcaacaacg ctcatttcaa ctaacaaatg ctctctagca
     4681 agtctagctt caacacaagc tctagcaggt tcaaatcctt tttcaaccca agcatcccaa
     4741 acagagttca tagcttcaaa atctttcata tctcttaaat aaatagtaac agatagtaaa
     4801 tgttgcttat ctgaaccata tttgtttaat aaatcttcta ttttagccaa aacttcagtt
     4861 gtttgttcaa ttactcctcc ttcagcatga gtttgtccac ataaataaac tgtattgtta
     4921 tgaactacag ctctactcat tcttccagtt ccttcatatc tctttatttc catattttaa
     4981 atacctccat agttaaaaaa tctattaaaa atctaaataa taaattaaaa aaagtctata
     5041 ctatataaaa ttatattata tattctgata ataatccata aaaacatata actttttaac
     5101 tataattatt attgttatta attgaaaaga tttattagtt acttgacata gtttacactt
     5161 ttaattatag tataaattgg aatatttaga taattattat ttgatgtata agctaagttt
     5221 ttaattaata tgactttata catttatata gaaaagaggt acgtatgagt agcacaatca
     5281 aatcaaatcg aataaaaggt ataatgttta taatagcatc agcgcttggc tttgcaatga
     5341 tgtctgcatt cgtaaagtta gctggagatt taccaagttt tcaaaaagtg ttctttagaa
     5401 atctggtatc agctataata gctttttggt ttatcataaa acataaagga agtctaactg
     5461 gcaaaaaaga aaatttaaaa gtacttttat ataggtcaat atttggaact ctaggagtta
     5521 tatttaacta ctatgcaatt gacaagttga tgctttcaga tgctaatatg ctaaataaaa
     5581 tgagtccatt ccttgtagta atattttgtg caattgctct aaaagaaaaa ataaatgtaa
     5641 aacagatagg agctattata attgcattta taggagcttt attcataata aaaccaacat
     5701 ttagtgtaga agtaattcca tatcttggag gaattgcagg agctgtattt gcagcaatgg
     5761 catatacttg tttaagagtt cttggagata gagaagatta ctatacaatt gtatttttct
     5821 tttcagtttt ttcattagtt actgtaggac caattgcttt tgcagtatat gaaccaatga
     5881 ctcttatgca acttatatat ctattactag gtggagtatt tgcaagtcta ggtcaatttg
     5941 gaataacttt agcatataaa tatgcacccg ctaaggaaat ctctatattt gactatagta
     6001 atataatctt ttcagcaatt ttaagtatat tcttattcaa tgtctatcca gatatgttaa
     6061 gtgttgttgg atatttaata gtattttcgg cagcatttta tatgttttta tataataaga
     6121 aattagataa attagacaaa gaaaagaaga agtaagctag ataaaagtgt ttttatagtt
     6181 tgtattaaat atttaagtct ttgtattaaa attaaagaaa atttaattaa aaatgtttgg
     6241 tatattaact tataacagca gttaaaattg aaaaattata actgctgttt cttttattaa
     6301 aaatatccaa atgtccgtaa aaatagacta taatagagta gtataaataa tataaagggg
     6361 ataaattaaa gtatggaaaa agtgaaagtt gctgtgattg gagcaggtaa tagaggaaca
     6421 tatgcatatg ctccatatat ttatgaaaat tctgatgtat gtgaaatagt tgctgtagca
     6481 gaacctaaaa aaggaagacg agaactattt acacaaaaat ataacctaga tagtaaaaat
     6541 gtatttgaaa ctttagaaga attttttaaa catgacaaga tggcagatgc agtaataata
     6601 gctacaaatg atgatagaca ttatgatgta gcaaaactag ctcttgaaaa aggttatcat
     6661 gtactattag aaaagccaat gtctaatagt ttagatggtt tggtacatat aaatgacttg
     6721 tgtgataagt ataaagataa gatttttatg atttgtcatg tactgagata ttcgccattt
     6781 tataataaat taaaagaaat tgtagaaagc aaaaaattag gagagctagt tagtatacaa
     6841 tataatgaaa atataggata ttggcatttt gcacatagct ttactagagg gaattggaga
     6901 aatagcaatg aaacaagtcc tctaatttta gcaaagagtt gtcatgatat ggatatttta
     6961 ttatatttag taggaagtag atgcaaaaaa atatctgcat ttggaagctt aaagcatctg
     7021 aataaccaaa atgctagtgg agagatggca caaaattgtc ttcagtgttt agttgaaaaa
     7081 aaatgtccat attcagcaaa gagaatttat ttagaaaaag atagaagtat aaatagagct
     7141 gttcacataa atccaactga agagaatcta ttaaatatac ttaagacaag tccatatgga
     7201 agatgtgttt atagatgtga caataatgtt gtagacaata tggtaaatat actacagttt
     7261 gaaaatggag tgacagctac ttttaattta tgtgcattta ctaaagaaaa tggaagaact
     7321 ataaagttaa tgtttagcca tggtgaagtt ggtggggatt taaataaaaa tgagataaga
     7381 attaaagaat ttggaaaaaa cgaagaaata gttatgaatc catcaaatca acagaatatg
     7441 gtagaggaat atgatagaaa tcttatagct gaatttatta aattagtttc aaataaagaa
     7501 ctagaaaagg gtagagtagc agcaaaagaa gctatacaaa gtcatgtaat ggcatttgca
     7561 gcagaatatt ctagggtcag tgatgaagta gtgtatatag aagaattttt tgatagtgct
     7621 aagcaaatga ctaaggagat agaagaaact atattttaat taataaaaat ttattaaata
     7681 aaataaagat agattaaata atagaaatac gaaaatatgt gttctattaa ggaatctatc
     7741 ttttttatta aggttttttc ttatccctat caaaaaaatc catacattct gataaaagat
     7801 aagaaagtaa agcgaataag atttctgtac ttaaaggaat cgttactgaa tccataaagg
     7861 tatcaattat gttcatagct agaaaagtta gtataaaatc aattataaat tcgcaaagtt
     7921 tatatattga gtatggtaat ttgtttacat atctgaatat atctaaaata gtagtgcata
     7981 gaaaatcaat tggagtggtt atacaaaaat atattaaaaa gaaaattaat acagctccaa
     8041 gagatttata ttcaaatcct aagaatgata agaagccaat tcctataacc gtgaaaatac
     8101 aaaaagttat accaaaaaaa acagcaaaaa ataaaaatac agaaataaat tcgcctaaag
     8161 gaatccttct tttattactt atcttaatca actccttaat ttatgatatg aaatttatat
     8221 aaataataac attcttaaaa attcataata gtcttctgtt tcaaatagta ctgtttgaga
     8281 atctattttt ttcatatttg gataaaaaga ggccttaaaa gctttataat gttctctaaa
     8341 ttttatttct atcttaaatt tatctggcaa ttttattaag tgcctagata aatctccaga
     8401 taaagctttt ttgacacctt tttcaatttt ttttagtgca aggtttggat ggattgaaat
     8461 tgaaccattt ccaattcctt ttgacacagg aacagtgact atatttggat ttagtttttt
     8521 tgcactctca caaagcattt catctccact taaaaatgct acaggaacat tataatatgc
     8581 agcaatataa gcatttatag taaattcaga tgctatttca tcatttattt taaaatagtc
     8641 ataatcaata tccatagtat gggaaagtgg attaccatta caagtagctc cagagtgata
     8701 gccagtgaat atacatgcat caaagctttc atcaattcca gccatcataa ctagtgggtc
     8761 tttggtccat cctctaagta ttttgacgtt ttcaggtaaa atatttggat taatatttct
     8821 accactgtca tgtgcatctt taacaagtat ttcatgtaca ccagattgag tagctccatc
     8881 acatgctgct gttatctctt tactcatttg tgttctatag tgttgtgaaa attcacattc
     8941 aagttttgtt tcatcccaat ttacaatacc acaagtacct tctatatcag cacttaaata
     9001 caatttcata atatataccc ccttatgttt attattgatg ataataacag ataacagaaa
     9061 atacataatc aataaagggc ataaaaatat tatgtcttac tattaattat tattaaatct
     9121 gtaactgaaa aataactaaa ttggtattaa aaaatataac actatgtgtc atttatttat
     9181 atagtttttt atatctgatg tttatataaa attgtaacat ataagtggtt acatttacta
     9241 gtttaacata gttaaaatag ttaggtatta tttttaagtt aaattgatta aaaaatagct
     9301 acgcacactc gtgactttag tcataagtta gtagctaaaa tagtcagcat atagggaaac
     9361 ctatatgtat agataggata gaacctatcc aatactactt gaattgctgg
//
LOCUS       DAEPXF010000154         9312 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain HB27
            SAMN13376389-rid12479423.denovo.161, whole genome shotgun sequence.
ACCESSION   DAEPXF010000154 DAEPXF010000000
VERSION     DAEPXF010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13376389
            Sequence Read Archive: SRR10518432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 9312)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9312)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001069624.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001069624.1
            Genome Coverage       :: 315x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/15/2021 01:54:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,165
            CDSs (total)                      :: 4,076
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 89
            rRNAs                             :: 10, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 10 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 19 of 69
            Pseudo Genes (incomplete)         :: 44 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 10 of 69
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9312
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13376389-rid12479423.denovo.161"
                     /strain="HB27"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="China: Hebei"
                     /collection_date="2017"
     gene            51..1811
                     /gene="prdR"
                     /locus_tag="KRL67_003018"
     CDS             51..1811
                     /gene="prdR"
                     /locus_tag="KRL67_003018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator
                     PrdR"
                     /protein_id="HBH1523884.1"
                     /translation="MFSIPEVKKVEEVMDTKFTTIDEDTRIEDAIKEMIKSNTKTLMV
                     IDSSDQLKGIISMTDIHNLYEMHKKYEGQPVKLIMKKDVIYVNEGLTLDECRDIMILK
                     NIGILPVLRDNKIIGVLKQEHIRDYLYMHLEDYGLTLKYIIGQIKEGICAINNEGVVI
                     LWNKFMEERYDIKSEDIVGRPMNEFLENTISEKVLNSKVGMSDLYFTDKKENMYALVH
                     ANPIFYKEEFIGVVCTEVDVTEAKILALELEKVNDTLKYLKNEVKNLSKGSFDKILGK
                     SYKLEKSKAIAKQVARTNSSIFIWGESGTGKEVFARAIHDYSERKGQFIPVNCSAIPN
                     ELFESEFFGYESGAFTGASKKGRIGIFELAKDGTVFLDEIADLPLSMQAKLLRVLQEK
                     EIRRVGGDTTIKINPRIISATNKDLSKMVKAEKFREDLYYRLNVVEIKIPPLRERKED
                     IGLLVHSFLEEICKQNNKPVLTISKDVIDIFQNYRWKGNIRELKNTIENIVVLSQNSK
                     IDVDDVPSYMMDSTNNSTEEEEYPLDLTKATQKIEIKNITKALKMSNGNKAKAAKILN
                     IPRTTLYYKIDQYKIDVSKI"
     gene            2240..4120
                     /gene="prdA"
                     /locus_tag="KRL67_003019"
     CDS             2240..4120
                     /gene="prdA"
                     /locus_tag="KRL67_003019"
                     /EC_number="1.21.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-proline reductase (dithiol) proprotein PrdA"
                     /protein_id="HBH1523885.1"
                     /translation="MSITLETAQAHANDPAVCCCRFEAGTIIAPENLEDPAIFADLED
                     SGLLTIPENGLTIGQVLGAKLKETLDALSPMTTDNVEGYKAGEAKEEVVEETVEEAAP
                     VSEAAVVPVSAGVAGETVKIHIGEGKNISLEIPLSVAGQAGVAAPVANVAAPVASAAA
                     EVAPKVEEKKLLRSLTKKHFKIDKVEFADETKIEGTTLYIRNAEEICKEANETQELVV
                     DMKLEIITPDKYETYSEAVLDIQPIATKEEGELGSGITRVIDGAVMVLTGTDEDGVQI
                     GEFGSSEGELNTTIMWGRPGAADKGEIFIKGQVTIKAGTNMERPGPLAAHRAFDYVTQ
                     EIREALKKVDNSLVVDEEVIEQYRREGKKKVVVIKEIMGQGAMHDNLILPVEPVGTLG
                     AQPNVDLGNMPVVLSPLEVLDGGIHALTCIGPASKEMSRHYWREPLVIRAMEDEEIDL
                     VGVVFVGSPQVNAEKFYVSKRLGMLVEAMEVDGAVVTTEGFGNNHIDFASHIEQIGMR
                     GIPVVGVSFSAVQGALVVGNKYMTHMVDNNKSKQGIENEILSNNTLAPEDAVRIMAML
                     KNAIEGVEVKAPERKWNPNVKLNNIEAIEKVTGEKIVLEENEQSLPMSKKRREIYEKD
                     EN"
     gene            4201..4485
                     /locus_tag="KRL67_003020"
     CDS             4201..4485
                     /locus_tag="KRL67_003020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH1523886.1"
                     /translation="MDRLKYISSETFYEGIIVDIKGGGVTIDLKGRLGQFKIPNRMLI
                     TDYELKIGQEVGFMLSYPEVLSPEPNQEYVENIRREKEKQAEMQSKQEEK"
     gene            4523..5248
                     /gene="prdB"
                     /locus_tag="KRL67_003021"
     CDS             4523..5248
                     /gene="prdB"
                     /locus_tag="KRL67_003021"
                     /EC_number="1.21.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011986824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:4973..4975,aa:Sec)
                     /transl_table=11
                     /product="D-proline reductase (dithiol) protein PrdB"
                     /protein_id="HBH1523887.1"
                     /translation="MSLTTVQGLQSEIFVPITPPPVWTPVTKELKDMTIALATAAGVH
                     LKSDKRFNLAGDTTFRAIPNTATVDEMMVSHGGYDNGDVNKDINCMFPIDRLHELAAE
                     GFIKGVAPMHYAFMGGGGNQHVFTEETGPAIAAKLKEEGVDGVVMTAGUGTCHRTAVI
                     VQRAIEEAGIPTIIIAALPPVVRQNGTPRAVAPLVPMGANAGEPHNIEMQTHILRDTL
                     EQLVAIPSAGKIVPLPYEYKAHV"
     gene            5328..6086
                     /gene="prdD"
                     /locus_tag="KRL67_003022"
     CDS             5328..6086
                     /gene="prdD"
                     /locus_tag="KRL67_003022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline reductase cluster protein PrdD"
                     /protein_id="HBH1523888.1"
                     /translation="MEEKIMRRLVIKPFHMNEVNFGSKTSIKKDVLTIDLSSIDEIKE
                     REELITDIKVDIIKPGDYDREINTIMDIIPISTKVLGRLGEGITHTLTGVYVMLTGAD
                     EDGNQMHEFGSSEGILKDQMVFGRYGTPSVEDYIIHVDVTLKGGLPFERTLPLAAFRA
                     CDDFIQEIRASLKMEDGRNATQVREYFDKIRPNAKKVVIVKQIAGQGAMYDNQLFSKE
                     PSGFEGGTSIIDMGNVPMIISPNEYRDGALRAMT"
     gene            6101..6568
                     /locus_tag="KRL67_003023"
     CDS             6101..6568
                     /locus_tag="KRL67_003023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline reductase"
                     /protein_id="HBH1523889.1"
                     /translation="MGIGPSTKETSLHHFRDPLLDIVGSDKDVDLLGVIVVGTPDGNE
                     NKTFVGQRTAAWLEAMRVDGAIVSSDGWGNSHVDYANTFEEIGKRDIPVVGVTFNGTQ
                     AKFVVSNQYMDTIVDMNKSKEGIETEVVGENNTNEIDAKKALAFLKLKMRKHG"
     gene            6586..7068
                     /locus_tag="KRL67_003024"
     CDS             6586..7068
                     /locus_tag="KRL67_003024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline reductase"
                     /protein_id="HBH1523890.1"
                     /translation="MSFVGISTSMKNTSLHIFRDPLLDLIDNDSDIDLLGVVVVGTSE
                     HNEWKTFLATRLGRWIESLRPDGVIITLDMAGNQHIDFTNAIAEFVKSDIPTVGLTIM
                     GADGLVITNPYLDKATIIDYKKTTGYIETEVVGDNHMDEVDVKKALAFLKLKMRKDAK
                     "
     gene            7088..8095
                     /locus_tag="KRL67_003025"
     CDS             7088..8095
                     /locus_tag="KRL67_003025"
                     /EC_number="5.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline racemase"
                     /protein_id="HBH1523891.1"
                     /translation="MKFSRSIQAIDSHTAGEATRIVVGGIPNIKGNSMPEKKEYLEEN
                     LDYLRTAIMLEPRGHNDMFGSVMTQPCCPDADFGIIFMDGGGYLNMCGHGTIGAMTAA
                     IETGVVPAVEPVTHVVMEAPAGIIRGDVTVVDGKAKEVSFLNVPAFLYKEGVEVDLPG
                     VGTVKFDISFGGSFFAIIHASQLGLKIEPQNAGKLTELAMKLRDIINEKIEIQHPTLA
                     HIKTVDLVEIYDEPTHPEATYKNVVIFGQGQVDRSPCGTGTSAKLATLHAKGELKVGE
                     KFVYESILGTLFKGEIVEETKVADFNAVVPKITGSAYITGFNHFVIDEEDPLKHGFIL
                     K"
     gene            8215..9096
                     /locus_tag="KRL67_003026"
     CDS             8215..9096
                     /locus_tag="KRL67_003026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TSUP family transporter"
                     /protein_id="HBH1523892.1"
                     /translation="MVAILKALYVVFAAIFAVVFGKDVAKANKEGKLEEQSTPKVAVT
                     GFITDFFDTLGIGSFAPTIAMSKALKLNIPDKKMPGTLNVAHTIPVVTEAFIFTSIIP
                     VDGVTLVSMVVAAAVGSYIGAGIIAKMDERKIQLVMGVTLALTAILMLLGHPWINVLP
                     GGGNELGLTGAKLVIGVIGNFILGALMTAGIGLYAPGMAMVYFLGMSAKVAFPIVMGS
                     CALLMPVASMKFIKEDAYTKKASVIIAICGLIGVFVAAYIVKTLPMDILKALVIVVIA
                     YTSATMIMAANKNKKTA"
BASE COUNT         3646 a         1146 c         1872 g         2648 t
ORIGIN      
        1 aatatatata aataagtttt gatgaggtac tactataaag gggggacttt atgttttcga
       61 taccagaagt aaaaaaagta gaagaggtaa tggatacaaa atttacaact atagacgaag
      121 acacaagaat tgaagatgca ataaaagaaa tgataaaaag caacacaaaa actctaatgg
      181 taatagattc atctgaccaa ttaaaaggca ttatatccat gacggatata cataatcttt
      241 atgagatgca taaaaagtat gaaggtcaac cagtaaaact tataatgaag aaggatgtaa
      301 tatatgtcaa cgaaggttta accttagatg aatgtagaga tataatgata ttaaaaaata
      361 tagggattct tcctgttctt agagataata aaattattgg agttctaaaa caagagcata
      421 ttagggacta tttatatatg catttagaag attatggatt gactttgaag tatattatag
      481 gtcaaattaa agaaggaatt tgtgctataa ataacgaagg tgttgttatc ttgtggaata
      541 agtttatgga ggaaaggtat gatataaagt ctgaagacat agttggtcga ccgatgaatg
      601 aatttttgga aaatactatt tcagaaaaag tactaaatag taaagttgga atgagtgact
      661 tgtattttac agataaaaaa gaaaatatgt atgcattagt tcatgcaaat cctatatttt
      721 ataaagaaga atttatcggt gttgtatgta cagaagttga tgttacagaa gctaagatat
      781 tagctcttga acttgaaaaa gtaaatgata cattaaaata tttaaagaac gaagtcaaaa
      841 atttatctaa aggtagtttt gataagattt taggtaaaag ttataaactg gaaaaatcga
      901 aagcgattgc aaaacaagtg gctagaacaa atagtagcat atttatttgg ggtgaaagtg
      961 gtacaggtaa agaggtattt gcaagagcta ttcatgatta tagtgagaga aaaggtcaat
     1021 ttatacctgt taactgtagt gctataccaa atgagttatt tgaaagtgag ttttttgggt
     1081 atgaatcagg tgcatttaca ggagcgagta aaaagggtcg aataggtata tttgaacttg
     1141 caaaagatgg aactgtattt ttagatgaaa ttgccgattt acctcttagt atgcaagcaa
     1201 agttgcttag agtattacag gaaaaagaaa taagaagagt tggtggagat actacaataa
     1261 aaataaatcc tagaataata tcagctacca ataaagattt gagtaaaatg gtaaaggcag
     1321 aaaaatttag agaagattta tactatagat taaatgttgt tgaaataaag attcctccac
     1381 ttagagagag aaaagaagac ataggattat tggttcatag ctttttagag gagatatgta
     1441 aacaaaataa taagcctgtt cttacaatta gcaaagatgt aatagatata tttcagaatt
     1501 acagatggaa aggtaatatt agagaattaa aaaatacgat tgaaaatatt gttgtattgt
     1561 cacaaaattc taaaatagat gtggatgatg taccaagtta tatgatggat tctacaaata
     1621 atagtactga agaagaagag tatcctttag atttaacgaa ggcaactcaa aaaattgaaa
     1681 taaagaatat aaccaaggca cttaaaatgt ctaatggaaa taaagctaaa gcagctaaga
     1741 tattaaatat acccagaaca actttgtatt ataagataga ccaatacaaa atagatgtgt
     1801 ctaaaatatg acacatgact aaattttgac aaaatgataa actttaattt aaaacctagt
     1861 aaagccaacg cttgtataag tgttgtgtat atagatgtgt taaaaaaaat gaaaaagtgt
     1921 tgattaattg acactttttt tgtttgagaa aatgtttaca aatacaaaaa ataacatatt
     1981 ttaaataaat atttatataa tatttggcta aaaatagcga tagatgaaga atctattata
     2041 gaaaaaatct ttttgttaat aagttggcat aggaattgct tataatataa agtgacttta
     2101 agtgagaaaa aaataatatt aatcataaca aattatgtag gttttaattt acttaatttc
     2161 tcagttaagg ggttacctgt tgaggaattg agatttaaaa taataaataa gaaaataatg
     2221 taaggaggta ttttatacta tgtcaataac tttagaaaca gctcaagccc atgcaaatga
     2281 cccagcagtt tgttgttgta gatttgaagc gggaacaatt atagcgccag aaaacttaga
     2341 agatccagca atatttgcag acttagagga ttctggatta ttaacaatac cagaaaatgg
     2401 attaactata ggtcaagtac taggagctaa gttaaaagaa actttagatg ctctttctcc
     2461 aatgactaca gataacgtag aaggatacaa agcaggagag gctaaagaag aagtagtaga
     2521 agaaacagta gaagaagcag ctccagtatc agaagcagca gtagttccag taagcgcagg
     2581 agttgcaggt gaaacagtta aaatacacat aggtgaaggt aagaacataa gcttagagat
     2641 acctttatca gtagctggtc aagcaggagt tgctgctcca gtagcaaacg ttgctgctcc
     2701 agtggcaagt gcagcagcag aagtagctcc aaaagttgaa gaaaagaaac ttttaagaag
     2761 cttaactaaa aaacacttta aaatagataa agttgaattt gctgatgaaa ctaaaataga
     2821 aggaactact ttatacatca gaaacgcaga agaaatatgt aaagaagcta atgaaactca
     2881 agagttagtt gtagatatga agttagaaat aataactcct gataaatatg aaacttacag
     2941 tgaagctgta ttagatatac aaccaatcgc tactaaagaa gaaggcgaat taggttcagg
     3001 tataactaga gttatagatg gagctgtaat ggtattaact ggtacagatg aagatggagt
     3061 tcaaataggt gaatttggtt cttcagaagg tgagttaaat actactataa tgtggggtag
     3121 accaggtgct gctgacaaag gtgaaatatt catcaaaggt caagtaacaa taaaagcagg
     3181 aactaacatg gaaagaccag gacctttagc tgctcaccgt gcatttgact atgtaactca
     3241 agaaataaga gaagcattaa agaaagttga caactcttta gtagttgatg aagaagtaat
     3301 agagcaatac agaagagaag gtaaaaagaa agttgttgtt ataaaagaaa taatgggaca
     3361 aggtgcaatg catgataacc taatattacc agttgaacca gttggtacat taggagctca
     3421 accaaacgtt gacttaggaa acatgccagt tgtattatct ccacttgaag tattagatgg
     3481 tggtatccat gcattaactt gtataggacc tgcatcaaaa gaaatgtcaa gacattactg
     3541 gagagagcca ttagtaataa gagctatgga agacgaagaa atagatttag taggtgttgt
     3601 atttgttggt tctccacaag taaatgctga gaaattctat gtatctaaga gattaggtat
     3661 gttagttgaa gctatggaag ttgatggagc tgtagtaact actgaaggtt tcggaaacaa
     3721 ccatatagat ttcgcatctc acatagagca aataggtatg agaggtatac cagtagttgg
     3781 tgtaagtttc tcagctgttc aaggtgctct agttgttggt aataaataca tgactcacat
     3841 ggtagacaac aataagtcta aacaaggtat agagaatgaa atattatcta acaacacttt
     3901 agctccagaa gatgctgtta gaataatggc tatgcttaaa aatgctatcg aaggtgtaga
     3961 agttaaagct cctgaaagaa aatggaatcc aaatgttaaa ttaaataaca tagaagctat
     4021 agaaaaagtt acaggagaaa aaatagtatt agaagagaat gagcaatctc taccaatgag
     4081 taagaagaga agagaaatat acgaaaaaga cgaaaactaa aatttagata ctaactaaga
     4141 tttagacaat aacataaatt tagatccaag tttaaaacag tatagagagg tagaattata
     4201 atggataggt taaaatatat ctcatcagag actttttatg aaggtattat agttgacatc
     4261 aaaggtggtg gagtgactat agacctaaaa ggtagacttg gacaatttaa aatacctaat
     4321 agaatgctta taactgacta cgaacttaag ataggtcaag aagttggatt catgctaagc
     4381 tatcctgagg tattaagtcc agagcctaat caagagtatg tagaaaatat taggagagaa
     4441 aaagaaaaac aagcagaaat gcaatcaaaa caagaagaaa aataataaat aatcgtttga
     4501 aaatagaagg gagaggaaat atatgagcct tacaacagta caaggacttc aatctgaaat
     4561 attcgttcca ataacacctc ctccagtttg gactcctgta actaaagaat taaaagatat
     4621 gactatagct ttagctacag cagctggtgt tcatttaaaa tctgacaaga gatttaatct
     4681 agcaggagat actacattta gagctatacc taatacagct acagttgatg aaatgatggt
     4741 atcacatggt ggatatgaca atggagatgt aaacaaagat ataaactgta tgttccctat
     4801 agatagatta catgaattag ctgcagaagg atttatcaaa ggagtagctc ctatgcacta
     4861 tgcattcatg ggtggtggag gaaaccaaca tgtcttcact gaagaaactg gtcctgctat
     4921 cgctgctaaa cttaaggaag agggagtaga cggtgtagtt atgacagctg gctgaggtac
     4981 ttgccataga actgccgtga tcgtgcagag agcaatagag gaagctggaa tacctacaat
     5041 aataatcgca gctcttcctc cagtagttag acaaaacgga actcctagag cagttgctcc
     5101 attagttcca atgggtgcta atgctggtga accacataat atagaaatgc aaacacatat
     5161 attaagagat acattagagc aattagttgc aataccatct gctggtaaga tagttccatt
     5221 accatacgaa tataaagctc acgtttaaaa tgtaatttat atttgaaaat atagaaccct
     5281 tctcctacac agttaggaga ggggttttaa gaacaaaggt ggatattatg gaagaaaaaa
     5341 taatgagaag acttgttata aaaccgtttc atatgaatga agttaatttc ggttcaaaaa
     5401 cttctataaa gaaagatgta cttacaatag atttatcaag tatagatgaa ataaaagaaa
     5461 gagaagagtt aataacagac ataaaagtag acattataaa acctggagat tatgacagag
     5521 aaattaatac tattatggac attataccaa tatcaacaaa agttttaggc agactgggag
     5581 agggtattac acatacttta acgggtgtat atgtaatgtt aactggtgca gatgaagatg
     5641 gaaatcaaat gcatgaattt ggttcttcag aaggaatttt gaaagaccaa atggtatttg
     5701 ggagatatgg aactccatca gttgaagatt atataatcca tgtagatgta actttaaagg
     5761 gaggtttacc ttttgaaaga acacttccac ttgcagcatt cagagcatgt gatgacttta
     5821 tacaagaaat aagagcgtct ttgaaaatgg aagacggaag aaatgctact caagttcgtg
     5881 aatattttga caaaattcga ccaaatgcaa aaaaggttgt catagtaaaa caaatagcag
     5941 gtcaaggtgc tatgtatgac aatcagttat tctcaaagga acctagtgga tttgaaggag
     6001 gaacatcaat tatagatatg ggaaatgtac ctatgattat atctcctaat gaatacagag
     6061 atggtgccct tagagctatg acttaagaga ggtggatatt atgggaatag gaccttcaac
     6121 aaaagaaaca tctcttcatc actttagaga cccactttta gacatagttg gaagtgataa
     6181 ggatgtagat ttacttggag taatagtagt aggaactcca gatggaaatg aaaataaaac
     6241 ctttgtggga caaagaacgg ccgcttggct ggaagctatg agggtagatg gagctatagt
     6301 ttcatcagat ggttggggaa attcacatgt tgactacgct aacacatttg aggaaatagg
     6361 aaaaagagat atacctgtag ttggagttac atttaatggg actcaagcta agtttgttgt
     6421 aagcaatcaa tatatggata ccatagttga tatgaacaaa tcaaaagaag gtattgagac
     6481 agaagtagtt ggagaaaaca acacaaatga aatagatgct aaaaaagctt tggcattttt
     6541 aaaacttaaa atgagaaaac atggataaaa aatttaagta agggaatgag ttttgtggga
     6601 atttctacat caatgaaaaa cacatcactt catatattta gagacccatt actagatttg
     6661 atagataatg attctgacat agacttgtta ggagtggttg tagttgggac atcagagcac
     6721 aatgaatgga aaacattttt agcaactaga cttggaagat ggattgaatc attgcgacct
     6781 gatggagtga ttataacttt agatatggca ggaaaccagc acattgattt tacaaatgct
     6841 atagcagagt ttgtaaaatc agatatacct acagtaggtc ttactattat gggagcagat
     6901 ggattagtaa ttaccaatcc atatttggat aaagctacca taattgatta taaaaagaca
     6961 acaggctaca tagaaacaga agtagttgga gacaatcaca tggatgaagt tgatgttaaa
     7021 aaagcattgg ctttcctaaa attaaaaatg cgtaaagacg caaaataaac ctaaggggga
     7081 atttgatatg aaatttagca gaagtataca agctatagac tctcatacag caggtgaagc
     7141 aactagaata gttgtaggtg gaatacctaa cataaaagga aattctatgc ctgagaaaaa
     7201 agaatattta gaggaaaatc tagattattt aagaactgct ataatgttag agccaagagg
     7261 acataatgat atgtttggtt ctgtaatgac tcaaccttgt tgcccagatg ctgactttgg
     7321 aataatcttc atggatggtg gcggatacct taacatgtgt ggccatggta caataggagc
     7381 tatgacagca gctatagaaa caggtgtagt tccagcagta gagcctgtaa ctcatgtagt
     7441 tatggaagct ccagcaggaa taataagagg agacgttaca gttgtagatg gaaaagctaa
     7501 agaagtatca ttcttaaacg taccagcttt cttatataaa gaaggtgttg aagttgactt
     7561 accaggtgtt ggaactgtta aatttgatat atcatttggt ggaagcttct ttgctataat
     7621 acatgcaagt caattaggtt taaaaatcga acctcaaaat gctggtaaat taactgaact
     7681 agctatgaaa cttagagata taataaatga gaaaatagaa atacaacatc caactttagc
     7741 tcatataaaa actgtagact tagttgaaat atatgatgag ccaactcatc cagaagctac
     7801 ttacaaaaac gtagttatat ttggtcaagg tcaagttgac agatctccat gtggaactgg
     7861 aacaagtgct aaattagcta ctttacatgc taaaggtgaa ttaaaagtag gagaaaaatt
     7921 cgtatatgaa agtatattag gaactttatt caaaggtgaa atagttgaag aaactaaagt
     7981 tgcagatttc aatgctgtag tacctaaaat aactggttct gcttatataa ctggatttaa
     8041 tcattttgta atagatgaag aagacccact taaacatgga tttattctta aataagtaat
     8101 aaataaattg ggaaattaaa atagaatagt agccatttat cggctactat tctatagctt
     8161 ttaggctata agattcagaa tatatgaaaa aataaaaaaa taggaggatg gattatggta
     8221 gcaatcttaa aggcattata tgtagtattc gcagcaatct ttgcagttgt gtttggtaaa
     8281 gacgtagcta aagcaaacaa agaggggaaa ctagaagaac aaagtactcc aaaagttgct
     8341 gtaacaggat ttataacaga cttctttgat acattaggaa tagggtcatt tgctccaaca
     8401 atagcaatgt caaaggcttt aaagttaaat ataccagata aaaaaatgcc tggtacatta
     8461 aacgtagcac atactattcc agttgttaca gaagcattca tatttacatc aatcatacca
     8521 gttgatggtg ttactttagt atctatggtt gtggcagcag cagttggttc atatataggt
     8581 gcaggaataa tagctaagat ggacgagaga aaaatacaac ttgttatggg tgtaacttta
     8641 gcattaacag caatattaat gttattaggt catccttgga ttaacgtatt accaggtgga
     8701 ggaaatgaat taggtcttac tggtgctaaa ttagtaatag gtgtaatagg taactttata
     8761 ctaggagctt taatgacagc tggtataggt ctatatgctc caggtatggc aatggtttat
     8821 ttcttaggaa tgtctgcaaa agttgcattc ccaatagtta tgggttcttg tgcattatta
     8881 atgccagttg caagtatgaa attcataaaa gaagatgcat atactaaaaa agcatcagtt
     8941 ataatagcta tatgtggttt aataggagtt ttcgtagctg catatatagt aaaaactcta
     9001 ccaatggata tattaaaagc attagtaata gtagttatag cttatacttc tgctacaatg
     9061 ataatggctg caaataaaaa caaaaagact gcttagttaa agactactta gtgtttatta
     9121 aatattacag ctaataataa agattataaa ctgctttaaa atattgaatt aatattttag
     9181 ggcagttttt tatttactaa attaatataa agcaattaac atgccaatta tgtaatatat
     9241 atagtgattt agtagattta atgtaaaaaa cgttgtattt ttaaagaata aagcataatt
     9301 taaagctaaa ta
//
LOCUS       DAEPXF010000155         9174 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain HB27
            SAMN13376389-rid12479423.denovo.162, whole genome shotgun sequence.
ACCESSION   DAEPXF010000155 DAEPXF010000000
VERSION     DAEPXF010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13376389
            Sequence Read Archive: SRR10518432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 9174)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9174)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001069624.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001069624.1
            Genome Coverage       :: 315x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/15/2021 01:54:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,165
            CDSs (total)                      :: 4,076
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 89
            rRNAs                             :: 10, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 10 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 19 of 69
            Pseudo Genes (incomplete)         :: 44 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 10 of 69
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9174
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13376389-rid12479423.denovo.162"
                     /strain="HB27"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="China: Hebei"
                     /collection_date="2017"
     gene            <1..400
                     /locus_tag="KRL67_003027"
     CDS             <1..400
                     /locus_tag="KRL67_003027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HBH1523893.1"
                     /translation="KGEYSYIYENKREESEIIKYYNKTDLLYLKNVVSNIVPSRDEFI
                     TIYKQALIKKEIDLDMVNIRGTFNVIPLKFFTILNVFRELNLLDFNLNYEKNSVLIRI
                     LPKPQKKLDLNESLILNNLKNLEKQYNSSY"
     gene            413..925
                     /locus_tag="KRL67_003028"
     CDS             413..925
                     /locus_tag="KRL67_003028"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HBH1523894.1"
                     /translation="MDLKNFIRNIDDFPKPGIDFKDVTTLFKDGDAFKYAVDSIVEEL
                     KDKDVDLVIGPEARGFLMGTPVAYALGVGFVPIRKPGKLPGEVESYEYGLEYGTDTLE
                     IHKDAIKKGQKVAIVDDLLATGGTMEAAAKLVEKLGGEVVSMQFLIELKFLNGREKLS
                     NYDVNSLIKY"
     gene            999..3206
                     /locus_tag="KRL67_003029"
     CDS             999..3206
                     /locus_tag="KRL67_003029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003229747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HBH1523895.1"
                     /translation="MHDKELQEIIEKIKVYAPNANFEMVEKAYNLAKKAHEGQYRKSG
                     EPYFIHPLAVANILIEMELDIETITAGLLHDVVEDTEYTYEDIKKEFGKEVADLVDGV
                     TKLGQIKYRSKEETQSENLRKMFLAMAKDIRVILIKLADRLHNMRTLKYMPPEKAKYK
                     AKETLEIYGGIAHRLGISKIKWELEDRALRFMDPEGYYDLVSRVSMKRSQREDYIQGI
                     VELLKDKFKDLSIPCDVYGRPKHFYSIYRKMQKKHKTFEEIFDLTAVRILVDNVKDCY
                     AVLGIVHTLWRPIPGRFKDYIAMPKPNMYQSLHTTVVGPDGEPLEIQIRTHEMHNIAE
                     NGIAAHWKYKEGISSKESKVEEKLQWLRQMMEWEKDLKDPQEFMDALKEDVFNSQVYV
                     FTPKGDVIELPAESTPIDFAYRVHTNVGNKCVGAKIDGRIVPIDYKLQNGNIVEVLTS
                     SNSNGPSRDWLNIVKTPNAKSRIRQFFKKERREENVDRGNTILEREFKKHDIPQKDPL
                     VEKYMALVAKKFNQPSVEDLIATVGYGGIMSSQVVSKVKDLYLKEVKKAKKEQQIQEE
                     DLNKHNISETEYSKKRKKNASQGIIVKGLDNILVRFAKCCNPLPGDEIVGYITKGRGV
                     AIHRADCPNANNNGDFFQNRLVEVSWSSAGKGKFEAEIQIKAIDRRGIVNEITHIVAM
                     DKLSLNGINARKGKDSIVSINLLVEVNDTEELKILMKKLKAIPGVESIYRMTN"
     gene            3231..3680
                     /locus_tag="KRL67_003030"
     CDS             3231..3680
                     /locus_tag="KRL67_003030"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003517065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-tyrosyl-tRNA(Tyr) deacylase"
                     /protein_id="HBH1523896.1"
                     /translation="MRAVVQRVSSSKVTVDENTIGQINKGLLVLLGVTHDDKSSDVDY
                     MIDKILNLRIFEDENDKMNLSLMDIGGELLVVSQFTLYGDCRKGRRPGFSNAARPELA
                     NNLYEEFVKKAKDKGVTVGTGQFAAHMMVELTNDGPVTILLDSSKSF"
     gene            3695..4309
                     /locus_tag="KRL67_003031"
     CDS             3695..4309
                     /locus_tag="KRL67_003031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HBH1523897.1"
                     /translation="MIIKKFVDSYFGVNTYVLGDEKTKKCAVIDPGGSLVETLSFVKE
                     NELNIEYIILTHGHGDHIGYVKDIKEKTGAKVVAHIAEKELLNDKNKNLSYTMRCGAQ
                     EFDADIYVNDKDKLELGELKMTFLHTPGHTQGCMCIRVEDEMFTGDTLFAGSIGRTDL
                     YSGNFNQMEKSLKKLCKYEDAVRVYPGHGPTSTLGVEKKTNPYM"
     gene            4400..5956
                     /locus_tag="KRL67_003032"
     CDS             4400..5956
                     /locus_tag="KRL67_003032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="coproporphyrinogen III oxidase"
                     /protein_id="HBH1523898.1"
                     /translation="MLYVFLKGHDYKYEVAELIKLFTSEFKFNDSKANNNMESKHLIN
                     RLIYENGVLFSSTEYYENGNLKYESIQNIENMNLEFLDGFDSSKTIEEFLNSLDNESK
                     RNFKKLCKENIKKSMFVVLKQVFNSYVPWGILTGIRPVKIVHNLMDKKLDDNSIRTIL
                     KDNYFIIDEKIDLALEIAKRERLFMYPIDKNKISLYVSIPFCPTRCVYCSFPSNSLKQ
                     FGHLKREYVYKLIEEIKGFAKVIKETKKEVETLYIGGGTPTTLDEEELDLLINSLFNE
                     LDLSKIKEFTVEAGRPDTITEQKLRVLKKHNVSRISINPQTMNDETLVKIGREHRVSD
                     LVDCFNMARRMGFDNINMDIILGLIDEDLNMVKNTLEEIKKLSPESLTVHTLAIKRAS
                     NLNINMDRYKEHLTQYEEMVKMIDLSMKYAKDMGLNPYYMYRQKHMLGNLENIGYAKE
                     GYECIYNIQIMEEKQSNYALGAGAISKFVYVDEDRIERVENVKNVEQYIERVDEMIKR
                     KKEEVYKNVD"
     gene            5946..7208
                     /locus_tag="KRL67_003033"
     CDS             5946..7208
                     /locus_tag="KRL67_003033"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HBH1523899.1"
                     /translation="MLTKAPRGTKDITPKEAYKWRYVENKFREICALYGYEEMVTPIF
                     EHTELFKRSVGDTTDIVQKEMYSFKDKGDREITLKPEGTAGVVRAFIENKLYADTQPT
                     KLFYVTPCFRYERPQAGRQRQFHQFGIEALGSDTPSMDAEIIALAVQFFNEVGLNDLV
                     VSINSVGCPVCRKEYNALLKEYLDSKADILCDTCNERREKNPMRVIDCKNPTCKENIK
                     DIPFIADHLCDDCKSHFDKLQEYLKEMNINFVIDKTIVRGLDYYRKTAFEIISNDIGA
                     QSTVCGGGRYDGLVEQLGGPKGISGIGFGLGIERLLLTLEGNGIEIENPQSTDIFIVT
                     IGEEANTRSFKLLKDLRQNHISADKDHIERSVKAQFKYSDKINSKFTIVIGDDELKND
                     TATLKNMKTSEQTTVKLSTLVEELKQKL"
     gene            7282..9069
                     /gene="aspS"
                     /locus_tag="KRL67_003034"
     CDS             7282..9069
                     /gene="aspS"
                     /locus_tag="KRL67_003034"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HBH1523900.1"
                     /translation="METLKGLKRTHYCGELREKNINEEVVLMGWVQKKRNLGGLVFVD
                     LRDTSGLCQIVFDTDVDKEAFEKAEKLGAEFVIAVKGKVYERQSKNPNMPTGDIEIFA
                     TELRLLNKSETPPIYIKDDDDVSEALRLKYRYLDLRKPSMQRNLKLRHKVMNITRNYL
                     SNNRFCEIETPFLIAPTPEGARDYLVPSRVNPGKFYALPQSPQLYKQLLMVSGMDRYF
                     QIVKCFRDEDLRADRQPEFTQIDCEMSFVEQEDVMSMIEGLLEAIFKEVLDVELTLPL
                     PKMTYAEAMSKYGSDKPDTRFGYELTDISDVVCNCGFKVFADATQPGKSVRGINVKGK
                     ADDFTRKQISSLEEHAKTYRAKGLAWMKVGQEGVTSPIAKFFNEEEMNAILTRMNAEV
                     GDLLLFVADKNSIVFDALGQVRLEVANRLNLLDKNVYNLLWVTEFPVFEEDEETGTFS
                     AMHHPFTSPMDEDLDKLEEGDKASLRAKAYDIVLNGYEIGGGSVRISNSDVQSRMFKA
                     LGFTEERANEKFGYLLEAFKYGTPPHAGLAFGLDRLVMLLAGTDNIREVIAFPKNQNA
                     VCPMTNAPTLAEDEQLEELSIKVDIKDNE"
BASE COUNT         3707 a          975 c         1777 g         2715 t
ORIGIN      
        1 taaaggcgaa tattcgtata tatatgagaa taaaagagaa gaaagtgaaa ttataaaata
       61 ttataataaa acagacctat tatatttaaa gaatgtagtt agtaatatag ttccatcaag
      121 agatgaattt ataacaattt ataaacaagc actcataaaa aaagaaattg atttggatat
      181 ggtaaatata aggggaactt ttaatgtaat tccattaaag ttttttacga tattaaatgt
      241 atttagagaa ttaaatttac ttgactttaa tttaaattat gaaaaaaatt ctgtactcat
      301 aagaatattg ccaaaaccac agaaaaaatt agacttaaat gagagtttaa tactcaataa
      361 tctaaaaaat ttagaaaaac aatacaatag cagttattag gaggcgtttc ccatggattt
      421 aaaaaacttt ataaggaata ttgatgattt cccaaagcca ggtatagatt ttaaagatgt
      481 gacaactcta tttaaagatg gagatgcatt taaatatgca gtggattcta tagttgaaga
      541 attaaaagat aaagatgtag atttagtaat aggaccagaa gcaagaggat ttttgatggg
      601 tacacctgta gcatatgctt taggagtagg atttgttcca attagaaaac ctggaaagtt
      661 gccaggtgaa gttgagagtt atgagtatgg attagagtat ggtacagata ctttagagat
      721 acataaggat gcaattaaaa agggtcaaaa agtagcgata gtagatgatt tattggctac
      781 tggtggaact atggaagctg ctgctaagct tgtagaaaaa ttaggtggag aagtagtttc
      841 tatgcaattt ttgattgagc taaaattctt aaatgggaga gaaaaattgt ctaactatga
      901 tgttaattct cttataaaat actagtactt ttataatagt tggtataaca ccaactattt
      961 tcatatatat taaaatttta tacaaaatag gtggttatat gcatgataaa gaattacaag
     1021 aaataattga aaaaataaaa gtttatgcac ctaatgcaaa ttttgaaatg gttgaaaaag
     1081 cctataatct tgcaaaaaaa gcgcatgaag gacaatatag aaaatctggg gaaccatact
     1141 ttattcatcc attagcagtt gcaaatattt taatagagat ggaattagat attgaaacta
     1201 taacagcggg tttacttcat gatgttgtag aagatacaga atatacctat gaagacataa
     1261 aaaaagaatt tggcaaagaa gtagcggatt tggttgatgg agtaactaaa cttggtcaaa
     1321 taaaatatcg ttcgaaagaa gaaactcaat ctgaaaattt aagaaaaatg tttttggcaa
     1381 tggcgaagga tataagggtt atacttataa aacttgctga tagattgcac aacatgagaa
     1441 cattaaaata catgcctcca gaaaaagcaa agtataaagc aaaagagaca cttgaaattt
     1501 atggtggtat agctcataga ttaggaattt ccaagataaa gtgggaacta gaagacagag
     1561 cattaagatt tatggaccca gaagggtatt atgatttagt tagtagagta tcaatgaaga
     1621 gaagtcaaag agaggattat attcaaggaa ttgttgaact tcttaaagat aagtttaaag
     1681 atttaagtat accatgtgat gtatatggta gaccaaagca tttttacagt atatatagaa
     1741 agatgcaaaa aaaacataag acttttgaag agatttttga tttaacagca gtaaggatat
     1801 tggttgataa tgtaaaggat tgctatgcag tacttggtat agttcataca ttatggagac
     1861 ctattccagg aagatttaaa gattatatag caatgccaaa accaaatatg tatcaatcat
     1921 tacacaccac tgttgtaggt cctgatggag aacctttaga gatacaaata agaactcatg
     1981 agatgcataa tatagctgaa aatggtatag cagcccattg gaaatataaa gaaggtatct
     2041 ctagcaaaga gtcaaaggtt gaagaaaaac ttcaatggct gagacagatg atggagtggg
     2101 aaaaagattt aaaagaccca caagagttta tggatgcact taaagaagat gtatttaata
     2161 gtcaagttta tgtatttaca ccaaaaggag acgtaattga attaccagca gaatcaacac
     2221 ctatagattt tgcctatagg gttcatacaa atgtaggtaa taaatgtgta ggagctaaaa
     2281 ttgatggaag aatagttcct attgattata agcttcaaaa tggtaatatt gtcgaggtgc
     2341 ttacatcatc taattctaat ggaccaagta gggattggct taatatagtg aagaccccaa
     2401 atgcaaaaag tagaattaga caatttttca agaaggaaag aagagaagaa aatgttgacc
     2461 gtggaaatac aatattagaa agagaattta agaagcatga tataccacaa aaagaccctc
     2521 ttgttgaaaa atatatggca ttagttgcta agaaatttaa tcagccaagt gtggaggact
     2581 taattgccac tgtaggttat ggtggtataa tgtcatctca agtggtatct aaagtaaaag
     2641 acctatattt aaaagaggtt aaaaaagcta aaaaggaaca acagatacaa gaagaagatt
     2701 taaataaaca taatataagt gaaacagaat acagtaaaaa gagaaagaaa aatgcatcac
     2761 aaggaattat agtaaaaggg cttgacaata tcttagtaag atttgctaag tgttgtaatc
     2821 ctcttccagg agatgaaatt gtaggatata ttactaaggg taggggagta gccatacaca
     2881 gagctgattg cccgaatgca aacaataatg gtgatttctt ccaaaataga ttggtagagg
     2941 tttcatggag ttcagcagga aaaggtaagt ttgaagcgga aattcaaata aaagctattg
     3001 acagaagagg aattgttaat gaaataactc atattgttgc aatggataaa cttagtttaa
     3061 atggaataaa tgcaagaaaa ggtaaggata gtattgtaag tatcaactta cttgtagaag
     3121 ttaatgatac agaggaatta aaaattctaa tgaaaaaatt aaaagcaatt cctggtgtag
     3181 aaagtattta tagaatgaca aattagagtt aaaataatag ggaggaagct atgagagcag
     3241 tagtacaaag agtatcttca tctaaagtta cagtagatga aaacactata ggtcaaataa
     3301 ataaggggtt actagtattg ttaggagtga ctcatgatga taaatcaagt gatgtagatt
     3361 atatgattga caaaatattg aatctgagaa tttttgaaga tgaaaatgac aagatgaatt
     3421 tatctctaat ggatataggt ggagaacttt tagtagtatc tcaatttact ttatatggag
     3481 attgtagaaa aggaagaaga cctggattta gtaatgcagc tagaccagaa cttgcaaata
     3541 acctttatga agaatttgta aaaaaagcaa aggataaagg tgtgacagtt ggcacaggtc
     3601 aatttgcagc tcatatgatg gtagagttaa ctaatgatgg tccagtaaca atactattag
     3661 actcaagtaa atctttttaa gggggaaaac tttaatgatt ataaaaaaat ttgtagatag
     3721 ttattttgga gtaaatactt atgtattagg tgatgaaaag actaaaaaat gtgcagttat
     3781 agacccaggt ggaagtttag tagagacatt aagttttgta aaagaaaatg aattaaatat
     3841 tgaatatatt atccttactc atgggcatgg agaccatata ggatatgtta aggatattaa
     3901 agaaaaaact ggtgctaagg tagttgcaca tattgctgaa aaagaattat taaatgacaa
     3961 gaataaaaat ctaagttata caatgagatg tggagcgcaa gagtttgatg cagacatcta
     4021 tgtgaatgat aaagacaagc ttgaacttgg tgaattaaaa atgactttct tgcatactcc
     4081 aggacatact caaggatgta tgtgtataag agttgaagat gaaatgttca caggagatac
     4141 tttatttgct ggaagtattg gtagaactga tttatatagt gggaatttta accaaatgga
     4201 aaaatcactt aagaaattat gtaaatatga agatgctgtt agagtatatc ctggacatgg
     4261 accaactagt actctagggg ttgaaaagaa gacaaatcct tacatgtaat tttatattag
     4321 tatttaaaaa agtataaaat atttaataac tatattagtg agggggttct tctactgtta
     4381 aatagaagga gttaagttga tgttgtatgt ttttttaaaa ggacatgatt ataagtatga
     4441 agtagcagaa cttataaaat tgtttaccag tgaatttaag tttaatgact ctaaagctaa
     4501 taataatatg gagtcaaagc atttaataaa tagattgatt tatgagaatg gtgtattatt
     4561 ctcaagtact gaatactatg aaaatggaaa cttaaagtac gagtcaatac aaaatataga
     4621 aaatatgaat cttgaatttt tagatggatt tgatagtagc aaaacgattg aagaatttct
     4681 taatagctta gataatgaaa gtaagaggaa ctttaaaaag ctgtgtaaag aaaatatcaa
     4741 aaaaagcatg tttgtagtgc ttaaacaagt atttaattct tatgtaccat ggggaatttt
     4801 gactggtatt agaccagtca aaattgtcca caatttaatg gataaaaagc tagatgataa
     4861 tagcataaga accattctaa aagataatta ttttataata gatgagaaaa tagatttagc
     4921 tcttgaaata gctaaaagag agcgactatt tatgtatcct atagataaaa ataaaatttc
     4981 tctttatgta agtattccat tttgtccaac tagatgtgta tattgttcat ttccttcaaa
     5041 ttcattaaaa caatttggac atttaaaaag agaatatgtt tataaattga ttgaagaaat
     5101 taaggggttt gccaaggtta ttaaggagac taagaaagaa gttgaaactc tatatattgg
     5161 tggtggaact ccaactacac ttgatgagga agaattggat ttgcttataa attcattatt
     5221 taatgaatta gatttaagta aaattaaaga atttactgta gaagcaggta gacctgacac
     5281 tattacagaa caaaaactaa gagtgttaaa aaaacacaat gttagtagaa ttagtataaa
     5341 tcctcaaact atgaatgatg agaccttagt aaagattgga agagagcata gagtatctga
     5401 tttagtagat tgctttaata tggctcgtag aatgggattt gataatataa atatggatat
     5461 aattttaggt cttatagatg aagacttaaa tatggtaaag aacacattgg aggagataaa
     5521 aaaactttct cctgaaagcc ttacagttca tacattagcc ataaagagag catccaactt
     5581 aaatataaat atggataggt ataaagaaca tctaacacaa tacgaagaaa tggtaaaaat
     5641 gatagattta tcaatgaaat atgcaaaaga tatgggatta aatccatatt atatgtacag
     5701 acaaaaacat atgttgggaa atttagaaaa tataggttat gctaaagaag gatatgagtg
     5761 catttataat atacagatta tggaagaaaa acaaagtaat tatgcattag gtgcaggtgc
     5821 tatatcgaaa tttgtttatg tagatgaaga tagaattgaa agagtagaaa atgtaaaaaa
     5881 tgttgaacaa tacattgaaa gagtagatga aatgataaaa aggaagaaag aagaggtata
     5941 taaaaatgtt gactaaagct cctagaggaa ctaaagacat aactccaaaa gaggcttaca
     6001 aatggcgtta tgtagaaaat aaatttagag aaatatgtgc tttatatgga tatgaagaaa
     6061 tggtaacacc tatatttgaa catacagaac ttttcaagag aagtgttgga gatactacag
     6121 atatagttca aaaagaaatg tattctttta aagataaagg tgatagagaa attaccctaa
     6181 agccagaagg tacagctggt gtagtaagag catttataga aaataaatta tatgcagata
     6241 ctcaaccaac aaaattattt tatgtgacac cttgttttag atatgaaaga ccacaagctg
     6301 gtagacaaag acaatttcac caatttggaa tagaagcact tggaagtgat actccttcaa
     6361 tggatgctga aataatcgca ctagctgttc aattttttaa tgaagttgga ttaaatgatt
     6421 tggtagtgag tataaattca gttggttgtc cagtttgcag aaaagagtac aatgctttat
     6481 taaaagaata tcttgactca aaagctgata tattgtgtga tacttgcaat gagagaagag
     6541 aaaaaaatcc aatgagggtt atagactgta aaaatcctac atgtaaagaa aatatcaaag
     6601 atataccatt tattgcagac catttatgtg atgattgtaa atctcacttt gataaacttc
     6661 aagaatactt aaaggaaatg aatataaact ttgtaattga taaaacaata gttagaggct
     6721 tagattacta tagaaaaact gcttttgaaa ttatatctaa tgatatagga gctcaaagta
     6781 ctgtatgtgg tggaggaaga tatgatggtc ttgttgaaca actaggaggg ccaaaaggta
     6841 taagtggaat tgggtttgga ctaggaattg aaagactttt acttacactt gaaggtaatg
     6901 gaattgaaat agaaaaccct caatctacgg atatatttat agtgactata ggtgaagaag
     6961 caaatacaag aagctttaaa ttgttaaaag acttgagaca aaatcatata agtgcagata
     7021 aagaccatat agagagaagt gttaaagcac aatttaaata ttcagataaa ataaattcaa
     7081 aatttacaat agtaattgga gacgatgaac ttaaaaatga cactgcgaca ttaaaaaata
     7141 tgaaaacatc agagcaaact actgtaaaat taagtacatt agttgaagaa ttaaagcaaa
     7201 agctgtaaaa taagaaagta tgatgttttg tgtattagat aaatatatag cattaaaatt
     7261 aaataaaatg gaggtagtat cttggaaact ttaaagggtt taaagagaac tcactactgt
     7321 ggagagttga gagaaaaaaa cataaatgaa gaagttgtac ttatgggatg ggtacagaaa
     7381 aaaagaaatt taggtggtct tgtatttgtt gatttaagag atacaagtgg attatgtcaa
     7441 atagtttttg atacagatgt agataaagaa gcttttgaaa aagcggaaaa attaggggct
     7501 gagtttgtaa tagctgtaaa aggaaaagta tacgaaagac aatctaaaaa cccgaatatg
     7561 ccaactggtg atatagaaat atttgcaact gaacttagac ttttaaacaa gtcagaaact
     7621 ccgcctattt atataaaaga tgatgatgat gtttctgaag cactaagatt aaaatataga
     7681 tacttagatt taagaaaacc atctatgcaa agaaatttaa aattaagaca taaagtaatg
     7741 aatattacaa gaaattactt atcaaataat agattttgcg aaatagaaac accattttta
     7801 atagctccaa caccagaagg agcaagagat tatctagtac caagtagagt taatccagga
     7861 aaattttatg cattaccaca atcaccacaa ttatataagc aattattgat ggtaagtggc
     7921 atggatagat atttccaaat agttaaatgc tttagagatg aagacttaag agcagataga
     7981 caaccagagt ttacacaaat agactgtgaa atgtcttttg ttgagcaaga agatgttatg
     8041 agtatgattg aaggtttgct tgaagcgata tttaaagaag ttttagatgt agaattgaca
     8101 cttccacttc ctaagatgac ttatgcagaa gctatgtcta aatatggttc agataaacca
     8161 gatactagat ttggatatga gttaacagat atatctgatg ttgtttgtaa ctgtggattt
     8221 aaagtgtttg ctgatgccac acaaccagga aaaagtgtta gaggtataaa cgtaaaaggc
     8281 aaagctgacg actttactag aaaacaaata tcatcactag aagaacatgc taagacatat
     8341 agagcaaaag gtcttgcttg gatgaaagta ggacaagaag gagtaacttc tccaatagca
     8401 aaattcttta atgaagaaga aatgaacgct atacttacaa gaatgaatgc tgaagtagga
     8461 gatttacttt tatttgtagc tgataaaaac tcaatagtat ttgatgcttt aggacaagtt
     8521 agacttgaag tagcaaatag gcttaatcta ttggataaaa atgtatataa cttactatgg
     8581 gtaactgagt tcccagtttt tgaagaagat gaagaaacag gcacattctc agcaatgcat
     8641 cacccattta catcacctat ggatgaagat ttagacaagt tagaagaagg tgataaggca
     8701 tctttaagag cgaaagctta tgatattgta ttgaatggtt atgaaatagg tgggggaagt
     8761 gttagaatat caaattctga tgttcaatca agaatgttta aagcacttgg tttcacagag
     8821 gaaagagcta atgaaaagtt tggttattta ttagaagcat ttaaatatgg tactcctcct
     8881 catgctggtc tagcttttgg tcttgataga cttgttatgt tacttgcagg aactgataat
     8941 ataagagaag ttattgcatt ccctaaaaat caaaatgctg tttgccctat gacaaatgct
     9001 cctacattgg ctgaagatga gcaattagaa gaattaagca taaaagttga tataaaagat
     9061 aatgaataat atgtagttgc aagaaactaa aatgtaaaaa gtctggatat aaagtatatt
     9121 tatattcaga ctttttttta ttagaattat tttatatatt ctctctatat attt
//
LOCUS       DAEPXF010000156         9132 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain HB27
            SAMN13376389-rid12479423.denovo.163, whole genome shotgun sequence.
ACCESSION   DAEPXF010000156 DAEPXF010000000
VERSION     DAEPXF010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13376389
            Sequence Read Archive: SRR10518432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 9132)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9132)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001069624.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001069624.1
            Genome Coverage       :: 315x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/15/2021 01:54:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,165
            CDSs (total)                      :: 4,076
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 89
            rRNAs                             :: 10, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 10 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 19 of 69
            Pseudo Genes (incomplete)         :: 44 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 10 of 69
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9132
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13376389-rid12479423.denovo.163"
                     /strain="HB27"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="China: Hebei"
                     /collection_date="2017"
     gene            complement(39..230)
                     /locus_tag="KRL67_003035"
     CDS             complement(39..230)
                     /locus_tag="KRL67_003035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1450 domain-containing protein"
                     /protein_id="HBH1523901.1"
                     /translation="MIRVCPNCSNVDVNKLKTLVGEENVKTGCIGQCRAFKKEAVGII
                     DNELEIKETEAEFFEACKE"
     gene            complement(378..620)
                     /locus_tag="KRL67_003036"
     CDS             complement(378..620)
                     /locus_tag="KRL67_003036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH1523902.1"
                     /translation="MLEVTKIIIKYFASESDTFVKEEVCNFEELLKWSHKFIEGKKIE
                     DVLYIKSKENYKVNVIKSLEIKELEIVRKQQIPPYM"
     gene            832..2655
                     /gene="typA"
                     /locus_tag="KRL67_003037"
     CDS             832..2655
                     /gene="typA"
                     /locus_tag="KRL67_003037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HBH1523903.1"
                     /translation="MSQKHKIINIAVIAHVDAGKSTLVDAFLSQSGVFRKNEVVKDCV
                     MDSNDLEKERGITIYSKNCAINYEDYKINIVDTPGHSDFSSEVERVMKTVDTVILLVD
                     ASEGPMPQTRFVLQKSLEFGLKPILFINKIDKKDQRAEEVVNEVFDLFVDLNATDEQC
                     EFPIIYGIAKQGIAKLEMDDDSEDLSPLFKTIVNHVEAYPNYDNEPLQFQISALAYDD
                     YVGRLGIGRIYKGTLKNNTQVAICREDSVVSKGKVSKLSVYEGLKQVEVDEATSGEIV
                     VIAGIPDISIGETICDLDSPLPMEMIKIEEPTLSMNFLVNDSPFVGKSGKFVTTRHLK
                     DRLEKELEVNVGLKVEPLDTTDGYKVSGRGELHLSILLENMRREGYEVGVSKPEVLMH
                     KEDGKLMEPIERVVVNCPEVYSGTIINELNMRKGMMESMSIEGDYVKIEFLAPTRGLL
                     GYRSEFINATRGEGTLVRSFEKFEEFKGEIPSRGNGVLIAQGPGVTMGYSLNALSDRA
                     VMFVDPGVEVYEGMIIGMNSRKDDMVVNPCKNKKMSNVRASGSDDAIKLSPPRIFTLE
                     EALEFIEDDELVEITPDSIRLRKRFLNEHDRLRYNKSRQGK"
     gene            complement(2748..3656)
                     /gene="trxB"
                     /locus_tag="KRL67_003038"
     CDS             complement(2748..3656)
                     /gene="trxB"
                     /locus_tag="KRL67_003038"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HBH1523904.1"
                     /translation="MVDIIVIGAGPAGLTSAIYAMRAGLSVTVFEKNIYGGQVASTSE
                     VENYPAVQKISGVEFSNNIYNQAVAQGVDIQFDEVEEINLEEKVKVVKTSSGEHKAKA
                     VILANGVERRKLGCAGEQEFTGRGVSYCATCDGAFFKDKEVAIVGGGNTALEDALFLA
                     NNCTKVYLIHRRDSFRGEEVLEKSVKARKNIEILYSHGVEKIEGEKTVSKIEVKNLKT
                     EEKRTIDVSGIFIAIGLKPNNKMFENVLDLDEGGYIISDESCTTSVEGVYVAGDSRTK
                     FLRQIITAASDGAIAAVQAANYINVE"
     misc_binding    4458..4689
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            4797..6278
                     /locus_tag="KRL67_003039"
     CDS             4797..6278
                     /locus_tag="KRL67_003039"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HBH1523905.1"
                     /translation="MYYSSTRGTEEKVTASQAIIKGISSDGGLYVPSEFPNVKNELIN
                     LVNLTYSQIAFFVLSKFLCDFTEDEIKNCIENAYDEKFDCSSIAPLNKVNDTYFLELY
                     HGPTLAFKDMALTIMPHLLKTSIKKDNLEKDVVILTATSGDTGKAALEGFKDIDKIKI
                     IVFFPEDGVSPVQKLQMKTQTGKNTYVVGIKGNFDDAQSGVKKIFSDKEFNQKLLDNN
                     YILSSANSINIGRLLPQVAYYFYSYMKLVSNSKINLNDKINFVVPTGNFGNILAGYYA
                     KQMGLPINKLICASNDNNVLYDFFKTGVYDKNRTLKLTTSPSMDILISSNLERLLFEI
                     SNRNTDIVNKLLSDLSDKGVYKISDDMEKNLASFHGEYSNEEEVKSTINNVFKNYNYL
                     IDTHTAVAYNCYEKYKKETSDNTKTVIVSTASPFKFSEDVLKSIDCDFKDLDDFAIID
                     RLSSISKVDIPKPIKNLKNAEILHKDIYEKDELKLAIKKFLKV"
     gene            6288..7181
                     /locus_tag="KRL67_003040"
     CDS             6288..7181
                     /locus_tag="KRL67_003040"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HBH1523906.1"
                     /translation="MLEIIVPATSANIGPGFDCLGIALNIYNKFYVEEIESGLEIEGC
                     EDAYKNENNLVYTSMKYFFDRVKPEKIPAGIKIKIQSEVPICRGLGSSASCIVAGVIA
                     ANALSGANLDKNQLLNIASEIEGHPDNVAPAILGNMIVSVTDNENIHYDIIKIPEELK
                     FCAMIPNFKLSTEKARGVLPKEIPYSAGVFNVSRVALLVSALLNKNFDLLKVACQDKL
                     HQDYRGTLIENYNDIVEKSEQLNSIGVFLSGAGPTIMSLIKENDDSFVDNMKNYLQKL
                     KSDWEIKELCCDSNGAVLNII"
     gene            7305..8366
                     /locus_tag="KRL67_003041"
     CDS             7305..8366
                     /locus_tag="KRL67_003041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015617764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HBH1523907.1"
                     /translation="MIIKELIIRDLVAKVPIIQGGMGVGISRSSLAGAVAKLGGVGVI
                     SGVQIGYDEEDFETNTINANLRAIKKHISKAKEISNGGIIGINFMVAMKEYETYVKEA
                     VKAGVDLIISGAGLPNKLPSLVKGSNVKIAPIVSTAKAANVILKMWDRKEKTTADLIV
                     VEGPKAGGHLGYSNEELDNIDSIDYDKEFVEILKVANTYGEKFGRNIPVVAAGGITSS
                     SDVKKYIDMGASGVQVGTRFVATYECDAHENFKMAYINASEEDVHIVKSPVGLPGRAI
                     RNKFIEEVKINRPEIKKCYNCLIPCNPKETPYCISQALINAVKGDVENSLLFCGNDAY
                     KIDKLSTVEDVINELISEL"
     gene            complement(8508..9020)
                     /locus_tag="KRL67_003042"
     CDS             complement(8508..9020)
                     /locus_tag="KRL67_003042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HBH1523908.1"
                     /translation="MSYFKEILAEEIKNNPFGLIGKDWTLITAENEGKVNTMTASWGG
                     LGVMWGKDVAFVVIRPQRYTKEFIDNTDKFSLTFFDEDFRKELSYCGKVSGREEDKIS
                     QIGFNIEHLNDTPYFKEAKMAIICKKMYSQKLEPQCFIAEGIDGRWYPQKDYHTLYIA
                     EITNVLIKED"
BASE COUNT         3262 a         1280 c         1318 g         3272 t
ORIGIN      
        1 tatgctctat ttattataat ttatatttta tttaaaattt attctttgca tgcttcaaaa
       61 aactctgctt ctgtttcctt tatttcaagc tcattgtcta ttattccaac agcttctttt
      121 ttaaatgctc tacactgacc tatacaacca gtctttacat tttcttctcc aaccaaagtt
      181 tttagcttat ttacatccac atttgaacag tttggacata ctcttatcat atttaagttc
      241 ctcctaatta atgtctaatt aatacgttta tacccaaata taaactttat aaaagttttt
      301 ttaatataaa ttattaccat atataccatt tatatcctat tgtgattatt acatattaat
      361 tttatatatt attaatacta catgtatggt ggtatctgtt gttttctcac tatttccaac
      421 tccttaattt ctaaagactt aatcacattt actttatagt tttctttaga ctttatatac
      481 aaaacatctt ctattttttt accttcaata aatttatgag accacttaag aagttcttca
      541 aaattacaaa cttcttcttt tacaaaagta tcactttcac ttgcaaaata tttaattatt
      601 attttggtta cttccaacat atatcatcct ttactctttt attaatcttt ttatctatta
      661 aggttattat ttgtctaaaa aattttttta aacagaataa ttttcagatg taaaactaac
      721 agttttatcc aatttaaact atattaatac aatttaatat attcctcctt gtacctaata
      781 atatattgtg ctataattaa taagttattt taatcgaagg gagcaacaaa aatgtcacaa
      841 aaacataaaa ttataaatat cgcagttata gcccacgttg acgctggaaa atcaacattg
      901 gttgatgctt tcctttctca aagtggtgtt tttagaaaga acgaagtagt taaagactgc
      961 gttatggaca gtaacgacct tgaaaaagaa agaggcataa ctatatattc aaaaaactgt
     1021 gcaataaact atgaagatta caaaataaac atagttgata ctccaggaca cagtgacttt
     1081 tcttctgaag ttgaacgtgt tatgaaaact gttgacacag ttattctatt agttgatgct
     1141 agtgaaggcc caatgcctca aactagattc gttttacaaa aatctttaga gttcggttta
     1201 aaaccaatat tatttataaa caagattgat aaaaaagacc agagagcgga agaagtagtt
     1261 aatgaagttt tcgatttatt tgtagactta aatgcaacag atgagcaatg cgaatttcct
     1321 ataatatatg gtattgctaa acaaggtatt gctaaacttg aaatggatga tgatagtgag
     1381 gacctttctc ctctatttaa aactattgta aatcacgttg aagcttatcc taattacgat
     1441 aatgaacctc tacaattcca aatatcagct cttgcatatg atgattatgt aggtagactt
     1501 ggtataggta gaatttacaa aggtactctt aaaaacaata cacaagttgc tatatgtaga
     1561 gaagattctg ttgtatctaa aggaaaagta tcaaaacttt ctgtgtatga aggtttaaaa
     1621 caagttgaag ttgatgaagc tactagtgga gaaatagttg ttatagctgg tattcctgat
     1681 atatctatag gtgagactat atgcgattta gatagtccac tacctatgga aatgataaaa
     1741 atagaagagc ctactctttc tatgaacttc ttagtaaatg actctccatt cgttggaaaa
     1801 agtggaaaat tcgttacaac gagacatttg aaagatagac ttgaaaagga acttgaagta
     1861 aatgttggtc ttaaagttga acctcttgat actactgatg gatacaaagt ttctggacgt
     1921 ggagagcttc acttatctat actacttgaa aatatgagac gtgaaggtta tgaagttggt
     1981 gtttctaaac ctgaagtttt aatgcataaa gaagatggaa aactaatgga accaatagaa
     2041 agagttgttg taaactgtcc tgaagtttac tccggtacta ttataaatga attaaacatg
     2101 agaaaaggta tgatggagtc tatgtcaata gaaggagact atgtaaaaat agagttctta
     2161 gcacctactc gtggtctttt aggatatcga agtgagttta taaacgctac tcgtggagaa
     2221 ggtactttag ttcgttcatt tgaaaaattt gaagaattta aaggagaaat accttcaaga
     2281 ggaaatggtg ttttaatagc acaaggacct ggtgttacta tggggtattc tcttaatgcc
     2341 ttaagtgata gagctgtaat gtttgtagac cctggtgtag aagtttacga aggtatgata
     2401 attggtatga attccagaaa agatgatatg gtagtcaacc cttgtaaaaa caagaaaatg
     2461 agtaacgtac gtgcttctgg ttcagatgat gctataaaat tatctcctcc tagaatattt
     2521 actcttgaag aagcactaga atttatagaa gatgatgaat tagttgaaat tacacctgat
     2581 tctataagac ttagaaaaag attcttaaat gaacatgata gattaagata taacaaatct
     2641 agacaaggta aataaaacat acacttacct ctaattagca aaatagccat atagtactga
     2701 aaaattattt cagtttatat ggctattttt atttgaaatt ttttatacta ttcaacattt
     2761 atataattag ctgcttgaac ggctgcaata gctccatctg atgctgctgt tattatttga
     2821 cgtaaaaact tagttctact atctcctgct acatatacac cttctactga tgttgtacaa
     2881 gactcatctg aaattatata tccaccttca tccaaatcaa gaacattttc aaacatttta
     2941 ttattaggtt taagacctat agcaataaaa ataccactaa catctatagt tcttttttct
     3001 tcagtcttta agttttttac ttctattttc gatacagtct tttctccttc tattttttct
     3061 acaccatgac tgtaaagtat ctcaatattc tttcttgctt tcacagattt ctccaacact
     3121 tcttcacctc taaaactatc tcttctgtga attaaataca cttttgtgca attattagct
     3181 aaaaataaag catcttctag tgcagtattt cctccaccta ctatagcaac ttctttatct
     3241 ttaaaaaatg ctccatcaca tgttgcacag taagatactc ctctacctgt aaattcttgt
     3301 tcccctgcac aacctaattt acgtctttca actccatttg caagaataac agcctttgct
     3361 ttatgttcac ctgaagaagt ttttacaacc tttacttttt cctctaaatt tatctcttca
     3421 acctcatcaa actgaatatc aacaccttga gcaactgctt gattgtatat attattagaa
     3481 aattctaccc cagaaatctt ttgaactgct ggataatttt ctacctcaga ggtacttgca
     3541 acttgacctc cataaatatt tttttcaaat actgtaacgc ttagtcctgc tctcatagca
     3601 taaattgctg aagttaaacc tgctggtcca gcaccaatta caatgatatc taccataacg
     3661 tttacctctc tttatattat tatttcttat atattattct cattattctt atactttact
     3721 attattatcc atatatacta taaacaaaac attatttttt attaattaac tttattttac
     3781 atatatataa aaaaactgca aataaaaatt aacttttatt tacagttctc acctatttat
     3841 ataattcaaa accttattgg ttatcatttg cagtgtcttc taatatattg ctttctgata
     3901 tatatgatag aattgaaagt ccatacataa ccaaaactaa tgctgctgtt ataagtacta
     3961 ttcccatatt taatacctcc ttttgtttac agtatactat tattatgtga aatatttatg
     4021 aatttaattt gaatatatat gtaatttcac actaacaatt tcttcaaatt aaacattatt
     4081 aactatatac ttttgttgaa ccaattatga ttaatagttt aaatattagc actatttact
     4141 tatatttata tgatatacca tataattaca tttttcaaat taaagttttg taaacttttt
     4201 ttgtaatata gtgtagtata aaattgtaat attacagata tttaatggtc atcttttgat
     4261 tactttcatt aaaaacacct tcatcactat gaaaatctag gtttttaagc ttttaaaaaa
     4321 gttaaaaaaa tatttttcta atactcaatt tgattacaat ttttttacaa tttaaaaaat
     4381 attttttcca aatttttata ttaatacttg actttgtctg ttttataaac tagaataatt
     4441 aataatatta agaatattga aaggcattga ttaggaagag taaatctgaa tcgattacca
     4501 gagaaggaaa ctactagctg aaatgtttcc ctaattagaa ggattgaaaa ctacctagga
     4561 gcttctatcc gaagttatct taatgatttt agtaggaata gacgttttta ttacttgcgt
     4621 taaagtatca agtggatttt ttaaaatcaa tttgggtgga accgcgggaa acagattctc
     4681 gtcccttttt tagggatgag ggtctttttt tattacctaa atttaatttt taaaatattc
     4741 tgtatctaac ttaattagct aattaaaaat ttaaaatata tggaggatta taaaaaatgt
     4801 attattcaag tacgagaggc accgaagaaa aagtaactgc atcacaagca ataataaaag
     4861 gaatatcaag tgatggtggt ctttatgttc caagtgaatt tcccaatgta aaaaatgaac
     4921 ttataaattt agtaaattta acgtattctc agatagcatt ttttgtactt agtaaatttt
     4981 tatgtgattt tactgaagat gaaattaaaa actgtataga aaatgcatat gatgaaaagt
     5041 ttgattgtag tagtatagct ccattaaata aagtaaatga tacttatttc ttagaactat
     5101 atcatggtcc aactttagca tttaaagata tggcacttac tataatgcct catcttctta
     5161 aaacatctat aaagaaagat aatttagaga aagatgttgt gattcttact gctacatctg
     5221 gagatacagg caaagctgca cttgaaggat ttaaagatat agataaaata aagataattg
     5281 tattcttccc agaagatggt gtaagcccag tacaaaaact tcaaatgaaa actcaaactg
     5341 gaaaaaatac atatgtagtt ggtataaaag gaaattttga tgatgcacaa tcaggtgtaa
     5401 agaagatttt ttctgataaa gaatttaatc aaaaactatt ggataataat tatatattat
     5461 cttctgcaaa ttcaattaat ataggaagac ttcttccaca agttgcatac tatttttatt
     5521 catatatgaa attagttagc aatagtaaga taaatttaaa tgataaaata aattttgttg
     5581 tcccaactgg taattttggt aatattctag ctggatatta tgccaaacaa atgggtcttc
     5641 caataaacaa attgatatgt gcatcaaatg ataataatgt tctttatgat ttctttaaaa
     5701 ctggtgtata tgataaaaat agaacattga aattaactac ttctccttct atggatatat
     5761 taatttcaag taatttagaa cgattactgt ttgaaatatc aaatagaaat actgatattg
     5821 taaataagct gttatcagac cttagtgata aaggagtcta caaaataagt gatgatatgg
     5881 aaaaaaattt agcctcattc catggagaat attcaaatga agaagaagtt aaaagcacga
     5941 taaacaatgt gtttaaaaat tataattatt tgatagatac tcatactgct gttgcataca
     6001 attgttatga aaaatataaa aaagaaactt ctgataacac aaaaactgtt attgtaagta
     6061 ctgcaagtcc attcaagttt tctgaagatg ttctaaagtc tatagattgt gattttaaag
     6121 accttgatga ctttgctata attgatagat tatcaagtat ttctaaagta gatattccaa
     6181 aacctattaa aaatcttaaa aatgcagaaa tactacataa agatatttat gaaaaagatg
     6241 aattaaaact agctataaaa aaattcttaa aggtataggt gattattatg ttagagatta
     6301 tagttcctgc aacaagtgct aatataggtc caggatttga ttgtcttgga attgcgttaa
     6361 atatatacaa taaattttat gttgaagaaa ttgaaagtgg tcttgaaata gaaggttgtg
     6421 aagatgctta caaaaacgaa aataacttag tttatacatc tatgaagtac ttttttgata
     6481 gagttaaacc agaaaaaata cctgctggta tcaaaataaa aatccaaagt gaagtaccta
     6541 tatgtagagg tcttggcagt agtgcctctt gtatagtagc tggagttata gctgcaaatg
     6601 ctctatcagg tgctaattta gataaaaatc aattactaaa tatagcttca gaaatagaag
     6661 gtcacccaga taatgttgct cctgctattt taggaaatat gattgtatct gttactgata
     6721 atgagaatat acattatgat attataaaaa ttcctgaaga acttaaattt tgtgcaatga
     6781 tacctaattt taagttatca acagaaaaag ctagaggtgt tttaccaaag gaaataccat
     6841 attctgctgg ggtattcaat gttagtagag ttgctttact agtatctgcc ttactaaaca
     6901 aaaactttga cctacttaaa gtagcttgtc aagacaaatt gcatcaagat tataggggaa
     6961 ctctaattga gaactacaat gatattgttg aaaagtctga acaactaaat agtattggag
     7021 tatttttaag tggagctgga cctactataa tgtctttaat aaaagaaaat gatgatagtt
     7081 ttgtagataa tatgaaaaat tacctacaaa aactaaaatc tgattgggaa ataaaagagt
     7141 tatgttgcga ttctaatgga gctgttttaa atataatata attatcgtct tctatacaaa
     7201 atataactac tcttttatta taatttgtag tataataatt aggactaggt aataatatta
     7261 agtattataa tctagttctt taaacatgtt tacattgagg agggattatc ataaaagagt
     7321 taataattag agatttagtt gcaaaagttc ctataattca aggtggaatg ggcgttggta
     7381 tatctaggtc atcactagct ggtgcagttg ctaagcttgg tggtgttggt gtaatttctg
     7441 gagttcaaat tggatatgat gaggaagatt ttgaaactaa tactataaat gcaaatttaa
     7501 gagctataaa aaaacatatt tctaaggcca aagaaatctc aaatggtggt attattggta
     7561 ttaattttat ggtagctatg aaagaatatg aaacatacgt aaaggaagct gttaaagctg
     7621 gtgtagacct tataatatct ggagctggat tacctaataa gctaccatca ttagtaaagg
     7681 gaagtaatgt aaaaatagca cccatagttt caactgcaaa agctgctaat gtaattttaa
     7741 aaatgtggga tagaaaagag aaaactactg ctgacttaat agttgttgaa ggtccaaaag
     7801 ctggtggtca ccttggatac tctaacgagg aacttgataa catagattct atagactatg
     7861 ataaggaatt tgttgaaatt ttaaaagttg caaatactta tggagaaaaa tttggtagaa
     7921 acataccagt agttgctgct ggtggtataa cttctagtag tgatgttaaa aaatatatag
     7981 atatgggtgc aagtggtgta caagttggta caagatttgt tgctacttac gaatgtgatg
     8041 cccatgagaa ctttaaaatg gcatacataa atgctagtga agaagatgtt catatagtta
     8101 aaagtcctgt aggtcttcct ggacgtgcca taagaaacaa atttatagaa gaagttaaaa
     8161 taaatcgtcc agaaataaag aagtgctata attgtttgat tccttgtaat ccaaaagaaa
     8221 ctccttactg tatttctcaa gctctaatca atgcagttaa aggagatgtt gaaaattcac
     8281 ttttattttg tggaaatgat gcctataaaa ttgataaatt aagcactgtt gaagatgtta
     8341 tcaatgaatt gatttctgaa ttataatttt aaaaatttta tcaagctaag gagtttaaat
     8401 acgttattca ttaatcttta taaacatatt tacgtcctta gcttttttgt gtcaatttcc
     8461 ttttatggag attttaattt aattatagat agtattaacc tttatagcta atcttctttt
     8521 ataagtacat tggttatttc agctatatat aaagtatgat aatctttttg tggataccat
     8581 cttccatcta taccttcagc aataaaacat tgaggttcaa gtttctgact atacattttt
     8641 ttgcatataa tagccatttt agcttcttta aaataaggtg tatcatttaa gtgttctata
     8701 ttaaatccaa tctgtgatat cttatcttct tctctaccag atacttttcc acaatatgaa
     8761 agctctttac gaaaatcttc atcaaaaaat gtaagagaaa atttatctgt attatctata
     8821 aattccttag tataacgctg tggtcttatt actacaaaag caacatcttt tccccacata
     8881 actccaagcc ctccccagct agcagtcatt gtattcactt taccttcatt ttctgctgtt
     8941 ataagtgtcc agtctttacc tatcaatcca aatggattat ttttaatctc ttctgccaaa
     9001 atctctttaa aataactcat atctctaata acctctcatt ctcttatatt tatattcctt
     9061 ttattttcat gataaattat aaatgaagtc tagttttgaa aataaaataa tttttttaaa
     9121 tatattatta ca
//
LOCUS       DAEPXF010000157         9111 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain HB27
            SAMN13376389-rid12479423.denovo.164, whole genome shotgun sequence.
ACCESSION   DAEPXF010000157 DAEPXF010000000
VERSION     DAEPXF010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13376389
            Sequence Read Archive: SRR10518432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 9111)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9111)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001069624.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001069624.1
            Genome Coverage       :: 315x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/15/2021 01:54:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,165
            CDSs (total)                      :: 4,076
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 89
            rRNAs                             :: 10, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 10 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 19 of 69
            Pseudo Genes (incomplete)         :: 44 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 10 of 69
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9111
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13376389-rid12479423.denovo.164"
                     /strain="HB27"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="China: Hebei"
                     /collection_date="2017"
     gene            complement(82..1854)
                     /locus_tag="KRL67_003043"
     CDS             complement(82..1854)
                     /locus_tag="KRL67_003043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide reductase activation-like protein"
                     /protein_id="HBH1523909.1"
                     /translation="MKWIYDDYEFENRANNLSWTIAGDYNESIDVSEKDYSSKEIGLY
                     FAIIAGARRKYVDWDIVKKYLINRIRKGYNKDIILGLIQVILNTIVEEKAIKDRPGIE
                     DIRRNAYKDIVSRFTKIHNDNIMDKIKYTFVLYSMDKHPTLDSMTRRIVNDIRNIDTN
                     QDIMEILKALDVIYLTYFEYILNGEENSLIDENNVKSVEVNFDTFADFMYEELYSEEE
                     ENIIENDIDSISSSMLVEGVGDFDDSKSSNSADRVIYVDEDTANKIYSKIEHYYGKTY
                     LSKGEIKKIETQNCRSVHEGCRIHFTDGVLRSECNNVFQLKYVTRQKENNLGKFREHA
                     RMYRQQIKRLKESLARILIEENAKSRIYSDCGTILANRAWRVGRSNNNKIFYKDIENE
                     KGKYVIDILLDASGSQSRNQGNVAIQAYIIANALTEVGIPNRVMGFSSFLDYTILKRF
                     KDYDDSIKNSENIFEYFCAGNNRDGLAIKSICSGLINRDEENKILIVLSDGRPNDVKI
                     GKSSERTLRGEKAYRGIVGLRDTANEVRKARKQNILVLGVFTGKESELEAERLIYGND
                     FIYSRDITRFSDVVSMYLKKIIRN"
     gene            complement(1868..2797)
                     /locus_tag="KRL67_003044"
     CDS             complement(1868..2797)
                     /locus_tag="KRL67_003044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HBH1523910.1"
                     /translation="MDIIDNLRAQGVDEKLIEDVMYFRNYYGLEKDLEYRVTKSKTYF
                     YGKDILSMCISAILEEENILLSGPKATGKNLLADNLGEIFNRPQWNTSFHINTDSSTL
                     IGTDTFIDNEVKLRRGSVYECAVNGGFGVFDEINMAKNDAIVVVHSALDYRRIIDVPG
                     YERVNLHPATRFIGTMNYEYAGTKELNEALVSRFMVIDIPPIEEDKLMMILKNEFSDA
                     DEEKLIQFAGIFLDLQLKSQNGEISSKAIDLRGLMASLKTIRRGLKPTLAINMGLTGK
                     TFDVYEKEMVGDVIKTRIPNKWESIDVFPISHI"
     gene            3187..3375
                     /locus_tag="KRL67_003045"
     CDS             3187..3375
                     /locus_tag="KRL67_003045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HBH1523911.1"
                     /translation="MEYMHCPEIARKHGISERQVQKLCDKGRIPGVSKFEYVWLIPKD
                     VEKPVDGKTKIRKELQYE"
     gene            3368..4069
                     /locus_tag="KRL67_003046"
     CDS             3368..4069
                     /locus_tag="KRL67_003046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBH1523912.1"
                     /translation="MNSVLIIDDDKELCTLMKKCVEQENLSAIIAHSGAEGLHLVDKN
                     KHTLSLVILDIMMPDIDGFQVLKKIRETSNIPVIMLTAKSDEDDKISGLCLGADDYLT
                     KPFSINELMARVNSLIRRYTMLNPAFIADTDCIIVNGMIIDKTNRIVSINNIPIQLTS
                     KEFDLLSFLASNKGRVFTKKQIYTQVWEEEYAFDDSNIMSFISKLRKKIEPDPNRPFY
                     ILTVRGVGYRFNKEA"
     gene            4071..4994
                     /locus_tag="KRL67_003047"
     CDS             4071..4994
                     /locus_tag="KRL67_003047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBH1523913.1"
                     /translation="MNTNVYLLLILVLSLIIIAYLVSALIRVRIQLTLIKDALEDIKN
                     GNLNRRILTDENDITKQICYDINEMAINSQSQLIQQKQSEQAYKQLMTSLSHDVKTPL
                     ASLVGYLEAVESKIVVGDEKDEYIHVASDKAHYLKNFVENLFEWVKLDSKEQVFHFDI
                     FDLNELSRNIIADWIPVLESSHFEYEFDIPEIEYFLHIDANAYTRIINNLLQNIITHS
                     RGDKMTLRIFENKEQAQIVITDNGKGISSDNLPHIFERLYQCDHSRAAKGNGLGLAIA
                     KELINVHKGTITANSIPGMGTEFTILLPKAL"
     gene            5152..6075
                     /locus_tag="KRL67_003048"
     CDS             5152..6075
                     /locus_tag="KRL67_003048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019639192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBH1523914.1"
                     /translation="MNDFVIETKQLTKIYGKQTAVDKVNLHVKKGRIYGLLGRNGAGK
                     TTIMKMILGLASITSGEVDVFGENIKGREKRVYPRIGAIIETPGFYPNLTGTENLEIF
                     AKLRGTAVPNAVKRAMEIVGLPYKNKKLFSKYSLGMKQRLGIANAILHDPELLILDEP
                     TNGLDPIGIAEMRKFIRDLSVEHGKTILISSHILSEISLLADDIGIIDHGVLLEESSM
                     SELEKKNSKYILLQVSDISRASFILECQFNMKDYSVHNDHTLRLYDTTLDMGEINKAL
                     VMQNVTVISSQLCNDTLEDYFKKITGGDGIA"
     gene            6068..6805
                     /locus_tag="KRL67_003049"
     CDS             6068..6805
                     /locus_tag="KRL67_003049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBH1523915.1"
                     /translation="MLKLIQTEFLKLRRRKLIWLMLLSALIMPFFALLYFNYFGKTGV
                     EPIQFYKWSAFSYTLWIILPVVLGILCTMLMYEENKYDMLKQLWIVPISKIGYFFSKF
                     FVVLIYSICFMLTTAVASVLFSVLSGYVVFNWGSILYLLKKCLEIGVITAFVMLPILA
                     IAASQKGYILPVCITLIYVFSGFALMTINMYLHPLSSMGVVIIRNKDIPGITFSQEIN
                     VPLAFLCICIWSIVSLLFANIALGKRK"
     gene            6816..7589
                     /locus_tag="KRL67_003050"
     CDS             6816..7589
                     /locus_tag="KRL67_003050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBH1523916.1"
                     /translation="MWKLLWAECQKIRRSKIVWITVFASIMIAVIVFAGGQDVYNDPD
                     LHYGLKSVYNGSRYIDNAGWYMDEVQPWATFFVLPAVIALLGSYMICREEDEDTIKSL
                     LLIPINEIKLTIAKMIITFMFSILLYLLLFTITFLTESVLHFSDLSTELVLSCMKEYF
                     LDGIGVFLAISPIIALVSRMKKGYWLALVFTEIYSIAGLFAGMSNVLQTFFPITAIFN
                     LSGYYITTVEKIMGSIISLLICVCISAFILKGLKYNEKN"
     gene            7605..7766
                     /locus_tag="KRL67_003051"
     CDS             7605..7766
                     /locus_tag="KRL67_003051"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH1523917.1"
                     /translation="MKKMKKIKKLIILIVFLFLFGMLHCYTEWKFNTDPPKGIIERIN
                     EWTDKIGLE"
     gene            complement(8132..8740)
                     /locus_tag="KRL67_003052"
     CDS             complement(8132..8740)
                     /locus_tag="KRL67_003052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HBH1523918.1"
                     /translation="MSVINSFILQYGLISVFVLIMIEYACFPLPSEVVLPLCGAIAAR
                     NHFGFLTILILSIIAGILGSIFCYTVGNWGGKSIINKIIEICPKAKKGIFASQDYFNK
                     YSSISVCVCRLIPLCRTYISFIAGIAGQNIITFVVSSIVGITIWNTSLISIGYIFSES
                     WVRIMSHYNDYKFLVLIIPISIIFIGFVIKSFIHKKHHKIRL"
     gene            complement(8918..9034)
                     /gene="rrf"
                     /locus_tag="KRL67_003053"
     rRNA            complement(8918..9034)
                     /gene="rrf"
                     /locus_tag="KRL67_003053"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
BASE COUNT         3020 a         1500 c         1297 g         3294 t
ORIGIN      
        1 ttctgtgaaa atttctacat tttttataaa ttttattcat ttttatattt ttatgtatat
       61 aaattctttt ttgtaagttt attaatttct aattatcttc tttaaataca ttgaaactac
      121 atcagaaaat ctagttatat ctcttgaata tataaaatca tttccatata ttaatctttc
      181 agcttcaagt tctgattctt ttcctgtaaa tactcccagt actaatatat tttgctttct
      241 agcttttctt acttcattgg ctgtatctct caaaccaact atacccctat aagccttttc
      301 tcctctcaac gttctctcac tactttttcc tatctttaca tcatttggtc ttccatcgct
      361 gagaactatc aggattttat tttcttcatc tctatttatt agaccactac atatagattt
      421 tattgccaaa ccatctctat tattaccagc acaaaaatat tcaaatatat tttcactatt
      481 ttttatacta tcatcataat ctttaaatct ttttaatatt gtatagtcta aaaaactaga
      541 aaatcccatt actctatttg gtatgccaac ttctgttaga gcatttgcaa ttatgtaagc
      601 ttgaattgcc acatttcctt gatttctact ttgagagcca cttgcatcca ataatatatc
      661 aataacgtat ttcccttttt cattttctat atctttataa aatattttat tattattact
      721 tctaccaact ctccaagctc tatttgctaa tatagtacca caatcagaat atattctact
      781 tttagcattt tcttctatta aaattctggc cagactttct tttaatctct ttatttgctg
      841 tctatacatt ctagcatgtt ccctaaattt tcctaaatta ttttcttttt gtcttgttac
      901 atattttagt tgaaatacat tattacactc acttctaaga actccatctg taaaatgtat
      961 tctacaacct tcatgaacac ttctacaatt ttgtgtctca atttttttaa tttcaccttt
     1021 actcaaataa gtttttccat aataatgttc tatttttgaa tatatcttat ttgcagtgtc
     1081 ttcatcaaca tatataactc ggtctgctga attgctagat ttggaatcat caaaatcccc
     1141 tacaccttct acaagcatgg atgaagatat cgaatctata tcattttcaa taatattttc
     1201 ttcttcttca ctatagagtt cttcatacat gaaatcagca aatgtatcaa aatttacttc
     1261 tacacttttt acattatttt catcaataag actattttct tctccattta atatgtattc
     1321 aaaataagtt aaatatataa catctaatgc ttttaaaatt tccataatat cttgattggt
     1381 atctatattt cttatatcat taactattct tctagtcata ctatctaatg ttgggtgctt
     1441 gtccatacta taaagtacaa atgtgtattt aattttatcc ataatattat cattgtgtat
     1501 tttggtaaaa cgtgaaacaa tatctttata agcatttctt cttatgtcct ctatccctgg
     1561 tctatcttta atggcctttt cttcaactat tgtatttaat attacttgaa ttagacccag
     1621 tataatatct ttgttgtatc ctttccttat tctatttata agatattttt taactatatc
     1681 ccaatcaaca tactttcttc tagcacctgc aattattgca aaatacagac ctatttcctt
     1741 tgaagaataa tctttttctg atacgtctat actctcattg tagtcgccag ctattgtcca
     1801 cgataagtta ttggctctat tttcaaattc ataatcatca tatatccatt tcataatctc
     1861 acctatatta aatatgactt attggaaaca catctatact ctcccattta ttaggtattc
     1921 tagttttaat tacatctcca accatttctt tctcatatac atcaaaagtc tttcctgtaa
     1981 gccccatatt aattgcaagg gttggtttta atcctcttct tatagtctta agagatgcca
     2041 taagaccacg taaatcaata gctttactag atatctcgcc attttgagac ttcaattgta
     2101 aatctaaaaa tattcctgca aattgtatta atttttcttc atcagcatct gaaaattcat
     2161 tttttaaaat catcattaac ttatcttctt ctataggagg aatatctatt accataaatc
     2221 tagataccag tgcttcatta agttcttttg ttcctgcata ttcataattc atagtcccaa
     2281 taaatcttgt tgctggatgt agattgactc tctcataccc aggtacatca attattcttc
     2341 tatagtctaa agctgaatgt accacaacta tagcatcatt ttttgccata ttaatttcat
     2401 caaatacccc aaaacctcca ttaacagcac actcataaac tgaacctctt ctaagtttaa
     2461 cttcattgtc tataaacgta tctgtaccta taagtgttga actgtctgta tttatatgaa
     2521 atgatgtatt ccactgaggt ctattaaata tctcacccaa attatcagca agtaagtttt
     2581 ttcctgttgc ctttggtcct gataataata tattttcttc ttctaaaata gctgatatac
     2641 acatagataa tatatctttt ccataaaaat aagttttaga ctttgttact ctatattcta
     2701 aatctttttc taatccataa taatttctaa aatacattac atcttcaata agcttttcat
     2761 ctaccccttg tgcccttaga ttatctatta tatccattat tcttacccct tttatataaa
     2821 tagtatttgt ttcatttctt atcaccattt tagcataaaa ggatataagc tagaattgat
     2881 tttttaaact aacccacctt aaataagaca atttatcatt ttagcactac aaaaattaat
     2941 aaggacgaca tgattaagaa ggattcaata acaaatctac ttttacataa gaatattaca
     3001 ctactgtaca atgcaattag caagtacaag ataaattttg tagatgtgac aatcacatta
     3061 tatatgttta tacgttctta cgattaacca gaataaagca tattacttac ttatatttct
     3121 tgattttatt cgtatacaca tatataatat atgctgataa attataaaga aaagtcgatt
     3181 ggaaatatgg aatatatgca ttgccctgaa atagcaagaa aacatggaat ttctgaaaga
     3241 caggtacaaa aactatgtga caaaggacgc ataccaggtg tttcaaaatt tgagtatgtg
     3301 tggctaatac caaaagacgt ggaaaaacct gttgatggta aaacaaaaat aagaaaggaa
     3361 ctacaatatg aatagcgttt tgattataga tgatgacaaa gagctttgta ctcttatgaa
     3421 aaaatgtgtg gaacaagaaa atttatctgc tattattgca catagtggtg cagagggact
     3481 acatttagta gataaaaata aacacacact ctctcttgta attttggaca taatgatgcc
     3541 agatatagat ggttttcaag ttctaaaaaa aattagggaa acaagtaata ttccagtgat
     3601 tatgcttacc gctaaaagtg atgaagatga taaaatttcc ggactatgtt taggtgctga
     3661 tgattatcta acaaaaccat tcagcataaa cgaacttatg gctcgtgtca attctcttat
     3721 tcgtaggtat actatgttaa accctgcttt tatagcagat acagattgca taatagtaaa
     3781 tggtatgatt attgataaga caaaccgaat tgtttctatc aataatattc ccattcaact
     3841 tacaagcaaa gagtttgatt tactttcttt tctggcttct aataagggac gtgtatttac
     3901 caaaaaacag atttatacac aagtatggga agaagaatat gcttttgacg atagtaacat
     3961 catgtctttc atcagtaaat tgagaaaaaa gattgaacca gaccctaacc gtccatttta
     4021 tattttgaca gtccgtggtg ttggttatcg ttttaataag gaggcttaac gtgaatacaa
     4081 atgtctatct acttttgata ttagttctta gccttattat tatagcatac cttgtttctg
     4141 ccctgatacg tgtccgaata caacttacac ttataaaaga cgctttagag gatataaaaa
     4201 atggaaacct taatcgccgt attttaacag atgaaaacga cattacaaaa caaatttgct
     4261 atgatattaa cgaaatggca atcaatagtc aatctcaact tattcagcaa aaacaatcag
     4321 aacaagccta caaacagcta atgacaagtc tctcccatga tgtaaaaact ccacttgctt
     4381 ctttggtcgg ttatttggaa gcagtagaaa gtaagattgt tgtaggagat gaaaaagatg
     4441 agtatatcca tgttgcgtct gataaagctc attacttaaa gaattttgta gaaaatctat
     4501 ttgaatgggt aaaattggat tctaaggaac aagtttttca ttttgatatt tttgacttga
     4561 atgaattatc ccgtaatatt atcgctgatt ggatacctgt tttagaaagc agtcattttg
     4621 aatatgaatt tgatatacca gaaatagagt acttcttaca catagacgca aatgcatata
     4681 ctcgcatcat taataaccta ttacaaaata ttattactca tagtaggggt gacaaaatga
     4741 cattgagaat ttttgaaaac aaagagcaag ctcaaattgt aataacagac aatgggaaag
     4801 gcatatcttc tgacaatctc ccacatattt ttgaaaggtt gtaccaatgt gaccattcac
     4861 gggctgcaaa ggggaatggt ttaggcttgg cgattgcgaa ggaacttatt aacgttcata
     4921 agggaactat taccgccaac agcatccctg gtatgggaac agaatttaca atattactcc
     4981 ctaaagccct gtaaaagtac ggggctttct tactctaaaa caagataaaa gcaagattat
     5041 ggcaaggttt tggcaaggtt tatactctat aattctaatt gtagttgatt ttgacgttgt
     5101 atcaactatg attttgtgga agcaaattta ttgaaagtga ggaacttata tatgaatgat
     5161 tttgtgattg aaacaaaaca acttacaaaa atttatggaa agcaaacagc agttgacaaa
     5221 gtcaatctcc atgtaaaaaa aggtcgaatc tatggtctgt tgggacgcaa tggagctgga
     5281 aaaaccacaa ttatgaaaat gattttaggg ttggcttcaa tcacgtctgg cgaggtagat
     5341 gtatttggag agaatattaa aggtcgtgaa aaacgagtat atccacgtat cggtgcaatt
     5401 atcgaaaccc caggctttta tcccaatctg actggtacag agaatttgga gatttttgca
     5461 aaactacgtg gtactgccgt ccccaatgct gtaaaaagag caatggaaat tgtaggctta
     5521 ccttataaaa ataaaaaact gtttagtaaa tattcgcttg gcatgaaaca gcgacttggt
     5581 attgccaacg ctatcctaca tgacccagag ctgttgattt tagatgagcc aaccaacggg
     5641 cttgacccta ttggtattgc agagatgaga aagtttatta gggacttgag cgttgagcac
     5701 ggaaagacaa tccttatttc cagtcatatt ctttcggaaa tctcattgct ggcagatgat
     5761 attggaatta ttgaccacgg cgttcttttg gaagaaagta gtatgagtga actggaaaag
     5821 aaaaacagta aatatattct tctacaagtt tcagatattt cgagagcatc atttatttta
     5881 gaatgtcaat ttaacatgaa ggattattct gttcacaacg accacacctt acgcctttat
     5941 gacactactc tggatatggg tgaaatcaat aaagcacttg taatgcagaa tgtaactgtt
     6001 attagttctc aactttgtaa tgatacttta gaggactact ttaaaaaaat cacaggagga
     6061 gatggcattg cttaaactta ttcaaacaga atttcttaaa ctgcgtcgta gaaaacttat
     6121 ttggctgatg ttgttgtccg ccctcattat gccatttttc gcactcctat acttcaatta
     6181 ttttggtaaa acaggtgttg aaccaataca gttttataaa tggtctgcat ttagttatac
     6241 actttggatt attttacccg ttgttttggg catactttgc accatgctta tgtatgaaga
     6301 aaacaagtat gatatgctca aacaactttg gattgtacct attagtaaaa taggatactt
     6361 ttttagtaaa tttttcgttg ttttaattta ctctatttgt tttatgctta ctacagccgt
     6421 tgcttctgtg ctctttagtg tactttctgg atatgtcgta tttaattggg gaagcatttt
     6481 atatttgttg aaaaagtgtt tagaaattgg tgtaatcacc gcttttgtaa tgttgccgat
     6541 tttggcaatc gctgcatctc aaaagggata cattctccca gtttgtatca cattgatata
     6601 tgttttttct ggctttgctc taatgacaat aaatatgtat ttacatccat tatccagtat
     6661 gggagttgtt atcattagaa ataaagacat tccaggtatt acattttcac aggaaattaa
     6721 cgtaccttta gcatttcttt gtatttgcat ttggagtatt gtatcattat tgtttgcaaa
     6781 cattgcacta ggaaaaagaa agtgaggtgt agataatgtg gaaattactt tgggctgaat
     6841 gtcaaaaaat tcgtcgttca aagattgttt ggattacagt ttttgcatcc attatgatag
     6901 cagtcatagt ttttgcagga gggcaggatg tatataatga cccagattta cattatggct
     6961 taaagagcgt ttataacggt tcacggtata tagacaatgc aggttggtat atggatgagg
     7021 tacaaccttg ggcaactttt tttgttcttc ctgctgtcat tgccctatta ggaagctata
     7081 tgatttgtcg tgaggaagat gaggacacta ttaaatccct gcttcttatt cccataaatg
     7141 aaataaaatt aaccatagca aaaatgatta ttacttttat gttcagtata cttctttatt
     7201 tgttactctt tactataaca tttttgactg aatccgtttt acatttttct gatttatcga
     7261 cagaactggt tttaagttgt atgaaagagt attttcttga tggcataggt gtattccttg
     7321 caatttctcc cattattgcc ttagtatctc gcatgaaaaa aggatattgg cttgcattag
     7381 tattcacaga aatttattct attgctggac tgtttgcggg tatgtcaaat gttttacaaa
     7441 ctttttttcc tattacagca atatttaatc tttcaggata ctatataacc actgtggaaa
     7501 aaattatggg aagtatcata agtttgttga tttgtgtttg catttctgca tttatactga
     7561 aagggctaaa atacaatgaa aaaaattaaa ataggtatct taaaatgaaa aagatgaaaa
     7621 agataaaaaa gctgattata ctaattgtat ttttgtttct ttttggtatg ctacactgtt
     7681 atactgaatg gaaattcaat actgacccac ctaaagggat aatagaaaga ataaatgagt
     7741 ggactgataa gattggactg gagtagtaac ttaagttaat tgtggtgaat gtaatattag
     7801 caagttattg tttcatattt taatactata actttaaata tttggtttta atctgccgaa
     7861 ctaatgtaaa aaaactttca cagaggcaac atatttttga atatttataa agcggtagtt
     7921 ttgattttca aagctgccgc attttctttt atataagcaa acttactccc aacaaatttt
     7981 aaaacatggt acaaaaaaag caccacagac ataagcgata aaagaaacgc cgagaaccta
     8041 caaaaacgta gtgtttatgc agatttacga cgctttctat tttctcaaca gtcttaatga
     8101 ccttgtacaa aagaaatttt tctaatattt tttataatct tatcttgtga tgttttttat
     8161 gtataaatga cttaataaca aatccaataa aaattatgga tattggtata attagaacta
     8221 ggaatttata atcattataa tgtgacatta ttcttaccca actctcagaa aaaatatacc
     8281 ctatactgat taatgaagta ttccatatgg ttattcccac gatagatgat actacaaaag
     8341 ttataatatt ttgacctgct atacctgcta taaaagatat gtatgttcta cataaaggga
     8401 ttaatctaca tacacaaaca gaaatagaag agtatttatt aaaataatct tgagatgcaa
     8461 atattccttt ctttgccttt ggacatatct ctattatttt atttatgata gatttacctc
     8521 cccaatttcc cactgtataa caaaatatcg aaccaagtat acctgcaatt atacttaaaa
     8581 ttaaaattgt caaaaatcca aaatgatttc ttgcagcaat tgctccacat aaaggtaaaa
     8641 caacttcgct aggtaatgga aaacaagcat actcaatcat aattaataca aatacagaga
     8701 ttaatccata ttgtaaaata aaactattga ttacagacac ttacttatct atctcctttc
     8761 ttatttaaag gttttaaggg tacatattct aaggattctc accctatatt taaagaatca
     8821 atctctttaa actattatat gtatttacac aaagcattta taacatactt taaaaatagt
     8881 accttaaaat aagaaactta ataaataaaa aaagactacg tggctacgtc ctactctccc
     8941 aggcagtttc ccaccaagta ccatcagcgc tagagagctt aacttctgtg ttcggaatgg
     9001 gaacaggtgt atcctctctg ctattgtaac cacataatct ttatgcttaa tatttctatt
     9061 aagtttttaa taagtttata gaatctagta atactagttt ctttagagtt a
//
LOCUS       DAEPXF010000158         8916 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain HB27
            SAMN13376389-rid12479423.denovo.165, whole genome shotgun sequence.
ACCESSION   DAEPXF010000158 DAEPXF010000000
VERSION     DAEPXF010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13376389
            Sequence Read Archive: SRR10518432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8916)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8916)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001069624.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001069624.1
            Genome Coverage       :: 315x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/15/2021 01:54:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,165
            CDSs (total)                      :: 4,076
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 89
            rRNAs                             :: 10, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 10 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 19 of 69
            Pseudo Genes (incomplete)         :: 44 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 10 of 69
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8916
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13376389-rid12479423.denovo.165"
                     /strain="HB27"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="China: Hebei"
                     /collection_date="2017"
     gene            complement(82..690)
                     /locus_tag="KRL67_003054"
     CDS             complement(82..690)
                     /locus_tag="KRL67_003054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HBH1523919.1"
                     /translation="MEKDFFRKNGIDLAKSILGKYLIRKYENKVIVTKIIETEAYMGV
                     NDKGAHVYGNKKTDRTKPLYLDGGHIYVYLIYGMYNCLNLSANIENVPECVLIRGVEP
                     ITSLDEISMNRYNKAYTELSKYQVKNITNGPGKLCKALKIDRSLNSKSIMGEELYISD
                     FYYDDKGKKVFSKDELDIKTSKRINIDYAEEAKDFLWRFYIE"
     gene            complement(799..3537)
                     /locus_tag="KRL67_003055"
     CDS             complement(799..3537)
                     /locus_tag="KRL67_003055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing sensor histidine kinase"
                     /protein_id="HBH1523920.1"
                     /translation="MESKGTLSKNKVYILANGLFLVIALLNINLYTVSINMYRIILLW
                     ILGYSIVYISSFKTDEFINILKVGCLVLVFVSILGVVNLKIQKFQIVLLNLSIINMST
                     FIYLTISKVKKLRISPILIFYSAFVILDYFIRNIFSDINWNMIIVLINVCISMFNLLY
                     LLNKLDSNIERYKLIKDLTYFVFFTTIICFVAISKINIVDSDRVIGVIYLLLCNHTYY
                     VYVYSLNNRVVYPYYELDSINKKLSKKSEQLGKINLAIEKDMIIQRTLKNYIDQRKEL
                     LRQALDTIPNVWIVTDYDFNISYTNNKFKDEFSKDMNNYYKILTFIKEDEKVAEKLKK
                     FDNDISIIDKLVELNDKIYLLSLSNNKDESNYLISLNDITNEIKIDEEIRNINKDYES
                     IILNIPSPILVRSAEGGIKKNKVISINKKYEKSFKCASSDLVDMTLEQYFETFNIDFF
                     DNRKFKRLNLTQEKKAEIVSYNDTANCIVNFVMSDSEGEEHVEEVRVGDYWSDNKLFK
                     LLTFRDITKEINILRTVNEQKIIYEKLLDVIPEAIFLEDLSTSRVLYTNRAFRELFGI
                     SSDVLGVTTQKYRNILVKKYINNLNIGEREKSIHIVNENNHIKEVKMISRTLYFGQKR
                     SRVRIIKDLSVQRESERLKKALIKQRQYDQMKMEFYANISHELKTPLNNIYSSVQLIE
                     NLYKKGKIIDFQDILKEHIKITKQNMFRLLRLIDNIINISQVKSDIYKIKAVNFDIID
                     ITERIVTSISSYAKSKGIDLIFDTDEETVMVGLDPESIERIILNILSNAIKFTLEGGE
                     ILVGIYKKDETVEIIIKDTGVGIDKEKLNDIFNRFKQIENSGISNEFGSGIGLCLTKS
                     LIEIQNGKIYIDSKIGEGTNVKIIFPIKEVVEEVYDNSNYNDNIEKFEIEFFDIYK"
     gene            complement(3685..4476)
                     /locus_tag="KRL67_003056"
     CDS             complement(3685..4476)
                     /locus_tag="KRL67_003056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011987989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HBH1523921.1"
                     /translation="MKLKKLLSVALVSAIAISAVGCSNKEDKKILVGASSNPHAKILE
                     VAKPLLKEKGYDLEVKIFDDYVLPNTALDEGSLDANFFQHIPFLEETVKEKGYKLTYT
                     SKVHIEPMGFYSEKVKSLDELKDGAVIAVPNDATNGARALKLLAKNKLIEVKDGELIT
                     KKDITKNPKNIVIKEMNAEQLPTVLKDVDGAVINSNYALTANLNPTKDAIVIESSDSP
                     YVNIIACRENNKDSDKIKALSEAMNSKEVKKFIEDEYKGSIVPAF"
     gene            complement(4512..5168)
                     /locus_tag="KRL67_003057"
     CDS             complement(4512..5168)
                     /locus_tag="KRL67_003057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBH1523922.1"
                     /translation="MNSLIDFLTTLFPSALLQTLYMVIVPTIVATILGFILAIILVVT
                     KPDGLKPNSTINSALGFIVNIFRSFPFMILIVAMIPITRLIVGTSIGETAAIVPITIG
                     AAPFIARIIESSLNEVDKGLIEAAKSFGATKRQIVFKVMIKEAMPSIVSGITLSIISI
                     LGYTAMAGAVGAGGLGNIALIYGYQRFDTAVMVYTVIALVILVQIIQGVGNLAYKKLK
                     "
     gene            complement(5161..6126)
                     /locus_tag="KRL67_003058"
     CDS             complement(5161..6126)
                     /locus_tag="KRL67_003058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein"
                     /protein_id="HBH1523923.1"
                     /translation="MISIKNVNKYYGKVQVLKDVSIEIESGEIFGIIGHSGAGKSTLL
                     RCINGLEEYQEGNVFVSDKEVKSLNEKQMRDLRKELGMIFQHFSLLERRTVFDNVALP
                     LECFGYSKEEIKKRVLELLEVVGISEKKNDKPRNLSGGQKQRVAIARALALNPQVLLC
                     DEATSALDPNTTKSILSLLEDINKKLGITIIVVTHQMEVIKQICGRVAIMENGEVLEV
                     GDTEEIFLRNTKGLRKLIGEESIILPKGTNIKILFPKDISNEAIITTMARELNIDVSI
                     IFGKLEQFKDDILGSLIINISDKSGEQVKQYLTSKGIRWEEMINE"
     regulatory      complement(6232..6338)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            complement(6627..7571)
                     /locus_tag="KRL67_003059"
     CDS             complement(6627..7571)
                     /locus_tag="KRL67_003059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YafY family transcriptional regulator"
                     /protein_id="HBH1523924.1"
                     /translation="MSRAERLIELMITINAKRSFTAGELAEEFSVSKRTILRDLQVLE
                     SVGFPLYSKVGAAGGYHVLKERILPPITFSESEVKSIFFAYQSLEYYNDLPFEQETIS
                     VLKKFLNCIPNDIQYNIENMRRKFVFWTPDRHCSTPLLKELFNIVMNEFTIKIEYSSK
                     QKNSIRTIVPIGLYAMNGLWYCPAYCIKSESVKEFRADRIVKILSIENLSNKKYKVLS
                     SIHDYLKNMEVGTDYHIKINLTDEGVKRCETEFLLARGLKILSKGGYIDMYIPKSTLN
                     WVAEYFLTFGKNATIIEPIELKHSIKSKVLELYNHHCI"
     gene            7682..8149
                     /locus_tag="KRL67_003060"
     CDS             7682..8149
                     /locus_tag="KRL67_003060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH1523925.1"
                     /translation="MENDTIKADDILNYCLSNLDDVVLMDSWGERAIYYNPNGVLKRG
                     VYVLTIKEKDSNNDKGSLVSRPNVYRVNIGLKKETFIEMFGYIPKRPGVGQIVDMDFD
                     FTKLDTVMPHPIYSWMGWICVLSPTEKTFENFKTLIGESYNFAKQKFKKRKNK"
     gene            complement(8317..8916)
                     /locus_tag="KRL67_003061"
     CDS             complement(8317..8916)
                     /locus_tag="KRL67_003061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH1523926.1"
                     /translation="MKLKRTLFVGLALVLAIGITACARDNEKPNENAGKNPNESVNNN
                     EGYMNYYSTSYNDYIAGLNRYSIYDTPESINNQFKDKEYPGNEKYLSDVKAAYKDSRD
                     KIQSFVTSLKKDGKTEDTELKRMNDDLIAEGERLVKDIDERIEKLDNVSDEDMKKGQN
                     DFMKLVHGTEDVGNDIGSGFRKMIKDMNDRLGITGNNKK"
BASE COUNT         2894 a         1501 c          868 g         3653 t
ORIGIN      
        1 aaactaaaaa ttctataaaa aaataagata catacctata aaataccttc ctatagatac
       61 atatcttacc tatataaaaa cttattctat ataaaaacgc cataaaaaat cttttgcttc
      121 ttctgcataa tctatattta tacgtttact agtttttata tctaactcat ctttactaaa
      181 tacttttttc cctttatcat cataataaaa atcacttata tataattctt cacccattat
      241 actcttacta tttagacttc tatctatttt taaagctttg caaagttttc ctggtccatt
      301 agtaatattt tttacttgat atttactcaa ctcagtgtaa gctttgttgt atctattcat
      361 agatatctca tctaaagaag ttataggctc tacccctctt attaatacac actctggtac
      421 attttctata tttgcagata aatttaagca attatacata ccataaatta aataaacata
      481 tatatgtcca ccatctaaat ataatggctt agttctatct gtctttttat tgccatatac
      541 atgtgctcct ttatcattta cacccatgta tgcttctgtc tctatgattt tggtaacaat
      601 tactttattt tcatatttcc ttattagata tttacctaat attgatttgg ctaaatcaat
      661 tccatttttt ctaaaaaaat ccttttccaa aatttcacct cataaaattt atttgcaaca
      721 catcaaatac aactaaagta ataaataaaa ttaactctta ttaatgtgat atatataaac
      781 aataaattta gacatttatt atttatatat atcaaaaaat tctatctcaa acttttcaat
      841 attatcattg taatttgaat tatcatatac ttcttcaaca acctctttaa ttgggaatat
      901 aatttttaca ttagttcctt ctcctatctt actatctatg taaatcttcc cattttgaat
      961 ttcaatcaat gattttgtaa ggcataaacc tataccactt ccaaattcat ttgatatacc
     1021 actattttca atttgtttaa atcgattaaa tatatcattt aatttttctt tgtctatacc
     1081 cactccagta tcttttataa ttatctctac tgtttcgtct tttttatata tacctacaag
     1141 gatttctcct ccttctaaag taaattttat agcatttgat aatatattta gtattattct
     1201 ttctatcgat tctgggtcaa gccctaccat aacagtttct tcatctgtat caaaaattaa
     1261 atctattcct ttagacttag cataactact aatagatgta actattcttt ctgttatgtc
     1321 aataatatca aaattaactg ctttaatctt atatatgtct gacttaactt gagaaatatt
     1381 tattatatta tctattaatc tcaataatct aaacatattt tgtttagtta tttttatatg
     1441 ttcttttagt atatcctgaa aatcaattat ttttcctttt ttataaaggt tttcaataag
     1501 ttgaactgaa ctatatatat tatttaaagg tgtcttaagt tcatgagata tattagcata
     1561 aaactccatc ttcatttggt catactgtct ttgttttatt aatgcttttt taagtctttc
     1621 agactctctt tgcacactta aatctttaat aattctcact ctacttcttt tctgtccaaa
     1681 gtataatgtt cttgatatca tttttacttc tttaatgtga ttattttcat ttactatatg
     1741 gatactcttt tctctttctc ctatatttaa attgtttatg tattttttta ctaatatatt
     1801 tctatatttt tgtgttgtaa ctcctaatac atcactagaa attccgaaca actctctaaa
     1861 ggctctattt gtatatagta cccttgatgt gcttaaatcc tcaagaaaaa tagcctctgg
     1921 tataacatcc agtaactttt catatattat tttttgttca tttactgttc taagaatatt
     1981 tatctcttta gttatatctc taaatgtaag caatttaaaa agtttattat ctgaccaata
     2041 gtctccaact ctaacttctt caacatgctc ttcaccttct gagtcagaca tcacaaaatt
     2101 aactatacag tttgcggtgt cattgtaact tactatttca gctttctttt cttgtgttag
     2161 atttaatctt ttaaactttc tattgtcaaa gaaatctata ttaaaagttt caaaatattg
     2221 ttctaatgtc atatcgacta aatcacttga agcacattta aaactctttt catatttttt
     2281 atttatgctt attaccttat tcttctttat accaccttca gcacttctta caagtatggg
     2341 agatggaata tttagtataa tgctttcata gtctttattt atatttctta tttcttcatc
     2401 tattttgatt tcatttgtaa tatcatttaa acttattaag taattgcttt catctttatt
     2461 attacttaaa cttaacaaat aaattttatc atttaactct actagtttat caattatact
     2521 tatatcgtta tcaaatttct tcaatttttc agcaactttt tcatcttcct ttataaaagt
     2581 taatatttta taataattat tcatatcctt tgaaaattca tctttaaatt tattatttgt
     2641 gtaacttata ttaaaatcat agtctgttac tatccataca tttggtattg tatccagagc
     2701 ttgtctaagt aattcttttc tctggtctat gtagtttttt aacgttcttt gtataatcat
     2761 atccttttct attgctaaat ttatctttcc taattgctct gactttttac ttaatttttt
     2821 atttattgaa tcaagttcat aatatggata cactactcta ttattaagac tgtatacata
     2881 tacataataa gtatggttac ataatagtaa atatattact cctattactc tatctgaatc
     2941 tactatatta atcttagaaa ttgctacaaa acatataata gttgtaaaaa aaacaaaata
     3001 tgttaaatct tttattaact tatagcgctc aatgttacta tcaagtttat tcaacaaata
     3061 tagtaaatta aacatagata tacaaacatt aattaataca ataatcatat tccagttaat
     3121 atcactaaaa atatttctta taaagtaatc taatatcaca aaagcactat aaaaaattag
     3181 tattgggctt attcttagtt tttttacttt agaaatggtt aagtatataa aagtagacat
     3241 attaattatg cttaagttaa gcaacacaat ttggaatttt tgaattttta aatttactac
     3301 tcctaaaata cttacaaaaa ctaatacaag acaccctact ttcaatatat ttataaattc
     3361 atctgtttta aagctactta tataaactat agaatatcca agtatccaaa gtaaaattat
     3421 tctatacata tttatagaaa ctgtatacaa atttatattt aaaagtgcaa taaccaaaaa
     3481 taacccatta gctagtatat agaccttatt cttacttaaa gttcctttag attccatatt
     3541 ccccaccact tcttcatttt tatatagttt tacctataaa tatgttacca caattcttcc
     3601 aaaaaatttg tcactttcta gttataaaaa aaaatgagta aatattttta attttactca
     3661 ttttgtagat tttgtatatt cagtttaaaa agctggaact atacttcctt tatattcatc
     3721 ttctataaat tttttaactt ctttactatt catagcctct gataaagcct taatcttatc
     3781 actatcttta ttattttctc tacaagctat tatatttaca tatggagaat cacttgattc
     3841 tattactatt gcatccttag taggatttag attagctgtt aaggcgtaat tagaatttat
     3901 tactgctccg tcaacatctt ttaatactgt tggtaattgc tctgcattca tttcttttat
     3961 tactatattt ttaggatttt tagttatatc ttttttagtt ataagctctc catcttttac
     4021 ttctatcaat ttattttttg ccaataattt aagtgctcta gctccattag ttgcatcatt
     4081 tggcacagct ataacagctc catcttttaa ttcatctaat gacttaactt tttctgaata
     4141 gaatcccatt ggctctatat gaactttaga tgtataagta agtttatatc ctttctcctt
     4201 cacagtttcc tctaaaaatg gaatatgttg aaagaagtta gcatccaaag acccttcatc
     4261 taaagccgta tttggaagta cataatcatc aaatattttt acttctaaat catatccttt
     4321 ttcttttaac aaaggttttg caacttccaa tatctttgca tgtggatttg atgaagctcc
     4381 tactagtatt tttttatcct ctttgtttga acaacctact gctgatattg ctattgctga
     4441 caccaatgct actgataata acttctttaa tttcattttt actccccctc tttaaaatgt
     4501 ttataattaa tttattttag tttcttataa gccagatttc ctactccctg gattatctga
     4561 actaatatta caagtgctat tacagtatat accattactg ctgtatcaaa tctttgatat
     4621 ccataaataa gcgctatatt acctagtcct ccagctccta ctgcacctgc catagctgta
     4681 taaccaagta tagatattat tgataatgta attccagata ctattgaagg catagcttct
     4741 tttatcataa ctttaaatac tatttgtctt tttgtagctc caaaagattt tgctgcttct
     4801 atcaaccctt tatcaacttc atttaatgag ctttctataa ttctagcgat aaacggtgct
     4861 gccccaatag ttattggcac tattgcagca gtttccccta tactagttcc aacgattaat
     4921 ctagttattg gaatcattgc aactataagt atcataaatg ggaaacttct aaatatgttt
     4981 actataaatc ctaatgcact atttattgtg ctatttggtt ttaatccatc tggttttgta
     5041 actactaata tgattgctaa tataaatcct aatattgttg ccactatagt tggtactata
     5101 accatataca aagtttgtaa caatgcactt ggaaatagtg ttgttaaaaa gtcaattaaa
     5161 ctattcattt atcatttcct cccatcttat gcctttacta gtcagatatt gttttacttg
     5221 ctctccactt ttatctgaaa tgtttataat aagtgaacct aaaatatcgt ctttaaattg
     5281 ttctaatttc ccaaatatta tagacacatc tatatttaat tctcttgcca tagtcgtaat
     5341 aatagcttca ttagaaatat ctttagggaa tagtatcttt atatttgttc cttttggtaa
     5401 tattatactt tcttcaccta ttaattttct aagcccttta gtatttctta agaatatttc
     5461 ttctgtatct cctacttcta atacttctcc attttccatt attgctactc ttccacaaat
     5521 ctgctttata acttccattt gatgagttac aactattatt gttattccca actttttatt
     5581 tatatcctca agcaatgaaa gaattgactt cgtagtattt gggtctaatg ctgaagttgc
     5641 ttcatcacaa agaagtacct gtggatttag tgctaaagct ctagcaattg caaccctttg
     5701 tttttgtccg ccacttagat ttctgggttt atcatttttc ttttcagata ttccaactac
     5761 ttctaataac tctaaaactc tttttttaat ttcttccttt gagtatccaa aacattctaa
     5821 tggcaatgct acattgtcaa atacagttct tctttcaagc aatgaaaagt gttgaaaaat
     5881 cattcctaac tcttttctta aatctctcat ttgcttttca ttcaatgact ttacttcttt
     5941 atcagaaaca aatacattgc cttcttggta ttcttctaat ccgtttatac atctaagtag
     6001 tgttgatttt cctgcaccac tatgacctat tattccaaaa atttctccag attctatttc
     6061 aatacttaca tctttaagca cttgaacctt tccataatac ttattaacat tttttatgct
     6121 aatcataaaa tttcctccca tatattctgc tcagttttta tgtttatcct ctcttatgta
     6181 ttacaacaaa ataaaagccc gagtataaac tcaggcttaa aaagctattt atactcatct
     6241 atcaactata ttagttgcag gatttagcac caatgtatat aaaatatact ggttgccggg
     6301 tttcaaaggg ccagtccctc caccactctt gataagacgt tatttaattt aattctatta
     6361 tcatttgttg ctatgttata tatgatatat tcattaatag tatttgtcaa taaatttatt
     6421 taaatttttt attttttatt atcgttaatt aactattttt aattagtttt ctagctccta
     6481 acactttata ttttaatgtc tatataacta tataacatca ttataagaaa tatattaaat
     6541 aatatcaata tatctaaatc atctgtaaag taaattaaga aaattaacat tttctccaaa
     6601 ctctatccta tcttaaataa aataatttaa atacaatgat gattatatag ctctaatact
     6661 ttagacttta tcgaatgttt aagttctata ggttctatta ttgtagcatt ttttccaaaa
     6721 gttaaaaaat attcagccac ccaattaagt gtagattttg gtatatacat atctatgtat
     6781 ccacccttag acaaaatttt caatcctctt gccaacaaaa attctgtttc acaacgtttt
     6841 actccctcat ctgtcagatt aattttaata tgataatctg tacctacttc catattctta
     6901 agataatcat gtatagaaga taaaacctta tatttcttat ttgataagtt ttctatactc
     6961 aaaatcttca caattctgtc tgcacgaaat tccttaacag attctgattt tatacagtaa
     7021 gctggacaat accaaagtcc attcatagca tacaaaccaa taggaacaat ggtacgtata
     7081 ctattttttt gctttgatga atattcaatt ttaattgtaa attcattcat tactatatta
     7141 aacaattcct tcaataatgg cgttgagcaa tgcctatctg gtgtccaaaa aacaaattta
     7201 cgtctcatat tctcaatatt atattgaata tcatttggta tacaatttaa aaatttcttt
     7261 aaaacagaaa tagtttcttg ctcaaatggt aaatcattat aatactctag tgactgatat
     7321 gcaaagaata tagattttac ctcactctct gaaaatgtaa ttggtggaag tatacgttct
     7381 ttaagtacat gatatcctcc agccgctcca acttttgaat atagtggaaa tccaacactt
     7441 tccaatacct gtaaatctct tagtatagta cgttttgata cagaaaattc ttctgctaac
     7501 tctcccgctg taaaacttct ttttgcattt attgttatca tcagttcaat taaacgctca
     7561 gctcttgaca tattcatact cctaatttaa atatagtgac aataattgtc accattatat
     7621 gataagatat tcttgcatct aaagcaataa taatattaaa gaaagaaggg attgatattt
     7681 tatggaaaat gatactatta aggctgatga tattctcaat tattgtctat caaacttaga
     7741 tgatgttgta ctaatggata gttgggggga acgagcaatt tactacaatc ctaatggtgt
     7801 tttaaagcga ggggtatatg ttcttaccat taaagaaaaa gacagtaata atgataaagg
     7861 ttcgttagtt agtcgtccaa atgtataccg tgtaaatata ggattaaaaa aagaaacttt
     7921 tattgaaatg tttggatata ttccaaagcg tccaggtgta gggcaaatag ttgatatgga
     7981 ttttgatttt acaaaattgg acacagtcat gccacatcct atctactcat ggatgggatg
     8041 gatatgcgtt ctaagcccta ctgaaaagac atttgagaac tttaaaactt taataggaga
     8101 atcttacaat tttgcaaaac aaaaatttaa aaagagaaaa aacaagtaaa aacataaaaa
     8161 aataacctct aaataaacta taatgaaaat atgaggttac aagataaaaa ttagtatctt
     8221 gataacatac aaaaaagcga agccaaaaaa tatattaatt acaaggcttc gctatatttc
     8281 aaatatttat ttctaacttt aaaactattt actctattat tttttattat ttcctgttat
     8341 tcccaatcta tcattcatat cttttatcat ttttctaaaa ccactaccaa tatcgtttcc
     8401 aacatcttca gttccatgaa ctagtttcat aaaatcattt tgaccttttt tcatatcttc
     8461 atcagataca ttatctaatt tttctattct ctcatctata tcttttacta atctttcccc
     8521 ttcagcgatt aggtcatcat tcattctctt taactctgta tcttctgttt taccgtcttt
     8581 tttaagagat gttacaaatg attgtatttt atctctacta tctttatatg cagcctttac
     8641 atcacttaga tatttttcat ttcctggata ttctttatct ttaaactgat tgtttatact
     8701 ttctggagta tcataaatag aatatctatt tagacctgct atataatcat tataacttgt
     8761 tgaataataa ttcatatatc cttcattatt atttacagat tcattaggat tttttcctgc
     8821 attttcattt ggtttttcat tatctctagc acatgcagtt attcctatag ctaatactaa
     8881 tgctaatcct acaaataaag ttctttttaa tttcat
//
LOCUS       DAEPXF010000159         8741 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain HB27
            SAMN13376389-rid12479423.denovo.166, whole genome shotgun sequence.
ACCESSION   DAEPXF010000159 DAEPXF010000000
VERSION     DAEPXF010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13376389
            Sequence Read Archive: SRR10518432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8741)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8741)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001069624.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001069624.1
            Genome Coverage       :: 315x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/15/2021 01:54:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,165
            CDSs (total)                      :: 4,076
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 89
            rRNAs                             :: 10, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 10 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 19 of 69
            Pseudo Genes (incomplete)         :: 44 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 10 of 69
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8741
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13376389-rid12479423.denovo.166"
                     /strain="HB27"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="China: Hebei"
                     /collection_date="2017"
     gene            278..1294
                     /gene="gap"
                     /locus_tag="KRL67_003062"
     CDS             278..1294
                     /gene="gap"
                     /locus_tag="KRL67_003062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011948756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HBH1523927.1"
                     /translation="MKIKVGMNGFGRIGRAVLRIAQEELGDNIEIVAINARATTESLA
                     HLFTYDSCYGTFRGEVEAKDEDTLVVNSKEIKILRYNDPEELPWKELGVDIVIESTGL
                     FTQREKAEKHIKAGAKKVIITAPGKNEDITIVIGVNEKEYDNEKHNIISNASCTTNCL
                     APFAKVLDEKFGIVKGLMTTVHSYTNDQRILDKSHKDLRRARAAAESIIPTTTGAAKA
                     VSRVLPQLEGKLNGFSLRVPTPTVSLVDLVCELKENVTSEQVNSVLKDAAEGELKGVL
                     GYCDKPLVSIDYKGDSRSSIIDALSTMVIEDNMVKVVSWYDNEWGYSSRTVDLVKYVA
                     EKLK"
     gene            1689..2858
                     /locus_tag="KRL67_003063"
     CDS             1689..2858
                     /locus_tag="KRL67_003063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH1523928.1"
                     /translation="MEKLTDRRQVKAVSMETFIFLILLVVGFGYVGSIMGAGMMFKVI
                     MSTAHALLLETVFLIMAMAVLAGALSALLSEFGVIALINKIFAVFMKPLYGLPGASIA
                     GAITTYLSDNPAIIPFAKDKTFTQYFKQYQVPALCNLGTAFGMGLILTTFMISQGTEY
                     VLPALIGNLGAIIGSVISVRIMLTFTKKFYKYNPEEDKATGTLEKKDEFREIREGNVF
                     QRALDAILEGGKMGVDMGMAIIPGVLVVCTLVMLLTFGPSTDPVTGQEVYTGAAYEGI
                     KLLPVIGDKLGFILEPLFGFTSPEAIAFPITSLGAVGAAMSLVPEFIKSGAITPNDIA
                     VFTAMGMCWSGYLSTHIGMMDALNARQLAGKAILSHTIGGLCAGAAAHFIFTLVG"
     gene            3239..7492
                     /locus_tag="KRL67_003064"
     CDS             3239..7492
                     /locus_tag="KRL67_003064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacyl-CoA dehydratase"
                     /protein_id="HBH1523929.1"
                     /translation="MDELFHIGIDVGSTTVKVVVLNNSNNIVHKEYRRHYSDVKKSVK
                     EVLNGIYKKLGNINTTIIITGSGGIGISKKLGVKFVQEVISSTKSIEYFHPETDVVIE
                     LGGEDAKITYLSGGIDQRMNGICAGGTGAFIDQMASLLKTDASGLNELAKGYNVIYPI
                     ASRCGVFAKTDIQPLINDGANQTDIAMSIFNAVVVQTVSVLSCGRKIEGNVAFLGGPL
                     YFLSELREAFKRVLDLKDANIIFPENAQLYIAIGAGLLSVDEDSIQLKSLIEKLDSIK
                     NIEDGEVNLLEPLFKDKNEYEEFSRRHEKEKINYVDINSIKSNCYLGIDAGSTTTKAA
                     LIDEDGRLVYSYYNSNEGNPLKTTIKVINEIYDILPKNIKILSSTVTGYGEGLIKKAL
                     KIDNGEIETIAHYKAAKFFNKDVDFILDIGGQDMKCLKIKDGVIDNIILNEACSSGCG
                     SFLETFASSLSMTIEEFAHEGIYSQNPVDLGSRCTVFMNSRVKQAQKEGASVGDISAG
                     LSYSVIKNALFKVIKIRDLNEIGNNIVVQGGTFYNDLVLRSFEKLIGKNVIRPNISGI
                     MGAFGSALIAKEKYESGYKTSLLSKEELNNIKLEASVTRCKGCSNHCLLTINKFSDNE
                     IFISGNRCEKGEAIYGDKKIGLEKKKPINLFKYKYNRIFRYKPLEKEEAKNGEIGIPR
                     VLNMYEDYPFWFTFFNAMGFRVVLSDRSSKQLYETGITSIASETVCYPGKLVHGHIEN
                     LIAKGIKNIFYPSVTNENKEDSNADNYYNCPVVISYSEVIKNNVENIRNKNINYINPF
                     ISLNDKEKLKKRLYDELSRHFSDIKITKEEINHAVDKATDEQNLFKMEIQAAGERALK
                     EIKERNMKGIVLCGRPYHIDPEINHGMPELINSLDMAVLTEDSICHLANVQRPLRVVD
                     QWVYHSRLYKAASFVRDKNYLELVQLNSFGCGLDAVTTDQVQEILNEKSKIYTIIKID
                     EGNNLGAAKIRLRSLKAAMFERESKNIDVKNIKVNRIQYAKNNQITNKHTILAPQMSP
                     IHFQFLEKAVNLSGYNIEILKDTDSSVIEEGLRYVNNDACYPAIIVVGQLITALKSGK
                     YDLNNTSVTITQTGGGCRATNYIGFLRKAMYDAGFKDVPVIALSVNGIEDSGIMDNIS
                     LKLINRLFMSVVYGDLLMKVLYRVRPYEKVTGSANALYEKWVDICKTSLVKAKISVFT
                     RNIKDIVKEFDNLEILDIKKPKVGLVGEILVKFHPIANNNLVDILEREGAEAVVPDLT
                     NFFLSCAFNTIYKHTHLEGSRKSRMIGEAFIYITGIYQRVYKKALDKSERFYAPANIK
                     NVAKSTEPVVSLGNQTGEGWLLTGEMVELLNEGVENIICMQPFGCLPNHIIGKGSIKE
                     LKRLYKNANIIPIDYDPSASEVNQLNRIKLMLSKAFKNI"
     gene            7641..8135
                     /locus_tag="KRL67_003065"
     CDS             7641..8135
                     /locus_tag="KRL67_003065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HBH1523930.1"
                     /translation="MSLVYDVYKSKLGNLYILSEDGFIVSVYIGDEKFNNLKNEYIKK
                     GIDIKRSKEDLKEAISQIDDYFNGKRKVFNLNIRIEGTEFQKSVYREMLKIPYGETLC
                     YSDIARNINNPKSVRAIGQASKSNKIPIIIPCHRVVGKKDIGGYMGNHSDLKEILLNL
                     ERQS"
     gene            8251..8628
                     /locus_tag="KRL67_003066"
     CDS             8251..8628
                     /locus_tag="KRL67_003066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monooxygenase"
                     /protein_id="HBH1523931.1"
                     /translation="MCDKVIASKYHEKGFNCAESVIKAYNEEFNTDIPVCLGSGLGSG
                     CGVASLCGAVNASNIIIGYVKGRNHEDESTKAKVYAKDLTTTVRKEYGSELCIDLKKD
                     LVACREIMDFAYDSLKETLKKEL"
BASE COUNT         3552 a          914 c         1584 g         2691 t
ORIGIN      
        1 attattttta aaaataagta tagtttaaaa attcaaaaaa aagatggcaa aatatgtccc
       61 aagggacatt gagcaaaata aatgatttca gcttcttcac actaaaaagt acatactaaa
      121 tgaggggtaa aataaagaaa aatgatatac taattgtctt ttgataataa aaaaacatgg
      181 attttgatta tttaatcaag aaaaagtatt gaacaaatga tttatttagt gtacaataat
      241 catgtaaaaa catagtaatg gagggagaca tttcataatg aaaattaagg taggaatgaa
      301 cggatttgga agaataggca gagctgtatt aagaatagct caagaagaat taggagataa
      361 tatagaaatt gtagccataa atgcaagagc aacaacagaa tctttggcac atttatttac
      421 atatgattca tgctatggaa cttttagagg tgaagtagaa gcaaaggatg aagatactct
      481 agtagtaaac tctaaagaga taaaaatatt aagatataat gacccagaag aattaccttg
      541 gaaagaattg ggggttgata tagtaataga atcaacagga ttatttacac aaagagaaaa
      601 ggcagaaaaa catattaaag caggagcaaa aaaagtaatt ataacagctc cgggaaaaaa
      661 tgaagatata actatagtta tcggagttaa tgagaaagaa tatgataatg aaaagcataa
      721 tataatttca aatgcatctt gtactacaaa ttgtctagct ccttttgcta aagtattgga
      781 cgaaaaattt ggtatagtaa aagggttaat gactacagta cattcatata ctaacgatca
      841 aagaatatta gataaaagcc ataaagattt gagaagagca agagcagcgg ctgaatctat
      901 aatacctacc actacaggag cagcaaaagc tgtgtcaaga gtattaccac aattagaagg
      961 aaaattaaat ggtttttcac ttagagtgcc aacaccaaca gtttcacttg ttgatttagt
     1021 atgcgaactt aaagaaaacg ttacatctga gcaagttaac tctgtactaa aagatgctgc
     1081 tgaaggagaa ctaaaaggtg ttttaggata ttgtgataag ccactcgttt ctatagacta
     1141 taaaggagat tcaagatcat ctattataga cgctctatcc acaatggtta tagaagacaa
     1201 tatggttaag gtagtttctt ggtatgataa tgaatgggga tattcttcaa gaactgttga
     1261 cttagtaaaa tatgtagctg aaaaattaaa ataatatata tttcttaaaa caaactttaa
     1321 aaaaattttt taaaaaaatt atataaaatt aaaaaaaaag attatgaaaa atattgaagg
     1381 aaaaattttt tcataatctt ttttctttca tgcaaaaaga tacaagttac aacttatatt
     1441 tgtatattag gtcgttagag aagtatatat atatttagta aggaatcata ctttttataa
     1501 aatgaaagat aagatgtcga aaacttgcat aaatctgaaa aatttttatt attaaaatac
     1561 tgattttcat tttaaaaatg ataaaaaaat gacaaataaa gttgcaaata gtaatactta
     1621 taagtataat taaaaaagag aaaaggtttt gttaaacttt ataacaaaaa aagaagggat
     1681 gattcaaaat ggagaagtta acagacagga gacaggttaa ggcagtaagc atggaaacct
     1741 tcattttcct aatattacta gtagttggtt ttgggtatgt tggtagtatt atgggagcag
     1801 gaatgatgtt caaagtaata atgagtacag cacatgcttt actattagag acagttttct
     1861 taattatggc tatggcagtt cttgcaggcg cattaagcgc actactttca gagtttggag
     1921 taatagcatt aattaataaa attttcgctg tatttatgaa gcctttatat ggactacctg
     1981 gtgctagtat agcaggagct ataacaactt atttatcaga taatccagct attatacctt
     2041 ttgctaaaga taaaacattc acacagtatt ttaaacaata tcaagttcca gcattatgta
     2101 acttaggaac agcctttggt atgggactta tattaacaac atttatgatt tctcaaggta
     2161 cagaatatgt acttccagca ttaattggta atttaggtgc gattataggt agtgttataa
     2221 gtgttagaat aatgttgaca tttactaaga agttttataa atacaatccg gaagaagata
     2281 aagcaacagg aacattagag aaaaaggatg aatttagaga aatcagagaa ggaaatgtat
     2341 ttcaaagagc tttagatgca attcttgaag gtggtaaaat gggggtagat atgggaatgg
     2401 ctataattcc tggagtccta gtagtatgta cattggtgat gttattaact tttggaccat
     2461 ctacagaccc tgttacaggt caagaagttt atacaggagc tgcatatgaa ggaataaaat
     2521 tacttccagt tattggagat aaacttggat ttatattaga accattattt ggatttacat
     2581 caccagaggc aatagcattc cctataactt cattgggagc tgttggtgca gcaatgtctc
     2641 tagttccaga gtttataaag tcaggagcta taactcctaa tgatatagca gtatttacag
     2701 ctatgggtat gtgttggagt ggatatttaa gtacacatat tggtatgatg gatgcattaa
     2761 atgctagaca gttggcaggt aaagcgattc tttctcatac aataggtggt ttatgtgcag
     2821 gagcagcagc tcactttata tttacactcg ttggataaac ataatcagct aagataaaaa
     2881 ataatataca aaaaaatggt tgttgatatg taataagaat ttacatatca acaaccattt
     2941 ttttctgact aaagataatt tataatacat aaacattaag tggatattag agttaagtta
     3001 tatttatgta aaaagtaaca aaaatctgta gaaatttatg acaaaattta gtacactaac
     3061 tatatgttct tatttaaaat ttaaggttag aattaggaat tacctagtag agtaagcttt
     3121 gattaataaa agaatcataa aataaaaata ttagtcataa agattataaa taacttactt
     3181 aaaataaagc catagaactt acaaaaatta aaaaagcata taaatatgga ggggtacaat
     3241 ggatgaatta tttcatatag gaattgatgt aggttctaca acggtgaagg ttgtggtact
     3301 taataatagt aataatatag ttcataaaga atatagaaga cattattctg atgtaaaaaa
     3361 atcagttaaa gaagtactaa atggtatata taaaaaattg ggaaacataa atactaccat
     3421 tataataact ggttcaggtg gaattggaat atcaaaaaaa ttaggcgtaa aatttgttca
     3481 ggaagttata tctagtacaa aatcaataga gtattttcat cctgaaactg atgttgtcat
     3541 agaacttgga ggtgaagatg caaagataac atatctaagt ggaggaatag accaaagaat
     3601 gaatggaata tgtgcaggtg gcactggagc attcatagac caaatggcgt cacttttaaa
     3661 gacagatgcc agtggattaa atgaacttgc taaagggtat aatgttatat atcccatagc
     3721 ttcaagatgt ggtgtttttg ctaaaacaga tatccaacct cttataaatg atggagctaa
     3781 tcagactgat atagcaatga gtattttcaa tgcagttgtc gtacaaactg tgagtgttct
     3841 ttcttgtgga agaaaaatag aaggaaatgt tgcattttta ggtggtccat tgtacttttt
     3901 atctgaactt agagaagcat ttaaaagagt attggattta aaagatgcaa acataatttt
     3961 cccagaaaat gcacaacttt atattgctat tggggcagga ttattatcag tagatgaaga
     4021 tagtatacaa ctaaaatctc taatagaaaa attggatagt ataaaaaata ttgaagatgg
     4081 agaagtaaat ttattagaac ctttatttaa agataaaaat gagtatgaag aattttcaag
     4141 aagacatgaa aaagaaaaaa taaattatgt ggacataaat tcaataaaaa gtaattgtta
     4201 tttaggaatt gacgcaggct caactacaac taaagcagca cttattgatg aggatggaag
     4261 acttgtatat tcttattata atagcaatga aggaaatcca ttaaaaacaa ccattaaagt
     4321 cataaatgaa atatatgata tattaccaaa aaatattaag atattgagtt caactgtaac
     4381 tggatatgga gaaggtctta ttaaaaaagc tcttaaaatc gataatggag agatagaaac
     4441 tatagcgcat tacaaagctg ctaaattttt caataaagat gtagatttta tacttgatat
     4501 tggtggtcaa gatatgaagt gcctaaaaat aaaagatgga gtaattgata atataatatt
     4561 aaatgaagct tgttcatcag gttgtggctc atttttagag acatttgcaa gttctttatc
     4621 tatgacaata gaagaatttg cacatgaagg tatttattct caaaatcctg tagatttagg
     4681 ttcaagatgt actgtattta tgaattctag agtaaaacaa gctcaaaaag aaggcgcaag
     4741 tgttggagat atatcggcag gactatctta ttcagtaata aaaaatgctt tatttaaagt
     4801 aataaagatt agagatttaa atgagattgg taataatatt gttgtacaag gtggaacctt
     4861 ttataatgat ttagtgctta gaagctttga aaaactaatt ggaaaaaatg taattagacc
     4921 taatatttca ggaattatgg gagcttttgg ttcagcactt atagctaaag aaaaatatga
     4981 aagtggatac aaaacgtcct tgttatctaa ggaagagttg aataatatca agctagaagc
     5041 aagtgtaact agatgtaaag ggtgttcaaa tcattgtctt cttacaataa ataaattttc
     5101 cgataatgag atttttattt caggtaatag atgtgagaaa ggtgaagcta tttatggcga
     5161 taaaaaaata ggattagaaa agaaaaaacc aataaattta tttaagtaca aatacaatag
     5221 aatttttaga tacaaaccat tggaaaaaga agaagcaaaa aatggggaaa taggtatacc
     5281 aagagtatta aatatgtatg aagattatcc attttggttt acttttttca atgctatggg
     5341 ctttagagtt gtactttcag ataggtcatc aaaacagtta tatgaaactg gtataactag
     5401 tattgcatct gaaacagttt gttatccagg aaaattagtt catgggcata tagaaaattt
     5461 aattgcaaaa ggtataaaaa atatatttta cccaagtgta acaaatgaaa ataaagagga
     5521 ttcaaatgca gataattatt ataattgtcc tgtcgtaata tcatattctg aagtaataaa
     5581 aaacaatgta gagaatataa gaaataagaa cataaattat ataaatccat ttataagtct
     5641 aaatgacaag gaaaaattga agaaaagact atatgatgaa ttatcaagac atttttctga
     5701 tataaaaata actaaagaag aaattaatca tgcagttgat aaagctactg atgaacagaa
     5761 tttatttaaa atggagatac aagcagcagg tgaaagagca cttaaagaaa taaaagaaag
     5821 aaatatgaag ggaattgtgt tatgtggaag accatatcat atagacccag aaataaatca
     5881 tggaatgcca gagcttataa attcattaga tatggcagtg cttactgaag attctatatg
     5941 tcatttagcc aatgtacaaa gaccacttag ggttgtagac caatgggtat atcattcaag
     6001 attgtataaa gcagctagtt ttgtaagaga taaaaactat ttggaacttg ttcaacttaa
     6061 ttcatttggc tgtgggttag atgcagtaac tacagaccag gttcaagaaa tacttaatga
     6121 aaaatcgaag atatatacta ttataaagat tgacgaaggc aacaatttag gagcagctaa
     6181 aataaggttg aggtctttaa aagcagctat gtttgagaga gaaagcaaaa atatagatgt
     6241 aaaaaatata aaggtaaaca ggatacagta tgctaaaaat aatcagataa cgaacaaaca
     6301 tactatatta gcaccacaaa tgtctccaat acattttcaa ttccttgaaa aagctgttaa
     6361 cttaagtggt tataatatag aaattctaaa agatactgat agtagtgtaa ttgaagaagg
     6421 gttaagatat gtaaataatg atgcatgtta tccagctata atagtagtag gtcagctaat
     6481 aacagcatta aaatctggca aatatgattt aaataatact tctgttacta taactcagac
     6541 tggtggaggt tgtagagcca caaattatat tggattctta agaaaggcta tgtatgatgc
     6601 tggatttaaa gatgttcctg tcattgcact tagtgtaaat ggaatagaag atagtggtat
     6661 aatggataat atatcactaa aattaatcaa tagactcttt atgtctgttg tttatggtga
     6721 tttgctgatg aaagtactat atagagtaag accatatgaa aaagttactg gaagtgcaaa
     6781 tgctctatat gaaaaatggg ttgatatatg caaaacttct cttgttaaag caaaaatttc
     6841 tgtatttact agaaatataa aagatatagt aaaagaattt gataatttag agatactaga
     6901 tatcaaaaaa ccaaaagtag gtttggttgg agaaatattg gtgaaattcc atccaattgc
     6961 aaataataat ttagtagata tattagagcg agaaggtgct gaagcagtcg taccagacct
     7021 tacaaatttc ttcttaagtt gtgcctttaa tactatatac aaacacactc atttagaagg
     7081 aagtagaaag agtagaatga taggagaagc atttatatac ataactggaa tatatcaaag
     7141 agtatataaa aaagcacttg ataagagtga gagattttat gctccagcca atataaaaaa
     7201 tgttgctaaa agtacagaac cagtagtgtc attaggtaat caaaccggag aaggttggct
     7261 tttaacaggt gagatggttg aattattaaa tgaaggtgta gaaaacatta tttgtatgca
     7321 accttttgga tgtttaccta atcacataat tggtaagggt tctataaaag aattaaagag
     7381 gttgtataaa aatgcaaata taatacctat tgattatgac ccttctgcaa gtgaggtaaa
     7441 tcaacttaac aggataaaac ttatgctttc taaggctttt aaaaatatat aagatttata
     7501 ttttcagatg ttaaaagtta atgaatttaa aaatatactc tatattaagt atctataaaa
     7561 aagagttatc ctatttttta ggataactct ttattagtta aaaaattata tgtatactga
     7621 tattaaaggg gggaagtaat atgagtttag tatatgatgt ttataaatca aaattaggaa
     7681 atttgtacat attaagtgaa gatggtttta tagttagtgt atacatagga gatgaaaaat
     7741 ttaataattt aaaaaatgaa tatattaaaa aaggaattga tataaagaga agtaaggaag
     7801 atttaaagga agcaataagt cagatagatg attactttaa tggaaaaaga aaagtcttta
     7861 atttaaatat tcgtattgaa gggactgaat ttcaaaaatc agtttatagg gaaatgttaa
     7921 aaatacctta tggagaaaca ttgtgttatt cagatatagc aagaaatata aacaatccaa
     7981 aatcagtaag agcaatagga caagcaagta aaagtaataa aattccaata ataatacctt
     8041 gtcatagagt agttggcaaa aaagatattg gtggatatat gggaaatcat tctgatttga
     8101 aagagatact attaaattta gaaagacaga gttaacttag aaagaaatgt ttttattgtt
     8161 aatatttgat ataataatag taaaaagaag tattttttac aaaaacaata aagaatttat
     8221 tttaatttac aaaaatgagg aggaatttta atgtgtgata aagttatagc atcaaaatat
     8281 catgagaagg gatttaattg tgcagaatca gttataaaag catataatga ggaatttaat
     8341 acagatatac cagtttgttt aggaagtgga ctgggttcag ggtgtggggt tgctagttta
     8401 tgtggtgcag taaatgcatc aaatataata attggatatg taaaaggcag aaaccacgaa
     8461 gatgaaagta ctaaagctaa agtatatgct aaagatttaa ctacaacagt aagaaaagag
     8521 tatggttcag agttatgtat agacctaaaa aaagatttag ttgcttgtag agaaataatg
     8581 gatttcgcat atgatagtct aaaggaaact ttaaaaaaag aattataaga aaataagcta
     8641 tcaaatatga attatttgat agcttatttt tatagtcgat attaaataaa tcaaatatat
     8701 tttagaatta ttatttgtaa ataaacagca aataatttta g
//
LOCUS       DAEPXF010000160         8654 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain HB27
            SAMN13376389-rid12479423.denovo.167, whole genome shotgun sequence.
ACCESSION   DAEPXF010000160 DAEPXF010000000
VERSION     DAEPXF010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13376389
            Sequence Read Archive: SRR10518432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8654)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8654)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001069624.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001069624.1
            Genome Coverage       :: 315x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/15/2021 01:54:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,165
            CDSs (total)                      :: 4,076
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 89
            rRNAs                             :: 10, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 10 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 19 of 69
            Pseudo Genes (incomplete)         :: 44 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 10 of 69
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8654
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13376389-rid12479423.denovo.167"
                     /strain="HB27"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="China: Hebei"
                     /collection_date="2017"
     gene            65..832
                     /locus_tag="KRL67_003067"
     CDS             65..832
                     /locus_tag="KRL67_003067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HBH1523932.1"
                     /translation="MGEIINALDRALDIILLLYHEKREMGITEISKAMGVYKSTVHRT
                     LVTLENKGFVIQNAENGKYWLGINLYAIGMVVGEKMSLTEIVKPYTKKLNQEFNEVVN
                     VSILEERAQDSPRSIIIHKEYGSNQLLSVNPSVGSSSECYCSAVGKCLMAFNDSIDFE
                     KYRKTPIHKYTEHTIDNWDDMMLFLAKIKEQGYAIDDEELEHGLTCIGAPILDKNNKA
                     IAAISLSGPTIRMREGDFEYKIKRVIETAKSISELFR"
     gene            1116..1313
                     /locus_tag="KRL67_003068"
     CDS             1116..1313
                     /locus_tag="KRL67_003068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HBH1523933.1"
                     /translation="MEKVKLSVERCKGCYLCIEACKKDAISLSDYTNKKGFVTIKVDE
                     EKCIQCGACYTMCPDLVFEIL"
     gene            1342..2418
                     /gene="vorB"
                     /locus_tag="KRL67_003069"
     CDS             1342..2418
                     /gene="vorB"
                     /locus_tag="KRL67_003069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate dehydrogenase subunit
                     VorB"
                     /protein_id="HBH1523934.1"
                     /translation="MQSLLIKGNEALAEGAIRGGCRFYAGYPITPQSEILEMMAWRQK
                     EVGGVFIQGESEIASVNMVMGASALGARAMTSSSGPGFSLKQEGIAYWVSAGLPAVVA
                     CVQRYGIGDGFIGAGQDNYWQAVKGGGNGDYHLIVLAPNSVQENMDMAYESFELAEKY
                     MHPVLILSDATIGQMVEPCIAPPMKEYDMDKAPWAAKGTPVGEKNKVITDITYFEDQD
                     TWQKGYIEKMRKVQEDEQRWEEIEIEDAEVVFVAYGISSRIAEGAVRDARAKGMKLGL
                     IRPKTLWPYPRKAFKKLSDSLKGLVTVEMNMMAQMKEDVIIACDNRFPVYSVATAQYM
                     AEVNQLIDFAQQIIDGKAKQEEVF"
     gene            2421..3140
                     /locus_tag="KRL67_003070"
     CDS             2421..3140
                     /locus_tag="KRL67_003070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate oxidoreductase"
                     /protein_id="HBH1523935.1"
                     /translation="MAKKAPAIMMCENMFCGGCGHSIVDRIIGEVLTEMDICQDTIIC
                     SDIGCNHMFQFSMNVDVVVPPHGKMGAAMAAMKRVRPDKYIFTIAGDGGAYAIGLGET
                     MSAATRNDNVIFLVMNNNMFAMTGGQMAPTTVEGQYTASTPAGRNYADHGSTFDVVKV
                     MGNLDIAYLARGTITNPKEINTTKRYIKKALEKQKEKKGFCLIEVLVPCPTNWGLKPV
                     DAMKRIENELVPKYPLGEFID"
     gene            3153..3692
                     /locus_tag="KRL67_003071"
     CDS             3153..3692
                     /locus_tag="KRL67_003071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HBH1523936.1"
                     /translation="MLNEIICAGFGGQGVLTAGKIIMYVAYKAEKKITWFPSYGNEMR
                     GGSANCNVVVSDEKIANPCVKNPDILLGMNSVSVDKYESLMKKNGYMFVNESLVSKDR
                     KYREDINVIKVPVTEIAQNLGNEKAANICMLGAMVKSLDLFTKEEFESGMCEYFENQG
                     KGKFNSKNVEAFEAGYDYE"
     gene            3849..4907
                     /gene="buk"
                     /locus_tag="KRL67_003072"
     CDS             3849..4907
                     /gene="buk"
                     /locus_tag="KRL67_003072"
                     /EC_number="2.7.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="butyrate kinase"
                     /protein_id="HBH1523937.1"
                     /translation="MKVLIINPGGTSTKISVFQDENEILKKNITHTREDLKNFSKVFD
                     EYDYRKQLIVDILSSENHSINSFDAVVGRGGLMKAIKGGTYTVSEEMIEDMRNEINGE
                     HASNLGALLAKTIADEIGVQSFVVDPVSVDEFDDVSRITGISDIEKASWLHALNHKAV
                     SRKIAQKLGGKYEDYNFIVAHLGSGISIVAHKQGKMIDGSGGRSDGPFSPERSGGLLA
                     YPLIELCYSGKYTHDEMVAKVSSIGGMYDYLGTKDMIKIENRIKEGDEKAKLIMDAFI
                     YQVAKEISMYGASLSGNVDRVILTGGISHSEMVVKGVAEKVSYLAPIEVVPGEMEMEA
                     LALGALRVLNGEEIAKTY"
     gene            4925..5818
                     /locus_tag="KRL67_003073"
     CDS             4925..5818
                     /locus_tag="KRL67_003073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional enoyl-CoA hydratase/phosphate
                     acetyltransferase"
                     /protein_id="HBH1523938.1"
                     /translation="MLKDFNNLLEEAKKLEPVIVSVAVAEDKEVLKAVKSATDLNIIK
                     PILVGDKDKIENISREIGLKAHEIIGCQNPDESVKKAVEIVKNKEAKVLMKGLVNTSV
                     YMRAILNKETGLRTGRLLSLLAVYELPQYHKLLYCTDSGINVSPNLNQKKDIMTNALL
                     TMKSIGMENPKTAILTANEMVDPKIQSTVDANLLVEMEKSGEIPKCIVEGPIAFDVAF
                     DAHAAEHKGIKSNIAGDVDLLVFPNIEAGNILGKSWLRFNKAKWAGIVLGATNPIILG
                     SRSDTAEIKINSIALACLASQ"
     gene            5848..7056
                     /locus_tag="KRL67_003074"
     CDS             5848..7056
                     /locus_tag="KRL67_003074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HBH1523939.1"
                     /translation="MKYAKRMDMVKASAIRDSQKKIAQKVASGGTVISFAAGLPDPNL
                     FPIEEISEITQKVLNERGKFALAYGPTKGEDELLELLVKRMKEEENIDTKAENIIITT
                     GSQQGIALSAMILLEKGDIVVTENPSYLGAINAFRPYECDFLGVDTDEEGMVTDDLDK
                     LLSENSNIKVIYVIPTFQNPTGKTWSLQRRKDFMEVVNKYDVIVIEDNPYGEIRFTPE
                     PLPTLKSLDTKDKVLYLGSFSKVLCPGFRVAWMCGNVDIIDKAELLKQGIDLQSNQFS
                     QVQVIEFLKKYNLNEHIEKIRAEYKKRCLLMLDAMKKHFPEEAKYTEPDGGMFIWVEL
                     PESINVDDLLDKAIDAGVAYVSGESFFANNGPKNTMRLNYTTMSEEQIVKGVEILAEV
                     IKKELNYACE"
     gene            7080..8339
                     /locus_tag="KRL67_003075"
     CDS             7080..8339
                     /locus_tag="KRL67_003075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBH1523940.1"
                     /translation="MGEKENGIKFGKLMFQLVIIAISWELVYIIPFIQYTLYDPILKA
                     LECTNTQLGFLLTIYGLGNIFGAPIGGWLADRFDYRKIILGSVFLNGIVSFLFAFNMN
                     YPFAVATWVGCAITSLVMNYPSMVKILRVIGKDNQGKVYGFNEAMVGVSGVVMGAIFL
                     YIYTCFATPTLGMRWVMISLGILSIAMCPVLWFVIKDVDIKEEKEEAGEKMSAGDFIT
                     VLKSPNTWLVGISIFCVYSFTVTMSYFTPYITSVLGGSVALSGALAIIRQHGLKLFGA
                     PFGGYCADKLKSPTKVLLPIYVFGIAVIVLFLVLPASTPMTIFIALTFVVGILGYMGK
                     GIYYAVQDEVKVPVKYSATTIGIAAALGFSPDVFQFALIGHWIDTYGNKGYTYTFIFQ
                     IAILVIGILSCLYILKVKKRRASKLEA"
BASE COUNT         3360 a          963 c         1728 g         2603 t
ORIGIN      
        1 taatatataa tatttagtag agtgtgggag tagcacttaa tatttgcagg aggagttttt
       61 gtatatggga gaaataataa atgcattaga tagagcccta gacataattt tgctgttata
      121 tcatgaaaag agagaaatgg gaataactga aataagcaag gctatgggag tatataaaag
      181 tactgtacat agaactttgg ttactcttga aaacaaaggt tttgttatac aaaatgcaga
      241 gaatggtaag tattggctag gaataaattt atatgcaata ggtatggttg tgggagaaaa
      301 aatgtcattg actgaaatag taaaaccata tacgaaaaaa ttaaatcaag aatttaacga
      361 agtagttaat gtatctatat tagaagaaag agctcaagat tctccaagaa gtatcataat
      421 acataaagag tatggttcaa atcaattact ttcagtaaat ccatctgttg gttcaagtag
      481 tgaatgttat tgttcagcag ttggtaaatg tcttatggca tttaatgatt ctatagattt
      541 tgaaaaatat agaaaaacac caatacacaa gtatacagaa catacaattg ataattggga
      601 tgatatgatg ctttttttag caaaaataaa agaacaagga tatgctatag atgatgaaga
      661 actagaacat ggactaactt gtattggagc acccatctta gataaaaata ataaagctat
      721 tgcagccata agtttgtcag gtccaactat aagaatgaga gaaggggact ttgagtataa
      781 gattaaaaga gttatagaaa ctgcaaaaag tatatctgaa ctttttagat gatttttaat
      841 ctcgataata aaaagaggct acatttaaac tgttatttgt aaatatcagt ttaaatgtag
      901 tctcttttta tttaatctaa aattaaatat tcttaaaatt taaaatgtta ttgaaatata
      961 taagcgaatg tgttatcttt taattaagca ataaaaaata gaacaaggtt ctatactata
     1021 gaacaaaatt gatgcatata tagtatttat acaaaatata aatattatat atttaaagtt
     1081 aagtaaaaat attacataaa ggggagattt gagaaatgga aaaagtcaag ttgagtgttg
     1141 aaagatgtaa gggatgctat ctttgtattg aagcttgtaa aaaagatgca ataagtcttt
     1201 ctgactacac aaataaaaaa ggctttgtaa ctattaaagt cgatgaagaa aaatgtattc
     1261 aatgtggtgc ttgttataca atgtgccctg atttagtatt tgagatatta taaattattt
     1321 ttattagagg ggagaaaagg tatgcaaagt ttattaatta aaggaaatga agctttagca
     1381 gaaggtgcta tacgtggtgg ctgcagattt tatgcaggtt atccaataac accacaatca
     1441 gaaatattag aaatgatggc atggagacaa aaagaagtcg gaggagtatt tatccaaggt
     1501 gaatcagaaa tagccagtgt aaatatggtt atgggagcaa gtgcacttgg agcaagagct
     1561 atgacttctt catctggtcc aggattttca ttaaaacaag aaggtattgc atattgggta
     1621 tcagctggtt tgccagcagt tgtagcatgt gtgcaaagat atggtatagg tgatggattt
     1681 ataggagctg gtcaagataa ttattggcaa gcagtaaaag gcggtggaaa tggagattat
     1741 catttaatcg tgttggcacc taatagtgtt caagaaaata tggatatggc atatgaatct
     1801 tttgaattag ctgaaaaata tatgcatcca gtacttatac tttctgatgc tacaataggt
     1861 caaatggtag aaccttgtat agcaccacca atgaaagaat acgatatgga taaagctcca
     1921 tgggctgcaa aaggaacacc tgtaggagaa aaaaataaag taataactga tataacatat
     1981 tttgaagacc aagatacatg gcaaaaaggg tatatagaaa aaatgagaaa agttcaagaa
     2041 gatgaacaaa gatgggaaga gatagaaata gaagatgctg aagtggtatt tgtggcatac
     2101 ggtatttcat caagaatagc agaaggtgca gttagagatg ctagagcaaa aggaatgaaa
     2161 ttaggtctaa taagaccaaa aacattgtgg ccttacccaa gaaaagcatt taagaaatta
     2221 agtgattcat tgaagggact tgtaacagtt gaaatgaata tgatggcaca gatgaaggaa
     2281 gatgtaatta tagcatgtga taatagattc ccagtatatt cagtagcaac agctcaatat
     2341 atggcagaag taaatcaatt aatagatttt gctcaacaga ttatagatgg aaaagctaag
     2401 caagaggagg tattttaatt atggctaaaa aagcaccagc aataatgatg tgtgaaaata
     2461 tgttctgtgg aggctgtgga cactctatag tcgatagaat tattggagaa gtattaacag
     2521 aaatggatat atgtcaagat acaattatat gttcagatat aggttgtaat catatgtttc
     2581 aatttagtat gaatgtagat gttgtcgttc cacctcatgg taaaatggga gcagcaatgg
     2641 cagctatgaa aagagttaga ccagataaat atatatttac aatagctgga gatggtggag
     2701 cttatgcaat agggcttgga gaaactatgt cagctgctac tagaaatgat aatgtaatat
     2761 ttcttgttat gaacaataat atgtttgcaa tgacaggagg gcaaatggct ccaactactg
     2821 tagaaggtca atatacagct tctactccag cgggtagaaa ttacgcagat catggttcaa
     2881 catttgatgt tgtaaaagtt atgggaaatt tagatatagc atatctagca agaggaacaa
     2941 taactaaccc taaagaaata aatacaacaa aaagatacat caaaaaagct ctagaaaaac
     3001 aaaaagaaaa gaaaggtttt tgcttaatag aagtgcttgt accatgtcca acaaactggg
     3061 gattaaagcc agtagatgct atgaagagaa ttgaaaatga acttgttcca aagtatccac
     3121 ttggagaatt tattgattag ggaggaaata atatgttgaa tgaaattata tgtgctggtt
     3181 ttggaggaca gggagttctt acagcaggaa aaattataat gtatgttgca tataaggcag
     3241 aaaagaaaat tacatggttc ccatcttatg gaaatgagat gaggggagga agtgcaaatt
     3301 gcaatgttgt tgtaagtgat gaaaaaattg caaatccttg tgtaaaaaat ccagatatac
     3361 tattgggaat gaacagcgta tcagtagata agtatgaaag tttaatgaaa aaaaatggtt
     3421 atatgtttgt caatgaatca ctagtaagta aggatagaaa atacagagaa gacattaatg
     3481 ttataaaagt acctgttact gaaatagcac aaaatttagg taatgaaaaa gctgctaata
     3541 tttgcatgtt aggtgctatg gtaaagagtt tagatttatt tacaaaagaa gaatttgaaa
     3601 gtggtatgtg tgaatacttt gaaaatcaag gcaaaggtaa attcaattct aaaaatgtag
     3661 aagcatttga agctggatac gactacgaat gaatatttta aatatttaaa ataattattg
     3721 aaatacttat ttttatatgt tagtatttaa ttaataaaat atagaacgtc gttctggaca
     3781 ataggacgaa gtctttgggc tgaaatacaa gaacgacgtt ctatatagta gaactagggg
     3841 gtgttaaatt gaaagtttta ataataaatc ctggtggtac atccacaaag atttctgttt
     3901 tccaagatga aaatgaaatt ttgaaaaaaa atataaccca tacaagagaa gatttaaaaa
     3961 atttttctaa agtatttgat gaatatgatt atagaaaaca actgatagta gatattttaa
     4021 gtagtgagaa tcatagtata aattcttttg acgcagtagt tggtcgtggt ggacttatga
     4081 aggctataaa aggtggtacc tatactgtga gtgaagaaat gattgaggat atgagaaatg
     4141 aaataaatgg ggaacatgca tcaaatttag gtgcattatt agctaaaact atagctgatg
     4201 aaataggggt acaatcattt gtagtagacc cagtttctgt agatgaattt gatgatgtat
     4261 cgaggattac aggaatatca gacatagaaa aagcaagttg gttacatgca ttaaaccata
     4321 aagctgtttc aagaaaaatt gcacaaaaac tagggggaaa atacgaggat tataacttta
     4381 ttgtagctca tttaggttca ggaatatcga ttgtagcaca taaacaaggc aaaatgattg
     4441 atggaagtgg aggtaggtct gatggtccat tttctccaga aaggagtggt ggattacttg
     4501 catatcctct tatagaattg tgttactccg gaaaatatac tcatgatgaa atggtcgcaa
     4561 aagtaagctc gattggggga atgtacgatt atttaggtac aaaggatatg ataaaaattg
     4621 aaaacagaat caaggagggt gatgaaaagg caaaacttat aatggatgca tttatttatc
     4681 aagtagcaaa agaaatttct atgtatggag cttcactttc agggaatgta gatagagtta
     4741 ttttaacagg agggatatct cactcagaaa tggtagtcaa aggagtagca gaaaaagttt
     4801 catatctagc tccaatagag gtagtaccag gagagatgga aatggaagca cttgctcttg
     4861 gagcacttag agtactcaac ggagaagaaa tagcaaaaac ttattaatat ttaggagaaa
     4921 gagtatgtta aaagatttta ataatttatt agaagaggct aaaaagcttg aacctgttat
     4981 tgtaagtgtt gctgttgcag aagacaaaga agtattaaag gctgtgaaat cagcaacaga
     5041 tttaaacatt ataaagccaa ttcttgtagg agacaaggat aaaatagaaa atatatcgag
     5101 agaaattgga ttaaaagctc atgagataat aggctgtcaa aatcctgatg aatcagttaa
     5161 aaaagctgta gagatagtta aaaataagga agctaaagtt cttatgaagg gattagtaaa
     5221 tacgagtgtc tatatgagag ctatattaaa taaagaaacg ggtcttagaa caggaagatt
     5281 attgagtttg cttgcagtat atgaattacc tcaatatcat aaacttcttt attgtactga
     5341 tagtggaata aatgtatctc caaacttaaa tcaaaaaaaa gatataatga caaatgcact
     5401 tcttactatg aaaagtatag gaatggaaaa tcctaaaact gctatactta ctgcaaatga
     5461 aatggtagac cctaagatac aatctacagt ggatgccaac ttacttgtag agatggaaaa
     5521 atcgggagaa ataccaaagt gtatagtgga aggtccaata gcatttgatg tagcatttga
     5581 tgcacatgca gcagaacata aaggtattaa aagtaatatt gctggtgatg ttgacttatt
     5641 agtatttccg aatatagaag cagggaatat attaggaaag tcatggctta gatttaataa
     5701 agctaagtgg gcaggtatag ttttaggggc aacaaatccg ataattttag gttcacgttc
     5761 tgacacagct gaaataaaaa taaattcaat agcattagcg tgcctagcat cacaataaga
     5821 aattattatt aaggaggaaa aagtattatg aaatatgcaa aaagaatgga tatggtaaaa
     5881 gcttcagcta taagagattc tcaaaagaaa atagctcaga aggtagcaag tggtggaact
     5941 gttatatctt ttgctgcagg gcttcctgac ccaaatttat ttccaataga agagataagt
     6001 gaaataactc aaaaagtact taatgaaaga ggaaaatttg ccctagcata tggacctact
     6061 aaaggtgaag atgagttgtt agaactatta gtaaaaagaa tgaaagaaga agaaaatatt
     6121 gatacaaaag cagaaaatat tataataact actgggtcac aacaagggat agcattaagt
     6181 gctatgattt tattagaaaa gggagatata gtagttacag aaaatccaag ttacttaggt
     6241 gcaataaatg catttagacc ttatgaatgt gattttttag gagtagatac agatgaagaa
     6301 ggaatggtaa cagatgattt agataaatta ttatctgaaa attctaacat aaaggtgata
     6361 tatgtaatac caacatttca aaatcctaca ggaaaaacat ggtctttaca aagaagaaaa
     6421 gattttatgg aagtcgtgaa taaatatgat gtcatagtaa tagaggataa tccttatgga
     6481 gaaataagat ttacaccaga acctctacct acacttaaat ctcttgatac taaagataaa
     6541 gtattgtacc ttgggtcatt ttcaaaggta ttatgtccag gatttagagt tgcttggatg
     6601 tgtggtaatg tagatatcat agataaggca gaacttttaa aacaaggtat agatttacag
     6661 tcaaatcaat tttcgcaagt acaagtaata gaatttttaa agaaatacaa cttaaatgaa
     6721 catatagaaa aaataagagc agagtataag aaaagatgtc tgctaatgtt agatgctatg
     6781 aagaaacatt tcccagagga agctaaatat acagaaccag atggaggaat gtttatatgg
     6841 gttgaacttc ctgaaagcat aaatgtagat gatttattag ataaagccat tgatgctgga
     6901 gttgcttatg tttctggaga gtccttcttt gcaaataatg gtccaaaaaa tactatgaga
     6961 cttaactata caacaatgtc agaagaacaa atcgttaaag gtgttgagat acttgcagaa
     7021 gttataaaaa aagaactcaa ttatgcttgt gaataaatat aaaaagtgag ggtttaaata
     7081 tgggagaaaa agaaaacggt ataaagtttg gaaagttaat gtttcaactt gttattatag
     7141 caatatcttg ggaattagtt tatattatac catttatcca atatacatta tatgatccaa
     7201 tcctaaaagc attagaatgt acaaacactc aactaggttt tctacttaca atttatgggt
     7261 taggtaatat atttggagct cctataggag gctggttagc agatagattt gattatagaa
     7321 agataatttt aggttctgta tttcttaatg gaatagtatc atttttgttt gcatttaata
     7381 tgaactatcc atttgcagtt gctacttggg taggttgtgc aatcacatca ttagttatga
     7441 actatccttc tatggtaaag atacttagag ttataggaaa agataatcaa ggtaaggttt
     7501 atggatttaa tgaagctatg gttggagttt ctggagtagt aatgggagca atattcttat
     7561 atatttatac atgctttgca actcctacat taggtatgag atgggtaatg atatctttag
     7621 gaatattatc aatagcaatg tgtccagtat tatggtttgt aattaaagat gtagatatta
     7681 aagaagaaaa agaagaagca ggtgaaaaaa tgtctgctgg tgattttata acagtactaa
     7741 aatcacctaa tacttggcta gtaggaataa gtatattctg tgtatattca ttcacagtta
     7801 caatgtctta ttttacacca tatataacat ctgtacttgg aggttctgta gcactatctg
     7861 gtgcattagc aataataaga caacatggtc taaaattgtt tggagcacca tttggaggat
     7921 attgtgcaga caaattaaaa tctcctacaa aagtactttt accaatatat gtatttggta
     7981 tagcagtaat agttttattt ctagtattac ctgcatcaac tccaatgact atattcatag
     8041 ctttaacatt tgtagtaggt atattaggtt acatgggaaa aggtatatat tatgcagtac
     8101 aagatgaagt aaaagttccg gttaaatatt cagcaactac tataggtata gctgcagctt
     8161 taggattttc accagatgta ttccaatttg cattaatagg acactggata gatacttatg
     8221 gcaataaagg ttacacatat acatttatat tccagatagc aattctagtt attggaatac
     8281 tatcttgttt atatatatta aaggttaaaa agagaagagc aagtaaatta gaagcttaaa
     8341 tattaaattt ggtgattaag tatatataaa tacaatactt attttgatga atgtaattgt
     8401 ataaataaca taaaaaatat caatagatta agttttataa taatgtttag agaataaagt
     8461 ataaaaatca aaatgttatc aagttttcta ggttttaaca taaagttacc cctgaaggtg
     8521 taaaattgag tgtcaattat tatctatttt cttacctaca taaacctatt taatttaaat
     8581 tctaagagaa tttcaaaata gatggtagtt gtactcaatt ttagacctca taataaatac
     8641 gaaaatttaa aatc
//
LOCUS       DAEPXF010000161         8550 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain HB27
            SAMN13376389-rid12479423.denovo.168, whole genome shotgun sequence.
ACCESSION   DAEPXF010000161 DAEPXF010000000
VERSION     DAEPXF010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13376389
            Sequence Read Archive: SRR10518432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8550)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8550)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001069624.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001069624.1
            Genome Coverage       :: 315x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/15/2021 01:54:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,165
            CDSs (total)                      :: 4,076
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 89
            rRNAs                             :: 10, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 10 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 19 of 69
            Pseudo Genes (incomplete)         :: 44 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 10 of 69
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8550
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13376389-rid12479423.denovo.168"
                     /strain="HB27"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="China: Hebei"
                     /collection_date="2017"
     gene            complement(123..863)
                     /locus_tag="KRL67_003076"
     CDS             complement(123..863)
                     /locus_tag="KRL67_003076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HBH1523941.1"
                     /translation="MLYDEDEKEENNERWLLTYSDLITLLMIFFVIMYSMSNVDAEKY
                     KQLSQSLNSAFGGSSGVIEGGNSKIEPVVEPGSNDLDTLQNAKFKKVGEEIQKYLNEN
                     GMANSVSLRVQDRGLVISLKDTILFDTGKAVVKDNSRDKIIQIGKMLNEMNSYMRVEG
                     HTDNMSIKNSEFKSNWDLSVMRATNVVQLLIDNAGIAPDKLSAVGYGEFRPIAENSSE
                     EGRSKNRRVDIVLVDSKYDNVENVSKDK"
     gene            complement(884..1672)
                     /locus_tag="KRL67_003077"
     CDS             complement(884..1672)
                     /locus_tag="KRL67_003077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein"
                     /protein_id="HBH1523942.1"
                     /translation="MTTLIFFIVGIASLVVAFVIDGGHVGALFVFSSALVVFGGTIGA
                     VGASTPFPVFKKSIKIMLLAFKNKKPNNIENIIYFKEIATKARKEGLLSIDKEISEND
                     ELDPFIRKGLELMVDGVDAVTIRNILESQIQITSKRHKEGIAIFDSAGGYAPTLGIIG
                     TVMGLVHILGNLSSNPDALGPQISVAFLATLYGVGSANLIWLPIASKLKAINQQEISE
                     RLLVIEAILSILEGDNSNLMTEKLKIFLNSKELIELEEIGGRAE"
     gene            complement(1760..1850)
                     /locus_tag="KRL67_003078"
     tRNA            complement(1760..1850)
                     /locus_tag="KRL67_003078"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1814..1816),aa:Ser,seq:gct)
     gene            2109..2873
                     /locus_tag="KRL67_003079"
     CDS             2109..2873
                     /locus_tag="KRL67_003079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HBH1523943.1"
                     /translation="MLPQERYEKILSILKSDNIIKINDIVQMFDISIETARRDLAHME
                     SIGLLKRVYGGAIPVIASSVELNYVKRKKINSNDKKQIAIKCSEFISTNDTIFMDTGT
                     TVLEVSKNLKDKKNLTVITNSILVANELMNTNIKVYVLGGYLRETEGSFSGPLTLSAI
                     EQFNVDKAILGAGGITLENGISDYHLEEALVRKKMIERSRKSIVVVEPPKFGINSFAS
                     VAPIEHIDTIITSASIDHNIIIEFSQKGIPLVIADN"
     gene            complement(3153..4268)
                     /locus_tag="KRL67_003080"
     CDS             complement(3153..4268)
                     /locus_tag="KRL67_003080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization protein EutH"
                     /protein_id="HBH1523944.1"
                     /translation="MPFDKIILYIMAVGVLLGALDRIIGNKFGLGEQFEEGFNSMGPL
                     ALGMVGIVCLAPVISDVLGPIIIPIFNLCGADPSMFATILANDMGGYPLAMELAHNKE
                     AGMLAGLVVSSMLGCTLVFSIPVGLGLIEYEDRPFFAKGLLIGLITIPFGGVIGGLIA
                     GFNLSMVIINMIPVIILAVILAVGLKFNSTLMINGSLVFGKFISILITIGLGAAAFQE
                     ITGIVLIKGMTPIREGIQVIGSIAIVLLGTFPILHLLVKVLNKPLTIVGGKLGMDATS
                     AAGLVFTLANSIPVYKMMKDMSPKGKVVNTAWLVCATAALGDHLGFTAGVVPEMITPV
                     VIGKLVGGVLAIALAILMTKDLTLEEEQSIKMKKNEV"
     gene            complement(4309..5835)
                     /gene="glpK"
                     /locus_tag="KRL67_003081"
     CDS             complement(4309..5835)
                     /gene="glpK"
                     /locus_tag="KRL67_003081"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HBH1523945.1"
                     /translation="MKYVLAIDQGTTGTRAVLINEKSEFVFSDYMEHTQIYPQSGYVE
                     HDPIEIWNNTKTVSNNVLQQAFENGINESDIKGIGIDNQGETVMLWDKNTGKPLYNAI
                     VWQCRRTYDYVENLKNIPGLENKIIDKTGLIIDPYFSGTKIKWVIDNVKGVKEKIKNG
                     EVLAGTLDSWLIWKMTGGKSFLTDVSTAARTMLFNIRDMDWDRELLDIIGVPRSILAD
                     IMPTSGDFGTTDKEAFCGVSIKITASMVDQPAALFGNGCFEPGMVKNTYGTGCFLYMN
                     IGEELNIAGNGILTTVAWKIGDKVTYAYDGGVYIAGAAIQWLRDGIGVIKNYSETDDM
                     ANSISSTGGVYFVPAFAGIAAPYWDQYARGTMVGITGSTKKEHIVRATLESVALQVKD
                     VVDSMNLASGKKIKMLRVDGGITKNNFTMQFQSDILGIPIEIAENPETTVLGVGYMAG
                     LSCGVWNSIDEIRSEFTISKKYIPNMTNQKREEILLGWNQAVKRAMNWENDTKKVCAN
                     "
     gene            6128..7225
                     /locus_tag="KRL67_003082"
     CDS             6128..7225
                     /locus_tag="KRL67_003082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--glyoxylate aminotransferase family
                     protein"
                     /protein_id="HBH1523946.1"
                     /translation="MKKTYLFSPGPVMVTDKVRNSLLHYDICHRGNEFMDLFKDTQQK
                     INKLYNATSNYYSLIVSGSGTSVNECVLSSIFDKEDAVLLVSNGVFGERLEEILSTYN
                     VKTYKPNFEWAEYPDLDILEKYIIKNSDIKVIAMVFHETSSGMINPVPELGKLAKKYN
                     KIFFVDAISASAGEYIDVEEFNIDIITGVGGKALGAFPGSAYLCAKESILQNIKENQC
                     KNVYLNLYKHYKVAKSSSQTPNTPNVTLIFALNEALTEFLEDDSKIERYKECSAILRN
                     GMEELGLTFLLPDKYMSNTVTSVFLPKEINVNEFILGLEKESGYVVYPGKGKFLDANI
                     FQVANMGEIYPEDCYKFLDILKSRLEIKKVI"
     gene            complement(7801..8313)
                     /locus_tag="KRL67_003083"
     CDS             complement(7801..8313)
                     /locus_tag="KRL67_003083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH1523947.1"
                     /translation="MKVKKFKSGIVMALTVIAIGTSLTNVSAMELNKSKEFETKYNEM
                     KRDFQEEYGKEDKFNITLDDEFTKEEVNGAKGIIKTTNKVNNEVVVLNYFDEMKSGIE
                     FELKPKAEKNAILRDEFQQGYGKEDKFKVVLDDEFTKEEVNGAKGIIKTTDKETGEVI
                     VYNYFDELEK"
BASE COUNT         2777 a         1518 c         1003 g         3252 t
ORIGIN      
        1 ttatatattt aagataccat atattcctac aaaaaggtaa cttactatgt atctattttt
       61 aatttttgca taaaaaagcc atctccttta gaattaattc tgtattgaga cagctaattt
      121 aactatttat cctttgaaac attttctaca ttatcatatt tactatcaac taaaactata
      181 tcgactcttc tattttttga cctaccttct tcagatgaat tttctgctat tggtctaaac
      241 tcaccatatc ctacagcaga taatttatct ggtgctatac ctgcattgtc aataagaagt
      301 tgaacaacat tggttgctct cataactgat aaatcccaat ttgacttaaa ctctgaattt
      361 tttatagaca tattgtctgt atggccttct actctcatgt aactattcat ttcatttaac
      421 atcttaccta tttgaataat cttatctcta gaattatcct ttacaactgc tttccctgtg
      481 tcaaatagta ttgtatcttt aaggcttatt actaatcctc tatcttgaac tcttaatgac
      541 actgaattag ccataccatt ttcatttaaa tacttctgga tttcttcacc aactttttta
      601 aacttagcat tttgcaaagt atctaaatca ttacttcctg gttcaactac tggttctatt
      661 ttgctattac caccttcaat aactccagaa gaaccaccaa aagcagaatt tagagattga
      721 gataattgtt tatatttctc cgcatcaaca ttactcatag aatacattat tacaaagaaa
      781 atcataagta atgttatgag gtctgaataa gttaaaagcc atcgctcatt gttttcttct
      841 ttttcatctt catcatacaa cataaatatt ccatcctttt atattattca gctctaccac
      901 caatttcttc aagttcaatt agttcttttg aatttaaaaa tatttttaat ttttctgtca
      961 ttaaattaga attatcaccc tcaagtatgg ataatattgc ttctatcacc aaaagtcttt
     1021 cacttatttc ctgttgattt atagccttaa gttttgatgc tattggtaac catattaaat
     1081 tggcagaacc aacaccatac aaagtagcca aaaatgcaac agaaatttga ggtcctaatg
     1141 catctggatt acttgaaaga ttgcctaaaa tatgtactaa tcccataact gtacctatta
     1201 tccccagtgt aggtgcatat ccacctgctg aatcaaaaat tgctattcct tctttatgtc
     1261 gttttgatgt tatctgtatc tgtgattcca atatatttct tatagtaact gcatccaccc
     1321 catctaccat aagttcgagc ccctttctaa taaaagggtc taattcatca ttctcactta
     1381 tttctttgtc aatactaaga agcccctctt tcctagcctt tgttgcaatt tctttgaaat
     1441 agattatatt ttctatgtta ttaggctttt tatttttaaa agctaataac attattttta
     1501 tagatttttt aaacacaggg aatggcgtcg aagctccaac agctcctata gttccaccaa
     1561 acacaacaag tgctgatgaa aatacaaata atgctccaac atgtccccca tcaataacaa
     1621 aagcaactac cagagatgct atccccacta tgaaaaaaat taaagtcgtc atctattccc
     1681 tcctttcttt agttatacat atctaatcat ttatgacatt ataaaaagac tatctttaac
     1741 aaaaatagcc tttaaaaatt ggcggagaag gagggattcg aaccctcgca ccggttaccc
     1801 agcctaatcc cttagcaggg gatcctcttg agccacttga gtacttctcc atatctctat
     1861 actagtaaat tatataataa aatcatattt tagtcaattg ttttataaaa tttgagttat
     1921 tttgaattat taaacatcta tttttacttt tttgcttaaa tgtaagtttt aaatatggta
     1981 aaattatctt gcataacact tataatatta tttgataaat atacatacat taacaaatat
     2041 acctaaactc tgacttaatt gtaagttatg ccaaaactaa aataatacta gtccaaggag
     2101 gatattttat gttaccacag gaaagatacg agaagatttt aagtattctc aaatcagata
     2161 atataattaa aataaatgac atagttcaaa tgtttgatat atcaatagaa actgcacgaa
     2221 gagatttagc ccatatggag agtatcggtc ttttaaaaag agtttatggt ggtgctatac
     2281 cagttatagc aagtagcgta gaacttaact atgtcaaaag aaaaaaaata aactcaaacg
     2341 ataaaaaaca gattgctatt aaatgtagtg agtttataag cacaaatgat actattttta
     2401 tggatactgg aactactgta cttgaagttt caaaaaattt gaaagataaa aagaatctaa
     2461 ctgtaataac caactcaatt ttagttgcaa atgaacttat gaatactaat ataaaagtat
     2521 atgtgcttgg tggctatctt agagaaactg aaggttcttt ttctggtccg cttacactgt
     2581 cagctattga acaatttaat gttgataaag ctattttagg tgctggaggt atcactcttg
     2641 aaaatggaat ctctgactat cacttagaag aagcacttgt tagaaaaaaa atgattgaaa
     2701 ggtctagaaa atctatagta gttgtcgaac ctcctaagtt tggtataaat tcatttgcat
     2761 cagtagctcc tatagaacat atagatacta ttattacctc tgctagtata gaccataata
     2821 taataataga gttttctcaa aaaggaatac ctttagtcat agctgataat tagcttgtat
     2881 gattgatttt tagttccttg atgtaataaa ttaaaattaa ggagccaaat aattatttat
     2941 ttagctccat ttatgtctaa actatacttt atattttaat tatgaactac tttcagttaa
     3001 cttatcattt ttagttattt gactgccatt ttatttaatt tataagtttt ggttattcga
     3061 ttatttttta attgtctgtc tttttaattt atttttagtt atctaaactc ctactttaaa
     3121 ataatttaaa tttgacttaa ttattattta ttctagactt catttttctt cattttaata
     3181 ctttgttctt cttccaaagt taaatctttt gtcataagta tggcaagtgc aattgccaat
     3241 actcctccaa ccaattttcc aattacaaca ggtgtaatca tctctggaac tactcctgct
     3301 gtaaatccta aatggtctcc caaagctgct gtagcacaaa ccagccaagc agtatttact
     3361 actttcccct ttggactcat atctttcatc attttatata ctggaatact attggctaaa
     3421 gtaaaaacta atcctgctgc actagtggca tccataccta atttcccacc cactattgta
     3481 agtggtttat taagaacttt aactaataaa tgtaatattg gaaaagtacc taataacact
     3541 attgcaatac ttccaattac ttgaatacct tctcttatag gtgtcattcc ttttataagt
     3601 actatacctg ttatttcttg aaatgctgct gctcccaaac ctatcgttat caatatagat
     3661 ataaattttc caaaaactaa tgacccattt atcataagtg tagaattaaa ttttaatcct
     3721 actgccaata ttactgccaa tattatcact ggtatcatat ttataattac cattgataag
     3781 ttaaatcctg ctattaatcc tcctataaca cctccaaaag gtattgttat caggcctatc
     3841 agtaaccctt ttgcaaagaa tggtctgtct tcatattcta taagacctag tcctactgga
     3901 attgaaaata ctaatgtaca tccaagcatg gatgacacga ccaatcctgc aagcattcct
     3961 gcctctttat tgtgtgcaag ttccatagcc aatggatacc ctcccatatc attcgctaaa
     4021 atagttgcaa acatagatgg gtcagctcca cagagattga agattggtat tattatagga
     4081 cctagtacat ctgaaattac tggagctaaa caaactatac caaccattcc taaagccaat
     4141 ggacccattg aattaaaccc ttcttcaaac tgctctccaa gtccaaattt atttccaatt
     4201 attctatcta acgctcctaa aagcactccc acagccatta tgtataatat aattttatca
     4261 aagggcattt ttaattcctc ctatacatgt aaaatactct attgtaactt aattagcaca
     4321 cacctttttg gtatcgtttt cccaattcat agctcttttt actgcttgat tccatccaag
     4381 taatatttct tctctttttt gattagtcat atttggaata tattttttgc ttattgtaaa
     4441 ttcacttcta atttcatcaa tactgttcca gacaccacag gaaagacctg ccatatatcc
     4501 tactccaagt actgtcgtct cgggattttc tgcaatttca attggtatac ctaatatgtc
     4561 ggattgaaat tgcattgtaa agttattttt agtaattcca ccatctactc tcaacatttt
     4621 tatttttttc cctgaggcta agttcataga atcaacaaca tccttgactt gaagtgcaac
     4681 agactcaaga gtcgctctta ctatatgctc ttttttagta cttcctgtta tcccaaccat
     4741 tgtacctcta gcatattggt cccaataagg tgctgctata cctgcaaatg ctggtacaaa
     4801 atatacacct ccagtacttg atatggaatt tgccatgtca tcagtttctg aataattttt
     4861 tataactcca attccatctc taagccattg tattgcagca cctgctatat acactccacc
     4921 atcataagca tatgtaactt tatctcctat tttccaagca acagttgtca aaataccatt
     4981 acctgctata tttagttcct cacctatatt catatataga aaacatcctg taccataagt
     5041 atttttaacc attcctggtt caaaacatcc attgccaaat aaagctgctg gttggtctac
     5101 cattgaagca gtaattttta tagaaacccc acaaaatgcc tccttatctg tagttccaaa
     5161 atcaccactc gtaggcatta tatctgctaa aatacttcta ggcactccta taatgtcaag
     5221 caactctcta tcccaatcca tatctcttat attaaaaagc attgttcttg ctgctgtaga
     5281 tacatcagtt aaaaaagatt ttcctcctgt cattttccat attaaccaac tatctaatgt
     5341 tcctgcaagt acttctccat ttttaatttt ttcttttacc ccttttacat tatctattac
     5401 ccatttaatt tttgtacctg agaaatatgg gtctataatt aacccagtct tatctattat
     5461 cttattttct aaacctggta tattctttaa attttccaca taatcatacg ttctcctgca
     5521 ctgccatacg atagcattgt aaagtggttt acctgtattt ttatcccata acattactgt
     5581 ttcaccttga ttatctatac caattccttt tatatcactt tcattgattc cattttcaaa
     5641 agcctgttgt aatacattat tactcacagt ttttgtatta ttccaaattt ctatagggtc
     5701 atgttcgaca tatccagatt gtggatatat ttgagtatgt tccatataat cactaaaaac
     5761 aaactctgat ttttcgttta tcaatactgc tcttgttcct gttgttcctt ggtcaattgc
     5821 taatacatat ttcactttaa atgtccccct ttattttcag aattaataaa ttctttttta
     5881 ttttttgaat atataatcta ttcaagttat ttatatttta tatcatcatg tggatttatg
     5941 caatattcat tacaatattt tttgttattt ttaattatcg tgattaaaaa agtattttta
     6001 ttgaaatatt attgcaatat ttattatttt attctataat ctagttaaaa taacacaaaa
     6061 tattttaata tttacacata gtcagtcatt caaaaattta aatttaactt tttaaggagg
     6121 ttttacaatg aaaaaaacgt atctattcag tcctgggcca gtaatggtta cagataaagt
     6181 cagaaattca cttcttcact atgatatatg ccatagagga aatgagttta tggatttatt
     6241 caaagataca caacaaaaaa tcaataaact atataatgca acttctaatt actactcatt
     6301 aattgtatct ggctctggaa catctgtaaa tgaatgtgta ctatcttcta tttttgacaa
     6361 agaagatgca gttctgctag ttagtaatgg tgtttttgga gaaaggctag aagaaatatt
     6421 atctacttat aatgtaaaaa catataagcc taattttgaa tgggcagaat acccggacct
     6481 agacatttta gaaaaataca taattaaaaa ttctgatata aaagtaattg ctatggtgtt
     6541 ccatgaaact agttcaggaa tgataaatcc agttcctgaa ctaggaaaac ttgctaagaa
     6601 gtataacaaa atattttttg tagatgcaat aagtgcatca gcaggtgaat atattgatgt
     6661 ggaagagttt aatatagata taataacagg tgttggtggt aaagctcttg gagcatttcc
     6721 aggttctgca tacctttgtg ctaaagagag tattttacaa aatattaaag aaaatcaatg
     6781 taagaatgtg tatcttaatc tttataaaca ctataaagtt gctaaatcat cttctcaaac
     6841 accaaataca cctaatgtta ctttaatatt tgcccttaat gaagctttaa ctgagttttt
     6901 ggaagatgac agtaaaatag aaagatacaa agaatgttct gctattttaa ggaatggcat
     6961 ggaagaattg ggtctaacat ttttacttcc agataagtat atgtctaata cagtaacatc
     7021 tgtatttcta cctaaagaaa tcaatgtaaa tgagtttata ttagggctag aaaaggaaag
     7081 tggctatgtt gtctatccag gaaaaggcaa attcctagat gctaatatat tccaagttgc
     7141 aaatatggga gaaatatatc cagaagattg ttataaattc ttagatatct taaaatcaag
     7201 attggagata aaaaaagtta tataacagtt tgtttgtaaa atcctacaat atatcagtat
     7261 aaattaaata gtctgatttt ttaaaatttt cctataatat cctataataa attatatttt
     7321 atacttttgt ataagggtga actataatat gtaaagttca cccctatttc aaatcttttt
     7381 cctttagtat aatctttttc ttttaattta ttttatattt ttgatacaat tattcttaca
     7441 tttaattgtt ttttgttttg aaacaatcgt tttttattta atcattttat attcttgata
     7501 caatcattct tacatttaat tattttttat ttttgacaca atcattctta cattctatta
     7561 ttgtttgttt ttgacgcaac tttttttatt cagtcatttg atattttcaa cacaatcatt
     7621 cttacatttt attattgttt gtttttgacg caatcgtttt ttattcagtc atttactatt
     7681 ttcgacacaa tcattcttac attctattat tgtttgtttt tgatacaatc gttttttatt
     7741 cagtcattta ctattctcaa cacaattacc ttttcattca atcaaagtaa tctttaagca
     7801 ttatttttca agctcatcaa agtaattata tacgataacc tcacctgtct ctttatctgt
     7861 agtctttata atgcccttag ctccatttac ctcttctttt gtaaattcat catctaacac
     7921 aactttgaat ttatcttcct ttccataacc ttgttgaaat tcatctctca aaattgcatt
     7981 tttttctgct tttggcttca actcaaattc aatcccagat ttcatttcat caaaataatt
     8041 taatactaca acctcgttat tcactttatt agttgtcttt ataatccctt tagctccatt
     8101 tacttcttct tttgtaaatt catcatccaa tgttatatta aatttgtctt cttttccata
     8161 ttcctcttga aaatccctct tcatttcatt gtatttagtt tcaaactcct tactcttgtt
     8221 taattccata gctgatacat ttgtcaatga tgttccaatt gctattactg ttaatgccat
     8281 tactatacca cttttaaatt tttttacttt catatcttac ctcctaaatt aaatgttctc
     8341 aatgaaatta tataaagaaa atcctaagtt ttacagtttc attcttgtaa ctaaaattac
     8401 tattatgtaa ccaaactttt tgtttcaact attccttaaa agttgctttt ttgaggaata
     8461 gttggagtta aataatgaga ttttatctat atttatagat ttaaaatcaa atttatccat
     8521 ataaataaat acttattttt gcaaaataaa
//
LOCUS       DAEPXF010000162         8400 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain HB27
            SAMN13376389-rid12479423.denovo.169, whole genome shotgun sequence.
ACCESSION   DAEPXF010000162 DAEPXF010000000
VERSION     DAEPXF010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13376389
            Sequence Read Archive: SRR10518432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8400)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8400)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001069624.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001069624.1
            Genome Coverage       :: 315x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/15/2021 01:54:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,165
            CDSs (total)                      :: 4,076
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 89
            rRNAs                             :: 10, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 10 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 19 of 69
            Pseudo Genes (incomplete)         :: 44 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 10 of 69
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8400
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13376389-rid12479423.denovo.169"
                     /strain="HB27"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="China: Hebei"
                     /collection_date="2017"
     gene            444..2114
                     /locus_tag="KRL67_003084"
     CDS             444..2114
                     /locus_tag="KRL67_003084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF and EAL domain-containing protein"
                     /protein_id="HBH1523948.1"
                     /translation="MREESNSLSLDHIEIFFELLSQSTEDYIFFWDINRNKFKISSAI
                     FDEFNLSKEIESDLVNCWSKIVYPDDVQIWKDDIQELLDGKKGEHNLEYRLINKYEEI
                     VWISCRGKVYVSDDPKTILLVGRIKNIGEKNKFDSITGTWNREQFEHRMNYLIKEKIY
                     KNGAMFIMDVDNFKNINEKYGHSYGDKVLRAIATEVLEYIPKDVRLYRLDGDEFAFFY
                     PMCTKETIEKIYEKIHMYTNTQHEIESNKYYCTITAGVAMYPEDGDNYLDLFKHADIA
                     LDIAKISGKNRIKFFSQELYENKLKVISMQQKLRECVENNFNDFELFFQPQVNAVTKE
                     VIGAEVLLRWHSSTYGEVSPVEFIPILEQSNLIIPVGKWIIKEAVKQCKEWHKINPDF
                     KISVNVSYIQLKEDFFRDFIVECLVEYQLRPEFLILELTENCWIPDINLLNDKFISLK
                     GIGVYIAIDDFGTGYSSLNYLKELSVNIIKIERSFVKNITYNSYEYTFLEYIIKLAHI
                     INLKVCVEGIESYEEYDIVKSLGVDIIQGFLFGRPVSASEFYKFQLSK"
     gene            complement(2232..2966)
                     /gene="pflA"
                     /locus_tag="KRL67_003085"
     CDS             complement(2232..2966)
                     /gene="pflA"
                     /locus_tag="KRL67_003085"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase-activating protein"
                     /protein_id="HBH1523949.1"
                     /translation="MIKGKIHSIETFGTVDGPGIRYILFFQGCPLRCKYCHNRDTWDI
                     KSGKEYTVDEIITDALKYTSFMKFSGGGITASGGESTLQPEFLSELFKKAKENDIHTC
                     LDTSGFVDIETIDPVLDNTDLVLLDLKHMVEEKSIDLTGVGMGKALKLARHLESRNIP
                     VWIRHVLVPGITDDVDNLEKLGQFVATLKNVERFELLPYHSMGIHKWESLGIDYELKD
                     VPDATKEDVQKASEIISKFGVKVYNS"
     gene            complement(3126..5357)
                     /gene="pflB"
                     /locus_tag="KRL67_003086"
     CDS             complement(3126..5357)
                     /gene="pflB"
                     /locus_tag="KRL67_003086"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HBH1523950.1"
                     /translation="MNAWQGFKTGRWTKEINVREFIQLNYSPYEGNDSFLAGATENTK
                     KLWDEAMVLFKKERENGGTLDVDTKTVSGIAAYAPGYLDKELETIVGLQTDAPLKRAV
                     MPYGGIKMVENSCEAFGYELDPEIKDIFTKYRKTHNQGVFDVYTPEMRAARKSGIITG
                     LPDAYGRGRIIGDYRRVALYGVDALIEDKNEQKKSLEVSCMDEEVIRLREEITEQISA
                     LNELKKMAESYGFDISKPATNSKEAVQWLYFGYLGAVKDQNGAAMSLGRTSTFLDIYF
                     ERDLKAGIVTEEELQEYMDHFVMKLRMVKFLRTPDYNNLFSGDPTWVTECIGGMGIDG
                     RTLVTKNSFRMLNTLYTLGPSPEPNLTVLWSTKLPQGFKDFCSKVSIDTSSVQYENDD
                     LMRAYWGDDYGIACCVSAMRIGKQMQFFGARVNLAKTLLYAINGGVDEKSGVQVGPRF
                     EPITSEYLDYDEVMSKFEPFTDWLATLYVNTLNVIHYMHDKYSYEALEMALHDRDIFR
                     TMACGMAGLSVCADSLSAIKHAKVKTIRNEQGIAVDFEIEGDYPKYGNNDDRVDNIAV
                     ELVESFMNKIRKNKTYRNSYPTQSILTITSNVVYGKKTGNTPDGRRAGAPFAPGANPM
                     HGRDTNGALASLSSVAKLPYEHAQDGISNTFSIVPAALGKDMTERINNLSAMMDGYFA
                     QNAHHLNVNVFDRATLEDAMEHPEEYPQLTIRVSGYAVNFIKLTKEQQLDVINRTFHG
                     KMA"
     gene            5649..6710
                     /locus_tag="KRL67_003087"
     CDS             5649..6710
                     /locus_tag="KRL67_003087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium/sodium antiporter"
                     /protein_id="HBH1523951.1"
                     /translation="MEKRKSIHLHKLNIYLIGGGDRLFITVILFLVGFLLITKGADIF
                     INCTVEIGRKTNISEIILGATIVSFATTLPEFTVSLLASIDGHTTMSLGNAVGSIICN
                     TGLALGLVVFISPFNVDKKMFFSKSLLLIVSVIVLILLSLDGVITRGDSLLLIIILIF
                     YMVNNYRSVVGKSDTKNRNIKNNAIKDISTKKGKNYRGFSVLEIVKILLLFATGLIMM
                     IIGSQILIESGVIIASFLNIPQGIVSLTIIALGTSLPEIVSSITAIRKNHHEISVGNI
                     LGANILNIVSVIAVSAIPNNIPILSQNRQLDIPFMILLLLIVIIPTLKSNRLSRIQGI
                     LMLFTYFLYISILYFMYII"
     gene            6801..>8400
                     /locus_tag="KRL67_003088"
     CDS             6801..>8400
                     /locus_tag="KRL67_003088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HBH1523952.1"
                     /translation="MNITKFEDLPISEGIKKAIAEMGFEEPSPIQAQSIPAILSGKDV
                     IGQAQTGTGKTAAFSIPILETIDPNNRSLQAVVLCPTRELAIQVSTEIRKLAKYSHGI
                     KTLPIYGGQPIDRQIKSLKSGVQVVIGTPGRTIDHINRKTLKMDNVKMIILDEADEML
                     DMGFREDIEMILSKIPEERQTTFFSATMPRGILELTKRYQKDPEHIKVVRKELTVSNT
                     KQYYIETRSSNKLEVLCRLVDVYDPKLSVVFCNTKRKADELVGDLQARGYFADALHGD
                     LKQTQRDIVMDKFRNGTIDILVATDVAARGIDVDDVECVFNYDLPQDEEYYVHRIGRT
                     GRAGREGMSFTFVFGKEMRKMKDIERYTKSKLIKHNIPTITDVEEKKVGTFFAQVKQT
                     IEEGHLTKQLQWLEGFCNDEDYAMVDIAAALVKLSLGEEMKEEIIEEKPRRERGDRKG
                     GTGAKDGMIRLFINIGRNQRVQAKDIVGAIAGEVGIPGKVVGTIDIYDKYTFVEIPKK
                     DAKTVIEKMKDIKIKGNKINIEKAN"
BASE COUNT         3104 a         1121 c         1317 g         2858 t
ORIGIN      
        1 ttatagataa tgaagaaata tcaaaatctt atattatagg aagaggcact tctttaccaa
       61 aatctataga aaaacaatac caaatccaaa gacaataggt ggagaagata aaaataaaat
      121 aaattcaaga gtaatagaag agagttttat atgataaaga actgagaact ctttatataa
      181 cgaaaaatag aattttaata gaaaatgatt taatagatga tttaacatgg tgttttagca
      241 ggtaaaaata aatcaccagt aatgattata gaaaaattac tttacgaatc tcaaaaaact
      301 tagttaagag taagggattt gataagataa ctaaagttgg gaaatgtgtt tgatgagata
      361 aaaaaattag taaaatagta taatctaaat aagaatattt tgttgattaa actacactaa
      421 tagtaatatt gtagggggaa aagatgagag aagaatcgaa ctctttaagt ttagaccata
      481 tagaaatttt ctttgaactt ttatctcaaa gtacagaaga ttatatattt ttttgggata
      541 taaataggaa taaatttaaa atttcttcag caatttttga tgaatttaac ttaagtaagg
      601 agatagaatc tgatttagtc aattgttgga gtaaaattgt ttatccagat gatgttcaaa
      661 tatggaaaga tgatatacaa gaattactag atggaaaaaa aggtgagcac aatttagaat
      721 ataggttgat taataaatat gaagaaatag tatggatttc ttgtagaggt aaagtttatg
      781 tgtcagatga cccaaaaact atacttctag tcgggagaat aaagaatatt ggtgaaaaaa
      841 acaaatttga ttctatcaca ggaacgtgga atagagagca atttgaacat agaatgaatt
      901 atcttataaa agagaaaatt tataaaaatg gtgctatgtt tatcatggat gttgataatt
      961 ttaaaaatat aaatgaaaaa tatggacatt cttatggtga caaagtattg cgtgcaattg
     1021 ctactgaagt attggaatat atcccaaaag atgtaagatt gtataggtta gatggagatg
     1081 aatttgcatt tttttaccct atgtgtacta aagaaactat agagaaaata tatgaaaaga
     1141 tacatatgta tacaaatact cagcatgaaa ttgaatcaaa taagtactat tgcacaataa
     1201 ctgcaggagt tgctatgtat ccagaagatg gagataatta tttagattta tttaaacatg
     1261 ctgatattgc attggatata gcaaaaatta gtggtaaaaa tagaattaag tttttttcac
     1321 aagagctcta tgaaaataag ctaaaggtaa tttcaatgca acaaaaacta agagagtgcg
     1381 ttgaaaataa ttttaatgac tttgagctgt tttttcaacc ccaggtaaat gctgttacaa
     1441 aagaggtaat aggggcagag gtacttttaa gatggcattc gagtacttat ggagaagtat
     1501 ctcctgttga atttatacca atactagaac agagtaattt aattatacca gttgggaagt
     1561 ggattataaa agaagctgta aaacagtgta aagaatggca taaaataaat ccagacttta
     1621 aaatatctgt aaatgtatcg tatattcagt taaaggaaga tttttttaga gattttatag
     1681 tagaatgttt agtagagtat caattaagac ctgaattttt aattttggaa ttaacagaga
     1741 attgttggat accagatata aatttattaa atgataaatt tattagttta aagggtatag
     1801 gagtatatat tgcaatagat gactttggta caggatattc atctttaaat taccttaaag
     1861 aactgtctgt taatataata aaaattgaaa gaagttttgt taagaatatt acatacaata
     1921 gttacgaata tacttttctt gaatatatta ttaaattagc tcatattata aatttaaaag
     1981 tttgtgttga gggaatagag tcttatgaag agtatgatat agtaaaaagc ttaggtgtag
     2041 atattattca gggtttttta tttggaagac ctgtaagtgc ctctgaattt tataaatttc
     2101 aactatcaaa gtgatgtata taaaatgaga tatatagtat ataaaaatag ggtaaagtgg
     2161 aagttttgaa gtctattaaa aagttacact ttaccctatt ttgttatgtc ttctataaag
     2221 attaatatat actaactatt atatactttc actccaaatt tagagattat ttcactagct
     2281 ttttggacat cttccttagt ggcatcagga acatctttta gttcataatc gatacctaaa
     2341 ctttcccatt tgtgtattcc catagaatgg tatggtagca attcaaatct ttctacattt
     2401 ttcaatgtag ctacaaattg acctagtttt tctaaattat ctacatcatc agtaatgcca
     2461 ggaactaaaa catgtcttat ccatacagga atatttcttg attctaagtg tctagcaagt
     2521 tttaatgcct tacccatacc aacaccagtc aaatcaatag atttttcctc aaccatatgt
     2581 tttaaatcaa gtaaaaccaa atcagtatta tctaaaactg ggtctatagt ttctatatct
     2641 acgaatccag atgtatccaa gcaagtgtgt atatcatttt cttttgcttt tttaaataat
     2701 tcacttaaaa attctggttg aagagtggat tcaccaccag aggcagttat acctccacca
     2761 gaaaacttca taaaagaagt atacttaaga gcatccgtta ttatttcatc aaccgtgtat
     2821 tcttttcctg atttaatatc ccatgtatcc ctgttatgac agtatttgca tctaagagga
     2881 cacccctgaa aaaataatat atatcttatt cctggtccat ctacagtacc aaatgtttct
     2941 atagaatgta tttttccctt aatcatagag ttcacctcac aatatatttt aataatctta
     3001 tcaatagtta taatatttct ctaaaaaagg ccatcttata ccagaaataa ttcaaatata
     3061 agatagcctt ttatttttat tttttacaca gtagtaatct tagttaaagt ataaaaattt
     3121 aaagactaag ccattttacc gtggaatgtt ctgtttataa catctaattg ttgctcttta
     3181 gttaatttta tgaagttaac tgcatatcca gatactctta tagttaattg tggatattct
     3241 tctggatgtt ccatagcatc ttctaaagta gctctatcaa atacatttac atttaagtga
     3301 tgagcatttt gagcgaagta tccatccatc attgcagaaa gattgtttat tctttcagtc
     3361 atatcttttc ctaaagcagc aggtactata gagaaagtat tagatatacc atcttgagca
     3421 tgctcatatg gtaatttagc aactgaagat aatgaagcta aagcaccatt agtatctcta
     3481 ccatgcattg ggttagcacc aggagcaaat ggagcaccag ctcttctacc atctggtgta
     3541 ttaccagttt tcttaccata aacaacattt gaagttattg taagtataga ttgagttgga
     3601 taagaatttc tataagtttt attctttctt attttattca taaagctttc aactaactct
     3661 acagctatat tatcaactct atcatcattg tttccgtatt ttggataatc tccttctatt
     3721 tcaaaatcaa cagctattcc ttgttcattt ctaatagttt taaccttagc atgttttata
     3781 gcagataatg agtctgcaca aactgataac ccagccatac cacaagccat agttctaaat
     3841 atatctctat catgtaatgc catttctaat gcttcataag aatatttatc atgcatataa
     3901 tgtattacat ttaaagtatt tacatataat gttgctaacc aatcagtgaa tggttcaaat
     3961 ttactcataa cttcatcata atctaggtac tcagaagtta taggctcaaa tcttggtcca
     4021 acttgaacac cagatttctc atctacacca ccatttatag cgtataataa agttttagct
     4081 aagttaactc tagctccgaa gaactgcatt tgtttaccaa ttctcattgc agatacacaa
     4141 caagctatac catagtcatc tccccagtat gctctcatta aatcatcatt ttcatactgt
     4201 actgaacttg tatctataga tactttagaa cagaaatctt tgaatccttg aggtaattta
     4261 gttgaccata gaactgttaa gtttggttct ggagaaggtc ctaatgtata tagagtattt
     4321 aacattctaa atgagttttt agtaactaat gttctaccat ctattcccat acctcctata
     4381 cactcagtta cccaagttgg gtctccagag aataggttat tgtaatcagg agttcttagg
     4441 aacttaacca ttcttaattt cataacaaaa tggtccatat actcttgtaa ttcttcttct
     4501 gttacgattc cagcttttaa atctctttca aagtatatat ctaagaaagt agatgttcta
     4561 cctaaagaca ttgcagcacc attttggtct ttaacagcac ctaaataacc aaagtataac
     4621 cattgtacag cttcttttga gttagttgct ggttttgata tatcaaatcc ataagattca
     4681 gccatttttt taagttcatt taaagctgat atttgctcag ttatttcttc tcttaatctg
     4741 ataacttcct catccatgca agaaacttct aatgatttct tttgctcatt tttatcttct
     4801 attaaagcat caacaccgta tagagcaact cttctgtagt caccaattat tctacctcta
     4861 ccataagcat ctggaagacc tgttattata ccagattttc tagcagctct catttctgga
     4921 gtataaacat caaaaactcc ttggttgtgt gtttttctgt atttagtaaa tatatctttt
     4981 atttctgggt ctaattcata tccaaaagct tcacatgaat tttcaaccat cttgatacca
     5041 ccatatggca tgacagctct ttttaaaggg gcatccgttt gtaacccaac tattgtttct
     5101 aattctttat ctaaatatcc tggagcatat gcagctattc cagaaacagt ttttgtatca
     5161 acatctaaag taccaccatt ttctctttct tttttaaata ggaccattgc ttcgtcccat
     5221 aattttttag tattttcagt agcaccagct aagaaagagt catttccctc atatggagaa
     5281 tagtttaatt gtataaactc tctaacattt atttctttag tccatcttcc tgttttaaat
     5341 ccttgccatg cattcattaa gtatttcctc cttagattta aatgataaat aatgctatta
     5401 aaattaattt ttatatactt aatttatttt atatattttt ttaaaaatat attttctaat
     5461 ataataatag catattattc gtatcctgta tacataaaaa taaaaaattg tcgctttaaa
     5521 gtataaaaca gattatatgt taataatgtg gtgaaattat acccatatat aaataaaata
     5581 aaacaaataa atagctaatt aaggagaaaa tattttttat tttttgttta ggataaataa
     5641 attttattat ggaaaaaaga aaaagcatac atcttcacaa actgaatatt tatttaatag
     5701 ggggaggtga tagattgttt atcacagtga tattattttt agtaggtttc ctattaatta
     5761 ctaaaggcgc agatatcttt ataaattgta ctgtagagat aggtagaaaa acgaatatat
     5821 cagaaattat acttggagct actatagtta gttttgcaac aacattacct gaatttacag
     5881 tgtctctact tgcttctatt gatggtcata ctactatgag tttaggaaat gcagttggtt
     5941 ctataatatg taatacagga ttagcattag ggttggttgt ttttataagt ccatttaatg
     6001 tagataaaaa aatgtttttt tctaagtctt tactattaat agtatccgtt atagttttaa
     6061 ttttattgag tttagatgga gttataacaa gaggtgattc attattactt ataataatat
     6121 taatctttta tatggttaat aattatagaa gtgtcgttgg aaaatctgat actaaaaata
     6181 gaaatattaa aaataacgct attaaagaca tatctactaa aaaagggaaa aattataggg
     6241 gtttttccgt attagaaatt gtaaaaatac tattactttt tgctacagga ttgataatga
     6301 tgataatcgg ttcacaaatc cttatagaga gtggagtaat aatagctagt tttttaaata
     6361 tacctcaagg tatagtaagt ttaactataa tagcacttgg aacttctctg ccagagatag
     6421 tttcgtcgat tactgctata agaaaaaatc atcatgaaat atcagtagga aatattttgg
     6481 gtgcaaacat attgaatata gtatcagtaa tagctgtatc agcaatacca aataacatac
     6541 caatactatc tcaaaataga caattggata taccattcat gatattatta ttattaatag
     6601 taatcatacc aacacttaaa tctaatagac ttagtagaat acaaggtata ttgatgttat
     6661 tcacatattt tctgtacata tcaattttgt atttcatgta tattatttaa ctatactgtt
     6721 taaaatatta attgcagaat atcctaaaaa agtatataat ggataggtta agattaattg
     6781 ataacggaga ggaataatat atgaatataa caaagtttga agatttacca ataagtgagg
     6841 gaataaaaaa agctatagct gaaatggggt ttgaagaacc atctcctata caagcacagt
     6901 ctatacctgc gattttgtca ggtaaagacg ttataggaca ggctcaaact ggtacgggaa
     6961 aaacagctgc ttttagtatc cctatactag aaacaataga cccaaataat agaagtttac
     7021 aagctgtagt gctttgtcca acaagagaac tggcaataca agtatcaaca gaaataagaa
     7081 agttagctaa gtattcgcac ggaataaaga cattacctat atatggtggt cagccaatag
     7141 atagacaaat aaaatctcta aagagtggtg tgcaagttgt aataggtaca ccaggacgta
     7201 caatagacca tatcaatcgt aaaacattaa agatggataa tgtaaaaatg attatattag
     7261 atgaagcaga tgaaatgtta gatatgggat ttagagaaga tatagaaatg attttaagta
     7321 agattccaga agaaagacaa acaactttct tttcagctac tatgccaaga ggaattttgg
     7381 aattaactaa gagatatcaa aaagacccag agcatataaa agttgttaga aaagaactta
     7441 cagtttcaaa cacaaagcaa tactatatag aaactaggtc atcaaataaa ttagaagtat
     7501 tatgtagatt agtagatgta tatgacccta aactatctgt tgttttctgt aatactaaga
     7561 gaaaagcaga cgaattggta ggagatttac aagcaagagg atattttgca gatgcattac
     7621 atggtgactt aaaacagact caaagagata tagtaatgga taagtttaga aatggtacaa
     7681 tagatatact tgttgctact gatgttgcag ctagaggtat agatgttgat gatgttgaat
     7741 gtgtattcaa ctatgattta ccacaagatg aagagtatta cgttcataga attggtagaa
     7801 ctggtcgtgc tggtagagaa ggtatgtcat ttacttttgt atttggaaaa gaaatgagaa
     7861 agatgaaaga tatagaaaga tatactaaat ctaaactgat aaaacataat ataccaacaa
     7921 taactgatgt tgaagagaaa aaagtaggta ctttctttgc acaagttaaa caaactatag
     7981 aagaaggtca tttaactaaa caattacaat ggttagaagg cttctgcaat gatgaagatt
     8041 atgcaatggt tgatatagct gctgcacttg taaaactatc tctaggtgaa gaaatgaaag
     8101 aagagataat agaagaaaaa cctagaagag agcgtggaga tagaaaaggt ggtactggag
     8161 ctaaagatgg tatgattaga ttatttatca atattggtag aaatcaaaga gtacaagcaa
     8221 aagatatagt tggtgctata gctggagaag ttggaatacc aggtaaagta gtaggtacta
     8281 tagatatata tgataagtat acttttgttg aaatacctaa aaaagatgca aaaacagtaa
     8341 ttgaaaagat gaaagatatt aagataaaag gtaataagat taatatagaa aaagcaaata
//
LOCUS       DAEPXF010000163         8355 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain HB27
            SAMN13376389-rid12479423.denovo.170, whole genome shotgun sequence.
ACCESSION   DAEPXF010000163 DAEPXF010000000
VERSION     DAEPXF010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13376389
            Sequence Read Archive: SRR10518432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8355)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8355)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001069624.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001069624.1
            Genome Coverage       :: 315x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/15/2021 01:54:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,165
            CDSs (total)                      :: 4,076
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 89
            rRNAs                             :: 10, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 10 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 19 of 69
            Pseudo Genes (incomplete)         :: 44 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 10 of 69
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8355
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13376389-rid12479423.denovo.170"
                     /strain="HB27"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="China: Hebei"
                     /collection_date="2017"
     gene            <1..817
                     /locus_tag="KRL67_003089"
     CDS             <1..817
                     /locus_tag="KRL67_003089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBH1523953.1"
                     /translation="LYNCIENSGEIDSYLVDGQSFEENLIKDIIENLKMKNNSDIEGY
                     KQSLKELDEYIAKWVHEIKIPISSLSIITDRLSSIEDSLDIKNQVAKINFLVNSILYS
                     SRSNTMFEDVFINKFNLEKLVKMSIKNNSFLLIKNNVEVSLNELENDVYTDSKCMSYI
                     LDQIINNAIKYSKEVGKIEFNSKKLENGVVLSIKDFGIGIDEEDISRVFDKGFTGKNG
                     RNQLYKSTGMGMYFVKKMIDSLGHEIEVCSENGSYTIFNIYFYDISDYLSLDS"
     gene            1248..2408
                     /locus_tag="KRL67_003090"
     CDS             1248..2408
                     /locus_tag="KRL67_003090"
                     /EC_number="2.7.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HBH1523954.1"
                     /translation="MITMYSVGLMSGTSLDGVDAVLAEISGNGRNTKVKQIEFITLEI
                     PKDIKNEIRKCCIEEESSVDVICSLNFKLGYLFSKAVKSVCHKANFHIANLDFIASHG
                     QTIFHIPRSYNNFVSSTLQIGEPAVIAYETNTKVISNFRVMDIAAGGEGAPLVPYSEF
                     LLYSDNNKNLALQNIGGIGNITMIPKSCNIDDVFAFDTGPGNMIIDGVCQRLFNRKYD
                     KNGYFASKGKINEEMLKDLMSHKYLSQAPPKSTGREVFGQVYLDNMLNKYKHVDKYDL
                     IATVTMFTAKAIYYSYKNFILPKVNVDTLLIGGGGAHNLTLIRYIKELLLEVEVLTQD
                     EYGYSSDAKEALAFVILGNETLNNSFSNVISATGAKNKVILGNITPKPFGGK"
     gene            2410..2799
                     /locus_tag="KRL67_003091"
     CDS             2410..2799
                     /locus_tag="KRL67_003091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH1523955.1"
                     /translation="MSNNKKKKNNNYKNAKSTKSNKNTKKQMEKNQRTDYNIPTISFK
                     SGLILIVSSLVSTVVFPLLLSIFEIDNRFGVVIGNSLITSFAVAYTRYFVETKRGFSL
                     GFFRLYILFALSFAIIGYFWIYKGIHI"
     gene            3063..3374
                     /locus_tag="KRL67_003092"
     CDS             3063..3374
                     /locus_tag="KRL67_003092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBH1523956.1"
                     /translation="MRVLFVCSSGMSSAIAVNALQKEGAKNGIDIDVLAVGTQEFEDE
                     VKNGWDIAMVAPQVRHRFDYLKAFADEAGVPCALIQAQAYSPLGGPKLLKQVQELLSK
                     Y"
     gene            3460..4806
                     /locus_tag="KRL67_003093"
     CDS             3460..4806
                     /locus_tag="KRL67_003093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBH1523957.1"
                     /translation="MLDKFTEFLDTKLSTSMARLAEQRHLRAVRDGIVASLPLIIVGS
                     FFLILAFPPLPDSWGVTQYLKANAVTILLPYRMTFFIISVYSTFGIGYSLAKSYKLDG
                     LTGGLLATATFLLTIVPTAIEGLTPDLLKIVEKSPELLAWYNSIPQGWQMPMANLGGG
                     SMFVAIVTSIIGVEIYRFTDKSKFKITMPEAVPASVTRSFEALTPAALIIILMAIVTY
                     FLGFDWHGFIAKLVTPLVSATDTLPSVIILIFLITFFWSFGIHGVSIIGSLARPLWIQ
                     LLDANTASYAAGQAVPGIAAEPFFQWFIWIGGSGATIGLALLLAFRAKSAYGKSLGKA
                     TVIPAIFNINEPIIFGAPIVLNPILIIPFVVAPIVMATVSWFAMSANLVSRVVVTAPW
                     TLPGPVGAYLATGGDWRASVLNLLCIAISIVIYYPFVKMYDKRLLHEEKVEEQEAY"
     gene            5039..6136
                     /locus_tag="KRL67_003094"
     CDS             5039..6136
                     /locus_tag="KRL67_003094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HBH1523958.1"
                     /translation="MGRLGISIYPEKTTFKEIYDYIDKAAENGFTRIFSCLLSVKDTK
                     ENIIKKFKSINEYAKFKGFEVILDVNPRVFGELEISYKDLTFFKKTKADGIRLDMGFT
                     GSEEALMTFNPQNLKIEINMSNNTNYIDTIMDYQPNKENLIGCHNFYPHRYSGLNLEH
                     FIKCTERFNKYGLRTAVFVTSQNETTFGPWSVTDGLPTIEMHRNLPIDIQVKHIIAMN
                     NINDIIISNCYPTYEELKVLGSMRKDMVTFDVAIEENIREVEKKILFEEIHFNRGDIS
                     DNLIRSTQSRVKYKGYKFELFNTPKIIKRGDIVIESSEYGHYAGELQIALTDMKNSGK
                     SNVVGCIRKEELFILDYIKPWQKFNFKPSTK"
     gene            6235..7149
                     /gene="murQ"
                     /locus_tag="KRL67_003095"
     CDS             6235..7149
                     /gene="murQ"
                     /locus_tag="KRL67_003095"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HBH1523959.1"
                     /translation="MLIKTLENLVTEDRNKNTLQIDKEDTLGIIELINNEDKTVAYAV
                     EEQKESIAKAVDIIVDRMKQGGRLFYIGAGTSGRIGILDATECPPTYGVDFELVQAII
                     AGGNQAIFKAVEGAEDDKELGKQDIIDRGVTSKDVICGIAASGRTPYVIGAMEYAKEL
                     GCAVLSITMNPNSEMSKKADLPINIIVGAEVIMGSTRMKSGTAQKMVCNMLTTASMVK
                     MGKVYSNLMVDVKTSNEKLVERAKRIIMIATNIKYDIAEKFLEEADNSVKLAIFMIKS
                     GLDKDSAKNILDRQEGYISEALKSIETL"
     gene            7259..7954
                     /locus_tag="KRL67_003096"
     CDS             7259..7954
                     /locus_tag="KRL67_003096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA reductase"
                     /protein_id="HBH1523960.1"
                     /translation="MYFLGVDGGGTKTTFTLVDEELNIVGTITKGTCHYNQIGFDNLT
                     KLLITGLEEVCKDAKINVEEITYAFVGLAGYGKIKEVLYALEVATKNAYSHINYTLGN
                     DVEIALAGSLNGEKGINIIAGTGSIAQALDKDGNLHRCGGWGYVLGDEGSAYYIGMAT
                     LKMFTMQSDGRCSKTKLYDLIKRHLNIENDYDIIKYVNDEIQGDRIEIAKFATICLKA
                     VIEGDNTASKIFD"
BASE COUNT         3318 a          866 c         1537 g         2634 t
ORIGIN      
        1 tttatataat tgtatagaaa atagtggtga aatagacagt tatttagttg atggacaatc
       61 ttttgaagaa aatctaataa aagatattat agaaaattta aaaatgaaaa ataatagtga
      121 catagagggt tataaacaat cattaaaaga attagatgag tacatagcta aatgggttca
      181 tgaaataaaa attccaatat cttctttaag tataattaca gatagattaa gcagcataga
      241 agatagtttg gatattaaga atcaggttgc aaagataaat tttttagtaa actctatttt
      301 gtatagtagt agaagcaaca ctatgtttga agatgtattt ataaataaat ttaatttaga
      361 aaaattggta aaaatgtcta taaaaaataa ttcatttttg cttataaaaa acaatgtaga
      421 agttagtttg aatgaattag aaaatgatgt ttatacagat tctaaatgta tgtcatatat
      481 tttagaccaa attattaata atgctataaa gtattcaaag gaagttggta agattgaatt
      541 taattcaaaa aagttagaaa atggagtggt attatctata aaagattttg gtataggtat
      601 agatgaggaa gatatatcta gagtttttga caagggattt acggggaaaa atggaagaaa
      661 tcaattgtat aagtctactg gaatggggat gtattttgta aagaaaatga tagatagttt
      721 agggcatgag attgaggtat gttcggaaaa tggttcatat acaattttta atatctattt
      781 ttatgatata tcggattatt taagcctaga tagttaggtt attggactat aaaatctatc
      841 accatataca aatcgacata tacaataaga ataagtttag ttcttgaaaa tagtgaagat
      901 ttaattagtg cctcaaatga tgctctatct atattataaa ataataatta ttatatatga
      961 aaaacatatc agatagatac ctggtatgtt ttttatttgt aataactatt ttaatatggt
     1021 ttttggagaa ataatatgct ttatttatga aaaaaaatta catgatatta cattgacact
     1081 gaaaatgaat taaggtaaga tttagctaag gtaaaaaacg tgtataacat taactcaatt
     1141 aattttgtaa gagtaaatta tcataattaa agatgaaata agattaattc taaaattatt
     1201 aaacttaata tcttgatttg gggaggaata actataaaat taaaaggatg ataactatgt
     1261 atagtgtagg tttaatgtca ggaacttcct tagatggagt tgatgcagta ctagctgaaa
     1321 ttagtgggaa tggtagaaat actaaagtta agcaaataga atttataact ctagaaattc
     1381 ctaaagatat aaaaaacgaa ataagaaagt gctgtataga agaggaatcg agtgttgatg
     1441 taatctgtag cttaaatttt aagcttggat atttatttag taaagctgta aaatcagtgt
     1501 gtcataaagc aaactttcat atagcaaatt tggattttat cgcatctcat ggtcaaacaa
     1561 tctttcatat accaagaagt tataataatt ttgtatcttc aacgcttcaa ataggtgaac
     1621 cagcagttat agcatatgaa acaaatacaa aggtaatatc taattttaga gtaatggata
     1681 tagcagctgg tggagaaggt gctccattag taccatatag tgaattttta ttatatagtg
     1741 ataacaataa aaatttagca cttcaaaata ttggtggaat aggaaatatt actatgatac
     1801 ctaaatcatg taacatagat gatgtatttg catttgatac aggaccagga aatatgataa
     1861 tagatggagt atgtcaaaga ttatttaata gaaagtatga taaaaatgga tactttgcat
     1921 caaaaggtaa aattaatgaa gaaatgttaa aagatttgat gagtcataag tatcttagtc
     1981 aagcgccacc taaaagcact ggaagagagg tatttggaca agtctatttg gataacatgc
     2041 ttaacaaata taaacatgta gataaatatg atttgatagc aacggtaact atgtttacgg
     2101 caaaagctat atattatagt tataaaaact tcatacttcc aaaggttaat gtagatactt
     2161 tattaattgg aggtggagga gctcataatc taactttaat aagatatata aaagagttat
     2221 tattagaggt tgaagtatta actcaagatg aatatgggta ttcttctgat gcaaaggagg
     2281 ctttggcatt tgtaatcttg ggcaatgaaa cattaaataa ttctttttct aatgtaatat
     2341 ctgcaacagg ggctaaaaat aaagttattt tagggaatat aacacctaaa ccttttggag
     2401 gtaaatgaca tgtctaataa taaaaaaaag aaaaataata attataaaaa tgcaaaaagt
     2461 acaaaaagta ataaaaatac taagaaacaa atggagaaaa atcaaagaac agattataat
     2521 attccaacta taagttttaa aagtggatta atattaatag tttcatctct agtaagtact
     2581 gtagtttttc ctttgttatt atctatattt gaaatagata atagatttgg agttgttata
     2641 ggaaattcgt taatcacatc ttttgcagtt gcatatacaa gatactttgt tgaaactaag
     2701 agaggttttt ctttaggatt ttttagactt tatattttat ttgctttatc atttgcaata
     2761 ataggttact tctggatata taaaggaata catatttaaa aaagtatgga gactatttta
     2821 tgttaataat aatatagtct tttttctttt ttgtaagaaa agatatgagt attttaagta
     2881 gtgtgtaact ttagcaaaaa gaatgactag gtaaataaag caattaaagc aagatataaa
     2941 tatatgaaat aaaattacaa ttaaaattgt aaattatgaa aaaatatata gaaacgtatt
     3001 cctgtaatca aaaatacatg ttataatgat tgaaaagaaa taaaaaaaca gggggaatca
     3061 atatgagagt attatttgtt tgttcatcag ggatgtcaag tgctatagca gttaatgcat
     3121 tacaaaaaga aggagcaaaa aatggaatag atatagatgt tttggctgtt ggtactcaag
     3181 agtttgagga tgaagttaaa aatggatggg atattgctat ggtagctcca caagtcagac
     3241 atagatttga ctatttaaaa gcttttgctg atgaggctgg agtaccatgt gcattaatac
     3301 aagcgcaagc ttatagtcca ttaggaggac caaagctatt aaaacaagtt caagagctgt
     3361 tgagtaaata ttagttgcag tattgttagg ttagaagaaa gttaatttta taaaaatttt
     3421 aatgataata aaaataaata ttaattaagg agagaaaaga tgctagataa atttacagaa
     3481 tttttagaca ctaaattatc aacttctatg gccagattgg cagaacaacg tcacttaaga
     3541 gctgtaagag atggaatcgt agcttcatta ccacttataa tagttgggtc attttttttg
     3601 atattagcat ttcctccact accagattca tggggagtga ctcagtattt aaaggcaaat
     3661 gcagttacaa tattactacc atatagaatg acatttttta ttatatcagt atattctaca
     3721 tttggcatag gttatagttt ggcaaagtca tataagttag atggattgac aggaggttta
     3781 ttagctacag ctacattcct tttgacaata gttcctactg caatagaggg tcttacccct
     3841 gatttattaa agatagttga aaagagtcca gaacttttag cttggtataa ttcaattcct
     3901 caaggatggc aaatgccaat ggctaatctt ggtggaggtt caatgtttgt tgctattgta
     3961 acatctataa taggtgttga gatttataga tttactgaca aatctaagtt caagataacc
     4021 atgccagaag cagttccagc atcagtcaca aggtcttttg aagctctgac accagccgca
     4081 ttaataataa ttttaatggc tattgttact tatttcctag ggtttgattg gcatggcttc
     4141 atagctaaat tagtaactcc attagtttca gccactgaca cattaccatc agtaattata
     4201 ctaatatttt taataacgtt tttttggtca tttggaatac acggtgtatc gataataggc
     4261 tcattggcaa gacctttatg gatacaatta ttagatgcta atacagcttc atatgctgca
     4321 ggtcaagcag ttccaggtat tgctgctgaa ccattttttc aatggtttat atggattgga
     4381 ggttctggag ctacaatagg tttagcttta cttcttgcat ttagagcaaa atcagcttat
     4441 ggaaaatctt taggaaaagc aactgtaata ccagcaatat tcaatataaa tgaaccaata
     4501 atatttggag caccaatagt attaaaccca atattgataa taccatttgt tgtagcacca
     4561 atagtaatgg caacagtttc gtggtttgct atgagtgcta atttagtatc tagagtagta
     4621 gttactgctc cttggacatt accaggacca gtaggggcat atttagccac tggtggggat
     4681 tggagagctt cagttcttaa cttattatgt atagctatat caatagtaat ttactatcca
     4741 tttgtcaaga tgtacgataa gaggttactt catgaagaaa aagttgaaga gcaagaagct
     4801 tattaattag aaactatatt ggaaattaaa aactatatta gattagtata aatttcaaag
     4861 tataacagtt atgaaataag taacgaagat attttgttga ttgtttataa aaaaagaaaa
     4921 ggtaaacttt tcttttttta taaatatata tgaaactttt tttcaccaaa tttttaaaaa
     4981 agtttcgtgt aattcaaaat aaatttaaac acatataggg tataaggaga tgacacagat
     5041 gggaagatta ggaatatcaa tttatccaga aaaaacaaca tttaaggaaa tatatgatta
     5101 tatagataaa gctgctgaaa atggatttac acgcatattt tcatgtttat tatcagttaa
     5161 agatactaaa gaaaatataa ttaaaaaatt taaaagtata aatgaatacg ctaagtttaa
     5221 aggttttgag gtaatacttg atgtaaatcc cagagtattt ggtgaattag aaataagcta
     5281 caaagatttg acttttttta aaaagacgaa ggcagacgga ataaggcttg atatgggatt
     5341 tacaggttct gaagaggcat taatgacttt taatcctcaa aatcttaaaa tagaaatcaa
     5401 tatgagtaat aatactaatt atatagatac aataatggac taccaaccaa ataaagaaaa
     5461 tttgatagga tgtcataatt tttatccaca tagatatagt ggattaaatt tagaacactt
     5521 tataaaatgt acagaaagat ttaataagta tggattaaga acagcagtct ttgtaacaag
     5581 tcaaaatgaa accacatttg gaccatggtc tgtaactgat gggcttccaa caattgagat
     5641 gcacagaaat ctacctatag atattcaagt aaagcatata atagctatga ataatataaa
     5701 tgatataata atatcaaatt gttatccaac atatgaggag ctaaaggtat taggttctat
     5761 gagaaaagat atggttacat ttgatgtagc aatagaggaa aatatacgag aagttgagaa
     5821 gaagatactc tttgaagaaa tacattttaa tagaggggac atatctgaca atctgataag
     5881 gtctactcaa tctcgtgtta aatataaagg atacaaattc gaactattta atactcctaa
     5941 aataataaaa agaggagata tagtaattga aagtagtgaa tatggtcatt atgcagggga
     6001 gttacaaata gctttgactg atatgaaaaa cagtggtaaa tcgaatgtag taggatgtat
     6061 aagaaaagaa gaattattta tattagatta tattaaacca tggcaaaagt ttaattttaa
     6121 gccttcaaca aaataattta ttatgaaaat attaatttat tatgtagata taattatgaa
     6181 ggttggaatt aaaatatgtt taaaaattta atattaagaa aatgaggaaa tgatatgtta
     6241 ataaaaactt tggaaaactt agttacagaa gatagaaata agaatacatt gcaaatagat
     6301 aaagaagata cactaggtat aatagaatta ataaataacg aagataaaac tgtagcctat
     6361 gctgttgaag aacaaaagga gagtatagca aaagctgttg atataatagt agatagaatg
     6421 aaacaaggag gtagattatt ctatatagga gcaggtacta gtgggagaat agggatacta
     6481 gatgccacag aatgtcctcc aacttatgga gtggattttg agcttgttca agctataata
     6541 gctggtggaa atcaagcaat atttaaagca gtagaaggtg ctgaagatga taaagaactt
     6601 ggaaagcaag atataataga tagaggagtt acatctaagg atgttatatg tggtatagct
     6661 gcatcaggaa gaactcctta tgttataggt gcaatggagt atgctaagga attggggtgt
     6721 gcagtactat caataactat gaatccgaat agtgaaatga gtaaaaaagc agatttacca
     6781 ataaacatta tagtaggggc agaagtaata atgggttcaa ctagaatgaa aagtggcaca
     6841 gctcaaaaaa tggtatgtaa tatgttaaca acagcttcta tggttaagat ggggaaagta
     6901 tattcaaact taatggtaga tgttaagaca tcaaatgaaa agttggttga aagagcaaaa
     6961 agaataataa tgatagctac aaatataaaa tatgacattg cagaaaaatt cttagaagaa
     7021 gcagataata gcgttaaatt ggctatattt atgataaaaa gtggtttaga taaggatagt
     7081 gcaaaaaata tactagatag acaagaaggt tatataagtg aagctttaaa atcaatagaa
     7141 acattgtaaa ataaataata atactgataa cctttcaata ttaaaagaac agcattggaa
     7201 ggttattttt agatattaag tattgaaaga attattaaag gtattgtagg aggatagtat
     7261 gtatttttta ggggtagatg gtggaggaac gaaaacaaca tttacattag tagatgaaga
     7321 attaaatata gtaggaacca taaccaaagg aacttgtcat tataatcaaa taggatttga
     7381 taatctaacg aaattattaa taactgggtt ggaagaagtt tgtaaagatg ctaagataaa
     7441 tgtagaagaa ataacgtatg catttgttgg acttgctgga tatggaaaaa taaaagaagt
     7501 attgtatgca ttagaagtag caacaaaaaa tgcatatagc catataaatt atactctagg
     7561 caatgatgta gaaatagcat tagcaggttc tttaaatggg gaaaaaggta taaatataat
     7621 tgcaggtact ggttcaatag cacaggcttt agataaagat gggaatcttc acagatgtgg
     7681 aggatggggc tatgtattag gagatgaagg ttcagcgtat tatataggaa tggccaccct
     7741 aaaaatgttc acaatgcagt ctgatggaag gtgttcaaaa acaaaattat atgatttaat
     7801 aaaaagacat cttaatatag agaatgatta tgacataata aaatatgtaa atgatgaaat
     7861 acaaggagat agaatagaaa tagcaaagtt tgctacgata tgtttaaaag cagttattga
     7921 aggtgataat actgctagta aaatatttga ttaaaaaata gctacgcaca cttgtgactt
     7981 tagtcatgag ttagtagcta aaatagtcag catataggga aacctatatg tagagatagg
     8041 atagaaccta tccaatacta cttgaattgc taaaaacccc taaagctaac caaactaaaa
     8101 cataaggatg aaataaacct aagtgtgaaa gttacgaaag tagaaaaaat tggttagatg
     8161 gtataaggtt aaaccctaag tactttaaaa tgggcaatca gcaggtaagc tctgaatagg
     8221 agaaacttca acgactattc ctcttgaggg aagtacacta caagctattg gtagtggaag
     8281 tgggtagacc ttaacggata atgccgaagg ataagatata gtctgtgctt acatgaaagt
     8341 gtaagaagtt cataa
//
LOCUS       DAEPXF010000164         8343 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain HB27
            SAMN13376389-rid12479423.denovo.171, whole genome shotgun sequence.
ACCESSION   DAEPXF010000164 DAEPXF010000000
VERSION     DAEPXF010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13376389
            Sequence Read Archive: SRR10518432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8343)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8343)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001069624.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001069624.1
            Genome Coverage       :: 315x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/15/2021 01:54:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,165
            CDSs (total)                      :: 4,076
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 89
            rRNAs                             :: 10, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 10 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 19 of 69
            Pseudo Genes (incomplete)         :: 44 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 10 of 69
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8343
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13376389-rid12479423.denovo.171"
                     /strain="HB27"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="China: Hebei"
                     /collection_date="2017"
     regulatory      16..101
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            225..6146
                     /locus_tag="KRL67_003097"
     CDS             225..6146
                     /locus_tag="KRL67_003097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-binding adhesion protein"
                     /protein_id="HBH1523961.1"
                     /translation="MKKAISCVLAVSMCSAPLNVFAEPILEGKLRAVEKSVTEKLRGN
                     LEVDLNFSLPIKNSESESMTNMQLRLKDDSNNSGIIKLGEKDSGTVNVGTDNIEYTIK
                     KLSASRSEIKNKDENISYYNIVFNNLPVGKYNIEVSGAGFKNKEIKDIDISSYSQRVL
                     LSNRANVDNKNKPLNYELFLMGDVNGDGKVDKSDYNKVLENIDSNKREFDLNRDGKVD
                     IVDLDYVQKNLGQSEDAKSLESIVSTNPIVDTSKVELKGNSDVDINGNVENLFSGENN
                     GVTISSKNDKISEEKPAVMDIALSEALNMEVINIKAPEGTAPAKGIVEVTDKDGNVQQ
                     IPYENKEYMKNLKDEKQQEEVNNEQEEVNQEEVNNKDENNDKQQENIDKQEVNSNTNQ
                     DKNNDKENSSMDKPNSDEDTPKEEGNISKDDNSTTSTYSRSALETYADVQNSDIVINL
                     GKQVAVKKITIKITATTANDRNLAEISKVEFLNNVYKEIPKPEMNIPKINKISTSTAV
                     GNEKVSFYWNNEVNVSAYELILDKIDSKGKVLSTKKLQTSKNTIEVRDIEAYGLYRVS
                     IQSLNGDWSSGYKDATPENLDGVPENVDSNYKPIQFSPDSILEFQVVPDTKPEPPEGI
                     NVKGKFKSLDVSWKNHKQAKDFDLYYKEKDSNGSWIKYNDKLIRGTSSKIEGLKDNTT
                     YEVRMTATNHLGTSGMSKTYIGTTESMDAPTTPNYRLINTPKENGGFGIPTNHIVDVV
                     YPDGYDKSEYSDKNPFDKFNVVDGDFTTHWTFPTYNAAEGTNRGPIITFDKSYTMDTF
                     MATPRFDVSDKTINDFEVRYWDENGQLHNLGDVPFETKTDPVNNNKYVMVRLKEPITA
                     TKIQVSVCSSYSYKSNISELKFYEYDSIEDDVRNLFMDDLQVELKEEVTQEKITELKD
                     RLNTPDKASGEYHPYKTVIDRELKLAQDLYNDRATLTDVTTVNQEINKSGKYKDKNGQ
                     YTVNSNNLGMQNDWQALGVAARAGDEITVYVGSKSGKTPKLIYTQYYGESGAYKSGEI
                     NLKTGKNVIQLSKLHSLDIECGGSLYIRYPEDTPSGGDIKVRVAGATKIPHLNLNGTI
                     NDKSPAGVSESKKKIKAYIEELAKYKDAVKGEPYYPGTNVGNSYGYSEKTGVLNTTDI
                     ESDKVTLNVPATAVYQGISKGNDDLNTQVDRLYNSMLAWEQIMDLVYSERGVFKTQDL
                     DGNGTVDDNEFNLTKNDMAPKSRMNIKYQRMFIGAFMYASGLHVGVEYGSVPGLMNGV
                     PFQIDDSGKATGGNLFGWGIGHEIGHVTDIGKMTYSETSNNVLALLAQTFDDKTHSRL
                     EGSTMYKIYEHVTSNSLGIPSNVFERLGMLWQLHLAYDDDFTGSMLKNNSDADLSNDT
                     FYAKISRKYRALSSSDPINSLPKDQMLVAMASSVVEKDLRDYFKAWGVEITPELNSIM
                     DSKNYEKESRKIQYVNDEARRKILNENIASMAKDTKVSASFSDGIQSGSLVKSNKISI
                     DLDVDKDKDKILGYEIIRSDGNYLDGENGTQVKYRTVGFVDAKGNSKAKFTDNISPLN
                     NRAFTYKVVAYDYHLNPTEEFEVGTVKLSDEGKINKSAWSFITNTVSDGDVRTENDSH
                     GPLQNPEIDNIKDNDASTTYKGKIISKAEWNKDPQKDPDINVEENPYIIVDMKETLPI
                     VGLKYTKPKTEARKFSLKGLFNFRKNTSTTYNPLTNYKIQVSNDKKNWEDVSQGTFEY
                     GQDLVGGGKDQDNEARVTFNKDGKLWTYQARYVKLISNNKSNIEVAELDIIGPPGDNI
                     EIGTMNNADETRTNGIGKLAEDYVYQSDDSSTSEDETGKIPAGSIVITGEYSGNPAFN
                     LPLLIDKNNKTISGEALLFAEVPEKGELGEISKGTWVYYMPKENFDSLSDKVKAELYR
                     YNDLQGDTPVGQRFVSDTMYVPVGAKNYDELKTISLTDSNSKARKATRNSIDISNKKV
                     ITVSNKVKGNIVRDK"
     gene            6357..8237
                     /locus_tag="KRL67_003098"
     CDS             6357..8237
                     /locus_tag="KRL67_003098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp10"
                     /protein_id="HBH1523962.1"
                     /translation="MSKRKSFIRTIAVSTMAVAVTGSATCAYAAPVLQGTKTYEKVNT
                     IDISVDSVENIVYSFQASIKVQGEVEVVDNEQKEKITWSDNIKSQIKSGNANATCRAE
                     YNKSSNTTTLDIYVTSNEDLLDGNRLNIGRISVKKSGSNSNADYKVFGKGTSDKPALK
                     IVTYNNKTVDYENINSDEGLIFTLINESEVKPIGGTGSSKNDPEKYKVEKSEALEYLL
                     NNIRINYSIVSKETQESGSNVILKLGLAQKTTKGRKATINKYVEVTLPKSLEYIVENE
                     LSKPDELPPDNGNGGNNGGGSNSGGSSSGGSSGGGNSSDSTSNVTVKKLKGADRFETA
                     IKISQSGWTKSDTVVIVNGEDKSMVDGLTATPLASIKNSPILLSSNEKLPQKTVEELK
                     RLNPSKVIVIGGNNSMPNSVVEAIKAVNSKISVQRIGGDTRYQTSINIAKEIDKTNNV
                     SKLYIGAGNGEADSLSIASLAGKEKTPIVLTQKDGVDNEAEQFIKSNKVSNIYFIGGI
                     EKISNKAIEQVGKIANKDISKNRVAGQTRQETNAKVIDKFYSQSKLDGVVVANQDKLI
                     DALAVGPLAAKNNSPVILATNTLDKSQESSLKGKNSSKLFEVGGGVASSVVDKIKSLI
                     EK"
BASE COUNT         3545 a          967 c         1557 g         2274 t
ORIGIN      
        1 ggtaagaaag tagtgaaaat taaatggcaa aattagggaa atctaatgac gcaaaactat
       61 agggattaaa gcttttaaag ccatatcagc cagttgctaa aaagagtaaa gtctttttat
      121 tttataatta agattctctt tgagtgtctt tttttattgt ctgaaactta tagtatttaa
      181 attcatgaaa aattagatat ttattaaggg aggattgttg tttcatgaaa aaggcaatat
      241 cttgtgtact agcagtatct atgtgtagtg caccactaaa cgtttttgca gaacctatat
      301 tagaggggaa gctaagggca gttgaaaaat cagttacaga aaagttaagg gggaatcttg
      361 aagtagattt aaatttttca ctgccaataa aaaatagcga gtcagaaagt atgactaata
      421 tgcagttacg attaaaagat gatagtaaca atagtggaat aataaagctt ggagaaaaag
      481 attcaggaac agtaaatgtt gggacagata atatagaata tactataaaa aaattatctg
      541 caagtagaag tgaaataaaa aataaagatg agaatatatc atattataat attgtattta
      601 ataatttacc tgttggaaaa tacaatatag aagtctctgg agctggattt aagaacaaag
      661 aaattaaaga cattgatata tcaagttatt cacaaagagt cttattaagc aatagagcaa
      721 atgtagataa taaaaataaa cctttaaatt atgaattatt cttaatgggt gatgtaaatg
      781 gagacggtaa agtagataag agtgattata ataaggtttt agagaatata gattcaaata
      841 aaagagaatt tgatttaaat agagatggaa aggtagatat tgttgactta gattatgtac
      901 agaaaaattt aggacaaagt gaagatgcaa aaagcctaga aagtatagta tctaccaatc
      961 ctatagtaga tacaagtaag gttgaattaa aaggaaatag tgatgtagat attaatggta
     1021 atgtagaaaa tttattcagt ggagaaaata atggagttac aatatcttca aaaaatgata
     1081 aaatatctga ggaaaaacca gcagtaatgg atatagcttt atcagaagca cttaatatgg
     1141 aagtcatcaa tataaaagct ccagaaggta ctgctccagc aaaaggaata gttgaggtta
     1201 ctgacaaaga tggtaatgtt caacagattc cttatgaaaa caaagaatat atgaaaaact
     1261 taaaagatga gaagcaacaa gaggaagtaa ataacgaaca agaagaagta aatcaagaag
     1321 aagtaaataa taaagatgaa aataatgata aacaacagga aaatatcgat aaacaagaag
     1381 taaactctaa taccaatcaa gataaaaata atgataaaga aaattcatca atggataaac
     1441 ctaattcaga tgaagatact ccaaaagaag agggtaatat cagtaaagat gataactcta
     1501 caacatctac atatagtagg tcagcattag aaacatatgc agatgttcaa aatagtgata
     1561 tagtaataaa tcttggtaaa caagtagcag taaaaaagat aactataaaa ataacagcta
     1621 caacagcaaa cgatagaaat cttgcagaaa tatcaaaagt tgaattttta aacaatgtat
     1681 ataaagaaat accaaagcca gagatgaaca tacctaaaat aaataaaata tcaactagca
     1741 ctgctgttgg aaatgaaaaa gtatcttttt attggaataa tgaagtaaat gtatcagcat
     1801 acgaacttat actagataaa attgatagta agggaaaagt attatctact aaaaaattac
     1861 aaactagtaa aaatacaata gaagttagag atatagaagc atatggacta tatagagtta
     1921 gcatacaatc tttaaatgga gattggtcta gtggttataa agatgcaaca ccagaaaact
     1981 tagatggagt accagaaaat gtagattcaa actataaacc tatacaattt agcccagata
     2041 gtatattaga atttcaagtt gttccagaca caaaaccaga accaccagaa ggtataaatg
     2101 ttaaaggtaa atttaaatct cttgatgtaa gctggaaaaa tcataaacaa gctaaagact
     2161 ttgacttgta ctataaagaa aaagattcta atgggtcttg gataaaatac aatgataagc
     2221 ttattcgtgg gacaagttca aagatagaag gtcttaagga taatacaaca tatgaagtta
     2281 gaatgacagc tactaaccac ttgggaacta gtggaatgtc caaaacatat ataggtacaa
     2341 ctgaaagcat ggatgcacca acaacaccta actatagatt aattaatact cctaaagaga
     2401 atggtggatt tggaatacca acaaatcaca ttgtagatgt tgtttatcca gatggatatg
     2461 acaaaagtga atatagtgat aaaaatccat ttgataaatt caatgtagta gatggggact
     2521 ttacaacaca ttggacattc ccaacgtata atgcagctga gggaacgaat agaggaccaa
     2581 taataacttt tgataaatcc tatactatgg atacttttat ggctacacct cgttttgatg
     2641 tttctgacaa gacaataaat gactttgagg tacgatattg ggatgaaaat ggtcaattac
     2701 acaatctggg ggatgttcct tttgaaacaa agacagaccc tgtaaataat aataagtatg
     2761 taatggttag attaaaagaa ccaataactg ctactaaaat acaagtaagt gtttgctcat
     2821 cctattcata taagtctaat atatcagaac ttaagtttta tgagtatgac agtatagaag
     2881 atgatgtaag aaatctattt atggatgacc tacaagttga attaaaagaa gaggtaactc
     2941 aagaaaaaat aactgaatta aaagatagat taaatactcc agataaagct agtggagaat
     3001 atcatccata taaaacagtt attgatagag aattaaaatt agctcaagac ttatataatg
     3061 atagagccac acttactgat gtaactactg taaatcaaga aataaataaa tcaggaaaat
     3121 ataaagataa aaacggacag tatactgtaa attccaataa tttaggtatg caaaatgact
     3181 ggcaggcatt aggagtagct gctagagcag gggatgaaat cacagtatat gttggaagta
     3241 aaagtggaaa aactcctaag ttgatatata cacagtacta tggagaatct ggtgcatata
     3301 agagtggaga aataaatctt aaaactggaa aaaatgtaat tcaattaagc aaacttcata
     3361 gtttagacat agaatgtgga ggaagtttat acataagata ccctgaagat actccaagtg
     3421 gaggagatat aaaagtacgt gtagcaggag ctactaagat accacatctt aatctaaatg
     3481 gaacaataaa tgataagagt cctgcgggag tttctgaatc taagaaaaag ataaaagcat
     3541 atatagaaga attagctaaa tataaagatg ctgtaaaagg agaaccatat tatccaggaa
     3601 caaatgttgg taattcatat ggatattcag aaaaaactgg tgtgttaaac actacagata
     3661 ttgaaagcga taaagtaaca ttaaatgttc ctgcaacagc tgtatatcaa gggatatcaa
     3721 aaggaaatga tgatttaaat acacaagtag atagattata taatagtatg cttgcatggg
     3781 aacaaataat ggatttagtt tactcagaaa gaggagtatt taaaacacaa gatttagatg
     3841 gtaatggaac tgtagatgat aatgaattta acttaactaa aaatgatatg gctccaaaat
     3901 ctcgtatgaa cataaaatac caaagaatgt ttattggagc atttatgtat gcttctggat
     3961 tacatgtagg agtagaatat ggttcagttc cagggcttat gaatggagta cctttccaaa
     4021 tagatgatag tggaaaagct acaggaggaa acttatttgg gtggggaata ggtcatgaga
     4081 taggacatgt tacagatatt ggaaagatga catatagtga aactagtaac aatgtacttg
     4141 ctttacttgc tcagaccttt gatgataaga cacattcaag acttgaaggt tcaacaatgt
     4201 ataagatata tgagcatgta acttcaaata gcttaggtat accaagcaat gtatttgaaa
     4261 ggcttggaat gttatggcaa ttacatctag cttatgatga tgattttacg ggttcaatgt
     4321 taaagaataa ctcagatgct gatttaagta atgatacatt ctatgcaaag atttcaagaa
     4381 aatacagagc actttcaagt agtgacccaa ttaatagttt gccaaaagac caaatgctag
     4441 tagcaatggc atcaagtgtt gttgaaaaag atttaagaga ttactttaag gcatggggag
     4501 tagaaattac accagaatta aacagtataa tggatagtaa gaactacgaa aaagaatcaa
     4561 gaaagataca atatgtaaat gacgaagcta gaagaaagat tttaaatgaa aatatagcta
     4621 gtatggcaaa agatactaaa gtaagtgcta gtttttctga tggaatacag agtggttctc
     4681 tagttaaatc taataaaata tctatagacc tagatgtaga taaggataaa gataagatac
     4741 taggatacga aataataaga agtgatggaa attatttaga tggtgaaaat ggaacacagg
     4801 taaaatacag aacagttgga tttgtggatg caaaaggaaa tagtaaagct aaatttactg
     4861 ataatatatc tcctcttaat aacagagcat ttacatataa ggtagttgca tatgattatc
     4921 acctaaatcc aacagaagaa tttgaagttg gtactgtaaa attatcagat gaagggaaaa
     4981 taaataaatc agcatggtca ttcataacaa atactgtttc tgacggtgat gttagaactg
     5041 aaaatgactc acatggacca ttacaaaatc ctgaaattga taatataaaa gataatgatg
     5101 caagtactac atataaagga aagataataa gtaaagcaga gtggaacaaa gaccctcaaa
     5161 aagaccctga tataaatgta gaagaaaatc catatataat agtagacatg aaagaaacat
     5221 tacctatagt aggactaaaa tatacaaaac caaagacaga agctaggaag ttctcattaa
     5281 aaggattatt taactttaga aagaatacta gcacaacata caatccttta actaattaca
     5341 agatacaagt aagtaatgat aaaaagaact gggaagatgt aagtcaagga acttttgaat
     5401 atggacaaga tttagttggt ggaggaaaag accaagataa tgaagctaga gttacattta
     5461 ataaagatgg taaactttgg acataccaag ctagatatgt aaaactaatc tcaaataata
     5521 agtctaatat agaagttgca gaattagata taataggacc tccaggtgat aatatagaaa
     5581 ttggcacaat gaataatgca gacgaaactc gtactaatgg aattggaaaa ttagcagaag
     5641 attatgtata tcaaagtgat gactctagca caagtgaaga tgaaacaggt aagattccag
     5701 caggttcaat agttattaca ggtgaataca gtggaaatcc agcatttaat ttaccactac
     5761 ttatagataa aaacaataag actatatctg gagaagcact tttatttgca gaagttccag
     5821 aaaaaggtga attaggtgaa attagtaaag gtacatgggt ttactatatg ccaaaagaaa
     5881 actttgattc tttaagtgat aaggttaagg ctgaactata tagatacaat gaccttcaag
     5941 gagatactcc agtaggtcaa agatttgtaa gtgatacaat gtatgtacct gtaggagcta
     6001 agaattatga tgagttaaag acaataagtc ttactgatag taatagtaaa gctagaaaag
     6061 ctacaagaaa ttctattgat atcagtaata agaaggttat aactgtaagc aataaagtaa
     6121 agggtaatat tgttagagat aaatagtagg tggaactcta aaaacgtgaa aatactaata
     6181 ctaacattgt atatttaata tgattaggta ctagaatagt ttttaaatag tagatattaa
     6241 aagtaaagta tataaatgta actataagtt tagggttaat ttataaatta aatataaaag
     6301 ctaggtggat aaataaattt atccacttag ttaatgatag ataaggacgg gtgttaatga
     6361 gtaaaagaaa atcttttata agaactatag cagtatcaac tatggcagta gctgtaactg
     6421 gtagtgctac atgtgcatat gcagctccag ttttacaagg aacaaaaacg tatgagaaag
     6481 taaatactat agatatatct gtggactcag tagaaaacat agtatattca tttcaagcca
     6541 gtataaaagt acaaggtgag gttgaggtag tagataatga gcagaaagaa aagattactt
     6601 ggtctgataa tataaaatct cagataaaaa gtggaaatgc aaatgctact tgtagagcag
     6661 aatacaacaa atcaagtaat acaactacat tagatatata tgttacatca aatgaagatt
     6721 tactagacgg aaatagattg aatataggaa gaatatctgt aaaaaaatct ggcagtaact
     6781 caaatgccga ctataaagtt tttggaaaag gaacaagcga taaaccagcc ttaaaaattg
     6841 taacatataa caacaaaaca gtggattatg agaatataaa ctctgatgaa ggattaatat
     6901 ttacccttat taatgagagt gaagtaaaac cgattggtgg aactggttct agtaaaaatg
     6961 accctgaaaa gtataaggta gagaaaagtg aagcacttga atatttatta aacaatatta
     7021 ggataaacta ttctattgtt agtaaagaaa cacaagaaag tggttcaaat gtaatattaa
     7081 aacttggatt agcacaaaaa acaactaagg gaagaaaagc tacaataaac aaatatgttg
     7141 aagttacact tccaaaaagt ttagaataca tagttgaaaa tgaattatca aagccggatg
     7201 aattaccacc agataatggc aatggtggaa ataatggtgg aggaagtaat tcaggcggtt
     7261 cgtctagtgg tggaagctct ggaggaggaa attcaagtga ttctacatct aatgtcacag
     7321 tcaaaaaatt aaaaggtgct gatagatttg agacagctat caaaatatct caaagtggtt
     7381 ggacaaaatc agatacagtt gtaatagtaa atggagaaga taaaagtatg gtggatgggc
     7441 ttacagctac accacttgct agtataaaaa actcaccgat tcttttatca tcaaatgaaa
     7501 agcttcctca aaaaactgta gaagaattga aaaggctaaa tccatcaaaa gtaattgtga
     7561 taggtggaaa caactcaatg ccaaattcag ttgttgaagc tatcaaagct gtaaattcaa
     7621 aaatatcagt acaaagaata ggtggagata caagatatca aacttctatt aatatagcaa
     7681 aagaaataga taagacgaat aatgtaagca agttgtatat aggagcagga aatggagaag
     7741 ccgattcatt atcaatagca tcactggctg gtaaagaaaa aactccaata gtacttacac
     7801 aaaaagatgg tgtagataat gaagctgaac aatttattaa atcaaataag gtgtcaaata
     7861 tatattttat tggtggaata gaaaaaatat ctaacaaggc tattgaacaa gttggcaaaa
     7921 tagcaaacaa agatatatca aaaaataggg tggcaggtca aactagacaa gagacgaatg
     7981 ccaaagtaat agataaattc tactctcaat ctaaactaga tggagttgta gttgcaaatc
     8041 aagacaaact tatagatgct ttagcagttg gaccattagc agctaaaaac aattcaccag
     8101 ttatacttgc tacaaatacg ttagataaat cacaagaatc atcattaaaa ggtaaaaatt
     8161 catcaaagtt gtttgaagta ggtggaggag tagcctcatc tgtagtagat aagattaaga
     8221 gtttaattga aaaataaata ttaagtaaat agaaaataag aatgtattta gatataaagt
     8281 tatatattta aatgcattct tattttattt tcattatctt aacaatttaa taatattgag
     8341 gtt
//
LOCUS       DAEPXF010000165         8306 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain HB27
            SAMN13376389-rid12479423.denovo.172, whole genome shotgun sequence.
ACCESSION   DAEPXF010000165 DAEPXF010000000
VERSION     DAEPXF010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13376389
            Sequence Read Archive: SRR10518432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8306)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8306)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001069624.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001069624.1
            Genome Coverage       :: 315x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/15/2021 01:54:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,165
            CDSs (total)                      :: 4,076
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 89
            rRNAs                             :: 10, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 10 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 19 of 69
            Pseudo Genes (incomplete)         :: 44 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 10 of 69
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8306
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13376389-rid12479423.denovo.172"
                     /strain="HB27"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="China: Hebei"
                     /collection_date="2017"
     gene            95..1081
                     /locus_tag="KRL67_003099"
     CDS             95..1081
                     /locus_tag="KRL67_003099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBH1523963.1"
                     /translation="MKKITINDIANLAGVSKSTVSRYLNNKDISDSTKEKIKTIIDEY
                     GYEPNAFAQSLRAKKTYFIGIITPCLDSFVKSKIMMAIDEELKELKYTSLIINTSRKI
                     RSEIDSISKLASLKVDGIILIGTEITKEHKNVIEKLDIPIVVVGQKVDGINSIVNDDY
                     GAGYKMGQYIANKGYKNIVYLGVDESDISVGLNRKNGVLNGLKDKGYDAKVFYTDFDQ
                     ETSIQRSGEMLESENPDIIICATDNIAIATMKEINKRGKNIPRDISVAGFGGYDILSI
                     ISPKLTTIKFENKNAGKVAANTIVNLIQERKEPLLKEIKFELIEGESTINKN"
     gene            1251..1397
                     /locus_tag="KRL67_003100"
                     /pseudo
     CDS             1251..1397
                     /locus_tag="KRL67_003100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423822.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIB"
     gene            1419..2900
                     /locus_tag="KRL67_003101"
     CDS             1419..2900
                     /locus_tag="KRL67_003101"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HBH1523964.1"
                     /translation="MYKEPKYRTILESTKEELNKLRNISLNDKYKPLFHIHPQHGLLN
                     DPNGLAYYNGKYHVFYQWYPYDATHGMKHWAYVSSDDFVNWNREDVALIPIESYESHG
                     VYSGNAIEVDGKLHMYYTGNIKYSTEDRYAYQNLAIMDKDGKMTKYENNPIVSEIPKG
                     YTGHVRDPKVFKRKDKYFMLLGAQTSDKKGVIIVYESKNSIDWNFKGELNIKNIDEDF
                     GYMWECPDYINIDGKDVLIFSPQGVEPKGFDYQNIYNVVYAIGNMDLDNLTFQIDIMK
                     EMEKGFDFYAPQTFTKESQIILFAWAGMGEVLYPTDKNKWAHCLTVPRKLNIKNNKLL
                     QMPVDELIKLRYDETIGQNTIKNNINIIENDENVYELNINIKNIDSNKFGLELFSSQD
                     EGVKLEFNKVGNTVTLDRSNFKKVFGVEYGTNRKEYINIDENTNIKVLADRSILEIFI
                     NDGEVVFTSRIFAKENSNQIRVYSDKIVEYKYTKFKLKQGIEL"
     gene            2926..3897
                     /locus_tag="KRL67_003102"
     CDS             2926..3897
                     /locus_tag="KRL67_003102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase"
                     /protein_id="HBH1523965.1"
                     /translation="MKKVISIGEALIDFIPNQNGCKLKNVSGFRRVAGGAPANVSAVV
                     AKLGGKSSFISKLGKDAFGDYIIDVLNEVNVNTDYVLRTSKANTGLAFVSLKEDGNRD
                     FSFYRNPSADMLLEADEVKKEWFNDCHSLHFCSVDLIDSPMKLAHKKAIEYALESNSI
                     ISFDPNIRLPLWDSEQSCKNAISEFLPFAHIVKISDEELEFITGENDIEKSLHKLFVG
                     NVELVLYTKGKDGVDAYTKKVKGMCKGVKVNAIDTTGAGDSYIGSFLYTLLYKQITLN
                     DIKEMKQETLNEYLEFSNYYAAHSTTIKGAISSYATKEEITEFIKNL"
     gene            complement(4153..4755)
                     /locus_tag="KRL67_003103"
     CDS             complement(4153..4755)
                     /locus_tag="KRL67_003103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HBH1523966.1"
                     /translation="MEVCILMGSPKKKGNTAAILKPFIEELENYNSNIEVIWLYDCKI
                     EPCIACKKCQDNLFDFGCYCKDDVQKIFKKILMCDLIVFATPIYSWYCTSPMKALLDR
                     LVYGMNKYYGNEKGPALWAGKPVALITTCGYRPGKGADIWENGMKRYCKHSQLKYIGM
                     LVERDLGKPNFMNKEKEEHSRQFAKQIYYILDNSIKVDNC"
     gene            5036..6247
                     /locus_tag="KRL67_003104"
     CDS             5036..6247
                     /locus_tag="KRL67_003104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-dependent decarboxylase"
                     /protein_id="HBH1523967.1"
                     /translation="MYSNEDYKFIEENAPCYIYDETNIIEKCKELKNNILGFDFLYSI
                     KTNPYNKIVKSIAEQGFGADASSTNEVMISSKCGVSKDDIYYSSPGKTEIDIETVLDK
                     CIIIADSITEIELINKISKKHNQKVKIGIRINPDFTMDDDSAKSSKFGIDIEDMSSVF
                     KMLSMCDYVSVVGIHIHIKSQVLDFEKLGHYYEKCFFIALNLNKSKYINIEFINFGSG
                     LGVAYDNLRENPLNLKELSKLTQKIAFTNSNTLKSRLLIESGRFIVCKSGNYYVKIVD
                     IKVSRGKKYLIVKNGLNGFMRPIISNLIENITCGVELEGQEPLYTSNHAFSLSVLNNL
                     KEEEYVNVVGNLCTSLDVIANNVKLKKANIGDLISISNAGSYSYSLSPLLFSSYEFPK
                     QYYKRKDGYIE"
     gene            6315..6512
                     /locus_tag="KRL67_003105"
     CDS             6315..6512
                     /locus_tag="KRL67_003105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter"
                     /protein_id="HBH1523968.1"
                     /translation="MELWKRNLFVCWIGMFFSSIGMSQIAPILPLYIKQLGVTDVSLI
                     QQYSGIIFGCTFVVAAFFSPI"
     gene            6546..7517
                     /locus_tag="KRL67_003106"
     CDS             6546..7517
                     /locus_tag="KRL67_003106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBH1523969.1"
                     /translation="MLLRASIGMGIVIFTMAFVQNVYQLLGLRILQGVFTGYATACTT
                     LIATQTDKNHSGWALGTLATASTTGSLIGPTVGGYIESILGLKSTFIITGGLLFVSFI
                     ISILFVVDNFKPKEIKESISIPKEQLNVLPNKFLIASIFVTTFITQLALYSIEPIVTI
                     YISQLTNFASNVALIAGLTFSASGLANLLAAQKLGKMSDKIGPQKVLLISLLWAGIIF
                     IPQAFVKTAWQLMLLRFLLGLSVAGLNPSVNSLLKKIAPEEYVGKIFGYNASAQYIGC
                     SSGAFLGGQISAHLGIRTVFFSTSLLLFMNALWMYKFTGLFIKDKTK"
     gene            7704..8081
                     /locus_tag="KRL67_003107"
     CDS             7704..8081
                     /locus_tag="KRL67_003107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HBH1523970.1"
                     /translation="MTISKIPQAELKVMKFIWEKNDTVTSKETIEAMERKYGWKQTTT
                     LTLLSRLVKKHFLSAEKIDRYTHYTIVVGHKEYLSVETKDFLSNIHDNSLQSLISALH
                     DDEVVDKDMLDLFESHFKNLEEE"
BASE COUNT         3374 a          875 c         1338 g         2719 t
ORIGIN      
        1 atataaaaag tattgactgg acaaataact atattttatt ttaaatatat aatataatgt
       61 aataaaatat atcaaatgta gaaggtgata acctatgaaa aaaataacaa taaatgatat
      121 tgctaacttg gcaggagtat ctaaaagtac tgtatcaaga tatctaaata ataaagatat
      181 cagtgattct actaaagaga aaataaagac aataatagat gaatatggat atgagccgaa
      241 tgcatttgct caaagtttga gagctaaaaa aacatacttt attggtatta taacaccgtg
      301 tttagattct tttgtaaaat ctaaaattat gatggcaata gatgaagaac tcaaagaatt
      361 aaaatataca tcactaatta ttaatactag tagaaaaata aggtcagaaa tagatagtat
      421 atcaaaatta gctagtctga aagttgatgg aataatacta attggtaccg aaataactaa
      481 agaacataag aatgtaattg aaaagttaga tataccaata gttgtagttg gacaaaaagt
      541 tgatggtatc aatagcatag taaatgatga ttatggagca ggatataaaa tgggtcaata
      601 tattgctaat aaaggttaca aaaacattgt atatttaggt gttgatgaaa gtgatatatc
      661 agttggttta aatagaaaaa atggagtact taatgggtta aaagataaag ggtatgatgc
      721 aaaagtattt tatactgact ttgaccaaga aacgtctata caaagaagtg gagaaatgct
      781 agaaagtgaa aatccagata taataatatg tgcaactgat aatatagcaa tagcaacaat
      841 gaaagaaatc aataagagag gtaaaaatat accacgagat atatctgtag ctggatttgg
      901 tggatatgac atattgtcta ttataagccc taaacttaca acaataaagt ttgaaaataa
      961 aaatgcaggt aaagtagcag caaatacgat agtaaactta atacaagaaa gaaaagaacc
     1021 actattaaag gagattaaat ttgaattaat agaaggtgaa agtacaataa ataaaaatta
     1081 agatacacat tacatatatt agtaatgtgt atttttttac taaaaaaact ataattctaa
     1141 aaattaggaa aacttttttg aaagaaatat tgcatctaaa ataaatttat gataatcttt
     1201 aattaaaccg gtttcgaaac cggtttaatt aaaaccgaga gaatatcaaa atgagtgcaa
     1261 aaataaatga ttataacaaa gtatcaaagc aattattaga gtacatagaa aataaacaaa
     1321 atataaaagg tgtagcacat tgtgtaacga gattaagaat tgttttagat agtaataata
     1381 aagcaaatat aaaattataa aagaaaagga ataaaaatat gtacaaagag cctaaatata
     1441 gaaccatatt agaatcgaca aaagaagaat taaataaatt aagaaatata tctttaaatg
     1501 ataaatataa accactattt catatacatc cacaacatgg actacttaat gacccaaatg
     1561 gattagcata ttataatggt aagtaccatg tattttatca atggtatcca tatgatgcaa
     1621 ctcatggaat gaagcattgg gcttatgtaa gttcagatga ttttgtaaat tggaatagag
     1681 aagatgtagc attaatacca atcgaaagtt atgaatctca tggagtatat tcaggtaatg
     1741 cgatagaggt agatggaaaa ttgcatatgt attatacagg gaatataaaa tatagtacag
     1801 aagatagata tgcataccaa aacttagcta taatggacaa ggatggtaaa atgactaaat
     1861 atgaaaataa tccaatagta agtgaaatac caaaaggata tacaggacat gtaagagacc
     1921 caaaagtatt taaaagaaag gataaatact ttatgctact tggagcacaa acaagtgata
     1981 aaaaaggagt tataatagta tatgaatcta aaaactctat agattggaat tttaaaggag
     2041 aattaaatat aaaaaatata gatgaagatt ttggttacat gtgggaatgt ccagattata
     2101 ttaatataga tggaaaagat gtattaatat tttcaccaca aggagtagag cctaaaggat
     2161 ttgattatca aaatatatat aatgtagtat atgctattgg taatatggat ttagataatt
     2221 taacatttca aatagatata atgaaagaaa tggaaaaagg atttgatttc tatgctccac
     2281 aaacatttac aaaggaatca caaataatac tatttgcatg ggctggaatg ggagaagttt
     2341 tataccccac agataaaaac aaatgggcac attgtctaac tgttccaaga aaattaaata
     2401 taaaaaataa taagctattg caaatgcctg tagatgaatt aattaaatta agatatgatg
     2461 aaacaattgg gcaaaatact ataaagaata atattaatat tatagaaaat gatgaaaatg
     2521 tatatgagct taacattaat ataaaaaata ttgacagcaa taaatttggg ctggaattat
     2581 tttcatcaca agatgaaggt gtaaaattag aatttaacaa agtaggaaac actgtaactt
     2641 tagacagaag caactttaaa aaagttttcg gtgttgaata tggaacaaat agaaaagaat
     2701 atataaatat tgatgaaaat actaatatca aagtactagc ggatagaagt attttagaaa
     2761 tttttataaa tgatggagaa gtagtattta ctagtagaat atttgctaaa gaaaattcta
     2821 atcaaataag ggtttatagt gataaaatag tagagtataa gtatactaaa tttaaattaa
     2881 agcaaggtat tgaattataa aaatacatta taaggggtag aaaagatgaa aaaagtaatt
     2941 agtataggag aagctttaat agactttata cctaatcaaa atggttgtaa attaaaaaat
     3001 gtatcaggat ttagaagagt agcaggagga gctccagcaa atgttagtgc tgttgtagca
     3061 aagcttggag gaaaatcaag ttttatatct aagttaggaa aagatgcatt tggtgattat
     3121 ataatagatg tattaaatga agtaaatgta aatactgatt atgtattgag aacaagtaaa
     3181 gcaaatacgg gacttgcttt tgtatcatta aaagaagatg gtaatcgtga tttctcattt
     3241 tatagaaatc caagtgcaga tatgctttta gaagctgatg aagttaaaaa agagtggttt
     3301 aatgattgcc atagcctaca tttttgttcg gtagatttaa tagatagtcc aatgaaatta
     3361 gcacataaaa aagcaataga atacgcactt gaaagtaata gtataataag ttttgaccct
     3421 aatataagat tacctttatg ggatagtgag caatcttgta aaaatgcaat atcagaattt
     3481 ttaccttttg ctcacattgt taagatatct gatgaagaat tagaatttat aacaggagaa
     3541 aatgatatag aaaaatcatt acataaactc tttgtgggga atgtggaatt agtattatat
     3601 acaaaaggaa aagatggagt agatgcgtac actaaaaaag taaaaggtat gtgtaaagga
     3661 gttaaagtaa atgcaataga tacaaccgga gcaggagatt cttatatagg ttcattttta
     3721 tacacattgt tgtataaaca aataacattg aatgatataa aagaaatgaa acaagaaacg
     3781 ttaaatgagt atttagaatt ttctaattat tatgcggctc atagtacgac tattaaaggt
     3841 gcgataagtt catatgcaac aaaggaagaa ataactgaat ttataaaaaa tttatagtag
     3901 gaatgagtaa taggttttga aggaggtaac atgtaaagag tttttaagga tgcttactat
     3961 gaaaataatt aatatataaa aagtaataaa gaggctttct caaaatagag ataagcctct
     4021 ttatcattgt tgaaatgata aaaaatattt taattattgt tactttttag aagaatatat
     4081 atcttatttt aaataagcaa attatgtaag atttatttat attatcaata ctttacttgg
     4141 agtattatgt ctttagcagt tatctacttt aattgaatta tctaaaatat aataaatttg
     4201 ttttgcaaat tgacgagaat gttcttcttt ttctttattc ataaaattag gtttacctaa
     4261 atcacgttca acaagcattc ctatatattt taactgtgaa tgcttacagt agcgcttcat
     4321 accattttcc caaatatcag caccctttcc aggtctatat ccacatgttg taataagtgc
     4381 tactggtttt cctgcccata gagcgggacc tttctcatta ccatagtatt tattcattcc
     4441 atacaccaat ctgtcaagta gtgctttcat tggagaagtg cagtaccaag aataaattgg
     4501 agtagcaaaa acaattaaat cacacataag aattttcttg aaaattttct gaacatcatc
     4561 tttacaataa catccaaaat caaataagtt atcctgacat tttttacaag caatgcatgg
     4621 ctctatttta caatcatata accatattac ttcaatattt gaattataat tttctaattc
     4681 ctcaataaaa ggttttaaaa tagcagcagt attacctttt ttcttaggac ttcccataag
     4741 aatgcatact tccatattga tacctccaca gtattttaaa atagttttta gtgcttaata
     4801 tagttttagg atactatgag aatacgttaa caacaataca ctttgtatat ttgtaataaa
     4861 attgaaacaa attgttttac caataaaagt tggaatgtat ctgtaattaa aaatatttta
     4921 ggtatatttt tacatatatg tgtgtagtta actatggtaa tttattgtaa aattatctta
     4981 taggattata attgcttaat ttatgattat atttaaaatc ttggaggtaa aaaatatgta
     5041 ctcaaatgaa gactataaat ttatagaaga aaatgcacca tgttatatat atgatgagac
     5101 taatataatt gaaaaatgta aggaattgaa aaataatatt ttaggttttg attttttata
     5161 ttctataaaa acaaatccat ataacaaaat tgtaaaaagc attgcagagc aaggttttgg
     5221 agcagatgct tcttcaacta atgaagttat gatttcatca aaatgtggag tttctaaaga
     5281 tgatatttat tattcatcac caggcaaaac agagattgat atagaaactg ttttagataa
     5341 atgcataatt attgcagata gtattacaga aatagaactt ataaataaga tatctaaaaa
     5401 gcataatcag aaagttaaaa taggaataag aataaatcct gattttacta tggatgatga
     5461 tagtgctaaa tcaagcaaat ttggtataga tatagaagat atgagcagtg tttttaagat
     5521 gttaagtatg tgtgattatg tatctgtagt tgggattcat atacatatta aatctcaggt
     5581 attggatttt gaaaagttag gacattatta tgaaaaatgt ttttttatag ctttaaattt
     5641 aaataaaagt aagtatatta atattgaatt tataaatttt ggtagtggac ttggagtagc
     5701 ttatgataat cttagagaaa atccacttaa tcttaaagag ctttcaaaat taactcaaaa
     5761 gattgctttt actaattcaa acacactgaa atcaagactt cttattgaat caggacgttt
     5821 catagtttgt aaatcaggta attattatgt taaaattgta gatattaaag tatcaagagg
     5881 gaaaaaatat cttattgtaa aaaatggact taatggattt atgagaccta ttatttctaa
     5941 tttaattgaa aatattactt gtggagtaga attagaaggt caagagccat tatatacatc
     6001 aaatcatgcc ttttctcttt cagtattaaa taatttaaaa gaagaagagt atgtaaatgt
     6061 tgttgggaat ctctgtacat cattagatgt aattgcaaat aatgttaaat tgaaaaaagc
     6121 aaacatagga gatttgattt caattagtaa tgctggaagt tactcctatt ctttatcacc
     6181 actattattt tctagttatg aatttcctaa acaatattat aaaagaaaag atggatatat
     6241 tgagtaagca atagcataaa ttaataaact tagtattatt gaaaaaattt aaaatgcagg
     6301 gggtgtaatt ctatatggaa ttgtggaaaa gaaatttatt tgtatgttgg ataggtatgt
     6361 tttttagttc tatagggatg agccaaatag cacctatact tccattatat attaaacagt
     6421 tgggggtaac tgatgttagt ttaatacaac agtactcagg tataattttt ggatgtactt
     6481 ttgtagtagc agcattcttt tcgccaattt gagggaaagc tgctgataaa tatggaagaa
     6541 aatcaatgct tttacgtgca agtattggga tgggaatagt tatatttacc atggcgtttg
     6601 ttcaaaatgt ttatcagtta ttaggattaa gaatacttca aggagttttt acaggttatg
     6661 caacagcatg tactactctt attgcaacac agacagataa aaatcattca ggatgggctt
     6721 taggtactct tgcaactgca tcaactactg gctcattaat tggtccaaca gtaggaggat
     6781 atatagaatc aatcttaggt cttaaaagta catttattat aacaggagga ttgttgtttg
     6841 tatcatttat catctctatt ctatttgtag tagataattt taaacctaaa gaaataaaag
     6901 aatcaatttc tatacctaaa gaacaattaa atgtattgcc aaataaattt ttaattgcat
     6961 ctatttttgt aaccacattc attactcaac tagctttata ttctatagaa ccaattgtaa
     7021 ccatatatat atcacaactt acaaattttg catcaaatgt agctttaata gctggattga
     7081 cattttctgc atcaggatta gcaaatttat tagcagctca aaaacttgga aaaatgtcag
     7141 ataaaatagg tcctcaaaaa gtattgttaa tttcactttt atgggctgga attatattta
     7201 taccacaagc ttttgtaaaa actgcatggc aactgatgtt gttgagattt ttattagggt
     7261 tgtcagttgc agggcttaat ccttctgtta acagcttgct taaaaaaatt gcaccagaag
     7321 aatatgtggg aaaaatattt ggatataatg catctgccca gtatataggc tgttcatcag
     7381 gagcattttt agggggacaa atatcagcac atttaggtat tagaacagta tttttttcaa
     7441 caagtttatt gttgtttatg aatgcacttt ggatgtataa atttactgga ttgtttatta
     7501 aagataagac taaataatca tactatttac atttctaatt ttatgatgta aaataaatat
     7561 ttcttattta atttatcata gaattataca aaaaataacc ctatggtaaa aattggaaaa
     7621 tattattatc gacaaaatct ttgacttaaa aactatattg cgatagtatt aatttaataa
     7681 aagtttgaga ggggcgtaag gttatgacaa tttcaaaaat accacaagca gaattaaaag
     7741 ttatgaagtt tatttgggaa aaaaatgata cagtaacatc aaaagaaact attgaagcta
     7801 tggaacgaaa atatggttgg aaacaaacaa ctacattgac acttttatca agacttgtta
     7861 aaaaacattt tttaagtgca gaaaaaatag atagatacac tcattataca atagttgttg
     7921 gacacaagga gtatttaagt gttgagacaa aagatttttt aagcaatata cacgacaatt
     7981 ctttacagag cttgatttca gcactacatg atgatgaagt tgttgataaa gatatgttgg
     8041 atttatttga aagtcatttt aaaaatttag aagaagagta attatatcag gatagaactc
     8101 taagaattta attaatctat ggctgaaaac cataaatggt attatttaat cagctactca
     8161 ctattcataa aataatatta aataggttct aattagtaaa atgcctaaaa taattggaat
     8221 agggagtatc aagttaaatg ataccactta tttaagtaag aaatgatttt taaaatttaa
     8281 aataatttta taacttgtca atattc
//
LOCUS       DAEPXF010000166         8259 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain HB27
            SAMN13376389-rid12479423.denovo.173, whole genome shotgun sequence.
ACCESSION   DAEPXF010000166 DAEPXF010000000
VERSION     DAEPXF010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13376389
            Sequence Read Archive: SRR10518432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8259)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8259)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001069624.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001069624.1
            Genome Coverage       :: 315x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/15/2021 01:54:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,165
            CDSs (total)                      :: 4,076
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 89
            rRNAs                             :: 10, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 10 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 19 of 69
            Pseudo Genes (incomplete)         :: 44 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 10 of 69
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8259
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13376389-rid12479423.denovo.173"
                     /strain="HB27"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="China: Hebei"
                     /collection_date="2017"
     gene            <1..699
                     /locus_tag="KRL67_003108"
     CDS             <1..699
                     /locus_tag="KRL67_003108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBH1523971.1"
                     /translation="TIFENLSIDIEDGEFVSIIGSNGTGKSTLLNIISGLIKESSGQV
                     LLNKHNLSNLAEHRRTQIVSRVFQDPSLGTCPSMTVRENLSLALNKGKLLNLRKCLRY
                     KRDFLENLLEGVSLDLKKYLDVQVKFLSGGQRQSLSLIMSCLTNPSVLLLDEHTAALD
                     PKTSNEVIELTNKIVREKNITTLMVTHNLKHALQYGDRLIMLHKGEVVLDVKGKEKEQ
                     LTVEEILEKFEYAV"
     gene            830..1852
                     /locus_tag="KRL67_003109"
     CDS             830..1852
                     /locus_tag="KRL67_003109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBH1523972.1"
                     /translation="MINKKRLASLILAGALSVSMLTGCSQGGDSGNSKQESNSKSKEV
                     KKIGITQLVEHPALDATKAGFVKALEKNGFKDGENIDIDFQNAQNDMPTTQSIASKFA
                     SDKKDLIFAISTPSAQAAFNATKDIPILMTAVSDPVAAGLVKTLEKPGTNVSGTSDFV
                     SVDKGLELLKIFAPKAKTIGVMYNTSEVNSKVQVDALKEYASKNGFKVVEKGITTSNE
                     VNQGISSLVGKIDVLYVPTDNLVASSMPIVSKIATENKIPVIAAESGPVEKGALACQG
                     INYEKLGYKTGEMAVKILNGESVSDMPVATSDDTDIIVNEDILKALGMEKPSNDNISY
                     VKTKQE"
     gene            complement(2016..2573)
                     /locus_tag="KRL67_003110"
     CDS             complement(2016..2573)
                     /locus_tag="KRL67_003110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HBH1523973.1"
                     /translation="MTEKEKMLSGKGYYANDELLVKEREYCKKLTRLFNNTLEDEYEK
                     REGILRQLFGSVGKQINVEQNIRCDYGYNIHVGENFFANYDCIFLDVCKIEIGDNVML
                     APNVQIYTAYHPIDAQLRNSGIEYGSPVKIGDNVWIGGGVIITPGITIGDNVVIGAGS
                     VVTKDIPPNTVAVGNPCRVIKKIEE"
     gene            complement(2579..3655)
                     /locus_tag="KRL67_003111"
     CDS             complement(2579..3655)
                     /locus_tag="KRL67_003111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/molybdate ABC transporter ATP-binding
                     protein"
                     /protein_id="HBH1523974.1"
                     /translation="MSLYVDIEKDLSSFKLKVEIKQEKGTLGFLGESGSGKSMTLKCI
                     AGLEKPTRGKIVLNDRVLFDSEKKINLSTQDRKVGFLFQNYALFPHMTVSQNIELGLL
                     KLSKSEKKEIVARYLDILKLNGFEGRYPWQLSGGQQQRVALARALATSPDILLLDEPF
                     SALDHHLRSNMEKELMNMLKDYKGDILFVTHDIEEAYRVCDDIIVYNKGEGLPKRPKK
                     ELFESPKYLIEAKITGCKNISKLNRLDKNTIYATDWGCELTLNREIGDNIEYVGIREH
                     HIKVLDSNEGLNEKLCFELINIVENPFTYTIYVRKTDLSNECIPIQIELEKSKMRFKK
                     GDSIYLDFPHEYLFCFRYNYNKKE"
     gene            complement(3656..4339)
                     /gene="modB"
                     /locus_tag="KRL67_003112"
     CDS             complement(3656..4339)
                     /gene="modB"
                     /locus_tag="KRL67_003112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter permease subunit"
                     /protein_id="HBH1523975.1"
                     /translation="MGTDWSPLWISLKTSILSTVITFVIGIFVSYIMANYRGKWKGLI
                     DGLFTLPLILPPTVVGFFLLLLCGKNGFIGKFLLNFDRTLIFSWTATVISAVVVSFPM
                     MYRTSRSAFEQIDNNMLSAARTLGLNEWKVFFKIAVPLAWPGIIGGLVLSFARALGEF
                     GATLMIAGNIPGKTQTMPIAIFFAVEGGDMNKAMMWVIIIVAISIAMILLLNYWSEFQ
                     QKIIGKRCG"
     gene            complement(4488..5300)
                     /gene="modA"
                     /locus_tag="KRL67_003113"
     CDS             complement(4488..5300)
                     /gene="modA"
                     /locus_tag="KRL67_003113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HBH1523976.1"
                     /translation="MKKILGILGLVACLTLGTVGCNSNEDKKDDGKQEKTNKSSDSVE
                     LNISAAASLKEAMAKIEEEYKKVDSNVKLTVNYGASGSLQQQIEQGAPCDLFISAGQK
                     QMKALDEEKLLISDTMKDLVKNDLVLISSADSSVSGMKDLTTDKVKKIAVGEAESVPA
                     GKYADEVLTNLNLKDKLKDKLVFAKDVKEVLAWVQSGNADVGFVYFSDTVNNDKIKVV
                     EKTDEKTHSPITYPVSVIKASKNVDATKKFEEFLLSESGQKIFEEFGYKKVE"
     gene            5493..6422
                     /locus_tag="KRL67_003114"
     CDS             5493..6422
                     /locus_tag="KRL67_003114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBH1523977.1"
                     /translation="MDSLSSLTPVEVAELLKITKNTVYELIKRGELPSYKVGKKIRVD
                     TKDVEEYINSQKTVKVKDTHFNNKNIDINNNFKIIISGQDVILDILARSVEKKLDGIS
                     TFRSYIGSYNALYELYNDRVSIASSHLWDFETDEYNSTFVKKLLPGIPCVLINLAYRM
                     QGFYVAKGNPKEITTWEDLTKPSITIVNREKGSGTRVLLDGKLRLLNFNGKHIKGYDN
                     EELSHLGVASTVSRGIADVGLGNEKAALQVNNIDFIPLHKERYDLVIKKEDLQNPVYQ
                     TIINIINSPEFKAELQGLGGYDLTDTGKTIAKV"
     gene            complement(6595..7044)
                     /locus_tag="KRL67_003115"
     CDS             complement(6595..7044)
                     /locus_tag="KRL67_003115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH1523978.1"
                     /translation="MKNKNNKDKNMLNIKVLRVLGLLLILFDYTGDTLNFYNFFAKPL
                     VSGNLYKTTGTDCIVFVLFIVECVYLVGRYILKVKINWSEIIENEKDNFINNLLVEKI
                     QEESNIVYNLLNSYVLPHIYKKGRVLMFKTYHMLIYIKEKYYYVNIY"
     gene            complement(7251..8231)
                     /locus_tag="KRL67_003116"
     CDS             complement(7251..8231)
                     /locus_tag="KRL67_003116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBH1523979.1"
                     /translation="MKNSFKNCISLRNKFIIISIVFLLILLIVSYIFGKLVYNSSVGL
                     EQLIKNEDIVKIFSERKDKPLEKLKNYKTSELMIQSSNNYKLESLFITSNIKTRDTMI
                     LVHGIGSSYYEMLKVAYRYLDKGYNVLVYNQRNTGNSGGDNYTFGLYERYDLDSLAKF
                     VKNKFPEGRLGVHGFSMGAGTAAMHTELNSKDDKVDFYILDSPYSEMKDAIRMGVLEK
                     RIPYILINYVVICGDLYNKFKSGFWYSDVKPYESVEKSNVPILFIHGTKDTVCNYQNS
                     KKMYDLVKHDKKELWLIEGIGHVDGFEHDSTVYFNKIFKFIDSNVLSDKS"
BASE COUNT         2860 a         1344 c          998 g         3057 t
ORIGIN      
        1 actatatttg aaaatttatc aatcgatata gaagatggtg aatttgtaag tatcataggt
       61 agtaatggaa ctggtaaatc tactttatta aacataatat ctggacttat aaaagaatct
      121 tctggtcaag tattattaaa taaacataac ttgtctaatt tagctgaaca cagaagaact
      181 caaatagtaa gtagagtttt tcaagaccca agtcttggaa cttgtccttc tatgactgtt
      241 agggaaaact tgtctttagc tcttaataaa ggaaaactat taaatttaag aaaatgtctt
      301 cgttacaaaa gagatttttt agaaaattta ttggaaggtg tatctcttga tttaaaaaaa
      361 taccttgatg ttcaagtaaa atttttatca ggaggtcaaa gacaatcatt atcactaatt
      421 atgtcgtgct taacaaatcc atcagtttta ttacttgatg aacatacagc agcacttgac
      481 ccaaaaacat caaatgaagt aattgaattg actaataaga ttgtaaggga aaaaaatata
      541 actacactta tggttactca caacttaaaa cacgctcttc agtatggtga tagattgata
      601 atgcttcata agggtgaagt tgtattagat gtaaaaggta aagaaaaaga acaattaaca
      661 gttgaagaaa tcttagaaaa atttgaatat gcagtttaaa ataatatatc tattgtatta
      721 aactcaactt taaaataaag ttaaagctat aataattatg gttactttgt aataattcat
      781 atgaatatat gaacatatat aataataaat ttttgagggg gatataaaaa tgattaataa
      841 aaaaagattg gcaagtttga ttttagctgg tgctcttagt gtatctatgc ttacagggtg
      901 ttcacaagga ggagactctg gtaattcaaa acaagaaagt aattctaaaa gtaaagaagt
      961 taaaaaaatt ggtataactc aactggttga acatccagca ctagatgcca ctaaggcagg
     1021 ttttgtaaaa gcattagaaa agaacggatt taaagatgga gaaaatattg atatagactt
     1081 ccaaaatgct caaaatgata tgcctactac acaaagtatt gcaagtaaat ttgcatctga
     1141 taagaaagat ttaatatttg ctatatctac tccgtcagct caggcagcat ttaatgctac
     1201 taaagatata cctatactta tgactgctgt gagtgaccct gttgctgctg gattagttaa
     1261 aactcttgaa aagccaggca caaatgtatc tggaacatca gattttgttt ctgtagacaa
     1321 aggtttagaa cttcttaaaa tatttgctcc taaggcaaaa actataggag ttatgtacaa
     1381 tactagtgaa gtaaattcca aagttcaagt tgatgcttta aaagaatatg catctaaaaa
     1441 tggctttaaa gttgttgaaa aaggtataac tacttcaaat gaagttaacc aaggtatttc
     1501 tagtttagtt ggcaaaatag acgtattata tgttcctaca gataacttag tggcttcttc
     1561 tatgcctata gtctctaaaa tagctactga gaataaaata cctgttatag cagctgaatc
     1621 tggtccagtt gaaaaaggtg ctcttgcttg tcaaggtatc aactacgaaa aactaggtta
     1681 caaaacaggt gaaatggcag ttaaaatttt aaatggtgaa tcagtatctg atatgcctgt
     1741 agctacatca gatgacacag atatcattgt taatgaagat atattaaaag ctttaggtat
     1801 ggaaaaacct tctaatgata atatttctta tgtaaagact aaacaagaat agaaagatat
     1861 tttctctata cgattaatat gcctgtttct ataaattaat ttatagaaac aggcatatat
     1921 ttttatttta atgtattata tatttaaaga ttatatgtaa atactcttaa tttgttttta
     1981 taaattttac ttgtataaac tttatacaat catatttatt cttctatctt ctttattact
     2041 ctacatggat ttccaacagc tacagtattt ggtggtatat cttttgtgac tacacttcct
     2101 gcacctatta ctacattatc acctatagta attcctggtg ttatgattac tcctccacct
     2161 atccatacat tatcacctat ttttacagga gagccatact ctatacctga atttctaagt
     2221 tgtgcatcaa ttggatgata ggctgtatag atttgaacgt ttggagctaa cattacattg
     2281 tcacctattt caattttaca tacatctaaa aaaatacaat cataatttgc aaagaaattt
     2341 tcgcctacat gtatgttata tccataatca cacctaatgt tttgttctac atttatttgc
     2401 ttgccaactg aaccaaatag ttgtcttaaa ataccttctc ttttttcata ttcatcttct
     2461 aaagtattat taaacaatct agttaatttt ttacaatact ctctttcttt tactagcaat
     2521 tcatcatttg catagtatcc tttcccggac aacatttttt ctttttcagt catatagttt
     2581 actccttttt gttataattg tatctaaaac aaaataaata ttcgtgtgga aaatctaaat
     2641 atatgctgtc acccttctta aatctcattt tacttttttc caattctatt tgaataggta
     2701 tacattcgtt agataaatca gtttttctaa catatatggt atatgtaaaa ggattttcta
     2761 caatgttaat caattcgaaa cataactttt catttaaacc ttcattagaa tccaagacct
     2821 taatatgatg ctccctgatt cccacatatt ctatattatc acctatttct ctgtttaatg
     2881 tcaactcgca tccccaatct gtagcataaa tagtattttt atccagtcta tttaatttgg
     2941 atatattttt acaccctgtt atcttagctt ctataaggta ctttggagat tcaaagagtt
     3001 cttttttagg tctctttgga aggccttctc ccttattata cactataata tcatcacaaa
     3061 ctcggtaggc ttcttcaata tcatgagtaa caaatagtat atctcccttg taatctttta
     3121 acatattcat aagttctttt tccatattgc ttcttaaatg atggtccagt gctgagaaag
     3181 gttcatctaa aagtaatata tctggagatg ttgccaatgc tctagctagt gctacccttt
     3241 gttgttgacc accagataat tgccaagggt atctaccctc aaacccattt aatttaagta
     3301 tatctaaata ccttgctact atttcctttt tttcactctt gcttagtttt aaaagcccaa
     3361 gttctatatt ttgacttaca gtcatatgtg gaaacaaagc atagttttga aataaaaaac
     3421 ctacctttct atcttgtgtt gataaattta tcttcttttc agaatcaaat agaactctat
     3481 catttaatac aattttacct cttgttggtt tctcaagacc tgctatacat tttaatgtca
     3541 tactttttcc agaaccagat tctcccaaaa aacccagagt acctttttct tgtttaattt
     3601 ctacctttag tttaaaagag gataaatctt tttcaatatc tacgtataag ctcaactatc
     3661 cacacctctt tcctataatt ttctgctgaa attccgacca ataatttagt aataaaatca
     3721 ttgctataga tattgctaca ataattataa cccacatcat agctttattc atatctccgc
     3781 cttcaacagc aaaaaatata gctattggca tagtttgagt ttttccaggt atatttccag
     3841 ctatcataag tgtagcacca aattcgccaa gagctctggc aaacgacagc accaatccac
     3901 caattattcc aggccatgct aatggaactg ctatcttaaa aaatactttc cattcattaa
     3961 gacctaatgt cctagcagct gacaacatat tattgtcaat ctgttcaaaa gctgaccttg
     4021 aagttctata catcatagga aaagacacca ctactgctga aattacagtt gctgtccaac
     4081 taaagattaa agtcctatca aaatttaata aaaactttcc tataaatcca ttcttcccac
     4141 atagtaataa taaaaagaaa cctacaactg ttggtggcaa aattagtgga agtgtaaata
     4201 gtccatctat aagacccttc cacttacctc tatagttagc cataatatat gacacaaata
     4261 ttcctattac aaaagttatt actgttgaca aaatagatgt cttaagtgat atccataagg
     4321 gtgaccaatc tgttcccatc tttccctcct taattttaaa tggaaatagt gaaactaata
     4381 actctattaa tttcactatt tccattttat tataatacac aattattttc aattttcatt
     4441 tatttatata taatttatac tcaattatac ataatttatt tataagttta ttctactttt
     4501 ttatatccaa attcttcaaa aatcttttgt cctgactcac ttaataaaaa ttcttcaaat
     4561 ttcttagtag catctacgtt tttactagct ttaattactg aaactggata tgtaataggt
     4621 gaatgagttt tttcgtctgt tttttcaact acttttatct tatcattatt gacagtatca
     4681 ctgaagtaaa cgaatccaac atcagcattt cctgattgca cccatgctaa aacttctttt
     4741 acatcttttg caaatactag cttatctttt aacttatctt ttaaatttaa attagttaac
     4801 acctcatctg catatttacc agctggaaca ctttcagctt caccaacagc aattttcttc
     4861 actttatctg tagtcaaatc tttcatacct gatacactac tgtcagcaga agaaataaga
     4921 actaaatcat tttttactaa atctttcata gtatcagaaa ttaataactt ctcttcatcc
     4981 aaagctttca tttgtttttg acctgctgaa ataaataaat cacaaggtgc tccttgttct
     5041 atttgttgtt gtaatgagcc tgatgctcca tagttaactg taagttttac atttgaatca
     5101 acttttttat attcttcttc aatctttgcc attgcttctt ttaaacttgc agcagccgat
     5161 atatttaact ctactgaatc actactttta tttgtttttt cttgttttcc atcatctttt
     5221 ttgtcttcat tactattgca cccaactgtc cccaaagtta aacatgcaac taatcctaat
     5281 attcccaata tttttttcat aacaatctcc ctttttgtaa atttgataca tattttaaca
     5341 tgttttatta tgttttatca tgttttgtta tatatagttt aattatatat acatgataat
     5401 aaattttcaa gtaatttatt tacactactt ttaaatattt tgctacattt tccttttttt
     5461 aatgttaaaa taaccttata tggaggtgga ttatggactc attatcatct ttgacacctg
     5521 tcgaggtagc tgaattatta aagataacaa aaaatactgt atatgaatta atcaaaagag
     5581 gtgaacttcc ttcttataaa gtagggaaaa aaataagagt tgatactaaa gatgttgaag
     5641 aatatatcaa cagccaaaaa actgttaaag taaaggatac acatttcaac aataaaaata
     5701 tagatataaa taataatttc aaaattatta tctctggtca agatgtaata ttagacattt
     5761 tagctaggtc tgttgaaaaa aaattggatg gaataagtac atttcgctct tatataggta
     5821 gttataatgc tttgtatgag ttatataatg acagagtttc tatagcatca tcccatctat
     5881 gggatttcga aacggatgaa tataatagca cttttgttaa aaagctattg ccaggaatac
     5941 catgtgtact cataaatcta gcttatagaa tgcaaggttt ctatgttgct aaaggaaatc
     6001 ctaaagaaat aacaacctgg gaggatttaa caaaaccaag cataactatt gtaaatagag
     6061 aaaaaggttc tggaactagg gttttactag atggtaaact tagacttcta aactttaatg
     6121 gtaagcatat aaaaggttat gataatgaag agttatcaca tcttggagtt gcaagtactg
     6181 tttctcgtgg aatagcagat gttggtttgg gaaatgaaaa agctgcttta caagtaaata
     6241 atatcgactt tattcctctt cacaaagagc gctatgattt agttattaaa aaagaagact
     6301 tacaaaatcc agtttaccaa actataatta acataataaa ctctcctgag tttaaagctg
     6361 aacttcaagg ccttggcgga tatgatttaa ctgatactgg aaaaactata gcaaaagttt
     6421 aagattaaaa tcgcaatcta ttttataaga tttataattt ttcacaaaat ttataactta
     6481 aacacgaaat aaattgcgat tttagtgtct caaaaaattt atgtctttca ttatgtcttt
     6541 tattttatca tctaatacaa aatttaatat aattaattgt ataatcatat tatattaata
     6601 tatatttaca taatagtatt tttctttaat atagattaac atatgatatg ttttaaacat
     6661 cagtaccctt cctttcttat atatatgtgg caacacatat gagtttaata aattatatac
     6721 aatattgctt tcttcttgta ttttttcaac caacaagtta tttataaaat tatctttctc
     6781 attttcaata atctcactcc aatttatctt aacttttaat atatatcttc ccactaggta
     6841 tacacactct acaataaata atacaaatac aatgcagtca gtacctgttg tcttatataa
     6901 attacccgat actaagggct ttgcgaagaa attataaaaa ttcaaagtat cacctgtata
     6961 atcaaaaagt attaatagta gtcctagaac tctcaatact ttaatattca acatgttctt
     7021 atctttatta tttttgtttt tcatatatac tacctctctt ttctttagat tttattgtaa
     7081 tccaatacta actcttttat ttgaggtata aatgaattta attggtagtt attttcattt
     7141 ttactagaaa ttaattcaca ttactaaaca aaacttatat ttaaacataa aaatagccta
     7201 tctatttttt aatatataaa tacaaaaata aataagctac taatattcaa ctaactttta
     7261 tcacttaaaa cattactatc tataaattta aatattttat tgaagtatac tgtactatca
     7321 tgttcaaatc catcaacatg accaatacct tctataagcc acagctcttt tttatcatgt
     7381 ttaactaaat catacatttt cttgctattt tgataattac aaacagtatc ttttgttcca
     7441 tgtataaaca gtatgggtac attagatttc tcaactgact catatggttt tacatcactg
     7501 taccaaaatc cagatttaaa cttattatat aaatctccac aaatcactac ataattgatt
     7561 aatatatacg gtattcgttt ttctagtact cccattctaa ttgcatcttt catttcagaa
     7621 taaggagaat ccagtatata aaaatcgacc ttatcatctt ttgaatttag ctctgtatgc
     7681 atagcagctg ttcctgcccc catggaaaat ccatgcactc ctaatcttcc ttcaggaaat
     7741 ttatttttta caaatttagc tagactatct aaatcatacc tttcataaag accaaatgtg
     7801 taattatctc caccactatt accagtattt ctttgattat aaactaatac attataacct
     7861 ttatctaaat atctatatgc tacctttaac atttcatagt aactacttcc aattccatga
     7921 acaagtatca tagtatctct tgtctttata tttgaagtta taaaaagact ttcgagcttg
     7981 taattattag aactttgtat cattaattca gatgtcttgt agttttttaa tttctccagt
     8041 ggtttatctt ttctctcaga aaatattttt actatatctt cgttttttat taactgctct
     8101 aaaccaaccg aactattata cactaatttg ccaaatatat aagatacaat taataggatt
     8161 aatagaaaca caattgatat aattataaat ttatttctta agctaataca atttttaaaa
     8221 ctatttttca tatttattta ccatacaatt ttcctatat
//
LOCUS       DAEPXF010000167         8090 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain HB27
            SAMN13376389-rid12479423.denovo.174, whole genome shotgun sequence.
ACCESSION   DAEPXF010000167 DAEPXF010000000
VERSION     DAEPXF010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13376389
            Sequence Read Archive: SRR10518432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8090)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8090)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001069624.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001069624.1
            Genome Coverage       :: 315x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/15/2021 01:54:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,165
            CDSs (total)                      :: 4,076
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 89
            rRNAs                             :: 10, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 10 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 19 of 69
            Pseudo Genes (incomplete)         :: 44 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 10 of 69
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8090
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13376389-rid12479423.denovo.174"
                     /strain="HB27"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="China: Hebei"
                     /collection_date="2017"
     gene            148..1524
                     /locus_tag="KRL67_003117"
     CDS             148..1524
                     /locus_tag="KRL67_003117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk family potassium uptake protein"
                     /protein_id="HBH1523980.1"
                     /translation="MKTIVRRLILYADGMRPTQIMVSGFAAIIVIGALLLTLPIASQS
                     GESIGLLNALFTATSAVCVTGLVMVDTATYWSLFGQIVIITLIQIGGLGFMTVATMFS
                     LMARKKIQLRERLLIQESLNQADLSGLVRLTRFVLIITITIEGIGALVLSTVFIPQFG
                     LSKGIWYSVFHAISAFCNAGFDLMGSVSGPFTSLNSYVNNFTVSMTVCALIVLGGLGF
                     PVVLDIVRKRRFSKLNVHSKVVLFSTATLIFVGALFIFLIEFNQKATMADLPLKGKVL
                     SAIFQSVTARTAGFNTLDLATLRESSVFVMIILMFIGASPASTGGGIKTTTLAVLIIT
                     VRSFLSGKSDIEAFERRLAPATIKKSLGIFVISISAVIFGTLIISITQPNFTLVQSAF
                     EVTSALATVGSSLAGTPNLNALGKIIIIIFMFMGRVGSLTLFMAILSGGRRKSQPIRY
                     AEGKIMVG"
     gene            1824..2495
                     /locus_tag="KRL67_003118"
     CDS             1824..2495
                     /locus_tag="KRL67_003118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HBH1523981.1"
                     /translation="MKQYIVIGCGRFGSSVASTMHLLGHQVMAIDKNEDSVQSISDKV
                     THSLIVDVTDEQALRSLGLGNFDVAVVAIGSDIRASIMATLIAKEMGVELIICKAKDE
                     LQAKVLYKIGADRVVFPERDMGVRVAHNLVSDNILDHIELDPEYSIVEIVTPNSWVGK
                     TLIELELRARYEITVLAIKTGKNINVTPSPDEELTAGSILVIIGQNTSITAITSGNKG
                     IIRRR"
     gene            2684..3481
                     /locus_tag="KRL67_003119"
     CDS             2684..3481
                     /locus_tag="KRL67_003119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HBH1523982.1"
                     /translation="MITNINSKDNEKLKYTRALLKSKNRNKESKFIIEGYRIVMLALE
                     CMANLDYVFINEEFENKKEHVKLLEDLDKKNTKIYKTTNKNFKELVDTENTQGIIGVV
                     SFKKKKLSESINKKDKFVLILDRIQDPGNMGTIIRTADSAGVDAIIALKGCVDIYNPK
                     VIRSTMGSIFDMNVIDASQDETVDMLKSLDFNIVSSYLNTDNFYDKIDYGSKVALVIG
                     NEANGINEELVSKSNILVKIPIYGKAESLNAAISSAILMYEIKKYLI"
     misc_binding    3657..3890
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            3940..4959
                     /gene="pheS"
                     /locus_tag="KRL67_003120"
     CDS             3940..4959
                     /gene="pheS"
                     /locus_tag="KRL67_003120"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000388219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HBH1523983.1"
                     /translation="MQEKLLALREAALAEIKEAQSIESVESLRVKYLGKKGEITTILK
                     EMGKLSAEERPVVGKVANEVRENIELSINSKKEEINAIEKERKLKEEVIDVTQPGKVL
                     KVGKKHPITQIIDEVTDIFIGMGFSIAEGPEVETVENNFDALNAPKDHPSRDMSDTFY
                     INDGVLLRTQTSPVQVRTMRSQELPIKVIAPGRCFRSDSPDATHSPMFHQIEGLVVGK
                     DVTMAEFKGTMDIFVEKLFGSDIKTKFRPHNFPFTEPSAEVDVTCFKCGGKGCPMCKY
                     EGWIEILGSGMVHPNVLRNCGIDPEVYSGFAFGVGVERLAMLKYEIDDIRLLFENDMR
                     FLNQF"
     gene            4976..7369
                     /locus_tag="KRL67_003121"
     CDS             4976..7369
                     /locus_tag="KRL67_003121"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HBH1523984.1"
                     /translation="MLVSLKWLRDYVDIDMDVKEFADKMTMTGTKVETIDYYGEEIEN
                     ILVGKILEIKQHPNADKLVVTKVDIGDKVVQIVTGATNISEGDYIPVAINGSKLPGGV
                     EIKQTDFRGELSDGMMCSAAELGIDEHYIEEYKRGGIYILDHEDSYELGKDIKDVLGL
                     KDALIDFELTSNRPDCKCMMGIAREAAATIGTKVKYPEIEVKESDEEIDFKVEIDNPD
                     LCRRYVARMVTDVKIEPSPYWMQRRLTEAGVRPISNIVDITNFVMLELGQPLHAFDIN
                     QVETGRIVVRNAKDGEKLVTLDDVERTLDKDMLVITNGEKSLGLAGVMGGANSEITSN
                     TKTVLFESANFKPENIRMTAKKVGIRSEASSRNEKDLDPNLAEIAANRAVQLVEMLGA
                     GKVLKGVVDVYPNKPEPKKLVVNPQRINHLLGVDVPMEQFVGILESLEFKCNLVADDK
                     LEIDVPSFRTDMEQEADVWEEIARIYGFENIPSVQLEGNTTAGIKTSKQKFMDALKDN
                     STAVGLNEILTYSFVSPKGVDKIRVPEGNAKRNFVKLLNPLGEETSVMRTTLIPNMLD
                     VLSTNVSHKIEEVSAFECGHIFIPQDSELPKEENRMCVGMYGKDVDFFALKGTIETIL
                     VNVGFKGYEIEPQDNNTTFHPGRCAKIVYNNKYVGTLGELHPDVIENYNLGQRVYVAE
                     IDIDFVFDNSDRTKNYVPLPKYPSTSRDIALIVKDDVFVKQIEDIIKENGQGLVESYK
                     LFDVYKGSQIEAGYKSIAYSITYRSKDKTLTDEDVAKVHDKILSELSEKLNANLRSN"
     gene            7394..7984
                     /gene="zapA"
                     /locus_tag="KRL67_003122"
     CDS             7394..7984
                     /gene="zapA"
                     /locus_tag="KRL67_003122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HBH1523985.1"
                     /translation="MNKVMVKIHGAEYPMVGDKSEKFMISIADFVDKEMDKITRQNPK
                     LSLSVAAILTALNISDLLFECSDENEKLIKANEELSKKVGASNEELQLEIKSLKLTIA
                     EKEAENREAETKMKELIEIIENKKQEIFELSNTTEGSRAELDAYKNKIEELSTQLEEA
                     NERATIAENLASEFQNKAYDLQLKCTGLNNDAKNVE"
BASE COUNT         3141 a          925 c         1586 g         2438 t
ORIGIN      
        1 tctaaacaga aattataaaa attactatca ttgtctttat acaatattta tactataata
       61 gaataataat ttagaggaaa gctctaaaaa taaacaaatt aagggtctta acagtagtga
      121 ttttatgtta aggaggtata aagaaagttg aaaacaatag ttagaagact tattctctac
      181 gcagatggaa tgcgtcctac tcaaataatg gtatctgggt ttgctgcgat tattgtaatc
      241 ggagcattgt tgttaacact cccgatagcg tcgcagagtg gagagagtat aggcttatta
      301 aatgctctat ttacggctac ttcagcggtt tgtgttactg gtctagttat ggtagataca
      361 gcaacttatt ggagtttatt tggacagata gttataataa cattgataca aattggtggt
      421 cttggattta tgaccgtagc cacaatgttt tcacttatgg cacgaaaaaa aatacaacta
      481 agagaaagat tattaataca agagtcattg aatcaagctg atttatcggg acttgtaaga
      541 cttacaagat ttgtattaat aataaccata acaatagagg gaataggtgc actagtttta
      601 tcaactgtct ttattccaca atttggtttg tctaaaggga tatggtatag tgtatttcac
      661 gcaatatcag cgttttgtaa tgccggattt gacttgatgg gcagtgtaag tggcccattt
      721 acatcgttaa attcttatgt aaacaacttt acagtatcaa tgactgtttg cgcacttata
      781 gtgcttggag ggctaggatt ccctgttgta cttgatattg taagaaaaag aagattttca
      841 aaattaaatg tgcattctaa ggttgtattg ttttcaactg ctacacttat ttttgtgggt
      901 gcattattta tttttcttat agaatttaat caaaaagcta ctatggcgga cttgccttta
      961 aagggaaaag tactatcagc aatatttcaa tcagtaacag caagaactgc tggttttaat
     1021 acgcttgatt tagctacttt acgagaaagt agtgtatttg taatgataat tttgatgttt
     1081 attggagcat cacctgcatc aactggtgga ggtataaaaa caacaacact agctgtttta
     1141 atcattacag ttagaagttt tctatcagga aagtcagata tagaagcttt tgaaagaaga
     1201 ttagctccag caactataaa aaaatctctt ggtatatttg taatcagtat atcagcagtt
     1261 atttttggga cattgattat ttcaataact cagcctaatt ttacattagt tcaatcagct
     1321 tttgaggtta catcagccct tgctacagtt ggttcaagtt tagctggaac tccaaactta
     1381 aatgctttag gtaagattat aataataatc tttatgttta tgggtagagt tggttctctt
     1441 actttattta tggctatact ttctggtggt agaagaaaga gtcaaccaat cagatatgca
     1501 gaaggtaaaa ttatggttgg ttaagttacc aaggagtagt ataaatttat aatgatttag
     1561 agtagcactg taaatctgtt gataaaataa atgaacctag atgtaattat atgaagaaat
     1621 aaaatcgact aacgaacaaa attaattaat aaaataaaaa acagtgaaat aaaaaacagt
     1681 gaaataaaat acaaataaaa aaggtaaatc aaaattaaat taatctatgt atataaaatt
     1741 atatattgtg tataagtttt gtatgtgcaa acattaattt aagagatact atataaattg
     1801 tgtattaggg aggaagtttc gagatgaagc agtatatagt cattgggtgt gggagatttg
     1861 gaagttcagt tgcgtctact atgcatcttt tagggcatca agtaatggca atagacaaaa
     1921 atgaagattc agttcaaagt atatctgaca aggtaaccca ttcacttata gtggatgtta
     1981 ctgatgagca agcgttaagg tcattaggtt taggtaactt tgatgtagca gtagttgcaa
     2041 taggttctga tataagggca tctataatgg cgactcttat agccaaagaa atgggtgtag
     2101 agttgataat atgtaaggca aaggatgaat tacaagctaa agtgctttat aaaattggcg
     2161 cagatagagt tgtatttcca gaaagagata tgggagtaag agttgcacac aatttagttt
     2221 cggataatat attagaccat attgaacttg acccagagta ttcaattgtt gaaatcgtaa
     2281 ctccaaatag ttgggttggc aagacactta tagagcttga attaagagct agatatgaga
     2341 taactgtact tgctataaaa acaggtaaaa atataaatgt tacaccttct ccagatgagg
     2401 aacttacagc tggaagtatc ctagttataa tcggtcaaaa tactagtata acagcgataa
     2461 catctggaaa taaggggata attagaagaa gataatttac tatttaatat atatttaatt
     2521 gcaatgaaag tagatagtat catataatta tgaatagtta tatgatacta tttttttaat
     2581 cgaaggtagt atttatttgt aagattagat aaagatgagt taaattaaaa gtgaggaggc
     2641 tatgctaata taaaaattta taagttatta gcatttagat aaaatgatta caaatataaa
     2701 cagtaaggat aatgaaaagt taaagtatac aagagcacta ttaaaatcaa agaataggaa
     2761 taaagagtca aagtttataa tagaaggata cagaatagta atgcttgcac ttgaatgtat
     2821 ggcaaacctt gattatgtat ttatcaatga ggaatttgaa aataagaaag aacatgtaaa
     2881 actattagaa gatttggata aaaaaaacac aaagatatac aagactacta ataaaaactt
     2941 taaagaatta gtggatacag aaaatactca aggaataata ggtgtagttt catttaagaa
     3001 aaaaaaatta agtgaaagta taaataaaaa agataaattt gtattgattt tagatagaat
     3061 acaagaccca ggaaatatgg ggactataat aaggactgct gattctgctg gggtagatgc
     3121 cataatagca ctaaagggat gtgtcgatat atacaaccca aaagtaatta ggtctactat
     3181 gggttctatt tttgatatga atgtaattga tgcttcacaa gatgaaactg tggacatgct
     3241 taaatcattg gattttaata tagtttcaag ttacttaaat acagataatt tttatgacaa
     3301 aatagattat ggttcaaaag tagcattggt gataggaaac gaagcaaatg gaataaatga
     3361 agaacttgta tcaaagtcta atattttggt taagatacct atatatggta aagctgagtc
     3421 gttaaatgct gcgataagct ctgctatact gatgtatgaa ataaaaaaat acttaattta
     3481 atgtattgta ataaatataa tgtcatgtta taatcttgaa ttaaatagta gagtatcaaa
     3541 aatagtcaat atatattaaa aaaataatta aattaattat gtattggata tatattaaaa
     3601 atagtcacat aatttgaatg gaaatgatat aatactaaaa taaacaatat aatattgtaa
     3661 atgcaatgaa agaggaaagt attttgacta aacttagtaa agagataaac acctaggctg
     3721 ggagtgtttt ctaagaggtc atgagaagtt ccctctggag taacagagct gaaattttac
     3781 agtaggcttt gacgtcaaaa acgcgttaag tttgttagag gtggtttgat gattttaaat
     3841 tgttaaacta ctagggtggt accgcgaaac tatagccttt cgtccctaga cagggattga
     3901 ggggcttttt ttatacaaaa aaacgaaagg gtgatatgtg tgcaagaaaa attacttgct
     3961 ttacgtgaag cagctttggc tgaaataaaa gaagcacaaa gcatagaaag tgtagaaagt
     4021 ttaagagtta agtacttagg aaaaaaaggt gaaataacta ccatacttaa agaaatgggt
     4081 aaattatctg ctgaagaaag accagtagtt ggtaaggttg caaatgaggt aagagaaaac
     4141 attgaactta gcataaattc taaaaaagaa gaaataaatg ctattgaaaa agaaagaaaa
     4201 ttaaaagagg aagtaataga tgttactcaa ccaggaaaag ttttaaaggt tgggaagaaa
     4261 catccaataa ctcaaattat agatgaagta acagatatat ttatcggaat gggattctct
     4321 atagcagaag ggccagaagt tgagactgtt gaaaacaact ttgatgcatt aaacgctcct
     4381 aaagaccatc catcaagaga tatgagcgac acattctata tcaatgatgg ggtattactt
     4441 agaactcaaa catctccagt tcaagtaaga actatgagaa gtcaagagtt accaataaaa
     4501 gtaattgcac caggtagatg ttttaggtca gattcgccag atgctacaca ctcaccaatg
     4561 ttccatcaga tagaagggct tgttgttgga aaagatgtta ctatggcaga atttaaagga
     4621 actatggata tcttcgttga aaaattgttt ggttctgata tcaaaactaa gtttagacct
     4681 cacaacttcc catttacaga accaagtgca gaggttgatg ttacttgttt caaatgtggt
     4741 ggtaaaggtt gcccaatgtg taaatatgaa ggttggatag aaatattagg ttcaggtatg
     4801 gttcatccaa acgtgcttag aaattgtgga atagacccag aagtttacag tggatttgca
     4861 tttggagttg gggttgaaag acttgcaatg cttaaatacg aaatagatga tattagatta
     4921 ttattcgaaa atgatatgag attcttaaat caattttaat taggagggta tatagatgtt
     4981 agtatcttta aaatggctta gagactatgt tgatatagac atggatgtaa aagagttcgc
     5041 tgataaaatg acaatgacag gaactaaagt tgaaacaata gattattatg gtgaagaaat
     5101 agaaaatata ttggttggaa agattttaga aataaaacaa catccaaatg ctgataagtt
     5161 ggttgtaact aaagtagata ttggagataa agttgttcaa atagttacag gagctacaaa
     5221 tatatcagaa ggagattata ttccagtagc tataaatggt tctaagttac ctggaggagt
     5281 tgaaatcaaa cagactgatt tcagaggaga attatcagat ggtatgatgt gttcagcagc
     5341 tgaactaggt atagatgaac attacattga agagtataaa agaggtggta tatatatttt
     5401 agaccacgaa gattcttatg aattaggaaa agatataaaa gatgttttag gattaaaaga
     5461 tgctttaata gattttgaat taacttcaaa cagacctgat tgtaaatgta tgatgggtat
     5521 agctagagaa gcagctgcaa ctataggaac aaaggtaaaa tatcctgaaa tcgaagtaaa
     5581 agaaagtgat gaagagatag acttcaaagt tgaaatagac aatccagatt tatgtagaag
     5641 atatgttgct agaatggtta cagatgtaaa aatagaacct tctccatatt ggatgcaaag
     5701 aagacttaca gaagcaggag taagacctat aagtaacata gtcgatataa caaacttcgt
     5761 aatgttagag cttggtcaac cacttcatgc ttttgatata aatcaagtag agactggaag
     5821 aatagtagta agaaatgcta aagatggaga gaaacttgta acattagatg atgttgagag
     5881 aacattagac aaagatatgc tagttataac aaatggagaa aaatcacttg gtttagctgg
     5941 tgtaatgggt ggtgctaact cagaaataac ttctaatacg aagactgtac tttttgaaag
     6001 tgccaatttc aaaccagaaa acataagaat gacagctaaa aaagttggta ttaggtcaga
     6061 agcatcttca agaaacgaaa aagatttaga ccctaatctt gcagagatag cagcaaatag
     6121 agctgtacaa cttgtcgaaa tgttaggagc aggaaaagtt ttaaaaggtg ttgtagatgt
     6181 atatccaaat aaaccagaac ctaaaaaatt ggtagtaaat cctcaaagaa ttaatcattt
     6241 attaggtgta gatgtaccaa tggagcagtt tgtaggaatt ttagaatcat tagagtttaa
     6301 atgtaatttg gtagctgatg ataaattaga aatagatgta ccaagcttta gaacagatat
     6361 ggaacaagaa gctgatgtat gggaagaaat agctagaatt tatgggtttg agaatatacc
     6421 ttctgtacag ttagaaggaa atacgacagc aggaattaaa acttcaaaac aaaaattcat
     6481 ggatgcctta aaagataatt caactgctgt aggattaaac gaaatattaa catattcatt
     6541 tgtaagccct aagggagtag ataagataag agtaccagaa ggtaatgcaa agagaaattt
     6601 tgttaaatta cttaacccat taggagaaga aacttctgta atgagaacta ctttaatacc
     6661 aaatatgtta gatgttttat caactaatgt atctcataaa atagaagaag tatctgcatt
     6721 tgagtgtgga catatattca tcccacaaga ttcagaatta cctaaggaag aaaatagaat
     6781 gtgtgtaggt atgtacggta aagatgttga cttctttgca ctaaaaggaa ctatagaaac
     6841 tatacttgta aatgtcggat ttaaaggtta tgaaattgaa cctcaagata ataatactac
     6901 cttccaccct ggtagatgtg ctaaaatagt gtataataat aaatatgtag gtacattagg
     6961 tgaattgcat ccagatgtta tagaaaacta caatctaggt caaagagttt atgtcgctga
     7021 aatagatatt gactttgtat ttgataattc tgatagaaca aagaattatg tgccattacc
     7081 aaaatatcca tctacatcaa gagatatagc tcttattgtt aaagatgatg tatttgtaaa
     7141 acaaatagaa gatataataa aggaaaatgg tcaaggatta gtagaaagct acaagttatt
     7201 tgatgtttac aaaggttctc aaatagaagc tggatacaag tcaattgcat attcaataac
     7261 ttatagaagt aaagataaaa ctctaacaga tgaagatgta gctaaggtgc atgataagat
     7321 attaagtgaa ttaagcgaaa aattaaatgc taacttaaga tcaaattaac aactaatgtc
     7381 gggggaggtt aggatgaaca aagtaatggt taaaatccat ggcgctgaat atccaatggt
     7441 tggagacaaa tcagaaaagt ttatgattag tattgctgat tttgtagaca aagaaatgga
     7501 taaaataaca cgtcaaaacc cgaaattgag cttatcagtt gctgcgatat taactgcatt
     7561 aaatatatca gaccttttat ttgaatgttc agatgagaat gagaagttga ttaaagctaa
     7621 tgaagaattg agtaagaagg ttggagcatc taatgaagag cttcaattag aaatcaagtc
     7681 tttaaagctt acgatagcag aaaaagaagc agaaaatcgt gaagctgaaa ctaagatgaa
     7741 agagttaatt gagattatag aaaataaaaa gcaagaaata ttcgaactta gcaatacgac
     7801 agaaggttct agagcagagt tagatgctta taaaaacaag attgaggagt taagtactca
     7861 acttgaagaa gcaaatgaaa gagctactat agctgaaaac ttagcttctg agtttcaaaa
     7921 caaagcttat gatttacagt taaaatgtac agggttaaat aatgatgcaa aaaatgtgga
     7981 ataaactaaa agatttgctg tctcttgttt tttgagacag cttcttttaa tgtataaata
     8041 taaatgctat ataaatatag ataatgttta aatgtaaata aatataaatg
//
LOCUS       DAEPXF010000168         8037 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain HB27
            SAMN13376389-rid12479423.denovo.175, whole genome shotgun sequence.
ACCESSION   DAEPXF010000168 DAEPXF010000000
VERSION     DAEPXF010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13376389
            Sequence Read Archive: SRR10518432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8037)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8037)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001069624.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001069624.1
            Genome Coverage       :: 315x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/15/2021 01:54:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,165
            CDSs (total)                      :: 4,076
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 89
            rRNAs                             :: 10, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 10 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 19 of 69
            Pseudo Genes (incomplete)         :: 44 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 10 of 69
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8037
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13376389-rid12479423.denovo.175"
                     /strain="HB27"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="China: Hebei"
                     /collection_date="2017"
     gene            complement(146..1813)
                     /gene="bclA2"
                     /locus_tag="KRL67_003123"
     CDS             complement(146..1813)
                     /gene="bclA2"
                     /locus_tag="KRL67_003123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-like exosporium glycoprotein BclA2"
                     /protein_id="HBH1523986.1"
                     /translation="MSDISGPSLYQDIGPTGPTGPTGPTGPRGATGATGANGITGPTG
                     NTGATGANGITGPTGNMGATGANGTTGSTGPTGNTGATGANGITGPTGATGATGANGI
                     TGPTGNKGATGANGITGSTGPTGNTGATGANGITGPTGNTGATGATGATGLTGATGAT
                     GANGITGPTGNTGATGANGVTGATGPIGNTGATGSTGSIGATGANGVTGATGPIGATG
                     PTGADGEVGPTGAVGATGPDGAIGPTGNTGATGATGANGLVGPTGATGATGANGLVGP
                     TGPTGATGPDGAIGPTGAVGATGPTGADGAVGPTGATGATGANGATGPTGAVGATGAN
                     GAAGPIGPTGPTGANGVAGATGATGATGANGATGPTGAVGATGANGVAGAIGPTGATG
                     ANGATGATGATGATGANGATGPTGATGATGVLAANNAQFTVSSSSLGNNTLLTFNSSF
                     INGTNITFPTSSTINLAVGGIYNVSFGIRAILSLAGFMSITTNFNGVAQNNFIAKAVN
                     TLTSSDVSVSLSFLVDARAAAVTLSFTFGSGTTGTSPAGYVSVYRIQ"
     gene            complement(1992..2531)
                     /gene="hpt"
                     /locus_tag="KRL67_003124"
     CDS             complement(1992..2531)
                     /gene="hpt"
                     /locus_tag="KRL67_003124"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HBH1523987.1"
                     /translation="MDIETKKWEVLYSEEKIKDKLRELGAIIEKDYKDKNLMVVSLLK
                     GSFIFCADLVRNINLPLRVNFMTTSSYGNNEESTGRVKVVSDVTTDIAGYDVLVVDDI
                     TDSAFTMDFVLKHLKAKNPASLKCCVLLDKPSRRKVDLVPDYCGFEIEDKFVVGYGFD
                     FGDYYRNVPYIFNVTDEDR"
     gene            complement(2859..4166)
                     /locus_tag="KRL67_003125"
     CDS             complement(2859..4166)
                     /locus_tag="KRL67_003125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine dehydratase subunit alpha family protein"
                     /protein_id="HBH1523988.1"
                     /translation="MRDIRKELVEMLKAEVKPAVGCTEPVALALACAKAKELLGEEIV
                     ENRMLVSPSIYKNGMCVGIPGTERLGLKIAAALGIVGGHSENGLSVLETLTKEEVKIA
                     EDYMDNTPLSITPADTREKVFIEVVLKGKNHIAKVRIRTKHDNFTFLEKDGEVLLDNE
                     PKVSASNDVAEKAESLMDTVTIQELIKNVEEIDFKDIEFLLDGVKMNEEMAEYGLKQK
                     TGIGVGYGIKKSIEEGLLGNDVINYAMMLTAGASDARMAGVKMPVMSSNGSGNHGLTA
                     ILPIVAYNKKFPQSDERLAKALAISHLVTGYIKNYTGRLSAVCGCGVAASTGATAGIS
                     WLMNGTENQIEGAIENMIADLSGMICDGAKAGCALKLSSAASAAIQSAIIAKQDCFVP
                     PLNGIVGSSVEQSIQNLGRVSDKGMSITDEIILNVMNDMNKVD"
     gene            complement(4578..6353)
                     /locus_tag="KRL67_003126"
     CDS             complement(4578..6353)
                     /locus_tag="KRL67_003126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBH1523989.1"
                     /translation="MMIMIGLEYIKSDIQNIAEAIVSVLNIDVTIVNKELFRIAGTGV
                     YIDKIGEKVDKYTAFKKSLTEQITILIDDPKSSDICRECYKSGACVEFAEVCCPIICD
                     GDSHGVIGLIAFTKEQAQIIENNKSGLINFLGKMADLISNKLKAQIKTYELELEKKKL
                     ETLLNNMDKAVVSIDVDGKIDKYNSKFKELFNIKDSITGKNIFVELDFIKKPSINNFK
                     KDKSCSFFYKRYGYDSKGIYNINKIVLKNELKGYVIDFIDKRDAIKNYNKMNKDYRIK
                     LENIIGESVAIRNSKKEALIASKSTSTVLITGESGTGKELFARAIHNHSNRVDNPFIA
                     VNCAAIPDNLLESELFGYEEGAFTGAKKGGKLGMFEVAHKGSIFLDEIGDMSLHLQSK
                     LLRVLQEKELNKIGSKSNVSIDVRIIAATNKDLEKMVQNGTFREDLYYRLNVIPIKLP
                     SLRERKEDIPLIIKHMVKEYSKKLDKNVIDIDSRVVDAMMEYKWPGNIRELQNIIEYS
                     VNMSSSSIITMNVIPQKIKNTSVEELEVKSDRIIPLDELEKIEIIKALNKYKDYKKDK
                     ELTAKALGISRATLYRKIEKYNIIS"
     gene            complement(6350..6487)
                     /locus_tag="KRL67_003127"
     CDS             complement(6350..6487)
                     /locus_tag="KRL67_003127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH1523990.1"
                     /translation="MEINYELIIIVMLFVILMSIQFTLNKIYLILKEIRQILMLKKNK
                     E"
     gene            complement(6565..7134)
                     /locus_tag="KRL67_003128"
     CDS             complement(6565..7134)
                     /locus_tag="KRL67_003128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HBH1523991.1"
                     /translation="MKSILCQINKQPVKAKYTSDELECFLDTIEYNVGDKILLVGHIG
                     NLGKRLRGLGTSVTILENSNYEDVCYSLVYNENCNVVKGKLEFLPFDDNQFNKIIIVD
                     QFNVITDCEKASSEIQRVLKGNGEVILEDLNLKNLKVKLNYLKHKFCGSVAKIHYPHE
                     VFNIFSRLNFDGMIKESNKLKYIYVGKRR"
     gene            complement(7318..7953)
                     /locus_tag="KRL67_003129"
     CDS             complement(7318..7953)
                     /locus_tag="KRL67_003129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HBH1523992.1"
                     /translation="MIAVKDENNVVNLRGELDNKLEFSHEIFGEKFYNMKIKINRLSD
                     SFDILPMTVSERLLQDIDLDKQNLVNVVGQLRSYNKTLNNKNRLVLTVFVREIKSIDE
                     ENKDPNSIFLDGYVCKEPVYRKTPLGREITDLLVAINRPYNKSDYIPSIVWGRNAKFA
                     KNLKVGDRIQLWGRVQSREYEKKIDENNVVKKMAYEVSISKIKKLDENNNE"
BASE COUNT         2278 a         1623 c          935 g         3201 t
ORIGIN      
        1 aatattatct tatttattgg tatatggaat catattgttt attattttat aatcttttca
       61 tcatataaac ctattaaata ttatcttatt tattggtata tggaattatt ttgtttatta
      121 ttttataatt ttttcatcat ataaactatt gtattctata aactgataca tatccagctg
      181 gagaagtacc tgtcgtgcct gaaccaaatg taaaacttaa agtaacagct gctgctctag
      241 catcaactaa aaaacttaaa cttacgctta catctgatga agtaagcgta tttactgctt
      301 ttgcaataaa gttattttga gctactccat taaagttagt agtaattgac ataaatcctg
      361 caagcgaaag tatggcacgt ataccgaaag atacattgta tatccctcca actgcaagat
      421 ttatagtact acttgttgga aaagttatat tagttccatt tataaatgat gaattgaatg
      481 tcaataatgt attattccct aaacttgaag aagatactgt aaattgtgca ttgtttgctg
      541 ctaacactcc tgttgctcca gtcgctccgg ttggacctgt tgctccattt gctcccgttg
      601 ctccggtcgc ccctgttgct ccggttgctc catttgctcc cgttgctccg gttgggccta
      661 tcgctcctgc tactccattt gctcccgttg ctcctactgc tccggttggg cctgttgctc
      721 catttgcccc tgttgctcct gttgctcctg tagctcctgc tactccattt gctccggttg
      781 gacccgttgg acctattgga cctgccgctc catttgctcc cgttgctcct accgctccgg
      841 ttgggcctgt tgctccattt gcccctgttg ctcctgtcgc tccagttgga cctactgctc
      901 catctgctcc ggttgggcct gttgctccta ctgctccggt tggacctatt gccccatctg
      961 gacctgttgc tccagttgga cctgttgggc ctaccaaacc atttgctccc gttgctccgg
     1021 ttgctccggt tgggcctacc aaaccatttg ctcccgttgc tccggttgct cctgtatttc
     1081 cggttggacc tattgctcca tctggacctg ttgctcctac tgctccggtt ggacctacct
     1141 ctccatctgc tccggttgga cctgttgctc ctattggacc tgtagcacct gttactccat
     1201 ttgctcctgt tgctcctata cttccggttg accctgttgc tcctgtattt cctattggtc
     1261 ctgtagcacc tgttactcca tttgctccag tcgctcctgt atttcctgtt ggacctgtta
     1321 ttccatttgc tcctgttgct ccggttgctc ctgttagtcc ggttgcccca gttgctccgg
     1381 ttgctcctgt atttcctgtt ggacctgtta ttccatttgc tccagtcgct cctgtatttc
     1441 ctgttggacc tgtagaacct gttattccat ttgctcctgt tgctcccttg tttcctgttg
     1501 gacctgttat tccatttgct cccgttgctc cggttgctcc tgtcggacct gttattccat
     1561 ttgctccagt cgctcctgta tttcctgttg gacctgtaga acctgttgtt ccatttgctc
     1621 cagtcgctcc catatttcct gttggacctg ttattccatt cgctccggtt gctcccgtat
     1681 ttcctgttgg acctgttatt ccatttgctc ccgttgctcc ggttgcgcct ctaggtccag
     1741 tagggcctgt tggccctgtt ggccctgttg gacctatatc ttgatataaa cttggacctg
     1801 aaatatcact catataatta atcctccttt tttaaagtta gagtaatact ctataaatat
     1861 tgagtattac tctatataat tatatgaatc gacaatttct tatgtgataa tgtatctatt
     1921 tgcttatata aaaaaacctc aacacgatta ataaaaatca tattaaggca tccacaaaat
     1981 tatataatag cttatctatc ttcatcagta acattgaata tataaggaac atttctatag
     2041 taatcaccaa aatcaaaccc atatccaacc acaaacttat cttctatttc aaatccacaa
     2101 taatctggca ctaaatcgac ttttcttctg cttggtttat ccaaaagaac acagcacttt
     2161 aagctagcag gatttttagc ttttagatgt tttaacacaa aatccatagt aaatgcagaa
     2221 tcagtaatat cgtctacaac caaaacatca tatcctgcta tatcagtagt cacatctgaa
     2281 actactttaa cccttccagt actttcttca ttgtttccat aactagatgt agtcataaaa
     2341 ttaactctaa gtggtaaatt tatatttcta actaagtctg cacaaaatat aaagctacct
     2401 tttaatagag aaactaccat aagattttta tctttataat ctttttctat aattgctcct
     2461 aattctctta atttatcttt aattttttct tctgaataca atacttccca tttttttgtt
     2521 tctatatcca taaaaagacc tccaattgta ataatccttc ttaattttat aagagattgc
     2581 tcactttttc aatactctta ttcaatatta gtcaatatta ttgagttttt atgttcattc
     2641 aacaattatt aacaatgtat cttatatttt aagtagttca tatatccaca ctaaattaca
     2701 tactatatat tttaaacaaa aaaagaaaaa acttgcacta acaagttttt tctattatac
     2761 ataaatttat acataatcat attttattag agggtactaa ttaaatttat ttatgatatt
     2821 tttgttatac ctcataatac aaaacatatt ataaagtact agtcaacttt attcatatca
     2881 ttcattacat ttaagattat ctcatctgtt atagacatac ctttatcact tactctacct
     2941 aagttttgta tactttgctc aacacttgaa ccaactatac catttagtgg tggtacaaaa
     3001 caatcttgct tagctattat agcactttgt atagctgcag atgctgcaga agataatttt
     3061 aatgcacaac cagctttcgc accgtcacat atcattccac ttaagtcagc tatcatattt
     3121 tctatagctc cttctatttg attttcagta ccattcataa gccaagagat accagcagtt
     3181 gctccagtag atgcagcaac accacatcca catacagcag ataaccttcc tgtataattc
     3241 ttaatataac cagttactag atgagatata gctaaagctt ttgctagtct ttcatcagat
     3301 tgtgggaatt ttttattata agcaacgatt ggaagaattg ctgttaatcc atggtttcca
     3361 cttccatttg aactcataac aggcattttt acccctgcca ttcttgcatc tgaagctcca
     3421 gcagttaaca tcattgcata atttataaca tcattaccta aaagaccttc ttctatagac
     3481 tttttaattc catatccgac accaatacca gttttttgtt ttaatccata ttcagccatc
     3541 tcttcattca tttttactcc atctagtaaa aattctatat ctttaaaatc aatttcttct
     3601 acatttttta ttaattcttg tatagttact gtatccatta aactttcagc tttttctgct
     3661 acatcatttg aagctgatac ttttggttca ttgtctaata atacttctcc atctttttct
     3721 aaaaacgtaa aattatcatg tttagttctt attctcactt tagcaatatg gttttttcct
     3781 tttaacacaa cttctataaa tactttctct cttgtatcag caggtgttat tgatagtgga
     3841 gtattatcca tataatcctc tgctatttta acttcctctt ttgttagagt ttctaacaca
     3901 cttaatccat tttcagaatg tccacctact atacctagag ctgctgcaat ttttaatcct
     3961 aatctttcag tacctggtat acctacgcac ataccatttt tatagatact aggactaact
     4021 aacatacgat tttctactat ttcttctcct aataattcct ttgcctttgc acatgctaga
     4081 gctaaagcta ctggttcagt acaccctact gctggtttaa cttctgcctt taacatttcc
     4141 actaattctt ttcttatatc tctcattata tataaacctc ccaattaagt ttagatttag
     4201 tactcaaata attaattaaa taatttctta tctatattta tagcaaaata catgccatat
     4261 tttttatatc tttttatatt tttagtctta tattctataa ccattggtat gactgcttct
     4321 atttatgtat tttttttatt tgcatagagt atttatattc tttaaaacgg aaaaacatat
     4381 gacaatttcg caaaaagaca aaaaaaagtc tcaaaatgag attctatatc attttgagac
     4441 ggctcaaacg tattactttt taatttataa aaaccatttt ttaattcata aaaaaagatt
     4501 atgcaaaaca cttatagcag aagtttaaag ttatgaattt agaaaattta aagtttatat
     4561 atctttttat tatattacta tgaaattata ttgtattttt caatttttct atataatgta
     4621 gctcttgata ttccaagtgc tttagctgtt aattctttat cttttttata atctttgtac
     4681 ttatttaaag cttttattat ctcaatcttc tccagttcat caagtggaat tatcctatcg
     4741 cttttaactt ctaactcttc tacagaggta ttcttaattt tttgaggaat tacattcatt
     4801 gtgattattg aagaagaaga catattcaca ctatattcaa taatattctg caactctctt
     4861 atattccctg gccacttata ttccatcata gcatcaacaa ccctgctatc tatgtcaata
     4921 acatttttat ctaatttttt tgaatactct ttaaccatat gcttgattat aagtgggata
     4981 tcttcttttc tctctcttaa acttggtaat tttataggta taacatttaa tctataatat
     5041 aaatcttccc taaaggtgcc attttgaacc attttttcta aatctttatt tgtagccgca
     5101 ataattctta catctattga aacatttgat ttacttccaa ttttattaag ttccttttct
     5161 tgtaatactc tcaataactt tgactgtaaa tgcaaactca tatctcctat ttcatctaaa
     5221 aatatacttc ccttatgtgc tacttcaaac attccaagct ttccaccttt tttagctcca
     5281 gtaaaagctc cctcttcata tccaaataat tcactctcta ataaattgtc tggtatagca
     5341 gcacagttta cagctataaa aggattatct accctattgc tatggttgtg tatggctctt
     5401 gcaaatagct ccttacccgt tccactttcc ccagtaataa gtactgttga tgttgattta
     5461 gaagctatta aggcttcttt tttagaattt cttatagcta cactttctcc aattatgttt
     5521 tccaatttta ttctataatc tttattcatc ttattgtagt tttttatagc atctctttta
     5581 tctataaaat caataacata tccctttaac tcatttttta atactatttt attaatgttg
     5641 tatattccct tcgaatcata tccgtacctt ttataaaaaa aactacagga tttatccttc
     5701 ttaaagttat ttatagaagg tttctttata aaatctaatt cgacaaatat gtttttgcca
     5761 gttatactat cttttatatt gaaaagctct ttaaattttg aattatattt atcaattttt
     5821 ccatccacat caatagacac aacagctttg tccatattgt ttaatagtgt ctctaatttt
     5881 ttcttttcta attcaagctc ataagtttta atttgagctt ttaatttatt agatattaag
     5941 tctgccattt ttcctaaaaa gtttataaga ccacttttat tattttctat aatttgtgct
     6001 tgttcttttg taaaggctat aaggcctata acaccatgag aatcgccatc acatataata
     6061 ggacaacaca cctctgcaaa ctctacacaa gctccacttt tatagcattc cctacatata
     6121 tcactactct ttgggtcatc aattagtatt gtaatctgtt ctgtaagtga ttttttaaaa
     6181 gccgtatatt tatctacttt ctcacctatt ttgtctatat atactcctgt acctgcaatc
     6241 ctaaataact ccttatttac tatagttaca tctatattga gtactgatac tatagcttca
     6301 gctatatttt gaatatcaga ttttatatat tctaaaccta tcataatcat cattccttat
     6361 ttttctttaa cataagtatt tgtcttattt ctttaagaat caagtatatc ttatttaaag
     6421 taaattggat tgacattaga attacaaaga gcataacaat tattattaat tcataattta
     6481 tttccatcta aacatctcct tataacaaaa gactaggctt aacctagtct tttatattat
     6541 atccatattt atattttaac tcatttatct tctttttccc acataaatat attttaattt
     6601 attcgactct ttaatcatac cgtcaaagtt taatcttgaa aatatattaa atacttcatg
     6661 tggatagtga atctttgcaa cagaaccaca aaatttatgt ttaagatagt ttaacttaac
     6721 ttttaaattc tttaaattta aatcctctaa tataacctct ccattgccct ttaacactct
     6781 ttgtatttca gatgaagctt tttcacaatc tgtaataaca ttaaattggt caactattat
     6841 tatcttgttg aactgattat catcaaatgg tagaaactct aacttacctt taactacatt
     6901 gcaattttca ttatacacta gagaataaca gacatcttca taattactat tctccagtat
     6961 agttacagaa gttccaagac ctctcaatct cttgcctagg tttcctatat gaccaacaag
     7021 caatatttta tcacctacat tatactcaat tgtatccaaa aaacattcta attcatcaga
     7081 tgtatattta gctttaactg gctgtttatt tatctgacat aatatgcttt tcacaatgct
     7141 tcacctcttc atttcctcaa ttataaatta tgtacagatt acattataat aaatggcgag
     7201 caactattca agagatattt cattagtatt tttttaaaca atctttatac attttaatat
     7261 gatattatac caaatttatt aggattttat tcataaaaat cagtctattt tttatgttta
     7321 ttcattgtta ttttcatcca atttctttat tttcgatatg gatacttcat atgccatttt
     7381 tttgaccaca ttattttcat ctattttttt ctcatattct ctactttgaa ccctacccca
     7441 aagctgtatt ctatcaccaa cctttagatt ctttgcaaat ttagcatttc taccccaaac
     7501 tattgatggt atataatcag atttgttata aggtctgttt atagcaacta ataaatcggt
     7561 tatttccctt cctaaaggtg tcttcctata cacaggttcc ttacatacat atccatctaa
     7621 aaatatacta ttagggtctt tattttcctc atctatgctt tttatttctc tgacaaacac
     7681 tgtaagcact aatctatttt tattatttaa tgttttgtta tatgatctta attggcctac
     7741 gacattgact aaattttgtt tatcaaggtc aatatcttgg agaagtcttt ctgatacagt
     7801 cattggaagt atatcaaaag aatcgcttag tctgtttatt tttattttca tattataaaa
     7861 tttttctcca aatatctcat ggctaaattc tagcttattg tctaactccc ctcttaagtt
     7921 tactacgtta ttctcatctt taacagctat catattttat ccctccacaa ttgttaattc
     7981 ttatatttct ggtatcagct tatataataa atatatttat aagggatgta ttttatt
//
LOCUS       DAEPXF010000169         7956 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain HB27
            SAMN13376389-rid12479423.denovo.176, whole genome shotgun sequence.
ACCESSION   DAEPXF010000169 DAEPXF010000000
VERSION     DAEPXF010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13376389
            Sequence Read Archive: SRR10518432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7956)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7956)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001069624.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001069624.1
            Genome Coverage       :: 315x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/15/2021 01:54:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,165
            CDSs (total)                      :: 4,076
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 89
            rRNAs                             :: 10, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 10 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 19 of 69
            Pseudo Genes (incomplete)         :: 44 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 10 of 69
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7956
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13376389-rid12479423.denovo.176"
                     /strain="HB27"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="China: Hebei"
                     /collection_date="2017"
     gene            complement(4..717)
                     /locus_tag="KRL67_003130"
     CDS             complement(4..717)
                     /locus_tag="KRL67_003130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2140 domain-containing protein"
                     /protein_id="HBH1523993.1"
                     /translation="MGKIAKILLSLIVIVLVLASIVVYILTPKERYDVSSQNNKIIDE
                     EYLSKGSYINSMEVVKNPLRMVGKISVSEEEFKNLIYTLMQKHGINELENNFVEMKNG
                     KIKVAGPYKVFGLVNSQYELELRPNLKDGNLVVSLENVKLGKFKLSDKMLEKILSNYN
                     KKVPFEVNGNKITLEKSYLYPVTLKNISIKRGNVDLDMEVEIDLKSQINGALGYLKDS
                     GKVQDILNHLINLKKTSVG"
     gene            1009..2673
                     /locus_tag="KRL67_003131"
     CDS             1009..2673
                     /locus_tag="KRL67_003131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase/YqeY domain fusion
                     protein"
                     /protein_id="HBH1523994.1"
                     /translation="MSNETNSSNFIKNIIINDLETGKHDSIITRFPPEPNGYLHIGHA
                     KSICLNFGLAKEFNGKANLRFDDTNPLKEDVEYVESIKEDVKWLGFDWNELNFASNYF
                     DEMYKRALILIKKGKAYVCDLTQEEMREYRGTLTEPGKESPHRNRTIEENLDLFERMK
                     NGEFKDGEKTLRAKIDMSSPNINLRDPIIYRISHSTHHNTGDKWCIYPMYAFAHPIED
                     AIEGITHSICTLEFEDQRPLYDWFVKECEMENIPRQIEFARLNINNTVMSKRKLKQLV
                     DEGIVDGWDDPRVPTISGIRRKGYTAEALRNFCSEIGVSKVNSTVDSQMLDYFLRENL
                     QPKAPLTMGVLRPLKLIITNYPEDKIEMLEIENNAKDESQGKRLVPFSRELYIEQDDF
                     MEEPVKKYFRFFPGNEVRLKGAYFVKCTDVIKDENGNVVEIHGTYDPETKSGSGFTGR
                     KVKSTIHWVDAKSAIPCEFRLFEPLILDDIPENEGKHFLEQINPNSLEILQGFVEPTQ
                     IKDAKPFDKFQFVRNGFFSIDNKYTTDEKFVFNRIVPLKSSFKPGK"
     gene            complement(2780..3679)
                     /locus_tag="KRL67_003132"
     CDS             complement(2780..3679)
                     /locus_tag="KRL67_003132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegS/Rv2252/BmrU family lipid kinase"
                     /protein_id="HBH1523995.1"
                     /translation="MKKVKLIYNPNSGEKKILSNLDTIIELYQEKNYLLIPYRLTIKE
                     PVKNAFKEVDNSYEHILIAGGDGTVDLVVNAMKELDIKIPIGILPTGTANDFSNALQI
                     SFDIKEAINEIINSKPKKIDIGKVNNKYFINVASAGMFTDVSQRINTDLKNSFGRISY
                     YIKGIEEALYMRKFKIKVSSEEMYYDGDMYLMLIFNGKTAGNINLAYKAEIDDGYLDV
                     IIFKGMPIPKSIPVLISVLRGDYLDQYNGNEILYFKTKKVHIECEDSLITDIDGEKGP
                     DFPLNIECIKDGIEVMGICSEKS"
     gene            complement(3706..4344)
                     /locus_tag="KRL67_003133"
     CDS             complement(3706..4344)
                     /locus_tag="KRL67_003133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003356606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein"
                     /protein_id="HBH1523996.1"
                     /translation="MDQNLNWLNEKRIEKTIKSLENNNMKGYLVSNKEELLKKIEEIV
                     KVGSLVGCGGSMTLFETGVIDFIREKYNFLDRYAKGIKPEDMKEIYRKSFSADAYFTS
                     TNAITEDGELYNLDGNGNRVAAMIYGPDKVIVIAGVNKIVKDVDEAIKRTKTIAAPAN
                     CKRLSTNTGCKTTGICIDCSSPGRICCSYTLIKRQLTPERIHVIFLNEDFGY"
     gene            complement(4685..5683)
                     /locus_tag="KRL67_003134"
     CDS             complement(4685..5683)
                     /locus_tag="KRL67_003134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4003 domain-containing protein"
                     /protein_id="HBH1523997.1"
                     /translation="MEQSFINGKVNLLIENYKALNEVKGSWQMGLIQHSCALAFTLKN
                     KRISPRLVEERIELIKKNTGLFSNFRGYNIFYIATLLSFESNPESSFKMILDIYKELK
                     SEKFWGDTYLPLTASIVYENREKMDYLTCISKMKIIYDYMRKKHPFITSSDDYCNIAL
                     IAIHSKNLDEDLEYIEKCYEFLNENGFYKGNDLQALSQILLFSDDRTMLKCKKTIELK
                     KAFKENDCKMNYYGYPIIGAISLLDYREDEIIEEIKNVSNKLKEEKGFGNWSLGKSNR
                     VMISSAIVASIYADLIRNENNVGSITNNIFLNIVVAIQIACVMAATSAAIASSSSN"
     gene            complement(5781..6356)
                     /locus_tag="KRL67_003135"
     CDS             complement(5781..6356)
                     /locus_tag="KRL67_003135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH1523998.1"
                     /translation="MKLKEALITCVVCSVCLTPLLVNGETKIDADHCKESIVNIDTIV
                     KSGKSFNSKYNQGMEDYKNSDEYKLKVQKEKLQNQLGIKINKLVPITFEVSYYTSLNQ
                     ENGYGAITATGDDLEDGFVANNHLSFGTKIIVDGKLKTVMDRGSSKYFSNSNAIDVFV
                     PKWSNESESEYYDRVNNMGRHYKKGYIIVEE"
     gene            6672..6935
                     /locus_tag="KRL67_003136"
     CDS             6672..6935
                     /locus_tag="KRL67_003136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH1523999.1"
                     /translation="MIKNTKQITVEVNEILLQEISKITNDENNILTDLINESIVNYIL
                     ENKKHNIENKDLEEVIKFYKKENTEKPKSAARAIAEAKTHTLY"
     gene            complement(7372..7749)
                     /locus_tag="KRL67_003137"
     CDS             complement(7372..7749)
                     /locus_tag="KRL67_003137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HBH1524000.1"
                     /translation="MTITKIPPAELKVMKFIWSSDFTVASKDVIEAMEKLYNWKQTTT
                     LTLLSRLVNKQFLSARKIDRHTHYTVLIGEEEYLHFETKTFLDNMHGSSIESFMKSLF
                     KKGVNEEELNSVEKWVKDIKKDE"
BASE COUNT         2804 a         1230 c          841 g         3081 t
ORIGIN      
        1 attttaacca acagatgttt tcttcaaatt aatcagatga tttagtatat cttgaacctt
       61 tccagagtcc tttaaatatc ctaatgcccc atttatttgt gattttaaat ctatttctac
      121 ttccatatcc aaatcaacat ttcctctttt tatagatata tttttaagtg ttactgggta
      181 aagataactt ttttccaaag taatcttatt tccattaacc tcaaatggaa cttttttatt
      241 gtagttactt aatatttttt ctaacatttt atcacttagc ttaaattttc caagtttcac
      301 attctcaaga cttacaacta agtttccatc ctttaaatta ggtctcagtt caagttcata
      361 ttgactattt accaaaccaa ataccttata tggtcctgct acctttattt taccattttt
      421 catttcaaca aaattatttt caagctcatt aatgccgtgc ttctgcatca atgtatatat
      481 taaattttta aactcttcct ctgaaacaga tatttttcca accattctaa gtggattttt
      541 tactacttcc atagaattta tatatgagcc ttttgataaa tattcttcat ctatgatttt
      601 attattttgt gaactaacat catatctttc ttttggtgtc aagatatata caacaatact
      661 tgcaagtact aaaacaataa caattaaact taacaaaatc tttgcaatct ttcccataat
      721 cgtctccttt gcaaatctat cagcagattt aaaataaatt tagataacta acttcacata
      781 atattcttaa caatatacat actataatac agtcttttaa aaattaattc aaccaatctc
      841 attttttgta aataaactgt aaaaacatgt ttttagtata aaagttaata ttttgcataa
      901 tactcttaat tacaccttta tttttttcta atcttggatt aaaattaata ttatgcaata
      961 cagtgtagtg ttctgtatta taatcaaatt aaataggagg tatagaatat gtcaaatgaa
     1021 acgaactcat ctaactttat aaaaaatatt attataaacg accttgaaac aggaaaacat
     1081 gacagtatca taactcgttt tccacctgag ccaaacggat atttacatat tggtcatgca
     1141 aaaagtatat gtcttaattt tggattagct aaggagttta atggaaaagc taatttaaga
     1201 tttgatgata caaacccatt aaaagaggat gttgaatatg tagaatccat aaaagaagat
     1261 gtcaagtggt tagggtttga ttggaacgag ttaaattttg catctaacta ctttgatgaa
     1321 atgtataaga gagctttaat actaataaaa aaaggtaaag cttatgtatg tgatttgaca
     1381 caagaggaaa tgagagaata tcgtggtact ctaactgaac caggaaaaga aagtcctcat
     1441 agaaacagaa ctattgaaga aaaccttgat ttatttgaaa gaatgaaaaa tggtgaattt
     1501 aaagatggtg aaaaaacatt aagagccaaa atagatatgt cttctccaaa tataaacctt
     1561 agagacccaa ttatatatag aatatctcat tcaacacacc acaatacagg agataaatgg
     1621 tgcatatatc ctatgtatgc ttttgcccat ccaattgaag atgcaattga aggaataact
     1681 cactctatat gtactttaga gtttgaagac caaagacctc tttacgattg gtttgttaaa
     1741 gaatgtgaga tggaaaatat acctagacaa atagaatttg ctagacttaa tataaataat
     1801 actgtcatga gtaaaaggaa attaaaacaa cttgtagatg agggtattgt tgatggttgg
     1861 gatgaccctc gtgttcctac aatatccgga attagacgta agggatatac tgcagaagca
     1921 ttgcgcaatt tctgtagtga aataggggta tcaaaagtta actctacagt agatagtcaa
     1981 atgcttgatt atttcctaag agaaaactta caaccaaagg ctccccttac tatgggagtt
     2041 ttaagacctt taaaacttat tataaccaat tacccagaag ataagattga gatgcttgaa
     2101 atagagaata atgctaaaga tgaatctcaa ggaaagagat tagttccttt ctctagagaa
     2161 ttgtatatag aacaagatga ttttatggaa gaacctgtta agaaatattt tagatttttc
     2221 ccaggaaatg aagtccgttt aaaaggtgct tattttgtaa aatgtactga tgtaattaaa
     2281 gatgaaaatg gaaatgtagt tgaaatacat ggtacttatg accctgaaac taagagtggc
     2341 tctggcttta caggacgtaa ggtaaaatct acaatacatt gggttgatgc taaatcagct
     2401 ataccatgtg aatttagatt atttgaacca ttaattcttg atgatatacc tgaaaatgaa
     2461 ggtaaacatt tcttagaaca aataaatcct aattcattgg aaatattgca aggttttgtt
     2521 gaacctacac agataaaaga tgctaaacct tttgataaat tccaatttgt cagaaatgga
     2581 ttctttagta tagataataa atatacaact gatgaaaaat ttgtatttaa tagaatagtt
     2641 cctctaaaaa gttcttttaa accaggtaaa taatcttaca atcaaaaaat gaagacttaa
     2701 aatataaatt acttctacta taaataaact tagttagaag taatttttta tttgagtctt
     2761 cattatttac tttaaatgat taactttttt cactacagat tcccataact tctattccat
     2821 cttttatgca ttctatgtta agaggaaaat ctggtccttt ttctccgtca atatctgtta
     2881 taagtgaatc ttcacattct atatgcacct ttttagtttt aaagtatagt atttcatttc
     2941 cattatattg atctaaataa tcacctctaa gcacacttat caaaactggt attgattttg
     3001 gtattggcat tcctttaaaa ataattacat ccaaatatcc atcatctatt tcagccttat
     3061 atgctagatt tatatttcct gctgttttgc cattaaaaat caacataaga tacatatctc
     3121 catcataata catttcctca gaagatactt ttatcttaaa tttcctcata tacaatgctt
     3181 cttctattcc ctttatataa taagaaattc ttccaaatga attttttaag tctgtgttta
     3241 ttctttgtga tacatccgta aacataccag cacttgcaac atttataaag tacttattat
     3301 tgactttccc tatgtctatt ttctttggtt ttgaatttat gatttcattt atagcttctt
     3361 ttatatcaaa tgaaatttga agagcatttg aaaaatcatt tgcagttcct gtaggcaaaa
     3421 ttccaattgg aatttttatg tctaattctt tcattgcatt tactactaaa tctacagtac
     3481 catcaccacc agctatcaat atgtgttcat aagaattatc aacttcctta aatgcattct
     3541 ttacaggttc tttaatagtt aatctatagg gtatcaataa ataatttttt tcttgatata
     3601 attctatgat agtgtctaaa tttgataata tctttttttc acctgaatta ggattgtata
     3661 taagttttac ttttttcatt atttctcctt actcatttta ttcttttagt atccaaaatc
     3721 ttcatttaaa aaaatcacat gtattctttc tggtgttaat tgtcttttta taagtgtata
     3781 actacagcat attcttcctg gacttgaaca gtctatacat ataccagtag tcttacatcc
     3841 agtatttgta cttaatcttt tacaattggc tggagcagct attgtttttg ttctttttat
     3901 agcttcatct acatctttaa ctattttatt tactcctgct ataactatta ctttatcagg
     3961 tccatatatc atagcagcaa ctctattccc attcccatct aaattgtaaa gttctccatc
     4021 ctctgttatc gcattagtac ttgtaaaata agcatctgca gaaaatgact ttctgtatat
     4081 ttccttcata tcttctggtt tgataccttt tgcatatcta tcaagaaaat tatatttttc
     4141 tcttataaaa tcaatcactc ctgtttcaaa taaggtcata gaacctccac atcctactag
     4201 agaacccact ttaactattt cttcaatttt tttaagaagc tcttctttgt tacttactaa
     4261 gtaccctttc atattattgt tttccagaga ttttattgtt ttttctattc ttttttcatt
     4321 aagccaattt aaattttgat ccattttaac ttccccctta atttttaata tgtacttaat
     4381 tatttataaa ctttattttc tttataagtt tattttagta catctaaaaa aaaatacaat
     4441 ataaaactta aatagttttc cataattttt tccaaaataa cttactcaat caaaaaagga
     4501 aagtagatgt actattctac tttccctata ttgtatgtag tattatatat tgcctaaaat
     4561 ctatatatta attatatgtt tcatattata gaatatcaca gttaattaaa atgtaatact
     4621 atattctttt taaacatata aattgtatta ttaaatttaa tatatccaac ttcaataaat
     4681 cttattaatt tgatgaagaa gatgctatag ctgcacttgt tgctgccatt acacatgcaa
     4741 tttgaatagc aacaacaata tttaaaaata tattattagt tatagaacct acattattct
     4801 catttcgtat caaatctgca taaattgaag ctacaatagc agaactaatc attactctat
     4861 tactttttcc taaagaccaa tttccaaagc ctttttcttc tttaagttta tttgaaacat
     4921 tttttatctc ttctattatt tcatcttctc tataatctaa taatgaaatt gctcctatta
     4981 ttggatatcc ataataattc attttacaat cattttcttt aaatgctttt ttaagttcaa
     5041 tagtcttctt acatttaagc atagttctat catcactaaa taaaagaatt tgggataatg
     5101 cttgtaaatc atttccttta taaaaaccat tttcatttag aaattcataa catttttcta
     5161 tgtattctaa atcttcatct aaattttttg aatgaattgc aataagtgct atattacaat
     5221 agtcatcact agaagttata aatggatgtt ttttcctcat gtaatcatat attattttca
     5281 tttttgatat acatgtaagg taatccattt tctctctatt ttcataaact attgatgctg
     5341 taagtggtaa atatgtatcg ccccaaaact tttcactttt aagttctttg tatatatcta
     5401 aaatcatctt aaaactactt tctggatttg attcaaatga aagtagtgtt gctatataaa
     5461 atatattata tcctctaaaa ttagagaata atccagtatt ttttttaata agctctattc
     5521 gttcttctac taatctagga cttattctct tgttcttaag ggtaaatgca agtgcacaac
     5581 tatgttgtat caatcccatt tgccaagaac ctttaacttc atttaatgct ttgtaatttt
     5641 ctattaataa gtttactttt ccatttataa aagattgttc catattgccc tcctagtaat
     5701 atatctcatt gtattaatag ttttatattt attatatata tattaaatac tacaatcaat
     5761 acatattact tcaaaggaca tcattcttca acaataatgt agcctttttt gtaatgtctt
     5821 cccatattat tgactctatc atagtactct gattcacttt cattactcca tttgggcaca
     5881 aatacatcta ttgcattaga attactaaaa tacttactag accccctatc cataacagtt
     5941 tttaattttc catctacaat aatcttagtt ccaaaactca agtgattgtt tgctacaaaa
     6001 ccatcttcta aatcatctcc tgtagcagtt atcgctccat atccattttc ttgattaaga
     6061 cttgtgtaat aactaacttc aaaagttata ggtactaact tatttatttt aatacccaac
     6121 tgattttgta gtttctcctt ttgtaccttc aacttatact catcactatt tttatagtct
     6181 tccatacctt gattatactt ggaattaaag cttttacctg actttactat agtatctata
     6241 tttactatag attctttaca atgatctgca tctatttttg tttctccatt taccaataat
     6301 ggagttaaac atacactaca tacaacacat gtaatcaatg cttctttaag cttcataaat
     6361 tcccccctta ttattttgat atattagcat atttaatatt atatgtctac atttagattt
     6421 aaataccaca cccatttgct ttaagaagtt gtcttatatt taattcacct taaacactta
     6481 taaatcctct atcttagaat catatagaat aaagctttta tcgttaaata ttttttatta
     6541 atcttttaaa atctaaaact actgtattta attttctctt ttttagaatt taagaattaa
     6601 aattctataa ttttaaaaca tattgaattt atttttttga gatgatataa ttttcttaag
     6661 aggtggttga tatgataaaa aatacaaaac aaattacagt agaagttaat gaaattttat
     6721 tacaagaaat atctaagata actaatgacg aaaataatat tttgacagat ttgataaatg
     6781 aatcaattgt aaattatatt cttgaaaaca aaaaacataa cattgaaaat aaagatttag
     6841 aagaagtgat taaattttac aaaaaagaaa atactgaaaa acctaaaagt gcagcaagag
     6901 ctatagctga agcaaaaact cataccctgt attaatcaaa taaaaaagtt cctaaaaact
     6961 aggaactttt ttattttaat aattattaat atatctcaat atttatctca aatttatcaa
     7021 tataagcatg tttaatacaa tctattgcat acttatctta ccctagaaat aatcaaaaca
     7081 aaaataaaat tatgcccttt atatctaaaa caattaattt acatgattaa tatacaaaaa
     7141 ttctgacttt taaattcgta tacaatatca aactaaaata ctattaataa caaacccaat
     7201 ataaattaaa ttcaaacagg tttgatttta catcaaactt attgtattaa tattaaataa
     7261 caagacaaaa atatttataa aaatcacaaa ttttacataa aactttatat ttgattcatt
     7321 taactaatta ttgtaaaaac tatttatttt atgataatta actacaccaa attattcatc
     7381 ttttttaata tctttaaccc atttttcaac agagttcaat tcttcttcat ttaccccctt
     7441 tttgaacaaa gatttcataa aactctcaat agaacttcca tgcatattat ctaaaaaagt
     7501 ttttgtttca aaatgtaaat actcttcttc tccaataaga actgtataat gtgtatgtct
     7561 atcaatcttt cttgcactta agaattgctt atttactaat cttgataaaa gtgttagtgt
     7621 tgtagtttgc ttccaattat atagtttttc catagcttca atgacatctt ttgatgctac
     7681 tgtaaaatct gaactccaaa taaatttcat gacttttaat tctgctggtg gtatcttagt
     7741 aatagtcata tgattttaca ctcctctcaa acttctacat aattaaatac tatattaata
     7801 gacttttcga gtcaaaggtt ttatttaaaa tatctcatcc aaatttatta atacaaaaaa
     7861 tttatatttt tattcaaaat taaacacaca atctattaat ataaagataa atatctatta
     7921 tataagtata acagattatc aaattaaaat tctaat
//
LOCUS       DAEPXF010000170         7927 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain HB27
            SAMN13376389-rid12479423.denovo.177, whole genome shotgun sequence.
ACCESSION   DAEPXF010000170 DAEPXF010000000
VERSION     DAEPXF010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13376389
            Sequence Read Archive: SRR10518432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7927)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7927)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001069624.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001069624.1
            Genome Coverage       :: 315x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/15/2021 01:54:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,165
            CDSs (total)                      :: 4,076
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 89
            rRNAs                             :: 10, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 10 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 19 of 69
            Pseudo Genes (incomplete)         :: 44 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 10 of 69
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7927
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13376389-rid12479423.denovo.177"
                     /strain="HB27"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="China: Hebei"
                     /collection_date="2017"
     gene            1..75
                     /locus_tag="KRL67_003138"
     tRNA            1..75
                     /locus_tag="KRL67_003138"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:33..35,aa:Thr,seq:tgt)
     gene            90..174
                     /locus_tag="KRL67_003139"
     tRNA            90..174
                     /locus_tag="KRL67_003139"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:124..126,aa:Tyr,seq:gta)
     gene            184..267
                     /locus_tag="KRL67_003140"
     tRNA            184..267
                     /locus_tag="KRL67_003140"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:218..220,aa:Leu,seq:tag)
     gene            292..366
                     /locus_tag="KRL67_003141"
     tRNA            292..366
                     /locus_tag="KRL67_003141"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:324..326,aa:Gly,seq:gcc)
     gene            391..467
                     /locus_tag="KRL67_003142"
     tRNA            391..467
                     /locus_tag="KRL67_003142"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:425..427,aa:Arg,seq:tct)
     gene            477..552
                     /locus_tag="KRL67_003143"
     tRNA            477..552
                     /locus_tag="KRL67_003143"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:510..512,aa:Gln,seq:ttg)
     gene            561..636
                     /locus_tag="KRL67_003144"
     tRNA            561..636
                     /locus_tag="KRL67_003144"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:594..596,aa:Lys,seq:ttt)
     gene            639..727
                     /locus_tag="KRL67_003145"
     tRNA            639..727
                     /locus_tag="KRL67_003145"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:673..675,aa:Ser,seq:tga)
     gene            731..806
                     /locus_tag="KRL67_003146"
     tRNA            731..806
                     /locus_tag="KRL67_003146"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:764..766,aa:Phe,seq:gaa)
     gene            813..886
                     /locus_tag="KRL67_003147"
     tRNA            813..886
                     /locus_tag="KRL67_003147"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:847..849,aa:Met,seq:cat)
     gene            901..977
                     /locus_tag="KRL67_003148"
     tRNA            901..977
                     /locus_tag="KRL67_003148"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:935..937,aa:Ile,seq:cat)
     gene            995..1071
                     /locus_tag="KRL67_003149"
     tRNA            995..1071
                     /locus_tag="KRL67_003149"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1029..1031,aa:His,seq:gtg)
     gene            1080..1155
                     /locus_tag="KRL67_003150"
     tRNA            1080..1155
                     /locus_tag="KRL67_003150"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1113..1115,aa:Lys,seq:ttt)
     gene            1163..1236
                     /locus_tag="KRL67_003151"
     tRNA            1163..1236
                     /locus_tag="KRL67_003151"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1195..1197,aa:Cys,seq:gca)
     gene            1244..1320
                     /locus_tag="KRL67_003152"
     tRNA            1244..1320
                     /locus_tag="KRL67_003152"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1278..1280,aa:Arg,seq:acg)
     gene            1333..1408
                     /locus_tag="KRL67_003153"
     tRNA            1333..1408
                     /locus_tag="KRL67_003153"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1366..1368,aa:Val,seq:tac)
     gene            1484..1897
                     /locus_tag="KRL67_003154"
     CDS             1484..1897
                     /locus_tag="KRL67_003154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH1524001.1"
                     /translation="MYIVIGNEIVDSEELKIIIDKNSKFKVEKDLSKSTKREDVIAYQ
                     LSIDLNYLDSLINEQCNLASLSDEEKFDEYMTLSDELALDLEELMPKYTIINARAYKL
                     DEVDGIVKIILVVAYADLGHLKLSDVVKRLSRQVD"
     gene            2206..5637
                     /locus_tag="KRL67_003155"
     CDS             2206..5637
                     /locus_tag="KRL67_003155"
                     /EC_number="6.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate carboxylase"
                     /protein_id="HBH1524002.1"
                     /translation="MLKKFNKILVANRGEIAIRIFRACSELGIKSVGIYSKEDKYGLF
                     RTKADESYLIGEGKGPIDAYLDMDGIIDLAKRKKVDAIHPGYGFLAENAEFARKCEEN
                     GITFIGPSSKVMNMMGDKINSKKIAKEVNVQTIPGVEKAIRSTEEAKEVANKIGYPVM
                     IKASNGGGGRGMRIVHREEDLELEYETACSESRKAFGEDIIFIEKYIADPKHIEVQIL
                     GDNYGNIVHLYERDCSVQRRHQKIIEYAPAFSLDDKVRKEICEDAVKLSKHVGYSNAG
                     TLEFLVDANGGHYFIEMNTRVQVEHTVTEMVTGIDIVQSQILIAQGYSLDSEEINIKS
                     QDDVEIRGYSIQCRITTEDPKNKFMPDTGKIQVYRTGSGFGIRLDGGNGFTGANISPH
                     YDSLLVKTISWDRTFQGAINKTIRSIKELRVRGVKTNVGFLVNVLNNPIFSNGKCSTK
                     FIDENPDLFEITESKDRGTKLLQFIGDVIVNDNACKEKPLFDALHDPRMDKDGSKSEG
                     SKILFDKLGKSAYIEKIKNDKKLLLTDTTMRDAHQSLLATRIRTYDLLKAAKPTEKYQ
                     KDLFSLEMWGGATYDVAYRFLKESPWRRLQKLREEIPSIMFQMLLRASNGVGYKNYPD
                     NVIEEFTKESARQGIDVFRIFDSLNWVENMKPSINTALETGKIVEATMCYTGDILDKT
                     KTKYNLEYYIKMAQELESLGADIIAIKDMSGLLKPYSAYTLVKELKKNVKAPIHLHTH
                     DTSGNGVATCLMASEAGVDIIDAALESMAGLTSQPSLNAIVEALKNTERDTGIDLFGY
                     DELGKYYKDLRKVYNKFESDLTNSCAEIYNFEIPGGQYTNLKPQADSLGLVNRFDEVK
                     EKYKEANEVVGDIIKVTPSSKVVGDLAIFMTKNKLDKDNIIEEGKNLSFPDSVVDYCK
                     GMIGQPEGGIPKDLQEVVLKGEEAITVRPGSLLPAEDFDEIAKYLNEKYDINANIRNV
                     ISYALYPKVYEDYIKHLQHYNDISKLESDVFFYGLNKNEECEVEIEEGKVLTIRLVEI
                     GEVKENGFRTIGFELNGMVREVEIKDKNFSGKINNVEKADMNDPLQIGASIPGKVIKI
                     MVKEEDEVKANQPLIVIEAMKMETIIVAKTDGVIKSIKVKEDDMVEDKQLLMIMK"
     gene            5879..7807
                     /locus_tag="KRL67_003156"
     CDS             5879..7807
                     /locus_tag="KRL67_003156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HBH1524003.1"
                     /translation="MKVYDSKMLRNVAVLGHSGCGKTNLIETIAYTANTNKIPKLTDK
                     VNMTYSMGLIPIEYNDYKFNLLDTPGYFDFSGDVVSSLRASDAAIIVIDATAPIQVGT
                     EKSLELTESIPKIMFINKIDNEKARYKDAIAMLREKYNNKIVPMISPIYKDKNFVKLH
                     NVFENIDDLEGEFKEQAMSVKEALMELIAETDDQILDKYFNGEELTTEEIQKGIIIGI
                     QRGDIIPVICGSTINNIGTKEILDTISSYLEPIFTEESKPFRGLVFKTMVDPFVGKMS
                     YIKITEGVLSKDKDVFNINKNVKEKIANIYTLRNSELVEIEKAKAGDIVVITKVNSLK
                     TGDTISADKDAEALEKIDFPKPQIYYAVTPKNKGDEEKVASVLNKLVEEDPTLHWYRN
                     TETKQALLGGQGELHIKTIKNKMKDKFGVDVELNDLKVPYRETIKGTADVQGKHKKQS
                     GGHGQYGDVKIRFERCESDFEFTEEIFGGSVPKQYIPAVEKGLKDSMQKGILAGYPVT
                     NIKATLYDGSYHDVDSSEMAFKMAASAAFKKGMEEAHPILLEPIMKLKITVPEEYMGD
                     VMGDINKRRGKIFGMEPDDKGKQIIFAEAPQAETFKYAIDLRAMTQGRGYFEMELERY
                     GEVPSQFAEKIIGLATAK"
BASE COUNT         3102 a         1013 c         1591 g         2221 t
ORIGIN      
        1 gctggtgtgg ctcaacggta gagcagctga cttgtaatca gcaggttgta ggttcgattc
       61 ctatcaccag ctccatgtaa aattaatacg gaggatttcc cgagcggcca aagggggcag
      121 actgtaaatc tgttgtcatc gacttcggtg gttcgaatcc accatcctcc accaaaaaaa
      181 tgcgcggatg tggcggaatt ggcagacgca ctagacttag gatctagcgc cattggcgtg
      241 ggggttcgac tcccttcatc cgcaccattt ttatttaatt ttaataaacg agcgggaata
      301 gttcagtggt agagcgcaac cttgccaagg ttgaagtcgc gagttcgaat ctcgtttccc
      361 gctccaaatt ttgtgtttat attgtaatgt gggtgcatag ctcagctgga tagagcaacg
      421 cccttctaag gcgtgtgtcc ggggttcgaa tccctgtgcg ctcaccacat atatattggg
      481 gattagccaa gtcggtaagg cacatgactt tgactcatgt atgcgtaggt tcgagtcctg
      541 catccccagc caaatagtat ggtctattag ctcagtcggt agagcacctg acttttaatc
      601 agggtgtccc gcgttcgagt cgcggataga tcaccaatgg agaggtgtcc gagtggttta
      661 aggagctggt cttgaaaacc agtgacttcg aaaggggccg tgggttcgaa tcccaccctc
      721 tccgccaaat gcccagatag ctcagtcggt agagcagggg actgaaaatc cccgtgtcgg
      781 tggttcgatt ccgcctctgg gcaccagaat atggcggtat agcttagttg gctagagcgt
      841 tcggttcata cccgaaaggt cataggttcg actcctatta ccgctactaa aatttaatgt
      901 gggccattag ctcagttggt tagagcgccc ggctcataac cggtaggtct ggggttcgag
      961 tccctgatgg cccaccacat taaattaact tatggtgggt atagctcagt tggttagagc
     1021 gccagattgt ggctctggag gtcgtgagtt cgactctcat tatccacccc aaattatatg
     1081 gtctattagc tcagtcggta gagcacctga cttttaatca gggtgtcccg cgttcgagtc
     1141 gcggatagat caccaaaaag gcggcgacat agccaagtgg taaggcagtg gactgcaact
     1201 ccttgatccc cagttcgaat ctgggtgtcg cctccaataa aatgcgctca tagctcaact
     1261 ggatagagtg tctgactacg aatcagaagg ttaggggttc gagtcccttt gggcgcacca
     1321 tataaatagc gagggattat agctcagctg ggagagcacc tgccttacaa gcagggggtc
     1381 acaggttcga gccctgttaa tcccaccagt gcaaacctct agtaaaacta ctagaggttt
     1441 tatttttgat tgaaaaagtt tttatattta ggaggtaaga aacttgtata tagtaatagg
     1501 aaatgaaata gttgatagtg aagaacttaa aataattata gataaaaata gtaaatttaa
     1561 agttgaaaaa gatttaagta aaagcacaaa aagagaagat gtaatagcat atcagttaag
     1621 tatagattta aattacttag attctcttat aaatgagcaa tgtaatttag ccagtctatc
     1681 tgatgaagag aaatttgatg aatatatgac tttatctgat gaacttgcac tggatttaga
     1741 agagctaatg ccaaaatata caataataaa tgctagagca tataaattag acgaggtaga
     1801 tggaattgta aaaataatac tagttgtagc gtatgcagat ttagggcact taaaactttc
     1861 tgatgtagta aagaggctat ctagacaagt agattaggat atgaatatga actatattaa
     1921 aaaaatgtat aaaatcttac gtaaagatga aaataattat gtaaacatag aaaaaaacag
     1981 tataaaatca agtcaattgt aggaattttc acataaattc atctgaaatt ctataaaaaa
     2041 tgccttaatt ttaatgaggt aaatcgagtt tacacagtct aaaaattact tatatttata
     2101 aatttatctt attaattaag gaactttctt aaacatcttc aaaaataaag tattttagtc
     2161 tataatcttt aatattacac aaacaataaa ggagaagaat agagtatgct taaaaagttt
     2221 aataaaatac tagtggctaa tagaggcgaa atagctataa gaatatttag agcctgttca
     2281 gaattgggga taaaaagtgt tggtatatac agtaaggaag ataaatatgg attgtttaga
     2341 acaaaagcag atgaatcata tttgatagga gaaggtaaag ggccaataga tgcatattta
     2401 gatatggatg gaataataga cctagcaaag agaaaaaaag ttgatgctat acatccagga
     2461 tatggttttc tagcggagaa tgcagaattt gctcgtaaat gtgaagagaa tggaattact
     2521 tttataggac catcatctaa ggtaatgaat atgatggggg ataagattaa ctctaaaaaa
     2581 atcgcaaaag aagttaatgt tcaaactata ccaggagtag aaaaagcaat aagaagtact
     2641 gaagaagcaa aggaagttgc aaacaaaata ggctatcctg ttatgataaa agcatctaat
     2701 ggtggtggcg gtagaggtat gagaattgta catcgcgaag aagacttaga gcttgaatat
     2761 gaaactgctt gtagtgaatc aagaaaagct tttggagaag atattatatt tatagaaaaa
     2821 tatatagctg atccaaaaca tatagaagtt caaatattag gagataacta tggaaatata
     2881 gttcacttat atgaaagaga ttgttctgtt caaagaagac atcaaaaaat aatagaatat
     2941 gcaccagctt tttcgttaga tgataaagta agaaaagaga tatgtgaaga tgcagttaag
     3001 ttatcaaaac atgtgggtta ttctaatgca ggaacattag aatttttagt agatgcaaat
     3061 ggtgggcatt attttataga gatgaacaca agagtacagg ttgaacatac tgtaacagag
     3121 atggttacag gaatagatat agtgcaaagt caaatactaa tagcccaagg ttacagttta
     3181 gatagtgaag aaattaatat aaaatcacaa gatgatgtgg aaataagagg ttactctatc
     3241 caatgtagaa taactacaga agaccctaag aataaattca tgcctgatac aggtaaaatt
     3301 caagtttata gaactggttc aggatttggt ataagactag acggaggaaa tggatttaca
     3361 ggggcaaaca taagtcctca ctatgatagt ttattggtta agacaatatc ttgggataga
     3421 acattccaag gagcaataaa taaaactata agatctataa aagaacttag agttagaggt
     3481 gttaaaacta atgtaggatt cctagttaat gttttaaata atcctatatt ctcaaatggt
     3541 aaatgtagta ctaagtttat agatgaaaat ccagatttat ttgaaataac agaaagtaaa
     3601 gacagaggaa caaaattact tcaatttata ggagatgtaa ttgtaaatga taatgcttgt
     3661 aaagaaaaac cattatttga tgcattacat gacccaagaa tggataagga tggctcaaaa
     3721 tcagaaggaa gtaaaatact tttcgataag ttaggtaaat ctgcatatat agaaaagata
     3781 aaaaatgata agaaattatt attaacagat acaacaatga gagatgcaca tcaatcatta
     3841 ttagcaacaa gaataagaac atatgactta ttaaaagctg ctaagccaac agaaaaatat
     3901 caaaaggatt tattctcact tgaaatgtgg ggaggagcta cttatgatgt tgcatataga
     3961 ttcttaaaag aatctccttg gcgtagatta caaaagctaa gagaagaaat accaagcata
     4021 atgttccaaa tgttgcttag agcatcaaat ggagttggat ataaaaacta tccagataat
     4081 gtaatagaag aatttacaaa agaatctgct agacaaggta tagatgtatt tagaatattt
     4141 gattcattaa actgggttga aaatatgaag ccatctatta atacagcttt agaaactgga
     4201 aaaattgttg aagcaactat gtgttataca ggtgatatac tagataagac taaaactaaa
     4261 tacaatctag aatattatat taaaatggca caagaattag aaagcttggg agcagatata
     4321 atagcaataa aagatatgtc aggtcttctt aaaccatatt cagcttacac actagttaaa
     4381 gagttaaaga aaaatgtaaa agcaccaata catttacaca ctcatgatac aagtggaaat
     4441 ggtgtagcaa cttgtttaat ggcatcagaa gctggagtag acataattga tgctgcatta
     4501 gaatctatgg caggacttac aagtcagcca tcacttaatg ctatagtaga ggcacttaaa
     4561 aatacagaga gagatacagg tatagattta tttggatatg atgagttagg aaaatattat
     4621 aaggatttaa gaaaagttta taataaattt gaaagtgatt taactaattc ttgtgcagaa
     4681 atatataatt ttgaaatacc aggaggccaa tatacaaact taaaacctca agctgatagt
     4741 ttaggattag ttaatagatt tgatgaagtt aaagaaaaat ataaagaagc taatgaagtt
     4801 gtaggagata taataaaagt aactccatct tcaaaagttg taggagattt agctatcttt
     4861 atgactaaaa ataaattaga taaagacaat ataatagaag aaggtaaaaa tttatcattc
     4921 ccagattctg ttgtggatta ctgtaaaggt atgattggtc aaccagaagg tggaatacca
     4981 aaagatttac aagaggtagt tctaaaaggt gaagaagcta taacagtaag accaggttca
     5041 ttattaccag cagaagattt tgatgaaata gctaaatatc taaatgaaaa atatgatata
     5101 aatgctaata taagaaatgt tataagttat gctttatatc caaaagtata tgaagattat
     5161 atcaaacatt tacaacatta caatgatata tctaaacttg aaagtgatgt attcttctat
     5221 ggattaaata aaaatgaaga atgtgaagtg gaaatagaag aaggtaaggt attaacaata
     5281 agattagttg aaatcggtga agttaaggaa aatggattta gaactatagg atttgagtta
     5341 aatggaatgg ttagagaagt agaaataaaa gataagaatt tctctggaaa aattaataat
     5401 gttgaaaaag ctgatatgaa cgaccctctt caaattggag caagcatacc aggaaaagtt
     5461 ataaaaataa tggttaaaga agaagatgaa gtaaaagcga atcaaccatt aatcgtcata
     5521 gaggctatga agatggaaac tataatagtt gctaagactg atggagtaat taagtcaatc
     5581 aaagtaaaag aagatgatat ggttgaagat aaacaattat taatgataat gaagtaattg
     5641 aattaaaata aattaaaaag cactctgaaa tgtttaataa aaaatgtttt agagtgtttt
     5701 tatttatttt aattaaatat ttgttaaaaa aaagaggaca atcacctcca gatgtagaaa
     5761 taaagctagt aaggaaaatg ttatctaaaa attctaaata tagtaaatat tttccataat
     5821 atttcttagg aaataacagc aaagataaaa tacataaatt taaaaggtgg gtgcttatat
     5881 gaaggtttat gatagtaaaa tgttgagaaa tgtagcggta ttaggacata gcggatgtgg
     5941 aaaaacaaat ctaatcgaaa ctatagctta cacagcaaac actaataaga tacctaaatt
     6001 aacagacaag gtaaatatga cttatagcat gggattaata cctatagagt ataatgatta
     6061 taagtttaac ttattggata ccccaggata ttttgacttt agcggagacg ttgtgtcatc
     6121 tcttagagca agtgatgcag caataatagt aatagatgca acagcgccta ttcaagttgg
     6181 tacagagaaa tcattagaac taactgagag tattcctaag ataatgttta taaataaaat
     6241 agataatgaa aaggcaagat acaaagatgc aatagcaatg ttaagagaaa aatacaataa
     6301 taagatagtt cctatgataa gtcctatata caaagataaa aactttgtaa agttacataa
     6361 tgtttttgaa aacatagatg atttagaagg tgaatttaag gaacaagcta tgagtgttaa
     6421 agaagcatta atggaactta tagcagaaac tgatgaccaa atattagata aatattttaa
     6481 tggagaagaa ctaactacgg aagagataca aaaaggtatt attataggta tacaaagagg
     6541 tgatattata cctgtaatat gtggttcaac tataaataat ataggcacaa aagaaatatt
     6601 agatacaata tcaagttatt tagaacctat ctttacagag gaaagcaaac catttagggg
     6661 attagtattt aaaactatgg ttgacccatt tgttggaaag atgtcatata taaaaattac
     6721 tgagggtgta ctttctaagg ataaagatgt ttttaatata aataaaaatg taaaagaaaa
     6781 aatagctaat atatatacat tgagaaatag tgaattagta gaaatagaaa aagctaaagc
     6841 tggtgatata gtagttataa ctaaagttaa ttctctaaaa acaggagaca ctatatctgc
     6901 agataaagat gcagaagctt tagaaaagat agattttcct aaaccacaaa tatattatgc
     6961 agtaactcct aagaataaag gtgatgaaga aaaagtagca tctgtattaa ataaactagt
     7021 agaagaggac cctacattac attggtatag aaatacagag acaaaacaag cacttttagg
     7081 aggtcaagga gaacttcata taaaaacaat taaaaataaa atgaaagata agtttggtgt
     7141 tgatgttgaa ttgaatgacc ttaaagttcc ttatagagaa actataaaag gaacagctga
     7201 tgtacaagga aaacataaaa agcaatcagg tggtcatggt caatatggag acgtaaaaat
     7261 aagatttgaa agatgtgaat ctgattttga atttacagaa gaaatatttg gtggttctgt
     7321 accaaaacaa tatataccag cagttgaaaa aggtctaaaa gactctatgc aaaaaggtat
     7381 attagcaggt tatcctgtta caaacataaa agctacgctt tatgatggtt cataccatga
     7441 tgtagactct tctgaaatgg cctttaagat ggcagcaagt gcagccttta aaaaaggcat
     7501 ggaagaagcg cacccaatac tactagaacc tataatgaaa cttaagataa ctgttccaga
     7561 agaatatatg ggagatgtta tgggtgatat aaataagaga agaggaaaaa tatttggaat
     7621 ggaaccagat gacaaaggga aacaaataat atttgcagaa gctccacagg cagagacatt
     7681 taaatatgca atagatttaa gagcaatgac acaaggtaga ggatactttg aaatggaatt
     7741 agagagatat ggagaggttc catcacagtt tgcagaaaaa ataattgggt tagctactgc
     7801 aaagtaatga acaaaataaa tagaatcata aataattaat agatactatt aaaaatattt
     7861 ttagggtttt aactaggtat aagtttatgt tagataaact tatacctagt ttttatatat
     7921 ttttgat
//
LOCUS       DAEPXF010000171         7796 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain HB27
            SAMN13376389-rid12479423.denovo.178, whole genome shotgun sequence.
ACCESSION   DAEPXF010000171 DAEPXF010000000
VERSION     DAEPXF010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13376389
            Sequence Read Archive: SRR10518432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7796)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7796)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001069624.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001069624.1
            Genome Coverage       :: 315x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/15/2021 01:54:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,165
            CDSs (total)                      :: 4,076
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 89
            rRNAs                             :: 10, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 10 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 19 of 69
            Pseudo Genes (incomplete)         :: 44 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 10 of 69
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7796
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13376389-rid12479423.denovo.178"
                     /strain="HB27"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="China: Hebei"
                     /collection_date="2017"
     gene            116..1519
                     /locus_tag="KRL67_003157"
     CDS             116..1519
                     /locus_tag="KRL67_003157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBH1524004.1"
                     /translation="MEKKKIGLWSLVFMNVSALYGIRWIAKSTASSFGLGLGAIPMWL
                     LFSFIYFVPGALICAELASTYQKGDGGLYDWVKEAFGEKYGFLVSWLNWTAKIFWYSS
                     FLTFLAVNVSYTIGNPALADNKMFVLILSLIIFWALSLIATKGISFTKIFTNTGALGS
                     TIPSAILIIFSFVAVFVLKKHDIASTYTIASMTPKLNMDAFVAISAIMFGLAGAETAA
                     NFITEIDKPEKNFPKAILISAGIVASLYVLGSIAITMIIPPDQITASKGVLDALSAVC
                     ASLGIGSWLVQLIAFGIAFSVLGAIVLYIASPIKMLFGSVKKGIFPDSLVEVNEHKIP
                     SKAVILQAIIVTIILLVTTLMPSVDAIYNVLVTMTALTALFPYVLLYASYIKLRKERP
                     DEIRPYTMAKSTSTCLGLAKMVLVVTVVGILLSAAPVMPTFAENVIYEIEMIGGGLLV
                     ILSGLWLWNRYEKKTAK"
     misc_binding    1610..1850
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            1893..2591
                     /locus_tag="KRL67_003158"
     CDS             1893..2591
                     /locus_tag="KRL67_003158"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HBH1524005.1"
                     /translation="MNVIGIIGAMDEEVNILVDLMDIRETIKKASLEFYKGILEGKNV
                     VLVKCGIGKVNSALCAQILISEFKVDAIVNTGVAGALNEKLDVNDIVISTDAIQYDVD
                     TTAFGDPKGVIPRMKTSVFKADERLIDAAYKSSVEEVKTHKVLKGRVVTGDKFINSKE
                     LKEELVNDFGGYCGEMEGGAIAHVCYLNNTPFVIIRAMSDKADGSADVTYDVFVHDAA
                     NNSKDIVLNMLKSI"
     gene            2614..3615
                     /gene="trpS"
                     /locus_tag="KRL67_003159"
     CDS             2614..3615
                     /gene="trpS"
                     /locus_tag="KRL67_003159"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HBH1524006.1"
                     /translation="MSEKKVIFSGAQPSGKMTLGNYLGAIKNWTELQDNYDCYYSIVD
                     LHAITVPQDPKVLRANAIELLAQYIACGLDPEKNTIFIQSHVKEHVELMWVLNTMTYM
                     GELSRMTQFKDKSQKSEANLNAGLFTYPVLMAADILLYQTDLVPVGDDQKQHLELARD
                     LANRFNNRFSPTFVVPEGYYPKGGARVMSLQEPTKKMSKSDSNENAFILLADDSDSIK
                     RKIKRSVTDSLGVVKYNDEQPGIKNLIDIYSNLSKKSVEDIVNMYEGKGYGIFKEDVA
                     EVVSEALRPIREKYVDLLNNKDYLEKIYSMGAEKAEKQARKTLRKVYKKVGFIERRY"
     gene            4202..5404
                     /locus_tag="KRL67_003160"
     CDS             4202..5404
                     /locus_tag="KRL67_003160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HBH1524007.1"
                     /translation="MKTSILIALIEKTSLIVVLFLLITKLKIFKQIFQKEEYSFNDLV
                     CIALVFTFLAIFGTYSGINYMGSIVNTRIISIVSGGILFGPMVGITAGVFSGIHRYFM
                     DIGGITSVPCLLSSILAGVLSGFLYKRIPKQHRVMYGILVGMISESFTILLIYLISYP
                     HSLAIQIIGGIYLPLIVGQIGIGFVISIVEGIEKDKKDIEARNKAEIKALQRQINPHF
                     LFNSLNTIASFIRFNPDKARELIINLSTYLRYNLEYSDNLIDINKEIEQVKSFVEIEK
                     ARFGELLTVSYDIEDVNIKIPSLIIQPLVENAIIHGILESGRAGVVKISIKKLPYSLE
                     NTVRISIEDNGIGISEEIINNVYQDNMPENKIGLYNVHLRLKLMYGRGLNIRRIDTGT
                     LIVFYVKE"
     gene            5409..6179
                     /locus_tag="KRL67_003161"
     CDS             5409..6179
                     /locus_tag="KRL67_003161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBH1524008.1"
                     /translation="MRAIIVEDEFPARKELRYFIENKSGIEVVSEFTNGIEVLDFIQE
                     NKIDVIFLDINIPHLDGMLLAKTLNQFKSRPKIVFITAYENYAVDAFSLDVFDYILKP
                     YSEERIISMLNKLEKSEMSDIELSNGNSNLYKYKKEAVNQEIEEITHKISLWKGDKLV
                     VIDIDDIYYCEANERQTFIYTEKEKFILKEGISEVENLINDKTFFRTHRSYIVNLTKV
                     KEIIPWFNNTYILKLKNSDYEVTVSRSKVKDFRLLMHI"
     gene            6325..7746
                     /locus_tag="KRL67_003162"
     CDS             6325..7746
                     /locus_tag="KRL67_003162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation protein A"
                     /protein_id="HBH1524009.1"
                     /translation="MVSFLGSIVVLIVGYFVYGTFVEKVFGINDKNQTPAIACRDGVD
                     YVPMKWKRIFLIQFLNIAGLGPIFGAIQGALFGPSAFLWIVFGTIFAGGVHDFASGYL
                     SMKNKGTSASELVGLYLGNGAKIAMRIFSVILLVLVGVVFVTGPAGLLKTLTGVNAQI
                     WVGVIILYYIMATVLPIDKLIGKIYPLFGAALLIMAVGVAGGLIIKGYNIPNINLQNM
                     NPNGTPLFPYLFITIACGAISGFHATQSPLMARCVEKESEARPVFYGSMVAEGIIALV
                     WAAAAMSYFHGIPQLNVIFNDGGAATVVNTVSVGLMGPIGGALAILGVVACPITSGDT
                     AFRSARLTIADAMNYNQDAVKNRFLIALPLFAVGVALTFIDFNIIWRYFSWANQTLAM
                     IMLWTGSAYLVKANKNHYITTLPALFMTVVTFSYIMQAKEGFRLPVNISNGIGIIVAI
                     ILGILFFKKAKHIKEQNNHKLAS"
BASE COUNT         2941 a          845 c         1408 g         2602 t
ORIGIN      
        1 ctgtttttga acatacaaat attttgaaaa atatcagttt aataataatg aatctatttt
       61 attaattttg aaattaaatt taaaataatt aatagagagg ggaaattaaa ttaggatgga
      121 aaagaaaaaa ataggcctat ggagtttggt atttatgaat gtatcagctt tatatggtat
      181 aagatggata gctaagtcta cagcatcaag ctttggttta ggattaggtg caatacctat
      241 gtggttatta ttctcattta tatactttgt accaggagct ttgatttgtg ctgaattagc
      301 ttctacatat caaaaaggtg atggaggcct ttacgattgg gttaaggaag cctttggaga
      361 aaagtatgga tttttagttt cttggttaaa ctggacagct aaaatatttt ggtattcttc
      421 atttttaact ttcttagcag taaatgtttc ttatacaata ggaaatccag ctcttgctga
      481 taataagatg tttgtactta tattatcttt aataatattc tgggcattat ctcttatagc
      541 aactaaagga ataagtttta caaaaatatt tactaataca ggtgcattag gttcaacaat
      601 accaagtgct atacttataa tattttcatt tgtagcagta tttgtgttga aaaaacatga
      661 tatagcttct acatacacaa tagcttcaat gactccaaaa cttaatatgg atgcatttgt
      721 tgctatatca gctataatgt ttggtttagc aggagcagag actgctgcta acttcataac
      781 agaaatagat aagcctgaaa agaacttccc taaggcgata ttaatatcag ctggaatagt
      841 agcttcatta tatgtattag gttcaattgc aataacaatg ataataccac cagaccaaat
      901 tactgcttct aaaggtgtat tagatgcact ttctgctgtt tgtgcgtctt taggaattgg
      961 ttcttggttg gttcaattaa tagcttttgg tatagctttt tcagtattag gagcaatagt
     1021 tctttacata gcatcaccaa taaaaatgtt atttggtagt gttaaaaaag gaatatttcc
     1081 agatagtttg gtagaagtaa atgaacacaa gataccttct aaagcagtta tacttcaagc
     1141 aataatagtt actataatac tattggtgac tacattaatg ccttctgttg atgctatata
     1201 caatgttctt gtaactatga cagcattaac tgctttattc ccatatgtat tattatatgc
     1261 ttcttatatt aaactaagaa aagagagacc agatgaaatt agaccttata ctatggcaaa
     1321 atcaacaagt acttgcctag gtttagcaaa aatggtttta gttgtaactg tagttggtat
     1381 attattatct gcagcaccag ttatgcctac atttgctgaa aatgttatat atgagataga
     1441 gatgattggt ggaggtcttc tagttatatt atctggtcta tggttatgga atagatatga
     1501 aaagaaaaca gctaaataaa atgtatttta aaaatttctt ttggatatta taatatatag
     1561 ttgacaattt atagtgtaag gaatattata aaataaagaa attaataaat taaatcctga
     1621 gataagaaat agtaaataaa tatatttttg cacagagaat taaggatgct gagaacttaa
     1681 taaaaagtat ttattgaatg ggtctttgag gagtaagatg aaatgtaatt taagattaca
     1741 gagtatttga tatcgtgtat gcacgttata gcatatatta agatatatat attcgatttt
     1801 gagtgtatat aaatttaggt ggtaccacgg aaaatatatc cgtcctattg atttaggacg
     1861 gattttttaa tatagaaaat ttaggaggaa aaatgaacgt aataggaatt ataggtgcaa
     1921 tggatgaaga agttaatatt ctagttgatt taatggatat tagagagact ataaaaaaag
     1981 ctagcttaga attttataag ggaattttgg aaggtaagaa tgtagtatta gtaaagtgtg
     2041 gcataggtaa ggttaactct gctctttgtg cccagatact aataagcgaa tttaaggttg
     2101 atgctattgt taatacaggt gttgcaggag ctttaaacga aaaacttgat gttaatgata
     2161 tagtaatatc aactgatgct atacagtatg atgtagatac tacagcattt ggagacccta
     2221 agggagttat accaagaatg aagacttctg tatttaaagc ggatgaaaga cttatagatg
     2281 ctgcttacaa atcctctgtg gaagaagtaa agactcataa agtattaaaa ggaagagtag
     2341 ttacaggaga taaatttata aattctaaag aattaaaaga agaattagta aatgattttg
     2401 gtggatattg tggagaaatg gaaggtggag ctatagctca tgtatgctat ttaaataata
     2461 ctccgttcgt aataataaga gcaatgtctg ataaggcaga tggaagcgct gatgttactt
     2521 acgatgtatt tgtacatgat gctgccaata actctaaaga tatagtttta aatatgttaa
     2581 aatcaatata aatatataag gaggtacaaa aaaatgagtg aaaaaaaagt tatatttagt
     2641 ggagcacagc catcaggcaa aatgacttta ggaaattatt taggagctat aaaaaactgg
     2701 actgaacttc aagataatta tgattgctat tatagtatag ttgatttaca tgcaataaca
     2761 gtacctcagg accctaaagt tttaagagcg aatgctatag aattacttgc tcaatatata
     2821 gcttgtggat tagacccaga aaaaaatact atatttatac aatcacatgt caaagagcat
     2881 gttgaactta tgtgggtatt aaatactatg acctatatgg gtgaattaag tagaatgact
     2941 caattcaaag ataaatcaca aaagagtgaa gcaaatctaa atgcaggttt attcacatat
     3001 ccagttctta tggctgctga catattgttg tatcaaactg acctagttcc agttggagat
     3061 gaccaaaaac aacatttaga attagctcgt gatttagcaa ataggtttaa taatagattt
     3121 tcacctactt ttgtagttcc agaaggatac tatccaaaag gtggagcaag agtgatgagt
     3181 ttgcaagagc caactaaaaa aatgagtaaa tccgattcaa atgaaaatgc atttatacta
     3241 ttagcagatg attcagattc tataaagaga aagataaaaa gaagtgttac tgattcttta
     3301 ggtgttgtaa aatacaatga tgaacaacca ggtattaaaa atctaattga tatatattct
     3361 aatttaagta aaaagagtgt agaagatatt gtaaacatgt atgaaggaaa gggttatggc
     3421 atatttaaag aagatgtggc tgaagtagta tcagaagctt taagacctat aagagaaaaa
     3481 tatgttgacc ttcttaataa taaagattat ttagaaaaaa tatatagtat gggagcagaa
     3541 aaagccgaaa aacaagctag aaaaactctt agaaaagttt ataaaaaagt tggatttata
     3601 gagagaagat actaattatt agctatcttt acttgataaa taaattttaa agttattaaa
     3661 aaagtatatg atatgaaaga gagagtctca aaggaaactt ttgttcatga ggcactcttt
     3721 tttgtatgaa ataaaatata ttttaatcat ttcaacaatg aaaaagagga ctatctctat
     3781 tttgagacag cccttttgat aataaaaaaa gatgagcttt tgctcatctc aaaggggtta
     3841 gattattctt actttgggga aagtagaata aaggtaaaag ttaactgatg tcaactttgt
     3901 cttttttgca tatcactact gagcataaaa aaatcagaaa accatagcct tctgaaacaa
     3961 cttctaacat acattgtatt ttatacccaa tatgtaattt atgagttaat tttaactctt
     4021 ataatgaaaa ttgtcaataa tatatcaaaa aaatattttt taaatttata taattaaatg
     4081 aatttatgtt tatatatagg tgatatgata caataattat ggaatttatt tttatgtatc
     4141 taacttatta caagaataat aaatttaaat taagtaatgt tttatttttt aaagggggat
     4201 tatgaaaaca tcaattttaa tcgcattaat agaaaagaca agcctcattg tagtactgtt
     4261 tcttctaata acaaagttaa aaatatttaa gcagatattt caaaaggaag aatatagttt
     4321 taatgacttg gtctgtatag cattagtttt tactttttta gccatatttg gaacttatag
     4381 tggtataaat tatatggggt caatagtaaa cactagaata atatctatag tatcaggtgg
     4441 aattttattt ggtccaatgg taggtattac agcaggagtt ttttcaggga ttcatagata
     4501 ttttatggat attgggggga taacatctgt tccatgtctt ttatcaagta tactagcagg
     4561 tgtattatca ggatttctat ataagaggat accaaaacaa catagagtaa tgtatggaat
     4621 attggtaggt atgatttctg aaagttttac tatattactt atatacttaa tatcatatcc
     4681 acattcttta gcaattcaaa taattggtgg aatatattta cctttaatag ttgggcagat
     4741 tggaatagga tttgttatat ctatagtaga ggggattgag aaagataaaa aggatataga
     4801 agcgagaaat aaagcagaaa taaaagcact tcaaagacaa attaatccgc attttttgtt
     4861 taattcccta aacacaatag cttcatttat aagatttaat ccagataagg ctagagagtt
     4921 aataataaat ttatcaactt atctaagata taatcttgaa tatagtgata acttaattga
     4981 tataaataaa gagattgaac aagttaaatc ttttgtagag atagaaaaag ctaggtttgg
     5041 agaattacta actgtatctt atgatataga agatgtaaac ataaagattc caagtttgat
     5101 tatacaacca ttagtggaaa atgcaataat acatggtatt ttagaaagtg gtagagctgg
     5161 tgtagtgaaa atatccataa aaaagttacc gtattcatta gaaaatactg tgagaatatc
     5221 aatagaggat aatggtatag gtataagtga ggaaattata aataatgtat atcaagataa
     5281 tatgccagaa aataagattg ggctttataa tgtacattta aggcttaaac tgatgtatgg
     5341 aagagggctt aatataagaa gaatagatac tgggacttta atagtatttt atgtaaagga
     5401 gtagaagtat gagagctata atagtggaag atgaatttcc agcaagaaag gaattgagat
     5461 attttattga aaataaaagt ggcatcgaag tagtaagtga atttaccaat ggtatagagg
     5521 tgttagactt tattcaggaa aataaaatag atgtaatttt tttagatata aatatacctc
     5581 atcttgatgg aatgctactt gcaaaaacat taaatcaatt taaatcaaga cctaaaattg
     5641 tttttataac agcatatgaa aattatgcag tagatgcttt ttctttggat gtatttgatt
     5701 atatattgaa accatattct gaagagagaa ttatttctat gctgaataaa ttagaaaaaa
     5761 gtgagatgag tgatattgaa ttatctaatg gaaatagtaa tttatacaaa tataagaaag
     5821 aagcagtaaa tcaggagata gaagaaataa cacataaaat tagcctttgg aaaggtgata
     5881 aattagttgt aatagatatt gatgatatat attactgtga agctaatgaa aggcaaacat
     5941 ttatatatac tgagaaagaa aagtttatat taaaagaagg catatcagaa gttgaaaatc
     6001 ttataaatga taaaacattt tttagaacac ataggtctta tatcgtaaat ttaaccaaag
     6061 taaaagaaat tataccatgg tttaataata catacatatt aaaattaaaa aatagtgact
     6121 atgaagtaac tgtaagtaga agcaaagtaa aagactttag gctgttaatg catatataaa
     6181 gtcatttcat gtacttaaat atgcacttaa tgtaattaat atttatatta gcataaaaaa
     6241 gtattactat ttgaactgga aatagttttc ttaaactcat aaaaatttat aaagcaatta
     6301 tataaaaact aaggggatga atttatggta agctttttag gttcaatagt agtacttata
     6361 gtaggttatt ttgtctatgg tacttttgta gaaaaggttt ttggaatcaa tgacaaaaat
     6421 caaactccag ctatagcttg tagggatgga gtagactatg tgccaatgaa atggaagaga
     6481 atctttttaa ttcagtttct aaatatagca gggttaggtc cgatatttgg agctatacaa
     6541 ggggcattat ttggaccatc agcattttta tggattgtgt ttggaacaat ctttgcgggt
     6601 ggagtacacg attttgcttc aggatatcta tcaatgaaga acaaaggtac atctgcatca
     6661 gagttggtag gtctttatct aggaaatggt gcaaaaatag caatgagaat atttagtgtt
     6721 attcttttag tattggttgg agttgtattt gtaacaggtc cagcaggatt attaaagaca
     6781 ttgacaggtg taaacgcaca aatatgggtt ggagtgataa tactttatta tattatggct
     6841 actgttttac caatagataa attgattggt aaaatatatc ctttatttgg agcagcatta
     6901 ttaattatgg cagtaggtgt agctggagga ttaataataa aggggtataa cataccaaat
     6961 ataaatttac aaaatatgaa tccaaatgga acacctttat tcccatactt atttataaca
     7021 atagcatgtg gagcaatatc aggtttccat gcaactcaat caccacttat ggcgagatgt
     7081 gttgagaaag agagtgaggc aagaccagta ttttatggtt caatggttgc agaaggtata
     7141 atagctttag tttgggcagc agcagccatg tcatatttcc atggtatacc tcaacttaat
     7201 gtgatattta atgatggtgg agcagcaaca gtagtaaata cagtatctgt cggattaatg
     7261 ggtcctattg gaggagcttt agctatactt ggagttgtag catgtcctat aacttctgga
     7321 gatacagcat ttagaagtgc taggctaaca atagcagatg caatgaatta taatcaagat
     7381 gcagtcaaaa atagattttt aatagcttta ccattgttcg cagttggtgt tgctcttaca
     7441 tttatagatt ttaatataat ttggagatat ttctcttggg caaatcaaac acttgcaatg
     7501 ataatgttgt ggactggttc tgcatattta gtcaaagcaa ataaaaatca ttatataact
     7561 acattaccag cactttttat gactgtagta acatttagtt atataatgca agcaaaagaa
     7621 ggctttagac taccagttaa tatatctaat ggaataggta ttattgtagc aataattctt
     7681 ggtattttat tctttaaaaa agcaaagcat attaaggaac aaaataatca taaattggca
     7741 agttagtaaa ataataacta taaaaattaa aaaattatat ttaaattatg ttcaaa
//
LOCUS       DAEPXF010000172         7780 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain HB27
            SAMN13376389-rid12479423.denovo.179, whole genome shotgun sequence.
ACCESSION   DAEPXF010000172 DAEPXF010000000
VERSION     DAEPXF010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13376389
            Sequence Read Archive: SRR10518432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7780)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7780)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001069624.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001069624.1
            Genome Coverage       :: 315x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/15/2021 01:54:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,165
            CDSs (total)                      :: 4,076
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 89
            rRNAs                             :: 10, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 10 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 19 of 69
            Pseudo Genes (incomplete)         :: 44 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 10 of 69
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7780
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13376389-rid12479423.denovo.179"
                     /strain="HB27"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="China: Hebei"
                     /collection_date="2017"
     gene            89..421
                     /locus_tag="KRL67_003163"
     CDS             89..421
                     /locus_tag="KRL67_003163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE family protein"
                     /protein_id="HBH1524010.1"
                     /translation="MKVAYIFSSTNTHKILDKMIIPQLEQGTHGVDVLGMFFFMDNTL
                     FLSKGNPVGERLSKLHEKTDMIIMACDQCAIERGIENNLVDGATIGCFPNLYACLGSA
                     GVDQVITL"
     gene            583..768
                     /locus_tag="KRL67_003164"
     CDS             583..768
                     /locus_tag="KRL67_003164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH1524011.1"
                     /translation="MSEKYADKLEIKLYQAGKDFSYIKKYGIITKGTLIINQKKKYDR
                     LNKDTIERAIVEAINNN"
     gene            795..1880
                     /locus_tag="KRL67_003165"
     CDS             795..1880
                     /locus_tag="KRL67_003165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HBH1524012.1"
                     /translation="MNILEIVLNGVKDTIVASIVTLNGASGWLMISFILAGVLHNVLT
                     PRRFQKQLGNKKFSSLIKSTISGMFLPICSCGVIPLGISMYYSGAYLGPVLAFMTSTP
                     IINPIAVVLCLGLLGPKVTIIYLIAGFLVPFLVGFLGNCFGKEELSLHNDSEEEVIEL
                     EEEKRSFMENMIDGLKWSFGELALTISKYVVMGMVFAGFITTIFPNSIIQKYLGNPGL
                     ISLGSIAILACVMYVCAVGHIPFIAALVASGASPGIAITFLIAGAATNLPELISMFKL
                     IGKRTVIIYSVTLTLSSIIIGYITNLLLVNNNSVNLDATNSSIQLANKLMLNIPDSFE
                     YVCTILIILFAFKSIIPKMKEVIGNHA"
     gene            1873..2331
                     /locus_tag="KRL67_003166"
     CDS             1873..2331
                     /locus_tag="KRL67_003166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH1524013.1"
                     /translation="MLKKIVIISCIVVVLIILSKIVDDNIKEDASIPNVNKETLEYFR
                     KNYKEDIITCAEEDLNNDGKKDLVVIYKKSNNSNEMVVVVSDKNSHYITKPIPAPIEN
                     QTITFKNIDDKVPIEVIVSGSKNGNVGYAIYRVEGKKFVDLFGEDMDKCC"
     gene            2366..3457
                     /locus_tag="KRL67_003167"
     CDS             2366..3457
                     /locus_tag="KRL67_003167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBH1524014.1"
                     /translation="MKLSKKIQALILFGLMGTSILTGCSSKPKEKEEAKAGKDDYTIT
                     VGYYNCDHMTAGPVAESAGIYKDLGLNVKTVGNGKVPEAMAAGKMDAGYIGTKGLVGA
                     IPKGSPITIAANNHTGGSEYLIVSKDIKEPKDLIGKKIATDMSDFLWTSDYGPETGLP
                     TDPSKYEVVNMDSDKDKYLALKTGKIQAFTSCDPWGSVAENDGAGKIIASTQYKEKVN
                     GKEYNCCSFSLNKNFIKEHPDLAKKLVLAHTKAIEYIYTNPAEAAKIFAKYYNVEEEV
                     ALRTIYKKTVGEGRTLTWKVTGEEYKNNLQMYKDLKALDDIPKYEDTIDTSLLDSCGA
                     DDFDKFIKEKVNPEFPEGMSYEDWKAKVL"
     gene            3462..3686
                     /locus_tag="KRL67_003168"
     CDS             3462..3686
                     /locus_tag="KRL67_003168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH1524015.1"
                     /translation="MENIKNIIILFIIILGSIFIIFFNFEDKEEIKGKLRIGVSDDTS
                     GFVINYMVNRDYFENIKVSDIMDIFTINDC"
     gene            3696..4259
                     /locus_tag="KRL67_003169"
     CDS             3696..4259
                     /locus_tag="KRL67_003169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH1524016.1"
                     /translation="MQWALSSEEIDIAIICKDAAKQYVNVNSGFEIVGTVVQNSDIFL
                     ISDNNPKKMGVIQNRDYQSELVKKYYKNVEVAPLLGTALAYGLESNLVDGVVIDCIKS
                     IGLEGKRKSTTKLGDYDTYVLVVSKEFKKSKAYSDFITLYNKSVDALKNKATFKKALK
                     EYRNIRNIDMEEISNWKLKFLKIKAID"
     gene            4220..4849
                     /locus_tag="KRL67_003170"
     CDS             4220..4849
                     /locus_tag="KRL67_003170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBH1524017.1"
                     /translation="MEIKISKDKSDRLTKKKTDGSMDILYKIILLVIIFLVWHFAAKD
                     IGSSLLLPMPVDVIKGFFFCVTDAETVTNLFITLQRVLKGFMYALLFGLPIGFIMGFS
                     KTFERVLSPVVDSVRQVPIMAWVPLTIVWFGIGDGPTIFLIAFSGVFPIILNTIQGVR
                     AISKDYYNAARSMGASPIVIFTNVIVPASLPDILTGSRIAISTGWMSVI"
     gene            5026..5778
                     /locus_tag="KRL67_003171"
     CDS             5026..5778
                     /locus_tag="KRL67_003171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBH1524018.1"
                     /translation="MKLSVRDINKTFVNNRVHTKVLEDISMDIDDGQFVCLLGPSGCG
                     KTTLLTIIGGFQKSERGDVFINDKRVKKPGIDRAFIFQNYALFPWKTIRGNVLFPMKQ
                     QKIPKEKREEMLEELLVMSDLKGKEKLFPHQLSGGMKQRVAMIRALACNPEVLLMDEP
                     LGAVDFQMRQNLQEELERIWIKKKITALMVTHDVDEAVYMSDRVIVMSRDKGRIIDDI
                     NIDIPRPRIRGSQKYEEYKNKLTDTLSKCYEV"
     gene            5791..6276
                     /locus_tag="KRL67_003172"
     CDS             5791..6276
                     /locus_tag="KRL67_003172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH1524019.1"
                     /translation="MSQIEKYNSLDNTQQKIRISIMDMIIDKGSSVTLQEVTEYISKK
                     LNIEKEYIESTLQYFIDKNIMVVDGDSINFIYPVSALPTNHKVTLRDNRSFSAMCAID
                     AIGTSCTFNQDIKINSICSATGKEIEVIIKNEKIEYVNNPNLRVLHINLDKHLNWAAS
                     C"
     gene            6283..6432
                     /locus_tag="KRL67_003173"
     CDS             6283..6432
                     /locus_tag="KRL67_003173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH1524020.1"
                     /translation="MNFFWTKSDFDAWTNEAGLSDDEDIYCLDINEAIVESYKIFKLK
                     QKVLS"
     gene            complement(6568..7389)
                     /gene="yidA"
                     /locus_tag="KRL67_003174"
     CDS             complement(6568..7389)
                     /gene="yidA"
                     /locus_tag="KRL67_003174"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HBH1524021.1"
                     /translation="MYKLIALDIDGTILNTQKRITPEVFESIQEAKRAGAKVVITTGR
                     PLPGVKELLNQLNLTDEGDYVICFNGAIIQEVKSEKIIHDVEMTLDDFDFIYNNVCKK
                     YKTKIHINTMTNLITPNETPGKYTLHEAKLNNIEVKYIQKDKIDESIKICKIMIVDEP
                     ERLEEIIQQLPKNLFNKYTIVRSAPFYLEFLGKTTNKGTALKTLCTNLNIPIENAIAV
                     GDEENDQHMIKYAGLGVAMGNARNSIKEIADYVTDTNNENGVAKVINKYILNKAI"
BASE COUNT         2993 a          858 c         1366 g         2563 t
ORIGIN      
        1 ctattttaca gataaataag aataaactta taatataatt gtatctgtaa aattttgtaa
       61 aaatataaaa agtatagggg gaataaaaat gaaagtagct tatattttta gtagtactaa
      121 tacacataaa attctagaca aaatgataat accgcaactt gaacaaggaa ctcatggagt
      181 agacgttcta gggatgtttt tctttatgga taatacctta tttttatcta aaggtaatcc
      241 tgtaggcgag agactaagta aattacatga aaaaactgat atgataataa tggcttgtga
      301 ccaatgtgct atagaaagag gtatagagaa taatttggtt gatggagcta caattggatg
      361 tttcccaaac ctatatgctt gtttaggcag tgcaggtgtg gaccaagtta taacgttata
      421 aattagtata ggatacaatc catagaaaaa tttattttct gtggattatt ttaagtaaaa
      481 gataaattgt ttaaagtagg ggatgaacaa atgtctaaaa ttttagttga atttataaac
      541 acttgaccat cttgtgatga acatggagtg tttataaaaa aaatgtcaga aaagtatgct
      601 gataaattgg aaataaaatt atatcaagca ggtaaagatt tttcgtatat aaaaaaatat
      661 gggattataa caaaaggaac tttaataata aatcagaaaa agaaatatga tagattaaat
      721 aaagatacaa ttgaaagggc aatagtagaa gctataaaca ataattaaaa gtttaaaaat
      781 taggagtatc aaaaatgaat atattagaaa ttgttttaaa tggagttaaa gatactatag
      841 tagcttctat agttacattg aatggagcat caggatggct tatgataagt tttatattgg
      901 caggtgtact tcacaatgtt ttaactccaa gaaggtttca aaaacaactt ggaaataaga
      961 aattttcttc tttaattaaa agtactatct caggaatgtt tttaccaata tgtagttgtg
     1021 gagtaatacc acttggaata agtatgtatt attcaggagc atatttagga ccagtacttg
     1081 cttttatgac atcaactcca ataataaatc caatagctgt agttctttgt ctaggtcttt
     1141 taggtcctaa agttacaatc atttatctaa tagctggttt tttagtacca tttttagtag
     1201 gatttttagg aaattgtttt ggaaaagaag aattatcact tcataatgat tcagaggaag
     1261 aagtaataga attagaagaa gaaaaaagaa gttttatgga aaatatgatt gatggtctta
     1321 aatggtcttt tggggagtta gctcttacta ttagtaaata tgtagtaatg ggtatggttt
     1381 ttgcaggatt tataacaacc atttttccaa atagtataat tcaaaaatat ttaggaaatc
     1441 caggtttgat atctcttgga agtatagcaa tacttgcatg tgttatgtat gtatgtgctg
     1501 taggacatat accgtttata gcagctcttg ttgcaagtgg agcatctcct ggtatagcga
     1561 taacatttct aatagctgga gcagctacaa atctaccaga acttataagt atgttcaaac
     1621 ttattggaaa aagaactgtt attatatatt ctgtaacttt gacactgtct tcaataataa
     1681 ttggatatat aacaaattta cttttagtga ataataactc agtaaattta gatgcaacaa
     1741 attcttcaat acagttggca aataaactaa tgttaaatat acctgatagt tttgagtatg
     1801 tatgtactat tttaattatt ttatttgcat ttaaatcaat aataccaaag atgaaagagg
     1861 ttataggaaa tcatgcttaa aaagatagtt ataatatcct gtatagtagt tgtgttaatc
     1921 attttatcaa aaatagttga tgataacatc aaagaagatg cctctattcc aaatgtgaat
     1981 aaagaaacat tagaatattt tagaaaaaat tataaagaag acataataac ttgtgctgaa
     2041 gaagacttaa ataatgatgg gaaaaaagat ttagtagtta tatataaaaa aagcaataat
     2101 tcaaatgaaa tggtagtcgt agtttctgat aaaaatagcc actatataac aaagccaata
     2161 ccagcaccta tagagaatca aactataacc tttaaaaata ttgatgataa agtaccaata
     2221 gaagtaatag tatcagggtc taaaaatggt aatgtaggat atgctattta cagagtagag
     2281 ggtaagaagt ttgttgattt atttggcgaa gatatggaca aatgttgcta aaaataaaaa
     2341 taatacataa aggtgggaaa tttatatgaa attatctaag aagattcaag cattaatctt
     2401 atttggtcta atgggtacat ctatattaac aggatgtagt agtaaaccaa aagaaaaaga
     2461 agaagcaaaa gctggaaagg atgattatac aataacagta ggttattata attgtgacca
     2521 tatgactgcg ggaccagtag cagaatcggc aggaatatat aaagatttag gtcttaatgt
     2581 aaaaactgtt ggaaatggta aagttcctga agctatggca gcaggaaaaa tggatgcagg
     2641 ttacatagga actaaaggtt tagttggagc gatacctaag ggttcaccaa taacaatagc
     2701 agctaacaac catactggtg gttctgaata tttaatagtt tcaaaagata taaaggaacc
     2761 caaagattta attggtaaaa aaattgcaac agatatgagt gatttcttat ggacaagtga
     2821 ttatggtcca gaaacaggtc taccaactga cccatcaaaa tatgaagttg taaatatgga
     2881 ttcagacaaa gataaatatt tagctcttaa aactgggaaa attcaagcct ttacatcttg
     2941 tgacccttgg ggttctgtag ctgaaaatga tggtgcagga aaaataatag catctactca
     3001 atataaagaa aaagttaatg gtaaagaata taactgttgt tcattctcat taaacaaaaa
     3061 ctttataaaa gaacatccag atttagcaaa aaaattggtt ctagcacata caaaagcaat
     3121 agaatatata tatacaaatc cagctgaagc agctaaaata tttgcaaaat attataatgt
     3181 agaagaagaa gttgcactta gaactatcta taagaagact gttggagaag gtagaactct
     3241 tacttggaaa gttacaggtg aagaatataa aaacaattta caaatgtata aagatttaaa
     3301 ggctttagat gatataccta aatatgaaga tacgattgat acaagcttat tagactcttg
     3361 tggagctgat gactttgaca aatttataaa agaaaaagtt aacccagagt ttcctgaagg
     3421 tatgagttat gaagactgga aagcaaaggt attataaata aatggagaat ataaaaaata
     3481 taataatact ttttatcatt attttgggaa gtatatttat aatatttttt aattttgaag
     3541 ataaagaaga aataaaagga aaactaagaa taggggtatc tgatgatacc tctggttttg
     3601 taataaatta tatggtaaat agagactact ttgaaaatat aaaagtaagt gatataatgg
     3661 atatttttac aattaatgat tgctgagcaa gtacaatgca gtgggctctg agttcagagg
     3721 aaatagatat agcaatcata tgtaaagatg ctgcaaaaca atatgtaaat gtaaatagtg
     3781 gatttgaaat tgttggtact gtagttcaaa attctgatat atttctaata agtgataata
     3841 atcctaaaaa gatgggagtc attcaaaata gagattacca gtcagaattg gttaaaaaat
     3901 actacaagaa tgtggaagta gctcccttat tagggactgc actagcatat ggattagaaa
     3961 gtaatttagt agatggtgtt gtaattgatt gcataaagtc aattgggtta gagggtaaaa
     4021 gaaaatctac aactaaatta ggagattatg atacctatgt attagttgta agtaaagagt
     4081 ttaaaaaaag taaagcatat agtgatttta taacacttta caataaaagt gttgatgctt
     4141 taaagaataa agctacattt aagaaagcat taaaagagta caggaatata agaaatatag
     4201 atatggagga aataagcaat tggaaattaa aatttctaaa gataaaagcg atagattaac
     4261 taagaaaaaa actgatggta gtatggatat tttatataaa ataatactat tagttattat
     4321 atttttagtc tggcattttg cagcaaaaga tatagggagt tctttattac ttccaatgcc
     4381 agtagatgta ataaaaggtt tctttttctg tgtgacagat gcagagacag tcactaatct
     4441 ttttataaca ttgcagagag tacttaaagg ttttatgtat gcattattgt ttgggcttcc
     4501 aataggattt attatgggat tttctaaaac ttttgaaagg gttttatctc cagtagtaga
     4561 ttctgtaagg caagttccaa taatggcatg ggttcctctt actatagtat ggtttggtat
     4621 aggagatggt ccaacaatat ttttaatagc tttttcaggg gttttcccta taattttaaa
     4681 tactattcaa ggagtaagag caatatcaaa ggattattat aatgcagcta gaagtatggg
     4741 agcatcgcca attgtgatat ttacaaatgt aattgttcca gcatctcttc cagatatact
     4801 aactggttca aggattgcta taagtactgg atggatgtct gttatctgag ccgagtttat
     4861 agctacgagt gccggtctcg gttactcaat ggtacaagct caaacattaa tgcatacaga
     4921 tgtattaatt gggcttatga tatttgcagc tttaatagga tttataatag atagggtatt
     4981 aaagtttata aataaaatcc tttgtagatg gaggtttgca gattaatgaa gttaagtgta
     5041 agagatataa ataagacatt tgtaaataat agagtacata caaaggtttt agaagatata
     5101 agtatggata tagatgatgg acaatttgtc tgtcttttag gaccatcagg ttgtggaaaa
     5161 acaactttac ttactataat aggtgggttt caaaaatcag aacgtggaga tgtttttata
     5221 aatgataaaa gagttaaaaa accaggcata gatagagctt ttatatttca aaattatgct
     5281 ttatttcctt ggaaaactat aagaggtaat gttctatttc ctatgaaaca gcagaaaata
     5341 ccaaaagaaa aaagagaaga aatgctagaa gaacttttgg ttatgtcaga cttgaaagga
     5401 aaagaaaaac tgtttccaca tcaattatct ggaggaatga aacaaagagt ggccatgata
     5461 agagctttag catgtaatcc agaggtatta ctaatggatg aaccattagg agctgtagat
     5521 tttcaaatga gacaaaattt acaggaagag ctagaacgaa tatggataaa gaagaaaata
     5581 actgcattaa tggtaactca tgatgttgat gaagctgtat atatgagtga tagagttata
     5641 gtaatgtcga gagataaagg aagaattatt gatgatataa atatagatat tccaagacct
     5701 agaattagag gaagccaaaa atatgaagag tacaaaaaca aattaactga tactttatca
     5761 aagtgttatg aagtttaaag aggagtagac atgagtcaaa tagaaaaata caatagtttg
     5821 gataatacac aacagaaaat aagaatctct attatggata tgataataga taaaggttct
     5881 agtgttacac ttcaagaagt gactgaatat ataagtaaaa agttgaatat agaaaaagaa
     5941 tatatagaaa gtacactaca atattttatt gataaaaata taatggttgt tgatggagat
     6001 agtataaatt ttatatatcc tgtatcagca ttgccaacaa atcataaagt aactttgaga
     6061 gataatagaa gtttcagtgc aatgtgtgct atagatgcta taggaacctc ttgcacattc
     6121 aaccaagata taaaaataaa ctctatttgt agtgctacag gtaaggaaat agaagtaatt
     6181 ataaaaaatg aaaagattga gtatgtgaat aatccgaact taagagtgct tcatataaat
     6241 cttgataagc atctaaattg ggctgctagt tgctgaaata taatgaattt cttctggacg
     6301 aagagtgatt ttgatgcttg gacaaacgaa gcgggattat ctgatgatga agatatatat
     6361 tgtttagata ttaatgaggc aatagtagaa tcatataaaa tatttaaact caaacaaaag
     6421 gtactatcat agatatacat atatgaaaaa ctaaaaatga cctttagata aaaatgatag
     6481 gcaatatccc tactatataa attagtatag agtagggatg tgaaaagtat ttaaatctaa
     6541 aggtatattt ttagtttttt tagatgatta gatagcctta ttaagtatat atttatttat
     6601 aacctttgca actccatttt cattattagt atcagtaaca taatctgcaa tttcctttat
     6661 actattacgt gcattcccca tagccacacc aagacctgca tactttatca tatgttggtc
     6721 attttcttca tcgccaacag caatagcatt ttctataggt atatttagat tcgtacataa
     6781 tgttttaaga gcagttcctt tattagtagt tttacctaaa aattctagat aaaaaggagc
     6841 agaccttact atagtatatt tattgaataa atttttaggt agctgttgta tgatttcttc
     6901 caatctttct ggttcatcaa ctatcataat tttacagatt ttaatagatt catcaatttt
     6961 atctttttgt atatatttga cttctatatt atttaatttt gcttcatgaa gtgtatactt
     7021 gccaggcgtt tcatttggtg taattaaatt tgtcattgta tttatgtgta ttttagtctt
     7081 atatttttta caaacgttat tatatataaa atcaaaatca tctaaagtca tttctacatc
     7141 atgtataatt ttctcacttt taacttcttg aataattgct ccattaaagc atataacata
     7201 atcaccttca tctgttaaat ttagctgatt tagaagttct tttacaccag gaagaggtct
     7261 gcctgttgtt ataactactt ttgccccagc cctttttgcc tcttgaatgg attcaaagac
     7321 ttcaggagta atcctttttt gtgtatttaa tattgttcca tcaatgtcta gtgcgattaa
     7381 tttgtacata tcgacctcca ataaattata tactcaaatt tcctgtgaaa acgttgacac
     7441 gactagtgtt gaacaaagtt tcagtgggaa atatggtttg ttttgtatac tattatatca
     7501 agagataagt aatttagaaa gtttaataat attaattttt tgataaaaat attacttatg
     7561 tgagataaga atgtaaaaac tttgtaatca ataattccaa aattcttaat cacaaataat
     7621 ggtatcgttt gcattgttaa tataaataaa tgtatattat atagttagaa gtttgaataa
     7681 atagaatgat ggtaatttgt tatattagtt agttttgtgt tataaaatat tgtaattcca
     7741 ttaatcatat gttattattc aacatgatta caatataaag
//
LOCUS       DAEPXF010000173         7761 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain HB27
            SAMN13376389-rid12479423.denovo.180, whole genome shotgun sequence.
ACCESSION   DAEPXF010000173 DAEPXF010000000
VERSION     DAEPXF010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13376389
            Sequence Read Archive: SRR10518432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7761)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7761)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001069624.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001069624.1
            Genome Coverage       :: 315x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/15/2021 01:54:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,165
            CDSs (total)                      :: 4,076
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 89
            rRNAs                             :: 10, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 10 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 19 of 69
            Pseudo Genes (incomplete)         :: 44 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 10 of 69
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7761
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13376389-rid12479423.denovo.180"
                     /strain="HB27"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="China: Hebei"
                     /collection_date="2017"
     gene            37..921
                     /locus_tag="KRL67_003175"
     CDS             37..921
                     /locus_tag="KRL67_003175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HBH1524022.1"
                     /translation="MKFVTFCSSNEEKIGVFNSETNSIYEINSLGLSKSYTDMNDFIE
                     NVSTGDLEKIKNNSFENAKCYKLEEVKLCSPIVRPKKDIICLGLNYKDHVNEIPDGVI
                     KNVVMPDYPIYFSKRADKIIGVDDKISLHGDLVEKLDYESELAVIIGKEGINISKEDA
                     YEYIFGYTIVNDISERALQDKHVQWFRGKSLDTHTSMGPCIVHKEEFEHTLKLDISSV
                     VNGEVRQNSNTEYFIFDIPTIINDLSRGMTLKPGDIISTGTPAGVAMGMNPQVYLKHG
                     DIVECKVEGIGVLKNIVD"
     gene            1020..2195
                     /locus_tag="KRL67_003176"
     CDS             1020..2195
                     /locus_tag="KRL67_003176"
                     /EC_number="2.1.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolecarboxamide
                     formyltransferase"
                     /protein_id="HBH1524023.1"
                     /translation="MARELELKYGCNPNQKPSKIYMKNGELPIEVLNGKPGYINFLDA
                     FNSWQLVKELKEATGLPAATSFKHVSPAGAAVGVPLSDTLKQIYFVDDLELSPLACAY
                     AMARGADRMSSYGDFIALSDVCDKETATIIAREVSDGIIAPGYTDEALEILKGKRKGN
                     YNIVKIDENYTPEPIETKDVYGITFEQGRNEILINEDLLKDIPTDNKIFTDSAKRDLI
                     IALITLKYTQSNSVCYAKDGQVIGVGAGQQSRIHCTRLAGNKADTWYLRQHPKVLNLK
                     FKKDIGRPDRDNTIDVYLSDDYMDVLADGIWQNFFEEKPEPLTGEGKRAWLKTLTGVA
                     LGSDAFFPFGDNIERAKRSGVSFIAQPGGSIRDDNVILTCNKYNIVMAFTKNRLFHH"
     gene            2323..3177
                     /locus_tag="KRL67_003177"
     CDS             2323..3177
                     /locus_tag="KRL67_003177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH1524024.1"
                     /translation="MALNIRNYSLLCNQGTAKFNEDVVGINPFGAWVLDGATGLNGKN
                     LVSTESDARWYVQWWNRYLYKNINKEESLKSIINTGIIEVKKEYELRLNGFKVEKLDL
                     PSSSIVVVKFHENKVEYFLLGDCALFIKNGDSKVIKDRSICKFDNTVFDEMSKLSNLE
                     EMAFDEIRTSVMDTIVSNRLKKNTKDGYWILDFDEEAVENSIYGYIEIKKDFQLMLTS
                     DGFTSACDRYNLIKEEELIRIAEKFGIGYIHNKVRDFEDNDYKAVKVPRFKVKDDSSC
                     LYLDIYND"
     gene            3221..4456
                     /locus_tag="KRL67_003178"
     CDS             3221..4456
                     /locus_tag="KRL67_003178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HBH1524025.1"
                     /translation="MKILHIITQKPNSTGSGIYLSGMIKGFEKIGHKQAVIAGIDVND
                     DVNCFPSEVSFYPVKYNCGELNFPVVGMSDSMPYESTRYKDLNIDMINRLKYQFKVNI
                     DKAMNDFKPELIICHHLYLLTAFVREIVKDIKVISICHGTCLRQLNTIDLEKEYIIAN
                     IRKLDLIFALHENQKYDIIKNFGVSESKVVVIGSGYNDDIFYNKNYKIKDDKIKIVFA
                     GKICKSKGLIPFIKAISKLKYSKDLIEVNFAGTGSDIESYNEIVKLASKSPFKMNFLG
                     KLEQRDLAELFNRSQIFVLPSFYEGLPVVVLEALSCGTDVITTDILGVKEWIGSEINN
                     SGKIEYVSLPFMEKEGIPKDEELYDFENNLYNAINSKIQSLLNNSNKKTSVDMSKKTW
                     DGLAYRINEVILMDELCLV"
     gene            4558..5499
                     /locus_tag="KRL67_003179"
     CDS             4558..5499
                     /locus_tag="KRL67_003179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp25"
                     /protein_id="HBH1524026.1"
                     /translation="MRISKKVLALGISALLLSVSFPTSINALDKIENIQGVDKYETAG
                     LISDKQDFTTAVLINADSTMVDGIAASGLAGVNNAAILLTNKDDIPEATLQRLNRVTK
                     IYVIGGENSISKDVEKMLLMRRMQVVRIDGVDRVDTSYKIAGEIEKIKNSDKMFLVNG
                     FKDEADAVSVASVAYRDGAPIILTKDIPSAEEDTDLDPWFGVSPVPVYAIGGKSTLSD
                     YIVSRYRATRIGGVDRYQTNKNVIEKFYNGAKEFYIASGDDLVYALVASPLAKNAPVA
                     LVSNKSDKSILSGANKVTAIGISDNSVIEQCLNAVKK"
     gene            complement(5773..5933)
                     /locus_tag="KRL67_003180"
                     /pseudo
     CDS             complement(5773..5933)
                     /locus_tag="KRL67_003180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003500201.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate transporter"
     gene            complement(6050..6514)
                     /locus_tag="KRL67_003181"
     CDS             complement(6050..6514)
                     /locus_tag="KRL67_003181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBH1524027.1"
                     /translation="MNLGNSLFQARKKHGLSQEDVAEKLGVSRQTVSKWETDETVPDI
                     RQSKKLAILYNVSLDELIDFDINIEEIQEAINKTSEETEEKINWTNAWGKKYPILLRY
                     QTDVNIPNYAYKVNMMLEELKQEYQYNELEAMLVLKDILYNVWKNRKSKRKQ"
     gene            complement(6836..7066)
                     /locus_tag="KRL67_003182"
     CDS             complement(6836..7066)
                     /locus_tag="KRL67_003182"
                     /inference="COORDINATES: protein motif:HMM:NF024076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3788 family protein"
                     /protein_id="HBH1524028.1"
                     /translation="MIDIQDKKYCLALEEVIEYVNNPVFTQFCSDMKSKYNCNEKIEF
                     SSCSWEFGWNIKLKKSGKNLCTVYLKEKYFTF"
     gene            complement(7352..7705)
                     /locus_tag="KRL67_003183"
     CDS             complement(7352..7705)
                     /locus_tag="KRL67_003183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HBH1524029.1"
                     /translation="MDRKRELKQLYKEMKFDTGVFIIKSDITKKIYLGKSNDIKSKFN
                     SLKFQLGAGSCMIKELNNEWKKYGEKAFTFEVLELIKHDDNKTEKDYLEELDILEMVW
                     LEKLKEDNTYEIYSI"
BASE COUNT         2839 a          921 c         1323 g         2678 t
ORIGIN      
        1 ataatattaa tcaacttatt gaatggagga ataaaaatga aatttgtgac tttttgtagt
       61 agcaatgaag aaaaaatagg tgtatttaat tcagaaacaa atagtatata tgaaattaat
      121 agtttaggat taagtaaatc atatactgat atgaatgatt ttattgaaaa tgtatctaca
      181 ggagatttag aaaaaataaa aaacaattca tttgaaaacg caaagtgtta taagttagaa
      241 gaagttaaat tatgttcccc aattgtaaga cctaaaaaag atattatatg tttaggtcta
      301 aactataaag accatgtcaa tgaaattcca gatggagtta taaaaaacgt agttatgcca
      361 gattatccaa tttatttttc taagagagca gacaaaataa ttggagtgga tgataagata
      421 agcttacatg gagatttggt agagaagttg gactatgaat cagaacttgc agtaatcata
      481 ggtaaagaag gtataaatat atcaaaagaa gatgcttatg agtatatatt tggatataca
      541 atagtgaatg atataagtga aagagcgctt caagataaac atgttcaatg gtttagagga
      601 aaaagcttag acacacatac atctatggga ccttgtatag tacataaaga agaatttgaa
      661 catactttaa aattagatat aagtagtgta gtaaatggag aagtaagaca aaattctaat
      721 acagagtact ttatatttga tatacctact ataataaatg atttatcaag aggaatgact
      781 ctaaagccag gagatataat ttcaactgga actccagcag gggttgctat ggggatgaat
      841 cctcaagtat atttaaagca tggcgatata gttgaatgta aagtcgaagg aataggagtt
      901 ttaaaaaata tagttgatta atggataaat atgccatata attttattag tacgaataat
      961 ttaattaata taggattaat caaatttatg attaatttat tatagagagg gatgagtata
     1021 tggcaagaga actagaatta aagtatggct gtaatccaaa tcaaaaacca tcaaagattt
     1081 atatgaaaaa tggagaactt cctatagaag tattaaatgg aaaacctgga tatataaatt
     1141 ttttagatgc atttaatagt tggcaacttg tgaaggaatt aaaagaagct acaggtctcc
     1201 cagcagcaac ttcatttaag catgtaagtc cagcaggagc agcagtagga gtacctttat
     1261 cagatacttt aaagcaaata tattttgttg atgatttaga gctttctcca ttagcttgtg
     1321 cttatgcaat ggcaagagga gcagatagaa tgtcttctta tggtgatttt atagcacttt
     1381 cagatgtatg tgacaaagag actgcaacta ttatagcaag agaagtttct gatggtataa
     1441 tagctccagg atatacagat gaagcccttg aaatattaaa aggaaagcgt aaaggcaact
     1501 ataatattgt aaaaatagat gaaaattata ctcctgagcc tattgaaaca aaggatgttt
     1561 atggaattac ttttgaacaa ggtagaaatg aaatattaat aaatgaagac ctattaaaag
     1621 atattccaac tgataataaa atttttacag acagtgcaaa acgtgattta attatagctc
     1681 ttattacatt aaaatacaca caatcaaatt ctgtttgtta tgctaaggat ggacaagtaa
     1741 taggagtagg tgcaggtcaa caatcacgta ttcattgtac tagacttgca ggtaataaag
     1801 ctgatacttg gtatcttcgt caacatccta aagtattaaa cttaaagttt aaaaaggata
     1861 taggtcgtcc agatagagat aacactattg atgtatatct ttctgatgat tatatggatg
     1921 tgcttgctga tggcatatgg caaaactttt ttgaagaaaa accagagcct ctaactggag
     1981 aaggaaaaag agcatggctt aaaactttaa caggagtagc acttggttca gatgcatttt
     2041 tcccatttgg agataatata gaacgtgcaa aaagaagtgg ggtaagtttt atagcacaac
     2101 caggtggttc tattcgtgat gataatgtta tattaacttg taataagtat aacatagtca
     2161 tggcatttac taaaaataga ttattccatc attaaaatac tgaaagtata aatataatat
     2221 agattaagta aaaatctaca acaagttttg ttgtagattt ttttatttgc tgtataattt
     2281 taaatagaac tttattttta gatattcaat ttggaggttg atatggcttt aaatataaga
     2341 aactatagcc tattatgtaa tcaaggaaca gctaaattta atgaagatgt agtcggtata
     2401 aatccatttg gggcatgggt tttagatggt gctacaggac ttaatggaaa aaatttagtg
     2461 tcaactgaaa gtgacgcaag gtggtatgtt cagtggtgga ataggtattt atataaaaat
     2521 ataaataaag aagaatcatt aaagagtatt attaatactg gaataataga agtaaaaaaa
     2581 gagtatgaac taagattaaa tgggtttaag gttgaaaaat tagatttacc atctagttct
     2641 atagttgttg taaaatttca tgaaaataaa gtagagtatt ttttattagg agattgtgcc
     2701 ttgtttatta aaaatggaga ttccaaagtt ataaaagata ggagtatttg taaatttgat
     2761 aatactgtat ttgatgaaat gagtaaactt tctaacctgg aagagatggc atttgatgag
     2821 attagaacta gtgttatgga tacaatagta agcaataggt tgaagaaaaa tacgaaagat
     2881 ggctattgga tattggattt tgatgaggaa gctgtggaaa attctatata tggatacatt
     2941 gaaataaaaa aagattttca attgatgcta acgagtgatg gttttacaag tgcatgtgat
     3001 agatataatc taataaaaga agaagaattg atacgtatag cagagaagtt tggtatagga
     3061 tatatacaca ataaagtaag agattttgaa gataatgact ataaagcggt taaagtgcca
     3121 agatttaaag taaaagatga ttcatcttgc ttatatttag atatatataa tgattagact
     3181 gtataatact atttaatttt agaaaggagg caagtgaaaa gtgaaaattc ttcatataat
     3241 aacacaaaaa ccaaatagta caggtagtgg tatatatttg tctggtatga taaaagggtt
     3301 cgaaaaaata ggtcataagc aagctgtaat tgcaggtata gatgtaaatg atgatgtaaa
     3361 ttgttttcca agtgaagtaa gtttttatcc agtaaagtat aattgtgggg agttaaattt
     3421 tcctgttgta ggtatgagtg attctatgcc atatgaaagt acaagatata aagatttaaa
     3481 tatagacatg ataaatagat tgaaatatca atttaaagta aatatagata aggctatgaa
     3541 tgattttaaa ccagaactta taatatgtca tcatttgtat ctcttaactg catttgttag
     3601 ggaaatagta aaagatataa aggttatatc tatttgtcat ggaacttgtt taagacagtt
     3661 aaataccatt gatttagaga aagaatacat aatagcaaat ataaggaaat tagatttaat
     3721 atttgctctt catgaaaatc aaaaatatga tattataaag aattttggag taagtgaaag
     3781 taaagtagtc gtaattggta gtggttataa tgatgatata ttttataata aaaattataa
     3841 aataaaggat gataaaataa aaatagtatt tgcaggtaag atatgtaaat caaagggact
     3901 gattccattt ataaaagcta tatctaaatt aaaatatagt aaagatttaa tagaagtcaa
     3961 ttttgcaggt actggaagcg atatcgaatc atataatgag atagttaaac tggcaagtaa
     4021 gtctccattt aaaatgaatt tcctaggaaa gttagaacaa agagatttag ctgagctatt
     4081 caacaggtca caaatatttg tgttaccatc attttatgag ggattaccag ttgtggtttt
     4141 agaagcttta tcttgtggca cagatgtaat cactactgat attcttggtg ttaaagagtg
     4201 gattggtagt gagattaata actctggaaa gatagaatat gtttctttac cttttatgga
     4261 gaaagaaggt attccaaaag atgaagagtt atatgatttt gaaaataatt tgtataatgc
     4321 aataaattct aagatacaat ctttattgaa taactcaaat aagaaaacat ctgtagatat
     4381 gtcaaaaaaa acgtgggatg gcttagcata tagaataaat gaagttattt taatggatga
     4441 attgtgttta gtctaaaatt atttaaatta taaaaaaagt gtaactattt ttacactatt
     4501 tttaaatgat tttatattat actaatacta aatatatttg ttttagggag gtaatttatg
     4561 aggattagta aaaaagtatt ggcgttaggg atttcagcat tattgttaag tgttagtttt
     4621 ccaacatcaa taaacgcact agataagatt gaaaatattc aaggagtaga taaatatgaa
     4681 acagctgggc ttatttcaga taagcaagat tttacaacag cagttcttat caatgcagat
     4741 tctacaatgg tagatgggat tgctgcaagt ggtcttgcgg gagtaaataa tgcagctatt
     4801 ttattaacta ataaggatga tataccagaa gcaacacttc aaagactgaa tagagttact
     4861 aagatatatg taataggtgg agaaaattct ataagtaaag atgtagaaaa aatgttactt
     4921 atgagaagaa tgcaagttgt aagaattgat ggtgtggata gagtagatac aagttataaa
     4981 attgcaggtg aaatagaaaa aattaaaaac agtgataaga tgtttttggt aaatggattt
     5041 aaggatgaag ctgatgcagt aagtgttgca tctgttgctt atagagatgg agctcctata
     5101 atattgacaa aagatatacc ttcagcagaa gaagacacag acttagaccc atggtttgga
     5161 gtttctcctg ttccagtata tgctattgga ggaaagtcta cattaagtga ttacatagtg
     5221 agtagatata gagcaactag aattggtggt gttgatagat atcaaactaa taaaaatgtt
     5281 attgaaaagt tctataatgg agcaaaagag ttctatatag caagtggaga cgatttagtt
     5341 tatgctttag ttgcatcacc acttgcaaaa aatgctcctg ttgctttagt ttcaaataaa
     5401 agtgataaga gtatattaag tggagctaac aaagtaacgg caataggtat aagtgataat
     5461 agtgttatag agcagtgttt gaatgctgta aaaaagtaat agagtgttat tagtaaaatg
     5521 aatatataca ttgaaaatta gtagattgtt cattttataa tggtataaaa ttatgataag
     5581 aggctatcaa atggagataa gcctcttttt cattgttgaa attatgaaaa tatattttat
     5641 tttatataaa aacagcgtaa ctttttgtgt atcctctcat aaatgctctc ctgcacagga
     5701 tagctacagg aagtaaggaa caggaaataa cggtagccgt caatctctca ggtaatcatc
     5761 agcagggttg ttatctgtaa tacaacgaat ggaaagaaaa ggtatcttat tcacataaca
     5821 tacatgagta tacttgctgt ttccatatct actgttaagg gtgcaaattt ttcatttatt
     5881 tttttacgtc ccttatcagt aataaaagtt tcacctgtag caattcttcc ccaaaatacg
     5941 gtttcttctt ggtttgtttc ttttacagcc atttcacgta tttttatcaa ttcacggttt
     6001 gctttaaaaa atacggtttt cagcacttta gtttccccaa aacggggatt tattgctttc
     6061 gtttgctttt tcgatttttc cagacattgt ataaaatatc tttaagaact aacattgctt
     6121 ccagttcatt atattggtat tcctgtttta attcttccaa catcatgtta accttataag
     6181 cataattagg aatattaaca tctgtctgat atctaagaag aatagggtat ttttttcccc
     6241 aggcatttgt ccaattgatt ttttcttcgg tttcctcact tgttttatta atcgcttcct
     6301 gaatttcttc tatattaata tcaaaatcaa tcaattcatc taatgacaca ttgtaaagta
     6361 ttgctaattt tttcgactga cgaatgtctg ggactgtttc atccgtttcc cattttgaaa
     6421 cagtctgcct gcttaccccc agcttttcag ctacatcttc ctgtgaaagc ccatgtttct
     6481 tccttgcctg aaataaacta tttcctaaat tcattgaaat tcctccttat ctgtttatgg
     6541 gattattata ttattttata agaacaaact ctaccaacta aaattatcat atttgctatt
     6601 tttcttaaca taattcaaag actgtattta ttctgtaaat taaaatctgc taacctctgc
     6661 gaatatcaat aagacggaaa atatcaaaat acattttttc tttatcttcc aaatcaatca
     6721 ttaactactt ttatccattc cctgtttttg tttggtacgt aaatatatct taattctggt
     6781 gtaaactcct gtaaaatcgc ttcgacaacc tctttctctt tctttccaat aacaactaaa
     6841 acgtaaaata cttttctttc agatatactg tgcataaatt ttttccagat ttttttaatt
     6901 tgatattcca gccaaattcc caactgcaag aactaaattc tattttttca ttgcaattat
     6961 atttcgattt catatccgag caaaattgag taaacacagg attgttaaca tattctataa
     7021 cttcctctaa tgccagacaa tattttttat cttgtatatc aatcatatac gaacttcttt
     7081 ctcctagtgg ttaatgtttt tttgtacatt gtagcatatt acctttatgc cgtcatttct
     7141 tttctgtttt tgaaaaaata cttattaagc ctatgatttg taaagggtgt tattcagcat
     7201 tacttacgca acaagccctt gacaaactat gtgatatagt atttatgcgg atattctcga
     7261 tttttaaacg tttttattat caaaaaaagt atctcatgaa ctaaatgtct attttgagac
     7321 actatctccc tttcttatat tatattattt tttatatgga ataaatttca taagtattat
     7381 cttcctttaa tttttctaac caaaccatct ctaaaatatc tagttcttct aaatagtctt
     7441 tctctgtttt gttatcatca tgttttataa gttctaaaac ttcaaatgta aatgcttttt
     7501 caccatattt tttccattca ttatttaatt cttttatcat acatgagcca gcaccaagct
     7561 gaaatttgag agagttaaat ttgcttttta tgtcattact ttttccaagg taaatctttt
     7621 tagtgatatc actttttatt atgaatacac ctgtgtcaaa tttcatttct ttgtataatt
     7681 gttttaattc acgttttcta tccatgattt tatatacctc tctttataat tgatatgtaa
     7741 tatgtaatta taaaaatata t
//
LOCUS       DAEPXF010000174         7712 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain HB27
            SAMN13376389-rid12479423.denovo.181, whole genome shotgun sequence.
ACCESSION   DAEPXF010000174 DAEPXF010000000
VERSION     DAEPXF010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13376389
            Sequence Read Archive: SRR10518432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7712)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7712)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001069624.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001069624.1
            Genome Coverage       :: 315x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/15/2021 01:54:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,165
            CDSs (total)                      :: 4,076
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 89
            rRNAs                             :: 10, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 10 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 19 of 69
            Pseudo Genes (incomplete)         :: 44 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 10 of 69
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7712
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13376389-rid12479423.denovo.181"
                     /strain="HB27"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="China: Hebei"
                     /collection_date="2017"
     gene            105..2333
                     /locus_tag="KRL67_003184"
     CDS             105..2333
                     /locus_tag="KRL67_003184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhgE/Pip domain-containing protein"
                     /protein_id="HBH1524030.1"
                     /translation="MRHLKKTNSLKEILNWNNIKNLKPSKGLKNILEIFKSDLKDIRG
                     NYAVLVIVLGLAFLPSLYAWFNIKASWDPYGSTANMLVAVTNEDKGNEIAGQKINVGD
                     QLIEQLKDNKSLGWQFVDEKTALEGVKKGTYYASLKIPKNFTKDLTSLITDDVKKGEI
                     IYTVNEKINAVAPKITDKGASTIQLQVNQTIVKTVSEIVLGISNGVGAGIEHSLPKLT
                     KVENSLIDLQKRFDHINKTVDLASDATSKLSDTVKDIKSDLPTIKKTLNDTKLLSGDL
                     KKFLEDTNDNLDELSPLIKSDLNLMVDLSSSASSLTLNLIDAVNSGSEDVPKLIDNLS
                     EKLSNLQSLNDTLVDFLTKLNQLTSNNRLDDVIDNLEDSSNKIDSSISTLNDIKNKVI
                     SGQQPSVSALNNVLSLSNGIGRINLNILNNFDSKISRPINSIFANSIKVANDIITVLD
                     KAEAKLPKVEEILTTSLKLSGNAQESISLIRERLPLAKGMLDDLIDTLSKISNGEDMK
                     KLVSLLESDILTKSNFLKEPVEVKTEKLYPIENYGSAMTPFYSVLSTWVGILLISALL
                     STSVHGNYKPYEIYFGRGLTFLSIALVQGFIIGTGDILLLGVTAKHPILLIVLLMFTS
                     MVFNFIVYSLVSVFGNVGKAIAIVLLVVQIGSSGGTFPIQVTPTFFQRINPIMPFTYA
                     IGATREAVGGIYAPNLTKDILVLLLFMILSICLNVLLKGPINRVIKPFNDKFGDSNLT
                     GH"
     gene            complement(2570..2869)
                     /locus_tag="KRL67_003185"
     CDS             complement(2570..2869)
                     /locus_tag="KRL67_003185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HBH1524031.1"
                     /translation="MYKNINKNQLKEMMKNEKNILLLDVRTKDEFKEYKIENAINISL
                     QELINNIDEIYDYKDKKVVVYCRSGHRSVTACNLLAEEGFEHLYNLNSGIIDYMS"
     gene            3077..6118
                     /locus_tag="KRL67_003186"
     CDS             3077..6118
                     /locus_tag="KRL67_003186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp20"
                     /protein_id="HBH1524032.1"
                     /translation="MFKQKLSKLLSSTLVLSMLFTAAPNITFADNTKDNSEKYQSSDI
                     ELHDYSKNSESYTKTKALAKEKIQTLLSKYGAVSAQYALIDNGKIEISGNGGVYSKQD
                     NKNLNKDNMYSIASISKMFTTTAVMKLVDEGKINLDTPVVNYIPEFKMADDRYKEITP
                     RMLLNHSSGLMGSSFKNTILLGDNDSYGHDNFLKELQKQRLKAKPGAFSVYCNDGFTL
                     AEILVERVSGMSFTNFLDKYINNPLNLQNTKTPENSFDSSKLAKAYVPYWEDAVPQDN
                     LNAIGAGGLYSSAENLCTFAQTFMKNSNGILSPASVKAMENKEYLNGLWPEGEDSILG
                     YGLGWDCVNTYPFNQYNLKALTKGGDSLLFHSNLIVLPDENMAVAVLSSGGSSQLNEI
                     IGQEILLSALKEKGKIKEIKPDKTFSKPQQVKMPSSLKENSGLYASSNMIKVDVNDNG
                     TLTVSSPYIENGPEDKYVYIGQDRFVSEKGNSCLKFVKEKNNITYLNMSSYDDVPGLG
                     QTASLYYVAQKVDDNNISNSVKEVWKKRNGKGYYLVDEKYTSQSYMFGSVKATLGLSD
                     ETPGYIVNTKIIDENNSNAFIEIPGVIGRDLSDIKLHKENGTEYLSFGTLTYVSEDSI
                     TNLPAEKSFTCELESNGYAKWYKIGDDIANKKIEVNLPQNSSFAVYDDKGIPVNYSLV
                     TKNNRVRLPKGGVIVFLGSPNARFEVTYQDEVNASALTGTDRYETSIKISQAGWGTAE
                     NAVLINDSAIADALAATPFAYKKNAPILLTGSSQINEKTLAELKRLKVKNVYVVGGEA
                     SINEKSLDTIKSNNISVSRISGSDRYQTSMNIAKELNNISNISKISVVNGEKGLADAV
                     SIGAVSAQNDMPIILTNENSNITEINNLFKNKKIDKSYVIGGEYTVSKNIESKLQNPQ
                     RISGSTRNETNAKVIKEFYKDSKIDNLYVAKNGMNKQDDLIDGLSVGVLAGKTKSPVM
                     LVGNSLDYNQKELFKTMRFKSVTQIGGNGNENSFKQIKDIA"
     gene            complement(6280..7554)
                     /locus_tag="KRL67_003187"
     CDS             complement(6280..7554)
                     /locus_tag="KRL67_003187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HBH1524033.1"
                     /translation="MITISHITAKNMYKSLEERINKFPQGAPPSDTLYKILNVLYTEQ
                     EAKLVAQLPIKPFRVKTAAKIWSVSESEAYRVLDKLASKALILDIEDNKGKKYIMPPP
                     MAGFFEFAMMRTRHDIDQKLLAELYYQYMNVEEDFIKDLFYSTETKLGRVYVQEEVLT
                     NENEVSILDYERATHIIDESTHIGISMCYCRHRMQHVGKACDAPMDICMTFDNVANSL
                     INNKFARRVDKIECKELLHQAYEHNLVQCGENVRKGVTFICNCCGCCCEAMVAAKRFG
                     NLHPVQTTSFIPNINHENCVKCGKCINACPIDAISKVKEDGKEYIKIDEDRCLGCGVC
                     VRNCHKNSIMLLKRDEKIITPANSVHRAVLMAIEKGQLQNLIFDNDALASHRAMGAIL
                     SAILKLEPAKKILASKQLKSVYLDKLLSMNDK"
BASE COUNT         2847 a         1082 c         1108 g         2675 t
ORIGIN      
        1 ttaaaattaa gacaaaattt taactttact atttgtgtaa aaagtaatat aatttaaaat
       61 tgattataaa tctaactttt ttctttatca aaatatttta ggaggtgaga catttgaaaa
      121 aaactaatag tttaaaagaa attttaaact ggaataatat caagaattta aaaccttcaa
      181 agggtttaaa aaatatatta gaaattttta aaagtgacct aaaagatata cgtggtaatt
      241 atgcagtact tgttatagtt ttaggtcttg cttttttacc atctctgtat gcttggttca
      301 atataaaggc atcctgggac ccttatggaa gcactgccaa tatgcttgta gcagtaacaa
      361 atgaagataa aggaaatgaa atagcagggc aaaagattaa tgtaggagac caattaattg
      421 aacaactcaa agataacaag agtttaggtt ggcaatttgt agatgaaaaa acagctcttg
      481 aaggtgttaa aaaaggtact tattatgcat cactaaagat tccaaaaaat tttacaaaag
      541 atttgacatc tttaattaca gatgatgtta aaaaaggcga aattatctat actgttaatg
      601 aaaaaataaa tgcagttgca ccaaagatta cagataaggg tgcttctaca atacaactac
      661 aggttaatca gactatagta aaaacagtta gtgaaatcgt tcttggtata tcaaacggag
      721 ttggtgcagg aattgaacac agtcttccaa aattgacaaa agttgaaaat tcccttattg
      781 accttcaaaa gagatttgac catataaata agactgttga tttagcttct gatgctactt
      841 caaaattatc tgatacagtt aaagatatta aatctgattt acctacaatt aaaaaaactc
      901 tgaatgatac aaaactgtta tcaggtgact taaaaaaatt cttagaggat acaaatgata
      961 atttagatga actttctcct cttattaagt ctgacttaaa cttaatggtt gacctctcat
     1021 cttctgcttc ttctcttaca ttaaatttaa ttgatgcagt aaatagtggc agtgaagatg
     1081 tacctaagtt gatagataat ttatcagaaa aactttctaa tttgcaatct ttaaacgata
     1141 ctttggtgga ttttttaact aaattaaacc aacttacatc aaataataga ttagatgatg
     1201 taatagataa tcttgaagac tctagcaata aaatagattc atctatatca acactaaatg
     1261 atattaaaaa caaagttata tctggacaac aaccatctgt aagtgctttg aataatgttt
     1321 taagtctatc aaatggtatt ggaagaatta atttaaatat tttaaataat tttgactcaa
     1381 aaatatcaag acctataaat agtatatttg caaacagtat aaaagtagca aatgatataa
     1441 ttactgtttt agataaggca gaagcaaaac ttccaaaggt tgaagaaata cttactactt
     1501 ctctaaaact ttcaggaaat gcacaggaaa gtatatcatt gataagagaa agacttcctt
     1561 tagcaaaagg gatgttggat gatttgattg atactttaag caagattagt aatggtgaag
     1621 atatgaaaaa attagtcagt ttgcttgaaa gtgatatatt aacaaagtct aatttcttaa
     1681 aagaacctgt tgaggtgaaa acagagaaat tatatccaat tgaaaattat ggttctgcaa
     1741 tgacaccatt ttatagtgtt ctttctacat gggttggtat attacttatt tcagcactat
     1801 tgtctacatc tgtacatgga aactataaac cttatgaaat ttactttgga agaggcctta
     1861 cttttttaag tatagctctt gttcaaggtt ttataatcgg aactggagat atattattat
     1921 taggagtaac agcaaaacat ccaattttat tgatagttct gcttatgttt actagtatgg
     1981 tatttaactt tatagtgtat tctttagttt ctgtatttgg aaatgtagga aaagcaattg
     2041 caatagtact attagtcgtt cagattggtt cttctggtgg tacattccct atacaagtta
     2101 cacctacatt cttccaaaga ataaacccta taatgccatt tacctatgca ataggtgcta
     2161 caagggaagc agttggtggt atatatgctc caaatctaac taaagatatt ttagttctat
     2221 tactatttat gattctgtct atttgtttaa atgtacttct aaaaggacct ataaatagag
     2281 ttataaaacc atttaatgat aagtttggcg atagtaattt aactggtcat taaaaaatta
     2341 attctgtatt taaatatatt ttttaataat attctaagtc catataaaaa ggtagtagct
     2401 aaaaagctac taccttttta tatcttaaaa actaatactt actacaaaaa ctgtcataga
     2461 aaaaagcgaa aaaattcgtt ctttcctaaa tttatcttat aaatatttag atatattcct
     2521 acttaataag cagagctata catagtattt aatgtataac ttacattatt taactcatat
     2581 aatcaattat tcctgagttt aaattatata aatgctcaaa accttcttca gctaacaaat
     2641 tacatgcagt aacacttcta tgaccacttc tacaataaac tactactttc ttatctttat
     2701 aatcgtaaat ttcatctata ttattgatta gttcttgtaa ggatatattt atagcatttt
     2761 ctattttata ttccttaaat tcatctttag ttcttacatc caataataaa atattctttt
     2821 catttttcat catttcttta agttgattct tatttatatt cttgtacata tgtctaaaat
     2881 tcctccattt ttatttatat attacctata ccctttattc tttatacaac aacaaatatt
     2941 atttttatat ttttctctgt tatataaaaa gctatactta taaaagcttt ttttattgat
     3001 aataatctgt tgattaaaat aattaagaat gttatgctaa ttataaatta ttaaaaaggg
     3061 aaagaggtaa tgtaatatgt ttaagcaaaa gctctctaaa ttattatcta gtacattagt
     3121 attgagtatg ctatttactg ctgctccaaa tattactttt gctgataata caaaagataa
     3181 ttccgaaaaa tatcaaagtt ctgatataga attacatgat tatagtaaaa attcggaatc
     3241 ttacacaaaa actaaagctc ttgccaaaga gaaaatccaa actttgctat caaagtatgg
     3301 tgctgtaagt gctcaatatg ccttgattga taatggtaaa attgagattt ctggtaatgg
     3361 tggagtttac agcaaacaag ataacaaaaa cttaaataaa gataacatgt atagtatcgc
     3421 atctatcagt aaaatgttta ctacaacagc tgttatgaag ctggttgatg agggaaaaat
     3481 aaatttagat actcctgttg taaattatat acctgaattt aaaatggctg atgatagata
     3541 caaagaaatc actccaagaa tgttattaaa tcattcttca ggattgatgg gaagtagttt
     3601 taaaaatact attttattag gagacaatga ttcttatggc catgataatt tcttaaaaga
     3661 attacaaaaa caaagattaa aagctaaacc aggagctttt tctgtttatt gtaatgatgg
     3721 atttacttta gctgaaattt tagtagagag agtatctggt atgtcattta ctaattttct
     3781 tgataaatat ataaataatc cacttaactt acaaaacaca aaaactccag aaaatagttt
     3841 tgacagttca aaactagcaa aagcatatgt accttattgg gaagatgcag ttccacaaga
     3901 taaccttaat gctataggtg caggtggttt atattcaagt gcagaaaacc tatgtacatt
     3961 tgcacaaaca tttatgaaaa actcaaatgg tattctttct cctgcttcag taaaagctat
     4021 ggaaaacaaa gaatatttaa atggtctatg gcctgaagga gaagactcta tacttggata
     4081 tggtttaggg tgggattgtg tcaacactta tccatttaat cagtataatt taaaagctct
     4141 tacaaaaggt ggagactctt tattattcca cagtaatctt atagttcttc cagatgaaaa
     4201 tatggcagtt gcagttcttt cttctggtgg aagtagtcaa cttaacgaaa taattggaca
     4261 agaaatctta ctatctgcat taaaagaaaa aggtaaaatt aaagaaataa aacctgataa
     4321 aacatttagt aagccacaac aagttaaaat gccaagctct ttaaaagaaa atagtggtct
     4381 ttatgcttct tcaaatatga taaaagttga tgttaatgac aatggtactt taacagtatc
     4441 ttctccttat atagaaaatg gacctgaaga taaatacgta tacataggtc aagatagatt
     4501 tgtatctgaa aaaggtaata gttgtttaaa atttgtaaaa gaaaaaaaca atatcacata
     4561 tttaaatatg agctcttatg atgatgtccc tggattaggt cagacagcaa gtttatacta
     4621 tgtagctcaa aaggtagatg acaacaatat atcaaatagt gtaaaagaag tgtggaaaaa
     4681 aagaaatggc aaaggttact atctagtgga tgaaaaatat acttctcaaa gctatatgtt
     4741 tggttctgtg aaggcaactc ttggtctttc tgatgaaact ccaggttata tagttaacac
     4801 taagataata gatgagaata actcaaatgc ttttatagaa atacctggtg taataggtcg
     4861 tgatttatcc gacattaaac ttcataaaga aaatggtaca gagtatttat cttttggcac
     4921 actaacgtat gttagtgaag attctataac taatttgcca gcagaaaaat cttttacttg
     4981 tgaactagaa tccaatggtt atgcaaaatg gtataagatt ggtgacgata tagcaaataa
     5041 aaaaatagaa gttaatcttc ctcaaaattc atcttttgct gtttatgatg ataaaggtat
     5101 tcctgtaaac tattctttag ttacaaagaa caatagagtt agattgccaa aaggaggcgt
     5161 aattgttttc ttaggaagtc caaatgctag atttgaagtt acatatcaag atgaggtaaa
     5221 tgcaagtgca cttacaggaa ctgatagata tgaaacttct attaagataa gtcaagcagg
     5281 ttggggaact gctgaaaatg cagttttaat aaacgactca gcaattgcag atgctttggc
     5341 tgcaacacca tttgcataca agaaaaacgc acctatatta ttgactggaa gctctcaaat
     5401 aaatgaaaaa actttggctg aattaaagag attaaaagtt aaaaatgtat atgtagttgg
     5461 tggagaggct tctataaatg aaaaatcttt agatactata aaatcaaaca atatctctgt
     5521 atctagaatt tcaggaagtg atagatatca aacttctatg aacattgcta aagaattgaa
     5581 taatattagc aatatatcaa agatttcagt agttaatggt gaaaaaggac tagccgatgc
     5641 agtaagcatt ggagctgtat cagctcaaaa tgatatgcct ataatactta ctaatgaaaa
     5701 tagtaatata actgaaataa ataacttgtt taaaaacaaa aaaattgata aatcttatgt
     5761 aatcggtgga gaatatacgg tttctaaaaa tatagaaagt aaacttcaaa atccacagag
     5821 aatatcaggc agcactagaa atgaaactaa tgcaaaagta ataaaagaat tttacaagga
     5881 ttctaagata gacaacttat atgttgctaa aaatggaatg aataaacaag atgatttaat
     5941 agatggatta tctgttggag ttttggctgg aaaaactaaa tctcctgtaa tgctagtagg
     6001 aaactcacta gattataatc aaaaagaact atttaaaact atgagattta aatctgtaac
     6061 acaaattggt ggaaatggaa atgaaaactc atttaaacaa ataaaagaca tagcttagca
     6121 taactaaaaa aggttatatt caagtaacat taattgaata taaccctttt ttatttcagt
     6181 tataaatttt atatgtgtaa agataaaatt tatatattac atgataagtt tattaaaata
     6241 taaataagtc taaaaacttt gcctttttta agttagtttc tatttgtcat tcatacttaa
     6301 caatttatct aaatatactg actttaattg tttactagct aaaatctttt tagctggttc
     6361 aagctttagt atagctgata gtatagctcc catagctcta tgactagcta gagcatcatt
     6421 atcaaaaatt aaattttgta actgcccttt ttcgattgcc atgagaactg ctctatgtac
     6481 tgaatttgct ggagttatta ttttttcatc tctctttaat aacataatgc tatttttatg
     6541 gcaatttcta acacaaactc cacaacctag gcatctatcc tcatcaattt ttatatattc
     6601 tttaccatct tccttaacct tgcttatcgc atctattgga catgcattta tacatttacc
     6661 acactttaca caattttcat ggtttatatt tggtataaaa ctggttgtct gtactggatg
     6721 aagatttcca aatcttttag ctgctaccat tgcttcacaa caacatccac aacaattaca
     6781 tataaacgta accccttttc taacattttc tccacattga actaagttat gctcatatgc
     6841 ttggtgaagt agttctttac attctatttt atccacgcgt cttgcaaatt tattatttat
     6901 taaagaattt gctacattgt caaacgtcat acatatatcc ataggagcat cacaagcttt
     6961 tccaacatgt tgcattctat gtctacagta gcacatactt atacctatat gagttgattc
     7021 atcaattata tgagtagccc tttcataatc taaaatacta acctcattct cattagttaa
     7081 aacttcctct tgaacatata ctcttccaag cttcgtttct gttgaataaa ataaatcttt
     7141 gataaaatct tcttctacat tcatatattg atagtaaagt tctgctaaaa gtttttggtc
     7201 tatatcgtgt ctcgttctca tcatagcaaa ctcaaaaaaa ccagccattg gaggaggcat
     7261 tatatatttc ttccctttat tatcttctat atctaatatc agagctttac ttgcaagttt
     7321 atccaatact ctatatgctt cactttcact tacactccaa attttagctg ctgtttttac
     7381 tctgaaaggc tttattggaa gctgagcaac taacttcgcc tcttgttctg tatatagtac
     7441 atttagtatc ttgtataatg tatctgatgg tggagcacct tgtggaaact tgtttattct
     7501 ttcctctaaa cttttataca tattcttagc agttatatga gaaatagtaa tcaccttctt
     7561 tttcttttgt tttccaataa aattaaatat tctttcttct tatatagact cttactttat
     7621 ataataccaa ttctttctaa atagttaaat acctctagca atttattatt agatttctta
     7681 tataaataaa aaaatcgttt aagtagtata at
//
LOCUS       DAEPXF010000175         7705 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain HB27
            SAMN13376389-rid12479423.denovo.182, whole genome shotgun sequence.
ACCESSION   DAEPXF010000175 DAEPXF010000000
VERSION     DAEPXF010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13376389
            Sequence Read Archive: SRR10518432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7705)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7705)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001069624.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001069624.1
            Genome Coverage       :: 315x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/15/2021 01:54:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,165
            CDSs (total)                      :: 4,076
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 89
            rRNAs                             :: 10, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 10 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 19 of 69
            Pseudo Genes (incomplete)         :: 44 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 10 of 69
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7705
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13376389-rid12479423.denovo.182"
                     /strain="HB27"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="China: Hebei"
                     /collection_date="2017"
     gene            47..1105
                     /locus_tag="KRL67_003188"
     CDS             47..1105
                     /locus_tag="KRL67_003188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium channel family protein"
                     /protein_id="HBH1524034.1"
                     /translation="MNCKWISKIDERKKCHREADSSGYCIFHKENKSDEEIQLMMDTL
                     HQEEISEFNGFVFENEFNAEEILTYYYKILDFSESIFKQKANFKKYIFKKNIIFNYTE
                     FRDKVLFNGCVFLENCDFNRTIFSKEYINDHIFEKVKFKGPDLVVNKVENFPRMDGII
                     FSMCTKFVLKNVEYGKSEYEHGKINYRIARNQATKIGEYEMIGFYYYKERIYSSKIMK
                     RSNYPTFSDYLVEKFFDQIARYTTGYGEKPWNILLVIIAIISVFALLYLFVGIESSNS
                     TLVALDINNIGDYSLSEIFRMYMDLWYFSMATFSTVGYGDMVATSLIGKALAGIEVFF
                     GVTIGAIWASVIIKRMIR"
     gene            1209..2393
                     /locus_tag="KRL67_003189"
     CDS             1209..2393
                     /locus_tag="KRL67_003189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HBH1524035.1"
                     /translation="MKNALKKIISLAMILVFIIPNYSYADGKSVDKYSKTSILIDQET
                     GRVLYSKEPDMKVPLASLSKMMTFLLAIEAIENKEVKETDKVKIDKSIASVKGSSYKL
                     KDGEEVPLIELMRGLMIVSGNDAAIAIAKHICKTKEAFVDRMNKKAKEIGMSNTHFLN
                     PNGLPIYDLSNPKKPAKENISTAKDIAILGKYMFDHYEKQVTAITDMETYTNSDRSFE
                     KSNTNALLRIIPEVDGIKTGYTGNAGYCLSFSMVVNKNDKNEKKRRVIGVVLGTNHKN
                     KRTSASLALLKYGKENFNMKKVIDKDSFIGKKYIKGMKELEVTLKAKDELYTILKDDE
                     SIKSEVKIKNIEYPVKKGDTMGIIKYYTSSGDLLGSVDIVSNNSIDNISLKTKLKMKF
                     AN"
     gene            2600..3313
                     /gene="rpsB"
                     /locus_tag="KRL67_003190"
     CDS             2600..3313
                     /gene="rpsB"
                     /locus_tag="KRL67_003190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HBH1524036.1"
                     /translation="MSVISMKQLLEAGVHFGHQTRRWNPKMAKYIFTERNGIYIIDLQ
                     KTVKKVEEAYKFTKEVAETGKPILFVGTKKQAQDAIKDEAERCGMYFVNERWLGGMLT
                     NHKTIKTRINKLRELEKMEEEGVFNVLPKKEVIKLRAEKEKLEKYLGGIKDMPELPGA
                     MFVVDPRKENIAIQEAHRLGIPVVGIVDTNCDPEQLDFAIPGNDDAIRAVKLITGAMA
                     TAVIEGRQGAEEEVAEDQE"
     gene            3392..4303
                     /locus_tag="KRL67_003191"
     CDS             3392..4303
                     /locus_tag="KRL67_003191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HBH1524037.1"
                     /translation="MANITAQMVKELRESTGAGMMDCKKALQEAEGNMEKAVDLLREK
                     GLSKAAKKAGRVAAEGLVAIEMNDDNTVASMVEVNSETDFVAKNEDFKVFVKDAACMA
                     LATDKEDIASLLGETHKEGITLQEVLNNRVAKIGEKLDFRRFAKVVTNGQVAGYIHGG
                     GKIGVLVEMETEARDAKVLELGKDVAMQVAAMNPKYVSRDEVDAEYIAHETEVLTQQA
                     LNEGKPANIVEKMVKGRLEKELKEVCLLEQTFVKNPDITVKQLVADVAKAVGSDIKVV
                     KVVRFEVGEGIQKREENFAEEVAKQLK"
     gene            4387..5091
                     /locus_tag="KRL67_003192"
     CDS             4387..5091
                     /locus_tag="KRL67_003192"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HBH1524038.1"
                     /translation="MDKPMYKRVLLKLSGEALAGERGFGINNDVVNDIAIAIKKIQEI
                     GVEVAVVVGGGNFWRGRTSEGMDRTTADYIGMLATVMNAMALQDALENIDVATRVQTA
                     IDMRQIAEPYIRRRAVRHLEKERVVIFGAGTGNPYFTTDTTAALRAAEMEAEVILLAK
                     NVDAVYDKDPKVHADAKKFTELSYMEVIQKELKVMDSTATSLCMDNKIPIKVFELTTE
                     NIIRAVKGENIGTTVK"
     gene            5117..5674
                     /gene="frr"
                     /locus_tag="KRL67_003193"
     CDS             5117..5674
                     /gene="frr"
                     /locus_tag="KRL67_003193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HBH1524039.1"
                     /translation="MKLEIHKQLEEKMNGTIDALKFEFGTIRAGRANAQMLDKIRVDY
                     YGTPTPINQIGAISVPEPRILMISPWDKSAMHEIEKAIANSDLGLNPSNDGEVIRLSV
                     PALTEERRKELAKKASKAAEEFKVRIRNERRDANEKIKKMEKGGELTEDELKKAQDEV
                     QKMTDKFIKEIDTLLSKKEKDIMEV"
     gene            5677..5880
                     /locus_tag="KRL67_003194"
     CDS             5677..5880
                     /locus_tag="KRL67_003194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH1524040.1"
                     /translation="MKKYYNLLGLHVDEVKQYFDKQNINYTIKSIEGKKDKEKLVVPR
                     VIKISEIGDSVELIITYFSDSLV"
     gene            5909..6643
                     /locus_tag="KRL67_003195"
     CDS             5909..6643
                     /locus_tag="KRL67_003195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HBH1524041.1"
                     /translation="MNNNIIYDIDLNNIPTHIAIIMDGNGRWAKARFLPRTAGHKAGV
                     ETIRDIVKECSKLGVKHLTLYAFSTENWKRPKLEVDTLMNLLSTYLRNEIAELHQNNV
                     KVTAIGDISALPKTCIRELNSAKELTKNNTGVNLNLALNYGSRADIKNALIDIVKNCE
                     SGKIDINNIDEDIIKNYLSTKSIPDPDLVIRTSGEQRLSNFLLWEVAYSEFYFTDIHW
                     PDFKKEELQKAIYVYQRRDRRFGGLK"
     gene            6656..7435
                     /locus_tag="KRL67_003196"
     CDS             6656..7435
                     /locus_tag="KRL67_003196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HBH1524042.1"
                     /translation="MLTRIIASLALVPLFLFVVYGGVPLYIAETAIVYIALHEFYKAF
                     KIKDVHPIFIIGYLFSIYLAVKNIFNLPLEYTYAVIFILFLASIIYMLMGKNNVIDVS
                     ITFLGVFYIGVFLDFIIITINGFEKGSIYVWLIFVISFMTDIFAYFSGYLLGKHKLIP
                     KVSPKKTIEGAIGGIIGSTLCCILFGYLFGIDLLQLAIIGSIGSVIAQLGDLFASSIK
                     RYVGIKDYGKIIPGHGGILDRFDSVILVAPFVYSAIKFFIR"
BASE COUNT         3169 a          784 c         1461 g         2291 t
ORIGIN      
        1 aaactaatac aaaatagtaa taattgtttt gtactggagg atatttatga attgtaaatg
       61 gatatctaaa atagatgaga gaaaaaaatg ccatagagaa gcagattctt ctggatattg
      121 tatctttcat aaagaaaata agtctgatga agagatacag ttgatgatgg atactcttca
      181 tcaagaagag ataagtgaat ttaatggatt tgtttttgaa aatgaattta atgcggaaga
      241 aatattaact tactattata aaatactgga tttttctgaa tccatattta aacaaaaagc
      301 aaattttaaa aaatatattt ttaaaaagaa tattattttt aactatacag aatttagaga
      361 taaagtttta tttaatggtt gtgtattctt agaaaactgt gattttaata gaaccatatt
      421 tagtaaagaa tatataaatg accatatatt tgaaaaagtg aaatttaaag gacctgattt
      481 ggtagttaat aaagtagaaa attttccacg gatggatgga ataattttta gcatgtgtac
      541 aaaatttgtg ttaaagaatg ttgaatatgg aaaaagtgag tatgagcatg gaaaaataaa
      601 ttatagaata gctagaaatc aagcaacaaa aattggagaa tatgagatga taggatttta
      661 ctattataag gaaagaattt atagtagtaa aataatgaaa cgttctaatt atcctacttt
      721 cagtgattat ttagttgaaa aattttttga ccaaatagca cgttatacga caggttatgg
      781 agaaaagcca tggaatatat tattagtgat tatagcaata ataagtgttt ttgctttatt
      841 atacttattt gtaggcatag aaagttcaaa tagtacactt gtagctttag atataaacaa
      901 tataggtgat tactcactaa gtgagatttt cagaatgtat atggatttat ggtattttag
      961 catggccaca tttagtacag taggatatgg agatatggtt gctacaagtt tgattgggaa
     1021 agcattggca ggtatagaag tattttttgg agttacaata ggagcaattt gggcatctgt
     1081 gattattaaa agaatgataa ggtaatattt gtgtaaatat ggataggtat taattactag
     1141 atataaattg gactaaatag ggaaaattta aatataaata tacttaataa tccaagaagg
     1201 tgaaaaattt gaaaaatgca ctaaaaaaaa ttatatcatt agccatgatt ctagttttta
     1261 taataccaaa ttattcatat gcagatggta aatctgtaga caagtattca aaaacatcta
     1321 tacttataga ccaagaaaca ggtagagttt tatatagtaa agaacctgat atgaaagtac
     1381 cattagctag tttaagtaag atgatgactt ttttacttgc aatagaagct attgaaaata
     1441 aagaagtaaa agaaactgat aaggtaaaaa tagacaaatc tattgcatca gtaaaagggt
     1501 caagttataa acttaaagat ggtgaagaag tgccacttat agagcttatg agaggtctta
     1561 tgattgtatc aggaaatgat gcggcaattg ctatagcaaa acatatttgt aaaacgaaag
     1621 aagcatttgt agatagaatg aataaaaaag caaaagaaat aggaatgagc aatacacatt
     1681 ttctaaatcc aaatggtctt ccaatatacg atttaagcaa ccccaaaaaa ccagcaaaag
     1741 aaaatatatc tactgcaaaa gatatagcta ttcttggaaa atacatgttt gaccattatg
     1801 aaaaacaagt aacagctata actgatatgg agacatatac aaattcagat agaagttttg
     1861 aaaaaagcaa tacaaatgca ttattaagaa ttataccaga agtagatggt ataaaaactg
     1921 gatatactgg caatgctgga tattgtttat cattttctat ggttgtaaat aaaaatgata
     1981 aaaatgagaa aaaacgtaga gtaatagggg ttgtattagg tactaatcat aaaaataaaa
     2041 gaacctctgc ctcattagct ctactaaaat atggaaaaga aaatttcaat atgaaaaaag
     2101 taattgataa agatagcttt attggtaaga aatatataaa aggaatgaaa gaactagagg
     2161 ttacattaaa agcaaaagat gaactttata caatattaaa agatgatgaa agtataaaat
     2221 cagaagttaa gattaaaaat atagagtacc ctgttaaaaa aggcgatact atgggtatta
     2281 taaaatatta tactagttca ggtgacttac ttggaagtgt agatatagta agtaataata
     2341 gtatagataa tatatcatta aagacaaaat taaaaatgaa atttgcaaat taaattcaaa
     2401 aaatggtata aaatacacaa aaaaatataa aaatagaaaa tatggaaaaa atattgttat
     2461 ttcttcacct ctgtgatata ctactagagg tgaaaaaata aaataaatca cacacagcat
     2521 tgggatcctt aaaaggtgcc tattacatgg ttttttaggg agaagatcaa gctggaggta
     2581 taaaaccagg aggtaagata tgtcagtaat atcaatgaaa caattattag aagcaggtgt
     2641 tcactttggt catcaaacaa gaagatggaa ccctaaaatg gctaagtata tattcacaga
     2701 gagaaatgga atatatataa tcgacttaca aaaaacagtt aaaaaagttg aagaagctta
     2761 caagttcact aaagaagtag ctgaaacagg aaagccaatc ttattcgtag gaactaaaaa
     2821 acaagctcaa gatgcgataa aagatgaagc tgaaagatgt ggaatgtact ttgttaatga
     2881 aagatggtta ggtggaatgt taacaaacca caaaactata aaaacaagaa taaataaatt
     2941 aagagaatta gaaaaaatgg aagaagaagg cgtatttaat gttcttccta aaaaagaagt
     3001 tataaaatta agagctgaaa aagaaaaact agaaaaatac ttaggtggaa taaaagacat
     3061 gcctgaatta ccaggtgcaa tgttcgtagt tgacccaaga aaagaaaaca tagctattca
     3121 agaagctcat agattaggta taccagtagt tggtatagtt gatactaact gtgacccaga
     3181 acaattagac tttgctatac caggaaatga tgacgctata agagccgtaa aattaataac
     3241 tggtgctatg gcaacagctg taattgaagg tagacaaggt gctgaagaag aagtagctga
     3301 agaccaagaa taataatttg acaattaaat ggtaagggtc gcccttacca tttataatat
     3361 ggaatagaat aagtaggagg aaacttaaaa aatggctaat ataactgctc aaatggtaaa
     3421 agagttaaga gaaagtacag gcgcaggaat gatggactgc aagaaagctt tacaagaagc
     3481 tgaaggaaac atggaaaaag cagtagattt attaagagaa aaaggattat caaaagctgc
     3541 taaaaaagct ggtagagttg ctgctgaagg tctagttgca atagaaatga atgatgataa
     3601 tacagttgct tctatggtag aagttaactc agagacagat ttcgttgcta aaaacgaaga
     3661 cttcaaagtg ttcgttaaag atgctgcttg tatggcatta gctactgata aagaagatat
     3721 agcttcttta ttaggtgaaa ctcataaaga gggaataact ttacaagaag ttttaaataa
     3781 tagagttgca aaaataggtg aaaaattaga ctttagaaga tttgctaaag ttgtaactaa
     3841 tggacaagtt gctggataca ttcacggtgg tggaaaaata ggtgttcttg ttgaaatgga
     3901 aacagaagct agagatgcta aagttttaga attaggtaaa gatgtagcta tgcaagttgc
     3961 tgctatgaat cctaagtatg tttcaagaga tgaagtagat gctgaataca tagcacatga
     4021 aacagaagta ttaactcaac aagctttaaa tgaaggaaaa ccagctaata tagttgaaaa
     4081 aatggttaaa ggaagattag aaaaagaatt aaaagaggtt tgtttattag agcaaacttt
     4141 cgttaaaaat ccagatataa ctgttaaaca attagttgct gatgttgcta aggcagtagg
     4201 ttctgatata aaagttgtta aagttgtgag attcgaagtt ggtgaaggta tacaaaagag
     4261 agaagaaaac tttgcagaag aagttgctaa gcaacttaag taataaccaa agagagaaca
     4321 cgcaggtgtt ctctttttta aaaaatagaa aaaatggata tgaaggattg aaataggagg
     4381 tttctgatgg ataaaccaat gtacaaaagg gtattgttaa aattaagtgg agaagcatta
     4441 gcaggagaaa gaggttttgg tataaataat gatgtagtta atgatattgc tattgctata
     4501 aaaaaaatac aagaaatagg tgttgaagta gctgtagtag taggtggagg aaacttctgg
     4561 agaggtagaa ctagtgaagg aatggataga actactgctg actatatagg aatgttagct
     4621 acagttatga atgcaatggc gctacaggat gcactagaaa atatagatgt tgctacaaga
     4681 gttcaaactg ctatagacat gagacaaatt gcagaaccat atataagaag aagagcagtt
     4741 agacacttag aaaaggaaag agttgttata tttggtgcag gaacaggaaa cccttacttt
     4801 acaacagata caactgctgc tttacgtgct gctgagatgg aagcagaagt tatattgtta
     4861 gctaaaaatg ttgatgcagt atatgacaag gatccaaaag tacatgcaga tgctaagaaa
     4921 tttacagaat taagttacat ggaagttata cagaaagaat taaaagtaat ggattctaca
     4981 gctacatctt tatgtatgga taataaaatt ccaataaagg tatttgagct tacaacagag
     5041 aatataataa gagcagtaaa aggcgaaaat ataggaacta ctgtaaaata atgaagaatg
     5101 aaggaggagt aaaattatga aactggaaat acataagcaa ttagaagaga aaatgaatgg
     5161 gacaattgat gctttaaagt ttgaatttgg aactataaga gcaggaagag ctaatgcaca
     5221 aatgctagac aaaataagag ttgattatta tggaactcca actccaataa atcaaatagg
     5281 tgctatatct gtaccagaac caagaatatt aatgatatct ccttgggata aatctgctat
     5341 gcatgaaata gaaaaagcga tagctaactc agacttagga ttaaatccat caaatgatgg
     5401 tgaggttata agactttctg tacctgcttt aacagaagaa agaagaaaag aattagctaa
     5461 aaaagctagt aaagctgcag aagaatttaa agttagaata agaaatgaaa gaagagacgc
     5521 caatgagaaa attaaaaaga tggaaaaagg cggagaatta actgaagatg aattaaaaaa
     5581 agcacaagat gaagttcaaa aaatgactga taaatttata aaagaaatag atacattatt
     5641 atctaaaaaa gaaaaggaca taatggaggt ataatattga aaaaatatta taacctttta
     5701 ggtcttcatg tagatgaggt aaaacaatat tttgataagc aaaatataaa ttataccata
     5761 aaatctattg aaggtaaaaa ggataaagaa aaacttgtag ttcctagagt tataaaaata
     5821 tctgagatag gtgatagtgt agaactgata atcacatact tttctgactc cttagtttaa
     5881 ggagtcagaa caatattgga ggcaaaatat gaataacaac attatatatg atatagactt
     5941 aaataacata cctactcata ttgctattat aatggatgga aatggaagat gggcaaaagc
     6001 tagatttctt ccaagaactg caggacataa ggctggagta gaaactataa gagatatagt
     6061 aaaagaatgc tcaaaattag gtgttaagca cttaacatta tatgcatttt caacagaaaa
     6121 ctggaaaaga ccgaaacttg aagttgacac acttatgaac ttattatcta catacttaag
     6181 aaatgaaata gctgaacttc accaaaacaa tgtaaaagta actgctatag gagatataag
     6241 tgctttacca aaaacttgta taagagaatt aaactcagct aaggaactga caaaaaacaa
     6301 tacaggagtt aacttaaatt tagcattaaa ctacggaagt agagcagata taaaaaatgc
     6361 tcttatagat atagtaaaaa attgtgaaag tggtaaaata gatataaaca atattgatga
     6421 agatattata aaaaactatt taagcacaaa gtctatacca gatccagact tggtaataag
     6481 aacaagtggt gaacaaagat tgagtaactt tttactttgg gaagttgctt attctgagtt
     6541 ttactttact gatatacatt ggcctgattt taaaaaagaa gaactacaga aggctatata
     6601 cgtatatcaa agaagggata gaagatttgg aggacttaag taaggagatt attttatgct
     6661 tacaagaatt attgcttcac tagctctggt tccattattt ctatttgttg tatatggggg
     6721 tgttcctcta tatatagctg agacagcaat agtttatatt gcattgcatg agttttataa
     6781 ggcatttaaa ataaaagatg tacatccaat attcattata ggatatttat tttctattta
     6841 tttagctgta aaaaatattt ttaatttacc attagaatat acttatgcag taatttttat
     6901 tttattttta gcaagtataa tttatatgtt gatgggcaag aacaatgtta tagatgtttc
     6961 gattacgttt ttaggagttt tttatatagg agtattttta gattttataa ttataactat
     7021 aaatggattt gaaaaaggta gcatatatgt atggcttata tttgtgatat cttttatgac
     7081 agatatattt gcgtatttta gtggttattt acttggaaaa cataagctaa ttcctaaagt
     7141 tagtcctaaa aagactatag aaggagctat tggagggata ataggaagta cactttgttg
     7201 tattttattt ggatatttat ttggcattga tttgcttcaa ttagcgataa taggaagtat
     7261 aggaagtgta atagctcaat taggagattt atttgcatct tctataaaga gatatgtagg
     7321 gataaaggat tatggaaaga taatacctgg tcatgggggt atattagata gatttgatag
     7381 tgttatatta gttgcacctt ttgtatatag tgcgattaaa ttttttatta gataaagatt
     7441 ggttggattt aaaagagcaa tctataattg ctagtaatga aaatagctat gtaactttta
     7501 aatgcagaga tattctgcag ttattagtta catggctatt ttcattgttc aaaaatttag
     7561 aagatattgg ggaattgtat aagaatatgt attactttat aataagatac tatgtttgta
     7621 gtaagtatat tttttatact ataactatca ttaaaaaata tttttttgtt taataatttc
     7681 gatattttta gctttatcat ctatt
//
LOCUS       DAEPXF010000176         7545 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain HB27
            SAMN13376389-rid12479423.denovo.183, whole genome shotgun sequence.
ACCESSION   DAEPXF010000176 DAEPXF010000000
VERSION     DAEPXF010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13376389
            Sequence Read Archive: SRR10518432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7545)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7545)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001069624.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001069624.1
            Genome Coverage       :: 315x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/15/2021 01:54:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,165
            CDSs (total)                      :: 4,076
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 89
            rRNAs                             :: 10, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 10 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 19 of 69
            Pseudo Genes (incomplete)         :: 44 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 10 of 69
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7545
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13376389-rid12479423.denovo.183"
                     /strain="HB27"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="China: Hebei"
                     /collection_date="2017"
     gene            158..1351
                     /gene="nifV"
                     /locus_tag="KRL67_003197"
     CDS             158..1351
                     /gene="nifV"
                     /locus_tag="KRL67_003197"
                     /EC_number="2.3.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocitrate synthase"
                     /protein_id="HBH1524043.1"
                     /translation="MCVISKDRAKEIKIVDTTLRDGEQTAGVVFANREKIMIAEMLSD
                     LGVDQIEVGIPTMGGDEKNVIKHICSRNLKSDIMAWNRAVIKDVEESISCGVDAVAIS
                     ISVSDIHIENKLRTSRGWVLENMAKTVEFAKKNGLYVSVNGEDASRADIDFLTEFINV
                     GKQAGADRFRYCDTVGVMNPFSIKNAIETLYERTNFDIEMHTHNDFGMATANALAGIA
                     AGANYVGVTVNGLGERAGNAALEEVLMALKCVYKCDLNNIDTRKFRGICEYVAQASGR
                     ILPTWKPVVGDNMFIHESGIHADGALKDPHNYEPFDPSEVNLERKIVIGKHSGRAAVI
                     NKLSEYEMYISPENATKLLNAIRATSIRLKRSLMDKEILQLYCDILAHEKGTTEEEAV
                     RGSYI"
     gene            1376..3301
                     /locus_tag="KRL67_003198"
     CDS             1376..3301
                     /locus_tag="KRL67_003198"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase"
                     /protein_id="HBH1524044.1"
                     /translation="MGDNIVYKIIKKHIVDGEAVAGSSIGIKIDQTLTQDSTGTMTYL
                     QLEAMGIDKVKTKRSVAFVDHNMLQQGFENADDHKYIQTVADKYGVYFSKPGNGICHQ
                     VFLERFSTPGDTLLGSDSHTPTAGGVGMMAIGAGGLDVALAMAGGAYYIKAPKVCKVN
                     LVGKLNNMVSSKDIILEVLRKQTVKGGVGKVYEYGGEGVKSLSVPQRATITNMGAELG
                     ATTSIFPSDEKTLEFFKSQGREDAWIELKPDADAVYDEEITINLDELKPLAAKPHSPD
                     NVDEVENIGKIKIDQVAIGSCTNSSYEDLMKVAQILKGNKVHKDVSLVIAPGSRQVME
                     MIARNGALADIISAGARILENSCGPCIGMGQSPGTDSVSLRTFNRNFYGRSGTLSAQV
                     YLVSPEVAAVSAIKGVLTDPREFDIKFTNLDVNEFLIDDSMIIKPADVGSDVEVVRGP
                     NIKPFPLNTELSQSIDGKVILKTEDNITTDHIMPSNAKLLPFRSNIPYLANYCFNTVD
                     TEFPQRAKDNNGGFIVGGDNYGQGSSREHAALAPLYLGVKGVIVKSFARIHKANLINS
                     GIIPMEFCDDKDYENISLLDNLEIPNILDNLGSGILEVKNTTKRTSFKVKVELSAKEV
                     DVLKAGGKLNYTKNQAN"
     gene            3323..4318
                     /locus_tag="KRL67_003199"
     CDS             3323..4318
                     /locus_tag="KRL67_003199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate/isopropylmalate dehydrogenase family
                     protein"
                     /protein_id="HBH1524045.1"
                     /translation="MYKVTLIPGDGIGPEVAKAMKKVVEATGVEIEWEEVNAGEAVIE
                     EYGTPLPEYIIDSIKKNKIAIKGPITTPVGKGFRSVNVTLRQALDLYVNLRPIKSFKG
                     IKSRYEDVDLVVVRENTEDLYAGIEHKIGDYAAESIKIITRSASERIIDFACNYVKDN
                     KRKKVTAIHKANIMKMSDGLFLDVFREVASKHGVEYDDLIVDAAAMNLVLNPENYDVM
                     VMPNLYGDILSDLGAGLVGGLGIIPSANIGKDCAIFEAVHGSAPQIAGQNKANPTALI
                     QSSVMMLRYLGEYENAQKIETALEKVFLEGSKLTVDLGGSASTTEFADEVCKYIV"
     gene            4647..5120
                     /locus_tag="KRL67_003200"
     CDS             4647..5120
                     /locus_tag="KRL67_003200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HBH1524046.1"
                     /translation="MVGSMEVCLIKTLDSNILREVGTLSRAVNSINDIKYKELKLQKG
                     QFTFLTRVCENPGINLVELSNMLKVDKATTTKAIQKLIKAGYVDKKQDKFDKRGYNLT
                     PTDKSLEVYELIIEEENRSIEICFDNFTDEEKKVVTKLLEKMSKNVENEWFKVKR"
     gene            5154..5648
                     /locus_tag="KRL67_003201"
     CDS             5154..5648
                     /locus_tag="KRL67_003201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBH1524047.1"
                     /translation="MIRKANMNDLESIMKIIKSTVEEMKIYNNTQWDENYPLEKDFVS
                     DIKKQDLYIYEVDGEVAGFICLNYEEPKEYLGLNWSSNKKAMVIHRMAVNPSFRKTGI
                     ASKLVDFAEKLAVENNVSYLKSDTYSINSKMNLLLIKCGFIKIGEMSFLGKEKSFYCY
                     DKIL"
     gene            5745..6266
                     /locus_tag="KRL67_003202"
     CDS             5745..6266
                     /locus_tag="KRL67_003202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HBH1524048.1"
                     /translation="MIEFLKKRRSIRKYKDVEVEKEKLDKILKAALLAPSSKGLRTWE
                     FIVVDDKEKLINLSQCRTKGGGFFLKNAPLAIVIIADKEKNDVWIEDASIAASYIQLQ
                     AHELGLGSCWIQVRNRMHDDNIEADKYIREELKVPSKYSVECIISIGYPDEEKKAYSD
                     SDLDYEKVHFNNF"
     gene            6455..6766
                     /locus_tag="KRL67_003203"
     CDS             6455..6766
                     /locus_tag="KRL67_003203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HBH1524049.1"
                     /translation="MGIFKTKMDEDWKVNYIKEFNEMRDSYESKLQKKQFEVDSLKSE
                     LDRLRSYKNSLKPKEKQITDDDINNIKSLRRDGLSYKEISNQTSWSKATVSRVLNGLY
                     D"
     gene            6794..7480
                     /locus_tag="KRL67_003204"
     CDS             6794..7480
                     /locus_tag="KRL67_003204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HBH1524050.1"
                     /translation="MKYSYKKCIIDSILLMFIVQILRMILNYVLLSQFEFTLENFNII
                     NLISFTLVGLSLILFLKDNSLYNKVRNRKITEAFEENKNNILIEKCKLILFVVVLSLA
                     IIATYCTKGYVLFNVTMMTLSVLIVPIFEELFFREYIWNYLSNFIKSKSKIICITSIL
                     SGIYNIGYIDIIRNYVILYNNSSYTFEVVISKIMIGTVFGIVLGLVKYRSRDVGFCIL
                     LRSLFAIFIR"
BASE COUNT         3018 a          753 c         1464 g         2310 t
ORIGIN      
        1 aatataatag attacaagat atgaaaagct aaaaaaataa attgcaaatg aaattaaaat
       61 gaataaaaaa taaaaaaata atcataagtt agaggtatca atgaaaaata agacttaata
      121 tttttaaaat taaaagtata ctaagggggg cattaatatg tgcgttatat caaaagatag
      181 agcaaaggaa ataaaaatag tagatactac tttaagagat ggagagcaaa ctgctggggt
      241 tgtatttgca aatagagaaa aaataatgat tgctgaaatg ttaagtgacc taggtgttga
      301 ccaaattgaa gttggtatac ctactatggg tggagatgaa aaaaatgtaa taaagcacat
      361 atgtagtaga aatttaaaat cagatataat ggcttggaat agagcagtaa taaaagatgt
      421 tgaagaatct atttcttgtg gtgttgatgc agtagctata tcaatatcag tatctgacat
      481 tcatatagaa aataaattga gaacttctag aggctgggtt ttagaaaata tggctaaaac
      541 agttgaattt gcaaagaaaa atggattata tgtttcagta aatggagaag atgcttcaag
      601 agcagatata gacttcttaa ctgaatttat aaatgttgga aaacaagcag gagcagatag
      661 atttagatac tgtgatacag taggagttat gaatcctttt tctataaaga atgcaataga
      721 aactctttat gaaagaacta attttgatat agaaatgcat acacataatg actttgggat
      781 ggcaactgca aatgcacttg caggaatagc agcaggagca aattatgttg gtgttacagt
      841 aaatggatta ggtgaaagag caggaaatgc agcattagaa gaagttttaa tggcacttaa
      901 gtgtgtttat aaatgtgatt taaataatat agatactaga aaatttagag gaatatgtga
      961 gtatgtagca caggcatcag gaagaatcct tcctacatgg aaaccagtag ttggggataa
     1021 tatgtttata catgaatctg gtatacatgc agatggagct ttaaaagacc cacataacta
     1081 tgaaccattt gacccaagtg aagtaaatct tgaaagaaaa atagtaatag gtaaacattc
     1141 aggaagagct gcagttataa ataagttgag tgaatatgaa atgtatattt ctccagaaaa
     1201 cgctaccaaa ctattaaatg ctataagagc tacatctata agattaaaaa gaagtttaat
     1261 ggataaagaa attcttcaat tatattgtga tatactagca catgagaaag gcacaacaga
     1321 agaagaagcg gttagaggtt catatattta agatataatt gtttaggagg aaaatatggg
     1381 agataatata gtttataaaa taattaaaaa acatatagta gatggagaag ctgttgctgg
     1441 aagttcaatt ggtataaaaa tagaccagac tttaacacaa gattctactg gaacaatgac
     1501 atatcttcaa ctagaagcta tgggaataga taaagtaaag actaaaagaa gtgtggcatt
     1561 tgtagaccat aatatgttac aacaaggatt tgaaaatgca gacgaccata agtatataca
     1621 aacagtggca gataaatatg gagtttattt ctctaagcca ggaaatggta tttgtcatca
     1681 agtattttta gaaagatttt caacgccagg agatacactt ttaggttctg atagccacac
     1741 acctactgct ggaggtgtgg gcatgatggc tattggagct ggtgggttag atgttgcttt
     1801 agccatggca ggaggagcat actatataaa agcaccaaaa gtctgtaagg tgaatctagt
     1861 tggaaaatta aataatatgg tatcatcaaa agatataata ttagaagtgt taagaaaaca
     1921 aactgtaaaa ggtggagtag gtaaagttta tgaatatggt ggagaaggag tcaaaagcct
     1981 atctgttcca caaagagcta caataacaaa tatgggggct gaacttggag caactacttc
     2041 aatcttccca agtgatgaaa aaacactaga attttttaag agtcaaggaa gagaagacgc
     2101 atggatagag ttaaaaccag atgcagatgc agtttatgat gaagagatta ctataaactt
     2161 ggatgaatta aaaccattag ctgcaaaacc tcatagccca gacaatgtag atgaagttga
     2221 aaatataggc aagattaaaa tagaccaagt ggcaatagga agttgtacaa attcttctta
     2281 tgaagattta atgaaagtag cacaaatcct aaaaggaaat aaagtacata aggatgtgag
     2341 cttggtaata gcaccaggtt caagacaagt tatggagatg attgctagaa atggggcttt
     2401 ggcagatata ataagtgctg gagctagaat actagaaaat tcttgtggtc catgtattgg
     2461 tatgggtcaa tccccaggta ctgactctgt gtctttaaga actttcaaca gaaattttta
     2521 tggtagaagt gggactttgt cagcacaagt atatttagta agtccagaag tggcagcagt
     2581 aagtgcaatc aagggagtac ttacagaccc aagagaattt gatattaaat ttacaaattt
     2641 agatgtaaat gaatttttga tagatgacag catgataatt aaacctgctg atgtaggaag
     2701 tgatgtagaa gtagttagag gacctaatat aaaacctttc cctcttaata cagagctaag
     2761 tcagtctata gatggaaaag ttatattaaa gacagaagat aatataacta ctgaccatat
     2821 aatgccatca aatgctaagt tacttccttt tagaagtaat ataccgtatc ttgcaaacta
     2881 ttgtttcaac actgtagata cagaatttcc tcagagagca aaagacaata atggtggatt
     2941 tatagttggt ggagataatt acggtcaagg ttcaagtaga gaacatgcag ctttagcacc
     3001 attatatcta ggtgttaaag gggtaatagt taagagcttt gcaagaatcc acaaggctaa
     3061 cttgataaat agtggtataa ttccaatgga gttttgtgat gataaagatt atgaaaatat
     3121 atctctttta gataatctgg agataccaaa tattttagac aatctaggta gtggaatttt
     3181 agaagttaag aatacaacta aaagaacttc ttttaaggtt aaagtagaat tatcagcaaa
     3241 agaagttgat gtattaaaag ctggtggaaa attaaactac actaaaaatc aagctaatta
     3301 atatgaaaag agggaatatt gaatgtataa agtgacactt ataccaggag atggaatagg
     3361 accagaagtt gcgaaagcta tgaaaaaggt agtagaagct actggagtag aaatagaatg
     3421 ggaagaagtt aatgcaggag aggcagttat tgaagagtat ggtactccac ttcctgaata
     3481 tattatagat agtataaaaa agaacaaaat agctataaaa ggcccaatta ctactcctgt
     3541 aggtaaagga tttagaagtg taaatgtaac tttaagacag gcattagatt tatatgtcaa
     3601 tttaagacct attaaaagtt ttaaggggat aaaatcaaga tatgaagatg ttgatttagt
     3661 ggttgtaaga gaaaatacag aagatttata tgctggaata gaacataaaa taggtgatta
     3721 tgcagctgaa agtataaaga taataacaag aagtgcaagt gaaagaatca ttgattttgc
     3781 atgtaactat gttaaagata ataagagaaa aaaagttaca gctatacata aggcaaatat
     3841 aatgaaaatg tctgatggat tgtttttaga tgtgtttaga gaagttgcat caaaacatgg
     3901 tgtagaatat gatgatttga ttgtagatgc agcagctatg aacttagttt taaatccaga
     3961 aaattatgat gtaatggtaa tgccaaatct ttatggagat atactatcag atttaggtgc
     4021 tgggcttgtt ggaggtttag gtataattcc aagtgcaaat attggtaaag attgtgcgat
     4081 atttgaagct gttcatggaa gcgctccaca aatagcaggt caaaataagg ctaatccaac
     4141 agcacttatt caatcatcag taatgatgct tagatactta ggcgaatatg aaaatgcaca
     4201 aaaaatagaa acagctttag aaaaagtatt tcttgaagga agtaaattga ctgtagattt
     4261 aggtggctca gcttctacta cagagtttgc ggatgaggtg tgtaagtata ttgtatagat
     4321 tttatttagg gaaaaagtaa aatatagatt aaaggaaaga ggctttctca aaatagagat
     4381 aagccttttt ctttattgaa atgataaaat atatttgatt ttatacaaaa gagagttcct
     4441 cataaacgaa aaagttaatt ttgggatatc cattttaatt ttaaaattaa ttgattaatg
     4501 tgcaaagaaa gattaaacaa atttattatt tttaattgaa ctttcaacca aaatatggta
     4561 aaatttaaaa aagttgaact ttcaactaaa aataaatata ttgattatgt gaaattctat
     4621 aaaacaggaa ctctttgact ttagtcatgg taggttcaat ggaggtgtgt ttgattaaga
     4681 ctttagatag taatatatta agagaagtag gtactttatc tagagcagta aattcaataa
     4741 atgatattaa gtacaaggaa ttaaagttac aaaaaggaca atttacattt ttgacaagag
     4801 tctgtgaaaa tcctggtata aatcttgtgg aattatcaaa tatgttaaag gtggacaagg
     4861 ctacaacaac caaggctata caaaaattaa taaaggcagg atatgtagat aaaaagcagg
     4921 ataagtttga taaaagagga tataatttaa ctccaactga taaatctttg gaagtttatg
     4981 agttgataat tgaagaagaa aatagaagta tagagatatg ttttgataat tttacagatg
     5041 aagaaaaaaa agtggtaact aagttattag aaaaaatgag caaaaacgta gaaaatgaat
     5101 ggtttaaagt aaagagatag taaataaata cattaactta aggagctatt tttatgatta
     5161 gaaaagcgaa tatgaatgat ttagaaagca ttatgaaaat tataaaatct acagtggaag
     5221 aaatgaagat ttataataat acacaatggg atgaaaacta tccattagaa aaagactttg
     5281 tttcagatat taagaaacaa gatttatata tttatgaagt ggatggagag gtagcagggt
     5341 ttatatgtct taattatgaa gagccaaaag aatatttagg tttaaattgg tcatctaata
     5401 aaaaagccat ggtaatacat agaatggctg taaatccaag ctttagaaag acaggaatag
     5461 catctaagtt agttgatttt gctgaaaaat tagctgtaga aaataatgta tcatacttaa
     5521 agagtgatac atattcaata aactcaaaaa tgaatttatt acttataaag tgtggtttta
     5581 taaaaatcgg agaaatgagt tttttaggaa aggagaaatc tttctactgt tatgataaaa
     5641 tattataaaa agcatttata atacaagggg taaataatag attttcagca ttagtaaggg
     5701 cataatataa cctagtgtat gctattatga aagggatgat ttatatgatt gaatttctaa
     5761 agaaaagaag aagtattaga aaatacaagg atgttgaagt agaaaaagaa aaattagata
     5821 aaatattaaa agctgctctt ttagctccat catcaaaggg gcttagaaca tgggaattta
     5881 tagtggtaga tgataaagaa aagctaatta atttatctca atgtagaact aaaggtggtg
     5941 gatttttctt aaaaaatgca cctttagcta ttgtaattat tgcagataaa gaaaaaaatg
     6001 atgtttggat agaggatgca agtatagctg cttcatatat acaacttcaa gctcatgaac
     6061 taggtttagg ttcttgttgg atacaagtaa gaaatagaat gcatgatgat aatatagaag
     6121 ctgataagta cattagagaa gaattaaaag tacctagcaa gtacagtgta gaatgtataa
     6181 tttctatagg atatcctgat gaagaaaaga aagcatacag tgactctgat ttagactatg
     6241 agaaggttca ttttaacaac ttttagtata taatatgctg tattaagaca ttatattttt
     6301 ttctattgtt aatatatact taaaattgta aaaatatatc tgaattttac taacttatct
     6361 aggggttatg tacaaggatt ttaaagataa atatagaaat taatgtttat aatcgataca
     6421 taactttagt aacaaaattt aaggaggtta aagtatgggt atatttaaaa caaaaatgga
     6481 tgaggactgg aaagtaaact acattaaaga gtttaatgaa atgagagatt cttatgagag
     6541 taaacttcaa aaaaaacagt ttgaggtaga tagtttgaaa tcagaattgg atagactaag
     6601 aagctataaa aatagcctta agccaaaaga aaaacaaatt actgatgatg atataaataa
     6661 tataaagagt ttaagaaggg atggcttaag ctataaagaa atttcaaatc aaacaagttg
     6721 gagcaaagca actgtaagta gagtattaaa tggactttac gattagcata tacatggggt
     6781 gatttaaatt gggatgaagt atagttacaa aaaatgcata atagattcta ttttattgat
     6841 gtttatagtt caaatattaa ggatgatttt gaattatgta cttctaagtc aatttgaatt
     6901 tactcttgaa aattttaata ttataaactt aatttctttt acattagttg gtttatcatt
     6961 aattttattc ttaaaagata attctttgta taacaaggtt agaaatagaa aaatcacaga
     7021 agcttttgaa gaaaataaaa acaatatact tattgaaaag tgtaagctga tactttttgt
     7081 tgtggtttta tctttagcaa taattgcaac ttattgtact aagggatatg tactttttaa
     7141 tgtaactatg atgactttgt ctgtattgat agttccaata tttgaagaat tattttttag
     7201 agaatatata tggaactatc taagtaattt tataaagtca aagagtaaaa ttatttgtat
     7261 aacatctata ctatctggaa tttataatat aggatatata gatattataa gaaattatgt
     7321 aatattgtat aacaattcaa gctacacctt tgaagttgtt atttcaaaaa ttatgatagg
     7381 cactgttttt ggtattgtac ttggattagt taagtatagg tctagagatg taggattttg
     7441 tatactattg aggagtttat ttgctatatt tattaggtag atagtataat tagtttataa
     7501 ggttgtttta aatttaattt taaatcaacc ttattttatt tgtgt
//
LOCUS       DAEPXF010000177         7473 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain HB27
            SAMN13376389-rid12479423.denovo.184, whole genome shotgun sequence.
ACCESSION   DAEPXF010000177 DAEPXF010000000
VERSION     DAEPXF010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13376389
            Sequence Read Archive: SRR10518432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7473)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7473)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001069624.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001069624.1
            Genome Coverage       :: 315x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/15/2021 01:54:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,165
            CDSs (total)                      :: 4,076
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 89
            rRNAs                             :: 10, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 10 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 19 of 69
            Pseudo Genes (incomplete)         :: 44 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 10 of 69
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7473
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13376389-rid12479423.denovo.184"
                     /strain="HB27"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="China: Hebei"
                     /collection_date="2017"
     gene            complement(175..570)
                     /locus_tag="KRL67_003205"
     CDS             complement(175..570)
                     /locus_tag="KRL67_003205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HBH1524051.1"
                     /translation="MKALEFLNKAGVYYLATVDENNQAHVRPLGFVMEYNGKLTFCTS
                     NQKDMFKQLIFNPKVELCCIDQNLNTLRILGEAVFVTSKETQAKALEIMPNLGQMYSV
                     GDGKFEIFSIDKAKVSCCSMSGKKIDVEL"
     gene            711..1103
                     /locus_tag="KRL67_003206"
     CDS             711..1103
                     /locus_tag="KRL67_003206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBH1524052.1"
                     /translation="MSINTEKNITKNVEELTCPIRYALDIVGGKWKLPIICMLAVENP
                     IRYSSIKRKLDGITNTMLAQSLKDLESNGIVHRKQYNEIPPKVEYTLTSKGKSIVPIL
                     QQFANWGATNMQEKSTCGLSCKECRKIK"
     gene            complement(1204..1620)
                     /locus_tag="KRL67_003207"
     CDS             complement(1204..1620)
                     /locus_tag="KRL67_003207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3788 domain-containing protein"
                     /protein_id="HBH1524053.1"
                     /translation="MNWQDAFFQDTKPTFEQINEFINNPLWDKLNKTLVSTYNVEPKL
                     EYSKCSMQRGWNVKYKKRGKSLCTLYPQEGYFKALVIVNESNRIEVDLLINTCCDYIK
                     KIYNEVNFFNGSKWLMIQVDSLLVLNDMLELIKFRA"
     gene            complement(1635..2546)
                     /locus_tag="KRL67_003208"
     CDS             complement(1635..2546)
                     /locus_tag="KRL67_003208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HBH1524054.1"
                     /translation="MHAWESIQITLDLIEANLSEEISINELANKANLSPFYYQRLFKR
                     LVNKTVMEYIKLRRLARASEYLAEHKNRILDVALNFGFASHETFTRSFKKAYGLTPEK
                     YRANPVKLNHFIKPELILNYAMVDEGVPLIADDIVIEVNRKTLSNPRTFVGIEIEVPI
                     CQLVGGETTGIAIVEELWMKLGSQRHNIPNQIPGGNEFAALYMGNAKEGNCMYMAGVE
                     VESGTSVEGYSTFELPAKEYLVCGFEAENFNELVNSAVFKADKFMERWMKKHNLTTAD
                     FAIEMYYPTTPEVAYLEHWIVPVPIEQ"
     gene            complement(2726..3748)
                     /gene="splB"
                     /locus_tag="KRL67_003209"
     CDS             complement(2726..3748)
                     /gene="splB"
                     /locus_tag="KRL67_003209"
                     /EC_number="4.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore photoproduct lyase"
                     /protein_id="HBH1524055.1"
                     /translation="MFNPKRIIFEKGTLDTDVGKNIYNKFRDNKDVEIINISSNRIKE
                     HIPGENLYEQYRSGKQTMVVGIRKSLKFQTCKPSANYQLPLVSGCMGRCEYCYLNTQL
                     GDKPFIRVFVNVDEILEQTKKYIEDRKPQVTIFEGAATSDPVPVEPYTNSLKKTIEFF
                     GREEKGRFRFVTKYSDVDSLLDAEHNNHTEIRFSINTPRIINEHEHYTASANKRIESA
                     VKLASAGYQIGFIIAPVFLYEGWKEEYKNLIISIKEALPKDFDKKIIFEVISHRYTTK
                     AKNRILEIYPDTLLPMENEDRKFKYGQFGYGKYVYDKEQLDDMKEFFLKEINGVFKNS
                     LIKYII"
     gene            complement(3975..5105)
                     /locus_tag="KRL67_003210"
     CDS             complement(3975..5105)
                     /locus_tag="KRL67_003210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HBH1524056.1"
                     /translation="MNFNYNLPVNILFGRGRINDVGKEVSKYGKKVLIVTGKNSTKKT
                     GLLDKTIDLLKDSKIEYEVFDRVEQNPLITTIYSGVEIIKATGCDCVLGLGGGSIMDA
                     AKSIAFSYKNPGDLNDYIFGIRQGEEALPIILVPTTSGTGSEGNCFAVLTNSETKDKK
                     SIKKNSMYAKASIIDPELMVTMPKHIIASVGFDALAHNMEAYLSNGRNPLADVQAIYG
                     IELISENLIKVYNDVNDLEAWEKITLASTIGGMTIGTAGTALPHGMEHPVSGLKDVMH
                     GKGLAALTPTIIESSWDSDIERFGNISKVLGGTCAEDCSYAIRNFLKKIDLDIKLSDL
                     GIKKSDIDWLTDNCMKISAANIKRHPKYFNKEQIKEMYHKSL"
     gene            complement(5493..7430)
                     /gene="htpG"
                     /locus_tag="KRL67_003211"
     CDS             complement(5493..7430)
                     /gene="htpG"
                     /locus_tag="KRL67_003211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HBH1524057.1"
                     /translation="MEFEKGSISIHTENIFPIIKKWLYSDKDIFIRELISNGCDAVSK
                     HKRLVSLGEISENKSSDYKITVSVNKGEGTLKFIDNGIGMTEEEIKKYINQVAFSGAE
                     DFFNKYKDKMEESNDIIGHFGLGFYSAFMVSKKVQIDTLSYTEGATPVRWISEGGTEY
                     EISKSDTRNDRGTTITLFIDDDSKEFLDEFTVRGIINKYCSFLPVEIYLEDVERLERE
                     AKEAEEKAKKQKEDGKEEVVDAKVIEPLNDTNPLWLKSPKDCTDEEYKEFYRKVFHVF
                     DEPLFWIHLNVDYPFNLKGILYFPKLKNEFELTEGKVKLYNNQVFVADNIKEVIPEFL
                     LLLKGVIDCPDLPLNVSRSFLQNDRDVSKISKHIIKKVADKLKSLCKNEREEYNKFWD
                     DIQIFIKYGCLKDESFYEKVKECILFKTIDDEYITLQDYLEKCKDKHENKVFYVSDKE
                     QQSQYIKLFKEYDLSAVVLNSSIDTHFISFMEYKENGVKFNRIDADLSDVLKDKNENK
                     DSEENKEEIAKIEGLFKEAVGERVKNYSVEGLKNEDTPAMVLVSEQSIRMAEMQSRFA
                     GMDLGMNFEEEKTLVINENSPIIKKLVSLKDDEEKKDKITLICNQIADLALLSNKELK
                     PDELDSFVQRSNKLMSLLIEL"
BASE COUNT         2423 a         1296 c          789 g         2965 t
ORIGIN      
        1 tctagtatgt aattattcaa aatagtatat cattattgaa atacacatct aataactaaa
       61 aatttttaca tattcattat ttaaaaatct ttttattgta tttaaattaa atctatcaat
      121 atttcagtaa taatgttttc ttaactttca ctatggatgt cgattcagtt ataattaaag
      181 ctctacatca atctttttac cactcattga acaacaacta acttttgcct tatcaataga
      241 gaatatttca aacttaccat ctcctacaga gtacatctga cctaaatttg gcattatttc
      301 aagtgcctta gcttgtgttt cttttgatgt aacaaatact gcttcaccta aaatacgtag
      361 agtattaagg ttttggtcta tacaacaaag ttcaaccttt ggattaaaaa taagttgctt
      421 aaacatatcc ttttgattag atgtacaaaa tgtaagttta ccattgtatt ccataacaaa
      481 tcctaaagga cgcacatgtg cttgattgtt ctcatcaact gttgctaaat aataaactcc
      541 tgccttgttc aaaaattcta atgctttcat aattcttaca ccccttttaa atacttgtat
      601 taaggattat cctttgatat aattttaata taaaatacag aaaaaacaag tatgcagttt
      661 tttctgataa agtataaaaa agatactact ataactaagg agtaattaaa atgagtataa
      721 acactgaaaa aaatataact aaaaacgttg aagaattaac ctgtccaatc cgttatgcat
      781 tagacattgt cggtggtaag tggaaattac ctattatatg tatgttagca gttgaaaatc
      841 caattagata tagtagcata aaaagaaaat tagatggcat tacaaatact atgctagctc
      901 aatcattaaa agatttagag tctaatggta ttgttcatcg taaacaatat aatgaaattc
      961 ctccaaaagt tgaatataca ttaacaagta aaggtaaaag tattgtgcct attttacaac
     1021 aatttgctaa ttggggtgct actaatatgc aagaaaaaag cacatgtgga cttagttgca
     1081 aagagtgtag aaagataaag taaatttaaa ttttactaaa ttattcgtat tatagcctat
     1141 gtattaattt atctgattga atataaaaac aggatgactt atatatccat cctgttctat
     1201 atgttaagct ctaaatttaa ttagttcaag catatcgttt aataccaata aactatctac
     1261 ttgaatcatt agccatttac ttccattaaa aaaatttacc tcattatata tttttttgat
     1321 ataatcacaa caagtattaa taagtaaatc aacctctatt ctattgcttt catttacaat
     1381 aaccaaagct ttaaaataac cttcttgtgg atataaagta catagtgatt tacctctttt
     1441 tttgtatttt acattccagc ctctttgcat tgaacattta ctgtattcaa gcttaggttc
     1501 tacattataa gtagaaacca aagttttatt taacttgtcc cataatggat tattaataaa
     1561 ttcattaatt tgctcaaatg taggtttagt atcttgaaaa aatgcatctt gccaattcat
     1621 attcatcatc ctttctattg ttcaatagga accggaacaa tccaatgttc cagatatgct
     1681 acttcaggtg tagtggggta atacatctca attgcaaaat cagcagttgt taaattatgc
     1741 ttcttcatcc atctttccat aaatttatcc gctttaaaaa cagctgaatt aactaactca
     1801 ttaaagtttt ctgcctcaaa tccacatact aaatattctt tagctggtaa ctcaaaagta
     1861 ctatatcctt ctactgatgt acctgactct acctctacac ctgccatata catacaattt
     1921 ccttcttttg catttcccat ataaagagca gcaaattcat ttccacctgg aatttgattt
     1981 ggtatattat gtctttgact tccaagtttc atccataatt cctctactat tgcaataccc
     2041 gttgtttcac cacccaccaa ctggcaaata ggtacttcta tttctattcc aacaaacgtc
     2101 cttggattac tcaatgtctt acgattgact tcaatcacta tatcatctgc aatcagtgga
     2161 acaccttcat caaccatagc ataatttaaa attaattctg gcttaataaa gtgattgagt
     2221 tttactggat ttgctctata tttttctgga gttaatccat atgctttttt aaaggaacgt
     2281 gtaaatgtct catgactagc aaatccaaaa tttaaagcta catcaagtat acgattctta
     2341 tgttctgcta agtattcaga agctcttgca agacgacgaa gctttatata ctccattaca
     2401 gtcttattta ccaatctttt aaatagtctt tgatagtaaa atggtgataa atttgcttta
     2461 tttgccaatt cattaattga tatttcttcc gacaaatttg cttctattaa atccaatgta
     2521 atttgaatag actcccatgc atgcatatta taactcctcc tgatattttt gttactctca
     2581 tcctttatag tatcaaaact agaaaaaatg ttcttgatag atagtgccct tttatattct
     2641 tatatgataa tactaaatct catagcttta ttataaacat aaaatatata aaaataaaat
     2701 atagcaaatc tttaggtaag atttgctata ttatatattt tattaaacta tttttaaata
     2761 ctccatttat ttccttaaga aagaactctt tcatatcatc taattgttct ttatcataca
     2821 cgtacttacc atatccaaat tgaccgtatt taaactttct atcctcattt tccattggca
     2881 atagtgtatc tggataaatc tctagtattc tattttttgc tttagttgta tatctatgag
     2941 atataacttc aaatataatt tttttatcaa aatcctttgg taaagcttct tttatactta
     3001 taatcagatt tttatactct tctttccaac cttcatataa aaacacaggt gctattatga
     3061 aaccaatctg atagccagcg ctagcaagtt ttacagctga ctctattctt ttatttgcag
     3121 atgctgtata atgctcatgt tcatttataa ttctaggagt attgatacta aatcgtattt
     3181 ctgtatgatt attgtgttca gcatccaata agctatccac atcactgtat ttagtaacaa
     3241 atctaaatct tcctttttcc tctcttccaa aaaactctat tgttttcttc aaagaattag
     3301 tatatggctc tactggaact gggtctgatg tagccgcccc ttcaaatatg gttacttgtg
     3361 gctttctatc ttcaatatat ttttttgtct gttcaagtat ttcatctaca ttcacaaata
     3421 cccttataaa tggtttatca cctaattgtg tattcaaata acaatactca catcttccca
     3481 tacatccact gacaagtggt aattgataat tagcagaagg tttacatgtt tgaaacttca
     3541 gactttttct aattccaact accatagtct gcttcccact tctatattgt tcatataaat
     3601 tttcacctgg aatatgttct ttaattctat ttgatgatat attaattatc tctacatctt
     3661 tattatctct aaatttattg tatatatttt tacccacatc agtgtctaaa gttccttttt
     3721 cgaatattat tcttttggga ttaaacattt atttcacctc tacttaaagt ttttaaatcg
     3781 aagactttta taaaatatta cttaattaat cttccctttt ttaaactcct ttatttatta
     3841 gaaagtaaaa taaaagctta agataagttt attgtatatc taacttctat acacaatttt
     3901 tttgctatta attacttata attttttatt attaatttat aatttttata taaaactaaa
     3961 tatgtaaagt caaattatag agacttatgg tacatttcct ttatttgttc cttattaaaa
     4021 tactttggat gtctcttaat atttgcagct gatattttca tacaattatc agttaaccaa
     4081 tctatatctg attttttaat acctaaatca cttagtttta tatctaaatc tattttttta
     4141 aggaaatttc taattgcata tgaacaatct tctgcacaag ttcctcctag tacctttgag
     4201 atattaccaa atctttcaat atcactatcc catgaacttt ctataatagt aggagtcaaa
     4261 gctgcaagtc cttttccatg cattacatct ttaagaccac ttacaggatg ctccattcca
     4321 tgtggcaagg ctgtgcccgc tgtaccaata gtcatccctc caatagtact tgcaagtgtt
     4381 attttttccc atgcttctaa atcattaaca tcattatata cctttattag gttttctgat
     4441 ataagttcaa tcccataaat agcttgtaca tctgcaagag ggtttcttcc attagaaaga
     4501 tatgcttcca tattatgagc aagtgcatca aatccaactg atgcaattat atgctttggc
     4561 attgtaacca taagttctgg gtcaattatt gatgcttttg catacatact attcttcttt
     4621 atagattttt tatctttagt ttctgaattt gtaagtactg caaaacaatt tccttcacta
     4681 cctgtacctg atgttgttgg aactaagatt ataggtaaag cttcttcacc ttgtctaatt
     4741 ccaaatatat aatcatttaa atctcctggg tttttataag aaaaagctat acttttggca
     4801 gcatccataa tgcttccacc accaagccct aaaacacaat cacatccagt tgcttttata
     4861 atttctactc ctgaatatat agtagttata agaggatttt gttctactct atcaaaaact
     4921 tcatattcta ttttagagtc ttttagtaaa tctattgttt tatcaagtaa tccagttttc
     4981 tttgtactat ttttacctgt tactatcaat acttttttgc catatttaga tacttctttt
     5041 cccacatcat taattctgcc tctcccaaac aagatattta ctggcaaatt ataattaaaa
     5101 ttcatgctaa aacctcctat ttctacttta gtaataattt tatattttta acaaagtgta
     5161 attatgtatt taaacataaa ctaaaaaaca atcttttact taattatatg tattatctta
     5221 ttatatatca agttaactat atttaactaa atagagtttt ttattagaac ctacttaaat
     5281 atttaaaaat taaccaaaga taaaaaaata aagaatggtc tgtctcaaaa tagagataaa
     5341 tatatttttc attgttgaaa tgattaaaat aaatttaatt tcacataaaa aaagcgcctc
     5401 atgaactgaa aagttcattt tgagacactt ctttattatc attgatattg aatacttttt
     5461 ataaatttgc ttcaatgtat tcaaattcaa ccttataatt ctatcaaaag actcataagc
     5521 ttattacttc tttgtacaaa tgaatctaac tcatctggct ttaattcttt attagaaagt
     5581 aaagctaagt ctgctatctg gttacaaata agcgtaatct tatctttctt ttcctcgtca
     5641 tctttaagag aaactaattt tttgattatt ggactgtttt catttatcac aagagtcttt
     5701 tcttcctcaa aattcattcc taaatccatt ccagcaaatc ttgactgcat ttctgccatt
     5761 ctaatagatt gttctgatac taacaccata gctggagtgt cttcattttt caatccttct
     5821 acagaataat tcttcactct ttctccgaca gcttctttaa acagaccttc tatcttagct
     5881 atctcttctt tgttttcttc actatcttta ttttcatttt tatcttttaa tacatcagaa
     5941 aggtctgcat ctattctatt aaacttaact ccattctctt tatattccat aaatgaaata
     6001 aaatgagtat ctattgatga gtttaaaaca acagcactta agtcatattc tttaaataat
     6061 ttaatatatt gagattgctg ttccttatca cttacataaa ataccttatt ttcatgtttg
     6121 tccttacatt tttctaaata atcttgtaat gttatatatt catcatcaat agttttaaat
     6181 aatatacatt cttttacctt ctcatagaaa ctttcatctt ttaagcaacc atatttaata
     6241 aatatttgta tatcatccca aaacttatta tattcctctc tttcattttt acaaagagat
     6301 tttaacttat cagctacttt tttaattata tgtttagaaa ttttgcttac gtctctgtca
     6361 ttttgtaaga aacttcttga tacattcaat ggtaagtctg ggcagtctat aacacctttt
     6421 aataaaagta aaaattctgg tataacttct tttatattgt cagccacaaa cacttgattg
     6481 ttgtatagtt ttacttttcc ttctgttaat tcaaactcat tttttaattt agggaagtaa
     6541 agtataccct ttaagttgaa aggataatct acatttaaat gtatccagaa taaaggttca
     6601 tcaaatacat ggaatacctt tctataaaac tctttatact cctcatcagt acagtctttt
     6661 ggagatttta accatagagg attagtatcg tttaaaggct caattacttt tgcatctaca
     6721 acctcttctt ttccatcttc cttttgtttt ttagcttttt cttctgcttc tttagcttct
     6781 ctttctaatc tttcaacatc ttctaaatat atttctactg gtaagaaaga acagtattta
     6841 tttattattc ctcttacagt aaattcatct aaaaattctt tactatcatc atcaataaat
     6901 agtgttatag tagttcctct atcatttcta gtatctgatt tagatatttc gtattctgta
     6961 ccaccttcag atatccatct tacaggagtc gctccttctg tatatgaaag tgtatctatt
     7021 tgtacctttt tagacaccat aaatgcagaa taaaatccta atccaaaatg acctattatg
     7081 tcatttgact cttccatctt atccttatat ttattaaaga aatcttcagc acctgaaaaa
     7141 gcaacttgat ttatgtattt ttttatttct tcttcagtca taccaatccc attgtcaata
     7201 aatttaagtg taccttctcc tttatttaca gatactgtaa ttttataatc tgatgactta
     7261 ttttctgata tttctcctag tgatacaagt cttttatgtt tacttactgc atcacatcca
     7321 ttacttataa gctctcttat aaatatgtct ttatccgagt ataaccattt tttaattata
     7381 ggaaaaatat tttctgtatg aattgaaata ctcccttttt caaattccat tttttatccc
     7441 tcctcatttt ataattaata ttttaaattt ttt
//
LOCUS       DAEPXF010000178         7434 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain HB27
            SAMN13376389-rid12479423.denovo.185, whole genome shotgun sequence.
ACCESSION   DAEPXF010000178 DAEPXF010000000
VERSION     DAEPXF010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13376389
            Sequence Read Archive: SRR10518432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7434)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7434)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001069624.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001069624.1
            Genome Coverage       :: 315x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/15/2021 01:54:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,165
            CDSs (total)                      :: 4,076
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 89
            rRNAs                             :: 10, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 10 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 19 of 69
            Pseudo Genes (incomplete)         :: 44 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 10 of 69
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7434
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13376389-rid12479423.denovo.185"
                     /strain="HB27"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="China: Hebei"
                     /collection_date="2017"
     gene            55..1032
                     /locus_tag="KRL67_003212"
     CDS             55..1032
                     /locus_tag="KRL67_003212"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HBH1524058.1"
                     /translation="MRDKIIEIILNNKSEFISGEELSKQLGVSRAAIWKHMKALKEEG
                     YNIESVNKKGYRLAENPTDLLSPQNIYYKLDTEFIGKNIEHLETIDSTNEHAKRTAIN
                     SVDGTVILSEHQDMGKGRLGRRWDSKPHEGIWMSIILKPDMEPFKAPFITLIAGASVT
                     KALNNLGANTLIKWPNDVILNGKKICGILTELSAEIERTNYIVLGIGINVKIMDFEQE
                     IADKATSLYKEGYMLPRVDIVRNVLTEFEKLYLDYTVNNSKEKTLKICRDYSAIIDKN
                     IYVLKGDNKELVKCLDINEEGNLLVQRSDGSIKEIISGEVSIRGEKGYV"
     gene            1025..1993
                     /locus_tag="KRL67_003213"
     CDS             1025..1993
                     /locus_tag="KRL67_003213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P1 family peptidase"
                     /protein_id="HBH1524059.1"
                     /translation="MYNNILDIEGLKVGQVEDRNGLTGCTVVICEEGATCGVDVRGSA
                     PGTRETDLLDPVNMVQKVHAVVLAGGSAYGLESTCGVSRYLEEKGIGFDVGVGKVPIV
                     VGAVLFDLGVGSFKCRPDLKMGYRACEVANTDILNQGNYGAGCGATVGKIRGQEYAMK
                     GGIGSYSIKLDNGLVVSALIAVNALGDVYENGKVIAGALNDKKTETIDTYKLMKQGES
                     KGGFSVDNTTIGIIATNAKLTKAGCKKISQMGHDGYAKAIFPIHTPHDGDTIFTISTG
                     EIEADITLVGSLAVEVVEKSIINAVKKADSVENIMAYKDIIKKREI"
     gene            2176..2766
                     /locus_tag="KRL67_003214"
     CDS             2176..2766
                     /locus_tag="KRL67_003214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HBH1524060.1"
                     /translation="MMNTSASVRKVNVRKMTIIGVLSAISIMLSMTPLGFIPVGPTKA
                     TIMHIPVIIGAIMEGPIVGAAIGFIFGISSLLNAITNPTVTSFVFINPLVSILPRVMI
                     GVLAYYVYQLIIKATNKVYMSGLITGAIGSLLNTAGVLGMIYVLYADKYLHAMGKSGS
                     AGKVIMALAAANGVPEAIVGALVVAAVATVLKKSKK"
     gene            2785..3555
                     /locus_tag="KRL67_003215"
     CDS             2785..3555
                     /locus_tag="KRL67_003215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HBH1524061.1"
                     /translation="MLLVFDVGNTNMVLGIYKGDKLVNYWRIKTDREKTSDEYGILIS
                     NLFDYDNVNISDIDDVIISSVVPNVMHSLENFCIKYCKKQPLIVGPGIKTGLNIKYDN
                     PKQVGADRIVNAVAGIEKYGAPSILVDFGTATTFCAISEKGEYLGGTIAPGIKISSEA
                     LFQSASKLPRVELAKPGMTICKSTVSAMQSGIIYGYVGLVDKIISMMKKELNCDDVKV
                     IATGGLAKLIASETKSIDYVDGFLTLEGLRIIYEKNQE"
     gene            3692..4660
                     /gene="dusB"
                     /locus_tag="KRL67_003216"
     CDS             3692..4660
                     /gene="dusB"
                     /locus_tag="KRL67_003216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HBH1524062.1"
                     /translation="MKIGNLKLKNKVFLSPMAGVTDLPFRLICKEQGCGLLYTEMING
                     KALCYDDENTKKMLKIEEEEHPVAVQIFGSEPEFMGRAAEIMNDYSNEILDINMGCPA
                     PKVVKNGDGSALMKNPKLAEEVLRAVVKNSKKPVTLKIRKGWDDNSVNAVEIAKIAED
                     CGISALAIHGRTREQFYSGKADWDIIAEIKKNLSIPVIGNGDVFTIEDSINMLDKTGC
                     DAIMIGRGAQGNPWIFKRINHYMNTGEILPEPTLNEKISTAIKHLKLAVEEHGEYVAV
                     REMRKHIAWYLKGLRNSAKLRDEINKIEDYQEVVSKLEYYMEDSLT"
     gene            4769..5248
                     /gene="greA"
                     /locus_tag="KRL67_003217"
     CDS             4769..5248
                     /gene="greA"
                     /locus_tag="KRL67_003217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003514606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HBH1524063.1"
                     /translation="MEENKEFLLTQEGYDKLEEELENLKVVKRKEVAERIKVAISFGD
                     LSENAEYDEAKKEQAQVEERILKLENMVRKAVIIDESKIDLNVVTIGSIVKVKDLEFD
                     EEVEYTIVGSTEADPYDGKISNESPVGKALLGRAAKEVVEVQVPDGVAKFEILEIRR"
     gene            5263..6792
                     /gene="lysS"
                     /locus_tag="KRL67_003218"
     CDS             5263..6792
                     /gene="lysS"
                     /locus_tag="KRL67_003218"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HBH1524064.1"
                     /translation="MKNNQQSNEEAQIQEDLSEVLQVRRDKLKKLQESGRDPFKESRY
                     DRTHYSMDIKDNFDSLEGKTTKIAGRIMSKRIQGKAGFIDIQDQEGRIQSYVRLDAIG
                     EEEYSVFSTYDIGDIVGIEGEIFKTKKGEISVKAKSVVLLCKSLQVLPEKYHGLKDQE
                     LRYRQRYVDLIVNPEVKNAFLIRTKALKALRAYLDDRGFLEVETPILNTIAGGANARP
                     FITNHNTLHIPMYLRIANELYLKRLIVGGFDKVYEMGRMFRNEGMDLKHNPEYTAIEL
                     YQAYADYTDMMEITENVIAHMAEVATGSMIVNYQGTEINFTPPWKRMSMEDCVKEYSG
                     VDFSTINTDEEALEVAREKGIEIKPGMRRGEVINAFFEEFGEDKLIQPTFITHHPVEV
                     SPLSKRNVEDPRRTDRFEAFANKWELANAFSELNDPIDQKGRFIDQLRKRELGDDEAF
                     EMDEDFLKALEVGLPPTGGLGIGIDRVIMLLTNSPSIRDVLLFPTMKPIDNNPNKDEE
                     N"
     gene            7101..7295
                     /locus_tag="KRL67_003219"
     CDS             7101..7295
                     /locus_tag="KRL67_003219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH1524065.1"
                     /translation="MFQVINFLCLFCVGFLVLDVMIYFCKDAMDIFEGTTNVNKVKSI
                     NVWADRVRANSYSYENKIAK"
BASE COUNT         2977 a          757 c         1495 g         2205 t
ORIGIN      
        1 attatttatt agaagaaaat aagtcatgaa atcatgtcat attagaggtg aaatatgcga
       61 gataaaataa tagagattat attaaataat aaatctgaat ttatttcagg agaagaatta
      121 tcaaaacaat taggagtgtc acgtgcagct atatggaaac atatgaaagc cttaaaagaa
      181 gagggatata atatagaatc tgtaaataag aaagggtatc gattagcgga aaatccaact
      241 gatttattaa gcccacagaa tatttactat aaattggata cagaatttat tggaaaaaat
      301 attgaacatc ttgaaactat agattcaaca aatgaacatg ctaaaaggac agctataaat
      361 agtgtagatg ggactgtgat tttaagtgaa catcaagata tggggaaagg acgattggga
      421 agaagatggg attcaaaacc tcatgaagga atctggatga gtattatatt aaaaccagat
      481 atggaaccat tcaaagctcc atttataact ttaatagcag gagcaagtgt aacgaaagct
      541 ttaaacaatc taggagcaaa tacattaata aaatggccaa atgatgttat tttaaatgga
      601 aaaaaaatat gtggtatatt aacagagtta tctgctgaaa ttgaaagaac aaactacatt
      661 gttcttggaa ttggaataaa tgttaaaata atggattttg aacaagaaat agctgacaaa
      721 gctacatcat tgtataaaga aggatatatg ttacccagag tagatattgt taggaatgta
      781 ttgactgaat ttgaaaaatt atatttagat tatactgtaa ataattccaa agaaaaaacc
      841 ttaaaaatat gtagagatta ttctgcaatt atagataaaa atatatatgt attaaaagga
      901 gataataaag aactagtgaa atgcttagac ataaatgaag aggggaattt attagttcaa
      961 agaagtgatg gaagtataaa agaaattatt tcaggtgaag tttctataag aggagaaaaa
     1021 ggatatgtat aataatattt tggatataga aggcttaaag gttgggcagg ttgaggatag
     1081 aaatggtctt actgggtgca cagtagtaat atgtgaagaa ggagctactt gcggtgttga
     1141 tgtaaggggg tctgctccag gaactagaga aaccgattta ttagaccctg taaatatggt
     1201 ccaaaaagtt catgcagtag ttttggctgg aggttctgca tatggtcttg aatccacttg
     1261 tggtgtaagt agatacttag aagaaaaagg aattggattt gatgtaggtg taggtaaagt
     1321 gcctatagtt gtaggagcag ttttatttga tttaggagta gggagcttta aatgtagacc
     1381 agatttaaag atgggttata gagcttgtga agttgcaaat actgatattt taaatcaagg
     1441 aaattatgga gcaggttgtg gagccactgt tggaaagata agaggtcagg aatatgctat
     1501 gaaaggcgga ataggtagct attctataaa attagataat ggtctagttg tatctgcatt
     1561 aattgctgta aatgctcttg gagatgttta tgaaaatggt aaagttatag caggtgcttt
     1621 aaatgataaa aagacagaaa caattgatac atataaatta atgaaacaag gtgaaagtaa
     1681 aggtggattt agtgtagata atactacaat tgggataatt gcaacaaatg ctaagttaac
     1741 caaagctgga tgtaaaaaga tatctcaaat gggacatgat ggatatgcta aagctatttt
     1801 tcctatccat acacctcatg atggagatac aatattcaca atatcaacag gagaaataga
     1861 agcagatata acattagtag gttcattggc tgtagaggtt gtggaaaaaa gtataataaa
     1921 tgcagtaaaa aaagcggata gtgtagaaaa tataatggca tataaagata taataaaaaa
     1981 acgagaaata tgatgaaatt agcatatttt tcttgtcaaa ttgttgacaa tggcaactca
     2041 tcttgataaa attattcttg ttagagcggg catcctacaa tggtagcttg actcataatt
     2101 ataagaaaaa aacgaaaaaa ttaaaaaatt aagtagtccg gcatcttaat gagctggaac
     2161 ggaggtgtat tttttatgat gaacacaagt gcatcagtaa gaaaagtaaa tgtaagaaag
     2221 atgactataa taggagtact atcagctatt tctattatgc tatctatgac tccattggga
     2281 tttattccag ttggacctac aaaagcaact ataatgcaca taccagttat tattggagct
     2341 attatggaag gaccaatagt tggggctgca ataggattta tatttgggat atcaagtctc
     2401 ttaaatgcta taacaaaccc aacagtaaca tcttttgttt ttataaatcc attagtttca
     2461 atattaccaa gagttatgat aggtgtatta gcttattatg tatatcagtt aataattaag
     2521 gctacaaata aggtatacat gtcaggttta attacagggg caataggttc tctgttaaat
     2581 acagctggag ttttaggaat gatatatgtc ttatatgctg acaagtatct tcatgcaatg
     2641 ggtaaaagtg gttcagcagg aaaagtaata atggctcttg ccgcagcaaa tggagtgcca
     2701 gaagcaatag taggggcttt agtagtagct gctgttgcca ctgtacttaa gaaaagcaaa
     2761 aaatagaaag aaggttaatg acatatgctt ctagtatttg atgttggaaa tactaatatg
     2821 gttttaggta tatataaagg tgacaaatta gttaattact ggagaattaa aacagataga
     2881 gaaaaaacgt ctgatgaata tggaatcctt ataagtaacc tatttgatta tgataatgtg
     2941 aatataagtg atattgatga tgttataata tcatctgtag ttccaaatgt tatgcattct
     3001 cttgaaaact tttgtataaa atactgtaaa aaacagccat taatagtagg tccaggcata
     3061 aaaacaggtc taaatataaa atatgataat ccaaaacaag ttggggcaga cagaatagtt
     3121 aatgctgtag cagggataga aaagtatgga gcaccaagta tacttgttga ttttggaaca
     3181 gcaactacat tttgtgctat ctctgaaaaa ggtgaatatt tgggtggaac aatagcacca
     3241 ggaataaaaa tatctagtga ggcgttattt caaagtgcat ctaaattacc tagagtagaa
     3301 ttagctaaac caggtatgac tatttgtaag agtactgtat cagctatgca atctggaata
     3361 atttatggat atgttggttt agttgacaaa ataataagta tgatgaagaa agaattgaat
     3421 tgtgatgatg tcaaggttat agctacaggt ggattagcta aactgattgc ttcagagacg
     3481 aaaagtatag attatgtaga tggtttttta acactagaag gattgagaat aatatatgaa
     3541 aaaaaccaag aataattaat atacagggaa ttgggttata tatccaattc cctttttatg
     3601 atagatttct agatatatgt ggtatattat aatgaggaaa taagaattta aagttaaatc
     3661 acaaaataaa ggcaaaaata ggagaaaaac aatgaaaata ggaaatttaa aattgaaaaa
     3721 taaagttttt ctatcaccta tggcaggtgt aacagacctt ccatttagat taatttgtaa
     3781 agaacaggga tgtgggcttt tatatacaga aatgataaat ggtaaggcac tttgctatga
     3841 tgatgaaaat actaaaaaaa tgcttaagat agaagaagaa gaacatcctg tagcagttca
     3901 gatattcggc tcagagccag agtttatggg aagagcagca gagataatga atgattattc
     3961 aaatgaaatt ctcgatataa atatggggtg tccagctcca aaagtagtta aaaatggaga
     4021 tggttcagca cttatgaaaa atcctaagct tgcagaagag gtattgaggg cagtagtgaa
     4081 gaactcaaaa aagccagtga ctttaaagat tcgaaaaggt tgggatgata atagtgtaaa
     4141 tgctgttgaa atagcaaaga tagcagagga ttgtggaata agcgctttag caatacatgg
     4201 aagaactaga gaacagtttt attcaggtaa agcagactgg gatataattg cagagattaa
     4261 gaaaaatttg agtatacctg ttataggaaa tggggatgta tttacaatag aagattcaat
     4321 aaatatgtta gacaaaactg gttgtgatgc tataatgata ggtaggggtg ctcaaggtaa
     4381 cccttggata tttaaaagaa taaaccatta tatgaataca ggcgagattt tgccagagcc
     4441 aactcttaac gaaaaaataa gtactgccat taagcattta aaacttgcag tagaagaaca
     4501 tggagagtat gtagctgtaa gggaaatgag aaaacacatc gcttggtatt taaaaggact
     4561 tagaaactct gcaaaattaa gggatgaaat taacaaaata gaagactatc aagaagttgt
     4621 gtcgaaattg gagtactata tggaagactc cttgacataa aaataatagt gacttataat
     4681 atagcgcata aattaaaaaa agattataaa atttttaaaa tataattagg aaaattccta
     4741 agaaatttgt gaaggagttg tatttataat ggaagaaaat aaagaatttt tattgacaca
     4801 agagggatat gataaattag aagaagaatt agaaaactta aaggttgtaa agagaaaaga
     4861 agttgcagag agaataaaag ttgctatatc ttttggtgat ttatctgaaa atgctgagta
     4921 tgatgaagct aaaaaggaac aagcacaagt tgaagaaaga attttaaaat tagaaaatat
     4981 ggttagaaaa gctgttatca tagatgaaag caaaatagac cttaatgttg ttacaatagg
     5041 atcaatagtt aaagttaagg atttagaatt tgatgaagag gttgaatata ctatagttgg
     5101 ttcgacagaa gcagacccat atgatggtaa aatatcaaac gaatctcctg taggaaaagc
     5161 acttttaggt agagctgcga aagaagttgt ggaagtgcaa gttcctgatg gagttgctaa
     5221 gtttgaaata ttagaaataa gaagataatt tggaggaaaa aaatgaaaaa taatcaacaa
     5281 agcaatgaag aagcacaaat tcaagaagac cttagtgaag tacttcaagt aagaagagat
     5341 aaactaaaaa aattacaaga gtcaggtaga gatcctttta aagaaagtag atatgataga
     5401 actcattatt ctatggatat aaaagataac tttgattctc ttgaaggtaa aactacaaaa
     5461 atagcaggac gtataatgag caagagaata caagggaaag ctggttttat agatattcaa
     5521 gaccaagagg gaagaatcca atcttatgta agactagatg ctataggaga ggaagaatat
     5581 agtgtatttt caacatatga tattggagat atagttggaa ttgaagggga aatctttaaa
     5641 actaaaaaag gtgaaatatc tgtaaaggct aaatctgtag tattactatg taaatcttta
     5701 caagttttac cagaaaaata tcatggatta aaagaccaag aactaagata tagacaaaga
     5761 tatgttgact taatagtaaa tccagaagta aaaaatgctt ttttaattag aacaaaagca
     5821 ttaaaagcat taagagcata cttagatgat agaggatttt tagaagttga gactccaata
     5881 ttaaatacta tagcaggtgg agcaaatgct agaccattta taactaatca taatacttta
     5941 catataccaa tgtatttaag aatagcgaat gaattgtatt taaaaagact tattgttggt
     6001 ggatttgata aagtttatga aatgggaaga atgtttagaa atgaaggtat ggatttaaaa
     6061 cataatcctg aatatactgc aatagaatta tatcaagcat atgcagacta tactgatatg
     6121 atggaaataa cagaaaatgt tattgcacat atggctgaag ttgctacagg aagtatgata
     6181 gttaactatc aaggaacaga aataaacttt acaccacctt ggaaaagaat gagtatggaa
     6241 gattgcgtta aagaatattc tggtgtggat ttttccacta taaatacaga tgaagaggca
     6301 ctagaagttg ctagagaaaa aggtatagag ataaaacctg gaatgagaag aggagaagta
     6361 ataaatgctt tctttgaaga atttggagaa gataaattaa tccaaccaac atttataact
     6421 catcatccag ttgaagtatc tccattatct aagagaaatg ttgaagaccc aagaagaact
     6481 gatagatttg aggcatttgc taataagtgg gagttagcta atgcattttc tgaacttaat
     6541 gaccctattg accaaaaagg aagatttata gaccaattga gaaaaagaga attaggtgat
     6601 gatgaagctt ttgaaatgga tgaagatttc ttaaaagcat tagaggtagg tctacctcct
     6661 acaggaggac taggaatagg tatagataga gtaattatgt tattaactaa ctctccatct
     6721 ataagagatg tattattatt ccctactatg aaacctattg ataacaatcc aaataaagat
     6781 gaagaaaact aagaaatatt agcattctac taagttttaa ataaagttta gctattaaat
     6841 tagcaataaa tagctaaatt ttatttaaaa tgtcagtgta gaatgctttt tatatgcttc
     6901 aaaaattctt ttggatattt aatttataaa ttaccaacaa acgttagaaa ttgatattag
     6961 tttttgttga ttgagacttt atatttagaa aatatgattg aaagtaattt attatgaaaa
     7021 tatttagaat aatttatgag aaggtaaaaa gaataattta tgagaatact atatataaaa
     7081 aattattggg ggaatgtctg atgtttcaag taattaattt tttatgttta ttttgtgttg
     7141 gatttttggt tcttgatgtt atgatatatt tttgtaagga tgcaatggat atttttgagg
     7201 gtacaactaa tgttaataaa gttaagagta taaatgtttg ggcagataga gtcagagcta
     7261 attcatattc ttatgaaaat aaaattgcaa aataggtaaa attatatatg catgtattgt
     7321 ttgtaatatc aagggttata tctattagtt ttaattaaag ttattaaaaa aaaacaaaag
     7381 ttataacctt ttttaaaata ttttaaaata aaaaacaaaa aaagtattga cgaa
//
LOCUS       DAEPXF010000179         7423 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain HB27
            SAMN13376389-rid12479423.denovo.186, whole genome shotgun sequence.
ACCESSION   DAEPXF010000179 DAEPXF010000000
VERSION     DAEPXF010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13376389
            Sequence Read Archive: SRR10518432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7423)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7423)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001069624.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001069624.1
            Genome Coverage       :: 315x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/15/2021 01:54:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,165
            CDSs (total)                      :: 4,076
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 89
            rRNAs                             :: 10, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 10 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 19 of 69
            Pseudo Genes (incomplete)         :: 44 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 10 of 69
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7423
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13376389-rid12479423.denovo.186"
                     /strain="HB27"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="China: Hebei"
                     /collection_date="2017"
     gene            64..405
                     /locus_tag="KRL67_003220"
     CDS             64..405
                     /locus_tag="KRL67_003220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family transcriptional regulator"
                     /protein_id="HBH1524066.1"
                     /translation="MKEENFINVEYEDKRFSINSKEVLYIEKQEYEVKIYTESGNYDV
                     GENAPCIRKLLKEENFFKCSNSYIVNLDKCEFYLSNNCIFVNNNFIRILGSERKKKLK
                     KKLEKLYDYED"
     gene            556..969
                     /locus_tag="KRL67_003221"
     CDS             556..969
                     /locus_tag="KRL67_003221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1653 domain-containing protein"
                     /protein_id="HBH1524067.1"
                     /translation="MLELKNNSGTSERKLKYPCIYKHFKGKYYATMGLSKAIDNIENI
                     CEIYGRENLIQNRNKYKLVVRHTEREEDIYVYRDLHGNFYHKKEEDTNDLVLYKTLYD
                     DTGIFARPLDMFLEKVDTDKYVNSIQEYRFEEVYK"
     gene            1171..1812
                     /locus_tag="KRL67_003222"
     CDS             1171..1812
                     /locus_tag="KRL67_003222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HBH1524068.1"
                     /translation="MGNTFYIVRHGQTDWNILGKTQGHGNSDLTPQGIEQAKELSEDI
                     GKYSIDYIFSSDLGRAMQTAQILGDKLNIEVQKTEALREMGFGVWEGLLIKEIQKDYS
                     DIYATWRNEPHLVNIPEGETLKIIKERVDAFIKELNEKYDNKNIILVTHSITLRVMLL
                     SFLESGMENIYRIKQDNTALNIVEFKDYGPVIIKMNDTSHIKNHVKINNSALE"
     gene            1836..3089
                     /locus_tag="KRL67_003223"
     CDS             1836..3089
                     /locus_tag="KRL67_003223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HBH1524069.1"
                     /translation="MSKVIVVGGGASGMMAALSASKNNNEVILVERNGELGRKLRATG
                     GGRCNFTNNREIEDFFDKVVSNKKFLYSSFYTFTNKDLISYFESRNLEYKIEEENDHK
                     VYTKNDKSIEVIEVLNKDLLNHNVKIMYNKKVIDIITEEIALKDDSNKDKSKYLIKGI
                     ILDNGDKILGDKVIISTGGVSYSKTGSDGSMYKILKKHGHTLNKLYPALVPLTIEEKW
                     IKDLQGISMKNVEISCKIKKRKISKFGDMLFAHFGITGPCVLIMSSYINKIIEKEKIE
                     LNIDFLPNLSTDEISSIIRAFPNKNVLNNLKQILPQNFLKEIFSLLSLGDKKASDLSK
                     ADEIRIIEYIKNMKLTCNGTTGINTGMVTSGGISVKEINSSTMESKLVKNLFFTGEVI
                     DIDAETGGYNLQIAFSTGYLAGISV"
     gene            3135..3785
                     /locus_tag="KRL67_003224"
     CDS             3135..3785
                     /locus_tag="KRL67_003224"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HBH1524070.1"
                     /translation="MGNLVIAVDGPAGAGKSTIAKIVAKKLNINYIDTGAMYRAVTYK
                     CLKSGIDVNNEKEVIQTAENSDIDFKDNNIYLDKEVINEEIRTIEVSNNVSNVAKIKE
                     VRQLMVEVQRKIGMKSSVILDGRDIGSYVFPNADYKFFLVATPEERGNRRYKELCNKG
                     YNTTLEEVIEDIIRRDEIDSNREFAPLVKANDALEIDTTGKTIEEVVEEVVSKINL"
     gene            3836..4432
                     /locus_tag="KRL67_003225"
     CDS             3836..4432
                     /locus_tag="KRL67_003225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HBH1524071.1"
                     /translation="MNFYRFVINIFKGFSKIFFKYEVIGAENIPDRGNIVIASNHKSN
                     LDPIFLAAAIENREIAAIAKKELFKVKPLGFILKKLHVMPINREKPDVSTIKTILRSV
                     RDGYVLGIFPEGTRIKGDSFGKAKAGLSVFTIKSKSKVVPVSIISKYKLFSKVIVYIG
                     EPISFEEHFKEKLSNGDHERISQEILEVIKQNYFKYSK"
     gene            4445..5284
                     /locus_tag="KRL67_003226"
     CDS             4445..5284
                     /locus_tag="KRL67_003226"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HBH1524072.1"
                     /translation="MNIKIAKNAGFCFGVKRAMKMAWDEVEKNDSGIYALGPLIHNKQ
                     AVAKYEEKGLKTVNEIDTIPNHENMIIRSHGVPENIYKEAKDKKLKIVDTTCPFVKKI
                     HTVVSEYHNKGYEIIVIGDMKHPEVIGINGWCENSAIIIKTLEQMENMEFDNSKKYCL
                     VAQTTINPELYISIVNKLSDKLEEIVFNDTICSATKTRQESAKELAKEVDCMIVIGGK
                     HSSNTQKLVKVCEDLVPTFAIETKDELDVNTLKKYKNLGITAGASTPNWIIEEVVTFL
                     ENL"
     gene            5531..6685
                     /locus_tag="KRL67_003227"
     CDS             5531..6685
                     /locus_tag="KRL67_003227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenic efflux protein"
                     /protein_id="HBH1524073.1"
                     /translation="MEMLISASEEAFLHVGSMIGFFILLFGYINYKTSGNFTNIISKN
                     RKFQPLIGALIGAIPGCGGSLAIMPLYINGKLSFGAIIASLIASMGDAAFVLISSNIK
                     MYFFVTIVSTITGIITGQLVDYFKLEEKLGLKNRRKSDKYDISKNNSKENKEQHNHDE
                     IILDTLAKSHGNTNRLAFIITHGKGYKIYIGIIVIGFIFMLLAHSGLNLPIIEKLHSL
                     EEAIAVIGILFSIIYMWCFKKVFKNSNQKEEENKKISLREMLIHSVGEISFVITWIFI
                     AYLIYDLIILILGGDEYLVKLVLSTGVISVFIGAGLGLIPGCGIQIVLMSFYLKGTIP
                     LGAVIANSISQDGDALFPLLAMDKKSSLWAMIITTIPAILVGMIVYAFLG"
     gene            6991..7167
                     /locus_tag="KRL67_003228"
                     /pseudo
     CDS             6991..7167
                     /locus_tag="KRL67_003228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728625.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
BASE COUNT         3079 a          708 c         1303 g         2333 t
ORIGIN      
        1 cgatagatta tattgataaa aaacactaaa attaaattaa taaaagtact tggagggatg
       61 gaaatgaaag aggaaaattt tattaatgtt gaatacgagg ataaacgttt ttcaattaat
      121 agtaaagaag ttttgtacat agaaaagcaa gaatatgaag taaagattta tactgaaagt
      181 ggtaattatg acgttggaga aaatgctcct tgtataagaa aactattgaa agaagaaaat
      241 tttttcaaat gcagtaatag ttatattgta aatctagaca agtgtgaatt ttatcttagc
      301 aataattgta tttttgtgaa taataatttt ataagaattt taggttcaga aagaaaaaag
      361 aaattaaaaa agaaattaga aaaattatat gattatgaag attaatattg agaggctgta
      421 ttttatacag cctctttatt tattcattgt attattaata aattttaaat tatgataaaa
      481 tataaaacaa gtaacttatt tcagaataaa atacttttta aaagaaaggg acgagatatg
      541 ggagattata atcaaatgct tgaattaaag aataactcag gtactagtga aagaaagtta
      601 aaatatcctt gtatctataa acatttcaaa gggaaatatt atgctacaat gggtcttagc
      661 aaggctattg ataatataga aaacatatgt gaaatatatg gtagagaaaa tttaatccaa
      721 aacagaaata aatataaatt agttgttagg catactgaaa gagaagaaga tatctatgtt
      781 tatagggatt tacatggaaa cttttatcat aaaaaagaag aagatactaa tgatttagtt
      841 ttatacaaga ctttatatga tgatacagga atatttgcaa gacctttgga tatgttttta
      901 gaaaaagtag atacagataa gtatgtcaat tcaattcaag aatacagatt tgaagaagta
      961 tataaataat ataagagaaa tatgtttata aatttagaaa aattctttaa cagtacaata
     1021 atttgcagaa atacaaatga gatttattgt aaataatatg ttagattaga ttataaaata
     1081 gaaacaaagt aaagtattat atgatataat taagttttga gaagtgataa tttatattta
     1141 ataaaaaata ggcatatatt aggaggacat atgggaaata ctttttatat agtaagacat
     1201 ggacaaacgg attggaacat attgggaaaa acacaaggtc atggtaattc tgaccttacg
     1261 cctcaaggta tagaacaggc aaaagaactt tctgaagata ttggcaagta ttcaattgat
     1321 tatattttta gtagcgacct tgggagagca atgcaaacag ctcaaatttt aggagataaa
     1381 ctcaatattg aagttcaaaa aactgaggca cttagagaaa tgggattcgg agtttgggaa
     1441 ggccttttaa taaaagaaat tcaaaaagat tattcagata tatatgcaac atggagaaat
     1501 gagccacact tagtaaatat accagaaggg gaaactctta agattataaa ggaaagagta
     1561 gatgctttta taaaggaact aaatgaaaaa tatgacaata aaaatataat tcttgttaca
     1621 cattcaatta cattgagggt tatgttgttg tcatttttag aatcaggtat ggaaaatata
     1681 tatagaatta agcaagataa tactgcttta aatatagtag aatttaaaga ttatggacct
     1741 gttataataa aaatgaatga tacaagtcat ataaaaaatc atgtaaagat aaataactct
     1801 gcactggagt aggaatcaat tataaggaga aatttatgag taaagttata gtagttggtg
     1861 gaggagcttc tggaatgatg gcagctttgt cagcttcaaa aaacaataat gaagtaatat
     1921 tagttgagag aaatggagaa ttaggaagaa aacttagggc aactggtggc ggtagatgca
     1981 attttaccaa taatagagag atagaagatt tttttgataa agttgttagt aataaaaaat
     2041 ttctctatag tagtttctat acatttacaa ataaagacct tatttcgtat tttgaaagta
     2101 gaaatttgga atataagatt gaagaagaaa atgaccacaa agtatatact aaaaatgata
     2161 aatctataga agtaatagaa gtattaaata aagatttact caatcacaat gtaaagataa
     2221 tgtataataa aaaagttata gatattataa ctgaagaaat tgctctaaaa gatgattcta
     2281 ataaggacaa gtcaaagtat ctaataaaag ggattatttt agataatgga gacaagatac
     2341 ttggagacaa ggttattata tcaactgggg gagtgagtta ttcaaaaacg ggttcagatg
     2401 gttcaatgta caagattctt aaaaagcatg gacatacatt aaacaaacta tatcctgctt
     2461 tagttccact tacaatagag gaaaaatgga ttaaagattt acaaggaata tccatgaaga
     2521 atgtggaaat atcttgtaag attaagaaaa ggaaaatatc aaaatttgga gacatgttat
     2581 ttgcacattt tggtataaca ggaccttgtg ttcttataat gtcttcttat attaataaga
     2641 ttattgaaaa ggaaaagata gaattaaata tagatttctt accaaattta agtactgatg
     2701 aaatctctag tataatcaga gcatttccaa ataaaaatgt tctaaataat cttaagcaaa
     2761 tactacctca aaatttctta aaagagattt tttctctatt atctttaggt gataaaaagg
     2821 caagtgattt gtctaaggca gatgaaatta gaattattga gtatataaaa aatatgaagc
     2881 taacttgtaa tggaactaca ggtataaaca ctggtatggt tacttctggt ggtatttcag
     2941 ttaaagaaat taattcttct actatggaat ctaaattagt taagaactta ttttttacag
     3001 gtgaagtaat tgatatagat gcagaaactg gtggatataa tttacaaata gcattttcaa
     3061 ctggatatct tgcgggtatt agtgtataaa tataattttt taataattaa atatgaataa
     3121 atagaggtgt atatatgggt aatttagtta tagctgtaga tggtccagca ggagctggaa
     3181 aaagtacaat agccaagata gtagcaaaaa agctaaatat aaattatata gatacaggtg
     3241 ctatgtacag agcagttact tataagtgtt taaaaagtgg tattgatgtc aataatgaaa
     3301 aagaagttat tcaaacagct gaaaattcgg atatagattt taaagataac aatatatatt
     3361 tggacaaaga agttataaat gaagaaataa gaacaataga agtaagtaat aatgtttcta
     3421 atgtagcaaa aataaaagaa gtaagacagt tgatggttga agttcaacga aaaataggaa
     3481 tgaaaagttc agtaatacta gatggtagag atataggttc atatgttttc ccaaatgcag
     3541 attacaaatt ctttttagta gctactccag aagaaagagg aaatagacgc tataaagaac
     3601 tttgtaataa aggatataat actactttag aagaagtaat tgaggatata ataagacgag
     3661 atgaaattga ttcaaataga gaatttgcac cattagtaaa agctaatgat gcactagaaa
     3721 tagatactac aggaaaaact atagaagaag ttgtagaaga agttgtatct aaaataaatc
     3781 tttagttttt tatgcatcat aatgaaaata aatgtagtgt tgaagggaga taaacgtgaa
     3841 tttttataga tttgtaataa atatttttaa gggtttttct aaaatatttt ttaaatatga
     3901 agttataggt gcagaaaata ttccagatag aggaaatata gtaatagcat caaatcataa
     3961 atcgaatctt gaccctatat tcttggctgc tgccatagaa aatagagaaa tagctgcaat
     4021 tgcaaagaaa gaattgttta aagtgaaacc gttgggattt atattaaaaa aattgcatgt
     4081 tatgcctata aatagagaaa agccagatgt atctacaata aaaactattt taaggtcagt
     4141 tagagatggt tatgtgttag gaatatttcc agagggtact agaataaaag gagattcttt
     4201 tggtaaggct aaggcaggtc tttctgtatt tacaataaaa agtaaatcta aagttgttcc
     4261 agtatcaata atatccaaat ataaactttt tagtaaagta attgtatata ttggagaacc
     4321 aatatcattt gaagaacatt ttaaggaaaa attgagtaat ggtgaccatg aaaggatatc
     4381 acaagagatt ttagaagtga taaaacaaaa ttactttaaa tattcaaaat aagggggtaa
     4441 agttatgaac ataaaaatag caaaaaatgc tggcttttgt tttggagtaa aaagagcaat
     4501 gaaaatggca tgggatgaag ttgaaaaaaa tgattctggc atatatgctt taggtcctct
     4561 tattcataac aagcaagcag tagcaaaata tgaagaaaaa ggtcttaaga ctgtgaatga
     4621 aattgataca ataccaaatc atgaaaatat gataataagg tctcatggag ttcctgaaaa
     4681 tatatataag gaagccaaag ataaaaaatt aaaaattgtg gatactacat gtccatttgt
     4741 aaagaaaata catacagtag taagtgagta tcataataaa ggctatgaaa taatagttat
     4801 tggagatatg aagcatccag aagttatagg tataaatgga tggtgtgaaa attcagctat
     4861 aataataaag actttagaac aaatggaaaa tatggaattt gataactcta aaaaatattg
     4921 tcttgttgca cagactacaa taaatccaga gttgtatata agtatagtta ataaactatc
     4981 agataaatta gaagaaatag tatttaatga tacaatttgt tcagctacaa aaacaagaca
     5041 agaatcagct aaagagttag ctaaagaagt tgattgtatg atagttatag gaggtaaaca
     5101 cagttcaaat actcaaaagt tagtaaaggt ttgtgaagat ttagtaccaa cttttgcaat
     5161 agagactaaa gatgaattgg atgtaaatac tcttaaaaaa tataaaaatt tgggtattac
     5221 agcaggggca tctacaccta attggataat agaagaagtt gtaacattcc ttgaaaattt
     5281 ataatttgat aggtaataaa aataggtgga cattgttata atatagaact tatcataata
     5341 tccacctttg atttcatatt acatgtggta aatagataac ctaatatcaa aaagtgtgta
     5401 tcttaagatg aaaaactaat agtataagat gcgcactttt taatattaga aattcaaaag
     5461 aaatattgat attgattatc aatagaaaat ataatataat tattgcaaaa ttagtctagg
     5521 aggtaaatta atggaaatgt tgatatccgc ttctgaagag gcgtttttac atgtaggttc
     5581 tatgattgga ttttttattt tgttatttgg atatattaat tataaaacta gtggaaattt
     5641 tactaatatc atatcaaaaa acagaaaatt tcaaccttta ataggtgctt taattggagc
     5701 tataccaggt tgtggcggaa gtttggcaat aatgccattg tatataaatg gaaaattaag
     5761 ctttggagca ataattgcaa gtttaatagc tagtatggga gatgcagcat ttgtacttat
     5821 ttcatcaaat attaaaatgt atttttttgt cactatagtt agtactatta caggaataat
     5881 aactggacaa ctagtggatt actttaaact agaagaaaaa ttagggttaa aaaaccgtag
     5941 aaaatcagat aaatacgata taagtaaaaa taactctaaa gaaaataaag aacaacataa
     6001 tcatgatgaa ataattttag acactctagc taaatctcat ggaaatacaa atagacttgc
     6061 attcataata acacatggaa agggatataa aatttatata ggaattatag taatcggttt
     6121 tatatttatg ttacttgctc actctggact taatcttcct ataattgaga agttacattc
     6181 attggaggaa gcaatagcag tgattggaat attattttca ataatttata tgtggtgttt
     6241 taaaaaggta tttaaaaatt ctaatcaaaa agaggaagaa aataaaaaga tttctttacg
     6301 agaaatgctt atacattctg ttggagaaat atcatttgta attacatgga tttttattgc
     6361 atatcttatt tacgatttga taatattaat tttaggtgga gatgaatact tagttaaact
     6421 agtattatca acaggtgtaa taagtgtatt tataggtgca ggtcttgggc ttattccagg
     6481 ttgtggtata caaattgtgc ttatgtcatt ttatttgaag ggaactatac cacttggagc
     6541 agtgatagcc aactctattt ctcaagatgg agatgcactt ttcccacttt tagcaatgga
     6601 taaaaaatct tctttatggg ctatgattat tactactata ccagcaatac ttgtaggtat
     6661 gatagtttat gcatttttag gctaaatata attataaata aagacgtatt aaaaaaataa
     6721 taaagcacat aaatttattt ataagaatag tgaagattaa gtatagaaat aaaaatttca
     6781 atatgaatta aattattgct taaatattaa tgagaacatg ggagataata ttattcctca
     6841 tgttctctct atttaattaa aggaattata gaacaaattt caagtgatac tttctatcaa
     6901 aactcatata taatatatat taataaatta aactataata tgtaatacag ccagtatatt
     6961 gatataaaaa acttaggagg agttgtaaac atgggtaatt taaaaagaca gtgtgatgtt
     7021 tcaagtggaa gagaaaaagc tgacattgta ctaaaaaatg ggacaatcat aaatgtattt
     7081 acagaagagt taataacagg agatgtggca atagtaggag acactattgt tggaattggc
     7141 gattacaagg gcaatgtaga aattgattaa aaaaatagct acgcacactc gtgactttag
     7201 tcatgagtta gtagctaaaa tagtcagcat atagggaaac ttatatgtag agataggata
     7261 gaacctatcc aatactactt gaattgctgg aaacccctaa agctaaccaa actaaaacat
     7321 aaggatgaaa taaacctaag tgtgaaagtt acgaaagtag aaaaaattgg ttagatggta
     7381 taaggttaaa tcctaagtac tttaaaatgg gcaatcagca ggt
//
LOCUS       DAEPXF010000180         7308 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain HB27
            SAMN13376389-rid12479423.denovo.187, whole genome shotgun sequence.
ACCESSION   DAEPXF010000180 DAEPXF010000000
VERSION     DAEPXF010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13376389
            Sequence Read Archive: SRR10518432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7308)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7308)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001069624.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001069624.1
            Genome Coverage       :: 315x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/15/2021 01:54:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,165
            CDSs (total)                      :: 4,076
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 89
            rRNAs                             :: 10, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 10 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 19 of 69
            Pseudo Genes (incomplete)         :: 44 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 10 of 69
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7308
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13376389-rid12479423.denovo.187"
                     /strain="HB27"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="China: Hebei"
                     /collection_date="2017"
     gene            143..1414
                     /gene="sleC"
                     /locus_tag="KRL67_003229"
     CDS             143..1414
                     /gene="sleC"
                     /locus_tag="KRL67_003229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore cortex-lytic protein SleC"
                     /protein_id="HBH1524074.1"
                     /translation="MQDGFLTVSIIDATNNRPIQNAVVNIYSMSNGSQSSSTLYQNLR
                     SNESGQVTGLVLPAPDVDYSLQPSDVRPYSQYIVEAIADGYETVVIEGTQLLATIEAR
                     QGVPMSPRTRSKRSFSRQSELIFDIGEHTLYGTYPPKIPESNLKPLPPPTGFVVLDNP
                     VVPEFIVVHDGLPEDSSAPNYWIPFKEYIKNIASSEIYSTWPEQTIYANVIAIISFTL
                     NRVFTEWYRNKGYNFTITSTTAYDHKFINNRNLFEPINVVVDAIFNTFIKRPPTSRQP
                     LLAQYCDGQKSQCPDQMTQWGSKDLGDQGYDYESILRYFYGDEIVFERAPIVSGVPVS
                     FPGTTLQVGSSGQYVRTIQNQLNAISNSYPAVPKVIEDGIYGTDTENAVKIFQGIFGL
                     PQSGVVDFKTWYEISRVYVATTRIASLNPLI"
     gene            complement(1885..2541)
                     /locus_tag="KRL67_003230"
     CDS             complement(1885..2541)
                     /locus_tag="KRL67_003230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH1524075.1"
                     /translation="MKKGDFIWSGVLICIIAVLAIPSSREVFMEATGAHPYIGGFLKF
                     AVLATMGDLLGARILYKDWKIPVGVFYRAIVWGIIGVVTTYGMSIYSSGVGQAQVDGM
                     LPFEGIAFANALFKSATMNILMSPTVFLFHKVMDCFIDKKYEVGKGIKVKLKDVVNIV
                     DWNAYLGFSVLKTIPFFWIPCHTIVFLFPAEYRVIASAFASIALGLILALANKSKVAV
                     "
     gene            complement(3754..4164)
                     /locus_tag="KRL67_003231"
     CDS             complement(3754..4164)
                     /locus_tag="KRL67_003231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH1524076.1"
                     /translation="MKIKKSLISAMMVSAMVLGGTGAVFANGNDSSNIALPKLESLIK
                     SGITDPTELEKYGVEHYTYEEYAKHIQELKDYAKDPNAVKDVSQKDLEETIKKMEQEL
                     EKIKTEGLKIMKPITIKNEDGSQISIAYNYAAID"
     gene            complement(4380..5762)
                     /locus_tag="KRL67_003232"
     CDS             complement(4380..5762)
                     /locus_tag="KRL67_003232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M56 family metallopeptidase"
                     /protein_id="HBH1524077.1"
                     /translation="MFIKLLEMSFTSGVLLLIVIFIRACLFKKLPKKVFVLLWLIVLF
                     KLLIPVSLNSGFSIYSIIESTFDVELGNTSTENYTLKKASDSAGKNNSDEFEIMSSIE
                     NIMMLEMLGILSFIVTIMFSILLCFRYFKIINLFKTSIHIKNHEFIKKWMCKNSRFRR
                     FIVCISEFTRTPLTSGILKPRIILPKQMNYANNQTLDFVLTHEMVHINSFDNLLKLFM
                     LVALVVHWFNPLVWVMFFLLNKDIEFSCDEKVMSILGEEYRADYATTLISLAEENNPF
                     SMTICSGFGKTKIEKRIVNIMKFKKSTYVTFLVSTVLVCGTGLVFATENKEINEVESF
                     GLTKNEQSDKIGFSISSKSTDLYSPKLESDILVSKAKSDGETHFSTAKVSVIEFREHE
                     ESSTSKVTNSENSEETYVNKSSNSKDVLYTDSGEVSIERSNYNDNRFVISRKGNLLAV
                     NKEQYRTWIE"
     gene            complement(5773..6132)
                     /locus_tag="KRL67_003233"
     CDS             complement(5773..6132)
                     /locus_tag="KRL67_003233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HBH1524078.1"
                     /translation="MEIKLFDSELKVMEVLWERGESKAGELVKILKEETGWNRNTTYT
                     VIKKLVSKGAIERIEPNFVCKALISKEEVQKQETETLINKMFGGSSEKFLSAFINKEN
                     LSNDEITKLKQIVEKLK"
     gene            complement(6337..7116)
                     /locus_tag="KRL67_003234"
     CDS             complement(6337..7116)
                     /locus_tag="KRL67_003234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HBH1524079.1"
                     /translation="MVSRQELKRVSKAQLSGNWGICAISMAVYIALLVLISIVGLPFE
                     PLTTIVVAVIAFCVSAGFTCMFLKITKDEKVKIGDIFVSGRIYLRSFGFYLFESIIVY
                     IYSFIITIIGAFLSFGVLLTSGAIEYLSSDNVGIGTIISTGITLVLIILVLMLPVYIL
                     LLYVSQATFLICEDKDNMGVFKSMGASLDMMKGNVLKLFILNFSFIGWYILSIATLGI
                     GLLWLIPYVGVTNCNFYRQLVKENPDVEDILLDKEKSYTMY"
BASE COUNT         2575 a         1220 c          758 g         2755 t
ORIGIN      
        1 ttattttaaa ttatttaaat acaaatatac tcaaatactc aaaaaaattt aaatttatat
       61 ctatattatt taacaaattt caagctatat tcatatcttg aaatataagg gaactaaatt
      121 catttaaaga aagggtgatt tgatgcaaga tggtttctta acagtaagca taattgatgc
      181 tactaacaat agacctattc aaaatgcagt agtaaacata tattcaatgt ctaatggtag
      241 ccaaagctca tcaactttat accaaaacct aagaagtaat gaatctggtc aagtgactgg
      301 tttagtatta ccagcaccag atgtagatta ctctctacag ccatctgatg ttagacctta
      361 tagccagtat atagtggaag ccatagcaga tggatatgag acagttgtca tagaaggaac
      421 acagttgtta gctacaattg aagcaagaca aggagttccc atgtctccta gaacaagaag
      481 taagcgttct ttctcaagac aaagtgagtt gatttttgac ataggagaac atactctata
      541 tggtacttat cctcctaaga ttccagaaag taacttaaaa ccattgccac ctcctactgg
      601 tttcgttgta ttagataacc ctgtagtgcc tgaatttata gtagttcacg atggtttgcc
      661 agaagattca tcagctccaa actattggat tccatttaaa gagtacatta aaaacatagc
      721 atcatctgaa atatattcaa cttggcctga acagacaatc tatgcaaatg taatagctat
      781 aatatcattt actttaaaca gagtctttac tgaatggtat agaaataaag gatataactt
      841 tacgataact tcaacaacag catacgacca taagtttata aataatagaa acttatttga
      901 accaattaac gtagttgtag atgctatttt taatactttt ataaaaagac ctcctacatc
      961 aagacagcca ctattagcac aatattgtga tggacaaaaa tcccaatgtc ctgaccaaat
     1021 gactcaatgg ggcagtaaag acttaggtga ccaaggatat gactatgaga gtatactaag
     1081 atatttctat ggagatgaga tagtatttga aagagcgcct atagtaagtg gtgtaccagt
     1141 gtcattcccc ggtactacac tacaagttgg ttcttctggg cagtatgtta gaaccataca
     1201 aaatcagtta aatgctatat ctaattctta tccagctgta ccaaaagtta tagaagatgg
     1261 tatctatgga acagatacag aaaacgctgt taagatattc caaggaatct ttggtttacc
     1321 tcaaagcgga gtagtagatt ttaaaacatg gtatgaaata tccagagttt atgtagctac
     1381 aactagaata gcttctttaa atcctttaat ttaaagcttg atttagaaca taaactcaaa
     1441 ttattgaata aaattattaa tttaaaataa aaatggctgt tatgaactaa tatataccat
     1501 aagtattttt acttataagg attgtagtct taacagccat tttttagtta taacttgttt
     1561 tttttagaat cttaaaactt taaatggggg attttaagct tcttaaaaac agtgcttagc
     1621 aacttagatt tctttaaaat attatattta tttttaagtt tgataaaatg ctattctaac
     1681 gaaaagctta ctttttattt aaaatctcac ttattattta aaatgctaat tctcacttag
     1741 aatattactc cttattattt aaaacactat ctatcactta gaatattact tcttatctaa
     1801 attattattt attataaata taaacaatat taatttaaaa agttattcct aatttaaata
     1861 atactattat cttcatcatt aaacctaaac ggcaacttta gacttattag ctaaagccaa
     1921 tattagacct aaagctatag atgcaaatgc agaagcaatt actctatact cagcagggaa
     1981 taagaataca attgtatgac atggtatcca gaaaaatggt atagttttta aaacactaaa
     2041 accaaggtaa gcattccagt ccacaatatt taccacatct ttaagtttta ccttaatccc
     2101 ctttccaacc tcatattttt tatctataaa acagtccata accttatgga ataaaaatac
     2161 agttggtgac attaggatat tcatagttgc actcttaaat agagcatttg caaaagctat
     2221 tccctcaaat ggaagcatac catccacttg tgcttgacct acacctgatg agtagattga
     2281 cattccatat gtggtaacta cacctattat tccccaaact attgctctat aaaatactcc
     2341 tactggtatt ttccaatcct tatacaaaat tctagcacca agtaaatctc ccattgtagc
     2401 taatacagca aactttaaaa aacctccaat atatggatgt gcacctgttg cttccataaa
     2461 tacttctcta ctacttggta ttgctaagac ggcaataata caaataagaa ctccactcca
     2521 tataaaatcg ccttttttca tatttataaa tactatttaa tatataaata gttctccttt
     2581 ctattaaaga tttatttaat ttacaatcta tctacaatta tttattaatc acttattatt
     2641 agatattcat tttccatatt taaatttatt ctatagcttt atctattttt ttctgatatt
     2701 cttatccacc attatctata tatttatttt acgattactc tttcactcta accctttaat
     2761 tatctatttt ttcttactat tcttatcaac catcatccat atatttattt tacgattact
     2821 ctttcactct aactctttaa ttatctattt ttttcttact attcttatca accatcaccc
     2881 atatatttat tttacgatta ctctttcacc ctaacccttt aattatctat ttttttctga
     2941 tattcttatc taccattgtc tatatattta tcttatgatt actctttcac cctaaccctt
     3001 taactatcta ttttttctga tgttcttatc tactattatc tgtatattta ttttatgatt
     3061 actcttttaa cctaacctcc tatactttat aataatatgt ttttaacctt tttcattatt
     3121 cccaatatgc tttataacta caaatgctct ccccccttaa aaataataat aaaattaatc
     3181 aacaaattta taatcatatt attactttat gtattaagca attactatgc caataaattt
     3241 tgattttttt aaacaaatct taaaaatacc aaaatgctaa ttttctttaa cttttaatga
     3301 atctaatcga taacatttaa tttttgttat atttttcaga atgtttcaat tattattttt
     3361 aagaaaaaaa aataatgtta acatttctta acatacatta aaaaatgtta acattataaa
     3421 ttgggtttaa tacttttact ttgactcgat ttacttttat tctacttctt actttatttt
     3481 taacttagac tttattttta acttaaactt tattttttta ttacttattt gagtcttatt
     3541 tattttattc tcactccata tgttcaatta catttgtcta agttttttga tacttttatt
     3601 acataatttt aacaagctta agtgttattt gggtagtgca gttgataatt atttaaataa
     3661 tcatcaactg catatttgta tcattatagt agcttaatgt atttaatcat acttacttag
     3721 gattttgaaa tatcttatag attatattta acattagtca attgctgcat aattgtaagc
     3781 aattgaaatt tgagacccat cttcattttt tatagtgata ggcttcataa tttttaatcc
     3841 ttcagttttt atcttttcta attcttgttc cattttctta attgtttcct caagatcttt
     3901 ttgacttaca tctttgactg cattagggtc tttagcatag tcttttaatt cttgaatatg
     3961 tttagcgtac tcttcataag tataatgctc aacaccatac ttttccaatt cagtagggtc
     4021 agttattcct gattttatta gagattcaag tttaggtaaa gcaatattag atgaatcatt
     4081 tccattcgca aatactgctc cagttcctcc tagtaccatt gcagatacca tcattgcact
     4141 tataagggat ttttttattt tcataaccaa tcttcctttc ttagagattt atttgggtag
     4201 tgtagtttaa aatgactatt gcaatagtta ttaactacac tcttagacta tcatagtagt
     4261 tatatttatg tcaagccttt ttaaaaaact tttcaataaa aaatactatc tcaaaacaaa
     4321 attaattatt ttgagatagt attttaattc taaatctttt atattactaa gatttatggt
     4381 tactctatcc atgttctata ttgttcctta ttaactgcta acaaattccc ttttcttgat
     4441 atgacaaagc gattatcatt atagttactt cgctcaattg aaacttctcc tgaatcagta
     4501 taaagtacat ccttactatt actagattta tttacatacg tttcttcact attctcagaa
     4561 ttagttactt tgctcgtaga tgattcttca tgctctctaa attcaattac agatactttt
     4621 gccgtactaa aatgagtctc tccatcactt ttagctttac taacaagaat atcagattct
     4681 aattttggag aatataaatc agttgattta ctacttattg aaaatccaat tttatcactt
     4741 tgttcatttt ttgttaaacc aaaagattca acctcattaa tctctttatt ttctgttgca
     4801 aaaacaagac ctgtaccaca aactaataca gttgaaacta aaaaagtaac ataagttgat
     4861 tttttaaatt tcataatatt aactattctc ttttctattt ttgtctttcc aaacccacta
     4921 catattgtca ttgaaaatgg attattctcc tctgctagac ttattagtgt ggttgcgtaa
     4981 tcagccctat attcttcacc aagtatactc ataacctttt catcacaact aaattctatg
     5041 tctttattta gtaaaaaaaa catcacccaa actaaaggat taaaccaatg gacaacaaga
     5101 gctacaagca taaataactt taacagatta tcaaagctat taatatgaac catttcatga
     5161 gttaaaacaa agtctaaagt ttgattatta gcataattca tttgctttgg taaaataatt
     5221 cgtggtttta aaataccaga tgtaagtggt gtcctagtaa attcagatat gcaaacaata
     5281 aatcttctaa aacggctatt cttacacatc cactttttaa tgaactcatg attttttata
     5341 tgaattgaag ttttaaaaag attaattatt ttaaaatatc taaagcataa aagtatgcta
     5401 aacataatag taactataaa agacaaaatg cccaacattt ccaacatcat aatattttca
     5461 atactagaca ttatttcaaa ttcgtcagaa ttgtttttac ctgcagagtc actagctttc
     5521 ttaagagtat aattttctgt tgatgtatta cccaattcta catcaaaagt cgattcaatt
     5581 atactataaa tactaaaacc agaatttaaa cttactggta ttagaagttt aaataaaaca
     5641 atcaaccaca ataatacaaa taccttttta ggtaattttt taaataaaca tgctcttata
     5701 aaaataacta ttaaaagtaa tactccagaa gtaaaactca tttctaacaa tttaatgaac
     5761 ataaatctta cctcacttta atttttcaac aatttgttta agttttgtaa tctcatcatt
     5821 tgaaagattt tctttattaa taaaagctga aagaaatttt tctgaagaac ctccaaacat
     5881 tttattaatt aaagtttctg tttcctgttt ttgtacttcc tctttgctta ttaaagcttt
     5941 acaaacaaaa ttcggctcaa ttctctctat tgcacctttt gacactaatt tttttataac
     6001 ggtatacgta gtgtttctat tccatcctgt ttcttctttt aaaattttta ctaactctcc
     6061 agccttgctt tctcctctct cccatagaac ttccattact ttaagttctg aatcaaataa
     6121 ttttatctcc atataaaatc gctcctcaaa tatagttatc acaatagtca ttactataat
     6181 aaaaaactat catagtagtc tatactatgt caatcattta ttttttaata ataaaaactg
     6241 tcttttaaaa aaattcttat aaaagacagt tttatcaatg ttatacttta atgtttaatt
     6301 aaagccatat tttatttaaa tgtataacca tctatcttag tacatagtat aagatttttc
     6361 tttatctaat agtatatcct ctacatctgg attttctttt actaattgtc tataaaaatt
     6421 acaattagta acacctacat atggaattaa ccatagtaga cctattccaa gagtagctat
     6481 tgaaagtata taccatccta taaaactaaa gtttagtata aataatttaa gtacatttcc
     6541 tttcatcata tctaaactag cacccattga tttaaataca cccatattat ccttgtcttc
     6601 acaaattaag aaagtagctt gagaaacata taatagcaat atataaactg gtaacataag
     6661 aacaagtatt ataagaacta gagttattcc agtagaaata atagtcccta ttccaacatt
     6721 atcacttgat aaatattcaa ttgctccact agtaagtaaa actccaaatg ataaaaacgc
     6781 tcctataatt gtgattataa aagaataaat ataaacaata attgattcaa ataaataaaa
     6841 accaaaactt cttaaataaa ttctcccact tacaaagata tctcctatct tgactttttc
     6901 atctttagta attttcaaga acatacatgt gaatcctgct gaaacgcaaa aagctattac
     6961 agccactaca atagtagtca atggctcaaa aggtaagcct acaattgata ttaacactaa
     7021 caatgcaata tatactgcca tagaaattgc acatattccc cagtttccag ataactgagc
     7081 tttagatact ctttttaatt cttgccttga taccataaaa aacacccctt tcattttata
     7141 aaaaactttt aaaaatataa ataacctttt aacgtataat attaatatat catattttcg
     7201 actatataag atacaattat tcaccatgtt ttatcaagat atgccataat tgtattacta
     7261 gttttatata gtcaacatta ccaaatacat aaaataaatt tgactaat
//
LOCUS       DAEPXF010000181         7264 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain HB27
            SAMN13376389-rid12479423.denovo.188, whole genome shotgun sequence.
ACCESSION   DAEPXF010000181 DAEPXF010000000
VERSION     DAEPXF010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13376389
            Sequence Read Archive: SRR10518432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7264)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7264)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001069624.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001069624.1
            Genome Coverage       :: 315x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/15/2021 01:54:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,165
            CDSs (total)                      :: 4,076
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 89
            rRNAs                             :: 10, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 10 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 19 of 69
            Pseudo Genes (incomplete)         :: 44 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 10 of 69
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7264
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13376389-rid12479423.denovo.188"
                     /strain="HB27"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="China: Hebei"
                     /collection_date="2017"
     gene            complement(163..588)
                     /locus_tag="KRL67_003235"
     CDS             complement(163..588)
                     /locus_tag="KRL67_003235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012201185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HBH1524080.1"
                     /translation="MHGELKIKMYSFTMDCKDPYELAKFYAALLNWEIAFYNEEYACV
                     GAPGTNQGTYPGITFQRNPEYKPPVWPEKPEAQQQMAHMDFAVNDLEEAVQYAIRCGA
                     TIAEEQFTDDWRVMIDPAGHPFCLCQMKSIMESSHFSLL"
     gene            complement(746..1201)
                     /locus_tag="KRL67_003236"
     CDS             complement(746..1201)
                     /locus_tag="KRL67_003236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase/bleomycin resistance/dioxygenase
                     family protein"
                     /protein_id="HBH1524081.1"
                     /translation="MKFGGVLLIVKDINKSKKFYEDVMEQKITLDLGEHVSFENGFGL
                     QSNYKAIIGYELPIYQQSNNFQLYFEVDDIKQWESKINRIENIDFLHYIKEYPWGQRT
                     FRFYDFDKNIIEISESMESVIKRLLKQGLALGEISKRTMYPVEFIIQFQ"
     gene            complement(1239..2072)
                     /locus_tag="KRL67_003237"
     CDS             complement(1239..2072)
                     /locus_tag="KRL67_003237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HBH1524082.1"
                     /translation="MLKKESIRFINKNRSLLVCEDNFVYLLPCIQLRNWISNFTISFP
                     NQTIISDNYTIIPHGSVTLVFFYDATGLHSLLFGPTTKPKTVGSIANRCDVILIIEFQ
                     PAGFFPLIGIQQSELIDKVVPFSIINTSLDLEIKKIFNESLSIDKLILKLEELLISNI
                     KIEYSYEFILAIQLIIQKSGNISSQEISKKVFYSSRHLSRLFNQYLGLSMKSFSRLVR
                     INKSIKLLNNNKTSLMSICNELGFYDIPHFIKDFKIVCGITPQKYRANMSDFYSEIAK
                     F"
     gene            complement(2346..3032)
                     /locus_tag="KRL67_003238"
     CDS             complement(2346..3032)
                     /locus_tag="KRL67_003238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBH1524083.1"
                     /translation="MKITHNSLAVEAKNIIKEYKIGNTTTRVLKEVSLQVMKGEFVSI
                     MGQSGSGKSTLLYILGGLDTPTSGKVYMNGADISHFNDEKMSIIRRRNIGFVFQFYNL
                     IPNLNVEENIMLPLLLDGKNLKDYKNQLDEILYIVGLTDRRKHTPRELSGGQQQRVAI
                     ARALIGKPEILFADEPTGNLDSKTGIEIIDLLDKINRDNGQTIIMVTHSPEAAKSSSR
                     TITVSDGLIV"
     gene            complement(3047..5524)
                     /locus_tag="KRL67_003239"
     CDS             complement(3047..5524)
                     /locus_tag="KRL67_003239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HBH1524084.1"
                     /translation="MKIILKYIFTNIKERKIRTAVMLLSIVLSTVLLFISFSIGLSYE
                     SVQRKMAKGMSGTATISVQSKNPDILTNLEDIPDLNAIKSKVGVLESSAIYNKGGYYE
                     EFSLIAADLSQLNKINKPRLENGDSITDFSGDKIILPNRFTSKYKIKKGDSITLQIYG
                     KSYTFQVSDIASYDTVFLRHTRGVNALLPKETLSKIINKGSGYTRVLIESEEEMTENL
                     VNKLSEELSTEKYTVSNTINETKIISDARQKTMPFFLISFFALTLSIFIIYSSYKVIT
                     LERLPFIGTFRSIGANEKTVTHILMLESILYGSIGGLIAIPIGVVVLNLMLHGLGNSL
                     EQGISIPTVISPIGVIISVIVAIIVSSFSAYIPVKKASHLPIKNIVLGTVEEKNVSNR
                     SILFIGSIMFILSVLLPRISPENTLYLAGGFSLLGLIVATIVLIPLITDIISIVFEFV
                     YKNILGNEGKLAARNMKNNKNIIQNITLLFISISAVIAISVVGNFVKTYITDVFRDAE
                     LQGFADGKMNEEFIEDVRHMDGIKNILPLYVMNNEISGNGVTLSRLEGTDNIELYNSM
                     FAINYTNFEIKKQVTEAFKDKRSVILNEDTLKKVGLSIGDTITLSKDKYDFSYKIVGS
                     FKSRANDVEAVIPSHYAVNDFDKTNYGFLVYTAVNPDAVMIQIRDLFGDTYNWSRTVE
                     EFNNDSLNTISSFLSPMNKMTYFIFLLATVGIINNLLINYIQKRRSIAMYKSIGLSNK
                     QNIKVTLIEGFTSGLLGAVIGIVISILEIQTIFIVAGPKISMKPDLDFKTFIIVGLLG
                     IIVTLIGSIVPIIKGKKMKLIEEIKFE"
     gene            complement(5744..7156)
                     /locus_tag="KRL67_003240"
     CDS             complement(5744..7156)
                     /locus_tag="KRL67_003240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBH1524085.1"
                     /translation="MHKPSYKTVFHSYIIFFMIFICLILTVIGIFLSLITIQKPNGEF
                     IRSDWPKKFTENFNEQIVFIDNKPQVKQSGLEKLRENQLWLQILDSSGNRVYGFLEPK
                     EHKDYYSNSELLTLANQNNSSNGTVYFNTIKNNKNDYIYIIYFPLNISKVNMFFDGSK
                     FVGGKSIVFSIIGIIFLIILVSGIAYGYWVTKCISNITSAVSDISKRSYLPVNIKGVF
                     TDIYDSLNILNEEIHRSDEIQNKTDIMREEWISNITHDLKTPLSPIKGYAELLTDSST
                     NYSPEKVYKYASIMLKNIEYTNTLINDLKLTYQLKNGIIPLNKNNYDLVRLLKELSID
                     ILNYPEYESNIVEFKSNIDNINFDFDYTLLKRAFSNLIVNAFTYGDKNTKVCVHITHI
                     TLNERIEIIISDNGKGMSREEASNLFNRYYRGSNTKQKIEGTGLGLAITKQIIEVHGG
                     NISVESILGLGSSFYIIFPI"
BASE COUNT         2541 a         1146 c          791 g         2786 t
ORIGIN      
        1 tttaaaatat ctaagaaatt attcaataaa aaaatactat ttttttaaat tatcattatt
       61 gataaagctc tactatttag atttttagtg tattcataat aagtaaaagt acaacagtat
      121 atactgttgt acttttttgt aattattaaa caatattttg gtctataaca atgaaaaatg
      181 gctactttcc ataattgatt tcatctgaca taagcaaaaa ggatgtccag ctgggtcaat
      241 cataactctc cagtcatcag taaattgctc ctctgcgatt gtagctccac aacgaattgc
      301 atattgaact gcttcttcta aatcattaac agcgaaatcc atatgagcca tttgctgttg
      361 agcttcaggt ttttctggcc acacaggcgg tttatactca ggatttcgtt gaaatgttat
      421 accaggatac gttccctgat ttgttcctgg agcaccaaca catgcatatt cttcattata
      481 aaatgctatt tcccaattga gtaacgctgc ataaaatttt gctaattcat aggggtcttt
      541 gcaatccatc gtaaatgagt acattttgat ttttagttcg ccatgcataa cgtaattacc
      601 tccaaatata aatttcttcg tcttttcatt tttaagctaa attcaaacat taagtatgta
      661 atttattcca agtgtttttc tattatgtca caagcctttg tttttgcaat ataaagaaaa
      721 aattatatat tttaatattt tataattatt gaaattgtat aataaattca actggataca
      781 tcgtacgttt tgaaatttct cctaaagcta agccttgttt taaaagcctt tttatgacac
      841 tttccatact ttctgaaatt tctataatat ttttatcaaa atcataaaac ctaaaagttc
      901 tttgacccca tgggtattcc tttatataat gtaaaaaatc aatattttct attctattta
      961 tcttactttc ccattgtttg atatcatcaa cctcaaaata caactgaaag ttatttgatt
     1021 gttgatatat tggtaactca tatccaatta tagctttata attactttgt aatccaaatc
     1081 cattttcaaa tgatacatgt tctcctaaat ctaatgtgat tttttgctcc ataacatctt
     1141 cataaaactt ttttgattta tttatgtctt tgacaattag caatactcct ccaaatttca
     1201 tcttatcact atcctttctt tgttataagt attataactt aaaattttgc aatctcactg
     1261 taaaaatcag acatattagc tcggtatttt tgaggagtta ttccacagac aattttaaaa
     1321 tctttaataa aatgtggtat atcatagaat cctaattcat tacatataga catcaatgaa
     1381 gttttattat tgtttagaag ttttatagat ttattaatac gtaccaaacg agaaaatgac
     1441 ttcatactca accctaggta ctgattaaaa agtctgctta aatgcctaga actataaaat
     1501 acttttttag aaatttcttg tgaagatatg tttccagatt tttgaataat caactgaatt
     1561 gccaaaataa actcatatga gtattcaatt ttaatattag aaatcaataa ttcttccaac
     1621 tttaaaatca atttatctat agataaagat tcattaaata tttttttaat ttctaaatct
     1681 aatgaagtat taattataga aaatggaaca actttatcaa taagttcgct ttgctgtata
     1741 cctataagcg ggaaaaaacc agcaggttga aattctataa ttagtataac atcacatcta
     1801 ttagcaatac ttcctactgt ttttggttta gtagttggac caaataatag actatgtaat
     1861 cctgtggcat cataaaaaaa caccaaagtt acacttccat gaggaataat cgtgtaatta
     1921 tcagaaatta ttgtttgatt tggaaacgat atagtaaaat tcgatatcca atttcttaat
     1981 tgtatacaag gcaagagata aacaaaattg tcttcacaaa ctagaagcga cctattttta
     2041 tttataaaac gaatactttc tttttttaac atatattata tcctgtccta tcgtgaatat
     2101 ttattaaaat aagaagaatc catactatct ctacgtatca ttaactagtt ttaataataa
     2161 agatagaatg aatactcatt agatatttaa taaacaaata aaattaaaat aatcaataaa
     2221 aaatatagta ctcttatctg ttattttaat attatagcac atttataata tttaatttat
     2281 atccacttta catatgtcta atttatataa ttaaaaatga ttatctcaaa tacaataatt
     2341 agtccttata caattaaacc gtctgaaact gtaatagtac gactactact tttggctgct
     2401 tctggtgagt gtgttaccat aattatagtt tgtccattgt ccctgtttat tttatccagt
     2461 aaatctatta tttctatccc tgttttacta tctaaatttc cagtaggctc atcagcaaat
     2521 aaaatctctg gttttccaat taaagctcga gctatagcaa cacgttgttg ctgaccacca
     2581 gataactctc gtggtgtatg tttacgtcta tcagttaaac caactatata taagatttca
     2641 tctaattgat ttttataatc ttttaaattt ttaccatcta atagcaaagg aagcattata
     2701 ttttcctcca catttaaatt tggtattaag ttataaaatt gaaatacgaa tcctatattt
     2761 ctacgacgta ttatactcat tttctcatca ttaaaatgtg atatatcagc tccattcata
     2821 tacacttttc cactcgttgg agtatctaaa ccacctaaaa tatataaaag agtactttta
     2881 ccagaaccag attgacccat gatagaaaca aattcacctt tcattacttg taaagacacc
     2941 tcttttaaca ctcgagttgt tgtatttcca attttatatt ctttgataat attttttgct
     3001 tctacagcta aactattatg tgtaattttc atattaacct ccataattat tcaaacttaa
     3061 tttcttctat cagtttcatt ttcttccctt ttataatagg cactatcgaa cctattaaag
     3121 ttactataat tcccaataac ccaacaataa taaatgtttt aaaatctaaa tctggtttca
     3181 ttgaaatttt tggtcctgca actataaaga tagtttgaat ctcaagtata gatattacaa
     3241 taccaataac agctccaagt aaacctgaag taaacccttc aatcaatgtc acttttatat
     3301 tttgtttatt acttagtcct atagatttat acatagctat agaacgtctt ttttgaatat
     3361 aattaatcag tagattatta ataataccta ctgtagcaag aagaaaaatg aaataggtca
     3421 tcttattcat aggagacaaa aaactactta tagtatttaa agaatcatta ttaaattctt
     3481 caactgtacg actccaatta tatgtatctc caaataaatc ccttatctga atcataacag
     3541 catctggatt gactgctgta tatactaaaa aaccataatt agttttatca aaatcattaa
     3601 ctgcataatg tgatggtata acagcttcta catcatttgc acgagattta aaactaccta
     3661 ctattttata agaaaaatca tacttatctt tagatagagt tattgtatct cctatagata
     3721 accctacttt ttttagagta tcttcattca aaataacaga acgtttatcc ttaaaagctt
     3781 ctgttacttg tttttttatc tcaaaatttg tataattgat tgcgaacata gagttatata
     3841 gttctatatt atctgtaccc tctagtcttg ataatgttac accattacca gaaatctcat
     3901 tattcataac ataaagaggt aatatatttt taattccatc catatgtcta acatcttcaa
     3961 taaactcttc attcatcttt ccatctgcaa aaccttgtaa ttctgcgtca cgaaacacat
     4021 ctgttatata agttttgaca aagttaccaa caacgcttat tgcaataact gcagaaatac
     4081 ttataaatag taaagtgata ttctgtatta tatttttgtt atttttcata tttctagcag
     4141 ctaatttacc ttcattccct aaaatatttt tataaacaaa ctcaaaaact atagatatga
     4201 tatcagtaat aagaggaatc aacacaatag tagctacaat taatcctagt aatgaaaatc
     4261 cgccagctag atacaatgta ttttcagggg atattcttgg aagtaaaaca gataaaataa
     4321 acattatact tcctataaaa agtatagaac gatttgaaac attcttttct tctactgttc
     4381 ctagtacaat attttttatt ggcaaatggc tagctttttt aacaggtata tatgcactaa
     4441 atgaagaaac gattattgct actataacag atataatcac tccaatgggt gatattactg
     4501 ttggaattga tattccttgt tcgagagaat ttcctaaccc atgaagcatt aaatttaaaa
     4561 caactacacc aataggaata gcaattaatc ctcctatact tccgtataat atactttcaa
     4621 gcatcaatat atgagttaca gttttttcat tggcaccaat acttctaaat gttccaataa
     4681 aaggaagacg ttctaatgta ataaccttat aactgctgta tatgataaat atactaaggg
     4741 ttaatgcaaa aaaacttatt aaaaagaaag gcattgtctt ttgtctagca tctgaaatta
     4801 tcttagtttc atttatagta tttgatactg tatatttttc agtagaaagt tcttcggata
     4861 atttatttac tagattttct gtcatttctt cttctgactc tataagaact cttgtatatc
     4921 cactgccttt atttataatt tttgatagag tctctttagg tagcagtgca ttcacacctc
     4981 ttgtatgcct caaaaatact gtatcataag atgcaatatc actaacttga aaagtataag
     5041 actttccata aatctgtaaa gtaatagaat cacctttttt tattttatat tttgatgtaa
     5101 aacggtttgg aagaataatt ttatcaccag aaaaatcagt aatactatcg ccattttcta
     5161 atcgtggctt gttgatttta tttagttgac ttaagtctgc tgctataaga ctaaattctt
     5221 catagtatcc ccctttatta taaatagcag agctttccaa tacaccaacc tttgatttta
     5281 tagcatttaa atcaggtatg tcttctaaat tggttaatat atctggattt ttactttgga
     5341 cagatatagt cgctgtacca gacatacctt ttgccatttt tctttgtaca ctttcataag
     5401 ataatccaat tgagaatgaa ataaaaagta aaactgttga taaaactatg gacaataaca
     5461 taactgctgt tctaattttt cgttctttaa tatttgtgaa aatatacttt aatataattt
     5521 tcaaatttac accttcttcc tttatctaag atatttcagt ataatatttt tatactttaa
     5581 tccaaattga atctctaata gactcctcca tacttttatc attctattta taatattttt
     5641 aatcaaatca cctccttaaa taaaaaagat tgataaacta ctatataaat ataataatct
     5701 atcaacctta atatattttt tatttgacct taattaacct taattaaata ggaaagatga
     5761 tataaaatga actacccaat ccaagtatac tttcaacaga tatattccct ccatgaactt
     5821 caataatttg ctttgttatt gctagtccta aaccagttcc ttctattttt tgttttgtat
     5881 tgcttcctct ataataccga ttaaacaaat ttgatgcctc ttctcttgac atcccctttc
     5941 cattatcaga tataatgatt tcaattcttt catttaatgt tatatgtgtt atatgtacac
     6001 aaactttagt atttttatct ccatatgtaa aagcattgac aataagatta ctaaaagctc
     6061 gttttaatag tgtataatca aaatcaaagt taatattatc tatattactc ttaaattcaa
     6121 ctatatttga ttcatattct ggataattta aaatatcaat ggataattct ttcaagagtc
     6181 ttactaaatc atagttattt ttatttaaag ggattattcc attttttaat tgataggtta
     6241 attttaaatc atttataagt gtatttgtat attctatgtt ctttaacatg attgaagcat
     6301 atttatatac tttttcaggt gaataattag tactactatc tgtaagaagc tctgcatacc
     6361 cttttattgg tgaaagtgga gtttttaaat catgagttat atttgaaatc cactcttctc
     6421 tcataatatc agttttattt tgtatctcat cacttctatg aatttcttca tttaaaatat
     6481 ttaaactatc atatatatca gtaaaaacac ctttaatatt aactggaaga tagcttcgtt
     6541 ttgaaatatc actaacagca gatgtaatat ttgatatgca ctttgttacc cagtaaccat
     6601 aagcaattcc agaaaccaaa attattaaaa atataatccc tatgattgaa aatacaattg
     6661 acttaccacc tacaaattta cttccatcaa aaaacatatt cactttagat atatttaatg
     6721 gaaaatatat aatataaata tagtcatttt tattattttt aattgtatta aagtatacag
     6781 ttccattact tgaattattt tggttagcta atgttaataa ttcagaatta gaataataat
     6841 ctttatgctc ttttggttct aaaaacccat aaactctatt tccactagaa tctaaaattt
     6901 gcaaccacaa ttgattttct cgaagctttt ctaatccaga ctgttttact tgtggcttat
     6961 tatctataaa tacgatttgt tcattaaagt tttctgtaaa ttttttcggc cagtcactac
     7021 gaataaattc cccatttggt ttctgaatag taataagtga taagaaaata cctattactg
     7081 tcaggattaa gcaaataaaa atcataaaaa aaataatgta tgaatgaaat actgttttat
     7141 aactaggttt atgcatttaa tcaactcttt tcttaaactt ttattttagt ttgattgttg
     7201 cttatataaa atagacaata attattgaca tgtaaaaatt catttaaaaa tacaactgtc
     7261 taca
//
LOCUS       DAEPXF010000182         7103 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain HB27
            SAMN13376389-rid12479423.denovo.189, whole genome shotgun sequence.
ACCESSION   DAEPXF010000182 DAEPXF010000000
VERSION     DAEPXF010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13376389
            Sequence Read Archive: SRR10518432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7103)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7103)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001069624.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001069624.1
            Genome Coverage       :: 315x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/15/2021 01:54:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,165
            CDSs (total)                      :: 4,076
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 89
            rRNAs                             :: 10, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 10 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 19 of 69
            Pseudo Genes (incomplete)         :: 44 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 10 of 69
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7103
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13376389-rid12479423.denovo.189"
                     /strain="HB27"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="China: Hebei"
                     /collection_date="2017"
     regulatory      57..159
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            253..1629
                     /locus_tag="KRL67_003241"
     CDS             253..1629
                     /locus_tag="KRL67_003241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HBH1524086.1"
                     /translation="MQTTNSSTMQKLFPILTNKNVDMKKEIIAGVTTFLTMAYIIAVN
                     PNILSETGMPAGALVTGTCLAAAFGCILMGVVANLPFALASGMGLNAFFAYTVVLQMG
                     VPWEVALTAVFVEGIIFIVLSVSGVREAVVNAIPKNMKLAVTGGIGIFIALIGLVNSG
                     IVIGDQATLIKMGRFTPAVIITCVGLIIIAVLDKKRVKGSILFGIVVSSLLAWAFAFM
                     NPEYAQKLGIYLPGGLFKFESLAPIAGKIDLGYVLHPTNIGGFLVVVCTFLFVDFFDT
                     VGTLVGVCSKANMLDEKGNVPNVGRALLTDAVATTVGAGLGVSTVTTYVESSTGVIAG
                     GRTGWTAITVGILFLAAMFFSPVFIAIPSCATAPALIYVGYLMLGTVKDIEFDNITEG
                     VPAFVTIACMALTYSIGDGLTLGILTYVFVNIFYNIFGAKKAEDKKHVSVVMIVLAIL
                     FIIKLWLL"
     gene            complement(1742..2887)
                     /locus_tag="KRL67_003242"
     CDS             complement(1742..2887)
                     /locus_tag="KRL67_003242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HBH1524087.1"
                     /translation="MNTFEQLKISSTLIEGLKKQDITSPTEVQSLVIGKIIQNKDLLI
                     NSQTGTGKTLAYLLPIFEKIDTSKRETQALILAPTHELVMQITNQVELLAKNAELSVT
                     SLALIGEVNIQKQIKNIKAVKPHIVIGSCGRVLDLIKQKKLKSHNIKTIVLDEVDNLL
                     NGKNITCIEDIIRTTLRDRQIIGCSASLTDSTIKICDKLMKEFEIIKTKEKSQINPNI
                     NHSYLLGEIRDKFTFLRKALAATNPKKAIVFVNNEKNIEVLVSKLNYHNYKAIGIFGN
                     MEKEDRKNAINKFKLGKAKILITTDLSARGLDIVDVSHVFNLDFPKSKNEYLHRCGRT
                     ARGNRSGNTISIITKKELDIIKDLQKEFNIVITPKTLQNGELIDIIK"
     gene            3139..3846
                     /locus_tag="KRL67_003243"
     CDS             3139..3846
                     /locus_tag="KRL67_003243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBH1524088.1"
                     /translation="MLKVINVSKSYKNKKVVSSVSFDVNDGELFGFIGHNGAGKTTTI
                     KAIVGIHDFEEGEVLINSKSIKKQPVECKKEMAYIPDNPDLYESLTGIQYLNFISDIF
                     EVAKDKREELIKYYSNKFELNKALGDLISSYSHGMKQKLAIISALIHSPKILILDEPF
                     VGLDPKASFILKEIMREFCDKGGCIFFSTHVLEVAEKICDKIAIIKDGNIIAYGTTEE
                     VKGSNSLESIFMELIEK"
     gene            3893..5488
                     /locus_tag="KRL67_003244"
     CDS             3893..5488
                     /locus_tag="KRL67_003244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBH1524089.1"
                     /translation="MSNLSILIKTNIINEFKLNALKKADGREKQKIMGMMLSVVIIAG
                     LLIFYVTSLSLSLVDILKQINQMEMLLIFGFGLSTLTTIVTSLYKTSSYLFQAKDLDL
                     LTSLPIKESTVLASKILMLVGANYLFSSICMIIPAVVYFFNSDVSITYFPYLILLFLA
                     LPLLPIVASSIIFMFIGQISSRIKYKNLVLIIGSIILMLLYTLLMTRLNSMSVEMLKN
                     SVSILETIRKIYPPIYYFVDALKTGSVVSFLAFIAISIIPFFMFVTLFAKKFKDINAK
                     MSEGYKSKTYKFKSQKTSRPSKALLKKEFKRYTSSFIYFLNSSAGLLFLIVGTILILL
                     FGADKVGELLKLNIDFNFIKIQLLGVFFFIIVMNCTTYCSISLEGKNLWILKSSPIDE
                     REIFWSKILMNFLLNAPLSILCLILVSIRLDFEIQFIIVSICLIVSFSMFVAVMGLLS
                     NLLYPNLDWKNEVAVVKRSASMIITMLVSLAYVIILGGIYVLLGISFLNIYIILASIL
                     TLILDFMIWRIISTKGVKIFKNL"
     gene            5591..6673
                     /locus_tag="KRL67_003245"
     CDS             5591..6673
                     /locus_tag="KRL67_003245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase-like domain-containing protein"
                     /protein_id="HBH1524090.1"
                     /translation="MRKLSKREKDFIQYGFLLFLICITTYLVLTTLDIKLIPKIIRLV
                     NKTYIFLGILTIIVYMIIESIVTHLIINAVQKTKVKFIGIKMATMGFYYNLVTPFASG
                     SQPMQIYALTKYDVSLSKSVAIVTNKTVVFQSTVTVFCGILILLNKSLLMEQVHSVKV
                     LITFGMIMNVSMLLGGLLIVFSPKQVKMIAEVLINFLSKFKTFKFLDSKRDRINHYID
                     DYNYSIKIFIKNVRILVLSILLTVLQLIAYFSIVYCVYKASNLRGVSYVHIMTLQVFL
                     YMAISPIPTPGNVGANEVTFFTMFSNIIPKELLGYSVLLYSGFVYYFILIVSGIFTFG
                     THYTLNSWKKENKVNSNLDKVIEKLN"
     gene            6822..6983
                     /locus_tag="KRL67_003246"
     CDS             6822..6983
                     /locus_tag="KRL67_003246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III"
                     /protein_id="HBH1524091.1"
                     /translation="MKQDKKNEKQSEISKIKQSNDNKLEIAQELHVSKNIGNMLGYRI
                     DKKKMNRPS"
BASE COUNT         2507 a          782 c         1104 g         2710 t
ORIGIN      
        1 tacgtatata ttgacaaaat taattatata aattaaaata atattcgtaa agaaaaattc
       61 atgttttgta ctgtataaca tataattttg acaatatggg tcataagttt ctaccggaat
      121 accgtaaata ttctgactat gtatactatt atttagttaa ttaaatattt aattaactga
      181 attatgaagg atacggctca gcttatcaac tactgtgtcg tttttttgta aaatttaata
      241 ggaggtgttg ttatgcaaac aacaaacagt agtactatgc agaaactttt tccaattttg
      301 actaataaaa atgtcgacat gaagaaggaa ataattgcag gagtaactac ttttttaact
      361 atggcttata taatagctgt aaatccaaat attttatctg agacaggcat gcctgcaggt
      421 gcacttgtta caggtacttg tttagcagca gcatttggat gtattttaat gggtgtagtt
      481 gctaaccttc cttttgcttt agcatcaggt atgggtctta atgcattttt tgcatataca
      541 gtagttttac aaatgggtgt tccttgggaa gtagctctta cagcagtatt tgtagaaggt
      601 attattttta tagtattatc agtaagtgga gttcgtgaag ctgttgtaaa tgcgatacca
      661 aaaaatatga aattagctgt tacaggtggt ataggaatat ttattgcact tataggtcta
      721 gttaacagtg gaattgttat tggagaccaa gctactctta tcaaaatggg tagatttaca
      781 ccagcagtaa taataacttg tgtaggatta ataattattg ctgtattaga taaaaaaaga
      841 gttaaaggtt ctatactttt tggaatagta gtgagttcat tacttgcttg ggcatttgct
      901 tttatgaatc cagagtatgc acaaaaatta ggtatatatt tacctggtgg attatttaaa
      961 ttcgaaagtt tagcaccaat agcaggtaaa atagatttag gatatgtact acatccaaca
     1021 aatataggtg gttttttagt agtagtttgt acatttttat ttgttgattt ttttgataca
     1081 gtaggtacat tagttggtgt ttgttctaaa gcaaacatgt tagatgaaaa agggaatgtt
     1141 cctaatgtag gtagagcctt acttacagat gcggttgcaa ctacagttgg tgcaggactt
     1201 ggagtatcta cagttacaac ttatgttgag agttcaacag gagttatcgc aggtggaaga
     1261 actggatgga cagcaataac agttggaatt ttattcttag ctgcaatgtt tttctctcct
     1321 gtatttatag caattccatc atgtgctaca gcaccagctt taatatatgt tggatattta
     1381 atgcttggta cagttaagga tatagagttt gataatataa ctgaaggtgt tccagctttt
     1441 gttactattg cttgtatggc attaacatat agtataggtg atggtttaac tttaggtata
     1501 ttaacttatg ttttcgtaaa tatattttat aatatatttg gagctaaaaa ggcagaagat
     1561 aaaaaacatg tttctgtagt aatgattgtt ctggcaatat tatttataat aaaactttgg
     1621 ctactataat ggtattattt aaaattttaa tttaatataa aataaagggc tgtaatttat
     1681 tatcaattgt gatgataaac tacagctctt ttgtatttgt tgtattttcg tatttattgt
     1741 attatttaat aatgtctatt agttctccat tttgtaaagt tttaggtgta attactatat
     1801 tgaactcttt ttgcaaatct tttataatat ctaattcttt ttttgttatt attgagattg
     1861 tgttaccact tcgatttcct ctagcagttc gaccacatct atgcaggtat tcatttttac
     1921 tttttggaaa atctaagtta aaaacatgac ttacatccac aatatctagt ccacgagcag
     1981 ataaatcagt tgtaattaaa atctttgctt taccaagttt aaatttattt attgcattct
     2041 ttctatcttc tttttccatg tttccaaaaa ttcctattgc tttgtaatta tgataattta
     2101 gttttgatac taggacttct atattttttt cattatttac aaatacaata gctttttttg
     2161 gatttgtagc tgctaatgcc tttctaagaa aagtaaattt atcccttatc tcacctaata
     2221 aatatgagtg attgatgtta ggatttattt gtgatttttc ctttgttttt attatttcaa
     2281 actccttcat aagtttatca caaattttta tagtgctatc agtaagactt gcagagcatc
     2341 ctataatttg tctatctctt aacgttgttc ttattatgtc ttctatacaa gttatatttt
     2401 ttccattcaa tagattgtcc acttcatcta agacaatagt ttttatatta tgtgatttta
     2461 gcttcttttg ttttatcaag tctaaaactc ttccacaaga gccaattact atgtgtggtt
     2521 ttactgcttt tatatttttt atttgttttt gtatattgac ctccccaatt aaagctaaag
     2581 aagtaacaga gagttctgca tttttagcca ataactcaac ttggtttgta atctgcatta
     2641 ctaattcatg cgtaggtgca agtataagag cttgtgtttc tctttttgat gtatcaattt
     2701 tttcaaatat aggtaaaagg tacgctagtg tttttcctgt tccagtctgt gaattaatca
     2761 ataaatcctt attttgaatt atcttcccta taacaagtga ctgaacttct gttggagaag
     2821 ttatgtcttg ttttttaagt ccttctatta gagtggaact tatttttagt tgttcaaatg
     2881 tattcatatt ttgctcctta tatttaataa ttatagtcaa ctatttttta ttatacattg
     2941 tttatgtaaa tcgaactata tattctttta taattatgtg taatttgaaa gtaaatatca
     3001 attatattta tacctttcta tttctgataa ctttgcattt tatagatttc taaaataaac
     3061 aaataaaagt tgtaattgtt tgatatttgt tatatattat atataacaaa tatcaaaact
     3121 ataatatgga gggaaattat gttaaaagta atcaatgtat ctaaatcata taaaaataaa
     3181 aaagtagtaa gtagtgtctc atttgatgta aatgatggag agctttttgg gtttatagga
     3241 cataatggag caggtaaaac tactactatt aaagcaattg ttggaataca cgattttgaa
     3301 gagggagagg tactaataaa ttctaaatct ataaaaaaac agcctgttga atgtaaaaag
     3361 gaaatggcat atatacctga taatccagat ttatacgagt cattaactgg tatacaatac
     3421 cttaatttta tatctgacat atttgaagtt gccaaagata aaagagaaga gttaataaaa
     3481 tattattcaa ataagtttga actcaataaa gcattaggag atttaatatc ttcatattcg
     3541 catggtatga aacaaaaatt agctataatt tcggctttga tacacagccc aaaaatctta
     3601 attcttgatg aaccttttgt aggtcttgac ccaaaagctt cctttatatt gaaggaaatt
     3661 atgagggagt tttgtgacaa aggtggttgt atatttttct caacacacgt tttagaagtt
     3721 gctgaaaaaa tttgtgataa gattgccata ataaaagatg gaaatataat tgcttatgga
     3781 actacagaag aagttaaggg aagtaattca cttgaaagta ttttcatgga attaatagag
     3841 aaataaaatg ttttttatac aatatagtgt ctaaagtgag gaatgataaa atatgagcaa
     3901 tctaagtata ttaataaaaa ctaatataat aaatgaattt aaattaaatg cgttaaaaaa
     3961 agctgatggt agagaaaaac aaaaaataat gggaatgatg ctttcagttg ttataatagc
     4021 agggctatta attttttatg taacgagctt aagcctttct ttagttgata ttttaaagca
     4081 aataaatcag atggaaatgc tactgatatt tggatttgga ttatcaacat taacaactat
     4141 tgttacatca ttatataaaa cgtcatcata tctgtttcaa gcaaaagatt tagacctttt
     4201 gactagttta cctataaaag aatctactgt gctagcaagt aagatattaa tgttggttgg
     4261 agcaaactat ttattttctt caatatgtat gattatacca gcagtggttt atttttttaa
     4321 ttctgatgta agtataacgt atttccctta tttgatactg ctatttttag ctttaccatt
     4381 attaccaata gttgcttcat caataatttt tatgtttata gggcagatat catcaagaat
     4441 aaaatacaaa aatttagtat taattatagg tagtattata ttgatgttgc tttatacttt
     4501 acttatgaca agacttaact ctatgtcggt tgagatgtta aagaatagtg tatctatttt
     4561 agagacgatt agaaaaatat atccacctat atattatttt gttgacgctc ttaaaacagg
     4621 aagcgtagtt tcctttttag catttatagc aatttcaata ataccatttt ttatgtttgt
     4681 aacattattt gcaaaaaaat ttaaagatat aaatgctaag atgagtgaag gatataagtc
     4741 aaaaacttac aaatttaaga gccaaaaaac ttcaagacct tcaaaagctc tattaaaaaa
     4801 agaatttaag agatatactt cttcatttat atacttttta aattcatctg caggtttgtt
     4861 atttctaata gtaggtacaa ttttaatact attatttgga gcagataaag tgggtgagtt
     4921 actgaagttg aatatagatt ttaattttat aaaaatacaa ttattgggag tattcttctt
     4981 tataatagta atgaattgta caacatactg ttcaatttct cttgaaggaa aaaatttatg
     5041 gatactgaaa tcttctccaa tagatgaaag agaaatattc tggtcaaaaa tattgatgaa
     5101 ttttttattg aatgcaccac tatctatttt atgtctaata ttagtatcta taagattaga
     5161 ctttgaaatt cagtttatta ttgtatcaat ctgtttgatt gtaagttttt caatgtttgt
     5221 agcagtgatg ggattattat caaatttatt atacccaaac cttgattgga aaaatgaggt
     5281 ggctgtagta aaaagaagtg caagtatgat aattacaatg ttagtatcat tagcttatgt
     5341 aattatactt ggaggtatat atgtattact tggaatttcg tttttaaata tatatattat
     5401 tttggcaagt atacttactt tgatattaga ttttatgata tggagaatta tttctactaa
     5461 aggagtaaag atatttaaaa atttataggt gtatttatgt caaataaaga cttatctttt
     5521 ttagtcttgc aaataatagg ttaagtagta tactatgtat agtgatatga aaaaatttag
     5581 gagaaaataa atgagaaaat tatccaaaag agaaaaagat tttattcaat atggattttt
     5641 attatttttg atatgtatta ctacctattt agtattaact actttagata taaaactaat
     5701 acctaaaatt ataaggctag taaataagac ttatatattt cttggcatat tgacaattat
     5761 agtatatatg ataattgaat ctattgtaac tcatttgata ataaatgcag ttcaaaaaac
     5821 aaaagtgaaa tttattggta ttaaaatggc tacaatggga ttttactata acttagttac
     5881 tccatttgca tctgggagtc aaccaatgca aatatatgca cttacaaaat atgatgtcag
     5941 tcttagtaaa tcagtggcta tagttactaa taaaactgta gtatttcaaa gtacagttac
     6001 cgtattttgt ggaattttaa ttttgctaaa taagtcatta ctgatggagc aagtacattc
     6061 tgtgaaagtc ttgataactt ttggaatgat tatgaatgta agtatgttgt taggtggact
     6121 actaatagta tttagtccaa aacaagtaaa aatgatagca gaagttttga ttaatttttt
     6181 atctaaattt aaaacattta agtttttaga ttcaaaacgt gatagaataa atcattatat
     6241 tgatgattat aattattcta taaaaatatt tattaaaaat gtaagaatct tagttttaag
     6301 tatattactt actgtgttac aattaatcgc atatttcagt atagtttatt gtgtgtataa
     6361 agcttcaaat ttaagaggag tttcatatgt acatataatg acattacagg tctttttata
     6421 tatggctata tcaccaattc caacaccagg aaatgttgga gctaatgagg ttacattttt
     6481 tactatgttc tccaatataa ttccaaaaga attattgggg tactctgtac tcttatatag
     6541 tgggtttgta tactatttta tattaatagt ttcaggtata tttacatttg gaacgcatta
     6601 tacattaaat tcttggaaaa aggaaaataa agtaaattct aatttagata aggttattga
     6661 aaaattaaat taataaaata attagggaaa ttctatatat aaatatggcg ttgaataagc
     6721 tatattgcat tgagatattt cccttttttg attaaaatta tagaaatttg aaataataag
     6781 taataaacag aaagattgat attgttttga aaggagaaac tatgaaacaa gataaaaaaa
     6841 atgaaaaaca aagtgagatt tctaagataa aacaaagtaa tgataataaa cttgaaatag
     6901 ctcaagaatt acatgtaagt aaaaatatag ggaatatgct tggatataga atagataaga
     6961 aaaaaatgaa tagaccaagt taatgaaaat gttgaaaatg gtacatttta aaaaatatat
     7021 ataatgaaat gttttattgt taaatttaaa cttattttaa tgaggaaaaa agtcgaaaaa
     7081 tatctaaaaa gatatggata taa
//
LOCUS       DAEPXF010000183         7102 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain HB27
            SAMN13376389-rid12479423.denovo.190, whole genome shotgun sequence.
ACCESSION   DAEPXF010000183 DAEPXF010000000
VERSION     DAEPXF010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13376389
            Sequence Read Archive: SRR10518432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7102)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7102)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001069624.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001069624.1
            Genome Coverage       :: 315x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/15/2021 01:54:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,165
            CDSs (total)                      :: 4,076
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 89
            rRNAs                             :: 10, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 10 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 19 of 69
            Pseudo Genes (incomplete)         :: 44 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 10 of 69
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7102
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13376389-rid12479423.denovo.190"
                     /strain="HB27"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="China: Hebei"
                     /collection_date="2017"
     gene            15..875
                     /locus_tag="KRL67_003247"
     CDS             15..875
                     /locus_tag="KRL67_003247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HBH1524092.1"
                     /translation="MLLKKDRITFSEILILIVGCILMAISLNLFFNPHAIAAGGITGL
                     GVVLNSLFGVELWIVNLLLNVPLFIFAYKILSKKDCFKTVLGIIFLTIALKLTANMAT
                     LDITNDMYLAIISGSILMGVGQGLIFRINGSTGGTDLMALLLNKYFPTFSIPVLMGIV
                     DCVVVVLSGIVNRQVEIALYSTVALYILVKVSDLLIEGFNYSKSFTIISDLSKDISKK
                     IMEDLDRGATILKGEGAYTGENKNVLLVVVEKKEVVELKKLVKNVDPNAFIIITDIHE
                     ALGNGFKKIE"
     gene            1311..1877
                     /locus_tag="KRL67_003248"
     CDS             1311..1877
                     /locus_tag="KRL67_003248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerD family protein"
                     /protein_id="HBH1524093.1"
                     /translation="MSVSLQKGQRVSLVKDNNPVKNLVVGLGWDMNKLGKKNYDLDAF
                     AIALTNQDKMNARGDLVYFGNLKHPSKAIIHTGDNLTGKGEGDDEQIIVNLEDIPEYV
                     HKIVFGVSIYKAKKRDQDFGQMNNSFIRLIDSNSKQELFKYNLQEDFSGKVTVLAGEI
                     YRRNEEWKFNALGIGQNEELRELINTYK"
     gene            1917..2498
                     /locus_tag="KRL67_003249"
     CDS             1917..2498
                     /locus_tag="KRL67_003249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerD family protein"
                     /protein_id="HBH1524094.1"
                     /translation="MGITLAKGQKVSLTKSNPGLKKVIVGLGWDINKYDGGFDFDLDA
                     SAFLTGTDGKVTNDGDFIFYNNLKHASGAVEYMGDNRTGVGDGDDEQINVDLSKIPQN
                     IAKISFSVTINEAITRRQNFGQVENSYIRIYNEETNEELIKYELGEDFSIETAIVVAE
                     LYRHNGEWKFNALGSGFEDGLAGLCKNFGVNIG"
     gene            complement(2555..3472)
                     /locus_tag="KRL67_003250"
     CDS             complement(2555..3472)
                     /locus_tag="KRL67_003250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBH1524095.1"
                     /translation="MTIRSLEIFVKVAECGKMSEVARNMYITQSSVSQAISEIEKEYG
                     VKLFDRISKKLYLTEAGKKLLGYGRHLLAVNEEMNDCMKHCAKNIRIRVGATVTVGTC
                     VISPIMLELYKVNPFIEPEVFVEDTRLIAKKLLNSELDIAIVEGKIKHPDIVTKSIIN
                     DNLVLICSHKHEFYKRDSIKVSELSNQPLIMRELGSGTRAQLEKQLKELKIPMNIRWS
                     CYNSEAILRAVVDNFGIAVISELLIEDYLKKHLLWACDIEGINLHRTFDIAYHRSKFF
                     TENISAFFDISVEYGKKQARKKSRNVNSL"
     gene            3602..5233
                     /locus_tag="KRL67_003251"
     CDS             3602..5233
                     /locus_tag="KRL67_003251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HBH1524096.1"
                     /translation="MKVLIIGGVAAGTKTAAKIKREMGDNCSVTILNKGDDISYAGCG
                     LPYYVGKIIEDKSSLIVNTPESFSKLTGAEVRCGVEVTSIDRNAKQVTLKTISTGEKE
                     NLSYDKLVIATGADPIKPPIPGIDLPGVFFMRTPNDAIKLRDSIENGIKRAVVIGGGF
                     IGLEIAENLSAMGIRVSVVDMAEHVMPGFDTDFAEYVENHMADKGIMIFTGDQVVGIE
                     GENKVEKLRTKNRAIKTDLVVMSVGIRPNTGFLKDTGLEFAPNNTVIADEYMLTNDKD
                     IYVVGDCAFVKNSLTNKPTWSPMGSSANHEGRICAQNISGKSKTYNGVLGTTVVKLPE
                     LNAGKTGLSKEMAEKEGYNVCSVTIATDDKAHYYPGASNFIIRMVAEKNTRKLLGVQV
                     MGPGAVDKIVDIAATAITLGADLYSLESMDLAYAPPFSTAIHPFVVAVNVLQNKLNGE
                     LDSVLLDEIEDFDSWTKLDVSKAPSIPSLRYIPVGEINGEIEGLAKDEKILLICAKGK
                     NSYMAQNRLRRFGYTNTKVLEGGILFYPNLKVMEE"
     gene            5236..6183
                     /locus_tag="KRL67_003252"
     CDS             5236..6183
                     /locus_tag="KRL67_003252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013378669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(4Fe-4S)-binding protein"
                     /protein_id="HBH1524097.1"
                     /translation="MSNVTAEQKKEVKGRGFLPSRDGEHFAARIITVNGVINASQAKK
                     IAEAAEKFGNGQLAFTTRLTVELPGVKFEDIEALSEFIASENLITGGTGSRVRPVVAC
                     KGTVCVHGLADTQALAAEIHEEFYKNWYDVKLPHKFKIGVGGCPNNCIKPDLNDLGIV
                     GQRVPDYDSELCVGCKKCAVVEVCPVKAAKLTDKGKLEIDSNLCNNCGKCIESCNFDS
                     IEEKESGYKVYIGGKWGKSVRPGTQIDRLFSKEELMTLIEKAILLFREQGNTGERFGI
                     TIDRIGADKFIEMLLSDEVLERKEEILEALLHLTGGAVC"
     gene            complement(6468..6731)
                     /locus_tag="KRL67_003253"
     CDS             complement(6468..6731)
                     /locus_tag="KRL67_003253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1883 domain-containing protein"
                     /protein_id="HBH1524098.1"
                     /translation="MEYSYSKINLKKGDIVEVNLEKQANVILLDHINYVKFKNQKNYD
                     YYGGFAKKNPCRMRVPNTGIWYLVVNQDGNSGIVNFSINTIQN"
     gene            complement(6830..7075)
                     /locus_tag="KRL67_003254"
     CDS             complement(6830..7075)
                     /locus_tag="KRL67_003254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH1524099.1"
                     /translation="MFIDEELEGYILTCKISEDFKNIPEYSDEEFYVTVYKDESSDSG
                     YYALLENKEERVVWDGEVVANNIFNNLWIVVNKVKTG"
BASE COUNT         2566 a          945 c         1155 g         2436 t
ORIGIN      
        1 atatataagg agtgatgtta ttgaaaaaag atagaataac tttttcggaa attttaatat
       61 taattgtagg ctgtatatta atggcaattt ctcttaactt attttttaat ccacatgcaa
      121 tcgctgctgg tggtataaca ggacttggag ttgttttaaa ttcattattc ggtgtagaac
      181 tttggattgt caatttactt ttaaatgttc cattattcat atttgcttac aaaatacttt
      241 caaaaaaaga ttgttttaaa acggttttag gaataatttt tcttaccata gcacttaagc
      301 ttactgcaaa tatggctaca ttagatataa caaatgatat gtatcttgca attatatctg
      361 gttctatttt aatgggagtt ggtcaaggac ttatttttag aataaatggc tctacaggtg
      421 gaactgattt aatggcactc ctactaaata aatattttcc tacatttagt atacctgttt
      481 taatgggtat tgtagactgc gttgtagttg ttttatcagg tatagtgaat agacaggttg
      541 aaattgcact ttattcaact gtggctttat acatcttagt aaaagttagt gacttgctta
      601 ttgaaggatt taattattcc aaatcattca ctattatatc cgacctttca aaagatataa
      661 gtaaaaaaat aatggaagat ttagatagag gtgcaactat tctaaaagga gaaggtgcat
      721 atactggaga aaataaaaat gtacttttag tagtagttga aaagaaagaa gttgtagaat
      781 taaaaaagtt agtaaaaaat gtcgacccta atgcgtttat tataattaca gatatacatg
      841 aagctttagg taatggattt aaaaaaatag aataactaac aaataaatag caagtataat
      901 taacatatac aaattcaact ttttatatgt tatattatac ttgctattta tatattataa
      961 ttgttgtttt taagataaaa attaaatgta tcaattattg ttttatacaa atatttccaa
     1021 acttgctctc atttaaatag tatgcttaga aattttagaa ttattgactc taactttatt
     1081 acttacttca ctttataaca atcaaatacg agtttaaata ttataaaact acaaaataat
     1141 ttaaagcata taatgtcaat atgtttaaat tataaaatat tcatgtagtt atataataaa
     1201 taatttataa atatagccaa cttagcctaa atacgtatat aatatagcta atatggttct
     1261 tatacttgat accatcaata aaactaaaaa tataaaaagg agaacttaaa atgagtgtta
     1321 gtttacaaaa aggacaaaga gttagcttag taaaagataa taatcctgta aaaaatctag
     1381 tagtaggctt aggatgggat atgaataaat tgggtaaaaa aaactatgac ctagatgcat
     1441 ttgcaatagc attgactaac caagataaaa tgaatgctag aggtgacttg gtttattttg
     1501 gaaatcttaa acacccttct aaagctatta tacacactgg agataactta actggaaaag
     1561 gcgaaggtga tgatgaacaa ataattgtta atttagaaga catacctgaa tatgtacata
     1621 aaatagtttt tggagtttct atatacaaag ctaaaaaaag agaccaggat tttggtcaaa
     1681 tgaacaactc ttttataaga ttaattgatt caaattccaa gcaagaatta tttaaatata
     1741 atcttcaaga agacttttct ggtaaagtaa cagttttggc aggagaaatt tatcgtagaa
     1801 atgaagaatg gaaatttaat gctttaggaa ttggtcaaaa tgaagagctt cgtgaattaa
     1861 ttaatacata taagtaataa ttacaataaa aaatttataa tgaggagttg gtttaaatgg
     1921 gtataacttt agcaaaagga caaaaagtaa gtttaacaaa aagtaaccct ggtctaaaaa
     1981 aagtaatagt aggactagga tgggatatta ataaatatga tggtggcttt gatttcgatt
     2041 tagatgcttc tgcattttta actggaactg atggaaaagt aacgaatgat ggagacttta
     2101 tattctataa taatttaaaa cacgcttctg gagctgttga atatatgggt gataatagaa
     2161 caggtgttgg tgatggtgat gatgaacaaa taaatgtaga cttatctaaa atacctcaaa
     2221 atattgctaa aatatcattt agtgttacta taaatgaagc aattacaaga agacaaaatt
     2281 tcggacaagt tgaaaattca tatataagaa tatataatga agaaactaat gaggaactta
     2341 ttaaatatga attgggtgaa gattttagca tagaaacagc aatagttgtg gcagaactat
     2401 atagacataa tggagagtgg aaatttaatg ctttaggttc tggatttgag gatgggcttg
     2461 caggattgtg taaaaatttt ggcgttaata taggctaata tatctaaata aaaataagag
     2521 tatattgata tactcttatt tttatttaga tatattataa actgtttaca tttctactct
     2581 tctttctcgc ttgttttttc ccatattcta ctgaaatatc aaaaaatgct gaaatgtttt
     2641 ctgtaaaaaa cttgcttcta tgatatgcaa tatcaaatgt cctatgtaag ttaatacctt
     2701 caatatcaca agcccacaaa agatgctttt ttaaataatc ctcaataagt aattcagata
     2761 taactgcaat gccaaaatta tctacaactg ctcttaatat agcttcagag ttataacatg
     2821 accatctaat attcattggg atttttaatt cttttaattg cttttctaat tgtgctcttg
     2881 taccacttcc taattcacgc ataattaatg gttgatttga aagttctgaa acttttatag
     2941 agtctctttt gtagaactca tgcttgtgtg aacatataag tacaagatta tcattaataa
     3001 tgctttttgt aacaatatct gggtgcttaa tttttccttc tacaatcgca atatctaatt
     3061 cactattaag taattttttt gcaatcagtc ttgtatcttc tacaaatact tcaggttcta
     3121 taaaaggatt taccttgtat agttctaaca ttattgggct tattacacat gtaccaacag
     3181 ttactgttgc cccaactctt atacggatat tttttgcaca atgcttcata caatcattca
     3241 tttcttcatt tacagctaaa agatgcctcc catatccaag aagttttttc cctgcctcag
     3301 ttaaatataa tttttttgaa attctatcaa atagcttaac accatactct ttttctattt
     3361 ctgaaatagc ttggctaact gatgactggg taatatacat atttcttgct acttcactca
     3421 ttttaccaca ttcagcaacc ttaacaaaga tttcaagact acgaattgtc atattatcac
     3481 aaccttagca aacattagta aatgcttatg attttattaa tattttagta ttttacaaat
     3541 acattgtcaa gaagtataat tttattatag agttataaag tagattggag tgtgatatat
     3601 tgtgaaagta ttaattatag gtggagttgc tgctggaaca aaaactgctg caaaaataaa
     3661 acgtgaaatg ggtgataatt gctctgttac aatactaaat aaaggcgatg atatttctta
     3721 cgcaggttgt ggtcttccat attacgttgg taaaataatt gaagacaaat catctttaat
     3781 cgtaaataca cctgaaagtt tttctaaact aacaggagca gaagttcgtt gtggagttga
     3841 agttacgtct attgatagaa atgcaaaaca agtaactttg aaaacgattt caacaggaga
     3901 aaaagaaaat ctaagctatg acaaacttgt aattgctaca ggggcagacc caataaaacc
     3961 accaatacct ggaatagatt tacctggagt attctttatg cgtactccaa atgatgctat
     4021 aaaactaaga gattctattg aaaacggtat taagagagct gttgttattg gtggaggttt
     4081 cattggtctt gaaattgctg aaaatctttc tgcaatgggt atacgtgtaa gtgttgttga
     4141 tatggctgaa catgttatgc ctggatttga tactgatttt gctgaatacg ttgaaaacca
     4201 tatggctgat aaaggcataa tgatttttac aggcgaccag gttgtgggta ttgaaggtga
     4261 aaataaggtt gaaaaacttc gtactaaaaa tagagctatt aaaacagatt tagtggttat
     4321 gtcagttgga atccgtccta acacaggttt tctaaaagac acagggttag aatttgctcc
     4381 taataataca gttattgcag atgaatatat gcttacaaat gacaaagata tctacgtagt
     4441 tggagactgt gcttttgtaa aaaattcttt aacaaataaa cctacatggt cacctatggg
     4501 gtcatctgca aatcatgagg gtagaatttg tgcacaaaat atatctggaa aatctaaaac
     4561 ttacaatggt gttcttggaa caacagtagt aaaattacct gagctcaatg caggaaaaac
     4621 aggtctaagt aaagaaatgg ctgaaaaaga aggttataat gtttgctctg taaccatagc
     4681 tactgatgat aaagctcatt attatccagg tgcatctaat tttattataa gaatggttgc
     4741 tgaaaaaaat actagaaaat tattaggtgt tcaagttatg ggacctggag cggttgataa
     4801 aatagtagat atcgctgcta ctgcaataac tttaggagct gacctttatt cattagaaag
     4861 tatggacctt gcatatgcac cacctttttc aacagctatt cacccttttg tagtggcagt
     4921 aaatgtttta caaaataaac ttaatggaga acttgatagt gttttacttg acgaaattga
     4981 agattttgac agttggacta aacttgatgt ttcaaaagct ccttctattc ctagtttacg
     5041 ttatattcct gttggtgaaa ttaatggaga aattgaaggt cttgctaagg atgaaaaaat
     5101 tcttcttata tgtgctaaag gtaaaaatag ctatatggcg caaaatcgct taagacgctt
     5161 tggctataca aatacgaaag ttcttgaagg tggaatacta ttttatccaa atcttaaggt
     5221 aatggaggag taaatatgag taatgttaca gcagaacaaa aaaaggaagt aaaaggtaga
     5281 ggttttctac caagcaggga tggagaacat tttgcagcac gtataataac tgtaaatggt
     5341 gtaataaatg catcacaagc aaaaaaaatt gcagaagctg ctgaaaaatt cggaaacggt
     5401 cagttagcat ttactactag actaacagta gaattacctg gtgtaaaatt tgaagatatt
     5461 gaagctctaa gtgaatttat cgctagcgaa aatctaataa caggtggaac tggatcaagg
     5521 gttcgccctg ttgtggcttg taaaggtact gtttgtgttc atggtctagc tgatacacag
     5581 gcacttgccg ctgaaataca cgaagaattt tacaaaaatt ggtatgatgt aaaactacct
     5641 cataagttta aaattggcgt tggtggatgc cctaataact gcattaaacc tgacctaaac
     5701 gatcttggaa ttgttggtca acgtgtacct gactatgatt cagaactttg tgttggttgt
     5761 aaaaaatgtg ctgttgtaga ggtctgtcct gttaaagcag ctaaattaac agataaaggt
     5821 aaacttgaaa tagatagtaa tctatgtaat aactgtggta agtgtataga aagttgtaat
     5881 tttgatagta ttgaagaaaa agaatctggt tataaagtct atataggagg aaaatgggga
     5941 aaatcagttc gtcctggaac acagattgat agattatttt caaaagagga acttatgact
     6001 ttaattgaaa aagcaattct tttatttaga gaacagggta acacaggtga acgttttgga
     6061 ataactatcg atagaatcgg tgctgataaa tttatagaaa tgcttttatc agatgaagtt
     6121 ttggaaagaa aagaagagat acttgaggct cttttacacc ttacaggtgg agctgtatgc
     6181 taacaataaa tattttaatt gtacaaataa catatgaaaa aaattttagt taagaataat
     6241 taaaattcta tgtatgatat atgataaatg gggctatctc aaaacacaga tacgcctctt
     6301 tttcattgtt gaaatgataa agtatatttg agttcataca aaaaagagtg tcccatgaat
     6361 tataaaattc attttgaggc acccacatta ttttataata attgcttatt aattttattt
     6421 taactatata aaatgtacaa aaagtaagaa tatccaatat tctaaaatta attttgaatt
     6481 gtattgattg aaaagtttac tattccacta tttccatctt gatttactac tagataccat
     6541 attccagtat taggaactct cattctacat ggatttttct ttgcaaatcc accatagtaa
     6601 tcataatttt tttgattttt aaatttcaca taatttatat ggtctaacaa tattacattg
     6661 gcttgtttct ctaaatttac ctctacaata tccccttttt ttaagtttat cttactataa
     6721 ctatattcca ttgaattact cctccagtat cgaataatct ttgttattta ctcatctcaa
     6781 atatataaaa attcatttaa aatattacaa atactatata attagactat tatccagttt
     6841 taaccttatt tactacaatc caaaggttat taaaaatatt attggcaaca acttctccat
     6901 cccatacaac tctttcttct ttattttcta ataaagcata gtacccagag tcagaacttt
     6961 catctttata gactgtaaca taaaactctt catcactata ttcaggtata tttttaaagt
     7021 cttcagatat tttacatgtt aatatatacc cttctaattc ttcatctata aacaaagcaa
     7081 ttcctccaat ctctatattt tt
//
LOCUS       DAEPXF010000184         7075 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain HB27
            SAMN13376389-rid12479423.denovo.191, whole genome shotgun sequence.
ACCESSION   DAEPXF010000184 DAEPXF010000000
VERSION     DAEPXF010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13376389
            Sequence Read Archive: SRR10518432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7075)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7075)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001069624.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001069624.1
            Genome Coverage       :: 315x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/15/2021 01:54:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,165
            CDSs (total)                      :: 4,076
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 89
            rRNAs                             :: 10, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 10 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 19 of 69
            Pseudo Genes (incomplete)         :: 44 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 10 of 69
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7075
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13376389-rid12479423.denovo.191"
                     /strain="HB27"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="China: Hebei"
                     /collection_date="2017"
     gene            129..1517
                     /locus_tag="KRL67_003255"
     CDS             129..1517
                     /locus_tag="KRL67_003255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH1524100.1"
                     /translation="MEDKFYAKGKGNNGYIKNLEVCSFNNLDGTCGMFQMALYKRDEK
                     YYLYGCCFGGNKKNGVMISDITDPYNPQFIKHFQMLDPKEYPTTTTPKIQIADDLMIV
                     AMSCGSGPGALVDQAKLANIKCEAGIRIYSLKEDPLNPKFLGYWDCGLKHVMGVHRFM
                     YNGGRYVHLSSDCVGFEGLIYRVIDIINPTNPVEIGKWWRPDQYADGYPNRIFDAGAP
                     HCPEFMDKGWLHGPPFVRDGKAYCGYGGAGLVVLDVEDLTRPRCLGELPFMPAFSSRL
                     AGARTHTALPLPGRDLVVVQNEGERFQFFKPDNITDVQAMNNIHMVDVSDPTKPTLIA
                     QFPYPEVPKDFPYPNFNVAGLGKPGPFGPHNLHEPMDNKPWLEQRGDRVYCCYFHAGL
                     RVYDVSDPYYIKELAYFIPPNPNKTPEESYFPGFPGPRLAVTEDLIVDDRGYIIIDAL
                     DDGFYILKIKED"
     gene            1545..2498
                     /locus_tag="KRL67_003256"
     CDS             1545..2498
                     /locus_tag="KRL67_003256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HBH1524101.1"
                     /translation="MKILGISFGTKNGNNDTMCKIALKGAKEAGAEVEFIQMSSINIK
                     HCTGCCACVKTLLSGKGSICVLKDDFEWLLDKMKDADGIVVSDPIFEEGASGLFHTIM
                     DRFGPRADRGNNIIGTKVAEAIGGKIPDSRMLNDKVISFMGIGGSDWGTRVQCEHAML
                     ALIPMWKVIDNAWFPWAKELVMDDTRLVQVHQIGLNIVEAAKDFEKASYQGEEGVCPH
                     CHNRLFYLEPGTKKAICSLCGIVGELDTISGKTIFSFPEEQLGHAHDTLPGKFIHGDD
                     IKKMEGHYAEVRKTQEFKERKTSYNFIEPLTKEKQAGISLG"
     gene            2558..2782
                     /locus_tag="KRL67_003257"
     CDS             2558..2782
                     /locus_tag="KRL67_003257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH1524102.1"
                     /translation="MNEKPSFKQIEVILAVIFLVLTYYKVRLTPAFIVIFIPLYLSYF
                     AVSMIVNQIAIKLLEIMQVREATNYNIGNG"
     gene            2775..3287
                     /locus_tag="KRL67_003258"
     CDS             2775..3287
                     /locus_tag="KRL67_003258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1097 domain-containing protein"
                     /protein_id="HBH1524103.1"
                     /translation="MDRKKKEILTLSMGIALLPPLWAVLALYMGIKTGAVALICAGLY
                     VTNGNKQKDGLKIMFGFWCGDLWAVLAILIMGYMNFNQNLELFLTLSILGFFAVVIAS
                     LFEKIIFLPSWLCGWAIGLTIMTGENIINLQDICIQIGVAMSVGVWYVGAGVDLFTRY
                     ILSKDKKKRN"
     gene            3320..4174
                     /locus_tag="KRL67_003259"
     CDS             3320..4174
                     /locus_tag="KRL67_003259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011862019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA hydrolase"
                     /protein_id="HBH1524104.1"
                     /translation="MVMGFEFKIMRSLIYVGLAKEEYRPKLMDWLYRHHIPDSISTFG
                     PYCTKYAFYQAYPTPNEGERFGVRKMQLTEHYWLVDEHMPEMANRIMTEYMPMDVLRW
                     QGCIPDVENKRVHENAESGDAGRAVGGDNGCPPFIFAFVPINWEEDFRGKGRTVQDGP
                     NYRWQFMIKYPDGISKEEGEKWFYDEVVPYFTNCCYVNRFVSSKIMINYGATAFDRVS
                     ELWFEGEEEWYKAVVEETKSFIKKPEWAQEEEFPYLKPQFNIASVFLGDIATMDAYSQ
                     YRGYIPMR"
     gene            4708..5502
                     /locus_tag="KRL67_003260"
     CDS             4708..5502
                     /locus_tag="KRL67_003260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HBH1524105.1"
                     /translation="MYTIGQVSKFLGISRDTLKFYEDKGLVNPKKNDENGYRIYNNFD
                     IYDIATINFYREIDIEIKKIQEIRKSKSINNLELLLEEKEQIILKEIEYKKLLLKKVQ
                     LVKEDCDKIKTYFGKYTIKEMKPLEVKGRIKHVTDYDEYGALKENTNNLKKAVTLTDL
                     RRVISFNEEGIIEEKFIIVKEVDDYNKIIEGEVLSHPNCIYTIIEDGRWSTGGENIDN
                     KVEDRLREIAIKNEYELLGIVYVNILLTTYEEEIERIFLEIYAPIK"
     gene            5606..6412
                     /locus_tag="KRL67_003261"
     CDS             5606..6412
                     /locus_tag="KRL67_003261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HBH1524106.1"
                     /translation="MGTEKSLKRPVASFIILTNIIFLPLFLLVVVTIILKLPTVISDI
                     ALCISAWSSTFAFMILFKKIYPGKKFLVHVKDRFRNKLKFSTVSIIISIQVLIAVVVI
                     FILASKNHGIMPNFTVSSWGAFIYLFLKNLFAGSLGEELGWRGFVQNHLQKRYSPLKA
                     SIIVGFWWGMWHLPIWFTTGFKGLDLIKYIIFFMIAIISISIIMTTFYNLNKTLIIPI
                     AIHQLFNFLIGIINGDLIELINYYAMLYLIFAIALIVINPGNVLYKNIRE"
     gene            6474..6995
                     /locus_tag="KRL67_003262"
     CDS             6474..6995
                     /locus_tag="KRL67_003262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003374619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HBH1524107.1"
                     /translation="MVLLIVDAQKLITNERLYKFNEFVVNVENLIDTARKNNIEVIYV
                     RHDDGVENELTKGKNGFEIYEKFKPCNGEKIFDKKVNSAFKETGLLEYLTNNGEKDII
                     IAGLQTDYCIDATIKCGFEHGFNIIVPAYSNTTVDNFFMSAEQSYKYYNEFIWNERYA
                     KCISLEETLRKMK"
BASE COUNT         2720 a          778 c         1328 g         2249 t
ORIGIN      
        1 gattttataa atttattaat aagaataact tatatattcc catgaaaacg atattggatt
       61 taagtaagtt tgtctcataa aatgaaagta tatcaattga taatacttca aataagaagg
      121 gagaaaaaat ggaagataaa ttttatgcaa aaggcaaagg aaataatgga tatattaaaa
      181 atcttgaagt ttgttccttt aataacttag acggaacttg tggaatgttt caaatggctc
      241 tgtacaaaag agatgaaaaa tactatttat atggatgctg ttttggagga aataaaaaaa
      301 atggagtaat gattagtgat attacagacc cttataatcc acaatttata aaacattttc
      361 aaatgttaga ccctaaagag tatcctacaa caacaactcc aaaaattcaa atagcagatg
      421 atttaatgat agtagcaatg agttgtggaa gtggaccagg agcacttgtt gaccaagcta
      481 aattagcaaa tattaagtgt gaagcaggaa ttagaatata tagtttaaaa gaagaccctt
      541 taaatcctaa gtttttagga tattgggatt gtggcttaaa gcatgtaatg ggtgttcata
      601 gattcatgta caatggtgga agatatgtac atttatcaag tgattgtgtt ggctttgaag
      661 gtctgattta cagagtcata gatataataa atcctactaa tccagtggaa ataggtaaat
      721 ggtggagacc agaccaatat gcagatggat acccaaatag aatttttgat gcaggagcac
      781 ctcattgccc agaatttatg gataaaggat ggcttcatgg acctccattt gtaagagacg
      841 gaaaagcata ttgtggttat ggaggagctg gtttagttgt gttagatgtt gaagatttaa
      901 caagaccaag atgcttaggt gaattgccat ttatgcctgc attttctagt agacttgcag
      961 gtgcaagaac tcatacagca ttaccattgc caggaagaga tttagtcgtt gttcaaaatg
     1021 aaggagaaag attccagttc tttaaaccag ataacattac agatgttcaa gctatgaata
     1081 atatacatat ggttgatgtt agtgacccaa caaaaccaac attaattgct caattcccat
     1141 atcctgaagt gccaaaagat ttcccttatc ctaactttaa tgttgcagga ttaggaaaac
     1201 cagggccatt tggtccacat aatcttcatg aaccaatgga taataagcca tggttagagc
     1261 aaagaggaga tagagtatat tgctgttatt tccatgcagg gttaagggtt tatgatgtat
     1321 cagacccata ttatatcaaa gagctagcat attttatacc accaaatcca aataaaacac
     1381 cagaagaatc ttatttccca ggattcccag gaccacgctt ggcagtaaca gaagatctta
     1441 ttgttgatga tagaggctac atcatcatag atgctttaga tgatggattc tatatattaa
     1501 aaataaaaga agattaaata aatactaaag agggagagtt aaaaatgaaa atactaggta
     1561 tttcttttgg aacaaaaaat ggaaataatg acacaatgtg taaaattgca cttaaaggtg
     1621 ccaaagaagc aggggctgaa gtagaattta ttcaaatgtc atctattaac attaaacatt
     1681 gtactggatg ttgtgcgtgc gttaagactt tattatctgg aaaaggtagt atatgtgtgt
     1741 taaaagatga ttttgaatgg ttgcttgaca aaatgaaaga tgcagatggt atagtagttt
     1801 cagaccctat ttttgaagaa ggggcatcag gattattcca tactataatg gaccgttttg
     1861 gacccagagc agatagagga aataacatta taggaacaaa agttgcagaa gctataggag
     1921 gaaaaattcc agattcaaga atgcttaatg ataaagtaat atcatttatg ggaataggag
     1981 gttctgactg gggtacaaga gttcaatgtg aacatgctat gctagctctc ataccaatgt
     2041 ggaaagttat agataatgct tggttcccat gggcaaagga attagtaatg gatgatacaa
     2101 ggcttgttca agttcatcaa attggtttaa atatagttga agcagcaaaa gattttgaaa
     2161 aagcttcata tcaaggagaa gaaggtgtat gtccacattg ccacaataga ttattctacc
     2221 ttgagccagg tactaaaaag gcaatttgtt ctctatgtgg tatagttgga gagttagata
     2281 ctataagtgg aaaaactata tttagttttc cagaagagca attaggtcat gctcatgata
     2341 ctttaccagg taagtttata catggtgatg atattaagaa aatggaaggt cattatgcag
     2401 aagttcgtaa aacacaagaa tttaaagaaa gaaaaacatc ttataatttt atagagccat
     2461 taactaaaga aaagcaagct ggaatttcat taggataata attaaaatac taagactctt
     2521 ttttaaggga tgttagatgt tataggaggc ttattgtatg aatgaaaagc caagttttaa
     2581 acaaattgaa gttattttag cagttatttt tttggtactt acatattaca aggttagatt
     2641 aactccagca tttattgtta tatttatacc actatattta agttattttg cagtatctat
     2701 gattgtaaat caaatagcaa ttaagctatt ggagataatg caagttaggg aagctactaa
     2761 ttacaatata ggaaatggat agaaagaaaa aagaaatatt aacactgtct atggggattg
     2821 ctttactacc tcctttatgg gcagtattag cactttatat gggaataaaa actggagctg
     2881 tagcactaat ctgtgcagga ttatatgtaa ctaatggtaa taaacaaaaa gatggattaa
     2941 aaataatgtt tggtttttgg tgtggagatt tatgggcagt gttagccatt ttaattatgg
     3001 gatatatgaa tttcaatcaa aacctagaat tatttttaac acttagtata ttaggttttt
     3061 ttgcagttgt aattgcatcc ttatttgaaa aaataatttt cctaccatca tggctttgtg
     3121 gttgggctat aggtctaact attatgacag gtgaaaatat tattaattta caagatattt
     3181 gtattcaaat tggtgtagct atgtcagtag gagtatggta tgtgggtgca ggtgtagatt
     3241 tgtttacgag atacattttg agtaaagata agaaaaagag aaattgaaag catgtgaata
     3301 tactttgatt ataggagata tggttatggg ttttgaattt aaaataatga gaagtttaat
     3361 atatgtagga cttgctaaag aagagtatag acctaagtta atggactggt tatatcgtca
     3421 tcatattcca gatagtatta gcacttttgg accatattgt actaaatatg ccttttatca
     3481 agcatatcct acaccaaatg aaggtgagcg ttttggtgta cgtaagatgc aactaacaga
     3541 acattattgg cttgtagatg aacatatgcc tgagatggca aatagaatta tgacagaata
     3601 catgcccatg gatgttctac gttggcaagg gtgtatacca gatgtagaaa ataaaagggt
     3661 tcatgaaaat gcagaaagtg gagatgcagg acgtgcagta ggtggagata atggatgtcc
     3721 accatttata tttgcctttg ttccaataaa ttgggaagaa gattttagag gaaaaggacg
     3781 tactgtacaa gatggaccaa actatcgttg gcaatttatg attaagtatc cagatggtat
     3841 ttctaaagaa gaaggagaaa aatggttcta tgatgaggta gtgccatact ttacaaactg
     3901 ttgctatgtt aatcgttttg tcagtagtaa aataatgatt aattatggag caactgcttt
     3961 tgaccgtgta tcagaactat ggtttgaagg ggaagaagaa tggtataaag ctgtggttga
     4021 agagacaaag tcgtttatta aaaaaccaga atgggcacaa gaagaggagt tcccatattt
     4081 aaaaccacaa ttcaatatcg catcagtatt cttaggtgat atagcaacta tggatgcata
     4141 ctcacagtat cgtggatata taccaatgag ataaaaatta tagtttgtga taagtagagg
     4201 aggcatttac tcttactggt agaagaagac ttattgtatc cttaaaagta ggtgaatata
     4261 tagttttata tttaaattta tataaaacta agactaataa atgtgaaaca atagaattag
     4321 aataatttga taatatttgt taaaagagta tttagaatgt gtttaaagat taagatgttc
     4381 cttttatggt ggcagatagt tgaaataata aaactgttat catgaaagga atatttttat
     4441 gtatatgaaa gcttttaaaa taaaagttaa atatgtttaa gaacatctta aagtgaaaga
     4501 accattatta gaaatatgta gacaatatag tatttataac gtatcatatt aacaagttta
     4561 cacttgggtt atgaaacaaa aagagtaatt caaaagattg taaatactat gctgactttg
     4621 atacttcaaa gtttagatga tactgtaatt gttaatagaa atacgtttaa tgagcataaa
     4681 gaatttattt aataatagga gaatataatg tatacaattg gacaagtatc taaattttta
     4741 ggtatatcta gagacacact aaaattttat gaggacaaag gattagtaaa tcctaagaaa
     4801 aatgatgaaa atggatatag aatatataac aattttgata tttatgatat tgcaacaata
     4861 aacttttata gagaaattga tatagaaatt aaaaagattc aagaaataag aaaaagtaag
     4921 agtataaaca atttagaact cttactagaa gagaaggaac aaatcatact aaaagagata
     4981 gaatataaaa agcttctctt aaaaaaggtt cagttagtca aagaagattg tgacaaaatc
     5041 aaaacatatt ttggaaaata cacaataaaa gaaatgaagc ctttggaagt aaagggtaga
     5101 ataaaacatg ttactgacta tgatgaatat ggggccttaa aagagaatac aaacaactta
     5161 aaaaaagcag ttacattaac tgatttaaga agagtaataa gttttaatga agaaggtata
     5221 atagaagaaa aatttataat tgtaaaggaa gtagatgact ataacaaaat catagaaggt
     5281 gaagttctgt ctcatcccaa ttgtatttat actattattg aagatggcag atggtcaact
     5341 ggaggagaaa atattgataa taaagtggaa gacagattaa gagaaatagc aataaaaaat
     5401 gaatatgaat tacttggaat agtctatgtt aatatattgc ttacgactta tgaagaagag
     5461 attgagcgta tatttttaga gatttatgca cctataaaat aatataaaag cattgacctg
     5521 ggacttactc ataggtttaa actcatgata taagcagtta taacttatat agcagttaaa
     5581 tattaatttg aaatgaggat tacttatggg aacagagaaa tcattaaaaa ggcctgtagc
     5641 cagttttatt attctaacaa atataatttt tttaccactt tttttattgg tagtagtgac
     5701 tataatttta aaacttccaa cagtgatatc tgacattgcg ttgtgtatat cagcgtggtc
     5761 atccactttt gcatttatga tattatttaa aaaaatttat ccaggtaaga aatttttagt
     5821 acacgtaaaa gatagattta gaaataaact taaattttca acagtatcta taataattag
     5881 tatacaagtc ctaatagctg tagtagttat atttatatta gcatctaaaa atcatggaat
     5941 aatgcctaac tttacagttt catcatgggg ggcatttata tatttatttt taaaaaattt
     6001 atttgcagga tcattaggtg aggaattagg ttggagagga tttgttcaaa atcatcttca
     6061 gaaaagatat tctcctttaa aagcatccat aatagtaggt ttttggtggg ggatgtggca
     6121 tttaccaata tggtttacaa caggattcaa agggcttgat ttaataaaat atattatatt
     6181 ttttatgatt gcaattatat caatcagtat tataatgaca actttttata atctaaataa
     6241 aactttgata atacctattg caatacatca actatttaat tttttaatag ggataattaa
     6301 tggagattta atagagctta taaattacta tgcaatgtta tatttaatat ttgcaattgc
     6361 attaatagtt ataaatccag gaaatgtatt atataaaaat attagagagt aatatatatc
     6421 tttatggtaa gatataaaga aattattaga agaacatcca aggaggaata aacatggttt
     6481 tactgattgt tgatgcacaa aagttaataa caaacgagag gttatataaa tttaatgaat
     6541 ttgtagtaaa tgtagaaaat ttaattgata cagctagaaa aaataatata gaagtaatat
     6601 atgtacgtca tgatgatgga gttgaaaatg aattgactaa aggtaaaaat ggctttgaaa
     6661 tatacgaaaa atttaaacca tgtaatggag aaaaaatatt tgataaaaaa gtaaatagtg
     6721 cttttaaaga aactggtcta ttagaatacc taacaaataa tggtgaaaaa gatattatta
     6781 ttgctggtct ccaaacagat tattgtattg atgccactat aaaatgtgga tttgaacatg
     6841 gatttaatat aatagttcca gcttattcaa atacaactgt tgacaatttt tttatgtcag
     6901 cagaacaatc atataaatac tacaatgaat ttatatggaa tgaaagatat gcgaagtgta
     6961 tttcattaga ggaaacactt agaaaaatga aataaaaata gagtatgaat tgtatatgta
     7021 cataagttga tttcataaaa aaggatgtta aattccaagt taaaaattag attaa
//
LOCUS       DAEPXF010000185         7011 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain HB27
            SAMN13376389-rid12479423.denovo.192, whole genome shotgun sequence.
ACCESSION   DAEPXF010000185 DAEPXF010000000
VERSION     DAEPXF010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13376389
            Sequence Read Archive: SRR10518432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7011)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7011)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001069624.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001069624.1
            Genome Coverage       :: 315x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/15/2021 01:54:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,165
            CDSs (total)                      :: 4,076
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 89
            rRNAs                             :: 10, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 10 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 19 of 69
            Pseudo Genes (incomplete)         :: 44 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 10 of 69
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7011
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13376389-rid12479423.denovo.192"
                     /strain="HB27"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="China: Hebei"
                     /collection_date="2017"
     gene            242..1093
                     /locus_tag="KRL67_003263"
     CDS             242..1093
                     /locus_tag="KRL67_003263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3100 domain-containing protein"
                     /protein_id="HBH1524108.1"
                     /translation="MEINKKHFIQVFLTCLILVVICEFIGQRTISIGKASIVLFPMLY
                     AVIIGLIITPDLLGKKIKALKKVVGEKEINIAGEVVGIALVMLGIKYGTTVGPNIDKI
                     IQAGPAFIAQEFGHILAPIVALPLALMFGMKREAVGATASISREPSLGVISEKYGINS
                     PEGSGVLGTYLMGTVLGTIYFSVLGSISIYSGLHPYALGMACGVGSGSMMTAAAGSLS
                     TMVPPEMADTILAYAATSNMMSSITGIIFLVFVSLPFTNFMYKILEPKFSRNKKTKEI
                     KSTEGEC"
     gene            1097..1567
                     /locus_tag="KRL67_003264"
     CDS             1097..1567
                     /locus_tag="KRL67_003264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH1524109.1"
                     /translation="MFANLNLKKSSYQLLVMSVIGVIILIGQRISVGTSIVTALPGMV
                     MLILAAMAAMIIKDLFPKSIFPAFGFATIIGLLLSMPYSPTSEVFLTNTNNINFMAIT
                     TPLLAFAGISVGNKIEALKEMSWKIVIISLIVFTTIFFACASIAHIVLSIQGKI"
     gene            1580..2890
                     /locus_tag="KRL67_003265"
     CDS             1580..2890
                     /locus_tag="KRL67_003265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HBH1524110.1"
                     /translation="MNKEQLKSLVIKTIDENREKILKIGQGIYKNPEYGYKEFKTTEA
                     VSNFFEQELNLSVEKNIAVTGCKADANSSKKGPHISILGELDGISCKEHKDANEIGAS
                     HTCGHNIQIAGMLGAAVGLVKSGVLEHLDGKVSFMATPAEEFIELEYREQLKKEGKIT
                     YFGGKQELIKRGYFDDIDISMMFHASDLGEDKALVGPESNGFVGKKVKFIGKESHAGS
                     APHDGINALNAAMLAINNVNALRETFKESERVRFHPIITKGGDIVNVVPADVHMESYV
                     RARTINGMIDANERINKALMAGGMAVGAEVEITEIPGYLPILRYKSLDNLFKNNLEDL
                     GLKGKVIDGGDFTGSFDFGDVSHIMPTLHPMIGGVKGALHTRDFEIVDEDLAYIIPAK
                     AMALTVVDLLFNNAKDAKDILDAFTPVMTKEEYLTFLEKHDKTL"
     gene            3102..3848
                     /locus_tag="KRL67_003266"
                     /pseudo
     CDS             3102..3848
                     /locus_tag="KRL67_003266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901983.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
     gene            4208..5749
                     /locus_tag="KRL67_003267"
     CDS             4208..5749
                     /locus_tag="KRL67_003267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbgT family transporter"
                     /protein_id="HBH1524111.1"
                     /translation="MNDNVVKKKNFFDRTLNRIETVGNKLPDPVTIFLGLCVLLLILS
                     SLVGSMGISVVHPGTGETITAVNLLTVEQLQILLGNIVSNFQGFAPLGLVLVTMIGAG
                     VCDKTGLMTATIKASVSKIPETRVTLVVMTIGMLANIASDAGTILFPPLAALVYLGVG
                     RHPLIGLFSGYAAVCLGFAANIMISVNDILAASFTVPAAQMIDSGFQANATMNLFFMI
                     ASTFVLILLATWVTEKIVAPRFGKYEGNAELDVDGSLSNVEKKGLKKAGISILIYVAI
                     IIALSVIGKKPFLADPETGDLLSNNAPLMKGMVPIIALAFFIPGVVYGKTIGKIKKDK
                     DVVSIMASAMSDMGGYIILAFAASQFLQLFTNSNLGLILAVKGGEFLKSAGINGPLLL
                     IGFIILSCFINLFIGSASAKWAILAPIFVPMFMMVGYNPALTQMAYRIGDCITNPLSP
                     LFPYFPIILAFTRKYDKDAGMGTVIANMIPYSISFLIVWTILLILFMVFNIPLGPGIL
                     PSYSM"
     gene            5784..6890
                     /locus_tag="KRL67_003268"
     CDS             5784..6890
                     /locus_tag="KRL67_003268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HBH1524112.1"
                     /translation="MLDKNRLINNFMDMVRIDSPSNQELEMSKWLVNYLKERNIDAII
                     DDAGEKYGGNTGNVIAYIKGEEGSRPLCLCAHMDQVQPCLGVKPILDGNVVRSDGTTT
                     LGADDKAGIAAILEALEHVITEKIPHRDIYLCFTICEEAGMHGVKNFNPDNLPCKDMV
                     ILDSGGAIGSIAYKAPAQQSIKISFHGKKAHAGIEPEKGLNAILVASYAISNMHIGRI
                     DSLTTSNIGKIEGGGATNIVTDKVTLTAEIRSHIPETLEYELNHMEKCCKDAASKFNT
                     TYTFEHNMSYPSFELSRDSHVFKLSEEAIRQVGIVPNPMVIGGGSDANILANLGYNCA
                     ILSLGMYDVHTVNEYVNIDELYDTAKIVYHMIKL"
BASE COUNT         2485 a          923 c         1209 g         2394 t
ORIGIN      
        1 ttaaacaatt ttttcataat aaagctatat tacaatatat tcaaaatatg taatatttta
       61 gatatttttc gttgacaatg ttaaattttt gttttattct taattaagga catgttattg
      121 acatttctga aacttatttg aaatagtttt ttcattttat ttaggaaaac gtattatact
      181 taagaaaacg tattttctca tttcagataa gtaaaatatt tattcttttg gggaggaaat
      241 tatggaaatt aataaaaaac attttattca agtattttta acttgcttaa tcttggtagt
      301 aatatgtgaa tttatagggc aaagaactat atctattgga aaagcctcta tagtgctgtt
      361 ccctatgctt tatgcagtta taattggact tataataaca ccagatttat taggtaaaaa
      421 aattaaagca cttaaaaaag tagttggaga aaaagaaata aatattgctg gtgaagttgt
      481 tggaattgca ctagtaatgc ttggtataaa atatggtact acagttggac caaacattga
      541 caagataatt caagctggac cagcatttat cgctcaagaa tttggtcata tacttgcacc
      601 aatcgttgca ttacctcttg cattaatgtt tggaatgaaa agagaagctg ttggagctac
      661 tgcaagtata agtcgtgaac cttctttagg agttatatca gaaaaatatg ggataaactc
      721 accagaaggt agtggtgtac ttggtacata tttaatggga acagttcttg gtactatata
      781 ctttagtgta ctaggttcaa tatctattta tagtggtctt cacccatatg ctttgggaat
      841 ggcctgtggt gttggtagtg gaagtatgat gacagcagct gctggttcat tatctaccat
      901 ggtaccacct gaaatggcag atacaatact tgcctatgct gcaacaagca atatgatgtc
      961 tagtataaca ggaattatat tcttagtttt tgtatcctta ccatttacta actttatgta
     1021 taagatttta gaacctaaat tttcgagaaa taaaaagact aaagaaatta aatctacaga
     1081 aggagagtgc taataaatgt ttgctaattt aaatttaaag aaaagttcat atcagttatt
     1141 ggttatgtct gttattggag ttataattct tataggacaa agaattagcg ttggtacatc
     1201 tatagttaca gctcttcctg gtatggtgat gctaatatta gctgctatgg cagctatgat
     1261 tataaaagat ttatttccta aatcaatctt tccagctttt ggatttgcaa ctataatcgg
     1321 tcttttactt agtatgccat atagtccaac atctgaagta ttcttaacta acactaataa
     1381 tattaacttt atggctataa ctactccact actagctttt gcaggaattt ctgttggaaa
     1441 taaaatagaa gctttaaaag aaatgtcatg gaaaatagtt ataatttcac ttattgtatt
     1501 tacaactata ttctttgcat gtgcgagtat agctcatata gttttaagta ttcaaggtaa
     1561 aatataggag gaatttatta tgaataaaga acaattaaaa tctctagtaa taaaaactat
     1621 agatgaaaat agagaaaaaa tattaaaaat aggtcaagga atatacaaaa atccagaata
     1681 tggatataaa gaatttaaaa ctacagaagc tgtttcaaac ttttttgagc aagaattaaa
     1741 tttatctgta gaaaaaaata tagcagttac aggatgtaaa gcagatgcaa attctagcaa
     1801 gaaaggtcct catatatcta tacttggaga attggatggt atatcatgta aagaacataa
     1861 agatgctaat gaaataggtg cttctcatac ttgtggacat aatattcaaa tagctggaat
     1921 gttaggtgct gctgttggtt tagtaaaaag tggcgtttta gaacatttag atggtaaagt
     1981 atcttttatg gctactcctg ctgaagagtt tattgagttg gaatacagag aacaattaaa
     2041 aaaagaagga aaaataactt attttggtgg aaaacaagaa ttaataaaaa gaggttattt
     2101 tgatgatata gacatatcta tgatgtttca tgcttctgat ttaggagaag ataaggctct
     2161 agttggtcct gagagtaatg gatttgttgg aaaaaaggtt aaatttatag gtaaagaatc
     2221 tcatgctggt tctgctccac atgatggaat aaatgcttta aatgcagcaa tgcttgctat
     2281 aaacaacgtg aatgcactta gagaaacttt taaagaatct gaaagagtta gattccaccc
     2341 tatcatcaca aaaggtggag atatagtaaa tgttgttcct gctgatgttc atatggaatc
     2401 atatgttaga gctagaacta taaatggtat gattgatgct aatgaaagaa taaataaggc
     2461 tttgatggct ggtggaatgg cagttggagc tgaagttgag ataactgaaa ttcctggata
     2521 tcttccaata ttacgataca aaagtcttga taatttattt aaaaacaatc ttgaagattt
     2581 aggtctaaaa ggaaaagtta ttgatggtgg agattttaca ggctcttttg attttggaga
     2641 tgtgtctcat ataatgccta ctcttcaccc tatgattggt ggtgtaaaag gagctcttca
     2701 tacaagagat tttgaaatag ttgatgaaga tttagcttac ataattcctg ctaaagcaat
     2761 ggcacttaca gttgtagacc ttctttttaa taatgctaag gatgctaaag atatattaga
     2821 tgcctttact ccagttatga ctaaagaaga atatctaaca ttcttagaaa aacacgataa
     2881 aactttataa gtctattttt ataatagggt ggtatctact gtaaaagtta taccactctt
     2941 tgtttttgta acagtatact tcttttcttt tagtttttaa attaaaccaa aatatattat
     3001 cctccttaaa acttttagag ctacgttgaa atatcagtag aactatgata tattctttct
     3061 aattaatata aatttctatt ctaattcaag gtggtgaaaa aatgtttaat gcaggaaatc
     3121 gaattataaa tacatggttg taccctattc aagatggctt tgtcttgatt gatactggat
     3181 atgaaaatgg ttttaaacat ttcaaaaaac gaatttctaa attcaacata aaaatcaaag
     3241 atattcgata catatttcta actcatgctc atgatgacca tgttggtttt ctaaatcaga
     3301 ttctagacga atctcctaat gcaaagattg ttatgcatga taaagcttta gaagttctat
     3361 ataaaggtca aaactctttt attggtggtt gtaccagtcg tactgccttt ctcttttgcc
     3421 aaattatgaa actatttgga aaaggtgagc atcgtttttc tccacttaaa caagaatttg
     3481 aaaaaaggtg tattcttgtg tctgacaaga atcgtaaaga aatttaaaaa gaactaaatg
     3541 gaaaaattat agatacacca ggacatactt ctgattcaat ttcactacta attgatgatg
     3601 gtgttttatt ttgtggagat tctgcaatga atggatttcc aagtactcac aaaattacca
     3661 tatttacaga aaacaaacta gaatttattc aatcttggaa aacaatcatc aatacaaaac
     3721 ctaaaatgat ttatcctgga catggaaaac catttgaata cagtaaatta gaacaaaatc
     3781 taatctatgt aaataaaata aatttatatc ctctacatga ttcaaaagat attcacagat
     3841 ttttgtagaa aatacaaagt aaatttcttt gattaattaa aaaacctaac actttactac
     3901 actacttatt ttaataactt aatcttccac tagattttta taaaattttg ttttaaaatt
     3961 aattttaaag gttatatact gcacagatat attaaataca attcgtcata aatctactta
     4021 aagcatattt aactagcact actcaaccag aaaattcaga aaattgcatt gacataggaa
     4081 aaagtttatt ttatactttt tttaggacat attgtggaca cgtcttaaat cgacaagtag
     4141 aatttacatt gcacgcttaa ccacaatact ttttttaatt atatttttaa acattaggga
     4201 ggatattatg aatgataacg tagttaagaa gaaaaacttt tttgacagga cattaaacag
     4261 aatagaaact gttggtaata aactaccaga cccagttaca atatttttag ggttatgtgt
     4321 acttttactt atactatcta gtctagttgg ttctatgggt atatcagtag ttcatcctgg
     4381 tactggagaa actataactg ctgtaaattt attgactgta gaacagcttc aaattctttt
     4441 aggaaacata gttagcaatt tccaagggtt tgctcctttg ggtctagttc tagtaactat
     4501 gataggtgct ggtgtatgtg acaaaacagg acttatgaca gcaacaatca aagcatcagt
     4561 aagcaagatt cctgaaacaa gagtaactct tgttgtaatg actataggta tgcttgccaa
     4621 tatagcttca gatgcaggta caatattatt tccaccatta gctgcacttg tatatcttgg
     4681 tgttggtaga catccactta taggtttatt ttctggatat gcagcagtct gtctaggatt
     4741 tgccgcaaac attatgataa gtgtcaatga tatacttgct gcaagtttta cagttccagc
     4801 agctcaaatg attgattctg gatttcaagc caatgcaaca atgaacttat tttttatgat
     4861 agcatcaact tttgttctaa tacttttggc aacatgggta acagaaaaaa ttgtagctcc
     4921 tagatttggt aaatatgaag gaaatgcaga gttagatgtt gatggaagtc ttagcaatgt
     4981 agaaaagaaa ggtcttaaaa aagctggtat aagtatacta atatatgtag ctattataat
     5041 agctcttagt gtaattggta aaaaaccatt cttagctgac ccagaaacag gagatttact
     5101 atcaaacaat gctcctctta tgaaaggtat ggtacctata atagcactag cattttttat
     5161 acctggtgtt gtatatggaa aaactatagg caaaataaag aaggataagg atgtagtctc
     5221 tataatggct agtgcaatga gtgatatggg cggatatata atactagcat ttgctgcttc
     5281 tcaattttta caattattca caaatagtaa tttaggtctg atactagctg taaagggcgg
     5341 agaattttta aaatcagcag gaataaatgg acctttattg ttgataggat ttattatatt
     5401 atcgtgtttt ataaatttat ttataggtag tgcatctgca aagtgggcta ttcttgcacc
     5461 tatatttgtg ccaatgttta tgatggttgg atacaatcca gcacttactc agatggctta
     5521 tcgtataggt gattgtataa caaatccact tagtccatta tttccgtact tccctattat
     5581 actagcattc acaagaaaat acgataaaga tgctggtatg ggtactgtaa ttgctaatat
     5641 gataccatac tctatatcat tcttaatagt gtggactata ctattaatat tgtttatggt
     5701 atttaatatt ccactagggc ctggaatact tccttcatat agcatgtaac ttatttaaat
     5761 ctttctaaaa ggagaaataa tttatgttag ataaaaatag acttataaat aattttatgg
     5821 atatggttag aattgatagc ccatctaatc aagaattaga aatgtctaag tggttggtaa
     5881 actacttaaa agaaagaaat atagatgcca ttatagatga tgcaggtgaa aaatatggtg
     5941 gtaatacagg taatgtcatt gcctatataa aaggtgaaga aggaagtaga ccactttgtt
     6001 tatgtgcaca catggaccaa gttcaacctt gtttaggtgt aaaaccaata ctagatggta
     6061 atgtagttag aagtgatgga actactacac ttggtgcaga tgataaagct ggaatagctg
     6121 ctatacttga agctttagaa catgttatta cagaaaaaat accacataga gatatttatc
     6181 tttgttttac aatctgtgag gaagctggaa tgcatggtgt caaaaacttt aatcctgaca
     6241 acttaccttg taaagatatg gtgattttag attctggtgg agctattggt tcgatagcat
     6301 acaaagctcc tgcccaacaa tctataaaaa tatcttttca tggaaagaaa gcacatgctg
     6361 gtatagaacc tgaaaagggg ttaaatgcta tattagtggc ctcttatgca ataagcaata
     6421 tgcacatagg cagaatagat tcattgacta cttcaaatat aggtaaaata gaaggtggtg
     6481 gagctacaaa catagtaact gataaggtta cattgactgc tgaaattagg tctcatatac
     6541 ctgaaacttt agagtatgag cttaatcata tggaaaaatg ttgtaaggat gctgcatcta
     6601 aatttaatac tacttatact tttgaacata atatgtctta ccctagtttt gaacttagta
     6661 gagatagtca cgttttcaag ttaagtgaag aagcaataag acaagttggt attgttccaa
     6721 atccaatggt aattggtggt ggaagtgacg caaatatatt agctaatctt ggatataatt
     6781 gtgctatatt aagtctaggt atgtatgatg tacacacagt taatgaatat gtaaatatag
     6841 acgaattata tgatactgcc aaaatagttt atcatatgat taaattataa atatcataat
     6901 ataactcttt aaattataaa aataaaagcc tatgtatctt caactttata agttatatat
     6961 taaagttgtt ttacataggt tttagtattt tatataataa tttgttttaa c
//
LOCUS       DAEPXF010000186         6756 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain HB27
            SAMN13376389-rid12479423.denovo.193, whole genome shotgun sequence.
ACCESSION   DAEPXF010000186 DAEPXF010000000
VERSION     DAEPXF010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13376389
            Sequence Read Archive: SRR10518432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6756)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6756)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001069624.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001069624.1
            Genome Coverage       :: 315x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/15/2021 01:54:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,165
            CDSs (total)                      :: 4,076
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 89
            rRNAs                             :: 10, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 10 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 19 of 69
            Pseudo Genes (incomplete)         :: 44 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 10 of 69
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6756
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13376389-rid12479423.denovo.193"
                     /strain="HB27"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="China: Hebei"
                     /collection_date="2017"
     gene            129..1253
                     /locus_tag="KRL67_003269"
     CDS             129..1253
                     /locus_tag="KRL67_003269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3810 domain-containing protein"
                     /protein_id="HBH1524113.1"
                     /translation="MSKKTKYFSLILFPIALLLNFIASKVPNMVEKYYSQFIDKIIVQ
                     ILSKVSGIFPFSLYEITMYIIVISIFLFLCCTLSTIFNKKKKLNIFLKNSILNILSIV
                     SIVYFLFVVLWGINYNRIPLETTLINNYNLENNTSIQSKQHSVKELTKLYKFLVIKAN
                     ETRKLTLQDKNGIVKSNTDYNGVINRAQLGYDNILDILPSVSGSYSKPKYIISSNLMC
                     YTGITGIYFPFTGEANVNIAIPDLYIPCTVEHEMAHQRGFASEDEANFIAYLTSIKHP
                     HIDFNYSGYILALNYTASALSKVDYNAYVDISAGISDSVRRDLKNESKFWQKYEGKID
                     EISNEFNNSYLKANGVTEGTQSYGKMVDLLLTYYELYPYN"
     gene            complement(1727..2551)
                     /locus_tag="KRL67_003270"
     CDS             complement(1727..2551)
                     /locus_tag="KRL67_003270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH1524114.1"
                     /translation="MRYKKISSLPILVVALLITGTTTTFSLSTTNSEKTSVYLASTVS
                     TITRKVSDIQSSKGLVYNENIPLFINNSKIQYDDTPYHWPSNVISLTNSSEKAIMDYE
                     ITCLAYDKNGKPLEIYWDAQNVAADGEVGSVGFSPAGVDYGIVTGISPVSPKSYSHTY
                     RKMQQSPPQDIISMFEKQQGKAWVENWLKEWKQMEKEYAKQNAIAPGKNQNDAFLLFD
                     KWKQSTGEHGVKYIISCVKQVTFNDGSVWKNSAYENWLKSFQGKEVSNSVLENYYK"
     gene            complement(2899..3954)
                     /gene="ispG"
                     /locus_tag="KRL67_003271"
                     /gene_synonym="gcpE"
     CDS             complement(2899..3954)
                     /gene="ispG"
                     /locus_tag="KRL67_003271"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HBH1524115.1"
                     /translation="MSYERRKTREVSVGTVKIGGENPISIQSMTNTDTRDADATIAQI
                     KRLEEAGCDIVRVAVPDIKAAKNIAKIKSSVNIPIIADIHFDYKLALEAIEQGVDGIR
                     INPGNIGSIERVKMVVEKCKERNLKIRIGVNGGSLEKELLKKYGSATTEALVESAMGH
                     IKILEDLDFHNIVISLKSSNIYKTVDAYELISKKVDYPLHIGITESGSVHKGTIKSSI
                     GVGALLLKGIGDTVRISLTGDPVEEVIVGKQILRSLGLLNDKIKVISCPTCGRCNIDL
                     ISVVNEVEEKIGSMEKNITVAIMGCAVNGPGEAREADIGIAGGKGEGLLFKKGEIVRM
                     IDGNKLVDELLEEIEKL"
     gene            complement(4137..5141)
                     /gene="rseP"
                     /locus_tag="KRL67_003272"
     CDS             complement(4137..5141)
                     /gene="rseP"
                     /locus_tag="KRL67_003272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HBH1524116.1"
                     /translation="MTIIAALILFSIIVLIHELGHFIFAKRSGIKVNEFSIGMGPKIY
                     SVKKDTEYSIRALPIGGYVSMEGEDEEQISPNSFGNKSILQRFSTIVAGPIFNIILAA
                     ILLVPVFLYIGSPTTKLGKIMPDTPAQAVGLQVGDKINKINGNSVKTWDEVANIINTS
                     SGGELKLSITRDGSDKVVNVTPKNNNGKYEIGIQPQREKDFLGSIVNACKTTVDMTKQ
                     MLTFLGQMITGRVPGGIGNAVAGPVGVIGMVSDAARTGIINVVYLAAVISLNLGIVNL
                     LPIPALDGWRILMLLLEAVRGGKKLDPNKEGMINVVGFGALMLFMLFITYKDILRLFQ
                     "
     gene            complement(5154..6308)
                     /locus_tag="KRL67_003273"
     CDS             complement(5154..6308)
                     /locus_tag="KRL67_003273"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HBH1524117.1"
                     /translation="MKKISILGSTGSIGKQTLDVVREHSDKFEIVAISANSNIELLLE
                     QIVEFKPKYVTVFEENKALKLKEILPKNIEIEVLAGMEGLKIISSLDEVDILLTAVVG
                     MIGLVPTLCAIKKGIDIALANKETLVTAGELVMKEAEKYNVNILPVDSEHSAIFQCLN
                     GENKKNIEKIILTASGGPFRGKKKGELVNITKNEALKHPNWSMGRKISIDSSTLMNKG
                     LEVIEARWLFGVEQENIDVVVHPQSIIHSMVQYADSSIIAQLGCPDMRLPIQYALTYP
                     DRMESSFERMNFSKFSTLTFEEPDLETFPCLKLAYECLKMGGTYSSVLNSANEVLVSE
                     FLEDKIGFYDIPYYIEKTLEVHSSISEPTLEEILETDRWSRAYVANLIKK"
     gene            complement(6472..6675)
                     /locus_tag="KRL67_003274"
     CDS             complement(6472..6675)
                     /locus_tag="KRL67_003274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBH1524118.1"
                     /translation="MISYAPLWKLLIDRKIKKMEFVNISGISISVLGRLGNDKSVSMD
                     TMEKICLALDCKIEDVVEIKKGI"
BASE COUNT         2232 a         1195 c          707 g         2622 t
ORIGIN      
        1 attaatatat tataacttat catttattta gtataaagtt ataaatagat atatttatac
       61 ttattttaca atctttcttc aattgatata taatatgttt atataaacta atgtatgtga
      121 ggtgaaatat gagcaaaaaa acaaaatatt tttctttaat tctctttcct attgctttgc
      181 ttttaaattt tatagccagc aaagttccaa atatggttga aaaatattac tcccaattta
      241 tagataaaat aattgtacaa atattaagta aagtttcagg aattttccca ttttctctct
      301 atgaaattac aatgtacata atagttatat ctatcttctt atttttatgt tgtactttat
      361 ctacaatttt taataagaaa aaaaaattaa acatattttt aaaaaattct attttaaata
      421 tattatccat tgtatcaata gtatactttc tttttgtagt gctttgggga ataaactata
      481 ataggattcc actagaaacc acacttataa ataactataa tttagaaaac aatacctcta
      541 tccaatcaaa acaacatagt gttaaagagc ttactaaatt atataaattc cttgtaatta
      601 aagcaaatga aactagaaaa ctcacattac aagataaaaa tggtattgta aaatctaata
      661 cagattataa tggtgtcata aatagagctc aactaggata cgataatatc ttggatatat
      721 tacctagtgt tagtggtagt tattctaagc ctaagtatat tatttcatca aatctaatgt
      781 gctatacagg gataactggc atatactttc cttttacagg tgaggcaaat gtaaatatag
      841 caatacctga cttgtatatt ccttgtacag ttgaacatga aatggcacat caaaggggat
      901 ttgctagtga agatgaggct aattttatag cgtatcttac ttctataaaa cacccccata
      961 ttgattttaa ctattctgga tatattttag ctttaaatta tactgcttct gctttaagta
     1021 aggttgatta taatgcatat gttgatattt ctgctggtat ttcagattct gtccgtagag
     1081 atttaaaaaa tgaaagtaaa ttttggcaaa aatatgaagg taaaattgat gagatttcta
     1141 atgagtttaa caattcttac ttaaaagcta atggcgttac tgaaggcact cagagttatg
     1201 gtaaaatggt tgatttattg ctaacttatt atgaattata tccatataac taaacactac
     1261 aattcagaac tttattcata tacagctggt attaaatcag ctgttttcta tttaaataaa
     1321 ttatttagct ccaagcaatc ttatataatt tgaaagtact tatactatct gtataacatt
     1381 tcaattctaa ctattttagt taaaacattt ctattatctt ttttgataaa tgttttaact
     1441 aagattttta aaacataaaa cttccatatt atttcactat ctatataaaa agctaaaaat
     1501 ttacatataa aattacataa ctaaaaaatc ataaatttat agaaaacata tattcaaaat
     1561 aaaaaagcat gtatcaaaac ttgacacatg cttgtaatct tatgtatata cgccattttg
     1621 tcacaataaa aatttccttg taacactgac atttttaaac aaaatagttc ttatttaatt
     1681 aaattaacat tagttaaatt cgtacttatt ttgctatcat taaacattac ttatagtaat
     1741 tttctaatac actatttgaa acttccttcc cttgaaaact tttcaaccaa ttctcatatg
     1801 ctgaattttt ccatacacta ccatcattaa atgttacttg ttttacacaa gatataatgt
     1861 actttacacc atgctctcca gttgattgtt tccatttatc aaacaataga aaggcatcat
     1921 tttgattctt acctggtgct attgcatttt gctttgcata ttctttctcc atttgtttcc
     1981 attctttcaa ccaattttct acccatgcct tgccttgttg tttttcaaac atacttatta
     2041 tatcctgtgg tggtgattgt tgcatttttc tataagtatg tgaatatgat tttggagaca
     2101 caggagatat gcctgtaaca ataccatagt ctacaccagc tgggctaaac ccaacacttc
     2161 ctacttcgcc atctgctgcc acattttgag catcccaata tatctcaagt ggttttccat
     2221 ttttatcata tgctaagcac gtaatttcat aatccataat tgctttttca ctgctatttg
     2281 ttaagcttat aacattgcta ggccaatgat atggagtatc atcatactgt attttgctat
     2341 tatttataaa taatggaata ttttcattgt aaacaagacc tttactactt tgaatatctg
     2401 atactttcct tgtgatagta cttactgtac ttgctaagta tacacttgtt ttttctgaat
     2461 tggtcgtact aagagaaaat gttgttgtcg ttccagtaat cagcaaagca acaactaaaa
     2521 taggtaagct agatattttt ttatatctca cagttcttca ccccttcttc tattaactta
     2581 taataaaaca atttaccaaa acattaaaat ctatgttaac ataatttacc atatgttcta
     2641 gtaactatat cctatcatag ttactatcaa gtttcaatac tattgatgga aattttcact
     2701 atattaaatc tttttttaat tttataaatt tacaatatga tttaactcat acatatatag
     2761 tcaaattaga atataagttt cttacattta atgaagttta taaaataaaa aatgactaga
     2821 taattaaata ctgtaaataa aaactcatta caaaagtatt taaaaatcta gtcaattaat
     2881 atatatctga attgtatact ataatttctc aatctcttct agcaactcat caactaattt
     2941 gttaccatca atcattctca ctatttcacc ctttttaaat aaaagacctt cacctttacc
     3001 cccagcaata cctatatctg cttctctggc ttcaccaggt ccatttacag cacagcccat
     3061 aattgcaaca gtgatatttt tttccatact accaatcttt tcttcaacct catttacaac
     3121 actaattaag tctatattac atctaccaca tgtagggcat gatataactt taatcttatc
     3181 atttaaaagt cccaaacttc ttaaaatttg tttaccaaca ataacctctt caacagggtc
     3241 gcctgttaaa gatattctaa cagtatctcc aattcctttg agaagaagtg ctcctacccc
     3301 tatagaagac tttattgttc ctttgtgaac acttccagat tctgtaattc caatatgaag
     3361 aggataatca actttttttg atattaattc atatgcatca acagttttat aaatattgga
     3421 tgattttaga gatataacta tattatgaaa atctaagtct tcaagaatct taatatgtcc
     3481 cattgcactt tcaactaatg cttcagtagt tgcagagcca tactttttta aaagttcttt
     3541 ctctaaagaa cctccattga ctccaattct aatttttaaa tttctttcct tacatttctc
     3601 aactaccatc ttaactctct ctatacttcc gatattacca ggatttattc ttattccatc
     3661 tacaccttgt tctatagctt caagtgctag cttataatcg aaatgtatat ctgcaataat
     3721 tggtatattt acactagact ttatctttgc tatattttta gcagctttaa tatcaggaac
     3781 agcaactcta actatatcac aaccagcttc ctctagtctc tttatttgag ctattgtagc
     3841 atctgcatct cttgtatcag tatttgtcat agattgtata cttataggat tttctcctcc
     3901 tatttttaca gttcctacac ttacttctct cgtctttctt ctctcgtaac tcatattcca
     3961 cctctatata ttattttata ttgaatctct ttaaatatta aaatattata acctacatgt
     4021 atattataaa ttaaaaagct atctagttaa ataagaataa atttcataca taaaaattag
     4081 tttattctta tttaaaacta catagcctat tttcttaaaa tctctaaaaa actaaatcat
     4141 tgaaataatc ttagtatatc tttatatgtg ataaaaagca taaataacat taaagctcca
     4201 aaaccaacaa catttatcat gccttcttta tttgggtcga gttttttacc acctctaaca
     4261 gcttctagta atagcataag tattctccat ccatcaagtg ctggtattgg taatagattt
     4321 actataccta aatttaagct tatgacagct gctaaataca ctacatttat gatgcctgta
     4381 cgagcagcat ccgataccat accaataaca cctacgggtc ctgctacagc attgccaatc
     4441 cctcctggta cacgtcctgt aatcatttga cctagaaatg ttagcatctg ttttgtcata
     4501 tctacagtag ttttacatgc atttactatt gaccccaaaa agtctttttc tctttgtggt
     4561 tgaatcccta tttcatactt tccattgtta tttttaggtg ttacatttac aactttatca
     4621 cttccatctc tagttatact aagttttaat tctccaccag aagaagtatt tattatattt
     4681 gcaacttcat cccaagtctt aacagaatta ccattaattt tatttatctt atctcctact
     4741 tgaagcccta cagcctgtgc aggagtatct ggcattatct ttcctaattt agtcgttgga
     4801 gaacctatat acaaaaatac tggtactaaa agtattgctg ctaatattat attgaatatt
     4861 ggacctgcta caatagtact aaatctttgt aatatagact tgtttccaaa agaatttgga
     4921 cttatttgct cttcatcttc accttccata cttacatatc caccaattgg aagtgctctt
     4981 atagaatatt ctgtatcttt ttttacacta tatatcttag gccccatacc tatagaaaac
     5041 tcatttactt tgataccact tctttttgca aatataaaat gtcctaactc atgtattaat
     5101 acaattatac taaatagtat taatgcggct attatagtca taaattcacc ttcctatttt
     5161 tttatcagat ttgcaacata agctctactc catctatctg tttctaagat ttcttctaat
     5221 gttggttcac ttatgctact atgaacttct aaagtcttct ctatgtaata tggtatgtca
     5281 tagaaaccaa ttttatcctc taaaaattca cttactaaaa cttcatttgc tgaatttaaa
     5341 acagaagaat aagttcctcc catttttaaa cactcatatg ccaattttaa acatggaaaa
     5401 gtttctaaat ctggttcttc aaaagttaaa gtactaaatt ttgaaaaatt cattctttca
     5461 aagctacttt ccattctatc tggatatgta agagcatact gtattggtag tctcatatct
     5521 ggacatccta attgagcaat tattgagcta tctgcatact gcaccattga atgaattatg
     5581 ctttgtgggt gaactactac atctatattt tcttgttcta ccccaaacaa ccatctagct
     5641 tctatcactt caagtccttt attcataagc gttgaagaat caatacttat ttttcttccc
     5701 atactccaat ttggatgctt caaagcttca tttttagtta tatttacaag ttctcccttt
     5761 ttctttcctc taaatggacc accagatgct gtaagtatta tcttttctat atttttttta
     5821 ttttctccat ttaaacattg aaatatagca ctatgttcac tatcaacagg aagaatattt
     5881 acattgtatt tttctgcttc tttcattaca agttcaccag cagttaccaa cgtttcttta
     5941 ttcgctaatg ctatgtctat tcccttcttt atagcacaaa gagttggaac taatcctatc
     6001 atacctacta cagcagttag aaggatatcc acttcatcaa gtgatgatat tatttttaat
     6061 ccttccattc ctgctaaaac ttctatttct atatttttag gcaatatttc ttttaatttc
     6121 aatgctttat tttcctcaaa aactgttaca tatttaggtt taaactctac tatttgttct
     6181 agcaataatt caatattact gttagctgat attgcaacta tctcaaactt atcactatgt
     6241 tctctaacca catctaaagt ttgtttacct atagagcctg tagaacctaa aattgatatc
     6301 tttttcatat aataatcctt tctaataata aatataccct gtcaaaacat tataacacta
     6361 ttaaaacaat tatttttact attcatagca atttcatata ttattccaca gattaaaatt
     6421 aatctattat tttttgtatt ttgtggaata ttataaaata ttctagtatt attaaatgcc
     6481 cttttttatt tccacaacat cttctatttt acagtctagt gctaaacata tcttctccat
     6541 tgtatccata gaaacagatt tatcatttcc tagtctgcct aaaacagaaa tacttatacc
     6601 tgatatattt acaaattcca ttttctttat ttttctgtca attaacaatt tccataatgg
     6661 agcgtatgaa atcatgttat cacctcgcaa aatatattct atcacatttt atctattttg
     6721 gaaaattttc atctatttag ataaataatt atttta
//
LOCUS       DAEPXF010000187         6749 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain HB27
            SAMN13376389-rid12479423.denovo.194, whole genome shotgun sequence.
ACCESSION   DAEPXF010000187 DAEPXF010000000
VERSION     DAEPXF010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13376389
            Sequence Read Archive: SRR10518432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6749)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6749)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001069624.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001069624.1
            Genome Coverage       :: 315x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/15/2021 01:54:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,165
            CDSs (total)                      :: 4,076
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 89
            rRNAs                             :: 10, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 10 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 19 of 69
            Pseudo Genes (incomplete)         :: 44 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 10 of 69
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6749
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13376389-rid12479423.denovo.194"
                     /strain="HB27"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="China: Hebei"
                     /collection_date="2017"
     gene            1..450
                     /locus_tag="KRL67_003275"
                     /pseudo
     CDS             1..450
                     /locus_tag="KRL67_003275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901484.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            810..1103
                     /locus_tag="KRL67_003276"
     CDS             810..1103
                     /locus_tag="KRL67_003276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH1524119.1"
                     /translation="MNRGNRVIYNQDGKILLQTGEATGDILEHDTITELHYIDVGYGN
                     IDYSKQYIESINPITKEPILKDIPIYLSEEEKRIQELENQLLIAENEKVGGLL"
     gene            1103..1285
                     /locus_tag="KRL67_003277"
     CDS             1103..1285
                     /locus_tag="KRL67_003277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH1524120.1"
                     /translation="MNINNVVVRILAERILNKGLNPLKNREFELDDVTNTEYRKAVED
                     YIIKQSGVVEGIEPTA"
     gene            1318..1884
                     /locus_tag="KRL67_003278"
     CDS             1318..1884
                     /locus_tag="KRL67_003278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH1524121.1"
                     /translation="MTFKELVNKVRNLVLEAKNVTIEDTESKFTSENVEGALKECIDR
                     ADEAFQEADSGKTLLSTAIGSPATPEQTFQDYANYITGFKSNISNLETQLKSKYSIRH
                     GPIDGDDRNPFSANFGKSASYLIVYVYFRRNVYYYNPSGSSLGSSTGGSERAWITIDS
                     NKTGFSVHSYDTNYESYTFTGYYIACFA"
     gene            1915..2205
                     /locus_tag="KRL67_003279"
     CDS             1915..2205
                     /locus_tag="KRL67_003279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH1524122.1"
                     /translation="MEEISINLLCAVAGVVISYLAFRNSSNRKIQDDTETTTKLEQQI
                     TFLCENVRDIKHDVAKFNTSFLDISERVAKVEASTKQAHLRIDEIINRIGGK"
     gene            2209..2466
                     /locus_tag="KRL67_003280"
     CDS             2209..2466
                     /locus_tag="KRL67_003280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HBH1524123.1"
                     /translation="MDNLISFIPEQLLILVAALYVIGAGCKKYKQLDNKYIPVVLLIL
                     GIGFSIWMLGLNPNAVLQGIICWGISIGINQTYKQLKEENK"
     gene            2466..3281
                     /locus_tag="KRL67_003281"
     CDS             2466..3281
                     /locus_tag="KRL67_003281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HBH1524124.1"
                     /translation="MKIGINCGHTKTGPGSGAIGKINESIETRNVGYKVIDKLKKLGN
                     NVVDCTIDKASTQSECLSKITAQANRQDLDWFISIHFNAGGGKGCEVYTYKGKQYQDA
                     IDVCKKISDLGFTNRGVKDGSGLYVVKKTKAKSMLIEVCFVDTEDANKYLSLGADKLA
                     TAIVEAITKHISSAEENNYNRYKHTIVYSGDDKVSADILGLYYKREKESYLVTDIKDY
                     KPHRTQNLYVIGGVTCNKMKEMSKTTGEKFTQLYSNDVWSTMDKAIEFVKEKL"
     gene            3353..4459
                     /locus_tag="KRL67_003282"
     CDS             3353..4459
                     /locus_tag="KRL67_003282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HBH1524125.1"
                     /translation="MLTFLDLFAGIGGFRLGMERAGHKCVGHCEYDKFANLSYKAMHN
                     PKEDEWFEKDIREIRAENIPKADVWCFGFPCQDISIAGNKLGFKGSRSSLFFSVTKII
                     RELKEEDRPKYLLIENVKNLLSVNRGFDFAKLLIELDEIGYDAEWQNLNSKNFRVPQN
                     RDRVYIIGHLRGRSTRKVFPITGTSRKTTLKQINSPSHSSNRIYDSSGLSKCLLSKGG
                     NNTGLYKVGNVNPSGNGIGGQVYNSKGLSPTLTTEKGESPKILIKENTKKGHIEAAIG
                     DGIRLDHIGGKTGRGRVQHKRSCTLTTASNAGVIGKKFRVRRLTPKECFRLQGFPDDY
                     YDRAASVCSETQLYKQAGNSVTVDVVEEIAKKLK"
     gene            4597..4932
                     /locus_tag="KRL67_003283"
     CDS             4597..4932
                     /locus_tag="KRL67_003283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HBH1524126.1"
                     /translation="MLTQSLKRGELSIMKIFWKRSSSISKKEIIRLCNEKYKWRKSTT
                     KILLKRLVKKRVLLKRIKFFHIYYIPIVTEKEYIKCKMNNLEPNYLDGYFVRLMSTAH
                     KFKNIDEED"
     gene            complement(4943..5458)
                     /locus_tag="KRL67_003284"
     CDS             complement(4943..5458)
                     /locus_tag="KRL67_003284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009899650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH1524127.1"
                     /translation="MTWSDLKEKNINEVVDCINNKLSKFESLKKVGDELGANESTIRK
                     WLNKKGYKRTGNKFVEDNKSYDDKYHIDSISSKIDDDIKYLSSEIDNIKSVLEWFKTK
                     DDKCHIDGIKENDISIDLPDQSIKRTTIRVNNTVWGMFNKFADENKHYDKHDLLSQLL
                     LESLLKYVKSR"
     gene            complement(5541..6446)
                     /locus_tag="KRL67_003285"
     CDS             complement(5541..6446)
                     /locus_tag="KRL67_003285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParM/StbA family protein"
                     /protein_id="HBH1524128.1"
                     /translation="MSKLGIDIGNYAVKTSTDDIFESKVTEVKNFGSDSDSIKIGNKT
                     YYLGEGDEEINIVKYEKENFLPLLLGAICRNTDDEVIDLALGLPVKQFGGLRKDLIEK
                     LQGKEYHVEFAKGNETTKRDIVIRSVQVFPEGVTGYLYYAKDIVDQVAGRDVVLVDIG
                     GKTTDIALVQGNKATNPYSINVGTINIYDAIKKSLEMDERFLGKVEIKREKIQDYINK
                     GFYLNGEKQDIKKNIDASISLFKEIYNELKLNYPVSTAAVVVMGGGAKLLGEAFKKNI
                     PGIIVMSDVDKHVFANAKGYKKMMK"
     gene            complement(6466..6669)
                     /locus_tag="KRL67_003286"
     CDS             complement(6466..6669)
                     /locus_tag="KRL67_003286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH1524129.1"
                     /translation="MSRIVLTFKNNDKEKAIEKFLDEKLSATAYLKELVWEKMNEKKD
                     NAVVEQKKEIEDPANNFDFGSLE"
BASE COUNT         2555 a          940 c         1047 g         2207 t
ORIGIN      
        1 ctattaacta aaatagaata ttttatagat gatgtactaa aagaaactat aacaacagat
       61 ttaactcttg aaaaaacaat taactataca ttagaagata atgctctaca cacacttaaa
      121 atagtcgtta cagacagtaa taatgctaca gctgaaaaag tgttaagtat aagtaagaat
      181 ataatgccac tgcccgaaaa tgtaaatttg caagacatat cttctaaact aattgaaatt
      241 aatacaggat ttaaaactgg taaaacaagt attataaaca ctttagcatt aaagaatata
      301 gaagcaagtt taaataatac acttgttgag ttatcagaga aaataaaaac aagttttgat
      361 agttcagacg ctagtgtgca ggagttgcaa aataggataa cagaattgac taatcagtta
      421 agtcaaagga ttaaatatgc aacgggtact tatactatac cagatggcac tagtagttta
      481 gttgttccaa ctaatttaac ttttgtgcca aagactatta tagttaaaat attttctgta
      541 aaagatggtt ctaatccttc taaaactctt agtgcttatc cttgtatgac tggtgtaaat
      601 caaaatctta gatatgacaa tggttcatat actcgcgtta ttggtaatgc gagtattaga
      661 gatgttactg ctgacagttt taagattgaa cttggtaaaa gtgattttaa cgctggggtt
      721 gagtttcctt ttacgtttta tagtaaaact tttagatggt atgcgttaga tatagaattt
      781 ttaaataatt aataatatga ggtgatgata tgaatagagg aaatagagta atttataatc
      841 aagacggaaa gatattactc caaacaggag aagcaacagg ggatatatta gagcatgata
      901 caataacaga attacattat attgatgttg gatacggaaa tatagactat agtaaacagt
      961 atatagaatc tataaatcca ataacaaaag aacctatttt aaaagatatt ccaatctatt
     1021 taagcgaaga agaaaagaga atacaagagt tagaaaatca attactaatt gcagaaaatg
     1081 aaaaagtggg aggattatta taatgaatat aaataatgtt gtagtaagaa tattagcaga
     1141 gagaatatta aacaaaggat taaaccctct aaaaaatcga gaatttgaat tagatgacgt
     1201 aactaacaca gagtatagaa aagcagtaga ggattatata attaaacaga gtggagtagt
     1261 agaaggaata gaaccaacag cgtagtaggt tcttttttta ttgaaagagg tgattaaatg
     1321 acttttaagg agttagttaa taaagttaga aatcttgtat tagaagcaaa gaatgtaact
     1381 atagaagata cagaaagtaa atttacaagt gaaaatgtag aaggagcatt gaaagaatgt
     1441 atagatagag cagatgaggc ttttcaagaa gccgatagtg gaaaaacact tttatcaact
     1501 gctatcggct ctcctgctac accagaacaa acatttcaag attatgcgaa ctatattaca
     1561 ggatttaaga gcaatataag caatttagaa actcaattga aaagcaaata ttctattaga
     1621 catggtccca ttgatggaga tgataggaat cctttttctg ctaattttgg caagagtgca
     1681 agttacctta ttgtctatgt ttactttaga agaaatgtat attattataa tcccagtggt
     1741 agttctttag gaagtagtac aggaggctct gaacgtgcct ggattactat agatagcaat
     1801 aaaactggtt tttcagttca ttcatatgat actaattatg agtcatatac ttttacaggt
     1861 tattatattg cttgtttcgc ataataaatt atattattaa aactaggagg atgtatggaa
     1921 gaaattagca taaatctatt atgtgcagtt gcaggagttg taatatccta cttagcattt
     1981 agaaatagtt caaacagaaa gatacaagat gatacagaaa caactacaaa attagaacaa
     2041 caaataactt ttctgtgtga gaatgtaaga gatataaagc atgatgtagc aaagtttaat
     2101 acaagtttct tagatatcag tgaacgagtt gcaaaagtag aagcaagtac aaaacaagca
     2161 catcttagaa ttgatgaaat tataaataga attggaggaa aataaaagat ggataattta
     2221 ataagtttta taccagagca gttactaatt ttagtagctg ctctttatgt tataggagca
     2281 ggttgcaaga aatataagca attagataat aaatatattc cagtagtgtt attgatactt
     2341 ggtataggtt tctcaatatg gatgctagga ttaaatccta acgcagtctt acagggaata
     2401 atttgttggg gaatatcaat aggtataaat caaacttaca aacaattaaa ggaggaaaac
     2461 aaataatgaa aataggtata aattgtggac atacaaaaac aggaccagga agtggagcta
     2521 taggtaaaat aaatgagtca atagaaacta ggaatgtagg atataaagta attgataaat
     2581 taaaaaaact aggcaataat gtagtcgatt gtactataga taaagcatct actcaatcag
     2641 aatgtttatc taagataaca gcacaagcta atagacaaga tttagattgg ttcatatcaa
     2701 tacatttcaa cgctggaggt ggcaaaggat gtgaagttta cacatataaa ggtaagcagt
     2761 atcaagatgc tatagatgtt tgtaaaaaaa tctctgattt aggatttaca aatcgaggtg
     2821 taaaagatgg aagtggatta tatgtagtaa agaaaacaaa agctaaaagt atgctaatag
     2881 aagtatgctt tgtagacacc gaagatgcaa ataagtattt gagtttagga gctgataaat
     2941 tagctactgc aatagtagaa gctataacta aacatataag ttcagcagaa gaaaacaatt
     3001 ataacagata taaacatact atagtatatt ctggagatga taaagtatca gcagacattt
     3061 taggattata ttataagaga gaaaaagaaa gttacttagt aacagatata aaagactata
     3121 agccacatag aacacaaaat ttgtatgtaa ttggtggagt aacttgtaat aaaatgaaag
     3181 aaatgagtaa gactacagga gaaaaattta ctcaactata tagtaatgat gtatggtcaa
     3241 caatggataa agctatagaa tttgtaaaag aaaaattata aattaaaaga caacctttaa
     3301 aaattaattg ttaataaaac taataaaatt ttcaactcag aaggaacatt atatgttaac
     3361 atttttagat ttgtttgctg gcattggagg ttttaggctg ggaatggaaa gagcaggaca
     3421 caaatgcgta ggccattgcg aatacgataa atttgcaaac ttaagttaca aagcaatgca
     3481 taacccaaag gaggatgaat ggtttgaaaa agacattaga gaaattagag cagaaaatat
     3541 tcccaaagca gatgtgtggt gttttggatt cccatgtcaa gacattagca ttgcagggaa
     3601 caaacttgga ttcaaaggaa gtcgttcaag tttatttttc tcagttacaa aaattattag
     3661 agaactcaaa gaggaagata gacccaagta cttacttatt gaaaacgtta aaaatttact
     3721 tagtgttaat agaggatttg actttgccaa acttctcatt gagttggacg aaatcggcta
     3781 tgatgcagaa tggcaaaatc tcaattctaa aaacttcaga gttccccaaa atagagacag
     3841 agtgtacatt attggacatc ttagaggcag aagtacaaga aaagtatttc ctatcacagg
     3901 aacgagtaga aaaactactc ttaagcaaat aaatagtcct agccatagtt caaatagaat
     3961 ttatgattca agcggattat caaagtgttt attaagtaaa ggtggaaata acacaggtct
     4021 ttataaagtt ggtaatgtca atcctagcgg aaatggtata ggaggacaag tatataatag
     4081 taaaggatta tctccaaccc ttacaactga aaaaggagaa agcccaaaga tattgattaa
     4141 agaaaatact aaaaaaggac atattgaggc agctatagga gatggcataa gactagatca
     4201 tataggaggc aaaacggggc gtggaagagt tcaacacaaa agaagttgta ctttgactac
     4261 tgcaagtaat gctggcgtaa ttggtaaaaa atttagagta aggaggttga ctccaaaaga
     4321 atgttttagg ttacaaggat ttccagacga ttattacgat agagcggcaa gtgtctgttc
     4381 agaaactcaa ctatacaagc aagcaggcaa tagtgttact gtagatgttg ttgaagaaat
     4441 agcaaagaaa ttaaaataga atattaatac aatgcaaaat gtggtaggtt tttccttcca
     4501 ccctttgcat tgtattagct tttttggaat aacttacctg tactatctta aataaaaaaa
     4561 tgctactata ttataaacat ttacaaggag tgcatcatgt taacacaaag cttaaaacgt
     4621 ggcgaattaa gtataatgaa aatcttttgg aaaagaagtt caagcatatc aaagaaagaa
     4681 attattcgcc tttgcaatga aaaatacaag tggcggaaat ctacaacaaa aattctactt
     4741 aaaagacttg ttaaaaaaag agtcttgctt aaaagaatta aatttttcca catatattac
     4801 atacctatag ttacagaaaa ggaatacatt aaatgtaaaa tgaataattt agagccaaat
     4861 tatctggatg gatactttgt acgattaatg tctactgcac ataaattcaa aaatatagat
     4921 gaagaagatt aaatatctcc cttcatctgc tcttcacata ttttaataga ctttcaagca
     4981 ataattgact taacaaatca tgtttatcat aatgtttatt ctcatctgca aatttattaa
     5041 acattcccca cactgtatta tttaccctaa ttgtagttct ttttatagac tgatctggaa
     5101 gatctataga tatatcattt tctttaatgc catctatatg acatttatca tctttagttt
     5161 taaaccattc tagcacactt tttatattat caatttcact agataaatat ttaatatcgt
     5221 catcaatctt actgctaatg ctatctatat gatatttatc atcataactt ttattatctt
     5281 ctacaaactt gtttccagtt cttttatatc ctttcttatt aagccacttc cttatagtac
     5341 tttcatttgc ccctaattca tctccaactt ttttaagact ttcaaattta cttaatttat
     5401 tattaataca atcaacaact tcattaatat ttttttcttt caaatcactc caagtcatat
     5461 aaatcctcct caaaaaaata atagattaca aatatcatat atttgtaatc tattatacat
     5521 actataaact aaatcctttt ttatttcatc atttttttgt atccttttgc atttgcaaaa
     5581 acatgtttat ctacatcact cataactatt atgccaggta tattcttttt aaatgcttca
     5641 cctaacaatt tagcacctcc acccataaca acaaccgcag ctgtactaac tggataattt
     5701 aatttcaatt cattgtatat ttctttaaat agagaaatag atgcatctat atttttcttt
     5761 atatcttgtt tttcaccatt taggtaaaat cctttattta tgtagtcttg tattttttct
     5821 ctttttatct caactttacc taaaaatctt tcgtccatct caagagattt ctttattgca
     5881 tcatatatat ttattgttcc cacatttata gaatatggat tagttgcttt atttccttgt
     5941 acaagtgcaa tatctgttgt ctttccacca atatcaacta aaacaacatc tcttcctgca
     6001 acttggtcaa ctatatcttt tgcataatat aaatagcctg taacgccctc tgggaacact
     6061 tgaacagatc ttattactat atctctttta gtagtttcat ttccttttgc aaattcaaca
     6121 tgatattctt ttccttgtaa tttttcaatc aaatcttttc ttaatcctcc aaattgcttc
     6181 actggtagac ctaatgctag atcaataact tcatcatctg tatttctgca tatagcccca
     6241 agtaaaagcg gtaagaagtt ttctttctca tatttaacta tatttatttc ttcgtcacct
     6301 tcgcctaaat aatatgtttt attacctatt ttaatgctat ctgaatctga accaaagttt
     6361 tttacctctg taactttact ttcaaaaata tcatctgtac ttgttttaac tgcataattt
     6421 ccaatatcta tacctaattt actcatttat gttatttccc ccttattatt ctaaacttcc
     6481 aaaatcaaaa ttgttagctg gatcttctat ttcttttttc tgttcaacta cagcattatc
     6541 ctttttttca ttcatttttt cccaaacaag ctcttttaaa taagccgtag ctgataattt
     6601 ctcatctaaa aacttttcaa ttgctttttc tttatcattg tttttaaatg ttagaactat
     6661 cctactcatt caaccacttc cttttttgta aaatattata aaatttatta atatttacat
     6721 atattttacc aaattttata cagtaattc
//
LOCUS       DAEPXF010000188         6745 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain HB27
            SAMN13376389-rid12479423.denovo.195, whole genome shotgun sequence.
ACCESSION   DAEPXF010000188 DAEPXF010000000
VERSION     DAEPXF010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13376389
            Sequence Read Archive: SRR10518432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6745)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6745)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001069624.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001069624.1
            Genome Coverage       :: 315x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/15/2021 01:54:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,165
            CDSs (total)                      :: 4,076
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 89
            rRNAs                             :: 10, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 10 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 19 of 69
            Pseudo Genes (incomplete)         :: 44 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 10 of 69
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6745
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13376389-rid12479423.denovo.195"
                     /strain="HB27"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="China: Hebei"
                     /collection_date="2017"
     gene            complement(561..1313)
                     /locus_tag="KRL67_003287"
     CDS             complement(561..1313)
                     /locus_tag="KRL67_003287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TSUP family transporter"
                     /protein_id="HBH1524130.1"
                     /translation="MFAIIFLCIGGFLAAFVDSIAGGGGLISMPVLMAIGVPVHLAIG
                     TNKFAASAGCISSAYRYAKSGKINNNLLKKLVPFTIAGSILGVRCVLSISEDILNVLV
                     VVMILIVAIYTFVSKNLGQEDNFESINKKNLRLGMLMALIMGFYDGFFGPGTGTFLTF
                     GFIKIYGYDFLHASANTKILNLTSNITSLLLFMINGQVDYKIAIVFALVMIMGAYVGA
                     KVAIKKGSKMIKPIFLVMALFMVVKLVYQTLV"
     gene            complement(1374..3029)
                     /locus_tag="KRL67_003288"
     CDS             complement(1374..3029)
                     /locus_tag="KRL67_003288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PocR ligand-binding domain-containing protein"
                     /protein_id="HBH1524131.1"
                     /translation="MVDFIESNNEVQKFDWGEVMWIHEPSKTQFNRLSAGIVRFFPGN
                     CQEKHFHLSEEQLLYVIQGEGIQIIDGKKVNIKETSIVYCPPYSEHEIINTGKFDLVI
                     LITYVPHKFSSLRQIPIVFSENNIQELVNVNIIENLANQISNILKLRISVYDSKYKEI
                     FEPKKENKFCDICKSIKPCTKKLVKSINNDNFEKVYHCEYGVSSLEIPIVLEENIVGY
                     IECGNFIVYKSIDVDKNLSELSSSSGIDIKYINKIYNEFPLNPKSRLYVLKENLLMMS
                     EFIQEIAKRNFFENQLSIKDEEILKIRKENIRLEEALKKANSKLLEEEYIINPIDMFN
                     NNLKEYPFELELYIEKEIKNMNLEGVCNLIDTNKLIYSDVRDIIQEMIFVLSRAVLRD
                     LEDLKLISYLRNKYNKKISLVDSDEDLWNVLFEFSKECIDKNRAFWRKDKGNLIENIN
                     EYIQKYYKENISLNSISEVFFISPNYLSSIFNERNKVSITEYINLLRIEESKKYLLDI
                     SFSISDICKKVGFNNSSYFSQIFKKFNNVTPNEYRKNILDNKH"
     gene            complement(3258..3626)
                     /locus_tag="KRL67_003289"
     CDS             complement(3258..3626)
                     /locus_tag="KRL67_003289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HBH1524132.1"
                     /translation="MKYSITDVSKITKISSSAIRFYEEKGLLLPINRKPSGVRYFSEG
                     DVERLIFIKELKNTGLSIKDIHECFHFCDMGDGTIDKRIQLFQQHKQNIKNKIHELEN
                     CMDKIDEKIYYLKEKKMCEK"
     gene            3775..4575
                     /locus_tag="KRL67_003290"
     CDS             3775..4575
                     /locus_tag="KRL67_003290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,4-dihydroxyhept-2-ene-1,7-dioic acid aldolase"
                     /protein_id="HBH1524133.1"
                     /translation="MEKYATMIKEKLNEGKILCGVAVSLSDSVVSELIGISGYDFIWI
                     EGEHGALDRKDIQQHMIAAHAGGAASLVRLVNNDPDLVKPIMDMGTDIICFPFINTVQ
                     DAQKAVAACTYPPNGIRGYNPQRASYYGDMNYFDYVKNANNETLKMMLIEQKAGYDNL
                     EEIVKVKGIDIIALGPGDLSLDMGFSGYMDRIEIKDMISHAAEICKKYKMPFIIFPTI
                     EKKSIEEWVNIGANMLCFAKILPLSLTLSINYYLFIMRLYQFKNNIKL"
     gene            complement(4723..5151)
                     /locus_tag="KRL67_003291"
     CDS             complement(4723..5151)
                     /locus_tag="KRL67_003291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HBH1524134.1"
                     /translation="MSKIYIVYWSGTGNTEKMANFVAEGVKLKGKTPEVLDVSLLKPS
                     DLKEEDKFALGCPSMGAEQLEEGYMEPFVSELESMVSGKQIGLFGSYGWGNCEWMRDW
                     EERMQNAGATIIGGEGITAMEDPDEEAKDECIELGKMLAE"
     gene            complement(5636..6574)
                     /locus_tag="KRL67_003292"
     CDS             complement(5636..6574)
                     /locus_tag="KRL67_003292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family peptidase"
                     /protein_id="HBH1524135.1"
                     /translation="MNKRMKLIPYEINENLRGAKNKFPYGIKQMNARGMWDEGYTGKN
                     IVVGIIDTGCDISHPLLKGKIIGGANFSDDSNGNKNIYEDFNGHGTHVAGIIAASNYN
                     NEVMGVAPDCKLLIAKALNKDGTGTYQSIINAINFAVNNKVDIISMSLGGNKDDKNLK
                     NAVIQAVKNNISVVCAAGNNGDGDSSTSEYSYPASYAEVIEVGAINENYLVEKFSNSN
                     TTIDLVAPGRNIISTYMDNKLAIMSGTSMSAPYVSGSLALIKEWARDEFERDLDEAEL
                     YAQLIKCTRALGMPRTEQGNGYLYLNLYKYKNNSKR"
BASE COUNT         2284 a         1102 c          673 g         2686 t
ORIGIN      
        1 atttattatt ataataacaa aagaataaaa aaattaaatt agcataacct tgttaaatat
       61 agatttgaat gtgaatttaa agttatgtaa aattaaaaat acaggaatga aaattccatt
      121 cctgtataaa atctaacttt tgagtctcag tacaatctta ttttgagaca atcttttttg
      181 ttttatattt tcttatattt aaaatataac gcatatctta tgcaacatat tcatattttt
      241 ctttatattc tttcatcgca tcattccaag cgtcttgtac tgttacacct cttttattag
      301 caattttttg tgccagttca attatcttca tagctcttct tgatatgttc ataaacattc
      361 ctcctacaaa ttaccatata tttcatatat atatcaaggt tatattttta tgatatataa
      421 tccttctatt gtaagattac aatttatatg tgtattatat atgaactata ttagaagata
      481 attagaattt ttataaagct atatgattag attaaatata aaaggtttac aattacttat
      541 aaatttaaaa gggctataag ctaaactaat gtttgatata ccagttttac gaccataaac
      601 aatgccatta ctaaaaatat tggttttatc atctttgaac ctttctttat agcaacttta
      661 gcacctacat aagctcccat tatcatcact aatgcaaaca ctatagcaat tttataatct
      721 acttgaccat ttatcatgaa taataacaat gaagttatat tacttgttaa gtttaatatt
      781 tttgtatttg cagaagcatg taaaaaatca tatccatata tttttataaa tccaaaagtc
      841 aaaaaagttc ctgtacctgg accaaaaaaa ccatcataaa aacccattat aagagccata
      901 agcataccta atcttaaatt tttcttatta atagattcaa aattatcttc ttgacccaaa
      961 tttttagaaa caaaggtgta aatagccact attaaaatca taactacaac caaaacattt
     1021 aatatatctt cacttattga taatacacat ctcacaccta aaatactacc agcaatagta
     1081 aatggtacta atttttttaa aaggttatta ttgatttttc cagactttgc gtatctataa
     1141 gcactactta tacatcctgc tgatgctgca aatttattcg tgccaatagc aagatgtact
     1201 ggcactccaa tagccataag aacaggcata cttataagtc ctcctcctcc tgctatagaa
     1261 tctacaaaag cagctaaaaa ccctcctata cataaaaaaa taattgcaaa cataaaaatc
     1321 ctccatcatt ctctttcaat ttttcttaca atacatatgt tatatgcttt tatttaatgt
     1381 ttattatcta gtatattctt tctatattca tttggagtta cattattaaa tttcttaaat
     1441 atctgtgaaa aataactact attattaaaa cctactttct tacaaatatc actaatacta
     1501 aaacttatat ctaacaaata ctttttagat tcttctatcc taagaagatt tatatactca
     1561 gttattgaaa ccttatttct ttcattaaaa atagaactca aataattggg gcttataaaa
     1621 aagacttcag atatactatt taaacttata ttttctttat aatatttttg tatatattcg
     1681 tttatatttt caattagatt tcctttatct tttctccaaa atgctctatt cttatcaata
     1741 cactccttgg aaaattcaaa taatacattc cataaatctt catcactgtc tacaagtgat
     1801 atttttttat tatatttatt tcttaaataa gatataagtt ttaaatcttc taaatctcta
     1861 agtactgctc tggataaaac aaatatcatt tcctgaatga tatctctaac atcactatat
     1921 attaatttat ttgtatctat tagattacaa actccttcta aattcatatt tttaatttct
     1981 ttttcaatat aaagttctaa ttcaaatgga tattctttta aattattatt gaacatatct
     2041 attggattaa taatgtactc ttcttctaat aatttactat ttgctttttt aagtgcttct
     2101 tcaagcctta tattttcttt tctaattttc aatatttcct catcttttat actaagttga
     2161 ttttcaaaga aattcctctt agctatttcc tgaataaatt cagacatcat taataaattt
     2221 tcttttaata catataatct acttttagga tttaatggaa attcattgta aattttgttt
     2281 atgtatttta tatcaatacc ggaactactt gataattcag ataaattttt atctacatca
     2341 atagacttat atactataaa attaccacat tctatatatc caactatatt ttcttctaat
     2401 actataggta tctctaaact ggaaacacca tactcacaat ggtacacttt ttcaaaatta
     2461 tcattattta tgcttttaac aagtttttta gtacacggtt ttatactttt acaaatatcg
     2521 caaaatttat tttctttctt aggctcaaat atttctttat actttgagtc ataaacagat
     2581 attctaagct tcaatatatt agatatttga tttgcaagat tttcaattat gtttacatta
     2641 actagctctt gtatattgtt ctcagaaaat actatgggta tttgtctcaa actcgaaaat
     2701 ttatgaggaa cataagtaat taatattact aaatcaaatt taccagtgtt tataatttca
     2761 tgttctgaat atggtgggca ataaactata gatgtttcct ttatattgac ctttttacca
     2821 tcaattattt gtattccttc tccctgaatc acatataaaa gctgttcttc acttaaatga
     2881 aaatgctttt cttggcagtt tcctggaaaa aatcttacaa ttccagcact aagtctattg
     2941 aattgtgttt ttgatggttc gtgtatccac ataacctctc cccaatcaaa tttttgtact
     3001 tcattatttg actcaataaa atccaccata aactcacctc gtagtttttg tctaaatatt
     3061 tttttattcc tttaaatatc tccttataat atacattatc ataattttct tttttattgt
     3121 atagataaat agttatatag aacattgtcc aattcttcct aaaaatatgt tgtaaaaaat
     3181 ctttcctcaa atgtttccaa ttgaatttac tctttatatt agaaaaatca aaaaattatt
     3241 tctttattct ataatgatta tttttcacac atcttttttt cttttaagta atatattttc
     3301 tcatctattt tatccataca attttctagt tcatgtattt tatttttaat attttgctta
     3361 tgttgttgaa ataattggat tcttttatct attgtaccat ctcccatatc acaaaaatga
     3421 aagcattcat ggatatcttt aatagataaa ccagtatttt ttaattcttt gataaatatt
     3481 agacgctcta catctccttc agagaaatag cgaacaccac ttggttttct attgatagga
     3541 agtaataaac ctttttcttc ataaaatcga atagcagatg aagaaatttt tgttattttt
     3601 gatacatcag ttatagaata tttcataatc tttacctccc taaattttat atgaataaga
     3661 atattataaa aataccttat acagtagtca acaaaaaagt attgacttca agttaggttg
     3721 aagttgtact ctttgaagtg taaaagaaaa cttacaaaag aaaggatgat ataaatggaa
     3781 aaatatgcaa ctatgattaa agaaaagcta aatgaaggaa agatcttatg tggagtagct
     3841 gtttcactgt ctgattcggt tgtaagtgag ttaatcggta tatctggata tgattttatt
     3901 tggattgaag gagaacatgg tgctttagac cgtaaagaca ttcagcaaca tatgatagct
     3961 gcacatgctg gtggcgctgc ttctttggta cgattagtaa acaatgaccc tgaccttgtt
     4021 aagcccataa tggatatggg aacagatatt atttgtttcc catttattaa tacagtacaa
     4081 gatgcacaaa aagccgtagc agcttgtact tatcctccaa acggtataag aggatacaat
     4141 cctcaaagag cctcttatta tggggatatg aattattttg actatgtcaa aaacgcaaac
     4201 aatgaaacat taaaaatgat gttaattgaa cagaaagcag gttatgataa tcttgaagaa
     4261 atagtaaaag tcaagggtat tgatattata gcattaggcc ctggtgactt atccttagac
     4321 atgggatttt ccggatatat ggatagaatt gaaataaaag atatgatttc tcatgctgct
     4381 gaaatatgca aaaaatataa gatgcccttt attatttttc ctacaataga aaagaaaagt
     4441 attgaagaat gggttaatat tggagcaaat atgctttgtt ttgccaagat actaccttta
     4501 tcacttacgt tatcaatcaa ttattatctt tttataatga gattgtacca attcaaaaac
     4561 aacattaaat tataaatcta attttatact aaaagagact aatattttat ctatctctat
     4621 taaggatatt ttcttatgta tcataatatt ttagtctctc ttagtattat atactgctat
     4681 catcattagt tgctatttat gctattttta taatatacaa atttattcag ctaacatttt
     4741 gcctaattct atacactcat cttttgcttc ttcatctggg tcttccattg ctgtaattcc
     4801 ttctccacca ataattgtgg caccagcatt ttgcatacgt tcttcccaat ctctcatcca
     4861 ttcacaattt ccccatccat atgaaccaaa taatccaatc tgtttacctg ataccataga
     4921 ttctaattct gaaacaaatg gctccatata cccctcttct agttgctctg ctcccataga
     4981 tgggcaacct aatgcaaatt tatcttcttc ttttaaatca cttggtttta gtaagctaac
     5041 atctaaaact tctggtgtct taccttttaa cttcacacct tcagctacaa aatttgccat
     5101 tttttcagta tttcctgttc cactccaata aacaatatat attttactca tttttacctc
     5161 ctgtagtatt ttttaattaa tcaatagtta gttatagcta actaacatct aaaaaacaaa
     5221 aaattccttt atctgaaatc tctttatttt tctaaatata gaatatcata attgattttg
     5281 aaattcaata tttattgata atatatatca tttatatttt atatttctta tatatctcaa
     5341 aacattgtat cgtatttcta atctttcata gaaaaatttc taacttatag tacttttgca
     5401 tctcttataa atcatcttat atatctttta tatgtataag tacatagaaa aacacacctt
     5461 tcccagatgt gctatctata atatcgtata tttatttttc aattctgact agctaatatg
     5521 tttataaatt ttattactta atacctttat acattgatgt aaaatcttac acctaagtaa
     5581 ttttttattt gtctcaaccc tatataacat agctatttta tcatcaaatc aaaaattatc
     5641 ttttgctatt attcttgtat ttataaagat ttaaatataa gtatccattt ccttgttctg
     5701 ttctaggcat tccaagtgct ctcgtacatt ttattaattg tgcatacagt tcagcttcat
     5761 ctaaatctct ttcaaactca tctcttgccc attctttaat tagtgctagt gaccctgata
     5821 cgtatggtgc actcatacta gtaccactca taatagcaag tttattatcc atatatgttg
     5881 atataatatt tcttcctgga gctaccaaat ctattgtagt atttgaatta ctaaactttt
     5941 caaccaaata gttttcattt attgcaccta cttctattac ctcagcataa ctggctggat
     6001 aactatactc acttgtacta gaatcaccat ctccattatt acctgctgca cacactacag
     6061 aaatattatt ttttactgct tgtatgacag cattttttaa attcttatca tctttgtttc
     6121 ctccaagaga catagatata atatcaacct tgttatttac agcaaagtta atagcattaa
     6181 ttatactttg atatgttcca gtaccatctt tatttaatgc ttttgctatt aataatttac
     6241 aatctggagc tactcccata acttcattat tataattaga tgcagctata atacccgcca
     6301 catgagttcc atgaccatta aaatcctcat atatattttt atttccatta ctgtcatcac
     6361 taaaatttgc accaccaatt atttttcctt ttaaaagagg atgggatata tcacaacctg
     6421 tatctattat accaactaca atatttttac cagtataacc ttcatcccac attcccctag
     6481 cattcatttg ttttattcca tatgggaatt tattttttgc acctcttaga ttctcattta
     6541 tttcatacgg aattagtttc attcttttat tcatcatata tcctcctaaa aattttattg
     6601 cttataaaaa tcataaattt tattttataa taggtttata aatcaattca aaacctatat
     6661 ctgcttattt atatttatga tatatttttt atttttagga gtcatatttt attgtatata
     6721 aaataatatt tagataacta tatta
//
LOCUS       DAEPXF010000189         6662 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain HB27
            SAMN13376389-rid12479423.denovo.196, whole genome shotgun sequence.
ACCESSION   DAEPXF010000189 DAEPXF010000000
VERSION     DAEPXF010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13376389
            Sequence Read Archive: SRR10518432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6662)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6662)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001069624.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001069624.1
            Genome Coverage       :: 315x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/15/2021 01:54:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,165
            CDSs (total)                      :: 4,076
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 89
            rRNAs                             :: 10, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 10 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 19 of 69
            Pseudo Genes (incomplete)         :: 44 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 10 of 69
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6662
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13376389-rid12479423.denovo.196"
                     /strain="HB27"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="China: Hebei"
                     /collection_date="2017"
     gene            complement(175..1065)
                     /gene="metA"
                     /locus_tag="KRL67_003293"
     CDS             complement(175..1065)
                     /gene="metA"
                     /locus_tag="KRL67_003293"
                     /EC_number="2.3.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-succinyltransferase"
                     /protein_id="HBH1524136.1"
                     /translation="MALILPKGLPVINKLLDEGIDVIYKEDFKKELKCEENIDTKIAI
                     LNLMPIKIDTELDLLRRIDKTGFNVSVEFIKISTRESKRSCNEYVKEFYKTFDEAKGE
                     YFDGFIITGAPVEQMEFEEVDYWNELEEIMDYSKRKTKSTLYICWAAQASLYKYYNVK
                     KLPLSQKCFGVFKHKVDKNSKIVDGFENEFFAPHSRHTTVNIEALKENKELSIVSHSK
                     EAGPYIITNSRDVFVMGHSEYDKYTLDKEYKRDINKGDKISIPQNYYINDDPSEEPTV
                     KWKKHSELLFRNWIKNYLIQ"
     gene            complement(1095..2360)
                     /locus_tag="KRL67_003294"
     CDS             complement(1095..2360)
                     /locus_tag="KRL67_003294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylhomoserine
                     aminocarboxypropyltransferase/cysteine synthase"
                     /protein_id="HBH1524137.1"
                     /translation="MYNKETICVQGNYKPGNGEPRVLPLYQSTTFKYSSIDQLAELFD
                     LKVDGHIYSRISNPTIQAFEEKISLLEGGVSSVAVSSGQSANMLAVLNICKSGDSILC
                     SSKVYGGTFNLLGPSLKKFGIDLISFDLDSSEDEIVELAKENTKVVFAETLANPTLEV
                     IDFEKIANVAKRINVPFIVDNSLASPVLCNPLKYGANIVTHSTTKYLDGHASSVGGII
                     VDGGNFNWDNGKFPELVEPDPTYHGISYTQKFGNAAYATKARVQLLRDYGNCLSPFNA
                     YLTNLNVETLHLRMERHSENALKIARFLEKHENVDWINYPGLEDNKYYENAKKYLSKG
                     CSGVLSFGVRGGLENAKKFVEKLQIASLVTHVSDVRTCVIHPASTTHRQLTEEQLIAS
                     GVLPSLIRLSVGIENAEDLIADLNQALNF"
     regulatory      complement(2470..2572)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            complement(2626..5283)
                     /locus_tag="KRL67_003295"
     CDS             complement(2626..5283)
                     /locus_tag="KRL67_003295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HBH1524138.1"
                     /translation="MENHKQMPWHSRATHEILESLDSSEEGLTDTEAEKRLKENGLNE
                     LRKKPPRTTLQMLWSQITDAMVMILIGAAILSLIFGEFTEAFVILIIVTVNAVIGIVQ
                     EKKAESSLEALKNMNSPTARVMRDGEENVIPASNLVVGDIVFLEDGAMVPADLRLIET
                     SSLKIQEASLTGESVPSEKDADTILPKECVLGDRSNMAYTSSIVTYGRGVGVVVATGM
                     DTEVGNIAYLLDSQDDFDTPIKRKLNTVGKTLSVIGIIVCVVIFAIGAFYQRPLLPMF
                     MIAISLAISIIPEGLPATATIVMALGVQRMAKRNALIRKLPAVETLGGATVICCDKTG
                     TLTLNKMTVTHIAVNGDFESGKVTSIDTASEKHPLVYKEIVYAGALCNDACINPDKTD
                     EILGDPTEGALIFMAKKFGIDQEALEEEYPRVFEQPFDSDRKCMTTVHNIDEKIIAYT
                     KGAVDEILDLCTKILTSKGERNITEVDKKNIHELCLNMSKGALRVLGFAKREISSIPK
                     EDGENIEYDLTFIGMVGMIDPPRTEVIDAVSTCKEAGIRTIMITGDHKVTATTIAHEL
                     GIWSEENTVISGDELDNLSDDELDEAVKNTTVFARVSPFDKLRIIQSLKRIGEVPAMT
                     GDGVNDSSALKSADIGIAMGISGTDVAKDSSDMILMDDSFTTIAYAIKEGRRVYRNIQ
                     KVIQFLLVGNIAEILTLFVAILLNWDTPLLAVHILWVNLATATLPALALGVDPPSRNI
                     MKHQPVKSTTLFEKNLIQRVVTQGIFVAIMTILAYKIGLNIENHSVGQTMAFCVLAFS
                     QMLRAFSQRSNTDSMFNRNNGKNPFLLISFLTSALLIAVILFVPVFRNAFNLTILNNL
                     EWLITIVLAIMSVIQVEVGKIIKRKKLKK"
     gene            complement(5411..6154)
                     /gene="yaaA"
                     /locus_tag="KRL67_003296"
     CDS             complement(5411..6154)
                     /gene="yaaA"
                     /locus_tag="KRL67_003296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide stress protein YaaA"
                     /protein_id="HBH1524139.1"
                     /translation="MIIMLSPAKNMKNIEVFDRDLSLPCFIDNTKEIVENIKTFGVED
                     FKNKMKINEKLAVLNKNRFESIKFDRLGSPAILTYDGIQYKNIEAENFTRQDEEFANS
                     CIRIISGLYGVVKPYDSIYEYRLEMQTKLRVGEFKNLYEYWGNRIYKELIKEKTAIVN
                     LSSNEYSKSIEKFIKDSDTYITCTFKVNKNGIFKVESTQAKKARGMMAKYIVKNRIRD
                     IEELKKFNLEGYKYQENLSNNSEYIFVKE"
     gene            complement(6164..6628)
                     /gene="bcp"
                     /locus_tag="KRL67_003297"
     CDS             complement(6164..6628)
                     /gene="bcp"
                     /locus_tag="KRL67_003297"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002586316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-dependent thiol peroxidase"
                     /protein_id="HBH1524140.1"
                     /translation="MLSIGTKAPEFTLEDKDGNKVSISDFKGKKVVVYFYPKDNTPGC
                     TRQACAFRNAYDGFKKEDVQVIGISKDSIKSHQKFAEKHELPFILLSDPDLVAIKAFD
                     VWKEKKMYGKTALGVVRATYIIDENGIIEKVFEKAKPDTNAQEILEYLEKQE"
BASE COUNT         2049 a         1233 c          794 g         2586 t
ORIGIN      
        1 ccatatataa aattaaaact acctttatct acaattaaag ttatttctat aaaatataaa
       61 actactatta tagggttaaa ttttaaaaaa ataaggtata aagtatcatt aagattaact
      121 ttatacctat aaatttctat atttttttag ttaacattta tatactctta aaatttattg
      181 tatcaaatag ttttttatcc aatttctaaa caacaattct gaatgttttt tccattttac
      241 agttggctct tctgaagggt catcattaat ataataattt tgaggtatcg atattttatc
      301 tcctttattt atatccctct tatattcttt gtcaagagtg tatttgtcat actcgctatg
      361 acccattacg aatacatctc ttgaatttgt aattatatag ggaccagctt ctttagaatg
      421 actcacaatg ctcaactctt tattctcttt cagagcttca atatttacag tagtatgtct
      481 tgaatgtgga gcaaaaaatt cattttcaaa tccatctact attttactat ttttatctac
      541 cttatgctta aatactccaa aacatttttg acttagtgga agttttttta cattgtagta
      601 tttgtaaaga cttgcttgag ctgcccaaca tatatataat gtagatttag tttttctctt
      661 agaataatcc ataatttctt ctagttcatt ccaataatca acttcttcaa actccatttg
      721 ttcaactgga gctcctgtta ttataaagcc atcaaaatac tctcctttgg cttcatcaaa
      781 tgttttataa aattctttaa catattcatt gcaacttctt ttactttctc ttgttgaaat
      841 tttaataaat tccacagaca cattgaatcc tgttttatct atccttctta gtaaatctaa
      901 ttctgtatct atctttatag gcattagatt tagtatagct atcttagtgt ctatgttttc
      961 ttcacacttt aattcctttt taaaatcttc tttataaatt acatctattc cttcatctaa
     1021 taatttatta ataacaggta atccctttgg tagtattaaa gccatatcat ttcacttctc
     1081 tttctcttag aatattagaa atttaaagct tgatttaaat cagctattaa atcctctgca
     1141 ttttctattc caacagataa tcttattagt gaaggcaata ctccagatgc aattaattgt
     1201 tcttctgtta attgtctatg agtagttgaa gctggatgta taacacaagt tcttacatct
     1261 gaaacatgtg taaccaaaga tgctatctgt agtttttcca caaatttttt ggcattttct
     1321 aacccacctc ttactccaaa tgataaaaca ccactacatc cttttgataa atactttttg
     1381 gcattctcat aatacttgtt atcttcaagt cctgggtaat taatccaatc tacattttca
     1441 tgtttttcta aaaatctagc tattttaagt gcattttcac tatgtctctc cattctaaga
     1501 tgtagtgttt caacatttaa attagtaaga tacgcattga atgggcttaa acaatttcca
     1561 tagtctctaa gcaactgaac tcttgcttta gttgcatatg cagcatttcc aaatttttga
     1621 gtatagctta ttccatgata tgttgggtct ggctcaacta attctggaaa ttttccatta
     1681 tcccaattaa agtttccacc atccactata attcctccaa cacttgaagc atgcccatct
     1741 aaatattttg tggtagaatg agtaactata tttgctccat actttaaagg gttgcaaagc
     1801 actggagatg ctaatgaatt atcaacaata aatggaacat taattctctt agctacattt
     1861 gctatttttt caaaatctat gacttcaaga gttggatttg caagtgtttc tgcaaacaca
     1921 accttagtat tttcctttgc aagttctact atctcatctt cacttgaatc taagtcaaac
     1981 gatattaaat ctataccaaa ttttttaaga ctaggtccta gtaaattgaa tgttcctcca
     2041 tatacttttg aggaacaaag tatactatct cctgatttac atatatttaa aactgccaac
     2101 atatttgcgg attgccctga tgatacagct acagaagata ctccaccctc tagtaaactt
     2161 attttttctt caaaagcttg aatagtagga ttgcttattc ttgaatatat atgtccatca
     2221 acctttaaat caaataattc agcaagttgg tctatactgc tatatttaaa agttgtactt
     2281 tgatataaag gtaatactct tggttctcca ttacctggtt tataatttcc ttgcacacat
     2341 attgtttctt tattatacat tcttgtatcc cccttagatt ttagtaaaaa aaattaaact
     2401 atttatcaga tgagagttaa aataaataaa gcctgagtat ataactcagg ctctgtataa
     2461 gcttaattat actcatctct cagctttcgc cgcaggattt agcaccaatg tatataaata
     2521 tactggttgc cgggtttcat agggccagtc cctccacctc tctggataag atttatttaa
     2581 tttttctatg ttttatatct tatatatttt tcctaatttt gtcaactatt tttttaattt
     2641 ttttctttta ataattttcc caacctctac ttgaattaca gacattatgg ctaaaacaat
     2701 cgttataagc cactctaagt tatttaaaat tgttaagtta aaagcatttc taaaaacagg
     2761 tacaaacagt attactgcta ttaacaacgc agaagttaaa aatgaaatta aaagaaatgg
     2821 atttttacca ttatttctat taaacattga atctgtattt gaacgctgac taaatgctcg
     2881 taacatctgt gaaaatgcta atacacaaaa tgccattgtt tgacctacag aatgattttc
     2941 tatattaaga ccaattttat atgctaaaat agtcattatt gccacaaata taccttgggt
     3001 aacaactctt tgtataagat ttttttcaaa caaagtggtt gatttcactg gctggtgctt
     3061 cattatattt ctgcttggtg ggtcaactcc aagagcaagg gctggaagcg tagcagttgc
     3121 aaggtttacc catagaatat gaacagcaag aagcggagta tcccaattta ataatattgc
     3181 tacaaacaac gtaagtatct ctgctatatt accaacaagc aaaaattgta taactttttg
     3241 tatgtttcta tatacacgtc taccttcttt tattgcataa gcaattgttg taaagctatc
     3301 gtccattaaa atcatgtcag aagaatcttt tgcaacatct gtaccactta tacccatagc
     3361 tataccaata tcagcagact ttaaggcaga agaatcgttt acaccatctc cagtcattgc
     3421 tggtacttct ccaattcttt ttagagattg tataattcta agtttatcaa atggggacac
     3481 acgagcaaat actgtagtat ttttaacagc ttcatccagt tcatcatcac tcaaattatc
     3541 aagttcatca ccagatatca cagtattttc ttcactccat attcctaact catgagcaat
     3601 tgttgtggca gtaactttat ggtcacctgt tatcattatg gttcttatac ctgcttcttt
     3661 acaagtactt actgcatcaa ttacctcagt tcttggtggg tcaatcatac caaccatacc
     3721 tataaatgtc aaatcatatt ctatattttc accatcttct tttggtatag atgaaatctc
     3781 acgcttagca aaaccaagta cacgcaatgc acctttagac atgttaagac ataattcgtg
     3841 tatattcttt ttatccactt cagtaatgtt tctttcacct tttgaagtta atattttagt
     3901 acataggtca agaatttcgt ctactgctcc tttagtataa gcaataatct tttcatctat
     3961 attatgaaca gttgtcatgc attttctatc agaatcaaaa ggctgttcaa atacacgtgg
     4021 atattcttct tcaagagctt cttgatctat tccaaatttt ttggccataa aaattaacgc
     4081 gccctctgtt gggtcaccta atatttcatc agttttatct ggattaatac atgcatcatt
     4141 acaaagtgca ccagcataaa caatctcttt atacactaat ggatgttttt cagatgcagt
     4201 atctattgat gtcacttttc cagattcaaa atctccattg acagcaatat gcgtaacagt
     4261 cattttattt aaggttaatg taccagtttt atcacaacat attactgtag ctcctcctaa
     4321 tgtttcaaca gcaggtaatt ttctaattaa agcatttctt ttagccatac gctgtacgcc
     4381 taaagccatt acaatagtag ctgttgcagg taatccctct ggaataattg atatggcaag
     4441 ggaaattgca atcataaaca ttggcaagag aggtctctga taaaatgcac ctattgcaaa
     4501 tataacaacg catacaataa tacctatgac acttaatgtc tttccaacag tattaagttt
     4561 acgttttata ggagtatcaa aatcatcttg tgaatctaaa agatatgcta tgttacccac
     4621 ttctgtatcc ataccagttg ctactacaac acctacacct ctaccatagg ttactattga
     4681 agatgtatac gccatattgc tacggtctcc taatacacat tctttaggta agattgtatc
     4741 tgcatctttt tctgatggaa cagattctcc tgttaaagaa gcctcttgaa tttttaaact
     4801 cgatgtttct attaaacgaa ggtctgctgg aaccatcgct ccatcttcta aaaaaacaat
     4861 atctcctact acaagattac ttgctggaat aacattttct tcaccatcac gcataacacg
     4921 agctgtaggt gagttcatat tttttaatgc ttctaatgat gattctgctt ttttctcttg
     4981 aacaattcca ataactgcat ttactgttac aattattagt attacaaaag cttctgtaaa
     5041 ttcgccaaat atcaaagata atattgctgc tcctattagt atcataacca tagcatctgt
     5101 aatttgactc caaagcattt gtaaagttgt tctaggtggt ttttttctaa gttcatttaa
     5161 accattctct ttaagccgtt tttccgcctc ggtatcggtg agcccctcct ctgatgaatc
     5221 tagactttct aaaatttcat gcgttgctct tgaatgccaa ggcatctgtt tgtgattttc
     5281 cattttggaa gaccactcct ttcttttttt gatttaagaa taattatatt ttaaaatcaa
     5341 gttcaattca atacacacta agtatatttt acctaaattt aacaatgtca tttaatattt
     5401 aacaaaactt ctactctttt acaaatatgt attcactatt gttagataag ttttcttgat
     5461 acttataccc ttctaaatta aactttttca gttcttcaat atctcttatc ctatttttta
     5521 caatgtattt tgccatcata cctctagctt tctttgcttg tgtagattct actttaaata
     5581 taccattttt atttacttta aaagtacatg taatatatgt atcactatct ttaataaatt
     5641 tttctataga cttactgtat tcatttgatg aaagattgac gatagctgtt ttttctttga
     5701 ttagttcttt atatatacga tttccccaat attcgtataa attcttaaac tcacctactc
     5761 gtaattttgt ctgcatttct aatctatatt catatatact atcatatggt tttacaaccc
     5821 catacaatcc acttatgatt cgaatacagc tatttgcaaa ctcttcatct tgccttgtaa
     5881 aattttctgc ttctatattt ttatattgta ttccatcata agttaagatt gcagggcttc
     5941 ctaatctatc gaacttaatg ctttcaaatc tatttttatt taatacagct aatttttcat
     6001 taattttcat tttattttta aaatcttcta caccaaatgt ctttatattt tccactattt
     6061 cttttgtgtt atctataaaa caaggcaaac tcaaatctct atcaaatact tctatattct
     6121 tcatattttt agctggtgat aacattataa tcataaattc ttattactcc tgtttttcca
     6181 aatattcaag aatctcttgt gcatttgtgt ctggttttgc tttttcaaag actttttcaa
     6241 taattccatt ttcatctatg atgtatgtcg cacgtacaac tcccaatgca gtttttccat
     6301 acattttttt ctctttccat acatcaaaag cttttatagc aactaagtca gggtcagaaa
     6361 gtaaaataaa aggaagttca tgcttttctg caaatttttg atgtgatttt atactatctt
     6421 tacttatccc aataacttgt acatcttcct ttttaaatcc atcatatgca tttctaaaag
     6481 cacaagcttg acgtgtacat cctggtgtat tatctttagg ataaaaatat actactactt
     6541 ttttaccttt aaaatctgaa atacttacct tatttccatc tttatcttct agtgtaaatt
     6601 ctggtgcttt tgttcctata ctcaacataa atatctctcc ttttgttttt attaataatt
     6661 tt
//
LOCUS       DAEPXF010000190         6618 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain HB27
            SAMN13376389-rid12479423.denovo.197, whole genome shotgun sequence.
ACCESSION   DAEPXF010000190 DAEPXF010000000
VERSION     DAEPXF010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13376389
            Sequence Read Archive: SRR10518432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6618)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6618)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001069624.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001069624.1
            Genome Coverage       :: 315x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/15/2021 01:54:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,165
            CDSs (total)                      :: 4,076
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 89
            rRNAs                             :: 10, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 10 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 19 of 69
            Pseudo Genes (incomplete)         :: 44 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 10 of 69
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6618
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13376389-rid12479423.denovo.197"
                     /strain="HB27"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="China: Hebei"
                     /collection_date="2017"
     gene            336..494
                     /locus_tag="KRL67_003298"
     CDS             336..494
                     /locus_tag="KRL67_003298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH1524141.1"
                     /translation="MKIFSFLLFVVGICFCIAGFIGHYPAFAGATLSFFATTMISVSQ
                     VKTKKKMK"
     gene            complement(781..1485)
                     /locus_tag="KRL67_003299"
     CDS             complement(781..1485)
                     /locus_tag="KRL67_003299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBH1524142.1"
                     /translation="MEIKPLVLIVEDDKPICKFIKVSLETQNYRCVETDNGGTAISLI
                     HSLDPDLIILDLGLPDIDGIEVIGRVRACAKTNKIIVVSAREHERDKVEALDGGADDY
                     LTKPFSVTELLARVRVALRNKTQQGNINNDVPKSFEVKNLKVDYENHIVSINDEEIHL
                     TPIEYKIIELMSKYSGRVLTHKFIIDKVWGNYYESENQSLRVFMASIRRKIEKNPAQP
                     EYILTEVGVGYRMADE"
     gene            complement(1476..3083)
                     /locus_tag="KRL67_003300"
     CDS             complement(1476..3083)
                     /locus_tag="KRL67_003300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4118 domain-containing protein"
                     /protein_id="HBH1524143.1"
                     /translation="MKKERLQHEDLKEYDYDEDGYLSKKIIIKDFIKTILVMTIATGI
                     SVVFEKVGFNESNIILVYILGVLFATSITYGYFFGILSSVIGVLSFNFFFTHPLYTFL
                     AYRKDYPMTFCIMLMAAIITSTLTSRIKREVRLSHKREKMIRLLYENNKTLLKAKNKN
                     QIIEFCGSNLVNILNRTVIVTIADENNNLGKPSIYICNNDGSENIFHSVLEKEAIKEA
                     FRTGNPVGIGTKYCKNSSAYYFPIKGQESNVLGVIGVACFKIDIITENEKILLEAVST
                     QVALAIDRENLFEKNKKANLEAERERLRGNLLRSISHDLRTPLTSILGSSSTILENDD
                     VIDKETRVELLKNIYEDTSWLTHSVENILSMTRIDEGKLDIEKRPEVVDEIIAESILR
                     VKKFANSRDIKTNIPDEIIIVHVDVLLIEQVLVNLIDNAIRHTPKNSKIELTVKKESN
                     QVLFEVADNGKGIPNEDIGNIFNRFYTKNKSRNLERRGIGLGLEICKSIVEAHGGEIV
                     AFNNPSGGATFRFSIPMYYEEGKDKWK"
     gene            complement(3337..4515)
                     /locus_tag="KRL67_003301"
     CDS             complement(3337..4515)
                     /locus_tag="KRL67_003301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH1524144.1"
                     /translation="MEKLSDKRRVKAVSMETFVFIVLLAVGFGYVGSIMGAGMMFKVI
                     MSTAHALLLDTVFLIMAMAVLAGALSALLSEFGVISLVNKIFKGLMRPIWGLPGASIA
                     GVVATYLSDNPAIIPFAKDKTFTQYFKKYQVPALCNLGTAFGMGLIVTTFMIAQGKEY
                     VLPAIIGNVGAIIGSIISVRIMLTFTKKYYNYDPKNDTEKQINDKGAKLEEFREIRDG
                     NVFQRTLDAILEGGKLGVEMGMAIIPGVLVVCTLVMLLTFGPSTDPATGQAVYTGAAY
                     EGIKLLPAIGDKISFIIEPLFGFTSPEAIAFPVTALGAVGAAISLVPEFIKSGAITPN
                     DIAVFTAMGMCWSGYLSTHIGMMDALDARPLAGKAILSHTIGGLCAGICAHFIFMLVG
                     "
     gene            complement(4741..5235)
                     /locus_tag="KRL67_003302"
     CDS             complement(4741..5235)
                     /locus_tag="KRL67_003302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HBH1524145.1"
                     /translation="MHLFIEVIAAFFTALSFGVLFNMKGKNLILAGIGGSIAWFSYKF
                     LLNMGVTENLCFFIATVCFAIYCELCARIYMTPATTLSVCCLIILVPGYGIYNTMYSV
                     LTNNYIKAVEYGVSTLSCASSIALGLVFITTVFRKVNLYGVLTKIKENEKYKKSINKI
                     KQQK"
     gene            complement(5236..5997)
                     /locus_tag="KRL67_003303"
     CDS             complement(5236..5997)
                     /locus_tag="KRL67_003303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HBH1524146.1"
                     /translation="MDIDRILNFSSNAGKAMLQSGGETYRVEETIARICQSFGIDHVD
                     VFATPTAVMASVFVDGKLHSAIKRISSRRVDLNLVHEVNSLSRAISINNLDIELCEKI
                     LDEISEDNYYSDLITIFFAGIAAATFSILFGGNVEEFIAAFTVGILTKFVIMYLSKSS
                     LNDFFVNAIGAVIIASCSILILKLGFIKTLDHLIAGAIMLLVPGLALTNALRDLLDGQ
                     LISGLAKLAEVFFIGVSIAVGMFLVLGLYFKLGGI"
BASE COUNT         2249 a         1212 c          702 g         2455 t
ORIGIN      
        1 aaggcttaca taaaaagata aaaagggcaa tttttttata tcaaaaaaaa tattaaattg
       61 atattatcca tatataaact tatattatct gtataaatgt tatatttaac aattctactt
      121 gcttccctta cacttattag catatagttg taagagcttg ttacaaaaca acacttagca
      181 cactttaaaa agtgtggtct gataagtagt caacaaaaaa gacataataa agtataaaat
      241 atgatatacc tttccatgtc accaaaactg atgattttac tattaatata tgctaaaata
      301 tgcttaataa attaaatttt aatgggagaa tagttatgaa aatatttagt ttcttgttgt
      361 ttgtagtagg aatatgtttt tgtattgctg gatttatagg acattaccca gcatttgctg
      421 gtgcaacatt gagttttttt gcaactacaa tgatatctgt aagccaagtc aaaactaaga
      481 aaaaaatgaa ataggcaaat cctaacttta caagttaata ttacacaagt aattagtcta
      541 aaattggata ctttttctat tacagaaata catcatccac tttgtattct cttgaaaaaa
      601 taaaatagct atgtaaattt aagattagat agactaaatc tctatccatt tcattgtaaa
      661 atatacatag ctatttgtat ttgatttaaa atacttagat tttcttacat aacaaataat
      721 ttctatctga ttattactta tattcattta acaaccactc atcaactact catttagtat
      781 ctattcgtcc gccatacggt aacctacccc aacctcagtc aaaatatatt ctggctgagc
      841 aggattcttt tcaattttcc ttcttatact tgccataaaa actctaagcg actgattttc
      901 actttcatag taatttcccc aaactttatc tattataaac ttatgggtta aaactcttcc
      961 tgaatactta gacatcaact caataatctt atattctatt ggagttaaat gtatttcttc
     1021 atcattaatt gacacaatat gattttcata atctaccttt agatttttaa cttcaaaaga
     1081 ctttggaaca tcattattta tattaccctg ttgagtttta tttctaagag ctacacgtac
     1141 tctagccaaa agctctgtta cactaaaagg tttagttaaa taatcgtctg ctcctccatc
     1201 taaagcttcc accttatccc tctcatgctc ccttgctgaa acaacaatta ttttattagt
     1261 ctttgcacat gctctaactc gtcctattac ctcaatccca tcaatatctg gtagtcctaa
     1321 atccaaaatt atcaaatctg ggtcaagaga atgtataagt gatatagctg ttcctccatt
     1381 atccgtctct acacacctat aattttgagt ttcaagagat acttttataa atttacaaat
     1441 tggcttatcg tcttctacaa ttagaactaa tggctttatt tccacttgtc tttcccctcc
     1501 tcataataca taggtatgct aaatctaaat gtagctcctc cagaaggatt attaaaggcg
     1561 acaatttctc caccatgagc ttctactata gatttacata tctccaatcc aagaccaata
     1621 cctcttcttt caagatttct agacttattc ttagtataaa acctattaaa aatattcccg
     1681 atatcttcat ttgggattcc ttttccatta tctgcaactt caaatagcac ttgatttgac
     1741 tcttttttta cagttagttc tatcttagaa tttttaggtg tatgccttat agcattatca
     1801 attagattta ctaaaacttg ttctattaat aatacatcca catgaacgat tattatctca
     1861 tctggaatat ttgtttttat gtctctacta ttggcaaact ttttgactct taatatagat
     1921 tcagctatta tttcatcaac aacctctggt cgtttttcaa tatccaattt tccttcatct
     1981 attctggtca tactaagtat attctcaaca gaatgagtta gccaacttgt atcttcatat
     2041 atatttttca ataattctac tctagtttct ttatctatca catcatcatt ttcaagtatt
     2101 gttgaagatg aacccaatat acttgtaagt ggagttctta aatcatgaga aattgaccta
     2161 agcaagtttc ctcttaatct ctctctctct gcctcaagat ttgctttttt atttttctca
     2221 aataaatttt ctctatcaat tgccaacgca acctgcgttg aaactgcttc taatagtatc
     2281 ttttcatttt ctgtaataat atctatctta aaacacgcaa cacctataac accaagaaca
     2341 tttgattctt gaccttttat agggaaataa tatgcactgc tgtttttaca atattttgtc
     2401 ccaattccaa ctgggttacc tgtcctaaaa gcttctttta tagcttcctt ttctaatact
     2461 gaatggaata tattctcact tccatcattg ttacaaatat aaatacttgg cttacctaga
     2521 ttattattct catctgcaat agtcactatt acagttctat ttaatatatt tactaaatta
     2581 gacccacaaa attcaataat ttgattttta ttttttgctt taagcaacgt tttgttattc
     2641 tcatagagta atcttatcat tttttctctc ttatgagaaa gtctcacttc tctttttatc
     2701 ctagaagtta aagtacttgt tataattgct gccatcagca taatacaaaa ggtcatagga
     2761 taatcttttc tataagctaa aaaagtatat aatggatgcg taaagaaaaa attaaatgat
     2821 agcactccta ttactgaaga aagtattcca aaaaaatatc cataggttat acttgtagca
     2881 aatagtacac ctaatatgta tactagtata atattagatt cattaaaacc taccttttca
     2941 aaaactaccg atattccagt agcaattgtc ataaccaata tagtttttat gaagtcttta
     3001 ataattatct ttttactgag atagccatct tcatcataat catactcttt taagtcttca
     3061 tgttgtagtc gttccttttt catttactac aattcctcct tattgttact gtttaaattg
     3121 gctctatttt atataataca attttatcat aataaattca atcaatacat ttaattaatg
     3181 tacttaaatt actaaaaggt gctgtcttaa aatgaaaata aatctctatt tcaaaacagc
     3241 acctatatat ttaaattact aatttagttt ttacatactt aaatcaatag ttttattcaa
     3301 tttcataaat tataatgctt gaattatcga acattcttat ccaacaagca taaatataaa
     3361 gtgggcacat atacctgcac acaagccacc tatggtatgc gaaagtatcg ccttaccagc
     3421 caatggtctt gcatctaaag catccatcat acctatgtgt gtacttaaat atccactcca
     3481 gcacataccc attgctgtga atactgctat atcattagga gttatagcgc ctgattttat
     3541 aaactctggt actaaagata ttgctgctcc aacagctcct aatgcagtta cagggaatgc
     3601 tattgcctct ggtgatgtga atccaaataa tggttctatt atgaagctaa tcttatcacc
     3661 aatagcaggt aataatttta taccttcata tgcagctcct gtgtaaacag cttgacctgt
     3721 agcagggtct gtagatggtc caaaagttaa taacataact aatgtacaaa ctactaaaac
     3781 tcctggtatt atagccatac ccatttcaac acctaactta ccaccttcaa gtatagcatc
     3841 caatgttctt tggaatacat taccatctct tatttctcta aactcttcaa gttttgcacc
     3901 tttatcattt atttgctttt cagtatcatt tttagggtca taattataat actttttagt
     3961 aaaagtcaac ataattctaa cactaataat actacctatt attgccccaa cattacctat
     4021 tatagctggt agtacatatt ctttaccttg agctatcata aaagttgtta caataagtcc
     4081 cataccaaag gctgttccta aattacataa agcaggtact tgatacttct taaaatattg
     4141 tgtaaatgtt ttgtctttag caaatggtat aatagctgga ttatcagata aatatgttgc
     4201 aactactcct gctatactag caccaggtaa gccccatata ggtctcataa gacccttaaa
     4261 tatcttatta actaaagata ttactccaaa ctctgaaagc aacgcactaa gtgcacctgc
     4321 aagaactgcc atagccatta ttaaaaatac tgtgtctaat aaaagcgcat gtgctgtact
     4381 cattattact ttaaacatca ttcctgcacc catgatgctt cctacatatc caaatcctac
     4441 cgccaatagt acaataaata cgaaggtttc catacttact gccttgactc ttctcttatc
     4501 cgacagcttt tccatcttga aatcttcccc cttttaattt taaattaagg ttcctaacat
     4561 tggtacaaat tttttggtac aaactgagtt ctaaaactta cactcgtaat gcaagttgtc
     4621 agttttttga aataaaaaaa aattacgttt atagaataag aatatcctct ttaatttgga
     4681 tactcttatt ctattttatg ttattatatt atcatccttt tgtttaaaag tcaatttttt
     4741 ctatttttgt tgtttaattt tatttatact ctttttatat ttttcatttt cttttatctt
     4801 tgtaagcact ccatataagt tgacttttct aaatacagta gttataaata ccaatcctaa
     4861 tgcaattgaa cttgcacatg ataatgtact cacaccatat tctacagctt ttatataatt
     4921 attggtcaaa acagaataca tagtattata tattccataa cctggtacta atattattaa
     4981 acaacaaaca cttaatgtag ttgctggagt catataaatt cttgcacata actcacaata
     5041 tatcgcaaaa caaactgtag ctataaaaaa acataagttt tctgttactc ccatatttaa
     5101 aagaaattta tatgaaaacc aagctatact tccacctatt cctgctagta ttaagttttt
     5161 ccctttcata ttaaatagca ctccaaagct taaggcagta aaaaaagctg ctattacttc
     5221 tataaacaaa tgcacttata tcccccctaa cttgaagtac aatccaagta ccaagaacat
     5281 tcctactgct atagatacac ctataaaaaa tacttctgct aattttgcaa gaccagaaat
     5341 caactgtcca tctaacaaat ctcttagtgc attagtcaaa gcaagcccgg gaactaagag
     5401 cattattgct cctgctatta aatggtctaa tgtctttata aatccgagct ttaaaattaa
     5461 aattgaacaa gaagctataa taactgctcc aattgcattt acaaaaaaat cattaaggga
     5521 agatttactt aaatacataa taacaaactt agtcagaatc ccaacagtaa atgcagctat
     5581 aaattcttcc acatttcctc caaataatat agaaaaagtt gctgctgcaa tgcctgcaaa
     5641 aaatattgtt attaaatccg agtaatagtt atcttcactt atttcatcta aaattttctc
     5701 acataattca atatctaaat tatttatact tattgctctt gataatgagt taacttcatg
     5761 aaccaagttt aggtctactc ttcttgagct tattctttta attgctgaat gtaattttcc
     5821 atctacaaat acagatgcca ttacagctgt tggagttgca aatacatcta catggtctat
     5881 tccaaaagat tgacatattc tagctatcgt ttcttcaact ctataggttt ctcctccact
     5941 ttgcaacata gctttacctg catttgagga aaagtttaaa attctatcta tatccacaaa
     6001 acatatctcc cattctttta tagtattatt catattttaa tacaagatat tatttattta
     6061 aactatttta aataattcat atccaatatt tacattttat gaaaaaactt gttttttatg
     6121 cctgtcaaaa ccaaatttta aggttgttta caccataaaa tggctgaatt aatatttgac
     6181 aaacactttc aaccatttat ataaccttct aaattatgtg attttttact acaaataaaa
     6241 ttatatctct tttaattctt tctcttgagt atttaagttt acaacaactt catatttata
     6301 tatgtacaat ataaaacatt atttttatga tacaaaatat aatttgtaaa tgtatatatg
     6361 tattaactgt gtatctataa attattctta actttatcat ttttttcata tatcttcatc
     6421 tatatcgacc atatattaat tacgttgacc atatattaat aaatgatatt acatagactg
     6481 aagtatatta aaaaacttaa atttagttat ttaacaacat aatactctaa tttaatctat
     6541 aaaaatccat atctaatata atgcaactct tattttaaca ccatctatta cacaaaacaa
     6601 catattaaaa agtaaaat
//
LOCUS       DAEPXF010000191         6577 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain HB27
            SAMN13376389-rid12479423.denovo.198, whole genome shotgun sequence.
ACCESSION   DAEPXF010000191 DAEPXF010000000
VERSION     DAEPXF010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13376389
            Sequence Read Archive: SRR10518432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6577)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6577)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001069624.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001069624.1
            Genome Coverage       :: 315x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/15/2021 01:54:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,165
            CDSs (total)                      :: 4,076
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 89
            rRNAs                             :: 10, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 10 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 19 of 69
            Pseudo Genes (incomplete)         :: 44 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 10 of 69
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6577
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13376389-rid12479423.denovo.198"
                     /strain="HB27"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="China: Hebei"
                     /collection_date="2017"
     gene            901..2256
                     /locus_tag="KRL67_003304"
     CDS             901..2256
                     /locus_tag="KRL67_003304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HBH1524147.1"
                     /translation="MSSTVKKKGKFPIGFYICSTTFSFERAAYYSSKYLIYIFLTTAI
                     IHGGLGIEKGQAAIMQANLVAFTYLAPIIGGYISDRWIGARYTIPIGMLIMGVGYYLG
                     SIATTVSMVNAMIILVSIGTAFFKGNVSAVNGQLFDNQEELDTAFSVQYSFVNIGSFI
                     GTTAVGILYLKTFAKNGVLGFSQCFFIAAVLCVIGAIWFTYGWRFLGNAGKRPFKEGV
                     VAEKVEEKDKSPLTSMDKKRIWAIILISFFSVIFWVFWYLTYLAVYDYGAAFVNMTVG
                     GFEVPLAWFDSLNALVCIVLGPVLGALWFKLASRPQGDMSLFKKTGLGLIFLGLAFLM
                     LVGAEFSRGVGAPETAKASIIWIIMFGILLSFGEMLFSPLGNSFVSKYAPKKLLGVLM
                     GVWTFATFIAGQGYGYIYAFTLKFEMIKVYTIIPVILFVSAILLFLFDKTLSRLVEDD
                     K"
     gene            complement(2483..3364)
                     /locus_tag="KRL67_003305"
     CDS             complement(2483..3364)
                     /locus_tag="KRL67_003305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HBH1524148.1"
                     /translation="MNKIKIKQVDAFTTIPFGGNPAGVVTDASNISDEIKQNVAKEMN
                     LSETAFVSESNVADFKVQFFTPGLEVDLCGHATIGTFHALLEEGKLNTNKSIFYQETK
                     AGVLSVELKEINNENIFMMEQNTPEFENLDNYRKELAQMIGLHEDRLLEYPIMKVNTG
                     LWWLVFGLKSLDDLKNISPDLNKIKKFSGDNNIIGITPFCIETIDKKCDYHLRSIAPY
                     VGVTEDPVCGTGNACVSSYIVHHNILDKTSFIGEQGNFINRPGKVYVNITKENNNITS
                     VKIGGSAYTVLSGEIMY"
     gene            complement(3357..4103)
                     /locus_tag="KRL67_003306"
     CDS             complement(3357..4103)
                     /locus_tag="KRL67_003306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrmB family transcriptional regulator"
                     /protein_id="HBH1524149.1"
                     /translation="MDNIINELQNIGFTKYESQIYISLLKESPLTGYEISKLSGVPQS
                     KVYENITRLLNNNIIINVGTDPIKYIPINPNELLKNKKKEFDNSMDNLKNSLQILNKN
                     KSVEYVLNIKGDKNILKKALEMIKNAKQEIIISCPYEEILELKKELLIAYKKNINIEI
                     LLLENKSLEGLDNIHIHGGNKTTLENKMLYKGIILIVDNDEILTGNLSEGSEAISIWT
                     KNSNILYIGVQYIKHEIYISEQIKGGYKNE"
     gene            4369..4686
                     /gene="trxA"
                     /locus_tag="KRL67_003307"
     CDS             4369..4686
                     /gene="trxA"
                     /locus_tag="KRL67_003307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HBH1524150.1"
                     /translation="MVKIINNNEFINEVENKDGLVVVDFFATWCGPCKMLSPIYEALG
                     NEMVEKANFLKVDIDQSIELAQKFEVSTVPTMLIFKDGKPVDRLIGFMPKENLKNKIE
                     SYM"
     gene            complement(4892..6166)
                     /locus_tag="KRL67_003308"
     CDS             complement(4892..6166)
                     /locus_tag="KRL67_003308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HBH1524151.1"
                     /translation="MQFMDVSKYIEGANLKVKTKNGETVKRIYFNNSATPLVLKNVVD
                     NLNCDIPWLTYINAPGIISEKNTLKYENVRSTILKLIDGDEEKDSVIYVKNATEGINF
                     LAKFFKDENPDKFVITTAMEHMANYLPFKVNFPTKVVGITEKGELDLCQLENILKNYA
                     GNVSLVTVTGASNVTGITTPIYEIARMAHKYGAKILVDIVQVIQHRPFSMMPHECDEH
                     IDFVVFSAHKCYSPLNGGALVGSKKFFEKFNPVLYGSGSTKFVNDNKILYANVPQRFE
                     AGYPEYFGTLAMGKALNTLNKIGLNRISKYERELFLYAKEEMSKIPNIIIYGDKSNNV
                     IIPNISFNISNMYYKDVAKYLVDNFGIETGAGVVGADIYVQKLLGISPKEAYIRFMRE
                     NPVGLVRISLGMYNTFDEVDSFIYAIKTLAFK"
BASE COUNT         2281 a          834 c          950 g         2512 t
ORIGIN      
        1 tatatttgag taaataagta aagtaataaa aaaatcaaga tttcatatct aaaaaactat
       61 tctgaacatt aatttaactt atattttcat aggaaatatt ttatataatg tacttgacaa
      121 atataattta aatttttcta attatttact gtcgattaat agattttttt cttgcatata
      181 agtatgtatt tatggtacga tttacatggt gaaataaaaa aggggaggtg aaaaataagt
      241 attcgtgaaa aagttttcag tttgaggtat tttataaata atatattata aataaagagt
      301 ataacctcaa taaatagtac caaagcgaat atatgggtga ttactttaaa ataacagttt
      361 ttatgtttag attcacagtg tttattataa actaatgtgt aagaaataag tctaatctgt
      421 gaatagatag tagtttattt atttaagttg agaattggtt gggtgaagta atatataaat
      481 ttaaaaagta aataaaaaca ttatttgaac aaatatatgt tgctttattc gagactcctt
      541 taaaaaagca ctaaaatgta attacagaag ttgctacaaa aggggaattt tagtttgtaa
      601 aatactatca cgtaaaaaat aagaattgca tatggcgaag gttttttcat ggtattagat
      661 gagaatatac tgatttataa atatatctga aaatagtgaa tttgtataat ttaaatttat
      721 gactaagaag tgtattaatt ttattccatg aaaatatgta ctctacaaat aaatgcgcaa
      781 ttatcttaat aataattaca aattgtttta caaaataata tcataatatc tttcataaaa
      841 agaaagaatt tggacaaaac gtaaaaaaat aaaaatacaa ttaagaaagg atttaaaact
      901 atgagctcaa cagtaaaaaa gaagggtaaa tttcctatag gattttatat ctgctcaaca
      961 actttctcat ttgaaagagc agcatattat tcttcaaagt acttaatcta tatattcctt
     1021 acaacagcta taattcatgg tggtcttgga atagaaaagg gacaagctgc tataatgcaa
     1081 gctaatcttg ttgcattcac atatcttgca cctataattg ggggatatat atcagataga
     1141 tggataggag ctagatatac tatacctata ggtatgctta tcatgggggt aggatattat
     1201 cttggttcta ttgcaactac agtttctatg gtaaatgcaa tgataatatt agtttcaatt
     1261 ggtactgcat tctttaaagg gaatgtatct gcagtaaatg gacaactctt tgataatcaa
     1321 gaagaacttg atacagcatt ttcagtacag tattcttttg ttaacatagg ttcttttatt
     1381 ggaactacag cagttggtat tctttacctt aaaacatttg caaaaaatgg tgtacttgga
     1441 ttttctcaat gtttctttat agcagctgta ctttgtgtaa taggtgcaat atggttcaca
     1501 tatggatgga gattccttgg aaatgcagga aagagacctt ttaaagaagg cgtagttgct
     1561 gaaaaagttg aagaaaaaga taagtctcct cttacaagta tggataagaa gagaatttgg
     1621 gctattatac ttatatcatt cttctctgta attttctggg tattctggta tctaacttat
     1681 cttgcagttt atgattatgg tgcagcattt gtaaacatga ctgttggagg atttgaagtt
     1741 cctcttgctt ggtttgactc attaaatgca cttgtatgta tagtattagg accagttctt
     1801 ggagcattat ggtttaaact tgctagtaga ccacaaggag atatgagttt atttaaaaag
     1861 acaggtcttg gattaatatt cttaggtctt gcattcttaa tgcttgttgg tgcagaattt
     1921 tcaagagggg ttggagctcc tgaaactgct aaagctagta taatatggat aatcatgttt
     1981 ggtattcttt taagttttgg tgaaatgtta ttctcaccac ttggtaactc tttcgtaagt
     2041 aagtatgcac ctaagaagct tctaggtgtt cttatgggag tatggacatt tgctactttt
     2101 atagctggtc aaggatacgg atatatatat gcatttactc ttaaatttga gatgattaaa
     2161 gtgtatacta taattccagt gatactattt gtttcagcta tattattatt cttatttgac
     2221 aagacattaa gtagacttgt tgaggatgat aaataatcag taagttaatt attgtgtttt
     2281 atagattaat ctattgtaat tatgttgtat tagattgatt tataactaat taataataag
     2341 aataaaattt tacttctaag tacaatgtta tgatatatat gattatgata tgtattagag
     2401 tatgattatt aaataaaaat aacaaataaa aatactagcc ttagttagct agtattttta
     2461 tttgttttta ttaggtaggg gattaataca taatttcacc acttaaaaca gtataagcac
     2521 ttcctccaat ttttacagaa gttatgttat tgttttcttt agttatattt acatatactt
     2581 ttccaggtct gtttataaag ttaccttgct caccaataaa actagtttta tctaaaatat
     2641 tgtgatgtac tatataagat gatacgcaag cgttcccagt tccacataca gggtcttctg
     2701 taactccaac atatggtgct atagatctta aatggtagtc acacttttta tctatagttt
     2761 ctatacaaaa tggagttatg ccaattatat tattatcacc actaaatttt ttaattttat
     2821 ttaaatctgg agagatattt tttaaatcat ctaaagattt tagaccaaat acaagccacc
     2881 aaagtccagt atttaccttc ataataggat attcaagaag tctatcttca tgcaaaccta
     2941 tcatttgtgc aagttctttt ctgtaattat ctaaattttc aaattcaggt gtattctgtt
     3001 ccatcataaa tatattttca ttatttattt ccttaagctc tacagataat acaccagctt
     3061 ttgtttcttg ataaaatata ctcttattag tattaagctt tccttcttcc aaaagtgcat
     3121 ggaaagtacc tattgttgca tgaccgcata aatcaacttc taaacctggt gtgaaaaatt
     3181 gtaccttaaa gtctgcaaca tttgactctg aaacaaatgc agtttctgat aaattcattt
     3241 ccttagccac attctgctta atttcatcag aaatattaga ggcatcagta acgacaccag
     3301 caggatttcc accaaaaggt atagttgtaa aagcatcaac ttgtttaatc ttaattttat
     3361 tcatttttat aacctccttt tatttgttct gaaatataaa tttcatgttt tatgtattga
     3421 actccaatat ataaaatgtt agagtttttt gtccaaattg aaatggcttc actgccttca
     3481 gataagtttc ctgtcaatat ttcatcatta tctacaatca aaataattcc tttgtaaagc
     3541 attttatttt caagtgtagt tttattacct ccatgaatat gaatattatc aagaccttct
     3601 agacttttgt tttctaaaag tagtatttca atattaatat tttttttgta agcaattagg
     3661 agttcttttt ttaattcaag aatttcttca taagggcaag aaattataat ttcttgtttt
     3721 gcatttttta tcatttctaa tgcttttttt aatatatttt tatctccttt tatgtttaaa
     3781 acgtattcta cacttttatt tttatttaat atctgtaagc tattctttag attatccata
     3841 gaattatcaa attctttttt cttatttttt aataattcat tagggtttat gggtatgtac
     3901 ttaatagggt cagttccaac attgataatt atattattgt ttaatagcct tgttatattt
     3961 tcatagactt ttgattgtgg tacacctgat aatttactta tttcatatcc tgttaaaggg
     4021 ctttccttta ataatgatat gtatatttga gattcgtatt tagtaaatcc tatattttgt
     4081 aattcattaa ttatattatc catagagaaa ctcctttgat gaaagattag taactaccta
     4141 tgtagttatt aatttaattt tataatctaa gggaaaaaat gtcaattata ttaagaaaaa
     4201 ataaaatata ttagaaatat ttagtctcat aaatatttaa tcttataaat ctatttatca
     4261 taatattaaa tctattaaaa ataggcggtt acaaaaaagt gcctaattga actgttatat
     4321 aggtcgtagt atattaatat caagaagaca ataaaggaga tgtatattat ggtaaaaata
     4381 ataaataata atgaatttat aaatgaagta gaaaataaag atggtttagt agttgtagat
     4441 ttctttgcga cttggtgtgg accttgtaag atgctatcac caatatatga agctttagga
     4501 aatgagatgg ttgaaaaagc aaatttcttg aaggtggata tagaccaaag tatagaatta
     4561 gctcagaaat ttgaagtatc tacagtacca actatgctaa tatttaagga tggaaaacct
     4621 gtagatagat taataggttt tatgcctaag gagaatttaa aaaataaaat tgaatcatat
     4681 atgtaattaa attaaattgc agtaattaaa ataaatctca tatataaaca agaaactcat
     4741 tgtaatgagt tttttgttta tacagtgtaa aatagtttgt atagttacaa atgagtgcct
     4801 aattgctaat gagattgact attgatataa tttgcatttt tgtgaaaaca aaactattct
     4861 ctatttattg ctaaaaaaga atagaaaaat cttatttaaa agccaaagtt ttaatagcat
     4921 atataaagct atcaacctca tcaaaagtat tgtacattcc taagcttatc ctaacaagac
     4981 ctacaggatt ttccctcata aatctgatgt atgcctcttt tggacttata cctaacaatt
     5041 tttgtacata aatatctgca cctactactc cagctccagt ttctattcca aagttatcaa
     5101 ctaaatattt tgctacatct ttgtaataca tattacttat attaaaagat atatttggga
     5161 taattacatt atttgattta tctccatata ttattatatt aggtatttta ctcatttctt
     5221 ctttagcata taaaaataat tctctttcgt attttgaaat tctgtttaga ccaattttat
     5281 ttaaagtgtt cagtgctttt cccattgcaa gagttccaaa atactctgga tatcctgctt
     5341 caaaacgttg gggcacgttt gcatatagta ttttgttatc atttacaaat ttggtagaac
     5401 ctgagccata tagtacagga ttaaattttt caaagaattt tttagatcct accagagctc
     5461 ctccatttag tggagaataa catttatgag cactaaaaac aacaaaatct atatgctcat
     5521 cacattcatg tggcatcatt gaaaaaggtc tatgctgaat tacttgcaca atatccacca
     5581 atatctttgc accatactta tgagccattc ttgctatttc atatatagga gtggttatac
     5641 ctgtaacatt actagctcct gtaactgtaa cgagagatac attaccagca taatttttaa
     5701 gtatattctc taattgacat aaatcaagtt ctcctttttc tgtaattcca acaacttttg
     5761 taggaaaatt aactttaaaa ggcaggtaat tagccatatg ctccattgct gtggtaataa
     5821 caaatttatc tggattttca tctttaaaaa attttgctaa gaagtttatt ccttctgtgg
     5881 catttttgac ataaataaca gaatcctttt cttcgtctcc atcaataagt ttgaggatag
     5941 tagagcgtac attttcatat tttaaagtat ttttttctga gattattcct ggagcattaa
     6001 tataagtaag ccaaggaata tcacagttta agttatccac tacgtttttt aaaactaatg
     6061 gagtggcaga gttgttaaaa tatattcttt taacagtttc accattttta gttttaactt
     6121 taaggtttgc accttcaatg tatttagata catccatgaa ttgcataaaa aaaacccctt
     6181 atctaataaa ctattctgtt ttttattaaa aaagctcaag ttttaatcaa gagcttgttt
     6241 aatatatgtt atgtttttat aaggggtttt gttaattaat tcttgttgag atgtttatta
     6301 aattacacga aaatgagcta cagttggggg aaactgtagc tcacttgatt ttgggggaga
     6361 taggttgaac ttattgttta tccgtatcat atttttagta tagtcactta ttttaattta
     6421 tatacatttt tttataaatt ttacataaaa tgttaaacgc taattagaaa taattatgac
     6481 tatgactatt aaagttgcaa aaatagtaaa gaagttgatt agaacataat ggaaaatata
     6541 ttgtatataa ttagaaaaaa agattttata tgttata
//
LOCUS       DAEPXF010000192         6508 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain HB27
            SAMN13376389-rid12479423.denovo.199, whole genome shotgun sequence.
ACCESSION   DAEPXF010000192 DAEPXF010000000
VERSION     DAEPXF010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13376389
            Sequence Read Archive: SRR10518432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6508)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6508)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001069624.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001069624.1
            Genome Coverage       :: 315x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/15/2021 01:54:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,165
            CDSs (total)                      :: 4,076
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 89
            rRNAs                             :: 10, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 10 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 19 of 69
            Pseudo Genes (incomplete)         :: 44 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 10 of 69
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6508
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13376389-rid12479423.denovo.199"
                     /strain="HB27"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="China: Hebei"
                     /collection_date="2017"
     misc_binding    98..309
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            472..1761
                     /locus_tag="KRL67_003309"
     CDS             472..1761
                     /locus_tag="KRL67_003309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytosine permease"
                     /protein_id="HBH1524152.1"
                     /translation="MEEEKINYALEQIPENQKRGWVAMFSVLVAIGVDLSSVILGAEL
                     AQSMPMKQAILSVIVGSFFSAILYTTCSLVGSSTSLSTSMITKYVFGEAGAKIFSLVI
                     GVSLLGWFGVQVGFFAQNAQIIIKDIFNLDVSMQILSLIGGLLMMSTAIYGYKAMEKL
                     SVYSVPFLLVLMMLTIFLAFRANGISVDDNMKSTMTFAGGVSLSMSIIIVGAIVSPDI
                     SRWAKSRRDCALSSFLGIQFGNAFMIIVSIVLVKCMGTSDIMRIFITLGIAIPGIIVL
                     TLAQWTTNTSNVYSASLSIALVLKKAPEKVLTIVLGIIATLLAVFGIYEGFIGFLNLL
                     GIVIAPVGGVYTAEYYIVKQELKGFDKGVLYKPIVKRSIVSWIIGILITYLSTYGFIT
                     LTTIAPLDGFIAGFVVQSIIGKVLCSTKNKQEIDKAV"
     gene            1815..2894
                     /locus_tag="KRL67_003310"
     CDS             1815..2894
                     /locus_tag="KRL67_003310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF917 domain-containing protein"
                     /protein_id="HBH1524153.1"
                     /translation="MRFLTEESVDKIAVGAAVLGTGGGGDPYVGKLVAKQAIKKYGPV
                     KVISLDELDDDALVVPVSGMGSPVITIEKLLSEVELTTPLEIMEKLLNRKVDVIIPIE
                     IGGINSLMPIAVAAKKGLPILDADSMGRAFPEAQMVTFYLEGYEASPAVMSDEKGNSA
                     ILYPVDGIWSERLARTLTVEMGGSSSISDYNLSGAQVKKAAIGNTLTIAETIGGFLLE
                     SKSDSKGAVENILKELRGYKLFEGKVIDIKRELKGGFTRGHAFFAGINEYDGEYSILF
                     QNENLIAKKGNTPLCITPDLIAVLDLETGFPITTERIKYGSRVMVVAFPCNEKWRTEK
                     GIETVGPGYFGYDVEYKTVEELQGK"
     gene            2915..4459
                     /locus_tag="KRL67_003311"
     CDS             2915..4459
                     /locus_tag="KRL67_003311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020246462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HBH1524154.1"
                     /translation="MYRVGIDVGGTNTDACILDGELKVIHSVKVATTKDVETGVYNAL
                     KKVIDESKIDHSLIKYAMLGTTHCTNAIVERKRLNNVAMIRIGKPATTAILPFIDWPE
                     DLREKIELDSILVSGGYEFDGRKINELCKDEVVEFCNRIKDKVESVAISGVFAPVNKQ
                     QEEEVAKWVREILGDIPISLSNEIGNIGILERENGAILNSALSQVGKAVSLGFEKALR
                     DLGIDAQIYFSQNDGTLMNLEYTMKYPIFTIGCGITNSIRGASFLSKVKNAIVLDVGG
                     TTSDLGVLYNGFPRESSIPVTIGGVHTNFRMPDVLTIGLAGGTCVKGEKDNIKIGPES
                     VGYRITEDAIIFGGDTLTLSDVVTKLDMAFEDRKSNVENLDKEYCDEVYKKVILKLED
                     AVDQMKTSQDDVELVLTGGGSIIVPEKLKGISNVIRPPHYTAANAIGAALGQISGDVE
                     KVYSLDKMSREDAIEDAKKEAVNKALKAGAKEDTIEVLNIEDVPLAYLPGNALLIKVK
                     VVGDLI"
     gene            complement(4636..5178)
                     /locus_tag="KRL67_003312"
     CDS             complement(4636..5178)
                     /locus_tag="KRL67_003312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1697 domain-containing protein"
                     /protein_id="HBH1524155.1"
                     /translation="MKSYIALLRGINISGKNKIIMSELKASFVELGYLEVSTYLNSGN
                     VVFLSDVDDSKVISNTIRLMIKKQFELEIPVFIISQEELKDILNNAPNWWGDNNKEIY
                     DNLIFMLPDLSYDEFYDEVGNPKEEYEKVYHYKNAIFWSFSRKDYKKTNWWSKTASSN
                     VSDKITIRTANTVRKIVGMR"
     gene            complement(5326..5610)
                     /locus_tag="KRL67_003313"
     CDS             complement(5326..5610)
                     /locus_tag="KRL67_003313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HBH1524156.1"
                     /translation="MAKNTKQTTVHIDETLLKEVKRIGINENKSISQIINESIIDYVL
                     VYRENEDKEKNEHDKNLNEIINEFKKEEEKRMQAKSAARAIAEAKKRVLY"
     gene            complement(5673..6437)
                     /locus_tag="KRL67_003314"
     CDS             complement(5673..6437)
                     /locus_tag="KRL67_003314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HBH1524157.1"
                     /translation="MNNYLKFATYQLIGFTSSIPKLIKIKKNPDKFSLKEKFEFMQKQ
                     AKKSLDIVNIELNIIGKETLPKEPLLFVVNHSSMLDSFILTASVERPIGCVIADEPVW
                     RNIPIFKEWAKLLRCVYVNRKNNREGIKSIAEASQNILTGQSMAVFPEGDLTWIKEPN
                     SLVSEFRSGALKIAYKAKCPIVPLVIKNSKDTYEGYQPIGKINSVPVEVEFLEPIYDH
                     IENPRLKSSVLGENIKNKMINTIENFRKSNKTFKEF"
BASE COUNT         2267 a          811 c         1127 g         2303 t
ORIGIN      
        1 tataaaacta aaaaatgcaa gtaattgact ttttaagttg acaaaatatt gcatatatat
       61 tatgatataa atcataaatt aactacaaat caatattata tattcgtaga agaagaaagt
      121 agcataaagg aattttacag agagttagat ttggctgaga actaatattg gaatttatgt
      181 gaaaaggact ttggagaata ctattgtaat tatgttagaa tagtggttta attcgcctta
      241 agaaaatgag tgagtaaaat taatttttac taataagagt ggtaacacgg atttatattc
      301 gtctcttgga tttttccaag aggcgttttt tattataaaa ttatttcaga aaatttaaaa
      361 ctatatatta aatgaaaatt ataaaaatat gatagttaat aaatttataa attagaaaat
      421 tgtaaaatat actaaggtca gtagctttac gatttaaatg gagggatgaa aatggaagaa
      481 gaaaaaatta actatgctct ggaacagatt ccagagaatc aaaaacgtgg ttgggtagct
      541 atgttttcag ttttagtagc aataggtgtg gatttatcat ctgttatttt aggcgctgag
      601 ttggcacaga gtatgccaat gaaacaagca atattgtcag taattgttgg ttcatttttt
      661 tctgcaattt tgtatactac atgctctttg gttggttcat ctacaagttt atctacatct
      721 atgataacaa agtatgtatt tggagaagct ggagcaaaaa tattttctct agttataggg
      781 gtttctctat tagggtggtt tggagttcaa gtaggtttct ttgctcaaaa tgcacagata
      841 ataataaaag atatatttaa tttagatgtc agtatgcaga ttttaagcct aataggtggc
      901 ctcttgatga tgagtactgc aatttatgga tataaggcaa tggagaagtt gagtgtgtat
      961 tcagtaccat ttttattagt tcttatgatg ctaactatat tcttagcttt tagagcaaat
     1021 ggaatttcag ttgatgataa tatgaaatct actatgacat ttgcaggtgg agtttctctg
     1081 tcaatgagta taataatagt tggtgcaatt gtatctcctg atataagtcg ttgggctaaa
     1141 agtagaagag actgtgcgct ttcaagcttt ttgggaatac aatttggaaa tgcttttatg
     1201 ataatagttt caatagtatt agtaaaatgt atgggaactt cagatattat gagaattttt
     1261 attacattag gaattgctat accagggata atagtactta cattagctca atggactaca
     1321 aatacaagta atgtatattc agcatcatta agtatagctc ttgtacttaa gaaggctcct
     1381 gagaaagtat taactatagt ccttggtatt atagcaacat tattggctgt atttggaata
     1441 tatgaaggtt ttataggatt tttaaattta cttggaattg taattgcacc tgttggtggt
     1501 gtatatacag cagaatatta catagtaaag caagaactta aaggctttga taaaggagta
     1561 ttatataaac caatagttaa acgttcaata gtgtcttgga taattggtat attaataact
     1621 tacctttcaa catatggatt tataactctt actactatag cacctttaga tggttttata
     1681 gcagggtttg tagtacaatc tattattggg aaagtgcttt gttcaacgaa aaataagcaa
     1741 gaaatagaca aagcagtata aagaaatttg tttataaagt ataaaataaa ttagaaatat
     1801 gaggagaaag taacatgaga tttttaacag aagaaagtgt agataaaata gcagttggtg
     1861 cagcagtgct tggaactgga ggtggaggtg acccatatgt aggtaaattg gttgcaaaac
     1921 aagccattaa aaagtatggt cctgtaaaag taataagttt agatgaactt gatgatgatg
     1981 ctttggtagt tccagtatct ggtatgggtt ctcctgtaat tacaatagaa aaattacttt
     2041 cagaagtaga gttaactacc cctttagaaa taatggagaa attgttaaat agaaaagtgg
     2101 atgttataat tcctatagaa ataggaggga taaactcact tatgccgata gcagttgctg
     2161 ctaaaaaagg acttcctatc ttagatgcag attctatggg tagagctttc cctgaagctc
     2221 aaatggtaac tttttatctt gaaggatacg aggcatctcc tgccgtaatg tctgatgaaa
     2281 aaggtaattc tgctatcctt tatcctgtgg atggaatttg gtcagaaaga ttagctagaa
     2341 ctttaactgt agaaatggga ggaagttctt ctattagtga ctataattta agtggagctc
     2401 aagttaaaaa agctgctata ggcaatactc ttacaatagc tgaaactata ggaggatttt
     2461 tacttgaaag taaatctgat tcaaaaggtg ctgttgagaa tatactaaaa gaattacgtg
     2521 gatacaagtt atttgaagga aaagtaattg atattaagag agagttgaaa ggtggattta
     2581 ctagaggaca tgcctttttt gctgggatta atgagtatga tggagagtat tctatattat
     2641 tccaaaatga gaacttaatt gccaaaaaag gtaatacacc actttgtatt acaccagatt
     2701 taatagcagt attagattta gaaactggat ttccaataac tacagaaaga ataaaatatg
     2761 gctctcgtgt tatggtagta gcttttcctt gtaatgaaaa gtggagaaca gaaaaaggaa
     2821 ttgagacagt tggacctgga tactttggat atgatgtgga atataaaaca gtagaagaat
     2881 tacaaggaaa ataaaagttt aggaggaaat tttgatgtat cgtgtaggaa ttgatgttgg
     2941 aggaacaaat acagatgctt gtatactgga tggggaatta aaagtaattc attcagttaa
     3001 ggtagcaact acaaaagatg ttgaaactgg tgtttacaat gccttaaaaa aagttataga
     3061 tgaaagtaaa attgatcatt cattaataaa atatgctatg cttggtacaa ctcattgtac
     3121 aaatgctatt gtagaaagaa aaagacttaa taatgtggct atgataagaa taggaaagcc
     3181 agccactact gccattcttc catttataga ttggccagaa gacttaagag aaaaaataga
     3241 attagattca atattggtat ctggtggata tgagtttgat ggtagaaaga taaatgaact
     3301 ttgtaaagat gaagttgtag agttttgcaa tagaataaaa gacaaagtgg aaagtgttgc
     3361 aataagtgga gtatttgctc cagtaaataa acaacaagaa gaagaagttg caaaatgggt
     3421 tagagaaatt ttaggggata ttcctatatc tttatcaaat gaaataggta atataggtat
     3481 attagaaaga gaaaatggag ctattttaaa ttctgcattg tctcaagtag gtaaagctgt
     3541 atctttagga tttgaaaaag cacttagaga cttaggtata gatgctcaaa tatatttcag
     3601 tcaaaatgat ggaacattaa tgaatttaga gtatacaatg aaatacccaa tttttacaat
     3661 tggatgtgga ataacaaatt ctataagagg agcttccttc ttatcaaaag taaaaaatgc
     3721 aatagtacta gatgttggtg gaactacaag cgacttgggt gtactatata atggattccc
     3781 tagagaatca tctattccag ttacaattgg aggagtacat acaaacttta gaatgccaga
     3841 tgttttaact attggtcttg ctggtggtac atgtgtaaaa ggagaaaaag ataatataaa
     3901 gataggtcct gaaagtgtgg gatatagaat aactgaagat gcaataatat ttggtggaga
     3961 cactctaact ttatcagatg ttgttacaaa attagatatg gcatttgaag atagaaaaag
     4021 caatgttgaa aacttagata aagagtactg tgatgaggta tataagaagg tcattttaaa
     4081 attagaggat gctgtagacc aaatgaagac tagtcaagat gatgttgaat tggttttaac
     4141 tggtggagga agtataattg ttcctgaaaa actaaaagga ataagcaatg ttataagacc
     4201 tcctcattat actgctgcaa atgctattgg tgcagcttta ggacaaataa gtggagatgt
     4261 agaaaaagta tacagtttag ataaaatgtc gagagaagat gccatagaag atgcaaaaaa
     4321 agaggctgta aataaagctc taaaagctgg tgctaaagaa gatactatag aagtattaaa
     4381 tatagaagat gttccacttg cataccttcc tggtaatgca cttcttataa aagtaaaagt
     4441 tgttggtgat ttaatttaaa gaataaatat aagtatttcc agattcgtat ttccagaatc
     4501 atataaatat ttctggactc atttttaaac ttattttatc aataaaaaca gtataacttt
     4561 tgactatatt cttataagtt tccgaacagc cgatatatac ctattttttg aggtttatcg
     4621 gctgtttgtt atcttttatc gcattccaac tatttttctt accgtatttg ctgtacgaat
     4681 tgtaatctta tcacttacat ttgaactggc agtctttgac caccaatttg tctttttata
     4741 atctttacga ctaaaagacc aaaagatagc atttttataa tgatatactt tttcatattc
     4801 ttctttagga tttccaacct catcataaaa ctcgtcataa gataagtcag gaagcataaa
     4861 aataagatta tcatatattt ctttgttgtt atctccccac caattaggag cattatttag
     4921 tatatctttt aactcctctt gtgaaataat aaaaactggt atttccaatt caaactgttt
     4981 ttttatcatt aatcttattg tatttgagat aaccttgctg tcatctacat cacttaaaaa
     5041 gacaacatta ccactattaa ggtatgtaga aacttctaaa taaccaagtt ctacaaagct
     5101 tgcttttaat tcagacataa ttatcttgtt cttaccactt atattgatac ctcttaataa
     5161 tgcgatataa cttttcatat aataatttca cctctaaaca ttcacatttt tctatgtagt
     5221 acaaactgga atttgcatct tttataattc tttcaaaact tcttcatatg taattttttt
     5281 atgctaaaaa aatcggtact taatttgtac cgatttttgt ttttcttagt aaagaactcg
     5341 tttttttgct tctgctatag ctctagcagc actttttgct tgcattcttt tttcttcttc
     5401 ttttttaaat tcatttatta tttcatttag attcttatca tgttcatttt tttctttatc
     5461 ttcgttttct ctgtatacta atacataatc tattattgat tcgtttatta tctgggatat
     5521 acttttattt tcattaattc ctattctctt aacttcctta agtagagtct catcaatatg
     5581 aaccgttgtt tgctttgtat tttttgccac cttaatcacc tcttaaaaaa aattctaaca
     5641 ttaaaatttt ttttagtcaa taaattttaa tattaaaatt ctttaaatgt tttatttgat
     5701 tttctaaaat tttctattgt atttatcatc ttatttttta tattttcacc tagtacagaa
     5761 gactttaatc taggattttc gatatggtca taaattggtt ctaaaaattc gacttcaaca
     5821 ggaacagagt taattttacc aataggttgg tatccttcat aagtatcttt tgaatttttt
     5881 attactagcg gaacaatagg gcattttgct ttatatgcta tttttaaagc tccacttcta
     5941 aactccgaaa ccaatgaatt tggttcttta atccatgtta aatctccttc tggaaatact
     6001 gccatacttt gacctgtaag tatattttga gaggcttcgg ctatactctt tataccttca
     6061 cgattgtttt ttctgtttac atatacacat cttaataatt ttgcccattc tttaaatata
     6121 ggtatatttc tccaaactgg ttcatctgca attacacatc caattggtct ttctacactt
     6181 gctgttagta tgaagctgtc aagcatacta gaatgattta ccacaaatag aagtggttct
     6241 tttggcagtg tttccttacc aattatatta agttctatat tcactatatc taaagatttt
     6301 ttagcttgtt tttgcataaa ttcaaatttt tcctttaagc taaatttatc aggattcttt
     6361 ttaattttaa taagcttagg tatagaagat gtaaaaccta taagttgata tgtagcaaat
     6421 tttaagtaat tattcataaa attatctcct taaaataaaa ttatttatta ctgtaaagat
     6481 tttaacataa aataaaattt aagtctat
//
LOCUS       DAEPXF010000193         6465 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain HB27
            SAMN13376389-rid12479423.denovo.200, whole genome shotgun sequence.
ACCESSION   DAEPXF010000193 DAEPXF010000000
VERSION     DAEPXF010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13376389
            Sequence Read Archive: SRR10518432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6465)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6465)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001069624.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001069624.1
            Genome Coverage       :: 315x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/15/2021 01:54:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,165
            CDSs (total)                      :: 4,076
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 89
            rRNAs                             :: 10, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 10 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 19 of 69
            Pseudo Genes (incomplete)         :: 44 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 10 of 69
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6465
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13376389-rid12479423.denovo.200"
                     /strain="HB27"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="China: Hebei"
                     /collection_date="2017"
     gene            complement(153..1589)
                     /locus_tag="KRL67_003315"
     CDS             complement(153..1589)
                     /locus_tag="KRL67_003315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 55 protein"
                     /protein_id="HBH1524158.1"
                     /translation="MEGRTGIKDGRWINVVERGIKNDGSYNVGPDLQDIIDEIETDFC
                     VLYFPKGKYLFTSGVKINKEIILQGDSNLSIGATQFITRGVENMSIITLTGKKQCIKN
                     INFYSDSCEIQVNEEPPTKGNPKYHYEMIINKNEKNVDMSNVSAILYDNSKNMIKGLG
                     HYENIYISGFSGTGIRIPYYSIVNDITVSSCGLGIDTGIDTIISNSRIFKCQNGMRIR
                     TGTSINNVRIEKIQKVGLISSQVKGYEGYGSYKINNITIDQCGYCGFSFGSMKDSYFS
                     GIIRRCSQYYYNTDYDTYLNIKDKVEEAYSLFYGNYFTECNIDLISDNKDNWDDGLEY
                     KHKVYVFKTLQIPKLTLRCNIDAYDYIVKSVHGGGNLMLKNNFNNYIFIDSSSPEVIN
                     GINFNYQKNGNLLRLNNDKLSYKNGKEETAIFRPNYGLIVASTDSECEQIEASYGEKW
                     EKLGSEVIGKETIYYYKHHEPDKTNQLQ"
     gene            complement(1799..3199)
                     /locus_tag="KRL67_003316"
     CDS             complement(1799..3199)
                     /locus_tag="KRL67_003316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 55 protein"
                     /protein_id="HBH1524159.1"
                     /translation="MEESIIIKDISGWINVLDYGICNDETKDVSSKLQEIIDAAPEDS
                     IIYFPKGKYLFESGVKIKKRLTLCGDSYILANVPKNTAGVTQFTFKGKKNPKSENIAI
                     ITAKSVRHCIKSICFYSDSCDRDMTSNHEPPTDKKPRFHHNVIINYRNISAIVCEDKQ
                     GEPGHYENLSFSGFSGIALDMPNNSTANDITVFTSGLGISTGENSTIANSKVWGCVDG
                     MIISTGTFINGMRVEEIKKVGIKNIGRGLNFITNITIDQCGYCGVWFDSISNVQISGN
                     ITRCGQYYYDVSYDNYLMLDNRVKEAYSLFYGNLLESSNIVLSNTNEDNWEDFKEDKH
                     KVYLIEALETKDVLLTCNIDATDWIVKSEKGNLTYNNMRQTVIFYDGKLSSINGIGIS
                     EEYYGDKVNIKKGNIYINSKDKVIKMHKPEVGDIMSTIIKDENILSAHYGGTWEKIGE
                     KIEFGITVHYYKLIEK"
     gene            complement(3226..4572)
                     /locus_tag="KRL67_003317"
     CDS             complement(3226..4572)
                     /locus_tag="KRL67_003317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 55 protein"
                     /protein_id="HBH1524160.1"
                     /translation="MENEAKTPDWINVLELNSKIDITGKYDVSNMIQNIVSDKSLEGA
                     IIYFPKGNYLFEKGIKISQQITLQGDSYYGGDSQVSNLDKKQSIIGTTNFITRGVSNM
                     SIIALTGTSQCIKNINFYYDSHDIEKIPPKNVSAITEYGETQGLFHFEHLFISGFSGI
                     GIEIPYYSTGNDIIVSSCGLGMRLGEKSMLSSSKIYECKNGMEITTSVSLNNVRIEEI
                     REIGINNKGFGFNLIMNLMVDQCGYCGFMFEKMLYSQITARITKCGQYYKSVDYNAYE
                     NMEDRKEEAYSIFYGDILENCNIVLMNSNMDTLESDLLDEHKIYVIKANETNNVMLTC
                     YAEADNFIQCAKGNLLLENGVNTYKFYNGEVCSVGGVQISDIDEKDLIKIIENTLYID
                     NKGKYLMVPRLKKNTVIYSVLDSTEEINKLHGGIWEQIDIKVLAGESMYYYKKIKD"
     gene            complement(4942..5616)
                     /locus_tag="KRL67_003318"
     CDS             complement(4942..5616)
                     /locus_tag="KRL67_003318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HBH1524161.1"
                     /translation="MNDELKSILYSKGVDIVRFVDISEFPTNQTQGFSKAILFCIGLS
                     KKFITDIYNNLPTDSDEFLEKEEKVEELADWISKYIQSKGYRAYSQSEKNNLEHGYFE
                     KAYINPEMQSGISPLPHKTIANISGIGFMGKNNLFVTEEYGCAFSMCTVLTDAPISVE
                     RYPLINSKCLNCNVCVENCPAKAIHGNEWTLPGKRESIIDVSKCFCVLKCMMSCPWSL
                     RYANQK"
     gene            complement(5677..6423)
                     /locus_tag="KRL67_003319"
     CDS             complement(5677..6423)
                     /locus_tag="KRL67_003319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2087 domain-containing protein"
                     /protein_id="HBH1524162.1"
                     /translation="MKIENLSIDDIKRGYIFNADLNCYSCIKCTKTYEVGEIYKIGNR
                     FFEASKAIELHMELEHSDYLEQLLNSDSKYNTLTENQKQLFQLFISDLPDKEIAKQIG
                     VSTSTIRHQKFIFREKAKQAKLYLALYESVFEDKSNKSSTIIPIHEHARMVDERYVVT
                     EEERKHILDTSFESMNPLKLKVFSSKEKKKVVILSKILEQFERGKKYSEKEVNQILES
                     IYDDFATIRRYLIEYGFMSRNKECTKYWLT"
BASE COUNT         2107 a         1169 c          606 g         2583 t
ORIGIN      
        1 aactaatttt ataataaaat taaaaaaggt gctattttca agcaccctac aattttactt
       61 tttaacaaat ttaatactct acaaatatat caatattaca ttaaaggtaa acactatata
      121 ttaaaacttt atatcattat ataatacaaa ttttattgta gttggtttgt tttatctggt
      181 tcatgatgtt tataataata tatagtttct tttccaatta cttcagaacc aagcttctcc
      241 catttttctc catagctagc ctctatttgt tcacattctg aatctgttga tgctacaata
      301 agaccataat taggtctgaa tatagctgtt tcttcctttc catttttata gcttaattta
      361 tcattattta gccttaacaa gtttccattc ttttgataat taaaattaat tccattaata
      421 acttctggac tagatgaatc aataaatata taattgttaa agttattttt taacattaaa
      481 ttgcctcctc catgtaccga tttaacgata taatcatagg catctatatt gcatctcaat
      541 gttaattttg gtatttgaag ggttttaaat acataaactt tatgtttata ttctaagcca
      601 tcatcccagt tatccttatt atcactaatt aaatcaatat tacattctgt aaaataattt
      661 ccataaaata aagaatatgc ttcctctacc ttatctttaa tatttaaata tgtgtcataa
      721 tctgtattat aataatactg actacacctt cttataatac cagaaaaata actatctttc
      781 attgaaccaa atgaaaagcc acaataccca cattggtcaa tcgtaatatt attgatttta
      841 taacttccat acccctcata ccccttaact tgactactta taagaccaac tttctgtatt
      901 ttttcaatcc ttacattatt tatactagtt ccagttctaa tcctcattcc attttgacat
      961 ttaaatattc tactattaga aataattgtg tctattcctg tatcaatacc cagcccacaa
     1021 gatgaaactg taatgtcatt tacaattgag taatatggta tcctaattcc tgtacccgaa
     1081 aaaccactta tataaatatt ttcataatga cctagacctt taatcatatt cttactgtta
     1141 tcatacagga ttgctgatac attgctcatg tcaacattct tttcattctt attaataatc
     1201 atctcataat gatatttagg attacctttt gtcggtggtt cttcatttac ctgtatctca
     1261 caactatcag aataaaaatt tatattttta atacattgtt ttttccctgt caatgtaata
     1321 atactcatat tttctactcc acgtgttata aattgtgtag cccctataga taaattagaa
     1381 tcaccttgca atataatttc tttattgatt ttaacaccac ttgtaaataa gtacttacct
     1441 tttggaaaat ataagacaca aaaatctgtt tctatttcat caataatatc ctgcaaatca
     1501 ggacctacgt tgtacgaacc atcattttta atacctcttt ctactacatt aatccatctt
     1561 ccatctttaa tcccagttct tccttccata atattatata ttcctttccc atttacttaa
     1621 tttgaaccta ccatgactaa agtcacaggt ttaccacttc atagaatttt acataatcaa
     1681 attaattttt tatattattt ttatattatt caatacacac tattaccaaa ccatttacat
     1741 taaaactata gtcctagctc tccaataaaa tacatctatt caaatactac gtatttactt
     1801 atttctctat caatttataa tagtgtacag ttattccaaa ctctattttt tcaccaattt
     1861 tctcccatgt tcccccataa tgagcggaaa gtatattttc atctttaata attgttgaca
     1921 taatgtcacc tacctctggt ttatgcatct taataacctt atccttacta tttatatata
     1981 tatttccttt ttttatattt actttatccc cataatattc ttcagatata ccaattccat
     2041 tgatactact tagttttcca tcgtaaaata taacagtttg tcgcatatta ttgtatgtta
     2101 aatttccttt ttctgactta acaatccagt cagtagcatc tatattgcat gttaatagta
     2161 catccttggt ttcaagtgct tctataagat acaccttatg cttatcttct ttgaaatctt
     2221 cccagttatc ttcattagta tttgacaaaa ctatattact tgactccaat aaattaccat
     2281 agaataaaga atatgcttct ttaaccctat tatccaacat taaataattg tcatagctta
     2341 catcataata gtactgccca caccttgtga tatttcctga tatctgaaca ttggatatag
     2401 aatcaaacca aactccacaa tatccacatt ggtcaattgt gatatttgta atgaaattta
     2461 aacctctacc aatattttta attccaactt tttttatttc ttctaccctc ataccattta
     2521 taaaagttcc agtagaaatt atcattccat caacacagcc ccaaacctta ctgttagcaa
     2581 tcgttgaatt ttcccctgta ctaattccaa gtccagatgt aaaaactgta atatcatttg
     2641 ctgtagaatt attaggcata tcaagtgcaa ttccagaaaa accactaaaa gacagatttt
     2701 cataatgacc tggttctccc tgtttatctt cacatacaat agctgatata tttctataat
     2761 ttataattac attatgatga aatctaggtt ttttatctgt tggaggttca tgattagatg
     2821 tcatatctct atcacaacta tctgaataaa aacagatact cttaatacag tgtcttacac
     2881 tttttgctgt tataattgct atattttcac ttttaggatt tttctttcct ttaaaagtaa
     2941 attgtgttac tccagcagtg tttttaggaa cattcgccaa aatataagaa tctccacata
     3001 aggttaatcg tttcttaatc ttaacaccac tttcaaataa gtattttcct tttggaaaat
     3061 atataataga atcttctggt gcagcatcta taatctcttg caacttggaa cttacatctt
     3121 ttgtttcatc attacaaatc ccataatcaa ggacattaat ccagccactt atatctttaa
     3181 ttattataga ctcttccatt aatcactacc tcctagtttg ataatttaat cttttatctt
     3241 tttataatag tacatggatt ccccagctaa tacttttata tcaatctgtt cccatattcc
     3301 accatgtaat ttatttattt cttctgtaga atctagtact gaatatatta cagtattctt
     3361 ttttaatcta ggaaccatta aatacttacc tttattatct atatacagag tgttctcgat
     3421 tattttgatt aaatcttttt catctatatc agaaatttga acacctccta cagaacaaac
     3481 ctcaccatta taaaatttat aagtatttac accattttct aacagcaagt taccttttgc
     3541 acattgtata aagttatctg cttcagcata acatgttagc attacattgt ttgtttcatt
     3601 tgccttgatt acatatatct tatgttcatc aagtaaatca ctttctaagg tatccatatt
     3661 actattcatc aatacaatat tgcaattttc taaaatatct ccataaaaaa tggaataagc
     3721 ttcttctttt ctatcttcca tattctcata agcattataa tcaacacttt tataatactg
     3781 accacattta gtaattctgg cagttatctg gctatagagc attttttcaa acataaatcc
     3841 acaatatcca cattggtcga ccataagatt cataatcagg ttaaaaccaa atcctttatt
     3901 attaatacct atttctctta tttcttctat tctcacatta tttaagctaa cactagtagt
     3961 aatctccatt ccatttttac attcgtaaat cttgctacta gaaagcattg acttctctcc
     4021 aagcctcata ccaagaccac atgaactaac aatgatatca ttgccagttg aatagtatgg
     4081 aatttcaatc cctatccctg aaaatccaga aataaacaga tgttcaaagt gaaatagtcc
     4141 ttgtgtttct ccatattctg taatagcaga tacattttta ggtggtattt tctctatatc
     4201 atgactatca taataaaaat taatattttt gatacattga cttgttcctg tcaatgcaat
     4261 aatactcata ttagaaactc ctcgggttat aaaattggtt gttcctatta ttgattgttt
     4321 tttatctaaa tttgatactt gactatctcc tccataataa gaatctcctt gaagtgttat
     4381 ttgttgacta attttaatac ctttttcaaa taaataatta ccttttggaa aatatataat
     4441 ggcaccttct agtgacttat cagaaacaat attttgtatc atattactta catcatattt
     4501 cccagtaata tctattttac tgtttaattc tagtacatta atccaatctg gagttttagc
     4561 ttcattttcc atgattaatt cctcctttta aatctaaatt cttttcacag aaacaaattt
     4621 ttttgattat aactgaagtg acttttactc tcttttggaa ttttgagagg acaattttca
     4681 tttttataac atcattatag ttctattata aatcaattat aatttcatta tatttttact
     4741 attaccaagt tattcttaat tttttaatac aaattttata taatgattaa aatagttaag
     4801 ctttgtatca atataattta atcccaataa aaaaacactc atttctgagt gttctataca
     4861 agtttttatt ttgagacatc ttatataatc ttatatttta aaaagtttac aatatccaat
     4921 ttaatatctt ctctagtaaa gttatttttg atttgcatat cttaatgacc atgggcaact
     4981 catcatacac ttcaatacac aaaaacactt tgaaacatca ataatagatt ctctttttcc
     5041 tggcaaagtc cactcatttc catgtatagc ttttgcaggg cagttttcta cacaaacatt
     5101 acaatttaga cattttgaat taatcaaagg atatctttca acagaaattg gagcatctgt
     5161 aagtactgta cacatactaa aagcacatcc gtattcctct gttacaaaca aattattttt
     5221 tcccataaat ccaatgccac taatatttgc tattgtctta tgtggtaaag ggctaattcc
     5281 tgattgcatt tctggattaa tataggcttt ttcaaaatat ccatgttcta gattgttttt
     5341 ctctgattga gaatacgccc tatatccttt actttgaata tattttgaaa tccaatcagc
     5401 taattcttct actttctctt ctttttccaa gaactcatca ctatctgtag gtaagttatt
     5461 gtatatatct gtaataaatt tttttgataa tcctatacaa aataaaattg ctttagaaaa
     5521 accttgcgtt tgatttgttg gaaattcaga tatatcaaca aaacgaacaa tatctacacc
     5581 tttactgtat aaaatagatt ttaattcatc attcatattt gactcctctc cctagataaa
     5641 aataacatta tacattagta aaatttatta ttttaattat gttaaccaat actttgtaca
     5701 ttctttattt ctactcataa aaccatactc aatcaaatat cttctaattg ttgcaaaatc
     5761 atcatatata gattctaaaa tctgattaac ttccttttct gaatatttct tcccacgttc
     5821 aaattgttcc aaaatcttag ataaaattac gacctttttc ttttcttttg aagaaaatac
     5881 ttttaatttt aaaggattca tactttcaaa tgatgtatcc aaaatgtgtt ttctctcttc
     5941 ttcagttact acatatcttt catctaccat tcgagcatgt tcatggattg gtattatagt
     6001 gctactttta ttagatttat cttcaaatac agattcatat agtgctaaat ataacttagc
     6061 ttgttttgct ttttctctga agataaattt ttgatggcgt atagtagatg tggatactcc
     6121 aatctgtttt gctatctcct tatctggtaa atcactaata aataattgaa ataattgttt
     6181 ttgattttca gttaatgtgt tgtactttga atcactatta agcaattgct ctaaataatc
     6241 actatgttct aattccatat gaagttcaat agcttttgaa gcttcaaaga atctattacc
     6301 tattttatat atttctccta cctcatatgt ttttgtacat ttaatgcaac tataacaatt
     6361 taaatcagca ttaaatatat agcctcgttt aatatcatca atagaaagat tttcaatttt
     6421 catgttacta cctccatttc atagtttaca tttttcataa ataca
//
LOCUS       DAEPXF010000194         6426 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain HB27
            SAMN13376389-rid12479423.denovo.201, whole genome shotgun sequence.
ACCESSION   DAEPXF010000194 DAEPXF010000000
VERSION     DAEPXF010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13376389
            Sequence Read Archive: SRR10518432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6426)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6426)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001069624.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001069624.1
            Genome Coverage       :: 315x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/15/2021 01:54:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,165
            CDSs (total)                      :: 4,076
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 89
            rRNAs                             :: 10, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 10 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 19 of 69
            Pseudo Genes (incomplete)         :: 44 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 10 of 69
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6426
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13376389-rid12479423.denovo.201"
                     /strain="HB27"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="China: Hebei"
                     /collection_date="2017"
     gene            complement(227..484)
                     /locus_tag="KRL67_003320"
     CDS             complement(227..484)
                     /locus_tag="KRL67_003320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HBH1524163.1"
                     /translation="MKIKVEDIPVQFHAMVEIIGIDKFIEVTKLYGGTNTYIPTYKGL
                     FRYARNREIVKQFNGVNHNELAIRYNMCVSNIKRILNENSV"
     gene            1064..1285
                     /locus_tag="KRL67_003321"
     CDS             1064..1285
                     /locus_tag="KRL67_003321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1659 domain-containing protein"
                     /protein_id="HBH1524164.1"
                     /translation="MEVVSTKNLSSIKLKLDAGFDDKGKAVIKSKSFANVKAEALNED
                     VYAVAEAIASLQEKPIVDILKLDSTSLSK"
     gene            1829..2059
                     /locus_tag="KRL67_003322"
     CDS             1829..2059
                     /locus_tag="KRL67_003322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2922 domain-containing protein"
                     /protein_id="HBH1524165.1"
                     /translation="MEIKKKLVMSFKNIKDKQVSFSIENPKDDLTEENIKSVMDLIVS
                     KNIFSVGGFDLTSVVEAKVVETSTTPYDLVIE"
     gene            2072..2221
                     /locus_tag="KRL67_003323"
     CDS             2072..2221
                     /locus_tag="KRL67_003323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YvrJ family protein"
                     /protein_id="HBH1524166.1"
                     /translation="MDDSLQLLITNVGFPIALSMYLLVRIEGKLNTLSNSIDTLSKNI
                     LIFKK"
     gene            2478..2915
                     /locus_tag="KRL67_003324"
     CDS             2478..2915
                     /locus_tag="KRL67_003324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family transcriptional regulator
                     DNA-binding domain-containing protein"
                     /protein_id="HBH1524167.1"
                     /translation="MKIHVEQGINCDDIEITIKCSALDNNIEKIISLLSTPLLDIKGK
                     KSGEIYKLNVCDVLYFETVGNKTFAYYEDDVFEIDLKIYKLSKLLANTSFSRISKSMI
                     LNMDYIKSIKVISHNRMQATLKNNEKVIINRRYIKNIKEKLNI"
     gene            3171..4064
                     /locus_tag="KRL67_003325"
     CDS             3171..4064
                     /locus_tag="KRL67_003325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HBH1524168.1"
                     /translation="MQLYNTPFNTNKHLIYFFTFTLVLNWIFSLTPALLGITNTILGE
                     IIFYLAYTSPAIVGLFFISTIYPQSAKQDFIYRYLSFKEIGIKWILLTILFFALTFIT
                     SLIIGNYFNVKTSDIDWRHIAVLRPSQIAYMLFESFILSILSQEAGWRGYAIDKLLVR
                     FGFIGSSIILGFACGIWYLGSYFTPNQIPYNLAQYSLFDAFLFIPNIVLLNFIINFVY
                     INTSRSILSAGLVHMTYYFFNVQLLLHYPIKLCVIAQYVQIIFSLIFLVYMISSNKFK
                     QKVNSEIEQIKSDDFEFELDW"
     gene            4242..5066
                     /locus_tag="KRL67_003326"
     CDS             4242..5066
                     /locus_tag="KRL67_003326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HBH1524169.1"
                     /translation="MFKIGEVSKLTQISIRMLRYYDELGILKPAEIDKYTGHRLYSVE
                     QISILQRIVLLRDSKFSVAEIANIVHNWNDEFVIKELNRKKNEIQKEIKQEQQRINKI
                     DKFIEAINCDKDEIHYNVVFKKIPSYKIISLREIIPDYQSEGILWEKLSKFIKEEHIE
                     VSRQSNNNIAFYHDEEVKDNGVDIEVGMVVKKIGKNKSGFIYRETEEIDMMACTMVYG
                     PYENIAGAYESFCYWLDKNSDYQISGINRQIGHKGEHNEINPENYLTEIQIPLIKV"
     gene            5164..5772
                     /locus_tag="KRL67_003327"
     CDS             5164..5772
                     /locus_tag="KRL67_003327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBH1524170.1"
                     /translation="MEKLLRGKIMKADSTKEQLITATIQLLSSHKDISSITAREIVAK
                     ANVNLAMINYYFKSKEELINISIKKFLKHHMDKYKATHDTKNLAPKQQLREILIAFFD
                     FIGEHSQFIKVSVPYILIQEEIVYPFEILPLIKAHYENQNNKKTESEYRTIAYQITSF
                     IQLLFLRSNAFFKYSGIDILNVEQRNGLIDFQLDLLLVDITK"
     gene            5876..6052
                     /locus_tag="KRL67_003328"
     CDS             5876..6052
                     /locus_tag="KRL67_003328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH1524171.1"
                     /translation="MFFTFIGVISAILLLCTIICGLWIKLNNRNNDSNSHFTFSILSI
                     AFSLLIVVAFLVRN"
BASE COUNT         2489 a          788 c          780 g         2369 t
ORIGIN      
        1 tttatctatt ttttagccaa caaatatttt cgactatttt ctttgtattc tcaatatatc
       61 tccagtatct aacaaatttt tttatagaat atacaatgta tttaaattaa taagtaataa
      121 atttagttaa tttgtctaaa aaaactttat aaaacatctt cgttgtcttt actcttacat
      181 ttaataaggc tattttcatt atgtttaaac attattttta ttatatttaa acactatttt
      241 cattgagtat tcgcttaata ttgcttacac acatattata tctaatagcc aattcattgt
      301 ggttgactcc attaaattgc tttactatct ccctatttct agcatatcta aaaagtccct
      361 tatacgtagg tatataagta tttgttcctc catacaactt ggtaacctct atgaacttat
      421 caattccgat tatttctacc attgcatgaa attgaactgg tatatcctcc acttttatct
      481 tcataagtta cacctccttt aagatatttt tatagactta acaaagtttt tatttaatac
      541 attattgact aaactatatt tattagtttt attgaatcta ataaaaaatt tcttagcttt
      601 aaaattaatt tctgtattaa ctttaataaa atttttcatt acttataact ttgttagctt
      661 taataaagat tttaacggtt taaaagtaaa atgttaatat tggactttta acttttttat
      721 ttacagatga aatttaataa aatttttaac ataaacttta acatttaatt taacataaac
      781 tttagcatct aacatttaac tctacacata taaaaatcca tacaaaacta cctcaactaa
      841 ataggaatat atgcaaatac tatttcaaaa cactaacaac atatcttcac aaataactta
      901 taaatttttt catcatcaaa aaagtaaaat ttttcctata aaaatttaca caaaaaatct
      961 ttagaaatta ctgtttaact ccgatattat cttagatgat agtattatag ctaacaaaga
     1021 aagtcgacaa atctttgaaa ttaaattttt aggaggtaat tttatggaag ttgtttcaac
     1081 aaaaaattta tctagtataa aactaaaatt ggatgctggt tttgatgata agggtaaggc
     1141 agttattaaa agtaaatcat ttgcaaacgt aaaagcagag gcattaaatg aggatgttta
     1201 tgctgttgca gaagcaatag caagtcttca agagaagcct attgtagata ttttaaaatt
     1261 agatagtaca tcactatcta aataatatct tttaaacgta atctgcttta caaattaaaa
     1321 aaattaactc tttatataaa agattaagtt atataacgca cttaatactc tgtagatttg
     1381 gcaggttata acttcaattt agaatataac tttaatatat tgatacttta aaaattaata
     1441 tattaaatgt atttcaactt aaacatgtta gctattttaa atgctaccct acttcaatct
     1501 aattaattga aataaattaa atattttcta tatcagcaat aactagtgtc aacaattaaa
     1561 acactgatac taacaaacag aacactaata ttgacaattg aaatattaat actaacaagc
     1621 aaaacactaa tattgacaat tgaaatacta atactaacaa gcaaaacact aatattgaca
     1681 attgaaatac taatactaac aagcaaaaca ctaatattga caattgaaat actaatacta
     1741 acaagcaaaa cattaatatt gacaattaaa ataccaatat taacaatcaa acaattaaaa
     1801 tataaattat tatatcggag gtaattatat ggaaattaag aaaaaattag tcatgagttt
     1861 taaaaatata aaggataaac aagtttcttt ttctatagaa aatccaaagg atgatttaac
     1921 tgaagaaaat ataaaatctg tcatggattt gatagtatct aaaaatattt tttcagttgg
     1981 aggatttgac ctaacttctg ttgttgaagc caaggttgtg gaaacaagta ccactcctta
     2041 tgacttagtt atagaatagg aggctaattt tatggatgat agtttacagc tgttaataac
     2101 caatgttggt tttcctatag ctctatccat gtacttgttg gttagaattg agggtaaatt
     2161 aaatacttta tcaaacagta ttgatacatt atccaaaaat attttgatat ttaagaagta
     2221 atttgacaat atatgtaagt cataaaatag gtctatttgt aaaataatta gatatattct
     2281 atgcgaaata ctaggatatt tggataattt atccaaatat ccatagtatt tttttgaatt
     2341 ttaccacctc aaatttgaat ttcactattt tatttttgaa tttcactatg cttaatttga
     2401 aatttagaac gcatctagtt ggactcaata agatataatg ctattctaat gacagtaata
     2461 aaatgaggtg atgattaatg aaaatacatg tcgaacaagg tattaattgt gatgatattg
     2521 aaataaccat aaaatgttct gctctagata ataacattga aaaaataatt tcattattga
     2581 gtacaccatt acttgatata aaaggtaaaa aatcaggaga gatttataag ctcaatgtct
     2641 gtgatgtctt atacttcgag acagttggaa ataaaacatt tgcttattat gaagatgatg
     2701 tatttgaaat tgatttaaaa atatataaat tatcaaaatt attagctaat acatctttct
     2761 cacgaatatc caaatccatg attttaaata tggactacat aaaaagtata aaagtaatat
     2821 cacataatcg catgcaagct actttaaaaa acaacgaaaa agttattata aatcgtcgtt
     2881 atataaaaaa tattaaagaa aagctaaata tttaataaaa atctaaagta atttattaat
     2941 atcactaatt tcaaatttat tgacctattc aataattaaa ataatataat atgttcaata
     3001 ttaaatgcta attggttata tttagtgtaa aaataattaa tcaaaatata aaaaatagga
     3061 tattactgta atcaataact aacatcaaac gataacttaa tacatataga aataaataaa
     3121 caattaataa aaattaaaac ttcattataa aacggaagga tagtgaaaaa atgcaactat
     3181 ataacacccc atttaataca aacaaacacc taatctattt ctttacattt actctagtat
     3241 tgaactggat attcagtctt acacctgcat tactcgggat tacaaatacg atacttggag
     3301 aaatcatttt ttatcttgca tatacatcac cagctattgt aggtctattt tttatttcta
     3361 caatctatcc acaaagtgca aaacaagatt ttatttatag atacctaagc tttaaagaaa
     3421 tcggaataaa atggatatta ttaacaatat tattttttgc tttaactttt attacttctt
     3481 taataatcgg aaattacttt aatgtaaaaa cttctgatat agattggaga cacatagcag
     3541 tgcttagacc ttctcaaata gcttatatgc tatttgaaag ttttatatta agtatactta
     3601 gtcaagaagc tggatggaga ggatacgcca ttgacaaatt attagttaga tttggattta
     3661 taggctcttc aattatttta ggatttgctt gtggaatctg gtatctaggg tcgtatttta
     3721 cacctaatca aataccatat aatttggcac aatactcact atttgatgct tttttattta
     3781 taccaaacat tgtactttta aactttataa ttaattttgt atatatcaat acaagtagaa
     3841 gtattttatc agctggatta gttcatatga cgtactattt tttcaacgtt caattgcttc
     3901 tacactaccc tattaaatta tgtgtaatag ctcaatatgt acaaataata tttagtttaa
     3961 tatttcttgt atatatgatt tcttctaata aatttaaaca gaaggtaaat tctgaaattg
     4021 aacagataaa gtcggatgat tttgaatttg agttagactg gtaattttaa tatgaattat
     4081 aaattaagat aatctatgat ttgacacgat tactacttaa atataattta ctctctataa
     4141 tttattgcaa catctggaaa attattgtat ttccacaaac tcttgaagta tataaaaaca
     4201 aaatatatac tgattataag ttaaaaggag gtcatatttt catgtttaaa ataggagagg
     4261 tttcaaaact aacacaaatt tctattcgta tgttgcgtta ttatgatgaa cttggtattt
     4321 taaagcctgc agaaatagac aaatatacag gtcatagatt atactcagtt gaacagattt
     4381 caatactaca aagaattgta ttattgagag attcaaaatt tagcgttgca gaaatagcaa
     4441 atatcgtaca taattggaat gatgaatttg taattaagga attgaataga aaaaagaatg
     4501 aaatacaaaa ggaaataaag caggaacaac aaagaataaa taaaattgat aaatttatag
     4561 aagctataaa ctgtgataaa gatgaaattc attacaatgt tgtatttaag aaaattccaa
     4621 gttataaaat catatcactt agagagataa tcccagacta ccaatctgaa ggtatactct
     4681 gggaaaagtt atctaaattt attaaagaag aacatataga agtatcacgt caatccaaca
     4741 ataatatagc tttttaccat gatgaagaag tcaaagataa tggtgtagat attgaagttg
     4801 gaatggttgt aaagaagatt ggaaagaaca aatcaggatt tatatatcgc gagacagaag
     4861 aaattgatat gatggcatgt accatggtgt atggtccata tgaaaatata gctggtgcat
     4921 atgaatcgtt ctgttattgg ttagataaaa atagtgatta tcaaatatca ggtataaata
     4981 gacaaatcgg tcataagggt gaacataatg aaataaatcc tgaaaattat cttacagaaa
     5041 ttcaaatccc ccttataaaa gtctaatcga ttagtactta aatacttaag tactaatcac
     5101 ccattgacat cccaaaattt tcatgatagt atctaaacgt gtattaaaca tgtttaattt
     5161 tttatggaga aacttttaag gggaaaaatt atgaaagcag attcaacaaa agaacaatta
     5221 attactgcca caatccagtt actatcttca cataaagata tttcaagcat tacagctaga
     5281 gaaattgtag ctaaagcaaa tgtcaattta gctatgatta attattattt taaatctaaa
     5341 gaggagttaa ttaatatttc tattaaaaaa tttttaaagc atcatatgga taaatataag
     5401 gctacacatg atactaaaaa tttagcacct aaacagcaat taagggaaat actaattgca
     5461 ttttttgatt ttataggtga acatagtcaa tttataaagg tttctgtacc ctatatattg
     5521 atacaagaag aaattgttta tccatttgag atactacctt taattaaggc tcactatgaa
     5581 aatcagaata acaaaaaaac tgaatctgaa tacagaacta ttgcgtatca aataacttca
     5641 tttattcagt tgctttttct tcgttctaat gcttttttca agtatagtgg cattgatatt
     5701 cttaatgttg agcagagaaa tggcttaata gattttcaat tagatttatt attggtagat
     5761 attacaaaat aatactatat taatttttta tgtacatttt tttattttat ggtaacgctt
     5821 gaaaatattc tcattgacta aaaatatttg cattagataa gggggaggaa ttttaatgtt
     5881 ttttacattt ataggagtaa tatcagcaat tttattgttg tgtacaatta tatgtggttt
     5941 atggataaaa cttaataatc gtaataatga ctctaattct cactttactt tttcaatact
     6001 ttcaatcgcg ttttcactgt tgattgtggt agccttttta gtaagaaatt aatgtcttta
     6061 ttatgttggg gtttattatt atagattttt attattgcat gattttgtca tattacatca
     6121 tttttattat attacagagt cttatttatt acaggatttt tattatattg taaagactta
     6181 tttattacag tgctttgtta tattacagac ttttatatta caacattttt attatattac
     6241 aagctcttat ttattacagt gtttcgttat attacagact tttattatta caatattttt
     6301 attatattac aagctcttat ttattacaga cttttattat tacaacttta ttatattaca
     6361 agctcttatt tattacagtg ttttgttata ttacagactt ttattattac aatattttta
     6421 ttatat
//
LOCUS       DAEPXF010000195         6418 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain HB27
            SAMN13376389-rid12479423.denovo.202, whole genome shotgun sequence.
ACCESSION   DAEPXF010000195 DAEPXF010000000
VERSION     DAEPXF010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13376389
            Sequence Read Archive: SRR10518432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6418)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6418)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001069624.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001069624.1
            Genome Coverage       :: 315x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/15/2021 01:54:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,165
            CDSs (total)                      :: 4,076
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 89
            rRNAs                             :: 10, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 10 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 19 of 69
            Pseudo Genes (incomplete)         :: 44 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 10 of 69
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6418
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13376389-rid12479423.denovo.202"
                     /strain="HB27"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="China: Hebei"
                     /collection_date="2017"
     gene            142..1056
                     /gene="queG"
                     /locus_tag="KRL67_003329"
     CDS             142..1056
                     /gene="queG"
                     /locus_tag="KRL67_003329"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HBH1524172.1"
                     /translation="MDNKELKAFCNLIGLNCVGVAGVDKYDNLEKILKDRQQKGYLTG
                     MEEPIIENRINPRHIMKDANSIIVCAFPYHINRQEKNKNSNLSKYCHGKDYHIVVKDF
                     LQQICDYISKTVSDFKYKLFADNGPLVDRYLAYLSGIGYFGINNNIITDEYGSYVFIG
                     YIVNNYKFEEDKPLEKSCFKCGKCVKYCPGNAILGNYDMNPKKCLSYITQKKGDLSEK
                     EKVVLKNGKKIFGCDICQEVCPHNVEIPTTHILEFKENIIDYLDYDEIQNISNKEFKR
                     RYGDRAFSWRGRNIIKRNMEIILDKYDD"
     gene            1162..3399
                     /locus_tag="KRL67_003330"
     CDS             1162..3399
                     /locus_tag="KRL67_003330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBH1524173.1"
                     /translation="MKYNNTSEINPLDCILGQERAVEAMELGLKINNPVYNIYIAGEP
                     GTGKSTYALKVLNEYASKKNTHKDWCYIYNFENPREPIIVELEKGFGRELKKDMEKLI
                     ESLLEDFKNAFESENFEIEKNKLLDEYEIEKDMLLKQIKKYGEEKGFKLKQSKMGIVF
                     IPLKEEEDESDESDEEFYKAKRELENMAIQVVYKIRNLEEIAEKAVLELEEEIAKLVV
                     EPHIKRLCEKYENYDKVQTYLENINKDIIEYMYLFYLDEEELKDKYDKEHFIKYKINL
                     FVDNGSSKNKESAPVVVEINPSPANLFGKAEYDYANGNIKTDFTKLLSGAFHRANGGY
                     LVLYADQLLKYTMSWEILKKTLQTKKVILETQTAIKPENMPLDVKLVLIGSHYIYDIL
                     YRYDEDFEKYFKVFVDFDSEMDKNEDNEEGIARFIASQCNKNDLRHFTKSAVEEVIKF
                     STRLSGDLEKLSTKFNKIMEVVIEGSAYAEFRNSEYTEQCDVKKAINEKRKRINRVET
                     HMDESIENGFTLIETEGRKVGVINGLSVLSTGEYSFGRASRITATTSPGSKGIVNIER
                     EVNMSGSIHNKGVLILGGYLSENFAQDLLLSLNAYVCFEQNYGGIDGDSASSAELYVL
                     LSSLSGVSIKQNIAVTGSINQKGDIQVVGGISEKIEGFYSICKTKGLNGEQGVIIPRK
                     NQRNLVLSDEVNDAVRDGKFKIYTVERVEEAIEILTDVKFEEIKKLVKEKLISFSKIQ
                     TVSKE"
     gene            3596..4648
                     /gene="nth"
                     /locus_tag="KRL67_003331"
     CDS             3596..4648
                     /gene="nth"
                     /locus_tag="KRL67_003331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HBH1524174.1"
                     /translation="MKNVNLKEINEKDVIVEEEINKKGMDNKDIIVEEEINKKSIDKK
                     DIIEKEEIDKKNVKKVEVQKGIDKMIGKKDEIIDEKDIENFMDIEEDKKSIKVSKKST
                     KKDENKKKTKKNSSSKATQKPSKETKSKSKIKNKSANEKDVNKILDELEKLYPDAKCE
                     LNYGTAFELLIATILSAQCTDVRVNKVTSELFKKYNTARDFANLSIEEISKEIKSCGL
                     YKSKSQKIKDTSEQLCELYDGEVPDSLEKLIKLPGVGRKTAGVVLSNAFNHPAIAVDT
                     HVFRVSNRIGIVDEPNPQKTEFALMEAIPKERWSHSHHVLIFHGRRMCKARNPECASC
                     PIKEDCNYYKELNETK"
     gene            4842..5312
                     /gene="trmL"
                     /locus_tag="KRL67_003332"
     CDS             4842..5312
                     /gene="trmL"
                     /locus_tag="KRL67_003332"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HBH1524175.1"
                     /translation="MSLNIVLVEPEIPQNTGNIIRTCAATGSTLHLVKPLGFSLDDKH
                     LKRCGLDYWDIADIQYYDSFEELQEKYPNSKYFFSTTKAKQSHSDVKYEENCFIVFGK
                     ETKGLPEPLLKANMDTCIRVPMLDVEKARSLNLSNSVAIVVYEALRQIGYPNMR"
     gene            5348..6196
                     /locus_tag="KRL67_003333"
     CDS             5348..6196
                     /locus_tag="KRL67_003333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HBH1524176.1"
                     /translation="MKNLKIICDSLSDVPKDLLDKYDIDIVSLTVILEEREYKDRIDI
                     SGDEFYKRLREENVYPKTSQATYAQFKEIFEKYTNEDREILYIAGSATATGTYQSAVM
                     AKNDVDGKIYTYDSNLLCMGTGLLVIKAGELASEGKSVDEILPVLDELKEKAFVIFSV
                     DTLEYLQKGGRISSTKAAIGSILNIKPILEIRDGLVSQISQVRGKKNVISKMIELIKS
                     NCGDDLSDQIVYIGYSDDFKEKEQLSKVMQDELRPKEIRFFQIGVCIGAHAGPGVTGI
                     ICLKNK"
BASE COUNT         2699 a          574 c         1215 g         1930 t
ORIGIN      
        1 attaagtatt taaaagagtt agtatataaa aaagttaaat aaataaaaaa aactaagcat
       61 ttaaaagagt taagtagcta gaaaaggtta aaaaaattaa ataaatttga aaagttaatt
      121 ggattaaaaa ggaataattt tatggataat aaggaattaa aagcattttg taatttaata
      181 ggattaaact gtgtaggagt agcaggggta gataagtatg ataatttaga aaagatactt
      241 aaggacagac aacaaaaagg ttatttaact ggtatggaag aaccaataat tgagaacaga
      301 attaacccaa gacatataat gaaggatgct aactctataa tagtgtgtgc atttccatat
      361 catataaata gacaagaaaa gaataaaaat tcaaatttat caaaatattg tcatggaaag
      421 gattatcata ttgtagtaaa agacttttta caacaaattt gcgattatat ttctaaaaca
      481 gtatcagatt ttaaatacaa gttatttgct gataatggac cattagttga caggtattta
      541 gcttatttat caggaatagg atattttgga ataaataata atatcataac tgatgaatat
      601 ggttcttatg tatttatagg gtatatagta aataattata aatttgaaga agataaacca
      661 ctagaaaaaa gttgttttaa atgtggtaag tgtgtaaaat attgtccggg aaatgctata
      721 ttgggaaatt atgatatgaa tcccaaaaaa tgtttatcat atataactca aaaaaaagga
      781 gatttgtcag agaaagaaaa agttgtttta aagaatggca aaaagatatt tggatgcgat
      841 atttgtcaag aagtatgtcc acataatgtt gaaataccaa caactcatat tttggaattt
      901 aaagaaaata taatagatta tttggattat gatgagatac aaaatatttc aaataaagaa
      961 tttaaaagga gatatggaga tagagctttt agctggagag ggagaaatat aataaagaga
     1021 aatatggaaa taattttaga taagtatgat gactaataat aagagtctgt ttcatatact
     1081 ttaatgtgta tttactttaa cacttgttgt gtaaaagtca tattatatca ttaaagttaa
     1141 atatggggag gtgggggaat attgaaatat aataatacat ctgaaataaa tccactagat
     1201 tgtatattgg gtcaagaaag agctgtagaa gctatggaat tagggttgaa aataaataat
     1261 ccagtgtaca acatatatat agctggtgaa cctggaactg gtaaaagtac ttatgcatta
     1321 aaggttttaa atgaatatgc atccaaaaaa aatacgcata aagactggtg ctatatatat
     1381 aattttgaaa accctagaga gcctattata gttgaattag aaaaggggtt tggaagagaa
     1441 cttaagaaag atatggagaa attaattgaa agtcttttag aagattttaa aaatgcgttt
     1501 gaaagtgaaa attttgaaat agaaaaaaat aaacttctgg atgaatatga aatagaaaaa
     1561 gatatgcttt tgaagcaaat taagaaatat ggagaagaaa aaggatttaa attaaaacag
     1621 agtaaaatgg gaatagtttt tatccctttg aaagaagagg aagatgaaag tgatgagtct
     1681 gatgaagagt tttacaaagc taaaagagaa ctagaaaata tggcgataca ggtcgtatat
     1741 aaaattagaa acttagaaga aatagctgaa aaagcagtgt tagagcttga agaagagata
     1801 gctaaacttg tagtagaacc tcatatcaag aggttgtgtg aaaaatatga aaattatgat
     1861 aaggttcaaa cttatctgga aaatataaat aaagatataa tagaatatat gtatctgttc
     1921 tatttagatg aagaagagtt aaaagataag tatgataaag agcattttat taaatataaa
     1981 atcaacctat ttgtagataa tggaagctca aaaaataaag agtcagcacc tgttgtagta
     2041 gagataaacc caagtccagc aaatctattt ggaaaggcag agtatgatta tgcaaacgga
     2101 aatataaaga ctgatttcac aaaactacta tcaggagcat ttcatagggc taatggtggt
     2161 tatttagtac tttatgcaga ccaattacta aaatatacaa tgtcttggga aattcttaaa
     2221 aagactttgc agactaaaaa agtaatattg gaaacacaaa cagcaattaa accagagaat
     2281 atgccattgg atgttaagtt agtactgatt ggaagccact atatatatga tattttatat
     2341 agatatgatg aagattttga aaaatatttt aaagtatttg tagactttga tagtgaaatg
     2401 gataaaaatg aagataatga agagggaata gccagattta tagcatccca atgtaataag
     2461 aatgatttaa ggcattttac taaatcggca gttgaagagg ttattaaatt tagcactaga
     2521 ttatctggtg atttagaaaa actatcaact aagtttaata aaataatgga agttgtaata
     2581 gaaggaagtg cttatgcaga atttagaaat tcagagtata cagagcagtg tgatgttaaa
     2641 aaagcaataa atgaaaagag aaaaagaatc aatagagtgg aaacacatat ggatgaatca
     2701 attgaaaatg gatttactct aatagaaaca gagggacgaa aagtaggagt gataaatggt
     2761 ctgtcagtct taagtacagg agaatactct tttggtagag cttcaaggat tacagcgacc
     2821 actagcccag gaagtaaagg catagtaaat atagaaagag aagtaaatat gagtggctct
     2881 atacataaca aaggagtgct tatattaggt ggctatcttt ctgagaattt tgcacaggat
     2941 ttactattgt cactaaatgc atatgtttgc tttgaacaaa attatggtgg aatagatgga
     3001 gatagtgcat cttctgccga attatatgta ctattatcat ctcttagtgg agtatcaatt
     3061 aaacaaaata ttgctgttac aggttctata aatcaaaagg gtgatatcca agtagtaggt
     3121 ggtataagtg aaaagataga aggattttat tctatatgta aaactaaagg gttaaatggt
     3181 gagcaaggcg tcataattcc tagaaaaaat caaagaaatc tcgttttatc agatgaagtt
     3241 aatgatgcag tgcgagatgg aaaatttaag atatatactg ttgaaagagt agaagaggct
     3301 atagagatat tgacagatgt taaatttgaa gaaataaaga aactggtaaa agaaaaatta
     3361 atatctttca gcaagataca aactgtgtca aaagaataag tttattatag aatttaatgg
     3421 gtatttatgt ataataaaaa gcaagaactt taataggttt ttgctttttt tgtaaatata
     3481 tataagttaa attcttattt tgtatgtttt atatattgat gtttattttt tgttgatatg
     3541 actaaactat ttataaatag tgttttataa attggtattt taaaggggga taattatgaa
     3601 aaatgtaaat ctaaaagaga taaatgaaaa agatgtaatt gtggaagaag agataaataa
     3661 aaaaggaatg gataataaag atataattgt agaagaagag ataaataaaa aaagtataga
     3721 taagaaagat ataattgaga aagaagagat agataaaaaa aatgtaaaga aggtcgaggt
     3781 tcaaaaagga atagataaaa tgattggtaa aaaagatgaa ataattgatg agaaagatat
     3841 agagaatttt atggatattg aagaagataa aaaaagtatc aaagtaagta aaaaaagtac
     3901 taaaaaagat gaaaataaga aaaaaacaaa gaagaatagt tctagtaagg ctacacaaaa
     3961 accaagtaaa gaaactaaaa gtaagtctaa aattaaaaat aagtcagcaa atgaaaaaga
     4021 cgtaaataag attttagacg agttagaaaa actgtaccca gatgctaaat gtgagttaaa
     4081 ttatgggaca gcctttgaac ttttaatagc aactatttta tcagcacaat gtactgatgt
     4141 aagggtaaat aaagttactt cagagttgtt taaaaagtac aacacagcta gagactttgc
     4201 taatttgagc attgaagaaa ttagtaaaga gataaagagt tgtggtcttt acaaaagcaa
     4261 gtcccaaaaa ataaaggata catctgaaca gctatgtgaa ttatatgatg gtgaagtacc
     4321 tgatagttta gaaaagctta taaaattacc tggtgttgga agaaaaacgg caggggttgt
     4381 acttagtaac gcttttaatc atccagcaat agcagtagat actcatgtat ttagagttag
     4441 caatagaata ggaatagtgg acgagccaaa tcctcaaaaa acagaatttg cacttatgga
     4501 agctatacca aaagaaagat ggtcacactc acatcatgta ttaatttttc atggaagaag
     4561 aatgtgtaaa gctagaaatc cagaatgtgc aagttgtcca ataaaagaag attgtaatta
     4621 ttataaagaa cttaatgaaa cgaaataact tttagaaagt aagttgtaca agggggattt
     4681 tctgatataa taatacttgt gaaaatgtta gataaaaaac tattatatac atgaaataaa
     4741 aattattatt aacattattt tatataatag tataatatta ttgttattct gtttataaaa
     4801 taaaatttga taaataaaag cgatacttag ggaggattaa aatgtcatta aatatagtat
     4861 tagtagaacc agaaatacct caaaatacag gaaatataat aagaacctgt gcagcaacag
     4921 gttcaacttt acatttagta aaaccactag gtttttcatt ggatgataaa cacctaaaaa
     4981 gatgtgggct tgactattgg gatatagctg atatacaata ttacgatagc tttgaagaat
     5041 tacaagaaaa gtatccaaat agtaaatatt ttttttctac aacaaaggct aaacagtcac
     5101 attcagatgt gaaatatgaa gaaaattgtt ttatagtatt tgggaaagaa acaaagggat
     5161 taccagaacc tttgttgaaa gcaaatatgg atacttgtat tagagtgcct atgttggatg
     5221 ttgaaaaagc taggtcatta aatttatcaa attcagttgc tattgtagtg tacgaggctt
     5281 taagacaaat aggataccca aatatgaggt agtttcaaca agcctaaatg agaaaggagc
     5341 atacttaatg aaaaatctaa agataatctg tgacagttta tcggacgtgc caaaagattt
     5401 gttagataaa tatgatatag atatagtttc attaacagtt atactggagg aaagagaata
     5461 caaggataga attgatatat ctggagatga attttataaa aggcttagag aagaaaatgt
     5521 atatccaaaa acttctcaag ctacttatgc ccaatttaag gaaatttttg agaaatatac
     5581 aaatgaggat agagagatac tttatatagc agggtctgcc acagctacag gaacgtatca
     5641 aagtgctgta atggcaaaaa atgatgtaga tggtaaaata tatacatatg actctaactt
     5701 gctttgtatg ggaactggtt tacttgtaat aaaagcagga gaacttgcaa gtgaaggaaa
     5761 gtctgttgat gaaatattgc ctgttttgga tgaattaaaa gagaaggctt ttgttatatt
     5821 ttcagtagat acattggaat atttacaaaa gggaggtaga atatcatcta ctaaggcagc
     5881 aataggaagt atattaaata taaaaccgat tcttgaaata agagatggat tagtttcaca
     5941 aatatctcaa gtaagaggaa agaagaatgt aatttctaag atgatagaat tgataaaatc
     6001 aaattgtggg gatgatttat cagaccaaat tgtgtatata ggatatagtg atgattttaa
     6061 ggaaaaagaa caattatcta aggttatgca agatgaatta agacctaaag aaattagatt
     6121 tttccaaatt ggtgtatgta taggtgcaca tgcgggtcct ggtgtcactg gtatcatatg
     6181 cttaaaaaat aaataatata tttttggatg acattattta tttttttatt aaattaggat
     6241 agaatattaa ttattgagat gttaaattgg atttattttg gatgaaatta agtgttgata
     6301 tattagattt agtttgtagg gaagtgtgta aatagataag aatctattgc acactttttt
     6361 atagtaagat ttattaaaaa aatttcaaaa tgtacttgta aattgtatat aaaaaatt
//
LOCUS       DAEPXF010000196         6396 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain HB27
            SAMN13376389-rid12479423.denovo.203, whole genome shotgun sequence.
ACCESSION   DAEPXF010000196 DAEPXF010000000
VERSION     DAEPXF010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13376389
            Sequence Read Archive: SRR10518432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6396)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6396)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001069624.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001069624.1
            Genome Coverage       :: 315x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/15/2021 01:54:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,165
            CDSs (total)                      :: 4,076
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 89
            rRNAs                             :: 10, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 10 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 19 of 69
            Pseudo Genes (incomplete)         :: 44 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 10 of 69
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6396
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13376389-rid12479423.denovo.203"
                     /strain="HB27"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="China: Hebei"
                     /collection_date="2017"
     gene            complement(95..703)
                     /locus_tag="KRL67_003334"
     CDS             complement(95..703)
                     /locus_tag="KRL67_003334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HBH1524177.1"
                     /translation="MVDNLIKYIHKSDVAIVVLHEIYGINSFIEDVCQKYYKYGYDVF
                     CPELLGEKRVFPYSKVQEAYDFFINNVGFDIYKKVEKLINDLKSEYKYVFIAGYSVGA
                     TIAWRCSEDSFCDGIICCYGSRIRDYMDVIPKCPTLLVFAKYDSFDVDSISYQLSRKQ
                     NIQIEILDANHGFIDIYSNNYSDIQTKVFTIKEKSFLSKCLA"
     gene            complement(929..1633)
                     /locus_tag="KRL67_003335"
     CDS             complement(929..1633)
                     /locus_tag="KRL67_003335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HBH1524178.1"
                     /translation="MFNIELENKEYKLLCKNYSKNDGVKAIFLYLVIMLVLFLQGYLY
                     KTNLSPTILNVLQGIFSTLILFIGLGFVLFSKEKLNTIGITRKNLKQSLFYGILGATI
                     LIIFIILFNKVIEGKKVVFLFPTITTAITFIVTAISEEVVFRGYIQTRLTGLIQNNII
                     TSCINAFLFLSTHYPVRWAASGFSVTILSGFYVVCLILLHFVCDLVYRKTNCIWGSYL
                     LHVLYNVFTSILIFSI"
     gene            complement(2028..2573)
                     /locus_tag="KRL67_003336"
     CDS             complement(2028..2573)
                     /locus_tag="KRL67_003336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH peroxidase"
                     /protein_id="HBH1524179.1"
                     /translation="MKKFVCTVCGYIHEGDAAPAQCPVCKVGADKFEEMKGEKNWADE
                     HRIGVAQGVDEEIIEGLRANFVGECTEVGMYLAMSRQADREGYPEVGEAYKRIALEEA
                     EHAAKFAELLGEVVVADTKENLRVRVDAEYGATDGKLKLAKKAKELGLDAIHDTVHEM
                     CKDEARHGKAFLGLLNRYFGK"
     gene            complement(2691..4514)
                     /locus_tag="KRL67_003337"
     CDS             complement(2691..4514)
                     /locus_tag="KRL67_003337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBH1524180.1"
                     /translation="MNLSTGNQKVSRLKKKEKPKDFKKTFKRIFMYFKKDKKITRFIF
                     LLVIIDSIISTVIPYTIGKVVDIIDIDTYSKSIFLKGIIILLSFYIIEFSVKLSKGIC
                     TARLSQSIVKEMRSNLFLKFKNLPITFFDKSSTGDIMSRVTNDVDNISTGISNSLVQL
                     ITGILNILMTFIMMFVLSPILTFCSIILIPIVMFVSNFIAKRTRVFFKQQQVELGKLN
                     AHIEEMISNINVVKSFNYERKSVNDFKEVNDRLLNISLKSQIYTSLLMPIMNVISNIG
                     FATICIIGGVLASKDIITIGVIASFLSYVRQFTRPLNELANLFNTLLSAIAGCERVFE
                     IIDEQEEQEVDNVFKSELNDNKDGSLNSHFVKGEIELKDVSFSYDSKKKILDNINLKI
                     KAGTSNAIIGETGSGKTTIINLITNFYHIDEGSIFLDGKDIYSIDRNYLRTCFGVVPQ
                     EGYIFNDTVMENIRYGNLEATDEEVIYASKIANSHKFIVKMREGYSTVLSDRGEDLSE
                     GQKQLISIARAVLKNAPILILDEATSNIDIATEAKIQEAINNLTYGKTSIIIAHRLST
                     ISNCDNIIVLENGRIVEYGNHKELIKLKGHYYRNILLTQGK"
     gene            complement(4527..6302)
                     /locus_tag="KRL67_003338"
     CDS             complement(4527..6302)
                     /locus_tag="KRL67_003338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBH1524181.1"
                     /translation="MRYFKKYIKKYIVLFLTAVFFLTLEAFCDLAQPTIMAKIIDIGV
                     SNKNLNYILSTGAIMIGITFLGAIFAIIRSVISARVSQHFSYDLRQDVFEKINTYSLK
                     QIDKFGRASLITRITNDINQMQQFVNGMMRVFIKSPIMCIGSLIIAIKLNLKMSIIIL
                     IAVLIIFIIILLNMKIGYRLFKNVQESTDKVNSKLRQFLSGIKVVKLFCRYDYENQSF
                     EELNDELFKNSRKATTMMSFFRPTITIIINVSIIIILLIGRHLIYSGEIKIGIIVAYV
                     NYMTRILTSLIMISHVFNVFVRAKASYERISEVLVDENEEVIERDESLDVGEKDLKFV
                     NCDIIFENVDFSYNKNTDEKILKNISFKIEEGQNIGVIGQTGSGKTSLVNLIQKLYII
                     DKGDIKIGGRSIYSISEESLRERIGVVFQNSSVFGKSILENVVMGNRDICKEDVIKSC
                     EIANANQFITKLENTYDEVLFQRGKNLSGGQRQRITIARAIAKKPQILILDDCFSAMD
                     INTETIIRKNIKEYISNLREKESKKCTVITISQKISSIIDCDKILVLDEGEVVGFATH
                     EDLLVSSPIYKKIFALQNLVSEDVN"
BASE COUNT         2193 a         1087 c          571 g         2545 t
ORIGIN      
        1 tataataaca ctactaatat tttaaatatc aatagtgtca taaaaatttt ttcagattaa
       61 ttaaatcttt ttatatattt tataataatc caaatcatgc taaacattta gacagaaaac
      121 ttttttcttt aatagtaaat acttttgttt gaatgtcaga ataattattt gaatatatat
      181 ctataaaacc atgatttgca tctaaaatct caatttggat attttgtttc ctagaaagtt
      241 ggtaagaaat agaatcaaca tcaaatgaat cgtactttgc aaatactaat aaggtaggac
      301 attttggaat tacatccatg taatctcgaa tccgagaacc ataacaacaa attatgccat
      361 cacaaaaaga atcttcactg catctccatg caatagttgc tcccacgcta tacccagcaa
      421 taaatacata tttatactct gattttaaat catttataag cttttcaact tttttataaa
      481 tatcaaaacc aacattattt ataaaaaaat catatgcctc ttggactttt gaatatggaa
      541 aaactctttt ttctcccaga agttcaggac aaaatacatc atatccatat ttataatact
      601 tttgacaaac atcttctata aagctattaa tcccatagat ttcatgtaaa acaacaatag
      661 ctacatcact tttatgtata tactttatca aattatccac cacctttaca aaaaaataaa
      721 tttttatatt taatttaatg tattcctcta aatatatcaa ttttctgttt taaaacttaa
      781 tttatttctt aattcaatat tatttactat gcattcctca aattaaaaag atatatatcc
      841 ttaatcagat acacgtctat aattatacta gttaatttac aaaattagct agacttttac
      901 aatcaattgc ttatcaaatt ggaattagtt aaatagaaaa aatcaaaatt gatgtaaaga
      961 cattatatag tacatgtagt aggtatgaac cccatataca attagttttt ctatatacta
     1021 aatcacacac aaagtgaaga agtataagac atactacata aaatccagat aaaattgtaa
     1081 ctgaaaagcc agatgctgcc catcttacag gatagtgtgt agatagaaaa agaaaagcat
     1141 ttatacatga tgtaattata ttgttttgta tcaatccagt taatctcgtt tgaatgtatc
     1201 ctctaaaaac aacttcttct gaaattgcag taacaataaa tgtaatagct gtagtaatag
     1261 taggaaataa aaaaacaact ttttttcctt ctataacttt attgaataaa ataatgaaaa
     1321 ttatgagtat agtagcacct aaaattccat aaaaaagact ttgctttaaa ttttttcttg
     1381 taattccaat tgtatttaat ttttcttttg aaaataatac aaaccccaac cctataaata
     1441 aaattaaggt tgaaaatatt ccttgcagca catttaaaat tgttggactt aaatttgttt
     1501 tatataaata gccttgtaaa aacaatacca acataattac taaataaaga aatatagctt
     1561 ttactccatc atttttagag tagttcttac ataatagctt atattcctta ttttcaagtt
     1621 caatattaaa cattaatgtt attacctcct agtcaaagtt atctttattt aacttttata
     1681 atgtatcata cttatactac atacgtcaaa ttttgactca tacattattc atattttgat
     1741 atatctactc tattcattag ttttgatgca aaagtatgca ttaacttttt tacgattatt
     1801 atacctattt cacatagtag aaaatctcta taaacccaat aatcacaatg tttttctatc
     1861 tactagtttt tgtttactgc ttcctagttc atctcaatta attttgtaaa aatctgttca
     1921 aatattagtc atttttagct aaaatttaat aactaaaaaa gagtacctta cttataattt
     1981 taaaaattat aaaataagat actcctaaaa tctactttat attaaattta ttttccaaaa
     2041 tatctattta ataatcctaa gaatgcttta ccatgtctag cttcatcttt acacatttca
     2101 tgtactgtat catgtatagc atctaatcct aattcttttg ctttcttagc taattttaat
     2161 tttccatcag ttgcaccata ctcagcatca actctaactc ttaagttttc ttttgtatct
     2221 gcaactacaa cttctccaag aagttctgca aatttagcag catgttcagc ttcttcaaga
     2281 gctattctct tataagcttc tcctacttct ggataacctt ctctatcagc ttgtctactc
     2341 attgctaaat acattcctac ttctgtacac tcaccaacaa agttagctct taatccttcg
     2401 attatttctt catctacacc ttgagctact cctattctgt gctcatcagc ccaatttttt
     2461 tcgcctttca tttcttcaaa tttatcagct ccaactttac atactggaca ttgtgctggt
     2521 gcagcatctc cttcatgtat atatccacat actgtacaaa caaatttctt cataattact
     2581 ttcctcctaa attttatata tcaaattaaa ttttttattt attaagttag tctatatata
     2641 cccgcaccat atacctttaa acaatttttt ttaaagtttt ttctaaatac ttattttccc
     2701 tgtgtcaata atatgtttct ataataatga ccttttaatt ttatgagttc tttatgattt
     2761 ccatattcca caattctacc attttccaaa actattatat tatcacaatt tgatatggta
     2821 cttaatctat gagctataat tatacttgtc tttccataag ttaaattatt tattgcttct
     2881 tgaattttag cttctgttgc tatatctata ttacttgtag cctcatccaa aataagtatt
     2941 ggagcatttt ttaatactgc tctagcaata cttattaatt gtttttgacc ttcacttaaa
     3001 tcttcacccc tatctgataa tacagtacta tatccttcac gcattttaac tataaattta
     3061 tgtgaatttg ctattttact agcatatata acttcttcat cagtagcctc cagatttcca
     3121 taccttatat tttccataac tgtatcattg aatatataac cttcttgtgg aactactcca
     3181 aaacaagttc tcaaatagtt tctgtcaata gaatatatat ccttaccatc taaaaatata
     3241 cttccttcat ctatatgata aaaatttgta attagattta taattgttgt ttttccactt
     3301 cccgtctctc ctatgatggc atttgatgtg ccagctttga tttttaggtt tatattgtct
     3361 aaaattttct tttttgaatc ataagaaaaa cttacatctt taagctcaat ttctcccttt
     3421 acaaaatgag agtttaaact cccatcttta ttatcgttaa gctcactttt aaacacgtta
     3481 tctacttctt gctcttcttg ttcatctata atttcaaaaa ctctttcaca tccagctatg
     3541 gctgaaagta atgtattaaa taaatttgcc aattcattta aaggtcttgt gaattgtctt
     3601 acataactta aaaaactagc tataacccct atggttataa tatctttaga tgcaagaacg
     3661 cctcctataa tacatatggt tgcaaatcct atattactaa tcacattcat aataggcatt
     3721 aaaagagaag tgtaaatttg tgatttaagg gaaatattta gaagtctatc attgacttct
     3781 ttaaaatcat ttacagattt tctttcataa ttaaaagatt ttactacatt tatgtttgat
     3841 atcatttctt caatatgtgc atttagtttt cccaactcaa cttgttgttg cttaaaaaaa
     3901 actctagttc tctttgcaat aaaattactc acaaacatta ctatcggtat taatattata
     3961 ctgcaaaagg ttaaaattgg gcttaataca aacatcataa taaaagtcat caatatgttt
     4021 aagattcctg taattaactg tactaatgaa tttgatattc ctgtactaat attatcaaca
     4081 tcatttgtta ctctgctcat aatatctcca gtagaacttt tatcaaaaaa ggttattggt
     4141 agatttttaa attttaaaaa taaattactt ctcatctctt taactatact ttgagataat
     4201 ctagctgtac atatcccctt agacagttta actgaaaact caattatata aaatgaaagt
     4261 aaaataatta ttccttttaa gaaaatggat tttgaataag tatcgatatc tattatgtca
     4321 actacctttc caatagtata tggtattaca gttgatataa ttgaatcaat tattactagc
     4381 aaaaaaataa atctagtaat cttcttatct tttttaaagt acataaaaat tcttttaaac
     4441 gtttttttaa aatctttagg tttttctttt ttctttaatc tactaacctt ttgatttcca
     4501 gtacttaaat tcaatatact tcctccttaa tttacatctt cacttactaa attctgcaac
     4561 gcaaaaattt ttttataaat tggagaacta accagtaaat cttcatgtgt agcaaatcca
     4621 accacctctc cctcatccaa gactaaaatc ttatcacagt caattataga actaatcttt
     4681 tgtgaaatag ttattacagt acatttctta ctttcctttt ctcttaaatt acttatatat
     4741 tcttttatat tctttcttat aattgtctct gtattaatat ccattgcact aaaacaatca
     4801 tcaagaatta atatttgtgg tttttttgct atagctctag ctatagtaat tctttgtctt
     4861 tgtcctcctg atagattctt acccctttga aataaaacct catcataagt attttcgagt
     4921 tttgttataa attgatttgc atttgctatc tcgcatgatt ttataacatc ctctttgcat
     4981 atatctctat ttcccataac tacattctcc aatatgcttt ttccaaacac actactattt
     5041 tgaaatacta cacctattct ttcccttaaa ctttcttcag aaatactgta tatactacgt
     5101 cctccaatct taatatcgcc tttatcaatt atatataatt tttgaattaa atttactaaa
     5161 gaagtctttc ctgaaccagt ttgaccaatc acaccaatat tttgaccttc ttctatttta
     5221 aaggatatgt tttttaatat tttttcatct gtatttttgt tatacgaaaa atctacattt
     5281 tcaaaaatta tatcacaatt tacaaacttt aaatcttttt ctccaacatc taaactttca
     5341 tctctctcaa ttacttcttc attctcatca accaaaacct cacttattct ctcataagac
     5401 gcttttgccc taacaaaaac attaaaaaca tgtgaaatca ttataagaga tgttaatatt
     5461 ctagtcatat agtttacata agcaactata atcccaattt taatttctcc tgaataaatt
     5521 aaatgccttc caatcagtaa tattattatt atagaaacat taatgattat tgtaattgtc
     5581 ggtctaaaaa atgacatcat agttgtagct tttctactat ttttaaaaag ttcatcattt
     5641 aactcttcaa aactttggtt ctcataatcg tatctacaaa atagtttaac tacttttatt
     5701 ccacttaaaa actgtcttag ttttgaattt actttgtctg tactttcttg tacattttta
     5761 aataatctat accctatctt catattgagc aatattataa taaatataat taaaactgca
     5821 attagaataa ttattgacat ctttaaattt aattttatag ctataattaa acttcctata
     5881 cacattattg gtgactttat aaaaactctc atcattccat taacgaattg ttgcatttga
     5941 ttgatatcat tagtaattct tgttattaat gaagctctcc caaatttatc tatttgtttt
     6001 aatgagtatg tatttatctt ttcaaataca tcctgtctta aatcgtaaga aaaatgctga
     6061 gaaactcttg ctgagattac acttcttatt attgcaaata ttgcacctaa aaaagttata
     6121 cctatcatta tagctcctgt tgatagtatg taatttaagt tcttatttga aacccctata
     6181 tctattattt ttgccattat agttggttga gctaaatcac aaaaagcttc caatgttaaa
     6241 aaaaatactg ctgttagaaa taatactata tattttttta tgtatttttt aaaatacctc
     6301 attcaatcat cccttcatag aattttctca ttaccaaaag tatatcataa tggctaattt
     6361 aattgattat atcaattata aatatttgat ttatta
//
LOCUS       DAEPXF010000197         6387 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain HB27
            SAMN13376389-rid12479423.denovo.204, whole genome shotgun sequence.
ACCESSION   DAEPXF010000197 DAEPXF010000000
VERSION     DAEPXF010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13376389
            Sequence Read Archive: SRR10518432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6387)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6387)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001069624.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001069624.1
            Genome Coverage       :: 315x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/15/2021 01:54:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,165
            CDSs (total)                      :: 4,076
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 89
            rRNAs                             :: 10, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 10 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 19 of 69
            Pseudo Genes (incomplete)         :: 44 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 10 of 69
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6387
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13376389-rid12479423.denovo.204"
                     /strain="HB27"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="China: Hebei"
                     /collection_date="2017"
     gene            205..648
                     /locus_tag="KRL67_003339"
     CDS             205..648
                     /locus_tag="KRL67_003339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HBH1524182.1"
                     /translation="MDEMSFKKELLELTRDINMKFTTLLSDFYQPLGITAVQALILSE
                     LCEHGEKKISDLGKNLNMTNSNVSVICQRLEKNGFLNRIRDIEDQRIVKVKVTNKSLD
                     IQEHISSSIFDSYFENMTSEKLQDMEDIIEGLEKLNKLLTYIDCK"
     gene            740..2557
                     /gene="ftsH"
                     /locus_tag="KRL67_003340"
     CDS             740..2557
                     /gene="ftsH"
                     /locus_tag="KRL67_003340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HBH1524183.1"
                     /translation="MDEHKPQKRSALYYYTVIIIVVMFLNALIFPSLMDKKMVEIDYG
                     KFLTMLDTGKVKSVEIENNRIAINPSSSKDKNIYITGRMDDPELVDRLKNAKVEFTKV
                     IPKENSPLLSILLTWIIPIGILMLFGNLMMKSMQKRMGGNAMQFGKSNAKMYVSAQSG
                     KRFNDVAGQDEAKEALTEIVDFLHNPEKYKKIGAQMPKGALLVGPPGTGKTLLAKAVA
                     GEANVPFFSISGSEFVEMFVGMGASRIRDLFKQAKEKAPCIVFIDEIDTIGKKRDNGS
                     GLGGNDEREQTLNQLLSEMDGFDGGIGVVILAATNRPDSLDKALLRPGRFDRRIPVEL
                     PDLGGREAILKVHAQNVNVSSDIDYNAIARSTSGASGAELANIVNEAALLAVKSGRDI
                     VEQQDFDESVETVIAGYQRKGAVISDKEKKIIAYHEIGHALVAAMQKHSAPVHKITII
                     PRTSGALGYTMQVAEDESVLMSKEEAIDKITTFTGGRAAEEVIFNTCTSGASNDIEQA
                     TKIARAMVTRLGMSEEFDMVALETVSNQYLGGDTSLACSSDTAAKIDEEVFKIVKACH
                     KNAIEILENNIDKLHELANYLLEKETITGDEFMEILNLN"
     gene            2735..5434
                     /locus_tag="KRL67_003341"
     CDS             2735..5434
                     /locus_tag="KRL67_003341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase KdpD"
                     /protein_id="HBH1524184.1"
                     /translation="MDRPNPDILLKKIKNEEEKLSRGQLKIFFGYAAGVGKTYSMLES
                     AQNLKKVGVDVVVGYIEPHTRPETLALLDGLEILPVKEIEYKSIKLKEFDLDAALARK
                     PEVILVDEFAHSNVAGLRHTKRWQDIEELLLAGINVYTTVNVQHLESLNDIVEIITNV
                     SVKETIPDKFLDTNTQLELIDVEPDVLLERFNEGKIYKKEQATRAKNNFFIKDNLVAL
                     REIALRKTAERVNKEVQMTRLSKGDVTVIPTSDTLLACISPSPSSAKVIRTASRISDS
                     TFAKWIALYVETPNTARLPFDEQKQLQNNLKLAKKLGGEIIVLHGENIIEQILRISKL
                     RNVTKIVIGRNHSSNKRFSKRFKKDMVDKLIDEVDYIDIHIIPYKFASDVKYKPKKDK
                     SSIKSKFKISKVDFIKLIFITLMISILAYAVQSMGFIRENILLIYMLGVVLVSMWTKG
                     YSTGIISSVFNIVLLNYFFTAPLYTLSIADSNYIVTLVVFSIVGIITSTLTSKIQHEA
                     ETAAKREENTKMIYQIIKGFLRLSNKDDIVNKGIELLSLSLSRDVICYLNVDKYNKSK
                     LYKKNTNNKDKNDLDSEDEKAVASWVLSNSTVAGNDTDTLPGSKGYYIPIVGMNLTLG
                     VVGISCIDSKLDTEDISLIETIIAQMAIALDREILSEAKENTNLEIERERLRSNLLRA
                     VSHDLRTPLAGISGAVSTIIKNKGTIGQDIIDELLSGVYEDAQWLIRLVENLLSMTKI
                     DEGKLEVKKHSELVEEIISEALQKIKKRIENALIDINIPEQILFVPMDAKLIEQVLIN
                     LIDNSLKYSKEDCKIEINVYEKDDYVWFEVSDNGPGISKELKKHIFDRFFTGEEGAKD
                     SRKGVGLGLSICKSIIQAHKGEIMVENNKDKGSTFKFSLPKEN"
     gene            5471..6169
                     /locus_tag="KRL67_003342"
     CDS             5471..6169
                     /locus_tag="KRL67_003342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBH1524185.1"
                     /translation="MSKKLILLVEDDKTIRKFISTALLTQDYDVKEAITGKEGISIAV
                     SYSPDVVLLDLGLEDMDGIEVIKAIRQFSNIPIIVVSAREQDRDKVEVFDAGADDYLT
                     KPFSIVELLARVRVAFRHSQVEAQQKDDVKSTFEVDKLLIDFDKRKVIVDDIEVHLTP
                     IEYNILSLLAKHHGKVLTHNFIIKEIWGSVIGNETKSLRVFMATLRRKIEKQPANPRY
                     IITEVGVGYRLNDE"
BASE COUNT         2529 a          718 c         1161 g         1979 t
ORIGIN      
        1 attaaatttt attaaaaata tcctgtataa aattaaatat caataagata acaaaattcc
       61 aattgaagtt acaatattgt aattgacaat aaatgcttaa tactataata tttaatacat
      121 taaacattat agtattaagt atttatttac agtatgcact tcagcctaaa gttaaaataa
      181 agtattttcg tagggagcgt ttgtatggat gagatgagtt ttaagaaaga gctactagag
      241 ttgactagag atataaatat gaaatttaca actttattaa gtgattttta tcagccactt
      301 ggtattacag cagtacaagc tttgatttta tctgagttat gtgaacatgg agagaaaaaa
      361 ataagcgatt tagggaaaaa cttaaacatg acaaatagta atgtatcagt aatctgtcaa
      421 aggcttgaaa aaaatggttt tttaaatcgc ataagagata tagaagacca acgaattgta
      481 aaggtaaaag ttacaaataa atcattggat atacaagagc atatttcaag tagtattttt
      541 gatagttatt ttgaaaatat gaccagtgaa aaattgcaag atatggaaga tataatagaa
      601 ggtttagaga aattaaataa acttttaact tacattgatt gcaaataaga taaaaataat
      661 ttaaaggaga ataaaattat ggatgaacat aaaccccgat tgcaaataag ataaaaataa
      721 tttaaaggag aataaaatta tggatgaaca taaaccccaa aaaaggtctg cactctatta
      781 ttatacagtt ataataatag tagtaatgtt tttaaatgct ttaatatttc caagtttaat
      841 ggataaaaaa atggttgaga ttgactatgg gaagtttctc acaatgttgg atacaggaaa
      901 ggttaaatct gttgagatag aaaacaatag aatagcaata aatccatcta gtagcaagga
      961 taagaatata tacataactg gtagaatgga tgacccagaa ttggtagata gactaaaaaa
     1021 tgcaaaagta gaatttacta aggttattcc taaggaaaat tcacctcttt taagcatttt
     1081 acttacatgg ataataccta taggtattct tatgctattt ggtaacttaa tgatgaagtc
     1141 tatgcaaaag cgaatgggtg gtaatgctat gcaatttgga aaaagtaatg ccaaaatgta
     1201 tgtatctgct caaagtggaa aaagatttaa tgacgtagct ggtcaagatg aggcaaaaga
     1261 agctttaact gaaatagtag atttcttaca caatccagaa aagtataaaa agataggagc
     1321 acaaatgcct aaaggtgcac ttttagtagg acctccaggt acaggaaaaa cactccttgc
     1381 aaaagctgta gcaggtgaag caaatgtacc atttttctca atatctggtt ctgaatttgt
     1441 tgagatgttt gtaggaatgg gagcttctag gattcgtgat ttatttaaac aagcaaaaga
     1501 aaaagctcca tgtatagtgt ttatagatga gattgatact attggtaaaa agcgtgataa
     1561 tggaagtgga ttaggtggaa atgatgaacg tgaacaaact ttaaatcagc tgctgagtga
     1621 gatggatggt tttgatggtg gaattggagt agtaatactt gctgctacaa atagacctga
     1681 cagtcttgac aaagcattac ttcgtccagg aagatttgat agacgtatac cagttgaact
     1741 tccagacctt ggaggacgtg aagctatatt aaaggtacat gctcaaaatg taaatgtaag
     1801 ctcagatata gattataatg caattgcaag gtctacatca ggagcatcag gtgcagaact
     1861 tgcaaatatt gtaaacgaag cagcgctttt agcagttaag tctggcagag atatagtaga
     1921 gcaacaggat tttgatgaat ctgttgagac tgtaattgct ggatatcaac gtaaaggagc
     1981 tgtaatttca gacaaggaaa agaaaattat agcatatcat gaaataggtc atgcacttgt
     2041 tgctgctatg caaaaacatt ctgcacctgt ccataagatt actataatac ctagaacttc
     2101 aggagcattg ggatatacta tgcaagttgc tgaagatgag agtgtactta tgtctaaaga
     2161 agaagcaatt gataaaataa ctacttttac aggtggtcgt gctgctgaag aggttatttt
     2221 taacacttgt acatcaggag catcaaatga tatagaacaa gctactaaaa ttgcaagagc
     2281 tatggttact cgccttggta tgagtgaaga gtttgatatg gtagctcttg aaactgtaag
     2341 caatcaatat ttaggaggag atacttctct tgcttgttct tccgacactg ctgctaaaat
     2401 agatgaagag gtatttaaaa tagtaaaggc ttgtcataaa aatgcaattg aaatcttaga
     2461 aaataatatt gataaattgc atgaacttgc taattattta ttggaaaaag aaactattac
     2521 aggtgatgag tttatggaaa tacttaactt aaactaaaat gattatacac aagataaaaa
     2581 tgcctattgt taggcatttt tattttataa ctaaattttt taaattcata tttaaatctt
     2641 tacataatct taatcactaa aaatacatct taactacaat tttatatcga aattgatatt
     2701 ataatagtaa tgaatggtgt aaggaggttt tttaatggac agacctaatc ctgatattct
     2761 attaaaaaaa attaaaaatg aagaagaaaa actaagtaga ggtcaattaa aaatattctt
     2821 tggttatgca gccggagtag gaaaaaccta ttctatgttg gaatctgcac aaaatctaaa
     2881 gaaagtgggt gtagatgtag tagttggata tattgaacca cacactagac cagaaacatt
     2941 ggctttactt gatggcttgg aaattcttcc tgtaaaggag atagaataca aatctataaa
     3001 attaaaagaa tttgatttag atgctgcttt agcaagaaaa ccagaagtta tattagttga
     3061 tgaatttgcc cattctaatg ttgctgggtt aaggcataca aaaagatggc aagatataga
     3121 agaattgctg ttagcaggca taaatgtcta cactactgta aatgtacaac atttagaaag
     3181 tctaaatgac attgttgaga taattacaaa cgtatcagtt aaagagacta taccagataa
     3241 gtttttggat acaaatacac agttagaact tatagatgtt gaacctgatg tacttcttga
     3301 aaggttcaat gaaggtaaga tatataagaa agaacaagct actagagcaa aaaataattt
     3361 ttttataaaa gacaacctag ttgcacttag agaaatcgca cttagaaaaa cagcagagag
     3421 agttaataaa gaagttcaaa tgaccagact atcaaagggt gatgtaactg taatacctac
     3481 atcagataca cttcttgcat gtataagtcc atctccatct tcagcaaaag ttattagaac
     3541 tgcatccaga atatcagaca gtacatttgc aaaatggata gctttatatg ttgaaactcc
     3601 aaacacagca agacttccat ttgatgaaca aaaacaacta caaaataacc taaaattagc
     3661 taagaaatta ggtggagaaa taattgtttt acatggtgaa aatataatag agcaaatact
     3721 tagaatttca aaactgagaa atgtaacaaa aattgtaata ggtagaaatc attcaagtaa
     3781 taaaagattt agtaaaagat ttaaaaaaga tatggttgac aaattaattg atgaagttga
     3841 ttatatagat atacatataa ttccatataa atttgcctca gatgttaaat acaaacctaa
     3901 aaaggataag tcatcaataa aatctaaatt taaaattagc aaagttgatt ttataaaact
     3961 tatatttatt actttgatga taagtatttt agcatatgca gtacaaagta tgggatttat
     4021 aagagaaaat atattgttaa tatatatgtt gggtgtagtt ttagtttcta tgtggactaa
     4081 agggtatagt acaggtataa taagttctgt atttaatata gttcttttaa attatttttt
     4141 cacagcacca ttatatacat tatctatagc agattcaaac tatatagtaa cactggttgt
     4201 attctcaata gtaggtataa taactagcac tcttacatct aagatacaac atgaagcaga
     4261 aactgctgct aaaagagaag aaaatacaaa aatgatatat caaataatta agggattttt
     4321 aagattatca aataaagatg atatagtaaa caaaggcata gaacttttgt ctcttagctt
     4381 aagtagagat gtaatttgtt atttaaatgt agataagtat aataaatcga aactttataa
     4441 aaaaaatact aataataagg ataaaaatga tttggatagt gaagatgaaa aagctgttgc
     4501 aagttgggtt ttatctaatt caacagtggc aggaaatgac acagatacac ttcctggttc
     4561 aaagggatac tatataccaa ttgtagggat gaatttaact ttaggagtag taggaatatc
     4621 atgtattgat tcaaaattgg atacagaaga tataagctta attgaaacaa taattgctca
     4681 aatggcaata gcgcttgata gagaaatctt gtcagaagct aaagaaaata ccaatctcga
     4741 gattgaaagg gaaagattaa gaagtaatct tttaagagca gtttcgcatg accttagaac
     4801 tccgctagca ggaatatctg gagcagtaag cacaattata aaaaataagg gtactatagg
     4861 tcaagatata atagatgaat tacttagtgg cgtatatgaa gatgctcaat ggttaataag
     4921 acttgttgaa aatttactta gtatgaccaa gatagatgaa gggaaattgg aagttaaaaa
     4981 acattctgaa ctggttgagg agattatatc agaagcgtta caaaaaataa aaaaaagaat
     5041 tgaaaatgca cttatagata ttaatatacc agaacagata ttatttgttc ctatggatgc
     5101 aaaattaata gaacaagttt taattaattt aatagataat tctttgaaat attcaaagga
     5161 agattgtaag atagagataa atgtatatga gaaagatgat tatgtttggt ttgaagtatc
     5221 tgataatggt cctggtatat caaaggaact taaaaagcat atctttgaca gattttttac
     5281 tggagaagaa ggagctaaag attccagaaa gggtgttgga cttggattat caatttgtaa
     5341 atctataatt caagctcata aaggtgaaat tatggtagaa aacaataaag ataagggttc
     5401 aacttttaag tttagtttgc ctaaggaaaa ttaatagttt taaattttaa aattagtttt
     5461 ggaggaagaa atgagtaaaa aacttatatt gttagtagag gatgataaga caataagaaa
     5521 atttatatct actgcacttt taacacagga ttatgatgtc aaggaggcta taactggaaa
     5581 agaaggtata tctattgctg tctcatattc tccagatgtt gttcttttag atttaggctt
     5641 agaagatatg gatggaatag aggtaataaa agctatcaga caattttcaa atataccaat
     5701 aattgtagtt tcagctagag aacaagatag agataaggta gaagtatttg atgcaggggc
     5761 agatgattac ttaacaaaac cattctccat agtagagtta ttagcaagag taagagtcgc
     5821 atttagacat tcacaagtag aagctcaaca aaaagatgat gttaaatcta catttgaagt
     5881 agataaatta ttgattgatt ttgataaaag aaaagttatt gttgatgata ttgaagtcca
     5941 tttgacaccc attgaatata atattctttc attacttgca aagcatcatg gaaaagtgct
     6001 tactcataat tttataataa aagaaatatg gggtagtgtt atagggaatg agactaagtc
     6061 tttaagggtc tttatggcaa cgttaagacg aaaaatagaa aaacaacctg caaacccaag
     6121 atatataatt acagaagttg gtgtaggata cagattaaat gatgaatagt tgcaatatat
     6181 taattattag attatgtgct tattaatata tattaaaatt atatagttta gttaaaataa
     6241 gaatgaagtg tatgtgctgt tgtcagttta gcacatgcat tttatttttt agcttttttt
     6301 agtatcaata attttttagt attaataata tgaaatatgt tttaaaataa gtatatagtg
     6361 taatctgtaa taaaaacttt ggttaaa
//
LOCUS       DAEPXF010000198         6381 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain HB27
            SAMN13376389-rid12479423.denovo.205, whole genome shotgun sequence.
ACCESSION   DAEPXF010000198 DAEPXF010000000
VERSION     DAEPXF010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13376389
            Sequence Read Archive: SRR10518432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6381)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6381)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001069624.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001069624.1
            Genome Coverage       :: 315x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/15/2021 01:54:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,165
            CDSs (total)                      :: 4,076
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 89
            rRNAs                             :: 10, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 10 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 19 of 69
            Pseudo Genes (incomplete)         :: 44 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 10 of 69
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6381
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13376389-rid12479423.denovo.205"
                     /strain="HB27"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="China: Hebei"
                     /collection_date="2017"
     gene            complement(109..906)
                     /locus_tag="KRL67_003343"
                     /pseudo
     CDS             complement(109..906)
                     /locus_tag="KRL67_003343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888187.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="M18 family aminopeptidase"
     gene            complement(923..3187)
                     /locus_tag="KRL67_003344"
     CDS             complement(923..3187)
                     /locus_tag="KRL67_003344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3553 domain-containing protein"
                     /protein_id="HBH1524186.1"
                     /translation="MNLDTLNPAQREAVEKTEGPVLILAGAGSGKTKVLTTRIAYLIE
                     DKGVQAPNILAITFTNKAANEMRERVEQNIGPETKDMWISTFHSCCVRILRKDINKIG
                     YNRSFVIYDSADQVTLVKDCLKELNLSDKVFEPKAVISAISGAKDKLYTPKQFKDINM
                     ADNRMVKIADIYALYQDRLKRNSALDFDDLILKTVELFKANDEVLAYYRSRFRYIMVD
                     EYQDTSKAQYELIKLLAREHQNICVVGDDDQSIYGWRGADIRNILEFEKDYDNVHVVK
                     LEQNYRSTQVILDAANKVISNNIERKRKKLWSEKKEGELIKIQLTGSEIEEADFIADS
                     IAQIARKENRPYKDFAVLYRANAQARPVEDALNRSQIPYNIYGGTKFYERKEIKDLLA
                     YLRVIQNPQDDISIKRIINVPRRGIGLRTIEKIEDRANLKQESIYSVLIDIETNSDIS
                     TKARASISGFVDIIGTLRTIKEVYPVSKLIEKVLDTTGYMDELVEIRNKNEKDLTGKG
                     EEAQDRIDNLREFISIALEFESSNDDTYENKDLETFLTSIALTSESNDEEDNDRVSLM
                     TIHTSKGLEFPVVFLTGMEEGLFPISRAIKSMSDSQIEEERRLCYVGITRAKEELYMS
                     LTEKRTLYGKTNVAIASRFMEELPEECIERLYKVKKELSYSKASYNMLDKYTKKYMST
                     ISKSKVADKVNATIKDSNKETNPDDIKLGSKVHHPKFGVGTVVSIIGTDVTIAFDQQG
                     IKKINKEYTTLNIL"
     gene            complement(3442..4260)
                     /locus_tag="KRL67_003345"
     CDS             complement(3442..4260)
                     /locus_tag="KRL67_003345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HBH1524187.1"
                     /translation="MFRINNLTYQYEKNTNALLNINMDFSKGNVIGIIGSNGSGKSTL
                     FMNLMGILKPTSGEILFKEEKLKYDKRSLYNLRKNVGIVFQDPEKQIFYSKVYDDIAF
                     AMRNIGMDEKTIKERINKALVAVNGIDFIDRPVHFLSYGQKKRVAIASVIAMENEIVL
                     LDEPTAGLDPVSTRSIVDIIKGLNKNNIKIVISSHDMNLMYEICDYIYVLDKGILIDE
                     GKAENVFINENNIIQAGLESPWLVKVHRNMNLPLFKKEEDLYKYWKERELNTNK"
     gene            complement(4253..4951)
                     /gene="cbiQ"
                     /locus_tag="KRL67_003346"
     CDS             complement(4253..4951)
                     /gene="cbiQ"
                     /locus_tag="KRL67_003346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt ECF transporter T component CbiQ"
                     /protein_id="HBH1524188.1"
                     /translation="MLIIDKYAYTNRLSKVNPNKKVAIGVIFLIASMVIQNIFILSGI
                     MILMSILVVCVAGIDLKNYLKLLRIPMYFLFLSIGITLINISFNKADLLYSFEFFSFN
                     VGISKASIDMSIHVLFRAMSCLTCVYFCILTTPFNQLIFFFKKLHLPDTFVELSMLIY
                     RFIFIFLEEFSEIYKSQELRFGYINLKTSYKSLGILGSMLYKRLMTRYDDMCISLDIK
                     LYDGKFHIVGDNDV"
     gene            complement(4954..5238)
                     /locus_tag="KRL67_003347"
     CDS             complement(4954..5238)
                     /locus_tag="KRL67_003347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     substrate-binding protein"
                     /protein_id="HBH1524189.1"
                     /translation="MSAKTKTKTNILLLLLVVALIIFPLLVNSGAEYGGADGQAESEI
                     TKINPNYKPWFSSPYEPPSGEIESLLFSAQAALGAGVIGYILGLQKGKRS"
     gene            complement(5228..5980)
                     /locus_tag="KRL67_003348"
     CDS             complement(5228..5980)
                     /locus_tag="KRL67_003348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006440549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter permease"
                     /protein_id="HBH1524190.1"
                     /translation="MKQNIKLGVIAALMLIILTPVTSNAMHIMEGYLPVKWSIAWGII
                     FIPFFLFGLKSIGKIVKQDPKKKVLLALCGAFVFVLSALKIPSVTGSCSHPTGVGLGA
                     IMFGPSVMFVLGTIVLIFQALLLAHGGITTLGANAFSMAIIGPIVSFLIFKVLKKKDG
                     NNAIPVFLAAAIGDLATYTITSIQLALAFPDPSGGVMASAIKFLGIFFMTQIPIAIAE
                     GILTVIVYNLITENGEKSILENNDKGVKANEC"
     regulatory      complement(6162..6354)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
BASE COUNT         2007 a         1154 c          701 g         2519 t
ORIGIN      
        1 agtattggat aggttctatc ctatctctac atataggttt ccctatatgc tgactatttt
       61 agctactaac tcatgactaa agtcacgagt gtgcgtagct attttttaat caaatacagc
      121 cattacattt acaccatgtt gacctttagt attaacaaga gcattcaaac tagcatgtac
      181 cattgcaaga ttatcttgtc tcccaataga tataaattca tcattagccc ctattaaact
      241 tcctttttca aattcatata aaaataaatc aaaatctata atatcttcca tatttacatc
      301 taagttagat gctatttgcc ttataagaaa attatctttt tctaatgtat catttaacag
      361 cccaactaaa ggtaacatat ctttttgctt attatatgaa tgaccatcat ttatgcttct
      421 atttaaatgt attgctagat ttggtattat acaaattggc tttttaaagt ctataagcct
      481 ttcatttggt tttaatatac tatcaccttt taaaactact ctaccagcta tcccaagagg
      541 tctatccaac catgtactta atattgctcc tccatagcat tctgtattta acttcaaata
      601 agatttttca gcttctattt ctgcatttgg tttaatacta aatgtaggag aatctgaatg
      661 agagcctatt attctaaatc catctctttc tatatcttct gaatttacta caaaagctac
      721 tatagctgat aaattcttag taacataata ctttccacca acccttagat tccatctctg
      781 attcaaaact aattcttcaa atccattttt atctaagatt tccttagtac tctcgactgc
      841 atgaaaagac gacggactat catatatgaa gtctattaag tttctagcaa aatttatact
      901 atccattcct catttacctc cattataaaa tatttagtgt tgtatattct ttatttattt
      961 ttttaattcc ctgttggtca aaagctatag taacatctgt accaattata gaaactactg
     1021 tcccaacccc aaatttagga tgatgtactt tagaaccaag ttttatatca tctggattag
     1081 tttctttatt agaatctttt attgtagcat taactttatc tgcaacttta gatttactta
     1141 tagtactcat atacttttta gtatatttat ctaacatatt ataacttgct ttagaatagc
     1201 ttagttcttt ttttactttg tacaatcttt ctatacattc ttctggcaat tcttccataa
     1261 atcttgaagc tattgcaaca tttgttttac catataatgt tctcttctct gtcaatgaca
     1321 tatatagctc ttctttagct ctagttatac caacataaca aagtcttctc tcttcttcta
     1381 tttgtgaatc actcatagat tttatagctc ttgatatagg aaataatcct tcttccatac
     1441 ctgttaaaaa cacaacaggg aactctaaac ctttagatgt atgaattgtc ataagcgaaa
     1501 ccctatcatt gtcttcttca tcatttgatt cagatgtaag tgctatactt gttaggaatg
     1561 tctctaagtc tttattttca taagtatcat cattactact ttcaaactcc aaagctatag
     1621 atataaattc tctcaaatta tcaattctgt cttgagcttc ttcaccttta cctgttaaat
     1681 ctttttcatt tttatttctt atctctacaa gctcgtccat atatcctgta gtgtccaaga
     1741 ctttttctat aagtttacta acaggataaa cttcttttat agttctaaga gtccctataa
     1801 tatcaacaaa tccacttata cttgcccttg ctttagtaga tatatctgaa tttgtttcta
     1861 tatcaattaa tactgagtat atactttcct gttttaaatt agctctatct tcaatttttt
     1921 ctatagttct aagccctata cctcttctag gaacatttat aatccttttt atagatatat
     1981 catcttgtgg attttgaata actcttaaat acgccaacaa atcctttatt tcttttctct
     2041 catagaattt tgtaccacca tatatattgt atggaatttg acttctattt aaagcgtcct
     2101 ctacaggtct tgcctgtgca tttgctctat aaagtactgc aaaatcctta taaggtctat
     2161 tttcttttct agctatttgt gcaattgaat cagcaatgaa atcagcctct tctatttcgc
     2221 taccagttaa ttgtatcttt ataagttctc cttctttttt ttcactccaa agcttttttc
     2281 tttttctttc tatattatta gatataactt tattagctgc atctaatatc acttgtgtag
     2341 acctataatt ttgctctaat tttacaacat gtacattgtc ataatctttc tcaaattcaa
     2401 gtatgtttct tatatctgcg cctctccatc cataaatact ttggtcatca tctccaacaa
     2461 cacaaatatt ttgatgttct cttgctaata attttatcaa ttcatattga gctttacttg
     2521 tatcttggta ctcatctacc attatgtatc taaatctact tctgtaatat gctaacactt
     2581 catcattagc tttgaaaagt tctacagttt taagtattaa atcatcaaag tcaagtgcac
     2641 tatttctttt taaacggtct tgatataacg cataaatatc tgcaatctta accattctat
     2701 tatctgccat attgatatct ttaaactgct ttggagtata aagtttgtct tttgctcctg
     2761 aaattgcact tataactgct tttggttcaa acactttatc acttaaattt aattctttta
     2821 gacagtcttt aaccaatgta acttggtctg ctgaatcata aattacaaaa cttctattgt
     2881 aaccaatctt atttatatct tttcttagta tacgaacaca gcaagaatgg aatgtactta
     2941 tccacatatc cttagtttca ggacctatat tttgttcaac tctttctctc atttcatttg
     3001 cagctttgtt tgtaaatgtt atagctaata tatttggtgc ttgtacacct ttatcttcta
     3061 taagatatgc tattcttgtt gttaatacct tagttttacc tgaaccagca ccagccagta
     3121 taagtactgg accttcagtc ttttctactg cttctctttg agcaggattt aatgtatcta
     3181 aattcatatt tctcctcctg ttaacagcca taaatatatt ttaatctaaa atatgaatta
     3241 cttcaaatta taattatgta ttatattaat agattatttc caatatatta caattaaata
     3301 acatttttcc atataattta tttcattgtt taataaaaaa aataacctta tgatttgggg
     3361 aatcataagg cacctttaag gggtctaggt taagggttat tatatataaa tctatttttt
     3421 taaaataggt ttagggctta attatttatt tgtattaagt tctctttcct tccagtactt
     3481 atacaaatct tcttctttct taaataatgg taaattcata tttctatgta cttttactaa
     3541 ccatggggat tccaggccag cttgtataat attattttca tttataaaca cattttctgc
     3601 ctttccttca tcaatcaaaa tccccttatc caatacatat atgtaatcac atatttcata
     3661 cattaaattc atatcatgac tggatattac tatttttata ttatttttat ttaatccctt
     3721 tattatatct acaatagacc tagtacttac cgggtctaaa ccagctgtag gttcatcaag
     3781 taatacaatt tcattttcca ttgctataac tgatgctatt gccactcttt ttttctgtcc
     3841 ataactaaga aaatgtactg gcctgtctat aaaatctatt ccattaacag ctactagagc
     3901 tttgtttatt ctctctttaa ttgttttttc atccatacca atgtttctca ttgcaaaggc
     3961 tatatcatca tatacttttg aataaaaaat ttgtttttct gggtcttgga acacaatacc
     4021 tacatttttt ctaaggttat ataaacttct cttatcatat tttaactttt cttctttaaa
     4081 aagaatttct ccagatgtag gctttaatat acccattaaa ttcataaata atgttgattt
     4141 tcctgaacca tttgaaccta ttatgcctat aacatttccc tttgaaaaat ccatattaat
     4201 gtttaatagt gcattggtat ttttttcata ttgatacgtt aaattattta ttctaaacat
     4261 cattatcccc tactatgtga aatttaccat catacaattt tatgtctaat gatatacaca
     4321 tatcatcata ccttgtcata agtcttttgt acagcatact tccaagaata cctaaggatt
     4381 tatatgaagt ttttaaattt atatacccaa atcttagctc ttgtgacttg tatatttctg
     4441 aaaattcttc taaaaatatg aatataaacc tatatattaa cattgataac tcgacaaaag
     4501 tatcaggtaa atgaagtttt ttaaaaaaga atattaattg attaaagggt gttgtaagta
     4561 tacaaaaata cacacaagtc aaacaagaca tagctctaaa taacacatga atagacatat
     4621 caatagacgc ctttgatatt cctacattaa aactaaaaaa ttcaaaacta tataataaat
     4681 ctgccttatt aaaagatata tttataagag ttattcctat actaagaaat aaaaagtaca
     4741 ttggtattct aagtaatttt aaataatttt ttaaatctat tccagccaca caaacaacca
     4801 aaatactcat taaaatcata attccactta aaataaatat attttgtatt accatagaag
     4861 caatcagaaa aattactcct atggctactt ttttgtttgg atttacttta cttaatctat
     4921 ttgtatatgc atatttatca attataagca tgattaactt ctctttcctt tttgaagacc
     4981 taatatgtat cctattacac cagcccctaa agctgcttgt gcagagaata ataaactttc
     5041 tatttctcca cttggtggct catatggact gctaaaccat ggtttgtaat ttggatttat
     5101 cttagtaatt tcactttcag cttgaccatc tgctcctcca tattcagcac ctgagttgac
     5161 aagtaatggg aatataataa gagctactac taataaaagt aataatatgt tagtttttgt
     5221 ttttgtttta gcactcatta gctttaactc ctttatcatt attttctagt atgctttttt
     5281 ctccgttttc tgtaattaaa ttatatacaa ttacagtaag aataccctct gctattgcta
     5341 ttggtatttg agtcataaag aaaattccta aaaatttaat tgctgaagcc attaccccac
     5401 ctgatgggtc tgggaaagcc agtgctaatt gtattgaagt tattgtatat gtggctaagt
     5461 ctcctattgc tgctgcaaga aatactggaa tagcattatt tccatctttt ttctttaaaa
     5521 ccttaaatat taggaatgat acgattggcc ctattatcgc cattgaaaaa gcattagctc
     5581 ctaaagtagt tattcctcca tgtgctaata gtaatgcttg gaatattaaa actatagttc
     5641 caaggacaaa cattacgctt ggtccaaaca ttattgctcc aagacctaca ccagtgggat
     5701 gtgaacaact tcctgttact gaaggtattt ttagtgctga taatacaaac acaaatgctc
     5761 cacacaaggc caatagtact ttctttttag ggtcttgctt tactatttta cctattgatt
     5821 ttaatccgaa taagaaaaat ggtataaata taattcccca tgctatagac cattttactg
     5881 gtaagtaacc ttccattatg tgcattgcat ttgatgtaac tggagttaaa atgattaaca
     5941 taagtgcagc tataacccct aatttaatat tctgtttcat cttagtatcc ctctctccct
     6001 ttctaataat taaaacataa taatacatgt ctgatttatt taataaaata aaaaagccta
     6061 actcgatggt taggcttcta attttccaca atctaaatac aaacacataa gacaaacata
     6121 tctgtataat tattataaga aactttctcc atccccgaag aaattacttt aatattttag
     6181 gcaggtctcc tgacttagct tcttcctact cctcttcctt cccatgagat ataaaaaata
     6241 tcatcatagt ggctaacaaa agtttcgtct gcatcacagt agcgggggct gtaaaggaat
     6301 ttaacctttt tccctattaa tcttgcttga agaacctata atattatata tttaattttt
     6361 aaattattct taaattattt t
//
LOCUS       DAEPXF010000199         6356 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain HB27
            SAMN13376389-rid12479423.denovo.206, whole genome shotgun sequence.
ACCESSION   DAEPXF010000199 DAEPXF010000000
VERSION     DAEPXF010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13376389
            Sequence Read Archive: SRR10518432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6356)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6356)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001069624.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001069624.1
            Genome Coverage       :: 315x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/15/2021 01:54:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,165
            CDSs (total)                      :: 4,076
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 89
            rRNAs                             :: 10, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 10 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 19 of 69
            Pseudo Genes (incomplete)         :: 44 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 10 of 69
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6356
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13376389-rid12479423.denovo.206"
                     /strain="HB27"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="China: Hebei"
                     /collection_date="2017"
     gene            <1..157
                     /locus_tag="KRL67_003349"
     CDS             <1..157
                     /locus_tag="KRL67_003349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HBH1524191.1"
                     /translation="VEENSTNELKLSMSIGYAIFDEKVDVSLFDTYRRADSQMYDNKR
                     KINKQYQ"
     gene            complement(238..1956)
                     /locus_tag="KRL67_003350"
     CDS             complement(238..1956)
                     /locus_tag="KRL67_003350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative 2-aminoethylphosphonate ABC transporter
                     permease subunit"
                     /protein_id="HBH1524192.1"
                     /translation="MNNNAISSKSISLKSKMNLDIKKCMLTVLVMSLVIFLLAPIIYL
                     FVQAFLDKDGVFVGLQNFQEYLASESLLNSLKNTIFVSVISTVISLALALVYAYALTR
                     TNIKFKGFFKSLAMFPMFAPTMMHAIGLVYLFGNQGLITNLLGVHVPIYGAVGIILAQ
                     IIYVFPQSFQILYISLSSTDYRLYEASNTMGISKIREFISITIPSIKYSLISSAFVSF
                     TLCFTDFGAPKVIGGEFNVLSIDVYKQIIGQQNMPMGATVSILLLIPAIISFIVTKKI
                     EKNQNSYITSKSIPYKVKTNKLRDSILGGIVGIIAFGIIAVMLTVILSSLISVWPYDL
                     RLTLEHFSMKNSIDGIKPFINSIKMSLLTAILGTSFVFLFAYLNEKTEKAPYLKSFGY
                     FLSTLPMALPGLVLGIAYVLFFNKLEFNFMDTIYIKNLFNGLYGTLLIMVICNIVHFF
                     SVAFITATTAIKKVDKELDMVAKSMGINSASILKNVTIPLCLPAVLENFMYFFLNSMV
                     TISALVFIYTASSKVAAISIVQLDDKGSTAQAAALAVLILVTNILVKVIYEFIKNRLE
                     KRTNFN"
     gene            complement(1961..2842)
                     /locus_tag="KRL67_003351"
                     /pseudo
     CDS             complement(1961..2842)
                     /locus_tag="KRL67_003351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728969.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
     regulatory      3118..3213
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            3268..3444
                     /locus_tag="KRL67_003352"
     CDS             3268..3444
                     /locus_tag="KRL67_003352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH1524193.1"
                     /translation="MFKNKMDKCTHVLTAYISSSHDYCNFLDTQLDDFILEYGENVVE
                     SCLHQVMILLSKYN"
     gene            complement(3681..3830)
                     /locus_tag="KRL67_003353"
     CDS             complement(3681..3830)
                     /locus_tag="KRL67_003353"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH1524194.1"
                     /translation="MEIVAVYYIAKFLLDLYKVKSHSKSEAKSGFIFNLTINKLIVKI
                     KYKKY"
     gene            4083..4271
                     /locus_tag="KRL67_003354"
     CDS             4083..4271
                     /locus_tag="KRL67_003354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH1524195.1"
                     /translation="MTKTILCDYCNKGINKDDNKYITFHKKSHMKTNICINCALNLID
                     KDKLNENIINNQHGYSKK"
     gene            complement(4520..5224)
                     /locus_tag="KRL67_003355"
     CDS             complement(4520..5224)
                     /locus_tag="KRL67_003355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH1524196.1"
                     /translation="MRLKFKSKESTSREKHQSAESLAKFMLNNKSFLGITLSILDEWK
                     NNIDSSKIEDIVKNNFRFEESIIKDKNYKEFIKLLNSFYNGTEDELNGRRGRFLELIW
                     DVVGTYNGKEFTQKIDEAIVLENEVKISEKDIDIVYIGEIKDKGIANFIEMHECKASA
                     NTTLRTPLKPKHRGKLELMQSVIEISETEEIKCDGLIITFNANKRRLERKLKSYGFEY
                     FKIIPRAEIEERVFNK"
     gene            complement(5226..5657)
                     /locus_tag="KRL67_003356"
     CDS             complement(5226..5657)
                     /locus_tag="KRL67_003356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-export chaperone SecB"
                     /protein_id="HBH1524197.1"
                     /translation="MELDKMKYRKLVNDVELVNVLIKNIELSDVILHKDENIKNESIK
                     CDVNMKYECEEFKKLSEVVEFYPKFNLRIECQGEGLVNLKFELRAIYKFNSIEDYEDD
                     YILMFIERNVPVNIWPYAREIISSITTRIGYPALVISPYKA"
     gene            complement(5675..6238)
                     /locus_tag="KRL67_003357"
     CDS             complement(5675..6238)
                     /locus_tag="KRL67_003357"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH1524198.1"
                     /translation="MNNYSIMFENKSILDLKQTCYLDSLYNLFEEKIQYVTKTLKNNN
                     IEEDEWDIQFIKEDALQSIKNIEEDFITDKTLDWRINLSKIIKKVLDKSKANFKIADE
                     MYHNPMKKSEQYYDLLSNIFDNLEELICIKYKEYEISRNIRFTNIGNLVNNDNSSNYI
                     DNEHIEFDFNFKFEIEDYDNDDYALAA"
BASE COUNT         2166 a          948 c          699 g         2543 t
ORIGIN      
        1 agttgaagaa aactcaacta atgaattaaa attatcaatg tctattggat atgctatttt
       61 tgatgaaaaa gtagatgttt ctttatttga tacatatcgt cgtgcagatt ctcaaatgta
      121 tgataataaa aggaaaatta ataaacaata tcaataatac atttaaaaag tctattcttt
      181 aaatagtaaa tcataagaat agacttttta aatttgtgtt tttataagat aaattattta
      241 attaaagttt gttctttttt ctaaacgatt ctttataaat tcatatatta ctttcactaa
      301 tatatttgtg actaatatta acacagctag tgctgctgcc tgtgcagtac tccccttatc
      361 atctagttgt acaattgata ttgcagctac ttttgaactt gcagtatata taaaaactag
      421 tgctgatatt gttaccatag agtttaggaa aaagtacata aagttctcta aaactgctgg
      481 caaacaaagt ggtatagtta cgttcttaag aattgatgcg ctatttattc ccattgattt
      541 agctaccata tccaattctt tatctacttt cttaatagca gtagtagctg ttataaatgc
      601 tactgaaaag aaatgaacta tattacatat aaccattata agtaatgttc catataatcc
      661 attaaacaaa ttcttaatgt atattgtatc cataaaatta aactctaatt tattaaaaaa
      721 taatacataa gctataccta aaactaatcc tggcaatgcc ataggcaatg tacttaaaaa
      781 atacccaaat gattttaaat atggggcttt ttctgttttt tcatttaaat atgcaaataa
      841 gaaaacaaag ctcgtcccta aaatagctgt caatagtgac atttttattg aatttataaa
      901 tggtttaatt ccatcaatac tgtttttcat agaaaagtgt tccagtgtaa gtcttaaatc
      961 atatggccaa actgatatta atgatgataa gattacagtt aacattacag ctattatacc
     1021 aaatgcaata attcctacaa tccctcctaa aatactatct cttaacttat tagttttaac
     1081 cttgtatgga attgactttg aagttatata tgaattttga tttttttcaa tttttttagt
     1141 tactataaac gatattattg caggtattaa aagtaatatt gatacagttg ctcccattgg
     1201 catattctgt tgccctataa tttgcttata tacatctatt gataatacat taaactctcc
     1261 tcctatcacc tttggagctc caaaatctgt aaaacataat gtaaatgata caaatgcaga
     1321 acttattaaa gagtatttta tacttgggat tgttatgctt ataaattctc ttatcttgct
     1381 tattcccata gtattactag cttcatatag tctataatcg gttgaactta gagatatata
     1441 aagtatttga aatgattgag gaaatacata tataatttgt gctaatatta ttcccactgc
     1501 tccatatatt ggaacatgca ctccaagtaa attagttatc agcccttgat ttccaaataa
     1561 atacactaga cctattgcat gcatcattgt tggagcaaac ataggaaaca ttgctaatga
     1621 tttaaaaaaa cctttaaatt taatatttgt tctagtcaat gcatatgcat atactaatgc
     1681 aagtgctagt gatattacag ttgatataac tgaaacaaat attgtatttt ttagagaatt
     1741 taatagagat tcagaagcca agtattcttg aaagttttgt aatcctacaa agactccatc
     1801 tttatctaaa aaagcctgta caaatagata tattataggt gccaataaaa atattactaa
     1861 agacataact aatactgtaa gcatacattt ttttatatct aaattcattt ttgatttaag
     1921 acttatactt ttagaactta tcgcattatt attcatacgc ttatgctcct ttaacttttg
     1981 gatactttaa atagtatttt tcgtctatac ttatttttac aaggtcacct tctactaaat
     2041 ttaaattaac ccactctttt atagaaacat cacttattat gaaattttca tttagtttat
     2101 cttttatttc aactcttaat aaattccctc taaactctat atttgatatt atcccttggt
     2161 aattttcttt tgtcgctttc tctatttgaa catattctgg tcttacagtt aatatagaat
     2221 ctccattatc aaaacagttt gtatctccta taaattgagc tgtaaataga ttttgaggat
     2281 tttggtatat ttcttctgga gttcctattt gcattatatc tcctccattt agaatagcta
     2341 ttttatctgc catagttata gcttcttctt ggtcatgcgt taccattatt gtcgttatat
     2401 taagttcttt ttgtaatctt ttaatatcca ttctaagctt gtgtctaacc tttgcatcta
     2461 atgcggacat tggttcatct aaaagtaaaa actttggttc aagtgctaat gctcttgcta
     2521 ttgctatcct ctgttgttgc ccacctgata atgctttagg atatttatga gcttcattag
     2581 tcaatccaac tgtttcaagt acttccttaa ctttattatc tattttttct tttgaaactt
     2641 ttctctcttt tagtggaaaa gctatattgt tataagcagt catattcgga aataatgcat
     2701 atgattgaaa tactatacca aaacctctct ttgatggctc taaatttgta atatctttat
     2761 cctggagaat tatagctcca ctatttacat cttcaagacc tgctattatt ctaagtaatg
     2821 tagttttacc acatccactt ggctattatt agttagtcca aataaataaa atacttataa
     2881 caattgaaat acgcgatata cactgaacct cccatgacta aagtcacagg gttcctgttt
     2941 catagaattt cgcataatca aatgttagtt cacaatacta ttctaactta ccttgacctt
     3001 taaatactct gttccttttt aggaataaat actattcata cctataaatt tatttaattt
     3061 tcaaacaacc ttgttgaaca ttaactaatt ctattgtatt attttagttg tataacaaaa
     3121 ctaaatcggc aaaactagag aaaattagtg acgcaaagct atagggacta agatttataa
     3181 aattcttata aattatgcca gccagttgcc aaaaagatat tgttcttttt gtttttatga
     3241 aagtttttta gggggataat aatttatatg tttaaaaata aaatggataa atgtacacat
     3301 gtactgactg cttatattag cagttcacat gattattgta attttttaga tacacagtta
     3361 gatgatttta tactagaata cggagaaaat gtagtggaat cttgtttaca tcaagtaatg
     3421 atattgttaa gtaagtataa ttagatagac attgatttct agagaaccct ttttagtttt
     3481 atctactttt aaataatgat atgataataa aaaattaatt aaaatagatt tataatattc
     3541 atagttttat tttttcttaa aatgcgatat aataaagatg taaattcgta tcaaaaagag
     3601 ataagagaac tgcaattctc ctacctcttt agaaataagg aactataatc tattgtcgag
     3661 agtgtagttt ccgaaaaaat ttagtacttt ttatatttaa tttttactat tagtttatta
     3721 atagttaaat taaatataaa gccactctta gcttccgact ttgagtggct ttttactttg
     3781 tataaatcaa gtaaaaattt tgctatgtaa taaactgcta ctatttccac acttcccacc
     3841 tcctttcatt agaaagtagg ttagagcata atgtggaaac tgccactctt agatatttag
     3901 ttccttacat ataaattata acataatttt acaattatca aatatccatt ctccatattt
     3961 ttttatttat acaatatatt ctatattgca ataaaaaaca ttgttttaaa tatatataca
     4021 taggtatata ctaaatatgt taaacttaat tatggaaaat ataaaatgaa aggaataaaa
     4081 atatgactaa gactatatta tgtgattact gtaataaagg aataaataaa gatgataata
     4141 agtatattac ttttcataag aaaagtcata tgaaaactaa catttgtatt aattgtgcat
     4201 taaatttgat agataaagat aaattaaatg aaaatattat aaataatcaa catggttatt
     4261 caaagaaatg aaaaagcact ctccacaacg aagaatgctc tatataataa tgtttgactc
     4321 agtaaatatg cgttggggtt acatatttac ttttttatta tatcattaat tttgtgcatg
     4381 aaaaagagtt atttaaatca attttaaggt gtgttgagta aggttcttga tagtttatat
     4441 gttaatgaat ttcaaaaaaa tagcactctt acaaaaagag tacttttagt atctattaat
     4501 ttgtataaat tagtgtaaat tacttattaa acactctttc ctctatttct gctctaggaa
     4561 ttattttaaa gtattcaaat ccatagcttt ttagctttct ttcaagcctt cttttgttag
     4621 cattgaatgt aattattaat ccatcacatt taatttcttc tgtttctgat atttcaatta
     4681 ctgattgcat cagttctagt tttcctctat gttttggttt taatggagtt cttagtgttg
     4741 tgtttgctga tgctttacat tcgtgcattt caataaaatt tgctatccct ttatctttaa
     4801 tttcgcctat atagacaata tctatatctt tttctgatat tttcacttca ttttctaaca
     4861 ctatggcctc atctattttt tgtgtaaatt cttttccgtt atatgttcct acaacatccc
     4921 aaatcaactc taaaaatctt cctcttctac cgtttaactc atcttctgtt ccgttataaa
     4981 atgagtttaa taattttata aactctttat agttcttatc tttaattata gattcctcaa
     5041 atctaaaatt attttttaca atatcctcaa ttttactact atcaatatta tttttccatt
     5101 catctaatat tgacagtgtt atacctaaga acgatttatt gtttaacata aactttgcta
     5161 gagattctgc tgactgatgt ttttccctgc tagtactctc tttagattta aatttcaatc
     5221 tcatattatg ccttatatgg agaaattact agagcaggat atccaatacg agttgtgatg
     5281 gaacttatta tctctcttgc ataaggccat atattaactg gaacatttcg ttctataaac
     5341 attaatatat aatcatcttc ataatcctct attgaattaa atttataaat tgcccttagt
     5401 tcaaatttta aatttaccaa accttctcct tgacattcta ttcttaagtt aaatttagga
     5461 taaaattcta ctacttctga taatttttta aattcttcac actcatattt catattaaca
     5521 tcacatttaa tactttcatt tttgatattt tcatctttat gtaatataac atcacttaat
     5581 tcaatatttt taattagcac atttactagt tccacatcat taactaattt tctatacttc
     5641 attttatcta attccattaa tcttcctcca aaatctaagc tgccaatgca taatcatcat
     5701 tatcataatc ctctatttca aacttaaagt taaaatcaaa ttcaatatgt tcattgtcta
     5761 tataatttga tgagttatca ttgttaacta aatttccaat attagtaaac ctgatatttc
     5821 tagaaatttc gtattcttta tattttatac atataagctc ttctaaatta tcaaagatat
     5881 tacttaataa atcataatac tgttcacttt tcttcatagg attatgatac atttcatctg
     5941 ctattttaaa gttagccttg cttttatcta atactttctt tattattttt gataaattga
     6001 ttctccaatc taatgtttta tcagtaataa aatcttcttc tatattttta attgactgca
     6061 aggcatcttc ttttataaac tgtatatccc attcatcctc ttctatattg ttatttttta
     6121 gagttttagt tacatactga attttttctt caaataaatt atataagcta tctaaatagc
     6181 atgtttgttt taaatctaaa atagacttat tttcaaacat aattgaataa ttattcatat
     6241 taaattcccc cttgtaaaca aatctttcca taaatatacc tccaagagta taatttaaga
     6301 tttgttacct aaattatacc attttaatat ttacattcca acaacaaata ttatta
//
LOCUS       DAEPXF010000200         6330 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain HB27
            SAMN13376389-rid12479423.denovo.207, whole genome shotgun sequence.
ACCESSION   DAEPXF010000200 DAEPXF010000000
VERSION     DAEPXF010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13376389
            Sequence Read Archive: SRR10518432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6330)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6330)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001069624.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001069624.1
            Genome Coverage       :: 315x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/15/2021 01:54:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,165
            CDSs (total)                      :: 4,076
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 89
            rRNAs                             :: 10, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 10 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 19 of 69
            Pseudo Genes (incomplete)         :: 44 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 10 of 69
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6330
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13376389-rid12479423.denovo.207"
                     /strain="HB27"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="China: Hebei"
                     /collection_date="2017"
     gene            complement(73..1218)
                     /locus_tag="KRL67_003358"
     CDS             complement(73..1218)
                     /locus_tag="KRL67_003358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine reductase"
                     /protein_id="HBH1524199.1"
                     /translation="MSKKVIADVFLEVANAIESGEFGKKVKIGVTTLGSEHGVENMVN
                     GAQLAKSNLFDIVLIGPKVETDLEVVEVNDEKEMHAKMEELLDSGYIDACVTMHYNFP
                     IGVSTVGRVITPAKGKEMILATTTGTSATNRIEAMVRNAIYGIATAKSMGNKCPKVGI
                     LNVDGARQVEKCLKELKDNGYDMEFADSIRADGGCVMRGNDLLVGAPDVMVTDTLSGN
                     IFMKVFSSYTTGGDYEAQGFGYGPGVGEDYDRKVLIVSRASGSPVVANALKYAYDVVK
                     GDISNVARNEFAKVKKAKFDDIISSLTKKEVKAEKVEVKMPDKEIVTRQIAGVDIMDL
                     EDAVSELWKNGIYAESGMGCTGPIVLVNEAKGDLAVETLVKAGYTAK"
     gene            complement(1229..2761)
                     /locus_tag="KRL67_003359"
     CDS             complement(1229..2761)
                     /locus_tag="KRL67_003359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014520415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III family protein"
                     /protein_id="HBH1524200.1"
                     /translation="MTYPVLKGAGYVLIHTPDMIVQNGSTCTVERATNPDSEFLKEVS
                     NHIRSYEDVVNYMPNQVYIGNRRPEELRDLPMPWCEQKIEGTRNGKFGEIMPQDEFIA
                     LMQISDAFDLVKLSQEFIDEVKPKIENNYPEIAPFVGKLKGDDIEEGKELVATHIAEG
                     LYHDGKFVGYVKRAHDVDVNLNAHTMFENLVVKASGVLSAIQMLRHSKIDPAEIDYVI
                     ECSEEACGDINQRGGGNFAKSIAEIAGLQNATGSDTRGFCAAPTHALIQAAALVKAGI
                     HKNVMVVAGGASAKLGMNAKDHVKKGLPVLEDVVGGFAVLVSENDGVNPVIRTDLTGK
                     HTVGTGSSPQAVMTALITSGLDRANLKITDVDVYSVEMQNPDITKPAGAGDVPEANYK
                     MIGALAVKRGDLEKKELKDFVSNKGLPGWAPTQGHIPSGAPYIGFLIDDLTTGNRNRA
                     MIVGKGSLFLGRMTNLFDGVSFIAERNTGVTEETSGISKDEIKKIIAESMKKLALDML
                     EE"
     gene            complement(3018..4328)
                     /gene="grdB"
                     /locus_tag="KRL67_003360"
     CDS             complement(3018..4328)
                     /gene="grdB"
                     /locus_tag="KRL67_003360"
                     /EC_number="1.21.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:complement(3279..3281),aa:Sec)
                     /transl_table=11
                     /product="glycine reductase complex selenoprotein B"
                     /protein_id="HBH1524201.1"
                     /translation="MGKLKAVHYINQFFAGIGGEEKADTKPHVAETLPPISLQLDKLL
                     GEDIEIVGTVVCGDSYFNENIDSASEEVLSMVKGFEPQLFIAGPAFNAGRYGVAAGTI
                     TKVVKDALNIPALTGMYVENPGADMFKKDVYVVETSDSAAGMRKALPKIAKLAVKLAN
                     GEEIGTPKDEGYIARGIRVNYFHEDRGSKRAVDMLVKKIKGEPFETEYPMPNFDRVDP
                     SKAVKDLSKCKIALVTSGGIVPKGNPDRIESSSASKYGTYSIAGVMDLTEETYETAHG
                     GYDPVYANLDADRVLPVDVLRDLEKEGVIGKLHETFYTTVGNGTSVANSKKYASEIGA
                     ALVADGVDAVILTSTUGTCTRCGATMVKEIEKTGLPVVHMCTVVPISLTVGANRIVPT
                     IAIPHPLGNPALDPTEEKALRRGLVEKALNALTTEVDGQTVFEK"
     gene            complement(4360..4833)
                     /locus_tag="KRL67_003361"
     CDS             complement(4360..4833)
                     /locus_tag="KRL67_003361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011986236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:complement(4699..4701),aa:Sec)
                     /transl_table=11
                     /product="glycine/sarcosine/betaine reductase complex
                     selenoprotein A"
                     /protein_id="HBH1524202.1"
                     /translation="MSLLSNKKVLIIGDRDGIPGPAIEECVKTVEGAEVVFSSTECFV
                     UTAAGAMDLENQNRVKDAADKFGAENVVILLGAAEAEAAGLAAETVTAGDPTFAGPLA
                     GVALGLSVYHVVEEPIKSLFDESVYEDQISMMEMVLEVEEIEEEMSGIREEFCKF"
     gene            complement(4945..6231)
                     /locus_tag="KRL67_003362"
     CDS             complement(4945..6231)
                     /locus_tag="KRL67_003362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006439082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine/sarcosine/betaine reductase component B
                     subunit"
                     /protein_id="HBH1524203.1"
                     /translation="MRLELGKIFIKDIQFGDVTEVKDGVLFINKQEMLQEIGGDEHIK
                     SIDIELARPGESVRITPVKDVIEPRVKVEGNGGIFPGIMSKVDTVGEGKTHALKGVAV
                     VTTGKIVGFQEGIIDMTGEGAKYTPFSKLNNVVVIAEPIDGLKQYAHEKAVRMIGFKA
                     AMYLGEVARNLTPDEVSVYETKPLLESIKEYPELPKVGYVYMLQTQGLLHDTYVYGVD
                     AKQIVPTLLYPTELMDGAIVSGNCVSACDKNPSYVHINNGVVEDLYARHGKDINFVGV
                     IITNENVYLADKERSSNWTSKLCKYLGLDAVIVSQEGFGNPDTDLIMNCKKIEMQGVK
                     TVIVTDEYAGRDGGSQSLADADVRADAVVTGGNANQVVVLPKLDKVIGHLEVVDVIAG
                     GSDGSLRADGTIEVEIQAITGATNETGFGHLTAKGY"
BASE COUNT         1816 a         1325 c          767 g         2422 t
ORIGIN      
        1 aataaaaaaa acatataatt ataatcaata agattataac tatatgttct tatttacata
       61 taaaaaatgt acttattttg cagtatatcc agctttaact aaagtttcaa ctgctaaatc
      121 tcctttagct tcatttacta aaactattgg acctgtacaa cccataccac tttcagcata
      181 aattccgttt ttccataact cagatactgc atcttctaaa tccattatat caacaccagc
      241 tatttgacga gtaacaatct ctttatctgg cattttaact tctacttttt cagctttaac
      301 ttctttttta gtaagagaag atataatgtc atcaaatttt gcttttttaa cctttgcaaa
      361 ttcatttctt gcaacattac ttatatctcc ctttacaaca tcataagcgt attttaaagc
      421 atttgctact accggagaac ctgatgctct tgatactata agtacctttc tatcatagtc
      481 ctctccaact ccaggtccgt atccaaagcc ttgagcttca taatctccac ctgtagtata
      541 tgaagaaaat actttcataa atatatttcc agataatgta tcagtaacca taacatcagg
      601 tgcgcctaca agtaaatcat tacctctcat tacacatcca ccatctgctc taattgaatc
      661 cgcaaattcc atgtcatatc cattatcttt taattctttt aagcacttct caacttgtct
      721 tgctccatca acattaagta ttccaacttt tgggcatttg ttacccattg atttagctgt
      781 agcaatacca taaatggcat ttctcaccat tgcctctatt ctattagtag cgcttgttcc
      841 tgttgttgtt gcaagtatca tttcttttcc ctttgctgga gttattactc tacctactgt
      901 agatacacct attggaaaat tatagtgcat agtaacacaa gcatcaatat atcctgaatc
      961 taataattct tccatttttg catgcatttc tttttcatca ttaacttcaa ctacttcaag
     1021 atcagtttcc acttttggtc caataagcac aatatcaaat aaattagatt ttgctaattg
     1081 tgcaccatta accatgttct ctacaccatg ttcacttcca agtgtagtaa caccaatttt
     1141 tacttttttg ccaaattcgc cgctttctat tgcattagct acttctaaga atacatctgc
     1201 gattactttt tttgacatat cctcacccct attcttctaa catgtctaat gctagttttt
     1261 tcatagattc agctattatc ttcttaattt catccttaga tattcctgaa gtctcttcag
     1321 taacacctgt atttctttca gctataaaag atactccatc aaataagtta gtcattcttc
     1381 ctaagaataa actacctttt ccaactatca tagctctatt tctatttcct gtagttaaat
     1441 catctattaa aaatcctata taaggagctc ctgatggtat atgtccttga gttggtgccc
     1501 atcctggtaa tcctttgtta gaaacaaaat ctttcaattc tttcttttct aagtctccac
     1561 gtttaacagc aagtgctcct atcattttgt agtttgcttc tggaacatct cctgctcctg
     1621 ctggcttagt tatatctggg ttttgcattt caactgaata cacatcaaca tctgttattt
     1681 ttaaattagc tctatcaaga cctgaagtta taagtgctgt cataactgct tgtggtgaag
     1741 aaccagttcc aacagtatgc ttacctgtta aatcagttct tataactgga tttacaccat
     1801 cattttcaga tacaagtact gcaaatcctc caacaacatc ttcaagtaca ggaagacctt
     1861 ttttaacatg gtctttagca ttcattccaa gtttagcact tgctccacct gctactacca
     1921 ttacattttt atgtattcca gcttttacta aagctgctgc ctgaattaat gcatgagttg
     1981 gtgctgcaca aaatcctctt gtgtcagaac cagttgcatt ttgaagtcct gctatctcag
     2041 ctattgattt agcaaagttt cctccacctc tttggtttat atctccacaa gcttcttctg
     2101 aacactcaat aacataatct atttcagcag ggtctatttt gctatgtctt agcatttgaa
     2161 tagcagataa aactccagat gcttttacta ctagattttc aaacattgta tgggcattta
     2221 agttaacatc tacatcatgt gctcttttta cataaccaac gaattttcca tcatggtata
     2281 atccttcagc tatgtgtgtt gctactaatt ctttaccttc ttctatatca tcacccttta
     2341 atttacctac aaatggtgca atttcagggt agttattttc gatttttggc tttacttcat
     2401 caataaattc ttgtgataat tttactaaat caaatgcatc acttatttgc attaaagcta
     2461 tgaactcatc ttgaggcatt atttctccaa acttaccatt tctagttccc tcaatctttt
     2521 gttcacacca tggcattggt aaatctctta attcttctgg tcttctattt cctatataaa
     2581 cttgatttgg catgtagtta actacatctt cataacttct tatatgatta cttacttctt
     2641 ttaaaaattc tgaatctgga tttgttgctc tttcaactgt acaagtactt ccattttgca
     2701 ctatcatatc tggagtatga ataagtacat agcctgcccc ttttaaaact ggataagtca
     2761 ttttgtgcac ctccataaat tttatgaaca cttatttttt aatcttatta aacttattaa
     2821 aagttctaat cattgtattt tattgtatat ttgattctat tattgtttat gtattttggc
     2881 attttttaag atttcatgaa tgaataactc caatttatcc attcatgaaa tctatattat
     2941 tttttttgtg tatagttatt ttttattttt agtgtgtgtt tatattttta tctatataat
     3001 atatgtgtgt gttattttta tttttcaaat actgtttgac catctacttc tgtagttaaa
     3061 gcatttaatg ctttttctac aaggcctctt cttagggctt tttcttctgt agggtcaagc
     3121 gctggatttc caagtggatg aggtattgct atagttggaa ctattctatt agcccctact
     3181 gttaatgaaa taggtactac tgtacacata tgaactactg gaagtccagt tttttctatt
     3241 tcttttacca tcgttgcacc gcaacgagta caagtacctc atgtagaagt taatattact
     3301 gcatctacac catcagccac taatgctgct ccaatttcag aagcatattt tttagagttt
     3361 gcaacagatg taccattacc tacagttgta tagaaagtct catgtaattt tccaattaca
     3421 ccttcttttt ctaaatctct aagaacatca acaggtaata ctctatctgc atctaaattt
     3481 gcatacactg ggtcatatcc accatgtgca gtttcataag tctcttctgt taaatccata
     3541 actcctgcta tagaataagt tccatattta gaagctgaag aagactctat tctatctgga
     3601 tttccttttg gaactatacc accagaagta actaaagcta ttttacattt tgataaatct
     3661 tttacagctt tacttgggtc aactctatca aagtttggca ttgggtattc agtttcaaat
     3721 ggttcacctt ttattttctt aactaacatg tcaacagctc tcttagaacc tctgtcttca
     3781 tggaagtagt taactcttat tcctctagct atgtatcctt catctttagg agttccaatt
     3841 tcttctccat tagcaagttt tactgcaagt ttagctatct taggaagtgc ttttctcata
     3901 cctgctgcag aatcagaagt ttctactaca taaacatcct ttttgaacat gtctgctcct
     3961 ggattttcaa catacatacc tgttaaagct ggtatattta aagcatcttt tacaacttta
     4021 gttattgttc cagcagcaac tccatatcta ccagcattaa acgcaggacc agctatgaaa
     4081 agttgtggtt caaaaccttt taccattgat aaaacttctt cacttgcact atcaatattt
     4141 tcattgaaat agctatctcc acaaactact gttccaacta tttcaatatc ttcacctaat
     4201 aatttatcaa gttgtaaact tattggaggt aaagtttctg ccacatgtgg cttagtatca
     4261 gctttttctt ctccacctat accagcaaag aactgattaa tataatgtac tgcttttagt
     4321 tttcccatct cttagtttca ccccttttgc ttttaggcat taaaatttac aaaattcttc
     4381 tcttatacca gacatttctt cttctatttc ttcaacttct aaaaccattt ccatcatact
     4441 tatttggtct tcatatacac tttcatcaaa taatgatttt attggttcct caacaacgtg
     4501 gtaaacactt aatcctaagg caactccagc aagtggtcca gcgaaagttg gatctccagc
     4561 agttactgtt tcggctgcaa gacctgcagc ttcggcttca gcagcaccta gtaaaatcac
     4621 aacattttca gctccgaatt tatcagcagc atctttaact ctgttttgat tttctaagtc
     4681 catagcccca gcagctgttc agacaaagca ttctgtagat gagaaaacaa cctctgctcc
     4741 ttctactgtt tttacacatt cttctatcgc aggtcctggt ataccatcac ggtcacctat
     4801 tataagaacc tttttattac taagtaaact cataataatc ctccttatta tatttctaat
     4861 atatttttat atatctataa cacttattat aattatctat aatatgtttt attaacatta
     4921 ctcaatcgtt taataaaata cttcttaata tcctttagca gttaaatgac caaatcctgt
     4981 ttcattagtt gcacctgtta tagcttggat ttcaacttca atagttccat cagctcttaa
     5041 acttccatca gaaccaccag ctattacatc tactacttct aagtgtccaa taactttatc
     5101 taacttagga agaactacaa cttggttggc atttccacct gttacaacag catcagctct
     5161 tacatctgca tctgcaagtg attgagaacc tccatcacgt ccagcatact catctgtaac
     5221 tataactgtt ttaacacctt gcatttctat cttcttacag ttcattataa ggtcagtatc
     5281 aggatttcca aaaccttctt gtgaaactat aactgcatct aatcctaaat atttacataa
     5341 tttagaagtc cagtttgaag atctttcttt atctgctagg taaacatttt cattagttat
     5401 aattacacct acaaagttta tatctttacc atgtcttgca tataaatctt caacaacccc
     5461 attgtttata tgaacataac ttgggttttt gtcacatgca gaaacacaat taccacttac
     5521 aattgctccg tccattaatt ctgttggata taataaagtt ggtactattt gttttgcatc
     5581 tactccatat acataagtat catgtaataa accttgagtc tgaagcatgt aaacatatcc
     5641 tacttttgga agctctggat attcttttat tgattcaaga agaggtttag tttcatatac
     5701 tgaaacttcg tctggagtaa gatttcttgc tacttctcct aagtacattg ctgctttgaa
     5761 tcctatcatt ctaacagctt tttcatgagc gtattgtttt aaaccatcaa taggttcagc
     5821 tattactact acattattta atttagaaaa tggagtatac ttagctcctt ctccagtcat
     5881 gtctattata ccttcttgga aacctactat tttaccagta gttacaacag ctacaccttt
     5941 taaagcatga gtttttcctt ctcctacagt gtcaacttta gacatgatac cagggaagat
     6001 tcccccatta ccttcaactt ttactcttgg ttcaataaca tcttttacag gtgttattct
     6061 tacgctttca cctggacgag ctaattctat atctatagat ttaatatgct catctccacc
     6121 aatttcttgt agcatctctt gcttattgat gaataatact ccatctttta cttctgttac
     6181 atcaccaaat tgaatatctt ttataaagat tttccctaat tcaagacgca tacttgcacc
     6241 tcctgttatt taatttgtgt atatgcattt gagtttttct caaaattata tcattttaca
     6301 atttgtctta tttttttaaa gatatattat
//
LOCUS       DAEPXF010000201         6319 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain HB27
            SAMN13376389-rid12479423.denovo.208, whole genome shotgun sequence.
ACCESSION   DAEPXF010000201 DAEPXF010000000
VERSION     DAEPXF010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13376389
            Sequence Read Archive: SRR10518432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6319)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6319)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001069624.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001069624.1
            Genome Coverage       :: 315x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/15/2021 01:54:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,165
            CDSs (total)                      :: 4,076
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 89
            rRNAs                             :: 10, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 10 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 19 of 69
            Pseudo Genes (incomplete)         :: 44 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 10 of 69
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6319
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13376389-rid12479423.denovo.208"
                     /strain="HB27"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="China: Hebei"
                     /collection_date="2017"
     gene            complement(<1..668)
                     /locus_tag="KRL67_003363"
     CDS             complement(<1..668)
                     /locus_tag="KRL67_003363"
                     /EC_number="3.5.4.19"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoribosyl-AMP
                     cyclohydrolase/phosphoribosyl-ATP diphosphatase HisIE"
                     /protein_id="HBH1524204.1"
                     /translation="MDNKCNNVYSDEAEEFIRSIKFDDKGLVPVVVQEVVSKDVLMLA
                     YMNKDAIKKTLKDKVACYFSRSRQELWVKGETSGNTQKVVKMSYDCDVDTILLFVEQT
                     GVACHTGNYSCFYRDLFDDSSKMELEVQTNILKELYDLINERKNNPVEGSYTNYLFEK
                     GIDKILKKVGEESSEVIIASKNTDKSELIYEISDLVYHTLVLMIEKGVEIDEIKKELL
                     KRRK"
     gene            complement(696..1454)
                     /gene="hisF"
                     /locus_tag="KRL67_003364"
     CDS             complement(696..1454)
                     /gene="hisF"
                     /locus_tag="KRL67_003364"
                     /EC_number="4.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000880088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HBH1524205.1"
                     /translation="MLTRRIIPCLDVRNGRVVKGKKFKDIVDVDSPEVLGKFYSDSGA
                     DELVFYDITASNEDRKTSLEFVTKVAENINIPFCVGGGVNKLEDFTDILRKGADKVSI
                     NSSAVKNPELIREASLKFGAQCVVLSIDAKKNEEDSWSVYVKGGREKTNLDAIEWAVK
                     GVELGAGEIVVNSMDEDGMKNGYDIELLSKITSLVNVPVIASGGAGKMEDFYEAVNKS
                     NVDGILAASVFHFGEIKINDLKKYLKDMGVEVRL"
     gene            complement(1436..2158)
                     /gene="hisA"
                     /locus_tag="KRL67_003365"
     CDS             complement(1436..2158)
                     /gene="hisA"
                     /locus_tag="KRL67_003365"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HBH1524206.1"
                     /translation="MIIFPAIDIKDNKCVRLTQGEFDKVNVYYDNPLEVAYKWKNEGA
                     EYIHIVDLNGARSEFGVNTKIIEDIANNIDIPIQVGGGVRDKEKVKSLINAGVTRVIL
                     GSIAVENLNLVEELVNEYKEKIVVSIDAKDGKVAVRGWEVVSNVDSLTLCKQLEKIGV
                     QTIVYTDISKDGMLQGPNFEIYEKIAKETSLNVIASGGVTSIEDIKRLKAMNLYGAII
                     GKALYDKKIDFKEAQQLCLLEE"
     gene            complement(2155..2760)
                     /gene="hisH"
                     /locus_tag="KRL67_003366"
     CDS             complement(2155..2760)
                     /gene="hisH"
                     /locus_tag="KRL67_003366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HBH1524207.1"
                     /translation="MNIIVDYGLGNIDSVSRGFRKAGIETKISSDINEIKQADSLILP
                     GVGAFRDSISALDKLGLIPVIKEHVSKGKFIIGICLGMQLLYEKSYEYGEYEGLGLIK
                     GSIDKLDISLKVPHMGWNNLKFNKANDDILKYINEDDYVYFVHSYYANSSNEELVAFS
                     EYEKKIPAIVRKGNVYGIQFHPEKSGEVGLNILRAYGEMIK"
     gene            complement(2762..3340)
                     /gene="hisB"
                     /locus_tag="KRL67_003367"
     CDS             complement(2762..3340)
                     /gene="hisB"
                     /locus_tag="KRL67_003367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazoleglycerol-phosphate dehydratase HisB"
                     /protein_id="HBH1524208.1"
                     /translation="MRTWKVERNTLETQILVELNIDGSGKAEIDTGIGFLDHMLTLMS
                     FHGKFDLKVICKGDTYVDDHHSVEDIGIAIGEAFKNALGDKKGVRRYSNIYIPMDESL
                     SMVAIDISNRPYLVFNAKFDTQMIGSMSTQCFKEFFRAFVNESRVTLHINLLYGENDH
                     HKIESIFKAFARALKEGSEIVSNEIASSKGVL"
     gene            complement(3343..4392)
                     /gene="hisC"
                     /locus_tag="KRL67_003368"
     CDS             complement(3343..4392)
                     /gene="hisC"
                     /locus_tag="KRL67_003368"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HBH1524209.1"
                     /translation="MREKESIRELRGYEPNHVNCKVKLDANEGSKRLFKYLIKEISDS
                     DIDLNLYPEDSYSNLKESIIDYINISGVNKKNLLVGNGSSEIIDLIIHTFVDKDEVIL
                     SFSPSFSMYSIYSQINGSKFIGVESDENLVINIDSVIEKVKENNPKIVIVCNPNNPTG
                     TILKREEIIKLLDSTNSLVVLDEAYMDFGEESMLSDVFKYDNLIVLRTLSKAFGLAGI
                     RTGYMLSNSSLINSVEKVRPPYNLNSLSDFIATRALRNKDVVKAYIKEVKEEREVLYK
                     EMIGMGIKAYKSQANFILFYSEIENLSQKLIDRGVLIRKFGGKLENYYRVTIGDKEEN
                     SMFVGAIRDILKKEK"
     gene            complement(4426..5052)
                     /locus_tag="KRL67_003369"
     CDS             complement(4426..5052)
                     /locus_tag="KRL67_003369"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HBH1524210.1"
                     /translation="MDYLTIALAKGRIEGESFKKFKKMGLGDSIDTDTRKLIFKDEEN
                     KIIYIHVKPSDVVTYVEKGVADLGIAGKDTILENETDVYEIYDLGFGKCKFAVAGLKG
                     DSIYREDEYLKVATKYPNIAKKYFKEKGQKIEIIKLNGSVELAPIVGLSDVIVDIVET
                     GNTLKANGLEILEDICNISARIISNRASYRFKYEQIQNIIRLFEELDN"
     gene            complement(5074..6018)
                     /locus_tag="KRL67_003370"
     CDS             complement(5074..6018)
                     /locus_tag="KRL67_003370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase regulatory
                     subunit"
                     /protein_id="HBH1524211.1"
                     /translation="MKFLRIEDEVIYFKKRYKLKREIDKLFLDEEYFQIEPQLFEEYD
                     EFTTINNKIPEESMVKVVNGKVMVLRADITTSIIKSLVPRWEDGLKLKLFYNSSIYKN
                     KNTVGIKEIRQIGCEYLGEASVEADREVVKLALKILEKYNNNFILEVGSSKYIHGLLE
                     ELNLNKNCENQIKNLLYTKNTHELKVYIEELKIKREVKELLSNILSLQGNLYNVIEKS
                     NKFYCNNKMKQALEELKQVNNLIEECNFLDKARFDLSMITMLDYYEGIMFRGYYPNSY
                     KEILSGGRYDSLTKEYGKEIPAIGFTLSVDELMKYVYK"
BASE COUNT         1952 a         1243 c          536 g         2588 t
ORIGIN      
        1 tattttcttc ttttaagtaa ttcttttttt atttcatcaa tttcaacacc tttttctatc
       61 attaaaacca atgtatgata tactaaatcg ctaatttcgt aaataagttc acttttatcg
      121 gtattttttg atgctataat tacctcacta ctttcttcac ctactttttt aagaatctta
      181 tctattcctt tttcaaaaag ataattagta taagaacctt caactggatt attttttctt
      241 tcatttatta aatcatataa ttctttcaat atatttgttt gtacttcaag ctccattttt
      301 gaactatcat caaacaagtc tctgtaaaaa caagaataat tgcctgtatg acaagctaca
      361 ccagtttgtt caacaaaaag tagtatagta tctacatcac aatcataaga catcttaact
      421 actttttgag tattaccaga tgtctcacct ttaacccaaa gttcttgtct acttctacta
      481 aaatagcaag ctactttatc ttttaaggtt ttctttatag catctttatt catatatgct
      541 aacatcagta catctttact aactacctct tgaacaacaa cagggactaa ccctttatca
      601 tcaaacttta tacttcttat aaattcctct gcttcatcac tgtatacatt gttacattta
      661 ttatccatat ttctaatctc cttttagtat ttaacttata atcttacttc tacacccatg
      721 tcttttaaat atttctttaa atcgtttatt tttatctcgc caaaatgaaa aacagatgct
      781 gctaaaattc catctacatt agacttatta accgcctcat aaaaatcttc catttttcca
      841 gcgcctccag atgctatgac aggtacattt actaaagaag ttatctttga taataattct
      901 atatcatatc catttttcat tccatcttca tccatactat ttacaacgat ttctcctgct
      961 ccaagttcta cacctttaac agcccattca attgcatcca gattagtttt ttctcttcca
     1021 ccttttacgt atacgctcca tgaatcttct tcatttttct ttgcatctat agataaaaca
     1081 acacattgtg ctccaaattt taatgaagct tctcttatta attcaggatt tttaactgct
     1141 gatgaattta tagaaacttt atcagcacct tttcttaaaa tatcagtaaa atcttctaat
     1201 ttatttactc ctccaccaac acaaaatggt atatttatat tttctgcaac ttttgtcaca
     1261 aattctaagg atgtctttct atcttcattt gatgcagtta tatcatagaa tacaagttca
     1321 tctgcaccac tatcactata aaattttcca agtacttctg gactatcaac atctactata
     1381 tctttgaatt tttttccttt tacaaccctt ccatttctta catctaaaca aggtattatt
     1441 cttctagtaa gcataattgt tgtgcctcct taaagtctat ctttttatca taaagtgcct
     1501 tacctattat tgcaccatat aagttcatag cttttagtct ttttatgtct tctatagaag
     1561 ttactcctcc agaagcaata acgtttaatg acgtttcttt tgctattttt tcatatatct
     1621 caaaatttgg accttgtaac atcccatctt ttgagatatc tgtataaaca atagtttgta
     1681 cacctatctt ttcaagttgt ttacacaatg ttaaggagtc tacattactt acaacttccc
     1741 aacctctaac tgctaccttc ccatccttag catctataga aactactatt ttctctttat
     1801 attcattgac caattcttca accaaattta aattttcaac tgctatactc cctaatatta
     1861 ctctagtaac accagcattt attaaacttt tgactttttc cttatctcta actccaccgc
     1921 caacttgaat aggtatatct atattgtttg ctatgtcctc aattattttt gtatttacac
     1981 caaattcact tctagcacca tttaaatcta ctatatgtat atattctgct ccttcatttt
     2041 tccatttata tgctacttct aatggattat cataatatac atttactttg tcaaattctc
     2101 cttgtgtgag tcttacacac ttattatctt ttatatctat tgctggaaat attatcattt
     2161 tatcatctcc ccatatgctc ttaatatatt taaccctact tctccacttt tttcaggatg
     2221 aaattgtata ccatatacat tgccttttct aacaatagca ggtattttct tttcatattc
     2281 tgaaaatgca actagttcct catttgatga atttgcataa tatgagtgaa caaagtatac
     2341 atagtcatct tcatttatgt atttaagtat atcatcattt gccttattaa atttcagatt
     2401 attccatccc atatgtggca cttttaaact tatatccagt ttatcaatac ttccttttat
     2461 aagccctaat ccttcatatt ctccatactc ataacttttt tcataaagca attgcattcc
     2521 tagacatata cctattatga atttcccctt agaaacatgt tcttttatta ctggtatcaa
     2581 acctagttta tctaaagcac ttatagaatc tctaaaagct ccaacacctg gaagtataag
     2641 tgaatctgcc tgttttattt catttatatc actagaaatt ttagtttcta ttccagcttt
     2701 tctaaaaccc ctagacacag aatctatatt tccaagacca tagtctacta ttatattcat
     2761 gctacaaaac cccctttgaa gatgctatct cattagaaac tatttcacta ccctctttta
     2821 aagctcttgc aaatgcttta aaaatacttt ctatcttatg atggtcattt tctccatata
     2881 agagattgat atgtaatgtt actctagatt catttacaaa agctctaaaa aattctttaa
     2941 agcattgagt actcatgcta ccaatcattt gggtatcaaa tttagcatta aatacaagat
     3001 aaggtctatt gcttatatct attgctacca ttgatagaga ttcatccata ggtatataga
     3061 tattagaata tcttcttact ccttttttgt cacctagagc atttttaaat gcttcaccta
     3121 tagctatacc tatatcttct acactatggt ggtcatctac atatgtatca cccttacaaa
     3181 tcacttttaa atcgaacttg ccgtgaaatg acattaaggt aagcatatgg tctaaaaatc
     3241 ctattccagt atctatttca gctttaccag aaccatcaat atttaattct actaaaatct
     3301 gtgtttcaag agtatttctt tctaccttcc aagttctcat acctatttct cctttttcaa
     3361 aatatctctt atagctccaa caaacataga attttcttct ttatcaccaa ttgtaactct
     3421 gtaataattt tctaactttc caccaaattt tcttattaaa actcctctat caataagttt
     3481 ttgtgataaa ttttcgattt cagaataaaa taatataaaa tttgcttgtg atttatatgc
     3541 tttaattccc ataccaatca tttctttata taaaacttct ctttcctctt ttacttcctt
     3601 tatataagcc tttaccacat ctttatttct aagtgctctt gtagcaataa agtctgataa
     3661 tgaatttaaa ttatatggag gtcttacttt ctcaacagaa tttattaaac tactattaga
     3721 taacatatat cctgttctta ttccagccaa tccaaatgcc tttgataaag ttctaagaac
     3781 tattagatta tcatacttaa aaacatcact taacatgctt tcttctccaa aatccatata
     3841 tgcttcatct aaaaccacta gactattagt tgaatctaaa agctttatta tctcttccct
     3901 ctttaatatt gttccagttg gattattagg attacataca attactattt taggattatt
     3961 ctcctttact ttttcaatca cactatctat atttataact aaattttcat cactttcaac
     4021 acctataaat ttagacccat ttatctgact atatatagaa tacatactaa aacttggaga
     4081 aaaactcaat attacttcat ctttatctac aaaagtatgt attattaaat caattatttc
     4141 acttgaaccg tttcctacta gaagattttt cttatttact cctgaaatat ttatgtaatc
     4201 tattatagat tctttcaaat tactataaga atcttcagga tataaattta aatctatatc
     4261 actatctgaa atttctttaa taagatattt aaacaatctc ttacttcctt catttgcatc
     4321 aagttttact ttacagttta cgtgatttgg ttcatatccc cttaactctc ttatgctttc
     4381 tttttctctc aacttaaatc cctccttata aaactcaatt atattttaat tatcaagttc
     4441 ttcaaataaa cgaattatat tctgaatttg ttcatattta aatctatagc tagctctatt
     4501 tgatataatt ctagcactta tattgcatat gtcttctaat atttcaagac catttgcttt
     4561 aagtgtattg ccagtctcaa ctatatctac aattacatct gacaacccta ctattggagc
     4621 tagttctaca gagccattta attttattat ttcaattttt tgacctttct ctttaaaata
     4681 cttttttgct atattgggat actttgttgc cacttttaga tattcatctt ctctatatat
     4741 gctatctccc tttaatcctg ctacagcaaa tttacatttt ccaaaaccta aatcatatat
     4801 ttcatataca tctgtctcat tctctaaaat agtatcttta cctgctatac caaggtcagc
     4861 gactcctttt tcaacatatg taactacatc tgatggcttt acatgtatat atataatttt
     4921 attttcttca tctttaaaaa ttagttttct cgtatctgtg tctatactat ctcctagacc
     4981 cattttttta aattttttaa atgattcccc ttcaattcgt ccttttgcta gtgctatagt
     5041 caaataatcc aaagcaatcc ctccttctaa actttactta tatacatact tcattaattc
     5101 atcaacactt agtgtaaatc caatagctgg aatttctttt ccatattctt tagttaaaga
     5161 atcatatctt ccaccactaa gaatttcttt atatgagttt gggtagtatc ctctaaacat
     5221 aattccttca taatagtcaa gcatagtaat cattgataaa tcaaatctag ccttatctaa
     5281 aaagttacat tcctcaatta aattatttac ttgctttaat tcttcaagag cttgcttcat
     5341 cttattatta caatagaact tgtttgattt ttcaataacg ttgtataaat tcccttgcaa
     5401 gcttaaaata tttgataata gctcttttac ttcacgctta attttcagtt cttcaatata
     5461 tacttttaat tcatgagtgt tcttcgtata tagtaaattt ttaatttgat tttcacaatt
     5521 cttatttaaa tttaattctt ctaacaaacc atgaatatac ttgctactcc caacctctaa
     5581 aataaaatta ttattgtatt tttcaagtat cttaagagct aatttcacta cttctctatc
     5641 tgcttctaca gaggcttctc ctagatactc gcaacctatt tgtcttatct cttttattcc
     5701 aactgtattt ttatttttat atattgatga attataaaaa agcttaagct ttaaaccatc
     5761 ttcccatctt gggacaagac tttttattat acttgtagtt atatcagctc taagcaccat
     5821 tacttttcca tttacaacct ttaccataga ttcttctgga attttattat ttatagttgt
     5881 aaattcatca tattcttcaa ataattgtgg ttctatttga aaatattctt catccaaaaa
     5941 aagtttatct atttcccttt ttaatttata tcttttttta aaataaataa cctcatcttc
     6001 aatcctcaaa aatttcacat tctcaccctc ttcattaaat ttactcttat atttgcaaat
     6061 ttaaaacaat aaaaaaacca tataggcttt atacctatat ggctataatt ttctcttaaa
     6121 attacttccc atcatagata caagaccaat tacactaaaa acagggcttg tgtctatgaa
     6181 ttaatttcat attacaatcc ctttaatcta tgatgatggt gatgatttaa ttttgtattt
     6241 aattttacta tatttaatct caccatgata atttcctccc aaataatttt tattaatatt
     6301 ctaaatttac agttttttg
//
LOCUS       DAEPXF010000202         6268 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain HB27
            SAMN13376389-rid12479423.denovo.210, whole genome shotgun sequence.
ACCESSION   DAEPXF010000202 DAEPXF010000000
VERSION     DAEPXF010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13376389
            Sequence Read Archive: SRR10518432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6268)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6268)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001069624.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001069624.1
            Genome Coverage       :: 315x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/15/2021 01:54:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,165
            CDSs (total)                      :: 4,076
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 89
            rRNAs                             :: 10, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 10 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 19 of 69
            Pseudo Genes (incomplete)         :: 44 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 10 of 69
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6268
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13376389-rid12479423.denovo.210"
                     /strain="HB27"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="China: Hebei"
                     /collection_date="2017"
     gene            complement(<1..838)
                     /locus_tag="KRL67_003371"
     CDS             complement(<1..838)
                     /locus_tag="KRL67_003371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HBH1524212.1"
                     /translation="MKDKNTYFNRKGQENFIDIFLTDDIKKGANFKRHWHEHLQIYYF
                     IDGIAYLECGLDNFKIVPGNIVIANSNELHYLESLSDNLKFYSIRIEPTFLFSNQVDL
                     LQTKYLAPLALNHIVFNNLIQNDSLILKCITTILDEYHNKEVGYELSIKANIYQLIVL
                     LLRSHIDKIYSEDEFIKRKYSLEKFEGIFDLIEEKYMDKITLKELASRVNLSAHHFCR
                     IFKQITGKTTIDFINGIRLDKAIYYLKETDLNMTEIAMRCGFDSINYFSRLFKRNYNI
                     SPT"
     gene            980..2407
                     /locus_tag="KRL67_003372"
     CDS             980..2407
                     /locus_tag="KRL67_003372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBH1524213.1"
                     /translation="MTNSFKKDNNRKKITVLFVVIAMTFMATLDSSIINVALPVLASK
                     LNVSLASIEWVIASYSIIICSTLLFFGRLGDIIGKSRVFQAGTILFTSASLLCGLSNS
                     LTLLIVCRFIQGIGASAYMANNHGIITELFPKESRGKALGILVTAVAIGNMVGPSVGG
                     FILSIFDWNVIFFINIPIGLIVIFLNTKFLPNSKKSSENMDKTGAILQFLGTTLFFSA
                     LISAQQTGLLNPYILSALLLSIIFIILFLILEKKHPQPLLDLEIFRNFKFSLNLICAL
                     TSFICIASSSILIPFYLQSTMKLPPIQAGLFMILSPLILAIFSPIFGNISDKIKSEKI
                     ILIGLLVMSFGFFLMSRLKESSALILFVIYISIISIGQAIFQPANNALIMSNCSRSKL
                     GVVGSINSLVRNLGQVIGITISTTLLYNFMSIKAGYRVNDYVINNDKIFVFGMRNVYI
                     IVTLVCLIGAILISFYLFKYNKNEQ"
     gene            complement(2604..5198)
                     /gene="clpB"
                     /locus_tag="KRL67_003373"
     CDS             complement(2604..5198)
                     /gene="clpB"
                     /locus_tag="KRL67_003373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HBH1524214.1"
                     /translation="MDVEKMTLRVQKSLNEAYNEAVKNHNQQVDVIHLFSALINQEDG
                     LIPNIIEKMNISIDSLRNTVNFEIDKLPKVYGEGADSQGVSATRKINEVLIKAESISK
                     EFKDSYISVEHVMLAMMETESKSAVGKILKQYNINKNDFLNILSQVRGSQRVETQDPE
                     GTYDALARYGTNLVDLAKKNKLDPVIGRDEEIRRIIRILSRRTKNNPVLIGEPGVGKT
                     AIVEGLAERIVRGDVPEGLKDKIVFSLDMGALIAGAKYRGEFEERLKAVLKEVQSSDG
                     KIILFIDEIHTIVGAGKTEGSMDAGNLIKPMLARGELNCIGATTFDEYRKYIEKDKAL
                     ERRFQPVIAEEPTVEDTISILRGLKERFEIHHGVRIHDNAIVAAAKLSHRYIQDRFLP
                     DKAIDLIDEAGAMIRSEIDSLPTELDVVRRKLFTLETEREALLKENDDKSKSRLDDIQ
                     KEIAELKSKNDEMTAKYEKEKSQILDIKNLKAQLDEAKGKAEKYEREYDFNKAAEVKY
                     GEIPKLEEQIKQYEENMNDGSENSLLKEEVTEEEISSIVSKWTGIPVTKLVEGEREKL
                     LKLEDELHKRVIGQDEAVTAVSNAVIRARAGLKDERKPIGSFIFLGPTGVGKTELAKT
                     LARNLFDSEDNIIRIDMSEYMEKHAVSRLVGPPPGYVGYEEGGQLTEAVRRAPYSVIL
                     FDEIEKAHEDVFNMFLQILDDGRLTDNKGKTVDFKNTLIIMTSNIGSNYLLEAGGNIT
                     ETTNNLVMNEMKHRFKPEFLNRVDDIIMFKPLDQENIKKIIDIFMKDLKNRLKEKDIT
                     IEVTNSAKDVMVREGYDPVYGARPLKRYIGNTLETVIAKKLIAGDVYNGCTIVIDGKD
                     ENIEVLVK"
     gene            complement(5235..5831)
                     /locus_tag="KRL67_003374"
     CDS             complement(5235..5831)
                     /locus_tag="KRL67_003374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH1524215.1"
                     /translation="MSLKLDRNVLQWFDYVFENEKTSLRHYNFNCTLKEISSTSLNKV
                     AFILEKNNSRYWKLYFEIPAEVTLKLKQNIHPLFREYIYEQISLYNNNQIYNFVNSNI
                     LKVFNNIAIYQYNILENIYTIDFKKSFIDKCQYLLIGEKRLIDEDLYLIAKSKEVFDF
                     FNSDGTFNLTLSFDIQKNENLLDSLLELRKSIIINERI"
     gene            complement(5848..6252)
                     /locus_tag="KRL67_003375"
     CDS             complement(5848..6252)
                     /locus_tag="KRL67_003375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication/maintenance protein RepL"
                     /protein_id="HBH1524216.1"
                     /translation="MDKTLKKDLNQEYTESKSLVMNKSKYMNVCFKNYIKVVEGMYTK
                     RDLIPLKLINSMDKSNKIRKTLYELSCEFEYPKTSLSSLFTELKKMDFLQRVKNGEYM
                     INPHIAYKGSRADKESLLAEYNQIKRPNKVSK"
BASE COUNT         1992 a         1033 c          679 g         2564 t
ORIGIN      
        1 tagttggaga tatattataa ttccttttaa ataagcgact aaaataattt atgctatcaa
       61 agccgcatct catagcaatt tctgtcatat ttaaatctgt ttcctttaaa taatatatag
      121 ctttatcaag tctgattcca tttataaaat cgattgtagt cttacctgtt atttgcttaa
      181 atattctaca aaaatgatgt gcactcaagt tcacacgact agcaagttct tttaatgtaa
      241 ttttatccat atatttttct tctatcaaat caaagatacc ttcaaatttt tctaaagaat
      301 actttctttt tataaattca tcttcagaat atattttatc aatatgactt cttaataaga
      361 gtactattaa ttgatagata ttagctttta tagataactc atatccaact tctttattgt
      421 gatattcatc aagtatagtt gtaatacatt ttaaaatcag agaatcattt tgaattagat
      481 tattaaatac aatatgattt aaagccaatg gtgctaagta cttagtttgt aacaaatcaa
      541 cttgattact aaaaaggaat gtgggttcaa tacgaatact atagaatttt aaattatctg
      601 ataagctttc taaataatgt aattcattac tatttgctat tacaatgttt ccaggcacta
      661 tcttaaaatt atccaatcca cattcaagat atgctatacc atcaataaaa taatatattt
      721 gtaaatgctc atgccaatgc cttttaaagt ttgctccttt tttaatgtca tccgtcaaaa
      781 aaatatctat aaagttttct tgacctttcc tgttaaaata tgtattttta tccttcatta
      841 ttcccctctc atcaaaatag tgctaattat aagcaatata gttattgtat tcattacttc
      901 atatcgctat tatataaaat gtaactatca ttgtaaacat taacataatg ccagtcatta
      961 taataaggag gtaataaaaa tgacaaactc ttttaaaaaa gataataata gaaaaaaaat
     1021 cacagtttta tttgtagtca ttgccatgac ttttatggca acattagatt ctagtattat
     1081 aaatgttgct ctcccagttc ttgcaagcaa actaaatgta tctctagcat ctattgaatg
     1141 ggtaatagca agttactcaa ttattatttg ttcaacacta ttattttttg gaagacttgg
     1201 agatattatt ggtaaatcaa gagtattcca agcaggcact attctcttta catcagcttc
     1261 attactatgt ggattaagta attctttaac tttacttata gtttgccgct ttattcaagg
     1321 aataggcgct tctgcctaca tggcaaacaa tcatgggatt atcacagaac tatttccaaa
     1381 agaaagcaga ggaaaagccc tagggattct agttactgca gttgctattg ggaatatggt
     1441 tgggccatct gtgggaggat ttattttgtc aatatttgat tggaatgtta tattctttat
     1501 aaatattcca atcggtctta tcgtaatctt tttaaataca aaatttcttc ctaatagtaa
     1561 aaaatcttca gaaaatatgg ataaaactgg tgctatactt caatttttag gaacaacatt
     1621 attttttagt gctctaatta gtgctcaaca aacaggatta ctaaatccat atatattaag
     1681 tgcactatta ttatcaatta tttttattat attatttttg atattagaaa aaaaacatcc
     1741 tcaaccgctt cttgatcttg aaatttttag aaattttaaa ttttcattga atttaatttg
     1801 tgctcttaca tcattcattt gtattgcatc atcaagtatt ttaattccat tttatttaca
     1861 aagtactatg aaacttcccc ctattcaagc aggtttattt atgatactat cgcctttgat
     1921 tttagccatt ttttcaccta tatttgggaa tatatcagat aaaattaagt cagaaaaaat
     1981 tattttaatt ggtcttctcg ttatgagttt tggattcttt ctaatgtcaa gattgaaaga
     2041 aagttctgcc cttattttat ttgtaattta tatatcgata atatcaatag gacaagctat
     2101 atttcaacct gctaacaatg ctttgataat gtcaaactgt tctagaagta agctaggagt
     2161 tgtaggaagc ataaattccc ttgtaagaaa tttaggacaa gttataggta ttactatttc
     2221 tacaacactt ttatataatt ttatgagtat taaagctgga tatagggtca atgattatgt
     2281 gattaataat gataaaatat ttgtatttgg aatgagaaat gtttatatta ttgttacact
     2341 tgtttgctta ataggtgcaa tattaattag cttttatcta tttaaatata ataaaaatga
     2401 acaatagttt tttataatta tattcaaatt atgattatat tataagaaac ttactaaaat
     2461 actcccttag agttaagttt aatttgaaca tattaactta aaaacatttt attagtttga
     2521 taaaatattt cttttactat agtttataaa taaaaatcag ctgagcaaaa tatttggtat
     2581 attatgctca gctgattctc ttattattta actaaaactt caatattttc atcttttcca
     2641 tctataacta tagtacatcc attatacaca tctccagcaa taagtttctt tgcaattaca
     2701 gtttctaatg tatttcctat atatctctta agaggtcttg ctccatatac agggtcatat
     2761 ccttctctta ccataacatc ttttgctgaa tttgtaactt caatagtaat atctttttct
     2821 tttaatctat tttttaaatc tttcataaat atatctataa ttttcttgat attttcttgg
     2881 tctagaggct taaacattat aatatcatct actctattta aaaactcagg tttaaatcta
     2941 tgcttcattt cattcattac taaattgtta gtagtctctg ttatatttcc tcctgcttct
     3001 aatagataat tacttcctat attagaagtc attattataa gagtattttt aaagtctact
     3061 gtcttacctt tattatcagt aagtctacca tcatctaata tctgtaagaa catgttaaat
     3121 acatcctcat gtgctttttc tatctcatca aatagtatta cagaataagg agcacgtcta
     3181 actgcttctg taagttgacc accctcttca tatcccacgt accctggagg aggtccaact
     3241 agtcttgata cagcatgttt ttccatgtat tcagacatat ctattcttat tatattatct
     3301 tcactatcga ataaatttct agcaagagtt tttgcaagtt cagttttacc aacacctgta
     3361 ggtcctaaga aaataaatga accaattggt tttctttcat cttttaatcc tgctcttgca
     3421 cgtattactg catttgaaac tgctgtaact gcttcatctt gtccaataac tcttttatga
     3481 agttcatctt ctagtttaag taatttttct ctttctcctt caactagttt agttactggt
     3541 atacctgtcc actttgatac aatgctagat atttcttctt cagtaacttc ttcttttaaa
     3601 agagaatttt cagaaccatc attcatattt tcttcgtact gctttatttg ttcttctaac
     3661 ttaggtattt caccatattt aacctctgct gctttgttaa aatcatattc tctttcatac
     3721 ttttcagctt tacctttagc ttcgtctaat tgagccttta agtttttaat atctaatatt
     3781 tgactctttt ctttttcata ttttgctgtc atttcatcat ttttagattt taactctgct
     3841 atttcttttt gaatatcatc caatcttgat ttacttttat catcattttc tttaagtaat
     3901 gcttctcttt cagtttctaa tgtaaataac tttcttctaa caacatctaa ctctgttggt
     3961 aatgaatcta tttcactacg aatcatagct ccagcttcat caattaaatc aattgcttta
     4021 tctggtaaaa atctatcttg tatatatcta tgagaaagtt ttgccgctgc tacaatagca
     4081 ttatcatgta ttcttactcc atggtgtatc tcaaatcttt cttttaaacc tctaagtata
     4141 gatattgtat cttcaacagt aggttcctct gcaattacag gttgaaaacg tctttcaaga
     4201 gccttatctt tttctatata ctttctatat tcatcaaaag tagttgcacc aatacagttt
     4261 aattcacctc ttgcaagcat aggtttaata agattacctg catccattga accttctgtt
     4321 ttaccagctc caacgatagt atgaatctca tctataaata gtataatttt tccatcagaa
     4381 ctttgaactt cttttaagac tgcctttaat ctttcttcaa attcacctct atattttgca
     4441 ccagcaatta aagcacccat atcaagtgag aatacaattt tatcttttaa accttctggg
     4501 acatctcctc ttacaattct ctctgctaaa ccttctacaa ttgcagtttt acctacacca
     4561 ggctcaccaa tcaatacagg attattttta gttcttcttg ataaaattct tataattctt
     4621 cttatttctt catctcttcc aattacaggg tctaatttat ttttcttggc taaatctact
     4681 aagtttgttc catatctagc aagtgcatca tatgttcctt ctgggtcttg tgtttctacc
     4741 ctttgactac ctctcacttg tgataaaata tttaaaaagt cattcttatt tatattatat
     4801 tgttttaata tcttgccaac agctgatttt gattctgttt ccatcattgc aagcataaca
     4861 tgttctacac ttatatatga gtctttaaat tctttagaaa tactctctgc ctttataagc
     4921 acctcattta tttttctagt agctgatact ccttgtgagt cagcaccttc tccatatact
     4981 tttggaagtt tatcaatttc aaagtttaca gtatttctca aagaatcaat tgatatattc
     5041 attttttcga ttatatttgg tatcaatcca tcctcttgat taataagtgc tgagaaaaga
     5101 tgaattacat ctacttgttg gttatgattt tttactgctt cgttataagc ctcatttaaa
     5161 cttttttgaa ctcttaaagt cattttttct acgtccatat aaatccctcc attttaatat
     5221 tatatccaaa taaattaaat tctctcattt ataattattg attttctaag ctctaaaaga
     5281 ctatcaagta aattctcatt tttttgtata tcgaaactca atgttaaatt aaatgttcca
     5341 tctgaattaa aaaaatcgaa tacttctttt gattttgcta ttaaatacaa gtcttcatct
     5401 ataagtcttt tttcacctat taataaatac tgacatttat ctataaagct ctttttaaaa
     5461 tcaatagtgt atatattttc taaaatatta tattgataaa tagcaatatt attaaacact
     5521 tttaatatgt ttgaatttac aaaattgtaa atttgattat tgttatacag tgatatttgt
     5581 tcgtatatat attccctaaa taaaggatga atgttttgct taagttttaa agtaacttca
     5641 gctggtattt caaaataaag cttccagtac ctagagttat ttttttctaa aataaaagct
     5701 actttgttca aactggtaga actaatctcc tttagtgtac aattaaaatt atagtgtctc
     5761 aaactagttt tttcattctc aaatacataa tcaaaccact gtagaacatt tctatcaagt
     5821 tttaaactca tataaaaacc ttcttcctta tttactaacc ttattaggtc tttttatctg
     5881 attatattct gctaataaac tttctttatc agctctacta cctttatatg ctatatgtgg
     5941 atttatcata tactcaccat ttttcactct ttgtaaaaaa tccatttttt tcagctccgt
     6001 aaacaatgaa gataaagaag tttttggata ttcaaattca caagataatt catataatgt
     6061 ttttctaatc ttattacttt tatccataga gtttattaat tttaatggaa ttaaatccct
     6121 cttagtatac attccttcta ctactttaat ataattctta aaacaaacat tcatatactt
     6181 acttttattc ataactaaac ttttactctc tgtatattcc tgatttaaat ccttttttaa
     6241 tgtcttatcc aaattaaata tctcttct
//
LOCUS       DAEPXF010000203         6252 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain HB27
            SAMN13376389-rid12479423.denovo.211, whole genome shotgun sequence.
ACCESSION   DAEPXF010000203 DAEPXF010000000
VERSION     DAEPXF010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13376389
            Sequence Read Archive: SRR10518432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6252)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6252)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001069624.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001069624.1
            Genome Coverage       :: 315x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/15/2021 01:54:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,165
            CDSs (total)                      :: 4,076
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 89
            rRNAs                             :: 10, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 10 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 19 of 69
            Pseudo Genes (incomplete)         :: 44 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 10 of 69
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6252
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13376389-rid12479423.denovo.211"
                     /strain="HB27"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="China: Hebei"
                     /collection_date="2017"
     gene            79..795
                     /locus_tag="KRL67_003376"
     CDS             79..795
                     /locus_tag="KRL67_003376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PrsW family intramembrane metalloprotease"
                     /protein_id="HBH1524217.1"
                     /translation="MKLDLFLLAIVPILIGMFWIRSKDRYCREPLIHLIKFFLIGAFL
                     SVIIILLENLLMKFNVFEGYSELIYVSFVVAGLVEEGVKALILIPALIKEKHFTEKLD
                     GIIYSVFLALGFATIENMVYIFSESRNLALQVGINRAVISIPAHVMFAITMGYYISKY
                     KFEGNKNKRREYLFMAVLIPILLHGVFDFILMIEYRWAIILLIVYVIILWKINLDKLE
                     KYMNHSKKVFFGNLRKKKKK"
     gene            874..2184
                     /locus_tag="KRL67_003377"
     CDS             874..2184
                     /locus_tag="KRL67_003377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmB/NOP family class I SAM-dependent RNA
                     methyltransferase"
                     /protein_id="HBH1524218.1"
                     /translation="MTELPKKFLEDIEEILKDEYDDFIKSYEESKTTGLRINTLKIDK
                     KRLFDLELFDLNQIPWAEEGFYYDDKIDRPGRNPLHEAGAYYLQEPSAMSVIPKADIK
                     EGEKILDMCAAPGGKSTYILSKLNDTGLLVSNEINASRIKALGENLERFGARNCIITN
                     TDSNSLRKTFNGYFNKVIIDAPCSGQGMFRKDEVAISDWSYAKVVECQSIQKEIIRDG
                     YKMLKKDGILIYSTCTFSREENEHVINEFISEYPHAELIEMERLWPHKIKGEGHFVAK
                     IKKLEEEECNVKELKIKNLDKEIKDYREFEKKFLKVDFYSNKKNKFYLRGENLYLLPE
                     IYPDTKKLKVLRYGLHLGVLKKNRFEPSHALSHYLKVEDVKNVENFSVQSESILKYLK
                     GDVVNSNDSRGWVLVSVEGIPLGWGKESSGVIKNHYPKGLRKVF"
     gene            2284..2820
                     /gene="lepB"
                     /locus_tag="KRL67_003378"
     CDS             2284..2820
                     /gene="lepB"
                     /locus_tag="KRL67_003378"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HBH1524219.1"
                     /translation="MGEAVKKEVVEWIKVIVIALVLAFAITRFIVPTIVKGESMYPTL
                     VERDYLIVNRIAYKVGEPKYKDIIVFKTDLTEENGKKKDLVKRVIGVPGDHVKIQDSK
                     VYVNDKLLDETSYIHNNRTDGDIDIVVPEGKLFAMGDNREKSLDSRYDEVGLVDEHTI
                     LGKVLVRLYPFSKIGTID"
     gene            2986..3522
                     /gene="lepB"
                     /locus_tag="KRL67_003379"
     CDS             2986..3522
                     /gene="lepB"
                     /locus_tag="KRL67_003379"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HBH1524220.1"
                     /translation="MNETIKEEIVEWIKIIITALFFAFIITRFIKPTLVNGESMYPTL
                     KSHDYLVANRMTYKLSEPKCGDIMIFKTDLLQENGRKKELVKRVIGVPGDHLKIKDSK
                     VYINGKLLNEVSYIHDNYTEGDIDMVIPKGKVFAMGDNREVSLDSRYKEVGLVDEENI
                     KGKVILRVFPFTDIGIFE"
     gene            3532..4287
                     /locus_tag="KRL67_003380"
     CDS             3532..4287
                     /locus_tag="KRL67_003380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HBH1524221.1"
                     /translation="MAVGIASLLFNIEEALNICESIKQITHLEIGIDNISECSELCKY
                     KERISKLGLSIGIHLPMELNTCENIEYIRNSWINFIEKIESELKGFDIRYFNLHLGYV
                     MTNRVRKNRDKYLGNSVDFLDKLNTNSYVCIENTYSKGGDFSNIGNISYDFEYIFKRI
                     KNSKICFCYDTGHCLIDEDAYVKNLKDKIRLIHLSDNDGINDTHVGIGRGILSEEGIK
                     EVLTLDAEYLVLEINYEDIEDTISKLNNIVGEG"
     gene            4361..6028
                     /locus_tag="KRL67_003381"
     CDS             4361..6028
                     /locus_tag="KRL67_003381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside kinase"
                     /protein_id="HBH1524222.1"
                     /translation="MKKINLKIEGKDKEYSLEENSPGIRLGDIAKEFCDEHKGYITLA
                     VVDNKLKELNCRVKKDCEINFLDTTNEDGERVYFRVMSFIFVMACREIFWDSRVTIEH
                     SLSDGLYCEVHIDRKLKEADVENIKNKMKEIVNNDYVIEKIEVTRNEAISIFENNEMY
                     EKAELLKYKEYDSAKVYKCRNYIDHFYGYMLPSTGYIKSFDIKLYNGGVIILGPSEED
                     KTLPMKFIPQPKLSSVYYEAEEWSRLMGIDKVVSLNKIIENNEYGDIIRTFEALHEKK
                     LSQIADMIKDGNKRVILIAAPSSSGKTSFAHRLSIHLRVNNLNPISISLDDYFIDRKH
                     TPLDEYGNYDFESIYAIDLEKFNLDLKKLLAGEEIDDIRFNFKEGKREYTGKKIKIDS
                     NQPIILEGIHGLNPILTSSIPDEDKFKIYISALTQINLDDHNRIPTADLRMIRRIVRD
                     YNFRGYSADNTILQWASVRRGEKKNIFPYQEEADAIFNSACVYELAVLKRFAKPLLEE
                     IKEDNPAHIEATRLLKFLQYFVELDDTSDIPGISILREFIGGSKIVD"
BASE COUNT         2418 a          589 c         1214 g         2031 t
ORIGIN      
        1 ttaaaacacg aataagttaa aaataagtag atgatataaa tcataaataa atcaaaaatt
       61 ttgaaaggaa aataatctat gaagctagat ttatttttat tagcaatagt tcctatattg
      121 atagggatgt tttggataag gtctaaagat agatattgta gagaacctct aatccatcta
      181 attaaatttt ttttaatagg tgcttttcta agtgttataa ttatactttt agaaaattta
      241 ctgatgaagt ttaatgtatt tgaagggtat tcagaactaa tatatgtatc atttgtagtt
      301 gcaggacttg tagaagaagg tgtaaaagct ctgatactga ttcctgccct aattaaagaa
      361 aaacacttta ctgaaaagtt agatggtatt atttactctg tatttttagc tttaggattt
      421 gcaacaattg aaaatatggt ttatattttt tctgaaagta gaaatctagc actgcaagtt
      481 ggtattaata gggcagttat ctctatacca gctcatgtaa tgtttgcaat tactatgggc
      541 tactatattt ctaagtataa atttgaggga aataaaaata aaagacgtga gtatttgttt
      601 atggcagtgt taataccaat tttattacat ggagtttttg atttcatatt gatgatagaa
      661 tatagatggg ctattatatt acttatagtt tatgtaataa ttttgtggaa gataaatctt
      721 gataaacttg agaaatatat gaatcactct aaaaaagtgt ttttcggtaa tcttaggaaa
      781 aagaagaaga aataaaattt aaggtaaaga cataaataat taaagtataa taaatgacac
      841 acaagtaaag cagattgaag ggggattata gatttgacag aattaccaaa gaaattttta
      901 gaagatattg aagaaatact aaaagatgaa tatgatgatt ttataaaaag ttatgaggaa
      961 agtaagacta ctgggcttag aataaatact ttaaaaattg acaagaaaag attatttgat
     1021 ttggagttgt ttgatttgaa tcaaatacca tgggcagaag aaggttttta ttatgatgat
     1081 aaaattgaca gacctggtag aaatccactg catgaggctg gagcatacta cttgcaagag
     1141 ccttctgcaa tgagtgtaat accaaaggca gatattaaag agggtgaaaa aattctcgat
     1201 atgtgtgctg ctcctggtgg aaaatctaca tatatactat ctaagctaaa cgatacaggt
     1261 ttattggttt ctaatgaaat taatgctagt agaattaagg ctctagggga aaatttagag
     1321 cgatttggag cgagaaattg tattataaca aacaccgatt ctaatagctt aagaaagacg
     1381 tttaatgggt attttaataa ggttattata gatgcacctt gttctgggca aggtatgttt
     1441 aggaaggatg aggttgccat atccgattgg agttatgcaa aggtagtaga atgtcagtcc
     1501 atccaaaaag agattataag agatggatat aaaatgttaa agaaggatgg tattttaatt
     1561 tactcaactt gtactttttc aagagaagaa aatgagcatg ttataaatga gtttatcagt
     1621 gaatatccac atgcagaact tattgaaatg gaaagattat ggcctcataa gataaaggga
     1681 gaaggtcatt ttgttgccaa aataaaaaaa ttagaagaag aagaatgcaa tgtcaaagaa
     1741 ttaaaaataa aaaatttaga taaagaaata aaggattata gggagtttga aaagaaattt
     1801 ttaaaggttg atttttatag taataaaaaa aataaatttt atttaagagg agaaaatttg
     1861 tacctacttc ctgaaatata tcctgatacg aaaaaactaa aagtattgag atacggtttg
     1921 catttagggg ttttaaagaa gaatagattt gagccttctc atgcactatc tcattactta
     1981 aaagttgaag atgtaaaaaa tgttgaaaac ttcagtgtac aaagtgaatc aattcttaaa
     2041 tatttaaaag gggatgttgt aaattctaat gatagtagag gttgggtttt ggtttcggtt
     2101 gaaggaatac ctttaggttg gggaaaagaa tcttctggtg taattaagaa tcattatcct
     2161 aaaggtctta gaaaagtatt ttaaactatt agaaaaaaga agtataatat ttatattgta
     2221 ggtttatggt agattatttt gctgtagatt gacttaatat ttaacacaga ggagacgatt
     2281 ttagtgggtg aagcagttaa aaaagaagtt gtagaatgga taaaagtgat tgtcatagct
     2341 cttgttttgg catttgcaat aactcgtttt atagtgccaa caatagtcaa aggagaatca
     2401 atgtatccta cattagttga acgtgattat ttgatagtta atagaattgc gtacaaggta
     2461 ggagagccaa aatacaaaga tataatagta tttaaaaccg acttaacaga ggaaaatgga
     2521 aagaaaaaag atttagtaaa aagagttatc ggggttcctg gtgaccatgt aaaaatacaa
     2581 gactccaagg tatatgtaaa tgataaattg ttagatgaga cttcctatat acataataat
     2641 cgtactgatg gagatattga tatcgtagtt ccagaaggaa aattatttgc aatgggagat
     2701 aatagagaaa aaagtttaga tagtagatac gatgaggttg gattggtcga cgagcatacc
     2761 attttaggaa aggttctagt cagattgtat ccattttcta agataggaac tattgactaa
     2821 acatataaaa tttggagcaa tcgctgatta gctgaaatca ccagaccata cacaatagtt
     2881 gtctatataa aaaagacaac accttattta gtagacttat ctaaaagata gtgtttaaga
     2941 gacattatca ataaagataa tataactata aaggagaatt tgtgaatgaa tgaaactatt
     3001 aaagaagaga ttgtagagtg gataaaaata attattactg cacttttttt tgcatttatt
     3061 ataactcgtt ttataaaacc aacactagta aatggagaat caatgtaccc aacacttaaa
     3121 tcacatgatt atttggtagc aaacaggatg acatataagt tatcagaacc aaaatgtgga
     3181 gatataatga tatttaagac tgatttatta caagagaatg gaaggaaaaa agagcttgta
     3241 aaaagggtta taggtgttcc tggtgaccat ctaaaaatta aggattctaa ggtttatata
     3301 aatggtaagt tattaaatga agtttcatat atacatgata attatactga aggcgatatt
     3361 gatatggtga ttcctaaggg aaaagtattt gcgatgggag ataatagaga agttagttta
     3421 gacagtagat ataaagaagt gggactagta gatgaagaaa atattaaagg aaaagttatt
     3481 ttaagagtat ttccttttac agatataggt atttttgagt aggtgatttg tatggcagtt
     3541 ggtatagcat ccttattgtt taatattgag gaggctttaa acatttgtga gagtataaag
     3601 caaataactc atttagagat tggaattgac aatatatctg aatgtagtga gttatgtaaa
     3661 tataaagaga gaatttctaa gttaggatta tctataggga tacacttgcc tatggaatta
     3721 aatacttgcg aaaacataga gtatatacga aattcatgga taaattttat tgagaagata
     3781 gaatctgaac taaaaggatt tgatataagg tattttaacc ttcatttggg atatgttatg
     3841 acaaatagag ttagaaagaa tagggacaaa tatttaggaa atagtgtgga ttttttagac
     3901 aaattaaata caaattcata tgtatgtata gaaaatactt attcaaaagg tggggatttt
     3961 tcaaatattg gaaatatatc ttatgatttt gaatatatat ttaagaggat taaaaattct
     4021 aagatttgtt tttgttacga tactggacac tgtttaattg atgaggatgc ttatgtaaag
     4081 aacttaaagg ataaaataag actgatacac ttaagtgata atgatggtat caatgataca
     4141 catgttggta taggaagagg aattttatct gaagagggga ttaaagaggt cttaacatta
     4201 gatgcagaat atttagtatt agaaataaat tacgaagaca ttgaagatac tataagtaaa
     4261 ttgaataata ttgtaggaga gggttaaata cctctcttta tcttgttgat atgcctcatt
     4321 aattaaaatt tatattcatc aaaaggaagg agatgttgaa gtgaaaaaaa taaacttaaa
     4381 aatagaagga aaagataagg aatattcatt agaagaaaat tcccctggaa tcagattggg
     4441 agatatagca aaagaatttt gtgatgagca taagggttat ataacacttg cagttgtaga
     4501 caataaatta aaagagctaa attgtagagt taagaaggat tgtgagataa attttttaga
     4561 tactactaat gaagatggag agagagttta ctttagagtc atgtcgttta tatttgtaat
     4621 ggcttgtaga gaaatctttt gggatagcag agttacaata gaacattcac tatcagatgg
     4681 gctatattgt gaagttcata tagatagaaa gcttaaagaa gcagatgttg aaaatataaa
     4741 aaacaagatg aaagagatag taaacaacga ttatgttata gaaaaaattg aagttacaag
     4801 aaatgaagct atttctatct ttgaaaataa tgaaatgtat gaaaaggcag agcttttgaa
     4861 atacaaagag tatgatagtg ctaaagtata taagtgtaga aactatatag accattttta
     4921 tggttatatg ttgccatcaa ctggttatat aaaatcattt gatattaagc tttataatgg
     4981 aggcgttata attttagggc ctagtgaaga agataagact cttcctatga agtttatacc
     5041 tcaacctaag ctatcaagtg tatattatga agcggaagaa tggtcaagac ttatgggaat
     5101 tgacaaggta gtatctctta ataaaataat agaaaacaat gaatatggag atataataag
     5161 gacatttgaa gcattgcatg aaaagaagtt gtcccaaata gcggatatga taaaggatgg
     5221 caataagaga gtaattttaa tagcagctcc atcttcatct ggaaaaacaa gttttgctca
     5281 cagattatca atacatctta gagtaaataa tttaaatcct atttcaattt ctttagatga
     5341 ttattttata gatagaaaac atactccttt agatgaatat ggaaattatg attttgaatc
     5401 aatttatgca atagatttag agaagtttaa cttagactta aagaaacttt tagctggtga
     5461 agaaattgat gatataagat tcaattttaa agaaggtaag agagaataca ctggtaaaaa
     5521 gataaagata gattcaaatc agccaattat attagaaggt atacatgggc taaatcctat
     5581 tttaacatca tctataccag atgaagataa atttaagata tacataagtg cccttactca
     5641 aataaatttg gatgaccata atcgaatccc tactgctgat ttgagaatga taagaagaat
     5701 tgttagagac tataatttta gaggatatag tgctgataat actattttac aatgggcatc
     5761 agttagaaga ggagaaaaga agaatatatt cccgtaccaa gaggaggcag atgctatatt
     5821 taactctgct tgtgtatacg aattggcagt tttaaaacga tttgcgaaac ctttgttgga
     5881 agagataaag gaagataatc ctgcacatat tgaagctact agattactaa agtttttgca
     5941 gtattttgtt gaattggacg atacttctga cataccaggt atttcaattc ttagggaatt
     6001 tattggagga agtaaaatag ttgattaata tgtgtattat agattgtttt atgaagatta
     6061 aatggtatag caagtagtgt gattggttta tgtgaagtat attaagtgtc aagtggagaa
     6121 ttaaattgat acagatttta aagtttgagt tgatatataa aaaggcttgt tttatacttt
     6181 tgtatgaagc aagcttttta ttttaaattt tacttttttg tttgatattt tgttcgttta
     6241 tgtgataatt tt
//
LOCUS       DAEPXF010000204         6216 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain HB27
            SAMN13376389-rid12479423.denovo.212, whole genome shotgun sequence.
ACCESSION   DAEPXF010000204 DAEPXF010000000
VERSION     DAEPXF010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13376389
            Sequence Read Archive: SRR10518432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6216)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6216)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001069624.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001069624.1
            Genome Coverage       :: 315x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/15/2021 01:54:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,165
            CDSs (total)                      :: 4,076
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 89
            rRNAs                             :: 10, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 10 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 19 of 69
            Pseudo Genes (incomplete)         :: 44 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 10 of 69
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6216
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13376389-rid12479423.denovo.212"
                     /strain="HB27"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="China: Hebei"
                     /collection_date="2017"
     gene            125..775
                     /locus_tag="KRL67_003382"
     CDS             125..775
                     /locus_tag="KRL67_003382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HBH1524223.1"
                     /translation="MKAEKTSIYPIIKNNLNKISIVEYKKGQRFVASDKDLQEVFFII
                     EGVALVECTTRGGNKFLVDIVPENEFIGKISYIYEHNLKCDIFAKTNIKLFRFEKNVF
                     EEFYLKPDFIALFHRKCTRRIYELYKTRMVRELFSCTEVIAYCISNKQENNVCNIKAI
                     RNFSEIYSISRKSRYHSLLKLTEKGIVKKMGNTYEILNYDELHRLAFEVKEFLEDE"
     gene            complement(915..2291)
                     /locus_tag="KRL67_003383"
     CDS             complement(915..2291)
                     /locus_tag="KRL67_003383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HBH1524224.1"
                     /translation="MQELFSLKDENKRFYKILLSLCIPIIIQNLISTSVNVIDTIMIS
                     SLGETSVASVGVANQFFFLFNMSLSGITGGAGVFISQFYGKKDVSNIRKVTGLTCVLA
                     IVLSFVFVIPALLTPKPIIHIFSYDSEVVKLCIDYFSIAVFSYPLIAVSTVFSTGSRG
                     VRNPKLGMICSAFALVTNVILNYGFIFGNFGLPALGVKGAALATVIARICELILMITY
                     VYLYKKDYILKFGLKNLKAIDKIFIKSFSSKSFPIFVNDSVWAIGTVLYSVAYARAGT
                     SAIAASQIATSTGNFFIMTAVCIASGASIMLGNELGADHIKRAIEYAKKFSILVFSAG
                     LILGIILILNIPLLLKMFSVSDSLASDITKIFFIMGILMALKSFNTLVIIGILRSGGD
                     TKYALFLELGCMWLASIPLTFIAAFKGAPIFVLVLLTYSEEVVKFIFGVPRALSKKWA
                     INIVKEID"
     gene            complement(2307..4220)
                     /locus_tag="KRL67_003384"
     CDS             complement(2307..4220)
                     /locus_tag="KRL67_003384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HBH1524225.1"
                     /translation="MIVLSCNNLNKSFGIDSILENISFTVNEGDKIGIIGVNGTGKTT
                     LFKIISGIYGYDSGDIYTSKDCEIGYLEQNTNFYSDNTILEEVLEVFKNLIEMESYLR
                     ELEVKISEESTKTNSPIIEKIMDEYSHKLELFSDLNGYGYKSEAKGVLKGLGFSDNDM
                     DKPISILSGGEKTRVLLGKLLLKKPTLLLLDEPTNHLDSEAIEWLEVFLKQYKGTVML
                     ISHDRYFLDQSVNRIFEVHNKKLKVYNGNYSKFVELSKIEKELELKKFEDQQKEIKKQ
                     EESIERLKAYGREKHLKRARSKEKALDKVDVLDKPEAYRKKARIQFTPSVQSGNDVLQ
                     IRDVSMGYGERILFKDLDLDIYRGEKVALIGANGVGKSTLFKIITNELQPLSGNIKFG
                     TNVHVSYFHQEQKTLNLDNTIIDEIWENNTHLTQTTLRNMLGAFLFVDEEVFKKISTL
                     SGGERARVAILKLILSNANLLLLDEPTNHLDIDSKEVLEEALTNYDGTIFTISHDRYF
                     LNTVVDKILVLDENGITEYLGNYDYYIDKKRQIQEMSIIEEKEEKTRTQIKDEKRKER
                     EQREIEKKNRIKRQNIEKEIEKLEIEIEKLDILLCQEEVYSNPDKAKEVSQEKINLEN
                     NLSSLYDEWEEFM"
     gene            4356..4988
                     /locus_tag="KRL67_003385"
     CDS             4356..4988
                     /locus_tag="KRL67_003385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HBH1524226.1"
                     /translation="MLGNKNISMAVIRRLPKYHRYLGDLLDRDIQRISSKELSDIIGF
                     TASQIRQDLNNFGGFGQQGYGYNVEALHTEIGKILGLDRPYNAVLVGAGNLGQAIANY
                     AGFRKAGFEIKALFDANPRMIGLKIREFEVLDSDTLEDFIKNNNIDIAVLCIPKNGAQ
                     EVINRVVKAGIKGVWNFAPLDLEVPKGVIVENVNLTESLFTLSYLMKEGK"
     gene            5127..5666
                     /locus_tag="KRL67_003386"
     CDS             5127..5666
                     /locus_tag="KRL67_003386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA C4-type zinc finger protein"
                     /protein_id="HBH1524227.1"
                     /translation="MIFVEELWKKAIEFHGHECPGLAIGFKAALAARKYLGCKQSGDE
                     EIVCISENDACGIDGIQVVLSCTMGKGNLLLRMTGKSVYHFFNRENGKSIRIYVKSKP
                     FEMDREGYKRYLLESDFEEIFEITDTKLRLPEKARIFKSLKCEVCGEFASEQYIRIEE
                     GKKVCLDCFDKYERFYEEY"
     gene            complement(5843..6013)
                     /locus_tag="KRL67_003387"
     CDS             complement(5843..6013)
                     /locus_tag="KRL67_003387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004619190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HBH1524228.1"
                     /translation="MAYKITDACISCGACEAECPVSCISAGDDAYVIDAGSCIDCGSC
                     AGACPVDAPQPE"
BASE COUNT         2294 a          854 c          878 g         2190 t
ORIGIN      
        1 gcgtgagtag aatggatttg tcaagtttaa tttagttgta attctatctt ttattaaaaa
       61 taacataaat taaaacatat aaataaaata ttttatacaa tatattgtaa aggaggtaac
      121 tttgatgaaa gcagaaaaaa caagtatata tccaattatt aaaaataatt taaacaaaat
      181 atcaattgtt gaatataaaa agggtcaaag atttgtagca tcagataaag acttacaaga
      241 agtgttcttt ataatagagg gagttgcatt agtggagtgt actactagag gaggaaataa
      301 atttcttgtt gacattgtac ctgaaaatga atttataggc aaaatcagtt atatatatga
      361 acataatttg aaatgtgata tatttgcaaa gaccaatata aaattatttc gatttgaaaa
      421 aaatgtattt gaagagtttt atctcaaacc agattttatt gcactatttc acagaaaatg
      481 tacaagaaga atatatgaat tatataaaac tagaatggta agagaactat tttcttgcac
      541 agaagttatt gcctattgta tatcaaataa acaagaaaat aatgtttgca atataaaagc
      601 tattcgtaat ttcagtgaaa tctattcaat tagtagaaaa agtagatacc attctctgtt
      661 aaagttaact gaaaaaggga tagtaaaaaa aatgggtaat acatatgaaa ttttaaacta
      721 tgatgaatta catagattgg catttgaagt aaaagagttt ctggaagatg agtaaagatt
      781 gttacttaat caaaataagg tataataatt tctgtaaaaa atagaaatag tctcactgaa
      841 aatcaaaaat actttagtta ttttcaatga gactgtttag aaatattgta taattacaat
      901 gtatagtata attattagtc aatttccttt actatattaa tagcccattt tttagataat
      961 gctctaggaa cgccaaatat aaacttaacc acttcttcac tataagtgag taaaacgagt
     1021 acaaatatag gtgcaccttt aaatgcagct ataaaagtta gaggaataga agctaaccac
     1081 atacatccta attctaaaaa tagagcatat ttagtatctc ctccacttct taaaatacca
     1141 attataacca atgtattaaa agatttaaga gccattagta ttcccattat aaagaatatc
     1201 ttagttatat cagaagctaa gctatcagaa acactaaaca tttttaataa taatggtata
     1261 tttaaaatta aaataattcc aagtattaaa ccagctgaaa atacaagtat agaaaacttt
     1321 ttagcatatt caatggctct tttaatatgg tcagctccaa gttcatttcc aagcataata
     1381 gatgcaccag atgcaataca tacagctgtc attatgaaga aattacctgt actggttgct
     1441 atctggctag cagctatagc agaagtacca gctcttgcat atgcaacaga atacaggaca
     1501 gttccaattg cccatacaga gtcatttaca aatataggaa agctttttga tgaaaaagat
     1561 tttataaaaa ttttatcaat agcttttaga ttttttagac caaactttaa tatatagtct
     1621 tttttataaa ggtaaacata agtaatcatt aaaattaatt cacatattct agcaataaca
     1681 gtagctagag cagcaccttt tacacccaat gcaggaagac cgaagttacc aaatataaat
     1741 ccataattta aaataacatt tgttacaagt gcaaatgcac tacaaatcat acctaactta
     1801 ggatttctaa cacctcttga accagtacta aatacagtac ttacagcaat taatggataa
     1861 ctaaatactg cgatactaaa gtaatctata caaagtttta ctacctcaga atcatacgaa
     1921 aatatgtgta taattggctt tggtgttagt aaagcaggaa taacaaatac aaaacttaaa
     1981 acaattgcca atacacaagt aagacctgtg acttttctta tattactcac atccttcttt
     2041 ccataaaact gtgaaataaa aactccagca cctcctgtga taccagataa ggacatatta
     2101 aaaagaaaga aaaattggtt tgcaactcca actgaagcta cagatgtttc ccccaaacta
     2161 cttatcataa ttgtatctat tacatttaca gaagtagaaa ttaagttctg tatgataata
     2221 ggtatacata aagatagaag tattttataa aatcttttat tttcgtcttt taaagaaaat
     2281 agttcttgca aataatcctc tcctttttac ataaattctt cccactcatc atatagagag
     2341 gaaaggttat tttctaaatt aattttttct tggctaactt ctttagcttt atctggatta
     2401 gagtatactt cttcttgaca taaaagtata tctaattttt caatttcaat ctctaacttt
     2461 tcaatttctt tttctatatt ttgtcttttt attctatttt tcttttctat ttctctttgt
     2521 tctctctctt ttcttttttc atcttttatt tgagttctag ttttttcttc tttttcttct
     2581 ataatactca tctcttggat ttgccttttt ttatctatat aataatcata atttcccaga
     2641 tattcagtaa ttccattttc atctaaaact aaaattttat caacaacagt atttagaaag
     2701 tatctatcat gtgatattgt aaatatagtc ccatcataat tagttaatgc ctcttcaagg
     2761 acttccttag aatcaatatc taggtgatta gtaggctcat ctaataacaa taagtttgca
     2821 tttgatagta taagtttaag tatggcaact ctagctcttt ctccaccact caatgtagaa
     2881 atcttcttaa aaacttcttc atctacaaat aaaaatgccc ctaacatatt tctcaaagta
     2941 gtttgagtaa gatgagtatt attttcccaa atctcatcaa ttattgtgtt atctaaatta
     3001 agagtttttt gttcctgatg gaagtaggaa acatgtacat tagttccaaa tttaatattg
     3061 ccactaagag gttgtagttc attagttatt attttaaata atgtagattt accaacacca
     3121 tttgcaccta taagtgcaac tttttcccct ctatatatat ctaaatccaa gtctttaaaa
     3181 agtattcttt ctccataacc catagatacg tctctaattt gaagaacatc attaccactc
     3241 tggacagaag gcgtaaattg tatacgagct tttttcctat aagcttctgg tttatctaaa
     3301 acgtctacct tgtctaatgc tttttcttta cttcttgcac gctttaggtg tttttctcta
     3361 ccataagctt ttagtctttc aattgattct tcttgctttt ttatttcttt ttgttggtct
     3421 tcaaatttct taagctctag ttctttttct attttagata actcaacaaa tttagagtag
     3481 ttaccattat atactttaag ttttttgttg tgaacttcaa aaattctatt tacggattgg
     3541 tctaagaaat atctatcatg agatattaac ataacagtcc ctttatattg ttttaaaaat
     3601 acttctaacc attctattgc ttcagagtct aagtggttag taggttcatc aagtaacaat
     3661 agagttggtt tcttaagaag taattttcct aaaagaactc tagttttttc accaccagaa
     3721 agtatgctta ttggcttatc catatcattg tcactaaatc ctaatccttt tagaactcct
     3781 ttggcttcag atttatatcc atatccattt aaatctgaaa ataattctag tttatgtgaa
     3841 tattcatcca tgattttttc tataattgga gagtttgttt ttgtactttc ttcagatatt
     3901 ttaacctcca attctcttaa ataggattcc atttctatca aatttttaaa aacttctagt
     3961 acttcttcca aaatagtatt atccgaataa aaattggtat tttgttctaa atatccgatt
     4021 tcacaatctt tagaagtgta aatatctcca ctatcatatc cataaattcc tgaaattatt
     4081 ttaaataaag tagtttttcc agtaccatta acgccgatta taccaatttt atcgccctca
     4141 tttactgtaa aactaatatt ttctaatata gaatctatac caaaactttt atttaagtta
     4201 ttacatgaca aaacaatcat aaatttctac ctccttgaac tatctaaaat tataatgttt
     4261 tgatataata taatcaaggt tatatgtatt agttttgaaa aagaaatatt ttattgtgaa
     4321 tcaaacaaaa aagaggacac ttttaggagg tgaagatgtt gggaaataaa aatatatcaa
     4381 tggcagttat aagaaggctc ccaaaatatc atagatatct tggagactta ttagataggg
     4441 atatacaaag aatatcttct aaagaattga gtgatataat agggtttacc gcttctcaaa
     4501 taagacaaga tttaaacaac tttggtggat ttggacaaca aggatatggt tataatgtag
     4561 aagctcttca tactgagata ggtaaaattc ttgggttgga tcgaccatac aacgcagttc
     4621 ttgtaggagc aggtaactta ggacaagcta tagccaatta tgcaggattt agaaaagctg
     4681 gattcgagat aaaagcttta tttgatgcaa atcctagaat gataggttta aagataagag
     4741 agtttgaagt attagattca gatactttag aagactttat aaaaaacaat aatatagata
     4801 ttgctgtatt atgtatacct aaaaatggag cacaagaagt tattaataga gttgtaaaag
     4861 ctggaattaa aggtgtatgg aattttgcac ctttagattt agaagttccg aaaggtgtta
     4921 tagttgaaaa tgtaaactta acagaaagtt tatttacttt atcgtattta atgaaagaag
     4981 gaaagtagac aacttaaaca tattaataat gagtagttgt gaagagtatt gagaaagtca
     5041 gaagatttaa tattaaatcc tctggctttt tgaaatatta ggataaaaat ttaaataatg
     5101 tatggaaata aacaatatta aggagtgtga tttttgtgga ggaattatgg aaaaaggcaa
     5161 ttgaatttca tggtcatgaa tgtccaggct tagctatagg atttaaagcg gcacttgcag
     5221 caagaaaata tttaggatgt aagcagtcag gagatgagga aattgtatgt atttctgaaa
     5281 atgatgcttg tggaatagat ggtatacaag tagtgctaag ttgcacaatg ggtaagggaa
     5341 atttactact taggatgact gggaaatcag tatatcattt ctttaataga gaaaatggaa
     5401 aatctataag aatatatgta aagtcaaagc catttgagat ggatagagaa ggatacaaaa
     5461 gatacttatt agaaagtgat tttgaagaga tatttgagat tacagatact aagttaagac
     5521 ttcctgaaaa agcaagaata tttaaatcac ttaaatgtga agtgtgtggt gaatttgcat
     5581 ctgagcaata tataaggatt gaagaaggaa agaaagtatg tcttgattgc tttgataaat
     5641 atgaaagatt ttatgaggag tattaaaatt ataaattaga ttaaagttaa aagtattaga
     5701 tatgtgctaa cagtatttta agatgaatta ggtagcaact ataataaaat atataaataa
     5761 tattacttga atcatacata attaaaaaga ggctgattag tatttagaaa ataaaatcag
     5821 tctctttatg aataacgaat tattattctg gttgaggtgc atctacagga caagctcctg
     5881 cacaagaacc acagtctata caagaaccag catctataac atatgcgtcg tctccagctg
     5941 atatacagct aacagggcat tcagcctcac aagcaccaca gcttatacaa gcgtcagtaa
     6001 ttttatatgc cattttgaaa tcctcccttt tatatgtatt gtaattcaat aacattataa
     6061 caaatattat tgaatataac aattaaatta attctacaac aaaaacttta ttatgatatt
     6121 cttgttttaa agttttacaa taaagtttaa tctgcttata tttttattta ttttaaagaa
     6181 gtttatttta aaaataaaat taaatttaaa aagtta
//
LOCUS       DAEPXF010000205         6194 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain HB27
            SAMN13376389-rid12479423.denovo.213, whole genome shotgun sequence.
ACCESSION   DAEPXF010000205 DAEPXF010000000
VERSION     DAEPXF010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13376389
            Sequence Read Archive: SRR10518432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6194)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6194)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001069624.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001069624.1
            Genome Coverage       :: 315x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/15/2021 01:54:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,165
            CDSs (total)                      :: 4,076
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 89
            rRNAs                             :: 10, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 10 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 19 of 69
            Pseudo Genes (incomplete)         :: 44 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 10 of 69
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6194
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13376389-rid12479423.denovo.213"
                     /strain="HB27"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="China: Hebei"
                     /collection_date="2017"
     gene            183..1205
                     /locus_tag="KRL67_003388"
     CDS             183..1205
                     /locus_tag="KRL67_003388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HBH1524229.1"
                     /translation="MKNLLEIQKKLIPQAIELMERRYSILRQISLSEPIGRRTLSNVL
                     EISERIIRSETEFLKEQGLIDVAVSGMTITKEGSDLLDKLKDIMSDIMGLSKLQDRVR
                     EKLGIKRVILVPGSCDENGSLLKDVTRYGCEYFLGILKDGDIVSITGGSTMLEFSNVI
                     KTDKRYPNSTIVPARGSMGTDVEMQSNNIVASISKKLHSNCKLLHIPDELDESAMKTL
                     SQVPEIKTTLEYIKRTDILVFSIGRADVMAKRRKLPEDKVDEILSKNAVGEAFGYYFN
                     KDGEIVYKLNTVGIDLETVKQVKETIAIFAGTEKVEALLAISNMHKNMVLVTDEESAY
                     KILSLL"
     gene            1265..2272
                     /gene="gap"
                     /locus_tag="KRL67_003389"
     CDS             1265..2272
                     /gene="gap"
                     /locus_tag="KRL67_003389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HBH1524230.1"
                     /translation="MVKVAINGFGRIGRLALRKMMEQQDKFEVVAINDLTDAKMLAHL
                     FKYDTAQGRFNGEIEVKEGAFVVNGKEIKVTAERNPADLPWAELGVDIVLECTGFFTS
                     KDKAEAHIQAGAKKVVISAPATGDLKTIVFNTNSDILDGSETVISGASCTTNCLAPMA
                     KVLNDKYGIEKGLMTTIHAYTNDQNTLDGPHPKGDLRRARAAAGNIVPNTTGAAKAIG
                     LVIPSLKGKLDGAAQRVPVVTGSITELVCTLGKNVTVEEINAAMKEASNESFGYTEEM
                     LVSSDIIGISYGSLFDATQTKVMEVDGKQLVKVVSWYDNEMSYTSQLIRTLGYFAQLA
                     K"
     gene            2494..3696
                     /locus_tag="KRL67_003390"
     CDS             2494..3696
                     /locus_tag="KRL67_003390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HBH1524231.1"
                     /translation="MSMLNKKTIEDIDVCGKKVLVRCDFNVPLQDGVITDENRLNGAL
                     PTIQYLISKGAKVILCSHLGKPKGEAKPELSLAPVAKRLSEMLGKEVVFAADDNVVGE
                     NAKKATEKMENGDVVLLENTRYRKEETKNEENFSKELASLAEIFVNDAFGTAHRAHCS
                     TVGAGEFLQERVCGYLIQKELKFLGEAVANPVRPFTAILGGAKVSDKLAVINELLEKV
                     DNLIIGGGMAYTFLKAQGYEVGTSLLEIDKVEYAKEMMEKAKNKGVNLLLPVDVVMAD
                     HFAPDATPIVTEDANVKEDYMGLDMGPKTIANFVKTIKESKTVVWNGPMGVFEFENFA
                     NGTLSVARAMAELTDATTVIGGGDSAAAVNQLGFGDKMTHVSTGGGASLEFLEGKELP
                     GIAALDNK"
     gene            3722..4465
                     /locus_tag="KRL67_003391"
     CDS             3722..4465
                     /locus_tag="KRL67_003391"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HBH1524232.1"
                     /translation="MRKPIIAGNWKMHKTIKEALEFVNEVKYKVNSDKVEAVICAPFT
                     LLKDLKEATKGTNIKIGAQNMHFEEKGAFTGEVSPLMLKEIDMDYVVIGHSERRQYFN
                     ETDETVNKKVLKALEVGIDPILCVGETLEQREAGKTKDVCKIQVEKALENVLKDDLAK
                     VVVAYEPIWAIGTGKTATAEDANDVISYIREVIKGLYGELANEVRIQYGGSVKPSNVA
                     EIMGQSDIDGALVGGASLASNDYLDLVNF"
     gene            4474..6006
                     /locus_tag="KRL67_003392"
     CDS             4474..6006
                     /locus_tag="KRL67_003392"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003228330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HBH1524233.1"
                     /translation="MMKKPVALIIMDGFGYNKDVKGNAIAESKTPNLDRIKKEYPNTL
                     INASGLDVGLPDGQMGNSEVGHTNIGAGRIVYQDLTRITKSIKDGDFFTNKVLCEAMD
                     NAKENSLHVMGLLSDGGVHSHIDHLKAIIKMSKDKGVQKVYVHAFTDGRDTDPQSALE
                     YAKEVQASMDEIGVGEFATVSGRYYAMDRDKRWERVELAYNAMVRGIGEKANSIEEAI
                     QNSYDDGKNDEFIMPTVIMKDDKPVGSIKENDSIIFFNFRPDRARQITRALVCEEFDG
                     FKREDIKSFFVCLTEYDITIENVHIAFGPQSLANTLGEYLAKNGKTQLRAAETEKYAH
                     VTFFFNGGVEEPNKGEERLLIPSPKVATYDLKPEMSAYELTDKALEKLGEDKFDFIVL
                     NFANPDMVGHTGSIEAAVKAVETVDTCVGKLVDKIVELGGSAIITADHGNAEYMLDPE
                     TGKTVTAHSINPVPFIVVGQEYESAKLLDGGRLSDIAPTVLDMMKLEKPEEMTGHSLI
                     SK"
BASE COUNT         2462 a          723 c         1254 g         1755 t
ORIGIN      
        1 aagaaaaaat actgtgattt gaaaatggtg gagaaatatt ttataaaata aaaaaataaa
       61 aaagtttttg aaaaactatt gaaatctata cgcaaatcct ataaaataaa aatatcttgt
      121 gggacttaaa taaacactag ggacgaaaaa agtcccgaaa ggaaagcaat aaggagtatg
      181 ttatgaagaa cttattagaa attcaaaaga aattgattcc acaggcaatt gaacttatgg
      241 aaagaagata ctctatttta agacaaatat ctttaagtga accaataggt aggagaacat
      301 tatcaaatgt attagagatt agtgaaagaa taatcaggtc agaaactgag tttttaaaag
      361 aacaaggact tatagacgta gctgtatctg ggatgactat cactaaagaa ggttctgatt
      421 tgttagataa acttaaggac atcatgagtg acatcatggg attgtctaag ttacaagata
      481 gagtcagaga aaagcttgga ataaaaaggg taatattagt tcctggaagc tgtgatgaaa
      541 atggaagttt actaaaagat gtaactagat atggatgcga gtatttctta ggtattttaa
      601 aagatggaga tatcgtttca ataacaggag gaagcactat gttagagttt tcaaacgtta
      661 ttaaaactga taagagatac ccaaactcta caatagttcc agctagagga agtatgggaa
      721 ctgatgtaga gatgcaatct aataatatag ttgcttcaat aagtaagaaa ttacattcta
      781 attgcaaact tttacatata ccagatgagt tggatgaaag tgcaatgaaa actcttagcc
      841 aagtgccaga gattaagaca actttggagt atattaaaag aacagacatt ttagttttta
      901 gtataggtag agcagatgtc atggcaaaga gaagaaaact tccagaagac aaggttgatg
      961 agatattatc taaaaatgca gttggagaag catttggata ttatttcaac aaagatggag
     1021 aaatagttta caaactgaac actgttggaa ttgatttaga aacagttaaa caagtaaaag
     1081 aaactattgc aatctttgca ggtactgaaa aagtagaagc cttattagct atatcaaata
     1141 tgcataaaaa catggttctt gtaacagatg aagaaagtgc ttataagata ttgtcattat
     1201 tataaaagtc aagacctagc acagagctag aaaacataca tttactttat aggaggtagc
     1261 gaaaatggtt aaagtagcta taaatggatt tggaagaata ggaagattag cattaagaaa
     1321 aatgatggag caacaagata agttcgaagt tgttgcaata aacgacttaa cagacgctaa
     1381 aatgttagct catttattta aatatgatac agctcaagga agatttaacg gagaaataga
     1441 agttaaagaa ggtgcttttg tagtaaacgg aaaagaaata aaagtaacag cagaaagaaa
     1501 tcctgctgac ctaccatggg ctgaattagg agtagatata gtattagaat gtacaggatt
     1561 ctttacatct aaagataaag ctgaagctca tatacaagca ggggctaaga aagttgttat
     1621 atctgcacca gctactggag atttaaaaac aatagtattc aacacaaaca gtgatatatt
     1681 agatggttct gaaacagtta tatcaggagc ttcttgtact actaactgtt tagctccaat
     1741 ggctaaagta ttaaatgata aatacggtat agaaaaagga ttaatgacta ctatacatgc
     1801 atacactaat gaccaaaata ctttagatgg tcctcatcca aaaggtgatt taagaagagc
     1861 tagagctgct gctggaaaca tagttccaaa cactacagga gctgcaaaag caataggctt
     1921 agtaatacct tcattaaaag gaaaattaga tggggcagca caaagagtac cagttgtaac
     1981 tggttcaata actgaattag tttgtacatt aggaaaaaat gttacagttg aagaaataaa
     2041 tgcagctatg aaagaagctt caaacgaatc tttcggatat actgaagaaa tgttagtatc
     2101 ttctgacata atcggaataa gctacggttc attatttgac gctactcaaa caaaagttat
     2161 ggaagtagat ggaaaacaat tagttaaagt tgtttcttgg tatgataatg aaatgtctta
     2221 tacttctcaa ttaataagaa ctttaggata ctttgcacaa ttagcaaaat agtcagtttt
     2281 gagaaaatag aaaagtagat tgacaaagtc cgagtttgta gtttaatact acggactcag
     2341 gacttttttt aagaaaaaat atataaaata cacctaaata atgttacata ctatatataa
     2401 aaatatgata atatgatata tttaataggg taaaagtatg tactatatta tacttggcaa
     2461 taaaaaatta ctttattaaa aggagagata tttatgtcaa tgcttaacaa aaaaactata
     2521 gaagatattg atgtgtgcgg aaaaaaagta ttagtaagat gtgactttaa tgtgccatta
     2581 caagatggtg taattactga tgaaaataga ttgaacggag ctttaccaac tattcaatat
     2641 ttaatttcta aaggtgcaaa agttatatta tgctctcact taggaaagcc aaaaggagaa
     2701 gctaagccag aattatcttt agcaccagta gcaaagagat tatctgagat gttaggaaaa
     2761 gaagttgtat ttgcagcaga cgataatgtt gtaggagaaa atgcaaagaa agcaactgaa
     2821 aaaatggaaa acggagatgt agtattatta gaaaatacta gatatagaaa agaagaaact
     2881 aaaaacgaag agaatttctc taaagaatta gcatcacttg ctgaaatatt tgtaaatgat
     2941 gcgtttggaa ctgctcatag agcacattgc tcaacagttg gtgcaggtga gttcttacaa
     3001 gagagagttt gtggatactt aattcaaaaa gaattaaaat tcttaggaga agctgttgca
     3061 aaccctgtaa gaccatttac agctatactt ggaggagcta aagtttctga taaattagct
     3121 gttataaacg aattattaga aaaagttgat aatctaataa taggtggagg aatggcttat
     3181 acattcttaa aagctcaggg ttatgaagta ggaacttctt tacttgaaat agataaagtt
     3241 gaatatgcta aagaaatgat ggaaaaagct aagaataagg gtgttaactt attattacca
     3301 gtagacgttg ttatggcaga ccactttgca ccagatgcta ctccaatagt aacagaagat
     3361 gctaatgtaa aagaagatta catgggacta gatatgggac ctaagactat agcaaacttt
     3421 gtaaaaacta taaaagaatc aaaaactgta gtatggaatg gaccaatggg agtatttgaa
     3481 tttgaaaact ttgcaaatgg tacattgtca gtagctagag ctatggcaga gttaactgat
     3541 gcaactactg taattggtgg tggagatagt gcagcagcag ttaatcaatt aggatttggt
     3601 gataagatga ctcacgtatc aacaggtgga ggagcatcac tagaattttt agaaggtaaa
     3661 gaattaccag gtattgcagc attagataat aaataaacta atatatagag gagattgtaa
     3721 aatgagaaaa cctataattg caggaaactg gaaaatgcat aaaacaataa aagaagcttt
     3781 agaatttgta aatgaagtta aatataaagt aaattctgat aaagtagaag ctgttatatg
     3841 tgcaccattt actttattaa aggatttaaa agaagctact aagggtacaa atataaagat
     3901 aggtgctcaa aatatgcact ttgaagaaaa aggagcattt acaggagaag tttcacctct
     3961 tatgttaaaa gaaatagata tggactatgt tgtaatagga cattcagaaa gaagacaata
     4021 cttcaatgaa actgatgaaa ctgtaaacaa gaaagtttta aaagcattag aagtaggaat
     4081 agacccaata ttatgtgttg gagaaacttt agagcaaaga gaagctggaa aaactaaaga
     4141 cgtttgtaaa attcaagttg aaaaagcttt agaaaatgtt cttaaggatg atttagcaaa
     4201 agtagttgtt gcatatgagc caatctgggc tattggaact ggtaaaactg caactgctga
     4261 agatgctaat gacgttatat cttatataag agaagttata aaaggattat atggagaatt
     4321 agctaatgaa gttagaatac aatacggtgg aagtgttaaa ccttcaaatg tagccgaaat
     4381 aatgggtcaa agtgatatag atggagcttt agtaggtgga gctagtttag ctagtaatga
     4441 ttacctagac cttgttaatt tctaaggagt gaaatgatga aaaaaccagt tgctttaatt
     4501 attatggacg ggtttggata caataaggat gttaagggaa atgcaattgc tgagtccaaa
     4561 actcctaatt tagatagaat aaaaaaagag tatccaaaca cattgataaa tgctagtggt
     4621 cttgatgttg gtctacctga tggtcaaatg ggaaattcag aagttggaca cactaacata
     4681 ggtgcaggta gaatagtata tcaagattta actagaataa caaaatctat aaaagatggt
     4741 gactttttta ctaataaagt tttatgtgaa gcaatggata atgcaaaaga aaattctctt
     4801 catgtaatgg gacttttgtc tgatggagga gtacattcac atatagacca tcttaaggct
     4861 ataatcaaaa tgtcaaaaga taaaggcgta caaaaggtgt atgttcacgc atttactgat
     4921 ggtagagata ctgaccctca aagtgcatta gaatatgcca aagaagtaca agctagtatg
     4981 gatgaaattg gagtaggtga atttgctaca gtttctggta gatactacgc tatggataga
     5041 gataagagat gggaaagagt agagcttgca tacaatgcta tggttagagg tattggtgaa
     5101 aaagcaaatt ctattgaaga agctattcaa aactcttatg atgatggaaa aaatgatgag
     5161 tttataatgc caacagttat tatgaaagat gataaaccag ttggaagtat aaaggaaaat
     5221 gattcaatta tattcttcaa ctttagacca gatagagcta gacaaataac tagagcttta
     5281 gtttgtgaag agtttgatgg atttaaaaga gaagatatta aaagcttctt tgtatgctta
     5341 acagagtacg atataactat agagaatgtg catatagcat ttggacctca atctttagcc
     5401 aacactctag gtgagtatct agctaaaaat ggaaaaactc aacttagagc tgctgaaact
     5461 gaaaagtatg cacatgtaac tttcttcttc aatggtggag tagaagagcc aaataaaggt
     5521 gaagaaagac tattgatacc ttctccaaag gttgcaactt atgatttaaa accagaaatg
     5581 tcagcatatg aattgacaga taaagcttta gagaaattag gagaagataa gtttgacttt
     5641 atagtgttaa actttgcaaa tcctgatatg gtaggtcaca caggaagtat agaagcggct
     5701 gtaaaggctg ttgaaactgt agacacttgt gtaggaaaat tggtagataa aatagtagag
     5761 ttaggtggaa gtgcaataat aactgctgac cacggaaatg ctgaatatat gttagaccca
     5821 gaaacaggaa aaactgtaac agctcactct attaatccag taccatttat tgtagttggt
     5881 caagaatatg aaagtgcaaa attattggat ggaggaagat tatctgatat agcaccgact
     5941 gttttagata tgatgaagtt agaaaagcca gaagaaatga ctggtcattc acttatttca
     6001 aaatagtcga ttctgataag tagagaataa tatataaact acaatataaa tttaaaaata
     6061 acacagttta ggtttatttt gtaaggttta gactttatga cataagattt agattttgta
     6121 aggtctggat attgtaaggt gtagatgaaa gtatatttta taaaatttac aagatagaca
     6181 atttataaaa taaa
//
LOCUS       DAEPXF010000206         6128 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain HB27
            SAMN13376389-rid12479423.denovo.214, whole genome shotgun sequence.
ACCESSION   DAEPXF010000206 DAEPXF010000000
VERSION     DAEPXF010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13376389
            Sequence Read Archive: SRR10518432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6128)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6128)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001069624.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001069624.1
            Genome Coverage       :: 315x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/15/2021 01:54:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,165
            CDSs (total)                      :: 4,076
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 89
            rRNAs                             :: 10, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 10 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 19 of 69
            Pseudo Genes (incomplete)         :: 44 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 10 of 69
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6128
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13376389-rid12479423.denovo.214"
                     /strain="HB27"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="China: Hebei"
                     /collection_date="2017"
     gene            107..3286
                     /locus_tag="KRL67_003393"
     CDS             107..3286
                     /locus_tag="KRL67_003393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HBH1524234.1"
                     /translation="MKIDMIYKLIRRNTEPSRWSRGVKYYTNQLVDYIDIDIDDLQNN
                     LLTFRGGVNSEDNYSFYFNSITIDIKHFKIIENDCSCADCQSKGKSIFICKHIVAIVI
                     KSLKQIKNKKDVPPILIDILGVDKDKLSNTNSKASLDLSKQLLSLLDNTKRERVNLDV
                     LINVDSNKAIAEFKIGIDKMYILKNITEFAYSKLNGYELDFGKKFTYNPNSHYFADED
                     EKLVDIIEEYGTNLSYNFSSSHSKKFMEINASSLKQFLNAIKYKDFTLIFSKEEYHPK
                     ILNDTLPVRFDFKNIEDKIVLYSKDSLPVPLTKKNDVLLYDGNIYLLSHKKAHDYSKI
                     YQVLSKTKELKFDKEDSKDVLGLLVPRLKSISQEVHLDDNIKNNITKDFKSEFYFDMV
                     DGNICCDVKYIYDDEDKKFVLPNTQKEAIIENKLTSSQFTKENNHYVFKGTDHDLFTF
                     LDLQLESLKEFGDIYYSEKFKLKKIYNASSIQASINQTNQNYLEFNFNISDINPKEYK
                     DILKAFQEKRTFYKLKDGNFIDLSERETKDFFELVENLDIMDKSKNNKIHKNKALYIN
                     DVIRSKNLKFIDGKKELDSICEKFRNFDSINLEIPTNLNANLRDYQINGLNWFKVLDY
                     YKFGGILADEMGLGKTIQTIAFLLSLSNKKSLIVTPTSLIYNWKNEFEKFAPDIKVLL
                     IHGNKRDREKCFIELENFDVILTTYGTLRNDLEKYSEIKFDYCIIDEAQNIKNPVALV
                     TESVKSINAENKFALTGTPMENNLLELWSIFDFIMPGYLYSKAKFQELFINKEDNVKN
                     LKKLIKPFILRRSKKQVMKELPDKIEKNFFVELNKEQKKIYSVYSKDIQDKMKDKNLK
                     KDKIVIFSYLTKLRQLCLDPSIVVKDYNKKSSKIETCLEILRDSINENHKILLFSQFT
                     SVLKNISKELDKYKIKYHYIDGKTNAKERLELVDEFNNSMDKKVFLISLKAGGTGLNL
                     TSADMVIHFDPWWNPSVENQASDRAHRFGQKNSVQVIKLIAKGTIEEKIIKLQESKKE
                     LINQFINGELSNEGVLKSLSDEEIIDLFN"
     gene            complement(3571..4335)
                     /locus_tag="KRL67_003394"
     CDS             complement(3571..4335)
                     /locus_tag="KRL67_003394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBH1524235.1"
                     /translation="MAYFNYNDKQCYYNEIGDGTPLLFLHGNTASSNMFNDIIDFYKD
                     EYKVILIDFVGHGKSQMVDKFSADLWFDEAMQVICFLEKMNYKKVNIIGSSGGALVAL
                     NVALERPDLVNKVIADSFEGEVPLESFVQNVKIEREASKQDDGAKAFYIYNQGENWEK
                     VVDNDTEAIFEHYKTIGKFFHKPLETMQPEVLLTGSREDEFVSLISNDFFENTFSSLL
                     EKIKNGKMYLFDKGGHPAILSNGLDFSNVAKKFLEE"
     gene            complement(4573..6066)
                     /locus_tag="KRL67_003395"
     CDS             complement(4573..6066)
                     /locus_tag="KRL67_003395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HBH1524236.1"
                     /translation="MKYTFEKFKLNEKILKSLKSLEYNIPSRVQREVIPKLLKGQNLV
                     VKSKTGSGKTASFAIPLCENINVDYNNIQALIVVPTRELALQVKDEISDIGRLKKVRC
                     SAIFGKQPIKEQIAELKQRVHIVVATPGRILDHINKGTIKLENIQYLVIDEADKMFNK
                     GFVEQMEKILLNLPKEKIVGLFSATIDEEIKYICEKYMPDYSVINIEENEIDNNQRTR
                     QIDDKIIKANGREKYILLKELIYSENPKSVIIFCNTKEKVSKLYNKMSKDGFLIRELH
                     ADLSQERRIFVIKDFKKQKFNVLVSSDVASRGIHIDDISLVINYDVPQDKENYIHRIG
                     RTGRKGNSGKAITIVNEKDEKYIENIETYIGYKINELTDIEQSRITQGKILFEEYSKE
                     ILKEVSKRKKADKNSYKSKDIKLNTESEVVKLYLNAGKKKKIRVLDIVGAFSNIKGIT
                     NDDIGVIEVQDLCSYVDILNYKGDLILKKYKEIPIKKKMVKVKRDIR"
BASE COUNT         2289 a          862 c          714 g         2263 t
ORIGIN      
        1 taatagataa aagctattca tatctgcctt aattttagtc ttaaatttag agtgtaaatt
       61 gaatgcattt agattataat atatacagtg aggtaaggtg aataatatga aaatagatat
      121 gatatataaa ctaataagaa gaaatacaga accatcaaga tggtcaagag gtgtaaaata
      181 ttatactaat caactagttg attatataga catagatatt gatgacctac aaaataatct
      241 gttgacattt cgtggtggag tcaattctga agataattat agcttttatt ttaattcaat
      301 aactatagat ataaagcact ttaagataat tgaaaatgat tgtagttgtg ctgactgtca
      361 atcaaagggc aaaagtattt ttatatgtaa gcatatagtt gctattgtga taaaaagctt
      421 aaaacaaata aaaaataaaa aagatgttcc tcctattcta atagatatat taggtgtaga
      481 taaggataag ctttctaata caaattctaa agccagctta gatttaagca agcaactact
      541 ttctttgtta gacaatacta aaagagaaag ggttaattta gatgtattga taaatgtgga
      601 ttcaaataag gcaattgctg aatttaaaat aggaattgat aaaatgtaca ttttaaaaaa
      661 cattactgaa tttgcatatt caaaacttaa tggatatgaa ttagattttg ggaagaaatt
      721 tacttacaac cccaacagtc attattttgc tgatgaagat gagaagttag tagatattat
      781 agaagaatat gggacaaatc tttcatacaa cttttcttca tcacactcaa aaaaatttat
      841 ggagataaat gcaagttctt taaaacaatt tttaaatgct attaagtaca aggattttac
      901 ccttattttt tctaaagagg aatatcatcc aaagatatta aatgatactc tacctgtaag
      961 gtttgatttt aaaaatatag aagataaaat agtcttatat tctaaagatt cactacctgt
     1021 tccacttact aaaaaaaatg atgtactttt atatgatggc aatatctatt tattatcaca
     1081 taaaaaagct catgattatt caaaaatata tcaagtatta tcaaagacta aagagttaaa
     1141 atttgataaa gaggattcta aggatgtttt aggattgcta gttcctagat taaaatctat
     1201 atctcaagaa gttcatttag atgataacat aaaaaataat ataacaaaag attttaaatc
     1261 tgagttctac tttgatatgg ttgatggcaa catatgttgt gatgtaaagt atatatatga
     1321 tgatgaagat aaaaaatttg tgcttcccaa cacacaaaaa gaagctatta ttgaaaataa
     1381 actgacttct tcacaattta caaaggaaaa taatcactat gtgtttaaag gaactgacca
     1441 tgacctattt acatttttag atttacaatt agaatctcta aaggaatttg gagatatata
     1501 ttattctgag aagtttaaat tgaaaaaaat atataatgct tcatcaattc aagcttcaat
     1561 caatcagact aaccagaatt acctagaatt taattttaat atatctgata taaatccaaa
     1621 agagtacaag gatattttga aagcttttca agaaaaacgt actttttaca aacttaaaga
     1681 tggaaacttt atagatttat cagaaagaga aacaaaagat ttttttgaat tagttgaaaa
     1741 tctagacata atggataaat caaaaaataa taaaatccat aaaaataaag ctctatatat
     1801 aaatgatgta atacgtagta aaaatttaaa gtttatagat ggtaaaaaag aactagacag
     1861 tatatgtgaa aaatttagaa actttgatag cataaattta gaaattccca caaatttaaa
     1921 tgcaaatctt cgtgactatc aaataaatgg tctcaactgg tttaaagttt tagattatta
     1981 taaatttggt ggtatattag ctgatgaaat gggtcttggt aaaacaatac agactattgc
     2041 atttttatta tcattatcca ataaaaaaag tcttattgtt acaccaacat cgctcattta
     2101 taattggaag aatgaatttg aaaagtttgc tccagatata aaagttttat tgatacatgg
     2161 aaataaaaga gatagagaaa aatgttttat agaattagaa aattttgatg taatacttac
     2221 aacatatggt actcttagaa atgatttaga aaaatatagt gaaataaagt ttgattattg
     2281 tataatagat gaagctcaaa atataaaaaa tccagtagct ttagtaactg agtctgtaaa
     2341 aagtataaat gcagaaaata aatttgcact cactggtaca cctatggaaa ataatctact
     2401 agaattatgg tctatatttg attttataat gcctggctat ctatatagta aagcaaaatt
     2461 tcaggaattg tttataaata aagaagataa tgttaaaaac ctgaaaaaat tgataaaacc
     2521 ttttattctt agaagaagta aaaaacaagt tatgaaagaa cttccagata agattgaaaa
     2581 aaactttttt gttgaattga ataaagagca gaaaaagata tatagtgtat attcaaaaga
     2641 tatacaagat aaaatgaaag ataaaaatct aaagaaagat aaaatagtta tattttctta
     2701 tttaactaaa ctaagacaac tttgtttaga cccaagtata gtagtaaaag attacaataa
     2761 aaaaagttca aaaattgaaa cgtgtttaga aatacttagg gatagtataa acgaaaacca
     2821 caaaatatta ttgttttcac aatttacttc tgtgcttaag aacatcagta aggaacttga
     2881 taagtataaa attaaatacc attatataga tggtaaaact aatgccaaag aaagacttga
     2941 acttgtagat gagttcaata atagtatgga taaaaaagtt tttttaatat ctttaaaagc
     3001 aggcggaact ggtttaaatc ttacatctgc tgacatggta attcactttg acccatggtg
     3061 gaatccatca gtagaaaatc aagctagtga tagagctcat agatttggtc aaaaaaattc
     3121 agtacaagtt ataaaattga ttgctaaagg gactatagaa gaaaaaataa taaaattaca
     3181 agaaagtaaa aaagagctta taaatcaatt tataaatgga gaactttcta atgaaggcgt
     3241 attaaaaagt ctctctgatg aggagataat agacttattt aactaaaata tttaatccta
     3301 cagcttaact atataaattt gttctaacat ttatatagtt aagctacaat acctgaataa
     3361 ataaaatttc atattgttat ttaatcccaa ctttacaatc caatgatatc tctttctata
     3421 catattgcat ataaaaatgt aataacttga ctattagaat attttgtttt aacaatccta
     3481 tttcatcata ataatgaagt atatgtacac tcacctttgt aagttttgca atccatttta
     3541 cagttttcat tattgtaaat tttggttaaa ttattcctct aaaaatttct ttgctacatt
     3601 tgaaaaatct aatccattcg ataaaattgc aggatgacca cctttatcaa acaaatacat
     3661 tttaccattt ttaatctttt caagaagtga tgaaaaagta ttttcaaaaa aatcattaga
     3721 tattagagat acaaactcat cttctctact tcctgttaaa agcacctctg gttgcatagt
     3781 ttctagaggc ttatgaaaaa attttcctat agttttataa tgttcaaaaa tagcttctgt
     3841 atcattatct acaacttttt cccagttttc tccttgatta taaatataaa aagcttttgc
     3901 accatcatct tgctttgatg cctcacgctc tattttaaca ttttgtacaa atgactctaa
     3961 aggaacttct ccttcaaaac tatctgcaat tactttatta actaaatcag gtctttcaag
     4021 tgcaacattt aatgcaacaa gagctcctcc actactacca attatattta cttttttata
     4081 attcattttt tctaaaaaac atatcacttg catagcttca tcaaaccata agtcagcaga
     4141 aaacttatcg accatttgag attttccatg acctacaaaa tcaattaata ttactttata
     4201 ttcatcttta taaaaatcga ttatatcatt aaacatatta gaagaggctg tatttccatg
     4261 taaaaaaagc aatggtgtac catccccaat ttcattatag tagcactgtt tgtcgttata
     4321 gttaaaatat gccatatatt tcccccttta atattcctat aagtatatat ttattactta
     4381 tatttaaaat gactattgtc ataaacactt aaaaatagta tagcttagaa tgtagctaaa
     4441 gtcaacattc ctttccctaa caacttctat tgtttttttg caacttcatt tcgataacag
     4501 aataattcat caatattgat acttttactt aaatatatga tttcatcatc agagagagca
     4561 ataataacta aattatcgta tatctctttt cactttaacc atttttttct ttataggtat
     4621 ctccttatat ttttttaata tcaaatctcc cttataattt aatatatcta catatgaaca
     4681 taaatcttga acttcaatta cacctatatc atcattagtt atacctttta tattactaaa
     4741 tgcaccaact atatctaaaa ctcttatttt cttcttctta ccagcattta aatataattt
     4801 aacaacttca ctttctgtat ttagttttat atctttactt ttataagaat ttttatcagc
     4861 tttttttctc tttgatactt cttttaggat ttcttttgaa tattcttcaa acaaaatttt
     4921 gccctgcgta attctacttt gctctatatc tgttagctca tttattttat atcctatata
     4981 cgtttctata ttctctatat acttttcatc cttttcattt actatagtaa ttgcctttcc
     5041 actatttcct tttctcccag ttctacctat tctatgtata tagttttctt tatcttgtgg
     5101 cacatcataa tttattacta aagaaatatc atcaatatgt attcctctag acgctacatc
     5161 actactaact aatacattaa acttttgttt tttaaagtcc ttaatcacaa aaattcttct
     5221 ctcctgcgat aaatctgcat gtaactctct tattaaaaaa ccgtctttac tcatcttatt
     5281 atataattta gatactttct ctttagtatt acaaaatatt atcacactct ttggattttc
     5341 gctgtatatc aattctttta aaaggatata cttctctctt ccatttgcct tgattatttt
     5401 gtcatctatt tgtcttgttc tttgattatt atcgatctca ttttcctcta tatttataac
     5461 actgtaatct ggcatatact tttcacatat atattttatc tcttcatcta ttgtagctga
     5521 aaaaagacct actattttct ccttaggtaa atttaataat attttttcca tttgttcaac
     5581 aaaaccttta ttaaacattt tatctgcctc atctattact aaatactgta tattttccaa
     5641 cttaatagta cctttattta tatggtcaag tattctgcct ggagtagcta caacaatatg
     5701 aaccctttgt tttaattcag ctatttgctc ttttataggt tgtttgccaa atatagcact
     5761 acatctcacc ttctttagtc ttcctatatc cgatatctca tcttttactt gcaaggctaa
     5821 ttctcttgtt ggaaccacta taagagcttg aatattattg taatctacat ttatattctc
     5881 acataaaggt attgcaaaac ttgctgtctt tccacttcct gtttttgact taactactaa
     5941 gttttgacct tttaataatt ttggaataac ttctctttgt actctagaag gtatattata
     6001 ttctaaactc ttcaacgact taagtatctt ttcatttaac ttaaattttt caaatgtata
     6061 tttcatatat taccacctta ttgtatttat cttatctata tatttttcta cattatatta
     6121 ttataata
//
LOCUS       DAEPXF010000207         6116 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain HB27
            SAMN13376389-rid12479423.denovo.215, whole genome shotgun sequence.
ACCESSION   DAEPXF010000207 DAEPXF010000000
VERSION     DAEPXF010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13376389
            Sequence Read Archive: SRR10518432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6116)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6116)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001069624.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001069624.1
            Genome Coverage       :: 315x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/15/2021 01:54:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,165
            CDSs (total)                      :: 4,076
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 89
            rRNAs                             :: 10, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 10 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 19 of 69
            Pseudo Genes (incomplete)         :: 44 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 10 of 69
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6116
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13376389-rid12479423.denovo.215"
                     /strain="HB27"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="China: Hebei"
                     /collection_date="2017"
     gene            68..799
                     /locus_tag="KRL67_003396"
     CDS             68..799
                     /locus_tag="KRL67_003396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HBH1524237.1"
                     /translation="MNIYAHRGFSGKYPENTILAFKKCLDMDIYGIELDVHRTKDGKI
                     VVIHDEKVDRTFNGHGFVKDFTLRKLKTLNSSFEGYQNNKECKIPTLEEVLILVSPTD
                     LILNIELKTDKVNYPNIEKDVLELILKYNMKNRVLISSFNSNSLKNFHKLDSSVKTGL
                     LCYLPINNVVNFAKFLGNSYLHPPLVLVNESLIELCHKNLLGVNVYTVNEEDDIIHCL
                     KLNVDGIFTNYPDIASNLLHSKQYS"
     gene            879..1514
                     /locus_tag="KRL67_003397"
     CDS             879..1514
                     /locus_tag="KRL67_003397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HBH1524238.1"
                     /translation="MDNLLNELEALKSNLDNLPIEKIENYDLSKTALFIIDVNNGFAR
                     QGALYSPRVESLIKPIEMFTKKISNKLNRVIAFTDSHTPKSIELLSYPVHCLENDVES
                     ELVDELKSIENLQILPKNSTNGFFALENLDFDNIDNIIIVGDCTDICIYQFAITLKSY
                     FNQHNIEKNIVVPMNLVDTYDIPNVHPAEILNLVFFNSMIQNGVNVLKEIR"
     gene            complement(1765..2358)
                     /locus_tag="KRL67_003398"
     CDS             complement(1765..2358)
                     /locus_tag="KRL67_003398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3867 domain-containing protein"
                     /protein_id="HBH1524239.1"
                     /translation="MSDDRIIDFNELKNKVKDSDVDKFEQYIYNLYFSVMDGKMSMAE
                     FSRKIFDYMRDNNISQEKFMKIQKQFMERYGMDTEEVEKQLRNFGIDPSTAGFMSNNT
                     SSKVSTEDLESFKKSAGFYEKYGEKIQPKSCITTFIKNDLNDINVIIDQEKIMLCSDR
                     KINLMDSELNEFLLEYKNMFNKKIKVVMCETTNKYDY"
     gene            complement(2429..3292)
                     /locus_tag="KRL67_003399"
     CDS             complement(2429..3292)
                     /locus_tag="KRL67_003399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HBH1524240.1"
                     /translation="MLGIIAPSGPLRNTSLEEIKFNLESYGYEVKFSESCSLNYKGYL
                     AGNDDIRARDIEDMFLDKDVDIIMCLRGGYGTTRILDKINYDIIKQNPKPFIGFSDIT
                     GLNLAFYKNCGLLPYHGIMAANVGKWDEFTYKSLVNALEFKDDLYLENPKEENIYTVC
                     EGKAEGIIMGGNLSLIIATMGTKYEIDAKDKILFIEEIGEPQYKLDRMLTQLYSSGKL
                     EECNGIIFGDFRDCIEENDLIELLIEFANKVNKPSIYNLQSGHCIPMITIPLGRMCEL
                     DATEKIVKLKK"
     gene            3455..4624
                     /locus_tag="KRL67_003400"
     CDS             3455..4624
                     /locus_tag="KRL67_003400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HBH1524241.1"
                     /translation="MLNIRTLCHEINDWVINIRRDLHKTPELGLEEFQTKKKIIKYLN
                     EIGINYIEYKNHTGITAYINVNPNFETVAIRADIDALPITEELNYSYKSINIGKMHAC
                     GHDAHTAILLGTCNILFKLKDYLNVNVKFFFQPAEETIGGAQLMIEDGCLENPNVKYI
                     FGLHVNPNINKNLIELKYNTLNASTDTLQLTVHGSKCHGAYPHQGIDAIVISAHIITA
                     LQTIVSRNTNPTDSVVISLGKIEGGIKENIVCDKVVIRGTLRTLTPETREFSKKRIRE
                     ICDFTCKTFGGSISVEIEEGYPALINSNHLVDYVKQNAVELFGEENIILKDSPTLGAE
                     DFSYFLRHCEGAFYHLGCANREKNITSPLHTSTFDIDEDCLITGVMLHVKNILSF"
     gene            4725..5201
                     /locus_tag="KRL67_003401"
     CDS             4725..5201
                     /locus_tag="KRL67_003401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HBH1524242.1"
                     /translation="MKEILINPIGKIRIQGEEVFIKLDKKYVPALNELDDFSHLNVFW
                     WADGFDNPELRSILETPKPYKSGPDVIGIFATRSPIRPNPIALTTVQILNIDHENGII
                     KIPYIDANDNSPVIDLKPYTPSVDRVENPSVPKWCSHWPKSVEKSGEFNWEDEIEF"
     gene            complement(5606..>6116)
                     /locus_tag="KRL67_003402"
     CDS             complement(5606..>6116)
                     /locus_tag="KRL67_003402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBH1524243.1"
                     /translation="KYLKKSLNKLAKLQRELSRKTIGSLNRNKARLKVARLQEHIANQ
                     RNDFLQKLSTKLIKENDIICIEDLQVKNMIRNRKLSRLISDVSWSEFIRQLEYKANWY
                     GRKIVKVGKFFASSQICNKCGYKNEEVKDLNIREWICPSCNETHDRDINASINILKEG
                     LRLITIQNK"
BASE COUNT         2143 a          909 c          754 g         2310 t
ORIGIN      
        1 aatatgatat attatattaa ttaaattttt ataagataag ttatatttat cgaaaggggc
       61 cacataaatg aatatttatg ctcatagggg gtttagtgga aagtatccag aaaatactat
      121 tctagctttt aaaaaatgtt tggatatgga tatatatgga atagaacttg atgtccacag
      181 aactaaggat ggaaaaattg tagttattca tgatgaaaag gttgatagaa cttttaatgg
      241 acatggtttt gtaaaagact ttactttaag aaaattaaaa actctaaact catcttttga
      301 aggatatcaa aacaataaag aatgtaaaat tccaacttta gaagaagtat taattctagt
      361 ttcacctaca gaccttattt taaatataga gctaaaaact gacaaagtaa actatcccaa
      421 tatagaaaaa gatgttttag aacttatttt aaagtataat atgaaaaata gagttcttat
      481 atctagtttc aacagcaata gtttaaaaaa ctttcataaa ttagactcta gtgtaaaaac
      541 aggattattg tgttatttac ctataaacaa tgtagtcaat tttgctaaat ttctgggtaa
      601 tagttacctt catccacctc tagttttagt aaatgaatct ctaattgaac tttgtcacaa
      661 aaatctactt ggtgtcaatg tatatactgt aaatgaagag gatgatataa tacactgtct
      721 aaaactcaat gtagatggta tttttactaa ctatccagat attgcttcaa atcttttaca
      781 ctcaaaacaa tatagctaat tacacaatag tttgtactcc ttgactttta tataattaat
      841 atgtacaatt ttattaaatt tatcttggag gtataactat ggataactta ttaaatgaac
      901 ttgaagcttt aaagagtaat ttagataatc taccaataga aaaaatagaa aattatgatt
      961 tgagtaaaac tgctttattt ataattgatg ttaataatgg ctttgcaaga caaggtgcat
     1021 tatactctcc tagagtagag tctttaataa agccaattga aatgtttaca aaaaaaatat
     1081 caaacaaatt aaatagagta atagcattta ctgattctca tactcctaaa tcaatagaac
     1141 ttttgagtta tcctgttcac tgtttggaaa atgatgtaga aagtgaattg gttgatgaac
     1201 ttaagtcgat agaaaaccta caaattcttc ctaaaaattc tactaatgga ttttttgcat
     1261 tagaaaattt ggattttgat aatatagata atataattat agttggcgat tgcacagata
     1321 tatgtatata tcaatttgca ataactctaa aatcatattt taatcaacat aatattgaaa
     1381 aaaatatagt tgtacctatg aatttagtag atacttatga tattcctaac gttcaccctg
     1441 ctgagatttt aaacttagta tttttcaaca gtatgattca aaatggagtt aatgtattaa
     1501 aagagattcg ttaatcctct aatactttat atcccatata atacaattat catatattta
     1561 tttttaataa aatctctaat agaaaaacac agttttaatg agaagattac aatttagaac
     1621 attatctatt atgactaatc ttcacaatat aaaaaatcgc agtttagata ttccttatac
     1681 aaatttataa ggaataataa attgcgattt ttatttttaa gtaatacgat taatcgatac
     1741 tacttcagta tttaaattca tttcttaata gtcatactta ttcgtagttt cgcacattac
     1801 tacctttatt tttttattaa acatattctt atattctaat aaaaactcat ttagttctga
     1861 atccataaga tttatttttc tatcactaca aagcattatc ttttcttggt ctattattac
     1921 attaatatca tttaagtcat tttttataaa tgttgttata caactctttg gttggatttt
     1981 ttcgccatat ttctcataaa atcctgcact cttcttaaat gactctaaat cttctgtaga
     2041 tactttgctg gaagtattat tgctcataaa acctgctgta gaagggtcga ttccaaagtt
     2101 tctaagttgt ttttctactt cttcagtgtc cataccatat ctctccataa attgcttttg
     2161 tattttcata aatttttctt gagatatgtt gttatctctc atataatcga atatttttct
     2221 tgaaaactca gccatagaca tctttccatc cataacagaa aaatataagt tatatatgta
     2281 ttgctcaaat ttatctacat cagaatcttt aactttgttc tttagttcgt taaagtctat
     2341 tattctatca tcagacattt ttcatcctcc tagctaaatt tttacttgta taagattata
     2401 ccatatttct attatattta tatacacact atttttttaa tttaacgatt ttttctgtag
     2461 catctaattc gcacattctg cccaaaggaa tagttatcat tgggatacaa tgacctgact
     2521 gaagattata tatagaaggt ttattgacct tatttgcaaa ttctattagt aattctatta
     2581 aatcattttc ttcaatacaa tccctaaaat ctccaaaaat tataccatta cattcctcaa
     2641 gttttcctga agaatatagt tgtgttaaca ttctatccag tttatattga ggttctccaa
     2701 tttcctcaat aaacaaaatt ttatcttttg catctatttc atattttgtt cccattgtgg
     2761 caattattaa ggataaattt cctcccatta ttattccctc tgctttacct tcacatacag
     2821 tataaatatt ttcttctttt ggattttcta aatataagtc atctttaaat tctaaagcat
     2881 ttaccaatga tttataggta aattcatccc atttacctac attcgctgcc attattccat
     2941 gatatggtaa aaggccacaa tttttataaa aagctaaatt taaacctgtt atatcagaaa
     3001 aacctataaa tggttttgga ttttgtttta ttatatcata atttatttta tccaatattc
     3061 ttgttgttcc ataacctcct cttaagcaca taattatatc tacatcttta tctaaaaaca
     3121 tatcttctat atctcttgct ctaatatcat cattaccagc taaataacct ttataattta
     3181 aactacaact ttcagaaaac ttaacttcgt aaccataact ttctaaatta aactttattt
     3241 cctccaaact tgtatttctt aatggcccag atggcgcaat tattcctaac atttaaacac
     3301 ctcttcttat aaaatgatat attcttctcc atactaaata tctctaatat aattataaga
     3361 tattttattt gtacttgata cataataatg atttacataa aataatataa ttaatgaaga
     3421 aaaataaatt tttaatatag ttaggatgtg ttttatgctt aatataagaa ctctttgtca
     3481 tgagataaat gattgggtta taaatataag aagagattta cataagactc cagaacttgg
     3541 attagaagaa tttcaaacta agaaaaaaat tattaaatac ttaaatgaaa taggtataaa
     3601 ttatattgaa tataagaatc atacagggat aactgcatac attaacgtga atccaaactt
     3661 tgaaacagtt gctataagag ctgatataga tgccttacct ataacagaag aacttaatta
     3721 ttcttataaa tctattaata ttggtaaaat gcatgcatgt ggccatgatg cacatactgc
     3781 tattttactg ggtacttgta atatactatt taaattaaag gattacttaa atgtaaacgt
     3841 aaaattcttt tttcaacctg ccgaggaaac tattggtggg gctcaactta tgatagaaga
     3901 tggatgtctt gaaaacccaa atgtaaaata tatatttgga cttcatgtaa atcccaatat
     3961 caacaaaaat ctaattgaat taaaatacaa cactctaaat gcttccactg atacacttca
     4021 attaactgta catggttcaa aatgtcatgg agcttatcca catcaaggta ttgatgctat
     4081 tgttatatct gctcatatta ttacagctct acagacaatc gttagtcgaa acacaaatcc
     4141 aactgattct gtggttattt ctctaggaaa gattgaaggt ggaatcaagg aaaatatagt
     4201 ttgtgataaa gtagttataa ggggtacttt aagaaccttg actcctgaaa ctagagagtt
     4261 ttcaaaaaaa cgaattagag aaatttgcga ttttacttgt aaaacatttg gaggaagtat
     4321 ttctgttgaa attgaggaag gttatccagc tcttatcaat tcaaaccatc tagtagatta
     4381 cgtcaaacaa aatgctgtag aattgtttgg agaagaaaat attatcctta aagactcgcc
     4441 cactcttggg gctgaagact tttcgtattt tctaagacac tgtgaaggag ctttttacca
     4501 ccttggttgt gcaaatcgtg aaaaaaacat tacttcccct ttgcatacct ctacttttga
     4561 tattgacgaa gattgcctta ttactggtgt catgctccat gtaaaaaata tattatcatt
     4621 ctaactatta atatttttat ttattatttt atattgaccc tcacatgatg tcatgttata
     4681 ccctaaaatt acaaatgata aattaaatag ttggaggtaa tattatgaaa gaaatattaa
     4741 taaatcctat aggtaaaata cgtattcaag gcgaagaggt ctttattaaa ttagacaaaa
     4801 agtatgtacc tgcattaaat gaactcgacg atttcagtca tttaaatgta ttttggtggg
     4861 ctgatgggtt tgataatcct gaactaagaa gtattttaga aaccccaaaa ccttataaaa
     4921 gtggaccaga tgttatagga atatttgcaa caaggtcacc tataagaccc aacccaatag
     4981 cattgactac tgtacaaatt ctcaatatag accatgaaaa tggtataata aaaataccat
     5041 atattgatgc taatgataat agtcctgtta ttgacttaaa gccatatact ccaagcgttg
     5101 atagggttga aaatccgtca gttcctaaat ggtgtagtca ctggcctaag agtgtagaaa
     5161 aatcaggaga atttaattgg gaagatgaaa ttgaatttta aattactttt ttcttgattt
     5221 cattaactcg ttttccacat ttattttatt tacacttacc aatattttat gtcacatatg
     5281 catataatat taggctgtat tatataatac agcctaatat tatataataa atttctattt
     5341 ttcccttaaa ccatccacat tctttctaag ctacataaga tttttctatt atttcttttt
     5401 gagtttaatt tagcattatg ttgaggaaaa aacatttcat gcatttttaa taaattaaat
     5461 ttttctttta ttctacgatt ttataatact gaacctccca tgactaaagt cacagggttc
     5521 ctgcttcata gaattttgca tactaaaata tgtcttacta attctccaca ggctatcccc
     5581 gtaattccta cggttcttac ataagttatt tattttgaat tgttattagt cttaatcctt
     5641 cttttagtat atttatactt gcatttatat ctctatcgtg agtttcatta caactaggac
     5701 aaatccattc tcttatattt aagtccttaa cttcctcgtt tttataccca catttattgc
     5761 atatttgtga acttgcaaag aatttaccta cttttacaat ttttctacca taccaattag
     5821 ccttgtattc taattgacga ataaattctg accatgatac atctgaaatt aagcgagata
     5881 gtttacgatt tctaatcata ttctttactt gtaaatcttc tatgcaaata atatcattct
     5941 cttttattaa tttagtagat agtttttgta gaaaatcatt tctttgatta gctatatgtt
     6001 cttgaagtct tgcaactttt aatcttgctt tatttctatt taaactaccg attgtttttc
     6061 tcgataattc tctttgtaat ttagcaagtt tgtttaatga cttcttaaga tactta
//
LOCUS       DAEPXF010000208         6096 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain HB27
            SAMN13376389-rid12479423.denovo.216, whole genome shotgun sequence.
ACCESSION   DAEPXF010000208 DAEPXF010000000
VERSION     DAEPXF010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13376389
            Sequence Read Archive: SRR10518432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6096)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6096)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001069624.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001069624.1
            Genome Coverage       :: 315x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/15/2021 01:54:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,165
            CDSs (total)                      :: 4,076
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 89
            rRNAs                             :: 10, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 10 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 19 of 69
            Pseudo Genes (incomplete)         :: 44 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 10 of 69
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6096
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13376389-rid12479423.denovo.216"
                     /strain="HB27"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="China: Hebei"
                     /collection_date="2017"
     gene            195..1202
                     /gene="asrA"
                     /locus_tag="KRL67_003403"
     CDS             195..1202
                     /gene="asrA"
                     /locus_tag="KRL67_003403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfite reductase subunit AsrA"
                     /protein_id="HBH1524244.1"
                     /translation="MKLKLDSSKFNEGLNYLKKDYKIFAPVLIPFKGTFSDTDVIRYK
                     EVNTFEEMEFAKKANFSPKEVVLPINQVLFYFTEKEFKESDLENKKILIFLRACDING
                     IKRLDEIYLNNGEEKDYFYKNIRDKVKFALVGCKESFRNCFCVSMDSNKTDNYSIGLN
                     IEGDTLYLDIKDSEFEVFNGEATEFNVDYVTDNLISVDVPENIDSNEMIGNSIWDEYD
                     TRCIGCGRCNFVCPTCSCFTMQDIFYKENENVGERRRVWASCQVDGYTDIAGGNSFRK
                     KQGERMRFKTMHKIHDFKKRFGYHMCVGCGRCDDACPQYISFSKCIEKINDLVTSKEE
                     V"
     gene            1203..1994
                     /gene="asrB"
                     /locus_tag="KRL67_003404"
     CDS             1203..1994
                     /gene="asrB"
                     /locus_tag="KRL67_003404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfite reductase subunit AsrB"
                     /protein_id="HBH1524245.1"
                     /translation="MNPYIPVSAKILDIVKHTDKEWTFRVNCDTKGVLPGKFYEISIP
                     KYGESPISVSGYGEDYIDFTIRNVGKVTSELFNYKEGDSFFIRGPYGNGFDVSLYEGR
                     EIVVVAGGSALAPVRGIVEHFYNNKEKCESFKLIVGFKSPKDVLFTDDLKRWSEKLDI
                     LVTVDGADEGYSGNVGLVTKYIPELDIKDINNTSIIVVGPPMMMKFTVEEFLKRDLDE
                     KNIWVSYERKMCCGIGKCGHCKMDDTYICLDGPVFDYSYAKNLID"
     gene            2007..2978
                     /gene="asrC"
                     /locus_tag="KRL67_003405"
     CDS             2007..2978
                     /gene="asrC"
                     /locus_tag="KRL67_003405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite reductase subunit C"
                     /protein_id="HBH1524246.1"
                     /translation="MIRDLNTKKVMKNAYRITKTKYETSLRVRVPGGLIDPESLAIVS
                     KIASEYGNGQVHITTRQGFEILGINMEDMEEINKIIQPVIEKMNINQSDKNAGYPAAG
                     TRNIAACIGNKVCPKAQYNTTEFAKRIEKAIFPNDLHFKVALTGCPNDCIKARMNDFG
                     IIGMALPIYEKDRCVNCGACVKKCSKISVGALKTENNKVVRDKDKCIGCGECVLNCPT
                     NAWTRDEKKYYRLAIMGRTGKKNPRLAEDFLLWVDEDSIIKIILNTYRYVEKYIDKDA
                     PGGKEHIGYIIDRTGFMEFKKWALEGVELPEITKMYENIYWSGIKYL"
     gene            3035..3811
                     /locus_tag="KRL67_003406"
     CDS             3035..3811
                     /locus_tag="KRL67_003406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HBH1524247.1"
                     /translation="MHKETLDKLTNAAINKINLLNTSKIKYLVSSAFAGLYVGIGILL
                     IFTIGGLLTDAGSPMTKIVMGLSFAIALSLVIMTGTELFTGNNMVMSAGMLNKGVSIK
                     DTSKIWAYSWVGNLIGALILGLIFVGTGLVDKGPVAEFFANTAASKASMPFTALFFRG
                     ILCNILVCVSVLCSFRTNSDTAKIIMIFLCLFAFITSGFEHSVANMTIYSVSLFSPAI
                     STVTIGGAIYNLVAVTLGNIVGGALFMGLGTYILGKEKLN"
     gene            4082..>6096
                     /locus_tag="KRL67_003407"
     CDS             4082..>6096
                     /locus_tag="KRL67_003407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HBH1524248.1"
                     /translation="MTKKLVISKTKLFIFIIAFISIFIYLFGSKNTLIGVGIVTAMLT
                     LLERDLTISPIKNLLKYLAINIILGILSFFAVQNMYLGVLLNFIALFIIGYVFSYDLK
                     RAVYVPFGLMYIFMISIPVGKSEFPMRLLALAAGAVIIMIAQFVMNRNRMKNVGDKEL
                     ISICDELLEKISLLKNTINDDSSIIKSSMRKVDSCNYRINSISKNLKMVIFDNRKDDF
                     YISIRGIDIMNILFSLERINLILERYKKDSKEFEDEDIKNILEESSINSKSDVLVVAT
                     KEVNYIKLCLENKDTITDKEILGFRDYVIAQDTKNINLKEIYSVLENLYEFLVDYKKV
                     KSRDEKKAERKSKIPREFKRLSIYKKNFNLNSIRFSYAVKIALATAVAGFIMDYFHLK
                     DGRWIMLTVFSLTQPYAENCIQRSRKRIEGTFIGAVIFIVLFSIIKDSTLRSLIVLLA
                     GYINSYVVDYRKLMVCVTVSALGSAVVMGDPNVLTISRISYVALGAIIALIVNKFILP
                     YDAKTGYQHVIEMYKGIVKNIIDEVNKSIENVADVYYIKNLLLIPSLIEDRLMLINAI
                     YKDKHQEDFLENQKLLISNMYNLYINVNKNKIKDEDVEKILRDTNYISNYNVDKYDEG
                     RNVILESIVNTKSLGDKIICLNLLQTLNGVKEMYRISNITKVSIEEAA"
BASE COUNT         2460 a          545 c         1112 g         1979 t
ORIGIN      
        1 atttaagtga ttggatttga aatcgaataa tatatataat aaaaaaatat taacagagat
       61 taataaactc cattaatatt ttttttatta tttttgaaac tgtgataatt atcacggaag
      121 catagaaaat aaagtgttaa gatttaaaca agatagcaag taagtaaaac taaaatataa
      181 gaaaggtgat aaaaatgaaa ctaaaattag attcatctaa gttcaatgaa ggtctgaatt
      241 atctaaaaaa agactacaaa atatttgcac cagttttaat accatttaaa ggtacttttt
      301 cagatactga tgttataaga tacaaggagg taaacacttt tgaagaaatg gaatttgcta
      361 agaaagcaaa tttttcacca aaagaagttg tacttcccat aaatcaagta ttgttttatt
      421 ttactgaaaa agagtttaag gaaagcgatt tagagaacaa gaaaatttta attttcttaa
      481 gagcttgtga cataaatggt ataaaaagac ttgacgaaat atatctaaat aatggcgaag
      541 aaaaagatta tttttataaa aatatcaggg ataaagtaaa gtttgcactt gtagggtgta
      601 aggaaagttt tagaaactgt ttttgtgtga gtatggattc taacaaaact gataattatt
      661 caattggact aaatatagaa ggagatacac tttatttaga tataaaggat agtgaatttg
      721 aagtatttaa tggagaagca actgaattta atgttgatta tgtaactgat aatttaatat
      781 ctgtagatgt gccagagaat attgattcaa atgaaatgat aggaaattca atatgggatg
      841 aatatgatac aagatgtata ggatgcggta gatgtaattt tgtatgtcca acatgttcat
      901 gttttacaat gcaggatatt ttctataagg aaaacgaaaa tgtaggtgag agaagaagag
      961 tttgggcttc ttgtcaagta gatggatata cagatatagc aggtggaaat tcttttagaa
     1021 aaaaacaagg tgaaagaatg agatttaaaa ctatgcataa aatacatgat tttaaaaaaa
     1081 gattcggata tcatatgtgt gttggttgtg gaagatgtga tgatgcctgt ccacaataca
     1141 tatcattttc aaagtgtata gaaaaaataa atgatttagt cactagtaag gaggaagtat
     1201 aaatgaaccc atatatacca gtaagtgcaa aaatactaga tatagttaaa catactgata
     1261 aagaatggac ttttagagta aactgcgata caaaaggtgt attgcctgga aagttttatg
     1321 agatatctat acctaaatac ggtgaaagtc caatatcagt atcaggatat ggagaggatt
     1381 atattgactt tacaataaga aatgtaggaa aagtaactag cgagttgttt aattataaag
     1441 aaggagattc tttttttata agaggtcctt atggaaatgg atttgatgta tctctttatg
     1501 aaggaagaga aattgtagta gtagcaggag gaagtgcttt agcccctgta agaggaatag
     1561 tagaacattt ttataataat aaagaaaaat gtgaaagttt taaattaata gtaggattta
     1621 aatcaccaaa agatgtttta tttacagacg atttaaaaag atggagtgaa aaacttgata
     1681 ttttagttac tgtagatgga gcagatgaag gatatagtgg aaacgtagga ttagtaacaa
     1741 aatacatacc agaattggat ataaaagata taaataatac ttctataatt gtagttggac
     1801 ctccaatgat gatgaaattt acagtagaag aatttttaaa aagagattta gatgaaaaga
     1861 acatttgggt ttcttatgaa agaaaaatgt gttgtggaat aggtaaatgt ggacattgca
     1921 aaatggatga tacttatata tgtttagatg gaccagtatt tgattattca tatgctaaaa
     1981 atcttattga ttagggggcg aagtttgtga taagagattt aaatactaaa aaagtaatga
     2041 aaaatgctta tagaattaca aaaacaaaat atgagacttc tttaagagtt agagtacctg
     2101 gtggtcttat tgaccctgag agtttagcta tagtttctaa aattgcaagt gagtacggca
     2161 atggacaggt acacataact acaagacagg ggtttgaaat attaggaata aatatggaag
     2221 atatggaaga aataaataaa attatacaac ctgttattga aaaaatgaac ataaatcaaa
     2281 gtgataaaaa tgcaggttat ccagcagcag gtacaagaaa tatagcagcg tgtataggaa
     2341 ataaagtctg tccaaaggct caatataata caactgaatt tgctaaaaga atagaaaaag
     2401 ctatatttcc aaatgattta cattttaaag tggctttaac tggttgtcca aatgattgta
     2461 taaaagctag aatgaatgac tttggaatta ttggtatggc tttaccaata tatgaaaaag
     2521 atagatgtgt aaattgtgga gcatgtgtaa aaaaatgttc taaaatatct gttggagcat
     2581 taaaaacaga aaataataag gtggtaagag ataaagataa gtgtatcggc tgtggagagt
     2641 gtgtactaaa ttgcccaaca aatgcatgga ctagagatga gaaaaaatac tatagattag
     2701 ctataatggg tagaacagga aagaaaaatc ctagactagc agaagatttt cttttatggg
     2761 tagatgagga cagcataata aagataatac ttaatactta tagatacgta gaaaagtata
     2821 tagataagga tgcaccagga ggaaaagaac atataggata tataattgat agaacaggat
     2881 ttatggaatt taaaaaatgg gcacttgaag gtgtagagct tccagaaata actaaaatgt
     2941 acgaaaacat atactggagt gggattaaat atctataaat tgagtaaaac atatatttga
     3001 ataaattaaa atttaataga aggaagaggg aattatgcat aaagaaactt tagataaatt
     3061 aactaatgca gctataaata agataaattt attaaacaca agtaaaataa aatatttggt
     3121 atcttctgcg tttgcaggac tttatgtagg tataggtata cttttgatat ttactattgg
     3181 aggacttttg actgatgcag gttcacctat gactaagata gtaatggggt tatcatttgc
     3241 aatagcacta agtttggtta taatgacggg aacagagtta tttactggaa acaatatggt
     3301 tatgtctgct ggtatgttaa ataaaggtgt aagtataaaa gatacttcta aaatatgggc
     3361 atacagttgg gtaggtaatt taatcggagc tttgatttta ggtcttatat ttgtaggaac
     3421 tggtctagta gacaaaggcc ctgtagctga attttttgca aatacagcag ctagtaaagc
     3481 atccatgcca tttacagctc ttttctttag aggaatttta tgtaatattc tagtatgtgt
     3541 atcagtgttg tgttcattta gaactaatag tgatactgca aaaataatta tgatattctt
     3601 atgtttattt gcttttataa catcaggttt tgagcatagt gtagcaaata tgacaattta
     3661 tagtgtttca ttattttcac cagcaataag tacagttact attggaggag ctatttataa
     3721 tttagtagct gtaacacttg gaaatattgt tggaggagct ttatttatgg gtcttggaac
     3781 ttatatattg ggtaaagaaa agcttaatta aatttattgt ataaattata actctcttaa
     3841 taaatacatt tttagtattg ttgagagagt tatttttata tttttgaaaa aatatttgaa
     3901 agaaaaaata tatgggagca tacgaaaata tataaactat atgtaaaaaa aatacgttta
     3961 aatatgaatt atatagctct gaacataaaa aaagttaaag tcctaccgta aaagagtgta
     4021 ttttgaataa gataacaaat atgataaaat tggtcttgtt gaaattttta ggggggatat
     4081 gatgacaaag aaactagtaa tatcaaaaac aaagttgttt atttttataa tagcatttat
     4141 ttctatattt atatatttat ttggaagtaa aaacacactt ataggtgtag gtatagtaac
     4201 agctatgcta acactcctgg aaagagattt aactatttct ccaattaaaa atttactaaa
     4261 gtatttagca ataaatataa tcttaggaat actatcattt tttgcagtac aaaatatgta
     4321 tttaggcgtt ttattaaatt ttatagcatt atttataata gggtatgtat ttagttatga
     4381 cttaaaaaga gcagtatatg taccttttgg tctaatgtat atatttatga taagtatacc
     4441 tgtaggaaaa agtgaatttc ctatgagatt gttggcttta gctgctggag cagttataat
     4501 aatgattgct caatttgtca tgaatagaaa taggatgaaa aatgtcggag ataaagaact
     4561 gatatctata tgtgatgaat tattagaaaa aattagtctt ttaaaaaata ctattaatga
     4621 tgatagttct ataattaaat ctagtatgag aaaagtagat agctgtaatt atagaataaa
     4681 ttctatatct aaaaatttga aaatggttat ttttgataat agaaaagatg acttttatat
     4741 tagtattaga ggcatagaca taatgaatat tttattttca ttagaaagaa taaatttaat
     4801 cctggaaaga tacaaaaagg atagtaaaga atttgaagat gaagatataa aaaatatttt
     4861 agaagaaagt agcataaata gtaaatcaga tgttctagtt gtagcaacaa aagaagtaaa
     4921 ctatattaag ttgtgtttag aaaataaaga taccataact gataaagaga tattaggatt
     4981 tagagattat gttattgctc aggatactaa aaatataaat ttaaaagaaa tttatagtgt
     5041 tttagaaaat ttatatgagt ttttagtaga ttataaaaaa gtaaaatcaa gagacgaaaa
     5101 gaaagctgaa agaaaatcaa agatacctcg tgaatttaaa cgactatcaa tatataagaa
     5161 aaactttaat ttaaattcta taagattttc ttatgcagta aagattgctt tagctacagc
     5221 tgtagcgggt tttattatgg attatttcca tttaaaggat ggaagatgga taatgttaac
     5281 tgtcttttca ttaacacaac cttatgcaga aaattgtata caaaggtcta gaaaaagaat
     5341 agaaggtaca tttataggag ctgtaatatt tattgtatta ttttcaataa taaaagatag
     5401 tacattaagg tcgttaatag ttcttttagc agggtatata aactcttatg tagttgatta
     5461 tagaaaatta atggtatgtg taactgtttc agctcttggt tcagcagtag ttatgggaga
     5521 cccaaatgtt ttaacaataa gcaggatttc atatgtagca ttaggagcaa ttattgcatt
     5581 aatcgtaaat aagtttatat taccatatga tgctaaaaca ggatatcaac atgttataga
     5641 aatgtataaa ggaatagtta aaaatataat tgatgaagtc aataagtcta tagaaaatgt
     5701 agctgatgtt tactatataa aaaatttatt attgatacca tcacttattg aagatagact
     5761 tatgttgata aatgctatat ataaagataa acatcaagag gattttttag aaaatcagaa
     5821 attactaata agtaacatgt ataatctata tataaatgtt aataaaaata aaataaaaga
     5881 tgaagatgtt gaaaaaatat tgagagatac taattatatt tcaaattaca atgtagacaa
     5941 gtatgatgaa ggaagaaatg tgattttaga aagtattgta aatactaaaa gtctaggtga
     6001 caagataata tgtctaaact tactgcaaac tctaaatggt gtcaaagaaa tgtatagaat
     6061 aagtaatata actaaagttt caatagaaga agcagc
//
LOCUS       DAEPXF010000209         5932 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain HB27
            SAMN13376389-rid12479423.denovo.217, whole genome shotgun sequence.
ACCESSION   DAEPXF010000209 DAEPXF010000000
VERSION     DAEPXF010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13376389
            Sequence Read Archive: SRR10518432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5932)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5932)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001069624.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001069624.1
            Genome Coverage       :: 315x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/15/2021 01:54:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,165
            CDSs (total)                      :: 4,076
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 89
            rRNAs                             :: 10, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 10 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 19 of 69
            Pseudo Genes (incomplete)         :: 44 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 10 of 69
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5932
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13376389-rid12479423.denovo.217"
                     /strain="HB27"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="China: Hebei"
                     /collection_date="2017"
     gene            206..2218
                     /locus_tag="KRL67_003408"
     CDS             206..2218
                     /locus_tag="KRL67_003408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBH1524249.1"
                     /translation="MKKSVALVNDSRKDLIDFLENNLKLVFGDSININRYFINEINDN
                     DIINDDVILVMSVERLDKIINNILDKKKVIVVRRTFREDKIYNLLSLPQGTNALIVND
                     SDETTLETISLFYKIGVTNIRPIPYMNDNNYKNIKIAITPGVPEKVPSFISDIFDLGH
                     RYIDISTFIEIINLLQIDSKEIQSNLVKYSEEIISLDTGIKDKYKELFLKIEELDTIL
                     NLSKDGILFTSKDGEINTYNNKVKDILDISEDIYGKYIEDIFVDSLKVLLNEKEILDK
                     VVIFNRKYINVNKKNIYNRDEKMGTYYSLQEITYIKKLEQNLTKKLREKGQIAKYTFK
                     DIKTTSPKMLECIDLAKKISKSDLSILIRGESGTGKELIAQSIHNNSNRKNQPFIAVN
                     CAAVPENLLESQLFGYDKGTFTGGLKDGKQGLFELANNGTIFLDEIGDMPLELQTKLL
                     RVLQEKQIMPVGSHNVINIDVRIISATNKNLEQMIDNSQFREDLYYRLNTIPINIPPL
                     RERKEDILIIMEDLINKKLVITPEAKKLIQNYKWKGNIRELQNVTSYLNIMCEDIVLE
                     KDLPPNLRSSVNKNPSLKLKYNKNDILNILEILVLNKESDVGIGRGLILKTLLDKNLQ
                     ITEGKIKKIFEYLKKEELIICGSGRYGSKITQKGEDFYNKLKYKGL"
     gene            2587..3807
                     /locus_tag="KRL67_003409"
     CDS             2587..3807
                     /locus_tag="KRL67_003409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HBH1524250.1"
                     /translation="MQQDLKLKYQTKELEKDFVIESCNSIKPWLINVRRELHKIPELA
                     LEENLTKQKVISYLKEIGIDYMEFTKHNGIMAYILKENADKTICIRADMDALPIEEEN
                     DIPYKSIYSGKMHACGHDAHTTMLLGACKVLHSIKDKLNVNVKLLFQPAEEGFGGAKF
                     LVEDGCLENPKVDYIFGLHVMPHIETGFIETKYDTLNASVDTIKICVKGKRAHGAYPE
                     NGIDAIVTASQIVTSLQTIISRNLEPNNAAVLTIGKIYGGDAHNVICEDVKLEGTLRT
                     LNSKTRNFMIDKISKIVEDTASAFGCVGTLHVSDENYPAVINEKELVDTVISSTKELL
                     GEEKFILRANPSLGGEDFSFYTEHCKGAFFHLGCKNDEKGLISPLHTSSFNIDEDCLP
                     IGVMMHVMNTLYFN"
     gene            3844..5193
                     /locus_tag="KRL67_003410"
     CDS             3844..5193
                     /locus_tag="KRL67_003410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBH1524251.1"
                     /translation="MEKAKKLGLFSMILLGINSIIGSGIFLLPGKVYNLAGQNSMFIY
                     IFATLLVLSILLCFAEVGSMFDKNGGAYLYSKKAFGDFIGFEVGTMSWVIRIISWSTL
                     AVGFATALGSFWPESATEYKGYIAATLVTLLSINSLFGIKSTKIMNNVITIAKLVPLI
                     VFVIVGIFFIKFANIVPSGNVVNSSMGPAIILVFYAFTGFESFIVASGEMENPKKNLP
                     IALITTIFICAIIYILIQVVCMGILGDRLFENSIPIADASSVFLGNYGKVFISVATLI
                     SIFGINIGSSIVTPKCGSSLAEEGSLPAFIGKTNKYGAPYVAIIISLICCIPLVLTGS
                     FEQLAVMSVIARFAQYIPTCLSVIVLRKRTDVKASFKIPFGPVIPIVAILGSLWLLQQ
                     AWAEDISKPITQNRVLIGLGAMLLIAPLYIFMKKNKEIEHKNKSEDSKLYISREKIQ"
     gene            complement(5276..5743)
                     /locus_tag="KRL67_003411"
     CDS             complement(5276..5743)
                     /locus_tag="KRL67_003411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HBH1524252.1"
                     /translation="MKFLNVLIVVEDIEKSKKFYYDVLGLKVICDFGENVVLEGNISL
                     QEKKLWLEFINKSDSEVKFNGNDAELYFEEDYFDTFVERLSTMRDIDYVHLAIEHRWG
                     QRAIRFYDLDGHIIEVGETMSSVCRRFLDSGLSIDEVAKRMDVTVEYIESVLE"
BASE COUNT         2390 a          730 c          866 g         1946 t
ORIGIN      
        1 atatacatac agtttaagtt ttaagcaaca attaggggga tttggtgaca agatttttaa
       61 ttataagaaa gaaagtgtct taatgcaact tttagaaagt cattatagac actttctttt
      121 ttatttgata aatttaaatt atcaaaatta gattgcttta tacaactatt ttgttggaaa
      181 ctatttatta tataaggaga aataaatgaa aaaatcagta gctctggtaa atgatagtag
      241 aaaagattta atagactttt tagaaaataa tttgaaatta gtttttggag attctataaa
      301 tattaataga tacttcataa atgaaataaa tgataatgat attataaatg atgatgtgat
      361 attagtaatg tcagtagaaa gattagacaa aattataaat aatatactag ataagaaaaa
      421 agtgattgtg gtaagaagaa cttttaggga agataaaatt tataatctac tatctttacc
      481 acagggtaca aatgctctaa tcgtaaacga ttctgatgaa actactctag agactataag
      541 tttattttat aaaattggag tgactaatat tagaccaatt ccatatatga atgataataa
      601 ctataaaaat ataaaaatag ctataactcc aggagttcca gaaaaagtac ctagctttat
      661 atcagatata tttgatttag gtcacagata tatagatatc tctacattta tagaaataat
      721 aaatttatta caaattgatt caaaggaaat acaatctaat ctagttaaat actcagaaga
      781 aataataagt ttagatactg gtataaaaga taaatataag gaattatttt taaaaataga
      841 agaattagat acaatattaa atttatcaaa agatggcata ttatttactt caaaagatgg
      901 tgaaataaac acatacaata ataaggtaaa agacatttta gacataagtg aggatatata
      961 tggaaaatat attgaggata tatttgtaga tagcttaaaa gttttattga atgaaaaaga
     1021 gatactagac aaagttgtca tattcaatag gaaatatata aatgtaaata aaaaaaacat
     1081 atacaataga gatgaaaaaa tgggaacata ttatagttta caagaaataa catacataaa
     1141 aaaattagaa caaaatctta ctaaaaagtt aagagagaaa ggacaaatag caaaatatac
     1201 atttaaagat ataaaaacaa ctagccccaa aatgcttgag tgtatagatt tagctaagaa
     1261 gatttctaaa tctgacttaa gcatacttat cagaggcgaa agtggaactg gcaaggaact
     1321 catagctcag tctatacata acaactcaaa tagaaaaaat caacccttta tagcagtaaa
     1381 ttgtgcagct gttcctgaaa atttattaga aagtcagcta ttcggatatg acaaaggaac
     1441 ttttactgga ggtctaaaag atggaaaaca aggattattt gaacttgcta ataatggaac
     1501 aatattctta gatgaaatag gcgatatgcc tttagaatta caaacaaaac tacttagagt
     1561 tcttcaagag aaacagataa tgcctgttgg ttctcacaat gtgattaata tagatgttag
     1621 aatcatatct gctacaaata agaatttaga acaaatgatt gataatagcc aatttagaga
     1681 ggatttatat tatcgattaa atacaattcc aataaatata cctcctctaa gagaaagaaa
     1741 agaagacata ttaattatta tggaagattt aattaataag aaattagtaa tcacaccaga
     1801 agcaaaaaaa cttatacaaa actataagtg gaaaggtaat ataagggaac ttcaaaatgt
     1861 tacatcctat ttaaacatta tgtgtgaaga tattgtatta gagaaagatt tacctccaaa
     1921 cttaaggtct tcagttaaca aaaatccatc attaaaatta aaatataata agaatgatat
     1981 attgaatata ttagagattc tagttttaaa taaggaatca gatgttggta ttggtagagg
     2041 attaatttta aaaacactac tcgataagaa ccttcaaatt acagaaggaa aaattaaaaa
     2101 aatatttgaa tatctaaaaa aagaagaact tataatttgt ggttcaggaa gatatggaag
     2161 taaaataact caaaaaggag aagattttta caacaaatta aaatataaag gtctataaat
     2221 ttataatcta ttaaacaaag aaatctattc aggagctaaa tactccttca ataggttttt
     2281 ttatttttca agtaccccaa aattataact tattttgtac ataaataata ggaattaata
     2341 ggaataggat atttttttat ttcctatttc ttttatataa gctaagcata taagataaat
     2401 tttctccaaa ataatatttt ttactgaaaa ttgcaactat aaaatcgttt tttacattat
     2461 atatatggat aaatgattct gtttattcaa tattcttata aaaagtaaaa atcttcatct
     2521 aaaattatgg cataataatt gcttattata ttaaccaaac aataaaaagc aattggagga
     2581 ataaaaatgc aacaggattt gaaattaaaa tatcaaacca aagaattaga aaaagatttt
     2641 gtaatagaaa gctgtaattc tataaaacct tggcttataa atgtaagaag ggaattacat
     2701 aaaattccag aattagccct tgaagaaaat cttactaaac agaaggtcat ttcttattta
     2761 aaagaaatag gaattgatta tatggaattt acaaaacaca atgggattat ggcatatatt
     2821 ttaaaagaaa atgcagataa aactatttgt ataagagcag atatggatgc ccttcctatt
     2881 gaagaagaaa acgatatacc ttacaaatca atatattctg gaaaaatgca cgcttgtggc
     2941 catgatgcac atacaaccat gttacttgga gcatgtaaag ttttacattc aataaaagat
     3001 aaacttaatg taaatgtaaa gttattattt caaccagcgg aagaaggttt tggaggagca
     3061 aaattcttag ttgaagatgg atgtttagaa aatcctaagg tcgattatat attcggactt
     3121 catgttatgc cacatatcga aacaggattt atcgaaacaa aatatgatac attaaatgct
     3181 agtgtcgata ctataaaaat ttgtgtcaaa ggtaaaagag ctcatggtgc atatcctgaa
     3241 aatggtattg atgcaatagt tacagcttca caaattgtta catcattaca aactataata
     3301 agtagaaact tagaaccaaa taatgctgct gttttgacta taggtaaaat atatggtggt
     3361 gatgcacaca atgtaatatg tgaagatgta aaattagaag gcaccttaag aaccttaaat
     3421 agcaaaacaa gaaactttat gattgataag atatctaaaa ttgttgagga tactgcatct
     3481 gcatttggct gtgttggaac tttgcatgta agtgatgaaa attatcctgc tgttataaat
     3541 gaaaaagaat tagtagacac agttatatca agtactaaag aattacttgg agaggaaaaa
     3601 tttatattga gagcaaaccc ttctttgggt ggtgaagatt tttcttttta cacagaacat
     3661 tgcaaagggg cgttcttcca tttaggatgt aaaaatgatg aaaagggatt aatttcacct
     3721 cttcatacct ctagctttaa tatagatgaa gattgtttac caataggtgt tatgatgcat
     3781 gtaatgaata ctttgtattt taattagtac agtaattaat aaaagaatag gagataaatt
     3841 aatatggaaa aagctaagaa attaggtcta tttagtatga tattattagg gattaatagc
     3901 ataataggtt caggtatatt cttactacca ggtaaagttt ataatctagc aggtcaaaac
     3961 agtatgttta tatacatttt tgctacactc ttagttctat caatactttt atgttttgca
     4021 gaagttggaa gcatgtttga taagaatgga ggagcatatc tatactccaa aaaagctttt
     4081 ggagatttta taggatttga agttggtaca atgtcttggg ttattagaat tatatcatgg
     4141 tctactttag cagtaggatt tgcaacagct ttaggttcat tttggccaga atctgccact
     4201 gaatacaagg gctatatagc agcaacactc gtaacactac tttcaataaa tagcttattt
     4261 ggaattaaaa gcacaaagat aatgaataat gtaataacta tagctaaatt agtaccatta
     4321 atagtttttg taatagttgg aatattcttt ataaaatttg caaatattgt tccttcagga
     4381 aatgtagtta actctagtat gggtccagcc attatattag tattttacgc atttaccggc
     4441 tttgaatcat ttattgtggc aagtggagaa atggaaaatc ctaaaaaaaa tcttccaatt
     4501 gcattaataa ctacaatatt tatatgtgca ataatttata tattaattca agttgtatgt
     4561 atgggtatac ttggagatag actatttgaa aacagtattc caattgcaga tgcttcaagt
     4621 gtatttttag gcaattatgg taaggttttc atatctgtag caacattaat atctatattt
     4681 ggtataaata taggttcatc aatagtaact cctaaatgtg gttcttctct agcagaagaa
     4741 ggttctcttc ctgcatttat aggaaaaaca aataaatatg gtgctccata tgtagcaata
     4801 ataatatctt taatttgttg tataccactt gtgctaactg gaagttttga acaacttgca
     4861 gtaatgagcg taattgcaag atttgctcag tacataccaa cttgtttatc agtaatagtt
     4921 ttaagaaaaa gaacagatgt taaggcctct tttaagattc catttggtcc tgttatacca
     4981 atagtagcta ttttaggtag cttatggttg ttacaacaag cttgggcaga agatatttca
     5041 aaacctataa ctcaaaatag agttttaata ggattagggg caatgttgtt aatagctcct
     5101 ctatatatat ttatgaaaaa aaataaggaa atcgagcata aaaataaatc agaagattct
     5161 aaattatata tttcgagaga aaaaattcaa taatatttta caaactattt attaaaaata
     5221 ataaataaag gcaaaagcac agattagctg acgcctttat tttaatataa tccatctatt
     5281 ctaaaactga ttcaatatat tcgacagtta catccattct tttagctacc tcatctattg
     5341 aaagaccact atctaaaaaa cgtctacaca cagatgacat agtttctcca acctcaataa
     5401 tatgcccatc taaatcataa aatctaattg ctctttgacc ccacctatgc tctatagcta
     5461 gatgaacata atctatatct ctcattgtac tcaatctttc aacaaaagta tcaaaataat
     5521 cttcctcaaa atacagctca gcatcatttc cattgaactt aacttcactg tcactcttat
     5581 taataaactc taaccaaagc tttttttctt gtaatgatat attcccttct aatacaacat
     5641 tttctccaaa atcacagatt acctttaacc ccaaaacatc ataataaaac ttttttgatt
     5701 tctcaatatc ttcaactact attaaaacat ttaaaaactt catattacct ccttaatata
     5761 cacatacttt atttatatta tataattaga attaaactat aaaggtttca ataaggtcta
     5821 gtataaagaa aaaaattata agtcttattg aaatagttaa gttaattgta tcatataata
     5881 taaattttaa tcttgtaaaa aacagacaca ttcatacttt ttaggtttaa at
//
LOCUS       DAEPXF010000210         5923 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain HB27
            SAMN13376389-rid12479423.denovo.218, whole genome shotgun sequence.
ACCESSION   DAEPXF010000210 DAEPXF010000000
VERSION     DAEPXF010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13376389
            Sequence Read Archive: SRR10518432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5923)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5923)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001069624.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001069624.1
            Genome Coverage       :: 315x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/15/2021 01:54:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,165
            CDSs (total)                      :: 4,076
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 89
            rRNAs                             :: 10, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 10 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 19 of 69
            Pseudo Genes (incomplete)         :: 44 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 10 of 69
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5923
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13376389-rid12479423.denovo.218"
                     /strain="HB27"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="China: Hebei"
                     /collection_date="2017"
     gene            571..975
                     /locus_tag="KRL67_003412"
     CDS             571..975
                     /locus_tag="KRL67_003412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HBH1524253.1"
                     /translation="MIKITIPGRPISKSNFKLHNINGQAWMPSKGKYSKYLAYENMIA
                     GFINQQYQGETVEENLITVLKLFFPNKRMGDLHNYPKSICDGIEKSGIIKNDKQLKPV
                     LLFDFIDKDNPRVEIELYPVSKYDISYNIFEK"
     gene            complement(1231..2226)
                     /locus_tag="KRL67_003413"
     CDS             complement(1231..2226)
                     /locus_tag="KRL67_003413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HBH1524254.1"
                     /translation="MRNLGNTNMKIKRVGFGGIPIQRITQDDTNLVINELEKQGINFI
                     DSARGYTISEEAIGIAIEGKRDKFFLATKSMSRDYDSMKRDVEISLNNFKTDFIDLYQ
                     FHNVKEEEYDNLFKDKMAYSALLEAKEQGKIKHIGITSHNLNTIEKAIEDGKFDTIQF
                     PYNIVEGQADEVFKKAHEKGIGIIVMKPLAGGALDNATLAIKYILSKDYIDVVIPGME
                     SVEQVRQNVAVLENLVLDEKDNKEIEEIRNSLGKKFCRRCEYCMPCAVGINIPLSFLC
                     EGYYTRYGLKEWAKEKYEVMDVKPTECIDCGLCESRCPYELPIREMLKTVVEKLG"
     gene            complement(2510..3346)
                     /locus_tag="KRL67_003414"
     CDS             complement(2510..3346)
                     /locus_tag="KRL67_003414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HBH1524255.1"
                     /translation="MIKLGTHLSIAKGFANAAETAVYIGANTFQFFSRNPRGGNAKEF
                     DEKDIIKFQEIRKANNFAPLLAHAPYTMNLGGTKDDVYEFATRVIDEDIRRMDSLGIE
                     YMCFHPGSHVGGGVDFGIDRIVKGLNNAIKGDENITILLETMSGKGTEIGFKFEQLKS
                     IIDKIEHKEKIGVCLDTCHIFSAGYDIVNDLDGVLEEFDKVIGIERLKTVHLNDSMMP
                     FGDKKDRHAPIGEGKIGLKALIDFMEHPSLKHLPFFLETPFDDEGHKRELKMIKEILS
                     SK"
     gene            complement(3834..4397)
                     /locus_tag="KRL67_003415"
     CDS             complement(3834..4397)
                     /locus_tag="KRL67_003415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBH1524256.1"
                     /translation="MKEVLVLRDLECIKAIAHPRRIDILKAFDKSPLSAKQLSQLLEE
                     PHAKINYHIKMLYKVGILELVEEKIKSGIVEKYYYPSAKNVVIGNRILNFSLDNGEEK
                     EELYISKFENMSEVFYKAAEEDVLENENIVDYHDISLTHDELVELSDTMKSKIDEILN
                     KRQHNVEGSKYDIAMVIVPTLEEECPS"
     gene            complement(4495..5424)
                     /gene="glsA"
                     /locus_tag="KRL67_003416"
     CDS             complement(4495..5424)
                     /gene="glsA"
                     /locus_tag="KRL67_003416"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HBH1524257.1"
                     /translation="MNTLDETLLKEIISSNKKYTNYGQVASYIPELKNARRNDLGICI
                     IDSENNLYSAGNCSTKFTIQSISKPIVLAMALMDNDWEDVFSNVGMEPSGDPFNSIMK
                     LEINDTKKPCNPMINAGAIVTTSLINGSCLEEKEERMLSFFRKLAKNDNIGINYDVYK
                     SEKMTGDRNRAMAYLLKSDGFIRGNVEDVLDLYFKQCSIEIDSVDLARIGINLANYGV
                     DIENGEHLMSEMVSRIVKTFMMTCGMYDASGEFAIKVGIPAKSGVGGGIMASVPGRMG
                     IGVYGPALDKKGNSVAGVKVLEELSNKLKLNIF"
BASE COUNT         1970 a         1083 c          565 g         2305 t
ORIGIN      
        1 ttaatttttt tatctactaa ctcttttaga tacttagcct ttttacctat ttaatttttt
       61 acctactaac tcttttagat acttaatctt ttttagatat ttaatttttt tatctactaa
      121 ctcttttaga tacttagctt ttttaatcta ttcaaccttt tttacccact taaccttctt
      181 accttttaat ataaactcta aagcttcaaa tatcattagt tatattttga cattcttcct
      241 atagttaatg caagaagatt gattttttct tatatttaat attccattta cattgccatt
      301 tattaagtct ttacttgact tatatgggtt tctttttact ctctttacta aacttatact
      361 tattaatatt atgaaggtct tataatagtc taatatgaat atgtccagca tatctcaaaa
      421 tttaaaactt gtaagcaaaa cacttatctt atattactac atctaaagtg gaagatttta
      481 caaagaaact ctgtaaaaca ctttaaataa caatttgctt ttaaatcata tacagattat
      541 aataaatact gtataaggat ggtgaaaaat ttgattaaaa taacaattcc aggtagacct
      601 attagtaaat ctaattttaa gcttcataac ataaatggtc aagcatggat gccttcaaag
      661 ggaaaatatt ctaagtatct tgcatacgaa aacatgattg caggttttat aaaccaacag
      721 tatcaaggag aaactgttga agaaaatcta ataacagtct tgaaattatt cttcccaaat
      781 aagagaatgg gggatttaca taattatcca aaaagtattt gtgatggtat tgaaaaaagt
      841 ggaataataa aaaatgataa acaacttaag ccagtacttt tatttgattt tatagataag
      901 gacaacccta gggttgaaat tgaactttat ccagtatcta aatacgatat atcctataat
      961 atttttgaaa aataaagatt ttatgtgtta ttcgacatta tattgatgct ccagtatttg
     1021 aaactatact aatacaacaa taataattgt gttatatcta tactcaaaca aatattttta
     1081 aaaacattaa gcaaatattt agtaagtatt aaacaaatat taaatttata taatagctat
     1141 aactatgtaa atttaatatt tgtatataag accacatcta ataaaaagaa atcggagtaa
     1201 atttaataca ccgatttcaa taaataaaat ctatcctagc ttttcaacaa cagtcttaag
     1261 catttctctt ataggaagtt catatggaca tctactctcg caaagcccac agtcaataca
     1321 ctcagtaggt ttaacatcca taacctcata tttttcctta gcccactctt ttaatccata
     1381 tctagtataa taaccttcac ataaaaaact taatggtata tttataccta cagcacaagg
     1441 catacaatat tcacatcttc tacagaattt tttacctaaa gaatttctta tttcttcaat
     1501 ttccttatta tctttttcat ctagcacaag attttcaagt acagcgacat tttgtctaac
     1561 ctgttcaaca ctctccatac ctggtataac tacatctatg taatctttag atagtatata
     1621 tttaatagcc agtgttgcat tgtctaaagc acctccagct aaaggtttca ttactattat
     1681 cccaattcct ttttcatgag cttttttaaa cacttcatct gcttgtcctt caactatatt
     1741 ataagggaat tgtatagtgt caaattttcc atcttctatg gctttttcta tagtatttaa
     1801 attgtgacta gttataccaa tatgttttat cttaccttgc tcctttgcct ctaaaagggc
     1861 actataagcc attttatctt taaaaagatt atcatattcc tcttctttaa cattatggaa
     1921 ttgatataaa tctataaaat cagttttaaa attatttaaa cttatttcaa catctctctt
     1981 cattgaatca taatctctgg acatactttt agtagctaag aaaaatttat ctctttttcc
     2041 ttcaatggca attccgattg cttcctcact tattgtatac cctcttgcac tatcaataaa
     2101 gtttattcct tgtttttcta actcatttat aactaagtta gtatcatctt gagtaatcct
     2161 ttgtattgga atgcccccaa agccaactct ctttattttc atgttagtat tacctaaatt
     2221 tctcatatct ttccccacct aacttttaat aattttttat ataatataga tatgtcacct
     2281 ttaattatac aacaaattat gagaatcttt gcaatatatt tacatactat tcataatata
     2341 attatatttt atatcagtct attcataata taatcatact ttatacctag ttaatttgta
     2401 tacaattttt gttattccta tccattaatt taatttatca catttcatga ttgtatctcc
     2461 accctaaata tgttgagggc ggagtatttc acatattttt aataaagttt tatttactag
     2521 atagtatttc ttttatcatc ttaagctctc tcttgtgacc ttcatcatca aaaggagttt
     2581 ctaagaaaaa tggtaaatgt tttagactag gatgttccat aaaatcaatt aatgctttta
     2641 atcctatctt tccttcccct ataggagcat gtctgtcttt tttatctcca aatggcatca
     2701 tactatcatt tagatgaaca gttttcaatc tttctatacc tataacctta tcaaattcct
     2761 ctaagacccc atctaaatca ttcactatat cataaccagc tgaaaaaata tgacatgtat
     2821 caagacacac acctattttt tctttgtgct caattttatc tattatactt tttaattgct
     2881 caaatttaaa acctatttca gttccctttc ccgacatggt ttcaagaagt atagttatat
     2941 tttcatcgcc ctttatggca ttgttcaatc cttttactat tctatctata ccaaaatcta
     3001 caccaccacc tacatggctt ccaggatgaa agcacatgta ttcaattcct aatgagtcca
     3061 tccttcttat atcctcatct attacccttg ttgcaaattc gtatacatca tcttttgtgc
     3121 cacccaaatt catagtatat ggtgcatgtg caagcagtgg tgcaaaatta tttgcttttc
     3181 taatttcttg aaatttaatt atatcttttt catcaaattc tttagcattt cctcctcttg
     3241 gatttctact aaaaaattga aatgtatttg caccaatata tacagctgtc tcagccgcat
     3301 tagcaaatcc ttttgctatt gaaagatgtg ttcctaattt aatcatatta atctcctttt
     3361 atttgtacta caacttgtac tcaattttaa ttttgaacta ctccacctta actacattca
     3421 gccaacatat gaagatggag attcctattt gatagtactt tttcactaaa aattcataag
     3481 ctctaaggaa tgctctatcc caactaacct cattcctctt ttaacatatt tctagtattg
     3541 atttaattta tattaatttc taattttaat atttacactt taattatacc cattttaatc
     3601 aatttcaaat attataatct tacatacttt gtatattatc taagcacttc ttatatagtg
     3661 tatacatctt gcacactata tattatttac aaatacaata ggtataagaa aacaaaaaaa
     3721 tagagattaa aaatcattgt tttacccata ccaaaaacta ttttttgatt attaagtaaa
     3781 ttttgatgtt caatctctaa tattatatct ttaattaatc cataattaat tctttaacta
     3841 ggacattcct cctcaagagt aggaactata accatagcta tatcatactt agaaccttca
     3901 acattgtgtt gtcttttatt taaaatttca tctatctttg acttcatagt gtcactaagt
     3961 tcaactaatt catcatgagt aagagaaata tcatgataat caactatatt ttcattttct
     4021 aaaacatctt cctctgctgc cttataaaaa acttcactca tattttcaaa cttcgatatg
     4081 tacaattctt ctttttcttc cccattgtct aaagaaaaat taagtatcct attccctata
     4141 accacatttt ttgcacttgg ataatagtac ttttccacaa tccccgactt tattttttcc
     4201 tcaactaatt ctaaaatacc aactttgtat aacattttta tatggtagtt tatttttgca
     4261 tgaggttctt ccaataattg agataattgt tttgctgaca atggcgattt gtcaaatgct
     4321 tttagaatat ctattctacg tggatgcgca atagccttta tgcactccaa atctcgtaat
     4381 accaatacct ctttcattga cccctcctaa aaaatttatt taattcatat ttctttacaa
     4441 tttaattata tattaaatta tatgttgttg tcaaaaaaat cctttcttaa tattttaaaa
     4501 aatatttaat ttgagtttat ttgacaactc ctctaaaact ttaactcctg cgacgctgtt
     4561 accttttttg tcaagcgcag gtccataaac accaatcccc atgcgtcccg gcacagaagc
     4621 cattatacca cccccaactc cagattttgc aggaatgcca actttaatag caaattcccc
     4681 agaagcatca tacattccac aagtcatcat aaatgtcttg acaatcctag aaaccatttc
     4741 actcattaaa tgctcgccat tttctatatc tactccataa tttgcaagat ttattccaat
     4801 tctcgctaaa tctacagaat ctatctcaat cgagcattgt ttaaaataaa ggtctagaac
     4861 atcttctaca ttccctctga taaatccatc acttttcaat aagtaagcca tagctctgtt
     4921 tctatctcca gtcatttttt cagacttata gacatcatag tttattccta tattatcatt
     4981 ttttgctaac tttctaaaga aactaagcat tctttcttct ttttcttcta aacaactccc
     5041 atttataagg gaagtagtta ctatagcccc tgcatttatc attggattac agggtttttt
     5101 tgtgtcatta atttcaagtt tcattattga attaaatggg tccccacttg gctccattcc
     5161 aacgttggaa aaaacgtctt cccaatcatt atccataagt gccatagcaa gcacaattgg
     5221 ttttgaaata ctttgtatag taaactttgt gctacagttt ccagcactat aaagattatt
     5281 ttcgctatct atgatacaaa ttcctaaatc atttcttcta gcgtttttta attctggtat
     5341 gtaactagcg acttgcccat aattagtata cttcttatta gaacttataa tttcttttaa
     5401 taatgtctca tctaacgtat tcatatgcac ctcttatatt atgttatata ttctatttta
     5461 gcacaagttt atactataat aaaacaatgt taaaaaaaat tatactttaa aattattttc
     5521 tacaagaatt aattcatttt tcaaacaatt taaatagttt aaatagctta ataatttagt
     5581 aattcaataa tttaacaacc tagcaattta gcaatataat aatctagtaa tctatcaatc
     5641 taataatcta gtaatttaac aacctagtaa tctatcaatc taataatcca gtaacttaac
     5701 aaccttgtaa tctatcaatc taataatcca gtaacttaac aaccttgtaa tttagcaatc
     5761 tagtaattca ataatttagc aatctaataa tccagtaatt taacaacctg gtaattcagc
     5821 aatctaataa cctagtaatt tagcaatcta gtaatctagt aatctaaatt tttataacat
     5881 caaaacttct aaccaaacat cttttactct tttactcttt tac
//
LOCUS       DAEPXF010000211         5907 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain HB27
            SAMN13376389-rid12479423.denovo.219, whole genome shotgun sequence.
ACCESSION   DAEPXF010000211 DAEPXF010000000
VERSION     DAEPXF010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13376389
            Sequence Read Archive: SRR10518432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5907)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5907)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001069624.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001069624.1
            Genome Coverage       :: 315x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/15/2021 01:54:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,165
            CDSs (total)                      :: 4,076
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 89
            rRNAs                             :: 10, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 10 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 19 of 69
            Pseudo Genes (incomplete)         :: 44 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 10 of 69
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5907
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13376389-rid12479423.denovo.219"
                     /strain="HB27"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="China: Hebei"
                     /collection_date="2017"
     gene            105..725
                     /locus_tag="KRL67_003417"
     CDS             105..725
                     /locus_tag="KRL67_003417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HBH1524258.1"
                     /translation="MQYIQELLDLTQNYWVLATIIGLLSAFIESFIPALPLVAIVTAN
                     AAIQGLFIGCLLSWIGSGLGTTSLFLLISRFNDSKLFNKLRNSKTEKAISWMDKQGFK
                     LLFIAYACPFMPGCLVTIASAFGKKDVKDFVPAMLAGKFVMFIVISYVASDIEGFITS
                     PLKIVSFILLVFLSWKIGNRVNKNLENHNYDFHHKKHHNDKDDKMI"
     gene            complement(890..1426)
                     /locus_tag="KRL67_003418"
     CDS             complement(890..1426)
                     /locus_tag="KRL67_003418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HBH1524259.1"
                     /translation="MIKETHERGGYIFALLILPFINNLYLVKYDIAYKVVLIIVYIYF
                     AYLGSLFPDIDMRGSYISKKFMLIYKLFGSRFRHRGFTHSLIALLLIYSFFKSLTIFT
                     NNNIVFSCLSSGFIIGYLSHLCLDLITKEGIELFYPITINISLLPIKTNSKTEKFISK
                     LLNFIVIFLIGYQFYILL"
     gene            complement(1539..2213)
                     /locus_tag="KRL67_003419"
     CDS             complement(1539..2213)
                     /locus_tag="KRL67_003419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000094049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HBH1524260.1"
                     /translation="MSLLIISQCITSFAFTPENNKFKVKPLPYAYDALEPYIDKETMK
                     LHHDKHYQAYVDKLNAALEKYPELYNYSLCELLQNSDSLPKDIATTVRNNAGGAYNHK
                     FFFDIMTPEKTIPSESLKEAIDRDFGSFEKFKQEFQKSALDVFGSGWAWLVATKDGKL
                     SIMTTPNQDSPVSKNLTPIIGLDVWEHAYYLKYQNRRNEYIDNWFNVVNWNGALENYK
                     NLKSQD"
     gene            2416..4290
                     /gene="asnB"
                     /locus_tag="KRL67_003420"
     CDS             2416..4290
                     /gene="asnB"
                     /locus_tag="KRL67_003420"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HBH1524261.1"
                     /translation="MDGNISIYYKGDISKKEDIKFKIKESVNNTRLSSRIEYKDEEKT
                     FDFERFNVIDAIYGNKPFEKDGKVIVFNGNIYNYHEIKEELIKKGHNFTTNDDIEVLL
                     ASYIEFGKNCVHKIKGMFNFIIYDRENESIFGARDLFGIKPLYYINKENAIIFSSEYK
                     FLLEYMKNLNINERSLQSYFSFQYVLPEDTMIQGIRLIPAGHYFRVENGILSLKRYNK
                     LEFRSSTKFFYTKNHLGNRDVNEDDIRNVVVDSITNHMEEDKEIGTFLSGGIDSSIIA
                     TVASQINPNIKSFSAGFSVKGYSELEVAKKTADKLGIENIQINITQDEYIKSLPNVIY
                     SLDDPIADPSEVGLYFLIKEAGKHVKVVLSGEGADELFGGYNIYKEYSTMKSVVNSPT
                     YIKSMLGRVSELMPNIKGRNYLYRATTPLEKRYIGNAKVFENSEVKRFFFKYKEKNIY
                     EYLLSNLYRDAQKNNYDYISKMQHIDVNTWLQGDILQKISKLSTAEQVELRVPFLYKD
                     VFDVAKNLRMEQKINKNNTKVLLRDAFREIVPEHVVQRKKLGFPTPIRVWLKDSLGDI
                     VKETISNSNVDDFIDKKYVIKLLDTHLKGYRDNSRKIWTIFTFCLWHQLFIEHKNVEY
                     "
     gene            complement(4382..5818)
                     /locus_tag="KRL67_003421"
     CDS             complement(4382..5818)
                     /locus_tag="KRL67_003421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HBH1524262.1"
                     /translation="MICTIKPLRISIKKSNEPLIEFISDTTYKNKSSSYIKEVYFTRY
                     IGVDDSIDKYIEKIKNIDTYFFNNTTIPYIRLSNLKINFDKEQTDKMLKIFNIYDSSK
                     FNSWDLYNTDFPCKIKNDTLNWTKKIAFKNTLDLFSTSMPNCTPSIIKNFAVKLLCWI
                     EYFLPKLFYNKIKTNISPKVVYFGNIKRQELFFLYFLSQLGCDILYINPNKDILDLYP
                     ECSKFSTLFELSRKAPDILEIPFERHITNINYNKNTNKTSPNTNKPNSENTLNNANLT
                     SKKIVQNEEVELSYEDLAKLSASVVMIVVCNNENKPFKSGSGVVINNEGYILTNLHVV
                     NDGYSFLVRFENDDEVYTSYQIIKYHSDYDLAVIKVDRKCKPIPVKVSKKPVRGQKIV
                     AIGSPLGLFNTVSDGIISAFRDFETVQMIQFTAPISSGSSGGALLDMFGNLLGLISAG
                     YDDGQNLNLAVESSLVKIFANNFIEIVN"
BASE COUNT         2260 a          681 c          863 g         2103 t
ORIGIN      
        1 tagattattt actttttatt ataaggttca ttttttgaga ttagtatata ataaacatat
       61 agagtatata ataatagtta aagttaaaaa aggggaaaag caatatgcag tatatacaag
      121 aattacttga tttgactcaa aattattggg tgttagccac aataatcggc ttattaagtg
      181 catttataga aagctttata ccagccttac cacttgttgc tatagttaca gcaaatgctg
      241 caatacaagg attatttatt ggatgtttgt tatcatggat tggctcaggt cttgggacaa
      301 cctcattatt tttattaata agtagattta atgatagtaa gctttttaat aagttgagaa
      361 atagtaaaac tgaaaaagca ataagttgga tggataaaca gggatttaag ttattattta
      421 tagcgtatgc ctgtccattt atgcctggtt gtttagttac tatcgcatca gcttttggta
      481 aaaaagatgt aaaagatttt gtgccagcaa tgttggcagg aaaatttgta atgtttatag
      541 ttataagtta cgtggcaagt gatatagaag gttttataac aagtccttta aagatagtat
      601 catttatatt attggtgttt ttgtcatgga agattggaaa tagagtaaat aaaaatcttg
      661 aaaatcataa ttatgatttc catcataaaa aacatcacaa tgataaagat gataaaatga
      721 tttagcttgt acgaaataaa acgtatctta aaatgattta gtactaaatt attttaagat
      781 acgttttatt ttttgtatta aggacaaaat ttttagctct tttgtaaact aaaatatata
      841 agacttattt gtacttagtt caaaatattg ttatttattg tttctacaac tataataaaa
      901 tataaaattg atatccaatc aaaaaaataa ctatgaaatt taatagctta gatatgaatt
      961 tttctgtttt tgagttagtt tttataggta gtaaagaaat gtttatggta atgggataaa
     1021 acaattcaat accttcttta gtaataaggt caagacacaa gtgagataga tatcctatta
     1081 taaacccact ggacaaacat gagaaaacta tattattgtt agtgaagatt gtcagagatt
     1141 taaaaaaact ataaataagt aataaggcaa ttagactgtg agtaaatcct ctatgtctaa
     1201 acctgcttcc aaataatttg tatattaaca taaatttttt acttatgtaa gaacctctca
     1261 tatctatatc tggaaataaa gaacctaaat atgcaaaata aatataaact ataattaata
     1321 ctactttata tgcaatatcg tatttaacta ggtacagatt gtttataaag gggagtatca
     1381 aaagtgcaaa tatataacct cccctttcat gtgtttcttt tatcatataa aaatcctcct
     1441 gaatcgtcac tataaacatt atattggtaa atttagacta tcttttatta tattatatag
     1501 caaacataag tttttttcaa aaggatttta atatttaatt aatcttgaga ttttaaattt
     1561 ttgtaatttt ctaaagcacc attccaattt actacattaa accagttgtc aatgtattca
     1621 tttcttctat tttgatattt taaatagtaa gcatgctccc aaacatcaag tcctattata
     1681 ggagttaggt ttttacttac agggctatcc tgatttggag tagtcataat agataatttc
     1741 ccatctttag tagctacaag ccaagcccaa ccagaaccaa agacatctaa agcagatttt
     1801 tggaactctt gcttaaattt ttcaaaagaa ccaaagtctc tatcaatagc ttcttttaaa
     1861 gattcagaag gtatggtttt ttctggtgtc attatatcaa aaaagaattt atgattataa
     1921 gctccacctg cattatttct tacagttgta gcaatatctt taggtaaaga atctgaattt
     1981 tgcaataatt cacataaaga ataattataa agctcaggat atttttcaag agcagcattt
     2041 aatttatcaa cataagcttg ataatgctta tcatgatgca atttcattgt ttctttatct
     2101 atataaggtt caagtgcatc atatgcataa ggtaatggtt taaccttaaa tttgttgttt
     2161 tcaggtgtaa aagcaaacga agttatgcac tgtgagatta taagtaaaga cataataact
     2221 ggtattaata tttttttctt cataaaaatc acctcctaat atagtatttc tagaaataaa
     2281 aaaaatatga aatattaaca gataatttat aagaatttaa tagcaatttc attttaaatt
     2341 gaataagttt taaagccaaa gacaaaacta tgaaatatag aataagacta aaggacagaa
     2401 aggaattttg attatatgga tggtaatatt tcaatttatt ataaaggtga tatatctaaa
     2461 aaagaagata ttaaatttaa aataaaagaa tctgtaaata atacaagact ttctagtaga
     2521 atagagtata aggatgagga aaaaactttt gattttgaaa gatttaatgt aattgatgct
     2581 atttatggaa ataaaccatt tgaaaaagat ggaaaagtta ttgtttttaa tggaaatata
     2641 tataactatc atgaaataaa agaagaatta attaaaaaag gacacaattt tactacaaat
     2701 gatgatattg aagtcttatt ggcatcttat atagaatttg gtaagaattg tgtacataag
     2761 attaaaggaa tgtttaactt tataatctat gatagagaaa atgaaagtat atttggagca
     2821 agagatttat ttgggataaa accactttat tatataaata aggaaaatgc aataattttt
     2881 tcctcagagt ataagttttt acttgaatat atgaaaaatt taaatataaa tgaaagaagc
     2941 ttacaaagtt atttttcatt tcaatatgta cttcctgaag atactatgat acaaggaata
     3001 aggctgatac cagcaggtca ctattttagg gtagaaaatg gtatactatc tttaaagaga
     3061 tataataaac ttgagtttag aagtagtaca aaattttttt atactaaaaa tcacttaggc
     3121 aatagagatg ttaatgagga tgatataaga aatgtagtag tagattcgat aacaaatcat
     3181 atggaagagg acaaagaaat tggaacattt ctgtctggtg gaatagattc gtctattata
     3241 gccactgtgg cttcacaaat taatccaaat ataaaatctt tttctgcagg ctttagtgtc
     3301 aaaggatata gtgaattaga agtagcaaag aaaacagctg ataagttagg tatagaaaat
     3361 atccagatta atataacaca agatgaatat ataaaatcat taccgaatgt aatttattct
     3421 ttggatgacc ctatagcaga cccttcagaa gtaggtcttt atttcttaat taaagaggca
     3481 ggtaaacatg ttaaagtagt attgtctgga gaaggagcag atgaattatt tggaggatat
     3541 aatatatata aagagtatag tactatgaaa tcagttgtca attcacctac ttatattaaa
     3601 agtatgttag gaagagtttc agaacttatg ccaaacataa aaggaagaaa ctatctttat
     3661 agagctacaa caccattaga aaaaagatat ataggaaatg caaaagtttt tgaaaatagc
     3721 gaagtaaaaa gattcttttt taagtataag gaaaagaata tatatgaata cctgttgtct
     3781 aatttgtata gagatgcaca aaaaaataat tacgattata taagtaaaat gcaacacata
     3841 gatgtgaata catggcttca aggagatata cttcaaaaaa ttagtaaact ttctacagct
     3901 gagcaggttg aacttagagt tccattttta tataaagatg tatttgatgt agcaaaaaat
     3961 ttaagaatgg agcaaaaaat aaataagaat aatacaaaag ttttacttag ggatgcattt
     4021 agagaaatag tcccagaaca tgttgttcaa agaaaaaaat taggatttcc aactccaata
     4081 agagtttggt taaaagattc tctaggtgat attgtaaagg aaactatttc taattcaaat
     4141 gtagatgact ttatagataa aaaatatgta ataaaacttt tagatacaca tcttaaaggt
     4201 tatagagata attcgagaaa aatatggact atatttacat tttgtctatg gcatcaatta
     4261 tttattgagc ataaaaatgt cgagtattaa tactgttttc aatattaatc agagggcaaa
     4321 agcatgtaat gttttcgccc tctttattat tatggagtag gataagtaga attttgattt
     4381 attagtttac tatttcaata aaattattgg caaatatttt gacaagacta ctttcaacag
     4441 ctaaatttaa gttttgtcca tcatcatatc cagcggaaat caatcctagt aagtttccaa
     4501 acatatctaa aagagctcct cctgaactac cactagagat tggagctgta aattgtatca
     4561 tttgaaccgt ttcaaaatca cggaaagctg agataattcc atctgaaact gtattaaaaa
     4621 gacctaaagg gcttccaatt gcaactattt tttgacctct cacaggtttt ttactgactt
     4681 taacaggtat tggcttgcat tttctatcaa cctttatcac agctaaatca taatctgaat
     4741 ggtattttat aatttggtaa gatgtataaa cttcatcatc attttcaaat ctaactaaaa
     4801 atgaataacc atcattgact acatgaagat tcgtaagtat ataaccttca ttatttatta
     4861 ctactccaga accgctctta aacggtttat tttcgttatt gcatacaaca atcataacaa
     4921 cagaagctga taactttgca aggtcttcat aagaaagttc tacttcctca ttttgtacaa
     4981 tttttttaga tgttagattc gcgttattaa gtgtattttc agagttaggt ttatttgtat
     5041 taggagaagt cttattggta tttttattgt aatttatatt tgtaatatgc ctttcaaaag
     5101 gtatttctaa tatatctgga gctttcctag aaagttcaaa tagagtagaa aattttgaac
     5161 attcaggata taaatcaaga atatctttat ttggatttat atataaaata tcacacccaa
     5221 gttgtgataa aaaatataag aaaaaaagtt cttgtctttt aatatttcca aaatatacta
     5281 cctttggtga aatattagtc tttatcttgt tataaaataa tttaggtaga aaatattcta
     5341 tccaacacaa aagtttaact gcaaagtttt tgatgataga gggtgtacaa tttggcatac
     5401 ttgtggaaaa taaatctaaa gtatttttaa atgcaatctt tttagtccaa tttaaagtgt
     5461 cattttttat tttacatgga aagtcggtat tatataaatc ccaactgtta aacttagaac
     5521 tatcataaat attaaaaatt ttaagcatct tatctgtttg ttctttgtca aaatttattt
     5581 ttagattgct taatctaata tagggaattg tagtattatt aaaaaaataa gtatctatat
     5641 ttttaatttt ttctatgtat ttatcaattg aatcatcaac acctatatat cttgtaaagt
     5701 agacttcttt aatatatgaa gaactcttat ttttataggt agtatcagat ataaattcaa
     5761 ttagaggttc atttgacttt tttattgaaa tccttaatgg tttaatagta caaatcatat
     5821 tttttctcct ataataagat gatataatct attataaaac aacttaaaac aatatagtac
     5881 aataattttg tagtttcaaa ataaaat
//
LOCUS       DAEPXF010000212         5905 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain HB27
            SAMN13376389-rid12479423.denovo.220, whole genome shotgun sequence.
ACCESSION   DAEPXF010000212 DAEPXF010000000
VERSION     DAEPXF010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13376389
            Sequence Read Archive: SRR10518432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5905)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5905)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001069624.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001069624.1
            Genome Coverage       :: 315x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/15/2021 01:54:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,165
            CDSs (total)                      :: 4,076
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 89
            rRNAs                             :: 10, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 10 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 19 of 69
            Pseudo Genes (incomplete)         :: 44 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 10 of 69
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5905
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13376389-rid12479423.denovo.220"
                     /strain="HB27"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="China: Hebei"
                     /collection_date="2017"
     gene            complement(93..242)
                     /locus_tag="KRL67_003422"
     CDS             complement(93..242)
                     /locus_tag="KRL67_003422"
                     /inference="COORDINATES: protein motif:HMM:NF013677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HBH1524263.1"
                     /translation="MQKYSKELKLKAVNLYIKEGYSSYKIAEMLNIRSKTQVQNWVKD
                     YKNIG"
     gene            892..1626
                     /locus_tag="KRL67_003423"
     CDS             892..1626
                     /locus_tag="KRL67_003423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HBH1524264.1"
                     /translation="MNLTKGLDVLKISSNVLGEDKVMYIPVIYTEDEATLIDTGLPGQ
                     GDLIIDALNKSNTSFDKLKNIIITHHDIDHIGNINYLREKSKNSIKVYAYKSEVSYIT
                     GEETPFKLCMLEQMVDKIDDKMLSMLNVMGLGFKSSYTKVDVSLDNHEILNLGEKMEV
                     IHTGGHTRGHICLYLKESKILIAGDLLQIENGELKPVDVMHSNKQELKDAIKNISNYD
                     IETIVFSHGGLYQKNIIETLKNLIIE"
     gene            2610..3866
                     /locus_tag="KRL67_003424"
     CDS             2610..3866
                     /locus_tag="KRL67_003424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HBH1524265.1"
                     /translation="MIEKFKTLIWIKMIIRIKIVYKTYGGLFLLGLIGIPVGAIIGLI
                     DTIFGTVLLKVTDIRETYPMYLIPFLAVVGVVIAYCYFKFGGKSSKGMNLIFEVGHGE
                     EEIIPLRLVPFIISGTWLTHLFGGSAGREGVAVQIGATFSHWVGKRLPIKNASSIFLV
                     TGMAAGFAGLFETPIAAILFAMEVLVAGSLEYQSLFPAFTASFTASAVSKALGLEKFY
                     FTLSSKVVFDLSIFWKLIVLGIIFGMVGGAFAWCLKLAKRKIGNRLKNPMIRIAIIGV
                     CLSVLFLLFYKGRYSGLGTNLIQNSFYGGEIYSFDWLLKFILTILTLSAGFQGGEVTP
                     LFSIGASLGVLLAGFFNLPIELVAALGYASVFGSATNTFFAPVFIGAEVFGYSYLPYF
                     FVVCAISYIFNMDKSIYSLQKISTKQ"
     gene            3929..5122
                     /locus_tag="KRL67_003425"
     CDS             3929..5122
                     /locus_tag="KRL67_003425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HBH1524266.1"
                     /translation="MLTSLALIFLLGMASGGIFKKIKLPSLLGMLLTGIILGPYVLNL
                     IDNSILDISSDLRKIALIIILTRAGLSLDINDLKKVGRPAVLMCFIPATFEIIGMIVL
                     APKLLGVSILEAAVMGAVVGAVSPAIIVPKMLKLMEEGYGIEKSIPQMLLAGTSIDDI
                     FVIVMFTVFTGLAQGNSISAISFLQIPVSIILGVIAGAVIGLCLAVFFKNVHMRDSAK
                     GVLLLSISFLMISLETTLEGIVPFSGLLAVMSIGIFLQIKYRVVARRLSIKYSKLWVG
                     AEILLFVLVGATVDISYAFKAGIGAVILIFGVLLFRIVGVFFCLIKTNLTIKERLFCM
                     IGYIPKATVQAAIGGVPLAMGMVSGQLILTLAVLAILITAPLGAFGIDVTYKKLLTSV
                     KGESK"
     gene            5324..5704
                     /locus_tag="KRL67_003426"
     CDS             5324..5704
                     /locus_tag="KRL67_003426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HBH1524267.1"
                     /translation="MYLKKLSKLELVIMKFIWNLDIKTNSYEIIDYMKEEHNLPEKVA
                     LKTLSKLSKKRLLYVQETDKCMYYTVAIKERAYLEFISRNVQNLLKNNFIRNLLVSFH
                     EEELTEEKIKSLENWVVNWEEAYI"
BASE COUNT         2148 a          641 c          980 g         2136 t
ORIGIN      
        1 acgatttaat atattatcat atatctcata tgatttaaac ttacgtctat atactttctt
       61 tctaataata gatttaattc ctagttcttt catcaaccta tatttttata atcttttacc
      121 cagttttgaa cttgcgtttt gcttcttata ttcagcatct cagcaatttt gtatgatgag
      181 tatccttctt ttatatataa gtttacagct tttagtttaa gttctttgct atatttttgt
      241 attttttaga catagaaaaa ccaccttaga gtatacgctt tttagatcta ttataatggc
      301 cttttattta aatgtctaca ataaggggat tatactaaaa tagagataag cttctttttc
      361 attgctgaaa tgacgaaaat ataattgatt taatataaaa aagagtgctt tatgaactat
      421 ttattttgaa cagtagatac acttgcactc aatttagtta ctactaagct ttatgaacta
      481 aaagtttact ttgaatccta tttttattaa aagatatttt atcctttcta aataatcatc
      541 tatatacagt attgcgtttc ttttatttat tcataattta ttttcttatt aaagtttttc
      601 tatattcata ctttctatat tactttactt ttaactctat acgaattgtc aaaaataaaa
      661 tagttattag aaaaaaataa aagtaattgc aaagcttgca attacttact tatatactaa
      721 ttactatacc acaaaataac aaaaaaatca agtcgagtaa tccctcaaat taagtgatat
      781 actagatatt aataattttt aattaataaa atataactag aggcaaatct atatttctca
      841 atttatttaa ttaaattgac ctctaattaa gtaattataa ggagaaaaat tatgaattta
      901 acaaaggggt tagatgtttt aaaaatttca tcaaatgtat taggtgaaga taaagttatg
      961 tatattcctg taatctacac agaagatgag gcaacactta ttgacacagg tcttccaggt
     1021 caaggtgatt taataattga tgctcttaat aaaagtaaca cgagctttga taagttaaaa
     1081 aatataataa tcactcatca tgatatagac catataggaa acataaatta tctaagagaa
     1141 aaatctaaga atagtatcaa agtatatgca tacaaaagtg aagtcagtta tataacagga
     1201 gaagaaactc cttttaaact ttgtatgttg gaacaaatgg tagataagat tgatgataag
     1261 atgcttagta tgttaaatgt tatgggactt ggatttaaat catcatatac taaagtagac
     1321 gtgtcattag ataaccatga aattttgaat ctaggagaga aaatggaggt tatacataca
     1381 ggaggtcata ctagaggtca tatatgtctt tatttaaaag agtctaaaat acttattgct
     1441 ggagatttgc tacagataga gaatggagaa cttaagcctg ttgatgtaat gcacagcaac
     1501 aaacaagagt taaaagatgc tattaaaaat ataagtaatt atgatataga aacgatagtt
     1561 tttagtcatg gtgggttata tcaaaaaaac ataattgaaa cattgaaaaa cctaattatt
     1621 gagtagtact atatgattta caataatttc ttaaattact taaaattcta tctaaagatg
     1681 tagaatcaaa attaaatata aataataaga attttgtttt agaattatac ttaagggtta
     1741 agggtgatac gtagtattac aagtatttta gaatatagtt ttttatagga attttaaggt
     1801 tctacaatac ttcagttgaa aaatatttaa cagcatagta taataggaaa gaaaatattt
     1861 taaataaact ttagtataca aagtgaagaa aaatatttaa atcatatatc atgatgtaga
     1921 gtgaggtaaa atatttaaat catgtatcat aatacaaagt gaagaaaaat atttaaatca
     1981 tgtaccataa tgcagagtga gaaaaaatat ttaaatcata catcataatg caaagcgaag
     2041 aaaaatattt aaatcatgta tcataatgca aagttaagaa gattatttaa agcatgtatc
     2101 ataatacaaa gtaaagaaaa tcatttaaat tatatatcat aaaaataata tgaaatattg
     2161 aacactcata atattagagg ctttaatatt ttaattgata ggtcatatta tttataacat
     2221 taaaaataca atttacataa aatagacata tgaaaaataa aaaattatac aataattatg
     2281 ggaatgaggt tctcccttgg atgataatat cctaaactgc caatgagctg atgacttcta
     2341 tgatatcata gatgcaccag ctttttttgt tgtttaaatc gataaaatgg aggtgaatta
     2401 cttactaaat ttttattttt caaatttgaa gaaatatcta attaatagaa aaaatagtag
     2461 attatatgtt aattatttta ttaagaatag gttaagattg tacctatatc ctatcaattt
     2521 aaaaaacaat tagatagtca ttacattgac tacagttgat gcaataaaac tttcaaaaaa
     2581 tcttatgatg caaatagaaa ggaattaaaa tgatagaaaa atttaaaact ttaatatgga
     2641 ttaaaatgat aataagaatt aaaatcgttt ataaaacgta tggtggacta tttttgttag
     2701 gactcatagg tataccagtt ggtgcaatta taggtcttat agatacaata tttggaacgg
     2761 ttcttctaaa agttacagat attcgagaaa catatccaat gtatctgata ccatttttag
     2821 cagtagtagg agttgttatt gcttattgct attttaaatt tggaggaaaa agtagtaaag
     2881 gaatgaatct aatatttgag gtaggacatg gagaagaaga aattattcct ttaagattgg
     2941 ttccatttat catatcaggt acatggttaa ctcatttatt tggtggtagt gcaggacgag
     3001 aaggagttgc tgttcagatt ggtgcaacct tttcacattg ggtaggtaag aggttgccta
     3061 taaaaaatgc ttctagtatt tttctagtaa ctggtatggc agctggtttt gcaggattat
     3121 ttgagacacc aatagcagca atactatttg ctatggaagt tttggtagct ggttcactgg
     3181 agtatcaatc gctttttcct gcgtttactg cttcttttac agcaagtgca gtatcaaaag
     3241 ctttggggtt ggagaaattt tattttacac tttcttctaa agttgtattt gacttatcaa
     3301 tattttggaa gcttattgtt ttaggaatta tttttggaat ggttggtgga gcatttgcat
     3361 ggtgcctgaa attggcaaaa agaaaaatag gtaatagatt aaaaaatcct atgataagga
     3421 ttgctattat tggagtatgt cttagtgtat tattcctttt attttataaa ggaagatatt
     3481 caggtttagg gacaaattta attcaaaata gtttctatgg tggagaaatt tattcatttg
     3541 actggctgtt aaagtttata cttacaatac tgactttatc agcaggtttt caaggaggag
     3601 aggttacacc tttgttttcc attggagcta gtcttggtgt attgctagca ggatttttta
     3661 atttgccaat tgaattagtt gcagcgcttg gctatgctag cgtctttggc agtgctacaa
     3721 atactttttt tgctcctgta tttatagggg cagaagtttt tggatacagc tatttacctt
     3781 atttctttgt agtatgcgct atttcatata tatttaatat ggataaatct atttattcac
     3841 tacaaaaaat ttcaacgaaa caataaataa aacaacataa tgtatgatta gaagagaaag
     3901 aatattagag aaaggatagg gtaggaatat gttgacaagt ttagctttga tttttttatt
     3961 aggaatggcc tcgggaggta tttttaagaa gataaaatta cctagtttat tgggaatgtt
     4021 acttacagga atcatattag gtccatatgt tttaaatttg attgataatt caatactaga
     4081 tatttcttct gaccttagaa agattgcatt gattattata ttaacaaggg ctggattatc
     4141 tttagacata aacgatttga agaaggtggg aagacctgct gtacttatgt gctttattcc
     4201 agctactttt gaaattatcg gaatgatagt tttagcacca aaattattag gtgtttctat
     4261 attggaagca gcagtcatgg gtgcagtagt aggggcagtt tctcctgcaa ttattgtacc
     4321 taaaatgctt aaattgatgg aagaaggtta tggtatagaa aaaagtattc cacagatgct
     4381 tttagcagga acttctatag acgatatttt cgtaattgtg atgtttacag tattcacagg
     4441 attggcacaa ggaaatagta tatcagctat tagtttttta caaattccag tatcaattat
     4501 attaggtgta atagctggag cagtgattgg actctgttta gcagtatttt ttaagaatgt
     4561 tcatatgaga gattctgcta aaggggtttt gcttttaagt atctcatttt taatgataag
     4621 tttggagact actttagagg gtattgtgcc attttccgga ttattagcag ttatgagtat
     4681 tgggatattc cttcagataa aatatcgtgt agttgcaaga agattgtcta taaagtattc
     4741 aaaactatgg gttggtgctg aaattctctt gtttgttttg gtaggggcaa cagtagatat
     4801 aagttatgca tttaaagcag gaattggagc tgtaatttta atatttggag ttcttttatt
     4861 ccgtatagta ggagtattct tctgccttat taagacaaat ttgacaataa aagaaagatt
     4921 gttctgtatg attggctata ttccaaaggc tacagtacag gcagcaatag gaggtgtacc
     4981 acttgcaatg gggatggtat ctggacaact tattttgacg ttagcagttc tcgctatact
     5041 tataactgca cctcttggag cttttggaat tgatgtaact tataaaaaat tattaacttc
     5101 tgtaaaaggc gagagtaagt aaaaataaat attattttaa gtggccaatt atcttttgga
     5161 aaaggaatat actattgaat acaatataaa aaaatcattt gggagattaa agaatgtaga
     5221 agttaagaac aaattttaag gtatgttata attattagaa ttgattttgt gaaattatta
     5281 ttgtaatatt taattatata gataaagaag cggagtgata actatgtatt tgaaaaaatt
     5341 atcaaaatta gagttagtta ttatgaaatt tatttggaat ttagatataa aaaccaattc
     5401 atatgagatt attgattata tgaaagaaga gcataattta ccagagaaag tagctttaaa
     5461 aactttatcc aaattatcaa agaaaagatt attatatgtt caagaaacag acaaatgtat
     5521 gtattataca gttgcaatca aagaaagggc atatttagag tttatatcaa gaaatgtgca
     5581 aaatcttttg aagaataatt ttataaggaa tttattagta tcatttcacg aagaggagct
     5641 aacagaggaa aagataaagt cgttagaaaa ctgggtggta aattgggaag aagcttatat
     5701 ataaatgtta ttatttttat tacaatttag ataattttgg ttttataagc tctgcaacta
     5761 tttttagatg gttgcagagc ttatttttat tttttattcc cttaagaaat acttaaaagt
     5821 aaaagcattt atatttacta acttcaatta cttctaatac ttagattact ttttaaaaat
     5881 attttgatat gccatctcta tagta
//
LOCUS       DAEPXF010000213         5902 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain HB27
            SAMN13376389-rid12479423.denovo.221, whole genome shotgun sequence.
ACCESSION   DAEPXF010000213 DAEPXF010000000
VERSION     DAEPXF010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13376389
            Sequence Read Archive: SRR10518432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5902)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5902)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001069624.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001069624.1
            Genome Coverage       :: 315x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/15/2021 01:54:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,165
            CDSs (total)                      :: 4,076
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 89
            rRNAs                             :: 10, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 10 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 19 of 69
            Pseudo Genes (incomplete)         :: 44 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 10 of 69
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5902
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13376389-rid12479423.denovo.221"
                     /strain="HB27"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="China: Hebei"
                     /collection_date="2017"
     gene            130..1494
                     /locus_tag="KRL67_003427"
     CDS             130..1494
                     /locus_tag="KRL67_003427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HBH1524268.1"
                     /translation="MDIKELVKMQRKYYNTGKTRDISFRIEQLKKLKSVVSQNEEKIL
                     LALKKDLNKSDFEGFMTEVGMFYSELNFAIKNIRKWSKIKRVKSSMVNFPSISKVVPQ
                     PYGVTLIMSPWNYPFQLALIPLVWSLAAGNCVILKPSEYSVSTSSVVKDIVENTFSKE
                     YVAVVQGSQEESEKLLLERFDYIFFTGSTNVGKIIMKSASEHLTPITLELGGKSPCII
                     LRDANIDLTAKRLTWGKLINAGQTCVAPDYVLVHEDRKNELIEKIKYYTNKYFGDNPC
                     NNEQFPKIINQRHFNRILSLIDKDKIVYGGNYNKETLKIEPTIVDNVNWDDNIMKEEI
                     FGPIFPILTYEDLDEVIQKIIQMPNPLALYIFTKNKYLENKLLKMIPSGGCCINDTIT
                     HIATNYLPFGGIGESGMGSYHGKAGFDTFTHYKSVLKKLNLDVPIRYAPYDNKLIKFL
                     KKIM"
     gene            1632..2798
                     /locus_tag="KRL67_003428"
     CDS             1632..2798
                     /locus_tag="KRL67_003428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HBH1524269.1"
                     /translation="MKINWNTKYTTIAIYTFIIAASSIIFYLVSSQIDVFSNNLDAIF
                     TTLQPFIIGFAIAYLLNFILKFYEDRIFIKSEKLKKLKQSSKRGLGLLLTYATATLIL
                     YLFMHFVLPQVIESIVGLANDIPMYVNNATKLIDKLMTDLNLDEQYFNLAVDKWNEFV
                     TYIIKFVTDLIPILGSMLKNVASSIWNIVLGLIVSVYLLIDKEKFYGLSKKITYAVFT
                     EKQAARILELTHRSNYTFGRFLGGKILDSFIIGILTFVILTLVKMPYTLLISVIIGIT
                     NIIPFFGPLFGAIPSTLIVLFVSPIKAFWLLLIILIIQQIDGNIIGPKILGDSIGISA
                     FWILFSLLVAGKLLGFIGMVIGVPMFAVIYSIIKDIVESKLDKKGLPTDTSDYM"
     gene            2915..3697
                     /locus_tag="KRL67_003429"
     CDS             2915..3697
                     /locus_tag="KRL67_003429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
                     /protein_id="HBH1524270.1"
                     /translation="MAFIECKFKSKYTGGETDIIVILPVANGKELFSGKEIYDFEGKF
                     KTLYLFHGLEDDQSSWIRYTNVEHYAKNKNIALVIPRVETSFYTDMAYGHQYFTFVSE
                     ELPKFVRAIFPLSDKREDNFVAGMSMGGYGALKLALSKPKEFSAVASFSGSPDMIHEL
                     ENQQIETNADILFHDFGRPEDARNSENDLLYILKNLKERNEDIPKIYQFCGDRDFLYK
                     NNQMFKKYAESLGVDVEYEECVGGHEWRLWNLWIKKFIDIIG"
     gene            complement(3816..4469)
                     /locus_tag="KRL67_003430"
     CDS             complement(3816..4469)
                     /locus_tag="KRL67_003430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBH1524271.1"
                     /translation="MSKSEKGKETKASIISAARELFFEQGYHKTTTRQIAERANINLG
                     LISYYFSSKSEIGAIIYEDIRNEMQSLIYNYHEEGTMMMFFLAMNADLCFLLKNEAYR
                     NFYLDIAYEEAILSYQRSVTEMVMKRYVNDKESIEHDELILSCIGILAMKPEIIRVYT
                     SHKYDISSQAVIKHFTKQFLINLGHSTDLCEEILEELSKYYIDIVDNFTPIITRVRT"
     gene            complement(4493..5857)
                     /locus_tag="KRL67_003431"
     CDS             complement(4493..5857)
                     /locus_tag="KRL67_003431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter family protein"
                     /protein_id="HBH1524272.1"
                     /translation="MLLLIVTCAIYIWIGFYFSKKVKTASDFLIAGRNLPLPILAATI
                     AATSFGGGCLVGGVQWGAERGLWVGMYSTIGAALACFINAFFAGKLRSLSCDITPADY
                     IETRYGHSVFLRAYQSLVTPISILAIMGSQLISFGSVSTAFGIPYDIAVIIGVIVIII
                     YTYTSGMWGVAVSAFIQLAICIVFLPIVAYISLKLVGDNPLLMLQSMVKEPFFPGNKS
                     ISDFMYTVLPLVLGSIFSYESFLRYQCADNAKNAVKSSVIAGFILLFLAFPIGIIGAI
                     GGSLFPNISSVQVLPQMISTTLPPLISVLFLAAILAAIMSTADSLMTSMSAIISRDIY
                     NKLLHPDIEFNDLKNTLKYSQIASALTAIIGAVIALKFDSLLELLFWPAPLCTGVIFA
                     PFVIGLCWKGATKKGAIYAVIVSVILALLNMIGILTVFDRILVPIIGGSITIFIVSKF
                     SKKD"
BASE COUNT         2221 a          755 c          899 g         2027 t
ORIGIN      
        1 ctttttttat tggaagattt atgttatatt gagatgaatt tattatgata taaaaaataa
       61 tgttaggata aatttaaatc tttttctaaa aatgtattag tataattctt aaagaaagga
      121 tttttaacaa tggatataaa agagttagtt aaaatgcaaa gaaaatatta taatacaggt
      181 aaaacaagag atatatcgtt tcgtatagaa cagttgaaga aacttaagtc agttgtatca
      241 caaaatgaag aaaaaatact tttagcacta aagaaagatt taaataaatc ggattttgaa
      301 ggttttatga cagaggtagg catgttttat agtgaattaa actttgctat aaagaatata
      361 agaaaatgga gcaaaataaa aagagttaaa tcaagtatgg ttaattttcc atctatatcc
      421 aaagttgttc cacaacctta tggtgttact ctcataatgt caccttggaa ttatccattt
      481 caattagcac ttataccatt agtttggtca ttagcagcag ggaattgtgt tatattaaaa
      541 ccttctgaat attctgtgtc aacatctagt gtagttaaag acatagttga aaatactttt
      601 tcaaaagaat atgttgcagt tgttcaagga agtcaagaag aaagtgaaaa gttactattg
      661 gaaagatttg attatatatt ttttacagga agtactaatg ttggtaaaat aatcatgaaa
      721 tcagcatcag agcatttaac tcctattact ttagaacttg gaggaaaaag tccttgtatc
      781 attttaagag atgctaatat agaccttact gccaaaagac ttacttgggg aaaactaata
      841 aatgcaggac aaacctgcgt ggctccagat tatgttttag tgcatgaaga tagaaaaaat
      901 gagttaatag agaaaataaa gtattatact aataagtatt ttggagataa tccatgtaat
      961 aatgagcaat ttcctaaaat cataaatcag agacatttta atcggatatt atctcttatt
     1021 gataaagata agatagtcta tggtggaaat tataataagg agacactaaa gatagagcca
     1081 actatagtag ataatgttaa ttgggatgat aatataatga aggaagaaat atttgggcct
     1141 atatttccca ttttaacata tgaagatttg gatgaagtaa tacaaaaaat tatacaaatg
     1201 ccaaatccat tagcacttta tatttttaca aaaaataagt acctagaaaa taaactttta
     1261 aaaatgattc catcaggtgg atgttgtata aatgatacaa ttactcatat agctacgaat
     1321 tacttgcctt ttggtggtat tggtgaaagt ggtatgggtt catatcatgg aaaggctgga
     1381 ttcgatacat ttacgcacta taagagcgta ttaaaaaaat taaatttgga tgtccctatt
     1441 agatatgctc catatgataa taaattaatt aaatttctta aaaagataat gtagttgtat
     1501 tatggaaaaa taaatataaa taaaattgag catttgtatt ctgaatatat tttattttta
     1561 atttggtata tttgttataa aagtggtgat tttgtgataa aatttaatta tatagaaata
     1621 aggaggaaaa aatgaagatt aattggaata caaaatacac aacaatagca atttatacat
     1681 ttataatagc tgctagtagt attatatttt atctagtatc atcacaaatt gatgtatttt
     1741 ctaataattt agatgctata tttacaacat tacaaccttt tataatagga tttgctattg
     1801 catatcttct taattttatt ttaaagtttt atgaagacag aatttttata aaaagtgaga
     1861 aattaaaaaa actaaaacaa agttcaaaaa gaggcttggg tcttttatta acatatgcaa
     1921 cagctacatt gatactttac ttatttatgc atttcgtatt gccacaagtg attgagagta
     1981 ttgttggact agcaaatgat atacctatgt atgtaaacaa tgcaactaag cttattgata
     2041 aattaatgac tgatttaaat cttgatgagc aatactttaa tttggcagtt gataagtgga
     2101 atgagtttgt tacatatatt ataaagtttg taacagattt aatcccaata ttaggaagta
     2161 tgttaaagaa tgtagcctct agtatatgga atattgtact aggattaatt gtttcagtat
     2221 acttattaat tgataaagaa aaattctatg gattaagcaa aaaaataact tatgcagtat
     2281 ttacagaaaa acaagctgcc agaattttag aattaactca tagaagtaac tatacatttg
     2341 gaagattttt aggtggcaaa atattagatt cattcattat aggtattcta acatttgtaa
     2401 tacttacatt agttaaaatg ccatatacat tattaatatc agttataatt ggtattacaa
     2461 atattatccc tttctttgga cctttatttg gagctatacc atctacacta atagtgctgt
     2521 ttgtatctcc tataaaagct ttctggttat tgttaattat attaataata caacagatag
     2581 atggaaatat tataggtcct aaaatacttg gagattcaat tggtatatct gcattttgga
     2641 ttttgttctc cttattggtt gcaggaaaat tattaggttt tattggaatg gttataggag
     2701 ttccaatgtt tgcagtaatt tattctataa ttaaggatat tgttgaaagt aaattggaca
     2761 aaaagggatt accaacagat acttccgatt atatgtagct aaaaaatatt atgtataatt
     2821 aaaaatatat aaattaagca atatttaaag cactgagaaa gttaaactct tggtgctttt
     2881 tagatataaa attattatag aagaggtgtt ttttgtggca tttatagaat gtaaatttaa
     2941 atctaaatat actggaggag aaactgatat aattgtaatt ttgcctgtag caaatggaaa
     3001 agaacttttt agtggtaaag agatatatga ttttgaaggt aaatttaaaa cattatactt
     3061 atttcatggt ttagaagatg accaaagctc atggattaga tacactaatg tagaacatta
     3121 tgctaaaaat aaaaatatag ccctagtaat accaagagta gaaactagtt tttatacaga
     3181 tatggcgtat ggacaccaat attttacttt tgtaagcgag gaattaccta aatttgttag
     3241 agctatattt cctttatcag ataaaaggga ggataacttt gtagctggta tgtctatggg
     3301 gggatatgga gctttaaaat tagcactttc aaagccaaaa gagttttcgg cagttgcaag
     3361 tttttctggt tcgcctgata tgattcatga gttagaaaat caacaaatag aaaccaatgc
     3421 agacattcta tttcacgatt ttggtagacc agaagacgct agaaatagtg agaatgattt
     3481 actttacata cttaagaacc ttaaagaaag aaatgaagat attcctaaaa tttatcaatt
     3541 ttgtggtgat agagattttt tatataaaaa taatcagatg tttaaaaaat atgcagaatc
     3601 tctaggtgta gatgttgaat atgaagagtg tgtaggtggg catgaatggc gtctttggaa
     3661 tttatggata aagaaattta tagatataat tgggtagtac acttatggta ttaacagaga
     3721 tattataaaa tatctttaaa tatgataaag actgaaatcc taaaatcaaa ttatgatttt
     3781 aaaatttcag tctctaagaa tttaataaaa ttttattaag ttcttacacg agttattatt
     3841 ggagtaaaat tgtcaactat atctatatag tatttactca attcctctaa aatctcttcg
     3901 cataaatccg tactatgacc aagattaata agaaactgtt ttgtaaaatg ctttattaca
     3961 gcttgagaag atatatcgta cttgtgtgaa gtataaactc taattatttc aggtttcata
     4021 gcaagtatac caatacaaga aagtattaac tcatcatgtt ctatagattc tttatcatta
     4081 acatatcttt tcatgaccat ttcggttaca cttctttgat agcttaaaat agcttcttca
     4141 taagctatat ccaaataaaa atttctatat gcttcgtttt ttagaaggaa acaaaggtca
     4201 gcattcatag caagaaagaa catcatcata gtaccttctt cgtgatagtt ataaattaaa
     4261 gactgcattt catttcttat atcttcatat attatagctc ctatttcgct tttacttgag
     4321 aagtaatagc ttattagacc taaatttata ttagctcttt ctgcaatttg cctagttgtt
     4381 gtcttatgat aaccttgctc aaaaaataat tctctagcag cacttataat actagctttt
     4441 gtttctttgc ccttttctga ttttgacata tttactgtgt atacctctct tttcaatctt
     4501 tttttgaaaa tttgctaact ataaatattg taatagaacc acctattata ggaaccaaaa
     4561 ttctatcaaa tacagtaaga ataccaatca tatttaataa tgccaatata acacttacta
     4621 taacagcata gatagctcct ttttttgtag cacctttcca gcaaagacca attacaaatg
     4681 gtgcaaatat tacacctgta catagtggag ccggccaaaa caataattct aataaactat
     4741 caaatttaag tgctatgact gcacctatta tagctgtcaa agctgatgca atttgagaat
     4801 actttagagt gttttttaaa tcattaaatt ctatatcagg gtgtaacaat ttgttataaa
     4861 tatctcttga tataatagca ctcattgagg tcattaatga atcggcagta gacattatgg
     4921 cagccaatat agcagctaag aataaaacag agattaaagg tggcaaagta gtcgatatca
     4981 tttgaggtaa aacctgaaca gatgatatgt ttggaaataa actaccacca atagctccaa
     5041 ttattcctat agggaaagca agaaataata gtataaatcc tgcaattaca ctacttttaa
     5101 ctgcattttt agcattatca gcacattgat atcttaaaaa gctttcataa gaaaatattg
     5161 agcctaaaac taatggtaaa actgtataca taaaatcact aatcgattta tttcctggaa
     5221 agaatggttc ttttaccatt gattgtaaca taagtaaagg gttgtcgcca actaatttta
     5281 agcttatata agctactatt ggcaaaaaca ctatacaaat agcaagttga ataaatgctg
     5341 atactgcaac tccccacatt cctgatgtat aagtatatat aatgataact ataactccta
     5401 taattacagc tatatcataa ggaataccaa atgcagtaga tacagaacca aaagaaatta
     5461 attgactacc cataatagct agtatagata ttggtgttac aagactttga taagctctta
     5521 aaaatacact atgaccatat ctagtttcaa tataatcagc aggtgttata tcacaagata
     5581 agcttcttaa ttttcctgcg aaaaatgcat ttataaagca agctaaagct gcacctatag
     5641 tagaatacat tccaacccaa agtcctcttt cagctcccca ttgaactcca ccaactaagc
     5701 accctcctcc aaaagatgta gcagcaatgg ttgcagctaa tataggcaaa ggaagatttc
     5761 tacctgcaat taggaaatcg gatgctgttt taactttttt tgaaaaataa aaaccaatcc
     5821 atatgtatat tgcacacgta acaattaaaa gtaacatagt ctcttctcct ttataaaata
     5881 ttttaataaa atatataaaa ta
//
LOCUS       DAEPXF010000214         5894 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain HB27
            SAMN13376389-rid12479423.denovo.222, whole genome shotgun sequence.
ACCESSION   DAEPXF010000214 DAEPXF010000000
VERSION     DAEPXF010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13376389
            Sequence Read Archive: SRR10518432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5894)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5894)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001069624.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001069624.1
            Genome Coverage       :: 315x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/15/2021 01:54:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,165
            CDSs (total)                      :: 4,076
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 89
            rRNAs                             :: 10, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 10 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 19 of 69
            Pseudo Genes (incomplete)         :: 44 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 10 of 69
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5894
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13376389-rid12479423.denovo.222"
                     /strain="HB27"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="China: Hebei"
                     /collection_date="2017"
     gene            302..736
                     /locus_tag="KRL67_003432"
     CDS             302..736
                     /locus_tag="KRL67_003432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH1524273.1"
                     /translation="MESMNSFKKIEIEHVRIRVQLEKNNPSTALYISLLAVLFSFLYN
                     FAFETIKTTLNGLITVLKIENLDINLIANVICFVSFLFFVQNMSSKIGSLCRNDIQFY
                     ITALDLLEDMKKKNLEKESIKKEDNKEIFITFFFYTLKFNCI"
     gene            complement(754..1074)
                     /locus_tag="KRL67_003433"
     CDS             complement(754..1074)
                     /locus_tag="KRL67_003433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH1524274.1"
                     /translation="MNELVRHRFKIYLCLKKIISSVVILLGNYEKSINNSIIYGNKIV
                     SSIKDLLKISQLTKEYNSAMSTFLLTDLDYKYIKEMMLKFNLLDDELSELESTVKEIM
                     LDSE"
     gene            complement(1121..1315)
                     /locus_tag="KRL67_003434"
     CDS             complement(1121..1315)
                     /locus_tag="KRL67_003434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH1524275.1"
                     /translation="MNHYHPKSKYPYEVLVWENLLFSCGMCNNKKRAYDTDLEPIINP
                     TEYKPKEILTLAKFFICKQK"
     gene            complement(1473..1637)
                     /locus_tag="KRL67_003435"
     CDS             complement(1473..1637)
                     /locus_tag="KRL67_003435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH1524276.1"
                     /translation="MGIERNFLKLYLKVIINFEDAEKVKKNYEILDKMLHPKNHLRKM
                     LQIQMAGFMK"
     gene            2106..2288
                     /locus_tag="KRL67_003436"
     CDS             2106..2288
                     /locus_tag="KRL67_003436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HBH1524277.1"
                     /translation="MFKFYNNATLMFLSGTNIKTISERLGHTDIKITMNRYSQVLEEM
                     DREAFENLSKILFIVF"
     gene            2359..3204
                     /locus_tag="KRL67_003437"
     CDS             2359..3204
                     /locus_tag="KRL67_003437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH1524278.1"
                     /translation="MNIIKNITLLLTLLIVILMTGCSPRESASEVAKKYIDKMNSGDY
                     ETAYSLLSKDSKKKISIENLEEFQNILVSTKRIISYKIGKEKIIKKYKHDGKEYKNVV
                     KLEETFNIKNLLYKKDDSLKVNRYFVVENNKYKLLLYESFKKEFGINSMDLAQLKLND
                     FGLKYFKEVDLILKKAIAINPTDSKLYYAQACNYMNFDGFPGYSDTTYNALDSINNSL
                     KYLDKNDSDYKKNASNIYNLKGVILMFHKRFDEAQQYLNKALYFNKDNEDPKYNLNTI
                     KNHLN"
     gene            complement(3428..3592)
                     /locus_tag="KRL67_003438"
     CDS             complement(3428..3592)
                     /locus_tag="KRL67_003438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH1524279.1"
                     /translation="MFGKPKESVRFLDFNDVAEKFGEKKAVAIFDAIAGKKSKEHIIK
                     DIEKLVKNEV"
     gene            complement(4127..4459)
                     /locus_tag="KRL67_003439"
     CDS             complement(4127..4459)
                     /locus_tag="KRL67_003439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HBH1524280.1"
                     /translation="MNTREMKEYRGFYNKKLSETDEFFRVFGSLDDKTYSEGAISKKN
                     KELMGLAISILSRCDECICYHIEGCINSGASKDEIIEAIKIGVIGGGSITYPNARFAI
                     KILEEFNI"
     gene            complement(4703..5344)
                     /locus_tag="KRL67_003440"
     CDS             complement(4703..5344)
                     /locus_tag="KRL67_003440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HBH1524281.1"
                     /translation="MKKAINKQFILSTLICFIPFIVSIYFYNRLPNEVAIHFDNYGNP
                     DNYAPKVIAAFGVPLLMLCIHLYTWFRLENEPKKANFSNVIQNLSKWGVAILSVILQI
                     MMVLYSTGVSLESNFYTGLILGIIVILFGNYLPKCKQNYSVGMILPWTLNDEDNWNKT
                     HHLAGWIWLIGGILLIINAFINIPFYNIFVIFVIVILPFIYSYLMYKISGKNN"
     gene            complement(5325..5618)
                     /locus_tag="KRL67_003441"
     CDS             complement(5325..5618)
                     /locus_tag="KRL67_003441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HBH1524282.1"
                     /translation="MGFPETFKALSDPIRREILVMLRENKMSAGEISEKFNMTNATIS
                     YHLSQLKKAGLIFETKYKNFIFYEINISVFEEVMLWFSQFMEKGEKDNEKSDK"
BASE COUNT         2236 a          807 c          558 g         2293 t
ORIGIN      
        1 aaatttaaaa cttatagctt gtttttacaa actctctagc cttatccatt gcaggccata
       61 catcattgct catatatttg agtaagtttt tctcaactag ctctatttta tctaaaatcg
      121 ttcgacaaat tgctaatata agttcttaaa tatgctataa attaaagtat attatatata
      181 ctcggagtat tttatgattt tagtaattat aaatttagtg aactagtttc aaaatctgaa
      241 agataaaaaa tattttaaga aataccataa atattacaca aaaatctatt attataatga
      301 aatggaaagt atgaatagtt ttaaaaaaat agaaattgaa catgtcagaa ttagagttca
      361 gttagaaaaa aataatcctt ctactgcatt gtacatatct cttctagctg tacttttttc
      421 atttttatat aattttgcat ttgaaactat aaaaacaact ttaaatggtt tgataactgt
      481 tttaaaaatt gaaaatttag atataaattt aatagctaat gttatatgtt ttgtttcttt
      541 tttgtttttc gtacaaaata tgtcatctaa gataggatct ttatgtagaa atgacataca
      601 attttatata actgcactag atttattaga agatatgaaa aagaaaaact tagagaaaga
      661 aagtataaaa aaagaagata ataaagaaat ttttattacc ttcttttttt atactctcaa
      721 atttaattgt atctagttat tttaatatgt atttcattct gaatctaaca ttatttcttt
      781 tactgtactt tctaattcac ttaattcatc atccaataaa ttaaacttta acatcatttc
      841 ttttatgtat ttataatcta aatctgttaa caaaaatgtt gacatagcag agttgtattc
      901 ttttgttagt tgagatattt ttaataaatc tttaattgaa cttacaattt tatttccata
      961 aattattgaa ttattaatac ttttttcata atttcctaat aaaataacaa cagaagaaat
     1021 tatctttttt aaacataggt atattttaaa tctgtgccta actaattcat tcacattatt
     1081 taattctaat acatcaatag tacactcgcc taattaattt ctatttttgc ttacatataa
     1141 aaaatttcgc aagtgtaagt atttctttag gcttgtactc tgttggattt ataattggct
     1201 ctaaatcagt atcatatgca cgttttttat tattacacat tccacaagaa aacaataaat
     1261 tctcccatac taatacttca tatggatact tacttttagg atgataatga ttcacttaca
     1321 tgtatttgcc ttcttccatt aatttagttt cacagtagca acattttcca ttagatatga
     1381 caaaaagtgt attttttata tattttttat tccatacgtt tttttatact cttctgttaa
     1441 ttgtttggat ttaggttttc tttctatttt tatcatttca taaaacctgc catttgtatt
     1501 tgcaacattt ttctaagatg atttttagga tgtaacattt tatctaaaat ttcataattc
     1561 ttttttactt tttcagcatc ttcaaaattt ataatcactt tcagatataa ttttaaaaaa
     1621 ttacgttcta tacccataac atcttttaat atttcttcat tagtccagcc taataaccca
     1681 tattctccaa gttataactc ttttttatat acattaccac tttcatctat atctaacata
     1741 attatttctt ctttagaatc aatttgtaaa atatgaggac tgtgtgttgt gacaattatt
     1801 tgtgttttta ggagaattgc ttttaatgta ataaaaattt tacataccac atagttaata
     1861 taaaacctca aaataaactt agcatctcca atgtttacac atatacaatg cttttaaaat
     1921 tgcagtgagc gtgcaatagt gcaattgata atacttatca cacattctca atgccttata
     1981 tttcaactgt taaacctcaa atttgtcaca aatatcgctc actgcaaaat ttctatattt
     2041 ttattatatc ataacatata attaatattt tcaattaatt tttaactgta tttttacatt
     2101 taattatgtt taaattctat aataatgcaa ctttaatgtt tctttctgga actaatataa
     2161 aaactatttc agaaagatta ggtcatacag atataaaaat aactatgaat agatactctc
     2221 aggttttaga agaaatggac agagaagcat ttgaaaattt gagcaaaata ctatttattg
     2281 tattttaaag aagatgactt attcaatatt tgatgctaga atataattaa taaaaattta
     2341 attataggtg attgtattat gaatattatt aaaaacataa ctttactctt aacattatta
     2401 attgtgatat taatgacagg atgttcccca agagaaagtg ccagtgaagt tgcaaaaaaa
     2461 tatattgata agatgaactc tggtgactat gaaacagcat atagtttatt aagtaaagat
     2521 agtaaaaaaa aaattagtat agaaaatctt gaagaatttc aaaatatttt agttagtaca
     2581 aaaagaataa ttagttataa aataggtaaa gaaaaaatca taaaaaaata taaacatgat
     2641 ggaaaagaat ataaaaatgt tgttaaatta gaagaaactt ttaatataaa aaaccttctt
     2701 tataaaaaag atgattctct aaaagtaaat agatattttg tagttgaaaa taacaaatac
     2761 aagctattat tgtatgaaag tttcaaaaaa gaatttggta ttaatagtat ggaccttgct
     2821 caacttaagc ttaatgattt tggactaaaa tactttaaag aagttgacct tatactaaaa
     2881 aaagctattg ctattaatcc tactgattca aagctttatt atgcccaagc atgtaattat
     2941 atgaattttg atggatttcc tggttattca gatacaacat ataatgcttt agattcaatt
     3001 aataattctt taaagtattt agataaaaat gattctgact acaaaaaaaa tgcaagtaat
     3061 atatataact taaaaggagt tattcttatg tttcacaaaa gatttgatga agcacaacaa
     3121 tacttaaata aagccttata ctttaacaaa gataatgaag acccaaagta taatctaaat
     3181 acaataaaaa accatctaaa ctagatattt aatttttgaa tctataaaat atttttttat
     3241 atataattca gtactgaacc acaatctcta ctaaactaat tactagttta aattataaag
     3301 acttatttct atataaatat tactctataa caataagttt cttatttaaa ttttaaaata
     3361 ttatctaaac atcataaaaa agaaggtata aagagctacc ctcaagctca atatacctta
     3421 taaatatcta aacttcattt tttactaatt tttctatatc tttgataata tgttctttag
     3481 atttttttcc agctatggca tcaaaaattg caacagcctt tttctctcca aacttttctg
     3541 ctacatcatt aaagtctaaa aatctaacac tttcttttgg tttaccaaac atataacacc
     3601 tctcatacat ttttatatat ttattataac ttctatagac ttatttttca ccaataaacg
     3661 ttagacaaat tagtcccttt atttttttaa actagcttta ccttcaaaat tcttaacaaa
     3721 aacagtttat tctactctat aaataaaaat agacaattta atttgtagga aactgataat
     3781 agaatacagt aaataaaact ttgtttaatc tatctgaaaa agttatctat taaaactact
     3841 atgaatttat aaagaatatt ctagttcata aaaatacaaa tatgtaaata taattacctt
     3901 aatattcaat ttatttaaaa taaaaatgta caaaagattt aataataaca atatactatt
     3961 ctcaatacaa tctatgatgt aaataattta aaactactat attgttttaa aatttatatg
     4021 ttcaattcta aaaacttaat aagaagtgaa cttagaaaat aaagcttaat ttcaaagact
     4081 taaatagact attattatta gtctaaataa actacttcta tcaaaattat atattaaatt
     4141 cttctaaaat ttttattgca aatcttgcgt ttggatatgt aatagacccg ccaccaatta
     4201 cacctatttt tatagcttct ataatttcat ctttacttgc tccagaatta atacaaccct
     4261 caatatgata acaaatacat tcatcacatc ggcttaaaat tgaaatagct agtcccatta
     4321 attccttatt ttttttactt atagcccctt ctgaataagt cttatcatca agacttccaa
     4381 atactctaaa aaactcatct gtttcgctta attttttatt gtaaaatccc ctatattctt
     4441 tcatttctct agtattcatc ttctacctcc tatgtttttc ttataagaat atcacactgt
     4501 acttatattt tcaatattaa atacaaattt tcatattatg ttcattagcc taatttatat
     4561 tattttttga aatctcaaca agttatcata taagtaagat tttttcagaa ctaaaataga
     4621 aaccttagta atttctttat tatctatctc agttctgaaa tatattattc ttatccaaat
     4681 attattatag tgattaatta tattagttat ttttacctga aattttatac atcaaataag
     4741 aatatataaa tggtagtatt acaattacaa agattacaaa gatattataa aaaggaatat
     4801 taataaatgc attaataatt aataatattc caccaataag ccaaatccat ccagctaaat
     4861 ggtgcgtctt attccaatta tcttcatcat taagagtcca tggtaaaatc attcctactg
     4921 agtaattttg tttacattta ggaagatagt ttccaaataa aataactatt attcctaaaa
     4981 ttaatcctgt ataaaaatta gactccaaac taactcctgt tgaatataaa accatcatta
     5041 tttgtagaat tactgaaagt atagcaactc cccattttga taaattttgt ataacatttg
     5101 aaaaattagc tttcttaggc tcattctcaa gtctaaacca tgtatacaaa tggatacata
     5161 gcattaacaa tggtacacca aaagctgcta taacctttgg agcataatta tctggatttc
     5221 cataattgtc aaaatgaatt gcaacttcat ttggtaatct attataaaaa tatattgata
     5281 caataaatgg tataaaacat attaaagtag atagaataaa ttgtttattt atcgcttttt
     5341 tcattatctt tttctccctt ctccataaat tgtgaaaacc aaagcataac ctcttcgaaa
     5401 actgaaatat ttatttcgta gaaaataaag ttcttatact ttgtttcaaa tatgagtcct
     5461 gcctttttta gttgagatag atgataagaa attgtagcat tcgtcatatt aaatttttca
     5521 ctaatttctc ctgctgacat tttattctcc ctcaacataa ccaatatttc tcttcttata
     5581 gggtctgaaa gagctttgaa tgtttctgga aaccccaaca ccatcacctc gctatttaga
     5641 atttcatcta aatagaatat aacataacat ccattttatt tcaatagcta tttagaaaaa
     5701 tttcgaaata gctatttcta gatacttatt taaaattttt atattaagat tctcaattta
     5761 cttaagtcgc ttttatttaa ttcttatttt atctaatttt tttaatttaa caaaagtgta
     5821 ttacaccaca attgttaaag taaaaaccta atgactgaga tacttatata tattacatat
     5881 acttttaatt tcaa
//
LOCUS       DAEPXF010000215         5887 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain HB27
            SAMN13376389-rid12479423.denovo.223, whole genome shotgun sequence.
ACCESSION   DAEPXF010000215 DAEPXF010000000
VERSION     DAEPXF010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13376389
            Sequence Read Archive: SRR10518432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5887)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5887)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001069624.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001069624.1
            Genome Coverage       :: 315x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/15/2021 01:54:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,165
            CDSs (total)                      :: 4,076
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 89
            rRNAs                             :: 10, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 10 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 19 of 69
            Pseudo Genes (incomplete)         :: 44 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 10 of 69
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5887
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13376389-rid12479423.denovo.223"
                     /strain="HB27"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="China: Hebei"
                     /collection_date="2017"
     gene            173..862
                     /locus_tag="KRL67_003442"
     CDS             173..862
                     /locus_tag="KRL67_003442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH1524283.1"
                     /translation="MLQKVLWKFLKLVVGLFICSVGIVLTINCNLGLSPWDVFHQGLS
                     NHIGITIGTASIIVGSIVVIADVVLGENVGWGTVFNMLLIGFFMDLLLYSNLIPEADS
                     LFVGIIMLILGLVLLSVGMVFYMGSGLGSGPRDGLMVALQKKTGKSTNLIRGTIEVGA
                     LIVGFLLGGKVGIGTIISAFGLGYFTQMVFGLFKLDCSKIKHRFIVDDIKFIKVYMSG
                     EKKAAVQNTVK"
     gene            1297..1836
                     /locus_tag="KRL67_003443"
     CDS             1297..1836
                     /locus_tag="KRL67_003443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007286148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBH1524284.1"
                     /translation="MDIGEKIKRLRTEKQLTQEELANRCELSKGFISQLENNLTSPSI
                     ATLIDILEILGTNLREFFNEIDDERISFTKEDMFETEDEDLKYKLKWLIPNSQKNEME
                     PIIITLYPGGQYKEEKPHEGEEFGYVLAGSIYVHIGEKKNKVKKGESFYFRPKANHYI
                     SNEGKTTAKVIWVSTPPSF"
     gene            1855..2898
                     /locus_tag="KRL67_003444"
     CDS             1855..2898
                     /locus_tag="KRL67_003444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011721317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBH1524285.1"
                     /translation="MVENIIELRDISKHYEELTVLDNFNLDIKKNEFLTLLGPSGCGK
                     TTTLKIIAGFEYADDGKVLFEGKEINNLPPYERQVNTVFQKYALFPHMDVYENVAFGL
                     KIKKTPKDVIDAKVKEVLKLVALEGFERRQVESLSGGQQQRIAIARALVNEPKVLLLD
                     EPLGALDLKLRQEMQIELKRIQKKLGITFVFVTHDQEEALTMSDTIVVMYKGKIQQMG
                     TPQDIYNEPKNSFVAKFIGESNIFDGIMIEDYKVNFCNRDFECVDKGFEKNENIEVVI
                     RPEDIKMVKPEEGMLKGKVTSTVFKGVHYEIELNENGRLWILHNTKNAEVGTELGMDI
                     YPEDIHIMRKESN"
     gene            2900..3736
                     /locus_tag="KRL67_003445"
     CDS             2900..3736
                     /locus_tag="KRL67_003445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBH1524286.1"
                     /translation="MKRKSFLAYPYVVWSAIFVIIPLILVVFFSFTKESGGGYAFTLE
                     NYKEVIDPIYMKVFGRSILLAGGATLICLIVGYPVAYIISKARVSRRGSLILLFILPM
                     WMNFLLRTYAWVAILGKNGLLNTFLGWFGIQPLAILYTNFAILLGMVYNFLPFMVLPI
                     YTALSKMDNDLINAAHDLGANNMTVFRKIIFPLSLPGVMSGITMVFMPAVTTFAISRL
                     LGGGKIMLVGDLIEQQFTVVGDWNFGSAISIFMMIVILISMSIMSKFGDESDKEGGGL
                     LF"
     gene            3841..4515
                     /locus_tag="KRL67_003446"
     CDS             3841..4515
                     /locus_tag="KRL67_003446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBH1524287.1"
                     /translation="MARWNGFTWKWYGQLLQNESIMSALYYTIVIAILASVISTIVGT
                     ISAIGIHKMRGKSKKLILNVNYLPILNTEIVTAVALMSLFVFVKMEFGFTTMLLAHIM
                     FCLPYVILSVLPKMKQLPDNIEDAAMDLGATPIYALRKVILPQIKPGIVSGFLIAFTM
                     SIDDFIISFFNAGNGVSNLSIEIYGMARRGIKPEINALSTIMFAVVLGLLLLANKKES
                     IVRGTK"
     gene            4515..5567
                     /locus_tag="KRL67_003447"
     CDS             4515..5567
                     /locus_tag="KRL67_003447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein"
                     /protein_id="HBH1524288.1"
                     /translation="MIKFKKVISLIAVGMMTVTLFTGCNKATDSTKVLNVYNVGDYID
                     ESLIDKFEKETGIDVQYSTYDTNEMMYQKVKSGSTHYDLVFPSDYMVEKMKNEGLLEK
                     LDFKNIPNMKYLDKSFLNPVYDKTNEYSVAYMWGTLGILYNKKEVKDPMDSWDILWNP
                     KYKGNIMMFDSVRDTIGITLKKLGYSMNSVNPKEINEAKNLLMKQKDLVLAYVNDEGK
                     DRLLGGEVAMGMLYSGDAVTLMEQNPDLDYAIPKEGTNKWVDAMCVPTTAQNKKEAEL
                     FINFLLDPENAKVNAEYIGYSTPNTGALKLLDPEITQNPVAYPSKEVLDKCETFEDIG
                     ENIKLYDKAWIELKSK"
BASE COUNT         2229 a          600 c         1091 g         1967 t
ORIGIN      
        1 aaaataatga caaaaattgt aactaaattg taataaaatg gtagttattt agcataaaat
       61 aatgtatcat agaagtatat tataaaatta ataaaatagg atttttataa gaaaactgct
      121 ggctgttgat atttaaagtg agtagttaaa ttatgtttag gaggggtaga atttgttaca
      181 aaaagtatta tggaaatttt taaagctagt agtcggttta tttatatgtt cagttggtat
      241 agtattgacg ataaattgta accttggatt atctccctgg gatgtatttc atcaagggct
      301 ttcaaatcat ataggaataa ctataggaac tgcttctata attgtaggct ctatagtagt
      361 tattgctgat gtcgttcttg gagaaaatgt aggatgggga actgttttta acatgttact
      421 aattgggttt tttatggatt tacttttata tagtaattta attccagagg ctgatagttt
      481 atttgtagga ataattatgt tgatattagg attggttctt ttgtctgttg gaatggtatt
      541 ttatatggga agtggtttag gaagtggacc tagagatggt ttgatggtcg cacttcaaaa
      601 gaaaactgga aaatcaacta atttaataag aggtactata gaagttggag cactaatagt
      661 aggattttta ttaggtggaa aagttggtat aggaactata ataagtgcat ttggactggg
      721 gtattttaca caaatggtat ttggcttatt taaattagat tgtagtaaga ttaaacatag
      781 atttatagta gatgatataa aatttatcaa agtgtatatg agtggtgaga aaaaagctgc
      841 tgttcaaaat acagttaagt aatttaagaa taaattcaac gaaatgttaa gtattttaaa
      901 ttaacatttt gtttagatgg ataaattgaa tattaaaaga acattaaaat atgaggattg
      961 ctagaacact ataaaatata gatttctagc aattttttat ttgttcaatg tttttttagt
     1021 attagaagtc ttattctcta tttaatgaat gaattaattt ataattagag atactaaact
     1081 ttatagatat taatataacc acctttaaac tgtgtatttt ccaaagcctt taaaatgctt
     1141 gtatttattc taaataaaaa aataaaaaaa tgtcaaaaaa ctcttgtatt atatccaaat
     1201 tttatatata attacctatg ttgtttagaa attttaaact caatgtttag taaacaaaat
     1261 gtttagtaaa ataaaacaga aactaggagg tgcaacatgg atataggtga aaagataaaa
     1321 agattaagaa cagaaaaaca actgacccag gaagaattag ctaatagatg tgaattgtca
     1381 aagggattta tttctcaatt agaaaataac ttaacatcac catccatagc tacccttata
     1441 gatatacttg aaatactagg tacaaattta agggaatttt ttaatgaaat agatgatgaa
     1501 agaatcagtt ttacaaaaga agatatgttt gaaactgaag atgaagattt aaaatataaa
     1561 ttaaagtggc taattccaaa ttcacaaaaa aatgaaatgg agccaataat aataacattg
     1621 tatccagggg gacagtataa agaagaaaag ccacatgaag gtgaagaatt tggatatgta
     1681 ttagcaggtt caatatatgt ccatataggc gaaaagaaaa ataaagtaaa aaaaggagag
     1741 agtttttact ttagaccgaa agcaaatcac tatatatcaa acgaaggcaa aactacagca
     1801 aaagtgattt gggtaagtac gccaccatca ttttagaata gaggtgttta ataattggta
     1861 gaaaatataa tagaattaag ggatatatct aaacattatg aggaacttac tgttttagat
     1921 aatttcaatt tagatataaa gaaaaatgag tttcttacat tacttggacc aagtggatgt
     1981 ggaaaaacta caacattaaa aataatagca ggatttgaat atgctgatga tggtaaagtt
     2041 ttatttgaag gtaaggagat aaataatttg ccaccatatg aaagacaagt aaacacagta
     2101 tttcaaaaat atgctttatt tccacacatg gatgtttatg aaaatgtagc ttttggacta
     2161 aaaattaaaa agactccaaa agatgtaata gatgctaaag taaaagaggt tttgaaactt
     2221 gttgcactag aaggttttga aagacgacaa gttgaatcat tgagtggagg acaacaacag
     2281 agaattgcaa tagcaagagc tctagtcaat gaaccaaagg tgttgttact agatgaacca
     2341 cttggagcac ttgatttaaa gttaagacaa gagatgcaaa tagaattaaa gagaatccaa
     2401 aaaaaattag gaataacatt tgtttttgta actcatgacc aagaagaggc tcttactatg
     2461 tcagatacta ttgttgtaat gtataagggt aaaattcaac aaatgggaac accacaagat
     2521 atatacaatg aacctaaaaa ttcatttgta gctaaattta taggtgaaag taacatattt
     2581 gatggaatta tgatagaaga ttataaagtt aatttctgta atagagattt tgagtgtgtt
     2641 gataaaggat tcgaaaaaaa cgaaaatata gaagtagtaa taagaccaga agatataaaa
     2701 atggtaaagc cagaagaagg aatgttaaag gggaaagtta catctacagt gtttaaagga
     2761 gtgcattacg agatagaatt gaatgaaaat ggaagattgt ggatacttca taatactaaa
     2821 aatgctgagg ttgggactga actaggcatg gatatatatc cagaagatat ccatataatg
     2881 agaaaagaga gtaactaaga tgaaaagaaa atctttttta gcatatccat atgtagtttg
     2941 gagtgcgata tttgtaataa ttcctttaat actagtggta ttttttagtt ttacaaaaga
     3001 atcaggtgga ggttatgcct ttacacttga gaattataaa gaagtaattg accctatata
     3061 tatgaaagtt tttgggcgtt ctattctttt agcaggagga gctacattaa tatgccttat
     3121 agttggttat ccagtggcat atataatttc aaaggctcgt gtaagtagaa gaggttcatt
     3181 aatattatta tttattcttc ctatgtggat gaactttctt ttaagaacat atgcatgggt
     3241 tgctatatta ggaaaaaatg gacttttaaa tacattttta ggttggtttg gaatacaacc
     3301 attagcaatt ttgtatacta attttgctat acttttaggt atggtgtata actttttacc
     3361 atttatggtt cttccaatat atacagcact atcaaagatg gataatgatt tgataaacgc
     3421 tgctcatgac ttgggtgcaa ataatatgac agtttttagg aaaattatat ttcctttgag
     3481 tttaccaggt gtaatgtctg gaataactat ggtatttatg ccagcagtca ctacttttgc
     3541 aatatcaaga ctgttaggtg gaggtaaaat aatgctggtt ggagatttga ttgaacaaca
     3601 gtttactgtt gttggagatt ggaactttgg ttcagctata tcgatattta tgatgatagt
     3661 aatattaata tctatgtcta taatgtcaaa atttggagat gaatctgaca aagaaggcgg
     3721 tgggttacta ttttaaaatt aaagaaaata acttctaata tatatttagc attagtattc
     3781 ttatttttgt atgcacccat atttgctttg gtactatttt ctttcaatga ttcaaaatca
     3841 atggcaagat ggaatggatt tacatggaag tggtatggtc aattacttca aaatgagagt
     3901 ataatgagtg ctttatatta tactattgta atagctatat tagcgtctgt aatatctaca
     3961 atagtaggga ctataagtgc tataggaata cataagatga gaggtaagag taagaaatta
     4021 atactaaatg taaactatct tcctatttta aatacagaga tagttacagc ggtagctctt
     4081 atgagtctgt ttgtatttgt aaaaatggaa tttggattta caacaatgct attggcacat
     4141 ataatgtttt gtttgccata cgttatactt tcagttttgc caaaaatgaa gcaattacct
     4201 gataatatag aagatgcagc tatggacttg ggagcaacac ctatatacgc tcttagaaaa
     4261 gtaattttac cacaaataaa accaggtata gtttctggat ttttaattgc atttactatg
     4321 tcaatagatg actttataat aagtttcttt aatgctggaa atggtgtaag caatctttct
     4381 atagaaatct atggaatggc tagaagaggt ataaaacctg aaataaatgc attatcaaca
     4441 ataatgtttg cagtggttct aggtttactt ttactagcta ataaaaaaga atctatagta
     4501 agggggacta aataatgatt aagtttaaaa aagtaatatc ccttatagct gtaggtatga
     4561 tgacagttac tttatttaca ggatgtaata aagcaacaga ttctacaaaa gttttaaatg
     4621 tatataatgt tggagattat atagatgagt ctcttataga taagtttgaa aaagaaacag
     4681 gaatagatgt tcaatattca acttatgata ctaatgaaat gatgtatcaa aaagtgaaga
     4741 gtggtagtac acactatgat ttagtatttc cttcagacta tatggtagag aaaatgaaaa
     4801 atgaaggtct tttggaaaaa ttagatttta agaatatacc taatatgaag tatttagata
     4861 agagtttttt aaatcctgta tacgacaaaa ctaatgaata tagtgttgca tatatgtggg
     4921 gaactcttgg aatattgtac aataagaaag aagttaaaga ccctatggat agctgggata
     4981 tactttggaa tccaaagtat aagggaaata taatgatgtt tgactctgtt agagatacta
     5041 taggaataac tcttaaaaaa ttaggatata gcatgaatag tgtaaatcca aaggaaataa
     5101 atgaagctaa aaatctttta atgaaacaaa aggatttagt actggcttat gtaaatgatg
     5161 aaggtaagga tagattgctt ggtggagaag tggcaatggg tatgctgtat tctggagatg
     5221 cagttacact aatggaacaa aatcctgacc ttgattatgc tatacctaaa gagggaacta
     5281 ataaatgggt agatgctatg tgtgtaccta caacagcgca aaataagaaa gaagctgagt
     5341 tgtttataaa ctttttatta gacccagaga atgcaaaagt aaatgctgag tacattgggt
     5401 attcaacacc taatacagga gcactaaaat tattagaccc agaaataact cagaatcctg
     5461 ttgcatatcc atctaaagaa gtacttgata aatgtgaaac ttttgaagat attggtgaaa
     5521 atataaaatt atatgacaag gcttggatag agttaaaatc aaaatagagt cttgttggtt
     5581 taagtaaatt tacttaataa atacttaata aaactgttct tttttagaaa atttattttc
     5641 taattattga atagttttat tttttgttta agttatattt ttgtttaggt catgttttta
     5701 ttttgagttt atttttatga gaatttaaag tccagtaagt ttcattgatt actattttta
     5761 taataataac tataaagagg ctgtctcaaa attgagataa gcttcttttt aattgttgaa
     5821 atgatgagaa catgtttgat ttcatagaaa aaagagtgat tcatgagcta aaaagttcac
     5881 tttgaga
//
LOCUS       DAEPXF010000216         5757 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain HB27
            SAMN13376389-rid12479423.denovo.224, whole genome shotgun sequence.
ACCESSION   DAEPXF010000216 DAEPXF010000000
VERSION     DAEPXF010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13376389
            Sequence Read Archive: SRR10518432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5757)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5757)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001069624.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001069624.1
            Genome Coverage       :: 315x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/15/2021 01:54:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,165
            CDSs (total)                      :: 4,076
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 89
            rRNAs                             :: 10, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 10 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 19 of 69
            Pseudo Genes (incomplete)         :: 44 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 10 of 69
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5757
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13376389-rid12479423.denovo.224"
                     /strain="HB27"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="China: Hebei"
                     /collection_date="2017"
     gene            complement(234..2066)
                     /locus_tag="KRL67_003448"
     CDS             complement(234..2066)
                     /locus_tag="KRL67_003448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBH1524289.1"
                     /translation="MSRMGRGPMGKSMGAGQKANDFKGTMRKLIAYLSKFKISIILVI
                     VFAIGSASFSIVGPKILGKATTKIFEGLVSKVSGGNVGIDFNAIGKILTFLLCLYLIS
                     ALFSFIQGFIMSGISQKVSYNLRKEISAKLDRLPMKYFDTKTHGEILSRITNDIDTLN
                     QSLNQSMTQLITSVTTMIGVLIMMLSISGIMTLVAVLILPISMFVISRIIKKSQKYFR
                     YQQEYLGNVNGQVEETYSGQTIVKAFNREDEVIEEFDKLNDSLYNSAWKSQFLSGIMQ
                     PLMMFIGNLGYVMVSILGGWLAIKKTIEVGDIQSFIQYVRNFTQPMTQIAQVANLLQS
                     TAAASERVFEFLEEEEEDQIVENAVSIDGLEGKIDFENVNFGYNPNKTIINDFSVNVK
                     PGQKVAIVGPTGAGKTTIVKLLMRFYDVNSGSILIDGHNIKDFNRSELREMFGMVLQD
                     TWLFSGSIMENIRYGKLNATDEEVIEAAKSAHVHRFIKTLPDGYKMKLNEEASNVSQG
                     QKQLLTIARAILADPKILILDEATSSVDTRTEVLIQKAMDNLMEGRTSFVIAHRLSTI
                     RDADMILVMNEGDIVEQGNHEELLKKGGFYANLYNSQFEENEAM"
     gene            complement(2059..4305)
                     /locus_tag="KRL67_003449"
     CDS             complement(2059..4305)
                     /locus_tag="KRL67_003449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011009877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBH1524290.1"
                     /translation="MFKLIKYLKKSALSIVIIVCLLVIQAVCDLSLPEYTSNIVNIGI
                     QQGGVENSVPSVIREGELDKITLFMDKSSKDKVLDNYTLLNKKDYVKYKDKYPGLKDE
                     SLYELNTKDKDTIDDLNVIFGKAILIVSGLESNSKEVKAMKAQLMSKLPPQATQSGDV
                     DIFKLLSAMPKEQLDTLTKEMSKGFESMPESMITQSSVSYVRSEYEKIGIDTEKTQNN
                     YILFTGAKMLGIAMISMVATITVGFLAARVAASVGRNLRSGVFRKVMSFSNTEMNEFS
                     TASLITRSTNDIQQIQMLMVMLLRIVFYAPIMAIGGVIKVLNTNTSMAWIIAVAVVAI
                     LSLIVVLFSVVMPKFKLVQKLVDKLNLVSREIITGIPVIRAFSTEKHEEERFDKANKN
                     LTKTNLFVNRVMTCMMPAMMLIMNLITVLIVWRGAYSVDSGAMQVGDMMAFIQYTMQI
                     IMSFLMISMVSIMLPRASVSATRIDEVLVSDISIKDPEKSEAQEFKKDKKGLVEFKNV
                     SFMYPDAEEPILTNISFTAKPGETTAFIGSTGSGKSTLINLIPRFFDVTEGEILLGGV
                     NIKNVSQHDLREKIGYVPQKGVLFSGTIESNLKYGRKDATDEEMRTAAEIAQATEFID
                     SKPEAFKTEISQGGTNVSGGQKQRLSIARAIAKNPEVYIFDDSFSALDLKTDAALRKA
                     LKSKTAESTVLIVAQRISTILNAEQIIVLNEGEIVGIGTHKELLKNCEVYEQIALSQL
                     SKEELANE"
     gene            complement(4373..4834)
                     /locus_tag="KRL67_003450"
     CDS             complement(4373..4834)
                     /locus_tag="KRL67_003450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HBH1524291.1"
                     /translation="MNSNSEEKLNDVFLQLMRIYFIRTHFYFDKLGLYHGQPQILFSL
                     KMKNGQSQRELADKRKVKASTTTVMIKRMEKAGLVERKQDEKDQRVSRIFLTDKGLEI
                     CNKVEEFNKELEREFFLGFSEEEKIVLRRLLMQVRDNLKAGCHKHEDEKYF"
     gene            complement(5138..5704)
                     /locus_tag="KRL67_003451"
     CDS             complement(5138..5704)
                     /locus_tag="KRL67_003451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase"
                     /protein_id="HBH1524292.1"
                     /translation="MRKLEEILEYNKSFVENKEYEQYVTSKHPNKKIVVLSCMDTRLT
                     ELLPKAMNLKNGDVKLIKNAGATIMHPFGSIMRSVLVAIYEFDVDEVMVVGHHGCGMC
                     NVDTDKLLGKILDRGISNDVILTLRSAGIDTKQWLHGFDSAEESVKDSIDLIKGHPLI
                     PDGIIVHGLIISPETGKLDVVVNGYEDK"
BASE COUNT         1829 a         1085 c          623 g         2220 t
ORIGIN      
        1 tcacctccaa cattttcctt actacatatt ataataaaat atacactttg taaagacata
       61 aaattatgtg gctatctcaa aatatagaga atcttctttt tcattgttaa aatgataaaa
      121 atacatttta tttcatacac aaaagagtgc cttatgaact aaaaagttca ttttaagaca
      181 ccctcattta ttttatctta ttaattgtat gtctatgcta agatagttgt ttgttacatt
      241 gcttcatttt cttcaaattg agaattgtaa agattagcat agaatccgcc tttctttaga
      301 agttcctcat gatttccttg ttcaactata tcgccttcat tcattacaag aatcatatca
      361 gcatcacgta tagttgataa tctgtgggct ataacaaaac ttgttctacc ttccataaga
      421 ttatccatag ctttttgaat caatacttct gttctagtat cgacagaact tgtagcttca
      481 tctaatatta aaattttagg atcagctaaa atagcacgag ctatagtcaa taattgtttt
      541 tgaccttgag aaacattact tgcttcttca tttaatttca tcttatatcc atctggtaat
      601 gtcttaataa atctatgaac atgtgcagat ttagcagcct ctataacttc ttcatctgta
      661 gcattcaact taccatatct tatattttcc ataattgaac cactaaataa ccatgtatct
      721 tgaaggacca taccaaacat ttctctaagt tcactacgat taaaatcttt aatattatga
      781 ccatcaatca gtattgaacc gctattaaca tcataaaatc tcattaaaag tttaactatt
      841 gtagttttac ctgctcctgt aggtccaaca atagcaactt tttgaccagg tttaacattt
      901 actgagaaat catttattat agtcttgttt gggttgtatc caaagttgac attttcaaaa
      961 tcaattttac cttctaaacc atctattgaa actgcatttt caacaatttg gtcttcttct
     1021 tcctcttcta aaaactcaaa tactctttca gaagctgctg ctgttgattg caataaattg
     1081 gcaacctgtg caatctgagt cattggctgt gtaaagtttc ttacatattg tataaatgac
     1141 tgtatgtcac caacctcaat tgttttctta attgcaagcc aacctcctaa gattgacacc
     1201 attacatatc ccaagtttcc aataaacatc ataagtggtt gcattatacc tgataaaaat
     1261 tgagacttcc atgctgaatt atacaaacta tcatttaact tatcaaactc ttcaattact
     1321 tcatcttcac gattaaatgc tttcactatt gtttgaccac tgtaagtttc ttcaacttga
     1381 ccatttacat ttcctaaata ttcttgttga tatctaaagt atttttgtga tttctttatt
     1441 attcttgaaa ttacaaacat tgatattggt aatattaaaa ctgccactaa tgtcataatt
     1501 ccacttatag ataacatcat tatcaaaaca cctatcatag ttgttactga tgttataagc
     1561 tgtgtcatac tttgatttaa actttgattt aaagtatcta tatcattcgt aattctcgat
     1621 aatatttcac catgcgtctt agtatcaaaa tacttcattg gcaatctatc caattttgct
     1681 gaaatctctt ttcttaaatt ataggatact ttttgagaaa ttccactcat tataaatccc
     1741 tgtataaaag agaacagtgc acttatcaaa taaagacaaa gtaaaaaggt taaaattttt
     1801 ccaattgcat taaaatctat acctacattc cctccagaaa ctttactcac taacccttca
     1861 aatatctttg tagttgcctt tcctaaaatt tttggaccta ctatagaaaa cgatgcacta
     1921 ccaatagcaa atacaattac tagaattatt gatattttaa atttagataa atatgcaatt
     1981 agttttcgca tagtcccttt aaagtcattt gctttttgtc ctgctcccat agattttccc
     2041 ataggacctc ttcccattct actcattagc tagctcctcc tttgaaagtt gtgataaagc
     2101 tatttgctca taaacttcgc aattttttag aagctcttta tgtgttccta ttccaactat
     2161 ttctccctca tttaaaacaa ttatctgttc tgcatttaag atagtgctaa ttctttgtgc
     2221 aactattaga actgtacttt cagcagtttt tgattttaaa gccttacgaa gagctgcatc
     2281 agtttttaag tcaagtgctg aaaaactatc atcaaatata taaacttctg gattttttgc
     2341 tattgctctt gcaatagaaa gcctttgttt ttgacctcca gatacatttg ttccaccttg
     2401 agaaatttca gttttaaaag cttccggttt agaatctata aactctgtag cttgagctat
     2461 ttcagctgct gttctcattt cttcatctgt tgcgtctttt ctaccatatt ttaagttgga
     2521 ttctatagta ccagaaaaca atactccctt ttgaggtaca tatccaattt tttcccttaa
     2581 atcatgttga gaaacatttt ttatattaac tccaccaagt aatatttctc cttcagtaac
     2641 atcaaagaaa cgaggaatta aatttataag agttgactta ccactaccag tacttcctat
     2701 aaaggcagtt gtttctcctg gttttgctgt aaaagatata tttgtcaata taggctcttc
     2761 tgcatctgga tacatgaatg aaacattttt aaattcaaca agaccctttt tatctttctt
     2821 aaactcttgt gcctctgatt tttctgggtc ttttatgctt atatctgaca ctaatacttc
     2881 atctatacgt gtagctgaaa cggatgctct tggaagcatg atagatacca ttgatatcat
     2941 taagaatgac attattattt gcattgtata ttgtataaat gccatcatat ctccaacttg
     3001 cattgctcca gaatcaacac tatatgcacc tctccaaact ataagaactg tgataaggtt
     3061 cattataagc atcatagcag gcatcataca tgtcataact ctatttacaa aaagatttgt
     3121 tttagttaaa tttttatttg ctttatcaaa tctttcttct tcatgttttt cagtactaaa
     3181 agctctgata actggaatac cagtaattat ttcacgactt actaagttta acttatcaac
     3241 aagcttttga actagtttaa acttaggcat aaccactgag aataacacta caattaatga
     3301 taaaatagca actactgcta ctgctataat ccaggccatt gaagtatttg tgtttaaaac
     3361 ctttatcact ccacctatag ccattatcgg tgcatagaat actattctaa gcaacataac
     3421 catcaacatt tgtatctgtt gaatatcatt tgtactacga gtaattaatg acgctgtaga
     3481 aaattcattc atttcagtat ttgaaaatga cataactttt ctaaagactc cacttcttaa
     3541 atttctacct acacttgctg ctactcttgc tgctaaaaat cctactgtta ttgtagctac
     3601 catactaatc attgctatac caagcatttt agcaccagta aataatatat aattattttg
     3661 agttttttct gtatcaatac ctattttctc atattctgac cttacatatg aaaccgagct
     3721 ttgtgttatc atactctctg gcatactttc aaagcctttg ctcatctctt tagtcaatgt
     3781 atctaactgt tcctttggca ttgctgatag caatttaaat atatcaacat ctccactttg
     3841 agtagcttgt ggtggaagtt tactcattaa ctgagctttc atagctttta cctctttact
     3901 attgctctca agccctgata ctattaaaat agctttacca aaaataacat ttaaatcatc
     3961 tatagtgtct ttgtctttag tatttaactc ataaagagat tcatctttta gacctgggta
     4021 cttatcttta tacttaacat aatccttttt attcaacaaa gtataattat ctagtacttt
     4081 atccttacta cttttatcca taaataaagt gattttatct aattctccct ctctaatcac
     4141 agatggaaca gagttttcta ctccaccttg ttgtatacct atatttacta tattggatgt
     4201 gtattctggt aaagaaaggt cacataccgc ttgaattacc aatagacaga ctattattac
     4261 tattgataat gccgatttct ttaaatattt aattaattta aacatacatt tcttcccttc
     4321 tgtaatatta attagtctaa taacattaaa ttgaaaaaca ttattttaaa ccttaaaaat
     4381 atttctcatc ttcatgctta tgacatcctg cttttaaatt atctcgtact tgcattagaa
     4441 gtcttctaag tactattttt tcttcttcac taaatcctaa aaaaaactct ctttccaatt
     4501 ccttgttaaa ttcttcaact ttattacata tttctaaacc tttgtctgtc aaaaaaatcc
     4561 tagatactct ttggtccttt tcatcttgtt ttctttccac aagtccagct ttttccatcc
     4621 tctttatcat aactgtagtt gtagatgctt ttactttcct tttatctgct aattcccttt
     4681 gactttgccc atttttcatc tttaaagaaa aaagtatttg tggttgacca tgatataagc
     4741 ctaatttatc aaaataaaaa tgagttctta taaaatatat tcgcattaat tgcaagaaaa
     4801 catcatttaa tttttcttca gaattgctat tcatacttac cctccttatt tttatattac
     4861 ttagtcggct aagcattatt ttactacacc atatacattt tttcaatata tttataaaaa
     4921 attcatataa atttcattta ttaaatattt tcacatcata tagttataat tttaataaat
     4981 aatagtaaga tttttaatta gtcagctact tatatttttt attgtaaagc ttaaaataaa
     5041 ccttttcata tattttaaaa taaaaaagtt gtcccaaaat aaattttgaa acaacatctt
     5101 acttgacagt atcttgatta tataacatta taatactcta tttatcttcg tatccattta
     5161 caacaacatc taacttccca gtttctggtg atataattag accatgtaca ataataccat
     5221 ctggaattaa aggatgccct tttattaaat caatactatc ttttacagat tcttctgcag
     5281 aatcgaatcc atgcaaccat tgttttgtat caattccagc actacgaagt gtaagtatta
     5341 catcattaga tattcctcta tctaaaattt taccaagtaa cttatccgta tcaacattgc
     5401 acataccaca tccatgatgc cctacaacca taacctcatc tacatcaaat tcgtaaattg
     5461 caactaaaac acttctcata atacttccaa aaggatgcat tatagtagct cctgcatttt
     5521 ttattagttt cacatctcca tttttcaaat tcatagcctt aggcaaaagc tcagttaatc
     5581 tggtatccat acaagataag actactattt ttttatttgg atgttttgat gtcacatatt
     5641 gttcatattc tttgttctct acaaatgatt tattatactc taatatctct tcaagttttc
     5701 tcatatgtaa tatcaccctc tttaaaaatt tttttattat tatatcacta ttaaagt
//
LOCUS       DAEPXF010000217         5737 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain HB27
            SAMN13376389-rid12479423.denovo.225, whole genome shotgun sequence.
ACCESSION   DAEPXF010000217 DAEPXF010000000
VERSION     DAEPXF010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13376389
            Sequence Read Archive: SRR10518432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5737)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5737)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001069624.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001069624.1
            Genome Coverage       :: 315x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/15/2021 01:54:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,165
            CDSs (total)                      :: 4,076
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 89
            rRNAs                             :: 10, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 10 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 19 of 69
            Pseudo Genes (incomplete)         :: 44 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 10 of 69
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5737
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13376389-rid12479423.denovo.225"
                     /strain="HB27"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="China: Hebei"
                     /collection_date="2017"
     gene            complement(<1..885)
                     /locus_tag="KRL67_003452"
     CDS             complement(<1..885)
                     /locus_tag="KRL67_003452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HBH1524293.1"
                     /translation="MYNKIKYIPINCEIACNKLKRSMPYKWDLNIYRGCEHGCKYCYA
                     IYSHKYINSNNYFEEIYVKTNIVEKLERQLKSSKWNREVINIGGVTDSYQPIEADYKI
                     MPEILNLLIKYKTPAIISTKSDLILRDYDLIDKLSRITYINVASTITTVNEDTQRLIE
                     PNGVDSMRRFKMLKEFRKTNASVGLHIMPIIPYITDDFDNINSLFRHAKECNVHYVLP
                     GTLYLRGITRGVFFDFVKKEFPELFDKLSILYSTGSLNREYKNQLYKMINELRYKYSL
                     SSSYSKIMKEKLKNSGDVQ"
     gene            complement(935..1396)
                     /locus_tag="KRL67_003453"
     CDS             complement(935..1396)
                     /locus_tag="KRL67_003453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine dimer DNA glycosylase/endonuclease V"
                     /protein_id="HBH1524294.1"
                     /translation="MRLWHKDLIDVLPKNQLVSQWRELLAIKGSIDKKGTPNHLLVNK
                     VLNYSIDEFKFYTKIVHDEMLKRNYKPNELKYTSILKWKNRNFANDISNEHSLNLENL
                     YDDWHNKMYLKQCLYNLEEKATCGGIPINEWNILLCKYSEDYELWSGNIMF"
     gene            complement(1448..1903)
                     /gene="def"
                     /locus_tag="KRL67_003454"
     CDS             complement(1448..1903)
                     /gene="def"
                     /locus_tag="KRL67_003454"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HBH1524295.1"
                     /translation="MAIRKIRTFDDEILRKKSKYVENVDNKIREILNDMAETMYNTPN
                     GGGLAACQVGVLKRLVVIDLGEGLIKLVNPEIIKQEGEQIVVEGCLSFPEVWGKLKRP
                     KKVTVQALNEYGEKIEIKGSGFMAKCLCHEIDHLNGIVFTDKIIEHVKL"
     gene            complement(1875..2138)
                     /locus_tag="KRL67_003455"
     CDS             complement(1875..2138)
                     /locus_tag="KRL67_003455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBH1524296.1"
                     /translation="MKNKIKILREKLGLTQEQLGRLVGTSRQAINAIETGKNEPSIWL
                     AYDISRIFNEPIESVFLFEESERKSRAQISRGEYYGYKKDKNI"
     gene            complement(2235..2951)
                     /locus_tag="KRL67_003456"
     CDS             complement(2235..2951)
                     /locus_tag="KRL67_003456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBH1524297.1"
                     /translation="MIKIINAFKQEFEQIKRDAMLFIVCVSPILCGVFIKFGIPLIQN
                     ISLNKFYYQLNLEPYFLMFDLLLAFITPFMFFFASTMVILGEIDDSISKYLIVTPLGK
                     TGYLVSRFGIPGILAFIITMILLIFFSLTRISFLLNLSISLLVLLQGVIISILVISLS
                     SNKLEGMVITKFSGVFMMGILAPFFILNKVQYILFFLPTFWISKAFKEDKYIYMFISI
                     VISLIWILLLFKKFSKKIVK"
     gene            complement(2948..3631)
                     /locus_tag="KRL67_003457"
     CDS             complement(2948..3631)
                     /locus_tag="KRL67_003457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBH1524298.1"
                     /translation="MRLKNLILGDMKFQFKYGFYFLYLFLSIIYICIINVFPTFMREK
                     IAIIMIYSDPAAMGLFFMGAIVLLEKSQRVLNSLAVSPVKVSEYILSKVISLGVISSI
                     VAMFIAITLNLDNIIISTIGTFFSSIIFSLLGLILASKASSLNQFIVLSIPIEIICFI
                     PPILNVLVDTKSYANLYPFNICISLISGDKNFIMINILILISIIIIIYFITYYFICNS
                     WKKVGGVKL"
     gene            complement(3628..4473)
                     /locus_tag="KRL67_003458"
     CDS             complement(3628..4473)
                     /locus_tag="KRL67_003458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBH1524299.1"
                     /translation="MITVDDLSFSYTDRDFLQNINFEVGKGEILGFLGPSGAGKSTLQ
                     KILIGMITNYGGSVIVNGVESKRHSNKFYENIGVDFEFPSLYEKLTAIENLKYFGSLY
                     SKKLLSIDELLKSVGLENEANKRVSEYSKGMKSRLNFIKALLHNPDILFLDEPTSGLD
                     PSNSKVMKDIILSEKSKGKTIILTTHNMLDATELCDRVAFIVNGKISALDTPHNLIMS
                     KGAIKVRYTYFDNGEKTSECFLNNTANDKNLNMLIEKNKLLSIHSSEPTLNDIFIEIT
                     GRNLQ"
     gene            complement(4587..5177)
                     /locus_tag="KRL67_003459"
     CDS             complement(4587..5177)
                     /locus_tag="KRL67_003459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBH1524300.1"
                     /translation="MPKSYSDKEREYIIKRLKEEARLCMEQYGIRKTTVDELVKRVKI
                     PKGTFYLFFQSKELLFFEVLRDIHDSIQKEILYEINRVDESITCEQLTDIFMKFYRMV
                     DSTSILNLMINGEFEILVRKLPDSIIEEHFRHDDFEIGEIISCIPNAKNKDIESFSGA
                     FRAVFLTMLYKREVGSNCFEDALRLMINGLVIQLME"
     gene            5466..5699
                     /locus_tag="KRL67_003460"
     CDS             5466..5699
                     /locus_tag="KRL67_003460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HBH1524301.1"
                     /translation="MATTTKNRFYDYVQENLNKYVEVHSYFSSCPLYGEIIEADSLSI
                     TLCSRYIDESNPTLNETYTLYLPLSSIISIRTL"
BASE COUNT         2024 a          905 c          556 g         2252 t
ORIGIN      
        1 ctgtacatct cctgagtttt ttaacttttc cttcattatt ttagagtaac tgcttgatag
       61 agaatattta tatctaagtt cattaatcat tttatacaat tgatttttat actccctatt
      121 aagtgaacct gtgctatata gtattgataa tttatcaaat aattcaggaa actctttttt
      181 aacaaagtca aaaaaaacac ctctagttat accccttaag tataaagtcc caggtaaaac
      241 ataatgtaca ttacattctt ttgcatgtct aaatagtgaa tttatattgt caaaatcatc
      301 tgtaatatat ggtataattg gcataatatg tagaccaact gatgcgttag tttttctaaa
      361 ttcttttaac attttaaatc gtctcattga atccacacca tttggctcaa taagcctttg
      421 tgtatcttca tttactgtgg taattgtaga tgctacattg atatatgtaa tccgagatag
      481 cttatcaatt aaatcgtaat ctcttaaaat taaatctgac tttgtagaaa taatagctgg
      541 tgtcttgtac tttatcagca gatttaatat ttctggcata attttatagt cagcttctat
      601 tggttgataa ctatctgtaa ctcctcctat attgattact tctctattcc atttactgct
      661 tttaagttgt ctctcaagtt tttctactat atttgttttt acataaatct cttcaaaata
      721 attattggag ttgatgtact tatgagagta aatagcataa cagtatttac atccatgctc
      781 acatccacga taaatattta aatcccattt atatggcata ctccttttta atttattaca
      841 agctatttca caatttattg gaatatattt tattttatta tacaaataat cacctcttta
      901 tatataataa tattataatg ttaatctata gtaattaaaa catgatattt ccagaccaca
      961 attcataatc ctcactatat ttacaaagta atatattcca ttcatttatt ggtattcctc
     1021 cacatgttgc tttttcttct aaattatata agcattgctt taaatacatt ttattatgcc
     1081 aatcatcata taaattttct aaatttaaac tatgttcatt agatatatca tttgcaaaat
     1141 ttctattttt ccactttaat atacttgtat atttaagttc attaggttta taatttcttt
     1201 ttaacatttc atcatgtaca atttttgtat aaaatttaaa ttcatctata ctataattca
     1261 atactttatt cactaataga tggtttggag tacctttttt atctatagaa ccttttatag
     1321 caagtagctc tctccattga cttactaatt gatttttagg taatacatca attaaatcct
     1381 tatgccataa tctcataatt taactcctta ctataaaata ttttataaat tcaataacca
     1441 atctatctta caattttaca tgttcaataa ttttatctgt aaaaactata ccatttaaat
     1501 ggtctatctc atgacaaaga cattttgcca taaaacctga acctttaatt tctattttct
     1561 caccgtattc gtttagagct tgcactgtaa ctttttttgg tctttttaat ttaccccata
     1621 cttctggaaa acttaaacat ccttcaacaa caatttgttc cccttcttgt ttaataattt
     1681 caggattaac aagcttaata agtccttctc ctaaatctat aacaactagt cgctttaata
     1741 ctcctacttg gcatgcagct aatccgcctc catttggagt attgtacatg gtctctgcca
     1801 tgtcatttaa tatttctctt atcttattat ctacgttttc tacgtactta ctcttttttc
     1861 ttaaaatttc atcatcaaat gttcttatct ttcttatagc cataatattc tcccctactt
     1921 atctgtgctc ttgattttct ttcactttct tcaaataaaa atacactttc aattggttca
     1981 ttaaaaattc ttgaaatatc atatgccaac catattgaag gttcattttt accagtttct
     2041 atagcattta tagcctgtct agatgttcca actaatcttc ctagttgctc ttgtgtcaat
     2101 cctaattttt ctcttaatat ctttatttta tttttcatat ttgctccatt catgtaaggt
     2161 taactttaca ttttaaatat aacatttttc aacattgctg tcaagtcaac cttacattta
     2221 tatagtaaat tttcttattt aactattttt ttactaaact ttttaaataa taataaaatc
     2281 catattaatg atataacaat tgaaataaac atatagatat atttatcttc cttaaatgcc
     2341 ttcgatatcc aaaaagttgg taagaagaac agaatatatt gcactttatt taaaataaaa
     2401 aatggtgcta aaatacccat cataaaaaca cctgagaatt tcgtaattac cattccttct
     2461 agtttatttg atgataaaga aatcactaat attgatataa taactccttg taataaaact
     2521 aataaagaaa ttgataaatt taacaagaaa gaaattctag ttagagaaaa aaagataagt
     2581 agaatcatag taataataaa tgctaagata cctggtattc caaatcgtga aacaagataa
     2641 ccagttttgc caagcggagt tactattaaa tattttgaaa tactgtcatc aatttctccc
     2701 aatatgacca ttgtagaagc aaaaaagaac ataaaaggtg taataaaagc taaaagtaaa
     2761 tcaaacatca agaaatacgg ctctaaattt aattgataat aaaacttgtt aagtgatata
     2821 ttctgtatta atggtattcc aaattttata aatactccac ataatattgg agaaacacat
     2881 actataaaaa gcatagcatc tctcttaatt tgttcaaact cttgtttaaa cgcatttata
     2941 atctttatca tagttttact ccacctacct ttttccaact gttacaaata aaataataag
     3001 ttataaaata aattattatt ataattgata ttaaaattaa aatattaatc attataaaat
     3061 ttttatctcc actaataaga gaaatacata tattaaaagg atacaaattt gcatatgatt
     3121 ttgtatcaac aagtacattg agtattggtg gaataaagca tataatctca ataggaatac
     3181 ttaaaacaat aaactgattc aaactagatg ctttacttgc aagtatcaat ccaagcaaag
     3241 aaaatataat agaagaaaaa aaagtaccta tagttgatat tataatatta tctaaattta
     3301 atgtaatagc aataaacata gctacaatac tagatattac tcctaatgaa attacctttg
     3361 ataaaatata ctcagatact tttactggag atacagctaa ggaatttaaa accctttgac
     3421 ttttctcaag aagtacaata gctcccataa aaaacaatcc cattgctgct gggtcagaat
     3481 aaatcataat tatagctatt ttttctctca taaaagttgg aaatacattg ataatacaaa
     3541 tatatataat acttaaaaaa agatataaaa aataaaagcc atacttaaat tgaaacttca
     3601 tatctcctaa aataagattt tttagcctca ttgtaaattc ctccctgtta tctcaataaa
     3661 aatatcattt aatgttggtt cactactatg tattgataaa agtttatttt tctcaataag
     3721 catattcaaa tttttatcat tagcagtatt attcagaaaa cattcagaag ttttttctcc
     3781 attatcaaag taagtatatc taactttaat agctcctttt gacataataa gattatgtgg
     3841 tgtatctaaa gcagatattt taccatttac aatgaacgct actctgtcac aaagctctgt
     3901 tgcatctaac atgttatgag ttgttaaaat aatagtttta cctttagatt tttctgataa
     3961 aattatatct ttcataacct tgctatttga tgggtcaaga ccacttgttg gttcatctaa
     4021 aaataaaata tctggattat gtaataaagc ttttataaaa tttaatctag atttcatacc
     4081 ttttgaatac tcagataccc ttttatttgc ttcattttcc agtcctactg attttaataa
     4141 ctcatcaata gatagtaatt ttttagaata tagagaccca aaatatttta aattttctat
     4201 tgcagtcaat ttttcatata aactaggaaa ttcaaaatca acgcctatat tttcataaaa
     4261 tttatttgag tgtcttttac tctctactcc atttacaatg acacttcctc cataattagt
     4321 tatcattcca attagaatct tttgtaatgt agattttcct gcacctgatg gtcctaaaaa
     4381 tcctagtatt tccccttttc ctacttcaaa gttaatattt tgtaaaaaat ctctatccgt
     4441 ataactaaat gataaatcat caactgtaat cattcaatat caccctttca actttttgac
     4501 tattttgact atatggtcaa taaaatataa tttttttgtt tgttaatact ttttaataaa
     4561 agttattagc aaacaaaatt taaattctat tccataagtt gtataactaa tccgtttatc
     4621 attaatctta aagcatcttc aaaacaatta cttccaacct cgcgcttata taacatggta
     4681 agaaaaactg ctctaaatgc tccactaaag ctttctatgt ctttattttt tgcatttggt
     4741 atgcaagata taatctctcc aatttcaaag tcatcatgtc taaagtgttc ttcaattata
     4801 gaatctggta actttctcac caatatctca aattctccat ttatcatgag atttaaaatt
     4861 gaagtactat caaccatcct atagaatttc ataaatatat ctgtcaattg ttcacatgta
     4921 atactttcat ctactctatt tatctcatat aagatttctt tttgtataga gtcatgtata
     4981 tctctcaata cctcaaaaaa gagcaattct tttgattgaa aaaatagata aaaagttcct
     5041 tttggtattt ttacccgttt tacaagctca tcaacagtag tttttctaat accatattgc
     5101 tccatacaca atcttgcttc ttctttaagt ctttttatta tatactctct ttctttatca
     5161 gaatacgatt taggcatctt tttcctccaa tttgactaaa ataatttata tagtcaatat
     5221 aatacttttt ctatctaatg tcaatagtat tattagtata tttaaaaata agcttaaaaa
     5281 ctttcgaaaa ttctgaattt tatatagcat tttttattat ctagtgttat aattaagata
     5341 taaataatat attattctaa ttttcatatc tatttgctta tttcttaact tagtttctaa
     5401 tagaagtaat catatttatc tttttaaaag tttcaacttt aataaaaaat aaaggagttg
     5461 attttatggc tactactaca aaaaaccgtt tttacgatta tgttcaagaa aacttaaata
     5521 aatatgttga agtacattct tactttagca gttgtcctct atatggtgaa attatagaag
     5581 cagatagctt atcaattaca ctttgctcaa gatatataga tgaatctaat ccaactttaa
     5641 atgaaactta cacattatat ttaccattaa gttcaataat atctattaga acactataaa
     5701 aagttaatta aatatgggtt atttatatat aaaatgt
//
LOCUS       DAEPXF010000218         5711 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain HB27
            SAMN13376389-rid12479423.denovo.226, whole genome shotgun sequence.
ACCESSION   DAEPXF010000218 DAEPXF010000000
VERSION     DAEPXF010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13376389
            Sequence Read Archive: SRR10518432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5711)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5711)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001069624.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001069624.1
            Genome Coverage       :: 315x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/15/2021 01:54:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,165
            CDSs (total)                      :: 4,076
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 89
            rRNAs                             :: 10, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 10 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 19 of 69
            Pseudo Genes (incomplete)         :: 44 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 10 of 69
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5711
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13376389-rid12479423.denovo.226"
                     /strain="HB27"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="China: Hebei"
                     /collection_date="2017"
     gene            <1..1435
                     /locus_tag="KRL67_003461"
     CDS             <1..1435
                     /locus_tag="KRL67_003461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HBH1524302.1"
                     /translation="LILILLTLFSINVFILYLKIKLYEDKINLGLINNSDKFINITKD
                     ENFLKHVQNYIISNEKENCVMACFDLCRLKLINDIYGYELGDEVLNNILSNLKNYFGK
                     EAIYGKLKSDVFVLIIKLENREQKIPYLVNLIKNKIVILSQIDSFKMDINIEASIGIY
                     KFNNNEIDIKKAIDNADMARLKSKGLKHIEYVEFDNAMEEEIQSIMKIERDLFLAIKN
                     KEFVIHYQPKVDSSTGNIIGSEALIRWLHPALGMVGPNKFIPIAEKNGLINNIGRWLI
                     QEVFITINKWISEGINVLPVSINISRVELYKNDLVDFFKLMFNTYNIPKELIELEITE
                     TTALRDVRFISERLYEIKSLGMKISLDDFGVGNSNFINLKGIPLDIIKIDRSLILDIV
                     TNTKTKFMVKAIVNLSHDLNVTVICEGVEDMHQVKVLSELGCNFIQGYVFFKPLDETN
                     YKKLLTDGSYINLEDTLLDKCMAVEEE"
     gene            1438..1836
                     /locus_tag="KRL67_003462"
     CDS             1438..1836
                     /locus_tag="KRL67_003462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HBH1524303.1"
                     /translation="MISEDVDYSIRIIRAIFNNGEDKPIDAKMILKSEDIPILTLYRL
                     LRKLCANNILTFNKEDGKTYMLNVSLDTISLRTIVEVIDGKIYINKCTRYVSNCSNCL
                     KGCKVNRELLEIEKEIYKLLSRKSIRQILE"
     gene            1875..3608
                     /locus_tag="KRL67_003463"
     CDS             1875..3608
                     /locus_tag="KRL67_003463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HBH1524304.1"
                     /translation="MDDISQDNFLLSKEYENSLDVDTKKASGIYYTPKIIVDYIVKKT
                     LKNHDIIKNPYPRILDISCGCGNFLLEVYDILYDLFEENIYELKKKYDENYWIVDNIH
                     RHILNYCIYGADIDEKAISILKDSLTNKKVVNDLDESDIKINLFCCDSLKKKWRYKFD
                     YIVGNPPYIGHKKLEKKYKKFLLEKYSEVYKDKADLYFCFYKKIIDILKQGGIGSVIT
                     PRYFLESLSGKDLREYIKSNVNVQEIVDFLGANIFKNIGVSSCILTFDKKKTKETYID
                     VFKIKNEDICINKFETLEELLKSSKFEHFNINQRLLSDEWILVNKDDETFYNKIQEKC
                     KYSLEDIAISFQGIITGCDKAFILSKDDVKLNLVDDKFLKCWIKSKNINKYIVDKSEY
                     RLIYSNDIDNENTNKRILDEIIGVYKTKLENRRECKSGIRKWYELQWGREKLFFERKK
                     IMYPYKSNENRFAIDYDNNFSSADVYSFFIKEEYLDKFSYEYLVGILNSSVYDKYFKI
                     TAKKMSKNIYDYYPNKVMKIRIFRDNNYEEIENLSKQIISILLNKSIDKGKVEKLQIK
                     MDNLIMDSLGI"
     gene            3840..5051
                     /locus_tag="KRL67_003464"
     CDS             3840..5051
                     /locus_tag="KRL67_003464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HBH1524305.1"
                     /translation="MKGLVLEGGGTKGAYQIGAYKALRELGIEFQGVTGTSIGALNGA
                     YIIQNDIEVMEDIWLNYDYTHFMNIDRETYERFKNVDFTTKNLNAVFELINKARKNEG
                     IDISPLRQLMETTLNEDIIRKSKKDFGLVTVWWDKKINPYPLYIEDIPKGRLIDYLIA
                     SSSLPIFQTNMMDDKLYLDGMFCDNMPIGLLEDKGYEDVVVIRLIDDFIGKMNLNRHQ
                     NINIKTIIPSEYLGGSLNKDRDNVEKNIRLGYLDTMKAYERCTGIKYYFDVEYKYNEE
                     YCFDKFRSMSNETIQYICNLLNIKRDASLRTVMEIVIPKLGEVLGLPKDFSYKDLFYC
                     IYEKKLEENNINRIKLYDFNKVIDVVNTNIDSNRNLASNFEVRPKISIKKNKNTKLLT
                     NCIIKDIKNQS"
     gene            5243..5503
                     /locus_tag="KRL67_003465"
     CDS             5243..5503
                     /locus_tag="KRL67_003465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HBH1524306.1"
                     /translation="MEKVVSIKNASGLHARPAGMFVKKAAEFKSTVEVVAKDKTVNAK
                     SIMGIMSLGLAQGEELKLVVNGEDEEVALAAMVELVESGFGE"
BASE COUNT         2409 a          477 c          963 g         1862 t
ORIGIN      
        1 tttaatttta attttattaa ccttatttag tataaatgta tttattttat atcttaaaat
       61 taagttgtat gaggataaaa ttaatttagg attgataaat aattcagata agtttataaa
      121 tattactaaa gatgagaact tcctaaaaca tgttcaaaat tatattatca gtaatgaaaa
      181 agaaaactgt gtaatggctt gttttgattt atgtagactc aagcttataa atgatatata
      241 tggttatgag ttaggggatg aagtgttaaa taatatttta agtaacttaa aaaattattt
      301 tggaaaagaa gctatttatg gaaagttaaa aagtgatgtt tttgtattaa taataaagct
      361 tgaaaatagg gaacagaaga ttccttattt agtaaatctt attaagaata aaattgtgat
      421 actaagtcag attgacagtt ttaaaatgga tataaatatt gaggcttcta ttggaattta
      481 taaatttaat aataatgaaa ttgatataaa aaaagctata gataatgctg atatggcaag
      541 gcttaaatca aaaggactaa aacacataga gtatgtagaa tttgataatg ctatggaaga
      601 agaaatacaa tcaattatga aaatagagcg agatttattc ttggctatta aaaataaaga
      661 gtttgtaatt cactatcaac ctaaagttga ttcaagtaca ggtaacatta taggttcaga
      721 ggcattaata agatggttgc accccgcatt aggcatggtt ggaccaaata agtttatacc
      781 tatagctgaa aaaaatggtc tgataaacaa tattggtaga tggctaattc aagaagtttt
      841 tattaccatt aacaagtgga tttcagaagg tataaatgtc ttaccagtct caataaatat
      901 ttctagagta gaactatata aaaatgattt agttgatttt tttaaactga tgtttaatac
      961 atataatatt ccaaaagaac ttatagaatt agaaattaca gaaacgacag cactaagaga
     1021 tgtaagattt ataagtgaaa ggctttatga gattaaatct ttaggaatga agatatcatt
     1081 agatgatttt ggagtaggta attcaaattt tatcaatctt aaggggatac cactagatat
     1141 tataaaaatt gataggtcac ttatattgga tatagttaca aatacaaaaa caaagtttat
     1201 ggtgaaagca attgttaacc tttcacatga tttaaacgta actgttatat gtgaaggggt
     1261 agaggatatg catcaggtca aagttttaag tgaactggga tgtaatttca tacaaggtta
     1321 tgtatttttt aaacctttag atgagacgaa ttataaaaaa cttcttactg atggaagtta
     1381 tataaatctg gaagatactc ttttagataa atgcatggca gttgaggagg agtaaggatg
     1441 atttcagaag atgtagatta ttcaataaga atcataagag ctatatttaa taatggagaa
     1501 gataaaccaa tagatgctaa aatgatatta aaaagtgaag atataccaat actaacacta
     1561 tacagactgc ttagaaagct ttgtgcaaac aatattctta cttttaacaa agaagatggc
     1621 aagacataca tgttaaatgt gtcattggat acaataagtc taagaactat tgtagaagta
     1681 attgatggaa aaatatatat aaataaatgt acgagatatg tatctaattg cagtaattgc
     1741 ttaaaaggtt gtaaagtaaa tcgtgaattg ctagaaatag aaaaagaaat ttataaactt
     1801 ttatcgagaa aatctataag acaaatacta gaataaaaat tttcagctat gataataatt
     1861 ggaggttttc ggttatggat gatatatctc aagataattt tctactatct aaagagtatg
     1921 agaatagctt agatgtagat actaaaaaag caagtggaat atattatact cctaaaataa
     1981 ttgtagatta tattgtaaaa aaaactttaa aaaatcacga tataattaaa aatccatatc
     2041 cacgaatact tgatatttcc tgtgggtgtg gaaattttct tttagaagta tatgatattt
     2101 tatatgactt atttgaagaa aatatatatg aattgaaaaa gaagtatgat gaaaattatt
     2161 ggattgttga taacatacac cgtcatatat taaactattg tatatacggt gctgatattg
     2221 atgaaaaagc tataagtata ttaaaagata gcttaacaaa taaaaaggta gtaaatgatt
     2281 tagatgaatc tgatataaag attaatctat tttgttgtga tagtcttaaa aagaaatggc
     2341 gatataagtt tgattacata gtgggtaatc caccctatat tggtcataaa aaacttgaaa
     2401 agaaatataa aaaatttctc ctagaaaaat atagtgaagt ttataaggat aaagctgact
     2461 tatatttttg tttttataaa aagattatag atattttaaa gcaaggaggg attggcagtg
     2521 ttataactcc aagatatttc ttagaaagtc tatcaggtaa agatttaaga gaatatatta
     2581 aatcaaatgt caatgttcaa gaaatagtgg attttttagg tgcaaatata tttaaaaata
     2641 taggggtatc tagttgtatt ctaacttttg ataagaaaaa aacaaaagag acatatatag
     2701 atgtatttaa aatcaaaaat gaggatatat gtataaataa atttgaaaca ctggaagaac
     2761 ttctaaagag tagcaagttt gaacacttta atataaatca gagattgtta tctgatgagt
     2821 ggatacttgt aaataaagat gatgaaactt tttataataa gatacaagaa aaatgtaaat
     2881 acagcttgga agatatagca ataagttttc aaggaataat tacagggtgt gataaagcat
     2941 ttattttaag taaagatgat gtaaagttga accttgtaga tgataaattt ttaaaatgtt
     3001 ggataaaaag taagaatata aataaatata tagtagataa aagtgaatat aggctaatat
     3061 actcaaatga tatagataat gagaatacaa ataaaaggat actagatgaa attatagggg
     3121 tatataaaac aaaattagaa aacagaagag aatgtaaaag tggaataagg aaatggtatg
     3181 aacttcaatg gggtagagaa aagcttttct ttgaaagaaa aaagataatg tatccttata
     3241 aatcaaatga aaatagattt gctatagatt atgataacaa tttttcaagt gcagatgtat
     3301 attctttttt tataaaagaa gagtatttag ataaattttc atatgaatat ctagttggaa
     3361 ttttaaattc cagtgtttat gataaatact ttaaaataac tgctaaaaaa atgagtaaaa
     3421 atatatatga ttactatcct aacaaggtta tgaagataag gatttttagg gataataatt
     3481 atgaagagat agagaattta tcaaagcaaa taatatcaat tttattaaat aagtctatag
     3541 ataaaggaaa agtagaaaaa ttgcagataa aaatggataa cttgataatg gattctttag
     3601 gtatataagc agttgtaagt attaaaaaat tatactcttt taattgttta tttaaattta
     3661 gaaataaaaa gttagccata taaaaaatat taaaaatata tacttttcaa tatttttttg
     3721 ataaaataag aatgaatttg tgcaatttta tttttctcca ataactaaaa atagtttttt
     3781 gaattaatat ttgcttaaga ataaaaatca gaaacgtttt tgatatagta ggtgataata
     3841 tgaaaggatt agttttagaa ggtggaggaa caaaaggagc ctaccaaata ggtgcatata
     3901 aagctttaag agagcttggt atagagtttc aaggagtaac tggcacatca attggtgctt
     3961 taaatggagc atatataatt caaaatgata ttgaagtaat ggaagatatt tggttaaatt
     4021 atgactatac tcattttatg aatattgata gagaaaccta tgagaggttt aaaaatgtag
     4081 atttcactac taaaaatctc aatgcagtgt ttgagcttat aaataaagct agaaaaaatg
     4141 aaggtataga tataagccca ttaagacaac taatggagac aacattaaat gaagacataa
     4201 taaggaaatc aaaaaaagac tttggacttg ttacggtctg gtgggataaa aagattaatc
     4261 catatccatt gtatatcgaa gatataccta aaggaagatt gattgattat ttaattgcta
     4321 gttccagctt gcctattttt caaacgaata tgatggatga taagttgtat cttgatggaa
     4381 tgttttgtga taacatgcca ataggactct tggaagacaa aggttatgaa gatgttgttg
     4441 taattaggtt gattgatgat tttattggga aaatgaatct aaatcgacat caaaatataa
     4501 atataaaaac tataatacca tcagaatatc taggaggttc tcttaataaa gatagagata
     4561 atgtagaaaa aaacattcga ctaggttact tagatactat gaaagcatat gaaagatgca
     4621 ctggaattaa gtattatttt gatgtagagt ataaatataa tgaagaatat tgttttgata
     4681 aatttagaag tatgagcaat gaaacaatac aatatatctg taatttattg aatataaaaa
     4741 gagatgcttc acttagaact gttatggaaa ttgtaatacc aaagcttggt gaggtgcttg
     4801 gattgccaaa ggatttttca tataaagatt tgttttactg tatatatgaa aagaaacttg
     4861 aagaaaataa tataaacagg ataaaattat atgattttaa caaggttata gatgttgtaa
     4921 atacaaatat tgattctaat agaaatttag cttctaattt tgaagttaga cctaaaatta
     4981 gtattaaaaa gaacaaaaac acaaaattat tgacaaattg tattataaaa gacataaaaa
     5041 atcaaagtta aataaaaaac aaaaaaaaat ttgtaattat actcgaaaaa agtgtatatt
     5101 agtagtataa tatttttgta gcactagtgg gaacaaaaaa tgaccatagg acaaaaaagt
     5161 accacttagt accattaaag taaaaaacag gaaaacaatt ttaaaaaata taaaatgtaa
     5221 agtttatagg aggatttaaa aaatggaaaa agtagttagt atcaaaaatg caagtggatt
     5281 acatgctaga ccagcaggaa tgtttgttaa gaaggcagct gaatttaaat caacagtaga
     5341 ggttgtagca aaagacaaaa ctgtaaatgc aaaatcaata atgggaataa tgagtttagg
     5401 tttagctcaa ggagaagaat taaagttagt tgtaaatgga gaagatgaag aagtagcttt
     5461 agctgcaatg gttgaattag ttgaaagtgg atttggagaa tagatataat aatttttaaa
     5521 aatttgatat ttagaatcta atattactta aagtaaagta acagagaagt attaaacata
     5581 tacagaccaa catttgtaga tatttaattt ctttgttact ttcatgttta tttaatcaga
     5641 agattaagac tattgttaat tgggtataaa aatacaaata ttttagaaaa tgaaataaga
     5701 tatttttaat c
//
LOCUS       DAEPXF010000219         5698 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain HB27
            SAMN13376389-rid12479423.denovo.227, whole genome shotgun sequence.
ACCESSION   DAEPXF010000219 DAEPXF010000000
VERSION     DAEPXF010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13376389
            Sequence Read Archive: SRR10518432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5698)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5698)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001069624.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001069624.1
            Genome Coverage       :: 315x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/15/2021 01:54:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,165
            CDSs (total)                      :: 4,076
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 89
            rRNAs                             :: 10, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 10 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 19 of 69
            Pseudo Genes (incomplete)         :: 44 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 10 of 69
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5698
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13376389-rid12479423.denovo.227"
                     /strain="HB27"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="China: Hebei"
                     /collection_date="2017"
     gene            197..1465
                     /locus_tag="KRL67_003466"
     CDS             197..1465
                     /locus_tag="KRL67_003466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HBH1524307.1"
                     /translation="MEASIHTIASNNLLIIFAIVSITGIICSKLSEIIKVPDVVLYLL
                     VGILIGPSFLKFIDIRGFQIENQLILTFGSAFILYLGGKEISLKVLRNVKISVFLLAT
                     LGVVISAFIMQQVIGFTFGISAMTALLAGTIIASTDPATLVPIFNQVKIKDRVKQTVI
                     SESAFNDATGAILTSAVLAVILSNKFSLGENIYELGTMVIVGVLVGLITGILLLKLVN
                     DKPYGIFKDFAPIISVISVVVAYELSTKLGGSGYMSCFIVGIITGNKKNFKIWLSQKS
                     YDADFYVAETLGTICRMAIFIILGSQVNLAQLSKYFMPSVITVLVLMFIARPLCVLVC
                     TLVDREAKWSKSEILFMMWVRETGVIPAALCGIISAMKVPGYEVISSVVFMTILITLV
                     IQGSTTKLVAKKLGLLEEEVINISEKVSTF"
     gene            1660..2448
                     /locus_tag="KRL67_003467"
     CDS             1660..2448
                     /locus_tag="KRL67_003467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HBH1524308.1"
                     /translation="MFTVMDIVQPDTVEEAYSILNKRKTNQVIGGSAFLRMGKKRIGT
                     GIELSKLNLDYIKEYEDYVEIGCMTTFRTLETSSIIKNNFGRIIEDSVKNIIGVQFRN
                     VVTVGATVFSKYGFSDLIVALLSLDTEVELYNIGRISLEEFLNRDYEKDLLIKIYIKK
                     TNKNASYKSLRNAKSDYPILNVSVSKHMEQFKLCVGARPQKATIAKQASEFLSNNEIN
                     EINIDKAVEIASEELTFGSNMRASRYYRKAMSKVLLKRAIMEVI"
     gene            2448..2897
                     /locus_tag="KRL67_003468"
     CDS             2448..2897
                     /locus_tag="KRL67_003468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster binding
                     domain-containing protein"
                     /protein_id="HBH1524309.1"
                     /translation="MLVKININGKKKSVDIEPEEYLVDTLRKVGNLSVKRGCDTGCCG
                     LCTVLIDKKPTLSCATLTLRALDKEITTIEGLENEVKEFSEVLVKEGAEQCGFCSPGF
                     ILTVLAMKDELENPTDEEIKHYLTGNLCRCTGYMGQLRAIKTYLEVK"
     gene            2901..5165
                     /locus_tag="KRL67_003469"
     CDS             2901..5165
                     /locus_tag="KRL67_003469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HBH1524310.1"
                     /translation="MNIVGKGIKKIDGMSITTGKPVYTDDLSAKDALVVKILRSPHAY
                     AKIKDIDTSKAEMIDGVECVLTYKDVPKSRFTLAGQTYPEPSPYDRLILEDVVRYVGD
                     EVAIVAAIDEKTAIKAMNLIKVKYEVITPVLDFEEAIGNKVVVHDEDRHCNFDIGMQR
                     EKNIVSHHDYTKGDVEKVLSNCDVVIEETYYTQAQAHAMMETYRAYNYLDHTGRLVVV
                     SSTQIPFHVKRHLSRALEIPASKIRVIKPRIGGGFGGKQTACVEIFSAIVTLKTGKPA
                     KIIYDRKETFNCSTSRHAMRLKVRLGATNDGIIKAIDIKALSDTGAYGEHASTTIGLV
                     GEKTLPMYNKVEATRFIADVVYTNKMPAGALRGYGASQGCFAVESTINMLANKLKMDP
                     TDLRLKNVVTEGETTFAYGKILNSVDLIKCINKGKELIGWNEKYPCKHLDNGKIRSVG
                     MAITMQGSGIQGLDKSSAEIKLNDCGDYTLMIGSTDMGTGSDTILAQMAAEVLDTSIE
                     NITVISADTDLVPYDPGSYASSTTYVTGMAVVKAAQDLREKIISAGATKLDVDKDEVE
                     FDGIKLTSKKGSTLLFDIAVDSTIGPDTNQLIGLASHGSPVSPPPFIAGFAETEIDTE
                     TGKVDLVNYVAVVDCGTVINKNLAKIQVEGGIVQGIGMALFEEVRYTEKGSMDTNTFM
                     QYKIPARCDIGNVKVDFVETYEPTGPFGAKSVGEVVINTPLPAIQEAVFNGIGVRINT
                     LPITPEKVFMEMNK"
     gene            5305..5616
                     /locus_tag="KRL67_003470"
     CDS             5305..5616
                     /locus_tag="KRL67_003470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH1524311.1"
                     /translation="MAGNLNNMRAVNNFRGDKNILECLVSFEGRSISQRKVRVFFKEK
                     QNQIEIDFAEEEISKLVENVVLNTSYQEMLYDEIEKQLEIDCIGTWMILSKLKDGSRV
                     H"
BASE COUNT         2175 a          606 c         1092 g         1825 t
ORIGIN      
        1 aaacttgaca aaatcaacct tttaataact agcaaaaaat agtaaaatta tataagcgtt
       61 atttaattgt caaaaatgaa agctaaattg catgtcctaa aaaaaatgat tgataacatt
      121 tatttgtgtg ctataattat atagtaaagc aataagtata ttgcataatg tatgcaaatt
      181 gggaggagag acggacatgg aagcatctat acacacaatt gccagcaata atttattgat
      241 aatttttgct attgtaagta taactggaat aatttgtagt aagcttagtg aaataataaa
      301 ggttccagat gtagtgttat atttacttgt aggtatttta ataggaccat catttttaaa
      361 atttatagat attcgtggat ttcaaataga aaatcaacta atacttacat ttggttctgc
      421 atttatttta tatttagggg gaaaagaaat aagtttaaaa gtattgagaa atgtgaagat
      481 atctgtattc ttattagcta ctttaggagt tgtgatatct gcttttataa tgcaacaagt
      541 aataggattt acttttggga taagtgcaat gactgcttta ctagcaggaa ctataatagc
      601 atcaacagac ccagctactc ttgtacctat atttaatcag gtaaagataa aggatagggt
      661 aaaacaaact gtaataagtg aatcagcttt taatgatgca acaggagcta tactaacatc
      721 agcagtgttg gctgtaattt tgtcaaataa attttcatta ggtgaaaata tttatgaact
      781 ggggacaatg gttattgtcg gagttttagt tggtttaatt acaggaatac tgcttttgaa
      841 attagttaat gataaacctt atggaatatt taaagatttt gcaccaataa tatctgttat
      901 ttcagttgta gttgcatatg aattatctac aaaattaggt ggaagtggat atatgtcttg
      961 ctttattgta ggtataataa ctggtaacaa gaaaaacttt aaaatttggt taagtcaaaa
     1021 atcttatgat gcagattttt atgttgctga aactttaggt actatttgtc gtatggcaat
     1081 atttataata ttgggtagcc aagttaattt agctcagtta agtaaatatt ttatgccatc
     1141 tgtaattaca gtattagtat taatgtttat agcaagacca ctttgcgttt tagtttgtac
     1201 gcttgtagac agagaagcaa aatggagtaa aagtgaaata ttatttatga tgtgggtaag
     1261 ggaaacagga gtaataccag cagcactttg tggtattatt tcagcaatga aagttcctgg
     1321 atacgaagta atttcatctg tagtatttat gactatacta attacattag taatacaagg
     1381 aagcactaca aaattagtag ctaaaaaact tggattatta gaagaagaag tgataaatat
     1441 aagtgaaaaa gtatcaactt tttaaagttt tgctttaaaa tagtatctgc taatggctat
     1501 tttaatattg tctaatgatg attattgaaa tagtcatttt tttatgtata agtaatttac
     1561 agaatttaca tattagacat atttattatc aaaatttgtt gaagctatga tataattagt
     1621 tgtatttaaa ataagaatag caaataaggg aggaaattta tgtttacggt tatggatatt
     1681 gtacagccag atacagtaga agaagcgtac tcaattttaa ataaaagaaa aactaaccaa
     1741 gtaattggtg gaagtgcttt tttaagaatg gggaaaaaaa gaataggaac aggaatagaa
     1801 ttatctaaat taaatttaga ttatataaaa gaatatgaag attatgttga gattggttgt
     1861 atgactactt ttagaacatt agaaacaagt tccataataa aaaataattt tggaagaata
     1921 atagaagatt cagttaaaaa tataataggg gttcagttta gaaatgtagt aacagttgga
     1981 gcaacagtgt tttctaaata tggtttttct gacttgatag tagcattact ttcattagat
     2041 acggaagtcg aattgtataa tattgggaga attagtcttg aagagtttct aaatagagac
     2101 tatgagaaag atttacttat taagatatac ataaagaaga caaataagaa tgcttcttat
     2161 aaatcactta gaaatgcaaa gagtgattat ccaatcttaa atgtatctgt atcaaaacat
     2221 atggaacaat ttaagttatg tgtaggagca agaccgcaga aagctacgat tgcaaaacaa
     2281 gctagtgaat ttctatctaa caatgaaatc aatgaaatta atatagataa agcagtagaa
     2341 atagcatcag aagaactaac ttttggttct aacatgagag catctaggta ttatagaaaa
     2401 gctatgagta aagtactttt aaaaagagct ataatggagg ttatttaatg ttagtgaaga
     2461 ttaatataaa tgggaagaaa aaaagtgttg atatagaacc agaagaatat ctggttgata
     2521 ctttaagaaa agttggtaat ttaagcgtga aaagaggttg tgacacaggc tgttgtggtc
     2581 tatgtacagt tttaattgac aagaaaccta cattatcttg tgcaacactg acattgagag
     2641 ctttagataa agaaataact accattgaag gtttagaaaa tgaagttaaa gaatttagtg
     2701 aagttttggt aaaagaagga gctgagcaat gtggtttctg ttctcctggc tttatactta
     2761 ctgtacttgc aatgaaggat gagcttgaaa atcctacaga tgaggaaata aagcattacc
     2821 ttacaggaaa tctatgtaga tgtacaggat atatgggaca gttaagagct atcaaaactt
     2881 atctggaggt aaaatagaac atgaatatag ttggaaaagg aatcaagaaa atagatggaa
     2941 tgagtatcac aacaggaaaa cctgtatata cagatgattt atcagcaaaa gatgctttgg
     3001 tggtaaagat acttagaagt cctcacgcct atgcaaaaat aaaagatata gatacatcaa
     3061 aggcagaaat gattgatggt gttgaatgtg ttttaacata taaagatgta ccaaaatcta
     3121 gatttacact tgctggtcaa acatatccag agccatctcc atatgataga ttaattcttg
     3181 aagatgttgt aagatatgta ggtgatgaag tagctatagt ggcagctata gatgaaaaaa
     3241 ctgcaattaa agcaatgaat cttatcaaag taaagtatga agtaattaca cctgttttgg
     3301 attttgaaga ggcaatagga aataaagttg tggtacatga tgaagataga cattgtaatt
     3361 ttgatatagg tatgcaaaga gaaaaaaata tagtatctca tcatgattat acaaaaggtg
     3421 atgtagaaaa agtattaagt aattgtgatg tagtgattga agaaacttat tatactcaag
     3481 ctcaagcaca tgcaatgatg gaaacgtata gggcatacaa ttatttagac catacaggaa
     3541 gattggttgt agtaagttca actcaaatac catttcatgt aaaaagacat ttatctcgtg
     3601 cactagaaat accagctagc aaaattaggg ttataaaacc tagaattggt ggcggatttg
     3661 gtggtaaaca aactgcatgt gttgagattt tttctgcaat tgttacttta aaaacaggga
     3721 aaccagctaa aataatttat gatagaaaag aaacttttaa ttgttcaaca agtagacatg
     3781 caatgagatt aaaagttaga ttaggtgcta caaatgatgg cataataaag gcaatagata
     3841 taaaagcact ttctgataca ggagcatatg gagagcatgc ttctacaact attggtttgg
     3901 ttggagaaaa aactttacca atgtataata aagttgaagc aacaagattt attgcagatg
     3961 tggtatatac taataagatg ccagcaggag cacttagagg gtatggagca tcacaagggt
     4021 gttttgctgt tgaatcaact ataaatatgc ttgctaataa attaaaaatg gacccaacag
     4081 atttaaggtt aaaaaatgtg gtaacagaag gtgaaactac atttgcatat ggtaaaattt
     4141 taaatagtgt cgatttaata aaatgtataa ataaaggcaa agaacttatt ggatggaatg
     4201 aaaaatatcc ttgtaaacat cttgataatg gaaaaattag aagtgtgggt atggcaatta
     4261 ctatgcaagg ttctggtata caaggtcttg ataagtcttc agctgaaata aaattgaatg
     4321 actgtggaga ttatactctt atgataggtt ctactgatat gggtacagga agtgatacaa
     4381 ttttagctca aatggctgct gaggttcttg atacaagcat tgaaaatata acggttatat
     4441 cagctgatac tgatttagtt ccatatgacc caggttcata tgcttcaagt actacttatg
     4501 taacaggtat ggcagtagtt aaggcagctc aagacttaag agaaaaaata atttctgctg
     4561 gagcaactaa actagatgtt gataaggatg aagtagaatt tgatggtata aaattaacta
     4621 gtaaaaaagg tagcacttta ttattcgata tagcagttga ttctacaatt ggtccagata
     4681 ctaatcaatt aataggtctt gcttctcatg gtagcccagt atctcctcca ccttttatag
     4741 caggttttgc agaaactgaa attgacacag aaactgggaa agttgattta gtaaattatg
     4801 tagctgtagt agattgtgga acagttataa ataaaaatct agctaagata caagttgaag
     4861 gtggaattgt tcaagggatt ggaatggcat tatttgaaga agttagatac acagaaaaag
     4921 gaagcatgga cacaaataca tttatgcaat acaagattcc agctagatgt gatattggaa
     4981 atgttaaagt agattttgtt gaaacttatg aaccaactgg gccatttggt gctaaatctg
     5041 ttggagaagt tgttataaat acacctttac cagcaataca agaagctgtg tttaatggaa
     5101 ttggtgtaag aataaataca cttcctatta cacctgaaaa agtttttatg gaaatgaata
     5161 aatgattatt tatgaaaatt tagactagtt ttacatgtta aacaaccagt tatgtcatac
     5221 ctgttgaaag atatttaata agtagttatt ataatattat gaattttact tataaaatac
     5281 aaaattaaat aaggggatga tgttttggct ggtaatctaa ataatatgag agcagtaaat
     5341 aattttagag gagataagaa cattttagaa tgtttagtca gctttgaggg tcgttcaata
     5401 agtcagagaa aagtaagggt attttttaaa gaaaaacaaa atcaaataga aattgatttt
     5461 gcagaagagg aaatttctaa attggttgaa aatgttgttt taaatacatc atatcaagaa
     5521 atgttatatg atgaaataga gaaacaactg gaaattgatt gtataggtac ttggatgata
     5581 ttatctaaat taaaagatgg tagtagagtt cactgatttt aaaataaatt attagagcct
     5641 aattttaagg ctcttttttg ctgtttttaa ttaacaatag aaaatttatc tttaattt
//
LOCUS       DAEPXF010000220         5660 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain HB27
            SAMN13376389-rid12479423.denovo.228, whole genome shotgun sequence.
ACCESSION   DAEPXF010000220 DAEPXF010000000
VERSION     DAEPXF010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13376389
            Sequence Read Archive: SRR10518432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5660)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5660)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001069624.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001069624.1
            Genome Coverage       :: 315x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/15/2021 01:54:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,165
            CDSs (total)                      :: 4,076
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 89
            rRNAs                             :: 10, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 10 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 19 of 69
            Pseudo Genes (incomplete)         :: 44 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 10 of 69
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5660
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13376389-rid12479423.denovo.228"
                     /strain="HB27"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="China: Hebei"
                     /collection_date="2017"
     gene            41..1783
                     /locus_tag="KRL67_003471"
     CDS             41..1783
                     /locus_tag="KRL67_003471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M3"
                     /protein_id="HBH1524312.1"
                     /translation="MVKSKKKIFVSLILIAVFIIFASYIRNYTNDTKKDSVRGTIPFE
                     KMEYKRPNIQSICKNINKCNDKLLTAKTAKEQLNLFNEVDKIYQDFYSTLTISKIRNN
                     IDSSDEFYSKEYKYLMSKSVDVDMSYKDFQDRFVTSKFSKELEKELGKDTFNYLKNIG
                     KLNSKEVEGLLKKEQDLVVKYEDLLAKSTVSIDGIEVDFEEALSRPNLSPEEYVKIYS
                     DYLKKYNPIFGNIFLELIQTRTEIASKQGFKNYIDYAYMNLNKDYSQKEAKKFRQDVK
                     DYIVPLYREISSKPSDSSIYIKVYKNRSFRKFDTVLEDISPKLKESFDYMKKYDLYDY
                     SSGKNKSPGGYTTYINKYKAPFLFNTWDNSFLGVTSFAHEFGHFYNYYNSINLNNKMQ
                     PSIDVCEVHSTSLEILFYKYFDDFFGKQSEAIKKEHLSIVLNTIIDACLYDEFQEIIY
                     KNPYMSLNDINKLFFNLEEEYGVSNNILKEKNAPFWVLVSHNFQVPFYYLSYGLASDV
                     SLQIWQLSQEDYRKAVDVYMDFLNQNTDAGFKDVVEKVNLQLPFQDGNLEEISSTLYD
                     YFGIDNPLELKNAS"
     gene            complement(1947..2141)
                     /locus_tag="KRL67_003472"
     CDS             complement(1947..2141)
                     /locus_tag="KRL67_003472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH1524313.1"
                     /translation="MKIITIGSGLITVLLFLSTMVCGFWIKNNKVTDASSIKFHMNSA
                     IFTGIFLLISTILLIIYIKK"
     gene            complement(2138..2719)
                     /locus_tag="KRL67_003473"
     CDS             complement(2138..2719)
                     /locus_tag="KRL67_003473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBH1524314.1"
                     /translation="MKSNDKDARERIIEVTLNLLNEVDDIEEITVRKIAERANVGVGL
                     INYHFKTKDNLLSTAIGDVMSNIIAELYDDSVYTLRPIEDLKNLLKKLCDTGLHYEKV
                     LPFVLNQCIANGDMQAELDIVPMLRKIFGNKKDEMSLRIIALQIILPIQISALSTESF
                     QLYSGINIKNKYERDKFIDILIENIIGEDVDVR"
     gene            2887..3705
                     /locus_tag="KRL67_003474"
     CDS             2887..3705
                     /locus_tag="KRL67_003474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HBH1524315.1"
                     /translation="MFKIGDFSKLSKISIRMLRHYDEIGLLTPSHTNKTNGYRYYSAD
                     QLSTTNRIHALKDMGFGLYSIKEILTEYNDKESLIKYLNIHHSQVKEQLEDTQKKLLK
                     IENTIKRIGGNDIMKNYDVTIKNFASKYMMTLRMVIPTYQDEGMLWHQAFLETKDQNV
                     QIEPPKHSKAVFYDTGYKEDYVDVEVQVAVSGKYKDTEHVKFKAVPSVTAATAIVNGN
                     FNQVADACEAIGNWISDNNYEVDGPMFNIYHVSPGNDNNPDNWVTEVCFPVKKK"
     gene            4577..5143
                     /locus_tag="KRL67_003475"
     CDS             4577..5143
                     /locus_tag="KRL67_003475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH1524316.1"
                     /translation="MKLKIVGSGGMSIIPSSFCKCKICEEARQKGGRYERLGPSLYID
                     DIKMLIDTPEDIAVACNRQKISEVKHISISHHDPDHVKGIRIVEKIGCDFITGENNPI
                     GFYALPEVIEDINRTNLDCLKYYGDVINCISINETSHVRINNFDIDLINNNPDRNITF
                     YVIKENEKKVIYACCNPKPFTHSDMYFD"
BASE COUNT         2173 a          740 c          795 g         1952 t
ORIGIN      
        1 taaaataatt aataactata tccatgattg gaggattttt atggttaaat ccaaaaagaa
       61 aatatttgtt tctctaattt taattgcagt ttttattata tttgcatcat acataagaaa
      121 ttatactaat gatacaaaaa aagattctgt tagaggaact attccttttg aaaaaatgga
      181 atataagcgt cctaatatac agtcaatttg caaaaatatt aacaagtgta acgacaaact
      241 tttaacagct aaaacagcta aagaacaatt aaatttattt aatgaagttg ataaaattta
      301 tcaggatttt tacagtacac ttactatatc aaaaattaga aataacatag attcatcaga
      361 cgaattttac tctaaggaat ataagtatct tatgagtaaa tcagtagatg ttgacatgtc
      421 atacaaagac tttcaagata gatttgtaac ttctaaattc tctaaagaat tagaaaaaga
      481 acttggaaag gatacattta attatctaaa gaacattgga aaacttaatt ctaaagaagt
      541 tgaaggtttg cttaaaaaag agcaagacct agttgtaaaa tatgaagact tattagctaa
      601 atctacagta tcaatagatg gtatagaggt agactttgaa gaagccttgt caagaccaaa
      661 tcttagccct gaagaatatg tgaaaattta ttcagattac ttaaaaaaat acaatcctat
      721 atttggtaac atattcttag aacttataca gacaagaact gaaatagcaa gtaagcaagg
      781 atttaagaat tatatagatt atgcctatat gaacctaaac aaagactatt ctcaaaaaga
      841 agctaaaaaa tttagacaag atgtaaaaga ttacatagtc ccactatata gagagatttc
      901 atctaaacct tctgattctt ctatttatat taaagtatat aaaaatcgct catttaggaa
      961 gtttgatacg gtacttgaag atatatctcc aaaactaaaa gagagtttcg actatatgaa
     1021 aaaatatgac ttgtacgatt attcttctgg aaaaaataaa tcgcctggag gatatactac
     1081 ttatataaat aaatataaag cacctttctt gtttaataca tgggacaatt cttttttagg
     1141 tgttactagt tttgctcatg agtttggtca tttttataat tactacaaca gtattaactt
     1201 aaataataag atgcaaccaa gtatagatgt atgtgaagtt cattcaacat ctttagaaat
     1261 tctattttac aaatattttg atgatttttt tggtaaacaa tctgaagcta taaaaaaaga
     1321 acacttatcg attgttttaa atactattat tgatgcttgt ctttatgatg aatttcaaga
     1381 aattatatac aagaatccat atatgagtct aaatgatatc aataaattgt tttttaattt
     1441 agaggaagaa tatggagttt ccaataatat acttaaagaa aaaaatgctc ctttttgggt
     1501 acttgtatct cataacttcc aagttccttt ttattacttg agttatggtc ttgcatctga
     1561 tgtatcactt cagatatggc aattatcaca agaagattac agaaaagctg ttgatgtata
     1621 tatggatttt ttaaatcaaa atactgatgc tggatttaag gatgtggtag aaaaggtgaa
     1681 tttgcaatta cctttccaag atggaaattt agaggaaata agctctactt tatatgatta
     1741 ttttggtata gataatccac ttgaactaaa aaatgcttct taatttttaa tactttatgc
     1801 tcactcttaa gatttagtat tagattgaaa taagtgatat tttttcacac acaattaatg
     1861 tttaataaaa ttatatggta aagtatcaga aatttttaag actcttgtct ttataatttc
     1921 tgatacttta tctattaata atattattat ttttttatat atataattaa aaggattgtg
     1981 gaaatcaata aaaatatccc tgtaaaaata gcactattca tatggaactt aatagaactt
     2041 gcatctgtta ccttattatt cttaatccag aacccacaaa ccatagttga aagaaaaagt
     2101 aaaacagtga taagacctga accaatagtt ataattttca tcttacatca acatcctctc
     2161 caataatatt ttctattaaa atatcaataa atttatctcg ctcatattta ttttttatat
     2221 ttataccact gtataactga aatgattctg tagataaagc agaaatctgt attggtaaaa
     2281 taatttgcag ggcaataatt cttagactca tttcatcttt tttatttcca aaaattttac
     2341 gaagcatagg gacaatatca agttctgctt gcatatctcc attggctata cattgattca
     2401 acacaaaagg aagcactttt tcataatgta atccagtgtc acacaatttt tttaataaat
     2461 ttttcaagtc ttcaataggt cttagagtat atacactatc gtcatatagc tctgctatta
     2521 tatttgacat cacatctcct atggcagtac tcaacaaatt atcctttgtt ttaaaatgat
     2581 aatttattaa accaactcca acatttgcac gctcagcaat ttttctaact gtaatctcct
     2641 caatatcatc tacttcattt aaaagattca atgtaacttc aattatacgt tctctagcat
     2701 ctttatcatt acttttcata atagacctcc aaaacaatta acattttcag ttctgaacac
     2761 gttcagtata atatgttttt catcatatgt caatataatt taattataat tttagttgac
     2821 tctaacatta tgatatagtt tatattaata ttaagctaaa agtaccttta taaaggagac
     2881 aaaaacatgt ttaagatagg cgatttttca aagctaagta aaattagtat tcgtatgtta
     2941 cgtcattatg acgaaatagg gcttttaact ccttctcata cgaataaaac taatggatat
     3001 agatactata gtgcagacca actttctact acaaacagaa tacatgcact aaaagatatg
     3061 ggctttggct tgtattctat aaaagaaatt ttgacagaat ataacgataa agaaagtctt
     3121 ataaaatatc taaatatcca ccattctcaa gtaaaagaac aattagaaga tacacagaaa
     3181 aaactattaa aaatagaaaa cactattaaa agaattggag gaaatgatat tatgaaaaat
     3241 tatgatgtaa ctataaagaa ttttgcatca aaatatatga tgacacttag aatggttata
     3301 ccaacttatc aagacgaagg aatgttatgg catcaagcat ttttagagac aaaagaccag
     3361 aatgttcaaa ttgaaccacc aaagcattct aaggcagtct tttatgatac tggatataaa
     3421 gaagattatg tagatgttga agtacaagtt gctgtatctg gaaagtataa agatactgag
     3481 catgttaaat ttaaggctgt tccaagtgtg actgctgcaa ctgcaattgt taatggaaat
     3541 ttcaatcaag ttgcagatgc ttgtgaagct attggtaatt ggatatcaga taataactat
     3601 gaggtggatg gtcctatgtt taatatttat catgtaagtc ctggcaacga taataatcct
     3661 gataattggg taacagaggt atgcttccca gttaaaaaga agtaatttta tcttatatat
     3721 taattggata atattttaat ttgttagaca aattactttt agtgttatgt ataaatacct
     3781 acaatttaat taggatatta tcttaatttg tcagataaat cacttgtgat gttatgttta
     3841 aatacctaca atttaattag gatattatct taatttgaca gataaatcac ttgtgatgtt
     3901 gtgtttaaat gactagaatt ttattaggat attatcttaa tttgtcagat aaattattta
     3961 taatgttatg tttaaatacc tacaatttaa ttaggatatt atctcaattt gtcagataaa
     4021 tcaattatga ttttatgttt aaatgactag aatttgatta ggatattatc ttaatttgtc
     4081 agataaatca attataagat tatatactat ccttatactc tagttaaata tattttaatc
     4141 actaatataa attagttttt ttaacaaaac aataaaagag taaagtacaa ataattcctt
     4201 tgtgctctac tctttttaac tttctttaga caatgataca atgataaata taaaaaatta
     4261 tatattccat tctatagaaa tatgctcacc aataacatgt atgctgttaa tcaatatatt
     4321 atatcaaaat tacataaaac ttataggaca atcatatctt attacaagaa aaatgtctta
     4381 tccatattga ataagtcatt cagttcatat acaaagtcaa tttcattgag aaacatttaa
     4441 tatatgttta taaaaatttc atactattct ttcatatttg catgtgatat aatttattaa
     4501 ctcataattt tgttaatcag ttgtgcttgt agattattta tataatctaa tattttaaga
     4561 aggtggtgta ataactttga aattaaaaat tgttggtagt ggcggtatgt caattattcc
     4621 aagttcattt tgcaaatgca aaatatgtga agaagccaga caaaaaggcg ggagatatga
     4681 acgattaggg ccaagtttat atattgatga tataaaaatg ctgatagaca cacctgaaga
     4741 cattgcagta gcttgtaaca gacaaaaaat atcagaagta aaacatattt caatttctca
     4801 ccatgaccca gaccatgtta agggtattag aattgtcgaa aaaataggat gtgactttat
     4861 cacaggagaa aataatccaa ttggctttta tgcgcttcct gaagtaattg aagatataaa
     4921 ccgaacgaat ctcgattgtc ttaaatatta tggtgatgtt ataaattgca tttctattaa
     4981 tgaaacttca cacgtaagga taaataattt tgatattgat ttgattaaca ataatccaga
     5041 caggaatata accttctatg ttatcaaaga gaatgaaaaa aaagttatat acgcctgctg
     5101 taatcccaag ccatttactc atagtgatat gtactttgat taaaaaatag ctacgcacac
     5161 ttgtgacttt agtcatgagt tagtagctaa attagtcagc atatagggaa acttatatgt
     5221 agagatagga tagaacctat ccaatactac ttgaattgct aaaaacccct aaagctaatc
     5281 aaactaaaac ataaggatga aataaaccta agtgtgaaag ttacgaaagt agaaaaaatt
     5341 gattagatgg tataaggtta aaccctaagt actttaaaat gggcaatcag caggtaaact
     5401 ccgaatagga gaaacttcaa cgactattcc tcttgaggga agtacactac aagctattgg
     5461 tagtggaagt gggtagacct tagcggataa tgccgaagga taagatatag tctgtgctta
     5521 catgaaaatg taagaagttc ataagagaac tgcataggta gtagcgaact tatgtgaacg
     5581 acaacctcaa aaatgataaa agttaagact tacggttctt aaatatgtta tttaaaatta
     5641 aatataaaac cttctaaata
//
LOCUS       DAEPXF010000221         5652 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain HB27
            SAMN13376389-rid12479423.denovo.229, whole genome shotgun sequence.
ACCESSION   DAEPXF010000221 DAEPXF010000000
VERSION     DAEPXF010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13376389
            Sequence Read Archive: SRR10518432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5652)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5652)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001069624.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001069624.1
            Genome Coverage       :: 315x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/15/2021 01:54:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,165
            CDSs (total)                      :: 4,076
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 89
            rRNAs                             :: 10, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 10 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 19 of 69
            Pseudo Genes (incomplete)         :: 44 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 10 of 69
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5652
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13376389-rid12479423.denovo.229"
                     /strain="HB27"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="China: Hebei"
                     /collection_date="2017"
     gene            complement(<1..685)
                     /locus_tag="KRL67_003476"
     CDS             complement(<1..685)
                     /locus_tag="KRL67_003476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBH1524317.1"
                     /translation="MLTQMNFYHNKKVKVSIENVSKAYKDIEVLKDISIDVHEGEFVS
                     ILGPSGCGKSTIFNIITKLTDYDSGKVNINGKYSYMYQKDLLLPYKTIIDNVSLPLVL
                     KKEKKSKARSMVKPYFEVFGLSGYEDKYPSELSGGMRQRANFLRTFINSNDIMLLDEP
                     FGALDSLTKTSMQKWLLDVKKKVNSTILLITHDIDEAIMLSNRIYVISKKPSIVKKEF
                     VIDSRKLNED"
     gene            complement(654..1667)
                     /locus_tag="KRL67_003477"
     CDS             complement(654..1667)
                     /locus_tag="KRL67_003477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBH1524318.1"
                     /translation="MNFKKLGILGLVSVFTLATITGCSSNNNKDKNNDKDTSSKKVTM
                     VLDWTPNTNHTGLFVALDKGYYKEEGLDVEIVQPPESGAETLVATGKADFGISYQEQV
                     TYAKTSEDPLPIKAVATVIQHNTSGFASPKEKNITTAKDFEGKTYGGWGSPSEEAVFK
                     AVMKKNGADFNKLKIVNTGQDDFFAAMKTVDFAWIFEGWDAVKADLIGYDLNFIPVKD
                     LDERLDYYTPLIISNETVLKDNPELAKKFLKATTKGYEYAIKNPEESAKILVKHAPEV
                     DEKLALKSQEYLASKYKDDAPRWGEMKDSVWNNYTSFLKEYKLIDKDMKASDAYTNEF
                     LPQ"
     gene            complement(1689..2459)
                     /locus_tag="KRL67_003478"
     CDS             complement(1689..2459)
                     /locus_tag="KRL67_003478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBH1524319.1"
                     /translation="MKNLEIIDKVKDSIYPIATFLFILILWQSMVGVLEVPQYILPTP
                     VDIINVFFKDYQNLSMHAVVTIGEAILGFIVAIIISLVIGILMDFVSIIKKCLYPIML
                     VTQMIPTIIIAPLFMIWFGFGTMPKVLMVMLTCFFPILISFVDGIENIDKDYLNLFKT
                     MNSNKVNTFIHLKFPMAMDKFFSGLKISATYAVMAATVAEWLGGTKGLGVYMLRSKSA
                     YALDKVFASTILVVIFSLMFVGIVQAVKKVVIRHRLID"
     gene            complement(2434..2724)
                     /locus_tag="KRL67_003479"
     CDS             complement(2434..2724)
                     /locus_tag="KRL67_003479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MTH1187 family thiamine-binding protein"
                     /protein_id="HBH1524320.1"
                     /translation="MVIADIAVMPLRPYKGEEEMYKVVDACIAEIEKSGLKCEIGAMS
                     TTVEGDFDEVFELLKKVHKIPFNLGCERVITVARVDEKAGGLTIDEKLRNHR"
     regulatory      complement(2855..2954)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     regulatory      3572..3661
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            3714..3890
                     /locus_tag="KRL67_003480"
     CDS             3714..3890
                     /locus_tag="KRL67_003480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH1524321.1"
                     /translation="MFMDKMDRCTHILTAYICSSRDYCNFIDTQLNDFILEYGENVVE
                     SCLHQVMVLVSRYN"
     gene            complement(4004..4360)
                     /locus_tag="KRL67_003481"
     CDS             complement(4004..4360)
                     /locus_tag="KRL67_003481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBH1524322.1"
                     /translation="MDIDNNFEEFPNIEERQKDFNCSIGFTMTVIGSKWRAIILWHIL
                     KQEPIRYSQLKKEVVHISHKILSQELKYLERDGLIKRISYPTIPPKVEYLSTKRGKSL
                     ESILLELCNWGKQYMR"
     gene            4616..5536
                     /locus_tag="KRL67_003482"
     CDS             4616..5536
                     /locus_tag="KRL67_003482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family isomerase"
                     /protein_id="HBH1524323.1"
                     /translation="MNKKFRLYQVDSFTKEKFTGNPAGVITNADGLSESQMQKIAREL
                     NNSETAFIFNKLAKNHQENYDVHIRFFTPTNEVPICGHATIAAHYVRAVENQFNNKTV
                     FQKTGAGILPVNIIKKDNDYEIIMTQGKIEVSPPLQQTYQKRLLNAFGILKEDIRTDC
                     PIAISSTGHSKVMIGIKELSTLNSLSPNLYELSKISQEIKCNGYYIFTLHDKKTLQVH
                     GRMFAPAIGISEDPVTGNANGPLGAYLVHYKVIPIPNTEFIFEAVQGEAIKRKGKMLV
                     KVIISDNKPVEVKIIGKAVIAFSTNIEIEL"
BASE COUNT         1964 a          895 c          661 g         2132 t
ORIGIN      
        1 tatcttcatt taactttcta ctatctataa caaattcttt ctttactatt gatggttttt
       61 ttgaaataac ataaattctg tttgatagca ttatagcttc gtcaatatca tgagttataa
      121 gaagtattgt tgaatttact tttttcttaa catctaaaag ccatttttgc atggatgttt
      181 tagttaatga atcaagtgcc ccaaaaggct catctaaaag cattatgtca ttagaattta
      241 taaaagttct caagaaatta gctctctgtc tcattcctcc agaaagttct gaaggatatt
      301 tatcttcata tccgcttaat ccaaatacct caaaataagg ttttaccatt gacctagctt
      361 tactcttttt ttcttttttt aaaaccagtg gtagtgacac attatctatt atagttttat
      421 atggcaagag taaatctttt tgatacatat aactatattt accatttata ttcacttttc
      481 cactatcata atctgtaagt ttagttataa tattaaaaat agtacttttt ccacatccac
      541 ttggccctaa tatagaaaca aattcacctt catgaacatc aatgcttata tcttttaaaa
      601 cttcaatatc tttataagct tttgatacat tttctataga tacttttact tttttattgt
      661 ggtaaaaatt catttgtgta agcatcactt gccttcatat ccttatctat taatttatat
      721 tcttttaaga atgatgtata gttgttccat acactatctt tcatttctcc ccatcgtggt
      781 gcatcatctt tgtacttgct tgctaaatat tcttgactct ttaaagctag tttttcatct
      841 acttcaggtg catgttttac taaaattttt gctgattctt ctggattttt tattgcatat
      901 tcataacctt ttgttgtagc ttttaagaat tttttagcaa gctctggatt atctttaagt
      961 acagtttcat tagaaattat aagtggtgta taataatcca atctctcatc taaatctttt
     1021 actggtataa agtttaagtc atatccaatt aaatcagctt taactgcatc ccaaccttca
     1081 aatatccatg caaaatctac agttttcata gctgcaaaga aatcatcttg accagtattg
     1141 actattttta acttattaaa atcagctcca tttttcttca taactgcttt aaaaacagct
     1201 tcttctgatg gagagcccca accaccataa gtttttcctt caaaatcttt agctgttgtt
     1261 atattctttt cttttggtga tgcaaaccca gaagtattat gttgtataac agttgctaca
     1321 gcttttattg gaagtggatc ttcactagtc tttgcataag taacttgttc ttgatagctt
     1381 ataccaaagt cagcttttcc tgttgctact aaagtttctg ctccactttc aggtggttgt
     1441 actatttcaa catccaaacc ttcttcttta tagtaaccct tatcaagtgc tacaaataat
     1501 ccagtatgat ttgtatttgg tgtccaatca agcaccatag ttactttttt agaagaagta
     1561 tctttatcat tgtttttgtc tttattatta tttgaagaac atccagttat tgttgccaat
     1621 gtaaaaacac ttactaaacc taatattcct aactttttaa aattcataat tgcctccctc
     1681 aaataccttt aatctattaa tctatgtctt ataacaactt tttttacagc ctgtacaatt
     1741 cctacaaaca ttaaactaaa tataactact aatatagtag atgcaaatac tttatcaagt
     1801 gcatatgcac tttttgacct aagcatgtaa acaccaagtc ctttcgttcc acctagccat
     1861 tctgcaacag ttgctgccat tacagcatac gttgcagata ttttaagtcc agaaaaaaat
     1921 ttatccattg ccataggaaa tttaaggtga atgaatgtat taactttatt tgagttcata
     1981 gtcttaaata aatttaaata atctttatca atattctcaa tgccatctac aaaacttatt
     2041 aatataggaa aaaaacaagt aagcattacc atcaatactt ttggcattgt tccaaatcca
     2101 aaccatatca taaaaagtgg tgctataatt attgtgggta tcatctgcgt aaccaacata
     2161 ataggataaa gacatttttt tattattgat acaaaatcca tcaatattcc aattaccaat
     2221 gatattataa ttgcgactat aaaaccaagt attgcttctc ctatagtaac cacagcatgc
     2281 atggataaat tttgataatc tttaaaaaac acattaatta tatctactgg tgtgggtagt
     2341 atatactgag gaacttctaa aactcctacc atagattgcc aaagaataag tataaacaaa
     2401 aatgttgcta ttggatatat actatctttg actttatcta tgatttctaa gtttttcatc
     2461 tatagtcaga cctccagctt tttcatctac tctagctact gttatgactc tttcacatcc
     2521 aaggttaaaa gggattttat gtaccttttt taataattca aaaacttcat caaaatcacc
     2581 ttcaacagta gtgctcattg ctcctatttc acatttaaga ccactttttt caatttcagc
     2641 aatacaagca tctactactt tatacatttc ttcttcacct ttgtaaggtc ttagtggcat
     2701 tactgctatg tctgcgatta ccatttaaat cactccttta aaaataatta atacctacta
     2761 ggtatatttt gttttttgtt aaaaaatttt attactaaaa agatattata tttaaataaa
     2821 agaaaagtct tatccaaaat aggataagac ttatttttat cttaactggt ctccctacac
     2881 tggcattatc caaacaggtt caaagggtcg agataaaatc tcctctcagc acattagctc
     2941 cccgaaatat attatatctt ttctacttga ttatactaca cttatcatat tttaacaaat
     3001 tttttattta tattagatat tttatccatt caaaaatatc taatataaat tgcacattat
     3061 tacatcaaat aaattatctt aaaacagtaa tttatcagta tcttccaaaa ctagattaac
     3121 tgaattatct agtttttatt ttgctatttc aagcatattt ggatgtatat caattccata
     3181 caaatattgt actaaatttc tgttttcact catctaattt gcaatcgtta cagattcagg
     3241 ttagacatag cttttagact ctacttatct ttatctacaa aaaatccttc aataaattca
     3301 ttaataatat tcattaattt aaaaataaac ctctatctga atttttaaga tacaagcctt
     3361 tcaggagtat cttttgtgag aatataaaaa ttactcgtca ttatcatcaa aatcctcagc
     3421 aaccaacatt ttagataaaa atcaaattct ataatataat tattttatcc aatttatttt
     3481 tgagaaataa aatatttttt taatttttaa attttttaat atgcaccttg aaaaaagaaa
     3541 cgttttccta tattatggta atagtagaat aaaactaaat aggcaaaact agagaaattt
     3601 agtgacgcaa agctataggg actaagactt atgaaatgag ttatgtcagc cagttgccaa
     3661 agagatattt tctttttgtt tttatgaatg ttttttaggg ggatatatga tatatgttta
     3721 tggataaaat ggatagatgt acacatatac taactgcata tatatgcagt tcacgcgatt
     3781 actgcaattt tatagataca caattaaatg attttatact agaatatgga gagaacgtgg
     3841 tagaatcttg tttgcatcaa gtgatggtat tggtaagcag atataattaa agaaatttta
     3901 ttttctagaa aacccttttt agttttatct acgttttata attcattaaa gaactaatta
     3961 aaataagctt cattatctat tttcatgaag taaacagttg agtttatctc atatactgtt
     4021 taccccaatt acataattct aacaatatag attccaatga tttaccacgt ttagtagaca
     4081 aatattcaac ttttggagga atagtaggat aagaaatcct cttaataagt ccatctcttt
     4141 caagatactt taattcttgt gacagtatct tatgacttat atgaacaact tcttttttta
     4201 attgactgta acgtattggc tcttgtttta aaatatgcca taagataatt gccctccatt
     4261 tacttcctat cacagtcatg gtaaaaccaa ttgaacaatt aaaatctttt tgtctttctt
     4321 caatatttgg aaattcttca aaattattat ctatatccat ctcaatcctc tctttcaaaa
     4381 tagtaagtat ctaactaaaa agtgcattct tgtttagcgt actaattcgt aatataatct
     4441 tgattgtagc atagagcctt acataatact acttttttag atgtatatgt ttaaatttta
     4501 atatagatat atgttatttt attaatttta tttaaacaga taataataaa taataattta
     4561 tctcaagttt tctatgtaca taaactaatc taatgtttag aaaggattat ataatatgaa
     4621 taaaaaattt cgtctttatc aagttgactc atttacaaaa gaaaaattta caggtaatcc
     4681 agctggtgta attacaaatg ctgatggctt atctgaatca caaatgcaaa aaatagcaag
     4741 agaattaaat aattcagaaa ctgcttttat tttcaataaa ttggctaaaa atcatcaaga
     4801 aaattatgat gtacatatca gattttttac accaactaat gaggttccta tatgtggtca
     4861 tgctactatt gctgcccatt atgttagagc agtagaaaac caattcaata acaaaacagt
     4921 atttcaaaaa actggtgctg gaatattacc agttaatatt ataaaaaaag ataatgatta
     4981 tgaaataatt atgacacaag gaaaaattga agtatctcca ccattacaac aaacctatca
     5041 aaaaagatta cttaatgcat ttggtatttt aaaggaagat attcgtacag attgtcctat
     5101 tgccatatca tctacaggac attcaaaagt tatgattgga ataaaggaat tatcaacatt
     5161 aaatagttta tctccaaatt tatatgaatt atcgaaaata agtcaagaaa taaaatgtaa
     5221 tggatattat atttttacat tacatgataa aaaaactcta caagttcatg gaagaatgtt
     5281 tgctcctgca attggcattt cagaagaccc tgttacagga aatgctaatg gacctttagg
     5341 tgcgtacctt gtacattata aagtaatacc tatacccaac acagagttta tatttgaagc
     5401 agttcaagga gaagcaataa agcgcaaagg caagatgtta gtaaaagtaa ttatttcaga
     5461 taataagcca gttgaagtaa aaataattgg aaaagctgta attgcattct caacaaacat
     5521 agaaattgaa ttataggttc cttctaagta aatttttgaa ctttttaata ctaatttaat
     5581 aacaaactaa tttatataca atcaaattaa tttttttatt atttgttaat atatatttat
     5641 cattatatta ta
//
LOCUS       DAEPXF010000222         5600 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain HB27
            SAMN13376389-rid12479423.denovo.230, whole genome shotgun sequence.
ACCESSION   DAEPXF010000222 DAEPXF010000000
VERSION     DAEPXF010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13376389
            Sequence Read Archive: SRR10518432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5600)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5600)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001069624.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001069624.1
            Genome Coverage       :: 315x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/15/2021 01:54:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,165
            CDSs (total)                      :: 4,076
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 89
            rRNAs                             :: 10, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 10 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 19 of 69
            Pseudo Genes (incomplete)         :: 44 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 10 of 69
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5600
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13376389-rid12479423.denovo.230"
                     /strain="HB27"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="China: Hebei"
                     /collection_date="2017"
     gene            46..270
                     /locus_tag="KRL67_003483"
     CDS             46..270
                     /locus_tag="KRL67_003483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH1524324.1"
                     /translation="MIIKLTKDNFNFRFKQYEGMFFHAEVVEGMESIRYKITKDKEGT
                     ELKEFGSTHIKENMIYVPQIEAYIDLNSIK"
     gene            297..821
                     /locus_tag="KRL67_003484"
     CDS             297..821
                     /locus_tag="KRL67_003484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBH1524325.1"
                     /translation="MSKPIDITIRPIRREDAQAFNEIRRVHSVMRNTLALMSDRVDKT
                     EAMLASLGENDYMFVAETNNNGEKDVIGFAGLHVNSSPRLRHSAEIGISVAENYQGRG
                     VGKKLMEQLLDIADNWIMLARVGLEVIVDNEKGLNLYKKLGFEIEGTKKYAVIRDGKF
                     EDVYIMGRYNKNLI"
     gene            complement(926..1072)
                     /locus_tag="KRL67_003485"
     CDS             complement(926..1072)
                     /locus_tag="KRL67_003485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH1524326.1"
                     /translation="MDMDLLFKIVFAIVAIGVSVKIIKTVASLAFKIALILLIILFVY
                     RMFL"
     gene            complement(1211..2137)
                     /locus_tag="KRL67_003486"
     CDS             complement(1211..2137)
                     /locus_tag="KRL67_003486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HBH1524327.1"
                     /translation="MNFSNIFIQVAVLFIIILVGYFVRKFNLLDDHCTSKLSTLTMTV
                     FLPSMIISSMQINFDSKMIQKILLLLFTSLIMYIVSIIIALLLKYILKCNDKKDLGIY
                     QYIVVFSNVAFMGYPVIEAVLGHEAIFYTAIFNLPFNLFSFTLGIYLLSKGSTSRGFS
                     IKSLISPATIAIVIGLFLFVTGLRLPQFINEPLEMLGSITTPISMIIIGSLLANSSAL
                     DCFVNKKLYIVTFIRLLVLPVIVYFILKGWINDKMILAIPVVISSMPAAANTAIMANQ
                     YDSNITLASQCVFFTTLFSVISIPFISILLLS"
     gene            2327..4198
                     /locus_tag="KRL67_003487"
     CDS             2327..4198
                     /locus_tag="KRL67_003487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sel1 repeat family protein"
                     /protein_id="HBH1524328.1"
                     /translation="MKNTKFSIKKIKNISNDPKVVHTKGALLEKMGKMESAIELYKSA
                     ALDNYVKSQYTLGNIYESKKQFKEAEKWYSMAYKSGSEDAAFDLGNMYYKLDGYEYAI
                     YWYEKIANLDYLQAQNNLGVCHFKMKDFVRCEKWLKTAADKNLGKACFNLGVLYTFLE
                     KDDEAYEYYKKGSVLLDDDAKYNLAILNGKKKDNKSTVSLYKQLYKSGHMKGCFNLGM
                     IMEINDNLEDAEKYYKKSADRDDVKSEYRLAYVYDRKEELGDAIYYYEKAIEKNHTLS
                     KYRLANLYNRENEIEKAKTYYKMAAEDDITEAKNNLAGIYFEEKDYENAIKYYEDAIA
                     VGCKSSLENLGDLYYQNQDIEKAISYYSRIPNNVSCQIKLGNIYEDLNNIEEAISWYK
                     KASENGDTRSSYRLGCIYESLGNTKNARKYFEMASSKNHMNARIHLGRIYFREGKLEE
                     SKMMFDTPANENNVYAQHMVGLIYDMFYKDYVNSKFWYEKARAQGCVESIYNLGQIYL
                     KLNDDAEAEKYYKEGIKYGSKKCEYMLAGLYYKKSLDMYISLANEEYDNCEEIVEGFP
                     NLNIDFDEVLIPPFKLQEEVMDEEEYVPIYILNIKESLDSMLEGLETEMIIDEEHDS"
     gene            complement(4290..5225)
                     /locus_tag="KRL67_003488"
     CDS             complement(4290..5225)
                     /locus_tag="KRL67_003488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HBH1524329.1"
                     /translation="MIRYYKTIDSKLEKLSFFEDGCWINLVEPNHSEINEISNLLNID
                     VESIESALDEEERSRIDVEDNHTLILIDIPVDESDSNSSHYTTIPLGIILTEEAIVTV
                     CDAQTKILNDFIVGHIKDFFTFKKTRFLLQILHKNAAYYLHYLRKINKMTIIIEREIY
                     KSMKNKELVQLLELEKSLVYFSTSLKSNELVLNKMVRTAGIKKYPDDEDLLEDVIVEN
                     RQALDMAKIYGDILSRIMDAFSAIISNNQNNVMQILTVVTLIFSIPTIISGFFGMNVI
                     NMPFSNNPNGFWIILLITSIICIVITFFMSRNKLL"
BASE COUNT         2250 a          592 c          891 g         1867 t
ORIGIN      
        1 ctaataataa gaaaaatatt aattatataa ggagtagata aaaatatgat aataaaactt
       61 actaaagaca attttaattt tagatttaaa caatatgaag gtatgttttt tcatgctgaa
      121 gtagttgaag gtatggagtc cattagatat aaaataacta aagataaaga aggtacagaa
      181 ttaaaagaat ttggaagtac acatataaaa gagaatatga tatatgtacc tcagatagaa
      241 gcatacatag atttgaacag tataaaataa agattatgtt tgagggggaa tccaatatga
      301 gtaagcctat agatataaca ataagaccta taagaagaga agatgcacaa gcttttaatg
      361 aaattagaag agtacacagt gttatgagaa atactttggc attaatgagt gacagagtcg
      421 ataaaactga agctatgtta gcttctttgg gagaaaatga ttatatgttt gtagctgaaa
      481 ctaataataa tggagaaaaa gatgtaatag gctttgctgg gttacatgta aattcttctc
      541 cacgattgag acatagtgca gaaataggaa taagtgttgc agagaattat cagggaagag
      601 gagtaggtaa aaagcttatg gaacaacttt tagacatagc agataattgg attatgttag
      661 ctagagtagg attagaagta attgtggata atgaaaaggg attaaattta tataaaaaac
      721 taggttttga aatagaggga actaaaaaat atgctgtaat cagagatgga aaatttgaag
      781 atgtttatat tatgggaaga tataataaaa atttaattta aaaaagctaa tagagaagta
      841 aaatagagtg tctttaataa aaaatttaca ttaaagataa tctattttac ttctctattt
      901 tattaaaaaa cttattttta aatgtttaca aaaacattct atatacaaat aaaattatta
      961 gcaatatcaa cgctatttta aaagctaaac tagcaacagt tttaatgatt tttacagaaa
     1021 cacctatagc aactatagca aatactattt taaaaagtaa atccatatcc atatttaagc
     1081 ctcctattct ataatacata tagatattat tgacaaatat ctaggaaaaa attccatcaa
     1141 tatactacaa taaaatgttt atatttgtaa tttattgaat atgttattat ataatacata
     1201 aaaaaataaa tcaagataag agtaaaatac ttataaaagg tatagatata actgaaaata
     1261 gtgttgtaaa aaacacacat tgtgaagcta gtgttatatt ggagtcatat tgattagcca
     1321 ttatagcagt atttgcagca gcaggcattg aagaaattac tactggtata gctaaaatca
     1381 tcttatcatt tatccatcct tttagaataa aatatacaat cacaggaagt accaataatc
     1441 ttataaaagt gactatatat aattttttgt tgacaaaaca atcgagagct gaagaattag
     1501 caagtaaact tccaataata atcatggata ttggtgtagt tatacttccc aacatctcaa
     1561 gtggctcgtt tataaattgg ggtaacctaa gtcctgttac aaataaaaaa agtccaataa
     1621 caatagctat tgtagcaggg cttataagcg attttattga aaaacctcta cttgtactgc
     1681 ctttagataa aagataaatt cctaaagtga aagaaaataa gttaaaaggc aaattgaata
     1741 ttgcagtata gaatattgct tcatgaccta aaactgcttc aattacagga taacccataa
     1801 aagcaacatt tgaaaaaaca actatatact gatatattcc caaatctttt ttatcattac
     1861 attttaaaat gtattttaat aaaagtgcaa ttataattga aactatatac ataatcaaag
     1921 atgtaaataa taacaatagt attttttgta tcattttact atcaaagtta atttgcatgg
     1981 agctaattat cattgatggt aaaaaaactg tcattgtcaa agttgaaagc ttagatgtgc
     2041 agtggtcatc taataagttg aattttctga caaaatatcc aactaaaata attataaata
     2101 aaactgcaac ttggataaaa atattgctga aattcataaa gtgtcccccc tatatataca
     2161 ttatatacca attacataat aaaatctaat gattataaaa tttaaaattt aatgttacaa
     2221 tatagtgaca aaaggtaaaa attcaatatt atagcattaa ttgtggatta taatgtataa
     2281 taatgtttga gagaatatat tttataaaat gggtaggtag cgaataatga aaaatacaaa
     2341 attttctata aaaaaaataa aaaatatatc taatgacccg aaagtcgtac atactaaggg
     2401 tgccctcctt gaaaagatgg gtaagatgga aagtgcaata gagttgtata agagtgctgc
     2461 attggataat tacgtaaaat ctcaatatac tttaggaaat atatatgaaa gtaaaaaaca
     2521 atttaaagaa gcagaaaagt ggtattcaat ggcatataaa agtggaagtg aagatgctgc
     2581 ttttgattta ggtaatatgt actataagtt agatggctat gaatatgcta tatattggta
     2641 tgaaaagata gcaaatttag attatttaca agcacaaaat aatttaggtg tatgccattt
     2701 taaaatgaaa gattttgtaa ggtgtgaaaa atggttaaaa acagcagcag ataagaattt
     2761 aggtaaagct tgttttaatt taggggtatt atatactttt ttggagaaag atgatgaagc
     2821 atacgaatac tataaaaaag gttctgtgct tttagatgat gatgcaaaat ataatttagc
     2881 aatattaaat ggaaaaaaga aagataataa atcaacagta agtttatata aacagttgta
     2941 caagtcaggt catatgaaag gttgctttaa cttgggtatg attatggaaa taaatgataa
     3001 tttagaagat gcagagaagt attataaaaa gagtgctgat agagatgatg tgaagtctga
     3061 gtataggctt gcatatgtat atgatagaaa agaagagctg ggggacgcaa tttattacta
     3121 tgagaaagca atagaaaaaa atcatacact ttctaagtat agacttgcta atttatataa
     3181 tagagaaaat gagatagaga aggcaaaaac ttattataaa atggctgctg aagatgatat
     3241 aacagaagca aaaaataatc tggcaggtat atattttgaa gaaaaagatt atgaaaatgc
     3301 tattaaatat tatgaggatg ctatagctgt tggatgcaaa agctcccttg aaaatcttgg
     3361 ggatttatat tatcagaatc aagatataga aaaagcgatt tcgtattatt cgaggattcc
     3421 aaataatgta tcttgtcaaa tcaaattagg aaatatctat gaagatttaa ataatattga
     3481 agaagctata agttggtata agaaagcatc tgaaaatgga gatacgaggt ctagttatag
     3541 attgggatgt atatatgaaa gcttaggaaa tacaaaaaat gctagaaagt attttgaaat
     3601 ggcaagtagc aaaaatcata tgaatgctcg tatacatcta ggtagaattt attttagaga
     3661 aggtaaattg gaagagtcta aaatgatgtt tgatacacct gccaatgaaa ataatgtgta
     3721 tgctcaacat atggtaggat taatttatga tatgttctat aaagattatg taaattctaa
     3781 attttggtat gaaaaagcta gagcacaagg ttgtgttgaa tcaatatata atttaggtca
     3841 aatttattta aaattaaatg atgacgcaga agctgagaaa tattataaag aaggtattaa
     3901 atatggaagt aagaagtgtg agtatatgct tgcaggtctt tattataaaa agagcctaga
     3961 tatgtatatt tctttagcaa atgaagagta cgataattgt gaagagatag tagaaggttt
     4021 tcctaattta aatatagact ttgatgaagt attaattcct ccatttaagt tgcaagaaga
     4081 ggtaatggat gaagaagagt acgtaccaat ttatatacta aatataaaag aaagtttaga
     4141 ttcaatgcta gaagggcttg aaactgaaat gattatagat gaagaacatg atagttaaaa
     4201 agtattataa gattatatag atttaaatac ataaaagcca ttaggataaa tgaaaataaa
     4261 ttaattctaa tggctttttt attaatattt tataatagct tatttcttga catgaaaaat
     4321 gtaatcacta tacatattat agaggtaata agaagtatta tccaaaatcc gtttgggtta
     4381 ttagaaaatg gcatgttgat gacattcata ccaaagaatc ctgatattat tgtaggtatt
     4441 gagaaaatta gtgtaactac tgttaaaatt tgcataacat tattttgatt attacttata
     4501 attgcactga aagcatccat aattctactc aatatatctc cgtatatttt agccatatca
     4561 agagcctgtc tattctcaac tataacatct tctaataggt cttcatcatc tggatatttc
     4621 tttataccag cagttctaac cattttgttt aaaacaagtt cattagattt tagtgaggta
     4681 gagaagtata ccagagattt ttctaattcc aataattgaa ccaattcttt gtttttcatt
     4741 gatttgtaaa tttctcgctc tattatgatt gtcattttat ttatttttct taagtaatgc
     4801 aagtaataag ctgcattttt gtgaagaatt tgaagcaaaa agcgagtctt tttaaacgta
     4861 aaaaaatctt taatatgacc aactataaag tcatttaaaa tcttagtttg agcatcacaa
     4921 actgttacaa tagcttcttc agttagtata atacctaagg gtattgtagt ataatgagaa
     4981 gaattagagt cactttcgtc tactggtata tctataagta taagagtatg attgtcttca
     5041 acatctattc ttgagcgttc ttcttcatct agtgcagatt ctatgctttc tacatctata
     5101 tttaataaat tagaaatttc gttgatttca ctatgattag gttcaacaag atttatccaa
     5161 cagccatctt caaaaaaact aagtttctct aattttgaat ctatagtttt gtaatatctt
     5221 atcatggcaa cacatccttt attcagtttt atataaatct catcatttaa tagtatctat
     5281 taattaacta atttatataa ctgaatatat gtattagttt tgattttaga tattattaag
     5341 tgatgaaaat gtaaaaaatt ctctccgcaa cggactagta tccattgccc atcactctcc
     5401 ttatttatta ttttatatat agctatttat gaaatgcgta ttttaagcac tctttaatat
     5461 aatacctttt ccaggtatat aaatcaagtt ttgatttgat taaataatat ttttaccata
     5521 atttacacat aaagtatata agttatattt aatttgagta aagaatatat atattctgag
     5581 tgaaattatt aaatatatta
//
LOCUS       DAEPXF010000223         5546 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain HB27
            SAMN13376389-rid12479423.denovo.231, whole genome shotgun sequence.
ACCESSION   DAEPXF010000223 DAEPXF010000000
VERSION     DAEPXF010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13376389
            Sequence Read Archive: SRR10518432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5546)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5546)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001069624.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001069624.1
            Genome Coverage       :: 315x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/15/2021 01:54:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,165
            CDSs (total)                      :: 4,076
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 89
            rRNAs                             :: 10, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 10 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 19 of 69
            Pseudo Genes (incomplete)         :: 44 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 10 of 69
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5546
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13376389-rid12479423.denovo.231"
                     /strain="HB27"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="China: Hebei"
                     /collection_date="2017"
     gene            29..145
                     /gene="rrf"
                     /locus_tag="KRL67_003489"
     rRNA            29..145
                     /gene="rrf"
                     /locus_tag="KRL67_003489"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            complement(362..1546)
                     /locus_tag="KRL67_003490"
     CDS             complement(362..1546)
                     /locus_tag="KRL67_003490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HBH1524330.1"
                     /translation="MLSKRLNFITPSYTIGISSKVKEMENNGVKVINLSIGEPDFNVP
                     NNAKSYGIDSLNKDYTKYDLVPGLKILREEICKKLIEENNCNYSIDEIVVSSGAKNSI
                     TNTLLALTDEGDEVLLPKPYWVSYPEMVKLVNAVPVFIDTKKENGFKLTKEELEKSIT
                     DKTKILVINNPSNPTGSVYTKDELIEIVDVCIQNKIYILADEIYEKICYIGEFTSIAS
                     LSEEAKDITITINGFSKSAAMTGLRLGYTASNKTIAKAMSSIQGHLISHPSLTAQYIA
                     YGALKDCSIDIDNMVKTYKSRRDLIKSKLDSIENVGYVNPNGAFYTFIDLSKVSEKFE
                     YKDSFSIEFCNQFLEEYNVAVVPGIAFGMDKYIRISYACSENTFLSGLDKLKEFVYKI
                     MA"
     gene            1919..4270
                     /locus_tag="KRL67_003491"
     CDS             1919..4270
                     /locus_tag="KRL67_003491"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside triphosphate reductase"
                     /protein_id="HBH1524331.1"
                     /translation="MVEFVKKRDGRVIPFNEDRITRAIFLAATNVAEREGIVPDYKLS
                     EQLTQEVIKFLNHKYSESVPSVEDIQDSVVKVLIETGHAKTSEEYIIYRTERSRIRNS
                     KTRLMKAIEEITFEDAEDADIKRENANINGNTAMGTMLQYGSTVSKEFCKTHILKPEH
                     SFAHDNGDIHIHDMDFLNMGTLTCCQIDVKKLFNGGFSTGHGFLREPQDIISYGALAA
                     IAIQSNQNDQHGGQSIPFFDYGLAEGVYKTFKKFYIGNLAKALKLFKGIENNDVIKNI
                     VYNTEKETNQKVGLKRDELYLNLEKEKLIQTFDIDEELVNKMQNFAFEESYRETDKKT
                     YQSMEAFIHNLNTMHSRAGAQVPFSSVNFGTDTSEEGRMVTKNLLLSQERGLGNGETP
                     IFPILIFKVKEGINLNPEDPNYDLFKLSCRVSAKRLFPNFSFLDAPFNAKYYKKGEPD
                     TEATYMGCRTRVLSNVCGSETVSGRGNISFTTVNLPRLGIKHGIINNEKANLDGFFEE
                     LDEKINLIIEQLLERLEVQGNKKMKNFPFLMGQGVWKGSDDLGPEDTLKEVIKQGTLT
                     IGFIGLAECLIALIGKHHGESKEAQELGLKIVSHMRYKMDEATDKYKLNFSLMGTPAE
                     GLSGRFTKIDKKVYGEIKGITDKEYYTNSFHVPVYYNISAYDKIEIEAPYHELTNAGH
                     ITYVELDGDPSDNLEAFETVIKAMKDLGIGYGSINHPVDRDPICGFSGVITSNICPVC
                     GRNEDESDIKFERIRRITGYLVGTVDRFNNAKKAEVRDRVKHR"
     gene            4292..4831
                     /gene="nrdG"
                     /locus_tag="KRL67_003492"
     CDS             4292..4831
                     /gene="nrdG"
                     /locus_tag="KRL67_003492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HBH1524332.1"
                     /translation="MKIRMSSTISYDSIVDGPGLRMVIWTQGCIHNCKECHNPQTHDL
                     CGGFYMDTEEIINKVKSLKLQKGITLSGGEPFLQPEPLEEIAREAKINGLDVWSYTGF
                     TFEQLLDKKNRAYFKNLNLLKQIDILVDGKFVTEKKDISLKFRGSSNQRIIDVQKSLK
                     YKKVFLVEQYMKDDLSIAE"
BASE COUNT         2157 a          625 c          892 g         1872 t
ORIGIN      
        1 ttaatagaaa tattaagcat aaagattatg tggttacaat agcagagagg atacacctgt
       61 tcccattccg aacacagaag ttaagctctc tagcgctgat ggtacttggt gggaaactgc
      121 ctgggagagt aggacgtagc cacgtaattt ttttgttata ataaatttag aatttaactt
      181 atatagttta atttgataat ttgatgaaaa aaataaaaga cttactgatt tttaaatcta
      241 taagtctttt attttttatc taacatttaa attaagttgt tgaatatatc ttatattact
      301 tactttatat attaattatc attaatacat tatatctaat tactattcat ctaaattgtt
      361 attatgccat tattttatat acaaattctt ttagtttatc taatccagat aagaaagtat
      421 tttcactgca agcataagat atacgaatat atttatccat accaaatgct atgccaggaa
      481 caactgcaac attatattct tctaaaaatt gattacaaaa ttctatagaa aaactatctt
      541 tatattcaaa tttctcagat actttagaaa gatctataaa tgtatagaat gcgccatttg
      601 gattcacata tcctacattc tcaatagagt ctagttttga tttaattaaa tctcttcttg
      661 atttatatgt tttaaccata ttgtctatgt caattgagca atctttcaag gctccatagg
      721 ctatgtattg tgctgttaaa cttgggtgag atataagatg tccttgtata gaactcattg
      781 ctttagcaat cgttttatta gatgcagtat atcctaatct taatccagtc attgcagccg
      841 acttagaaaa accatttata gttattgtta tgtctttagc ttcttcgctt aatgatgcaa
      901 ttgatgtaaa ttcaccaatg tagcatattt tttcatatat ttcatctgct aaaatatata
      961 ttttattttg tatgcataca tctactattt ctattaattc atcttttgta taaacactac
     1021 cagtaggatt tgaaggattg tttattacaa gaatttttgt tttatctgtt atagattttt
     1081 caagttcttc ttttgttaac ttaaatccat tttctttttt agtatcaata aaaactggaa
     1141 cagcattaac taacttaacc atttcaggat aactaaccca ataaggcttt ggtaataaga
     1201 cttcatctcc ttcgtctgtt aaagctagta aagtatttgt tattgagttt tttgctccac
     1261 ttgatacaac tatttcgtct attgaatagt tacaattatt ttcttcaata agttttttac
     1321 atatttcctc tctaagtatt tttaatcctg gaactaaatc atatttagtg taatctttat
     1381 ttaaagaatc aataccataa gattttgcgt tgttaggtac attaaaatcg ggttctccta
     1441 tactaagatt tattacttta actccattat tttccatttc tttaacttta gaactaatac
     1501 ctattgtgta ggatggtgtt ataaagttta gtctttttga taacataaaa tgcctccttt
     1561 taaattatta tttatattat acgacaaatt taaagaattt acataaggaa atttgtagat
     1621 atatacatta atatatataa tatcctacac tttttgccta aaattagtgt aaaaaaaaga
     1681 gaaaaaaatt ttgactttca aagttgttag aaacgctact gttcaaaata tccacatagt
     1741 atttgcaatg atttgcgcaa gtttgactat tatgtacaaa tttggtaatc ttaatatagc
     1801 aattattaag ataatccctt taacttagtt gaaggggatt tttttagact taacatacta
     1861 catatagtgg tatggggatt aataaaatac aatatatagt aacaggaggt ttttttaatt
     1921 ggttgaattc gtaaaaaaaa gagatggaag agtaattcca tttaatgaag atagaataac
     1981 aagagctata tttttagcag caacgaatgt tgcagaaaga gaaggaatag tacctgatta
     2041 taaattatct gagcaattaa ctcaagaagt aataaagttt ttaaatcata aatactcaga
     2101 atcagttcca agtgttgaag atatacaaga ttctgtagtt aaggttctta tagaaactgg
     2161 ccatgcaaaa actagtgaag aatatataat atacagaact gaaagaagta gaataagaaa
     2221 ttcaaaaaca agattgatga aggcaataga ggaaattacc tttgaagatg cagaagatgc
     2281 tgatataaaa agagaaaatg caaatattaa tggtaataca gcaatgggaa ctatgttaca
     2341 atatggaagc actgtatcta aagaattttg taaaacccat atattaaaac cagaacattc
     2401 ttttgctcat gataatggag atatacacat acatgatatg gattttttaa atatgggaac
     2461 attaacttgt tgtcaaatag atgttaaaaa actattcaat ggtggatttt caacaggaca
     2521 tggtttttta agagagccac aagatataat aagttatgga gcattagctg caattgctat
     2581 acaaagcaat caaaacgatc aacatggagg tcaaagtata ccatttttcg attatggatt
     2641 ggctgaaggt gtatataaga catttaagaa attctacata ggaaatttag ctaaagcgtt
     2701 gaagttattt aaaggaattg aaaataatga tgtgataaag aacatagtat ataatacaga
     2761 gaaagaaaca aatcaaaaag ttggtcttaa gagagatgaa ttgtatctaa acttagaaaa
     2821 agagaaatta atacagactt ttgatataga tgaggaactt gtaaataaga tgcaaaactt
     2881 tgcatttgaa gaatcgtata gagaaactga taaaaaaaca tatcaatcta tggaagcatt
     2941 tatacacaat ctaaacacaa tgcactctag agcaggtgca caggtaccat tttcaagtgt
     3001 aaacttcgga acagatactt ctgaagaagg aagaatggta actaagaatc ttctattatc
     3061 tcaggaaaga ggtttaggta atggagagac accaattttt ccaattttaa tatttaaggt
     3121 caaagaaggc ataaatttaa atcctgaaga ccctaattat gatttattta aattatcatg
     3181 cagagtatca gctaaaagac tattccctaa ttttagtttt ttagatgcgc cttttaatgc
     3241 aaaatattat aaaaaaggtg aaccagatac agaagctaca tatatgggtt gtagaacaag
     3301 agtacttagt aatgtgtgtg gttcagaaac cgttagtggt agaggaaaca tatcttttac
     3361 tacagtaaat ttaccaagat tgggtataaa acatggtatt ataaataatg aaaaggctaa
     3421 tttagatggt ttctttgaag aattagatga gaagataaac ttaattatag aacaattatt
     3481 ggaacgtctt gaggttcaag gaaataaaaa gatgaagaat tttccattct taatggggca
     3541 aggtgtatgg aaaggttctg atgatttagg accagaagat acactaaaag aagtaataaa
     3601 acaaggaaca ttgacaattg gatttatagg tttagcagag tgccttattg ctcttatagg
     3661 taaacatcat ggagaaagta aagaagctca agaattagga ttaaaaatag tgtcacatat
     3721 gagatataaa atggatgaag caacggacaa atataaatta aatttctcct taatgggtac
     3781 tccagcagaa ggtctttcag gtaggtttac taaaatagat aaaaaagttt atggagaaat
     3841 taaaggtatc acagataaag aatattatac taattcattc catgtaccag tttattacaa
     3901 tataagtgca tatgataaaa ttgagataga agctccatac catgaattga ctaatgcagg
     3961 tcatataaca tatgtagagt tagatggaga tccatctgat aatttagaag cttttgaaac
     4021 tgttataaaa gctatgaaag atttgggtat aggatatgga agtataaatc atcctgtaga
     4081 tagagaccca atatgcggtt tttcaggtgt aataacaagt aatatatgcc ctgtatgtgg
     4141 aagaaatgaa gatgaaagtg acatcaaatt cgaaagaata agaagaataa caggatactt
     4201 agttggtaca gttgatagat ttaataatgc taaaaaagca gaagtaagag atagagtaaa
     4261 acatagatag ttttatttgg gggaataagt tatgaaaata agaatgtcat ctactataag
     4321 ttatgacagc atagttgatg gaccaggatt aagaatggta atatggactc aaggatgtat
     4381 tcataattgt aaagagtgcc ataatcctca gacacatgat ttatgtggag gattttatat
     4441 ggatacagaa gaaattataa ataaagtcaa atcattgaaa ttacaaaaag gcattacgct
     4501 atcaggagga gaaccatttt tacaaccaga accattagaa gaaatagcaa gagaagctaa
     4561 aataaatggg ttagatgtat ggtcatatac tggatttacg tttgaacaac tgttagataa
     4621 aaaaaataga gcgtatttta aaaatttaaa tctactaaaa caaattgata ttttagtgga
     4681 tggaaaattt gtaactgaaa aaaaagatat aagcttaaag tttaggggtt catcaaatca
     4741 aaggataatt gatgttcaaa aaagtttgaa atataaaaaa gtatttttgg ttgaacaata
     4801 tatgaaggat gatttatcta tagcagaata aaatataaat aaacaggatt aaatatatac
     4861 ttatatttaa tcctgtttat tttcatataa tatttcaagc ttgtataatg taacaattaa
     4921 accaaaatat tcattgtata tttgaaatta agttttttat agtttagagg tgttttatgt
     4981 ggattacaaa gaatatagca tccatacgtt atttaatcga ttcttatgcc aagaaacaat
     5041 taatttatag aaattcttaa ttgttacaat ataaatattt agacaagtta taagatactg
     5101 ataaataatt tagaaagttt attaatctaa tattgtgtgt gttgatatta agtggtcaag
     5161 tagtttaaaa aatgaaaaag atttaaaata agtgttttaa ctaaatacat aagtgttaaa
     5221 ttcatgtata agtataggtt agatatataa ggaataatta tgtaatatct atctttgtaa
     5281 aaaaatatta aattttgtaa tttgtggata aatatgttta taatgtggat aaaatttaat
     5341 gtaataagtt aaagcaatat gaatcaactg tttataatgt ggataatatt ataaatgtat
     5401 gaataattgc agtatttaaa aacaaaaaaa gttaaaaaag ttgttgactc taaaagaaaa
     5461 agttggtata gtaatacttg tcctcaagaa aagggcaaac aaataaagca taaagaactt
     5521 tgaaaattaa acagtaggtt aattta
//
LOCUS       DAEPXF010000224         5493 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain HB27
            SAMN13376389-rid12479423.denovo.232, whole genome shotgun sequence.
ACCESSION   DAEPXF010000224 DAEPXF010000000
VERSION     DAEPXF010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13376389
            Sequence Read Archive: SRR10518432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5493)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5493)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001069624.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001069624.1
            Genome Coverage       :: 315x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/15/2021 01:54:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,165
            CDSs (total)                      :: 4,076
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 89
            rRNAs                             :: 10, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 10 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 19 of 69
            Pseudo Genes (incomplete)         :: 44 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 10 of 69
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5493
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13376389-rid12479423.denovo.232"
                     /strain="HB27"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="China: Hebei"
                     /collection_date="2017"
     gene            191..1396
                     /locus_tag="KRL67_003493"
     CDS             191..1396
                     /locus_tag="KRL67_003493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HBH1524333.1"
                     /translation="MSKKVLIMSASTGGGHNRAALAIKEELTSKTLDGEPIECEIIDS
                     LKLVNNTMDKIISRGYEKSAIYTPKAYGSVYRLSETNLLSKNEFKDNLLITFMAKKFK
                     KLIRSEKPDLIIGTHPFPMIALSTLKKNFNLHNNESNAYTEHFYKHYTNTINVPPLIS
                     VLTDYTTHSTWIQNEIDYYIVGHEYVKELLVFDGVEPSKIRTFGIPVEKSFLSHRDKD
                     IVLSELNLSPDKLTVLLMGGSFGAGNIKETLDELLDTDRDFQILVITGKNESLKEKIE
                     KKLMSRYHDKNVCVLGYTDKMNDILASIDVLVSKPGGLTTTEALLKDVPMIVPYYIPG
                     QEEENLDFLSNCGAALRTTKKYNLSILLKVLIDDPSRLEMLKRNIKSIRKSNSAQNIA
                     NLILNIISG"
     gene            complement(1467..1688)
                     /locus_tag="KRL67_003494"
     CDS             complement(1467..1688)
                     /locus_tag="KRL67_003494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH1524334.1"
                     /translation="MKPIMNLEEHNAMKEKRVKKMKSKDKPKYDDFEDISFSNKKKTK
                     KNVRNKNAEFRNNYKNYQYDYEEDFYEPI"
     gene            complement(1830..2429)
                     /gene="recR"
                     /locus_tag="KRL67_003495"
     CDS             complement(1830..2429)
                     /gene="recR"
                     /locus_tag="KRL67_003495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012634696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HBH1524335.1"
                     /translation="MQVYTGPITRLIEEFSKLPGVGRKTAQRLAFHIINMNTNDVEAL
                     SKAIIDAKREIRYCSICCNITDTDPCSMCSNKSRDSSVICVVEDPRDVAAMERTREFK
                     GQYHVLNGVISPMDGIGPDMLKIKELIQRLGNQEVKEIIMATNPTIEGEATAMYIARL
                     VKPMGIKVTRIAHGLPVGGDLEYADEVTISKALEGRREI"
     gene            complement(2442..2792)
                     /locus_tag="KRL67_003496"
     CDS             complement(2442..2792)
                     /locus_tag="KRL67_003496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HBH1524336.1"
                     /translation="MAKKGFGGGMMPGGGNMNNLLKQAQKMQENMQKAQQELESKEVE
                     ASVGGGAVTVKVNGKKEVIDITIKPEVVDPDDIEMLQDLVLSAVNQALRNIDDIQASQ
                     MSKVTGGMNIPGLF"
     gene            complement(2851..4488)
                     /gene="dnaX"
                     /locus_tag="KRL67_003497"
     CDS             complement(2851..4488)
                     /gene="dnaX"
                     /locus_tag="KRL67_003497"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HBH1524337.1"
                     /translation="MHKALYRAYRPQKFEDVIGQDHIIKTLKNQIYSDNIGHAYLFCG
                     TRGTGKTSTAKIFSRAVNCLNKINEEPCNECEICESVLKDNTMDVVEIDAASNNSVDD
                     IRELRESVKYSPANAKYKVYIIDEVHMLSQGAFNALLKTLEEPPSYVIFILATTEPHK
                     IPATILSRCQRYDFKRVTVKDMTLRMKKICEDEGIDIDDKALNLIARNSQGALRDALS
                     ILDQCMSFGESKIDYKDVVELMGSVNIEQLFELSQCIVEQDTKKSLEILNEFVLWGKD
                     IRNLINDLIDHFRNLMVCKVSSELDEIISLPEETIEQLKIQSKNIDINDLIRILNILS
                     ITQDDIKSSSNPRVLVEITIMKIAQPMFDESKEALIKRVENLEKMIELGNFKSEKIGN
                     NKEKKYEVDREIDVKQENVVYEDVKNEDVILIESSWKNILKQIKKDKKMPIYALLSEV
                     KSFNVYSNMLYVIFDDKFDFAKTRLSSQDTINYLEKTIRDVLNRSFNVKIVLTSEVKD
                     INLEVKEKKDIGEEILKNIVSEEILEIKDSIDENESK"
     gene            complement(4565..4829)
                     /gene="ffs"
                     /locus_tag="KRL67_003498"
     ncRNA           complement(4565..4829)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="KRL67_003498"
                     /product="signal recognition particle sRNA large type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01854"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF01854"
     gene            complement(4867..4958)
                     /locus_tag="KRL67_003499"
     tRNA            complement(4867..4958)
                     /locus_tag="KRL67_003499"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4922..4924),aa:Ser,seq:gga)
     gene            complement(4959..5414)
                     /locus_tag="KRL67_003500"
     CDS             complement(4959..5414)
                     /locus_tag="KRL67_003500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HBH1524338.1"
                     /translation="MESSFYMKEALKEAYKAYNKKETPIGAIIVKDNQIIARAHNLTE
                     TLKDSTAHAEILAIKQASEKLGGWRLTDCDLYVTMEPCIMCSGAIVNSRIKKLIIGTR
                     HVKNSYIEKQHEFKLDYFNNNNVKVAFDVLQEECSIILQEFFKALRKRD"
BASE COUNT         1758 a          973 c          624 g         2138 t
ORIGIN      
        1 tacaataata tttttaatat aacatatttg ataaaattaa tttacatttc tttcacttgt
       61 aatttttgca atcttgtatt agattaatat taaaaataat ttatcaataa ccttattcac
      121 aaagacagtt tttatatatc aaaatataat acaatataaa tactatgcta cattttagga
      181 ggattttatt atgagcaaaa aagtattaat aatgtctgct tccactggtg gtgggcataa
      241 tagggctgct ctagctatta aagaagaact tacttccaaa acacttgatg gagagccaat
      301 tgaatgtgaa ataatagata gtttgaaatt agttaataat acaatggata aaataatttc
      361 aagaggatac gaaaaatctg ctatatatac tcccaaagcc tatggaagtg tatatagatt
      421 atctgaaaca aatcttttat caaaaaatga atttaaagat aatctactaa taacattcat
      481 ggctaaaaaa tttaaaaagc ttattcgaag tgaaaaacca gatttgatta taggaacgca
      541 tccttttcca atgattgctc taagtacatt gaaaaaaaac tttaatttac ataataatga
      601 atcaaatgct tacacagaac atttttataa acattataca aataccatta atgttcctcc
      661 tcttatttct gtattaactg attatactac tcattctaca tggatacaaa atgaaattga
      721 ttactatatc gttggtcatg aatatgtaaa agaattattg gtgtttgatg gagttgaacc
      781 gagtaaaatt agaacatttg gaatccctgt tgaaaaatct tttctctctc acagagataa
      841 agatattgtt ctttctgagt taaatttatc tcctgataaa ttaactgttt tacttatggg
      901 gggtagcttt ggggctggaa atataaaaga gacacttgat gaattactgg atactgatag
      961 agactttcaa atacttgtaa ttactggtaa gaatgaaagc ttaaaagaaa aaatcgaaaa
     1021 aaaacttatg tctcgttatc atgataaaaa tgtatgtgtt cttggatata ctgataagat
     1081 gaatgatatt ttagcttcta tagatgtatt agtaagcaaa cctggtggac ttactacaac
     1141 tgaggctctt ttaaaagatg tacctatgat agttccttat tatataccag gtcaagaaga
     1201 agagaatctt gacttcttat ctaattgtgg tgcagcactt agaactacaa aaaaatataa
     1261 cttgtcaata ttactaaaag ttttaataga tgaccctagc agattagaaa tgctaaaaag
     1321 aaatattaaa tcaataagaa agtcaaattc agcccaaaat atagctaatt taattttaaa
     1381 tataatttct ggttgataaa ataaataaaa tccctatagt atatatatta tctattaggg
     1441 attttttatt ttctaattat ttaatattaa attggttcat aaaagtcttc ttcgtaatca
     1501 tactgataat ttttataatt atttctaaat tcagcatttt tatttcgtac atttttttta
     1561 gtttttttct tattagaaaa acttatatcc tcaaaatcat catatttagg tttatctttt
     1621 gatttcattt ttttaactct tttttctttc attgcattat gttcttctaa gttcataatt
     1681 ggtttcatgt tgcaccccta tcaaaatttt taaatgattt atacaacaaa ttatacaata
     1741 cagaagaaat ttattcaata aatctaaatc aaaattaatt tgaatttaac ccaaaaaaat
     1801 tacaaaaatc gaggtattaa cctcgatttt tatatttctc ttctgccttc tagtgctttt
     1861 gatattgtga cctcatctgc atactctaaa tcgcctccaa caggaagacc atgagctatc
     1921 cgagtgactt tgattcccat tggttttact aacctagcaa tatacatggc tgtagcttca
     1981 ccttcaatag taggatttgt tgccattatt atttctttga cttcctgatt gccaagtctt
     2041 tggataagtt cctttatttt taacatatct ggtccaattc catccattgg agaaattact
     2101 ccatttaata catgatactg gcctttaaac tctctcgttc tctccatagc tgctacatct
     2161 cttggatctt ccacaacaca tataacactt gaatctctac ttttatttga acacatgcta
     2221 catgggtctg tatctgttat attacaacaa attgagcaat atcttatttc tctttttgca
     2281 tctataatcg ctttagataa agcttccaca tcattagtat tcatattaat aatatgaaaa
     2341 gccaatcttt gagcagtctt tcttcctact ccaggaagct ttgaaaattc ctctataagt
     2401 ctcgttattg gacctgtata aacttgcata aataccacct actagaataa cccaggtata
     2461 ttcatacctc cagttacctt actcatttga cttgcttgta tatcatcaat atttctcaat
     2521 gcttgattta ctgcacttaa aactaaatct tgtagcattt ctatatcatc tggatcaact
     2581 acttctggtt tgatagttat atctataact tcttttttac cattaacttt tacagtaact
     2641 gctcctccac caactgaagc ttctacttct ttactttcta actcttgttg agctttttgc
     2701 atattttctt gcattttttg agcctgcttt aacaagttgt tcatatttcc accaccaggc
     2761 atcattccgc ctccaaaacc ttttttagcc ataaacacta ttcctcctaa acttattttc
     2821 attttattat tatatcataa tagtatttta ttatttactc tcattttcat caatactatc
     2881 ttttatttct aatatctctt cactcactat gttctttaat atctcttcac caatatcctt
     2941 tttctcttta acttcaagat ttatatcttt aacttctgat gtcagtacta tttttacatt
     3001 aaaacttcta tttaaaacat ctcttattgt tttttctaaa taatttattg tatcttgaga
     3061 gctcagtcta gtttttgcaa aatcaaattt atcatcaaat ataacataca acatattaga
     3121 atatacatta aaacttttta cttcacttaa gagtgcgtat attggcattt ttttatcttt
     3181 tttaatttgt ttaagtatat tcttccaaga tgactctata agtataacgt cctcattttt
     3241 tacatcttca taaactacat tttcttgttt tacatctatt tctctatcga cctcatactt
     3301 tttttcttta ttattcccaa ttttttcaga cttaaagttc cccagttcaa tcattttttc
     3361 taagttctca actcttttta taagagcttc tttactttca tcaaacattg gttgagcaat
     3421 tttcattatg gttatttcca ctaaaaccct tggatttgaa gatgacttta tatcatcctg
     3481 agttattgat aatatattta aaatccttat taaatcattt atatctatat ttttagactg
     3541 aattttaagt tgttctatag tttcttctgg taatgaaatt atttcatcca attctgatga
     3601 aaccttacat accattaaat ttctaaaatg atcaattaaa tcatttataa gatttcttat
     3661 atctttaccc cataagacaa attcatttaa tatctcaaga gactttttag tgtcctgctc
     3721 tacaatacat tgagacaatt caaataattg ttctatattt acgcttccca ttaattcaac
     3781 aacatcttta taatctattt tactttctcc aaaagacata cattggtcta atatactaag
     3841 tgcatctcta agagctcctt gagaatttct agctattaaa ttaagagctt tatcatctat
     3901 atcaattcct tcatcttcac aaatcttttt cattctaagg gtcatatctt taactgtaac
     3961 tcttttaaaa tcgtatcttt ggcatcttga taaaatggtt gcaggtattt tgtgaggctc
     4021 tgtagttgct aatataaaaa ttacatatga tggaggttcc tctaaggtct ttaataatgc
     4081 attaaaagct ccttgagaca acatatgaac ttcatctatt atatatactt tatatttagc
     4141 attggctgga gaatatttca cactttctct aagctctctt atatcatcta cactgttatt
     4201 tgaagctgca tctatttcta caacatccat tgtattatct tttaatacag attcacatat
     4261 ctcacactca ttacacggtt cttcatttat tttattcaaa cagtttactg ctcttgaaaa
     4321 aatctttgca gtagatgttt taccagtacc tcttgttcca caaaacaaat aagcatgtcc
     4381 tatattatca ctatatattt ggttttttaa agtcttaata atatgatctt gaccaataac
     4441 atcttcaaac ttttgtggtc tatatgctct atataatgct ttatgcatct ttttacctct
     4501 ctttagcaaa attaataaaa aaatccctca ttatctaagg gattagtctt ttatatatta
     4561 aatgctgtac accatctatc gacttaattc cataggcgtt accaaaacag ttagctcaac
     4621 ttaggcaccc ccacggcaca caaaagtgat cacttaccgc tgcttccttc cggatctgac
     4681 ggggttcatg agctttcatt gcgcaggacc taagcatcaa caccacttat ttagggcggc
     4741 cctacagaaa ataagccttt agatggaatt caatcctgct atagcggatt gcaggttaca
     4801 gggcaccgct acctccccat ctagtacagc aaaattacaa ccatatttaa ttactttcat
     4861 tgtttctggc ggagagagtg ggatttgaac ccacgataca gttttaccca tatactcgct
     4921 ttccaggcga gcgccttcga ccactcgacc atctctcctt aatctctttt tctcaatgcc
     4981 ttaaaaaatt cttgcaatat gatcgagcat tcttcttgca aaacatcaaa agctactttt
     5041 acattattat tattaaaata atctaattta aactcatgtt gtttttctat atatgaattt
     5101 tttacatgtc ttgtccctat tataagcttt tttattctag aattaactat tgcaccacta
     5161 cacattatgc aaggttccat tgtaacatat aaatcacaat cagtcaacct ccaaccacct
     5221 aatttttctg atgcttgttt tatcgctaaa atttctgcat gtgctgtaga atcttttaac
     5281 gtttctgtaa ggttatgagc tctagctatt atttgattat ctttaactat aatagcacct
     5341 attggtgttt cttttttatt atatgcttta tatgcttctt ttaatgcttc tttcatataa
     5401 aatgaacttt ccataattcc tcccaaaact tataacaaac ttatcttatc ataattcatt
     5461 ttataattca acttaatttt attttgttta ttt
//
LOCUS       DAEPXF010000225         5445 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain HB27
            SAMN13376389-rid12479423.denovo.233, whole genome shotgun sequence.
ACCESSION   DAEPXF010000225 DAEPXF010000000
VERSION     DAEPXF010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13376389
            Sequence Read Archive: SRR10518432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5445)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5445)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001069624.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001069624.1
            Genome Coverage       :: 315x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/15/2021 01:54:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,165
            CDSs (total)                      :: 4,076
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 89
            rRNAs                             :: 10, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 10 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 19 of 69
            Pseudo Genes (incomplete)         :: 44 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 10 of 69
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5445
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13376389-rid12479423.denovo.233"
                     /strain="HB27"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="China: Hebei"
                     /collection_date="2017"
     gene            214..396
                     /locus_tag="KRL67_003501"
     CDS             214..396
                     /locus_tag="KRL67_003501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH1524339.1"
                     /translation="MFKDKIDECVHIMTAYIASLKEYYSFIETQIGDFIKKYGEDIVE
                     LCLHRVMILLCECGLA"
     gene            962..1876
                     /locus_tag="KRL67_003502"
     CDS             962..1876
                     /locus_tag="KRL67_003502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2935 domain-containing protein"
                     /protein_id="HBH1524340.1"
                     /translation="MIDNQKYVILSLELHLFFSRIMKEHALFLEAGFTNKNYNLAMEA
                     DHYKKQFEDLLSYAVSASNGIIRPDILYSEELVTTLTSAAEQKTEEFTGIEINKNITT
                     RELNLQSGVDPQVGQDLVNYVAQLNSDAIRLLDGLINFKERVLDGVLSCTIFTSNYPL
                     LLEHIIHEANLYRSYVVDLENKIDIESKSAKEIELFWDHIMMEHALFMRGLLDPSEGE
                     LINTSNDFAIKFNELIEKTNEMTDSNIKNITEETLNETVEFKDFKEAGASGIEQCKIK
                     SIILPLLADHVLREANHYIRILESYKNM"
     gene            2266..2913
                     /locus_tag="KRL67_003503"
     CDS             2266..2913
                     /locus_tag="KRL67_003503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein"
                     /protein_id="HBH1524341.1"
                     /translation="MIRQTPLEKRYDKLGPHIVTALKKRYFDAYYCKTRNEALQQILD
                     LIPQNHVVSWGGSETLKEMGVQTAVREKGFKVIDRDLAKTPEERTEIMRQALLCDTFL
                     MSSNAISEDGQLFNIDGNGNRVAAMIFGPKNVIVVAGMNKIVKNIDDAIQRTRTIASP
                     AVVQRFQDVNTPCYINGSCIDCTSPESICAYMVTTRISRPAKKIKVILVGENLGL"
     gene            complement(3080..3649)
                     /locus_tag="KRL67_003504"
     CDS             complement(3080..3649)
                     /locus_tag="KRL67_003504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH1524342.1"
                     /translation="MKKFIICIIMFAISVLVLAGCSNDNKIKNEVDSMFNAIKTQDAD
                     TVDKYFPKSGLGNLIYEDKEGFKMYSKNMTWEISDIKNNKDKVTVDLKINNTDMVSIL
                     KKNPPKLGEMMPNPTMKDIDSAKKKEFNITLELVKTGDNYVCKVNQESAMKLLNVITG
                     GGIDYLADYNKDYYKQLDEEQKNYENKNS"
     gene            complement(3654..4553)
                     /locus_tag="KRL67_003505"
     CDS             complement(3654..4553)
                     /locus_tag="KRL67_003505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HBH1524343.1"
                     /translation="MKLLSKINNNIRLITQVAFTALTNGYVNGFLEGTIYGGESKKLC
                     VPGLNCYSCPGALGACPIGSLQAVLSSREYKFSFYVVGFLMAFGAFFGRFVCGWLCPF
                     GLVQDLLHKIPFPVKIKKVYGDKYLKYLKYIVLIMFVIILPVAVVNVAGGGNPWFCKW
                     ICPSGTLLGGIPLILGNENLRESIGFLFSWKLSILLLILLMSIITYRPFCKYICPLGA
                     IYGFFNPISIYRFKIDKDKCTNCTACQTKCKLDIPIYKDVNSSDCIRCGECIKVCPQN
                     AITTTFSKDKKENNICAKKYRGI"
     gene            complement(4706..5290)
                     /locus_tag="KRL67_003506"
     CDS             complement(4706..5290)
                     /locus_tag="KRL67_003506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA family protein disulfide reductase"
                     /protein_id="HBH1524344.1"
                     /translation="MKKFFMVVLGCVFVLGMISGCSKKESKPVEKNKNNSEWFSNLDT
                     EDVDGNKVTKEIFSNKELTLINVWTTWCGPCVGEMPELEALSKEYESNNSNVAIKGLV
                     VEVDKTDMRTGLSNEEKNLVKDIMKKSDATYQQLTVSEELKKTDFKRVMEFPTTYFVD
                     KKGNFVGEKVVGANSKEDWKKIIDERLKMVKTNE"
BASE COUNT         2000 a          818 c          633 g         1994 t
ORIGIN      
        1 tactaactta aatatttata accaaacttt aaactggata aattttcctt taagaatgaa
       61 aatttatcca gttttgcgat tataataacc agttttacat atccacttgt aaaatatatc
      121 taagcaatat atcatactat atagatttat gagtgaaata atatctgcta tttactgaat
      181 atttttattt ttcgtttttt agggggatta gttgtgttta aagataagat tgatgaatgt
      241 gttcatataa tgactgcata tattgctagt ttaaaagaat actattcatt catagaaact
      301 caaattggtg attttattaa aaaatatgga gaagatattg tagagttatg tctccataga
      361 gtgatgattc tactatgcga atgtggtttg gcataaaatt aaatgtatta aaatgggtaa
      421 gaagaatcct atataatatt cttcttaccc attttaatat atcaatttca tagtttatcg
      481 tttaaaatat tttacatcca ttactagaca actttacatt ccatttttaa ttctaattta
      541 tcacttaatt ttttatactt tttaaatcct atacctaaaa cattaagtgc aactattaga
      601 attaataatt gttgtggtat aaaattatgc ttgcttcata tttattcatt ttttcctata
      661 aaagttcttg ttttacaaaa aacatttgtt ttgtatcaaa ataatactta taaattacat
      721 aattctcata aaaatttcta gtatcacttg tactcttaat aaataatagc caacaagcac
      781 cttaaatcat tatatctata tctgataaga aacacagtaa gataattttt tcacccataa
      841 cttaataaaa actgttgata ttagtagcaa ttgaatttag ttaatttatt tataatatac
      901 ataaatttaa atattacgaa taaagtatta taaatatgat attttctaaa gggaggaaat
      961 tatgatagat aatcaaaaat atgttatttt atcactagaa ttacatttat ttttttcaag
     1021 aattatgaaa gagcatgctc tttttttaga agcaggattc acaaataaaa attataatct
     1081 tgctatggaa gctgaccact ataaaaagca atttgaagat ttattatcat acgctgttag
     1141 tgctagtaat ggtataatta gacctgatat attatattca gaagagcttg taactactct
     1201 cacatcagct gcagaacaaa aaacagaaga gtttacaggg atagaaataa acaaaaacat
     1261 cactacaaga gaattaaatc tacaaagtgg tgtagaccca caagttggtc aagacttagt
     1321 gaattatgta gctcaactta actctgatgc aataagatta cttgatgggc ttattaattt
     1381 taaagaaaga gtcttagatg gtgtactatc atgtactata tttacatcaa actaccctct
     1441 acttcttgaa catataatac atgaagcaaa tttatatcgt tcttatgtag ttgaccttga
     1501 aaataaaata gatattgagt caaaaagtgc taaagaaata gaattattct gggaccatat
     1561 tatgatggaa catgctctgt ttatgagagg attactagac ccctcagaag gtgaactaat
     1621 aaatacttca aatgattttg ctataaaatt taatgaatta attgaaaaaa caaacgaaat
     1681 gactgattct aatatcaaga acattacaga agaaactcta aatgaaactg ttgaatttaa
     1741 agattttaaa gaagcaggag catcaggaat agaacagtgt aagataaaat ctataatatt
     1801 accactttta gcagaccatg ttttaagaga ggcaaatcat tatattagaa tattggagag
     1861 ttataaaaac atgtaattaa aaattacttt taaaataatc tttgcatatt tagataaaat
     1921 aaaggtacag ttaactgtac ctttattttt tataaactta taaaatttaa acaagttatt
     1981 cataaaataa aataataatc atctataacc cacaacttta ttttatatta aataaaaagt
     2041 attagtgatt atgttttata ggtatttctt tattcagtta tttattaaaa actaatagta
     2101 aatcttatac taatcgcata tttatgttat aactatattg taactatttt cgtaatctta
     2161 ggaaaagatt acttataaaa ttaaccttat acaataatat atcgtataat cttaattagc
     2221 ataatagtta caatattaaa atttaaatat gggagtgatt ttagtatgat aagacaaaca
     2281 ccattggaaa aacgatatga caagctaggt cctcatattg taacagcact caaaaaacgt
     2341 tattttgatg cttattactg taaaactaga aatgaagctt tgcaacaaat tttagattta
     2401 attcctcaaa atcatgtagt atcatgggga ggatctgaaa cacttaaaga aatgggcgta
     2461 caaactgctg ttagagagaa aggatttaaa gttattgata gagatttagc aaaaactcca
     2521 gaagaaagaa ctgaaattat gcgtcaagca cttctatgcg atactttttt gatgagtagc
     2581 aatgcaatta gtgaagatgg acaactcttt aatattgatg gaaatggcaa tcgtgtagct
     2641 gcaatgatat ttggtccaaa aaatgttata gttgtagctg gtatgaataa aattgtaaaa
     2701 aacattgatg atgcaataca aagaactaga acaattgcat ctccagcagt tgttcagcgt
     2761 tttcaagatg tcaatacacc atgctatatt aatggaagtt gtatagattg tacaagtcct
     2821 gaaagcattt gtgcatacat ggttactaca agaatcagtc gtccagcaaa aaaaataaag
     2881 gtaatattgg taggagaaaa tcttggtcta taaaatatac atacacgtaa atagctctat
     2941 aaataataaa atcatatgaa ataacttatt tctaaaagtg gtagaataaa agcttctaaa
     3001 agactttcat tttaccactt ttatgttctt ttaatttgtg cttctctatt attaattgct
     3061 ccgattttca tatatattac taactatttt tattttcata atttttttgt tcttcatcaa
     3121 gctgtttata ataatcttta ttatagtcag ctagataatc tataccacct cctgtaatca
     3181 catttaataa tttcattgca gattcttgat taactttaca aacataatta tcaccagttt
     3241 taacaagctc taatgtaata ttaaattctt ttttctttgc agaatcaata tctttcatag
     3301 ttggatttgg catcatttct cctagtttag gaggattctt ttttaggata gaaaccatat
     3361 cagtattatt aatttttaaa tctacagtca ctttatcctt attattcttt atatctgaaa
     3421 tttcccaagt catattttta gaatacattt taaatccttc tttatcttcg tatataagat
     3481 tacctaaccc tgattttggg aaatacttat caacagtatc tgcatcttgt gtttttattg
     3541 cattaaacat actatccact tcatttttta tcttattatc attactacaa ccagctaaca
     3601 ccagtactga tattgcaaac attataatac atataataaa tttcttcatt aaattatatc
     3661 cccctatact tttttgcaca aatattattt tcttttttat cttttgaaaa agttgtagta
     3721 attgcatttt gtggacaaac ttttatacat tcaccacacc taatacagtc tgaactattt
     3781 acatctttat atattggtat atcaagctta cattttgttt gacatgctgt gcaattcgta
     3841 catttatctt tatcaatttt aaatctatat attgaaatag gattaaaaaa accatagatt
     3901 gctcctaaag ggcatatata cttgcaaaat ggcctataag taatgattga catcaataat
     3961 atcaatagca aaatacttaa cttccagcta aataaaaatc ctattgattc tcttaaattc
     4021 tcatttccta aaataagtgg tattcctccc aaaagtgttc ctgaagggca aatccattta
     4081 caaaaccaag gatttccacc tcctgctaca tttacaacag caactggcag aataattaca
     4141 aacattatta agactatata ctttagatat tttagatatt tatcaccata aacttttttt
     4201 atctttactg gaaagggtat tttatgtagt aaatcttgta caagtccaaa gggacaaagc
     4261 caaccacaaa caaatctgcc aaaaaaagca ccaaatgcca ttaaaaaacc aacaacataa
     4321 aatgagaatt tatactctct gcttgataaa actgcttgta atgaacctat tggacatgct
     4381 ccaagtgcac ctggacatga ataacaattc aatccaggta cacataattt tttactttct
     4441 cctccatata tagttccttc taaaaatcca tttacatatc catttgttaa tgctgtaaat
     4501 gcaacttgag taataagtct tatattatta tttattttag ataataactt cattcctctt
     4561 ctcctaacca ataccaatac actcaagaca aatcataatt gctttagtca atactgtatt
     4621 tgtttctcca cggtatgtac ctaatacaat aaatataata ctaattacaa taatagaaat
     4681 gcctttttta ttatctttaa taaagttact cattggtttt taccattttt agtctttcat
     4741 ctataatttt tttccaatct tccttactat ttgcacctac aaccttctca cccacaaagt
     4801 ttcctttctt atctacaaaa taagttgttg gaaactccat aactctttta aaatcagttt
     4861 ttttaagttc ttcagataca gtcaattgtt ggtaagttgc atctgacttt ttcataatgt
     4921 ctttaactaa atttttctct tcatttgaaa gaccagttct catatcagtt ttatcaactt
     4981 ctacaactaa tccctttata gctacattgg aattattact ctcatattct tttgataatg
     5041 cttcaagctc tggcatttca cctacacatg gtccacacca agtagtccag acattaataa
     5101 gagtaagttc tttatttgaa aatatctctt ttgttacctt atttccatca acatcctccg
     5161 tatctaaatt actaaaccat tcactattat tcttgttttt ttctactggc ttagattctt
     5221 ttttactaca tccagaaatc atacctaaaa caaaaacaca tcctaaaact accataaaaa
     5281 actttttcat atctcacact cctagaattt atatagtata taaaatcgct gcttgaaata
     5341 aaaataactt ctcttctata ttaaaacatt atatttaata aataagaact aatttaaaat
     5401 aagttgtata aaagatataa agttaaaatc tatttctttt cagac
//
LOCUS       DAEPXF010000226         5395 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain HB27
            SAMN13376389-rid12479423.denovo.234, whole genome shotgun sequence.
ACCESSION   DAEPXF010000226 DAEPXF010000000
VERSION     DAEPXF010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13376389
            Sequence Read Archive: SRR10518432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5395)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5395)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001069624.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001069624.1
            Genome Coverage       :: 315x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/15/2021 01:54:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,165
            CDSs (total)                      :: 4,076
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 89
            rRNAs                             :: 10, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 10 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 19 of 69
            Pseudo Genes (incomplete)         :: 44 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 10 of 69
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5395
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13376389-rid12479423.denovo.234"
                     /strain="HB27"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="China: Hebei"
                     /collection_date="2017"
     gene            complement(529..1935)
                     /locus_tag="KRL67_003507"
     CDS             complement(529..1935)
                     /locus_tag="KRL67_003507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBH1524345.1"
                     /translation="MDSVKYILRRFIGSSILISLLFIVLNIAGFLLFSYMLFVSKYSD
                     NLDTSDSREIKSNLIMIEDNLILKDGKFILDDSSKEVLHKNKVWAMLIDNNGDRVWGF
                     DLPKEIPSHYTLTDVAKFSRFFLKDYPVYVWEHPNGILVTGYPKDKYTKFNLTYTIKE
                     IEQFPMLIIGMFLVNAIVTFLIALFIGLKMVKSIKPIITGIKLMSKGEPVLLQEKGIL
                     SDISKSINTVSKELQMKDEKLRKKEEARSNWIAGISHDIRTPLSMIIGYAGELEESSS
                     LSNREQEQVSIICNQGVKIRELVNDLNLVSKLEYNMQALNCDKIRVSAFLRELVSEFI
                     NNSLDNDFYIELDILNEDIIIDADKKLLKRAISNLIQNSITHNQQGCNIRVTSKSDLD
                     FCYIIVEDDGKGMSQEKINSLLKSQNGLSEAYAKGQNHGLGILIVSGIINAHRGKLDI
                     TSIDSQGLKIILKLPLIK"
     gene            complement(1980..2687)
                     /locus_tag="KRL67_003508"
     CDS             complement(1980..2687)
                     /locus_tag="KRL67_003508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBH1524346.1"
                     /translation="MESIKDKKILVVDDHKELLKMIDEILRKEGFSRVFLASSYEEAV
                     RVFKNVKPDCAILDVVLPDGDGFSIMRKIRETSKIPVIFLSARGEDEDRLIGLGLGAD
                     DYIVKPFLPKELTLRLVGILNRVYVPIEEEELPVFKLGDSVVVNLNSACVEKDGQEIS
                     LTAKEHSLLLKLYENKGRIVTSDSLCQAIWGDDMYGYENTLMVHVRRVREKIEKTPST
                     PRHLLTVRGLGYKLMIN"
     gene            2858..3772
                     /locus_tag="KRL67_003509"
     CDS             2858..3772
                     /locus_tag="KRL67_003509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBH1524347.1"
                     /translation="MEMIIRTQRLSKEYNKIFRVKDIDLRVPKGAVYGFLGPNGAGKS
                     TTLKMLLGLAKPTQGNINILGKELNEKNRISILKDIGSLIESPSYYGHLTGLENMTIM
                     QRLLDLPKKNVNEALKIVRLENQKNKKVSQYSLGMKQRLGIAMAIMKFPKLLILDEPT
                     NGLDPAGIEEIRELIKSLPEKYGMTVLISSHLLSEIDQIATSIGIINHGELVFQDSIK
                     ELHNRGQSQIAIKTKNVVSAQKLLNTKGYMSLIQDGYLTIENLKDSDVAKINGMLVHS
                     NIDVFRIEEHKKSLEDIFLELTGKAVTL"
     gene            3772..4533
                     /locus_tag="KRL67_003510"
     CDS             3772..4533
                     /locus_tag="KRL67_003510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBH1524348.1"
                     /translation="MKYLGLEFYKLKRKKIILMTFLFVAVEIMWCIVNSNRAITRNPD
                     MLGGFEYSYMLMSFASLNGLFFPILISIITSRISDIEHKGDTWKLLKSSVTSLNSIYS
                     SKFLCSAILVSVAVLMQVLSIVLFGHFRGVIESISVFSLLKYTLGTILVSIAIIALHL
                     WIATVIPNQMVAITFGMIGSFIGLTSGLFYQGIRRLFIWSYYLELSPLSYAYDNVLGS
                     SVYRIDMNFSITILVFLIGMLIYYIGRNHLLKKEV"
     gene            4545..5282
                     /locus_tag="KRL67_003511"
     CDS             4545..5282
                     /locus_tag="KRL67_003511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBH1524349.1"
                     /translation="MLRKSVLGEILKLKNSFIWYVLLALPLISVLIGSGNFYLNQGIL
                     KKEWYSLWTQVSLFYGEFFFPILISIFCAYICRLEHMNHNWNNVMTLPVKLKNIFLSK
                     LTVVSILTAITQVFFVIFYIVAGNMFGFSGSVPKELTGWIAKGWIISISIASIQLYMS
                     IKIRSFATPIGISLCLSLFGMGLIVAKPAVGIFFPYSMLGSAMGVIKQSALNVNDTIV
                     TICVAFITTILFSEIANRNFRKNDVIS"
BASE COUNT         1876 a          837 c          699 g         1983 t
ORIGIN      
        1 aaaaatacta cacaaaaact ctactttagt agaactcaag gggggcaaaa tacttcaatt
       61 taaaacattt atcgtacttt aaataaataa ctataataaa atatacttac aatagttaca
      121 tagaagctat tataactaca cagaacaacg tataattaaa acttttttag gatgtcatta
      181 tattttttct taaaattaaa aaacactata ccataattct attttagtag aatttttttg
      241 tcaataataa aattaaatat taactaaaaa ataaaaaaaa tggaattatc taaagaattt
      301 aataacctta caataattcc aaaatattat ttctatgaaa attttgatac catgagtaac
      361 tcagccataa gcgcataaag cacaagttac tctttacttt aataaaactt gacaagaaaa
      421 gaatttatca ttattctact ataatagagt taatttgtca ataaagaata aagttatttt
      481 caaaatattt tcattcttca ttcttatctg ttattatttt ataaaatatc acttaattag
      541 aggtaatttt agtattattt ttagtccctg actatcaata ctcgtaatat ctaatttacc
      601 tctatgtgca ttaataatgc cagatactat caaaatacct agaccatgat tttgaccttt
      661 ggcatatgcc tcacttagtc cattttggct ctttaataaa ctattaattt tttcttgact
      721 catacccttt ccatcatctt caacaattat atagcaaaaa tctaaatcag acttagaggt
      781 aacccttata ttacatcctt gttggttatg agttatacta ttttgtatta aattactaat
      841 agcccttttt aataattttt tatctgcatc tataataata tcttcattta gtatatcaag
      901 ttcaatataa aagtcattat ccaaactatt atttataaac tcagaaacaa gctctcttaa
      961 aaacgcagaa actctaatct tatcacaatt caatgcttgc atattatatt caagtttaga
     1021 cactaaattg agatcattta ctaattctct gattttgacc ccttgattgc atataataga
     1081 tacttgctct tgttctctat ttgaaagact actgctttct tcaagttctc cagcatatcc
     1141 aattatcatt gaaagtggag ttcttatatc atgagatatt cctgcaatcc agttagacct
     1201 agcttcctct ttcttcctaa gtttttcatc tttcatttgc aactctttag atacagtatt
     1261 aatagatttt gaaatatcac ttagtattcc tttttcttgt aaaagtactg gttccccttt
     1321 tgacataagc tttataccag taataattgg ttttatagat ttcaccattt ttaaacctat
     1381 gaatagagct attaaaaaag taactattgc gttaaccaga aacataccta ttattaacat
     1441 aggaaattgt tctatttcct taatcgtata agtaaggtta aacttagtat acttatcctt
     1501 tggatagcct gttactaata ttccatttgg atgttcccat acatacacag gataatcctt
     1561 taaaaagaac cttgaaaact ttgctacatc tgtcaaagta taatgacttg gaatttcttt
     1621 tggtaaatca aatccccata ctctatctcc attgttatca attaacattg cccaaacttt
     1681 atttttatgt agtacttcct tactagaatc atctaaaata aactttccat cttttaaaat
     1741 caaattatct tcaatcataa tcaagttgga ttttatttct ctgctatcac ttgtatctaa
     1801 attatcagag tatttagata caaatagcat atatgaaaac agaagaaaac ctgctatatt
     1861 taatacaata aaaagtaatg aaatcaagat acttgaacct ataaatcgcc tcaatatgta
     1921 ctttacacta tccaaaatta aatcactcct cttgatttct tttattctaa tatctaaaat
     1981 taattaatca tcaacttata tccaagacct cttactgtaa gaagatgtct aggtgtagat
     2041 ggagtttttt ctatcttctc tctaaccctt ctaacatgaa ccataagggt attttcatat
     2101 ccatacatat catctcccca aatggcttga catagtgaat cactagtaac aattctccct
     2161 ttattttcat acaactttaa aagcaatgaa tgctctttag ctgttaaaga aatctcttgc
     2221 ccatctttct caacacatgc actatttaaa ttcacaacaa cagaatctcc cagtttaaat
     2281 actggcaact cctcttcttc tataggcaca tatacacgat ttaatatacc cacaagtctt
     2341 aaagtaagtt cctttggtaa aaatggcttt actatataat catcagcccc taagcccaaa
     2401 ccaatcaatc tatcttcatc ttctcctctt gcagagagaa aaataactgg tatcttagat
     2461 gtctctctaa tttttctcat aatagagaaa ccatctccat ctggcaatac aacatctaaa
     2521 attgcacaat ctggctttac atttttaaat accctaacag cttcctcata acttgatgct
     2581 aaaaatactc tactaaatcc ctcttttctt aggatttcat ctatcatttt taataattcc
     2641 ttgtggtcat caaccacaag tattttttta tcttttattg actccataat catcaccctc
     2701 ctttagtata ccatataccc attttcacaa ctttgcctaa atagactttt aaggttattg
     2761 taaggtaaac ttcagccaac tgaaatgtaa ccaagttatt atactgatat aagctaaaag
     2821 aatcaataaa cacagattaa aaaaggggat gaaacttatg gaaatgataa ttagaacaca
     2881 aagactttca aaggaataca ataaaatatt tagagtaaag gatatagacc taagagttcc
     2941 aaaaggagct gtgtacggtt ttttaggtcc taatggagct ggaaaatcta ccactcttaa
     3001 aatgcttctt ggacttgcta aacccacaca aggaaatatt aatattttag gtaaagagtt
     3061 aaatgaaaaa aatagaatat caattttaaa agatataggt tctttgatag aatctccatc
     3121 atactatggt catctgacag ggcttgaaaa catgactata atgcaaagac tcttagattt
     3181 gcctaaaaaa aatgtaaatg aagcattaaa aattgtaagg ttagaaaatc aaaaaaataa
     3241 aaaagttagt caatattctt taggtatgaa gcaacgtctt ggcattgcta tggcaataat
     3301 gaaatttccc aaattattaa tacttgatga acctacaaac ggactcgacc cagcagggat
     3361 tgaggaaatt agagaattaa taaaatctct accagaaaaa tacggaatga ctgttctaat
     3421 ctctagccat cttttaagtg aaattgacca aattgccaca tcaataggaa taataaatca
     3481 tggcgaactg gtttttcaag atagcataaa agaacttcat aatagaggtc aaagtcagat
     3541 tgctatcaaa actaagaatg ttgtatctgc acaaaaacta ttaaatacta aaggatatat
     3601 gtcacttata caagatgggt acttgactat tgaaaattta aaagactcag atgtggctaa
     3661 aataaacggt atgcttgtcc attcaaatat agatgtattt agaatagaag aacataaaaa
     3721 aagcttggaa gatatattcc tagaacttac tggtaaggcg gtgactttat aatgaaatac
     3781 cttggtctag aattttataa acttaaaaga aagaagatta ttctgatgac ctttttattt
     3841 gtagctgttg aaataatgtg gtgtatcgta aattcaaata gggctattac acgtaatcca
     3901 gatatgttag gtggatttga atattcctat atgcttatga gttttgcaag tttaaatggt
     3961 ctattctttc ctatattaat atcaataatt acatcacgta taagcgatat agaacataaa
     4021 ggagatacat ggaagctttt aaaatcttct gtaacatctt taaatagtat ttattcatct
     4081 aaatttttat gttctgctat tttggtatct gtagctgtat tgatgcaagt gttatctatt
     4141 gtgttgtttg gacattttag aggggttatt gagtcaattt ctgtattttc acttttaaaa
     4201 tacacattag gaacaattct tgtaagtatt gctatcattg cactacacct atggattgca
     4261 actgtaattc caaatcaaat ggttgcaatt acatttggca tgattggtag ttttataggt
     4321 ttgacatcag ggttgtttta tcaaggtatt cgtagattat ttatatggtc ttattaccta
     4381 gagttatcac ctttaagtta cgcctatgat aatgttttag gttcatctgt ctatagaatt
     4441 gatatgaatt tttctattac gattttagtt ttcttaattg gaatgctaat ttattatatt
     4501 ggaagaaatc atttattaaa aaaagaagta taggagggat ttaaatgtta aggaagtcag
     4561 ttttgggaga aattctaaag ttaaaaaatt cttttatatg gtatgtatta ttagctttgc
     4621 cacttataag tgtattgatt ggttctggta atttttatct aaatcaaggc atactcaaaa
     4681 aagaatggta cagcttatgg actcaagtta gtctttttta tggggaattt ttctttccta
     4741 tcttaatctc aatcttttgt gcatatattt gtaggcttga acatatgaat cataattgga
     4801 ataatgtaat gacactgcca gttaagttga aaaatatctt tttatctaaa cttacagtag
     4861 tttcaatact tacagctatt acacaagttt tctttgttat cttttatata gttgcaggaa
     4921 atatgtttgg tttttctggt agtgtaccta aagaattgac tggttggatt gctaaaggtt
     4981 ggattatttc tatttcaata gcttctatac agttatacat gtctataaag attagaagtt
     5041 ttgcaactcc tattggtatt agtctttgtc tttctctatt tggtatggga ttaatagtag
     5101 caaaacctgc tgttggtata tttttccctt attctatgtt aggtagtgct atgggagtta
     5161 taaagcaatc tgctttaaat gtcaatgaca ctatagttac aatatgtgtt gcatttatta
     5221 ctactatatt attctctgaa attgccaata gaaattttag aaagaatgat gttatttctt
     5281 aatggtaaac gctagatatt tttttgttta aaatttatat tctttttagt tgaaatatat
     5341 atccttttta gttaaaaatc tatatctttt attttaattt tatatgtttt atcta
//
LOCUS       DAEPXF010000227         5288 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain HB27
            SAMN13376389-rid12479423.denovo.235, whole genome shotgun sequence.
ACCESSION   DAEPXF010000227 DAEPXF010000000
VERSION     DAEPXF010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13376389
            Sequence Read Archive: SRR10518432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5288)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5288)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001069624.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001069624.1
            Genome Coverage       :: 315x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/15/2021 01:54:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,165
            CDSs (total)                      :: 4,076
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 89
            rRNAs                             :: 10, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 10 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 19 of 69
            Pseudo Genes (incomplete)         :: 44 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 10 of 69
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5288
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13376389-rid12479423.denovo.235"
                     /strain="HB27"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="China: Hebei"
                     /collection_date="2017"
     gene            194..1720
                     /locus_tag="KRL67_003512"
     CDS             194..1720
                     /locus_tag="KRL67_003512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HBH1524350.1"
                     /translation="MNESYWFLNSSPKEYNKLGENIKTNCLIVGGGITGLTTAYLLAK
                     EGKKVVLVEADKIGYGTSGRNTGKVTCQHDIFYSKIEKKYGLDKAKSYYNANNEALNL
                     VEQIIEENNIKCDFKRETSFVFTEKEDTIKNIKDEYRTCKKIGINCEYHETIENIPLD
                     IKGAISFTNQGQFNPKKYIDGLAKAAVNLGLKIYENTPVVDLEKGKICRVKTREDNII
                     EAENVIISSHSPWYDGLNFYFAKEYAERAYLMAAVLENKLADGMFISIDDPSITFRQY
                     NDGNENLLIFGGGDHKVGQGGTEKEIFDDLEHYGKKVFKVKDFKGKWSAQDNMSFDNV
                     PYIGYINKREDHIYVATGFSKWGITNGTAAGIIIKDLIINNNSDYKDTFNPSRLGSYF
                     SKDFIKENANVAINYVSGKLKIGSGDMPKNNGEGKIVNIDGKRYGVYKDDNGDFYIVD
                     TTCTHLGCELNFNSEEKTWDCPCHGSRFDYKGNILEGPALKPLKLYGHGDNDVNPKLL
                     "
     gene            1748..2305
                     /locus_tag="KRL67_003513"
     CDS             1748..2305
                     /locus_tag="KRL67_003513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH1524351.1"
                     /translation="MNKLLRPITILLYIVYILSAVFFVANKEYGSLGLVAISLAGTFI
                     LSFINKQTERLLSKELYIILALFIMFSSLLGTCFNFYDINYYDDFLHLWSGIISCIVA
                     FSIIKYFYTQREISKMSKLFLVIFLFMFSMGVASLWEIGEFLMDTFIGTTTQAGGLED
                     TMIDMVDALIGTIITIPFIVKKSSI"
     gene            2827..3870
                     /locus_tag="KRL67_003514"
     CDS             2827..3870
                     /locus_tag="KRL67_003514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH1524352.1"
                     /translation="MLKKWFGIVKKRQKSESVKEEGEVILKNEKILSEEKSIDEEGVV
                     VNIDNEILTKVEVVNDDNEIEEKIIEEDWLISENTLKLDDKEAIINDKDIELCKKEVQ
                     VESEKIGLNKFEELDQNENYNLEKNVIEEKEVSECLTEEDLEYIKEIKIKRGKSIKAI
                     NLYTKEEWFFDTHIQCSKKLKVPLGYIRENLKYGYMDYFGDAINYLSEVLNIDEYCKN
                     EWSYLDNSKSPSEIFNILNNKIFSIRLSNEKRNEILTNDKIEALKMNYRFECIDEEYD
                     EYFKKYKSIIKRGGKKKVELVNKKGDVLEIFKSLEECAIYLQKEKNEVIQMLKYGDTK
                     VGRNFIRYSLRSI"
     gene            3981..5123
                     /locus_tag="KRL67_003515"
     CDS             3981..5123
                     /locus_tag="KRL67_003515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HBH1524353.1"
                     /translation="MSEEKNPKENILDESKNSDNVNSKKDDVSDEKNTDNKVKKREVK
                     LNNKTLKAIVAIVAVGAICLGGGYVFGKEAGRKLPATSKHYNSSEVVATIGDKKITGE
                     MLRKRMEPLFYLNAKTTMTDDEIRSYESNMIETMITSDILSSEAAKEKISITNDQLDA
                     QYNNLMSSIESAMGMTKEEFLKQFDLTEKYIKDDMKKELVASKYLNEKSKVTDKEAEN
                     YYNKNKNNYLQVRASHILIKTVDDKGKQVSNSKKAELKKEAEEILKKAQAGEDFATLA
                     KKYSEDSSAESGGDLGFFGKGQMVESFEKAAFALKKGEVSNKLVESDYGYHIIKKTDE
                     KYQPFEEVKSDLVSNLTSEKQNLLIQNLKEKYNVKITDKYQMKAKE"
BASE COUNT         2237 a          430 c          937 g         1684 t
ORIGIN      
        1 gaattataag agtttagtca gtagtagtgt gagtaataag gactttataa atacatagaa
       61 atataatgat attaatatag ctaagtgatt tttaatttag ctttttatta agcttatttt
      121 ttatactatt tataataaat aaactccatg atgggaaaac tattactatt taatatgtaa
      181 aggagtattt taaatgaacg aatcatattg gtttttaaac tcaagtccaa aagaatataa
      241 caaattaggt gaaaatataa aaacaaattg tcttatagta ggtggtggaa taactggatt
      301 gacaactgca tatttattag caaaagaagg taaaaaagta gtgcttgttg aggctgataa
      361 aataggatat ggaacttctg gaaggaatac tggtaaggta acttgtcagc atgacatatt
      421 ttactctaag atagagaaaa aatatggatt agataaagca aaatcatatt ataatgcaaa
      481 taatgaagct ttaaatttag ttgaacaaat aatagaagaa aacaatataa aatgtgattt
      541 taaaagggaa acttcttttg tatttacaga aaaggaggat actataaaga atattaaaga
      601 tgaatatcgt acatgtaaaa aaataggaat aaattgtgag tatcatgaaa ctatagaaaa
      661 tattccatta gatataaagg gggctattag ttttactaat caaggtcaat ttaatccaaa
      721 gaagtatatt gatggattgg caaaagcagc agtaaattta gggctaaaaa tatatgaaaa
      781 tacaccagta gtagaccttg aaaaaggcaa aatatgtagg gttaaaacaa gagaggataa
      841 tataatagaa gctgagaatg ttataatttc atcccatagt ccatggtatg atggattgaa
      901 tttttatttt gcaaaagaat atgcagagcg tgcttattta atggctgcag ttttggaaaa
      961 taaattagca gatggtatgt ttataagtat agatgaccca tcaataactt ttagacaata
     1021 taatgatgga aatgaaaatt tactgatttt tggaggagga gaccataaag tcggtcaggg
     1081 aggaacagag aaagaaatat ttgatgattt agagcattat gggaaaaaag tctttaaggt
     1141 aaaagatttt aaggggaaat ggtctgctca agataatatg agttttgaca atgttcctta
     1201 tattggttat atcaacaaaa gagaagacca tatttatgta gctacagggt tctccaaatg
     1261 gggaattact aatggaactg ctgctggaat aataataaaa gatttgataa taaataataa
     1321 ttctgattat aaagatactt ttaatccatc cagattaggt agctactttt caaaagattt
     1381 tataaaggaa aatgcaaacg tagcaataaa ttatgtaagt ggaaaattaa aaataggtag
     1441 tggagatatg cctaaaaata atggagaagg aaagattgtt aatattgatg gaaaaagata
     1501 tggtgtatat aaggatgata atggggattt ttatattgta gacactacat gtactcattt
     1561 aggatgtgaa cttaatttta atagtgaaga aaaaacatgg gattgtccat gtcatggttc
     1621 tagatttgac tataagggaa atatacttga aggaccagct cttaagccat taaaacttta
     1681 tggacatggt gataatgatg ttaatcctaa gttattataa aattattttt aggaaggtaa
     1741 agtgtatatg aataaacttt taaggccaat aactatatta ttgtatatag tttatatttt
     1801 atctgctgta ttttttgtag caaataaaga atatggaagt ctaggtctag tagcaattag
     1861 tttagctggt actttcattt tgagttttat aaataagcaa actgaaagat tattgagtaa
     1921 agaattatat ataatacttg ctttatttat aatgttttca tcattattag gtacttgttt
     1981 taatttttat gatataaatt attatgatga ttttttgcat ttatggtcag gtataatatc
     2041 ttgtattgtg gctttttcta taattaaata cttttataca caaagagaga taagtaaaat
     2101 gagtaaattg tttttagtta tatttctatt tatgtttagt atgggagtag cgagtttatg
     2161 ggaaataggt gaatttttaa tggatacttt tattgggact actacacagg ctggaggatt
     2221 agaagatact atgattgata tggtagatgc tttaataggt acaattataa ctattccttt
     2281 tatagtaaaa aaatctagta tatagtttta aattttttat attagttata atggatttaa
     2341 ttaatcatgt tgagtttcaa ggtctatatg attaattaaa tccatttttg ttatttataa
     2401 ttattattga atatattttt acatatgaga tatacggtga tagatagaaa ttgttggatt
     2461 atgtaaataa ttaatatata ataccattat gggcaataaa tacaaatcaa atgataatat
     2521 agtctattta tgtaaatgtt atattgtatc ctaagtataa tagtaagttt tttgataatg
     2581 gtgtagaaag taaaaatcta ctagtctaaa ttgatgtatg gattagttta ggaacagtaa
     2641 ttgcatatct taaatttgat gtcatgttgg gctactagaa ataggtcatc tttttcactt
     2701 gaatatcttt tctttaaaca tggggagtat catatcaaaa acatatgaaa attaagtgat
     2761 ttataagaac agtaatattt tatatgatag tatagaatag taataaagta ttaggaggaa
     2821 cattgagtgt taaaaaagtg gtttggtatt gtgaaaaaaa gacaaaaaag tgagtcagta
     2881 aaagaagaag gtgaagtaat attaaaaaac gaaaaaatat tatctgaaga aaagtcgata
     2941 gatgaagaag gagttgtggt taatattgat aatgaaatat taactaaagt agaagtagta
     3001 aatgatgata atgaaataga agaaaaaata atagaagaag attggttaat tagtgagaat
     3061 accttaaaat tagatgataa agaagcaatt attaatgata aagacataga attatgtaaa
     3121 aaagaagttc aagttgaaag tgaaaaaata ggtttaaata agtttgaaga acttgaccaa
     3181 aatgaaaatt ataatctaga aaaaaatgtt attgaagaaa aagaagtaag tgaatgtttg
     3241 acagaagaag atttagagta cataaaagaa attaaaataa aaagaggaaa aagtataaaa
     3301 gctataaact tgtatactaa agaagagtgg ttttttgaca ctcatataca gtgtagtaaa
     3361 aaactcaaag ttccattggg gtatataaga gaaaacttaa aatatggata tatggattac
     3421 tttggagatg caataaatta tttaagtgaa gtattaaaca tagatgaata ctgcaaaaat
     3481 gaatggagtt atctcgataa tagcaaatct ccatctgaga tatttaatat tctaaacaat
     3541 aaaatattta gtataagact ttctaatgaa aaaagaaatg aaattttgac aaatgataaa
     3601 attgaagcat taaaaatgaa ttatagattt gaatgtattg atgaagaata tgatgaatat
     3661 tttaaaaaat ataagtctat aatcaaaaga ggtggaaaga aaaaagttga attagtaaat
     3721 aaaaaaggtg acgttttaga aatatttaag tctttagaag aatgtgccat ttatttgcaa
     3781 aaggaaaaga atgaagttat acaaatgtta aaatatggag atacaaaagt aggaaggaac
     3841 tttataaggt atagtttgag aagtatttaa tttcatttta aattcataag agtttcattt
     3901 ctgattcaca catttaatat tattttaatg taaaataata aatgtgaagt attgcacaat
     3961 taaaattaag gagggtatta atgtctgaag aaaaaaatcc aaaagaaaat atattagatg
     4021 aatctaaaaa ttcagataat gtaaattcta aaaaagatga tgtgtcagat gaaaaaaata
     4081 ctgataacaa agttaaaaaa agggaagtaa agctaaacaa caaaacactt aaagctattg
     4141 tagcaattgt tgcagtagga gctatatgtt taggtggagg ctatgttttt ggcaaagaag
     4201 ctggaagaaa attgcctgct acttcaaaac actataatag cagtgaggta gtagctacta
     4261 ttggggataa aaaaataaca ggtgaaatgt taagaaagag aatggagcca ttattttatt
     4321 taaatgcaaa aactactatg actgatgatg aaatacgttc atatgaatca aatatgattg
     4381 aaactatgat tacatctgat atattatcct ctgaagcagc aaaagagaaa ataagtataa
     4441 ctaatgacca actagatgct cagtataata atttaatgag tagtatagaa tcagcaatgg
     4501 gaatgactaa agaagagttt ttaaaacagt ttgatttgac tgaaaaatat ataaaagatg
     4561 atatgaaaaa ggaactagta gcatcaaaat acttaaatga aaaatcaaaa gttactgata
     4621 aagaagcaga gaactattac aataaaaaca agaacaatta tttacaagtt agagcttcac
     4681 atattttaat aaaaactgtt gatgataaag gtaaacaagt tagtaatagt aaaaaagctg
     4741 aacttaagaa agaagctgaa gaaatactta aaaaagccca agctggggaa gattttgcta
     4801 ctctagctaa aaaatactct gaagatagta gtgcagaatc aggcggagat ttaggtttct
     4861 ttggtaaagg tcagatggtg gaatcttttg aaaaagcagc atttgcattg aagaagggag
     4921 aggtatctaa caaacttgta gaaagtgatt atggatatca tataattaag aaaactgatg
     4981 aaaaatatca gccatttgaa gaggtaaaat cagatttagt aagcaattta acatcagaaa
     5041 aacaaaacct acttatacaa aatttaaaag aaaaatataa tgttaaaatt acagataaat
     5101 atcaaatgaa agctaaagaa tagataaagt caatttatta aaaaaaagaa aaattaactg
     5161 tatggataaa agctattttt actctcaata tagagagtct aaaatagctt ttattatttt
     5221 gaaataaaat ctatttatat atatgaagtg ataaagaaat aacatgcaaa aaaatattaa
     5281 aaagaaat
//
LOCUS       DAEPXF010000228         5278 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain HB27
            SAMN13376389-rid12479423.denovo.236, whole genome shotgun sequence.
ACCESSION   DAEPXF010000228 DAEPXF010000000
VERSION     DAEPXF010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13376389
            Sequence Read Archive: SRR10518432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5278)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5278)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001069624.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001069624.1
            Genome Coverage       :: 315x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/15/2021 01:54:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,165
            CDSs (total)                      :: 4,076
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 89
            rRNAs                             :: 10, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 10 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 19 of 69
            Pseudo Genes (incomplete)         :: 44 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 10 of 69
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5278
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13376389-rid12479423.denovo.236"
                     /strain="HB27"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="China: Hebei"
                     /collection_date="2017"
     gene            265..1728
                     /locus_tag="KRL67_003516"
     CDS             265..1728
                     /locus_tag="KRL67_003516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine:cation symporter family protein"
                     /protein_id="HBH1524354.1"
                     /translation="MTDLVNTINGIVWSPVLVAMCLCIGLYFSLKLKFFQIRDVKEMF
                     TLLLEGDSSHVGISSFQGFATSMAGRIGTGNIAGVAVAIAMGGPGALVWMCIMATLGS
                     ATAFVESTLAQIYKDEHDGQYRGGPPYYFEKGLGWKKCSVLFSIVAIISYTFFMPGTQ
                     SNTLYLAMNQAFGFSKMTIAVASSIALAIIIFGGIKRIGLFAEKVVPIMGGAYLIMAL
                     VIIITNISLVPEAIVTMFKSAFGFGSVFGGMLGSAISWGVKRGIYSNEAGEGSGTYGA
                     AAAEVSHPAKQGLVQAFSVYVDTLLICMATGIMIVITGMYNVTGVCTTLPGVEPGPQY
                     VQSAINTLHPQLGDIFIAIVLLLFTFTTLLAFSYMMETNVSYLVTRIKAEKHEKYIIN
                     VCRFMLITCAAMCCFIEPLTSWALGDICIGVLTYINLIAIFTLKKPAIKAYYDYIRQK
                     KMGIKRSDRTFNPIELDIRNAEFWENRHLKEDKHELN"
     gene            1784..2920
                     /locus_tag="KRL67_003517"
     CDS             1784..2920
                     /locus_tag="KRL67_003517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HBH1524355.1"
                     /translation="MKERIIQVADSKKEKILGLCQSLYDEPEIALQEYKSAKKISEFL
                     REEGFDVEENLAGMATAFKATKKNGDGPKIAFIAEYDALPGNGHACGHHLIASMGVGA
                     GIALSSILDTYKGEVSIIGTPAEETGDGKPYLIEHGVFDGYDAAMMIHPNSKTCVAPE
                     IIAIGGLDFIFTGKASHAGAKPYNGINALDAVVLLYNNINALRQQLIDGTRIHGIILE
                     AGTAANVIPDMGKVRLEIRAKEQNYFDEVVEKVKNCARGAAIATGCELEFYHFEPTCQ
                     GLNENKVLSGIFTKIMEEFGIYEDDQVMLGSTDMGNLSQIMPCIHPLMKFSENGEELH
                     TKEFLEASINSYAKDRVIDGIKILALTGFNLFENPELLKKMKEE"
     gene            3156..4271
                     /locus_tag="KRL67_003518"
     CDS             3156..4271
                     /locus_tag="KRL67_003518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="linear amide C-N hydrolase"
                     /protein_id="HBH1524356.1"
                     /translation="METLEVRNIELSGTNYEIGYKLGELVANMPEIIEGQINKSNLVS
                     KKEEKEMLELFDKYCPGLNEELQGFADAIQVNCNQILYYTMTYLKPGCSQVALAPELT
                     ENGHVLFARNFDFSHNMEDFVLCKTKVNGKYAHIGTTIMQFGRGEGMNECGLGVSQSS
                     CGIPVGNSDGLRKPAIVGLQFWAVIRYLLENCKDVDEALEYLKDMPIAYNINLLLADK
                     SGNIALVETLDGKKGVNRISGLENKREHFLHSTNHIHIDKLHKLEPQSMKNSIHRYKL
                     IKEYINKSKKIGEKELMNLLSLKYPNGLSCNYYNDFFGTLKSIVMDLNIGKFNILWGG
                     LENKWESYYLKDDIKSITQRISINIEKAPSDFFDFIE"
     gene            4422..>5278
                     /locus_tag="KRL67_003519"
     CDS             4422..>5278
                     /locus_tag="KRL67_003519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HBH1524357.1"
                     /translation="MDYLKYFEKAVMYIENNLHENITVNDVAKEIGYSYYHLTRLFKS
                     MFGESVGSYIKKRRLVSSTKELLYSDKKVIDIAIGSGFESSEAFSRAFKSIYKVSPIE
                     YRKNRIDVFVGKKKKLELDFMRHLVGNITIKPVIKEIEEIKVIGIKDKVILENNSLPD
                     LWEKFRKIHHIVPNTLPSKRVFGICEAISEIHLVSESMEFNEIIGLEVNSYDVIPNSF
                     VSKTIKEGKYAIFTHTGKLDNLDKTYEYIWGTWFLSSKEELDIRDDFEVYDERFLGPN
                     NVNSQIDIY"
BASE COUNT         2015 a          546 c          999 g         1718 t
ORIGIN      
        1 caggcttttc attatatgta tttcaattta cattgtttga ttattattca aacacacgtc
       61 gttaaaaatt ttaaaaattt atgtttagat atgattaata ctcttgattt tagaaataaa
      121 tatttgtaga ttgtagatta aagataatat aaataaatag aaggtagatt ttttagatat
      181 attaagataa agattatata aaatattgaa atatagctgt ttaaattatt gtctataaat
      241 ataaaatgaa tggtggtgag ttaagtgacc gatttagtta acaccattaa tggaattgtt
      301 tggtcacctg tattagttgc aatgtgtttg tgtataggat tatatttttc attgaaatta
      361 aaattttttc aaataagaga tgtaaaagaa atgtttactc tcttgctcga aggagatagt
      421 tcccatgttg ggatatcatc atttcaaggt tttgctacat ctatggcggg aagaataggg
      481 actggaaata tagctggtgt agctgtagca atagcaatgg gaggtccagg agcattagta
      541 tggatgtgca taatggctac attaggttca gcaactgcat ttgtagaatc tactctagct
      601 caaatatata aagatgagca tgatgggcag tatagaggag gacctccata ttattttgaa
      661 aaaggtcttg ggtggaaaaa gtgttcagtt ttattttcaa tagttgcaat aataagttat
      721 acatttttta tgcctggtac acaatcaaac actttatatc tagcaatgaa tcaagcattt
      781 ggattttcaa aaatgacaat agcagtagca tcttctattg cattagcgat aataattttt
      841 ggaggaatta aaaggattgg tttatttgct gaaaaagtag tacctataat gggtggagca
      901 taccttatta tggcattagt aataattata acaaatatta gtttagttcc agaagctatt
      961 gtaacaatgt ttaaatctgc atttggattt ggttcagttt ttggaggaat gttaggttca
     1021 gcaatatctt ggggagtaaa aagaggtata tactcaaatg aagcaggaga aggttctggg
     1081 acttatggtg cagcagcggc agaagtaagt catccagcca aacaaggatt agttcaagca
     1141 ttttctgttt atgtagacac attattaata tgtatggcta caggtattat gattgtaata
     1201 acaggaatgt ataatgttac aggtgtttgc acaacactgc caggtgtaga accggggcca
     1261 cagtatgtac agagtgcaat aaatacattg catccacaat taggtgatat ttttatagca
     1321 atagttttat tattatttac atttacaact ttattagcct tctcatatat gatggagact
     1381 aatgtatctt acttagtaac tagaattaaa gcagaaaaac atgaaaagta cataattaat
     1441 gtctgtagat ttatgcttat aacatgtgca gcaatgtgtt gttttataga accacttaca
     1501 agttgggcat taggtgatat atgtataggt gtattaacat atataaattt aattgccatc
     1561 tttactctaa aaaaaccagc tataaaagca tattatgatt atataagaca gaaaaagatg
     1621 ggaataaaaa ggagtgatag aacttttaac cctatagaat tagatataag aaatgctgag
     1681 ttttgggaaa atagacactt aaaagaggat aagcatgagt taaattaaaa ttataataaa
     1741 atataaaaat tactaaataa atttagtggg gggtaagatt ggtatgaaag aaagaataat
     1801 acaagtggct gattcaaaga aagaaaaaat tctagggcta tgtcaatctt tatatgatga
     1861 accagaaatt gctttgcaag aatataaatc tgctaaaaaa atatcagaat ttctaagaga
     1921 agagggtttt gatgttgagg aaaacttggc aggtatggct acagctttta aagcaaccaa
     1981 gaaaaatgga gatggtccta aaatagcatt tatagcagaa tatgatgctc ttccaggcaa
     2041 tggccatgca tgtggacatc atttaattgc atctatggga gttggagctg gaatagctct
     2101 ttcaagtata ctggatactt acaagggaga agtaagtata attggaacac ctgctgaaga
     2161 aactggagat ggaaagcctt acttgataga acatggtgta tttgatggct atgatgcagc
     2221 aatgatgata catccaaatt cgaaaacttg tgtagcacct gaaattatag caataggagg
     2281 tctagatttc atatttacag gaaaagcatc tcacgcagga gctaagccat ataacggaat
     2341 aaatgcttta gatgcagtag tactactata taataatata aatgcactaa gacaacaact
     2401 aatagatgga acgagaatac atggtattat actagaagca ggaactgctg caaatgtaat
     2461 acccgatatg ggaaaagtaa gattagagat aagggcaaaa gaacaaaatt actttgatga
     2521 agtagtagaa aaagtgaaaa attgtgctag aggtgctgca atagccactg gatgtgaact
     2581 tgaattttat cactttgaac caacttgtca aggattaaat gaaaataaag ttttatcagg
     2641 tatatttaca aaaatcatgg aagagtttgg catatatgaa gatgatcaag taatgttagg
     2701 ttcaacagat atgggaaatt taagtcaaat aatgccatgt atacatcctt tgatgaagtt
     2761 ttctgaaaat ggagaagaac tacatacaaa agaattttta gaggcttcta taaattcata
     2821 tgcaaaagat agagtaattg atggtataaa aatattagct ttaacaggat ttaatttatt
     2881 tgaaaatcca gagttattga agaaaatgaa ggaagagtag aatttagact atttttaatt
     2941 gtactctttg aaataagaag agtgtctcaa aatgaacttt ttagtttatg agacactctt
     3001 ttttgtatga aattaaatat atttttatca ttttaacaat gaaaaagagg cttatctgtt
     3061 tttatataac cttttttaac ttgtagcaca aaatatcatg aatgattaca attcaaatga
     3121 taaaatcgat tataagatat ataggagggg tacatatgga aactttagaa gtaagaaata
     3181 ttgaattgag tggtacaaat tatgagatag gatacaaact aggagaattg gttgctaata
     3241 tgccagagat aatagaaggt cagattaata aatctaatct ggtaagcaaa aaagaagaaa
     3301 aagagatgtt agagttgttt gataaatatt gtccaggtct taatgaagaa ttgcaaggtt
     3361 ttgctgatgc aattcaagtg aattgtaatc aaattttata ttatactatg acttatctta
     3421 aaccaggttg tagtcaagtt gcattagcac cagaattaac tgaaaatggg catgtactat
     3481 ttgcacgtaa ttttgatttc tcacacaata tggaggattt tgttttatgt aaaactaagg
     3541 taaatggaaa atatgcacat ataggtacta ccattatgca atttggaaga ggtgaaggta
     3601 tgaatgaatg tggcttaggt gtgagtcagt catcatgtgg tattcctgta ggcaattcag
     3661 atggtttgag aaaaccagca attgtaggtt tacagttttg ggctgttatt agatatttat
     3721 tagaaaattg caaagatgta gatgaagcat tagaatattt aaaagatatg ccaattgcat
     3781 acaatataaa tttattactt gcagataagt ctggaaatat tgcacttgta gagactttag
     3841 atggaaaaaa aggagtgaat agaattagtg gattagaaaa taagagggaa cattttttac
     3901 attctactaa ccatatacat attgataaac ttcataaatt agaaccacaa tcaatgaaaa
     3961 attctattca tagatataaa ttaattaaag aatatataaa taagtctaaa aaaattggag
     4021 agaaagagtt aatgaattta ttgtctttaa agtatccaaa tggcttatct tgtaattact
     4081 ataatgattt ttttggaact ttaaaaagta tagttatgga tttaaatata ggaaagttta
     4141 atattttatg gggtggacta gagaataaat gggagagcta ctatttaaaa gacgacatta
     4201 aatctataac acagaggata agcataaata ttgaaaaagc accatctgac ttttttgatt
     4261 tcattgaata aaaaatgtat ttaattattc taaataatta ggctatataa atcaactaaa
     4321 agtttttatt tatcatattg aattagttat aatattttag tatagtaaaa atagtgttat
     4381 tttgcatact taaagagttt aagtagaaga gaggtgccat attggattac ttaaagtatt
     4441 ttgaaaaagc tgtaatgtat attgagaata atttacatga aaatataact gtaaatgatg
     4501 tggcaaaaga aattggatat tcttattatc atttaacacg cttatttaag tctatgtttg
     4561 gtgagagtgt aggaagttat ataaaaaaaa gacgattagt aagtagtaca aaagaacttt
     4621 tatatagtga taaaaaagtg atagatatag ctattggtag tggatttgag tcttcagaag
     4681 catttagcag agcatttaaa tctatataca aggttagccc tatagaatat agaaaaaata
     4741 gaattgatgt atttgttggt aaaaagaaaa aattagaatt agattttatg agacatcttg
     4801 ttggaaatat aacgattaaa ccagttataa aagaaataga agaaataaag gtaataggta
     4861 taaaggataa agtaatttta gaaaataatt cattacctga cttatgggag aagttcagaa
     4921 aaatacatca tattgtccca aatactttgc catcaaagag agtttttgga atctgtgaag
     4981 caatctctga aatacaccta gttagtgaaa gtatggagtt taatgagata atagggcttg
     5041 aagttaatag ttatgatgtt attccaaatt catttgtttc aaaaactatt aaggaaggaa
     5101 agtatgctat atttactcat actggtaaat tagataattt agataaaact tatgaatata
     5161 tatggggtac ttggttttta agctcaaaag aagaacttga tataagagat gattttgaag
     5221 tatatgatga acgattttta ggtcctaata atgtgaactc acaaattgat atttatat
//
LOCUS       DAEPXF010000229         5233 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain HB27
            SAMN13376389-rid12479423.denovo.237, whole genome shotgun sequence.
ACCESSION   DAEPXF010000229 DAEPXF010000000
VERSION     DAEPXF010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13376389
            Sequence Read Archive: SRR10518432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5233)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5233)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001069624.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001069624.1
            Genome Coverage       :: 315x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/15/2021 01:54:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,165
            CDSs (total)                      :: 4,076
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 89
            rRNAs                             :: 10, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 10 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 19 of 69
            Pseudo Genes (incomplete)         :: 44 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 10 of 69
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5233
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13376389-rid12479423.denovo.237"
                     /strain="HB27"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="China: Hebei"
                     /collection_date="2017"
     gene            100..936
                     /locus_tag="KRL67_003520"
     CDS             100..936
                     /locus_tag="KRL67_003520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HBH1524358.1"
                     /translation="MNIKKIFNNNVVVSSLEDGTEIIVTGAGVGFKKKVGDLIDENLI
                     SKKYFVQDDQRDKYNQILNKTSIEYFKISEEIIEKANEVLNTQVNDSIILALTSHIEF
                     AVEREKQGIKLPNLILNETKQLYREEFEFGLWAIDVIEKRIGIKLPEDEAGYIAIHII
                     NGLENSQKDEGINILEFSKQVIQIIEEVHGFKLDVNSLNYTRLITHLKFFVQRILRKE
                     TYNDSDIEDMYKLVNKNYNKPKVCTEEIAALVLDRFEYKISKEEELYLMIHINKITNS
                     GC"
     gene            1289..2866
                     /locus_tag="KRL67_003521"
     CDS             1289..2866
                     /locus_tag="KRL67_003521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HBH1524359.1"
                     /translation="MKKNKLGLWNFFQMLGKTFMFPIALLSVSGMMLGLGAGFTDPKM
                     IEMIPFLGNEYVNLVLNFMLTIGLFAFNNLGALFAMAIPLGLLKKEKEFGAFSGLVGF
                     IAMHIGTNFYLTRADLLVPAEQMSTNGQAMIMGIQTYNTSVLGGIVAGLIVYAIYDKV
                     SNLKIPESLGFYSGPRLVPIVSLIVMSIVGLLIPIVWPPFFNAFQSLGRWISSAGPLG
                     YFSYAVAERVTIPFGLNHLVTSVFRFTPIGGTAVIDGETYFGTLNMFMAYVENNQIIP
                     LDLAGKMEQGKLMIQYGLAGAALAIYRTAKPENRKALKGLLISGVLTVIIGGISEPIE
                     FLFLFISPALFAFHTFMNGLANMVLPYLGVLMGFTGDLIAFISFGVLRGTATGWPIAV
                     LVAALYFAIYYFVFKWAIIKFNIKTPGREDKEIKVSEGNGSDFKNLSTYKGQQMISAL
                     GGKSNILSLDNCVTRLRLKLQDVSLINEDAIKEAGGIAVVKLDEHTIQVIIGTQVYSL
                     RKQMDKAMEGHLEVCND"
     gene            2859..4043
                     /locus_tag="KRL67_003522"
     CDS             2859..4043
                     /locus_tag="KRL67_003522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HBH1524360.1"
                     /translation="MIDFDKKVDRLGTYCTQWDYVEDRFGKKDLLPFTISDMDLEFPF
                     EITEVLQERLNHRVLGYSRWKHDDFRSSVANWYIKRFNCSVDSDWIYYSPSVMYSISK
                     LIEMLSNEGDGILINTPAYNAFYDVITNNNRNIIKSHLVNRDGFYSIDFNDFEKKCKK
                     SKIFILCNPHNPTGRVWSELELVKMIGICKDNDVKIISDDIHMDIVYDNRMTPILNVA
                     KDYLDSVFICTSASKSFNIPALTGSYVIIPNEGIRNKFENITRYRDFVNSPAILAILA
                     TMVCYDNCEYWLDELLKYLKENLKYTIDYIKDNLEPLKLVMPEGCYFAWIDFSHLKIS
                     SEKFQKLLMDIGEVAIMSGSVYGEEGEFYLRLNVACSREKLEDGLNRIKKTMDYIAKI
                     TL"
     gene            4061..4660
                     /locus_tag="KRL67_003523"
     CDS             4061..4660
                     /locus_tag="KRL67_003523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HBH1524361.1"
                     /translation="MGYLKTMVDVMTTEMNAIRCLIDEAGIEYESIVSEIANCEGKVI
                     FMGVGKSAHIGKKLAATFASTGTPSFFVHATEAVHGDLGMIESKDITILISNSGNSME
                     VVNCIKSIKAIGSKTIAFTSNRNSVLAKECDYALIYPAKDEADHLNLAPTTSSTITLV
                     LGDSIACALSKSSNFGSSDFYKYHPGGSLGEKLKTANNG"
     gene            4684..5154
                     /locus_tag="KRL67_003524"
     CDS             4684..5154
                     /locus_tag="KRL67_003524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HBH1524362.1"
                     /translation="MFKIFKKKSIKSPISGKVIELSKIQDVVFSQKIMGEGVAIDSTG
                     DIVYAPINGKIAVVAETKHAFVIELENGMELLIHVGLDTVNLKGEGFEALVSIGEQVK
                     EGTPMLKINRPLIESKGISLITPVTITNHSEYNMNTCNVGNSVEGGKDTVIEFK"
BASE COUNT         1952 a          560 c         1009 g         1712 t
ORIGIN      
        1 caaaatatat acgaaagtaa aattttaaat attgacatta aattaaatat actatactat
       61 gtaagtgtga tgagagaatt tttttagagg ggatttatag tgaatataaa aaagatattt
      121 aataacaatg tagtagtaag ctccctagaa gatggcacag aaataattgt cactggtgct
      181 ggagtaggat ttaagaaaaa agtaggcgat ttgatagatg aaaatttgat ttctaagaaa
      241 tattttgttc aagacgatca aagggacaaa tacaatcaaa tacttaataa gacctctatt
      301 gaatacttta aaatttcaga agaaattata gaaaaggcta acgaagtttt aaatactcaa
      361 gtgaatgatt caataatatt agccttaact agtcacatag aatttgctgt cgaaagagaa
      421 aaacaaggta taaaattacc aaacttaata ttgaatgaga caaaacaact ttatagggaa
      481 gaatttgaat ttggattatg ggcaattgat gtaattgaaa aaaggatagg aataaaattg
      541 cctgaagatg aagcaggata tattgctatt cacataatca atggtttgga aaattctcaa
      601 aaagatgaag gtattaatat actagagttt tcaaaacaag ttattcagat aattgaagaa
      661 gtacatggat ttaaattgga tgtaaattca ttaaattata ctagacttat aactcactta
      721 aagttttttg ttcaaaggat tctccgaaaa gaaacttaca atgatagtga tattgaagat
      781 atgtacaagt tagtgaataa aaattacaat aaacctaaag tgtgtactga agaaattgca
      841 gcattggtat tagacagatt tgaatataag attagtaaag aagaagaatt atatttaatg
      901 attcacataa ataaaataac aaacagtggt tgttaggatt gtaactgata aagcaggcaa
      961 aacctaagtc attgagctta actattagat taagtttttt gatttaggtt tttttattag
     1021 atacaatcaa aatacagaca gtgacttttg ggattgtaac tggttaagca ggcaaaacct
     1081 aaatcattga atttaactat taggttaagt tttttgattt aggttttttt attagataca
     1141 atcaaataca gacggtgact tttaggattg taactggttg agcaggcaaa acctaaatca
     1201 ttgaatttaa ctattaggtt aagttttttg atttaggttt ttctataaat tcgattatgt
     1261 ttttagataa aaataggagg ggaaaattat gaaaaaaaat aagttaggtt tatggaactt
     1321 cttccaaatg ttaggaaaaa cgtttatgtt cccaatagca ctacttagtg tttctgggat
     1381 gatgttagga cttggggcag gttttacaga ccctaagatg attgaaatga ttccattttt
     1441 agggaatgaa tatgttaact tagttttaaa cttcatgtta acaataggat tatttgcatt
     1501 taacaacttg ggagcattat ttgcaatggc aattccacta ggattattaa aaaaagaaaa
     1561 agagtttggt gctttttcag gattagtagg atttatagca atgcatatag gtacaaattt
     1621 ttatttaaca agagctgatt tgttagtacc agcagaacag atgtcaacta atggtcaagc
     1681 tatgattatg ggtatacaaa catataatac aagtgtttta ggtggtattg tagcaggatt
     1741 aattgtttat gcaatatatg ataaagttag taatttaaag ataccagaat cattaggatt
     1801 ctacagtgga ccaagattag ttccaattgt tagtttaata gttatgagta tagttggatt
     1861 attaatacct attgtttggc caccattttt caatgcattc caaagtttag gaagatggat
     1921 ttcttcagca ggaccattag gatatttttc atatgcagtg gcagaaagag ttacaattcc
     1981 atttggacta aatcatcttg taacatctgt attccgtttt acaccaatag gtggaactgc
     2041 tgttattgat ggagaaacgt actttggtac attgaacatg tttatggcat atgtagaaaa
     2101 taatcagatt atacctcttg atttagctgg taaaatggaa caaggtaaat tgatgattca
     2161 atatggatta gctggtgctg cattggctat ctaccgtaca gcaaaaccag aaaatagaaa
     2221 ggcacttaaa ggacttttaa tatcaggagt gttgacagtt attataggtg gtataagtga
     2281 accaatagag tttctattct tattcatatc accagcatta tttgctttcc atacatttat
     2341 gaatggtcta gcaaacatgg tacttccata cttaggcgtg ttgatgggat tcacaggaga
     2401 tttaattgca tttataagtt ttggtgtgtt aagaggaaca gctacaggat ggccaatagc
     2461 agtattagta gcagctttat actttgcaat atactatttt gtatttaagt gggcaattat
     2521 taagtttaat ataaagaccc caggacgtga ggataaagaa ataaaagtat ctgaaggaaa
     2581 tggttcagat tttaaaaatt tgtctacata taaaggacaa caaatgatat ctgctttagg
     2641 tggtaaatct aatatattat cactagataa ttgtgtaact agattacgtt taaaactaca
     2701 agatgtatca ttaataaatg aggatgcaat taaagaggct ggtggtatag cagttgttaa
     2761 attggatgag catacaatac aagtaattat aggaactcaa gtgtattctt taagaaagca
     2821 gatggataaa gctatggaag gtcatttgga ggtatgtaat gattgatttt gataaaaaag
     2881 tagaccgatt gggtacatat tgtacccaat gggactatgt agaagataga tttggtaaga
     2941 aggatttgct tccttttaca atttcagata tggatttaga gtttccattt gaaataacag
     3001 aggtgttgca ggaaagatta aatcatagag ttttgggata cagtagatgg aaacatgatg
     3061 attttagaag ttcagttgcg aattggtaca ttaaaagatt taattgtagc gttgatagtg
     3121 actggatata ttatagtcca agtgtaatgt attcaattag caaacttatt gagatgttaa
     3181 gtaatgaagg agatggaatc cttataaata ctcctgcata taatgctttt tatgatgtga
     3241 taacaaataa caatcgtaat attataaagt cacatcttgt aaatagagat ggattctatt
     3301 caattgattt taatgatttt gagaagaagt gtaaaaaatc taagatattt attttatgta
     3361 atcctcacaa tccaactggt agagtatgga gtgagttaga acttgttaag atgataggaa
     3421 tttgtaagga taatgatgtt aagataatat cagatgatat acacatggat attgtatatg
     3481 acaatagaat gacacctatc ttaaatgttg caaaggacta tttagattct gtattcattt
     3541 gtacatcagc atctaaatct ttcaatatac cagcattgac tggatcatat gtgattatac
     3601 caaatgaagg tattagaaat aaatttgaaa atatcactag ataccgtgat tttgtaaatt
     3661 caccagcaat tctagcaata ttagctacaa tggtctgtta tgataactgc gaatattggt
     3721 tagatgaact attaaaatac cttaaagaga atcttaaata tacgattgat tatataaaag
     3781 ataatttaga accattgaaa ttggtcatgc cagaaggctg ttactttgct tggatagatt
     3841 tttcccacct taaaataagc agtgagaaat tccaaaaatt attgatggat attggagaag
     3901 ttgctattat gtcggggtct gtatatggtg aagaaggtga gttttattta aggttaaatg
     3961 tggcatgttc aagagaaaaa cttgaagatg gtttaaatag gattaaaaaa actatggatt
     4021 atattgcaaa aataacttta tagtaggaag ggagatagtt atgggatatt taaaaacaat
     4081 ggttgatgtt atgactactg aaatgaatgc cataagatgt ttaatagatg aagcaggaat
     4141 agaatatgaa agtatagtta gtgaaattgc aaattgtgaa ggcaaagtaa tttttatggg
     4201 tgtaggaaaa tctgcgcata taggtaagaa attggcagct acatttgcta gtactggtac
     4261 accgagcttt tttgtacatg caacagaggc agtacatgga gatttgggaa tgattgaaag
     4321 taaggatatt acaattttaa tatcaaatag tggaaattct atggaagtgg taaactgtat
     4381 aaaatctatt aaagcaatag gttctaagac aattgcattt acttcaaaca gaaattcagt
     4441 attggcaaaa gagtgtgatt atgctttaat atatcctgca aaggatgaag cagaccattt
     4501 aaatctagca ccaacaacat catctacaat tactttggtt ttaggagatt ctattgcatg
     4561 tgcattatct aagagtagta attttggttc ttcagatttt tataagtatc atccaggagg
     4621 aagtttaggt gagaagttaa aaacagctaa caatggataa aagcaaaaag gagagtagtt
     4681 tttatgttca aaatatttaa aaaaaaatca ataaaatcac ccatatcagg aaaagtaata
     4741 gagttatcta aaatacaaga tgttgtattt tctcaaaaaa ttatgggaga gggtgtagct
     4801 atagattcta caggagatat tgtatatgct ccaatcaatg gaaagatagc agtagtagca
     4861 gagactaaac atgcatttgt aatagaatta gaaaatggga tggaacttct tattcatgtt
     4921 ggattagata ctgtaaattt aaaaggcgaa ggttttgagg cattggtgag tataggagaa
     4981 caagtgaaag aaggaacacc aatgttgaaa attaaccgtc ctttgattga atcaaagggt
     5041 atatcactta ttactccagt tacaataact aatcattcag agtacaatat gaatacatgt
     5101 aatgtaggca atagtgttga aggtgggaaa gatactgtaa ttgagtttaa ataaagaact
     5161 aaaatacaag attaatattt ataaatactt ataaacttat gttttataag attaattgta
     5221 tagatttgtt gta
//
LOCUS       DAEPXF010000230         5092 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain HB27
            SAMN13376389-rid12479423.denovo.238, whole genome shotgun sequence.
ACCESSION   DAEPXF010000230 DAEPXF010000000
VERSION     DAEPXF010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13376389
            Sequence Read Archive: SRR10518432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5092)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5092)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001069624.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001069624.1
            Genome Coverage       :: 315x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/15/2021 01:54:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,165
            CDSs (total)                      :: 4,076
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 89
            rRNAs                             :: 10, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 10 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 19 of 69
            Pseudo Genes (incomplete)         :: 44 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 10 of 69
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5092
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13376389-rid12479423.denovo.238"
                     /strain="HB27"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="China: Hebei"
                     /collection_date="2017"
     gene            70..1953
                     /locus_tag="KRL67_003525"
     CDS             70..1953
                     /locus_tag="KRL67_003525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glycosidase"
                     /protein_id="HBH1524363.1"
                     /translation="MSNKNKENITLSNQIKGTTQNSLTKEAILHIPMSNYAYGYDRET
                     LHIRLRTKKNEAKRVILRIGDQYVWDKGGAGGGNLNASGVGWSGGTNIIMNKEVETEL
                     FDYWIAKCKPLNKRSRYGFIIEGQEEKLLFTEKRIIELGNENDEKELCEIGNFFGYPY
                     LNYVDIPKVPNWVKDTVWYQIFPDRFANGDPSINPEGVDEWGAIPTRDNFTGGDLQGV
                     IEHLDYLSDLGINGIYFCPITIGKTNHRYDTVDYMEVDPALGDKETLKKLIEEAHKRN
                     IKIMLDAVFNHIGYYSKQWQDVVQNKENSRYKDWFYIKDMSKIDTPVEQIDEKNIPYE
                     TFGCEKYMPKLNTENSEVINYLLDVGKYWIQEFDIDAWRLDVSNEVDHVFWRKFREEV
                     KKVKPDIYILGEIWHGSLPWLMGDQFDSVMNYLMSEAMKKFFCTEEINAEEFKYMIND
                     VMVSYPIQVSEVIFNLLGSHDTTRILTYANGNVDRFKLAYMFMFVQTGCPCVYYGDEI
                     GMEGELSSISEGQRKCMEWNEEKWNREIFNFMKKIIRLRKENKELRSISNEWVLADKE
                     NGTIILKKESISIIINNSSKDIILTLPDYLANKKVKDLYEEEIVDLKEELMLEKYKFI
                     ILK"
     gene            2178..2858
                     /gene="pgmB"
                     /locus_tag="KRL67_003526"
     CDS             2178..2858
                     /gene="pgmB"
                     /locus_tag="KRL67_003526"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HBH1524364.1"
                     /translation="MIEAFIFDLDGVITDTAYYHYMAWKKLAHKIGINIDTKFNESLK
                     GISRMESLDRILEFGNKKDLFSKEEKLKMAEEKNNYYISSINDMTSSDILPGIESLLI
                     DVKSNNIKIGLSSASKNAINVLNHLGISDKFDFIANAAKCKNNKPHPDIFLMSAKGLN
                     VNPQNCIGIEDASAGIDAINSANMFSVGVGSYENLKKANLVVDSTNQLKFEYIQEKYN
                     EYIVRRII"
     gene            2855..>5092
                     /locus_tag="KRL67_003527"
     CDS             2855..>5092
                     /locus_tag="KRL67_003527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_097546124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HBH1524365.1"
                     /translation="MKCLFSNVDEWKIIQDKIEIKENRLAESIMSIGNGYMGMRGNYE
                     ENYSGDTHKGSYIAGVWFPDKTRVGWWKNGYPEYFGKIPNSVNYIGVRIFIDDVELDL
                     AKCKVEDFHRELDMKNGILNRRFIVNINDKKFEVRVTRFLSISVKELAVIKYSIKSLN
                     SNTKIKFVPYLDSNIKNEDSNYEEKFWNGISSSSLEYKGSILSRTKENTFNTPVFTVG
                     SMMYIKTNGKVEAISHESDYNYCENSITMDVLKNEVASIEKYVSITSTRDYKESDLLR
                     KCEDILNRELSKSYDDILNEHTKLWNKRWESADVKINGDKSSQQGIRYNLFQLFSTYY
                     GEDSRLNIGPKGFTGEKYGGATYWDTEAFCVPVYLGVADKEVTRNLLEYRYNQLEQAK
                     DNAKKLGLKGALYPMVTFDGVECHNEWEITFEEIHRNGSIVYAIYNYTNYTGDYKYIK
                     EKGIDVIIEIARFWASRVHLSTKKDLYMIHGVTGPNEYENNINNNWYTNYVAKWCLDY
                     AVENILRLEKECNECIDRNNVTREEIEFWKIISNKMYLPYDEELQIIIQNDNFLDKEF
                     VEVKDLPKENFPLNQNWSWDKILRSCFIKQADVLQGIYYFGNKFTKEEKKRNFDFYEK
                     YTVHESSLSSSIYSIIASEIDNLEKAYELYSRTARLDLDNYNNDTNDGLHITSMSGSW
                     LSIVHGFAGMRTWDETLSFDPKLPKEWKNYSFNINYRDNIINVDVCSDLIKIENLKGK
                     PIKIIV"
BASE COUNT         2112 a          460 c          958 g         1562 t
ORIGIN      
        1 atataatagt aaaatatatt aacctatctt tagagtatat aaattattat aaaggaggga
       61 aattttaaaa tgtctaataa aaataaagaa aatataactt taagtaatca aatcaaagga
      121 acaactcaaa atagtttaac aaaagaagct atattacata taccaatgag caattatgcc
      181 tatggttatg atagagaaac actacatata agacttagaa ctaaaaaaaa tgaagcgaaa
      241 agagtaattt tacgaatagg agaccaatat gtgtgggata aaggaggtgc aggaggagga
      301 aatttaaatg catctggagt tggttggtct ggtggaacaa atataataat gaacaaagaa
      361 gtagaaactg aattatttga ttattggata gctaagtgca agcctttaaa taaacgttca
      421 cgatatggtt ttataataga aggtcaagaa gaaaaacttt tatttacaga aaaaagaata
      481 atagagttgg gaaatgaaaa tgatgaaaag gaattatgtg aaataggtaa tttttttgga
      541 tatccatatt taaattatgt agatatacct aaagtgccaa attgggtgaa agatactgtt
      601 tggtatcaga tatttccaga tagatttgca aatggtgacc cgtctataaa cccagaagga
      661 gtagatgaat ggggagctat tccaaccaga gataatttta caggtggaga tttacaaggt
      721 gtgatagaac acttagatta tttgagtgat ttgggaataa acggaattta tttttgtcct
      781 attacaattg gaaaaaccaa tcatagatac gatacagtag attatatgga ggtagaccct
      841 gcattaggag ataaagagac tttaaaaaaa ctaatagaag aagcacataa gagaaatata
      901 aaaataatgt tggatgctgt ttttaatcat ataggatatt attctaaaca gtggcaagat
      961 gtagttcaaa ataaggaaaa ttctcgatat aaagactggt tttatataaa agatatgagt
     1021 aaaattgata ctccagtaga acagatagat gaaaaaaata taccttatga aacctttggg
     1081 tgtgaaaaat atatgcctaa acttaataca gaaaattcag aagtaataaa ttatttattg
     1141 gatgtaggca agtactggat acaggaattt gatatagatg cgtggagatt agatgtttca
     1201 aatgaagtag accatgtttt ttggagaaag tttagagaag aagtcaaaaa agtaaaacca
     1261 gacatttata tattgggaga aatttggcat ggtagtttac catggctaat gggtgaccaa
     1321 tttgattcag ttatgaatta cttgatgtca gaagccatga aaaaattctt ttgtacagag
     1381 gagataaatg ctgaagagtt taagtatatg ataaatgatg taatggtaag ttatccaata
     1441 caagttagtg aagttatttt taatttactt ggaagtcacg atactacaag aatattgact
     1501 tatgctaatg gaaatgttga tagatttaaa cttgcatata tgtttatgtt tgtacaaacg
     1561 ggatgtcctt gtgtatacta tggtgatgaa attggaatgg aaggagaatt aagttcaata
     1621 tctgaaggtc aaagaaaatg tatggaatgg aatgaagaaa aatggaatag agagatattt
     1681 aattttatga agaagattat taggcttcgt aaagaaaata aagagcttag gtctatatca
     1741 aatgaatggg tgttagcaga taaggaaaat ggaacaatta tattgaaaaa agaatctatt
     1801 tctataataa taaataattc atctaaagat ataattttga cgttaccaga ttatttagca
     1861 aataaaaaag taaaagattt atatgaagaa gaaatagttg atttaaaaga agaactaatg
     1921 ttagagaaat acaagttcat tattttaaaa taatacaaaa aagtatttat tttagatttc
     1981 atgttaaatt attatacagt ttaaacaaat ttttatgaac gataaaaaaa tatggtctac
     2041 aagtgagtga attgaaagta gattttaaat taaatactga gttagagtag ataattttat
     2101 aaataatatc ttaaatacaa gatagaaaat aagttgatta gtagtctaaa aaattcttgt
     2161 agaaagagga aataagaatg attgaagcat ttatatttga tttagatggt gtaattacag
     2221 acactgcata ttatcactat atggcttgga aaaagctagc acataagatt ggtataaata
     2281 tagatacaaa atttaatgaa tctttaaaag gcataagtag gatggaatca ctagatagaa
     2341 tattagaatt tggaaacaaa aaagatttat tttcaaaaga agaaaaattg aaaatggcag
     2401 aagaaaaaaa taattattat atatcatcaa taaatgatat gacttcgagt gatattttgc
     2461 cagggataga aagcttgtta attgatgtaa aatctaataa tataaagata ggtctatctt
     2521 ctgcaagtaa gaatgcaata aatgtattaa atcatttggg tataagtgac aaatttgatt
     2581 ttatagctaa tgcagcaaaa tgtaaaaaca ataaaccaca tccagatata tttcttatgt
     2641 cagcgaaagg gcttaatgta aatccacaaa attgtatagg gatagaggat gcaagtgcag
     2701 gtatagacgc tataaactca gctaatatgt tttctgttgg tgttggaagt tatgaaaact
     2761 taaaaaaagc aaatttagtt gtagattcaa caaatcaatt aaagtttgaa tatatacaag
     2821 aaaagtataa tgagtatata gttaggagaa ttatatgaaa tgtttattta gtaatgtaga
     2881 tgagtggaag attatacaag ataagattga aatcaaagaa aatagattag cagagtccat
     2941 aatgagtata ggcaatggat atatgggtat gagaggaaac tatgaggaaa attatagtgg
     3001 agatacacac aaggggtctt atatagctgg agtatggttt ccagataaaa ctcgtgttgg
     3061 ttggtggaaa aatgggtatc cagaatactt tggtaaaatt cctaattcag tcaactatat
     3121 aggtgtaaga attttcatag atgatgtaga attagattta gcaaaatgta aagtagaaga
     3181 ttttcataga gaattagata tgaaaaatgg tattttaaat cgtaggttta ttgtaaatat
     3241 aaatgataaa aaatttgaag tgagggttac taggttttta agtatatctg ttaaggaact
     3301 cgcagtaatt aaatacagta taaaatcatt aaattctaat actaaaataa aatttgtacc
     3361 atatttagat tcaaatataa aaaatgaaga ttcaaactat gaggaaaagt tttggaatgg
     3421 aatttcttca agttctttag aatataaagg gagtattctt tcacgaacaa aggagaatac
     3481 atttaataca ccagtattca ctgtgggttc aatgatgtat attaagacaa atggcaaagt
     3541 agaagctata tctcatgaga gtgattataa ttactgtgaa aattcaataa caatggatgt
     3601 tttaaaaaat gaggttgcaa gtatagaaaa atatgtatct ataacttcaa ctagagatta
     3661 taaggaaagt gacctattaa gaaaatgtga agacatttta aatagagaac tatctaaaag
     3721 ctatgatgat atattaaatg aacatacaaa attatggaat aaaagatggg aaagtgcaga
     3781 tgtaaaaata aatggagata aaagtagcca acaaggtata agatataatt tattccaact
     3841 attttcaaca tattatggag aagattcccg attaaatata ggtccaaaag gatttactgg
     3901 tgaaaagtat ggaggagcta cttattggga tacagaagcg ttttgtgttc cagtatatct
     3961 aggtgtagca gataaggagg taactagaaa tcttttagaa tataggtaca atcagttaga
     4021 acaggcaaag gataatgcaa aaaaactagg gcttaaaggt gctttgtatc caatggtaac
     4081 atttgatgga gtagaatgtc ataatgaatg ggagataaca tttgaggaaa tccaccgaaa
     4141 tggaagtata gtatatgcaa tatataacta tacaaattat acaggtgact ataaatatat
     4201 aaaagaaaaa ggaatagatg taataataga aatagctaga ttttgggcaa gtagagttca
     4261 tttgtctaca aaaaaagatt tgtatatgat acatggagta acagggccaa acgagtatga
     4321 aaacaatatc aataataatt ggtatactaa ttacgtagca aaatggtgtt tagattatgc
     4381 tgtagaaaat atcttacgat tagagaaaga gtgtaatgaa tgtattgaca gaaataatgt
     4441 gactagggaa gaaattgagt tttggaaaat aatatcaaat aaaatgtatc tgccatatga
     4501 tgaggaacta caaataataa ttcaaaatga taatttttta gataaagaat ttgtagaagt
     4561 aaaggattta ccaaaagaaa attttcctct taatcaaaat tggtcttggg ataagattct
     4621 taggtcgtgt tttataaaac aagcagatgt attacaggga atttactatt ttggaaataa
     4681 atttactaaa gaagagaaaa agagaaattt tgatttctat gaaaaatata ctgttcatga
     4741 atcttcttta tcttctagta tctattctat tattgcctct gaaatagata acttagaaaa
     4801 ggcttatgaa ttatactcaa gaacagctag gcttgattta gataattaca ataatgatac
     4861 aaatgatgga ctacacataa cttcaatgtc tggttcgtgg ctttccattg tacatggatt
     4921 tgcaggtatg agaacgtggg atgaaacact atcttttgac cctaagttgc caaaagagtg
     4981 gaaaaattat tctttcaata taaattatag agataatatt attaatgtag atgtatgtag
     5041 tgatttaatt aaaatagaaa atttaaaagg taaaccaata aaaattattg ta
//
LOCUS       DAEPXF010000231         5073 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain HB27
            SAMN13376389-rid12479423.denovo.239, whole genome shotgun sequence.
ACCESSION   DAEPXF010000231 DAEPXF010000000
VERSION     DAEPXF010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13376389
            Sequence Read Archive: SRR10518432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5073)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5073)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001069624.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001069624.1
            Genome Coverage       :: 315x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/15/2021 01:54:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,165
            CDSs (total)                      :: 4,076
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 89
            rRNAs                             :: 10, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 10 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 19 of 69
            Pseudo Genes (incomplete)         :: 44 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 10 of 69
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5073
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13376389-rid12479423.denovo.239"
                     /strain="HB27"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="China: Hebei"
                     /collection_date="2017"
     gene            103..996
                     /locus_tag="KRL67_003528"
     CDS             103..996
                     /locus_tag="KRL67_003528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HBH1524366.1"
                     /translation="MKNKKFIVIILIISILIGVLVKEYSSREINKADINVSKYIKYAD
                     LASKNNAQVNWKYVASIVAVLNKNNLKNVKDSQIQEVSDLFVKNFSKNNKINKLSDVL
                     DELEFSNRQKRLVDNYIDNLKDYGIKPERLKSDTKYMKFIDEIKTEAIQNYKDYKILP
                     SITIAQAIIESSWGKSTLAKQYNNLFGIKADAYWKGKSVTLETKEHLDTIIDDKFRIY
                     DDKNESIKDHAKFLATNKRYKNNGVFDAKTYIYQAKALEKAGYSTAKDENGNSIYAAR
                     LIELIKQYNLQLIDSEIQSEV"
     gene            1077..3047
                     /locus_tag="KRL67_003529"
     CDS             1077..3047
                     /locus_tag="KRL67_003529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH1524367.1"
                     /translation="MVVVTTIIGCSSGEVIKNTSPINSVKNLDFSFEVKPEIFKVSVE
                     SGGSKERKVSNFKKNKTEASWTYPDENIDINIKKEDSYLDVNIKSNLEKETSFNWPKV
                     EGESYILPLYEGKFIPSNDKYWKEYLNESTYNVIESFSMQFFAVNKAKYSVIYIIENE
                     FNNVIEFSSKDKINFTFKHEYPSINKNKEYGFKIYLTNKNITDVAKTYKNYIVDKGEF
                     KTLKEKSKENKNIEKLYGAPHIYFGDKSIITINDIEWNKLKENLSDDLTNWMKELLSM
                     KSGNSEFIKVINQIKTQDYIDKYQKNQIVQAFNEVMQMNTFYNEKVFESKDREIKKVL
                     DKGISKLSRVELIEFNKKLLKSKLEESVNDVATWAKYNTTDVINDINKSGIKNAWIGF
                     DDMEEGYISLEMVKRANELGYLVGPYDSYHSIHKPGEEKWSTASFKDKSLYENATIEN
                     KKGEKETGFQGEGRKLNPTLSLPSVKERVQSILDEGYEFNSWFIDCDATGEIYDDYSQ
                     NHITTQTQDLNARLERMSYIRDHANMVIGSEGGNDFSSSTIAFAHGIETPAFAWVDKD
                     MSKNKDSEYYVGKYYSPTGGVPEIFSKQIPVKERYKKIFIDPIYSLPLFRLVYNDSVI
                     SSHQWLWGTLKIKDEVGSRMMKEVLYNTSPMY"
     gene            3048..3320
                     /locus_tag="KRL67_003530"
     CDS             3048..3320
                     /locus_tag="KRL67_003530"
                     /inference="COORDINATES: protein motif:HMM:NF022752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH1524368.1"
                     /translation="MDRTEWEKHKDKIISHNKVWSAFNKKAIKKEMTDFKFISEDRLV
                     QMTEYGEDLKVLVNFSQKDIKYNDEVIKANSLVIYDADSKVIYKPE"
     gene            3554..4048
                     /locus_tag="KRL67_003531"
     CDS             3554..4048
                     /locus_tag="KRL67_003531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH1524369.1"
                     /translation="MYENNKVIYNFERCEKLENALYLSQFNTYKNFKLTSKKTVINSL
                     DTSTYNMNIHSISLVDTMECISSEDEQMTGKKLILVGDIDTDVTLDYVGNKRNRSSSK
                     KNILKLKIPFSTFIQMPRKIENKDKINLKYLIQDMTSSILDDNNLFISVTAIISYENS
                     SKIE"
     gene            4076..5023
                     /locus_tag="KRL67_003532"
     CDS             4076..5023
                     /locus_tag="KRL67_003532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF11 domain-containing protein"
                     /protein_id="HBH1524370.1"
                     /translation="MRITKTTFKDVFSNDVNGSILKVEKYVDKKCASVFDILTYTVTV
                     TNTSRYKTGNIFFKDYISKYIEFINNTVKVNGIIKRGLNPQQGFYIGRIDASCKKVIS
                     FKSVVLPNSAHRIIKNSANIYYYYKCNLDKFPTRISVESNRVYTNVNKIVFKQLNISN
                     TLKAPKNLRDILKVNVNSKIFDVKPMKNLINKGSNLKLCNLIVFGCVEVEIDYSCKKR
                     SNFKTANKANDIQKDKYYKNDIQNNNENINDCKKQISFGDNKVKKITKTFGFSCFICS
                     PVGIIYEDMKNINIKIEHTSINELNPEELFVNTSLLLYY"
BASE COUNT         2173 a          461 c          835 g         1604 t
ORIGIN      
        1 tatttcataa atcttcataa aatcttcaaa atttcgtaaa gaatatgtca caaaagtaag
       61 gtatattata ttcaacagat aatataaggg aggacaatat ttatgaagaa taaaaaattc
      121 attgttataa tactgataat atcaatttta ataggtgtat tagtgaagga atattcatct
      181 agagagataa ataaggctga tataaatgtc agtaagtata taaaatatgc tgatttagca
      241 agtaaaaata atgcacaagt taactggaag tatgtagctt ccattgttgc tgtgttaaat
      301 aaaaataact taaaaaacgt taaggattca cagatacaag aagtatcaga tttatttgtt
      361 aagaattttt ctaaaaataa taaaataaat aagttaagtg atgttttgga tgagttagaa
      421 tttagtaata gacaaaaaag attggttgat aattatatag acaatcttaa ggattatggt
      481 ataaaaccag aaagattgaa gtctgataca aaatatatga agtttattga tgaaattaaa
      541 acagaagcaa tacaaaatta taaggattat aaaatacttc catctataac tattgcacaa
      601 gctataatag aatctagctg gggtaagtca actttggcaa aacaatataa caatctattt
      661 ggtataaagg ctgacgctta ttggaaaggt aagtcggtga ctttagaaac gaaagaacat
      721 ttagatacaa ttatagatga taaatttaga atatatgatg ataaaaatga atctataaaa
      781 gaccatgcaa aatttttagc cacaaataaa aggtataaaa ataatggtgt atttgatgca
      841 aaaacgtata tatatcaagc taaagcattg gaaaaggcag gttatagtac tgcaaaggat
      901 gaaaatggaa atagtatata tgcagctcgc ctaatagaat taattaaaca gtataatctt
      961 caattaatag acagcgaaat tcaaagtgaa gtgtaacttt tatgtaacat aggagtgata
     1021 tatttatcga ggaggtggct tttatgaaaa aaatattctc gatatttata agttgtatgg
     1081 ttgttgtaac tactattatt ggatgtagta gtggtgaggt gataaaaaat acatcaccta
     1141 tcaatagtgt taaaaatttg gattttagtt ttgaggtgaa acctgaaata tttaaggtat
     1201 cagttgaatc tggtggttca aaagaaagga aagtatcaaa ttttaaaaag aataagacag
     1261 aggcatcgtg gacgtatcct gatgaaaata tagacataaa tataaaaaaa gaggacagct
     1321 atttagatgt aaatattaaa tctaatttag aaaaagaaac tagttttaat tggcccaaag
     1381 ttgaagggga aagttatata ctaccacttt atgagggtaa gtttatacca agtaatgata
     1441 agtattggaa ggagtatcta aatgagtcaa cttataatgt tatagaatct ttttcaatgc
     1501 aattctttgc agtaaacaag gcaaagtatt ctgtgattta tattatcgaa aatgaattta
     1561 acaatgtaat tgaatttagt tctaaagaca agattaattt tacatttaaa catgaatatc
     1621 caagtatcaa taaaaataag gagtatgggt ttaaaatata tttgactaat aagaatatta
     1681 cagatgtagc aaaaacttat aaaaattata tagttgataa aggagaattt aaaacattaa
     1741 aagaaaaatc taaggaaaat aaaaatattg aaaaacttta tggagcacca catatttatt
     1801 ttggagataa gtctatcatc acaataaatg atattgaatg gaataaatta aaagaaaatt
     1861 tatcagatga tttgactaat tggatgaaag agcttttaag tatgaagtct ggcaatagtg
     1921 aatttataaa agtaattaat caaataaaaa ctcaagatta tatagataaa tatcaaaaaa
     1981 atcaaatagt acaagcattt aatgaagtta tgcagatgaa tactttttat aatgaaaaag
     2041 tttttgaaag caaagataga gaaataaaaa aagtattaga taaaggtata tctaaattaa
     2101 gtagggtaga acttatagag tttaataaga aattattgaa atcaaaactg gaagagagcg
     2161 tgaatgatgt tgctacatgg gcaaaatata atacaactga tgtaattaat gatattaata
     2221 agtctggtat taaaaatgcg tggataggat ttgatgatat ggaggaaggt tatatatcct
     2281 tagagatggt taaaagagct aatgagttag gttatctcgt agggccatat gattcatacc
     2341 actctataca caaaccaggg gaagaaaagt ggagtacagc atcatttaaa gataaatctc
     2401 tatatgaaaa tgctaccata gaaaataaaa aaggtgaaaa agaaactgga tttcaaggag
     2461 aaggaagaaa actaaatcct acattgtctt taccaagtgt aaaagagaga gtacaaagta
     2521 tattggatga aggatatgaa tttaattctt ggtttataga ttgtgatgct acaggagaaa
     2581 tctatgatga ttattctcaa aatcatataa caacacaaac acaagattta aatgctagac
     2641 ttgaaagaat gtcatatata agagaccatg caaatatggt cataggttca gaaggtggaa
     2701 atgatttttc aagtagtact atagcatttg cacatggtat tgagacacca gcatttgctt
     2761 gggtagataa agatatgagt aagaataaag acagtgaata ttatgttggt aaatactatt
     2821 ctccaacagg aggtgtacca gaaatatttt caaagcagat acctgtaaaa gaaaggtaca
     2881 aaaaaatatt tatagaccca atttactctc tacctttatt tagattagtt tataatgatt
     2941 ctgtgatatc ttcccatcag tggctatggg gaactttgaa gattaaggat gaagttggaa
     3001 gtagaatgat gaaagaagta ttatataata catcacctat gtattagata gatagaactg
     3061 aatgggaaaa acataaagat aaaataatct cacacaataa agtatggtca gcgtttaata
     3121 agaaagctat taaaaaagaa atgacagact ttaaatttat ttctgaggat agactagttc
     3181 aaatgactga atatggagaa gatttaaagg tattagttaa tttttctcaa aaagacatta
     3241 agtataatga tgaagtcata aaagcaaatt ctttggtaat atatgatgct gatagtaaag
     3301 tcatatataa accggaataa tagatttaat gaaaataaag attgtttaaa tttcaagtaa
     3361 aaaataatat aatattcatt tagtttgtat taaagtagtg actatattaa tctggataat
     3421 tagtatagtc actattattt atattctttc ctaaaagtaa gtagtaactg aatcgtgaat
     3481 aatcaaaaat ggtaatttga aatagttttt atgaatagta ttaatatata gatataggga
     3541 gattgatgtg attatgtatg aaaataataa ggtaatatat aattttgaaa gatgtgaaaa
     3601 gctagaaaat gctctttatt tatctcaatt taatacatat aaaaacttta agttaacttc
     3661 taaaaaaact gtaataaact cattagatac atctacttat aatatgaata ttcatagcat
     3721 aagtctcgtt gatactatgg aatgtatatc gtcagaagat gaacaaatga caggaaagaa
     3781 gttgatttta gttggcgata tagatactga tgtaacatta gattatgtag gcaataaaag
     3841 aaatagaagt tctagtaaaa aaaatatact taaattaaaa ataccattta gtacatttat
     3901 acaaatgcct agaaagatag aaaataaaga taaaattaat ttaaagtatc ttatacaaga
     3961 tatgacttca tctattttag atgataataa tttatttatt agtgttactg caattataag
     4021 ttatgaaaat agctccaaaa tagagtaatc tttaaattat agaaaggtgg atgcaatgag
     4081 aataacgaaa actactttta aagatgtatt ttcaaatgat gtaaatggaa gtattttaaa
     4141 agtagaaaaa tatgttgata aaaaatgtgc atctgtattt gatattttga cctatactgt
     4201 tactgttaca aatacgagta ggtataaaac tggaaatata tttttcaaag attatatctc
     4261 taaatatata gagtttataa ataatactgt aaaagttaat ggtattataa agcgtgggct
     4321 aaatccacaa caaggttttt acataggaag aatagatgca agttgtaaaa aagttatatc
     4381 ttttaaaagt gtagttttac caaattctgc acataggatt ataaagaata gtgctaatat
     4441 atactattac tataagtgca atctagataa attcccaaca agaatatcag tagaaagtaa
     4501 tagagtttat actaatgtaa ataaaattgt attcaaacaa ttgaatataa gtaatacttt
     4561 aaaagctcct aaaaatctaa gagatatatt aaaagtaaat gtaaatagta aaatatttga
     4621 tgttaaacct atgaaaaatc ttataaacaa aggtagtaat ttgaaattat gtaacttgat
     4681 agtttttggg tgtgtagagg ttgaaataga ctatagttgt aaaaagagga gtaattttaa
     4741 aacagcaaat aaggctaatg atattcaaaa agataagtat tataaaaatg atatacaaaa
     4801 taacaatgaa aatataaacg attgtaaaaa gcaaataagt tttggtgata ataaggtaaa
     4861 aaaaataaca aaaacttttg gattttcctg tttcatttgt tctccagttg gaattattta
     4921 tgaagatatg aaaaatataa atataaaaat tgaacataca tcaataaatg aattaaatcc
     4981 agaagaatta tttgtaaata catcactttt attgtattat taagtatttt aaagctaaaa
     5041 cttaaactta ttaaaatagc atttggctaa agg
//
LOCUS       DAEPXF010000232         5067 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain HB27
            SAMN13376389-rid12479423.denovo.240, whole genome shotgun sequence.
ACCESSION   DAEPXF010000232 DAEPXF010000000
VERSION     DAEPXF010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13376389
            Sequence Read Archive: SRR10518432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5067)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5067)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001069624.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001069624.1
            Genome Coverage       :: 315x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/15/2021 01:54:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,165
            CDSs (total)                      :: 4,076
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 89
            rRNAs                             :: 10, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 10 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 19 of 69
            Pseudo Genes (incomplete)         :: 44 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 10 of 69
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5067
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13376389-rid12479423.denovo.240"
                     /strain="HB27"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="China: Hebei"
                     /collection_date="2017"
     gene            201..2702
                     /locus_tag="KRL67_003533"
     CDS             201..2702
                     /locus_tag="KRL67_003533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-translocating P-type ATPase"
                     /protein_id="HBH1524371.1"
                     /translation="MSSNKVVESYKITGMTCAACAKAVERVTKKMDGVYDQSVNIATE
                     KLKIEYDNSKVNFDDIKQVVEKAGYGIIKEESNKKIDMKIDGMTCAACAKAVERVVKK
                     LDGVESISVNIATDKANIDYDPSKVKLSQIKAAIEKAGYKPIEEVRNKVDVDEDKLRK
                     EREMKSLFVKFIVAIVFAVPLFYIAMGPMIIKPIGPWPLPEIINPMTNTFNYALIQLI
                     LVIPVMIAGYKFYINGFKSLFSLSPNMDSLVAIGTLAAFLYSLYTTLQISNGQIQGMH
                     HHQLYYESAGIIIALILLGKYLESKSKGKTSEAIKKLMGLQPKTAIVLVDGKEIETPI
                     EEVEIGDILLVKPGTKIPVDGVVIEGYTSVDESMLTGESIPVEKNVGSKVTGASINKN
                     GVIKFKAEKIGGDTALAQIIKLVEDAQGTKAPIAKLADTVSGYFVPIVIAIAVVASLL
                     WFLIGGKDIVFVLTIFISVLVIACPCALGLATPTAIMVGTGKGAENGILIKGGEALES
                     AHKVNTVIFDKTGTITEGNPKVTDIVLNNNVKEEYLIKIASSAEKGSEHPLGEAIVKY
                     GEEKNIKFEKVDNFKAIPGAGIQVTINDESILLGNRKLMNDNNIKLGDLEEKSNVLAS
                     QGKTPMYIAVDGNLSGIIAVADVVKESSKKAIEILHDMGIKVAMVTGDNAKTANAIAN
                     QVGIDMVLAEVLPEDKSKEVEKLQNQGKFVAMVGDGINDAPALAKADIGIAIGSGTDV
                     AIESADIVLMKSDLIDVPTAIKLSHETIKNIKQNLFWAFGYNTIGIPVAAGILYVFGG
                     PLLNPMIAAAAMSLSSVSVVSNALRLKNFKAYKRD"
     gene            2862..3530
                     /locus_tag="KRL67_003534"
     CDS             2862..3530
                     /locus_tag="KRL67_003534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBH1524372.1"
                     /translation="MNSSILVIEDDSNIQELISEFLSAEGYQVDTANDGLEGIQKFKQ
                     GSYDLVILDIMMPNLDGYGVCKMIRKSSSVPIIFLTALNDEGDQLKGFDLECDDYITK
                     PFSFNLLIKRVEAILRRSNKTINDKFIVFEKLKLDLNTYIAEIDGEPIELTLKEFNIL
                     KALIEKYPQVITREGLLDSIWGYDYYGDTRIVDAHIKNIRKKISLPYIKTVKGIGYTL
                     EKDI"
     gene            3514..4791
                     /locus_tag="KRL67_003535"
     CDS             3514..4791
                     /locus_tag="KRL67_003535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase"
                     /protein_id="HBH1524373.1"
                     /translation="MRRIFDKWEKLSIKYKLFSITTSLLIALALIIYLILYFLLPSYY
                     HEYKIESLQESLKSLVDSSIHFDTYTLEERLYYMAKDQNLAILLKDDQGKIVYGKNEV
                     VILRYSKYMINSLEDEYRTSIPIYTKDAKDGPYTLELVMPLQPIDEANEVIRKLMPYI
                     ISIAILIAIIGAYIYSIVITKPLINIIESEREQEYRRKDFVATISHELKTPITIISGQ
                     IEGMIYSVGKYKDRDTYLKKSYECTQELKDLVNEMIEVSKSEILEKDLKLVSINISEL
                     LNRLVKRQVFLIEEKHMKTILKIEENLEIKADQERITKAINNIINNAIKYSPEESELI
                     IRLYDKNKRVNKKNYNQRVVLEIENTGVTIEKRYLEEIFNPFYRIEKSRSRKTGGSGL
                     GLYIVSQIFKSHGFDYSIKNKENSVVFTVEFKN"
BASE COUNT         2116 a          528 c          896 g         1527 t
ORIGIN      
        1 taattatgag tatacttttc tattaaatac aaataaatat aactaataca tttatttatt
       61 aaaaaaatat tgatttaata tacctatagg gggtataata aaaatataga tttctaaata
      121 agtaatttat caatattaca tatcaaaata aaacgttaca aattagctaa aaatatattg
      181 aaagaaagga agattttatt atgagttcaa ataaagtggt agaaagttat aaaattacag
      241 gtatgacatg tgcagcttgt gcaaaggcag tagaaagagt aactaagaag atggatggag
      301 tatatgacca aagtgtaaat atagctactg aaaaacttaa aatagaatat gataattcta
      361 aggttaattt tgatgatata aagcaggttg tagaaaaagc aggatatgga attataaagg
      421 aagaaagtaa taaaaaaatt gatatgaaaa ttgatggaat gacatgtgca gcttgtgcaa
      481 aagctgtaga gagagtggta aaaaaattgg atggagtaga aagtattagt gtgaatattg
      541 caacagataa agctaatata gactatgacc catcaaaagt aaaattatct caaataaaag
      601 cagctattga aaaagctgga tataaaccaa tagaagaggt aagaaataag gttgatgtgg
      661 atgaagataa attgagaaaa gaaagagaaa tgaagtcttt atttgttaaa tttatagttg
      721 caatagtatt tgcagtacct ctgttttata tagcaatggg acctatgata ataaagccta
      781 tagggccatg gcctttgcca gaaattataa atcccatgac aaatactttt aattacgcat
      841 taattcagtt aattttagta ataccagtaa tgatagctgg ttataaattt tatataaatg
      901 ggtttaaatc acttttttct ttaagcccta atatggattc tttagttgca ataggaacat
      961 tagcagcatt tttatatagt ctatatacga cactacaaat atccaatgga caaattcaag
     1021 ggatgcacca tcatcagttg tattatgaaa gtgcaggaat aataatagca cttatcttac
     1081 ttggtaaata tttagaatca aaatcaaaag gaaaaacatc agaggcgatt aaaaagctta
     1141 tggggctaca acctaaaact gcaatagtgt tagttgatgg taaagaaatt gaaactccta
     1201 tagaagaagt agagatagga gatatactat tagtaaaacc aggaactaaa atacccgtgg
     1261 atggagttgt aatagaagga tatacatcag ttgatgaatc aatgcttaca ggtgaaagta
     1321 taccagtgga aaagaatgtt ggaagtaagg tgacaggagc tagtattaat aaaaatggtg
     1381 ttattaagtt taaggctgaa aaaattggtg gagatactgc tcttgctcaa ataataaagt
     1441 tagttgaaga tgcgcaaggt acaaaagcac ctattgctaa acttgctgat actgtatctg
     1501 gatattttgt accaatagta attgcaatag ctgtagttgc atcattattg tggtttttaa
     1561 taggaggaaa agatatagta tttgtactta ctatatttat atcagtactt gtaattgctt
     1621 gtccttgtgc attaggatta gcaacaccaa ctgctataat ggttgggact ggaaagggcg
     1681 cagaaaatgg aatcttgata aaaggtggag aggcactaga atctgcacat aaagtaaata
     1741 cagtgatatt tgataaaaca ggtactataa cagaaggaaa tccaaaagta acagatatag
     1801 tgttaaataa taatgtaaaa gaagaatatc ttatcaagat tgcttcaagt gcagaaaaag
     1861 gttcagaaca tcctcttgga gaagcaatag ttaagtatgg agaagagaag aatataaaat
     1921 ttgaaaaagt tgataatttt aaagctatac caggtgctgg tatacaagtt actattaatg
     1981 atgaaagtat attacttgga aatagaaagc ttatgaatga caataatata aagcttgggg
     2041 atttagaaga aaaatcaaat gtattggcaa gccaaggtaa aacacctatg tatatagctg
     2101 tagatggaaa tttatcaggt ataatagcag ttgcagatgt agtcaaagaa agtagtaaaa
     2161 aagcaataga aatacttcat gatatgggaa taaaagttgc aatggtaact ggagataatg
     2221 cgaaaactgc aaatgctata gcaaatcaag taggtataga tatggttttg gcagaggttt
     2281 taccagaaga taagtcaaaa gaagttgaaa aactacaaaa tcaaggcaag tttgttgcaa
     2341 tggtagggga tggaataaat gatgccccag ctcttgcaaa agcagatata gggatagcaa
     2401 taggaagtgg aacagatgta gccattgaat ctgcagatat agtacttatg aagagtgatt
     2461 taattgatgt tccaacagca attaaactta gtcatgaaac tataaaaaat ataaaacaaa
     2521 acctattttg ggcatttgga tataatacga ttggtatacc tgtagcagct ggaatattat
     2581 atgtatttgg aggaccttta ttaaacccaa tgattgcagc tgcagcaatg agcttaagtt
     2641 cagtttcagt cgtatcaaat gcacttagac ttaaaaactt caaggcatat aagagagatt
     2701 agattaataa attaaataaa atgtatcaaa aagagctatc tttaaattaa aaggattaat
     2761 ttacaaatta agatagctct tttttcatgt aaaattcaca acaataagat aaaatataag
     2821 taatatatga aattttaaat aaaatagaaa ggattgatag aatgaattcg tcaatacttg
     2881 taattgaaga tgattcaaat atacaagagt taatatcaga atttttaagt gcagaaggtt
     2941 atcaagttga tacagcaaat gatgggttgg aaggtataca aaagtttaag caaggaagtt
     3001 atgatttagt aatattagat ataatgatgc caaacttaga tggttatgga gtttgtaaaa
     3061 tgataagaaa gtcatcaagc gttccaatta tatttttaac tgctttaaat gatgaaggag
     3121 accaacttaa agggtttgac ttagaatgtg atgattatat aacaaaacca ttttcgttta
     3181 atcttcttat aaaaagagta gaagcaattt taaggcgaag taacaagact ataaatgata
     3241 aatttatagt ttttgaaaaa ttaaaattag atttaaacac atatattgca gagatagatg
     3301 gagaacctat agaacttaca ctaaaagagt ttaatatatt aaaagctttg atagaaaagt
     3361 atccacaagt tataactaga gaaggtcttt tggatagtat atggggttat gactattatg
     3421 gagatactag gattgttgat gcacatataa aaaatataag aaaaaaaata tcattaccat
     3481 atattaaaac tgtaaaagga atagggtata cacttgagaa ggatatttga taagtgggaa
     3541 aagttaagta taaagtataa gttattttca ataacgacat cattattaat agcattagct
     3601 ttaataatct atctaatttt atatttttta ttgccatcat attatcatga gtataaaatt
     3661 gagtctcttc aagaaagttt gaaatcttta gtagatagtt caattcattt cgatacatat
     3721 actttagaag aaagacttta ttatatggct aaagaccaaa atttggctat tttattaaaa
     3781 gatgaccaag ggaaaattgt atatggtaag aatgaagtag ttattttaag atatagtaag
     3841 tacatgatta atagtcttga ggatgagtat agaacttcta tacctatata tactaaagat
     3901 gcaaaagatg gtccatatac tttagaactc gttatgccat tacaaccaat tgacgaagca
     3961 aacgaagtta taagaaaact aatgccatac atcatatcaa tagcaatatt gatagcgatt
     4021 attggagcat atatatattc aattgtaata acaaagcctc ttataaatat aatagagagt
     4081 gagagagaac aggaatatag aaggaaggat tttgttgcta caatatcaca tgaactaaaa
     4141 actccaataa ccataataag tggtcaaatt gaaggtatga tttacagtgt aggtaagtat
     4201 aaggataggg atacttatct gaaaaaatcg tatgaatgta cacaagaatt aaaagattta
     4261 gtaaatgaaa tgatagaggt ttcaaaatca gaaatacttg aaaaagattt gaaattagta
     4321 tcaataaata taagtgaact attaaataga cttgtaaaaa gacaagtatt tttaatagaa
     4381 gaaaaacata tgaaaactat tttaaaaata gaagaaaatc tagaaattaa agcagaccaa
     4441 gagaggataa ctaaggcaat caataatata ataaataatg ctataaaata ttctcctgaa
     4501 gaatcggagc ttataattag actctatgat aaaaataaac gagtaaataa aaagaattat
     4561 aatcaaagag tagttttaga aattgaaaat actggtgtaa ctattgagaa acgttattta
     4621 gaagagatat ttaatccatt ttatagaata gaaaaatctc gctcaagaaa aactggagga
     4681 agtggactgg gactttatat agtcagccaa atttttaaaa gtcatgggtt tgattatagc
     4741 ataaaaaaca aagaaaactc tgtagtattc acagtagaat ttaaaaatta attacttatt
     4801 gtaaagaata tataactttc aataatagtt cattttaaaa tcacaaaaaa ttcataatga
     4861 aactgtatta tataaatata aagaagcttt aaaccataaa atacactagc tataatgatg
     4921 agatttcctt aaatcctcca tataagtcat cattatagta acccaacaat taagttacta
     4981 tgaatttaaa aaataacacc aagcatataa tctatgctta gtgttatttt tattttgaaa
     5041 taaaaaatat ttaatttatt gaaatat
//
LOCUS       DAEPXF010000233         5040 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain HB27
            SAMN13376389-rid12479423.denovo.241, whole genome shotgun sequence.
ACCESSION   DAEPXF010000233 DAEPXF010000000
VERSION     DAEPXF010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13376389
            Sequence Read Archive: SRR10518432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5040)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5040)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001069624.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001069624.1
            Genome Coverage       :: 315x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/15/2021 01:54:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,165
            CDSs (total)                      :: 4,076
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 89
            rRNAs                             :: 10, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 10 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 19 of 69
            Pseudo Genes (incomplete)         :: 44 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 10 of 69
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5040
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13376389-rid12479423.denovo.241"
                     /strain="HB27"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="China: Hebei"
                     /collection_date="2017"
     misc_binding    11..273
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            349..3015
                     /locus_tag="KRL67_003536"
     CDS             349..3015
                     /locus_tag="KRL67_003536"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HBH1524374.1"
                     /translation="MENTNLSKTYNPKDFEARLYKKWMDEGYFKSKPNPDKKPFTIMM
                     PPPNITGQLHMGHALDHTLQDILIRWKRMDGYEAFWLPGTDHASIATEVKVVERIKKQ
                     EGKTKYEIGREEFLKRAWEWKDEFGGKISNQLKQLGDSCDWDKERFTMDEGCNEAVIE
                     FFVSLYEKGHIYRGNRIINWCPDCKTTLSDAEVEHEEHDGNFYHIKYPLKDSEDFLEI
                     ATTRPETMIGDTGIAVNPEDDRYKHLIGKTAILPLVGRELPIVADSYVDLEFGTGAVK
                     MTPAHDPNDFEVGLRHNLEQLNTMNEDGTMNEVCGKYEGMDRFECRKAIVADLKEQGY
                     LIKIKEHNHNVGTCYRCHTVVEPRLSEQWFVKMEELAKPAIDILKKGELEFVPDKFDK
                     TYLQWLENIRDWCISRQLWWGHQIPAYYCQECGEIVVARKMPDKCPKCGSTHFKQDED
                     ALDTWFSSALWPFSTLGWPNKTEALDYYYPTSVLVTGYDIIFFWVVRMAFAGMFCMNE
                     KPFDHVLVHGLVRDSQGRKMSKSLGNGIDPLEIIEQYGADALRFTLTTGNSPGNDMRF
                     YMERVEFARNFANKLWNASRFVFMNIDEDIIKNMTRESVKEDLTLADKWIISRANNIV
                     KEATNNMDKFDLGIALQKIYDFTWSEYCDWYIEMVKPRLYGEDANAKSAALYTLTYVL
                     EKILKLLHPYMPFITEEIYTHLPTVEGCIIVSEWPKYNEEDNMAEEEDMMNLLMEGIR
                     SIRNVRAEMNVPPSKKAKLIIIPSEEKIEAIELGRDYFITLASASNVEIAKDKSNVPE
                     DAVGVVIDGVEIFIPLNELVDFEKEIERLSKEKKKLEGEIKRVNGKLANQGFLAKAPE
                     SLIEEEKAKKEKFEEMIKSVEERLANLESKIK"
     gene            3440..4150
                     /gene="rgaR"
                     /locus_tag="KRL67_003537"
     CDS             3440..4150
                     /gene="rgaR"
                     /locus_tag="KRL67_003537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RgaR"
                     /protein_id="HBH1524375.1"
                     /translation="MFRVVICDDEKIQRSILKEFTEKLLSERCLNYQILEFSCGEDLI
                     SKYPEKIDIIFLDIQMKDINGIEVAKKIRKFDEKVEIIFTTAFSEYAPQGYEVRAYRY
                     LVKPIEYSNFAMGVNLCIDNLQRKKERYIVLNSKKGFNRILIDSILYVETVKRDLVVH
                     TIGRDYKANMSMKQAEKLLCENGFFRCHTSFIVNLRRIEDLRDNMITINEQFIPISKY
                     RLKDLKVALTSLLCDIMY"
BASE COUNT         1990 a          501 c          890 g         1659 t
ORIGIN      
        1 atatgtaata ttaaagactg agaagaagag gagtaagtaa atactactaa aagagaggaa
       61 aattcactgg ctgtaagatt ttctaagtat aagatttact gaatgtagct ttggagtttc
      121 taaactgaac tatgagtaag tttagacggt tagaatcgtt aaattctttg agagtcaaag
      181 ttgataaaat aacattaact aataatttaa ttatatgtta atgtatttta agctttggaa
      241 ttaaggtggt aacgcgagct tttcgtcctt tttaaagagg atgaagagct cttttttatt
      301 tctaaagata tttaaaaaat ttatttataa aatacaggag gtaggacaat ggaaaatacg
      361 aatttatcaa aaacttataa cccaaaagat tttgaggcta ggttatataa aaaatggatg
      421 gatgaagggt attttaaatc taaaccaaat ccagataaaa aaccatttac aataatgatg
      481 ccaccaccaa atataacagg gcaattgcat atgggacacg ctcttgacca tacacttcaa
      541 gatatattaa tcagatggaa acgtatggat ggatatgaag ctttttggct tccaggaact
      601 gaccatgctt ctatagcaac agaagttaaa gttgttgaaa gaataaaaaa acaagaaggt
      661 aagacaaagt atgaaatagg tagagaagaa tttttaaaga gagcctggga atggaaagat
      721 gaatttggag ggaaaatatc taatcagctt aaacaactag gtgattcttg tgattgggat
      781 aaagaaagat ttactatgga tgaagggtgt aatgaagcag ttatagaatt ttttgtaagc
      841 ctatatgaaa aaggtcacat atatagagga aacagaataa taaactggtg tccagattgc
      901 aaaactactt tatcagatgc agaagttgaa catgaagaac acgacggaaa cttttatcat
      961 ataaagtacc cactaaaaga tagtgaagac tttttagaaa tagctacaac tagacctgag
     1021 acaatgatag gagatactgg tatagcagta aatccagaag atgatagata taaacattta
     1081 ataggaaaaa cagcaatact tccattagta ggaagagaac ttccaatagt tgcagatagt
     1141 tatgttgatt tagagtttgg aactggtgca gttaagatga ctccagcaca tgaccctaat
     1201 gattttgaag taggattaag acacaatctt gagcagttaa atactatgaa tgaagatgga
     1261 actatgaatg aagtttgtgg aaaatatgaa ggtatggata gatttgagtg tagaaaagcc
     1321 attgtagcag acttaaaaga acaaggttac ctaattaaga taaaagagca taatcataat
     1381 gtaggaactt gttatagatg ccatacagta gttgagccta gattatcaga gcaatggttt
     1441 gttaagatgg aagaacttgc aaaaccagct atagatattc ttaaaaaagg agaattagaa
     1501 tttgttccag ataaatttga taaaacttat ttacaatggc tagaaaatat aagagattgg
     1561 tgtatatcaa gacaattatg gtggggtcat caaataccag cttattattg tcaagagtgt
     1621 ggagaaatag tagttgctag aaaaatgcca gataagtgtc ctaaatgtgg aagcactcat
     1681 tttaaacaag atgaagatgc cttagatact tggttcagtt ctgcattatg gcctttctct
     1741 actctaggat ggccaaataa gactgaagct ttagattatt attatccaac aagtgtactt
     1801 gttacaggat acgatataat attcttctgg gtagttagaa tggcatttgc aggtatgttc
     1861 tgtatgaatg aaaagccatt tgaccatgtt ttggttcatg gattagtaag agattctcaa
     1921 ggtagaaaaa tgagtaaatc tttaggaaat ggtatcgacc cattggagat aatcgagcaa
     1981 tatggagctg atgccctaag atttacatta acaactggaa attccccagg aaatgatatg
     2041 agattctata tggaaagagt agaatttgct agaaactttg caaataagtt gtggaatgca
     2101 tcaagatttg tatttatgaa tattgatgaa gatataatta aaaacatgac tagagaaagt
     2161 gttaaagagg acttaactct agctgataaa tggataatat caagagcaaa taatattgta
     2221 aaagaagcaa ctaataacat ggataagttt gatttaggaa tagcacttca aaagatatat
     2281 gattttactt ggtcagaata ctgtgactgg tatattgaaa tggttaaacc tagattatat
     2341 ggggaagatg caaatgctaa atctgcagca ttatatacat taacttatgt tcttgaaaag
     2401 atattaaaat tattacatcc atacatgcca tttataacag aggaaatata tactcatctt
     2461 cctactgttg aaggatgtat aatagtatct gaatggccaa aatataatga agaagataac
     2521 atggctgaag aagaagatat gatgaactta ttgatggaag gtataagaag tataagaaat
     2581 gtgagagcag aaatgaatgt accaccttct aagaaagcta aattgataat aatcccaagt
     2641 gaagaaaaaa tagaagctat tgagcttggc agagactatt ttataacttt agcatctgct
     2701 tcaaatgtag agatagcaaa agataaatct aatgttccag aagatgcagt tggagtagtt
     2761 attgatggtg ttgaaatatt tatacctctt aatgaattag ttgattttga aaaagaaatt
     2821 gagagattat ctaaagaaaa gaaaaagtta gaaggcgaaa taaagagagt taatggaaag
     2881 cttgcaaatc aaggtttctt agcaaaagct cctgaaagtt taattgaaga agaaaaagca
     2941 aaaaaagaaa aatttgaaga gatgattaaa tctgttgagg aaagattagc aaatttagaa
     3001 tctaaaataa aataaatata agaatgagta aaaacataca aaaagtatat attctatact
     3061 ttttgtatgt ttttctttat atatatacaa atatgatgtt ataattcttt acagatgtgt
     3121 tatattttta ttttatgttg tgaatatatg aattttatgt cttaaaattt ataagcaata
     3181 aaaatttgat atattatgat gtttaatata gggtaataaa tacatattat atttatttta
     3241 ctatattttt ttacataata tatctagaaa agtataggaa tttatcgtaa tttaagttta
     3301 aatattaaag agaatttcta taagtagttt aatcgacaat ttaaatttat aaagaaattt
     3361 aaatttgaaa gctagtgagt acagtttgga aattgaaata aagtaaacta aatgatattt
     3421 tagatttgga ggtgatatta tgttcagggt agttatatgt gatgacgaaa aaatacaaag
     3481 aagcatacta aaagagttta cagaaaaact tttgtctgaa agatgtctaa attatcaaat
     3541 actggaattt tcctgtggag aagatttaat atccaaatac ccagaaaaaa ttgatattat
     3601 atttttagat atccaaatga aagatatcaa tggaattgag gtggctaaaa aaataagaaa
     3661 gtttgatgaa aaagtagaaa ttatatttac aacagctttt tcagagtatg ccccccaagg
     3721 ctatgaggtt cgtgcttata gatacttagt aaagcctata gagtatagta actttgcaat
     3781 gggtgtaaat ctttgtattg ataaccttca aagaaagaag gagcgttata tagttttaaa
     3841 tagtaaaaaa ggatttaata gaatactgat agattctatt ttatatgttg aaactgtaaa
     3901 gagagattta gttgtacata caataggtag agattataaa gctaatatga gtatgaaaca
     3961 agcagaaaaa ttactgtgtg aaaatggctt ttttagatgt catacaagtt ttattgttaa
     4021 tttaagaaga atagaagatc ttagggataa tatgattact attaatgaac aatttatacc
     4081 tataagtaag tatagactta aagatttaaa agtagcatta acaagcttgt tatgtgatat
     4141 aatgtattaa ttttaaaaag gtaattgaaa atatcgtaaa aaagatttaa tgtcatcaca
     4201 gggctattga ttaaaactgt cttaatatat aaaatgttat ttatattaag acagttttat
     4261 tatgcaaaat agcagtttat agacttttaa tattgtttaa tatgtatatg tagaggttcc
     4321 attatagtag cttgactatc aattagtagc ttgattatca atatactatt attgattttt
     4381 atatgcagta tatgtatttt tcgaatatga ttaccttttt aaaataatga ctatttataa
     4441 aactctatag atatttgtct catttagcta tgcatacttt aattaattat attaatattt
     4501 aatggaggac aatataaata ttaaagctat atcttatagt ttattgaacc tatagtaatg
     4561 aattaataaa tagtttaatt ctatgaaaag atttcctttt atgcaattta tttattgaaa
     4621 tatgatataa tatatgaagg tgtaagtatt agttgttaaa ataatattta tattagtttt
     4681 tatgtatact tggtgtaagt ttttcatata tcgattaaaa ttgatgttga tttattattt
     4741 ttagtatttt ttcacctttt gattcatact ttgtagaatg gtgtattttc taaaactggt
     4801 tataagacaa gttcgttcaa attagcattt ttatagcata taaataattg ttccccctaa
     4861 ataagattta gatgtatagt attaaaatca aaaagtataa aataaaattg atgcttaatc
     4921 atagataaat attaataaaa tatacaaggg gattgtctaa taaaaaagat aatctccttt
     4981 tttacaaata attctaaatt gtgatataaa tctaaaaatt ttatcttaac atttaaccat
//
LOCUS       DAEPXF010000234         4978 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain HB27
            SAMN13376389-rid12479423.denovo.242, whole genome shotgun sequence.
ACCESSION   DAEPXF010000234 DAEPXF010000000
VERSION     DAEPXF010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13376389
            Sequence Read Archive: SRR10518432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4978)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4978)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001069624.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001069624.1
            Genome Coverage       :: 315x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/15/2021 01:54:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,165
            CDSs (total)                      :: 4,076
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 89
            rRNAs                             :: 10, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 10 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 19 of 69
            Pseudo Genes (incomplete)         :: 44 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 10 of 69
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4978
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13376389-rid12479423.denovo.242"
                     /strain="HB27"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="China: Hebei"
                     /collection_date="2017"
     gene            136..636
                     /locus_tag="KRL67_003538"
     CDS             136..636
                     /locus_tag="KRL67_003538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HBH1524376.1"
                     /translation="MRKNSINIALTAAITAIYAVLTLSLGFISYGPIQFRVAEIMMLL
                     AFLDKGYIVGLTLGCFLANVIGPYGVPDIIFGTLATFISASMIYITRKLGKQNKSTLI
                     IASLWPTIINSIVVGLMLNVFFGLPLILSMIQVGFGEFVVVTIVGVPFFCFLMKKYKN
                     VINIKF"
     gene            693..1679
                     /locus_tag="KRL67_003539"
     CDS             693..1679
                     /locus_tag="KRL67_003539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WYL domain-containing protein"
                     /protein_id="HBH1524377.1"
                     /translation="MSKVKRLTDVWTYINDKQKFTVKELSEEFNLSAKTIQRYLKELN
                     KMGLPIQAEQGRNGGYRVLNNSYIPPVIFTEKEVMTILFALKSLQIYNYKLIEIEINS
                     IMRKISYERKSSIKVGIENIKRYIGFVANDTNDGDFKSINVNEFFKASSESLILNIKY
                     KFGNQILEKNICPIGIYLNKGAWFSPAYDCKSDKITLVHIDNIIDIKRTGESKKIHIN
                     LEEWLYEVYNNLYGNSSTTKKIGETIYLNVLLTKEGVSKIKDSSWNLDMEINEDGSGI
                     ISTFIKVEDIDCYASILFLIGNNAKVIEPKFLNKLLYDKANELRYFYEENMV"
     gene            1729..2451
                     /locus_tag="KRL67_003540"
     CDS             1729..2451
                     /locus_tag="KRL67_003540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein"
                     /protein_id="HBH1524378.1"
                     /translation="MENFNKNAKIIGIGAEGINIINEVEEKIKANMDIEKININQEIE
                     KEYVRSLLDGVDILFLIYSSEDKHIRDIIKAVSYMSNERRVLSIGMDCSEKENKEDLE
                     LGREFKINNDSIFKFVDLMNIMVESISDSCMINIDITDLKEAIVGDKGIKYSFEEFED
                     TKSYSEMADILFDRMEYIGEEFISKKGIVFVEGSPEFSIMELNDLINNIQSKVEESYE
                     VIFSLYMKENLNGNIRVGLLYN"
     gene            2480..4414
                     /locus_tag="KRL67_003541"
     CDS             2480..4414
                     /locus_tag="KRL67_003541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBH1524379.1"
                     /translation="MSENIRGTEYWLMEEFFYQETDTPQIFREESIIALGFDIKINLK
                     KVFNMGKKSEKYINENCNDKDFIKKFMSIKKGDYFLYKAYQNIEEKVKCNVSIGIVEE
                     NFENGYELSHILGHTLPVKWMHNFDTVSNCKVYTSEFYKLNNNDISSYLEIIKSQDKK
                     SLRKRIEESENNKWYLKNYYYKNVDKCVDDEELFIQPINLEQLENLKNIKTDDYILLY
                     NSEKVICENDHTMKSLFGVCQVMEGFDKEKVSTLEGGYFFPVRWIEYEKEHIDGNAWV
                     IFENNSIVEKYIKRFTNKMPLNTILYGPPGTGKTYNTKQMVYNLVSETSLRNRYYTGY
                     LDYKNNVEFCTFHQSYGYEEFIEGLKSDGEGNFKPEDGILKDISIEACYNALRFEKKM
                     SFELEKDSLTLETIKERKKKLVLENIEYSESFNFEESENYVLIIDEINRGNVSKIFGE
                     LITLLEDDKRLTKSNQTIVKLPYSKEKFSLPPNLYIVGTMNTSDKSIALLDIALRRRF
                     NFEEIMPDYDLLSIVDGIDVRKMLYTINKRIEFLYDRDHVIGQAYLLAVENINDIVVV
                     FRSKIIPLLQEYFYYDSEKVGLILGGIGRNEKDKYIVYKEKLIASELFKNNNASIMES
                     KVNYCIKQNISSEELKNIYE"
     gene            4407..>4978
                     /locus_tag="KRL67_003542"
     CDS             4407..>4978
                     /locus_tag="KRL67_003542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH1524380.1"
                     /translation="MSKNIYVKETYEWIRVGNGENELTEIEYEKLLKYLENNNDVLKS
                     NIVDIKYKKLRFINYVGIICFENVILEILPKLSLSDNLVKDREILLQMLSICNKIPII
                     MNEKIRLSLKNYNLLNFFVMYFIESMQTQMKRGIYFEYINKIENLNVIRGKILLSTYA
                     KEKGISPMKIRCEYDEYSENNFLNQVLKKA"
BASE COUNT         2082 a          379 c          867 g         1650 t
ORIGIN      
        1 ataaaatgaa ttaggaaaat agttcgttaa aataatttgt ttttataaaa ttactgttta
       61 agtttataga ttttgtataa aataaagtta tgttattaat ttttttagaa ataaaacttt
      121 tattaggaga cagatatgag aaaaaatagt attaatatag cactaactgc agcaattaca
      181 gcaatctatg cagttttaac attatcattg ggatttataa gttatggacc aatacaattt
      241 agggtagcag agattatgat gcttttggca tttttagata aaggatatat agtaggtctt
      301 acattaggtt gttttttagc aaatgtaata ggaccatatg gtgtacctga tataattttt
      361 ggaacattag ccacatttat aagtgcaagt atgatttata ttactagaaa gttaggtaaa
      421 caaaataaaa gtactttaat tatagcttct ttatggccaa ctattataaa ttcgattgtg
      481 gttggactaa tgttaaatgt attctttgga cttccattaa ttctatccat gatacaagta
      541 ggttttggag aatttgtagt agtaacaatt gtaggagtac catttttttg ctttttaatg
      601 aaaaaatata aaaatgtcat taatattaaa ttttaaatag tgtatagtaa tttaattaat
      661 tgagagtatt attagtgaaa agaggtgttt atttgtcaaa ggtaaaaaga ttgactgatg
      721 tttggacata tattaatgat aagcaaaagt ttacagtaaa agagttgtca gaggaattta
      781 atctttcagc taaaacaata caacgatatt taaaagaatt aaataaaatg ggacttccaa
      841 ttcaagcaga acaaggtaga aatggaggtt atagggtgct aaataatagc tatatacctc
      901 ctgttatatt tacagaaaaa gaagttatga ctatactatt tgcacttaag tcacttcaga
      961 tttataatta taagttaata gaaatagaaa ttaattctat aatgagaaaa ataagttatg
     1021 agcgtaagag cagcataaaa gtaggtatag aaaatattaa aagatacatt ggctttgtag
     1081 ctaatgatac taatgatgga gattttaaga gtatcaatgt aaatgagttt tttaaagctt
     1141 cttccgaatc tctaatttta aatattaagt ataagtttgg taatcagatt ttggaaaaaa
     1201 acatatgtcc tataggcata tatttaaata aaggagcatg gttttcacca gcctatgatt
     1261 gtaaaagtga taaaataaca ttagttcata tagataatat aattgatata aaaagaacag
     1321 gagaaagtaa aaaaatacat ataaacttgg aagaatggtt atatgaagta tataacaatt
     1381 tatatggaaa tagttcaact actaaaaaaa ttggtgaaac tatttatcta aatgtattat
     1441 taactaaaga aggcgtttct aaaataaagg acagttcttg gaatttagat atggagataa
     1501 atgaggatgg tagtggaata attagtacat tcataaaggt tgaagatata gattgttatg
     1561 cttctatttt atttttaatt ggaaataatg ctaaggttat agagccaaaa tttctgaata
     1621 aactacttta tgataaggcc aatgagttaa gatattttta tgaagaaaat atggtataat
     1681 atttagaaat gttgaaatta aataattatt aaggagatat acgttataat ggaaaatttt
     1741 aataagaatg ctaaaataat tggtattgga gctgaaggta ttaatataat aaatgaagta
     1801 gaagaaaaaa taaaagcaaa tatggatata gaaaaaataa acataaatca agagattgaa
     1861 aaggaatatg ttagaagtct tttagatggc gtagatatat tatttttgat atattcatct
     1921 gaagataaac atattagaga tattataaaa gctgtttcat atatgtcaaa cgaaagaaga
     1981 gtcctatcta taggaatgga ttgctcagag aaagaaaata aagaagattt agagcttggg
     2041 agagagttta aaatcaataa tgatagtata tttaaatttg ttgatttaat gaatatcatg
     2101 gtagagtcta tttctgattc ttgcatgata aatatagata ttactgattt aaaagaagct
     2161 attgtaggag ataaaggaat taagtattct tttgaagagt ttgaagatac aaagtcatat
     2221 agcgagatgg ctgacatttt atttgacagg atggaatata ttggtgagga atttataagt
     2281 aagaaaggaa ttgtatttgt tgaagggagt ccagaatttt ctataatgga attgaatgat
     2341 ttaataaata atattcagag caaggtagaa gaaagttatg aagtaatatt ttctttatat
     2401 atgaaggaaa atttaaatgg aaatattaga gtaggtttat tgtataatta gtatatttta
     2461 gatattaagg agagtgtata tgtctgaaaa tataagaggt acagagtatt ggctaatgga
     2521 agaatttttt tatcaggaaa cagatacacc acagattttt agagaagaaa gtataattgc
     2581 acttggattt gatataaaaa taaatttaaa aaaagtattc aatatgggaa aaaaatcaga
     2641 aaagtatatt aatgagaatt gtaatgataa agattttata aaaaagttta tgtcgattaa
     2701 aaaaggagat tattttttat ataaagcata tcagaatata gaagaaaaag ttaaatgtaa
     2761 tgtgtctatt ggcatagttg aagaaaattt tgaaaatgga tatgaattaa gtcatatttt
     2821 agggcataca ctaccagtaa aatggatgca taattttgat acagtttcaa attgtaaagt
     2881 atatacaagt gaattttata aattaaataa taatgatatt agctcatatt tagaaattat
     2941 taagtcacaa gataagaaat cattgagaaa aagaatagaa gaaagtgaaa ataataaatg
     3001 gtatttaaaa aactattatt ataaaaatgt agataagtgt gtagatgatg aagaattatt
     3061 tattcagccc attaatctag aacaattaga aaacctaaaa aatataaaga cagatgatta
     3121 tattttattg tataatagcg agaaagtaat ttgtgaaaat gaccatacaa tgaagagtct
     3181 atttggcgta tgtcaagtta tggaagggtt cgataaagag aaagtaagta ctttggaggg
     3241 tggatatttt tttcctgtta gatggataga gtatgaaaaa gagcatatag acggaaatgc
     3301 atgggttata tttgaaaata atagtatagt tgaaaagtat ataaaaagat ttactaataa
     3361 aatgccatta aatactatac tttatggacc accaggaact ggtaagacat acaatacaaa
     3421 acagatggtt tacaatctag taagtgaaac tagtctaaga aataggtact atacaggtta
     3481 cttagattat aaaaacaatg tagaattttg tacatttcat caatcatatg gatatgagga
     3541 atttatagag ggccttaagt ctgatggtga aggcaatttt aaaccagaag atgggatatt
     3601 aaaggatata tctatagaag catgttataa tgcattgaga tttgaaaaaa agatgagttt
     3661 tgagttggaa aaagatagct tgactttaga aactataaaa gagaggaaaa agaaattagt
     3721 attagaaaat atagaatata gtgagagttt taattttgaa gaatcagaaa attatgtttt
     3781 aataatagat gaaattaata gaggaaatgt atctaaaata tttggtgagc taataacatt
     3841 attggaagat gataaaagac ttactaaatc aaatcaaact attgtcaaac taccatattc
     3901 aaaagaaaaa ttttctctac ctccaaattt gtatatagtt ggaactatga acacatcaga
     3961 taaatcaata gcacttttag atatagcttt aagaagaagg tttaattttg aagaaattat
     4021 gccagattat gatttattgt ctattgtaga tggtatagat gttagaaaaa tgctatatac
     4081 aatcaataaa aggatagagt ttttatatga tagagaccat gttattggac aagcttatct
     4141 acttgctgta gaaaatatta atgatatagt agtagtattt agaagtaaaa taataccact
     4201 tcttcaagag tatttttatt atgattcaga aaaagtaggt ttgattttag gaggaattgg
     4261 aagaaatgaa aaagataaat acattgttta taaagaaaaa cttatagctt ctgagttatt
     4321 taaaaataat aatgcttcaa taatggagag taaagttaac tattgtataa aacaaaacat
     4381 atctagtgag gagctaaaaa atatatatga gtaaaaatat atatgttaag gaaacctatg
     4441 aatggataag agtaggtaat ggtgaaaatg agcttacaga aattgaatat gaaaaattgc
     4501 ttaaatatct agagaacaat aatgatgtat taaaaagtaa tatagtagat attaaataca
     4561 aaaaactcag atttataaac tatgtaggca ttatatgttt tgaaaatgta atacttgaaa
     4621 tacttcctaa attgtcacta agtgataatc ttgtaaaaga tagagagata ctattacaga
     4681 tgctttctat atgtaataaa attccaatta taatgaatga aaaaataaga ttgagtttga
     4741 aaaattataa tctcttaaat ttttttgtta tgtatttcat tgagagtatg caaacacaga
     4801 tgaagagagg gatttacttt gaatatataa ataaaataga gaatttaaat gttattaggg
     4861 gtaaaatatt attaagtaca tatgcaaaag aaaaaggcat ttctccaatg aaaataagat
     4921 gtgaatatga tgagtatagt gaaaataatt tcttgaatca agttcttaaa aaagcatg
//
LOCUS       DAEPXF010000235         4822 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain HB27
            SAMN13376389-rid12479423.denovo.243, whole genome shotgun sequence.
ACCESSION   DAEPXF010000235 DAEPXF010000000
VERSION     DAEPXF010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13376389
            Sequence Read Archive: SRR10518432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4822)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4822)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001069624.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001069624.1
            Genome Coverage       :: 315x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/15/2021 01:54:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,165
            CDSs (total)                      :: 4,076
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 89
            rRNAs                             :: 10, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 10 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 19 of 69
            Pseudo Genes (incomplete)         :: 44 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 10 of 69
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4822
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13376389-rid12479423.denovo.243"
                     /strain="HB27"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="China: Hebei"
                     /collection_date="2017"
     gene            complement(<1..171)
                     /locus_tag="KRL67_003543"
     CDS             complement(<1..171)
                     /locus_tag="KRL67_003543"
                     /inference="COORDINATES: protein motif:HMM:NF013677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBH1524381.1"
                     /translation="MSKKHKKYSKELKLKAVNLYIKEGYSSYKIAEMLNIRSKTQVQN
                     WVKDYKNKGKTAF"
     gene            complement(315..905)
                     /locus_tag="KRL67_003544"
     CDS             complement(315..905)
                     /locus_tag="KRL67_003544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipicolinate synthase subunit B"
                     /protein_id="HBH1524382.1"
                     /translation="MRLEGLTIGVGFTGSFCTYDKIFIELENLVKEGANVHTIFSDVS
                     QNIDCRFGNSEEFMKKAYELTGNKPIVTIEEAEPFGPKGIADIIIIAPCTGNTAAKLA
                     NGITDSPVLMAAKGHLRNDKPLVISISTNDALSFNFKNIGILLNSKNIYFVPFGQDNC
                     KAKPNSMIAHTELIIPTIELALENKQLQPVIKSPHQ"
     gene            complement(908..1783)
                     /gene="dpsA"
                     /locus_tag="KRL67_003545"
     CDS             complement(908..1783)
                     /gene="dpsA"
                     /locus_tag="KRL67_003545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipicolinate synthase subunit DpsA"
                     /protein_id="HBH1524383.1"
                     /translation="MSNQYDITVIGGDLRQVYMVKKLINKGYSVVTYGISNEIINGIV
                     GKSTSLAEALSKSSIILCPIPFTKNQVNIENTDDCLDATIDNLLINLSPNQILFGGNI
                     PTKVCKFCEQHSIQIYDFMKMNDVAILNAVATAEGAIAEAIKRSIINIHQSNCLVLGY
                     GRCAQILAKKLYALDANVCVGARNNDARSTANAFGYSSISLNQLDEKLLQFDYIFNTI
                     PECILTKERLEKVSQEVTIIDIASSPGGVDYSVVKDAGINATLCLGLPGKYSPKTSGE
                     ILVNAIENIINERRD"
     gene            complement(1859..3250)
                     /locus_tag="KRL67_003546"
     CDS             complement(1859..3250)
                     /locus_tag="KRL67_003546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HBH1524384.1"
                     /translation="MNYYELLRIKKERHKNKKFLIIDGEKYTYENILSYSEELGKQIS
                     LGENIPILIYSKNIMFQLISFFAINYSKNIPIICHYNLSKKVLDDILLKNNISIIISD
                     ESMDVIDLYDDNNDEVVKKVNFSIFNPKFNIYMYQYKDLINYLDKSICMGALSSGSTS
                     VPKVLYRTYESWAGFFPIQNGIFNISGESILFVNGTLSFTGNLNSIMSVLYEGGSIVI
                     SSGLNCKSWIRTIKQYNVTNIYLIPTKLQLLVKHLKEPVLKVVSIFTGSQLLFEDTAR
                     NLKKYMPKSEIILYYGASELNYITYLCYDELIEKPLSVGRPFPGIDIYIRKGKIFVNT
                     EYAVYNATKPYSVNDIGYFDNDGYLIFEGRSDDIVNIGGFKVSSTKIENEVKKIPQIE
                     DAVVLPYSDTIRGNQIVLFVITIDKITKKDLLIKMKDNLMKNEIPKKIIFLNSFPYTS
                     SEKIDRLALLKML"
     gene            complement(3254..4390)
                     /locus_tag="KRL67_003547"
     CDS             complement(3254..4390)
                     /locus_tag="KRL67_003547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HBH1524385.1"
                     /translation="MKEVFILGGLRSHIGLKNGIFQFVQPELLGASVLKDLIKKYEID
                     KIDEIICGNAVGTGGNIARLMTLTAGVSNEVPAFTVDMQCASAMMSIDIAFSKVKSGQ
                     CDLIIAGGFESSSLQPMRTYHKNDKRYNTNNPNYTVAQFSPDDNSQNSMLEGAERVAE
                     LYQIEKADLDFWVKESHKRAKEAREEKILEDIISPINNSTYDEGIRDKMSQRLLDRMP
                     SILGKETITNAANSCLINDGASFIIICSKKYLEHVKKKPKAKIINTCTIGTDATLSPT
                     SAIQAMDKLLEIESLNYLDVSAVEFNEAFAVIDVLFQRKYPELIDRYNIFGGALAYGH
                     PYGASGAIIALHLLKALEKTKGRYGICSIAAAGGLGSALLLERV"
     gene            complement(4422..>4822)
                     /locus_tag="KRL67_003548"
     CDS             complement(4422..>4822)
                     /locus_tag="KRL67_003548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HBH1524386.1"
                     /translation="LILRPRQAFSTVGVYIMIGLIGIPVFGGGTSGIGKLLSPTGGFY
                     FGFLFAALIISLLKGRNNDIKRYILITIFVGMPIIYFMGTVFMCYFNQMTVEAALFAA
                     VVPFLIGDTIKSVIASFLGTKLNNVLRRNL"
BASE COUNT         1587 a          837 c          560 g         1838 t
ORIGIN      
        1 aaatgcagtt ttacctttat ttttataatc ttttacccag ttttgaactt gtgttttgct
       61 tcttatattc agcatctcag caattttgta tgatgagtat ccttctttta tatataagtt
      121 tacagctttt agcttaagtt ctttgctata ttttttgtgt tttttagaca tagaaaaacc
      181 cccttagagt atacgctttt tagacctatt ataatagcct tttatttaaa tgtctactat
      241 aaggggatta tactaatttt gagacagcct ctttgctagt attgcatcta atacataata
      301 ttatagaacc aattctattg atgtggactt tttataacag gctgtagctg tttattctct
      361 agagcaagtt ctatagttgg aattattagc tcagtatgtg caatcataga attaggcttt
      421 gctttgcaat tatcttgacc aaaaggtaca aaatatatat ttttagaatt aaggagaata
      481 ccaatatttt tgaaattaaa gcttagagca tcattcgttg atattgatat tactagtggt
      541 ttatcattac gaagatgacc ttttgctgcc attaatactg gtgaatctgt aataccattt
      601 gctaattttg cagcagtatt tccagtacaa ggagctatta ttataatatc agcgattcct
      661 ttaggtccaa atggctctgc ctcttcaatc gtaactatag gtttatttcc tgtaagttca
      721 tatgcttttt tcataaactc ttcagaattt ccaaatctac aatcgatatt ttgtgataca
      781 tctgaaaata tagtgtgtac attagctcct tctttaacca aattctctaa ctctataaat
      841 attttatcat aagtacaaaa cgaaccagta aatcctactc caattgtaag tccttcaagt
      901 ctcatttcta atcccttctt tcatttataa tattttctat agcgttaaca agaatttcac
      961 cagatgtctt tggtgaatat ttaccaggaa gcccaaggca aagagtcgca ttaatccctg
     1021 cgtctttaac aacagaataa tctactcctc caggagaaga agctatatcg ataatagtta
     1081 cttcttgtga tactttttct aatctttctt tcgttaaaat acactctggt atggtgttaa
     1141 aaatataatc aaattgtaaa agtttttcat caagttgatt taaagaaata ctggaatatc
     1201 caaatgcatt agccgtactt cttgcatcat tatttcttgc accaacacat acatttgcat
     1261 ccaaagcgta taattttttt gcaagtattt gtgcacatct tccataacct aatactaaac
     1321 agttactttg atgtatattg attatacttc ttttaatagc ttctgctata gccccttcag
     1381 ctgtagcaac tgcatttaaa atagcaacat cattcatttt cataaaatca taaatctgaa
     1441 tagagtgttg ctcgcaaaat ttacaaactt tagttggtat gttacctcca aataatattt
     1501 gattaggaga taaattaatt agcaagttat caatagtagc atcaagacaa tcatcagtat
     1561 tttcaatatt aacttgattc tttgtaaatg gaattggaca taaaataatg gaacttttgg
     1621 atagtgcttc agctagtgaa gttgattttc caacgattcc atttataatt tcatttgata
     1681 tcccataagt gactacagaa taccctttat taattagttt ttttaccatg tacacttgtc
     1741 gcaggtctcc cccaataaca gttatatcgt attgatttga catattgtaa gcctccttta
     1801 ctatatacaa tatgttttta tcaatacatt ggttcactaa aattactaaa tattatactt
     1861 ataacatttt taataatgct aatctatcta tcttttcaga agaggtatat ggaaatgaat
     1921 ttaaaaagat aattttcttt gggatttcat ttttcattag attatctttc attttaatca
     1981 ataaatcttt ctttgtaatt ttgtctatag ttattacaaa taatacaatt tgatttcctc
     2041 ttatagtgtc tgaatacggc aatacaactg catcttcaat ttgaggaatt tttttaactt
     2101 cattttcaat cttagtagaa ctaactttaa atcctccaat atttacaatg tcatcacttc
     2161 ttccttcaaa tattaaatat ccatcattat caaaatatcc aatatcattg acagaataag
     2221 gtttagttgc gttatatact gcatattctg tattcacaaa aatttttcct tttctaatgt
     2281 atatatctat tcctggaaat ggtctgccta cacttagagg tttttcaatt agttcatcat
     2341 agcataaata tgtaatataa tttaattcac ttgccccata gtataaaata atttcagact
     2401 ttggcatata tttttttaga tttcttgcag tatcttcaaa taataattga gaccctgtaa
     2461 aaatacttac aactttgagt actggttctt ttaagtgttt aaccaaaagt tgaagttttg
     2521 taggtataag gtatatgtta gttacattat attgttttat tgtcctgatc catgatttac
     2581 aattaagacc tgaacttatt acaatactac ctccttcata taatactgac attatagaat
     2641 ttagattccc tgtaaaacta agtgttccat taacaaacaa gattgattct ccagagatat
     2701 taaaaatgcc attttgtata ggaaaaaatc cagcccaact ctcatatgtt ctatataata
     2761 cttttggaac acttgtagaa cctgaagata aagctcccat acaaatactt ttatctaaat
     2821 aatttataag atccttatat tgatacatat atatattgaa tttaggatta aaaatagaaa
     2881 aattaacttt ttttactacc tcatcattgt tatcatcata taaatcaata acgtccatag
     2941 attcatcaga tataatgatt gaaatattat tttttagtaa aatatcatct aatacttttt
     3001 tagaaagatt ataatgacaa ataataggaa tattttttga ataattaata gcaaaaaaac
     3061 taatcaactg aaacattata tttttagaat atattaaaat tgggatattt tcacctaaag
     3121 aaatttgttt tcctagttcc tcactataac tcaatatatt ttcataagta tatttttctc
     3181 catctataat taaaaatttt ttatttttat gcctctcttt ttttattctt aaaagttcat
     3241 aatagttcat aatctacacc ctttcaagca gtaaagctga acctagacca ccagctgcag
     3301 ctatagagca aattccatat ctacccttag tcttttctaa tgcttttaaa agatgtaatg
     3361 caataattgc acctgaagca ccatatggat gtccatatgc taaagctcca ccaaatatat
     3421 tatatctatc tattaactct ggatactttc tttgaaacaa cacatctata actgcaaatg
     3481 cttcattaaa ttcaacagca gatacatcta aataattaag gctttctatc tctaaaagtt
     3541 tatccattgc ttgtatcgct gatgttggac ttaaggttgc atctgttcct attgtgcatg
     3601 tattaataat ttttgcttta ggcttctttt ttacatgttc taaatacttc ttagaacata
     3661 ttataataaa tgaagctcca tcattaataa gacatgaatt tgctgcattt gtaatagttt
     3721 cttttcctaa aattgatggc atcctatcta ataacctttg actcatctta tcacgtatac
     3781 cttcatcata ggtactatta tttataggag atataatatc ctcaagtatt ttttcttctc
     3841 ttgcttcttt tgctctttta tgactttcct ttacccaaaa atccaagtct gctttctcaa
     3901 tttgatataa ctctgctact ctttccgccc cttcgagcat actattttgg ctattatcat
     3961 caggagaaaa ttgagcaact gtataatttg gattatttgt attatatcgc ttatcattct
     4021 tatgatatgt gcgcattggt tgtagagaac tactttcaaa accacctgca ataattaaat
     4081 cacactgacc agatttaacc tttgaaaaag ctatgtctat actcatcata gcagaagcac
     4141 actgcatatc cacagtaaaa gcaggtactt catttgacac tcctgctgtt aaagtcatta
     4201 atctagctat attccctcct gtcccaactg catttccaca tataatctca tcaattttgt
     4261 caatttcata ctttttaatt aaatctttta aaacacttgc tcctagtaat tcaggttgaa
     4321 caaattgaaa aattccattt tttagaccta tatgacttct caatcctcct aatataaaaa
     4381 cctccttcat acattacacc cttccttata ttatagattt atcatagatt tcttctgagt
     4441 acattgttca atttagtacc taaaaaagat gcaataactg actttattgt atctccaatt
     4501 aaaaatggta ctactgctgc aaaaagtgct gcttctactg tcatttgatt aaaatagcac
     4561 ataaatactg ttcccataaa atatataata ggcattccaa caaaaattgt tattagtata
     4621 tatctcttaa tatcattatt tcgtcctttt aataaactaa taattagtgc tgcaaacaaa
     4681 aaaccaaaat aaaaaccacc tgttgggctt aacaactttc caataccaga tgtacctcca
     4741 ccaaatactg gaatcccaat taaacctatc attatatata caccaactgt actaaatgct
     4801 tgtcttggtc ttaaaatcaa ag
//
LOCUS       DAEPXF010000236         4786 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain HB27
            SAMN13376389-rid12479423.denovo.244, whole genome shotgun sequence.
ACCESSION   DAEPXF010000236 DAEPXF010000000
VERSION     DAEPXF010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13376389
            Sequence Read Archive: SRR10518432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4786)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4786)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001069624.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001069624.1
            Genome Coverage       :: 315x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/15/2021 01:54:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,165
            CDSs (total)                      :: 4,076
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 89
            rRNAs                             :: 10, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 10 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 19 of 69
            Pseudo Genes (incomplete)         :: 44 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 10 of 69
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4786
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13376389-rid12479423.denovo.244"
                     /strain="HB27"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="China: Hebei"
                     /collection_date="2017"
     gene            complement(1..556)
                     /locus_tag="KRL67_003549"
                     /pseudo
     CDS             complement(1..556)
                     /locus_tag="KRL67_003549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861600.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            complement(558..665)
                     /locus_tag="KRL67_003550"
     CDS             complement(558..665)
                     /locus_tag="KRL67_003550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013483530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBH1524387.1"
                     /translation="MGRHLWNPSYFVATVSENTEEQIRKYINNQKKQVK"
     gene            complement(1297..2454)
                     /locus_tag="KRL67_003551"
                     /pseudo
     CDS             complement(1297..2454)
                     /locus_tag="KRL67_003551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729454.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
     gene            complement(2497..2892)
                     /locus_tag="KRL67_003552"
     CDS             complement(2497..2892)
                     /locus_tag="KRL67_003552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH1524388.1"
                     /translation="MNILKMKKTGIFLGGVLFGTVGVKILSSDCVKKTCTRATAGALK
                     VKDSVLETTTKIQENVEDILAEAKEINEQEDLSTSKVNVVQDTSKYEQEVIKTETIKT
                     AEDTNSNSIKCEEIKDNVIEEKNSTCTTE"
     gene            3833..4021
                     /locus_tag="KRL67_003553"
     CDS             3833..4021
                     /locus_tag="KRL67_003553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH1524389.1"
                     /translation="MNKNNNLVIICMFIGMILGMSIGCAIGISKGNVGITMCYGLVFG
                     MIIGICIGTVIKNSNKKE"
     gene            complement(4203..4643)
                     /locus_tag="KRL67_003554"
     CDS             complement(4203..4643)
                     /locus_tag="KRL67_003554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3788 domain-containing protein"
                     /protein_id="HBH1524390.1"
                     /translation="MYERMLDKSNKPCLSQIIEYIGKDGYARLCKLEAYLETQYNLSR
                     ELRFPFGNNYGWGYKYSHKSFHLFYVFFEKGAFTVLIQIGDKQVPAVEKTLVALTPKA
                     KKLWVTRYPCGNNGGWIKYRILEDADLLDIIQYIRAKKSPPKQN"
BASE COUNT         1559 a          669 c          643 g         1915 t
ORIGIN      
        1 aaatctaaac ctatctgatt attagtattt tcaaatactt caatatctac atcagtacag
       61 cataatgata cataatatct accacttggt tctttagata tagtagcatt aagtattctc
      121 ccttgaggta cttgtttatc acttatctta accataccaa gtttaggtag ttttatatgt
      181 tgactaaagt acataatgtt tccatttgta aagttagttt tatatgaaaa tctattagtt
      241 ttctttgatt taaatttagg aaatcctgcc ttttttttaa agaattttca tatgcattat
      301 ctaaatcttt taaggcgttt tgtagtgaaa atttgtcagg ttccttaagc caatttaatt
      361 ctttttttaa attggtcaaa tcagaactac actgtttata agtaaaagtt tctttatcgt
      421 ttttataaac atctattctt ttagcaagat atttattata tacaaatcta caacatccaa
      481 aagttttttt aattagctct tgttgttttt tatttggata cattctaaac ttataagctt
      541 tttccactac tatcacctca ctttacttgt tttttctgat tattaatata ttttcttatc
      601 tgctcctctg tattttctga tacagtagca acgaaataac taggattcca aagatgtctc
      661 cccataatac tggatgtcta tatttgacat actatatgca tatcctctac catgagtaac
      721 ttctactttt catcacctat cataagatta aaatattcaa ttacttaaaa caagtatttt
      781 gaaggttttt tatatttatt tttaaataac atatataaga accgtaagtc ttaactttta
      841 tcatttttga ggttgtcgtt cacataagtt cgctactacc tatgcagttc tcttatgaac
      901 ttcttacact ttcatgtaag cacagactat atcttatcct tcggcattat ccgctaaggt
      961 ctacccactt ccactaccaa tagcttgtag tgtacttccc tcaagaggaa tagtcgttga
     1021 agtttctcct attcggagct tacctgctga ttgcccattt taaagtactt aggatttaac
     1081 cttataccat ctaaccaatt ttttctactt tcgtaacttt cacacttagg tttatttcat
     1141 ccttatgttt tagtttggtt agctttaggg gtttttagca attcaagtag tattggatag
     1201 gtgttatcct atctctacat ataagtttcc ctatatgctg actaatttat ctactaactt
     1261 atgactaaag tcacaagtat gcgtagctat tttttaatca aatacaatag tatctgcaca
     1321 tgcaacacct tcaaggaatt ttccaccttt tacagtaatg ttgtgattgt tacattctct
     1381 catggctgat agtactgaaa tagggataga gagttttaat gaacaagaga aatctaccat
     1441 taatatagag agtgatttta tagggtttct agtaattgca tatgttaata tacttactaa
     1501 aaagctgtat ggaactaatg tatctgctag atgttcaaat ttactctcta cagaagattt
     1561 tagtttttca gattcctcaa tcatagttac tattttctgg aatctagtag taccagcttt
     1621 ttcctttaca cacataacaa tattgccttg ctctattaca gttccagcat atatgtaaga
     1681 gctttctttt ttgttaactg caagggattc gcctgtaagt gatgcctgat tgaccattgc
     1741 ttctcctgat actataactc catccaatgg aatcatattt cccatagtta tactgactaa
     1801 atctccttcc tttatctctg aaattggaat tagtatttca gtgtcacctt ttcttaacca
     1861 tactttttca atgtttagtg acatactttg agccaagtcg tttatagatt tttttctagt
     1921 ccattcttct aataattcac cgataccaag aagaaacata actgaaccag ccgtattgat
     1981 atcttttcta aaaatagaaa tacctatagc agtggcatct aaaacagaaa tttctatttt
     2041 tttatttagt agggaagaga gacctttttt aaaatatttt atagaatgaa gtccaattaa
     2101 aaaaaatctc aaggactttg ggaaaaatat tttggatata cttttgacta gcagtttatt
     2161 tactaatttg tctctataat aattgtttaa ttccctactg gtatgttcag gaactagatt
     2221 ttctattttg ctattgttaa aactgaattt agtgatttct cttattatgt attctctttc
     2281 accagtgtaa taaatgactg catcaccagt tctatcgtaa acttttgctt tggtaatacc
     2341 atttatagaa gttaaataat ataagagagt atcggactgc ttgttgttta atttgccaag
     2401 ttgtaagtca aagcgtatac gacctcttat ttcgtgtttt atataaaaat tcaatttact
     2461 tcctcctgat attattaatc ataataatca ttaaatttat tctgttgtac aagtactatt
     2521 tttttcttca attacgttat ctttgatttc ttcacattta atgctattag aattagtatc
     2581 ttctgcagtt tttatagttt cagttttaat aacttcttgt tcgtatttag aagtatcttg
     2641 tacaacgttt accttagatg tagataagtc ttcttgttcg ttgatttctt ttgcttcagc
     2701 taaaatatct tctacatttt cttgaatttt tgtagttgtt tcaagaacag aatctttaac
     2761 tttcaatgct cctgctgttg cacgagtgca agttttttta acacagtcac ttgaaagtat
     2821 cttaacacct acagtaccaa aaagtactcc tcctaagaat atccctgttt ttttcatttt
     2881 taaaatattc attttacatt atcctttcat ttagttagat tttgtttgta cttatgtata
     2941 aggtaacaaa tgctaaaaat agagtatgtt ttacaaaatt agtttagaga gtttagtaat
     3001 tttatttgat ttatattagt aaatttatac gagttttaca tgtgtagttt atgtagttga
     3061 aataattctt atctagttta tatatgtaat ttatatatgt agcttatata gttgaggtaa
     3121 tactttatat agcttataga gttcatataa atttataacc gaatactaca cagtttatat
     3181 ataacatatt gttcgagctt atagagttca tataaattta caacttatat aaattttttg
     3241 tactttgtat tttagagagt ttttgatatt tagtccaaaa attttaaaat acagtaaaaa
     3301 taataattgt tattaataga tttgtaaaat catatgacat ttattaccat ttatgcaatt
     3361 aagtatagca tgtgcgaatg gtattttcaa cagaaaatga taatttatat caattaattt
     3421 acttgatttg taatagagca tcaaaaagta tacttattta tatatgggaa tgaaaggcaa
     3481 agttttagtt ttttcaatta aagtgcgtta gatatattat tataaatgga cattacttta
     3541 agtataataa tgattaggag ggagtgttta tgatgtataa atgaataaca atatagttta
     3601 tgattgtaaa tattatgtaa atataaaagt tatttaaata gttttggaaa attgatatta
     3661 ttgtgtctgg gagattatat ttgcttcaaa aagaggaagt tatgacaaga aatgtgttgt
     3721 ttgacattta tacaagcaga tattagaata aaataaagtt aatttatatt tctttgtttt
     3781 aagctaagaa ggcgagctag gcaatttaaa atatatggaa tggagaaaaa ctatgaataa
     3841 aaacaacaat ttagttatta tatgtatgtt tataggcatg atacttggaa tgtctatagg
     3901 ttgtgctata ggtatttcta aaggaaatgt aggtattaca atgtgttatg gtctagtttt
     3961 tggaatgata attggcatat gtatagggac tgttatcaaa aactctaata aaaaagaata
     4021 aatgaaattt gatttactag gatgtctaat tatgatttat ttgtgataaa actttttaat
     4081 tatctgtaaa aaatcgaata agacatacta aataagatac taatagctat tttttatatt
     4141 atttttttaa tatggaaaga atggaattta tatacactat atttaaatca caaaaataaa
     4201 agttaatttt gtttaggtgg agattttttt gctcttatat attgaataat gtcaagtaag
     4261 tctgcatctt ctaaaatacg atacttaatc caacctccat tattgccaca aggataacga
     4321 gtaacccaga gttttttagc ttttggagtc aaagctacca atgttttttc tactgctgga
     4381 acttgcttgt cacctatctg aatcagaaca gtaaatgcac ctttttcaaa aaaaacataa
     4441 aataaatgaa aagatttatg gctatatttg taaccccaac cataattatt tccaaaagga
     4501 aatcgaagtt ctcttgataa attatattga gtttccaagt aagcttccaa cttacacaat
     4561 cgtgcatatc catctttacc tatatattct attatttgag acaagcaggg tttattgctt
     4621 ttatcaagca tacgttcata cattagaatt tacctccgtt tttactttta attatagtaa
     4681 agtaattatg acaacagtat gtcaagtttt tatatattat tcaagttttt atgtattatt
     4741 atactaatat gtaaaagtat aaagtaatta gttttagcta atagaa
//
LOCUS       DAEPXF010000237         4782 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain HB27
            SAMN13376389-rid12479423.denovo.245, whole genome shotgun sequence.
ACCESSION   DAEPXF010000237 DAEPXF010000000
VERSION     DAEPXF010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13376389
            Sequence Read Archive: SRR10518432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4782)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4782)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001069624.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001069624.1
            Genome Coverage       :: 315x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/15/2021 01:54:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,165
            CDSs (total)                      :: 4,076
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 89
            rRNAs                             :: 10, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 10 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 19 of 69
            Pseudo Genes (incomplete)         :: 44 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 10 of 69
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4782
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13376389-rid12479423.denovo.245"
                     /strain="HB27"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="China: Hebei"
                     /collection_date="2017"
     gene            complement(<1..758)
                     /locus_tag="KRL67_003555"
     CDS             complement(<1..758)
                     /locus_tag="KRL67_003555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HBH1524391.1"
                     /translation="MFAEERIQAILNILKKEGRVTVKELSSKFNVTEDCIRKDLKNIE
                     KISSIRRIYGGAVLARETLENQDTKDRKEINIPTKKIIAEKAFNLIEDRETIFLDIST
                     INILLAKLLAESNKKVTLVTNMLDILNVVTSKPNNLNIISTGGLLNLSLDGFVGTPTI
                     EFISKYKFDKTFMGSCGVDVFNSSLTTFEIEDGLTKKAIINSSKKVFIVMEDKKFKFD
                     GNFKFAHLEDISSIITEKAPTPDIVDILSEFNVNV"
     gene            1032..1706
                     /gene="fsa"
                     /locus_tag="KRL67_003556"
     CDS             1032..1706
                     /gene="fsa"
                     /locus_tag="KRL67_003556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HBH1524392.1"
                     /translation="MQLILDTGNVEEIKELCTCLPIDGVTTNPSIVSKEKKNFKQLIN
                     EIGEIIGEDMPIHAQVLSTKYEEILEEALYISSLRKNIYVKIPVTQDGLRAIKDLHRK
                     GVKITATAIFTAHQGFLAAKAGANYIAPYVNRLDNISGDGVAMVSELIKIIDTYKMDT
                     KVLAASFKNAQQVIELMQHGVHSVTVPYDICKSMMNHPLTDWSVDKFIEDWENTFGKG
                     SKTNNI"
     gene            complement(1828..3222)
                     /locus_tag="KRL67_003557"
     CDS             complement(1828..3222)
                     /locus_tag="KRL67_003557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HBH1524393.1"
                     /translation="MGIILKPKQFYMTVIATIILYFSLNYSFNIKFSICLSFLTILIW
                     AVDSVEKTLVALFFVILAFIFNIAPIKVILQFLFTENFYIIILAYIITNAVTKTGVAK
                     IISEKLILNTVNTPKKMILLSYVLGILLIFFIPQPFPRVILVSAFYKEFLKEQQITED
                     SKSILLFSIFTASTFTSMFFVNGDTLLNYVVLELGNCNINWGQWAFYMSVPTIITCFI
                     TYFLFIFVFKKELHSIKFISNENLEVNKLHFSYSNLKKEFNLLNKEQKHVFLCLGIMF
                     FMFLTQFIHNINTLIIMSICVLLLLLRKIVGFSTLKEINWKVLIFFIAAFSIGGVLKY
                     SGVVDIMGNYLVKFIPNSSEIISILFLITLTIILNICLGSAVTTSSVVIPLLGSLHIL
                     KENSVTLCLFVYIIVSIQYILPFHHATIMVGHGENLYNSKVIFKYGLTLIPLTYFIII
                     CITFPWWKFIGLEI"
     gene            complement(3300..4646)
                     /locus_tag="KRL67_003558"
     CDS             complement(3300..4646)
                     /locus_tag="KRL67_003558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HBH1524394.1"
                     /translation="MKDLTTGHEGKSIFFFAMPMLIGSLFQQLYNTADSIIVGRFIGK
                     EAMAAVSGANPIMFLLVAALMGVSLGFSILVSQFYGSGDLKKVKATIDTTYILLFIGS
                     ILISILGIVFGGPMLKLMNTPESVFAQSKLYLTIIFSGILFSAGYNSVSAILRGLGDS
                     VTPLYFLIIATILNIVLDLTFIVVLRMGVEGVALATIMAQAVSFIISIIYLNKKHEVL
                     KFKIKGIVYDNKIFKDGLRLGLPSGVQQMLFSIGNMALQFLVNSYGTSAMAAFGAGLR
                     IENFISLPIMNLGSAVSTFVAQNIGAGENERVKKGIRESIKMTLVLAVTVIALILLFR
                     ENLIALFNTDKDVIKIGSSYLFIIGPFFLFIGTSFVLSSAMKGAGDSMFALISSIVSL
                     WLGRLPASYMLSKFFGTDGIWMGIPFGWTLGLIVTVIYYKKGYWKTKAIVNHRINE"
BASE COUNT         1852 a          677 c          626 g         1627 t
ORIGIN      
        1 acatttacat taaattctga taaaatatct actatatcag gagtaggcgc tttttctgta
       61 attatagaac taatatcttc taaatgagca aacttgaaat ttccatcaaa tttaaacttt
      121 ttatcttcca taactataaa tactttttta cttgaattta ttatagcctt ctttgtgaga
      181 ccatcttcaa tttcaaaagt tgtaagtgaa gagttgaata catctactcc acaacttccc
      241 ataaaagtct tatcaaactt atatttcgat ataaactcta tagttggagt accaacaaaa
      301 ccgtctaaac tgagatttaa taaacctcca gtggaaatta tatttaaatt atttggtttt
      361 gaagtaacta catttagtat atccaacata tttgtaacaa gtgttacttt tttattactt
      421 tcagctaaca actttgcaag caagatgtta attgtagaga tatctaaaaa tatagtttct
      481 ctatcttcaa ttaaattaaa tgctttttct gctattattt tctttgttgg aatattaatc
      541 tcttttctgt cttttgtatc ctgattctct agtgtttctc tagctagtac agctcctcca
      601 taaattcttc ttattgaaga aatcttttct atatttttta aatcttttct tatacaatct
      661 tctgtaacat taaatttaga actgagctct ttgacagtaa ctctaccttc tttttttagg
      721 atatttaata tagcttggat tctttcttct gcaaacattt tctacctctt ttcgcaatat
      781 agtattatag taaatattat aagataaaat acaatatatc aactatttta acatgaaata
      841 caaaattaat ttaatataaa aagtatgttt tttctaacta tatatttaga atttagtaaa
      901 taaaaaaatt ataaaactaa gaaggaaaag taaaacaata ataaaaaata aaaaacaata
      961 agcaaaatgt tgaaaataat accaaatcaa tatataataa gaataaacaa agattaaagg
     1021 gggatatttt aatgcaacta atcttagata caggtaatgt ggaggagatt aaggagttat
     1081 gtacttgctt accaatagat ggtgtaacta caaatccaag tattgtttca aaagaaaaaa
     1141 agaattttaa gcaacttata aatgaaattg gtgagattat aggtgaggat atgcctatac
     1201 atgctcaagt gttaagtaca aagtatgaag aaatattaga agaagcatta tatataagta
     1261 gtttgagaaa aaatatatat gtaaaaatac ctgtaacaca agatggttta agagctataa
     1321 aagaccttca cagaaaaggt gtaaaaataa ctgctactgc aatattcact gctcatcaag
     1381 gttttttagc tgcaaaggct ggagcaaatt atattgcacc ttatgtaaat aggcttgata
     1441 atatatctgg ggatggagta gctatggttt cagaacttat aaaaataata gatacttata
     1501 aaatggatac aaaagtactt gcagccagtt ttaaaaatgc acaacaagtt atagaattaa
     1561 tgcaacatgg ggtgcatagt gttacagtac catatgatat ttgtaaatct atgatgaatc
     1621 atccattaac tgattggagt gtagataagt ttatagagga ttgggagaat acttttggaa
     1681 aaggaagtaa aacaaataat atttagtatt atggtaaaaa agtagttttg tccaatttca
     1741 caagaactta tataaaaaga tgaaaatatg aattttcttt ttaataattt cgttattatc
     1801 ttaagtcaat tcatattttc atctttttta gatttctagt cctataaatt tccaccaagg
     1861 gaatgttata catattatta taaagtaggt taaaggaatc aaagttaaac cgtatttgaa
     1921 aataaccttg ctattataca gattttcacc atgaccaacc attattgtag catgatgaaa
     1981 tggcaatatg tactgtatac ttactataat gtagacaaat agacataaag taacactatt
     2041 ttcctttagt atatgtaaac ttccaagtaa cggtattact acagatgatg tagtgacagc
     2101 acttccaaga catatattca agattattgt gagagttatc aaaaacaata tcgaaataat
     2161 ttctgaagag tttggtataa actttaccaa ataatttccc attatatcta caactccaga
     2221 gtactttaac actcctccaa ttgaaaatgc tgctataaaa aaaataagga ccttccaatt
     2281 tatttctttt aaagtactaa atccaactat ttttcttagt aataaaagaa gtacacaaat
     2341 tgacattata attaatgtgt ttatgttatg tatgaactga gttaaaaaca taaaaaacat
     2401 tattcctagg cataaaaata cgtgtttttg ctctttgttt agcaaattaa attctttttt
     2461 taaattagaa tatgaaaaat gaagtttatt aacctcaaga ttttcattag aaataaattt
     2521 tattgaatgc aattcttttt taaagacaaa gataaataaa aaataagtta taaaacaagt
     2581 aattatagtt ggaacagaca tataaaaagc ccattgaccc caatttatat tacaatttcc
     2641 aagttctagt acaacatagt ttagaagggt gtctccattt acaaaaaaca ttgatgtaaa
     2701 ggtagatgct gtaaatatac taaataaaag tatagatttt gaatcttcag ttatctgttg
     2761 ttcttttaaa aactccttgt aaaaagcaga tacaagaata acccttggaa atggttgtgg
     2821 gataaaaaat attaaaagta tacctaaaac atacgataaa agaatcattt ttttaggggt
     2881 atttacagta tttaaaatta acttctcaga aataattttt gcgacacctg tttttgtaac
     2941 tgcattagta attatgtatg caagaataat tatgtaaaaa ttttctgtaa ataaaaactg
     3001 taaaataacc tttattggtg caatgttaaa gataaacgct agaattacaa aaaacaatgc
     3061 aacaagagtt ttttcaactg agtctacagc ccatatcaag atagttaaaa aagataagca
     3121 tatagaaaat tttatgttga aactgtaatt aagagagaag tagagaatta tggtagctat
     3181 aacagtcata taaaattgtt ttggcttaag aataataccc atttttatat tcacctccat
     3241 aatttattat atcataatgg aggtgaatac aatgaatgtg aatacatttt ataaaaagat
     3301 tattcattga ttctgtgatt tacaatagct ttagttttcc aatacccttt cttatagtag
     3361 ataactgtaa caataagacc taaagtccaa ccaaaaggaa tacccatcca aattccgtct
     3421 gttccaaaaa atttggaaag catataagaa gctggaagtc ttccaagcca taaagatact
     3481 attgagctta taagtgcaaa catggaatca ccagcacctt tcattgcact tgaaagtaca
     3541 aaagaagttc caataaataa aaagaatggt cctattataa ataagtaaga agagccaatt
     3601 ttaatgacat ctttatcagt attgaataga gcaattagat tttccctaaa tagcaaaatt
     3661 aaagcaataa ctgtaactgc taaaactaag gtcattttta tagattctcg aatgcctttt
     3721 ttaactcttt cattttctcc agcacctatg ttttgagcaa caaaagtaga aacagcagat
     3781 ccaagattca taataggtaa gcttataaaa ttttctattc taagaccagc accaaaagct
     3841 gccatagcag atgtaccata actattaact aaaaattgaa gagccatatt tccaatagaa
     3901 aacaacattt gttgaactcc acttggaagt cctaatctaa gcccatcttt aaatattttg
     3961 ttatcatata caattccttt gattttaaat ttaagcactt catgtttttt atttaaataa
     4021 attatactta ttataaatga tactgcctga gccattatag tggcaagtgc aactccctca
     4081 acacccattc ttagcactac aataaaagtt aagtctaaaa ctatatttag aattgtggct
     4141 attatcagaa aataaagtgg agttactgaa tctccaagtc ctctaagtat tgcacttact
     4201 gagttatatc cagcagaaaa taatattcca ctaaatatta tagtaagata caattttgat
     4261 tgagcaaaaa cggattctgg tgtattcata agcttaagca tggggccacc aaatactatt
     4321 ccaagaatac ttattaatat agaaccaata aataaaagta tatatgtagt atcaatagta
     4381 gcttttacct tttttaaatc tcctgagcca taaaattgtg acactaaaat agaaaatcca
     4441 aggctaacac ccataagtgc tgccaccaat aaaaacatta tagggttagc tccagagact
     4501 gccgccattg cttcttttcc gataaatcta ccaactatta tactgtcagc tgtattgtat
     4561 agttgttgaa ataaactacc aataagcata ggcatggcaa aaaagaatat ggattttccc
     4621 tcatgacctg tagttaaatc tttcacctta ttcctccaaa taaaaaatta ttaatttatt
     4681 atagtataat cttaacataa actttatatc caaactataa ataaattgta tcaaaatatc
     4741 atatattttt ttaaaatgta aagaatataa ttataatttg aa
//
LOCUS       DAEPXF010000238         4764 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain HB27
            SAMN13376389-rid12479423.denovo.246, whole genome shotgun sequence.
ACCESSION   DAEPXF010000238 DAEPXF010000000
VERSION     DAEPXF010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13376389
            Sequence Read Archive: SRR10518432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4764)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4764)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001069624.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001069624.1
            Genome Coverage       :: 315x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/15/2021 01:54:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,165
            CDSs (total)                      :: 4,076
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 89
            rRNAs                             :: 10, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 10 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 19 of 69
            Pseudo Genes (incomplete)         :: 44 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 10 of 69
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4764
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13376389-rid12479423.denovo.246"
                     /strain="HB27"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="China: Hebei"
                     /collection_date="2017"
     gene            complement(82..1368)
                     /locus_tag="KRL67_003559"
     CDS             complement(82..1368)
                     /locus_tag="KRL67_003559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HBH1524395.1"
                     /translation="MIVGIIGPSDSGFKIKDDLKQIDSDLKTKIYVREKVVDTIEVIS
                     KCEDECDAIIFTGCAVYEFIKSKYEISKPHSFVPRSGTSIMKAFWAIKSANIKLDKFS
                     IDVVDRYVVEDALKEFDIKATSVFCNPFSLEVGESELVEWHIKLFEENKTDIMLTGFG
                     AVYNELKERGYPVFRLQATIQLIKESYDKVKSEYALSKARFSQIAVEILSLIDYKEKI
                     DNYYSDMIKKSDMDKLVVNYVRSIQGSLFLLGRNDYVVFVHKGVADNEYNYDKLFNLK
                     REIKDMGFSLSIGIGTGVTAYQAENNAHKALRHSIDSKEIGIYLVDEDENIRGPLASE
                     NELNYSLMLSDEKVIEISKKTGMSCESVAKIIAISENRKSKIYDSKELAEYLNVSERS
                     ARRILNKIVNAGLGRICAKETSIGGGRPKNITEILF"
     gene            complement(1491..4589)
                     /locus_tag="KRL67_003560"
     CDS             complement(1491..4589)
                     /locus_tag="KRL67_003560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional diguanylate
                     cyclase/phosphodiesterase"
                     /protein_id="HBH1524396.1"
                     /translation="MDVINLIDELDEIVYVSSLDTYELLYINEIGKKAMGLNDISHVK
                     CYKALQGKDSPCEFCTNSILKEDKYYIWENTNTRTNRHFILKDKLIKWKGKPSRLEIA
                     LDITEKEDISKSIAEKLEIKELLVGCIKHLITESNFSDAVNLVLTNIGKFHKADRAYV
                     FEVSEDRKKVTNTYEWCNEGVDEQKQSLTNLDIKELKNWFLLFEQKGFVIIDDIENIK
                     EEQPIEYEILKPQKIESLMASSLKQDDEIIGFIGVDNPHKAVGDTSLLTSLSYFLLTE
                     MKKRKVENKLNYMSYYDFLTGAYNRNKYIKYIDNFQKGKVKSIGVVFVDINGLKDIND
                     NFGHANGDSAIVSACVIIKKYFHSYNVYRIGGDEFVVLCEDISKQLFEELVSWLSEEF
                     KDEMIYSVAVGSVWSDDNIDIHKLIKVADERMYQDKKRYYNKNKLRNNEMEVISDLAD
                     LTNNILFKHKELADKVYFDDLMIKGYSKAYEKLAYNTYDIILSICIDTNLITPIYQSE
                     SNIFSFPIKENLEKTFSYIKKYLIHPDDIILFDDNHLLKSLDSLNNSKQDDLELNFEY
                     RRLGIDKKYYWALIYMLKISSRNKLGEKQTHILVAIKNIDYLMQLHKNQKDTLTGLYN
                     YESFCEQINLMLKRYPDKRYAIIMMDIDKFKVINDIYGVRGGDKALIYVAELIQKNIG
                     SNGICCRMYADVFCIFYESYSDRDIHKVIYKIKNSLNRKKFEYMLVPCFGVYKILDNH
                     IPITNACEMAGYAHKKVKKQSINNYIFYDDTIRNDALEEKQMEREMEYALENGQFHVY
                     LQPKYNLNANKIVGAEALVRWVHPEKGIIPPIKFISLFEKNGFIIRLDMYVWEQVCSL
                     IRKWIDLGEEPVPISVNVSRLHLHNPHFKEIVLSLINKYGIPKSFLELELTESLFIKN
                     IKLFTKIIADLRMDGIVLNMDDFGSAYSSLNMLKNINIDTLKIDCGFFNEEGITDREK
                     IVLKHTIAMAKDLDMDVVAEGVETKEQAEFLVSLDCVVIQGYYYCKPIPSADFEKLLY
                     QK"
BASE COUNT         1550 a          845 c          444 g         1925 t
ORIGIN      
        1 taataagctt taatacttta tatagtaatt tgttgtagaa ttttatataa tataatgatt
       61 tatacaattt tataaaatat attaaaacag tatttcagta atattttttg gtcttcctcc
      121 accaatacta gtctcttttg cacatattcg acctaaacct gcatttacta ttttatttaa
      181 tatacgtctt gcacttcttt cacttacatt tagatactct gctaactctt ttgaatcata
      241 aatcttactt tttctattct cacttatagc aataattttt gcaactgact cacaactcat
      301 tccagtcttt ttagatattt ctattacctt ttcatcagat agcatcaaag aataatttaa
      361 ttcattttcg gaagctaaag gtcctcttat gttttcatct tcatctacta aatatatacc
      421 aatttctttt gaatctatgg agtgtcttaa tgctttatgt gcattatttt cagcctgata
      481 cgcagttact ccagtaccaa ttcctatact aagagaaaag cccatgtctt ttatctctct
      541 ctttagattg aataacttat cataattata ctcattatca gctacacctt tatgaacaaa
      601 tacaacataa tcatttcttc caagtaaaaa taacgaacct tgaatgcttc ttacatagtt
      661 tactacaagc ttatccatat ctgatttttt tatcatgtca gaataatagt tatctatctt
      721 ctctttataa tctattaggc tcaatatttc gacagcaatt tgagaaaatc ttgccttgct
      781 tagagcgtat tcactcttta ccttatcata actttctttg attaattgta tagtagcttg
      841 taaacgaaat acaggatagc ctctttcttt taattcatta tatactgcac caaaaccagt
      901 taacattata tccgtcttat tttcttcaaa taattttata tgccattcta caagttcaga
      961 ctctcctact tccaaagaaa atggattaca aaatacactc gttgctttaa tatcaaattc
     1021 tttaagtgca tcttccacaa catatctatc aactacatct atactgaatt tatctaattt
     1081 tatatttgca ctttttattg cccaaaatgc cttcattata cttgtcccac ttctcggtac
     1141 aaatgaatgt ggcttgctta tctcatactt agattttata aattcataaa ctgcacatcc
     1201 tgtaaatata atagcatcac attcatcttc acatttagaa ataacttcta ttgtatctac
     1261 tactttttct cttacatata ttttagtctt taaatcactg tctatctgtt ttaaatcatc
     1321 ctttatctta aacccagagt ctgaaggacc tataataccc actatcataa acttcgctcc
     1381 taatccataa atattatact ttttaaaaat tatatcctca ctattttcta cttctattta
     1441 attattttga actactccat cctacctaca tttagaatgg aataattagc ttatttttga
     1501 taaagtaact tctcaaaatc agcacttgga ataggcttac aataataata accttgaatt
     1561 accacacaat ctaaactcac taaaaattca gcttgctctt ttgtctctac accttctgcc
     1621 actacatcca tatctaaatc ttttgccatg gctatagtgt gctttaaaac tattttttct
     1681 ctgtctgtga ttccttcttc attaaaaaaa ccacaatcta tctttaatgt atcaatatta
     1741 atatttttta acatatttaa tgaagaatat gctgaaccaa agtcatccat atttaaaaca
     1801 ataccatcca ttctaaggtc ggctattatc ttagtaaata attttatatt tttaataaat
     1861 aaactttctg taagctctaa ttctaaaaaa gattttggta taccatattt atttattaaa
     1921 cttaaaacaa tttctttaaa atgtggatta tgcaaatgta atcttgatac attaacagag
     1981 ataggaactg gctcttcacc taaatctatc cattttctaa tcaaagaaca tacttgttcc
     2041 catacataca tgtctaatct gataataaac ccattctttt caaatagtga aataaattta
     2101 attggaggta ttataccttt ttctggatga acccatctaa ccaatgcttc tgcaccaaca
     2161 attttatttg cattaagatt atacttaggt tgtaaatata catggaattg cccattttca
     2221 agtgcgtatt ccatttctct ttccatctgt ttttcttcaa gtgcatcatt tcttatagta
     2281 tcatcataaa atatataatt attgatactt tgtttcttta cttttttgtg agcatatcct
     2341 gccatttcac acgcatttgt aattggaata tgattatcca aaattttata aaccccaaaa
     2401 catggtacta acatatattc aaattttttt ctatttaagc tatttttaat cttataaatt
     2461 actttatgaa tatctctatc agaataactc tcataaaaaa tgcaaaatac atctgcatac
     2521 atacgacaac atataccatt agaaccaata tttttttgaa tcaactctgc aacatatatt
     2581 aaagctttat ctcctcctct aactccataa atatcattaa ttaccttgaa cttatctata
     2641 tccatcatta taattgcata gcgcttgtca ggatatcttt taagcattaa atttatttgt
     2701 tcacaaaaac tttcataatt gtatagccct gttaatgtat ccttttggtt tttatgtagt
     2761 tgcataagat aatctatatt ttttatagca actaaaatat gtgtttgttt ctcacctaac
     2821 ttatttctac tactaatctt caacatataa attaatgccc aatagtactt tttatctatt
     2881 cctaaacgtc tatactcaaa atttagttct aaatcgtctt gcttgctatt attaagacta
     2941 tcaagagatt ttagaaggtg gttatcatca aataatatta tatcgtcagg atgaattaaa
     3001 tattttttta tgtatgaaaa agttttttca agattttcct ttataggaaa actaaaaata
     3061 ttgctctctg attgataaat tggagtaatt aaattcgtat caatgcaaat agataaaatt
     3121 atatcatatg tattataagc taatttttca taagccttag aatatccttt tatcattaaa
     3181 tcatcaaaat aaaccttatc agctaactct ttatgtttaa ataaaatatt attcgtcaaa
     3241 tcagctaagt cactaattac ttccatttca ttatttctaa gtttgttctt attatagtac
     3301 ctcttcttat cttggtacat tctctcgtca gcaactttaa ttagcttatg tatatcaata
     3361 ttatcgtcac tccaaacact gcccactgct acactatata tcatctcatc cttaaattct
     3421 tcacttagcc aactcactaa ttcttcaaat agttgttttg atatatcttc acataataca
     3481 acaaactcat ctccaccaat acggtaaaca ttgtaagaat gaaagtattt ttttattatt
     3541 acacatgctg acacaatagc agagtcgcca ttagcatgtc caaagttatc attaatatct
     3601 tttaatccat ttatgtctac aaatacaaca cctatagatt taactttgcc tttttgaaaa
     3661 ttatctatat acttaatata tttgtttcta ttatacgcac cagttaggaa gtcatagtag
     3721 ctcatataat ttaatttatt ttctactttt ctttttttca tctcagttaa taaaaaataa
     3781 gatagggatg tcaaaagaga tgtatctcca acagccttat gtggattatc aactccaata
     3841 aaacctatta tttcatcatc ttgttttagt gaagatgcca ttaaactttc aattttttga
     3901 ggttttaaaa tttcatattc tattggctgt tcttctttta tattttctat atcatcaatt
     3961 attacaaagc ctttttgttc gaataataaa aaccaattct ttaattcctt aatatcaaga
     4021 tttgttagtg actgtttttg ttcatcaaca ccttcattac accattcata tgtattagtt
     4081 actttttttc tatcttctga tacttcaaaa acatatgctc tatcagcttt atgaaattta
     4141 ccaatattgg ttaatacaag atttacagca tcagaaaaat tagattctgt tataagatgt
     4201 ttaatacatc caactaataa ttcctttatc tctaattttt ctgctattga cttacttata
     4261 tcttcctttt ctgtaatatc aagagctatt tctaatcgtg aaggtttacc cttccattta
     4321 attaacttgt cctttaatat aaagtgtcta tttgttcgag tattcgtatt ttcccatatg
     4381 taatatttat cttcttttaa aatagagttt gtacaaaatt cgcagggaga atctttcccc
     4441 tgtaatgctt tataacattt tacatgggat atatcattaa gtcccattgc ctttttccca
     4501 atttcattaa tatataaaag ttcatatgta tctaaagaag aaacatatac aatttcatcc
     4561 aactcatcta ttaaatttat tacatccatt tatatatatc tccttttatt cattttccag
     4621 attgaatttt taaagttttt aaaattttgc tatatatttg gcaatgatat tatgtttctt
     4681 tatgttttaa aatagcacct taatgaggtt ctatttttta gtaaatagaa ttattattgt
     4741 gtaaatcgta taatatagtt ttgt
//
LOCUS       DAEPXF010000239         4734 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain HB27
            SAMN13376389-rid12479423.denovo.247, whole genome shotgun sequence.
ACCESSION   DAEPXF010000239 DAEPXF010000000
VERSION     DAEPXF010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13376389
            Sequence Read Archive: SRR10518432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4734)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4734)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001069624.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001069624.1
            Genome Coverage       :: 315x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/15/2021 01:54:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,165
            CDSs (total)                      :: 4,076
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 89
            rRNAs                             :: 10, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 10 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 19 of 69
            Pseudo Genes (incomplete)         :: 44 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 10 of 69
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4734
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13376389-rid12479423.denovo.247"
                     /strain="HB27"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="China: Hebei"
                     /collection_date="2017"
     gene            156..1064
                     /locus_tag="KRL67_003561"
     CDS             156..1064
                     /locus_tag="KRL67_003561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl-radical enzyme activating protein"
                     /protein_id="HBH1524397.1"
                     /translation="MNPLVINLQKCSIHDGPGIRSTVFFKGCPLECVWCHNPESQTYT
                     KQVLYNEERCSKCEACINICPHKAIYKGETKICLDQDKCEFCETCLDYCVNNAREIVG
                     QEYSVRDLVKEIEKDRIFYEESGGGVTLSGGEVMAQDMDFICGVINMCKSKGIHVAID
                     TCGYAKSENYERVAKCADLFLYDIKLIDEDKHIKFTGKSNDLILKNVKILSELGANIN
                     IRIPLIVGVNVDDENLEVKKMIEFLKPLNIQAVSLLPYHNIGKHKYDKIYKKYEGEEL
                     QRPSEEKLEEIKRLFEASNFNTKIGG"
     gene            1069..3438
                     /locus_tag="KRL67_003562"
     CDS             1069..3438
                     /locus_tag="KRL67_003562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl radical protein"
                     /protein_id="HBH1524398.1"
                     /translation="MARGTFERTKKLREESINAEPHISIERAVLMTEAYKKYEGSVEI
                     PVLRALSFKHYIENRTLSINDGELIVGEKGDSPNGAPTYPEICCHTMEDLEVMHNRDI
                     INFSVSEEARKIHKEEIIPFWKKRQTRDKIINAMTPEWLAAYEAGMFTEFMEQRAPGH
                     TVCGDTIYKKGFLDLKKDIEARLRELDFLNDLDAYNKKADLEAMAIACDAMVILGKRY
                     AEKARQMAEEETDEAKKKDLLLIAETCDVVPAHKPETYHQAIQMYWFVHIGVTTELNI
                     WDAFTPGRLDQHLNPFYERDVENGILDRDRAQELLECLWVKFNNQPAPPKVGITLKES
                     STYTDFANINTGGINPDGQDGVNEVSYIILDVMDEMKLIQPSSNVQISKKTPQKFLKR
                     ACEISRKGWGQPAFYNTEAIVQELMEAGKTIEDARLGGTSGCVETGCFGKEAYVLTGY
                     MNIPKILELTLNNGYDPISKKQIGIETGDPRNFKSYEELFEAFKKQLHYMIDIKIEGN
                     AVIENICAKHMPCPLMSTIVDDCIEKGKDYQRGGARYNTRYIQGVGIGTITDSLTAIK
                     YNVFDKKKFDMDTLLKALDVNFEGYEAILNLVSNKTPKYGNDDDYADEIMQEIFNAYY
                     NEVTGRPTVCGGEYRVDMLPTTCHIYFGEIMGASPNGRLCAKPVSEGISPEKGGDTNG
                     PTAVIKSCAKMDHIKTGGTLLNQRFAPSVVQGEKGLDNMANLVRAYFNMDGHHIQFNV
                     FDKNVLLEAQKNPQDYKDLIVRVAGYSDHFNNLSRTLQDEIIGRTEQTF"
     gene            3763..4566
                     /gene="proC"
                     /locus_tag="KRL67_003563"
     CDS             3763..4566
                     /gene="proC"
                     /locus_tag="KRL67_003563"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HBH1524399.1"
                     /translation="MKTLGFIGSGNMGSAMIGGIVQSGLVKAEDITVSDLSQVALDKV
                     KEEFGVNTTTDSNVAVTNSDVVIVALKPNICEKALTPLKGLIDVNKIIVSIAAGKTIE
                     SLESFIGSDKKIVRVMPNTPALVGEGMSALCKNSNVTDEELNMIKALFESFGEAEIVS
                     EYLMDTVTGISGSSPAYVFMFIEAMADAAVLAGMPRQQAYKFASQAVMGSAKMVLETG
                     KHPGELKDMVCSPAGTTIEAVKVLEEEGFRAAVIKSIVACIDKSKDMSK"
BASE COUNT         1907 a          510 c          914 g         1403 t
ORIGIN      
        1 ataaaatttt ttaatattac taaaaatata tagacaaaat aataacaaat atagtattat
       61 gtaattgtag aaatgctttt cataggtata ttctatgaag ataaattaca taaaaatgaa
      121 aaaatggaat ggatattatt taggggtgaa aaatcatgaa tccattagtt ataaacttac
      181 aaaaatgtag catacatgat ggaccaggga taagaagtac agtatttttt aaagggtgtc
      241 cacttgaatg tgtttggtgt cataatccag agagtcaaac atatacaaaa caagttttgt
      301 ataatgaaga aagatgttca aaatgtgaag catgtataaa tatatgccct cataaggcaa
      361 tttacaaagg tgaaacaaaa atatgcctag accaagataa gtgtgagttt tgtgaaactt
      421 gcttagacta ttgtgtaaac aatgcaagag agattgttgg tcaagagtat tctgtaagag
      481 acttggttaa agagatagag aaggatagaa ttttctatga agaatcaggt ggtggagtaa
      541 cactatcagg tggagaagta atggctcaag atatggactt catttgtgga gtaataaaca
      601 tgtgcaaatc aaagggaatc catgtagcaa ttgatacatg tggctatgca aagtcagaaa
      661 attatgaaag agttgctaaa tgtgcagatt tgtttttata tgatattaag cttattgatg
      721 aagataagca cataaaattt acaggaaaat caaatgactt aatactgaaa aatgttaaga
      781 ttttaagtga attaggggct aacataaata taagaatacc attaattgta ggagtaaatg
      841 tagatgatga aaatttagag gttaaaaaaa tgatagagtt tttaaagcct ttgaatatac
      901 aagcagtaag tcttcttcca tatcataata ttggtaaaca caagtatgat aaaatttata
      961 aaaagtatga aggagaagaa ctacaaagac catcagaaga aaaattagag gaaataaaaa
     1021 gactgtttga agcaagtaat ttcaatacta agattggagg gtaataaaat ggcaagagga
     1081 acttttgaga gaactaaaaa attaagagaa gaaagtatta atgcagaacc acacatatct
     1141 attgaaagag cagtacttat gacagaagct tataaaaaat atgagggaag tgtagagatt
     1201 cctgtacttc gtgctttatc atttaagcat tatattgaga atagaactct tagcataaat
     1261 gatggagaat taatagtagg tgaaaaagga gattctccta atggagcacc aacatatcca
     1321 gaaatttgtt gtcatacaat ggaagattta gaagtaatgc acaatagaga tataataaat
     1381 tttagcgtat ctgaagaagc tagaaaaata cataaagaag aaataattcc tttttggaaa
     1441 aagagacaaa caagagataa gataataaat gcaatgacac ctgagtggtt agcagcatat
     1501 gaagcgggta tgtttacaga gtttatggaa caaagagctc caggacatac agtttgtgga
     1561 gatacaatat acaaaaaagg atttttagat ttaaagaaag acatagaagc aagattaaga
     1621 gagttagatt ttcttaatga tttagatgca tataataaaa aagctgactt agaagctatg
     1681 gcaatagctt gtgatgcaat ggttatatta ggtaaaagat atgctgagaa agcaagacaa
     1741 atggcagaag aagaaacaga tgaagctaag aaaaaagatt tattactaat tgctgaaact
     1801 tgtgatgttg taccagctca caaaccagaa acttatcatc aagctataca aatgtattgg
     1861 tttgttcata taggggttac tactgaactt aatatatggg atgcttttac tccaggaaga
     1921 cttgatcaac atctaaatcc tttctatgaa agagatgtag aaaatggaat actagataga
     1981 gatagagcac aagaattact tgaatgttta tgggttaaat ttaacaatca gccagcacca
     2041 ccaaaagttg gtataacatt aaaagaaagt agcacatata cagattttgc aaatataaac
     2101 actggtggaa taaatccaga tggtcaagat ggtgttaatg aagttagtta tataatactt
     2161 gatgttatgg atgaaatgaa attgatacaa cctagttcaa atgttcaaat aagtaaaaag
     2221 actcctcaaa aattcttaaa gagagcttgt gaaatatcaa gaaaaggatg gggtcaacca
     2281 gcattttata atactgaagc aatagttcaa gagcttatgg aagctggaaa aactatagaa
     2341 gatgcaagac ttggtggaac cagtggttgt gttgaaactg gatgttttgg taaagaagca
     2401 tatgttctaa ctggatatat gaacattcca aaaatacttg aattaacatt aaataatgga
     2461 tatgacccaa ttagtaaaaa acaaataggt attgaaactg gagaccctag aaatttcaaa
     2521 tcttatgagg aactatttga agcatttaaa aagcaattac attatatgat tgacataaaa
     2581 atagaaggaa atgctgtgat agaaaatata tgtgcaaaac acatgccttg tccacttatg
     2641 tctacaatag ttgatgattg tatagaaaaa ggcaaggatt accaaagagg tggagcaaga
     2701 tacaacacta gatatataca aggtgttggt ataggaacta taacagatag tttaacagca
     2761 ataaaatata atgtatttga taagaaaaaa ttcgatatgg atacattgct taaagcactt
     2821 gatgttaatt ttgaaggata tgaagctata cttaatttag tttcaaataa gactcctaaa
     2881 tatggaaatg atgatgatta tgctgatgaa ataatgcaag agatattcaa tgcatattac
     2941 aatgaggtaa ctggtagacc aactgtttgt ggtggagaat acagagtaga tatgttacca
     3001 acaacttgtc atatatactt tggagaaatt atgggagcaa gtccaaatgg tagactttgt
     3061 gcaaaaccag tttcagaggg aatttcgcct gaaaaaggtg gagatactaa tggacctaca
     3121 gcagttataa aatcttgtgc taagatggac cacataaaaa ctggtggaac attattaaat
     3181 caaagatttg caccatcagt agttcaagga gaaaaagggt tagataatat ggctaatcta
     3241 gtaagagcat actttaatat ggatggtcat catatacaat ttaatgtatt tgataaaaat
     3301 gtactgttag aggctcaaaa gaatcctcag gattataaag acttaatagt tagagttgca
     3361 ggatatagtg accatttcaa taatttaagt agaacactac aagatgagat aataggaaga
     3421 actgagcaaa cattctaggt ttgcttataa ataaaacttc ttactatata aaattgtaac
     3481 tgatataata aagtataaat ggtttgattc catttttaaa attttagatt aattatgagc
     3541 taactttcta aataggaagt tagctcgtta agtttaatga aaaaatcgtt aaatttatat
     3601 ttagtaaatc atactcatat tagtaagtaa tacatacaaa agaataattt attatagtta
     3661 taataaggtt atattttata agaataagat aattttaagc agttaaaata aagtcaattt
     3721 agctacttta aaataaaact actagaaaaa aggagatttg atatgaaaac tttaggattt
     3781 attggttcag ggaatatggg aagtgcaatg ataggtggta tagtacagtc aggattagta
     3841 aaggcagaag atataactgt atcagattta agccaagttg ccttagataa agtaaaagaa
     3901 gaatttggag taaatactac aactgattca aatgtagctg ttacaaattc tgatgtggta
     3961 atagtagcac ttaaacctaa tatatgtgaa aaggctttaa ctccacttaa aggattaata
     4021 gatgtaaata aaataatagt atctatagca gcaggtaaaa ctatagaaag tcttgaaagt
     4081 tttataggtt cagataaaaa aatagtaaga gtaatgccta atactccagc ccttgttggg
     4141 gagggaatgt cagctctttg taaaaatagt aatgtaacag atgaagagtt aaatatgatt
     4201 aaggctctgt ttgaatcttt tggtgaggca gaaatagtat cagaatattt aatggataca
     4261 gttacaggta ttagtggttc ttctcctgca tatgtattta tgtttataga agctatggca
     4321 gatgcagcag tattagctgg aatgccaaga caacaagctt ataaatttgc gtctcaagca
     4381 gtaatggggt cagctaagat ggttcttgaa actggaaaac atccaggtga attaaaagat
     4441 atggtttgtt ctccagcagg aactactata gaagctgtta aggtattaga agaagaaggc
     4501 tttagagcag cagttataaa atctattgtt gcttgtatag ataagtctaa agatatgagc
     4561 aaataacaga tgtaattaag tttactatat ggtttcttga attgcttttt tagtgcttat
     4621 ttatatctat acataaatgt atttggtata agtaaaataa gagtaatttg ctataaaaag
     4681 gaatttaatg attgagtgag aatttattac ttaaatatta ataattaaat agat
//
LOCUS       DAEPXF010000240         4706 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain HB27
            SAMN13376389-rid12479423.denovo.248, whole genome shotgun sequence.
ACCESSION   DAEPXF010000240 DAEPXF010000000
VERSION     DAEPXF010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13376389
            Sequence Read Archive: SRR10518432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4706)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4706)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001069624.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001069624.1
            Genome Coverage       :: 315x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/15/2021 01:54:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,165
            CDSs (total)                      :: 4,076
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 89
            rRNAs                             :: 10, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 10 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 19 of 69
            Pseudo Genes (incomplete)         :: 44 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 10 of 69
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4706
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13376389-rid12479423.denovo.248"
                     /strain="HB27"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="China: Hebei"
                     /collection_date="2017"
     gene            complement(201..401)
                     /locus_tag="KRL67_003564"
     CDS             complement(201..401)
                     /locus_tag="KRL67_003564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003723180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HBH1524400.1"
                     /translation="MNNGIVKWFNNEKGFGFISMEGRDDVFAHFSAIQTSGFKSLEEG
                     QQVSFDIVKGARGPQAENITIL"
     gene            complement(627..803)
                     /locus_tag="KRL67_003565"
     CDS             complement(627..803)
                     /locus_tag="KRL67_003565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH1524401.1"
                     /translation="MFGNKMDKCTHVLTAYISSSYDYCNFLDTQLDDFILEYGENIVE
                     SCLHQVMVLISKYN"
     regulatory      complement(858..955)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            1183..1566
                     /locus_tag="KRL67_003566"
     CDS             1183..1566
                     /locus_tag="KRL67_003566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HBH1524402.1"
                     /translation="MLNKKLPDIELKIMKYIWSTGYKTVISKDVADEIEKTYGWKHTT
                     TITLLVRLTKKDFLISQRIGKHVHYRVLIKEREYLKIEGKRIFGGLHNNPLSELILKL
                     HNEEEITEEKIIEIGSWIKSWKDED"
     gene            complement(1751..2746)
                     /locus_tag="KRL67_003567"
     CDS             complement(1751..2746)
                     /locus_tag="KRL67_003567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HBH1524403.1"
                     /translation="MSIKKKVAIVFTGGTISMTVDDKVGAAIPTLSGEQILSMVTNID
                     KVADVEVFNFDEIPGPHITPYRMMELKNYVNDLLARKDITGVVITHGTDSLEETAYFL
                     DLTIDNIKPVIVTGAMRSSSELGYDGSSNLSASVCTAVSKDAMGKGVLVVLNNEVLLA
                     SEATKTNTLSLNTFKSLTSGPLGIIDCNELVLTRDIVNRTIIDTDKVESKVALFKAYV
                     GENADFIRFAVDSGYKGIVIEAMGRGNIPPQMLSGVEYAREKGLPVVIVSRCHSGRVF
                     DSYGYFGSGRDLKNLGCIFGGDLPGQKARIKLILALGKTDNLDEIKDFFEKGIYC"
     gene            complement(2867..4495)
                     /gene="aspD"
                     /locus_tag="KRL67_003568"
     CDS             complement(2867..4495)
                     /gene="aspD"
                     /locus_tag="KRL67_003568"
                     /EC_number="4.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 4-decarboxylase"
                     /protein_id="HBH1524404.1"
                     /translation="MKNHTYKEIKNIYGKISPFEFKDKLIDIAKYTAKENNRELLDAG
                     RGNPNWTCSTAREAFFTFGHFAITETRSNWDLGHLAGMPQKKGIKERFFKFINENIDM
                     PGAYLARDIINFGISELGFDGDEFVHELADGIIGDNYPLPDRMLPHMEKIVHDYLVQE
                     MKYDISGKYGDVEIFAVEGATAAMCYIFDSLMANELLKKGDTIALMTPIFTPYLEIPN
                     LPRYDFKVVNINANEVDEKGAHTWQYTKKELEKLRDKSIKALFVVNPNNPASIAMDET
                     SCNNLIDVIENYNKDLMIISDDVYGTFVEDFSSLMSKLPYNTVGVYSYSKYFGVTGWR
                     LGTFALHKKNVFDKKINDLTGELKKSVDKRYSDMSLNPSSLSFMERVVADSRLVALNH
                     TAGLSTPQQVQMAFFSAFALIDKVDAYKKLNMSICHRRQKLLFEALELPINENKNNAA
                     YYTQFDIEEWAKLNYGEDLFKFISKNTSPVDVLYKLANDYSVVLLSGNGFYGPEWSIR
                     ISLANLYDEAYTKIGKAIREILNGYIVEWQKIKK"
BASE COUNT         1718 a          726 c          574 g         1688 t
ORIGIN      
        1 ataattatat tgcatatttc ataaatatac attataaagc actcttttta tatgagtgct
       61 tatttttttg aaattaatta atgtataaac tatcaagaac gttactcaat ataccttatt
      121 tacttcatca ataattatct tatatatact aacaataaaa aaaagaccct aaaaagagtc
      181 tctcccgaaa aatctatata ttataatata gttatatttt ctgcttgagg acctctagca
      241 cctttaacta tatcaaagct tacttgttga ccttcttcta atgatttaaa tcctgaagtt
      301 tgtatagctg agaaatgagc aaatacatca tctctacctt ccattgatat aaaaccgaat
      361 cctttttcat tattaaacca tttcactatt ccattattca ttaaaaatac tcctattaac
      421 ctatatcaag atatacttga tttaattgtg tattaccaca aatactatta tatcatagat
      481 taatagaaat gtctacttaa ttatattttt tagttaacat tttagttatt cttaatatta
      541 aaaatacatt taagcgtttt acattgctat atattaatat aaatgtagat aaaactaaaa
      601 agggttctct agaaatcaat gtctatctaa ttatacttac ttatcaatac cattacttgg
      661 tgtaaacaag actctactat attttctcca tactctaata taaaatcatc taactgtgta
      721 tctaaaaaat tacaataatc atatgaactg ctaatataag cagtcaatac atgtgtacat
      781 ttatccattt tatttccaaa catataaatt attatccccc taaaaaactt tcataaaaac
      841 aaaaagaaca atatctcttt ggcaactggc tgacataact cataagatat ttatataagt
      901 cttagtccct atagctttgc gtcactaaat ttctctagtt ttgccgattt agtcttattc
      961 tacaactaaa ataatacaat agaattagtt attattcaac aagattgttt gaaaattgaa
     1021 taaatttaca gatatgaaca taattttaca cttaaaaaac agactaaaca tatagaatac
     1081 ttggtaaaaa tcacctatag tttttgttct aaaaagttcc tattattaat ttaagctaga
     1141 taaatttgaa gaagtgacat aaattgaaag aaggaatata ttatgctaaa taaaaaatta
     1201 ccagatatag aattaaaaat tatgaaatac atatggagta ctggttataa aactgtaata
     1261 tcaaaagatg ttgcagatga aatagaaaaa acatatggct ggaagcatac aactacaatt
     1321 acacttttag taaggctaac taaaaaagat tttttaatat cacagagaat tgggaagcat
     1381 gtacactata gagtattaat aaaagagaga gaatatctca aaatagaggg aaaaagaatt
     1441 tttggaggtt tacataacaa tcctttatca gaattaattt taaaattaca taatgaagaa
     1501 gaaataactg aagaaaaaat aatagaaata ggaagttgga taaaaagctg gaaagatgaa
     1561 gattaataaa taaaaggtag cacttatatt agtaactacc tttttgtgga taatattggg
     1621 gataagttgt ggaaaactta tataaaaatt gtggataaaa tgttttactg acatttaact
     1681 gacataagta ttagattttg atttaaaaat gttgaaatat caataaataa ttaaatagaa
     1741 gctattagaa ttaacaatag attccttttt caaagaagtc tttgatttca tcaaggttat
     1801 cagtttttcc aagtgctaag ataagtttta ttctagcttt ttgacctggc aagtctccac
     1861 caaatataca accaagattt tttaaatctc taccagaacc aaagtaacca taactatcga
     1921 atactctacc tgaatgacat ctagaaacta taactacagg aagtcctttt tctctagcat
     1981 attcaactcc agaaagcatt tgaggaggaa tattacctct acccattgcc tcaataacta
     2041 tacctttata accactatca acagcaaatc ttataaagtc agcattttct cctacatatg
     2101 ctttaaataa agcaacttta gattctacct tatcagtatc tataattgtt ctatttacaa
     2161 tatctctcgt aaggacaagt tcattgcagt caattattcc caatgggcca ctagttagag
     2221 atttaaatgt atttagagaa agagtatttg ttttagtagc ttcagaagct aataatactt
     2281 cattgtttaa aactacaagg actccttttc ccatagcatc tttggatact gcagtacaaa
     2341 ctgatgcaga taaattactt gaaccatcat atccaagctc agaactactt ctcatagcac
     2401 ctgtgactat tacaggtttg atgttgtcaa ttgtaaggtc tagaaaataa gctgtttcct
     2461 ctaaactatc tgtaccatgt gttattacaa cgcctgtaat atcttttctt gctaataaat
     2521 catttacata attttttaat tccatcattc tataaggtgt tatgtgaggt cctggaattt
     2581 catcaaaatt aaaaacttca acatctgcaa ctttatcaat gttggtaacc attgatagta
     2641 tttgttcacc tgataaagtt ggtatagctg ctccgacttt atcatctact gtcatagata
     2701 tagttccacc agtaaaaact atggcaactt ttttcttaat actcatacta ttctctgcct
     2761 ttcataaata aaatttataa ttccgatagg attatatcaa aaaaagtagt taaggtctac
     2821 ataaattctt ttattcatat aatttagata tttcagtgta atataattat ttcttaattt
     2881 tttgccattc aacgatataa ccatttagta tttctcttat ggcttttcct atcttagtat
     2941 aagcttcatc atatagattt gctagagata tcctaattga ccattcaggg ccatagaatc
     3001 catttccact aagtaaaact actgaatagt cattagctaa tttataaaga acatctactg
     3061 gagatgtatt tttacttata aatttaaata agtcttctcc atagtttaat ttagcccatt
     3121 cttcaatatc aaattgagtg taatatgcag cattgttttt gttttcatta attggaagct
     3181 ctaaagcttc aaataatagt ttttgacgtc tatggcagat actcatattg agttttttgt
     3241 aggcatctac tttgtcaatc aatgcaaaag cagagaaaaa cgccatttgt acttgctgtg
     3301 gagtagaaag accagcagta tggttgagag caacaagtct gctatcagca acaactcttt
     3361 ccataaatga aaggctagaa gggttaagac tcatatcact gtaacgctta tctactgatt
     3421 tttttaattc tccagttaaa tcatttattt ttttatcaaa aacatttttc ttatgaaggg
     3481 caaatgtacc aagcctccat cctgtaactc caaaatattt tgaataagaa taaactccaa
     3541 cagtgttgta aggtagtttt gacattaatg atgaaaaatc ttctacaaaa gttccatata
     3601 catcatctga tattatcatt aaatctttat tataattttc aattacatct attagattat
     3661 tacaagatgt ttcatccata gctatagaag caggattatt aggattaaca acgaataaag
     3721 ctttaataga tttgtcacga agtttctcta attctttttt tgtatattgc catgtgtgag
     3781 cacctttttc atcaacttca ttagcattta tatttacaac tttaaagtcg taacgaggaa
     3841 gattaggtat ttcaagataa ggtgtaaata ttggagtcat taaggctatg gtatctcctt
     3901 tttttagaag ttcatttgcc attaatgaat caaatatata gcacatagca gctgtagcac
     3961 cttcaacagc aaaaatttct acatcaccat atttacctga tatatcatat ttcatttctt
     4021 gaactaggta atcgtgtact attttttcca tatgaggtaa cattctatct ggaagtggat
     4081 agttatcacc aataattcca tcagctagtt catgaacaaa ctcatctcca tcaaatccaa
     4141 gttcacttat accaaagttt ataatatctc tagctaaata tgctccaggc atatctatat
     4201 tttcatttat aaatttaaaa aatctttctt taataccctt tttttgaggc attccagcca
     4261 aatgacctaa atcccaatta gaacgagttt cagtaatggc gaaatgacca aaagtaaaaa
     4321 aggcttctct agcagttgaa caagtccagt ttggatttcc acgacctgca tcaagtaatt
     4381 ctctgttatt ttcttttgca gtgtattttg caatatctat taatttgtcc ttaaattcaa
     4441 aagggcttat ttttccatat atgtttttga tttctttata agtatgattt ttcatgttaa
     4501 attcctcctt atgtaataag tttcacattt ttataataaa ataaacaagt tgcaaaaatg
     4561 attatacttt aattataata ggtttataga aaaagtaaag tataaaatta aaataataac
     4621 tctatttaac cttattaaat atacattaaa atgaaaaatg ccttttaata aaaaatgatt
     4681 gttatataaa ataaatattc aattta
//
LOCUS       DAEPXF010000241         4653 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain HB27
            SAMN13376389-rid12479423.denovo.249, whole genome shotgun sequence.
ACCESSION   DAEPXF010000241 DAEPXF010000000
VERSION     DAEPXF010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13376389
            Sequence Read Archive: SRR10518432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4653)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4653)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001069624.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001069624.1
            Genome Coverage       :: 315x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/15/2021 01:54:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,165
            CDSs (total)                      :: 4,076
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 89
            rRNAs                             :: 10, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 10 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 19 of 69
            Pseudo Genes (incomplete)         :: 44 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 10 of 69
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4653
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13376389-rid12479423.denovo.249"
                     /strain="HB27"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="China: Hebei"
                     /collection_date="2017"
     gene            167..1318
                     /locus_tag="KRL67_003569"
     CDS             167..1318
                     /locus_tag="KRL67_003569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HBH1524405.1"
                     /translation="MEIYLDNSATTKPYQEVIDKMVYALNTEYGNPSSVHRKGVEVEK
                     AIKEVRQDIAKSLGAKEKEIYFTSGGTECNNTIIRGITSLNKKRKNHIISTNIEHPSV
                     LNTLKDLEEDGFEVTYLEVGKDGKINIEDLKNAIKSTTCLVSMMHVNNEIGTIQPIGE
                     VGKYLKGLKEKIYFHVDAIQSYGKINFKPSKYNIDFMSVSAHKFHGPKGIGFMYIKEN
                     NRLKPMLTGGGQEIGIRSGTENVPGIYGLGEAVRILNKDLDAVISKVNNLKNILKNEI
                     IDNIEDIKINSPEDGVCHILNVSFRGTKGEVLLHYLEQKGIYVSTGSACSSKKKGSYV
                     LNAIGLTNEEINGTIRFSLSDMNTEEEMLEAVKVLKESICDLRSIMKRK"
     gene            1364..2539
                     /gene="thiI"
                     /locus_tag="KRL67_003570"
     CDS             1364..2539
                     /gene="thiI"
                     /locus_tag="KRL67_003570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 4-thiouridine(8) synthase ThiI"
                     /protein_id="HBH1524406.1"
                     /translation="MVYNILIVKYGEIGVKGKNRYIFENRLIRNIRNMLKPIGRFNVY
                     KEYGRIYVDLEDYDYEEVIEEVRKVFGIVGVCPGVRAKKDYDTLKEIALKMLEEKIEA
                     GYKTFKVESRRGDKSFGLTSQEMSMDIGGYLLSKVGDRINVDVRNPEVKIKCEYREFH
                     TMVYSDTIPGYGGLPLGTNGRAMSLLSGGIDSPVATWMVAKRGMEVEAVHFHSYPFTS
                     ERSQEKVKDLAKILAKYCGRVRLHKVNILEIQKAIGENCNEEEATILSRRFMMRIAQR
                     LSEKRHCDALITGESIGQVASQTIQGLTCTNAVVDLPVFRPLIAMDKSDIVDIAKKIG
                     TFETSIVPEEDCCSVFSPRKPVTKPRLEKIEKSETALDIEKLVQDAIDKIEVEDIEF"
     gene            2695..3498
                     /locus_tag="KRL67_003571"
     CDS             2695..3498
                     /locus_tag="KRL67_003571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HBH1524407.1"
                     /translation="MNKKGFTLIELLVVISIIGILVIVAVPALFRNIEKSKAVTCLSN
                     RENIKTQIVIAMAEEQSKDKNEVIKEVLQNKDGKYFETEPKCKSGGIYSATFDDGYDG
                     ITGIESIAKVYVTCTKHPDGVEMARDVHQSMKDLIASFAQDPSIIPGASKGNDDFRKY
                     LLDNKYKNGWPTIPDEFKAKYGLSKDTLYIQPYAYNPTKSDATVVVFANNKTGGNWYT
                     SLVYDYDEGRWYKGKNGISVAGRSWDVDTDSAKSVKTEIHSKEGWGPLN"
     gene            3739..4380
                     /locus_tag="KRL67_003572"
     CDS             3739..4380
                     /locus_tag="KRL67_003572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase"
                     /protein_id="HBH1524408.1"
                     /translation="MNNNFQDNQTINLIQSRRSIRKFTTEQISDEQVNTLLHCAFAAP
                     SGCNKQPWHITVVQDQELLKEISDDTLSRIHEVSNVEINKNFKLFYGAPTVLFISYDE
                     SSSWAPYDIGILTGNITTAAQALGLGSCIIGMVRGLFTPVEQGDIEGLVSVLDKEDVK
                     ESESIKMKFDTNKKYRELLDIPEGYSVPFGIAVGVPDGNLPNAREVVYKVSRV"
BASE COUNT         1858 a          450 c          902 g         1443 t
ORIGIN      
        1 tattttacta tgaaaatttg taataaagtg aaaaaactag cttttcatga aataatattt
       61 tataatttaa gattgtcttt ttaataaaga ttaacaaaaa aaatactata attagtggta
      121 taatagttag agttgaagat tttaaataaa ggtaggtgta attaagttgg aaatatactt
      181 agacaatagc gcaactacaa aaccttatca agaagttata gataaaatgg tttatgcact
      241 taatacggaa tatggaaatc cttcttctgt ccatagaaaa ggtgtagaag ttgaaaaagc
      301 tataaaggaa gtaagacaag atatagctaa atctctagga gcgaaagaaa aggagattta
      361 ttttacatca ggtggtacag agtgtaataa tactataata agaggtatta ctagtttaaa
      421 taaaaaaaga aaaaatcaca taatatctac taacatagaa catccttctg ttttaaatac
      481 attaaaagat ttagaagaag atggttttga agtaacttat ttagaggtcg gaaaagatgg
      541 gaaaataaat atagaggatt taaaaaatgc gataaagtca actacatgct tagtaagtat
      601 gatgcatgtt aacaatgaaa tagggactat acagccaata ggtgaagttg gaaagtattt
      661 gaaaggttta aaagaaaaga tttatttcca tgttgatgct attcagtcat atggaaaaat
      721 taactttaaa ccatctaaat acaatataga ttttatgagc gtaagtgcac ataaattcca
      781 tggtccaaag ggaattggat ttatgtatat aaaagaaaat aatcgcctta agcctatgtt
      841 aacaggtggt ggtcaggaaa ttggaattag gtcgggaaca gaaaatgtac ctgggatata
      901 tggtcttgga gaagctgtta gaatactaaa taaagattta gatgctgtca tttcgaaagt
      961 aaataactta aaaaatatat taaaaaatga aataattgat aatatagaag acataaaaat
     1021 taattctcca gaagatggag tatgtcatat attaaatgta tcatttagag ggacaaaagg
     1081 agaagtttta ctccattact tagagcaaaa aggtatttat gtatcaactg gctctgcatg
     1141 ttcctctaaa aagaaaggta gttacgtatt aaatgctata ggtcttacaa atgaagaaat
     1201 aaatggaaca ataagattta gtttatctga tatgaataca gaagaagaaa tgttagaagc
     1261 tgtgaaagtt ttgaaagaat caatttgtga cttaaggtca ataatgaaaa gaaaataaag
     1321 aatactgtat aaatagtgat aataaattag tttaagaggt gaaatggtgt ataatatatt
     1381 aatcgtaaag tatggagaaa ttggagtaaa aggaaagaat agatatatat ttgagaatag
     1441 gcttataaga aatataagaa atatgttaaa accaattgga agatttaatg tatataaaga
     1501 gtatggaaga atatatgttg atttagaaga ctatgattat gaagaagtaa ttgaagaagt
     1561 tagaaaagta tttgggatag tgggtgtgtg ccctggagta agagctaaaa aagattatga
     1621 tacattaaaa gaaatagctt taaaaatgtt agaagaaaaa atagaggctg gatataaaac
     1681 ttttaaggta gagtcaagaa gaggagataa gtctttcggg ttgacttctc aagaaatgag
     1741 tatggatata ggtggatact tattatcaaa ggtaggagat aggattaatg ttgatgtaag
     1801 aaacccagaa gttaaaataa agtgcgaata cagagaattt catactatgg tctatagtga
     1861 tactatacct ggatatggtg gattgccact tggaacgaat ggtagagcta tgtctctttt
     1921 atcaggaggt atagatagtc cagtagctac atggatggtt gcaaaaagag gtatggaagt
     1981 agaggcagtt cattttcata gttatccatt tacaagtgaa aggtcacagg aaaaagttaa
     2041 ggatttagct aaaatactgg caaaatactg tggtagagta aggcttcata aagttaatat
     2101 cttagaaata caaaaagcaa ttggcgagaa ttgtaatgaa gaagaagcta ctattctttc
     2161 aagaagattt atgatgagga tagcacagag actttctgaa aaaagacatt gtgatgcttt
     2221 aattacaggt gaaagtattg ggcaggttgc atctcaaact atacaaggac ttacttgtac
     2281 taatgccgtt gtagacttac cagtgtttag accacttata gcaatggata aatcagatat
     2341 agttgatata gccaaaaaaa tagggacttt tgaaacatct atagtgcctg aagaagattg
     2401 ttgtagtgta ttttctccta gaaagcccgt cactaaacct agattagaga aaatagaaaa
     2461 atctgaaaca gcacttgaca ttgaaaaatt agtacaggat gctattgaca aaatagaggt
     2521 agaggatatt gagttttagc atacgagtta tttaattatt aaattgaata ttgagtatat
     2581 tagtagttct gtaagtatat tagtagttct gttgaaagga tttatgtaga caatttatta
     2641 atatgtagta tttaaaaata agtactataa ttttatttaa tgggaatgat tattatgaat
     2701 aaaaagggtt ttacactaat tgaattgtta gtagttatat ctataatagg cattttagtt
     2761 atagtagctg ttccagcatt gtttagaaat atagagaaaa gtaaagcagt tacatgtctt
     2821 tctaatagag aaaatataaa gactcaaatt gttattgcaa tggcagagga acaaagtaaa
     2881 gacaagaatg aagtcataaa agaggtatta caaaacaaag atggtaagta ctttgaaaca
     2941 gaaccaaagt gtaagtcagg tggaatatat tcagcaacgt ttgatgatgg ttatgatgga
     3001 ataactggaa tagaaagcat tgcaaaagtg tatgttactt gtacaaaaca tccagatggt
     3061 gttgaaatgg ctagggatgt acatcaaagt atgaaagatt tgattgcatc atttgcacaa
     3121 gacccttcta taataccagg agcttcaaag ggtaatgatg attttagaaa atatttatta
     3181 gacaataaat ataaaaatgg gtggcctaca attccagatg aatttaaggc aaaatatgga
     3241 ttaagtaaag atacactata tatacaacca tatgcatata atcctactaa atctgatgct
     3301 actgtagttg tatttgcaaa taataagact ggaggtaatt ggtatacttc tctagtttac
     3361 gattatgatg aaggtagatg gtataaaggt aaaaatggta tttctgttgc aggtaggtca
     3421 tgggatgttg acacagatag tgctaagtct gtaaaaacag agattcattc taaagaggga
     3481 tggggtcctt taaattaata taacatgttt tattaatctg gtattgatta aactatggaa
     3541 aatagcagtt tctatagatg acgaatacca gatttttatt tatatataat taagatatat
     3601 taaaagtgtt tatattttta agtcttcaca tacaagctaa aaataattat aataatattg
     3661 taaagaaatt taattataag attaattttc tgaaaattta gtatttgtac taagaaatac
     3721 ataaaaaggg gaatagatat gaataataat tttcaagaca accaaacaat aaatttaata
     3781 caaagtcgta gaagtatacg aaaatttaca acagaacaaa ttagtgatga gcaagtaaac
     3841 acattactac attgtgcatt tgcagcacct tcaggatgta ataaacagcc ttggcacata
     3901 actgttgtgc aagaccagga acttttaaaa gagattagtg atgatacttt atctaggatt
     3961 catgaagtga gtaatgttga gataaataaa aactttaagt tattttatgg tgctccaacc
     4021 gttttattta tttcttatga tgaaagtagt tcatgggctc catatgatat aggaatactt
     4081 acagggaata taacaacagc tgcacaagca ctagggcttg gaagctgtat aataggaatg
     4141 gttagaggtt tatttacacc tgttgaacaa ggggatattg aaggtcttgt gagtgtattg
     4201 gataaagaag atgtaaaaga gtcagagtca ataaagatga agtttgatac aaataaaaaa
     4261 tacagagaac tacttgatat accagaaggg tattctgtac catttggtat tgctgtaggt
     4321 gttccagatg gaaatttacc aaatgctaga gaggtggttt ataaagtttc tagagtttag
     4381 gataaagtat tgcaacaact aatcagttac ttgaagaatt aaatcagtaa tatacaaata
     4441 aattaatgta agaggaagct atttaaaaag taatttagat ttttcattgt aaaataatta
     4501 aataaagaat aaggtgataa gtattttatt tattatctta ttctttttta tttttgtttt
     4561 gtagaatgat aaaaataagg attaagtgta ataaaataga aaacaagtat gttaatttta
     4621 agtatgtgct ttaaagttta tccaagttaa aaa
//
LOCUS       DAEPXF010000242         4561 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain HB27
            SAMN13376389-rid12479423.denovo.250, whole genome shotgun sequence.
ACCESSION   DAEPXF010000242 DAEPXF010000000
VERSION     DAEPXF010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13376389
            Sequence Read Archive: SRR10518432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4561)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4561)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001069624.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001069624.1
            Genome Coverage       :: 315x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/15/2021 01:54:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,165
            CDSs (total)                      :: 4,076
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 89
            rRNAs                             :: 10, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 10 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 19 of 69
            Pseudo Genes (incomplete)         :: 44 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 10 of 69
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4561
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13376389-rid12479423.denovo.250"
                     /strain="HB27"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="China: Hebei"
                     /collection_date="2017"
     gene            237..1211
                     /locus_tag="KRL67_003573"
     CDS             237..1211
                     /locus_tag="KRL67_003573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH1524409.1"
                     /translation="MNNINSLKVIKLSLGCSLAIFIAWLLKLEYSMVAGVIVLLTVKD
                     TKRETLKGSIGKIYGFLLCTIFSYLCFNILGYNLTSFSIFIFIIIPLCFLLNIQDVIA
                     MCVVISSHYYLQGETSIKWILNEAGIFAVGTGIGVLINMYIPTNIHKIYEVQKKLQKE
                     VSIVLIDIADIIVNPKKENGYSRDLYTLNSLIDSSISETYDNINNTLLSDTRFFLEHM
                     NIIKSQRDILENLYSYVSQLNSTPPQAHILSAFIHKIGYTEFEAETGNLLLEELKRLM
                     LSMKNQPLPVDRTEFENRAILFLCLTELKQFLVNRKHAQMLRDNNFYK"
     gene            complement(1327..2373)
                     /locus_tag="KRL67_003574"
     CDS             complement(1327..2373)
                     /locus_tag="KRL67_003574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
                     /protein_id="HBH1524410.1"
                     /translation="MKKIRKLATKLLITTIVLVSGMSMTVYGMTAKEVTAKTPKSYVT
                     GTNSVYGPKLSQAQLNSVAQATADFMNNKITKNMTTDAKILVAYNHIKNNTTYVDWNA
                     VEGANTAYTLVTKKGACSGMARSMKALCDAMGIESYYVHSTSNDHQWNLIRFGDGRLY
                     HVDIDANKSAGKDVIYKSLSHPLPFDKTAYPTVGAVAQTPKKPAPPGKHTMLPHQEGS
                     KVVGNFGKNGEDINEKHVMYEGVRCSVVNSSVNTITFTGSESNLDPSNLTIAVIGFMN
                     TPHGMFALAPAGKPVEYPFKYDKPFSLDFSGKNRSGAELAKYCKKNDVTIKIEITDEK
                     ANTGLGQIMYVRYE"
     gene            complement(2984..3835)
                     /locus_tag="KRL67_003575"
     CDS             complement(2984..3835)
                     /locus_tag="KRL67_003575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HBH1524411.1"
                     /translation="MSCILKLKQIYEDLTEVDKKIADYILNNAEAISKLSVSELASNS
                     KTSTASIVRFSRKMGYSGFGDLKIEIAKDLMGKENEYTYVDENYDCNIDSVINKITNK
                     NIETINQTRLLNEKDIIKEAVEQIIKAKNVYIFGVGGSALVALDLQMKLLRINKQAFT
                     SLDSHTQLMVSSNVDKEDIAIAISYSGESKEVIKSIENAKLKGCKVICITKYSDNHLS
                     KISDLKLVVPNIEKRLREGAISSRIAMLTLIDIIYISIIQENLNEAEQKLQETKRILD
                     YLNIDNA"
     gene            complement(3852..3989)
                     /locus_tag="KRL67_003576"
     CDS             complement(3852..3989)
                     /locus_tag="KRL67_003576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH1524412.1"
                     /translation="MFKSGDLILESLKKYLSKARFDIVRPVLTPDLGACLLAKKKYYS
                     K"
     gene            complement(4197..>4561)
                     /locus_tag="KRL67_003577"
     CDS             complement(4197..>4561)
                     /locus_tag="KRL67_003577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBH1524413.1"
                     /translation="QKLSTKLIKENDIICIEDLQVKNMIRNRKLSRLISDVPWSEFIR
                     QLEYKANWYGRQIVKVGKFFASSQICNKCGYKNEEVKNLNIREWICPSCNETHDRDIN
                     ASINILKEGLRLITIQNK"
BASE COUNT         1446 a          754 c          583 g         1778 t
ORIGIN      
        1 tttcatttgc tacttaaaag caattttaag ttttcttcct ataataaact tcctctttat
       61 ggtctcatgt tagtctctaa atttaaaatt ttacaaaaaa ttttcataaa ataaaattat
      121 attgattgta tctaaacaaa tagggcaata aaataattaa gtatagttac ttacctttca
      181 agcaatatta gttttatgtt agaattttat tatattgatt tacaggaggt ttacatttga
      241 ataatattaa ttcacttaaa gtaataaaat tatctcttgg atgttctctt gctatattta
      301 ttgcttggct attaaaatta gaatactcaa tggttgctgg agttatagta ctacttacag
      361 taaaggacac taaaagagaa actcttaaag gctccattgg aaagatttat ggttttcttt
      421 tatgtactat tttttcatat ttatgtttta atattttagg atataatttg acttcatttt
      481 caatatttat atttataatt ataccacttt gttttttgtt aaatattcaa gatgttattg
      541 ctatgtgtgt agtcatatca tcccattact accttcaagg agaaacttct atcaaatgga
      601 ttttaaatga agctggaata tttgcagtag gaactggaat aggtgtctta ataaatatgt
      661 atattccaac aaatatacat aaaatctatg aagttcaaaa aaaattacag aaagaagtga
      721 gtattgtact tatagacatt gcagatatta ttgtaaatcc taaaaaagaa aatggatata
      781 gtagagattt atatacatta aattcactta ttgacagcag tatttctgaa acttacgata
      841 atattaataa tactttatta agtgatacta gattcttctt agagcatatg aatattataa
      901 aaagccaacg ggatatctta gaaaatttgt atagttatgt atctcaacta aattctacac
      961 caccacaagc tcatattcta tctgctttta tacataagat aggttatact gaatttgaag
     1021 ctgaaacagg caatcttctt cttgaagaat taaagagact aatgcttagt atgaaaaacc
     1081 agcctctacc tgttgacaga acagagtttg aaaatagagc tattttattc ctatgcttga
     1141 ctgagttaaa acagttttta gttaatcgta aacatgctca gatgcttaga gataataatt
     1201 tttataaata aaaaggggag aaatccagca gttgatctga ctacaaaata tcagatataa
     1261 tattaaatgc gcccctagta agagggcgca atgtttataa cttattctac ggtcatgatt
     1321 aaatcattat tcatatcgaa catacattat ttgaccaagt ccagtattag ctttttcatc
     1381 agtgatttct attttaatag tcacatcatt ttttttgcag tacttcgcta attctgcacc
     1441 agaacgattt ttaccactaa aatctaaaga aaatggctta tcatatttaa aaggatattc
     1501 aacaggttta cccgctggtg ctaatgcaaa catcccatgt ggtgtgttca taaatcctat
     1561 tacagcaatc gtaagatttg atgggtctaa atttgattca ctacctgtaa atgtaattgt
     1621 atttactgat gaattaacta cactacatcg aacaccttca tacattacat gtttttcatt
     1681 tatatcttct ccattttttc caaaatttcc aacaaccttt gacccttctt gatgtggtaa
     1741 catagtgtgc ttcccaggtg gtgctggttt ttttggtgtt tgtgctactg ctccaactgt
     1801 tggatatgct gtcttatcaa atggtaatgg gtggctaaga ctcttgtata tgacatcctt
     1861 tcctgctgat ttattagcat caatatctac atgatacaaa cgcccatcac caaaacggat
     1921 aagattccac tgatggtcat ttgatgtgct gtggacataa taagattcaa ttcccatagc
     1981 gtcacacaaa gctttcatgc tcctagccat tcctgaacat gcaccttttt tagtaaccaa
     2041 tgtataggca gtattcgctc cttctactgc attccagtca acatatgttg tgttattttt
     2101 aatgtgatta taggcaacta aaatcttagc atctgtagtc atattctttg tgattttatt
     2161 gttcataaaa tctgctgttg cttgtgctac tgagtttagt tgggcttgtg acagctttgg
     2221 accatatacg ctattggttc cagtgacata gctttttggt gtttttgctg ttacctcttt
     2281 ggctgtcata ccataaactg tcatactcat acctgagact aaaacaattg ttgtaatcaa
     2341 taacttggta gctaacttcc taattttctt catacttagt attctccttt ataaaatatt
     2401 gttacttcct attctctacc attaataaag ttcatttggg atttaaaaag gtggattgtc
     2461 aatatgtatg tttcttcatt tatacgttcg tgaaatgttt aaatttcatg gaaaatctaa
     2521 tagatatttt ctagataatt tgtatacaat acaactcgca aatcagtatg tttgctgtta
     2581 gtttgagaaa atgatgatga ttaatatatg cggaatcttt gacctataat aaatactact
     2641 gtcttcaatc attaataatg gtaagagtaa agagaattat caataataca gttaaaatca
     2701 actttgtata catttcatac attctctctc cacaacatta tacctcctat aatttgtata
     2761 ttcaatacat tttttaggaa aatctaggat atatctgtaa gaatataaaa aaggagttga
     2821 gcttaaaatt tgattaaaaa aattaatttt aagacaactc atttgtatta taaaaatgct
     2881 atatattatt attgagtaag tcaatatttt agatatttat ctatttctta ttgacttacg
     2941 cttatagtct aacttatttg tttagttgat atataatcta gttttaagca ttatcaatat
     3001 ttaaataatc aagtatcctt tttgtttctt gtagtttctg ttctgcctca tttaagtttt
     3061 cttgaattat agaaatatat attatatcta tcaacgtaag cattgctatt cttgaagaaa
     3121 tagctccttc tctaagcctt ttttctatat ttggtactac tagctttaaa tcactaatct
     3181 tactaagatg attgtcacta tatttagtta tacaaatgac cttacatcct tttaattttg
     3241 cattttctat agactttatt acttcttttg attctccaga gtaagatatt gctatagcta
     3301 tatcttcttt atctacattt gaagatacca taagttgtgt atgactatct aaagaggtaa
     3361 atgcctgttt atttatcctt aaaagtttca tctgtaaatc tagtgcaact aaagccgaac
     3421 cacctactcc aaatatatat acatttttag cctttattat ctgttcaact gcttctttta
     3481 ttatatcttt ttcattcaac aaccttgttt gattgatagt ctctatgttt ttgtttgtaa
     3541 ttttgtttat aacactatct atattacaat catagttttc atctacatat gtgtattcat
     3601 tttctttgcc catcaaatct ttagctatct caatttttaa atccccaaaa cccgaatatc
     3661 ccatttttct agaaaaccta actatacttg ctgtactcgt ttttgagttt gatgccaatt
     3721 ctgatacaga aagcttactt atagcctctg cattattaag tatgtaatca gctatttttt
     3781 tatctacttc cgttaaatct tcatatatct gttttaattt taaaatgcaa ctcataccct
     3841 accacctcta cctatttact ataatatttc ttcttagcaa gtaaacatgc tcctaaatct
     3901 ggagtcaaca ctggtctcac tatatcaaat ctcgctttac tcaaatattt tttaagtgat
     3961 tctaatatca aatctcctga cttaaacact ccacctgaat aagatacctt tatctttgta
     4021 ccttgttcaa aatgatgctc taatcctatt ataagcctac ttaattcata agctgcatcc
     4081 tgaacctccc atgactaaag tcacagggtt cctgcttcat agaattttgc atactaaaat
     4141 atgtcttact aattctccac aggctatccc cgtagttcct acggttctta cataagttat
     4201 ttattttgaa ttgttattag tcttaatcct tcttttaata tatttatact tgcatttata
     4261 tctctatcgt gagtttcatt acaactagga caaatccatt ctcttatatt taagttcttc
     4321 acttcctcgt ttttgtaccc acatttattg catatttgtg aacttgcaaa gaatttaccc
     4381 acctttacaa tttgtctacc ataccaatta gccttgtatt ctaattgacg aataaactct
     4441 gaccatggca catcagaaat taagcgagat agtttacgat ttctaatcat attctttact
     4501 tgtaaatctt ctatgcaaat aatatcattc tcttttatta atttagtaga tagtttttgt
     4561 a
//
LOCUS       DAEPXF010000243         4490 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain HB27
            SAMN13376389-rid12479423.denovo.251, whole genome shotgun sequence.
ACCESSION   DAEPXF010000243 DAEPXF010000000
VERSION     DAEPXF010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13376389
            Sequence Read Archive: SRR10518432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4490)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4490)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001069624.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001069624.1
            Genome Coverage       :: 315x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/15/2021 01:54:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,165
            CDSs (total)                      :: 4,076
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 89
            rRNAs                             :: 10, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 10 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 19 of 69
            Pseudo Genes (incomplete)         :: 44 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 10 of 69
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4490
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13376389-rid12479423.denovo.251"
                     /strain="HB27"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="China: Hebei"
                     /collection_date="2017"
     gene            32..565
                     /locus_tag="KRL67_003578"
     CDS             32..565
                     /locus_tag="KRL67_003578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HBH1524414.1"
                     /translation="MTEVIIVNDARIISQIKAGKKELFDTLIKKYYDSIYCYCYRHVD
                     NKMTAQDLTQDVFVTVIKNIGNYKHYGKFENYLYVIAKNKCKDFWKKKQPIYLESLDK
                     EISNQELSDIENKMFIKEALTKLPEVEFEVIILRYYHDLKFKDIAKIMESSVSVTKYR
                     MSRALKKLELYLEGGKL"
     gene            562..1323
                     /locus_tag="KRL67_003579"
     CDS             562..1323
                     /locus_tag="KRL67_003579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH1524415.1"
                     /translation="MNNKEIKRSLQKYKVPKYDSSALEKTIKTIHELTLNNTDKRMNN
                     KDFFISQIGFIRKRTWIMQFLILCTICILLFSTSTDSNSFYNTLSLISIASPLFVLTN
                     IEELTKVYNSSILEIEFSTKNTLHKIIATRMLIFGISDLLFMIIILVSAKQIIDVNIL
                     YIIIYILVPFNLMCIGCMEIINRNKGKHTNYYCMIYGISLVLLIVTLSNFRDNLYQFE
                     AINQWLIVFLVTAITLIYEIRKTWRHLCSFENIII"
     gene            1361..2227
                     /locus_tag="KRL67_003580"
     CDS             1361..2227
                     /locus_tag="KRL67_003580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBH1524416.1"
                     /translation="MELSVERLTKQYNNKIIVDKIDFKLNEGIYGFLGENGAGKTTLM
                     RMICGILSPSSGEVKVDGKNNLDMGEDFRNMIGYLPQNFGYYPDFTAKEFMLYIASLK
                     GLMPSYAKAKTQELLEMVGLSGVINKKIRTFSGGMKQRLGFAQTLLNDPKILILDEPT
                     AGLDPKERVRFRNLISEFSKEKIVILSTHIVSDVEYIADKILIMKKGRFILTGSVEEV
                     TSKIKDKVWKCVVNENEIDYMSDRYCLVNIHHSGGVANLRIVSDVKPYDNACNLKPTL
                     EDLYLYCFNEVL"
     gene            2248..3489
                     /locus_tag="KRL67_003581"
     CDS             2248..3489
                     /locus_tag="KRL67_003581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBH1524417.1"
                     /translation="MSTLIKFELKKIIKRKSTIAVILGSLFLSIILFALMPIQEKAND
                     ESGKEYRGLQAIELKKQQSKLNDGYITDKKVENSIREYQKMFNNPNNITTDEGGFSNF
                     KPDIYWKFFYPNKAFYNFIIESYSNPNDYNPEIIKSMPLEDGVNFYKFRNEKVNTILN
                     MEYPDKNYTQKEKDFWLSKNKDIKEPYLNGYYQGWNYFLLARDLLIFTIVAVCISIAS
                     VFSGEYQSNADSIILSSKYGKTKVITAKIVSSFLFGTIIFTINTIVSIGILLLSFGTD
                     GWNLPLQLTTSSIPYPYTFLQAILLSVLTIYMILIVLISFTLLLSSYMKSPFPVLITN
                     IILLFVPIFFKYSETNKLYNQILRILPSNAISFSFTDYIDYKFGNLIVTLPTMIIFTC
                     LVLTVVFLPFVKNNFKEHQVN"
     gene            complement(3707..4285)
                     /locus_tag="KRL67_003582"
     CDS             complement(3707..4285)
                     /locus_tag="KRL67_003582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBH1524418.1"
                     /translation="MFKKIVAIASSIIVSTICLTGCDFNSNDKKDESSSSKNLDKVTI
                     AEVTHSVFYAPQYAAITKGFFEDEGIKVDLINTQGADKTMAALISGEANVGLMGPEAS
                     IYVYNQNKNDYAVNFAQLTKRDGSFLIARKKMPNFSYNDLKGTEILGGRKGGVPLMTF
                     EYVLKEKGLTIGENLKAGNVNVRTDVQKSVCL"
BASE COUNT         1744 a          523 c          645 g         1578 t
ORIGIN      
        1 gactttttta tagtataaat cgttatatat aatgacggag gtgataatag tgaatgatgc
       61 tcggattata tcacaaataa aagcaggaaa aaaagaattg tttgatactc taataaaaaa
      121 atactatgat agcatctact gttattgtta tcgccatgta gacaataaaa tgactgcaca
      181 agatttaact caagatgtat ttgtaacagt tataaagaat ataggaaatt ataaacatta
      241 tggtaagttt gaaaactatt tgtatgttat tgcaaaaaac aagtgtaagg atttttggaa
      301 gaaaaaacaa ccaatatatt tagagtcatt agataaggag atatcaaatc aagagttatc
      361 tgatatagaa aataaaatgt ttataaaaga agctctgact aaattaccag aagtagaatt
      421 tgaagtgata attttacggt attaccatga tttaaaattt aaggatattg ctaaaattat
      481 ggagtcaagt gtctctgtca caaagtacag gatgagtagg gcattaaaaa aactagaatt
      541 atatctggaa ggaggtaaat tatgaataat aaagaaataa aaagaagtct acagaagtat
      601 aaggttccaa aatatgattc ttcagctctt gaaaagacta ttaaaactat acatgagcta
      661 acacttaata atactgataa aagaatgaat aataaagact tttttatatc tcaaattggt
      721 tttataagaa aaagaacttg gattatgcaa tttctaatac tatgtacaat atgtatactt
      781 ttatttagta catcaacaga tagtaattca ttttataata ctttatcctt gatttctata
      841 gcatcaccac tttttgtact gacaaacata gaagaattaa caaaagtata taatagtagt
      901 attttagaga tagaattttc aacaaaaaat acattacata aaattattgc tactagaatg
      961 cttatatttg gaataagtga tttattgttt atgattatta ttttggtatc tgcaaaacaa
     1021 atcattgatg taaacattct gtatataata atttatatac tagttccttt taacttaatg
     1081 tgtataggat gtatggaaat tataaataga aataaaggca agcatacaaa ttattattgt
     1141 atgatatatg gtatatctct tgtattatta atagttacac tgagtaactt tagagataat
     1201 ctatatcaat ttgaagcaat aaatcaatgg ctaattgttt ttctagtcac agcaataaca
     1261 ttaatatatg aaataagaaa aacgtggaga catctttgtt catttgaaaa tattattatc
     1321 taagtaaaaa ataagaatac acagtatcag gaggaatagt atggagttaa gtgtagaacg
     1381 tttaacaaaa cagtataaca ataaaattat agtagataaa attgacttta aattaaatga
     1441 gggtatatat ggatttttag gagaaaatgg ggcaggaaaa acaactctta tgagaatgat
     1501 ttgcggaata ttaagccctt catctggaga agtaaaggtg gatggtaaaa ataatttgga
     1561 tatgggagaa gattttagaa atatgattgg ataccttcct caaaactttg gatactaccc
     1621 agattttact gctaaagaat ttatgctata tatagcatca ttaaaaggat taatgcctag
     1681 ctatgcaaag gcaaaaacac aagaattatt agaaatggta ggtttatcag gagtaattaa
     1741 taagaaaata agaacatttt ctggaggtat gaagcaaagg ctaggatttg ctcaaacatt
     1801 acttaacgac cctaaaattt taatattaga tgagccaaca gcagggctag accctaaaga
     1861 acgagttaga tttcgcaact taatttcaga attttctaag gaaaagattg ttatcttgtc
     1921 tactcatatt gtgtctgatg tagaatatat tgcagataaa attttaataa tgaaaaaagg
     1981 aagatttatt ttaactggct cagtagaaga agtaacttca aagataaaag ataaagtatg
     2041 gaaatgtgtg gttaatgaga atgaaataga ttatatgtct gatagatatt gtttagtaaa
     2101 tatccatcat tctggtggag ttgcaaatct tagaattgta agtgatgtaa agccatatga
     2161 caatgcatgc aatttaaaac caacattaga agatttgtat ttatattgtt tcaatgaagt
     2221 actataaata tacataagga gataggtatg tctacattaa ttaaatttga gttaaaaaaa
     2281 ataataaaaa gaaagtctac aatagcagtt attttaggaa gtttattctt aagcatcatt
     2341 ctttttgcat taatgccaat ccaggaaaaa gccaatgatg aaagtggaaa agagtatagg
     2401 gggctacagg ctattgagtt aaaaaaacaa caatctaaat taaatgatgg atatataaca
     2461 gataaaaaag tagaaaactc aatcagagaa tatcaaaaaa tgtttaataa tccaaataat
     2521 attacaacag atgaaggtgg gttttcaaat tttaaaccag atatttattg gaaattcttc
     2581 tatccaaata aagcttttta taattttata atagagtcat atagtaatcc aaatgattat
     2641 aacccagaaa taattaaatc aatgccctta gaagatggag ttaattttta taaatttaga
     2701 aatgaaaaag taaatacaat attaaatatg gaatatcctg ataaaaatta cacacagaaa
     2761 gagaaagatt tttggttatc taaaaataag gatataaagg agccttattt aaatggatat
     2821 tatcaaggat ggaactattt tctattggct agagatttac taatttttac tatagtagct
     2881 gtctgcataa gtatagcttc tgtattttca ggagaatacc aatccaatgc tgactcaata
     2941 atattatctt ctaaatatgg aaaaacaaaa gtcataacag ctaaaatagt aagttccttt
     3001 ttatttggta cgattatttt tacaataaat acaattgtat ctattggcat acttctttta
     3061 tcttttggaa ctgatggctg gaatttacca ctacaactta caactagttc aattccatac
     3121 ccatatacat ttttacaggc aatattacta agtgtgttaa ctatctatat gatattaata
     3181 gtattaatta gttttacact tttattatcg tcttatatga aatctccttt tccagtttta
     3241 attacaaata taatattgtt atttgtacca atatttttca aatacagtga aacaaataaa
     3301 ttgtacaatc agattctcag aattttacca tcaaatgcta tttcattttc atttactgat
     3361 tatatagatt ataaatttgg taatttaata gtaactttac ctacaatgat tatttttaca
     3421 tgcttggtgc ttacagttgt ttttttacct tttgttaaaa ataattttaa agaacatcaa
     3481 gttaactaaa atatatatta taaaaagaag gtgtcttaaa atgaattttt tagcttgcta
     3541 ggcactcttc tttttattaa tttaaatata ttttcgtatt aaattaaata tgttttcatc
     3601 tttttaacaa tgaaaaagag ccttatctct attttgagat agctcttata aagttaaata
     3661 ttgaattgat aactataaaa tatgtagttt aaatttataa tttatgttat aaacaaactg
     3721 atttttgtac atcagttctt acattaacat tacctgcttt tagattttct ccaatagtaa
     3781 gacctttttc ttttagaaca tattcaaaag tcattagtgg aactccacct tttctaccac
     3841 ctaatatttc tgttcctttt aaatcattgt aagagaaatt aggcattttt ttcctagcaa
     3901 ttaaaaaact accatctctt tttgttaatt gagcaaagtt cacggcataa tcgtttttgt
     3961 tttgattata tacatatata gatgcttcag ggcccattaa acctacgttt gcttcacctg
     4021 aaatcaaagc tgccatagtt ttatctgccc cttgggtatt tataaggtca acctttatac
     4081 cttcatcttc aaagaatcct ttcgtaatag cagcatactg tggagcataa aaaacagagt
     4141 gagtaacttc tgctatagtt actttatcca gatttttaga cgaagaagat tcatcttttt
     4201 tgtcattaga gttaaaatca catccagtta gacatattgt acttacaatg atagagcttg
     4261 ctattgcgac tattttttta aacataataa atacctccca taagacatac gaataatata
     4321 tagtattaaa tatttattgt agttgttaca aaaatgtatc tatgcttata atatttctac
     4381 aaaaacttaa taataatttt cagttttatg tttagttttt ttagtaatgt gtaatattgg
     4441 ataaattata ttgattattt ataaactaag taataaaatt aaaataatat
//
LOCUS       DAEPXF010000244         4477 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain HB27
            SAMN13376389-rid12479423.denovo.252, whole genome shotgun sequence.
ACCESSION   DAEPXF010000244 DAEPXF010000000
VERSION     DAEPXF010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13376389
            Sequence Read Archive: SRR10518432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4477)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4477)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001069624.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001069624.1
            Genome Coverage       :: 315x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/15/2021 01:54:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,165
            CDSs (total)                      :: 4,076
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 89
            rRNAs                             :: 10, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 10 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 19 of 69
            Pseudo Genes (incomplete)         :: 44 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 10 of 69
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4477
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13376389-rid12479423.denovo.252"
                     /strain="HB27"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="China: Hebei"
                     /collection_date="2017"
     gene            34..2868
                     /locus_tag="KRL67_003583"
     CDS             34..2868
                     /locus_tag="KRL67_003583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBH1524419.1"
                     /translation="MLRKDKVLLSLHKLCENMTLKDLQSDKTGFTTIEIANHSSLDRS
                     NTSKELNNLYNEKKVLKISGKPILFLHASIIENLIGRTLNSDEFSINNCNELLKSSKN
                     DSEDIFSTLLGHDSSLKLPIEKAKSAILYPPKGLNTLLIGPTGVGKSTFAETMYKYAV
                     ESKIFSSESKFVVFNCAEYAENANLLLSNLFGYVKGSFTGANKDKFGIVAQADGGILF
                     LDEIHRLPPEGQEMLFLLMDKGIYRRLGESDIVHKANVFIVCATTEDIQSSLLGTFLR
                     RIPVTINLPALQDRSLKERLSLIKYFFIIESKYTNATIRVHKDVIKSLLLYDCFGNIG
                     QLKSDIQLMSARAFLNYKTGLKDNIEIDLSLVPDYIYIGLLKLNENRSNLNKLSELDY
                     MLFYEFNGNNLEVEYTKELSKYNIDTSNNGFKYSTEIPYLENDIRSMFINYMEKYSTK
                     LLSISSVTHSDYTSNEIFKVINPKIYYAVEHSLKEAEKISRNKYTKQTYIALSMHISA
                     LIENINQDTYNPKYNLYNDKIFLDKDLNIAKIVLSIIENDLQTSLPKDEVKFISMFLN
                     SMYLEPNNQSESIGVIILAHGNSTASSMCDVANSLLGTNHCNAIDMPLDVKVSTILDK
                     TIELVKECDEGKGVLLLTDMGSLIAFGELISEKTKIKTRSIEMVSTPIVLEAVRKSIL
                     PEMTLDELANSLSSLNPYIGRAAIDSTEINYPEKSREYVIITTCITGYGSAMKVADFI
                     NSSLKNISKYNINLVPHNIESFKMYKNKFSNTKILAVVGSVDFNVANTPFIHIEELLN
                     GYGLDMLNKIIEGTFNANSITNIKETNLSYNSLIINFMTQNLELLDSVKLFNYVNSSL
                     FTIIELIDEDLKSKFKLGYILHCSFMIERCFKNTTFPYNNITELISKNHELYSLIREA
                     FKPTEAYFNILIPDEEIAYIIDMINEYLNK"
     gene            2911..4155
                     /locus_tag="KRL67_003584"
     CDS             2911..4155
                     /locus_tag="KRL67_003584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HBH1524420.1"
                     /translation="MEDEILKGKIKQLTILALIFIFITPVFAFADTPPVPNSSRAALL
                     IDQETKRILFEKNIDEKMPLASLSKMMTFLLAIEAVDKNQVKETDMVKIDKSTASVGG
                     STCKLKDGDEISLGELMQGLMLVSGNDAAIAIAKHIGKTEKNFVNMMNKKAEEIGMLD
                     TYYFNPNGLPIYTDPEHKEPPIENMSTAHDIVTLGKYMYDHYENQVTRITTMQVYNDT
                     KKDFTHYNTNPLLVSVPGVDGIKTGYTDNAGYCLAFSMMVPKDAKNERNHRLIGVVLG
                     DGNKKNRISSSATLLKYGKDNFHSKKIAHKGDIIETPCVDGIDDFKITVKVDKDLYGV
                     VSDNENINPKVVFKNMNYPIHKGDIVGVAKYYNDSGNFVGSVDVKSESNIGCIPLKDK
                     IKIKVAKINKKLEIKNSVCFKA"
BASE COUNT         1740 a          573 c          696 g         1468 t
ORIGIN      
        1 atatagttta aacataaaga aaggaaacgg tatatgctaa gaaaagataa agttctatta
       61 tctctacata aattatgtga aaatatgact ttaaaagacc ttcaatcaga taaaacagga
      121 ttcactacaa tagaaattgc taaccattca tctcttgata gaagtaatac cagtaaagaa
      181 ttaaataacc tatataatga aaaaaaagtt ctgaaaatat ctggtaagcc aattttattt
      241 ctacatgcta gtattataga aaatttaatt ggccgtactt tgaacagtga tgaattttct
      301 ataaataatt gtaacgagct acttaaatca agtaaaaatg actcagaaga tattttttca
      361 acattacttg gacatgactc tagtcttaaa ctacctatag aaaaagctaa atctgctata
      421 ctctatcctc caaaaggtct taatacatta ctcataggac ctactggtgt tggaaaaagt
      481 acatttgccg aaacaatgta caaatatgct gtagaaagta aaatcttttc ttctgaaagc
      541 aaattcgtag tatttaactg tgctgaatat gcagaaaatg ctaacttatt attaagtaat
      601 ttgtttggtt atgtcaaagg ttcttttact ggtgctaata aggataaatt cggcatagtt
      661 gcacaggctg atggaggcat attattctta gatgaaatac acagattacc accagaaggt
      721 caagagatgc tttttctttt gatggacaaa ggcatataca gacgattagg agaatctgac
      781 attgtgcaca aagctaatgt ctttattgtg tgtgccacaa cagaagatat acagtcttct
      841 ctacttggga cttttttaag aagaatccct gttacaatta acctacctgc tttacaggat
      901 aggtccttaa aagaaagatt atctcttatt aaatacttct ttattataga atcaaaatac
      961 accaatgcaa ctataagagt tcacaaagat gtaattaaat cattactcct ttatgattgt
     1021 tttggtaata taggtcagct taaatctgac atacaactta tgagtgcaag agctttttta
     1081 aattacaaga ctggacttaa ggataatata gaaatagatt tatcccttgt tcctgattac
     1141 atctatattg gccttttaaa attgaatgaa aatcgtagta atttaaataa attatcagaa
     1201 cttgattata tgctttttta tgaatttaat ggtaataact tagaagttga atatacaaaa
     1261 gaactctcta aatacaatat agatacatca aacaatggat ttaaatactc tacagagatt
     1321 ccttatctgg aaaatgatat aaggagtatg tttataaatt acatggaaaa gtactctaca
     1381 aaattacttt cgatatcttc agtaactcat tctgattata caagtaatga aatttttaaa
     1441 gtaatcaatc caaagattta ctatgctgtt gaacattctt taaaagaggc tgaaaaaata
     1501 tcgagaaaca aatacacaaa acaaacttat attgctcttt caatgcatat aagtgctctt
     1561 atagaaaata taaatcaaga cacatataat cctaagtaca atttatataa tgataaaatt
     1621 ttcttagata aagatttaaa tatagctaaa atagttttaa gtataattga aaacgactta
     1681 caaacatctt tacctaaaga tgaagtaaaa tttatatcca tgtttctaaa tagcatgtat
     1741 cttgaaccaa acaatcaatc tgaaagtata ggagtcataa tacttgctca tggaaattca
     1801 acagcaagca gtatgtgtga tgtagcaaat tcattgcttg gtacaaacca ttgtaatgca
     1861 attgacatgc ctcttgatgt aaaggtaagt accatacttg ataaaactat agaattggta
     1921 aaagaatgtg acgaaggtaa gggtgtactt cttttaacag atatgggctc tcttatagct
     1981 tttggagaac ttatttcaga aaaaacaaaa atcaaaactc gctcaattga aatggtatct
     2041 actccaatag ttttagaagc agtaagaaaa agtatactac cagaaatgac tttggatgaa
     2101 ttagctaact ccttaagctc tttaaatcca tatattggac gagcagcaat tgattcaaca
     2161 gaaataaact atcctgaaaa gtcaagagag tatgtaataa taactacttg tataacagga
     2221 tatggttcag ctatgaaagt agcagacttt ataaattctt cacttaagaa tattagtaaa
     2281 tacaatatta atttagttcc tcataatata gaaagtttta aaatgtataa gaataaattt
     2341 agtaatacta aaattctggc agttgttgga agtgtagatt ttaatgtagc caacacccct
     2401 tttatacaca tagaagaact actaaatgga tatggcctag atatgctaaa taaaataata
     2461 gaaggtacat tcaatgccaa tagtattaca aatataaaag aaacaaatct atcatacaac
     2521 agtttaataa taaactttat gacccaaaac ttagaacttt tggactctgt taaattattc
     2581 aactatgtca attcttcctt atttacaata atagaattaa tagatgaaga cttaaagagc
     2641 aaatttaagc taggatatat actacattgc tcttttatga tagaaagatg ttttaaaaat
     2701 acaacttttc catataataa cattactgaa ttaattagta aaaatcatga actatatagt
     2761 ttaatacgtg aagcctttaa accaacagaa gcttatttta atattctgat accagacgaa
     2821 gaaattgctt atataataga catgattaat gagtatctaa ataaataaaa tttatagcac
     2881 ttattttgtg catattaatt aatatatgaa atggaggacg agattttgaa aggaaaaata
     2941 aaacagttaa caatacttgc attaattttt atatttataa ctcctgtatt tgcatttgca
     3001 gatactccac cagtaccaaa ttcttcaaga gcagcactac ttatagacca ggaaactaaa
     3061 agaatattat ttgaaaaaaa cattgatgaa aaaatgccat tagcgagttt aagtaaaatg
     3121 atgacctttt tattggcaat agaagcagtt gataaaaatc aagttaaaga gactgatatg
     3181 gtgaaaatag acaagtctac agcttcagta ggaggttcta cttgtaaact taaagatggc
     3241 gatgaaatat ctctaggaga gcttatgcaa ggtctaatgc tggtatctgg aaatgatgct
     3301 gctatagcca tagctaaaca tattggtaaa actgaaaaga attttgtaaa tatgatgaac
     3361 aagaaagcag aagaaatagg aatgcttgat acgtattatt ttaatccaaa tgggcttcct
     3421 atatatactg acccagagca taaagaacct cctattgaaa atatgtcaac tgctcacgat
     3481 atagttacac ttggaaagta tatgtacgac cattatgaaa atcaagttac tagaataacc
     3541 actatgcaag tatataatga tactaaaaag gattttacac attataacac aaatccccta
     3601 cttgtttcag tacctggtgt tgatggaata aagacaggat atacagataa tgctggatat
     3661 tgtcttgcat tttctatgat ggtacctaaa gatgctaaaa acgaaagaaa tcatagattg
     3721 ataggtgttg tacttggtga tggtaacaag aaaaatagaa tttcttcttc tgctacttta
     3781 ttgaaatatg gtaaagataa tttccattct aaaaagatag cacataaggg tgatattata
     3841 gagactcctt gtgttgatgg tattgatgat tttaagataa cagttaaggt tgataaagat
     3901 ttgtatggtg ttgtttcaga taacgagaat atcaatccta aggtagtatt taaaaatatg
     3961 aattatccta tccataaggg agatattgtt ggtgttgcaa agtattataa tgattctggt
     4021 aattttgttg gaagtgttga tgtaaaaagt gaaagtaata taggatgtat tcctttgaag
     4081 gataagatta agattaaggt tgctaagata aataaaaaac ttgagattaa aaattctgtt
     4141 tgttttaaag catagtaaag catattatta aaagggtatg ttaaatttat aaagactcca
     4201 ctttgtggag tttttttgtt ttttacaaaa aattaaaata gtggcaacaa ttgaggatgt
     4261 gagggagagt tgccactatt ttgtataggg tattatatta tgtttttaag ctagttttac
     4321 tatcttctat gtttatataa taactcttgt gtgtgaattt aattgataat taatatgaaa
     4381 attatgtgaa gttttgtaat aaaaattaaa agtgtagaac ttatttaaaa tatatattta
     4441 aataatacaa gaatatttta aagagtacta agtttta
//
LOCUS       DAEPXF010000245         4404 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain HB27
            SAMN13376389-rid12479423.denovo.253, whole genome shotgun sequence.
ACCESSION   DAEPXF010000245 DAEPXF010000000
VERSION     DAEPXF010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13376389
            Sequence Read Archive: SRR10518432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4404)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4404)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001069624.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001069624.1
            Genome Coverage       :: 315x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/15/2021 01:54:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,165
            CDSs (total)                      :: 4,076
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 89
            rRNAs                             :: 10, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 10 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 19 of 69
            Pseudo Genes (incomplete)         :: 44 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 10 of 69
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4404
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13376389-rid12479423.denovo.253"
                     /strain="HB27"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="China: Hebei"
                     /collection_date="2017"
     gene            complement(364..1839)
                     /locus_tag="KRL67_003585"
     CDS             complement(364..1839)
                     /locus_tag="KRL67_003585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-glycerol--glycerophosphate
                     glycerophosphotransferase"
                     /protein_id="HBH1524421.1"
                     /translation="MNYNKKNEVLNLIQILIEGIDYIKNNNLSESIVNSSLEAINYMK
                     KYINNSGRSNKNLLNLIDETYVKILRLSMYKNIRLVEDCEFIISNLNYLSNIIENSLN
                     KKTKIVFMPYNAKMWNSLESIWKSAVLDEQCDCYVVPIPYYKLIDTPNGITQIYTYEG
                     NDFPEYVPIIHYDDFDLSKEKPDIIYVHNQYDDCNNATMVDSNYFSYNLKQYTNMLVY
                     VAYGILGTYPVSFYLNFYELIASRNFDKVIVQSPAFEIIAECSGINKNQILTMGSPKF
                     DSLIYNLKQNNINKDYESKLKGKIIFLWTTNLMKIPNGKDGVIDEIENVFDIIENSQE
                     YGLIYRPHPLELEYVKSKVPECFNRYKTLLDSISIKNNIILDDSVSYYESFNLSHALI
                     TDRSSVLIEYIQTKKPILIYDIDMERGYYDSRIFDIFSNYIVGEEDMDLIKFMNLVKN
                     NNDYKLNQRLNSLNSVLSNTDGSCGEKIHTNVLEYVLNNHI"
     gene            complement(1877..2617)
                     /locus_tag="KRL67_003586"
     CDS             complement(1877..2617)
                     /locus_tag="KRL67_003586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HBH1524422.1"
                     /translation="MGNATRIYEKRVDIKKDTVQDFWNKRAKKHIPENPYISVKCNDQ
                     NPDYAKNLDKYEKEIIIPKLNITRNSNILDIGCGVGRLADDIVPICNYYLGTDFAEDL
                     ISIAKKRFSDNDCDFQVCDFINTLYDTKVKSKMPFDTIILAGVTMYINDDELKIALKN
                     LLGILDKKATIYISIPIGINKRLTLKEFYSKELNSEYNVIYRTIEEYEEILKPLLEEG
                     FEIVESKSFLTDINQYSDTERHYFILKR"
     gene            complement(2630..3274)
                     /locus_tag="KRL67_003587"
     CDS             complement(2630..3274)
                     /locus_tag="KRL67_003587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NTP transferase domain-containing protein"
                     /protein_id="HBH1524423.1"
                     /translation="MPTTKTIVINCAGRGSRLGLNSNKSLIDIDGKPLIIRQLECLDD
                     YDDIRIVVGFQAEKLIEVVCSYRKDIMFVFNHDYLNTKTAASLSLGKKYSRDTVISLD
                     GDILFFKEDFDNFVNTDGEIIGVCQPYTKDPVYVKTDFINDGEFATEFSRTNGDYEWT
                     GLFKIYSKNLSSGTNHVYKMISPNLPLKIAHVELREIDTPEEYNEALKWYRNKK"
     gene            complement(3247..3864)
                     /locus_tag="KRL67_003588"
     CDS             complement(3247..3864)
                     /locus_tag="KRL67_003588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IB family phosphatase"
                     /protein_id="HBH1524424.1"
                     /translation="MKGNEMENYKFVFDLDSTLTKQEILPEISKCINKYELMQNLTNE
                     TMLGNLSFEESFKKRVDLLKCIPISKVKSIVAKINLNEKIVKFIKENPDRCTVVTNNL
                     DIWICDLMKELSLENKYYSSIAHSNGDFIDKIKVIIKKEDIIKKMKGPIVAVGDGSND
                     IKMIENADIGIGFGGVRPIAPGILKVCDYAFYEEERLCQLLKQLL"
BASE COUNT         1535 a          646 c          423 g         1800 t
ORIGIN      
        1 acaaaaattt acaatataaa ataaatttat tctaagtttg tcttttacaa taatttcatt
       61 aagaccatat attctaacat tactttccaa ttatatgctt aggagtacat atctatttaa
      121 agttttccct tttattttca cttttaggga taaaaaattt gtaattttct ctacttttga
      181 taaattttat tttagactgg ttatatagga atacagtcta aaatgttgta tagagtaaca
      241 aaataaattt aaaaaaggtc aaactttaat taatttgccc tttattatct tatatttatt
      301 aaaattgata attttttaac tccttcttaa gcctaaattt atctaattat caattaattt
      361 taactaaata tggttattca aaacgtattc taatacatta gtatgtattt tttcaccaca
      421 acttccatct gtatttgata gtacactatt taaagaattt aatctttgat ttagcttata
      481 atcattatta ttttttacta agttcataaa ttttattaaa tccatatctt cttctcctac
      541 tatataattt gaaaatatat cgaatattct tgaatcataa tatccacgtt ccatatctat
      601 atcataaatt aatataggtt tcttcgtttg aatatattct attaatacag aagacctatc
      661 cgttattaaa gcatgggata agttgaatga ttcatagtaa gatacagagt catctaaaat
      721 aatattattt tttatagata ttgaatctag caaagttttg tatctattaa aacattctgg
      781 aactttagat tttacatact caagttctaa tggatgtggt ctatatataa gaccatactc
      841 ttgactattt tcaataatat caaatacatt ttcaatctca tcaatgacac catcttttcc
      901 attaggtatt ttcattagat tagttgtcca taagaaaatt attttaccct ttaatttaga
      961 ttcataatct ttatttatat tattctgttt taaattgtaa attaaagaat caaattttgg
     1021 agatcccatt gttagtattt gatttttatt tataccacta cattctgcta taatttcaaa
     1081 tgcaggagat tgcacaataa ccttatcaaa atttctagat gctatcagtt cataaaaatt
     1141 taagtaaaaa ctaacaggat aagttccaag tattccataa gcaacatata caagcatatt
     1201 agtatattgt ttcaagttat aagaaaaata attactatct accattgtag cattgttaca
     1261 atcatcatat tgattatgaa catatattat atcaggcttc tccttagata aatcaaaatc
     1321 atcataatgt attattggaa catattcagg aaaatcatta ccttcatatg tatatatttg
     1381 agttatacca ttaggcgtat caattaactt gtaataaggt atgggaacta catagcagtc
     1441 acactgctca tccaaaaccg cagatttcca gatactttct aagctattcc acattttagc
     1501 attataaggc ataaatacaa ttttagtttt tttatttaga ctattttcta ttatatttga
     1561 taaatagttt aaattactta tgataaattc acaatcttca acaagtctta tatttttata
     1621 catggaaagt cttaaaattt ttacataagt ttcatcaatt aaattcaaca ggtttttgtt
     1681 gcttcttcca gagttattta tatatttttt catataatta atagcttcaa gagatgaatt
     1741 aactattgat tcacttaaat tattattttt tatgtaatca ataccctcta taagtatttg
     1801 tataagattt aaaacttcat tttttttatt atagttcatc agattcacct taattttgat
     1861 tttatttata ttaaaactat cgctttagta taaaatagtg cctttctgta tcagagtatt
     1921 gatttatatc tgttaagaag cttttgcttt caacaatttc aaatccttcc tctaataatg
     1981 gttttaaaat ttcttcatat tcttctatag ttctatatat aacattatat tctgaattta
     2041 attctttcga gtaaaattct tttaaagtta atctcttatt tattccaata gggatagata
     2101 tatatattgt tgctttttta tctaatattc ctaataaatt tttcaatgct atttttaatt
     2161 catcatcatt tatatacatt gtgacacctg ccaatataat tgtatcaaat ggcattttgg
     2221 atttaacttt tgtatcgtat agagtattga taaaatcaca tacttgaaaa tcacagtcat
     2281 tatcagaaaa tcttttttta gcaatagata ttaagtcttc agcaaaatct gtcccaagat
     2341 aatagttaca tataggaact atatcatcag ctaacctacc taccccacaa cctatatcca
     2401 atatatttga atttctagtt atatttagtt taggaattat tatttctttt tcatatttat
     2461 ctaaattttt agcataatca ggattttggt cattacattt cactgaaata tagggattct
     2521 ctggaatatg ctttttagct cgtttattcc aaaaatcttg aacagtatct ttttttatat
     2581 ctacacgttt ttcataaatt ctcgttgcat tacccatata aaatctcctt tattttttgt
     2641 tcctatacca ttttaaagct tcattatatt cttcaggtgt atcaatttcc cttaattcaa
     2701 catgcgctat tttaagtggt aagttgggtg aaatcatttt atatacatga tttgtaccag
     2761 agcttaaatt tttagaatat attttaaaca aacctgtcca ctcataatct ccatttgtcc
     2821 ttgaaaattc tgtagcaaac tctccatcat taataaagtc tgttttaaca tagacagggt
     2881 ctttagtata gggttgacat acaccaataa tttctccatc tgtattaaca aaattatcga
     2941 aatcttcttt gaaaaaaagt atatcaccat ctagtgatat aacagtatct ctagaatatt
     3001 ttttacctaa agataagctt gcagcagtct tagtgtttaa gtaatcatga ttaaatacaa
     3061 acattatgtc tttacgataa gaacatacaa cttcaattaa tttttctgct tgaaagccta
     3121 ctacaatacg tatgtcatca taatcatcaa gacactctaa ttgtcttatt attaatggct
     3181 tcccatctat atctattaac gacttatttg aatttaatcc tagtctactt cctctacctg
     3241 cacagtttat aacaattgtt ttagtagttg gcataaacgt tcctcctcat aaaatgcata
     3301 atcacatact tttaatattc caggagctat aggtcttacc cctccaaatc caattccaat
     3361 atcagcattc tctatcattt ttatatcatt actgccatca ccaactgcaa ctataggacc
     3421 tttcattttt tttataatat cttctttttt tattataact ttaattttat caatgaaatc
     3481 accatttgaa tgagctatag atgaataata tttattttct aaagaaagct ctttcattaa
     3541 atcacaaatc caaatatcta aattatttgt cacaacagta catctatctg gattttcttt
     3601 tataaacttt acaatttttt cattaagatt aatcttagca actatacttt taactttaga
     3661 aataggaata cattttaata aatcgactct tttcttaaaa ctttcctcaa aagataaatt
     3721 tcccagcata gtttcattag taagattttg cataagttca tatttattta tacacttaga
     3781 aatctcagga agtatttctt gtttagttaa tgttgaatct aaatcaaaaa caaatttata
     3841 attttccatt tcattccctt tcattgaagc ataaaatcct atttttgagt gataagtaca
     3901 tcaaacaata ttaagttaga aatttcaata attttacaga gtttataatt ttataacata
     3961 tttttaattt ttattataaa acaatatatg gttcaatcaa aaaagctaaa aactaaatct
     4021 tatcaaaatg aagaaatctt gcgttcttaa ctaacaaaaa tactctataa atggttttta
     4081 agtattaaaa taacttgata aaaattcaat tgttacaaat actgctgttt tatgtcaagt
     4141 taattcaatt atttaaaaac actaaaatca ttgaaattac taaaaaattg tcttaatgtg
     4201 caaaatcttc gtttttagga tattgcccga tttaattata tacatattat cgtctaattt
     4261 taaaaaaaat taagagaaaa tatgtataat tttcatttat attgtttttt gttgtttatt
     4321 ttttgaacat gcttgtctat tttattatta taagcaaata tgataaaatt agtttccctt
     4381 gaatcaaatc ttctatatac taaa
//
LOCUS       DAEPXF010000246         4367 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain HB27
            SAMN13376389-rid12479423.denovo.254, whole genome shotgun sequence.
ACCESSION   DAEPXF010000246 DAEPXF010000000
VERSION     DAEPXF010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13376389
            Sequence Read Archive: SRR10518432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4367)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4367)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001069624.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001069624.1
            Genome Coverage       :: 315x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/15/2021 01:54:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,165
            CDSs (total)                      :: 4,076
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 89
            rRNAs                             :: 10, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 10 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 19 of 69
            Pseudo Genes (incomplete)         :: 44 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 10 of 69
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4367
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13376389-rid12479423.denovo.254"
                     /strain="HB27"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="China: Hebei"
                     /collection_date="2017"
     gene            138..1613
                     /locus_tag="KRL67_003589"
     CDS             138..1613
                     /locus_tag="KRL67_003589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HBH1524425.1"
                     /translation="MDSEFANAVELSYECAPLLESLKEYSEKDIACFDVPGHVKNRGV
                     AILNKYLGESLMKMDINSSPTMDNVAAPNGIIKNAQDLLAQAYMADEAFFITNGTTQA
                     IHAMILSVIKPGEKVLLPRNIHKSVINALILCGGIPIFIQPEFDEKLGISLNITLEKV
                     RTEIEKDCNIKALFFLNPTYYGVCADLERIIELCHKNNVLVLVDEAHGAHFPFHLDLP
                     PSAISLGADMVAVSIHKTGGALTQSSALLLNRDNVSFEKVLQSINMLQSTSASYLLMA
                     SIDGARVNLVENGEKQLSKALNLSRYAKSKLNKIDGIKVLSTEILKQKGVKFIDETKL
                     CINVKELNLTGFEVYDLLYKNFSIQVELGDSYNILALVSIGTNKSDIDRLVKALSIIA
                     KIYRKESTLNEFNMVQINPIIKLNPREAFYAPKESVEINSCIDRICGESIMAYPPGIP
                     IIAPGELITEEIMEYIIFLKNSNAYLTDVQDKNLDRILVIK"
     gene            1700..2113
                     /gene="speD"
                     /locus_tag="KRL67_003590"
     CDS             1700..2113
                     /gene="speD"
                     /locus_tag="KRL67_003590"
                     /EC_number="4.1.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine decarboxylase"
                     /protein_id="HBH1524426.1"
                     /translation="MKFETLGRHILVEYYNCDENVLKDPRLIENFMNDSALNAKATIV
                     DSVFHHFNPWGVSGAVIIAESHLTIHTWPEYGYAAADFFTCGDIDPWKSFELLEQLLK
                     SERSESTEIPRGLTTKIKKYSKKDLGKITHKPEAV"
     gene            2143..2994
                     /gene="speE"
                     /locus_tag="KRL67_003591"
     CDS             2143..2994
                     /gene="speE"
                     /locus_tag="KRL67_003591"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HBH1524427.1"
                     /translation="MELWYTEEWTENVRFSIKVNKHLFEGKSQFQRIDVFDSDEFGKF
                     LTIDGLMMVTYKDEFIYHEMITHVPMATNLNIKKVLVIGGGDGGTVRELSRYPQIEKI
                     DMVEIDKMVVDVSKEYMDICSCKLDDKRVSLYFEDGVNFVKCADDKSYDLIIVDSTDP
                     IGPGEGLFSTDFYKDCYRILTDDGILVNQSESPYFDFNAKEMKRANKKLKQIFPISEV
                     YQAHIPTYPSGHWLFGFASKKLNPVKNQDRNGWEKLSLKTKYYNSDIHLGSFMLPQYV
                     KEMLDEE"
     gene            2984..3862
                     /gene="speB"
                     /locus_tag="KRL67_003592"
     CDS             2984..3862
                     /gene="speB"
                     /locus_tag="KRL67_003592"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HBH1524428.1"
                     /translation="MKNNFYHMNTFMSMDKNYEESNLIVFGVGFDGTTSNRPGARFAS
                     SSMRKEFYGLETYSPFLDLDLEDYNICDYGDLEISVGSTEQVLKEIYQETYKIVRDSK
                     IPFMIGGEHLVTLPAFKAVYEKYNDIYVIHFDAHTDLREEYNNSKNSHATVIKRIWDI
                     VGDNKIFQFGIRSGTKEEFKFATEEKHTYMEIGGIDTFENIVNMLNGKNIYLTIDLDV
                     LDASVFPGTGTPEPGGVNYREFQEIFKIIKNSNINIVGCDIVELSPDYDTTGVSTVIA
                     CKILRELCLIISDKIK"
     gene            complement(4042..4242)
                     /locus_tag="KRL67_003593"
     CDS             complement(4042..4242)
                     /locus_tag="KRL67_003593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000809131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HBH1524429.1"
                     /translation="MKNGIVKWFNNEKGFGFISVEGEDDVFVHFSAIQNDGYKTLEEG
                     EKVSFDITQGNRGPQAENVNRI"
BASE COUNT         1728 a          497 c          746 g         1396 t
ORIGIN      
        1 aaaaaacact tgtaaaaatg ctgaaatatt aatataataa atatcatgag tttaataaaa
       61 gtaaaaataa aaaatagaaa taataaataa taaatattaa aaatatttga atatagagag
      121 gagatgaatt tgaaatcatg gatagcgagt ttgcaaatgc agttgaatta agctatgagt
      181 gtgctccatt attagagtcg ttaaaagaat actcagagaa agacatagct tgttttgatg
      241 tgcctggaca tgtaaaaaat agaggtgtag ctatattaaa taaatatcta ggtgaaagtc
      301 tcatgaaaat ggatataaat tcatcaccaa ctatggataa tgtagctgcc ccaaatggga
      361 ttataaaaaa tgcacaagat ttgctagcac aagcatatat ggctgatgag gcattcttta
      421 taacgaatgg cacaactcaa gcaatacatg caatgatttt gagtgttata aaaccaggtg
      481 aaaaagtatt acttccaaga aatatacata aatcagtaat aaatgcgtta atactttgtg
      541 gaggaatacc aatatttata caaccagaat ttgatgaaaa actaggtata agtttaaata
      601 ttactcttga aaaagttaga acagagatag aaaaagattg taatataaaa gcattatttt
      661 tccttaatcc aacttactat ggagtttgtg cagaccttga gcgtattata gaactatgcc
      721 ataaaaataa tgttttagtg ttagttgatg aagctcatgg tgcacatttt ccatttcatt
      781 tagatttacc tccttctgca ataagtttgg gagctgacat ggtagctgta agtatacata
      841 aaactggagg agcattgaca caatcttcag cactcttatt gaatagagat aatgtaagtt
      901 ttgaaaaggt tcttcaatca atcaatatgc tacaatcgac atcagcatct tatttactta
      961 tggcaagtat agatggtgct agagttaatc tagttgagaa tggtgaaaag caactgtcaa
     1021 aagcattaaa tctatctagg tatgcaaaat ctaaattgaa caaaattgat ggaataaaag
     1081 ttttatctac agaaatattg aaacaaaaag gtgttaaatt tatagatgaa acaaaattgt
     1141 gtataaatgt aaaagaatta aatttgactg gatttgaagt atacgattta ttatataaaa
     1201 atttttcaat tcaagtagaa ttgggagatt catataatat actggcactt gtgtccattg
     1261 gaacaaataa atcagatata gatagattag taaaagcact ttctataatt gcaaagattt
     1321 atagaaaaga atcaacatta aatgaattta atatggttca aataaatcca attattaaac
     1381 ttaatccaag agaagctttt tatgcaccaa aggaaagtgt agaaatcaat tcatgtatag
     1441 atagaatttg tggagaatca ataatggcat atccaccagg tataccgatt attgctccag
     1501 gagaattgat aacagaagaa attatggagt atattatttt cttaaaaaat agcaatgcgt
     1561 atcttacaga tgtacaagat aaaaacttag atagaatact ggtaataaaa taaattactt
     1621 tattttgcaa gggcaagtgt ttgttaagta gagtttatca aatagaactt attaaaagta
     1681 taaaggagtt ggggttgaaa tgaaatttga aacactagga agacatatac tagtagaata
     1741 ctataattgt gatgaaaacg tacttaaaga cccacgtctt atagaaaatt ttatgaatga
     1801 ttcagcatta aatgcaaaag cgacaattgt ggattctgta ttccaccatt ttaatccttg
     1861 gggggtttca ggagctgtta taattgctga atctcatttg acaatacata catggcctga
     1921 atatggttat gcagcagcag atttttttac ttgtggtgat atagacccat ggaaaagttt
     1981 tgaattatta gagcaacttt taaagtctga aagaagcgag tctacagaaa ttccaagagg
     2041 cttaactaca aaaattaaaa aatattcaaa aaaagattta ggtaagatta ctcataaacc
     2101 agaagcagta taaaacatac aagagaaagt gaggattatt atatggaact ttggtataca
     2161 gaagaatgga cagaaaatgt aagattttct ataaaagtaa ataaacatct atttgaagga
     2221 aagtctcaat ttcaaaggat agatgtgttt gatagtgatg aatttggaaa atttctaaca
     2281 atagatggat taatgatggt aacatataaa gatgaattta tataccatga gatgataact
     2341 catgttccta tggctacaaa tttaaacata aagaaagtat tagtcatagg tgggggtgat
     2401 ggaggtacag taagagaatt aagccgttat cctcaaatag aaaagattga catggttgaa
     2461 atagataaaa tggttgtaga tgtatctaag gagtatatgg atatatgctc atgtaaatta
     2521 gatgataaaa gagtaagttt atattttgaa gatggtgtta attttgttaa gtgtgcagat
     2581 gataaatcat atgacttgat aatagtagat tcaactgacc caataggacc aggcgaaggg
     2641 cttttctcaa ctgattttta taaggattgt tatagaatac tgactgatga cggaatttta
     2701 gtaaatcaaa gtgaaagtcc atattttgac tttaatgcta aagagatgaa aagagctaat
     2761 aaaaaattaa aacagatatt tccaatatca gaagtctatc aagcacatat accaacatat
     2821 ccatctggtc actggttgtt tggatttgct tcaaaaaaac ttaatccagt aaaaaatcaa
     2881 gatagaaatg gatgggagaa attaagttta aaaactaaat attataatag tgatatacat
     2941 ttaggttcgt ttatgttacc tcaatatgtg aaggagatgc tagatgaaga ataatttcta
     3001 tcatatgaac acctttatga gtatggacaa aaattatgaa gaatctaatc ttatagtatt
     3061 tggtgttgga tttgatggaa ctacttctaa cagacctggg gctagatttg caagtagctc
     3121 tatgagaaaa gaattttacg gtcttgagac atatagtcct tttttagatt tggatttaga
     3181 ggattataat atatgtgatt atggagattt agaaattagt gttggaagta cagaacaagt
     3241 cttaaaagaa atctatcaag agacatataa gattgttaga gattcaaaga taccctttat
     3301 gattggagga gagcatttag ttacattacc agcctttaaa gcagtatatg aaaagtacaa
     3361 tgatatatat gtaattcatt ttgatgccca tactgatttg agggaagaat ataataatag
     3421 taaaaattct catgcaacag taattaaaag aatatgggat attgtaggcg ataataaaat
     3481 atttcaattt ggtataaggt ctgggacaaa agaagaattt aaatttgcta cagaagaaaa
     3541 acacacatac atggaaatag gaggaataga tacatttgaa aatatagtta atatgctaaa
     3601 tggaaagaat atttatctaa ctatagattt agatgtattg gatgcatctg tatttccagg
     3661 aacaggtaca ccagaacctg gtggtgtaaa ttatagagag tttcaagaga tttttaagat
     3721 tataaaaaac tctaatataa atatagttgg ttgtgatatt gtagagttaa gccctgatta
     3781 cgatacaaca ggtgtatcga cagttatagc ttgtaaaatc ttaagagagt tatgcttaat
     3841 aatatctgat aaaattaaat agagtatttt atataaataa tatatattat aatataagaa
     3901 gttattttaa aaaagttagt ttaagtttga cgcttttcta aagtaacttc ttatttttgt
     3961 aattaaaaat aaatataata aacaacaaaa aagagccctc gatagctcct tttctaaaat
     4021 atatatattt aaattaaata attatattct atttacgttt tcagcttgag gtcctctatt
     4081 accttgagtt atatcaaagc taaccttttc gccttcttct aaagttttgt atccatcatt
     4141 ttgtatagct gagaaatgta cgaaaacatc atcttctccc tctacagata tgaacccaaa
     4201 tcctttttca ttgttaaacc attttactat tccgtttttc attataagaa aacctccgca
     4261 ttattaaaaa atacaaacta aaaaaataag tctgttctat taatataaca taaaatttaa
     4321 ataaaagcaa ataaaatttt taaaaaaatt aattactttt agaattt
//
LOCUS       DAEPXF010000247         4351 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain HB27
            SAMN13376389-rid12479423.denovo.255, whole genome shotgun sequence.
ACCESSION   DAEPXF010000247 DAEPXF010000000
VERSION     DAEPXF010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13376389
            Sequence Read Archive: SRR10518432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4351)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4351)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001069624.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001069624.1
            Genome Coverage       :: 315x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/15/2021 01:54:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,165
            CDSs (total)                      :: 4,076
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 89
            rRNAs                             :: 10, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 10 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 19 of 69
            Pseudo Genes (incomplete)         :: 44 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 10 of 69
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4351
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13376389-rid12479423.denovo.255"
                     /strain="HB27"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="China: Hebei"
                     /collection_date="2017"
     gene            82..963
                     /locus_tag="KRL67_003594"
     CDS             82..963
                     /locus_tag="KRL67_003594"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HBH1524430.1"
                     /translation="MLFKGSAVALVTPFTEDNNVNFEKLGELIEYHIENGTDALVVCG
                     TTGEATTMSESEIFAVIKYTVEKVNKRIPVIAGTGSNNTMLSVHMSQEAEKLGVDGLL
                     IITPYYNKTNEKGLKLHFETIANSVKLPIILYNVPGRTKVNIKPSVVAELAKIDNIVA
                     VKEASGDLAQVAEIAKLVPKDFTIYSGNDDTILPLLSLGGSGVISVLANICPKETHDL
                     VTKFFEGDIEGSKKLQLDMDALIAALFIEVNPVPVKTAMNILGFNVGDLRLPLAEMEE
                     TNLNVLKQELTNSGFKF"
     gene            1393..2394
                     /locus_tag="KRL67_003595"
     CDS             1393..2394
                     /locus_tag="KRL67_003595"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HBH1524431.1"
                     /translation="MKKVNVAIVGATGMVGRTFLKVLEERNFPIENLFLFSSSKSAGS
                     KVMFCGKEYIVEELKETSFTDRGIQIALFSAGGSISEKYAPIAASNGILVVDNSSQWR
                     MNPDVPLVVPEVNPEAIKNHKGIIANPNCSTIQAMVPLKALDDKYKIKRIVYSTYQAV
                     SGSGVKGVEDLEKGMNGNPTGFYPHQISYNCLPHIDSFTENGYTKEEMKMVNETMKIL
                     DNYDLKITATTVRVPVKNGHSESINVEFNNPFELDDLVKTLEEAPGVVVVDNPAKNEY
                     PTAVEFDDRDEVFVGRIRRDFSVDNGVNLWVVADNIRKGAATNAIQIAEMALSYDLV"
     gene            2511..3398
                     /locus_tag="KRL67_003596"
     CDS             2511..3398
                     /locus_tag="KRL67_003596"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HBH1524432.1"
                     /translation="MIFKGSAVALVTPFTKDNKVDFDKLGELVEYQIANGTDAIVSCG
                     TTGEANTMTDEEQLATIKYVVEKVNKRVPVIAGSGSNDTMHSVNLSQEAEKLGVDALL
                     IITPYYNKANKAGLKRHFETIANSVKLPIILYNVPGRTCVNISPSLIVELAKIDNIVA
                     VKEASGDLGQVAEIASLVPDDFAIYSGNDDTILPLLSLGGQGVISVLANVCPQETHDL
                     VAKFFEGDIEGSRKLQLGMDALIAALFIEVNPIPVKTAMNLLGFNVGDLRLPLAEMDP
                     ANLEVLKKELVNFGLKVNA"
     gene            3465..4214
                     /locus_tag="KRL67_003597"
     CDS             3465..4214
                     /locus_tag="KRL67_003597"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HBH1524433.1"
                     /translation="MLKVIATGYDGKMGKILADTIREDNELELVCVAARGLDSYEGDL
                     KIYEDMSTIVEEADVVIDFSHHSNLDNILSYVLKTKTPLVIATTGYNDDEMNKIHEAA
                     KVIPVFQSYNMSLGVNVLVKLVKDAAKLLEGFDIEIIEKHHNRKVDAPSGTAVMIANA
                     IKEVLPNVENNYGRYGRSAKRQPNEVGIHAIRGGNIVGEHDALFAGDNEVLTISHQAQ
                     SRGIFASGSIAAAKYLVKQKPGFYNMDSMLG"
BASE COUNT         1613 a          544 c          783 g         1411 t
ORIGIN      
        1 ctattaaaat taattaattt gtactataat tattattgta cttaaaaaat taaatttaga
       61 taaaaaagga gatttgatta tatgttattt aaaggttctg ctgttgcttt agttacccca
      121 tttacagagg ataataatgt caactttgaa aaattaggtg aattaatcga atatcacatt
      181 gagaatggca cagatgcact agttgtttgt ggaacaacag gtgaggcgac tactatgagt
      241 gaatctgaaa tttttgctgt aataaaatat acagttgaaa aagttaataa gaggatacct
      301 gttatagcag gtactggttc aaacaatact atgttgtctg ttcatatgag tcaagaagct
      361 gaaaaattag gggtggatgg tcttttaata ataactcctt actataataa gactaatgag
      421 aaaggtctta aacttcactt tgaaacaata gcaaatagcg ttaagttgcc aattatttta
      481 tacaacgtac caggaagaac taaagtgaat ataaagccat ctgttgtggc tgaacttgct
      541 aaaatagata atatagttgc tgtgaaggaa gcaagtggag acttagctca agttgcagaa
      601 atagctaaat tagtacctaa agactttact atatactccg gtaatgatga tactatacta
      661 ccactattat cattaggtgg aagtggtgtt atctctgtac ttgctaacat atgtcctaaa
      721 gaaacacatg atttagtaac taagttcttt gaaggtgata ttgaaggctc aaaaaaatta
      781 caacttgata tggatgcttt aattgctgcc ttatttatag aagtaaatcc agttcctgtt
      841 aaaactgcca tgaacatttt aggttttaat gttggtgatt taagacttcc tctagctgaa
      901 atggaagaaa ctaatcttaa tgtgttaaaa caagaattga caaattctgg atttaaattt
      961 tagcaatatt tatataaata agttgatttt ttcttaaata tgtattatta ttagaataat
     1021 caaattttaa tatcctatta acctagggta gaggtagctg taactaagag tatccaatag
     1081 tagctggcag gtgttaatga ttggaaaaag gatttacagc cgaagaaaat atttcttgca
     1141 ataatatttt ctgggattgc acataacatg tgtaatactg tcacttattt ttgtaggtga
     1201 tgagctacag ggtttacttt gagtgataaa ttagacgtac aaagtatacc tttgtacgtt
     1261 ttttgtatat aattaaagtt gttaatataa tattaaagtt tttagatata taaaaatatt
     1321 aacatatctt acttgtataa ctaaaatcaa ataaaacaat gttttaataa tttaaaattg
     1381 gggtgaattt atatgaaaaa agtaaatgta gcaatagttg gtgctactgg aatggtaggt
     1441 agaacatttt taaaagtatt agaagaaaga aattttccaa tagaaaatct atttttattc
     1501 tcttcttcta aatcagctgg ttctaaagta atgttttgtg gtaaagaata tatagtagaa
     1561 gaattaaagg aaacttcatt tactgataga ggcatacaaa tagcattatt ctctgctggt
     1621 ggaagtatta gtgaaaagta tgctccaata gctgcttcaa acggaatctt agtagtagat
     1681 aactctagtc aatggagaat gaatccagat gtgcctttag tagttcctga ggttaatcca
     1741 gaagccataa aaaatcataa gggtataata gctaatccta actgctctac tatacaagct
     1801 atggttcctc ttaaagctct tgatgataaa tacaaaatta agagaattgt ttactctact
     1861 taccaagctg tatctggttc tggtgttaaa ggtgttgaag atttagagaa aggaatgaac
     1921 ggtaatccta ctggattcta ccctcatcaa atctcttaca actgtcttcc tcatatagat
     1981 tcattcactg aaaatggata cactaaagaa gaaatgaaaa tggtaaatga aactatgaaa
     2041 atacttgata actacgattt aaaaataact gcaactactg taagagttcc agtaaaaaat
     2101 ggtcatagtg aatctataaa tgttgaattt aacaatccat ttgagttaga tgatttagta
     2161 aaaacacttg aagaagctcc aggtgttgta gtagttgata atcctgctaa aaacgaatat
     2221 ccaactgctg tagaatttga tgatagagat gaagtatttg ttggaagaat aagaagagat
     2281 tttagtgttg ataatggtgt aaatctttgg gttgttgctg acaatataag aaaaggtgct
     2341 gcaactaacg caattcaaat agctgaaatg gctttatcat acgatttagt ataatataca
     2401 aacttgtata aatatataat tttgtaaaat attaatagta actcaaaaac aagcattaga
     2461 agtaactgta atataaaaca caaaaataaa aaagagggga ttgattttat atgatattta
     2521 aaggttctgc tgttgctcta gttacaccat ttacaaagga taacaaagtt gattttgaca
     2581 aattaggtga attagttgag taccaaatag ctaatggtac tgatgctata gtatcttgtg
     2641 gtactactgg tgaagctaat actatgactg atgaagaaca acttgctact attaagtatg
     2701 ttgttgaaaa agttaataaa agagttcctg ttatagctgg aagtggttct aatgatacta
     2761 tgcactcagt taacttaagt caagaagctg aaaaattagg agtagatgct ctattaataa
     2821 taacacctta ttacaataaa gctaataaag ctggacttaa gcgtcatttt gaaacaatag
     2881 ctaacagtgt taaacttcca attatacttt acaatgtccc tggaagaact tgtgtaaata
     2941 taagtcctag ccttatagtt gaacttgcta aaattgataa catagttgct gtaaaagaag
     3001 ctagtggaga cctaggacag gttgcagaaa tagctagttt agttccagat gactttgcta
     3061 tctactctgg taatgatgat actatacttc ctttattatc tttaggaggt caaggagtta
     3121 tatcagtact tgctaatgtt tgccctcaag aaactcacga tttggtagct aaattctttg
     3181 aaggcgatat agaaggttct agaaaattac aacttggtat ggatgctcta attgcagctt
     3241 tatttataga agtaaatcct ataccagtta agactgcaat gaatttattg ggattcaatg
     3301 ttggagattt aagattacct ctagcagaaa tggacccagc aaatttagaa gtgttaaaga
     3361 aagaattagt taactttgga ctaaaagtta atgcatagta aagattaatg catggtaaaa
     3421 aataagtttg gtcaaaagtt agcccttaat cataggagga aattatgtta aaagttatag
     3481 caactggtta tgatggaaaa atgggtaaga ttttagccga tactataaga gaagataatg
     3541 aattagaatt ggtttgtgtt gcagctagag gtctggatag ctatgaaggc gaccttaaaa
     3601 tctatgaaga tatgagtact atagttgagg aagctgatgt agttatagat ttttctcatc
     3661 actctaattt agataatatc ttatcttacg ttttaaagac taaaacacct ttagtaattg
     3721 ctactactgg atataatgat gacgaaatga acaaaataca tgaagctgct aaagttattc
     3781 ctgtattcca atcatataat atgtctcttg gtgtaaatgt attagtaaaa ttggttaaag
     3841 atgcagctaa attactagaa ggatttgata tagaaataat agaaaaacat cacaacagaa
     3901 aagtagatgc cccaagtggc acagctgtta tgattgctaa cgctataaaa gaggttttgc
     3961 caaacgttga aaataattat ggtagatatg gccgtagtgc aaaaagacaa ccaaatgaag
     4021 ttggtattca tgctataaga ggtggaaata ttgttggcga acatgatgct ctatttgctg
     4081 gagacaatga ggttcttaca ataagccatc aagctcaatc tcgtggtatc tttgcaagtg
     4141 gttctatagc tgctgccaaa tacttagtta agcaaaaacc aggattttat aatatggaca
     4201 gcatgttggg ataatagtat aaatataata atacacaata taaattaaac atatgcaata
     4261 tagatataat aatacgcaat acaaatcaaa catatataat aaatttattt aatactatgt
     4321 ctatacaata aaaactaact aaaatataaa t
//
LOCUS       DAEPXF010000248         4346 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain HB27
            SAMN13376389-rid12479423.denovo.256, whole genome shotgun sequence.
ACCESSION   DAEPXF010000248 DAEPXF010000000
VERSION     DAEPXF010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13376389
            Sequence Read Archive: SRR10518432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4346)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4346)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001069624.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001069624.1
            Genome Coverage       :: 315x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/15/2021 01:54:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,165
            CDSs (total)                      :: 4,076
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 89
            rRNAs                             :: 10, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 10 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 19 of 69
            Pseudo Genes (incomplete)         :: 44 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 10 of 69
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4346
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13376389-rid12479423.denovo.256"
                     /strain="HB27"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="China: Hebei"
                     /collection_date="2017"
     gene            245..1057
                     /locus_tag="KRL67_003598"
     CDS             245..1057
                     /locus_tag="KRL67_003598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HBH1524434.1"
                     /translation="MYLERPELYKQSEINFWDDEYISKQLLKAHLDTNFEGASRNFNF
                     IEDSVNWIVTVANPDKYPKLLDLGCGPGLYAEKFAQKGYKVTGIDFSKRSIDYAQNRN
                     KETNLNINYLFQSYLNMSYNEEFDLATLIYCDYGALSTENRRLLMEKIYDSLRPGGKL
                     ILDVFTINKYNNFEEIKYWEINEEGGFWSNKKYMCLQDNCKYNDYNTLEQTLVITEKD
                     ENVFYVWNHYFSKESFLFEIENIGFKSVEFFSNVKGEAYSDDSMTMGLVLQK"
     gene            1397..1546
                     /locus_tag="KRL67_003599"
     CDS             1397..1546
                     /locus_tag="KRL67_003599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005360675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RtcB family protein"
                     /protein_id="HBH1524435.1"
                     /translation="MPDFYIGTGCVIGTVMTIQDKIVPNLVGVYIGCGVSYSNLGKIN
                     LDLKK"
     gene            1940..2701
                     /locus_tag="KRL67_003600"
     CDS             1940..2701
                     /locus_tag="KRL67_003600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896901.1"
                     /note="N-terminal region resembles flavodoxins. C-terminal
                     ferrodoxin region binds two 4Fe-4S clusters; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EFR1 family ferrodoxin"
                     /protein_id="HBH1524436.1"
                     /translation="MIGIYFSGTGNSRYCVEKFLQEYDITANSYSIEDNELLQHINNH
                     ENIIFSYPVQYSNVPKILKDFIINHSTLWNGKRVFIIATMALFSGDGSGVLSRILKKY
                     GAITVGGLHVKMPDSIGDEKVLKHSLEYNRKLVIKAEKKVTKAAKKLKQGSPPKEGLG
                     FLSHIVGLFGQRLYFINKTNMYTDKLKINTEECIVCGKCVNLCPMKNLVIKNCMIVAN
                     GQCTMCYRCVNNCAKKAITLLGKKVVEQSNIKKYM"
     gene            2766..3548
                     /locus_tag="KRL67_003601"
     CDS             2766..3548
                     /locus_tag="KRL67_003601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HBH1524437.1"
                     /translation="MEYYIVDSFATKLFKGNPAGVCVLDRRIPLELMQKIAEENNLPE
                     TAFVIKDKGNYELRWFTPKAEIDLCGHATLAAAYVISNFIDVNVKKIDFFTQSGKLEV
                     TRNGNLYEMIFPEIMPIEIELSPQQANLIGCVPSAVYSSRDLILLLNSEQEVINYKPN
                     YAQLRKLTDWLGIIITAQGSNTDFVSRYFCPELDSEDPVTGSSHCNLIPYWSEKLGKH
                     KMVAAQLSNRGGIIQCELLKDNTVKISGEAVLFMQGAIKIDI"
     gene            3822..4241
                     /locus_tag="KRL67_003602"
     CDS             3822..4241
                     /locus_tag="KRL67_003602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjdF family protein"
                     /protein_id="HBH1524438.1"
                     /translation="MDKISGKLTVLFEEPFWVGIFERQDGKKYEACRVVFGAEPKEVE
                     VYEFILERFFSLDFGSIKLEKNVTKDKIGYKRMQRKVKKEQEKETIGTKAQNALKLQY
                     EERKQDRKNLAKNRKEEEKERLFNLKQEKRKAKHKGH"
BASE COUNT         1757 a          409 c          735 g         1445 t
ORIGIN      
        1 ttcggtgata tttgatatca taaaatcgtt gatataagta ttgctggaaa agagtgaata
       61 aaatcaaatt taataagcaa acaaagtaag ctatagtttt attgttgtgc ttaaaaatat
      121 ttggttctat tttactctta tttaaactca tctttttgaa gttaaatcac agagtgaact
      181 ctgtgatttt tttattttaa aaataaattt taaggagtgg tatgttttgt taaacaaatt
      241 atgtatgtat ttagaaagac cagagcttta taaacaaagt gaaattaatt tttgggatga
      301 tgagtatatt tcaaagcaat tattaaaagc acacttagac actaattttg aaggagcaag
      361 tagaaatttt aactttatcg aggattctgt aaattggata gttacagttg caaatccaga
      421 taagtatcct aaattacttg atttaggttg tggcccaggt ttatatgcag agaaatttgc
      481 acaaaaaggt tataaggtaa ctggaattga tttctcaaaa cgttctattg actatgcaca
      541 aaatagaaat aaggagacta atttaaacat aaattacttg tttcaaagtt atttaaatat
      601 gagttataat gaggaatttg atttggctac tttaatttat tgtgattatg gagctttgtc
      661 aactgaaaac agaaggttgt taatggaaaa aatttatgat agtttaagac ctggaggaaa
      721 attaatatta gatgtattta caataaataa atacaataat tttgaggaaa ttaaatattg
      781 ggaaataaat gaagaaggag gattttggag taataaaaaa tatatgtgtc tacaagataa
      841 ttgtaaatac aatgattata atacattaga acaaactctt gtaataacag aaaaagatga
      901 aaatgtgttt tatgtttgga atcactattt ttcaaaagaa agtttcttat tcgaaattga
      961 aaatattgga tttaaatctg ttgagttttt tagtaatgtt aaaggtgaag catattctga
     1021 cgatagtatg actatgggat tagttttaca aaaataataa ttttttcaaa atgaataaat
     1081 aaaaacttga aacctctaat aacaaaataa cataagaaaa gcttacatat tgaaaaatgg
     1141 aaacttttat gttattttta atacattaca aatttgctta tataaaaatc aatgtttgaa
     1201 ttattatttc tatgtgaaat ttatcgagag tatattttct aaacaaacaa tgttattaaa
     1261 attaagtatg tagttaaaaa tgtatagcta aaaaggatgt tatattaatg ttagaggttc
     1321 aaggaaaata taaagatatt tgcagataat agaattatgt aattaagaat ttacaacaaa
     1381 gtctaagata agaataatgc cagatttcta tataggtact ggatgtgtaa taggtactgt
     1441 catgacaata caagataaaa ttgttccaaa tcttgttgga gtctatatag gatgtggagt
     1501 ttcatattca aacctaggca aaatcaattt agatttaaaa aaatagatga ttttataaga
     1561 taaaaaatac cttatggatt taattaatac tattgatgaa gaatctcaag cacgcaaatc
     1621 aattgatgag agtattaaaa ctagaagaaa tttagttgac ataattaaaa tattgaaacc
     1681 tgtttataat tttaaatcaa attagtaatg cttaagatta aaataatatt aaagtatcaa
     1741 tgttagaatg atacaatata aggagtacta tgaaaatagt actccttact tatacatatt
     1801 aggcaatagg aagtttttta tataggtaaa ttgttgaatt aagtttatat tatttaaata
     1861 aactacttaa tttagataat ataaaaagtc agaattttaa gaatagagct aaataatctt
     1921 agttattagg agaattacta tgataggaat atattttagt ggaacaggaa attcaagata
     1981 ctgtgtagaa aaatttttac aagaatatga tataacagca aattcttatt ctattgaaga
     2041 taatgaactt ttacagcata tcaataatca tgagaatatt atttttagtt atcctgtaca
     2101 atatagtaat gtgccaaaaa tattgaaaga ttttataata aatcattcaa ctttgtggaa
     2161 tggtaaacgt gtttttatta ttgcaactat ggctctattt agtggagatg gctctggtgt
     2221 gttatctaga atattaaaaa aatatggtgc tataactgtt ggtggattac atgtgaagat
     2281 gccagatagt ataggggatg aaaaggtatt aaaacattct ttagagtata atagaaagct
     2341 tgtaataaaa gcagaaaaga aagtaactaa ggctgctaaa aaactgaaac aggggtcacc
     2401 accaaaagaa gggttaggtt ttttgagtca tattgtaggg ttatttggac aacgattgta
     2461 ttttatcaat aaaacaaata tgtatacaga taaactaaaa attaatactg aggaatgtat
     2521 tgtatgtggt aaatgtgtta atttatgtcc aatgaagaat ctagtaatta aaaattgtat
     2581 gattgttgca aatgggcaat gtacaatgtg ttatcgttgt gtaaacaatt gtgctaaaaa
     2641 agcaattaca ttgttaggta aaaaagttgt tgaacagagt aatattaaaa aatatatgta
     2701 agaaatttaa agttttattg aatgtaaatt gatgtatatt ttatgttaga aagagaggtc
     2761 aagtaatgga atactacata gttgattcat ttgcaactaa actatttaaa ggaaatcctg
     2821 ctggagtttg tgttcttgac agaagaatac ctttagagtt aatgcagaaa atagcagaag
     2881 aaaacaattt accagaaaca gcatttgtaa ttaaagataa agggaattat gaattacgat
     2941 ggtttactcc taaagcagaa attgatttat gtggtcatgc aactttagca gcagcttatg
     3001 taatatcaaa ttttattgat gttaatgtaa aaaagataga tttctttact caaagtggaa
     3061 agcttgaagt tacacgaaat ggaaatctat atgaaatgat atttccagaa ataatgccta
     3121 ttgaaataga gttatcacca caacaagcta atttgatagg ctgtgtacca agtgctgttt
     3181 attcttcaag agatttaatc ttattgctaa acagtgaaca agaagttata aattataaac
     3241 ctaattacgc acaattgcgt aaattgacag actggttagg tataataatc acagcacaag
     3301 gaagtaatac agattttgta tcaagatatt tttgtccaga attagattca gaagaccctg
     3361 ttactggttc atcacattgt aatcttattc catactggtc tgaaaagttg ggaaaacaca
     3421 aaatggtagc tgctcaactt tctaatcgtg gtggtataat tcagtgtgaa ctgttgaagg
     3481 ataatactgt aaaaatttct ggtgaagcag tcttgtttat gcagggagca ataaagattg
     3541 atatttaaat aagtataata actaatttat gatttattat gaaaaataat taatcttctt
     3601 agagttaata ataataaagt agagttaaaa agcttaattt cacttaaatc aatatgatag
     3661 atgttgatac taagattgac aagcatgatt aaaaaatata atatttaatc tataaagata
     3721 aaaacacagt ctggttggta gtccagatgc agcaatagct gtcagtaacc tgcctccttg
     3781 gttgtccgtt ctttagaaag taatttaagg aggaactagc aatggacaaa attagtggaa
     3841 aattaacagt cttatttgaa gaaccatttt gggtaggtat atttgaaaga caagatggta
     3901 aaaaatatga agcatgtagg gtagtttttg gagcagaacc taaagaagta gaagtgtatg
     3961 aatttatact tgaaagattt ttctcattag attttggtag cattaaatta gaaaaaaatg
     4021 tgaccaaaga caaaattgga tacaaaagaa tgcagagaaa agtaaaaaag gaacaagaaa
     4081 aagaaactat agggactaag gctcaaaatg ctcttaagct tcaatatgaa gaaagaaaac
     4141 aggatagaaa aaacctagct aaaaatagaa aagaagaaga aaaagaaaga ctatttaatt
     4201 tgaaacaaga aaagagaaaa gctaaacata aaggtcatta attttaatta aatatctttt
     4261 taataattgc tagaaaaagg tgtataaaat ttttaatttt atacacctta atttattgaa
     4321 aagaataagg ttttatattt caatta
//
LOCUS       DAEPXF010000249         4261 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain HB27
            SAMN13376389-rid12479423.denovo.257, whole genome shotgun sequence.
ACCESSION   DAEPXF010000249 DAEPXF010000000
VERSION     DAEPXF010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13376389
            Sequence Read Archive: SRR10518432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4261)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4261)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001069624.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001069624.1
            Genome Coverage       :: 315x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/15/2021 01:54:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,165
            CDSs (total)                      :: 4,076
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 89
            rRNAs                             :: 10, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 10 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 19 of 69
            Pseudo Genes (incomplete)         :: 44 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 10 of 69
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4261
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13376389-rid12479423.denovo.257"
                     /strain="HB27"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="China: Hebei"
                     /collection_date="2017"
     gene            246..584
                     /locus_tag="KRL67_003603"
     CDS             246..584
                     /locus_tag="KRL67_003603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBH1524439.1"
                     /translation="MANIGKIIGDRIKVYRTKLGYSQEFLAEKAGLHPTYIGQIERAE
                     TNTSINIIMKIATALDIPLELLFANIITTEGVDNSVPLECYEMINKLTEKEQIAMLEL
                     IKCIIKYKEI"
     gene            606..923
                     /locus_tag="KRL67_003604"
     CDS             606..923
                     /locus_tag="KRL67_003604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBH1524440.1"
                     /translation="MNYIGKRLKEERKKANLTSKEFASMVGVSPWYITQIESGKKNPS
                     LKTFIKFVSVLNISADILIKDITSTGKTYLENDINEELKDLNSRELNLISQIIDIIKK
                     NKI"
     gene            1073..1750
                     /locus_tag="KRL67_003605"
     CDS             1073..1750
                     /locus_tag="KRL67_003605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH1524441.1"
                     /translation="MIVIEGSDKFKIAKEYIDVEYTLFSKVTFRYEKLKLKDNTELEK
                     IKMFKYKNGYIPNKLNLSLGYGFSSYKKQIIRETVDTLRLTEIFSSENIEDIKFIKDG
                     TKKLEISIEKVVKFKRRKKRNYVCCYCPDMYRDIKLDKESINKIYNRKIKIEREVNIF
                     EDEDVIINKKVLKFPKSWTKNMQKYWLSENKYPIHSTVIDDDRYKCCNVQYTKNRVII
                     LYYIYNH"
     gene            complement(2083..2850)
                     /locus_tag="KRL67_003606"
     CDS             complement(2083..2850)
                     /locus_tag="KRL67_003606"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HBH1524442.1"
                     /translation="MKRKVQVKNITIGEGRPKICVPIIGKNKKDIIKEAKELKDACLD
                     IIEWRVDFFENVENIKEVKEVLYELRSYIPDIPLLFTFRSVVEGGAKLISRDYYTTLN
                     KEISNTGLVDLIDVELFMGDEVIDEVVNFAHKKEVKVIISNHDFNKTPKKEEIVSRLC
                     RMQELSADLPKIAVMPQNEKDVLVLLEATNEMFKIYADRPIITMSMSGMGVISRLCGE
                     IFGSALTFGAAKSVSAPGQISFKELNSVLNLLHKSIN"
     gene            3196..3417
                     /locus_tag="KRL67_003607"
                     /pseudo
     CDS             3196..3417
                     /locus_tag="KRL67_003607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003725701.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            3657..>4261
                     /locus_tag="KRL67_003608"
     CDS             3657..>4261
                     /locus_tag="KRL67_003608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CatB-related O-acetyltransferase"
                     /protein_id="HBH1524443.1"
                     /translation="MTIPNLNKVYPRSNDYQTVYLKNVITRENIEVGDYTIYNDFYND
                     PRDFEKNNVLYHYPINNDKLIIGKFCSIACNAKFLMTSGNHSMKSNSTYTFPIFYEEW
                     KLDINHITDAWDNKGNIIIGNDVWIGYDSIIMSGIHIGDGAIIGTRAVVTNDVPPYSI
                     VGGVPAKIIKKRFNNDTISKLLEIKWWDWPYKKIQSNIKHI"
BASE COUNT         1725 a          546 c          523 g         1467 t
ORIGIN      
        1 ctataattaa catattatta attcgtttta ttattgtagt atagctatga aattatgaaa
       61 atatttctct aattaaaaat atgatttatc catatttgtt tattgtagat acagactata
      121 caaaaataaa taacacttat ttcagtataa aaacatatat agtctatatt tagactaaaa
      181 aagtataaat agtctatata aaacaactta aactatgaat ataatctaaa gtggagggat
      241 aataattggc aaatatagga aaaataatag gagatagaat aaaagtctat agaacaaaat
      301 taggatatag tcaagagttt ctagcagaaa aggcaggttt acatccaaca tatattggtc
      361 agattgaaag agctgagaca aatacatcta tcaatattat aatgaaaatt gctactgctt
      421 tagatatacc acttgaattg ttatttgcaa atattattac aacagaaggc gttgataact
      481 ctgtaccttt agaatgttat gaaatgatta ataaattaac tgaaaaagaa caaattgcaa
      541 tgctagagtt aataaagtgc atcattaaat acaaagaaat ataagtgagg gaaatagtaa
      601 caataatgaa ttatataggt aaaagactta aagaagaacg gaaaaaagca aatttaactt
      661 caaaagaatt tgcaagtatg gttggagtat cgccttggta tataactcaa atcgaaagtg
      721 gtaaaaagaa tccaagtctt aaaacattca taaaatttgt aagtgtatta aatatttctg
      781 cagatattct tataaaagat attacttcca ctggaaagac atatttagaa aatgatataa
      841 atgaagagtt aaaagactta aattcaagag aactaaatct gatatcacag attatagaca
      901 tcataaaaaa gaataaaata taattcttta tattttgtat aaaaaaaaag gttatttgaa
      961 ataaataacc tttttttgtc tttttgtgtt aacattgtct tatagtgcta atgagtacta
     1021 ttagaccatg ttctatggcc ttattttaat ataaaacgat tggagatagg ctatgattgt
     1081 gattgaaggt agcgataaat ttaagattgc aaaagaatat attgatgtag aatatactct
     1141 ttttagcaaa gtaactttta ggtatgaaaa gttgaaactt aaagataata ctgaattgga
     1201 aaaaattaag atgtttaaat ataaaaatgg ctacatccct aataaattaa acctttcttt
     1261 aggatatgga ttctcttctt ataaaaagca aataattaga gaaactgtag atactttaag
     1321 attgacagaa attttttcaa gcgagaacat agaagatatt aaatttataa aagatggtac
     1381 aaaaaaatta gaaattagca tagagaaagt tgtgaaattt aaacgtcgaa aaaaaagaaa
     1441 ttatgtttgt tgctattgcc ctgatatgta tagagacata aaactcgaca aagaatctat
     1501 caataagata tacaacagaa aaataaaaat agaaagagaa gttaatattt ttgaagatga
     1561 ggatgttata ataaacaaaa aagtattgaa gtttccaaag tcttggacaa agaatatgca
     1621 aaaatattgg ttaagtgaaa ataagtatcc catacattct actgtaattg atgatgatag
     1681 atataaatgt tgtaatgtac aatatacaaa aaatagagtg ataatattat attacatata
     1741 taaccattaa tatacataat tacaaaaaaa taatgacaaa tatacataat acaaatatat
     1801 cttctacttt atataaaaca gttattatga aaacttacta tctataaata atagaaatgc
     1861 tagtatacca tctataatct aatgtctagt aggttttaat ggcatatttt aaatatgcca
     1921 tagttgattt ataagatttt ctatatcact ttcaaaatac acactaaata gttgcttttc
     1981 agatataatt gcccaattta aattttacta tctgaaaagt tttttatatt taatttatat
     2041 cttcaaaaat taaatacctt ctaatatttc tatataatta aattaattaa tactcttatg
     2101 taataaatta agtactgaat ttaactcttt aaatgatatt tgtcctggcg ctgaaacact
     2161 ttttgcagct ccaaaagtca atgcagaacc aaatatttca ccacacaatc tacttataac
     2221 gcccattcct gacattgaca ttgttattat tggtctatct gcataaattt taaacatttc
     2281 atttgtagct tcaagtagca ctaacacatc tttttcattt tgcggcataa cagctatttt
     2341 gggtaaatct gcgctcaatt cctgcattct acataaacga gatacaatct cttctttttt
     2401 tggagtttta ttaaaatcat gattagatat aattactttc acttcttttt tatgagcaaa
     2461 attaactact tcatctataa cctcatcccc cataaataac tctacatcta ttaaatctac
     2521 taaacctgta ttagaaattt ctttattcaa agtagtataa taatctcttg atattaattt
     2581 tgctccccct tctactacac ttctaaatgt gaacaataaa gggatatcag gtatataact
     2641 tcttaattca tagagaactt ccttaacttc tttaatattt tctacattct caaaaaaatc
     2701 aactcgccac tctataatat ccaaacatgc atcttttaac tctttagcct cttttataat
     2761 atctttttta tttttcccga taattggtac acaaatttta ggtctacctt ctccaatagt
     2821 aatatttttt acttgaactt ttcttttcat attctcctcc ataatcagaa attctatagg
     2881 actttaaatt ttattttcta cacaatttaa ttatacatta taaatactat acattataaa
     2941 aatactaatt taaaagtaat ttttattcta attcttaaaa caatcccaca tattttagaa
     3001 aagatactct ataatataca aacacttatt tcttacctaa atttatttta acattctata
     3061 attttcacaa tattccttaa attttactca tataatcaat attattctgc actctaatag
     3121 aattaatcta taaatcatag tataattata cagaaataac tcgaatttca ccacttatga
     3181 agggatttaa tattaatgaa tctaaatcat ttgcattact tcagagtttt agctaaagtt
     3241 gaacactata caaaagcagc tactcaattg tctatacccc aaccaagctt aagtaatgct
     3301 atatagattc tcgaaaaaga attgagtact tttttatttg aaaaacaagg tagaaatgtt
     3361 tgcctcacca aatacggaag gtttttccta acctatgttg ataaagcatt aagttaataa
     3421 aaaaaagttg catataaaat atattaaatt gttgcaagtt ttattgtttt gatatacaat
     3481 caactaaaca tataatcttt ataaaatact atacagttaa aagtctgttt ttaaaaacaa
     3541 aatgtataaa ttataaatta aaatatagat actatcaagg agtgattctg tgaagagatg
     3601 tattgtagta gtggaatagt caaaaacata atatatatca aaggagagat ttaaatatga
     3661 ctattccaaa tttaaataag gtatatccaa gaagtaatga ttatcaaact gtatatctta
     3721 aaaatgtaat aacaagagaa aatatagaag ttggagatta cacaatatac aatgactttt
     3781 ataatgaccc aagagatttt gagaaaaaca atgtattata tcattaccct ataaacaatg
     3841 ataaattaat aattggtaag ttttgctcaa tagcatgtaa tgcaaaattt ctaatgacta
     3901 gtggaaatca ctcaatgaaa tcaaactcca catatacctt tcccatcttt tatgaagaat
     3961 ggaaactcga tatcaaccat ataacggacg cttgggacaa taaaggtaac attataattg
     4021 gaaatgatgt gtggataggt tatgattcta taattatgtc aggtatacat attggtgatg
     4081 gtgcaattat tggaacaaga gcagtagtta ctaatgacgt cccaccgtat tctattgttg
     4141 gaggagtacc tgcaaagatt ataaaaaaga gatttaataa tgatacaatt tcaaaattat
     4201 tagaaattaa atggtgggat tggccatata aaaaaattca atcaaacatt aagcatattc
     4261 a
//
LOCUS       DAEPXF010000250         4253 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain HB27
            SAMN13376389-rid12479423.denovo.258, whole genome shotgun sequence.
ACCESSION   DAEPXF010000250 DAEPXF010000000
VERSION     DAEPXF010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13376389
            Sequence Read Archive: SRR10518432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4253)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4253)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001069624.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001069624.1
            Genome Coverage       :: 315x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/15/2021 01:54:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,165
            CDSs (total)                      :: 4,076
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 89
            rRNAs                             :: 10, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 10 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 19 of 69
            Pseudo Genes (incomplete)         :: 44 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 10 of 69
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4253
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13376389-rid12479423.denovo.258"
                     /strain="HB27"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="China: Hebei"
                     /collection_date="2017"
     gene            322..2421
                     /gene="nrdE"
                     /locus_tag="KRL67_003609"
     CDS             322..2421
                     /gene="nrdE"
                     /locus_tag="KRL67_003609"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HBH1524444.1"
                     /translation="MNWIELNNQVIIKNEQGKYQLEKDKEALSSYIEEFIQPKLRKFN
                     DLEERLKYLIDEGYYSKEVMNQYSIGFIKNIYDMIDKHKFEFQSYMSANKFYQNYALK
                     SNDGKEILETYNDKVLIVALTLGNGDKDLALNLADKIVKQEFQPATPTFLNAGRKRAG
                     EMVSCFLLSVEDSTEGISYAISSSNHLSKIGGGVALNLSKLRASGESIKDIEGAAGGV
                     VGVAKMLEQSFSYFNQMGARQGSGAVYLTVFHPDFELLMDTKKINADEKIRLATLSLG
                     AIIPDKFMELAEKNEVAYAFYPHTVYKKYGVSLDEIEMDEWYDKLVNDSDIRKKEINP
                     RQMLTKIAQMQQESGYPYVVYIDTANREHTLKDVGTIKMSNLCCEIFQYQTPSEIEGY
                     GGKNEWGQDISCNLGSLNIANVMDNKTIESTVETAIRALSFVADSTDIKPVPTVSNSN
                     SKSHSIGLGAMNLHGYLVRENILYTSDDAIDFSNVFFAMVRYYSIKASMKIAIEKNQT
                     FEGFDKSEYAKGRNSKVLSKYYEQSYLPKSEKVKALFEGIYIPTKEDWTKLLDEVKEK
                     GIYNAYLMAVAPTQSISYVQNATSSIMPITEPVEVRTYGDSTTIYPMPFLTNDNMLYY
                     QSAYRMDMRKVIDLVATVQNHVDQGISTTLFITDEKTTRDIARHYIYAYKKGLKSLYY
                     TRTKMTRDTHECLVCSV"
     gene            2434..3405
                     /gene="nrdF"
                     /locus_tag="KRL67_003610"
     CDS             2434..3405
                     /gene="nrdF"
                     /locus_tag="KRL67_003610"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HBH1524445.1"
                     /translation="MTFNLHKIHNAVNWNREEDGFTQVFWEQNVKQFWLPEEISVSKD
                     IKVWNELSDKEKELYKKVLGGLTLLDTKQGNNGIPSMMNLTENLQRKAVLSFMGTMEE
                     IHAKSYSSIFMTLLSNLEIDELFEWIETEPTLQRKADLVLAQYENTTNQEGLYLSMVT
                     SVFLESFLFYSGFFYPLYLSGQGKMVASGEIISLILRDESLHGKYIGLLAQEIYDSFD
                     KIDKEILEEKMYSILYSLMENEIEYTNVIYKESGLEKEVVNFLKYNANRALENLGFEK
                     LYTVDAINPIVLNGLSTETKTHDFFSTKGNGYQKGVYEELEDEDFII"
     gene            3481..4107
                     /locus_tag="KRL67_003611"
     CDS             3481..4107
                     /locus_tag="KRL67_003611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HBH1524446.1"
                     /translation="MKYEWRKRDKELYLPKKNPVIIEVPKQKFIMLKGSGDPNSKEFT
                     EAIGVLYSLAYAIKTMPKKGIVPEGYFDYTVFPLEGIWDKGEKSKCLDVLIKEDLIYT
                     LMIRQPDFVTQDLLDKATDIVKKKKPHNLLEKVRFGSINEGLCVQMLHIGSYDNEPES
                     FDIMNEFCKKNKLRRKSYIHHEIYISDARKVSPESLKTVLRFEVEREV"
BASE COUNT         1690 a          455 c          792 g         1316 t
ORIGIN      
        1 agagataatt taaattttta aacagggcat aataagcttg tattgtttta tatttgagtt
       61 gataaaaatt ttttaaaata aaaataatgt cagttataca gtaataaaag tttagatata
      121 aaaaaatttg tgtatatact atatgtagta tataatagat aaagcactga ctatatgtag
      181 tgcttttgtt tttgaaaaat acttattgca tttctataat tatagattaa ttttgggatt
      241 tcaactgata agtaccaaaa gaaataataa gcaaattaaa tgaaattatg gcacaagatt
      301 atgttaaaag gacggtaaat aatgaattgg atagagctta acaatcaagt gataattaaa
      361 aatgaacaag gcaagtacca attggaaaag gataaggaag ccttaagtag ttacatagaa
      421 gaatttatac aaccaaagtt gagaaaattt aatgacctag aagagagatt gaagtattta
      481 atagatgaag gttattattc aaaagaagta atgaatcagt atagcatagg ttttattaaa
      541 aatatatatg atatgattga taaacataag tttgaatttc aatcatatat gagtgcaaat
      601 aagttttatc aaaattatgc attaaagagc aatgatggaa aggaaatatt agaaacatat
      661 aatgataaag tgttaattgt agctttgaca ctaggaaatg gagataaaga tttagcttta
      721 aacttggcag ataaaatagt aaaacaagaa tttcaaccag ctacacctac ttttttaaat
      781 gctggaagaa aaagggctgg agaaatggta tcatgttttt tactatctgt agaagatagt
      841 acagaaggta tatcgtatgc tatatcttca tcaaatcact tatcaaagat tggaggagga
      901 gttgcactaa atttatctaa acttagagca tctggcgaat ctatcaaaga tatagaaggt
      961 gcagctggag gtgttgttgg agtagctaaa atgttagaac aatcatttag ttactttaat
     1021 caaatgggag ctagacaagg ttctggtgcg gtgtacctta ctgtgttcca tcctgatttt
     1081 gaattattga tggatactaa aaagataaat gcagatgaaa aaataaggct tgctacatta
     1141 tcgttaggag ctataatacc agataaattt atggagttgg cagaaaaaaa tgaagtagcg
     1201 tatgcatttt atcctcatac tgtgtataaa aaatatggag tttctcttga tgagatagag
     1261 atggatgagt ggtatgataa attggttaat gatagtgata taagaaaaaa agaaataaat
     1321 cctaggcaga tgttgactaa gatagcacag atgcaacaag aaagtggtta tccatatgta
     1381 gtgtacatag atacagctaa tagagaacat acattaaaag atgttggaac gattaagatg
     1441 tctaacttat gttgtgagat atttcaatat caaacacctt cagaaataga ggggtatgga
     1501 ggaaaaaatg aatggggaca agatataagt tgtaacctag gttctttaaa tatagccaat
     1561 gtgatggata ataaaactat agaaagtaca gttgaaacag cgataagagc actgtcattt
     1621 gtagcagata gcacagatat aaaaccagtg ccaacggtaa gtaatagtaa cagcaagtct
     1681 cattctatag gattaggagc tatgaattta catggatact tagtaagaga aaatatttta
     1741 tatacttcag atgatgctat agatttttct aatgtatttt ttgcaatggt tagatactat
     1801 tcaattaaag cttctatgaa aatagctata gaaaaaaatc aaacttttga aggctttgat
     1861 aaatcagaat atgctaaggg aagaaatagt aaggttttat caaaatacta tgaacagtct
     1921 tatctaccta agtcggaaaa ggtaaaagca ttatttgaag ggatttatat accaacaaaa
     1981 gaagactgga caaaattatt ggatgaggta aaagaaaaag ggatatataa tgcatattta
     2041 atggcagtag caccaactca aagcattagt tatgtccaaa atgctacatc aagtataatg
     2101 ccaattactg agccagtaga agtaagaacg tatggagatt ctactacaat atatccaatg
     2161 ccatttttaa caaatgataa tatgttgtat tatcaatcag catacaggat ggatatgaga
     2221 aaggtcatag atttagtggc aacagttcaa aaccatgttg accaaggtat atcaacaaca
     2281 ttgtttatca cagatgaaaa gacaactaga gatatagcta gacattacat atatgcctat
     2341 aaaaaaggtt tgaaaagttt atattacaca agaacgaaga tgacaagaga cactcatgag
     2401 tgtttagtgt gttcagtata gggagagaaa tatatgacat ttaatttaca taaaatacat
     2461 aatgcagtta actggaatag agaagaagat ggatttacac aggttttttg ggaacaaaat
     2521 gtaaaacaat tttggcttcc tgaagaaata tctgtttcta aagacataaa ggtatggaat
     2581 gaactaagtg ataaagaaaa agaattatat aaaaaagtat tgggtggttt aacattattg
     2641 gatacaaaac agggaaataa tggaatacca tctatgatga atttaacaga aaacctgcaa
     2701 agaaaagctg tcttatcttt tatggggact atggaagaaa tacatgcaaa aagttattct
     2761 tctatattta tgactttact ttctaattta gaaatagatg aactatttga atggatagaa
     2821 acagaaccaa cattacaaag aaaggctgat ttagttttag ctcaatacga aaatactaca
     2881 aatcaagagg gattatacct atcaatggtt acaagtgtat ttttagagag tttcttgttt
     2941 tattcaggat tcttttatcc attgtatcta tctggtcaag gaaagatggt agctagtgga
     3001 gaaataatat cgcttattct tagagatgag tctctacatg gtaagtacat aggattgctt
     3061 gcacaagaaa tatatgattc atttgataaa attgataagg agatattaga agagaagatg
     3121 tattctatac tttatagtct tatggagaat gagatagagt atacaaatgt tatatacaaa
     3181 gaaagtgggc ttgaaaaaga ggttgtgaac tttctaaagt acaatgcgaa tagagcttta
     3241 gaaaatctag gatttgagaa gctatatact gtagatgcta taaatccaat tgtattaaat
     3301 ggtcttagca ctgaaactaa aacacatgat ttcttttcaa ctaagggtaa tggataccaa
     3361 aaaggagtgt atgaggaatt agaggatgaa gattttataa tataatttat aattatgaca
     3421 cactgttgtc acttttagcg tgataatatc aatgtataaa atatatacgg aggtattttt
     3481 atgaaatatg aatggagaaa aagagataaa gagctatatt tacctaaaaa gaatcctgta
     3541 ataattgaag taccaaaaca gaaatttatc atgttaaaag ggagtggaga cccaaattct
     3601 aaagaattta ctgaagcaat aggagtactt tattctttgg catatgcaat aaaaaccatg
     3661 cctaaaaaag gcattgtacc agaagggtat tttgactata cggtttttcc tttagaaggt
     3721 atatgggata agggagaaaa aagcaaatgt ttagatgtac ttattaaaga agatttaatt
     3781 tatactctaa tgatacgtca gccagatttt gttacacaag acctgttgga taaagctact
     3841 gatatagtaa agaagaagaa acctcataat cttcttgaaa aagttagatt tgggtctatt
     3901 aatgaaggtt tatgtgttca aatgcttcat ataggttcat atgataatga accagaaagc
     3961 tttgatatta tgaacgaatt ttgtaaaaaa aataaactta gaagaaaatc ttacatacat
     4021 catgaaattt atataagcga tgctagaaaa gtatcgcctg aatcattgaa gacggtttta
     4081 cgttttgaag tagaaaggga agtataaaga ttttgaataa tttataagag ttataaaaag
     4141 tgaagtctgt ttagacttca ttttttgatg cagttttaaa attaaatttg atgtttcata
     4201 acatttttaa aactttctac ttttattagt gcaaataact gttactatta aat
//
LOCUS       DAEPXF010000251         4244 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain HB27
            SAMN13376389-rid12479423.denovo.259, whole genome shotgun sequence.
ACCESSION   DAEPXF010000251 DAEPXF010000000
VERSION     DAEPXF010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13376389
            Sequence Read Archive: SRR10518432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4244)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4244)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001069624.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001069624.1
            Genome Coverage       :: 315x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/15/2021 01:54:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,165
            CDSs (total)                      :: 4,076
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 89
            rRNAs                             :: 10, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 10 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 19 of 69
            Pseudo Genes (incomplete)         :: 44 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 10 of 69
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4244
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13376389-rid12479423.denovo.259"
                     /strain="HB27"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="China: Hebei"
                     /collection_date="2017"
     gene            295..2565
                     /locus_tag="KRL67_003612"
     CDS             295..2565
                     /locus_tag="KRL67_003612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HBH1524447.1"
                     /translation="MDIIKKLSHGCTLDCHDCCKFNVYTKGNNVVKIEGDKNHPYTKG
                     FICKKGMAHLDRLNHKDRIKTPMLKVDGVWKEISFDKAIEIMAEKLTYYKEKYTSKSV
                     MHYDQYGSGSVLKYIGDIFFNFYGGVSRQKGGPCWSAGMHAQKYDFGIARSHAIEDML
                     NSKSIFVWGKNPAYTTIHTMQIIKKAKEKGIKIVVIDPIYTKTAQIADKYVQVNPGTD
                     GALAIAMAKIIVEDKLYDEEYINSYVIGFEEYKKYLYSLELSFLIDECGVKENDIREL
                     VDLYTNKYSSINVGYGLQKYKNGGNTIRAIDALSAITGQIGFSGGGVNYANKVYPSVI
                     NSDPYNSQSYGEDREFYVSNISEFIEESLKNTSNKVNYASNELDTTSNKVNYVLDELD
                     ITSNKVNYVSDELDITSNKVNHVSNELGMASNKINYVSDELDITSNKTDYISNELYNL
                     SNKSIKDNIPIKMVVITKSNMLNQLPDLVELERVFSKIEFKVCFDMFMTDTATLCDLF
                     IPCTNTLESEDIIYSSMTNPYITYNERAVKPVHKLMDEYYFFMELAKKMGLNDYPFVE
                     KRTYLEKVIEPLKRFDENLDIEKLKNNYFTIHSPVAWEDKKFETPSGKYELYSERIKS
                     LGISPTPVYISNRYREIEDKNISFRLLTNHHADTLFSQHFMDKKSIAQAYINQSMAKK
                     VGIKDKDIVTLRSKKAKINVQINIDDGVGNHIVKMYVGWWKKHGNPNSLTDTGISDFG
                     GQVTYNESMVEIIRQN"
     gene            2652..2987
                     /gene="spoIIAA"
                     /locus_tag="KRL67_003613"
     CDS             2652..2987
                     /gene="spoIIAA"
                     /locus_tag="KRL67_003613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma F factor antagonist"
                     /protein_id="HBH1524448.1"
                     /translation="MVNYSLEHKNLYIEFMCSELDHHVANEIREEIDNLLSVNQVKNV
                     VFNFENINFMDSSGIGVIIGRYKKISNEGGRVSVINISSRVKKIFDLSGLNKIIGIYD
                     TYEEALSSL"
     gene            3001..3429
                     /locus_tag="KRL67_003614"
     CDS             3001..3429
                     /locus_tag="KRL67_003614"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma F factor"
                     /protein_id="HBH1524449.1"
                     /translation="MNNIMEVKFSAISENESLARVIVASFAAKLDPTVDELVDIKTAV
                     SEAVTNAIIHGYEEDSSKFVFLRCEIEGNTIKIIVEDEGYGIENVEKAMEPLYTSKPE
                     LDRSGMGFTVMKSFMDDVEVSSVKDNGTRIEMTKKINVPK"
     gene            3452..4216
                     /gene="sigF"
                     /locus_tag="KRL67_003615"
     CDS             3452..4216
                     /gene="sigF"
                     /locus_tag="KRL67_003615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sporulation sigma factor SigF"
                     /protein_id="HBH1524450.1"
                     /translation="MEVTVAREEKKPLLSHEETLELIEKVQNGDEEAKEILISSNLGL
                     VRSVVSRFLNIGYDREDLFQLGSIGLIKSIYKFDPKFNVKFSTYAVPMILGEIKRYLR
                     DDGMIKVSRSLKQIAVKAKMESEALTKKLGREPSIEELAKAIDVEKEDLVMAMEANFN
                     VEYLQGVIHEEEGSPICLIDKISMKGESEEEKVVDNILLKDILGRLDKRERQIIVLRY
                     FEDMTQSEIGEMLNISQVQVSRIEKKVLSKLKEYIS"
BASE COUNT         1727 a          417 c          802 g         1298 t
ORIGIN      
        1 aaataatata ttggatattt aaaaagccta atgtgttttc aaaacagatg tgtagaatct
       61 gtaatagtta acaacataag gcttttattg tttttatata agaaatctga ataacttact
      121 gttaaatctt taaactactc caccttactt atgttaagga tggagtattc tacttatttt
      181 tgataaaata tatctatgtt ttatatactg tagttttgaa catagaaagt ttaataaatt
      241 atagtgtata tgttaatttt taataaggta agaaagtaga gagaaggata ttttatggat
      301 ataataaaaa aattaagtca tgggtgtaca ttagattgtc atgattgttg taaattcaat
      361 gtttatacta aaggaaataa tgtagtaaaa attgaaggag ataaaaatca tccttacaca
      421 aaaggtttta tatgcaaaaa agggatggct catttagata gattaaatca taaagataga
      481 ataaaaactc ctatgcttaa agttgatgga gtatggaaag agatttcttt tgataaggct
      541 atagaaataa tggcagaaaa gcttacatac tataaagaaa agtatacatc aaagtctgtc
      601 atgcattatg accaatatgg aagtgggtct gtgctaaaat acataggaga tatatttttt
      661 aatttttatg gaggtgttag cagacagaaa ggaggtccct gttggagtgc agggatgcat
      721 gctcaaaaat atgattttgg aatagctagg agtcatgcga tagaagacat gttaaatagt
      781 aaaagtatat ttgtgtgggg aaaaaatcca gcatatacta caattcatac tatgcaaata
      841 attaaaaaag ctaaagaaaa aggtataaag atagtagtga ttgaccccat atatacaaaa
      901 acagctcaaa tcgcagataa gtatgtacag gtaaatcctg gaacagatgg agctttagct
      961 atagctatgg caaaaattat agtagaagac aaattatatg atgaagaata tataaactca
     1021 tatgttattg gttttgaaga atataaaaaa tatttatatt cgttggaatt aagtttttta
     1081 attgatgaat gtggtgttaa agagaatgat ataagagagt tagtggattt atatactaac
     1141 aaatattcaa gtataaatgt tggatatggg cttcaaaaat ataaaaatgg tggaaacaca
     1201 ataagagcta tagatgcttt aagtgctata actggtcaaa taggatttag tggtggaggg
     1261 gtaaactatg ctaataaagt ttatccaagt gttataaatt ctgaccctta caatagccaa
     1321 tcatatggtg aagataggga gttttatgta agtaatatca gtgaatttat agaagaatct
     1381 ttaaaaaata catcaaataa agtaaattat gcatcaaatg aacttgatac aacatcaaat
     1441 aaagtaaatt acgtattaga tgaacttgat ataacatcaa ataaagtaaa ttatgtatca
     1501 gatgaacttg atataacatc aaataaagta aatcatgtat caaatgaact tggtatggcg
     1561 tcaaataaaa taaattatgt atcagatgaa cttgatataa catcaaataa aacagattat
     1621 atatcaaatg aattgtataa tttatctaat aagtcaatca aagataatat tcctataaag
     1681 atggttgtga taactaaaag taatatgctg aatcaactgc cagatttagt agaattggag
     1741 agggtatttt cgaaaattga atttaaagta tgttttgata tgtttatgac tgatacagct
     1801 actctatgtg acctgtttat accttgtaca aatacactag aaagtgaaga tataatttat
     1861 agttcaatga ccaatccata tataacctat aatgaaagag ctgtaaagcc agtacataaa
     1921 ttgatggatg aatattactt ctttatggag ctagctaaaa agatggggct aaatgattat
     1981 ccatttgtgg aaaagagaac ttatttagaa aaagtgattg aaccacttaa gcgttttgac
     2041 gaaaatctgg atatagaaaa acttaaaaac aattacttta ctatacatag ccctgttgct
     2101 tgggaagaca agaaatttga gactccatct ggaaagtacg agctttattc agaacgtata
     2161 aaaagtttag gaataagccc aacaccagtt tatataagca atagatatag agaaatagaa
     2221 gataaaaata tttcttttag attattaact aatcatcatg cagatacttt atttagtcag
     2281 cattttatgg ataaaaaatc tatagctcaa gcatatataa atcaaagcat ggctaaaaaa
     2341 gtaggtatta aggataaaga tattgtgaca ttaaggtcaa aaaaagcaaa aataaatgta
     2401 cagataaata tagatgatgg agtaggtaat catatagtaa aaatgtatgt tggttggtgg
     2461 aagaaacatg gaaatcctaa tagtttaaca gacactggta tatcagattt tggtggtcag
     2521 gtaacatata atgaaagtat ggtagaaata atacgacaaa actagttaaa tttcattgaa
     2581 ggaataaaaa tataattata gaattgatta aaagttacta tttttacaaa actattgaag
     2641 gagggatttt gatggtaaat tattccttag aacataagaa tctatacata gaatttatgt
     2701 gctcagaact agaccaccat gtagccaatg aaataaggga agaaatagat aacttattaa
     2761 gtgtaaatca agtaaaaaat gttgtattta attttgaaaa tattaatttt atggattctt
     2821 caggtatagg tgtcatcata ggtagatata aaaagatttc taatgaaggg ggacgagttt
     2881 cagtaattaa tataagttca cgagttaaga agatttttga tttatctggg ttaaataaaa
     2941 ttattggcat ttatgatacg tatgaagaag ctctaagttc tttgtaggga ggagtatgtg
     3001 atgaataata ttatggaagt taaattttcg gcaatatctg aaaatgaaag tttagcaaga
     3061 gttatagtag catcctttgc agctaagtta gacccaacag tagatgagtt agtagatata
     3121 aaaactgctg tctctgaggc tgttacgaat gctataatac atggatatga agaagacagc
     3181 tctaagtttg ttttcttaag atgtgagata gaaggaaata ctataaagat tatagttgaa
     3241 gatgaagggt atggaataga aaatgtggag aaggcaatgg aaccacttta tacatcaaaa
     3301 ccagaacttg atagatcagg aatgggattc acagtaatga aaagctttat ggacgatgta
     3361 gaggtaagtt ctgtaaaaga taatgggact agaattgaaa tgactaaaaa aataaatgtg
     3421 cctaaataaa gtccaaaagt gaggttttat tatggaagta actgttgcca gagaagaaaa
     3481 aaaacctctt cttagtcatg aagagactct agaacttatt gaaaaagttc aaaatggaga
     3541 tgaagaggca aaagaaatat taatatcaag caatttaggt ctagttagga gcgttgtatc
     3601 tagattttta aatataggat atgatagaga agatttattt cagttaggtt ctataggctt
     3661 aataaaatca atatataaat ttgacccaaa atttaatgta aaattctcta cttatgcagt
     3721 accaatgatt ttaggtgaaa taaagagata cttaagagat gatggtatga taaaagtatc
     3781 aaggtcgtta aagcaaatag ctgtaaaggc aaagatggaa agtgaagctt taactaaaaa
     3841 actaggtaga gagccgagta tagaagaatt agctaaagca atagatgtag aaaaagaaga
     3901 tttagtaatg gctatggaag ctaatttcaa tgttgagtat ttgcaaggag ttattcatga
     3961 agaggaaggt tctccaatat gtcttattga taaaataagc atgaaaggag aaagtgaaga
     4021 agaaaaagta gtggataaca tattgttaaa agacatactt ggaagattgg ataagagaga
     4081 gcgtcaaata atagttctca gatactttga agatatgact caaagtgaaa taggagagat
     4141 gctcaatata tcacaagttc aagtatctag gatagagaag aaagtattgt ctaagttaaa
     4201 agaatatata tcgtgataat acttgtgata tgtagaataa caaa
//
LOCUS       DAEPXF010000252         4214 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain HB27
            SAMN13376389-rid12479423.denovo.260, whole genome shotgun sequence.
ACCESSION   DAEPXF010000252 DAEPXF010000000
VERSION     DAEPXF010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13376389
            Sequence Read Archive: SRR10518432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4214)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4214)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001069624.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001069624.1
            Genome Coverage       :: 315x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/15/2021 01:54:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,165
            CDSs (total)                      :: 4,076
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 89
            rRNAs                             :: 10, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 10 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 19 of 69
            Pseudo Genes (incomplete)         :: 44 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 10 of 69
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4214
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13376389-rid12479423.denovo.260"
                     /strain="HB27"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="China: Hebei"
                     /collection_date="2017"
     gene            88..693
                     /locus_tag="KRL67_003616"
     CDS             88..693
                     /locus_tag="KRL67_003616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HBH1524451.1"
                     /translation="MKIKENVLMLDSTRGSNCFIVFDKEIILIDTGLPFMGKNIIKEL
                     KTLGIKLTDIKHILLTHHDVDHISNIKLLKEKTGAKVWAHVEDIPFIIGEKDRPGFKK
                     FIGKAISRRLIKDVEPYGENMQIGNIKIIHTPGHTPGHVCMIFEDVLFAGDLVKNKNG
                     NIVPYPNPWNWDYKKMMKSIRELDNLKYEWICMSHGTPCIK"
     gene            794..1516
                     /locus_tag="KRL67_003617"
     CDS             794..1516
                     /locus_tag="KRL67_003617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBH1524452.1"
                     /translation="MIRTLILYYLNIKPTHGYEIQKFLQVSGADRWTKIQSGSIYYAL
                     AKLEKDGGVRVLREEKTGARIRKIYEITQSGKLELREEIQKELQMPIVPTGSNKFLLH
                     NILDVLPKDTLQKNLEKHIKYLIEQKKYWENWKEIKKIDKKSLATEKIAFDMTIDNLN
                     YQILWHEEILNNIDKYISVGCETQNIIKSIDFSNIEEDFLFKSDTTNELLEVQRLRDE
                     IINNPDKAIENIDKIILKLQNK"
     gene            1621..2379
                     /locus_tag="KRL67_003618"
     CDS             1621..2379
                     /locus_tag="KRL67_003618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBH1524453.1"
                     /translation="MSNVLLEIKNLSKKYNNKEVVSRESFKLLEGDILGFIGPNGAGK
                     STSINMLTTIVSPDSGSIYYKGKDIKNQQNIFKSSLGVVPQELAIYEDLSAYDNVKFF
                     CSLYGFKGNELKRRTKDALEFVGLWERNKEFPLKFSGGMKRRLNIACAIAHSPKILIM
                     DEPTVGIDPQSRNKILKAIKDINANGTTVIYTSHYMEEIETLCNRIVLIDKGVIIEDL
                     DKSLYKNKYLEFGCKTLEDIFLYLTGTDLRDLEE"
     gene            2384..3124
                     /locus_tag="KRL67_003619"
     CDS             2384..3124
                     /locus_tag="KRL67_003619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBH1524454.1"
                     /translation="MRYFLPLFKLGIKRRSKDFFILFYNIVFPVIIIMLLGYLTSKSY
                     GEEFTSYNYYTIVTIPFCVLMGIISVSYVAQDEKISNTSYRYMISPISIVDLVISKLF
                     SCTAVLSICNLITLAVLRGIFHMSFGGKFLVVIFFLMCESIAVVSMGLFLGLVCKNLD
                     MLRNIINIPIVIFGFLGGVFFPVSSLNPVISLIINISPLTWINRGIVACIYDNNLQLL
                     FNISCIFVAVSIFITILTIKFFKKEVFI"
     gene            3124..4176
                     /locus_tag="KRL67_003620"
     CDS             3124..4176
                     /locus_tag="KRL67_003620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBH1524455.1"
                     /translation="MNVINVFNSNLKRNINKRLVFLVTLLFPIIIVILGVLANYISKP
                     SFNIGILDEYKNERVLKESEKRTYENTIMALENTQGINTEIADFKTIKTDIITGKYSA
                     VICFNKNGFKLYSIKDKNTNDTLKYLIETYKKDPVPVDISKLQENTLGVLQRIIAFIV
                     LFLMITSTVTASMIIKDKNSGTFTRVLYSPQNIRGYVLGNMLYNFTITYFQYIVSISI
                     IKLFNIDIGMDYIDLILMGVWISALATSFGTFVSSLFNKEMHANLFSTCISLILSLIG
                     GSFISVEKMPTMLQKISIISPTQWFISFVTYIEERDGSFFNMNYICILTSIIIILALL
                     AMKFIQRSKYSSNRNY"
BASE COUNT         1628 a          420 c          694 g         1472 t
ORIGIN      
        1 aaataaaatt aatatcaagt attataacta tttataactt tattaattaa ttatataggt
       61 aaaataccaa ataaggggaa tttatttatg aagataaaag aaaatgtatt aatgttagat
      121 agtactcgtg gttcaaattg ttttattgtg ttcgataaag aaattattct tatagacaca
      181 ggtcttcctt ttatggggaa aaatataatt aaagaattaa aaacacttgg cattaaactt
      241 acagatatta agcatatttt attaactcat catgatgttg accatatatc taatattaaa
      301 ttattgaaag agaaaacagg agcaaaggta tgggctcatg tagaggatat accttttatt
      361 ataggagaga aggatagacc tgggtttaag aagtttattg gaaaggcaat ttccagaagg
      421 ctcattaagg atgttgaacc ttatggagag aatatgcaga ttgggaatat aaaaattatc
      481 catactcctg gtcatactcc agggcatgtc tgcatgattt ttgaagatgt attatttgca
      541 ggtgatttag tcaaaaataa gaatggaaat atagttcctt accctaatcc atggaattgg
      601 gattataaaa agatgatgaa atctataaga gaattggata atttaaaata tgaatggata
      661 tgtatgtctc atggaactcc ttgtattaaa taataaaaat aataagttgt aatatatcct
      721 atgttatcta ttgactatat ccaatattgg tgataatata atatccaaga tagaatatag
      781 gtggtgaatt tttatgatac gcactttaat tttatattat ctaaatatta agccaacaca
      841 tgggtacgag attcaaaagt ttttacaagt atctggtgca gatagatgga ctaaaataca
      901 atctgggtca atatattatg cattggcaaa attagaaaaa gatggaggag ttagagtttt
      961 aagagaagaa aaaacaggag ctaggattcg taaaatctat gagattactc aaagtggtaa
     1021 attagaatta agggaagaaa tacaaaaaga gcttcagatg ccaatagttc caaccggttc
     1081 aaataaattc ctattacata atattttgga tgtattacct aaggatactt tgcaaaaaaa
     1141 tcttgaaaaa catataaaat atcttataga gcagaagaag tattgggaaa attggaaaga
     1201 gattaaaaaa attgataaaa agagtcttgc aacagaaaaa attgcatttg atatgactat
     1261 agataatctg aattatcaaa ttttgtggca tgaagaaata ttaaataata tagataagta
     1321 tatatctgtt ggttgcgaga cacagaatat aattaaatct atagactttt caaatataga
     1381 agaagatttt ttatttaaat ctgatacgac aaatgagtta ttagaagttc agagacttag
     1441 agatgaaata attaataacc cagataaagc tatagaaaat atagataaga taatattgaa
     1501 attacaaaat aagtaataaa ttttattaca ataacacgag taaaatactc gtgttttaaa
     1561 aaaactatat atacaacatt ggatatataa tgttggttaa atattaggag ggttttataa
     1621 atgagtaatg tattgttaga gattaaaaat ttatctaaga agtataacaa taaggaagtt
     1681 gtatcaagag aaagttttaa gttattggaa ggagatattt taggttttat tggaccaaat
     1741 ggagcaggga aaagcacatc tatcaatatg cttactacga ttgtgtctcc agatagtgga
     1801 agcatatact ataaaggaaa agatattaag aatcaacaaa atatttttaa aagctcactt
     1861 ggagttgtac cacaagaatt agcaatatat gaagatttaa gtgcgtatga taatgttaaa
     1921 tttttttgct cattatatgg atttaaaggg aatgaactta aaaggagaac taaagatgct
     1981 ttggaatttg ttggtctttg ggagaggaat aaggagtttc cattaaaatt ttctggtgga
     2041 atgaaacgta gactaaacat agcttgtgca attgcgcata gtccaaagat tctcatcatg
     2101 gatgagccaa cagtaggtat cgaccctcaa tcaagaaata aaattttgaa ggcaattaaa
     2161 gacattaatg ctaatggtac aacagttata tatactagtc actatatgga agaaattgag
     2221 accttatgta atagaattgt tttaatagat aaaggagtaa ttatagaaga tttagacaag
     2281 agtttatata aaaataaata tttagaattt ggttgtaaga cattagaaga tatttttcta
     2341 tatcttacag gtacagattt gagagatttg gaggaataaa tagatgagat attttttacc
     2401 attgtttaaa ttaggaataa aacgaagaag taaagatttt tttattctgt tttacaatat
     2461 tgtttttcca gtaataatca taatgctatt aggatatttg acatcaaaat cctatggtga
     2521 ggaatttaca tcatataatt attatactat tgttacaata ccattttgtg ttcttatggg
     2581 aattattagt gtttcatatg tggcacaaga tgaaaaaatt tctaacacat catatcgtta
     2641 tatgatttct cctatttcaa tagtagattt agttatttca aaactttttt cttgtacagc
     2701 agtactttca atatgcaatt tgataacttt agcggtgtta agaggaattt ttcatatgag
     2761 ttttggtggg aaatttttag ttgttatttt ctttttaatg tgtgaatcta ttgcagtagt
     2821 atctatgggt ttgtttttgg gtcttgtttg taaaaattta gatatgttac gtaatatcat
     2881 taatatccca atagttattt ttggtttctt aggaggagtg ttctttccag ttagctcatt
     2941 gaatccagtt atttcattaa tcattaatat atcaccattg acatggatta atagaggtat
     3001 agttgcttgt atctatgata ataatttaca actccttttt aatatatcat gcatatttgt
     3061 agctgtaagc atatttatta caatacttac aatcaaattt tttaaaaagg aggtctttat
     3121 atagtgaatg taataaatgt ttttaatagt aacttaaaaa gaaatataaa taagcgatta
     3181 gtgtttttag ttacactttt atttcctata attatagtaa ttttaggagt attggcaaat
     3241 tacataagta aaccatcttt taatattggt attttagatg agtacaaaaa cgaacgagtt
     3301 ctaaaggaat ctgaaaaaag gacctatgaa aatacaataa tggctttaga aaatactcaa
     3361 gggattaata cagagattgc agattttaaa acaattaaaa ctgatattat aacaggcaag
     3421 tattcagctg tcatatgttt taataagaat ggttttaagt tatattctat aaaagataaa
     3481 aatacgaatg atacattaaa gtatttaata gaaacatata aaaaagaccc agttcctgta
     3541 gatataagca aattacaaga aaatacttta ggagttttac aaagaataat tgcatttatc
     3601 gtattatttt taatgataac atctactgta acagcatcaa tgattattaa agataaaaac
     3661 tcaggtacat ttacaagagt gctgtattct ccacaaaata taagaggata tgtgctaggt
     3721 aatatgttat acaactttac aataacgtat tttcagtaca tagtttcaat atctataatt
     3781 aagttattta atattgatat tggtatggat tatatagatt taattttaat gggagtatgg
     3841 atatcggctt tggcaacttc ttttggaaca tttgtatctt ctttattcaa taaagaaatg
     3901 catgcaaatt tgttttctac atgtatatcg cttatattat cacttattgg tggttcattt
     3961 atttcagttg aaaaaatgcc aacaatgtta caaaaaatta gtattataag tccaacacaa
     4021 tggtttatta gttttgtgac ttatatagaa gaaagagatg gaagcttttt taatatgaat
     4081 tatatttgta ttttgacatc tataataata attttagctt tgttagctat gaaatttatt
     4141 caaagaagta aatattcaag taataggaat tattaaataa gatagaaaat gaatagatgt
     4201 aattaaaaca aatc
//
LOCUS       DAEPXF010000253         4212 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain HB27
            SAMN13376389-rid12479423.denovo.261, whole genome shotgun sequence.
ACCESSION   DAEPXF010000253 DAEPXF010000000
VERSION     DAEPXF010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13376389
            Sequence Read Archive: SRR10518432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4212)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4212)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001069624.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001069624.1
            Genome Coverage       :: 315x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/15/2021 01:54:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,165
            CDSs (total)                      :: 4,076
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 89
            rRNAs                             :: 10, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 10 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 19 of 69
            Pseudo Genes (incomplete)         :: 44 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 10 of 69
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4212
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13376389-rid12479423.denovo.261"
                     /strain="HB27"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="China: Hebei"
                     /collection_date="2017"
     gene            149..2212
                     /locus_tag="KRL67_003621"
     CDS             149..2212
                     /locus_tag="KRL67_003621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF262 domain-containing protein"
                     /protein_id="HBH1524456.1"
                     /translation="MKAGEIEFLSYLEGSNKSFVVPVYQRNYNWKKEQCKRLFDDLED
                     IIDSGFRTHFLGSVVSIYSMGKEYLIIDGQQRVITVSILLIAMCQIIKEFGESKGIIT
                     TEEQIKECYLINKHSRDKDGKIKLKPIAEDRKAYYELFEEEMNTDRNSNIVSNYLYFY
                     NRIKDDNISIDDLFDAIQKLTIVEIELKNGEDDPQLIFESLNSTGLDLSEADRVRNFV
                     LMGRSSKLQEEFYNNYWYKIEKNTSSNVSSFIRDYLTIKERSIPNKNKVYFSFKRYVS
                     DNGIDIEILLKDLLKFSNYYREISYSCTDDNDVNKVLKRINKLEIFVSYPFILELFDD
                     YNEGIINKKVLVESLNLVECFILRRIVCDVPPNALNKVFMNLGREIKKFSDYKENYSN
                     ILRYIFINKKSSQRFPTDSEFSNAFETKDIYNTKSKNKLYILERLENYNNKEIVDLEN
                     LINNNMLNIEHIMPQTLTNKWKESLGDNYKEIHDKYLHTIGNLTLTGYNSKLSNKTFE
                     EKKEMEDGFKDSRLYLNKYISSIDKWNEEEIKNRTKILLDRAVEIWKYPSVTYEPPSN
                     SENIFTLEDDDVNFTNESIVSFSILGESYKAKNWTEFYEQVALTLYDLDSVRFNSIVD
                     KTYKRDFIDNIKSRDVYKLRKGIKISENIYLETNLNTEAKLNIIRFLLNEFDIELNEV
                     IFSIK"
     gene            complement(2429..2995)
                     /locus_tag="KRL67_003622"
     CDS             complement(2429..2995)
                     /locus_tag="KRL67_003622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HBH1524457.1"
                     /translation="MDTKKVTFSSMCIVINIVLGMVISMINIPLLFLDTVGTVLGAVV
                     LGPFWGALIGGCTNLVLGVISGPTNIPFALVNIVLGLIVGYISKKKGFGYKEAIITGV
                     ILSIVCPLIGTPISVLLFGGLSGSGADLLVGFLVQSGQKIFTAAFFPRILSNIVDKPL
                     SCIMVVYFISKMPKGFIKQYSKNTSSSY"
     gene            3161..3682
                     /locus_tag="KRL67_003623"
     CDS             3161..3682
                     /locus_tag="KRL67_003623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF523 domain-containing protein"
                     /protein_id="HBH1524458.1"
                     /translation="MNRNREIVVVCHCILNCNSKVEGLSEFKGAQNIAIDLINKGYGI
                     IQLPCPEMIMYGINRWGHVKEQFDNLFFREQCRQMLIPHVRQFDEYLKNDYDIKSIIA
                     IDGSPSCGYNKTCSSKRWFGELSGCNELIDKINDIQMVDEKGIFIEELEKLLKEYNLE
                     IPIVGFDELKEDK"
     gene            3853..4026
                     /locus_tag="KRL67_003624"
     CDS             3853..4026
                     /locus_tag="KRL67_003624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH1524459.1"
                     /translation="MTGYVANISQTSQALCGIRSINGTIPVVLTVAMLVLIKMYSLAE
                     RKCREVINELRSR"
BASE COUNT         1781 a          404 c          654 g         1373 t
ORIGIN      
        1 atcatgtatt tactttaata aaataaaaat aggtataaaa aggtatataa taaggctttc
       61 atatacattt atgagtcaaa attttgatat aatatataga tacatggagt tatgaatgga
      121 atttatttaa cttatagggg gagttaatat gaaagcagga gaaattgaat ttcttagtta
      181 tttagagggg tcaaacaaaa gttttgtagt acctgtatat caaagaaatt ataactggaa
      241 gaaagaacaa tgtaaaagat tgtttgatga tttagaggat ataatagatt ctggatttag
      301 aactcatttc ttagggtcag ttgtatctat atatagtatg ggaaaagaat atttaataat
      361 agatggacaa caaagagtga ttactgtgtc aatattattg attgccatgt gtcaaataat
      421 aaaagagttt ggggaatcaa aaggaattat tacaacagaa gaacagatta aggagtgtta
      481 tcttataaac aagcactcaa gagataaaga tggaaaaata aagttaaaac caatagcaga
      541 agacagaaaa gcgtactatg aactatttga agaagaaatg aatacagata gaaattctaa
      601 tatagtttca aattatttat atttttataa tagaataaaa gacgataata tatcaataga
      661 tgatttgttt gatgctatac agaaattaac tatagtggaa atagagttaa aaaatggtga
      721 agatgaccct caattaatct ttgaaagtct aaattcaact ggcttagatt taagtgaagc
      781 tgatagagta agaaactttg tgttaatggg tagaagctct aagttacaag aagaatttta
      841 taataattat tggtataaaa ttgaaaaaaa tacaagtagc aacgtaagta gttttataag
      901 agactatttg actataaagg agagaagtat accgaataaa aataaagtat attttagttt
      961 taaaaggtat gtatcagata atggtataga tatagaaatt ttattaaaag atttacttaa
     1021 attttctaac tattatagag agatatctta ttcatgtact gatgataatg atgtaaacaa
     1081 agttttgaaa aggataaaca aacttgaaat atttgtatca tatccgttta tattagaatt
     1141 atttgatgat tataatgaag gtataataaa taaaaaagta cttgtagaat cccttaattt
     1201 agtagaatgt tttatattaa gaagaatagt atgtgatgta cctccgaatg cactgaataa
     1261 agtatttatg aacttaggaa gggaaattaa aaaatttagt gattataaag aaaactattc
     1321 taatatatta agatatattt ttataaataa gaaatcatct caaagatttc ctactgacag
     1381 tgaattttca aatgcttttg aaactaaaga tatatataat actaagtcaa agaataagtt
     1441 atatatactt gagaggttag agaattataa taataaagaa attgttgatt tagaaaattt
     1501 aatcaataat aatatgctaa atatagagca tataatgcca cagacattaa caaataaatg
     1561 gaaggaatca ctaggagata actataagga gatacatgat aaatatttac atacaattgg
     1621 aaatttaact ttgacaggat ataattcaaa attaagcaat aaaacatttg aagaaaagaa
     1681 agaaatggaa gatggattta aagatagtag gctttatcta aataaatata tttcatctat
     1741 agataaatgg aatgaagaag aaataaaaaa tagaactaag atattactag atagagctgt
     1801 agaaatttgg aaatatccaa gtgttacata tgaacctcca agtaacagtg aaaatatatt
     1861 tacgctagag gatgatgatg ttaattttac aaatgaaagt atagtatcat ttagtatatt
     1921 aggagagtca tataaggcta aaaattggac agaattttat gaacaagttg ctcttacttt
     1981 atatgatttg gattctgtaa ggtttaacag tattgtagac aaaacttata aaagagattt
     2041 tatagataat attaagtcta gagatgtata taaattaaga aaaggtatta aaatatcaga
     2101 aaatatatat ttagaaacaa atttaaatac agaggctaaa ttaaatataa ttagattttt
     2161 acttaatgag tttgatatag agttaaatga agtaatattt tccataaaat aatgaaatat
     2221 atgcgttttg cgcgtaatag aatattaatt atattaaaaa taaaatagaa cctcttgata
     2281 tagatttata gctcaaactt ataccaagag gttacgttta attgaaataa tacaaatact
     2341 atctaattaa attattaata aaaatgttat ataaaaatga gtatatagca ttttaaagag
     2401 taataaatct tacataataa atgatttatt aatatgaact agaagtattt ttactatatt
     2461 gttttataaa acctttaggc attttactta taaaatatac caccataata caagacaaag
     2521 gtttgtctac tatattacta agaatccttg gaaagaacgc agcagtaaat atcttctgac
     2581 cagactgaac taaaaaacca accaataaat cagcaccact tccgcttaaa ccaccaaaca
     2641 ataaaactga aataggagtg ccaattaaag gacaaacaat agataaaatt actcctgtaa
     2701 taattgcttc tttataacca aaaccttttt tcttagaaat atatccaaca ataagaccaa
     2761 gaacaatatt tacaagggca aaaggtatat ttgtaggtcc tgatataaca cctaaaacta
     2821 aatttgtaca cccgccaata agagcacccc aaaaaggacc taacacaaca gccccaagta
     2881 cagtaccaac tgtatctaaa aataataatg gtatatttat catagatata accataccta
     2941 agacaatatt aataactatg cacattgaag aaaatgtgac ttttttagta tccaattata
     3001 atacctccta aaaaaattta tacatttatc atagtataga ttataaaaga ttactaattg
     3061 tatatttcta tatagaatat tggaaatata gaacaaataa ataatataat tatcaaaata
     3121 tgtagtatca ttaaaattaa gaataactaa ggagattatt atgaatagaa atagagagat
     3181 tgtagtggta tgtcattgta tattaaattg taattctaaa gtagagggat tgtcagaatt
     3241 taagggagca caaaatatag caatagattt aataaataaa gggtatggaa taattcaact
     3301 accatgccca gaaatgatta tgtatggaat aaataggtgg ggacatgtaa aagaacagtt
     3361 tgacaatttg ttttttagag aacaatgtag gcaaatgtta atacctcatg taaggcagtt
     3421 tgatgaatat ttaaaaaatg attatgatat aaaaagtatc attgcaatag atggtagtcc
     3481 aagttgtggt tataataaga catgtagttc taaacgctgg tttggtgagt taagtggttg
     3541 caatgaattg atagataaaa taaatgatat acaaatggtg gatgagaaag gtatttttat
     3601 agaagagcta gaaaagctac ttaaagaata taatttagag atacctatag ttggatttga
     3661 cgagcttaaa gaagataagt aaagatataa atatttattg tctcattatg aaacaccatc
     3721 atatttggta gaaaaatatg actgatggac agtacatgaa ccatttttaa gtggaggaat
     3781 tagtactcct aaagaaaaat ttaaaaaaat caatattatc attaatattc atattgatgt
     3841 ttggtactat ttatgacagg atatgttgca aatatttctc aaacttcaca agctttgtgc
     3901 ggtattcgtt ctataaatgg tactatacca gtagtactta cagtagctat gttagtactt
     3961 ataaagatgt attcactagc tgaaagaaaa tgtagagagg taataaatga attaagaagt
     4021 agataatata tataaaggag tgtctaaaag tgaacttttt agtttatgag acactctttt
     4081 tttatattaa atcaaatata ttttaatcgt tttagcaatg aaaaataaat ttatctatat
     4141 tttgagacaa catctttttt agttttattt atttgttgtc gtttttgact tggtatacaa
     4201 ttatatattt tc
//
LOCUS       DAEPXF010000254         4142 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain HB27
            SAMN13376389-rid12479423.denovo.262, whole genome shotgun sequence.
ACCESSION   DAEPXF010000254 DAEPXF010000000
VERSION     DAEPXF010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13376389
            Sequence Read Archive: SRR10518432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4142)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4142)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001069624.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001069624.1
            Genome Coverage       :: 315x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/15/2021 01:54:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,165
            CDSs (total)                      :: 4,076
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 89
            rRNAs                             :: 10, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 10 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 19 of 69
            Pseudo Genes (incomplete)         :: 44 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 10 of 69
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4142
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13376389-rid12479423.denovo.262"
                     /strain="HB27"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="China: Hebei"
                     /collection_date="2017"
     gene            49..1743
                     /locus_tag="KRL67_003625"
     CDS             49..1743
                     /locus_tag="KRL67_003625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HBH1524460.1"
                     /translation="MKFSEFKYERPNYNSIKKEFLGCVEDINNSRNYKEQQKNIHKIN
                     LLRNKIETLSNIASIRYSTDTLNKFYKEEKNYWDEYMPLYEELNSHFYNAIVNSKFKY
                     DLIKEFGEQFFTIVEYSLKSFSKEIISELQEENKLCSEYTRLLASAEIMFDGNIRNLS
                     GMGKFMYSKNRKTRELANKAYYNFFEENETKFDDIFDKLVKLRDKISKKLGFEDFVEL
                     GYVRMMRSDYREDMIKSVRKQVLKYVVPMANELYEKQAKRIGLEYLSYIDEGVEFLTG
                     NASLKGDSRYIIQNGKRMYSELSKETNEFFYFMLENELMDLETKKGKGAGGYCTYIPD
                     YKSPFIFSNFNQTADDIDVLTHEAGHAFQLYMSRWIDMPEINFPTLDSCEIHSMSMEF
                     ITWPWMDLFFKEDTDKYKLTHLSSAIKFMPYGVIVDEFQHYIYKNPNVDKSKRKEIWR
                     FLEKKYLPHRKYGDNSFLERGCWWFKQGHIFKNPFYYIDYVLAQICALQFWKKMIQDR
                     DSGWKDYINICKVGGTKSFLDIVSMGNLYSPFDDGCIESIIGDVKSWFDEINDSKL"
     regulatory      2075..2185
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00504"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="glycine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="glycine"
                     /db_xref="RFAM:RF00504"
     gene            2289..2660
                     /locus_tag="KRL67_003626"
     CDS             2289..2660
                     /locus_tag="KRL67_003626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GrdX family protein"
                     /protein_id="HBH1524461.1"
                     /translation="MIIITNNPKVKEEVQGREVLFKDTTYIGILEASRDLIHEGYELL
                     SHPLYGSVKPNETPYRTVILKKGNRLDINSLTLIEEAIITASKFQNNKKTPKWTESVQ
                     DDFRVIDYDIFYNTIQRMQYE"
     gene            2754..3701
                     /gene="trxB"
                     /locus_tag="KRL67_003627"
     CDS             2754..3701
                     /gene="trxB"
                     /locus_tag="KRL67_003627"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HBH1524462.1"
                     /translation="MENVYDLVIIGSGPAGLAAGLYGARAKLKTLILEKDKTGGQIVI
                     THEIANYPGSVPNATGPSLIARMVEQCKEFGAEMLRDNIVDTELDGDIKVLKGEKAEY
                     RAKAVIIGTGATPRKIGCPGEKELTGKGVSYCATCDADFFEDFEVFVVGGGDSALEEA
                     MYLTKFARKVTIVHRRQGFRCAKSVEEKAKANPKIEFLLDTVIEEIKGDGILESVVFK
                     NKVTGETHEYFADEEDGTMGVFVFVGLDAQTDLFKGKVDMDEKGYIITDEDMRTNIPG
                     VFAAGDCRSKTLRQVVTATNDGAIASIVAEKYIDEKFGN"
     gene            3786..4103
                     /locus_tag="KRL67_003628"
     CDS             3786..4103
                     /locus_tag="KRL67_003628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol reductase thioredoxin"
                     /protein_id="HBH1524463.1"
                     /translation="MLDLDKATFEEEVLNAEGFVFVDFWSEGCEPCKALMPDVHKLAE
                     TYGDKIKFCKMDTTKARRLAIKQKVLGLPTMAIYKDGEKVDEVTKDDATVPNIENMIK
                     KYL"
BASE COUNT         1711 a          393 c          781 g         1257 t
ORIGIN      
        1 tctatattta taaatacaat aaagtaaagt tgtaaaggag tgctttaaat gaagttttca
       61 gagtttaagt atgaaagacc caattataat agtataaaaa aagagttttt gggttgcgta
      121 gaagatataa ataattctcg taattataaa gagcaacaaa aaaatataca taagattaat
      181 ttattaagaa ataaaataga aacgttgtca aatatagcgt cgataagata tagtacagac
      241 acacttaata aattctacaa agaagaaaaa aattattggg atgaatatat gcctttgtat
      301 gaagaattaa attcacattt ttataatgct attgtaaatt caaaatttaa atatgatttg
      361 ataaaagagt ttggagagca atttttcaca atagtagagt attctttaaa aagtttttct
      421 aaagaaataa taagtgaatt gcaggaagaa aataaattgt gttcagagta tactagactt
      481 ttagcttcag ctgagataat gtttgatggg aacataagaa atttatctgg tatggggaaa
      541 ttcatgtatt ctaagaatag aaaaacaaga gaattagcaa acaaagcata ttataatttc
      601 tttgaagaaa atgaaactaa atttgatgac atatttgata aattggttaa acttagagat
      661 aagatatcta aaaaactagg ttttgaagat tttgtagagc ttgggtacgt tagaatgatg
      721 agaagtgact acagagaaga tatgataaaa agtgttagaa aacaagtatt aaaatatgtt
      781 gttccaatgg ccaatgagct ttatgaaaaa caagctaaaa gaattggcct agaatattta
      841 agttacatag atgaaggcgt ggaattttta acaggaaatg caagtctaaa gggagattca
      901 agatacataa ttcaaaatgg taaaaggatg tattctgaac tgtcaaaaga aacaaatgaa
      961 tttttttatt ttatgttaga aaatgaactt atggatttag aaacgaagaa aggaaaagga
     1021 gctggagggt attgtactta tataccagat tataagtcac catttatttt ttctaatttt
     1081 aatcaaactg ctgatgatat agatgtgtta acacatgagg cagggcatgc atttcagttg
     1141 tatatgtcta gatggattga tatgccagaa attaattttc caacgcttga cagttgtgaa
     1201 atccattcaa tgagtatgga atttataaca tggccatgga tggatttatt ttttaaagaa
     1261 gatacagata agtacaaatt aactcactta tcatctgcaa ttaaatttat gccttatgga
     1321 gtaattgttg atgaatttca acactatatt tacaaaaatc ctaatgtaga taagtcaaag
     1381 cgaaaagaaa tctggagatt tttagagaaa aaatacttac ctcatagaaa atatggtgat
     1441 aattcttttt tagagagagg ttgttggtgg tttaaacaag gacatatatt taaaaatccg
     1501 ttttattata tagattatgt attagctcaa atttgtgcgt tacaattttg gaaaaaaatg
     1561 attcaagata gagattcagg atggaaagat tatataaata tctgtaaagt tggaggaaca
     1621 aaatcatttt tagatattgt gagtatggga aatttgtatt ctccatttga tgatggctgt
     1681 attgagtcta ttataggtga tgttaaatct tggtttgatg aaataaatga cagcaaatta
     1741 taaaaaatta tgagaatttt attgtataaa agtgaaaaat taaaaaatac ataaaaagtg
     1801 attatttgtt gtgaaaatag tcttgttttt aaccaaattg ataaaataaa ctaaaaaaga
     1861 tttgtactta aaaaaaaaag aaaatacaag aaatatgtaa tatcaacatt tggaaaaaaa
     1921 agtaaaaatg tatacaaaat acatataaaa aaactattgc aaattttata gaatgtttat
     1981 atacttaagt caacagagat agaaaaaagc tatttataga aaacaaagga taaattgctt
     2041 aattttaaat tttctgaaaa aaataataat ccggatgaag agtatgggag actgctttat
     2101 aaaaagcgac cgaagggaca agtttaatct tacttaccca aaagattaga tgaaattctc
     2161 aggtaaaggt gactgtactt ggacggggct ctgaaaatga ttttaacagg gaagacaaat
     2221 tgggagattt gtttttcctg ttttttattt atcaagtaat aaataaaagt ttgaaagaga
     2281 gtaaaaaaat gataataatt acgaataatc caaaagtaaa agaagaagtt caaggcagag
     2341 aagttttgtt taaagacaca acttatattg gaatcttaga agcaagtaga gatttgatac
     2401 atgaaggtta tgaactttta tctcatccac tctatgggag tgtaaagcca aatgaaacac
     2461 cttacaggac tgttatacta aagaaaggaa atcgtttgga tataaactct ttaaccttaa
     2521 ttgaagaagc aatcatcact gcaagtaaat ttcagaataa taaaaagact ccaaaatgga
     2581 cagaaagtgt tcaagatgat tttagagtaa ttgattatga tatattttat aatacaatac
     2641 aaagaatgca gtatgaataa aaaattgtta aaaagttatt aaaacagctt aaatattaaa
     2701 tctaatttaa tattaaataa aatgagaaat ttaattgggg aggataagtc aaaatggaaa
     2761 atgtgtatga cttagtaata attggttcag gaccagcagg gcttgcagca ggtctttatg
     2821 gagcaagagc aaagttaaaa actctaatcc tagaaaaaga caagactggt ggacaaattg
     2881 taataacaca tgaaattgca aattatccag ggtcagttcc aaatgcaaca ggaccaagtt
     2941 taatagctag aatggttgaa cagtgtaaag agtttggagc agaaatgcta agagacaaca
     3001 ttgttgatac tgagctagat ggagatataa aagttttaaa aggggaaaag gcagaatata
     3061 gagcaaaagc agtaataata ggaacaggag caacaccaag aaagattggt tgtcctggag
     3121 aaaaagaact tactggtaaa ggggtttcat attgtgcaac ttgtgatgca gatttctttg
     3181 aagattttga agtatttgtt gtaggtggag gagacagtgc attagaagaa gcaatgtatt
     3241 taactaaatt tgctagaaaa gtaacaattg tacatagaag acaaggattt agatgtgcta
     3301 agagtgttga agaaaaagca aaagctaatc caaagataga gtttttatta gatacagtaa
     3361 ttgaagaaat caaaggtgat ggaatattag aatcagttgt atttaaaaat aaagttactg
     3421 gagaaactca tgaatacttt gcagatgaag aagatggaac aatgggagta tttgtgtttg
     3481 ttggattaga tgctcaaact gatttattca aaggtaaagt tgatatggat gaaaaaggtt
     3541 acataataac tgatgaagat atgagaacta acataccagg tgtatttgca gcaggagatt
     3601 gtagatctaa gactctaaga caggttgtaa ctgcaactaa tgatggagca attgcatcta
     3661 tagtagctga gaaatacatt gatgaaaaat ttggaaacta gcatctgtaa attaagaaat
     3721 agtgtctgta aattcaatga ataatattta aaattaaaat aaaaatttca agggggaata
     3781 agaaaatgtt agatttagat aaggctacat ttgaagaaga agttttaaat gcagaaggtt
     3841 ttgtatttgt tgatttttgg agtgaaggtt gtgagccttg taaagcttta atgccagatg
     3901 ttcataaatt agctgagact tatggagata aaataaaatt ctgtaagatg gatacaacaa
     3961 aagctagaag attagctata aaacaaaaag tattaggtct tccaactatg gctatataca
     4021 aagatggaga aaaagtagac gaagttacta aggatgatgc tacagttcct aatatagaaa
     4081 acatgattaa aaaatatctt taatatgttg ataaagtatt aacattaaaa gaaaaaatct
     4141 at
//
LOCUS       DAEPXF010000255         4121 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain HB27
            SAMN13376389-rid12479423.denovo.263, whole genome shotgun sequence.
ACCESSION   DAEPXF010000255 DAEPXF010000000
VERSION     DAEPXF010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13376389
            Sequence Read Archive: SRR10518432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4121)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4121)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001069624.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001069624.1
            Genome Coverage       :: 315x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/15/2021 01:54:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,165
            CDSs (total)                      :: 4,076
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 89
            rRNAs                             :: 10, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 10 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 19 of 69
            Pseudo Genes (incomplete)         :: 44 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 10 of 69
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4121
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13376389-rid12479423.denovo.263"
                     /strain="HB27"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="China: Hebei"
                     /collection_date="2017"
     gene            35..1342
                     /locus_tag="KRL67_003629"
     CDS             35..1342
                     /locus_tag="KRL67_003629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH1524464.1"
                     /translation="MKEIADAITSEYLSYGINEDISIYEGRFCIYLDKKYRCNGKIYY
                     KMTPPISISFKAEIGCVEEIDNEDDNLALDYDNAILEVHGYKIISITINTLSEFSVEG
                     YINDDCIKSKNSYVEYVDFNIINLDKIPGKLIKYNDKVYAGRIEFDINDYVVTIDKRY
                     DYRKELKSELKSKSGAIITHIGRIRRKDGRIFRTNNTINLLDRISTALSFMCGRYVGF
                     CLAKGYRSGNEVYRIWNENQISPFRYVPTWSDTLSNYHNMEKYISLMCKKLEDFYYGS
                     AIKSVVDWYIESLGSATMENNIISVQIALETLSYVILVEQSKILTDEAFDCNLTSKNI
                     RLLLDTCKIPYGKHELNIFDNIIKNKFDDGVDLVIYLRNSIVHPSRKTHRAVLEVEDI
                     WNIISIGTRYIELVLLFILGYRGEYSNRLVERCYGEVEVVPWN"
     misc_binding    1428..1690
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            1816..2619
                     /locus_tag="KRL67_003630"
     CDS             1816..2619
                     /locus_tag="KRL67_003630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HBH1524465.1"
                     /translation="MKNILKKVGIFTIMLGLLGGVVGCSKPDNEKDKDASKKSKKEVV
                     VGFDNTFVPMGFLDEKGDTVGFDVDLAKETFKRLGMEVKFQPIDWSMKETELNDSKTV
                     DVLWNGYSITDERKKIVSYTEPYLQNKQIIVTLSDSKINSKADLKDKEVGTQQGSTAL
                     DAVEKDKDFMNSLKGGAPVLYDTYDKALRDLEIGRTSAVVGDEVLIRYYMGQKGEDKY
                     KVLKDDFGLEDYVVATSKENPELCEKINETLKEMKKDGTFDKIYDKWFK"
     gene            2657..3268
                     /locus_tag="KRL67_003631"
     CDS             2657..3268
                     /locus_tag="KRL67_003631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HBH1524466.1"
                     /translation="MLQGLEIVIAMFCITLIVSIPLGIGVAFLRLSKNKLVSGITQCY
                     ILIMRGTPLLLQMIVIFYGLPLLGIVFDRFTAGVVAFFLNYAAYFAEIFRGGIQSIDR
                     GQYEASKVLGFDKFTMYKRVIFPQVFKRILAPISNEVITLVKDTSLVYILGLNDILRI
                     SQIAMNREASLLPLFEAGAIYLIFVAILTKGFELLEKKYSYYR"
     gene            3300..3971
                     /locus_tag="KRL67_003632"
     CDS             3300..3971
                     /locus_tag="KRL67_003632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HBH1524467.1"
                     /translation="MLKIKNLNKSFKKNRVLKDISFELEEGQIGVLLGKSGAGKTTIL
                     RCINGLEEFDSGEIIIDNEVIKNKRDMIKIRGKIGMVFQNFNLFPHMTVLENIIESPV
                     NVFKVPRKEAEERARELLRLVDLEDKLNSYPFELSGGQQQRVAIARSCALMPKVLCFD
                     EPTSALDIDTIQRVVNIMNRLKDKGMTILIITHDVVFSNNVADKIISIKDGIVENVQI
                     KEKIV"
BASE COUNT         1632 a          399 c          762 g         1328 t
ORIGIN      
        1 tgagttttac ataaaagcaa aggtgggaat aaaaatgaaa gaaattgctg atgctataac
       61 atcagagtat ttaagctatg gtatcaatga agatattagt atctatgaag gtagattttg
      121 catatattta gataaaaaat atagatgtaa tgggaaaatt tattataaaa tgacacctcc
      181 gatatctata agttttaaag cagagatagg ttgtgttgaa gagatagaca atgaagatga
      241 caatttagct ttagattatg ataatgctat tcttgaggtt catggatata aaattatttc
      301 tataacaata aatactttaa gtgaatttag tgtagaaggc tatatcaatg atgactgcat
      361 aaaatctaag aactcctatg ttgaatatgt agattttaat ataataaact tagataaaat
      421 acctggaaaa ctcattaaat acaatgataa ggtatacgca ggtagaattg aatttgatat
      481 aaatgattat gttgtaacta tagacaagcg atatgattat agaaaagaat taaaatcaga
      541 attaaagtct aaatctggag caataataac tcatataggt aggattagaa gaaaagatgg
      601 tagaattttt agaacaaata atacaataaa tcttttagat agaatatcaa cagctttgag
      661 ttttatgtgt ggaagatatg tagggttttg tttggccaaa gggtatagaa gtggtaatga
      721 agtttataga atatggaatg aaaatcaaat atctcctttt agatatgtac caacatggtc
      781 agatacactt tcaaattatc ataatatgga aaaatatata agccttatgt gtaaaaagct
      841 agaagatttt tattatggtt ctgctataaa gagtgttgtt gattggtaca tagaatctct
      901 tggaagtgct accatggaaa acaatataat atctgtacaa attgcacttg aaactttgtc
      961 atatgtaatt ctagtagaac aaagtaagat acttacagat gaagcatttg actgtaattt
     1021 gacatctaaa aatataagac tcttacttga tacatgtaag ataccatatg gtaaacatga
     1081 attaaacata tttgataata taataaaaaa taaatttgat gatggagtgg atttagttat
     1141 ttatttaaga aatagcatag ttcatccaag cagaaagact catagagctg tgttagaggt
     1201 tgaagatatt tggaatataa tttcaatagg aacgagatat attgaacttg ttttactctt
     1261 tattttaggt tatagaggag aatattcaaa cagattagta gaaaggtgtt atggtgaagt
     1321 ggaagttgta ccttggaact agatttttat atttatttta aaatatatat tgacatatat
     1381 tgccttatgc tataaaatca atatcataat aataattcac aataacatta aatgctaaga
     1441 aaagaaatag taaaagtagt taatcttaaa cagagagttt ccgattggtg agaggagcaa
     1501 tagattttac tttgaacaca tctttgagca ggactctgaa agagtagaaa tactttatta
     1561 ggagatttcc ggaatccttg cacccgttat agtgctagag tataatgatt ttattgtact
     1621 taaagaggtt agtgtcgtga ggcattaatg aacagaggtg gaaccacgag agttaactcc
     1681 cgtcctctag cagaagctag aggcgggagt tttttaatat aattttttaa gcgcgcagag
     1741 aattatatta caatagatac actttttaaa aaataatcaa aatattttaa aaattaaaag
     1801 gcgagggaga atattatgaa aaatatatta aaaaaagtag gaatatttac aattatgtta
     1861 ggtttattag gtggagttgt aggttgttca aaaccagata atgaaaaaga taaggatgct
     1921 tcaaagaaat ctaaaaaaga agtagttgta gggtttgata atacctttgt accaatggga
     1981 tttttagatg aaaagggaga tacagtagga tttgatgtag acttagctaa agaaacattt
     2041 aaaaggcttg gaatggaagt taagtttcag cctatagatt ggtcaatgaa ggaaactgaa
     2101 ttaaatgatt caaaaactgt agatgtatta tggaatggat attctataac agacgaaaga
     2161 aagaaaatag tatcatatac agaaccatat ttacaaaata agcagataat agttactcta
     2221 agtgattcaa aaataaattc aaaagctgat ttaaaagata aagaagttgg aacacaacaa
     2281 ggttcaacag cacttgatgc agtggaaaag gataaagatt ttatgaatag cttaaaaggt
     2341 ggagcaccag ttctttatga tacttatgat aaagcactta gagatttaga gataggtaga
     2401 actagtgcag ttgttggtga tgaagtttta attagatact atatgggaca aaaaggtgaa
     2461 gacaaataca aggtattaaa agacgatttt ggattagaag attatgttgt agcaacttct
     2521 aaagaaaatc cagaactttg tgagaaaata aatgaaacat taaaagaaat gaaaaaagat
     2581 ggaacttttg acaaaattta tgataagtgg tttaaataga agataagtta gctgttaaga
     2641 agggggcact aaaggcatgt tacaaggttt agagatagtt atagcaatgt tttgtataac
     2701 attgatagta tcaattccat tggggattgg agttgcattt ttaagattat caaagaataa
     2761 attagtaagt ggtattacac aatgttatat attgataatg agaggtacac ctttactttt
     2821 gcaaatgata gtcatatttt acggattacc tcttttagga attgtatttg acagatttac
     2881 agctggagta gtagctttct tcttaaatta tgcagcatat tttgcagaaa tatttagagg
     2941 tggaatacag tcaatcgata gaggacaata tgaagcttca aaagtactag gatttgataa
     3001 atttactatg tataagagag taatctttcc acaagttttt aaaagaatac tagcacctat
     3061 ttcaaatgaa gtaatcacat tagtaaaaga tacatcttta gtatatatat taggattaaa
     3121 tgatatttta agaatatctc aaatagcaat gaacagggaa gcaagtctgt taccattatt
     3181 tgaggcagga gcgatatatc ttatatttgt agctatactt actaagggat ttgaattact
     3241 tgaaaaaaaa tattcttatt atagataaag ttacaatatt ataggaggag gttttttata
     3301 tgttgaaaat caaaaactta aataaatcat ttaaaaaaaa tagagtactt aaagatattt
     3361 cttttgagtt agaagaaggt cagattggag ttttacttgg taagtctggt gcaggaaaga
     3421 ccacaatatt aagatgtata aatggtcttg aagaatttga tagtggagag attataatag
     3481 ataatgaggt aataaaaaat aagagagata tgataaaaat acgaggaaaa ataggtatgg
     3541 tttttcaaaa tttcaactta tttcctcata tgacagtcct tgaaaatata attgaatccc
     3601 cagttaatgt ttttaaagtt cctagaaaag aggctgaaga gagggctaga gaacttttaa
     3661 gattagttga tttagaagat aaattaaatt cttatccatt tgaattgtca ggaggacagc
     3721 aacagagagt tgctatagct aggtcatgtg cattgatgcc aaaagtcctt tgttttgatg
     3781 aacctacttc tgcacttgat attgatacta ttcaaagagt agtcaatata atgaacagac
     3841 ttaaagacaa aggtatgaca atattaataa ttacacatga tgttgtcttc tcaaataatg
     3901 ttgcagataa aattataagt ataaaagatg gtatagtaga aaatgttcaa ataaaagaaa
     3961 aaattgttta gagttttata aatataaact agggtagaat tttataatat aaataaaggg
     4021 caagtaaaat caaatatact atgccctttt gttatctcaa aatttaatct tatattttat
     4081 gtttaagtta cattataatt tataaagtaa gctatattta a
//
LOCUS       DAEPXF010000256         4103 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain HB27
            SAMN13376389-rid12479423.denovo.264, whole genome shotgun sequence.
ACCESSION   DAEPXF010000256 DAEPXF010000000
VERSION     DAEPXF010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13376389
            Sequence Read Archive: SRR10518432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4103)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4103)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001069624.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001069624.1
            Genome Coverage       :: 315x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/15/2021 01:54:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,165
            CDSs (total)                      :: 4,076
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 89
            rRNAs                             :: 10, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 10 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 19 of 69
            Pseudo Genes (incomplete)         :: 44 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 10 of 69
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4103
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13376389-rid12479423.denovo.264"
                     /strain="HB27"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="China: Hebei"
                     /collection_date="2017"
     gene            5..808
                     /locus_tag="KRL67_003633"
     CDS             5..808
                     /locus_tag="KRL67_003633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH1524468.1"
                     /translation="MEKLRVIEMIKNNPNIIATIDNPTDEMKLLAIKENGLVLEYIDN
                     PTREMQELAIDNNIRAIKFIDNPTEDMMIKAVNEGWSILDYIKNPTDKVIEIAINQAG
                     WAIKYAKNPSEELQLLAVRKNYDSIKFIKQPYESVQEEAVRISYDALRYIDSPSYNAE
                     LIAIKNNEAAISFINDLDKNKMLEFLKVNILVIKYILKKVSKDELESVLKEVLSKEDV
                     EEKYIRDFLNCSVIDRNSESIPIDKIMFIYKYGSKTAKKIAVDEKLKIM"
     gene            820..1950
                     /locus_tag="KRL67_003634"
     CDS             820..1950
                     /locus_tag="KRL67_003634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBH1524469.1"
                     /translation="MNFIDTLKSVDLILSTGEVPLIVGESGIGKTALANKLAKENDWS
                     LIVIDGNLLKEGEIGGLPTIESYVGVNSNGYKTEKKTTVYAVHNKLREIDEEISKSKT
                     VLLFIDEINRCEHTVQQELMNLILNREINGYKLHDDVKILAAMNPSSKYGSDFDYQVV
                     DMDAAQENRFVWLNMESEHTQWIKWAIDEGIERKVIEFISTFPEYLHKINEDDVRATP
                     RSYERVSKIYKVYKEKNNSIPRAVFLNVVKGNVGKVIAEEFISFIESNSEPLISYEDV
                     FLGESIDESIVERVKNESHTRLYLSAMNILKDLELNIKNDKYESNNYINRFIEFLKMY
                     PVDLMIGIMKDIRNSYIEVYKKAIENEEFVKSYFESYSLIRG"
     gene            1954..3363
                     /locus_tag="KRL67_003635"
     CDS             1954..3363
                     /locus_tag="KRL67_003635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH1524470.1"
                     /translation="MENYFDKQAKELYNKASEIINTHSMLKANRANEKFDIDIPQDFK
                     YEFFSLVDKVNLSLMEEEDNFYGYFLFQMLREIRFDISSPTGVNFKGAKYVIYFNPII
                     FLTLDIKQMETTIKHEIHHILSMHLMRAKDLKGKYSTLAINMAMDIVVNKFLNNLPPY
                     ATTLEWVNLKYSLKLEPYEPFEYYVEKIQTELDLMEVDEDGEEDDSDKYGDIEIDYSP
                     ERTHDIWDESNEIDENTLREFTEKFINSSQKGKVPAYLESMISSLKNSKGELPWNLYL
                     NRIMGTVENNKKKTITRRNRRQPNRLDLRGELRSHKAEIAVALDVSGSISDEEFKQAI
                     KEVLSIVKNYNHEVTIIECDNEIRRVYKVKTEKDIKDRIRIGGGTRFSPVFEYANNKK
                     INLLVYFTDGKGESKLQVIPRGYKILWVISGRGDKISLKEPYGAVKKLTKVKTNDEIL
                     EINDVRNDGYSMNSQAPIL"
     gene            3574..4083
                     /locus_tag="KRL67_003636"
     CDS             3574..4083
                     /locus_tag="KRL67_003636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CarD family transcriptional regulator"
                     /protein_id="HBH1524471.1"
                     /translation="MVMFLFKIDDYIMYGMTGVCKVMDITNERFTNGIKKEYYVLSPI
                     YSNNTVIKIPVDNEKVPMRKLLSKINVLSLINDIPNMDTSWIDNEKLRSEQFKKILRG
                     GKCEELIKLVRSIDNNREYVKSIGKKTHQADDNIMKEAERLLNEEFATILDISPNEVS
                     SYISSHIPQ"
BASE COUNT         1712 a          361 c          748 g         1282 t
ORIGIN      
        1 cattatggaa aaattgagag ttattgaaat gataaaaaat aatcccaata ttatagctac
       61 aatagataat ccaactgatg aaatgaaatt gttagctatt aaagaaaatg ggcttgtact
      121 agaatatata gataatccaa ctagagagat gcaagaacta gcaatagata ataatattag
      181 agcaattaaa tttatagata atccaacgga agatatgatg ataaaagcag taaatgaggg
      241 atggagtata ctggattata tcaaaaaccc aactgataaa gttattgaaa ttgctataaa
      301 tcaagcagga tgggcaatta aatatgctaa aaatccaagt gaagaattac aactattagc
      361 agtaagaaaa aattatgatt cgataaagtt tataaaacaa ccttatgaaa gtgtacaaga
      421 ggaggcagtg agaataagtt atgatgcctt gagatatata gattcaccta gttataatgc
      481 agaacttata gctataaaaa ataatgaagc agctattagc tttataaatg atttagataa
      541 aaataaaatg ttggaatttc taaaagtgaa tattttagtg attaagtaca ttctgaaaaa
      601 agtatctaaa gatgaattag agagtgtatt aaaagaagta ttatccaaag aagatgttga
      661 ggaaaagtat attagagatt ttttgaattg tagtgttata gatagaaata gtgaaagtat
      721 accaatagat aaaattatgt ttatttataa atatggaagt aagacagcaa aaaaaatagc
      781 tgtagatgag aaattaaaga ttatgtagga gagagaaaaa tgaattttat agacacatta
      841 aaaagcgttg acttaatatt atccactgga gaagtacctt taatagtagg tgaaagtgga
      901 ataggaaaaa cagctttagc aaataagctt gccaaagaaa atgattggag tttaattgtt
      961 attgatggaa acttacttaa agagggtgaa ataggaggtc ttccaactat agaatcttat
     1021 gtaggagtta attctaatgg atataagact gaaaagaaaa caacagtgta tgctgttcat
     1081 aacaaattaa gagaaattga tgaagagata tctaaaagta agacagttct tttatttata
     1141 gatgagataa accgttgtga gcatacagta cagcaagaac ttatgaattt aattttaaat
     1201 agagaaatta atggatataa actgcatgat gatgtaaaga ttttagcagc aatgaaccca
     1261 tcaagtaaat atggttcaga ttttgattat caagtggtag atatggatgc agcccaagaa
     1321 aataggtttg tttggttaaa tatggagagt gagcatacac aatggataaa atgggcaata
     1381 gatgagggta ttgaaagaaa agttatcgag tttatttcca cttttccaga atatctgcat
     1441 aaaataaatg aagatgatgt aagagctact cctagaagtt atgagagagt atctaaaatt
     1501 tataaagttt ataaagaaaa aaataattct attcctagag cagtattttt aaatgttgta
     1561 aagggtaatg taggtaaagt tattgcagaa gaatttataa gttttattga atcaaactct
     1621 gaaccattaa tatcatatga ggatgttttt ttaggagaat ctattgatga gtccattgta
     1681 gaaagagtaa aaaatgagag tcatacaaga ctttatctat cagctatgaa cattctaaaa
     1741 gacttagaat taaatattaa gaatgataaa tatgaatcaa acaactatat taatagattt
     1801 attgaatttt taaaaatgta tcctgtagat ttaatgatag gtattatgaa ggatattaga
     1861 aacagttata ttgaagtata taaaaaagct atagaaaatg aagagtttgt aaaatcatat
     1921 tttgaatctt atagtttaat aaggggataa tgtatggaaa attattttga taaacaagca
     1981 aaagagcttt ataataaagc aagtgaaatt ataaatactc attctatgct aaaggcaaat
     2041 cgtgctaatg agaaatttga tatagatata ccacaagatt ttaagtatga gttttttagt
     2101 cttgtagata aagtgaattt aagtcttatg gaagaagaag ataattttta tggatatttt
     2161 ttgtttcaga tgttaagaga aataagattt gatataagta gtcctacagg tgtgaatttt
     2221 aagggagcta aatatgttat atattttaat ccaataatct ttttaacgct tgatataaag
     2281 caaatggaga ctacaattaa acatgaaatt catcatatat tatctatgca tctgatgaga
     2341 gcaaaggatt taaaaggaaa gtatagtacc ttggcaatta atatggcaat ggatatagta
     2401 gtaaataaat ttctaaacaa tttaccacca tatgcaacta ccttagagtg ggtaaattta
     2461 aaatattcct taaaacttga accatatgaa ccttttgaat attatgtaga aaagattcag
     2521 actgaattag atttgatgga agtggatgaa gatggtgagg aagatgatag tgataaatat
     2581 ggagatatag aaattgatta tagccccgaa agaactcatg atatatggga tgaatccaat
     2641 gagatagatg aaaataccct tagagaattt actgaaaaat ttattaacag ttctcaaaaa
     2701 ggtaaagttc ctgcttattt agaaagtatg atatcatcac ttaaaaatag taagggtgaa
     2761 ttgccatgga atttatatct taatagaata atgggaacag ttgaaaataa taaaaagaag
     2821 actataacaa gaagaaatag aagacaacca aatcgtttag atttgagagg agaacttaga
     2881 agtcataagg cagaaatagc agttgcactt gatgtaagtg gaagtattag tgatgaagag
     2941 tttaaacaag cgattaaaga agttcttagt atagtgaaaa actataatca tgaagttaca
     3001 attatagaat gtgataatga aattagacgt gtatataaag tgaaaactga aaaagatata
     3061 aaagatagaa ttaggatagg tggaggtaca agattttctc cagtatttga gtatgctaat
     3121 aataaaaaga ttaatttgtt agtctacttt actgatggta aaggtgaaag taagttacaa
     3181 gtaataccta gagggtataa aattttatgg gttatttctg gaagaggaga taaaatttca
     3241 ttaaaagaac cttatggagc agtaaaaaaa cttactaaag ttaaaacaaa tgatgaaata
     3301 ttagaaataa atgatgtaag aaatgatggg tactcaatga atagccaagc accaatttta
     3361 taggtattca caggagataa gctgtcttaa tttattttga tatgcttgtt ataaaaatct
     3421 aaaaaatgca ttaatcaatg gttaatgtat tttttaattt taataatata agttttactt
     3481 acattaaagt ctcaaaatgt gttaaaatat aatatatagc agatagtttt ggcaaggtgg
     3541 atttagttgt caaaactatt ttttttatta aggatggtga tgtttttgtt taagattgat
     3601 gattatataa tgtatggtat gacaggagtc tgcaaagtca tggatataac aaatgaacga
     3661 tttacaaatg gaataaaaaa agagtattat gtattgagtc ctatttattc taataataca
     3721 gtaataaaaa ttcctgtaga taatgaaaaa gtaccaatga gaaagttgct ttctaaaatt
     3781 aatgtgcttt cattaataaa tgatattcct aatatggata catcatggat agataatgaa
     3841 aaattgagaa gtgagcaatt taaaaaaata ctcagaggtg gtaaatgtga agagttaata
     3901 aaattagtaa gaagtataga taataacaga gagtatgtaa aatctattgg taaaaagaca
     3961 catcaagcag atgataatat aatgaaagaa gctgaaaggt tattaaatga agaatttgca
     4021 actattttag atatttctcc taatgaagtt agttcatata tttcaagtca tatacctcaa
     4081 taaagtgttt tagtatcctt tat
//
LOCUS       DAEPXF010000257         4044 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain HB27
            SAMN13376389-rid12479423.denovo.265, whole genome shotgun sequence.
ACCESSION   DAEPXF010000257 DAEPXF010000000
VERSION     DAEPXF010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13376389
            Sequence Read Archive: SRR10518432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4044)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4044)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001069624.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001069624.1
            Genome Coverage       :: 315x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/15/2021 01:54:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,165
            CDSs (total)                      :: 4,076
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 89
            rRNAs                             :: 10, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 10 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 19 of 69
            Pseudo Genes (incomplete)         :: 44 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 10 of 69
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4044
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13376389-rid12479423.denovo.265"
                     /strain="HB27"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="China: Hebei"
                     /collection_date="2017"
     gene            184..1137
                     /locus_tag="KRL67_003637"
     CDS             184..1137
                     /locus_tag="KRL67_003637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HBH1524472.1"
                     /translation="MKKRYLVIGIIILSYFSLFIGAEDINIMHIFAKNQHKLMIFIMS
                     RVPRLISILIAGVGMSVAGLIMQQISKNKFVSPTTGATIDAAQFGIVICMLLVPTASI
                     FTKTIIAFVFSLVGTFMFMKIIGKLQFKNIIFVPLVGIMFGNIIGSMTDFIAYKYDLS
                     QNVSSWMQGDFSMILKGNYEILYITIPLIILAYIYANKFTVVGMGMDFATNLGLSYKR
                     IVNIGLIIVALVTVCVVVTAGNIPFIGLIVPNIVSLYMGDNIRASIWYTGLLGAIFVL
                     ICDIFGRIIIYPYEISIGLTVGVIGSILFLYLILRRNVNEA"
     gene            1127..2083
                     /locus_tag="KRL67_003638"
     CDS             1127..2083
                     /locus_tag="KRL67_003638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HBH1524473.1"
                     /translation="MKLSLNQKIYFISILLFISIALFVIYEIDMNHLEYALSQRIPKI
                     FAMVLGGGCIAFTTVVFQTITNNQILTPSVLGLDSLYVMIQTIIVFIFGSSSSLIINE
                     NYNFIINVAMMIGASLLLYKTLFEKNKNNIFFLILVGMIFGTLFKSATTFIQVMIDPN
                     EFLALQTSIMASLNNINTNVLLIAFIIIIAIIPFIYDEIKYLDVLSLGKEQAINLGVD
                     FDKAVKKMIILIAILVSISTALIGPMTFLGLLLANITREIFKTYKHTYLISGSMLIGI
                     ITLIIGQFFIQHVFKFDTTLSVVINFIGGIYFIQLLLKGANR"
     gene            2080..2835
                     /locus_tag="KRL67_003639"
     CDS             2080..2835
                     /locus_tag="KRL67_003639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003234487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBH1524474.1"
                     /translation="MIEIKNIFKKYKNKNVVDDVSFSIEKGKITSFIGPNGAGKSTVL
                     SIVTRLIGGDGGEVIIEGKSLTNYSNKELAKKIAILKQSNNITLKLTIRELVGFGRFP
                     YSEGNLTEEDENCIDEAIEYMKLTDIQHKYLDELSGGQRQRAYIAMVIAQDTEYILLD
                     EPLNNLDMNHSVQMMKVLRGLCDELDKTIVLVMHDINFASCYSDNIVALKNGKVEKVG
                     RTDEIVNEKVLEDIYEMNFNIKNINGNRICIYF"
     gene            2927..3892
                     /locus_tag="KRL67_003640"
     CDS             2927..3892
                     /locus_tag="KRL67_003640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
                     /protein_id="HBH1524475.1"
                     /translation="MNKKAAIVAAIAIIGLVTVFALGGSKKNESKTSENSNNTIKITH
                     NLGETDVKLNPKKVVVFDYSALDTMDALGVAENLVGLPKASLPASLEKYKDEKYADLG
                     GLKEPDLEGIKSANPDLIIINGRQEDFYEQLSKIAPTISTSKDDKKYLESVKNNIDKI
                     AKIFEVEEKANQEFSKIEKKIETLNKKVTDKNLNALTIMVNEGNLSVFGEESRFSILY
                     NSFGFENKDKNIKESSHGQNITFEYIAKQNPEVMFVIDRGIATGSDVKESSTAKSVLN
                     NDIIKSMDAYKNDNIIYLDSPTWYVNDGGLTSLNKMIDDASKAVN"
BASE COUNT         1630 a          384 c          675 g         1355 t
ORIGIN      
        1 tggtgttcat aatttattaa attaaatttt aatgttacta gaagttttaa ttctagtaac
       61 attttttatg ctaaaaacag atataaatat atattgacaa tgataattat tattgataaa
      121 ctataaaagt gaaaatgata ataattttca ttttaattaa taaaagattt ttaaggaggg
      181 agaatgaaaa aaaggtattt agtcatagga ataataattt tgtcttattt ttctttattt
      241 ataggagctg aagatataaa tataatgcat atatttgcaa aaaaccagca taagcttatg
      301 atatttatca tgagtagagt tccgagatta ataagtatac tgattgctgg tgtagggatg
      361 agtgtagcag gtcttataat gcaacagata agtaaaaata aatttgtatc acctacaaca
      421 ggagctacaa tagatgcagc tcaatttggg atagtaatat gtatgctttt agtaccaact
      481 gcctctattt ttacaaagac aataatagca tttgtatttt ctcttgtagg tacttttatg
      541 tttatgaaaa taataggaaa gttgcaattt aaaaatataa tctttgtacc attagtaggt
      601 ataatgtttg gaaatataat aggttctatg acggacttta tcgcatataa atacgatttg
      661 agtcaaaatg ttagttcttg gatgcaaggt gacttttcta tgatacttaa aggaaattat
      721 gagatattat atatcacaat accccttata atattagcat atatttatgc aaataaattt
      781 actgttgttg gtatgggtat ggattttgca acaaacttag gtcttagtta caaaagaatt
      841 gtcaatatag gtttgataat agtagcactt gttacagtat gtgtagtcgt tacagctggt
      901 aatatccctt ttataggcct aatagttccc aatatcgtat ccttgtatat gggtgataat
      961 atacgagcaa gcatatggta tactggttta cttggagcga tatttgtact tatatgtgat
     1021 atttttggaa gaatcattat ttatccatat gaaatatcta tagggcttac agttggagtt
     1081 ataggaagta tactattcct ttatttaata ttaaggagga acgtaaatga agcttagtct
     1141 caatcaaaaa atttacttta taagtatatt actattcata tcaatagcac tgtttgtaat
     1201 ttatgagata gacatgaatc atttagaata tgcattatca caaagaatac caaaaatatt
     1261 tgctatggta ttaggtggag gatgtatagc atttactact gtagtgtttc aaacaattac
     1321 aaacaatcaa atattaacac caagtgtact aggtttagat tcattatatg taatgattca
     1381 aactataata gtattcatat ttggttcaag cagtagtctg attataaatg aaaattataa
     1441 ttttattatt aatgtagcta tgatgattgg agcttcattg ttattatata agacgttatt
     1501 tgaaaaaaat aaaaataata ttttcttttt aatcctagta ggaatgatat ttggaacctt
     1561 atttaaaagt gcaacaacat ttattcaagt aatgatagac ccgaatgaat ttttagcttt
     1621 gcaaactagt ataatggcca gtttaaacaa tataaacact aatgtattgt taatagcatt
     1681 tataataatt attgccataa taccttttat atatgatgaa ataaagtatt tagatgtatt
     1741 gtcgttaggt aaagaacaag caattaattt aggtgtagat tttgataaag cagttaaaaa
     1801 aatgataata ttaatagcaa ttttagtctc aatatcaaca gcacttattg gacctatgac
     1861 atttttagga cttttacttg ccaatataac aagagaaatt tttaaaacat ataagcatac
     1921 atacttgatt tcgggttcta tgttgatagg gataattaca ttgataatag gtcaattttt
     1981 tatacaacat gtatttaagt ttgatacaac tttaagtgta gtaataaatt ttataggagg
     2041 aatttacttt attcaattac tattaaaggg ggcaaataga tgatagagat aaaaaatata
     2101 tttaaaaaat ataagaataa aaatgttgta gatgatgtgt cttttagtat agaaaaggga
     2161 aagataacat cttttatagg accaaatgga gctggaaaaa gtacagtact atcaatagtt
     2221 acaaggctta taggaggtga tggtggagaa gtaataatag aaggaaaaag cttgactaat
     2281 tatagcaata aagaattagc aaaaaaaata gctattctta agcaatctaa caatataact
     2341 ttaaaactta caataagaga attagttgga tttggaagat ttccatatag tgaaggtaat
     2401 ctaactgaag aggatgaaaa ttgtattgat gaagcaatag aatatatgaa gttgactgat
     2461 atacaacaca agtatttaga tgaattgagt ggaggtcaaa ggcaaagagc ttatatagca
     2521 atggtaatag ctcaagatac agagtacata ttacttgatg aaccactgaa caatttggat
     2581 atgaaccatt cagtacagat gatgaaggta cttagaggtc tttgtgatga gttagataag
     2641 actatagtat tagtaatgca tgatataaat tttgcatcat gttattcaga taatatagtt
     2701 gcattaaaga atggaaaagt tgaaaaagta ggtcgtacag atgagatagt aaatgaaaaa
     2761 gttttagaag atatttatga gatgaatttt aacattaaga atataaatgg aaatcgaatc
     2821 tgtatttatt tttaaaatat tgtatataaa aataagcttg ctatatgatt ttttgtaaaa
     2881 tttttaaatt aaaataaaga ttaataggag agaggaaaat attgatatga ataaaaaagc
     2941 agcaatagta gcagcaatag ctattatagg attagtgact gtgtttgcat taggtggaag
     3001 taaaaagaat gaatcaaaaa catcagaaaa ttcaaataat actataaaga taactcataa
     3061 cttgggagaa acagatgtaa aattaaatcc taaaaaggta gtagtatttg actattcggc
     3121 tttagataca atggatgcat taggtgtagc tgagaattta gtaggacttc caaaagcgag
     3181 tttaccagca tctttagaaa aatataagga tgagaagtat gcagatttag gaggcttaaa
     3241 agaaccagat ttagaaggta taaagtcagc aaatccagat ttaattataa taaatggaag
     3301 acaagaagat ttttatgagc aattgtcaaa aatagcacca acaataagta caagtaaaga
     3361 tgataagaag tacttagaat ctgttaaaaa taatatagat aaaatagcaa aaatatttga
     3421 agtagaagaa aaagcaaatc aagaatttag taaaattgaa aagaaaatag aaactttgaa
     3481 taaaaaagta acagataaaa atttaaatgc tttaactata atggtaaatg aaggtaattt
     3541 aagtgtattt ggtgaagaat caagatttag tatattatac aatagttttg gatttgaaaa
     3601 taaagataaa aatataaaag aatcaagtca tggacaaaat attacttttg agtacatagc
     3661 taaacaaaat ccagaagtta tgttcgttat agatagagga atagctactg gtagtgatgt
     3721 aaaagaaagc tcaactgcaa agtctgtttt aaacaatgat ataataaaat ctatggatgc
     3781 atataagaat gataatataa tatatttaga ttcaccaaca tggtatgtaa atgatggtgg
     3841 tctgacatct ttaaataaaa tgatagatga tgcttcaaaa gcagtaaatt aataatccga
     3901 tataatcaaa atgtattttt tgtaaaatga ttatcaaata aactaggagt ttttaagaac
     3961 tcctagttta ttttttgcat aagaaaaata taatttaaac aataatatta tttacaataa
     4021 gataaatcta ttcaaaatct aata
//
LOCUS       DAEPXF010000258         4026 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain HB27
            SAMN13376389-rid12479423.denovo.266, whole genome shotgun sequence.
ACCESSION   DAEPXF010000258 DAEPXF010000000
VERSION     DAEPXF010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13376389
            Sequence Read Archive: SRR10518432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4026)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4026)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001069624.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001069624.1
            Genome Coverage       :: 315x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/15/2021 01:54:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,165
            CDSs (total)                      :: 4,076
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 89
            rRNAs                             :: 10, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 10 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 19 of 69
            Pseudo Genes (incomplete)         :: 44 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 10 of 69
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4026
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13376389-rid12479423.denovo.266"
                     /strain="HB27"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="China: Hebei"
                     /collection_date="2017"
     gene            177..1187
                     /locus_tag="KRL67_003641"
     CDS             177..1187
                     /locus_tag="KRL67_003641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4116 domain-containing protein"
                     /protein_id="HBH1524476.1"
                     /translation="MHEDKNDIFESIDTEDFDNKTEEECLELVRKNGLNLIYIKNQTE
                     DICLEAVRQNCNAIKCVQNQTEKICIEAVKQDWRMLEFVEEPTEEICMLAINQDGTAL
                     KYIENQTEELCLRAVEKNGAALEYVKEQTEEICIEAVRNSEFGLARVKNQTEKICMEA
                     VKHCSYNLKWVENQTEEICMEAVRQNGLDLKFVKNQTETICLRAVRQNGMALEFVKEQ
                     TVGICLKAVRQYGMALKFVKEQTEEICTEAIKQDKRALSFVKGDKEKYKALYDNNEPF
                     AKRYVRNVIEKENRALIKKGEENAKIKIAGKLYDRGMSFEDISDIVEIEISKLKVSLG
                     IF"
     gene            1323..1811
                     /locus_tag="KRL67_003642"
     CDS             1323..1811
                     /locus_tag="KRL67_003642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HBH1524477.1"
                     /translation="MKITVLYQTRSGNTERVAKLIEDGAKKVDGVDVKLMRLDNIDLD
                     YLNESKAVIFGTPTYLANTTWEVKKWFDEDSKKVNLAGKLGAVFATCDYICGGPDVAI
                     LTIVGHLMVKGMLVYSGGGSLGKPFIHLGHVHSIEGPELQDEKAIIFGERIATKAKEL
                     FA"
     gene            1987..3915
                     /locus_tag="KRL67_003643"
     CDS             1987..3915
                     /locus_tag="KRL67_003643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HBH1524478.1"
                     /translation="MVWHIEYEIFSAIIVIFLMVYFFRSKFIPTLQNKIYCALLIFSF
                     LFIISNILGSFCLNNIDKIPIFITFLLNQIYLLLLPIPAALVSFYVMAIIYQDIRYMK
                     KRLLFLSIPLIICIILSITNPFTHILFGLSKETGYIRGCGYVATFASFFFYAIYSAFL
                     SFKYRKAMHESKIWAIRGFLIVSIVAIILQAIFLQYLLTGTACACSLLLTYLSMQNRG
                     LIIDDLTGVLNRQSFIQELDLNINTEQSGFIITIALDDFKFMNETFGTKNGDIALKEV
                     GKYLIQIHDVNHVYRIGGDIFSIVVNSQLGIEPDDIIRKIEERFKKPWLVEEISFNLA
                     TSIAVVYYPENAETTEDVIVAIDFSIHEAKRSESRRVVYADASISEKIKKKHVIKNCL
                     KEALKNDGFEVYYQPIFSNNRGRFTSAEALLRLEHKELGFIPPDEFIPIAEQTGLINS
                     IGLVVFEKVCRFIASDEFENLKLDNIAVNLSVVQCMQKNLADDLLYLMNKYNVSPTKF
                     KLEITETVAMGSFNIIKETMERLIDLGVKFALDDFGIAYSGVTNMLSLPFSVIKLDKS
                     LIWSMNEDSRHKLTVETIIALINKLNMKAVAEGVETIKCAEDLIAMECEYLQGYYFSK
                     PVPEDVFKNLLKEKTFTS"
BASE COUNT         1559 a          389 c          731 g         1347 t
ORIGIN      
        1 tataccacat attttgggta ttgtatactg gaattagagt aattaattta ttttttaaca
       61 tacaaatgat tctgtgaaaa aaaacaagac tcaaaaattt ttatattctt tgatagaata
      121 aaattgagag taaatttatt gaattttata aaatttataa ggggtgagtc taagatatgc
      181 atgaagataa aaatgatata tttgaatcaa tagatactga ggattttgat aataaaacag
      241 aagaagaatg tttagagcta gtaagaaaaa atggattgaa tttaatatac ataaaaaatc
      301 aaacagaaga tatatgtcta gaggctgtta gacaaaattg taatgcaata aaatgtgttc
      361 agaatcagac agaaaaaata tgtatagaag cagttaaaca agattggaga atgttagaat
      421 ttgtggaaga accaacagaa gaaatttgca tgttggctat taatcaagat ggaacagcat
      481 taaaatatat agaaaatcag acagaggagt tatgcttaag agctgttgaa aaaaatggtg
      541 ctgctttgga gtatgtaaaa gaacaaacag aagaaatatg tatagaagca gttagaaaca
      601 gcgaatttgg attggcaaga gtgaaaaatc aaacagaaaa aatatgtatg gaagcagtta
      661 agcattgtag ctataatcta aaatgggtag aaaatcaaac agaagaaata tgtatggaag
      721 cagtgagaca aaatgggctt gatttgaaat ttgtaaaaaa tcaaactgaa acaatatgtt
      781 taagagcagt aagacaaaat ggaatggcat tagaatttgt aaaggaacag actgttggta
      841 tatgtttaaa ggcagtaaga caatatggaa tggcattaaa atttgtaaaa gaacaaacag
      901 aagaaatttg tacagaagct ataaaacaag ataaaagagc acttagtttt gtaaaaggag
      961 ataaggaaaa atacaaggct ctatatgata ataatgaacc atttgcaaag cgatatgtta
     1021 gaaatgtaat agaaaaagaa aacagagcat tgattaaaaa aggagaagaa aatgcaaaaa
     1081 tcaaaattgc aggaaaactt tatgatagag gcatgtcttt tgaagatata tcagatatag
     1141 ttgagattga aataagcaag ttaaaagtga gtttaggtat tttttaaagt aataaatgta
     1201 gacgtttttt atgggaaata gttaataaaa aaataatcga ctagctcttt gtaggacagc
     1261 aaaattagtg atataatttg atgtataata aattattttt attagataag ggggaattaa
     1321 agttgaagat tacagtatta taccaaacaa gaagtggaaa cacagaaagg gttgctaagc
     1381 tgatagaaga tggagctaaa aaggtggatg gagtagatgt aaagcttatg agacttgata
     1441 atattgattt agattattta aatgaaagta aagcagtaat atttggaaca cctacttatt
     1501 tagcaaatac aacttgggaa gtaaaaaaat ggtttgatga agattcaaaa aaagttaatt
     1561 tagctggaaa attaggagct gtttttgcta catgtgatta tatatgtgga ggtccagatg
     1621 ttgcgatact tactatagta ggacatttga tggttaaagg gatgcttgtg tattctggag
     1681 gtggttcttt aggaaagcca tttatacatt taggacatgt acattcaatt gaaggtccag
     1741 agttacaaga tgagaaggct ataatatttg gtgaaagaat tgctactaag gcaaaagaac
     1801 tatttgctta atttctatat gcataataat gattagagta aatttagggt tttaatggag
     1861 atttgaagta aaagttataa aataaaaaat tttccataaa attactcaag atgaattata
     1921 atataattat aaataaatta tattattaaa atatttaaaa tcaaacgtca atagcgaggg
     1981 aagtttatgg tatggcatat agaatatgag attttttctg caattatagt tattttttta
     2041 atggtatact tttttagaag taaatttata cctactttac aaaacaaaat atattgtgca
     2101 cttttaatat tctcattttt atttataatt tcaaatattt taggttcatt ttgtctaaat
     2161 aatatagaca aaattccaat ttttattact tttttattga atcagatata cttattgtta
     2221 ttgccaatac cagcagcact tgtatcattt tatgttatgg ctattattta tcaagatata
     2281 aggtatatga aaaaaagatt attattttta tctataccac ttataatttg tataatctta
     2341 tctattacaa atccttttac gcatattcta tttggattgt caaaagaaac aggttatata
     2401 cgaggttgtg gatatgttgc aacttttgca tcttttttct tttatgcgat atattcagca
     2461 tttttatctt ttaagtatag aaaagcaatg catgaatcaa aaatatgggc tattagaggt
     2521 tttcttatag tttcaattgt ggcaattata ttacaggcaa tttttttaca gtatcttctt
     2581 acaggaactg catgtgcatg tagtttactt ttgacatatc tttctatgca aaatcgaggt
     2641 cttattattg atgaccttac tggtgtactc aatagacaat cttttattca agagctagat
     2701 ttgaatatca atactgaaca atcaggattt attattacaa ttgctcttga tgattttaag
     2761 tttatgaatg aaacttttgg aacaaaaaat ggagatattg cactaaaaga agttggaaaa
     2821 tatttgattc agatacatga tgtaaaccat gtatatagaa tagggggaga tatattttct
     2881 attgttgtca atagtcaatt aggtatagaa ccagatgata ttatcagaaa gatagaagaa
     2941 agatttaaaa agccttggct agtagaagag ataagtttta atttagcaac tagtattgct
     3001 gttgtttatt acccagaaaa tgcagaaacg acagaagatg taattgttgc tatagatttc
     3061 tctatacatg aagcaaaacg ttctgagtca aggcgagttg tttatgcaga tgcgtctata
     3121 agtgaaaaga taaagaagaa acatgttatt aaaaattgtt taaaagaagc attaaaaaat
     3181 gatgggtttg aagtgtacta ccaacctata ttttctaata acaggggcag gtttacttca
     3241 gcagaggcat tacttaggct ggaacataaa gagttaggtt ttatacctcc tgatgaattt
     3301 attccaattg cagaacaaac aggattgatt aactcaattg gattagtagt ctttgagaag
     3361 gtgtgtagat ttatagctag tgatgagttt gaaaatttaa aattggataa tatagctgtg
     3421 aatctttctg tagtacaatg tatgcaaaaa aacttagctg atgatttact ttatcttatg
     3481 aataaatata atgtatcacc aactaaattt aaacttgaaa ttactgagac tgtggctatg
     3541 ggctctttta atataattaa agaaacaatg gaaagattaa ttgatttagg tgttaagttt
     3601 gcacttgatg attttggaat tgcttattct ggagttacta atatgctttc tttaccattt
     3661 tcagtaatta agttagataa gagtttgatt tggtctatga atgaagatag taggcataaa
     3721 cttacagttg agacgattat agctcttata aataaattga atatgaaggc tgtagcagaa
     3781 ggcgttgaaa ccattaagtg tgctgaagat ttaatagcta tggagtgtga atatttgcaa
     3841 gggtattatt tttctaaacc agttccagaa gatgttttta aaaatttgtt gaaagaaaag
     3901 acttttacaa gttagcaccc tttatttttt taattacaac tttgttcatt gacactgtca
     3961 gcgatatatt ataaaattaa cacaataata agtattaaat ttattataca tattattaat
     4021 attaat
//
LOCUS       DAEPXF010000259         3999 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain HB27
            SAMN13376389-rid12479423.denovo.267, whole genome shotgun sequence.
ACCESSION   DAEPXF010000259 DAEPXF010000000
VERSION     DAEPXF010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13376389
            Sequence Read Archive: SRR10518432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3999)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3999)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001069624.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001069624.1
            Genome Coverage       :: 315x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/15/2021 01:54:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,165
            CDSs (total)                      :: 4,076
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 89
            rRNAs                             :: 10, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 10 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 19 of 69
            Pseudo Genes (incomplete)         :: 44 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 10 of 69
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3999
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13376389-rid12479423.denovo.267"
                     /strain="HB27"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="China: Hebei"
                     /collection_date="2017"
     gene            complement(<1..1007)
                     /locus_tag="KRL67_003644"
     CDS             complement(<1..1007)
                     /locus_tag="KRL67_003644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HBH1524479.1"
                     /translation="MMGKIRFGIVGTSNIANIFLRAASRVKGFELVAVYSRNLEKARE
                     FGTPHGASVFFDDLEQMAKSKDIDAVYIASPNALHSRQAITCLKHKKHVLCEKSLASN
                     LKEVKSMIKTAKDNNVLLMEAMRITCVPNFRAVKENLYKIGKIRRFFGSYCQYSSRYD
                     KYKNGIIENAFKKELSNGALMDIGVYCIHPIVNLFGAPKTVKAVSHILQTGVDGEGSA
                     VFQYDDMDAIIQYSKIADSYIPSEIQGEDGSIIIEKLNLFEKAIIKYRDGREEDISIP
                     KEKAEEKPREIEGIYYELVEFISLINNNKIESNINSHENSIIVMEIMDEIRRQSSIVY
                     PV"
     gene            complement(1184..1723)
                     /locus_tag="KRL67_003645"
     CDS             complement(1184..1723)
                     /locus_tag="KRL67_003645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative ABC transporter permease"
                     /protein_id="HBH1524480.1"
                     /translation="MDNVGSLLYFLFNFFLYGFIGWIIENLYSYYKQGHFQEDGFLNG
                     PFKPMYAIAMSIIIEVSRFIDPNNIFALLLLCLVVPTVVEYITGILMKVYFNKVYWDY
                     SDYKYNSRGIVCLKFSVYWTILTFIGVRYFQVYIVDSLYSLIIPYWPIVVTILLVTLF
                     VDEILTIKFLRGNKDIVER"
     gene            complement(1824..2504)
                     /locus_tag="KRL67_003646"
     CDS             complement(1824..2504)
                     /locus_tag="KRL67_003646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HBH1524481.1"
                     /translation="MQKVEGIIFDMDGVLFDSERISLEFWMETFEKYGYTMTKEIYTS
                     VMGRNRKGIIEGLTNIYDSSVPIIDLYDEKTKNMIEFMERKGAPIKLGVNELISFLKE
                     NGYKMAVATSTKRERAVKRLAKANLKDCFDAIVCGDDVVNSKPNPEIFLKAAKKINVN
                     PKNCIVIEDSPMGVEAAYNGGIRCINVPDLKEPDEQIKSQSHKILENLLEVREYLKSL
                     NSKECHNL"
     gene            complement(2533..3822)
                     /locus_tag="KRL67_003647"
     CDS             complement(2533..3822)
                     /locus_tag="KRL67_003647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HBH1524482.1"
                     /translation="MRELLKVTLIVMLVLVNAFFVACEFAMVKLRSSRIDTMITEGNN
                     NAKRCKIIKDNLNSYLSACQLGITLCSLALGWMGESTVKELILPIVSVFNLSESIIYT
                     ISISISFLIITMMEVVIGELVPKALALYNTEKIMLSTSFLLVGFYKLTYPIIYFFNLS
                     TDLFLKPFGYSQADEVDEPHTGDEIRLLVEESYKSGLIDESEQRLVDNIFEFEEKKIR
                     EIMVPRTDMVCIYESDSEEKILAILKEEGVTRYPVCRKNKDDILGFVHIRDLYNQKIN
                     ENKIELEEILRDIIYISENLTIDKALERIRKEKLQLAIVVDEYGGTSGVVTIEDILEE
                     IVGEIQDEFDKEETHIKKIGVGSYLVDGTESINYINKYFGLDIEHDGFDSIGGWISYM
                     LGSNIKVNQSVTFENYNFTILELDKLRVVKLIIKSVI"
BASE COUNT         1330 a          724 c          372 g         1573 t
ORIGIN      
        1 actggataaa caatactact ttgtcttctt atctcatcca taatttccat tactattatt
       61 gaattttcat gagaatttat atttgattct attttattgt tatttataag acttataaac
      121 tctacaagtt cataatatat tccttctatt tctctaggct tttcctctgc tttttctttt
      181 ggaatagata tatcttcttc tcttccatct ctgtatttaa taatagcttt ttcaaataga
      241 ttaagctttt caattattat acttccatct tctccttgta tttcagatgg tatataagaa
      301 tcagctattt tagaatactg aattatcgca tccatatcat catattgaaa tacagctgaa
      361 ccttctccat ccactccagt ttgaagtata tgagaaactg cctttacagt ttttggagca
      421 ccaaataaat tcactatagg atgtatacaa tacactccta tatccattaa agctccattt
      481 gacaactctt ttttaaaggc attttctata attccatttt tatatttatc atatcttgaa
      541 gaatactgac aatagcttcc aaaaaatctt cttattttac ctattttata caagttttct
      601 ttaactgctc taaagtttgg aacacatgta attctcatag cttccataag caatacatta
      661 ttatctttgg ctgtttttat cattgatttg acttctttta aattagatgc taacgacttt
      721 tcacaaagca catgtttttt gtgttttaaa caagtaatgg cttgccttga atgtaatgca
      781 tttggagaag ctatatacac agcatctata tccttcgatt tagccatttg ttctaaatca
      841 tcaaaaaaaa cacttgctcc atgtggagtt ccaaattctc tagctttttc taaatttcta
      901 gaatatacag caaccaattc aaagccttta accctagatg cagctcttag aaatatattt
      961 gctatgttgc ttgtcccaac aataccaaat cttattttac ccatcataaa tcactcctcc
     1021 attttctaca ctttatttta attttaataa aaaactttac aacttataga tatattataa
     1081 aataatttta tcaaataaaa aagaaagcta attttaaatg attttaccat tttaacatag
     1141 ctttctttta actacaatct aaattcaact tatattttta aagttatctt tctactatat
     1201 ctttatttcc cctcaaaaat tttatagtaa gaatttcatc cacaaacaat gttactaaaa
     1261 gtatagttac aacaattggc caataaggta ttataagact atataaacta tcaactatat
     1321 acacttgaaa atatctaact ccaataaatg tcaatatagt ccaatataca gaaaacttta
     1381 gacatactat tcctctacta ttgtacttat aatctgaata atcccaatag accttattaa
     1441 aatatacttt cataagtatt cctgtaatat attctactac tgtcggcact actagacata
     1501 ataaaagcaa tgcaaatata ttatttgggt caataaatct tgagacctct attataatcg
     1561 acattgcaat tgcatacatt ggtttaaatg gaccatttaa aaatccatct tcttgaaaat
     1621 gcccttgttt ataataacta tataagtttt caattatcca acctataaaa ccatacaaaa
     1681 aaaaattaaa taaaaaatat aatagagaac ctacattatc catgaatatt cctcctagct
     1741 gtttatactt attttttctc atattcatgg atttattcta aatcaaagaa attatattta
     1801 atttatttaa ttaatatttt atattataga ttgtgacatt ctttagagtt taaacttttt
     1861 aggtattctc taacttccaa taaattttcg agtatcttat gtgattgtga tttaatttgc
     1921 tcatctggct cttttaaatc tggtacattt atacatctta taccaccatt gtatgcagct
     1981 tcgactccca taggtgaatc ctctatgaca atacagtttt tgggattaac atttatcttt
     2041 ttagctgctt ttaagaatat ctcagggttt ggtttagagt ttacaacatc atctccacaa
     2101 actatagcat caaagcaatc ttttaaattt gccttagcta atctctttac tgctctttct
     2161 ctctttgttg atgttgcaac agccatcttg tatccatttt cctttaaaaa agagattagt
     2221 tcattgactc ctaatttgat aggagctccc ttcctctcca taaattcaat catattttta
     2281 gttttttcgt catataaatc tattattggc acagaactat cgtatatatt tgtaagacct
     2341 tctattattc ctttacgatt tcttcccata actgatgtat aaatttcctt agtcatagta
     2401 tatccatact tttcaaacgt ctccatccaa aattcaagag aaattctctc tgagtcaaac
     2461 aaaactccat ccatgtcaaa aataattcct tctacttttt gcatacaatt cctccaatat
     2521 tttttgatat aattatatta cactttttat gattaatttt acaaccctca atttatccaa
     2581 ttctaatatt gtaaaattgt aattttcaaa agtgacgctt tggtttactt taatatttga
     2641 accaagcata taggatatcc acccacctat actgtcaaat ccatcatgct ctatatcaag
     2701 tccaaaatac ttattaatat agttaataga ttcagttcca tcaactaagt aagaacctac
     2761 tcctattttc ttaatatgag tctcttcttt gtcaaattca tcttgaattt ctccaactat
     2821 ctcttctagt atatcttcta ttgttaccac accagatgta ccaccatatt catccacaac
     2881 tatagcaagt tgaagttttt cctttcttat tctctcaaga gctttatcta tagttaaatt
     2941 ttcagatata tatataatat ccctcaatat ttcttctagt tcaattttat tttcatttat
     3001 cttttgattg tataaatctc ttatatgtac aaaacctaat atatcatctt tattttttct
     3061 gcatactgga tatcttgtaa caccttcctc ttttaaaata gctaatatct tttcttcaga
     3121 gtcactttca tatatacata ccatatcagt tctaggaacc attatctctc taattttctt
     3181 ttcttcaaat tcaaaaatat tatcaactaa tctttgctct gattcatcta taagtccact
     3241 tttataactt tcttctacta atagtcttat ttcgtcacct gtatggggtt catcaacctc
     3301 atcagcttgt gagtatccaa atggtttaag aaataaatca gtacttaagt tgaaaaaata
     3361 tattattgga tatgttaact tataaaaccc tactaataaa aaagatgtac tcaacattat
     3421 tttttctgta ttataaagtg ctaaagcttt aggaaccaac tctcctatta caacctccat
     3481 cattgttata attaaaaaag aaatggaaat tgatatagta tatataatac tttcactaag
     3541 attaaataca cttactattg gtaaaatcaa ctctttaact gtagattctc ccatccaacc
     3601 taatgcaagt gaacaaagcg ttataccaag ttggcatgca gaaagatatg aatttaaatt
     3661 gtcttttatt attttacatc tttttgcatt attattacct tcagttatca tggtatcaat
     3721 cctagaacta cgaagtttta ccatagcaaa ttcacaagct acaaaaaaag catttactaa
     3781 aacaagcatt actataagtg ttacttttaa taactcccgc ataaagtatt ggttcttata
     3841 atgtaagaaa tctataagaa ctcaaagttc cccccttata ttaataaatt taatttataa
     3901 tatatattct atcatattta ttaatatatc ctactttatt acaaataatt tttatgaact
     3961 taataacccc ttttaatatt tatgattggc tatttgata
//
LOCUS       DAEPXF010000260         3977 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain HB27
            SAMN13376389-rid12479423.denovo.268, whole genome shotgun sequence.
ACCESSION   DAEPXF010000260 DAEPXF010000000
VERSION     DAEPXF010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13376389
            Sequence Read Archive: SRR10518432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3977)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3977)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001069624.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001069624.1
            Genome Coverage       :: 315x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/15/2021 01:54:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,165
            CDSs (total)                      :: 4,076
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 89
            rRNAs                             :: 10, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 10 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 19 of 69
            Pseudo Genes (incomplete)         :: 44 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 10 of 69
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3977
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13376389-rid12479423.denovo.268"
                     /strain="HB27"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="China: Hebei"
                     /collection_date="2017"
     gene            complement(<1..879)
                     /locus_tag="KRL67_003648"
     CDS             complement(<1..879)
                     /locus_tag="KRL67_003648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cache domain-containing protein"
                     /protein_id="HBH1524483.1"
                     /translation="MTGRNKSFTLIIILFLTSLFLTMGYIYIEDTKHLLMSEAKIHIK
                     EVAIQGSQLAQRQIEKDLDVLNIFSNYYASNPDIPNEEKMKNLLDEMENQKFYTMAIV
                     DINGNAENTKGDKFSVKDREFFKNSIKGKKYVSSPYVDEVNKSIKKIAISVPLLNNDK
                     VVGVLYCTYNINTLMKLINVSFYENNSISYVVKNDGTIILHPQGDRLSKNIYKLLKQD
                     NDIQEVNRLKKELQENKTGATVLNMLEERRYLGYATMDNGNSENNYIKNWNLIFSIPE
                     TVVFSNSEQIINRAIYA"
     gene            complement(1150..3804)
                     /gene="adhE"
                     /locus_tag="KRL67_003649"
                     /gene_synonym="adhC"
     CDS             complement(1150..3804)
                     /gene="adhE"
                     /locus_tag="KRL67_003649"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HBH1524484.1"
                     /translation="MAHKEQVIIENKKENEVMSVGLETVNNVESLVKKLAKIREAQKI
                     FATYTQEQVDKIFLAASLAANKQRVPLAIMAQKETGMGIAEDKVIKNHYASEYIYNAY
                     KETRTCGVIEKDEAFGMTKIAEPIGVIAAVVPTTNPTSTAIFKALLALKTRNGIIFSP
                     HPRAKNSTIEAAKVVLEAAVLAGAPEGIIGWIDEPSLELTTTVMKEVDLTLATGGPGM
                     VKSAYSSGKPAIGVGAGNTPAIIDESADIKTAVNSILVSKTFDNGMICASEQSVIVLE
                     NIYNEVKKEFKERGAYLLDKDETEKIRNIILVNGGLNSKIVGQTACTIAKLAGFEVPV
                     DTKVLIGEVESVEIEEAFAHEKLSPVLAMYKASDFDDAVRKAEKLVEDGGFGHTSSLY
                     IDDVNQREKLDKFTSAMKTCRILINTPSSQGGIGDLYNFKLAPSLTLGCGSWGGNSVS
                     ENVGVKHLLNIKTVAERRENMLWFRAPEKVYFKKGCLGVALKELKDVMNKKRAFIVTD
                     TFLYNNGYTKAVTDLLDEMNIKHTTFFEVEPDPTLECAKIGAKAMREFNPDVIISIGG
                     GSAMDAGKIMWTLYEHPDVDFQDLAMRFMDIRKRVYTFPKMGEKANFVAIPTSAGTGS
                     EVTPFAVITDQDTGVKYPLADYELMPNMAIVDSDMMMNMPKSLTSASGIDALTHALEA
                     YVSMLATDYTNGLALQAIKSIFEYLPRAYDNGAKDPEAREKMANASTMAGMAFANAFL
                     GVCHSMAHKLGAFHHVPHGVANALLITEVMKFNSSDAPKKMGAFSQYKYPEALKRYAE
                     IASFLGLKGNSDEEKFQSLLVAIEDLKLKVGIPKSIKEFGVEEFKFMDSIDEMVIQAF
                     DDQCTGANPRYPLMNEIKDMYLNSYYGR"
BASE COUNT         1240 a          728 c          462 g         1547 t
ORIGIN      
        1 tgcatatata gctctgttga ttatttgttc tgaattacta aatacaacag tttctggaat
       61 tgaaaaaatc aaattccagt tttttatata attattttca ctattcccat tatccatagt
      121 agcatatcct aaatatcttc tttcttccaa catatttagt acggttgcac ctgttttatt
      181 ttcttgcaat tcttttttta atctatttac ttcttgaata tcattatcct gttttaacaa
      241 tttataaata ttttttgata gtctatcccc ttgtggatga aggataattg taccatcatt
      301 ttttacaaca taagatatac tattattttc ataaaaagat acattaataa gtttcatcag
      361 agtattgata ttgtaagtac aatataaaac tccaactact ttatcattat ttaataatgg
      421 tactgaaata gcaatcttct ttatagactt gtttacttca tcaacatatg gacttgaaac
      481 atacttctta cctttaattg agtttttgaa aaattctcta tctttcactg aaaacttatc
      541 acctttggta ttttcagcat taccatttat atcaactatt gccatagtgt aaaatttttg
      601 attttccatt tcatctaaaa gatttttcat tttttcctca ttaggtatat ctggatttga
      661 agcataatag ttagaaaaaa tatttaaaac atctaaatct ttttcaattt gtctttgtgc
      721 tagttgagag ccttgtattg caacttcttt tatatgtatt tttgcctctg acataagaag
      781 atgttttgta tcttctatat agatataccc cattgtcaaa aataatgatg tcaaaaaaag
      841 aattataatt aatgtaaaac ttttatttct ccctgtcatt cgactcctcc cctaattagt
      901 caatacttca tgtagataat atatcctttt tataccaatt catcatttat attgtatata
      961 aatattctta aatgttacac ataaaatcaa cctaataacc ataaaaacat tcttttatta
     1021 ttataataac actatttaat tgaattttaa attaaagaat aaaaactatc cattttctca
     1081 aacaaaaaaa ctcttataca ataaaacttt gttttatgta taagagtttt tatctatttt
     1141 atagtaaact tatcttccat aatatgaatt taaatacata tcttttattt cattcataag
     1201 tggatatctt ggatttgctc ctgtacattg gtcatcaaat gcttgaatta ccatttcatc
     1261 tattgaatcc ataaacttaa attcttcaac tccaaactct tttatagatt taggaatacc
     1321 aactttaagt tttaaatctt caatagctac caataaactt tggaatttct cttcatcaga
     1381 atttcccttt aatcctaaaa atgaagctat ttcagcatat ctctttaatg cttctggata
     1441 tttatattga gagaatgctc ccattttctt tggtgcatct gaagaattaa atttcataac
     1501 ttctgttatc aaaagagcat ttgcaacacc atgtggtaca tggtggaaag cacctaattt
     1561 atgagccata gagtgacaaa cacccaaaaa tgcatttgca aaagccatac ctgccattgt
     1621 agaagcattt gccattttct ctctagcttc tgggtctttt gcaccattat catatgcacg
     1681 tggaagatat tcaaatatac tcttaatagc ttgtaaagct aatccatttg tataatcagt
     1741 tgcaagcata gatacatatg cttctagtgc atgagttaaa gcatctatac cagaagcaga
     1801 tgttaagctt tttggcatat tcatcatcat atcagaatca actatagcca tattcggcat
     1861 cagttcataa tctgctaatg gatattttac tcctgtatct tggtcagtta taacagcaaa
     1921 tggtgttact tctgaaccag tacctgctga tgttggtata gctacaaaat tagctttttc
     1981 acccatttta gggaatgtat ataccctttt tcttatatcc ataaatctca tagctaggtc
     2041 ttggaaatct acatctggat gctcatacaa tgtccacatt atctttcctg catccattgc
     2101 acttcctcca cctatagata ttattacatc tggattaaat tctctcatag ctttagcacc
     2161 tatcttagca cattccaaag ttgggtctgg ttctacttca aagaatgtag tgtgcttaat
     2221 attcatttca tctaataaat cagtaactgc tttagtatat ccattattgt aaaggaatgt
     2281 atctgttaca ataaaagctc tcttcttatt cattacatct ttgagttctt ttaatgctac
     2341 tcctaaacaa ccttttttga aataaacttt ttctggtgct ctaaaccaaa gcatattttc
     2401 tctcctctca gctactgtct taatattaag taaatgctta actcctacat tttcagatac
     2461 tgaatttcca ccccaagacc cacatcctaa agtaagagaa ggagctagtt taaaattata
     2521 taagtcccct ataccacctt gagaagaagg agtattaatt aaaattctac aagttttcat
     2581 ggctgaagta aacttatcaa gtttttctct ttgatttaca tcatcaatat ataatgaaga
     2641 tgtatgccca aatccaccat cttctactaa tttttctgct tttctaactg catcatcaaa
     2701 atcacttgcc ttgtacattg caagtactgg tgaaagtttc tcatgagcaa aagcttcttc
     2761 tatttcaact gattctactt cacctattaa aaccttagta tctactggga cttcaaagcc
     2821 tgcaagtttt gctatagtac aagctgtttg cccaactatt ttagaattta atcctccatt
     2881 tactaaaatt atatttctta ttttttctgt ttcatcttta tctaataaat aagcaccacg
     2941 ctctttaaat tcttttttga cttcattgta tatattttct aatactataa ccgattgttc
     3001 agaagcacat atcataccat tgtcaaaagt cttagataca agaattgagt ttacagcagt
     3061 ttttatatct gcactttcat caattattgc tggagtatta cctgcaccaa ctccaatagc
     3121 tggttttcca gatgaatatg ctgatttaac cattccaggg cctcctgtag caagggtcaa
     3181 atccacttct ttcataacag ttgtagttaa ttctaaagat ggctcatcta tccatcctat
     3241 tataccttca ggagctccag ctaaaacagc agcctcaaga acaacttttg cagcctctat
     3301 agtagaattt tttgctcttg gatgtggaga gaaaattatt ccatttctag tttttaaagc
     3361 taaaagtgct ttaaagatag cagtagaagt tggattagtt gtaggtacaa ctgctgcaat
     3421 aacacctatt ggctcagcaa ttttagtcat accaaatgct tcgtctttct ctataacacc
     3481 acaagttcta gtttctttat acgcattata tatatactct gaggcataat gattctttat
     3541 aaccttatct tcagcaattc ccataccagt ttctttttgt gccattatag ctagaggaac
     3601 tctttgctta ttagctgcca aagatgctgc taaaaaaatt ttatctacct gctcttgagt
     3661 atatgtagca aatatttttt gagcttctct gatttttgct agttttttaa ctaagctttc
     3721 aacgttattt acagtctcta aaccaactga cataacttca ttttcttttt tattttctat
     3781 tattacttgt tctttatgag ccatcttaac aacctcctga gatagttttt tatttattat
     3841 gttaatttat taacattata gcatttactt tttctatttt caagttattt ttttaacatt
     3901 ttatttttta tctaatataa catcatttat accaataata tctagcttta caaatgctta
     3961 gtttaactag ctatcac
//
LOCUS       DAEPXF010000261         3964 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain HB27
            SAMN13376389-rid12479423.denovo.269, whole genome shotgun sequence.
ACCESSION   DAEPXF010000261 DAEPXF010000000
VERSION     DAEPXF010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13376389
            Sequence Read Archive: SRR10518432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3964)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3964)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001069624.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001069624.1
            Genome Coverage       :: 315x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/15/2021 01:54:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,165
            CDSs (total)                      :: 4,076
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 89
            rRNAs                             :: 10, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 10 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 19 of 69
            Pseudo Genes (incomplete)         :: 44 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 10 of 69
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3964
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13376389-rid12479423.denovo.269"
                     /strain="HB27"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="China: Hebei"
                     /collection_date="2017"
     gene            179..1033
                     /locus_tag="KRL67_003650"
     CDS             179..1033
                     /locus_tag="KRL67_003650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HBH1524485.1"
                     /translation="MEWIERLNKAINYIEENITKEIEYEQVAKIACCSTYHFQRMFAY
                     MADVPLSEYIRRRRMSLAAVELQNDDKKIIDVALKYGYSSPTAFNRAFKSIHGVAPSF
                     IKKGEITTLKAFPPISFKINIKGAEEMNYRIEKKEAFRIVGVSQPLHNELEKNFEIVP
                     QMWQKVALDGTLQKLIPMMDSQPQGVLGISICNNSEKWKYFISVSSTKSIDDTLEEYT
                     VPAFTWAIFSGEGQCPQAMQELEKRIVTEWLPTSGYEYDNGPDIELYLNPDPQNAKFE
                     VWIPVVKK"
     gene            1233..2738
                     /locus_tag="KRL67_003651"
     CDS             1233..2738
                     /locus_tag="KRL67_003651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HBH1524486.1"
                     /translation="MNVAIYLRKSRADEEAEHRGEFETLSRHKTTLLKLAREQNLNVI
                     EIKEELVSGESIEYRPKMIELLDEIKNNNYDAVLVMDIDRLGRGNMQDQGLILDTFKK
                     SKTKIITPRKTYNLNDEFDEEYSEFEAFMARKELKLISRRMQRGKIKSIEEGKFIAQA
                     PPYGYDIKYLNNKERVLVINEDKATVVKIIFDMYLNGDGACKISNYLNELGYKTTTGR
                     TWYEKGIRDIIKNKVYCGYIVWNKVERKRNSSRTRGIEEQIEVKGKHEPIISEDIWNK
                     AQNIRKKRINSPTPNNKITNPLAGIVKCGYCNHTLIAKSNTTSSGYEKYMVCTNCKNK
                     ATKLYILENKILEVLNKWLVNYRLSLKDEKDNSKPENTLEHTLINLNKELDTLEKQKE
                     RLHDFLEQGIYDSNTFGERLKILLSRIENVREAINKTNIDIEMQKERNTVKTQIVPQL
                     EKILSEYTEEKEPYLKNVLLKSVINYAVYKKEPNQKSGDFSLVLYPNIPRN"
     gene            2765..3265
                     /locus_tag="KRL67_003652"
                     /pseudo
     CDS             2765..3265
                     /locus_tag="KRL67_003652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897038.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
     gene            complement(3425..3772)
                     /locus_tag="KRL67_003653"
     CDS             complement(3425..3772)
                     /locus_tag="KRL67_003653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HBH1524487.1"
                     /translation="MHENKKNLLDGSLAQYNDAAVPKIPVFAGNDITSEVYYFKPNQV
                     LNAHRHPNGEQIFVFLKGEGKMKLGENECDVKNGDTVFVPTGEWHEITNGSNEEMIAV
                     QITKINAGAEYRG"
BASE COUNT         1604 a          488 c          617 g         1255 t
ORIGIN      
        1 aattagatac taatttgtga tatactttaa tgtgaaaaac aatactattt gttcattttt
       61 gtagttaatt acagactttc tcatgttcat tctttattaa gctagttaat tagtgcatag
      121 aaagagagga ttaactattt caaaatagat attatataaa ttacaaggag ctgattttat
      181 ggaatggatt gaaagattaa acaaagcaat taactatatt gaagaaaaca taacaaaaga
      241 aattgaatat gaacaagtgg caaaaatagc ttgttgttct acatatcatt ttcaaagaat
      301 gtttgcttat atggcagatg taccactttc tgaatatatt cgtcgtagac ggatgtcttt
      361 agcagctgta gaattacaaa atgatgataa aaagataatt gatgtggctt taaaatatgg
      421 atactcttct ccaacagcat tcaaccgtgc atttaaaagt attcatggtg ttgccccatc
      481 atttataaaa aaaggagaaa taacaacttt aaaagccttt cctccaatca gctttaaaat
      541 taatattaaa ggagccgaag aaatgaatta tagaattgaa aagaaagaag catttcgtat
      601 agttggtgta tctcagccac ttcacaatga actagaaaag aattttgaaa ttgttcccca
      661 aatgtggcaa aaagttgctt tagatggaac acttcaaaaa ttaatcccta tgatggatag
      721 tcaacctcaa ggtgtattag gtataagtat ttgtaataat tcagaaaaat ggaagtattt
      781 catctctgtt tcaagcacaa aatctataga tgatacatta gaagaatata ctgttccagc
      841 ttttacttgg gcaatatttt ctggagaagg acaatgtcca caagcaatgc aagaactaga
      901 aaagcgtatt gtaacagaat ggttacctac ttctggttat gaatatgata atggtcctga
      961 tattgaactg tacttaaatc cagaccctca aaatgctaaa tttgaagtat ggatacctgt
     1021 agtaaaaaaa tagtttaaaa ttaaaaaatg aagaaagcat aagattaata gtataaatat
     1081 ttacctagat tacaagcttt taaatatact ataaaattta ctgtataaaa ataaacctaa
     1141 aaaatatatc ttgtattttg aaggtttatt tttgtgtttt aaatataata aatctgttac
     1201 tgaatataat tatattcaag gggtgtgatt taatgaatgt ggcaatttat ttacgcaaaa
     1261 gtagagctga tgaagaagca gaacatagag gtgaatttga aactctaagt agacataaga
     1321 caactctttt aaaactagca agagaacaaa atctaaatgt cattgaaatt aaagaagaac
     1381 ttgtttctgg ggaaagtata gaatatagac ctaagatgat agagctacta gatgagatta
     1441 aaaataataa ctatgatgct gttttagtaa tggatattga ccgtttgggt cgtggaaata
     1501 tgcaagacca agggctaata ctagatacat ttaaaaaatc taagacaaaa attattactc
     1561 ctagaaaaac gtataactta aatgatgagt ttgatgagga gtattctgaa tttgaagctt
     1621 ttatggctcg taaggaatta aaattaatat ctagacgtat gcaaagagga aaaatcaaaa
     1681 gtattgaaga aggtaaattt atagctcaag ctcctcctta tggctatgat attaaatatt
     1741 taaataataa agaaagagta cttgtaataa atgaagataa agcaactgtt gttaaaataa
     1801 tctttgatat gtatttaaat ggagatggtg catgcaaaat atccaattat ttaaatgaac
     1861 ttggctataa gaccacaaca ggtagaactt ggtatgaaaa gggtataaga gatattataa
     1921 aaaacaaagt gtattgtgga tatatagtat ggaataaagt cgagaggaaa agaaattcta
     1981 gtagaacaag aggaattgag gaacaaattg aggtaaaagg aaagcatgaa ccaataataa
     2041 gtgaagacat atggaataag gcacaaaata taagaaaaaa acgtattaat tcccctactc
     2101 caaacaataa aataacaaat ccattggctg gaattgtaaa atgtggatac tgtaatcata
     2161 ctttaatagc caaatctaat actacatcaa gtggatatga gaaatatatg gtttgtacta
     2221 attgtaaaaa taaagctact aaactataca tattagaaaa taaaatatta gaggtcttaa
     2281 ataaatggtt agtaaattat agactatctt taaaagatga aaaagacaac tctaaacctg
     2341 aaaatacttt agaacatacc ttgataaatt taaataaaga attggatact ttagaaaaac
     2401 aaaaagaacg actacatgat tttttagaac aaggaattta tgacagtaat acatttgggg
     2461 aacgtttaaa aatactcttg tctcgtatag aaaatgtaag agaagctatt aataaaacaa
     2521 atatagatat tgagatgcaa aaggaaagaa atacagttaa aacacaaatt gttcctcaac
     2581 ttgaaaaaat tttaagtgaa tatacagagg agaaagaacc ttatctaaaa aacgtattgc
     2641 ttaaaagtgt aataaattat gcagtctata aaaaagaacc taatcaaaaa tctggtgatt
     2701 tttctttagt actttaccct aatatcccaa gaaattaatt atatatttta aatgttataa
     2761 atagactgat taccaatttg gtgtaatggc tggagctttt gcaggtggag agggtgactt
     2821 tacaacagca tttgaaccta cagcaacagc aatggaaaaa gaaggtagtg gatatatagt
     2881 cacatctata ggaaaagatt ctggagaaat accttttaca gcatactcta ctacgagatc
     2941 atacatgaaa aacaataaag atttaattca aagatttaca aatgctgttt ataaaggtca
     3001 attatgggtt caacaagcat ctagcaaaga agtagctaag gctatacaac ctttctttga
     3061 agatatatct ttagaagatt taacaacagt tgtagataga tataaatcaa tagatgcgtg
     3121 gtctcaaaac cctgtattaa aagaagaagg tttgaagaaa ttgatggctg ttatgaaaga
     3181 agctaaagaa ctagataagg aagcacctta tgaagatatt gtaaatactg agtttgcaaa
     3241 caatgctata aaaaatataa aataaaaatg tgcttaattt taaccaaata tttttctgct
     3301 tttatgataa aaattaataa atttataaaa atataaaaag agtcttttaa aagactcttt
     3361 ttatatttga gattaaagtt tatataataa aatagaaatc aaagtactag ttgtacctat
     3421 atatctatcc tctgtattct gctccagcat taatttttgt aatttgaaca gctatcattt
     3481 cttcattact tccattagta atttcatgcc actctcctgt tggcacaaat acagtatccc
     3541 cgttttttac atcacattca ttctcaccaa gtttcatttt accttctcct tttaggaaca
     3601 caaatatttg ttcaccatta gggtgtctat gagcattaag aacttgatta ggtttaaaat
     3661 aataaacttc tgaagttata tcgttaccag caaatactgg tatttttgga actgctgcat
     3721 cattatattg agctaagctt ccgtctaata gattcttttt attttcatgc atactagaaa
     3781 tacctctttt ctatattata gatttactat tattatacca atataagaaa atagtattct
     3841 cactttacac aataggaata aaattctttt atttttaaat acaagtatag tactattttt
     3901 atagttagaa aaatataatc cttataagat ataaaaaatt aacataatat aataaattct
     3961 aaaa
//
LOCUS       DAEPXF010000262         3944 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain HB27
            SAMN13376389-rid12479423.denovo.270, whole genome shotgun sequence.
ACCESSION   DAEPXF010000262 DAEPXF010000000
VERSION     DAEPXF010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13376389
            Sequence Read Archive: SRR10518432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3944)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3944)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001069624.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001069624.1
            Genome Coverage       :: 315x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/15/2021 01:54:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,165
            CDSs (total)                      :: 4,076
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 89
            rRNAs                             :: 10, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 10 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 19 of 69
            Pseudo Genes (incomplete)         :: 44 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 10 of 69
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3944
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13376389-rid12479423.denovo.270"
                     /strain="HB27"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="China: Hebei"
                     /collection_date="2017"
     gene            71..376
                     /locus_tag="KRL67_003654"
     CDS             71..376
                     /locus_tag="KRL67_003654"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH1524488.1"
                     /translation="MNNGFYKLNSVVLNFDIKNDVNKIRFNHYIMRKENIQRLQGTLP
                     NGQFYMTVRRAATDLQLSISTISRLVKEFLDLGIIRLVSKGVKGNRSVYSYVSIEFN"
     gene            401..1309
                     /locus_tag="KRL67_003655"
     CDS             401..1309
                     /locus_tag="KRL67_003655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conserved phage C-terminal domain-containing
                     protein"
                     /protein_id="HBH1524489.1"
                     /translation="MELDCVCNDEFIDNSGIEFDCNSKDNSFNGDINVETEHCINEYL
                     YDDDVEHNYNNFHNEDTIKNKAISNIRTIKKYIDESFDTNKSENSNLQQESINFNFND
                     TCNEIDKRTNNSIENNQNKRSMRLSDMNVLSDKGKTNKKNYFNHNHGIQKGTKSVTKK
                     KESLNKFNKKNIQQSIINMLNKKSGKNFKIDSPISIKLINERLKEGYVLEDFYKVIEV
                     KVAKWKNTIMEMYIRPETLFSYKFESYVNENISMKSQSNVHPCKESNSNDSSNYIHSD
                     YTTYDGSVRERKKYENVRYWNPICEI"
     gene            1708..3009
                     /locus_tag="KRL67_003656"
     CDS             1708..3009
                     /locus_tag="KRL67_003656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBH1524490.1"
                     /translation="MGKSKDVEIEKSILGTILLDNKLSYKLDELNENMFMNDICLEIF
                     KIMKELKKENIVIDVATVKSKIDKKSLAIKTSDVTNIITWGQNFGLDGHIKILKENLA
                     RRSINQNCQNLLHSLNLGENIDTCIYKFESNIKEILDKDTYENDDVNSIAGKVLDFLE
                     NKKDIGFKFGIKLLDTTIGGLFKGELTTIAAKSGVGKTALALQIMLNSFKQGKKTLFI
                     SREMTSEQVFMRNICRVTGVSTRNMKSKEIDENDWKLIVNAIGDLSENNLIYINDKID
                     TISAIRKRIRQVKPDLLIVDYVQLLTSQKSMDKREREVATFSRELKNMTLDFNIPVIQ
                     LSQLNDEMKDSRPYGDRPMRDSKAIYHDSNNVVYIHQLKGSDYEEAVRDIGESEEAVR
                     ASEYRGIKMVDLIVAKCRDGQTRHKHFCYFGDKLHFQELNY"
     gene            3085..3360
                     /locus_tag="KRL67_003657"
     CDS             3085..3360
                     /locus_tag="KRL67_003657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HBH1524491.1"
                     /translation="MLELTKIIELLEEHKIEVSIPKELHKYSLLEYLELCQQSGGTYF
                     YIKTLEGALKEARNELIIKLFNGKNYMELARMFEMSVINVRHIVRKK"
BASE COUNT         1650 a          328 c          735 g         1231 t
ORIGIN      
        1 tattttgtta tttatataaa aatacttatc tagttactag caattattag tagaaatggg
       61 ggaaatagag atgaataatg gattttacaa attaaattca gtggtattga actttgatat
      121 taagaatgat gttaacaaga taagatttaa tcattatata atgagaaaag aaaacataca
      181 aagacttcaa ggaactctgc caaacggaca attttatatg acagtgagaa gagctgctac
      241 agatttacaa ctttctattt ctactataag taggcttgta aaagagtttt tggatttagg
      301 gataataaga ttagtgtcta agggtgtaaa aggtaatcgt tctgtatata gttatgtaag
      361 tatagagttt aattgaagtt tatttgatga tagcaaatat atggaacttg actgtgtatg
      421 taatgatgaa tttattgata atagtggcat agagtttgat tgtaatagca aagataattc
      481 ttttaatgga gatataaatg tagaaactga gcattgcatt aatgagtatt tgtatgatga
      541 tgatgtagag cataattata ataatttcca caacgaagat actataaaga ataaagccat
      601 ttctaatatt agaactatca aaaaatatat tgatgaatct tttgatacaa ataagtcaga
      661 aaattctaac ttacaacagg agtcaataaa tttcaacttt aatgatacat gcaatgaaat
      721 agataaaaga acgaataata gtatagaaaa taatcaaaat aaaaggtcta tgagattgag
      781 tgatatgaat gttttaagtg ataaaggtaa gacaaataaa aagaattact ttaatcataa
      841 tcatggtata caaaaaggga ctaaatctgt aacaaagaaa aaagaatcct taaataaatt
      901 taataaaaaa aatatacagc aaagcattat aaatatgcta aataaaaaat caggtaagaa
      961 ttttaaaatc gattctccaa taagcataaa acttatcaat gaaaggctga aggaaggcta
     1021 tgtcttagaa gatttctaca aagttattga ggtaaaagtg gctaagtgga aaaataccat
     1081 aatggaaatg tatataagac cagaaacttt gtttagctat aagtttgaat cctatgtaaa
     1141 tgaaaacata tctatgaaaa gtcaaagtaa tgttcacccg tgtaaagaaa gcaatagtaa
     1201 tgatagcagt aattatatac attctgatta tacaacatat gatggaagcg tgcgtgagag
     1261 aaaaaaatat gaaaatgtaa gatactggaa tcctatatgt gaaatttaat tagtttaaga
     1321 atgtatttat gataaataag atattaatag agcatagtta gctatagcaa atgatatatc
     1381 aataattagt agcgaattat attgaacaat atatcaataa ttggtagtaa gttatattaa
     1441 gtaagatatt actatcgagt agtgaattat attgaacaat atattaataa tttgtagtaa
     1501 agtatatcaa ataagatatt gctattatgt agtaaattat ataaaacaat gtatcagaat
     1561 aaatagtata ttaataattt gtagtatatc atgacgaata atatagtcat aacaaatagt
     1621 gagtcataac aaataatgta tttataattg ataatgtatt taaaaatcaa aatataaaaa
     1681 cttatgagga aagtgagatt aatttatatg ggtaaatcaa aagatgttga aatagagaag
     1741 tctattttag gcacaattct tttagacaat aaattaagct ataagctaga tgaattaaat
     1801 gaaaacatgt ttatgaatga tatatgtctt gagatattta aaattatgaa agaacttaaa
     1861 aaagaaaaca tagtgattga tgtggccact gtgaaatcaa agatagataa aaaatctcta
     1921 gcaataaaaa cgagtgatgt gactaatata attacttggg gtcagaattt tgggcttgat
     1981 ggtcatatta aaattttaaa agaaaattta gctagaagaa gcataaatca aaactgccaa
     2041 aaccttttac acagcctaaa ccttggagaa aatatcgata cttgtattta caaatttgaa
     2101 agtaatataa aggagatttt ggacaaggat acttatgaaa atgatgatgt aaatagtata
     2161 gcagggaagg tgcttgattt tttagaaaac aaaaaagata ttggatttaa atttggtata
     2221 aaattacttg ataccactat aggaggtctt tttaaaggag agctaaccac aatagcagct
     2281 aaatcggggg ttgggaagac tgctttggca cttcaaataa tgttaaattc atttaagcaa
     2341 ggtaaaaaaa ctttatttat aagtagagaa atgaccagtg agcaagtatt tatgagaaat
     2401 atatgcagag taactggtgt aagtacaagg aatatgaaat ctaaagaaat agatgaaaat
     2461 gattggaaac ttattgtaaa tgcgataggt gatttaagcg aaaataactt aatttatatc
     2521 aatgataaga tagatacaat tagtgcaatt agaaagagga taaggcaggt aaagccagat
     2581 ttattaatag tggattatgt gcaactcctt acatctcaga aaagcatgga taagagagaa
     2641 agggaggtag ctactttttc gagagagctt aaaaatatga ctttagattt taatatacct
     2701 gtaattcaat taagtcagtt aaatgatgag atgaaagatt caagacctta tggagataga
     2761 cctatgaggg atagcaaagc catctaccac gatagcaaca atgtggttta tatacatcaa
     2821 ttaaagggga gtgattatga agaagcagtg agagatattg gagaaagtga ggaggcagtt
     2881 agagcatcag aatacagggg aattaagatg gttgatttaa tagttgcgaa gtgtagagat
     2941 ggacagacta gacataagca tttttgttac tttggggata agttacattt tcaagaacta
     3001 aattattagg tcaataaagt agtaataaca agcttattaa aagtcgagaa atagcaaata
     3061 gattataaaa tatgaggtga aaatatgttg gaattaacaa aaattataga attacttgaa
     3121 gaacacaaga tagaagtaag tattccaaag gagttacata agtatagttt attggaatac
     3181 cttgaattgt gtcaacaaag tgggggaaca tatttttata ttaagacact ggaaggggct
     3241 ttaaaagagg ctagaaatga gcttattatt aaattgttta atgggaaaaa ttatatggaa
     3301 ttagcgagga tgtttgaaat gagtgttatt aatgtcaggc atattgttag aaaaaaatag
     3361 atatttttgg agaatacaag tgtaatatac ttctgaaata gaagttatta taattgaaat
     3421 aaaattgtgg ttaatagatt aaagttgtgt ataagtttaa gattatagag gagtataagt
     3481 gtgattaaca gcataatttt gtgaataaaa ttgaggatat atatgagcta aatgtgtgat
     3541 taataaggta aggttgtgta taagattatg agtatgtaaa aggatagtct ataattaata
     3601 gtgcagagtt gtgtgtttgg ttaagattat ataagaattt aatagattaa actatactaa
     3661 taaggtaaaa tcgtgtgttt agttaaaact tattataaaa tcttgataca tgattaatga
     3721 tgcaatatgt gaataaagtt aagattataa tagatttaac ctgtgactaa tagaataaaa
     3781 ttgtgaataa agttaagatt ataataggtt taaattgtga ctaatagagt aagagtgtaa
     3841 atagaaataa acttcaatat gggcttaact tgtgactaac agagtagggt gtaaatagaa
     3901 ataaacttca atatgggctt aacttgtgat taatagagta gggt
//
LOCUS       DAEPXF010000263         3943 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain HB27
            SAMN13376389-rid12479423.denovo.271, whole genome shotgun sequence.
ACCESSION   DAEPXF010000263 DAEPXF010000000
VERSION     DAEPXF010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13376389
            Sequence Read Archive: SRR10518432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3943)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3943)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001069624.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001069624.1
            Genome Coverage       :: 315x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/15/2021 01:54:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,165
            CDSs (total)                      :: 4,076
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 89
            rRNAs                             :: 10, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 10 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 19 of 69
            Pseudo Genes (incomplete)         :: 44 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 10 of 69
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3943
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13376389-rid12479423.denovo.271"
                     /strain="HB27"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="China: Hebei"
                     /collection_date="2017"
     gene            411..875
                     /locus_tag="KRL67_003658"
     CDS             411..875
                     /locus_tag="KRL67_003658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBH1524492.1"
                     /translation="MNLNKVKFTEEHAIEVINWKYEGKYSIYNLPPWEEIKKKNFSLS
                     KEDKRKNFISFINDNKELIGFINLLDEGSSVFFGIGIKPEYCGMGIGKEIISLGLQEC
                     RNKYSTKPIVLNVRIWNMRAVKCYESQGFKIVETKVQKTHLGDGEFFVMRYQ"
     gene            complement(1142..1786)
                     /locus_tag="KRL67_003659"
     CDS             complement(1142..1786)
                     /locus_tag="KRL67_003659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBH1524493.1"
                     /translation="MFGQSFQNCLVLIKENFKLSTLTNLICSIILLCMIPLIQGVANL
                     DRISTAICLENYVAIIGMVMLVPVFSLEQSKEIDEIVSSKSLSQVIVQFIRILMSIIG
                     IVVLIIGFVNVLIYLGCNFPVVEYITGSFASAMFIGSLGMFFSNLFNNTIIGYMISIG
                     YLILNMMTKGKYVGKFFIMSMSQGSFDEKYWLILGSVVLIIASLLIKPIKRKIR"
     gene            complement(1716..3005)
                     /locus_tag="KRL67_003660"
     CDS             complement(1716..3005)
                     /locus_tag="KRL67_003660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBH1524494.1"
                     /translation="MNLFIKECKNILKSMVYYIFIVVLVFFYISQLGSSVSNDVSKNI
                     PNDGNPLVAPQKVENIEEYYSVHNQYPYGQKESMKTEKVIPNATINLIREYQENTYTT
                     YPTGVAKYIKLDKNKTKTIEKYIEEITSKSISEINLIWKTGESIKIDSSKVSFERFEE
                     IMQRVDDILGGGSAYAFDKLQRFGVVPVTYNEAMQNYNYTIQKDKVSNGYARIFCDYI
                     GIVLSIFPVFVAVFMSLKDKRSRMREIIYSKQVSSCKIVLSRYFALVFMMILPVLILG
                     MKDLLPLIFFAKDKGISIDNLAFIKYTLWWILPTLMIVTAVGMSITILTDTPVAIIVQ
                     LIWWFYSINSTGLKGDYSTMGLMIRHNSVLNGDIIIQNFNQIMINRIVISLGALVLVL
                     LAIFLYEQKRRGNLDVWSKFSKLFSSNKRKLQIKHFN"
     gene            complement(3002..3874)
                     /locus_tag="KRL67_003661"
     CDS             complement(3002..3874)
                     /locus_tag="KRL67_003661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBH1524495.1"
                     /translation="MEMKIAVSNLSKRFGKKQALDNINLEIDSGMFGLLGRNGAGKTT
                     FMRILATLLSKTEGSIHMCGVPIENTKEIRNMVGYLPQDFSMYPNMTVYKAMDYLAVL
                     SDIPLRKRRKIIAKLLSKVNLTNYQNVKVKSLSGGMNRRLGIAQALIHNPRVLIVDEP
                     TAGLDPEERIRFRNLLCEIANDRIVILSTHIVGDIEATCENVAILDSGKVIYNGSTEA
                     LAKLAEGKVYSIEVDKKDIENIKSRFIVIGMLTHGGKATLRIISDDKPFETAVNCNPT
                     IEDGYMLIMGGDNI"
BASE COUNT         1398 a          599 c          468 g         1478 t
ORIGIN      
        1 agttattgta tctctaaagt ttaagcatgg atagatatac atttgataat ttgcctataa
       61 tttacctact acttggagta ttggagcaaa tacaatatat taataataag ttcctaaaac
      121 caataaatta actaatacac ataaagatat tgatgttgat tatacaacta taagaaacgg
      181 atttaacaga atttaatcaa gctaagaaat tacatatatc gtgaggtttt taaattagaa
      241 ggtaattcaa aattagttta tagtcttctg tgaatccaat aaaaagtaca gggtacaaga
      301 attgttagta aaatttttaa agattttatg gataaatata aatagtttta cttattgtct
      361 aaaactttaa aataccatta attaccttaa acattttaat aggaggacat atgaatttaa
      421 ataaagttaa atttactgaa gaacatgcta ttgaagttat taattggaaa tatgaaggta
      481 aatattcaat atacaatcta cctccatggg aggaaattaa aaagaagaat ttttcactat
      541 caaaagaaga taaaagaaaa aattttataa gttttataaa cgacaataaa gaattgatag
      601 gttttataaa cttattagat gaaggaagtt ctgtattctt tggtattggt ataaaaccag
      661 agtattgcgg aatgggaatt ggtaaagaaa taattagcct aggattacaa gaatgtagaa
      721 ataaatatag tactaaacca attgtattaa atgtgagaat ctggaacatg agagctgtaa
      781 aatgttatga atctcaagga tttaaaatag ttgagactaa ggtacagaaa acgcatttag
      841 gggatggaga attttttgtt atgaggtatc aatagtttat aacaatagtc ctattgttaa
      901 tacaacaatt aaaatgaaaa aatacatgta tgtaggtcat ggcaataagt gtctttgaaa
      961 tataaatttg agagcttttg ataaaatttt gtatacatat atattttata ctacggatat
     1021 ataaatctat ctacatatat aaagagattg taagtaggtt tgtgtatata aaaatagatt
     1081 tatatttcat aaatgtactt tttatttgta ttacctttat tttatctaag ctctcatttt
     1141 attatctaat cttccttttt attggtttta ttaagagaga tgcaattatc aacacaacac
     1201 ttccaagtat cagccaatat ttctcatcaa aactaccttg actcatagac attataaaaa
     1261 attttccaac atacttgcct ttagtcatca tatttaaaat taaatatcct atagatatca
     1321 tgtaacctat gattgtatta ttaaataaat ttgagaaaaa cattcctaaa cttcctataa
     1381 acatagctga tgcgaaactt ccagtaatat actcaactac tggaaaatta catccaagat
     1441 atattaaaac atttacaaaa cctatgatta ataccactat acctataatt gacatcagta
     1501 tcctaatgaa ttgtacaata acttgagata atgattttga ggatactatc tcatcaattt
     1561 cttttgattg ctctagtgaa aatacaggta ccaacattac catccctatt attgcaacat
     1621 aattttctaa acatatagct gttgaaattc tatctaaatt agctacacct tgtattaaag
     1681 gaatcataca tagcaatata attgaacata ttaaattagt taaagtgctt aatttgaagt
     1741 tttcttttat tagaactaaa cagttttgaa aactttgacc aaacatcaag attccccctt
     1801 cttttttgct catataagaa tatagctaac aaaacaagca ccaatgctcc caagcttatc
     1861 acaattctat taatcataat ttgattgaag ttttgtataa taatatctcc atttaataca
     1921 ctgttatgtc ttatcattag tcccattgtt gaataatcac ctttgagtcc tgtagaattt
     1981 atactgtaaa accaccaaat taattgcaca ataattgcta ctggtgtatc tgttaatata
     2041 gtaatactca ttcctacagc tgtaactatc ataagagttg gtagtatcca ccataatgta
     2101 tattttataa atgctaagtt atcaatagat atacctttat ctttagcaaa gaaaatcaaa
     2161 ggaagtaaat ctttcatacc taaaattaat actggcaata tcatcataaa gacaagtgca
     2221 aaatatcttg ataaaactat tttgcatgaa gatacttgtt ttgaataaat aatctctctc
     2281 attcttgaac gcttatcttt aagtgacata aaaactgcca caaatactgg aaatatactt
     2341 aaaactatac ctatataatc acaaaaaatt cttgcatacc cattactaac tttatctttt
     2401 tgtattgtat agttgtaatt ctgcatagct tcattatagg taactggaac tactccaaat
     2461 ctttgtaact tgtcaaaagc atatgcagac cctcctccta aaatgtcatc tactctttgc
     2521 attatttctt caaacctttc aaaagatact ttagaagaat ctatttttat actttcacca
     2581 gttttccaaa ttaaatttat ttcactaata cttttactag ttatttcttc tatatatttt
     2641 tctatggtct tcgttttatt tttgtctaat tttatatact tagctactcc tgttggatac
     2701 gttgtatatg tgttttcttg atattctcta attaggttta tagttgcatt tggaatgact
     2761 ttttctgttt tcatagattc tttttgacca tatggatatt gattgtgtac agagtaatat
     2821 tcctcaatat tctctacttt ttgtggagca actaatggat ttccatcatt aggaatattt
     2881 ttacttacat catttgaaac tgaactccca agttgagaaa tatagaaaaa aactaaaact
     2941 acaataaaaa tataatatac catactcttt aatatatttt tacattcttt tataaataga
     3001 ttcatatatt atctcctccc attataagca tatagccatc ttcaatagtt ggattacaat
     3061 ttactgctgt ctcaaaaggt ttatcatctg atataatcct aagtgtagcc tttccaccat
     3121 gagtcaacat tcctataact ataaatctac tttttatatt ttcaatgtct tttttatcta
     3181 cttcaatact atatactttc ccttcagcta gttttgctaa tgcttctgtt gagccattgt
     3241 atatgacttt tccactatcc aatatagcta cgttttcaca tgtagcttct atatctccaa
     3301 caatatgtgt agataaaata acgattctat catttgcaat ttcgcatagt aaatttctaa
     3361 atcttatacg ttcttctggg tcaagacctg ctgtcggctc atctactatt aaaactctgg
     3421 gattatgaat taatgcttgt gctatcccaa gccttctatt catacctcca gataaagatt
     3481 ttactttcac attttgataa ttagtaaggt ttactttgct taataacttt gctataattt
     3541 ttcttctttt tcttagtggt atatccgata aaactgctaa ataatccatt gctttatata
     3601 ctgtcatgtt aggatacatt gaaaaatctt gtggcaaata accaaccata tttctaattt
     3661 ccttagtatt ctcaatggga actccacaca tatgtattga accttctgtt tttgataata
     3721 aagtggctaa aattctcata aatgtggttt ttcctgctcc atttcttcct aaaagaccaa
     3781 acataccact gtcgatttca agatttatat tatcaagagc ttgttttttc ccaaatcttt
     3841 tactaagatt ggatactgct attttcattt ccatatataa atacaccccc aaaaataatt
     3901 ttatagaatt ttatttttat aaatttctta ctgtattttc ata
//
LOCUS       DAEPXF010000264         3824 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain HB27
            SAMN13376389-rid12479423.denovo.272, whole genome shotgun sequence.
ACCESSION   DAEPXF010000264 DAEPXF010000000
VERSION     DAEPXF010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13376389
            Sequence Read Archive: SRR10518432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3824)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3824)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001069624.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001069624.1
            Genome Coverage       :: 315x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/15/2021 01:54:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,165
            CDSs (total)                      :: 4,076
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 89
            rRNAs                             :: 10, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 10 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 19 of 69
            Pseudo Genes (incomplete)         :: 44 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 10 of 69
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3824
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13376389-rid12479423.denovo.272"
                     /strain="HB27"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="China: Hebei"
                     /collection_date="2017"
     gene            46..1701
                     /gene="ilvD"
                     /locus_tag="KRL67_003662"
     CDS             46..1701
                     /gene="ilvD"
                     /locus_tag="KRL67_003662"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020457899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HBH1524496.1"
                     /translation="MRSDIKKGIEGAPKRALMYGMGLTKEEIERPLIGIVNAQNEVIP
                     GHLHLDEIAEAAKNGVRMSGGLPLEFPAIGVCDGIAMGHVGMNYSLASRELIADSIEA
                     MAMAHGFDALVLIPNCDKIVPGMLMAAARLNIPSIVVSGGPMLPGKKNGKVYDFNSAM
                     EGVGACKDGTVSEEELEELAMNSCPGCGSCSGLFTANSMNCLTEALGMGIPYNGTAAS
                     HSGERKRIAKYAGMYVMELLKNDIKPRDILTIDAFKNAIAVDMAMAGSTNTVLHLPAI
                     AYESGIELNLDFFDEISEKTPCLTKLSPSGKHHIEDLHMAGGIPAIMNELSKINGINL
                     DCKTVTGKTIGENIRNCEIENEEVIHTLKNQYSNQGGLAILKGNLALNGAVVKKSAVA
                     EEMLVHEGPARVFNSEEEAVNAIFAKEINKGDVIVIRYEGPKGGPGMKEMLSPTSAVA
                     GMGLDKHVALLTDGRFSGATRGASIGHISPEAMEGGLIGLVEEGDIIFINIPDKKLEL
                     KVDEVEIENRKLKFKPLEPKIKHGYLSRYAKLVTSANTGAVLK"
     gene            2047..2676
                     /locus_tag="KRL67_003663"
     CDS             2047..2676
                     /locus_tag="KRL67_003663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBH1524497.1"
                     /translation="MNREEKSKNSKEKIIQSAFSLFSSKGYDSTSTQDIINLSGLSRG
                     AMYHHFKTKEDILRSVTKELYSQMNNFLENLVADDTLTANEKIIELVVHSANDYTRRK
                     MVHCSWLEKIPFALIEEVRNLNNVVAPNIAKIIKQGVENKEFSCEYPKELAEMLVFSI
                     DILLDPVLFKREYSEVCNKLDFLLFMLKKMDTPLIDECGIKKLKDLFKQ"
     gene            2702..3514
                     /locus_tag="KRL67_003664"
     CDS             2702..3514
                     /locus_tag="KRL67_003664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBH1524498.1"
                     /translation="MFYNAKSCQMMIGSNTVNYIEFGSGKKTLIILPGLGDGLFPLHG
                     KIQAIAFAFRYKQFAKHYKVYVFSRKNQITEKYSTRDMAKDQSDIMKKLGIMKAEVMG
                     VSQGGMIAQYLAIDYPELVEKLVLAVTSSKQNDTIQNVICSWIDMAKKQNYNGLMIDT
                     ANKSYSERYLKKYRLFIPLLGKVGKPKDFKRFIIQATSCIEHNAFSELNKITCPTLII
                     GGANDKIVGNNASFHLAEKIKKSEIFIYEGLGHATYEEAQDFNERVLEFLNK"
BASE COUNT         1477 a          447 c          733 g         1167 t
ORIGIN      
        1 taataatatt atgaaaattt aaaaaagtaa ggaagaggta atattatgag aagtgatata
       61 aaaaaaggaa tagaaggagc tccaaagaga gcgttaatgt atggaatggg tcttactaaa
      121 gaagaaattg aaagaccatt aataggtata gtaaatgcac aaaatgaggt gattcctggt
      181 catctgcatc ttgatgaaat agcagaagct gcaaaaaatg gagtaagaat gagtggtggt
      241 ttgccattag aatttcctgc tataggtgta tgtgatggca tagctatggg gcatgtcgga
      301 atgaattatt ccctggcttc aagagaatta atagcagatt caatagaagc tatggctatg
      361 gcacatggtt ttgatgcttt ggtacttata cctaattgtg acaagatagt gccaggtatg
      421 ttaatggctg ctgcaagact taatatacca agtattgttg tgagtggagg gcctatgctt
      481 ccaggtaaaa aaaatggaaa agtatacgat tttaactctg caatggaggg tgttggagca
      541 tgtaaagatg gaacagtaag tgaagaagaa ttagaagagt tagctatgaa ctcttgtcct
      601 ggatgtggtt catgttctgg acttttcaca gcaaatagca tgaattgttt aacagaagct
      661 ttaggcatgg gtataccata taatggtact gctgcgtcac attctggaga aagaaaaaga
      721 atagcaaaat atgcaggtat gtatgttatg gagttactta agaacgacat aaaacctaga
      781 gatattttaa caatagatgc ttttaaaaat gctatagctg tggatatggc aatggctggt
      841 tctacaaata cagtacttca cttacctgca atagcttatg aatcaggaat agagcttaac
      901 ttagattttt ttgatgaaat aagtgaaaaa actccttgtt taacaaaatt aagtccaagt
      961 ggaaaacatc atattgaaga tttacatatg gcaggaggaa taccagctat aatgaacgaa
     1021 ctttcaaaga taaatggaat aaatttagat tgcaaaaccg taacaggcaa gactataggc
     1081 gaaaatataa gaaattgtga aatagaaaat gaagaagtaa tacatacatt aaagaaccaa
     1141 tatagtaacc aaggtgggct tgcaatattg aaaggaaatc ttgctctaaa tggagctgtt
     1201 gtaaaaaagt cagcagttgc agaagagatg ttagttcatg aaggaccagc aagagttttt
     1261 aattcagaag aagaagctgt aaatgctatt tttgctaaag aaataaacaa aggtgatgtt
     1321 atagttataa gatatgaagg tccaaagggt ggtccaggaa tgaaagaaat gctatctcct
     1381 acatcagcag ttgcaggaat gggacttgat aagcatgtag cacttcttac tgatggtcgt
     1441 ttttcaggag caactagagg agcatctata ggccatattt ctccagaagc tatggaaggt
     1501 ggtttaatcg gactagttga agaaggagat ataattttta taaatatacc agacaaaaaa
     1561 ttagagttaa aagtagatga agttgaaata gaaaatagaa aattaaaatt taaaccttta
     1621 gaaccgaaaa taaagcatgg atacttaagt agatatgcta aattggtaac atcagcaaat
     1681 acaggagcag ttttaaaata gtggactata tttacgaaaa ttagaaaaag ttaagaaaat
     1741 attaaatgga tatcttaggt gttacagcac taatgagata tcctttttat tttgtatgaa
     1801 aaacacagca aaatcttgat tatctttgaa aacaatacat ctctattaaa ggaatatgag
     1861 tacagcatag agatgtatct tttcgaccct gtgaggtaag acaacttttt aaaagtttga
     1921 tgtggaatgc ctttcattcc ctctgaacta tgaagaaatt aaaagtcagt taattcttga
     1981 cataataaat attaaaatgt ataataaaaa aacaaacaga tagtctgttt ttttaagggg
     2041 ggcaatatga atagagaaga aaaaagtaaa aatagtaaag aaaaaattat tcaatcagca
     2101 ttttcactat tttcgtctaa aggatatgat tcaacatcta cacaagatat tatcaattta
     2161 tctggtttat ctagaggtgc aatgtatcat cactttaaaa ctaaagaaga tatacttaga
     2221 agtgtcacaa aggaacttta ctcacaaatg aataattttt tagaaaatct tgttgctgat
     2281 gacacactta cagcaaatga aaaaataata gaattggttg ttcatagtgc gaatgattac
     2341 acacgtagaa aaatggtaca ttgtagctgg ttagaaaaaa taccattcgc tttaatagag
     2401 gaagttcgta atctcaacaa tgtagttgca cccaatattg ctaagataat taaacaaggt
     2461 gttgaaaata aagaattttc ttgtgaatat ccaaaggaat tagctgaaat gctcgttttt
     2521 agtattgata ttttacttga ccctgtatta tttaaacgtg aatacagtga agtatgcaat
     2581 aagttagatt ttttattgtt tatgttaaaa aaaatggata ctcctctaat tgatgagtgt
     2641 ggtattaaaa aacttaagga tttatttaaa caatgataat aggaaaagtg aggaatagac
     2701 tatgttttat aatgcaaaaa gttgtcaaat gatgataggc agtaatacag tgaactatat
     2761 tgaatttggt agtggtaaga aaacattaat tattttgcca ggattaggag atggtttatt
     2821 tcctttacat ggaaaaatac aggcgattgc ttttgctttt agatataagc aatttgcaaa
     2881 acattataag gtttatgtgt tcagtagaaa aaatcaaatt acagaaaaat attccacgag
     2941 ggatatggct aaagaccaat cagatataat gaaaaaactt ggaattatga aagcagaagt
     3001 aatgggtgtt tctcaaggtg gaatgatagc tcaatatctg gcaattgatt atcctgagtt
     3061 agttgagaaa cttgtgttgg cagttacttc ttcaaagcaa aatgatacta tccaaaatgt
     3121 tatttgtagt tggattgata tggcgaagaa gcagaattac aatggtttaa tgattgacac
     3181 agcaaacaaa tcctattctg aaagatattt aaagaaatac cgattattta ttcctctttt
     3241 aggaaaagta ggtaaaccaa aggattttaa acgatttatt attcaagcga cctcttgcat
     3301 agaacataat gccttttccg aattaaataa aataacatgt ccaaccctaa ttataggagg
     3361 tgctaatgat aaaattgttg gaaataatgc ttcttttcat ttagcagaaa aaattaaaaa
     3421 aagcgaaata tttatctatg agggattggg acacgctaca tatgaagaag cacaggattt
     3481 caatgaaaga gttttagagt ttttaaataa gtaaacacct ttgttaaaaa tatacatata
     3541 aacattggaa atttaatgtt aaaaatagaa acatgaaaag atgcttgact ggtattttta
     3601 ctatgcctaa caaatttttg atgcattatc tactataaat acaaatatag cttcaatgtg
     3661 attatatgtt ggtgtattgg taaagcactt atacaaacag aggaattttg taaattcaga
     3721 ttatacccat atttgttaat ttatgaaaat ataatcaaaa tttttatgag cttttatgag
     3781 aaaaaatata atgtttgtat acaaaaatat taaagatatt ttag
//
LOCUS       DAEPXF010000265         3809 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain HB27
            SAMN13376389-rid12479423.denovo.273, whole genome shotgun sequence.
ACCESSION   DAEPXF010000265 DAEPXF010000000
VERSION     DAEPXF010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13376389
            Sequence Read Archive: SRR10518432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3809)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3809)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001069624.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001069624.1
            Genome Coverage       :: 315x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/15/2021 01:54:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,165
            CDSs (total)                      :: 4,076
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 89
            rRNAs                             :: 10, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 10 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 19 of 69
            Pseudo Genes (incomplete)         :: 44 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 10 of 69
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3809
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13376389-rid12479423.denovo.273"
                     /strain="HB27"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="China: Hebei"
                     /collection_date="2017"
     gene            complement(<1..755)
                     /locus_tag="KRL67_003665"
     CDS             complement(<1..755)
                     /locus_tag="KRL67_003665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBH1524499.1"
                     /translation="MMYLVKAEMIKILKSKSVWIIWLFLLFFGFLLIRKFDVLDTYAD
                     IFYKIEGSIPLIGLIMFIITSGNYIKEYDSNMTGLINTTKKGKKSVVLSKLLANGLTL
                     SIINISFILLLGIRGFSFFDFKNLNEPIHNLWYFGNSNLNLTVIQMYIIVILTTIFAS
                     FIFAQIGLTLSSIFKSAVIPFILGGLIIAIPYFSVGFIPDKAIKFMSLTPNWIMMSQQ
                     IVRYNVPSILIVFSIVISIILMIVLTKITYENFT"
     gene            complement(761..1648)
                     /locus_tag="KRL67_003666"
     CDS             complement(761..1648)
                     /locus_tag="KRL67_003666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011948651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBH1524500.1"
                     /translation="MNKLEIEKLSKIYGKKVANDKITVTLENGVYGLLGPNGAGKSTL
                     MKQITTLIKPDEGQILYNGEDIFKMDDDYRNILGYLPQEFGVYKNFTAKEFLQYIGAL
                     KGLRGKHLNIKIGELLDLVGLYDVRNKSIGKFSGGMKRRVGIAQVLLNDPKIIVLDEP
                     TAGLDPQERARFRNLIAKMSRDKIIILSTHIISDIESVAKETIMIKDGKLLMKGTHKD
                     ILSHMENKVYNVQVKNECEVDDIQSKYKVVSIQSDIDSIVLRIVSEDIPCGANIQPVQ
                     AKFEDVYMFYFDLDDAREV"
     gene            complement(1662..2684)
                     /locus_tag="KRL67_003667"
     CDS             complement(1662..2684)
                     /locus_tag="KRL67_003667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH1524501.1"
                     /translation="MVNWEIKKLAKSKSMFISLGILVLILIISIFITPVLETESYYID
                     KEKGRIEDTRSGIDIGNEKFQNKINVLKQFSNQTDTDEFSKKIELMSKKKLDNLSVNE
                     YKDISFWKVFNYRVTNSLINVGMLIIISIIISNIYVDEVVTSVKDIILSSKEKKKALK
                     SKIFVALLVPLVIYSVYLMGIFIITCMQQGVPVNGDLESFRVVDNVVALKTNLSITKY
                     VILNIGIELLMFEGWAMIAVFGSFISKSSISSISFFIFIISITKIMSVIHILPKKLLS
                     VISNVNYYDLIFGFNKIIGNYLGDIMIFKVNIDIVNIAIGLLMAILIGAISLCFLVIK
                     SDYISR"
     gene            3252..3428
                     /locus_tag="KRL67_003668"
     CDS             3252..3428
                     /locus_tag="KRL67_003668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH1524502.1"
                     /translation="MFIGEIDKCTHILTAYISSSYDYCNFLDTQLDDFISEYGETVVE
                     TCLHQVLLLVSRYN"
BASE COUNT         1363 a          573 c          372 g         1501 t
ORIGIN      
        1 gtaaaatttt catatgttat tttagttaac actatcatta atataattga gattactata
       61 gaaaaaacta tcaaaatact tggtacatta tatctaacaa tttgttggct catcataatc
      121 caatttggag tcaatgacat aaattttata gctttatctg gtataaaacc tacactaaag
      181 tatggaattg ctattatcaa tccacctagt ataaatggaa ttacagcaga tttaaatata
      241 gatgaaagtg ttaagcctat ttgtgcaaat ataaaagagg caaaaatagt agtcaatata
      301 acaattatat acatttgtat tactgtaaga tttaaattac tatttccaaa gtaccataag
      361 ttatgaatcg gttcgtttaa atttttaaaa tcaaaaaaac taaaacctct aattcctaat
      421 aacaatataa aagaaatatt aataatagat aaagttaatc cattagctaa tagttttgat
      481 aatactactg atttttttcc ctttttagtt gtatttatta aacctgtcat attagaatca
      541 tattctttaa tgtaatttcc agaagttatt ataaacatta tcaaacctat tagaggtatt
      601 gagccttcta tcttgtaaaa tatatctgca taagtatcta aaacatcaaa ctttctaatc
      661 agaagaaatc caaaaaatag taaaaacagc caaattatcc aaacactttt tgattttaat
      721 atttttatca tttcagcttt aactaaatac atcatttcta ctacacctcc cttgcatcat
      781 ccaaatcaaa ataaaacatg tatacatctt cgaattttgc ttgaactggt tgtatattag
      841 ccccacatgg aatatcctct gaaacaattc taagtactat tgaatcaatg tcactttgaa
      901 tacttacaac cttatattta gattgtatat catcaacttc acattcattt ttaacttgaa
      961 cattatatac tttattttcc atgtgactta atatgtcttt gtgtgttcct ttcattagga
     1021 gtttgccatc tttaatcatt attgtttcct ttgcaactga ttctatatcg gaaattatat
     1081 gagtagaaag tattattatc ttatctctag acatttttgc tattaaattc ctaaatctag
     1141 ctctttcttg tgggtcaaga cctgctgttg gttcatcaag gactattatc ttagggtcat
     1201 ttagtaaaac ttgtgctatc ccaactctcc tcttcatacc tcccgaaaat tttcctatag
     1261 acttatttct cacatcataa agacctacta aatctaataa ttctcctatc ttaatattta
     1321 aatgttttcc ccttaaacct tttaaagctc caatatactg taaaaattct tttgctgtaa
     1381 aatttttata aactccaaat tcttgtggca aatatcctaa aatatttcta taatcatcat
     1441 ccattttaaa tatatcctca ccattataaa gtatctgacc ttcatcaggt tttatcaatg
     1501 ttgttatttg tttcattaat gtagattttc ctgctccatt tggtcctaga agaccataaa
     1561 ctccattttc cagagttact gtaatcttgt cgtttgctac ttttttacca tatatctttg
     1621 aaagtttttc tatttctaat ttattcatct taactcctct tttatctact tatataatct
     1681 gattttatta ctaaaaaaca taatgatata gcacctatca atattgccat caaaaggcct
     1741 attgctatat tgacaatatc tatattcact ttaaatatca taatatcacc tagataattt
     1801 cctattattt tattaaaacc aaatatcaaa tcataataat ttacatttga aataactgaa
     1861 agtaattttt taggtaatat atggattact gacattatct tagttataga tataataaat
     1921 ataaaaaaac ttattgaaga tattgaagat ttacttataa aagacccaaa tacggctatc
     1981 atggcccatc cttcaaacat caaaagctct atgcctatat ttaatattac atactttgtt
     2041 atacttaaat tagttttcaa tgcaactaca ttgtctacta ctctaaaaga ttctaagtct
     2101 ccattaacag gtactccttg ttgcatacaa gttataataa atattcccat taaataaaca
     2161 gaataaatta ctaatggaac taataaagca acaaatattt tagattttaa tgcttttttc
     2221 ttctcttttg atgatagtat aatatctttt actgaagtaa cgacttcatc tacgtaaata
     2281 ttggatataa ttatagatat aatgattaac attcctacat ttataagtga atttgtcact
     2341 ctatagttaa atactttcca aaaactaata tccttatatt cattcacact taaattatca
     2401 agttttttct ttgacattaa ttcaattttt ttagaaaatt catctgtatc tgtttgattt
     2461 gaaaactgct ttaaaacatt aatcttattt tgaaatttct cattacctat gtcgatacca
     2521 cttctcgtat cttctatacg tcctttttct ttatctatat aataactttc tgtttctaaa
     2581 acaggtgtaa taaagatact aattattagt attaaaacta atatccctag agatataaac
     2641 atactcttag atttagccaa tttttttatt tcccaattta ccaatatatt tctcctccta
     2701 aatcatttta attacacata tatattaaaa cttaaatctt atataaatat aatcttaacc
     2761 ttaaataaag cttaaattat tttataattt acaggtgtgt ttattattgg agttacattt
     2821 attatttttc tgggaaatta tacattttca attatttttg atatttttat tgattggaaa
     2881 ttttattgtt gttcaaattg tagttattct ttaaatttaa agatggagtt ttttaagaat
     2941 agaacaccta ctgtccttgt tgtggaaaga ttattaaata gttgcatata actttattta
     3001 tcttgaatct tctttttaaa aacaattata attttagaat attttctatt ctcaaacaag
     3061 catgttgaat aatgaagcat cttctagtat tatatacttg tagaataaaa ctaaattggt
     3121 aaaactggga atatttaacc ttaaggagtt aagttataga aactagtact aaaaaatttt
     3181 ataaattatg ttaattgact aatagaatgg gactttttat ttttactaaa ttttttaggg
     3241 ggatggataa tatgtttatt ggagaaatag acaaatgtac acatatacta actgcttaca
     3301 tcagtagttc atatgattat tgtaattttt tagatacaca gttagatgat tttatatcag
     3361 aatatggaga aacggtagtt gaaacctgct tacatcaagt cttattatta gtaagtagat
     3421 acaattaaaa ttaaattttt ctagaaaaac ctttttagat ttatctactc taataaatat
     3481 ataatttttt gtgctttata agtaactata acttctgcta aagtaaagat gtattgggag
     3541 tacatcttta ctttcccatt atagaagtta aaagcataca cgataaaatc aattatacaa
     3601 tacaaattat atacttctta tatctaactt aatttacaag aatcatccta tatgtatatt
     3661 ctgcaatcca ctaaacatgg ctctaaatct ttactatttt atacattctc tataaatcat
     3721 acaaaccata tataaaatcc ccctaatatg tttaaaccat atataaaata acaccaatat
     3781 ttagaatatt caatatcaac tgcataact
//
LOCUS       DAEPXF010000266         3804 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain HB27
            SAMN13376389-rid12479423.denovo.274, whole genome shotgun sequence.
ACCESSION   DAEPXF010000266 DAEPXF010000000
VERSION     DAEPXF010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13376389
            Sequence Read Archive: SRR10518432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3804)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3804)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001069624.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001069624.1
            Genome Coverage       :: 315x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/15/2021 01:54:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,165
            CDSs (total)                      :: 4,076
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 89
            rRNAs                             :: 10, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 10 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 19 of 69
            Pseudo Genes (incomplete)         :: 44 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 10 of 69
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3804
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13376389-rid12479423.denovo.274"
                     /strain="HB27"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="China: Hebei"
                     /collection_date="2017"
     gene            81..1193
                     /locus_tag="KRL67_003669"
     CDS             81..1193
                     /locus_tag="KRL67_003669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HBH1524503.1"
                     /translation="MNAFIRKRNKNYVVYLEFEDEKTGKRKQKNMGTFDKKREASKRL
                     NEVKESLYKDGFLVPSEITVSEFLLDFLNKYSENISLATYNNYVSICKNYINPSIGKY
                     KIQDLHPVHIQNYIDKLSYKLNPQSIKIHINILKLAIKRAYRLKLIKENVMDSIEAPR
                     YKKFKNEIYDREQMIKLLDLAKNTEMELPINLAIGLGLRISEVLGLTWDNIDFEENTI
                     TVNKITSRINGSVILKEPKTESSVRKISSPKELMFLLKEYKIKQNKNLLKSSIRNNNN
                     LLFFNKKCEPIAEDVMSKKFKRFLEKNELPHIRFHDLRHSHVTLLINSKVPIKVISER
                     VGHSNISTTLSVYSHVLKEMDKEASDKISESLFNAN"
     gene            complement(1401..1850)
                     /locus_tag="KRL67_003670"
     CDS             complement(1401..1850)
                     /locus_tag="KRL67_003670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH1524504.1"
                     /translation="MVKKRLVAVVLSLAMISVGNFSYAHSGGYNSSGGHHNKNKSELT
                     RYYCGGHSAHQHNKGFCPYISTKENSGVTVSSVHNEKNHKNYFAEKGYNKGYEDGCKG
                     LYNLSLTEAKSDNSYEKGYLIGYNEYKAKKVDDVMKKMLDIPMLASI"
     gene            complement(2049..3674)
                     /locus_tag="KRL67_003671"
     CDS             complement(2049..3674)
                     /locus_tag="KRL67_003671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBH1524505.1"
                     /translation="MLQVTGVGLRFGDKELFKDVNLKFTKGNCYGIIGANGAGKSTFL
                     KILSGEIEPNTGSISITDKERMSVLKQDHFEYEEETVLNVVIRGHERLWNIMKEKDAL
                     YMKEDFNEEDGIKAAELEGEFAELDGWDAETNAEKILMGLGITKDMHYKQMKELVGGE
                     KVKVLLAQSLFGKPEILLMDEPTNHLDFKSINWLNNFIMDLEESIVIIVSHDRHFLNQ
                     ICTNIVDVDFGKIQMYVGNYDFWYESSQLALQLAKDQNKKTEEKIAQLKEFIARFSSN
                     ASKAKQATSRKKQLEKLEVEDIQPSRRRYPYVGFTPAREIGNEVLEVHNLTKTIDGVK
                     VLDNVSFRLDRDDKVVFMGDEIATTALFNIVMGELEPDSGEYKWGVTTSQDYLPKNHN
                     KFFDGVEYSLVDWLRQFSEEKSESFIRGFLGRMLFSGEEALKEAQVISGGEKVRCMLS
                     KLMLSNANVLVLDDPTNHLDLESITSVNKGLEKFPGVLLFTSHDHEFISTIANRIIEI
                     TPNGIMDRKMDFDEYLESKEIQDQLAKMYGKDK"
BASE COUNT         1327 a          610 c          427 g         1440 t
ORIGIN      
        1 tgtatgcaac tctatttcaa aattattttg tgatatatat gttataataa atgtataaaa
       61 ttttgattgg tgggttatat atgaatgcat ttattagaaa aagaaataaa aactatgttg
      121 tttacctgga atttgaagat gaaaagacag gtaaaagaaa gcaaaaaaat atgggtactt
      181 ttgataaaaa aagggaagca agtaaaagac taaatgaggt aaaagaaagc ttatataagg
      241 atggtttttt agtaccaagc gaaattacag tttcagaatt tctattagat tttctaaata
      301 aatatagtga aaatatatct ttagctacat ataataatta tgttagtatt tgtaagaatt
      361 acattaatcc atctattgga aaatataaaa tacaagactt acatccggtt cacatacaaa
      421 actacataga taaactatct tataaactaa atcctcaatc aattaagatt catataaata
      481 tattgaaact tgcaataaaa agagcttata gactcaaatt aataaaagaa aatgttatgg
      541 atagtataga ggctccaaga tataaaaaat ttaaaaatga gatttatgat agagaacaaa
      601 tgataaaatt attagattta gcaaaaaata cagaaatgga attacctatt aatttggcta
      661 ttggattagg tcttagaatt tcagaagttt taggtctaac ttgggataat attgattttg
      721 aagaaaatac aataacagta aataagataa cttcccgaat taatggttct gttattctca
      781 aagagccaaa gacagaaagc tctgttagaa aaatatcttc tcccaaagaa ttaatgtttt
      841 tattaaaaga atataaaata aaacagaata aaaatttatt aaaatcttcc attagaaata
      901 ataataattt gttgtttttt aataagaaat gtgaacctat tgctgaagat gttatgagta
      961 aaaaattcaa aagattttta gagaagaatg aattgcctca tattagattc catgatttaa
     1021 gacactcaca tgttacttta cttataaatt ctaaagtacc tataaaggtt atatcagaga
     1081 gagtaggaca ctcaaatata agtactactc taagcgttta ttctcatgta cttaaagaaa
     1141 tggataaaga agcttctgac aaaatttctg aaagtttatt taatgctaat taatattaaa
     1201 ctatcttagc tttaaattag cttagcttat tgagatagat tcattaatat taaatgtatc
     1261 tatctcaaat tagtaactac ttaaataatc tgttttattt taatttatca cctcagttag
     1321 tatacttttt tatgaaaaat tatactttac aataaaaaag atatttcctt atagaaaata
     1381 tctttttaaa tactatctta ttatatactt gctagcattg gaatatctaa catcttcttc
     1441 attacatcat ctactttttt tgctttatat tcattgtatc caattaaata tcctttttca
     1501 tatgaattat cagatttggc ttctgtgaga cttaaattat aaagcccttt acacccatct
     1561 tcataacctt tattataccc tttttctgca aaataatttt tatgattttt ttcgttatgt
     1621 actgaagata ctgttactcc agaattttct tttgtactaa tataaggaca aaaaccttta
     1681 ttgtgttgat gtgctgaatg tcctccacaa taataacgcg ttaactctga tttatttttg
     1741 ttatgatgac cccctgatga attataccct cctgagtgag catatgaaaa attccctaca
     1801 ctaatcatag ctaaacttag tactacagct actaaccttt tcttaaccat aatctattcc
     1861 ccccaaaatg ttatatattt ttattatatc atctactata ttatatttaa attcttaata
     1921 tacaaaatat tactctattc tactaataaa tatatttaaa caacataaaa aacctcctaa
     1981 tatttgatta aaaacttaca cacttatgca tagcatgtct caaatattaa aaggttttat
     2041 actaataatt atttatcttt tccatacatt ttagctaatt ggtcttgtat ctctttactt
     2101 tctaaatact catcaaagtc catttttcta tccattattc catttggagt tatctctata
     2161 attctatttg ctatagtaga tataaattca tggtcatgag aagtaaataa aagcactcct
     2221 gggaactttt caagtccttt atttacagaa gttatacttt ctaggtctag atggttagta
     2281 gggtcatcta atacaagtac atttgcattt gaaagcatta gcttactaag catacatcta
     2341 accttttctc ctccagatat aacttgagct tcttttaatg cttcttcacc agagaataac
     2401 attctaccta aaaatcctct tataaaactt tcactttttt cttcagagaa ctgtctaagc
     2461 cagtcaacca aagaatattc tactccgtca aaaaacttat tatgattttt tggaagataa
     2521 tcttgagaag tagttactcc ccatttatac tctccactgt ctggctcaag ctctcccata
     2581 actatgttga aaagtgcagt agtagctatt tcatcaccca taaatactac tttatcatct
     2641 ctatccaatc taaatgacac attatcaaga accttaaccc catctatagt tttagttaaa
     2701 ttgtgtactt ctaaaacttc attcccaatt tctcttgcag gtgtaaatcc tacatatgga
     2761 tatcttcttc ttgatggttg tatatcttca acttctaatt tctctaactg tttctttctt
     2821 gatgttgctt gtttagcctt tgaagcatta gaactaaatc tagctataaa ttcctttaat
     2881 tgagctatct tttcttcagt ctttttattt tggtccttag ccagttgtaa agccaattga
     2941 cttgactcat accagaaatc atagtttcca acatacattt gtattttacc aaaatctacg
     3001 tcaactatat tagtacatat ttgatttagg aaatgtctat catgtgatac tattataact
     3061 atagactctt ctaaatccat tataaaatta tttaaccagt ttattgattt aaaatctaag
     3121 tggtttgtag gttcgtccat taatagtatc tcaggcttac caaaaagtga ttgtgctaac
     3181 aataccttaa ccttttcccc accaactagt tctttcattt gtttataatg catatctttc
     3241 gttatgccaa gacccataag tattttctca gcattagttt ctgcatccca tccgtcaagt
     3301 tctgcaaact caccttcaag ttcggcagct tttatcccat cttcttcatt aaagtcttcc
     3361 ttcatataca atgcatcttt ttctttcatt atgttccaaa gtctctcatg acctcttata
     3421 acaacattta aaacagtttc ttcttcatat tcaaagtggt cctgttttaa aactgacatt
     3481 ctttctttat cagttataga tatactacct gtattcggct ctatttctcc tgataatatt
     3541 tttaaaaaag tagattttcc cgcaccatta gctcctatta ttccatagca attaccttta
     3601 gtgaatttta agttgacatc tttaaatagt tccttatcac caaatctaag tccaacacct
     3661 gtaacttgta acattaatat attttccttt ctttttgctc ataatttatt ttatcataaa
     3721 actttgataa catttttatt atatattatc acgtaaatag tatatttttt atactaaatt
     3781 tgtgaaatat ttattaaaat aata
//
LOCUS       DAEPXF010000267         3800 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain HB27
            SAMN13376389-rid12479423.denovo.275, whole genome shotgun sequence.
ACCESSION   DAEPXF010000267 DAEPXF010000000
VERSION     DAEPXF010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13376389
            Sequence Read Archive: SRR10518432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3800)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3800)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001069624.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001069624.1
            Genome Coverage       :: 315x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/15/2021 01:54:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,165
            CDSs (total)                      :: 4,076
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 89
            rRNAs                             :: 10, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 10 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 19 of 69
            Pseudo Genes (incomplete)         :: 44 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 10 of 69
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3800
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13376389-rid12479423.denovo.275"
                     /strain="HB27"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="China: Hebei"
                     /collection_date="2017"
     gene            208..885
                     /locus_tag="KRL67_003672"
     CDS             208..885
                     /locus_tag="KRL67_003672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBH1524506.1"
                     /translation="MYNLLVVDDDVDILNINQIYFSNIGFNVYTSETAKDAMDIVESI
                     VLDCIILDISLPDIDGYKMCNDIKEKVNIPIIFLSNYEHEEEKVRGFLAGGDDYITKP
                     YSLKELELRIYARMRQYKNITSVPNVLKFSALVINVNSRKVTFDGESIDLTTMEFEIL
                     LFLAENKEQVFSKIEIYNQVWKMPDIGDQHTVQVHIAQMRKKINSLSREHQYIQTVWG
                     KGYKFVP"
     gene            957..2972
                     /locus_tag="KRL67_003673"
     CDS             957..2972
                     /locus_tag="KRL67_003673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HBH1524507.1"
                     /translation="MKKYNILKGTLIVLIVSLLAITGLNLLYKYDNKYTYKSIRASNG
                     ILTIDNDTFTKGKLVFLIDDWEFYNHKIFKPNDFTEQDIKPEYVFIGQYPDFSMRKGN
                     QSPYGNATYRMRIKNEGKDKLLSLELPEIFSASEVWINGEMVSKLGDVGTEKYRPKIK
                     NSVVSFIAKKDTEIIINVSNFSHYYSGLYYPPALGETKDISNMMFYRLLFYSIICFTT
                     FAIAIFSLSVWFLSNKSKLYLYFGCMCVFFAIHVSYPFVHLLGLPLVSFTYAVEDSSY
                     FMVVLCIICINGSISKIEENKVYRFLILPLGITMSIISIVIPIILLPYDMYFVNIYGK
                     LIDIYKYIVFIYILMSSLVSIYKKTCSEHISSYILVSVNTVFGISILFDAITSNRFEP
                     IFTGWQTEYCGFVIVILFSVMMINRNRIIIKENEKLTRNLEHEVEVRTNELTTLLNER
                     KKFLADVAHDLKAPVSAIQAFIDLIKVGNIHVDEETRTYLYAINQKSNEVQNRVRSLQ
                     EFTSQDKSIGISEKICLNDFIKEIYENNLPDTQACGVNFETYVLEKKIYAKINKEMLT
                     RAFENLIYNALTFTPFDGKIILSMDKEDGFAIIKISDNGKGIPPENIPKVFDRFFTER
                     DNNEPKGQGLGLYIVKSIIREHGGEIFVDSELGKETVFTIKLSLCFL"
     gene            3022..3702
                     /locus_tag="KRL67_003674"
     CDS             3022..3702
                     /locus_tag="KRL67_003674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBH1524508.1"
                     /translation="MQTKILIIDGDKDNCQKLKGFLEEKGISIDLAYNCEEAIGKIFS
                     NKYDLIFLEIILSDGDGWTLCKKIRNVTTCPIVYMTYINEDQSILNALNSGGDDYLIK
                     PLNLEILYAKVKAILRRMNSYVNNNENNTKLEEYNRTSGVIKLEDKIIKLTPTENKLL
                     NFFIENPEKTLTTKEIYEHVWMNEYLEDNYSVVVAVNGLRKKIEKDYKNPQKIITIRG
                     AGYYFNKE"
BASE COUNT         1514 a          359 c          644 g         1283 t
ORIGIN      
        1 cggagacttt aattagtatt taataatgaa tattaatatt taatacaata ttcaagaatt
       61 attcaaaaat acttaagtat tatagtctag tttgtaaaga tattataaaa taataatact
      121 agttatatat gaagataaaa cattataact tgtaataatt ataaaacttg agattatgat
      181 taaaatacct ttgaatggag tggtgtaatg tataatttac tggttgtaga tgatgatgtt
      241 gatattttaa atataaatca aatttatttt tctaatatag gttttaatgt atatacatct
      301 gaaactgcta aagatgctat ggatatagta gaaagtatag ttttagactg tataattttg
      361 gatatttcat tgccagatat agatggatat aaaatgtgca atgatataaa agaaaaggtt
      421 aacataccta taatattttt atctaattat gagcatgaag aagaaaaagt tcggggattt
      481 ttagctgggg gggatgatta tataacaaaa ccttatagtt taaaagaact tgaacttaga
      541 atatatgctc gtatgaggca gtacaaaaat attacatcag ttcctaatgt gttaaagttt
      601 tcagcgttag taataaatgt aaattcaaga aaagtaactt ttgatggaga aagtatagat
      661 ttaactacaa tggaatttga aatattattg tttctagctg aaaacaaaga acaagtattt
      721 tcgaagatag agatatataa tcaagtttgg aaaatgcctg atattggaga tcaacataca
      781 gtgcaggttc atattgcaca gatgagaaaa aaaattaatt ctttaagcag ggagcatcaa
      841 tacatacaaa ccgtatgggg aaaaggatat aagtttgtac cataagtgag atactgtaat
      901 taataaatag ttcttgtttt atttaatact actatgaaag agatgtggtt gatgcaatga
      961 aaaagtataa tatactaaaa ggaactctca ttgtattgat agtctctctg ttggcaataa
     1021 caggattgaa tcttctgtat aaatatgata ataaatatac atataagtct attagagcaa
     1081 gtaatggaat tttaactata gataatgata ctttcactaa aggtaaactt gtatttttga
     1141 tagatgactg ggagttttat aatcataaaa tatttaaacc aaatgatttt acagagcaag
     1201 atataaaacc agaatatgtt tttataggtc aatatccaga tttttctatg agaaaaggga
     1261 atcagtcacc atatgggaat gctacttatc gtatgagaat aaaaaatgaa ggtaaggata
     1321 agctactaag tttagagctt cctgaaattt tttccgcaag cgaagtatgg ataaatggag
     1381 aaatggtttc taaattagga gatgtaggaa cagaaaaata tagacccaaa attaaaaatt
     1441 cagtagtaag ttttattgca aagaaagata cagaaattat aatcaatgta tcgaattttt
     1501 cacattatta tagtggtttg tattatccgc ctgcattagg agaaacaaag gacataagta
     1561 atatgatgtt ttatcgatta cttttttata gtataatttg ctttacaact tttgcaatag
     1621 ctatattttc gttgtctgta tggtttcttt caaacaaaag taaactttac ttatattttg
     1681 gatgtatgtg tgttttcttt gccatccatg tatcctatcc ctttgtacat ttgttaggac
     1741 tgcctttggt aagttttaca tatgcggttg aagattcgtc atattttatg gtagttttat
     1801 gtattatttg tataaatgga agtatatcca aaatagaaga aaataaagtg tatagatttt
     1861 taattttacc attaggaatt acaatgagta tcatatctat agtgatacca attattcttc
     1921 ttccatatga tatgtacttt gtcaacatat atgggaaatt aatagacata tataaatata
     1981 ttgtatttat atatatatta atgtcttcgc tggtttcaat atataaaaag acatgctctg
     2041 agcatataag ttcttatatt ttagtttcag taaacactgt ttttggaatt agcatattat
     2101 ttgatgcaat tactagtaat agatttgagc ctatatttac agggtggcaa acagaatatt
     2161 gtgggtttgt aattgttatt ttattttctg ttatgatgat aaacaggaat agaataataa
     2221 taaaagagaa tgaaaaactt acaaggaatc ttgaacatga agttgaagtg agaactaatg
     2281 aacttacaac attgttgaat gaacgtaaaa aattcttggc agatgtagca catgatttaa
     2341 aggcacctgt ttcggctata caagcattta tagacttaat aaaggttggt aatattcatg
     2401 tagatgaaga gacaagaacc tatctttatg ctataaatca aaagtcaaat gaagtacaaa
     2461 atagagtacg tagtttgcaa gaatttacat cacaagataa aagcattggt attagtgaaa
     2521 aaatatgtct gaatgatttt attaaagaga tatatgaaaa taatttgcca gatacacaag
     2581 catgtggtgt aaactttgaa acttatgtat tggagaaaaa aatatatgca aagataaata
     2641 aagagatgct tacaagagca tttgagaatt taatttataa tgctttgacc tttactccat
     2701 ttgatggaaa gataatttta tcaatggata aagaagatgg atttgcaatt attaaaattt
     2761 ctgataatgg aaaaggtata cctccagaaa atataccaaa ggtatttgac agattcttta
     2821 cagaaagaga taataatgag ccaaaagggc aagggttagg gttatatatt gtcaaatcca
     2881 ttataagaga gcatggtgga gaaatctttg tagattcaga actaggaaaa gaaactgttt
     2941 ttacaataaa attaagtcta tgttttttat aataatagct tatttaatat aaaagaataa
     3001 tgatatttgg gagtgattat aatgcaaact aagatattga taatagatgg agacaaggat
     3061 aattgccaga agctaaaagg atttctggag gaaaaaggaa taagtataga tttagcatat
     3121 aattgtgaag aagctattgg aaaaatattt agcaataaat atgatttaat atttttagaa
     3181 ataattctaa gtgatggtga tggatggact ctttgtaaaa aaattagaaa tgtaactact
     3241 tgtccaattg tttatatgac ttatataaat gaagaccaaa gcattttaaa tgctttaaat
     3301 tctggtggag atgattactt aataaagcct ctaaatttag aaatattata tgcaaaagta
     3361 aaagctattt taagaagaat gaactcatat gtaaataata atgaaaataa tacaaaatta
     3421 gaggaatata atagaactag tggagtaata aagttagaag ataaaataat aaaattgact
     3481 ccaacagaaa ataagttgtt aaactttttt atagagaatc ctgaaaagac tttgactact
     3541 aaagagatat atgaacatgt atggatgaat gaatacttag aggataacta tagtgtagta
     3601 gttgcagtga atggattaag aaagaaaata gagaaagatt ataaaaatcc tcagaaaatt
     3661 ataacaataa gaggagcagg atattatttt aataaggaat aataaacata ttaatatcat
     3721 taataattat tataaatgtg aaatgctaac gatatctaaa ctaaattatt ataaaatagc
     3781 tgactgtaat ttaattgata
//
LOCUS       DAEPXF010000268         3720 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain HB27
            SAMN13376389-rid12479423.denovo.276, whole genome shotgun sequence.
ACCESSION   DAEPXF010000268 DAEPXF010000000
VERSION     DAEPXF010000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13376389
            Sequence Read Archive: SRR10518432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3720)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3720)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001069624.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001069624.1
            Genome Coverage       :: 315x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/15/2021 01:54:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,165
            CDSs (total)                      :: 4,076
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 89
            rRNAs                             :: 10, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 10 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 19 of 69
            Pseudo Genes (incomplete)         :: 44 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 10 of 69
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3720
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13376389-rid12479423.denovo.276"
                     /strain="HB27"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="China: Hebei"
                     /collection_date="2017"
     gene            <1..895
                     /locus_tag="KRL67_003675"
     CDS             <1..895
                     /locus_tag="KRL67_003675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBH1524509.1"
                     /translation="IELIDKDINIEMYYKNGNSYIEDRTSVDIIRESDFKKLRQYQNN
                     NYEELTSKESVYYVSDSYKKIFFKDFEIEKINLHLKDTNEYDINLFKVQKSILEPEIN
                     SQATFDLIVVKDDVFNQLKLHENSRLIRLIDIKNEKKELDLTLKLKNIVSENMKFTYP
                     FNFTSSIESYNNLIKLSGLMLFIGIFLSVVFLLCTGSIILFKQLSNIYDDKERYIMLT
                     KLGANNKDIEKIISKQLKVIFLMPLVVGTVHNLFAMSIAQKFIPRSLLVPIIITLVIY
                     FIGYFIYYFLTLKYALNMIKE"
     gene            1120..2733
                     /gene="ggt"
                     /locus_tag="KRL67_003676"
     CDS             1120..2733
                     /gene="ggt"
                     /locus_tag="KRL67_003676"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HBH1524510.1"
                     /translation="MKFNAYQHKYSSIRNVVYAKNGAVATSTPLASQAGLEILKKGGN
                     AVDAAVATAATLAVVEPTSNGIGGDAYALVWIEEEKRLYGLNSSGFAPENMELKNYNN
                     MKEMPKYGFGAVTVPGIPAAWSELNKKYGKLSLMECLSPAINYAREGYVVSPNVAKVW
                     KKSYELYEKELIGEEFKPWFDTFSKDGKAPEAGDIFICEEQASTLEEIANTQAESFYR
                     GRLADKIDEYSKKFNGAIRKSDLECFYPTWVEPISTEYKGYKIFEIPPNGHGITVLMA
                     LNILKELELEGNIENVEDIHKIIESLKLAFADSKTYVTDIEHMKVKIQELLSQEYAKK
                     RSLLIDNKEALYPTAGEPYCGGTVYLCTADKGGNMVSYIQSNYINFGSGIVIPRTGIA
                     LHSRGNNFNLDPKHHNVVKPFKKPYHTIIPGFLGKEDKAIGPFGVMGAFMQPQGHIQV
                     LTNMIDFGLNPQEALDAPRWQWIKGKEIEVEPEMPKHIIDSLIEKGHEIKVIHDTVDM
                     GRGQIIFKTEQESYICGTESRCDGHIAVY"
     gene            complement(2866..3717)
                     /locus_tag="KRL67_003677"
     CDS             complement(2866..3717)
                     /locus_tag="KRL67_003677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase"
                     /protein_id="HBH1524511.1"
                     /translation="MKNIISAKELISKLEKNDNLVVIDCRFDLINRTYGIDSYKKGHI
                     KGSFILDIDKDLSSPTKEHGGKNPLQDPLILKEKLEKMGVDNDTTIVTYDDGDLNGAC
                     RLFFQLKHLGLKNVYVLDGGITSFVKEGGELEEKVNIPSCTGKEIRPNINNDLVVPME
                     YVKSKLYKKDTVIIDCRANERYQGLVEPAYSKAGHIPSAKNYFCKDLIKSNFENGSLK
                     DIEFLKKFFKDLNNYDEVILSCGSGISACVNSLALRELDIPHKIYIGSFSDWISYDDN
                     EIKTGQE"
BASE COUNT         1448 a          463 c          591 g         1218 t
ORIGIN      
        1 aatagaactt atagacaaag atataaatat tgaaatgtat tataaaaatg gaaatagtta
       61 tatagaagat agaacttctg tagatattat tagagaaagt gattttaaaa aattaagaca
      121 atatcagaat aacaattatg aagaattaac ttcaaaggaa tctgtgtact atgtttctga
      181 ttcatacaaa aaaatatttt ttaaagactt tgagattgaa aaaataaact tgcatcttaa
      241 agatacaaat gagtatgata taaatctgtt taaagtacaa aaaagcatct tggagccaga
      301 aataaattca caagctacgt ttgatttaat tgtagtaaaa gatgacgtat ttaatcagtt
      361 aaaattacat gaaaattcta gattaataag attaattgat ataaaaaatg aaaaaaaaga
      421 actggatttg actttaaaat taaaaaatat agtaagtgaa aatatgaaat ttacttatcc
      481 ttttaatttt acatcaagta ttgaaagtta taataacttg ataaaattaa gtggtctaat
      541 gctatttata ggcatatttt tgtctgtagt atttctttta tgtacaggca gtataatctt
      601 atttaaacaa ttgtcaaata tatatgatga taaggaaaga tatattatgt taacaaaact
      661 tggagcaaat aataaagata ttgagaaaat aatatcaaaa cagttaaaag ttatattttt
      721 aatgccatta gttgtaggta cagttcataa tttatttgct atgtctatag cacaaaagtt
      781 tataccaagg tcattactcg taccaattat cataactcta gtcatttatt ttataggata
      841 ttttatatat tattttttaa ctttaaaata tgctctaaac atgataaaag aataaattat
      901 ttttaagtta aaagaattag atatgtgttt tgaataaaat aacttattta agtgcagata
      961 gaaaatacta gaattgagtt taagatatct atttaaagat aaaattaaag ttaggaaaaa
     1021 gtctatattt tataccattt tatatcagtt acattacatg atgttatata ataaatctaa
     1081 gcagaatatt aaataaaaaa tgacgaggag ctgaaattta tgaagtttaa tgcctaccaa
     1141 cataaatact catcaataag aaatgtcgtg tatgctaaaa atggagcagt tgcaacatca
     1201 acaccattag catctcaagc tggacttgaa atactaaaaa agggtggaaa tgctgttgat
     1261 gcagcagttg caacagccgc cacattagca gtagtagaac ctacaagcaa tgggattgga
     1321 ggagatgcat atgcccttgt ttggatagag gaagaaaaac gattatatgg tttaaattct
     1381 agtggctttg ctccagaaaa tatggaatta aaaaattata ataatatgaa agaaatgcca
     1441 aaatatgggt ttggagcagt aactgtacct gggatacccg cagcttggtc agaattaaat
     1501 aaaaaatatg gtaaacttag cttaatggaa tgtttatctc cagcgataaa ctatgcaaga
     1561 gagggctacg tagtatctcc taatgtagct aaagtatgga aaaaatcata tgaactatat
     1621 gaaaaagaac ttattggaga agagttcaaa ccatggtttg atacattttc taaagatggt
     1681 aaagctccag aggctggaga tatatttata tgtgaagaac aggcaagtac attagaagag
     1741 atagcaaata ctcaggctga aagtttttat agaggaagac ttgctgataa aatagatgaa
     1801 tattctaaaa aatttaatgg agctataaga aagagtgact tagagtgttt ttatccaaca
     1861 tgggtagaac ctataagtac agaatataag ggatataaaa tatttgaaat cccaccaaat
     1921 gggcatggaa taaccgtttt aatggcactt aatatattaa aagaactgga attagaagga
     1981 aatatagaaa atgtagaaga catacataaa attatagaga gtttaaaatt agcttttgca
     2041 gattctaaaa catatgtaac tgatatagaa catatgaagg taaagattca ggaattacta
     2101 agccaagagt atgctaaaaa aagaagttta ttaattgaca ataaagaggc tttgtatcca
     2161 actgcaggag agccatattg tggtggtaca gtataccttt gtactgcaga taagggtgga
     2221 aatatggttt catatataca gagcaattac ataaattttg gttcaggaat tgtgatacca
     2281 agaactggga ttgctttaca tagtagaggt aataatttta atttagaccc taaacaccac
     2341 aatgtagtaa aaccatttaa aaaaccttat catactataa tacctggatt tttaggtaaa
     2401 gaagataaag caataggacc atttggtgta atgggagctt ttatgcaacc acaaggacat
     2461 attcaagtgt taacaaatat gattgatttt ggattaaatc cacaagaggc gctagatgct
     2521 ccaagatggc agtggataaa aggaaaggaa atagaagttg aaccagagat gccaaaacat
     2581 ataatagatt cattaattga aaagggtcat gaaataaaag taattcatga tactgtcgac
     2641 atgggacgag gtcaaataat atttaaaaca gaacaggaaa gttatatttg tgggacggag
     2701 tcaagatgtg atggtcatat tgcagtttat taatattatc aactaaaata tatataataa
     2761 atttaaagag tatatgaaat aataagtaaa ggcgaaagtc tacaatgact gacgccttta
     2821 caaaatttaa gctatcatat gctgatttta tatagattat cagccttatt cttgaccagt
     2881 tttgatttca ttgtcatcat aactaatcca gtcactaaag ctaccaatat aaatcttatg
     2941 aggtatatcc agttctctaa gagcaagact gttaacacaa gcagatatcc ctgaaccaca
     3001 agataaaatt acttcatcat aattatttaa atctttaaag aattttttta aaaattctat
     3061 atctttaagt gagccatttt caaagttact ttttattaaa tccttacaaa aataattttt
     3121 tgcacttggt atatgaccag cttttgagta agcaggttca actaaacctt ggtatctttc
     3181 attagcacgg caatctatta ttacagtatc ttttttgtat aatttagact taacatattc
     3241 cattggaaca actaaatcat tgtttatatt tggcctaatt tccttacctg tacaacttgg
     3301 tatatttact ttctcttcca attctccacc ttcctttacg aaagatgtta ttcccccatc
     3361 tagtacatag acatttttaa gtcccaaatg ttttaattgg aaaaataatc tgcaagctcc
     3421 atttaaatct ccatcatcat aagttacaat tgtagtgtca ttatcaacac ccattttttc
     3481 taatttttct tttagtatta aagggtcttg taaaggattt ttaccaccat gttcttttgt
     3541 aggagatgat aaatccttgt ctatatctaa gataaaactg cctttgatgt gtcctttttt
     3601 ataagagtca ataccataag ttctattgat aagatcaaat ctacaatcta ttacaactag
     3661 attatcattt ttttctagtt ttgatataag ttcttttgct gaaataatgt ttttcaaaga
//
LOCUS       DAEPXF010000269         3714 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain HB27
            SAMN13376389-rid12479423.denovo.277, whole genome shotgun sequence.
ACCESSION   DAEPXF010000269 DAEPXF010000000
VERSION     DAEPXF010000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13376389
            Sequence Read Archive: SRR10518432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3714)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3714)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001069624.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001069624.1
            Genome Coverage       :: 315x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/15/2021 01:54:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,165
            CDSs (total)                      :: 4,076
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 89
            rRNAs                             :: 10, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 10 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 19 of 69
            Pseudo Genes (incomplete)         :: 44 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 10 of 69
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3714
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13376389-rid12479423.denovo.277"
                     /strain="HB27"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="China: Hebei"
                     /collection_date="2017"
     gene            complement(148..717)
                     /locus_tag="KRL67_003678"
     CDS             complement(148..717)
                     /locus_tag="KRL67_003678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate propanoyltransferase"
                     /protein_id="HBH1524512.1"
                     /translation="MKLPIALSNKHVHLSQADIDVLFGAGHELTKFKPLSQPGQYACE
                     EKVDLVGPKGTLKGVRVLGPARAHTQVEISLADGFPLGVKAPIKESGDIAGTPGVKLV
                     GPAGEVEMKEGVIVASRHIHMSNEDAAKFGVTDKQIVKVKTSGPRALVFENVLVRASD
                     SFNLEMHVDMEEGNAAGVKNGDLVELIVD"
     gene            complement(789..1397)
                     /locus_tag="KRL67_003679"
     CDS             complement(789..1397)
                     /locus_tag="KRL67_003679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HBH1524513.1"
                     /translation="MDSNDKKGLKSPEIIIINGSPRINKNCSFIANEVKAMLEECGLS
                     CKIFNIYNMDIEYCTACGFCEKTGYCKFNDDMKPLYKIFDESKGTIVISPVHFDSISA
                     KLKTVVDRTQAIYASKYVLNKPSIDRNKKRIGMYIAVGGSKSYDSQFKGGQIVMDFFF
                     KSINTKLLHNIYLNNTDDVSYIDNQEFKDIIYKNLKIYIDSI"
     gene            complement(1405..3168)
                     /locus_tag="KRL67_003680"
     CDS             complement(1405..3168)
                     /locus_tag="KRL67_003680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HBH1524514.1"
                     /translation="MTNGSYKMKNLVEISAMVTESTDFFNIKDDIIEKMLEVVHPTKA
                     CVNLFYKNDSKYAYLVCSQTLEYVPQIFPINSLKGAKIDFDTYPEYIHEAVNEKKIIY
                     VKNVFEDSRAEGERDLAKAEGYIGRIVFPFIINNVVVGFMTCFLKEDDYLTEQDIDFI
                     SSVASLLSLSIEITNKNNNVQILIDKLRGSISAINEATKKLYLNKSIDGFLENLSKQA
                     CNITNSKEALIIINSHHNKDTIVSCYNVEQKKKTNLYPMIDMFLKKESLGGYLNNLKP
                     CSKKGINLESYIYYKLQDKNETIGCIVCANSKSYTSDDLNILSILSKQVSVGMQLYEH
                     NEKEVKHKVLENELNILNKQQQLIMDKGKMDCNDEKELYFYHKPARVVGGDFYYATKV
                     DDEHIVYIVADVMGHGMVSNYVVAMIKGAFKVLCNQYNTPREIMTNLNRMLYDEFDKM
                     GVFTTCLIGIIDTKENMITVSNAGHYSPVVVKKDGTIENNLNCKKGIPIGIMEDATYE
                     NNTLSMEDYAMVCMYTDGILEIKNSSKEEYGINRLENFLKENFRLNQQLIVENLKLDL
                     KNFSSKDNYDDDILIVMLKDR"
     gene            complement(3211..3678)
                     /locus_tag="KRL67_003681"
     CDS             complement(3211..3678)
                     /locus_tag="KRL67_003681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH1524515.1"
                     /translation="MKMLNGIIKLFYSEIVKTKINNLDSSGYSLRDECKEIRNITLKI
                     DSVFFKLLKLAFGSILLMAAFNLNMVLGIGVFLVGVVYILYKRNMEEQVKEHINNVKN
                     NIDISKITFMDEKGRTGLNALITLLLIGLLTGFNWILVASFVVVFVFTIKNVC"
BASE COUNT         1206 a          667 c          340 g         1501 t
ORIGIN      
        1 tttttgtaag tttttattac attttttata ataccccttt attttataaa ttaatctaat
       61 cgattaaatt tatcctatag aaatttatta catttaaaaa tatgctagca aataaaatat
      121 ctactagcat aaagtctatt tataatatta atcaactatt aattctacta aatctccatt
      181 tttaacgcct gctgcatttc cctcttccat atcaacatgc atttctagat taaagctatc
      241 actagctctt actaatacat tttcaaatac taatgctctt ggtccagatg ttttaacttt
      301 tactatttgt ttatcagtta caccaaattt agcagcatct tcattgctca tatgtatatg
      361 tctagaagca actataactc cttctttcat ttcaacttct ccagcaggtc ctactaattt
      421 aacacctgga gttccagcta tatcaccaga ttcttttatt ggtgctttta ctcctaatgg
      481 gaaaccatct gccaaagata tttcaacttg agtatgagct ctagctggtc ctagtactct
      541 aactcctttt aaagttcctt ttggtcctac taaatccact ttttcttcac aggcatattg
      601 ccctggttga gataatggtt taaatttagt taactcatga cctgccccaa ataatacgtc
      661 tatatcagct tgacttaaat gaacatgttt attagataat gctattggta atttcattga
      721 aattccctcc ttttaattat ttaaattatt gcaatacaat tatacataat attacctaca
      781 aaatcaaatt atattgaatc aatatatatc tttaaatttt tatatattat atctttaaat
      841 tcttgattat ctatataaga tacatcatct gtattgttta aatatatgtt gtgtaaaagt
      901 ttagtattta tactcttaaa gaaaaaatcc ataacaatct gtccaccttt aaactgtgaa
      961 tcatatgatt tactcccccc cactgcaata tacattccta tccgtttctt atttctatca
     1021 attgaaggtt tatttaatac atatttactt gcatatatag cttgagttct atcaacaaca
     1081 gtctttaatt tagctgatat tgaatcaaaa tgtacaggac ttattactat agttcctttt
     1141 gactcatcaa atattttata taatggcttc atatcatcat taaacttaca ataccctgtt
     1201 ttttcacaaa atccacatgc tgtgcaatat tcaatatcca tattgtagat attaaatatt
     1261 ttacaactta atccacactc ttctagcata gcttttactt catttgctat aaaactgcaa
     1321 tttttattaa ttcttggact tccatttatt attataatct ctggactttt aagtcctttt
     1381 ttatcgtttg aatccataat caatctacct atcctttaac ataactatta aaatatcatc
     1441 atcataatta tcttttgatg agaagttctt taaatccaat tttagatttt ctactattaa
     1501 ttgttgattt aatctaaagt tttcttttag aaagttttct aatcggttta taccatactc
     1561 ttcttttgat gaatttttta tctctaaaat tccatcagtg tacatacaca ccattgcata
     1621 atcttccatt gaaagagtat tattttcata agtagcatct tccattattc caataggtat
     1681 gcccttttta caatttaaat tattttctat tgtcccatct ttctttacaa ctacaggact
     1741 ataatgacct gcattagaca cagtaatcat attttcttta gtatctataa tacctatcaa
     1801 acaagttgta aatactccca tcttgtcaaa ttcatcatat aacatcctat ttaaatttgt
     1861 cattatttct cttggcgtat tgtattgatt gcataatact ttaaaagcac cttttatcat
     1921 tgcaacaaca taatttgata ccattccatg tcccattaca tcagcaacaa tataaactat
     1981 atgttcgtca tccacttttg tagcataata aaaatcccca cctactaccc ttgctggttt
     2041 atggtaaaaa taaagttctt tctcatcatt acaatccatt ttacctttat ccataataag
     2101 ctgttgttgt ttatttaata tgttcaactc attttcaaga actttgtgtt ttacttcctt
     2161 ttcattatgt tcatataatt gcatcccaac agaaacttgt ttggacaata tactaagaat
     2221 atttaaatca tcactagtat agcttttact atttgcacaa actatacatc ctatagtctc
     2281 atttttatcc tgaagtttgt aatatatata actttccaaa tttattccct tttttgagca
     2341 tggttttaaa ttatttaggt acccccccaa tgactctttc tttaaaaaca tatcaatcat
     2401 aggatataaa tttgttttct ttttttgctc aacgttataa caactaacta tagtatcctt
     2461 attatgatga ctatttataa ttattaaagc ttctttacta ttagtaatat tgcacgcttg
     2521 tttacttaaa ttttctaaaa agccatcaat actcttatta aggtatagtt ttttagtagc
     2581 ttcatttatg gcacttattg agcctcttag tttatcaatt aaaatttgta cattattatt
     2641 tttattagtt atttctattg ataaacttaa gagtgatgca acagatgata taaaatctat
     2701 atcttgttct gttagataat catcctcttt taaaaaacaa gtcataaatc caacaactac
     2761 gttatttatt ataaaaggaa aaactattct cccaatataa ccttcagctt ttgctaaatc
     2821 tcgttcacct tctgctctag aatcttcaaa tacatttttc acatatatta tctttttttc
     2881 atttacagcc tcatgtatat actctggata cgtgtcaaaa tcaatttttg cacctttaag
     2941 actatttatt ggaaaaattt gaggtacata ttctaaggtt tgcgaacata ctaagtaggc
     3001 atatttagaa tcatttttat aaaatagatt cacacatgcc tttgtagggt gaacaacttc
     3061 taacattttt tctattatgt catcttttat attaaaaaaa tctgtagatt cagtcaccat
     3121 agcggatatc tccactaaat tcttcatttt ataactgcca ttagtcatac tcaatttctc
     3181 cccctatata ttaaactatt tactagtatt ttaacataca ttctttatag taaaaacaaa
     3241 aacaacgaca aaactagcaa ctaatatcca gttaaatcct gtcaataatc ctataagaag
     3301 taatgtaatt aatgcattta gacctgttct ccctttttca tccataaaag ttattttaga
     3361 gatgtcgata ttatttttca cattatttat atgctctttt acttgttctt ccatatttct
     3421 tttatatagt atataaacta caccaactaa aaacactcct atacctagaa ccatgtttaa
     3481 attaaaagct gccataagca atattgaacc aaaagctaac tttaaaagct taaaaaatac
     3541 gctatctatc tttagagtta tatttctgat ttctttacat tcatctctta aagaatatcc
     3601 acttgaatct aagttattaa tctttgtttt tacaatttca gaataaaaaa gctttattat
     3661 accatttaac attttcatca ctcctaaata ttttacatat atttgtaagt ttta
//
LOCUS       DAEPXF010000270         3713 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain HB27
            SAMN13376389-rid12479423.denovo.278, whole genome shotgun sequence.
ACCESSION   DAEPXF010000270 DAEPXF010000000
VERSION     DAEPXF010000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13376389
            Sequence Read Archive: SRR10518432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3713)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3713)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001069624.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001069624.1
            Genome Coverage       :: 315x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/15/2021 01:54:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,165
            CDSs (total)                      :: 4,076
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 89
            rRNAs                             :: 10, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 10 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 19 of 69
            Pseudo Genes (incomplete)         :: 44 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 10 of 69
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3713
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13376389-rid12479423.denovo.278"
                     /strain="HB27"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="China: Hebei"
                     /collection_date="2017"
     gene            237..1817
                     /locus_tag="KRL67_003682"
     CDS             237..1817
                     /locus_tag="KRL67_003682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional ADP-dependent NAD(P)H-hydrate
                     dehydratase/NAD(P)H-hydrate epimerase"
                     /protein_id="HBH1524516.1"
                     /translation="MLIGTSKLSRNVDTACVEEVGIPMIVLMENAVISAMKNMDIDMY
                     NSYTVVCGVGNNGGDGLGIARHLNTYGKEVNVFLVGNIEKLSECSKINYNVLLNMGIN
                     IININKNDLSEKNYGDENEVDKIIKVEKQNIIKNKNLTIFKDRVSKSDVVVDAIFGTG
                     LKRDIAGIFKEVIDIVNDNSKNTYSIDIPSGIDSDNGNVLGTCIRANKTISFEFYKRG
                     FLNYDTSALIGDVVVEKIGVPDFITRKYHDEEFITDKTFVKNNIRRRDKHGFKSDFGK
                     VSIVAGSKGFYGASFIATESAVKSGSGLVTLISSEDVQEKVSIRLTEAMTLNFEEEGL
                     NKLLNSSDAIGFGPGMGNNSQTFGKLLKIVENSNCPIVLDADGLNVMKDRCYKFLEWK
                     NKFVITPHLGEMARLTGDSIGYIREHRVDVAKEFAQKYNVVVLLKGYQTVITDGKKTY
                     INPTGNSCMATGGMGDCLLGMITSFIGQGMDILEATVSGAYIHGYIGDKLSKAMYTVN
                     ATDLISNISLTMKELLDI"
     gene            1991..2437
                     /locus_tag="KRL67_003683"
     CDS             1991..2437
                     /locus_tag="KRL67_003683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HBH1524517.1"
                     /translation="MKLIWLIVAVLFGIAEMLTPSLTLIWFSVGAVILIFLSSFIESI
                     FLQILIFAVISIAMLVVATKKIVKKDKGYKSNTNLQAMMSKKGIVTEEISPNNTGLVV
                     VEHETWTAISIDGEKIEKGSTVEVLKIEGVKLVVKKVNATTSVTNQ"
     gene            2458..3501
                     /locus_tag="KRL67_003684"
     CDS             2458..3501
                     /locus_tag="KRL67_003684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH/Band 7/PHB domain protein"
                     /protein_id="HBH1524518.1"
                     /translation="MGIKIVLSIVLIVVVIAISLTCIRVIKQSKVGIIMRLGKFQKVA
                     ETGVHFLIPFLDKMAYVIDLREIVIDFPPQPVITKDNVTMQIDTVVYYKVTDPVRYVF
                     EIANPIAAIENLTATTLRNIIGELDLDETLTSRDIINVKMRTILDEATDKWGIKVNRV
                     ELKNIMPPHDIQVAMEKQMRAERERREAILQAEGNKSAAILQAEGEKQSAILTAEAKK
                     EAMVRVAEGEKESAILVAEGEAEAIRQTAIAKAQGEAEMIKRTQIATAEGLKLVFSAM
                     KEADIDNNILALKSMEALEKMAEGKSTKLVLPSEAVNFLGTFKGIKEVMSDDNKEVLD
                     IKEVLNDNESLKK"
BASE COUNT         1500 a          338 c          699 g         1176 t
ORIGIN      
        1 aaagagatat ttagataatt agtgtaaata ggttttaaat caacataata acttaaatta
       61 tatggtataa tgaattaaat aattgtttta attcaaaatt ttttgttatt ttaaattatt
      121 tcaaaaatct tagttttagt gattttattt atgatattta tgggattaga aagatttcaa
      181 tagatatttt ttggtaagta tttaagaagt ctttaacttt ataggagtga taatttatgc
      241 taataggtac atctaaatta agcagaaatg ttgatactgc atgtgtagaa gaagtaggga
      301 ttccaatgat tgtattgatg gaaaatgcag ttatatctgc aatgaaaaat atggatatag
      361 acatgtataa tagttataca gttgtatgtg gtgttggaaa taatggagga gatggtcttg
      421 gaatagcaag acatctaaat acatatggaa aagaagtaaa cgtattttta gtaggtaata
      481 ttgaaaaact aagtgaatgt tctaaaataa attacaatgt acttttaaat atgggaatta
      541 atattataaa tattaataaa aatgatttaa gtgaaaaaaa ttatggagat gaaaatgaag
      601 tagataaaat aattaaagtt gagaaacaaa atattataaa aaataaaaat ttaacaattt
      661 ttaaagatag agtatctaaa agtgatgttg tagtagatgc tatatttggg actggactaa
      721 aacgtgatat agctggtatt tttaaagaag ttattgacat agtaaacgat aatagcaaaa
      781 atacatattc tatagatatt ccttcaggta tagatagcga taatggaaat gtattaggaa
      841 cttgtattag agcaaataaa acgattagct ttgagtttta taaaagaggt tttctaaact
      901 atgatactag cgcacttata ggtgatgtag ttgtagaaaa gataggtgtt ccagatttta
      961 ttacaagaaa ataccatgat gaagagttca taacggataa gacttttgta aaaaataata
     1021 ttagaagaag agataagcat ggttttaaaa gtgattttgg aaaagtatct atagttgcag
     1081 gttcaaaagg attttatggt gcaagtttca tagcaactga atctgctgtt aagagtggaa
     1141 gtggacttgt aacattaatt agtagtgaag atgtgcaaga aaaggtatct attagactta
     1201 cagaagcaat gactttaaat tttgaagaag aaggattaaa taaattactt aattctagtg
     1261 atgcaattgg ttttggtcca ggtatgggaa ataatagtca aacatttggt aaattactta
     1321 agatagtaga aaattctaat tgcccaatag tattagatgc tgatggattg aacgttatga
     1381 aggacagatg ttataagttt ttagaatgga aaaataaatt tgtaattaca cctcatttgg
     1441 gagaaatggc caggttgaca ggcgattcaa ttgggtatat aagagaacat agagtagatg
     1501 ttgcgaaaga atttgctcaa aaatataatg tggtggtgct tttaaaaggt tatcaaactg
     1561 tcataacaga tggtaaaaaa acatatataa atcctacagg aaatagctgt atggcaactg
     1621 gtggaatggg tgactgcttg cttggaatga ttacttcatt tataggtcaa ggtatggata
     1681 tattggaagc tacagttagt ggagcatata tacatggcta tataggagat aaattgagca
     1741 aagcaatgta tacggtaaat gctacggatt taataagtaa catttcatta acaatgaaag
     1801 agttgttgga tatataattg gtaatatttg atactacttt gtaacaattt taacaaagta
     1861 tgttgatata atactattaa ttaattaatt tattttattg ttttgtaatt taaatttata
     1921 ttcaaaaaca ttgtataaac atataattta tatagggttt tataattcat atgaaaagag
     1981 ggtagatagt atgaagttaa tatggttgat agtagcagtt ttatttggaa tagcagaaat
     2041 gttaacacca agcttaacat tgatatggtt tagtgtagga gctgtaatac ttatattttt
     2101 aagtagcttt atagagagta ttttcttaca aattttaata tttgcagtaa tttctatagc
     2161 aatgttagtc gtagctacta aaaaaatagt aaaaaaggat aaaggctaca aatctaacac
     2221 taatttacaa gcaatgatgt ctaaaaaagg tattgttaca gaagagattt ctccaaataa
     2281 cacaggtcta gttgtagttg aacatgaaac atggacggct atttctatag atggtgaaaa
     2341 gatagaaaaa ggttctacag tagaagtatt aaaaattgaa ggggtaaaac tagttgttaa
     2401 aaaagtaaac gccacaacta gtgtgacaaa tcaatagaat aaaggaggat ttatagtatg
     2461 ggtattaaaa ttgttttaag tattgtatta atagtagtag tcatagcaat aagtttaacc
     2521 tgtataagag ttataaaaca atctaaagta ggtataataa tgagacttgg taagtttcaa
     2581 aaagtggctg aaacaggtgt acatttctta ataccatttt tagataaaat ggcatatgta
     2641 attgacctta gagaaattgt aatagatttt ccaccccaac cagttataac taaagataat
     2701 gtaactatgc agattgatac agttgtatat tataaagtta cagacccagt tagatatgtg
     2761 tttgagatag ctaatccaat agctgctatt gaaaatttaa cagctacaac actaagaaat
     2821 ataattggtg aacttgattt agatgaaaca ttaacatcaa gagatataat aaatgtaaaa
     2881 atgagaacta tccttgatga agcaacagat aaatggggta taaaagtaaa tagagtagag
     2941 ttaaaaaata taatgcctcc tcatgatatt caagttgcga tggaaaaaca aatgagagca
     3001 gaaagagaaa gaagagaggc aatacttcaa gcagaaggta ataagtcagc tgcaatactt
     3061 caagcagagg gtgaaaaaca atctgcaata ttaacagcag aagctaaaaa agaagctatg
     3121 gtacgtgtag cagaaggtga aaaagaatct gctatattag tggcagaagg tgaagctgaa
     3181 gctataagac agacagctat tgctaaggca caaggtgaag ctgaaatgat aaaaagaact
     3241 cagatagcaa cagcagaagg tttaaaatta gttttttcag caatgaaaga agctgatata
     3301 gacaataata ttctagcatt aaaatctatg gaagctcttg aaaaaatggc tgaaggtaag
     3361 tcaacaaaac tggttttacc ttcagaagca gttaatttcc taggaacatt caaaggaata
     3421 aaggaagtta tgagtgatga taacaaagaa gtacttgata taaaagaagt tctaaatgat
     3481 aatgaatcat taaaaaaata aaagagtttt gttatcatag gaaggatatg gaaattaaag
     3541 gtcgaattaa ttaaatatag cacaaaaatg cacgttgaaa agaagaggac ttataaattt
     3601 ttaagtcttc ttttttgttt ttattagaaa gataaaatta taaaaataaa ttaacagaat
     3661 ctttatacaa tttatccttt actaatgtaa aaagtgttat ataatattat ata
//
LOCUS       DAEPXF010000271         3712 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain HB27
            SAMN13376389-rid12479423.denovo.279, whole genome shotgun sequence.
ACCESSION   DAEPXF010000271 DAEPXF010000000
VERSION     DAEPXF010000271.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13376389
            Sequence Read Archive: SRR10518432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3712)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3712)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001069624.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001069624.1
            Genome Coverage       :: 315x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/15/2021 01:54:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,165
            CDSs (total)                      :: 4,076
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 89
            rRNAs                             :: 10, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 10 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 19 of 69
            Pseudo Genes (incomplete)         :: 44 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 10 of 69
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3712
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13376389-rid12479423.denovo.279"
                     /strain="HB27"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="China: Hebei"
                     /collection_date="2017"
     gene            135..470
                     /locus_tag="KRL67_003685"
     CDS             135..470
                     /locus_tag="KRL67_003685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBH1524519.1"
                     /translation="MYNIDDMIYNCPVEALSNILGKKWVAIIIWKIQEDKKRFGELQR
                     AIPDCSKKMLVQQLDFLIEQGIIVNQKKLVNNIVESTYFLSESGLLLLPVMEKMIMWS
                     NKNLNCDKY"
     gene            607..1884
                     /locus_tag="KRL67_003686"
     CDS             607..1884
                     /locus_tag="KRL67_003686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin family protein"
                     /protein_id="HBH1524520.1"
                     /translation="MQNVNLFLVFIEGIVSFFSPCILPILPIYLSILSNSSVENLKEG
                     KTSFIGSSLFKNTVFFALGISTTFFILGSSVKVLSMFFNENKDLIMFIGGIIIIIMGL
                     FYMGIIKSSILNREKRFNLKFKEMRAITAFLLGFTFSFGWTPCIGPILASVLVMVSSS
                     SNHLSANLLIAVYTIGFILPFIITAMFYSKLFKTIDKIKSNMEIIKKIGGIILIVSGI
                     LMMVNGFGSISKHFNTSQNSKIESKQEDNKRENSTDKEENSDGNDSQKDSNNENNDKG
                     SNDEDRIKSIDFTLTDQYGKTHKLSDYEGKVVFLNFWATWCPPCKEEMPYIEQLYKDY
                     NKNNDDVVILGVASPNLGREGSREHVVNFLKGQSYTFPVVLDEDGALAYQYGINAFPT
                     TFIIDKEGYVTKYIPGAMDKATMKSFIENQRNK"
     gene            2005..3393
                     /locus_tag="KRL67_003687"
     CDS             2005..3393
                     /locus_tag="KRL67_003687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HBH1524521.1"
                     /translation="MKKTFDAIIIGFGKGGKTLAGDLANRGLKVALIEKSNKMYGGTC
                     VNVACIPTKSLENSANSVKTKNINSWDEVQAEYGKAIDKKETLITKLREANYNKLNSN
                     ENVTIFTGMGTFIDEKTVQVKTENEIYELVADNIFINTGSRPFIPNIKGIENKNIVYD
                     SESLMNLRVLPKKMTIIGAGFIGLEFAGIYSSFGAEVTILNSNNGILPNEDVEDSEEI
                     IKLLAKRNVKIVNNANIKEIKEVSKLAIVEYEVDGKSKELASNMILVATGRKANTEGL
                     GLENAGIELNKRGFIKVSETLKTNKEHIWAIGDINGGPQFTYISLDDYRIIINQLFGD
                     KTRTTNDRKNIPNSIFISPAFSRVGLNVKQAKEKGYEVLVAKMPVEAIPRAKQIGKAD
                     GFIKIVIDKKSNKILGASMICENSSEIIHLIQLAVDLEVEYTYLRERVYAHPTMTEAL
                     NDILSPNMIKEV"
     gene            complement(3506..>3712)
                     /locus_tag="KRL67_003688"
     CDS             complement(3506..>3712)
                     /locus_tag="KRL67_003688"
                     /inference="COORDINATES: protein motif:HMM:NF024729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HBH1524522.1"
                     /translation="MSRRGNCWDNAPIESFFSHFKSELIYLIDTTDPKEMISLINDYI
                     YFYNNERIQLKNGMSPIEYRTHSE"
BASE COUNT         1515 a          370 c          640 g         1187 t
ORIGIN      
        1 atctaaaata gtttgtaata ggagtatata ataaaaattt taattaaata aagctaggca
       61 tatttataat attaataaat atattgctat aaaagtttgt ttaataaatt aaataaagaa
      121 aagaggagaa taaaatgtac aacatagatg atatgatata taattgtccg gtagaagctt
      181 tatcaaatat tttaggtaaa aagtgggtgg ctataattat atggaaaata caagaagata
      241 aaaagcgatt tggggagtta caaagagcta taccagactg tagtaagaaa atgttagttc
      301 aacaactaga ttttttaatt gaacagggta ttatagtcaa ccaaaaaaag ttggttaata
      361 atattgtaga gtcaacatat tttttaagtg aatcaggatt actattgctt cccgtcatgg
      421 aaaaaatgat tatgtggagc aataaaaatt taaattgtga taaatactaa aatttaatct
      481 gtaagtaatg aaaatttaat taagctatga gtagaaaaat gtattaaaat tatataattt
      541 tttagttaga ttttaattta gaaatgggat tgtaatattg gaaataaaat aaggaggaat
      601 tttaatttgc aaaatgtaaa tttattttta gtatttatag aaggtatagt atcattcttt
      661 tcaccatgta tactgccaat tttgcctatt tatttgagta tattgtcaaa tagtagtgta
      721 gagaatctaa aagaaggaaa gacgagtttt ataggaagtt ctctatttaa aaatactgta
      781 ttttttgctc ttggaatctc gacaacattt tttatattgg gttcatcagt aaaagtattg
      841 agtatgtttt ttaatgaaaa taaagattta ataatgttta ttggtgggat tataattatt
      901 atcatgggat tattttacat gggaataatt aaatcttcaa tattgaatag agaaaaaaga
      961 tttaatctta aatttaaaga aatgagagca ataacagctt ttctattagg ttttacattt
     1021 agttttggat ggacaccttg tattggtcca atattggcat ctgtattagt aatggtttca
     1081 agttcaagta atcatcttag tgccaatctt ttaatagcag tatatactat tggatttatt
     1141 ttgccattca taattacagc tatgttttac agtaaactat ttaaaactat agataagata
     1201 aagtctaaca tggaaattat taaaaaaata ggtgggatta tacttatagt atcaggaata
     1261 ttaatgatgg taaatggatt tggaagtata agcaaacact ttaatacgtc acaaaatagt
     1321 aaaattgaaa gcaagcaaga agataataag cgagaaaatt ctactgataa agaagaaaat
     1381 tctgatggta atgatagtca aaaagactct aataatgaaa ataatgataa agggtctaat
     1441 gatgaagata gaataaagtc tattgatttt actttaacag accagtatgg taaaacacat
     1501 aaattaagtg attatgaggg caaagttgta ttcttaaatt tctgggctac atggtgtcct
     1561 ccatgtaaag aagaaatgcc ttatatagaa caactgtata aagactataa taaaaataat
     1621 gatgatgtag taattttagg agtagcttca cctaatttag gtagagaagg ttcaagggaa
     1681 catgtagtta acttcttaaa aggccaaagt tatacattcc ctgttgtact tgatgaagat
     1741 ggagcattag catatcaata tggaattaat gcttttccaa ctacgtttat aattgacaag
     1801 gaaggatatg taactaagta tattccagga gctatggata aggcaactat gaaatcattt
     1861 attgaaaatc agagaaataa ataaaagtat ttaagtataa gtttataaat caatttaagg
     1921 ttacaaaaaa gtgcctaatt gtaccaccca aataatcttg ttatagtaaa agtataaaca
     1981 atacaagaaa gggttgaaat ttatatgaaa aaaacatttg atgctataat aataggattt
     2041 ggaaaaggtg gaaaaacatt agcaggggat ttggcaaaca ggggattaaa agtagcttta
     2101 atagaaaagt caaacaagat gtatggtgga acatgtgtaa atgtggcatg tataccaaca
     2161 aaaagcttag aaaattcagc aaacagtgta aaaacaaaga atataaattc ttgggatgaa
     2221 gtacaagctg aatatgggaa agctatagat aaaaaagaaa ctttaatcac aaaactcaga
     2281 gaagctaact ataataaatt aaattcaaat gagaatgtaa ctatattcac aggaatggga
     2341 actttcattg atgaaaaaac agttcaagtc aaaactgaaa atgaaattta tgagttagta
     2401 gcagataata tatttattaa tactggttca agaccattta tacctaatat taagggaatt
     2461 gaaaataaga atatagtata tgatagtgag tctcttatga atctaagagt tttacctaaa
     2521 aaaatgacaa ttataggagc tggatttata ggtttagagt ttgcgggtat ttatagttca
     2581 tttggagcag aagttacaat attaaactct aataatggca ttttaccaaa tgaagatgtt
     2641 gaggattcag aagaaataat aaaattactt gcaaagagaa atgtaaaaat tgttaataat
     2701 gcaaatataa aagagattaa agaagtttct aaattagcaa tagtagaata tgaagtagat
     2761 ggtaaatcta aagaattagc aagtaatatg atacttgtag ctacaggaag aaaagcgaac
     2821 actgaagggc tagggctaga aaatgcaggt atagaattaa ataaaagagg atttattaaa
     2881 gtatcagaaa ctcttaagac taataaagag catatatggg ctataggaga tataaatgga
     2941 ggaccacaat ttacttatat ttcattggat gattatcgta taattattaa tcagttattt
     3001 ggagataaga ctagaacaac taatgataga aagaatatac caaattctat atttataagt
     3061 ccagcatttt caagagttgg tcttaatgtt aaacaagcta aagaaaaggg atatgaagta
     3121 ttagtggcaa agatgccagt agaagcaatt ccaagagcta aacagattgg caaggcagat
     3181 gggtttataa aaatagtgat agataaaaaa tccaataaga tacttggagc tagtatgata
     3241 tgtgaaaatt caagtgagat tatccatctt attcaattag ctgtagattt agaagtagag
     3301 tatacatatt taagagaacg tgtttatgca catccaacta tgacagaagc tcttaatgat
     3361 attctatctc ctaatatgat aaaagaagta taacataagt ttaatatata aacctaaaat
     3421 tatcattata aatagatttt agaaagagac tatctcaaaa ttggatatgt ccctgtcaag
     3481 tagacagact taaaaaaggc tatctttact cggaatgagt tcgatattca atcggactca
     3541 ttccgttttt taattgtatt ctttcattat tataaaaata aatataatca tttattagac
     3601 taatcatttc ctttggatct gtagtatcaa ttaaatatat taattcagat ttaaaatgac
     3661 tgaaaaaact ttctatagga gcattatccc agcagttgcc ccttcgggac at
//
LOCUS       DAEPXF010000272         3702 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain HB27
            SAMN13376389-rid12479423.denovo.280, whole genome shotgun sequence.
ACCESSION   DAEPXF010000272 DAEPXF010000000
VERSION     DAEPXF010000272.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13376389
            Sequence Read Archive: SRR10518432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3702)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3702)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001069624.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001069624.1
            Genome Coverage       :: 315x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/15/2021 01:54:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,165
            CDSs (total)                      :: 4,076
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 89
            rRNAs                             :: 10, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 10 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 19 of 69
            Pseudo Genes (incomplete)         :: 44 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 10 of 69
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3702
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13376389-rid12479423.denovo.280"
                     /strain="HB27"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="China: Hebei"
                     /collection_date="2017"
     gene            108..542
                     /locus_tag="KRL67_003689"
     CDS             108..542
                     /locus_tag="KRL67_003689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH1524523.1"
                     /translation="MKILKKITSIIFLIIMITVVITGCSQQEQGKNNSEKKLNYNVLG
                     EKTRHGGTYGDFKFVVIEMPKDFDPSNCKNLSKDFLNYYNENVDTITVYVYEKGYKLE
                     SEQDMYKNSLYDITIEHRDENTCVYSVWDVKSEETTDEGSLN"
     gene            complement(630..2189)
                     /locus_tag="KRL67_003690"
     CDS             complement(630..2189)
                     /locus_tag="KRL67_003690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding repeat-containing protein"
                     /protein_id="HBH1524524.1"
                     /translation="MKSKKLLSLGLAMSMFVACYPISANALDKIDKIQGADKYETAGL
                     IADKQSYATAILINADSTMADGLSASGLAGATNAPILLTKKNNIPNATLKRVEKANKV
                     YIIGGESSIDKATETFLKDKGIETKRLQGNDRIKTSYNVAKEINSINKVNKVILTNAF
                     KGEPDAMSIASVAVRDKAPIILTDGKSVPFNTTGIESYAIGGTSSMSDKLVNDTNSTR
                     LGGADRYDTNKKIVNKLYNGIKEFYIASGTDLVYALVGSTIAKNTPIVLVDDNSNKTV
                     LKGATKITAIGNLNNNAIEQSLNITKNIASPTTGGKYTVKEATERIAYILDEKVFVDY
                     ESAIKAKTDPDECFRYYIKNFTYQGKDYYAIGADDDDFRFLVNMNNLNDILQWYSNNT
                     IKRFDAEGYLKERKAIDMAFEACSSKYDISENDLILDREEHNISTDFYVVVLYESSGI
                     DSYWVFIDTDTWTVHDIQRLNQNEELVSYHDVNPNTEADKHYNRDVDDVNIIFVPSEN
                     NPFDNDVDGIS"
     gene            2394..2834
                     /locus_tag="KRL67_003691"
     CDS             2394..2834
                     /locus_tag="KRL67_003691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Arm DNA-binding domain-containing protein"
                     /protein_id="HBH1524525.1"
                     /translation="MRGVIIIKGGVRKKGKKWYYYFDLGVIDGKRKKVERAGGNTKKD
                     AEKALREALKEYENTGIMFDECEMNLAEYLDFWFNKYVILNCKYNTQESYRIHIQTHI
                     KPALGHYKLKSLTPATLQNFINAKFRSNYSQSTLEVIRAILKKL"
     gene            2876..3535
                     /locus_tag="KRL67_003692"
     CDS             2876..3535
                     /locus_tag="KRL67_003692"
                     /inference="COORDINATES: protein motif:HMM:NF012798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HBH1524526.1"
                     /translation="MQYIAIPKNKTIKKEIKVITLDEFNNILNLFNKGTYQYISLMVA
                     FHTGMRKGEVAGLTWEDINLKTKTIDLYPPMIKKKKGDIELATPKSSSSYRTITIGDT
                     LVKALKEHKKYQNENKLLFGEFYINNNFVCTKINGSPININSISTMCNGIIKKINTHF
                     TFHSLRHTHATMLLEAGANIKAIQKRLGHSNIAVTMNTYSHVTETMKQETVDLFEKSI
                     K"
BASE COUNT         1351 a          544 c          475 g         1332 t
ORIGIN      
        1 taattgcata tttttccaaa acacatagca tctttgtaga aaattttata tacttacatt
       61 attactatca taaagtagaa taaaaatatg tgagggaggt ttattgaatg aaaatactaa
      121 aaaaaataac tagtataatt tttttaataa taatgataac agttgttatt acaggatgta
      181 gccaacaaga acaggggaaa aataattcag aaaagaaatt aaattacaat gttttaggag
      241 aaaaaacaag acatggtggt acatatggcg attttaagtt tgttgttata gaaatgccta
      301 aagactttga cccatcaaat tgcaaaaatt tgtctaagga ttttttaaac tactataatg
      361 aaaatgttga tacaataact gtttatgtat acgaaaaagg ttacaaatta gaaagcgaac
      421 aagatatgta taaaaattct ttatatgata taactattga gcacagagat gaaaatactt
      481 gtgtttattc tgtttgggat gttaaatctg aggagacaac agatgaaggt tcattaaatt
      541 aaaaccttta acttgtcttt ttagaaataa aagaggaact aagttatatt catttttata
      601 acttagctcc ttatagtaaa tatttatttt taacttattc catctacatc attatcaaaa
      661 ggattatttt ctgatggaac gaatataata ttaacgtcat caacatctct attataatgc
      721 ttatctgctt ctgtatttgg gtttacatca tgatatgata ctaattcttc attttgattt
      781 aaacgctgta tatcatgaac agtccatgta tcagtatcta taaaaaccca atatgaatct
      841 attccagaac tctcatacaa tacaactaca taaaaatcag tacttatatt atgttcttct
      901 ctatctagta tcaaatcatt ttcactaata tcatatttac tagaacatgc ttcaaatgcc
      961 atatcgattg cttttctctc ttttaaatat ccttccgcat caaacctttt tattgtatta
     1021 tttgaatacc attgtagtat atcattcaaa ttattcatat ttactaaaaa tctaaaatca
     1081 tcatcatctg caccaattgc ataataatct ttcccttgat aggtaaagtt ttttatataa
     1141 tacctaaaac attcatccgg gtctgttttt gctttaatag ctgattcata atctacaaat
     1201 actttttcat ctaaaatata agctattctt tcagttgctt cctttacagt atacttccct
     1261 ccagtagttg gactggcaat attttttgtt atgtttagag attgttctat agcattgtta
     1321 tttagatttc ctattgctgt tattttagtt gcacctttta atacagtctt attactattg
     1381 tcatcaacta acacaattgg agtattttta gctattgtag agccaacaag agcatacact
     1441 aaatctgttc cacttgctat gtaaaactcc tttattccat tatagagctt atttactatc
     1501 tttttattag tatcatatct gtctgctccg cccaatcgag ttgaattagt atcattaact
     1561 agtttgtcac tcattgatga agtaccacct attgcataac tttctattcc agtagtatta
     1621 aatggtacac ttttcccatc agttaatatt ataggtgctt tgtctctaac agccacagag
     1681 gcaatactca tggcatctgg ttcacctttg aaagcattag ttaatattac cttatttact
     1741 ttattaatag aatttatttc ttttgctaca ttataacttg ttttaattct atcatttcct
     1801 tgaagtcttt tagtctctat tcctttatct tttagaaaag tttctgttgc cttatcaata
     1861 gaactttctc caccaattat gtacactttg ttagctttct ctactctttt aagagttgca
     1921 ttaggtatat tatttttctt agtcaaaaga attggtgcat ttgtagctcc tgcaagccca
     1981 cttgcactta acccatccgc cattgtacta tctgcattta taagtattgc agtagcataa
     2041 ctttgtttgt ctgcgataag tccagcagtt tcatatttat ctgctccttg aatcttatcg
     2101 attttatcaa gtgcatttgc agatatagga taacaagcaa caaacattga catagctaac
     2161 cctagcgata ataatttctt tgatttcaca atatctcccc ctttttcata tcactcaatt
     2221 aaattgtata acaaaaatat attatatgtt gtcgaaagta caaactattt ctacttaatt
     2281 aacgacatta aataacatta gaataaatta ttttcttata atctcattct ttataatata
     2341 tataattgtt ataatataat taaagcattt tactttaacc acattattta attttgagag
     2401 gagttattat tattaagggt ggagttagaa aaaaagggaa aaagtggtat tattattttg
     2461 atttaggagt tatagatgga aagagaaaaa aagtagaaag agctggggga aatactaaaa
     2521 aagatgctga aaaagcactt cgagaagcat tgaaagaata tgagaataca ggaataatgt
     2581 ttgatgagtg tgaaatgaac ttagcagaat atctagattt ttggtttaat aaatatgtta
     2641 ttttaaactg taaatataat actcaagaaa gttatagaat acacattcaa acacatatta
     2701 agccagcatt aggacattat aaattaaaat ccctaacacc agctacatta caaaacttta
     2761 ttaatgctaa gtttagaagt aactattctc aaagtacact tgaagttatt agggctattt
     2821 taaaaaagct ttaaaatcag ctgtctaccc atatcagttt ttgaaagata acccaatgca
     2881 atatatcgca attcctaaaa ataagactat aaaaaaagaa attaaagtaa tcacgttaga
     2941 tgaatttaac aatattttaa atttatttaa taaaggaact tatcaatata tatcattaat
     3001 ggttgctttt catactggaa tgagaaaagg tgaagttgct ggtctaactt gggaggatat
     3061 aaatctaaaa acaaaaacta tagatttata tcctcctatg ataaaaaaga aaaaaggaga
     3121 tattgaatta gcaacaccaa aatcctctag ttcttataga acaattacta taggtgatac
     3181 tttagtgaaa gctctaaaag aacataaaaa gtatcaaaat gaaaataaat tgctatttgg
     3241 tgaattttac ataaacaaca acttcgtatg tacaaaaata aatggaagtc caataaatat
     3301 caattctatt tctactatgt gcaatggtat tattaaaaaa ataaatactc attttacttt
     3361 tcatagttta agacatactc atgcaacaat gcttctagaa gctggggcaa atataaaagc
     3421 aatacaaaaa aggttaggtc atagtaatat cgcagtaact atgaatacat actctcatgt
     3481 gacagaaact atgaaacaag aaactgtaga tttatttgaa aaatccataa aataaaattc
     3541 ttccaccgaa aaataattac gatggaagaa tggtggaaga ctcctttaaa aatgttatat
     3601 ttatagtttc acttatattc gttcaactca ctttacctct tggtattact gtattcaaac
     3661 atatactaaa tataaatata atttatctaa ataaattttt ca
//
LOCUS       DAEPXF010000273         3596 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain HB27
            SAMN13376389-rid12479423.denovo.281, whole genome shotgun sequence.
ACCESSION   DAEPXF010000273 DAEPXF010000000
VERSION     DAEPXF010000273.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13376389
            Sequence Read Archive: SRR10518432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3596)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3596)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001069624.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001069624.1
            Genome Coverage       :: 315x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/15/2021 01:54:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,165
            CDSs (total)                      :: 4,076
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 89
            rRNAs                             :: 10, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 10 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 19 of 69
            Pseudo Genes (incomplete)         :: 44 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 10 of 69
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3596
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13376389-rid12479423.denovo.281"
                     /strain="HB27"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="China: Hebei"
                     /collection_date="2017"
     gene            complement(327..527)
                     /locus_tag="KRL67_003693"
     CDS             complement(327..527)
                     /locus_tag="KRL67_003693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003723180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HBH1524527.1"
                     /translation="MNNGIVKWFNNEKGFGFISMESGDDVFAHFSAIQTSGFKSLEEG
                     QKVSFDIVKGARGPQAENITIL"
     gene            complement(751..927)
                     /locus_tag="KRL67_003694"
     CDS             complement(751..927)
                     /locus_tag="KRL67_003694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH1524528.1"
                     /translation="MFRDKMDKCTHMLTAYIGSSYDYCDFIDTQLDDFIIEYGEKVVE
                     SCLHQVMVLVSKYN"
     regulatory      complement(981..1070)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            complement(1250..1450)
                     /locus_tag="KRL67_003695"
     CDS             complement(1250..1450)
                     /locus_tag="KRL67_003695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBH1524529.1"
                     /translation="MTKFICNLKKYRQLKELTQEQLAEKVGVRRETIMRLEAGKYNPS
                     LKLAIDISKIVDTPIDELFIFD"
     gene            complement(1452..1802)
                     /locus_tag="KRL67_003696"
     CDS             complement(1452..1802)
                     /locus_tag="KRL67_003696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3796 domain-containing protein"
                     /protein_id="HBH1524530.1"
                     /translation="MIQKKFFMGFCGFLGFLSLKYFSSGNIADLTYIGFFAFFSNFII
                     SKINGDKADERYVQDEKAAMAFTGQFAIIELFILWCIAIVSRNVELMCVLLSITYAVT
                     LNVYAIKLYILEEK"
     gene            1963..2151
                     /locus_tag="KRL67_003697"
     CDS             1963..2151
                     /locus_tag="KRL67_003697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH1524531.1"
                     /translation="MTKYDFNLKLKVAKFYLNREGGDVLIVKQYGVYGHSQVERWVNI
                     LGESALKIKKFSILYGLS"
     gene            2711..3088
                     /locus_tag="KRL67_003698"
     CDS             2711..3088
                     /locus_tag="KRL67_003698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HBH1524532.1"
                     /translation="MKEEILIEKLLRAELIVMEYLWKKDTLVPKKEIVKEIKQIYGWH
                     KSTIKILLKRLVDKRYLARYIIKFQSHYKIIIDNKEYYTFKKKVLKSSKSIKIMRSLT
                     TTHKNVSKEKLDLLDEYYRNLKE"
     gene            3193..3566
                     /locus_tag="KRL67_003699"
                     /pseudo
     CDS             3193..3566
                     /locus_tag="KRL67_003699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430377.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
BASE COUNT         1453 a          422 c          435 g         1286 t
ORIGIN      
        1 ttttaattgc attttattga caaattacta aaaaatgttc tatcagaatt gaatacaaaa
       61 gaaagagtat gtatttatgg gatagttgaa aattttgatg tgctacaact aaaattattc
      121 tattactata tattttaatt aaaagagtac ttgtgagttt aagtacatac tttataaata
      181 tatttttgtg agctttattt tataaaaagt atttagacta agtatttttt taagtattct
      241 cttgttgaag ttattttttt cttttttaca aaataccgta aataaaaaaa agactcttaa
      301 aagagtcttt ccgaaaaatc tatatattat aatatagtta tattttctgc ttgaggacct
      361 ctagcgcctt taactatatc aaagcttact ttttgacctt cttctaatga tttaaatcct
      421 gaagtttgta tagctgagaa atgagcaaat acatcatctc cactttccat tgatataaat
      481 ccaaatcctt tttcattatt aaaccatttt actattccat tattcattaa aaatactcct
      541 attaacctat atcaagatat acttgattta cttgtgtatt accacaaata ctattgtatc
      601 atagattaac ataaatgtct atttaattat aatttttagt taacattttt atttattctt
      661 aatatgaaaa taaatttaaa cattttatat taccaatata ttaatgtaaa tgtagataaa
      721 actaaaaagg gttttctaga aaaaatctgt ctaattatac ttacttacca ataccatcac
      781 ttgatgcaag caagattcca caactttttc tccgtactct attataaaat catctaactg
      841 tgtatctata aaatcacaat aatcatatga actaccaata taagcagtta acatatgtgt
      901 acatttatcc attttatctc taaacataat gtatttatcc ccctaaaaaa ctttcatgaa
      961 aaacaaaaag aaaatatctt tttggcaact ggctgacata acttatttta taagcgttag
     1021 tccctatagc tttgcgtcac taaatttctc tagttttgcc gatttagttt tattctacag
     1081 attaataata caatagaatt agttattatt caacatatct agctgaaaat ttaataaatt
     1141 tatatacaaa cgttaaatta tttgaaaaaa taaaaagccc ctctgttagg agtgactcaa
     1201 aaactgaatt taagtttgta tttaaaacac atttttaata atgcaaaaac taatcaaaaa
     1261 taaacaattc atcaataggt gtatctacta tttttgatat atctattgct aattttaaag
     1321 aaggattata ctttcctgcc tctaaacgca taatagtttc tcttctaact cctacttttt
     1381 ctgctaattg ttcttgagtt aattctttta attgtctgta tttttttagg ttacaaatga
     1441 atttggtcat attatttttc ctctaaaata taaagtttta tagcataaac atttagagta
     1501 actgcatatg taattgatag taaaacacac attaattcta cattcctaga cactatagca
     1561 atacaccata aaatgaataa ttctataata gcaaactgtc ctgtaaatgc catagctgct
     1621 ttttcatctt gtacatacct ttcatctgct ttatcaccat ttatttttga tataataaag
     1681 tttgagaaaa aagcaaaaaa tccaatatat gtaaggtcag caatattgcc agatgaaaaa
     1741 tatttaaggc ttaaaaatcc aaggaaacca caaaaaccca taaaaaattt tttctgaatc
     1801 attttttatc ctccttagta atttaaaaag agatatattt gtatctttat gatataaata
     1861 tatctctttt atattctttt gtcaatagaa aagcagtatt atacaatatt tgtactgcac
     1921 tataatgtta gagcaaaaat ctagcgcttg agatgtttat gtatgactaa atatgatttt
     1981 aatttgaaat taaaagtagc taaattttat ttgaatagag aaggaggaga tgttttaata
     2041 gtaaaacaat atggagttta tggccattct caagttgaaa gatgggttaa tatattagga
     2101 gagagtgcac taaaaataaa aaaattttct atactatatg gtttaagtta gatgttataa
     2161 actatatgat aataactaaa tatagtgtaa aataagtcgc taaatatttt ggaataaaca
     2221 atagcacagt tatagcaaca tgaaaacaaa aatttttaca tagtggtgtt gatgtctttt
     2281 caaaaaatta tataaactat cggtataaga aaacaaaatg tgtatacatg aatacatatt
     2341 tttttaaaga tatatattat tacacagtta aaaaactttc ataaaaacaa aaataaaata
     2401 tgcttttagt aacttgttag cataacttat aatttatttt ataagcatta gcctttatag
     2461 ctttgtgcca ctaaatttct ttgactctaa cgatttagtg ctattctaca actgaaataa
     2521 tacaatagag ctagtcattg tttggtagaa ttgtttgcaa attaaacaaa tttatatata
     2581 tgaatattat tttacactta aaaaaacaga ctaaatatgt agaatatttg gtaaaactta
     2641 cctctagttt ttgtcttaaa aagctcctat tattaattta agttagacga atttaaggaa
     2701 gtgacataaa ttgaaagaag aaatattaat agaaaaatta ttaagagcag aattaatagt
     2761 aatggagtat ttatggaaaa aggacactct agtgcctaaa aaagaaattg taaaagaaat
     2821 aaaacagatt tatggatggc ataaaagtac aataaaaata ctactaaaaa ggctagttga
     2881 taaaaggtat ttggctagat atattataaa atttcaatct cattataaaa taataataga
     2941 taataaagaa tattatactt ttaagaaaaa ggtactaaaa tctagtaaaa gcataaaaat
     3001 aatgcgttca cttaccacta cacataaaaa tgtaagtaaa gagaagttag acttattaga
     3061 tgagtattat agaaacttaa aagaatgata tagtaaaaat cgaatgtaat tattcacctt
     3121 aattcttttg tttgtaaaac tttattgata tagaatttaa catatacatg aattggtaaa
     3181 gggatgtata ctataaaaat tagtaaattg ccagaagcgg aattaaaagt tatgagatat
     3241 atttgggaat cagacaaggt attaatatca agagaaatca ccaagtctat ggaacaaaat
     3301 atgaatggaa agatacaact atatttacag tattaaaaag attacaaaga agagagtttt
     3361 tagaaacgga gaaaatagat aagcgtacac actataatgt attagtaaaa gaaaaataat
     3421 atcagagatt ttaaacaaaa gaatttttaa agaatacaca taaaaattca atattaagtt
     3481 taatgtcagc actatgtagt aaggatgatg aagtagatgg tgatatttta aatttattag
     3541 aagaatattt taagaatttg aaataagaaa atgacaaatt gatacataat aaataa
//
LOCUS       DAEPXF010000274         3595 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain HB27
            SAMN13376389-rid12479423.denovo.282, whole genome shotgun sequence.
ACCESSION   DAEPXF010000274 DAEPXF010000000
VERSION     DAEPXF010000274.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13376389
            Sequence Read Archive: SRR10518432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3595)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3595)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001069624.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001069624.1
            Genome Coverage       :: 315x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/15/2021 01:54:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,165
            CDSs (total)                      :: 4,076
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 89
            rRNAs                             :: 10, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 10 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 19 of 69
            Pseudo Genes (incomplete)         :: 44 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 10 of 69
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3595
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13376389-rid12479423.denovo.282"
                     /strain="HB27"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="China: Hebei"
                     /collection_date="2017"
     gene            complement(18..383)
                     /gene="rplL"
                     /locus_tag="KRL67_003700"
     CDS             complement(18..383)
                     /gene="rplL"
                     /locus_tag="KRL67_003700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000159736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HBH1524533.1"
                     /translation="MTIEQILEAIENMKVLELNELVKAAEEKFGVSASAPVMVAGAAA
                     GGPAAEEKTEFDVVLTDVGSSKVGVIKAVREITGLGLKEAKEVVDNAPKTVKEGASKE
                     EADQIKEKLEAAGAKVEVK"
     gene            complement(441..947)
                     /locus_tag="KRL67_003701"
     CDS             complement(441..947)
                     /locus_tag="KRL67_003701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HBH1524534.1"
                     /translation="MRKAIEIKSEVVSEIVEKLQKSSAAVVVDYKGLTVEEVTELRKQ
                     MREAGVDYKVYKNTLVRRAAKEVGIEQFNDELLVGTNAIAFGYDDPVAPARILKGFMD
                     SHPKMKLKMGIVEGAFYDESKIVEMANIPSREVLIAKLLGSLKAPVSNFAYLIDAIAK
                     KAEGQEEA"
     misc_feature    complement(990..1118)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00557"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L10 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00557"
     gene            complement(1170..1868)
                     /gene="rplA"
                     /locus_tag="KRL67_003702"
     CDS             complement(1170..1868)
                     /gene="rplA"
                     /locus_tag="KRL67_003702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HBH1524535.1"
                     /translation="MAKKGKRYAGALQKVDRTKFYDASEALTLVSDIAGAKFDETVEA
                     HIKLGVDSRHADQQVRGAVVLPHGTGKTKRVLVFAKGEKAKEAEQAGADFVGAEELVQ
                     KIQGENWFDFDIVVATPDMMGVVGRLGRVLGPKGLMPNPKSGTVTFDVAKAIDEIKAG
                     KVEYRLDKTNIIHVPVGKVSFGGEKLTENFTALMDAIIKAKPAAAKGQYLRSITVASS
                     MGPGVKINPARTAE"
     gene            complement(1939..2364)
                     /gene="rplK"
                     /locus_tag="KRL67_003703"
     CDS             complement(1939..2364)
                     /gene="rplK"
                     /locus_tag="KRL67_003703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012158357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HBH1524536.1"
                     /translation="MAKKVIGQIKLQIPAGKATPAPPVGPALGQHGVNIMGFTKEFNA
                     KTADQAGMIIPVVITVYQDRSFSFITKTPPAAVLIKKALNLKSGSGEPNKKKVAKMTS
                     AQVREIAELKMPDLNAASVEAAMSMIAGTARSMGVVIED"
     gene            complement(2403..2945)
                     /gene="nusG"
                     /locus_tag="KRL67_003704"
     CDS             complement(2403..2945)
                     /gene="nusG"
                     /locus_tag="KRL67_003704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination factor
                     NusG"
                     /protein_id="HBH1524537.1"
                     /translation="MSELQEASWYVVHTYSGHENKVKATIEKAVKTRGMEDCIRQVVV
                     PTEEVVETTKTGKEKTRQRKVYPSYVLVKMIITDESWYVVRNTKGVTGFVGPGSKPVP
                     LSEDEVKAMGIDTTDPKVVNSDIDFEIGDTVKVSQGPFSGQIGNIEEIDLENREVKVC
                     INAFGKRTLFVIELEGIEKI"
     gene            complement(2965..3186)
                     /gene="secE"
                     /locus_tag="KRL67_003705"
     CDS             complement(2965..3186)
                     /gene="secE"
                     /locus_tag="KRL67_003705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HBH1524538.1"
                     /translation="MAAQKNDGAKTKKRFSLFGYLKETKQELKRVTWPTKKELFKNTS
                     IVLTVVISCTILVWGIDTILSGALALLLK"
     gene            complement(3212..3361)
                     /gene="rpmG"
                     /locus_tag="KRL67_003706"
     CDS             complement(3212..3361)
                     /gene="rpmG"
                     /locus_tag="KRL67_003706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004402360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HBH1524539.1"
                     /translation="MRVKVTLACTECKQRNYNTTKNKKNNPDRIELQKYCRFCKKHTT
                     HKETK"
BASE COUNT          977 a          729 c          440 g         1449 t
ORIGIN      
        1 gtatcttttt aaagaaacta cttaacttct actttagctc cagcagcttc taatttttct
       61 tttatttgat cagcttcttc tttagaagct ccttctttaa ctgtcttagg agcattgtca
      121 actacttctt tagcttcttt taatcctaat cctgttattt ctctaactgc ttttataact
      181 ccaacttttg aagaaccaac gtcagttaat actacatcaa actcagtttt ttcttcagca
      241 gctggtccac cagcagctgc accagctacc attactggag ctgaagctga tacaccgaat
      301 ttttcttcag cagctttaac taattcattt aattctaaaa ctttcatatt ttctatagct
      361 tctaatattt gttctattgt catttttagc acctccgaat tttatttcaa attaatattt
      421 ataatttttt agttaagcaa ttaagcttct tcttgacctt ctgccttttt cgctattgca
      481 tctattaagt atgcgaagtt tgatactgga gcttttaagc taccaagtaa tttagcgata
      541 agaacttctc ttgatggtat gttagccatt tcaacaatct tagactcatc atagaatgca
      601 ccttccacaa tacccatctt taatttcatt tttggatgag aatccatgaa tccttttaat
      661 attctagctg gagcaactgg atcatcatat ccaaatgcta tggcattagt tccaactaat
      721 aattcatcat taaattgctc tataccaact tctttagctg ctcttctaac taaagtattt
      781 ttgtatactt tgtaatctac accagcttct ctcatttgtt ttcttaactc agttacttct
      841 tcaactgtta atcctttgta atctacaaca acagcagcag aagatttttg taacttttca
      901 actatctcag aaacaacttc tgatttaatt tctatagctt ttctcatata gatgtgcacc
      961 tccttcacca aataggctta gtatttaccg caacattata aaaagctttt gttgccaaaa
     1021 gtagacatca aaagctttag taatctcaat ttatctaaaa cctcggcggg atattaagct
     1081 ttcgcaccca ctatctacgg caaatctatt cttttttaca ttgacaagtg atataataac
     1141 atttttgtca acctgtgtca acagttttat tattcagctg ttcttgctgg gtttatttta
     1201 actccaggtc ccattgaaga agctactgtt atacttctta aatattgtcc tttagcagca
     1261 gctggtttag cttttattat agcatccatt aatgcagtaa agttttcagt taatttttct
     1321 ccaccaaatg atacttttcc tactggaacg tgtattatgt tagttttgtc taatctgtat
     1381 tcaactttac cagcttttat ttcatctata gctttagcta catcaaatgt aactgttcct
     1441 gatttagggt ttggcattaa acctttaggt cctaatactc tacctaatct acctactaca
     1501 cccatcatgt ctggagtagc aactactata tcaaagtcaa accagttttc accttgtatt
     1561 ttttgaacta attcttcagc tcctacaaag tcagcaccag cttgttcagc ttctttagct
     1621 ttttcacctt tagcaaaaac taaaactctt tttgttttac cagttccatg aggtaataca
     1681 actgcacctc ttacttgttg gtcagcatgt cttgagtcaa cacctaattt aatgtgtgct
     1741 tctactgtct catcaaattt agctccagct atatctgaaa ctaatgttaa cgcttctgat
     1801 gcatcataaa atttagttct atctactttt tgtaatgcac ctgcatatct tttacctttt
     1861 ttagccattt tattttcctc cttgtggtta atttcggctt ctcgcctccc actttataaa
     1921 aaaaagtgta tcttttaatt agtcttcaat tacaacaccc atgcttctag cagtaccagc
     1981 tatcatactc atagcagctt ctactgatgc agcatttaag tcaggcatct ttaattcagc
     2041 tatttctctt acttgagctg aagtcatttt agcaactttt ttcttgtttg gttctccaga
     2101 acctgatttt aagtttaatg cttttttaat taaaactgca gctggtggag tttttgttat
     2161 aaaactgaat gatctatctt gatatacagt tataacaact ggtattatca ttccagcttg
     2221 atctgcagtt ttagcattaa attcttttgt gaatcccatt atattaacac catgttgtcc
     2281 taatgctggt ccaactggtg gcgctggagt agcctttcct gcaggtattt gtaattttat
     2341 ttgacctata acttttttag ccattttgag cgcacctcct cagatttttc tataatatag
     2401 tattagattt tttctatacc ttcaagctct attacaaata gagtcctttt accaaatgca
     2461 tttatgcata ctttgacttc tctattttct aagtcaattt cttctatgtt acctatttgt
     2521 ccactaaatg gtccttgaga tacttttact gtatctccta tttcaaaatc aatatcgcta
     2581 tttactactt tagggtctgt tgtgtctatc cccatagctt tgacttcgtc ttcacttaat
     2641 ggcactggtt ttgaaccagg tccaacgaaa cctgtaactc ccttagtatt tcttactaca
     2701 taccaagatt catcagttat tatcatttta accaaaacat aacttggata aactttacgt
     2761 tgtctagttt tttcttttcc agttttagta gtttccacta cttcttctgt tggaacaact
     2821 acttgtctta tgcagtcttc catacctctt gttttaactg ctttctcaat tgtagcttta
     2881 actttatttt catgtcccga ataggtatga actacatacc aacttgcttc ttgtaattct
     2941 gacatttaac ttacctcttt tcgtctattt tagtaataac gctagtgcac cagataatat
     3001 agtgtctata ccccatacta atatagtgca tgaaataact acagttaaaa ctatacttgt
     3061 gtttttaaac agttcctttt ttgtcggcca tgtaactctt tttaattctt gtttagtttc
     3121 ttttaagtat ccaaacaaac taaacctttt tttagttttt gcaccgtcat ttttttgggc
     3181 agccattcca tactcacatc cttataaaaa actattttgt ttctttatga gttgtatgct
     3241 ttttacagaa tctacaatat ttttgtaatt ctattctgtc tggattattt ttcttattct
     3301 tagtagtgtt gtagtttctt tgcttacact ctgtacatgc taaagttact ttaactctca
     3361 tctgatacac ctccaacttt gtaatacaat aatatatatt gttaatatat gtgaatataa
     3421 tatctttaaa atatgtattt attaaaaatc tacagtattc aagatattat taacttccta
     3481 taatcattac cttgtataag ttatcacaaa atcctatgct gtgtcaatgt ttttgtattt
     3541 ctatgttaaa aaaagaggag cataacccct ctttttgtta ttccttttaa tccat
//
LOCUS       DAEPXF010000275         3540 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain HB27
            SAMN13376389-rid12479423.denovo.283, whole genome shotgun sequence.
ACCESSION   DAEPXF010000275 DAEPXF010000000
VERSION     DAEPXF010000275.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13376389
            Sequence Read Archive: SRR10518432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3540)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3540)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001069624.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001069624.1
            Genome Coverage       :: 315x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/15/2021 01:54:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,165
            CDSs (total)                      :: 4,076
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 89
            rRNAs                             :: 10, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 10 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 19 of 69
            Pseudo Genes (incomplete)         :: 44 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 10 of 69
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3540
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13376389-rid12479423.denovo.283"
                     /strain="HB27"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="China: Hebei"
                     /collection_date="2017"
     gene            92..208
                     /gene="rrf"
                     /locus_tag="KRL67_003707"
     rRNA            92..208
                     /gene="rrf"
                     /locus_tag="KRL67_003707"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            336..845
                     /locus_tag="KRL67_003708"
     CDS             336..845
                     /locus_tag="KRL67_003708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription repressor NadR"
                     /protein_id="HBH1524540.1"
                     /translation="MNSNERRSKLIDILKESKHPVKGGILAELLNVSRQVIVQDIALI
                     RARGFEIIATPQGYIIYNQPYFTKQIKCKNHKDSKEIYDELKIIVDLGGIIKDVIVNH
                     PTYGQITAELNIYSKMDIDNFMKKVETNEFKQLSVLTECSHIHTIEAIKEETIEAIVK
                     ELKDNGILS"
     gene            857..1738
                     /locus_tag="KRL67_003709"
     CDS             857..1738
                     /locus_tag="KRL67_003709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel"
                     /protein_id="HBH1524541.1"
                     /translation="MNFLSLAKTASTNSNITKRTLENMLDNFSDNIPNIIYAIIVFVI
                     GIYISKIVRRMVSKFLIKYGMSKGVNNFIVYGIYISMLSIISLISLGIIGIQTTSVVA
                     VLGAAGFSIGLAFKEILSNLGSGMIILFFKPFNIGDYIQGSGVEGTVSDIQIFSTVLK
                     TPDNKTIIIPNFQLTSNNIINYTHQNKRRIDFSYNISYDSDIDVVKSILNEIFTNEKR
                     ILNDPKPIIGLNSIGNNTMQIVARPWVKTDDYWDVYFDVMEKVKNKFDENDIKVPFVP
                     SSLLFNNTGNLNTMDKR"
     misc_binding    1866..2071
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            2197..3468
                     /gene="serS"
                     /locus_tag="KRL67_003710"
     CDS             2197..3468
                     /gene="serS"
                     /locus_tag="KRL67_003710"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000884181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HBH1524542.1"
                     /translation="MLDIKRIRENLDDIKKAMERRGEREFDLDAVVELDNKRREILQE
                     VEVMKNELNVNSKKIPQLIKEGKDVTEEKARLKELSDKIKGIDEKVKEVEAKMEYTLM
                     RIPNVPHPEVPQGETDEDNVQIRTWGEPTKFDFEHKAHWDIGTGLGILDFERAGKITG
                     SRFTLYRGLGARLERSLMNFFLNTHTAKHGYTEVLPPFMANRNSFIGTGQLPKFEEDM
                     FKIEGLEYFLIPTAEVPVTNIHANEILDVAELPIKYCAYTPCFRSEAGSAGRDTRGLV
                     RQHQFNKVELVKFVKPEDSYNELESLTHDAETMLQMLGLPYRVVKICTGDLGFTAAFK
                     YDLEVWMPSYNRYVEISSCSNFEDFQARRAGIRFKRDKKSKAEYVHTLNGSGLAIGRC
                     LAAILENYQQADGSVVVPEELRPYMGVDVIR"
BASE COUNT         1428 a          351 c          630 g         1131 t
ORIGIN      
        1 agtttttaga gtgttaactc taaagaaact agtattacta ggttctataa acttattaaa
       61 aacttaatag aaatattaag cataaagatt atgtggttac aatagcagag aggatacacc
      121 tgttcccatt ccgaacacag aagttaagct ctctagcgct gatggtactt ggtgggaaac
      181 tgcctgggag agtaggacgt agccacgtag tcttttttta tttattataa aagttaagat
      241 taaatagaat ataaagtcaa tattgattta taataagata aaatataaaa ttatatagta
      301 taggtaaagt tttttattta gaaagaaggt gtaatatgaa ttctaatgaa agaagaagca
      361 aattaataga tattttaaaa gaatctaagc atcctgtgaa aggtggaata ctagctgaac
      421 tattaaatgt aagtaggcaa gtaatagttc aagatatagc ccttataaga gctagaggat
      481 ttgaaataat agctacacct caaggatata taatatataa tcaaccgtat tttacaaaac
      541 aaataaaatg taaaaatcat aaggattcta aagaaattta tgatgaatta aagataattg
      601 tagatttagg tggtattata aaagatgtca ttgtaaatca tccaacatat ggtcagataa
      661 ctgcagaact taatatttat tctaaaatgg atatagacaa ctttatgaag aaagttgaga
      721 caaatgaatt taaacaatta tcggttctta cagaatgtag ccatattcat acaattgagg
      781 caataaaaga agaaactata gaagctatag taaaagagtt aaaggacaac ggaatattat
      841 cataaggagg agttaaatga attttttatc attggctaag actgcctcaa caaatagcaa
      901 tataactaag agaactttag aaaatatgct cgataatttt agtgataata taccaaatat
      961 tatatatgca ataattgtat ttgtgatagg tatttatata tctaaaatag taagaaggat
     1021 ggtttcaaag tttctaatca aatatggaat gagtaaaggt gtaaataatt ttattgtata
     1081 tggaatatat atatctatgt tatctattat atcattgata agtttaggca ttatagggat
     1141 acaaactaca tcagttgtgg ctgtgttagg agctgctgga tttagtatag gtttagcttt
     1201 taaagaaata ttatcaaatt taggttctgg tatgattatt ttatttttta agccatttaa
     1261 tataggtgat tatattcaag gttcaggtgt agaaggtaca gtttctgaca tacaaatatt
     1321 tagcactgtt cttaagactc cagataataa aactataatt atacctaatt ttcaactgac
     1381 aagtaataat ataattaatt acacacatca gaacaaaaga agaatagatt tttcatataa
     1441 tatttcatat gatagtgata tagatgtagt taagtctata ttaaatgaaa tttttacaaa
     1501 cgaaaaaaga atattaaatg atccaaagcc tataataggt ctaaactcta taggaaataa
     1561 caccatgcaa atagtcgcta gaccttgggt aaaaactgat gattactggg atgtatactt
     1621 tgatgtaatg gaaaaggtaa aaaataaatt tgatgagaat gatataaaag ttccatttgt
     1681 tccttcttca ttattattta ataatacagg taatttaaat acaatggata aaagataagg
     1741 tcttaattaa atagaaaaaa tgaatttaat ataaaaaatt aaagaaaatt ctaaaaaaat
     1801 ttgaataata gtattgaaag atgttaatat atataatata atgattatca aataaaacaa
     1861 ttagttaaaa agctgtgaag gaaaatagta aaaaacagat tgtattcaga gaatgagtgg
     1921 atggtgaaaa ctcattacaa tggtttttga atctactcta gagcttctag ttaaagctag
     1981 acgtttacct acgttatagg tctgagagga ttatacattt tagtatagtc aattagggtg
     2041 gcaacgcgga taaaagattt tcgtcccttt tatagggtaa acggaggtct ttttttattt
     2101 ttaaaatttg tttaaactaa gagtttacaa gtataatgtt tgtaaattaa gttggaatta
     2161 taacttatat tatcaaaatt attaggagga attaatatgt tagatattaa aagaataaga
     2221 gaaaatctag atgatatcaa aaaagctatg gaaagaagag gtgaaagaga atttgacctt
     2281 gatgctgtag tggaattgga taataaaaga agagaaatac ttcaagaagt tgaagttatg
     2341 aaaaatgagt taaatgtaaa ttctaaaaaa atacctcaat taataaaaga aggaaaagat
     2401 gtaacagaag aaaaggcaag attgaaagag ttatcagata aaataaaagg tattgatgag
     2461 aaagtaaaag aagtagaagc aaaaatggaa tatacattaa tgagaatacc aaatgttcct
     2521 catccagagg taccacaagg tgaaacagat gaagataatg ttcaaataag aacttgggga
     2581 gagcctacta aatttgattt tgaacataaa gcgcattggg atataggtac aggtctaggt
     2641 attttagatt ttgaaagagc agggaaaata actgggtcaa ggtttacttt atatagggga
     2701 ttaggagcta gattagaaag atctttaatg aatttctttt taaatactca tacagctaag
     2761 catggctata cggaagtctt acctccattt atggccaata gaaatagttt tataggtact
     2821 ggtcaacttc ctaaatttga agaagatatg tttaaaatag aaggattaga gtatttctta
     2881 ataccaacag ctgaagttcc tgttactaat atacatgcta atgaaatatt agatgttgca
     2941 gagcttccaa ttaaatactg tgcatatact ccttgtttta gatctgaagc aggttcagca
     3001 ggaagagata caagaggttt agttagacaa catcaattta ataaagttga attagttaaa
     3061 tttgttaagc cagaagattc ttataatgaa ttagaatcat taactcatga tgctgaaact
     3121 atgttgcaaa tgttaggtct tccatataga gttgtaaaaa tatgtacagg agatttggga
     3181 tttacagctg catttaaata cgatttagaa gtttggatgc caagttacaa tagatatgta
     3241 gaaatatctt cttgttcaaa ctttgaagat ttccaagcaa gaagagcagg aataagattt
     3301 aaaagagata aaaaatcaaa agcagaatat gttcatacat taaatggttc tggattagcg
     3361 ataggaagat gtttggctgc tatattagaa aattatcagc aagcagatgg ttctgtagta
     3421 gttccagaag aattaagacc ttatatgggt gttgatgtta taagataatt ttatatttat
     3481 tgtatatgta gagtatgctt taatgaagag aatatattat gagtaaacaa taaattaaaa
//
LOCUS       DAEPXF010000276         3521 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain HB27
            SAMN13376389-rid12479423.denovo.284, whole genome shotgun sequence.
ACCESSION   DAEPXF010000276 DAEPXF010000000
VERSION     DAEPXF010000276.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13376389
            Sequence Read Archive: SRR10518432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3521)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3521)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001069624.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001069624.1
            Genome Coverage       :: 315x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/15/2021 01:54:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,165
            CDSs (total)                      :: 4,076
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 89
            rRNAs                             :: 10, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 10 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 19 of 69
            Pseudo Genes (incomplete)         :: 44 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 10 of 69
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3521
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13376389-rid12479423.denovo.284"
                     /strain="HB27"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="China: Hebei"
                     /collection_date="2017"
     gene            6..659
                     /locus_tag="KRL67_003711"
     CDS             6..659
                     /locus_tag="KRL67_003711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HBH1524543.1"
                     /translation="MTKIIKVQKPKFTGELEIIENLEDILEDIFNDEKIFNKIIENEV
                     ISDLDSVRVSFESCIFRNVSFENCNLKKIDLLDVVFENCNLSNTFFDGGSIYRVEFKN
                     SKLIGVRFDDCILKNVLFKDVLGKYSNFSFSKFKIVSILDSNFENSVFQEVKGDNLVF
                     EYTNLKKANFNGTKLSGIDFTTNNIEGIEIGIDDIRGAIFDVSQAIDLTKLMGIIIK"
     gene            800..2071
                     /gene="brnQ"
                     /locus_tag="KRL67_003712"
     CDS             800..2071
                     /gene="brnQ"
                     /locus_tag="KRL67_003712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004455013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HBH1524544.1"
                     /translation="MRDIFIVGFALFSMFFGAGNLMFPPFLGMESGKDWLIPLIGFVV
                     ADAGIAMLVIIATARCKGSMDDVLRRAGKGLARVMSVAALACLALLVIPRTCATTYEM
                     GIMPIFGDHGPVAKAIFSIIFFGLTLAFTIRSSKVIDIIGKYLTPALLLVLFVLIVKG
                     IISPAGPMSPEHMIDKNLFGEGISQGYQTMDALGAASMATIMLMSIIAKGYTSDKDQI
                     GMTIKAGFVACVFLAVVYGGLTYLGATVSTLYDTSIPQASLLVEITSILLYGQVGKVI
                     LGVIVALACLTTSTGLTASISTYFEGISKASYKQLVIGICVASMLISNLGVDSIIAIS
                     VPILQTIYPVLLAIVVMELAGKHIKNDNAFKGAAYVTLVISLLSAINGMTGAVPFIQS
                     LPLAGLGFNWVIPAILGGIVGNFIKSNKQLA"
     gene            2273..3460
                     /locus_tag="KRL67_003713"
     CDS             2273..3460
                     /locus_tag="KRL67_003713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3798 domain-containing protein"
                     /protein_id="HBH1524545.1"
                     /translation="MLRKITAFMLSICLIIGLTGCSLLVGKKDKEKIIDDFKVAVVTQ
                     PLSENKVQYNMVEEMAKEYEEENKIDKDKDGQTKVKQTIKHVVLPENFTSNIDSAINK
                     IVKLADDEEVQAIVVSTDQAGLLPALQKVKEKRPEIITISAPMGDDKNQLSQFVDVNL
                     GVSAEERGKVLAERSKEMGAKAFIHYASTDDLKDANIAKRLEMIKETCKNIGLPFVQV
                     NTPNINTEEDKNKVKQFLNEDIEKQVKKYGKDINVFGVNEYMDEVILTKALELKYIVA
                     EQSNPSPIQTYPSVMGLKISEKDAQNYDKINDMISEKAKAFGMSNRLGGYPMPMDAFL
                     PSLAIYLATEMVKQDLTQEDVCDPDYLEAFTELRFGIGSEFTPLTEVLYNYQSVILSQ
                     LIY"
BASE COUNT         1284 a          393 c          658 g         1186 t
ORIGIN      
        1 ggaaaatgac taaaattatt aaagtgcaaa aacctaaatt tacaggagaa ttagaaatta
       61 ttgaaaatct tgaagatata cttgaagata tatttaatga tgaaaaaata tttaataaaa
      121 ttatagaaaa tgaagttata agtgatttag attctgtaag agttagcttt gaatcttgta
      181 tatttagaaa tgtatccttt gaaaattgca atttaaaaaa gattgattta cttgatgtgg
      241 tatttgaaaa ctgcaattta tcaaatacat tttttgatgg aggaagtata tacagagttg
      301 aatttaaaaa tagtaaattg ataggagtta gatttgatga ttgtatactt aaaaacgttt
      361 tgttcaagga tgttttaggt aagtattcta acttttcgtt ctctaaattt aagatagttt
      421 ctatcttaga ttcaaatttt gaaaattctg tatttcaaga agtaaaaggt gacaatctag
      481 tatttgagta tacaaactta aaaaaagcaa actttaatgg aactaaactt tctggcatag
      541 attttactac taataatatt gagggaattg agattggtat agatgatatt agaggagcga
      601 tttttgatgt gtcgcaagca attgatttaa cgaaattgat gggaattatc ataaaatgaa
      661 aaaaatattc atgaaataaa ttgggaaaac gttaacaatg tgtgaatttt taccagattg
      721 acatttttct gaaaataaag taagattatc ttaatattca aaatattttc aaagggggta
      781 atttagtgtg agtaagcaga tgagagatat ttttatagtt ggttttgctt tattttctat
      841 gttctttggt gcaggaaatc ttatgtttcc tccattttta gggatggaat ccggcaaaga
      901 ctggttaatt cctcttattg gatttgttgt tgcagatgct ggtatagcaa tgcttgttat
      961 tatagcaaca gcaagatgca agggaagcat ggatgatgta cttaggagag ctggtaaagg
     1021 gttagcaaga gtaatgagtg tagctgctct agcatgttta gcacttcttg taataccaag
     1081 aacttgcgca acaacatatg aaatgggtat tatgcctata tttggagacc atggcccagt
     1141 agctaaagct atattttcaa taatcttctt tgggttaact ttagcattta ctattaggtc
     1201 ttctaaggtt atagacataa taggtaagta tttaactcca gcgttattat tagtattatt
     1261 tgttttaata gtaaagggta taatttctcc agcaggacct atgagtcctg agcatatgat
     1321 agataaaaat ctatttggtg aaggtatatc acaaggttat caaacaatgg acgctctagg
     1381 tgcagcatct atggcaacta taatgttgat gtctataata gctaaaggat atacatcaga
     1441 taaagaccaa ataggaatga ctattaaagc tggttttgta gcttgtgtat ttctagcagt
     1501 agtatatggt ggtcttactt atctaggtgc aacagtttca actttatatg atactagtat
     1561 acctcaagca tctttattag ttgaaataac tagtatattg ttatatggtc aagtaggaaa
     1621 ggtaatttta ggtgtaattg tagcacttgc ctgtctgact acttctacag gtttaacagc
     1681 atcaatatca acttattttg aaggaataag taaggcgagt tataaacaac tggttatagg
     1741 tatatgtgta gctagtatgc ttatatcaaa cttaggtgtt gattctataa tagctatatc
     1801 agttccaatc ctacaaacta tatatcctgt tttattagca atagttgtta tggagttggc
     1861 tggtaaacat ataaagaatg ataatgcttt taaaggtgca gcatatgtta cattagtgat
     1921 tagtttatta tcagctataa atggaatgac aggagctgtt ccatttatac aaagcttgcc
     1981 acttgcaggt ttgggcttta actgggtaat accagctata ttgggtggaa tagttggtaa
     2041 ctttataaaa agcaacaaac aattagcata ataatttgta attaatttaa ttttataata
     2101 tattgatttt tagtactatg ggtaggttta atctaaacct aatctaccca tagtactttt
     2161 tagattggat tagaatagca tgtatataat tttttataat ataatttttc tttgaagtat
     2221 gataaaatta gtaatagtga taagaaagta caaatttagg aggttttaat taatgcttag
     2281 aaaaataaca gcatttatgc tatccatatg tttaattatt ggacttacag gttgtagttt
     2341 acttgtaggc aagaaagata aagagaaaat tatagatgat tttaaagttg cagtggtaac
     2401 tcaaccatta tctgaaaata aagtacaata caatatggtg gaagagatgg caaaagaata
     2461 tgaggaagag aataaaattg ataaagacaa agatggtcaa actaaagtta aacaaactat
     2521 aaagcatgtt gtattgccag agaattttac ttctaatata gatagtgcta ttaataagat
     2581 agtaaagttg gctgatgatg aagaagttca ggctattgta gtttcaactg accaagcagg
     2641 ccttttacct gccttacaaa aggtaaagga aaagcgtcca gaaataataa ctatttctgc
     2701 tcctatggga gatgataaaa accaattaag ccaatttgtt gatgtgaatc taggggttag
     2761 tgcagaggaa agaggaaaag tattagctga acgttcaaaa gaaatgggag caaaagcttt
     2821 tattcattat gcctctacag atgatttgaa agatgctaat atagccaaaa gattagaaat
     2881 gattaaagag acttgcaaaa atataggatt gccatttgta caagttaata ctccaaatat
     2941 aaatactgaa gaagacaaaa acaaagttaa gcaattttta aatgaagata ttgaaaaaca
     3001 agttaaaaaa tatggaaaag atataaatgt atttggtgta aatgagtata tggatgaagt
     3061 tatattgact aaggctttag aattaaagta tatagttgca gagcaaagca atccatcacc
     3121 aatacaaacg tatcctagtg ttatgggttt aaaaatatct gaaaaagatg ctcaaaatta
     3181 tgataaaata aatgacatga taagtgaaaa ggctaaggct tttggtatgt caaatagact
     3241 tggcggatat cctatgccaa tggatgcttt tctaccatca ttagcaattt atcttgctac
     3301 tgaaatggta aaacaagatt taacacaaga agatgtatgt gacccagact atttagaggc
     3361 gtttacagaa ttaagatttg gaatcggttc agaatttact cctttaacag aggtacttta
     3421 taattatcaa tcagtaattt taagccaatt aatttattaa tattagggaa gtgcctcaaa
     3481 atgaaatttt tagtttatga ggcactcttt ttgtgataaa t
//
LOCUS       DAEPXF010000277         3506 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain HB27
            SAMN13376389-rid12479423.denovo.285, whole genome shotgun sequence.
ACCESSION   DAEPXF010000277 DAEPXF010000000
VERSION     DAEPXF010000277.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13376389
            Sequence Read Archive: SRR10518432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3506)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3506)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001069624.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001069624.1
            Genome Coverage       :: 315x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/15/2021 01:54:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,165
            CDSs (total)                      :: 4,076
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 89
            rRNAs                             :: 10, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 10 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 19 of 69
            Pseudo Genes (incomplete)         :: 44 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 10 of 69
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3506
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13376389-rid12479423.denovo.285"
                     /strain="HB27"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="China: Hebei"
                     /collection_date="2017"
     gene            complement(63..1010)
                     /locus_tag="KRL67_003714"
     CDS             complement(63..1010)
                     /locus_tag="KRL67_003714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HBH1524546.1"
                     /translation="MFYFLKVKVGDIMDEVKEFLVLEEEEEVRLDIYLAEQLGDMSRS
                     YIQKIIKDGKVKVNNKVEKAKYLVKEEDNIVIEIPEPKVLEVLPQDIPIDIVYEDDDV
                     LIINKPQDMVVHPAPGNYENTLVNGILYHCKDKLSSINGVIRPGIVHRIDKDTSGLLM
                     IAKNNYAHNFLAEQLKEHTITREYEFICYGVVKEDKVTVDKPIGRNPKDRLKMAVVKE
                     GKNAITHFEVVERFDKFTHMRARLETGRTHQIRVHALSINHPLLGDYVYGPKETKFKL
                     KGQTLHAKKLGFIHPTTKEYIEFNSELPEYFKEVIKKIK"
     gene            complement(979..1425)
                     /gene="lspA"
                     /locus_tag="KRL67_003715"
     CDS             complement(979..1425)
                     /gene="lspA"
                     /locus_tag="KRL67_003715"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HBH1524547.1"
                     /translation="MLYILIIILLIGLDQLSKIWVLNNLVDVSTIPIINNVFHLTYVE
                     NRGAAFGLLQNNQWIFITVALLATVFGLYYLNTRKVHIFGRLGIILIISGALGNLVDR
                     VRLGFVVDYFDFRIIWEYVFNIADVFVVVGTVFLCIYVLFFESKSR"
     gene            complement(1735..2514)
                     /locus_tag="KRL67_003716"
     CDS             complement(1735..2514)
                     /locus_tag="KRL67_003716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HBH1524548.1"
                     /translation="MKKTIWGICICLLSIVGVYTYNHNTHNQDSSKEAYSLVDNIKDD
                     YEDVIIKKGNEDEKILALTFDDGPDEDFTPQVLDILKKNDVKATFFVVGEKVEYNKGL
                     LKRQYDEGHEIGNHTFTHINVAKNSYGSVEKEITDTQNIIKSVIGVEPKIFRPPYRAM
                     NKSVCDIIVSKGMNIILWSNLDPRDWSNPGVDTIINTILTKVQNGNIILLHDYNTKRN
                     DKSQTIQALEVVIPKLKERGYKFVTVSELIEHLDKNKQVQK"
     gene            complement(2569..3051)
                     /locus_tag="KRL67_003717"
     CDS             complement(2569..3051)
                     /locus_tag="KRL67_003717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA C4-type zinc finger protein"
                     /protein_id="HBH1524549.1"
                     /translation="MDTKKYKDKLLKERENLTNLVEDMKDNTLFGDTTKHTSEKYSSG
                     ELSSYDNHIGDMGTDLYMQNMQNSLINHEEGRLYQIDLALSKIENGTYGICDLCHNQI
                     DLERLDILPDTSLCNDCAKKENNLLGDAMQNPDVEDSNFYSEDLTNLTDLNKNGILRD
                     "
     gene            3336..>3506
                     /locus_tag="KRL67_003718"
     CDS             3336..>3506
                     /locus_tag="KRL67_003718"
                     /inference="COORDINATES: protein motif:HMM:NF013677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBH1524550.1"
                     /translation="MSKKHKKYSKELKLKAVNLYIKEGYSSYKIAEMLNIRSKTQVQN
                     WVKDYKNKGKTAF"
BASE COUNT         1189 a          581 c          337 g         1399 t
ORIGIN      
        1 tttcactaaa tttatttact tatataaaaa caaaaaatta tttataggta ttgtacaaat
       61 ggttatttta tcttcttaat aacttcctta aaatactcag gtaattctga attaaattcg
      121 atatattcct tagtagtagg atgtataaat cccaacttct tagcgtgcaa agtttgtcct
      181 tttaatttaa atttagtttc ttttggacca tatacataat cacctaaaag tgggtgattt
      241 atagacaaag catgaactct tatttgatga gttctaccag tttcaagcct agctctcata
      301 tgtgtaaact tatcaaatct ttctacaact tcaaagtgag tgattgcatt ttttccctct
      361 tttactacag ccatttttaa tctatcctta ggatttctcc ctataggttt atcaacagtt
      421 actttatctt ctttaaccac accatagcat ataaattcat actccctagt tatagtatgt
      481 tcctttaact gttctgctag aaaattatgc gcataattgt tcttagctat cataagaaga
      541 ccagaagtat ctttgtctat tctatgaact ataccaggtc taataactcc atttattgat
      601 gacaacttat ctttacaatg atataaaatc ccatttacca gcgtattttc atagtttcca
      661 ggagcagggt gaacaaccat atcctggggt ttatttatta ttaagacatc atcatcttca
      721 tatacaatat ctattggaat atcttgaggt aaaacttcta atactttagg ttctggtatc
      781 tctataacaa tattatcttc ctctttaacc agatatttag ctttttctac tttattatta
      841 acctttactt ttccatcttt tataattttt tgtatataac ttcttgacat atcacctaat
      901 tgttctgcta gataaatatc aagtctaact tcttcttcct cttcaagtac taaaaactct
      961 tttacttcat ccattatatc acctactttt actttcaaaa aataaaacat atatacataa
     1021 aaacacagtt cctacaacta caaatacatc agctatattg aatacatatt cccatataat
     1081 tctaaagtcg aagtaatcta ctacaaaacc taatcgtact ctatcaacta gatttcccaa
     1141 tgcaccagat ataattaata taattcccaa ccttccaaat atatgtactt ttcttgtatt
     1201 aagatagtat aatccaaata ctgttgcaag taatgcaaca gttataaata tccattgatt
     1261 attttgcaat aatccaaatg ctgcacctct attttcgaca taagttaaat gaaatacatt
     1321 atttattatt ggtattgttg aaacatctac caaattattc aatacccata ttttagacag
     1381 ttggtctaag cctatgagta gaattattat taatatatat agcaatgatt tttcccccta
     1441 taaattattt aaaaaccaat tttatttgaa acacactatt attattatac aaaaaaaatg
     1501 ctctaaagag caactttatt aaaattgtat tttcttaata aagctagaac aaaatttaaa
     1561 atagtatcac tttttttata ttgtagattt ctacatattt aaaatcatat tattatgtat
     1621 aaaaaattta aaaataccat actaatcaat tctttttgtg tattttatag attaaagatt
     1681 aatgatgtct gtactttaaa cttacaagca ccactaatct tcaaacaaat atttttactt
     1741 ttgcacttgc ttatttttat ctaaatgttc tataagttca gatactgtta cgaatttata
     1801 tcctctctct tttaatttag gaattactac ttctaaagct tgtatagttt gtgatttatc
     1861 atttctttta gtgttataat catgtaaaag tattatatta ccattttgga cctttgtaag
     1921 gatagtattt ataattgtat ctactccagg attagaccaa tctctagggt ctaagtttga
     1981 ccaaagaatt atattcatgc ctttagaaac tataatatca cagacacttt tattcatggc
     2041 tctatatgga ggtctaaata tctttggttc tactcctatt acacttttta taatattttg
     2101 tgtatctgtt atttcttttt caacactacc ataactattt tttgcaacat ttatatgtgt
     2161 aaatgtatga ttccctattt catgcccctc atcatattgt ctttttaata atcctttgtt
     2221 atattctact ttttctccaa ctacaaaaaa agttgctttt acatcatttt tctttaatat
     2281 atcaagtact tgtggtgtaa aatcctcatc tggaccatca tcaaaagtca atgctaaaat
     2341 tttttcatcc tcatttcctt tttttattat tacatcttca taatcatctt ttatattatc
     2401 aactaaacta tatgcttctt ttgaactatc ttgattatgt gtattatgat tatatgtata
     2461 tacacctact atagataata aacatataca tataccccat attgttttct tcatgtcatt
     2521 acctcttttc taattcattg tagcaatata ttatataatg tcagtttttt aatctctcaa
     2581 tatcccattc ttatttaaat cagttaaatt tgttaggtct tctgaataaa agttagaatc
     2641 ttctacatca gggttttgca ttgcatcacc taataaatta ttttctttct ttgcacaatc
     2701 attacataaa cttgtatctg gcaatatatc caatctttcc aaatctattt gattatgaca
     2761 taaatcacaa atcccatatg ttccattctc tattttagat aatgctaaat ctatttgata
     2821 caatcttccc tcttcatgat ttattaaact gttttgcata ttttgcatat ataaatctgt
     2881 tcccatgtct cctatatgat tatcataact agataattct cctgatgaat acttttcact
     2941 tgtatgttta gttgtatctc caaataaagt attatctttc atatcctcta ctaaattagt
     3001 taaattttct ctttctttca ataatttatc tttatatttt ttagtatcca tttttaacac
     3061 ctctaatttt aatatttata tcataaaaat agtatgtgga aaagtaaatt aaacatacaa
     3121 taaaaataag atttgattca aaaaatgaaa tgtctcaaaa tgaaattttt agttcattta
     3181 acctcctttt ttgtatgaaa tcaaacatat ttttatcctt tcaacaatga aaaagagact
     3241 tatctctatt ttagtataat ccccttatag tagacattta aataaaaggc tattataata
     3301 ggtctaaaaa gcgtatactc taagggggtt tttctatgtc taaaaaacac aaaaaatata
     3361 gcaaagaact taaactaaaa gctgtaaact tgtatataaa agaaggatac tcatcataca
     3421 aaattgctga gatgctgaat ataagaagca aaacacaagt tcaaaactgg gtaaaagatt
     3481 ataaaaataa aggtaaaact gcattt
//
LOCUS       DAEPXF010000278         3483 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain HB27
            SAMN13376389-rid12479423.denovo.286, whole genome shotgun sequence.
ACCESSION   DAEPXF010000278 DAEPXF010000000
VERSION     DAEPXF010000278.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13376389
            Sequence Read Archive: SRR10518432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3483)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3483)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001069624.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001069624.1
            Genome Coverage       :: 315x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/15/2021 01:54:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,165
            CDSs (total)                      :: 4,076
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 89
            rRNAs                             :: 10, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 10 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 19 of 69
            Pseudo Genes (incomplete)         :: 44 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 10 of 69
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3483
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13376389-rid12479423.denovo.286"
                     /strain="HB27"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="China: Hebei"
                     /collection_date="2017"
     gene            complement(70..915)
                     /locus_tag="KRL67_003719"
     CDS             complement(70..915)
                     /locus_tag="KRL67_003719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HBH1524551.1"
                     /translation="MFKEIFLRTFPGFLIIRDSNYRIIFINDNLKNLIKSYMQDNPLG
                     MTNIEIAKKLPDNIAKFFTDSHNIQLDWEKNYPYDKISNWILEFKKDTTSYWNVLEYK
                     VDVDEKPYIITMANDITKLYEENKRNLHYSITDPLTGAYNRKYLNDRFDIFIGDYIVL
                     IDLDNFKMINDYEGHNVGDKILCDFVSLLNKELINSTSIIRLGGDEFIVIFSSDVDKN
                     YVYSQLETLRENFLKVFSKYKYLSFSYGVDTVKRNLKLTIVELDKKMYKNKEKNKKKF
                     DKNDY"
     gene            complement(946..3180)
                     /locus_tag="KRL67_003720"
     CDS             complement(946..3180)
                     /locus_tag="KRL67_003720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HBH1524552.1"
                     /translation="MQNHNYIKMLNKKVEVLSKELDSRNFKLKTLNEIDFFSKIPNRE
                     FMYQYIDDCISKNIHIGIMLLNIDNFKLFNDIYGYLVGENLLITLTQNLLHFIPESKG
                     IVAYLKGEGFLIVLPNVSNKELNNMGATIIESARELKIRLPNEKEIYSNVTFSIGSTI
                     WNGEPNMTTLMLFNQVYSALKKAKSEGKNRYILFNFKDNSFYNKLENDKEFLSEISYV
                     LQTKQIEVFYQPLYNIKNNTIVGCEALARWRHPIKGLLTPDKFIPLFEQFGLITELDL
                     HLFEENCKNIRKWIDKNDVFVPICCNFSRLHFFNPNFPSMLKGITEKYNVSTSNFSIE
                     ITENILIENTNTIIQQLTELRSYGFSVSMDDFGSGYSSFGMLQNLPFDIIKIDRIFLS
                     HNLNDFKNTTILYSIINIARALGMLTVCEGVETEQHVEFIKSINCDIAQGYYYAKPME
                     CKEFEKLLSKENNSEKMFIKYSDIGKSLIEKIFDGFFIMQDMKKLRANVTEDIQWFDF
                     YNKTEITSFPKVIEHFEKHIKGKKFSILYKSITPHNTESDSLLISGEAVLSDEAAKHQ
                     KYSNFYFSANCIISENKLLLAKLHLDLIKTAGYINEFESYSIPKMTNTHLDESNVLSQ
                     FYSMLPVGIIRYDLFGDMIITYINEEMFDIIGYTKEQFFGEMGGNLRAIVHPDDLDFV
                     YKKSLDMIDTNKIEPFLYRFIKRDGQIVTVMYTQCNLSAIDGRSIAQGMYIDINKIKN
                     LTKI"
BASE COUNT         1224 a          473 c          386 g         1400 t
ORIGIN      
        1 acatatttat aaactaatgt acaagttaaa aaaagaaaga taattttaat ttattttaaa
       61 ataacaatgt taataatcat ttttatcaaa ttttttcttg tttttctctt tgtttttata
      121 cattttttta tctaattcaa ctattgtcaa ttttaaattt ctttttactg tatcaacacc
      181 atagctaaat gaaagatatt tatacttaga gaatactttt aaaaaatttt ctcttaaggt
      241 ttctagttgt gaataaacat aatttttgtc aacatcacta gagaatataa caataaattc
      301 atctcctcct aatcttataa tagaggtaga atttattaat tctttattta aaagtgaaac
      361 aaaatcacat aaaatcttat ctcctacatt atgaccttca tagtcattta tcattttgaa
      421 attatctaag tcaattagta caatataatc tcctataaat atgtcaaatc tatcatttaa
      481 atattttcta ttatatgctc cagttaatgg gtcagtaatt gagtagtgaa gatttctctt
      541 attttcttca tagagttttg ttatatcatt ggccatagta attatgtagg gtttttcatc
      601 aacatctact ttgtattcta aaacattcca ataacttgta gtatcttttt taaactctaa
      661 aatccaattt gaaattttat cataaggata atttttttcc caatctaatt gaatattgtg
      721 agaatccgta aaaaatttag caatattatc agggagtttc ttagctattt ctatattagt
      781 cattccaagt ggattatctt gcatataact tttaattaaa ttttttaaat tatcatttat
      841 aaatataatt ctgtagttag aatctcttat aattagaaat cctggaaaag ttcttaaaaa
      901 aatttcttta aacatattaa tttctccaaa ataaaatact ttgtactaga tttttgttaa
      961 attttttatt ttatttatat ctatatacat accttgggca atcgaccttc catcaattgc
     1021 agaaaggtta cattgtgtat acataactgt gacaatttga ccatctcgtt ttatgaatct
     1081 atataaaaat ggttcaattt tatttgtgtc aatcatatcc aaactttttt tataaacaaa
     1141 gtctaaatca tctggatgaa caattgctct taaatttccg cccatctcac caaaaaactg
     1201 ttcttttgta taacctataa tatcaaacat ttcttcattt atataagtaa taatcatatc
     1261 accaaataaa tcatagcgaa ttatacccac aggcaacatt gaataaaatt gtgataaaac
     1321 attactttca tcaagatgtg tatttgtcat tttaggtatc gaatatgatt caaattcatt
     1381 tatatagcca gcagttttaa ttaaatccaa atgtaatttt gctaagagta atttattttc
     1441 tgaaattata caatttgcac taaagtagaa attactatat ttttgatgtt tagcagcttc
     1501 atctgataaa acagcttctc cactaattaa taatgaatca gattcagtat tatgtggggt
     1561 tatacttttg taaagaatag aaaatttttt acctttaata tgtttttcaa aatgttctat
     1621 tactttagga aaagatgtaa tttcagtttt gttgtaaaaa tcaaaccatt gaatgtcttc
     1681 agtaacatta gctcttagtt ttttcatatc ttgcataata aaaaatccat caaatatctt
     1741 ctctattaaa gattttccta tatcagaata ttttataaac attttctctg aattattctc
     1801 tttagatagt aatttttcaa attctttgca ctccataggt tttgcataat aataaccttg
     1861 ggcgatatca caatttatac ttttaataaa ttctacatgt tgctctgttt caacaccttc
     1921 acatactgta agcatcccaa gagctcttgc aatgtttatt atagagtaca aaatggttgt
     1981 atttttaaaa tcatttaaat tatgagataa aaatatccta tcaattttga ttatatcaaa
     2041 aggtagattt tgcaacatac caaaagatga atacccagaa ccaaaatcgt ccattgatac
     2101 agaaaaacca taagaacgaa gttcagttaa ttgttgaata attgtatttg tattttctat
     2161 taatatattt tctgttattt caatgctgaa atttgaggta gagacgttgt acttttcagt
     2221 aatacctttt agcatacttg gaaaattagg attaaaaaaa tgaagacgag aaaagttaca
     2281 acaaattgga acaaaaacat catttttatc aatccatttt cttatatttt tacagttctc
     2341 ttcaaataaa tgcaaatcta attccgttat cagaccaaac tgttcaaata aaggaataaa
     2401 tttatctgga gtaagaagtc ctttaatagg atgtctccat cgtgcaagcg cctcacatcc
     2461 aactatagta ttgtttttta tattataaag tggttggtaa aatacttcaa tttgtttagt
     2521 ttgtagaaca tatgatattt cggataagaa ctccttatca ttttctaatt tattgtaaaa
     2581 gctgttatct ttaaaattaa atagaatgta tctatttttg ccttcacttt tagccttttt
     2641 taaagcacta tatacttggt tgaaaagcat caaagtagtc atgtttggtt ccccattcca
     2701 gatagtgctg ccgatactaa atgtaacatt ggagtatatt tctttttcat taggaagtct
     2761 tatttttaat tctcgagcac tctctataat agttgcaccc atattattta actctttatt
     2821 tgatacattt ggaagtacaa ttaaaaaacc ttctcctttt aaataagcga ctattccctt
     2881 tgattctgga ataaagtgca ataggttttg agttagagta attaataaat tttctccgac
     2941 tagatatcca tatatatcat taaataattt aaagttatcg atatttaata acatgattcc
     3001 tatatgtata tttttcgaaa tacagtcatc aatatattgg tacataaatt ctctgtttgg
     3061 tattttcgaa aaaaaatcta tttcatttaa ggtttttaat ttaaaatttc tactatctaa
     3121 ttccttagat aatacttcta cctttttatt taacattttt atataattat gattttgcat
     3181 ggtattcctc cataagatac tttaaatttt gatgacaact gtatttatgc tagaagtatt
     3241 taaatgttaa tttgtaaata atttaaatgc taaattttaa ataagtattt aaaatgaata
     3301 tacattaagt tatttgctcc aatgctacaa atacaaatat tttttagttt agtaataata
     3361 ttagataaaa ctagttaatg cttaaattct agtatattga aagatttcct ttttgaatgc
     3421 aattatatca tattttttca taaatagata gattgtgagc tgtacagtta gtaaggtaga
     3481 gtt
//
LOCUS       DAEPXF010000279         3462 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain HB27
            SAMN13376389-rid12479423.denovo.287, whole genome shotgun sequence.
ACCESSION   DAEPXF010000279 DAEPXF010000000
VERSION     DAEPXF010000279.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13376389
            Sequence Read Archive: SRR10518432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3462)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3462)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001069624.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001069624.1
            Genome Coverage       :: 315x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/15/2021 01:54:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,165
            CDSs (total)                      :: 4,076
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 89
            rRNAs                             :: 10, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 10 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 19 of 69
            Pseudo Genes (incomplete)         :: 44 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 10 of 69
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3462
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13376389-rid12479423.denovo.287"
                     /strain="HB27"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="China: Hebei"
                     /collection_date="2017"
     gene            complement(137..1153)
                     /locus_tag="KRL67_003721"
     CDS             complement(137..1153)
                     /locus_tag="KRL67_003721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBH1524553.1"
                     /translation="MVTIKDIARNLGISYSTVSRCLNNNPNVSEKTKKKVVEEANRLG
                     FHFNVNARNLAKKETNRIGVIFSNNFNHQDTRKFFSDIMDSSINSIETNKYDFIIQPN
                     NNISGDSNVYKMVNGQMVDGLVIVSQSIKKEEYDFLKDNNFPHVFIYFKPSFIGEVDN
                     FFWDDNVYGGYIATKHLIDHGHKDIITVTSDDKSSKMHEDRTKGYLNAMEEANLKTEV
                     IKSKMDFESQVEFLEKYIDKIKKASAIFVQQDVPAISIIQELKTTYGINVPEDISIIG
                     YNNIELISYFRSHLSTIDDPREQVIKNGVDSLVNIINKKSTKHIPRKLYPRLIIRNSV
                     KRIK"
     gene            complement(1336..1698)
                     /gene="spoVAE"
                     /locus_tag="KRL67_003722"
     CDS             complement(1336..1698)
                     /gene="spoVAE"
                     /locus_tag="KRL67_003722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein AE"
                     /protein_id="HBH1524554.1"
                     /translation="MLMDYVRVFIVGGIICLIAQVMMDYFKMQTPYVMVTYVTTGVVL
                     TFLGLYEPLVKFAAAGATVPIIGFGYSLATGVIKSIQSDGFLGIFLGGTTATAGGIAA
                     AIFFGYIMSVVFTPKAKP"
     gene            complement(1712..2725)
                     /gene="spoVAD"
                     /locus_tag="KRL67_003723"
     CDS             complement(1712..2725)
                     /gene="spoVAD"
                     /locus_tag="KRL67_003723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein AD"
                     /protein_id="HBH1524555.1"
                     /translation="MKNKRIGKRTVKLENKPTIISTGTIVGPKEGEGPLKDYFDMIMT
                     DDLYGEKTWELAESKMVETASQKAIQKAGKKLSDVNYMLGGDLINQIVPASFAARELA
                     IPFLGIYGACSTMAEGLCIGSMLIDGGFADLVLSGTSSHYCTAERQFRFPLELGNQKP
                     MTAQWTVTGAGSVLLAPNGEGPKVKYVTVGKVIDEGIDDGNNMGPAMAPAAIDTIYSY
                     FEDTNDDPNSFDIIATGDLGTLGKQIAEDFLKEKGVDISKVYTDCGIEMFNLKEQDVH
                     CGGSGCGCSATVFAGYIYDKLRKKEFNKVMLVSTGALLSPTSTLQKQTIPCVAHAVVI
                     VNE"
     gene            complement(2892..3329)
                     /gene="spoVAC"
                     /locus_tag="KRL67_003724"
     CDS             complement(2892..3329)
                     /gene="spoVAC"
                     /locus_tag="KRL67_003724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein AC"
                     /protein_id="HBH1524556.1"
                     /translation="MDKNYKKYVDQISPKPTYLKNYTLAFIVGGIICMIGQAINDLYM
                     KVGGLDKLGASSATSITLIFIGAFLTGLGVYDLIGKRAGAGSIIPITGFANSIVSPAM
                     EYKREGYVLGVGANLFKIAGPVLVYGIGSSILCGIIYYIFKMF"
BASE COUNT         1119 a          636 c          371 g         1336 t
ORIGIN      
        1 aaaactatct aaatagacaa aggagtacaa tgtacttata tacacctact ccttttaatt
       61 tttatttata ttatttgtta aaaaacttca cataatctat aatattttta ataagctttt
      121 gatacttaaa agcttactac tttattcttt taacgctatt tcgtattatt aatcttggat
      181 ataattttct tggaatgtgt tttgtactct ttttatttat tatatttact aaactatcta
      241 ctccattttt tataacttgc tctcttgggt catctattgt tgataaatgc gacctaaaat
      301 atgatattaa ttcaatatta ttatacccta ttatagaaat atcttctggt acatttatac
      361 cataagtagt ttttaactct tgtattatag aaatcgctgg tacatcttgt tgtacaaata
      421 tagctgaagc tttttttatt ttatcaatgt atttttctaa aaattcaact tgagattcaa
      481 aatccatttt agatttaatg acttcagtct ttaaatttgc ctcttccata gcattcaaat
      541 atcccttggt tctatcttca tgcatttttg aactcttatc atctgaagtt actgttatta
      601 tatctttatg accatggtca attaaatgct tggtagctat ataacctcca tacacgttat
      661 cgtcccaaaa gaaattgtct acttctccta taaaagatgg tttaaaatat ataaatacat
      721 gtggaaaatt attgtctttt aaaaaatcat actcttcttt ttttattgat tgagacacaa
      781 ttactaatcc atcaaccatt tgtccattta ccattttata tacattacta tcaccactaa
      841 tgttattatt aggctgtatt ataaaatcgt atttattagt ctcaatagaa tttatagaac
      901 tatccattat atcactaaaa aactttctag tatcttgatg attaaaatta tttgaaaaga
      961 ttacccctat cctgttagtt tctttttttg ctaaatttct tgcatttaca ttaaaatgga
     1021 accctagtct atttgcctct tctactactt tcttttttgt tttttcagat acattaggat
     1081 tattgtttaa gcacctagaa acggttgaat aactgatacc tagattcctt gcaatatctt
     1141 taatagttac catctttatc ccctacttca cttcatttta ttttgataat ataatactac
     1201 actatgttgt aaataattgt caacttcaat tatgtaaacg cttctttata taaaaaatag
     1261 gtggaatcta aaagatatcc acctgcttta taaaaagtta ttatttcaaa aacttactat
     1321 gctcttaata aagatttatg gttttgcttt tggagtaaat actactgaca taatatatcc
     1381 aaagaatata gctgctgcta ttccaccagc tgtagcagtt gtacctccta gaaaaatgcc
     1441 taaaaaacca tcactttgta ttgactttat tactcctgta gcaagtgaat atccaaatcc
     1501 tattatagga actgttgcac ctgctgctgc aaacttaact aatggctcat aaagtcctag
     1561 aaaagttaaa actacaccag ttgttacata cgttaccata acatatggag tttgcatttt
     1621 aaaataatcc atcattactt gggctattaa acatataatt ccacctacaa taaatactct
     1681 tacatagtcc attaacatat tatatcactt cctactcatt tactattact actgcatgtg
     1741 caacacatgg tattgtctgc ttttgtagtg tacttgtcgg tgaaagtagt gctcctgttg
     1801 atacaagcat tactttgtta aattcttttt ttctcaactt atcatatata tatcctgcaa
     1861 acactgtagc tgaacaccca catccacttc caccacagtg gacatcttgt tctttaaggt
     1921 taaacatctc tataccacag tcagtgtaaa ctttcgatat atctacacct ttttctttta
     1981 aaaagtcttc tgctatctgt tttccaagag tacctaaatc tccagtagca attatatcaa
     2041 agctatttgg gtcatcatta gtatcttcaa aataagaata gatggtatct attgctgctg
     2101 gagccatagc tggtcccata ttattaccat catcaatacc ctcatctatt actttaccaa
     2161 ctgtaacata ctttactttt ggaccttctc cattaggagc taataataca ctccctgctc
     2221 ctgtaactgt ccactgagct gtcattggtt tttgatttcc aagttcaagt gggaatctaa
     2281 attgtctttc tgctgtacaa tagtgacttg atgtacctga cagtactaaa tcagcaaaac
     2341 caccatctat aagcatagaa cctatacaaa gaccttctgc cattgtagaa caagctccat
     2401 atatacctaa aaatggaata gccaattctc ttgctgcaaa tgatgctggc actatttgat
     2461 ttattaaatc tccacctaac atataattta catctgacaa ttttttacct gccttttgta
     2521 tagctttctg agatgcagtc tctaccattt tactttcagc taattcccaa gtcttttcac
     2581 catataaatc gtcggtcata atcatatcaa aataatcctt tagtggtcct tcaccttcct
     2641 taggacctac tatagttcca gttgatatta ttgttggttt attttctaac ttgactgttc
     2701 tttttccaat tcttttattt ttcatatttt cacacctttt taattttgca ttttactatt
     2761 caaattttta atattaatta aaaatattaa ttaaatctgc acaattattt ttcaattagt
     2821 tactttagta gctatcttca gattttataa cttcttcata attaataaaa tttaaattaa
     2881 ataaaaatca tctaaaacat cttaaaaata taataaataa ttccacataa aatagaagaa
     2941 cctataccat aaactaaaac tggtcctgca attttaaata gatttgcacc tacacctaat
     3001 acatagcctt ctcttttata ctccattgct ggagatacta ttgagttagc aaaacctgtt
     3061 attggaatta tagaaccagc tccagctctt tttcctatta ggtcgtaaac tcctaatcca
     3121 gttaaaaatg cccctataaa tattaatgtt attgatgttg cagaactagc acctagttta
     3181 tcaagacctc caactttcat atacaaatca tttattgctt gccctatcat acaaataatc
     3241 ccacctacaa taaatgcaag tgtataattt tttaaatatg taggttttgg gcttatttgg
     3301 tctacatatt ttttataatt tttatccata ctaaacacct ctctaaagta ttatttgtta
     3361 attttaaaaa aataaaccta ttatttattt tgactatcta aagtttggtc aaaaaataaa
     3421 aaagatgcct aataaagaaa aaatctattt ttccataaag ac
//
LOCUS       DAEPXF010000280         3417 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain HB27
            SAMN13376389-rid12479423.denovo.288, whole genome shotgun sequence.
ACCESSION   DAEPXF010000280 DAEPXF010000000
VERSION     DAEPXF010000280.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13376389
            Sequence Read Archive: SRR10518432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3417)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3417)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001069624.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001069624.1
            Genome Coverage       :: 315x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/15/2021 01:54:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,165
            CDSs (total)                      :: 4,076
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 89
            rRNAs                             :: 10, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 10 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 19 of 69
            Pseudo Genes (incomplete)         :: 44 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 10 of 69
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3417
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13376389-rid12479423.denovo.288"
                     /strain="HB27"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="China: Hebei"
                     /collection_date="2017"
     gene            complement(<1..180)
                     /locus_tag="KRL67_003725"
     CDS             complement(<1..180)
                     /locus_tag="KRL67_003725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH1524557.1"
                     /translation="MERLMELIERKFLKDVELLELEDIENVIECKMTQPYNAHYNEYL
                     VKVKNGDEVEEYFVYL"
     gene            431..679
                     /locus_tag="KRL67_003726"
     CDS             431..679
                     /locus_tag="KRL67_003726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBH1524558.1"
                     /translation="MCNNLQLNKSYFCSLFKKESGYTFTSFSNKIRVEKRKKYLLKKD
                     LSILDVAVLVGFNSQNYYSMVFKKFNNLTPIEYKNIYN"
     gene            988..2607
                     /locus_tag="KRL67_003727"
     CDS             988..2607
                     /locus_tag="KRL67_003727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HBH1524559.1"
                     /translation="MNIVISLIGGLGLFLYGMSLMGEGLQKSAGDKLKKIIELLTSNV
                     VMGVLVGTVVTGIIQSSSATTVMVVGFVNAGIMNLSQAIGVIMGANIGTTVTAQLVSF
                     NLEGIAPIALGIGILFYLFTSNQKTKHLSEILIGFGILFTGMEFMKDAVAPLAEYKAF
                     TDALLYFSKNPVLGILAGFAITGIVQSSSASMGMLIALASQGILPLSSALPILYGDNI
                     GTCVTSLLSSVGASRNARRAAVMHLSFNVIGTIIFMLVLNKPISAIVTHFDPTDTARQ
                     IANAHTLFNLTNVIILLPFSKYIVKLANRLIPIKETESEIVNNTKYLDERMFSTPSIA
                     LGNTVQEVVRMGHKATNSLEHSIAGFLNKSNEDINKAFESEKVVNKLQKDILNYLLKL
                     SKEPLRDDERFRTDLLFNTVNDIERVSDHAENIAELAMSVKEMNISFSDSAIREIYEI
                     YNKTITNFKDALIVLDVKDFELANKVLEVENEVNYLEKTFRNSHMIRLNNGSCTIDAG
                     VLYLDLLTNLERISDHSTNIVKQVLKLKQKI"
     gene            2981..3175
                     /locus_tag="KRL67_003728"
     CDS             2981..3175
                     /locus_tag="KRL67_003728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HBH1524560.1"
                     /translation="MINYMVEIIYSEKISEVENMLKIENIQYEFTSRDGFIFFGFYEM
                     TDAYRFETLCSDEKIRFKRI"
BASE COUNT         1285 a          405 c          444 g         1283 t
ORIGIN      
        1 taaatacaca aaatattctt ctacttcatc tccatttttt acttttacta agtattcatt
       61 ataatgagca ttatatggct gtgtcatttt gcattctatc acattttcta tgtcttctaa
      121 ttctaaaagt tctacatcct ttaaaaattt tctttctatg agttccatta atctttccat
      181 aatatatcct cacttcccct ataattaaga taaactatat atcttcttaa tacagagttt
      241 atctgtttat tatattatat ccaatatatt acagtttcta acataattca taaattattt
      301 aacaaataga gattgatttt ttaataaata tgccttgata aaatgaaaca tagaccaact
      361 cttagtttta tgtaaaaaaa ctatagatta tattcacaaa aattactcat aagatataac
      421 agtaagtaat atatgtaata acttacaact taataaaagt tatttttgtt ctttgtttaa
      481 gaaagaaagt ggatatactt ttactagttt ttcaaataaa attagggttg aaaaaaggaa
      541 aaagtatcta ttaaaaaaag acttgtctat tttagacgtt gctgtactag ttggattcaa
      601 tagtcaaaat tactatagta tggtttttaa aaagtttaat aaccttacac caattgaata
      661 taaaaatatt tataactaaa aaatatcatt ctaaaaggat gctatttttt tacatatttt
      721 ttacaattat agttaattct ttgttaaatt gattgtacat aaaatactaa aaatctaagt
      781 tcaagtattt aaattgctat atatcataaa ttttctaaaa ttaacaacaa cttaaaaatt
      841 taacattctc ttaacgtata cttaacaaaa cattaatatt tttattgaca aaatttgtta
      901 ttctatatta tggaaacaat aaattttagt taataaagtt tataattcag ataaaaaaat
      961 tattaatttc ttaagggagg aaatatcgtg aatatagtaa ttagccttat cggtggactt
     1021 ggactttttc tttacggaat gagtttaatg ggagaaggtt tacaaaaatc agcaggagac
     1081 aaattaaaaa aaattattga actcttaaca agtaatgttg ttatgggtgt actggtaggt
     1141 acagtcgtta caggtattat acaaagttca agtgctacta ctgttatggt tgtaggattt
     1201 gtaaatgcag gtataatgaa tctaagtcaa gctattggtg taataatggg tgcaaatata
     1261 ggtactacag ttactgctca attagtttca tttaatcttg aaggaattgc accaattgct
     1321 ttaggtatag gtattttatt ctacttattt acatcaaatc aaaaaacaaa gcatttatct
     1381 gaaatactta taggttttgg tatattattt acaggaatgg agtttatgaa agatgcagta
     1441 gctccacttg ctgaatataa agcattcact gatgcacttt tatattttag taaaaatcct
     1501 gtattaggta tattggcagg atttgccata actggtattg ttcaaagttc aagtgcatct
     1561 atgggtatgt taatagcact agcttctcaa ggaatacttc ctctatcatc tgctttacct
     1621 attctttatg gagataatat aggtacttgt gttacttcac ttttatcaag tgttggtgca
     1681 agtagaaatg ctcgacgtgc agctgtaatg catttaagtt ttaatgtaat tggtacaata
     1741 atatttatgc ttgttttaaa taaacctata tcagccatag ttactcattt tgacccaact
     1801 gatacagcta gacaaatagc aaatgcacat accttattta atcttacaaa tgttataata
     1861 ttattaccat tttctaaata catagttaaa ttagcaaata gacttatacc tattaaagaa
     1921 actgaatcag aaatagtaaa taatactaag tacttagatg aaaggatgtt tagcactcca
     1981 tctatagcac ttggtaatac tgtacaagaa gttgtaagaa tgggtcataa agctacaaac
     2041 tctttggaac attcaattgc aggattttta aataagagca atgaagatat taataaagct
     2101 tttgagtcag aaaaagttgt caataagtta cagaaagata tattgaacta tttgttaaaa
     2161 ctttctaaag aacctcttag agatgatgaa cgctttagaa cagatttact ttttaatact
     2221 gtaaatgaca ttgaaagagt ctctgaccat gcagaaaata tagctgaatt ggccatgagt
     2281 gttaaggaaa tgaatattag tttttctgat agtgcgataa gagaaatata cgaaatatac
     2341 aataagacaa ttactaattt taaggatgcc ttaatagttt tagatgttaa agattttgag
     2401 ttagcaaata aagttttaga agttgaaaat gaagtaaatt atttagaaaa aacttttaga
     2461 aattcacata tgattagact aaataatgga agttgtacaa ttgatgctgg tgtactttat
     2521 cttgacttac ttactaattt agaaagaatt tctgaccact caacaaatat agtaaaacaa
     2581 gtattaaaac ttaaacaaaa aatatagtga ttatttttat atataatctt aatcaatgag
     2641 aaacaagaag ctatagataa tgcttatatc atttatagct tcttattttt aatgtaatac
     2701 tcaaattttt atagtattct cttattatta aacagtttct gcctagaata ttccatatat
     2761 atatgacttc tttcataaaa cttgaactgc ccataactaa agtcacaagt atacatagct
     2821 atttattaat caattataac caatatttat tttatttatc ttcattttaa ttcttcattc
     2881 aataatgtaa taagattatt ttttatttgg atactattat atcatctaaa tatgttatta
     2941 tatatttgga tattttcata ttaataggag ggattctaca atgataaatt atatggtaga
     3001 aattatatat agcgaaaaaa taagtgaagt agaaaacatg ttgaaaattg aaaatataca
     3061 atatgaattt acttctcgtg atggctttat attttttggt ttttatgaga tgacagacgc
     3121 atatagattt gagactttat gttcagatga aaaaattcga tttaaaagaa tataatagat
     3181 aaaaaaagta catatttcta aactatttgt ttttcaaatt taggttgtgt tttaatgtag
     3241 tgttgatatt ataaacaatt ttaataataa aaaagtaggg acattatatt aatatgctcc
     3301 tactttttac tcatacttat taatattatt tatcaaaatt accctacaaa ctggaattta
     3361 tcattctttc ataattttaa actatcatat ttaagatata taaaattcta catgatt
//
LOCUS       DAEPXF010000281         3409 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain HB27
            SAMN13376389-rid12479423.denovo.289, whole genome shotgun sequence.
ACCESSION   DAEPXF010000281 DAEPXF010000000
VERSION     DAEPXF010000281.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13376389
            Sequence Read Archive: SRR10518432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3409)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3409)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001069624.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001069624.1
            Genome Coverage       :: 315x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/15/2021 01:54:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,165
            CDSs (total)                      :: 4,076
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 89
            rRNAs                             :: 10, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 10 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 19 of 69
            Pseudo Genes (incomplete)         :: 44 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 10 of 69
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3409
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13376389-rid12479423.denovo.289"
                     /strain="HB27"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="China: Hebei"
                     /collection_date="2017"
     gene            56..1033
                     /locus_tag="KRL67_003729"
     CDS             56..1033
                     /locus_tag="KRL67_003729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HBH1524561.1"
                     /translation="MSLVNNQYKGKIKSANLSVDYEINQVMKDIDKLGLNTVNVPIII
                     NVESINSDIMSIDNNSKAKAIKLIKKLNKKGISTILEAYPWIENGKLYETDWNPINKK
                     RFFYTWQNTILNELIIDVANPLDVNVLNIGSNFVHLEGYQQNWGEIIDFVQSKFDGLV
                     TYRTNWWYTKKDDLSSKLFYEQKLNNNFFDKLDFISIAAYFELSNKSVNTVDELISAL
                     HSSTVNNREQNIKQEIYNLYKAHRKPIFFGELGFSNRESASSQPWNHAPSKVVNGEEQ
                     ARCFEAYKQVFENEDWINGFSVFCVGKIDDEKNFYPSKESIKVIKSWYE"
     gene            1087..2211
                     /gene="wecB"
                     /locus_tag="KRL67_003730"
     CDS             1087..2211
                     /gene="wecB"
                     /locus_tag="KRL67_003730"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HBH1524562.1"
                     /translation="MNDIKVMTVFGTRPEAIKVAPLIKELEKRENIKSIVCVTAQHRE
                     MLDQVIETFNINVDYDLDIMEKGQSLNDITCKILNKLPLILNKENPNIILVHGDTTTT
                     LATSLTAFYNKTLVGHIEAGLRTYDKYSPFPEELNRQLTGIIADMHFAPTNLAKKNLI
                     SEGKPNNNIFVTGNTAIDALKMTIKENYNHPIIDEIGNDRMILLTSHRRENLGKPMKN
                     IFRAIKRIVDDFEDVQIVYPIHLNPKIRTIADEIFGKFPKKIHIIEPLDVADFHNFLN
                     KSYMIMTDSGGIQEEAPSLGKPVLVLRDKTERTEGIEAKTLKLVGTNEDRIYNSVSDL
                     LINKDNYVQMSKASNPYGDGNASKYIVDIIIKKFNCKYLN"
     gene            2287..3261
                     /locus_tag="KRL67_003731"
     CDS             2287..3261
                     /locus_tag="KRL67_003731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HBH1524563.1"
                     /translation="MARKLIKNLGKSKSVKRVKLLFKKIFITVFIVASIVAIFNITKY
                     FEELYKVRDLKSTKIEYYMDVADEAGDGKVQLSWKALLAIDMVIHDEDLSNIKKKDTL
                     NIGEKFIVEDKNDKGEKVYKVKKFNKVLSELKFDSSQKSRARKYMKDLEYTYLGNKQL
                     DSSDEKIKFIKQLEDSAIREYIDYGILPSITIGQAILESGWGNSKLTKQSNNLFGIKA
                     DKAWKGKSVEISTSEHYNEKIVASFRSYNSLQDSVKDHSLFLINNKRYRKHGLFEAKD
                     YISQAQALENAGYSTAEDKKGNRIYAELLIDVIRSYNLQLIDNKVETK"
BASE COUNT         1433 a          331 c          588 g         1057 t
ORIGIN      
        1 aataatctta atttttatat ttataattta ctttgtaagt attgagctaa aagatatgag
       61 tctagtaaat aatcaatata aaggtaagat taaatcggcc aatttatctg tagattatga
      121 aataaatcaa gttatgaagg atatagataa attgggattg aatacagtaa atgtacctat
      181 aattataaat gtagagtcca ttaattctga tattatgtca atagataata atagtaaagc
      241 aaaggcaatt aagttaataa aaaaattaaa taaaaaaggt atttcaacaa tattagaggc
      301 atatccatgg attgaaaatg gaaaattgta tgagactgac tggaacccta taaataaaaa
      361 aagatttttt tatacttggc aaaatactat attaaatgaa cttataattg atgtagcaaa
      421 tccactggat gtaaatgttt taaatattgg ttctaatttt gtacatttgg aaggatatca
      481 acaaaattgg ggtgaaataa tagattttgt acaatccaaa tttgatggtc ttgtaacata
      541 cagaactaat tggtggtata caaaaaaaga tgacttaagt agtaagttgt tttatgaaca
      601 aaagttgaac aataattttt ttgataagtt agattttata tctatagcag catattttga
      661 gttaagtaat aaatctgtta atacagtaga tgaattaatt tctgccttac actcatctac
      721 tgtaaacaat agagaacaaa atataaaaca agaaatatat aacctttata aagctcatag
      781 aaaacctata ttttttggag aattaggttt ttcaaataga gaaagtgcaa gttctcagcc
      841 gtggaatcat gctccttcaa aagttgtaaa tggcgaagaa caagcaaggt gttttgaggc
      901 atataagcaa gtttttgaaa atgaagactg gataaatggt ttttctgtgt tttgtgtagg
      961 taaaatagat gacgaaaaaa atttttaccc atcaaaagag agtataaaag ttattaagtc
     1021 ttggtatgaa taattttgaa agtgtagaat ttatatagat taaatagacg attaggagag
     1081 atataaatga atgacatcaa ggttatgact gtatttggaa ctagacctga agctatcaaa
     1141 gtagcacctt taataaagga acttgaaaaa agagagaata taaaaagtat tgtttgcgtt
     1201 acagctcaac atagagaaat gcttgaccaa gttatagaaa cctttaatat aaatgtagac
     1261 tatgatttgg atataatgga aaaaggacaa agcctgaatg atataacatg taagatttta
     1321 aataaacttc ccttaatttt aaacaaggaa aatccaaata taatattggt acatggagat
     1381 acaaccacaa ctctagctac aagtttgact gcattttata ataaaactct tgttggtcat
     1441 atagaagctg ggcttagaac ctatgataaa tattctcctt ttccagaaga gttaaataga
     1501 caacttacag gtattattgc tgacatgcac tttgctccta ctaatttggc taaaaaaaac
     1561 ttgatttctg aaggaaaacc taataataat atatttgtaa ctggtaatac tgctattgat
     1621 gctttaaaga tgacaataaa agaaaactat aatcatccta taattgatga gattggcaat
     1681 gataggatga ttttgttaac ctctcacaga agagaaaatt tgggtaaacc tatgaaaaat
     1741 atattcagag ctataaagcg aatagtagat gattttgaag atgtacagat agtatatccc
     1801 atacatttaa atcctaaaat acgaactata gctgatgaga tatttggaaa atttcctaaa
     1861 aaaattcata ttatagagcc tttagatgta gctgattttc ataacttttt aaataaatct
     1921 tatatgataa tgactgatag tggaggaata caggaagaag ctccatcatt aggtaaacca
     1981 gttcttgtgt tgcgtgataa aacagaaaga acagaaggaa ttgaagcaaa gacacttaaa
     2041 ttagtaggta ctaatgaaga tagaatatat aattcagtga gtgatttatt aataaataaa
     2101 gataactatg ttcagatgag taaagcatct aatccatatg gagatggaaa tgcaagcaaa
     2161 tatatagtgg atataataat taaaaaattt aattgtaaat atttaaatta gataaggtct
     2221 aaaacattaa ctaatgatat aagatttatg ataatataag gaatacaatt tttaaggggg
     2281 agtaatatgg ctagaaaatt aataaaaaat ttaggtaaaa gtaaaagtgt aaagagagtg
     2341 aaacttttat ttaagaagat ttttattact gtattcatag tagcaagtat agttgctatt
     2401 tttaatataa ctaaatattt tgaggaatta tacaaagtaa gggatttaaa aagtacaaaa
     2461 attgaatact atatggatgt ggctgatgaa gcaggggatg gaaaggtcca attaagttgg
     2521 aaggccttac ttgctataga catggtaata catgatgaag atttaagtaa tataaaaaag
     2581 aaagacacat tgaatatagg ggaaaagttt atagtagaag ataaaaatga taaaggcgaa
     2641 aaggtgtata aggtaaaaaa gtttaataag gtattaagcg aattgaaatt tgactcttct
     2701 caaaaaagta gagcaagaaa atacatgaaa gatttggaat acacatatct tggaaataaa
     2761 caattagata gtagtgatga aaaaattaaa tttataaagc agttagaaga ctcagctata
     2821 agagaatata ttgattatgg aatattgcca tctataacaa ttggacaagc tatattagaa
     2881 tctggctggg gaaattctaa acttacaaaa cagagtaata atttatttgg tataaaagca
     2941 gataaagcat ggaaaggaaa aagtgtagaa atttcaactt cagagcatta taatgaaaaa
     3001 attgtagcta gttttaggtc ttataattca ttacaagatt ctgtcaaaga ccacagttta
     3061 tttttaatta ataataaaag atatagaaaa catgggttgt ttgaggcaaa agattatatt
     3121 agtcaagctc aagcattaga aaatgcagga tatagtacag cagaagataa aaaaggaaat
     3181 cgtatatatg cagagctatt aatagatgtt attaggagtt ataatttgca attgatagac
     3241 aataaagttg agacaaaata attgttaact ttatatgagg agataacaag ataagattat
     3301 tttctacaaa aagaatagaa aaaatgtaaa aaaaactaaa aaaatgtatt gacttgttat
     3361 ttaaaaggtg atattattat tactgtactc gaaagagaac aaaatgaac
//
LOCUS       DAEPXF010000282         3401 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain HB27
            SAMN13376389-rid12479423.denovo.290, whole genome shotgun sequence.
ACCESSION   DAEPXF010000282 DAEPXF010000000
VERSION     DAEPXF010000282.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13376389
            Sequence Read Archive: SRR10518432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3401)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3401)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001069624.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001069624.1
            Genome Coverage       :: 315x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/15/2021 01:54:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,165
            CDSs (total)                      :: 4,076
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 89
            rRNAs                             :: 10, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 10 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 19 of 69
            Pseudo Genes (incomplete)         :: 44 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 10 of 69
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3401
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13376389-rid12479423.denovo.290"
                     /strain="HB27"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="China: Hebei"
                     /collection_date="2017"
     gene            124..462
                     /locus_tag="KRL67_003732"
     CDS             124..462
                     /locus_tag="KRL67_003732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HBH1524564.1"
                     /translation="MLIVTTEKVEGKKISKVLGLVRGSTIRAKHVGKDIGASFKNLVG
                     GELTGYNEMLTEARQIAIGRMVEDAEAKGANAVIAFRLSSASVMQGAAEMLAYGTAVV
                     LEDDNSILEK"
     gene            928..1431
                     /locus_tag="KRL67_003733"
     CDS             928..1431
                     /locus_tag="KRL67_003733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MogA/MoaB family molybdenum cofactor
                     biosynthesis protein"
                     /protein_id="HBH1524565.1"
                     /translation="MFNVAIITLSDKGYEGKREDITGKKLTEFVENTGAYKVTEYVLI
                     KDDKEMLKENITRLCNSNKIDLILTNGGTGFSKRDITPEATKEVLEKEIPGLSEYMRM
                     KSTEITKKAILSRGVSGIRNNSIIINLPGSPKGAVENLSFIIDVLDHGIEILRGEATE
                     CATKKSE"
     gene            1478..2437
                     /gene="moaA"
                     /locus_tag="KRL67_003734"
     CDS             1478..2437
                     /gene="moaA"
                     /locus_tag="KRL67_003734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HBH1524566.1"
                     /translation="MIDKYGRKIDYLRISLTDKCNLRCVYCMPPDVRFDKNYINENLS
                     FEDYKFIIKAMAEQGITKIRFTGGEPLLYPKLNELIKFTKEECGINNIGMTTNAIGLS
                     DRIFELEKCGLNKVNISLDSLKEYKYKSVTRGGNLKDVLKSIESCLNLGIKVKLNCVA
                     ISGFNDDELKDFMFLTVKSPIDVRFIELMPLGEANRVYKKGYFNVKEMIEKIYGLYKV
                     KNDEKSTAEYYMFKGAKGRIGIITPLSCSFCNTCNRIRLTSSGAIKLCLHSKEEIDIK
                     PFLHKPLLFRESMKDIIKQKPEKHHLIENKCSDTNRRMYEIGG"
     gene            2441..2911
                     /gene="moaC"
                     /locus_tag="KRL67_003735"
     CDS             2441..2911
                     /gene="moaC"
                     /locus_tag="KRL67_003735"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HBH1524567.1"
                     /translation="MLTHINEKGYAKMVDISEKDDTKRTAVATATITLSKEALEKVKN
                     GQIKKGDVLSVAQVAGIMGAKNTSSNIPMCHQIKLVGCDLEFDIDDSISAIKIYATCK
                     SLGKTGVEMEALSACSLAALTIYDMCKAVDKKMVISNIHLVEKTGGKSGDFKFE"
     gene            2947..3378
                     /locus_tag="KRL67_003736"
     CDS             2947..3378
                     /locus_tag="KRL67_003736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MOSC domain-containing protein"
                     /protein_id="HBH1524568.1"
                     /translation="MAKVVSINISEKKGVIKVPVQCAELKINHGIVGDAHAGNWHRQI
                     SLLGKESIDKMKMKGFDQLKYGDFAENITTEGIEVFSLPVGTKLRIGQCEVEVTQIGK
                     KCHNGCEIKKLTGDCVMPREGIFVKVIKEGIINVNDSIDVI"
BASE COUNT         1406 a          296 c          670 g         1029 t
ORIGIN      
        1 agtatagatt ggttaataaa agtatagatt ggttagtgaa acattaaatt acaatatttg
       61 ttaaaaaaat tgtgagttac atgatataat gtattaaata ggatactaag gagttgatat
      121 attatgttaa tagtaactac agaaaaagta gaaggtaaaa agatatcaaa ggttttagga
      181 ttagtgagag gaagtacaat aagagcaaaa catgttggaa aagatatagg agcaagtttt
      241 aagaatcttg ttggaggaga acttactgga tataatgaaa tgctcactga agcaagacaa
      301 attgccatag gtagaatggt tgaagatgca gaagctaaag gtgcaaacgc agtaatagca
      361 tttagactgt cttcagcttc agttatgcaa ggagcagcag aaatgcttgc ttatggaaca
      421 gcagttgttt tagaagatga taatagtatt cttgaaaaat aaaatataaa tagttaaaaa
      481 gaggcatctt ttaaaaattt tatggatgcc tttttttatt tgaaaaaact gatttgagaa
      541 gaaattttaa ttattatgac aataaaatta gggtgatatt atataaataa gtaaaattat
      601 attatataat gtatggtata gactttaaaa attttatttg tagagaaaaa tatggacaat
      661 gttataataa ttatagaaat taaaagtact tacattcgtt atgtatgtga catgtataat
      721 tagttttata tttaatacag ttttgaagta atatcttcat tttgagaacg ttggaataat
      781 aaaaatgtat gtagtatagg tgatataaat aaaaaataaa tacaatatgt tatattgctg
      841 atagaaaaga gaataaaaga aagtaggttt tattaaaaga gatttttatt tataggactt
      901 aatcaatgta tagaggggtg agaacgtatg tttaatgttg caattataac acttagtgat
      961 aaaggatatg aaggaaaaag agaggatata acaggaaaaa aattaactga atttgttgaa
     1021 aatacagggg cttataaagt tactgaatat gtacttataa aagatgataa agaaatgcta
     1081 aaggaaaata ttacaagact ttgtaatagt aataaaatag atttaattct tacaaatgga
     1141 ggtactggtt tttcaaaaag agatataacc ccagaagcaa ctaaggaagt gttagaaaaa
     1201 gaaataccag gtctgagtga atatatgaga atgaaatcca cagaaattac taaaaaggcc
     1261 atactaagta ggggtgtgag tggaataaga aataattcta taataataaa tttacctgga
     1321 agtcctaaag gtgcagttga aaatctaagt tttataattg atgttttgga ccatggcata
     1381 gaaattttaa gaggagaagc tacagaatgt gccactaaaa aaagtgaata gtatataatc
     1441 tagatagata aaaaatagac aaagaggtag ataaagtatg attgataagt atggaagaaa
     1501 aatagactac cttcgtattt cacttacaga taagtgtaat ttaaggtgtg tctattgtat
     1561 gccaccagat gtaagatttg ataaaaatta tataaatgaa aatctaagct ttgaagatta
     1621 caagtttatt ataaaggcta tggctgaaca aggtataaca aaaattagat ttactggagg
     1681 agaacctctt ttatatccta aattaaatga gcttataaaa tttacaaaag aagaatgtgg
     1741 aataaataat ataggaatga ccactaatgc tattgggctt tctgatagaa tctttgagct
     1801 tgaaaaatgt ggcttaaata aagttaatat aagtttagat tctttgaagg aatataagta
     1861 taaatccgta acaaggggag gaaacttaaa agatgtctta aaatcgattg aatcttgttt
     1921 aaatttaggt ataaaagtaa agttaaattg cgttgctata agtgggttta atgatgatga
     1981 gcttaaagat tttatgtttt taacagttaa atcaccaatt gatgtaagat ttatagagct
     2041 tatgccatta ggtgaggcta atagagttta taagaagggg tattttaatg taaaagaaat
     2101 gattgaaaaa atatatggtt tatataaagt caaaaatgat gaaaaaagta cagcagaata
     2161 ttatatgttt aaaggagcta aaggaagaat aggaattata actccattaa gctgttcatt
     2221 ttgtaacact tgtaatcgta ttagacttac ttctagtggt gcaataaaat tatgcctaca
     2281 ttcaaaagaa gaaatagata taaaaccatt tttacataaa ccattgttat ttagagaatc
     2341 tatgaaagat ataataaagc aaaaaccaga aaaacatcat ttaatagaaa ataaatgtag
     2401 tgatacaaat aggcgtatgt atgaaattgg aggataagta atgttaacac atataaatga
     2461 aaaagggtat gccaagatgg tggacataag tgaaaaagat gatactaaaa gaacggctgt
     2521 agctacagct acaattactc ttagtaagga agctttagaa aaagtaaaaa atggtcagat
     2581 aaaaaaagga gatgtattgt ctgtagctca agttgcaggt ataatgggag ctaaaaatac
     2641 atcttcaaac ataccaatgt gccatcagat taaattggtt ggatgtgatt tagaatttga
     2701 tatagatgat tctatatctg ctattaagat atacgcaacc tgtaaatctc ttgggaaaac
     2761 tggagtagaa atggaagctt taagtgcatg ttctttagca gcacttacaa tttatgatat
     2821 gtgtaaagca gttgataaaa aaatggttat aagtaatatc catcttgtgg agaagactgg
     2881 tggaaaatca ggagatttta agttcgagta aattaataat atattgggtt aaaatttgga
     2941 ggaaatatgg cgaaggtagt gtcaataaat ataagtgaga aaaagggtgt aattaaagtt
     3001 cctgtacaat gtgcagagct aaaaataaat catgggattg ttggagatgc acatgcagga
     3061 aactggcatc gtcagataag cttacttgga aaagaaagca tagataaaat gaaaatgaaa
     3121 ggctttgacc aattaaaata tggagatttt gctgaaaata taactacaga agggatagaa
     3181 gttttttcct tgcctgttgg tacgaagtta agaatagggc aatgtgaagt tgaagtaact
     3241 caaataggga aaaaatgtca caatggatgt gaaattaaga aattgacagg tgactgtgta
     3301 atgccacgag aaggaatttt tgtaaaagtt ataaaggaag gcattataaa tgtgaatgat
     3361 tcaatagatg taatctaatg aagtcactat taaaatataa t
//
LOCUS       DAEPXF010000283         3282 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain HB27
            SAMN13376389-rid12479423.denovo.291, whole genome shotgun sequence.
ACCESSION   DAEPXF010000283 DAEPXF010000000
VERSION     DAEPXF010000283.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13376389
            Sequence Read Archive: SRR10518432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3282)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3282)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001069624.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001069624.1
            Genome Coverage       :: 315x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/15/2021 01:54:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,165
            CDSs (total)                      :: 4,076
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 89
            rRNAs                             :: 10, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 10 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 19 of 69
            Pseudo Genes (incomplete)         :: 44 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 10 of 69
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3282
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13376389-rid12479423.denovo.291"
                     /strain="HB27"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="China: Hebei"
                     /collection_date="2017"
     gene            complement(<1..2039)
                     /locus_tag="KRL67_003737"
     CDS             complement(<1..2039)
                     /locus_tag="KRL67_003737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis cyclic di-GMP-binding
                     regulatory protein BcsB"
                     /protein_id="HBH1524569.1"
                     /translation="MKKFIISIISLVLFFSNISLIYKVNADETKVKNYKFERDITIDG
                     VIGSNSTFFEVNKNWDIEEVLLHLNFSKSQILNGDVSSLTVLINNVPIKSIKLNAKTN
                     YKNTLEVLVPKDYVIQGYNEIKIKTYKTISDKICQDDSNTGNWMVIHKESYISIRYKQ
                     KKMGNSINEYPYPYAEIENNHKLDTTIVVPDNMTRGETTAVFNLASAFGKITKNDDLK
                     LDVKLYSEMKNWSDDNIIYIGKPENTAEEILDILSTKEQTLLSSNCIIKQVDSPYNKN
                     KKMMVVIGSNEDDLIKASNLLIENRLSNQVLSSSVFVNKETNIKINREQKLNLGHLTL
                     KDLGYSDFLLEGAFNQQALFDVKIPTGKVLDDGSKIILNLRYSDNLDFEKSLVTVSIN
                     DVIVGSKKLDRSHSNNDKLELKIPKDIDNKNYYQVKLTFNLSIKNSNCVTRESNNPWA
                     YVSNNSYLALSTKENETLSFENYPYPFVRDDEFNDLTVIMPDYSGSQAMTWMFRLGVT
                     LGANINSHNGNINVIRGKEFSDKYKDTNIVVFGVPHNNSVIKMLNNNLNIKFDKNYSD
                     FISNDKISFIDDYGKNISTIQLIKSPYNNQKNIMVISSMNEKNLYLGMDYLLNKSKVN
                     DLKGDTLIIDEYGEVEDLAYNLKSKKEVKDSSWNMSINKTTKVFLMISFITIIVV"
     gene            complement(2062..>3282)
                     /locus_tag="KRL67_003738"
     CDS             complement(2062..>3282)
                     /locus_tag="KRL67_003738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003362983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HBH1524570.1"
                     /translation="FSLFSIWSLLLINIILAMGGYIFYFKNFDKEIKEIDEYPMISIL
                     VPAHNEAKVIGRTVESLLLLNYPKSKMELIVINDNSSDNSKEILENIKDRYNNYNFTI
                     INTDSLTGGKGKSNALNIGYTISKGDFIAVYDADNTPDKNALRYLVQTIVMNDELGAV
                     IGKFRTRNKNKNLLTKFINIETLSFQWMSQAGRWQLFNLCTIPGTNFILRRSIIEEIG
                     GWDSKAIAEDTEISFRIYKLGYKIKLVPQSITWEQEPETVKVWIKQRTRWAKGNIYVL
                     MKYIKNIFKQGRNKIVFDIAYFFSVYFLFLTSVIISDILFALSISKLVEISIPINFFL
                     IWILSYLLFIIEVSISLTIEKGEATIENIFIVAIMYFTYSQLWLFVAIKGMIEYLKDI
                     IFKREVKWYKTERF"
BASE COUNT         1094 a          522 c          312 g         1354 t
ORIGIN      
        1 atgactacaa ttatagtaat aaaagaaatc attaaaaata ctttagttgt cttatttata
       61 ctcatattcc agcttgaatc ttttacttct ttcttagatt ttaaattata agctaaatct
      121 tctacctctc cgtattcatc aattatcaat gtatctcctt ttaaatcatt aactttactt
      181 ttattcaaca aataatccat tcctaaatat aagttttttt cattcattga actaattacc
      241 atgatatttt tttgattatt atatggtgat tttattaatt gaatggtaga aatatttttt
      301 ccataatcat caataaagct tatcttatca tttgatataa aatcggagta atttttatcg
      361 aactttatat ttaaattatt atttaacatc tttataacag aattattatg tggtactcca
      421 aaaactacaa tattagtatc cttatacttg tcactaaatt cttttcctct aattacattt
      481 atatttccat tatgtgaatt tatattcgca cctaaagtaa cacctaatct aaacatccag
      541 gtcatagctt gtgaccctga ataatctggc attatcacag ttaaatcatt aaattcatca
      601 tctctaacaa atggatacgg ataattttca aaagataagg tttcattttc tttcgtagat
      661 aacgccaaat aagaattatt tgatacatat gcccaaggat tattactctc tctagtaaca
      721 caattcgaat tttttatact caaattaaat gtcaatttaa cttgataata gtttttatta
      781 tctatatctt taggtatttt taattctaac ttatcattat tagaatgtga cctatccaat
      841 tttttacttc ccacaataac atcatttata gacactgtaa ctaaagattt ttcaaaatct
      901 aaattgtcag aatatcttaa atttaagata atctttgaac catcatctaa caccttaccc
      961 gttggtattt tgacatcaaa caaagcttgt tgattaaatg ctccttccaa caaaaagtca
     1021 gaatatccta agtcttttaa tgttaaatga cctaaattta atttttgttc cctattgatt
     1081 tttatattag tctctttgtt tacaaatact gatgatgata aaacttgatt agaaagtcta
     1141 ttttctatta ataaatttga tgcctttatt aaatcatctt cattactacc aattaccacc
     1201 atcatcttct tattcttatt gtatggtgaa tctacttgct tgattataca gtttgaagat
     1261 aatagggttt gttcctttgt acttaaaata tccaaaattt cttctgctgt attttcaggt
     1321 ttaccaatat atataatatt atcatctgac caatttttca tttctgaata taatttaaca
     1381 tctaacttta aatcatcatt ttttgttatt tttccaaatg ctgatgccaa attaaacact
     1441 gctgtagtct ctcctctagt catattatct ggtactacta tagttgtatc taatttatga
     1501 ttattttcaa tttctgcata tggatacgga tattcattta ttgaatttcc catctttttc
     1561 tgtttatatc taattgatat atatgattct ttgtgaataa ccatccagtt cccagtattg
     1621 gaatcatctt ggcaaatttt atcagatatt gttttgtatg tttttatttt tatttcgtta
     1681 tatccttgaa ttacataatc ttttggaact aatacttcta gtgtattttt ataatttgtc
     1741 ttagcattta atttaataga ttttattggt acattattta tcaaaacagt taaacttgaa
     1801 acatctccat ttaatatttg actcttacta aaattaagat gtaataaaac ttcttcaata
     1861 tcccaatttt tatttacttc aaaaaaagta ctattacttc ctataacacc atcaatagtt
     1921 atatctcttt caaacttata atttttaact ttagtctcgt cagcatttac tttgtatata
     1981 agtgatatat ttgaaaaaaa caatactaaa gatataattg atataataaa ttttttcatg
     2041 ttaattcctc ctgtacatga atcaaaatct ttctgtttta taccatttaa cctctctttt
     2101 aaaaattata tcttttagat actctatcat tccttttata gctacaaaca gccatagttg
     2161 agagtatgta aaatacatta ttgcaactat gaatatattt tcaattgttg cttcaccttt
     2221 ttctatagta aggcttatac tcacttctat aataaataaa agataagata gtatccatat
     2281 tagaaaaaag ttaattggaa tagaaatttc tactagttta gatatactta aagcaaataa
     2341 aatatcggat ataatgacag atgttaaaaa tagaaaatat acagaaaaaa agtaagctat
     2401 atcaaataca atcttatttc ttccctgttt aaatatattt tttatatatt tcattaatac
     2461 atatatattc ccttttgccc atctagtacg ttgctttatc cacaccttaa cagtttctgg
     2521 ctcttgttcc catgttatag attgtggtac aagctttatc ttgtatccca atttatatat
     2581 cctaaaactt atttctgtat cttctgcaat tgctttacta tcccatcctc caatttcttc
     2641 tatgatactt ctcctcaata taaagttagt tcctggtatt gtacatagat taaaaagttg
     2701 ccatctacca gcttgtgaca tccattgaaa gcttagtgtt tctatgttta taaatttggt
     2761 taataggttt ttatttttat ttcgagtcct aaatttccca ataacagcac ctaattcatc
     2821 attcattact atagtttgta ctaaatatct aagtgcattt ttatctggtg tgttatctgc
     2881 atcataaaca gctataaaat ctccttttga aatcgtatat ccaatattta atgcgttaga
     2941 ttttcctttt ccacccgtta aactatctgt atttattata gtgaaattat aattgttgta
     3001 cctatccttt atattttcta aaatttcttt actattatca gaagaattgt catttataac
     3061 aataagttcc atcttactct taggataatt gagtaatagc aaagactcca cagttctacc
     3121 tatcacttta gcttcattat gagcaggaac aagtattgat atcataggat attcatcaat
     3181 ttcttttatt tctttatcaa agtttttaaa ataaaatata tatccaccca tagctaaaat
     3241 aatatttata agcaataaag accatatact aaacaaacta aa
//
LOCUS       DAEPXF010000284         3278 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain HB27
            SAMN13376389-rid12479423.denovo.292, whole genome shotgun sequence.
ACCESSION   DAEPXF010000284 DAEPXF010000000
VERSION     DAEPXF010000284.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13376389
            Sequence Read Archive: SRR10518432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3278)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3278)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001069624.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001069624.1
            Genome Coverage       :: 315x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/15/2021 01:54:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,165
            CDSs (total)                      :: 4,076
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 89
            rRNAs                             :: 10, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 10 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 19 of 69
            Pseudo Genes (incomplete)         :: 44 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 10 of 69
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3278
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13376389-rid12479423.denovo.292"
                     /strain="HB27"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="China: Hebei"
                     /collection_date="2017"
     gene            complement(56..628)
                     /locus_tag="KRL67_003739"
     CDS             complement(56..628)
                     /locus_tag="KRL67_003739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000265278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese catalase family protein"
                     /protein_id="HBH1524571.1"
                     /translation="MWIYQKTIQHPVNIKTCDPRMAKFLITQFGGPNGELAASLRYLS
                     QRYTMPTGNMRALLTDIGTEELAHVELICTMVYQLTSDASPEELKAAGLGSNYAQNGY
                     GIYPTDSNGVPFDVRPIAVMSNPVTDLHEDMAAEQKALATYYQLINLTDDVDVIDVLK
                     FLGQREIIHYQRFGEALMDAYELEESQKMF"
     gene            complement(657..923)
                     /locus_tag="KRL67_003740"
     CDS             complement(657..923)
                     /locus_tag="KRL67_003740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore coat protein CotJB"
                     /protein_id="HBH1524572.1"
                     /translation="MKDSSRAELMRKIQETSFACVDMNLYLDNHPDDKNAINTYNSLC
                     NQFAQARYAYENKYGPLTNFGYAPSRCPWQWADQPWPWDREFNY"
     gene            complement(923..1132)
                     /locus_tag="KRL67_003741"
     CDS             complement(923..1132)
                     /locus_tag="KRL67_003741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore coat associated protein CotJA"
                     /protein_id="HBH1524573.1"
                     /translation="MENKTRDIKKNCGPSLARPYINNQIFTEMYCLSDALKFGTIFPE
                     LNLLESEMYNKELYAKPKKYRGGRR"
     gene            1401..2261
                     /locus_tag="KRL67_003742"
     CDS             1401..2261
                     /locus_tag="KRL67_003742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011986089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine(32) synthetase TtcA"
                     /protein_id="HBH1524574.1"
                     /translation="MSELSGKGCEIIVPFEERLPVRDIEKSIIKKYRKNLWSKFMKAI
                     RDYKLVEEGDKIAVAISGGKDSILMAKMFQELKKHGQVNFDVEFIAMDPGYHANIRQL
                     LIDNCEYLNIPIHLFDSRIFEIADEIAKDYPCYMCARMRRGALYSKAEELGCNKLALG
                     HHYDDVIETTMLNLLCAGNFKTMLPKLNSTNFEGIKIIRPLYYIREEHIIRFIQNSGI
                     WPLNCACMVAAKKTGNKRYEIKDLIKSLEPNFKNVEKSIFKAAENVNLDSVLGWQKDG
                     EKHSFLENFE"
     gene            2309..>3278
                     /locus_tag="KRL67_003743"
     CDS             2309..>3278
                     /locus_tag="KRL67_003743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease domain-containing protein"
                     /protein_id="HBH1524575.1"
                     /translation="MKRVYIDFEMNMPNSKSKRAVFNSDIIAIGAVMYDEKTKNIDKF
                     KSLIRPVSNEDLYPHIQELTHISSEELESAPSYEEVMRKFKKWLGIFSDIKGIYTFGN
                     LDLTCFNNTDMRSAKKNNHPRFVNNIRDLFVDIKEKYINCGMRCMNYISLKNLLEFVN
                     LEFSGDAHDPLNDAYNLFILDEAITNNVDIQNLLIIRDIIRPPFNDINSNLDNCFNKF
                     KESLYKKEGNYNIVDFSVEIIKTVRMYLLTIIDVNIQNLEIMKDISKKMDTIDKLKDI
                     EEGYFYLLEDVYFDMKDVLEDLMLYRMNEDEYKYEIKNIIKMFDEDL"
BASE COUNT         1256 a          381 c          538 g         1103 t
ORIGIN      
        1 aatacaacag taaattataa attatcaaat aataatagtt atccaaataa aaattttaga
       61 acattttttg agattcttct aactcgtaag cgtccattaa agcttcacca aatctttgat
      121 agtgaattat ttctctttga cctaagaatt ttaatacatc tataacatca acatcatctg
      181 ttaggtttat aagttgataa taagttgcaa gtgctttttg ttcagctgcc atatcctcat
      241 gtaaatcggt tacaggattt gacataactg ctataggtct tacatcaaat ggaacaccat
      301 ttgaatctgt tggataaatt ccatatccat tttgagcata gtttgaacca agacctgcag
      361 cttttaactc ttctgggctt gcatcagaag tcaactgata aaccatagta catataagct
      421 caacgtgagc tagttcttct gtaccaatat ctgttagaag tgcacgcata ttacctgtag
      481 gcattgtgta tctttggctt aaatatctca atgatgcagc aagttctcca tttggtccac
      541 caaattgagt tataagaaat ttagccattc tagggtcaca agtttttata ttaactgggt
      601 gttgtatagt tttctgatat atccacatga attttcaatc ctccaatact tgatgtctaa
      661 taattaaatt ctctgtccca aggccaaggt tggtcagccc attgccaagg gcatctactt
      721 ggagcatacc caaagttagt taaaggaccg tatttatttt cgtaagcata tctagcttgc
      781 gcaaattgat tacataatga attataagtg ttgatagcat ttttatcatc gggatgattg
      841 tctaaatata agttcatatc aacacatgca aaagatgttt cctgaatttt tctcataagt
      901 tcagctctag atgaatcttt cattatcttc tacctcctct atattttttt ggcttagcat
      961 ataattcttt gttgtacatt tcagattcta gtaagtttag ttctggaaat atagttccaa
     1021 attttaatgc atcagaaagg cagtacattt ctgtgaaaat ttgattatta atgtatggtc
     1081 tagccaaaga tggaccacaa ttttttttga tatctctagt cttattttcc aagatgaaaa
     1141 cctccttgtt gtaaatagat aggtaactct atctagtgta taatattcaa tcatttaaaa
     1201 atcgttacta ataataaaat attaaatgag aaaaataaaa aagataataa aagaaagagg
     1261 ttatttatgt tattataagt ttatgtgtta ttgtaaacta taaattattt agtaaaatta
     1321 aagtaaaaat tatagtattt tgtgtatgaa ggtagacttt tttgtaaaat attattaaca
     1381 taaatatacg aggtgattag atgagtgaac tatcaggaaa aggctgtgaa attatagttc
     1441 cttttgagga aagacttcca gtaagagata tagaaaaaag tataataaaa aaatatagaa
     1501 aaaatttatg gtctaagttt atgaaggcaa taagagatta taaattagtt gaagaagggg
     1561 ataagatagc tgtagcgatt tctggtggaa aagacagcat tcttatggct aaaatgtttc
     1621 aagagttaaa aaaacatgga caagttaatt ttgatgttga atttattgct atggaccctg
     1681 gataccatgc aaatataaga caattactta tagataattg tgagtatcta aatataccaa
     1741 tacatttatt tgattcaaga atatttgaga tagcagatga aatagcaaaa gattatccat
     1801 gttatatgtg tgcaagaatg aggagaggag cattatattc aaaagcagaa gaattagggt
     1861 gtaacaaact tgcattaggt catcattatg atgatgtaat agagacaaca atgcttaact
     1921 tattgtgtgc tggaaatttt aaaacaatgc ttcctaaact taattctact aattttgaag
     1981 gtataaaaat tataagacca ctttattata taagagaaga acatattata agatttattc
     2041 aaaatagtgg tatatggcca ttaaattgtg catgtatggt tgccgctaaa aaaacaggta
     2101 ataaaagata tgagataaaa gacttaataa agagcttaga accaaatttt aaaaatgtag
     2161 aaaagtctat atttaaggcg gcagaaaatg taaatcttga ctcagttctt ggctggcaaa
     2221 aagatgggga gaaacattct ttcttagaga attttgaata aaaatagtta aaaagaatca
     2281 ctataaacaa ttggaaaggg tgtttatatt gaaaagagta tatatagatt ttgaaatgaa
     2341 tatgccaaat tctaaatcaa aaagagcagt atttaattct gatataattg caatcggagc
     2401 agttatgtat gatgaaaaaa caaaaaatat tgataaattt aaatctttaa taagaccagt
     2461 aagtaatgag gatttgtatc ctcatataca agaattaaca catatatctt ctgaagaatt
     2521 ggagagtgca ccaagttatg aagaggtcat gagaaagttt aaaaaatggc ttggcatatt
     2581 ttctgatatt aaaggaatat atacttttgg taatttggat ttgacatgtt tcaataacac
     2641 agatatgaga agtgctaaaa aaaataacca tccaaggttt gtaaataata ttagagattt
     2701 atttgtagat ataaaagaaa aatatataaa ttgtggtatg agatgtatga actatatatc
     2761 attgaaaaat ttacttgaat ttgttaactt agagttcagt ggagatgccc atgacccatt
     2821 aaatgatgca tataatttat ttattttaga tgaggcaatc acaaataatg tagatataca
     2881 aaatctttta ataatacgtg acataataag accaccattt aacgatataa attctaattt
     2941 agataattgt tttaacaaat ttaaggagtc attatataaa aaagaaggaa attataatat
     3001 tgtagatttt tctgttgaaa taataaaaac tgtgagaatg tacttattaa caatcataga
     3061 tgtaaatata caaaatttag agattatgaa agatataagt aaaaaaatgg atactataga
     3121 taaattgaaa gatatagagg aaggctattt ttatttatta gaagatgtgt acttcgacat
     3181 gaaagatgta ctagaagatt taatgcttta tagaatgaat gaagatgagt acaagtatga
     3241 gataaaaaat ataataaaaa tgtttgatga agacttag
//
LOCUS       DAEPXF010000285         3278 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain HB27
            SAMN13376389-rid12479423.denovo.293, whole genome shotgun sequence.
ACCESSION   DAEPXF010000285 DAEPXF010000000
VERSION     DAEPXF010000285.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13376389
            Sequence Read Archive: SRR10518432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3278)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3278)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001069624.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001069624.1
            Genome Coverage       :: 315x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/15/2021 01:54:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,165
            CDSs (total)                      :: 4,076
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 89
            rRNAs                             :: 10, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 10 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 19 of 69
            Pseudo Genes (incomplete)         :: 44 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 10 of 69
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3278
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13376389-rid12479423.denovo.293"
                     /strain="HB27"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="China: Hebei"
                     /collection_date="2017"
     gene            76..687
                     /locus_tag="KRL67_003744"
     CDS             76..687
                     /locus_tag="KRL67_003744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HBH1524576.1"
                     /translation="MINDIKNKFKDYKPYINGYKNMKRASVLIPIVEINNTHYILFEV
                     RSKNLKHQPSEISFPGGKIESGETPYEAVIRETCEELGTFSDNIEVISSLDLLITPVK
                     FIIHPYLGYIKNINNLNINKDEVDHTFLVPIKYLLENPPNTYSNTVKVFPDENLPYGS
                     IPNQKDYKFESGSYPILFYKYKDYVIWGMTAKILENFLNFLSE"
     gene            985..1323
                     /locus_tag="KRL67_003745"
     CDS             985..1323
                     /locus_tag="KRL67_003745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3784 domain-containing protein"
                     /protein_id="HBH1524577.1"
                     /translation="MFAVGFVVIIALAFFILSVLLFNGKASMLLAGYNSMSKKEKARY
                     DKKKLCRSSSIVTFIVSIMLFIMAFLGYRIESGQMDEKKMVPFSIIFIIVILSSVFFN
                     ILYSNKKCKK"
     gene            complement(1730..3166)
                     /gene="miaB"
                     /locus_tag="KRL67_003746"
     CDS             complement(1730..3166)
                     /gene="miaB"
                     /locus_tag="KRL67_003746"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HBH1524578.1"
                     /translation="MSKRKQVTIPIEKIQEQDKYIEQIHRQNEEYFNRTGKRKLVFTQ
                     TFGCQMNEHDSEKLCSMLEEMGYQMSMMVEESDLIIYNTCAVRENAELKVYGNLGQLK
                     HLKGKNPDMKIAVCGCMMQQPHVVEELRKKYKHVDLIFGTHNLYKFPQLLTESINSDK
                     MLVDVWDVDGEVIEGLRSNRKFELKAFVNIMYGCNNFCTYCIVPYTRGRERSRTPEDI
                     INEIKELVANGTKEITLLGQNVDSYGKTLENSVSFSELLRKVNDIEGIERVRFMTSHP
                     KDISDEVIYAIRDCDKVCEFLHLPIQCGSSSLLKKMNRHYTKEYYLEIIEKAKKEVPG
                     IAFSTDLMIGFPGETEEDLLDTLDVVEKVRYDSAFTFIYSKRQGTPAAKMENQIPEDI
                     KHDRFNRVLEAVNRISAEINDGYKDKIVEVLVEGRSKNNENKFAGRTRQNKLVNFEGG
                     NDDLIGKLVMVKITEPRTFSLNGILVNN"
BASE COUNT         1131 a          476 c          375 g         1296 t
ORIGIN      
        1 agataaatca taattatata ttattgttaa aactcaatta tataattttt aataatatta
       61 ttttaggagg taaaaatgat aaacgatatt aaaaataaat tcaaagatta caaaccatat
      121 ataaatggat acaagaatat gaagagagca tctgttttaa ttccaatagt agaaataaat
      181 aacacacact atatattatt tgaagttcgc tcaaaaaatt tgaagcatca acctagcgaa
      241 atttcttttc caggtggtaa aatagaatca ggtgagactc catatgaagc tgttattaga
      301 gaaacttgtg aggaacttgg aacttttagt gacaatatag aagtaatatc aagtcttgac
      361 ctccttataa caccagtaaa gtttatcata catccatact taggatatat aaaaaatatt
      421 aataatttaa atataaacaa ggatgaagtt gaccatactt ttttggttcc tataaaatat
      481 cttttagaga atcctccaaa tacttatagt aatactgtaa aagtttttcc agatgaaaat
      541 ctaccatatg gttcaattcc aaaccaaaaa gattataaat ttgaaagtgg aagttatcct
      601 attctgtttt ataaatataa agattatgta atttggggaa tgacagcaaa aatactagaa
      661 aactttttaa attttttatc agaataacac tcataataaa tcattaaaac ttttacataa
      721 gaaatttata aaagtttcta aatttaacaa ttctaaaata tcaggaacta tattttgtag
      781 cacttttttg ttttattatt gtttgaatat ttgatggttt tcaatgcaat ttcaacataa
      841 atactatgta ttagtgtata ctgttgatga attaaagcat gataatctat ttaataaaat
      901 atgaagtaaa tgattttaat gtgtctttat ggtaaaatta ctaataagta ataaaattaa
      961 ttaaaatata tggaggtgct tataatgttt gctgtaggat ttgttgttat aattgcactt
     1021 gcctttttca ttttaagtgt actattattt aatggaaaag ccagtatgct actagcaggt
     1081 tataactcta tgagtaagaa agaaaaagca cgatacgata aaaaaaaatt atgtagaagt
     1141 tctagcattg ttacttttat tgtctctata atgctattta ttatggcatt tttgggatat
     1201 agaatagaga gtggtcaaat ggacgaaaaa aaaatggttc ccttttctat aatatttata
     1261 atagtcatac tttccagtgt attttttaat atcctctact ctaacaaaaa atgtaaaaaa
     1321 taaatagttg taagttgagc agttaattat aaaagattga ctgctttttc atgtctattg
     1381 actactctta ttaaatattt taacatttaa aaatctatgt aaaaataatg aatatagtac
     1441 aaatttctaa taatgatttt acctttgtgg ttacagtcca ctatatataa tttaatatga
     1501 taacagggaa ttactaatga caactaagta agcttttata aaatataata aaacaaaaat
     1561 agatgtacaa tgaaattata tcttatatca aatttatttt tattaatagc agaaacttac
     1621 attagcataa cataagacaa attcgcttaa aataaaaatt tttaatataa tttgttctat
     1681 agtacatcta ttttgattag tttacataat tttattattt tgtacaatat taattattta
     1741 ctagtatccc atttaatgaa aaagttctag gttcagtaat tttgaccata actaatttac
     1801 caattaaatc atcatttcca ccttcgaaat ttacgagttt attttgtcta gttctaccag
     1861 caaacttatt ttcattattt ttacttcgtc cttcaactag aacttcaact attttatctt
     1921 tatatccatc atttatttct gcagaaattc tatttacagc ttctaacacc ctattaaatc
     1981 tatcatgctt tatatcttct ggtatttgat tttccatttt agcagcagga gttccctgtc
     2041 ttttagaata aataaatgta aaagctgaat catatctaac tttttcaacc acatctaaag
     2101 tgtccaataa atcttcttct gtttctcctg gaaaaccaat cattaagtct gtagaaaaag
     2161 caatacctgg aacttccttt ttagcttttt ctattatttc caaataatat tcctttgtat
     2221 aatgtctatt catttttttc aaaaggctac tgcttccaca ttgtattggt agatgtaaaa
     2281 actcacacac cttatcacaa tctctaattg cgtatattac ttcatcagat atatcttttg
     2341 gatgagatgt cataaatcgt accctttcaa ttccttctat atcattcact tttcttaaaa
     2401 gttctgagaa tgaaactgaa ttttcaagtg tcttaccata agaatctaca ttttgtccaa
     2461 gtaaagtgat ttctttcgtt ccatttgcaa caagctcttt tatctcgtta attatatctt
     2521 caggagttct acttctttct cttcctcttg tgtatgggac tatacaatat gtgcagaaat
     2581 tattgcatcc atacattata tttacaaatg cttttaattc aaattttcta ttacttctaa
     2641 gaccttcgat tacttctcca tccacatccc atacatctac taacattttg tcagaattta
     2701 ttgattctgt aagtaattgc ggaaatttat atagattatg tgttccaaat ataaggtcta
     2761 catgtttata tttttttctt aattcctcta caacatgtgg ttgttgcatc atacatccac
     2821 atacagctat tttcatatct ggattttttc cttttagatg ttttaattgt cctaagttac
     2881 catacacttt taactctgca ttttctctaa ctgcacatgt gttatatatt attaaatcac
     2941 tttcttcaac catcattgac atttggtatc ccatctcttc taacatcgaa cataattttt
     3001 ctgaatcatg ttcattcatt tggcacccaa aggtttgagt aaacacgagt tttctctttc
     3061 ctgttctgtt gaagtattct tcattttgtc tgtgtatttg ttctatatat ttatcttgtt
     3121 cttgtatttt ctctataggt attgttactt gttttctttt gctcacgtct attcctccta
     3181 gttaatttct tatattacac ataaccttaa ttatatcaaa taagaaaaat cttttaaaga
     3241 tattattatt tttctagttg tattttaata gtaaatct
//
LOCUS       DAEPXF010000286         3073 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain HB27
            SAMN13376389-rid12479423.denovo.294, whole genome shotgun sequence.
ACCESSION   DAEPXF010000286 DAEPXF010000000
VERSION     DAEPXF010000286.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13376389
            Sequence Read Archive: SRR10518432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3073)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3073)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001069624.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001069624.1
            Genome Coverage       :: 315x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/15/2021 01:54:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,165
            CDSs (total)                      :: 4,076
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 89
            rRNAs                             :: 10, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 10 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 19 of 69
            Pseudo Genes (incomplete)         :: 44 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 10 of 69
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3073
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13376389-rid12479423.denovo.294"
                     /strain="HB27"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="China: Hebei"
                     /collection_date="2017"
     gene            82..1524
                     /locus_tag="KRL67_003747"
     CDS             82..1524
                     /locus_tag="KRL67_003747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding repeat-containing protein"
                     /protein_id="HBH1524579.1"
                     /translation="MRTIRRVLALGLTLAIFLINVPNVDALTSDTIKGNNIYETAGLI
                     ADKKSYDTAIMVNMDNSIADGLSASGLAGAVDAPILLAQKNKIPNETKQRLKNVKKIY
                     IIGKELSISKSVETELKNTGAQVTRLGGDDRIKTSYSVAKEVSGIKKVDEVILTNAYK
                     GEADTISAAPVSVRDIAPIVLTDGKSVPFSTSNVKTYAVGGSISMSTSLVNKTNAKRL
                     GGSDRYDTNKKVIKEFYPDASEFYLSDGYDLVNALTGSTIAKENPIVLVSESSDKSIL
                     AGADKITRLGSISDSVYNKCVSAAQNNGDSSTKGESPMKNETSILGQPTASLEACLKW
                     AKSKKANDLFIELIPILYDTAVQEGVNPVLAVAQSAKETGFCNFGGVLDASFKNPCGL
                     KTSVGGSDTDKNAHSRFDTWEEGILAQIQHLCLYAGQDGYPLSNPVDPRHEKSLFGKA
                     KTVESLSNNWAGGQYGQDLVRMMGEIEATK"
     gene            1566..1919
                     /locus_tag="KRL67_003748"
     CDS             1566..1919
                     /locus_tag="KRL67_003748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH1524580.1"
                     /translation="MKKSSIFSIIFPTVIMVLMFLTFATDIFNIPDIQSKSIFVVGMV
                     LIFPISFLIQGIICVLNKTNWILSLIVSLVTYIVLMYIFLNDSAYIYVLLYTAFYMIG
                     HITAKLYYKIKTFKN"
     gene            2110..2931
                     /locus_tag="KRL67_003749"
     CDS             2110..2931
                     /locus_tag="KRL67_003749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphatase HisJ family protein"
                     /protein_id="HBH1524581.1"
                     /translation="MNLIDNHIHTNFSSDGKDSMEDTIKKAISIGVRYLTFTDHLEHD
                     EDRGFCINYNDYVPVFNKFKEKYKKDIELLLGVEVGYRKHLKNEIEKIINSNPFDFVL
                     CSTHTIDNVPVPSKAYFKGLSKEDAYYKYFNSILETNREFGDYNIYGHLDYISRYGIY
                     SDNRVIYNDFKDVIDEVLKSIINNGSGIELNTSGYRYGLNAIHPNEDILKRYKELGGF
                     IVTVGSDSHRVEDICKDFDVAYDMLKYLDFKYVSLFKERETYFVNIEKVKSNNIA"
BASE COUNT         1185 a          316 c          546 g         1026 t
ORIGIN      
        1 ataataaatg actatagcgt agatgtacta ctatgttata gtcatttttg aattaaactt
       61 gatatatcaa gggggaagaa tatgagaaca attagaagag ttttagcatt aggtttaaca
      121 ttagctatat ttctaataaa tgtacctaat gtagatgcat taacatcaga tacaattaaa
      181 ggtaacaata tatatgagac ggctggatta atagcagata aaaagagtta tgatacagca
      241 attatggtga atatggataa ttctatagca gatggccttt cagcaagtgg tcttgcaggt
      301 gctgttgatg ctccaatttt gcttgcacaa aaaaataaga taccaaatga aacaaaacaa
      361 agattaaaaa atgttaaaaa gatatacata ataggtaaag aattatcaat aagtaagtca
      421 gtagaaactg aactgaaaaa cacaggggca caggtaacta gattgggtgg agatgacaga
      481 ataaagacta gttatagtgt tgctaaagag gtaagtggta tcaaaaaagt tgatgaagtg
      541 atattaacta atgcatataa aggagaagca gatacaataa gtgctgcacc tgtatcagta
      601 agagatatag ctcctatagt acttacagat ggaaagagtg tgcctttttc aacaagtaat
      661 gtaaaaacat atgcagtagg tggaagtata tccatgagca ctagtttagt taataaaact
      721 aatgctaaaa gacttggtgg ttcagataga tatgatacaa ataagaaagt tataaaagag
      781 ttttatccag atgcatcaga attttattta agtgatggat atgatttagt aaatgcactt
      841 acaggttcta caattgctaa ggaaaatcca attgtattag tgtcagaaag tagtgataag
      901 tctatattag caggagcaga taagattact agattaggtt caataagtga cagtgtgtat
      961 aataagtgtg tttctgctgc acaaaataat ggcgattcgt ctacaaaagg tgagtcacct
     1021 atgaaaaatg agacaagcat attaggtcag ccaacagcta gtttagaagc gtgcttaaaa
     1081 tgggccaaat ctaaaaaagc taatgattta tttatagagt taataccaat attatatgat
     1141 actgctgttc aggaaggtgt taatcctgtt ttggcagtag ctcaatctgc aaaagaaact
     1201 ggtttttgta attttggtgg agtattagat gcatcattta agaatccttg tggacttaaa
     1261 acttctgtag gtggttctga tactgataag aatgcccatt caagatttga tacttgggaa
     1321 gaaggaatat tagctcaaat tcaacattta tgcttatatg caggacaaga tggttaccca
     1381 ctttcaaatc cagtagaccc tagacatgaa aaatctttat ttggtaaggc taaaacagtt
     1441 gaaagtctat ctaataattg ggctggaggt caatatggac aagatttagt aagaatgatg
     1501 ggagaaattg aggcgacaaa gtaattgaag attatcaaat atccaataaa taaggattga
     1561 ttattatgaa aaagagtagt atttttagta tcatatttcc tacagtcata atggtgctaa
     1621 tgtttttaac ttttgctaca gatatattta atattccaga tattcaatct aaaagtattt
     1681 ttgttgtggg aatggttttg atttttccaa tttctttttt gatacaagga ataatttgtg
     1741 tattaaataa gactaattgg attttatctc ttattgtgtc tttagtaact tatatagtat
     1801 taatgtatat atttttaaat gactctgcat atatatatgt tttactttat acagcttttt
     1861 atatgattgg acatataaca gctaaactct attacaaaat taagactttt aaaaattaaa
     1921 ttgaagtata taaaaataat attaagagaa gtatctttaa tgattttcta aaatcaaaaa
     1981 taggtgcttc tcttttttgt aaaaataaaa gtatgtaaat aaaatttaac cattataatt
     2041 ttcttttaac aaatgtatac aataaacttg ttgtataatt aagatagata aaataaaggg
     2101 agggataata tgaatttaat agataatcat attcatacta atttttcaag tgatggaaag
     2161 gattccatgg aagatacaat aaaaaaagct ataagtattg gtgttagata tttaactttt
     2221 acagaccatt tagaacatga tgaagataga ggattttgca taaattataa tgactacgtt
     2281 ccagtattta ataagtttaa agaaaaatat aaaaaggaca tagagttatt actaggtgtt
     2341 gaagtgggtt atagaaaaca tctaaaaaat gaaatagaaa aaataataaa ttctaatcct
     2401 tttgattttg tattgtgttc aactcataca attgacaatg tacctgttcc atcaaaggcg
     2461 tattttaaag gacttagcaa agaagatgca tattataaat attttaatag catacttgaa
     2521 actaatcgtg aatttggaga ttacaatata tatggtcatt tggattatat atctaggtat
     2581 ggaatatatt cagataatag agttatatac aatgacttta aagatgttat tgatgaggtc
     2641 ttaaagtcaa taatcaataa tgggagtggt atagagctaa atacttctgg ttatagatat
     2701 ggactaaatg ctatacatcc aaatgaagat attctaaaaa gatataaaga acttggagga
     2761 tttatagtta ctgtaggttc tgattctcat agggtagaag atatatgtaa ggattttgat
     2821 gtggcctatg atatgcttaa gtatcttgac tttaaatatg tatctttatt taaagaaaga
     2881 gaaacttatt ttgtaaatat agaaaaagtt aaatctaaca atatagctta gagtgtgttt
     2941 tgaaataaat taatggatag ttaaaaagtt aatagttata atattgtatc ctctagaaaa
     3001 atactagagg tttttatttt gctaaaataa ctaaatagaa ttaaatttaa atagtatagt
     3061 ttattttaaa ttg
//
LOCUS       DAEPXF010000287         3067 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain HB27
            SAMN13376389-rid12479423.denovo.295, whole genome shotgun sequence.
ACCESSION   DAEPXF010000287 DAEPXF010000000
VERSION     DAEPXF010000287.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13376389
            Sequence Read Archive: SRR10518432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3067)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3067)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001069624.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001069624.1
            Genome Coverage       :: 315x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/15/2021 01:54:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,165
            CDSs (total)                      :: 4,076
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 89
            rRNAs                             :: 10, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 10 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 19 of 69
            Pseudo Genes (incomplete)         :: 44 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 10 of 69
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3067
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13376389-rid12479423.denovo.295"
                     /strain="HB27"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="China: Hebei"
                     /collection_date="2017"
     gene            complement(<1..426)
                     /locus_tag="KRL67_003750"
     CDS             complement(<1..426)
                     /locus_tag="KRL67_003750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBH1524582.1"
                     /translation="MSGIISVMTQSLILSIMALGVYITYKILDFPDMSADGSYTMGAS
                     IVAFSLTNGISPVVATLMAILCGCIAGLVTGILHIKFKISNLLSGILVMGMLYSINLR
                     IMGKSNIPLFSFKHLFNGEISPIVLALAFVFICKVLLDLF"
     gene            complement(1235..2272)
                     /locus_tag="KRL67_003751"
     CDS             complement(1235..2272)
                     /locus_tag="KRL67_003751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBH1524583.1"
                     /translation="MIKSKKILSLIIAGVLGVSMLTGCSQNDGSNASNENKKTDSKKQ
                     KNIGITQLVEHPSLDKAKKGFIKALEDKGYKDGDNIKIDFQNAQNDMPTTQSIASKFV
                     SDKKDLIYAISTPSAQAAYNATKDIPIIMTAVTDPVEAGLVKSLEKPGGNVSGTSDYL
                     SIDKTLELVKTLTPKAKKIGVIYNTSEVNSKIQVDSLHDYAKKNNYEVVEKGISSSSE
                     VNQAISSLVGKIDVLYVPTDNLIVSSMPIVSKVANENKIPIIASEEGSVSSGALACCG
                     IDYEKLGYKAGELAIEVLEGKSVGDIPVTTLDETEIIINEDTLKALDMQKLSADNIKY
                     IKSDENAKSAK"
     gene            complement(2369..>3067)
                     /locus_tag="KRL67_003752"
     CDS             complement(2369..>3067)
                     /locus_tag="KRL67_003752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003399531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBH1524584.1"
                     /translation="TIFENLSIDIEDGEFVSIIGSNGTGKSTLLNIISGLIKESSGQI
                     TLDSTTITNLAEYKRTQIVSRVFQDPSLGTCPSMTVRENLSLALNKGKLLNLKKCLRH
                     KTNYLEHLLEGISLDLKKYLDIKVQYLSGGQRQSLSLIMSSLASPKVLLLDEHTAALD
                     PKTSNEVIGLTDKIVREKNITTLMVTHNLKHALQYGDRLIMLHKGEVVLDVKDKEKED
                     LTVEEILEKFEYAV"
BASE COUNT         1051 a          473 c          397 g         1146 t
ORIGIN      
        1 gaataggtct aaaaggactt tacagatgaa tacaaaagct aaggctaaaa ctataggaga
       61 tatttcccca ttgaataagt gtttaaagct aaatagagga atatttgatt ttcccattat
      121 tcttaaattg atagagtata gcattcccat aactaaaata ccagaaagta aattagatat
      181 cttaaactta atgtgaagaa tccctgtaac aagaccagca atacaaccac ataagatagc
      241 cattaaagtt gctactacag gagaaatacc attagtaaga gaaaatgcta cgatagaagc
      301 acccattgtg tagctaccat cagcagacat atctgggaaa tctaagattt tgtaagttat
      361 ataaaccccc agtgccataa ttgataaaat taaactttgc gtcattacag atataatacc
      421 tgacataaat aaaacactcc tttgaaaata tttttataat tttaattatt tgctaatttt
      481 gtgtataaaa aaaccacata gtgtacaaaa ctatgcggta aaaacacgaa taagccacat
      541 agtttaatct atgtggctct tatatagtta aatgaaaact ttataaacaa tatagccaac
      601 atagatacaa tcccaaaatt ttagtagtct taaggggtct gtatctatgt taaaacgtag
      661 gtacaaaccc taaaaatagt ggctatagta ataataatat tttttattgt ttaataaagt
      721 attcataatt atctctccct tttaatattt taattagtca gaaaattata tgtattctcg
      781 acttgttata aattataaat tatacaataa taaaagtcaa ctgctaaatt taattaaaat
      841 actaaataat gtatactcag aatattttta aattaatgta ttatttgcaa aaaacgttat
      901 cctaagttat aagtgaatgt ggaaatttta tcgaaatatt tactaaatat ggaataacta
      961 gacacatata tcaatcaaat ctcataaaaa aaagctgtct agagaatatt aaaattcttt
     1021 tggacagctc aagtagtgat tagaaagtta tataactttc ataatcagtc gtcaatatag
     1081 acgactttta tggaaaatga ataactttat atgcaatatt caaattgcta caataagcac
     1141 aattttcaaa gggttagggt ggataagagt acttattata taaagttttc atattaccta
     1201 cttcatatta cctatttttc gaaattaata agttttattt tgcagatttt gcattttcat
     1261 ctgactttat atactttata ttatctgctg ataacttttg catgtcaagt gcttttagtg
     1321 tatcttcgtt aattattatt tcagtttcat ctaatgtagt aactggtata tcgccaacag
     1381 attttccttc taatacttca atagcaagtt ctccagcctt ataacctagt ttttcataat
     1441 ctattccaca acaagctaaa gcaccagaag atacagaacc ttcttcagag gctattatag
     1501 gaatcttgtt ttcattagca actttagaaa ctattggcat agaagaaact attaaattgt
     1561 cagtagggac atataaaaca tctattttgc caactaaact agaaatagct tggttaactt
     1621 cacttgaaga gctgattcct ttttcaacta cttcataatt atttttctta gcataatcat
     1681 gtagagaatc aacttggatt tttgaattaa cttcactagt attgtatata acccctattt
     1741 tctttgcttt tggagtcaat gttttaacca attctaatgt tttatcaatt gaaagatagt
     1801 cagatgtacc agaaacattt ccacctggtt tttcaagaga tttaacaagt ccagcttcta
     1861 cagggtctgt aacagcagtc attattatcg gaatatcttt agtagcatta taagctgcct
     1921 gtgcagatgg agtagatata gcatatatca aatctttttt gtcggataca aacttactag
     1981 caatactttg tgtagtaggc atatcatttt gtgcattttg gaaatctatt tttatattat
     2041 ctccatcttt atagccttta tcttcgagtg ctttgatgaa tcctttcttt gctttatcca
     2101 gagatggatg ttccactaat tgagtaatac ctatattttt ttgttttttg ctatctgttt
     2161 ttttattttc atttgaagca ttggagccat cattttgaga acatccagta agcattgata
     2221 caccaagtac tcctgctata attaaactta atattttttt actttttatc attttatttt
     2281 cctccctcaa aatttaataa ttatatttgt tcatatattc atataataga ttataaatat
     2341 ttctgattaa aactaagatt ttaatttcct atactgcata ttcaaatttt tctaagattt
     2401 cttcaactgt taaatcttct ttttctttat cttttacatc caaaacaact tcacccttat
     2461 gaagcattat caacctatct ccatactgaa gagcgtgttt caagttgtga gtaaccataa
     2521 gtgtagttat atttttttct cttacgattt tgtctgttaa ccctattact tcatttgatg
     2581 tttttgggtc aagtgctgct gtatgttcgt ctagtaataa gaccttagga cttgctaaac
     2641 tagacataat tagtgatagt gattgtcttt gacctcctga aagatattgt actttaatat
     2701 ctaaatattt ttttaaatcc aaagaaattc cttcaagtaa gtgttccaaa taattcgttt
     2761 tatgacgaag acattttttt aagttcaata atttcccttt attaagtgct agagataaat
     2821 tttctctaac cgtcatagaa ggacaagttc caaggcttgg gtcttgaaaa actctactta
     2881 ctatttgagt ccttttatac tcagctaaat ttgttatggt agtactatct aaagtaattt
     2941 gaccagaaga ttcttttata agcccagata ttatatttaa taaagtagat ttaccagttc
     3001 cattactacc tatgatactt acaaattcac catcttctat atcgattgat aaattttcaa
     3061 atatagt
//
LOCUS       DAEPXF010000288         3048 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain HB27
            SAMN13376389-rid12479423.denovo.296, whole genome shotgun sequence.
ACCESSION   DAEPXF010000288 DAEPXF010000000
VERSION     DAEPXF010000288.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13376389
            Sequence Read Archive: SRR10518432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3048)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3048)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001069624.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001069624.1
            Genome Coverage       :: 315x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/15/2021 01:54:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,165
            CDSs (total)                      :: 4,076
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 89
            rRNAs                             :: 10, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 10 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 19 of 69
            Pseudo Genes (incomplete)         :: 44 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 10 of 69
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3048
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13376389-rid12479423.denovo.296"
                     /strain="HB27"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="China: Hebei"
                     /collection_date="2017"
     gene            99..605
                     /locus_tag="KRL67_003753"
     CDS             99..605
                     /locus_tag="KRL67_003753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HBH1524585.1"
                     /translation="MKEYVVVDLETTGLDPYKGCEIIEIGVTEIKNEQIVKNYSRLIK
                     PKGIISSFITELTHISNEMVENEEPLELVLPRFRKYIGDRTIIAHNAKFDLKFLNYYL
                     RMLDLEPINNYICTVELLKKCKSYKGKNKKLETACAYYNIENINAHRADSDTLATAKL
                     FLKIKDEY"
     gene            733..1749
                     /locus_tag="KRL67_003754"
     CDS             733..1749
                     /locus_tag="KRL67_003754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HBH1524586.1"
                     /translation="MGYYNDNDNNKIRRKKISNSKDSNKKSKHSYTSANKKNKSSNTS
                     SSKRMSEKVDSQNLIKRDRDSFTRDNVRPLNTDYNQRERRRVEYKQREKKLKSKKRKK
                     QKRIRIVRALLSIMLVALIIFSVAGAIFTVSAIKGSPEVTKKLIRSKYISSEVVSIKQ
                     MPNDLKHAIVSIEDERFYKHKGVDVISLVRSVLNNVFTDTTQGASTIDMQVSKNLLTS
                     NDKTMKRKIRDMYNAKQMNKIMTKDEILEAYLNNMYLGKDAYGAAKGAEVYFGKKVSE
                     LNLAECAMLAGMTNNPARYIDHGEAKKRQVIVLYKMHQLGYITDKEYRIAKADDTPFK
                     SEID"
     gene            2103..2807
                     /locus_tag="KRL67_003755"
     CDS             2103..2807
                     /locus_tag="KRL67_003755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="B3/4 domain-containing protein"
                     /protein_id="HBH1524587.1"
                     /translation="MKKFVIEDAFWNLFPSAKIGVVICNDIDNSIKDEDYKKIILKGE
                     EEALKYLEDSEFSNNQVIKVWREAFKKFKTKKGARSSIEALLKRVHNGNNIGTINPLV
                     DIYNFISLKYALPCGGEDIDKFIGDIRLTRAIGNEEFIPLGTDENSFPYEGEIIYKDD
                     GGAICRCWNWREAVRTMLTEDTNNAFLCIELVDESRFKEFENALNELAKIVQEKLGGK
                     CKISILDIDNKEVSVD"
BASE COUNT         1292 a          273 c          536 g          947 t
ORIGIN      
        1 tgtaaaatct tcctatatca tataaaatta agtattgatt agtctattac ataaatatag
       61 aatataaata tatccattta ataataagga gaagagatat gaaagagtat gtagttgtgg
      121 atttagaaac tacaggatta gatccatata aaggttgtga aataatagaa ataggagtaa
      181 cagaaataaa aaatgaacaa attgtaaaaa actattcaag gttaataaaa cctaagggga
      241 taatttcttc atttataaca gaattgacac atataagtaa tgaaatggta gaaaatgaag
      301 aaccactaga attagtttta cctagattta gaaaatacat aggtgataga actataattg
      361 cacacaatgc gaaatttgat ttgaaatttt taaattacta tcttagaatg ttagatttag
      421 aaccaataaa caattatata tgtacagtag agttattaaa aaaatgtaaa agttataaag
      481 gaaaaaataa gaaacttgaa acagcttgtg cttattacaa catagagaat ataaatgctc
      541 atagagctga cagtgataca ctagcaactg caaaactatt tttgaaaata aaagatgaat
      601 attaattata aatatagaga aaattagtga aaatatcctt tataaaattg acccttaaac
      661 ttacaatttt gttctttgat ttattttgta gaacaatgta taatgtacct tatatgaaga
      721 agggagacta aaatgggtta ttacaatgat aatgataata ataaaataag acgtaaaaaa
      781 attagtaata gcaaagatag taataaaaaa tctaaacatt cttatacttc ggcaaataaa
      841 aaaaataaaa gttctaatac aagttctagt aaaagaatga gtgaaaaagt agattcacag
      901 aatcttataa aaagagatag agatagtttt acaagagata atgtaagacc tttaaataca
      961 gattacaatc aaagagaaag aagaagagtc gagtataaac aaagagagaa aaaattaaaa
     1021 agtaaaaaaa gaaaaaaaca aaaaaggatt agaatagtta gggcattgtt atctattatg
     1081 ttagttgcac taattatatt ttcagtggca ggtgcaatat ttacagtttc agctataaaa
     1141 gggtcaccag aagttactaa aaagctaatt aggtctaaat atataagtag tgaggttgta
     1201 agtataaaac aaatgccaaa tgatttaaaa catgcaatag tttctataga agatgagaga
     1261 ttttataagc ataaaggagt agatgttatt tctctagtta ggtcagtatt aaacaatgtg
     1321 tttacagata caacacaagg tgcaagtact atagatatgc aagtttcaaa aaacctgctt
     1381 actagtaatg ataaaactat gaagcggaaa ataagggata tgtataatgc taagcagatg
     1441 aataagatta tgactaagga tgaaattctt gaagcatatt taaataatat gtatctaggt
     1501 aaagatgcat atggagctgc aaagggagct gaagtctact ttggtaaaaa agttagtgaa
     1561 ttaaatttag ctgagtgtgc tatgttagct ggtatgacaa ataatcctgc aaggtatatt
     1621 gaccatggag aggctaagaa gagacaagtg attgtattgt ataagatgca tcagcttggt
     1681 tatataactg ataaggagta tagaatagca aaagctgatg acactccatt taaatcagaa
     1741 attgattaaa attgtaagaa tatagtattt tcaatagttt aaacaaatga aaaggtaata
     1801 aaaaaggtga tttgccaccc atttgccatc ataattataa tctaatggca aattgtttta
     1861 atgtacaaat cagataatat aatattgttt taaatactag attatgctaa aatttaattg
     1921 tattttgtaa attaaaaaat ttataactta gttttctagg gttccgcatt ttataatggt
     1981 caggtccaag agaaaacgca caattattgt gtacacggaa ggataaaagc ctgggagata
     2041 tttaataata tttctatagg cttttttatt tttacatatt ttaaatttga ggaggttttc
     2101 taatgaaaaa atttgttatt gaggatgcat tttggaatct atttcctagt gcaaaaattg
     2161 gagttgttat ttgtaatgat atagataact ctataaaaga tgaagactat aagaaaataa
     2221 ttttgaaggg agaagaagaa gcattaaaat acttggagga ctcagaattt agtaacaacc
     2281 aagtgattaa agtatggaga gaagcattta aaaaatttaa aacgaaaaaa ggagcaaggt
     2341 cttctattga ggcattatta aaaagagttc ataatggaaa taatatagga actattaatc
     2401 ctttagtgga tatctacaat tttatctcat taaaatatgc attaccttgt ggaggggagg
     2461 atatagataa atttatagga gatattaggc taactagagc aattggtaat gaggagttta
     2521 tcccactagg tacagatgaa aattcatttc catatgaagg agaaataatt tataaagatg
     2581 atggtggagc aatttgtaga tgttggaatt ggcgtgaagc agtgagaaca atgcttactg
     2641 aagatactaa caatgccttt ttatgtattg aattagtaga tgaaagtaga tttaaagaat
     2701 ttgaaaatgc tttaaatgaa ttagctaaaa tagttcaaga aaaattgggt gggaagtgta
     2761 aaatttcaat tttagatatt gataataaag aagtatcagt agattaatct ggaggtgctg
     2821 ttgtaataga ttattttagt caaaaagata cttatattga ggataggaaa atttatttta
     2881 gatataacaa ttattaaagt atataatgag gattagtatt ctattgataa gtgataagat
     2941 aaaaaatatt gtcaactaat tatctttaga taaaataatt gttaaaagaa caaattatga
     3001 aaagtttaag aaaaaacttg gtatacctaa tgaaagattt gaagaaat
//
LOCUS       DAEPXF010000289         2992 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain HB27
            SAMN13376389-rid12479423.denovo.297, whole genome shotgun sequence.
ACCESSION   DAEPXF010000289 DAEPXF010000000
VERSION     DAEPXF010000289.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13376389
            Sequence Read Archive: SRR10518432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2992)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2992)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001069624.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001069624.1
            Genome Coverage       :: 315x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/15/2021 01:54:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,165
            CDSs (total)                      :: 4,076
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 89
            rRNAs                             :: 10, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 10 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 19 of 69
            Pseudo Genes (incomplete)         :: 44 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 10 of 69
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2992
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13376389-rid12479423.denovo.297"
                     /strain="HB27"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="China: Hebei"
                     /collection_date="2017"
     gene            389..1666
                     /gene="hflX"
                     /locus_tag="KRL67_003756"
     CDS             389..1666
                     /gene="hflX"
                     /locus_tag="KRL67_003756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HBH1524588.1"
                     /translation="MDMAKKGITVGLNINNKSEDFNELMIELENLCSACDIDVVGSIT
                     QNAKQVNRAFYIGTGKVEELLNLIKKENIEIVIFYNELSTSQLKNLEEKLNCEIIDRT
                     ALILDIFAQRAKTREAKLQVEVASLKYMLPRLIGSNENLGRQSGGVGTKNRGSGEKKL
                     ELDRRRIEEKITSLNKELDDLKFQRETQRSMRRKSNLPNVALVGYTNAGKSSIMNKLV
                     DIFKNSEEKKVFEKNMLFATLETSVRNIVLTNNKEFLLSDTVGFVSNLPHDLVKAFRS
                     TLEEACEADVLLHVIDISNPSYKSHIKVTEDTLKQIGADGIPMIHVYNKIDLIDVEVL
                     DKILDSIDKEGLFVSVKKDINIDKMIKCICDSIFKDYVRCKFLIPYDKGHVASYFNEN
                     TSVINTEYREDGALLDVECSNIEYNKYKKYVLE"
     gene            1908..2405
                     /locus_tag="KRL67_003757"
     CDS             1908..2405
                     /locus_tag="KRL67_003757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HBH1524589.1"
                     /translation="MDAETLMKKFMEDDDSRKQAIFASIFVMQNKLQTTCDKLDPELT
                     MKQWLLLAIADSVDETLTLTKLGELMGCSRQNVKKLAITLEKKGFIQIEQNEKDSRAI
                     CLVTKDRVEEYSKKVRDMQEKVLKLLFEDFTEKEVEQLFFGIMKLSTGIENVKNYVEN
                     SDVGE"
     gene            2454..2978
                     /locus_tag="KRL67_003758"
     CDS             2454..2978
                     /locus_tag="KRL67_003758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HBH1524590.1"
                     /translation="MKSVVVIYKSKYGSTEKYAKWLSELLSCDIFETKNINIEKVMKY
                     DTIVLGGGLYACNIAGISFFKKNYKELQKKNLVVFGVGAAPYDDEVIKKLREVNFKDE
                     LNKVPCFYCRGAWDETKMSFIDYTLCSFFKKSIAKKERQGLKPVEAPLLSGEAFDWCN
                     KENLTPILDYIREL"
BASE COUNT         1236 a          270 c          532 g          954 t
ORIGIN      
        1 ttacttttat taatcatgca aataactact taaatttaag aatagggtta aatataaaat
       61 gataattaca tgtaagttaa ttaaactaat gtatttttaa tatatattta gaactttaat
      121 ccttcttatg atataagtag cttgttttgt attacttaga tttgtaagga gtttttttgt
      181 tgaaaaactg cataatatat ttgggaggag gtgtaagaaa gtgatttttt atataagatt
      241 gagaaaataa tgcacgatta tggttattat aatggtaaat aagaagtgtc atttaaggat
      301 aagaaaaaag taaaattctt gatgaattta attatagagc cataatctcg tgctatagat
      361 taaataaaaa atagcagata aggagaatat ggatatggcg aaaaaaggaa taacagtagg
      421 attaaacata aataataaat ctgaggactt taatgaacta atgattgaac ttgaaaactt
      481 atgttcagca tgtgatattg atgttgtagg gagtataact caaaatgcaa agcaagttaa
      541 tagagcattt tacattggga caggtaaagt tgaggaacta ttaaacttga taaagaaaga
      601 gaacatagag atagttatat tttacaatga actttctaca tcacaactaa aaaatcttga
      661 agaaaagctt aattgtgaaa ttatagatag aacagcattg atacttgata tatttgcaca
      721 aagggcaaaa actagagaag ctaaacttca agtagaagta gcaagtttaa aatatatgtt
      781 accaagactt ataggttcaa atgaaaattt aggacgtcaa agtggtggag ttggtactaa
      841 aaatagaggt tctggtgaga aaaaattaga acttgaccgt agaagaatag aagaaaagat
      901 aacatcttta aataaggagt tagatgattt aaaattccaa agagaaactc aaagaagtat
      961 gagaagaaaa tcaaatcttc caaatgtagc tctagttggg tatacaaatg ctggtaaatc
     1021 ttcaataatg aataaactgg tagatatatt taaaaactct gaagaaaaga aagtgtttga
     1081 gaaaaacatg ttatttgcta cacttgaaac ttctgtcaga aatatagttc ttacaaataa
     1141 caaagaattt ttattatctg atactgttgg ttttgtaagt aatctaccac atgacttagt
     1201 aaaagcattt aggtcaacat tagaagaagc atgtgaggca gatgtactgt tacatgttat
     1261 tgatatttca aatccaagct ataaaagtca tattaaagtg acagaagata ctttaaaaca
     1321 aataggtgca gatggtatac ctatgataca tgtgtataat aaaattgatt taatagatgt
     1381 agaagtatta gataaaattt tagatagtat cgataaagaa ggcctatttg tttcagttaa
     1441 aaaagatata aatatagata aaatgataaa atgtatttgt gatagcatat ttaaagatta
     1501 tgtaagatgt aagttcttaa ttccatatga caaaggtcat gtagcttctt actttaatga
     1561 gaatacgagc gttataaata cagaatacag agaagatgga gctcttttag atgtagaatg
     1621 tagcaatata gaatacaata aatataaaaa gtatgtttta gagtaagttt tgaaaaaatt
     1681 attatagctt aaaatacatt taatttataa ttttataata aattataaaa ttattgtaaa
     1741 gtatgatttt atgaaacggt atagccataa agctatgccg ttttgccatt tataataaaa
     1801 ataaatttat tgacacaatg gtaactaatt actataatat aaagtaacta gttactattt
     1861 aaagttatca aaaaattaaa atctaagata aagagaagtg tataataatg gatgcagaaa
     1921 ctttaatgaa aaaatttatg gaagatgatg atagtagaaa acaagctata tttgcaagta
     1981 tatttgtaat gcaaaataaa cttcaaacta catgtgataa attagaccca gaattaacaa
     2041 tgaaacaatg gttactttta gctattgcgg actctgtaga tgaaactctt acattaacca
     2101 aacttggaga attaatgggg tgctcaagac aaaatgtaaa aaaactagct attactttag
     2161 aaaagaaagg ttttatacaa attgagcaaa atgaaaaaga ttcaagagct atctgtcttg
     2221 tgacgaaaga tagagtggaa gagtattcta aaaaagtaag agatatgcag gaaaaagtat
     2281 taaagctttt atttgaagat tttacagaga aagaagtgga acagttattt tttggaatta
     2341 tgaaattaag tacaggaatt gaaaacgtga aaaattatgt agaaaatagt gacgttggag
     2401 aataagcaag tgtcaaatca actttgtatg acatcagaaa gggagtaatt tatatgaaaa
     2461 gtgtagtagt gatttataaa agtaagtatg gttcaacaga aaaatatgct aaatggcttt
     2521 cagaattatt gtcttgtgat atttttgaaa ctaagaatat taatattgaa aaggttatga
     2581 agtatgatac gattgtttta ggaggaggtc tttatgcttg taatatagca gggatttctt
     2641 tctttaaaaa aaattataaa gaattacaga aaaaaaatct agtagtattt ggtgtaggag
     2701 ctgctccata tgatgatgaa gtaataaaga agttaagaga agtaaacttt aaggatgaat
     2761 taaacaaagt tccttgtttt tattgtcgtg gggcatggga tgagacaaaa atgtctttta
     2821 tagattatac tctttgtagt ttttttaaaa aatctatagc taaaaaagaa cgtcaaggtc
     2881 ttaaaccagt agaagcacct ttattgtctg gagaagcctt tgattggtgt aataaagaaa
     2941 atttaactcc aatacttgat tatataagag aactttaaga ggtatatatt tg
//
LOCUS       DAEPXF010000290         2984 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain HB27
            SAMN13376389-rid12479423.denovo.298, whole genome shotgun sequence.
ACCESSION   DAEPXF010000290 DAEPXF010000000
VERSION     DAEPXF010000290.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13376389
            Sequence Read Archive: SRR10518432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2984)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2984)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001069624.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001069624.1
            Genome Coverage       :: 315x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/15/2021 01:54:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,165
            CDSs (total)                      :: 4,076
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 89
            rRNAs                             :: 10, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 10 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 19 of 69
            Pseudo Genes (incomplete)         :: 44 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 10 of 69
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2984
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13376389-rid12479423.denovo.298"
                     /strain="HB27"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="China: Hebei"
                     /collection_date="2017"
     gene            139..507
                     /locus_tag="KRL67_003759"
     CDS             139..507
                     /locus_tag="KRL67_003759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HBH1524591.1"
                     /translation="MENLVKIFKALSDETRLNILILVSKRNICQKGISKYLGISDSAV
                     SQHIKILKDVGIITGYKEGYYVLYHINKESFNICVDFINSMLSNSSESFIDVFDVNTI
                     HLGCSKECKSIKKCCKRREK"
     gene            510..881
                     /locus_tag="KRL67_003760"
     CDS             510..881
                     /locus_tag="KRL67_003760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifB/NifX family molybdenum-iron cluster-binding
                     protein"
                     /protein_id="HBH1524592.1"
                     /translation="MKVCIPVEENKGLDSKPYGHFGSAPIFVVCDLESGEVKSLDNGD
                     LDHEHGKCQPLKALSGTAVDAVVVGGIGQGAIIKLNGMGIKVYRAEGDTISANLDLLK
                     NGKLVEFPSDHTCSHDGCGHH"
     gene            complement(1017..1472)
                     /locus_tag="KRL67_003761"
     CDS             complement(1017..1472)
                     /locus_tag="KRL67_003761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH1524593.1"
                     /translation="MNINTVQGDSIEVLLRQLGATRISKVSSTLYFIKFDLGDGWEIS
                     YTYNINAKDQYFLQRIEPYPIGRGLFNDEYEIVSFISKDLKKFLNAKNSSNFKTFVEV
                     TRKVNSIIDNVEELFLNYNVDGDDLKTLNKELNDILNDIDYIEKHTKKI"
     gene            complement(1511..2776)
                     /locus_tag="KRL67_003762"
     CDS             complement(1511..2776)
                     /locus_tag="KRL67_003762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsB/NhaD family transporter"
                     /protein_id="HBH1524594.1"
                     /translation="MEFQQIIAICIFLIVMAAIITEKVNRSVAAVGGALLMIIFNILT
                     LDEGLSHIDFNTIGVLVGMMLFVAVVKNSGLFEYIAIWTAKKAKGDPWKIMICFAIIT
                     AILSAVLDNVTTVLLIGPMTIVITQILGLNPVPFLITQILASNIGGTATLIGDPPNIM
                     IGSAANLSFMDFVINLGPAVIVILAITIICFRFIYGKELVVNERAKNAILKLDEKKSV
                     KDKPLLIKSLILIAFILFGFMFHSTIHIDSSVVALTGASIMLLIGKQDVDEIMAGIEW
                     STILFFMGLFVVVGGLVEVGIINKLAQALIGLTEGHLVFTMLLILWLSAIVSSFLDNI
                     PFVATLIPLILTMQAEGIDVMPLWWATSLGACLGGNGTLIGASANVVLAGIGNKHGHP
                     ISFKEYFKIGFPLMIISIIISTVYLIIKF"
BASE COUNT         1112 a          414 c          411 g         1047 t
ORIGIN      
        1 ttaaaaaaat tcaaagttat attaggaaaa attgggatta tttataatat accccaaaat
       61 ttaaaaataa tggttgaaaa atctaattaa gagtactata atttaattaa gtaattactt
      121 aatcaaaacg aggtacttat ggaaaatttg gttaaaatat ttaaagcatt gagtgatgaa
      181 actagactta acatacttat attagtatcc aagagaaata tatgtcaaaa ggggatttct
      241 aaatatctag ggatttcaga ttctgctgtt tcacaacata taaaaatatt aaaagatgtt
      301 gggataataa caggatataa agaaggatac tatgttttat atcatattaa taaagaatca
      361 tttaatatat gtgtagattt tataaattct atgttaagta attctagtga aagctttata
      421 gatgtatttg atgtgaatac aatacatcta ggatgcagta aagaatgtaa atctataaaa
      481 aaatgttgta agagaaggga gaaataataa tgaaagtatg tataccagta gaagagaata
      541 aaggattgga tagcaaacca tatggacatt ttggttctgc tccaattttt gttgtgtgtg
      601 atttagaaag tggagaagtt aaatctttag ataatggaga tttagatcat gaacatggaa
      661 aatgtcaacc attaaaagca ttgtcaggga cagctgtaga tgcagtagtt gttggaggaa
      721 taggacaagg agctataatt aaattaaatg gaatgggaat aaaagtttac agagcagaag
      781 gtgatactat aagtgctaat ttagacctac ttaaaaatgg aaaattagta gagtttccat
      841 cagaccatac ttgctcacat gatggatgtg gacatcatta aaaaaaacag ctgatgaaat
      901 atatgcttca tcagctattt tttcttttaa taaatacaat tttattcttt taaaaccaaa
      961 atttagattt ttatgttttt tgtagtatcc ttgataatat cccaagttta ctacctttag
     1021 attttttttg tatgtttttc tatatagtca atatcattta atatgtcatt aagttcctta
     1081 ttaagtgttt ttaaatcatc accatctaca ttgtagttta aaaatagttc ctctacatta
     1141 tcaatgatag aattgacttt tctagtaact tcaacgaatg ttttaaagtt actactattt
     1201 ttagcattta gaaatttttt taaatccttt gatataaatg atactatttc atattcatca
     1261 ttaaatagac ctctaccaat aggatatggt tcaattctct gtaaaaaata ttggtcttta
     1321 gcattaatat tataagtata agatatttcc caaccgtctc ctaaatcaaa cttgataaaa
     1381 tataatgtac tacttacttt tgatattctt gtggcaccaa gttgtcttaa aagaacttca
     1441 atcgaatcac cttgaacagt attaatattc atatatacac atccccttta gtattattat
     1501 actatatttt ttaaaatttt ataattaaat aaacagtaga tataataata gatataatca
     1561 taagtggaaa acctattttg aaatactctt taaaagagat aggatgaccg tgtttattac
     1621 ctatacctgc tagtacaaca ttagcagatg ctcctattag tgttccattt cctccaagac
     1681 aagctccaag agatgttgcc caccatagtg gcattacatc aataccttca gcttgcatag
     1741 tcaaaataag tgggattagt gttgcaacaa atggaatatt atctaagaaa gaagagacta
     1801 tagctgatag ccataatatc aataacattg taaatacaag atgtccttct gtaagcccaa
     1861 tcaatgcctg agccaatttg tttattatcc caacttctac taatccacca actactacaa
     1921 ataatcccat aaagaataaa attgtagacc attcaatacc tgccataatt tcatctacat
     1981 cctgttttcc tattaaaagc attatagatg ctcctgtcag tgctacaaca gaagaatcta
     2041 tgtggattgt actgtgaaac ataaaaccaa aaagtatgaa tgctattaat ataagacttt
     2101 ttataagtag aggtttatct ttaacagatt ttttttcatc taattttaat attgcatttt
     2161 tagctctttc attcacaaca agttcttttc cataaataaa tctaaagcat attatagtta
     2221 tagcaagtat tactataaca gcaggaccta aatttattac aaaatccata aaactaagat
     2281 tggcagcact accaatcata atatttggag ggtctcctat aagagtagct gttcctccta
     2341 tatttgaagc taaaatctgt gttattaaaa atggaactgg attaagtcct aatatttgtg
     2401 taataactat agtcattggt ccaataagta agacagttgt aacattatct aatacagctg
     2461 ataaaatagc tgttataata gcaaagcaaa tcattatctt ccatgggtca cctttagctt
     2521 tctttgcagt ccagatagct atatattcaa ataaccccga gtttttgaca acagctacaa
     2581 atagcatcat accaactaat acaccaatag tgttgaaatc tatatggctt aatccttcat
     2641 caagtgtaag tatattgaat attatcatta atagtgcacc accaacagct gctacggatc
     2701 tgtttacctt ttctgtaata atagctgcca taactattaa aaatatacat atagctatta
     2761 tttgttgaaa ttccataata taagtacctc catttgtatt attttgtgcc taatgctagt
     2821 actatatata gtattacttt tttattgttt tttcaatgga tgtatgaaag tatattattt
     2881 aacaaagttt ttgaaaaatt gacagaattt aaagaaaatt tttgttattt aaataaattt
     2941 taaaattatt ccattgataa taatattaaa cgacaaacaa aaaa
//
LOCUS       DAEPXF010000291         2961 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain HB27
            SAMN13376389-rid12479423.denovo.299, whole genome shotgun sequence.
ACCESSION   DAEPXF010000291 DAEPXF010000000
VERSION     DAEPXF010000291.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13376389
            Sequence Read Archive: SRR10518432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2961)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2961)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001069624.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001069624.1
            Genome Coverage       :: 315x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/15/2021 01:54:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,165
            CDSs (total)                      :: 4,076
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 89
            rRNAs                             :: 10, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 10 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 19 of 69
            Pseudo Genes (incomplete)         :: 44 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 10 of 69
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2961
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13376389-rid12479423.denovo.299"
                     /strain="HB27"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="China: Hebei"
                     /collection_date="2017"
     gene            90..884
                     /locus_tag="KRL67_003763"
     CDS             90..884
                     /locus_tag="KRL67_003763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBH1524595.1"
                     /translation="MIEVDKLCKSFTRVVKDDKNKSSIRKLKKVKTKKEEFFAVNNVS
                     FKVMEGEIVGILGPNGAGKTTLLRMLGGILTPTSGSINISGYDYSIDRNSAKKEIGYL
                     SGNTKLYGRLSPRELLTTFASLYEMSKEEIEESIGNVVNIMDMSEFIDNRIENLSTGQ
                     TQRTSIARCLIHSPKVYIFDEPTLGLDVLSSASIIDFMKNEKLRGKTVLYSTHYMEEA
                     ETLCDKIFMIHNGKIIASGTPQSLKEESGVNNLRDVFIKLARKEEI"
     gene            885..2945
                     /locus_tag="KRL67_003764"
     CDS             885..2945
                     /locus_tag="KRL67_003764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HBH1524596.1"
                     /translation="MRSKIVKYIFKKEMLDILRDKKTLFMMIVVPLLLYPLLMLLMIQ
                     VMNMSASSMNSKDINIAFNNKPNKVLVSMIEDDNLEKDTKNTSNDKGKIRVLDVDNYK
                     KSLEENKIDAYIKMEEKKSSTNYQIYINSSQENSSNAVKRIETVLDKYKRFTIEKTLS
                     EKGLDVQATLEPITYSTVDVAKTEEVAGYFLGQILPFILIIGVLLGSIYPAIDVMAGE
                     KERGTLETLFTLPISNLELVMGKYMAVSLSAIVTAILNILSMGLTMAFILVSGGISSQ
                     FGFGNFNYGDLVLPIITTLICICLFSMVVSAISMCVCSLAKSFKDAQNYITPLMLIIM
                     IPSYVSMIPNIVLDRVTAVIPVVNISLLIKSVLSFKSDLNLIALVLASNVAFVILAII
                     LLSKMFNSEEILFGNSKSFSFLEKRSNIKKGSIPTVSDGVILYAVGLVLLIYVGSLVQ
                     IKFGMTGIIMTQFMIISLPLLFAYYIKADFKEVFSLKVPKLKHVFGSIVLWIGGYILI
                     MIITQLILFLFPQNMEVAESLNKALFIKDSVLLNLLVIALLPAICEEMFFRGFIFSSF
                     SKSKDKNKSIKLAIICSGVLFGIMHMDFVRIIPTSILGIIFAYSVYKSGSIFVSMLLH
                     FLNNSVAVVLNHYTTGNITNLYKFVEVDFSNLNVLKFALILLISLILVMLGLRVLDRK
                     SLRN"
BASE COUNT         1123 a          306 c          500 g         1032 t
ORIGIN      
        1 ttctaaaaca aaaaactgtg tataaaaaag gtttatatgt tatatttata taaatataac
       61 atatgaatga gaaaggagta gtttgataaa tgattgaggt agataaatta tgcaagtctt
      121 ttacaagggt cgtaaaagat gataagaata aaagttcaat aagaaagtta aagaaagtaa
      181 aaactaaaaa agaagaattt tttgctgtaa ataatgtttc atttaaagtt atggaaggtg
      241 aaattgttgg aatattaggt ccaaatgggg caggaaagac tacattactt agaatgttag
      301 gtggcatatt aacaccaaca tcaggaagta taaatatttc tggatatgat tattcaatag
      361 atagaaattc agcaaaaaaa gaaatagggt atttgtctgg aaatacaaaa ttatatggaa
      421 ggctttctcc tagagaactt ctaacaactt ttgcaagtct atatgaaatg tcaaaagagg
      481 agatagaaga atcgattgga aatgttgtaa acataatgga tatgagtgaa tttatagata
      541 atagaattga aaatctatca actggtcaaa ctcaaagaac atctatagct cgttgtttaa
      601 tacattctcc taaagtatac atttttgatg agccaacact aggtctggat gttttaagta
      661 gtgcatcaat tatagatttt atgaagaatg aaaaactaag gggaaaaact gttctttact
      721 caacccatta tatggaagaa gcagaaactt tatgtgacaa gatttttatg attcacaatg
      781 ggaaaataat tgcatcagga acaccacaga gtttaaaaga ggaatccgga gtaaataatt
      841 tgagagatgt attcataaag cttgccagaa aggaggagat ttaaatgaga agtaagattg
      901 taaaatacat atttaaaaaa gaaatgttag acatattgag agataaaaaa actttattta
      961 tgatgattgt tgtgccactt cttttatatc ctttacttat gttattaatg attcaagtaa
     1021 tgaatatgtc tgcaagtagt atgaatagca aggacattaa tatagctttt aataataaac
     1081 ccaataaggt gttagtatcc atgattgaag atgataattt agaaaaagat actaaaaata
     1141 cttcaaatga taaaggcaaa ataagagtgt tagatgtaga taactataaa aagtcattag
     1201 aagaaaataa aattgatgct tatataaaga tggaagaaaa aaaatcatct acaaattatc
     1261 aaatatatat aaattcttct caagaaaatt cttctaatgc agtaaaaaga attgaaactg
     1321 ttttggataa atataaaaga tttaccatag aaaaaacttt atctgaaaaa ggattagatg
     1381 tacaagctac tttagaacca ataacatata gcactgtaga tgttgcaaag acggaagaag
     1441 tggcaggata ctttttaggt cagatacttc catttatatt aataatagga gttttactag
     1501 gttcaatata tccagcaata gatgttatgg ctggagaaaa ggaaagaggt acattagaaa
     1561 cattatttac actcccaata tcaaacttag aactggttat gggaaaatat atggcagttt
     1621 ctcttagtgc aatagtaaca gcaattttaa acattttatc tatgggactg actatggcat
     1681 ttattttagt tagtggaggt attagtagcc aatttggctt tggaaacttt aattatggag
     1741 acttagtctt accaattata actactttaa tttgtatatg tcttttttca atggtagttt
     1801 cagcaataag tatgtgtgta tgttcattag caaaaagttt taaagatgct caaaactaca
     1861 taacacctct tatgttaatt attatgatac catcatatgt ttctatgata ccaaatatag
     1921 tattagatag agtaacagct gtaatacctg tagtaaatat atctttactt ataaaaagtg
     1981 tattatcttt taaaagcgat ttaaatttaa tagccttagt tttagcatca aatgtagcct
     2041 ttgttatatt ggcaatcata ctactgtcta agatgtttaa ttctgaggaa atactttttg
     2101 gtaatagtaa gagtttctct ttcttagaga aaagaagtaa tataaaaaaa gggtctatac
     2161 caactgtaag tgatggtgtt attttatatg cagtaggatt agttctattg atttatgttg
     2221 gttctttagt tcaaattaaa tttggtatga caggtattat aatgacacaa ttcatgataa
     2281 tttctttgcc gttattattt gcatactata ttaaagctga ttttaaagag gtatttagct
     2341 tgaaagttcc taaattaaag cacgtgtttg ggagcattgt tctttggatt ggtggatata
     2401 tacttattat gattataaca cagctaattt tattcttatt tcctcaaaat atggaagttg
     2461 ctgaaagttt aaataaggct ctttttatca aagatagtgt tttattgaat ttactagtaa
     2521 tagctttatt accagctata tgtgaagaaa tgttttttag gggatttata ttttcttcat
     2581 ttagtaaatc aaaagataaa aataagtcta tcaagcttgc tataatatgt agtggggtac
     2641 tgtttggtat tatgcatatg gattttgtta gaataattcc aacatcaata ttaggtatta
     2701 tatttgcata cagtgtgtat aaatctggtt caatatttgt atcaatgtta ttgcattttt
     2761 taaataatag tgtagcagtt gtgttaaatc attatacaac tggaaatata accaacttat
     2821 ataaatttgt tgaagttgat ttttcgaact taaatgtact taaatttgct ttaattcttc
     2881 taataagtct tatattagtt atgttaggat taagagtttt agatagaaaa tctttaagaa
     2941 attaagtata aagttattat a
//
LOCUS       DAEPXF010000292         2959 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain HB27
            SAMN13376389-rid12479423.denovo.300, whole genome shotgun sequence.
ACCESSION   DAEPXF010000292 DAEPXF010000000
VERSION     DAEPXF010000292.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13376389
            Sequence Read Archive: SRR10518432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2959)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2959)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001069624.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001069624.1
            Genome Coverage       :: 315x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/15/2021 01:54:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,165
            CDSs (total)                      :: 4,076
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 89
            rRNAs                             :: 10, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 10 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 19 of 69
            Pseudo Genes (incomplete)         :: 44 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 10 of 69
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2959
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13376389-rid12479423.denovo.300"
                     /strain="HB27"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="China: Hebei"
                     /collection_date="2017"
     gene            57..467
                     /locus_tag="KRL67_003765"
     CDS             57..467
                     /locus_tag="KRL67_003765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HBH1524597.1"
                     /translation="MNKKKILVPIDGTERSMHSLEFIKGIFKKDEVEVEIMNVKELVF
                     IDGISLAEEIKNSENLGRRILDKAAEIMGEYDVKVHFTFGYPGDEIIRKAKEDNVDFI
                     VMTKSTKKGLTRMIGSVTASVVKQAKCIVMIVPE"
     gene            489..791
                     /locus_tag="KRL67_003766"
     CDS             489..791
                     /locus_tag="KRL67_003766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIBC"
                     /protein_id="HBH1524598.1"
                     /translation="MNKKIVAVCESERSLNSLKTAANDLGFDLVYEIQENDNIKNELS
                     IYDIEDASIVLFVTSDSIESIEKIDRFIDREFYEVDPKYIIEDAKSIVSEILIDLN"
     gene            complement(969..1664)
                     /locus_tag="KRL67_003767"
     CDS             complement(969..1664)
                     /locus_tag="KRL67_003767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FadR family transcriptional regulator"
                     /protein_id="HBH1524599.1"
                     /translation="MKSLNATEKIIDYIKLNILNGTFKINSKLPSERKIADLFNISRI
                     PVRNAINILCKEGILRAVPYSSPIVEGFKKVDLFDDGEIYKNHNIQEFYVESLRARQL
                     IESEATRLAILNATSKELQKIKYTYLKSIEELDKVSHGLIEECYDADLQFHKEIILTS
                     HNPIFIKYYELIPKTIFSNQYFGFKYRNSLKDMISHHNNIIIAFDSKDSNLGYTSMYN
                     HLEDVIQLFQHDD"
     gene            complement(1690..2877)
                     /locus_tag="KRL67_003768"
     CDS             complement(1690..2877)
                     /locus_tag="KRL67_003768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBH1524600.1"
                     /translation="MKKISNQKLLLLSIVTFFWFSQYVYIPFQNPYLSACNVSTNMIG
                     IIIGAYGISQCLLRLPVGVFADSINKHKIFILSGIFFAGLASLIRVFSPGGNGFLIAN
                     ILSGISSSTWISYMVHYTNFFSMEHQQKATSTIILVNNLGMLIGFVMSTLFYEKLGMT
                     ALCIMSVLSSVIGTILALNIKNYNFKKMPEKISIKKYLSICKDKRLIFFSFMALIQQG
                     IQMSTTMSFTTQILKNLGSSSTLIGLSSIFYMVCAVASSSFASTKFCIKYGPKFWIPL
                     VFILVSAYCFLVPTIKNINIIFVLQVLPGISTGILLSYLTSESMKNVPKHQKSTAMGF
                     FQAIYAIGMSVFPMIVGKISEYTSIQYGYFILAIIAMVASILSYTYYKKVDLTLNYDI
                     IQS"
BASE COUNT         1122 a          349 c          456 g         1032 t
ORIGIN      
        1 taacttagaa tatatatttt atttaattat cctaaagtga gaaaggaaga tgtgacatga
       61 ataaaaagaa aatattagtg ccaattgatg ggacagaaag aagtatgcat tctttggaat
      121 ttataaaagg aatattcaaa aaagatgaag ttgaagtaga aataatgaat gtaaaagaac
      181 tagtatttat tgatggcata tccctggctg aagagataaa aaattctgaa aacttaggaa
      241 gaagaatctt ggataaagca gcagagataa tgggagagta tgatgttaaa gtacatttta
      301 cttttggata tccaggagat gagataatta gaaaagcaaa agaagataat gtagatttta
      361 tagttatgac aaaatctact aaaaaaggtc ttacaagaat gataggttca gtaacagcaa
      421 gtgtagttaa acaagcaaaa tgtatagtga tgattgtacc ggagtagaat tagaaaggta
      481 tttgaaatat gaataaaaag atagtagcag tttgtgaaag tgaaagaagt ttaaattctc
      541 ttaagacagc tgcaaatgat ttaggatttg atttagttta tgaaatccaa gaaaatgata
      601 atataaaaaa tgaactttct atttatgata tagaagatgc atctatagtg ttatttgtta
      661 caagtgattc tatagaaagc atagagaaga ttgatagatt tatagataga gaattttatg
      721 aagttgatcc aaagtatata atagaggatg caaaatctat agtaagtgaa attttaatag
      781 acttaaatta atcatgggtg actcaaactg tgccttaaaa tgaacttttt agttcatttt
      841 aaggcacttt ttttctatga aatcaaatat atttttattg tttcaacaat gtgattattt
      901 ctatttttac acaagtcttt gagataattt atttttgtat gatagttgta tttaaaggaa
      961 aaagaagttt aatcatcatg ctgaaatagc tgaataacat cttctaaatg attgtacatt
     1021 gatgtataac ctaagttgga gtctttacta tcaaaggcta ttattatgtt attatgatgg
     1081 ctaatcatat cttttagaga atttctgtat ttaaaaccaa agtattgatt agagaaaatt
     1141 gttttaggta ttaactcata atactttata aaaattgggt tatgagaggt taaaattatc
     1201 tctttgtgaa attgcaagtc agcatcataa cattcttcta tcaaaccatg agaaacctta
     1261 tccaattcct ctattgactt taggtaagta tattttattt tttgaagttc tttagatgta
     1321 gcatttaaaa ttgctagtct agtagcttca ctttcgataa gttgtcttgc tcttaaagat
     1381 tctacataaa attcttgtat gttatgattt ttatagattt caccatcatc aaataggtca
     1441 acttttttaa atccttcaac tataggactt gaataaggca cagctctaag tattccttct
     1501 ttacagagta tatttatggc atttcttaca ggtattctac ttatgttaaa taggtcagca
     1561 atctttctct cacttggcaa tttactatta attttaaaag ttccatttag aatgtttaat
     1621 ttaatataat caataatttt ttcagtggca tttaagcttt tcataaaaac tcctaaacat
     1681 ataatttttt tatgattgta ttatatcata gttaagagtt aaatctactt ttttataata
     1741 tgtataagac aggatagagg ccaccatagc tataatggcc aagataaagt atccatattg
     1801 tattgaagta tattccgata ttttaccaac tatcattgga aacacagaca ttcctatagc
     1861 ataaattgct tgaaaaaaac ccattgcagt agatttctga tgttttggta catttttcat
     1921 agactcgcta gtaaggtaag aaaggagtat acctgtagat atacctggta gcacttgtag
     1981 tacaaatatt atattaatat tttttattgt tggaactaaa aagcaataag ctgaaactaa
     2041 aataaaaact aaaggaatcc aaaattttgg tccatattta atacaaaatt tagtagaagc
     2101 aaagctagaa gaggcaacag cacacaccat gtaaaatata gaagatagac ctataagggt
     2161 agaactagaa cctaagtttt taagaatttg agtagtaaat gacattgttg tagacatttg
     2221 aataccctgt tgaattaatg ccataaagga aaaaaatata agtcttttat ccttgcagat
     2281 agataaatac tttttaattg atattttttc aggcattttt ttaaaattat aattttttat
     2341 atttaaggct agaatagttc caataactga agacaataca ctcatgatac ataacgctgt
     2401 cattcctaat ttttcataaa ataaagtact cataacaaat ccaatcaaca tccctagatt
     2461 gtttactaat attatcgtac ttgtagcttt ttgttgatgt tccatagaaa aaaagttggt
     2521 atagtgaacc atgtaagaaa tccaagttga tgatgatatt ccagataaaa tgttagctat
     2581 aagaaaacca ttgcctccag gagaaaagac tcttataaga gatgctaaac cagcaaagaa
     2641 aattccagac agtataaata ttttatgttt atttatggaa tcagcaaata ctccaacagg
     2701 aagccttagt aaacattgcg agattccata tgcacctatt attataccaa tcatatttgt
     2761 agatacattg catgcactta aatatggatt ttggaaaggt atatatacat attgtgaaaa
     2821 ccagaaaaat gtaacaatag aaagtaaaag taatttttga ttagatattt ttttcatatt
     2881 atcctccata tggtattttt ataatgcaaa taatatacca tatggatttt ttaatcaagt
     2941 attttctcta ttaaaaaaa
//
LOCUS       DAEPXF010000293         2929 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain HB27
            SAMN13376389-rid12479423.denovo.301, whole genome shotgun sequence.
ACCESSION   DAEPXF010000293 DAEPXF010000000
VERSION     DAEPXF010000293.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13376389
            Sequence Read Archive: SRR10518432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2929)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2929)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001069624.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001069624.1
            Genome Coverage       :: 315x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/15/2021 01:54:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,165
            CDSs (total)                      :: 4,076
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 89
            rRNAs                             :: 10, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 10 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 19 of 69
            Pseudo Genes (incomplete)         :: 44 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 10 of 69
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2929
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13376389-rid12479423.denovo.301"
                     /strain="HB27"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="China: Hebei"
                     /collection_date="2017"
     gene            133..1419
                     /locus_tag="KRL67_003769"
     CDS             133..1419
                     /locus_tag="KRL67_003769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006807454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine/sarcosine/betaine reductase component B
                     subunit"
                     /protein_id="HBH1524601.1"
                     /translation="MKLELGNIFIKNVEFGEKTEVKDGVLYISSEEIEKIALEDERIV
                     SVNVELARPGESIRIAPVKDVIEPRVKIGDESKIFPGIINKVKTVGSGRTHVLLGACV
                     VTCGNIVGFQEGVIDMSGPTAKYTPFSKTNNICIVIKAKDGIDTHDYEEAARIAGLKI
                     GAYVGEAGREVEPDEVIVYETKPLLKQVKEYPDLPTVAYVHMLQSQGLLHDTYYYGVD
                     AKQIVPTFMYPTEIMDGAIISGNCVAPCDKVTTFHHLNNPVIHDLYKRHGKDLNFIGV
                     ILTNENVFLVDKERSSDMVAKLIEFLGVDGVLVTEEGYGNPDTDLMMNCKKCSEVGAN
                     VVLITDEFPGKDGKSQSIADATKEADAVVSCGQGNLVVHFPAMEKIIGTLDYVEMMIG
                     GYKGCLNEDGSMDAELQIIIASTIANGYNHLTARYY"
     gene            1505..2812
                     /gene="grdB"
                     /locus_tag="KRL67_003770"
     CDS             1505..2812
                     /gene="grdB"
                     /locus_tag="KRL67_003770"
                     /EC_number="1.21.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012158817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:2552..2554,aa:Sec)
                     /transl_table=11
                     /product="glycine reductase complex selenoprotein B"
                     /protein_id="HBH1524602.1"
                     /translation="MSKIRVVHYINNFFAGIGGEEKADIPPEKRAGVVGPGIALQSQF
                     NDDAEIISTVICGDTYFGENIESATQELLKMIKEESPDLFIAGPAFNAGRYGVACGSI
                     CKAVEEELNIPVVTGMYKENPGVDMFKLDLHIVSTGNSAASLRKVVPIMTKLGLKLVR
                     GEEIGPPEEEGYIMRGIRKNFFHELRGSERAIDMLVKKMKGESFETEYPMPEFDRVTP
                     MKPVKDLSKIKLALVTSGGIVPIDNPDKIESSSATKYGIYDLTDMDSMSNKEFTTIHG
                     GYDRAYVLENPNLVVPLDVVRELEKEGVIGELVDYFITTTGTGTSVGNSKRFGEEFSK
                     KLLEDDVDAVILTSTUGTCTRCGATMVKEIERAGIAVVHICTVVPISLTIGANRIVPA
                     IGIPYPLGNPMLGEEESKKIRKNIVLKALNALTEDIEEQTVFE"
BASE COUNT         1126 a          321 c          617 g          865 t
ORIGIN      
        1 aaaatgaaat tttctatgac attaatacat agaatatata ctttaaataa ttggcataga
       61 aaatgcttta tatattaata agcgtaataa atacttttaa ttgcgattaa atatattaag
      121 ggggattttc atatgaagct agagctaggg aatatattta taaaaaatgt tgaatttgga
      181 gaaaagacag aagtaaaaga tggagtacta tatattagta gtgaagaaat agaaaagata
      241 gcattagaag atgaaaggat tgtaagtgta aatgttgaac ttgcaagacc tggcgaatca
      301 ataagaatag ctccagttaa agatgtaata gagcctagag ttaaaattgg agatgaaagc
      361 aaaatatttc ctggaattat aaataaagtg aagactgtag gcagtggaag aacccatgta
      421 cttttaggag cttgtgttgt aacttgtgga aatatagttg gatttcaaga aggtgtaatt
      481 gatatgagtg gtcctactgc taaatataca cctttttcaa aaacaaataa tatatgtatt
      541 gttataaaag caaaggatgg tatagatact catgattatg aagaagcagc tagaattgca
      601 ggattaaaaa taggtgcata tgtaggtgag gcaggaagag aagttgagcc agatgaagtc
      661 attgtatatg agacaaagcc attattaaaa caagtaaaag aatatccaga cctgccaaca
      721 gtagcatatg ttcacatgtt acaatcacaa ggacttttac atgatactta ttactatgga
      781 gtggatgcta aacagatagt gcctacattt atgtatccaa ctgaaataat ggatggagct
      841 ataatatctg gaaactgtgt agcaccatgt gataaggtta caacattcca tcatttaaat
      901 aatccagtta tacatgactt atataaaaga catggaaaag atttaaactt tataggagtt
      961 attttgacta atgagaacgt attcttagtc gataaagaga gaagctctga tatggtagct
     1021 aaattaatag aatttttagg cgttgatgga gtcctagtga cagaagaagg ttatgggaat
     1081 ccagatactg acctaatgat gaattgtaaa aaatgtagtg aagttggagc aaatgtagtt
     1141 ttgataacag atgaatttcc tggaaaagat ggcaagagtc aatctatagc agatgctact
     1201 aaggaagcag atgcagttgt ttcttgtggt caaggaaatt tagtggttca tttcccagca
     1261 atggaaaaaa ttataggaac tttagattat gttgaaatga tgataggtgg gtataaagga
     1321 tgcttaaatg aagacggaag catggatgca gagctacaaa ttataatagc atcaactata
     1381 gcaaatggat ataatcattt gacagcaaga tattattaaa taagtatttg aatataaagt
     1441 tctgttgatg taatcattgg tctaattgat gtaataacta taaagtgatt tggaggatta
     1501 agagatgagt aaaatcagag tggttcatta tataaacaat ttctttgctg gtataggtgg
     1561 agaggaaaaa gcagatattc cgccagaaaa aagggctggt gtagtaggac ctggaatagc
     1621 acttcagagt caatttaatg atgatgcaga aataatctca actgtaattt gtggagatac
     1681 gtactttggt gaaaatattg agagtgcaac tcaagaactt ttaaaaatga taaaagaaga
     1741 aagcccggat ttatttattg caggcccagc atttaatgca ggtagatatg gagtagcttg
     1801 tgggagtatt tgtaaggcag ttgaagaaga gctaaatatt cctgtagtta caggaatgta
     1861 taaagaaaat ccaggtgtag atatgtttaa attagatttg catatagtgt ctacaggaaa
     1921 ttcagcagca agtcttagaa aggtagttcc aataatgacg aaactaggtc ttaaattggt
     1981 tagaggtgag gaaataggac ctcctgaaga agaaggatat ataatgagag gaataagaaa
     2041 aaatttcttc catgaactta gagggtctga aagagcaatt gatatgttgg ttaagaagat
     2101 gaaaggtgaa agctttgaaa ctgagtatcc aatgcctgaa tttgatagag ttacacctat
     2161 gaaacctgtt aaagatttat ctaagataaa acttgcccta gtaacttctg gagggatagt
     2221 tccaattgat aatccagaca aaattgaatc ttcaagtgct actaaatatg gaatttacga
     2281 tttaacagat atggattcta tgtcaaataa agaatttaca actatacatg gggggtatga
     2341 tagagcatat gtccttgaaa atccaaattt agttgtacca ctagatgtag ttagagagct
     2401 tgaaaaagaa ggtgtaattg gagaattagt agattatttt ataactacta caggaacagg
     2461 aacgagtgtt ggtaattcta agagatttgg agaagagttt tctaagaaat tgttagaaga
     2521 tgatgtagat gctgtaattt taacttctac atgaggtact tgtactcgtt gtggagcaac
     2581 gatggtaaaa gagattgaga gagctggtat agcagttgtc catatatgta cagtagtacc
     2641 aatatcactt acaataggag ctaatagaat tgttccagcc atagggatac catatccact
     2701 tggaaatcct atgttaggag aagaggaaag taaaaaaatt agaaagaata tagttttaaa
     2761 agctttaaat gcactaacag aagatataga agaacaaaca gtatttgaat aataaaaata
     2821 caacacaaca ctgaaataaa aaaagatatc aaaaaataat tttttaaaat tattttacga
     2881 tatctttttt tattagataa attcgaaatt tgcatattat ttgcatatt
//
LOCUS       DAEPXF010000294         2924 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain HB27
            SAMN13376389-rid12479423.denovo.302, whole genome shotgun sequence.
ACCESSION   DAEPXF010000294 DAEPXF010000000
VERSION     DAEPXF010000294.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13376389
            Sequence Read Archive: SRR10518432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2924)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2924)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001069624.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001069624.1
            Genome Coverage       :: 315x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/15/2021 01:54:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,165
            CDSs (total)                      :: 4,076
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 89
            rRNAs                             :: 10, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 10 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 19 of 69
            Pseudo Genes (incomplete)         :: 44 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 10 of 69
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2924
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13376389-rid12479423.denovo.302"
                     /strain="HB27"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="China: Hebei"
                     /collection_date="2017"
     repeat_region   160..579
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=227..255
                     /rpt_unit_seq="atttacataccacatagttaatataaaac"
     gene            1224..2495
                     /locus_tag="KRL67_003771"
     CDS             1224..2495
                     /locus_tag="KRL67_003771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HBH1524603.1"
                     /translation="MLKVLKGNIIFTKTSETFTVFENSYIILEKGKVKGIFKEIPKEY
                     KNLKVVDYTDKLIIPGMNDLHAHASQFKNLGMAMDKELLPWLETYTFPEESNYKDIEY
                     ATKMYKKFVKELWKSGTTRIAVFATVHKEASMKLMDIFKEVGLGAYVGKVNMNMNCPD
                     TLLENTQDSIADTEEIINKYSDTDSIVKPIITPRFIPSCTSELLKGLGDLCLKYNIPI
                     QSHLSENYDEIEWVRNLEPESKFYGDAYNRYNLFGQTPTLMAHCVHCSQDEIDLMREN
                     NVMAVHCPASNFNVGSGAMPIRKLIDNNIRLALGSDISGGHTLSIFKAMVSAIQLSKL
                     YWVNSGKKYNFLSLSEAFYIATKSGGSFFGKVGSFEEGYDFDALIIDDSNLNHDNYSI
                     LERLERFIYVGDDRNIIHRYVCGKLIEEPNI"
BASE COUNT         1142 a          372 c          383 g         1027 t
ORIGIN      
        1 caatactttt tataattatt ctacttttat attttaatca ttaaaactaa agtcaatgtt
       61 atctggtaat tatttactac aaatgataaa aaataattat attttagggg agaaaatgct
      121 atataaatta ttggggtatt ttacctatac ttgttgcaaa tttacatacc acgtagttaa
      181 tataaaacgt ggtggtgata tgggaggtgt aattaaaggt tataaaattt acataccaca
      241 tagttaatat aaaacagaac ttctactcct cgaccaccac cactgttgaa aatttacata
      301 ccacatagtt aatataaaac gaattagaat ctggtgcagt tgatgaagtc ggtcaaattt
      361 acataccaca tagttaatat aaaaccaagt tacagtattt aatttcatag ataagttatt
      421 atttacatac cacatagtta atataaaacg gagtatttca taacagattt tgaaacagat
      481 atagaattta cataccacat agttaatata aaacaataaa tatatgtgac cttttagaag
      541 gtaaaacaat atttacatac cacatagtta atataaaacc ccaaaataaa ctaagtattt
      601 tcagtactta cacacataca actctctcaa atttgcagtg aaccatgagt agtgcaattg
      661 ataacattta tcatacaccc tcaatccctt acattgcaac tgttaaaccc tactttatca
      721 aaaatatcgc tcactgcaaa atttatatat ttttattata tcataaaatt caaactataa
      781 aaataaaatg gaataattta tcaatttaag aacacctgca tatctgtaaa tacttactat
      841 attatctatt ctaatagttc ttcttctagc tggaactaat ataaaaatca tctctgaaag
      901 attaggacat actgatataa aaataactat gaatagatat tttcttattt tatatgatat
      961 ggataaagaa gcttctgaaa atataatcaa ggttatcttt aaataatgtt cagttttatg
     1021 tcagtaagta tcgtattttt cttaaatgtc acttagatat tcagttatat ataatagtaa
     1081 acaactgtat ttcactaata tttgctatta tctaatagta tttatagttt agatttgata
     1141 taatacatgt ggtaaaattt tgatttgtaa aacaaaatat aacctacata tattaaaaca
     1201 aatttaaaac taaggagaac tatatgttaa aagtactaaa aggaaatata atttttacaa
     1261 aaacttcaga aactttcaca gtatttgaaa atagttatat tatactggaa aaaggcaaag
     1321 taaaaggaat atttaaagag atacctaagg aatacaaaaa cctaaaagta gtagattaca
     1381 ctgataagct tataatacct ggtatgaatg acctccatgc ccatgcctct caatttaaaa
     1441 atcttggaat ggctatggat aaagagcttt tgccatggct tgaaacttat acatttccag
     1501 aagaatcaaa ttataaagat atagaatatg caacaaaaat gtataaaaaa tttgtaaaag
     1561 aactttggaa atctggaact acaagaatag cagtgtttgc aacagtacat aaagaagcct
     1621 ctatgaaact tatggatata ttcaaagaag ttggtcttgg agcatacgtt ggcaaagtaa
     1681 atatgaatat gaattgccct gatactttat tagaaaacac tcaagattct attgctgata
     1741 cagaagaaat tataaataag tatagtgata cagattctat tgtaaaacca ataataacac
     1801 ctagatttat accaagctgt acaagtgaac ttttaaaagg tttaggagat ttatgtttaa
     1861 aatataatat tcctatacaa tcacacttat ctgaaaatta tgatgagatt gaatgggtta
     1921 gaaatctaga acctgaatct aaattctatg gagatgcata taatagatat aatttatttg
     1981 gtcaaactcc tactttaatg gctcattgtg ttcattgttc tcaggatgaa atagatttaa
     2041 tgagagaaaa taatgttatg gcagttcact gtccagcttc taactttaat gttggtagtg
     2101 gtgctatgcc tatcagaaaa ttaatcgata ataatattag attagcactt ggctcagata
     2161 taagcggtgg acatacattg tcaatattta aagcaatggt atctgcaata caattatcta
     2221 aattatattg ggttaattct ggtaagaagt ataatttctt atctttatct gaagcttttt
     2281 atattgcaac caaaagtggt ggaagtttct ttggaaaagt tggtagcttt gaggaaggtt
     2341 atgattttga tgcattgatt attgatgatt caaatttaaa tcatgacaat tattctatac
     2401 ttgaaagact tgaaagattt atctatgttg gtgatgatag aaatatcatt catagatatg
     2461 tatgtggaaa actaattgaa gaacctaata tataattata ataaaaattt taattgtata
     2521 atcttgttaa tttatacaac tataattaat aaaagagtat attttaaatc taaaagctat
     2581 aggttgaaac atactctttt tattttgttt atttatagct ttagtgatta atcatatata
     2641 aatctacaaa atgtattcct agtaacttac attttttgat atccacaatc ataaaactat
     2701 attattattg gattcatacc tcaaaaatat cgccttctgg acactattat attcaaagct
     2761 atgttaattt gcaatgatat atctaacatg tattgattaa aaaaattaaa acttgctttt
     2821 tatctcctat atctaaagaa gtggcattta gaataaacta ttgtaatatt aaagttgaaa
     2881 ttttctagtt tatttactct atagcaccat attataatac tatt
//
LOCUS       DAEPXF010000295         2894 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain HB27
            SAMN13376389-rid12479423.denovo.303, whole genome shotgun sequence.
ACCESSION   DAEPXF010000295 DAEPXF010000000
VERSION     DAEPXF010000295.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13376389
            Sequence Read Archive: SRR10518432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2894)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2894)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001069624.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001069624.1
            Genome Coverage       :: 315x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/15/2021 01:54:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,165
            CDSs (total)                      :: 4,076
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 89
            rRNAs                             :: 10, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 10 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 19 of 69
            Pseudo Genes (incomplete)         :: 44 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 10 of 69
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2894
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13376389-rid12479423.denovo.303"
                     /strain="HB27"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="China: Hebei"
                     /collection_date="2017"
     gene            28..918
                     /locus_tag="KRL67_003772"
     CDS             28..918
                     /locus_tag="KRL67_003772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HBH1524604.1"
                     /translation="MAKLILEHIKGNTYYIPLPTIVGVYVDGKDAILIDSGNNKDTAR
                     QVLRLLEEHNLSPKLIINTHSNADHIGGNAYLKNQTKCKIATTKIEGYFTENPILESA
                     FLYGGYPSKALKNKFLLAKESEVDYIIPSNGKIVDTELEAISLPGHYFEMIGVRTPDN
                     VLFVADSLTPENIITKYHFFFLLDIDSQFKTLEKLRMLEADFFVPSHSVKTTDIKSLI
                     NINKKKMEEIIDNIKKVCYQPVMIDEVIEKMCDLYNVKLDANQYVLVGSTIRSYITYL
                     YENNMVEYIFDGGKMMIKAV"
     gene            1136..2890
                     /locus_tag="KRL67_003773"
     CDS             1136..2890
                     /locus_tag="KRL67_003773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp23"
                     /protein_id="HBH1524605.1"
                     /translation="MYIMNKKFKKFSKKLSILILVLTMFLFNNLTANAQIKEDSIIGI
                     DRYETAGLIADRQTYDTVILVNGDKNLSDGLSSSGLAGAINAPILLTKKSEIPKATLN
                     RLDNKNLNVVKKVYIIGGYNTIENSVEKDIKRKGIQVERINGNNRIETSYNVAKKINE
                     VSSVKEVMLTNGFVGEADAMSIAPVVSKNKGAIILTDGKSVPFKTEGLNIYAIGGKSA
                     INEDLVNETKATRIGGNDRFETNKKVMEKFYNGATDFYITNGYQLVDALTLSPLAKEK
                     PIVLVADGSNKGILKGAKSITKVGGIDANIYKQCLDMVKYNNMDITPNILKHLTSVEG
                     NEVSEILIESDNLGVVVEKTNTNKFEFDYVSVTNEKNCTFSVYKENSSNNVKNGKLVV
                     SAKKKIDKKDSDLQDMNGDNMINANKDRMVNVIKIGVPDKMYSNFKVDVESGSVELYN
                     IKGGATVDVNDGIANIVDNNVTNPFNINTNSGISGVTAETISSEIKFRANNGIVNVTA
                     TNINGNISLFGSDNKDTFDGIFKLNLKKEPSNLHLKLIGNGINKLPEGWSKDYILGNG
                     QPIIEVKNNGINNISIGE"
BASE COUNT         1205 a          271 c          512 g          906 t
ORIGIN      
        1 aaagtaaatt taaagggagg ttataaaatg gctaaattaa ttctagaaca tataaaagga
       61 aatacatatt atattccact ccctacaata gtaggtgtat acgtagatgg taaagatgca
      121 attttaattg atagtggaaa caataaggat acagctagac aagttttaag attattagag
      181 gaacataatt taagtcctaa attaataata aacactcatt caaatgcaga ccatatagga
      241 ggaaatgcat atttgaagaa tcaaacaaaa tgcaagattg ccacgactaa aattgaagga
      301 tattttacag aaaatccaat attagaatca gcatttttat atggtggcta tccaagtaaa
      361 gcactgaaga ataaattttt attagcaaag gaaagtgaag ttgattatat tataccttca
      421 aatggcaaaa ttgtagatac agaacttgaa gcgatttctc ttccaggtca ttattttgaa
      481 atgattggtg ttagaactcc agataatgtt ttgtttgttg cagatagtct tacaccagag
      541 aatataatta ctaaatatca ttttttcttc ctactggata tagattcaca gtttaagaca
      601 ctagaaaaat tgagaatgtt agaggcagat ttctttgttc caagtcactc tgttaaaact
      661 acagatataa aaagtttgat taatattaat aagaagaaga tggaagaaat aatagataat
      721 attaaaaagg tttgttatca gccagttatg attgatgaag ttattgaaaa aatgtgtgat
      781 ttgtacaatg tcaagcttga tgctaatcaa tatgtgttgg ttggaagtac aatacgttct
      841 tatataactt atttatatga gaataatatg gttgaatata tatttgatgg tggtaagatg
      901 atgataaaag ctgtgtaaat agtaataatt ttcaaattat atgatgaaat tgttttagta
      961 tgactgttat ttgaaaagta atataaaaaa atttatataa ataagctaag atgattgata
     1021 taaagttact gataatttta aggtaatata tacgttacta taatattatg ttattatttg
     1081 taaataaggg gataatttat aagcaaatta aagaccttaa tttacaggag gaatagtgta
     1141 tattatgaat aaaaaattca aaaaattcag taaaaaacta agtatattaa ttttagtttt
     1201 gactatgttt ctttttaata atttaactgc aaatgcacag attaaagaag attcaataat
     1261 aggaattgat agatatgaaa cagcaggact tatagcagat aggcaaactt atgatactgt
     1321 aatattagtt aatggagata aaaatctatc tgatgggctt tcatcaagtg gattagctgg
     1381 agctattaac gcaccaatac ttttgactaa aaaaagtgaa ataccaaaag ctactttaaa
     1441 tagactagat aataagaatt taaatgttgt aaaaaaagta tatataattg gtggttataa
     1501 tactattgaa aattcagtag aaaaagatat aaaaagaaaa ggtattcaag tggaaagaat
     1561 aaatgggaac aatagaattg aaactagtta taatgtagcc aaaaaaataa atgaagtaag
     1621 tagtgtaaaa gaggtaatgc ttacaaatgg atttgtagga gaggctgatg ctatgagtat
     1681 agcacctgta gtttcaaaaa ataaaggagc tataatactt acagatggga aatcagttcc
     1741 atttaagact gaaggtttaa atatttacgc tataggcggc aaatctgcga taaatgagga
     1801 tttagtaaat gaaactaaag caactagaat cggaggaaat gatagatttg agaccaataa
     1861 aaaggtaatg gaaaagtttt ataatggagc gacagatttt tatataacaa atggatatca
     1921 attagtagat gcactaacat tatctccttt agccaaagaa aaaccaatcg ttttagttgc
     1981 tgatggaagt aataaaggta ttttaaaagg agctaaaagt ataacaaaag ttggaggtat
     2041 tgatgccaat atatataaac agtgtttaga tatggttaaa tataataata tggatataac
     2101 tccaaatatt ttaaaacact taacttcagt tgaaggaaat gaagtttctg aaatacttat
     2161 agaatcagat aatttaggtg tggttgttga aaagacgaat accaataaat ttgagtttga
     2221 ttacgtaagt gtaactaatg aaaaaaattg tactttttct gtatataaag aaaattcttc
     2281 taataatgta aaaaatggaa agctagttgt aagtgctaag aaaaaaatag ataagaaaga
     2341 tagtgatttg caagatatga atggcgacaa tatgataaat gcaaataaag ataggatggt
     2401 aaatgtgata aaaattggag ttcccgataa aatgtattct aattttaaag tagatgttga
     2461 aagtggttca gtagaacttt ataatattaa aggaggagct actgttgatg tcaatgacgg
     2521 aattgctaac attgttgata acaatgtaac aaatcctttc aatataaata ctaatagtgg
     2581 gatttcaggt gtaacagctg aaactatatc atctgagata aaatttagag caaacaatgg
     2641 aattgtaaat gtaacagcta caaatataaa tggcaatata agtttatttg gaagcgataa
     2701 taaagatacc tttgatggaa tatttaaatt aaatctgaaa aaagaaccat caaatttaca
     2761 cttgaagtta attggaaatg ggataaataa attgccagaa ggttggtcta aagattatat
     2821 tttaggaaat ggacaaccaa ttattgaagt taaaaataat ggcataaata atatatcaat
     2881 tggagaataa aaac
//
LOCUS       DAEPXF010000296         2894 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain HB27
            SAMN13376389-rid12479423.denovo.304, whole genome shotgun sequence.
ACCESSION   DAEPXF010000296 DAEPXF010000000
VERSION     DAEPXF010000296.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13376389
            Sequence Read Archive: SRR10518432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2894)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2894)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001069624.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001069624.1
            Genome Coverage       :: 315x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/15/2021 01:54:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,165
            CDSs (total)                      :: 4,076
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 89
            rRNAs                             :: 10, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 10 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 19 of 69
            Pseudo Genes (incomplete)         :: 44 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 10 of 69
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2894
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13376389-rid12479423.denovo.304"
                     /strain="HB27"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="China: Hebei"
                     /collection_date="2017"
     gene            266..643
                     /locus_tag="KRL67_003774"
     CDS             266..643
                     /locus_tag="KRL67_003774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HBH1524606.1"
                     /translation="MMIRKIPQAELKVMKFIWKVDVTVTSKDVIEAMEQKYGWKQTTT
                     LTLLSRLVKRGFLDAQKIDRYTHYTIRVKHKDYLSFETKDFLSNIHDDSLRSLISALH
                     DDENIGKDKLDFLESYFDNLKDE"
     gene            636..1658
                     /locus_tag="KRL67_003775"
     CDS             636..1658
                     /locus_tag="KRL67_003775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease"
                     /protein_id="HBH1524607.1"
                     /translation="MNNYMLYVFENLIKTTIVCSLGICLLLFLKRYLFKKFSKRFNYY
                     IWLIVVFRMLLFLFNYTIVYEVKESKENTVGNNITQIDISTDNNLMLYVAYLWLFVTI
                     VIAVYTFIKYTRFKNLVVDVSYDIEDNDINCIYKNLLKELNIKKKIELRGSDELISPA
                     GMGLFKSYIFLPDYPYSKDELTWILKHELMHFKNKDILIKFLVLSVRIIYWFNPLVYV
                     MSNKVNLDCELCCDESVIYNCSFNDKKEYALALIKSIKLSKECNRGILLTDFNRTNLE
                     KRLESIISKKFKNGVFVAIILCLLSSITYFKFDIFISKPVIYSLPKPSVLNDEYIYNT
                     DIRVER"
     gene            1720..2313
                     /locus_tag="KRL67_003776"
     CDS             1720..2313
                     /locus_tag="KRL67_003776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding protein"
                     /protein_id="HBH1524608.1"
                     /translation="MLKKIKKVLSIACCLFILGGSISFADLENNNEINDKSVENPIYE
                     FNIQGYTFTYENAPQRIKDEYNKTCRELNITPSPSTEIFIPIDEISKYTDTVPLFEID
                     EFNVKYFGTRFEVTGAKTYTVGVSTYVGYGHTTSGNPVHLAQLLLKKSGYSIAIDSLF
                     GSSTYSKVRSFQSKYGLSVDGVVGLGTWNKLAQVTNA"
BASE COUNT         1142 a          277 c          419 g         1056 t
ORIGIN      
        1 agtattataa tattctttgt gtgaaaatat aggaattttt attaaatagt tttaatgtgt
       61 aaataaacat tctacactat tgtaattaaa tatgtaaaaa gtaaaatatt taaatggata
      121 tattttaaaa tctaactttt aatattgtaa aaaaattaga aatttaacaa aaagtacaaa
      181 aattcacatt tttttcataa agtgtttgac taaaaaattc acatgttgta gaattaaggt
      241 agcaggagtt ttgagggagg tgtaaatgat gataagaaaa ataccacaag cagaactaaa
      301 agtaatgaaa ttcatttgga aagtagatgt tacagtgaca tcaaaagatg ttattgaagc
      361 tatggaacaa aaatatggct ggaaacaaac caccacactg acacttttat ctaggctggt
      421 aaaaagagga ttcttagatg ctcaaaaaat agacagatat acacattaca caataagagt
      481 aaaacacaaa gattatttaa gttttgagac aaaggatttc ctaagtaata tacatgatga
      541 ctctttaaga agcttaattt cagcattaca tgatgatgaa aatataggta aagataagct
      601 agattttcta gaaagttatt ttgataattt aaaggatgaa taattatatg ctatatgttt
      661 ttgaaaatct tataaagaca actattgtat gtagtcttgg tatttgtcta ttattatttt
      721 taaaaagata tttatttaag aagtttagta agagattcaa ttattatatt tggttgatag
      781 tagtctttag aatgttactt tttctattta attatacgat agtgtatgaa gtaaaagaat
      841 ccaaagaaaa tacagtagga aataatatca cacaaattga tatttctaca gataataatc
      901 ttatgctgta tgtagcttat ttatggttat ttgtaactat agtcattgca gtttatacat
      961 ttataaagta tacaagattt aagaatctcg ttgttgatgt atcttatgat attgaagata
     1021 atgatataaa ctgtatctac aaaaatttgt taaaagaatt aaatataaaa aagaaaattg
     1081 aattaagagg ttctgatgaa ttgataagtc cagcaggcat ggggttattt aaatcttaca
     1141 tattcttacc agactatcct tatagtaaag atgaactaac ttggattttg aaacatgaat
     1201 taatgcattt taaaaataaa gatattttaa taaagttttt agtgttatct gttagaataa
     1261 tatattggtt taatccactt gtttatgtaa tgagcaataa agtaaatcta gattgtgaat
     1321 tatgttgtga tgaaagtgtt atttataatt gttcatttaa tgacaaaaaa gaatatgcat
     1381 tggcacttat taaatcaatt aaactaagta aagaatgcaa tagaggtatt ctattaacag
     1441 attttaatag aaccaattta gaaaagagac ttgaaagtat tattagtaaa aagtttaaaa
     1501 atggtgtttt tgtagctata attttgtgtt tactatcttc aataacatat tttaaatttg
     1561 atatatttat ttcaaaacca gtgatttatt cgttaccgaa gccaagtgtg ttaaatgatg
     1621 aatatattta caacactgat atccgagttg aaagatgatg ttaaattgta ttaaaaaatg
     1681 taataattta aataaaagaa aatattttag gaggatttta tgttaaaaaa aattaaaaaa
     1741 gtactatcaa tagcgtgctg tttattcata ttaggaggga gtatatcttt tgcagattta
     1801 gaaaataata atgaaataaa tgataaatct gttgagaatc caatttatga atttaatatt
     1861 caaggttata catttacata tgaaaatgca ccacaaagaa ttaaagatga atataacaaa
     1921 acatgtagag aattaaatat aacaccttct ccatctactg aaatatttat tccaatagat
     1981 gaaatctcta agtatacaga tactgttcct ctttttgaaa tagatgaatt caatgttaaa
     2041 tattttggaa cacgttttga ggttactggt gcaaaaacat atacagttgg agttagtaca
     2101 tatgtgggat acggacatac aactagtgga aatccagtac atttagcaca gcttttacta
     2161 aaaaaatcag gatattctat agcaatagac agtttgtttg gtagttctac atatagtaaa
     2221 gttagaagtt ttcaaagtaa atatggacta agtgttgatg gtgtggttgg attgggaaca
     2281 tggaataagt tggcacaagt tactaatgct tagataattt attataaagc taaaaagaaa
     2341 catgggagtc tggaaatatt catgcatatt cttttaatga aattttattt ttaaaatgcc
     2401 aattaaatat atataaataa gactatgttt ataaaaatta tagtcttatt tatatattta
     2461 ttcaaaagtt attactcctt taaggaaaat ccagctagac taaatacata agttattaat
     2521 gtatttttaa ctcctattct gcaagtaatt ttaggtaatt ctttgaactt ttattttctc
     2581 taattaatat tactaatgta ttctttatat cttataagtt gatatcaata gctatacact
     2641 gtattattta ttattatact tttacttcta aatttataaa atgtttaatt cataaatata
     2701 attggataaa taatatcaaa tgttctattc tatcatccat gaatgaaagg tatacctata
     2761 tcagtaattt tagcaattag tgaatgagat gtatcaattc tgcaaatact tttaaggtaa
     2821 cttgaaatat ttaaggttat tttttcaaac tctgcttcta acaaattaat gaattgtact
     2881 tatgaataaa tatt
//
LOCUS       DAEPXF010000297         2879 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain HB27
            SAMN13376389-rid12479423.denovo.305, whole genome shotgun sequence.
ACCESSION   DAEPXF010000297 DAEPXF010000000
VERSION     DAEPXF010000297.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13376389
            Sequence Read Archive: SRR10518432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2879)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2879)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001069624.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001069624.1
            Genome Coverage       :: 315x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/15/2021 01:54:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,165
            CDSs (total)                      :: 4,076
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 89
            rRNAs                             :: 10, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 10 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 19 of 69
            Pseudo Genes (incomplete)         :: 44 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 10 of 69
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2879
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13376389-rid12479423.denovo.305"
                     /strain="HB27"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="China: Hebei"
                     /collection_date="2017"
     gene            63..1172
                     /locus_tag="KRL67_003777"
     CDS             63..1172
                     /locus_tag="KRL67_003777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HBH1524609.1"
                     /translation="MDFKSLGISENTINILKKSGITTPTPIQKESIKLIKEGKDVIAE
                     AQTGTGKTLAFLLPIFENISLDINDIQVLILSPTRELAIQITEEAMKLKESKDVSILA
                     AYGGKDIGSQIKKLKGNIHMIIATPGRLLDHLNRKTIDLSKLKTFVLDEADQMLLMGF
                     KNEVEAILKETSNKKQTLCFSATINSQVKKLAYRYTKNPVVVSIQKEEITLNNIKQEV
                     VETTDRKKLDALCKVLDEDNPFMAIIFCRTKRRVDNLEEALAIRGYNCQKLHSDIAQS
                     KRERIMKSFRNLDIQYLIATDVASRGLDISGVSHIYNYDLPETPEDYIHRIGRTGRAG
                     EEGYTCAFIDPKNERMLSEIETAIESKISRRIIEL"
     gene            complement(1550..2734)
                     /locus_tag="KRL67_003778"
     CDS             complement(1550..2734)
                     /locus_tag="KRL67_003778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH1524610.1"
                     /translation="MQKNVKKAKVTGSMLAIFGVASVLFSSHAGGGFATGNQETQYYV
                     QYGWTAPLMAILAMIILTATMREVIIMYNNNNCRNYKDLFCELWKPYPKLEIIWEIYY
                     YLMVLIAVSAVIAGAAAVFQSIGVNYFVAVFIIGVVLLVFTIFGAMLVSKAATAMTIA
                     ILVCTLTIFIVGIKAKVPEITEILSNRTSFTPGYIKPILNTFIYAGFQSVVIPTLAGC
                     SRPLLKNSKEATKAMIISFVMNAIALGLAVTMLMGWYREIIAAGQTTLPTLYVAGQSG
                     NHTIYIIYNVALFLCLMSTGVTTIFGLVNRFEDHKALSFLSSRMKRRVFTACAIMVVS
                     MLISLTGLSNIVKYGYGYCGYLGLFTIVIPFLTLGHYKNKKFAKENPEAKWPAELNEN
                     VN"
BASE COUNT         1098 a          441 c          398 g          942 t
ORIGIN      
        1 caaatatgat attattaatt gattaataaa aaataaaatt tttcatgaaa ggtaagataa
       61 caatggattt caaaagctta gggattagtg aaaatacaat aaatattcta aaaaaatcag
      121 gaataacaac acctacacct atacaaaaag aaagtattaa actaataaaa gaaggaaaag
      181 atgtaatagc agaagctcaa actggaacag gaaaaacact agctttttta cttccgatat
      241 ttgaaaatat atcattagat ataaatgata tacaagtact tatactttct cctacaagag
      301 aactagctat tcaaataaca gaagaagcta tgaaacttaa agaatctaaa gatgtaagta
      361 ttctagcagc atatggagga aaagatattg gttctcaaat aaagaaacta aaaggaaata
      421 ttcatatgat tatagctaca ccaggtaggc ttttagacca tctaaataga aaaacaatag
      481 accttagtaa gttaaagact tttgttttag atgaagctga ccaaatgttg cttatgggat
      541 ttaaaaatga agtggaagct attttaaaag agacttcaaa caaaaagcaa acactatgct
      601 tttctgcaac tataaattct caagtaaaaa aattagctta tagatacaca aaaaatcctg
      661 ttgtagtttc tattcaaaaa gaagaaatca ctcttaacaa tataaaacaa gaagtagtag
      721 aaactacaga tagaaaaaaa ttagatgcct tatgtaaggt gctagatgaa gataatcctt
      781 ttatggcaat aatattttgt aggactaaga gaagggttga caatcttgaa gaagctcttg
      841 ctataagagg atataattgt caaaaacttc atagtgatat tgctcaatca aaacgagaaa
      901 gaataatgaa atcatttaga aatttagata tacaatactt aatagctaca gatgtagctt
      961 ctcgtggtct tgatatatct ggagtaagtc atatatataa ttacgattta ccagaaacac
     1021 cagaagacta catccaccgt attggtagaa ctggtagagc tggtgaagaa ggctatacat
     1081 gtgcttttat tgaccctaaa aatgaacgaa tgttaagtga aatagagacg gctatagaat
     1141 ctaaaattag tagaagaatt atagaattat aatttataga ctacttaaaa taccaatgtc
     1201 acacaaacac tgtaatatat agaaagctac agaaaacatg tatcaatcta ttctttgctg
     1261 ttatactatt ttatattaaa gaaaaaagag gctatctaaa agttggatat gtctctttta
     1321 ttgtcgaaat aatgaaaata tatttgactt catataaaaa agagtgcctt ataaactaaa
     1381 agtccatttt aagacaccct ctttttatct tttattctta gttttatatt gcttgtttat
     1441 agttgtttaa tattagattt tgcaaataat cttaataaac atgcacatat attattcata
     1501 gtattcttat ttacagtatt ctctttagca atattttttg ctactacatc taatttacat
     1561 tttcatttaa ttccgctggc catttagcct ctggattttc ctttgcaaac ttcttattct
     1621 tataatgtcc tagtgtaagg aatggaatta ctatagtgaa tagaccaaga taaccacaat
     1681 atccatatcc atatttaaca atattactta atcctgtaag tgatataagc attgacacta
     1741 ccattattgc acaagctgta aatacccttc ttttcattct tgaagaaagg aaagaaagtg
     1801 ctttatggtc ttcaaatcta tttacaagac caaatatagt agttactcct gtagacatta
     1861 aacaaaggaa taatgcaaca ttataaatta tgtatatagt atgatttcca gattgacctg
     1921 caacataaag agtaggaagt gttgtttgcc cagcagcaat aatttctcta taccatccca
     1981 ttagcatagt tacagcaagc cctaatgcaa ttgcattcat aacaaatgaa attatcattg
     2041 ccttagtagc ttcttttgag ttcttaagaa gtggtcttga acatcctgca agtgtaggta
     2101 taacaaccga ttgaaaacct gcataaataa aagtattaag tattggcttg atatagccag
     2161 gtgtgaaact cgttctattt gataatattt cagttatttc tggtacttta gcttttatac
     2221 caacaataaa aatagtaaga gtacatacta gaatagctat tgtcattgct gtagctgctt
     2281 tagatacaag catagcacca aatatagtaa agacaagaag tacaactcca ataataaata
     2341 cagctacaaa ataatttacc cctatacttt gaaatactgc tgctgcacct gctataacag
     2401 cacttacagc tattaacacc attaaatagt aatatatctc ccatattatt tcaagttttg
     2461 gatatggttt ccaaagctca caaaataaat ccttataatt cctacaatta ttattattat
     2521 acattatgat aacttctctc atggtagcag taagtataat cattgctaaa attgccataa
     2581 gtggtgctgt ccatccatac tgcacataat actgagtttc ttgattccct gtagcaaatc
     2641 ctcctcctgc atgtgaacta aaaagaacgc ttgcaacacc aaaaatggct aacatagagc
     2701 ctgttacttt tgctttcttt acattttttt gcatgaaatc atcctcccta aaatgatatt
     2761 gaaagttttt attttaacac tttattgata aaacaataac atttttgttc attaaattca
     2821 accatatttt tccaatagtt ttacattttc aacttatttc tactcttata ttaaatatt
//
LOCUS       DAEPXF010000298         2876 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain HB27
            SAMN13376389-rid12479423.denovo.306, whole genome shotgun sequence.
ACCESSION   DAEPXF010000298 DAEPXF010000000
VERSION     DAEPXF010000298.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13376389
            Sequence Read Archive: SRR10518432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2876)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2876)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001069624.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001069624.1
            Genome Coverage       :: 315x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/15/2021 01:54:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,165
            CDSs (total)                      :: 4,076
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 89
            rRNAs                             :: 10, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 10 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 19 of 69
            Pseudo Genes (incomplete)         :: 44 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 10 of 69
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2876
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13376389-rid12479423.denovo.306"
                     /strain="HB27"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="China: Hebei"
                     /collection_date="2017"
     gene            73..146
                     /locus_tag="KRL67_003779"
     tRNA            73..146
                     /locus_tag="KRL67_003779"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:105..107,aa:Gly,seq:tcc)
     gene            155..271
                     /gene="rrf"
                     /locus_tag="KRL67_003780"
     rRNA            155..271
                     /gene="rrf"
                     /locus_tag="KRL67_003780"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            545..2563
                     /locus_tag="KRL67_003781"
     CDS             545..2563
                     /locus_tag="KRL67_003781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HBH1524611.1"
                     /translation="MRRNTKLLTTGILSMAIVTPTMAFATESNAMENNADLNINLEKK
                     SIVLGSTSKVSVKFKEKPDADSITLKYKCYDMPLDTTLNYNQSTESYEGTINYNKDPE
                     YLNVWELQGITINSKNNPKTLNKQDLENMGLNLKDYNVTQECIIEDITSRKDVNKYLR
                     KTSAPITELTGSDRYETAVKISKEGWKNGSDKVVIINGDVSIDGIISTPLATTYNAPI
                     LLVEKNNVPNSVKSELKRLNPKDVIIIGDENAVSKTTANQIKSTVNASQTRLNGSNRY
                     ETSLLIAKEIDKNHDVEKVYITNANGGEVDALTIAAKAGQDKQPIILTDKDSITDNTY
                     KWLKSEDLQNAYFIGGPQMISTNVINKVNDITKDNVTNNRVYGADRHETNANVIKKFY
                     TDDELEAVLVAKSDVLVDALAAGPLAANLKSPILITPKTYVSAYHKENLEAKSANKVY
                     KIGGGLTSKVMNSIASSLSKHNTTPTEPGNSGGKTVMIDPGHGGSDTGTTGKPLGGIK
                     EKDYTLNTSLATTEYLRSKGFNVIMTRDTDKTLSLGNRTALSNSLRPDLFTSIHYNAS
                     DTTGNGVEVFYKLKDKDGGTTKTVATNILNRILEKFNLKNRGAKTRTLSTDPTKDYLY
                     VLRNNDMPAVLVECAFLDNEKDMSLLNTSNKVKEMGTQIGKGIEDSLK"
BASE COUNT         1152 a          356 c          489 g          879 t
ORIGIN      
        1 attaagatat atgtgtagtt tttagagtgc taactctaaa aaattagttt tactaattta
       61 taaaaattta atgcgggtgt agctcaatgg tagagttctg gccttccaag ccagctgtga
      121 gggttcgatc cccttcaccc gctccaaaag attatgtggt tacaatagca gagaggatac
      181 acctgttccc attccgaaca cagaagttaa gctttctagc gctgatggta cttggtggga
      241 aactgcctgg gagagtagga cgtagccacg taatcttttt ttgttgtatt aaatttaata
      301 aacctacaat tattatatgt ttttatttgt cttttataga tttttttggc aataaatgat
      361 atagtgaaga taattaaata tattcaatat atttaatttt tttatttttt gtttttataa
      421 tttttttaaa agtttatttt ttatgtactt atttataatt ttgggtaaaa acactttgat
      481 atattttcaa aagttaaaag attaaaaaac aaagtagaaa tttatgttaa gtaaggagat
      541 taatatgaga agaaatacaa aattattaac aacagggatt ctttcaatgg caatcgtcac
      601 acctacaatg gcatttgcta ctgaatctaa tgctatggaa aataacgctg atttaaatat
      661 aaacttagag aaaaaaagta tagttttagg tagtacctca aaagtcagtg ttaagtttaa
      721 agaaaaacca gatgcagata gcattacatt aaagtataaa tgctatgata tgccattgga
      781 tacaactcta aattacaatc aatcaactga gtcatatgaa ggaactatca attataataa
      841 agacccagaa tatctaaatg tttgggaact acaagggata acaataaaca gcaaaaataa
      901 tcctaaaact ttaaataaac aagacctaga gaatatggga ttaaatttaa aagactataa
      961 tgtaacacag gaatgtataa ttgaagacat aacttctaga aaagatgtaa ataaatattt
     1021 gagaaaaact tctgcaccta ttacagaact tacaggaagt gatagatacg aaacagcagt
     1081 taaaataagt aaagagggct ggaaaaatgg ttcagataag gtagttataa taaatggaga
     1141 tgtaagtata gatggtatta tatcaactcc actggcaact acatataatg cgccaatact
     1201 tttggttgaa aaaaacaatg tgcctaatag tgtaaaatca gaattaaagc gcctaaatcc
     1261 taaagatgta attataattg gagatgagaa tgctgtttct aaaactactg ctaatcaaat
     1321 taaatcgact gtaaatgcta gtcaaacacg tctaaatggt tctaatagat atgagacatc
     1381 tttattgata gcaaaggaaa tagataaaaa tcatgatgtg gaaaaagtgt acataacaaa
     1441 tgctaatggt ggagaagtgg atgcacttac tatagcagca aaagcaggtc aagacaagca
     1501 accaattata ttaactgata aagatagtat tacagacaat acatataaat ggttaaagag
     1561 tgaggattta caaaatgctt attttatagg tggacctcaa atgatatcaa caaatgttat
     1621 aaataaggta aatgatataa ctaaagataa cgtgactaat aatagagtat atggagcaga
     1681 tagacacgaa acaaatgcaa acgtaataaa aaaattctat acagatgatg aattagaagc
     1741 tgttttagta gccaaatcag atgtacttgt tgatgcctta gcagcaggtc cattggctgc
     1801 caacttaaaa tctccaatac ttataacacc aaagacgtat gtatctgcat accataaaga
     1861 aaacttagaa gctaaatcgg ctaataaggt atataaaata ggaggaggat tgacttctaa
     1921 ggtaatgaac tctatagcat catcattatc taaacacaat acaactccaa cagaaccagg
     1981 aaatagtggg ggtaagacag ttatgattga cccaggacat ggtggttcgg acactggaac
     2041 aacaggtaaa ccattaggag gtattaaaga aaaagactat accttaaata cttcacttgc
     2101 aacaactgaa tatttacgtt caaagggatt caatgtaata atgacaagag atacagataa
     2161 gactttatct cttggaaata gaactgcttt atctaactct ttgagaccag atttatttac
     2221 gagtatacat tataatgcat ctgatacaac tgggaatggt gtagaagtat tttataagct
     2281 taaagataaa gatggaggaa ctactaagac tgtagctaca aatatattaa atagaatttt
     2341 agagaaattt aatctaaaaa atagaggtgc aaaaacaaga acactatcaa ctgaccctac
     2401 aaaagattat ttatatgttt taagaaataa tgatatgcca gctgtacttg tagaatgtgc
     2461 atttttggat aatgagaaag atatgagttt acttaataca tcaaataaag taaaagaaat
     2521 gggtacacaa ataggtaaag gcatagaaga ttcattaaaa tagtctacaa tgagagcata
     2581 gaaaaattta aaataaatta atctgcaatt ttaaatttaa actaaagcta taagataaga
     2641 atgtaattat aatagtaata aagactgtat atacattgtg catataagtg caaaatgagg
     2701 tatatacagt ctttttatga ttaattgtaa aagaatttat attatatgaa gaatttacat
     2761 atcttaagga tacagctaat tgtcaagagt tggagtattc atttgtaata cacaataaaa
     2821 gtttaattac tatattttat tgtgtgtttt tgtgtttttt gtggattttt atgtca
//
LOCUS       DAEPXF010000299         2785 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain HB27
            SAMN13376389-rid12479423.denovo.307, whole genome shotgun sequence.
ACCESSION   DAEPXF010000299 DAEPXF010000000
VERSION     DAEPXF010000299.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13376389
            Sequence Read Archive: SRR10518432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2785)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2785)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001069624.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001069624.1
            Genome Coverage       :: 315x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/15/2021 01:54:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,165
            CDSs (total)                      :: 4,076
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 89
            rRNAs                             :: 10, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 10 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 19 of 69
            Pseudo Genes (incomplete)         :: 44 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 10 of 69
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2785
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13376389-rid12479423.denovo.307"
                     /strain="HB27"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="China: Hebei"
                     /collection_date="2017"
     gene            <1..2356
                     /locus_tag="KRL67_003782"
     CDS             <1..2356
                     /locus_tag="KRL67_003782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HBH1524612.1"
                     /translation="DKVNLERYSCDINVGLNKNQLQSRIDDNLINAFENGKTKTYKQI
                     FKDNIFTLFNLINIVLVCLLVSVGSFKNTLFIFIAVINTIIGVIQEIRAKRLLDNLSV
                     IISNKVSVIREKEKKEIDVQELVLDDIMILKTGNQICADSKVLNGKLEVNESLVTGES
                     DIIIKNKGDFLYSGSFVVSGEAFVRVENIGKDNYANKITNDAKISKKHNSQLRNSLNA
                     ILKIVGIIIIPLGIILFAKQHFGNGDSIATSVVGTVAAVNGMIPEGLTLLTSIALAVG
                     TIKLASHKTLVQELYCVETLARVDTLCLDKTGTITEGKMQVDDIVMLEEVDINEIMGN
                     ICNSLKDDNATLNAIRDKYNVLDTYKAKNLIPFSSERKYSGVTFENKGTYLLGAFEFI
                     FKEKNKKLRIEVERYSKKGNRVIVLAHSDSYMEDNNIPEDIKPLAFILILDKIRDEAK
                     ETLEFFYNEGVDIKIISGDNPITVSEVARKAGLKTASNFIDATTLKNDSDIYEAVDKY
                     SVFGRVSPQQKKQMILALKKQNHTVAMTGDGVNDVLALKEADCSIAMASGSDVTKNVS
                     NLVLLDSNFASMPKVVMEGRKVINNIQRASSLFLVKTIFSFFLSLLTLFFFSRQYPFV
                     PIQLSLIGAVTVGIPSFFLALEANKSRISGNFLLNILKNALPGALCVTVNVIIVYNIV
                     EYLGYSSSVFSTMCAILTGVCGLLILRQQCLPFNKERLFLIVSMTIAFVIGILFLGGL
                     FSFVALSRELILITMALAILMPIMIKVMLFVLDEILETIVPDLN"
     gene            complement(2442..2759)
                     /locus_tag="KRL67_003783"
     CDS             complement(2442..2759)
                     /locus_tag="KRL67_003783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine/purine nucleoside phosphorylase"
                     /protein_id="HBH1524613.1"
                     /translation="MNEFKNVTAVKKANVYFDGKVSSRVIILPNGERKTLGLMLPGEY
                     TFSTREEEIMEMLAGSMDVKLPGSNEFVTYKEGQKFNVPSDSSFDLKVNEVVDYCCSY
                     IAD"
BASE COUNT         1028 a          325 c          464 g          968 t
ORIGIN      
        1 ggataaggtt aatttagaaa ggtatagttg tgatataaat gttggtttaa ataaaaatca
       61 acttcaaagt agaatagatg ataatttaat taatgcattt gaaaatggaa aaaccaaaac
      121 atataaacaa atttttaaag acaacatatt tacacttttt aatctcataa atattgtttt
      181 agtctgttta ttagtttcag ttggttcttt taaaaatacc ttatttatat ttattgctgt
      241 aattaatact attataggag taatacaaga gataagagct aaaagattat tggacaatct
      301 atctgttata atttcaaata aggtatctgt cataagagaa aaagagaaaa aagagattga
      361 tgtacaagaa ttagtattag atgatattat gattttaaaa acaggtaatc aaatctgtgc
      421 agattcaaaa gttttaaatg gaaaattaga ggtaaatgag tctttggtta caggtgaatc
      481 cgatattatt ataaaaaata aaggcgattt tttatattct ggtagttttg tagtttctgg
      541 agaagctttt gtaagagttg aaaatatagg aaaagataac tatgcaaata agataactaa
      601 tgatgcaaag atttctaaaa aacataattc tcaacttaga aattcattaa atgcaatatt
      661 aaaaattgta ggtataataa taattcctct gggaattata ttgtttgcaa agcagcattt
      721 tggaaatgga gattctatag ctacatctgt agtaggtaca gtggcagctg taaatggtat
      781 gataccagag ggattaacat tacttacgag cattgctcta gctgtaggaa ctataaaact
      841 tgctagtcat aaaactttag tgcaagaact gtactgcgta gagactcttg ctagagttga
      901 tacactatgt cttgataaga caggtacaat tacagaaggt aaaatgcaag ttgatgatat
      961 agtaatgttg gaagaagtgg atattaatga aataatgggg aatatatgta attctctcaa
     1021 agatgataat gcaacactaa atgcaataag agataagtat aatgttttag atacgtacaa
     1081 agctaagaat ttaataccat tttcttcaga aagaaaatac agtggggtta cctttgaaaa
     1141 taaaggtaca tatttattag gtgcatttga gtttatattt aaggaaaaaa ataaaaaact
     1201 tagaatagaa gttgaaagat attctaaaaa aggaaacaga gtaatagttt tagctcatag
     1261 tgattcttat atggaagata ataatattcc tgaagatata aagccactag cttttatatt
     1321 gatattggat aaaattagag atgaagctaa ggaaacttta gaattttttt acaatgaagg
     1381 tgtagatata aaaattatat ctggtgacaa tcctataaca gtaagtgagg tagctagaaa
     1441 ggcaggtctt aaaacggcta gtaattttat tgatgcaaca acacttaaaa atgatagtga
     1501 tatatacgaa gcagttgata aatacagtgt ttttggaagg gtaagcccac aacagaaaaa
     1561 acaaatgatt ttagcattaa aaaaacaaaa tcatacagta gctatgactg gagatggtgt
     1621 aaatgatgta ctagcactta aagaagcgga ttgtagcatt gccatggcat ctggaagtga
     1681 tgttactaag aatgtttcta atttagtctt attagattca aactttgcat caatgccaaa
     1741 ggttgttatg gagggaagaa aagtaattaa taacattcaa agagcatctt ctttgttttt
     1801 agtaaagaca attttttcat ttttcttgtc gttattaaca ttatttttct tcagtagaca
     1861 gtatccattt gttcctatac aattatcact cattggggct gtaacagtgg gtataccatc
     1921 attcttctta gctcttgaag caaacaaaag tagaatttca ggaaactttt tactcaatat
     1981 attaaaaaat gctttaccag gagcactatg tgtgacagta aatgtaatta ttgtttacaa
     2041 tatagttgaa tatttaggtt atagttcaag tgtattttcc acaatgtgtg ctatattaac
     2101 tggtgtatgt ggtcttttaa tattaagaca acaatgtttg ccatttaata aagaaagatt
     2161 atttttaatt gtaagtatga ctattgcatt tgtcattggg attctatttt taggtggatt
     2221 attctcattt gtagcattga gtagagaatt aattctcata actatggcat tagcaatatt
     2281 aatgccaatt atgataaaag ttatgttatt tgtcttggat gagatattag agacaattgt
     2341 tccagattta aattaatttt aaaatgatat aaataagagc agttttagtt gaactgctct
     2401 ttttagatgt gtttaatttt atgtaagaat atgtattttt attaatcagc tatatatgag
     2461 caacagtagt caacaacttc attaactttt aagtcaaaac ttgaatcgct aggaacatta
     2521 aatttttgac cttctttata agttacaaac tcattgcttc caggcagttt tacatccata
     2581 cttccagcaa gcatttccat tatttcttct tctctagtag aaaaagtata ttctcctggc
     2641 aacattaatc ctaaagtttt tctttctcca ttaggcagta ttattactct gctacttacc
     2701 ttaccatcaa aataaacgtt tgccttcttt actgcagtaa cattcttaaa ttcattcatt
     2761 agataaaacc tccaaaatta tttgt
//
LOCUS       DAEPXF010000300         2779 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain HB27
            SAMN13376389-rid12479423.denovo.308, whole genome shotgun sequence.
ACCESSION   DAEPXF010000300 DAEPXF010000000
VERSION     DAEPXF010000300.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13376389
            Sequence Read Archive: SRR10518432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2779)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2779)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001069624.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001069624.1
            Genome Coverage       :: 315x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/15/2021 01:54:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,165
            CDSs (total)                      :: 4,076
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 89
            rRNAs                             :: 10, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 10 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 19 of 69
            Pseudo Genes (incomplete)         :: 44 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 10 of 69
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2779
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13376389-rid12479423.denovo.308"
                     /strain="HB27"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="China: Hebei"
                     /collection_date="2017"
     gene            complement(499..1050)
                     /locus_tag="KRL67_003784"
     CDS             complement(499..1050)
                     /locus_tag="KRL67_003784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBH1524614.1"
                     /translation="MSINTIIAKNLNRLRNERNLSLGQLAELSGVSKVMLSQIEKGDS
                     NPTVNTIWKIASGLNVPYTAILEQPQNETFIVSKTDIDVQVSENKDYRLYCYYPNTPT
                     RNFELFQMELEEGHSYTSVGHSEKSQEYIMIIEGQLKLEVNDSIYQLRENDSICFSAE
                     SMHTYHNQGEKTLKAVIINYYPV"
     gene            1241..2203
                     /locus_tag="KRL67_003785"
     CDS             1241..2203
                     /locus_tag="KRL67_003785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase family protein"
                     /protein_id="HBH1524615.1"
                     /translation="MRVLNNVTEAIGNTHMLHLSKIADEFGVQGNIYAKMEYLNPGFS
                     KKDRVALQMITEAENAGLLVPGQAVVELTSGNTGTGLAIACACKGYKFIACMSKGNSM
                     ERARMMKALGAEVVLVEQMPNSVHGQVSGEDLALVEIEAQKIAKERNAFRADQFNLDA
                     CNHAHEKYTAEEMWEQLDGNIDVFVDFLGSGSTFAGCSKTLKKHNSSIKCYVVEPETA
                     AIYSGKEITDQGHKIQGGGYSMDLPLVDKKLIDGYIQVTDEEATDVARKLAKLEGVFA
                     GFSSGANVCAAIKLLKSSEKGKNIVLTLNDSGLKYLSTDLYEEL"
     gene            2495..>2779
                     /locus_tag="KRL67_003786"
     CDS             2495..>2779
                     /locus_tag="KRL67_003786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphoryl diester phosphodiesterase
                     membrane domain-containing protein"
                     /protein_id="HBH1524616.1"
                     /translation="MQKRNLRNFIFTGKNILSKLVFIIKYNFSTLILFELFHKGLALF
                     FIWPSIRYIFDILIKSLHIVYLNMDNFIKVLTNPISIVLIILSVIILAFYA"
BASE COUNT         1073 a          289 c          445 g          972 t
ORIGIN      
        1 tatgtgttat ttatattaaa tataaagtgc atatagtgat aaaacaaaag tttcatataa
       61 atgaagaaaa aaatataaaa tgatttattt aactacttaa aaataaacta aaagggataa
      121 taaaatagct tttaggtcat ggtaataaag gtatttaaaa agagtgcata agcactccaa
      181 ttgtgagaaa tacatacaca aatcattatt acaacaatga aaaatgtaca tataagagac
      241 gttttgtatt tgttttaagt gataatattt taatactaac taaataatta tcacaatagt
      301 aaaaaagaaa aaggagtatt ttgtactcca tttgtcaaaa atataaaact tttatataag
      361 cttattataa cataattgat aaaagtgtaa agatatataa tgaatttata aagaaaaata
      421 aaataaaaaa gataaatcct aattaaagga tttatctttt tgtatagaaa ataattatta
      481 tattattaaa tggcatggct atactggata ataatttatt attactgctt tcaatgtttt
      541 ttctccttga ttatggtatg tatgcattga ttcggctgaa aaacaaatag agtcattttc
      601 tctaagttga taaatggagt catttacttc taattttaat tgaccttcta taatcataat
      661 atattcttgt gatttttcag aatgaccaac agatgtataa ctatgacctt cttctaattc
      721 catttgaaat aattcgaagt ttctagttgg tgtattggga taataacaat aaagacgata
      781 atctttattc tctgaaactt gtacatctat atcagttttt gatacaataa aagtttcatt
      841 ttgaggttgt tctagtattg cagtatatgg aacattcagt ccacttgcaa ttttccaaat
      901 tgtgtttact gtggggtttg agtctccctt ttcaatctgt gaaagcatta ctttacttac
      961 accagacaat tcagcaagtt gacctagact taagttacgt tcattacgta atctatttag
     1021 atttttagca ataatagtat taatactcat aatagctcct taaaatgtaa attattgaca
     1081 aatataattt acgaatgtat aatttattta acgttattta taacttacaa atgtataata
     1141 taatgaacgt taaatacact gtacatccgt aatataaatt atactgcata aagagttgtt
     1201 tttcaacgga tgatagttga gagattaagg gggaattgta atgcgtgtac ttaataatgt
     1261 tacagaggct ataggcaata ctcatatgtt acatttatcc aaaatagcag atgaatttgg
     1321 agttcaaggt aatatttatg caaagatgga atacttaaat ccgggattta gtaaaaagga
     1381 ccgtgtagca ttacaaatga ttactgaagc agaaaatgca ggcttattag ttccaggtca
     1441 agcagttgtg gaattaacaa gtggtaatac tggaacagga ctagcaatag cttgtgcatg
     1501 taaaggttat aaatttatag cgtgtatgtc aaaaggaaat tctatggaaa gagcaagaat
     1561 gatgaaagct ttaggtgctg aagtagttct tgtagagcaa atgcctaatt cagttcatgg
     1621 acaggtatct ggtgaagatt tggcacttgt agaaattgag gctcaaaaaa tagcaaaaga
     1681 aagaaatgca tttagagcag accaatttaa tttagacgct tgtaatcatg cacatgaaaa
     1741 gtatacagca gaagaaatgt gggaacaatt ggatggaaat attgatgtat ttgtagattt
     1801 tcttggaagt ggtagtactt ttgcaggatg ttcaaaaaca cttaaaaaac ataattcttc
     1861 tatcaaatgt tatgttgttg aacctgaaac agcagcaatt tattcaggta aagaaataac
     1921 tgaccaaggg cataaaatac aaggtggagg atattctatg gatttgccat tagtagacaa
     1981 gaaacttatt gatggttata ttcaagttac agacgaggag gcaactgatg tagcaagaaa
     2041 attagctaaa ttagaaggtg tatttgcagg attttcttca ggggcaaatg tgtgtgcagc
     2101 aattaagttg ttaaagagca gtgaaaaagg aaaaaatata gttttaactc ttaatgatag
     2161 tggtttaaaa tatttaagta ctgatttata tgaagaatta taataatagc acaatagtta
     2221 tgcaatgttt ttaaattaag tatatagatt taaattataa aaagtatctc aatgtaattt
     2281 ttgaaagtta taaaggagat acttttttat tttgtaaata tggagtatcc tatttataag
     2341 tatgaagcta tatatattga tgattagcta tgtaataaaa aagagtatgt gtttttgcat
     2401 ttatacatta taacttggaa taatacatat taatgttcta aatgtattta aaatatatga
     2461 taaaattaga ataaatagat acaaggagat aaatatgcaa aagagaaatt taaggaattt
     2521 tatatttaca ggtaaaaata ttttaagtaa acttgttttt ataataaaat ataattttag
     2581 tacactaatt ttatttgaat tatttcataa aggattagcc ttatttttta tatggccatc
     2641 tattagatat atatttgata tattaataaa aagtttacat atagtctacc taaatatgga
     2701 taattttata aaagttttaa ccaatcccat atcaatagtt ctgataatac tatcagttat
     2761 aatactagct ttttatgca
//
LOCUS       DAEPXF010000301         2778 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain HB27
            SAMN13376389-rid12479423.denovo.309, whole genome shotgun sequence.
ACCESSION   DAEPXF010000301 DAEPXF010000000
VERSION     DAEPXF010000301.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13376389
            Sequence Read Archive: SRR10518432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2778)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2778)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001069624.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001069624.1
            Genome Coverage       :: 315x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/15/2021 01:54:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,165
            CDSs (total)                      :: 4,076
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 89
            rRNAs                             :: 10, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 10 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 19 of 69
            Pseudo Genes (incomplete)         :: 44 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 10 of 69
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2778
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13376389-rid12479423.denovo.309"
                     /strain="HB27"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="China: Hebei"
                     /collection_date="2017"
     gene            208..1029
                     /locus_tag="KRL67_003787"
     CDS             208..1029
                     /locus_tag="KRL67_003787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HBH1524617.1"
                     /translation="MGYKLIVTDMDGTLLGNNHKVTDENKTALQKVIKSGINVTLATG
                     RAFDSAKCNVDFLKEDMPIIACNGSLIREQNGNIIYSNKIDTRTCLNILDVLDKYDIY
                     YQCNSIDSMLSKKIEGREDRLSVFLGSETEVIVKDDLREEIFKKDILKFVIIEEKNPS
                     ILDEIRKELRKVQGIKITSSWPNNIEVMNEGVDKGNAVKILAEKMNIDREDIIAFGDN
                     YNDIEMIKFAGLGVAMGNAEEFIKQEADYVTDTNQDSGVAKAIYKFLELKESLSS"
     misc_binding    1108..1319
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            1406..2722
                     /gene="argH"
                     /locus_tag="KRL67_003788"
     CDS             1406..2722
                     /gene="argH"
                     /locus_tag="KRL67_003788"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012101354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HBH1524618.1"
                     /translation="MKLWGGRFRKAENQLMEEFNKSFGYDCVLYKKDIEGSVAHVHMQ
                     VKCGLLTEEEGKSITEGLKGILEDVENGKLALDGEYEDIHSFTEINLIQRIGDVGKKL
                     HTARSRNDQVAVDMRLYAKEKANDLVDLISEFKSTIKDVADKNPVMMPGYTHLQRAQV
                     VTFKHHLMAYYSMFDRDEKRIKNAIEILDESPLGCGALAGTTHDIDRSITCEQLGFKK
                     VVDNFMDGVSDRDYLLELMSDFSIIMMHLSRLSEELILWSSQEFGFVEIDDLYTTGSS
                     IMPQKKNPDGAELIRGKTGRVYGNLFGLFTVMKGIPLAYNKDMQEDKEGFFDSVHTLE
                     MCIQIMDRMIATLKVNEDKMKQAVKNGFLNATEVADYLVKNNVAFRDAHGIVGSIVIY
                     CEDNKKAIEDLTLEELHKFSDAFKEDIYDFIDYESILNKGIKKNLK"
BASE COUNT         1136 a          248 c          546 g          848 t
ORIGIN      
        1 agcattataa gagtataaat tgaaaatatt cagaaaaata ctacaagaaa cgatttattt
       61 tgctcttaaa ataagttaaa tgaatgttat taattttgta aattgaaata gtttaagtat
      121 aaagttatga tataatctaa taagggtaat tctttagatt atattaagga aatataggta
      181 tacaataaaa agttatggag ttggattatg ggatataaat taattgtaac agatatggat
      241 ggaactttat taggcaataa tcataaagtt acagatgaaa ataaaactgc actacaaaaa
      301 gttataaaaa gcggtattaa tgtaacttta gcaacaggaa gagccttcga ttcagctaaa
      361 tgcaatgtag attttcttaa agaagacatg ccaataatag cttgtaatgg ttcattaata
      421 agagagcaaa atggaaatat aatatattcg aataagattg atacaagaac atgtttaaac
      481 attttagatg tattagataa atacgatatt tactatcaat gtaatagtat agattctatg
      541 ctttcaaaaa aaatagaagg acgtgaagat agattaagtg tatttttagg ctctgaaaca
      601 gaagtaattg taaaagatga tttgagagaa gaaatcttta aaaaagatat actaaaattt
      661 gtaatcatag aagaaaagaa tccatctatc ttggatgaaa taagaaaaga attgagaaaa
      721 gttcaaggaa taaagataac ctcttcatgg ccaaacaata ttgaagtcat gaatgaaggt
      781 gttgataaag gtaatgcagt taaaatttta gctgaaaaaa tgaatataga tagagaagat
      841 ataattgcat ttggagacaa ttacaatgac atagaaatga taaagtttgc tggtcttggg
      901 gtagctatgg gaaatgcaga agaatttatt aaacaagaag ctgattatgt tacagataca
      961 aatcaagaca gtggagttgc aaaggcaatt tataaattcc tagagttaaa agaatcactt
     1021 agttcttaaa atagtatata ataggaaaaa ataggttgac tattttgtat atagtaatta
     1081 taatacaaat agataaaata taaaaattta gattcagtga aaaagagagt agatatagag
     1141 aaaatctcag cgaattgggg gatggtgtga gcctaatgat aacactttat attgaaaaga
     1201 actttggaga atactacttg aaatttagta tggtagtagg taatctcgcc ttaagagaat
     1261 agagtgggta aacattgttt accaactaga gtggtaacac ggaatatttc gtctcttggt
     1321 ctttttgacc aagagatttt ttattttcat taagttacaa gaattataat taaaatataa
     1381 aaaattaaaa tatgaggtga aagttatgaa attatggggc ggacgcttta gaaaagcgga
     1441 aaatcaactt atggaagaat ttaataaatc ttttggctat gattgtgtac tgtacaaaaa
     1501 agatatcgaa ggaagtgtag cacatgttca tatgcaagta aaatgtggac ttttaacaga
     1561 agaagaagga aagtctataa cagaaggttt aaaagggata cttgaagatg tagaaaatgg
     1621 aaagttagca ttggatggag agtatgaaga tatacatagt tttactgaga taaatttaat
     1681 tcaaagaatt ggagatgttg gcaagaagct tcatacagca cgtagtagaa atgaccaagt
     1741 tgcagttgat atgagattgt atgccaaaga aaaagcaaat gatttggtgg atttaatatc
     1801 tgaatttaaa tctactataa aagatgttgc tgacaaaaac ccggtgatga tgccaggtta
     1861 tactcatctt caaagagcac aagtagtgac atttaagcat cacttaatgg catattatag
     1921 tatgtttgat agagatgaga aaagaataaa gaatgctata gaaatattag atgaatcacc
     1981 tttaggctgt ggggctttag caggaactac acatgatata gacagaagta taacttgtga
     2041 acaattagga tttaaaaaag tagtagataa ttttatggat ggagtaagtg atagagatta
     2101 tttactagag ctaatgagtg atttttcaat tataatgatg cacttaagta gattaagtga
     2161 agagttgata ctttggagtt ctcaagaatt tggatttgtt gagatagatg acttatatac
     2221 tactggaagt agtataatgc ctcagaagaa aaatccagat ggtgcagagc ttataagagg
     2281 taaaactggc agagtatatg gaaacttatt tggtcttttt acagtaatga aaggaatacc
     2341 attagcatat aataaagaca tgcaagaaga taaagaaggt tttttcgata gtgtacatac
     2401 tcttgaaatg tgtatacaaa taatggatag aatgatagcc acactaaagg taaatgagga
     2461 taaaatgaaa caggcagtaa aaaatggttt tttaaatgca acagaagttg cggattattt
     2521 ggttaaaaat aatgttgctt ttagagatgc acatggtata gtaggaagta tcgttatata
     2581 ttgtgaagat aataaaaaag caatagaaga tttaacatta gaagaattac ataagttttc
     2641 agatgctttt aaggaagata tttatgattt tatcgactat gagagtatat taaataaggg
     2701 aataaaaaag aatttaaaat aatgtattgg atttctaaaa gctgtaagtt tgaatgttat
     2761 catttaaatt tacagctt
//
LOCUS       DAEPXF010000302         2776 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain HB27
            SAMN13376389-rid12479423.denovo.310, whole genome shotgun sequence.
ACCESSION   DAEPXF010000302 DAEPXF010000000
VERSION     DAEPXF010000302.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13376389
            Sequence Read Archive: SRR10518432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2776)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2776)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001069624.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001069624.1
            Genome Coverage       :: 315x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/15/2021 01:54:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,165
            CDSs (total)                      :: 4,076
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 89
            rRNAs                             :: 10, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 10 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 19 of 69
            Pseudo Genes (incomplete)         :: 44 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 10 of 69
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2776
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13376389-rid12479423.denovo.310"
                     /strain="HB27"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="China: Hebei"
                     /collection_date="2017"
     gene            315..2126
                     /locus_tag="KRL67_003789"
     CDS             315..2126
                     /locus_tag="KRL67_003789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional diguanylate
                     cyclase/phosphodiesterase"
                     /protein_id="HBH1524619.1"
                     /translation="MKKKYDFLIYAAIWLVIIILFIFSLYHSIEHIKIISHTSTIRSE
                     TQKVVKQELNNERNDDLIKRLDNILIKLRTGDGENGFQRCDNKEFQQKLNQMDSMWES
                     MKKEIIKVRNGASGDKLYKLSEEYSVLSNQIVFISEKHSNEKLYSFATALFIYLIFST
                     ISLLIWEYYNKKRFKRIFYTDNLTKIKNQVAFENRAIEILHNASNKEYVLLNIDIDNF
                     KYINDTHGYEYGDKVLIIVATALSKTFNIKETCARIGSDNFVILAKYRDSILEDIREM
                     LTNAIISELDMNVTQTISYCIGAYLVEIDNLGYKSINSIMDKANIAHKVSKTRGISST
                     VWYNENLLKQLQMENSIYNYMYKALENEEFHMYLQPKFQISSLNVVSAEALVRWFSPE
                     LGFLSPDEFIPLFEKSGFIIELDFYMLKKACSFVKKTFMKKNQYTYPIAVNFSRVTIY
                     QNSFYQRFLDTVKEYEIPFKYIEIEVTESAFNEISQPVISILEELKKLGFLISMDDFG
                     SGYSSLSLLCSLSINGLKLDKSLLKETFNREKVYSIIQCIIEMSHRIGMSVVCEGIET
                     KKDLEFLNTIKCDVGQGFYFSKPIEEKEFFNKYVTKK"
     gene            complement(2214..>2776)
                     /locus_tag="KRL67_003790"
     CDS             complement(2214..>2776)
                     /locus_tag="KRL67_003790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH1524620.1"
                     /translation="NQFERSYDIGIFKNEQSIYKFIEKIPFVKKDNSNFNYTNYYMEF
                     EDIPEYYEVNFNNYLYIITRFSFIPDGTEIFFTWDEIHCWDEKIATEKTFINGETLVD
                     SYNFPNNEVKDYIQKRESLYIETKKYYEEKGLKIERHALGSEDGEYFELTNDSILYLL
                     DIEAVEIREQSKNIGEFLYKYKKRFK"
BASE COUNT         1046 a          289 c          404 g         1037 t
ORIGIN      
        1 taaaaagaaa cataaatgaa ttatacaaat atattattgg ggggatattt tataagaaaa
       61 gaagtgatgc agtaaattaa ttgagcaatt taaaaatgat ttaatgatag ggttgatgaa
      121 tgttttaaaa aaaattttag aaaaacgtat attggtgttt gtgttggagg tgatgaattt
      181 gtagaattta tagagtataa atgattaaac tatttaagat aaatacatat cactgtaaag
      241 tacttataat cattttttat aagagattga ttatttgcaa attatataga tatattacaa
      301 atgaggtaac aataatgaag aaaaaatatg attttttaat ttatgctgct atttggctag
      361 ttattattat tctttttata ttttctctat atcatagtat agagcatatt aaaattataa
      421 gtcatactag tactattcgc agtgaaactc aaaaggttgt taagcaagaa ttgaataatg
      481 aaagaaatga tgacttgata aaacgactgg ataatattct gataaagcta agaacaggtg
      541 atggagaaaa tgggtttcaa agatgtgata ataaagagtt tcaacaaaaa ttaaatcaaa
      601 tggattctat gtgggaatct atgaagaaag aaattataaa agttagaaac ggtgcttcag
      661 gtgataaatt atataaattg agcgaagagt attctgtgtt gtctaatcaa attgttttta
      721 tatctgaaaa acactctaat gaaaaattgt attcttttgc cactgcacta tttatttatc
      781 ttatattctc aacaataagt cttttaattt gggaatatta taataaaaaa aggtttaaaa
      841 gaatatttta tactgataat ttaacaaaaa taaaaaatca agttgcattt gaaaacagag
      901 cgattgaaat tttacataat gcatctaaca aagagtatgt actactaaat attgatattg
      961 acaattttaa atacattaac gatacacatg gatatgaata tggagataaa gttctaataa
     1021 tagttgcaac tgcactttct aaaactttta atatcaaaga aacttgtgca agaattggtt
     1081 cagataattt tgtaatactt gcaaaatata gagattctat tttagaggat attcgggaaa
     1141 tgctaactaa tgctataatc tctgaattag atatgaatgt aactcaaact atatcttatt
     1201 gtataggagc atatttagtt gaaattgata atttaggtta taagtctatc aattctataa
     1261 tggataaggc aaatattgca cacaaggtaa gtaaaacaag aggaatttcc tctacagtat
     1321 ggtataatga aaatctgtta aaacagttac aaatggaaaa tagtatttat aattatatgt
     1381 ataaagcgct agaaaatgaa gaatttcata tgtatttgca acctaaattt caaatatctt
     1441 ctttaaatgt tgtaagcgca gaagctcttg taagatggtt ttctccagaa cttggttttt
     1501 tatcaccaga tgaatttatt ccattgtttg aaaaaagtgg atttattatt gaattggact
     1561 tttatatgtt aaaaaaagcg tgttcatttg tcaaaaaaac gtttatgaaa aaaaatcaat
     1621 atacatatcc gattgcagtt aatttttcaa gggttactat ttaccaaaat agtttttatc
     1681 aaaggttttt agatactgtt aaagaatatg aaattccttt taagtatata gagatagagg
     1741 taactgaaag tgcttttaat gaaatatccc aaccagttat ttcgatttta gaggagctta
     1801 aaaagttagg gtttttaatc tcgatggatg attttgggtc aggatactct tcattaagtc
     1861 ttttatgttc tctatctatt aatggattga agttagataa aagcttactt aaagaaacat
     1921 ttaaccggga gaaagtatat agtattattc agtgtattat tgaaatgtca catcgtatag
     1981 gcatgtctgt tgtatgtgaa ggtattgaaa ctaaaaaaga cttggaattt ttaaatacaa
     2041 ttaaatgtga tgttggacaa gggttttatt tctctaaacc gatagaagaa aaagaatttt
     2101 ttaacaaata tgtaactaaa aaataatatt attattttta ttagtcaata cattgagtca
     2161 ttttatatct cattaaatga ctcaatgtat tgttatgtat ttgaaaatat aaattattta
     2221 aaacgttttt tgtacttata tagaaattct cctatatttt ttgattgttc tcttatttca
     2281 actgcttcta tatctagtaa ataaagtata gaatcatttg taagttcaaa atattcacca
     2341 tcttctgaac cgagtgcatg tctctcaatt tttaatcctt tttcttcata atattttttt
     2401 gtttctatat ataatgattc tcgcttttgt atgtagtctt tcacttcatt atttggaaag
     2461 ttatatgagt ccaccaatgt ttcgccattt ataaaagttt tctcagtagc aattttttca
     2521 tcccaacaat ggatttcatc ccaagtaaaa aatatctcag ttccatctgg aatgaatgaa
     2581 aacctggtaa taatatataa ataattattg aaatttactt cataatattc aggaatatct
     2641 tcaaattcca tatagtaatt tgtataatta aaattactat tgtctttttt tacaaagggt
     2701 attttttcaa taaatttata tattgattgt tcatttttaa aaattcctat atcataactt
     2761 ctttcaaatt gattaa
//
LOCUS       DAEPXF010000303         2773 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain HB27
            SAMN13376389-rid12479423.denovo.311, whole genome shotgun sequence.
ACCESSION   DAEPXF010000303 DAEPXF010000000
VERSION     DAEPXF010000303.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13376389
            Sequence Read Archive: SRR10518432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2773)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2773)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001069624.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001069624.1
            Genome Coverage       :: 315x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/15/2021 01:54:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,165
            CDSs (total)                      :: 4,076
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 89
            rRNAs                             :: 10, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 10 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 19 of 69
            Pseudo Genes (incomplete)         :: 44 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 10 of 69
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2773
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13376389-rid12479423.denovo.311"
                     /strain="HB27"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="China: Hebei"
                     /collection_date="2017"
     gene            184..1107
                     /locus_tag="KRL67_003791"
     CDS             184..1107
                     /locus_tag="KRL67_003791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2520 domain-containing protein"
                     /protein_id="HBH1524621.1"
                     /translation="MGSGKVGTSLANYFLSKGHCVSGFYGRNQLSLLESTNLTKTKIY
                     SNLKDIIYENDILFITTSDDSIEIIDKKLSKFNLKHKYICHTSGSLKSSILFCSKKAG
                     ALIYSIHPIFAFSNKNTDIKKMKDICFSIEGDNEQDDLVVQNFIDGLGNHFFIRDKDT
                     SSTYHLANVLVSNLVLSLLDIGVSYFIKLGLSEDESLKAISPLVKKNIENILDNGFTK
                     ALTGPVSRGDITPIKKHLSVLDKKDEEIYKILSLNLLKIIALGNDNSLKCTKNITIEN
                     AIENLISKSEKYKEIYKLLGGKNDEKYHTNF"
     gene            1082..1909
                     /gene="panB"
                     /locus_tag="KRL67_003792"
     CDS             1082..1909
                     /gene="panB"
                     /locus_tag="KRL67_003792"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011721175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HBH1524622.1"
                     /translation="MKNTIQTFKNAKYEGKKLSMLTAYDYSIAKIMDECDINGILIGD
                     SLGMVIKGEENTLSVTIDEIIYHTKAVKNGVKNALIVSDMPFLSYHVSIEDAVKNAGR
                     LIKEGGAHAVKLEGGSNVIKQIENIVNAQIPVMGHLGLTPQSVNSFGGFKVQGNTSET
                     AKQLIEDAKLIEKAGAFSIVLEGVPAKIAEMVTNSISIPTIGIGAGINCDGQILVYQD
                     MLGMFGDFVPKFVKQYANIGDIMKDSIKNYILEVKTGAFPQEKHSFSINESELEKLY"
     gene            1928..>2773
                     /locus_tag="KRL67_003793"
     CDS             1928..>2773
                     /locus_tag="KRL67_003793"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HBH1524623.1"
                     /translation="MLVKEIKLLRNIIKDWRKDGYSIGLVTTMGFLHEGHQSLIKKAV
                     KENDKVVVSVFVNPTQFGPNEDFNSYPRDIDKDFKYCMDSGATVVFNPSPEEMYLKGN
                     CTTINVSGLTDFLCGAKRPVHFGGVCLVVSKFLNIVTPDKAYFGEKDAQQLAVIKRMV
                     KDLNIDTEIIGCPIIRENDGLAKSSRNTYLSEEERKSALILNKSLSLAKEELVKGNLN
                     PKNIKELIIAKINSEHLAKIDYVEIVDSETLQPVKQIEHSILVAIAVFIGKTRLIDNF
                     TFELNI"
BASE COUNT         1097 a          338 c          461 g          877 t
ORIGIN      
        1 atagaattaa ttgtcaattt tttatgaaaa tggtaatata tttatggatg gtatcttgtt
       61 ataagaacta tactttaaat gtttttaatt attttgtctt atttaactca tatcctcagg
      121 aatgagatta gaaattaaga tgttgtatca caaagtggag gtgatactat taaaataggc
      181 tttattggtt ctggaaaagt tggaacatct ttggcaaatt actttttatc aaaaggtcat
      241 tgtgtttcag gcttttatgg aaggaatcag ttatctttat tagaatcaac aaatttaaca
      301 aaaacaaaga tttacagcaa tttgaaagat ataatttatg aaaacgatat attatttatt
      361 acaacatcag atgattctat tgaaattata gacaaaaaac tttccaaatt taatttaaaa
      421 cataaataca tttgccacac aagtggctct ttaaaatcta gtatattgtt ctgttcaaaa
      481 aaagcaggtg cattaattta ttctatccat cctatatttg cattttccaa taaaaatacc
      541 gacataaaaa aaatgaaaga catttgtttt tccatagaag gtgataatga acaagatgat
      601 ttagttgttc aaaatttcat agatggtttg ggaaaccatt tttttataag agataaggat
      661 acttcatcca cttatcattt agctaatgtt ttagtttcaa atttagtatt atctttacta
      721 gatataggag taagttactt tattaaatta ggtttatctg aagatgaatc tttaaaagct
      781 atcagtcctt tagtaaaaaa aaatatagaa aatatacttg ataatggctt tactaaagct
      841 cttactggac ctgtttctcg tggagatata acaccaataa agaaacatct atccgtattg
      901 gataaaaaag atgaggagat ttacaaaata ctctccttaa acctactaaa aataattgcc
      961 ttaggaaatg acaattcatt aaaatgtaca aaaaatatta ctatagaaaa tgcaatcgaa
     1021 aatttaataa gtaaatctga aaaatacaag gaaatttaca aacttttagg aggaaaaaac
     1081 gatgaaaaat accatacaaa cttttaagaa tgctaaatat gaaggaaaaa aactatctat
     1141 gcttactgct tatgactact caattgcaaa aataatggat gagtgtgata taaatggtat
     1201 tttaatagga gattcattag gaatggtcat aaaaggtgaa gaaaacactc tatctgttac
     1261 tatagacgaa attatatatc atacaaaagc tgttaaaaat ggtgtaaaaa atgccttaat
     1321 cgtaagtgat atgccttttc tatcttacca tgtttctata gaagatgcag ttaaaaatgc
     1381 tggtagactt ataaaagaag gaggagctca tgctgtaaag ttagaaggtg gctcaaacgt
     1441 tataaaacaa atagaaaaca ttgtaaatgc tcagatacct gtaatggggc atttaggatt
     1501 aactcctcaa tcagtaaatt catttggtgg gtttaaggtt caaggaaata ccagtgaaac
     1561 agcaaaacaa cttattgaag atgctaagtt aatagaaaaa gcaggtgcat tctctattgt
     1621 ccttgaagga gttcctgcga aaatagctga gatggtaact aattctattt caattcctac
     1681 aataggtata ggggcaggaa ttaactgtga tggacaaatt ttagtttatc aagatatgtt
     1741 gggaatgttt ggagattttg ttcctaagtt tgtaaagcag tatgcaaata taggagatat
     1801 aatgaaagat tcaattaaaa attacatcct agaagttaag acaggtgcat ttccacaaga
     1861 aaaacacagc ttttcaataa atgaatctga gttagaaaaa ttatactaat attaaggacg
     1921 gtacaaaatg cttgtaaaag aaattaaatt actaagaaat ataatcaaag attggagaaa
     1981 agatggctat tctatagggc ttgttacaac aatgggattt ttacatgaag gacaccaaag
     2041 tcttataaaa aaagctgtaa aagaaaatga taaagtagta gtgagtgtat ttgtaaatcc
     2101 aactcaattt ggaccaaatg aagattttaa tagctatcca agagatatag ataaagattt
     2161 taaatattgt atggatagcg gtgcaacagt agtctttaac ccatcaccag aagaaatgta
     2221 tttaaaaggt aattgtacta ccatcaatgt ttctggactt acagattttc tttgtggagc
     2281 taaaagaccc gttcattttg gaggcgtctg cctagttgtt tcaaagtttc taaatatagt
     2341 aacacctgat aaagcctatt ttggagagaa ggatgcccaa caattggcag taattaaaag
     2401 gatggtcaag gatttaaaca tagatacaga aataataggc tgtcctataa ttcgtgaaaa
     2461 tgatggatta gcaaaaagtt caagaaatac atatctctct gaggaagagc gaaaatctgc
     2521 tctaatatta aataagagct taagtttagc aaaagaagaa ttagtaaaag gcaatctaaa
     2581 tccaaagaat ataaaagaat taataatagc taaaataaat tctgaacact tagcaaaaat
     2641 agactatgtt gaaattgtag atagtgaaac attacaacct gttaagcaaa tagaacattc
     2701 tatacttgtg gcaattgctg tgtttatagg aaaaaccaga ctaattgata attttacatt
     2761 tgagttaaat ata
//
LOCUS       DAEPXF010000304         2723 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain HB27
            SAMN13376389-rid12479423.denovo.312, whole genome shotgun sequence.
ACCESSION   DAEPXF010000304 DAEPXF010000000
VERSION     DAEPXF010000304.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13376389
            Sequence Read Archive: SRR10518432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2723)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2723)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001069624.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001069624.1
            Genome Coverage       :: 315x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/15/2021 01:54:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,165
            CDSs (total)                      :: 4,076
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 89
            rRNAs                             :: 10, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 10 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 19 of 69
            Pseudo Genes (incomplete)         :: 44 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 10 of 69
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2723
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13376389-rid12479423.denovo.312"
                     /strain="HB27"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="China: Hebei"
                     /collection_date="2017"
     gene            complement(<1..547)
                     /gene="mobB"
                     /locus_tag="KRL67_003794"
     CDS             complement(<1..547)
                     /gene="mobB"
                     /locus_tag="KRL67_003794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein B"
                     /protein_id="HBH1524624.1"
                     /translation="MLLLDSFMKGHNFMYNILSVVSYSGVGKTTLIEGIIKELNKKGY
                     RVGVIKHTCHDFDIDEEGKDTYKHRKSGARKVCIISDKRVAYIEELKEKNPLYKMIQL
                     YEDMDLIIIEGYKNYRFKRLEVTRKGKYEDILSNKSDLIGIVSDIEYKLNIEQFNLND
                     YKNISKYIESCIKNDILSISDF"
     gene            complement(531..1712)
                     /locus_tag="KRL67_003795"
     CDS             complement(531..1712)
                     /locus_tag="KRL67_003795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HBH1524625.1"
                     /translation="MIELEDAIKIIEKNVLPINRVKIVNLIDAVNKVVAEDIYSTIDS
                     PPFDRSPYDGYAYNANSENKNLKVIGTLYAGEVFNRVLNKNEAVKIMTGGKIPQGANC
                     VIKKEDVTVIGDMITLNVKLKEYDNYIFKGEDIKKGQLIINKNQNIGYDGIGVLASLG
                     ISKVTVYDDLKVGILNTGTELQDINETLENGKIYDSNRYTLYSRLLKLGIQADTICNC
                     SDDILELEKKIKNMLQSVDFLITTGGVSVGDKDLIPDVFKKIGATELFWKINIKPGGA
                     CYVGTLGEKLLFGLSGNPHAAIVVFDNVIVPVIEYLTYKKGIHHIKAIFKGEFNKLSN
                     KDRFVSGKLYTSEGKLYVELNDKKDAKARLSATLRSNCMIKIKKDETIKENQLVDVVI
                     R"
     gene            complement(1923..2657)
                     /locus_tag="KRL67_003796"
     CDS             complement(1923..2657)
                     /locus_tag="KRL67_003796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH1524626.1"
                     /translation="MKLKRSLVCVTILGIILVGCHKENTKEKNQVANKATQQKTMTKV
                     QNDVNEIMNKDYKYIIKNMGIPYNTFYYIKPKVLKESNTMQDINTSSYMTLVYPKYTG
                     NDELDGSALYVDINENKVVNVETNSFSSQGISVIDAESSIVIEKSDHEKSAVSLENFR
                     HIDLGEYVGVEDSRINEIVGDANYDLTAYNHEGSKVVKSYRLKEDNKILKKEVLTISI
                     VDNKIKSIKTIESNKIVKIIKGTLLE"
BASE COUNT          853 a          492 c          216 g         1162 t
ORIGIN      
        1 caaaatcact tatacttaaa atatcatttt ttatacaaga ctctatgtac tttgatatat
       61 ttttataatc atttaaatta aactgttcaa tattcaattt atattctata tcactgacaa
      121 tacctatcaa atcagactta ttagataaaa tatcttcata cttacctttt ctagttactt
      181 ccaatctttt aaatctataa tttttatatc cttctatgat aatcaagtcc atatcttcat
      241 ataattgaat cattttatac aatggattct tctcttttaa ttcctcaata tatgctactc
      301 gtttatctga tattatacat acttttctcg ccccagattt tctatgttta taagtatctt
      361 ttccttcctc atctatatca aaatcatgac aggtatgttt aataactcca acacgatacc
      421 cttttttatt taattctttt attatgcctt caatcaacgt agttttacct actcctgaat
      481 atgagacaac tgacaatata ttatacataa aattatgtcc tttcataaaa ctatctaata
      541 acaacatcca ctaattgatt ttcttttatt gtctcatcct ttttaatctt aatcatacaa
      601 ttacttctta gagttgcaga taatctagct tttgcatctt tcttatcatt tagctctaca
      661 tacaatttac cttcactcgt atatagtttt ccacttacaa atctatcttt atttgaaagt
      721 ttattaaatt ctcctttaaa tatagctttt atatgatgta ttcccttctt atatgttaaa
      781 tactcaataa caggaactat cacattatca aataccacta ttgctgcatg tggattacca
      841 gaaagaccaa ataatagctt ttctcccaat gtacctacat aacaagctcc accaggttta
      901 atgtttatct tccaaaataa ttctgttgcc cctatttttt tgaatacatc tggaattaaa
      961 tctttatctc caacagaaac accacctgta gtaattaaaa aatctacact ctgtaacata
     1021 ttctttatct ttttttctaa ctccaatata tcatcagagc aattgcatat tgtatctgct
     1081 tgtattccaa gttttaataa tcttgaatat aaggtatatc tattactatc gtatatctta
     1141 ccattctcta aagtctcatt tatatcttgt agttctgtac ctgtattcaa aattccaact
     1201 tttaaatcat cataaacagt tactttggat attccaagac ttgctaatac tccaatacca
     1261 tcatatccta tattctgatt tttattaata atcaattgcc ccttttttat atcctcaccc
     1321 ttaaatatgt aattatcgta ttcctttaat tttacattta aagttatcat atcacctata
     1381 accgttacat cttctttttt tattacacaa tttgcaccct gtggtatctt tccaccagtc
     1441 attatcttca ctgcttcatt tttatttaat accctattaa atacttctcc tgcatataaa
     1501 gtccctataa cctttaaatt tttattttca gaatttgcat tatatgcata accatcatag
     1561 ggtgacctat caaatggagg actatcaatt gttgaatata tatcttctgc aactacttta
     1621 tttactgcat ctataagatt tactatcttg accctgttta ttggtaaaac atttttttca
     1681 ataattttta tggcatcttc taattctatc attttaaata ctccccaata tattttttat
     1741 ttaagatatt tctatgtaat atattataga ttataaaagt tggaaatact tttatcttat
     1801 atttaattat aactaatagt tatctataag tgcagtttaa attttataaa tctaataaaa
     1861 aaactgttcc cagctagaaa tttccttcta tcttgaaaca gcatcatatg ctttagaata
     1921 ttttattcta ataaagttcc ttttattatt tttacaattt tattactttc aatagttttt
     1981 atagatttta ttttgttatc cacaatactt attgtaagta cctctttttt taatattttg
     2041 ttatcttctt tcaatctata gctcttaact actttactac cttcatgatt atatgctgtt
     2101 aaatcataat tcgcatctcc tacaatttca tttattctag aatcttcaac accaacatac
     2161 tctcccaagt caatatgtct aaaattttct agtgatacag ccgatttttc atggtcactt
     2221 ttttcaatca caatgctaga ttcagcatca atgactgata taccttgtga tgagaaagaa
     2281 ttggtttcaa cattgacaac tttattttcg ttaatatcaa catataaagc acttccatct
     2341 agttcatcat ttccagtata ttttggataa actaatgtca tataacttga tgtatttata
     2401 tcttgcattg tatttgattc ttttaaaact ttaggtttta tataataaaa tgtattataa
     2461 ggtataccca tattctttat aatgtactta taatctttat tcataatttc attaacatca
     2521 ttttgaactt ttgtcatagt tttttgctgt gttgctttat ttgctacttg atttttctct
     2581 ttagtatttt ctttatgaca tccaacaagt ataataccta aaatagttac acaaactaat
     2641 gaccttttta acttcataaa tcaatcctcc tttcttaaat tttatagtat ttatctatat
     2701 ctcaatttac agttatctct ttt
//
LOCUS       DAEPXF010000305         2693 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain HB27
            SAMN13376389-rid12479423.denovo.313, whole genome shotgun sequence.
ACCESSION   DAEPXF010000305 DAEPXF010000000
VERSION     DAEPXF010000305.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13376389
            Sequence Read Archive: SRR10518432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2693)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2693)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001069624.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001069624.1
            Genome Coverage       :: 315x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/15/2021 01:54:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,165
            CDSs (total)                      :: 4,076
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 89
            rRNAs                             :: 10, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 10 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 19 of 69
            Pseudo Genes (incomplete)         :: 44 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 10 of 69
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2693
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13376389-rid12479423.denovo.313"
                     /strain="HB27"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="China: Hebei"
                     /collection_date="2017"
     gene            287..1654
                     /locus_tag="KRL67_003797"
     CDS             287..1654
                     /locus_tag="KRL67_003797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase"
                     /protein_id="HBH1524627.1"
                     /translation="MGLKEFDFIEESIDMLRTMSPTLETISDEIEEYFENILDEKNQE
                     YINVTSRIKSESSLREKIIRNRYLKKYGEASNLIHNVSDLIGLRIECRFIEDENKIYR
                     LLRRYFNKTDDKINYYNKENKNIKLKLSERQPNKQKNGFEIYKIDGVFAYLDREVKFE
                     LQIKSLVNVFWSEIEHKIIYKNNTYLLADKFIKDMMDSIKNNLTMIDNQLLSIYKNFH
                     SGKSFNMKVSKKEIEKLFAKLVYDAFSEKMNKSIGFVVDFKKPCETILSYSFNKQDIS
                     DEVLGNFMLDEFARLNEIVNKDIDFNEQIEFEREPTFDDKFCKDLGNHFRSRLNTEFP
                     WNLFFRILFEIEPYNNTKDFENFVEFIKENVLLEENREQLLCQFEEDSKLIIEDIYDC
                     IFNSISEIDSVEILYNYNLEKINFTSSEIINCICREYECYGEYLEEKEKLMNTFKEKL
                     IQIFE"
     gene            1679..2287
                     /locus_tag="KRL67_003798"
     CDS             1679..2287
                     /locus_tag="KRL67_003798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase"
                     /protein_id="HBH1524628.1"
                     /translation="MRHFSNTYELSKIILEEAICIEKTGVILAGGKNSRMGRDKAFLE
                     LHEKLFIEIAMEAFKNFDELIIISNNEELYSKYNIKVYNDIVKDVGPIGGIYTALEYA
                     KYDIVTIACDMPYLNNQIVERIANKMDDKSVISVTNGKLQPLCSGYKKSIINKVSLCI
                     RENDLKLRSFIDKIDKSYIYFDEEDLFLNVNTVDEYEKLTKV"
     gene            2335..>2693
                     /locus_tag="KRL67_003799"
     CDS             2335..>2693
                     /locus_tag="KRL67_003799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HBH1524629.1"
                     /translation="MNLLKCDSWAVILNNSDKESKAYKILDELKRNMYKVVAIDEEKK
                     PIEGIDVYECLKDVPHNIDVVAIIDKQSKMDIILEEVELLDIQNMWFEKGSFSEMMIK
                     KTKDLKLNIEYNLSLYD"
BASE COUNT         1152 a          202 c          461 g          878 t
ORIGIN      
        1 aattttcata tagtaaatga caaagattta caataaatgt atattttctt actcattcct
       61 taaaattact catttagttt aagaactctc atttcatatg taaagttggc aaaaaaatta
      121 aaattaaaag taaaaaacta aataaactaa tgttaatttg aaatatatat gaaggttttt
      181 ggagaatact tacttatgat aggaaaaaat gttaaaataa tagtgtaatc aaatataaag
      241 gactgaaata attaacagtt attaaatatt aggagtgtaa aaatttatgg gattaaaaga
      301 gtttgatttt attgaagagt ctattgatat gttgaggaca atgtcaccaa cattagaaac
      361 tatatcagat gaaatagaag agtattttga aaatatcttg gatgaaaaaa atcaagaata
      421 tataaatgta acctctagaa taaaatcaga atcaagttta agagaaaaga taattagaaa
      481 tagatattta aaaaaatatg gagaagcaag taatcttatt cataatgttt ctgacttaat
      541 aggattaaga attgagtgta gatttattga ggatgaaaac aaaatatata gacttttgag
      601 aagatatttt aataaaacag atgacaaaat aaactattat aataaagaga ataagaatat
      661 taaattaaaa ctatctgaga ggcaacccaa taagcaaaaa aatggatttg aaatatataa
      721 aatagatgga gtttttgcgt atttagatag agaagtaaaa tttgaacttc aaattaaatc
      781 tcttgtaaat gtgttttgga gtgaaataga acataaaata atttataaaa acaatacata
      841 tttacttgct gataaattta taaaggatat gatggactct ataaagaata atcttactat
      901 gatagataat cagcttttaa gtatttataa aaactttcat tcagggaagt cttttaatat
      961 gaaagtaagt aaaaaagaaa ttgaaaaatt atttgcaaaa ctagtatatg acgctttttc
     1021 tgaaaagatg aataaaagta taggttttgt tgtagatttt aaaaaaccat gtgagactat
     1081 attgagctat tcttttaata aacaagatat aagtgatgaa gttttaggta attttatgct
     1141 tgatgagttt gcaagattaa atgaaattgt aaacaaggat atagatttta atgagcaaat
     1201 agaatttgag agagaaccga catttgatga taagttttgt aaagacctag gaaatcattt
     1261 tagaagtaga ttaaacacag aatttccatg gaacctattt tttagaatat tatttgaaat
     1321 agagccatat aacaatacca aagactttga aaactttgta gagtttataa aagaaaatgt
     1381 gctactagaa gaaaataggg aacaattgtt atgccaattt gaagaagata gcaagcttat
     1441 aattgaagat atatatgatt gtatatttaa tagtataagt gagattgata gtgttgaaat
     1501 tttatataac tataatcttg aaaaaataaa ctttacatca agtgaaatca taaattgtat
     1561 atgtagagaa tatgagtgtt atggtgagta cttagaagaa aaagaaaagc ttatgaatac
     1621 atttaaagaa aagcttattc aaatctttga gtaataggtc aatgctgaag tggcgctaat
     1681 gcgccatttt tcaaatacat atgaattaag taaaattatt ttggaggagg cgatttgtat
     1741 agagaaaaca ggtgtaattt tggcaggggg aaaaaattct agaatgggta gagataaagc
     1801 ctttttagag ttacatgaga agctttttat tgaaattgca atggaagcat ttaaaaactt
     1861 tgatgagttg ataataatat ctaacaatga agaattatat tctaaataca atattaaagt
     1921 ttataatgat atagtaaaag atgttggtcc aattggagga atatatactg ctcttgaata
     1981 tgcaaagtat gatatagtta caattgcatg tgatatgcca tatctaaata atcaaatagt
     2041 cgaaagaata gctaataaaa tggatgataa aagtgtgatt tcagtgacaa atggaaagtt
     2101 acaaccattg tgttctggat ataaaaagag tataataaat aaagtatctt tatgtataag
     2161 ggaaaatgat ttaaagctca gaagctttat agataaaata gataaaagtt atatatactt
     2221 tgatgaagaa gatttatttt taaatgtaaa cactgttgat gagtatgaaa aattaactaa
     2281 agtttaatta gctatggttg taaaatagag taggatttat atggaggaga gtttatgaat
     2341 ttacttaaat gcgattcttg ggcagtaata ttaaataata gcgataaaga aagtaaagca
     2401 tataaaatat tagatgaact taaaaggaat atgtacaaag ttgttgctat agatgaagag
     2461 aagaaaccta ttgaaggtat agatgtgtat gaatgtttaa aagatgttcc acacaatatt
     2521 gatgttgtag cgattattga taaacaatct aagatggata taattttaga agaagtagag
     2581 cttttggata tacaaaatat gtggtttgaa aaaggaagct ttagtgaaat gatgattaaa
     2641 aagactaaag atttaaaatt aaatatagaa tataatttaa gtttatatga tga
//
LOCUS       DAEPXF010000306         2691 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain HB27
            SAMN13376389-rid12479423.denovo.314, whole genome shotgun sequence.
ACCESSION   DAEPXF010000306 DAEPXF010000000
VERSION     DAEPXF010000306.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13376389
            Sequence Read Archive: SRR10518432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2691)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2691)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001069624.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001069624.1
            Genome Coverage       :: 315x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/15/2021 01:54:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,165
            CDSs (total)                      :: 4,076
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 89
            rRNAs                             :: 10, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 10 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 19 of 69
            Pseudo Genes (incomplete)         :: 44 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 10 of 69
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2691
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13376389-rid12479423.denovo.314"
                     /strain="HB27"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="China: Hebei"
                     /collection_date="2017"
     gene            298..2433
                     /gene="rnr"
                     /locus_tag="KRL67_003800"
     CDS             298..2433
                     /gene="rnr"
                     /locus_tag="KRL67_003800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HBH1524630.1"
                     /translation="MIPGLKESLLGLINESAYNPLKKEELAEIFDIHSSEMPMFYNFL
                     NELEEDGYICFTKKGKIVSPNQMGYFVGKFVSHKKGFGFVESDVEYTQDLFISSDSIN
                     GAMHNDRVMAEIVVPATEEKRAEGRIVKVIKREITRIVGTFQSSKTFGFVTPDNKKFT
                     KDIYIPKKHFSGAVDNDKVVCEITVWPQEDRKPEGKIIEILGQKGERGVEIDSIIREH
                     GLPEEFPKKVLQEAEAVAVEIPQEEIKRRRDLRDLNIFTIDGDDAKDLDDAISIEVLS
                     SGNFKLGVHIADVTHYVREKNKLDKEALKRATSVYLVDKVIPMLPKTLSNGVCSLNPF
                     EDKLTLSIFMEIDHKGNVVKHEICESVINSKARMTYTEVSDILEKDDEKLKKTFEHVV
                     EDFKNAEILARILMSRREKRGAIDFNFPEAKIILNGKGEVVDIKPYERRISNKIIEEF
                     MLISNETIAEHFYWMGIPFVYRIHETPSLEKMEELSKFISTFGYIIKGDKEEVHPKAL
                     QGIIEKIKGKKEEGAISTIMLRSLKQAKYSPECSGHFGLAAKFYCHFTSPIRRYPDLQ
                     IHRIIKESLNNKISGKRQEQLTTIVDYASVQSSEKERKAELAERDVHDFYKALYMKDK
                     VGQEFEGVVSSVTSFGIFVELDNTIEGLIRLANMNDDYYIYDESSYSVLGERTKKSYK
                     IGDLVKIKVESVNVDFKEIDFEIVEKIEE"
BASE COUNT         1158 a          259 c          500 g          774 t
ORIGIN      
        1 accaatccta tttgatggtg tataattagg tgataaagag taatttaagc aattatgata
       61 aagaataatt taagtaatta taatgatact tacaaataat ttataaaaca tcataatttg
      121 tttgaagtta gtatgacaaa acttacatta aatcaaaaat ataaataagt aattttaact
      181 tgtaataata agaagaaaat atagaaaatt atgtgataag taaaaaatgt tttgtctgaa
      241 gaaatataaa agacaatata aataataata taatgaaaag taaaacagga ggtaaaaatg
      301 ataccaggac ttaaagaaag tttgctagga ttaataaatg aaagtgcata taatcctcta
      361 aaaaaagaag aattagcaga aatatttgat atacattcat cagaaatgcc aatgttttat
      421 aattttttaa atgaattaga agaagatggt tatatctgct ttactaaaaa agggaaaata
      481 gtatctccca atcaaatggg atactttgtt ggaaaatttg tatcacacaa aaagggtttt
      541 ggatttgtag aatctgatgt tgaatataca caggatttat ttatctcaag tgacagtata
      601 aatggtgcta tgcataatga tagagttatg gcagaaatag tagtacctgc aactgaagaa
      661 aagagagctg aaggaagaat agtaaaagta ataaagagag aaataactag aatagtaggt
      721 actttccaat caagtaaaac ttttggattt gtaacaccag acaacaagaa atttactaaa
      781 gatatatata tacctaaaaa acattttagt ggagcagtag ataatgataa ggtagtatgc
      841 gaaattacag tatggccaca agaggataga aaaccagaag gcaaaattat agagatacta
      901 ggtcaaaaag gtgaaagagg cgtagaaata gattctataa taagagaaca tggtcttcca
      961 gaagaatttc caaagaaagt attgcaagaa gcagaagctg tagctgttga gattccacaa
     1021 gaagagataa aaagaagaag agatttaaga gatttaaaca tatttactat agatggagat
     1081 gacgcaaaag acttagatga tgccatatca atagaagtat tatctagtgg aaacttcaaa
     1141 ctaggagttc acatagctga tgttacacat tatgtaagag agaaaaataa acttgacaaa
     1201 gaagctttaa aaagagctac atcagtttat ttagtagata aagtaattcc aatgctacca
     1261 aagactcttt caaatggagt atgtagctta aatccatttg aagataagtt aactctttct
     1321 atatttatgg aaatagacca taaaggaaat gtggttaaac atgaaatatg tgaatctgta
     1381 ataaattcaa aagctagaat gacttataca gaagtatctg atatattaga aaaggatgat
     1441 gaaaaactta aaaagacttt tgaacatgtt gttgaagatt ttaaaaatgc tgaaatttta
     1501 gctaggattc ttatgtcaag aagggaaaaa agaggagcta tagacttcaa tttcccagag
     1561 gcaaagataa ttctaaatgg aaaaggcgaa gttgtcgata taaagccata tgaaagaaga
     1621 atatctaata agataataga agaatttatg cttataagta atgagacaat tgctgaacat
     1681 ttttactgga tgggaatacc gtttgtgtat agaatacatg agacaccatc tttggaaaaa
     1741 atggaagaat tgagtaagtt tatatctaca tttggatata taataaaagg tgataaagag
     1801 gaagtacatc caaaagcatt acaaggtata atagagaaga taaagggcaa aaaagaagaa
     1861 ggagctatca gtactataat gcttcgttct ttaaaacaag ctaagtactc accagaatgt
     1921 agtggacact ttggattagc agctaaattc tactgtcact ttacttcgcc aataagaaga
     1981 tatcctgacc ttcaaattca tagaataata aaagaatctt taaataataa aataagtgga
     2041 aaaagacaag aacagcttac tactatagtg gattatgcat ctgttcaatc atccgagaaa
     2101 gaaagaaagg cagagttagc tgaaagagat gtacatgatt tctataaggc tctttatatg
     2161 aaggataaag ttggtcaaga atttgagggt gttgtatcta gcgtaacttc atttggaata
     2221 tttgttgagt tagataatac tatagaagga ttgataagac ttgcaaatat gaatgatgat
     2281 tactatattt atgatgagag ttcttatagt gttttaggag aaagaactaa gaagtcatat
     2341 aagataggag accttgttaa gataaaggtt gaaagtgtta atgtagattt taaagaaatt
     2401 gactttgaga tagttgaaaa aatagaggag tagataaaac agctaaaata agagataaca
     2461 agttgtagta tttatgatat gtataaatat atagtgcaca atactctata aaataaatga
     2521 taaaaatagg taattgagaa atatctaatg gaaatagtta actgaaagat aaataattaa
     2581 gaaatattta ttgaaagtag ataattgaaa aataagtaat tgagaaatgt ttaatagaag
     2641 tggctaattg aaaataagta attaagaaat gcttaataga agtggctaat t
//
LOCUS       DAEPXF010000307         2686 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain HB27
            SAMN13376389-rid12479423.denovo.315, whole genome shotgun sequence.
ACCESSION   DAEPXF010000307 DAEPXF010000000
VERSION     DAEPXF010000307.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13376389
            Sequence Read Archive: SRR10518432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2686)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2686)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001069624.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001069624.1
            Genome Coverage       :: 315x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/15/2021 01:54:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,165
            CDSs (total)                      :: 4,076
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 89
            rRNAs                             :: 10, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 10 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 19 of 69
            Pseudo Genes (incomplete)         :: 44 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 10 of 69
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2686
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13376389-rid12479423.denovo.315"
                     /strain="HB27"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="China: Hebei"
                     /collection_date="2017"
     gene            complement(9..464)
                     /locus_tag="KRL67_003801"
     CDS             complement(9..464)
                     /locus_tag="KRL67_003801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH1524631.1"
                     /translation="MGLRDKFAQSFARSKTMSGPEKKANEIMGKLLLKKAILPIVLMF
                     VIIIAGAMLKINSWVTLGINLVIAVGAFFYIRNSSKKYQNFKPYVGNLISLEKKGKKE
                     YVAIIKQGKLPVKLQIAYGGEDLEHVKKNQMVQISYNPDAKIAILVNRQ"
     gene            complement(692..1111)
                     /locus_tag="KRL67_003802"
     CDS             complement(692..1111)
                     /locus_tag="KRL67_003802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBH1524632.1"
                     /translation="MDTLGERIVYLRKAKNLKQYELEEMLGCDNLSKFERNIRKPNYE
                     ILKSIAEIFNVSVDWLLNGDNLSHKSDLICDSSSNYPLNSINSNEIKLLNNFRKLNDY
                     DKAKIEGMIELKLHEYEKEKDLRKTEYNKNKDEKIDK"
     gene            1410..1619
                     /locus_tag="KRL67_003803"
     CDS             1410..1619
                     /locus_tag="KRL67_003803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBH1524633.1"
                     /translation="MDARKKWIPFLGVQVKQRLIELNMTQRELAKKIGVNENYLSAIL
                     NGRRTGKKYKSSIYQLLNIEYSEDD"
     gene            1651..1860
                     /locus_tag="KRL67_003804"
     CDS             1651..1860
                     /locus_tag="KRL67_003804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH1524634.1"
                     /translation="MCKLDSDGVIECCRAIDDFITALSNIKSLNMERLNTLTKYSSTC
                     SILLKEGNYEGCTIVYRKMLEELKT"
     gene            complement(2077..2475)
                     /locus_tag="KRL67_003805"
     CDS             complement(2077..2475)
                     /locus_tag="KRL67_003805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBH1524635.1"
                     /translation="MDTLGKRIAYLRNSKKLTQRKLMDILKFENLGKYETGDRKPNCD
                     ILMSIADYFNVTTDWLLYGKEKVNVNSSVKEDKEDYLHVTNDEMTILNLYRQLNERDK
                     IKIEGILELKISEYKDLKKHSSNNNEDKMV"
BASE COUNT          995 a          346 c          329 g         1016 t
ORIGIN      
        1 ttattaaatt attgtctatt aaccaaaata gctattttag catctggatt ataacttatc
       61 tgaaccattt gatttttctt aacatgttca aggtcttcac caccataagc tatttgtaac
      121 ttaactggta acttaccttg ttttataata gcaacgtact cttttttacc ttttttctct
      181 aaactaatca aattgccaac ataaggttta aagttctgat actttttact agaatttctt
      241 atgtagaaga aagcaccaac agcaataact aaatttatgc caagtgtaac ccaagaattg
      301 attttaagca tagctccagc gattattatc acgaacatta aaacgatagg taatatagct
      361 ttcttaagaa gcagtttacc cattatttca ttagcctttt tctcagggcc actcatagtt
      421 tttgatctag caaatgattg cgcgaatttg tctcttaagc ccattttatc ctcctaattt
      481 taataaatat ttagttataa taacgagata ttacttgaaa ctaaaaattt actacattta
      541 tattatgttt gacttttgta taaataatta cattcaagta aagcaaaata tactaattat
      601 tttatcataa aattataaaa aagaaaataa atgaaataaa aatattagaa caaagaaatg
      661 atgtaaaatc gtatcaaaag caacataaaa attatttatc tattttctca tctttatttt
      721 tgttatattc agtttttctt aaatctttct ctttttcata ttcatgaagt tttaattcaa
      781 tcataccttc tattttggct ttatcataat catttaactt tctaaagttg tttaaaagct
      841 ttatttcatt agagtttatg ctattaagtg gatagtttga ggaggaatcg caaattaaat
      901 ctgatttatg tgatagatta tctccattta atagccagtc tactgaaaca ttaaatatct
      961 cagctataga ttttaatatt tcataatttg gttttctaat gtttctctca aatttactta
     1021 agttgtcaca gcccaacatt tcttctagtt catattgttt aaggtttttt gcttttctca
     1081 aataaacaat tctttctcct aaagtatcca taaacactct ccattcaatt aatgtcaaaa
     1141 agacttttta agatgtaaat agtttcaaat taaaggtcaa aatgacataa aaaccattga
     1201 cttaaggtca aaatgacttt ataattaact taatgatacg aatttacatc ctaattttag
     1261 cacaaagtaa tcaaaaaatc ttatttagta ttaaataaat ttatatactt aatatgtgta
     1321 catattaaaa atatatacta aatagagggg gtgcgtaaac caaaataata taaaagtaaa
     1381 tataaatcac ttagaaagga agttgataaa tggatgctcg aaaaaaatgg ataccttttt
     1441 tgggagtgca agtcaagcaa agacttattg aattaaatat gactcaaagg gaattagcga
     1501 agaaaatagg tgttaatgaa aactatttgt cagctatttt aaatggaaga agaacaggta
     1561 aaaaatataa atcatcaatt tatcaattac ttaatataga atattcagaa gatgattaat
     1621 aaatagtata taaagtaggt gaatattctt gtgtgcaaat tggattcaga tggggttata
     1681 gagtgttgta gagcaattga tgattttatt acagcactca gtaatataaa aagcttaaat
     1741 atggaaagat taaatacttt aactaaatat tccagtacat gttcaatcct tcttaaagag
     1801 gggaattatg aaggatgtac aattgtgtat agaaagatgt tggaagaatt aaaaacatga
     1861 gtaatgcatt tcttaggaac ataaattata catggaaatg tattatattt ttcaaagtac
     1921 gtaaactaaa atatggataa gataatctaa atattataaa tgtgcttgaa attagactat
     1981 acttgttttt aaataatcca atatccatat tttagtaata tactacaaaa aaataaggtt
     2041 aatagatgat gtaaaatcgt atcaaattat gtatgtttaa accattttat cttcattatt
     2101 attagaggaa tgctttttta agtctttata ttcagatatc ttaagttcaa gtattccttc
     2161 tatttttatt ttatcacgtt cgtttagttg tctgtataga tttaatatcg tcatttcatc
     2221 attagtaaca tgtaggtaat cttctttatc ttcttttaca ctactattga catttacctt
     2281 ctctttacca tagagaagcc agtcagtcgt aacattgaaa taatcagcta ttgacattag
     2341 tatatcacaa ttaggttttc tatctcctgt ttcatacttg cctaagtttt caaattttaa
     2401 aatatccata agtttgcgct gagtaagttt tttggagttt ctcaaataag caattctttt
     2461 tcctaaagta tccacaaaat acactccttt ctttttatgg gtaatgtcta aatgacattc
     2521 aaagctaaaa atatataaaa ttataatata aaactactaa attaaagtct aaatgacatt
     2581 ttacttaaat taatatgctc ataatatgat tttaacatat tatagttgaa aatatatggt
     2641 ttattttgat ttgtatatat aacaataggt ttaattgtta taaaaa
//
LOCUS       DAEPXF010000308         2682 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain HB27
            SAMN13376389-rid12479423.denovo.316, whole genome shotgun sequence.
ACCESSION   DAEPXF010000308 DAEPXF010000000
VERSION     DAEPXF010000308.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13376389
            Sequence Read Archive: SRR10518432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2682)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2682)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001069624.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001069624.1
            Genome Coverage       :: 315x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/15/2021 01:54:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,165
            CDSs (total)                      :: 4,076
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 89
            rRNAs                             :: 10, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 10 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 19 of 69
            Pseudo Genes (incomplete)         :: 44 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 10 of 69
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2682
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13376389-rid12479423.denovo.316"
                     /strain="HB27"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="China: Hebei"
                     /collection_date="2017"
     gene            <1..513
                     /locus_tag="KRL67_003806"
     CDS             <1..513
                     /locus_tag="KRL67_003806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HBH1524636.1"
                     /translation="IAILGSTFLYNRLKYFKAQNLHDGLTNVYNRKSFDIMYEKYIEK
                     DDNFALVMIDIDNFKLINDNYGHKFGDVVIKGITNTICVMLEQDDKVFRYGGEEFSVL
                     IRNKSGEEVVDIIDNIRVAIANKKWSEDVTVTISAGVAHISGSKNVLEEADKNLYKAK
                     QLGRNKVVYK"
     gene            544..2544
                     /locus_tag="KRL67_003807"
     CDS             544..2544
                     /locus_tag="KRL67_003807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBH1524637.1"
                     /translation="MKQSIAIVTDKITKLSSFLEENIRLVLGNYIDINHYYLEVLKED
                     DKIQGDFVLVMNDDRLNSMRKHLVQDSKIIVIRRTLKENEIYGLFSIPNGTEVLVVND
                     TKETTLETISLFYKIGVKNLKFTPYIEGKIYKNIDIAITPGVKEKVPSYINKVIDLGN
                     RYIDISTFIEIINGLRIDVKEIRKNLMAYSESLISLDNGVKDNYRQLFLKIEEQDVIL
                     NLSKDGIIFTSADGIINTFNKEALKILGINENPQGKNIEDVISEDLNILLGDKEIIDE
                     VVFTNKKYINVNKKSIFSMGNKAGIYYNLQEITYIKKLEQNLTNKLREQGQIARYTFK
                     DIKTKNKNMVKCIELAKKISKSDLSVLIIGESGTGKELLSQSIHNASNRSNQPFIAVN
                     CAAVPDSLLESQLFGYEKGSFTGALREGKKGLFELANNGTIFLDEIGDMPPLLQTKLL
                     RVLQEKQVMPIGSHNVINIDVRVIAATNKNLPKMIREGKFREDLYYRLNVLPIDVPSL
                     RNRKEDIITLMNFFLKNNIRLSPEVITILEKYNWPGNIRELQNVASYVSLMCDSMVFR
                     DDLPPYIKVECANNYEDGFGYTRKEFEEILKILYERRESSSGVGRGFIIEKLIQEGIE
                     ISESKVRKVLSYLSECGYISSKSGRGGSQITSEGIKLYNNIN"
BASE COUNT         1130 a          245 c          475 g          832 t
ORIGIN      
        1 attgctatat tagggtctac ttttttatac aacagattga agtattttaa agcacaaaat
       61 ttacatgatg gtcttacaaa tgtttataat agaaaaagtt ttgatattat gtatgaaaag
      121 tacatagaga aagatgataa ttttgcactt gtgatgatag atattgataa tttcaagctc
      181 ataaatgata attatggaca taaatttggt gacgttgtta taaagggaat aacaaacacc
      241 atttgtgtta tgttagaaca agatgataaa gtctttaggt acggaggaga agaattttct
      301 gtattaataa gaaataaatc tggtgaggaa gtagtagata taattgacaa tataagggtt
      361 gcaattgcaa ataaaaagtg gagtgaagat gtgactgtga ctataagtgc aggtgtagct
      421 catatttcgg gtagtaaaaa tgtactagaa gaagctgata aaaatctata taaagcaaaa
      481 cagcttggta gaaataaagt agtatataag taatcgtatg ctatataaat aagaggtata
      541 aatatgaaac aatcaatagc tatagtaact gataaaataa ccaaattatc atctttctta
      601 gaagaaaata tacgcttggt tttaggtaat tatattgata taaaccacta ttatctggaa
      661 gttttaaaag aggatgataa aatacaaggt gattttgtac tagtaatgaa tgatgataga
      721 ttaaacagta tgagaaagca tttagtacaa gatagtaaga ttatagtgat aaggagaaca
      781 cttaaagaaa atgaaatata tggattattt tcaataccaa atggaactga agtattggtt
      841 gtaaatgata caaaagaaac taccttagaa acgataagct tattttacaa aataggagta
      901 aaaaatttaa agtttactcc atatattgaa ggaaaaattt ataaaaatat agacatagct
      961 ataacgcctg gagtaaagga aaaagtacca agttacataa ataaagtaat cgaccttggt
     1021 aatagatata ttgatatatc tacttttata gaaataataa atggtcttag aatagatgtt
     1081 aaagaaatac gtaaaaattt aatggcatat tctgagagtt taataagttt agataatgga
     1141 gttaaagata attataggca actgttttta aaaatagaag aacaagatgt tatattgaat
     1201 ctatcaaaag atggaataat atttacttca gcagatggta taataaacac atttaataaa
     1261 gaagctttaa aaattttagg tataaatgaa aatccacaag gcaaaaacat agaagatgtc
     1321 atttctgaag atttaaatat tttacttgga gacaaagaaa taattgatga agtggtattt
     1381 acaaataaaa aatatataaa tgtaaataaa aaaagtattt tttctatggg gaataaagct
     1441 gggatatatt ataacttaca agaaataacg tatataaaaa aattagaaca aaatcttact
     1501 aataaattaa gagaacaagg tcagatagca aggtatactt ttaaagatat aaaaactaaa
     1561 aataaaaata tggtaaagtg tatagaattg gctaaaaaaa tatctaaatc agacctttct
     1621 gtgttaataa taggagaaag tggtactggt aaagaattat tatcgcaatc aattcataat
     1681 gcttctaata gaagtaatca accgtttata gcagttaatt gtgcagcagt tccagatagt
     1741 ctattagaaa gtcaactgtt tggatatgaa aaaggctctt ttacaggagc tttaagagaa
     1801 ggaaaaaagg gtctatttga acttgctaac aatggaacta tatttctgga tgaaattgga
     1861 gatatgcctc cattgctaca gacaaaatta ctaagggtat tacaagaaaa gcaggttatg
     1921 cctataggtt cccataatgt tataaatata gatgttaggg tcatagcagc aacaaacaaa
     1981 aacttaccaa agatgattag agaaggaaaa tttagagaag acctgtatta tagactaaat
     2041 gtattgccaa tagatgtacc atcattaagg aatagaaaag aagatataat aacacttatg
     2101 aatttctttt tgaaaaataa tataagactt tcgccagaag ttataactat tttagaaaaa
     2161 tataattggc caggaaatat aagagagtta caaaatgttg cttcttatgt aagtctaatg
     2221 tgtgatagta tggtttttag agatgactta ccaccatata taaaggtaga atgtgctaac
     2281 aattatgaag atggatttgg ttatacaaga aaagaatttg aggaaatact gaagattcta
     2341 tatgaaagaa gagagtcatc ctctggagtt ggaagagggt ttataatcga aaagcttata
     2401 caggaaggga ttgagatttc tgaaagcaaa gttagaaagg tgctctctta tttaagtgaa
     2461 tgtggatata tatcatctaa atctggtaga ggtggaagtc aaataacctc agaggggata
     2521 aaactttata ataacataaa ttaagttgag tgtattgata ttgaaatcac atattttgaa
     2581 tttagatatt taaataataa taaactttta tatttataga aattaattta aattatgtca
     2641 gaaagaatat tgtaatttat aattaaatat taataggttt tt
//
LOCUS       DAEPXF010000309         2676 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain HB27
            SAMN13376389-rid12479423.denovo.317, whole genome shotgun sequence.
ACCESSION   DAEPXF010000309 DAEPXF010000000
VERSION     DAEPXF010000309.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13376389
            Sequence Read Archive: SRR10518432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2676)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2676)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001069624.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001069624.1
            Genome Coverage       :: 315x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/15/2021 01:54:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,165
            CDSs (total)                      :: 4,076
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 89
            rRNAs                             :: 10, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 10 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 19 of 69
            Pseudo Genes (incomplete)         :: 44 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 10 of 69
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2676
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13376389-rid12479423.denovo.317"
                     /strain="HB27"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="China: Hebei"
                     /collection_date="2017"
     gene            complement(209..1021)
                     /locus_tag="KRL67_003808"
     CDS             complement(209..1021)
                     /locus_tag="KRL67_003808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HBH1524638.1"
                     /translation="MFSIGMFSKINKITTKTLRYYEDIGLLKPEYVDEFTKYRYYTTE
                     QLPKLHQIITLKQMGLSLAEIKKAVENPEEVENILMNKEKEMLDVIKSEEYKIIKLRN
                     YLMNLKGEVNMNDVIIKSLPKVKVASMRKVLKGYDELFNLFPNVMAEEMMKTGCTCAV
                     PDYCFNIYHDGEYRETDMDVEMCQAINDLKEDTDILKFKELDEVENAVCILHNGPYST
                     LGKTYADAFKWIEDNKYKVIGNPRESYIDGIWNKENEEDWLTEVQIPVKRIL"
     gene            complement(1097..2515)
                     /locus_tag="KRL67_003809"
     CDS             complement(1097..2515)
                     /locus_tag="KRL67_003809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium/proton antiporter"
                     /protein_id="HBH1524639.1"
                     /translation="MTELMIISGLVLLICITSSKVLYKFGVPILLIFILLGMLFGSDG
                     MVGIYFDNYELTKQLCSIGLIFIMFFGGFGTNWNMAKPVAIPSILMSSLGVIITAGVT
                     GIFCHMVIGTSLLEGLLIGAIAASTDAASVFAVLRSQKLNLKGSLASLLEVESGSNDP
                     IAYMLTLILLTLMNNSGVELIVPMVIKQIAFGLAIGFILAKLSVYILRHSNFEIEGFY
                     TIFITAIAILSYAFSEYLGGNGYLSVYISGIIIGNSRIPLKKSLVHFFDGISWIMQIM
                     LFFMLGLLSFPSKLPNVFGIAMAISIFMIVIARPLATFIVLAKFKFSNKEKLFISWVG
                     LRGAASVVFAIYAVTQGVAIENDIFHIIFFIALLSVGIQGSLIPTVARKLDLVDDNTP
                     VLKTFNDYDGEISSKLIEVNVDEGNKWANKSIIDSNIPDDILIVMIKRKEQVLIPKGS
                     TIIKTGDILVLSGDRIEELIQL"
BASE COUNT          930 a          478 c          279 g          989 t
ORIGIN      
        1 aaatttatag atagagcatc tttagaaaac tattataata aaaaaataag aggctctccc
       61 aaaatagaga taagcctttt ttcataattg gaattataaa aatatacttg atttcatatg
      121 aaaaagagtg tctcataaac taaaaagttt actttgagac accacctttt ttattctttt
      181 ttattcttat ttatactttg tagacagatt ataatatgcg tttcactggt atctgaactt
      241 ctgtcaacca atcttcttca ttctctttat tccatatacc atctatataa gattctctag
      301 gatttccaat cactttatat ttattgtcct ctatccattt aaatgcatct gcgtaagttt
      361 ttcctaatgt tgaatatgga ccattgtgta atatacaaac tgcattttca acttcatcta
      421 attctttaaa ttttaatata tccgtatctt cctttaaatc atttatagct tgacacattt
      481 caacatccat atcagtctct ctatattcac catcatgata tatattaaaa caataatctg
      541 gaactgcaca tgtacaccct gttttcatca tttcttcagc cattacattt ggaaatagat
      601 tgaaaagttc atcatatccc tttaaaactt ttctcataga agcaacttta acttttggta
      661 aagacttaat aattacatca ttcatattga cttctccttt caaattcata aggtaattac
      721 gtagtttaat tattttatat tcttcacttt taattacatc caacatttct ttttctttat
      781 tcattaaaat attttcaact tcttcaggat tttctacagc tttttttatc tcagctaatg
      841 ataaacccat ttgttttaaa gttattatct gatgtaattt aggtaattgc tctgttgtat
      901 aatacctata ttttgtaaat tcatctacat attctggttt taacaaccct atatcttcat
      961 aataccttaa tgtctttgta gttattttat ttattttaga aaacattcct atagaaaaca
     1021 tctcatcacc tgcgcattag atttgattaa tttaagttta aactttcccc ttaggttaaa
     1081 gtcaatacaa aataaattat aattgtatta attcttcaat tctatctcca ctcaatacca
     1141 aaatatctcc tgtttttata atggttgaac ctttaggtat taaaacctgt tcctttcttt
     1201 ttatcataac tattaatata tcgtctggta tattagaatc tattatactc ttattagccc
     1261 atttattacc ctcatcaaca tttacttcga taagtttaga actaatttct ccatcataat
     1321 cattaaatgt ttttaataca ggtgtattat catcaactaa atccagcttt ctggctacag
     1381 ttggtatcag tgaaccttgt attcctactg aaagcaacgc tataaaaaat attatatgga
     1441 atatgtcatt ctctatggct actccttgag taacagcata aattgcaaaa actactgaag
     1501 ccgcacctct aaggcctacc cacgatatga ataatttttc tttatttgag aatttaaatt
     1561 ttgctagaac tataaatgtt gctaatggtc ttgcaataac tatcatgaat atagatatgg
     1621 ccattgctat accaaataca tttggtaact tagaagggaa agataacaat cctaacataa
     1681 agaataacat tatttgcata atccatgaaa ttccatcaaa gaaatgaacc aaactttttt
     1741 taagaggtat tctactattt cctataataa tacctgaaat gtaaacactt aaatatccat
     1801 ttcctccaag atactcgcta aatgcataag aaagtattgc aatagctgtt ataaatatag
     1861 tataaaatcc ttctatctcg aagtttgaat gccttaaaat ataaacagat aattttgcta
     1921 gtataaatcc tatagctaac ccaaaagcta tttgttttat taccattggt acaataagtt
     1981 ctaccccaga attattcatc aatgtcaaaa gaattagtgt caacatatac gctattgggt
     2041 cattacttcc actttcaact tctaataatg aggcaagtga accctttaag tttagttttt
     2101 gagaacgcaa caccgcaaac acagatgcag catctgttga agctgcaatt gccccaatta
     2161 ataaaccttc aagtaaacta gttcctatta ccatgtggca aaatatacct gtgacaccag
     2221 ccgtaattat tacacccagt gaagacatta gtatagaagg tatagcaaca ggtttagcca
     2281 tattccagtt tgttccaaac cctccaaaaa acattataaa tattagtcca atagaacata
     2341 attgttttgt taattcatag ttatcaaaat agattcctac cattccatca gacccaaata
     2401 acatccctag taagataaaa atcaataata ttggtactcc aaatttatac aataccttgc
     2461 tagaagttat acaaatcaat agtactaacc cacttataat cattaattct gtcatagcat
     2521 caactccatt tcatataaat tttgtagatt aatttacatc tacaatttta ttacttattc
     2581 aaatataaca tgacataaat tttataagtt atggtacata aagctcaaca aaaatttcac
     2641 aatacattaa aatgtgagtg ttatacttaa atttat
//
LOCUS       DAEPXF010000310         2663 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain HB27
            SAMN13376389-rid12479423.denovo.318, whole genome shotgun sequence.
ACCESSION   DAEPXF010000310 DAEPXF010000000
VERSION     DAEPXF010000310.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13376389
            Sequence Read Archive: SRR10518432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2663)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2663)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001069624.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001069624.1
            Genome Coverage       :: 315x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/15/2021 01:54:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,165
            CDSs (total)                      :: 4,076
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 89
            rRNAs                             :: 10, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 10 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 19 of 69
            Pseudo Genes (incomplete)         :: 44 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 10 of 69
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2663
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13376389-rid12479423.denovo.318"
                     /strain="HB27"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="China: Hebei"
                     /collection_date="2017"
     gene            60..1886
                     /locus_tag="KRL67_003810"
     CDS             60..1886
                     /locus_tag="KRL67_003810"
                     /inference="COORDINATES: protein motif:HMM:NF022062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIPR family protein"
                     /protein_id="HBH1524640.1"
                     /translation="MSKTTIISHPVIKSYIDNFIAEHEISKSKAKDQHTIFEQYINDL
                     VLSIYSNDPSVTYQDMDTDTAFGIDGIAIFVSDRVVTSIEDVNSIIDEIRKFEVSFFF
                     TQSKTSDKFNRQDISDFFTGVRRFFNFDRCEIPELYTHWEVAKYIYSKGARFKKQPSL
                     NMKFICLSPKEIDLGNIHMKSEIDLGIADLNDMSLFNEVKTPEFLGIKDIMSLHEKNT
                     SGLEVSITLTKQPVPYPKDIHNRIKNGYYGLIKLEEFISLLTDEVNNERILRKGIFND
                     NIRYYLGSSEKVEVNASMKKQLLGDENYLFGLLNNGVTIIADNIFLSSEDLTLTNYQI
                     VNGCQTSNVIFECLNSIDNKTNDIYIPIRLISTEDEDTKHAIIKATNSQTQLKPEQLV
                     ALSSIQKAIEQYYNTKTRSSTCPLYYERRTEQYRDENIAKTKIINIPFQIKATSALFF
                     DLPHEVSGQYGKVVKQTGDLLFKDNHLSFLNSYYVSGLAWYKVERFVLNNDDGKKYRR
                     ARWHIIMLLKYLCCPHDKINLSIDKQSEENSLIIERILLDDEKTNTILKEAIEIIELS
                     LSQKEDIEEILADRKTFERKDTTTIIIEYVKKYITEKNVVNV"
     gene            complement(2301..2471)
                     /locus_tag="KRL67_003811"
                     /pseudo
     CDS             complement(2301..2471)
                     /locus_tag="KRL67_003811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902377.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
BASE COUNT         1042 a          346 c          335 g          940 t
ORIGIN      
        1 cgatttatgc tataatttat aaaagtagaa tatagaaatc aattgaaagg atccttatta
       61 tgtctaaaac aacaattata tctcacccag taataaaatc ctatatagac aattttattg
      121 ctgaacatga gatatctaaa tctaaagcaa aagatcagca cactattttt gaacaatata
      181 ttaatgactt agttctatct atttattcta atgacccaag tgtaacttat caagatatgg
      241 atactgatac agcttttggt atagatggta tagctatatt tgtctcagat agagttgtaa
      301 caagtattga agatgttaat tctattatag atgaaatcag gaaatttgag gttagtttct
      361 tttttactca atcaaaaaca tctgacaaat ttaatcgcca agatatttct gattttttta
      421 caggtgttag acgttttttt aactttgata gatgtgaaat accagaacta tatacacatt
      481 gggaagtagc aaaatatatt tattcaaaag gtgcacgatt taaaaaacag cctagtttaa
      541 acatgaaatt catatgttta tctccaaaag aaatagattt aggtaatata catatgaaat
      601 ctgaaataga tttgggtatt gctgatttaa atgatatgag cttattcaac gaagtaaaaa
      661 cacctgagtt tttgggcatt aaagatataa tgtctctaca tgaaaaaaac acctctggat
      721 tagaggtatc aattacgtta actaagcaac ctgtaccata tccaaaggat attcataata
      781 gaatcaaaaa tggatattat ggactaatta aattagaaga gtttattagt ttattgactg
      841 atgaagttaa taatgaaagg attttgagaa aaggtatttt taatgacaat atacgttact
      901 atttaggatc ttctgaaaaa gtagaagtta atgcttccat gaaaaaacag ttattgggag
      961 acgaaaatta tttatttggc ttattaaata atggagtaac aattattgct gataatattt
     1021 ttttaagttc tgaagattta acattgacta attatcaaat agttaatggc tgccaaacaa
     1081 gcaatgttat ttttgaatgt ttaaattcta tagacaacaa gaccaatgat atttatattc
     1141 ctattagatt aatttctaca gaagatgaag atacgaaaca tgctattata aaagctacta
     1201 atagtcagac tcaattaaaa ccagagcaac tggtggctct atcttctatt caaaaagcta
     1261 tagaacaata ctataatact aaaacaagaa gttcaacttg ccctctttat tatgaacgta
     1321 gaacagaaca atacagagat gaaaatattg ctaaaacaaa aattattaat attccatttc
     1381 aaataaaagc aacatctgca ttattttttg atttacctca tgaagtctct ggacagtatg
     1441 gtaaagttgt aaaacagact ggggatttat tattcaagga taaccatcta tctttcttaa
     1501 attcttatta tgtaagtggt cttgcttggt ataaggttga aagatttgta cttaataatg
     1561 atgacggaaa aaaatataga cgtgctagat ggcatattat tatgctttta aaatatcttt
     1621 gctgtcctca tgataaaata aatctttcta ttgacaagca atctgaagaa aacagtttaa
     1681 taattgaaag aatcttactt gatgatgaaa aaacaaatac tatattaaaa gaagctatag
     1741 aaattattga actaagtctg agtcaaaaag aagatataga agaaattctt gctgacagaa
     1801 aaacttttga aagaaaagat actacaacta ttattattga gtatgtaaaa aaatacatta
     1861 ctgaaaaaaa tgttgttaat gtttgatttt gaagacgttt ctacaaaaag aaacgtcttt
     1921 tctgtgttta atcattatca aaaaacagaa tagcaattat caatgatatt ccattaataa
     1981 aattctaaac atacttatat taataattca aaatatgtcc atattcttaa aattgagtaa
     2041 agctaaaata aaatattttt aactctgata atttgtcacc aaataaaaac actaccataa
     2101 ataaacatca gcaattttta tcttatagct tttagttctc aagaaatttt aattggtatt
     2161 attttctata tttttttact atcaaatact aaggaaataa tcacttttct ttatataatt
     2221 ttctaccatc acatagcact aacattaaat caaaatgcca ctcttaattg tctcaaacta
     2281 aaaagatact gatagcttta ttagtcatca atatcttctt cgttcaaatt tttaaaatat
     2341 tcttctaatt tgttcaagtt tttactatct aaaacttcat catcatgtaa tgctgaaata
     2401 aaactcttaa acaaattgtt atgcataaaa ctaaagaaac tctttgtctc aacttttaaa
     2461 taatctttct ctagtcagta agtatatatt tttatacctt ctaaaaatac atccaaatca
     2521 tcaaactttc ttagcaatta ctttatcatt atataatgtc aaatcaaagc tctcaaataa
     2581 attggtattc atatagcaaa caaatacttg gtatcaattt tttaatatct atttaaatag
     2641 tattttttat tttgtcattt tat
//
LOCUS       DAEPXF010000311         2580 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain HB27
            SAMN13376389-rid12479423.denovo.319, whole genome shotgun sequence.
ACCESSION   DAEPXF010000311 DAEPXF010000000
VERSION     DAEPXF010000311.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13376389
            Sequence Read Archive: SRR10518432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2580)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2580)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001069624.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001069624.1
            Genome Coverage       :: 315x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/15/2021 01:54:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,165
            CDSs (total)                      :: 4,076
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 89
            rRNAs                             :: 10, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 10 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 19 of 69
            Pseudo Genes (incomplete)         :: 44 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 10 of 69
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2580
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13376389-rid12479423.denovo.319"
                     /strain="HB27"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="China: Hebei"
                     /collection_date="2017"
     gene            complement(204..959)
                     /locus_tag="KRL67_003812"
     CDS             complement(204..959)
                     /locus_tag="KRL67_003812"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HBH1524641.1"
                     /translation="MLKVIISGYNGTMGRVLAECVKKDNELELVCGVARSADEKGHHS
                     DTKLYKTMTDVTEKADVIIDFSHHETLEPILSYALKNNTPVVLATTGYDENDLHKIEE
                     ASKNIPILLSSNTSLGVNILIKLVKDASKMLEGFDIEIIEKHHNRKEDAPSGTATMIA
                     NAIKEVLPESENTHGRYGRDCKRKPNEIGIHSIRGGNIISDHDVIFAGDNEVLTLNHH
                     SQSDEVFANGSIIAAKFLIGKQSGLYKMSDILG"
     gene            1399..2388
                     /locus_tag="KRL67_003813"
     CDS             1399..2388
                     /locus_tag="KRL67_003813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HBH1524642.1"
                     /translation="MLKEEKTYQEKEAKVFNGVAALIMIVLFLVVSIGIMVFGIVKLD
                     SGFTGIGSLMLILGLIFIVVDFILFFGLKMINPKEAIVLVLFGNYYGTIKKEGYYWVN
                     PFCSAINPAASRINVSKSSSDEKVDISTNSRGKKVSLKTMTLNNEKQKVNDLLGNPII
                     IGVVVIWKVIDATKAVFNVDNYNTFLSIQCDSTIRNVSRLYPYDVSEDGDEKSLRGSS
                     QEIADRLKDELQSRVDIAGIEVSEVRITHLSYAPEIAAAMLQRQQAEAIIAARKKIVE
                     GAVSMVEMALTNLSENNVVTLDEERKAAMVSNLLVVLCGNKDAQPVVNSSSIY"
BASE COUNT          945 a          313 c          389 g          933 t
ORIGIN      
        1 ggtttatatg atgaaaggac tatgaaataa taaacaatat gattccatat agtaataaat
       61 aagataatct ttaagagaaa tgattatatt taaaaggtaa gcataaaatt tataaacttt
      121 tagaatagaa atctcaatca attagatatt caaattgatg aatatgaata cctaaataga
      181 ttgagattta ttatgtacaa aacttatcct aatatatctg acattttata aagtccagat
      241 tgcttaccaa ttaaaaattt agctgcaatt atagaaccgt ttgcaaatac ttcatcagat
      301 tgagaatgat ggtttaatgt aagaacctca ttatcacctg caaatataac atcatggtcg
      361 ctgattatat tgccacctct tatagagtgt attccaattt catttggttt tcttttacaa
      421 tctctaccat atctaccatg tgtattttca ctttcaggca agacttcttt aattgcatta
      481 gctatcatag tagcagttcc actaggggcg tcttcttttc tattgtgatg tttttctatg
      541 atttcaatat caaaaccttc taacatcttt gaagcatcct taactaattt tattaatata
      601 ttcactccaa gagatgtatt tgatgataat aatataggga tgttttttga tgcttcttct
      661 attttatgta aatcattttc gtcatatcca gtagtagcta atactacagg tgtattattt
      721 tttagagcat atgacaatat tggctctaaa gtctcatgat gtgaaaagtc tattatgaca
      781 tcagcttttt cagttacatc agtcattgtt ttgtaaagct ttgtatcact atgatgacct
      841 ttttcatcag cagacctagc tacaccacaa accaactcta attcattatc tttttttaca
      901 cactctgcta aaactcttcc cattgttcca ttatagccac taattataac tttcaacata
      961 aattcctcct atgaaattaa taaaaatttg ttatatttct ataagtttat tttatcacta
     1021 atataattta tataaaataa cattagtgat aataagctta caatttattt tacattaaaa
     1081 aaatttaagt ggatttctca aaatatagat aaacttcttt ttcattgttt aaatgataaa
     1141 aattatttga cttcatataa aaaagagtgt cccatgaact aaaaagttaa ttttgagata
     1201 ctatattcat atatcatata atttatttgg aaatcaatat taatacatta aaataactaa
     1261 atacatgtat gcataaagat gtatggaaaa tatataaaca tatagtatta atttcaaatt
     1321 caacaattta agtgttaaaa gtgttattat taaattagaa atatcttaac ttaatatact
     1381 gagatgggag tgatagatat gttaaaagaa gaaaaaactt atcaagagaa agaagcaaaa
     1441 gtattcaatg gagtagcggc tttgataatg attgtattat ttttagtagt ttcaataggt
     1501 atcatggttt ttgggatagt aaaacttgat agtggattta ctggaatagg ttcacttatg
     1561 ttaattttgg gacttatatt tatagtagta gattttatct tattctttgg gctaaaaatg
     1621 attaatccta aagaagctat agttctagtt ttgtttggta actattatgg aactataaaa
     1681 aaagaagggt actattgggt gaatccattt tgttcagcaa taaacccagc agcatcaaga
     1741 attaatgtat cgaaatcttc atctgatgaa aaggtagata tatcaactaa ttcaaggggc
     1801 aaaaaagtat ctttaaaaac aatgactctt aataatgaaa agcagaaagt caatgattta
     1861 ttgggaaatc caattataat aggagtggtt gtaatttgga aggttataga tgcaacaaag
     1921 gcagtattta atgtggataa ttataataca ttcttatcta ttcagtgtga ttctactatt
     1981 cgtaatgttt caagacttta tccatatgat gtatctgaag atggagatga aaaatctctt
     2041 cgtggtagta gtcaggaaat agcagataga ttaaaagatg aattacaatc aagagttgac
     2101 attgcaggaa ttgaagtgag tgaagtgagg attactcatc tttcttacgc accagagatt
     2161 gcagcagcaa tgttacaacg tcaacaagca gaggctataa tagcagcacg taaaaagatt
     2221 gttgaggggg ctgtaagtat ggtagagatg gcattgacta atttaagtga aaataatgtt
     2281 gtgactttgg atgaagaacg taaagcagct atggtaagta atctgttagt tgtgctttgt
     2341 ggcaataaag atgcacaacc agtagtaaac agcagttcta tttattaaat tatagaaaat
     2401 ttaatagttt attctattga tatatagaaa atctagcagt tcattttatt aaattataga
     2461 aagattaatg gttcacttta ttgaattata aaaagtttaa taatttattc tattgatata
     2521 tagaaaattt agtagttcta tttattaaat tataaaaagt ttaatagttt attacattga
//
LOCUS       DAEPXF010000312         2563 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain HB27
            SAMN13376389-rid12479423.denovo.320, whole genome shotgun sequence.
ACCESSION   DAEPXF010000312 DAEPXF010000000
VERSION     DAEPXF010000312.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13376389
            Sequence Read Archive: SRR10518432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2563)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2563)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001069624.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001069624.1
            Genome Coverage       :: 315x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/15/2021 01:54:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,165
            CDSs (total)                      :: 4,076
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 89
            rRNAs                             :: 10, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 10 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 19 of 69
            Pseudo Genes (incomplete)         :: 44 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 10 of 69
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2563
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13376389-rid12479423.denovo.320"
                     /strain="HB27"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="China: Hebei"
                     /collection_date="2017"
     gene            complement(210..1460)
                     /locus_tag="KRL67_003814"
     CDS             complement(210..1460)
                     /locus_tag="KRL67_003814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1015 domain-containing protein"
                     /protein_id="HBH1524643.1"
                     /translation="MATIKPFKAIRPNKYIVDKVAALPYDVMNSKEARRIAEGNPYSF
                     LHIDKSEIDLDENIDLYDEKVYLKARENLDEFRKQEILVKDDKECIYIYKQIMDGRAQ
                     VGIVACISVDESLNGTIKKHEYTRPEKEIDRTKHIKYCDANTGTILVTYKHQRVIDDI
                     INDFMDNNEPLYDFITDDKIEHTVWKIDNDDIIDNLVDKFEKLDYLYIADGHHRTASA
                     ENVAKEMRAKNPNYTGKEEFNYFIAMIAPDENLMVLDYNRVIKDLNGLSEEEFINKIK
                     ENFELEEIEGKEKYKPDKKGTFGMYLGDKWYKMKANKNLLEIEDPVDSLDISILQDYV
                     IDAILGIDNPRVDKRIDFIGGIRGLEELEKRVNEDMKVAFAMYPTSIEDLIRVADANK
                     IMPAKSTWFEPKVRCGLFLHEINE"
     gene            complement(1492..2433)
                     /locus_tag="KRL67_003815"
     CDS             complement(1492..2433)
                     /locus_tag="KRL67_003815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-3-phosphoglycerate dehydrogenase"
                     /protein_id="HBH1524644.1"
                     /translation="MYNILVTDGIEKEAARKLRELDFNVIEQFYEKDVLGDKLKDVDV
                     LVVRSATKVTKDVIDKALEGKKLKLIVRGGVGLDNIDVKYAQANGIKVMNTPNASSIS
                     VAELTIGQLFVLARFINTANVTMRDGKWEKKKYKGTEINGKTLGLIGFGRIAKEVAKR
                     AELLGMNVIYTDIMGEAQGFNNYKFCDMEEVLENADFLSLHIPFDKNKGAVITEKEIN
                     KMKKGAYLINCARGGLVDEKDLLKALDEGKLSAAAIDVYEQEPTLNLDLVNHPRVSPT
                     PHIGASTVEAQERIGDEIVNVIQDFFLDFNNLIGVAL"
BASE COUNT          763 a          491 c          241 g         1068 t
ORIGIN      
        1 ccttataaat atacaaatac aattatataa attttcattc tcaattataa catagaatat
       61 ggctatctat atcccaaaat tatccatttt tcaaaaaagg taactattat taagaatatc
      121 aacttatata atttggattc cttatacgat tttcaattaa aaaaacaagg gctaaaaagt
      181 gaattgtaca ctttttagcc ctttattaat tattcattta tttcatgtaa gaatagccca
      241 catcttacct taggttcaaa ccatgttgac ttagctggca ttatcttatt tgcatctgct
      301 actcttatta aatcttcaat actagtagga tacattgcaa atgctacttt catatcttca
      361 ttaactcttt tttctaattc ttcaagacct ctaattccac cgataaaatc aattctttta
      421 tctactctag gattgtcaat tcctaaaata gcatcaataa catagtcttg taatatagaa
      481 atatctaatg agtcaacagg gtcttctatt tctaataaat ttttatttgc tttcatttta
      541 taccacttat cacctaggta cattccgaaa gttccttttt tgtcaggttt atatttttct
      601 tttccttcta tttcttctag ttcaaaattt tcttttattt tatttataaa ttcttcctcg
      661 cttaaaccat ttaagtcttt gataacccta ttataatcca aaaccattaa attctcatct
      721 ggagcaatca ttgctatgaa gtaattaaat tcttcttttc cagtataatt tggattttta
      781 gctctcattt cttttgctac attttctgct gaagctgtcc tatgatgacc atctgcaatg
      841 tatagataat ctaatttttc aaatttatct actaggttat ctattatatc atcattatca
      901 atcttccata ctgtatgctc tatcttgtca tctgttatga aatcatataa aggttcatta
      961 ttatccataa agtcattgat tatatcatct atcacccttt gatgtttgta agttacaagt
     1021 atagttcccg tattagcatc acagtatttt atgtgtttag ttctatcaat ttccttttct
     1081 ggtcgagtgt actcatgttt ttttattgtt ccattcaagc tttcatcaac tgatatacat
     1141 gctactatac caacttgagc tctaccatcc atgatttgtt tatatatata tatacactct
     1201 ttgtcatctt ttactagaat ttcttgtttt cgaaactcat ctaaattttc tcgcgctttt
     1261 aaataaactt tttcatcata taaatctata ttttcatcta aatcaatttc tgatttatct
     1321 atatgcaaaa atgaatatgg attcccttct gcaattcttc tagcttcttt actattcata
     1381 acatcgtaag gaagtgctgc tactttatct actatatatt tatttggtct aatcgcctta
     1441 aatggtttaa ttgttgccat tactaatttc ctcctaatta tacaaaatat tttataatgc
     1501 tacccctatt agattgttga agtcaagaaa gaaatcttga atcacattta ctatttcgtc
     1561 tcctattctt tcttgagctt ctactgtaga tgccccaata tgaggtgtag gagaaactct
     1621 tggatgattt actaagtcta aatttaatgt aggttcttgt tcataaacat ctatagcagc
     1681 tgctgataat ttaccttcat ctaatgcttt tagtaaatct ttttcatcaa ctaaaccgcc
     1741 tcttgcacag tttattaaat aagccccttt cttcatctta tttatttctt tttcagttat
     1801 tactgccccc ttattcttat caaatggtat gtgtaaagat aagaaatctg cattttctaa
     1861 aacttcttcc atatcacaga atttataatt gttaaagcct tgcgcctcac ccattatgtc
     1921 agtatatata acattcatac ctaataattc tgctctcttt gcaacttctt ttgcaattct
     1981 tccaaaacct ataagtccaa gtgtctttcc attaatttct gtacctttat attttttctt
     2041 ctcccacttt ccatctctca tagttacatt tgcagtattt atgaatcttg caagaacaaa
     2101 taattgacct attgtaagct ctgcaacaga tatactgctt gcatttggag tattcataac
     2161 ttttatgcca ttagcttgag catatttaac atctatatta tccagtccta ctccacctct
     2221 tactatcaac ttcaactttt taccctctaa tgctttgtct attacatctt tagtaacttt
     2281 agtagctgaa cgtactacaa gtacatcaac atctttaagt ttatctccta aaacatcttt
     2341 ttcataaaac tgctctatca cattaaaatc taactctctc aattttctcg ctgcttcttt
     2401 ctctattcca tcagtaacta atatattata cataacaaac tcctccaatt tttattcact
     2461 taatttttcc aatttttatt ttttaaagta ttttcatcaa taaaaaactt tacttaattt
     2521 aactttctgt ttatataatt ttcaaaatat ttttttatta aag
//
LOCUS       DAEPXF010000313         2543 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain HB27
            SAMN13376389-rid12479423.denovo.321, whole genome shotgun sequence.
ACCESSION   DAEPXF010000313 DAEPXF010000000
VERSION     DAEPXF010000313.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13376389
            Sequence Read Archive: SRR10518432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2543)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2543)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001069624.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001069624.1
            Genome Coverage       :: 315x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/15/2021 01:54:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,165
            CDSs (total)                      :: 4,076
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 89
            rRNAs                             :: 10, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 10 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 19 of 69
            Pseudo Genes (incomplete)         :: 44 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 10 of 69
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2543
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13376389-rid12479423.denovo.321"
                     /strain="HB27"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="China: Hebei"
                     /collection_date="2017"
     gene            137..1642
                     /locus_tag="KRL67_003816"
     CDS             137..1642
                     /locus_tag="KRL67_003816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HBH1524645.1"
                     /translation="MKIQNTEWGYIEWKHTYDENNPKQAMNIYIAVTMPGKKHFNHVH
                     YGQEQMIYILEGEGLYIINGVWKPFYQGMIFYIESGSTHETINTGDREIKELIVSNNV
                     DDVGESEVIDINPNNYLKKTLINYSESTLNLYAAVESIRGQFIDPFKIPLIIYDDSWN
                     IVLKNPYFPLFCFEKCNPMKFPQNCDCMNQKSSNQFVCEYGITIYNIPILYKSNSIGV
                     IRGGYVLLSDLNLDTEHNNLYDIPEGAARSIKRLLKQISKNIINFCSFNDIRKDLQEK
                     EKTIARTYHYGEQLEMNLKVAQDMVTNLRINHHFLFNTLNSMASIALDDGSYDLYSAI
                     IDLSRMFRYTMRSDLRFVELESEILYIKNYLNLQKLRYGDALKVKYLIPEKLYNLSVP
                     FNFIQPIVENAFTHGFRDIDTEKRIEIIARLDSQYAIIEIHNNGTILDGNNIDKIKAG
                     IRSNNGHGLALIYTKFTSAYGNNFDMDIKSSDNEGTYIMIKIPIENYKEYV"
     gene            1756..2454
                     /locus_tag="KRL67_003817"
     CDS             1756..2454
                     /locus_tag="KRL67_003817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HBH1524646.1"
                     /translation="MRRALIIDDEEAVLKIITHFIEMKDMPIKIIGKATSGDEAVDKI
                     IGLEPDIVFIDIQMPIYNGLEVIEKTSHLSKKINFVVITAFDYFEYAQKALRLNVKDI
                     LLKPLDMKEFTKSVEKIIGYQYTNNDLLNKILEYINLNYSNDLKLNDCARLFLTNPSN
                     ISRIFKSNLNTTFISYLNHIRIEKSIELLENTTKSINEIAEIVGYNSLNNFYKNFKME
                     KGMTPKVYKLNTKN"
BASE COUNT         1052 a          248 c          390 g          853 t
ORIGIN      
        1 aataattaat ataaaaatgt gtacaaacta atgaatattg atatagtaag ctatcatatg
       61 aaagagtatg tacacatttt tgtttactaa ttttttacaa tatgaacata aataatttac
      121 aggaaaggaa tcttttatga aaattcaaaa tactgagtgg ggatatattg aatggaaaca
      181 tacttatgat gaaaataatc caaaacaagc tatgaatata tacattgcag taactatgcc
      241 tggtaagaaa cattttaatc atgttcatta tggtcaagag cagatgatat atattttaga
      301 aggagagggt ttatatatta taaatggagt ttggaagcca ttttatcaag ggatgatttt
      361 ttatatagaa tctggtagca cacatgaaac tataaataca ggcgatagag aaataaaaga
      421 acttattgtt tcaaataatg tagatgatgt aggagagagt gaagttatag atattaatcc
      481 taataattat ttaaagaaga ctttgataaa ttatagtgaa agcacattaa atttgtatgc
      541 agcggtagaa tcaattcgtg gtcaatttat agacccattt aaaatcccat taattatata
      601 tgatgattca tggaatatag ttttaaaaaa cccttatttt ccactttttt gttttgaaaa
      661 atgtaatcct atgaaatttc ctcaaaattg tgattgtatg aatcaaaaaa gtagcaatca
      721 atttgtatgt gaatatggga taactatata taatatacca atactgtata aaagtaatag
      781 cataggtgtt ataagaggag ggtatgtact attatctgat ttaaatttag atacagagca
      841 taataattta tatgatattc cagagggagc tgctagaagt ataaaacgcc tcttgaaaca
      901 aatatctaaa aatataataa atttttgctc ctttaatgat ataagaaagg atttacaaga
      961 aaaagagaag actatagcaa gaacatatca ttatggagag caacttgaaa tgaatttaaa
     1021 ggtagctcaa gatatggtta ctaatttaag aataaatcat cactttttat ttaatacatt
     1081 aaattcaatg gcaagcatag cactagatga tggtagttat gatttgtata gtgcaataat
     1141 agatttatca agaatgttta gatatacaat gaggtctgat ttaagatttg tagaattaga
     1201 atctgaaata ttatacatta aaaattattt gaatttacag aaattacgtt atggagatgc
     1261 acttaaagta aaatatctta ttccagagaa gttatataac ctttctgtac cttttaactt
     1321 tattcaacct attgtagaga atgcatttac acatggattt agagatattg acacagaaaa
     1381 acgaattgaa ataattgcaa gattagattc tcagtatgct attatagaga ttcataataa
     1441 tggaactatt ttagatggaa ataatataga caaaataaag gcaggcattc gtagtaataa
     1501 tggtcatggt ttggctttaa tatatacaaa attcacatct gcttatggaa ataattttga
     1561 tatggatata aagtcgagtg ataatgaagg aacttacata atgattaaaa ttcctattga
     1621 aaattataag gagtatgtat agaacttatg aaaaattaat aagttttata aatgttaaga
     1681 aatatattag atgtttttat gttgttttat atatcttact tttttatatt atcaagctaa
     1741 aagggaggta ttctcatgag aagagcacta ataatagatg atgaagaagc tgttctaaaa
     1801 ataataacac attttataga aatgaaagat atgccaataa aaattattgg taaagcaaca
     1861 tctggtgatg aagcagtaga taaaatcatt ggtttagagc cagacatagt ttttatagat
     1921 attcaaatgc caatatacaa cgggttagaa gtaatagaaa aaacatctca tttatctaaa
     1981 aaaataaact ttgtagtaat aactgcattt gactattttg aatatgctca aaaagctttg
     2041 agattaaatg taaaagatat actccttaaa ccattggaca tgaaagaatt tactaagtct
     2101 gtagaaaaaa ttattggata tcaatatacc aataatgatt tattaaataa aatactggaa
     2161 tatataaatc taaattatag caatgactta aagttaaatg attgtgctag attattttta
     2221 accaatccat ctaatatatc taggattttt aaaagtaatt tgaatacaac ttttatatca
     2281 tatctaaatc atataagaat agaaaaatct attgagttac ttgaaaatac tacaaaaagt
     2341 ataaatgaga ttgcagaaat agttggatat aatagcctaa ataactttta taaaaacttt
     2401 aagatggaaa aaggaatgac tccaaaagtt tataaactga atactaaaaa ttgaaattga
     2461 taaataaaaa gtcaatatag ataattgttt gagtgtgtct gaataagtat actttaatta
     2521 tgataaaaat taaagcgctt taa
//
LOCUS       DAEPXF010000314         2530 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain HB27
            SAMN13376389-rid12479423.denovo.322, whole genome shotgun sequence.
ACCESSION   DAEPXF010000314 DAEPXF010000000
VERSION     DAEPXF010000314.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13376389
            Sequence Read Archive: SRR10518432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2530)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2530)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001069624.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001069624.1
            Genome Coverage       :: 315x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/15/2021 01:54:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,165
            CDSs (total)                      :: 4,076
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 89
            rRNAs                             :: 10, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 10 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 19 of 69
            Pseudo Genes (incomplete)         :: 44 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 10 of 69
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2530
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13376389-rid12479423.denovo.322"
                     /strain="HB27"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="China: Hebei"
                     /collection_date="2017"
     gene            420..899
                     /locus_tag="KRL67_003818"
     CDS             420..899
                     /locus_tag="KRL67_003818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HBH1524647.1"
                     /translation="MDKTTFTNNILESEQTLYRVSKSILGNDQDCEDAVNNAILKAYE
                     KLDFLKEEQYFKTWLIRIVINECNSLRRKRLKSLSFEDIFKNKKIDEKDDYSDLYTAI
                     QSLPKKIKIPIVLYYIEGYSVDEVKEILDIPQGTVKSRLSRGRRLLKTKLENTEVII"
     gene            896..1675
                     /locus_tag="KRL67_003819"
     CDS             896..1675
                     /locus_tag="KRL67_003819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH1524648.1"
                     /translation="MNKNILNNSFPEIPESFHNSLSKTLNSLPEREENYKMINNKTHK
                     LPFGKGLVATLAITLILGTTALAVGQISSLVGKSSTKATYTSIPTTEQVKKDFNFTPN
                     IISEFSNGYKFKGAYTVNEKALDKEDNVLGELKALSFEYKNGNDEISLKTANNVLGNS
                     SENLKVVDTYKDINISYEAFAQKFVPEGYKMTEQDKVDEKSGKYTFSVGPNNESGKVE
                     INEFKFLTWKQDGVYYSFTVQDSNLSMNELVKMVHEVIDAK"
     gene            complement(1883..2383)
                     /locus_tag="KRL67_003820"
     CDS             complement(1883..2383)
                     /locus_tag="KRL67_003820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBH1524649.1"
                     /translation="MIEGIKFIKAEEKYIYSYWQTFDKIAKERKYLAMDEAFPFEETV
                     EFIKNTINKNLPQLFIIDLESDNCIGWCDVLPKTEKVGYLGMGILKEYREKGIGSSLL
                     KQIIDLSKEYGYEKIELDVLKSNSRAIHVYKSLGFVEVNTISSGFTWNDRPVKEEVIQ
                     MELTLI"
BASE COUNT         1004 a          346 c          307 g          873 t
ORIGIN      
        1 taagtttcat tgttttttct taaaagccta cttttagtca atatactgtt atctttactc
       61 attaattaaa gaacttattt taaatctata catgaagtaa ataatctaaa agcattattt
      121 tctaactact caacattaaa tacttactat tttctaatta ctcaatataa acacttacta
      181 taaaactaaa tatataaata gcttctaaaa tttaatatat ctacatggct ttatatatga
      241 cgggaaaaaa tactagaaaa aaatatatta tagaatattt gtatagaata ggcaaaactt
      301 tcaaacttaa atttattaaa taatcataaa aaaagtgaac catctatagt aaaattttat
      361 ctaataagtg aaaggcaaaa aagtagcgct acgatttgca tagaaaggaa ggaactcagt
      421 tggataaaac tacatttaca aacaatatac ttgaatcaga acagactttg tatcgagtat
      481 ctaagtctat tctaggaaac gaccaagact gtgaagatgc cgtcaacaat gccatcttaa
      541 aagcatatga aaagttggat tttcttaagg aagaacaata ctttaaaact tggttgatta
      601 gaatagtcat aaatgaatgt aattcattaa gacgaaaacg attaaaatca ttatcatttg
      661 aagacatctt caaaaacaaa aaaattgatg aaaaagatga ctacagcgat ttgtatactg
      721 ctattcaaag tctacctaaa aaaattaaaa ttccaatagt cctttactat attgaaggtt
      781 attcagtaga tgaagttaag gaaatattag atatacctca aggaactgta aaaagtagat
      841 tatcaagagg aagaagatta ttaaaaacta aattagaaaa cacggaggta atcatatgaa
      901 taaaaatata ttaaataatt cgtttccaga aattccagaa agttttcata atagtttaag
      961 taaaacacta aatagtttac cagaaaggga agaaaattat aaaatgatta ataataaaac
     1021 acataaatta ccatttggaa aaggcttagt tgcaactttg gccattactt taatattggg
     1081 cacaacagcc cttgcagtag gacagatatc cagtctagtt ggaaaatcaa gtacgaaagc
     1141 tacctataca tctatcccaa ctacagagca agtaaaaaaa gactttaact ttacccctaa
     1201 tataatttct gaatttagta atggatataa atttaaaggt gcatatactg taaatgaaaa
     1261 agcacttgac aaagaagata atgttttagg agaattaaaa gcacttagtt ttgagtataa
     1321 aaatggtaat gatgaaatat cacttaaaac agcaaataat gtacttggaa atagttctga
     1381 aaatctaaaa gttgtagata cttacaaaga tattaatatt tcttatgaag catttgcaca
     1441 aaagtttgta ccagaaggtt ataaaatgac agaacaggat aaagttgatg aaaaatcagg
     1501 caaatacaca tttagtgtag gtccaaataa tgaatcaggc aaagttgaaa ttaatgaatt
     1561 taagttttta acatggaaac aagatggagt ttactattct tttacagttc aagactcaaa
     1621 tttatctatg aatgaattag ttaaaatggt tcatgaagta atagatgcaa aataaacata
     1681 agtaataata agttgtttat gagtttatta tttacgactt taattttttt attagtaaac
     1741 atggttgtat cttccatctt tatattttct ttaaatttaa tatataaaat tttagatatt
     1801 taactacttt caaaagaacc actattaaaa agcccttaaa aatatttcat ttatatggaa
     1861 ttttaagggc tttaacttta ttttatataa gtgtaagctc catttgaatt acctcttctt
     1921 ttacaggtct atcattccat gtaaaaccac tagatattgt atttacctct acaaacccta
     1981 aggatttata aacatgaata gctctactat tacttttcaa cacatcaagc tcaatttttt
     2041 catacccata ttcttttgat aaatcaataa tttgttttaa aagactactt ccgatacctt
     2101 tttccctata ctctttaaga atacccatgc ctaaataacc aactttttca gttttaggca
     2161 aaacatcaca ccaaccaata cagttatctg attctaaatc aattataaac agttgtggta
     2221 agtttttatt tatagtattt tttataaatt caactgtttc ttcaaaagga aaggcttcat
     2281 ccatagctag atatttcctc tcttttgcta ttttatcaaa agtttgccaa tacgaataaa
     2341 tatacttttc ttctgccttt ataaatttta tgccttctat caacttaaca tttccccttt
     2401 ttaatttatg attgtagtta acttatttta tatgattcta gtttataatg ctatataaat
     2461 tactatatat tagcttaaaa tatctttgta tatttctata aaattaaaac caatatatct
     2521 atctcttgta
//
LOCUS       DAEPXF010000315         2530 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain HB27
            SAMN13376389-rid12479423.denovo.323, whole genome shotgun sequence.
ACCESSION   DAEPXF010000315 DAEPXF010000000
VERSION     DAEPXF010000315.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13376389
            Sequence Read Archive: SRR10518432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2530)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2530)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001069624.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001069624.1
            Genome Coverage       :: 315x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/15/2021 01:54:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,165
            CDSs (total)                      :: 4,076
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 89
            rRNAs                             :: 10, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 10 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 19 of 69
            Pseudo Genes (incomplete)         :: 44 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 10 of 69
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2530
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13376389-rid12479423.denovo.323"
                     /strain="HB27"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="China: Hebei"
                     /collection_date="2017"
     gene            103..1815
                     /gene="ptsP"
                     /locus_tag="KRL67_003821"
     CDS             103..1815
                     /gene="ptsP"
                     /locus_tag="KRL67_003821"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HBH1524650.1"
                     /translation="MAYKGIGASPGVALGKALVVEHSELVIEKKSIDNVEAEIAKLED
                     AVAVSKEELVKVKEKASEELGAEEAEIFEAHLLVLEDPELIGSAIDKIKTESVNAEYA
                     LNEIKEMFVSMFESMDNEYMKERAADIKDVTNRILRHVLGIKVVDLSALSEEVVLIAH
                     DLTPSDTATMNKKMVLGFLTDIGGRTSHTAIMSRTLEIAAIVGLNDITSKVKDGDFVV
                     FNGDTGEVIVNPDEETINKYTELKAKYEDERKALQLLKGKPSVTLDGKHVELAGNIGT
                     PNDIEGLIKNDAEGVGLYRTEFLYMDRDSFPTEEIQYEAYKAVLEGMDGKPIVIRTLD
                     IGGDKELSYLSMEPEMNPFLGYRAIRLCLDRKDIFKTQLRALYRASVHGRLRIMFPMI
                     SSLEELLQAKEVVKEVLAELDSEGVAYAKDVEIGMMIEVPSAAVISDVLAKHVDFFSI
                     GTNDLIQYTCAVDRMNQKISYLYNQFNPAVLRLIKTVIDNAHKEGKWAGMCGESAGDQ
                     KMIPILLGMGLDEFSMSPISILPARKLITSVKESDMKKLADDVLNMGTAEEIKSYIEK
                     TFNI"
BASE COUNT          999 a          242 c          473 g          816 t
ORIGIN      
        1 ttttataaaa aattataagt aaataaaact ataaaaatgt aaattattta tataaattta
       61 tagagtttat gtgctagatg atatttttta ggaggtaggg ttatggctta caaaggaata
      121 ggtgcttctc ctggtgttgc tttagggaaa gcgttagtag ttgaacatag tgaattagta
      181 attgaaaaaa aatctataga taatgttgaa gctgagattg ctaaattaga agatgcagtt
      241 gcagtttcta aagaagaatt agtaaaagtt aaggaaaagg cttctgaaga attaggagca
      301 gaggaagcag aaatatttga agcacattta ttagttttag aagacccaga attaattggt
      361 tctgcaatag ataaaattaa aactgaaagt gtaaatgcag agtatgcttt aaatgaaata
      421 aaagagatgt ttgttagcat gtttgagtct atggacaatg agtacatgaa agaaagagcg
      481 gcagatataa aagatgttac aaatagaata cttagacatg tattaggaat taaagttgtt
      541 gatttatctg ctttaagcga agaagttgta ttaatagcac atgatttaac tccatcagac
      601 acagcaacta tgaataaaaa aatggtttta ggatttttaa ctgatatagg tggaagaact
      661 tctcatactg ctatcatgtc tagaacttta gaaatagctg caatcgttgg cttaaatgat
      721 ataacttcaa aagttaagga tggagacttt gtagttttca atggggacac aggtgaagta
      781 atcgtgaatc ctgatgaaga gactataaat aaatatactg aattaaaagc taagtatgaa
      841 gatgagagaa aagcattaca gctattaaaa ggaaaaccat ctgtaacttt agatggaaaa
      901 catgtggaat tagctggtaa cataggaaca cctaatgata tagaaggtct aataaagaat
      961 gatgctgaag gtgttggtct ttatagaaca gaatttttat acatggatag agattctttc
     1021 ccaacagaag aaatacaata tgaagcatac aaagctgtat tagaaggaat ggatggaaag
     1081 cctatagtta taagaacatt ggatataggt ggagataaag aattaagtta tttaagtatg
     1141 gagcctgaaa tgaatccatt cttaggttac agagcaataa gactttgcct agatagaaaa
     1201 gatatattta agactcaatt gagagcttta tatagagcta gtgttcatgg aagattaaga
     1261 atcatgtttc caatgatttc atctttagaa gagttgttac aagctaaaga agttgtaaaa
     1321 gaagttttag cagaacttga ttctgaaggt gtggcttatg ctaaagatgt agaaattggt
     1381 atgatgatag aagttccatc tgctgccgta atatctgatg tattggctaa acatgtagat
     1441 ttcttctcta taggaactaa tgatttaatt caatacactt gtgcagttga tagaatgaat
     1501 caaaagataa gttatcttta caatcaattt aatccagctg ttttaagact tataaagact
     1561 gttatagaca atgcacataa agaaggaaaa tgggcaggta tgtgtggaga atctgctgga
     1621 gaccaaaaaa tgataccaat acttttaggt atgggattag acgagttctc tatgtctcca
     1681 atatcaatac ttcctgctag aaagttaata acttcagtta aagagtcaga catgaaaaaa
     1741 ttagctgatg atgtacttaa tatgggtact gctgaagaaa taaaaagcta tatagaaaag
     1801 acttttaata tataattaat gttgtatagt tactaaaatt cattttttta aataacaaaa
     1861 ttatatttta cgaattaaaa aggagatgtt tcaaaataaa aatttgaagc atctcttttt
     1921 tgtaaaagat taataaaaat tacttaattt gtgtaatagt cgttttgtga aaaagcaaaa
     1981 ctgcattgtg gctatagtag gtatggatgt tattagcaat aaaagtacta ttaaaatgaa
     2041 gattcattct caaatggtat caaaagtagt tgtgatggag caacataata atcttgtgat
     2101 ttaataatga aaattaaaaa aatatagtat tgaaaaataa ttaaaagtat ttaataatag
     2161 aattagggaa gaataagttt ataagtaaaa ataattgatt agtagttaat aaaataaatg
     2221 tgatatccaa ataaatccta aatctaattt gagttttaat atagatagtc taaagtttga
     2281 ttattaaaga tttaattgta agttgttaca gttgatgata ttgattagag attaaagttt
     2341 agagtagaaa gttaccttaa atttaaaaat gcaagataac ccaatctaaa aattataatc
     2401 actcgatagg tttaaggatt tatttgttta ttataaatag gagggtgtct caaaatgaac
     2461 tttttagttt atgaggcact ctcttttata tagaattaaa tatattttat tatttcaaca
     2521 atgaaaaaga
//
LOCUS       DAEPXF010000316         2505 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain HB27
            SAMN13376389-rid12479423.denovo.324, whole genome shotgun sequence.
ACCESSION   DAEPXF010000316 DAEPXF010000000
VERSION     DAEPXF010000316.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13376389
            Sequence Read Archive: SRR10518432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2505)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2505)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001069624.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001069624.1
            Genome Coverage       :: 315x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/15/2021 01:54:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,165
            CDSs (total)                      :: 4,076
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 89
            rRNAs                             :: 10, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 10 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 19 of 69
            Pseudo Genes (incomplete)         :: 44 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 10 of 69
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2505
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13376389-rid12479423.denovo.324"
                     /strain="HB27"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="China: Hebei"
                     /collection_date="2017"
     gene            complement(<1..204)
                     /locus_tag="KRL67_003822"
     CDS             complement(<1..204)
                     /locus_tag="KRL67_003822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1294 domain-containing protein"
                     /protein_id="HBH1524651.1"
                     /translation="MKNFTLIYFIVINSIAFFSMYIDKKRAVRNEWRIKEATLMSIAV
                     IGGSIGSMIGMYSFRHKTKHIKFT"
     gene            431..1132
                     /locus_tag="KRL67_003823"
     CDS             431..1132
                     /locus_tag="KRL67_003823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH1524652.1"
                     /translation="MKFIVEKEVFDKLENVCFGVVVAKGIDNTKEIERINNLLDISID
                     RVEDYFKDKKVKESEEIVPYREAFRSLGMNPNKFMSSIEAMTTRVAKNKKLPHINPIV
                     DLGNSISLKYLLPMGAHDMDFRNDDVYVRFSKKGDKFVPFGETDAELMEEGELIYSVG
                     DMVKTRRWIWRQGEEGKITNSSKNIFFPIDGFTDANLDKVMSAREELAKLLKEIFNCE
                     IKVGFVDKDNPEMEI"
     gene            1214..1822
                     /locus_tag="KRL67_003824"
     CDS             1214..1822
                     /locus_tag="KRL67_003824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HBH1524653.1"
                     /translation="MKKTIIKNVNPMIENKFIGLFEIEYKNKLDEDKVWMVASRKSNE
                     QLKNIYLKNEEDSVDAVVIVGLHKSSKKLILIRQFRVPINGYIYELPAGLVDEGESID
                     ISVERELREETGLTLLEIKKNKSIDKVYLSPGMSDESVAFVYCICDGDITDEFLEPDE
                     EIEALLISQEDAKEILQSNHKIDTKAFLILQMFVSLGTKLFE"
     gene            2146..2238
                     /locus_tag="KRL67_003825"
                     /pseudo
     CDS             2146..2238
                     /locus_tag="KRL67_003825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439554.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4624 domain-containing lipoprotein"
BASE COUNT         1037 a          211 c          424 g          833 t
ORIGIN      
        1 tgtaaattta atgtgtttag ttttatgtct aaaagaatac attccaatca tagaacctat
       61 actacctcca attactgcta tagacataag agtagcttct tttattctcc attcgtttct
      121 aactgctctt ttcttatcta tatacattga aaagaaagca atagaattaa tcactataaa
      181 atagattaaa gtaaaatttt tcacagcctt gcctccataa aaatatatta tacattataa
      241 aacaagatat ttgttttgga aagttaaatt tgtaattagt acatataaaa tatttgataa
      301 gaatcaatgt aatattaatt ggaaaattta aaaaaattaa acaattgcta aatagggggt
      361 atatttataa gacacaaagt gataaaatct atttagaaaa taatgtaaaa taacaggagg
      421 ataaggcaaa atgaaattta tagtagaaaa agaagttttt gataaattag aaaatgtatg
      481 ttttggtgta gtagttgcaa aaggaataga taatactaaa gaaatagaaa gaataaacaa
      541 tcttttagat ataagtatag atagagtaga agattacttc aaagataaaa aagtaaagga
      601 atcagaagaa attgtaccat atagagaggc atttagaagt ctaggcatga acccaaataa
      661 atttatgagt tcgatagaag caatgacaac aagggttgct aagaataaga aacttcctca
      721 tataaatcct atcgtagatt taggaaactc tatttcttta aaatatttac ttccaatggg
      781 tgctcatgat atggatttta gaaatgatga tgtatatgtt agattttcta aaaaagggga
      841 taaatttgta ccatttggag aaactgatgc tgagttaatg gaagaaggag aattaattta
      901 ttcagttgga gacatggtaa aaactagaag atggatatgg agacaaggag aggaagggaa
      961 aattactaat agctcaaaaa atatattttt tcctatagat ggttttactg atgctaattt
     1021 agataaggta atgagtgcaa gagaagaatt agcaaaattg ttaaaggaaa tatttaattg
     1081 tgaaattaaa gtaggttttg tagataaaga taatcctgaa atggaaatat aataagttgt
     1141 tgaatatttg ttaagatatt atatttattt aatgatgtta aaaaggtatt aaaaaagaaa
     1201 ggtcgtagta gatttgaaaa aaactataat aaagaatgta aatccaatga ttgaaaataa
     1261 gtttatagga ctttttgaaa tagagtataa aaacaaatta gatgaagata aggtttggat
     1321 ggtagcatca agaaaaagta acgagcaatt aaaaaatatt tatttgaaga atgaagaaga
     1381 tagtgtagat gcagttgtaa tagttggact tcataaaagt agtaaaaaat taattttaat
     1441 aagacaattt agagtaccta ttaatggata tatatatgaa ttaccagcag gtcttgttga
     1501 tgaaggagag tctatagaca tttctgttga aagagaactt agagaagaaa caggacttac
     1561 tcttttagaa attaaaaaaa ataaaagtat tgataaagtt tatttatctc ctggaatgag
     1621 tgatgaatct gttgcatttg tttattgtat atgtgatggt gatataacag atgaattttt
     1681 agaaccagat gaagagattg aagcgctttt aatttcgcaa gaagatgcaa aagagatttt
     1741 acaaagtaat cataagatag acacaaaagc atttttgatt ttacaaatgt ttgtgagttt
     1801 aggaactaaa ttatttgaat agatatatag aaaagaggtt atatagaaaa gtaaaataat
     1861 aaagtttatt ttacttgtat aaattataat ctcttttttt atacaataaa gtataatttt
     1921 gtgtattttt aaataagtga cttgactctt aaattttcat aatatgttat aaaccaattt
     1981 cagatactac aataattaaa atcccaaaat tatttaacat atttgcaatc aaaatgagaa
     2041 aaggtaaagg ctgaatgatt gaaaaatttg atgtgaaata gtagattagt ttaatattat
     2101 agtttgtagg gttaaaaatc agaaattgta aaggagataa attctatgaa aaaattagtt
     2161 attattttaa ttagtatcat tagtgtggtt agcttgacag cttgtaaaaa aagcaaggtt
     2221 acttttgaaa gcaatttggt taaggagaaa gaaaaaccct taaaataaat tccagcttgt
     2281 ctataacttg atattcagta cataaaagca gttaatattt atgattgact gctttttctt
     2341 atcaaaaagg ataggttgtg catttttact tgaaatagtt tatacacatc aaaagttggt
     2401 gagcatgttt tttgaaatgg taagtatgaa atcttgataa cacaaggaaa tgaatttaag
     2461 ttttcaatta attatatttc cagaagttag ttaacaataa gctaa
//
LOCUS       DAEPXF010000317         2491 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain HB27
            SAMN13376389-rid12479423.denovo.325, whole genome shotgun sequence.
ACCESSION   DAEPXF010000317 DAEPXF010000000
VERSION     DAEPXF010000317.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13376389
            Sequence Read Archive: SRR10518432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2491)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2491)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001069624.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001069624.1
            Genome Coverage       :: 315x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/15/2021 01:54:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,165
            CDSs (total)                      :: 4,076
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 89
            rRNAs                             :: 10, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 10 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 19 of 69
            Pseudo Genes (incomplete)         :: 44 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 10 of 69
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2491
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13376389-rid12479423.denovo.325"
                     /strain="HB27"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="China: Hebei"
                     /collection_date="2017"
     gene            complement(<1..745)
                     /locus_tag="KRL67_003826"
     CDS             complement(<1..745)
                     /locus_tag="KRL67_003826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease domain-containing protein"
                     /protein_id="HBH1524654.1"
                     /translation="MKYKLFIDFEFNILDDNKNKPKEYNGAELISIGGVLVDNEFNTL
                     DNYYSLVKPKYNKILSNKCKNLTKLNQLDINNAPNLLYVMDDFYKWFCKFDDVTIYNW
                     GDFDITGLLTSFRVYKYTGKCLELFNMMIDIQPFISQHITYNNRILSKQISLLNMKKI
                     FSVEGDIKHNSLSDAVDLMNVCKCFFFNSPKNIDILEELYNKLPALKTPLYYIPYFED
                     ENFELKFDRTPEDIIIYLKQILTILNISKK"
     gene            complement(785..1132)
                     /locus_tag="KRL67_003827"
     CDS             complement(785..1132)
                     /locus_tag="KRL67_003827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH1524655.1"
                     /translation="MLNLKEIRKEIIKKYIGDSLISELTEELKNILITDLQSNPFKSN
                     IELSIIPANIEIFKNIENVLLDSKVNLKMGWERILINYILKDLFELNYEINKSCNEPL
                     MFRVIISIDSYDA"
     gene            complement(1350..2306)
                     /locus_tag="KRL67_003828"
     CDS             complement(1350..2306)
                     /locus_tag="KRL67_003828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HBH1524656.1"
                     /translation="MKKILITGALGQIGSELTIKLRNEYGEQNVIASSRRVKEGNPVC
                     ESGIFEILDVTDKNRFFEIAKKYDVDTIIHLASLLSAVAESKPLEAWNLNMNGLINGL
                     EIAKELDCKFFTPSSIAAFGENSPKNMTPQDTLQRPNTMYGVTKVSGELLCDYYHSKF
                     GVDTRGVRFPGLISYVTPPGGGTTDYAVDIYYEALKNKRYKSYIAEGTKMDMMYMPDA
                     LQSIVDLIEAPADKLIHRNAFNITAMSFSPEEIADSIKKYIPDFVIEYDVDPVRQGIA
                     DSWPNSLDSSSAVKEWNFKFSYDLDKMTEDMLEKLSEKGIGK"
BASE COUNT          843 a          369 c          291 g          988 t
ORIGIN      
        1 cttttttact tatatttagt atagttaata tttgtttaag atatattatt atatcttctg
       61 gagttctatc aaatttgagt tcaaaatttt catcttcaaa gtagggaata taatataaag
      121 gtgttttgag agcaggtaat ttattatata attcttcaag aatatcgata tttttagggg
      181 agttaaagaa aaagcatttg catacgttca ttaaatccac agcatctgaa agtgagttat
      241 gtttaatatc tccttctaca gaaaaaattt ttttcatatt cagtaaagat atttgcttag
      301 ataagattct attattgtat gtaatatgtt gagatataaa aggttgaatg tcaatcatca
      361 tattaaataa ctctaagcat ttacctgtat atttatatac tctaaatgaa gttagaaggc
      421 cagttatatc aaaatcaccc cagttgtata ttgtaacatc atcaaattta caaaaccatt
      481 tataaaaatc atccataaca tataaaagat taggagcatt attgatatcc agttggttca
      541 atttggttaa atttttacat ttatttgaaa gtattttatt gtattttggc ttaactaaag
      601 agtaataatt atctagtgta ttaaattcgt tatcaactaa aactccacca atagaaatta
      661 attccgcacc attatattct tttggtttat ttttattatc gtctagtata ttaaattcaa
      721 agtctataaa taacttgtat ttcataaatc taaagcacat cctcaggtaa attatttatt
      781 gagcctaagc atcataagaa tcaatagata taatgactct aaacatcaaa ggttcattac
      841 aacttttatt tatttcataa tttaattcaa ataaatcttt taagatataa tttataagta
      901 ttctttccca acccattttt aaattaactt tagagtctag taatacattt tctatatttt
      961 taaatatttc tatatttgct ggtattatac tgagttcaat attactttta aacgggttag
     1021 actgtaaatc tgttataagt atatttttta attcctctgt aagttcagaa attaaactat
     1081 cacctatgta tttttttatt atttcctttc taatttcttt taaatttagc aatattagta
     1141 cctgctacct acatagtagc ttttcctttc cttttaatac tattatttat ttttgaatct
     1201 tatttagata tattttaaga ttaaaagtct ttagctatat aattttttac ccatttttaa
     1261 cttatattaa catattacag gcctatatct ccaccctgct tacattgaag atggagtata
     1321 ttgcctattt tgataaaaat gataggttgc tatttaccta ttcctttttc tgataatttt
     1381 tctaacatat cttcagtcat tttatctaaa tcataactaa acttaaagtt ccattctttt
     1441 actgcactac tactgtctag agagttaggc caagaatcag ctataccttg tcttacaggg
     1501 tctacatcat attctattac aaaatcagga atatattttt ttatagaatc agctatttct
     1561 tcaggagaaa agctcatagc agtaatatta aaagcatttc tatggatgag tttatccgct
     1621 ggagcttcga ttaaatctac tattgattga agtgcatcag gcatatacat catatccatt
     1681 tttgtacctt ctgctatgta agatttatat ctcttatttt ttaatgcctc ataatatata
     1741 tcaactgcat aatctgtagt tcctccacca ggaggcgtaa cataagaaat aagacctgga
     1801 aatctaacac ctctagtgtc tactccaaat tttgagtgat aataatcaca tagtaattct
     1861 ccagaaactt tagtcacacc atacatagta ttaggtcttt gtaatgtatc ttgaggagtc
     1921 atattttttg gagaattttc accaaaagca gcaattgaac taggtgtaaa aaatttacaa
     1981 tccaattctt tggctatttc aagaccattt attaatccat tcatatttaa gttccaagct
     2041 tctagaggtt tactttcagc aactgcagaa agtaaagatg ctagatgaat tatagtatct
     2101 acatcatatt tttttgcaat ttcaaaaaaa cgattcttat ctgtaacatc taatatctca
     2161 aatataccag attcacatac tggattacct tcttttactc tccttgaact agcgattaca
     2221 ttttgttctc catactcatt tctaagcttt attgttaatt ctgaacctat ttgtcctaat
     2281 gcacctgtta taagtatttt tttcaatttt acaacctcct aagggtttaa tttatgtagt
     2341 actacttatt tttattttag caaatctttt gtttaaatac aaagaattaa agataataat
     2401 atacgactta attgaatgaa gtgcagtaag tgcctaaaaa tttaaagcta atatataatg
     2461 gtctattgta taacaataaa agagtagatt a
//
LOCUS       DAEPXF010000318         2466 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain HB27
            SAMN13376389-rid12479423.denovo.326, whole genome shotgun sequence.
ACCESSION   DAEPXF010000318 DAEPXF010000000
VERSION     DAEPXF010000318.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13376389
            Sequence Read Archive: SRR10518432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2466)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2466)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001069624.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001069624.1
            Genome Coverage       :: 315x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/15/2021 01:54:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,165
            CDSs (total)                      :: 4,076
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 89
            rRNAs                             :: 10, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 10 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 19 of 69
            Pseudo Genes (incomplete)         :: 44 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 10 of 69
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2466
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13376389-rid12479423.denovo.326"
                     /strain="HB27"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="China: Hebei"
                     /collection_date="2017"
     gene            complement(129..2318)
                     /locus_tag="KRL67_003829"
     CDS             complement(129..2318)
                     /locus_tag="KRL67_003829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid adenylation domain-containing
                     protein"
                     /protein_id="HBH1524657.1"
                     /translation="MYEVNLERAMNYWMDLLDDYTNIAKIPNDFFYKDCSDEVVDEVF
                     KMNIEKHERLIQYSKVNNISIDSIIETIYGLILQKYIYEDDVVFGKTINIIPIRVRVR
                     QSEKFIDVVRKLKRQWSRSLEYGYYLLSDIENKNHLNTKLINTIFIAKSFDLSYDYDI
                     EKKGYDLSVIVYTKRALKIKIKYKPSIYKRETIRRILNQFDYIIEQIIEDNDILIEDL
                     EFVCKEEEKVLLEDFNDNQYEVPYDKSYVDLFREQVKVTPSNIAARDENRKLTYKELD
                     EVTDKLAGYLNHIGVKSEDIVAVMLPRDINIIITAIGIMKSGGAFFPIDTSNPEERLN
                     YLLEDSNAKVVITTDELKSKVVNENTIVLDINNEEMFKLGYELTEKITPSNCAYTIST
                     SGSTGRPKTIAIEHKSLVNMCYYSVKSINATENDICGIYLSFSFDAVMKQLFPYLLVG
                     ASIDIMPEEAKFDEYTVNEYCEENDITILALPTAFARLFIQNCNNNSLRVVQTGGERL
                     KGYRKRNYELYNEYGPTEFTVVSTSFHVDREYGKIPIGKPIFNTYAYVLDKKNKLCPI
                     GAPGELCLSGIQISRGYLNKKGLTEQVFVENPYKTCEHNKLMYRTGDLVRWLDDGNLD
                     YIGRMDNQVKIDEFRIELYEIENIINNITEIKSVVCISRTNDDGDMYICAYYVIDEED
                     SGKINERTIREYLNEHLPPYMIPTIIMRIDKIPVTPIGKVNKKALPE"
BASE COUNT          776 a          446 c          236 g         1008 t
ORIGIN      
        1 aaataaattt tcaccatagt aaatatatac agaaaattct tttttttaca tcaattgatt
       61 ttctcagaaa aataggtgtc ataaagaaat gtatggaatc actttacaac acctgtcata
      121 ataaaaaatc actctggtag tgctttttta tttacttttc ctattggtgt aactggtatt
      181 ttatctatcc tcattattat tgttggaatc atatatggag gtagatgctc atttaaatat
      241 tctctaatag ttctttcatt tatcttacca ctatcttcct catcaattac ataatatgca
      301 catatataca tatctccatc atcatttgtt ctcgatatac aaacaacact tttaatttct
      361 gtaatattat ttattatatt ttcaatctca tatagctcta tcctaaactc atcaattttt
      421 acttgattgt ccatccttcc aatataatct aaatttccat catctaacca tctcactaaa
      481 tcaccagttc tatacattaa cttattatgt tcacaagttt tgtaaggatt ctctacaaat
      541 acttgctctg ttagcccttt tttatttaaa tatcctcttg atatttgaat gcctgataga
      601 cataactctc ctggagctcc aattggacat agtttgttct ttttatccaa tacatatgca
      661 taagtattaa atataggttt tcctattggt atttttccat attctctgtc cacatgaaaa
      721 gaagtcgata caacagtaaa ttcggttggt ccatactcat tatatagttc ataattacgc
      781 tttctatatc cttttaatct ttctcctcca gtctgtacaa ctctaagtga attattattg
      841 caattttgta taaataacct tgcaaacgct gttggcaatg ctagtatagt tatgtcattt
      901 tcctcacaat attcatttac tgtatattca tcgaatttag cttcttcagg cataatatct
      961 attgatgcac ctacaagcaa ataagggaat agttgtttca ttacagcatc aaaactgaaa
     1021 cttaagtata taccacatat atcattttct gtagcgttta tagattttac gctataataa
     1081 cacatattta ctaaactctt atgttctata gctatggtct ttggcctacc agttgaccct
     1141 gaggtagata ttgtatatgc acaatttgat ggtgttattt tttcagttaa ttcatatcca
     1201 agcttaaaca tttcttcatt atttatatct agcactatgg tattctcatt tacaacctta
     1261 cttttgagtt catctgtagt tatcactact tttgcattac tatcttcaag aaggtaattc
     1321 aatctttctt ctggattact tgtgtctatt ggaaaaaatg ctccccctga tttcataatc
     1381 cctattgctg taataattat gttaatatct cttggtaaca ttacagctac tatgtcttca
     1441 cttttcactc ctatatgatt taaataacct gcaagtttat cagttacctc atccaattct
     1501 ttatatgtta actttctatt ttcatcccta gcagctatat tactaggtgt taccttaact
     1561 tgctccctaa ataaatcaac ataactttta tcatagggta cttcatattg attatcatta
     1621 aagtcttcta ataatacttt ttcttcttcc ttacatacaa attctaaatc ttctattaaa
     1681 atatcattat cttctattat ttgctctatt atataatcaa attgatttaa aattcttcta
     1741 atagtctctc ttttgtatat agatggttta tactttattt ttattttaag tgccctctta
     1801 gtatatacta ttacagataa atcatatcct tttttttcta tgtcataatc atatgataaa
     1861 tcaaaacttt tagctataaa tattgtgttt ataagcttag tatttagatg atttttattc
     1921 tctatatctg ataacaaata gtatccatac tctaaagacc tgctccactg cctttttagc
     1981 ttccttacaa catctataaa tttttcactt tgtctaactc ttactcttat tggaattata
     2041 tttattgttt taccaaaaac tacatcatct tcatatatat acttttgaag tataagacca
     2101 tatatagttt ctatgataga gtctatgcta atattgttaa ctttagaata ttgaattaac
     2161 ctttcatgtt tctcaatatt catcttaaaa acctcatcta ccacttcatc agaacaatct
     2221 ttataaaaaa aatcatttgg aatttttgct atattcgtat aatcatctaa caaatccatc
     2281 caataattca tagctcgttc taaattaact tcatacatat caaaattccc ccttactact
     2341 aagtctatag aaaaaattct tttggaattt tattttcaca atatataaat atattaataa
     2401 tacatatgca agctctaaaa acatgagctt gcataaaatt acacttatta tggctactaa
     2461 attcgt
//
LOCUS       DAEPXF010000319         2431 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain HB27
            SAMN13376389-rid12479423.denovo.327, whole genome shotgun sequence.
ACCESSION   DAEPXF010000319 DAEPXF010000000
VERSION     DAEPXF010000319.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13376389
            Sequence Read Archive: SRR10518432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2431)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2431)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001069624.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001069624.1
            Genome Coverage       :: 315x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/15/2021 01:54:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,165
            CDSs (total)                      :: 4,076
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 89
            rRNAs                             :: 10, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 10 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 19 of 69
            Pseudo Genes (incomplete)         :: 44 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 10 of 69
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2431
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13376389-rid12479423.denovo.327"
                     /strain="HB27"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="China: Hebei"
                     /collection_date="2017"
     gene            complement(<1..1577)
                     /locus_tag="KRL67_003830"
     CDS             complement(<1..1577)
                     /locus_tag="KRL67_003830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HBH1524658.1"
                     /translation="MMFKIFNKSLLKLVVVLSIVLTISNFSLSSYASEITSSTDKVLI
                     IYDSKKETAYNRDILNIMRTLLGRFSSDIELLKLSNYDGEINKNYYSHIFILGINENS
                     YDNDKNTKNLISSLNSYKGTICWLGYGIENLLEHKKYNLDYVGKTNNIVSVNYRGKSY
                     NLDEHYVFNIVESKDTNNKVIGSINDTLNKYPYIINDKNLFYVSKLDLDGVLFYIFCD
                     SLNDIFNIKTFDKGRIFVRIEDVHAFREPKNLVEIADYLSSKNIPFTIALIPAYVNPK
                     NHKVITLSESPEIVKAIKYMQDKGGTVILHGYTHQYKKEEVSGEGYEFWDGKKDEPLK
                     ENMKIFVKDRVLNGLRVCIENGIYPLAFEAPHYAMESEGYKELKKYFSTYMGQHQNND
                     KKFSTNTYPYIIRDTEEFNIFIPENLGYIDPEDKFTFQNIKENLDKLSIVRGFSGGFF
                     FHSYLNIEYLKNTIEYLEKQNIEFMNLRDFNNWVKVDEIQIKNNGDEIIVNYDKDLDE
                     ITKSDTRFKSISNISKI"
     gene            complement(1567..2415)
                     /locus_tag="KRL67_003831"
     CDS             complement(1567..2415)
                     /locus_tag="KRL67_003831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HBH1524659.1"
                     /translation="MNRINKKIDLLILALLVFIFLIVGIVILSIRTENKLNVFIMLAI
                     IFFIIMLTYLSNSVVGLITSSIIIFMYTSYILYNNITHNMDVEFISYMWIIAIPISSI
                     IMGNLNKSINELQNTNKKLSEQYKELVTIDSETGLRNLKIFYNDVNMEISKSIRHNTD
                     FSLMIVKLPYYGNLQTIFGENKTNKIVKYIGSNIIECTRNEDIIYSLQKDMIGILMPN
                     TSLEGSKVVKDRIKKRIKELNLDLNNRGKYVNIDVKIAFLQYKSSFGDSINFKNTVEE
                     ELQYDV"
BASE COUNT          805 a          367 c          218 g         1041 t
ORIGIN      
        1 aaaattttac taatattgga tatggattta aatctagtat cacttttagt tatttcatct
       61 aagtccttat catagttgac tataatttca tctccattat tttttatttg tatttcatca
      121 acttttaccc aattattaaa atctcttaaa ttcataaatt ctatattctg tttttctaaa
      181 tattctattg tgttttttag atactctata tttaaatatg aatgaaagaa gaaccctcca
      241 ctaaaacctc ttacaatgga taatttatct aaattttctt ttatattttg aaaagtaaac
      301 ttatcttctg ggtctatata tcctaaattt tcgggtataa atatattaaa ctcctctgta
      361 tccctaataa tataaggata tgtatttgtg ctaaattttt tatcattatt ttgatgttgc
      421 cccatataag tagaaaaata tttttttaat tctttatatc cctctgattc cattgcataa
      481 tgtggcgctt caaatgcaag aggataaatt ccattctcta tacatactct aagaccattc
      541 aaaactctat ctttgacaaa tatcttcata ttttctttta agggttcatc ttttttacca
      601 tcccaaaatt catacccttc tccagaaact tcttctttct tgtattgatg agtatatcca
      661 tgcaatatca cagttccacc tttatcttgc atatatttta ttgctttgac aatttcaggg
      721 ctttctgata aagttattac tttatgattt ttaggattta catatgctgg tattaatgca
      781 atcgtaaatg gtatattctt actacttaaa taatccgcta tctctacaag attttttggt
      841 tctctaaaag catgtacatc ttctattctt acaaatatac gtcctttatc aaacgtctta
      901 atattaaaaa tatcgttaag tgaatcgcaa aatatataaa ataaaactcc atctaaatct
      961 aattttgata cataaaaaag atttttatca tttattatat atggatactt atttaaggta
     1021 tcattaatac ttcctattac tttattattt gtatcttttg attctacaat gttaaaaaca
     1081 taatgttcat ccaagttata tgatttacct ctataattta cactaactat attgtttgtt
     1141 tttcctacat agtctaaatt gtatttttta tgttccaaaa gattttctat accatatcct
     1201 aaccaacata tagttccttt atatgaattt aaggaactaa tcaagttttt agtgttttta
     1261 tcattatcat atgagttttc attgattcct aaaataaata tatgactgta ataattttta
     1321 tttatctcac catcatagtt actcaatttt aatagctcta tatctgaact aaatcttccc
     1381 aatagagtcc tcataatatt taaaatatct ctattgtaag ctgtttcttt cttagaatca
     1441 tatatgatta ataccttatc tgtgctggaa gtaatttctg atgcatatga agataagcta
     1501 aaatttgata ttgtcagtac tattgataat acgactacta attttaataa acttttattg
     1561 aatattttaa acatcatatt gaagttcctc ttcaactgta ttcttaaaat ttatactatc
     1621 tccaaaacta ctcttatatt gtaaaaatgc aattttgaca tctatattta catattttcc
     1681 cctattgttt aaatctaaat ttaattcttt tatccttttc tttattctgt ctttaacaac
     1741 ttttgaacct tccaaagatg tatttggcat tagtatccct atcatatctt tttgtaaact
     1801 atagattata tcttcatttc ttgtgcattc tataatattt gaaccaatat attttactat
     1861 tttattagtt ttgttttcac caaatatagt ctgtagattt ccgtagtacg gtaattttac
     1921 tatcataaga gaaaaatcag tattatgtct aatagattta cttatctcca tatttacatc
     1981 attataaaaa atctttaaat ttctaagtcc agtttcacta tcaatagtca ctaattcctt
     2041 atattgttca gatagttttt tatttgtatt ttgcaattca tttatacttt tgttcagatt
     2101 acccattata attgaagata ttggtattgc tattatccac atatatgata taaattcaac
     2161 atccatattg tgagtaatat tgttatataa aatataggat gtatacataa atataattat
     2221 tgaacttgtt atcaatccca ctactgaatt tgataaataa gttaacatta ttataaaaaa
     2281 aattattgct agcattatga acacattcag tttattttct gtcctaatcg atagtattac
     2341 tattccaaca attaaaaata taaaaacgag caaagctaat attaataaat ctattttttt
     2401 atttatccta ttcatttttt ataaatttac a
//
LOCUS       DAEPXF010000320         2418 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain HB27
            SAMN13376389-rid12479423.denovo.328, whole genome shotgun sequence.
ACCESSION   DAEPXF010000320 DAEPXF010000000
VERSION     DAEPXF010000320.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13376389
            Sequence Read Archive: SRR10518432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2418)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2418)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001069624.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001069624.1
            Genome Coverage       :: 315x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/15/2021 01:54:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,165
            CDSs (total)                      :: 4,076
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 89
            rRNAs                             :: 10, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 10 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 19 of 69
            Pseudo Genes (incomplete)         :: 44 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 10 of 69
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2418
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13376389-rid12479423.denovo.328"
                     /strain="HB27"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="China: Hebei"
                     /collection_date="2017"
     gene            <1..1259
                     /locus_tag="KRL67_003832"
     CDS             <1..1259
                     /locus_tag="KRL67_003832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HBH1524660.1"
                     /translation="IFSIHEITLNTENFKRARKESVNLIKGKIIRTIIYSLILSLVVA
                     IIGYVIYHSGVIFIGIWTKYYTDMEFLKNIFINRCIAFEEYFRLILSIFIFILDIGFI
                     SATYYQYKGVSISKNNIKKEKKSISKIVFNLVLILLISYIESVAFSSHVNGMFNTSFS
                     FYTTAIAHRAGAEMAPENTLAAIEEASHAKADYAEIDVQETKDGELIVLHDSNFKRTT
                     GVDKNVWDVNYNEVKTYDAGSFYYYGKGYYDEKFVGEKIATLKEMIKYSKGKVKLLIE
                     IKLNGHEKGDVVKKVVQLIKENNFENQCIVASMDKTILQKVKKLDENLLTCYFMAIAY
                     GDFSKLNYVDIYGIESTFVNKTVVEKIHKMNKKIFVWTVNSDSLIEKMLDLNVDSIIT
                     DNPYLIESAIYWKKNGFISQVSDYLF"
     gene            1580..2164
                     /locus_tag="KRL67_003833"
     CDS             1580..2164
                     /locus_tag="KRL67_003833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HBH1524661.1"
                     /translation="MTEREKMLAGELYDCGDIELLTQWHRAKDLVRDYNQTDSSDLEK
                     KEKILNELLGGKGKNLWITAPYYVDYGNNIYFGNNCEVNMNCTFLDDNKIIIGDNVLI
                     APNVQIYTAFHPTNAGDRFGNAKEDGSFEFCKTQTAPVTIGDNVWIGGGAIIMPGVTI
                     GDNVVIGAGSIVTKDIPSNMIAYGNPCRIIRENK"
BASE COUNT          943 a          236 c          392 g          847 t
ORIGIN      
        1 ggatttttag cattcatgag ataactttaa atacagaaaa ttttaaaaga gcaagaaaag
       61 aaagtgttaa tcttataaag ggaaagatta taagaacaat tatttattca ttaatactat
      121 ctttagtagt agctattatt ggatatgtga tttaccatag tggagttata tttataggca
      181 tatggactaa atattatact gatatggaat ttttgaagaa tatatttata aatagatgta
      241 ttgcatttga agaatatttt agattgattt tatcaatatt catttttata ttggacattg
      301 gatttatctc agcaacttat tatcaatata aaggagtaag tatttctaaa aataatatta
      361 aaaaagagaa aaaatctata tcaaaaattg tttttaattt agtattaata ttattaattt
      421 cttatataga gtcagttgca ttttcttcac atgtaaatgg tatgtttaat acgtcatttt
      481 cgttttatac aacagcaatt gctcatagag caggagctga aatggctcca gagaatactt
      541 tggcagcaat agaagaagct tcacatgcta aagctgatta tgcagaaata gatgttcaag
      601 aaactaaaga tggagaactt attgtacttc atgattcaaa ctttaagaga actacaggag
      661 tagataaaaa tgtttgggat gtaaattata atgaagtaaa gacatatgat gcgggaagct
      721 tttattatta tggaaaaggt tattatgatg aaaagtttgt tggggaaaag atagcaacac
      781 tcaaagaaat gataaaatat tctaaaggta aggtgaaatt attaatagaa ataaaactta
      841 atggacatga aaagggagac gtagtaaaaa aagttgttca gttaattaaa gaaaataatt
      901 ttgaaaatca atgtatagtt gcatctatgg ataaaactat attacaaaaa gtaaaaaaac
      961 tggatgagaa tttactgaca tgctatttta tggcaattgc atatggtgat ttttcaaaat
     1021 taaattatgt tgatatatat ggaattgaat caacttttgt taataaaaca gtagtagaaa
     1081 aaattcataa aatgaacaaa aaaatcttcg tttggaccgt taatagtgat agtttaattg
     1141 agaaaatgct tgacctaaat gtagatagta ttattacaga taatccatac cttatagaga
     1201 gtgcaattta ttggaagaaa aatggattta ttagtcaagt gtcagattat ttattttaaa
     1261 tattctataa ttgtattatc cagtaaaaga gatattatta aaatcaataa tatctctttt
     1321 taacataaac aagtttgtta aattaaggct attcttataa aaattgtgct tatcagctat
     1381 aaaaaggaat aaaagtgaaa aaattgccat gacattatta caattaaaat agttttaata
     1441 tgatagagag gtagattgtc aactgtatat ttaataaatc ttgttattta ttaaatttta
     1501 tataagtaaa atatatagat atataaatct tatgctagaa tatatatgaa aaacaatata
     1561 aatttggagg attaaaaaca tgacagagcg agaaaaaatg ttggcaggag aactttacga
     1621 ttgtggtgat attgagctgt taactcaatg gcatagagca aaagacctag taagagatta
     1681 taaccaaaca gattcctcgg atttggaaaa aaaagaaaaa atattaaatg aattacttgg
     1741 tggtaagggt aaaaacttat ggattactgc accgtattat gtagattatg gaaataatat
     1801 ttattttgga aataattgtg aagtcaatat gaattgtacg tttttagatg ataataaaat
     1861 tatcattggg gataatgtac taattgcacc aaacgtacaa atatatactg catttcatcc
     1921 aactaatgct ggtgatagat ttggtaatgc caaagaagat ggttcatttg aattttgtaa
     1981 aactcaaaca gctccagtta ctattggaga taatgtttgg attggcgggg gtgctattat
     2041 catgccaggt gttactattg gagataatgt agttataggt gcaggaagta ttgttacaaa
     2101 ggatattcca agtaatatga ttgcatacgg aaatccatgt cgtattattc gagaaaacaa
     2161 ataaagttaa attttcttgc aaaatctcaa aaaacacctt tcttacttta tacatagggt
     2221 attttccaat actttcacat ccatattcat ttgttaaatt tttgtggaat ttagttttta
     2281 tcacttctat cttacatttc caccattttt tataaaagtc atccctttat agatttattt
     2341 ataatagtat atattctatt tgatgtttga ataagcactt atttttaagt aaaatactaa
     2401 cttttaggtt agtatttt
//
LOCUS       DAEPXF010000321         2413 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain HB27
            SAMN13376389-rid12479423.denovo.329, whole genome shotgun sequence.
ACCESSION   DAEPXF010000321 DAEPXF010000000
VERSION     DAEPXF010000321.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13376389
            Sequence Read Archive: SRR10518432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2413)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2413)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001069624.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001069624.1
            Genome Coverage       :: 315x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/15/2021 01:54:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,165
            CDSs (total)                      :: 4,076
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 89
            rRNAs                             :: 10, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 10 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 19 of 69
            Pseudo Genes (incomplete)         :: 44 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 10 of 69
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2413
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13376389-rid12479423.denovo.329"
                     /strain="HB27"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="China: Hebei"
                     /collection_date="2017"
     gene            283..2178
                     /locus_tag="KRL67_003834"
     CDS             283..2178
                     /locus_tag="KRL67_003834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp8"
                     /protein_id="HBH1524662.1"
                     /translation="MLSNKKRSMAIVIAGATVMSAAAPIFADNTVTENVDKNYTVSAK
                     DSAKLIEEVRKALEVKFEDTKAGANVNDRVYDIKVDNVNLTNATQLQNKINSLTEGQS
                     LKVTIQDKGHQVLGGKVVDYKIENYKTAQEIVDAVNAYNATLAEDSDNKLTATIKSTN
                     TVEVKRAKDSTNVITLNVGDQHLDFSKVITSEEGTFEGYEKSYSDIDSKELHTITVKN
                     ADLQDISAEELFDGIRLTTLGREIVNKVKNGYALTFENEAILTQEQEDSDDKDKPEKS
                     SFDIVLSKANEKPETISVSSKNHKLVRDLHKVLTDVKDGKELKVEVLSGDSRFTTAVE
                     VSKERFKDGEAEAIILVGEDAIVDGLASAPLASQKNAPILLSKKDSLPSEIEAEILRV
                     LGSNLSSKKIYIVGGESKVSKETEEKLSKLGVSKVERVSGEDRFETSLEIAKQLKDTF
                     KTAFVVGGNGEADAMSISARAAQFGAPIIVTGNELDANAEKLLKGKELEIVGGENSVS
                     KEVEDKLVDIDLNNKVERLAGENRKDTNAKVINKYYAGATKAYVAKDGYVGGNGQLVD
                     ALTAAPLAASSKAPIVLTTEELSKSQEEVVELRLKNATKLVQIGEGIAKNAIEKIAEK
                     INLFTKN"
BASE COUNT         1055 a          245 c          435 g          678 t
ORIGIN      
        1 agaaaaaatt agtgtatagt agtatataaa aatggagttt aaatactttt gttagtatat
       61 gattgtttat tagaaaatca ctataaaata gggatttcta tctttgagca tgattataga
      121 caactaagtg gggagggggt tcaagaagat attattagtg tttgtgttag taaattatgt
      181 aaacacataa aatatattga aaaataatat tatatataat attatttatg tgtgggaaat
      241 acgaatataa aaaataaaaa ataatattcg gaggacataa atatgttaag caacaaaaag
      301 agaagtatgg caatagtaat agcaggtgct acagttatga gtgcagcagc accaatattc
      361 gcagacaata ctgtgacaga aaatgttgat aaaaattaca cagtaagtgc taaagattct
      421 gctaaactaa tcgaagaggt tagaaaagca ttagaagtta agtttgaaga tacaaaagca
      481 ggagcaaatg taaatgatag agtttacgat ataaaagtag acaatgtaaa cttaactaat
      541 gcaactcaat tacaaaataa aataaactct ttaacagaag ggcaaagttt aaaagttact
      601 attcaagata aaggtcatca agtgttaggt ggaaaagtag ttgactataa aattgaaaac
      661 tacaaaactg ctcaagaaat agtagatgca gttaatgctt acaatgcaac tttagcagaa
      721 gattctgata acaagttaac agcaactata aaatctacaa atacagttga agtaaaaaga
      781 gcaaaagatt caacaaatgt tattacatta aatgtaggag accaacattt agatttctca
      841 aaagtgataa catcagaaga aggtactttt gaaggttatg aaaagagtta tagtgacata
      901 gattcaaaag aattacatac gataacagtt aaaaacgcag atttacaaga catatcagca
      961 gaagaattat ttgatggaat tagattaact acattaggaa gagaaatagt taataaagtt
     1021 aaaaatggat atgctttaac atttgaaaat gaagcaatcc ttactcaaga acaagaagat
     1081 agtgatgata aagataagcc agaaaaatca agttttgata tagttttaag caaggcaaat
     1141 gaaaagccag aaacaataag tgtttcaagt aaaaaccata agttagttag agacttacac
     1201 aaagtattaa ctgatgtaaa agatggaaaa gaattaaaag tagaagtttt atctggtgat
     1261 tcaagattta caacagcagt agaagttagt aaagagagat tcaaagatgg agaagcagaa
     1321 gctataattt tagttggaga agatgctata gttgatggat tagcatcagc accacttgcc
     1381 tctcaaaaaa atgcaccaat attattatct aaaaaagatt cactaccatc agaaatagaa
     1441 gctgaaatat taagagtact tggaagtaat ctatcttcta agaaaatata tatagtaggt
     1501 ggagaatcta aagtatcaaa agaaactgaa gaaaaacttt ctaaattagg tgtaagtaaa
     1561 gttgagagag tttctggaga agatagattt gaaacttctt tagaaatagc aaaacaatta
     1621 aaagatacat ttaagactgc ttttgtagta ggtggaaacg gagaagctga tgctatgagt
     1681 atatcagcta gagctgctca atttggtgct ccaataatag ttacaggtaa cgaattagat
     1741 gcaaatgctg aaaagttatt aaaaggaaaa gaattagaaa tagtaggtgg agaaaattct
     1801 gtatcaaaag aagttgaaga caaattagtt gatatagatt taaataataa agttgaaaga
     1861 ttagctggag aaaatagaaa agatactaat gctaaagtaa tcaataagta ctatgcaggt
     1921 gcaactaaag catatgtagc aaaagatggt tatgtaggtg gaaatggaca attagttgat
     1981 gcacttacag cagcaccact tgcagctagt tcaaaagctc caatagtatt aactacagaa
     2041 gaactttcta aatcacaaga agaagtagtt gagttaagac ttaagaatgc tactaagtta
     2101 gtacaaatag gtgaaggaat agctaaaaac gctattgaaa aaatagcaga aaaaataaat
     2161 ttatttacta agaactaata agatatagga attaatatta aaagtttatt tagataatat
     2221 agattataca atgtgttaat atgtaagaca tagtgtagat attataaaat atacaaataa
     2281 caaaataaaa ataaggtgtc tcatttagaa ttagattgag ataccttatt ttattttttg
     2341 attataatgt tgagaaactc atattgcaaa aattattata aaaagatact tcttataatg
     2401 attaaacatc tta
//
LOCUS       DAEPXF010000322         2367 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain HB27
            SAMN13376389-rid12479423.denovo.330, whole genome shotgun sequence.
ACCESSION   DAEPXF010000322 DAEPXF010000000
VERSION     DAEPXF010000322.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13376389
            Sequence Read Archive: SRR10518432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2367)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2367)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001069624.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001069624.1
            Genome Coverage       :: 315x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/15/2021 01:54:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,165
            CDSs (total)                      :: 4,076
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 89
            rRNAs                             :: 10, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 10 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 19 of 69
            Pseudo Genes (incomplete)         :: 44 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 10 of 69
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2367
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13376389-rid12479423.denovo.330"
                     /strain="HB27"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="China: Hebei"
                     /collection_date="2017"
     gene            56..973
                     /locus_tag="KRL67_003835"
     CDS             56..973
                     /locus_tag="KRL67_003835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH1524663.1"
                     /translation="MENNKCREDFRFTQEYEEDYPNTNERYYENYQVADRYYNYPNKY
                     KEPKMKQCCCKKSMREALELLRYDALRPFVNFNQFAFISDFFIVGAYLVGIDYSIPPK
                     DNLSGLNGTFERFSACNCDLIDISGQLYYPKPVPLTLGGLVTSIGTIPGVSELVALIE
                     AAVPATIDLGAILNAILDDIIDYIISTVNPLTNVDLASLCSLKAVAFNVTPADYEDFV
                     ASLGYYLDKKHYKECNCNCDCDDCCCNKGILDNLYMSNINNQVTVVAGSLVLTGVEVL
                     GKKNDVLVLGNSNDSRIYFVCVDSIDYIA"
     gene            complement(1114..2295)
                     /locus_tag="KRL67_003836"
     CDS             complement(1114..2295)
                     /locus_tag="KRL67_003836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HBH1524664.1"
                     /translation="MNKKYIKAGNLIDGTGNSIMQNKGIIIDGDTIVEIEDIRADLNE
                     YDVYDYLDKTVMPGIMNCHVHLTMEPVGNPKVYYDNVSDVELVVNTIKQLDAYLDSGV
                     TYIRSLGCPKYVDVQLKNLIDKGSIDGPGIVTSGPVICMTGGHGYYFGIESDGVDECR
                     KSARTVLKNGVDCVKIMATGGVTTDGVEPGSPQLTLEEMKAAVDEAIKAGKITATHAQ
                     GRTGIKNAVLAGITSIEHGVYLDDEIIDLMLERGTYLVPTVAAPYFILNAGKESGITE
                     ATLRKCEVVAIPHKESFLKAYKKGIKIAVGSDAGTSYNEHGKTYYEMKLMSDYGMDNM
                     DVIVAATKTASELLKIDKNYGTLEKGKKADVIVVNGNPIEDIDVLADVLAVFKLGKKV
                     R"
BASE COUNT          760 a          403 c          307 g          897 t
ORIGIN      
        1 tagataaact taattatcta tacatttaaa tcaaaatata aaaggagtgt atttaatgga
       61 aaataataaa tgtagagagg actttagatt tactcaagaa tacgaggaag attatccaaa
      121 tacaaatgaa agatactatg aaaattatca agtagctgat agatactata attatccaaa
      181 taaatataaa gaacctaaaa tgaaacaatg ttgttgtaaa aaaagtatga gagaggcatt
      241 agaacttcta agatatgatg ctctaagacc ttttgtaaat tttaatcaat ttgcctttat
      301 ttcagatttc tttatcgttg gtgcttattt agtaggtata gattattcaa tccctccaaa
      361 agataattta tctggactta atggtacttt tgaaagattt tcagcttgta actgtgattt
      421 aatagatata tctggtcaat tgtattatcc taagcctgtt cctttgacac ttggaggatt
      481 agttacttca attggtacta ttccaggagt atctgaatta gttgctctta tagaagcagc
      541 agttcctgct actatagacc ttggagctat attaaatgca atacttgatg atataattga
      601 ttatataatt tctacggtta acccactaac aaatgtagat ttagcatcat tatgtagtct
      661 taaagctgtt gcctttaatg ttacacctgc agattatgaa gattttgtag catctttagg
      721 ttactatctt gacaaaaaac attacaaaga atgtaattgc aactgcgatt gtgatgattg
      781 ctgttgtaat aaaggtatcc tagataatct ttatatgtca aatataaata atcaagttac
      841 tgtagtagct ggtagtttgg ttctaactgg tgttgaagtt ctaggtaaga aaaatgatgt
      901 tttagtactt ggaaattcta atgattcaag aatatacttt gtatgtgtag attctataga
      961 ttatattgca tagaatgatt ttaaaaagct atatctgaat atagaatagc attcattttc
     1021 taaagtataa aaaatttaat tattattaat aaaaaagagt gcctataatt attaaaaatc
     1081 cctttagaca ctctttttta ttttttatgc tacttatcta acttttttac caagtttaaa
     1141 tactgctaat acatctgcaa gtacatctat atcctctatt ggatttccat ttacaactat
     1201 aacatctgct ttctttcctt tttctaaagt tccataattt ttatcaattt ttaaaagctc
     1261 agatgctgtt tttgtagcag caactattac atccatatta tccatcccat agtctgacat
     1321 tagtttcatt tcataatatg tttttccatg ttcattataa gaagttcctg catcagaacc
     1381 aactgcaatt ttaattcctt ttttgtatgc ttttaggaat gactccttgt gtggaatagc
     1441 aacaacttca cactttctaa gagtagcttc agttattcca ctttcttttc ctgcatttag
     1501 aataaaatat ggtgcagcca cagttggaac taagtaagtt cctctttcaa gcatcaaatc
     1561 aattatttca tcatctagat aaacaccatg ttctatagag gtaattcctg caagcactgc
     1621 attttttata ccagttcttc cttgtgcatg tgtggcagtt attttacctg cttttatagc
     1681 ttcatctaca gcagccttca tttcctccaa agttaattgt ggtgaccctg gctcaactcc
     1741 atcagttgtt acacctccag ttgccattat ctttacacaa tctactccat tttttaaaac
     1801 tgttctggct gattttctac attcatccac tccatcactt tcaataccaa aatagtatcc
     1861 atgtcctcct gtcatacata taactggacc tgaagtaact ataccaggac catctataga
     1921 acctttgtct ataagatttt taagctgtac atccacatat ttgggacaac ctaagcttct
     1981 aatatatgtt actcctgaat ccaaataagc atcaagttgt tttatcgtat ttactacaag
     2041 ctctacatct gatacattat cataatatac ctttggattt ccaactggct ccattgtcaa
     2101 gtgaacatga caattcataa tgcctggcat aacagtttta tctaagtaat cgtacacatc
     2161 atattcattt aaatctgctc ttatatcttc aatttcaact atagtatctc catcaataat
     2221 tataccctta ttttgcataa tactatttcc tgttccatca attaaattcc ctgctttaat
     2281 atattttttg ttcatcttaa atttctcccc cttttaagta caatgtatta tttatttatt
     2341 atttctaata ttctaataat taatttt
//
LOCUS       DAEPXF010000323         2296 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain HB27
            SAMN13376389-rid12479423.denovo.331, whole genome shotgun sequence.
ACCESSION   DAEPXF010000323 DAEPXF010000000
VERSION     DAEPXF010000323.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13376389
            Sequence Read Archive: SRR10518432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2296)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2296)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001069624.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001069624.1
            Genome Coverage       :: 315x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/15/2021 01:54:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,165
            CDSs (total)                      :: 4,076
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 89
            rRNAs                             :: 10, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 10 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 19 of 69
            Pseudo Genes (incomplete)         :: 44 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 10 of 69
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2296
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13376389-rid12479423.denovo.331"
                     /strain="HB27"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="China: Hebei"
                     /collection_date="2017"
     gene            complement(155..1423)
                     /locus_tag="KRL67_003837"
     CDS             complement(155..1423)
                     /locus_tag="KRL67_003837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH1524665.1"
                     /translation="MKAKNIGKYTICFIIVCFLMITMTLILSYFNKDKLSVKTLKGNV
                     KDIGRVSVVYTPNISKFSYEEVIINKNGVRNDTNPKSRIRNDYLNIDKKYSDFVNDKG
                     KELIYKDDLNIGEAYTSVKSDLVQGMNALNDCIKVSNKNLKSGVEENFDINLNKSDFK
                     ENSWKTLFNKIYKGNLYMVISETNISGSKVNNICILKIDIKNKSYKLLNRFNLGLQNG
                     YQQNINEIKFSIGSKLYLEIVNNKSKNGDIEGHDSYSNLIIYDIEENTFNISNKFMDN
                     LNKEKNANPKEIGLLSKTKLDYMVKNNKLNLIVTDMSNSITNLVYDINSNEIKLEDYD
                     ILDLKVDYNEASSGVRKVKLLDDKIYSISGIKNSISGELKGFKGVVDIVGTKPVKFQV
                     FDMKLKRNVYEAELINGDISLQEKLFFVAN"
     gene            complement(1434..2246)
                     /locus_tag="KRL67_003838"
     CDS             complement(1434..2246)
                     /locus_tag="KRL67_003838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH1524666.1"
                     /translation="MKDLIRLFEFELKRNIKNYIFIIICCCSFVILNIVKNLNDYNYI
                     IENAVKSEQLTKIGNTIETVNVIGFSSFRNIMGSTESWFMFGIIACICYAFFIWYRDF
                     NGRSKSIYTLIMLPKNRINIYISKLLNILFLVYSYTVVLTISLFITSKLLPRHMLGNV
                     TSYGFVQETIYELKMLLPYNFEILFVEYIFLLIGFVSVVFTAILINKSIYKVSTLISF
                     LFLILEILVFIISIEFIIPYEYSDVFTVIYSSINIFVCSLISNKLLNQKIDF"
BASE COUNT          832 a          344 c          176 g          944 t
ORIGIN      
        1 tcactatcaa aataaatcta aacaatgaaa atatatttga tttcatataa aaaaagtgtc
       61 tcatgagcta aaaagtttat tttgagacac tcccttactt atatttatct gtaatgcata
      121 taaaagttta aaattctaaa attaattcta tcatttaatt tgcaacaaaa aataactttt
      181 cttgtaaact tatatctcca tttattagct cagcttcata aacattcctt ttaagtttca
      241 tatcaaatac ttgaaatttt actggcttag ttccgactat atcaacaacc cctttaaatc
      301 cttttaattc tcctgatata ctgttcttaa tacctgaaat agaatatatc ttatcatcaa
      361 gtaacttaac tttcctaact ccagatgatg cttcattata atctactttt aaatcaagta
      421 tatcataatc ttctaattta atctcattag aattaatatc ataaacaaga ttggttatgg
      481 aattgctcat atctgtaact ataagattca atttattatt ttttaccata taatctagct
      541 tagttttact taataagcca atctcctttg gatttgcatt cttttctttg tttagattat
      601 ccataaattt attacttata ttaaaagtgt tctcttctat atcatatata ataaggttag
      661 agtaactatc atgtccttca atatctccat ttttactttt attatttact atttctaagt
      721 ataatttact tccaatagaa aatttaattt cattgatatt ttgttgatat ccattctgta
      781 atcctaaatt aaatctattt aataatttat aagacttatt ttttatatct atctttaata
      841 tacaaatatt attgacctta gaacctgaaa tattagtttc actaattacc atatataaat
      901 tgcctttata tattttatta aacaaggttt tccaactatt ttctttaaaa tcagatttat
      961 ttaaattaat atcaaaattt tcttctaccc cactttttaa atttttattt gaaaccttta
     1021 tgcaatcatt taaagcattc attccttgta ctaaatcaga cttaacagaa gtatacgctt
     1081 ctcctatatt caaatcatct ttatatataa gttcttttcc tttatcatta acaaaatcac
     1141 tgtatttttt gtcaatattt aaataatcat ttctaattct tgattttggg tttgtatcat
     1201 ttcttactcc atttttattt ataattacct cctcataaga aaatttgctt atattaggtg
     1261 tatatactac acttactctc cctatatctt ttacattgcc ttttaaagtt tttacagata
     1321 gtttatcctt attaaaataa ctcaatatta aggtcatagt aatcattaaa aaacatacta
     1381 ttataaaaca tattgtatat tttcctatat tttttgcttt cataatatcc ctcctaaaag
     1441 tcaatctttt gattcaaaag tttattagat ataagactac atacaaatat gtttatactt
     1501 gaatatataa cagtaaatac atcactgtat tcgtaaggta taataaattc tatagaaatt
     1561 ataaacacta aaatttccaa aattaaaaat aagaaactta ttaaagtgct tactttataa
     1621 atagatttgt ttataagaat tgctgtaaat actacagata caaatcctat caataaaaat
     1681 atatactcta caaatagtat ttcaaagtta tatggaagta acatttttaa ctcatatatt
     1741 gtttcttgta caaaaccata gcttgtgaca ttaccaagca tatgtctagg taacaactta
     1801 cttgtaataa ataaagatat tgtaagtact actgtatagc tatacactag aaataatata
     1861 tttaataatt tagatatata aatattgatt ctatttttag gtagcattat aagtgtatat
     1921 atagacttac tcctaccatt aaagtctcta taccaaataa aaaaagcata gcaaatacat
     1981 gcaattatac caaacataaa ccacgattct gtagaaccca ttatatttct aaagcttgag
     2041 aaacctataa catttactgt ttctatagta tttccgattt ttgttaactg ttcactttta
     2101 actgcatttt ctattatgta attatagtca tttaaatttt ttactatgtt taaaattaca
     2161 aaagagcaac aacaaattat tataaatata taattcttta tatttctttt taattcaaat
     2221 tcgaataatc ttattaaatc tttcatcaac tcacctactt tatttcaaat tctaacgtac
     2281 tatttgaact gttaaa
//
LOCUS       DAEPXF010000324         2241 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain HB27
            SAMN13376389-rid12479423.denovo.332, whole genome shotgun sequence.
ACCESSION   DAEPXF010000324 DAEPXF010000000
VERSION     DAEPXF010000324.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13376389
            Sequence Read Archive: SRR10518432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2241)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2241)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001069624.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001069624.1
            Genome Coverage       :: 315x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/15/2021 01:54:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,165
            CDSs (total)                      :: 4,076
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 89
            rRNAs                             :: 10, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 10 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 19 of 69
            Pseudo Genes (incomplete)         :: 44 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 10 of 69
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2241
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13376389-rid12479423.denovo.332"
                     /strain="HB27"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="China: Hebei"
                     /collection_date="2017"
     gene            7..1458
                     /locus_tag="KRL67_003839"
     CDS             7..1458
                     /locus_tag="KRL67_003839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine:cation symporter family protein"
                     /protein_id="HBH1524667.1"
                     /translation="MDFFNGILTSINNAVVFVNNYLWSYILIIMLVVIGIYFTIKTNF
                     VQFRYFIEMFRLLGDGTANKNAKKEGKISSFQAFCISTSSRVGTGNIAGIAIAVVAGG
                     PGAVFWMWLIALIGSASSFVESTLAQIYKVKNGQAFRGGPAYYMEQGLNKKWMGVLFS
                     ILITICYGFVFNAVQANTVSLAFNNAFGISKMTMGIGLGILTAIVIFGGVHRVAKVSE
                     IIVPVFAGLYILVALAIVVMNITEIPSVIALIVESAFDFKGMAIGTFMGVVMTGVKRG
                     LFSNEAGMGSAPNAAATAHVTHPVKQGLIQSLGVFTDTIIICSCTAFMVLLYSGYSSS
                     GATGIELAQEALTHHIGPVGNIFIAVCIFLFAFSSIVGNYYYGESNMEFMSGSKTKLN
                     VFRVFVVGMVLFGSLTQVDVVWNLADLFMALMAIINLIAIALLGKYAFIALKDYTSQK
                     KSGIKDPVFIADEIEGLENVSEWHRELSEENLG"
     gene            complement(1680..2141)
                     /locus_tag="KRL67_003840"
     CDS             complement(1680..2141)
                     /locus_tag="KRL67_003840"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HBH1524668.1"
                     /translation="MIYEGKLIGKDLKIGIINSRFNEFITSKLLSGAEDCLLRHDVSS
                     ENIEIVWVPGAFEIPLVAQKMAKSGKYDAIICLGCVIRGATSHYDYVCSEVSKGIAKV
                     SLDSELPVIFGIVTTENIEQAIERAGTKAGNKGYDCAMNALEMANLFKSLN"
BASE COUNT          758 a          263 c          384 g          836 t
ORIGIN      
        1 aatataatgg attttttcaa tggtatttta acgagtatta ataatgcagt agtatttgta
       61 aacaattact tatggtcgta tatcttaatt ataatgttag ttgtaattgg tatttacttt
      121 actattaaaa caaattttgt acaattcaga tattttatag aaatgtttag acttctgggt
      181 gatggtacag cgaataaaaa tgcaaaaaag gaaggaaaaa tctcttcatt ccaagcattt
      241 tgtataagta catcttcaag agttggaact ggaaatatag ctggaatagc aattgcagtg
      301 gtagcaggag gacctggtgc tgtattttgg atgtggctta tagcattaat tggttctgca
      361 tcaagctttg ttgagagtac attagctcag atttacaaag tgaaaaatgg tcaagctttt
      421 agaggtggac cagcgtatta tatggaacaa ggtctaaata aaaaatggat gggtgtttta
      481 ttctcaatac ttataactat atgttacggt tttgtattta atgcagttca agcaaatact
      541 gtttcacttg catttaataa tgcatttgga ataagtaaaa tgactatggg aataggtctt
      601 ggaattttaa ctgcaatagt tatttttggt ggagtgcata gagttgcaaa agtttctgaa
      661 ataatagttc ctgtatttgc aggattatat atattagttg ctttggctat agttgtaatg
      721 aatataacag agataccaag tgttatagca ttgatagttg aaagtgcatt tgattttaaa
      781 ggaatggcta ttggtacatt tatgggagtt gtaatgactg gagtgaagag aggtctattt
      841 tctaatgaag caggtatggg tagtgctcct aatgctgctg caacagcaca tgtaactcat
      901 cctgtaaaac aaggtttaat tcaatcttta ggtgttttta cagatactat aataatatgt
      961 agttgtacag cattcatggt attattatat tcaggttata gtagtagtgg agcaactgga
     1021 attgaattag ctcaagaagc tttaactcat cacataggac cagttggaaa tatatttata
     1081 gcagtttgta tattcttgtt tgcatttagt tctatagttg gtaattacta ttatggtgaa
     1141 tcaaatatgg agtttatgag tggaagtaag actaaactta atgtatttag agtttttgtt
     1201 gtaggtatgg ttttatttgg ttcacttact caagtagatg tagtttggaa tcttgcagac
     1261 ttatttatgg cattgatggc gataataaac ttaatagcaa tagcactttt aggaaaatat
     1321 gcatttattg ctttaaaaga ttatacaagt caaaaaaaat caggaataaa agaccctgtg
     1381 tttattgcag atgaaataga aggtctggaa aatgtatcag agtggcatag ggaattatct
     1441 gaggaaaatt taggataaaa aaatagtata aaattacaaa atcattttga aagactagag
     1501 tatgtatata ctttatatga atataatatt gaattaattt atattactgg aatttttaat
     1561 tccagtaata tttttttatt atataattta gttttattaa aagtttttat tattaaaaat
     1621 agcttattta tcgtaaagta aatgatattg gcgtttttaa ttattatgaa tgttggtatt
     1681 taatttagag acttaaataa atttgccatt tctagtgcat tcatagcaca atcataacct
     1741 ttatttccag cttttgtacc tgctctttca atagcttgtt caatattttc tgtagttacg
     1801 attccaaata taacaggtaa ttcactgtct aaagatactt ttgctatccc tttagagact
     1861 tcgctacata cataatcata atgagaagta gctcctctta taacacagcc taaacagatt
     1921 atagcatcat acttaccact tttagccatt ttttgagcaa caagaggaat ctcaaaagcc
     1981 ccaggtaccc aaactatttc tatattttct gaagaaacgt cgtgtcttaa aagacagtct
     2041 tcagctcctg ataaaagttt tgatgttata aattcattaa aacgagaatt tattatacct
     2101 atttttaaat cttttccaat aagttttcct tcatatatca ttttatttcc tccaatttta
     2161 tttatgttat agatttatat tttaaatatt tttaatttta attataaaaa actacttagt
     2221 agaaaaatat catcatataa t
//
LOCUS       DAEPXF010000325         2152 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain HB27
            SAMN13376389-rid12479423.denovo.333, whole genome shotgun sequence.
ACCESSION   DAEPXF010000325 DAEPXF010000000
VERSION     DAEPXF010000325.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13376389
            Sequence Read Archive: SRR10518432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2152)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2152)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001069624.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001069624.1
            Genome Coverage       :: 315x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/15/2021 01:54:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,165
            CDSs (total)                      :: 4,076
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 89
            rRNAs                             :: 10, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 10 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 19 of 69
            Pseudo Genes (incomplete)         :: 44 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 10 of 69
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2152
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13376389-rid12479423.denovo.333"
                     /strain="HB27"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="China: Hebei"
                     /collection_date="2017"
     gene            127..2151
                     /locus_tag="KRL67_003841"
     CDS             127..2151
                     /locus_tag="KRL67_003841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009899983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBH1524669.1"
                     /translation="MDKKKLILITYDKLNSDHYKEELTNFFGDEIIIETQNILDGIKE
                     NLEGDVVLSLSPLTSNFLIKHFKEDIEIIHGTKALSKLGYEKMMKLPPGTKSLLMTTN
                     KTSAFEMATYLYKIGINHIDFVPTYPDCDEIYDLDTAITPGQIRFIPKYIKNIVDLGW
                     RKISLDTYMSLLVVLKLKNEKFIEKLYKLSKETLSHDFLNTSLDNISKLKTILYMTID
                     EIGDGLIFFNTFNKVTFVNKSLLNMLELDEKLIKSPSLMEYMPKSFLDKITKNLNIDN
                     MIIYIDEIDKKFILSKKPFYLYKNIEGCLITLKDVNNIEILEQKIRSDSVKRGYVAKY
                     KFNNIIGNSSIIKDCIKKAKKMALTDNPILITGETGTGKEAFTQSIHNHSNRKNKPFV
                     AINCASLPSELLESELFGYEDGSFTGAKKGGKKGLFELAHTGTIFLDEIGDMPHDLQV
                     KLLRVLQEKEIRKIGGTSIIPIDVRILAATNKDLEKLIIENKFRMDLFYRISMFTLDL
                     PPLRNRLEDIPLLLESFLKELPYKNIKLDKNLLEALNSYTWMGNIRELRNCIEYMAYM
                     GSNYLTINDLPQNISSKLNNNHMSSNVSIFNDLSQYDKNICISILKSLHMKPMGRTKL
                     MKFMEYNVTEYEVRNMLEYLTHNGYLISNKGRKGSSITEKGIKIIENNII"
BASE COUNT          926 a          249 c          292 g          685 t
ORIGIN      
        1 aaaatcctaa aaaaacctat tatttaatca aatatataaa tttatactgc tatttgttat
       61 ataatataac ttaccttata ctttaaatac ttcaaaaata aatactattt tgttaggaga
      121 aataaaatgg acaaaaagaa gttaatcctt ataacatatg ataaattgaa tagtgaccac
      181 tacaaagaag aattaacaaa cttttttgga gacgaaataa tcatagaaac acaaaatata
      241 cttgatggaa taaaagaaaa tttagaggga gatgtagtgc tttctctatc acctttaact
      301 agtaattttt taataaaaca ttttaaggaa gatattgaaa ttattcatgg aacgaaagca
      361 ctttcaaaac ttggatatga aaaaatgatg aaactacctc caggcacaaa atcgttactt
      421 atgacaacca ataaaacttc agcatttgaa atggctactt atctatataa aattggtata
      481 aatcatatag attttgtacc tacatatcca gattgtgatg aaatatatga tttagataca
      541 gctataacac caggtcagat tagatttata ccaaaataca taaaaaatat tgttgattta
      601 ggttggagaa aaatttcatt agatacatat atgtcattat tagtagtatt aaaactaaaa
      661 aatgagaaat ttatagaaaa actatacaaa ttgtccaagg agacactaag tcatgatttc
      721 ttaaatacat cattagataa tatatctaaa ttaaaaacta tactttacat gaccattgat
      781 gaaataggtg atggcctaat atttttcaat acctttaaca aggttacttt tgtaaataag
      841 tctttactta atatgctgga actagatgaa aaacttataa aatcaccttc tttaatggaa
      901 tatatgccaa agagtttcct agataaaatt acaaagaatt taaatattga taatatgatt
      961 atatacatag acgagataga taaaaaattc atactttcca aaaaaccatt ttatctttat
     1021 aaaaatatag agggttgctt aattacatta aaagatgtaa acaatataga aatattagaa
     1081 caaaaaataa gaagtgattc tgttaaaaga ggctatgtag ctaaatataa atttaataat
     1141 attattggaa atagttctat tataaaagat tgtattaaaa aggcaaaaaa aatggctctt
     1201 acagataatc ctatattaat aacaggtgaa acaggaacag gtaaagaagc ctttactcaa
     1261 tcaatacaca atcattcaaa tagaaaaaat aaaccttttg tagcaataaa ttgtgcttca
     1321 ttaccaagtg aattacttga aagtgaacta tttggatatg aagatggttc ttttactgga
     1381 gcaaaaaaag gtggaaaaaa aggattattc gaattagctc atactggcac tatattttta
     1441 gatgagatag gagatatgcc acatgattta caggttaaac ttttaagagt cttacaagaa
     1501 aaagaaatta gaaaaattgg tggtacaagt atcattccaa ttgatgtgag aatattagct
     1561 gccacaaata aagatttaga aaaattaatt atagaaaata aatttaggat ggatttattt
     1621 tataggataa gtatgttcac tttagattta cctcctctta gaaatagatt agaagatata
     1681 cctcttcttt tagaatcatt tttaaaagaa cttccctata aaaacataaa gcttgataag
     1741 aatcttttag aagctttaaa ttcatatact tggatgggga atattagaga attgagaaat
     1801 tgtattgaat atatggctta catgggttct aattatctta caataaatga tttacctcaa
     1861 aacatatctt caaaattaaa taataatcat atgtcaagta atgtgtccat attcaatgat
     1921 ttaagtcaat atgacaaaaa catatgcatt tctatcctaa aatcattgca catgaaacca
     1981 atgggaagga caaaattaat gaaatttatg gaatataatg tcacagaata tgaagtaaga
     2041 aatatgctag aatatttgac acacaatgga tacttaattt ctaataaagg aagaaaaggc
     2101 tcatcaataa ctgagaaagg cataaaaata atagaaaata atataattta aa
//
LOCUS       DAEPXF010000326         2144 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain HB27
            SAMN13376389-rid12479423.denovo.334, whole genome shotgun sequence.
ACCESSION   DAEPXF010000326 DAEPXF010000000
VERSION     DAEPXF010000326.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13376389
            Sequence Read Archive: SRR10518432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2144)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2144)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001069624.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001069624.1
            Genome Coverage       :: 315x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/15/2021 01:54:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,165
            CDSs (total)                      :: 4,076
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 89
            rRNAs                             :: 10, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 10 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 19 of 69
            Pseudo Genes (incomplete)         :: 44 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 10 of 69
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2144
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13376389-rid12479423.denovo.334"
                     /strain="HB27"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="China: Hebei"
                     /collection_date="2017"
     gene            185..466
                     /locus_tag="KRL67_003842"
     CDS             185..466
                     /locus_tag="KRL67_003842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH1524670.1"
                     /translation="MPKIEKDLDGTTTITYLNDDVDVPTDENGYKISMTILHGKTICH
                     VVSPETILGRKFTLEEKQEKDTIIYGAIEKIMVDRQLQIKNSNKEVASH"
     gene            613..1461
                     /locus_tag="KRL67_003843"
     CDS             613..1461
                     /locus_tag="KRL67_003843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003442793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="HBH1524671.1"
                     /translation="MIKSFLMLGQSNMAGRGFISEVTPIYNERIQMLRNGRWQMMTEP
                     INYDRPVSGVSLAASFADAWCCENQEDRIGLIPCAEGGSSLDEWNIDGILFKHAISEA
                     KFAIQSSELTGILWHQGENDSNNSNYKFYYKKLLSIIEALRKELNVPDIPIIIGGLGD
                     FLGKVGFGKSCTEYVFINQELQKFAFEQDNCYFVTATGLTSNPDGIHIDAISQRKFGL
                     RYFEAFHKKKHIMEALANESELIIPSNSKTYTKTEKIYINSMDFALGKISYDEFESKL
                     IKLNDN"
     gene            1513..2034
                     /locus_tag="KRL67_003844"
     CDS             1513..2034
                     /locus_tag="KRL67_003844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003442791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBH1524672.1"
                     /translation="MIPLLILGLLKQNPNSYGYELLALMEKKHYKYIVNFTKGSFYYN
                     LQQLEEKKFIEKVENKQSNKNRETHNYIITELGCQEFEKLMYKYGSKTEYINLSFYAT
                     LLFANDYKNEKLIELLETQIKQTQVRISLLNESIENEKTNSYYFNRMLENSRSHHLVN
                     IEWFEKLLNDIKD"
BASE COUNT          882 a          228 c          339 g          695 t
ORIGIN      
        1 atttattatg aaatttttat aatattttag cttagatatt taattatata taacatttta
       61 ttcatagtta actatttttg aagtttacct ttaatcaagc aacacttagt tactcaatta
      121 taaataatga ttgagaatag gacaattttt aagatacata aaatataaag aggaggggat
      181 ttaaatgcca aagatagaaa aagacttgga tggtacaact actataacat acctcaacga
      241 tgatgtagat gttccaacag atgagaatgg ttataaaata tctatgacaa tactacatgg
      301 aaagacaatc tgccatgtag tatctcctga aacaatctta ggaagaaagt tcacactaga
      361 agaaaaacaa gaaaaagata caataatata tggagcaata gagaaaataa tggtagacag
      421 acaattacaa ataaaaaatt caaacaaaga agttgctagt cattagcact tctttaataa
      481 gtgaactagg acaagtattt tttataatat tattgactta tagtctaatt agactataat
      541 ataaacatag atagtctaat tagactacta ttaaaaataa atacaataag aatttaagaa
      601 aggataataa caatgattaa atcattttta atgttagggc agtccaatat ggctggaaga
      661 ggattcataa gtgaagtgac tccaatttat aatgaaagaa tacagatgtt acgtaatgga
      721 agatggcaaa tgatgacaga acctattaat tatgatagac ccgtttctgg ggtaagtctt
      781 gctgcatcat ttgcagatgc atggtgttgt gaaaatcaag aggatagaat tgggctaatt
      841 ccttgtgctg agggaggtag ttccttagat gaatggaata tagatggtat actttttaaa
      901 catgctataa gtgaagctaa atttgccata caaagcagtg aattgacagg aattttatgg
      961 catcagggag aaaatgacag taataatagc aactataaat tttattataa aaaattactt
     1021 tctattattg aagctcttag aaaagaatta aatgttccag atataccaat tattattggt
     1081 ggattgggag acttcttagg aaaagtaggt tttggaaaaa gttgtactga gtacgtattt
     1141 atcaatcaag agttacagaa atttgctttt gaacaagata attgttactt tgttacagca
     1201 acaggtctta catctaatcc tgatggtatt catattgatg caatatctca aagaaaattt
     1261 ggattacgtt attttgaagc ttttcataag aaaaagcata ttatggaagc attagctaat
     1321 gaaagtgagt tgataatacc gagtaattcc aagacatata caaaaacaga aaaaatatat
     1381 ataaatagta tggactttgc actaggaaag atatcatatg atgagtttga atctaaactt
     1441 attaaactta atgataatta gataaaatat tcattaatat actagtaaat atagattgaa
     1501 agaggtgatt ttttgatacc cttattaatt ttaggattat taaaacaaaa tccaaattct
     1561 tatggatatg agttattagc attaatggaa aagaagcatt acaagtacat agtaaatttc
     1621 actaaaggtt ctttttatta taatttacag caacttgaag aaaaaaagtt tattgaaaaa
     1681 gttgaaaata aacaatctaa taaaaataga gaaactcata actacataat tactgaactt
     1741 ggatgtcagg aatttgaaaa actaatgtat aagtatggtt ctaaaacaga gtatataaat
     1801 ctatcatttt acgcaacact attatttgcc aatgattata aaaatgaaaa gttaatagaa
     1861 ttattggaaa cgcaaattaa gcaaactcaa gtaagaattt ctctattaaa tgaatccata
     1921 gaaaatgaaa aaacaaattc atattatttt aataggatgc tagaaaattc tcgttctcac
     1981 catttagtta atattgaatg gtttgagaag ttgctaaatg atattaaaga ttgattttaa
     2041 taataaaaaa atatacatat atactttata taaatgtatg atatatagat taaaaataag
     2101 gcagatgatt ttcaattgaa tatctgtctt tttgttatat aagg
//
LOCUS       DAEPXF010000327         2118 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain HB27
            SAMN13376389-rid12479423.denovo.335, whole genome shotgun sequence.
ACCESSION   DAEPXF010000327 DAEPXF010000000
VERSION     DAEPXF010000327.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13376389
            Sequence Read Archive: SRR10518432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2118)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2118)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001069624.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001069624.1
            Genome Coverage       :: 315x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/15/2021 01:54:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,165
            CDSs (total)                      :: 4,076
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 89
            rRNAs                             :: 10, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 10 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 19 of 69
            Pseudo Genes (incomplete)         :: 44 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 10 of 69
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2118
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13376389-rid12479423.denovo.335"
                     /strain="HB27"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="China: Hebei"
                     /collection_date="2017"
     gene            273..2057
                     /locus_tag="KRL67_003845"
                     /pseudo
     CDS             273..2057
                     /locus_tag="KRL67_003845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860844.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding repeat-containing protein"
BASE COUNT          919 a          279 c          371 g          549 t
ORIGIN      
        1 ttttatacaa aaccatgttt tttataacaa ttcattaaca taaaaatcaa acttacctta
       61 aaatataaat ttacaaattt ttcaatcaat aagaaaattt ctttattcct tacataatga
      121 ctgcaatgta acaaaataat tacaaattaa ttatgaaatc attatagtta gaaacctctt
      181 taaaattaga tattaagtat cctttagaat tagaacggga acaaagttaa aaaattaagt
      241 aatatcaaat ggacaagggg gaagaaaata aaatgaaaat accaaagaag attgctgcaa
      301 tgcttacagt tacaatgata gctggaagtt caacagcagg catagctagt gcacaaactg
      361 tggcaacaaa tttaacaggg caagaaagat atgaaactgc tgttaaaata agtcaagatg
      421 gatggaaaaa tgctgatgag gtagtaatcg taaacgactc atctatagca gatgctctat
      481 ctgcaacacc atttgcaaaa gcaaaaaatg ctccaatact tttaacaagt aaagataaat
      541 taaatgataa gacgaaagca gaaatacaaa gattaaaagc taaaaaagta tacttaatag
      601 gtggaacttc tgtattaagt acaaatatag aaaaagaaat aaaagattta aaaatatctt
      661 ttgagagaat ctctggagct gagagatatc aaacatcctt agaattggca aagaaattag
      721 atgctataag tgatgttaag aaaatagctg tagttaatgg tgaaaaagga cttgcagatg
      781 ctgtaagtgt aggagcacca gctgcacaaa acaatatgcc agtaatactt gctgattcta
      841 aaaatggaac agcagttgca gacaaattta taaaagatgc tggaataact caatcttatg
      901 tagtaggtgg agagagttca atttctgaag ctgttaaaaa taaactacca aatagtacaa
      961 gactaggtgg aacagataga aatgatacaa atgcaaaagt aataaaagaa ttttataaaa
     1021 agacagattt aaaaaatgct tatgtaacta aagatggaat gaataaacaa gaccaactta
     1081 tagatgcact agcagtaggt gtattaggtg ctaaaaatca atcaccagta gttttagttg
     1141 gaaagaacct atcagcttca caaaaatctt tagttaactc taagagtttt gataagataa
     1201 ctaaagttgg tggaaatggt aatgagacag catttaatga aatgaaatca ctacaagaag
     1261 taaaaacagt tgaagctaaa acaatttctg aattaaaatc agctatagat aaagctactg
     1321 ctaatgatgt tataaacttt aagccaacta gtgaggttaa agaagcattt acaatacaaa
     1381 ctgataaagc agtaacagta aacttaaatg gtacatatac aaagactgtg actatcaaca
     1441 tgcctaatgg tgatgtaaat aactatgcaa aagtggatga tgtagttata gatgatgtaa
     1501 aagatggaac ttttgtcaac tatggaaaaa taactaactt aaaggttaat gataaaaatg
     1561 gagctaagat agagaataat tctaaaggtg agattggtag tttaacagta gcatctggag
     1621 caagtcaagt aaaagtgact aatggtggta agataactac agttacaaat aattctaaag
     1681 ggactactat agataataaa ggtacaataa gtagtgttaa gggagataat tctccaacaa
     1741 taagtggaaa tagtccaagt tctaactctt caggaggaag ttcatcaagt ggaggttctt
     1801 cacatggagg aggctcatct tcaggaggaa gttcttctaa tcaaacatca gtaaataatg
     1861 aagcagcaaa aataacaagc gtacctacac cagcaaaaga tgcaactaaa ttaactatgc
     1921 catcagtatc aagtggatat agtatagcaa taaaaacatc aagtaatgag agcgttataa
     1981 aaaaagatgg aacaataata cctccaaata cagcaacaac agtaaaatta gtatttacag
     2041 taacacatac atcaagtggt aaaacagcag atacaaaaga actatctgta acagtaccag
     2101 ctaaatcaac agaagtac
//
LOCUS       DAEPXF010000328         2058 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain HB27
            SAMN13376389-rid12479423.denovo.336, whole genome shotgun sequence.
ACCESSION   DAEPXF010000328 DAEPXF010000000
VERSION     DAEPXF010000328.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13376389
            Sequence Read Archive: SRR10518432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2058)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2058)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001069624.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001069624.1
            Genome Coverage       :: 315x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/15/2021 01:54:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,165
            CDSs (total)                      :: 4,076
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 89
            rRNAs                             :: 10, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 10 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 19 of 69
            Pseudo Genes (incomplete)         :: 44 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 10 of 69
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2058
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13376389-rid12479423.denovo.336"
                     /strain="HB27"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="China: Hebei"
                     /collection_date="2017"
     gene            40..414
                     /locus_tag="KRL67_003846"
     CDS             40..414
                     /locus_tag="KRL67_003846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBH1524673.1"
                     /translation="MNIVISNSSGKPIYEQITTQIKSMIISGELPEGSALPSMRLLAK
                     ELRISVITTKRAYSDLERDGFIETVTGKGSFVSSKNMDFVKEEQFRLIEEYLQKAVDT
                     AKSSDITYDELKDILFLLYKGE"
     gene            411..1274
                     /locus_tag="KRL67_003847"
     CDS             411..1274
                     /locus_tag="KRL67_003847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBH1524674.1"
                     /translation="MISMDAITIKNLNKTYKNFSLQDISFSVPKGSVMGFVGENGAGK
                     TTTLKAILNLISYDSGSIEIFGLDSKKNEKEIKEQIGVVFEGSNFHENLNTDHVSKIM
                     SKIYKNWSDTLFKDYLKKLRVPDNKLIKEFSKGNKMKLSIAVALSHNPKLLILDEATS
                     SLDPIVREEILDIFLDFIQDEEHSIILSSHITSDLDKIADYITFIHKGKIVFSENKDE
                     LIDTMGVLKCKPSDFDNLSREDYSYYRKSQFGYEVLLKDKHRFISRHPNCIVDNTSIE
                     EIMLFYVRGDK"
     gene            1274..1912
                     /locus_tag="KRL67_003848"
     CDS             1274..1912
                     /locus_tag="KRL67_003848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 transporter permease"
                     /protein_id="HBH1524675.1"
                     /translation="MKGLILKDLLNLKGNVKFILLFIIMFGFMSSLGDGNVNNFIGVI
                     IVLCTTMIVSTFSYDDLNKWDSYVLTMPINRNDIVLSKYLTMLIFSFTGVLVSLIVSV
                     TIGYFKNTLILNETLLINALILSISVCFGSLILPLIYKFGTERARLSIILCFLVPTLA
                     LLVFKSILENISSPISIEIILNTLVYSLPFVAILLFVISYFISSKIYSKKEV"
BASE COUNT          774 a          221 c          310 g          753 t
ORIGIN      
        1 tgtgtatata ctatatatac aatttaagga ggtgtcttat tgaatatagt tattagtaat
       61 tcgagtggga agccaatata tgaacagatt actacacaaa ttaaaagtat gattataagt
      121 ggagagttac cagaaggctc agctcttcca agcatgagac tattggcaaa ggaacttaga
      181 ataagtgtaa ttactacaaa acgagcatat agcgatttag aacgagacgg atttattgag
      241 acagttactg gtaaaggcag ttttgtatct agtaaaaaca tggattttgt caaagaagaa
      301 caatttcgtt taattgaaga atacctacaa aaagcagttg atacagcaaa aagtagtgat
      361 attacatatg acgaattaaa agatatttta tttttattat ataaaggaga atgataagta
      421 tggatgcaat aactataaaa aatttaaata aaacttataa aaattttagc ctacaagata
      481 tatctttttc agtaccaaaa gggagtgtaa tgggatttgt aggtgaaaat ggcgcaggaa
      541 aaacaactac attaaaagca attttaaatt taatatctta tgatagtgga agtatagaaa
      601 tctttggtct tgatagtaaa aaaaatgaaa aagaaattaa agagcaaatt ggggttgtat
      661 ttgagggaag caactttcat gaaaatttaa acactgacca tgtttcaaaa atcatgagta
      721 aaatctataa aaattggagt gacacattat ttaaagacta cttaaaaaaa ttgagagtac
      781 cagacaataa attaattaag gagttttcaa agggtaataa gatgaaactt agtattgctg
      841 tagccttatc tcataaccca aaattattaa tactagatga ggcaactagt tcacttgacc
      901 ctatcgttcg tgaagaaata ctagatatat ttttagattt tatacaagat gaagaacact
      961 ctattatctt atcttcacat attacaagtg atttagataa gatagcagac tatattacat
     1021 ttatacataa aggaaaaatt gtatttagtg aaaataagga tgaattaatt gatactatgg
     1081 gagttttaaa atgtaaacct agtgattttg ataacctttc aagagaggat tattcatatt
     1141 atcgtaaaag ccaatttgga tatgaagtac ttcttaaaga taagcataga tttatctcaa
     1201 gacatccaaa ctgtattgtt gataatacgt ctatagaaga aattatgtta ttttatgtga
     1261 gaggtgataa ataatgaaag gtctaatttt aaaagattta cttaatttga aaggaaatgt
     1321 aaagtttata cttttattca tcattatgtt tggctttatg tcttctttag gagatggaaa
     1381 tgttaataac tttataggtg ttattatagt tttatgtact acaatgattg tttcaacatt
     1441 ttcatatgat gatttaaata aatgggatag ttatgtgctt acaatgccta ttaatcgcaa
     1501 tgatattgta ttaagtaaat atttaactat gttaatattt agcttcacag gtgttcttgt
     1561 ttcacttatt gttagtgtaa ctattggtta ttttaaaaat acacttatat taaatgaaac
     1621 tctacttatt aatgcactaa tcttatcaat ttcagtatgt tttggtagcc ttattctacc
     1681 acttatttat aaatttggta cagaaagagc tcgtttatca ataattttat gctttttagt
     1741 tccaacacta gctttattag tttttaaaag tattttagaa aatattagtt cacctatttc
     1801 tatagaaatt atacttaata cactagtata ttctcttcca tttgttgcta ttttactatt
     1861 tgtgatttcg tattttatat catcaaaaat ctatagtaaa aaagaagtat aaaatatact
     1921 attatatgtt tctgtattta agtatttaaa taaaatacta tcgagtttat tttggtagta
     1981 ttttattttt taattattaa tatctactag gcatttatta ttttattact tatatttaat
     2041 aacactatac actacttt
//
LOCUS       DAEPXF010000329         2040 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain HB27
            SAMN13376389-rid12479423.denovo.337, whole genome shotgun sequence.
ACCESSION   DAEPXF010000329 DAEPXF010000000
VERSION     DAEPXF010000329.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13376389
            Sequence Read Archive: SRR10518432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2040)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2040)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001069624.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001069624.1
            Genome Coverage       :: 315x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/15/2021 01:54:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,165
            CDSs (total)                      :: 4,076
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 89
            rRNAs                             :: 10, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 10 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 19 of 69
            Pseudo Genes (incomplete)         :: 44 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 10 of 69
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2040
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13376389-rid12479423.denovo.337"
                     /strain="HB27"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="China: Hebei"
                     /collection_date="2017"
     gene            37..1692
                     /locus_tag="KRL67_003849"
     CDS             37..1692
                     /locus_tag="KRL67_003849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH1524676.1"
                     /translation="MNLDFHYYGTYLAAKIAGYNDSDAKTIAYAAQYVDESSKNMILD
                     DVNFAFPTIQTNLEFEKYYADFTSLGYKWNLDSLNEIKKVWIPFHFLPGNLNNQVKYN
                     GVKESKGLTTNWKFKDGDDQKFRLMCLPNSETVSAIINDLIYFHSTEEYKLQFIGIRM
                     HVLADTWAHMYFIGKPEWYINDIKEFIAEESKYIEETKWTKAEEYRNSSWHSINFTGT
                     PDVGGYESISYLGHGRIGHLPDYGYLNYRYVPNWSCTDNKVKIQKNNQDNFFKAFCQM
                     VYALKCIKNGNDFSINQYDNLTAQQKTEVSKVIATRKDDQSEAWKKAIQILGYNSLEN
                     FDKNKWKNEFIKSTNKEKTDYYYFNLVAKKHVNYVIDFLEEKRLPLEEMCSKYCEFKT
                     FDDTQFAKNRNRVTKIILRGAYIVDAIQLVYDGKYKTPMHGETLGGSMVSLDLEADDY
                     IVKISGSMGLYEGGEPYPKSPTRTIGKITFLTKKGKTITTGCETMFKSYGNFTLEAPA
                     GKQIFALQGSYLIRKLRAETNKRYLDCLGIASIKDCSVVTSTK"
     gene            complement(1934..2026)
                     /locus_tag="KRL67_003850"
                     /pseudo
     CDS             complement(1934..2026)
                     /locus_tag="KRL67_003850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001990088.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
BASE COUNT          805 a          243 c          330 g          662 t
ORIGIN      
        1 taattatttt tttaaataaa attaagaggt gattttatga atttagattt tcactactat
       61 ggtacatatc ttgcagctaa aatagctggt tataatgatt ctgatgcaaa gacaattgcc
      121 tatgcagcac aatatgtaga cgaatcgagt aaaaatatga ttcttgatga tgtaaacttt
      181 gcatttccta caatacagac taatttagaa tttgaaaaat attatgcaga ttttacctca
      241 ttgggttata aatggaatct tgatagtttg aatgaaataa agaaagtatg gataccattt
      301 cattttttac ctggaaattt aaataatcaa gttaagtata atggagttaa agagagtaaa
      361 ggtttgacaa caaattggaa atttaaagat ggagatgacc aaaaatttag attgatgtgc
      421 ttacctaata gtgaaacggt ttctgctatt ataaatgatc ttatttattt tcatagtact
      481 gaagaatata aattacagtt tattggaata agaatgcatg tacttgcaga tacatgggca
      541 cacatgtatt ttattggaaa gccagaatgg tatattaatg atattaaaga atttatagca
      601 gaagaaagta aatacataga agaaactaaa tggaccaaag cagaagaata tcgtaatagc
      661 agttggcatt ctattaactt tactggtact cctgatgttg gcggatatga gagtatttca
      721 tatcttgggc atggaagaat tggtcatcta ccagattatg gatatcttaa ttatagatat
      781 gttccaaatt ggagttgtac tgataacaaa gtgaagatac aaaaaaataa tcaagataat
      841 ttctttaaag cattttgtca aatggtctat gcattaaaat gtattaagaa cggaaatgac
      901 tttagtataa atcaatatga taatttaact gctcaacaaa aaacagaggt ttcaaaagtc
      961 atagcaacaa gaaaagatga ccaatcagaa gcatggaaaa aagcaataca aattttaggg
     1021 tataactcct tagaaaattt tgataaaaac aagtggaaaa atgaatttat taaatctact
     1081 aataaagaaa aaacagacta ctattatttt aatctagtag ctaagaaaca tgtaaattat
     1141 gtaattgact ttttagagga aaagagacta ccattagaag aaatgtgtag taaatattgt
     1201 gaatttaaaa cttttgatga tacacaattt gctaaaaata gaaatagagt aacaaagatt
     1261 atacttagag gagcttatat agttgatgct atacaacttg tatatgacgg aaaatataaa
     1321 actccaatgc atggtgaaac attaggaggc tcaatggtat ctcttgattt agaagctgat
     1381 gattacatag ttaagataag cgggtctatg ggcttatatg aaggtggaga gccctaccct
     1441 aaatcaccaa ctagaacaat aggaaaaata acttttttaa ctaaaaaagg aaaaacaata
     1501 acaacagggt gtgaaactat gtttaaatca tatggaaatt ttactttaga agcacctgcc
     1561 ggaaaacaaa tttttgcact tcaaggttca tatttaatac gtaaattaag agcagagact
     1621 aacaaacgat atttggattg tttaggaatt gcaagcataa aagattgttc agtagttaca
     1681 agtacgaaat aaatacatat tggatagcta atgttataat ttatttaaga aaatggcaca
     1741 gtattttatg tttatattta cattaagagc actcttttta tatgagtgct tatttttttg
     1801 aaattcatta gtatataaac tatcaagaac gttactcaat ataccttatt tacttcacca
     1861 atgactatct tatatatact aacaataaaa aaagacccta aaaagagtct ttcccgaaaa
     1921 atctatatat atattataat atagttatat tttctgcttg aggacctcta gcacctttaa
     1981 ctatattaaa gcttacttgt tgaccttctt ctaatgattt aaatcctgaa gtttatatag
//
LOCUS       DAEPXF010000330         2001 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain HB27
            SAMN13376389-rid12479423.denovo.338, whole genome shotgun sequence.
ACCESSION   DAEPXF010000330 DAEPXF010000000
VERSION     DAEPXF010000330.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13376389
            Sequence Read Archive: SRR10518432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2001)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001069624.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001069624.1
            Genome Coverage       :: 315x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/15/2021 01:54:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,165
            CDSs (total)                      :: 4,076
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 89
            rRNAs                             :: 10, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 10 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 19 of 69
            Pseudo Genes (incomplete)         :: 44 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 10 of 69
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2001
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13376389-rid12479423.denovo.338"
                     /strain="HB27"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="China: Hebei"
                     /collection_date="2017"
     gene            <1..499
                     /locus_tag="KRL67_003851"
     CDS             <1..499
                     /locus_tag="KRL67_003851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="M18 family aminopeptidase"
                     /protein_id="HBH1524677.1"
                     /translation="EEVGSSTKQGADSNMLLNILERICISMGKDREGFFESIYSSFMI
                     SSDLAHAIHPNIPEKHDPTNKPIMGKGPVIKINASQAYTSDGYSIAIYKNICREANVE
                     YQEFVNRSDERGGSTIGPISSTHIDIPSVDVGSPILAMHSIRELGNVEDHYSIYKTFS
                     KFFEI"
     gene            530..1282
                     /locus_tag="KRL67_003852"
     CDS             530..1282
                     /locus_tag="KRL67_003852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HBH1524678.1"
                     /translation="MKLGLCLEGGGAKGAYQAGVVKALYDGGINKFYSISGTSIGAIN
                     GYYLYTGNVDNLEKMWTNIKDIQNGNVKIVNNTVDNSPAIDNLRELDDSNIEEMNFYV
                     NYVEVDNKVVSEKIVDVSKMPRNEAIISISYSGLLPSNPNATLGFKEQFVKDVQEGIY
                     DGFKLDGGLIRSALIEPLIGDNVDKIILVSTKYNYELPEDIKKIYDEDKIIVVRPNTQ
                     FAPKDTLNFDDKFCKTIYQEGYEIGKNILDRL"
     gene            1347..1871
                     /locus_tag="KRL67_003853"
     CDS             1347..1871
                     /locus_tag="KRL67_003853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HBH1524679.1"
                     /translation="MENKNPIVTIEMENGKEIKIELYPNIAPNTVKNFVSLVNEGYYN
                     GIIFHRVIPGFMIQGGCPNGTGMGGPGHSIKGEFSGNGFTNNLKHERGVISMARTMAP
                     NSAGSQFFIMHKNSPHLDGQYAGFGRVIEGMDTVDEIASVRTDSADKPQTPQIMKSVT
                     VETFGIDYSDVEKN"
BASE COUNT          800 a          213 c          377 g          611 t
ORIGIN      
        1 tgaagaagtt ggtagctcta ctaaacaagg tgcagattca aacatgttgt taaatatatt
       61 ggagagaatt tgcatatcaa tgggtaagga tagagaggga ttctttgagt ctatttattc
      121 atcttttatg atatcatcag atttagctca tgctattcat ccaaacattc cagaaaagca
      181 tgacccaact aataaaccaa taatgggaaa aggtccggtt ataaagataa atgctagtca
      241 ggcatatact agtgatggat attctatagc tatttataaa aatatatgta gagaagctaa
      301 tgttgagtat caagagtttg ttaataggtc agatgaaaga ggaggaagta ctataggtcc
      361 aatatcttct actcatatag atataccatc tgtggatgtg ggaagtccaa ttttagcaat
      421 gcactctata agagaattag gaaatgttga ggaccactat agtatatata aaacatttag
      481 taagtttttt gaaatataaa tataagataa acaatttgga gatgagacta tgaaattagg
      541 attatgttta gaaggtggag gagcaaaagg ggcttatcaa gcaggagtag ttaaagcctt
      601 atatgatgga ggaataaata aattttattc gatttcaggg acatcaatag gagcaataaa
      661 tggatattat ttgtacacag gaaatgttga taacttggag aaaatgtgga caaatataaa
      721 agatatccag aatggaaatg taaaaatagt aaataatact gtggataatt ctcctgccat
      781 agacaaccta agggaactag atgatagtaa tatagaagaa atgaactttt atgttaatta
      841 tgttgaagtt gataataaag tagtatcaga aaagattgtt gatgtatcaa aaatgcctcg
      901 taatgaagca attatcagca tttcatacag tgggttgtta ccatcaaatc caaatgcaac
      961 tcttggattt aaagaacaat ttgtgaaaga tgtacaagaa ggaatttatg atggtttcaa
     1021 attagatgga ggtttaatta ggagtgcatt aattgaacca ctaattgggg ataatgtgga
     1081 taaaattatt ttagtatcca caaaatataa ttatgagttg ccagaggata ttaaaaaaat
     1141 atatgatgaa gataaaataa tagtagttag acctaatact caatttgctc ctaaagatac
     1201 gcttaacttt gatgataagt tttgtaaaac tatttatcaa gaaggatacg aaataggaaa
     1261 aaatatactt gatagacttt aatttagagt ataatataat aaatgtaatt atgtaattta
     1321 actaatgaaa aggaaggtaa taaattatgg aaaataaaaa tcctatagta actatagaaa
     1381 tggaaaatgg aaaagaaatc aaaattgagt tatatccaaa tatagctcca aatacagtta
     1441 aaaactttgt atcattagta aatgaaggat attacaatgg aataatattt catagagtaa
     1501 taccaggatt tatgatacaa ggaggatgcc caaatggtac aggaatgggt ggtccaggtc
     1561 actcaataaa aggtgaattt agtggtaatg gatttactaa caatttaaaa catgaaagag
     1621 gagtcatatc aatggctaga actatggcac caaattcagc tggttctcaa tttttcataa
     1681 tgcataaaaa ttcaccacac ttagatggtc agtatgcagg ttttggtaga gtaatagaag
     1741 gtatggatac agttgatgaa atagcatcag ttagaacaga ttcagctgat aagccacaaa
     1801 ctccacaaat tatgaagtct gttactgtag aaacttttgg aatagattac tcagatgtag
     1861 aaaaaaacta aaacttatat aaaaactagg ttgagttata aataaaaatt aaagatatat
     1921 attacttaat aatgaggata aagtgaaatg ttagtatgtt tttagacaaa tacatttcac
     1981 tttttaattt atttgtattg t
//
LOCUS       DAEPXF010000331         2000 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain HB27
            SAMN13376389-rid12479423.denovo.339, whole genome shotgun sequence.
ACCESSION   DAEPXF010000331 DAEPXF010000000
VERSION     DAEPXF010000331.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13376389
            Sequence Read Archive: SRR10518432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2000)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2000)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001069624.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001069624.1
            Genome Coverage       :: 315x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/15/2021 01:54:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,165
            CDSs (total)                      :: 4,076
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 89
            rRNAs                             :: 10, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 10 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 19 of 69
            Pseudo Genes (incomplete)         :: 44 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 10 of 69
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2000
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13376389-rid12479423.denovo.339"
                     /strain="HB27"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="China: Hebei"
                     /collection_date="2017"
     gene            138..1451
                     /locus_tag="KRL67_003854"
     CDS             138..1451
                     /locus_tag="KRL67_003854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3440 domain-containing protein"
                     /protein_id="HBH1524680.1"
                     /translation="MDKRYLESDVFEAFQERLSYLFNEFDYIYVSFSGGKDSGLLLNL
                     VLDYKRKNNISSKIGVFHQDFEAQYSLTTEYVEKVFEENIEDIEPYWVCLPMATRTAV
                     GSYEMYWYPWDDEKEELWVREMPVKEYIINQKNNPFTYYKYKMRQESLAKQFGRWIRD
                     IHEGAKVVCLLGTRASESLQRYCSIVNKQHGYKGKCWITKEFKNVWTASPIYDWEIED
                     IWLAHYKFDYDYNKIYDLFYKAGVAPRKMRVASPFNDSAKESLNLYRILDPDVWVKLV
                     ARVQGVNFTSIYGKTKAMGYRSVKLPYGHTWKSFTHFLLDTLPENIRNSYIKKFSTSI
                     EFWQNTGGGLSEIVIRELLRKGYKIELNGVSNHTKDKKSRVIFLDDIPDDTDDIKFTK
                     DIPSWKRMCYCILKNDHMCRFMGFGPTKEQRIRTEYIKKKYEKIH"
     gene            1469..>2000
                     /locus_tag="KRL67_003855"
     CDS             1469..>2000
                     /locus_tag="KRL67_003855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB-like nuclease domain-containing protein"
                     /protein_id="HBH1524681.1"
                     /translation="MEYKSPVYGIIAVPIEKIESNDYNPNAVAPPEMQLLYKSIKEDG
                     YTMPIVCYYNEEKDKYIIVDGFHRYRVMLENEDIYDRENGLLPVSVIDKSLDERMAST
                     IRHNRARGSHNVDLMSKIITELCEIGKSDEWISKHLGMEVDEILRLKQITGLAYLFKD
                     KEFGNAWIPFGSDIEEE"
BASE COUNT          762 a          203 c          395 g          640 t
ORIGIN      
        1 ttacattata attatatgta tttagttgga tttgtgtatt taaataatgt ataatttatg
       61 ctgattactg ctaaaaaagc ataataatat tgtattgtaa ggttttataa acttctaatt
      121 gaaaggggat aattcaaatg gataagagat atttagaatc cgatgtattt gaagcttttc
      181 aagaaagact ttcttatctt tttaatgaat ttgattatat ctatgtatct tttagtggag
      241 gaaaagatag tggcttactg ctaaatttag ttttggatta taagagaaag aataatataa
      301 gttccaaaat aggagtattt catcaagatt ttgaggctca atatagtttg actactgaat
      361 atgttgaaaa agtatttgaa gagaacattg aagatattga accatattgg gtgtgtttac
      421 caatggctac aagaactgct gtaggaagtt atgaaatgta ttggtatcca tgggatgatg
      481 agaaagaaga attatgggta agagaaatgc cagtcaaaga atacataata aatcaaaaaa
      541 ataatccttt tacttattat aaatataaaa tgagacagga aagtttggca aaacaatttg
      601 gtagatggat tagagatata catgaaggtg caaaagttgt ttgcttatta ggaacacgag
      661 catcagaatc tctacaacgt tattgttcta ttgtgaataa acagcatgga tataaaggaa
      721 agtgctggat aactaaggaa tttaaaaatg tttggacggc atctcctata tatgattggg
      781 aaatagaaga tatatggcta gcccactata aatttgatta tgactataat aaaatttatg
      841 acttattcta taaagctgga gtagctccaa gaaaaatgag agtagcatca ccttttaatg
      901 attcagcaaa agagagttta aatttataca gaatattgga cccagatgta tgggtgaaat
      961 tggttgctag agtacaagga gttaatttta cttctattta tggaaaaaca aaggctatgg
     1021 gatatcgtag tgttaaattg ccctatggac acacatggaa aagttttaca cattttttgc
     1081 ttgatacctt acctgaaaat ataaggaata gctatattaa aaaattttca acatctattg
     1141 agttttggca gaatacaggt ggtggtttgt cagaaatagt tatacgtgaa ttgttgcgaa
     1201 aaggttataa aatagaatta aatggagttt caaatcatac aaaagataaa aagagtagag
     1261 tcatattttt ggatgatata cctgatgata cagatgatat taaatttacc aaagatatac
     1321 caagttggaa aagaatgtgt tattgtattt taaaaaatga ccatatgtgt agatttatgg
     1381 gatttggtcc tacaaaagaa cagagaatta gaacggaata tattaagaag aaatatgaaa
     1441 aaattcatta gataggagtt aagatgctat ggaatataaa agtcctgtat atggaatcat
     1501 cgcagtgcca atcgaaaaga tagaatctaa cgactataat ccaaatgcag tagccccacc
     1561 agaaatgcag ttgttgtata aaagcataaa agaagatggg tatacaatgc caattgtctg
     1621 ttattataat gaggaaaagg ataagtatat tattgtagat ggttttcatc gatatagagt
     1681 aatgcttgaa aatgaggata tatatgatag agaaaatgga cttcttcctg tttcagtaat
     1741 tgataaatct cttgatgaaa gaatggcaag tactattcga cacaatcgtg caagaggttc
     1801 tcataatgtg gatttaatga gtaaaattat aactgaactt tgtgaaattg gaaaatctga
     1861 tgaatggata tcaaagcacc tgggtatgga agttgatgaa attcttagat tgaagcaaat
     1921 aactggatta gcttatttat ttaaagataa agagtttggt aatgcatgga ttccttttgg
     1981 ctctgatata gaagaagaaa
//
LOCUS       DAEPXF010000332         1978 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain HB27
            SAMN13376389-rid12479423.denovo.340, whole genome shotgun sequence.
ACCESSION   DAEPXF010000332 DAEPXF010000000
VERSION     DAEPXF010000332.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13376389
            Sequence Read Archive: SRR10518432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1978)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1978)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001069624.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001069624.1
            Genome Coverage       :: 315x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/15/2021 01:54:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,165
            CDSs (total)                      :: 4,076
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 89
            rRNAs                             :: 10, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 10 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 19 of 69
            Pseudo Genes (incomplete)         :: 44 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 10 of 69
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1978
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13376389-rid12479423.denovo.340"
                     /strain="HB27"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="China: Hebei"
                     /collection_date="2017"
     gene            complement(111..662)
                     /locus_tag="KRL67_003856"
     CDS             complement(111..662)
                     /locus_tag="KRL67_003856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan transporter"
                     /protein_id="HBH1524682.1"
                     /translation="MKTNTKKLTLNAILLAMGLLIHQITPAIGLPMKPDVPLAMLFVI
                     LVLNRDDYKTCLIAGIVTGIFTALTSSFPGGQIPNVIDKTLTANIVFLLMHISYKMPF
                     IKKLAKKTQDTVVVAIIMPIGTLVSGTIFLLAAQAIVGLPGASFTALFLAVVLPAVLI
                     NLVAGIFLFKIVSLSIRRVSYQA"
     misc_binding    complement(814..1077)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(1513..1911)
                     /locus_tag="KRL67_003857"
     CDS             complement(1513..1911)
                     /locus_tag="KRL67_003857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH1524683.1"
                     /translation="MNKKKKLIGIAISFLLIISIINPLQVEAEAIIESSVYISSIKEN
                     YTYDNTKFKTIKSKSRHKSRKYNKSKVKLKKRSIRTKKYGYQTINKYKRRNARRHNIL
                     TKLILSIIVIFFVIVLVVLIFIKRKKKKFY"
BASE COUNT          660 a          295 c          191 g          832 t
ORIGIN      
        1 atataatacc aaatctttag acaacataag aataaaatag atataatcta ctttattctt
       61 atgttattta gttattataa tggtttataa agtaaaatat tatttcgtat ttaagcttga
      121 taactaactc ttcttattga tagactaaca atcttgaata aaaatattcc tgcaactaga
      181 tttattaaaa cagctggtaa tactactgct aagaataaag ctgtgaatga agcccctgga
      241 agaccaacta tagcttgagc tgctaataaa aatatagttc cacttacaag agttcctatt
      301 ggcattatta ttgcaactac tacagtatct tgtgttttct tagctaattt ttttatgaaa
      361 ggcatcttat aactaatgtg cattaataaa aatactatat ttgctgtaag agttttatct
      421 attacattag gaatttgacc tcctggaaaa cttgatgtaa gtgctgtaaa tattcctgtt
      481 actatacctg ctattaaaca agttttataa tcatctctat ttaaaaccaa tattacaaat
      541 aacattgcta gtggtacatc tggcttcatt ggtaatccta tagctggtgt gatttgatgt
      601 attaataatc ccatcgctaa aagtattgca tttaaagtta atttttttgt atttgttttc
      661 attttttgta cttcctccta tttttttaat tttcaattta tttttttgcc aacctactat
      721 atcatcatct ttagcttttg aaattcataa catctttctc accaccttct atcttttaaa
      781 ttttcagtaa aataaaaaaa ctccgtccta aactaatagg acggagatta tccgcgttgc
      841 cacctaaatt atataaaaat taatataaaa ttcaattttc atacatctca ttatcaggta
      901 cttacatacc ttattcgcta taacgtgcga ctgacgataa agtctactct caaaactatg
      961 atttcaattt attcctctaa gggccattca tcaaattttt tcttgctaag attccaccat
     1021 cctcagctct ctatactgaa cctaaattta actactcatc cttgtcatag gatttaattt
     1081 attgtattga attttgttat taaaataatg atattctttt ttctaaaaaa tgtcaacacc
     1141 tttttataaa aaatatattt tgtaatctat tttttataaa aaaagtaaat tatactatac
     1201 atttcttttc atatacaaaa ttctataaat aattacttta atgctctaat agtctgcttt
     1261 ttggctctaa ttcttcttct attaattttg tgaatatctg ttgacgtaat tttatatttt
     1321 gatgcatgag gaattaaatt ctttaaagtt aattaagtac atttatcaat aaatttactt
     1381 aatttgccat catttaatac tcatttctgt atttatatac ctcctttgaa acagaatatt
     1441 actccgtttt aattacatca tattattaaa tagataccaa tgattagaaa aatgatattc
     1501 ttttaattat ttttagtaaa attttttctt ctttctttta ataaatatta gtactactaa
     1561 aactataaca aaaaatatga ctattatgga caatattaat tttgttagga tattatgcct
     1621 tcttgcattt cgtcttttat atttatttat agtttgatat ccatactttt tagttcttat
     1681 acttcgtttt ttcaacttta ctttactttt attgtatttc ctacttttat gtctactttt
     1741 agatttaata gttttaaatt tagtattatc atatgtataa ttctctttta ttgaagaaat
     1801 gtatacacta gactcaataa tagcttctgc ttctacttgt aatggattta tgatgctgat
     1861 tataagtaga aaagatatag ctatcccaat caattttttc ttcttattca tattttcccc
     1921 ctcataaaaa tttttaacta gttcattata atatatatat aactaaaaat tacaaatt
//
LOCUS       DAEPXF010000333         1969 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain HB27
            SAMN13376389-rid12479423.denovo.341, whole genome shotgun sequence.
ACCESSION   DAEPXF010000333 DAEPXF010000000
VERSION     DAEPXF010000333.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13376389
            Sequence Read Archive: SRR10518432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1969)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1969)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001069624.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001069624.1
            Genome Coverage       :: 315x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/15/2021 01:54:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,165
            CDSs (total)                      :: 4,076
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 89
            rRNAs                             :: 10, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 10 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 19 of 69
            Pseudo Genes (incomplete)         :: 44 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 10 of 69
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1969
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13376389-rid12479423.denovo.341"
                     /strain="HB27"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="China: Hebei"
                     /collection_date="2017"
     gene            108..377
                     /locus_tag="KRL67_003858"
     CDS             108..377
                     /locus_tag="KRL67_003858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010963800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HBH1524684.1"
                     /translation="MKAILTVIGKDKPGIVAGISDELYKQNINIVDISQKILQDYFTM
                     IMIVDLEKSLNSFEKTVEDLVERGKKLSVDVKIQREEIFNSMHNL"
     gene            403..1758
                     /locus_tag="KRL67_003859"
     CDS             403..1758
                     /locus_tag="KRL67_003859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009170073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PFL family protein"
                     /protein_id="HBH1524685.1"
                     /translation="MNVGNILETIKMIDEEQLDIRTITMGISLLDCIDPDGDKAREKI
                     YNKIMSSAKDLVKVGKDIEREFGIPIVNKRVSVTPISIIAGATDENDYVKFAQTLDKA
                     AEDLGIDFIGGFSALVQKGYTKGDKILIKSIPKALASTNKVCASVNVGSTRCGINMDA
                     VKEMGEIIKETAELTKDAKGFGCAKLVVFCNAVEDNPFMAGAFHGVGEADRIINVGVS
                     GPGVVKRALEKVKGEPFDVVSETVKKTAFKITRVGQLVAKEASSRLDVPFGIIDLSLA
                     PTPAIGDSVANILEEMGLEVVGTHGTTAALALLNDAVKKGGVMACSHVGGLSGAFIPV
                     SEDAGMIDAVIKGALSIDKLEAMTAICSVGLDMIAVPGNTTAGTLGAMIADEAAIGMI
                     NNKTTAVRIIPAPGCDVGDMVEFGGLLGRAPVMPINTNSSELFTQRGGRIPAPIHSFK
                     N"
BASE COUNT          740 a          210 c          419 g          600 t
ORIGIN      
        1 tatatacata aaaagtggtt tactaacaaa taaatgaaat atatgttaat acatagttca
       61 atttgtaaaa atataagatt tataaaaaaa taagggagga tattattatg aaagcaattt
      121 taacagtaat tggaaaggat aaaccaggta tagtagcagg aattagtgat gaattataca
      181 agcaaaatat caacatagta gatatatcac aaaagatatt acaggattat tttactatga
      241 ttatgatagt tgatttagag aaatcattaa attcttttga gaaaacggtt gaggacttgg
      301 ttgaaagagg taagaaatta tcagtagatg taaaaattca gcgagaagaa atatttaatt
      361 caatgcataa tctgtagtag acagtaaggg gaggtgataa tagtgaatgt aggaaatata
      421 cttgaaacaa taaagatgat agatgaagaa cagttggata taagaactat aactatgggg
      481 atttctttat tagattgtat agaccctgat ggagataaag ctagagaaaa aatatacaat
      541 aagattatga gttctgctaa agatttggtg aaggttggaa aagatataga gcgtgaattt
      601 ggtatcccaa ttgtaaataa aagggtatca gtaactccaa tttccattat tgcaggtgca
      661 acagatgaaa atgactatgt aaagtttgcc cagacattgg ataaggctgc agaggattta
      721 ggtatagatt ttattggtgg attctcagca ttagttcaaa agggatatac aaaaggtgat
      781 aagatactaa ttaagtcaat tccaaaagct ctagcaagta ctaataaagt atgtgcttca
      841 gtaaatgtag gttctacaag atgtggtata aatatggatg cagtgaaaga aatgggagaa
      901 atcataaaag aaactgccga attaacaaaa gatgcaaaag gttttggttg tgcaaaatta
      961 gtagtatttt gtaatgcagt agaagataat ccatttatgg caggagcttt ccatggtgtt
     1021 ggtgaggctg atagaattat aaatgttggt gtaagtggac ctggtgttgt taagagagct
     1081 ttagagaaag taaagggaga gccctttgat gttgtttcag aaacagttaa aaagactgca
     1141 tttaaaatta caagagttgg acaacttgtg gcgaaggaag cttctagcag attggacgtt
     1201 ccatttggga taatagactt atcactagct cctacacctg ctattggtga ttcagtagca
     1261 aatatattag aggaaatggg tcttgaagtt gttggtactc atgggactac agcagcatta
     1321 gcacttttaa atgatgctgt taaaaaaggt ggagtaatgg catgttcaca tgttggagga
     1381 cttagtggag cgtttatacc agtatctgaa gatgcaggta tgattgatgc tgttattaaa
     1441 ggtgctttaa gtatagataa gcttgaagca atgactgcta tttgttctgt aggtcttgac
     1501 atgatagctg tgccaggaaa tactacagca ggtacgcttg gagctatgat agctgatgag
     1561 gcggcaatag gtatgattaa taataagact acagccgtta gaattatacc agccccagga
     1621 tgtgatgtag gtgatatggt agaatttgga ggacttttag gacgagcacc agtaatgcct
     1681 ataaatacaa attcatctga attatttact caaagaggag gaaggattcc agctcctata
     1741 cattcattta aaaattagtt atattacata taataatatt taactaaata tttagatggt
     1801 taaattaaaa aagtctatgc atttaaatat tatagttaag ataataaact ttgttaagat
     1861 aataaacttt aaaaatcaat tgttaagatt gtggaaaaaa taaagcgata catggacatt
     1921 taaaaattca tgtatcgctt ttattttaat aatatctaat taatataaa
//
LOCUS       DAEPXF010000334         1937 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain HB27
            SAMN13376389-rid12479423.denovo.342, whole genome shotgun sequence.
ACCESSION   DAEPXF010000334 DAEPXF010000000
VERSION     DAEPXF010000334.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13376389
            Sequence Read Archive: SRR10518432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1937)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1937)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001069624.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001069624.1
            Genome Coverage       :: 315x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/15/2021 01:54:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,165
            CDSs (total)                      :: 4,076
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 89
            rRNAs                             :: 10, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 10 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 19 of 69
            Pseudo Genes (incomplete)         :: 44 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 10 of 69
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1937
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13376389-rid12479423.denovo.342"
                     /strain="HB27"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="China: Hebei"
                     /collection_date="2017"
     gene            130..246
                     /gene="rrf"
                     /locus_tag="KRL67_003860"
     rRNA            130..246
                     /gene="rrf"
                     /locus_tag="KRL67_003860"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            253..327
                     /locus_tag="KRL67_003861"
     tRNA            253..327
                     /locus_tag="KRL67_003861"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:285..287,aa:Asn,seq:gtt)
     gene            332..406
                     /locus_tag="KRL67_003862"
     tRNA            332..406
                     /locus_tag="KRL67_003862"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:365..367,aa:Glu,seq:ttc)
     gene            412..487
                     /locus_tag="KRL67_003863"
     tRNA            412..487
                     /locus_tag="KRL67_003863"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:445..447,aa:Val,seq:tac)
     gene            493..569
                     /locus_tag="KRL67_003864"
     tRNA            493..569
                     /locus_tag="KRL67_003864"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:527..529,aa:Asp,seq:gtc)
     gene            581..655
                     /locus_tag="KRL67_003865"
     tRNA            581..655
                     /locus_tag="KRL67_003865"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:613..615,aa:Thr,seq:tgt)
     gene            670..754
                     /locus_tag="KRL67_003866"
     tRNA            670..754
                     /locus_tag="KRL67_003866"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:704..706,aa:Tyr,seq:gta)
     gene            764..837
                     /locus_tag="KRL67_003867"
     tRNA            764..837
                     /locus_tag="KRL67_003867"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:796..798,aa:Gly,seq:tcc)
     gene            848..924
                     /locus_tag="KRL67_003868"
     tRNA            848..924
                     /locus_tag="KRL67_003868"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:882..884,aa:Arg,seq:tct)
     gene            934..1009
                     /locus_tag="KRL67_003869"
     tRNA            934..1009
                     /locus_tag="KRL67_003869"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:967..969,aa:Gln,seq:ttg)
     gene            1021..1096
                     /locus_tag="KRL67_003870"
     tRNA            1021..1096
                     /locus_tag="KRL67_003870"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1054..1056,aa:Lys,seq:ttt)
     gene            1099..1187
                     /locus_tag="KRL67_003871"
     tRNA            1099..1187
                     /locus_tag="KRL67_003871"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1133..1135,aa:Ser,seq:tga)
     gene            1206..1296
                     /locus_tag="KRL67_003872"
     tRNA            1206..1296
                     /locus_tag="KRL67_003872"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1240..1242,aa:Ser,seq:gct)
     gene            1305..1381
                     /locus_tag="KRL67_003873"
     tRNA            1305..1381
                     /locus_tag="KRL67_003873"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1339..1341,aa:Pro,seq:tgg)
     gene            1467..1542
                     /locus_tag="KRL67_003874"
     tRNA            1467..1542
                     /locus_tag="KRL67_003874"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1500..1502,aa:Trp,seq:cca)
     gene            1603..1679
                     /locus_tag="KRL67_003875"
     tRNA            1603..1679
                     /locus_tag="KRL67_003875"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1637..1639,aa:Pro,seq:tgg)
     gene            1685..1761
                     /locus_tag="KRL67_003876"
     tRNA            1685..1761
                     /locus_tag="KRL67_003876"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1719..1721,aa:Ile,seq:gat)
     gene            1765..1840
                     /locus_tag="KRL67_003877"
     tRNA            1765..1840
                     /locus_tag="KRL67_003877"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1798..1800,aa:Phe,seq:gaa)
     gene            1848..1924
                     /locus_tag="KRL67_003878"
     tRNA            1848..1924
                     /locus_tag="KRL67_003878"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1882..1884,aa:Met,seq:cat)
BASE COUNT          490 a          443 c          523 g          481 t
ORIGIN      
        1 atttgatgtt cattcattaa gatatatgtg tagtttttag agtgttaact ctaaaaaaaa
       61 ataaaaaaag tattgacaaa aatagcaaaa aatgttaata ttatacttgt cctagcaaca
      121 agaaaaaaat gtggttataa tagcagagaa gatacacctg ttcccattcc gaacacagaa
      181 gttaagttct ctagcgctga tggtacttgg tgggaaactg cctgggagag taggacatag
      241 ccacgtgaat gttccaaggt agctcaacgg tggagcaacc ggctgttaac cggtaggctg
      301 tgggttcgag ccccaccctt ggagccatta tggctcgttg gtcaagaggt taagacaccg
      361 ccctttcacg gcggtaacag gggttcaatt cccctacgag tcaccatttg tgggatcata
      421 gctcagctgg gagagcacct gccttacaag cagggggtca caggttcgag ccctgttggt
      481 cccaccattt gcggcctggt agttcagttg gttagaatgc cagcctgtca cgctggaggt
      541 cgagggttcg agccccttcc aggtcgccaa aataaaaaat gctggtgtgg ctcaacggta
      601 gagcagctga cttgtaatca gcaggttgta ggttcgattc ctatcaccag ctccatgtaa
      661 aactaatacg gaggatttcc cgagcggcca aagggggcag actgtaaatc tgttgtcatc
      721 gacttcggtg gttcgaatcc accatcctcc accaattaaa tatgcgggtg tagctcaatg
      781 gtagagttct ggccttccaa gccagctgtg agggttcgat ccccttcacc cgctccaaag
      841 aaagcgtggg tgcatagctc agctggatag agcaacgccc ttctaaggcg tgtgtccggg
      901 gttcgaatcc ctgtgcgctc accacatata tattggggat tagccaagtc ggtaaggcac
      961 atgactttga ctcatgtatg cgtaggttcg agtcctgcat ccccagccaa ataaaaatat
     1021 ggtctattag ctcagtcggt agagcacctg acttttaatc agggtgtccc gcgttcgagt
     1081 cgcggataga tcaccaatgg agaggtgtcc gagtggttta aggagctggt cttgaaaacc
     1141 agtgacttcg aaaggggccg tgggttcgaa tcccaccctc tccgccaaaa aaaatgactg
     1201 aatttggaga agtactcaag tggctcaaga ggatcccctg ctaagggatt aggctgggta
     1261 accggtgcga gggttcgaat ccctccttct ccgccattca aaaacgaggt gtagcgcagt
     1321 ttggtagcgc acatggtttg ggaccatggg gccgggggtt cgagtccctt cacctcgacc
     1381 aacttaagtt tttagttcgg ttggttagag tgcccaactc ataactagta attctagggt
     1441 ttgagtccat gaaggttatt cttcataggg gtgtagctca gttggtagag cgtcggtctc
     1501 caaaaccgtg cgccgggggt tcgagtccct ccacccctgc caaataaaaa ggacttgaaa
     1561 aaagttggat tttagagtaa tataagatag taaataagat ttcgaggtgt agcgcagttt
     1621 ggtagcgcac atggtttggg accatggggc cgggggttcg agtcccttca cctcgaccaa
     1681 gaaagggcct atagctcagg tggttagagc gcacgcctga taagcgtgag gtcgctggtt
     1741 cgagtccagc taggcccacc atttgcccag atagctcagt cggtagagca ggggactgaa
     1801 aatccccgtg tcggtggttc gattccgcct ctgggcacca ggaatacggc ggtatagctt
     1861 agttggctag agcgttcggt tcatacccga aaggtcatag gttcgactcc tattaccgct
     1921 accatctaac tatatca
//
LOCUS       DAEPXF010000335         1935 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain HB27
            SAMN13376389-rid12479423.denovo.343, whole genome shotgun sequence.
ACCESSION   DAEPXF010000335 DAEPXF010000000
VERSION     DAEPXF010000335.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13376389
            Sequence Read Archive: SRR10518432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1935)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1935)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001069624.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001069624.1
            Genome Coverage       :: 315x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/15/2021 01:54:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,165
            CDSs (total)                      :: 4,076
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 89
            rRNAs                             :: 10, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 10 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 19 of 69
            Pseudo Genes (incomplete)         :: 44 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 10 of 69
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1935
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13376389-rid12479423.denovo.343"
                     /strain="HB27"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="China: Hebei"
                     /collection_date="2017"
     gene            172..1311
                     /locus_tag="KRL67_003879"
     CDS             172..1311
                     /locus_tag="KRL67_003879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HBH1524686.1"
                     /translation="MVIKLTIEEYKNKYFTGNKIVLSLNSFDNKELYYHETENILNLI
                     NEIDKLTSSKIFKTIEDKNDLALESYYSSVIEGAFSTRKIAKSIIRGKMKPSNKSEYM
                     IYNNHRALEYGLDNLDELYSHKFIYDLHHILGENCLDSEEYEYRTEKVYVCDSKGEII
                     HTGLEPLKIYDFMSKLIDFMENSKVSNLIKSAIIHFYFVYVHPFSDGNGRTSRALSYL
                     YLIDKGYDTFKEFSISYMISKNRTKYYKAILDVENKGNNLTVFIEFMLKSIIQSVNEM
                     RNMHDRKSLESILKEELFENDITLSATEEHILKYICNKDNYSMTLENYIKKNKSRYLK
                     AGIKEIELVDQLMEVFNNLEEIEILSKEKNIYKVNDKYLKMLDID"
     gene            1337..1525
                     /locus_tag="KRL67_003880"
     CDS             1337..1525
                     /locus_tag="KRL67_003880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH1524687.1"
                     /translation="MNNKKKAFLISGAINVISLIIVFSLFHYNIIENSTQNMIITVIL
                     IFIFEMIKIKIIEKYYKL"
BASE COUNT          820 a          161 c          278 g          676 t
ORIGIN      
        1 aaacttcctg taacatatat tttaaatgct tattcttaaa tttttgcatt atttattaaa
       61 atttattatg ctttaacgct aaattgagtt tgataattat aaaacaaata atcaaaaata
      121 gtgtaaaaat taatactttt tgttaatatt aatagataaa gataatctaa ggtggtgatt
      181 aaactgacaa tagaagaata caaaaacaaa tactttactg gaaataaaat cgtactttct
      241 ctaaatagtt ttgataataa agaactatac tatcatgaaa ctgaaaatat attaaattta
      301 ataaatgaaa tagataaatt aacatcaagt aagatattta aaacaataga agataaaaat
      361 gatttggcac tagagtctta ttattctagt gtaatagaag gtgctttttc aactagaaag
      421 attgcaaaat ctataattag aggaaagatg aaaccaagta ataaaagtga atatatgatt
      481 tacaataatc atagagcctt agaatatgga ttagataact tagatgaatt gtatagtcac
      541 aaatttatat atgatttgca tcatatattg ggcgaaaact gcttggattc agaagaatat
      601 gagtatagaa ctgaaaaagt atatgtatgt gattcaaaag gtgagattat acatacagga
      661 cttgaacctt tgaagatata tgactttatg agtaaattaa tagattttat ggaaaatagc
      721 aaagtaagta atttaattaa aagtgctata attcattttt actttgtata tgttcatcct
      781 tttagtgatg gtaatggaag aacatcaaga gccttatcat atctatattt gattgataaa
      841 ggatatgata ctttcaagga gttttcaata tcatatatga tatctaaaaa cagaactaag
      901 tattataagg ctatacttga tgtagaaaac aaaggaaata atttaactgt atttatagag
      961 tttatgctca agtctataat acaatctgtt aatgagatgc gtaatatgca tgatagaaaa
     1021 tctttagaaa gtatattaaa agaagaattg tttgaaaatg acataactct atcagcaaca
     1081 gaagaacata tattaaaata tatttgcaat aaagataact attctatgac attagaaaat
     1141 tatataaaaa agaataaaag tagatactta aaggctggaa taaaggaaat tgaattagtt
     1201 gaccagttga tggaagtgtt taataatctt gaagagatag agattttgtc taaagaaaaa
     1261 aatatatata aagttaatga taagtactta aaaatgcttg atatagacta atttagataa
     1321 tctggagagg gaatatatga ataataagaa aaaggcattt ttgatttctg gggctataaa
     1381 cgtaatttca ttaattattg ttttttcatt gtttcattat aatattatag aaaatagcac
     1441 acaaaatatg ataataactg taatcttaat ttttattttt gaaatgataa aaataaaaat
     1501 catagagaaa tactataaac tataaacaag aaatatcttt tctaagagca tattgtacaa
     1561 tatgctctta atttagttat atagatttaa tataaataat gtattttttg tagatgattt
     1621 atgtaagatt tatgaccaat ttgtgtatga tttgtgttct ttaatcatga caatattata
     1681 ttgtaaaaaa taaatttaat aaaaaatgac ttggaattta tattctaggt cttttttata
     1741 taaaaattta ataagtttca aatgttggta actttgacag tagaaaacgt ctattgagat
     1801 aacgaggatt ctaattgagg gggagatata ttagcactta aagtagtata tgcaacagat
     1861 ataatcttta taaaagtaat tcgaattaaa atgtaaatat aaagaataat taaaaaaata
     1921 tcgcaattat ttgtt
//
LOCUS       DAEPXF010000336         1914 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain HB27
            SAMN13376389-rid12479423.denovo.344, whole genome shotgun sequence.
ACCESSION   DAEPXF010000336 DAEPXF010000000
VERSION     DAEPXF010000336.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13376389
            Sequence Read Archive: SRR10518432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1914)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1914)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001069624.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001069624.1
            Genome Coverage       :: 315x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/15/2021 01:54:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,165
            CDSs (total)                      :: 4,076
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 89
            rRNAs                             :: 10, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 10 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 19 of 69
            Pseudo Genes (incomplete)         :: 44 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 10 of 69
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1914
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13376389-rid12479423.denovo.344"
                     /strain="HB27"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="China: Hebei"
                     /collection_date="2017"
     gene            28..885
                     /locus_tag="KRL67_003881"
     CDS             28..885
                     /locus_tag="KRL67_003881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBH1524688.1"
                     /translation="MSKDVKVKRNKKYDTFSITMELERPLLYDEVESKDDKNNNRKFN
                     IKNKIFKGIGILFVLILLGFFIWINKTYEPQKLAKEALVSNSKVEVTVNENISFTPKG
                     RKVSKGLILYPGAKVEIESYAPLARKIAESGYEVVIVKMPLNLAILGTNKAQRVMDSY
                     NNIEHWVIGGHSLGGVAASNFARDNKLIEGVVFLASYPMGDELKELGKKVISIWGSKD
                     GVVNFKSLVESKQKLPDDTTYVEIEGGNHAQFGDYGKQKGDNDAIISQEKQLNITANS
                     IIKFLKNIS"
     gene            982..1851
                     /locus_tag="KRL67_003882"
     CDS             982..1851
                     /locus_tag="KRL67_003882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HBH1524689.1"
                     /translation="MEYDMPLYRPPSEAYSLIIQVTLGCSHNKCTFCSMYKSKKFKIK
                     PLEVIKNEIDIFRRYYKHVERIFLADGDALIIPMEKLRDIILYIKEVFPECERITMYG
                     SPKSIEKKSDDELRELRKLGVKMIYLGLESGSNEVLEDIKKGFSSEQLIKVGRKVKKS
                     GIKLSATVIAGLGGTKKTHQHAVDTGKMLGAISPDYVGVLSLMVEPNTELYDLLKAGE
                     FTILEDKAVLQEIKEMIKNIDANEKVVFRSNHASNYVNLKGVLPDDKQRLIDEIDYYL
                     SNQKLKREEYRRL"
BASE COUNT          793 a          185 c          354 g          582 t
ORIGIN      
        1 tattcaaata ttatgggggt tagatttatg agtaaagatg taaaggtaaa gcgtaataag
       61 aaatatgata catttagtat aactatggaa cttgagagac ctttacttta tgatgaggtt
      121 gagagcaaag atgataaaaa taataataga aaatttaaca taaagaataa gatatttaag
      181 ggaataggta tactatttgt attgattttg cttggttttt tcatatggat aaataaaaca
      241 tatgaacctc aaaagctagc aaaagaagct ctagtcagca atagtaaagt tgaagtaact
      301 gtaaatgaaa atatttcatt tacaccaaaa ggaagaaaag tttccaaagg tttaatttta
      361 tatccaggag ctaaagtaga aatagaatct tatgctccac ttgcaagaaa aatagcagaa
      421 agtggatatg aggttgtaat cgtaaagatg ccacttaatc tagctatact tggaactaat
      481 aaggcacaga gagttatgga ttcttacaac aacatagaac attgggtaat aggaggtcat
      541 tcattaggag gagttgcagc atcaaatttt gcgcgtgata ataaattaat tgaaggtgtt
      601 gtatttttag cttcttatcc aatgggtgat gagttgaaag aattaggaaa aaaagttata
      661 tctatatggg ggagcaaaga tggagtagtt aattttaaaa gtcttgtaga atcaaaacaa
      721 aaacttcctg atgatacaac ttatgttgag attgaaggag gaaatcatgc acaatttgga
      781 gactatggaa aacaaaaagg agataatgat gctattataa gtcaagaaaa acaacttaat
      841 attactgcca atagtatcat taaattttta aagaatatat cttaaactaa aattttaaaa
      901 atataatttt ataagttatt atttaaagta aatattaata atataataga atgaatattt
      961 tattacaagg gggattttaa tatggaatac gatatgccac tttacagacc accaagtgag
     1021 gcttatagtc ttataataca agtcacacta ggttgttctc acaacaaatg tacgttttgt
     1081 agtatgtaca aatctaaaaa atttaaaata aagccactag aagtaataaa aaatgaaatt
     1141 gatatattta gaagatatta taaacacgta gaaagaatat tcttagcaga tggagatgca
     1201 ttgataatac caatggagaa attaagagat attattttat atataaaaga agtttttcca
     1261 gagtgtgaaa gaattactat gtatggatct cctaaatcta tagagaaaaa atctgatgat
     1321 gaacttagag aattaagaaa acttggtgtt aaaatgattt atctagggct tgaaagtgga
     1381 agcaatgaag tattagaaga tattaagaaa ggttttagta gtgaacaatt aataaaagtt
     1441 ggtagaaaag ttaaaaaaag tgggattaaa ttatcagcca ctgtaatagc aggtcttgga
     1501 ggaactaaaa aaacacatca gcatgcagtt gatacaggaa aaatgttagg agcaatttca
     1561 ccagattatg tgggtgtact tagtttaatg gtagaaccta atactgaact ttatgattta
     1621 cttaaggctg gagagtttac aatactagag gataaggctg tgttacaaga aattaaagag
     1681 atgataaaga atatagatgc taatgaaaaa gttgtattta gaagtaacca tgcatcaaat
     1741 tatgttaatt taaaaggtgt attacctgat gataaacaac gtctaattga tgaaattgat
     1801 tactatctaa gtaaccaaaa gctaaaaaga gaagaatata gaagactata gtttttgata
     1861 tatcaatcta tattgatagg taaatactga gattgtttag atataaaaaa taaa
//
LOCUS       DAEPXF010000337         1910 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain HB27
            SAMN13376389-rid12479423.denovo.345, whole genome shotgun sequence.
ACCESSION   DAEPXF010000337 DAEPXF010000000
VERSION     DAEPXF010000337.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13376389
            Sequence Read Archive: SRR10518432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1910)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1910)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001069624.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001069624.1
            Genome Coverage       :: 315x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/15/2021 01:54:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,165
            CDSs (total)                      :: 4,076
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 89
            rRNAs                             :: 10, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 10 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 19 of 69
            Pseudo Genes (incomplete)         :: 44 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 10 of 69
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1910
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13376389-rid12479423.denovo.345"
                     /strain="HB27"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="China: Hebei"
                     /collection_date="2017"
     gene            complement(99..1391)
                     /gene="brnQ"
                     /locus_tag="KRL67_003883"
     CDS             complement(99..1391)
                     /gene="brnQ"
                     /locus_tag="KRL67_003883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HBH1524690.1"
                     /translation="MGEKQINFKDVIVIGFALFAMFFGAGNLIFPPYLGVLSGSSWLI
                     AFIGFLFADGGLALLAVIAATKFNGDTSKMFSRAGKGLSIALGCAMVICIGPLLAIPR
                     TAATTYEMGILPTIGSGISPVIFSIVFFALVLVLTIRPSKVVDIVGAILTPALLIALA
                     VLIVKGIVSPLGEIRDSSLIQNVFAEGITQGYQTMDALAASVFASIIIMSVIAKGYTG
                     DKEKMKATISAGVIAVIGMALVYGGLCYLGATVSQQYGQDVQQTALIVAITAALLGTT
                     GKILLAIIVALACLTTAIGLSSAAGQYFSTLTDGKLKYEHIVIVVCVFSAIISNFGVS
                     TIIQFSSPILSMVYPATITLVILALFSNKIKNDNVFKCAAYMALLISVLTVATSFGVK
                     IPLVNNLPFASLGFNWIVPVVIAGIIGNFIPSKSSKTA"
BASE COUNT          638 a          320 c          228 g          724 t
ORIGIN      
        1 tcttttatct acaataattt aataattctt ttaaatataa aaaggaattg cctatataat
       61 ttaaaggcaa ttccataatt atcaattagt ttttattatt atgcagtttt actgctttta
      121 gatggaataa agtttcctat tattcctgct attacaactg gaactatcca gttaaatcct
      181 aatgaagcaa atggtaagtt attaactaga ggaattttta caccaaaact tgttgcaact
      241 gttaatacac ttattaataa tgccatgtat gcagcgcatt tgaacacgtt gtcattttta
      301 attttattac taaataatgc tagtataact agagttatag ttgctggata taccatactt
      361 agtataggag aagagaattg tattattgta cttactccaa agtttgatat gattgcacta
      421 aatacacaaa ctactataac tatatgctca tactttaatt tcccatctgt aagtgttgag
      481 aaatattgac cagctgctga agaaagacct atagctgttg ttaaacatgc taatgcaact
      541 ataatagcca ataaaatttt acctgtagtt ccaagtaaag ctgctgttat tgcaactatt
      601 aaagctgttt gttgaacatc ttgtccatat tgttgagaca cagttgctcc aagataacat
      661 aatccaccat aaactaatgc cattcctatt actgcaatta cacctgcact tattgtggct
      721 ttcatctttt ctttatcacc tgtataacct ttagctatta ctgacataat tataattgat
      781 gcaaatacac tagctgccaa tgcatccatt gtttgataac cttgagttat accttctgca
      841 aatacatttt gtattaaaga actgtctctt atttctccta atggagatac gataccttta
      901 actattaata ctgctaaagc tataagtaaa gctggagtta atattgcccc aactatatct
      961 acaacttttg atggtcttat tgttaaaacc aatactagag caaagaatac tatagagaat
     1021 attactgggc ttattccaga acctattgta ggtaatatac ccatttcata agttgttgct
     1081 gctgttctag gtattgctaa aagtggccct atacagatta ccattgcaca tcctaatgca
     1141 atacttaatc ctttgcctgc tcttgagaac atcttcgatg tgtctccatt gaacttagtt
     1201 gctgctataa ctgctaataa tgccaaacca ccatctgcaa aaaggaatcc tatgaaagct
     1261 attagccagc tacttccaga taaaactcct agatatggag ggaagattaa gttccctgca
     1321 ccaaaaaaca ttgcaaatag tgcaaatcca attacaatta catctttaaa attgatttgt
     1381 ttctcaccca tttaaagcac ccccatttga attttactac cttttactaa aaaataagtc
     1441 caatctcttc attaaatgaa aaccttttga cgagatttta tttaattata catttttgac
     1501 ttaaatttgt caattatgtt tcataattta taaaatcttc acaatattta atacaacttt
     1561 cttaattaaa caaaatcaac ctgtatactt taaacatagt tggttttttg ttttttctct
     1621 aatttttctt gataaaatta attattctga ataattaaaa cattaagatt ttttagatta
     1681 ttcgttttat tattaagtgt attgctttat atactgtata tatcactctc cttttttaca
     1741 ttcatataat ctttttaatt caatttaaat tttaaatatt ttgtgtttat tatataatct
     1801 gaaaaaaaag cagtcaatag catttttaat ttttttaatt ttcaaaatta tgattcttaa
     1861 aaaatcgttt tccttttgtc atagcaccta tttacgttaa ttcttgtagt
//
LOCUS       DAEPXF010000338         1884 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain HB27
            SAMN13376389-rid12479423.denovo.346, whole genome shotgun sequence.
ACCESSION   DAEPXF010000338 DAEPXF010000000
VERSION     DAEPXF010000338.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13376389
            Sequence Read Archive: SRR10518432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1884)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1884)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001069624.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001069624.1
            Genome Coverage       :: 315x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/15/2021 01:54:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,165
            CDSs (total)                      :: 4,076
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 89
            rRNAs                             :: 10, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 10 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 19 of 69
            Pseudo Genes (incomplete)         :: 44 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 10 of 69
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1884
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13376389-rid12479423.denovo.346"
                     /strain="HB27"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="China: Hebei"
                     /collection_date="2017"
     gene            228..371
                     /locus_tag="KRL67_003884"
                     /pseudo
     CDS             228..371
                     /locus_tag="KRL67_003884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891255.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
     gene            1019..>1884
                     /locus_tag="KRL67_003885"
     CDS             1019..>1884
                     /locus_tag="KRL67_003885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBH1524691.1"
                     /translation="MIVVEKAYKFRMYPNKKQQELINKTFGCCRFVYNKYLAKRIDVY
                     KNDKETFTYKQCSSDLTNLKKELKWLKEPDKFSLQNALKDLDNAYEKFFKEKAGFPKF
                     KSKKINRFSYKTNFTNGNIMYCGQHIKLPKLGMVKIRDKQVPQGRILNATISKEPSGR
                     YYVSLCCTDVDIEAFENTNNHIGLDLGIKEFCISSCGDFIENPKYLKKSLSKLAKLQR
                     ELSRKTIGSLNRNKARLKVARLQEHIANQRNDFLQKLSTKLIKENDIICIEDLQVKNM
                     IRNRKLSRLISDV"
BASE COUNT          803 a          220 c          292 g          569 t
ORIGIN      
        1 tacaagtata gtctaaatag agctgttttt aagcaaagtt ttataaatga ctattaaagt
       61 ataaagtaac agtataaagt ttacttataa attaaatgta aacctaaata cttataatag
      121 ttgacaaaaa aacgtcttta ttttatacta aaacaaagct ttaatagaag ctttgtatat
      181 gtacatataa actaaatgaa ataagacata tgaagggaat tataaaaatg aaaaatacta
      241 aagtaaatat tcaagattta actaaaatgt caatatgtgt agcattattg tgtatatcat
      301 cttacatata tataccattg ccatttactc cagcaggagt aacagcacaa acaataatga
      361 taaatttgat tagaaaatag ctacgcacac ttgtgacttt agtcatgagt tagtagctaa
      421 aatagtcagc atatagggaa acctatatgt agagatagga tagaacctat ccaatactac
      481 ttgaattgct gggaacccct aaagctaatc aaactaaaac ataaggatga aataaaccta
      541 agtgtgaaag ttacgaaagt agaaaaaatt ggttagatgg tataaggtta aaccctaagt
      601 actttaaaat gggcaatcag caggtaaact ccgaatagga gaaacttcaa cgactattcc
      661 tcttgaggga agtacactac aagctattgg tagtggaagt gggtagacct taacggataa
      721 tgccgaagga taagatatag tctgtgctta catgaaagtg taagaagttc ataagagaac
      781 tgcataggtg gtagcgaact tatgtgaacg acaacctcaa aaatgataaa agttaagact
      841 tacggttctt aaatatgtta tttaaaatta aatataaaac cttctaaata attgttttaa
      901 ataattacat ttgttaacat tattataggt gattaataaa ttgttcacca cagcattata
      961 taatatatta aatgtgataa aatcattaaa aagtgtatca aaaaacaatt aaagtgaggt
     1021 gatagtagtg gaaaaagctt ataagtttag aatgtatcca aataaaaaac aacaagagtt
     1081 aattaacaaa acttttggat gttgtagatt tgtatataat aaatatcttg ctaaaagaat
     1141 agatgtttat aaaaacgata aagaaacttt tacttataaa cagtgtagtt ctgatttgac
     1201 caacttaaaa aaagaattaa agtggcttaa agaacctgac aaattttcac tacaaaacgc
     1261 cttaaaagat ttagataatg catatgaaaa attctttaaa gaaaaggcag gatttcctaa
     1321 atttaaatca aagaaaatta atagattttc atataaaact aactttacaa atggaaacat
     1381 tatgtattgt ggtcaacata taaaactacc taaacttggt atggttaaga taagggataa
     1441 acaagtacct caaggtagaa tacttaatgc tactatatct aaagaaccaa gtggtagata
     1501 ttatgtatca ttatgctgta ctgatgtaga tattgaagca tttgaaaata ctaataatca
     1561 tataggttta gatttaggaa ttaaagaatt ttgtatctca agttgtggag actttataga
     1621 aaatcctaag tatcttaaga agtcattaag caaacttgct aaattacaaa gagaattatc
     1681 gagaaaaaca atcggtagtt taaatagaaa taaagcaaga ttaaaagttg caagacttca
     1741 agaacatata gctaatcaaa gaaatgattt tctacaaaaa ctatctacta aattaataaa
     1801 agagaacgat attatttgca tagaagattt acaagtaaag aatatgatta gaaatcgtaa
     1861 actatctcgc ttaatttctg atgt
//
LOCUS       DAEPXF010000339         1856 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain HB27
            SAMN13376389-rid12479423.denovo.347, whole genome shotgun sequence.
ACCESSION   DAEPXF010000339 DAEPXF010000000
VERSION     DAEPXF010000339.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13376389
            Sequence Read Archive: SRR10518432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1856)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1856)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001069624.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001069624.1
            Genome Coverage       :: 315x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/15/2021 01:54:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,165
            CDSs (total)                      :: 4,076
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 89
            rRNAs                             :: 10, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 10 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 19 of 69
            Pseudo Genes (incomplete)         :: 44 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 10 of 69
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1856
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13376389-rid12479423.denovo.347"
                     /strain="HB27"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="China: Hebei"
                     /collection_date="2017"
     gene            complement(553..897)
                     /locus_tag="KRL67_003886"
     CDS             complement(553..897)
                     /locus_tag="KRL67_003886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH1524692.1"
                     /translation="MNYRLKKSVTTILSLLIMTILLPTNASASKVEDSSMKVMYSEKQ
                     VTDMDKLYDMAKNGITDVKSSNEKGIIKNARTGESIYVDSISTTQLLEVKQSKNLTSK
                     IYSKTVFLILIQ"
     gene            1024..1407
                     /locus_tag="KRL67_003887"
     CDS             1024..1407
                     /locus_tag="KRL67_003887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH1524693.1"
                     /translation="MRKKFFIGILIVLIISVIAILNIKNDKNVFIGESENWKAKFVTT
                     EGHSKLEIKYKNSKDVVEQKFNYSYQFIDGEDAVVVVKNEILEAGQEKAIKIQRPGLY
                     SNRESSYKLIISWNGNEETINLKKK"
BASE COUNT          700 a          231 c          233 g          692 t
ORIGIN      
        1 taaaataaaa ttcatatatt aagaaatatt aatagtaaat aagcaattgt tactataacg
       61 taggaattat ctttaaaaac acaaattaat ttacattttc taattttaaa gtatatgtat
      121 atctttgatt ttaataaaat tcacttaaaa tattttaagg ttgaacaatg aaattgaatg
      181 cccaagaact tccttctctt actaatgttg caattaattc aaacccacaa ttgtattgaa
      241 ctgaatctct acctactggt ttccaactag aaggtgcatt atatgagaaa gaatttgatg
      301 ttggctcttt cttttgttgc tgcttaattc ctagcccatt agaaataaat ccagtttgat
      361 ttatatgccc ccttctattt gttacacgaa tctggccgtc tgcaatagac cagtttccag
      421 aaactttact aacggaaata tattgaaatg ctccatctcc ttttgatatt gtatatgtaa
      481 ctgtaccaga tgcttttaca ccaccagttt tatcccatcc atcttctgat atacttgctt
      541 ttctaagagg ctttattgta ttagtatcaa aaaaactgtc ttagaatata tctttgatgt
      601 taaattttta gattgtttaa cttctaataa ttgagttgtt gatatagaat caacgtatat
      661 ggactctcct gttcttgcat ttttgattat acctttttca ttggaacttt taacatcagt
      721 aattccattt ttagccatat cgtataattt gtccatgtct gttacttgtt tctctgaata
      781 cattactttc atagatgaat cttctacctt actagcactt gcatttgttg gtaatagtat
      841 tgtcattatg agtaaagata aaatagttgt tacagatttt tttaacctat aattcatatt
      901 aatccttact ttttatttgt tataacagac attctattgt aatagaatat aaatccacaa
      961 tatttttaaa attagtattg aataactatt ctcatgttat aattattcaa aggagggatt
     1021 gatatgagga aaaaattctt tataggtatt cttatagttt taataatctc tgtgatagct
     1081 atacttaata taaaaaatga taaaaatgtc tttattggag aatcagaaaa ttggaaagct
     1141 aaatttgtaa ctactgaagg acactcaaaa ttagaaataa aatataaaaa tagtaaagat
     1201 gtagttgaac aaaaatttaa ttattcctat caatttatag atggtgaaga tgcagttgta
     1261 gttgtaaaaa atgaaatttt agaagcaggt caagaaaaag caataaaaat acaaagacca
     1321 ggtctttatt caaatagaga atctagttac aagctaatta tatcttggaa tggaaatgag
     1381 gaaactataa acttgaaaaa gaaataacca tattatcaat ttttaaaaaa ttattattca
     1441 aattcttata aaaagagtgt ttagaaaaat gaaacactct tttttgtata agtttttgaa
     1501 aactataaat gaaatatatc aaaatcgttc gaaaaattac tgaaataaac tcttaaatat
     1561 attataaatt aaggtatatt atacatgaat ttattgggag tttatgagtt ttaaaattgg
     1621 aaaccaaaca ataagggcga aaaatacttt aaggaaaata tataaagttc tataaattat
     1681 caaaaatatt tcgcaaaaat tgataattat tacaaataaa ttgataatat taataatatt
     1741 agaaatatcg aaattgaaca aactaggatt aaagctcaat tagaaaaatc cactaatcct
     1801 gagaatgctt tgttattctc atttattact gttgtttttt cattatttgc taattt
//
LOCUS       DAEPXF010000340         1855 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain HB27
            SAMN13376389-rid12479423.denovo.348, whole genome shotgun sequence.
ACCESSION   DAEPXF010000340 DAEPXF010000000
VERSION     DAEPXF010000340.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13376389
            Sequence Read Archive: SRR10518432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1855)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1855)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001069624.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001069624.1
            Genome Coverage       :: 315x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/15/2021 01:54:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,165
            CDSs (total)                      :: 4,076
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 89
            rRNAs                             :: 10, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 10 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 19 of 69
            Pseudo Genes (incomplete)         :: 44 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 10 of 69
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1855
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13376389-rid12479423.denovo.348"
                     /strain="HB27"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="China: Hebei"
                     /collection_date="2017"
     gene            205..819
                     /locus_tag="KRL67_003888"
     CDS             205..819
                     /locus_tag="KRL67_003888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HBH1524694.1"
                     /translation="MEEVIAKIITIENGFKEIENIAKKIVKNNDSKKCYYLSIYLYRS
                     EYYQVRELAVFILGFISVTISEALLFLKDNVSKDENWRVQEILAKSFDYYSSEVGYEK
                     ALPVIKEWLNSDSPNIKRAVTEGLRIWTSRDYFKSNPDIAISLLSSLKEDDSEYLRKS
                     VGNALRDISKKYPDLVKKELDSWDISNKKVEFVYKLASKCILAK"
     gene            837..1199
                     /locus_tag="KRL67_003889"
     CDS             837..1199
                     /locus_tag="KRL67_003889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBH1524695.1"
                     /translation="MKLRDEYTCPLELTHDMIKGKWKSIILWRLRIGPTSLSKLEKEI
                     NGINQKMLLEQLKELIEFGLVDKKTYEGYPLKVEYSLTPIRGKQILDALAIMQDIGIN
                     FMLDNGMEDILKEKGVLK"
     gene            1305..1802
                     /locus_tag="KRL67_003890"
     CDS             1305..1802
                     /locus_tag="KRL67_003890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhB/YbcL family Raf kinase inhibitor-like
                     protein"
                     /protein_id="HBH1524696.1"
                     /translation="MKVTSTGIVNGVIEDKYGKRGTQFNEGGMPTYSLPLKFEDAPKN
                     TVSFAVFLEDKDSVPVCGFAWIHWLAVNITKDELKENESVTATDFVQGTTSWHGKLGG
                     LDRLEASVYGGMAPPDRPHIYEIHVFALDTILDLEKGFYMNEFFKAIEGHVLDTFTLK
                     GSYSN"
BASE COUNT          716 a          207 c          323 g          609 t
ORIGIN      
        1 aaatacaact ttataatata aaacttataa taaaagtaaa tttatatgtt aaaggaggtt
       61 gattttttat tattttttat ttatgctagt actaaagact tgcaaacgtt ttttgacaag
      121 tctttatttt gtctaaatta aacaaacttt tttacaaata aatacataaa atttaaacta
      181 atctacttat tggaggagat aaaattggaa gaagtaattg caaaaattat tactatagaa
      241 aatggattca aagaaattga aaacattgct aaaaaaattg taaaaaataa tgactcaaaa
      301 aagtgttatt atctatcaat ctacttatat aggtcagaat attatcaagt aagagaattg
      361 gctgtgttta tacttggttt tatatcagta actatttctg aagctttgtt atttttaaaa
      421 gacaatgtga gtaaagatga aaactggaga gtacaagaaa tattagctaa atcatttgat
      481 tattattcca gtgaagtagg atatgaaaag gcacttcctg ttataaaaga atggcttaat
      541 tctgatagtc caaatataaa aagagctgtg acagaaggtc ttagaatttg gactagtaga
      601 gactatttta aatctaatcc tgacattgct atatcactac tttctagttt aaaagaagat
      661 gatagcgaat acttgagaaa gtctgtaggt aatgctctta gagatataag taaaaaatat
      721 ccagatttag taaaaaaaga attagattca tgggatatat caaataaaaa agttgagttt
      781 gtatataagc ttgcttcaaa atgtatcttg gcaaagtaaa atggaggtga acttatttga
      841 aacttcgtga tgagtatact tgcccattag aactaacaca tgatatgata aaaggcaaat
      901 ggaaatctat tatattatgg agattacgca taggaccaac ttccttatca aaacttgaaa
      961 aggaaatcaa tggtattaat caaaagatgt tacttgaaca acttaaagaa ttaattgaat
     1021 ttggtcttgt agataaaaag acatatgaag gttatccact taaagttgaa tactcactta
     1081 cacctattcg agggaaacaa atattggatg ctttggctat aatgcaagat ataggaatta
     1141 actttatgtt ggataatggt atggaagata ttcttaagga aaaaggtgtt ttaaagtaaa
     1201 tagataccca caaaaaagta agtaatttac cattattttc aactggtata taattaaatt
     1261 atagagatat tctattaatt tattataata ggaggtaaat tattatgaaa gtaactagta
     1321 caggtatagt aaatggtgtt atagaagata aatacggtaa aagaggaaca cagttcaatg
     1381 aaggtggtat gcctacatat tcactacctc taaagtttga agatgctcca aagaatacag
     1441 tatcctttgc tgtgttttta gaagataaag actctgttcc tgtatgtgga tttgcatgga
     1501 tacattggtt ggctgtaaat ataacaaaag atgagttaaa ggaaaatgaa agtgtaacag
     1561 ctactgattt tgtacagggg actactagtt ggcatggaaa actaggaggt cttgacagat
     1621 tagaggccag tgtatatggc ggaatggctc ctccagacag acctcatatc tatgaaatac
     1681 atgtatttgc acttgatact atccttgacc ttgaaaaagg attctatatg aatgaatttt
     1741 tcaaagctat tgaagggcat gttttagata catttacact aaaaggaagt tattctaatt
     1801 aattaggttg actatagcta atcattaatt aagtgcctac atttaattga tgatt
//
LOCUS       DAEPXF010000341         1822 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain HB27
            SAMN13376389-rid12479423.denovo.349, whole genome shotgun sequence.
ACCESSION   DAEPXF010000341 DAEPXF010000000
VERSION     DAEPXF010000341.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13376389
            Sequence Read Archive: SRR10518432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1822)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1822)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001069624.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001069624.1
            Genome Coverage       :: 315x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/15/2021 01:54:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,165
            CDSs (total)                      :: 4,076
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 89
            rRNAs                             :: 10, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 10 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 19 of 69
            Pseudo Genes (incomplete)         :: 44 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 10 of 69
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1822
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13376389-rid12479423.denovo.349"
                     /strain="HB27"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="China: Hebei"
                     /collection_date="2017"
     gene            complement(285..1613)
                     /locus_tag="KRL67_003891"
     CDS             complement(285..1613)
                     /locus_tag="KRL67_003891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBH1524697.1"
                     /translation="MGSNELKKTLGVSAALSTVVGSVIGAGVFFKPQAVYTLTGGAPG
                     LGILAWLIAGIITITAGLTAAEVSVAIPKTGGMMVYIKEIYGEKLGFLTGWMQIVLFY
                     PGMMAALGVIFGEQASALIGSPSLLLPIAIGIIVIVAGLNMLGSKTGGVIQTVSTICK
                     LIPLILIMVVGFIKGGGNNPILTPMVGEGLSLGSVLGQVLIAILFAFDGWMNVGTLAG
                     EMKNPGKDLPKAIIGGLSVVMAVYFIINLAYLWVLPANELANYASPASAVAEVIFGSM
                     GGKIISVGILISVFGALNGFLLTGSRVAYTLATDKTLPKYGIFSKLNSAQVPANAIAL
                     VSVIASIYALSGQFNLLTDLAVFATWIFYVLTFIGVMKLRKTHPNIPREYKVPLYPIV
                     PIIAIASGIFVVVNQLCFAGMKTTMISIGGLVITAIGLPVYAYMTRGVKR"
BASE COUNT          668 a          326 c          205 g          623 t
ORIGIN      
        1 ataaaagcta tgtataatat ttgttatata gatatcactt agtttgataa gaaaacattt
       61 taaaataaag tattttaaag caaataattt tttagatgtt taattttata atccaaaaac
      121 taaaaataaa gtgaatacct taaaataaac tttttaattc ttgaaacact ctttttttat
      181 aaaatcaaac atatctttac cattttaaca ataaaaaaga gacttatctc tatttgagat
      241 agtctctctt attttaatta taaagcattt gtaaactact tggcttatct ttttactcct
      301 ctagtcatat aagcatacac tggtaagcct atagctgtta ttacaagacc tcctatagat
      361 atcatagtag ttttcatacc tgcaaaacac aattgattaa ctacaacaaa tataccactt
      421 gctatagcta ttataggaac aataggatat aatggaactt tatactctct tggtatattt
      481 ggatgagtct ttctaagttt cataacccct ataaaagtta atacatagaa tatccaagta
      541 gcaaatacag ctaagtcagt taataaatta aattgacctg acaaagcata tatagatgca
      601 ataacagata ctaatgctat tgcatttgct ggaacttgag cagaatttaa tttagaaaat
      661 ataccatact ttggtaaagt cttatctgta gctaaagtat atgctaccct tgaaccagtt
      721 agtaaaaatc cattcaatgc tccaaacact gaaattaata tacctacaga tattattttc
      781 ccacccattg aaccaaatat aacttcagca actgctgatg caggagaagc ataatttgct
      841 agttcattag ctggtaatac ccaaagatat gctaaattta ttataaaata aacagccata
      901 acaactgata atccacctat tatagctttt ggaaggtctt ttccaggatt cttcatttct
      961 ccagctaaag taccaacatt catccatcca tcaaaagcaa ataatatagc aattaaaact
     1021 tgacctagta cactacccag acttaatcct tcaccaacca taggtgtaag tattggatta
     1081 tttccaccac ctttaataaa tccaacaacc attattaaaa taagtggtat taacttacaa
     1141 attgtagaaa cagtttgtat gactcctcca gtttttgaac ctaacatatt aagccctgca
     1201 actattacta taattccaat tgctattgga agtaaaagtg atggactacc aattagtgct
     1261 gatgcttgtt ctccaaatat aacacctaat gctgccatca tccctggata aaataataca
     1321 atttgcatcc atccagttaa aaaacctaat ttttctccat aaatttcttt tatatatacc
     1381 atcattccac cagtttttgg tatcgcaact gatacctctg cagctgtaag accagctgtt
     1441 atagttatga ttccagctat taaccatgcc aatataccaa gacctggagc tcctccagtt
     1501 aatgtataga ctgcttgtgg tttaaaaaat acgcccgctc ctataacgga accaacaaca
     1561 gttgataatg ctgctgatac gccaagagtc tttttcagtt cattacttcc catttttttc
     1621 ctcctcatta atcctattta attttaattt catagttaaa taagcatata ttatgccaat
     1681 atatagaaaa atagttttat tatttattaa aataaaataa actttattta gcttttgtaa
     1741 tcttttactt ttagttatgt ttaaatataa aaaaattata aaaaacaaaa atccaaatat
     1801 aatagtactt ctaattaaat gt
//
LOCUS       DAEPXF010000342         1817 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain HB27
            SAMN13376389-rid12479423.denovo.350, whole genome shotgun sequence.
ACCESSION   DAEPXF010000342 DAEPXF010000000
VERSION     DAEPXF010000342.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13376389
            Sequence Read Archive: SRR10518432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1817)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1817)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001069624.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001069624.1
            Genome Coverage       :: 315x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/15/2021 01:54:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,165
            CDSs (total)                      :: 4,076
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 89
            rRNAs                             :: 10, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 10 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 19 of 69
            Pseudo Genes (incomplete)         :: 44 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 10 of 69
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1817
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13376389-rid12479423.denovo.350"
                     /strain="HB27"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="China: Hebei"
                     /collection_date="2017"
     gene            complement(720..1499)
                     /locus_tag="KRL67_003892"
     CDS             complement(720..1499)
                     /locus_tag="KRL67_003892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HBH1524698.1"
                     /translation="MSYKKIAIITGANSGFGKEFLKLLINEEEITEIWAVARNKARLN
                     QLVDEFGSKVKVFSKDLSKIEEVKEIGTFLSKENVCIKYLINNAGFAKFCSYNDLSID
                     ESINMIDLNISAVVALGLIYIPYMQKGSHIMNISSQASFQPLPYQNIYSSTKAFIRNY
                     TRALNVELKSKGINAIAVCPGWMSTNLFKRGIVVAEKGTKNFPGIVSPDVVAKKALKD
                     AKKNKDISVYGINTKSSHLLAKLLPQKMMMKVWLMQQHIKE"
BASE COUNT          657 a          258 c          218 g          684 t
ORIGIN      
        1 attaaaagaa ggattaagac taataacaat tcaaaataaa taacttatgt aagaaccgta
       61 ggaactacgg ggatagcctg tggagaatta gtaagacata ttttagtatg caaaattcta
      121 tgaagcagga accctgtgac tttagtcatg ggaggttcag gctgatgttt taataataag
      181 tctagtttct gatgatggta tattaaatga tggtacaaaa cttaaagaca caccatttaa
      241 agatgaaatc tttactttag atgagattgt tgaaattaaa aactattata gaattaaaaa
      301 gattatcgtt acacatattg acgaaatgtg gggaaaatca tatggttatt acagtgaatt
      361 agaaaagaaa cttgataata tttattttgc atatgatggt atggaaatta tagtctaaat
      421 cattaagtaa taagcataat gtaaaataaa aaaacttcaa tttaacagta tagtcaaaat
      481 atgtaatatc aaaatatcac agtttttaca aaatatcata gtatttatct ttataaaatt
      541 tcaaactcta catatttcaa tacctcaata aagtaaaatt aaaaatatat tttatataca
      601 ttatagacgg cttatcggca ctagataagc cgttttgtca caattattat ttactcaaca
      661 aaaattctca taatttataa cagattgaat ctctaactga tacttaatta caaaaactct
      721 cattccttaa tatgttgttg cattaaccaa accttcatca tcattttttg tggcaatagt
      781 tttgctaata aatgactgga ttttgtattt ataccataca cagaaatatc cttatttttc
      841 tttgcatcct tcaatgcttt ttttgcaaca acgtctgggg acactatgcc tgggaaattt
      901 tttgtccctt tttcagctac aactatacct ctcttaaata gatttgtact catccaacct
      961 ggacatacag caattgcatt tatcccttta ctcttaagct caacatttaa tgctcttgta
     1021 taatttctta taaacgcttt agttgaacta taaatatttt gatatggcaa tggctggaat
     1081 gatgcttgtg aagaaatatt catgatatgg cttccctttt gcatatatgg aatatatata
     1141 agacccaatg ctacaacagc actaatattt aaatcaatca tattaataga ttcatctata
     1201 cttaaatcat tgtatgaaca gaattttgca aaaccagcat tgtttataag gtattttata
     1261 catacatttt ctttacttaa aaatgtacca atttctttta cttcttctat ttttgataaa
     1321 tcttttgaaa aaacttttac cttactgcca aattcatcta ctaattgatt taatctcgcc
     1381 ttgtttcttg caactgccca tatttctgtt atttcttctt catttattaa taactttaga
     1441 aactctttac caaaaccact attagcaccc gttataattg caatcttctt ataactcata
     1501 attattctcc tttaccataa gtttatacgc tcaaactgta aacttatttt ttacaaataa
     1561 aacaccctct attcagatgt tatattcata tcatttgaga tattatattt atatcatttt
     1621 caatagttgt tatatagcat aaaaaatagt gttaatatta tagaatcgat attgattata
     1681 tttaaaaagt agtgttaatt atattataga atcagtattg attatattgt aaaaagtagt
     1741 attaataata ttgtagaatc agtattgatt atatttaaaa aagtagtgtt aattatatta
     1801 tggaatcagt attgatt
//
LOCUS       DAEPXF010000343         1769 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain HB27
            SAMN13376389-rid12479423.denovo.351, whole genome shotgun sequence.
ACCESSION   DAEPXF010000343 DAEPXF010000000
VERSION     DAEPXF010000343.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13376389
            Sequence Read Archive: SRR10518432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1769)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1769)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001069624.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001069624.1
            Genome Coverage       :: 315x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/15/2021 01:54:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,165
            CDSs (total)                      :: 4,076
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 89
            rRNAs                             :: 10, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 10 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 19 of 69
            Pseudo Genes (incomplete)         :: 44 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 10 of 69
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1769
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13376389-rid12479423.denovo.351"
                     /strain="HB27"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="China: Hebei"
                     /collection_date="2017"
     gene            <1..405
                     /locus_tag="KRL67_003893"
     CDS             <1..405
                     /locus_tag="KRL67_003893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH1524699.1"
                     /translation="WISEYDYNDIDTLTKINNLEIIFTVIEKVVRGLFVEMLCKLQNI
                     CKINDSTKLHNKLQYIKDILYNGLKENIFLEKLNYKLLDRRLTKKINLYMKKNAELYE
                     SRVETILLNIREYVDLDEFELFIIDKLIQKIK"
     gene            589..1264
                     /locus_tag="KRL67_003894"
                     /pseudo
     CDS             589..1264
                     /locus_tag="KRL67_003894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438019.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
BASE COUNT          809 a          159 c          220 g          581 t
ORIGIN      
        1 tggataagtg aatatgatta taatgatatt gatacattaa ctaaaattaa taaccttgaa
       61 ataatattta ctgttataga aaaggtagta agaggtttgt ttgttgaaat gctatgcaaa
      121 ttacagaata tatgtaagat caatgacagt acaaaattac ataataaatt acaatatatt
      181 aaagatatat tatataatgg acttaaagaa aatatatttt tagaaaaact aaattataaa
      241 ctactagata gaaggttaac gaaaaaaatt aatttatata tgaaaaaaaa tgctgaatta
      301 tatgaaagta gagtggaaac tatactacta aatattagag aatatgtaga tttagatgag
      361 tttgaattat ttataataga taagctaata caaaaaatta aataataaat ccaatcgtag
      421 gtgttatccc tatgaatcgt atccaccatt gaaatccacg aatacctatg tccaattgga
      481 tataggtatt tttatacatt tttaatttaa atattattga taaatacatt taaaaagtgt
      541 taacctaaag tgaagaatct aaataaaaat acaatattag gagaggttat cattacaata
      601 gcagcttgtg atgatgaaaa agaaatacaa atcgtaataa aaaatcaagt tgataatata
      661 ttaaaaaata aaaacaaaga atatcgaatt aaaaaaatat agctcaaaag aagaaccatt
      721 acaaagttaa ttgagaggat acaatactct tagatataaa aatggcagaa ctaaatagta
      781 tagatatcgc aagaaacata agaaaagtag atgataatat ttataacatc tctaataaat
      841 tatgtacagg aaagatatga agtaagagct tattgatact tattaaagcc aataaatttt
      901 caagaactag aaaaacacat attaacatgt atcaaaaaaa ttgaaaaaaa taaaaataag
      961 tctaacacat acaaaatcta ctctagcaaa aaaatatata gaagttcaaa aaaagaacat
     1021 gctaagacat acaaaaaata aaacttttga tgctatatgt atcctagaaa aaatagaaaa
     1081 agatttaaat atagaggaac ttgtaagatg tcataaaagt tatataatta acttaaacta
     1141 tgtagaaaat ataaagtcaa atactgcaat attagacagt caagaagaag tacctattag
     1201 cagatataga tataaagata taaaagagag atttctaaaa tttattggtg atagaatatg
     1261 ctaaaccata taaataaaat attattaata ttatcattgt ttacaccaat aatgatacta
     1321 aaaagtatat taacttacga taaagataca gataagacta agaaaatatc tttttttact
     1381 gtattattag cttatcttat aactaaaatc attatattat ttaacgttga taagacccat
     1441 gattataata tgaagaaaaa tctatatgat ataatctatt atttgatatt tattatttgg
     1501 gaacttacta taattatttt tataagatag gaattgttaa atatatagtt tcatttttct
     1561 tatttaacgt atatcaccct ttaataaaag atgttatatc tataaattta ttttttgtag
     1621 ttacaagtaa tgtaatatca tcagaggaag ctctattagt gtctagttat gataatgcta
     1681 aagtagatat atactattat cttataataa tagttatata tagtagcttg tgctatattt
     1741 aaatttatta ataaaagatt gggattaga
//
LOCUS       DAEPXF010000344         1734 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain HB27
            SAMN13376389-rid12479423.denovo.352, whole genome shotgun sequence.
ACCESSION   DAEPXF010000344 DAEPXF010000000
VERSION     DAEPXF010000344.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13376389
            Sequence Read Archive: SRR10518432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1734)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1734)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001069624.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001069624.1
            Genome Coverage       :: 315x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/15/2021 01:54:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,165
            CDSs (total)                      :: 4,076
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 89
            rRNAs                             :: 10, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 10 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 19 of 69
            Pseudo Genes (incomplete)         :: 44 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 10 of 69
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1734
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13376389-rid12479423.denovo.352"
                     /strain="HB27"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="China: Hebei"
                     /collection_date="2017"
     gene            101..1606
                     /gene="ade"
                     /locus_tag="KRL67_003895"
     CDS             101..1606
                     /gene="ade"
                     /locus_tag="KRL67_003895"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HBH1524700.1"
                     /translation="MSPGFIDAHMHIESTMVMPIELSKKLLKSGTTTIIADPHELVNV
                     KGASAIDFLLESTKDIPLNVYIMMPSSVPATSFETNGVGKFSAKDMESYVNNPRILGL
                     GEVMCFNDVINSENEILDKLELFKNKVVDGHAPNINGKSLQTYVCAGIENDHECITFD
                     EVYEKLRAGLKILIREGSAAKNLKSIVSGMLKHNLPIEEFMFCTDDKHLDDIEKQGHI
                     RWNIKCAIDLGMEPVRAIKVATYNSARAYGLRKIGAIGAGYKADIVVLNDLDKMEVDS
                     VYKDGNLVNEEMFSNYNYEIKDKELLNTVKFKYINKEKIQLKVSEKNYVMEIVSYQIL
                     TNKVYESLPCADGYFVPNKEYSKLCVVERHRMTGNVSIAPLKGFGIKNGAIATTVAHD
                     SHNIIVAGDNDDDILVAINYLKEIQGGYVIVSNGKVLAHLSLQVAGLISTFTAEEVQE
                     ITGNMLEIARKMGVPEYVDPFITLSFMALPVVPQIRLTDLGLFDVEEFKFI"
BASE COUNT          661 a          190 c          336 g          547 t
ORIGIN      
        1 gcctgtggag aattagtaag acatatttta gtatgcaaaa ttctgtgaag caggaaccct
       61 gtgactttag tcatgggagg ttcagtgtag caacaaatat atatcacctg gttttattga
      121 tgcacatatg catattgaat ctacaatggt aatgccaatt gaattatcaa aaaaattatt
      181 aaaatcaggt acgacaacta tcatagccga cccacatgag ttggtcaatg taaaaggagc
      241 tagtgcaata gattttttat tagaatcgac aaaagatata ccacttaatg tatatattat
      301 gatgccttca tccgtacctg caacaagctt tgaaactaat ggagttggta aatttagtgc
      361 aaaagacatg gagagttatg taaataatcc tagaatttta ggtttaggag aggtaatgtg
      421 cttcaatgat gttataaatt ctgaaaatga aatcttggat aaactagagc tttttaaaaa
      481 taaagttgta gacggacatg cacctaatat aaatggtaaa tcacttcaga catatgtatg
      541 tgcaggaata gaaaatgacc atgaatgtat aacttttgat gaagtatatg aaaaattgag
      601 agcaggatta aaaattttaa taagagaagg aagtgcagca aaaaatttaa agtctatagt
      661 aagtggtatg ctaaagcata acttgccaat tgaagaattt atgttttgta cagatgataa
      721 gcacttagat gatatagaaa aacaaggtca cataagatgg aatataaaat gtgcgattga
      781 tttaggtatg gaacctgtta gggctataaa agttgcaact tataattctg caagagcata
      841 tgggttaagg aaaattggag caataggtgc gggttataag gcagatattg ttgtattgaa
      901 tgatttagac aaaatggaag tggatagtgt atataaagat ggtaatttgg taaacgaaga
      961 gatgttttct aattacaatt atgagattaa agataaagaa ttgcttaata cagttaagtt
     1021 taagtatatt aataaagaaa aaatacagtt aaaagtaagt gaaaaaaatt atgtaatgga
     1081 gattgtatct tatcaaattt tgacaaataa ggtatatgaa tctttaccat gtgcagatgg
     1141 atattttgtt ccaaataaag agtattcaaa actttgtgtt gttgagagac atagaatgac
     1201 tggaaatgta tcaattgctc cactaaaagg atttggcata aaaaatggag caattgctac
     1261 tactgtggct catgattctc ataatattat agttgcaggg gataatgatg atgacattct
     1321 agtagccata aattacttaa aagaaataca aggtggttat gtaattgtat caaatggaaa
     1381 agttcttgca cacctaagtc ttcaagtagc tggattaatt agtactttta cagctgagga
     1441 agtacaagaa attactggta atatgttaga gatagcaaga aaaatgggag ttccagagta
     1501 tgtagaccct tttattacac tttcttttat ggcacttcca gttgttccac aaataagact
     1561 tacagattta ggtctatttg atgttgagga atttaaattt atatagataa tataaaagta
     1621 ataataaatg aggctttctc aaaatagaga taagtctctt tttattgctg aaatgatgaa
     1681 aatatatttg atttcataca aaaaagagca tcttatgaac taaaaatttt attt
//
LOCUS       DAEPXF010000345         1733 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain HB27
            SAMN13376389-rid12479423.denovo.353, whole genome shotgun sequence.
ACCESSION   DAEPXF010000345 DAEPXF010000000
VERSION     DAEPXF010000345.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13376389
            Sequence Read Archive: SRR10518432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1733)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1733)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001069624.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001069624.1
            Genome Coverage       :: 315x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/15/2021 01:54:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,165
            CDSs (total)                      :: 4,076
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 89
            rRNAs                             :: 10, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 10 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 19 of 69
            Pseudo Genes (incomplete)         :: 44 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 10 of 69
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1733
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13376389-rid12479423.denovo.353"
                     /strain="HB27"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="China: Hebei"
                     /collection_date="2017"
     gene            5..373
                     /locus_tag="KRL67_003896"
                     /pseudo
     CDS             5..373
                     /locus_tag="KRL67_003896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889008.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            661..979
                     /locus_tag="KRL67_003897"
                     /pseudo
     CDS             661..979
                     /locus_tag="KRL67_003897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897465.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          718 a          184 c          224 g          607 t
ORIGIN      
        1 ataattatat tatacaataa caatatataa gctaattaaa aaccttaaga agaacgatat
       61 tgacactact tgaattatat tatagaaaag ctgaaataaa taaaatagtt tgggctagaa
      121 taggttttaa tttatatctt tctaaaggag ttgcattaac tttaactgat gctggtatag
      181 ggatttcagg acttttggct ggtgctattc ctggtatagg acagtacttg ccacttatag
      241 ttagagtaat tacaatgtac cttgagtcaa atataacatc tggaaaaatc tttaaattta
      301 aaaaatacgt tataataaag gaggtgcttg tgtaaccaaa tactacttac aatctgtaga
      361 aatcaatagg tgataaaatg attataaaat atctactttt tgcaattgtt tcagcattaa
      421 ttttattatt ttgtaataaa gcattccaac taagtaaatt acacaagttc attatattgt
      481 ttgtttcatt ttttatacta acttttgtag catcattcat tagtagataa aaaataagat
      541 ggtggggtta acttaccatc tttttctcat ttaatatttg taatatttta gatactcttt
      601 atacttgtgt tactgttata ctttgattag gaaaagtaaa tttaacagga ggtatctgct
      661 atggattttg gaaatatcaa tcttatactt atcggaataa tagttataat aggaactaca
      721 ataatatatt taattaaacc taaaactgct ttttgtagta aaaaatattt taataaatta
      781 gaatcaatat atggaaatat agataagaaa aaaactgtta aactagaaat actttatcgc
      841 tatgtaacag gtcttgagta tatagcaata ggcttattta caaaaagatt agatattaca
      901 aataatatca ataatattag tagctactat aacagtaata ttatattact tagttagaaa
      961 gagatatata actatataaa atctaataga attaatatca aaagaaggta gcaactaatc
     1021 accagtaatg taaaataatt tgtttttcaa agttaataaa agtcttcttt atggcaatga
     1081 tttaaatata atcaaatatc gtaacggaga tttttttatg aataatttaa gaaaacaatt
     1141 aatcaaagaa attatataag atggagaact tataaaatct acagatataa gtagttttag
     1201 gagtaaacac agatgactta tttcaaagag acaatagcta caagttatcc tcattctgta
     1261 atacaaaaat gtttaataca ttagataaga aatactttaa atatgtttca tacaaggact
     1321 caaaagaatt aataagtgat tttaaatctg tatataggtc agttaatgaa taaaaagcct
     1381 tatcgacttt agataaaata gattaaaaat aatgtaaaca gtatacttca gaaattaaaa
     1441 cgtttggaaa taactggaat gttgtagcat cagcctttag ttttactaaa ataagtaata
     1501 tctataatat atattacaag tataatcgaa ggatttaata aatatttcaa aaatgtctca
     1561 aaaacaaagt ccagatttac atcaaacgct tcactcttaa agatattatt cttgacaagc
     1621 gaaaatgtga tgaaaaaatg gactatgaaa tataaaaatt gatataatat atacaatcat
     1681 ttattcatat cttttgatag tagaatataa ttttaaaaat tataccctaa aaa
//
LOCUS       DAEPXF010000346         1661 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain HB27
            SAMN13376389-rid12479423.denovo.354, whole genome shotgun sequence.
ACCESSION   DAEPXF010000346 DAEPXF010000000
VERSION     DAEPXF010000346.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13376389
            Sequence Read Archive: SRR10518432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1661)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1661)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001069624.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001069624.1
            Genome Coverage       :: 315x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/15/2021 01:54:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,165
            CDSs (total)                      :: 4,076
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 89
            rRNAs                             :: 10, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 10 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 19 of 69
            Pseudo Genes (incomplete)         :: 44 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 10 of 69
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1661
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13376389-rid12479423.denovo.354"
                     /strain="HB27"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="China: Hebei"
                     /collection_date="2017"
     gene            109..843
                     /locus_tag="KRL67_003898"
     CDS             109..843
                     /locus_tag="KRL67_003898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HBH1524701.1"
                     /translation="MKQNKYDDDKFFNKYSKMERSTNGLAGAGEWHALKKMLPDFKGK
                     RVLDLGCGFGWHCQYAVENGAISAVGIDISEKMLKEARNKTQLDNINYMCMPIEDINF
                     PKGSFDVVISSLAFHYIQSFEDICKNISSCLSNKGNFVFSVEHPVFTAYGSQEWYCDK
                     DGNNLHWPVDRYFEEGVRKSNFLGEEVIKYHKTLTTYLNTLIKTGFEILEIIEPQPEE
                     KLLDTIPGMKDELRRPMMLLVSAKKR"
     gene            complement(986..1477)
                     /locus_tag="KRL67_003899"
     CDS             complement(986..1477)
                     /locus_tag="KRL67_003899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA synthetase associated
                     domain-containing protein"
                     /protein_id="HBH1524702.1"
                     /translation="MNNQQQEVYKKLDELSIPYEAVNHPPVYTIEEMEELKTLHMEFV
                     VKNLFLRDAKGKEHFLVVLGKDKKADLKDIRQQIDSKPLSFASEERLQKYLKLTKGAV
                     TPFGILNDKEAVVKVVFDKDLLKMDKIGIHPNDNTATVFLKFEDMKKLIEENGNEIYY
                     VEV"
BASE COUNT          613 a          205 c          243 g          600 t
ORIGIN      
        1 aatttgttta tagaaacttt tattatttca cgtaaaagtg ataaaagctt atattttgta
       61 tttaatagag tgttgctaag tgaatatatt taaaatggag gaaattttat gaaacaaaat
      121 aaatatgatg atgataaatt ttttaataaa tacagtaaaa tggaacgttc tacaaatgga
      181 ttagcaggtg ctggagaatg gcatgcttta aaaaaaatgt tacctgattt caaaggaaaa
      241 agagttttag atttaggatg tggatttgga tggcattgtc aatatgcagt tgagaatgga
      301 gcaatttctg ctgttggaat tgatatttca gaaaaaatgt taaaagaagc tagaaacaaa
      361 acgcaacttg ataatataaa ttacatgtgt atgcctattg aagatattaa ttttcctaaa
      421 ggtagttttg atgtagttat tagttctttg gcatttcatt atattcaatc atttgaagat
      481 atatgtaaga atataagtag ttgtctttca aataaaggta attttgtatt ttctgttgaa
      541 catcctgtat ttactgcata tggaagtcaa gagtggtact gtgataaaga tggcaataat
      601 ctacactggc cagtagatag atattttgaa gaaggagtac gtaagtcaaa ttttctagga
      661 gaagaggtta taaaatatca taaaacatta acaacttatt taaatacgtt aattaaaaca
      721 ggatttgaaa tattagaaat tattgaacct caaccagaag aaaaactgtt agatacaatt
      781 cctggtatga aagatgagct acgtagacct atgatgctac ttgtatctgc aaaaaaaaga
      841 taagcaaata taaataatca ttattgtaga acgaaagttg tagaaaacaa ttacactaat
      901 agatatgatt ataccagtgg tattaattac accagtagta aggaggcaaa agcattcatt
      961 gcttttgcca ttttttaagt tgcaattata cttccacgta gtatatttca tttccatttt
     1021 cttctataag ctttttcata tcttcaaatt ttaagaatac agtagcagta ttatcatttg
     1081 gatgtatacc tattttatcc atttttaata aatccttatc aaatacaact tttacaacag
     1141 cctctttatc atttaatata ccaaaaggag tcacagctcc tttagtaagt ttcaagtatt
     1201 tttgaagtct ttcttctgat gcaaaactaa gaggtttact atcaatttgt tgacgaatat
     1261 cttttaaatc tgcttttttg tctttaccta atacaactaa aaaatgttct tttcctttag
     1321 catcacgaag aaataggttt tttacaacaa attccatatg taatgttttt aattcttcca
     1381 tttcttcaat tgtataaact ggtggatgat ttacagcctc ataaggtatg cttaactcat
     1441 ctaatttttt atatacttcc tgctgttgat tgttcatgat ttcctcccat tagaaattta
     1501 ttttatatat aaataatatt atcacagaaa actttaaaag aaaacgtttt atataatata
     1561 gacaatagat tatattttta taaagaagca agaatactca ataaaactta aagaaattaa
     1621 acaatttcta aaaatacctt gaaaatagaa ccttatttgc c
//
LOCUS       DAEPXF010000347         1626 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain HB27
            SAMN13376389-rid12479423.denovo.355, whole genome shotgun sequence.
ACCESSION   DAEPXF010000347 DAEPXF010000000
VERSION     DAEPXF010000347.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13376389
            Sequence Read Archive: SRR10518432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1626)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1626)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001069624.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001069624.1
            Genome Coverage       :: 315x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/15/2021 01:54:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,165
            CDSs (total)                      :: 4,076
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 89
            rRNAs                             :: 10, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 10 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 19 of 69
            Pseudo Genes (incomplete)         :: 44 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 10 of 69
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1626
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13376389-rid12479423.denovo.355"
                     /strain="HB27"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="China: Hebei"
                     /collection_date="2017"
     gene            215..1486
                     /gene="serS"
                     /locus_tag="KRL67_003900"
     CDS             215..1486
                     /gene="serS"
                     /locus_tag="KRL67_003900"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000884181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HBH1524703.1"
                     /translation="MLDIKRIRENLDGIKKAMERRGEKDFNLDEVVKLDDERRRILQE
                     VEVMKNELNTASKNIPNLIKEGKDVENEKIKLKQLSDKIKIIDQNLKEVEDKMEYLLM
                     RIPNVPHPEVPQGETDEDNVEVRTWGKTTTFDFESKAHWEIGTELGILDFETASKITG
                     SRFTLYKGLGARLERALLNFYLDTNTKVNGYTEVIPPFMANRNSFLGTGQLPKFEEDM
                     FKIEGLDYFMIPTSEVPLTNIHANEILKFEQLPINYTAYTPCFRSEAGSAGRDTRGLV
                     RQHQFNKVEMVKIVAPEESYNELEKLTNNAETMLQLLNLPYRVVKICTGDLGFTASFK
                     YDVEVWMPSYNRYVEISSCSNCEDFQARRAGIRFKRDKDSKAEYVHTLNGSGLAIGRS
                     VAAILENYQQEDGSVIVPEVLRPYMGVSVIK"
BASE COUNT          688 a          167 c          296 g          475 t
ORIGIN      
        1 aagttaaatc tagcaatatt ctcctaaaat taaattatat ttatttaatt aaatataatt
       61 tgacaataga caaaaaagtg agtaaaatat aaataaaata aaaataacag gttcggataa
      121 agaaaaaggt ttcttaacct caagcataaa gtatattatc taagttattt gagattaaga
      181 gataaatcca atcttaggta ttgaggagga aaaaatgctt gatataaaaa gaataagaga
      241 aaatttagat ggtataaaaa aagcaatgga aagaaggggc gaaaaagact ttaaccttga
      301 tgaagtagtt aagttagatg atgaaagaag aaggattctt caagaagttg aagttatgaa
      361 aaatgaatta aatacagcat ctaaaaatat accaaatctt ataaaagaag gtaaggatgt
      421 agaaaatgaa aagattaaac taaaacagtt atctgataaa attaagataa tagaccaaaa
      481 tcttaaagaa gtagaagaca agatggaata cctattaatg agaataccaa atgtaccaca
      541 tccagaagta ccacaaggtg aaactgatga agataatgtt gaggtaagaa cttggggaaa
      601 gactactacc tttgattttg aatctaaggc tcattgggaa attggaacag aattaggtat
      661 acttgatttt gaaacagcta gtaaaataac tggttcaagg tttactcttt acaaaggatt
      721 aggtgctaga ttagagagag cattattaaa tttttacttg gacacaaata caaaagtaaa
      781 tggatataca gaagttattc caccatttat ggcaaataga aatagttttt taggtacagg
      841 acagttacct aagtttgaag aagatatgtt taaaatagaa ggtctggact attttatgat
      901 tccaacatca gaagttcctt tgactaatat acatgctaat gaaatactta aatttgaaca
      961 gttaccaata aattatacag cttatactcc atgttttagg tcagaagctg gttctgctgg
     1021 aagagataca agaggtttgg taagacagca tcagtttaat aaagtagaaa tggttaaaat
     1081 agttgcacca gaagaatctt acaatgagtt agaaaagctt actaacaatg cggaaactat
     1141 gctacaatta ttgaatctgc cttatagagt agtaaagata tgtacaggtg atttaggatt
     1201 tacggcttct tttaaatatg atgtagaagt ttggatgcca agttacaata ggtatgttga
     1261 gatttcatct tgttcaaact gcgaagattt ccaagctaga agagcaggaa taagatttaa
     1321 aagagataaa gattctaaag ctgaatatgt acacacatta aatggctctg gtctagctat
     1381 tggaagaagt gttgcagcta tattagaaaa ttatcaacaa gaagatggtt ccgtgattgt
     1441 tcctgaagtt ttaagaccat atatgggagt ttctgttatt aaataagagt taaattatgt
     1501 atttttaagt aatacaataa tatagaggta ataaaaaaat aattaagata gaagttgtac
     1561 aataagatag aagtaataaa aaaattaata taaaaatgat acaataataa tagaagtaat
     1621 aaaaaa
//
LOCUS       DAEPXF010000348         1623 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain HB27
            SAMN13376389-rid12479423.denovo.356, whole genome shotgun sequence.
ACCESSION   DAEPXF010000348 DAEPXF010000000
VERSION     DAEPXF010000348.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13376389
            Sequence Read Archive: SRR10518432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1623)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1623)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001069624.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001069624.1
            Genome Coverage       :: 315x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/15/2021 01:54:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,165
            CDSs (total)                      :: 4,076
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 89
            rRNAs                             :: 10, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 10 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 19 of 69
            Pseudo Genes (incomplete)         :: 44 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 10 of 69
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1623
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13376389-rid12479423.denovo.356"
                     /strain="HB27"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="China: Hebei"
                     /collection_date="2017"
     gene            166..1569
                     /locus_tag="KRL67_003901"
     CDS             166..1569
                     /locus_tag="KRL67_003901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp9"
                     /protein_id="HBH1524704.1"
                     /translation="MKNSKKILAIGLTLFLVMVNTPMVNALTSVEQIKGNDRYETAAK
                     IADKQNYNTAILINSDNSLADGLSASGLAGALNAPILMTKQNQIPNTTMERLNKAKTV
                     YIIGSESTISKNVENQLLSKKKVVQRIFGENRFDTSIKIAEKIKEIKPIDKVIIANGF
                     TGEADAISASPVAARDGVPIILTDGNSVGFDTTGLKSYALGSSEIISDELVKSTNSIR
                     LGGTDRFETNKIVIQEFYKNSKEFYLSKGLQLTDALAASTIAKNAPVVLVENGSNKSI
                     LSGADKLTVLGGINQNVIKQCINQASPNQQGLYYNPNDRAFKERIKGKVYALTKQYRK
                     ENGVRALSVASRLEGLANDWSNLMANKKTLSHTINGKNSYSTFLKYLDWSEIKPGYIA
                     VQGENIIKYKIPDKPVYTNRDADDIGNFIFNEWKTNPEEGTNMLHKGYEIMGFGIAIT
                     GDKNLYATHEFYGRYKE"
BASE COUNT          677 a          180 c          315 g          451 t
ORIGIN      
        1 tgttttgaat ttatagagaa taaaaacttt aggtatattg tttgagttgt atggataact
       61 atggtatcat atataaaata tggtaacaaa attgtaactt ttgattaaaa ataagtatga
      121 gtactaagaa aaaatgtgaa aagtacattg ggggtagaaa ataatatgaa gaatagtaaa
      181 aagatattag ctataggact tacactattt ttagtaatgg taaatactcc tatggtaaat
      241 gcgttgacat cagttgagca aataaaaggg aatgacagat atgagacagc agcaaaaata
      301 gcagataagc aaaactacaa tacagcaata ctaatcaact cagataatag cttagcagat
      361 ggtctaagtg caagtggttt agctggagcc ttaaatgcgc ctattttgat gacaaagcaa
      421 aaccagattc caaacacaac tatggaaaga ttaaacaaag caaagactgt gtatataata
      481 ggctcagaat caacaataag taaaaatgtt gagaatcagt tactatcaaa aaagaaggtt
      541 gtacaaagaa tatttggtga aaatagattt gatacaagta taaaaatagc tgaaaaaatc
      601 aaagaaatta agccgataga taaagtaatt atagccaatg gatttacagg agaagcagat
      661 gcgataagtg catcaccagt agctgctaga gatggagtac ctataatact tacagatgga
      721 aatagtgtgg gatttgacac gacaggctta aagagttatg cgcttggttc tagtgaaata
      781 atcagcgacg aattagttaa gagcacaaat tctattagat tgggtggcac agatagattt
      841 gaaactaata agatagttat acaagagttt tataaaaata gtaaagaatt ttatctaagt
      901 aaaggcttac aattaacaga cgctttagct gcctctacaa tagcaaaaaa tgctcctgta
      961 gttttagttg aaaatggcag taacaagagt attttaagtg gtgcagacaa attgactgta
     1021 ctaggaggca taaatcagaa tgttatcaag caatgtataa atcaagcttc accaaatcaa
     1081 caaggtttat attacaatcc aaatgataga gcatttaaag agagaataaa gggtaaagta
     1141 tatgctctta caaagcagta tagaaaagag aatggagtaa gagcgttatc tgtagcaagt
     1201 agattagagg gtctagcaaa tgattggtct aatttaatgg caaataagaa gactttatct
     1261 catacgataa atggaaaaaa ttcatattct acttttttga aatacttaga ttggagtgag
     1321 attaaacctg gatatatagc agttcaaggt gaaaatataa ttaaatataa aattccagat
     1381 aaacctgtat atacaaatag agatgcagat gatataggaa attttatttt taatgagtgg
     1441 aagacaaatc cagaagaagg cactaatatg ttgcataaag ggtatgaaat aatgggattt
     1501 ggtatagcta taacagggga taagaatcta tatgctacac atgaatttta tggaagatat
     1561 aaagaataag aaaatataca caaaattatg cttgtaagga agctatataa ataattaaat
     1621 tat
//
LOCUS       DAEPXF010000349         1608 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain HB27
            SAMN13376389-rid12479423.denovo.357, whole genome shotgun sequence.
ACCESSION   DAEPXF010000349 DAEPXF010000000
VERSION     DAEPXF010000349.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13376389
            Sequence Read Archive: SRR10518432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1608)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1608)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001069624.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001069624.1
            Genome Coverage       :: 315x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/15/2021 01:54:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,165
            CDSs (total)                      :: 4,076
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 89
            rRNAs                             :: 10, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 10 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 19 of 69
            Pseudo Genes (incomplete)         :: 44 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 10 of 69
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1608
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13376389-rid12479423.denovo.357"
                     /strain="HB27"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="China: Hebei"
                     /collection_date="2017"
     gene            146..1582
                     /locus_tag="KRL67_003902"
     CDS             146..1582
                     /locus_tag="KRL67_003902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp28"
                     /protein_id="HBH1524705.1"
                     /translation="MKFYKRILALTLSISFVFANIQSVNAISSVEKIQGKNKYEIAGK
                     IADKNAYKTAILINTSNSIADGLSASGLAGALNAPILLTEKNTIPTETYARLKNVSKV
                     YIIGGTYSISTSVENSLKSKKMKVVRIKGNDRIKTSYNVAKEINSIKKVNTVMLTNAY
                     KGEADAISIASVAARDKAPIILTNGQSIPFSTSGLKSYAIGGTASMSTTLVNNTKSTR
                     LGGSTRFETNKAIINKFYKDAREFYIAGAYELTNALVGSSLSKHGPMVLVNDGSNKSI
                     LKNAKKITSIGYIDSNIVQQCLNITNGIGDINTGVVKNVKPTTKTIKDGMYKVGKDIS
                     AGEYLITSNSGSYASYYEVTSDSTGNADSILSNDIFSGTRYITLKNGQYIKIEDSTMI
                     LAKYAKAQKAKNGKFGNGMYKIGLEIPAGEHIIMSNSSDAYYEVRNDSLGNADGIVTN
                     DTFSGRRYITVEEGQYLILNDCYLIENE"
BASE COUNT          652 a          172 c          279 g          505 t
ORIGIN      
        1 tagctaaatg aataattgca ttgatattta ttagtaacat ggtctaatat agtttgcaat
       61 tttacataaa tttgttaagt acataaccaa tttaagagta tgtatttata tattaaaaat
      121 ttattatgag ggggacaatt ttattatgaa attctataaa agaatattag cattgacttt
      181 aagtatttct tttgtatttg caaatattca atcagtaaat gcaatatcat ctgtagaaaa
      241 aatacaagga aaaaataagt atgaaatcgc aggtaaaata gcggataaaa atgcatataa
      301 aactgctata ttaattaata ctagtaactc tatcgcagat ggattaagtg caagtggact
      361 agcaggagct ttaaatgccc ctatactatt aactgaaaaa aatactatac caactgagac
      421 atatgcaaga ttaaaaaatg tatcaaaagt ttatataatt ggaggaacat attctattag
      481 tacatctgtc gaaaattcat taaaatctaa gaaaatgaaa gttgtaagaa ttaagggaaa
      541 tgacagaata aaaacaagtt ataatgttgc aaaagaaata aattcgataa aaaaagttaa
      601 tactgttatg ttaaccaatg catataaagg agaagcagat gctataagta ttgcatctgt
      661 agcagcaaga gataaagcac caataattct tactaatgga caaagtatac ctttttcaac
      721 tagtggttta aagtcttatg caataggagg aacagcttca atgagtacca ctttagtaaa
      781 caatactaaa tctactagac taggggggag tactcgtttt gaaactaata aagcaataat
      841 aaataagttt tataaggatg cgagagagtt ttatatagct ggagcttatg aattaacaaa
      901 tgcacttgta ggttcttctt tatcaaaaca tggtccaatg gttttagtta atgatggtag
      961 caataaaagc attttaaaaa atgctaagaa gataacttct ataggatata tagattcaaa
     1021 tatagtacaa cagtgtttaa atatcacaaa tggaattgga gatattaata ctggggttgt
     1081 aaaaaatgtt aagcctacta caaagactat aaaagatgga atgtataagg ttggaaaaga
     1141 tatctcagca ggtgaatact taataacttc aaactcagga agttatgctt cttattatga
     1201 agttacaagt gatagcacag gtaatgctga tagtatactt tcaaatgata tttttagtgg
     1261 gactagatat atcaccttaa agaatggtca atatattaaa attgaggatt caacaatgat
     1321 attagcaaag tatgctaaag cccagaaggc caagaatggt aagtttggaa atggtatgta
     1381 taaaattgga ttagaaattc ctgctggaga acatattata atgtcaaatt cttctgatgc
     1441 atattatgaa gttagaaatg atagcttagg taatgcagat ggaatagtta ctaatgatac
     1501 tttttcagga agaagataca taacggttga ggaaggtcaa tatttaatat taaatgattg
     1561 ttatttaatt gaaaatgaat agatgtaatt gtatttaata gtaaatca
//
LOCUS       DAEPXF010000350         1557 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain HB27
            SAMN13376389-rid12479423.denovo.358, whole genome shotgun sequence.
ACCESSION   DAEPXF010000350 DAEPXF010000000
VERSION     DAEPXF010000350.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13376389
            Sequence Read Archive: SRR10518432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1557)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1557)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001069624.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001069624.1
            Genome Coverage       :: 315x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/15/2021 01:54:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,165
            CDSs (total)                      :: 4,076
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 89
            rRNAs                             :: 10, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 10 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 19 of 69
            Pseudo Genes (incomplete)         :: 44 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 10 of 69
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1557
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13376389-rid12479423.denovo.358"
                     /strain="HB27"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="China: Hebei"
                     /collection_date="2017"
     gene            complement(461..850)
                     /locus_tag="KRL67_003903"
     CDS             complement(461..850)
                     /locus_tag="KRL67_003903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HBH1524706.1"
                     /translation="MTIKKLPQSELKIMKFIWKSDSKVTSRDIVLGMEQKYQWKQTTT
                     LTLLSRLVVKRFLNSQKIDKYTHYEVLIKEKEYIGVETRDFFRNIHDSSIKSLLLSLH
                     ENINLSKDDILLIEEWIKNLKEEEKDV"
BASE COUNT          503 a          266 c           97 g          691 t
ORIGIN      
        1 cactatcttt tattaatctt ttgtttttac tttttcaata tcttctatta atcttttatt
       61 tttatttttt caatatcttc attaattcct atttatttta tcatcttaaa tacgaaaaca
      121 ctgtgtcata tttgcaaaat atatattgtc atctatatta aagcttcaca atttatattt
      181 aaaaagttac tttaattctc tacagtttat atttaaaaat tctctttaat cacactacct
      241 ctatttaaaa agctacctta attatactta taagtaaagg tagcttttta aatttaatta
      301 aatagaatat ccactatcaa tttatactta atttatttag attcacatta tagtatcata
      361 atccacttat aatcatttgt catattactt aagtagacta atatattaca taaatacact
      421 tacatacact ttatattgat gctataagaa atacgtttgc ttatacgtct ttctcctctt
      481 cttttagatt ttttatccac tcttcaataa gtaagatatc atctttgctc aaattaatat
      541 tttcatgtaa tgacaacaat aaacttttta tagaagaatc atgaatattt ctaaaaaaat
      601 ctcttgtctc aactcctatg tattcctttt ctttaattaa aacttcataa tgtgtatatt
      661 tatctatctt ttgagaattt agaaatcttt ttactactaa tctagataaa agagtcaaag
      721 ttgtcgtttg tttccattga tatttttgct ccattcctaa aacaatatct ctagatgtta
      781 ctttggaatc tgatttccat ataaatttca ttatctttaa ttctgattgt ggcaactttt
      841 ttatagtcat gataaaacct tgctactcag aatatagaca agcaatatcc ataataaaat
      901 ttttcgatta aatacatctt atacggattt atataagctc agacttataa gatatttttc
      961 aatcttaatc ctaaatttag ctatttgtac acgttaaata tatataaata atgtcaaaat
     1021 tctctcgtat tatattgctt aattttatga aatattttgt gctcagctat ctattttgga
     1081 tacttgtcca gtagctctcc tttcttttaa actctttgat tttaaatatt tgtatttcag
     1141 taaatcccac ttcctaaatt tcttatttac tacaacttat tactaaactt cctactttct
     1201 aaatttttta tttactacaa cttattacta acttcctact ttctaaattt cttacttgct
     1261 acaaattatt actaaacttc ctactttcta aatttcttac ttactactac ttatcactaa
     1321 actttccact ttctaaattt cttactcact atgacttatt actaaacttt ccactttcta
     1381 aatttcttac ttactaccac ttatcactaa acttcctact ttctaaattt cttactcact
     1441 atgacttatt actaaacttc ctactttcta aatttcttac ttgctataac ttactactaa
     1501 actttttact tcttaaattt cttacttctt aagaataatg ttaattttaa ttttaag
//
LOCUS       DAEPXF010000351         1551 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain HB27
            SAMN13376389-rid12479423.denovo.359, whole genome shotgun sequence.
ACCESSION   DAEPXF010000351 DAEPXF010000000
VERSION     DAEPXF010000351.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13376389
            Sequence Read Archive: SRR10518432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1551)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1551)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001069624.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001069624.1
            Genome Coverage       :: 315x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/15/2021 01:54:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,165
            CDSs (total)                      :: 4,076
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 89
            rRNAs                             :: 10, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 10 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 19 of 69
            Pseudo Genes (incomplete)         :: 44 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 10 of 69
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1551
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13376389-rid12479423.denovo.359"
                     /strain="HB27"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="China: Hebei"
                     /collection_date="2017"
     gene            complement(923..1399)
                     /locus_tag="KRL67_003904"
     CDS             complement(923..1399)
                     /locus_tag="KRL67_003904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000469298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBH1524707.1"
                     /translation="MFIDSEKRLKQLSDEAKKNTEDLEEAKKNSRFTQVSPKGWERVR
                     ELLKDSQGISALKLYSFLAEHIDPTCGAVVADQQFLAEKLGVSRSTIIRWLNYLESKN
                     ALVRIPVAGKVCAYALDPHEVWKGYNTTKNHAAFVTKTLVNKDGDIQRRIMAMFSN"
BASE COUNT          355 a          413 c          386 g          397 t
ORIGIN      
        1 aaaaaaacaa gcacttacaa atcggcgtaa aagatctatt ctaggcaagg gtgttgcgtg
       61 caacgcaacg ccctttgggg gtgtcggggc gaagccctga cccagtcacg tagcgatagc
      121 ggagtgtata ctggcttact cctctgcaag cctcgtcagc cgtgattttg cacgatttcg
      181 cgctctccac gttcaacagc tcgatcatgt ttttgccttc cgttcaccgc taaaacgccc
      241 caaaaatcgg gacaggatgt gcaaatgtaa taccgtcacc cgcgacgata ttccgtttgc
      301 catctggtca gggcttcgcc ccgacacccc gcatcagtgc gtacgtgctc aatgtggttt
      361 ccggttctct tccgcttcct cgcccactcg ctcactacgt tcggtcgctg gggctgcggc
      421 gagcgggatc acctctcgca tgttgtcggt gatcccggtg ttgttctttt ggggatgagt
      481 cgccagatag cccctcaaac gcgctcaaaa gctctcagac gcacgaaacg ggttttagat
      541 gcccgccagg gcatggaagg cgcgaaaaac gcttacagag gctctcagga ggtcaagcgc
      601 gccgataccc ctaaccaggg gctttcaggt cgccaggaac cgcgccagcc ccctgcaaag
      661 cccatttgca cggaacaggc agccatgcgg ttggtaggtg gtcgtagacc gtcggtgttg
      721 cagcgatggg ggtctggggt tctccccaga cggtttccgg tagcgctcaa ggcgctgccg
      781 gaaccgtggc ggctcggttt tttctggaga acggggagca aaaacacgca aaggattttg
      841 aggcggcaaa agagcaatgc aggatagaca atcatggtca gaacgttaac acgtagcccc
      901 tgattgtccg cctgccgcta gctcaatttg aaaacatggc catgattcgg cgctgaatat
      961 caccgtcttt gttgaccagt gttttagtga caaacgctgc atggtttttc gtagtgttgt
     1021 atcccttcca gacttcatgt ggatcgaggg catacgcaca aaccttacca gcaacgggga
     1081 ttctaactaa tgcatttttt gattctaagt aattgagcca ccgaataatt gtgcttctgc
     1141 taactccaag tttttcagct aggaattgct gatccgcaac gacagcgcca cacgtaggat
     1201 cgatatgctc cgctaaaaat gagtacagct tcagtgctga tatgccttgg ctatccttca
     1261 gcagctctcg aacacgttcc caaccttttg gggatacctg tgtaaacctt gaatttttct
     1321 ttgcttcttc gagatcctcg gtgtttttct ttgcctcatc tgaaagttgt ttcagtcgtt
     1381 tttctgaatc aataaacatg attttttccc tctgaaaatc ccatgcacta acgttggttc
     1441 aaatctaagc catcatgaca catctgtgca acaccttgtt gcgtgttgtc gcatccagga
     1501 tgcgacaacg ttgtgtcatc ctggatgcga caggtaaata aaaaacacac t
//
LOCUS       DAEPXF010000352         1546 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain HB27
            SAMN13376389-rid12479423.denovo.360, whole genome shotgun sequence.
ACCESSION   DAEPXF010000352 DAEPXF010000000
VERSION     DAEPXF010000352.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13376389
            Sequence Read Archive: SRR10518432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1546)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1546)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001069624.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001069624.1
            Genome Coverage       :: 315x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/15/2021 01:54:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,165
            CDSs (total)                      :: 4,076
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 89
            rRNAs                             :: 10, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 10 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 19 of 69
            Pseudo Genes (incomplete)         :: 44 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 10 of 69
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1546
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13376389-rid12479423.denovo.360"
                     /strain="HB27"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="China: Hebei"
                     /collection_date="2017"
     gene            18..473
                     /locus_tag="KRL67_003905"
     CDS             18..473
                     /locus_tag="KRL67_003905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009170763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBH1524708.1"
                     /translation="MRELKEDNIKNALDLIWNVFEEFESPDYCDEGITTFHQFIEYKS
                     IIEKVKEKTLFFWGCFDSKKLIGVLAIRDLNHICMLFVDKNYHKHGVAKKLMTTGIQY
                     CICNKFNLTEITVNSSPYAVEVYHKLGFKDTAVEQIVDSIRFTPMKLLI"
     gene            complement(832..1389)
                     /locus_tag="KRL67_003906"
     CDS             complement(832..1389)
                     /locus_tag="KRL67_003906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBH1524709.1"
                     /translation="MRVSREIVIQATSDIVNKDGLNKVSLKVIAEKLGIRTPSLYNHI
                     ESLDDLLLEVAHKGMREMNNQMIKAVIGNFGDTAIKCISIAYFNFIISNPGVYETIQW
                     ATWHGNNETLEIFDEYKSLLEKLILSCNLNTKNTGEVLNLIMSVLHGYSTLQLGKSLL
                     SKEEAIKGLADSIDTVLLGIHKKYD"
BASE COUNT          561 a          201 c          171 g          613 t
ORIGIN      
        1 tgttcaaaga atacttaata agagaattaa aagaagataa tattaaaaat gcattagact
       61 taatctggaa cgtatttgaa gaatttgaat ctccagatta ttgtgatgaa ggaattacga
      121 catttcatca atttatagaa tataaatcta ttattgaaaa agtcaaagaa aaaacactct
      181 tcttttgggg ttgttttgac tctaaaaaac ttattggagt cttagctatt agagatttaa
      241 atcatatatg tatgttattt gtagataaaa actatcataa acatggtgtt gcaaaaaaat
      301 taatgactac aggtatacaa tactgtattt gtaataaatt taatttaact gaaataactg
      361 taaattcatc accttatgct gttgaagttt atcataaatt aggatttaaa gatactgctg
      421 ttgagcaaat tgttgacagt atacgtttta cacctatgaa attgttaata taataaatta
      481 aataaaaagc aaagtctctt aaatatttta tagtgacttt gcttttaaac cgagatttaa
      541 aaataatatt atctttttta ttttttaata atatacttta aaaacattac taatatgaac
      601 tctaatttat ttttgatatc atttttgact aacatataaa ttaattcaat tattaagcat
      661 aaactatgtt atttctctta tttgtaagca atattggtca tttgaaaata aatattctta
      721 aaatatactg ttctcctgtg gattttcctt tggtataaac gataaaattt taatttacat
      781 gccttgtaaa gaagtgttca ataaataagt taaaataatt tatttacttc cttaatcata
      841 ttttttatga atacctaata acacagtatc aatagaatcg gctaaacctt ttatagcttc
      901 ttctttactc aaaagtgatt tcccaagttg caaagtcgaa taaccatgaa gtacactcat
      961 tattaagttt aatacttcac ctgtattctt tgtatttaga ttacaagaaa gaataagttt
     1021 ttctaataat gatttatatt catcaaagat ttctaatgtt tcattatttc catgccaagt
     1081 agcccactga attgtttcat aaacaccagg attagaaatt ataaaattaa agtatgctat
     1141 acttatacac tttatagctg tatctccaaa atttccaata accgccttta tcatttgatt
     1201 attcatctct ctcattcctt tatgagctac ttcaagtaat aaatcatcta gactttctat
     1261 atgattgtac aaagatggtg tacgtattcc tagtttttct gcaataactt ttaaagaaac
     1321 tttgttaagc ccatctttat taactatatc tgatgtagct tgtataacta tttctctact
     1381 gacacgcatg attttccctc tttcatttaa aattgattac tttattagtt tagctaatct
     1441 aattagtttc gtcaattatt gtaatttata aaattcttta tactattata aaatcttgac
     1501 agtttaatta taatttaata taatgctaat tgaattagtt ttaata
//
LOCUS       DAEPXF010000353         1494 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain HB27
            SAMN13376389-rid12479423.denovo.361, whole genome shotgun sequence.
ACCESSION   DAEPXF010000353 DAEPXF010000000
VERSION     DAEPXF010000353.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13376389
            Sequence Read Archive: SRR10518432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1494)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1494)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001069624.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001069624.1
            Genome Coverage       :: 315x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/15/2021 01:54:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,165
            CDSs (total)                      :: 4,076
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 89
            rRNAs                             :: 10, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 10 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 19 of 69
            Pseudo Genes (incomplete)         :: 44 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 10 of 69
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1494
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13376389-rid12479423.denovo.361"
                     /strain="HB27"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="China: Hebei"
                     /collection_date="2017"
     gene            94..1433
                     /locus_tag="KRL67_003907"
                     /pseudo
     CDS             94..1433
                     /locus_tag="KRL67_003907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888366.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
BASE COUNT          579 a          117 c          236 g          562 t
ORIGIN      
        1 acaatctatt tactacacgt gtagagtcgt atataattaa attatacata aaatataaaa
       61 aaataattta gtaatacgtt aggagaaagt attatgataa agtcaagctt attttggaat
      121 attttcattg cgatatcctt tatgatagaa tggtttcttt gtaagaaaat gctagattat
      181 acaagtgaga aaaggatttc taacaaaaaa taaacttttg tatggtaggt attattgttt
      241 ttgctgacat aatatatttt tttgatgtac ctccaaatat aagagtaatt atatctataa
      301 taattactat tgcattttat aaaatctcat atgatgtgag gtttataaag tctgtattaa
      361 tatccttaat ttattggatg ttactaatcg ccacagatgc tttgagtata tctttaacag
      421 tttggcttaa ttctataaat gacatgagta gtatagggac gggagatata tacagaattg
      481 agtctataat attgggaaag atacttttga ttgtgtcttt gtttttatat agagccttaa
      541 agataaaact taatatagaa ataaataaaa ggacattatt ttatatagtt attcctatag
      601 tcgctaatat agcatctttt tttgtcattt ttgaatacat atttaatcca gggtctggta
      661 gcttaataca taatttccaa ttcgttatga tttctgttgt attacttgta tcaagtataa
      721 ctttagtttt aattgctaag aaattacagc aatataatac tttagttgta gaaaacaata
      781 tgattaaaaa tactttgaag tatgaaaatg agtattatga agatatagaa aagaaatatt
      841 taaaaactag attactaagt catgatatga aaaatcatat attgtgtata aattctatga
      901 ttaagaaagg tattgatgtt acttcttatt taaataattt acaggtagaa attcagagta
      961 atgacttgtt atttaatact ggtaatttta tattagatgc tatattaagt gaaaagaaag
     1021 aagtttgtga taagaatgat atagaactaa aaataggaat aaattttagt tgttgtgaag
     1081 ctgttgaatt agtagatata tgtcatatat tttcaaatat tttagacaat gcaattgagg
     1141 catgcaataa aattgatagt ggaaatagag ttatttgtgt taaaggtgat gttattcaaa
     1201 atttttatct aataagagtt gaaaatacta agattaataa gattattact aaaaatggag
     1261 atattttgac agataaaaaa ggttctcttc atggacttgg aataaaaagt gtaaaaaact
     1321 ctgtaaataa gtatggtggt aatgttgtaa ttgaatatga tgataaaaaa tttgtagtta
     1381 aaattgctat tccacttaga aatgtgaaga aagcagttaa tcatgtgatt taatgtatat
     1441 aaatttgatg tgtagaaatt taatatataa gaattaatgt ataagaattt aata
//
LOCUS       DAEPXF010000354         1459 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain HB27
            SAMN13376389-rid12479423.denovo.362, whole genome shotgun sequence.
ACCESSION   DAEPXF010000354 DAEPXF010000000
VERSION     DAEPXF010000354.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13376389
            Sequence Read Archive: SRR10518432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1459)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1459)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001069624.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001069624.1
            Genome Coverage       :: 315x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/15/2021 01:54:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,165
            CDSs (total)                      :: 4,076
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 89
            rRNAs                             :: 10, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 10 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 19 of 69
            Pseudo Genes (incomplete)         :: 44 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 10 of 69
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1459
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13376389-rid12479423.denovo.362"
                     /strain="HB27"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="China: Hebei"
                     /collection_date="2017"
     gene            <1..643
                     /locus_tag="KRL67_003908"
     CDS             <1..643
                     /locus_tag="KRL67_003908"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH1524710.1"
                     /translation="NNTRINESIVKKNETVGKDTYLSNIMKQKQELNDRIRDLKDRQE
                     IYTKKINEAIKNLYKSEIRETTNNFSNIEIGIKNRIIEDKNKSTMLDENSTYLNTNDE
                     KESLIAKEFNEKVEEEILNDEKLEELEQKKDYKEDSNKKETVSEDLSLVGKTREELEN
                     MLKNFINLTQEEIMKLESRIEKLDKDAEEYKQNSKTNIFDKTDEQKKHINVLV"
     gene            687..1235
                     /locus_tag="KRL67_003909"
     CDS             687..1235
                     /locus_tag="KRL67_003909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH1524711.1"
                     /translation="MEGLGFYVAALASGSYQRIGFGKLNPIEVIADGDWISYKQAQDT
                     LTVIRNFLNSFDWRNASEMERANRAAKLVTEAKYADSKYCNIVYGNLVDKRGVCGSFA
                     SSFHLLTRLMGMDSLSILNPSLNHAWNYIQIDGKWYRFDGSEISAFGGSLDFDYRKLK
                     DATREKTTYYDAKALSILGFNQ"
BASE COUNT          638 a          133 c          245 g          443 t
ORIGIN      
        1 aaataataca agaataaatg aaagtatagt taagaaaaat gaaactgtag gaaaagacac
       61 ttatttatct aatattatga aacaaaagca agaacttaat gatagaatta gagatttaaa
      121 agatagacaa gagatttata ctaaaaaaat aaatgaagca attaagaact tatataaatc
      181 agaaataaga gaaacaacta ataacttttc taatatagaa ataggtatta aaaatagaat
      241 tatagaagat aaaaataaaa gtacaatgtt agatgaaaat tcaacttatc taaatacaaa
      301 tgatgaaaaa gaatctttaa tcgctaaaga atttaatgaa aaagttgaag aagaaatatt
      361 aaatgatgaa aaattagaag agttagaaca aaaaaaggat tataaagagg attctaataa
      421 aaaagagacg gtatctgagg acttatcctt agtaggtaaa actcgtgaag agcttgaaaa
      481 tatgcttaaa aattttataa atttaacaca agaagaaata atgaaacttg agtcgagaat
      541 agaaaagtta gataaagatg ctgaagaata caaacaaaat tcaaagacta atatatttga
      601 taaaacagat gaacaaaaaa aacatataaa tgtactggtt taaacaatat gttttaaaaa
      661 aataagttta ttatataaat aagggtatag aaggtctagg attttatgtg gcagcattag
      721 cgagtggctc atatcagaga ataggctttg gaaaacttaa ccctatagaa gtaatagccg
      781 atggagattg gatttcttac aaacaagctc aagatacact tacagtaatc aggaattttt
      841 taaatagttt tgactggaga aatgcatctg agatggaaag agctaataga gctgcaaaat
      901 tggtaaccga agcaaaatat gcagactcta aatattgtaa catagtctat ggtaatttgg
      961 ttgataaacg tggtgtatgt ggaagttttg caagtagctt tcatttatta acaaggttaa
     1021 tgggaatgga ttcactatct atattaaatc cgtcattaaa tcatgcttgg aactatatac
     1081 aaattgatgg gaaatggtat aggtttgatg ggtctgaaat atcagctttt ggtggatcat
     1141 tagactttga ttaccgtaaa ttaaaggatg caacgagaga gaagactact tactatgatg
     1201 ctaaggcatt gtctatatta ggatttaatc aataaaaata gggaaggctt cttatggaga
     1261 ctttttttat tgatttgaaa tttaataagg aattttatat gaaaatttaa tcaataaaag
     1321 caaaaaaggc tctttataaa gcctttttaa gtgttttttt aagtgaaatt ttttatataa
     1381 cattagtttt aatcaaaaaa tataacattt tggacttact tttaaaaata aaattattaa
     1441 aatataatat taatcatgt
//
LOCUS       DAEPXF010000355         1366 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain HB27
            SAMN13376389-rid12479423.denovo.363, whole genome shotgun sequence.
ACCESSION   DAEPXF010000355 DAEPXF010000000
VERSION     DAEPXF010000355.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13376389
            Sequence Read Archive: SRR10518432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1366)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1366)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001069624.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001069624.1
            Genome Coverage       :: 315x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/15/2021 01:54:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,165
            CDSs (total)                      :: 4,076
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 89
            rRNAs                             :: 10, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 10 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 19 of 69
            Pseudo Genes (incomplete)         :: 44 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 10 of 69
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1366
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13376389-rid12479423.denovo.363"
                     /strain="HB27"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="China: Hebei"
                     /collection_date="2017"
     gene            210..1307
                     /gene="ychF"
                     /locus_tag="KRL67_003910"
     CDS             210..1307
                     /gene="ychF"
                     /locus_tag="KRL67_003910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HBH1524712.1"
                     /translation="MKLGIVGLPNVGKSTLFNAITQAGAESANYPFCTIDPNVGVVSV
                     PDERLNKLQELYNSEKIVPTAIEFCDIAGLVRGASKGEGLGNKFLSHIREVDAIVHVV
                     RCFEDENVVHVDGSVDPLRDIETINLELIFSDIEILERRINKTQKAAKADKTLGSELD
                     LLKSIMSTLEESKCVRTMEFTEDEQTFVNSLDLLTSKPVIYASNVSEDDLADNGENNK
                     YVQQVKAFAETEDAEVVVVCAQIEAEISELDSAEEKKEFLETLGLEQSGLDKLIKSSY
                     ALLGLISFLTAGPKEVRAWTIKVGSKAPQAGGKIHSDIERGFIRAETIAFNDLVEHGT
                     MAAAKEKGLVKLEGKEYIVKDGDVILFRFNV"
BASE COUNT          530 a          150 c          244 g          442 t
ORIGIN      
        1 ttattatttt ctaaaagcaa tatttttatt taaattaaat acaaatatta tttaaatcaa
       61 tatgctttaa agtctagttt acaagcttga tatatatgat gtataatcaa tatgtatgtc
      121 tataattaaa acaattttgc aatcctatag attgattgtt taaagttata taaagaatat
      181 aaaaaagaat aagagagagg acgattaata tgaaattagg tatagttggt ttaccaaatg
      241 taggtaaaag cacactattt aacgcaataa ctcaagcagg tgcagaatct gcaaattacc
      301 ctttttgtac aatagaccca aatgtgggag ttgtatctgt tccagatgaa agattaaaca
      361 aactacaaga attatataat tctgaaaaaa tagttcctac tgctatagaa ttttgtgata
      421 tagcaggttt agttagaggt gcttctaaag gagaaggctt aggaaataaa ttcttatcac
      481 atataagaga agttgatgca atagttcatg ttgttagatg ttttgaggat gaaaatgttg
      541 tacatgtaga tggttctgta gacccacttc gcgatataga aactataaac ttagaattaa
      601 ttttctcaga tatagagatt cttgaaagaa gaattaataa aactcaaaaa gctgctaagg
      661 ctgataaaac tttaggttct gaacttgatt tattaaaatc aataatgtct actttagaag
      721 aaagtaagtg tgtaagaact atggaattta cagaagatga acaaacattc gtaaattcgt
      781 tggatttact tacatctaag cctgtaatat atgcttcaaa tgtatcagaa gatgatttag
      841 ctgataatgg agaaaataat aaatatgttc aacaagttaa agcatttgca gaaacagaag
      901 atgccgaagt tgttgtagta tgtgcacaaa tagaagcaga aatttctgaa cttgattctg
      961 ctgaagaaaa gaaagagttt ttagaaacat taggtcttga acaatcaggg cttgataagc
     1021 ttataaaatc ttcttatgct ttacttggtt taatttcatt cttaactgct ggtccaaaag
     1081 aagttagagc atggactata aaagttggaa gtaaagctcc tcaggcagga ggaaaaatac
     1141 attctgatat agaaagagga tttatcagag cagaaacaat agcctttaat gatttagttg
     1201 agcatggtac tatggctgct gctaaagaaa aagggttggt taaattagaa ggtaaagaat
     1261 acatagttaa agatggagat gttatattat ttagatttaa tgtttaaaat gattaaacat
     1321 atacacctta actattgtat gtatgataat tagaaaatta aactta
//
LOCUS       DAEPXF010000356         1364 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain HB27
            SAMN13376389-rid12479423.denovo.364, whole genome shotgun sequence.
ACCESSION   DAEPXF010000356 DAEPXF010000000
VERSION     DAEPXF010000356.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13376389
            Sequence Read Archive: SRR10518432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1364)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1364)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001069624.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001069624.1
            Genome Coverage       :: 315x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/15/2021 01:54:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,165
            CDSs (total)                      :: 4,076
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 89
            rRNAs                             :: 10, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 10 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 19 of 69
            Pseudo Genes (incomplete)         :: 44 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 10 of 69
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1364
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13376389-rid12479423.denovo.364"
                     /strain="HB27"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="China: Hebei"
                     /collection_date="2017"
     gene            5..286
                     /locus_tag="KRL67_003911"
     CDS             5..286
                     /locus_tag="KRL67_003911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH1524713.1"
                     /translation="MASKQTPRNNLEKFLRIRVLSHTGDKIHIKLPVNFAKRMIENNA
                     LDLFNGKDDVVDGKKVTEILVKAFDYDLTGEVVHLERKNGDIIKVSIVQ"
     gene            550..804
                     /locus_tag="KRL67_003912"
     CDS             550..804
                     /locus_tag="KRL67_003912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH1524714.1"
                     /translation="MENKDFKLTICIFAFVIGVVVLFCCIGLGEFSAEHIIQQNGGIM
                     DTDQYNIYLDQSISQYRNFGSILALLGGIGILFDKYIRKY"
     gene            849..1172
                     /locus_tag="KRL67_003913"
     CDS             849..1172
                     /locus_tag="KRL67_003913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH1524715.1"
                     /translation="MATEIFTVKITPLEAQKMIKENLDADLVFSDSYNLGDDKFILIT
                     TFEKYYARNNSDAGLIVVYENTTGKTVIKATSTGSAIGILKIDWGAGGNLLKRVKNIL
                     ADYVI"
BASE COUNT          564 a          109 c          221 g          470 t
ORIGIN      
        1 tcgtatggca tcaaaacaaa caccaagaaa caatttagaa aaatttttaa gaataagagt
       61 attatcacat acaggcgata aaatacatat aaagttacct gtaaattttg caaagagaat
      121 gattgagaat aatgctttgg atttatttaa tggaaaagat gacgtagtag atggtaaaaa
      181 ggttactgaa atattagtta aagcatttga ctatgattta actggagaag tagtccactt
      241 agagagaaaa aatggagaca ttattaaagt ttcaattgta caatagaatt attttatatg
      301 aattttattt ttgaatagtt ataaataatt agaaatttgt aaaaaagtat aatatattat
      361 tagtaatggt tcagaataca catttataat ttatagatgt gtattttttg tttaaaaaat
      421 ttaaatatat aaaactataa aggttacaat tttggaaata gatgtaaata tatgttctat
      481 tgtataataa gagtaatata aaatctagta aaaataatca aataaattta gcttatgaga
      541 gggataacta tggaaaataa ggattttaaa ttaacaatat gtatatttgc ttttgtaatt
      601 ggagtagtag tattattctg ctgtataggt ttaggtgaat ttagtgcaga gcatattatt
      661 caacaaaatg gtggaatcat ggatactgac caatataata tttatctaga ccaatctatt
      721 tctcaatata gaaattttgg ttctatatta gctctgttag gaggaatagg gatattgttt
      781 gataaatata tcagaaaata ctagagatta tgttttgtct aaaataaatt cttatgagga
      841 gataaaaaat ggcaacagaa atatttactg taaaaattac acccttagaa gcacagaaaa
      901 tgataaaaga aaatcttgat gcagatttag tttttagtga ttcttataat ttaggagatg
      961 ataaatttat actaattaca acttttgaga agtattatgc aagaaataat agtgatgcag
     1021 gattaatagt agtttatgaa aataccacag gaaaaacagt tattaaagct acttcaacag
     1081 gcagtgcaat tggaatttta aaaattgatt ggggtgcagg tggaaactta cttaaaagag
     1141 taaaaaacat acttgctgat tatgttattt aattatttta tctaactgat aatatgtcaa
     1201 gaaaatctcg taaaagaggt taagaacatt tttataatat atagataaat aagaatttga
     1261 aggtaaagtt tttaaggtat aaagaaaaat aatataataa aacgaattgg ctttataatt
     1321 ctttataagg caaagagcac atttctttat ataattgttg gata
//
LOCUS       DAEPXF010000357         1363 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain HB27
            SAMN13376389-rid12479423.denovo.365, whole genome shotgun sequence.
ACCESSION   DAEPXF010000357 DAEPXF010000000
VERSION     DAEPXF010000357.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13376389
            Sequence Read Archive: SRR10518432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1363)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1363)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001069624.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001069624.1
            Genome Coverage       :: 315x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/15/2021 01:54:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,165
            CDSs (total)                      :: 4,076
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 89
            rRNAs                             :: 10, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 10 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 19 of 69
            Pseudo Genes (incomplete)         :: 44 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 10 of 69
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1363
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13376389-rid12479423.denovo.365"
                     /strain="HB27"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="China: Hebei"
                     /collection_date="2017"
     gene            34..1314
                     /locus_tag="KRL67_003914"
     CDS             34..1314
                     /locus_tag="KRL67_003914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HBH1524716.1"
                     /translation="MNILGLIVEYNPFHNGHIYHLSKSIEKTNATHTVAIMSGNFLQR
                     GEPALFDKYTRAKIAVENGVDLVIELPTLFACQSAEIFSHGAITILNSLNCINSICFG
                     SEEGNTEILYNIAEILANEPNEFKLLLKKYLDDGLLFPTARTNALFDYIHQNKKNNFI
                     QNYSKEQLSKTLNSSNNILGIEYIKNLIHLNSSIKPFTISRIQSEYNSEEIESDICSA
                     TAIRTSLKENTDLFHLKNVIPEKTYNILNEKIDNGFLPMFDDFFFDILKTIILRDMDT
                     LKEYFEVNEGIENRIYKDIFTSNSLHELQLSIKSKRYTLTKIKRTLNNILLGIKKSDM
                     QLIKNIDTIPYIRILAFNDKGREILKEIKNKSEIKIINKFSNISFSLDDTIFKTLISY
                     DIKATNIYNSIYYKNNKDLVKGPMDFYTHPIYVK"
BASE COUNT          586 a          176 c          155 g          446 t
ORIGIN      
        1 atataaaata actaaagata aaaggagtat tttatgaaca tacttggatt aatagttgaa
       61 tataacccat ttcataatgg acacatatat catttatcta aatctataga aaagacaaat
      121 gcaacacata ctgtagcaat aatgagtgga aattttctac aaagaggcga accagcttta
      181 tttgataaat acactagagc aaaaattgct gtcgaaaatg gtgtagactt agttatagaa
      241 ttaccaactt tatttgcttg ccaaagtgct gaaatttttt ctcatggtgc tataacaatt
      301 ttaaattcac taaactgcat aaattctata tgttttggca gtgaagaagg aaatacagaa
      361 attctttata atatagctga aattttagca aatgaaccaa atgaatttaa gttactctta
      421 aaaaagtatt tagatgatgg tctgttgttt ccaacagcta gaactaatgc actttttgat
      481 tatatacatc aaaacaaaaa aaataatttt atacaaaatt attcaaaaga acaattatca
      541 aaaacattaa attcatcaaa taacatactg ggtatagaat atataaaaaa cctcatccat
      601 ttaaacagta gtataaaacc atttactata tcaaggattc aatcagaata taattccgaa
      661 gaaattgaga gtgatatttg ttctgctact gctataagaa cttcacttaa agaaaataca
      721 gacctttttc atttaaaaaa tgttattcca gaaaaaactt ataacatcct aaatgaaaaa
      781 atagataatg gttttttacc tatgtttgat gacttcttct ttgatatttt aaaaacaatt
      841 atacttagag atatggacac tttaaaagag tattttgaag taaatgaagg catagaaaac
      901 agaatctata aagatatctt tacatctaac tctctacatg aactacaatt atcaattaag
      961 tctaagcgat acactttaac caaaatcaaa cgaactctaa acaatatatt gttaggtatt
     1021 aaaaaaagcg acatgcaact tataaaaaac atagatacta taccttatat aagaatactt
     1081 gcttttaatg ataaaggtag agaaattcta aaagagataa aaaataaatc tgaaataaaa
     1141 ataataaaca aattttcaaa tatatcattt tcattagatg atacgatttt taaaacactc
     1201 ataagttatg atataaaagc tacaaatata tacaattcaa tatattataa aaacaataaa
     1261 gatttagtaa aaggccctat ggatttttat actcatccaa tatatgtaaa gtagtttttt
     1321 taaataacta tatttataaa aaccatttta ttaactcttt att
//
LOCUS       DAEPXF010000358         1352 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain HB27
            SAMN13376389-rid12479423.denovo.366, whole genome shotgun sequence.
ACCESSION   DAEPXF010000358 DAEPXF010000000
VERSION     DAEPXF010000358.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13376389
            Sequence Read Archive: SRR10518432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1352)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1352)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001069624.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001069624.1
            Genome Coverage       :: 315x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/15/2021 01:54:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,165
            CDSs (total)                      :: 4,076
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 89
            rRNAs                             :: 10, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 10 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 19 of 69
            Pseudo Genes (incomplete)         :: 44 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 10 of 69
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1352
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13376389-rid12479423.denovo.366"
                     /strain="HB27"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="China: Hebei"
                     /collection_date="2017"
     gene            complement(406..1245)
                     /locus_tag="KRL67_003915"
     CDS             complement(406..1245)
                     /locus_tag="KRL67_003915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HBH1524717.1"
                     /translation="MFGIDKKLIEYIIAKSNYSELSSKSAEVSFFLMLSIFPFLIFTI
                     SSIAYIPILHLNKYIALFRNMMPEGAFAVLSSIIVSAIDNRNLKFLAVSFVLTMWTFS
                     RAVKALIKGMNRAYKVKETRSFLKILSISFLFTIMLLVLIFLSMIFLVYGEKIGYFIF
                     NLIGLDEIFIKIWDILRYTVGIITIIVIFTLLYKYTPNKKLTIKESAPGAIFATFAWF
                     LVSFLYSYYTNYYANYEVIYGSIAEIIVLMTWMYFSSWSIVIGYEVNSRLYFRKIRHE
                     MLK"
BASE COUNT          533 a          192 c          127 g          500 t
ORIGIN      
        1 attttatgta atttattatt tgtttataat tatttatgtc gcttttttct aaatctaatt
       61 acagtagctt attctaatat attggtaatt aggaacacag gcgcatataa aaacacatga
      121 ctaaatactg cattatttga aaatacagac attaactcat taggactata atttttattt
      181 agtatatttt ttgatgcttc taccaatttt atctcttact tatcaatagt catcttttca
      241 aagatgatta taaattaaaa ttatctcata aaataaagtt tgtcaagtta tactaaaaat
      301 aaaaaaagcc cagaaaaatt ctagactttt taacttaaat gtcaatattt ctttattaat
      361 tcaattacat ttataatatt aaatcctatc ttttatttta atactttact ttaacatttc
      421 atgtcttatc tttctaaaat acaatcttga atttacctca tagccaataa cgatagacca
      481 actactaaaa tacatccagg tcataagtac aattatttcg gctatgcttc cgtagattac
      541 ttcatagtta gcatagtaat ttgtataata agaatataaa aacgatacta aaaaccaagc
      601 aaaagtagca aagatggcac ctggcgcaga ttctttaata gtcaactttt tatttggagt
      661 atatttatat aaaagtgtaa atatcactat tattgttatt atacctacag tgtatcttaa
      721 aatatcccat atttttataa atatttcatc caatcctata aggttaaaaa taaaataacc
      781 tattttttct ccataaacta gaaatatcat agataaaaaa ataagtacca aaagcattat
      841 tgtaaataaa aatgatatag ataaaatttt taaaaaagac ctagtttctt taactttata
      901 agctctattc attcctttaa taagagcttt tactgctctt gaaaatgtcc acatagtaag
      961 tacaaaactg actgctaaaa atttcaggtt tctattgtct attgcagaaa ctataattga
     1021 agataacaca gcaaaagcac cttctggcat catatttcta aacaaagcta tatacttatt
     1081 taaatgaaga attggtatat aagctataga acttattgta aatatcaaaa aaggaaatat
     1141 agacaacatt aagaagaaag atacttctgc tgactttgaa cttaattcac tgtaattgga
     1201 ttttgcaatt atatattcaa tcaatttctt atctatacca aacataaata tccccttttc
     1261 tatcaaatta atttcattat acaatattaa ttatactcag attcattcta taatataatt
     1321 atttaagtaa aaataacaca aataaaagct ga
//
LOCUS       DAEPXF010000359         1338 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain HB27
            SAMN13376389-rid12479423.denovo.367, whole genome shotgun sequence.
ACCESSION   DAEPXF010000359 DAEPXF010000000
VERSION     DAEPXF010000359.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13376389
            Sequence Read Archive: SRR10518432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1338)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1338)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001069624.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001069624.1
            Genome Coverage       :: 315x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/15/2021 01:54:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,165
            CDSs (total)                      :: 4,076
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 89
            rRNAs                             :: 10, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 10 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 19 of 69
            Pseudo Genes (incomplete)         :: 44 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 10 of 69
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1338
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13376389-rid12479423.denovo.367"
                     /strain="HB27"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="China: Hebei"
                     /collection_date="2017"
     gene            <1..>1338
                     /locus_tag="KRL67_003916"
     rRNA            <1..>1338
                     /locus_tag="KRL67_003916"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          357 a          292 c          397 g          292 t
ORIGIN      
        1 gctgggatgt gatggggagc gaaatttagt agcgaagtag ctgatttcac actgtcaaga
       61 aaagtctcta tcgaggttaa aggtacccgt accgcaaacc gacacaggtg ggtgaggaga
      121 gtatcctaag gccagcgaga gaactgttgt taaggaactc ggcaaaatga ccccgtaact
      181 tagggataag gggtgccacc atccggtggc cgcagagaat aggcccaagc gactgtttac
      241 caaaaacata ggtttctgct aagtcgcaag acgatgtata ggagctgacg cctgcccggt
      301 gctggaaggt taaggggatc tgttagagca atcgaagcag tgaacttaag ccccagtaaa
      361 cggcggccgt aactataacg gtcctaaggt agcgaaattc cttgtcgggt aagttccgac
      421 ccgcacgaaa ggcgtaacga tttgggcact gtctcaacaa cagactcggt gaaattgtaa
      481 ttccggtgaa gatgccggat acctgcgaca ggacggaaag accccatgga gctttactgt
      541 agcttgacat tgggtcttgg tactacatgt acaggatagg tgggaggctt tgaaaccagg
      601 acgccagttt tggcggagcc atccttggga taccaccctt gtagtactgg gactctaacc
      661 ataggccatg aatctggtct tgggacactg tcaggtgggc agtttgactg gggcggtcgc
      721 ctcccaaaag gtaacggagg cgctcaaagg ttctctcagt acggtcggaa atcgtacgta
      781 gagtgtaaag gcaaaagaga gcttgattgc aagacataca ggtcgagcaa ggatgaaaat
      841 cggacttagt gatccggtgg ttctgcgtgg aagggccatc gctcaacgga taaaagctac
      901 cctggggata acaggcttat ctcccccaag agtccacatc gacggggagg tttggcacct
      961 cgatgtcggc tcatcacatc ctggggctgt agtaggtccc aagggttggg ctgttcgccc
     1021 attaaagtgg tacgcgagct gggttcagaa cgtcgtgaga cagttcggtc cctatccgtc
     1081 gcaggcgtag gaaatttgag aagacctgtc cttagtacga gaggaccggg atggacgtac
     1141 ctctggtgta ccagttgttc tgccaagggc atggctgggt agctatgtac ggaatggata
     1201 agcgctgaaa gcatctaagc gcgaagccaa cttcaagata agatttccca ccgcaagggt
     1261 aagaccccag aaagactatc tggttgatag gtcgaaggtg taagtgcagc aatgtattta
     1321 gcttatcgat actaatag
//
LOCUS       DAEPXF010000360         1328 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain HB27
            SAMN13376389-rid12479423.denovo.368, whole genome shotgun sequence.
ACCESSION   DAEPXF010000360 DAEPXF010000000
VERSION     DAEPXF010000360.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13376389
            Sequence Read Archive: SRR10518432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1328)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1328)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001069624.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001069624.1
            Genome Coverage       :: 315x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/15/2021 01:54:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,165
            CDSs (total)                      :: 4,076
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 89
            rRNAs                             :: 10, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 10 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 19 of 69
            Pseudo Genes (incomplete)         :: 44 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 10 of 69
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1328
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13376389-rid12479423.denovo.368"
                     /strain="HB27"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="China: Hebei"
                     /collection_date="2017"
     gene            complement(96..1184)
                     /locus_tag="KRL67_003917"
     CDS             complement(96..1184)
                     /locus_tag="KRL67_003917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH1524718.1"
                     /translation="MSDKVNMKNIICIAGAYLATAIGSGFATGQEILQFFASQGYMGL
                     IGALISMVLFSFLGAEVITRGRELKLKEPTRIYTFYCGKILGKFYEWFSPIFLFGILI
                     VMISGAGSTLTEYYGINPYLGRVLMAVATLITVSMGLEKLVSILGRIGPVIAVFTIAV
                     GLISLFKHLDGLSMVGEAMKNIEVTKASESPIVSGVIYNTLNIIVMIVFLTGMGASIE
                     SKKDAFWGGLLGGIVFMTAGIVMYLAMISDIGNLYMKEIPSLYLADNIAPIVGVCFSV
                     ALILGIYTTAVPLLWSVTNRIVEDEHPKFKLVTTVIAILACIGGFLPFGKLVNILYPY
                     TGYMGLLILVCMVYKAITCREKSTQSQK"
BASE COUNT          479 a          242 c          140 g          467 t
ORIGIN      
        1 tattatcatg ttttgcaatt tatttaacta ttttgacttt ttaaattcgg attatataaa
       61 aaatttcttt aaataaaaac tattaataag tggctctatt tctgactttg tgttgatttt
      121 tctctacatg taattgcttt atataccata cagactaaaa ttaaaagccc catataacct
      181 gtatatggat ataaaatgtt tactagttta ccaaatggta aaaaaccacc tatacaagcc
      241 aaaatagcta ttactgttgt taccaattta aatttaggat gttcatcttc aacaattcta
      301 ttggttacag accacaaaag tggaacagct gttgtataga tacctaatat taatgcaact
      361 gaaaaacata cacctacaat aggagctata ttatctgcta aataaagtga tggtatttct
      421 ttcatgtaca aatttcctat atctgaaatc atagccaaat acataactat accagcagtc
      481 ataaacacta ttccacctaa taaacctccc caaaaagcat cctttttact ttcaatagaa
      541 gctcccattc ctgttaaaaa aacaatcatt acaataatat ttaatgtatt atatattaca
      601 ccagaaacga ttggactttc tgatgctttt gtaacttcaa tatttttcat agcttctcca
      661 accatagata aaccatcaag atgcttaaac aagcttatta atccaacagc aattgtaaat
      721 actgctatca ctggtccaat acgtccaagt atgcttacta atttttctaa acccatagac
      781 actgtaatta aagtagcaac agccatcaac actcttccta agtaaggatt tataccatag
      841 tattcagtta aggttgagcc tgcacctgaa atcattacta taagaatccc aaataaaaat
      901 attggactaa accattcgta aaactttcct aaaatctttc cacaataaaa agtgtaaatt
      961 cttgttggtt ctttaagttt taattctcta cctctagtta taacttcagc accaagaaat
     1021 gaaaacaata ccattgatat tagtgctcca attaaaccca tatatccttg tgaggcaaaa
     1081 aattgtagaa tttcttgacc agtagcaaat ccagaaccaa ttgctgtagc taaataagca
     1141 cctgcaatac agataatatt tttcatattc actttgtctg acatattaaa ttacctccat
     1201 tataaagtta ccttcctcta agaaatataa actctcggta aattaaatga aaattctgtc
     1261 tttttagaat agtatttaat tcactttttt tattttgtat atctttttct ttttatttat
     1321 atatattt
//
LOCUS       DAEPXF010000361         1308 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain HB27
            SAMN13376389-rid12479423.denovo.369, whole genome shotgun sequence.
ACCESSION   DAEPXF010000361 DAEPXF010000000
VERSION     DAEPXF010000361.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13376389
            Sequence Read Archive: SRR10518432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1308)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1308)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001069624.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001069624.1
            Genome Coverage       :: 315x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/15/2021 01:54:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,165
            CDSs (total)                      :: 4,076
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 89
            rRNAs                             :: 10, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 10 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 19 of 69
            Pseudo Genes (incomplete)         :: 44 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 10 of 69
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1308
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13376389-rid12479423.denovo.369"
                     /strain="HB27"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="China: Hebei"
                     /collection_date="2017"
     gene            complement(241..1122)
                     /locus_tag="KRL67_003918"
     CDS             complement(241..1122)
                     /locus_tag="KRL67_003918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline iminopeptidase-family hydrolase"
                     /protein_id="HBH1524719.1"
                     /translation="MKITEGYMPFKGFKTYYRIVGENTEGKKPLVLLHGGPGSTHNYF
                     EVLDKIAESGRQVIMYDQIGCGNSFVEGHPELFNADTWIEELIELRKHLGLDEIHLLG
                     QSWGGMQAIWYAIEYKPKGIKSYILSSTLSSAKLWEKEQKRRISYMSEADQKALLDAV
                     NTGDYSSKEYNDALERFMEMYCAGEVTEDSPECLRRPKKSGSEAYIVGWGQNEFSPTG
                     TLSGYEFTDRLHEIKEPCLVTSGAIDLCSPYIAKTMYDRIPNSKWELFEYSRHMPFVE
                     ENEKYIKVLTEWLDAND"
BASE COUNT          436 a          239 c          142 g          491 t
ORIGIN      
        1 tttctaaaaa gcaataaaat taagttataa gcactaatat atttctatta ttttaataag
       61 tatacaatta gattagaatc taacgctagt aataaaaaaa ttactttccc aaaatagata
      121 taagcctcat tttatcgttc aaatgatgaa aatatatttg atttcatgaa aaaagagtgc
      181 ctaatgaact aaaaagttca ttttgagaca ctcatttttt tatataaatt tatatagttt
      241 ttaatcgttc gcatctaacc actcagttaa gacttttata tatttctcat tttcttcaac
      301 gaatggcata tgtcttgaat actcaaataa ctcccactta ctattaggta ttctatcata
      361 catagtcttt gcaatatatg gtgaacataa atctattgct ccactagtta ctagacaagg
      421 ctcttttatt tcatgtaatc tatctgtaaa ttcatatcca gaaagagttc ctgtaggaga
      481 aaattcattt tgtccccatc ctacaatata tgcttcagaa cctgattttt taggtcttct
      541 caggcactct ggagaatctt ctgtcacttc acctgcacaa tacatttcca taaatctttc
      601 taatgcatca ttatattctt tgctagagta atctccagta tttactgcat caagtaatgc
      661 tttttggtca gcctcactca tatatgatat tcttcttttt tgttctttct cccaaagttt
      721 tgcagaagaa agagtagatg aaaggatgta agacttaata ccttttggtt tatattcaat
      781 agcataccaa atagcctgca tacctcccca agattgacct aacagatgaa tttcatcaag
      841 tcctagatgt tttcttagtt caataagctc ctctatccaa gtatctgcat taaataactc
      901 aggatgccct tctacaaatg aatttccaca accaatttgg tcatacatta ttacttgtct
      961 accactttca gctattttat ccaatacttc aaaatagttg tgtgtagaac ctggacctcc
     1021 gtgaagtaat actaatggct tcttcccttc tgtattttct cctactatac ggtaatacgt
     1081 tttaaaacct ttaaaaggca tatatccttc agtaattttc atgtttatct cctccaattt
     1141 gtaattataa acaataatca agttcattaa caatgctatt taaaacttct tatacatatt
     1201 tgattatgta ttttaatctt tatacctact tttataaata gtataacata aaagttaaaa
     1261 atctctatac taaaccaatt ataaaatcat attaactata taatttta
//
LOCUS       DAEPXF010000362         1305 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain HB27
            SAMN13376389-rid12479423.denovo.370, whole genome shotgun sequence.
ACCESSION   DAEPXF010000362 DAEPXF010000000
VERSION     DAEPXF010000362.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13376389
            Sequence Read Archive: SRR10518432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1305)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1305)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001069624.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001069624.1
            Genome Coverage       :: 315x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/15/2021 01:54:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,165
            CDSs (total)                      :: 4,076
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 89
            rRNAs                             :: 10, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 10 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 19 of 69
            Pseudo Genes (incomplete)         :: 44 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 10 of 69
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1305
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13376389-rid12479423.denovo.370"
                     /strain="HB27"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="China: Hebei"
                     /collection_date="2017"
     gene            398..1105
                     /locus_tag="KRL67_003919"
     CDS             398..1105
                     /locus_tag="KRL67_003919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HBH1524720.1"
                     /translation="MIFTKSNKYDKDFLMENMMGPNCIKILEELTSKIKLEKGMRILD
                     LGCGKGISSIFLAKEFDATVFATDLWIEPTENYKRFKEFKLDDKIFPIQAEAHELPYA
                     EGFFDAVISVDSYHYFGNKEGFLDNHISPLVKEGGILAMAMPGLKEDFVDGIPDELIP
                     FWQDNMNFHSITWWNKLWSESESVIVEKCEALNCHDEAWKDWINCDNSYAINDKKMME
                     VENGKYFNTISLIARTK"
BASE COUNT          538 a          122 c          231 g          414 t
ORIGIN      
        1 caaatatgca ctaaattaaa taagttaatt agtatatagc ttaaagtgga agtaattaaa
       61 acttattaag tatcagaaaa cgacttctta ttagagtaag tatctaaaat gatataaaaa
      121 ttatgattac tcattaaagt gactattaag tagataaaat tacccattaa aataactatc
      181 atagagtgga taaaattact cattaaagtg attattgtag agtagataaa attactcatt
      241 aaaataacta tcatagagtg gatgagatta ctcattaaaa ttgcctgtta aagaatgaat
      301 aaaaaagaga ttgctcatta taagaagcat ataaaaaata aaaaatcaaa cgtataaaat
      361 ttaataaata ataacggata aggaaaggaa agatagaatg atatttacaa aaagtaataa
      421 atacgataag gactttctca tggaaaatat gatgggacct aattgtataa aaatattgga
      481 agaattaaca agtaagataa aactagaaaa gggaatgcgt attttagact taggttgtgg
      541 taagggtata tcctcgatat ttttagctaa ggaatttgat gctactgtat ttgcaacaga
      601 tttatggata gaaccaactg aaaactataa aagatttaaa gagtttaaat tggatgataa
      661 aatatttcca atacaggcag aggcacatga actaccttat gcagaaggtt tctttgatgc
      721 ggtgataagt gtcgattctt atcactattt tggaaacaaa gaaggatttt tagacaatca
      781 tatatcacct cttgtaaaag aggggggaat acttgctatg gcaatgcctg gactaaaaga
      841 agattttgtg gatggtatac cagatgaact tattccattt tggcaagata atatgaactt
      901 ccattcaatt acatggtgga ataaactttg gtctgaatct gagtctgtga ttgtagagaa
      961 atgtgaagct ttaaattgtc atgatgaagc ttggaaggat tggataaatt gcgataattc
     1021 atacgctata aatgacaaaa aaatgatgga agtagagaat ggaaagtatt ttaatacaat
     1081 ttctttgata gcaagaacta aataattgtg atgaatttat aaaagtataa gaagcataga
     1141 aatataagtt gaattaaagt agaagttaaa taaaaatgta agttaaatga agagtttata
     1201 caaaaatgat tttttagatt atttataccc aatcgagttg tttaagtcag ttgggtattt
     1261 ttatttttta aagttaaatt gcaaatacta ttgtgtgata tatat
//
LOCUS       DAEPXF010000363         1294 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain HB27
            SAMN13376389-rid12479423.denovo.371, whole genome shotgun sequence.
ACCESSION   DAEPXF010000363 DAEPXF010000000
VERSION     DAEPXF010000363.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13376389
            Sequence Read Archive: SRR10518432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1294)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1294)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001069624.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001069624.1
            Genome Coverage       :: 315x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/15/2021 01:54:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,165
            CDSs (total)                      :: 4,076
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 89
            rRNAs                             :: 10, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 10 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 19 of 69
            Pseudo Genes (incomplete)         :: 44 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 10 of 69
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1294
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13376389-rid12479423.denovo.371"
                     /strain="HB27"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="China: Hebei"
                     /collection_date="2017"
     gene            <1..>1294
                     /locus_tag="KRL67_003920"
     CDS             <1..>1294
                     /locus_tag="KRL67_003920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH1524721.1"
                     /translation="SLIIKTLYEKELVCDTKDQTYIQIIIAILRNIQNNGFYRVISKK
                     SKLDINIWRKFVQQSIKYDQIIYDLPKYHYDLLNDELLNIKHGYINMPTNYSKIEVIK
                     IVIFNYLHTYQDKKIAYIHVDKDYRAINAIKEYFTEYSTRIEFIEIDSINNYSEIATD
                     NNFIIIDDFSNVSNKYYGTRFEIMISLALKLEKRLLVLNNNYLKMDSNQLENINYNED
                     INRYSNIVNYYFKDGKNKELEIEYEKERFVTESYLLNKEINNKINLKGIKLNSAGDKS
                     LLSAINLISKGYKVSIFIPQKNSAKSGINKICIKLLNLIDRLNGKILYDKNCIDDLNL
                     LDRAELLLGRESIYMKLFRLGVIVIHSDMPQEVIEYMRYFIQCGKYKIIIGDKILTKN
                     LSKYTDVLIINSIRINIDKSWFIIDDRLLLDLINDFSDQ"
BASE COUNT          585 a           98 c          179 g          432 t
ORIGIN      
        1 agtttaataa taaaaacttt gtatgaaaaa gagcttgtat gtgatactaa agatcagacc
       61 tatatacaaa ttattattgc tatattaaga aatatacaaa ataatggatt ctatagagtt
      121 atttcaaaaa aatcaaaatt agatataaat atatggagaa aatttgtgca acaaagtata
      181 aagtatgatc aaatcatata tgatcttccc aaatatcatt atgatttatt aaatgatgaa
      241 ttattgaata taaagcatgg atatataaat atgcccacta attactctaa gatagaagta
      301 attaagatag taatatttaa ttatttacat acatatcaag ataaaaaaat agcatatata
      361 catgtagata aagattatag ggctataaat gcaattaaag aatattttac tgaatattca
      421 acaagaattg aatttattga aatagattca attaataatt atagtgagat agctactgat
      481 aacaatttta taatcataga tgattttagt aatgtttcaa acaaatacta tggtacacga
      541 tttgaaataa tgatatcatt agcattaaaa ttagaaaaaa ggctattagt attgaataat
      601 aattatctta aaatggactc taatcaactt gaaaatataa attataatga agatattaat
      661 agatatagca atatagttaa ttactatttt aaagatggaa aaaataaaga gcttgaaata
      721 gaatatgaaa aagagagatt tgttacagaa agttatttgt taaataagga gataaataac
      781 aaaataaact taaagggtat taaattgaac agtgcaggag ataaaagttt actatctgca
      841 ataaatttaa tatctaaagg atacaaagta tcaatattta taccacagaa aaattctgca
      901 aaaagtggta taaataaaat atgtataaag ctattaaatt taattgatag gcttaacggt
      961 aaaatattgt acgataaaaa ttgtatagat gatttaaact tattagatag agcagagtta
     1021 ttgttaggta gagaaagtat atatatgaaa ttatttagat taggggtaat tgtaattcat
     1081 tctgatatgc ctcaagaagt aattgaatat atgagatatt ttatacaatg tggaaaatat
     1141 aaaattatca taggagacaa gattttaact aaaaacttaa gtaaatacac agatgtgtta
     1201 attataaata gtattagaat aaatatagat aaatcatggt ttattataga tgatagatta
     1261 ctattagatt taattaacga tttttcagat caga
//
LOCUS       DAEPXF010000364         1292 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain HB27
            SAMN13376389-rid12479423.denovo.372, whole genome shotgun sequence.
ACCESSION   DAEPXF010000364 DAEPXF010000000
VERSION     DAEPXF010000364.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13376389
            Sequence Read Archive: SRR10518432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1292)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1292)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001069624.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001069624.1
            Genome Coverage       :: 315x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/15/2021 01:54:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,165
            CDSs (total)                      :: 4,076
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 89
            rRNAs                             :: 10, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 10 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 19 of 69
            Pseudo Genes (incomplete)         :: 44 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 10 of 69
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1292
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13376389-rid12479423.denovo.372"
                     /strain="HB27"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="China: Hebei"
                     /collection_date="2017"
     gene            complement(151..417)
                     /locus_tag="KRL67_003921"
     CDS             complement(151..417)
                     /locus_tag="KRL67_003921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3795 domain-containing protein"
                     /protein_id="HBH1524722.1"
                     /translation="MIESRCGILCYECGYKEKVNCKGCTEIEKPFWGEQCPVKSCCED
                     KNLTHCGLCDTFPCEMLNQFAYDKEQGDDGKRISQCKKWAVLAL"
     gene            complement(459..1268)
                     /locus_tag="KRL67_003922"
     CDS             complement(459..1268)
                     /locus_tag="KRL67_003922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBH1524723.1"
                     /translation="MNKIINNILNTCKQYTYILPHKLLRQYIAHYTISIPDTSIKENL
                     TLIPDASGCMIFKFDKKGIESAFWGATTKTTIVKNDIENVLFRVFVEFRPGGVYYLTG
                     LSQRESTDLKISLEDFNTLFSLEANSIFERTSTIKELVEQLDMLFLSYLLKSNIVDMT
                     IPILENARKQNSIMSVKNISQISCYSERHLNRIFNNSLGMSVKSYLRLLRINLVLQEV
                     QNNKIPFATLAQDIGYYDQSHFINDFKSICGVNPTTYIKNLSDFYNEKYKF"
BASE COUNT          449 a          193 c          147 g          503 t
ORIGIN      
        1 attttagcaa tcatatttta tcaataaatt actattctat taatttaatt aaataacctt
       61 taaatttaat tataaaaact gttgtagaat tttctacaac agtttaaaaa atttacctta
      121 caaaaactta ttttataaca agagagtata ttataaggct agtacagccc attttttgca
      181 ctgagaaatt cttttgccat catcaccttg ttctttgtca tatgcaaatt gattaagcat
      241 ttcacatgga aatgtatcac atagaccaca atgtgtaagg tttttatctt cacaacaaga
      301 ttttacagga cattgttcac cccaaaatgg tttctcaatc tctgtacaac ctttacaatt
      361 gactttttcc ttgtacccac attcataaca taaaattccg catcttgatt ctatcattct
      421 aagtcactcc ttaaatataa attttatatg attgtatatc aaaatttata cttttcattg
      481 taaaaatcgg acaaattttt tatataggtt gttggattaa caccacaaat agatttaaaa
      541 tcatttataa aatgagattg gtcgtaataa ccaatatctt gtgctaaagt cgcaaatggt
      601 attttattat tttgtacttc ttgtaataca aggtttatac gaagtaatct aaggtatgat
      661 ttaacactca tcccaagaga attattaaaa attctattta aatgtcgttc actataacaa
      721 ctaatttgtg aaatgttttt aacagacata atagaattct gttttctagc attttcaaga
      781 attggtatag tcatatctac tatatttgat ttcaataagt atgataagaa taacatatct
      841 aattgttcta ctaattcctt tatagtactt gttctttcaa aaatggaatt agcctctaag
      901 ctaaataatg tgttaaaatc ttcaagtgaa atttttaaat ctgtagattc tctttgagaa
      961 agacctgtca aataatatac tccacctggg cgaaactcaa cgaatactct aaaaagtaca
     1021 ttttctatat cattttttac aatagtagtt tttgttgtag ctccccaaaa tgcactttct
     1081 ataccttttt tatcaaattt aaatatcata catccactag catcaggtat taatgtaaga
     1141 ttttctttaa tacttgtgtc tggaatagat attgtatagt gtgctatata ctgtcttaac
     1201 aatttatgtg gcaaaatata tgtatactgt ttgcaggtat ttaaaatgtt attgattatt
     1261 ttattcataa gtatatcctc ctgatatata aa
//
LOCUS       DAEPXF010000365         1260 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain HB27
            SAMN13376389-rid12479423.denovo.373, whole genome shotgun sequence.
ACCESSION   DAEPXF010000365 DAEPXF010000000
VERSION     DAEPXF010000365.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13376389
            Sequence Read Archive: SRR10518432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1260)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1260)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001069624.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001069624.1
            Genome Coverage       :: 315x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/15/2021 01:54:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,165
            CDSs (total)                      :: 4,076
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 89
            rRNAs                             :: 10, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 10 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 19 of 69
            Pseudo Genes (incomplete)         :: 44 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 10 of 69
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1260
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13376389-rid12479423.denovo.373"
                     /strain="HB27"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="China: Hebei"
                     /collection_date="2017"
     gene            1..75
                     /locus_tag="KRL67_003923"
     tRNA            1..75
                     /locus_tag="KRL67_003923"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:33..35,aa:Thr,seq:tgt)
     gene            90..174
                     /locus_tag="KRL67_003924"
     tRNA            90..174
                     /locus_tag="KRL67_003924"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:124..126,aa:Tyr,seq:gta)
     gene            185..268
                     /locus_tag="KRL67_003925"
     tRNA            185..268
                     /locus_tag="KRL67_003925"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:219..221,aa:Leu,seq:tag)
     gene            297..373
                     /locus_tag="KRL67_003926"
     tRNA            297..373
                     /locus_tag="KRL67_003926"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:331..333,aa:Arg,seq:tct)
     gene            381..456
                     /locus_tag="KRL67_003927"
     tRNA            381..456
                     /locus_tag="KRL67_003927"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:414..416,aa:Gln,seq:ttg)
     gene            546..634
                     /locus_tag="KRL67_003928"
     tRNA            546..634
                     /locus_tag="KRL67_003928"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:580..582,aa:Ser,seq:tga)
     gene            638..713
                     /locus_tag="KRL67_003929"
     tRNA            638..713
                     /locus_tag="KRL67_003929"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:671..673,aa:Phe,seq:gaa)
     gene            720..793
                     /locus_tag="KRL67_003930"
     tRNA            720..793
                     /locus_tag="KRL67_003930"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:754..756,aa:Met,seq:cat)
     gene            808..884
                     /locus_tag="KRL67_003931"
     tRNA            808..884
                     /locus_tag="KRL67_003931"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:842..844,aa:Ile,seq:cat)
     gene            914..990
                     /locus_tag="KRL67_003932"
     tRNA            914..990
                     /locus_tag="KRL67_003932"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:948..950,aa:Pro,seq:tgg)
     gene            998..1074
                     /locus_tag="KRL67_003933"
     tRNA            998..1074
                     /locus_tag="KRL67_003933"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1032..1034,aa:His,seq:gtg)
     gene            1083..1158
                     /locus_tag="KRL67_003934"
     tRNA            1083..1158
                     /locus_tag="KRL67_003934"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1116..1118,aa:Lys,seq:ttt)
     gene            1166..1239
                     /locus_tag="KRL67_003935"
     tRNA            1166..1239
                     /locus_tag="KRL67_003935"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1198..1200,aa:Cys,seq:gca)
BASE COUNT          309 a          281 c          330 g          340 t
ORIGIN      
        1 gctggtgtgg ctcaacggta gagcagctga cttgtaatca gcaggttgta ggttcgattc
       61 ctatcaccag ctccatgtaa aattaatacg gaggatttcc cgagcggcca aagggggcag
      121 actgtaaatc tgttgtcatc gacttcggtg gttcgaatcc accatcctcc accaaaaaaa
      181 atgcgcggat gtggcggaat tggcagacgc actagactta ggatctagcg ccattggcgt
      241 gggggttcga ctcccttcat ccgcaccatt tttatttgat atacatatat aattgtgggt
      301 gcatagctca gctggataga gcaacgccct tctaaggcgt gtgtccgggg ttcgaatccc
      361 tgtgcgctca ccacatatat tggggattag ccaagtcggt aaggcacatg actttgactc
      421 atgtatgcgt aggttcgagt cctgcatccc cagccaaatg atgtatttac taagctatat
      481 ttgaattatt tttattttaa atatagttag gtttaatttt agaataagtt taagtatccg
      541 aatgtggaga ggtgtccgag tggtttaagg agctggtctt gaaaaccagt gacttcgaaa
      601 ggggccgtgg gttcgaatcc caccctctcc gccaaatgcc cagatagctc agtcggtaga
      661 gcaggggact gaaaatcccc gtgtcggtgg ttcgattccg cctctgggca ccaaaatatg
      721 gcggtatagc ttagttggct agagcgttcg gttcataccc gaaaggtcat aggttcgact
      781 cctattaccg ctactaaaat ttaatgtggg ccattagctc agttggttag agcgcccggc
      841 tcataaccgg taggtctggg gttcgagtcc ctgatggccc accacattaa attaattaat
      901 ataaatataa attcgaggtg tagcgcagtt tggtagcgca catggtttgg gaccatgggg
      961 ccgggggttc gagtcccttc acctcgacca atataaggtg ggtatagctc agttggttag
     1021 agcgccagat tgtggctctg gaggtcgtga gttcgactct cattatccac cccaaattat
     1081 atggtctatt agctcagtcg gtagagcacc tgacttttaa tcagggtgtc ccgcgttcga
     1141 gtcgcggata gatcaccaaa aaggtggcga catagccaag tggtaaggca gtggactgca
     1201 actccttgat ccccagttcg aatctgggtg tcgcctccaa aatattccaa ggtagctcaa
//
LOCUS       DAEPXF010000366         1255 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain HB27
            SAMN13376389-rid12479423.denovo.374, whole genome shotgun sequence.
ACCESSION   DAEPXF010000366 DAEPXF010000000
VERSION     DAEPXF010000366.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13376389
            Sequence Read Archive: SRR10518432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1255)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1255)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001069624.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001069624.1
            Genome Coverage       :: 315x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/15/2021 01:54:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,165
            CDSs (total)                      :: 4,076
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 89
            rRNAs                             :: 10, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 10 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 19 of 69
            Pseudo Genes (incomplete)         :: 44 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 10 of 69
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1255
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13376389-rid12479423.denovo.374"
                     /strain="HB27"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="China: Hebei"
                     /collection_date="2017"
     gene            complement(353..426)
                     /locus_tag="KRL67_003936"
     tRNA            complement(353..426)
                     /locus_tag="KRL67_003936"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(390..392),aa:Arg,seq:tct)
     gene            complement(436..510)
                     /locus_tag="KRL67_003937"
     tRNA            complement(436..510)
                     /locus_tag="KRL67_003937"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(476..478),aa:Gly,seq:gcc)
     gene            complement(521..597)
                     /locus_tag="KRL67_003938"
     tRNA            complement(521..597)
                     /locus_tag="KRL67_003938"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(561..563),aa:Asp,seq:gtc)
     gene            complement(603..678)
                     /locus_tag="KRL67_003939"
     tRNA            complement(603..678)
                     /locus_tag="KRL67_003939"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(643..645),aa:Val,seq:tac)
     gene            complement(683..756)
                     /locus_tag="KRL67_003940"
     tRNA            complement(683..756)
                     /locus_tag="KRL67_003940"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(722..724),aa:Gly,seq:tcc)
     gene            complement(765..839)
                     /locus_tag="KRL67_003941"
     tRNA            complement(765..839)
                     /locus_tag="KRL67_003941"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(804..806),aa:Glu,seq:ttc)
     gene            complement(847..922)
                     /locus_tag="KRL67_003942"
     tRNA            complement(847..922)
                     /locus_tag="KRL67_003942"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(887..889),aa:Met,seq:cat)
     gene            complement(938..1023)
                     /locus_tag="KRL67_003943"
     tRNA            complement(938..1023)
                     /locus_tag="KRL67_003943"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(987..989),aa:Leu,seq:taa)
     gene            complement(1029..1103)
                     /locus_tag="KRL67_003944"
     tRNA            complement(1029..1103)
                     /locus_tag="KRL67_003944"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1069..1071),aa:Asn,seq:gtt)
     gene            complement(1111..1227)
                     /gene="rrf"
                     /locus_tag="KRL67_003945"
     rRNA            complement(1111..1227)
                     /gene="rrf"
                     /locus_tag="KRL67_003945"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
BASE COUNT          343 a          298 c          253 g          361 t
ORIGIN      
        1 ttatattctc taattttttt ataatttctt atccttatcg tttagacaaa aaatatgctc
       61 atgaatctct ttataaataa tttcataaat tatttcaata tataaatacc ataaagtata
      121 caacataatg tatataattc ttatccagtt agttatattt aactggtacc ttcataaaca
      181 tcctgtagac tattatacat tacccttcgt atgtttacaa tattaatgaa tatactgtaa
      241 tttttacata gtttaaagtt tataaacaaa aagacacagg taatatacat gtatcctgtg
      301 tctttttaaa caatataaaa gtttactata aattatagta aacttttctt aatgagcgca
      361 cagggattcg aaccccggac acacgcctta gaagggcgtt gctctatcca gctgagctat
      421 gcacccaaat tattatggag cgggaaacga gattcgaact cgcgacttca accttggcaa
      481 ggttgcgctc taccactgaa ctattcccgc ttatttaatt tggcgacctg gaaggggctc
      541 gaaccctcga cctccagcgt gacaggctgg cattctaacc aactgaacta ccaggccgca
      601 aatggtggga ccaacagggc tcgaacctgt gaccccctgc ttgtaaggca ggtgctctcc
      661 cagctgagct atggtcccat tttggagcgg gtgaagggga tcgaaccctc acagctggct
      721 tggaaggcca gaactctacc attgagctac acccgcatat ttaatggtga ctcgtagggg
      781 aattgaaccc ctgttaccgc cgtgaaaggg cggtgtctta acctcttgac caacgagcca
      841 ttttaatggt tgcggaggca ggacttgaac ctacgacctt cgggttatga gcccgacgag
      901 ctgccaactg ctccaccccg cgatattgtt tcataagtgc cgaagaccgg aatcgaaccg
      961 gtacgagagg taagtcccgc aggattttaa gtcctgtgcg tctgccagtt ccgccacttc
     1021 ggcttttttg gctccaaggg tggggctcga acccacagcc taccggttaa cagccggttg
     1081 ctccaccgtt gagctacctt ggaacattta acgtggctac gtcctactct cccaggcagt
     1141 ttcccaccaa gtaccatcag cgctagagag cttaacttct gtgttcggaa tgggaacagg
     1201 tgtatcctct ctgctattgt aaccacataa tctttatgct taatatttct attaa
//
LOCUS       DAEPXF010000367         1248 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain HB27
            SAMN13376389-rid12479423.denovo.375, whole genome shotgun sequence.
ACCESSION   DAEPXF010000367 DAEPXF010000000
VERSION     DAEPXF010000367.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13376389
            Sequence Read Archive: SRR10518432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1248)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1248)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001069624.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001069624.1
            Genome Coverage       :: 315x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/15/2021 01:54:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,165
            CDSs (total)                      :: 4,076
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 89
            rRNAs                             :: 10, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 10 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 19 of 69
            Pseudo Genes (incomplete)         :: 44 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 10 of 69
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1248
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13376389-rid12479423.denovo.375"
                     /strain="HB27"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="China: Hebei"
                     /collection_date="2017"
     gene            113..1189
                     /locus_tag="KRL67_003946"
     CDS             113..1189
                     /locus_tag="KRL67_003946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--glyoxylate aminotransferase family
                     protein"
                     /protein_id="HBH1524724.1"
                     /translation="MSKKLFIPGPIDVREEVLQKMATPMIGHRGKDASMLQKSISEKM
                     QKLFYTNNTILLSTSSGTGLMEGSIRSCTSKKAAVFSCGSFGDRWYKMAVANNVPADI
                     FKVELGEATMPEMVDKVLSTGEYDLITVTHNETSTGIRNPIEEIGEVVKKYEDVIYCV
                     DTVSSAGGIKVEVDKIGIDICITSVQKALGLPPGMSICTFSQKAIDRAKQVPFRGVYL
                     DLLAMYEYLIKKNYQYPSTPSLSHMFALDFQLDNILDEGLDNRFNRHEDMANLVRNWA
                     KKHFQIFTNENHLSNTLTVIENTQGISVSNLNSKLQERGFQIANGYGDLKEKTFRISH
                     MGDYTVEDVQELLDNIDDILGFNK"
BASE COUNT          489 a          173 c          218 g          368 t
ORIGIN      
        1 taaaaaataa attttatgtt gacatagtta attcagcttg atattatagt tattaaaaat
       61 aaggacagta aaaacgctat ccatattaca aactaaaaag gggggaatta taatgagtaa
      121 gaaattattt attcctggtc ctattgatgt tagagaagaa gtcttgcaaa agatggctac
      181 accaatgata ggacatagag gaaaagatgc atctatgctc caaaagagta taagtgaaaa
      241 gatgcaaaaa ctattctata caaacaacac gatactttta tccacgtcat ccggaactgg
      301 tttaatggaa ggctccattc gttcctgtac atccaaaaag gcagcagtat tttcctgtgg
      361 atcctttggt gatagatggt acaaaatggc agtggctaat aatgtcccag cggatatatt
      421 taaagttgaa ttaggagagg caacaatgcc agaaatggtt gataaggtgc tttctaccgg
      481 agaatacgat ttaataactg taactcacaa tgaaacttca actggaataa gaaatccaat
      541 agaagaaatt ggagaagttg ttaaaaagta tgaagatgta atctactgtg tagatactgt
      601 aagttcagcc ggtggaataa aagtcgaggt tgataagatt ggaatagata tttgtataac
      661 atcagtacaa aaagcactgg gattaccacc aggaatgtct atttgcacat tttctcaaaa
      721 agcaatcgat agagctaagc aagtaccttt tagaggagta tatcttgatt tgttagcaat
      781 gtatgaatac ttaatcaaga aaaactacca atacccttca acaccatctc tttctcacat
      841 gttcgcttta gatttccaat tagataatat attagatgaa ggcctagata acagattcaa
      901 cagacatgaa gacatggcaa acttagttag aaactgggct aaaaaacact tccaaatttt
      961 taccaatgaa aatcacttat ccaatacact tactgttata gaaaatactc aaggtattag
     1021 tgtatcaaat ttaaattcaa aattacaaga acgaggattc caaatagcaa atggctatgg
     1081 agatttaaaa gaaaaaactt ttagaatttc acatatgggt gactatactg tcgaagatgt
     1141 acaagaatta ttagataata ttgatgatat attaggtttt aataaataat attgcaaaaa
     1201 tatgtatgtt ttaagataaa tttagaatat ataataaata atatttta
//
LOCUS       DAEPXF010000368         1236 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain HB27
            SAMN13376389-rid12479423.denovo.376, whole genome shotgun sequence.
ACCESSION   DAEPXF010000368 DAEPXF010000000
VERSION     DAEPXF010000368.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13376389
            Sequence Read Archive: SRR10518432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1236)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1236)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001069624.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001069624.1
            Genome Coverage       :: 315x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/15/2021 01:54:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,165
            CDSs (total)                      :: 4,076
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 89
            rRNAs                             :: 10, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 10 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 19 of 69
            Pseudo Genes (incomplete)         :: 44 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 10 of 69
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1236
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13376389-rid12479423.denovo.376"
                     /strain="HB27"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="China: Hebei"
                     /collection_date="2017"
     gene            106..1044
                     /locus_tag="KRL67_003947"
     CDS             106..1044
                     /locus_tag="KRL67_003947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBH1524725.1"
                     /translation="MKCTNFTFKGEEGLDIYTYKWEDENIKKTKAVIQIAHGMAETAQ
                     RYETFAKVLTENGYIVYINDHRGHGKTAKIIENVGHLAEKEGFRCLVEDMYTLTNIIK
                     KENEDLPIYLFGHSMGSFASQRYIMDYGNNLSGLILCGSNGKQGIILNFAHMIINREI
                     KKYGRRSRSNKINDLIFGGEIIRRNEKTKFDWLSRDKEQVEKYINDPFCGVVCSCGFF
                     YDLVQGLKEIEDKENLKKVPLDIPIYIISGDKDPIGKNGKGVLRLRDRYIKLGVKDVT
                     CKLYKDGRHELLNEINKEEVFEDIICWLNNKIEILR"
BASE COUNT          519 a           98 c          228 g          391 t
ORIGIN      
        1 ggaattaata attcttaaat ttttttagaa agattttgac ttgaaaatac ttgtaatata
       61 atatataaat taatatactg aataaaatca atcggaggga taattatgaa gtgtacaaac
      121 tttactttca aaggagagga aggtttagat atatatacat ataaatggga agatgaaaat
      181 ataaaaaaaa caaaggcagt tatccaaatt gcacatggaa tggctgaaac tgcacaaagg
      241 tatgagactt ttgccaaggt acttactgaa aatggttata tagtatatat caatgatcat
      301 agaggtcatg gaaaaactgc aaagataata gaaaatgttg gacatttagc tgaaaaagaa
      361 ggatttagat gtcttgtaga ggacatgtat actttaacaa atattataaa gaaagaaaat
      421 gaagatttgc caatttatct atttggacat agcatgggtt catttgcaag tcaaagatac
      481 ataatggatt acggtaataa tttatctggt cttatactat gtggttcgaa tggaaaacaa
      541 ggaatcattt taaattttgc tcatatgata atcaatcgtg agattaaaaa gtatggaaga
      601 cgttctagaa gtaataaaat aaatgatttg atatttggtg gcgaaataat aagaagaaat
      661 gaaaagacta agtttgactg gttaagtaga gataaagaac aagttgaaaa atatataaat
      721 gacccatttt gtggagtagt gtgtagttgt ggcttttttt atgatttagt tcaagggtta
      781 aaagaaattg aggataaaga aaacttaaaa aaagtgccac ttgatatacc tatttacata
      841 atatctggag ataaagaccc cataggaaaa aatggtaaag gagtcttaag attaagggat
      901 agatatataa aattaggagt aaaagatgta acttgcaaac tttacaaaga tggaaggcat
      961 gaattgttga atgaaataaa taaggaagaa gtttttgagg acataatttg ttggctaaat
     1021 aataaaatag aaatattgag ataaaagaat ataattatat attaatatta aatttgattc
     1081 tatggaaatg tttgaaaaag tatatttaat atatgtaaaa gagaaaagaa agtgtttcaa
     1141 aatgattatt tattatcagt gaaacacttt tttatttttt gatgtgtata tttactactt
     1201 tcaagagaag aatgtattaa aatcttaaga aaatct
//
LOCUS       DAEPXF010000369         1198 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain HB27
            SAMN13376389-rid12479423.denovo.377, whole genome shotgun sequence.
ACCESSION   DAEPXF010000369 DAEPXF010000000
VERSION     DAEPXF010000369.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13376389
            Sequence Read Archive: SRR10518432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1198)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1198)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001069624.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001069624.1
            Genome Coverage       :: 315x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/15/2021 01:54:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,165
            CDSs (total)                      :: 4,076
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 89
            rRNAs                             :: 10, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 10 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 19 of 69
            Pseudo Genes (incomplete)         :: 44 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 10 of 69
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1198
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13376389-rid12479423.denovo.377"
                     /strain="HB27"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="China: Hebei"
                     /collection_date="2017"
     gene            complement(343..834)
                     /locus_tag="KRL67_003948"
     CDS             complement(343..834)
                     /locus_tag="KRL67_003948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbM family protein"
                     /protein_id="HBH1524726.1"
                     /translation="MGMIGCYTKISEENVFKLQQTKENLQDFVFEDANENSTINIDKA
                     WHAIHFTLTGCPFGGDDENIFSKLVLSGNILMEIDGEFPVMLITANDVKKLSKAMNSL
                     EEQAFRKRFNINEMLENNIYPVMNDESEDDFFEYVWANLIELKKFIQEASDDGLAVIF
                     FIM"
BASE COUNT          460 a          164 c          110 g          464 t
ORIGIN      
        1 gaagttataa tttaacatat atttatttta tttgtgagtc tattttatat ataatgaaaa
       61 atgtgataag tatccaaaag aattactgga tataataaga tagggtattt agccttttaa
      121 gataaataaa aatatctgca actatatatt gcaaaaattt aaaactaatt tatatatgct
      181 aatataaata tttataaaga cataagacaa atttacctac agatttaaat ttaacttata
      241 tattttaaaa ggctaatata atatgtattt tcttctatac aatcaaaagg cttcatatct
      301 aagatatgtg atgacattaa tattcacaat ttttagtaat tatcacatta taaaaaatat
      361 tacagctagt ccatcatcag atgcttcttg tataaacttt ttaagctcaa ttagatttgc
      421 ccatacatat tcaaaaaaat catcttcact ctcatcattc ataacaggat aaatattatt
      481 ttctaacatc tcattaatat taaatctttt tctaaatgct tgttcttcca aactgttcat
      541 tgcttttgat agttttttta catcatttgc tgtaatcaac atgacaggaa attctccatc
      601 aatttccata agtatgtttc cacttagtac taatttgcta aatatattct catcatctcc
      661 accaaaagga caacctgtca aagtaaagtg aatggcatgc catgctttat caatattgat
      721 agtactattt tcgttggcat cttcaaaaac aaaatcttgt aaattttctt ttgtttgctg
      781 taatttaaaa acattctctt cacttatttt agtataacat cctatcatcc ccataattac
      841 attcctccta agtagcgttt ttttaattgt aacttataat taaacttagt tacaataaaa
      901 tatattgaga aaagtattat ttcatcttaa atttaaatac ctatattatc aactaaaatt
      961 ctgtgtttat agcgtaagta ttattatttt ttcctaaaaa ttaaatgaat cagaagttct
     1021 ataggcaata ttgactataa ataagaacta ttacttttaa aaaaaaattt aagaaattat
     1081 actgtagaat catgctctac ttattagctt caaataaaaa ataaatcatg ttctcaaatt
     1141 aaataataaa taacaataaa tttaaatttt aaactataag agtttctgaa gtgttaat
//
LOCUS       DAEPXF010000370         1168 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain HB27
            SAMN13376389-rid12479423.denovo.378, whole genome shotgun sequence.
ACCESSION   DAEPXF010000370 DAEPXF010000000
VERSION     DAEPXF010000370.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13376389
            Sequence Read Archive: SRR10518432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1168)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1168)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001069624.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001069624.1
            Genome Coverage       :: 315x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/15/2021 01:54:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,165
            CDSs (total)                      :: 4,076
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 89
            rRNAs                             :: 10, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 10 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 19 of 69
            Pseudo Genes (incomplete)         :: 44 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 10 of 69
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1168
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13376389-rid12479423.denovo.378"
                     /strain="HB27"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="China: Hebei"
                     /collection_date="2017"
     gene            257..>1168
                     /locus_tag="KRL67_003949"
     CDS             257..>1168
                     /locus_tag="KRL67_003949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBH1524727.1"
                     /translation="MIAVEKAYKFRMYPNKKQQELINKTFGCCRFVYNKYLAKRIEAY
                     KNNKETFTYKQCSSDLTNLKKELKWLKEPDKFSLQNALKDLDNAYKKFFKEKAGFPKF
                     KSKKINRFSYKTNFTNGNIMYCGQHIKLPKLGMVKVRDKQVPKGRILNATISKEPSGR
                     YYVSLCCTDVDIEAFKNTNNQIGLDLGIKEFCISSCGDFIENPKYLKKSLSKLAKLQR
                     ELSRKTIGSLNRNKARLKVARLQEHIANQRKDFLQKLSTKLIKENDIICIEDLQVKNM
                     IRNHKLSRLISDVSWSEFIRQLEYKANW"
BASE COUNT          510 a          129 c          170 g          359 t
ORIGIN      
        1 gaagttcata agagaactgc ataggtagta gcgaacttat gtgaacgaca acctcaaaaa
       61 tgataaaagt taagggctta tggttcttac atatgttatt taaaattaaa tataaaacct
      121 tctaaataat tgttttaaat aattacattt gttaacatta ttataggtga ttaataaatt
      181 attcaccaca gcattatata atatattaaa tatgataaaa tcattaaaaa gtgtatcaaa
      241 aaacaattaa agtgaggtga tagcagtgga aaaagcttat aagtttagaa tgtatccaaa
      301 taaaaaacaa caagagttaa ttaacaaaac ttttggatgt tgtagatttg tatataataa
      361 gtatcttgct aaaagaatag aagcttataa aaacaataaa gaaactttta cttataaaca
      421 gtgtagttct gatttaacca atttaaaaaa agaattaaaa tggcttaagg aacctgacaa
      481 attttcacta caaaacgcct taaaagattt agataatgca tataaaaaat tctttaaaga
      541 aaaggcagga tttcctaaat ttaaatcaaa gaaaattaat agattttcat ataaaactaa
      601 ctttacaaat ggaaacatta tgtattgtgg tcaacatata aaactaccta aacttggtat
      661 ggttaaagta agggataaac aagtacctaa aggtagaata cttaatgcta ctatatctaa
      721 agaaccaagt ggtagatatt atgtatcatt atgctgtact gatgtagata ttgaagcatt
      781 taaaaatact aataatcaga taggtttaga tttaggaatt aaagaatttt gtatctcaag
      841 ttgtggagac tttatagaaa atcctaagta tcttaagaag tcattaagca aacttgctaa
      901 attacaaaga gaattatcga gaaaaacaat cggtagttta aatagaaata aagcaagatt
      961 aaaagttgca agacttcaag aacatatagc caatcaaaga aaggattttc tacaaaaact
     1021 atctactaaa ttaataaaag agaacgatat tatttgcata gaagatttac aagtaaagaa
     1081 tatgattaga aatcataaac tatctcgctt aatttcagat gtatcatggt cagagtttat
     1141 tcgccaatta gaatacaagg ctaattgg
//
LOCUS       DAEPXF010000371         1159 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain HB27
            SAMN13376389-rid12479423.denovo.379, whole genome shotgun sequence.
ACCESSION   DAEPXF010000371 DAEPXF010000000
VERSION     DAEPXF010000371.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13376389
            Sequence Read Archive: SRR10518432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1159)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1159)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001069624.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001069624.1
            Genome Coverage       :: 315x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/15/2021 01:54:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,165
            CDSs (total)                      :: 4,076
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 89
            rRNAs                             :: 10, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 10 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 19 of 69
            Pseudo Genes (incomplete)         :: 44 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 10 of 69
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1159
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13376389-rid12479423.denovo.379"
                     /strain="HB27"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="China: Hebei"
                     /collection_date="2017"
     gene            71..>1159
                     /locus_tag="KRL67_003950"
     CDS             71..>1159
                     /locus_tag="KRL67_003950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH1524728.1"
                     /translation="MKLKSKISILIIFLTLAIYFSIALLNNEDNLKIKTIKGDPNELD
                     YININFGYALNGYINSYYTINGSNKTKKTELIKLQKDSKNTNNLIDVSSGSDLIAHVG
                     FEMNDDSKDINFVIDEKKEDLEKGVFPSEGSDKEIEYEKLDMNNFTRTRYKVDKSTDD
                     YYANILTSKRYKKYLYVSVLYATSDSSENNKEHSSTIDIIKINPENKKIKTIKSINLS
                     NYFSEKYQDIETIGVSYASKYEDKMYVLVNVLNTSIGDNSNSKFHLLEYNLKENKESI
                     MELKTDSKMALAKVKLEDNKLYMVMSNDYDNSSITELNLLTYNILDKSLDEDKYVLDN
                     KEKRGIRSIEIDSNKIVAYTSRLNSILSW"
BASE COUNT          529 a           94 c          168 g          368 t
ORIGIN      
        1 taatgtttat tctaaatgga atatcttatt tgctaattaa aaagaaagta tctctttaag
       61 gaggggtggt atgaaattaa aatctaaaat atctatactt attatatttt tgactttagc
      121 aatatatttt agtatagctt tattaaataa tgaagataat ttaaagataa aaactataaa
      181 aggtgaccct aatgaattag actatataaa cataaatttt ggatatgctt tgaatggata
      241 tataaattca tattatacaa taaatggaag taacaaaacc aaaaagactg aattaataaa
      301 actacaaaag gatagtaaaa acacaaataa tttaattgat gtatctagcg gttcagattt
      361 aattgctcat gttggatttg aaatgaatga tgattcaaaa gatataaact ttgtaataga
      421 tgagaaaaaa gaagatttag aaaaaggtgt atttccatca gaaggaagtg ataaagaaat
      481 tgaatatgaa aaattagata tgaataattt tacaaggaca agatataaag tagataaaag
      541 tacagatgat tattatgcaa atatactaac atcaaaaaga tataaaaaat acttatatgt
      601 atctgtactt tatgctacaa gtgattcatc agaaaataac aaagaacatt caagtactat
      661 agatataata aaaattaatc cagaaaataa aaaaatcaag actataaaaa gtataaattt
      721 aagtaattat ttttccgaaa aataccaaga catagagact ataggtgtaa gttatgctag
      781 caaatatgag gataagatgt atgttttagt aaatgtacta aatacaagta taggtgataa
      841 tagtaattct aaatttcatt tacttgagta taatcttaaa gaaaataagg aaagcataat
      901 ggaattaaaa actgatagca aaatggcttt agcaaaagta aagttagaag ataataaatt
      961 atatatggta atgtcaaatg attatgataa tagttcaatt acagagttaa atttacttac
     1021 ctataatata ttagataaaa gtttagatga agataaatat gttttagata ataaagaaaa
     1081 aagaggcatt agaagtattg aaattgattc aaataaaatc gttgcatata catcaaggct
     1141 aaattctata ttatcttgg
//
LOCUS       DAEPXF010000372         1134 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain HB27
            SAMN13376389-rid12479423.denovo.380, whole genome shotgun sequence.
ACCESSION   DAEPXF010000372 DAEPXF010000000
VERSION     DAEPXF010000372.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13376389
            Sequence Read Archive: SRR10518432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1134)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1134)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001069624.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001069624.1
            Genome Coverage       :: 315x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/15/2021 01:54:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,165
            CDSs (total)                      :: 4,076
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 89
            rRNAs                             :: 10, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 10 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 19 of 69
            Pseudo Genes (incomplete)         :: 44 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 10 of 69
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1134
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13376389-rid12479423.denovo.380"
                     /strain="HB27"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="China: Hebei"
                     /collection_date="2017"
     gene            248..952
                     /gene="cwlD"
                     /locus_tag="KRL67_003951"
     CDS             248..952
                     /gene="cwlD"
                     /locus_tag="KRL67_003951"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase CwlD"
                     /protein_id="HBH1524729.1"
                     /translation="MRKYIKHIIFSFAMICLAVVSIFEIKNISEDVIKYMPVTNKTII
                     LDAGHGGIDPGALNKDKSTSEKDINLAITLKLRELIESSGGLVILTREDDSSLYKEEN
                     NKTTRQKYNENLKNRKEIISNSNANMFVSIHLNAFEQSKYYGAQTFYPKDKQDSKELS
                     KCIQEELKRVVDKTNNREVKPRDDIYLLKENNIPSVLIECGFLSNEKECKLLTDETYQ
                     EKIAWAIYIGIQKYLS"
BASE COUNT          496 a          117 c          175 g          346 t
ORIGIN      
        1 tttaacaata taaattctga atatacgtta tcaaagttta taatataaac taagtataat
       61 ttattgtata gtgttgaatg ttagataaac atagtgagtt aaataggggc aggaatataa
      121 tatcattaca taataatata ttaataccaa taaataaaga tatatcaata ttaaaaaaat
      181 taaatgtttg tactaatatc tggaatatta tagtgttttt ataagtatac atatacaggg
      241 tgatgttgtg agaaagtaca taaaacatat aatttttagt tttgcaatga tatgtctagc
      301 agtagtatcc atatttgaaa ttaaaaatat ttctgaagat gttatcaagt atatgccagt
      361 aacaaacaaa actataattt tagatgctgg tcatggaggc attgatccag gtgcattaaa
      421 taaggataag agtacatctg aaaaggatat taacctagca ataacactta agcttagaga
      481 gcttatagaa tcaagtggcg gtcttgtaat attaactcga gaagatgata gtagccttta
      541 taaagaagaa aacaataaaa ctacaaggca aaaatataat gagaatttga aaaatagaaa
      601 agaaataata tctaattcta acgccaatat gtttgtctct atacatctta atgcatttga
      661 acaatcgaaa tactatgggg ctcaaacttt ttatcctaaa gacaaacaag acagtaaaga
      721 attatcgaag tgtattcaag aagaacttaa aagagtagta gataagacaa acaacagaga
      781 agtcaaacca agggatgata tatatctttt gaaggaaaat aatattccat cagtactgat
      841 agaatgtggt tttttatcaa atgaaaaaga gtgtaaactc ttaactgatg aaacatatca
      901 agaaaaaata gcatgggcaa tctacatagg aatacaaaaa tatttaagtt gaaataacta
      961 taaaaatagg tctaaatatg taaatagctt gtattaaagc agttaaaatg cattaatata
     1021 ggctattttt attttgacaa aaaaatattt aaaataaaag ttaaaaaatt gttgacttag
     1081 aataatatag atgatattat atatgagtgc ccaaaaggag caccaaaata agac
//
LOCUS       DAEPXF010000373         1105 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain HB27
            SAMN13376389-rid12479423.denovo.381, whole genome shotgun sequence.
ACCESSION   DAEPXF010000373 DAEPXF010000000
VERSION     DAEPXF010000373.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13376389
            Sequence Read Archive: SRR10518432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1105)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1105)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001069624.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001069624.1
            Genome Coverage       :: 315x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/15/2021 01:54:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,165
            CDSs (total)                      :: 4,076
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 89
            rRNAs                             :: 10, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 10 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 19 of 69
            Pseudo Genes (incomplete)         :: 44 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 10 of 69
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1105
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13376389-rid12479423.denovo.381"
                     /strain="HB27"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="China: Hebei"
                     /collection_date="2017"
     gene            158..>1105
                     /locus_tag="KRL67_003952"
     CDS             158..>1105
                     /locus_tag="KRL67_003952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HBH1524730.1"
                     /translation="MKINKKFNRVDMQVSILTAIIVIISSSCVYFFNYTLTYKNMIYS
                     LSERSKHIYEYVERNIDKNTFTEINSKEDQSKKSYKSSKILLESVKNTTGVMYLYTAK
                     KTKDGSLVYVVDGLNSSRSDFRNAGDLIEKEICGDINKALDNHILLPKKIKSTSWGYI
                     FISYFPIHNDVGKVVGVIGIEFEAKHQYNTFKFISIVTPIIALLTCLISALIAVILFK
                     RISNPTYTDFANTDYLTGLKNRNAFEIDMNNLNNSNLKNLISIISIDLDGLKKINDKF
                     GHQAGDLYIKQASKIIQYVINKKYIVYRIGGDEYIIILKN"
BASE COUNT          460 a          114 c          151 g          380 t
ORIGIN      
        1 aataataaaa aaactatatt tgaatacttt aagcatgttt actaattgta atttccacac
       61 aaatttgata atatatgaat atattatggt gaaatttata gatatatatt aattatgggg
      121 agattgtggg aggttctttt cacaagacat ttaagctatg aaaataaata aaaaattcaa
      181 tagagttgat atgcaggttt caatacttac tgcaattatt gtaataatat cttcctcatg
      241 tgtatatttt tttaattata cactaactta taagaacatg atatactctt taagtgagag
      301 gtcaaaacat atctatgaat atgtagaaag aaatattgat aaaaatacct ttactgaaat
      361 taattctaaa gaagaccaat caaaaaagtc ttataaaagt tctaaaatcc tattagaatc
      421 tgtaaaaaat actactggag ttatgtatct atatactgct aaaaaaacaa aagatggatc
      481 actagtatat gttgttgatg gtttaaattc atctagaagt gattttagaa atgctggtga
      541 tttgatagaa aaagagattt gcggagacat taacaaagca ttagataatc atatattgtt
      601 acctaaaaaa attaaatcaa catcttgggg atacatattt atatcgtatt ttcctattca
      661 taatgatgtt ggtaaggttg ttggagtaat aggaattgaa tttgaagcaa aacatcaata
      721 caatacattt aaatttataa gtatagttac accaataata gcattattaa cttgtctaat
      781 atcagcattg attgcagtga ttctatttaa aagaatatct aatcctacat atactgattt
      841 tgctaataca gattatttaa caggactaaa aaatagaaat gcttttgaaa tagatatgaa
      901 taacttaaac aattcaaatc taaagaatct aatatctatc atatcaatag atttagatgg
      961 tttaaaaaag attaatgata aatttggcca tcaagcagga gacttatata ttaagcaagc
     1021 aagtaaaatt atacaatatg taataaataa aaaatatata gtttatcgta ttggaggtga
     1081 tgaatacata attattctaa aaaat
//
LOCUS       DAEPXF010000374         1095 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain HB27
            SAMN13376389-rid12479423.denovo.382, whole genome shotgun sequence.
ACCESSION   DAEPXF010000374 DAEPXF010000000
VERSION     DAEPXF010000374.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13376389
            Sequence Read Archive: SRR10518432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1095)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1095)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001069624.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001069624.1
            Genome Coverage       :: 315x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/15/2021 01:54:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,165
            CDSs (total)                      :: 4,076
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 89
            rRNAs                             :: 10, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 10 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 19 of 69
            Pseudo Genes (incomplete)         :: 44 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 10 of 69
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1095
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13376389-rid12479423.denovo.382"
                     /strain="HB27"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="China: Hebei"
                     /collection_date="2017"
     gene            24..281
                     /locus_tag="KRL67_003953"
     CDS             24..281
                     /locus_tag="KRL67_003953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH1524731.1"
                     /translation="MIIIRSQDKTNLMHINQIKIDGSQVYAVFESKIDTVKIGDYENN
                     TRAIQVLDNIQNFIENGTKYDYITSNKVRYNVNKIFQMPAK"
     gene            complement(390..1070)
                     /locus_tag="KRL67_003954"
     CDS             complement(390..1070)
                     /locus_tag="KRL67_003954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HBH1524732.1"
                     /translation="MIWTFYSFICVILPCLIYQIVVIKRKNLKVEKNVVIHLVWVYIF
                     LLYIYLALSKAGIGSIWDIGRYSSLFRVDEINLIPFDSVGILTYILNIIMFMPLGFLL
                     PLIWKNFRNIINVLLTGLGFSLAIEFCQLFNLRATDIDDLMMNTLGAILGYFIWVGVN
                     NLFNLIKRKDIFPSIYKNIIQDEIAITIDDDISKKITSLYKNEAIIYLILAVLGEFLL
                     FNWSIIPY"
BASE COUNT          449 a          139 c          126 g          381 t
ORIGIN      
        1 attaagtaaa tggaggaatt gatatgataa taattagaag tcaagataaa actaatttaa
       61 tgcatataaa ccaaattaaa atagatggta gtcaagttta tgcagtattt gaaagtaaaa
      121 ttgatactgt aaaaatagga gattatgaaa ataatacaag agctatacaa gtgttggaca
      181 atatacaaaa cttcattgaa aatggaacaa aatatgatta tataacatca aataaggtca
      241 gatacaatgt taataaaata tttcagatgc cagctaaata gtgagtgtgt attttagttt
      301 aataactaaa gcccactttc ttttgtagta tgaaagaggg ctttacatat ttgtaaatat
      361 cgaagtacat ttgtttatca ataaattatt taataaggta taatggacca attaaacaat
      421 aaaaattctc ctaaaacagc aagtattaaa tatataatag cttcattttt gtaaagagag
      481 gtaatttttt tgcttatatc atcatcaatt gtaatagcaa tttcatcttg gataatattt
      541 ttatatatgg atggaaaaat atcttttctc tttataagat taaataaatt atttacacct
      601 acccatataa aatatcctaa aatagcacct aatgtgttca tcattaaatc atcaatatct
      661 gtagctctta agttaaacaa ttgacaaaat tcaattgcta atgaaaaacc taatccagtt
      721 aataaaacat tgataatatt tctaaaattt ttccaaatta aaggtagtaa aaatcctaaa
      781 ggcataaaca taattatgtt taaaatatat gttaaaatgc caactgagtc aaaaggtatt
      841 agatttattt catcaactct aaaaaggcta ctataccttc ctatatccca tatgctacca
      901 atgccagctt tactaagtgc taggtatata tataataaaa aaatatatac ccatactaaa
      961 tgtatcacta cattcttttc aactttcaaa ttttttcttt ttataacaac tatttggtat
     1021 attaagcatg gtaatataac gcaaataaat gaataaaatg tccatatcat tttattttgt
     1081 cccctttcaa attta
//
LOCUS       DAEPXF010000375         1087 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain HB27
            SAMN13376389-rid12479423.denovo.383, whole genome shotgun sequence.
ACCESSION   DAEPXF010000375 DAEPXF010000000
VERSION     DAEPXF010000375.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13376389
            Sequence Read Archive: SRR10518432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1087)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1087)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001069624.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001069624.1
            Genome Coverage       :: 315x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/15/2021 01:54:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,165
            CDSs (total)                      :: 4,076
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 89
            rRNAs                             :: 10, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 10 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 19 of 69
            Pseudo Genes (incomplete)         :: 44 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 10 of 69
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1087
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13376389-rid12479423.denovo.383"
                     /strain="HB27"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="China: Hebei"
                     /collection_date="2017"
     gene            complement(207..1076)
                     /locus_tag="KRL67_003955"
     CDS             complement(207..1076)
                     /locus_tag="KRL67_003955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HBH1524733.1"
                     /translation="MVIIKAIELYKKLDLEFNIKNINDDWSFMNFENDFITPEFRKKY
                     IGLVLDNAQNINKVYTTTFPDKEIIKQVIDKDEKDILIFSHHAMGYIASDEGFPFHDI
                     PLSYMEEMKNRRISFYVLHSPLDNYSDYSTSVSFAKALGLEIVKPFCKYDDKIEVGVI
                     CKTSLKSIEEIKELIKKSVGHDIKLYDYGDSVLKDGLVAIAAGGGSYPFVAREVAELG
                     INLYITGFTKPLKHFEPVLEFHQIAKDNLINVIGATHYSTEKFACMGMVDYFKNLGLE
                     SEFLQGKYYLEDL"
BASE COUNT          400 a          172 c          111 g          404 t
ORIGIN      
        1 tacatcaaaa aataaactaa aaaatatcga aaaataattt aaaagacatc aaaactaaag
       61 cttctctaat cagcaattaa aagtctattt tcttagttta aaaaataaag atagattttt
      121 tcaaaacact ggataaatgg gtaaaaaaat aaacagttag taaaacaaat ttttactaac
      181 tgcttatttt tattgtttaa tatatcctat aaatcctcta agtaatactt tccttgcaaa
      241 aattctgatt ctaaacctag atttttaaaa taatcgacca tgcccataca tgcaaatttt
      301 tctgtagaat aatgagtagc acctataaca ttaattaaat tatcttttgc tatttgatgg
      361 aactctaata ctggttcaaa atgttttaaa ggtttagtaa atccagtaat atatagattt
      421 atgccaagtt cagcaacttc tcttgctaca aatggataac ttcctccccc tgctgcaata
      481 gcaactaaac catcttttaa tacagaatct ccataatcat acaatttaat atcatgacca
      541 acagattttt taattaattc cttaatctct tctattgatt ttagtgatgt cttacatata
      601 actcctacct caattttatc gtcatatttg caaaatggtt tgactatttc taaacctagg
      661 gcttttgcaa aactaacaga agttgaatag tcactatagt tgtctagtgg agaatgtaat
      721 acataaaaag aaattctacg atttttcatc tcttccatat aagatagtgg tatgtcgtga
      781 aatggaaaac cttcatctga tgctatgtat cccattgcat gatgtgaaaa aattaaaata
      841 tctttttcat ctttatctat aacctgttta attatctctt tatcaggaaa agtagtcgtg
      901 tatactttat taatattttg agcattatct aatactaatc ctatgtactt ttttctaaat
      961 tctggagtga taaaatcatt ttcaaaattc ataaaactcc aatcatcatt aatattttta
     1021 atattaaatt ctaaatctaa ttttttataa agttcaattg ccttaataat aaccactcct
     1081 ttataat
//
LOCUS       DAEPXF010000376         1036 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain HB27
            SAMN13376389-rid12479423.denovo.384, whole genome shotgun sequence.
ACCESSION   DAEPXF010000376 DAEPXF010000000
VERSION     DAEPXF010000376.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13376389
            Sequence Read Archive: SRR10518432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1036)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1036)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001069624.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001069624.1
            Genome Coverage       :: 315x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/15/2021 01:54:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,165
            CDSs (total)                      :: 4,076
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 89
            rRNAs                             :: 10, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 10 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 19 of 69
            Pseudo Genes (incomplete)         :: 44 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 10 of 69
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1036
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13376389-rid12479423.denovo.384"
                     /strain="HB27"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="China: Hebei"
                     /collection_date="2017"
     gene            204..905
                     /locus_tag="KRL67_003956"
     CDS             204..905
                     /locus_tag="KRL67_003956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4253 domain-containing protein"
                     /protein_id="HBH1524734.1"
                     /translation="MGIEDLYDFECVPLNNEIDIDVLFEKMIEDGKEKEYTPIIVIDE
                     KVGLLRKNIDMITKEYGSLENYREYCLTKYNEIDVNQFFDFRKSEISELLKELIDLED
                     DFYDYRHFRPIMNVDIFHEYNKVYIAKIPTIKPYEVFAYIPIGGFNDCPRDEEHIAIA
                     KYWYEKHGIFPIAIGCDTVQYSVKNLTKDGKKFDDLCIELVFYCEDIIIQGYETLKAL
                     KDVLKRSTIVLFWWD"
BASE COUNT          406 a           90 c          173 g          367 t
ORIGIN      
        1 atcatattta tataagggga ttgtcataaa aaatatcttg ttcatacagt tttatataga
       61 aaaacgaaat gaatttttgc tttatataag tatattttgt aaattgcctc tatatttata
      121 gtttaaataa tagtggtatt aaaaatctga caataaaaat aaaactttca aaaaattaaa
      181 tctaaaaaga ataggagata aatatgggta ttgaagattt atatgatttt gaatgtgttc
      241 ctttgaacaa tgagattgat attgatgttt tatttgaaaa gatgatagaa gatggaaaag
      301 aaaaagaata tacaccaatt atagtcattg atgaaaaagt agggctatta agaaaaaata
      361 tagatatgat tactaaagag tatggttctc ttgaaaacta tagagagtat tgtttaacaa
      421 aatataatga aattgatgta aatcaatttt ttgattttag gaagagtgag atttcagaat
      481 tattaaagga gctaattgat ttagaagatg atttttatga ttatagacat tttagaccaa
      541 ttatgaatgt agatatattt catgaatata acaaagttta catagctaaa attccaacaa
      601 ttaaaccata tgaagtattt gcatacatac ctattggtgg atttaatgat tgtcctagag
      661 atgaagaaca tatagctatt gcaaaatatt ggtatgaaaa gcatggaatc tttccaatcg
      721 caattggatg tgatacagtt caatattctg taaaaaattt aactaaagat ggtaaaaaat
      781 ttgatgactt atgtattgaa ttagtatttt attgtgagga tattattata caagggtatg
      841 aaaccttaaa ggctcttaag gatgtattaa aacgctctac aatagtgctt ttttggtggg
      901 attgatattc tatcatttag atagtattaa attgttagtc accaagaatc cccttgcttt
      961 agttatgggg agtgtgtcaa actaggtttt tatacacagt atagcacagc aatatgttta
     1021 aattataaag gttaaa
//
LOCUS       DAEPXF010000377          979 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain HB27
            SAMN13376389-rid12479423.denovo.385, whole genome shotgun sequence.
ACCESSION   DAEPXF010000377 DAEPXF010000000
VERSION     DAEPXF010000377.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13376389
            Sequence Read Archive: SRR10518432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 979)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 979)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001069624.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001069624.1
            Genome Coverage       :: 315x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/15/2021 01:54:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,165
            CDSs (total)                      :: 4,076
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 89
            rRNAs                             :: 10, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 10 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 19 of 69
            Pseudo Genes (incomplete)         :: 44 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 10 of 69
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..979
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13376389-rid12479423.denovo.385"
                     /strain="HB27"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="China: Hebei"
                     /collection_date="2017"
     gene            199..>979
                     /locus_tag="KRL67_003957"
     CDS             199..>979
                     /locus_tag="KRL67_003957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mrp/NBP35 family ATP-binding protein"
                     /protein_id="HBH1524735.1"
                     /translation="MADCNSCPSKGNCNSQSNCSIENNPNNKFGKIIGVMSGKGGVGK
                     STVTALLANKLNKMGYKVGILDSDITGPSIPRLMGVKNVKAYSDGSYIYPVENSNNIK
                     VMSINLMIDDENEPVVWRGPLLGGVVKQFYTDVLWEELDYLLIDMPPGTGDVALTVMQ
                     SIPISGIVMVSVPQDLVSMIVSKAVNMAKKMNINVLGVIENMSYIQCPDCSKKIKLFE
                     GESTEKFLDDLDLELLGELPMTKEIIDITHNGVTEISDDLDS"
BASE COUNT          358 a          106 c          173 g          342 t
ORIGIN      
        1 ataactaaat tgaaataatc actttttcaa gaatgttata atttttttat tgtattcttt
       61 ttgctatagt ctataaataa attaatagaa aatcttctaa gttactttta aataatgact
      121 ttatgattca attgtactaa aatagtatat gtaggttaca aataatctaa taaatattaa
      181 agtttggagg taatagatat ggcagattgt aattcatgtc catcaaaggg aaattgtaat
      241 agtcaatcaa attgttctat tgaaaataac cctaacaata aatttggaaa aataattggt
      301 gttatgagtg ggaagggtgg agttggaaaa tcaactgtaa ctgctttact tgctaataag
      361 ctaaacaaaa tgggatataa ggttggtata ttagattcag atataactgg accaagtatt
      421 cctagactta tgggtgtaaa aaatgtaaaa gcttattctg atggctctta tatttaccct
      481 gtagaaaact caaataacat aaaagtaatg tctataaacc ttatgataga tgatgagaat
      541 gaacctgttg tttggagagg acctttactt ggaggagttg taaagcaatt ttatactgat
      601 gtactttggg aagaattaga ctatttatta atagatatgc ctcctggtac tggagatgtt
      661 gccttaacgg ttatgcaatc tattcctata agtggaatag ttatggtttc agtacctcaa
      721 gatttggtat ctatgattgt atcaaaagct gttaatatgg ctaaaaaaat gaacataaat
      781 gtccttggag ttattgaaaa tatgagttat atccaatgcc ctgattgttc taaaaaaatc
      841 aagttatttg aaggagaatc tactgaaaag tttttagatg atttagactt agagctttta
      901 ggagaacttc ctatgactaa agaaattata gatattactc ataatggagt tacagaaatt
      961 agtgatgatt tagattcaa
//
LOCUS       DAEPXF010000378          970 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain HB27
            SAMN13376389-rid12479423.denovo.386, whole genome shotgun sequence.
ACCESSION   DAEPXF010000378 DAEPXF010000000
VERSION     DAEPXF010000378.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13376389
            Sequence Read Archive: SRR10518432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 970)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 970)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001069624.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001069624.1
            Genome Coverage       :: 315x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/15/2021 01:54:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,165
            CDSs (total)                      :: 4,076
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 89
            rRNAs                             :: 10, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 10 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 19 of 69
            Pseudo Genes (incomplete)         :: 44 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 10 of 69
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..970
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13376389-rid12479423.denovo.386"
                     /strain="HB27"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="China: Hebei"
                     /collection_date="2017"
     gene            22..531
                     /gene="vanZ1"
                     /locus_tag="KRL67_003958"
     CDS             22..531
                     /gene="vanZ1"
                     /locus_tag="KRL67_003958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycopeptide resistance protein VanZ1"
                     /protein_id="HBH1524736.1"
                     /translation="MKSRKHNITKGLFIVYIIILTWIILFKLQFDISSLETMNLRSIN
                     LVPFAGSLIINNRVDISEIILNVVIFVPFGIYVCMLKEEWSFIKKVIPIFITSLAFET
                     LQYIFALGASDITDLIGNTLGGIIGIAVFMLLSKIFKNNTIKIINVLALIVTIIVVLF
                     LGLVIFANL"
     gene            complement(633..827)
                     /locus_tag="KRL67_003959"
     CDS             complement(633..827)
                     /locus_tag="KRL67_003959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase"
                     /protein_id="HBH1524737.1"
                     /translation="MSVIVMCSRCGKEIDKYRNKYITYYEKNSNAEIKVCINCALDLL
                     EKTRENQELNIQEFQDTIIL"
BASE COUNT          356 a          115 c          120 g          379 t
ORIGIN      
        1 aatatagtaa ggaggtaata aatgaaatct agaaaacata atattacaaa aggattattt
       61 atagtttaca taattatttt aacatggata atactattca agttgcaatt tgatatctca
      121 tcccttgaaa ccatgaatct tagaagcata aacttagtac cttttgctgg gtcacttata
      181 attaataata gagtagatat atcagagatt atattaaatg tggttatatt tgtaccattt
      241 gggatatatg tgtgtatgct aaaagaagaa tggagcttta taaaaaaagt tatacctatt
      301 tttataacta gtttggcatt tgaaacactt caatatatat ttgcactagg tgcaagtgat
      361 ataacggatt tgataggaaa cacactagga ggaatcatag gaatagctgt ttttatgcta
      421 ttatcgaaaa tatttaagaa taataccata aaaattatca atgtattggc attgattgtg
      481 acaattattg ttgtattatt tttaggatta gttatttttg caaacttata gaaataagta
      541 tatatgttta gcttaaaata aaacgtaaga tacaattaca tataaaatta tttacacata
      601 aaaagcacct atacaggtgc tttcctaaat tattaaagaa ttattgtatc ttgaaactct
      661 tgtatgttca attcttgatt ttctcttgtt ttttctaata aatctaacgc acaatttata
      721 caaactttaa tttctgcatt gctatttttc tcataataag ttatatattt atttctatat
      781 ttatctattt cttttccaca acgactacac attactatta cgctcatatc ttagttcctc
      841 catctctaat tattcttaat atattcaata gtatcacttt tctgaaaata atgattgtta
      901 aaactgtatc aaatttagat ttacttttta ttataatgca ttttaataaa aaaataaagg
      961 ttatgggtaa
//
LOCUS       DAEPXF010000379          967 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain HB27
            SAMN13376389-rid12479423.denovo.387, whole genome shotgun sequence.
ACCESSION   DAEPXF010000379 DAEPXF010000000
VERSION     DAEPXF010000379.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13376389
            Sequence Read Archive: SRR10518432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 967)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 967)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001069624.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001069624.1
            Genome Coverage       :: 315x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/15/2021 01:54:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,165
            CDSs (total)                      :: 4,076
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 89
            rRNAs                             :: 10, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 10 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 19 of 69
            Pseudo Genes (incomplete)         :: 44 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 10 of 69
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..967
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13376389-rid12479423.denovo.387"
                     /strain="HB27"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="China: Hebei"
                     /collection_date="2017"
     gene            complement(196..912)
                     /gene="dapD"
                     /locus_tag="KRL67_003960"
     CDS             complement(196..912)
                     /gene="dapD"
                     /locus_tag="KRL67_003960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-acetyltransferase"
                     /protein_id="HBH1524738.1"
                     /translation="MVNLNDAYEIARYIKEVKKKTPVKVYVNTNSLHFQSTDKFKVFG
                     SNGSYIFVGDYEDIMEVLDKHGNDITDTHVEYDRRNSAIPLKNTLSEHARIEPGAIIR
                     DMVTIEKNAVVMMGAVINIGAVIGEGSMVDMNAVIGARGTLGKNVHLGAGAVVAGVLE
                     PPSATPVIVEDDVLIGANAVILEGVRIGKGAVVAAGAVVTTDVEAGAVVAGSPAKVIK
                     MKDEKTADKTKLMEDLRNLD"
BASE COUNT          332 a          175 c          106 g          354 t
ORIGIN      
        1 aataaaaatg atataagaat gaataagaga attgtaaaaa aataaaatga ttatcaataa
       61 aagataaaaa tattataaaa aagagggtgt ctctggtgaa ttaaattcat acagagacac
      121 ctttttaatt aattttttaa attagtatat tattgtttaa ttaatctttt taattaatag
      181 tatagtatta tttaattagt ctaaatttct aaggtcttcc ataagcttag ttttgtcagc
      241 cgttttttca tctttcattt ttataacttt agcaggagaa ccagcaacaa cggcaccagc
      301 ttctacatca gtagttacaa cagcaccagc tgcaacaaca gcacctttac cgattctaac
      361 accttcaaga ataactgcat tagctcctat taaaacatca tcttcaacta taacaggagt
      421 agcagaaggt ggctctaaaa caccagcaac aacagcacca gcacctaagt gaacattttt
      481 accaagagtt cctctagctc caataacagc attcatatct accatagaac cttcaccaat
      541 aacagctcct atatttataa cagctcccat cattacaaca gcattttttt ctatagtaac
      601 catgtctctt attattgcac ctggttcaat tcttgcatgt tcacttaaag tattttttag
      661 aggaatagca gaatttcttc tatcatattc tacgtgagta tctgttatat catttccatg
      721 tttatctaat acttccatta tatcctcata gtcacctaca aatatataag aaccattact
      781 tccaaatacc ttaaatttat cagttgattg gaagtgtaaa gaattagtat ttacatatac
      841 cttaacaggt gttttctttt taacttcttt tatatatctt gctatttcat aagcatcgtt
      901 taaatttacc atttgttaat tctccttgta tttcattata tttcttttat tatatactta
      961 tttatta
//
LOCUS       DAEPXF010000380          958 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain HB27
            SAMN13376389-rid12479423.denovo.388, whole genome shotgun sequence.
ACCESSION   DAEPXF010000380 DAEPXF010000000
VERSION     DAEPXF010000380.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13376389
            Sequence Read Archive: SRR10518432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 958)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 958)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001069624.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001069624.1
            Genome Coverage       :: 315x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/15/2021 01:54:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,165
            CDSs (total)                      :: 4,076
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 89
            rRNAs                             :: 10, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 10 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 19 of 69
            Pseudo Genes (incomplete)         :: 44 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 10 of 69
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..958
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13376389-rid12479423.denovo.388"
                     /strain="HB27"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="China: Hebei"
                     /collection_date="2017"
     gene            complement(114..845)
                     /locus_tag="KRL67_003961"
     CDS             complement(114..845)
                     /locus_tag="KRL67_003961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBH1524739.1"
                     /translation="MIRKKVYILISIAICDDESYMLNHLKENIELYMKQKNLLYNIEL
                     FNKVEDLLCNHEKFNIVFLDIQMEEINGMQAARKLRSYGNECFIIFVTVLNEFVFDAF
                     EVDAINYLIKPLSNEKLYKTLDKIVTKLEDNESNYITIQKGYSYWKLKLADILYCEVI
                     KRKIYVHLKKETVDYYETIENLERSLPTNFFRCHRSYIINLQYVYGYENGNAVMESGD
                     RIPVSRLRQQIFSKVMLEFMKNRRI"
BASE COUNT          350 a          149 c           78 g          381 t
ORIGIN      
        1 taaaattgat tagaaaatag tattgcacta ttaccattaa aattctatat acaataaaag
       61 agaataaaaa actgtacttt aaaaataaat cagaagaaaa taaattaatc atcttatatc
      121 ctcctatttt tcatgaactc aagcataact tttgaaaata tttgttgacg tagtctagaa
      181 acaggtattc tatctccact ttccataact gcattaccat tttcatatcc atatacatat
      241 tgtaaattga ttatataact acgatgacac ctaaaaaaat ttgttggtaa acttctctct
      301 aaattttcaa tagtctcata ataatctaca gtctctttct ttaaatgaac ataaatcttt
      361 ctttttataa cttcacaata taaaatatca gcaagtttta atttccaata tgaataacct
      421 ttttgtattg tgatataatt actttcatta tcctcaagtt ttgtaactat tttatccaaa
      481 gttttatata gtttttcatt agatagaggt tttatcaaat aatttatagc atctacttca
      541 aaagcatcaa atacaaattc atttaaaact gtaacgaaaa ttataaagca ctcattacca
      601 tatgacctta attttctagc tgcctgcatt ccgttaatct cttccatctg tatatctaaa
      661 aatacaatat taaacttttc atgattgcat agtaaatctt ctactttatt aaaaagctct
      721 atattatata ataaattctt ctgcttcatg tataattcta tattttcctt caaatgattt
      781 agcatatagg attcatcatc acaaatggca attgaaatca atatatacac cttctttctt
      841 atcattattc taattattat ataaagttaa ttttttctgt tcaattagtc tgatctaata
      901 tgttgaatat ttgttccaaa atgtaataat ctcacttttc atagtatatc taatttga
//
LOCUS       DAEPXF010000381          955 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain HB27
            SAMN13376389-rid12479423.denovo.389, whole genome shotgun sequence.
ACCESSION   DAEPXF010000381 DAEPXF010000000
VERSION     DAEPXF010000381.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13376389
            Sequence Read Archive: SRR10518432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 955)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 955)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001069624.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001069624.1
            Genome Coverage       :: 315x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/15/2021 01:54:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,165
            CDSs (total)                      :: 4,076
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 89
            rRNAs                             :: 10, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 10 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 19 of 69
            Pseudo Genes (incomplete)         :: 44 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 10 of 69
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..955
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13376389-rid12479423.denovo.389"
                     /strain="HB27"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="China: Hebei"
                     /collection_date="2017"
     gene            2..>955
                     /locus_tag="KRL67_003962"
     CDS             2..>955
                     /locus_tag="KRL67_003962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CorA family divalent cation transporter"
                     /protein_id="HBH1524740.1"
                     /translation="MYILNLNTRETIEDFRDKFYVAENSYLILSAPKNLKLLKETLDI
                     DEITFNDCLKFDEITKLDLFDNYDFLSLNTFELRDGEAVIEEVNMYLSDNFILVVVNE
                     EHFLFEFVKNIILKNSQLEKNPVINLFKINYLILREVIKNGFESLEKVEELILQIEDE
                     MMDNINKNHVSRISDVRGLTRIIVKNTRPLLYIGDRIVKENIRYLKYSNVKKYNLENF
                     QGIDFGIDKLYNFALSTRELADKLLDIYSSRVGEKTNNLITKLTLLTAISAPLTIITG
                     IYGMNFRYMPELNWIYGYPATLFFMLCIIFVGIIIFKIKKLL"
BASE COUNT          384 a           81 c          148 g          342 t
ORIGIN      
        1 tatgtatata ctgaatttaa atacaagaga gactattgaa gattttaggg ataaatttta
       61 tgtagcagaa aattcttatc ttatattaag tgctccaaaa aatttaaaat tgcttaagga
      121 aactttggat atagatgaaa taacttttaa tgattgtctt aaatttgatg aaataaccaa
      181 acttgattta tttgataatt acgatttttt aagcctaaat acatttgaat taagagatgg
      241 agaagctgtt atagaagagg taaatatgta tttatcagat aattttatat tggttgtggt
      301 gaatgaagaa cactttttat ttgaatttgt aaaaaatatt attttgaaga actctcaact
      361 tgaaaaaaat ccagttatta atctatttaa gataaattac ttgattctta gagaagttat
      421 aaaaaatggt tttgagtctt tggaaaaagt tgaagagtta attcttcaaa tagaagatga
      481 aatgatggac aacattaata agaatcatgt atctagaata agtgatgtaa gaggattaac
      541 aaggattata gttaagaata ctagacctct tttgtatatt ggggatagaa tagttaagga
      601 aaatataaga tatttaaagt attcaaatgt aaaaaaatat aatttagaaa attttcaagg
      661 tatagatttt ggaattgaca aattatacaa tttcgcattg tcaacacgag agttagcaga
      721 taaattatta gatatttatt cttccagagt aggtgagaaa acaaataact taattacaaa
      781 attaaccttg ttaacagcca tatcagcacc acttacaatt ataactggta tatatggaat
      841 gaactttaga tatatgccag aattaaactg gatttatgga tatccagcaa ctttattttt
      901 tatgttatgt ataatttttg ttggaattat aatatttaaa ataaaaaaat tattg
//
LOCUS       DAEPXF010000382          943 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain HB27
            SAMN13376389-rid12479423.denovo.390, whole genome shotgun sequence.
ACCESSION   DAEPXF010000382 DAEPXF010000000
VERSION     DAEPXF010000382.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13376389
            Sequence Read Archive: SRR10518432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 943)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 943)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001069624.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001069624.1
            Genome Coverage       :: 315x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/15/2021 01:54:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,165
            CDSs (total)                      :: 4,076
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 89
            rRNAs                             :: 10, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 10 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 19 of 69
            Pseudo Genes (incomplete)         :: 44 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 10 of 69
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..943
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13376389-rid12479423.denovo.390"
                     /strain="HB27"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="China: Hebei"
                     /collection_date="2017"
     gene            136..276
                     /locus_tag="KRL67_003963"
     CDS             136..276
                     /locus_tag="KRL67_003963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH1524741.1"
                     /translation="MEKSNTTVTKKEKIEDIGMKLEHLLSEDITFYELLLEILYKEKE
                     EN"
     gene            complement(273..644)
                     /locus_tag="KRL67_003964"
     CDS             complement(273..644)
                     /locus_tag="KRL67_003964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBH1524742.1"
                     /translation="MESLGDRIANLRKELDINQKELATKVGITEASLSRYENNLREPK
                     SEIIVRLAKALETSTDYLLGVNDNTKISKEDKLIIENLSVSEKTKKLLEKIYSLEKED
                     REAIEKMIDNAYLKRFLKEEN"
BASE COUNT          359 a          102 c          113 g          369 t
ORIGIN      
        1 taattatcta gggaaatttt ggataatcaa aatatatttt ataaaatcaa aatacaatta
       61 cataaacttt attaaaaatt tgttgggaat ttctgactaa cattaagaac gtaagtttgt
      121 tgggggatgg tatatgtgga aaagtcaaac actactgtta caaaaaaaga aaaaattgaa
      181 gatataggaa tgaaattaga acatttattg agtgaagata ttacttttta tgaattatta
      241 cttgagattt tgtataaaga aaaagaagag aattaattct cttctttaag gaatcttttt
      301 aaataggcat tatcaatcat tttttctata gcttctctat cttctttttc aagagaatat
      361 attttttcta agagtttttt tgttttttca cttactgata agttctctat gattaattta
      421 tcttctttgc ttatttttgt attatcattt acgcctaata aataatctgt tgatgtttct
      481 aatgcttttg ctaatctaac tattatttca cttttaggtt ctcttaaatt attttcataa
      541 cgtgacaagc tagcctcagt tataccgact ttagtagcaa gttctttttg atttatatct
      601 aattcttttc gtagatttgc tattctatct cctaaacttt ccatatgtac atcactcctt
      661 accgaaatta taagttattt ttataaaaag taaaataaaa ttaccaaaaa tataaaaaaa
      721 aagggttaaa ataataaaat atattgaaaa ttaccatttg tatatgtata atataaatta
      781 tacaaatggt aatttgatga gaggtggtat gttgacataa gaaactaaat taattaataa
      841 atacaataga aacttttgaa tacccttaat tgagatataa acaatatgta taaaaatttt
      901 tgaaagtaaa attaccaaat gtataaaata aatcagtagc ata
//
LOCUS       DAEPXF010000383          911 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain HB27
            SAMN13376389-rid12479423.denovo.391, whole genome shotgun sequence.
ACCESSION   DAEPXF010000383 DAEPXF010000000
VERSION     DAEPXF010000383.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13376389
            Sequence Read Archive: SRR10518432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 911)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 911)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001069624.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001069624.1
            Genome Coverage       :: 315x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/15/2021 01:54:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,165
            CDSs (total)                      :: 4,076
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 89
            rRNAs                             :: 10, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 10 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 19 of 69
            Pseudo Genes (incomplete)         :: 44 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 10 of 69
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..911
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13376389-rid12479423.denovo.391"
                     /strain="HB27"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="China: Hebei"
                     /collection_date="2017"
     gene            108..671
                     /locus_tag="KRL67_003965"
     CDS             108..671
                     /locus_tag="KRL67_003965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH1524743.1"
                     /translation="MNKVYIVTTYTGTILSYLIRNISKKLYTHVSISLNENLKPMYSF
                     GRLNPRNPFIGGFVEENINQGLYAIRKNTVCRVYSLEVDNLQYENLYKNIKLISDYRE
                     DYYYDTMALIYLLMNIQRQSEYKYVCSNFVADMLEKSEIDILNKQAFEVTPNDFYNLS
                     GLTLEYEGLLSEYNSRQHSYVSKIANI"
BASE COUNT          369 a           88 c          131 g          323 t
ORIGIN      
        1 taccttaaaa tcaaaatttg taaaaccaca tttattatgt tagaatatct tatgatatta
       61 aattgtaagt gtataatatt atttagttac tagattgggg ggaaatgatg aataaagtct
      121 atattgtaac aacatataca ggaactatat tatcttactt aataaggaat ataagtaaaa
      181 aactgtatac acatgtgtca atatccttaa atgaaaatct aaaaccaatg tattcttttg
      241 gaagactgaa tccaaggaat ccatttattg gaggatttgt tgaagaaaat ataaatcaag
      301 gattatatgc tataagaaaa aacactgtgt gtagagtata ttcgttagaa gtagataatt
      361 tacaatatga aaatctatat aaaaatataa aattaattag tgattataga gaagattact
      421 attacgatac aatggcatta atatatttac taatgaatat tcagagacaa tctgaatata
      481 aatatgtatg ttcaaatttt gttgctgaca tgttagaaaa aagtgagata gacattttaa
      541 ataaacaagc atttgaagta acgccaaatg atttttataa tttatctggc ttaaccttag
      601 agtatgaagg tttattatca gaatataatt ctagacaaca ttcttatgta tcaaaaatcg
      661 caaacattta aaaacttgat ttagagtttt tcatataaaa aaatattgct agataattag
      721 gctgaagata gtagtttaac ttcgatagga ttaagaattt gtaaatctta gttagaatac
      781 tttcttcagt ttttaaattt atgatactat ttgtattttt ataatatttt tagatatata
      841 tattacagaa tagtacagaa atcccactcc tttaggtgtg agatggatag cacaaatagt
      901 gtataaaaag t
//
LOCUS       DAEPXF010000384          904 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain HB27
            SAMN13376389-rid12479423.denovo.392, whole genome shotgun sequence.
ACCESSION   DAEPXF010000384 DAEPXF010000000
VERSION     DAEPXF010000384.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13376389
            Sequence Read Archive: SRR10518432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 904)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 904)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001069624.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001069624.1
            Genome Coverage       :: 315x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/15/2021 01:54:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,165
            CDSs (total)                      :: 4,076
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 89
            rRNAs                             :: 10, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 10 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 19 of 69
            Pseudo Genes (incomplete)         :: 44 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 10 of 69
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..904
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13376389-rid12479423.denovo.392"
                     /strain="HB27"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="China: Hebei"
                     /collection_date="2017"
     gene            complement(<1..426)
                     /locus_tag="KRL67_003966"
     CDS             complement(<1..426)
                     /locus_tag="KRL67_003966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBH1524744.1"
                     /translation="MSGIISVMTQSLILSIMALGVYITYKILDFPDMSADGSYTMGAS
                     IVAFSLTNGISPVVATLMAILCGCVAGLVTGILHIKFKISNLLSGILVMGMLYSINLR
                     IMGKSNIPLFSFKHLFNGEISPIVLALAFVFICKVLLDLF"
BASE COUNT          371 a          132 c          113 g          288 t
ORIGIN      
        1 gaataagtct aaaaggactt tacagatgaa tacaaaagct aaggctaaaa ctataggaga
       61 tatttcccca ttgaataagt gtttaaagct aaatagagga atatttgatt ttcccattat
      121 tcttaaattg atagagtata gcattcccat aactaaaata ccagaaagta aattagatat
      181 cttaaactta atgtgaagaa tccctgtaac aagaccagca acacaaccac ataagatagc
      241 cattaaagtt gctactacag gagaaatacc attagtaaga gaaaatgcta cgatagaagc
      301 acccattgtg tagctaccat cagcagacat atctgggaaa tctaagattt tgtaagttat
      361 ataaaccccc agtgccataa ttgataaaat taaactttgc gtcattacag atataatacc
      421 tgacataaat aaaacacccc tttgaaaata ttttaaatgg ttttttaaat ttgattttaa
      481 aaaataattt tgatataaaa aaaccacaca gtatatataa ctgtatggca aaaatgcgaa
      541 taagccacac agtataatcc atgtggctct atattatgat gataaaataa aaactttaac
      601 aacaattact atagccaaca tagatacaat ccttaaaaat cataatccta aggggcttgt
      661 atctatgtta tgacaatttg tcttaactta ggtacaaacc ctaactaaat tggctactaa
      721 aaaatcgtac ttgtttgttt aataaagtct tcatatttac ctctcctttt aatttaataa
      781 ttttgaaaat tttgttttct gttttgtata gtaattagtt taatacagta aaaaataaaa
      841 gtcaactata aattttgaaa aattatcaaa ttatcagaaa attgagttta aataaaaaaa
      901 tttt
//
LOCUS       DAEPXF010000385          880 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain HB27
            SAMN13376389-rid12479423.denovo.393, whole genome shotgun sequence.
ACCESSION   DAEPXF010000385 DAEPXF010000000
VERSION     DAEPXF010000385.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13376389
            Sequence Read Archive: SRR10518432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 880)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 880)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001069624.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001069624.1
            Genome Coverage       :: 315x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/15/2021 01:54:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,165
            CDSs (total)                      :: 4,076
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 89
            rRNAs                             :: 10, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 10 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 19 of 69
            Pseudo Genes (incomplete)         :: 44 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 10 of 69
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..880
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13376389-rid12479423.denovo.393"
                     /strain="HB27"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="China: Hebei"
                     /collection_date="2017"
     gene            complement(155..640)
                     /locus_tag="KRL67_003967"
     CDS             complement(155..640)
                     /locus_tag="KRL67_003967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HBH1524745.1"
                     /translation="MISELMLKNFGKSSINFKTKIKLNLREKPDINSLKLKSIPEGKI
                     VKLKCVDGIWAEVESNYDKGWLLYKYLERLSNAKNNSNDTIKNRKKNYIGNIRTNGLS
                     LELRNDRTLESKVITTIPDGFKVEVCYSVGKWARVNINKNEKRYSGYVYNQYIEEHLS
                     L"
     regulatory      complement(723..816)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
BASE COUNT          290 a          133 c           69 g          388 t
ORIGIN      
        1 aattgaattt accatttata attactttat agtaaaaata aattagttaa tttattctct
       61 ttagttctgt tatattgttt ctacaaaaaa gaatcccatt aacgggattc ttttttgtga
      121 tttattaata tatttaaagc tcactagtat cgtattataa acttaaatgt tcttctatgt
      181 attgattata tacatatcca gaatatcgtt tttcattttt attgatatta actcttgccc
      241 attttccaac actataacat acttcaactt taaaaccatc agggattgta gttataactt
      301 tagattctaa tgttctatca tttctaagtt ctaaacttaa tccatttgtt cttatatttc
      361 ctatgtaatt cttttttcta ttttttattg tatcattact attgttttta gcattactta
      421 atctttctaa atatttataa agtaaccagc ctttgtcata attactctca acttctgccc
      481 atattccatc tacacatttt aacttaacaa ttttaccttc tggtatactt tttaatttca
      541 aactatttat gtctggtttt tctcttaaat tcaatttaat tttagtctta aaattgatgc
      601 tagatttgcc aaaatttttt aacattaatt cagatatcat cttaagccct cctcttgcca
      661 aatattaagt tattaaaata tataactcta aaaatacttt taacaaaaag aattattatc
      721 tttttggcaa ctggctgaca tgacctttat tacataaaga ttttagtccc tatagttttg
      781 cgtcattaga tttatctaat attgcctata tctgaataat aacataattt gaacaattaa
      841 aaaataatat tacctcaaat tttatattat ttaaatataa
//
LOCUS       DAEPXF010000386          879 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain HB27
            SAMN13376389-rid12479423.denovo.394, whole genome shotgun sequence.
ACCESSION   DAEPXF010000386 DAEPXF010000000
VERSION     DAEPXF010000386.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13376389
            Sequence Read Archive: SRR10518432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 879)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 879)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001069624.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001069624.1
            Genome Coverage       :: 315x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/15/2021 01:54:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,165
            CDSs (total)                      :: 4,076
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 89
            rRNAs                             :: 10, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 10 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 19 of 69
            Pseudo Genes (incomplete)         :: 44 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 10 of 69
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..879
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13376389-rid12479423.denovo.394"
                     /strain="HB27"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="China: Hebei"
                     /collection_date="2017"
     gene            249..638
                     /locus_tag="KRL67_003968"
     CDS             249..638
                     /locus_tag="KRL67_003968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HBH1524746.1"
                     /translation="MKINKLPHAELKLMKYIWGVDDVLASRDIIEDMKLKYDWKKSTT
                     LTFLKNLVDKGFLTTDKVDRCTHYTIAIKEKDYLKVETRSFFSFMHNNSFKSFISALH
                     DDEVLDSKSLDKLEEYFKNLKEEDIDD"
BASE COUNT          371 a           79 c          129 g          300 t
ORIGIN      
        1 tataatacat attataataa taaaacgaac taaaattatc acactaaagc ataaaacata
       61 ctctaatcat gttagataaa tttttgaata tataatccca aagtactctc taaagtagag
      121 tacttctttt ttattaatta tttttatata gaataaattg gaatttatat ttatcccaat
      181 ctaattgatt acatacactt attgttataa agtttagtta tatataaatt ggtaagagtt
      241 gtaaactaat gaagattaat aaattaccac atgcagaact aaaactaatg aagtatattt
      301 ggggagtaga tgatgtatta gcctcaagag atatcataga agacatgaag ttgaaatacg
      361 attggaaaaa gagcacaaca ttgacatttt taaaaaattt agtagataag ggctttttaa
      421 ctactgataa ggtagataga tgtacgcatt atactatagc tataaaagag aaagattact
      481 taaaagttga gacaaggagt ttttttagtt ttatgcataa caattcattt aagagtttta
      541 tttcagcact acatgatgat gaagttttag atagtaagag cttggacaaa ttagaagaat
      601 actttaaaaa tttgaaagaa gaagatattg atgactaata aatctatcag tatcttttta
      661 gtttgagaca attgagagta gcgttttgat ttaatgttag tgttatgtga tggtagaaca
      721 ttatataaag aaaagtgctt atttccttag tatctgatag taaaaaaaat atagaaaata
      781 ataccaatta aaatttaaag aaagatatga aatactacaa tagcttaaat catgtaaata
      841 tgataaagaa attgaaatta taaaaggaat ataatttta
//
LOCUS       DAEPXF010000387          816 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain HB27
            SAMN13376389-rid12479423.denovo.395, whole genome shotgun sequence.
ACCESSION   DAEPXF010000387 DAEPXF010000000
VERSION     DAEPXF010000387.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13376389
            Sequence Read Archive: SRR10518432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 816)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 816)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001069624.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001069624.1
            Genome Coverage       :: 315x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/15/2021 01:54:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,165
            CDSs (total)                      :: 4,076
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 89
            rRNAs                             :: 10, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 10 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 19 of 69
            Pseudo Genes (incomplete)         :: 44 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 10 of 69
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..816
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13376389-rid12479423.denovo.395"
                     /strain="HB27"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="China: Hebei"
                     /collection_date="2017"
     gene            186..692
                     /locus_tag="KRL67_003969"
     CDS             186..692
                     /locus_tag="KRL67_003969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase"
                     /protein_id="HBH1524747.1"
                     /translation="MGFREVKIEELQFNPFTKIGKEWLLITAGDSEKFNTMTASWGGV
                     GVYWGKNVVTTYIRPQRYTKEFVDSNDTFTVAFFDETYREALNICGTVSGRDINKIEK
                     AGLTPYFVDDTVAFEEANMIIVCKKLYHGNMPPENFDAKENDKKWYPEKDYHTMYISE
                     IIKILVKE"
BASE COUNT          332 a           80 c          134 g          270 t
ORIGIN      
        1 atattttatc tatattttac acttataaaa tataaaaact acttattgcg aaagtaattg
       61 atttgttgta ttataattta catgaattat gtaggacaat caaaagtcag tattataaga
      121 gtacatatat tcaaattttt aacaattaat ttatataatt aaaaaatgaa gggtaggatg
      181 aagtcatggg ttttagagaa gtaaaaattg aggaactaca gtttaatcca tttactaaga
      241 ttggtaaaga atggttactt ataacagctg gtgattcgga aaagtttaac acaatgactg
      301 ctagctgggg tggtgttgga gtatattggg gtaaaaatgt tgttacaact tacataagac
      361 cacagagata tacaaaagaa tttgtagata gtaatgatac atttacagtt gccttctttg
      421 atgaaacgta tagagaagct ttaaatattt gtggtactgt ttccggtaga gatataaata
      481 aaatagaaaa agcaggactt acaccatatt ttgtggatga tacggtagct tttgaagaag
      541 ctaatatgat tatagtatgt aaaaagttat atcatggtaa tatgccacca gaaaactttg
      601 atgctaaaga aaatgataaa aaatggtatc cagagaaaga ctatcatact atgtacattt
      661 cagaaattat aaaaattctt gtaaaagaat agttataata aaaaatatct gaaaagaact
      721 tattataagt aaatgactgt gctttcttat ctgatatcta agtagatgct actaactaaa
      781 taaatattaa ctttaaataa atactattat aaaaaa
//
LOCUS       DAEPXF010000388          808 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain HB27
            SAMN13376389-rid12479423.denovo.396, whole genome shotgun sequence.
ACCESSION   DAEPXF010000388 DAEPXF010000000
VERSION     DAEPXF010000388.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13376389
            Sequence Read Archive: SRR10518432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 808)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 808)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001069624.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001069624.1
            Genome Coverage       :: 315x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/15/2021 01:54:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,165
            CDSs (total)                      :: 4,076
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 89
            rRNAs                             :: 10, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 10 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 19 of 69
            Pseudo Genes (incomplete)         :: 44 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 10 of 69
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..808
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13376389-rid12479423.denovo.396"
                     /strain="HB27"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="China: Hebei"
                     /collection_date="2017"
     gene            434..>808
                     /locus_tag="KRL67_003970"
     CDS             434..>808
                     /locus_tag="KRL67_003970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBH1524748.1"
                     /translation="MQIDVLIIDGDKNSSQELKNFLEGKGINVYLAYNCKDAIIKIFS
                     KKFDLIFLEIILVDGDGWDFCKKIRNITTCPIIHITYINENQSILNALNSGGDDYLIK
                     PLNLEILYAKVNAILRRRDLFLN"
BASE COUNT          340 a           53 c          118 g          297 t
ORIGIN      
        1 taataatcga aattttatac tttggttaaa ataattttat atatagtgcc taatgtataa
       61 ccgaatgcgc gtagacaata atgtggatat tatcaaattt atctttatat taaattgtta
      121 aagacataat tatgagaata aaaaatatag ctttgaatta tataattgta tatgcttatc
      181 tttatacagt aaataaaaaa tcaaatgaaa tactaaataa aatgtatatc ttaaaataac
      241 ttactagata tacaagcatg ttatgtgaat tttgaagtgt gcagtttaga taataaatat
      301 atacaaataa taaagaaaat ggatttatag gatttaaaat ttcttataag ggtaaaaaat
      361 gttattagaa aatgtactaa aaatattttt ccaattcttt atagaaaaat aatgatattt
      421 aggagagatt gtaatgcaaa ttgatgtatt gataattgat ggagataaga atagttctca
      481 agagctgaaa aattttttag aaggaaaagg gataaatgtc tatttagcat ataattgtaa
      541 agatgctatt ataaaaatat ttagcaagaa atttgactta atatttttag agataatttt
      601 ggttgatggt gatggttggg atttttgtaa aaaaattaga aatataacta cgtgcccaat
      661 tattcatata acttatataa atgaaaatca aagtatttta aatgctttaa attctggtgg
      721 agatgattat ttaataaagc ctttaaattt agaaatatta tatgcgaaag tgaatgctat
      781 tttaagaaga agagatttat ttttaaat
//
LOCUS       DAEPXF010000389          792 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain HB27
            SAMN13376389-rid12479423.denovo.397, whole genome shotgun sequence.
ACCESSION   DAEPXF010000389 DAEPXF010000000
VERSION     DAEPXF010000389.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13376389
            Sequence Read Archive: SRR10518432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 792)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 792)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001069624.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001069624.1
            Genome Coverage       :: 315x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/15/2021 01:54:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,165
            CDSs (total)                      :: 4,076
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 89
            rRNAs                             :: 10, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 10 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 19 of 69
            Pseudo Genes (incomplete)         :: 44 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 10 of 69
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..792
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13376389-rid12479423.denovo.397"
                     /strain="HB27"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="China: Hebei"
                     /collection_date="2017"
     gene            83..781
                     /locus_tag="KRL67_003971"
     CDS             83..781
                     /locus_tag="KRL67_003971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBH1524749.1"
                     /translation="MEKKYFKIHGVPSILWGNPSSKLYIYIHGQGGNKEEAEAFANIA
                     VPHECQVLSVDLPEHGERKGEKNSFDPWHVVPELVSIMEYAKCHWNQISLYANSIGAW
                     FSMLSFEKENLRECIFVSPILDMQKLISNMMLWANVSEEQLKHELIIPTSFGHTLSWE
                     YLQYVKQHPIIQWNVPTKILYGKHDELTEIGVVEKFVEKFNCNLTIMENGKHWFNTPQ
                     ELNSLYEWLNTLVK"
BASE COUNT          296 a          105 c          134 g          257 t
ORIGIN      
        1 tattaaatta tatattacaa cattatttta tagtttgatt aatttaacaa taactaatat
       61 atcaaactga gaggagattt ttatggaaaa aaaatatttc aaaattcatg gtgttccttc
      121 aatcctttgg ggaaatcctt caagtaaatt atatatttac atacatggac aaggtggcaa
      181 taaagaggag gctgaagctt ttgctaatat tgcagtccca catgagtgtc aagttttaag
      241 tgttgattta cctgagcatg gagagcgtaa aggagaaaaa aattcatttg acccatggca
      301 tgtggttcca gaactagtat ctattatgga atatgcaaag tgccactgga atcaaatttc
      361 gttatatgca aatagcattg gagcatggtt tagtatgtta agctttgaaa aagagaactt
      421 aagagagtgt atttttgtat ctccaattct agatatgcaa aagcttattt caaatatgat
      481 gctttgggca aatgtatcag aagaacagct taaacatgaa ctaattatcc caacttcatt
      541 tgggcatact ctttcatggg aatatctaca atatgtaaaa caacatccta tcatccaatg
      601 gaatgtacca acaaaaatat tatatggtaa acatgatgag cttactgaaa tcggtgttgt
      661 agagaaattt gtagaaaagt ttaattgcaa ccttactatt atggaaaatg gaaaacattg
      721 gtttaacaca ccccaagaat taaattcatt atatgaatgg ttaaatactt tagttaaata
      781 aaaaataatt at
//
LOCUS       DAEPXF010000390          769 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain HB27
            SAMN13376389-rid12479423.denovo.398, whole genome shotgun sequence.
ACCESSION   DAEPXF010000390 DAEPXF010000000
VERSION     DAEPXF010000390.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13376389
            Sequence Read Archive: SRR10518432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 769)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 769)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001069624.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001069624.1
            Genome Coverage       :: 315x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/15/2021 01:54:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,165
            CDSs (total)                      :: 4,076
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 89
            rRNAs                             :: 10, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 10 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 19 of 69
            Pseudo Genes (incomplete)         :: 44 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 10 of 69
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..769
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13376389-rid12479423.denovo.398"
                     /strain="HB27"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="China: Hebei"
                     /collection_date="2017"
     gene            13..480
                     /locus_tag="KRL67_003972"
     CDS             13..480
                     /locus_tag="KRL67_003972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2975 domain-containing protein"
                     /protein_id="HBH1524750.1"
                     /translation="MITKFFSNILSVFLYFLLSLLGVAGIIFLCMIPMFLYDFSSDKV
                     IALIARILYFSDYFIVILFLIFIINSTKYSPFILENVKRFKVMGCCLLINTIIECVIG
                     YNSNTSLIMIFGDNSGGISPLMVISFIFALMCFVIAETFDKAIKIKEDNDLTI"
     gene            491..706
                     /locus_tag="KRL67_003973"
     CDS             491..706
                     /locus_tag="KRL67_003973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBH1524751.1"
                     /translation="MAIVVNLDVMMAKRKISLKDLAEKIDITNANLSILKTGKAKAIR
                     FTTLNEICKALDCQPGDILEYVEDEND"
BASE COUNT          278 a           81 c          115 g          295 t
ORIGIN      
        1 agatgatatt ttatgattac aaaatttttt agtaatatat tatcagtatt tctatatttt
       61 ctattatcac ttttaggagt tgctggtata atattcttat gtatgatacc tatgttttta
      121 tatgatttta gttctgacaa agtaatcgct ttaattgcaa gaatactata tttttctgac
      181 tactttatag tcatcttatt cttgatattt ataattaata gtactaagta ttccccattc
      241 atacttgaaa atgtaaaaag gtttaaagta atgggatgtt gtttattgat aaacactata
      301 attgaatgtg taattggtta taactcaaat actagtctta taatgatatt tggagataat
      361 agtggcggta tctcaccact tatggttatt tcttttatat tcgcactaat gtgctttgta
      421 attgcagaaa catttgataa agctataaaa ataaaagaag ataatgattt aacgatatag
      481 aggtgatgtt atggcaatag tagttaacct agatgtaatg atggcaaaaa ggaaaatatc
      541 acttaaagat ttggcagaaa aaatagatat tacaaatgca aatttatcaa ttttaaaaac
      601 tggaaaagca aaagctatta gatttaccac tctaaacgaa atttgcaaag ctttagactg
      661 tcagccaggt gatatacttg aatatgtaga agatgaaaat gattagaaaa gagtgtttag
      721 agacactctt ttttattata ctatatgatt tttattcttt tactatatt
//
LOCUS       DAEPXF010000391          751 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain HB27
            SAMN13376389-rid12479423.denovo.399, whole genome shotgun sequence.
ACCESSION   DAEPXF010000391 DAEPXF010000000
VERSION     DAEPXF010000391.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13376389
            Sequence Read Archive: SRR10518432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 751)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 751)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001069624.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001069624.1
            Genome Coverage       :: 315x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/15/2021 01:54:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,165
            CDSs (total)                      :: 4,076
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 89
            rRNAs                             :: 10, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 10 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 19 of 69
            Pseudo Genes (incomplete)         :: 44 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 10 of 69
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..751
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13376389-rid12479423.denovo.399"
                     /strain="HB27"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="China: Hebei"
                     /collection_date="2017"
     gene            245..613
                     /locus_tag="KRL67_003974"
     CDS             245..613
                     /locus_tag="KRL67_003974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C-GCAxxG-C-C family protein"
                     /protein_id="HBH1524752.1"
                     /translation="MTRPSIYHSQGYTCAEALIKSYNEEHNTDIPISIGSGMGVGMNV
                     GSVCGAVNAAVVIIGYLNGRNSNLDENIARKYSRELLNRVRNKYSSEICVSLKKNKVT
                     CEEIINFTYDALNDMLKNQK"
BASE COUNT          327 a           60 c          116 g          248 t
ORIGIN      
        1 attgtgtgac taagtcacag aaaatagagt taatttaata tataataaaa gaatatcaag
       61 taaagcttta aattaaagta actttataaa taaaaagtaa tataaattga taaaattata
      121 aatttattta tagtgaatat aggttggatt ttatagatac aaaaatagta tatatgatat
      181 gtagaccatt agcatgttta aagtagcaaa acatataaaa taaaatttga aaggaagtat
      241 aaaaatgaca agaccatcaa tatatcatag ccaaggatac acttgtgcag aggcattaat
      301 aaaatcttat aatgaggaac acaacacaga tataccaatc tcaataggaa gtggtatggg
      361 ggttggaatg aatgttggaa gtgtttgtgg agctgtaaat gctgctgttg ttataatagg
      421 gtatttaaat gggcgaaata gtaatttaga tgaaaatata gcaaggaagt attcaagaga
      481 actattgaat agagtaagaa ataaatacag ctcagaaata tgtgtatctc ttaaaaaaaa
      541 taaagtaact tgtgaagaaa taataaattt cacatatgat gctctaaatg atatgctaaa
      601 aaatcaaaaa taattataag aaacaagtat tttacattcg ataaaatact tgttttttta
      661 tgatattttt aataggtatt agtttattat aatttttaaa tcaattttat tatattttaa
      721 ttgaagtaat ctactttgaa aattactaac t
//
LOCUS       DAEPXF010000392          710 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain HB27
            SAMN13376389-rid12479423.denovo.400, whole genome shotgun sequence.
ACCESSION   DAEPXF010000392 DAEPXF010000000
VERSION     DAEPXF010000392.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13376389
            Sequence Read Archive: SRR10518432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 710)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 710)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001069624.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001069624.1
            Genome Coverage       :: 315x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/15/2021 01:54:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,165
            CDSs (total)                      :: 4,076
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 89
            rRNAs                             :: 10, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 10 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 19 of 69
            Pseudo Genes (incomplete)         :: 44 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 10 of 69
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..710
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13376389-rid12479423.denovo.400"
                     /strain="HB27"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="China: Hebei"
                     /collection_date="2017"
     gene            57..599
                     /locus_tag="KRL67_003975"
     CDS             57..599
                     /locus_tag="KRL67_003975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HBH1524753.1"
                     /translation="MKICITVGHSILKGGKSTGVNGIVDEYNYNKKLAPMLAEMLISQ
                     GNTVDVIICPERHFMSEREEFFYRVPKVNSGKYDLLVELHLNKADGTQCGTEVLYYGK
                     EGLEYAKRVSNRLGELFENRGAKKRENLYILRNTNPVAIQIESFFCDNVNDCNKANES
                     GYDYIARLITEGILNKDINM"
BASE COUNT          301 a           58 c          131 g          220 t
ORIGIN      
        1 ttaaaattat attactatta tataaaaatt ggcttaaaag aaggtgaaaa aagtaaatga
       61 aaatatgcat aacagtaggt catagcatac taaaaggtgg taaaagtaca ggtgtaaatg
      121 gtatagttga tgaatacaat tataacaaaa aattagctcc tatgttagct gagatgctta
      181 taagtcaagg taatacagta gatgtaataa tttgtccaga aagacacttt atgtcagaaa
      241 gagaagaatt tttctataga gttccaaaag taaacagtgg taaatacgat ttattagtag
      301 agctacattt aaataaagct gatggaacac aatgtggtac agaagtgctt tactatggca
      361 aggaaggact agaatatgct aaaagagtat ctaatagact tggagaatta tttgaaaata
      421 gaggagctaa aaaaagagaa aatttatata tattgagaaa tacaaatcca gttgcaatac
      481 agattgagag ttttttctgt gataatgtga atgattgtaa caaagcaaat gaatcaggtt
      541 atgattatat agcacgattg attacagaag gtatattgaa taaagatata aatatgtgag
      601 attatggtga tttaggtatt gattaatacc ttttaatatt agatataaat aaaaatacaa
      661 tatattagta taatttaaac ttgcaaaaga tagattagtt tgattattga
//
LOCUS       DAEPXF010000393          694 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain HB27
            SAMN13376389-rid12479423.denovo.401, whole genome shotgun sequence.
ACCESSION   DAEPXF010000393 DAEPXF010000000
VERSION     DAEPXF010000393.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13376389
            Sequence Read Archive: SRR10518432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 694)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 694)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001069624.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001069624.1
            Genome Coverage       :: 315x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/15/2021 01:54:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,165
            CDSs (total)                      :: 4,076
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 89
            rRNAs                             :: 10, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 10 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 19 of 69
            Pseudo Genes (incomplete)         :: 44 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 10 of 69
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..694
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13376389-rid12479423.denovo.401"
                     /strain="HB27"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="China: Hebei"
                     /collection_date="2017"
     gene            90..>694
                     /locus_tag="KRL67_003976"
     CDS             90..>694
                     /locus_tag="KRL67_003976"
                     /inference="COORDINATES: protein motif:HMM:NF033516.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HBH1524754.1"
                     /translation="MLKKVKYPIGQVKVSDKFKTIKLLSRKYSISSMCKLIGVSRSGY
                     YKWLSYSKKSSDRGIKDRIIKDYIIEIHKKYRGTYGRKRICTYLNKILDTPINHKRVY
                     RLMKELGIKSIIRKKVYRRKFKSYEIYDNILNREFRANQPLEKICMDITYIPIGKKFL
                     YMNVAKDLFNGEIVAYEISTKMDTKLVNNTVNQLINMNLAK"
BASE COUNT          302 a           60 c          115 g          217 t
ORIGIN      
        1 ggcagattta aaaatattag tctagaaaat gataatagaa aatttaaatc tgttgaagaa
       61 gaattgaagt acttacgcat ggagaatgaa ttcttaaaaa agttaagtac cctattggac
      121 aagtcaaagt aagcgataaa tttaaaacaa ttaaacttct gtctaggaag tatagtattt
      181 ctagtatgtg taagttgata ggtgtgtcaa gaagcggtta ctataaatgg ctaagttatt
      241 ctaaaaaatc ttccgatagg ggtatcaaag atagaattat taaggattat ataattgaaa
      301 tacataaaaa atatagagga acttatggta gaaaaagaat ttgtacttat ttaaataaaa
      361 tacttgatac ccctattaat cataagagag tatataggtt gatgaaagaa ctaggtatta
      421 aatctattat tagaaagaaa gtatatagac gtaagtttaa atcatatgag atatatgata
      481 atatattaaa tcgtgaattt agagctaatc aaccattaga aaaaatttgt atggatataa
      541 catacatacc tatcggtaaa aaattcttat atatgaatgt agccaaagat ttatttaatg
      601 gtgaaatagt ggcatatgag ataagtacaa aaatggatac taaattagtt aataatacag
      661 taaatcaact aataaatatg aatttagcta aaga
//
LOCUS       DAEPXF010000394          689 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain HB27
            SAMN13376389-rid12479423.denovo.402, whole genome shotgun sequence.
ACCESSION   DAEPXF010000394 DAEPXF010000000
VERSION     DAEPXF010000394.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13376389
            Sequence Read Archive: SRR10518432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 689)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 689)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001069624.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001069624.1
            Genome Coverage       :: 315x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/15/2021 01:54:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,165
            CDSs (total)                      :: 4,076
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 89
            rRNAs                             :: 10, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 10 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 19 of 69
            Pseudo Genes (incomplete)         :: 44 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 10 of 69
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..689
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13376389-rid12479423.denovo.402"
                     /strain="HB27"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="China: Hebei"
                     /collection_date="2017"
     gene            <1..270
                     /locus_tag="KRL67_003977"
     CDS             <1..270
                     /locus_tag="KRL67_003977"
                     /inference="COORDINATES: protein motif:HMM:NF025063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HBH1524755.1"
                     /translation="GSQYTSRSYSKRLKDNGIIQSMSRRGNCWDNAPIESFFSHFKSE
                     LIYLIDTTDPKEMISLINDYIYFYNNERIQLKNGMSPIEYRTHSE"
     gene            complement(466..666)
                     /locus_tag="KRL67_003978"
     CDS             complement(466..666)
                     /locus_tag="KRL67_003978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH1524756.1"
                     /translation="MKKLKIISIFSLIISVILTIGGIGIVTYYVDNLFIRGLSVFVLI
                     MSSSFVSTTVRLIFEGSKRYKF"
BASE COUNT          264 a           99 c           84 g          242 t
ORIGIN      
        1 ggttcacaat atacaagtag atcttactct aaaaggctta aggataatgg tattatacaa
       61 tcaatgtccc gaaggggaaa ctgttgggat aatgctccta tagaaagttt tttcagtcat
      121 tttaaatctg aattaatata tttaattgat actacagatc caaaggaaat gattagtcta
      181 ataaatgatt atatttattt ttataataac gaaagaatac aattaaaaaa cggaatgagt
      241 ccgattgaat atcgaactca ttccgagtaa agatagcctt ttttaagtct gtctacttga
      301 cagggacata tccaaaatag agataagcct ctttttcatt gttgaaatga tgaaaatata
      361 tttaatttca tacaaaaaaa tgcctcatgg actaaaaagt ctattttgag ctaatctctg
      421 taatttttgg tatattaaaa ttatttactt aatccaattt ttaaattaga atttatatcg
      481 ttttgaccct tcaaatatca atctaactgt tgttgataca aaagaagagg acataattaa
      541 aacaaatacc gatagtcctc taataaacaa attatctaca taataagtta ctattccaat
      601 acctccaatc gttaaaatta cacttataat caaagaaaaa atgctaatta tttttaattt
      661 tttcatgctt atcctccttc ttataaact
//
LOCUS       DAEPXF010000395          673 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain HB27
            SAMN13376389-rid12479423.denovo.403, whole genome shotgun sequence.
ACCESSION   DAEPXF010000395 DAEPXF010000000
VERSION     DAEPXF010000395.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13376389
            Sequence Read Archive: SRR10518432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 673)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 673)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001069624.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001069624.1
            Genome Coverage       :: 315x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/15/2021 01:54:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,165
            CDSs (total)                      :: 4,076
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 89
            rRNAs                             :: 10, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 10 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 19 of 69
            Pseudo Genes (incomplete)         :: 44 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 10 of 69
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..673
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13376389-rid12479423.denovo.403"
                     /strain="HB27"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="China: Hebei"
                     /collection_date="2017"
     gene            complement(<1..>673)
                     /locus_tag="KRL67_003979"
     rRNA            complement(<1..>673)
                     /locus_tag="KRL67_003979"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          138 a          192 c          142 g          201 t
ORIGIN      
        1 cagtctgact cccaagtata agatgacggc attcggagtt tgatagtctt cggtaggtgc
       61 aataccccct aggacattca gtgctctacc tccgtatctc tcaccttgag gctagcccta
      121 aagctatttc ggggagaacc agctatctcc gggctcgatt ggaatttcac cgctacccac
      181 aagtcatccc cgagcttttc aacgctcgtg ggttcggacc tccacgaaat tttactttcg
      241 cttcatcctg ctcatgggta ggtcgcccgg tttcgggtct acgtcaagta actgaacgct
      301 cagttaaaac tcgctttcgc tacggctccg caccttaagt gcttaacctt gctacttaac
      361 gtaactcgtt ggcccgttct acaaaaagta cgcggtcaca caagaaatgt gcttccacag
      421 cttgtaagtg cagggtttca ggttctattt cactcccctc ccggggttct tttcaccttt
      481 ccctcacggt actatacgct atcggtcact aggtagtatt tagccttgga gggtggtccc
      541 tcctgcttcc cacagggttt cacgtgtccc gtggtactct ggatcatatc tgaagtcttc
      601 tcgtttaaca tacaggacta ttaccttctg tggtgggtct ttccaaacct cttcaattac
      661 gatacctctt cgt
//
LOCUS       DAEPXF010000396          672 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain HB27
            SAMN13376389-rid12479423.denovo.404, whole genome shotgun sequence.
ACCESSION   DAEPXF010000396 DAEPXF010000000
VERSION     DAEPXF010000396.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13376389
            Sequence Read Archive: SRR10518432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 672)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 672)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001069624.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001069624.1
            Genome Coverage       :: 315x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/15/2021 01:54:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,165
            CDSs (total)                      :: 4,076
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 89
            rRNAs                             :: 10, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 10 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 19 of 69
            Pseudo Genes (incomplete)         :: 44 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 10 of 69
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..672
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13376389-rid12479423.denovo.404"
                     /strain="HB27"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="China: Hebei"
                     /collection_date="2017"
     gene            144..614
                     /locus_tag="KRL67_003980"
     CDS             144..614
                     /locus_tag="KRL67_003980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase/bleomycin resistance/dioxygenase
                     family protein"
                     /protein_id="HBH1524757.1"
                     /translation="MKYQVSLLAVKDIEVSKKFYKELFEQEIELDLGKNVTFKGGFAI
                     QEDFAWLTGINPDTIIQKSNNMELYFEVDDFDEFIEKLNYYKDVEFVHKPLKHEWQQR
                     VVRIYDPDKHIIEIGESMEVIARRYLSDGYSVEETSKIIQHPIEFVKQCENSKE"
BASE COUNT          298 a           56 c          123 g          195 t
ORIGIN      
        1 gaagagatag aaacaaaagt gttaatagaa gatactaaaa aagtaaaaat aaaggattaa
       61 taaaagatac tgaagagata gagacaaaaa tgttaataga agatactgaa aaaaatagaa
      121 ataaaaaatt aggaggaaaa tttatgaaat atcaggtttc attattagca gtgaaggata
      181 ttgaagtatc aaaaaaattt tacaaagaat tatttgaaca agaaattgaa ttggacttag
      241 gtaaaaatgt aacatttaaa ggaggatttg ctatacaaga agacttcgca tggcttacag
      301 ggattaatcc agatacaatt atacaaaaat caaataatat ggaactttat tttgaggttg
      361 atgattttga cgaatttatt gaaaaactca attattataa agatgtagag tttgtccata
      421 agccactaaa acatgaatgg caacaaagag tagtgcgtat ttatgaccca gataagcata
      481 ttattgaaat aggagagtct atggaagtta tagctagaag gtatttatct gatggatact
      541 ctgtagaaga aacttctaaa ataattcaac atccaattga atttgtaaag cagtgtgaaa
      601 atagtaagga atgattgttt aaattataat tgtttagtag aaattaattg tagtggaact
      661 aattcaagat aa
//
LOCUS       DAEPXF010000397          664 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain HB27
            SAMN13376389-rid12479423.denovo.405, whole genome shotgun sequence.
ACCESSION   DAEPXF010000397 DAEPXF010000000
VERSION     DAEPXF010000397.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13376389
            Sequence Read Archive: SRR10518432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 664)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 664)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001069624.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001069624.1
            Genome Coverage       :: 315x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/15/2021 01:54:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,165
            CDSs (total)                      :: 4,076
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 89
            rRNAs                             :: 10, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 10 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 19 of 69
            Pseudo Genes (incomplete)         :: 44 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 10 of 69
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..664
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13376389-rid12479423.denovo.405"
                     /strain="HB27"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="China: Hebei"
                     /collection_date="2017"
     gene            complement(<1..>664)
                     /locus_tag="KRL67_003981"
     rRNA            complement(<1..>664)
                     /locus_tag="KRL67_003981"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          152 a          191 c          151 g          170 t
ORIGIN      
        1 gatccagccg caccttccga tacggctacc ttgttacgac ttcaccccag ttattgattc
       61 caccttcgac gacttcttcc aaaaggttag ataatcggct tcgggcgtct ccaactcccg
      121 tggtgtgacg ggcggtgtgt acaagacccg ggaacgcatt caccgcagca ttctgatctg
      181 cgattactag taactccagc ttcatgtagg cgagtttcag cctacaatcc gaactgagag
      241 tagctttaag ggattagctc caccttacgg cttggcaacc ctctgtacta cccattgtag
      301 cacgtgtgta gccctaagca taaggggcat gatgatttga cgtcatcccc accttcctcc
      361 aggttatccc tggcagtctc tctagagtgc ccaacttaat gatggcaact aaagacaagg
      421 gttgcgctcg ttgcgggact taacccaaca tctcacgaca cgagctgacg acaaccatgc
      481 accacctgtc accaatgtcc ccgaagggaa ctctccgatt aaggagatgt cattgggatg
      541 tcaagcttag gtaaggttct tcgcgttgct tcgaattaaa ccacatgctc cgctacttgt
      601 gcgggtcccc gtcaattcct ttgagtttca ctcttgcgag cgtacttccc aggcggagta
      661 ctta
//
LOCUS       DAEPXF010000398          664 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain HB27
            SAMN13376389-rid12479423.denovo.406, whole genome shotgun sequence.
ACCESSION   DAEPXF010000398 DAEPXF010000000
VERSION     DAEPXF010000398.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13376389
            Sequence Read Archive: SRR10518432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 664)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 664)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001069624.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001069624.1
            Genome Coverage       :: 315x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/15/2021 01:54:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,165
            CDSs (total)                      :: 4,076
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 89
            rRNAs                             :: 10, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 10 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 19 of 69
            Pseudo Genes (incomplete)         :: 44 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 10 of 69
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..664
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13376389-rid12479423.denovo.406"
                     /strain="HB27"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="China: Hebei"
                     /collection_date="2017"
     gene            74..>664
                     /locus_tag="KRL67_003982"
     CDS             74..>664
                     /locus_tag="KRL67_003982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HBH1524758.1"
                     /translation="MLNETRKKQLTEVCQDLIRNASYSGQEENVVKAIEENFALLGFD
                     SWSRDRYGNIVGCIKGNKPGKKILFDGHIDTVPVPDASKWSVPPFEARIVDGKIYGRG
                     TSDMKGQVSAMMAAVSYFAEDTNKDFEGELYVAGVVHEEIFEGVSAREISKAVQPDYV
                     VIGESSELNLKIGQRGRGEIVVETFGKPAHSANPEKG"
BASE COUNT          268 a           70 c          147 g          179 t
ORIGIN      
        1 gctaataaat taaaaaagtg aaatatcaaa ataaaacgtc aacaaattaa aaattatatg
       61 ggggaatatc aaaatgttaa atgaaacaag aaaaaaacaa ttaactgaag tatgccaaga
      121 cttaataaga aatgctagtt attcaggtca agaagaaaac gtagtaaaag ctatagaaga
      181 gaattttgct ttactaggtt ttgatagttg gtcaagagat agatatggaa atatagtagg
      241 atgtataaaa ggaaataaac caggtaaaaa aatattattt gacgggcata tagatacagt
      301 tccagttcca gatgcaagta aatggagtgt tcctcctttt gaagcgagaa tagtagatgg
      361 gaaaatatat ggtagaggaa cttctgatat gaaggggcaa gtaagtgcta tgatggctgc
      421 tgtttcatac tttgcagaag atactaacaa agattttgaa ggtgaattgt acgtagcagg
      481 tgttgttcat gaagaaatat ttgaaggagt ttcagctaga gagataagta aggctgttca
      541 acctgattat gttgtaatag gtgaatcttc agaattaaac ttaaagatag gccaaagagg
      601 tagaggagaa atagtagtag aaacttttgg taaaccagct cactctgcta atccagaaaa
      661 aggt
//
LOCUS       DAEPXF010000399          651 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain HB27
            SAMN13376389-rid12479423.denovo.407, whole genome shotgun sequence.
ACCESSION   DAEPXF010000399 DAEPXF010000000
VERSION     DAEPXF010000399.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13376389
            Sequence Read Archive: SRR10518432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 651)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 651)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001069624.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001069624.1
            Genome Coverage       :: 315x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/15/2021 01:54:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,165
            CDSs (total)                      :: 4,076
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 89
            rRNAs                             :: 10, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 10 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 19 of 69
            Pseudo Genes (incomplete)         :: 44 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 10 of 69
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..651
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13376389-rid12479423.denovo.407"
                     /strain="HB27"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="China: Hebei"
                     /collection_date="2017"
     gene            161..445
                     /locus_tag="KRL67_003983"
     CDS             161..445
                     /locus_tag="KRL67_003983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH1524759.1"
                     /translation="MLVYNKSFYPNDIFPRLDFSKIKKQLKLIDNDLSDFGSICIIEK
                     EHYTISVNSIGEINVYYDLEYENKVYRIVYEIEKLFKSQVGRFSISTYRN"
BASE COUNT          280 a           47 c           93 g          231 t
ORIGIN      
        1 ttttactaat atacatttaa aaattacaat ttaaaacatt aaagaataaa tttgtatttt
       61 gttttatata aagtaatttg aagaatatgg aaaaacatga ttgctacatt attaattagg
      121 gtataaaatg gtataaatag aatgataagg ggtggatgaa atgcttgtat ataataaaag
      181 tttttatcct aatgacatat ttccaagatt agatttttca aaaataaaaa aacagttaaa
      241 actgatagat aatgacctgt cagattttgg aagcatatgt ataatagaaa aagaacatta
      301 tacaataagt gtaaacagta taggtgaaat aaatgtgtat tatgatttag agtacgaaaa
      361 taaggtgtat agaatagttt atgagattga aaagttattt aaatctcaag ttggaaggtt
      421 tagtatatct acatacagaa actgataatt aaaaaagtag aaattaaaaa acttaatact
      481 aaagatatag ataaatatat gaggtaacag ctagagagag ttgttactct ttttaaatac
      541 taagtatata ttattcttat taattttttg taaattcact aaaagacctt tttatagaat
      601 attattttct ttttttatta aataggtaaa tcagtaaaaa tgcaattcca g
//
LOCUS       DAEPXF010000400          620 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain HB27
            SAMN13376389-rid12479423.denovo.409, whole genome shotgun sequence.
ACCESSION   DAEPXF010000400 DAEPXF010000000
VERSION     DAEPXF010000400.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13376389
            Sequence Read Archive: SRR10518432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 620)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 620)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001069624.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001069624.1
            Genome Coverage       :: 315x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/15/2021 01:54:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,165
            CDSs (total)                      :: 4,076
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 89
            rRNAs                             :: 10, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 10 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 19 of 69
            Pseudo Genes (incomplete)         :: 44 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 10 of 69
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..620
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13376389-rid12479423.denovo.409"
                     /strain="HB27"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="China: Hebei"
                     /collection_date="2017"
     gene            complement(80..559)
                     /locus_tag="KRL67_003984"
     CDS             complement(80..559)
                     /locus_tag="KRL67_003984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2975 domain-containing protein"
                     /protein_id="HBH1524760.1"
                     /translation="MSNKLIKNLLSGILQILFFLLGVVIVAGGFKSFMYLCFSGEATL
                     QGTIYGILMFILGVSYFIIIKSLIEVLGSSEHSLFVKENVKRFRLIGYLLLLNSIIEF
                     ISTFGTTGKGMRFLDLGFGFYFTVPVFVYFITSLMSFVIADGFVKAIKIKEDNDLTI"
BASE COUNT          248 a          105 c           45 g          222 t
ORIGIN      
        1 cttaatatat tttattttaa attactagtt aatcactctt ttcagattat ttatctccta
       61 ctatattaaa atgtcacgcc taaatagtta aatcattatc ctcttttatc tttattgcct
      121 ttacaaatcc atctgcaatt acaaaactca taagagaagt tataaaatat acaaatactg
      181 gaacagtaaa ataaaatcca aatcctaaat ctaggaatct cattcctttt ccagtagtac
      241 caaaagtgga aataaactca attatagagt ttaaaagcaa aagatatcca ataagtctaa
      301 atctttttac attctctttt acaaataagc tatgttcaga actacctaat acttctatca
      361 atgattttat aataataaaa tatgatacac ctaaaataaa cattaatatt ccataaatag
      421 taccttgtag agttgcttct ccactaaaac aaaggtacat aaaactttta aatcctcctg
      481 caacaattac tactcctaaa agaaaaaata aaatttgtaa tataccactt agtaaatttt
      541 ttataagttt attagacatc ataccaactc ctttaaaata atttatatat agtataatac
      601 atcatcttat atcgtttttc
//
LOCUS       DAEPXF010000401          610 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain HB27
            SAMN13376389-rid12479423.denovo.410, whole genome shotgun sequence.
ACCESSION   DAEPXF010000401 DAEPXF010000000
VERSION     DAEPXF010000401.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13376389
            Sequence Read Archive: SRR10518432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 610)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 610)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001069624.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001069624.1
            Genome Coverage       :: 315x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/15/2021 01:54:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,165
            CDSs (total)                      :: 4,076
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 89
            rRNAs                             :: 10, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 10 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 19 of 69
            Pseudo Genes (incomplete)         :: 44 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 10 of 69
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..610
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13376389-rid12479423.denovo.410"
                     /strain="HB27"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="China: Hebei"
                     /collection_date="2017"
     gene            123..485
                     /locus_tag="KRL67_003985"
     CDS             123..485
                     /locus_tag="KRL67_003985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HBH1524761.1"
                     /translation="MIKLYGYTKCSTVKKAKNWLKENNLEFEDIDMIQNPPSKEELKS
                     IYKTSGYDIKKFFNTSGMKYRELGLKDIVKTESDDKLLEILVSDGMLIKRPLLLDGKN
                     VLLGFKEDVWKSTLLKED"
BASE COUNT          265 a           42 c           92 g          211 t
ORIGIN      
        1 tttaataatt tgaaatatga taatttttta tgttgaaagc tcataatgat tatgatataa
       61 attggttata gtatacaaat tatgggtata acaataaaaa attagaaagg aatttttata
      121 aaatgattaa attatatgga tatacaaaat gctctactgt taaaaaagct aaaaattggc
      181 ttaaggaaaa taatttagaa tttgaagata tagatatgat tcaaaaccct cctagtaaag
      241 aggagttaaa atcaatttat aagactagtg gatatgatat aaagaaattt tttaacacaa
      301 gtggtatgaa atatagagaa ttgggtctta aagatattgt aaaaactgaa tctgatgata
      361 aacttttaga aatattggta agtgatggta tgcttattaa aagaccatta cttttggatg
      421 gtaaaaatgt attattaggc tttaaagaag atgtatggaa aagtactttg ctaaaagaag
      481 attagaaaga gataggtgtg ttgattaaaa ttattaatta atacacctat aaatttatgt
      541 attaaatcta atttttataa atattaatta gcaaattcaa atcaaaaaaa ttcatatcag
      601 caaacatatt
//
LOCUS       DAEPXF010000402          606 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain HB27
            SAMN13376389-rid12479423.denovo.411, whole genome shotgun sequence.
ACCESSION   DAEPXF010000402 DAEPXF010000000
VERSION     DAEPXF010000402.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13376389
            Sequence Read Archive: SRR10518432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 606)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 606)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001069624.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001069624.1
            Genome Coverage       :: 315x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/15/2021 01:54:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,165
            CDSs (total)                      :: 4,076
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 89
            rRNAs                             :: 10, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 10 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 19 of 69
            Pseudo Genes (incomplete)         :: 44 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 10 of 69
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..606
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13376389-rid12479423.denovo.411"
                     /strain="HB27"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="China: Hebei"
                     /collection_date="2017"
     gene            125..265
                     /locus_tag="KRL67_003986"
     CDS             125..265
                     /locus_tag="KRL67_003986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH1524762.1"
                     /translation="MENLMMSIIAGVIASYIYDKLKSHSERPTKSGWELNIKFQKSKS
                     SK"
BASE COUNT          254 a           59 c           85 g          208 t
ORIGIN      
        1 ttaaaatttt atgtttccaa tatttatgat ataatgaata tagcaaggaa taaatatttg
       61 aaaaagctat gagtggtgtt tccatagaag tttcttcact ttcttatgag aggaggtgaa
      121 aactatggaa aatctaatga tgagtattat agctggtgtt atagccagtt acatttacga
      181 taaattaaag agccactctg agcgaccaac taagagtggc tgggaactta atataaagtt
      241 ccagaaaagt aaatcttcaa aataattgtt gaaggaaaca tcactcaaag ttaagtaaaa
      301 tatgtttcct tgcttttatt atatcacaaa ttaaaaatat taacacaaaa ttttgtgaat
      361 atatacttat tattattcta aaaaaatatg gaaataattg atttgattgc atggaattat
      421 tgatgtaata tttagttaaa tatatacaat ctcaattaaa gaagataagt actataaatt
      481 catatcaaaa aaatacataa acttttatgt tataatttta taaaaagtct tttagtataa
      541 ttatttcaag aggaattaac atatgaaaaa ataacttcat tagaaaagtg gataaaaaac
      601 tggtaa
//
LOCUS       DAEPXF010000403          584 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain HB27
            SAMN13376389-rid12479423.denovo.412, whole genome shotgun sequence.
ACCESSION   DAEPXF010000403 DAEPXF010000000
VERSION     DAEPXF010000403.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13376389
            Sequence Read Archive: SRR10518432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 584)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 584)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001069624.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001069624.1
            Genome Coverage       :: 315x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/15/2021 01:54:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,165
            CDSs (total)                      :: 4,076
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 89
            rRNAs                             :: 10, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 10 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 19 of 69
            Pseudo Genes (incomplete)         :: 44 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 10 of 69
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..584
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13376389-rid12479423.denovo.412"
                     /strain="HB27"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="China: Hebei"
                     /collection_date="2017"
BASE COUNT          254 a           75 c           50 g          205 t
ORIGIN      
        1 tgttaataat agtaccataa ttaatatatc taatctggta gcggaagaaa tatctagaag
       61 ttttaagata tatttataaa atataaaaaa tgatatcata tttgctacta ttggtataca
      121 catataaatt tcttttttag tcacttgcaa atcattagat atgaatttat aaatgcataa
      181 caaaacaatt agaactacat ttcctaaaac tatagtttcg tatctataaa aattatcatt
      241 taataaaata ttcatagaac tcaaattatt tatcgaaact attactatca tacttaatgc
      301 atttatacac accagtattg cccagtaaga aaacaaaact attaaacatt tgtatatatt
      361 aacatcataa tttgatttat aaaaaacaaa cattacaaaa atgcatatta atattctatt
      421 atcagcacca acattagtat agaaagtaga aataataata aaagtggaaa ttaaaaaata
      481 taaatttatt aaactagttt ttactttact tgtgtaatca aacactttct tgcatgccca
      541 tatctctata atagaagaaa cgattgttat aaacatatct aaca
//
LOCUS       DAEPXF010000404          548 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain HB27
            SAMN13376389-rid12479423.denovo.413, whole genome shotgun sequence.
ACCESSION   DAEPXF010000404 DAEPXF010000000
VERSION     DAEPXF010000404.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13376389
            Sequence Read Archive: SRR10518432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 548)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 548)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001069624.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001069624.1
            Genome Coverage       :: 315x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/15/2021 01:54:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,165
            CDSs (total)                      :: 4,076
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 89
            rRNAs                             :: 10, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 10 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 19 of 69
            Pseudo Genes (incomplete)         :: 44 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 10 of 69
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..548
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13376389-rid12479423.denovo.413"
                     /strain="HB27"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="China: Hebei"
                     /collection_date="2017"
     gene            complement(<1..>548)
                     /locus_tag="KRL67_003987"
     CDS             complement(<1..>548)
                     /locus_tag="KRL67_003987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH1524763.1"
                     /translation="TILCVFICLSSVFIFTQLARVFINKKKINQKFKSRKGFRYDRDF
                     IEARREEINIKDNNNNKNKSNNKKLKEEKVFKYDNGDLYKGEFVDGKKNGFGIYIFSS
                     KEKYEGLWKDDKMHGIGKYTYRDGSIYTGEFKYGLKNGLGKLTYPNNDIYKGYFLDNK
                     KNGKGVLYKNDGNKQAGIWEDD"
BASE COUNT          165 a          105 c           28 g          250 t
ORIGIN      
        1 catcatcttc ccatattcct gcttgtttat ttccatcatt tttatataat acacccttac
       61 catttttctt attatctaaa aaatatccct tataaatatc attattggga tatgttaatt
      121 ttcccaaacc attttttaat ccgtatttaa attcgcctgt atatattgaa ccatctctat
      181 aagtatattt tcctattcca tgcatcttgt catctttcca taatccttca tacttttcct
      241 tacttgaaaa aatatatatt ccaaatccat ttttctttcc atcaacaaat tcacctttat
      301 ataaatcacc attatcgtat ttaaatactt tttcttcctt taatttctta ttgttactct
      361 tatttttatt attattgtta tcctttatat ttatttcttc tcttctagct tcaataaaat
      421 ctctatcata cctaaatcct tttcttgatt taaatttttg attaatcttc ttcttattaa
      481 taaatactct agctagttga gtaaatataa atacagatga taaacatata aatacacata
      541 gtatcgtt
//
LOCUS       DAEPXF010000405          501 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain HB27
            SAMN13376389-rid12479423.denovo.414, whole genome shotgun sequence.
ACCESSION   DAEPXF010000405 DAEPXF010000000
VERSION     DAEPXF010000405.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13376389
            Sequence Read Archive: SRR10518432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 501)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 501)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001069624.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001069624.1
            Genome Coverage       :: 315x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/15/2021 01:54:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,165
            CDSs (total)                      :: 4,076
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 89
            rRNAs                             :: 10, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 10 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 19 of 69
            Pseudo Genes (incomplete)         :: 44 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 10 of 69
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..501
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13376389-rid12479423.denovo.414"
                     /strain="HB27"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="China: Hebei"
                     /collection_date="2017"
     gene            <1..>501
                     /locus_tag="KRL67_003988"
     rRNA            <1..>501
                     /locus_tag="KRL67_003988"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          135 a          103 c          166 g           97 t
ORIGIN      
        1 agggtgatcg gccacattgg aactgagaca cggtccaaac tcctacggga ggcagcagtg
       61 gggaatattg cacaatgggc gaaagcctga tgcagcaacg ccgcgtgagt gatgaaggcc
      121 ttcgggtcgt aaaactctgt cctcaaggaa gataatgacg gtacttgagg aggaagcccc
      181 ggctaactac gtgccagcag ccgcggtaat acgtaggggg ctagcgttat ccggatttac
      241 tgggcgtaaa gggtgcgtag gcggtctttc aagtcaggag tgaaaggcta cggctcaacc
      301 gtagtaagct cttgaaactg ggagacttga gtgcaggaga ggagagtgga attcctagtg
      361 tagcggtgaa atgcgtagat attaggagga acaccagttg cgaaggcggc tctctggact
      421 gtaactgacg ctgaggcacg aaagcgtggg gagcaaacag gattagatac cctggtagtc
      481 cacgctgtaa acgatgagta c
//
LOCUS       DAEPXF010000406          492 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain HB27
            SAMN13376389-rid12479423.denovo.415, whole genome shotgun sequence.
ACCESSION   DAEPXF010000406 DAEPXF010000000
VERSION     DAEPXF010000406.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13376389
            Sequence Read Archive: SRR10518432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 492)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 492)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001069624.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001069624.1
            Genome Coverage       :: 315x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/15/2021 01:54:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,165
            CDSs (total)                      :: 4,076
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 89
            rRNAs                             :: 10, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 10 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 19 of 69
            Pseudo Genes (incomplete)         :: 44 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 10 of 69
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..492
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13376389-rid12479423.denovo.415"
                     /strain="HB27"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="China: Hebei"
                     /collection_date="2017"
     gene            complement(151..450)
                     /locus_tag="KRL67_003989"
     CDS             complement(151..450)
                     /locus_tag="KRL67_003989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH1524764.1"
                     /translation="MKWIKWYSITCICIFIVVAFYMFIFPNKIETIDTSSAYSFVEKK
                     VPNSAVYQGYKKNPVDGTTTIYYSYDNSTHIVRLSHPEDYSREINWDKVSNIRFD"
BASE COUNT          186 a           70 c           48 g          188 t
ORIGIN      
        1 atattttata aactaatata ttttacaaat tgttgagtaa tattttatgc tataaatcag
       61 aagaagcact tctataatag aaagtgctca actatgaata agattaataa tatatttgcc
      121 ttatatatag acttttataa atattaaaga ttaatcaaat ctgatgtttg aaactttatc
      181 ccaattaatc tcccttgaat agtcttctgg atgtgataat ctaactatat gagttgaatt
      241 atcataactg taataaattg tagtagtacc atcaactgga ttctttttgt atccttgata
      301 cacagcacta tttggtactt tcttttcaac aaaactatat gcagatgaag tatcaatagt
      361 ttctatttta ttaggaaata taaacatata aaaagctact actataaaaa tacatatgca
      421 tgtaatacta taccatttaa tccatttcat aaaattcctc ctactttacc aaaatatact
      481 attattattt cc
//
LOCUS       DAEPXF010000407          492 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain HB27
            SAMN13376389-rid12479423.denovo.416, whole genome shotgun sequence.
ACCESSION   DAEPXF010000407 DAEPXF010000000
VERSION     DAEPXF010000407.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13376389
            Sequence Read Archive: SRR10518432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 492)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 492)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001069624.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001069624.1
            Genome Coverage       :: 315x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/15/2021 01:54:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,165
            CDSs (total)                      :: 4,076
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 89
            rRNAs                             :: 10, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 10 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 19 of 69
            Pseudo Genes (incomplete)         :: 44 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 10 of 69
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..492
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13376389-rid12479423.denovo.416"
                     /strain="HB27"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="China: Hebei"
                     /collection_date="2017"
     gene            complement(<1..>492)
                     /locus_tag="KRL67_003990"
     CDS             complement(<1..>492)
                     /locus_tag="KRL67_003990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH1524765.1"
                     /translation="LLDYKFYKNNDRFRDCYLLARLLLLNLSMDNSQNNQEAFSILFE
                     INTLYEEYIGILIKSIWDNSFRETYIQDKSKFLLKNEQTGKKNFNLRPDIVLYDLKNE
                     YEIIIDTKWKAIEVDSNVFYRSSDIYQMYAYITAYENAKRCILLYPCIQKDKNYSSWK
                     LSES"
BASE COUNT          174 a           69 c           39 g          210 t
ORIGIN      
        1 gattctgata atttccaaga actataattc ttatcttttt gaatacaagg atataataat
       61 atacatcttt ttgcattttc atatgctgtt atatatgcat acatctggta tatatcactt
      121 gacctataaa aaacatttga atccacttca atagctttcc atttagtgtc aattataatt
      181 tcatattcat tttttaaatc atataaaact atatctggtc ttaaattaaa gtttttctta
      241 cctgtttgtt catttttcaa aagaaattta ctcttatctt gtatatatgt ttctctaaat
      301 gaattatccc atatactttt gattaaaatt cctatatatt cttcatacaa agtatttatt
      361 tcaaataata tagaaaatgc ctcttgatta ttttgactat tatccataga caaatttaaa
      421 agtagaagtc tagctagtaa ataacaatct ctaaatctat cattgttttt ataaaattta
      481 taatctaata at
//
LOCUS       DAEPXF010000408          476 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain HB27
            SAMN13376389-rid12479423.denovo.417, whole genome shotgun sequence.
ACCESSION   DAEPXF010000408 DAEPXF010000000
VERSION     DAEPXF010000408.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13376389
            Sequence Read Archive: SRR10518432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 476)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 476)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001069624.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001069624.1
            Genome Coverage       :: 315x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/15/2021 01:54:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,165
            CDSs (total)                      :: 4,076
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 89
            rRNAs                             :: 10, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 10 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 19 of 69
            Pseudo Genes (incomplete)         :: 44 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 10 of 69
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..476
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13376389-rid12479423.denovo.417"
                     /strain="HB27"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="China: Hebei"
                     /collection_date="2017"
     gene            <1..455
                     /locus_tag="KRL67_003991"
     CDS             <1..455
                     /locus_tag="KRL67_003991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBH1524766.1"
                     /translation="LKGVGDNSQMIKSLGINIGSIKILGLMISNGLIALSGSLMAQFL
                     GFSDVNMGIGTLVLGIASIIIGITLFKKFTFIKDTTAIIVGSFIYQFTIYFAMSLGML
                     STDLKLITAIVIIAFLATGNLNISLKKNKCKASTKNKSEKRGVVDVTN"
BASE COUNT          169 a           66 c           72 g          169 t
ORIGIN      
        1 ctttaaaagg tgttggtgat aactctcaaa tgattaaatc tctgggtata aatatcggtt
       61 ctataaaaat actaggactt atgatttcaa atggacttat agctttatct ggtagtctta
      121 tggctcagtt cttgggattc tcagatgtaa atatgggtat aggaacttta gttcttggaa
      181 ttgcctctat cataattggt attacactat ttaaaaaatt tacttttatc aaagatacta
      241 ctgcaataat tgttggctct tttatatacc aattcactat atactttgca atgagtttag
      301 ggatgttatc aactgactta aaattaataa ctgccatagt tataatagct ttcttagcta
      361 ctggtaactt aaatatttca ctaaaaaaaa acaaatgcaa agctagtacc aaaaataaat
      421 cagaaaaaag aggtgttgta gatgttacaa attaaaaatc tttcaaagag tttccc
//
LOCUS       DAEPXF010000409          473 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain HB27
            SAMN13376389-rid12479423.denovo.418, whole genome shotgun sequence.
ACCESSION   DAEPXF010000409 DAEPXF010000000
VERSION     DAEPXF010000409.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13376389
            Sequence Read Archive: SRR10518432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 473)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 473)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001069624.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001069624.1
            Genome Coverage       :: 315x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/15/2021 01:54:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,165
            CDSs (total)                      :: 4,076
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 89
            rRNAs                             :: 10, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 10 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 19 of 69
            Pseudo Genes (incomplete)         :: 44 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 10 of 69
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..473
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13376389-rid12479423.denovo.418"
                     /strain="HB27"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="China: Hebei"
                     /collection_date="2017"
     gene            168..284
                     /gene="rrf"
                     /locus_tag="KRL67_003992"
     rRNA            168..284
                     /gene="rrf"
                     /locus_tag="KRL67_003992"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            290..378
                     /locus_tag="KRL67_003993"
     tRNA            290..378
                     /locus_tag="KRL67_003993"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:324..326,aa:Leu,seq:taa)
     gene            390..466
                     /locus_tag="KRL67_003994"
     tRNA            390..466
                     /locus_tag="KRL67_003994"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:424..426,aa:Ile,seq:cat)
BASE COUNT          150 a           90 c          106 g          127 t
ORIGIN      
        1 agtttttaga gtgctaactc tatattttta aacttgtcaa aacttacaga tttttaatag
       61 ttttataagg caaaaaatcg aataaaaaaa gtattgacac aataagacaa agatgttatt
      121 ataatacttg tcttagaaaa aaagataaat gaaaatttag acaataatct ggttataata
      181 gcagagagga tacacctgtt cccattccga acacagaagt taagctctct agcgctgatg
      241 gtacttggtg ggaaactgcc tgggagagta ggacatagcc aggtgatgtg ccgaagtggc
      301 ggaactggca gacgcacagg acttaaaatc ctgcgggact tacctctcgt accggttcga
      361 ttccggtctt cggcaccaca aattattgtg ggccattagc tcagttggtt agagcgcccg
      421 gctcataacc ggtaggtctg gggttcgagt ccctgatggc ccaccacaat aat
//
LOCUS       DAEPXF010000410          471 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain HB27
            SAMN13376389-rid12479423.denovo.419, whole genome shotgun sequence.
ACCESSION   DAEPXF010000410 DAEPXF010000000
VERSION     DAEPXF010000410.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13376389
            Sequence Read Archive: SRR10518432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 471)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 471)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001069624.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001069624.1
            Genome Coverage       :: 315x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/15/2021 01:54:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,165
            CDSs (total)                      :: 4,076
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 89
            rRNAs                             :: 10, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 10 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 19 of 69
            Pseudo Genes (incomplete)         :: 44 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 10 of 69
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..471
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13376389-rid12479423.denovo.419"
                     /strain="HB27"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="China: Hebei"
                     /collection_date="2017"
     gene            105..326
                     /locus_tag="KRL67_003995"
     CDS             105..326
                     /locus_tag="KRL67_003995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH1524767.1"
                     /translation="MDRLKRDMKNMKEDLKEGVKKTAYVMKNTAEDLKDDAQGAMVNL
                     EMKKDEMMERYEQKKFSKELEKQMKNEMK"
BASE COUNT          218 a           27 c           81 g          145 t
ORIGIN      
        1 ctatatttaa gacttaaaaa ttataattga tattacaaat aaatatatgt actttgggaa
       61 aaataaatat aggaagtatt atatttacag aggaggtagt tttgatggat agattaaaaa
      121 gagatatgaa aaatatgaaa gaagatttaa aagaaggtgt taaaaagaca gcttatgtta
      181 tgaaaaatac agctgaagat ttgaaagatg atgcacaagg tgctatggta aacttagaaa
      241 tgaaaaaaga tgagatgatg gaaagatatg aacaaaagaa attttctaaa gaattagaga
      301 agcaaatgaa gaatgaaatg aaataagctt ttatttatga ataacaaatt attgtattca
      361 tataaaaagt agccagtata ttaaatagtt tatttaagga aatagacttt ctaaaaataa
      421 ctgtattaga tttagaaagt ctatttcaaa ttagttttat gaatagacat a
//
LOCUS       DAEPXF010000411          460 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain HB27
            SAMN13376389-rid12479423.denovo.420, whole genome shotgun sequence.
ACCESSION   DAEPXF010000411 DAEPXF010000000
VERSION     DAEPXF010000411.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13376389
            Sequence Read Archive: SRR10518432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 460)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 460)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001069624.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001069624.1
            Genome Coverage       :: 315x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/15/2021 01:54:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,165
            CDSs (total)                      :: 4,076
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 89
            rRNAs                             :: 10, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 10 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 19 of 69
            Pseudo Genes (incomplete)         :: 44 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 10 of 69
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..460
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13376389-rid12479423.denovo.420"
                     /strain="HB27"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="China: Hebei"
                     /collection_date="2017"
     gene            45..>460
                     /locus_tag="KRL67_003996"
     CDS             45..>460
                     /locus_tag="KRL67_003996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBH1524768.1"
                     /translation="MIAVKKAYKFRMYPNKKQQELINKTFGCCRFVYNKYLAKRIEVY
                     KNNKETFTYKQCSSDLTNLKKELNWLKEPDKFSLQNALKDLENAYEKFFKEKTGFPKF
                     KSKKTNRFSYKTNFTNGNIMYCGQHIKLPKLGMVKI"
BASE COUNT          213 a           48 c           62 g          137 t
ORIGIN      
        1 tgataaaatc attaaaaagt gtatcaaaaa acaattaaag tgaggtgata gcagtgaaaa
       61 aagcttataa gtttagaatg tatccaaata aaaaacagca agagttaatt aacaaaactt
      121 ttggatgttg tagatttgta tataataaat atcttgctaa aagaatagaa gtttataaaa
      181 acaataaaga aacttttact tataaacagt gtagttctga tttgactaat ttaaaaaaag
      241 aattaaattg gcttaaagaa cctgacaaat tttcactaca aaacgcctta aaagatttag
      301 aaaacgcata tgaaaaattc tttaaagaaa agacaggatt tcctaaattt aaatcaaaga
      361 aaactaatag attttcatat aaaactaact ttacaaatgg aaacattatg tattgtggtc
      421 aacatataaa actacctaaa cttggtatgg ttaagataag
//
LOCUS       DAEPXF010000412          452 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain HB27
            SAMN13376389-rid12479423.denovo.421, whole genome shotgun sequence.
ACCESSION   DAEPXF010000412 DAEPXF010000000
VERSION     DAEPXF010000412.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13376389
            Sequence Read Archive: SRR10518432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 452)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 452)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001069624.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001069624.1
            Genome Coverage       :: 315x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/15/2021 01:54:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,165
            CDSs (total)                      :: 4,076
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 89
            rRNAs                             :: 10, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 10 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 19 of 69
            Pseudo Genes (incomplete)         :: 44 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 10 of 69
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..452
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13376389-rid12479423.denovo.421"
                     /strain="HB27"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="China: Hebei"
                     /collection_date="2017"
     gene            complement(<1..424)
                     /locus_tag="KRL67_003997"
     CDS             complement(<1..424)
                     /locus_tag="KRL67_003997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SufBD protein"
                     /protein_id="HBH1524769.1"
                     /translation="MKNLNLLINKLYSKNHNEAYKTFLFLENESLKSNITYCFFDSFL
                     EMIDNKNSYIRTRGLLLISANAQWDIDNKIEINIDSILSHIVDKKPSVSRMFIKSIPN
                     ITKYKENLINRIKMELSNADISIYNNNMKPLVEKDINDT"
BASE COUNT          150 a           58 c           44 g          200 t
ORIGIN      
        1 atgtatcgtt tatatccttt tctactaatg gtttcatatt attattataa atacttatat
       61 ctgcattact taattccatt tttatgcgat taataagatt ttctttatac tttgtaatat
      121 taggtatgga tttaataaac attcttgata cagaaggttt tttatctacg atatgactta
      181 gtatactatc tatatttatt tcaattttat tatcaatatc ccactgagca tttgcagaga
      241 ttaataacaa tccccttgtt cgaatatatg agtttttgtt atcaatcatt tctaggaatg
      301 agtcaaaaaa acaatatgta atatttgatt ttaaactttc attttctaag aataaaaaag
      361 ttttatatgc ttcattatga ttttttgaat ataatttatt aataagtaaa ttgagattct
      421 tcaatcttct catctccctc attacaaata tt
//
LOCUS       DAEPXF010000413          437 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain HB27
            SAMN13376389-rid12479423.denovo.422, whole genome shotgun sequence.
ACCESSION   DAEPXF010000413 DAEPXF010000000
VERSION     DAEPXF010000413.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13376389
            Sequence Read Archive: SRR10518432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 437)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 437)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001069624.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001069624.1
            Genome Coverage       :: 315x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/15/2021 01:54:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,165
            CDSs (total)                      :: 4,076
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 89
            rRNAs                             :: 10, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 10 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 19 of 69
            Pseudo Genes (incomplete)         :: 44 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 10 of 69
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..437
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13376389-rid12479423.denovo.422"
                     /strain="HB27"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="China: Hebei"
                     /collection_date="2017"
     gene            1..>437
                     /locus_tag="KRL67_003998"
     CDS             1..>437
                     /locus_tag="KRL67_003998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HBH1524770.1"
                     /translation="MKNILKENHITFEEYDHEPILSFEKAKEIREKLQYTGVESKSLF
                     IKDRSGNYYIFVTTEGKKLDPKTIKNVLGGKVSICSKEELTQKTQCLPGCVSPFGYEK
                     NISLVIDQDIFNHDKIIFSPGIPEKTFIITASGFKKVINNINNT"
BASE COUNT          201 a           49 c           60 g          127 t
ORIGIN      
        1 atcaaaaata ttttaaaaga aaatcatata acgtttgaag aatatgacca tgaacccatt
       61 ttatcatttg aaaaagctaa agaaataaga gaaaaattac aatatactgg agtagaaagt
      121 aaaagtttat ttataaaaga taggtctgga aattactata tatttgtaac cacagaagga
      181 aaaaaattag atccaaaaac tataaaaaat gttttaggtg gaaaagtatc tatatgttca
      241 aaagaagaat taacacaaaa aacacaatgt ttgcctggat gtgtatctcc ttttggatat
      301 gagaaaaata tctctcttgt aatagatcaa gatatattta accatgataa aataatcttt
      361 tcaccaggga taccagaaaa gacatttatt attacagcta gtggatttaa aaaagtaata
      421 aacaatataa acaatac
//
LOCUS       DAEPXF010000414          430 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain HB27
            SAMN13376389-rid12479423.denovo.423, whole genome shotgun sequence.
ACCESSION   DAEPXF010000414 DAEPXF010000000
VERSION     DAEPXF010000414.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13376389
            Sequence Read Archive: SRR10518432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 430)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 430)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001069624.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001069624.1
            Genome Coverage       :: 315x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/15/2021 01:54:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,165
            CDSs (total)                      :: 4,076
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 89
            rRNAs                             :: 10, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 10 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 19 of 69
            Pseudo Genes (incomplete)         :: 44 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 10 of 69
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..430
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13376389-rid12479423.denovo.423"
                     /strain="HB27"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="China: Hebei"
                     /collection_date="2017"
     gene            35..373
                     /locus_tag="KRL67_003999"
     CDS             35..373
                     /locus_tag="KRL67_003999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2325 domain-containing protein"
                     /protein_id="HBH1524771.1"
                     /translation="MNLLVIGGHERMEKDYMVMAKKKGYKTKVYTTMSSKLNNSIGRP
                     DAVVILTSTVSHKMSRTVESQAKKQDILIVRHKNSSKVAFNECLDMVDECLGNCSKCK
                     LKDKQKQKNI"
BASE COUNT          193 a           44 c           74 g          119 t
ORIGIN      
        1 gaaaatcaat atcaaataaa tgggaggaaa tctaatgaac ttattagtga ttggtggtca
       61 tgaaagaatg gaaaaagatt atatggttat ggccaagaaa aaaggttata agacaaaggt
      121 atatactacc atgtcatcta aattaaataa ttctatagga agaccagatg cagttgtcat
      181 acttacatca actgtttcac ataagatgtc tagaacggtt gagtctcaag ctaaaaaaca
      241 agatatatta atcgtaaggc ataaaaatag tagtaaagta gcatttaatg aatgcctaga
      301 tatggttgat gaatgtctgg gaaattgttc aaaatgtaag cttaaagaca aacaaaaaca
      361 aaaaaatata taggtataaa aaatacataa acataaaatt aaaagtactg agataatagt
      421 tataattatt
//
LOCUS       DAEPXF010000415          414 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain HB27
            SAMN13376389-rid12479423.denovo.424, whole genome shotgun sequence.
ACCESSION   DAEPXF010000415 DAEPXF010000000
VERSION     DAEPXF010000415.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13376389
            Sequence Read Archive: SRR10518432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 414)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 414)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001069624.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001069624.1
            Genome Coverage       :: 315x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/15/2021 01:54:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,165
            CDSs (total)                      :: 4,076
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 89
            rRNAs                             :: 10, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 10 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 19 of 69
            Pseudo Genes (incomplete)         :: 44 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 10 of 69
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..414
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13376389-rid12479423.denovo.424"
                     /strain="HB27"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="China: Hebei"
                     /collection_date="2017"
     gene            20..325
                     /locus_tag="KRL67_004000"
     CDS             20..325
                     /locus_tag="KRL67_004000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH1524772.1"
                     /translation="MPSIIMEVVGVLFIAIGLILTFIEIKGNFPSFYGFGQNSIGKNA
                     IGIIAAGIALIFFRNGIDSIDSIATYGVFLAIELVVIVAYFLIRKKFTKEDKHLRTN"
BASE COUNT          159 a           37 c           63 g          155 t
ORIGIN      
        1 ttaataagga ggggtcaaaa tgcctagtat aattatggaa gttgtaggag tactatttat
       61 agctattgga ttaatcttga cattcattga aataaaagga aatttccctt ctttttatgg
      121 ttttggtcaa aattctatag ggaaaaatgc tataggtatt atagcagcag gaattgcttt
      181 aattttcttt agaaatggca tagatagcat agattctata gctacttatg gtgtattttt
      241 agctatagaa cttgttgtaa tagttgcata tttcttaata agaaaaaaat ttactaaaga
      301 agataaacat ttaagaacta attaactaaa ttatctacta taaaattgga aaattaaaaa
      361 caaataaaag tttgttttta attatttaaa atccaaattt gattattttt aaag
//
LOCUS       DAEPXF010000416          378 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain HB27
            SAMN13376389-rid12479423.denovo.426, whole genome shotgun sequence.
ACCESSION   DAEPXF010000416 DAEPXF010000000
VERSION     DAEPXF010000416.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13376389
            Sequence Read Archive: SRR10518432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 378)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 378)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001069624.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001069624.1
            Genome Coverage       :: 315x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/15/2021 01:54:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,165
            CDSs (total)                      :: 4,076
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 89
            rRNAs                             :: 10, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 10 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 19 of 69
            Pseudo Genes (incomplete)         :: 44 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 10 of 69
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..378
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13376389-rid12479423.denovo.426"
                     /strain="HB27"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="China: Hebei"
                     /collection_date="2017"
     gene            <1..230
                     /locus_tag="KRL67_004001"
     CDS             <1..230
                     /locus_tag="KRL67_004001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HBH1524773.1"
                     /translation="LTPTENRLLNYFIENAEKTLTIKEIYEHVWMNEYLDDNYSVVVA
                     VSGLRKKIEKDYKNPQKIITIREVGYYFNKI"
BASE COUNT          168 a           26 c           62 g          122 t
ORIGIN      
        1 agttaactcc aacagaaaat agattattaa attactttat agagaatgct gaaaagactc
       61 taactattaa agaaatatat gaacatgtat ggatgaatga gtatttagat gataattata
      121 gtgtagtggt tgctgtgagt ggattaagaa agaaaataga gaaagattat aaaaatcctc
      181 aaaaaattat aacaataaga gaagtagggt attactttaa caagatataa agctttataa
      241 atagttttat aaatagaatg aatgataagg catagataat atgttagtat attacaagag
      301 tttctgtcct aacgatatat ttgtaaaatt agatttagtt tacttaatta aattatagca
      361 gtagaataag tataaaaa
//
LOCUS       DAEPXF010000417          326 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain HB27
            SAMN13376389-rid12479423.denovo.427, whole genome shotgun sequence.
ACCESSION   DAEPXF010000417 DAEPXF010000000
VERSION     DAEPXF010000417.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13376389
            Sequence Read Archive: SRR10518432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 326)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 326)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001069624.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001069624.1
            Genome Coverage       :: 315x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/15/2021 01:54:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,165
            CDSs (total)                      :: 4,076
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 89
            rRNAs                             :: 10, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 10 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 19 of 69
            Pseudo Genes (incomplete)         :: 44 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 10 of 69
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..326
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13376389-rid12479423.denovo.427"
                     /strain="HB27"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="China: Hebei"
                     /collection_date="2017"
BASE COUNT          131 a           26 c           46 g          123 t
ORIGIN      
        1 gttaaagata agaatgtata aggtggtaaa tttgtataaa aaagaatatg agttgtaaaa
       61 tattaaataa tattgtacta attggaataa ttttaacttt attatagatt attttaatac
      121 cattagtact gatgatgtat tttaaaagtg gtataagcat tacaaatacg aatatgccaa
      181 ctgtaatatg taatataagt agctatatac atatgtgttg tcccatatat tttatcattg
      241 tttatattaa aaagcttatg taaaacagaa gaaagagagg gagacactat tatatccctc
      301 ttttttctat agtaattaat tattaa
//
LOCUS       DAEPXF010000418          295 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain HB27
            SAMN13376389-rid12479423.denovo.428, whole genome shotgun sequence.
ACCESSION   DAEPXF010000418 DAEPXF010000000
VERSION     DAEPXF010000418.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13376389
            Sequence Read Archive: SRR10518432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 295)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 295)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001069624.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001069624.1
            Genome Coverage       :: 315x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/15/2021 01:54:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,165
            CDSs (total)                      :: 4,076
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 89
            rRNAs                             :: 10, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 10 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 19 of 69
            Pseudo Genes (incomplete)         :: 44 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 10 of 69
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..295
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13376389-rid12479423.denovo.428"
                     /strain="HB27"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="China: Hebei"
                     /collection_date="2017"
     gene            complement(<1..268)
                     /locus_tag="KRL67_004002"
     rRNA            complement(<1..268)
                     /locus_tag="KRL67_004002"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT           71 a           78 c           52 g           94 t
ORIGIN      
        1 ggctaatccg cgttcgctcg ccgctactta cggaatcgaa tttctttctc ttccttcagg
       61 tacttagatg tttcagttcc ctgagttccc ctcattaagc tatgtattca cttaatgata
      121 cttagacatt actctaagtg agtttcctca ttcggaaatc ttcggatcaa agtttacgtg
      181 caactccccg aagcttatcg cagcttatca cgtccttcat cggctcctag tgccaaggca
      241 tccgccctgc acccttaata acttgaccag ttaaaaaggt ttgatagatt agaag
//
LOCUS       DAEPXF010000419          285 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain HB27
            SAMN13376389-rid12479423.denovo.429, whole genome shotgun sequence.
ACCESSION   DAEPXF010000419 DAEPXF010000000
VERSION     DAEPXF010000419.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13376389
            Sequence Read Archive: SRR10518432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 285)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 285)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001069624.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001069624.1
            Genome Coverage       :: 315x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/15/2021 01:54:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,165
            CDSs (total)                      :: 4,076
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 89
            rRNAs                             :: 10, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 10 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 19 of 69
            Pseudo Genes (incomplete)         :: 44 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 10 of 69
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..285
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13376389-rid12479423.denovo.429"
                     /strain="HB27"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="China: Hebei"
                     /collection_date="2017"
     gene            148..264
                     /gene="rrf"
                     /locus_tag="KRL67_004003"
     rRNA            148..264
                     /gene="rrf"
                     /locus_tag="KRL67_004003"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
BASE COUNT          101 a           42 c           54 g           88 t
ORIGIN      
        1 agtttttaga gtgttaactc tatgtaaagt actaacttca attgatgaag tatatatcgt
       61 aaaaacaatt actatttagt acttcaaaac tattgacatt taaaaataaa gatgttaata
      121 taatacttgt cttataaata ggcaatatgt ggttataata gcaaagagga tacacctgtt
      181 cccattccga acacagaagt taagctcttt agcgctgatg gtacttggtg ggaaactgcc
      241 tgggagagta ggacgtagcc acgtgaatat tccaaggtag ctcaa
//
LOCUS       DAEPXF010000420          272 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain HB27
            SAMN13376389-rid12479423.denovo.430, whole genome shotgun sequence.
ACCESSION   DAEPXF010000420 DAEPXF010000000
VERSION     DAEPXF010000420.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13376389
            Sequence Read Archive: SRR10518432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 272)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 272)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001069624.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001069624.1
            Genome Coverage       :: 315x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/15/2021 01:54:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,165
            CDSs (total)                      :: 4,076
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 89
            rRNAs                             :: 10, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 10 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 19 of 69
            Pseudo Genes (incomplete)         :: 44 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 10 of 69
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..272
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13376389-rid12479423.denovo.430"
                     /strain="HB27"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="China: Hebei"
                     /collection_date="2017"
     gene            <1..>272
                     /locus_tag="KRL67_004004"
     rRNA            <1..>272
                     /locus_tag="KRL67_004004"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT           73 a           50 c           90 g           59 t
ORIGIN      
        1 catacgggtt gaagcatgac cgtaaggaca tgtggacagt atgggagtga gaatgttggc
       61 atgagtagcg agatgtgggt gagaatccca caggccgtaa acccaaggtt tccaggggaa
      121 ggttcgtccg ccctgggtta gtcgggacct aagctgaggc cgaaaggcgt aggtgatgga
      181 caacaggttg atattcctgt actaccgata accgtttgag agaagggatg acacagtagg
      241 ataagctaag cacactgttg gttatgtgtg cc
//
LOCUS       DAEPXF010000421        48406 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain HB27
            SAMN13376389-rid12479423.guided.007, whole genome shotgun sequence.
ACCESSION   DAEPXF010000421 DAEPXF010000000
VERSION     DAEPXF010000421.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13376389
            Sequence Read Archive: SRR10518432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 48406)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 48406)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001069624.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001069624.1
            Genome Coverage       :: 315x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/15/2021 01:54:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,165
            CDSs (total)                      :: 4,076
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 89
            rRNAs                             :: 10, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 10 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 19 of 69
            Pseudo Genes (incomplete)         :: 44 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 10 of 69
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..48406
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13376389-rid12479423.guided.007"
                     /strain="HB27"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="China: Hebei"
                     /collection_date="2017"
     gene            complement(<1..749)
                     /locus_tag="KRL67_004005"
     CDS             complement(<1..749)
                     /locus_tag="KRL67_004005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBH1524774.1"
                     /translation="MNKLLYLINVDLRRSNKFYIAYISFFSLITLSLNLFEVNKFKNS
                     KFIMNLAIGDFGGIFYGIGILSNLGFIQSILFFGMLGLFIYYIFMWKREFFSNNQSIY
                     TLMMLPQNKFKICISKSIALITMIYGFLISQIATLFICKYIFNFIFRNMPIINMNFAK
                     DLYYLNLKSIPIDFISFMAIYVFLLLIAISIVNCCILFIFSYINKIYVILITLLIIAG
                     LYFSTIYINIITLFRKYYKVIGFQFNNLVAG"
     gene            complement(742..1458)
                     /locus_tag="KRL67_004006"
     CDS             complement(742..1458)
                     /locus_tag="KRL67_004006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBH1524775.1"
                     /translation="MIEIKNVSKTYKRMQGLKIRKIKALKNVSFNIEKGKITALLGIN
                     GVGKSTMLKAIAGLINIDSGEIRIDGEKINEKVYNKLAFVPDVQSHFSNTTIKETFEF
                     MEIFYSKWNKEKSKEMMDIFKLDEDEIIDNLSKGNIARVKLILGFCQDPEYILLDEPF
                     TGIDLFKREEFIGVIAQYMEENQAIIITTHEIVEIESLVDEVVILDEGQIITSFNAEE
                     LREREGKSILDKMREVYKNE"
     gene            complement(1529..1885)
                     /locus_tag="KRL67_004007"
     CDS             complement(1529..1885)
                     /locus_tag="KRL67_004007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBH1524776.1"
                     /translation="MNFELDNTTPIYLQIVKYIKRQIVIGELKPGETIPSRREMALNL
                     KVNLNTVQRAYKEMGDMNIINTFKNYQSSVTVDENILKNLKLELINESLSVFIEDMKA
                     INVSKEEVLKIIEDKY"
     gene            2241..2699
                     /locus_tag="KRL67_004008"
     CDS             2241..2699
                     /locus_tag="KRL67_004008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HBH1524777.1"
                     /translation="MSYKIEIKNIESVTVATMRYNGPMTEATKYFPNVFKAIKGKSNG
                     APFFCYYDVDQKTGVAEMELCVPTAETPNRMGITTKEFPKTKALFFTHIGSYSNLPKT
                     YEMIFKYIHENNIKIQTPWREVFIKGPGMLIKGNPDNYITEIIFPLKEEE"
     gene            2703..3665
                     /locus_tag="KRL67_004009"
     CDS             2703..3665
                     /locus_tag="KRL67_004009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBH1524778.1"
                     /translation="MLAIEIDNLVKEYKNGVKALNGLSFNVNSGEIFSLLGPNGAGKS
                     SLINILTTFYKPTSGNVTMFGKDLVDNPSWIRTQIACVAQQISIDEHLSLMENMVFQS
                     KMYKVEPQIAKQRIDSLIDKFDLSSYLKYPTSSYSGGVKRRLDIAMNMVSSPKILFLD
                     EPTVGMDVDSRKSMWDMLLKIRDEYGTTIFLTTHYLEEAEQLSDNICIMKNGKDLAQG
                     TPSSLRSYIRQNILRITFHNTEDIKKYKDAIKSTGLVKFMNVRENSIFISVNDSRTAF
                     TLINKWLLEHDIKFDAIEIVEPSLEDVFLALTSSKKDLKEGWEC"
     gene            3659..4408
                     /locus_tag="KRL67_004010"
     CDS             3659..4408
                     /locus_tag="KRL67_004010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBH1524779.1"
                     /translation="MLNILWRSMKWRFKNPISFVVTILQPFLWLVLYSSIANQTMNNI
                     NINNYTAFILPGIIVLVVFSSCSSGGIINFIMKNSGSFYRVLIAPISRYSIVLGQLLE
                     AILVSFIEVAILCIVSIFFSVRIESGIGGILLMIVLIFMTAFFLSSLAYSISLLLPNE
                     IVYETIMTAIVLPIFFLSSALFPIENLSGGLKVAVMLNPFTHVINALRSLIFGETILI
                     GDILPVILLFLILCCASFSLAMWRLKKEMVS"
     gene            complement(4474..4776)
                     /locus_tag="KRL67_004011"
     CDS             complement(4474..4776)
                     /locus_tag="KRL67_004011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBH1524780.1"
                     /translation="MIRILLVCVGGMSSTLLVNKMEKDAKKRNIDCKIWAVGEGNIKS
                     ELDNFDILLLGPQLRFMLDDVKSIVGDRAPVAIIDMVNYGTCNGHAVLNSVLEILK"
     gene            complement(4915..5547)
                     /gene="thiE"
                     /locus_tag="KRL67_004012"
     CDS             complement(4915..5547)
                     /gene="thiE"
                     /locus_tag="KRL67_004012"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HBH1524781.1"
                     /translation="MIDKEFLKKCLKLYLVTDSEMLKGRDFYKCLEDAISSGITTVQL
                     REKNASGREFLRKAMKLREITKRYGVKFIINDRVDIALICDADGVHVGQSDIEVREVR
                     KLIGNNKILGVSARTLEEAICAKNDGADYLGIGSIFTTSTKLDAKSASFETVKEIKEK
                     VDIPFVLIGGINLDNIDKLKCLESDGYAIISAILKAEDISKEVEKWTLKI"
     gene            complement(5570..6358)
                     /gene="thiM"
                     /locus_tag="KRL67_004013"
     CDS             complement(5570..6358)
                     /gene="thiM"
                     /locus_tag="KRL67_004013"
                     /EC_number="2.7.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyethylthiazole kinase"
                     /protein_id="HBH1524782.1"
                     /translation="MYNLIKDVKKLNPLVIHYTNNVTINDCANVTLAVGASPLMSFSY
                     EEVEEMVSVANSVVINIGTMNSNMLDLFLLAGKAANKYNKPVVLDPVGVFASKARAEL
                     TSRLLNEVKFSVVKGNVSEIKFIGGFNVRGKGVDSFDEEEDSTEIIRKIAEKLECVVV
                     ATGKIDIITNGKSTYKINNGTDKLKGITGTGCMTASLIASFMAVTENILEAATMGVLT
                     MSLSGELANLNNPPIGTFKENLMNAIYQMDIDTLSKNSNIEFLN"
     gene            complement(6382..7176)
                     /gene="thiD"
                     /locus_tag="KRL67_004014"
     CDS             complement(6382..7176)
                     /gene="thiD"
                     /locus_tag="KRL67_004014"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HBH1524783.1"
                     /translation="MSNYKIPTLTIAGSDSSGGAGIQADLKTFSAIGTYGMSVITAVT
                     AQNTKGVFDVEDLNKKIIKKQIEAVFEDIPPRAVKIGMVSSPEIILEIVENLKKYNPK
                     YLVVDPVMISKSGYYLLKPEAKENLIKHLIPLAYIITPNIPEAEEITGIKIHNVDDMK
                     RVGKEILQLGPKFVLMKGGHLDGEAVDILVGKNIFKVYKSERIDKKNTHGTGCTLSSA
                     ITSYLALGYEITEAVNLSKIYITEAIKRSFDIGHGVGPVHHFYKFE"
     regulatory      complement(7276..7368)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(7461..7772)
                     /locus_tag="KRL67_004015"
     CDS             complement(7461..7772)
                     /locus_tag="KRL67_004015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HBH1524784.1"
                     /translation="MYFTYIIRCKDDSLYTGYTSNIVRRMNEHKLGINSKYTRAKGFK
                     KLEVYFVTNTKSNAMKLEYYIKKLTRNKKLSIIKNPSILINLIDNKEDYIIGNEIEQL
                     T"
     gene            8062..9306
                     /locus_tag="KRL67_004016"
     CDS             8062..9306
                     /locus_tag="KRL67_004016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH1524785.1"
                     /translation="MENNLNTAKRNNTELSQNDSLKVNLQIGVDFTLLSKSLKAFYKK
                     EKDFYCIALAPCNVSSEKANRKISIYDMIKEINILIESITDKNELLSEELFTNNLSEF
                     CDKSLDEIYIDLKQAYLYIKKNTDSLNIEDSIEYVFDVDISNDIKAKENPLINFNSLS
                     LVVWSGETKHILQQMNILDLEDIHSYSMENNTNITIYENDTVQYKFDEESEDIKDSSI
                     DIIEQMLILPDDLNYNKDEVENMKSRLAKINIKYLQTLKEKDIKIKLINSNLTDEPEF
                     SDLKYQLPPCWVRSGKTWKDVPGIYRNNSIVAKIGYSNPSYANVHSSKNLELHETAHA
                     IDKNVLNKKSNSEEFIEVFAQERYKLYDPKQVAHAYISKFIEEFFAESFVHYYLDEDS
                     KNTLKENCPLTYDFLEKLELNY"
     gene            9503..10372
                     /locus_tag="KRL67_004017"
     CDS             9503..10372
                     /locus_tag="KRL67_004017"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl aminopeptidase"
                     /protein_id="HBH1524786.1"
                     /translation="MKLNRNDSCWCGSTKKYKKCHMDMDNKIDSFAKLGHIVPTHDII
                     KNESQIEEIKKSAVINTKILDYISDKIEIGMSTEDINKLVHDFTLQNGAIPAPLDYNG
                     FPKSVCTSVNNEVCHGIPSKDIILKDGDIINVDVSTIFNGYYSDASRMFMIGNVNENA
                     KKLVQVTKECVQKGLEVAKPWGFLGDIGDAINSYAKKNGYSVVREIGGHGVGLSFHED
                     PFVSYVSKKGTEMLLVPGMIFTIEPMVNEGTHEIFVDEENNWTIYTKDGKLSAQWECM
                     VLVTETGVEILTE"
     gene            complement(10426..10614)
                     /locus_tag="KRL67_004018"
     CDS             complement(10426..10614)
                     /locus_tag="KRL67_004018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HBH1524787.1"
                     /translation="MKANVNQDTCIGCGLCPSICPEVFDMKDDGKSHVIVDEVPSDAE
                     ESAAEARESCPVDAIDVH"
     gene            complement(10775..11365)
                     /gene="cysE"
                     /locus_tag="KRL67_004019"
     CDS             complement(10775..11365)
                     /gene="cysE"
                     /locus_tag="KRL67_004019"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HBH1524788.1"
                     /translation="MFKKINKDIEYIMKNDPAARSKIEVFLLYPSVHAMIMHRMAHAL
                     YKKKKLFTARLISQISRFMTGIEIHPGAKMGEGILIDHGMGVVIGETAEVGNRVTIYQ
                     GATLGATGKDTGKRHPTVGDDVLIGAGTKILGPLNIGSNSKIGANSVVVKDVPNGATV
                     VGIPAKIVKIRNLEPVKKNKKEVGYEYDELDNVYYI"
     gene            complement(11392..12300)
                     /gene="cysK"
                     /locus_tag="KRL67_004020"
     CDS             complement(11392..12300)
                     /gene="cysK"
                     /locus_tag="KRL67_004020"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HBH1524789.1"
                     /translation="MLYNNALELIGNTPVVRLNNLGCTNGCSNIYVKLEKVNPAGSIK
                     DRAVYEMLEGLEKRGELKKGDVLVEATSGNTGIALSMIGKLKGYEVVIVMPETMSVER
                     RDLMKAYGANLVLTDGKAGMAGSIEKANELLKENPNYKSLKQFENKDNPNAHYKTTAV
                     EIMNDVKDLDIFVCGVGTGGTLIGTARYLKEQNPNIRVIALEPKKSPAISENKTGPHK
                     IQGIGANFVPENYDSSVVDEVILVDDEDAFDTVKLLAAKEGILVGISSGANIFGAIEM
                     AKKYPDKKIVTVAPDGVDKYMSMGIF"
     gene            complement(12537..13163)
                     /locus_tag="KRL67_004021"
     CDS             complement(12537..13163)
                     /locus_tag="KRL67_004021"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit C"
                     /protein_id="HBH1524790.1"
                     /translation="MKTLKPALLVSIVLLVVCGLVYPLVLTSVSQVAFKDKANGSMIE
                     VNGVKVGSELIGQSFTDARFFKGRVSSVNYNTYTKEDLVPDKDGNTSYGGVSSGSFNY
                     GATNPELHDRVQKDIKQFLKDNPTVKKEDIPTDLLTASGSGLDPNISPASAKIQIPAV
                     SKASGISESKLQKIVDDNTSKKLFGVLGEDRVNVLKVNVEVAKILGLI"
     gene            complement(13182..15239)
                     /gene="kdpB"
                     /locus_tag="KRL67_004022"
     CDS             complement(13182..15239)
                     /gene="kdpB"
                     /locus_tag="KRL67_004022"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010966942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpB"
                     /protein_id="HBH1524791.1"
                     /translation="METKKTKFITKDIFKTSIIEAFKKLNPKYMMKNPVMFVVEIGFI
                     VTLILTIVPSLFGDEGNNLRAYNGIVTIILFITVLFANFAEAVAEGRGKAQADTLKKT
                     RKDTVARVIDTLGNEKMISASELKLGDIVLVNAGEVIPNDGEVIEGIASVDESAITGE
                     SAPVMRESGGDFASVTGGTTVVSDWLKIRITTKPGESFLDKMIALVEGASRQKTPNEI
                     ALNTLLVGLTLIFMVVLISLYPMANYAGVKIPISTMIALLVCLIPTTIGGLLSAIGIA
                     GMDRVTRFNVIAMSGKAVEACGDVDTMILDKTGTITYGNRLASEFIAVGNADSKDLIN
                     YSVMCSLKDDTPEGKSIVDLGMKLGSTGKTKEAEEAEFVEFSAQTRMSGINLADGRAI
                     RKGAYDSIIKRVKESGGTVPEDLEENVNRVAKLGGTPLVVCVDNEIYGIIYLKDTVKS
                     GLVERFARLREIGIKTIMCTGDNPLTAATIAREAGVDGFIAECKPEDKIDAIKKEQLE
                     GKIVAMTGDGTNDAPALAQADVGLAMNSGTTSAKEAANMVDLDSDPTKILEVVEIGKQ
                     LLITRGALTTFSIANDVAKYFAIIPAIFTLAIPQMEILNVMKLSTSFSAILSALIFNA
                     IIIPCLIPLAMKGVKYRPMKSQAMLMRNMLIFGLGGVIVPFLGIKLIDLLITPLVMVL
                     NLG"
     gene            complement(15264..16946)
                     /gene="kdpA"
                     /locus_tag="KRL67_004023"
     CDS             complement(15264..16946)
                     /gene="kdpA"
                     /locus_tag="KRL67_004023"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015391009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpA"
                     /protein_id="HBH1524792.1"
                     /translation="MFMVQIVIVLAIFMILVIPMGKYLYHIATNQKTFGDRLFDKVDN
                     FIYKVCSIDKKKEMNWKQYALALLFTNAVMVFIGYIILRTQSMHIFNPSGIKSMEQGL
                     SFNTIISFMTNTNLQHYSGESGLSYFSQMTVIIYMMFTSAATGYAAAMAFVRGLVGKK
                     KTLGNFYVDLIRITTRVLLPGALIIGLILVTQGVPQTFAGTETVTTIEGKLQDIARGP
                     VAALESIKHLGTNGGGFFGSNSSHPFENPTIISNIVEILSMMILPGACVVAFGHMIKN
                     KKQGWVVFGAMSIIFLIGLVVCFKAESAGNPILSQLGLNQSMGSMEGKEVRFGIAQSS
                     LFTTVTTSFTTGTVNNMHDTLTPLGGLVPLLNMMLNVVFGGKGVGLMNMLMYAIIAVF
                     LCGLMVGRTPEFLTKKIEGKEMKLIALLIILHPLLILMFSGLSVAIPAGLEGISNPGF
                     HGLSQVLYEFASSAANNGSGFEGLGDNTMFWNMTTGIVMFFGRYVSIIVLLAISSLLA
                     SKKAVNESIGTLRTDNFTFTIVLVLVVLIVGALTFFPALALGPISEHLVLWH"
     gene            complement(16987..17079)
                     /gene="kdpF"
                     /locus_tag="KRL67_004024"
     CDS             complement(16987..17079)
                     /gene="kdpF"
                     /locus_tag="KRL67_004024"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR02115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit F"
                     /protein_id="HBH1524793.1"
                     /translation="MEGLPMWFLAVVIIMLIIYLVYALINPEKF"
     gene            complement(17490..18122)
                     /locus_tag="KRL67_004025"
     CDS             complement(17490..18122)
                     /locus_tag="KRL67_004025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HBH1524794.1"
                     /translation="MARRLFLERIGLIFLGSAILAFGVYNFYYLNNITEGGVLGILLL
                     LKNLFNIQPAIANVVIDGLLLLVGYKFFGKKFLIYSIVASITFSVLYDLFEAIGPLVP
                     QSQNMLLSTILAGVTVGTGVGIVVKAGCASGGDDALALVISKTTSLNIGQVYLATDVI
                     VLLLSLFYLSAFDIFYSLIAVTISGKVIDFIYYHGKSLDMDISNDIVPEC"
     gene            complement(18488..19450)
                     /locus_tag="KRL67_004026"
     CDS             complement(18488..19450)
                     /locus_tag="KRL67_004026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HBH1524795.1"
                     /translation="MKKLMKHLALLISFIMIFSLVGCSNNGSKGKEGEKKIAVLLPGS
                     TGYFVATKQGIDAKSKELGVSVEYADAQWDASKQLSQAEDFMAKGVDMIALCGVDSAV
                     SERIVKAANDSDVPIIAFTNSIGSNPTGEFKGLVTYIGQNEEETGALTGKIAKNLLGE
                     AGGKAVLIEGVPGTTPQVLRKKGLEKELKDSNIEIVYNQTSNWEKEQALKVTEDLIQK
                     KTDFNVIICQDDNSATGAGQALKDAGIKDKVKVIGLGGSKDGLKAITDGLIDGTTYMS
                     AVKEGGLVIEKASKFLKGEKIEPVTQIKQVEVNKDNISEFKGEW"
     gene            complement(19474..20424)
                     /locus_tag="KRL67_004027"
     CDS             complement(19474..20424)
                     /locus_tag="KRL67_004027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBH1524796.1"
                     /translation="MKKAFEKLNLRDYGVLLGFLALCIAISIATPSFLGKQNILNLLR
                     QSSIIGIISAGMTFVIISGNFDISVGAVSALAGAITMKFATGGTNLFASMLLGIAICA
                     VIGLINGIVVAKINVPSLIATMAMVTIVRGLLLMLTGGYPITENIPILDYIGNGYLFN
                     IPIPVVIFFIVVLASFIVLNKTKFGRYVYSVGGNQEASKLNGINVDSHKVKVFIINAV
                     LAGIAGIVLTGRLGTATAIAGEGYDMDAIASVVIGGTSVAGGSGSVLKTVIGVLLMSV
                     INNSFNLLGIDVYFQYIFKGLIILAAVGFDSYSKKKLASR"
     gene            complement(20448..21944)
                     /locus_tag="KRL67_004028"
     CDS             complement(20448..21944)
                     /locus_tag="KRL67_004028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HBH1524797.1"
                     /translation="MSKILEMQQIYKSFGKVDVLKGINFELEKGEVRALLGANGAGKS
                     TLIKILGGVHSQTKGKVILAGNEIMFKNPHHSKENGISIIYQELSLIPTLTVLENVFL
                     GRELLKGSVFLNKKDMLEEYMKICEDFDFNIDPHIIVSNLSVANQQMVEIMKAVSCNS
                     EIIIMDEPTTSLTNNEKEGLFKIISRLKSKGKTIVYISHILEEVFKVCDSASIMKNGI
                     MIGTYDVEDLTKLKITQLMTGNDNHKLVEKKDYTFAKYEELPVLEVKNISWSNKIKNV
                     SFKVYRGEVVGLAGLVGSRRTEIINLIFGIDNKDSGDIFINGKKVDITSPKVAIKNKI
                     GLIPEDRKNLGLILEQAIYTNSSSVQIDKFKKFLFIDRNKEVEFSKKGVKELGIKVSD
                     VKQEVKELSGGNQQKVVVSKWLEKDLDLIIYDEPTKGIDIAAKEDIFNTIKEFSSKGI
                     GVIFISSDLEEVIRVSDRILVTREGSIVDDLKNENIQVQDIMNSIFNV"
     gene            complement(21957..23141)
                     /locus_tag="KRL67_004029"
     CDS             complement(21957..23141)
                     /locus_tag="KRL67_004029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH1524798.1"
                     /translation="MDRKKVIVQIFTGGFKTKEIKFNDIKCKLLELLDCIDIEKVIMG
                     WSVDEELYVETKKLLNKYNVELYLWLPCFSEIGLLEKSNLLIDYTGKEVKGYALQEDE
                     NFEFYCPNNKENIKNIKKIYSTYFSDIKFDGIFLDKIRYGSFSNKLNGVFNCFCKDCL
                     NIYEKNNIDKKSLMIEMNNMSKGVTDNEDTPFGITKYINGKYKFKNKLWEDFFETKNQ
                     NIYKALLEISSYFKSKGLKIGIDTFSPFISYFTGQDLEKLQEVADFIKPMMYRTTKAP
                     AGLPFEFERFIDESIYKNKEIAKRNFLEILGIKEYEGDVYPIEFVLEEIDFMTRSLNK
                     DIYVGVEINKKEIIAPVTPKYISENISNINKTTVKGYVLSWDLLSAPKENIDEIIKWF
                     RN"
     gene            complement(23134..24084)
                     /locus_tag="KRL67_004030"
     CDS             complement(23134..24084)
                     /locus_tag="KRL67_004030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine kinase"
                     /protein_id="HBH1524799.1"
                     /translation="MKYLVSIDGGGTKTKFCVSDLDGNILKEHTTGSTNYKSVGIKQT
                     YENINNGFKKILKDLYIDYDDIEYTVFGISGCDSPNDYKIIMDEILKIGINKEKIYLA
                     NDAVLAFYAQADSPGLVIVAGTGSIILGIKEDGEIYRVGGWGYNFSDLGSGYDIGRKL
                     LKKVLLYCDECHEYSDLFNCVLDFFGANSFEQLAYMITDINNNVEIANLASLVIDCAK
                     QGDKLAIEILRESSTELSKLAQVILSKISNPMKRDKSEINIVLSGGTLNKTVYAEMLM
                     NNLKIENKDKNLKFISQENQPVYGGIRLAMALANRRVKHG"
     gene            complement(24086..25180)
                     /locus_tag="KRL67_004031"
     CDS             complement(24086..25180)
                     /locus_tag="KRL67_004031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HBH1524800.1"
                     /translation="MKNIVGKPQTIKKVNEDLIKNIIKNEGPITKPEISSITELSLAT
                     VNKIVEQLALKNEVKVSGLSESTGGRRAQLFEINANLEHIIALYYYRNCCIGVVANLL
                     GEIIYQEEFNIRMDTYENVNQDTFFVIDTLIKKEKGNNIRAIGIGVPGVVKKGVVKNI
                     PTIKSWEGINLENLIKSRYNTKVFIENDTNITTIGIYQKQYKDKYKNMALMYLEDGIG
                     SGIVINGELYLGSTNFAGELSYLNIEFGKYNSNNLNLEDYIMSLRKDYLESKDNKFKK
                     EILSVISNFIRTLVCVLNPQAMIIQCSILTKSDMEFIEKDVSNSVGEENFPKLIKVDS
                     LREGSIHGVISMCLKETDYQYSLSNKKGGY"
     gene            25655..25924
                     /locus_tag="KRL67_004032"
     CDS             25655..25924
                     /locus_tag="KRL67_004032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH1524801.1"
                     /translation="MSYQNSIDLLPKELIEQVQEYIDGKVIYIPKKQEHKNHWGENTN
                     TKQVLASRNSQICINFQNGMSIKQLSEKYFLTEKSIQRILKQQNL"
     gene            26042..26794
                     /locus_tag="KRL67_004033"
     CDS             26042..26794
                     /locus_tag="KRL67_004033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBH1524802.1"
                     /translation="MSYFQYQGNSCFYKEYGQGKPIIFLHGNTGSSKMFKALMPLYVE
                     NFRCILIDFLGNGQSDRVSQFSPDMWHDEALQTIALIEHLNCGKVGLIGTSGGAWAAV
                     NTGLERPDLVEAIIADSFDGRTLNDNFIDNLITGREKSKQDIQARKFYEWCQGKDWEN
                     VVNLDTKALLQCANEKRPLFHKELCKLEMPILFTGSKEDEMCRHNLEEEYKQMATFIS
                     KASIHIFSQGGHPAILTNADEFAQLAKAFFKY"
     gene            complement(26999..28264)
                     /gene="hisD"
                     /locus_tag="KRL67_004034"
     CDS             complement(26999..28264)
                     /gene="hisD"
                     /locus_tag="KRL67_004034"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HBH1524803.1"
                     /translation="MKSIKTSSMKEKVDEKILREQVSSIIEDIRNNKDVALKKYNEKF
                     DRNTRDEFRITKEEIKEAYKHVDDEFINNLKIAAKNIREFAKAQKLSFENPFEKEIYP
                     GVILGQTNIPIESCLAYVPGGQYPLFSTALMLIIPAKVAGVKRIVACSPTMKNTEKIN
                     PKTLVAMDIAGADEIYATGGVQAIAAFTYGTEKINPVDIIVGPGNKFVTEAKRQCYGQ
                     VGIDFVAGPSEVLIIADETSNPVYIAADLLAQCEHDLNARGILLTNSLEIAQEVEKNI
                     ESMLKDLPTKDIAYSSWENNGEIILVDDMEEAIKISNFYAPEHLEVAVNEYNDICDRL
                     TNYGSLFIGNLSAEVFGDYVSGTNHTLPTLKASRYTGGVWVGTFIKTCTKQIFNEKAI
                     QSLAPVAEKLAKEEGLYAHAKAAEVRFKK"
     gene            complement(28529..29011)
                     /gene="cdeM"
                     /locus_tag="KRL67_004035"
     CDS             complement(28529..29011)
                     /gene="cdeM"
                     /locus_tag="KRL67_004035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exosporium morphogenetic protein CdeM"
                     /protein_id="HBH1524804.1"
                     /translation="MENKKCYSEDWYERGESTAKWFQNDREEYERAAYDEDRERRGSN
                     CGCSDSGENRPRNCERFRREAEIREREAREAFCESSEKKKEALAYECEARKLWEEAEK
                     YWDEYSKYNYQGIEYLAEAARLFDEGMECEARRNGNNGGNNNNCCHKCHKCNCNCCRK
                     "
     gene            complement(29128..30345)
                     /locus_tag="KRL67_004036"
     CDS             complement(29128..30345)
                     /locus_tag="KRL67_004036"
                     /EC_number="1.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HBH1524805.1"
                     /translation="MENKVKIGVLGYGVVGSGLIDIIDNNKEKRNIEIVGILVNNLEK
                     HKDKKYSNIITNNIDDIFNKDIDILVEVMGGLEPSLSYIKKALNNKIHVVTANKDLLA
                     ECGDELAKLASENKVSIKFEASVAGGIPVLKPIIESLEGNNIDSINAILNGTTNFILS
                     KMYDENLSYDMALRQAQELGFAEANPESDVLGYDAARKLSILSTLAYDNRVYWKDVYL
                     EGITDIDEKDIEYAKKLNCKIKLIGQSKYENDKVSAFVRPVLVEKDNILARIDNEFNA
                     VIVNGDSVGEVSFVGKGAGSLATGSAVYSDVIDIIDNRVSSIDSFTKDKIQVNKIVRE
                     KCGALLRFKKCNKDEILNIVENCLVKFDILNDDDELAIMVYADSEYEINNSLCLIKDK
                     GYCEKMNKMLKIS"
     misc_binding    complement(30430..30665)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     misc_binding    complement(30731..30971)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(31053..32909)
                     /locus_tag="KRL67_004037"
     CDS             complement(31053..32909)
                     /locus_tag="KRL67_004037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HBH1524806.1"
                     /translation="MDTHNKYVNFIKNIPVPFLYCRIVKRQEDIEYRVEYISKGMGKV
                     LQLEEGICDKNILDVLPVFKSKKYFKELFSNEVDCIKRYIPTLKNWINIKKQIIGDSY
                     IILYFGKIVFDYRQIIDSFDKKEKVAYIKDEEGIYIDCSENLIPILNNNIKTTKDIFG
                     KNDIEVWGENTGKLFRDDYREGVSSKKRFLQNLFEYEETFFMVEKYFLYDEDELLGTI
                     GIIDNIIYSDYSNRNYNSKDLMKMIEHSIPENMFYKDVYGNYIGFNSGFLNLACMNKE
                     ELLGKNSYKISEEEVLIDKIFESDKGVIENKKVVTFELNVSINDENKCIEITKRPFFD
                     SYGSVIGIIGTARDISRRKRLEEEMDKTRMEFFANLSHELRTPINLISSSLQVIEKKE
                     ADLIESNDTLKRNLGIIKQNGNRILRLVNNVIDFTKMQSGYLDFKPEESDIIAFIEEI
                     CMSVADFASQNNIQLTFDTEIEEFSMLFDSEKLERIILNLLSNGIKYNKEDGKINIFL
                     YVKDNIFNMKISDSGIGIPKEKIDKIFNRFEQIDNELSYRVKGSGIGLSLVKSLVELH
                     EGSISLKSQLGIGSEFIVSLPVRSKNNIEKYNHKREISNELSKKLEIEFSDL"
     gene            complement(33427..34266)
                     /locus_tag="KRL67_004038"
     CDS             complement(33427..34266)
                     /locus_tag="KRL67_004038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HBH1524807.1"
                     /translation="MFGIDKKLIEYIIAKSNYSELSSKSAEVSFFLMLSIFPFLIFTI
                     SSIAYIPILHLNKYIALFRNMMPEGAFAVLSSIIVSAIDNRNLKFLAVSFVLTMWTFS
                     RAVKALIKGMNRAYKVKETRSFLKILSISFLFTIMLLVLIFLSMIFLVYGEKIGYFIF
                     NLIGLDEIFIKIWDILRYTVGIITIIVIFTLLYKYTPNKKLTIKESAPGAIFATFAWF
                     LVSFLYSYYTNYYANYEVIYGSIAEIIVLMTWMYFSSWSIVIGYEVNSRLYFRKIRHE
                     MLK"
     gene            complement(34480..36177)
                     /locus_tag="KRL67_004039"
     CDS             complement(34480..36177)
                     /locus_tag="KRL67_004039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HBH1524808.1"
                     /translation="MDYTKTYEEWTKGSYFDEDTKLELEKIKNNEKEIEDRFYKDLEF
                     GTAGLRGIIEAGTNRINKYTVRRATFGLANYILENTTKEETSRGVVIAHDNRHKSRQF
                     CIESANTLAACGIKAYIFDSLRTTPELSFAVRSLNAIAGIVITASHNPPEYNGYKVYW
                     EDGAQVMPEIANAITEKVNSIHDYSTIPTLTGANKNLVVLLDESQDTKFIEAVKSQII
                     RKDLVKNVGKSFKIVYTPLCGTGNIPIRRALKEVGFENIIVVPEEENPDPNFAGLDYP
                     NPEEKKALNRGILLAKEKGADLVIATDPDCDRVGVAVKTTTGEYALLTGNQIGGMLTH
                     YIIEGLKENNKLKENPTIIKTIVTSEFGADIAKSNNVDVLNVLTGFKFIGEKIKLFEQ
                     NKNRSYVFGYEESYGYLVGTHARDKDGVVSSLLISEMAAFYYSKGMSLYDGLIELYKK
                     YGFFKEQTISLTLKGIEGVEKIKEIISYFRENQIDYINSIKVVDKKDYKNGIDNLPKS
                     DVLKYFLEDESWVAIRPSGTEPKLKFYIAVKGASDIEADKKIQGLKKYIDDMVEKLK"
     gene            complement(36243..36833)
                     /locus_tag="KRL67_004040"
     CDS             complement(36243..36833)
                     /locus_tag="KRL67_004040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HBH1524809.1"
                     /translation="MKIVCLGDSLTYGFGVSRSNSWTNIVNKETRLEIVNKGINGDTT
                     SGMLARFNEDVVKNSPDIVFIMGGTNDFIAGARNEIINSNIMAMVHQSYSKNIIPIIG
                     IPLKPDIPNVRADWSCFTDFNIVSRNLESYSYWIKKFCMTFNTNFVDFYSEYNKNMEL
                     EGYKKLYFDGLHPTKEGHRIMADIFINSINKYIEEL"
     gene            complement(36943..37740)
                     /locus_tag="KRL67_004041"
     CDS             complement(36943..37740)
                     /locus_tag="KRL67_004041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="G5 domain-containing protein"
                     /protein_id="HBH1524810.1"
                     /translation="MEKREKRIIISSLLSVSILMGLVSIYSILNKEDISLTVKGQEQK
                     VSSFKKTVEELLDEQGVKYNSEDKINPSLDTELKDDMKIKVVKVTKSKKEEIEKIPFD
                     TKHVNDSNLLKGKSKVYQEGQEGEKKLVYNLTYHDGKLVKKVLSKEVISKEPTTKIIK
                     YGTKEKVLIASRGANIRGGKHMKVVATAYAGDTITSTGTTPRWGVIAVDPRVIPYGTK
                     VYIPKLGMTFVAEDCGGAIKGNRIDIFMNSEGKASNWGRKSIDIYLH"
     gene            38096..38617
                     /locus_tag="KRL67_004042"
     CDS             38096..38617
                     /locus_tag="KRL67_004042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBH1524811.1"
                     /translation="MKFERLRTSENKLYYKAMELYKISFPFHEQRKSSLQAEIMKNKE
                     YQFNLIYDKNQFVGIILCWETDSFIYVEHFCIFPEMRNNRYGQRALELLNKKGKTVIL
                     EIDLPIDEISIYRKAFYERVKYKTNDYEHIHPPYHEKFNGHNLIVMSYPEKLSKMEYD
                     NFNQYLRNNVMSF"
     gene            complement(39039..40118)
                     /locus_tag="KRL67_004043"
     CDS             complement(39039..40118)
                     /locus_tag="KRL67_004043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HBH1524812.1"
                     /translation="MELLPLLADFFNVSIDKLLNYKIDLSEEEVMKIYKELESGFARI
                     EIDLSTEEPKEECRQDLESVKKLSNMYIEKYPKSYLLKLRICSLYQMYSYKFGKSELN
                     NRVKETTNILEDIVRNTDDIQIKETALIILSNAYCMLEDYEKAELYLNMIHKSIGDTS
                     VNLAMIYLKQNRLEEAEILLQNKLFSNVFNMSMDCKGIINVYKNQYKELKKKLENRNS
                     NKNAIERKMEYIKNKLLGYANLSLEIKKMLSEDKGAFFSMYMDYMELSLIFLFFNMKE
                     EAKKALYSLKEILEKYPIHESLDVSQMRFFDKVESKNLYTFNIYTNLLIMLNDNSYNE
                     LREEPIFKGVIEKILEMEKMLKNEE"
     gene            complement(40168..41745)
                     /locus_tag="KRL67_004044"
     CDS             complement(40168..41745)
                     /locus_tag="KRL67_004044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015520053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HBH1524813.1"
                     /translation="MRLSRDDENYGDSVSIETQRTILQQYAKEQGLHVVGEYVDDGWS
                     GTNFERPDFQRMMDDMEAGKVNCIVTKDLSRFGREHVMMDYYLEFLFPEKRVRYIAVA
                     ENEDTEKGLSDFVPFKNLFNEWFAKDTSRKVKAAFKAKFATGQRIGAYAPIGYRKHPE
                     IKNKLIIDEETRWIVEKIFDLAIHGRGAASITRILIAEKVPTPGFINFQRDGTFANIY
                     AGAPEEKSYAWTIAQVKSIMKDETYIGHTIHYRETNISFKNKRRVRKPQSEWVRVENT
                     QEPIISEQVFRQVQEQIANRRRKCKDGTTQIFSGLVKCADCGWSLSYGENRQNSKPYG
                     HYHCSKYGQGTRQCSMHYIRYDVLYAYVLSRLQYWSGLVQHDEERLLKQLLNATDKGQ
                     AAARKKQAAELKKAEKRKAEVDTLFARMYEDWAAGRITEYNFSMLSGKYQSEQAELDE
                     KIEQLQSAIATESQNAVDAEKWIALMKECVNPTELTAELLNMLIEKIVVHEAVKGEDG
                     SREQEVEIFYRFIGKID"
     gene            complement(41891..42085)
                     /locus_tag="KRL67_004045"
     CDS             complement(41891..42085)
                     /locus_tag="KRL67_004045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002578728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH1524814.1"
                     /translation="MAKSESDIFTPRTGQVIQAENGTQYFVCGNNRIKISEHFAAGGK
                     PLGDLIVDVVRHTAEKAAST"
     gene            complement(42158..43102)
                     /locus_tag="KRL67_004046"
     CDS             complement(42158..43102)
                     /locus_tag="KRL67_004046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002569775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid recombination protein"
                     /protein_id="HBH1524815.1"
                     /translation="MAQHAILRFEKHKGNPARPLEAHHERQKEQYASNPDIDTSRSKY
                     NFHIVKPEGRYYHFIQSRIEQAGCRTRKDSTRFVDTLITASPEFFKGKSPKEIQAFFQ
                     RAADFLIGRVGRENIVSAVVHMDEKTPHLHLTFVPLTKDNRLCAKEIIGNRANLTKWQ
                     DDFHAYMVEKYPDLERGESASKTGRKHIPTRIFKQAVSLSRQARAIEAALDGINPLNA
                     GKKKEEALSMLKKWFPQMENFSGQLKKYKVTINDLLAENEKLEARAKASEKGKMKDTM
                     ERAKLKSELDNLQRLVDRIPPDILAELKRQQRHTVKER"
     gene            complement(43287..43682)
                     /locus_tag="KRL67_004047"
     CDS             complement(43287..43682)
                     /locus_tag="KRL67_004047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009296947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HBH1524816.1"
                     /translation="MTNTIYIHQPEKAFSFTRLPNFLFEAPTFKPLSNEAKVLYAFIL
                     RRTELSRKNGWADDCGRIFLYYPICEVVDLLHCGRQKAVNTLRELQYAGLVEIQKQGC
                     GKPNRIFPKSYEAVPNTDFKKSRSGTPED"
     gene            complement(43794..44207)
                     /locus_tag="KRL67_004048"
     CDS             complement(43794..44207)
                     /locus_tag="KRL67_004048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002578725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich VLP domain-containing protein"
                     /protein_id="HBH1524817.1"
                     /translation="MRNNPYKDLPPLERRPDGSLYRMTPAQRKQAASLIRRECCCCED
                     GNCIVLDDGDTCTCPQTVSFSVCCKWFRWAVLPLDGTLEAEIFRDKDLKRCVVCGGVF
                     VPKSNRAKYCPGCAARVHRRQKTESERKRRSAVDS"
     gene            44519..45241
                     /locus_tag="KRL67_004049"
     CDS             44519..45241
                     /locus_tag="KRL67_004049"
                     /inference="COORDINATES: protein motif:HMM:NF012792.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000393259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBH1524818.1"
                     /translation="MNIIRLSKEHIEECADLFIDVFTKAPWNDTYNSKGQVISYFQNY
                     INNNYFVGYGLKDQNCMIAISVGMKKPWINGMEYYIDQFCVKTDLQGKGIGSYFLKLI
                     ENEIQTEKMEEVLIDDNMVFDIDNLKGFLNDTSSFGFIAKENNKIIGFAYCYTLLRPD
                     GKTMFYLHSIGMLPNYQDKGYGSKLLSFIKEYSKEIGCSEMFLITDKGNPRACHVYEK
                     LGGKNDYKDEIVYVYDYEKGDK"
     gene            45242..46681
                     /locus_tag="KRL67_004050"
                     /note="aph(2'')-Ia"
     CDS             45242..46681
                     /locus_tag="KRL67_004050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(2'')-Ia"
                     /protein_id="HBH1524819.1"
                     /translation="MNIVENEICIRTLIDDDFPLMLKWLTDERVLEFYGGRDKKYTLE
                     SLKKHYTEPWEDEVFRVIIEYNNVPIGYGQIYKMYDELYTDYHYPKTDEIVYGMDQFI
                     GEPNYWSKGIGTRYIKLIFEFLKKERNANAVILDPHKNNPRAIRAYQKSGFRIIEDLP
                     EHELHEGKKEDCYLMEYRYDDNATNVKAMKYLIEHYFDNFKVDSIEIIGSGYDSVAYL
                     VNNEYIFKTKFSTNKKKGYAKEKAIYNFLNTNLETNVKIPNIEYSYISDELSILGYKE
                     IKGTFLTPEIYSTMSEEEQNLLKRDIASFLRQMHGLDYTDISECTIDNKQNVLEEYIL
                     LRETIYNDLTDIEKDYIESFMERLNATTVFEGKKCLCHNDFSCNHLLLDGNNRLTGII
                     DFGDSGIIDEYCDFIYLLEDSEEEIGTNFGEDILRMYGNIDIEKAKEYQDIVEEYYPI
                     ETIVYGIKNIKQEFIENGRKEIYKRTYKD"
     gene            46898..47698
                     /locus_tag="KRL67_004051"
     CDS             46898..47698
                     /locus_tag="KRL67_004051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002606602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBH1524820.1"
                     /translation="MELSIQERLKDLRVERGLTLEQLAEQTHLSKSALGSYEGDNLKD
                     ISHYALIELAKFYEVTVDYLLGRSQTRNHPNADLADLHLSDDMIELLKSGLVDNSLLC
                     ELATHPDFPRLMADLEIYVNGVAGKQVQSANAIVDAVSATIMKQHNPGLTDPQLRQLI
                     AAHIDDDSFCRYVIQQDINKIALDLREAHKDDFFSVPEDNPLEDFLQTAEETTREDSD
                     PEQAAMMFICKRLKLNYKKLFEEEKKWLKRIAQKSDLLKNPKPQRGRK"
     gene            47819..48352
                     /locus_tag="KRL67_004052"
     CDS             47819..48352
                     /locus_tag="KRL67_004052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007061211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBH1524821.1"
                     /translation="MNTIKLRPIITVEEKNTFVSEIQEAFQISYVSEFGEFEKQVLPT
                     EDIEESFNAKGAEAYIAEIGGERIGGAVIVIDDKTGYNSLHLLYVKSSAQNAGNGFKI
                     WKAIEELHPETRIWETHTPYYDKRNIHFYVNRCGFKIVEFFNPKHKDPHQISESAGNI
                     PKENNYFFRFEKEMKSR"
BASE COUNT        15642 a         8679 c         6134 g        17951 t
ORIGIN      
        1 aatcctgcta ctaaattatt aaattgaaat cctataacct tataatattt tctaaataaa
       61 gtgattatat ttatatatat tgttgaaaaa tataagcctg ctataatcag caaagtgatt
      121 aaaattacat aaattttatt tatataggaa aagataaata atatacagca atttacaata
      181 gaaatagcta taagtagtaa aaatacataa atagccataa aagagataaa atcaattggt
      241 atactcttca aatttaaata atataaatct ttggcaaagt tcatattgat tattggcata
      301 tttctaaaaa tgaaattaaa tatatattta catataaata atgttgctat ctgacttatc
      361 aaaaaaccat atatcattgt tatcaaagct attgatttag atatacaaat cttaaattta
      421 ttttgaggta acatcataag tgtataaata ctttgattat ttgaaaagaa ctctcttttc
      481 cacataaaaa tatagtatat aaaaagtccc aacatcccaa agaacaatat actttggata
      541 aagccaagat tactaagtat ccctattcca taaaatatac ctccaaaatc tcctattgct
      601 aaattcataa taaatttaga atttttaaat ttattaactt cgaataaatt taaactaagt
      661 gtaattaatg aaaaaaatga tatataagct atataaaact tattgcttct tctcaaatct
      721 acatttatta gatatagaag tttattcatt cttatatacc tccctcatct tatctaatat
      781 agatttacct tctctttctc ttaattcttc tgcattaaat gaagttataa tctgcccttc
      841 atcaagaatt acgacctcat caactaaact ttctatctct actatctcat gagtagttat
      901 gataatagct tgattttcct ccatatattg cgctattact cctataaact cttccctctt
      961 aaacaaatca attcctgtaa atggttcatc aagaagtatg tactctgggt cttggcaaaa
     1021 tccaagtatt aattttactc ttgcaatgtt tccttttgac aagttatcaa ttatttcatc
     1081 ttcatccaat ttaaatatgt ccatcatttc ttttgatttt tctttattcc actttgaata
     1141 aaaaatttcc ataaattcaa atgtttcttt tattgttgta ttgctaaaat gagattgtac
     1201 atctggaaca aaagccaatt tattgtatac cttttcattt attttttctc catctatacg
     1261 aatctctcca ctatcaatgt ttataagtcc tgctattgct tttaacatag ttgatttacc
     1321 aacaccattt ataccaagta atgcagttat ttttcccttt tctatattaa aacttacatt
     1381 ttttaaagct tttatctttc ttattttaag cccttgcatc cttttatatg ttttgctgac
     1441 attctttatt tctatcatat ttactatcct ccacatccaa tatgcttata aataaacttt
     1501 ctaaactaag ttttctattt gttaaaaact aatacttatc ttctattatt ttcaaaactt
     1561 cttctttgct aacatttatt gctttcatat cttctataaa aacagatagt gattcattta
     1621 taagctctaa ttttaaattt tttaatatat tttcatcaac tgttacactg ctttgataat
     1681 ttttaaaagt atttattata ttcatatcac ccatctcctt atatgctctt tgaactgtat
     1741 ttaaatttac ttttagatta agtgccattt ctcttcttga tggtattgtc tctcctggtt
     1801 tcaactctcc tattactatt tgtcttttta tatactttac tatttgcaaa taaatgggag
     1861 ttgtattatc taattcaaaa ttcatgattc ctcctattct agtgtattaa tacgatagta
     1921 cacttacttt aaaaaaatac gcctttattt cagaccttat ctagtatcct attaagtgta
     1981 ttatacgatt aatacactta atttgtaaat acttatttta taaattgctt tattttaatt
     2041 tactaaaaaa tctaaagtaa agcttaaata ataactttac aaaatggagc atcaaatgag
     2101 aggatatata ttttatgtta tgttagactt gaaaacaagc aaacgagata ggattatatg
     2161 ttttcaacta atcaaaatct tatatgtatt aatgcttaaa aatctagtaa gaatatgaac
     2221 acacattagg aggtaaacaa atgagttata aaattgaaat caaaaatatc gaatcagtca
     2281 cagtggctac catgcgttat aatggaccta tgactgaggc aacaaaatat tttccaaatg
     2341 tatttaaagc tataaaagga aagtctaacg gggcaccatt cttttgctac tatgatgttg
     2401 accaaaaaac tggtgttgca gaaatggaat tatgtgttcc aactgctgag actccaaatc
     2461 gtatgggtat aactacaaaa gaatttccta aaacaaaagc tttatttttt actcacatag
     2521 gttcttatag taatttgcca aaaacgtatg aaatgatatt taaatatatt catgaaaata
     2581 atataaaaat acaaactcct tggagagaag tatttattaa aggaccaggt atgttgataa
     2641 aaggaaaccc agacaactat attacagaga taatatttcc acttaaagag gaggaataat
     2701 atatgttagc gattgaaata gacaatttag taaaagaata taaaaatggt gtgaaagctt
     2761 taaatggtct aagctttaat gtaaattctg gagaaatttt ttctttactt ggtcctaatg
     2821 gtgctggtaa atcttctctt atcaatattt tgactacttt ttacaagcct acatctggaa
     2881 atgtaacaat gtttggaaaa gatttagtag ataatccttc ttggatacgt actcagattg
     2941 cttgtgtagc acaacaaatc tctattgatg aacacttatc tcttatggaa aatatggtat
     3001 ttcaaagtaa aatgtataaa gtagaaccac agattgcaaa acaaagaata gactctttaa
     3061 ttgataagtt tgacttatct agttacttaa aatatcctac ttcatcatat tcaggtgggg
     3121 taaaacgtag attggacata gctatgaata tggtatcatc tcctaaaatt ctatttctag
     3181 atgaaccaac ggttggtatg gacgtagatt caagaaaatc tatgtgggat atgttattaa
     3241 aaattagaga tgagtatggc acaactatat ttctaactac tcattatctt gaagaagctg
     3301 aacaattaag tgataacatt tgtattatga agaatggaaa agatttagca caaggaacac
     3361 catcaagttt acgtagttat attagacaaa atatactacg tataactttc cacaatactg
     3421 aagatataaa aaaatataaa gatgcaatta aaagtactgg tttagtaaag tttatgaacg
     3481 tacgagaaaa ttcaattttt ataagtgtaa acgatagtag aactgccttt actttaataa
     3541 ataaatggct tttagaacat gatataaaat ttgatgcaat agaaattgta gagccaagtc
     3601 tcgaagatgt atttttagca cttacaagtt ctaaaaaaga cttaaaggag ggatgggaat
     3661 gttaaatatc ttatggcgta gtatgaagtg gcgttttaaa aatccaatct catttgtagt
     3721 tactatatta caaccttttc tttggcttgt gctttatagt tctattgcaa accaaactat
     3781 gaataatata aatatcaata attatactgc ttttatacta ccaggtatta ttgtacttgt
     3841 tgtgttttct tcttgtagta gtggtggtat tataaacttt ataatgaaga acagtggaag
     3901 tttctacaga gtcttaattg cacctatcag tagatattca atcgtccttg gtcaattatt
     3961 agaagctata ttagtttcct tcatagaagt agctattttg tgtatagtaa gtatattctt
     4021 ttcagtaagg atagaatctg gtattggtgg aatcctactt atgatagtgc tgatatttat
     4081 gacagccttc tttttatcaa gtctagctta ttctataagt ttattattgc caaatgagat
     4141 agtttatgaa acaattatga ctgcaattgt acttcctatt ttctttttaa gttcagctct
     4201 tttccctata gaaaatttat ctggaggatt aaaagtagca gtgatgctca atccatttac
     4261 ccatgttatt aacgctttac gtagcttaat atttggtgag actattctta ttggagatat
     4321 actgcctgtt atcctactat ttttaatatt gtgctgtgca agtttttcac ttgctatgtg
     4381 gagattaaaa aaagaaatgg taagctaagt tttaaaccta ttaataacaa ataataaaag
     4441 tggactctat tatatagaat ccacttttat catttatttt aatatttcta aaaccgaatt
     4501 taacacagca tgaccattac aagtaccata gtttaccata tctattatag ctactggagc
     4561 tctatctcca actatagact ttacatcatc aagcataaat ctaagttgtg gtccaagaag
     4621 taaaatatca aagttatcaa gctccgattt tatatttcct tctccaactg cccatatttt
     4681 acagtctata tttctttttt ttgcatcctt ctccatttta tttaccaata aggtagaaga
     4741 catgcctcca acacacacta acaatatcct aatcatttat agctcctttt taaccaaatc
     4801 tacatatatt ttgattaaaa agtatatgta gaaaagttta tcatttataa ttacttaaat
     4861 tttactctta gattatatca aatttttcca tttttttcac taccagagcc aattttatat
     4921 ttttaatgtc cacttttcca cttctttaga gatatcttca gcttttaata tagctgatat
     4981 tatagcatac ccgtcacttt ctaaacattt aagtttatct atattgtcta aatttatacc
     5041 tccaattagt acaaatggta tgtctacttt ttctttaatt tctttaacag tttcaaacga
     5101 agctgatttt gcatctaatt tagtagatgt tgtgaatata gaccctatac ctaaatagtc
     5161 tgcgccatca ttttttgcac aaatagcttc ttctaaagtt cttgctgata ctcctagaat
     5221 tttattgttt cctataagct ttctaacctc tctcacctca atgtcactct gtcctacgtg
     5281 tactccatct gcatcacaaa taagtgctat atccactcta tcatttatta taaacttaac
     5341 accatatctt ttagttattt ctctaagttt catagctttt cttaaaaact ctcttccaga
     5401 agcatttttt tctctaagtt gtacagttgt tattccagaa gatattgcat cttcaagaca
     5461 tttataaaag tctctacctt taagcatttc agagtcagtt actagataca actttaaaca
     5521 tttttttaaa aattctttat caatcatttt aagtctccct tactctttat taatttaaaa
     5581 attcaatatt agaattttta cttaaagtat ctatatccat ttgataaata gcattcataa
     5641 gattttcttt aaatgtacct attggcggat tatttaaatt agccaattct ccacttaaag
     5701 acattgtaag aacacccatt gttgctgctt ctaatatatt ctcagtaaca gccataaaac
     5761 ttgcaatcaa gctagcagtc atacatccag ttcctgtaat tccttttaat ttatcagttc
     5821 cattgtttat tttatatgta cttttaccat tagttattat atctattttt ccagttgcta
     5881 caactacaca ttcaagcttt tcagcaatct ttcttattat ctcagtagaa tcttcttctt
     5941 catcaaatga atctacacct ttacccctta cattaaagcc acctataaac tttatttctg
     6001 acacattccc tttaacaaca ctaaatttga cttcatttaa aagtctactt gtaagttctg
     6061 ctctcgcttt gcttgcaaat actccaactg ggtccaaaac aacaggttta ttgtacttat
     6121 ttgctgcttt tccagctaat aggaataaat ccaacatatt tgagttcata gttcctatat
     6181 ttattactac agagtttgct acacttacca tttcttctac ttcctcatat gaaaaactca
     6241 taagaggact tgctcctaca gctaaagtta cattagcaca atcatttata gttacattat
     6301 tagtataatg aattaccaat ggatttaatt tttttacatc tttaattaaa ttatacatat
     6361 tctacctcca atattttttt attactcaaa tttataaaaa tgatgtactg gtcctactcc
     6421 atgacctata tcaaagcttc tttttattgc ttctgttata taaattttac ttagatttac
     6481 tgcttctgtt atttcatatc ctaatgctag ataagatgtt attgcagaag ataatgtgca
     6541 ccctgttcca tgtgtatttt ttttatcaat tctttcactt ttataaactt taaatatatt
     6601 tttaccaact aaaatatcca ctgcttctcc atctaaatga cctcctttca taagtacaaa
     6661 tttaggtcca agttgtagta tttcttttcc tactctcttc atatcatcta cattatgtat
     6721 ttttattcct gtgatttctt ctgcctctgg tatatttggt gtaattatgt atgcaagtgg
     6781 tatcaaatgc ttaattaagt tttcttttgc ctctggcttt aatagataat atccactttt
     6841 agatatcatt actgggtcca caaccaaata ttttggattg tatttcttta gattctcaac
     6901 tatttcaagt ataatctcag ggcttgaaac cattcctatt tttacagctc ttggaggtat
     6961 atcttcaaag actgcctcta tttgtttttt tattattttc ttatttaaat cctctacatc
     7021 aaaaactcct ttagtatttt gtgctgttac agctgttatc acactcatac cataagtacc
     7081 aattgcacta aacgttttca aatcagcctg aatcccagct cctccagatg aatcagagcc
     7141 agctattgta agtgttggta ttttataatt actcattgtt gcctcctaaa gttttattta
     7201 aataacttaa ttaaaaaagc tatacctaca ataggtatag cttagaaata gcatatatca
     7261 caaataatac atacttgaat ttttactaag ctttcctacg ctggtattat ccagatcagg
     7321 tgtaagggtc ggtatacctc tcagctcttg gctcccctag ctattttaat ttactatatt
     7381 tataacttca ttatattaca atagtattta ttgtcaagtt tttacattat accaactcat
     7441 tataatgtta ctataaatat ttatgttaat tgttctattt cattaccaat aatataatcc
     7501 tctttattat caattaaatt tataagtata cttggatttt ttattataga caatttttta
     7561 tttctagtta atttttttat atagtattct aacttcatag cattgctttt tgtgttggtt
     7621 acaaaataaa cttctagttt tttaaacccc tttgctcttg tgtattttga attaattcct
     7681 aacttatgtt cattcattct tctaactata ttactggtat atccagtgta tagagaatca
     7741 tctttacatc ttataatata tgtaaaatac atcttaaatc acatcctaac attaaatata
     7801 taaacattat attatagaat taaaaaaaat ggtgtgtatt ttgaatatgc aatatccaaa
     7861 gagttaatta taatatatgt ataaacatgc tctacatgaa tctaatgcta aattttgaca
     7921 taataataac atctttattt ctataataaa aaattcacaa attataaaaa ataaaatttt
     7981 caaaaaaatt ataattttca aaaaaataaa ttgacacata taaaaaaatt atgataccct
     8041 aataaaaagg gggcaatgat tatggaaaac aatttaaata cagctaaaag aaataatact
     8101 gaattatcac aaaatgactc acttaaggtc aacttacaga taggtgtaga ctttacactt
     8161 ttatcaaaat cacttaaagc cttttataaa aaagaaaaag atttttattg cattgctctt
     8221 gcgccatgca atgtatctag tgaaaaagca aatagaaaaa taagtattta cgatatgata
     8281 aaagaaatta atattttaat tgaaagtata actgataaaa atgaactttt aagtgaagaa
     8341 ctctttacta ataatttatc agaattttgt gacaaatctt tagatgagat ttatatagac
     8401 ttaaaacaag cgtatttata tattaaaaag aatacagata gtcttaacat agaggactca
     8461 attgaatatg tatttgatgt agatataagc aatgatataa aagcaaaaga aaatccacta
     8521 attaatttta attcactttc tttagtagta tggagtggcg aaacaaaaca tattttacaa
     8581 caaatgaata ttttagattt agaagatatt cactcttatt ctatggaaaa taatacaaat
     8641 ataactatct atgaaaatga tactgttcaa tataaatttg atgaagaatc tgaagatata
     8701 aaagacagta gcatagatat aattgaacaa atgcttatac ttcctgatga cttgaattat
     8761 aataaagatg aagttgaaaa tatgaaaagc agacttgcaa aaataaatat taaatattta
     8821 cagacactta aagaaaagga tataaaaata aaattaataa attcaaatct aactgatgaa
     8881 ccagaattta gtgatttaaa atatcaatta cctccatgtt gggtaagatc aggcaagact
     8941 tggaaagatg tacctggtat atatagaaat aattctatag ttgctaaaat aggttatagt
     9001 aaccctagct atgcaaatgt acattcatca aaaaatttag aattacatga aactgcacat
     9061 gctatagata aaaacgtttt aaataaaaaa tcaaattctg aagaatttat agaagtattt
     9121 gctcaggaaa gatataagct ttatgaccct aaacaagttg cacatgcata tataagcaag
     9181 tttatagaag aattttttgc agaatcattt gtacattatt atcttgatga agattcaaaa
     9241 aatactttaa aggaaaattg tcctctgaca tatgattttc ttgaaaaact tgaactaaac
     9301 tattaagtgc gctaatatcc aataatacat ttaactatcc tcttttcatc aacaataacc
     9361 atataataat ttatactttg aataaatcac tatctataaa ggatttctta atatttatgt
     9421 tataatattt ataatggtta tgttttaaaa tagtttatgt agcccataat ttaaataaat
     9481 aataaaagaa ggatgaaaag ttatgaagtt aaatagaaat gacagttgtt ggtgtggaag
     9541 tactaaaaaa tataaaaagt gtcacatgga tatggataac aaaatagatt catttgctaa
     9601 gttaggtcat atagtaccaa ctcatgatat tataaaaaat gaaagtcaaa ttgaggaaat
     9661 aaaaaaaagt gctgtaataa acacaaaaat tttggattat atttcagata aaatcgaaat
     9721 tggaatgtct acagaagata taaataagtt ggttcatgac tttacactac aaaatggagc
     9781 tattcctgct ccactagact ataatggatt tcctaaaagt gtatgtacat cagtaaataa
     9841 tgaagtttgt catggtattc caagtaaaga tattatatta aaagatggag atattataaa
     9901 tgtagatgtt tctactattt ttaatggata ctattcagac gcttcaagaa tgtttatgat
     9961 aggtaatgtt aacgaaaatg caaaaaaatt agttcaagtt acaaaggaat gtgtccaaaa
    10021 aggtcttgaa gttgccaaac catggggatt cttaggagat ataggtgatg ctatcaactc
    10081 ttatgcaaag aaaaatggat actctgttgt tagagaaata ggtggtcatg gagttggatt
    10141 aagctttcat gaagaccctt ttgtaagtta tgtatctaaa aaagggactg agatgctatt
    10201 ggttcctggg atgatattca caatagaacc tatggtaaat gaaggaactc acgaaatttt
    10261 cgttgatgaa gaaaataatt ggactattta tacaaaagat ggtaaattat ctgctcaatg
    10321 ggaatgtatg gttttagtta cagaaacagg tgttgaaata ctaactgaat aaaagacaaa
    10381 tgcctataaa aattgaaaat tcaattttta taggcattta ttttattaat gcacatctat
    10441 tgcatcaact ggacagcttt ctctagcttc tgctgctgat tcttcagcat cacttggtac
    10501 ttcatcaact ataacatgtg atttcccatc atccttcata tcaaatactt ctggacatat
    10561 tgatggacat aatccacaac ctatacaagt atcttgatta acatttgctt tcataataaa
    10621 atcctcctag acgctacaaa tttaaaatct tgtttatcaa agattgatga actaaaactg
    10681 taacattcag ttctttttta ttatgtccta aaatttgaaa aaaatacctt ttaaaaataa
    10741 tttttataaa gtttatttta tagttatgct aaagttatat ataatataca ttatctaatt
    10801 catcatactc atagcctact tcttttttat tcttttttac aggttctaag tttcttattt
    10861 ttactatttt tgctggtata ccaacaactg ttgcaccatt aggtacatcc ttaacgacaa
    10921 cagaatttgc accaattttt gaatttgaac ctatatttaa aggtcctagt atttttgttc
    10981 cagcaccaat taatacatca tcaccaacag ttggatgtct ctttccagta tcttttcctg
    11041 tagctcctaa agtagctcct tgatatattg tcaccctatt acctacttca gctgtctctc
    11101 caattacaac acccattcca tggtctatca atataccttc ccccatttta gcaccaggat
    11161 gtatttctat accagtcata aatctggata tctgggatat taatcttgct gtaaaaagct
    11221 tcttcttctt ataaagagca tgagccattc tatgcattat cattgcatga actgatggat
    11281 aaagtaaaaa aacttctatc ttagacctag ctgctggatc attcttcatt atatattcaa
    11341 tatctttatt tattttctta aacaaagtat tcactccctc aaataaaatt attaaaatat
    11401 tcccatagac atatacttat caactccatc tggagctact gtaactatct ttttatctgg
    11461 atactttttt gccatctcta tagcaccaaa tatgtttgct ccagaagata tacctactaa
    11521 aattccctct tttgctgcaa gtaatttaac tgtatcaaat gcatcttcat catctactaa
    11581 tattacttca tcaactacac tagaatcata attttcagga acaaagttag cacctattcc
    11641 ttgtatttta tgaggtccag tcttgttttc tgatatagct ggagattttt ttggttcaag
    11701 tgcaattact cttatatttg gattttgttc ttttaaatat ctcgcagttc ctattaaagt
    11761 accacctgtt ccaactccac atacgaatat atctaaatct ttgacatcat tcataatttc
    11821 aactgctgtt gttttataat gtgcatttgg attatcttta ttttcaaatt gttttaagct
    11881 tttatagttt ggattttctt ttaaaagttc atttgctttt tctatggaac ctgccattcc
    11941 agctttacca tctgtaagca ccaaatttgc cccataagcc ttcattaaat ctcttctctc
    12001 aacgctcatc gtttcgggca ttacgattac tacttcatat ccttttaatt ttcctatcat
    12061 agataatgca ataccagtgt ttccactagt tgcttcaact agtacatctc ccttttttaa
    12121 ttctcctctt ttctctaagc cctctaacat ttcataaaca gctctatctt ttatacttcc
    12181 tgctggattt actttttcta attttacata tatgttagaa cacccattag tacagcctaa
    12241 attattcagc cttactacag gtgtgtttcc tatcaactct aatgcgttat tatataacat
    12301 actatttcct cctatatgtc caaatctatt atcaaattga taacgataaa tatattatag
    12361 tattatcatt ttgataatgc aacctttttt tggattttat acttacaatc accttattcc
    12421 aaatattagt aatattcaaa taaaaaagtg aaaatcatta tcgattttca ctctttaaaa
    12481 tttaatcttt aaattaaatc ttgcgtaata ttttgttact aaaattctac aaaatactat
    12541 ataagaccta atatttttgc gacctctaca ttaaccttta gcacattaac tctatcttca
    12601 cctaaaactc caaaaagttt tttggaagta ttatcatcta caattttttg taatttactt
    12661 tctgatattc ctgatgcttt tgatacagca ggtatctgaa tttttgcaga agctggactt
    12721 atattagggt caagccctga acctgatgct gtaagcaaat ctgttggtat atcctctttt
    12781 ttgacagttg gattatcctt taaaaactgt tttatatctt tttgtactct atcatgaagt
    12841 tctggatttg tagctccata attaaaagaa cctgatgata cacctccata agatgtattt
    12901 ccatctttat ctggtactaa atcttctttt gtataagtat tatagtttac tgatgatact
    12961 cttcccttaa aaaatctcgc atctgtaaaa ctctgaccaa taagttctga acctactttt
    13021 acaccattta cttctatcat acttccatta gctttatctt taaatgcaac ttgacttaca
    13081 cttgtcaaaa caagtggata tacaagtcca caaacaacta acaatacaat acttacaagt
    13141 aatgctggct ttaatgtttt catttttata tcctccattt attaacctaa attaagaacc
    13201 attactaaag gtgtaatcaa taaatcaatt aattttattc ctaaaaatgg tactataact
    13261 ccaccaagac caaatataag catatttctc attaacatag cttgtgattt cataggtcta
    13321 tattttacgc ccttcattgc aagaggtatt aaacatggga taattatagc attaaatatt
    13381 aaagctgata atatcgcact aaatgatgta gaaagtttca ttacatttaa tatctccatt
    13441 tgaggtattg caagagtaaa tatagctggt ataatcgcaa aatattttgc aacatcattt
    13501 gctatactaa atgttgtcaa agccccacga gttattaaaa gctgttttcc tatttcaaca
    13561 acttccaata tcttagttgg gtctgaatct aaatccacca tatttgctgc ttcttttgca
    13621 gatgttgtac cactattcat tgcaagacct acatctgcct gagcaagtgc aggagcatca
    13681 tttgttccat cacctgtcat tgcaactatt ttaccctcta attgttcttt ttttattgca
    13741 tctattttat cttctggttt acattctgca ataaatccat ctactccagc ttctcttgca
    13801 attgtagctg ctgtcaaggg attatcccct gtacacatta ttgtctttat tcctatctct
    13861 cgaagtctag caaatctttc tacaagccct gattttactg tatcttttaa gtagataatt
    13921 ccatatatct cattatctac acacactact aaaggtgtcc ctcctagttt agctactcga
    13981 tttacatttt cttctaaatc ttctggcact gtaccaccag attcttttac tctttttatt
    14041 atgctatcat aagcaccttt tcttatagct ctaccatcag ctaagttaat accactcatt
    14101 ctagtttgtg cagaaaattc tacaaactca gcttcctctg cctcttttgt cttacctgtt
    14161 gaacctaatt tcatacctaa atcaactata gatttccctt ctggtgtatc atcttttaat
    14221 gaacacataa cagaataatt tattaagtct tttgaatctg catttccaac tgctataaat
    14281 tctgatgcaa gtctattccc ataagtaatc gtacctgtct tatccaaaat catagtatca
    14341 acgtctccac aagcttctac tgcttttcct gacattgcaa ttacattaaa tctagttact
    14401 ctatccatac ctgcaatacc aatagctgac aacaatccac ctatagtagt tggtattaaa
    14461 cataccaaaa gagctatcat agtagaaatt ggaatcttta cacctgcata gtttgccatt
    14521 ggataaagag atattaaaac aaccataaat ataagtgtta accctactaa caaagtattt
    14581 aaagcaatct catttggcgt tttttgtctt gaagcacctt ctacaagagc tatcatttta
    14641 tctaagaacg attctcctgg ttttgttgtt attcttatct ttaaccagtc acttacaact
    14701 gtagtaccac ctgtaacaga tgcgaaatct ccacctgatt ctctcatgac aggtgcagat
    14761 tctccagtta ttgctgactc atccacagaa gctataccct ctataacttc tccatcattt
    14821 gggataactt ctccagcatt tactaaaaca atatcaccaa gttttagctc acttgcactt
    14881 atcatttttt catttcctaa cgtatctata acccttgcta ctgtatcttt tctagttttc
    14941 ttaagtgtat ctgcttgagc tttacctctt ccttcagcta cagcttcagc aaaatttgca
    15001 aaaaggactg ttataaataa gattattgtt acaatcccat tataagctct taagttattg
    15061 ccttcatcac caaacaaact aggtactatt gtaagtatca atgttactat aaatccaatt
    15121 tctacaacaa acataactgg atttttcatc atatatttag gatttaattt tttaaaagct
    15181 tctataatgg aagttttgaa aatatctttt gtaataaact ttgttttttt agtttccata
    15241 aaactttact ccctcccaat tttttagtgc cacaacacta gatgctccga aataggtcca
    15301 agcgctaagg ctgggaaaaa tgttaaagca ccaacaatta aaacaacaag tactaaaact
    15361 attgtaaatg taaaattatc cgttctaagt gttcctatac tttcatttac agctttttta
    15421 gatgctaaca aacttgatat tgctagcagt actataatag acacatatct tccaaagaac
    15481 ataactatac ctgttgtcat attccaaaac atagtgttat ctcctagacc ttcaaatcct
    15541 gaaccattat tggctgctga agatgcaaac tcatacaata cctgtgataa tccatggaat
    15601 ccaggatttg aaattccttc aagccctgct ggtattgcta ctgaaagacc agagaacatt
    15661 agtattaaaa gtggatgtaa tataataagt aaagcaataa gcttcatttc ttttccttcg
    15721 attttcttag ttaaaaactc tggtgttctt cctaccatta agccacataa gaatactgct
    15781 attatagcgt acatcaacat attcatcaga cctacgcctt taccaccaaa tactacgttt
    15841 agcatcatat ttaaaagtgg tactaatcct cctaaaggag tcagtgtatc atgcatatta
    15901 ttaactgtac ctgtcgtaaa ggaagtagtc actgttgtga ataatgatga ttgtgctata
    15961 ccaaatctaa cttccttacc ttccatactt cccatacttt gattaagccc taattgtgat
    16021 aaaatagggt ttcccgcact ttctgcttta aaacaaacta ctagacctat taaaaatatg
    16081 attgacattg cgccaaacac aacccaacct tgttttttat tctttatcat gtgaccaaat
    16141 gcaacaacac aagctcctgg taatatcatc atagataata tttctactat atttgatatt
    16201 attgttggat tttcaaatgg gtgtgatgaa tttgaaccaa agaatccacc accatttgtt
    16261 cctaaatgtt ttattgattc aagagccgcc acaggacctc tagctatatc ttgaagcttt
    16321 ccttctatag tagtcactgt ttctgtccca gcaaaagttt gtggtactcc ttgtgtaact
    16381 aatataagac caataattaa agctcctggc aaaagtactc ttgttgttat cctaattaaa
    16441 tctacataaa aatttcccaa tgttttcttt ttaccgacca gacctcttac aaatgccata
    16501 gcagccgcat atcctgttgc tgctgatgta aacatcatat atattatcac tgtcatctga
    16561 ctaaagtatg aaagtcctga ttcacctgaa taatgttgaa gatttgtatt tgtcataaaa
    16621 cttattattg tattaaatga tagtccttgt tccatacttt taattccact tggattaaaa
    16681 atatgcatac tttgagttct aagtatgata tatcctataa ataccataac tgcatttgta
    16741 aataacaaag ctaaggcata ctgtttccag ttcatttctt ttttcttatc tatactacat
    16801 accttatata taaaattgtc tactttatca aatagtctat ctccaaatgt tttttgatta
    16861 gtagctatat gatataaata cttgcccata ggtattacta aaatcataaa aatcgctaac
    16921 actattacta tctgcaccat aaaaattgtt ccccccaatt caacttactt attagcctta
    16981 tactgtttaa aatttttcag gattaataag cgcatatacc aaatatatta ttaacattat
    17041 aattacaact gccaaaaacc acataggtaa tccctccata cattacatta ttttttttca
    17101 gtttgtttcc cacaccaatc agcaagtaac ttcatggaaa caaatcctat gaataacaat
    17161 gctatcataa ctatatccaa cattttagct cctccattta ctttaatctc ttttacaaat
    17221 tttagttttg tattgctact tcatttatct tatcatttta ggtataaaga taggattaag
    17281 aaacatccat taatgattag tatttcataa atataaaatt gagtttttaa aacgcacatc
    17341 tagcaatcgt attcccagtt attccaacat aattctatat ttgagatttg caaaaaccta
    17401 gatgtgatta tactttctat aaagatttag acacaaaaaa ggtggcaaat ctgccacccc
    17461 taataaaatc taaagttatt taatttatat taacattctg gaactatatc attacttata
    17521 tccatatcca aacttttacc atgataataa ataaaatcaa ttaccttacc actaattgta
    17581 acagcaatca aagaataaaa gatatcaaac gctgatagat aaaataatga taacaacaat
    17641 acaataacat ctgttgctaa ataaacttgc cctatgttca aagatgtagt cttagaaatt
    17701 actaatgcta aagcatcgtc tcctcctgat gcacaacctg cttttactac tatacctaca
    17761 ccagttccaa cagttacacc tgccaaaata gttgataaaa gcatattttg tgattgtggt
    17821 actagaggtc ctatagcttc aaataagtca tataacactg aaaaagttat agaagctact
    17881 attgaatata taaggaattt tttaccgaaa aatttatatc ctactaaaag tagtaaacca
    17941 tctattacaa catttgctat tgctggttgt atattgaata aattttttaa cagtagtaaa
    18001 atacctaaca ctccaccttc tgttatgttg tttaaataat aaaaattata aactccaaat
    18061 gctaatattg ctgaacctaa aaatataagt ccaatccttt ctaaaaataa ccgcctggcc
    18121 atacatatcc cttccttttt ctgaatttat atatattata tacaaaagaa ttggctttgt
    18181 ataccacact tttcaaatct ttaattatac aaaatttcta tatttttttc aacaattttt
    18241 ttatgttttt ttatgttttt ttaataccat acctacacta cagtaattcc aatactttag
    18301 acgttttcct tttttccata agtttatatt agtttgactt ataatttagt ataattaagt
    18361 ctttgtttat attaattttt tatttgttta gaaatactat tcaataattt atcattaaat
    18421 taaaaaagag ttactttata tagtttctaa agtaactctg aaaatcttat ttattatcta
    18481 tgacaattta ccattcaccc ttgaactctg aaatattatc cttattaact tcaacttgtt
    18541 taatttgtgt aacaggttct attttttcac ctttaaggaa tttagatgcc ttttctataa
    18601 ctaatccacc ttctttcaca gcagacatat aagttgtacc atctattaac ccatcagtta
    18661 tagcttttaa tccatcttta cttccaccca aacctataac tttaacttta tccttaatac
    18721 cagcatcttt taaagcttga cctgcacctg tagcagagtt gtcatcttga catattataa
    18781 cattaaaatc agttttcttt tgaattaaat cttctgttac ttttaaagct tgctctttct
    18841 cccagtttga agtttgatta taaactattt ctatattact atcttttaat tctttttcaa
    18901 gacctttttt tcttaatact tgtggagtag ttcctggtac tccttctatt aatacagctt
    18961 ttccaccagc ttctcctaat aagtttttag caatttttcc agttaatgct cctgtctctt
    19021 cctcattttg tcctatatat gtaaccaaac ctttaaattc tccagttggg ttacttccta
    19081 tagaatttgt aaatgctatt ataggaacgt ctgaatcatt ggcagcttta actattctct
    19141 cacttacagc agaatcaact ccacataatg caatcatgtc taccccttta gccataaaat
    19201 cttcagcttg tgataattgt ttactagcat cccattgagc atctgcatat tccacactta
    19261 cacctaattc tttagatttt gcatctatac cttgtttagt tgctacaaag taacctgttg
    19321 aaccaggaag caatactgct attttctttt ctccctcttt tcctttacta ccattgtttg
    19381 aacatccaac taagctaaat atcataataa atgatattaa taatgctaaa tgtttcatta
    19441 actttttcat aaaaaaattc cccctcttat tttttatctt gaagcaagct ttttcttgct
    19501 ataagaatca aatccaactg ctgctaaaat tattaatcct ttaaatatgt actggaagta
    19561 tacatctatt ccaagaagat taaaactgtt gtttataaca ctcataagta atactcctat
    19621 aacggtttta agtacagaac cagaacctcc tgccactgat gtacctccta ttactacaga
    19681 tgcaattgca tccatatcat atccttcacc tgcaatcgca gttgcagtcc caagtcttcc
    19741 tgttagtacg attccagcta ttcctgcaag tacagcatta ataataaata ctttcacttt
    19801 gtgagaatct acatttatac cattaagttt acttgcttct tgattccctc ctacagagta
    19861 aacatatcta ccaaacttag ttttattaag tacaataaac gaagctaaca ccactataaa
    19921 gaaaattact actggtattg gtatattaaa caaatatcca tttccaatgt aatcaagtat
    19981 tggtatattt tcagtaattg gatatccgcc tgtaagcatc aacagcaatc ctcttacaat
    20041 agtgaccata gccatagtag ctattaatga tggtacattt atctttgcaa ctactatacc
    20101 attgattaat cctattactg cacaaattgc tatacctaag agcatggatg caaacaaatt
    20161 agttccacca gtcgcaaatt tcattgttat agcacctgca agagctgata ctgctccaac
    20221 tgatatatca aagtttccag atattataac aaaagtcata cctgctgaaa ttattccaat
    20281 tatagaagat tgtcgtaaaa gattaagtat attttgttta cctaaaaatg atggagttgc
    20341 aatacttata gctatacata acgctaaaaa acctagtaat actccataat ctcttaaatt
    20401 taacttctca aaagctttct tcatctcagt cctccatttt tcatttttta tacattaaat
    20461 atactgttca ttatgtcttg aacctgtata ttctcatttt ttaaatcatc tacaatagaa
    20521 ccttctctag ttactaaaat tctatcactt actcttatta cttcttctaa atcagaagat
    20581 atgaatatta ctcctattcc tttacttgaa aattctttga ttgtattgaa gatatcttct
    20641 ttcgcagcaa tatctattcc ctttgtcggc tcatcatata ttattaaatc taaatctttc
    20701 tctaaccact tagaaactac tactttttgt tgatttccac cactaagctc ttttacttct
    20761 tgctttacat cactaacttt tattcctaat tccttaacgc cttttttaga aaactcaact
    20821 tctttatttc tatctataaa caagaatttc ttaaatttat caatttgtac tgatgatgag
    20881 tttgtgtata tagcttgttc taaaattaat cctagatttt ttctatcttc aggaatcaat
    20941 cctattttat tttttatagc caccttagga gaagttatgt ctactttttt accattgata
    21001 aaaatgtctc cactgtcttt attatctata ccaaatatca aatttataat ttcagttctt
    21061 cttgaaccta caagacctgc aagacctaca acttctcctc tataaacttt aaatgaaacg
    21121 ttttttattt tgttagacca gcttatattc tttacttcta gtactggaag ctcttcatac
    21181 tttgcaaatg tataatcctt cttttcaaca agtttgtgat tatcgtttcc tgtcataagt
    21241 tgagttatct ttaatttagt taaatcctct acatcataag ttccaatcat aattccattt
    21301 ttcattatac ttgcactatc acatacttta aaaacttcct ctaatatgtg agaaatatag
    21361 actattgttt tacctttaga ctttagtcta cttattattt taaatagccc ctctttttca
    21421 ttattagtaa gtgatgttgt aggttcatcc attatgataa tttcagaatt acatgaaaca
    21481 gctttcatta tctcaaccat ttgttggttt gctacactta aattagaaac tattatatgt
    21541 gggtctatat taaaatcaaa atcctcacaa attttcatat attcttcaag catgtctttc
    21601 ttgtttaaaa acacacttcc ttttaaaagt tctctaccaa gaaaaacatt ttcaagtact
    21661 gtaagtgttg gtatcaagct taattcttga taaattatac taatcccatt ttctttagaa
    21721 tgatgtggat ttttaaacat tatttcatta ccagccaaaa tcactttacc tttagtttga
    21781 gaatgtactc cacctaaaat tttaataagt gtagattttc ctgctccatt agctcccaaa
    21841 agagctctta cttcaccctt ttctagctca aaatttatcc cttttaaaac atctaccttt
    21901 ccaaaacttt tatatatttg ttgcatttcc aatatcttac tcactatatc acctcactaa
    21961 ttccgaaacc attttataat ttcatcaata ttttcttttg gtgctgataa caaatcccaa
    22021 gataaaacat atccttttac tgttgtctta tttatatttg aaatattttc acttatgtat
    22081 ttaggtgtaa caggagctat aatttccttt ttatttattt caactcctac atatatatct
    22141 ttattaagac ttcttgtcat aaaatcgatt tcctctagta caaattctat aggatataca
    22201 tctccttcat actctttaat tcctaaaatc tctaagaaat ttctttttgc aatctcttta
    22261 ttcttatata tggattcatc aataaacctt tcaaattcaa atggcaaacc agctggagct
    22321 tttgtagttc tatacatcat tggttttata aaatctgcta cttcttgtaa tttttctaaa
    22381 tcttgacctg taaagtaaga aataaaagga gaaaaagtat ctatacctat tttcaatcct
    22441 ttacttttaa aataactact tatctctaac agtgccttgt atatattttg attttttgtt
    22501 tcaaaaaaat cttcccatag tttattttta aatttgtatt ttccattaat gtattttgtt
    22561 ataccaaatg gagtatcttc attatctgta actcccttag acatattatt catttctatc
    22621 attaatgact ttttatcaat attatttttt tcatatatat ttaaacaatc tttacaaaaa
    22681 caattaaaaa caccatttag tttattcgaa aaagaaccat accttatttt atctaaaaaa
    22741 attccatcaa atttaatatc actaaaataa gtactatata ttttctttat attttttata
    22801 ttttccttat tatttgggca ataaaattca aaattttcat cctcttgtaa agcatagccc
    22861 ttgacttcct tccccgtata atcaattagt aaattacttt tttcaagtaa tcctatttct
    22921 gaaaaacatg gcaaccaaag atatagttca acattatatt tatttaatag ttttttagtt
    22981 tctacatata attcttcatc tacactccag cccatgataa ccttttctat atcaatacag
    23041 tccagtaatt ctagtagctt acattttata tcattaaact ttatttcttt agtcttaaaa
    23101 cctccagtaa aaatttgtac tattaccttt tttctatcca tgcttgaccc tcctattggc
    23161 taatgccata gcaagtctta tccccccata tactggctga ttttcttgag atataaattt
    23221 taaattttta tctttatttt ctatttttaa attattcatt aacatttcag catatacagt
    23281 tttatttaat gtgccaccag acaaaactat atttatttcg cttttatccc ttttcattgg
    23341 attagatatt ttactcaata ttacttgagc taatttagac agttcagttg atgattctct
    23401 taatatttca atagccaatt tatcaccttg tttggcacaa tcaattacta atgaggctaa
    23461 gtttgctatt tcaacattat tgtttatatc agtaatcata tatgcaagtt gttcaaaact
    23521 atttgctcca aagaaatcta agacacaatt aaataaatca ctatactcat gacattcatc
    23581 acaataaagt aatactttct ttaataattt tctccctata tcatatccag aacccaaatc
    23641 actaaaatta tacccccatc caccaactct atatatttct ccatcttctt taattcctaa
    23701 tataattgaa cctgtcccag caacaattac taaaccaggg gaatcagctt gtgcataaaa
    23761 agctaaaact gcatcatttg ccagatatat tttttctttg ttaatcccaa tttttaatat
    23821 ttcatccatt ataatcttat aatcattagg agaatcacaa ccagatatac caaatacagt
    23881 gtactcaata tcgtcataat cgatatataa atcttttaat atttttttaa atccattatt
    23941 aatattttca tatgtttgtt taattccaac tgacttataa ttagttgaac cagtggtatg
    24001 ttctttaagt atgtttccat ctaagtcact tacacaaaat ttagtttttg ttcctcctcc
    24061 atctattgaa accaaatatt tcacattaat atcctccttt tttattagat aaggaatatt
    24121 gatagtcagt ttcttttaaa cacatactaa ttactccatg tatactgcct tctctcaatg
    24181 aatctacttt tattaattta ggaaaatttt cttctccaac actattgctt acatcttttt
    24241 ctataaactc catgtcactc ttggttaata tactacactg tataatcata gcttgtggat
    24301 ttaggacaca tacaagagtc cttataaagt ttgatattac agataaaatt tctttcttaa
    24361 atttattatc tttactttct aaataatctt ttcttaaact cataatataa tcttcaagat
    24421 ttaagttatt agagttatac ttaccaaatt ctatatttag ataacttaat tctccagcaa
    24481 aatttgtaga acctaaatat aactcaccat ttataactat acctgaacca ataccatctt
    24541 ctaaatacat aagggccata tttttatatt tgtctttata ctgtttttga taaatcccaa
    24601 tagtagtaat atttgtatca ttttctataa acactttagt attgtatcta ctctttatta
    24661 aattttctaa atttatacct tcccaacttt ttatagttgg tatattttta actacacctt
    24721 tttttacaac tcctggaaca ccaattccaa tagcccttat attgtttcct ttttcctttt
    24781 ttattaaagt atctataaca aaaaaagtat cttggttaac gttttcatat gtatccattc
    24841 ttatattaaa ttcttcttga taaattattt cacctaataa attggcaact acccctatac
    24901 aacaatttct ataataatat aaagctatta tgtgttctaa gtttgcattt atttcaaata
    24961 attgcgctct tcttcctcca gtagattcac ttagtccact tactttaacc tcatttttta
    25021 aagctaactg ctctacaatc ttattaactg tagctaaact caattcagta atgcttgaaa
    25081 tttctggttt tgttatagga ccctcatttt ttataatatt ttttattaaa tcttcattta
    25141 ctttttttat cgtttgcggt tttccaacta tatttttcat agacaacacc ttactttata
    25201 aactttattt attaagttta gtataatatt tacaagttaa atttgtcaat aaattttcaa
    25261 taaaacttta aaatcaaatt tctatattct ctgcttcata attattcttc ctgattaagt
    25321 tttattgttc atattccttt caactgttca tattagtttg atgatataaa tacatattat
    25381 agtattgact ataataaaat accctcatac aaagatagtt gcaattatct acctgaatat
    25441 atataataaa aatgtaaatt tgtcattgaa ctctatatta aaaacgaaat caatgttcag
    25501 atgaattttt caaaccagaa aggagtattg agtttatggg aattggaact tgtgcatagc
    25561 aaaggctatt gcacataata aaaaatctta tttttaattg tgagtggcag tagcctttgc
    25621 taatcctaaa gaataattat ttaggaggtg caaaatgagc tatcaaaatt caattgactt
    25681 actgcctaaa gaactgattg agcaagtgca agaatatata gatggtaaag tgatttatat
    25741 tcccaaaaag caagagcaca aaaatcattg gggtgaaaac acaaacacca aacaagtctt
    25801 agcttctcgt aattctcaaa tctgtataaa cttccaaaat ggaatgagta taaagcagtt
    25861 atcagagaaa tactttttga cagagaaaag catacaaaga atacttaaac agcaaaattt
    25921 ataattagaa atgtgtcacg ttttatgcgt ggcacatttt tattctaaga aagaacatga
    25981 ggtttttcat tcatacctat attgttataa ttaacttaac aaataaaaag gaggagaata
    26041 tatgtcatat tttcagtatc aaggtaattc ttgtttttat aaagaatatg gacaaggtaa
    26101 accgataatt ttcctgcatg gtaatactgg ttcttctaag atgtttaaag cacttatgcc
    26161 attatatgta gaaaacttta gatgtatctt aattgatttt ttaggtaatg gacagtcgga
    26221 tagagtttct caattttctc ctgacatgtg gcatgatgaa gccttacaga cgatagcact
    26281 gattgaacat ttgaattgtg gtaaagttgg cttaattgga acaagtggag gagcatgggc
    26341 agctgttaat actgggttag aacgtcccga tttagttgaa gcaattatag cagatagctt
    26401 tgatggacgt actcttaatg ataacttcat agataatcta atcactggaa gggaaaaatc
    26461 aaagcaagat attcaagcac gaaaattcta tgaatggtgt cagggaaaag attgggaaaa
    26521 tgttgttaat cttgatacaa aagctctatt gcaatgtgca aacgagaaac gacctttgtt
    26581 tcataaagaa ttgtgtaaat tagaaatgcc aattttattt acaggaagta aggaagatga
    26641 aatgtgtcgt cataatcttg aagaagagta caaacaaatg gcaactttta tttcaaaagc
    26701 aagtattcat atttttagtc agggaggaca ccctgctata ttgacaaatg cagacgaatt
    26761 tgcacagtta gcaaaagctt ttttcaagta ttaactaaaa atattaacta tttcataaac
    26821 attatttaga aaagaaatat taagttagca tttaaacaca caggtagtat tatcctatca
    26881 tatacagata taaaagaaac gccgatgacc cacaaaatat agtatttatt caggttctcg
    26941 gcgttttcct tatctcagtc taatactaaa tctcgactga caacatagaa tttttttact
    27001 actttttaaa cctaacctca gctgcctttg catgtgcata taatccctct tcttttgcca
    27061 gcttttcagc tacaggtgct aatgattgta tagctttttc attaaatatc tgctttgtac
    27121 aagttttgat aaaagttcca acccagacac caccagtata tcttgatgct ttaagtgttg
    27181 gtaacgtatg attggtacct gagacatagt caccaaatac ctcagcagaa agatttccaa
    27241 taaataaaga accatagtta gttaatctat cacaaatatc attatactca tttactgcaa
    27301 cttctaaatg ttctggtgca taaaaattac ttattttaat ggcttcttcc atatcatcaa
    27361 ccagtattat ctctccatta ttttcccatg aactatatgc tatgtccttt gttggcaaat
    27421 cttttaacat actttctata tttttttcaa cttcttgtgc tatttctaag ctatttgtca
    27481 ataatattcc tcttgcattt aaatcatgct cacattgtgc taataaatca gctgcaatat
    27541 aaactggatt tgaagtttca tcagctatta taagaacttc acttggtcca gcgacaaaat
    27601 caattcctac ttgtccataa cattgtcttt tagcttcagt tacaaactta tttcctggtc
    27661 ctacaattat gtcaactgga ttaatctttt ctgttccata tgtaaatgct gctatagctt
    27721 gaactcctcc agttgcatat atttcatcag cacctgctat atccatagct accaatgttt
    27781 taggatttat cttttcagta tttttcatag ttggtgaaca tgccactatt ctttttactc
    27841 ctgctacttt tgcaggtatt ataagcataa gagctgttga aaacaatgga tactgacctc
    27901 ctggaacata tgcaagacat gactctattg gaatattagt ttgacctaag attacacctg
    27961 gataaatttc tttttcaaaa gggttttcaa aacttagttt ttgagcttta gcaaattctc
    28021 ttatattttt ggcagcaatc tttaagttat ttataaattc gtcatctaca tgcttataag
    28081 cttcctttat ttcttcctta gtaattctaa attcatctct tgtatttcta tcaaacttct
    28141 cattatattt tttaagtgca acatccttat tatttcttat atcttctatt atagaactta
    28201 cttgctctct taatatcttt tcatctacct tttctttcat acttgatgtt ttaatactct
    28261 tcatttttta cctcctaatt ttacaatatc atactcattt attttacaat attatcctca
    28321 tttaagatat atcaataaat aaaaactccc accctctatc aaaagctaga ggatgggagt
    28381 ttaatcatat tgtactcacc tcactaactg agcactattt gtctaataaa tatacttata
    28441 tttcttctaa tcattatttt tttctgaatt ttctctttat tatacataat atgatatatt
    28501 ttaactattg atttgtcaat gaattttttt attttctaca gcagttacaa ttacatttat
    28561 ggcatttatg gcaacaatta ttattgtttc ctccattatt tccatttctt ctagcttcac
    28621 attccatacc ttcatcaaat aatctagcag cttctgctaa atattcaatt ccttgatagt
    28681 tgtattttga atattcatcc cagtattttt ctgcttcttc ccataatttt ctagcttcac
    28741 attcatatgc taatgcttct ttctttttct ctgaagattc acagaatgct tctcttgctt
    28801 ctctttctct tatctcagct tctcttctaa atctttcaca atttctaggt ctattttctc
    28861 ctgaatctga acaaccacag tttgaacctc ttctttctct atcttcatcg tatgctgctc
    28921 tttcatattc ttctctatca ttttggaacc atttagctgt agattctcct ctttcatacc
    28981 aatcttctga ataacatttt ttattttcca tataacatat ctctcccttt aattttaatt
    29041 tttttatgag atgttttgtt tcatctcata ctatatagta ttaaaatcgt caaaatatgt
    29101 tacatataaa cttatatttt ttctttttta acttattttc aacattttat tcattttttc
    29161 gcaatatcct ttatctttta ttagacataa actgttgttt atttcatatt cagaatcagc
    29221 ataaaccata atagcaagtt catcatcatc atttaaaata tcgaatttta ctaaacaatt
    29281 ctcaactata ttcagaattt catctttatt acatttttta aatctaagaa gtgctccaca
    29341 cttttctcta actattttat taacttgtat tttatcttta gtaaaagaat ctatacttga
    29401 aactctatta tctattatat ctataacatc tgaataaacc gcacttcctg ttgctaaact
    29461 tccagctcct ttacctacaa aagaaacttc tcctactgaa tctccattca ctattacagc
    29521 attgaactca ttgtctattc tagcaaggat attatctttt tctactaaaa caggtctaac
    29581 aaaagcactt actttatcat tttcatactt actttgacca atcaatttta ttttacaatt
    29641 taattttttt gcgtattcaa tatctttttc atctatatct gtaattcctt ctaaatacac
    29701 atctttccaa tatactctat tgtcataagc tagagttgat aatattgata gttttctagc
    29761 agcatcatac cctaatacat ctgactctgg atttgcttca gcaaaaccta attcttgagc
    29821 ttgtcttaat gccatatcat aagatagatt ctcatcatac attttagata atataaaatt
    29881 agtagttcca tttaaaattg catttatact atctatatta tttccttcta gtgattctat
    29941 tattggtttt aatactggta tacctcctgc aactgaagct tcaaatttaa tacttacttt
    30001 attttcactt gctaattttg caagctcatc tccacattca gctaacaaat ctttatttgc
    30061 tgtaacaaca tgaattttat tgtttaatgc tttttttata taagataatg atggttctaa
    30121 accacccatg acttcaacaa gtatatctat atctttatta aatatatcat ctatattatt
    30181 agttattata ttagaatatt ttttatcctt atgtttttcc aaattattaa ctaaaattcc
    30241 aactatttct atatttcttt tttctttatt attatctatt atatctataa gtccacttcc
    30301 tacaactcca tatcctagta ctccaatttt cactttattc tccattttat tttcctccta
    30361 aatttataat tttccgattt ttttttattt tcaatctttt attgcaataa aaatagactc
    30421 atccctatat aaagggacga gtctatactc gcggttccac ccaatttgat tttatcattt
    30481 attgataaaa tccactttac actttaacgc agttaacggt tagtcttact aaaacttttt
    30541 aaagtaactt cagaccacag ctcctaggta gttttcaatt tctcaaactt agaaagtctt
    30601 tcagcctgtg aactttcctc tctgatagtc gaattaaatt tactcttcct attcatcgct
    30661 tttatatttt ctataattat tatgattact gtttatattt ttaaataaca aaaaactctc
    30721 atccttataa cataaggacg agagtattat tcccgcggtt ccacctaatt tagttttcat
    30781 atatcatgaa aactcacttt acactttaac gcagctaacg actattctta ctaaaatcta
    30841 ctgatgcaga tgacttcaga aagtagctcc taggtagttt tcaatccctc taatttaaaa
    30901 agtcttccag ccaatggact cttctctctg gtaatcgaat tagatttact cttcctattc
    30961 attgcctttt tatttattgt gttgtaattt attttaaacc cttctaaatc aattgtcaat
    31021 acttttttaa aattttattt ttatacttaa acttataaat ccgaaaattc tatttctaat
    31081 tttttactta gttcattact gatttctctt ttatgattat atttttcaat attattttta
    31141 cttctaacag gtaatgaaac tataaattca ctcccaatac caagttgact ttttaaagat
    31201 atacttccct catgcaactc aaccaaagat ttcactaatg aaagtccaat gccacttcct
    31261 ttaactctgt aagaaagttc attgtcaatc tgttcaaacc tattaaatat tttatctatt
    31321 ttttcttttg gtatacctat accactatca gatattttca tattaaatat attatctttc
    31381 acatataaaa atatgtttat ttttccatct tccttattgt acttaattcc gttagaaagt
    31441 aaatttaata ttattctttc caatttttct gaatcaaata acattgaaaa ttcttctatt
    31501 tctgtatcaa atgtaagttg tatattattt tgacttgcga aatcagctac agacatacat
    31561 atttcctcta taaatgcaat tatatcagat tcttctggtt taaaatctaa atatcctgat
    31621 tgcatttttg taaagtctat aacattattt actaacctca gtattctatt tccattttgt
    31681 tttattatac ctaaatttct ttttaaagta tcatttgatt ctattaaatc tgcttctttt
    31741 ttttctatca cttgtaatga agatgatatt aaatttatag gtgttctaag ttcatgggat
    31801 aaatttgcaa aaaattccat cctagtttta tccatttcct cttctaatct ctttcttcta
    31861 cttatatccc ttgctgttcc aattattcca ataacacttc catagctatc aaaaaacggc
    31921 ctttttgtaa tctcaataca cttattttca tcatttatac tcacatttaa ctcaaatgta
    31981 actacttttt tattttcaat tacaccctta tcactttcaa atatcttatc tattaaaact
    32041 tcttcttctg atattttata actattcttc cctaataatt cttctttatt catacaagct
    32101 aaattcaaaa aacctgaatt aaatccaata taatttccat acacatcttt ataaaacata
    32161 ttctctggaa ttgagtgctc tatcatcttc attaaatctt ttgagttata gtttctatta
    32221 ctgtaatcac tatatattat attgtctatt atacctatag tacctaaaag ttcgtcttca
    32281 tcatacaaaa aatatttttc aaccataaaa aatgtttctt catattcaaa aagattttgt
    32341 agaaatcttt ttttactaga tactccttct ctataatcat ctctaaataa ttttcctgta
    32401 ttctctcccc atacttctat atcattttta ccaaagatat ctttcgttgt tttaatgttg
    32461 ttatttaata ttggtattaa attttcactg caatctatat atatgccttc ttcgtcttta
    32521 atataagcta ctttttcctt tttatcaaag gaatcgatta tctgtctata atcaaataca
    32581 attttaccaa aatacaaaat tatgtatgaa tcccctatta tctgtttttt tatatttatc
    32641 cagtttttaa gtgtcggaat gtaccttttt atacaatcca cttcatttga gaacaactct
    32701 ttgaaatatt ttttagactt aaatacaggc aatacatcta gtatattttt atcacaaata
    32761 ccttcttcta attgtaatac tttgcccata cccttactta tatattctac tcgatattct
    32821 atatcttctt gtctctttac aattctacaa tataaaaaag gtacaggtat attttttata
    32881 aaatttacat atttattatg ggtatccata ttctaaccac tacctttcaa tgttatttat
    32941 aataatactt cttaagtaat tatatgacaa ttttctatta attataacat attttataag
    33001 atatacttgt gaataaattt aattttatgt aatttattat ttgtttataa ttatttatgt
    33061 cgcttttttc taaatctaat tacagtagct tattctaata tattggtaat taggaacaca
    33121 ggcgcatata aaaacacatg actaaatact gcattatttg aaaatacaga cattaactca
    33181 ttaggactat aatttttatt tagtatattt tttgatgctt ctaccaattt tatctcttac
    33241 ttatcaatag tcatcttttc aaagatgatt ataaattaaa attatctcat aaaataaagt
    33301 ttgtcaagtt atactaaaaa taaaaaaagc ccagaaaaat tctagacttt ttaacttaaa
    33361 tgtcaatatt tctttattaa ttcaattaca tttataatat taaatcctat cttttatttt
    33421 aatactttac tttaacattt catgtcttat ctttctaaaa tacaatcttg aatttacctc
    33481 atagccaata acgatagacc aactactaaa atacatccag gtcataagta caattatttc
    33541 ggctatgctt ccgtagatta cttcatagtt agcatagtaa tttgtataat aagaatataa
    33601 aaacgatact aaaaaccaag caaaagtagc aaagatggca cctggcgcag attctttaat
    33661 agtcaacttt ttatttggag tatatttata taaaagtgta aatatcacta ttattgttat
    33721 tatacctaca gtgtatctta aaatatccca tatttttata aatatttcat ccaatcctat
    33781 aaggttaaaa ataaaataac ctattttttc tccataaact agaaatatca tagataaaaa
    33841 aataagtacc aaaagcatta ttgtaaataa aaatgatata gataaaattt ttaaaaaaga
    33901 cctagtttct ttaactttat aagctctatt cattccttta ataagagctt ttactgctct
    33961 tgaaaatgtc cacatagtaa gtacaaaact gactgctaaa aatttcaggt ttctattgtc
    34021 tattgcagaa actataattg aagataacac agcaaaagca ccttctggca tcatatttct
    34081 aaacaaagct atatacttat ttaaatgaag aattggtata taagctatag aacttattgt
    34141 aaatatcaaa aaaggaaata tagacaacat taagaagaaa gatacttctg ctgactttga
    34201 acttaattca ctgtaattgg attttgcaat tatatattca atcaatttct tatctatacc
    34261 aaacataaat atcccctttt ctatcaaatt aatttcatta tacaatatta attatactca
    34321 gattcattct ataatataat tatttaagta aaaataacac aaataaaagc tgaaaataat
    34381 aattctacta acatttccat attctactca atgaaagtaa attataattt tcagctaaat
    34441 aaatattatt acaaaattct cttataaaat aataatattt tattttaatt tttcgaccat
    34501 gtcatctata tattttttca acccttgaat ctttttatct gcttcaatat cactagctcc
    34561 ttttacagct atgtaaaatt taagttttgg ttctgttcca gatggtctta tagctaccca
    34621 agattcatct tccaaaaagt attttaaaac atcagattta ggaagattat ctataccatt
    34681 tttataatct tttttatcaa ctactttaat actatttata taatcaattt gattttctct
    34741 aaagtaagat attatttctt ttatcttttc tacaccttct atacctttta aagtcaaaga
    34801 tatagtctgt tctttgaaaa atccatactt tttataaagt tctattaaac catcatataa
    34861 actcatacct tttgaatagt aaaatgctgc catttcagat attaacaatg aagaaactac
    34921 accatcttta tctctagcat gagtcccaac taagtaacca taactttctt catatccaaa
    34981 tacataactt ctatttttat tttgttcaaa taattttatt ttttcaccaa taaatttaaa
    35041 gcctgtaaga acatttaata catcaacatt atttgattta gctatatctg ctccaaattc
    35101 tgacgtaact atggttttta ttattgtagg attttctttt aacttattat tttcttttaa
    35161 tccttctatt atataatgtg tcagcatacc accaatttga tttcctgtaa gtaaagcata
    35221 ctcacctgta gtagttttta ctgctacacc tactctatca caatctgggt cagttgctat
    35281 tacaaggtct gctccttttt cttttgcgag taatatacct ctatttaaag cttttttctc
    35341 ttctggattt ggataatcaa gccctgcaaa atttgggtct ggattttctt cttctggaac
    35401 tacaattata ttttcaaaac caacttcttt caatgctctt cttattggaa tattccctgt
    35461 tccacaaaga ggcgtataga ctatcttaaa gcttttacct acatttttaa ctaaatcttt
    35521 tcttataatt tggcttttta ctgcttctat aaatttagta tcttgtgatt catccaacaa
    35581 aaccaccaaa tttttattgg cccctgttaa ggttggtatt gtactataat catgtatact
    35641 atttactttc tctgttattg catttgcaat ctctggcata acttgtgctc catcttccca
    35701 gtatacctta tatccattat attctggagg attatggctt gctgttatga ctataccagc
    35761 aattgcattt aagcttctaa cagcaaaaga taattctgga gtagttctca aactatcaaa
    35821 tatatatgct tttataccac atgctgccaa agtatttgca gattctatac aaaattgtct
    35881 agacttgtgt ctattatcat gagctattac tactcctctg cttgtttctt cttttgttgt
    35941 gttttctaat atatagtttg ccaatccaaa agtagctctt ctaactgtgt acttatttat
    36001 tctattcgtt cctgcttcaa ttattcctct aagacctgca gtcccaaatt ctaaatcctt
    36061 ataaaatcta tcctctatct ctttttcatt attttttatc ttctccaact ctagctttgt
    36121 atcttcatca aaatatgaac cctttgtcca ctcttcataa gttttagtat aatccataat
    36181 aaacccctct ctactgtatg tattcattgc aactgacaca taaacaagca ttttaatgta
    36241 atctataact cttctatata cttattaatt gaattaataa aaatgtctgc cattattcta
    36301 tgaccttcct tggttggatg taacccatca aaatataatt ttttatatcc ttctagttcc
    36361 atatttttat tatattctga ataaaaatct acgaaattag tattaaatgt catacaaaat
    36421 ttttttatcc aataactata tgattctaag tttcgagata ctatattaaa atcagtaaaa
    36481 caactccaat ctgccctaac atttggaata tctggtttta atggtatacc tattataggt
    36541 atgatatttt ttgaatatga ctgatgcacc atagccatta tattagaatt aataatttca
    36601 tttctcgcac cagcaataaa atcattggtg ccacccatta taaaaactat atcaggagag
    36661 ttttttacaa catcttcatt aaacctagct aacataccac ttgttgtatc accatttata
    36721 cccttattta ctatttctaa tcgagtctct ttatttacaa tgtttgtcca tgaattactt
    36781 cgtgatactc caaatccata tgttaaactg tctcctaagc agactatttt catcttatcc
    36841 ccccttagat atatttttat gttttaagaa taatctaaca gtttagttaa ttcatatttt
    36901 atactttttt gtccttcaag taaagttatt ctattcacta atttaatgta gatatatatc
    36961 tatacttttc cttccccaat ttgaggcttt tccttcactg ttcataaaaa tatctattct
    37021 attaccttta attgcacctc cacaatcttc tgctacaaaa gtcataccca acttaggtat
    37081 atatactttt gttccatatg gtattacacg tgggtcaact gcaataacac cccatcttgg
    37141 agttgtacca gtagatgtta ttgtatctcc tgcatatgca gtggcaacca ctttcatatg
    37201 tttgcctcct cttatatttg ctcctcttga tgctattaat actttttctt tagttccata
    37261 ttttataatt tttgtagttg gctccttaga tattacttct tttgataaga cttttttgac
    37321 taactttcca tcatggtaag ttaaattata gactagtttt ttttctccct cttgaccttc
    37381 ttgataaact ttagattttc cttttaacaa attactatca tttacatgct ttgtatcaaa
    37441 tggaattttt tcaatctctt cttttttact tttagttacc ttaacaactt ttattttcat
    37501 atcatctttt agttctgtat ctaaacttgg attaatctta tcttcagaat tatactttac
    37561 accctgttca tccaaaagtt cctcaactgt tttcttaaag gaagatactt tttgctcttg
    37621 acctttaact gttaaactta tgtcttcttt atttaatata gaatatatac ttaccaaacc
    37681 cattaaaatt gatacactta ataaagatga tattattatc ctcttttctc ttttctccat
    37741 aataatctcc ttccatgttc aaattttaaa ttaatattat tcattacagt tttaacctta
    37801 tattatatat gtatattata gctttccaaa tttatgtaac ttttttgtaa cttctaaaat
    37861 tattaagatt aattttagac ctagaaaagt ccataaatat actgttttta gattatcaaa
    37921 gcttaatata atagttttaa gttatgtctt aagcaattat acattagtct atgagatata
    37981 aacataaata aaagaaagaa atgacactca acttaaaaca cattgtatgc aaataatgac
    38041 tatggtaaaa tgaactcata aataaattag aactttcgtg attagggaga gtaaagtgaa
    38101 atttgaacgg ttaagaacca gtgaaaataa attatattac aaggctatgg aattatataa
    38161 aattagtttt ccatttcatg aacaaagaaa atcttctttg caagcagaaa ttatgaaaaa
    38221 taaagaatat caattcaatt taatttatga taaaaaccag tttgtcggca taattttatg
    38281 ttgggaaaca gatagcttta tctatgtaga acatttctgc atttttccag aaatgcgtaa
    38341 taatagatat ggacaaagag cattagagct tttaaataag aaaggaaaaa ctgttatttt
    38401 agaaattgac cttccaattg atgagatttc aatttatcgc aaggcgttct atgaaagagt
    38461 aaaatataag acaaatgatt atgaacacat tcatccacca taccatgaga aatttaatgg
    38521 acataatctg attgtaatgt cttacccaga aaaattgtct aaaatggaat atgataattt
    38581 taatcagtat ttaagaaata atgtcatgag tttctaaatg taattcctat ttattgaact
    38641 gtgtaaaatt tcataattaa ataaaagaga tgtctcaaaa tagaaataag attctttttc
    38701 attgtttaaa ttataaagat atattcgatt tcatacaaag aaggatgcct catgaactaa
    38761 aaagttcatt ttgagacacc ctctttttca cacttaaagt taagaaaaca tgtgagattt
    38821 tatagacaaa attacatttt atataacaaa ctttatttta tttttgcaaa tttaggtttc
    38881 tatttttgtg taataagaca gataattcag atttttattt tgaaacctct atttttctaa
    38941 ataattgttt aagaccaaca aaaaaggtga tttaaatttt tattttgaaa cacttccttt
    39001 taatttgatt agtcttaagt cctatattaa atttttactt attcttcatt ttttaacatt
    39061 ttttccatct ctaaaatctt ttcaattaca cccttaaaaa ttggttcttc tctaagctca
    39121 ttataactat tatcattaag cataattaac aagtttgtat atatattaaa agtatataaa
    39181 tttttagact ctaccttatc aaaaaatctc atttgactta catccaaact ttcatgtata
    39241 ggatattttt ctaatatttc ttttagacta tataaggctt tttttgcttc ttctttcatg
    39301 ttaaaaaata aaaaaattag agacaattcc atataatcca tatacatact aaaaaatgca
    39361 cctttatctt cactgagcat tttttttatt tctaaagaaa gatttgcata ccccagcaac
    39421 ttatttttta tatactccat ttttctctca attgcatttt tattagaatt tctattttcc
    39481 aattttttct ttaattcctt gtattgattt ttgtatacat tgattattcc cttacaatcc
    39541 atgctcatat tgaatacatt actaaacaat ttattttgaa gcaatatttc tgcttcttca
    39601 agtctatttt gctttaaata tatcatagct aaattaacac ttgtatcccc tattgactta
    39661 tgtatcatat ttaagtacaa ctctgccttt tcataatcct ccaacataca atatgcgttt
    39721 gataatataa taagagctgt ttcttttatt tggatatcat ctgtatttct tactatatct
    39781 tcaagaatat tcgttgtttc ttttactctg ttattaagtt cactcttacc aaatttatat
    39841 gaatacatct gatacagcga acatatcctt agcttcaaca aatagctttt aggatacttc
    39901 tctatataca tattagaaag tttttttaca gattctaagt cttgtctaca ctcttctttt
    39961 ggttcttctg ttgataaatc aatttcaatt ctagcaaaac ctgattctaa ttctttataa
    40021 attttcatta cttcttcttc acttaaatca atcttgtaat ttaaaagctt atcaatagac
    40081 acattaaaaa agtctgctaa taaaggtaat aattctatat ctgggtaaga atttccggta
    40141 tcacatagtt aaagatattg gtatcattca atcaattttg ccgataaagc ggtagaagat
    40201 ttctacctcc tgttcccggc ttccgtcctc acctttgacc gcttcatgga caacgatttt
    40261 ttcaatcagc atattcaaaa gttcggcggt cagttctgtt ggattgacgc actctttcat
    40321 caaggcaatc catttttctg cgtctacggc gttctggctt tcagtggcga tagcggattg
    40381 aagctgttca atcttttcgt ccagttcagc ctgttcgctt tggtacttcc cggacagcat
    40441 agagaagttg tattccgtga tacgccccgc cgcccagtcc tcatacatcc gggcaaacaa
    40501 agtatcgact tcggcttttc gcttctctgc ctttttcagt tctgcggcct gcttctttct
    40561 tgcggcagcc tgtcccttat cagtggcatt taacagctgc ttcaagagcc gttcttcatc
    40621 atgctgtacc agccccgacc agtattgcag acgggagagg acataggcat aaagcacatc
    40681 ataacggata tagtgcatgg agcattggcg tgtgccctgc ccgtacttgc tacaatgata
    40741 atggccgtaa ggcttgctgt tctgcctgtt ctccccatag gacagcgacc agccgcaatc
    40801 cgcacatttt accaatccgg aaaaaatctg cgttgtacca tccttgcact tcctgcggcg
    40861 gtttgctatc tgctcctgta cctgccggaa aacctgctcg ctgataatcg gctcctgcgt
    40921 gttctccacc cgcacccatt cactttgggg cttgcgtacc ctccgcttgt tcttaaagga
    40981 aatgttcgtt tcccggtaat gaatggtatg gccgatatag gtttcgtctt tcataatgct
    41041 cttgacctgg gctatcgtcc atgcgtagct tttttcctcc ggcgcacccg catagatgtt
    41101 tgcaaaagtc ccgtcacgct ggaagttgat aaatcccggt gtgggaacct tttccgcaat
    41161 cagtattctt gtgatactgg ccgcccctct gccatgaata gcaaggtcaa aaatcttctc
    41221 cactatccag cgggtttcct cgtcgattat cagcttgttt ttgatttccg ggtgtttcct
    41281 gtacccgatg ggagcatagg cgccgatacg ctgtcctgtg gcaaactttg ctttgaaagc
    41341 ggcctttacc ttgcggcttg tatctttcgc aaaccattca ttgaacaggt ttttgaacgg
    41401 gacaaaatcg gaaagccctt tctctgtgtc ctcattctcc gcaacggcga tgtagcgaac
    41461 ccgtttctcc gggaacagaa attccagata gtagtccatc atcacatgtt cccgcccgaa
    41521 acgggacagg tctttcgtga caatgcagtt tacttttccg gcttccatat cgtccatcat
    41581 gcgctgaaaa tccggccgtt caaagttcgt ccccgaccag ccatcgtcca catactcacc
    41641 gactacatga agcccctgtt cctttgcgta ctgttgcagg attgtccgct gtgtttcaat
    41701 gcttacgctg tcaccatagt tttcatcgtc ccggctcaat ctcatataaa gcgccgtgtt
    41761 gtaaatcgta gtattgtaag gttgtttcac cgttaaaaat cctccttcta aagaaacaac
    41821 ccacgcttac aatacttttg ctctatggca attatatcat aagcgtgggc gtgttatcaa
    41881 tgatgggcta tcaggttgaa gcggcttttt ctgcggtatg ccgcaccaca tctacaatca
    41941 gatcaccgag gggcttcccg cctgcggcga agtgttcgga gatttttata cggttgttcc
    42001 cacatacaaa atactgcgtg ccgttctctg cttgtatgac ctgccctgtc cggggtgtga
    42061 aaatatcgct ttcgcttttt gccatccagt gtcctccata ttcagttttc aaggtacaat
    42121 gccgcacaaa ggcggcgaaa atttctgctt atatctatca cctttccttt accgtgtgcc
    42181 gctgctgccg tttcagttcc gccagtatat ccggcgggat acggtcaacc agccgctgta
    42241 aattgtccag ttcgcttttc agctttgccc gttccatcgt atctttcatc tttccttttt
    42301 cgctggcctt tgcccttgct tccagcttct cattctccgc caacaggtca ttgattgtga
    42361 ccttgtactt tttcagttgc ccggagaagt tctccatctg cgggaaccac tttttcagca
    42421 tggagagggc ttcctctttt ttctttccgg cgttcagcgg gttaatgccg tcaagggcgg
    42481 cttcaatggc tcttgcctgc cgggagaggg aaaccgcctg tttgaaaatc cgggtgggga
    42541 tatgcttccg gcctgtcttg ctggcacttt ccccacgctc caagtcggga tatttctcca
    42601 ccatataggc gtgaaaatcg tcctgccact tcgtcaggtt tgcccggttg ccgataatct
    42661 ccttagcgca caggcggttg tcctttgtca gcggaacaaa ggtcaaatgc aggtggggcg
    42721 ttttctcgtc catgtgtacc accgccgaca cgatattttc ccgtcctacc cggccaatga
    42781 ggaaatctgc cgccctttgg aagaacgcct gtatctcttt tggggatttc cccttgaaga
    42841 actccgggct ggcggttatc agcgtatcga caaaccgtgt gctgtctttc ctcgtccggc
    42901 atccagcctg ctcgatacgg ctctgaatga agtggtaata gcgtccctcc ggcttgacga
    42961 tatggaagtt gtatttactc cggcttgtgt caatgtcggg gttgctggcg tattgttctt
    43021 tctgtctttc gtgatgggct tccagcggcc ttgccgggtt gcccttgtgc ttctcaaacc
    43081 gcaaaattgc gtgttgtgcc attctgctcc tttccccatt cctttcctat ccggggtttt
    43141 ccacagggaa aatcccgccg gaattatctc tgatacgata ggaatggaat ggatataaat
    43201 aaatcatttt aatctttgta tttctatata ccgtccggtt ttcgtacttc aagaggggcg
    43261 gtttccgtac ttcatgggta cggttttcag tcctccggcg taccagaacg ggatttcttg
    43321 aagtcagtgt ttggaaccgc ttcataggat tttgggaaaa tgcggttggg ttttccacag
    43381 ccctgcttct ggatttccac cagtccggcg tattgcagtt cccgcagggt gttcaccgct
    43441 ttctgccgcc cacagtggag caggtcaacc acctcgcaga tggggtaata caggaaaatc
    43501 cgtccgcagt catccgccca cccattcttg cgggacaact ctgtccggcg caggataaag
    43561 gcgtacagaa cctttgcctc gttggacagg ggcttgaatg tgggggcttc aaagaggaaa
    43621 ttcgggagcc gggtgaagct gaacgccttt tccggctgat ggatatagat ggtatttgtc
    43681 atagtgcgtt ttgtggacgg ttagaagccc gttttccggg gcgggcgata ctttatacca
    43741 cctgccccgg tttggggctt gcaaagcctg ataaatcaag gcttttttcg ctcctaactg
    43801 tccacagcag acctcctttt ccgttcactt tctgttttct gccgcctgtg aactctggcg
    43861 gcacagccgg ggcagtattt tgcccggttg gatttgggga cgaacacacc gccgcagacc
    43921 acacagcgtt tcaagtcctt atcccggaaa atctccgctt ccagcgtccc gtccagcggc
    43981 aagaccgccc agcggaacca cttacagcag accgagaaag aaaccgtctg cgggcaggtg
    44041 caggtgtccc catcgtcaag gacaatgcag ttgccgtcct cacagcaaca gcactcccgg
    44101 cgtatcaggc tggccgcctg tttcctctgt gccggtgtca tgcggtaaag ggaaccgtcc
    44161 ggcctgcgtt ccagcggcgg caagtcttta taggggttat ttctcatgcg ttccctccat
    44221 tttgctttcc gttgccgttt tccccgaaag gatttccagc tttggcacgc tccccgcagc
    44281 ttcgggacat tttgtcccga agtccggttc cttgcggtgc ttccccagcc ggggtattcc
    44341 ttttcggacg gtcattctgt tttcaaggtg ctgtccatcg atgaactatc ctaagtctac
    44401 acctaaataa ccgcccgtcc cgtatatcca agaggtagga aatccgcccg gaaaactccc
    44461 tatttccacg ctctcggaat tgtggtataa tggaagaaaa tatgagggag gagaattttt
    44521 gaacataatt agactgtcaa aggagcacat tgaggaatgt gcagatttat ttattgatgt
    44581 gttcacaaaa gccccctgga atgatacata caattccaaa gggcaggtga taagttattt
    44641 tcaaaactac ataaacaata actactttgt agggtatggc ttaaaagacc aaaactgtat
    44701 gattgctatc agcgtgggaa tgaagaaacc atggattaac ggaatggaat attatatcga
    44761 ccagttttgt gtaaagactg acttgcaagg gaaagggatt ggaagttatt ttttgaaatt
    44821 gattgaaaat gagatacaga cagaaaaaat ggaagaagtt ttaattgatg ataatatggt
    44881 ttttgatatt gataatttaa aaggatttct taatgatacc agttcatttg ggtttatagc
    44941 taaagaaaat aataaaatta taggatttgc atattgctat acacttttaa gacctgatgg
    45001 aaaaacaatg ttttatttac actcaatagg aatgttacct aactatcaag acaaaggtta
    45061 tggttcaaaa ttattatctt ttattaagga atattctaaa gagattggtt gttctgaaat
    45121 gtttttaata actgataaag gtaatcctag agcttgccat gtatatgaaa aattaggtgg
    45181 taaaaatgat tataaagatg aaatagtata tgtatatgat tatgaaaaag gtgataaata
    45241 aatgaatata gttgaaaatg aaatatgtat aagaacttta atagatgatg attttccttt
    45301 gatgttaaaa tggttaactg atgaaagagt attagaattt tatggtggta gagataaaaa
    45361 atatacatta gaatcattaa aaaaacatta tacagagcct tgggaagatg aagtttttag
    45421 agtaattatt gaatataaca atgttcctat tggatatgga caaatatata aaatgtatga
    45481 tgagttatat actgattatc attatccaaa aactgatgag atagtctatg gtatggatca
    45541 atttatagga gagccaaatt attggagtaa aggaattggt acaagatata ttaaattgat
    45601 ttttgaattt ttgaaaaaag aaagaaatgc taatgcagtt attttagacc ctcataaaaa
    45661 taatccaaga gcaataaggg cataccaaaa atctggtttt agaattattg aagatttgcc
    45721 agaacatgaa ttacacgagg gcaaaaaaga agattgttat ttaatggaat atagatatga
    45781 tgataatgcc acaaatgtta aggcaatgaa atatttaatt gagcattact ttgataattt
    45841 caaagtagat agtattgaaa taatcggtag tggttatgat agtgtggcat atttagttaa
    45901 taatgaatac atttttaaaa caaaatttag tactaataag aaaaaaggtt atgcaaaaga
    45961 aaaagcaata tataattttt taaatacaaa tttagaaact aatgtaaaaa ttcctaatat
    46021 tgaatattcg tatattagtg atgaattatc tatactaggt tataaagaaa ttaaaggaac
    46081 ttttttaaca ccagaaattt attctactat gtcagaagaa gaacaaaatt tgttaaaacg
    46141 agatattgcc agttttttaa gacaaatgca cggtttagat tatacagata ttagtgaatg
    46201 tactattgat aataaacaaa atgtattaga agagtatata ttgttgcgtg aaactattta
    46261 taatgattta actgatatag aaaaagatta tatagaaagt tttatggaaa gactaaatgc
    46321 aacaacagtt tttgagggta aaaagtgttt atgccataat gattttagtt gtaatcatct
    46381 attgttagat ggcaataata gattaactgg aataattgat tttggagatt ctggaattat
    46441 agatgaatat tgtgatttta tatacttact tgaagatagt gaagaagaaa taggaacaaa
    46501 ttttggagaa gatatattaa gaatgtatgg aaatatagat attgagaaag caaaagaata
    46561 tcaagatata gttgaagaat attatcctat tgaaactatt gtttatggaa ttaaaaatat
    46621 taaacaggaa tttatcgaaa atggtagaaa agaaatttat aaaaggactt ataaagattg
    46681 attatataat atatgaaaag ctattataaa agacattagt attaaatagt ttaaaaaaat
    46741 gaaaaataat aaaggaaaaa tgaacgcaat cattttgaat actgaacgag gttttccggc
    46801 cgaaaaattt tataagaaaa acggcttcca atcagttgat gaacttatta ctctcacaaa
    46861 ataaatatcg gccgcttaag cggatgagga ggagagcatg gaactatcca tacaagaacg
    46921 attgaaagac ctgcgtgtag agcgtgggct gacgctggaa cagcttgcgg agcagaccca
    46981 cctctccaag tctgctttag gcagttatga gggggacaat ctcaaggaca tcagccatta
    47041 tgcccttatt gagttggcaa agttttacga agtgaccgtt gattatctgc tgggccgttc
    47101 tcaaacaaga aatcacccaa acgccgatct tgcagacctg catttgagtg atgacatgat
    47161 tgaactatta aaaagcgggc tggtggacaa ttctcttttg tgtgaactgg cgacccaccc
    47221 ggattttccc cggctcatgg ccgaccttga aatctatgtg aacggcgtgg caggaaaaca
    47281 ggtgcagagc gcaaacgcca ttgtggacgc tgtgagtgca acaattatga aacaacacaa
    47341 tcccggcttg actgacccgc agctgcgaca gcttatcgcc gcccatattg acgacgacag
    47401 cttttgccgc tatgtgatac agcaggacat aaacaagata gccctcgacc tgcgggaagc
    47461 ccataaggac gattttttca gtgtcccaga ggacaaccca ctggaagatt tcttgcagac
    47521 cgcagaggaa accaccagag aggacagcga cccggagcag gcggctatga tgtttatctg
    47581 caagcggctc aaactgaatt acaagaagct gttcgaggaa gaaaagaaat ggctaaaaag
    47641 aattgcacaa aagtctgact tgctgaaaaa tccaaagccc cagcggggaa gaaagtagga
    47701 gaacggaaaa tcgagatttg gagtggtgaa ctatgaaact atttgattgg ggtgggagag
    47761 aataaagaca tacttctttg atgaaccaga ctataaaatt taaggagagc aaggaagtat
    47821 gaacacaata aaattaagac caatcattac cgttgaagaa aaaaacacat ttgtaagtga
    47881 aatacaagag gcttttcaaa tttcttatgt gtcagagttt ggagaatttg aaaagcaagt
    47941 tcttcccacg gaagatatag aagaatcttt taacgcaaaa ggcgctgaag catatatcgc
    48001 agaaattggc ggagaaagaa tcggcggagc agtcattgta atagatgata agaccggcta
    48061 caactcactt catcttttat atgtgaaatc aagcgcacaa aatgctggaa atggatttaa
    48121 gatatggaaa gcgattgaag aactacatcc tgaaacaaga atttgggaga cgcatacccc
    48181 atattacgat aagcggaaca ttcattttta tgtaaatcgt tgtggtttca agattgtaga
    48241 attttttaat cctaagcaca aagacccaca ccaaataagt gaaagcgcag gcaacatacc
    48301 aaaagagaac aattatttct ttagatttga aaaagaaatg aaatccagat agtaatttat
    48361 cggaggatag caaagccagc cgagccagtc aacggtcaag atgaac
//
LOCUS       DAEPXF010000422        30142 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain HB27
            SAMN13376389-rid12479423.guided.029, whole genome shotgun sequence.
ACCESSION   DAEPXF010000422 DAEPXF010000000
VERSION     DAEPXF010000422.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13376389
            Sequence Read Archive: SRR10518432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 30142)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30142)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001069624.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001069624.1
            Genome Coverage       :: 315x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/15/2021 01:54:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,165
            CDSs (total)                      :: 4,076
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 89
            rRNAs                             :: 10, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 10 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 19 of 69
            Pseudo Genes (incomplete)         :: 44 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 10 of 69
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30142
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13376389-rid12479423.guided.029"
                     /strain="HB27"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="China: Hebei"
                     /collection_date="2017"
     gene            complement(414..1607)
                     /locus_tag="KRL67_004053"
     CDS             complement(414..1607)
                     /locus_tag="KRL67_004053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005336078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HBH1524822.1"
                     /translation="MKEKRRDSKGRILHTGESQRTDGKYLYKYVDAFGNTKYVYAWRL
                     TPTDPTPKGKREKPSLRELEQQIRRDIEDGIDSTGKKMTLCQLYAKQNTQRANVKKST
                     QKQREQLMRLLKEDKLGARSIDTIKPSDAKEWALRMKDKGFSYNTINNHKRSLKASFY
                     IAIQDDCVRKNPFDFKLSEVIENDTKEKVALTEEQEQALLSFIKADNVYHKYYDDVLI
                     LLKTGLRISELCGLTVADIDFKNEVVIIDHQLLKSKEQGYYIETPKTKSGARQVPLSR
                     ETIQAFQRVMKKRPKAEPFVIDGRGNFLFVNHKGKPKVAIDYNMLFVRMVEKYNKHHK
                     DNPLPHITPHTLRHTFCTRLASKNMNPKDLQYIMGHSNISITMNWYAHASIDTAKSEV
                     QRLIA"
     gene            complement(1686..1889)
                     /locus_tag="KRL67_004054"
     CDS             complement(1686..1889)
                     /locus_tag="KRL67_004054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002262306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HBH1524823.1"
                     /translation="MNNNDIPVWEKYTLTIEEASKYFRIGENKLRRLAEENKDAGWLI
                     MNGNRIQIKRRQFEKVIDKLDAI"
     gene            complement(1889..2980)
                     /locus_tag="KRL67_004055"
     CDS             complement(1889..2980)
                     /locus_tag="KRL67_004055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018594174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HBH1524824.1"
                     /translation="MTTHKNLSVKIDYISIVFETATAEDVIMHILDLPTDIFNVYPAT
                     IKFKTYQARWQIGDIYVSGDARKTEDNPQGLGCYLVMTGRGCDDIFRILDSRNYTFGD
                     MFRRCERRYGLDNFHFTRLDIAIDDKNEKPFFTIEQIKKKCEKEEFISNSEGYHFDES
                     KFDDFDTAKTVYIGAGKSGLSYRFYDKDKEVCSKHNKTLDEVGSWKRTEMQLRDDKAH
                     AFAMTFKDRPLELGELAFGLLANNLRFVVPNRNESNKSRWKTCRFWERFLGAMEVLKL
                     QVPKLHNSLEETQQWLTEGGVISAVKSFYFLEEHDALGGLEKVGTMLDKARYSNSLSS
                     KLTAHLQRIDRTDLIPYIQYNTKHGKGGI"
     gene            3452..4087
                     /locus_tag="KRL67_004056"
     CDS             3452..4087
                     /locus_tag="KRL67_004056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBH1524825.1"
                     /translation="MEHYEKKISFLGENIQTIRKHRGMKQQELADKIGINMQSLSKIE
                     RGVNFPTFDTLEKIMDVLGVMPNELLSGEWKYVNQSEKEVCQFLRIEERLNAELKHGH
                     YDNFFDSEEEWLEYELEKLREYITDYINGKSIVASDLYPIKEFIQHLKFQKLLDRYDD
                     LYSMDMFGESIEGHKYRTPYQVVKMINPNSKEDMELLREVLKNNHFDDEDE"
     gene            4438..5049
                     /locus_tag="KRL67_004057"
     CDS             4438..5049
                     /locus_tag="KRL67_004057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008728833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HBH1524826.1"
                     /translation="MNITKQKSQKTFDKQAADYDTNIQGEHARKLYKPIIENLKNKNI
                     HSILDLGCGTGALLKEIKELNIAEQLFGIDISPNMLEIAKNKLGNDATLILGDSERLP
                     FEDSSFDAIVCNDSFHHYPQPDIVEKEVSRCLKQNGVFIIGDCWQPIGARQIMNYYMK
                     HSNSGDVKIYSKKEMLLLLSKDFHEIEWKSFGTRSCLIIAYNN"
     gene            5073..5633
                     /locus_tag="KRL67_004058"
     CDS             5073..5633
                     /locus_tag="KRL67_004058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008728836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBH1524827.1"
                     /translation="MRISKEPEERKQEILETAIKLFSVNGFEKTSISDIAKEIGIAQG
                     LCYRYFPSKDVLFQSAINEYSNILVANMTKNINIKKDSLKDILRKMILFAEQEDDTYY
                     PVFHNNQNKNFHNLLTLNVCEKLFPIVSEIIERANYTNEIQVNDVEMYASFCIYGQLG
                     IILNTDISIKEKSSRIKAFFRDLFRL"
     gene            6261..7310
                     /gene="cfr(B)"
                     /locus_tag="KRL67_004059"
     CDS             6261..7310
                     /gene="cfr(B)"
                     /locus_tag="KRL67_004059"
                     /EC_number="2.1.1.224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(8))-methyltransferase
                     Cfr(B)"
                     /protein_id="HBH1524828.1"
                     /translation="MQQKNKYIRIQEFLKQNKFPNYRMKQITNAIFPGRINNFNEITV
                     LPKSLRDMLIEEFGESILNIVPLKAQQSTQVSKVLFGISGDEKIETVNMKYKAGWESF
                     CISSQCGCNFGCKFCATGDIGLKRNLTSDEITDQILYFHLQGHSIDSISFMGMGEALA
                     NVQVFDALNVLTDPALFALSPRRLSISTIGIIPNIKKLTQNYPQVNLTFSLHSPFNEQ
                     RSELMPINERYPLSDVMDTLDEHIRVTSKKVYIAYIMLHGVNDSIEHAKEVVNLLRGR
                     YRSGNLYHVNIIRYNPTISSRMRFEEANEKCLVNFYKELKSAGIKVTIRSQFGIDIDA
                     ACGQLYGNYQKTNSQ"
     gene            8011..8952
                     /locus_tag="KRL67_004060"
                     /pseudo
     CDS             8011..8952
                     /locus_tag="KRL67_004060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008728838.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
     gene            9144..9563
                     /locus_tag="KRL67_004061"
     CDS             9144..9563
                     /locus_tag="KRL67_004061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008728841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HBH1524829.1"
                     /translation="MNPSSFEHIVRIQFNALMMTVIKCTVKNRNRQFARRSKREVLFC
                     ELSDTKNVECVTKDNYSCDYISFEVLNFTIQISNEKLAVALYKLSNKERDVILLRYFQ
                     SMSDQEIAELYHVSRSAIYRRRSNGLKKLKTVLKERN"
     gene            9568..9807
                     /locus_tag="KRL67_004062"
     CDS             9568..9807
                     /locus_tag="KRL67_004062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004609551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBH1524830.1"
                     /translation="MKKEYGYPTLKVICQASAGNETAIREILKFYDAYICKLCLRPFY
                     HSESGKIIMQVDEELKGQIHTEMMKAILKFEIRVK"
     gene            9940..10339
                     /locus_tag="KRL67_004063"
                     /pseudo
     CDS             9940..10339
                     /locus_tag="KRL67_004063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434143.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
     gene            10327..11265
                     /locus_tag="KRL67_004064"
     CDS             10327..11265
                     /locus_tag="KRL67_004064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HBH1524831.1"
                     /translation="MENLILSINVVLPLFLTMSLGYVLKKLGLFDTHTLNKMNNVCFK
                     SFLPLLLFFNIYNTDLDGSLNFKLMAVAVIIIFSIFLILVFIIPKIEKDNKTRGALIQ
                     GVFRSNFVIFGIPVTISLFGDKSIGVTSLLIAVIVPMFNVLSVVILEIFRGGKVSIKH
                     MIKSVLTNPLIIASFIGLTMLILNINIPTFLQKTIGDISKIATPLSLIILGGSFSFSA
                     IKGHIKPIILGVCGKLILVPAIFLPISIFLGFRNVELASLMIMLSAPTAVSSFTMAQQ
                     MDSDSELAGQIVVFTSAFSIITVFLIIFILKQNNLI"
     gene            complement(11631..11855)
                     /gene="secG"
                     /locus_tag="KRL67_004065"
     CDS             complement(11631..11855)
                     /gene="secG"
                     /locus_tag="KRL67_004065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HBH1524832.1"
                     /translation="MSNILMGVQVVLGILLVISIMPQDSKNALPSEFGGEGSQAYFKP
                     KGKQAFLARVTKITAVLFFINALALLIVKK"
     gene            complement(11969..12478)
                     /locus_tag="KRL67_004066"
     CDS             complement(11969..12478)
                     /locus_tag="KRL67_004066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBH1524833.1"
                     /translation="MNLEIIRATGENATEMGFIHSYSWKKAYKGIIPDNIIDEFTSEK
                     RAEIFHNVIPKAEEEYYLFKVDGIPAGFASLNKSHEENAPKYIGEIYSIYFHPDFWGT
                     PVTKKGLQFCIDRLRNLGYSYITIWVLKDNTRAIKFYKKNGFTCDDFEKEIYIGKKLL
                     EIRYSKKIQ"
     gene            12558..12989
                     /locus_tag="KRL67_004067"
     CDS             12558..12989
                     /locus_tag="KRL67_004067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HBH1524834.1"
                     /translation="MSMNAIKELSNLWHIVGMQMKTEMCSGKYPTIYGISLVELSILE
                     IVEQHPNSMMKEISSTLELPKSTLTSAVKRLELKKLIKRNPCIKDKRAYNLDLTELGL
                     KAQQEHREIEATVFKNILHQLDKDEIETFIHIFSKAVNKIR"
     gene            complement(13089..14381)
                     /gene="eno"
                     /locus_tag="KRL67_004068"
     CDS             complement(13089..14381)
                     /gene="eno"
                     /locus_tag="KRL67_004068"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HBH1524835.1"
                     /translation="MSVIELVYAREVLDSRGNPTVEVEVVLEDGAMGRAIVPSGASTG
                     AFEAVELRDGDKGRYLGKGVETAVANVNEIIAPEIEGMDAFDQPAIDAIMIELDGTPN
                     KGKLGANAILGVSMAVARAAADEIGLPLFQYLGGVNAKQLPVPMMNILNGGEHADNNV
                     DVQEFMILPVGACCFKEGLRMGAEVFHSLKKVLGEKGLACGVGDEGGFAPNLGSNREA
                     LELIVEAITKAGYKPGEDVMLGLDVAATEMYNKETKKYVLAGEGKELTAAEMVALYED
                     WSNNFPIITIEDGLDEEDWDGWKLLTEKLGNKLQLVGDDLFVTNTERLEKGIENGVAN
                     SILVKVNQIGTITETLDAIEMAKRAGYTAVISHRSGETEDSTIADLAVAVNAGQIKTG
                     APSRTDRVAKYNQLLRIEEMVGEQARYCGLKSFYNLKK"
     gene            complement(14969..16501)
                     /locus_tag="KRL67_004069"
     CDS             complement(14969..16501)
                     /locus_tag="KRL67_004069"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003228330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HBH1524836.1"
                     /translation="MMKKPVALIIMDGFGYNKDVKGNAIAESKTPNLDRIKKEYPNTL
                     INASGLDVGLPDGQMGNSEVGHTNIGAGRIVYQDLTRITKSIKDGDFFTNKVLCEAMD
                     NAKENSLHVMGLLSDGGVHSHIDHLKAIIKMSKDKGVQKVYVHAFTDGRDTDPQSALE
                     YAKEVQASMDEIGVGEFATVSGRYYAMDRDKRWERVELAYNAMVRGIGEKANSIEEAI
                     QNSYDDGKNDEFIMPTVIMKDDKPVGSIKENDSIIFFNFRPDRARQITRALVCEEFDG
                     FKREDIKSFFVCLTEYDITIENVHIAFGPQSLANTLGEYLAKNGKTQLRAAETEKYAH
                     VTFFFNGGVEEPNKGEERLLIPSPKVATYDLKPEMSAYELTDKALEKLGEDKFDFIVL
                     NFANPDMVGHTGSIEAAVKAVETVDTCVGKLVDKIVELGGSAIITADHGNAEYMLDPE
                     TGKTVTAHSINPVPFIVVGQEYESAKLLDGGRLSDIAPTVLDMMKLEKPEEMTGHSLI
                     SK"
     gene            complement(16510..17253)
                     /locus_tag="KRL67_004070"
     CDS             complement(16510..17253)
                     /locus_tag="KRL67_004070"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HBH1524837.1"
                     /translation="MRKPIIAGNWKMHKTIKEALEFVNEVKYKVNSDKVEAVICAPFT
                     LLKDLKEATKGTNIKIGAQNMHFEEKGAFTGEVSPLMLKEIDMDYVVIGHSERRQYFN
                     ETDETVNKKVLKALEVGIDPILCVGETLEQREAGKTKDVCKIQVEKALENVLKDDLAK
                     VVVAYEPIWAIGTGKTATAEDANDVISYIREVIKGLYGELANEVRIQYGGSVKPSNVA
                     EIMGQSDIDGALVGGASLASNDYLDLVNF"
     gene            complement(17279..18481)
                     /locus_tag="KRL67_004071"
     CDS             complement(17279..18481)
                     /locus_tag="KRL67_004071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HBH1524838.1"
                     /translation="MSMLNKKTIEDIDVCGKKVLVRCDFNVPLQDGVITDENRLNGAL
                     PTIQYLISKGAKVILCSHLGKPKGEAKPELSLAPVAKRLSEMLGKEVVFAADDNVVGE
                     NAKKATEKMENGDVVLLENTRYRKEETKNEENFSKELASLAEIFVNDAFGTAHRAHCS
                     TVGAGEFLQERVCGYLIQKELKFLGEAVANPVRPFTAILGGAKVSDKLAVINELLEKV
                     DNLIIGGGMAYTFLKAQGYEVGTSLLEIDKVEYAKEMMEKAKNKGVNLLLPVDVVMAD
                     HFAPDATPIVTEDANVKEDYMGLDMGPKTIANFVKTIKESKTVVWNGPMGVFEFENFA
                     NGTLSVARAMAELTDATTVIGGGDSAAAVNQLGFGDKMTHVSTGGGASLEFLEGKELP
                     GIAALDNK"
     gene            complement(18703..19710)
                     /gene="gap"
                     /locus_tag="KRL67_004072"
     CDS             complement(18703..19710)
                     /gene="gap"
                     /locus_tag="KRL67_004072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HBH1524839.1"
                     /translation="MVKVAINGFGRIGRLALRKMMEQQDKFEVVAINDLTDAKMLAHL
                     FKYDTAQGRFNGEIEVKEGAFVVNGKEIKVTAERNPADLPWAELGVDIVLECTGFFTS
                     KDKAEAHIQAGAKKVVISAPATGDLKTIVFNTNSDILDGSETVISGASCTTNCLAPMA
                     KVLNDKYGIEKGLMTTIHAYTNDQNTLDGPHPKGDLRRARAAAGNIVPNTTGAAKAIG
                     LVIPSLKGKLDGAAQRVPVVTGSITELVCTLGKNVTVEEINAAMKEASNESFGYTEEM
                     LVSSDIIGISYGSLFDATQTKVMEVDGKQLVKVVSWYDNEMSYTSQLIRTLGYFAQLA
                     K"
     gene            complement(19770..20792)
                     /locus_tag="KRL67_004073"
     CDS             complement(19770..20792)
                     /locus_tag="KRL67_004073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HBH1524840.1"
                     /translation="MKNLLEIQKKLIPQAIELMERRYSILRQISLSEPIGRRTLSNVL
                     EISERIIRSETEFLKEQGLIDVAVSGMTITKEGSDLLDKLKDIMSDIMGLSKLQDRVR
                     EKLGIKRVILVPGSCDENGSLLKDVTRYGCEYFLGILKDGDIVSITGGSTMLEFSNVI
                     KTDKRYPNSTIVPARGSMGTDVEMQSNNIVASISKKLHSNCKLLHIPDELDESAMKTL
                     SQVPEIKTTLEYIKRTDILVFSIGRADVMAKRRKLPEDKVDEILSKNAVGEAFGYYFN
                     KDGEIVYKLNTVGIDLETVKQVKETIAIFAGTEKVEALLAISNMHKNMVLVTDEESAY
                     KILSLL"
     gene            complement(21588..22934)
                     /gene="rpoN"
                     /locus_tag="KRL67_004074"
     CDS             complement(21588..22934)
                     /gene="rpoN"
                     /locus_tag="KRL67_004074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HBH1524841.1"
                     /translation="MNFNNSLELTQSQKLIMTTQLKQSLSILNMSKLELEEEIKREAE
                     NNPLVEVEKSGEINWEEYIKDMDRSRRLDRTEISYNPDNEVNLENLVKYSSNLYEDLK
                     FQISLYKLTDKELEVCEYIIDSLDEDGYLRTEEKVIVDTLKIDEELFEKCLISVQQLE
                     PSGVGARSLSECLMIQMASLGIYNEILEEIVTKDLNLIANNKYKEISKKYNMSLQKCV
                     ELINIIKTLDPKPGRTCSVEKSVYIQPDVTVEKIDDEFIVYLNEKDSYQIRINSYYKD
                     ILKNSQSDESAKEFIKERLNSATGLMKNIESRKTTVLKIAEEIVKAQDEFLRKGTKYI
                     KPLKMKDIAEKLEFHESTISRGVNGKYMLTPFGVYEFRYFFSSAIETENNEMASSTSI
                     KKIIKETIKDENKKKPLSDDHISKLLKEKGINVARRTVAKYREELGIPSSSKRKEY"
     gene            complement(23086..25647)
                     /gene="xdh"
                     /locus_tag="KRL67_004075"
     CDS             complement(23086..25647)
                     /gene="xdh"
                     /locus_tag="KRL67_004075"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium-dependent xanthine dehydrogenase"
                     /protein_id="HBH1524842.1"
                     /translation="MVEFILNGEKVVAKEGKKLLDFLREDMNITSVKNGCSEGACGTC
                     MVIIDGKATKACVFKTDKLAGKNITTIEGLSERERDVFAYSFASQGAVQCGFCIPGMV
                     ISSKALIDVNNNPTEDDIKKALKGNICRCTGYVKIIKAVDLAAKIFRENIDVPKVNCK
                     GLVGENLPRVDAVGKALGYSEYVDDMRIEGMIYGSAVRAKYPRALVKKIDIEEAKAYP
                     GVVAVLTAKDIPGTIKVGHLKKDWDTLIPEGEITRYLGDGIVLVAAETREALEEAKKL
                     VKIDYEELTPLSTTADALREDAPKIHETGNILVKEHLVRGNAKEKIENSKYVVTKRYT
                     TPATEHAFLEPECAVAGPYEEDGVIIYSTDQGVFATQHECADMLGLPYEKVRVINKMV
                     GGGFGGKEDMSVQHHAALLAYHTKRFVKVQLTRKESIIVHPKRHATEMEFTTACDENG
                     YLTGMKATIISDTGAYASLGGPVLQRLCTHAAGPYNYQDIDITGTAVYTNNPPGGAFR
                     GFGVTQSCFATECNLNLLAEMVGISPWEIRYKNAIRPGQVLPNGQFADEGTAMAETLE
                     AVKEEYEKHPRAGIACAMKNSGVGVGIPDVGRCRLVIKDGKVHIRTSASCIGQGLGTV
                     MTQVVCETASLLPENVVHEVPDTHLTPDSGNTTASRQTVFTGEATKVASNKLKEALDS
                     GKTLLDLEGEEFYGEYESITDKMGCDKENPVSHVAYGYATQVVMLDENGKLEKVVAAH
                     DVGRAINPISLEGQIEGGVVMGLGYALTEDYPVVNSVPTAKFGTLGLFKSTDIPEIQA
                     LIVEKNKDLLAYGAKGVGEICTVPTAPAVQNAYYVFDKEFRTELPLQNTPYSKKK"
     gene            complement(25641..27032)
                     /gene="hydA"
                     /locus_tag="KRL67_004076"
     CDS             complement(25641..27032)
                     /gene="hydA"
                     /locus_tag="KRL67_004076"
                     /EC_number="3.5.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropyrimidinase"
                     /protein_id="HBH1524843.1"
                     /translation="MGIVLKGGKVVTSEKTYCADVRIEDEKIVDIGFDISKENDEIVD
                     VSDSYVIPGGIDTHTHFDLDTGVTKTADNFETGTKAAIVGGTTTILDFATQAKGCSLK
                     DGLEEWHEKAKDKSYSDYGFHMAITDWNDSVCNEMEDMVKEGVSSFKLYMAYKGSLQV
                     DDGVIFEALRKAEEIGGIIGFHCENGDIICELVDKAKAENNLSPKYHQLTRPAILEAE
                     ATSRLMKIAEVANSKVYVVHLSCKESLEEVLKAKQRGVDVIVETCPQYLLLNDELYDL
                     DGFESAKYVMSPPLRNKENNEILWNAISDNQIDTIGTDHCSFNYKGQKELGINDFSKI
                     PNGSPGVEHRMSLLYTYGVCKNKISINKMVEVTSTNAAKLFGMYPNKGTIEIGSDADI
                     LVLNPNKKSKIEVKKQLQNVDYTPYEGYEVDCQIEHVYLRGKEVVRDGELICNTPSGR
                     YINRRLTKGAREW"
     gene            complement(27311..28420)
                     /locus_tag="KRL67_004077"
     CDS             complement(27311..28420)
                     /locus_tag="KRL67_004077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HBH1524844.1"
                     /translation="MANLKVNLYGMEFLNPIMTAAGPGAKDGALCQEAAKGGAGAICT
                     KTISVLPADVPRPCMAKTNSGFLNTELWSELPKEQWIEEEYKKVKETGLPVIVSMGYS
                     AEQIAEVAPLVKPYADAVELSTHYVGTDVAPIVNALKAAKAVLDVPVFMKMSPHTDIQ
                     TIAKAVEEAGADGLVMINSFGPCMAIDVNTGYPIMGSKAGYGWLSGAPIRPLAIRCIY
                     EASQVVNIPIIGVGGITCGKDVAEMMMAGASAVQVCTEAILKGPSIYGKIAKELNEFL
                     DENGYADVNEIRGLTHKKMAARGFRTHSVAPVVDDNKCIKCKQCVTSCVYEALEVTDK
                     LNIDADKCFGCGLCVTRCPKGALSICYNQVEAMNE"
     gene            complement(28616..29968)
                     /locus_tag="KRL67_004078"
     CDS             complement(28616..29968)
                     /locus_tag="KRL67_004078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine permease"
                     /protein_id="HBH1524845.1"
                     /translation="MSQDKSPKIAPVDERLPVSKSWVFSLQHVLAMCAGAVAVPMMIG
                     NAARLDHASIVFLINACLFMAGIGTLIQSVGIGNIVGAKIPVIEGTSFAAVAGMTAIA
                     SSYSDSQVAIQTVFGSVIVAGLFCILLAPVFEKLIRFFPSVVTGTVVMVIGISLFPVG
                     IKWITDNKLEAVEPNSLALALAVLAITLLSFKFLKGIWNSTAILVGIVLGTLLSMAVG
                     MADFSAVNGVGWLNLNVPLKFGMPRFEPSAILSLCLIMLVLMTESVGNMIAIHEMVDK
                     EVTGKNIKRGLFGDGISTALSGIFNSFPITPFGQNTGIVGLTGIKSRFIAVYAGVILL
                     VLGFLPKFAAIIGAIPKPVLGGVGFAMFGMVMVGGVKTLSKVKFDGTKNGVIVAVSVG
                     LSMIPLTNPDFYHNFPTWVQTIFHSGITTGSLSAVLLNIFFNELGSKKEKNNKVEEAK
                     "
BASE COUNT         9258 a         5455 c         4172 g        11257 t
ORIGIN      
        1 aatatcatca atctatataa aatttaataa cttatataaa tattatcaat ctatataaaa
       61 tttaataatt tatataaata ttatcagtct atataaaatt tagtaattta tataaatatt
      121 atcaatctat ataaaattta ataacttata taaatttaac taatttatat aaaatcttaa
      181 ggagttgtca tgataaaaaa aatagagcat gtggtcatta ctaataataa tatagaaaat
      241 taatataaat tatatatgtc tttttgacta tctttttata tcttcttaaa tgtccgtaaa
      301 gccttatttt atcggctcta cgggcatttt gcttttgttg taaacctcac atatctaggt
      361 ctatcttctt atattttcgc tatcaagcgt ggttaaaatc gtggtaaata cttctatgcg
      421 attagacgct gaacctctga ttttgcagta tctatggacg catgagcata ccagttcatt
      481 gtgatactga tgtttgaatg tcccatgata tactgtaaat cttttgggtt catgttctta
      541 cttgccagcc ttgtgcagaa cgtatggcgt agtgtatgcg gtgtgatatg tggcaaggga
      601 ttgtccttat ggtgcttgtt gtatttctct accatacgga caaaaagcat attgtaatca
      661 atcgcaactt tgggcttgcc tttatgattt acaaatagaa agttgccccg tccgtctatc
      721 acaaatggtt ctgcctttgg gcgtttcttc ataacccgtt gaaatgcttg tatcgtttct
      781 ctgcttaatg gcacttgcct tgctccgctt tttgtcttag gcgtttcaat atagtagccc
      841 tgttccttgc tctttaataa ctggtggtcg ataatcacaa cttcattctt gaaatcaata
      901 tcggctactg tcagtccgca cagttccgag atacgaagtc ctgtctttaa cagtatcagc
      961 acatcatcat aatacttgtg atacacattg tccgccttga tgaatgagag tagggcttgt
     1021 tcctgttcct ctgtcaatgc gactttctct ttggtatcat tttctataac ttcacttaac
     1081 ttgaaatcaa aagggttttt ccttacacaa tcgtcttgta tggcgatata gaatgacgct
     1141 tttaacgagc gtttatggtt gttaatggtg ttataggaaa agcctttgtc tttcatgcgt
     1201 aacgcccatt ccttagcgtc agagggtttt atcgtatcaa tgctcctagc acctaacttg
     1261 tcctctttca ataaccgcat gagctgttcc cgttgtttct gtgtgctctt cttcacgttt
     1321 gccctctgtg tgttctgttt ggcgtagagt tggcaaagcg tcattttctt gcctgtgctg
     1381 tcgataccgt cctcaatatc ccgtcttatc tgctgttcca gttcacgaag tgagggtttt
     1441 tcccgttttc cctttggtgt cgggtctgtg ggtgtcaatc tccaagcata cacatatttt
     1501 gtgtttccaa atgcgtccac atatttatat aagtatttcc cgtctgttcg ttggctctct
     1561 ccagtatgca ggatacgtcc tttgctatcc cgtctttttt ctttcatggt gtctgctcct
     1621 ttccttgttg gaaagagcct tgatatgact tgtattcatc ataacacata caaggctcat
     1681 ttgcattaga ttgcgtccaa tttgtcaatg accttttcaa actgtcggcg tttaatctgt
     1741 atgcggttgc cattcataat gagccagcca gcgtccttgt tttcctctgc caatcgtctt
     1801 aacttgtttt ctccgatacg gaaatatttt gacgcttctt caatggtaag ggtgtatttt
     1861 tcccatacgg gaatatcgtt gttattcatc agataccccc tttcccatgt ttcgtgttat
     1921 attggatata ggggataaga tcggtgcggt ctatcctctg taagtgggcg gttagtttac
     1981 tggaaaggga attgctgtat cttgccttat caagcatagt tcccactttt tccagtccac
     2041 ctaatgcgtc atgttcttcc aagaagtaaa aacttttcac agcggaaatc acgccaccct
     2101 ctgtgagcca ttgctgtgtt tcctcaagag aattatgtag tttcggtact tgcagtttta
     2161 agacttccat agcccctaaa aagcgttccc aaaaccgaca cgttttccat ctgctcttat
     2221 tactttcatt tctgtttggc acgacaaagc gtaggttgtt tgccaatagc ccgaaagcca
     2281 gttccccaag ttccagcggt ctgtctttga atgtcatggc aaaagcatga gccttatcat
     2341 ctcgcagttg catttctgtc cgtttccaac tgccgacttc atcaagtgtc ttattatgtt
     2401 ttgaacagac ttccttatct ttgtcataaa agcggtagga caatcccgat ttaccagcac
     2461 caatataaac agtctttgca gtgtcgaaat catcaaactt gctttcgtca aagtggtagc
     2521 cctcactatt agaaataaat tcctcttttt cgcatttctt ctttatctgc tctatggtaa
     2581 agaatggctt ttcgttctta tcatcaatgg caatatcaag tcttgtgaaa tggaaattat
     2641 ccagtccgta tcttcgctca cagcgtcgga acatatcccc aaaggtataa ttcctactgt
     2701 cgagaatacg gaaaatatca tcacaacctc tgccagtcat aacaagataa cagcctagcc
     2761 cctgtgggtt gtcctctgtc tttcttgcgt cccccgatac ataaatatct ccaatctgcc
     2821 agcgtgcctg ataagtcttg aattttatcg ttgctggata aacattgaaa atgtcagtcg
     2881 gtaaatctaa aatgtgcatg attacatctt cagcggttgc cgtttcaaat acaatgctga
     2941 tgtaatcaat cttaacggat aaatttttgt gtgtagttat agtgcatttc tcctttcgta
     3001 tgccccgttt ttttgcgggg taaaaattgt gttttttcct ttatttatca cagtttctag
     3061 gtactatgac atgtatacgc agggcggtgt tacgcagggc ggtgttacat atacgccctc
     3121 tgcgaacgcc tttaggcgtt ccttttccaa aaggaactgt ctgtccgaac acgtctgggg
     3181 gacgtgcccc gtccagccag ccccttttgg aaaccactat tttcggtctg atagtccatg
     3241 cttattcatg ctatcaaagt gaaaatgtat cttctctatg cttactcaag cgtgtgattg
     3301 accttatcgt gcttaatcac tctatctgat acgataataa cgccattaga attacttgtc
     3361 aagaagtttt tgttgacttt tagatgtaga ttaagtatga tatgcttata aataccaaga
     3421 aaagcaacga aaggagcggt tatactaagt tatggagcat tacgaaaaga aaatcagttt
     3481 cttaggagag aacatacaga ccataagaaa acacagagga atgaaacaac aggaacttgc
     3541 ggataaaatc ggtatcaata tgcagagcct ttccaagatt gaacgaggtg tgaattttcc
     3601 taccttcgat acattagaaa agataatgga cgtgctgggt gtaatgccca atgaattatt
     3661 atcgggagaa tggaagtatg ttaatcaatc cgaaaaggaa gtctgccagt ttttaagaat
     3721 tgaagaacga ttaaatgcag aactaaaaca tggacattat gacaacttct ttgacagcga
     3781 ggaagaatgg ctggaatatg agttggaaaa gttacgggaa tatattaccg actacatcaa
     3841 cgggaaaagt attgtagcgt ccgaccttta tccaatcaaa gaatttatcc agcatttgaa
     3901 gtttcaaaaa ctgttagacc gctatgatga tttatacagc atggatatgt tcggggaaag
     3961 catagaggga cataaatata ggacacctta tcaagtcgta aaaatgataa accccaattc
     4021 aaaagaagat atggaattat tacgggaagt attgaaaaat aaccattttg atgatgagga
     4081 cgaataactt tttccctctt tgtagaagat acagcaaaca aaaagcccag taaagagtgc
     4141 aaagcaccac caatggtggc aaggctcttg acagggcttt ttcttttctg tggagggtaa
     4201 tcaagaggaa gaaaagataa aatttgattt gtggtaaatc ggataatatc aatgaaattg
     4261 tcacggtaca aatttgggct atataaggaa tagtaactgg aagattgtag ggtaatcaaa
     4321 aaggcgtgta ttttaaatca gagatacacg tctttgaaat gaatgaaatt caatgaattt
     4381 tattcattga caatcgttca ttcacgagat ataattttag tgaaaggagt tgattacatg
     4441 aatattacga aacaaaaatc acaaaagact tttgataagc aagctgccga ttatgataca
     4501 aatattcagg gggaacatgc tcgaaaactc tataagccga ttatagaaaa tttaaaaaat
     4561 aagaatatac attctatatt agatttaggt tgtggaacgg gtgcgttact taaagaaata
     4621 aaagaattaa atatagcaga acaactattt ggaatagata tatctcccaa tatgcttgaa
     4681 attgcaaaaa ataaactagg aaatgatgca acacttatct taggcgactc tgagagatta
     4741 ccttttgaag atagttcttt tgacgccatt gtatgtaatg actcatttca tcattatcca
     4801 caaccagata ttgtagagaa agaagtgtct aggtgtttaa agcaaaatgg agttttcatt
     4861 atcggtgatt gttggcaacc aatcggagca agacagataa tgaattacta tatgaaacac
     4921 agcaatagtg gagatgtaaa gatttattca aaaaaggaaa tgttgttatt attatcaaaa
     4981 gacttccacg aaattgaatg gaaatctttt ggcacaagat cttgtttaat tatagcatac
     5041 aataattaaa ccgcaagaaa ggaaagaaaa caatgcgtat ttcaaaagaa ccagaggaac
     5101 gtaaacaaga aattttagaa acagccatta aattatttag tgtaaatggg tttgaaaaaa
     5161 cttcaatatc agacatagca aaagaaattg gaatagcaca aggattatgt tatagatatt
     5221 tcccatcaaa agatgtactt tttcagtctg ctatcaatga atattcaaat attttagtcg
     5281 ccaatatgac aaaaaatata aatataaaaa aggatagttt aaaagatatt ttgcggaaga
     5341 tgatactttt tgcagaacaa gaagatgata catattatcc agtttttcat aataaccaaa
     5401 ataaaaattt ccataattta ttaacattga atgtatgtga aaaacttttt cctattgtat
     5461 ctgaaattat tgaaagagca aattacacaa atgaaataca agtcaatgat gttgaaatgt
     5521 atgccagttt ttgcatatat ggacaactag gaattatttt aaataccgat atatcaatta
     5581 aagaaaagag ttcacggata aaggcatttt ttagggactt gtttaggctt taaatcttcc
     5641 cctgccaaaa ggcagggaat atttattata attcgtccat acgaattacc tcctgcaata
     5701 tagtcagaga ggaaacccct ctacctaata gccacaggag ataatgattg ttgaggattt
     5761 tgaaaaaatt aatgggtatt tggattattg tgcggaattt gtgtatgttg aaatggattt
     5821 aaaattgatt cagcaatttt tctactttaa caaacgccct ttaattgaat aacttacttt
     5881 aaagaaaact ggatatttat gttgaaataa gaacaaaatt gtgaatggtt taacttaaat
     5941 gttaacggta aacggtgcag gttagtttaa ctacatttat atccaaaatt gtttgttttt
     6001 caaattacat gtattttttt gagtttacat agggaaagat ttatggtata ctatttaaca
     6061 cttatattga caagaaagag gtgaaagcaa tgttaactgt ttttagaatt ttgtttggta
     6121 aaaatgcaaa agttggtaat cgttcggcat acaaagggag tagtctgtcc ttttttaagc
     6181 catataacca tacaaaaaca gttatcggag cttttcctct acggcaaaca aaccagagtt
     6241 atatggaaag atgagaaaac atgcaacaaa aaaataagta tataagaatt caagagttct
     6301 tgaagcagaa taaatttcct aattatagaa tgaaacaaat tacaaatgct atattcccag
     6361 ggagaataaa taatttcaac gaaataacgg ttcttcctaa atcactaaga gatatgttaa
     6421 ttgaggagtt tggagaatcg attttaaata ttgttccttt aaaagcacaa caatctacac
     6481 aagtttcaaa agtcttattt ggaatttcag gagacgaaaa aatagaaacg gtaaatatga
     6541 aatataaagc tggttgggag tcattttgta tatcatcgca gtgcggttgt aattttggtt
     6601 gtaaattttg tgcaactgga gatataggtt taaaacgtaa cttaacttca gatgaaatta
     6661 ctgaccagat tttgtacttt cacttacaag ggcattcaat tgacagtatt tcttttatgg
     6721 gaatgggaga agcattagcg aatgtacaag tttttgatgc tttaaatgta cttacagatc
     6781 ctgcgttgtt tgctttaagt ccgcgtaggt tatctatatc cactatagga attattccaa
     6841 acattaaaaa attgactcaa aactatccgc aggtcaacct gacattttca ttacattctc
     6901 cttttaatga acagcgaagt gagttaatgc caattaatga acgctaccca ttatcagatg
     6961 tgatggatac attagatgag catatacgag taacctcaaa aaaagtttat attgcttata
     7021 ttatgttgca cggagttaat gattctattg aacatgcgaa agaagtcgta aaccttttaa
     7081 gaggtagata taggagtggg aacttgtatc atgtgaacat cattagatat aacccgacta
     7141 ttagttcacg gatgcggttt gaagaagcaa atgagaaatg tcttgtcaac ttttataaag
     7201 aattaaagtc agcaggaatt aaagttacca ttagaagtca atttggcatt gatatagacg
     7261 ctgcttgcgg tcaattgtat ggaaactatc aaaaaaccaa tagccagtaa cttgaggtga
     7321 gagtgatgtt tagaacgtgg gaagtaccct aataatttat ttagtaacga taaataaact
     7381 tattaggata tttatgataa aaggaatctt agaaagaaaa ataggcatca ctcaagtgga
     7441 gctaaaaagt cgtttgtaaa aattgaaact gcaattaaat tattgaatgt gaaaaatggt
     7501 gatgattagt gttaaacaaa aaaataattt cagtcaaagt atcagtagag atcatgtagt
     7561 cattacggat ttttggacgt tgactgacaa taatgaaatt caactaccgt ccaacatgga
     7621 aattatatgc aataattatt tgaagagttt gaaattgatg tatcagaaga ttactctaaa
     7681 cagtagttgg aaaaatttgt catgcgagaa ttgcggatgc agtgaattat tgataatcag
     7741 gtggaagaca gtagtgtatc caatgaagag atgaacacga ataccaaaaa tcacttaaaa
     7801 taaggtggat atgcaaaagg aatacaatcg tgttatcctt gatatgaaaa gcaagaaaag
     7861 aaatgggaaa catttgttct caatttaaaa aggcaacgtc aattgaaata tttataattt
     7921 cgcttatcta aaaggcctta gagcatattt taatgaacaa aattgaaagg actgaaccta
     7981 taataatgat tcactaatcc ataaactatt ttgtatttat actttacagc cattttaaga
     8041 agttttggca atactatgtt tcaagcagtt gcaatgcttg tgtctacttt attgaatgct
     8101 attttagatc ctattttcat taacataatt ggatttcacg gagcggcaat cgcaacctta
     8161 atatcacaga ttatttgttt ggcgtttatg tgcatttata tttatcgtaa aaaattattt
     8221 aacctttcac tatctgattt tgatacaaaa gagattatgc cgtttgtaaa aaatgctgtg
     8281 ccgtctgtca ttcaacaaag tattcctgct attagtacaa catttttaac tgctattgtt
     8341 agtacttata gtatttctgc tattgctggc tacggagttg ctggaaaatt agaaatgata
     8401 ttattttatc ctgctatggc tcttaatatg gtgttgactt caatcgtagg gcaatgtatt
     8461 ggagcacaac gtatcgacag agcaaagaat tatctgaaat tggcgttaaa atatggtatt
     8521 gttgtttcaa gtgtattatc agcagttgtt attttcttct ctaaacagtt ggcaaaattg
     8581 tttgtttcca acgtagctgt aacaaatgtt gtcagccatt attttttaat tgttagtatc
     8641 ggttatgttc tttacactat tactagttgc tacttaggta ctttaaatgg tataggaaaa
     8701 ccaacaaaaa gtatgatact aatgatattc tactatatta ttattcgtat tccttttgcc
     8761 tatctattat cttggctggc agaaaattta aatggtgttt ggatagcgat tttaatcagt
     8821 cacatagtcg cctgcattat aactatttta acaagtaatc aaattattaa gcggttatat
     8881 ggaaaacttt atgataggac taaaaaagca agatataaaa gcattgaaag taactgctgt
     8941 ggaaagtttt aggaataatc ttatacgatt atgcactacc taacagcatt ttatcaacaa
     9001 ctaaatattt tacaatatta aaagttcgta cagacggtat attttgtacg gacttttttc
     9061 ttgttcttct ttttgtattt tccaaagata tagacggtat ctccgtttat atatttcagc
     9121 gtatgcggaa aggaggtgag attatgaacc catcttcttt cgagcatatc gttagaatac
     9181 agtttaatgc tttgatgatg actgttatta aatgcacagt aaaaaacaga aacagacagt
     9241 ttgcaagacg ttctaagcgt gaagttttat tctgtgaatt atcagatacg aaaaatgttg
     9301 aatgtgtaac taaggataac tattcttgtg attacatttc gtttgaagtc ttgaatttta
     9361 caattcaaat atcaaatgag aaacttgctg ttgctttata taagttatcg aataaagagc
     9421 gtgatgtaat tctattacgc tattttcaaa gtatgagcga ccaagaaata gccgaattat
     9481 atcatgtatc acgttctgct atttatcgca gaagaagtaa cggattgaag aaactaaaaa
     9541 cagtattgaa agaaaggaat tgaaccaatg aaaaaagaat atggctaccc tacattaaaa
     9601 gtaatatgtc aagcgtctgc tggaaatgaa actgccataa gagagatttt gaagttttat
     9661 gacgcttata tatgtaaatt atgcttgcgt ccgttttacc actctgaaag tggtaaaatc
     9721 attatgcagg ttgatgaaga attaaaaggt caaatccata cagaaatgat gaaagcaatc
     9781 ttgaaatttg aaatcagagt aaaataatca tactacatga atgaaaaacg ggagatacac
     9841 agatatttgt gctatctccc atgttgtttt tgtgtcatat caaggctcat tcaatcgtgg
     9901 taaattcgtg gtaaaatgaa tagttttcta tacttcaaaa tgtttgaaag tatagtgttt
     9961 atgcggataa tcaaaaatca gatataaaat ttatatcaat ttttataaaa aatttggctt
    10021 ttcacctaaa gaaatgagtg aaaggtataa acttaatgct agaaatgtta agataaatct
    10081 taacataaaa tgtcataaat ttttacctta tgttaaaaat ttacaagcaa gtagtactaa
    10141 cttatgcttt aaaataaaca caaatattga tgagccaaaa aaatacttag aacaaaatga
    10201 gattcaaatt gagcgaggaa ttgtagaatg tacaggagtc tttagtaata tgaaatctat
    10261 ttatttgaaa caaactaatg gaaatttaat tgaattatca aatcatagaa tcacggagag
    10321 taaaccttgg aaaatttaat cctatcaata aacgttgttc ttcctttgtt tctcacaatg
    10381 tcacttggat atgttctaaa aaaactaggt ctatttgata ctcatacttt aaataaaatg
    10441 aataatgtct gtttcaagtc tttcttgcca ttattattat tttttaatat atacaataca
    10501 gatttagatg gttctcttaa ttttaagctt atggctgtag ctgttataat tattttttca
    10561 atcttcttaa tcttagtctt tattattcct aaaattgaaa aagataataa gacaaggggt
    10621 gcattaatac aaggtgtatt tcgtagcaat ttcgttatat ttggtattcc tgtgacaatt
    10681 tcactatttg gcgataagag tattggagtt acatctttat taattgcagt tattgtacct
    10741 atgtttaatg tattgtctgt tgtaatactt gaaattttta gaggtggcaa agtaagtata
    10801 aaacatatga ttaaaagtgt cttaacaaat ccactcataa tcgcctcttt tataggattg
    10861 acaatgctaa ttttaaatat taatatacct acatttttac aaaaaactat tggtgatatt
    10921 tctaaaatag ctactccttt atcacttata attctaggcg gctcttttag tttttctgct
    10981 ataaaggggc atataaagcc cattatattg ggggtatgtg gaaaacttat acttgtacct
    11041 gctatatttt taccaatcag catttttctt ggatttagaa atgtagaatt agcttcactt
    11101 atgataatgt tatctgctcc aactgcagtt tcttcattca caatggcaca acagatggat
    11161 tctgacagtg aattagctgg acaaattgtg gtatttactt ctgcattttc tataattaca
    11221 gtatttttaa taatatttat tttaaagcag aataatctta tttaatgata tttgataaca
    11281 caaattggta ttatgaaaaa catatataaa attgatttag ttgcgtaaaa acacattatt
    11341 atactatttt tactatataa tataacttta tgtatattat ctcatgaaat atggtgaaag
    11401 tactgttgct gtttattttt tagtttaaaa aaatatatag atacaaacaa tagatgctaa
    11461 cagatagtca atctataaaa taaaaactgg actaatcata taaaatcatt agtccagctt
    11521 ttattttacg tactttttat gttgatgtac ttttattaaa aactaaattt aatttttaaa
    11581 tgtctagcaa atgcactaat taaataatgg ccagttattc aacaatatca ctacttctta
    11641 actataagta atgccaatgc atttataaaa aataatactg ctgttatctt tgtaactcta
    11701 gcaagaaatg cttgttttcc ttttggttta aagtaagctt gacttccttc tccaccaaac
    11761 tctgaaggta aagcattctt tgaatcttga ggcattatgc taattactag taaaatccct
    11821 agtactactt gcacacccat taaaatgttg ctcattcttg cccctcctat ctgtttatta
    11881 cttaaatttc attactcttt agtgtatcac aataatatag ccatttcaag actattatgc
    11941 tataaatcac atataaaaaa atatataatt actgtatctt ttttgagtaa cgtatttcta
    12001 ataatttttt cccaatatat atctctttct caaaatcatc acaagtaaaa ccattttttt
    12061 tgtaaaattt tatagctcta gtattatctt ttaagaccca tattgttata tacgaatatc
    12121 ctaaatttct tagtctatca atacaaaatt gtaatccttt ttttgtaaca ggagttcccc
    12181 aaaaatctgg atggaaatat atagagtata tttctccaat atattttgga gcattttctt
    12241 catgactttt atttaatgaa gcaaaaccag caggtatacc atccacttta aaaagatagt
    12301 attcctcttc agcttttgga ataacattgt gaaaaatttc tgctcttttt tcagaagtga
    12361 actcatctat tatattatct ggaataattc ctttatacgc ttttttccat gaataagaat
    12421 ggataaaacc catttctgta gcattttcac ctgtagctcg tataatctct aaattcataa
    12481 aaaccaccat cctattgaca aattaatacg actatcgtat tatattagta cgataatcgt
    12541 atcataattg gaggtaaatg tcaatgaatg ctataaaaga actaagcaac ctatggcaca
    12601 tcgttggtat gcaaatgaaa acagaaatgt gtagtggtaa gtatcctact atttatggta
    12661 tttctttagt agaattaagt attttagaaa tagttgaaca acacccaaat agtatgatga
    12721 aagaaattag ttcaacttta gaattaccaa aaagcacatt aacaagtgct gtaaaacgtc
    12781 ttgaattaaa aaaacttata aagagaaatc cttgcattaa agataaaagg gcgtataatt
    12841 tagatttaac tgaattaggt ttaaaagctc agcaagaaca tagagaaata gaagcaactg
    12901 tatttaaaaa tatcctacat caattagata aagatgagat agaaacattt attcatattt
    12961 tttctaaagc tgtgaataaa attaggtaat aaaatataaa aaggataagt gaattgaaaa
    13021 atcaactaac acttatcctt atataagaca aacttaaacc ttttatacta tctctttaat
    13081 cttaataact atttttttaa gttatagaaa gattttaatc cacagtatct agcttgctct
    13141 ccaaccattt cttctattct taataattgg ttgtacttag caactctatc agttcttgat
    13201 ggagcaccag tttttatttg accagcattt actgcaacag ctaaatctgc tatagtagaa
    13261 tcttcagtct ctcctgatct atgagatata acagcagtat atcctgctct cttagccatt
    13321 tctatagcat ctaaagtttc agttattgta cctatttggt taactttaac taatatagag
    13381 ttagctactc cattttcaat tcctttttct aatctctctg tgttagtaac aaataagtcg
    13441 tcaccaacta attgtaattt atttcctaat ttttcagtta ataatttcca tccatcccaa
    13501 tcttcttcgt ctaaaccatc ttctatagtt attattggga agttgtttga ccaatcttca
    13561 tataaagcaa ccatttcagc agcagttaac tcttttcctt ctcctgctaa aacgtatttt
    13621 ttagtttctt tgttgtacat ttctgtagca gcaacgtcaa gacctaacat aacgtcttca
    13681 cctggcttat atccagcttt tgttatagct tcaactatta attctaaagc ttctctgttt
    13741 gaacctaagt ttggagcaaa tccaccttcg tcacctacac cacaagctaa tcctttttct
    13801 cctaaaactt tctttaatga atggaatact tctgctccca ttcttaaacc ttctttgaaa
    13861 caacaagctc ctactggtaa tatcataaat tcttgaacat caacgttgtt atcagcatgc
    13921 tctccaccgt ttaatatgtt catcattgga acaggtaatt gtttagcatt tactccacct
    13981 aaatattgga ataatggaag acctatttca tcagcagctg ctctagctac tgccattgaa
    14041 acacctaata tagcattagc tcctaattta cctttgtttg gagttccatc caattctatc
    14101 atgattgcgt ctatagctgg ttggtcaaat gcatccattc cttctatttc aggtgctatt
    14161 atttcattta cgttagcaac agctgtttct acaccttttc ctaagtatct tcccttatct
    14221 ccatctctta attcaacagc ttcaaaagct cctgtagatg ctcctgatgg aactattgct
    14281 cttcccattg ctccatcttc taatactact tcaacttcta cagttggatt tcctcttgag
    14341 tctaatactt ctctagcata tactaattca ataactgaca tttgtatatc tcccttcgat
    14401 tttctattag ttaaattttc atttactgta aacatccgtc tactatttta atctactttc
    14461 tatattatag tacataaagt tgattttgaa aattaggtac aaatttattt gatgggtaaa
    14521 tttgtaaaag ttcatatttt tattttattt tttataatat tttaatttat ataatataaa
    14581 aaaacttgtc taaaatccaa aaccaatctt aaatacacaa atattataac tagatttaaa
    14641 acattaaaca taaaatgtac aactaatgat tctaaaattt ttgcttatat aatatatatt
    14701 aattattatt atttatattg ttattattta tattgttatt atttatatca ttatttacat
    14761 tattatctat attataattt tttattttat aaattgtcta tcttgtaaat tttataaaat
    14821 atactttcat ctacacctta caatatccag accttacaaa atctaaatct tatgtcataa
    14881 agtctaaacc ttacaaaata aacctaaact gtgttatttt taaatttata ttgtagttta
    14941 tatattattc tctacttatc agaatcgact attttgaaat aagtgaatga ccagtcattt
    15001 cttctggctt ttctaacttc atcatatcta aaacagtcgg tgctatatca gataatcttc
    15061 ctccatccaa taattttgca ctttcatatt cttgaccaac tacaataaat ggtactggat
    15121 taatagagtg agctgttaca gtttttcctg tttctgggtc taacatatat tcagcatttc
    15181 cgtggtcagc agttattatt gcacttccac ctaactctac tattttatct accaattttc
    15241 ctacacaagt gtctacagtt tcaacagcct ttacagccgc ttctatactt cctgtgtgac
    15301 ctaccatatc aggatttgca aagtttaaca ctataaagtc aaacttatct tctcctaatt
    15361 tctctaaagc tttatctgtc aattcatatg ctgacatttc tggttttaaa tcataagttg
    15421 caacctttgg agaaggtatc aatagtcttt cttcaccttt atttggctct tctactccac
    15481 cattgaagaa gaaagttaca tgtgcatact tttcagtttc agcagctcta agttgagttt
    15541 ttccattttt agctagatac tcacctagag tgttggctaa agattgaggt ccaaatgcta
    15601 tatgcacatt ctctatagtt atatcgtact ctgttaagca tacaaagaag cttttaatat
    15661 cttctctttt aaatccatca aactcttcac aaactaaagc tctagttatt tgtctagctc
    15721 tatctggtct aaagttgaag aatataattg aatcattttc ctttatactt ccaactggtt
    15781 tatcatcttt cataataact gttggcatta taaactcatc attttttcca tcatcataag
    15841 agttttgaat agcttcttca atagaatttg ctttttcacc aatacctcta accatagcat
    15901 tgtatgcaag ctctactctt tcccatctct tatctctatc catagcgtag tatctaccag
    15961 aaactgtagc aaattcacct actccaattt catccatact agcttgtact tctttggcat
    16021 attctaatgc actttgaggg tcagtatctc taccatcagt aaatgcgtga acatacacct
    16081 tttgtacgcc tttatctttt gacattttga ttatagcctt aagatggtct atatgtgaat
    16141 gtactcctcc atcagacaaa agtcccatta catgaagaga attttctttt gcattatcca
    16201 ttgcttcaca taaaacttta ttagtaaaaa agtcaccatc ttttatagat tttgttattc
    16261 tagttaaatc ttgatatact attctacctg cacctatgtt agtgtgtcca acttctgaat
    16321 ttcccatttg accatcaggt agaccaacat caagaccact agcatttatc aatgtgtttg
    16381 gatactcttt ttttattcta tctaaattag gagttttgga ctcagcaatt gcatttccct
    16441 taacatcctt attgtatcca aacccgtcca taataattaa agcaactggt tttttcatca
    16501 tttcactcct tagaaattaa caaggtctag gtaatcatta ctagctaaac tagctccacc
    16561 tactaaagct ccatctatat cactttgacc cattatttcg gctacatttg aaggtttaac
    16621 acttccaccg tattgtattc taacttcatt agctaattct ccatataatc cttttataac
    16681 ttctcttata taagatataa cgtcattagc atcttcagca gttgcagttt taccagttcc
    16741 aatagcccag attggctcat atgcaacaac tacttttgct aaatcatcct taagaacatt
    16801 ttctaaagct ttttcaactt gaattttaca aacgtcttta gtttttccag cttctctttg
    16861 ctctaaagtt tctccaacac ataatattgg gtctattcct acttctaatg cttttaaaac
    16921 tttcttgttt acagtttcat cagtttcatt gaagtattgt cttctttctg aatgtcctat
    16981 tacaacatag tccatatcta tttcttttaa cataagaggt gaaacttctc ctgtaaatgc
    17041 tcctttttct tcaaagtgca tattttgagc acctatcttt atatttgtac ccttagtagc
    17101 ttcttttaaa tcctttaata aagtaaatgg tgcacatata acagcttcta ctttatcaga
    17161 atttacttta tatttaactt catttacaaa ttctaaagct tcttttattg ttttatgcat
    17221 tttccagttt cctgcaatta taggttttct cattttacaa tctcctctat atattagttt
    17281 atttattatc taatgctgca atacctggta attctttacc ttctaaaaat tctagtgatg
    17341 ctcctccacc tgttgatacg tgagtcatct tatcaccaaa tcctaattga ttaactgctg
    17401 ctgcactatc tccaccacca attacagtag ttgcatcagt taactctgcc atagctctag
    17461 ctactgacaa tgtaccattt gcaaagtttt caaattcaaa tactcccatt ggtccattcc
    17521 atactacagt ttttgattct tttatagttt ttacaaagtt tgctatagtc ttaggtccca
    17581 tatctagtcc catgtaatct tcttttacat tagcatcttc tgttactatt ggagtagcat
    17641 ctggtgcaaa gtggtctgcc ataacaacgt ctactggtaa taataagtta acacccttat
    17701 tcttagcttt ttccatcatt tctttagcat attcaacttt atctatttca agtaaagaag
    17761 ttcctacttc ataaccctga gcttttaaga atgtataagc cattcctcca cctattatta
    17821 gattatcaac tttttctaat aattcgttta taacagctaa tttatcagaa actttagctc
    17881 ctccaagtat agctgtaaat ggtcttacag ggtttgcaac agcttctcct aagaatttta
    17941 attctttttg aattaagtat ccacaaactc tctcttgtaa gaactcacct gcaccaactg
    18001 ttgagcaatg tgctctatga gcagttccaa acgcatcatt tacaaatatt tcagcaagtg
    18061 atgctaattc tttagagaaa ttctcttcgt ttttagtttc ttcttttcta tatctagtat
    18121 tttctaataa tactacatct ccgttttcca ttttttcagt tgctttcttt gcattttctc
    18181 ctacaacatt atcgtctgct gcaaatacaa cttcttttcc taacatctca gataatctct
    18241 ttgctactgg tgctaaagat aattctggct tagcttctcc ttttggcttt cctaagtgag
    18301 agcataatat aacttttgca cctttagaaa ttaaatattg aatagttggt aaagctccgt
    18361 tcaatctatt ttcatcagta attacaccat cttgtaatgg cacattaaag tcacatctta
    18421 ctaatacttt ttttccgcac acatcaatat cttctatagt ttttttgtta agcattgaca
    18481 taaatatctc tccttttaat aaagtaattt tttattgcca agtataatat agtacatact
    18541 tttaccctat taaatatatc atattatcat atttttatat atagtatgta acattattta
    18601 ggtgtatttt atatattttt tcttaaaaaa agtcctgagt ccgtagtatt aaactacaaa
    18661 ctcggacttt gtcaatctac ttttctattt tctcaaaact gactattttg ctaattgtgc
    18721 aaagtatcct aaagttctta ttaattgaga agtataagac atttcattat cataccaaga
    18781 aacaacttta actaattgtt ttccatctac ttccataact tttgtttgag tagcgtcaaa
    18841 taatgaaccg tagcttattc cgattatgtc agaagatact aacatttctt cagtatatcc
    18901 gaaagattcg tttgaagctt ctttcatagc tgcatttatt tcttcaactg taacattttt
    18961 tcctaatgta caaactaatt cagttattga accagttaca actggtactc tttgtgctgc
    19021 cccatctaat tttcctttta atgaaggtat tactaagcct attgcttttg cagctcctgt
    19081 agtgtttgga actatgtttc cagcagcagc tctagctctt cttaaatcac cttttggatg
    19141 aggaccatct aaagtatttt ggtcattagt gtatgcatgt atagtagtca ttaatccttt
    19201 ttctataccg tatttatcat ttaatacttt agccattgga gctaaacagt tagtagtaca
    19261 agaagctcct gatataactg tttcagaacc atctaatata tcactgtttg tgttgaatac
    19321 tattgttttt aaatctccag tagctggtgc agatataaca actttcttag cccctgcttg
    19381 tatatgagct tcagctttat ctttagatgt aaagaatcct gtacattcta atactatatc
    19441 tactcctaat tcagcccatg gtaggtcagc aggatttctt tctgctgtta cttttatttc
    19501 ttttccgttt actacaaaag caccttcttt aacttctatt tctccgttaa atcttccttg
    19561 agctgtatca tatttaaata aatgagctaa cattttagcg tctgttaagt cgtttattgc
    19621 aacaacttcg aacttatctt gttgctccat catttttctt aatgctaatc ttcctattct
    19681 tccaaatcca tttatagcta ctttaaccat tttcgctacc tcctataaag taaatgtatg
    19741 ttttctagct ctgtgctagg tcttgacttt tataataatg acaatatctt ataagcactt
    19801 tcttcatctg ttacaagaac catgttttta tgcatatttg atatagctaa taaggcttct
    19861 actttttcag tacctgcaaa gattgcaata gtttctttta cttgtttaac tgtttctaaa
    19921 tcaattccaa cagtgttcag tttgtaaact atttctccat ctttgttgaa ataatatcca
    19981 aatgcttctc caactgcatt tttagataat atctcatcaa ccttgtcttc tggaagtttt
    20041 cttctctttg ccatgacatc tgctctacct atactaaaaa ctaaaatgtc tgttctttta
    20101 atatactcca aagttgtctt aatctctggc acttggctaa gagttttcat tgcactttca
    20161 tccaactcat ctggtatatg taaaagtttg caattagaat gtaatttctt acttattgaa
    20221 gcaactatat tattagattg catctctaca tcagttccca tacttcctct agctggaact
    20281 attgtagagt ttgggtatct cttatcagtt ttaataacgt ttgaaaactc taacatagtg
    20341 cttcctcctg ttattgaaac gatatctcca tcttttaaaa tacctaagaa atactcgcat
    20401 ccatatctag ttacatcttt tagtaaactt ccattttcat cacagcttcc aggaactaat
    20461 attacccttt ttattccaag cttttctctg actctatctt gtaacttaga caatcccatg
    20521 atgtcactca tgatgtcctt aagtttatct aacaaatcag aaccttcttt agtgatagtc
    20581 atcccagata cagctacgtc tataagtcct tgttctttta aaaactcagt ttctgacctg
    20641 attattcttt cactaatctc taatacattt gataatgttc tcctacctat tggttcactt
    20701 aaagatattt gtcttaaaat agagtatctt ctttccataa gttcaattgc ctgtggaatc
    20761 aatttctttt gaatttctaa taagttcttc ataacatact ccttattgct ttcctttcgg
    20821 gacttttttc gtccctagtg tttatttaag tcccacaaga tatttttatt ttataggatt
    20881 tgcgtataga tttcaatagt ttttcaaaaa cttttttatt tttttatttt ataaaatatt
    20941 tctccaccat tttcaaatca cagtattttt tctttttata taaggctttt tatatatatt
    21001 tttttatatt ttcattctaa tatttattac tataaataat tttatttcta gttactttta
    21061 tttttagtta tttttatcta caactaagtt tacttttatc tagttaagat ttttatttat
    21121 accctgtgtt ttccttttgt gattattaaa tttaataata gtttatttat ctaggtattc
    21181 ttctttttct aatatctaat tattctttac aaacacttat ttaatttaat cattctttat
    21241 aagcacttat ttaattatgt tttctagttt tttagcattt tacaatattt taatgttccc
    21301 cagtttttca attcttttta gtatttttat attttataaa ttttaatatt ctttaaagtt
    21361 ctaatattcc tcaaagctct aatgttcttt aatgttttaa tcttctttag ttttttatgt
    21421 tctttgctat tttaatgttt taatgttccc cagtttttta atatcttata gcattttaat
    21481 acttttccat attctattat tttatttcat aaaattttta gcctaatcca cattcatctt
    21541 ttaattagcc ttttcccctt acaatactga tatttgtctt ttacttatta gtattctttt
    21601 ctcttacttg aagatgggat tcctaattct tcacgatatt tggcaactgt tcttcttgca
    21661 acattgattc ctttttcttt tagtaactta gatatatggt catcacttaa aggtttcttt
    21721 ttattttcgt cttttattgt ttcctttata atttttttta tgcttgtact agatgccatt
    21781 tcattattct cagtctcaat ggcgctactg aaaaagtacc taaattcata tacgccaaaa
    21841 ggtgttagca tatatttacc atttacacct ctacttatag tagattcatg aaattctaat
    21901 ttttctgcta tgtctttcat tttcaaaggt tttatatatt ttgtcccttt tctcaaaaat
    21961 tcatcttgag cttttactat ctcttctgca atcttaagta ctgtagtttt cctactctct
    22021 atatttttca taagtcctgt tgctgaattt agtctttctt ttataaattc ttttgcactt
    22081 tcatcagact gtgaattttt taatatgtct ttataataac tattgattct tatttgataa
    22141 gaatcttttt catttaaata tacaataaat tcatcatcaa ttttttctac tgttacatca
    22201 ggttgaatgt atacactttt ctctactgaa caagttcttc ctggttttgg gtctaatgtc
    22261 ttaattatgt ttattagttc aacacatttt tgtaatgaca tattgtactt tttacttatc
    22321 tctttgtatt tattatttgc aattaaattt aagtcttttg taacgatttc ctctaaaatt
    22381 tcattatata ttcctaaact agccatttgt atcatcagac attctgaaag acttcttgcc
    22441 cctactccac taggttccaa ctgctgaaca ctaattaaac atttttcaaa aagttcttca
    22501 tctatcttta aggtatcaac aattactttt tcctcagttc ttaaatatcc atcctcatct
    22561 agactatcaa ttatatactc acagacttcc aattctttgt ctgtaagttt atacaggctt
    22621 atctgaaatt ttaaatcttc atataaattt gacgaatatt taactaaatt ttctagatta
    22681 acttcattat caggattata gcttatctct gttctatcta aacgtcttga cctatccatg
    22741 tcttttatat actcttccca atttatctcc ccacttttct cgacttcaac taatgggtta
    22801 ttctctgctt ctctttttat ctcttcttct aactctaact tactcatatt taatattgat
    22861 aatgattgct taagttgtgt agtcattatt agcttctgag actgagttaa ttctagacta
    22921 ttattaaaat tcattctgct cacctctcaa ttttattaaa cttattttat aaaagaaaaa
    22981 ccctcctgta tatgtatatt ctatcattta aaatatacct tatgcaaaag ggtttttttt
    23041 atttacaatt tcatctattt ttttatttaa tatttctttt ttttgttact tttttttact
    23101 atatggagta ttttgtagtg gtagttcagt tctaaattct ttatcaaata cataataggc
    23161 attttgtact gctggtgcag taggaactgt acaaatttct cctacacctt ttgccccata
    23221 agccaataaa tctttgttct tttcaactat taaagcttgt atttctggta tatctgtaga
    23281 tttaaataaa cctaatgtac caaacttagc tgtaggaaca gagttaacta ctgggtaatc
    23341 ttcggttaga gcatatccaa gacccataac tactccacct tctatttgac cctctaaact
    23401 gattggattt atagctcttc caacatcatg tgctgctact actttttcca attttccatt
    23461 ttcatccagc attacaactt gtgttgcata tccataagca acatgactta ctggattttc
    23521 tttgtcacaa cccattttat ctgtaatact ttcatattct ccataaaact cttctccctc
    23581 taaatcaagt aaagttttgc cagagtctaa agcttctttt aacttatttg acgcaacctt
    23641 agtagcttct cccgtaaata ctgtctgtct tgaagccgta gtattaccag agtctggtgt
    23701 taaatgagta tctggaactt catgcactac attttcagga agtaggctag cagtttcaca
    23761 aactacttga gtcataacag taccaagccc ttgacctata caagaagcgc ttgttcttat
    23821 gtgaactttg ccatctttta tcaccaatct acatcttcca acatcaggta taccaactcc
    23881 aaccccacta tttttcatag cacaagctat acctgcacgt ggatgctttt catactcttc
    23941 ttttacagct tctagtgtct ctgccattgc tgttccttca tctgcaaatt gaccatttgg
    24001 taaaacttga ccaggtctta ttgcattttt atatcttatc tcccaaggag atattccaac
    24061 catttcagca agtaaattta gattacattc agttgcaaaa caactctgtg taactccaaa
    24121 tcctctaaat gctcctccag gagggttatt agtatatact gctgttcctg ttatatcaat
    24181 atcttgataa ttgtatggtc ctgctgcatg agtacacagt ctttgtagta ctggtcctcc
    24241 aagagaggca tatgcacctg tgtcagatat tatcgttgcc ttcatacctg ttaagtatcc
    24301 attttcatca catgctgtcg taaattccat ttctgtggca tgtcgttttg gatgaacaat
    24361 aatactttct tttcttgtta actgtacttt tacaaatctc ttagtatggt atgcaagtaa
    24421 agctgcatgg tgttgaacac tcatgtcttc ttttccacca aatcctcctc ctaccatttt
    24481 gtttataact cttaccttct catatggtag ccctaacata tctgcacatt catgttgagt
    24541 ggcaaacacc ccttggtctg ttgaatagat tattactcca tcctcttcat aaggaccagc
    24601 tactgcacat tcaggctcta aaaatgcgtg ttctgtagca ggtgtagtat atcttttagt
    24661 tacaacatat tttgaatttt ctatcttttc tttagcgttt cctcttacta aatgttcctt
    24721 aacaagtata ttacctgtct cgtgtatctt tggagcatct tctctaagtg catctgctgt
    24781 agtacttaat ggtgttaatt cctcataatc tatcttaact agcttttttg cctcttctaa
    24841 cgcttctcta gtttctgctg ctactaaaac tattccatct cctaagtatc tagttatttc
    24901 accttctggt attaaagtat cccaatcctt ctttaagtgc cctaccttta tagttcctgg
    24961 aatatcttta gctgttagaa ctgcaacaac tcctggataa gcttttgctt cttctatatc
    25021 tattttttta actaaagctc taggatactt agctctaact gctgaaccat aaatcatacc
    25081 ttcaattctc atatcatcta catattcaga atatccaagt gctttgccaa ctgcatcaac
    25141 tctaggaaga ttttcaccta ctagaccttt acagtttact tttggcacat ctatattttc
    25201 tctaaaaatc tttgctgcta aatcgacagc tttaattatt tttacatacc ctgtacatct
    25261 acatatatta ccttttaaag ctttctttat atcatcttca gtaggattat tattaacgtc
    25321 tataagtgct tttgaactaa ttaccatacc tggaatacaa aatccacatt gtacagctcc
    25381 ttgactagca aaagaatatg caaaaacatc tctttctctc tctgataatc cctctatagt
    25441 tgttatgttt ttcccagcta atttatcagt tttaaataca catgctttag tagctttacc
    25501 atctattata accatacatg ttccacaagc accttctgaa catccatttt taactgaagt
    25561 tatattcata tcttctctaa ggaagtctaa aagttttttg ccttcttttg caacaacttt
    25621 ctctccattt aatataaatt ctaccattct ctagccccct ttgttagacg cctatttata
    25681 tatctcccac ttggagtatt acagattaat tctccatctc taactacttc ttttcctctt
    25741 aaataaacat gttctatttg acaatccact tcataccctt cataaggtgt ataatctaca
    25801 ttttgtaatt gttttttcac ttctattttg gattttttgt ttggatttaa gactaatata
    25861 tctgcatcac tgcctatttc tatagttcct ttattaggat acattccaaa caactttgct
    25921 gcattagttg atgttacttc taccatcttg ttgatagata ttttattttt acagactcca
    25981 tatgtgtata aaagactcat cctatgttct acacctggag aaccatttgg tatcttactg
    26041 aaatcgttta ttccaagttc cttctgtccc ttataattaa aagaacaatg gtctgttcct
    26101 atagtgtcta tttgattatc tgaaatagca ttccataata tttcattatt ttctttattt
    26161 cttaaaggtg gtgacattac atacttagca ctttcaaatc catctaaatc atataattca
    26221 tcatttaata gaaggtattg aggacaagtt tcaactatta catccacacc tctttgctta
    26281 gcttttaaaa cctcctctaa ggattcttta caacttaagt gaactacata tactttagag
    26341 tttgcaactt cagctatctt cataagtctt gacgtagctt ctgcctccag tatagctggt
    26401 ctagttaatt gatgatattt tggagataaa ttattttctg cctttgcttt atccacaagt
    26461 tcacatatta tatccccatt ttcacaatgg aaaccaataa tcccacctat ctcttctgct
    26521 tttctcaatg cttcaaatat gacaccatca tcaacttgaa ggcttccttt atacgccata
    26581 tacagtttaa atgaagatac tccctctttc accatatcct ccatttcatt acaaaccgag
    26641 tcattccaat ctgttatagc catatgaaag ccataatctg aataggattt atccttagct
    26701 ttttcatgcc attcttctaa tccgtctttc aaagaacatc ctttagcttg tgtagcaaaa
    26761 tccaatatag tagtagttcc tcctactata gcagcttttg ttcctgtctc aaagttatct
    26821 gctgtcttag taacgcctgt atctaagtca aagtgtgtgt gtgtatctat acctcctggt
    26881 ataacataag aatcactaac atcaactatt tcatcatttt cttttgatat atcaaaacct
    26941 atatctacta ttttttcatc ttctattctt acatctgcac agtaagtttt ttcacttgtt
    27001 actactttcc caccttttaa aacaattccc atatagactc caccttactt gtaatgtatc
    27061 aatttatttt atatgaaaaa attaacttaa agtaatctag ttagttcact tatacgtagt
    27121 aaatctatat taagattaaa ccaataccta attaaattaa tattcagcta aactaatatt
    27181 tagttaaatc aatatcataa aattattaac tttagactgt attattaatg cataatgcgt
    27241 gtttatctga tatattttat tatcctaata cctattactc taagttaatt accaaaattc
    27301 tatataatta ttactcattc atagcttcta cttgattgta gcagatgctt aaagctcctt
    27361 ttggacatct agttacacat aatccacatc caaaacactt gtctgcatct atgttcaact
    27421 tatctgtaac ttctaaagct tcgtatacac agcttgttac acattgctta catttgatgc
    27481 acttgttatc atccactact ggagctactg aatgagttct aaatcctcta gctgccattt
    27541 tcttatgagt taatcctctt atttcattaa catcagcata tccattctca tctaaaaatt
    27601 catttaattc tttagcaatt ttcccataga tagaaggtcc tttaaggatt gcttctgtac
    27661 atacttgaac agcacttgct ccagccatca tcatttcagc tacgtctttt ccacaagtga
    27721 ttccaccaac accgattatt ggtatattta caacttgaga agcttcgtat atacatctta
    27781 ttgctagagg tcttattgga gcacctgata accatccgta tccagcttta cttcccatta
    27841 tagggtatcc tgtatttaca tctattgcca tacatggtcc gaatgagttt atcattacaa
    27901 gcccatctgc acctgcttct tcaacagctt ttgcaatagt ttgaatatca gtatgaggac
    27961 tcattttcat aaatactggt acatctaaaa cagcttttgc agcttttaaa gcatttacta
    28021 ttggtgcaac atctgtacct acataatgag tagataattc aactgcatct gcataaggtt
    28081 ttactaaagg agcaacctca gctatttgct ctgctgaata tcccatactt actattactg
    28141 gaagacctgt ttcttttacc tttttgtatt cttcctctat ccattgctct tttggaagct
    28201 ctgaccataa ttctgtgttt aaaaaaccac tattagtttt tgccatacat ggtcttggta
    28261 catcagctgg taatacggat atagtttttg tacatatagc tcctgctcca ccttttgctg
    28321 cctcttgaca gagagcacca tcttttgctc caggacctgc cgctgtcata attgggttta
    28381 aaaattccat tccatataaa ttaactttta aattagccat attattatct ctcccttaaa
    28441 ttaaatattt ttaatattta gattgtattc ttttatttgt gttcgttatt tatttacttt
    28501 tttatattat ttaaaatata agatagtttt gttgaattat taatatctct gaattatttt
    28561 tattaatgtt taataacttt taatcaatat agatttttac ttacttgttt tatttttact
    28621 ttgcttcttc aactttatta tttttttctt ttttagaacc tagttcattg aagaatatgt
    28681 ttaaaagcac tgctgataaa ctacctgttg ttattccact atggaatatt gtttgtaccc
    28741 aagttgggaa attatgatag aaatcaggat ttgttagtgg tatcatagac aaaccaacac
    28801 taactgctac tataacccca tttttagttc catcaaattt cactttgctt aaagttttta
    28861 ctcctccaac cattaccatt ccaaacattg caaatcctac gcctccaaga acaggcttag
    28921 gtattgctcc tattattgct gcaaattttg gtaagaatcc aagtactagc aatataaccc
    28981 ctgcgtaaac tgctataaat cttgatttta tacctgtaag acctactatt cctgtatttt
    29041 gaccgaatgg agttattgga aatgaattga atattccaga taatgctgta gatattccat
    29101 ctccaaatag acctctttta atatttttac ctgttacttc tttgtctacc atttcatgaa
    29161 tagctatcat attaccaact gattccgtca taagtaccaa cataatcaaa cacaatgata
    29221 gtattgcaga tggttcaaat ctaggcattc caaattttaa aggtacattt aagttaagcc
    29281 aaccaactcc attcactgca ctaaaatctg ccattccaac tgccatggaa agtagtgtac
    29341 caagtactat acctaccaat atagctgtgc tattccaaat tccttttaag aatttaaatg
    29401 aaagtaaagt tattgccaat actgctaaag ctaatgctaa tgagtttggt tcaaccgctt
    29461 ccaatttgtt gtcagttatc cattttatcc caactggaaa tagtgaaata cctattacca
    29521 taacaactgt tccagtaact acactaggga aaaatcttat taatttttca aatactggag
    29581 caagtaatat acaaaacaat ccagctacta ttacagaacc aaatactgtc tgtattgcaa
    29641 cttgtgaatc actatatgat gaggctattg ctgtcatacc tgccacagcg gcaaaactag
    29701 ttccttctat tactggtatt ttagcaccta caatatttcc tataccaaca ctttgtatga
    29761 gtgttcctat acctgccata aacaagcaag catttattaa aaaaacaata ctagcatgat
    29821 ctagtcgtgc agcatttcct atcatcatag gaactgctac tgctcctgca cacattgcta
    29881 aaacgtgctg tagtgaaaat acccaagatt tggatacagg taacctttca tctactggag
    29941 ctattttagg gcttttatct tgagacattt tgggtctccc ttctatattt aatttaatat
    30001 tctattccgt atatttttta tttatatact tcatttaaat tcctattttt gtttaatctc
    30061 tgcttttaac atctaaattc ttgtttctta cttgtatatt aatctgaacc tcccatgact
    30121 aaagtcacag ggttcctgct tc
//
LOCUS       DAEPXF010000423        23293 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain HB27
            SAMN13376389-rid12479423.guided.052, whole genome shotgun sequence.
ACCESSION   DAEPXF010000423 DAEPXF010000000
VERSION     DAEPXF010000423.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13376389
            Sequence Read Archive: SRR10518432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 23293)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23293)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001069624.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001069624.1
            Genome Coverage       :: 315x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/15/2021 01:54:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,165
            CDSs (total)                      :: 4,076
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 89
            rRNAs                             :: 10, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 10 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 19 of 69
            Pseudo Genes (incomplete)         :: 44 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 10 of 69
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23293
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13376389-rid12479423.guided.052"
                     /strain="HB27"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="China: Hebei"
                     /collection_date="2017"
     gene            672..3722
                     /locus_tag="KRL67_004079"
     CDS             672..3722
                     /locus_tag="KRL67_004079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SrtB-anchored collagen-binding adhesin"
                     /protein_id="HBH1524846.1"
                     /translation="MKKMLKRLCTGFLALATVVTALPTTPVHAESKQYWTESAERVGI
                     IEKVMNDGSIGSTFNEGYMKVEGETAYCIDINTNFKNGYKTRADASSRMSADQISDVA
                     LSLEYVKQYGEAHKELNYKQVYLLEQCVVWQRLSVHFGWQCDNVRASYDEIPKATQDE
                     VFSGAKAFIKENKGRYECGGYIYSGEGQELGQFWAKLNVGNAKLQKTSSNTSITDGNG
                     NYSIAGATYGVFSDKDCTKQLATLTTDENGNTDVVEVKAGTVYIKELSAPAGYKVDKT
                     IYSLKVEVGKTAILKVSDTPKVTDTLIELFKIDMETQKDNPQGNASLAGAEFTWKYYA
                     GFYNKDNLPAEATRTWVTKTIAETDSDGTTHYIAKLADAYKVSGDSFYMQDGKAVLPL
                     GTLTVEETKAPNGYLLDGAYMQAGDKSEQIKGLYVTQITEDGDLAVLTGSNQFSVSDK
                     VIRGGVKIQKRDLETGDTKPQGSATLKDTAFDIISLNDNAVLVEGKLYKKNEVVKTIH
                     TDIEGVASTSSDLLPYGKFRIVESEAPDGYLTDGAKPIDFTITENGKIVDLTDEAHSI
                     YNQIKRGDIEGVKIGVGTHKRLADVPFRITSKTTGENHVVVTDDNGQFSTSADWASHK
                     HNTNAGKTSEDGVWFGTSEPDDSKGALPYDTYIIEELRSDSNKGFELIPPFEIVVSRN
                     NLVIDLGTLTDEYEKEISIHTTATSKDGEKTILAGKEITIVDTVKLDGLTKGTKYQLK
                     GWQMLKEENAELLIDGKRVENDYTFVADDEEMKVEISYTFNASALGGKNLVTFEELYD
                     LSNPDEPVKVAEHKDIEDDGQTVLITERIVKIHTTATDKDGNKELEAGKDVTIIDTVI
                     LEGLEVGTQYKLVGWQMLKEENAELLINGKRVKSDYTFTADSETMKVEVAFTFDATSL
                     DGKQLVTFEELYDLSNPDEPKKVTEHKDIEDKGQTITFKEKTKVPEEPKKPETPQTPD
                     TPHKTDSPKTGDSTNLYGLLALLLTSGAGLAGIFFCKRRKMKKS"
     gene            3959..4285
                     /locus_tag="KRL67_004080"
     CDS             3959..4285
                     /locus_tag="KRL67_004080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HBH1524847.1"
                     /translation="MEMKYVVPDMAQSFGTLEFAGESEPVFERDKNNRRVLARRSYNL
                     YSDVQKGENVVVEIPVQAGEKHFKYEQKVKLVNPKLYGRGYAIGDMGHTDYVLLADDI
                     VAVEEK"
     gene            4301..4684
                     /locus_tag="KRL67_004081"
     CDS             4301..4684
                     /locus_tag="KRL67_004081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009588269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HBH1524848.1"
                     /translation="MRLSNGFVIDKEKTFGELKFTAVRDVFLQNEDGTPSTQLKKRIY
                     DLKCSLHGGIIPVSVPPEVPLREFPYNAVVELVNPVADTVSRKTYTGADVDWYVKAED
                     IVLKNKSNQNAGNTQNHTPQGQPKK"
     gene            4786..5550
                     /locus_tag="KRL67_004082"
     CDS             4786..5550
                     /locus_tag="KRL67_004082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008687118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HBH1524849.1"
                     /translation="MDSWFTVEQIDQDTFVISEYKHWEETHCYLLCGTKRAILIDTGL
                     GVSNIREVIDGLTKLPVTVITTHVHWDHIGGHKYFRSIAVHEAEKEWLSVKFPIPLQV
                     VKNNIMCNPCDFPSDFKVEKYQIFQGVPQMILHDEDCIDLGNRKLVVIHTPGHSPGHC
                     CFYEADRKYLYSGDLIYSGCLDAFYPSTNPQEFWKSVRKIQSLKISRILPGHHHLSIP
                     VTIIDKIETAFHNLSNEGKLKQGNGIFSYEGFQIHI"
     gene            5649..7043
                     /locus_tag="KRL67_004083"
     CDS             5649..7043
                     /locus_tag="KRL67_004083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008787614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBH1524850.1"
                     /translation="MRMIWNKGHRIRASDKHLVYHFSIGTLLFVFVAVLLLLNSKQLM
                     CTDWEHFSLLENGLTLSPYNFITILIATGVCALVAFLYYRFCYDSFKKLLHRQKLARM
                     VLENKWYEADTVQDSIFFTDLQSRSREKIVWFPKIYYQMEKGLLHIRCEITLGKYQDQ
                     LLRLEDKLESGLYCELTDKTLHDGYIEYTLLYDMIANRITIDEVRAENGCLRLMKNLV
                     WEYDALPHALIAGGTGGGKTYFLLTLIEALLHTNAVLYILDPKNADLADLGTVMGNVY
                     HTKEEMIDCVNAFYEGMVQRSEEMKRHPDYKTGENYAYLGLPPCFLIFDEYVAFFEML
                     GTKESVSLLSQLKKIVMLGRQAGYFLIVACQRPDAKYFSDGIRDNFNFRVGLGRISEL
                     GYGMLFGSDVKKQFFQKRIKGRGYCDVGTSVISEFYTPLVPKGHDFLQTIGPLAQARQ
                     DVTATCEAKGDGTD"
     gene            7013..7198
                     /locus_tag="KRL67_004084"
                     /pseudo
     CDS             7013..7198
                     /locus_tag="KRL67_004084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002907732.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
     gene            7221..8426
                     /locus_tag="KRL67_004085"
     CDS             7221..8426
                     /locus_tag="KRL67_004085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000398284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HBH1524851.1"
                     /translation="MEGFLLNEQTWLQHLKEKRLAYGLSQNRLAVATGITRQYLSDIE
                     TGKVKPSEDLQQSLWEALERFNPDAPLEMLFDYVRIRFPTTDVQQVVENILQLKLSYF
                     LHEDYGFYSYSEHYALGDIFVLCSHELDKGVLVELKGRGCRQFESYLLAQQRSWYEFF
                     MDVLVAGGVMKRLDLAINDKTGILNIPVLTEKCQQEECISVFRSFKSYRSGELVRKEE
                     KECMGNTLYIGSLQSEVYFCIYEKDYEQYKKNDIPIEDAEVKNRFEIRLKNERAYYAV
                     RDLLVYDNPEHTAFKIINRYIRFVDKDDSKPRSDWKLNEEWAWFIGNNRERLKLTTKP
                     EPYSFQRTLNWLSHQVAPTLKVAIKLDEINQTQVVKDILDHAKLTDRHKQILKQQSVK
                     EQDVITTKK"
     gene            8469..8690
                     /locus_tag="KRL67_004086"
     CDS             8469..8690
                     /locus_tag="KRL67_004086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002570387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH1524852.1"
                     /translation="MNFGQNLYNWFLSNAQSLVLLAIVVIGLYLGFKREFSKLIGFLI
                     IAIIAVGLVFNAAGVKDILLELFNRIIGA"
     gene            8807..9304
                     /locus_tag="KRL67_004087"
     CDS             8807..9304
                     /locus_tag="KRL67_004087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000342537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HBH1524853.1"
                     /translation="MDDMQVYIANLGKYNEGELVGAWFTFPIDFEEVKEKIGLNDEYE
                     EYAIHDYELPFTVDEYTSIGELNRLWEMVSELPEELQSELSALLTHFSSIEELSEHQE
                     DIIIHSDCDDMYDVARYYIEETGALGEVPASLQNYIDYQAYGRDLDLSGTFISTNHGI
                     FEIVY"
     gene            9279..9785
                     /locus_tag="KRL67_004088"
     CDS             9279..9785
                     /locus_tag="KRL67_004088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016897981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HBH1524854.1"
                     /translation="MGFLKSSIKSVGTLLLADFLFYGVAQSATPIFYERIDYMKKIRS
                     YTSIWSVEKVLYSINDFRLPFPITFTQMTWFVVSLFAVMILGNLPPLSMIEGAFLKYF
                     GIPVAFTWFMSTKTFDGKKPYGFLKSVIAYALRPKLTYAGKKVTLGRNQPQEAITAVR
                     SEFYGISN"
     gene            9769..12216
                     /locus_tag="KRL67_004089"
     CDS             9769..12216
                     /locus_tag="KRL67_004089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBH1524855.1"
                     /translation="MAYPIKYIENNLVWNKDGECYAYYELVPYNYSFLSPEQKIQVHD
                     SFRQLIAQNRDGKIHALQISTESSIRSAQERSKNEVTGKLKAVAYDKIDQQTDALISM
                     IGENQVNYRFFIGFKLLLNDQEFSMKSLTVEAKNALSDFVYDVNHKLMGDFVSMSNDE
                     ILRFQKMEKLLENKISRRFKIRRLDKDDFGYLIEHLYGQTGTAYEEYEYHLSKKKLDN
                     ETLIKYYDLIKPTRCLVEEKQRYLKIQQEDETVYVAYFTINSIVGELDFPSSEIFYYQ
                     QQQFTFPIDTSMNVEIVANRKALSTVRNKKKELKDLDNHAWQSDNETSSNVAEALESV
                     NELETNLDQSKESMYKLSYVVRVSANDLDELKRRCNEVKDFYDDLSVKLVRPFGDMLG
                     LHEEFLPASKRYMNDYIQYVTSDFLAGLGFGATQMLGENEGIYVGYSLDTGRNVYLKP
                     ALASQGVKGSVTNALASAFVGSLGGGKSFANNLIVYYAVLYGAQAVIVDPKAERGRWK
                     ETLPEISHEINIVTLTSDEKNKGLLDPYVIMKNPKDSESLAIDILTFLTGISSRDGER
                     FPILRKAIRAVTNSEVRGLMKVIEELRVENTPLSTSIADHIESFTDYDFAHLLFSNGY
                     VEQSISLEKQLNIIQVADLVLPDKETSFEEYTTMELLSVAMLIVISTFALDFIHTDRS
                     IFKIVDLDEAWSFLQVAQGKTLSMKLVRAGRAMNAGVYFVTQNTDDLLDEKLKNNLGL
                     KFAFRSTDLNEIKKTLAFFGVDPEDENNQKRLRDLENGQCLISDLYGRVGVIQFHPVF
                     EELLHAFDTRPPVRKEV"
     gene            12219..14396
                     /locus_tag="KRL67_004090"
     CDS             12219..14396
                     /locus_tag="KRL67_004090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HBH1524856.1"
                     /translation="MKPSIVNRIKSNWTLKRLGKVAMTVAFTLVIAIFLLAMLGTVVQ
                     AAGLVDDTVNVANEYSRYPLENYQLDFYVDNSWGWLPWNWSDGIGKQVMYGLYAITNF
                     IWTISLYVSNATGYLVQEAYSLDFISATADSIGKNMQTLAGVSANGFSTEGFYVGFLL
                     LLILVLGVYVAYTGLIKRETTKAIHAIMNFVLVFILSASFIAYAPDYIKKINDFSSDI
                     SNASLSLGTKIVMPHSDSQGKDSVDLIRDSLFSIQVQQPWLLLQYNSSDIESIGIDRV
                     ESLLSTSPDSNNGEDREKIVAEEIEDRSNTNLTITKTINRLGTVFFLFVFNIGISIFV
                     FLLTGIMIFSQVLFIIYAMFLPVSFILSMIPSFDGMSKRAITKLFNTILTRAGITLII
                     TTAFSISTMLYTLSAGYPFFLIAFLQIVTFAGIYFKLGDLMSMFSLQSNDSQSVGSRV
                     MRKPRMLMHAHMHRLQRKLGRSMTTLGAGSAIVTGKKGQSGSGSSARTQADHSRPDGK
                     EKSTLGKRIGQTIGTVADTKDRMVDTASGLKEQVKDLPTNARYAVYQGKSKVKENVRD
                     LTSSISQTKADRASGRKEQQEQRRKTIAKRRSEMEQVKQKKQPASSVHERPTTRQEQY
                     HDEQTSKQSNIQTSYKESQQAKQERPAVKSDFSSPKVERQGNTVQEKTVQKPATSTTT
                     ADRTSQRPITKERPSTVQRVPLQNTRSRPPIKTATIKKVGKKP"
     gene            14393..15394
                     /locus_tag="KRL67_004091"
     CDS             14393..15394
                     /locus_tag="KRL67_004091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018368130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysozyme/C40 family peptidase"
                     /protein_id="HBH1524857.1"
                     /translation="MKLKTLVIGGSGLFLMVFSLLLFVAILFSDEQDSGISNIHYGGV
                     NVSAEVLAHKPMVEKYAKEYGVEEYVNILLAIIQVESGGTAEDVMQSSESLGLPPNSL
                     STEESIKQGVKYFSELLASSERLSVDLESVIQSYNYGGGFLGYVANRGNKYTFELAQS
                     FSKEYSGGEKVSYPNPIAIPINGGWRYNYGNMFYVQLVTQYLVTTEFDDDTVQAIMDE
                     ALKYEGWRYVYGGASPTTSFDCSGLTQWTYGKAGINLPRTAQQQYDVTQHIPLSEAQA
                     GDLVFFHSTYNAGSYITHVGIYLGNNRMFHAGDPIGYADLTSPYWQQHLVGAGRIKQ"
     gene            15391..16326
                     /locus_tag="KRL67_004092"
     CDS             15391..16326
                     /locus_tag="KRL67_004092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002989471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HBH1524858.1"
                     /translation="MRKEDLMMKFRKNQNKEKQIPKEKKPRVYYKVNPHKKVVIALWV
                     LLGLSFSFAIFKHFTAIDTHTIHETTIIEKEYVDTHHVENFVENFAKVYYSWEQSDKS
                     IDNRMESLKGYLTDELQALNVDTVRKDIPVSSSVRGFQIWTVEPTGDNEFNVTYSVDQ
                     LITEGENTKTVHSAYIVSVYVDGSGNMVLVKNPTITNIPKKSSYKPKAIESEGTVDSI
                     TTNEINEFLTTFFKLYPTATASELSYYVNDGILKPIGKEYIFQELVNPIHNRKDNQVT
                     VSLTVEYIDQQTKATQVSQFDLVLEKNGSNWKIIE"
     gene            16569..16685
                     /locus_tag="KRL67_004093"
     CDS             16569..16685
                     /locus_tag="KRL67_004093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance determinant leader
                     peptide"
                     /protein_id="HBH1524859.1"
                     /translation="MILKYPENICMLCMPMVMHKNPSDKSIYHWDFYALLGF"
     gene            16701..18620
                     /gene="tet(M)"
                     /locus_tag="KRL67_004094"
     CDS             16701..18620
                     /gene="tet(M)"
                     /locus_tag="KRL67_004094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011117014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance ribosomal protection
                     protein Tet(M)"
                     /protein_id="HBH1524860.1"
                     /translation="MKIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTD
                     NTLLERQRGITIQTGITSFQWENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAK
                     DGVQAQTRILFHALRKMGIPTIFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVEL
                     YPNMCVTNFTESEQWDTVIEGNDDLLEKYMSGKSLEALELEQEESIRFQNCSLFPLYH
                     GSAKSNIGIDNLIEVITNKFYSSTHRGPSELCGNVFKIEYTKKRQRLAYIRLYSGVLH
                     LRDSVRVSEKEKIKVTEMYTSINGELCKIDRAYSGEIVILQNEFLKLNSVLGDTKLLP
                     QRKKIENPHPLLQTTVEPSKPEQREMLLDALLEISDSDPLLRYYVDSTTHEIILSFLG
                     KVQMEVISALLQEKYHVEIELKEPTVIYMERPLKNAEYTIHIEVPPNPFWASIGLSVS
                     PLPLGSGMQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYS
                     PVSTPADFRMLAPIVLEQVLKKAGTELLEPYLSFKIYAPQEYLSRAYNDAPKYCANIV
                     DTQLKNNEVILSGEIPARCIQEYRSDLTFFTNGRSVCLTELKGYHVTTGEPVCQPRRP
                     NSRIDKVRYMFNKIT"
     gene            18739..18906
                     /locus_tag="KRL67_004095"
     CDS             18739..18906
                     /locus_tag="KRL67_004095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich KTR domain-containing protein"
                     /protein_id="HBH1524861.1"
                     /translation="MCPVCGNKTRLKIREDTELKKFPLYCPKCRQENLIEIKQFKVTV
                     ITEPDAKTQSR"
     gene            complement(18966..19319)
                     /locus_tag="KRL67_004096"
     CDS             complement(18966..19319)
                     /locus_tag="KRL67_004096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBH1524862.1"
                     /translation="MRKKEDKYDFRAFGLAIKEARLKRGLTREQVGALIEIDPRYLTN
                     IENKGQHPSIQVLYDLVSLLHVSVDEFFLPANNLVKSTRRLQIEKYMDSFTDKELSLM
                     ESLASGINEARNIED"
     gene            19824..20246
                     /locus_tag="KRL67_004097"
     CDS             19824..20246
                     /locus_tag="KRL67_004097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006733729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HBH1524863.1"
                     /translation="MKPSSFQTTIENQFDYICKRAMEDERKNYMLYLSRIAKREVSFS
                     DVGDYLVSQFATTDNYSTDFQIFTLNGLSVGVENDLLSEALRELPDKKREILLLFYFM
                     DMSDSEIADLLKLNRSTVYRHRTSGLALIKKFMEEFEE"
     gene            20243..20473
                     /locus_tag="KRL67_004098"
     CDS             20243..20473
                     /locus_tag="KRL67_004098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002411750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBH1524864.1"
                     /translation="MKTQYPMIPFPLIVKATDGDTEAINQILHHYRGYITKRSLRLMK
                     DEYGNQSMVVDEVLRGRMETRLITKILSFEIK"
     gene            20934..21137
                     /locus_tag="KRL67_004099"
     CDS             20934..21137
                     /locus_tag="KRL67_004099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006730246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HBH1524865.1"
                     /translation="MKQTDIPIWERYTLTIEEASKYFRIGENKLRRLAEENKNANWLI
                     MNGNRIQIKRKQFEKIIDTLDAI"
     gene            21219..22436
                     /locus_tag="KRL67_004100"
     CDS             21219..22436
                     /locus_tag="KRL67_004100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020220015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HBH1524866.1"
                     /translation="MSEKRRDNKGRILKTGESQRKDGRYLYKYIDSFGEPQFVYSWKL
                     VATDRVPAGKRDCISLREKIAELQKDIHDGIDVVGKKMTLCQLYAKQNAQRPKVRKNT
                     ETGRKYLMDILKKDKLGVRSIDSIKPSDAKEWAIRMSENGYAYQTINNYKRSLKASFY
                     IAIQDDCVRKNPFDFQLKAVLDDDTVPKTVLTEEQEEKLLAFAKADKTYSKNYDEILI
                     LLKTGLRISEFGGLTLPDLDFENRLVNIDHQLLRDTEIGYYIETPKTKSGERQVPMVE
                     EAYQAFKRVLANRKNDKRVEIDGYSDFLFLNRKNYPKVASDYNGMMKGLVKKYNKYNE
                     DKLPHITPHSLRHTFCTNYANAGMNPKALQYIMGHANIAMTLNYYAHATFDSAMAEMK
                     RLNKEKQQERLVA"
     gene            22661..23224
                     /gene="bclA1"
                     /locus_tag="KRL67_004101"
                     /pseudo
     CDS             22661..23224
                     /gene="bclA1"
                     /locus_tag="KRL67_004101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888190.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-like exosporium glycoprotein BclA1"
BASE COUNT         7685 a         4160 c         4923 g         6525 t
ORIGIN      
        1 aaaaaatatt gttatggtaa tatattctat ttacaatttt ataactttgg tttacaataa
       61 tagagacaat aaatcctaaa aaacataata agaaaaaagt gcttaatatt ttagaaagac
      121 tggtttagtt tacaccagct aagccagtca aggaataatt taaagtgtaa tatataataa
      181 tgaaaaagaa catgctatta gaagtttcta atgacatgtt ctttttttgt agaaatcagc
      241 attattatat aaaaatttaa gactgtaaat tttaattgtg ttgtaactaa gtatgtattt
      301 taaacatagt ataatataga cccaaaatgg agggattata aatgagaaat attatacttt
      361 atttaaatga tgatactttt atatctaaaa acacaataaa aatctgatta tccgctaaaa
      421 cacagtatct atcagcattt tcaagaaaac aaatacatca atttactact tatttactac
      481 taacgaatga gccttgatac gcaagttttc ctagatatgc aaagatatgg tacagcacat
      541 cagtattaaa ataagaaaaa attgaataac tcatagacta tggaacgcct aacatgacgt
      601 tccttttcta tttccagccg ttcttattgg acggctattt tattacccaa aatgaaagga
      661 gcattagatt tatgaaaaag atgttaaaac gattgtgtac gggcttctta gctcttgcaa
      721 ctgtcgttac tgctttaccg actacacccg tccatgcaga aagtaagcaa tactggacgg
      781 aaagtgcaga gcgtgtcggt atcattgaaa aagtaatgaa tgacggttct atcggttcga
      841 cattcaatga gggctatatg aaagtcgagg gcgaaactgc ctattgtatc gacattaaca
      901 caaactttaa gaacggttac aagaccagag ctgacgcaag ctcacgcatg agtgctgacc
      961 agatttcaga tgtggcgtta tccttagagt atgtcaaaca gtatggcgaa gctcacaagg
     1021 aactgaacta caaacaagtc tatctgttgg aacaatgtgt agtctggcag agattgagcg
     1081 tacattttgg ctggcaatgt gataacgtgc gagcttctta tgatgaaatt ccaaaggcaa
     1141 cacaggacga agttttctct ggtgcaaaag cctttatcaa agaaaataaa ggacgctatg
     1201 aatgtggcgg ttatatctac tctggcgagg ggcaggaatt gggacaattc tgggcgaagc
     1261 tgaatgtcgg aaatgcaaaa ctgcaaaaga cttccagtaa taccagcatt accgacggta
     1321 acgggaatta ctctattgca ggtgcaacct atggtgtctt ttctgataag gactgtacga
     1381 aacagcttgc cacccttacg actgatgaaa acggaaatac agatgttgta gaggtaaagg
     1441 ctggcacagt ttatatcaag gagttatccg caccagcagg atataaagta gataaaacca
     1501 tatattcctt aaaggttgaa gttggaaaga cagcaatttt gaaagtatca gatacgccaa
     1561 aagtaacgga cactttgatt gagcttttca agattgatat ggaaacacag aaagacaatc
     1621 cgcaagggaa cgcttcttta gcaggtgcgg aatttacatg gaagtattat gcaggcttct
     1681 ataataaaga caatctccct gccgaagcta ctcgtacatg ggttacaaag acaatcgctg
     1741 aaacagacag cgacgggaca actcactaca tcgcaaaatt agcggacgca tacaaggtat
     1801 ctggcgatag cttctatatg caggacggca aggcggttct tccacttgga acgctaaccg
     1861 ttgaggaaac gaaagctcca aacggctact tgttagacgg tgcatatatg caggctggcg
     1921 ataagtccga acagataaaa ggcttatatg taacacagat tacagaggac ggcgaccttg
     1981 ccgtacttac tggaagtaac cagttttctg tatcagacaa ggttatccgt ggtggtgtga
     2041 aaattcagaa acgagattta gaaacgggcg atacaaagcc acaaggaagt gctaccttga
     2101 aagatactgc ttttgacatc atttccttaa atgataatgc ggtattggtt gagggcaaat
     2161 tatacaagaa aaatgaagtg gtaaaaacaa tccataccga tattgagggt gtcgcttcta
     2221 cttcttctga ccttttacct tatggaaaat tccgtatcgt tgaaagtgaa gctcccgacg
     2281 gttacttaac tgacggtgca aagccgattg attttacaat cacagaaaat ggaaaaatcg
     2341 tggacttaac cgacgaagct cattctatct ataatcagat taagcgtgga gatattgagg
     2401 gtgtaaaaat cggtgtaggt acacacaagc gtcttgctga tgttccgttt cggatcacaa
     2461 gcaagacgac gggcgaaaat catgtagtgg taactgatga taacgggcaa ttctccactt
     2521 ctgctgactg ggcttctcat aagcacaata ccaacgcagg caagaccagc gaggacggtg
     2581 tgtggtttgg aacttctgaa ccagacgaca gcaagggtgc attaccttat gatacctaca
     2641 tcattgaaga attacgctct gatagtaaca aaggctttga acttatccca ccttttgaaa
     2701 tcgtggtatc aagaaataac cttgtgattg atttaggaac gctgactgat gaatacgaaa
     2761 aagaaatttc tatccatacc acagcgacca gcaaggacgg cgaaaaaact atccttgcag
     2821 gaaaagagat tacgattgtt gatactgtaa aattggacgg acttacaaaa ggcacaaaat
     2881 atcagctcaa aggctggcag atgttaaagg aagaaaacgc agaacttctt attgacggga
     2941 aacgtgtaga aaacgattat acctttgtcg ctgatgatga ggaaatgaaa gtggaaattt
     3001 cctatacatt caatgcgtct gctttaggtg gcaaaaacct tgttaccttt gaagaattgt
     3061 atgatttaag caatccagac gaacccgtaa aagttgcgga acataaagac attgaggacg
     3121 acggacagac ggtacttatc acagagcgta tcgtcaaaat tcatacgact gctacggata
     3181 aggacggcaa caaagagctt gaagctggaa aagacgttac aatcattgat accgtaatct
     3241 tagagggctt agaagtcggt acacagtaca aacttgtggg ctggcagatg ttgaaagaag
     3301 aaaatgcaga acttcttatc aatggaaaac gtgtgaaaag tgattacact tttactgctg
     3361 acagcgaaac tatgaaagtg gaagttgcct ttacctttga cgctacttct cttgacggca
     3421 aacagcttgt aacttttgaa gaattgtacg atttaagcaa tccagacgag ccgaagaaag
     3481 ttaccgagca taaggatatt gaggataagg gacagacgat tacctttaag gaaaaaacca
     3541 aagtcccaga agaaccaaag aaacccgaaa caccacagac accagataca ccgcataaaa
     3601 cagacagtcc aaaaactggc gatagcacaa acctttacgg actgcttgca cttcttttga
     3661 cttctggtgc tggactggca ggtatcttct tctgcaaacg ccgtaaaatg aagaaatcat
     3721 aaggcggtaa cggtatgaga aaagaaaaat cacgttctcc gccgataccg tgtcggcaga
     3781 cttccgctta ttctggattg tctgctactt acctgcaaga acacggatag tcaagggtgc
     3841 gtaagcattg attttaccct ttactatctg ggggattgct ggtacttccc aaaccgccag
     3901 aataagaaat cacaatcaaa aaacttatca aaaatagaaa gaaatgaggt aaaacactat
     3961 ggaaatgaaa tatgtcgtgc cagatatggc acagtctttt ggaactcttg aatttgcagg
     4021 cgaaagtgag ccagtctttg aaagagataa aaataaccgc agggtcttag ccagacgaag
     4081 ctataacctt tattctgacg tacaaaaagg cgaaaatgtt gtggtagaaa ttcccgtgca
     4141 ggctggcgaa aagcatttca agtatgaaca gaaagtaaaa cttgtcaatc cgaagttata
     4201 tggcagaggt tacgcaatcg gggatatggg acataccgat tatgtgttgc ttgctgatga
     4261 tattgtagca gttgaagaaa agtaaaggag tgaagaattt atgagattat caaacgggtt
     4321 tgttattgac aaagagaaaa catttggaga attaaaattt acagcggtgc gagatgtgtt
     4381 cttgcagaat gaggacggga caccgagtac ccagcttaaa aagcgtatct atgatttgaa
     4441 gtgcagtctg catggtggaa ttatccccgt gtccgtaccg ccagaagtac cgttaaggga
     4501 atttccgtac aatgcggttg tggagcttgt caatccagta gccgatacgg tatcaagaaa
     4561 aacctatacg ggtgcagatg tggactggta tgtaaaggca gaggatattg tattgaagaa
     4621 taaaagcaac cagaacgcag gcaatacaca gaaccataca ccgcagggac aaccgaaaaa
     4681 atagacttgc tgaatagatt ttcgttgtag aggatatacg gaactgttat aatatgggta
     4741 tgaagaaagc gacaaattaa aatttttagg gagtgagaat agtatatgga cagttggttt
     4801 accgtcgaac aaatagacca agatacattc gtcatcagcg aatacaaaca ctgggaagaa
     4861 acacattgtt atctattgtg tgggacaaaa agagccattc tgattgatac aggtttaggt
     4921 gtttccaaca tcagagaggt tattgatgga ttgacgaaac tgcccgttac agtaatcact
     4981 actcatgttc attgggatca tatcggtgga cataaatatt ttcggtctat tgcagttcat
     5041 gaagcagaaa aagaatggct atcggtcaaa ttccctatac ctttgcaagt ggtaaaaaac
     5101 aatattatgt gcaatccatg tgatttcccc tcggatttta aggtagagaa atatcagatt
     5161 tttcaagggg ttccgcaaat gattttacat gatgaagatt gtattgactt agggaacaga
     5221 aagttggtag ttattcacac gcccggtcat tctcccggac attgttgttt ctatgaagcg
     5281 gatagaaaat atctgtattc cggcgatttg atatatagcg gttgccttga tgctttttat
     5341 ccatcaacaa acccacaaga attttggaag tctgttagaa aaattcaatc tttaaagata
     5401 agtcgaattt tgccaggaca tcatcattta tctattccag ttactataat tgacaagata
     5461 gagacagctt ttcacaactt gtcaaatgaa gggaaattaa aacaagggaa tggtatattc
     5521 agctatgagg ggtttcaaat ccatatctga aatgcaatct tagttttaca attttatatc
     5581 catacacaca aagcagttag tcttaggatt aactgttttt ttcataccca gaaaggagtg
     5641 attgatttat gcgtatgatt tggaacaaag gacaccgtat cagagccagc gacaagcacc
     5701 ttgtctacca cttttccata gggacgcttc tgtttgtgtt cgtggcggtt ctcctgctac
     5761 tgaatagcaa acagctcatg tgtaccgatt gggagcattt cagcttgtta gagaacggct
     5821 tgacgctttc cccttacaac ttcataacca tactgatagc gactggtgtt tgtgcattgg
     5881 tcgcttttct gtattaccgc ttctgttatg acagtttcaa gaagctcctg caccgtcaaa
     5941 agctggcaag aatggtactg gaaaataagt ggtatgaagc tgataccgta caagacagca
     6001 ttttttttac tgacctgcaa agcagatcaa gggaaaaaat cgtctggttt ccaaagattt
     6061 attatcaaat ggaaaaggga ctgcttcata tccgctgtga aattacgctg gggaaatatc
     6121 aagaccagct tttacggtta gaggataaat tggaaagtgg cttgtattgt gagctgaccg
     6181 acaagaccct gcatgacggc tatatcgaat ataccctgct ttatgatatg atagcaaacc
     6241 gcattaccat tgatgaagta cgggcagaaa acggctgtct tagactgatg aaaaatcttg
     6301 tgtgggaata tgacgcactc cctcacgccc ttatcgctgg tggaactggt ggcggtaaaa
     6361 catacttttt gctgacactc attgaagcct tactgcatac caacgctgtc ctttacatct
     6421 tagacccgaa aaatgcggac ttagcagact tagggacagt tatgggaaat gtgtatcata
     6481 ccaaagaaga aatgattgat tgcgtcaatg ccttttatga gggaatggta cagcgaagcg
     6541 aggaaatgaa gcgacaccca gattataaga cgggcgaaaa ctacgcctat ctgggactgc
     6601 caccctgctt tcttatcttt gatgaatatg tggcgttttt tgaaatgctg gggacaaaag
     6661 aaagcgtgag cttacttagc cagttaaaga aaatcgtcat gctggggcga caagcaggtt
     6721 attttcttat tgttgcctgc cagcgtccag acgcaaagta tttctcggac ggtatcagag
     6781 ataacttcaa tttccgtgtg ggacttgggc gtatcagcga attaggttat ggtatgctgt
     6841 tcggttcgga tgtgaaaaag cagtttttcc agaagcgtat caaagggcgt ggctattgtg
     6901 atgtagggac aagtgttatc agcgaatttt acacgccttt agtcccgaaa ggacatgatt
     6961 ttttgcagac tatcggccct cttgcacaag caaggcagga cgtgacggcg acgtgcgaag
     7021 cgaaaggcga cggcacggac tagccgttgc tggtgtggcg taagccacgc cagcaggtaa
     7081 aacccctcgg tatctaacag gggggtacaa atcgacagga aacagtcaaa aaaacattag
     7141 aaaatccttt ggttacaagg gatttacaaa atttcagcgt atgtcaaatg ggctttaaaa
     7201 gttgacatac gcctttttga ttggagggat ttttactgaa tgaacaaact tggttacagc
     7261 atttaaaaga aaaacgcttg gcttatggac tatctcaaaa ccgtttagct gttgcgactg
     7321 gtattacaag gcagtatcta agcgatattg aaacaggaaa agtcaagcca tcagaggatt
     7381 tacagcagtc cctttgggaa gctctggaac gcttcaatcc cgacgctccc cttgaaatgc
     7441 tgtttgatta tgtaaggatt cgctttccga caacagacgt acagcaggtg gtcgaaaaca
     7501 tcttacaact gaaactgtcc tattttcttc atgaggacta tggtttctat tcttattcag
     7561 agcattatgc tttaggcgac atattcgtcc tttgctccca tgaactggac aaaggagttc
     7621 tggtggaatt gaaaggtcgt gggtgcagac aatttgaaag ctatcttctg gcacaacaaa
     7681 gaagctggta tgagttcttt atggacgttt tggtggctgg cggtgtgatg aaacgccttg
     7741 accttgccat taacgataag acagggattt tgaatatccc tgtactcact gaaaagtgcc
     7801 aacaggaaga atgtatctcc gtcttccgca gttttaaaag ctatcgcagt ggcgaactgg
     7861 tacgcaaaga ggaaaaggaa tgtatgggaa acaccctcta tatcggttca ttacaaagtg
     7921 aagtttattt ctgtatctat gaaaaggact acgagcagta caagaaaaat gatattccca
     7981 ttgaagacgc agaagtaaaa aaccgttttg agattcgatt gaaaaatgag cgtgcctatt
     8041 atgcagtccg tgatttactc gtctatgaca atccagagca taccgccttt aaaattatca
     8101 atcggtatat ccgttttgta gataaagacg attccaaacc tcgttctgat tggaaactga
     8161 atgaagaatg ggcttggttt attgggaaca atcgtgaacg attaaaacta accacaaaac
     8221 cagagcctta ctccttccaa aggacgctga actggctatc tcatcaagtt gccccgacct
     8281 taaaggttgc gattaaactt gatgaaatca accagacgca ggttgtaaaa gacattctcg
     8341 accatgcgaa actgacagac cgacacaagc agattttgaa gcaacagtca gtaaaagaac
     8401 aggacgtgat aacaacaaaa aaataactca aatacaaatt cattgaatat agagaggaga
     8461 acatttttat gaattttgga caaaaccttt ataactggtt tctatcaaac gctcaatcac
     8521 tggtgctttt agcaatcgtt gtgattggct tgtatcttgg cttcaagcgt gagtttagca
     8581 aactgattgg ctttttaatt attgcgatta ttgcggttgg cttagtcttc aacgctgctg
     8641 gagtaaaaga cattttacta gagctattca atcgcattat tggtgcttaa ataaaaccgt
     8701 tcttttgtgg aatataagtg gttttcttat gttccgcaaa ggaatggtac accaaacgaa
     8761 gtgcggtagg gatttttgaa tctctacaaa gaaaggacgt gaatatatgg acgatatgca
     8821 agtctatatt gcgaatttag gcaaatacaa tgagggcgaa ttggtcggtg cgtggtttac
     8881 ctttcccatt gactttgagg aagtcaaaga gaaaatcggc ttgaatgatg aatatgagga
     8941 atacgccatt catgactacg agttaccctt tacggttgac gaatacactt ccattggcga
     9001 actcaatcga ctatgggaaa tggtatcgga attacccgaa gaattacaat cggagctatc
     9061 tgctctgctc actcattttt caagcattga agaactaagc gaacatcaag aggatattat
     9121 cattcattcc gattgtgatg atatgtatga cgtggcacgc tactacattg aagaaacggg
     9181 tgctttaggc gaagtaccag ctagtcttca aaactatatt gattatcaag cctatggtcg
     9241 ggatttagac ctttcaggaa cgtttatctc aaccaatcat gggatttttg aaatcgtcta
     9301 ttaaatctgt cggtacatta ctactggcag attttctatt ttacggggtg gctcaatcag
     9361 ctacccctat tttttatgaa aggattgatt acatgaagaa aatacgaagc tataccagta
     9421 tctggtctgt ggaaaaggta ctgtattcta tcaatgattt tagacttccg tttcccataa
     9481 cctttacgca aatgacatgg tttgtcgtgt cactctttgc agtgatgata cttggcaact
     9541 tgccccctct ttccatgata gagggagcat ttctcaaata ctttgggatt cctgtggctt
     9601 tcacatggtt tatgtctaca aaaacttttg atggtaaaaa gccttatgga tttttgaagt
     9661 ctgtcattgc ttatgcactg cgaccaaagc tgacctatgc aggaaaaaaa gtaacgcttg
     9721 gcagaaacca gccacaagaa gccattacag cagttaggag tgaattttat ggcatatcca
     9781 attaaataca ttgaaaacaa tctcgtctgg aataaagacg gggaatgtta tgcttactat
     9841 gagcttgttc cttacaatta ctcatttcta agtccagaac agaaaataca agtgcatgat
     9901 tctttcagac agcttatcgc acaaaatcgt gatggcaaaa ttcatgcttt acaaatcagt
     9961 acagaatcca gcatacgttc tgcacaagag cgttccaaaa atgaagtcac tggcaagctc
    10021 aaagcggttg cctatgacaa aatcgaccaa cagacagacg ctttaatatc catgattggc
    10081 gaaaatcaag tgaactaccg tttctttatc ggctttaagt tgcttctcaa cgatcaggag
    10141 ttttctatga aaagtcttac cgttgaagca aaaaatgctt tgtctgattt tgtctatgat
    10201 gtgaaccata agctgatggg cgattttgtt agtatgagta atgatgaaat cctgcgtttt
    10261 cagaagatgg aaaagctctt agaaaataaa atctctcgtc gtttcaaaat ccgcaggtta
    10321 gataaggacg acttcggcta tctgattgaa cacctttacg gacagacagg cactgcctat
    10381 gaagagtatg agtaccatct atcaaagaaa aagctggata atgaaacgct gattaaatac
    10441 tatgacttga ttaagcctac tcgctgtttg gtggaagaaa aacagcgata tttgaaaatc
    10501 cagcaggaag atgaaaccgt ctatgtagct tactttacca ttaacagcat tgtcggagaa
    10561 ctggacttcc cgtcctctga aatcttctac taccagcaac agcaatttac attcccgatt
    10621 gatacgtcaa tgaatgtgga aattgtagcg aatcgtaaag ccctatctac tgtccgcaat
    10681 aaaaagaaag aactgaaaga cttggataac cacgcttggc aaagtgataa tgaaaccagc
    10741 tccaatgtgg cggaagctct ggaaagtgtg aatgagctgg aaaccaattt agaccaaagc
    10801 aaggaatcta tgtacaagct gtcttatgtg gtaagggtat cagcaaatga tcttgacgaa
    10861 ctcaaacgtc gttgtaatga agtgaaagat ttttatgacg atttaagcgt aaaactggta
    10921 cgaccatttg gggatatgct cggcttacat gaagaatttt tacctgccag caagcgttat
    10981 atgaatgatt atattcaata cgtgacctct gatttcctcg ctggtttagg ttttggtgct
    11041 actcaaatgc tgggggaaaa tgaggggatt tatgttggct acagcttaga tactggacgc
    11101 aatgtctatc tgaaacctgc tcttgccagt caaggggtta agggttcagt aaccaatgcg
    11161 ttagcgtcgg cttttgttgg ttcgctgggt ggtggtaaat cctttgcgaa taaccttatc
    11221 gtctattatg cggtgcttta tggggcacaa gcagtgattg tagacccaaa agcagaacgt
    11281 ggcagatgga aagaaacctt gccagagatt tcccatgaaa tcaatatcgt cactctgact
    11341 tctgatgaga aaaacaaagg cttacttgac ccttatgtga ttatgaaaaa tcccaaagat
    11401 tctgaatcac tggctattga tattctgaca ttccttacgg ggatttcctc tcgtgatggg
    11461 gaacgcttcc caatccttag aaaagccatt cgtgcagtaa ccaatagtga agtacgaggg
    11521 ttgatgaaag tgattgagga attacgggtt gagaatacgc cactaagtac cagtatagcc
    11581 gaccatatcg aaagttttac agactatgac tttgcacatt tattattcag taatggttat
    11641 gtggagcagt ctatcagctt agaaaaacaa ctgaacatta tacaggttgc ggacttggta
    11701 cttcccgaca aggaaacttc ctttgaggaa tataccacta tggagctttt atccgttgct
    11761 atgctgattg tcattagtac ctttgcttta gactttatcc atacagaccg aagcattttc
    11821 aagattgtag atttagacga agcatggagc tttttacagg tagcacaagg aaaaacacta
    11881 tctatgaagc tggttcgggc tggtcgtgct atgaacgctg gggtatattt cgtgacccaa
    11941 aatacagacg acctcttaga tgaaaaactg aaaaataacc tcggcttaaa atttgcattt
    12001 cgttccactg accttaacga gattaaaaag accttagcct tttttggtgt agacccagag
    12061 gacgaaaaca atcagaagcg attgcgtgat ttggaaaacg ggcaatgcct tatcagtgat
    12121 ttatatggtc gtgtcggtgt gatacagttc caccctgtat ttgaagaact gctccatgcc
    12181 tttgatacca gaccacctgt gcgaaaagag gtgtaaatgt gaaaccatca atagtaaaca
    12241 gaataaaatc aaactggacg ctgaaacgtc taggtaaagt ggcaatgaca gtggctttca
    12301 cacttgtgat tgccattttt cttttagcca tgctgggaac ggtggttcaa gctgcgggct
    12361 tggtagatga tacggtcaat gtggcaaatg aatacagccg atacccactt gaaaactatc
    12421 aactggattt ttatgtggat aatagctggg gctggcttcc gtggaactgg tcggacggga
    12481 ttggaaaaca ggtcatgtat ggactatatg ccattaccaa ttttatttgg acaatcagtt
    12541 tgtatgtttc caatgcgaca ggttacttag tacaggaagc ctattcctta gacttcattt
    12601 ccgctacagc agattccatt ggtaagaata tgcagacctt agctggtgtg agtgcaaacg
    12661 gattttcaac agagggtttc tatgttggat tcctcttact cttgattttg gttcttgggg
    12721 tttatgttgc ctatacggga ctgataaaga gagaaaccac aaaggcaatt catgccatta
    12781 tgaattttgt gctggtgttt atcctatcgg cttcctttat tgcctacgct cccgactaca
    12841 ttaaaaaaat caatgacttt tcatcagaca tcagtaatgc cagtttatca cttggcacga
    12901 agattgtcat gccccattcc gatagtcaag gcaaggacag cgtggactta atcagagata
    12961 gcctgttttc catacaggtt cagcaaccgt ggctactgct tcaatacaac agttcagaca
    13021 ttgaaagtat cggtattgac cgtgtggaaa gcctgctctc caccagccca gattccaaca
    13081 atggcgaaga cagagaaaaa attgttgcgg aagaaattga agacagaagc aataccaatc
    13141 taaccattac aaagaccatt aaccgtttag gtacagtctt cttcctattt gtcttcaata
    13201 ttgggatttc catatttgta ttcctattaa caggaatcat gattttctcg caggtacttt
    13261 ttatcatcta tgctatgttt ctgcctgtga gctttatttt aagcatgatt ccatcatttg
    13321 atggtatgtc aaaacgagcc ataacaaagc tctttaatac cattttgaca cgagctggaa
    13381 tcacattgat tattacgaca gcatttagta tttcaaccat gctctatacc ttatcggctg
    13441 gttatccgtt ctttttgatt gcttttctac agattgtgac ctttgcagga atctacttca
    13501 agctgggcga tttaatgagt atgttttctc tacagagtaa cgattctcaa agtgtgggaa
    13561 gtcgtgtgat gagaaaacct cgtatgctta tgcacgctca catgcaccgt ctacagcgga
    13621 aacttggacg ttccatgact actctagggg ctgggtctgc cattgttaca ggtaaaaaag
    13681 gacagtcggg ttcggggagt tctgcaagga cacaagcaga tcactcccga ccagacggaa
    13741 aggaaaaatc aacacttgga aaacgtatcg gtcaaaccat cggtacagta gctgatacca
    13801 aagacagaat ggtagacact gctagtggtt tgaaagaaca ggttaaagat ttgccgacca
    13861 atgcaagata tgcagtatat caaggaaaat ccaaagtaaa agagaatgtc cgtgatttaa
    13921 ccagtagtat ttctcaaacc aaagcggaca gagccagtgg acgcaaggaa cagcaggaac
    13981 aaaggcgaaa aaccattgcg aagcgtcgct ctgaaatgga acaggtcaaa cagaaaaaac
    14041 agcctgcttc ttctgttcat gaaagaccga ctacaagaca agaacaatat catgatgaac
    14101 agacctcaaa acagtctaat attcagactt catataagga atctcaacaa gccaaacaag
    14161 agcgtccagc agttaagtcc gatttttcaa gtccaaaagt ggaacgccaa ggcaataccg
    14221 ttcaagaaaa aaccgttcaa aagccagcaa cttcaaccac tacagcagat agaacttcac
    14281 aacgtccaat cacaaaagaa cgtccgtcta ctgttcaaag agtaccacta caaaatacaa
    14341 gaagtagacc accaatcaaa accgccacca ttaagaaagt cggtaagaaa ccatgaagtt
    14401 gaaaacttta gtgattggtg gttctggatt attcttgatg gtcttctcac tgcttctgtt
    14461 tgttgccatt ttattttcag atgaacagga cagcggaatt tccaatattc attatggagg
    14521 tgtgaatgtt tccgcagaag tgctggctca taagcctatg gtagaaaaat atgccaaaga
    14581 atatggcgtt gaagaatatg tcaacatact tcttgcgatt atacaggtgg aatcgggcgg
    14641 tactgcggaa gatgttatgc agtcctcgga atccctcggt cttccaccta attcattgag
    14701 tacagaagaa tccattaagc aaggtgtgaa gtatttcagt gaattattag ccagtagcga
    14761 aaggctcagt gtagatttag aatcggttat ccagtcctac aattatggtg gtggtttctt
    14821 agggtatgtg gctaatcgtg gaaataaata tacctttgaa ctggctcaaa gtttctcaaa
    14881 agagtattca ggtggcgaaa aagtgtctta ccccaatccc atagccatac ctatcaatgg
    14941 gggctggcga tacaactatg gcaatatgtt ttatgtgcaa ctggtaacgc agtatcttgt
    15001 cacaacagag tttgatgatg atacggtaca agccatcatg gacgaagcac tgaaatatga
    15061 gggctggcga tacgtttacg gtggagcttc cccgactact tcttttgatt gtagcggact
    15121 gacacaatgg acgtatggaa aagctggaat taacttacca cgaaccgcac aacagcaata
    15181 tgatgtgacc cagcatatcc cactatcgga agcacaagct ggcgatttgg ttttctttca
    15241 ttctacctat aacgctggct cttatattac tcatgttggg atataccttg gcaataaccg
    15301 tatgtttcat gcaggcgacc caatcggtta tgccgactta acaagcccct actggcaaca
    15361 gcatttagtg ggagcaggac gaatcaaaca atgagaaagg aagatttaat gatgaaattt
    15421 agaaaaaatc agaataaaga aaaacagata ccaaaggaaa agaaacctcg tgtctactat
    15481 aaggtcaatc ctcataaaaa ggttgtgatt gccttgtggg tacttttagg gcttagtttc
    15541 agctttgcga tattcaagca ctttacagct atagatactc atactattca cgaaacaact
    15601 atcatagaaa aggaatacgt tgatactcat catgtagaaa attttgtaga gaactttgcg
    15661 aaagtctact attcatggga gcaatccgat aagtccattg ataatcgaat ggaaagtcta
    15721 aaaggctatc tgacagatga acttcaagct ctcaatgttg atacagtacg caaagatatt
    15781 cctgtatcgt cttctgtaag aggatttcag atatggacgg tagagccaac tggcgacaat
    15841 gagtttaatg taacctacag tgtagaccag ctcattacag agggagaaaa tacaaagacc
    15901 gtccactctg cttatatagt gagtgtctat gtagatggtt ctggaaatat ggtactggtt
    15961 aagaatccga ccattaccaa catacctaag aaatcaagtt ataaaccaaa agccattgaa
    16021 agtgagggga cggttgattc cattacaacc aatgaaatca atgagttttt aacgacgttc
    16081 ttcaagctct atcctacagc gacagccagt gaactttcct actatgtgaa tgacgggata
    16141 ttaaaaccaa tcggaaaaga gtacatcttt caagaactgg taaatcctat tcacaatcgt
    16201 aaggataatc aagtcacggt atcgctgaca gtggagtata tcgaccagca gaccaaagca
    16261 acgcaggtat ctcaatttga tttggtactt gaaaagaacg ggagtaattg gaagattata
    16321 gaataacaaa tattggtaca ttattacagc tattttgtaa tcacgtactc tctttgataa
    16381 aaaattggag attcctttac aaatatgctc ttacgtgcta ttatttaagt atctatttaa
    16441 aaggagttaa taaatatgcg gcaaggtatt cttaaataaa ctgtcaattt gatagtggga
    16501 acaaataatt ggatgtcctt ttttaggagg gcttagtttt ttgtacccag tttaaatacc
    16561 tttatcatgt gattctaaag tatccggaga atatctgtat gctttgtatg cctatggtta
    16621 tgcataaaaa tcccagtgat aagagtattt atcactggga tttttatgcc cttttgggct
    16681 tttgaatgga ggaaaatcac atgaaaatta ttaatattgg agttttagct catgttgatg
    16741 cgggaaaaac taccttaaca gaaagcttat tatataacag tggagcgatt acagaattag
    16801 gaagcgtgga caaaggtaca acgaggacgg ataatacgct tttagaacgt cagagaggaa
    16861 ttacaattca gacaggaata acctcttttc agtgggaaaa tacgaaggtg aacatcatag
    16921 acacgccagg acatatggat ttcttagcag aagtatatcg ttcattatca gttttagatg
    16981 gggcaattct actgatttct gcaaaagatg gcgtacaagc acaaactcgt atattatttc
    17041 atgcacttag gaaaatgggg attcccacaa tcttttttat caataagatt gaccaaaatg
    17101 gaattgattt atcaacggtt tatcaggata ttaaagagaa actttctgcc gaaattgtaa
    17161 tcaaacagaa ggtagaactg tatcctaata tgtgtgtgac gaactttacc gaatctgaac
    17221 aatgggatac ggtaatagag ggaaacgatg accttttaga gaaatatatg tccggtaaat
    17281 cattagaagc attggaactc gaacaagagg aaagcataag atttcagaat tgttctctgt
    17341 tccctcttta tcatggaagt gcaaaaagta atatagggat tgataacctt atagaagtta
    17401 ttactaataa attttattca tcaacacatc gaggtccgtc tgaactttgc ggaaatgttt
    17461 tcaaaattga atatacaaaa aaaagacaac gtcttgcata tatacgcctt tatagtggag
    17521 tactacattt acgagattcg gttagagtat cagaaaaaga aaaaataaaa gttacagaaa
    17581 tgtatacttc aataaatggt gaattatgta agattgatag agcttattct ggagaaattg
    17641 ttattttgca aaatgagttt ttgaagttaa atagtgttct tggagataca aaactattgc
    17701 cacagagaaa aaagattgaa aatccgcacc ctctactaca aacaactgtt gaaccgagta
    17761 aacctgaaca gagagaaatg ttgcttgatg cccttttgga aatctcagat agtgatccgc
    17821 ttctacgata ttacgtggat tctacgacac atgaaattat actttctttc ttagggaaag
    17881 tacaaatgga agtgattagt gcactgttgc aagaaaagta tcatgtggag atagaactaa
    17941 aagagcctac agtcatttat atggagagac cgttaaaaaa tgcagaatat accattcaca
    18001 tcgaagtgcc gccaaatcct ttctgggctt ccattggttt atctgtatca ccgcttccgt
    18061 tgggaagtgg aatgcagtat gagagctcgg tttctcttgg atacttaaat caatcatttc
    18121 aaaatgcagt tatggaaggg atacgctatg gttgcgaaca aggattatat ggttggaatg
    18181 tgacggattg taaaatctgt tttaagtacg gtttatacta tagccctgtt agtaccccag
    18241 cagattttcg gatgcttgct cctattgtat tggaacaagt cttaaaaaaa gctggaacag
    18301 aattgttaga gccatatctt agttttaaaa tttatgcgcc acaggaatat ctttcacgag
    18361 catacaacga tgctcctaaa tattgtgcga acatcgtaga cactcaattg aaaaataatg
    18421 aggtcattct tagtggagaa atccctgctc ggtgtattca agaatatcgt agtgatttaa
    18481 ctttctttac aaatggacgt agtgtttgtt taacagagtt aaaagggtac catgttacta
    18541 ccggtgaacc tgtttgccag ccccgtcgtc caaatagtcg gatagataaa gtacgatata
    18601 tgttcaataa aataacttag tgtattttat gttgttatat aaatatggtt tcttgttaaa
    18661 taagatgaaa tattttttaa taaagatttg aattaaagtg taaaggagga gatagttatt
    18721 ataaactaca agtggatatt gtgtcctgta tgtggaaata aaacacgatt aaagataagg
    18781 gaagatactg aattaaaaaa attccccctc tattgtccga aatgcagaca agaaaattta
    18841 attgaaataa agcagttcaa agtaactgtg attacagagc cagacgcaaa gacgcagagc
    18901 cgataaaatg agattaatac aatctcattt tatcggctct ttccgttatg tatggattct
    18961 tttaattagt cttcgatgtt tcttgcttcg ttgataccgc tggctaaaga ttccattaag
    19021 gatagttctt tgtctgtaaa gctatccatg tatttctcta tctgtaatcg tcgggtgctt
    19081 tttaccaagt tattagcagg taagaaaaat tcatcaacgg aaacatgaag taacgataca
    19141 aggtcataaa gaacttgtat gctggggtgt tgccctttat tttcaatatt agttaagtac
    19201 cgtgggtcaa tttcaatcaa tgctcccact tgttcacgag ttaaacctcg tttcaatcga
    19261 gcttctttaa tggctaaacc aaaggctcta aaatcatatt tatcttcttt tttacgcata
    19321 gtagaccacc tctatacatt ttattgttcc tactgaatta aaaacaggta tagaaaaacg
    19381 tgttatatgg tttataggtt tatatttaat aaaaagcact actaaacgcc aataaaaaaa
    19441 accgttatat ggtagtgcta tttacgctgt taaaatattg tatattactt ccaaatggcg
    19501 gtttgttgga ggtcaacgtc gccatgaagt acatcatata caataaattt ccttacattg
    19561 ggttcttgtc aaaaaaagtc gtctatctgc aatagataag tacgtccacc aatgtggttt
    19621 tataaatcat atagatagaa taacagaagc atgtaaacag agaaataaat ctgtttatat
    19681 gcttttttgg ctattcagaa cttttttaca aagtttattt atcagtaatg caacaaatcc
    19741 ccctttcaca ttgggactaa gagtgaaagg agataaacga gcaaggctca cttcctttcc
    19801 tagacagaaa gggggtgaga aacatgaaac catcttcttt tcagaccaca atagaaaatc
    19861 agtttgacta tatctgtaaa cgtgctatgg aagacgagcg aaagaattat atgctttatc
    19921 tttcaaggat tgcaaagcgt gaggtgtcct tttcggatgt tggcgattat cttgttagcc
    19981 agtttgcgac aacagataac tattcaactg actttcagat ttttacactc aatgggttat
    20041 cagtaggcgt tgaaaatgat ttgttgagtg aagcattacg tgagttgcca gacaagaaac
    20101 gtgaaattct actgctgttt tactttatgg acatgagcga ttcagaaatt gcagacctgt
    20161 tgaaattgaa ccgttctact gtctatcggc atagaaccag tggactagcc ttaattaaaa
    20221 agtttatgga ggaatttgaa gaatgaaaac acaatatcct atgattccct ttcctctcat
    20281 tgtaaaggca acagatggcg ataccgaagc gattaaccag attctacatc attacagagg
    20341 gtacataacg aagcgttccc tacgacttat gaaagatgaa tatggcaatc aaagtatggt
    20401 cgttgatgaa gtcttacgtg gaagaatgga aaccagactg attacaaaga ttttgtcatt
    20461 tgaaattaag taatatcctc tctcctttcg tggaagcgtg ctaaaccatt ccacgcttcc
    20521 cgaacaggga ggtttgttat tccaccaaag catattgagc tttcaatgtg ttttgatagg
    20581 ctaacgagcc attgttcttt gaaaactgaa taaaagtaat cgaatacgtt tcgataagaa
    20641 aagagccaac ggaactaacc gccatgacct atcttataaa gatagcgagc gattcatgtt
    20701 agtgatccga gaagcaatct ttagcaggat tgcctgcaac gacattctta tcgtgataat
    20761 gatactccca tacagtcaat agtccgagcg tgataaaacc gtcgcaggca atgagtatgg
    20821 ctacatgaga accatgcagg ggtggaactc ccgtgagctt tgctaaagct gttcgattgc
    20881 tggtaaaaca acttttatga aatccaaata agtgatttgg aaaggaggat tttatgaagc
    20941 agactgacat tcctatttgg gaacgttata ccctaaccat tgaagaagcg tcaaaatatt
    21001 ttcgtattgg cgaaaacaag ctacgacgct tggcagagga aaataaaaat gcaaattggc
    21061 tgattatgaa tggcaatcgt attcagatta aacgaaaaca atttgaaaaa attatagata
    21121 cattggacgc aatctagcgt cgccaaaggg tcttgtatat gataaaatag tattaagtcg
    21181 tatcaaggct ctttccataa aggaaaggag caaatgccat gtcagaaaaa agacgtgaca
    21241 ataaaggtcg aatcttaaag actggagaga gccaacgaaa agacggaaga tacttataca
    21301 aatatataga ttcatttgga gaaccgcaat ttgtttactc gtggaaactt gtggctacag
    21361 accgagtacc agcaggaaag cgtgattgta tctcacttag agagaaaatc gcagagttac
    21421 agaaagacat tcatgatggt attgatgttg taggaaagaa aatgacactc tgccagcttt
    21481 acgcaaaaca gaacgctcaa agaccaaagg ttagaaaaaa cactgaaact ggacgcaaat
    21541 atcttatgga tattttgaag aaagacaagt taggtgtaag aagtattgac agtattaagc
    21601 catcagacgc taaagaatgg gctattagaa tgagtgaaaa tggttatgct tatcaaacca
    21661 tcaataacta caaacgttct ttaaaggctt cattctatat tgctatacaa gatgattgtg
    21721 ttcggaagaa tccatttgac tttcaactga aagcagttct tgatgatgat actgtcccta
    21781 agaccgtact aacagaagaa caggaagaaa aactgttagc ctttgcaaaa gctgataaaa
    21841 cctacagcaa aaattatgat gaaattctga tactcttaaa aacaggtctt cgtatttcag
    21901 agtttggtgg tttgacactt ccagatttag attttgagaa tcgtcttgtc aatatagacc
    21961 atcagctatt gagagatact gaaattgggt actacattga aacaccaaag accaaaagtg
    22021 gcgaacgtca agttcctatg gttgaagaag cctatcaagc atttaagcga gtgttagcga
    22081 atcgaaagaa tgataagcgt gttgagattg atggatatag tgatttcctc tttcttaata
    22141 gaaagaacta tccaaaagtg gcaagtgatt acaacggcat gatgaaaggt cttgttaaga
    22201 aatacaataa gtataacgag gataaattgc cacacatcac tccacatagt ttgcgacata
    22261 cattctgtac caactatgca aatgcaggaa tgaatccaaa ggcattacag tacattatgg
    22321 gacatgctaa tatagccatg acgctgaact attacgcaca tgcaacattc gattctgcaa
    22381 tggcagaaat gaaacgcttg aataaagaga agcaacagga gcgtcttgtt gcttagtagt
    22441 acaaatgaat ttactactta tttaccactt ctgacagcta agacatgagg aaatatgcaa
    22501 agaaacgtga agtatcttcc tacagtaaaa atactcgaaa gcacatagaa taaggcttta
    22561 cgagcattta agaaaatata aaaagataat tagaaattta tactttgttt aatttaaaaa
    22621 atatccagat aaaaacttta gtaatttaga ttattgctta ataggaagta aatgttcaaa
    22681 tagttttata aaagaaaagt tgattacttt ttttaaagtg agaataccag atatattaaa
    22741 agacaaaagt atattaaaag cagagttatt tattcatatt gattcaaata agaatcatat
    22801 ttttaaagaa aaagtagata ttgaaattaa aagaataagt gaatattata atttacgaac
    22861 tataacatgg aatgatagag tgtctatgga aaatatcagg gggtatttac caattggtat
    22921 aagtgataca tccaactata tttgtttaaa tattacagga actataaaag catgggcaat
    22981 gaataaatat cctaattatg ggttagcttt atctttaaat tacccttatc agattcttga
    23041 atttacatct agtagaggtt gtaacaaacc gtatatactt gtaacatttg aagatagaat
    23101 tatagataat tgttatccta aatgtgagtg tcctccaatt agaattacag gtccaatggg
    23161 accaagagga gcgacaggac caagaggagc aacaggtccg acaggaagta caggaccaac
    23221 gggagaaata ggcccaacag gaaatacggg agcaacagga gcgacaggaa gtataggacc
    23281 aacgggagta aca
//
LOCUS       DAEPXF010000424        22894 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain HB27
            SAMN13376389-rid12479423.guided.054, whole genome shotgun sequence.
ACCESSION   DAEPXF010000424 DAEPXF010000000
VERSION     DAEPXF010000424.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13376389
            Sequence Read Archive: SRR10518432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 22894)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22894)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001069624.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001069624.1
            Genome Coverage       :: 315x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/15/2021 01:54:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,165
            CDSs (total)                      :: 4,076
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 89
            rRNAs                             :: 10, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 10 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 19 of 69
            Pseudo Genes (incomplete)         :: 44 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 10 of 69
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22894
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13376389-rid12479423.guided.054"
                     /strain="HB27"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="China: Hebei"
                     /collection_date="2017"
     gene            82..798
                     /locus_tag="KRL67_004102"
     CDS             82..798
                     /locus_tag="KRL67_004102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH1524867.1"
                     /translation="MILIVILSIFIIASINIIILGKENIRDIVIPLTLFIVSDFLFLC
                     GNIICRGGVMLLFIGLFAIPLMLIAFIWICVHMIKLYYKKGLNKRKMIGCIFSIFISI
                     GTIFIPSPSESFRFKLYEKDYSVVAKAILKVYDEKKLSSECVFYIQSDKNELEKIFSK
                     EVIKKMKKLNRNLDVVKISGDRNLVYFFFGAELQSIDGIVIFKDLKHVKTKLDFINRS
                     IQAPRLEYITNNVYYFKGEL"
     gene            826..1764
                     /gene="blaCDD"
                     /locus_tag="KRL67_004103"
     CDS             826..1764
                     /gene="blaCDD"
                     /locus_tag="KRL67_004103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDD family class D beta-lactamase"
                     /protein_id="HBH1524868.1"
                     /translation="MKRKNNFIWIVILLVVVAMGIYYVGRKHNNTKQKYDKNYINSEL
                     NKSKIENNDKNKKEKINIVDYSDCFEGISGGAIFYNTKNKEYNIYNKELIETRRSPCS
                     TFKIVSTLIGLEKGVINSKESVMGYDGTNYPNKNWNKNLSLEEAFKESCVWYYKKLIN
                     KVDAKSVQNILDDLKYGNCDISEWEGDLKNGKGHLNGFWLESSLQISPKEQVQTMAKI
                     FEGDTNFKKEHINILKDIMKIDVNDKNINVYGKTGTGFDEKNKRVDAWFVGMLEREGD
                     TYYFAIKSDDSNKEITGPKVKEIAINIIKKYYSVRE"
     gene            2108..2791
                     /locus_tag="KRL67_004104"
     CDS             2108..2791
                     /locus_tag="KRL67_004104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBH1524869.1"
                     /translation="MEILKCENLTKIYGSNQTRVTALNNVNLSVQKGDFVSIVGASGS
                     GKSTLLHMLGGVDRPTSGKIYVEDTEISSLKEEALAVFRRRKVGLIYQFYNLIPTLDV
                     RKNILLPMLLDKRKVDEDRFSEIVSILGLSDRLNHLPSQLSGGQQQRVSIARSLIYRP
                     AILLADEPTGNLDRKNSEEIVDLLNLSNKRFNQTILLITHDEKIALEANRIVTMEDGV
                     IVSEKVVKK"
     gene            2788..5448
                     /locus_tag="KRL67_004105"
     CDS             2788..5448
                     /locus_tag="KRL67_004105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HBH1524870.1"
                     /translation="MNILQEYTLDFVRRNKRSSIAIMLAILLTTTMMSSLCGLLYTMW
                     TDFIRLSIEKEGNWHGELFDDTYGKDLSLIENFSSVDDVMVKGQWYVGKINNDKRDYI
                     IYRNATSEYWNHMPEKDTIIEGRRPSNTNEIALSKQYFENNPSVKIGDKITLPIGDRI
                     LNGKPLEPIAPKSKGESFKKISETTLTIVGKVDVSTSSVIPAYTALGYLDKTSIKPND
                     SITVYLRFKNIRDTYKELPKLAKSLGWKTNEYGKYNLKYNSNYLLKMLVFSPEQKASM
                     SSISKFSTPIMYLTIAIFTVAVFVMVIYNAFSLSANARLTQLGILSSVGASPKQIKRS
                     VVFEGFLLTIIPLPIGLFLGWLLCNRLIVYINSVNYHTDAPEVVFTYGIPAFLPAVLL
                     TIVTVWISALIPARKVSKISPIEAIRQGDSVKIKKSHKYSLGKIFGIEGELASNALRA
                     RKKSYRTATISLTLSFLLLTGFLHIIANQEGAKAVYGSDNYKDQRDIAVYLENSEPVE
                     STFIEKLDNIEGVKSSLYYQKMSAATWLTEKDASKELAKNGGFKSIVSSKKYSPIERD
                     GKFRIISNIIGLDDKSFGEYCRQISVDPKLFYDTKNPTSIVYNKEEDVNRSTRRNKVY
                     IDYLSISIGDNIKLNEKVYDEDKGDYTYNMKVGAITSTLPKIVDTSSRYTLMQIVPMS
                     MVQEISKNYDEVSRLRSNRLIGLFKVNNRKDISRVREKLESICNKDYGSGDYSIEDVL
                     ESEKQDASGRKTMNLIVGFITGLLAMIGLSNVLATVSGNIRSRRQEFAMLRSVGLSPD
                     GIKKMLVLEGLFLGITPLLLSIPVQIGIVYTFLRINEIYFIEYLPFAPVSTIIGFTIL
                     ILFIVIASYMTGYKQLKNENIVESIKNETI"
     gene            complement(5629..6810)
                     /locus_tag="KRL67_004106"
     CDS             complement(5629..6810)
                     /locus_tag="KRL67_004106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBH1524871.1"
                     /translation="MKTPRNINSTVVFIIFCLIVTFLLSVILTTITSFNEYNVLSKAL
                     GASYEYNPKSTESILKSLKNSSDEDFKTGNEILSKHGYSKSTFWSKNFFYFLVVSFGI
                     ISTMTIVLYIIKHRTNKQKLSRINNLTQYLEEVNLGKESVLLRCEDEFSHLEDEIYKT
                     VGELRCSKEFALKERQTLSDNLADIAHQLKTPITSMSLMAQLLSENCPDEEIDYINRL
                     TNQISRLERLVSSLLTLSKLDTSTLIFESKLIDVHSLLTYAIEPIEGSLREKHQTFTI
                     EGSPSVEFEIDINWTLEALLNILKNCSEHIEDGGYIVAYYSQNPLYVEIIIEDNGNGF
                     AQEELPYIFNRFYRGKNASKDSIGIGLALSKSIIERQNGTLHAENCHNGGARFIIKFY
                     T"
     gene            complement(6810..7472)
                     /locus_tag="KRL67_004107"
     CDS             complement(6810..7472)
                     /locus_tag="KRL67_004107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBH1524872.1"
                     /translation="MAKILLLEDDDTITFGIKTSLEKKNHTVCCYNTITEAKKNFDKT
                     IDLILLDLNLPDGIGYEFCQYVKSIEDTPVIFLTVRDDEKDIIRGLDMGADDYVVKPF
                     LLSVLQSRINAVLRRTHSNTNNTITCGNISLNKVEVKAYLDNVEVPLTAGEYKLLLSL
                     MENKNRAMTRTKLLEMLWDVDGNFVNDNTLTVTIKRLREKLKNTPCIKTLRGIGYRME
                     DS"
     gene            complement(7617..8255)
                     /locus_tag="KRL67_004108"
     CDS             complement(7617..8255)
                     /locus_tag="KRL67_004108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBH1524873.1"
                     /translation="MSELSKRDKEKIQRENEIIDKAEKLFCLNGFDSTTMSELAKEVE
                     YTKRTIYKYFSCKEDLFFAVVLRGYKRLWDNVKIESAKGKTGFEKIKLSYFAFHKFYS
                     VEPSLLCLMGMIGIVNSEKSDTDMLFKEKFFSFNKFMFDEIQEMFEIGKNDRSIRHDI
                     EIPTLMYSSIFTLTGFFNLLSVTGKSYLNNFNIDEERFIETTLALLIDSIKA"
     gene            8434..9039
                     /locus_tag="KRL67_004109"
     CDS             8434..9039
                     /locus_tag="KRL67_004109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HBH1524874.1"
                     /translation="MKIKENVLMLDSTRGSNCFIVFDKEIILIDTGLPFMGKNIIKEL
                     KTLGIKLTDIKHILLTHHDVDHISNIKLLKEKTGAKVWAHVEDIPFIIGEKDRPGFKK
                     FIGKAISRRLIKDVEPYGENMQIGNIKIIHTPGHTPGHVCMIFEDVLFAGDLVKNKNG
                     NIVPYPNPWNWDYKKMMKSIRELDNLKYEWICMSHGTPCIK"
     gene            9140..9862
                     /locus_tag="KRL67_004110"
     CDS             9140..9862
                     /locus_tag="KRL67_004110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBH1524875.1"
                     /translation="MIRTLILYYLNIKPTHGYEIQKFLQVSGADRWTKIQSGSIYYAL
                     AKLEKDGGVRVLREEKTGARIRKIYEITQSGKLELREEIQKELQMPIVPTGSNKFLLH
                     NILDVLPKDTLQKNLEKHIKYLIEQKKYWENWKEIKKIDKKSLATEKIAFDMTIDNLN
                     YQILWHEEILNNIDKYISVGCETQNIIKSIDFSNIEEDFLFKSDTTNELLEVQRLRDE
                     IINNPDKAIENIDKIILKLQNK"
     gene            9967..10725
                     /locus_tag="KRL67_004111"
     CDS             9967..10725
                     /locus_tag="KRL67_004111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBH1524876.1"
                     /translation="MSNVLLEIKNLSKKYNNKEVVSRESFKLLEGDILGFIGPNGAGK
                     STSINMLTTIVSPDSGSIYYKGKDIKNQQNIFKSSLGVVPQELAIYEDLSAYDNVKFF
                     CSLYGFKGNELKRRTKDALEFVGLWERNKEFPLKFSGGMKRRLNIACAIAHSPKILIM
                     DEPTVGIDPQSRNKILKAIKDINANGTTVIYTSHYMEEIETLCNRIVLIDKGVIIEDL
                     DKSLYKNKYLEFGCKTLEDIFLYLTGTDLRDLEE"
     gene            10730..11470
                     /locus_tag="KRL67_004112"
     CDS             10730..11470
                     /locus_tag="KRL67_004112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBH1524877.1"
                     /translation="MRYFLPLFKLGIKRRSKDFFILFYNIVFPVIIIMLLGYLTSKSY
                     GEEFTSYNYYTIVTIPFCVLMGIISVSYVAQDEKISNTSYRYMISPISIVDLVISKLF
                     SCTAVLSICNLITLAVLRGIFHMSFGGKFLVVIFFLMCESIAVVSMGLFLGLVCKNLD
                     MLRNIINIPIVIFGFLGGVFFPVSSLNPVISLIINISPLTWINRGIVACIYDNNLQLL
                     FNISCIFVAVSIFITILTIKFFKKEVFI"
     gene            11470..12522
                     /locus_tag="KRL67_004113"
     CDS             11470..12522
                     /locus_tag="KRL67_004113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBH1524878.1"
                     /translation="MNVINVFNSNLKRNINKRLVFLVTLLFPIIIVILGVLANYISKP
                     SFNIGILDEYKNERVLKESEKRTYENTIMALENTQGINTEIADFKTIKTDIITGKYSA
                     VICFNKNGFKLYSIKDKNTNDTLKYLIETYKKDPVPVDISKLQENTLGVLQRIIAFIV
                     LFLMITSTVTASMIIKDKNSGTFTRVLYSPQNIRGYVLGNMLYNFTITYFQYIVSISI
                     IKLFNIDIGMDYIDLILMGVWISALATSFGTFVSSLFNKEMHANLFSTCISLILSLIG
                     GSFISVEKMPTMLQKISIISPTQWFISFVTYIEERDGSFFNMNYICILTSIIIILALL
                     AMKFIQRSKYSSNRNY"
     gene            12804..13553
                     /locus_tag="KRL67_004114"
     CDS             12804..13553
                     /locus_tag="KRL67_004114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HBH1524879.1"
                     /translation="MRLESLVNQNYDNLNENDKIIIKQILICQREYRNFSCEKIANEC
                     NVSRATLLRLCRKIGLDSFSDLKYLLKNNQLEFVNNDNNFHDVCENYRLLMNELQKIS
                     FQDICKIIYDAETIYIYGTGNEQKSLAQEIKRIFLSVGKCVIDLFDIGEIEFMRESFQ
                     KSDLFIIISLSGETKAGIEIAKFINNHIHTISLTRLDNNTIATLCEYNLYVETQKLDT
                     LQAISYEVVGAFYALLDLLHMNYIEYRRRKE"
     gene            13550..14314
                     /locus_tag="KRL67_004115"
     CDS             13550..14314
                     /locus_tag="KRL67_004115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HBH1524880.1"
                     /translation="MKIEGLFNKYYNSFTANDKYICECILNHKEDCIKLSIDEFADKY
                     HISTSALSRFAQKLQLPGYSELRVIIRLNETEVEYKNQSKHQLMDCYNKVIDDIEKKD
                     CSIMFERMKKANRIIVFGEGYSQERVAKEMKRIFLPTGKIIYDVYGYDMIQALNHFIK
                     TDDMIFFISLNGDASTIIDFAKKMKMKGIYMVSITKMISNPLARLCDENLYIQSISIS
                     VDKQLDYDVTTPYFILIELLYIKYKMYLESEHIPRK"
     gene            14413..15234
                     /locus_tag="KRL67_004116"
     CDS             14413..15234
                     /locus_tag="KRL67_004116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HBH1524881.1"
                     /translation="MKLYKLLILDIDGTLRDEVDGIPESAKHAIRLCQKNHCSVVICT
                     GRSMGTIQDDVLSLGVDGYIAGGGNYIQYHGELLYNQSFNQRLIKKVVCLLKKREVAF
                     SIESREKVFMNQKAKEIFETMNQLKGTNSCINKQHIQEKITYENNIEEYKSQDIHKIC
                     LWSNEKVFDEVKDILQDKMELAQRDISSQYYEIIQKDFHKGKAIQRLQERLGITQKET
                     ICFGDGQNDIVMFQASDITIAMKNSHQQLKDIATSICEDIFDNGIYKELKRRNII"
     gene            15248..16912
                     /locus_tag="KRL67_004117"
     CDS             15248..16912
                     /locus_tag="KRL67_004117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HBH1524882.1"
                     /translation="MEKKWWQKEIVYQIYPRSFQDSNNDGIGDLQGIVSRLDYLKDLG
                     ITSLWLCPIFKSPMDDNGYDISDYLDIASEFGSMEDLKELIKESKDRGIKIILDLVLN
                     HTSDEHPWFQEALKNPESPYHDYYIFKKGNDLPNNWRSVFGGSVWEKVENRDEYYFHS
                     FGKKQPDLNWENPILRSELYQMINTWSQMGVAGFRVDAITFIKKNLSFQSLESDGVDG
                     LVKCTKAVRNQPGIEKMLYELKENTFKKYDCMTVAEAAGISYDQLGEFIGEDGFFSMV
                     FDFKHADLDVASGSEWFKRISWTISDLRKYILDSQMYVQQHGWAANFIENHDQPRATT
                     KYLLQYERNDDAVKMLGAMYFFLRGTPFIYQGQELGMTNFKRNTINDFNDLSSIDQYY
                     RSIDEGYSEEEALEFVNLRSRDNARTPFPWSDKENAGFSDTTPWLKVIENYKDINAQS
                     QINNSESILEFYKKMIKLRQDSEYSDCLIYGSIEPIDIESDNVIAYRRQLDLVVIDCY
                     YNFSDKPVEIKTEEKYDIVFQNLDDINLLNKTVSLKPFQAVLLKVR"
     gene            16917..18791
                     /locus_tag="KRL67_004118"
     CDS             16917..18791
                     /locus_tag="KRL67_004118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HBH1524883.1"
                     /translation="MNKYNKIANELIEIIGENNIISITHCATRLRVMVKDREIINDKK
                     VEKVNEVKGVFFTSGQYQIILGTGIVNKVYAEVEKMGLKTLSKKEQDELVKNNETGFK
                     KVMRTLADIFVPIIPVIAATGLFLGLKGCLFNDNVLGLFGMSSANIPLYIQTLVSVLT
                     ETAFAFLPAIIVWSAFKVFGGTPVIGLVIGLMLVSPILPNAYSVADPSNEVEAIMAFG
                     FIPIVGCQGSVLTAIVTAFIGANLEKWFRKHMPNVLDLIFTPFFVMLITMLVILLGVG
                     PIMHTIELKMVDIISLLIGLPLGIGGFIIGFTYPLAVITGLHHTYVMIETSLLANTGF
                     NALITLCAMYGFANIGTCLAFMKKSKNNQVKQTAVGAMLSQLFGISEPVLFGIQLRYN
                     LKPLIIMCASSGLGAAILSILHIQSNSYGLAVLPSYLMYIYDGYNLITYLLVSIFVVV
                     FCFIATCLFGVPKEAINEDEDEELVFNENNENFVSPAKGKIVALENVPDETFSKKMLG
                     DGFAIDIIDGKIVSPISGKLETVFSSGHAFGIKGTNGEVLIHVGIDTVELNGDGFDVA
                     VKQGDMVKQGDVLVNVDLKRIHELGKSTLTMVLFPDGKKVNILDINKDVKIGQRICVE
                     "
     gene            complement(19003..19662)
                     /locus_tag="KRL67_004119"
     CDS             complement(19003..19662)
                     /locus_tag="KRL67_004119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chloramphenicol acetyltransferase CAT"
                     /protein_id="HBH1524884.1"
                     /translation="MNKQKFHKIDLQNWERYTHFYYFSKMTNVGFNLTIEIDITNLLK
                     ETKKRNIKFYPAYLYTITTCLNRYRELKIAYDEDELGYWNTLTPLYAIFHDDNKTFSS
                     IWTEYDENFSIFYENYLADVKEYGKNHGFLAKPNPPRNCYTVSCLPWISFKSFTVHNH
                     GLENYFFPTIEAGKYYQKDEKILLPLSITVHHATTDGYHVSCFIDYIQKMCFNTKIWL
                     H"
     gene            complement(19655..20233)
                     /locus_tag="KRL67_004120"
     CDS             complement(19655..20233)
                     /locus_tag="KRL67_004120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBH1524885.1"
                     /translation="MPTKGEQTKQFIIDTSIMLFSEKGYSNVTMKDICEACNLSRGGL
                     YRHFNSPKEIFISILNTHKYYSQNLLDKGIKENISAKHMLEYFFQQQKADINGKYCGF
                     DFAIHEFAFVEKDQRDYMNKRYNSAITMLSNLIDYGQSKSEFKKCDSKSVAIHIMFLF
                     DSMKTSASILKLTNKDIDNQINIIKDMVILYE"
     gene            20594..22681
                     /locus_tag="KRL67_004121"
     CDS             20594..22681
                     /locus_tag="KRL67_004121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HBH1524886.1"
                     /translation="MIKVVYMRKNKKTKKMIIFGFLMCLLIVIIVFLAKFILDGLNKN
                     KPDEVFKQYMSFANKKQYEKMYDLLDEKSKSENKKEDFVLRNKKIYEGIDAHDISIKI
                     NDIKKNKNNDKVINYDSKMDTLAGEISFSNEVLLTRDRQKNYKIKWQSNVIFPELEED
                     NTVRVSKLKGKRGRILDRNGVMIAGQGLASMIGLVPGKMSDNIEDLKKLSTLLNVSVE
                     QIEKKLNASWVKENSMVPIKTIEKIKENADGTVKEEDKELQESLLSIPGVKISNTEVR
                     VYPFGEKTGHLTGYVQNVNAEILKEKEGKRYNDNSIIGKIGLENLLEDRIRGIDGYEI
                     IIADRYGDKKETLVTEPKVDGEDVKLTIDSKLQSKLYDQMKNDKGCAVVMNPKTGEVL
                     SLVSSPSYNPNEFILGMSEDRWNELNKNENKPMYNRFKARLCPGSSFKPVTAGIGITT
                     GKINPNENFGHSSLSWQKDSSWGSYKVTTLKDYGNTANMKNALIYSDNIYFAKAALKI
                     GEDVLAKELLKLGFDESMPFEFGLSSSKFGTDNKFETEIQLADTGYGQGKLLVNPVHT
                     ASIYSAFVNDGDMIKPHLEFKKAEFWKKGVFSKEAVKIIRDDLLQVVESPDGTGHSAM
                     IKGISIAGKTGTAEIKASKDDVTGTELGWFNAFTVDENSDKQYLIIAMIEDVKSRGGS
                     HYVIPIVKSVFED"
BASE COUNT         8640 a         2561 c         3810 g         7883 t
ORIGIN      
        1 ctattgtaat atttaaatat tacaatagta agttttaaat aaagaacaaa taattacctc
       61 acaaactcaa aggaggacat aatgatacta atagttatat tgagtatatt catcatagcc
      121 tcaatcaaca taattatatt aggtaaagaa aatataagag atatagttat ccctttaact
      181 ctatttattg taagtgattt tttatttttg tgtggaaata taatatgtag gggaggagta
      241 atgctactat ttataggttt atttgcaata ccattgatgc taattgcatt tatatggatt
      301 tgtgtacata tgattaagct ttactataaa aaaggtttaa ataaaagaaa aatgattgga
      361 tgtatattta gtatctttat aagtataggg actatattta ttcctagtcc aagtgaaagt
      421 tttagattta aactttatga aaaagattat agtgtagtag ctaaagctat tttgaaagtt
      481 tatgatgaaa aaaagttaag ctctgaatgt gtcttttata ttcaatctga taaaaatgaa
      541 ttagagaaga tattttctaa agaagtaata aaaaaaatga aaaaactaaa cagaaattta
      601 gacgtagtta aaatttctgg ggatagaaat ttagtctatt ttttctttgg agctgaatta
      661 caatctattg atggtattgt gatttttaaa gacttaaaac atgttaaaac aaagttggat
      721 tttataaaca gaagtattca agctcctaga ttggagtata ttacaaataa tgtatattat
      781 tttaaaggtg aactataaac ttatgtagag gagatggtat aaagtatgaa aagaaaaaat
      841 aattttattt ggatagttat tcttctagtt gttgtagcta tgggaatata ctatgtagga
      901 agaaagcata acaatacaaa acaaaaatat gataagaatt atataaactc tgaattaaat
      961 aaatctaaaa ttgaaaacaa tgataaaaac aaaaaagaaa aaattaatat agttgactat
     1021 agtgattgtt ttgaaggtat tagtggagga gctatttttt ataatactaa aaataaagaa
     1081 tataatatat ataacaaaga attaattgaa acaagaaggt caccatgttc tacttttaaa
     1141 atagtttcaa ctctaattgg tttagaaaaa ggggtaataa actcaaaaga atccgttatg
     1201 gggtatgatg gtacaaatta tccaaataaa aattggaata aaaatcttag tttagaagag
     1261 gcatttaagg agtcttgtgt ttggtactac aaaaaattga taaataaagt tgatgctaaa
     1321 tctgtccaaa atattttaga tgatttaaag tatgggaatt gcgatattag tgaatgggaa
     1381 ggagatttaa aaaatggaaa gggacattta aatggatttt ggttagaatc ttcattacaa
     1441 atatctccaa aagaacaagt gcaaacaatg gcaaaaatat ttgagggaga tacaaacttt
     1501 aaaaaagagc atattaatat tttaaaagat attatgaaaa tagatgtaaa tgataaaaac
     1561 ataaatgtat atggaaaaac tggaacagga tttgatgaaa aaaataaacg tgtggatgca
     1621 tggtttgttg gtatgctaga gcgtgaaggt gatacctatt attttgctat taaatcagat
     1681 gattccaata aagaaattac agggccaaag gtaaaggaaa ttgcaattaa tataattaag
     1741 aaatattata gtgtaaggga ataaacttaa ttttatttgt aaatattggt tacctatagt
     1801 ctgtaatatt tattaaataa ttttactaaa ttgagtctat atagagcaaa actcctttag
     1861 tacataaaat acttgctatt gattattaaa ttaaatacct atagatttaa agttaatatc
     1921 ttttaaagac tatgtaatat attaaaagta taaataaaat atattgcata gtttttgttt
     1981 tatattataa ttttaaataa tgtgcaaaaa ctaaacaata tttttaatag taatcgctat
     2041 ttatatttat gtcactataa tgtcatttta ggttgctaaa atttaaccat aaaggagatg
     2101 attgtatatg gaaatattaa aatgtgaaaa tctcacaaaa atatatggtt caaatcaaac
     2161 cagagtgaca gcccttaata atgtaaattt atcagttcaa aaaggtgatt ttgtatccat
     2221 tgtaggagca tctggttctg gtaaatctac attgctacat atgcttgggg gagtagatag
     2281 acctacatct ggaaagattt atgttgaaga tacagaaata tcaagtttaa aggaagaagc
     2341 tcttgcagtt tttagacgta gaaaagtagg tcttatttat caattttata atcttatacc
     2401 tactcttgat gttagaaaaa atatattatt acccatgtta cttgataaaa gaaaggtgga
     2461 tgaggataga ttctctgaga ttgtatctat attgggatta tctgatagat taaaccatct
     2521 tccaagtcaa ctttcaggtg gacaacaaca aagggtatct atagctagaa gtttaatcta
     2581 tagaccagct attttattag cagatgagcc aactggtaat ctcgatagaa aaaattcaga
     2641 agaaattgta gatttgttaa acttatcaaa taaaagattt aatcagacaa tacttcttat
     2701 tactcatgat gagaaaatag cactagaggc aaatcgtatt gtaactatgg aagatggagt
     2761 gattgtctct gaaaaggtgg tgaagaaatg aatattcttc aagaatacac cttagatttt
     2821 gtacgacgca ataaacgtag tagtatagct ataatgttgg ctatattact tactacaaca
     2881 atgatgtctt ccttgtgtgg actgttgtat actatgtgga ctgattttat aagactttca
     2941 attgagaaag aaggtaactg gcatggtgaa ttgtttgacg atacttatgg aaaagactta
     3001 tcgttaattg aaaatttttc ttcagttgat gatgtaatgg taaaaggaca atggtatgta
     3061 ggcaaaatta ataatgacaa aagagattat ataatttata gaaatgctac atcagagtat
     3121 tggaatcata tgccagaaaa agacactata atagagggtc gtcgaccaag taatactaat
     3181 gaaattgcac tatcaaaaca atattttgag aataatccaa gtgtaaaaat tggtgataaa
     3241 attactctcc caataggaga tagaatccta aatggaaagc ctcttgaacc catagcccca
     3301 aaatcgaaag gggaaagttt taaaaaaatt tcagagacta cattgacaat agtaggaaag
     3361 gtagatgttt ctacttcatc agtaatacct gcatatacag ctcttggcta tcttgataaa
     3421 acaagtataa aacctaatga tagtataact gtgtatttgc gatttaaaaa tataagagat
     3481 acgtataagg aacttccaaa acttgctaaa tcattggggt ggaagactaa tgaatatgga
     3541 aaatacaatc ttaaatacaa tagtaattat cttttaaaga tgttagtctt ttccccagaa
     3601 cagaaagcat ctatgagtag tatatcaaaa ttttcaacgc ctattatgta tcttacaata
     3661 gcaattttta cagtagccgt gtttgtaatg gtgatttaca atgcattttc actgtctgca
     3721 aatgctagac ttactcaatt agggatatta tctagtgttg gtgcatcacc aaaacaaatt
     3781 aaaaggtctg tagtgtttga aggcttttta ctgacaatta taccattacc aataggttta
     3841 tttttgggat ggctattgtg taataggctt atagtctata ttaattcagt taattatcat
     3901 actgatgctc cagaagtggt atttacttat ggtattcctg cttttctgcc agcagtttta
     3961 cttacaatag taacagtctg gatttctgca cttataccag cacgaaaagt atcaaaaata
     4021 tctcctatag aggcaatacg acaaggagat agtgttaaaa tcaaaaagtc acataaatat
     4081 tcattaggaa agatatttgg aatagaagga gaacttgcat caaatgcact acgtgctcgt
     4141 aaaaaatctt atcgtactgc aacaatatct ttaacattat cttttttatt acttacaggt
     4201 tttttgcata taattgccaa tcaagaagga gcaaaagctg tttatggaag tgataattat
     4261 aaagaccaaa gagatatagc agtttatttg gaaaatagtg aacctgtaga gtctactttt
     4321 attgaaaaat tagataatat agaaggtgta aagagctcac tatattatca aaaaatgtct
     4381 gctgccactt ggctgactga aaaagatgct tcaaaagaac ttgctaaaaa tggaggattt
     4441 aaaagtatag tttcatctaa aaaatactct cctatagaac gtgatggcaa atttcgtata
     4501 atttcaaata taattgggct tgatgataag agttttggag aatattgcag acaaatttct
     4561 gtagacccaa aattatttta tgatacaaaa aatccaacct caattgtata taataaggag
     4621 gaagatgtta atcgtagcac acgtagaaat aaagtgtata tagattattt aagtatttca
     4681 ataggagata acattaaatt aaatgaaaag gtatatgacg aagataaagg ggattatact
     4741 tacaacatga aagtgggagc tattacaagc acacttccta aaatagtaga tactagttca
     4801 agatatacat taatgcaaat agtgccaatg tctatggtgc aggaaatatc taaaaattat
     4861 gatgaagtaa gtaggttacg ttcaaatagg cttataggat tatttaaagt aaataatcgt
     4921 aaggacattt ctagagtaag agaaaaatta gagtcaattt gtaataaaga ctatggttcg
     4981 ggagactata gtattgagga tgttttagaa agtgaaaaac aagatgctag tggaagaaag
     5041 accatgaatt tgatagtagg ctttattaca ggtttattgg ctatgattgg attatcaaat
     5101 gtacttgcta cagtgtcagg aaatattaga agtagacgac aagagtttgc tatgttgcgt
     5161 tctgttggat tatctccaga tggcataaaa aagatgcttg ttttggaagg tttgttctta
     5221 ggaattacac cattactact tagtattcct gtgcaaatag gaatagtgta tacttttcta
     5281 cgtataaatg aaatatattt tatagagtat ttaccatttg cacctgtatc tacaataata
     5341 ggttttacca tattgatact ttttattgta atagcatcat atatgacagg atacaagcag
     5401 ttgaagaatg aaaatatagt agaatctata aaaaatgaaa caatataaac aaaagctatt
     5461 tggacttatt taagggtttc gatttaagac tacttacaag tatagggata gttaagttgt
     5521 ttatttaata aaatcttaaa tgtaaataga agtatattac taaaataata gataacatag
     5581 aatatagata tggctggatt ataggtattt tacctataat ccagcatttt atgtgtaaaa
     5641 tttaattatg aatcttgcac caccattatg gcaattttct gcatgtagag taccattttg
     5701 acgttctata atagactttg atagagcaag tcctatacca atactgtcct tactagcatt
     5761 tttaccacga taaaatctat taaaaatata tggaagttct tcctgagcaa acccattacc
     5821 attatcttct atgataatct caacatatag aggattttga ctataataag caactatata
     5881 tccaccatct tctatatgct cactacaatt tttaagaata tttaaaagag cttctaaagt
     5941 ccaattaata tcgatttcaa attctaccga aggagaacct tcgatagtaa atgtctggtg
     6001 tttttctctt aaagaacctt ctataggctc aattgcataa gtaagaagag aatgtacatc
     6061 tataagtttg ctttcaaata ttaaagtact tgtatccaat tttgataatg taagtaaaga
     6121 tgaaactagt ctctcaagtc ttgagatttg atttgtaagc ctgttaatat aatctatttc
     6181 ttcatctgga caattttctg aaagtaattg tgccataagt gacatagaag taattggagt
     6241 cttaagttga tgagcaatat ctgcaaggtt atctgaaaga gtttgtcttt cttttaaagc
     6301 aaattctttt gaacaacgaa gttccccaac tgttttatat atctcatctt caaggtgaga
     6361 aaattcatct tcacatcgta aaagtacaga ctcttttcct aaattcactt cttctaagta
     6421 ttgagtaaga ttgtttattc tactcaattt ttgcttattt gttctatgtt tgattatata
     6481 taaaactata gtcattgttg aaattatacc aaacgatacc actagaaaat aaaaaaagtt
     6541 tttactccaa aatgtacttt tagaatatcc atgttttgat aaaatctcat ttcctgtttt
     6601 aaaatcctca tcactagaat ttttcaatga ttttagtata ctttctgtag acttaggatt
     6661 gtactcatat gatgcaccaa gagccttaga cagtacattg tattcattaa aagaagttat
     6721 agttgttaga attacactca ataaaaaagt tacaattaag cagaatatta taaaaacaac
     6781 tgtactgttg atatttcttg gagttttcac tagctgtcct ccattctata tcctatgcct
     6841 cttaatgttt ttatacaagg tgtatttttt aatttttctc taagacgctt tattgtaaca
     6901 gtaagagtgt tgtcatttac gaaattacca tctacatccc agagcatttc tagtaacttt
     6961 gtacgagtca tagctctatt tttattttcc ataagactta gaagaagctt atattctcct
     7021 gctgtcaaag gtacttcaac attatctaaa tatgctttga cttcaacttt atttaagctt
     7081 atatttccac atgtaatagt attgttagta ttggaatgag ttcttctaag aactgcatta
     7141 attctggatt gaagtactga aagcaaaaat ggtttaacca catagtcatc tgcacccata
     7201 tctaatccac gtataatgtc tttttcatca tcacggactg tcaaaaatat cactggagta
     7261 tcttctatac ttttaacata ttggcaaaac tcgtatccaa taccatcagg tagatttaaa
     7321 tcaagcagaa ttaaatcaat agttttatca aaattttttt tagcttcagt aatcgtatta
     7381 taacaacata cagtgtgatt tttcttttca agggaagttt ttattccaaa tgtaattgta
     7441 tcatcatctt ccaaaagaag tattttagcc atttactttt agtcctttca gtaatttagt
     7501 tatttcatta actaagttat tagttttatg tttaatattt tttatgttag agatagaagt
     7561 tagagataga aaaaattatc atttaagtat ataaacttta atttataaac ttaggtttaa
     7621 gctttaattg aatctattaa aagagcaaga gttgtttcaa taaatctttc ctcatctata
     7681 ttaaagttgt tcaggtatga ttttcctgtt acagatagta aattaaaaaa tcctgtaagt
     7741 gtaaatattg atgaatacat aagagtaggt atttcaatat catgcctaat acttctatca
     7801 ttttttccaa tttcaaacat ttcttgtatt tcatcaaaca taaatttatt aaaagaaaaa
     7861 aacttttctt taaacagcat atcagtatcc gatttttcag agtttacaat accaatcatt
     7921 cccataaggc ataatagaga aggttctaca gagtaaaatt tatgaaaagc aaagtatgaa
     7981 agttttattt tttcaaaccc tgttttgcct tttgcacttt ctattttaac attatcccaa
     8041 agtcttttat atcctcttaa gacaacagca aagaataaat cttctttaca tgaaaagtac
     8101 ttgtatatgg tacgtttagt atattcaact tcctttgcca actcactcat agttgtacta
     8161 tcaaatccat taagacaaaa taatttttct gccttatcaa taatctcatt ttctctttgt
     8221 attttttctt tatctctttt tgataattct gacatataaa ttattccttt ctattgataa
     8281 aaaagtatac tgatggtata cttatgtaaa tataatattt gtatacctat agtatacttt
     8341 aataaaaaat aaaattaata tcaagtatta taactattta taactttatt aattaattat
     8401 ataggtaaaa taccaaataa ggggaattta tttatgaaga taaaagaaaa tgtattaatg
     8461 ttagatagta ctcgtggttc aaattgtttt attgtgttcg ataaagaaat tattcttata
     8521 gacacaggtc ttccttttat ggggaaaaat ataattaaag aattaaaaac acttggcatt
     8581 aaacttacag atattaagca tattttatta actcatcatg atgttgacca tatatctaat
     8641 attaaattat tgaaagagaa aacaggagca aaggtatggg ctcatgtaga ggatatacct
     8701 tttattatag gagagaagga tagacctggg tttaagaagt ttattggaaa ggcaatttcc
     8761 agaaggctca ttaaggatgt tgaaccttat ggagagaata tgcagattgg gaatataaaa
     8821 attatccata ctcctggtca tactccaggg catgtctgca tgatttttga agatgtatta
     8881 tttgcaggtg atttagtcaa aaataagaat ggaaatatag ttccttaccc taatccatgg
     8941 aattgggatt ataaaaagat gatgaaatct ataagagaat tggataattt aaaatatgaa
     9001 tggatatgta tgtctcatgg aactccttgt attaaataat aaaaataata agttgtaata
     9061 tatcctatgt tatctattga ctatatccaa tattggtgat aatataatat ccaagataga
     9121 atataggtgg tgaattttta tgatacgcac tttaatttta tattatctaa atattaagcc
     9181 aacacatggg tacgagattc aaaagttttt acaagtatct ggtgcagata gatggactaa
     9241 aatacaatct gggtcaatat attatgcatt ggcaaaatta gaaaaagatg gaggagttag
     9301 agttttaaga gaagaaaaaa caggagctag gattcgtaaa atctatgaga ttactcaaag
     9361 tggtaaatta gaattaaggg aagaaataca aaaagagctt cagatgccaa tagttccaac
     9421 cggttcaaat aaattcctat tacataatat tttggatgta ttacctaagg atactttgca
     9481 aaaaaatctt gaaaaacata taaaatatct tatagagcag aagaagtatt gggaaaattg
     9541 gaaagagatt aaaaaaattg ataaaaagag tcttgcaaca gaaaaaattg catttgatat
     9601 gactatagat aatctgaatt atcaaatttt gtggcatgaa gaaatattaa ataatataga
     9661 taagtatata tctgttggtt gcgagacaca gaatataatt aaatctatag acttttcaaa
     9721 tatagaagaa gattttttat ttaaatctga tacgacaaat gagttattag aagttcagag
     9781 acttagagat gaaataatta ataacccaga taaagctata gaaaatatag ataagataat
     9841 attgaaatta caaaataagt aataaatttt attacaataa cacgagtaaa atactcgtgt
     9901 tttaaaaaaa ctatatatac aacattggat atataatgtt ggttaaatat taggagggtt
     9961 ttataaatga gtaatgtatt gttagagatt aaaaatttat ctaagaagta taacaataag
    10021 gaagttgtat caagagaaag ttttaagtta ttggaaggag atattttagg ttttattgga
    10081 ccaaatggag cagggaaaag cacatctatc aatatgctta ctacgattgt gtctccagat
    10141 agtggaagca tatactataa aggaaaagat attaagaatc aacaaaatat ttttaaaagc
    10201 tcacttggag ttgtaccaca agaattagca atatatgaag atttaagtgc gtatgataat
    10261 gttaaatttt tttgctcatt atatggattt aaagggaatg aacttaaaag gagaactaaa
    10321 gatgctttgg aatttgttgg tctttgggag aggaataagg agtttccatt aaaattttct
    10381 ggtggaatga aacgtagact aaacatagct tgtgcaattg cgcatagtcc aaagattctc
    10441 atcatggatg agccaacagt aggtatcgac cctcaatcaa gaaataaaat tttgaaggca
    10501 attaaagaca ttaatgctaa tggtacaaca gttatatata ctagtcacta tatggaagaa
    10561 attgagacct tatgtaatag aattgtttta atagataaag gagtaattat agaagattta
    10621 gacaagagtt tatataaaaa taaatattta gaatttggtt gtaagacatt agaagatatt
    10681 tttctatatc ttacaggtac agatttgaga gatttggagg aataaataga tgagatattt
    10741 tttaccattg tttaaattag gaataaaacg aagaagtaaa gattttttta ttctgtttta
    10801 caatattgtt tttccagtaa taatcataat gctattagga tatttgacat caaaatccta
    10861 tggtgaggaa tttacatcat ataattatta tactattgtt acaataccat tttgtgttct
    10921 tatgggaatt attagtgttt catatgtggc acaagatgaa aaaatttcta acacatcata
    10981 tcgttatatg atttctccta tttcaatagt agatttagtt atttcaaaac ttttttcttg
    11041 tacagcagta ctttcaatat gcaatttgat aactttagcg gtgttaagag gaatttttca
    11101 tatgagtttt ggtgggaaat ttttagttgt tattttcttt ttaatgtgtg aatctattgc
    11161 agtagtatct atgggtttgt ttttgggtct tgtttgtaaa aatttagata tgttacgtaa
    11221 tatcattaat atcccaatag ttatttttgg tttcttagga ggagtgttct ttccagttag
    11281 ctcattgaat ccagttattt cattaatcat taatatatca ccattgacat ggattaatag
    11341 aggtatagtt gcttgtatct atgataataa tttacaactc ctttttaata tatcatgcat
    11401 atttgtagct gtaagcatat ttattacaat acttacaatc aaatttttta aaaaggaggt
    11461 ctttatatag tgaatgtaat aaatgttttt aatagtaact taaaaagaaa tataaataag
    11521 cgattagtgt ttttagttac acttttattt cctataatta tagtaatttt aggagtattg
    11581 gcaaattaca taagtaaacc atcttttaat attggtattt tagatgagta caaaaacgaa
    11641 cgagttctaa aggaatctga aaaaaggacc tatgaaaata caataatggc tttagaaaat
    11701 actcaaggga ttaatacaga gattgcagat tttaaaacaa ttaaaactga tattataaca
    11761 ggcaagtatt cagctgtcat atgttttaat aagaatggtt ttaagttata ttctataaaa
    11821 gataaaaata cgaatgatac attaaagtat ttaatagaaa catataaaaa agacccagtt
    11881 cctgtagata taagcaaatt acaagaaaat actttaggag ttttacaaag aataattgca
    11941 tttatcgtat tatttttaat gataacatct actgtaacag catcaatgat tattaaagat
    12001 aaaaactcag gtacatttac aagagtgctg tattctccac aaaatataag aggatatgtg
    12061 ctaggtaata tgttatacaa ctttacaata acgtattttc agtacatagt ttcaatatct
    12121 ataattaagt tatttaatat tgatattggt atggattata tagatttaat tttaatggga
    12181 gtatggatat cggctttggc aacttctttt ggaacatttg tatcttcttt attcaataaa
    12241 gaaatgcatg caaatttgtt ttctacatgt atatcgctta tattatcact tattggtggt
    12301 tcatttattt cagttgaaaa aatgccaaca atgttacaaa aaattagtat tataagtcca
    12361 acacaatggt ttattagttt tgtgacttat atagaagaaa gagatggaag cttttttaat
    12421 atgaattata tttgtatttt gacatctata ataataattt tagctttgtt agctatgaaa
    12481 tttattcaaa gaagtaaata ttcaagtaat aggaattatt aaataagata gaaaatgaat
    12541 agatgtaatt aaaacaaatc agcatataaa aatataaata tggttctata tcttataaat
    12601 tgggtttaat tataaaaata gaaattatat agtgtaaaaa ataatgtgaa aaatttttta
    12661 tttataatag aattgtggta tgagcaaatt aaatttattc tgatatagct cttttttatt
    12721 tgtcttaata gaaatatttt taagactctt ccctagaaaa accaactaat gtatattaaa
    12781 ataatattta gggagtggaa aaaatgagat tagaatcgtt agttaaccaa aattatgata
    12841 atttaaatga aaatgataaa attattatta aacaaatatt gatttgtcaa agagaatacc
    12901 gtaatttttc gtgtgaaaaa attgcaaatg aatgtaatgt ttctagagct actttattaa
    12961 ggctttgtag aaaaattgga ttagattcat tctctgattt aaaatattta cttaaaaata
    13021 atcaactaga atttgttaat aatgataata attttcatga tgtttgcgag aattatcgac
    13081 tattaatgaa tgaattacaa aaaatatcat ttcaagatat ttgtaaaata atatatgatg
    13141 cagaaacaat ttatatttat gggacaggta acgaacagaa aagtttagct caggaaataa
    13201 agagaatatt tctttcagta ggtaaatgtg ttattgactt atttgatata ggtgaaattg
    13261 aatttatgag agaatcattt caaaaaagtg atttgtttat tatcatttct ttgtccggag
    13321 aaacgaaagc aggaattgaa attgcaaaat ttataaacaa tcatatacat acgatttctt
    13381 taactaggct tgataataat acaattgcaa cactttgtga gtataatctg tatgttgaaa
    13441 cgcaaaaact tgatacatta caagctatat cttatgaagt cgtaggagca ttttatgctc
    13501 tattagattt gcttcatatg aattacatag agtatagaag gagaaaagaa tgaaaattga
    13561 agggcttttc aataaatatt ataattcatt tacagcaaat gataagtata tctgtgagtg
    13621 tattcttaat cataaagaag attgtattaa actttctata gatgaatttg cagacaaata
    13681 ccatatatct acaagtgcac tttctcgttt tgctcaaaaa ctacaattac ctggatatag
    13741 tgaattaaga gttattatac gcttaaatga aacagaagta gaatataaaa atcaaagtaa
    13801 acaccaatta atggattgtt ataataaggt tatagatgat atagagaaga aggattgttc
    13861 gattatgttt gaaagaatga aaaaggcaaa tcgtataatc gtctttggag aaggttattc
    13921 tcaggaaaga gttgctaaag agatgaaaag gattttctta ccaacaggga aaattatata
    13981 tgatgtttat ggctatgata tgattcaggc acttaatcat tttataaaaa cggatgatat
    14041 gattttcttt atttcattaa atggagatgc atctacaatt attgactttg ctaaaaaaat
    14101 gaaaatgaaa ggaatttata tggtttctat tacaaaaatg ataagtaatc ctttagcaag
    14161 attatgtgat gaaaacctat atattcaatc tatatctatt tctgtagata aacaactgga
    14221 ctacgatgta acaacacctt attttatttt aattgaactg ctttatatta aatacaagat
    14281 gtatttagaa agtgaacata ttccccgaaa atgaaaattt gtgctaaaaa tgaacatatt
    14341 ttttaaaaga tatcaaaata ttgatatctt tttttttgaa tgctattatg attttgatag
    14401 taagaaagga agatgaaatt gtacaaatta ttaatacttg atatagatgg tacgcttaga
    14461 gatgaggtag atggaatccc agaatctgca aagcatgcta tacgtttatg tcaaaagaat
    14521 cattgcagtg ttgttatttg tactggaaga agtatgggta caatacaaga tgatgtttta
    14581 tcattagggg ttgatggata tattgcagga ggaggtaatt acattcaata tcatggtgaa
    14641 ttactttata atcaatcttt taatcaaaga ttgataaaga aagttgtttg tttattaaaa
    14701 aagagagaag ttgctttttc gattgagagt cgggaaaaag ttttcatgaa tcaaaaagcc
    14761 aaagaaatat ttgaaacaat gaatcagttg aaaggaacta attcttgtat aaataaacaa
    14821 catatccaag aaaaaataac atatgaaaat aacattgaag agtataagtc acaagatata
    14881 cataaaattt gtttatggtc aaatgaaaag gtgtttgatg aggtgaaaga tatactacaa
    14941 gataaaatgg aactagcgca gagggatatt tctagtcagt attatgaaat tattcaaaaa
    15001 gattttcata agggcaaagc aattcaaaga ttacaagaaa gattagggat aactcaaaaa
    15061 gaaaccatat gttttggtga tggtcaaaat gatatcgtca tgtttcaagc atcagatatt
    15121 acaattgcaa tgaaaaatag tcatcaacag cttaaagata tagcaacatc tatatgtgag
    15181 gacatattcg ataatggaat ttataaagaa ttaaaaagaa gaaatatcat ataaaaagga
    15241 gtaatttatg gaaaagaaat ggtggcaaaa agaaatcgtt tatcaaattt atccaagaag
    15301 ctttcaagat agtaataatg atggaattgg agatttacag ggaattgttt ctcgtttaga
    15361 ttatttaaaa gatttaggta ttacatcatt gtggttatgt ccaattttta aatcacctat
    15421 ggacgataat gggtatgata ttagtgatta tttggatatt gcaagtgaat ttggaagtat
    15481 ggaagattta aaagagttaa ttaaagaaag taaagataga ggaattaaga ttatattaga
    15541 tttagttctt aatcatacga gtgatgaaca tccttggttt caagaagcat taaaaaaccc
    15601 agaaagtcct tatcatgatt attatatttt taaaaaagga aacgatttgc caaataactg
    15661 gaggtctgtt tttggcggaa gtgtttggga aaaggtagag aatagagacg aatattattt
    15721 tcattctttt ggaaaaaaac aacctgacct taattgggaa aatccaatat taagaagtga
    15781 actttaccaa atgattaata cctggtctca aatgggagtg gcaggattta gagttgatgc
    15841 tattacattc atcaaaaaga atttaagttt tcaaagttta gaaagtgatg gtgttgatgg
    15901 tcttgttaaa tgtacaaaag cagttagaaa tcagcctgga atagaaaaaa tgctttatga
    15961 attaaaagaa aatacgttta aaaaatatga ttgtatgact gttgctgaag cagcaggtat
    16021 tagctatgac cagctaggtg aatttattgg agaagatggt tttttttcaa tggtatttga
    16081 ctttaaacat gctgacttag atgttgcttc tggtagtgag tggtttaaaa gaatttcatg
    16141 gacaatttca gatttaagaa aatacatttt agatagtcaa atgtatgttc agcaacatgg
    16201 atgggcagct aattttattg aaaatcatga ccaaccaaga gctacaacaa agtatctttt
    16261 gcaatacgaa aggaatgatg atgcagtaaa aatgttggga gcaatgtatt tctttttaag
    16321 aggaacacca tttatttatc agggacagga attaggaatg acaaatttta aaagaaatac
    16381 cataaatgat tttaatgatt tatctagcat tgaccaatac tatcgttcaa tagatgaagg
    16441 ttactcagaa gaagaagcgt tagaatttgt gaatttaaga agtcgagata atgcaagaac
    16501 accatttcca tggtcagata aagaaaatgc aggtttttca gatacaacac catggttaaa
    16561 ggttattgaa aattataaag atattaatgc acagagtcag attaacaatt cagaaagtat
    16621 tttagaattt tataaaaaga tgataaagtt gagacaagat agtgagtatt ctgattgttt
    16681 gatttatggc tcaatagaac ctattgatat agaaagtgac aatgttattg cgtatagaag
    16741 gcaattagat ttggtagtta ttgattgtta ttataatttt tctgataaac ctgtagaaat
    16801 aaaaacagaa gaaaaatatg atattgtatt ccaaaattta gatgatataa atctactaaa
    16861 taaaacagtg agtttgaaac catttcaagc agtattattg aaagtgaggt agaaaaatga
    16921 ataaatataa taaaatagca aatgagctta ttgaaattat tggtgaaaat aatattattt
    16981 ctattacaca ttgtgccaca agattaaggg tgatggttaa agatagagaa attattaatg
    17041 ataagaaagt ggaaaaggtt aatgaggtta aaggtgtttt ctttacttct ggacagtatc
    17101 aaattatttt aggaacggga atagtcaata aagtttatgc agaagttgaa aaaatgggat
    17161 tgaaaacttt atcaaaaaaa gaacaagatg aattagttaa aaataatgaa actggattta
    17221 agaaagttat gagaactcta gcagatatat ttgttcctat tattccagtt attgcggcaa
    17281 caggattatt cttgggatta aagggttgtc tgtttaatga taatgtactt ggattatttg
    17341 gaatgtcttc tgcaaatatt ccattgtata ttcagacact tgtttcggtt cttacagaaa
    17401 cagcatttgc ttttttacca gcaattattg tatggtcagc atttaaggta tttggaggaa
    17461 cacctgttat tggacttgtt attggactta tgttagtttc cccaatactt cctaatgcat
    17521 attctgttgc tgacccatca aatgaagttg aagcaattat ggcatttgga tttattccta
    17581 ttgtaggctg tcaaggaagt gttttaacag ctattgttac agcatttatt ggagctaatc
    17641 ttgaaaaatg gttccgtaaa catatgccta atgtattaga ccttattttc acaccattct
    17701 ttgttatgct aataacaatg ttagttattt tgttaggtgt agggcctata atgcatacaa
    17761 tagaattaaa aatggttgat atcatcagct tattgattgg tttaccttta ggaattggag
    17821 gatttattat tggatttaca tatccattag ctgttattac tggcttacat catacttatg
    17881 taatgattga aacatcacta ttagcaaata caggatttaa tgcacttatt actctttgtg
    17941 caatgtatgg atttgcgaat attggaacat gtctagcatt tatgaaaaaa tctaaaaata
    18001 atcaagttaa acaaactgca gttggagcaa tgctatctca actttttggt ataagtgaac
    18061 ctgttttatt cgggattcag cttcgatata atttaaagcc tttgattatt atgtgtgcat
    18121 cttcaggttt aggagcagca atattatcaa ttttacatat tcaatctaat tcatatggat
    18181 tagcagtttt accttcttat ttaatgtata tctatgatgg atataattta atcacatact
    18241 tattagtatc aatatttgta gttgtattct gctttattgc aacatgttta tttggagtac
    18301 ctaaagaagc tataaatgaa gatgaagacg aagaattagt gtttaatgaa aataatgaaa
    18361 actttgtgtc tccagctaaa ggtaagattg ttgcattaga aaatgtacct gatgaaactt
    18421 tttctaaaaa aatgttaggt gatggttttg ctattgatat tatagatgga aaaattgttt
    18481 cccctatttc aggtaaatta gaaacagtat tttcaagtgg acatgcattt gggattaaag
    18541 gaactaatgg agaagtatta attcatgttg gaattgatac agttgaatta aatggagatg
    18601 gctttgatgt tgctgttaaa caaggagata tggttaaaca gggggatgtt cttgttaatg
    18661 ttgatttaaa gagaatacac gaattaggaa agtctacttt gacaatggta ttgtttccag
    18721 atggtaagaa ggtaaatatt ttggatataa acaaggatgt aaaaataggt caaagaatat
    18781 gtgttgagta gtaggtgaca cgtaatgatt ataaaagaag attatcaaaa tcaattcaat
    18841 attttcaaag tatgagaaat aattcatttc atattaacta ttggtaagtt caaggtataa
    18901 gaaataattc atctcatatt aactattggt aagtaaaaaa gagctttctc atagcaaatt
    18961 taatttgctc atgcgacagt tctttttata ttgtataaat ttttagtgta gccatatctt
    19021 agtattaaag cacatttttt gtatgtaatc aataaaacaa cttacatggt atccatcagt
    19081 agttgcgtga tgcactgtta tagaaagagg aagaagtatt ttttcatctt tttgatagta
    19141 tttacctgct tcaattgtag ggaaaaaata attttcaaga ccatgattat gtacagtaaa
    19201 actcttaaag ctaatccatg gaaggcagga aacagtatag cagtttcttg gcggatttgg
    19261 ttttgctaga aaaccatgat tttttccata ttctttaaca tcagctaaat aattttcata
    19321 gaatattgag aaattttcat catattctgt ccatattgat gagaaagtct tattatcatc
    19381 atgaaaaatc gcataaagtg gcgttaacgt gttccaatat ccgagttcat cttcatcata
    19441 tgcgattttt agttctctat atcgatttaa gcaagtagtt attgtatata aatacgctgg
    19501 ataaaattta atatttcttt ttttcgtttc cttaagcaaa ttagtaatat ctatttctat
    19561 agtcaaatta aaccctacat ttgtcatttt tgaaaagtag taaaaatgtg tatatcgttc
    19621 ccaattttgt aaatcaattt tatggaattt ttgtttattc atataaaatc accatgtcct
    19681 ttataatatt tatttgatta tcaatgtctt tatttgtaag ttttaaaatg gaagcagatg
    19741 ttttcatgct atcaaataag aacattatgt gtattgcaac tgatttagaa tcacattttt
    19801 taaattcact tttagattgt ccataatcga ttaaatttga aagcattgtg atagctgaat
    19861 tgtatctttt attcatataa tcacgttggt ctttttctac aaaagcaaat tcgtgtattg
    19921 caaaatcaaa tccacaatat ttgccattta tgtcagcctt ttgttgttgg aaaaaatatt
    19981 ctagcatatg cttagctgat atattttcct tgataccttt atccaacaaa ttttgagagt
    20041 agtatttatg agtgtttaat atactaataa aaatttcttt tggtgaatta aagtgtctat
    20101 ataggccacc acggcttaaa ttacatgctt cacatatatc tttcattgtc acattactat
    20161 atcctttttc agaaaatagc attattgatg tatcaatgat aaattgttta gtttgttctc
    20221 cttttgttgg catatgtttt aacctccttt ttaacgacat aagtgtcact tttaattatg
    20281 agacacttgt gtcgttttgt caagttaaag ttatatacca taaaataatt acaatagaat
    20341 aaggttaatt tataaaatag ttgaagaata caaaagaata taatggaact gatttatttt
    20401 taagttgatg taaaaaataa ttaaataatg ataatattat tgacttttaa aacttacatg
    20461 tgtaatatat aaattactat caattatact gcttacatat ttttatgaga tacttaaaaa
    20521 cataataaaa acatagatta gctatgtcta taagagtatt ttaaccttaa agcttacgta
    20581 tgtaatatat taaatgataa aggtggttta tatgagaaaa aataaaaaaa caaaaaaaat
    20641 gataattttt ggttttctaa tgtgtttgct tatagttatt attgtttttt tagcaaaatt
    20701 catactagat ggactcaata aaaacaaacc agatgaagtg tttaagcagt atatgtcttt
    20761 tgcaaataaa aaacaatatg agaaaatgta tgatttatta gatgaaaaga gcaagtctga
    20821 aaataaaaaa gaagattttg ttttaagaaa taaaaaaatc tatgaaggaa ttgatgcaca
    20881 cgatataagt attaagatta atgatattaa aaaaaataaa aataatgata aagtaataaa
    20941 ctatgattct aagatggata ctttagcagg tgaaatttca ttttcaaatg aagtactatt
    21001 aactagagat agacagaaaa attataaaat aaaatggcag tcaaacgtca tatttccaga
    21061 acttgaagaa gataatactg taagggtatc aaagctgaaa ggtaagagag gacgtattct
    21121 ggataggaac ggtgtaatga ttgctggtca aggtcttgca tctatgatag gattagttcc
    21181 tggaaaaatg agtgataata tagaagactt aaaaaaatta tctactttat tgaatgtatc
    21241 agttgaacaa attgaaaaga aattaaatgc atcttgggta aaagaaaatt caatggtacc
    21301 tataaaaact attgaaaaaa taaaagaaaa tgcagatggt actgtgaaag aagaagataa
    21361 ggaactacaa gagtcacttc ttagtattcc tggagttaaa atatctaata cagaggttag
    21421 agtatatcca tttggtgaaa aaactggtca cttaactggt tatgttcaaa atgtaaatgc
    21481 agagatacta aaggaaaaag agggtaagag gtacaacgat aatagtataa ttggtaagat
    21541 tggtttagaa aatttactgg aagatagaat acgtggtatt gatggatatg agattataat
    21601 agctgataga tatggtgaca agaaagaaac tttagtaact gagccaaagg tagatggaga
    21661 agatgtaaag ctaactatag attcaaaact acagagtaaa ctatatgacc aaatgaaaaa
    21721 tgataaaggt tgtgcagtag ttatgaatcc taaaacagga gaggttttat cacttgtaag
    21781 tagtccgtcg tacaatccaa atgagtttat attgggaatg tcagaagata gatggaatga
    21841 actcaataaa aatgaaaata aaccaatgta caatagattt aaagcacgat tatgtcctgg
    21901 gtcttctttt aaaccagtta ctgctggtat tggtataaca acaggaaaga ttaatccaaa
    21961 tgaaaacttt ggacacagca gtctaagttg gcaaaaagat tctagttggg gaagttataa
    22021 ggtaaccacg ttaaaagatt atggaaatac tgcaaatatg aaaaatgctt tgatttattc
    22081 tgacaatatt tattttgcta aggcagcctt aaaaataggt gaagatgtat tagcaaaaga
    22141 gttgttgaag cttggatttg atgaaagtat gccatttgaa tttggattgt cttcttcaaa
    22201 atttggcact gacaataaat ttgagactga aatacaatta gcagatactg gctatggaca
    22261 aggtaagctt cttgtaaatc cagtacatac ggcatcaata tattcagctt ttgtaaatga
    22321 tggagacatg ataaaacccc atcttgaatt taaaaaggct gaattttgga agaaaggagt
    22381 gttttctaaa gaggctgtaa aaattattcg tgatgactta cttcaagttg ttgaaagccc
    22441 tgatggtaca ggtcatagtg ctatgattaa aggaattagc attgctggga aaactggtac
    22501 agcagaaatt aaagcatcaa aagatgatgt tacagggaca gaacttggat ggtttaatgc
    22561 ttttactgta gatgaaaata gtgataaaca atatctaata attgctatga ttgaagatgt
    22621 aaagagtcgt gggggaagtc attatgttat tcctattgtt aaatcagtat ttgaagatta
    22681 aatttactca atttggaaat taatcttact cagttgagaa taatatttta gatacattgt
    22741 ttttagactt cttagtaaac tctaaaataa ttaaagaggt tgtttcaaaa tagagataga
    22801 cctctttttt attgttgaaa tgatgaaaat atatttaact tcatacaaaa aattaaatag
    22861 tggcaacaat tgaggatgtg agggagagtt gcca
//
LOCUS       DAEPXF010000425        15599 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain HB27
            SAMN13376389-rid12479423.guided.090, whole genome shotgun sequence.
ACCESSION   DAEPXF010000425 DAEPXF010000000
VERSION     DAEPXF010000425.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13376389
            Sequence Read Archive: SRR10518432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 15599)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15599)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001069624.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001069624.1
            Genome Coverage       :: 315x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/15/2021 01:54:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,165
            CDSs (total)                      :: 4,076
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 89
            rRNAs                             :: 10, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 10 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 19 of 69
            Pseudo Genes (incomplete)         :: 44 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 10 of 69
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15599
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13376389-rid12479423.guided.090"
                     /strain="HB27"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="China: Hebei"
                     /collection_date="2017"
     gene            274..543
                     /locus_tag="KRL67_004122"
     CDS             274..543
                     /locus_tag="KRL67_004122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010963800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HBH1524887.1"
                     /translation="MKAILTVIGKDKPGIVAGISDELYKQNINIVDISQKILQDYFTM
                     IMIVDLEKSLNSFEKTVEDLVERGKKLSVDVKIQREEIFNSMHNL"
     gene            569..1924
                     /locus_tag="KRL67_004123"
     CDS             569..1924
                     /locus_tag="KRL67_004123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009170073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PFL family protein"
                     /protein_id="HBH1524888.1"
                     /translation="MNVGNILETIKMIDEEQLDIRTITMGISLLDCIDPDGDKAREKI
                     YNKIMSSAKDLVKVGKDIEREFGIPIVNKRVSVTPISIIAGATDENDYVKFAQTLDKA
                     AEDLGIDFIGGFSALVQKGYTKGDKILIKSIPKALASTNKVCASVNVGSTRCGINMDA
                     VKEMGEIIKETAELTKDAKGFGCAKLVVFCNAVEDNPFMAGAFHGVGEADRIINVGVS
                     GPGVVKRALEKVKGEPFDVVSETVKKTAFKITRVGQLVAKEASSRLDVPFGIIDLSLA
                     PTPAIGDSVANILEEMGLEVVGTHGTTAALALLNDAVKKGGVMACSHVGGLSGAFIPV
                     SEDAGMIDAVIKGALSIDKLEAMTAICSVGLDMIAVPGNTTAGTLGAMIADEAAIGMI
                     NNKTTAVRIIPAPGCDVGDMVEFGGLLGRAPVMPINTNSSELFTQRGGRIPAPIHSFK
                     N"
     gene            complement(2389..3429)
                     /locus_tag="KRL67_004124"
     CDS             complement(2389..3429)
                     /locus_tag="KRL67_004124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBH1524889.1"
                     /translation="MSEILLGTGLLLLVLCCFTIFSYKAPYGMKAMGALANAACATFL
                     VEAFNLSLLGDVLGMEFFRSVGAANGSLGGVAAAILVPLALGVSPVYAVLIGLACLDF
                     SILSGFVAGYFVSFLIKFIEKKVPPGVDLVVVILLCAPLSRLIASNVDPLVNSTLLQI
                     GQVLIEATNISPIIMGIILGGLITVVGTSPLSSMALTSIIGLTGLPMAIGALGAFGSA
                     FMNSTLFAKLKLGSKKDIISVAIEPLTQSDIISANPIPIYSTNFFGGAFSGIIVALMG
                     LVNMTPGTATPVAGFAIMFAYNPVQQVLITAGLCALFNVIAGFLGSKIFSTFKVLTAG
                     EIRGTDVEEEIA"
     gene            3810..4445
                     /locus_tag="KRL67_004125"
     CDS             3810..4445
                     /locus_tag="KRL67_004125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH1524890.1"
                     /translation="MKKVKYTRLVILGILIYLVFNILVMIISKNVDTLVLKNEKVDAK
                     IHVKGTIEKDANDQSNYLSTYISKEDSNNFEKNQNVIIEYHEQKLDAKVYKIYENNNK
                     IMVKLKISNQIVANQDTSVEEFDIIYNQMECLKIPKTSIKTKDNKRGVYVIDEQSQSV
                     KFVILEGITYENESSVFVDYYKNDINGVTSVNLYDKIILRPNSINTNIRIK"
     gene            4455..5159
                     /locus_tag="KRL67_004126"
     CDS             4455..5159
                     /locus_tag="KRL67_004126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HBH1524891.1"
                     /translation="MGSIKDNLSEIRNKIDKAAKKVDRDSKEITLLAVTKTVDVDLVN
                     EAMREGITSVGENKPQELARKYDIIGDKLNWHLIGTLQTNKVKYIIDKVCMIHSLDRI
                     ALCEEIQKRAEQINREIDCLVQVNISKEESKHGLDREEVVDFVKEVSEKYKNIKIKGL
                     MTMAPFIEDEDEIRGVFKGLKDLSKQIKELNIPNVGMDVLSMGMSHDYEIAIEEGATI
                     VRVGTSIFGERNYNKK"
     gene            5199..5657
                     /locus_tag="KRL67_004127"
     CDS             5199..5657
                     /locus_tag="KRL67_004127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HBH1524892.1"
                     /translation="MSNGIISKFKNWIVDEEEDYIEDEYESGMDDIVQEEEMNSGFST
                     AKANKIVNLHTTSQMKVVIVEPKVYDEAATIADHLKQRRAVIVNLEGLTNSEVRKSIF
                     NFMNGAVYVLDGSIQKVSKSIFILAPNNVDIDANMKKELESKAFFPWQNK"
     gene            5676..5933
                     /locus_tag="KRL67_004128"
     CDS             5676..5933
                     /locus_tag="KRL67_004128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HBH1524893.1"
                     /translation="MGTIRIALYYLLDIIAWMIVIKSLMTWFPNGRQSKIFDILENLT
                     EPIEGPIRSIMYKYTNGPIDFSPLIAIVLLMFLRQLVLVIF"
     gene            5943..6728
                     /locus_tag="KRL67_004129"
     CDS             5943..6728
                     /locus_tag="KRL67_004129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HBH1524894.1"
                     /translation="MDKLKLTNHIKDIDLKNKMFKVIDKANSCIKNYDVKSTEFLNPY
                     EVKNAVAILNSTNEIKYSVDGGYEQAERSTVFIYPFYMEYEDIEDTLRFLQIEGNFKF
                     KNISHKDYLGSILSLGIKREKIGDIIIHDSFCQVVVSSDICDFIIVNLEKVSRNNVIV
                     KEISRENIVNSSSKYKEVSFTVSSDRLDCVISGIYNISRQDSAKYINGEKVHVNYEKI
                     TSTSKIVKNDDLISIRGKGRAKVTQIGDITKKGKIKVQARLIV"
     gene            6763..7290
                     /locus_tag="KRL67_004130"
     CDS             6763..7290
                     /locus_tag="KRL67_004130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HBH1524895.1"
                     /translation="MLTPIEIENKEFKKGLRGYRDEEVDEFLDIIKEDYESLCRENTA
                     LKEKLGLYQDQVNKYENIEETLKATLITAQSAAEDTCSAANKKAKIIVEEADLKARQI
                     IEQANNRVIEIRKEYDALVKEFKIFRNKFKSLLEDEIRSVDEIFYDVDEKCVNAFEGT
                     TVYNYSNDEAATTLE"
     misc_binding    7465..7697
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            7789..10896
                     /locus_tag="KRL67_004131"
     CDS             7789..10896
                     /locus_tag="KRL67_004131"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HBH1524896.1"
                     /translation="MAKFKPLVDSSVKQAEAQVFDYWKDINILEKTLEKGKDDPSFVF
                     YEGPPTANGNPGVHHVLSRTLKDSVCRYKTMSGYQVKRKAGWDTHGLPVEIQVEKELG
                     LTSKQQIEEYGIAEFNQKCRESVFSFEKQWRIMTERMAYEVDLDNPYITLDNNYIESV
                     WWILNKFNKEGYIYEGHKILPYCPRCGTGLASHEVAQGYKEVKTNTVIAKFKKKDADE
                     YFLAWTTTPWTLPSNVSLTVNADVDYLKVKKGDEVYYVSKPLANKVLGEDYEVLEEMK
                     GKDLEGLEYEQLMPFVEVDKKAFFITVGDYVTTEDGTGIVHTAPAFGEDDYNTGKRYD
                     LPVIQPVDETGKFTSTPWEGRFVMEDGLDVEIIKWLASENKLYSKEKVVHNYPHCWRC
                     QTPLVYYAKPSWYIEMTKLKDKLIENNNGVKWFPSFVGEKRFGNWLENLNDWAISRTR
                     YWGTPLPIWRCECGHTDSVGSRAELAEKAIEDVNPETVELHRPYVDDIHLKCEKCGKP
                     MTRVTEVIDCWFDSGAMPFAQHHYPFENKENFDQLFPADYISEGIDQTRGWFYSLLAV
                     STFVLGKAPYKSVLVPDLVLDKDGKKMSKSRGNTVNPMELFDQYGADALRWYLLYVSP
                     PWTPTKFDMDGLKEIQSKFIGTMKNVYNFFTLYANTDDINPTEFFVEYKDRPELDRWI
                     LSKFNNLMKDVEENLAIFELNKTLRMIQDFINDDLSNWYIRRSRRRFWATELTEDKKA
                     VYNTTYELLHGLCRAIAPFAPYMSEEMYRNLTGEVSVHLAEYPKCNEELVDTKLEEKM
                     DLAKNLVTLGRASREVERIKVRQPLQKVLVDGKFEDTISDVVDLIKEELNVKEVIFAK
                     DLDEYMNFSLKPNFKEAGPLLGSKMNLFVGALSKLNAHETANKLEKGETLTVDLDGEA
                     FEFNKDLVLIGITAKEGFNVSVENNLFVILDTKLTEELINEGYAREFISKVQQLRKSN
                     NFEVLDNIVIEYCGDDEIAKAVDHFDEYIKSETLALEINRVDDKSLEEQNLNDHMTGI
                     KVIKK"
     gene            11192..12064
                     /locus_tag="KRL67_004132"
     CDS             11192..12064
                     /locus_tag="KRL67_004132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HBH1524897.1"
                     /translation="MYGESKKLSIDNNIFLQYCLIILGMILSTLGINLYLAPAKLLSG
                     GVAGICVILYKLFGINQGISSFLMNIPIFIIARKYFDNKFLLISFVNMLLFSLALGLT
                     QDIAKYFPIDDTMLQCIYGGALTGIGMGLTFKARATAGGLDIIAAIMKRKYDIPMKNT
                     FLFINFFVVCAGAFLFGAKLVMYTLITMYIISFTMDIGKDCFDRKKSILLISNKYDEI
                     SKVIMNKMGRGVTFLEAEGAYTQNKKKMIYCIISANEIGKFKDLIYDMDSEAFISVNN
                     VEEVKGGGFKDKFL"
     gene            12178..12450
                     /locus_tag="KRL67_004133"
     CDS             12178..12450
                     /locus_tag="KRL67_004133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH1524898.1"
                     /translation="MLRERFVVRNHHNPHTSGCCGTGGEGCCGGDNHEHSHQHSHSHG
                     HEHEHVNGVGHDHNHDDGFSGTHEHSHEHTHTHGHEHSDGHTHEHK"
     gene            complement(12613..13185)
                     /locus_tag="KRL67_004134"
     CDS             complement(12613..13185)
                     /locus_tag="KRL67_004134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBH1524899.1"
                     /translation="MKTNNMKQAILSSATNLIAKNGVQNTSLADIAKDVNISKGTLYY
                     HYASKNDIIYDIADTHLEVITSAILNCVKNVKSKNSQIEMVNLILEKISTIESRGRVH
                     MYLICEAITSNNDLKERMRLKYIEWRTTLKSEIVESLEKYNSSENEQDAESFSFLLMS
                     IVDGLVVQSLLKTEKIPYENIASFLVNHWI"
     gene            13369..14211
                     /locus_tag="KRL67_004135"
     CDS             13369..14211
                     /locus_tag="KRL67_004135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HBH1524900.1"
                     /translation="MDKIKLIVDSACDLPDDIIEKYNIEVVGLNVSFGEESYISGKEI
                     DNETFYRKMSESKVLPKTSCPSPDKFLESYHCQEESVLVINISSGLSGTYNSALLAKD
                     MFEKEGNNKKVEVIDSLSGSIGIGQLVLKAAILIKEGKEIEEIVSIINKYKDNPPFFG
                     TLETLENAIKGGRINPIAGKIINTLNFKAIIQVADGVVTPIDKARGEGNSLKKLITLV
                     ESKIKDKEEKVLFIGHANCPEKAQKVREVMEKDVKYKDVVICEVGSVMGTYTSKGAIL
                     ITAI"
     gene            complement(14314..15390)
                     /locus_tag="KRL67_004136"
     CDS             complement(14314..15390)
                     /locus_tag="KRL67_004136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HBH1524901.1"
                     /translation="MKAQRLNAVLEQMKKDDISQMLVSDPTSIFYLTGVLIHPGERLL
                     ALYLNLNGNNKLFINELFPVSEDLGVEMVWFNDTQNPVEIITEHIDKNATMGVDKNWP
                     ARFLLNLIELGGGSKFVNSSYIIDTLRMCKDEEEKELMRIASKLNDKAMEQLKATVSG
                     ELTEKQLVGKLSKIYEDLGTDGFSFDPIIGFGPNGANPHGEPGNALVKPGDAIILDIG
                     CIKDNYCADMTRTVFYKEIPEKGREIFEIVLEANKRAEAIVKPGVRFCDIDAAARDYI
                     TEKGYGQYFTHRTGHSIGLEVHDKGDVSSINTDTVQPGMIFSIEPGIYLPGEFGVRIE
                     DLVLVTEDGCEILNKHDKEICVVG"
BASE COUNT         6127 a         1823 c         2704 g         4945 t
ORIGIN      
        1 atttagaaaa tcacgagatt gctaagtaat acctttcatt attatgaaaa ctgtatttat
       61 tgttgagaaa acatatataa tatatgaatt ttctaaatag tataaaaata atagaatatt
      121 gaaaaaaaat tgacaaatga atgtataggt attaaaatat attaaatata tacataaaaa
      181 gtggtttact aacaaataaa tgaaatatat gttaatacat agttcaattt gtaaaaatat
      241 aagatttata aaaaaataag ggaggatatt attatgaaag caattttaac agtaattgga
      301 aaggataaac caggtatagt agcaggaatt agtgatgaat tatacaagca aaatatcaac
      361 atagtagata tatcacaaaa gatattacag gattatttta ctatgattat gatagttgat
      421 ttagagaaat cattaaattc ttttgagaaa acggttgagg acttggttga aagaggtaag
      481 aaattatcag tagatgtaaa aattcagcga gaagaaatat ttaattcaat gcataatctg
      541 tagtagacag taaggggagg tgataatagt gaatgtagga aatatacttg aaacaataaa
      601 gatgatagat gaagaacagt tggatataag aactataact atggggattt ctttattaga
      661 ttgtatagac cctgatggag ataaagctag agaaaaaata tacaataaga ttatgagttc
      721 tgctaaagat ttggtgaagg ttggaaaaga tatagagcgt gaatttggta tcccaattgt
      781 aaataaaagg gtatcagtaa ctccaatttc cattattgca ggtgcaacag atgaaaatga
      841 ctatgtaaag tttgcccaga cattggataa ggctgcagag gatttaggta tagattttat
      901 tggtggattc tcagcattag ttcaaaaggg atatacaaaa ggtgataaga tactaattaa
      961 gtcaattcca aaagctctag caagtactaa taaagtatgt gcttcagtaa atgtaggttc
     1021 tacaagatgt ggtataaata tggatgcagt gaaagaaatg ggagaaatca taaaagaaac
     1081 tgccgaatta acaaaagatg caaaaggttt tggttgtgca aaattagtag tattttgtaa
     1141 tgcagtagaa gataatccat ttatggcagg agctttccat ggtgttggtg aggctgatag
     1201 aattataaat gttggtgtaa gtggacctgg tgttgttaag agagctttag agaaagtaaa
     1261 gggagagccc tttgatgttg tttcagaaac agttaaaaag actgcattta aaattacaag
     1321 agttggacaa cttgtggcga aggaagcttc tagcagattg gacgttccat ttgggataat
     1381 agacttatca ctagctccta cacctgctat tggtgattca gtagcaaata tattagagga
     1441 aatgggtctt gaagttgttg gtactcatgg gactacagca gcattagcac ttttaaatga
     1501 tgctgttaaa aaaggtggag taatggcatg ttcacatgtt ggaggactta gtggagcgtt
     1561 tataccagta tctgaagatg caggtatgat tgatgctgtt attaaaggtg ctttaagtat
     1621 agataagctt gaagcaatga ctgctatttg ttctgtaggt cttgacatga tagctgtgcc
     1681 aggaaatact acagcaggta cgcttggagc tatgatagct gatgaggcgg caataggtat
     1741 gattaataat aagactacag ccgttagaat tataccagcc ccaggatgtg atgtaggtga
     1801 tatggtagaa tttggaggac ttttaggacg agcaccagta atgcctataa atacaaattc
     1861 atctgaatta tttactcaaa gaggaggaag gattccagct cctatacatt catttaaaaa
     1921 ttagttatat tacatataat aatatttaac taaatattta gatggttaaa ttaaaaaagt
     1981 ctatgcattt aaatattata gttaagataa taaactttgt taagataata aactttaaaa
     2041 atcaattgtt aagattgtgg aaaaaataaa gcgatacatg gacatttaaa aattcatgta
     2101 tcgcttttat tttaataata tctaattaat ataaaatttt ttatgtatct aaaaagttat
     2161 gctacctaac tagtaataaa ctatattaat ttttattcct aatatatcaa ccaaataaaa
     2221 aactaaacaa aaagctaaat aaaaataata aataaaaatt tatctaaata ttcaacacac
     2281 ataataatca taaacataaa taaaaaataa aacgtttgca attaataaaa ttgcctacaa
     2341 tataaaactc tactacttga gatataaatt tataaatctt attgtttttt aagcaatttc
     2401 ttcctctaca tcagtaccac gtatttcacc agcagtaaga actttaaagg tactgaatat
     2461 ttttgaacct aaaaatcctg caattacatt gaataaagca cataaaccag ctgttataag
     2521 aacttgttga actggattat aagcaaacat tatagcaaaa ccagccacag gtgtagctgt
     2581 acctggtgtc atgtttacaa gacccatcaa ggctacaatg ataccagaaa aggctcctcc
     2641 aaagaaatta gtagaatata taggtattgg attagctgaa attatgtctg attgggttaa
     2701 tggttcaata gcaacagata taatatcttt tttagaacct aatttcaatt ttgcaaatag
     2761 cgttgagttc ataaaagctg aaccaaaagc acctaatgct cctattgcca taggaagacc
     2821 agtaagacca attatagatg tgagagccat tgaactaagt ggagacgttc caactactgt
     2881 tattagacct cctagtataa ttcccattat tatagggcta atgttggtag cttctatcaa
     2941 aacttgtcca atttgtagta gtgtactatt aactaatggg tctacatttg atgctataag
     3001 acgagatagt ggtgcacata gaagaattac aaccactaaa tccactccag gtggcacttt
     3061 tttctctata aacttaatta agaaagaaac aaagtatcca gcaacgaatc cagataatat
     3121 agaaaaatct aaacaagcta atcctattaa aactgcataa actggagata cacctaaggc
     3181 taaaggcaca agtatagcag cagcaactcc accaagacta ccattagcag ctccaacact
     3241 tctaaaaaat tccataccta aaacatcacc aagtaaagaa aggttgaatg cctctactaa
     3301 gaaagtagcg catgcagcgt ttgctagagc tcccatagcc ttcatgccat agggcgcctt
     3361 gtaactaaaa attgtgaaac aacatagaac caataaaagt aaacctgttc ctaataaaat
     3421 ttctgacata agaaaatcct ccttgtattt tttttaggac ataaactttt gccatgataa
     3481 cgttatcctg attcgtaaat aattacctaa ataaatgtca acagaacaaa agttatttta
     3541 aatcttaatt ggttttatat tgcatctaaa aataaaaata actataaaaa catataaagt
     3601 aagcttaacg tataaaatat aaattaaact tatactttat aatacaatta tatataaata
     3661 atgcagtctt gtataggaag attgataaaa aataaattta atatggaaaa aataattatt
     3721 agaacgtaaa atattagctt ataaaaaagg aataaacatt ttttagaaga agtacttata
     3781 atgaaagtaa ttttataggg ggagagtctt tgaaaaaggt aaaatatacc agattagtga
     3841 tacttggtat tcttatatat ttagtattta atatattagt aatgataatt agcaaaaatg
     3901 tagatacgtt ggtgttaaaa aatgaaaaag ttgatgcaaa gattcatgta aaaggaacta
     3961 tagaaaaaga tgccaatgac caaagtaatt atctatctac atacatcagt aaagaagatt
     4021 ctaataactt tgaaaaaaat caaaatgtaa taattgaata tcatgagcaa aaattagatg
     4081 caaaagttta caaaatatat gaaaataata ataaaataat ggtaaaatta aaaataagta
     4141 accaaattgt tgcaaatcag gatacaagtg tcgaagaatt tgatataata tataatcaga
     4201 tggaatgttt aaaaatacct aaaacttcta taaaaacaaa agacaataaa cgtggtgtat
     4261 atgtaattga tgaacaatca caaagtgtaa aatttgtcat attagaagga ataacttatg
     4321 agaatgaatc ttctgtattt gtggattatt ataaaaatga cataaatgga gttacaagtg
     4381 ttaatctata tgacaaaata attttaagac ctaacagtat aaacacaaat ataaggataa
     4441 agtaggtgat aataatgggt tctattaaag ataatttgtc tgagataaga aacaaaattg
     4501 ataaagctgc aaaaaaagta gatagagaca gcaaagagat tacccttttg gcagtcacaa
     4561 agactgttga tgtagactta gttaatgagg caatgaggga aggaatcact tctgttggtg
     4621 aaaataaacc ccaagaatta gctagaaagt atgatattat aggtgacaaa ttaaactggc
     4681 acctgatagg aactttgcaa accaataaag ttaagtacat aatcgataaa gtttgtatga
     4741 tacactcttt agatagaatt gcattatgcg aagaaattca aaaacgtgca gaacaaataa
     4801 acagagaaat agattgctta gtacaagtga atatatctaa agaagaatct aagcatggac
     4861 ttgatagaga agaagttgta gactttgtca aagaagtgtc agaaaaatat aaaaatataa
     4921 aaataaaggg tctaatgaca atggcacctt ttatagaaga tgaagatgag ataagaggtg
     4981 tatttaaagg tcttaaagat ttatcaaaac aaataaaaga actaaacatc cctaatgtag
     5041 ggatggatgt tttgtcaatg ggaatgagtc acgattatga aattgctata gaggaagggg
     5101 ctaccattgt tagagttgga acatcaatct ttggagaaag aaactacaat aaaaaataat
     5161 tactcaaatt tgaataaatt ttaaggagga agttaataat gtcaaatgga ataatatcta
     5221 aatttaagaa ttggatagtt gatgaagaag aagattacat tgaagatgaa tatgaaagtg
     5281 gcatggacga tatagttcaa gaagaagaaa tgaacagtgg ctttagtact gcaaaagcaa
     5341 ataaaattgt aaatttacat acaactagcc aaatgaaagt tgtaatagtt gaacctaagg
     5401 tttatgatga agctgcaact atagcagacc atctaaaaca aagaagggca gttatagtga
     5461 atttggaagg attgactaat tctgaagttc gtaaatctat atttaacttt atgaatgggg
     5521 cagtttatgt cttagatgga agtatacaaa aggtttctaa atcaatattt atactagctc
     5581 ctaataatgt ggatatagat gcaaatatga aaaaagaatt agaaagtaaa gcattcttcc
     5641 cttggcaaaa taaatagtaa aaaggcggtt aatatatggg aacaataaga attgcacttt
     5701 attatctttt agatataata gcatggatga tagttataaa aagcttgatg acttggtttc
     5761 caaatggaag acaaagtaaa atttttgata ttttggaaaa tcttacagag ccaatagaag
     5821 ggccaataag gtctataatg tataaatata caaatggacc tatagatttt tctcctttaa
     5881 ttgcaatagt attattgatg ttcctaagac aattagtatt ggttatattc taaggtgata
     5941 ggatggataa attaaaacta acaaatcaca taaaggatat agacttgaaa aataaaatgt
     6001 ttaaagtgat agacaaggct aatagttgta taaaaaacta tgatgttaag tctacagagt
     6061 ttcttaatcc atatgaagtg aaaaatgctg tagcaatatt aaattctaca aatgaaataa
     6121 aatacagtgt ggatggaggt tatgaacaag ctgaaagaag cactgtattt atttatccat
     6181 tttacatgga atacgaagat atagaagata ctcttagatt tttacaaata gaagggaatt
     6241 tcaaatttaa aaatatatct cataaagatt acttagggtc aatactgagt ctaggtataa
     6301 aaagagaaaa aattggagat ataattatac atgatagctt ttgtcaagta gttgtaagta
     6361 gtgatatttg cgattttata attgtaaact tagaaaaggt ttcaagaaat aatgtaattg
     6421 taaaggaaat ttccagagaa aatattgtta atagttcttc aaaatataaa gaagtatcat
     6481 ttacagtatc ttcagatagg ttagattgtg tgataagtgg tatttacaat atatctagac
     6541 aagatagtgc aaaatacata aatggagaaa aagtccatgt caattatgaa aagataactt
     6601 caacttctaa aatagtaaaa aatgatgatt tgatttctat tagaggcaaa ggaagagcta
     6661 aggtaacaca aataggagat attactaaaa aaggaaaaat aaaagttcaa gcaagattaa
     6721 ttgtataagg actaatttaa attatagctg ggaggaaatg taatgctaac tccaattgag
     6781 atagaaaata aagagtttaa aaaaggttta agagggtata gagatgaaga ggttgatgaa
     6841 tttttagata tcatcaagga agattatgaa tctctatgta gggaaaatac agctcttaaa
     6901 gaaaaattag ggttatatca agaccaagta aacaagtatg aaaatattga agaaacacta
     6961 aaagctactt taataactgc tcaaagtgca gcagaagaca cttgtagcgc ggctaataaa
     7021 aaggctaaga taattgttga agaagctgat ttaaaagcta gacaaattat agaacaagct
     7081 aataaccgtg ttatagaaat aagaaaagag tatgatgctt tagtcaaaga atttaagata
     7141 tttagaaata aatttaaatc actgttggaa gatgaaataa gaagtgttga tgagattttt
     7201 tatgatgtag atgaaaaatg tgtaaatgca tttgaaggaa caacagtgta taattatagt
     7261 aatgatgaag ctgctacaac tttagaataa gcttgtaatt tgtttatttt ttatgtagaa
     7321 tcattgtgta agtttgagaa atctataaca aagattaaaa ataggataaa aatgtaagaa
     7381 aaatagaaat tattatattt ttcaaaaata tattgactat tcaaattatt tgttatagaa
     7441 tttcatataa acacagataa atttttaaaa gcgttgatag agacatttat ctttagataa
     7501 ttttacagag aattgggtat gctgagaacc aatgaattta taaagataaa aatcactctt
     7561 gagctgcagg ctgaacgatt aaattctact aagctgtgcc ggttagtacc gttatactat
     7621 tagagtgtta tatgtagcta gtatttagtt tacatataga attagggtgg taacgcgatt
     7681 tataactcgt ccctttggtt taaagggggc gagtttttta gtatttaaat ttatgtgaaa
     7741 gaataaaaaa ttttggaatt ataatattta taggaaaggg tgatttacat ggcgaaattt
     7801 aagccattgg tagacagttc tgtaaaacaa gcagaagcac aagtttttga ttattggaaa
     7861 gacataaata tacttgaaaa aactttagaa aaagggaaag atgacccaag ttttgtattc
     7921 tatgaaggac ctccaacagc taatggaaat cctggagtac accatgtttt atctagaaca
     7981 cttaaagatt cagtttgtag atataaaaca atgtctggtt atcaagtaaa gagaaaagca
     8041 ggttgggata cacatggatt accagttgaa atacaagtag agaaagagtt aggtcttact
     8101 agcaagcaac aaatagaaga gtatggaata gctgaattta accaaaaatg tagagagtca
     8161 gttttctcat ttgaaaaaca atggagaata atgactgaaa gaatggctta tgaagttgat
     8221 ttagataatc catatataac tttagataat aactatatag agtctgtctg gtggatactg
     8281 aataaattca acaaagaagg atatatctat gaagggcata aaatacttcc ttattgccca
     8341 agatgtggaa ctggtcttgc tagtcatgag gttgcacaag gatataaaga agtaaaaact
     8401 aatacagtta tagctaaatt taagaaaaaa gatgctgatg aatacttctt agcatggaca
     8461 acaactccat ggactctacc atcaaatgtt tcgttaacag taaatgctga tgtagattat
     8521 ttaaaagtta aaaaaggtga tgaggtatat tatgtatcta aacctttagc taataaagta
     8581 ttaggtgaag actatgaagt tttagaagaa atgaaaggta aagacctaga aggtttagaa
     8641 tatgaacaat taatgccttt tgtagaagtt gacaaaaaag cattttttat tacagtagga
     8701 gattatgtaa ctactgaaga tggtactggt atagttcata cagcaccagc atttggtgaa
     8761 gatgactaca acacaggtaa aagatatgat ttaccagtta ttcaaccagt agatgaaact
     8821 ggtaaattta catctactcc ttgggaaggt agatttgtaa tggaagatgg gctagatgtt
     8881 gaaataataa aatggctagc ttctgaaaat aaattatata gcaaagaaaa agtagttcat
     8941 aattatccac actgttggag atgtcaaact ccactagtat actatgcaaa accaagctgg
     9001 tatatagaga tgactaaact taaagataaa ttaatagaaa ataataatgg tgtaaaatgg
     9061 ttcccaagtt ttgtaggaga aaagagattt ggaaactggt tagaaaatct aaatgactgg
     9121 gcaatatcaa gaactagata ttggggaact ccacttccta tatggagatg tgaatgtggt
     9181 catacagatt cagttggttc aagagctgaa ttagctgaaa aagctataga agatgtaaat
     9241 ccagaaacag ttgaacttca tagaccatat gtagatgata tacacttaaa atgtgaaaag
     9301 tgtggaaaac caatgacaag agtaacagag gttatagact gttggtttga tagtggggct
     9361 atgccatttg ctcaacacca ctatccattt gaaaataagg aaaactttga ccaactgttc
     9421 ccagctgatt atatatcaga aggtatagac cagactagag gttggttcta ctcattactt
     9481 gcagtatcga catttgtatt aggaaaagca ccatataaga gtgttttagt tccagacctt
     9541 gtacttgata aagatggtaa gaaaatgagt aaatcaagag gaaacacagt aaatccaatg
     9601 gaattatttg accaatatgg agcagatgca ttaagatggt atttactata tgtttctcca
     9661 ccatggactc caactaaatt tgatatggat ggattaaaag aaatccaaag taaatttata
     9721 ggtactatga agaatgtata taatttcttt actttatatg caaacacaga tgatataaat
     9781 ccaactgaat tttttgtaga atataaagat agaccagaac ttgatagatg gatattatct
     9841 aaattcaata atttaatgaa agatgtagaa gaaaatttag ctatatttga gttaaataaa
     9901 actttaagaa tgatacaaga ttttataaat gatgatttat ctaactggta tataagacgt
     9961 tcaagaagaa gattctgggc aactgaatta acagaagata aaaaagcagt gtacaataca
    10021 acttatgaat tgttacatgg tctttgtaga gctatagctc catttgcacc atatatgtca
    10081 gaagaaatgt atagaaactt aacaggagaa gtatctgttc atttagctga atatcctaag
    10141 tgtaatgaag agttagtaga tactaaatta gaagaaaaaa tggatttagc taaaaactta
    10201 gttactttag gtagagcttc aagagaagta gagagaataa aagttcgtca accattacaa
    10261 aaagtgttag tagatggtaa gtttgaagat acaataagtg atgtagttga tttaataaaa
    10321 gaagagctta atgttaaaga ggtaatattt gctaaggatt tagatgagta tatgaacttt
    10381 agtttaaaac caaactttaa ggaagcagga ccattattag gttctaagat gaatttattc
    10441 gttggagcat taagtaaatt aaatgctcat gaaactgcta ataaacttga aaaaggtgag
    10501 actttaacag ttgatttaga tggtgaagct tttgaattta ataaggattt agtattaata
    10561 ggtataactg ctaaggaagg atttaatgta tctgttgaaa ataatctttt tgttatacta
    10621 gatactaagc taactgaaga gttgattaat gaaggttatg ctagagaatt tatatctaaa
    10681 gtacagcaat taagaaaatc taataacttt gaagtattag ataatatagt aatagaatac
    10741 tgtggtgatg atgaaattgc taaggctgtt gaccattttg atgaatacat caagtctgaa
    10801 actttagctt tagaaataaa cagagtagat gataaatctt tagaggaaca aaacttaaat
    10861 gaccatatga caggtataaa agttataaaa aaataaaaag aatagttagt taaccaaaat
    10921 ccctatagaa attagtttat agatttctat agggattttt tatgtaatat aattatctat
    10981 tcaataaaat gttttaatat tccaaaattt acaataaata aatctatata aatcatataa
    11041 attatttatg tcatgtgata gaatattaat gaataaacgt ttaattgagg atgaataata
    11101 tttttagtaa gctacttctg ataaatttat ttataaattt atcgtgtgtt ctgcagggtg
    11161 gtttatttat tatgcaaagg ggtgcgtttt gatgtatggt gaaagtaaaa agctaagtat
    11221 tgataataac atatttttac aatattgtct tattatattg ggtatgattc tatcaacctt
    11281 aggaataaat ttatatctag caccagccaa gttattaagt ggaggagtag ctggaatttg
    11341 tgttatatta tacaaattat ttggtataaa tcagggaata tcaagtttct tgatgaacat
    11401 accaatattt attatagcta gaaagtattt tgataataaa tttttgctaa taagttttgt
    11461 caatatgtta ctattttctc tagcactggg gctaacacaa gatattgcca aatattttcc
    11521 aatagatgat actatgctac agtgtattta tggtggtgca ttaactggta taggtatggg
    11581 gttaactttt aaagcaagag ctactgctgg aggacttgat ataatagcag ccataatgaa
    11641 aagaaagtat gatataccta tgaaaaatac atttttattt attaattttt ttgtagtatg
    11701 tgcaggagct ttcttatttg gagctaaatt ggtgatgtac actttaatta caatgtatat
    11761 aatttctttt actatggata taggaaaaga ttgctttgat agaaaaaaat ccatactttt
    11821 aatatcaaat aaatatgatg aaatatccaa agtgataatg aataaaatgg gaagaggagt
    11881 cacttttttg gaggcagaag gtgcatatac acaaaataag aaaaaaatga tttattgtat
    11941 aatatctgca aacgaaatag gtaaatttaa agatttaatt tacgatatgg atagtgaagc
    12001 ttttatttct gtcaataacg ttgaagaagt aaaaggtgga ggctttaagg ataaatttct
    12061 ttaagattgt atatgttaaa aaataccata tgtattgaga ttatacctag ttagatgtat
    12121 actgtagtta atggggtaca tgaagtactc aataatatac taggaggtat aagaattatg
    12181 ttaagagaga gatttgtagt aagaaatcat cataatccac atacaagtgg atgttgtggg
    12241 actggaggag aaggatgttg tggaggagat aaccatgaac attcacatca acattctcac
    12301 agtcatgggc atgaacatga gcatgtaaat ggtgtaggtc acgaccataa tcatgatgat
    12361 ggtttttcag gtactcatga acatagccat gagcatacac atacacatgg tcatgaacat
    12421 tcagatgggc atacacatga acataaataa aaacatttta aatttataag agatttttat
    12481 tttgtgattc catgaaggtt taaataaaaa ttgaatattt taagtaaaaa ttattgacaa
    12541 taaataagaa agtgaactgt taaagtagat aacttaaata gttcactttt attaattatc
    12601 taagtattaa atttaaatcc aatgattaac taaaaaagaa gctatatttt cataaggtat
    12661 tttttctgtt tttaataaac tttggacaac taaaccatca actattgaca tgagtaaaaa
    12721 agaaaatgat tcagcatcct gttcattttc gctactatta tatttttcta gtgattcaac
    12781 aatttcagat ttaagagtag ttctccattc aatatatttt aatctcattc tttcttttaa
    12841 atcattattt gaagtaattg cctcacaaat aagatacata tgtaccctac cacgactttc
    12901 tatagtagat attttctcta agattaagtt aaccatttct atttgagaat tttttgattt
    12961 gacatttttt acacagttta gtatagcact agttataact tctaaatgag tatccgcaat
    13021 atcatatata atatcatttt tagatgcgta gtgataatac aaagtacctt tacttatatt
    13081 tacatctttt gcaatatcag caaggctagt attttgaact ccatttttgg ctattaaatt
    13141 ggttgcagat gataaaatag cctgtttcat attatttgtt ttcataaaac ctacctttcc
    13201 atgtatattt ataacaaatt aataacatta taagcttatg aatatttatt gtcaataaag
    13261 cgagaatcta tatctattga ctttttttca ttcaatatat aaaataataa ttaaatcggt
    13321 cgtacgaccg actgaataaa taaaataata tcttatagtg aggttaatat ggataaaata
    13381 aaattgatag tggacagtgc atgtgactta cctgatgata taatagaaaa atacaacata
    13441 gaagttgttg gtttaaacgt atcttttgga gaagaaagtt atatatctgg aaaggaaata
    13501 gataatgaaa ctttctatag aaaaatgagt gagagtaaag tgctaccaaa aacatcatgt
    13561 ccttctcctg ataaattttt agaatcatac cattgtcagg aagaaagtgt attggtgatt
    13621 aatataagtt caggattatc tgggacttat aatagtgcac ttttagcaaa agatatgttt
    13681 gaaaaggaag gaaataacaa gaaagtggag gttatagatt cacttagtgg gtcaattgga
    13741 ataggacaat tagtgttaaa agcagcaata ttaataaaag aaggaaaaga aattgaagag
    13801 attgtttcaa taataaataa atataaagat aatcctccat tttttggaac acttgaaact
    13861 ttagaaaatg ctattaaggg tggtagaata aatccaatag caggaaaaat tataaatacc
    13921 ttgaatttta aggcgataat acaagttgcg gatggcgttg taactcctat agataaggca
    13981 agaggcgaag gaaacagttt gaaaaagttg attactttgg tagagtcaaa aattaaagat
    14041 aaagaagaaa aggttttatt tataggacat gctaattgcc cagaaaaggc tcaaaaggtg
    14101 agggaagtta tggaaaagga tgtaaaatac aaagatgttg taatctgtga agttggttca
    14161 gtgatgggga catatacttc aaagggtgca atcttaataa ctgcaatata aataatgaat
    14221 tgaaataaat catagattga gaaattaaag ccatataaaa atgcttaaaa catttttata
    14281 tggtttttta attttatcaa attgatttta gaattaacca actacacata tttctttgtc
    14341 atgtttgttt aatatctcac aaccatcttc agtaacaagt actaaatctt ctattctaac
    14401 tccaaactca cctggtaaat atatacctgg ctctatagag aatatcattc ctggttgtac
    14461 agtatctgta tttatagaag atacatctcc tttatcatga acttcaagtc ctatagagtg
    14521 accagttcta tgagtgaagt actgtccata tcccttttca gttatgtagt cacgagcagc
    14581 agcatctata tcacagaatc taacacctgg ttttacaata gcttcagctc tcttattagc
    14641 ctcaagaact atttcaaata tttctctacc tttttcaggt atttctttat agaatacagt
    14701 tcttgtcata tcagcacaat agttatcttt tatacaacct atatctaata ttattgcatc
    14761 tcctggtttc acaagtgcat ttccaggttc accatgtgga ttagctccat taggtccaaa
    14821 acctataata gggtcaaatg aaaatccatc agtacctagg tcttcatata ttttggataa
    14881 tttaccaaca agttgtttct ctgtaagttc accagaaaca gtagctttaa gttgttccat
    14941 agccttgtca tttaattttg aagctattct cattaactct ttttcttctt catctttaca
    15001 cattcttaaa gtatctatta tataagatga atttacaaac ttacttcctc cacctaattc
    15061 tattaagttt aataagaatc tagctggcca gtttttatca acacccatag tagcattttt
    15121 gtctatatgt tcagttatta tttctactgg attttgagta tcattaaacc aaaccatttc
    15181 aacacctaaa tcttctgaaa ctgggaataa ttcatttata aataatttat tattgccatt
    15241 taaatttaag tataatgcta ataatctttc acctggatgg attagtactc ctgtaagata
    15301 aaatatagaa gtagggtcac ttactaacat ttgagaaata tcatcttttt tcatttgttc
    15361 aagtacagca tttaatcttt gagctttcat aataatttcc tcctaatcaa aattcaatac
    15421 aaatcaattt tagcattatt aaacaaaaaa gagtatactt ttcttctaga aatataaaaa
    15481 aaatttgtta tatatttttt atattaagtt taatctctac ttttgcatta aatacggaag
    15541 taaaatgact ttttattgtg aaaattctat aaaagtaata aattaagtct ataaagtta
//
LOCUS       DAEPXF010000426        13380 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain HB27
            SAMN13376389-rid12479423.guided.104, whole genome shotgun sequence.
ACCESSION   DAEPXF010000426 DAEPXF010000000
VERSION     DAEPXF010000426.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13376389
            Sequence Read Archive: SRR10518432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 13380)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13380)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001069624.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001069624.1
            Genome Coverage       :: 315x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/15/2021 01:54:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,165
            CDSs (total)                      :: 4,076
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 89
            rRNAs                             :: 10, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 10 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 19 of 69
            Pseudo Genes (incomplete)         :: 44 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 10 of 69
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13380
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13376389-rid12479423.guided.104"
                     /strain="HB27"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="China: Hebei"
                     /collection_date="2017"
     gene            complement(147..881)
                     /locus_tag="KRL67_004137"
     CDS             complement(147..881)
                     /locus_tag="KRL67_004137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine biosynthesis protein TtcA"
                     /protein_id="HBH1524902.1"
                     /translation="MSIQKLLSKARKAIQDFDMIQENDKIAVGLSGGKDSLTLLHILK
                     NYQRFSPQNFELIAITLNTGGVDNSPLDKLCKEINVPFYEFQTDIKEIVFDIRQEKNP
                     CALCANLRRGALNDNAKKLGCNKVALGHHKDDAIETFLMSMFYEGRVNCFSPKTYLDR
                     QDLTVIRPMIYIEEYMTKKISKDSNYPIITNPCPANGHTRREYIKNLIANLNKEMPDF
                     KRNVFGALNNSEKLFIWDKEKIKNFK"
     gene            1007..1723
                     /locus_tag="KRL67_004138"
     CDS             1007..1723
                     /locus_tag="KRL67_004138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HBH1524903.1"
                     /translation="MKKFKLIGAIILVVFISVFISKERLLKKDKDPIVVAQHDSEQDV
                     EANSQNKNHSNSISNILLVNKTNGISKNYTPENITKVNIPFVEEATEEEKQMAGEPAK
                     AVEDLVKQANSEGIQFLGSSAYRSYDTQLDTYTRRVKSQGREKADAYVAKPGYSEHQT
                     GLCIDLTNPERWFVGSTKEAKWLAENAHKFGFIIRYPEGKEDITGTAYEPWHIRYVGK
                     DAAEEIYSKGLTLEEYLQNK"
     gene            1857..2549
                     /locus_tag="KRL67_004139"
     CDS             1857..2549
                     /locus_tag="KRL67_004139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011948339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBH1524904.1"
                     /translation="MNNTVLVVDDEKEIRDLIEIYLLNSGYNVIKAEDGKEALDILKN
                     ENIHLMVLDIMMPKMDGIEVCRKVRESLNIPILMLSAKSEDMDKIQGIMTGADDYLTK
                     PFNPLELTVRVKSLIRRAYYFNGSNNEDKDVIEVGVVIIDKEKHSVTVAGNEIILTSR
                     EFDILYLLANNKGRVYSTEEIFEKVWKEKYYQSNNTVMVHMSRIRDKIEKYTDGEKII
                     HTVWGVGYKIEK"
     gene            2539..3720
                     /locus_tag="KRL67_004140"
     CDS             2539..3720
                     /locus_tag="KRL67_004140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HBH1524905.1"
                     /translation="MKNKSLLNHMFRPILDLIISCGLTSMLILTSMYIIKLLYFNSEI
                     SVINQLVYQLRDFRSLIGESLFYPFLFTITSLAIYSMISYKRNKQIVDFIRKTNRKNQ
                     KLEIIKSAVNLMDEGNLEKSIDIEYDLDILKDKEKDDIDELAHKINNIVHQLRNITIE
                     ERQAQQTKTDLITNVSHDLRTPLTSIMGYLGVIEEGKYKDEVQMMYYVDIAYEKSKNL
                     NVLINDLFELTKMQNNTIKLNKIEINLVELLSQVVSQFEIYFKQEFMVSRINFNEEKL
                     IVKADPNKLVRAFENLITNAVKYGKDGHYVDIVTEKKEDMAVVKVINYGEPIPVLDLP
                     NIFDRFYRVEKSRNRNVGGSGLGLAITKNIVNLHNGEITVSSDKYQTVFEVQLPIIQS
                     A"
     gene            4002..4457
                     /locus_tag="KRL67_004141"
     CDS             4002..4457
                     /locus_tag="KRL67_004141"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HBH1524906.1"
                     /translation="MEKVESFKLDHTKVKAPFVRKCSVLDGVKGDKVTKFDLRFLQPN
                     VESFGTAAMHGLEHLLATYLRDTLDGVIDLSPMGCRTGFYLILWGDVDAKTVKIGLEE
                     ALKKVLESDKMPAATAVECGNYRDLSLFGAKEYAKDVLDKGFSLNIYGE"
     gene            4447..5172
                     /locus_tag="KRL67_004142"
     CDS             4447..5172
                     /locus_tag="KRL67_004142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Vitamin B12 dependent methionine synthase,
                     activation domain protein"
                     /protein_id="HBH1524907.1"
                     /translation="MENNLIGREDISINQSEVLRYLQYRGQEIDKDLKYTIDECVAIT
                     KNKINPRYLSRIYPIRINKDNIVELEGTNLTLESKDIYELLKECNECVIMAATIGIDI
                     EKEIRRHSYSNLTKGLIIDSCGTTAIEEVCDIVQNNLEKVFLKQDKFITMRYSPGYGD
                     LPIEKNIDILNVLNAQKQIGLTITSNGIMIPRKSVVAIIGISNTRINKSKKEKSCENC
                     KNYRNCTYRKGANSCGNKTISEK"
     gene            5144..7525
                     /locus_tag="KRL67_004143"
     CDS             5144..7525
                     /locus_tag="KRL67_004143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine S-methyltransferase family protein"
                     /protein_id="HBH1524908.1"
                     /translation="MEIRQYLKNNILIFDGAMGTMLQQKGLKLGENPEVFGLQNPDKL
                     IEIHTAYLEAGSNVILTNTFGCNELKLDSKYTVEEVIDNAVLVARKAIENVDNTKPRY
                     VALDIGPIGEMLEPMGTLSFDKAYEIFKRQVLQGVKSGVDVIVIETMMDLYEAKVAVL
                     AAKENSDLPIFCTMTFDEGGRSFTGCMPECMVATIEGLGVDAIGVNCSLGPKQLLPIV
                     EKIASRATVPVMVQANAGLPNIVDGEAIYDVDAKEFFEGVKKFVEVGATIIGGCCGTN
                     PSFIKEISENINSVTKGCIEKIDECVVCSPSKFVEVQSPTVVGERLNPTGRKSLQEAL
                     KNENVDYAINLGLEQVNAGAQILGVNVGLPEIDEKKLMPKLIREIQAVVDTPLQVDSS
                     NVEALEQGLRYYNGRTIVNSVNGKEESLESILPIVKKYGSCVVGLTLDEKGIPKKAEE
                     RFEIAKRIVNRAVSYGIKPKDIFIDCLSLTVSAQQEEAIETIKAITMVKTLGVKTILG
                     VSNISFGLPNRKALNASFLTLALGAGLDLAIINPNEYSMMEAINSFKILNNTDKGCIN
                     YINQYSNINNSKKSDSSTKIDKDLPLDILVERGLKDEAKNITLNLLKEKDENYILDEI
                     LIPALDKVGKRYDSGDIFLPQLIQSAETVKVSLNTIKETLLSKSSNNVSKGKIIVATV
                     KGDIHDIGKNIVKIMLENYGYEVIDLGKDVPIEEVVEVAKKRNIKLVGLSALMTTTVQ
                     SMKDTIQALRDNEINAKVFVGGAVLTEEYAEEMGADYYSKDAKSAVEIAKLNF"
     gene            complement(7730..8959)
                     /locus_tag="KRL67_004144"
     CDS             complement(7730..8959)
                     /locus_tag="KRL67_004144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HBH1524909.1"
                     /translation="MNLNRNLEKYAELAIKVGVNIQPGQILLIKSPIECADFARNALK
                     EAYKCGAKNVYIEWSDEESTLIKYLYAPDEAFHEFPKWTAEQYVDIAKEGGAFLSVYA
                     QNPDLLKDVDPERVANFQKASGKALKEWRSYTLTDKCKWSIVSVPTKDWAKKVFPNLS
                     EDKAIEKLWDAIFKCSRVDGQDPIKAWEEHNENLKSKMDFLNKNNFKTLKYKSSKTDL
                     TLDLPKGHVWLSGASKDPNGISFNPNIPTEEIFGMPHKFKVNGTVYSTKPLVYGGNII
                     DNFFLTFKDGKIIDFSAEKGLDTLEKLIETDEGSHYLGEVALVPYNSPISNTDIIFYN
                     TLYDENASCHFAIGSAYKTCLEGGNNLKDEELDEHGVNDSLTHVDFMIGSADMDIIGE
                     TYDGKQIQIFKNGNWAF"
     gene            9559..10473
                     /locus_tag="KRL67_004145"
     CDS             9559..10473
                     /locus_tag="KRL67_004145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH1524910.1"
                     /translation="MELYEAIFYRKSVRNFSSKKIKKPLMEEIKRSCKNLEYLNEDLN
                     IKAHVVDRGHIIHFLMGKECKVKAPHYIVVTSDKGEDYLQNIGFATEGVVLRLTTLGI
                     ATCWLEGNLKREDILEFVDIGNKNFEEDEEDDLDYLAGIKTEEDDENKIENPYIIIAF
                     GYPAKNEDLFRTRYARPDRKPVKDMINKINRNRKWKDILELTRRAPSVKNSQPWYFHK
                     DERGLHLFEKRPKKHCEDMNKVSLGVALRHFDIACIKNKIDVSYEKLPIRNKIGKSYF
                     ITVVEHVKPEEEIQEENVILEKEESQDE"
     gene            10497..11129
                     /locus_tag="KRL67_004146"
     CDS             10497..11129
                     /locus_tag="KRL67_004146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1847 domain-containing protein"
                     /protein_id="HBH1524911.1"
                     /translation="MYKCGKCNLRGCSNGNMDKLMKICPTREIELQQKAKQLYEEEEN
                     RKIAYNAGLVEAEGYCQYTRLKEIIEFAKKCGYKKIGIAFCIGLKREMQTVQKIFEHH
                     GFEVFSVICKNGAMPKSTIGLDASQTISKCSDEVMCNPIGQALFLNEAKVDLNVILGL
                     CVGHDTLAMKYMEAPITTLVVKDRVTGHNPVAVIYNAESYFEKKLFDKEE"
     gene            11505..12221
                     /gene="pyrF"
                     /locus_tag="KRL67_004147"
     CDS             11505..12221
                     /gene="pyrF"
                     /locus_tag="KRL67_004147"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HBH1524912.1"
                     /translation="MINGKEKLIVAIDTDEFDKAKELIDRLEDSVDIFKVGLEQYVAT
                     KGKTIDYLKEKGKKIFLDLKFHDIPNTMKSAVRAAVRDNVWLMTIHVSDMEGMRQCAL
                     IAREEAERLNIEKPLVVGVTVLTSLSNQDLQDIGCNMTTEELAIKRAKLAKESGIDGV
                     VCSAQEVDKIVEACGTDFITVCPGIRPKSAEVGDQKRVVTPSDAIKKGAHYLVVGRPI
                     TKAENPRESAINIVREIENA"
     gene            12247..13290
                     /gene="carA"
                     /locus_tag="KRL67_004148"
     CDS             12247..13290
                     /gene="carA"
                     /locus_tag="KRL67_004148"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011862004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HBH1524913.1"
                     /translation="MKGKLILEDGTVFEGKAFGYLKETIGEVVFNTSMAGYGELLTDA
                     TYYGQIVTMTYPLIGNYGINLEDVESNKVQVKGLIVREKSDNPNNFRCEMDIDTYLKQ
                     NKVIGLEGIDTRALTKIIRNNGTKRGIITLENVSLQDVQSKLEEHCNKDSVKNVTRKE
                     IENIKGTGAKVVVIDFGVKNSVLESLKACNCDITIVPAMTTVEEILNINPDLILLSNG
                     PGNPEDINDIVENIKKLIGKKPLTGIGLGHQILALALGGKTSKLVFGHRGGNHPVKCL
                     ETGKVFITSQNHGYNVSEMPENTEITHINLNDNTIEGMRHKELPIYSVQYIPEYTTKE
                     DLDCIFNKFLALI"
BASE COUNT         5369 a         1470 c         2359 g         4182 t
ORIGIN      
        1 tagataaagt aaatactatt aagaactatt aaaatataga ttaaagataa taacatataa
       61 taaataaaaa agtaagattg tgtagattat ataaagatta tttacaatta atacaatctt
      121 gcttttttat ttaaaaattt taattattat ttaaaatttt ttatcttctc tttatcccat
      181 ataaacaatt tctcagaatt atttaaagct ccaaatacat ttcttttaaa atcaggcatc
      241 tctttattta aattagcaat aagatttttt atgtactctc tcctagtatg accatttgca
      301 gggcatggat ttgtgataat aggatagtta gaatcttttg aaattttctt agtcatatac
      361 tcttctatgt aaatcatagg tcttataact gttaaatcct gtctatctag gtatgtcttt
      421 ggggaaaaac aatttactct accctcataa aacatagaca ttaaaaatgt ctcaattgca
      481 tcatctttat gatgaccaag agctacttta ttacatccaa gttttttagc attatcgttc
      541 aaagcacctc ttcttaaatt agcacataat gcacaaggat tcttttcttg tcttatatca
      601 aatacaattt cttttatgtc agtttgaaat tcataaaaag gaacatttat ttctttacac
      661 aatttatcta gtggagaatt atcaacacct ccggtattta atgttatggc tatcaattca
      721 aagttttgtg gagaaaatct ttgataattt ttaagtatat gaagtagagt taaactatct
      781 tttccaccag aaagtccaac cgcaatttta tcattttctt gtatcatatc aaaatcttgt
      841 atagccttac gagctttact tagaagtttt tgtatagaca ttttatcacc atcctttaaa
      901 aatattaaat ttaagattta tttaagattt acttataaaa tacttaagtc ttatactcta
      961 aaatcaacat tatagacaaa cataacaaaa aggagaatat gtaattatga aaaaatttaa
     1021 attaatagga gccattattt tagtagtgtt tataagtgta tttatatcta aagagagatt
     1081 acttaagaaa gataaagatc caatagtagt tgctcaacat gattcagaac aagatgttga
     1141 ggcaaattct caaaataaaa atcactcaaa tagtatatca aatatattat tggtaaacaa
     1201 gacaaatgga attagcaaaa actatactcc tgaaaatatt actaaggtaa acataccttt
     1261 tgttgaagaa gcaacagagg aagaaaaaca aatggcagga gaacctgcaa aggcagttga
     1321 agatttagtt aagcaagcta attcagaagg aatacagttt ttagggtctt cagcatacag
     1381 atcttatgat actcaactgg atacttatac aagaagggta aaatctcagg ggcgagaaaa
     1441 ggcagatgca tatgtagcaa agcctggtta cagtgagcat caaactggac tttgtattga
     1501 tttaacaaat ccagaaagat ggtttgttgg aagtacaaaa gaagctaaat ggttggcaga
     1561 aaatgctcac aagtttggat ttataataag atatcctgaa ggtaaagaag atataacagg
     1621 tacagcatat gaaccatggc atattagata tgttggtaag gatgctgccg aggaaattta
     1681 ttcaaaagga ttaactttag aagaatattt acaaaataaa taatataaat gtgtaaataa
     1741 tttgtattat attggaaatt tagaaatgaa taaatttata gattacacta actaaaatat
     1801 agtataattt tatatgagta aagttatata caaagatgaa gttaggatgt gtaaaaatga
     1861 acaatacagt tcttgttgtg gatgatgaaa aggaaataag agaccttata gagatatatc
     1921 ttttaaatag tggatataat gtaattaaag ctgaagatgg aaaagaggct ctagatattt
     1981 taaaaaatga aaatattcat ttaatggtat tggatataat gatgcctaaa atggatggga
     2041 tagaagtttg tagaaaagtt agagaaagtt tgaatatacc aatacttatg ttaagtgcaa
     2101 aatcagaaga catggataaa atacaaggga taatgactgg tgcagatgat tatttgacaa
     2161 aaccatttaa cccacttgaa cttactgtga gagtgaaatc actgattaga agagcatatt
     2221 attttaatgg ttctaataat gaagataaag atgtcataga agttggagta gtaattatag
     2281 ataaggaaaa acatagtgta acagtagctg gaaatgaaat aattttgaca tcaagagaat
     2341 ttgatatatt atatttacta gcaaacaata aaggcagagt atatagtaca gaagaaatat
     2401 ttgaaaaggt atggaaagag aaatattacc aatctaataa tactgtaatg gttcatatga
     2461 gcagaataag ggataaaatt gaaaaatata cagatggaga aaaaataatt catactgtat
     2521 ggggagtagg atataaaatt gaaaaataaa agtttattaa atcatatgtt tcgacccata
     2581 ttagatctta taataagctg tggtttaact tcaatgctta ttttaacaag tatgtatata
     2641 ataaaattat tatatttcaa tagtgagata tctgtaatta atcaacttgt atatcagtta
     2701 agagatttca gaagtcttat aggagagagt ttattttatc catttttatt tacaatcaca
     2761 tcattagcaa tttattccat gatatcatac aagagaaata agcaaattgt agattttata
     2821 agaaagacta atagaaaaaa tcaaaaatta gaaatcatta aaagtgctgt aaatttaatg
     2881 gatgaaggta acttagaaaa aagtatagat attgagtatg acttagatat attaaaggat
     2941 aaagaaaagg atgatattga tgaactagca cataaaatca ataatatagt ccatcaactt
     3001 agaaatataa ctattgaaga gagacaagct cagcagacaa aaacagattt aataacaaat
     3061 gtgtcacatg acttaaggac acctttgaca tccataatgg gttatttagg agtgatagaa
     3121 gaaggtaagt ataaagatga agttcagatg atgtattatg ttgatattgc atatgaaaaa
     3181 tcgaaaaatt taaatgttct tataaatgat ttgtttgaat tgactaaaat gcaaaataat
     3241 acaataaaat taaacaaaat agaaataaat ttagtagagt tattaagtca agtagtatct
     3301 caatttgaaa tttattttaa acaagaattt atggttagta gaattaattt taatgaagaa
     3361 aaattaattg taaaagctga tccaaataaa ttggtaagag catttgaaaa tcttataact
     3421 aatgctgtga aatatggaaa agatggccat tatgtagata tagtcacaga gaaaaaggaa
     3481 gatatggcgg ttgtaaaagt gataaattat ggagaaccaa ttcctgtgtt agatttacca
     3541 aatatatttg atagatttta tagagttgaa aaatctagaa atagaaatgt tggaggttct
     3601 ggattgggtc ttgcgattac aaaaaatatt gtaaatttac ataatggaga aattactgtt
     3661 tcaagcgata aatatcaaac tgtatttgag gtacaactgc caataataca atcagcttag
     3721 ttataaaata aaagtacgat tatgtgatat aaatattata acaataaagt gtttacaaaa
     3781 ataaaaaagt aatatataat ttaagtcagt ttaataagat acttatcaag agttagggga
     3841 gagagttagc tcagtgatcc tataccaacc ttttatcagg tggtcctgct aacaacgatg
     3901 agatgacttg aaataatata ctcttcttat caataataga taggaagagt tttttttaga
     3961 attaatgtaa caaattttaa agtataatgg aggattaaat aatggaaaaa gtagaaagtt
     4021 ttaagctaga ccatacaaaa gtaaaagctc cttttgtaag aaagtgtagt gtacttgatg
     4081 gagtaaaggg agataaagta actaaatttg atttgagatt tttacagcca aatgtagaaa
     4141 gttttggaac tgctgctatg cacggtttag agcatctttt agctacttat ttaagagata
     4201 cattagatgg tgtaatagac ttatctccaa tgggatgtag aacaggattt tatttaatac
     4261 tatggggaga tgtagatgcc aaaacagtta agattggttt agaagaagca ttaaaaaaag
     4321 ttcttgaatc agataaaatg ccagcagcta cagctgtaga atgtggaaac tacagagact
     4381 tatctttatt tggggcaaag gaatatgcaa aagatgtttt agataaagga ttttctttaa
     4441 atatatatgg agaataattt aattggaaga gaagatataa gcataaatca aagtgaagtt
     4501 ttaagatatt tacaatacag aggtcaagag atagataaag accttaaata tactattgat
     4561 gaatgtgtag ctataactaa aaataaaata aatcctagat atttatctag gatttatcct
     4621 attagaataa ataaagataa tattgtagaa ttagagggaa caaacttaac cttagagagt
     4681 aaagatatat atgaactttt aaaagaatgt aatgagtgtg taattatggc agctactata
     4741 gggatagata tagaaaaaga aataagacga cactcttatt caaatttaac aaaaggacta
     4801 ataattgatt catgtggaac aacagcaata gaagaagtgt gtgacattgt tcaaaataat
     4861 ttagaaaagg tttttttaaa gcaagataaa tttataacta tgcgatatag cccaggatat
     4921 ggagatttac caatagaaaa aaatatagat atattaaacg ttttgaatgc tcaaaaacaa
     4981 ataggtctaa caattacttc taatggaatt atgataccga gaaagtcagt agtggctatt
     5041 ataggtattt ctaatacaag aataaataaa tctaagaaag aaaagtcgtg tgaaaattgt
     5101 aaaaactata gaaactgtac atatagaaaa ggagcgaact cttgtggaaa taagacaata
     5161 tctgaaaaat aatatattaa tatttgatgg tgcaatggga acaatgcttc aacaaaaagg
     5221 gttaaagtta ggtgaaaatc cagaagtatt tggtcttcaa aatccagata aacttataga
     5281 aatacataca gcatacttag aggcaggttc aaatgtaatt cttacaaata cttttggatg
     5341 taatgaacta aaattagata gtaagtatac tgtagaagaa gtgattgaca atgctgtttt
     5401 ggtggctaga aaagctatag aaaatgtaga taatactaaa ccaagatatg tagctttaga
     5461 tattggtcct ataggagaaa tgctagagcc tatgggtact ttaagttttg ataaggcata
     5521 tgaaatattt aaaagacaag tactacaagg cgttaagtct ggagttgatg tgatagtaat
     5581 agagactatg atggatttat atgaagctaa agtagctgtg ttagcagcta aagaaaattc
     5641 tgatttacct attttctgta ctatgacatt tgatgaaggt ggaagaagtt ttactggatg
     5701 tatgccagaa tgtatggtgg ctactataga aggtctaggt gtagatgcaa taggagttaa
     5761 ttgttcatta ggacccaaac aacttttacc aatagttgaa aaaatagcat caagagctac
     5821 agtccctgtt atggttcaag caaatgcagg tcttccaaat atagttgatg gagaggctat
     5881 atatgatgtt gatgcaaaag aattttttga aggggttaaa aagttcgttg aggttggtgc
     5941 tactataata ggtggatgtt gtggtacgaa tccaagtttt ataaaagaaa tatctgagaa
     6001 tataaattct gttacaaagg gatgtatcga gaaaattgat gaatgtgtag tctgttcacc
     6061 atcaaaattt gtggaagtac aatcgcctac agttgttgga gaaagattga atcccacagg
     6121 tagaaaatct ctacaagaag cattaaaaaa tgaaaatgtt gattatgcta taaatttagg
     6181 cttagaacaa gtaaatgctg gagcacaaat acttggagtg aatgttggtc ttccagagat
     6241 tgatgaaaaa aaactaatgc caaaattaat aagagagatt caagcagtag tagatacacc
     6301 tttacaagtt gattcttcca atgtagaggc cttagagcaa ggacttagat attataatgg
     6361 tagaacaata gtaaactctg taaatggcaa agaggagtcc ttagaaagca tattaccaat
     6421 agttaaaaag tatggttcat gtgtggttgg acttacactt gatgaaaagg ggatacctaa
     6481 aaaggctgaa gaaagatttg aaatagcaaa aaggatagtt aatagagcag tttcttatgg
     6541 gataaaacca aaagacatat ttatagattg tttatcatta actgtttcag cacaacaaga
     6601 agaagcaata gaaactataa aagctataac aatggtaaag acactaggag taaaaactat
     6661 attaggtgtt tcaaatattt catttggtct tccaaatagg aaagcactta atgcttcttt
     6721 tctaacatta gctttagggg cagggcttga tttagcaatt ataaatccaa atgaatatag
     6781 tatgatggaa gctatcaatt cttttaaaat attaaataac acagataaag gatgtataaa
     6841 ctatataaat caatatagta atataaataa ttcaaaaaaa tcggattcat ctacaaagat
     6901 tgacaaagat ttgcccttgg atattttagt tgaaagggga ttaaaagatg aagcaaaaaa
     6961 tataacactg aatcttttaa aagagaagga cgaaaattat atattagatg agattttaat
     7021 tcctgctctt gataaagtag gaaaaagata tgatagtgga gatatatttt taccacaatt
     7081 gattcaatca gcagaaacgg taaaagtttc attaaataca ataaaggaaa ctttattaag
     7141 taaaagtagc aataatgtat ctaaaggaaa aataatagtt gctacagtaa aaggtgatat
     7201 ccatgatatt gggaaaaata tagtaaaaat aatgctagaa aattatggat atgaggttat
     7261 agatttaggt aaagatgtcc ctattgaaga agtagtagaa gttgctaaaa agagaaatat
     7321 aaaattagta ggattaagtg cacttatgac tacgactgtt caaagtatga aagatacaat
     7381 tcaggcactg agagataatg agataaatgc aaaggtattt gtaggaggtg cagttttgac
     7441 agaagaatac gctgaagaaa tgggtgctga ttattattca aaggatgcaa agtctgctgt
     7501 tgaaatagca aagttaaatt tttagcatgt aaattaaaat aaaggtttta ctaaatagga
     7561 tattttatat ataatataaa tataagtttt agatacttat gtttaaaaca ataggaatta
     7621 atctataata atgagattaa ttaatataaa aaatatttat catcaacatt aaaaagggct
     7681 gggaagtctg gtataacttc catcagccct ttttagttaa aattatatgt taaaaagccc
     7741 aattaccatt tttaaaaatc tgtatttgct ttccatcata agtctcacca ataatatcca
     7801 tatcagcaga accaatcata aaatctacat gtgttaaact gtcatttaca ccatgttcat
     7861 ctaattcttc atcttttaaa ttgtttccac cttccagaca agtcttataa gcagagccaa
     7921 ttgcaaagtg gcaagaagca ttttcatcat atagagtatt ataaaaaatt atatctgtat
     7981 tggatattgg tgagttgtat ggaacaagag cgacttctcc taaataatga gagccttcat
     8041 ccgtttcaat aagtttttct agtgtatcta gtcccttttc agcagagaaa tctattattt
     8101 taccatcttt aaatgttaag aagaaattgt caattatatt tccaccataa acaagaggtt
     8161 ttgtactata aacagttcca tttactttaa acttatgtgg cattccaaaa atctcttcag
     8221 ttggaatatt cggattaaaa cttattccat tagggtcttt ggatgctcca cttagccaaa
     8281 catgaccctt tggcaagtct agagtcaaat cagtttttga ggatttatat tttaaagttt
     8341 taaagttatt tttatttaga aagtccattt tagatttaag attttcatta tgttcttccc
     8401 atgcttttat agggtcttga ccatctactc ttgaacattt aaatatagca tcccacagtt
     8461 tttctattgc tttatcttct gatagatttg gaaacacctt ttttgcccaa tcttttgttg
     8521 gcacagagac tatactccat ttacatttat cagttagagt ataagaacgc cattctttta
     8581 aagcttttcc ggatgccttc tgaaaatttg caactctttc agggtcaaca tcttttaata
     8641 aatctggatt ttgggcatag actgataaaa atgctccacc ttcttttgct atatctacat
     8701 attgttctgc tgtccatttg gggaactcat gaaatgcttc atctggagca tataagtatt
     8761 taataagagt actttcttca tcagaccact ctatataaac atttttagca ccacatttat
     8821 atgcttcttt aagtgcattt cttgcaaaat cagcacattc tattggtgac tttataagta
     8881 gaatttgacc tggctgtatg ttaactccaa cttttattgc taattcagcg tatttttcta
     8941 aatttctatt taaattcata tttatcctcc tatatttttg atattgaata acttataaag
     9001 tattataaca ttacttatgt ttttaatatt gaacaactta taaaatatta taactttact
     9061 tagattttgg gtcatattca aaatatttat actttataga ttgagaaata aaaaaaatat
     9121 tttaattaaa acagattatg aggataacaa acttatttaa gcagtatgtt ttttgctgat
     9181 aatagtatta aagtagaaat aaaaatactt ttttagtgaa aagctataca agaataccga
     9241 aaaaaactta taatataata atgagataga tatttataaa tgaaaaaaat ttttctggca
     9301 aagcttaatt aaaaagttaa tagctagtgt tgttatttaa attagcatag aaaatcaaat
     9361 aaaaaaatta tgctaatgta tagacattga taaggtagaa tttaagcctt ttgaatgcta
     9421 tattaaacta gagtagatat tcttatgaat ttcaaaatag ggcaggagga aaaaggaaat
     9481 aaaactataa aaaatataaa gtatttatat aaatttatag agtttatacg tcagaagatt
     9541 tcagttagga gggcataaat ggagctttat gaagccattt tttatagaaa atctgtaaga
     9601 aatttttcca gtaagaagat aaaaaagcca ttgatggaag aaataaaaag aagttgtaaa
     9661 aatttagaat acttaaatga agatttaaat attaaagcac atgttgttga tagaggtcat
     9721 ataatacatt ttttgatggg taaggaatgt aaagtaaagg cacctcatta tatagtagtt
     9781 acctcagata aaggtgagga ctatttacag aatataggat ttgctacaga aggtgtagtt
     9841 ttgcgcctta caacattggg aatagctact tgctggttgg aaggaaactt aaagagagaa
     9901 gatatcttgg aatttgtaga tattgggaat aagaattttg aagaagatga ggaagatgat
     9961 ttagattatc tagctggaat aaaaacagaa gaagatgatg aaaacaaaat agaaaatcct
    10021 tatataataa tagcatttgg atatccagca aaaaatgaag acttgtttag gactagatat
    10081 gctagacctg atagaaagcc agtaaaagat atgataaata agataaatag gaatagaaag
    10141 tggaaagata tcttagaatt gacaagaaga gctccatctg taaaaaattc tcaaccgtgg
    10201 tatttccata aggatgaacg aggtcttcat ttatttgaaa agagacctaa gaaacactgt
    10261 gaagatatga ataaggttag cttaggtgta gccttaagac attttgatat agcttgtata
    10321 aaaaataaaa tagatgtttc atatgaaaaa cttcctataa ggaataaaat aggaaaatca
    10381 tattttataa ctgtagtcga gcatgtgaaa ccagaagaag aaatccaaga agaaaatgta
    10441 atcctagaaa aagaggaatc tcaagatgaa taaaattttg gaaggggata ttggatatgt
    10501 ataagtgtgg aaaatgtaat cttagaggct gttctaatgg aaatatggat aaattaatga
    10561 agatatgtcc aactagagaa attgaactac aacaaaaagc aaagcaactt tatgaagaag
    10621 aagagaatag aaaaatagct tataatgctg gattagttga agcagagggg tattgtcaat
    10681 atactagact aaaagagatt atagagtttg ctaaaaagtg tggttataaa aagattggca
    10741 tagcattttg tattgggcta aaaagggaaa tgcaaacagt acaaaaaata tttgaacatc
    10801 atggatttga agtattttct gttatatgta aaaatggagc aatgcctaag agtacaattg
    10861 gactagatgc atctcaaaca atatcaaagt gttcagatga ggtaatgtgc aatccaatag
    10921 gacaagcttt atttttaaat gaagctaagg ttgaccttaa tgtaattctt ggattatgtg
    10981 ttggtcacga tactttagcc atgaaatata tggaagctcc tataacaact ttagttgtca
    11041 aagatagagt aactgggcat aatccagttg ctgtaatata taatgcagaa tcatattttg
    11101 agaaaaaatt gtttgataaa gaagaataat tcaaagactt attgacataa aaagcaatta
    11161 gtggtttaat tataaaaaag ataaaacttt aaattatcct agtgatacat aaagttatat
    11221 aaataaagaa aaatattaca gctaaattcc ttaaagtagt ccagagagac taaagggtta
    11281 tagatagtgt tagtatataa cctctagtaa atcttctaga ggtttttgat gagtataaaa
    11341 taaaaattta aatataataa agtcttttaa actagtccag agagactggg aaagatatct
    11401 catagctaat gaatagagtt atgtatagat aaaatccttg tgcctctttt ggtgtgagga
    11461 tttttttatg attatttagg ctttattaaa aaggagatgt aaaaatgata aatggtaaag
    11521 aaaagttgat agttgcaatt gatactgacg aatttgataa agctaaagaa ctaatagaca
    11581 gattagaaga tagtgtagac atatttaagg tggggctaga gcaatacgtt gccacaaaag
    11641 gtaaaacaat agattatctt aaagaaaaag gaaaaaaaat atttttagac cttaaattcc
    11701 atgatatacc aaatactatg aaaagtgcag tgagagctgc agtgagagat aatgtttggt
    11761 taatgacaat acatgtatca gatatggaag gaatgagaca atgtgcacta attgctaggg
    11821 aagaagcaga aagattaaat atagaaaagc cattagtagt tggagtaacg gttctaactt
    11881 cattaagcaa ccaagattta caggacatag gatgtaatat gacaacagaa gagttagcta
    11941 taaagagagc taaactggca aaagaatcag gaatagatgg ggttgtttgt tcagcacaag
    12001 aagtagataa aatagtagaa gcatgtggaa ctgattttat aacagtttgt ccaggaataa
    12061 gacctaaatc agcagaggtt ggagaccaaa aaagagttgt aactccatca gatgcaataa
    12121 aaaaaggtgc tcattattta gttgttggaa gacctataac aaaagcagaa aatccaagag
    12181 aatcagctat taatattgta agagaaatag aaaatgctta gataaaatat ttgggaggta
    12241 gcttacatga aggggaaatt aattttagaa gatggaacag tatttgaagg aaaagcattt
    12301 ggatatctaa aggaaactat tggagaagtt gtatttaata cttcaatggc tgggtatgga
    12361 gaactattga cagatgcaac ttattatgga caaatagtta ctatgactta tccacttata
    12421 ggaaactatg gaataaattt agaagatgtt gaatcaaata aggtgcaagt aaaaggactt
    12481 attgtaagag aaaaaagtga taacccaaat aattttagat gtgaaatgga tatagacaca
    12541 tacctaaagc aaaataaggt aataggactt gaaggaatag atacaagagc attaactaaa
    12601 attattagaa acaatggaac taagagagga attataactt tagaaaatgt atcattacaa
    12661 gatgttcagt ctaaattaga agagcattgt aacaaagatt cagtaaaaaa tgttacaaga
    12721 aaagaaattg aaaatataaa aggtactggt gctaaggttg tagttataga ctttggtgtt
    12781 aaaaatagtg tactagagtc tcttaaagct tgtaactgtg atataactat agtaccagca
    12841 atgactacag tagaagaaat tttaaatata aatcctgact taatcttatt atctaatgga
    12901 ccaggaaatc cagaggatat aaatgatata gttgaaaata taaaaaaatt aataggcaaa
    12961 aagccattga caggaatagg tttaggacat caaattttag cattggcatt aggaggaaag
    13021 acttcaaagc ttgtatttgg acatagagga ggcaatcacc cagttaaatg tttagaaact
    13081 ggaaaggtat ttataacttc tcaaaatcat ggatataatg tttcagaaat gccagaaaat
    13141 acagagataa ctcacataaa tttaaatgac aatactatag aaggaatgag acataaggaa
    13201 ttaccaatat atagtgttca atacattcca gaatacacaa caaaagaaga tttagattgt
    13261 atatttaata aattcttagc attaatatag atttagtatt atttacagat agtaaaaacc
    13321 atttagataa ggagaaaaat ttatgcctaa attagatagt ataaagaaaa ctttagtatt
//
LOCUS       DAEPXF010000427        11234 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain HB27
            SAMN13376389-rid12479423.guided.130, whole genome shotgun sequence.
ACCESSION   DAEPXF010000427 DAEPXF010000000
VERSION     DAEPXF010000427.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13376389
            Sequence Read Archive: SRR10518432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 11234)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11234)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001069624.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001069624.1
            Genome Coverage       :: 315x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/15/2021 01:54:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,165
            CDSs (total)                      :: 4,076
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 89
            rRNAs                             :: 10, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 10 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 19 of 69
            Pseudo Genes (incomplete)         :: 44 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 10 of 69
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11234
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13376389-rid12479423.guided.130"
                     /strain="HB27"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="China: Hebei"
                     /collection_date="2017"
     gene            48..746
                     /gene="vanR"
                     /locus_tag="KRL67_004149"
     CDS             48..746
                     /gene="vanR"
                     /locus_tag="KRL67_004149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002594737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanR-ABDEGLN family response regulator
                     transcription factor"
                     /protein_id="HBH1524914.1"
                     /translation="MNGKILVVDDERDIADLIEVYLTNENYIVYKFYSGLDALNCINL
                     NEFDLAILDVMLPGVNGFSLCQKIREKYTYPIIMLTAKDEETDKITGLTLGADDYVTK
                     PFRPLELVARVKAQLRRYKKYSGSLQSGEELSHIFSFLGLELNTQTYECHLNGKALSL
                     TPTEFSILHVLLKNKGSVVNSENLFHAIWKDEYYNKSNNTITVHIRHLREKLGDTVDE
                     AKYIKTIWGVGYKI"
     gene            782..1897
                     /locus_tag="KRL67_004150"
     CDS             782..1897
                     /locus_tag="KRL67_004150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBH1524915.1"
                     /translation="MKKINNFTYRKLRDKYEFIKREKNTEAYLKYKLYIMLAIYTGIV
                     LVLMFMADYTFSNFSNPLVKWLKLRRDVLFFLVFIIGYITIFNFYWKKPWNYLREIIN
                     ATHTIYQQNDELVELSKPLKEIEQQMNQIKMSVLLSQQAVKEAENKKNNLVMYLAHDI
                     RTPLTSVIGYLSLLKEIPDMDNSQKSKYTEVALDKAQYLEKLINELFEITRYNSNQIK
                     INFEKVDLYYMLLQLKDEFYPLLSEKGNRAILKANESLTIKADSEKLARVFNNILKNA
                     IAYSYPNTEIIISAVHKDSKVDIAFRNYGQTIPEEQLSAIFEKFNRLDEARKSDMGGA
                     GLGLSIAKEIINLHGGDITVQSKDEVTTFTISLPTSI"
     gene            2042..2929
                     /locus_tag="KRL67_004151"
     CDS             2042..2929
                     /locus_tag="KRL67_004151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBH1524916.1"
                     /translation="MELKIEHVTKKFKDKIAVEDVCLTLTPGVWGFLGANGAGKTTLM
                     RIITDIMKPTNGKILYNGKNIYTMGAEYRNLLGFLPQEFGFPQDFSVKDYLEYIAALK
                     DVPPREVKGKINNLLEILTLSDVKNKKIIKLSGGMKRRVGIAQAMLNDPKILIMDEPT
                     AGLDPGERVRFRNFISEFSHNRVVLISTHIVSDIEYISTQNAIMKSGKILDVGTTDKL
                     VKKVEGKVWECLISNRDISKYEMNLRIINQRSEKNNQVSIRYLAEKSQIDNSIAVEPR
                     LEDLYLWLFPEAVYSKEGK"
     gene            2930..4165
                     /locus_tag="KRL67_004152"
     CDS             2930..4165
                     /locus_tag="KRL67_004152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBH1524917.1"
                     /translation="MRIFYLEIKRVLHSRRTLILLLLALVLSALMAFLPITYEGINYL
                     DDNGKVVELSGLDAIKYKKTIYANNSEISSGVVTPKKLKHALNIWKKTEEKYGKVGSE
                     DFPLDVYSKSILPIRPLLNRLPEAFANSETGFATELSEINTNDLDSFYEQCKTHLNDI
                     MKIERQTSSAQMKAQELYRKVDTPFEIYPGYSKDAFDYIIFYIFILLIICVAIAAPTF
                     SGEYQTQSDSILRCTKHGHIRLAFTKILALFSIFIIMFVICISIHLAISDLAFGTECL
                     KTSFQMLFSVISLVNINLLQAQILIAIGGLISILAMVSLTLFLSAKCKDSLTSMLIAF
                     SICLIPMGIGVSWIGYLLPPGGIGFKISLLYQMTSFNFLHLGGMSFWTPHIILFFSVI
                     EIPIFIFLAIRTYCKHQVS"
     gene            4547..5464
                     /gene="pstC"
                     /locus_tag="KRL67_004153"
     CDS             4547..5464
                     /gene="pstC"
                     /locus_tag="KRL67_004153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HBH1524918.1"
                     /translation="MIVKSRLVGLEKNDNNGNKTKYVIEKVAKNIFLLSALVAVASLL
                     LIIGFVFYKGLRPFIFEGYSFIDFLTGADWIPSANKFGISSMIVASIFATLGALVIGV
                     PVGILTSVFIAEVAPKRLAKIMSPAVELLAGIPSVLYGVFGLAVIVPTIQSIFNLPKG
                     QSLLAIVIVLAVMMLPTIISVSETAIRAVPKAYKEGSLALGASKIETIFKVVLPAAKS
                     GVLAAVVLGVGRALGETMAVILVAGNSPVMPTVITDSVRPLTTNIALEMGYAFGTHQE
                     MLFATGVVLFTFILILNLVLNKLSNKAVN"
     gene            5466..6290
                     /gene="pstA"
                     /locus_tag="KRL67_004154"
     CDS             5466..6290
                     /gene="pstA"
                     /locus_tag="KRL67_004154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003460575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HBH1524919.1"
                     /translation="MRKFKENLLKSLVYLSALFTIVSLVVIVGFIFIKGIGNMNFNFL
                     FSNYSASGDGGILPMIITTLYTVVVSIVVATPIGILAAIYLQEYAKKGKAVTAIRFAT
                     ESLSGIPSIVYGLFGGIFFVVTLKMGYSIVAGSLTVAIIILPVIIRTTEEALKTVPQA
                     YRESSLALGATKFQTLYKVVLPSAIPGILSGVILSVGRIIGESAAILLTAGTVAKMPG
                     GIFDSARTLTVHSYLLTKESGDIATAASIGIVLIVIVLALNMLARFVAKKLNKANY"
     gene            6309..7073
                     /gene="pstB"
                     /locus_tag="KRL67_004155"
     CDS             6309..7073
                     /gene="pstB"
                     /locus_tag="KRL67_004155"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HBH1524920.1"
                     /translation="MELIDKIKMSVKDLDLFYGDKQALKKINMDIKENKVTALIGPSG
                     CGKSTFIRTLNRMNDLIEDVTIKGNISVDGEDIYTSDDVINLRTKVGMVFQKPNPFPM
                     SIYDNVAYGPRTHGLRDKKQLDKIVEESLKGAAIWDEVKDRLKSSALGLSGGQQQRIC
                     IARAIAMRPEVILMDEPTSALDPISTLKVEELIEDLKKDYTIVIVTHNMQQAARISDE
                     TAFFLNGEVIEFSDTKTMFTTPVDKRTEDYITGRFG"
     gene            7101..7754
                     /gene="phoU"
                     /locus_tag="KRL67_004156"
     CDS             7101..7754
                     /gene="phoU"
                     /locus_tag="KRL67_004156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HBH1524921.1"
                     /translation="MVNNSLDISIDTLKEQTVLMMEKCEEAIEKSVTCMIEKDLEGAR
                     KVVEDDEEINKLREEIRDKSIELIVLKQPMARDLRFIYSLGNISIELERIGDYAANIA
                     REMLKIGGEAYIKELIDIPKMAKECKSMMSQTREALILFDASIAYDVGTKDDIVDGLY
                     EQVQIDCLKIMHENSNTIDQGVKLLLIGRYLERIGDHITNVCEKIIFAIEGKMVEIG"
     gene            7913..8782
                     /locus_tag="KRL67_004157"
     CDS             7913..8782
                     /locus_tag="KRL67_004157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HBH1524922.1"
                     /translation="MKLQKMGMRTIKTGISVTLCMILGDYLVENMFYSLVACIISVQD
                     TVKGSLKTGINRVEGTILGGIIGFIFAIIKAGDPFLCGLGVMCSIYLCNLFKIKSVVV
                     ACVTFLAIHLGVGNSDPVYYSLHRVLDTSVGVLFGVLVNYYVARPNYLNSVIDEFKKI
                     EELSMLLIEDKILRKENLNVEKFEEEVEKLEEIYSKLIDELDYSLSETNIEKIENALR
                     ICREIHFHMQSIELLENKLYLKEHTYKYLKKTYNVDKLDWNIDEEKSPVFNYHLMKIM
                     IEIELLRKLNKSY"
     gene            8869..10305
                     /locus_tag="KRL67_004158"
     CDS             8869..10305
                     /locus_tag="KRL67_004158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron hydrogenase"
                     /protein_id="HBH1524923.1"
                     /translation="MNTRNDNSKILEIYKMKLFGELVKAVWNDDIEDIENLPNKILNE
                     DLKDNLNNYFSGKFDKDTMVNLIRVTMGLDVTEDTDKNLKESLHEAVNLDEVKRPIIS
                     VISDACKYCDENNKEDESCKIRSKHINCNEEDVCSACGDCVSRCKLGAISDKIEFIPM
                     IKLLKDIKSPVYAIVAPAFVGQFGKNVSPGKLRTALKKIGFEDMIEVALAADMLTAKE
                     AYDYYEHMKKDEEGCFITSCCCPIWVSLIQNSFPQILENVSPSVSPMIACGRAVKILN
                     PDAKVVFIGPCMAKKKEAMLEDIKDAVDFVLTFKELQEIFDALELNPAIMDESDRVEA
                     SLSGRVYARTGGVSKAVKLSVRRIDKNIKFEEKAFEGTKECMDGLKKVLNKEIEATFI
                     EGMGCVGGCVGGPKRILSVEEGTKNVDEYSESTNMETPFDNLNVLQFLTSLGIKRVES
                     LGEKEEEKVKKIFSRDIKSNKKENKYKK"
     gene            10353..11180
                     /locus_tag="KRL67_004159"
     CDS             10353..11180
                     /locus_tag="KRL67_004159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HBH1524924.1"
                     /translation="MKIKDNKAVFHVFIRIFLIAVLILTGTLLEEKINNVPVENISSK
                     GENTLELLSQSNTLGEIIYKASQEETKGPGATSEKVAYITIDDGPSKYTNALLDILKE
                     NDVKATFFMLNQNMKNHKEEVRRVLEEENSIGFHSVSHDIHKLYKSPEVTVGEFNTCN
                     NTLQEITGQSSKLIRLPYGSKPYMPEGSYNKLMANDYLIWDWNLDTQDWKSSTSQIVS
                     NILYYGRKRDELVVLIHEKEQSLNALNNIIRILKERGYTILPITENITPKNFWQENL"
BASE COUNT         4321 a         1253 c         2052 g         3608 t
ORIGIN      
        1 tttagtataa tatatataaa tatatataga aaggatgttt aaagtctatg aatggaaaaa
       61 ttttagttgt agatgacgaa cgtgatatag ctgatttaat agaagtctac cttacaaatg
      121 aaaactatat tgtttataaa ttttattctg gtcttgatgc actaaactgc attaatttaa
      181 atgaatttga ccttgcaatt ttagatgtta tgttgccagg agtaaatgga ttttctttat
      241 gccaaaaaat ccgagaaaaa tatacttatc caatcattat gttaacagct aaggatgaag
      301 aaaccgacaa gattacaggc ttaactttag gtgcagatga ttatgttact aaacctttcc
      361 gacctttgga acttgttgct agagttaagg cacagcttag aagatataag aagtacagtg
      421 gaagtttaca atctggtgaa gaactttcac atattttttc ttttttaggt ctggaattga
      481 atacccaaac atatgaatgt catttaaatg gtaaagctct ttcacttact ccaacagagt
      541 tttctattct acatgtatta ttaaaaaata aaggctctgt ggtaaattca gaaaatcttt
      601 ttcatgctat ctggaaagat gaatattaca ataaaagtaa caatactata acagttcata
      661 ttcgccatct tcgtgaaaag ttaggtgata ctgtagatga agcaaaatat ataaaaacaa
      721 tttggggtgt tggttacaag atttgacacc tatttttctt gtcaagaaag gatgtagtat
      781 tatgaaaaag attaataatt ttacttatag gaaactaaga gataaatatg aatttataaa
      841 aagagaaaaa aatacagagg cgtatttaaa gtataaatta tatataatgt tagctatcta
      901 tacaggtata gtattggttt taatgtttat ggctgattat acgttttcaa atttttcaaa
      961 tcctcttgtt aaatggctta agttgagaag agatgtatta ttttttttgg tttttataat
     1021 tggatatatc actattttca atttttattg gaaaaaacca tggaattatt taagagaaat
     1081 catcaatgca acacatacta tttatcaaca aaatgatgaa ttggttgaac tatctaagcc
     1141 attaaaggaa atagagcaac aaatgaacca aattaagatg tctgttttgt taagtcaaca
     1201 agcagtgaaa gaggctgaaa ataaaaagaa taaccttgtg atgtatctag cacatgatat
     1261 aaggacacca cttacttcag ttattgggta tttgagctta ttaaaagaaa tacctgatat
     1321 ggataattct caaaaatcta agtatactga agttgcactt gacaaagcac aatatttaga
     1381 aaaacttatc aatgaacttt ttgaaataac acgatataat tctaatcaaa tcaaaattaa
     1441 tttcgaaaaa gtagacctat attatatgtt acttcagtta aaggatgaat tttacccatt
     1501 gctatctgaa aaaggaaata gagccattct aaaagcaaat gaaagtttaa caataaaagc
     1561 agattctgaa aaacttgcaa gagtatttaa taatatcttg aaaaatgcta ttgcttacag
     1621 ctatcctaat acagaaatta ttatctcagc ggttcataaa gacagcaagg tagatattgc
     1681 ttttagaaat tatggacaga caattccgga agaacaactt tctgctatat ttgaaaagtt
     1741 caatcgttta gatgaagcta gaaaatctga tatgggtggt gcaggtttag ggctttctat
     1801 agctaaggaa attataaatt tgcatggtgg tgatattact gttcaaagta aagatgaagt
     1861 cacaacattt actatatcgc ttcctacatc tatttagtaa aatataaatc aaatattagg
     1921 attttcttaa gaaattatct atttaatatt aaaactatga gtgtctttgt ttagtaaaat
     1981 aaatactgag caaggcactt ttgtttttgc aggtaagtat ataaagagag gagtttcaaa
     2041 tatggaatta aaaattgaac atgtgacgaa aaaatttaag gataaaattg ctgtagaaga
     2101 tgtttgcctt actttaacac caggtgtgtg gggatttcta ggagcgaacg gagcaggtaa
     2161 aactaccctt atgagaatta ttacagacat tatgaaacct accaatggca aaattctgta
     2221 taatggaaaa aatatttata caatgggtgc tgagtacaga aatttactgg gatttttacc
     2281 acaagaattt ggtttcccac aggatttttc tgtaaaagat tatttagaat atatagcagc
     2341 attgaaagat gtaccaccaa gagaagtaaa aggaaaaatt aataatctat tggaaatcct
     2401 tacattatct gatgtaaaaa ataaaaagat tattaaatta tctggtggaa tgaaacgacg
     2461 agtgggaatt gcacaagcca tgttaaatga ccctaaaatc cttataatgg atgaaccaac
     2521 agcagggctt gaccctggtg agcgagtgag atttcgtaat tttatttcag aattttctca
     2581 taatcgtgtt gtcctgatat ctactcacat tgtatctgat attgagtaca tatcaactca
     2641 aaatgctatt atgaaaagtg gaaaaattct tgatgtggga acaaccgata aactggttaa
     2701 gaaagtagag ggaaaagtat gggaatgtct tatatcaaat agagatattt caaaatatga
     2761 aatgaattta cgtataatca atcagcgtag tgaaaaaaac aatcaagtat ctattcgtta
     2821 cttggcagaa aagtcacaaa ttgataactc tattgctgtt gaaccacgat tagaagattt
     2881 atatttatgg ttatttccag aagcagttta tagtaaggag gggaaataaa tgcgtatttt
     2941 ttatttggaa attaaacgag tgttgcactc acgtcgtaca ttaattttat tattgttggc
     3001 gttggtttta tctgcattga tggcttttct tccaattact tatgagggta tcaattattt
     3061 agatgataat ggcaaggtag ttgaattgag tggtttagat gctattaaat ataaaaaaac
     3121 aatttatgct aataatagtg aaattagtag tggagttgta acaccaaaaa aattaaagca
     3181 tgctttaaat atatggaaaa aaacagaaga aaagtatggt aaagtaggta gtgaagattt
     3241 tccactagat gtttattcta aaagcatttt accaattcgc ccattgttga atagacttcc
     3301 tgaagcattt gctaattcag aaacaggatt tgcaacagaa ttatcagaaa taaatacaaa
     3361 tgatttagat tctttttatg aacaatgcaa aactcattta aatgatatta tgaagattga
     3421 acgacaaact tccagtgcac aaatgaaagc tcaagaactt tatagaaaag tagatactcc
     3481 atttgagata tacccaggtt attctaaaga tgctttcgat tatataatat tttatatttt
     3541 tatattatta attatatgtg ttgcaattgc tgcacctact ttttctggtg agtatcagac
     3601 acaatcagat agcatccttc gttgtacgaa acatggacat attcgtctag cgtttactaa
     3661 aatattagct ctattttcta tatttataat aatgtttgtt atatgtattt ctatccattt
     3721 ggcaatttct gatttagctt ttggtactga atgtttgaaa acctcatttc aaatgttgtt
     3781 ttctgtcatt agtctagtaa atataaattt actacaagca cagattctta tagctattgg
     3841 tggtctaatt tcaatacttg caatggttag tttaacatta tttttatctg caaaatgtaa
     3901 ggattctctt acttctatgc tgattgcttt ttctatttgc ttaattccta tgggaatagg
     3961 tgttagttgg attggatacc tattaccgcc agggggaatt ggttttaaga ttagtttatt
     4021 atatcaaatg acatcattta atttcttaca cttaggaggt atgagttttt ggacaccaca
     4081 cattatactg tttttctcgg tgatagaaat acctattttt atatttttgg ctattcgtac
     4141 ctattgcaaa catcaagtat cgtaacaatt tgatggaaat aatagtgata taaactagag
     4201 gatacaataa ttatatatgt agtataaaag ttaaatttag ttaacaaatc ttaatagcag
     4261 atataggtaa taaattatac ataaactgta ttattatcta tatctgctat tttaatatag
     4321 agaaacaaca gtatattgta aagtaaatgt caaatatagt tttaaaagta aaattaatgt
     4381 taaaaaagta aatataattt tacacttgct taacttaatc ctaatataga tttaacttaa
     4441 aaaaagtaga ataaaagtac aggaaatttg atgaagacga attataaaaa attaaatttt
     4501 gaatacagaa cgaaaatcga taagtatatt ttaatgggag ggtattatga tagtaaaatc
     4561 tagattagta ggtttagaaa aaaatgacaa taatggcaat aagacaaaat acgtaattga
     4621 aaaagtagca aaaaatattt ttctacttag tgctttagta gcagttgcaa gtttactttt
     4681 gataatagga ttcgtatttt ataaaggatt aagaccattt atatttgaag gatattcatt
     4741 tatagacttt ttaactggtg cagattggat tccttctgct aataaatttg gaatatcatc
     4801 tatgatagta gcatcaatat ttgcaacact tggagcactt gtgataggag tacctgttgg
     4861 tatattaacc tctgtattta tagcagaagt agcacctaaa agattagcaa aaataatgtc
     4921 accagcagtt gaacttttag caggtatacc ttcagtgtta tatggtgtat ttggtcttgc
     4981 agtgatagtt ccaactatac aaagtatatt taatcttcca aaaggtcaaa gcttgttagc
     5041 aatagtaata gtactagcag taatgatgtt accgacaata atatctgtat cagaaacagc
     5101 aataagagca gtaccaaaag catacaaaga aggttcatta gcacttggtg catcaaaaat
     5161 agaaacaata ttcaaagttg tattacctgc tgcgaaatct ggagtattag ccgcagtagt
     5221 gttaggagta ggaagagctt taggtgaaac aatggcagta atacttgttg caggcaactc
     5281 accagttatg ccaactgtaa taacagatag tgtaagacca cttacaacta atatagcact
     5341 tgaaatggga tacgcgtttg gtacacatca agaaatgcta tttgcaactg gagtagtatt
     5401 gtttacattt atattaattt taaacttagt tttaaataaa ctttcaaata aggcggtaaa
     5461 ttaagatgag aaagttcaaa gagaatctac tgaagtcatt agtatatcta tcagcactat
     5521 ttacaatagt atcattagta gttatagtgg gatttatatt tataaaggga attggaaata
     5581 tgaattttaa tttcttattt agcaattatt cagcatcagg tgatggtggt atacttccaa
     5641 tgataattac aacattatat acagtagtag tttctatagt tgtagcaact cctataggaa
     5701 tattggctgc tatatattta caagaatatg ctaaaaaagg aaaagcagtt acagctataa
     5761 gatttgcaac tgaaagttta tcagggatac catctatagt atatggatta tttggaggaa
     5821 ttttctttgt agttacatta aaaatgggat attccatagt tgcaggttca cttacagttg
     5881 cgataataat attaccagta ataattcgta caacagaaga agctttaaaa acagttcctc
     5941 aagcttatag agaatcatca ttggcattag gagctacaaa atttcaaact ttatataagg
     6001 tagtacttcc aagtgcaata ccaggaattt tatctggagt aatcttatca gttggtcgta
     6061 taattggaga atcagctgca atattactta cagcaggtac tgtagccaag atgccaggtg
     6121 gaatatttga tagtgcaaga acattaactg tacactcata cttactgact aaagagagtg
     6181 gagatatagc aacagcagcc agtataggaa ttgttttaat agtaattgta ttagcactaa
     6241 acatgttagc aagatttgtt gctaagaaat taaataaagc taattattaa atttaagagg
     6301 tgtgaataat ggaattaatc gataagataa aaatgagtgt aaaagatttg gatttatttt
     6361 atggtgataa gcaagcatta aagaaaataa atatggatat aaaagagaat aaggttactg
     6421 ctttaatagg tccatctgga tgcggaaaat caacttttat aagaacgtta aatagaatga
     6481 atgacctaat cgaagatgta acaattaaag gaaacatttc tgttgatgga gaggacatat
     6541 atacctcaga tgatgttata aatcttcgca ctaaagtagg aatggtattt caaaagccaa
     6601 atccatttcc tatgagtata tatgataatg tggcatatgg tcctcgtaca catggactaa
     6661 gagataagaa gcaattagat aaaattgtag aagaaagctt aaaaggtgca gctatttggg
     6721 atgaggttaa agacaggctt aaatcatctg cattaggact ttctggtggg caacaacagc
     6781 gtatatgtat agcaagagca attgctatga gaccagaagt tattcttatg gatgaaccta
     6841 catctgcttt agacccaatt tcaacattaa aagtagagga acttatagag gatttaaaaa
     6901 aagactacac aatagtcata gttacacata atatgcaaca agctgctcgt atatcagatg
     6961 aaacagcttt tttcttaaat ggagaggtta tagaatttag cgatacaaaa actatgttta
     7021 ccactcctgt agataaaaga acagaagact atataacagg tagatttggt taaaatatat
     7081 aactataaag gagttagaca atggtcaata atagtttaga tataagtatt gatactttaa
     7141 aagaacaaac agtacttatg atggaaaaat gtgaagaagc aatagaaaag tctgtgactt
     7201 gtatgataga gaaggattta gagggtgcaa gaaaagtagt agaagatgat gaggaaataa
     7261 ataaattaag agaagaaata agagataaga gtatagaact aatagtatta aagcaaccaa
     7321 tggcaagaga cttaagattt atttattcat taggtaatat atctatagag ttggaaagaa
     7381 taggagatta tgcagcaaat atagctagag aaatgttgaa gataggtgga gaggcttata
     7441 taaaagagct gatagatatt cctaagatgg caaaagaatg taagtctatg atgtctcaga
     7501 ctagagaagc tttaatctta tttgatgcaa gtatagctta tgatgttgga actaaagatg
     7561 atattgtaga tggattatat gaacaagtac aaattgattg tttaaaaata atgcatgaaa
     7621 actcaaatac tatagaccaa ggggtaaaac ttctacttat aggaagatat cttgaaagaa
     7681 ttggagacca tataacaaat gtatgtgaga agataatttt tgcaattgag ggtaaaatgg
     7741 ttgagatagg ataaaaaaac tgctttaaag cagttttttt attgtctaaa ttaagaaaga
     7801 attgataaaa aaatatgatt aatgggtaaa tttaaaaaat tgtgatatac taggaacata
     7861 ttaaatatga ggatgcgtat tttatataag gtgcttttta ggaggtggaa ctttgaaact
     7921 tcaaaagatg ggtatgagga ctataaaaac aggaattagt gtcactttat gtatgattct
     7981 tggtgactat ttagtagaaa atatgtttta ttctctagta gcatgtataa tttctgttca
     8041 agatacggta aaaggttctt taaagacagg aatcaataga gtggaaggta ctattttagg
     8101 tggtataata ggattcatat ttgccattat taaagctgga gacccttttt tatgtggtct
     8161 aggagtaatg tgctcaatat atttatgcaa tctgtttaaa ataaagtcag tagtagttgc
     8221 ctgtgtaact tttctggcta tacatctagg tgtaggaaat agtgaccctg tgtattattc
     8281 tttacatagg gtgttagata catctgtggg tgttttgttt ggagtacttg ttaattatta
     8341 tgttgctaga ccaaattatc taaatagtgt tatagatgaa tttaaaaaga tagaagaact
     8401 ttcaatgttg cttattgaag ataaaattct tagaaaagaa aatttaaatg tagagaaatt
     8461 tgaagaagaa gtagaaaaat tagaagaaat atattcaaaa cttattgatg agttggatta
     8521 tagtttgagt gaaactaata tagaaaaaat tgagaatgcc ttaaggatat gtagagaaat
     8581 acattttcat atgcagtcta tagagttact tgaaaataaa ctttatttga aagagcatac
     8641 atataaatac cttaagaaaa catataatgt ggataagcta gactggaata tagatgaaga
     8701 aaaaagccct gtgttcaact atcatctaat gaaaataatg attgaaatag agttgttgag
     8761 aaagctaaat aaatcatatt aataatagta gttaaataat aaataaaaaa tagtatcttt
     8821 gtaaatatta aacatatata agtttaattt atggaggtac tattttttat gaatactaga
     8881 aatgacaatt ctaaaatact agaaatatat aaaatgaagc tttttggcga acttgtaaaa
     8941 gcagtgtgga atgatgatat agaggatatt gaaaacttac ctaataaaat tttaaatgaa
     9001 gatttaaagg ataatttaaa taattatttt agcggaaaat ttgataaaga tactatggta
     9061 aatttaatta gggtaactat gggattggat gtgacagaag atacagataa aaatttaaaa
     9121 gagtcactac atgaagctgt aaatttagat gaagtcaaaa gaccaataat atctgtaata
     9181 tcagatgctt gcaaatattg tgatgaaaat aataaggaag atgagtcatg taagataaga
     9241 agtaagcata ttaattgtaa tgaggaagat gtatgtagtg cttgtggtga ttgtgtatca
     9301 agatgtaagt tgggtgcaat ttcagataaa atagaattta taccaatgat aaaattgtta
     9361 aaagatataa agagccctgt atatgcaata gttgcaccag cttttgtagg acagtttggg
     9421 aaaaatgtaa gccctggtaa attaagaact gcattaaaaa agataggatt tgaagatatg
     9481 atagaagttg ctttggcagc agatatgctt actgcaaaag aagcatatga ttattatgag
     9541 catatgaaaa aggacgaaga aggatgcttt ataactagtt gttgctgtcc tatttgggtt
     9601 agtttgatac aaaatagttt tcctcagata ttggagaatg tatcgccatc cgtttctcct
     9661 atgattgctt gtgggagagc tgtaaaaatt ttaaatccag atgctaaagt tgtattcata
     9721 ggtccttgta tggcaaaaaa gaaagaagcg atgttagaag atataaaaga tgcagttgat
     9781 tttgtgctta catttaaaga gttgcaagaa atatttgatg cattagagtt aaatccagca
     9841 ataatggatg agagtgatag agtagaggcg tccctttcag gtagagttta tgctagaaca
     9901 ggaggtgtaa gtaaagcagt taagttatca gttagaagaa ttgacaaaaa tataaaattt
     9961 gaagaaaagg catttgaagg aacaaaagag tgcatggacg gtctaaagaa agttctgaat
    10021 aaagaaatag aagctacatt tatagaagga atggggtgtg taggaggctg tgtaggaggt
    10081 cctaagcgta ttttaagtgt ggaagaaggt actaagaatg ttgatgaata tagtgaaagt
    10141 acgaatatgg aaactccatt tgataattta aatgtattac aatttttaac ttctttggga
    10201 atcaaaagag tagaatcttt aggagagaaa gaagaagaaa aggttaaaaa aatatttagt
    10261 agagatataa aaagtaataa gaaagagaat aaatataaaa aataactttt taagtcttaa
    10321 acttatgctt ttataaaagg gagatatggg ggatgaaaat taaagataat aaggctgttt
    10381 ttcatgtatt tataaggata tttcttatag ctgttttaat tttaactgga acattattgg
    10441 aggaaaaaat taataatgtt ccagttgaaa atataagttc aaaaggtgaa aatacactgg
    10501 aactgctttc tcaaagtaat actttaggag aaatcatata caaagcatct caagaagaaa
    10561 caaaaggtcc aggggctact tctgaaaaag tagcatacat aacaatagat gatggtccat
    10621 caaaatatac aaatgcacta cttgatattt taaaagaaaa tgatgtcaaa gcgacatttt
    10681 tcatgcttaa tcaaaatatg aaaaaccaca aggaagaagt aaggagagtc ttggaagagg
    10741 aaaactcaat aggttttcat agtgtaagtc atgatataca taagttatat aaaagcccag
    10801 aagtaacagt tggggaattt aatacatgca ataatactct acaagaaata actggtcaga
    10861 gttcaaaatt aataagatta ccatacggaa gtaaaccata tatgccagaa ggttcatata
    10921 ataaacttat ggcaaatgat tatttaattt gggactggaa tttggataca caggattgga
    10981 agtcttctac aagtcagata gtaagtaata tattatatta tggaagaaaa agagatgaat
    11041 tagtagtatt aattcatgag aaagagcaaa gtttaaatgc tttaaataat attataagaa
    11101 tcttaaaaga gagaggatat acaatattgc caattacaga aaatataaca cctaaaaatt
    11161 tctggcaaga aaacttataa taaatttaaa tatggtaaaa gataaagtaa attatttata
    11221 aaataaaaca tata
//
LOCUS       DAEPXF010000428         6318 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain HB27
            SAMN13376389-rid12479423.guided.209, whole genome shotgun sequence.
ACCESSION   DAEPXF010000428 DAEPXF010000000
VERSION     DAEPXF010000428.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13376389
            Sequence Read Archive: SRR10518432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6318)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6318)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001069624.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001069624.1
            Genome Coverage       :: 315x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/15/2021 01:54:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,165
            CDSs (total)                      :: 4,076
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 89
            rRNAs                             :: 10, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 10 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 19 of 69
            Pseudo Genes (incomplete)         :: 44 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 10 of 69
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6318
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13376389-rid12479423.guided.209"
                     /strain="HB27"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="China: Hebei"
                     /collection_date="2017"
     gene            147..1013
                     /locus_tag="KRL67_004160"
     CDS             147..1013
                     /locus_tag="KRL67_004160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001255868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside 6-adenylyltransferase AadE"
                     /protein_id="HBH1524925.1"
                     /translation="MRSEKEVYDIVLNFAKTDKRIRMVTLEGSRTNTNIPPDDFQDFD
                     ITFFVTDMDSFTSDDKWLDIFGERLILQKPEDMELFPAVEKGFSYLMLFTDDVKIDLT
                     LLPLELIDEYFTWDKLVKLLLDKDNRIVKPPIPTDIDYHLQKPTQRMFDDCCNEFWNT
                     TTYVVKGLCRKEILFAIDHMNDIVRKELLRMISWLIGIKQGFHFSLGKNYKFMKQYVP
                     EELWERLMSTYNMDSYPHMWESFEQCMALFREVSSEVACQLDYQYPLYDEKISNYVIR
                     QKKKYGIEDDNK"
     gene            complement(1607..1732)
                     /locus_tag="KRL67_004161"
                     /pseudo
     CDS             complement(1607..1732)
                     /locus_tag="KRL67_004161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112778.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
     gene            complement(1949..4078)
                     /locus_tag="KRL67_004162"
     CDS             complement(1949..4078)
                     /locus_tag="KRL67_004162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBH1524926.1"
                     /translation="MFFRKNKSRRVRNKRKNNYVSKVSSVSSESFKKLALNNVKKSLK
                     NYLIYFITLVFGVIILYTFNSLDNDIAILSNNALLSSSISFVRGIVSIFSILVCIIFA
                     FLIIYANNYLMKQRKKEFGIYSILGMDRRDINKLMFREMMIIGIFSLIIGIVFGVFIS
                     KGLSIFALKMLGISSSGFSFSILTTIKTIVFFGIVLLLVNKFNKKTIKKYKLIDLLNA
                     NKKNEVSVAKGKKSNLILFFLSIVSIVLAYIIPLKMKISTFVLCICLILILVGIYLFF
                     MSVSDFIMLKIKKHRFIYYKNLNPFTVSQISSQIKTMSMSITMICMLLFSSLIVIPFG
                     ISFGTYLIDDLQEATPYDVTLSKLFMNGDNIVEYSTKKSDINSKIPSLKDELIANDFP
                     LISFSKSTSDLRIYNLKNISQYDFITNDKDKTIKLKDNKLHIVSISDYNSTRKQSGLK
                     EINLKDNEFAINCASENYRQFYEKYIKSQPQNYITLNGVKLKLNQTSLYTNSYNTNTV
                     PLDLGTIIVPDAVIKDLNPIMITLNIDYKERSSNLENKFMDSYYKFEEKIDNSEDYSI
                     LYKSVIDGEKRALNTSFSFVAIYIGIILLISAGAILALQQLIESTVNKERFKLLSKLG
                     VNKKDMKKAIFIQISILFAVPLILALINALFISKVLFVIMPFIAETGVITNMLLTLGL
                     IIIVYGIYFIASLLESLNIVNNSKEII"
     gene            complement(4101..4835)
                     /locus_tag="KRL67_004163"
     CDS             complement(4101..4835)
                     /locus_tag="KRL67_004163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003722224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBH1524927.1"
                     /translation="MNKILRVKNIKKYYKSRGNVTKAIDNISFEVERGEYIGIMGASG
                     SGKTTLLNCISTIDKTTEGNIYIGDKDITTMDEDELSKFRREELGFIFQDFNLLDTLT
                     AHDNIALSLSINGVNSKEIDNKIKKIANKLGVHEILNKYPYEISGGQKQRIACARAII
                     TNPSLILADEPTGALDSKSAKLFLENLEVLNHELKATILMVTHDAFTASYCKRILFIK
                     DGKIFNELVRGDNTRQEFFDRIIKVI"
     gene            complement(5216..6154)
                     /locus_tag="KRL67_004164"
     CDS             complement(5216..6154)
                     /locus_tag="KRL67_004164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBH1524928.1"
                     /translation="MKCTNFTFKGEEGLDIYTYKWEDENIKKTKAVIQIAHGMAETAQ
                     RYETFAKVLTENGYIVYINDHRGHGKTAKIIENVGHLAEKEGFRCLVEDMYTLTNIIK
                     KENEDLPIYLFGHSMGSFASQRYIMDYGNNLSGLILCGSNGKQGIILNFAHMIINREI
                     KKYGRRSRSNKINDLIFGGEIIRRNEKTKFDWLSRDKEQVEKYINDPFCGVVCSCGFF
                     YDLVQGLKEIEDKENLKKVPLDIPIYIISGDKDPIGKNGKGVLRLRDRYIKLGVKDVT
                     CKLYKDGRHELLNEINKEEVFEDIICWLNNKIEILR"
BASE COUNT         2078 a         1065 c          810 g         2365 t
ORIGIN      
        1 aaagccagcc gagccagtca acggtcaaga tgaacggcgc atacgcgccg ccgttgacag
       61 ccccgcccgc ctttgctgat gagcaatcaa ggcgggaaag ccctaaaatg gcttcacacc
      121 tatttcaata attggaggag ataaaaatga gatcagaaaa ggaagtttat gatattgttt
      181 tgaattttgc aaaaacagac aaacgcattc gcatggttac tttggaagga tctagaacaa
      241 atacaaatat tccgcctgat gattttcagg attttgatat tacttttttt gttacggata
      301 tggacagctt cacaagtgat gataaatggc tagatatatt tggtgaaagg ttgattctgc
      361 aaaagccgga agatatggaa ttatttccag ctgtagaaaa gggattttca tatttaatgc
      421 tgtttactga tgatgttaag atagatttaa ctttgctgcc gctggaactg atagacgagt
      481 attttacatg ggataaactg gtaaagttac tgttggataa agacaaccgt atcgtaaagc
      541 cgccaatacc aacggatata gactaccact tgcagaagcc tactcaaaga atgtttgacg
      601 attgctgtaa tgaattttgg aatactacaa catatgtagt aaagggctta tgccgcaaag
      661 aaattctttt tgctattgac catatgaatg atatagtacg aaaagaattg cttcgcatga
      721 tttcctggct gattggtatc aaacagggat ttcatttcag tttgggaaaa aactataaat
      781 ttatgaagca atatgtccca gaggaattgt gggaacgact tatgtccact tataatatgg
      841 attcctatcc ccatatgtgg gaatcctttg aacaatgtat ggcattgttc cgggaggttt
      901 cgtcagaagt ggcatgccag ttggattacc agtatccact atatgatgaa aaaatcagta
      961 attatgtgat tcggcaaaag aaaaaatatg gcattgaaga tgataacaaa taaaattttt
     1021 tcaatcgaaa aaatttattt tgattattca attttgaaaa actgaatacc tcaaaatcag
     1081 aaagagcgcg gacaagcact ttgagggatt ggccgccttg tccacccatt cagtgggacg
     1141 gcgggtggcg gtcaaggccg ggtgtaaacc cgttcatttc agccttgacg gttacccgct
     1201 gtcctgttac ttttccggga gtgtagccac aacttcggga cactttgtcc acaagtcgga
     1261 gcgtaggacg agggacgggg gctttcatat acgccctgtc tgctgatgaa aacagacctg
     1321 cgcttgcggc tccacgccac acaagcacag tcctgttttc ctgccgcccc tccccggcgg
     1381 caacggcatt ttcacggcaa cgccgacaga gcgtataaca cactacactt tgcttcgcaa
     1441 agtcgtgtgc caaatggggg cgttgccccc tttggaaacc cccacaagaa aaggcggtac
     1501 gctacccctc tacggggcgc ataccgcctt ttcttgttat ccagccctcc ggctgtatcg
     1561 gttgagcaaa agtcagcgtg ggaatggtgt ggtatcttta tctaagtgaa tccccactct
     1621 cccacttaga tactgctggt gttgacacac ctataaactt tgctaattgt tcttgagtta
     1681 aatttcgttc ttttctaaga tgctgtatga ctttacctat tttaatttgc atatatacct
     1741 ccctagtaca tttggtgtaa tttaagtata taaaaaaaat atttactagt aaatcgaggg
     1801 aaagttaaca aaatgtctgt aatttaacca ttggttatat tttaatctaa aaccgtatct
     1861 cattggcttg aaatatagaa tctattaatt aagctaactt aagtgtagct taattaatta
     1921 gattaacact aaatagtctt ttaaaaactt aaattatctc tttactatta tttactatat
     1981 ttaagctttc aagtaaactt gcaataaaat aaattccata tacaatgata ataagaccta
     2041 acgttagtaa catatttgta ataacacctg tctctgcaat aaatggcatt ataacaaata
     2101 agacttttga tataaacaaa gcatttataa gagctaatat taaaggtact gcaaataata
     2161 ttgatatttg tataaaaata gcttttttca tatctttctt atttactcca agtttactta
     2221 ataatttaaa tctttcttta ttaacagttg attctataag ttgttgcaat gctagtattg
     2281 cacctgctga tattaataat ataataccta tatatattgc tacaaaagaa aaggaagtgt
     2341 tcaaagctct tttttctcca tctataactg atttatataa aatactataa tcctcagaat
     2401 tatcgatttt ctcttcaaat ttatagtatg aatccataaa tttattttca agattactac
     2461 ttctttcttt gtaatcaata tttaatgtaa tcataatagg atttaaatcc tttattacag
     2521 catctggaac aattatggtt cccaaatcta aaggaactgt attagtattg taagaattgg
     2581 tatataagct tgtctgattt aatttaagct ttactccatt taatgttata taattctgtg
     2641 gctgactctt aatgtacttt tcataaaatt gtctataatt ttctgaggca caatttattg
     2701 caaattcatt atcctttaga tttatttctt ttaaaccact ttgctttcta gtagaattat
     2761 aatcactaat tgagacaata tgaagtttgt tatctttcag tttaattgtc ttatccttat
     2821 catttgttat aaaatcgtac tgacttatgt tctttaaatt atatatcctt aaatcacttg
     2881 tacttttaga aaatgaaatt aggggaaaat catttgctat tagttcatct ttaagtgaag
     2941 gaattttact atttatatca cttttctttg tactgtattc tacaatgtta tctccattca
     3001 taaataattt agataatgtt acatcataag gtgtcgcttc ttgtaaatca tctattaaat
     3061 atgttccaaa ggatatacca aaaggtatta ctatcaaaga agaaaataat agcatacaaa
     3121 tcatagtgat agacatactc attgttttga tttgactact tatttgactt actgtaaatg
     3181 gatttaaatt tttatagtat ataaatctat gcttctttat ttttaacatt ataaaatcag
     3241 atactgacat aaaaaacaaa taaattccaa ctaatattaa aattaaacat atgcatagta
     3301 caaacgtaga tattttcatt ttcaatggta ttatatatgc taaaacaatt gacactatgg
     3361 ataaaaagaa taatatcaaa ttagattttt tcccttttgc aactgaaact tcattctttt
     3421 tatttgcatt taataagtct attaacttgt atttttttat tgttttttta ttaaatttat
     3481 tgactaataa caatacaatg ccaaaaaata ctattgtttt aatagttgta agtattgaga
     3541 aagaaaatcc tgatgatgat atacctaaca tctttaaggc aaatatacta agtcccttag
     3601 agataaatac tccaaataca atacctatta ttaaagaaaa tatacctatt atcatcattt
     3661 ctctaaacat taatttattt atgtctcttc tgtccattcc aagaatactg tatattccaa
     3721 actctttttt acgttgtttc attaaatagt tatttgcata aataattaaa aatgcaaata
     3781 taatacacac tagaattgaa aaaatactta caatacctct aacaaatgat atacttgaac
     3841 tcaataaagc attattacta agaattgcta tatcattgtc taaagaatta aaagtgtata
     3901 aaataataac tccaaataca agggttataa aataaatcaa ataatttttc agactttttt
     3961 tcacattatt taaggccaat tttttaaatg attcacttga cacacttgac acttttgaaa
     4021 cataattatt ttttctttta tttcttactc ttcttgattt attttttcta aaaaacatgt
     4081 ttatcatcac ccccaagagc ttatattact tttattattc tatcaaaaaa ttcttgacga
     4141 gtattatctc ctctaaccaa ctcattgaat attttaccat cttttataaa taatatcctt
     4201 ttgcaataac tagctgtaaa agcatcatgt gtaaccatta atattgtagc ttttaattca
     4261 tgatttaaaa cttcaaggtt ttccaaaaat aattttgctg acttagagtc cagagctcct
     4321 gtaggttcat ctgctaatat tagtgatgga tttgttatta tagctcttgc acatgctatt
     4381 ctttgctttt gaccaccaga tatttcatat ggatacttat taagtatttc atgaacacct
     4441 aatttatttg ctattttttt tattttatta tctatttctt ttgaattaac tccattaata
     4501 gataaagaca atgctatatt atcatgtgct gtaagtgtgt ctaataaatt aaaatcttga
     4561 aatataaaac ctagttcttc tctacgaaat ttagatagct catcttcatc catagttgtt
     4621 atgtctttat ctcctatgta tatattacct tctgtcgttt tatctatagt agaaatacaa
     4681 ttcaataaag tagttttacc acttcctgaa gctcccataa tacctatata ctctcccctt
     4741 tcaacttcaa aactaatatt atcaatagct tttgtaacgt tacctctact tttatagtat
     4801 ttttttatgt tttttactct cagtattttg ttcatatcca ttacctccat taagttttat
     4861 tttgaaatta aaaacaaata aatttatatt ttttacgtat ttttttctta tcaatttcat
     4921 aaataagctt cttatattta tcaaaatcaa tagcacctac ttatttctag ttccttttct
     4981 agaataattt aattttctta agaagatgtg tacttaaatc aaaagatttt cttaagattt
     5041 taatacattc ttctcttgaa agtagtaaat atacacatca aaaaataaaa aagtgtttca
     5101 ctgataataa ataatcattt tgaaacactt tcttttctct tttacatata ttaaatatac
     5161 tttttcaaac atttccatag aatcaaattt aatattaata tataattata ttcttttatc
     5221 tcaatatttc tattttatta tttagccaac aaattatgtc ctcaaaaact tcttccttat
     5281 ttatttcatt caacaattca tgccttccat ctttgtaaag tttgcaagtt acatctttta
     5341 ctcctaattt tatatatcta tcccttaatc ttaagactcc tttaccattt tttcctatgg
     5401 ggtctttatc tccagatatt atgtaaatag gtatatcaag tggcactttt tttaagtttt
     5461 ctttatcctc aatttctttt aacccttgaa ctaaatcata aaaaaagcca caactacaca
     5521 ctactccaca aaatgggtca tttatatatt tttcaacttg ttctttatct ctacttaacc
     5581 agtcaaactt agtcttttca tttcttctta ttatttcgcc accaaatatc aaatcattta
     5641 ttttattact tctagaacgt cttccatact ttttaatctc acgattgatt atcatatgag
     5701 caaaatttaa aatgattcct tgttttccat tcgaaccaca tagtataaga ccagataaat
     5761 tattaccgta atccattatg tatctttgac ttgcaaatga acccatgcta tgtccaaata
     5821 gataaattgg caaatcttca ttttctttct ttataatatt tgttaaagta tacatgtcct
     5881 ctacaagaca tctaaatcct tctttttcag ctaaatgtcc aacattttct attatctttg
     5941 cagtttttcc atgacctcta tgatcattga tatatactat ataaccattt tcagtaagta
     6001 ccttggcaaa agtctcatac ctttgtgcag tttcagccat tccatgtgca atttggataa
     6061 ctgcctttgt tttttttata ttttcatctt cccatttata tgtatatata tctaaacctt
     6121 cctctccttt gaaagtaaag tttgtacact tcataattat ccctccgatt gattttattc
     6181 agtatattaa tttatatatt atattacaag tattttcaag tcaaaatctt tctaaaaaaa
     6241 tttaagaatt attaattcct catagaagtt atcattgtac atagaaattt ttacataaaa
     6301 ttatacaata atttaatt
//
LOCUS       DAEPXF010000429          647 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain HB27
            SAMN13376389-rid12479423.guided.408, whole genome shotgun sequence.
ACCESSION   DAEPXF010000429 DAEPXF010000000
VERSION     DAEPXF010000429.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13376389
            Sequence Read Archive: SRR10518432
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 647)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 647)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001069624.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001069624.1
            Genome Coverage       :: 315x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/15/2021 01:54:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,165
            CDSs (total)                      :: 4,076
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 89
            rRNAs                             :: 10, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 10 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 19 of 69
            Pseudo Genes (incomplete)         :: 44 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 10 of 69
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..647
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13376389-rid12479423.guided.408"
                     /strain="HB27"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="China: Hebei"
                     /collection_date="2017"
     gene            44..>647
                     /locus_tag="KRL67_004165"
     CDS             44..>647
                     /locus_tag="KRL67_004165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CatB-related O-acetyltransferase"
                     /protein_id="HBH1524929.1"
                     /translation="MTIPNLNKVYPRSNDYQTVYLKNVITRENIEVGDYTIYNDFYND
                     PRDFEKNNVLYHYPINNDKLIIGKFCSIACNAKFLMTSGNHSMKSNSTYTFPIFYEEW
                     KLDINHITDAWDNKGNIIIGNDVWIGYDSIIMSGIHIGDGAIIGTRAVVTNDVPPYSI
                     VGGVPAKIIKKRFNNDTISKLLEIKWWDWPYKKIQSNIKHI"
BASE COUNT          268 a           81 c          102 g          196 t
ORIGIN      
        1 gaatagtcaa aaacataata tatatcaaag gagagattta aatatgacta ttccaaattt
       61 aaataaggta tatccaagaa gtaatgatta tcaaactgta tatcttaaaa atgtaataac
      121 aagagaaaat atagaagttg gagattacac aatatacaat gacttttata atgacccaag
      181 agattttgag aaaaacaatg tattatatca ttaccctata aacaatgata aattaataat
      241 tggtaagttt tgctcaatag catgtaatgc aaaatttcta atgactagtg gaaatcactc
      301 aatgaaatca aactccacat atacctttcc catcttttat gaagaatgga aactcgatat
      361 caaccatata acggacgctt gggacaataa aggtaacatt ataattggaa atgatgtgtg
      421 gataggttat gattctataa ttatgtcagg tatacatatt ggtgatggtg caattattgg
      481 aacaagagca gtagttacta atgacgtccc accgtattct attgttggag gagtacctgc
      541 aaagattata aaaaagagat ttaataatga tacaatttca aaattattag aaattaaatg
      601 gtgggattgg ccatataaaa aaattcaatc aaacattaag catattc
//
